LOCUS       DAZFVO020000001        81225 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFVO020000001 DAZFVO020000000
VERSION     DAZFVO020000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 81225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 81225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.001"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..124
                     /gene="yqcG"
                     /locus_tag="ACHKNB_RS00005"
                     /old_locus_tag="pgaptmp_000001"
     CDS             <1..124
                     /gene="yqcG"
                     /locus_tag="ACHKNB_RS00005"
                     /old_locus_tag="pgaptmp_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HGU4193592.1"
                     /translation="ENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFIQI"
     gene            138..1010
                     /gene="ygcG"
                     /locus_tag="ACHKNB_RS00010"
                     /old_locus_tag="pgaptmp_000002"
     CDS             138..1010
                     /gene="ygcG"
                     /locus_tag="ACHKNB_RS00010"
                     /old_locus_tag="pgaptmp_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4193593.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLDSIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1070..2368)
                     /gene="eno"
                     /locus_tag="ACHKNB_RS00015"
                     /old_locus_tag="pgaptmp_000003"
     CDS             complement(1070..2368)
                     /gene="eno"
                     /locus_tag="ACHKNB_RS00015"
                     /old_locus_tag="pgaptmp_000003"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HGU4193594.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2456..4093)
                     /gene="pyrG"
                     /locus_tag="ACHKNB_RS00020"
                     /old_locus_tag="pgaptmp_000004"
     CDS             complement(2456..4093)
                     /gene="pyrG"
                     /locus_tag="ACHKNB_RS00020"
                     /old_locus_tag="pgaptmp_000004"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HGU4193595.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4321..5112)
                     /gene="mazG"
                     /locus_tag="ACHKNB_RS00025"
                     /old_locus_tag="pgaptmp_000005"
     CDS             complement(4321..5112)
                     /gene="mazG"
                     /locus_tag="ACHKNB_RS00025"
                     /old_locus_tag="pgaptmp_000005"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HGU4193596.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5183..5518)
                     /gene="mazF"
                     /locus_tag="ACHKNB_RS00030"
                     /old_locus_tag="pgaptmp_000006"
     CDS             complement(5183..5518)
                     /gene="mazF"
                     /locus_tag="ACHKNB_RS00030"
                     /old_locus_tag="pgaptmp_000006"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HGU4193597.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5518..5766)
                     /gene="mazE"
                     /locus_tag="ACHKNB_RS00035"
                     /old_locus_tag="pgaptmp_000007"
     CDS             complement(5518..5766)
                     /gene="mazE"
                     /locus_tag="ACHKNB_RS00035"
                     /old_locus_tag="pgaptmp_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HGU4193598.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5844..8078)
                     /gene="relA"
                     /locus_tag="ACHKNB_RS00040"
                     /old_locus_tag="pgaptmp_000008"
     CDS             complement(5844..8078)
                     /gene="relA"
                     /locus_tag="ACHKNB_RS00040"
                     /old_locus_tag="pgaptmp_000008"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HGU4193599.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8126..9427)
                     /gene="rlmD"
                     /locus_tag="ACHKNB_RS00045"
                     /old_locus_tag="pgaptmp_000009"
     CDS             complement(8126..9427)
                     /gene="rlmD"
                     /locus_tag="ACHKNB_RS00045"
                     /old_locus_tag="pgaptmp_000009"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGU4193600.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9484..12240
                     /gene="barA"
                     /locus_tag="ACHKNB_RS00050"
                     /old_locus_tag="pgaptmp_000010"
     CDS             9484..12240
                     /gene="barA"
                     /locus_tag="ACHKNB_RS00050"
                     /old_locus_tag="pgaptmp_000010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HGU4193601.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12472..13812)
                     /gene="gudD"
                     /locus_tag="ACHKNB_RS00055"
                     /old_locus_tag="pgaptmp_000011"
     CDS             complement(12472..13812)
                     /gene="gudD"
                     /locus_tag="ACHKNB_RS00055"
                     /old_locus_tag="pgaptmp_000011"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HGU4193602.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQHRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13833..15173)
                     /gene="gudX"
                     /locus_tag="ACHKNB_RS00060"
                     /old_locus_tag="pgaptmp_000012"
     CDS             complement(13833..15173)
                     /gene="gudX"
                     /locus_tag="ACHKNB_RS00060"
                     /old_locus_tag="pgaptmp_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HGU4193603.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15175..16527)
                     /gene="gudP"
                     /locus_tag="ACHKNB_RS00065"
                     /old_locus_tag="pgaptmp_000013"
     CDS             complement(15175..16527)
                     /gene="gudP"
                     /locus_tag="ACHKNB_RS00065"
                     /old_locus_tag="pgaptmp_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HGU4193604.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16962..17411)
                     /gene="yqcA"
                     /locus_tag="ACHKNB_RS00070"
                     /old_locus_tag="pgaptmp_000014"
     CDS             complement(16962..17411)
                     /gene="yqcA"
                     /locus_tag="ACHKNB_RS00070"
                     /old_locus_tag="pgaptmp_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGU4193605.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17429..18211)
                     /gene="truC"
                     /locus_tag="ACHKNB_RS00075"
                     /old_locus_tag="pgaptmp_000015"
     CDS             complement(17429..18211)
                     /gene="truC"
                     /locus_tag="ACHKNB_RS00075"
                     /old_locus_tag="pgaptmp_000015"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HGU4193606.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18211..18540)
                     /gene="yqcC"
                     /locus_tag="ACHKNB_RS00080"
                     /old_locus_tag="pgaptmp_000016"
     CDS             complement(18211..18540)
                     /gene="yqcC"
                     /locus_tag="ACHKNB_RS00080"
                     /old_locus_tag="pgaptmp_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HGU4193607.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19162..19707)
                     /gene="syd"
                     /locus_tag="ACHKNB_RS00085"
                     /old_locus_tag="pgaptmp_000017"
     CDS             complement(19162..19707)
                     /gene="syd"
                     /locus_tag="ACHKNB_RS00085"
                     /old_locus_tag="pgaptmp_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HGU4193608.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19775..20623
                     /gene="queF"
                     /locus_tag="ACHKNB_RS00090"
                     /old_locus_tag="pgaptmp_000018"
     CDS             19775..20623
                     /gene="queF"
                     /locus_tag="ACHKNB_RS00090"
                     /old_locus_tag="pgaptmp_000018"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HGU4193609.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20735..22099
                     /gene="ppnN"
                     /locus_tag="ACHKNB_RS00095"
                     /old_locus_tag="pgaptmp_000019"
     CDS             20735..22099
                     /gene="ppnN"
                     /locus_tag="ACHKNB_RS00095"
                     /old_locus_tag="pgaptmp_000019"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HGU4193610.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22656..23945
                     /gene="sdaC"
                     /locus_tag="ACHKNB_RS00100"
                     /old_locus_tag="pgaptmp_000020"
     CDS             22656..23945
                     /gene="sdaC"
                     /locus_tag="ACHKNB_RS00100"
                     /old_locus_tag="pgaptmp_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HGU4193611.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            24003..25370
                     /gene="sdaB"
                     /locus_tag="ACHKNB_RS00105"
                     /old_locus_tag="pgaptmp_000021"
     CDS             24003..25370
                     /gene="sdaB"
                     /locus_tag="ACHKNB_RS00105"
                     /old_locus_tag="pgaptmp_000021"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HGU4193612.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25482..26237
                     /gene="xni"
                     /locus_tag="ACHKNB_RS00110"
                     /old_locus_tag="pgaptmp_000022"
     CDS             25482..26237
                     /gene="xni"
                     /locus_tag="ACHKNB_RS00110"
                     /old_locus_tag="pgaptmp_000022"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HGU4193613.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26292..27440)
                     /gene="fucO"
                     /locus_tag="ACHKNB_RS00115"
                     /old_locus_tag="pgaptmp_000023"
     CDS             complement(26292..27440)
                     /gene="fucO"
                     /locus_tag="ACHKNB_RS00115"
                     /old_locus_tag="pgaptmp_000023"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HGU4193614.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27468..28115)
                     /gene="fucA"
                     /locus_tag="ACHKNB_RS00120"
                     /old_locus_tag="pgaptmp_000024"
     CDS             complement(27468..28115)
                     /gene="fucA"
                     /locus_tag="ACHKNB_RS00120"
                     /old_locus_tag="pgaptmp_000024"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGU4193615.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28662..29978
                     /gene="fucP"
                     /locus_tag="ACHKNB_RS00125"
                     /old_locus_tag="pgaptmp_000025"
     CDS             28662..29978
                     /gene="fucP"
                     /locus_tag="ACHKNB_RS00125"
                     /old_locus_tag="pgaptmp_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HGU4193616.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            30011..31786
                     /gene="fucI"
                     /locus_tag="ACHKNB_RS00130"
                     /old_locus_tag="pgaptmp_000026"
     CDS             30011..31786
                     /gene="fucI"
                     /locus_tag="ACHKNB_RS00130"
                     /old_locus_tag="pgaptmp_000026"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGU4193617.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31895..33313
                     /gene="fucK"
                     /locus_tag="ACHKNB_RS00135"
                     /old_locus_tag="pgaptmp_000027"
     CDS             31895..33313
                     /gene="fucK"
                     /locus_tag="ACHKNB_RS00135"
                     /old_locus_tag="pgaptmp_000027"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HGU4193618.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33315..33737
                     /gene="fucU"
                     /locus_tag="ACHKNB_RS00140"
                     /old_locus_tag="pgaptmp_000028"
     CDS             33315..33737
                     /gene="fucU"
                     /locus_tag="ACHKNB_RS00140"
                     /old_locus_tag="pgaptmp_000028"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HGU4193619.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33795..34526
                     /gene="fucR"
                     /locus_tag="ACHKNB_RS00145"
                     /old_locus_tag="pgaptmp_000029"
     CDS             33795..34526
                     /gene="fucR"
                     /locus_tag="ACHKNB_RS00145"
                     /old_locus_tag="pgaptmp_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HGU4193620.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34570..35670)
                     /gene="rlmM"
                     /locus_tag="ACHKNB_RS00150"
                     /old_locus_tag="pgaptmp_000030"
     CDS             complement(34570..35670)
                     /gene="rlmM"
                     /locus_tag="ACHKNB_RS00150"
                     /old_locus_tag="pgaptmp_000030"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HGU4193621.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35663..36058)
                     /gene="ygdD"
                     /locus_tag="ACHKNB_RS00155"
                     /old_locus_tag="pgaptmp_000031"
     CDS             complement(35663..36058)
                     /gene="ygdD"
                     /locus_tag="ACHKNB_RS00155"
                     /old_locus_tag="pgaptmp_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HGU4193622.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(36077..36994)
                     /gene="gcvA"
                     /locus_tag="ACHKNB_RS00160"
                     /old_locus_tag="pgaptmp_000032"
     CDS             complement(36077..36994)
                     /gene="gcvA"
                     /locus_tag="ACHKNB_RS00160"
                     /old_locus_tag="pgaptmp_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HGU4193623.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37345..37572)
                     /gene="ygdI"
                     /locus_tag="ACHKNB_RS00165"
                     /old_locus_tag="pgaptmp_000033"
     CDS             complement(37345..37572)
                     /gene="ygdI"
                     /locus_tag="ACHKNB_RS00165"
                     /old_locus_tag="pgaptmp_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HGU4193624.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37764..38969
                     /gene="csdA"
                     /locus_tag="ACHKNB_RS00170"
                     /old_locus_tag="pgaptmp_000034"
     CDS             37764..38969
                     /gene="csdA"
                     /locus_tag="ACHKNB_RS00170"
                     /old_locus_tag="pgaptmp_000034"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HGU4193625.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38969..39412
                     /gene="csdE"
                     /locus_tag="ACHKNB_RS00175"
                     /old_locus_tag="pgaptmp_000035"
     CDS             38969..39412
                     /gene="csdE"
                     /locus_tag="ACHKNB_RS00175"
                     /old_locus_tag="pgaptmp_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HGU4193626.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(39463..40269)
                     /gene="tcdA"
                     /locus_tag="ACHKNB_RS00180"
                     /old_locus_tag="pgaptmp_000036"
     CDS             complement(39463..40269)
                     /gene="tcdA"
                     /locus_tag="ACHKNB_RS00180"
                     /old_locus_tag="pgaptmp_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HGU4193627.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40346..41443)
                     /gene="mltA"
                     /locus_tag="ACHKNB_RS00185"
                     /old_locus_tag="pgaptmp_000037"
     CDS             complement(40346..41443)
                     /gene="mltA"
                     /locus_tag="ACHKNB_RS00185"
                     /old_locus_tag="pgaptmp_000037"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HGU4193628.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DTWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41652..41728
                     /locus_tag="ACHKNB_RS00190"
                     /old_locus_tag="pgaptmp_000038"
     tRNA            41652..41728
                     /locus_tag="ACHKNB_RS00190"
                     /old_locus_tag="pgaptmp_000038"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41686..41688,aa:Met,seq:cat)
     gene            complement(41803..43056)
                     /gene="amiC"
                     /locus_tag="ACHKNB_RS00195"
                     /old_locus_tag="pgaptmp_000039"
     CDS             complement(41803..43056)
                     /gene="amiC"
                     /locus_tag="ACHKNB_RS00195"
                     /old_locus_tag="pgaptmp_000039"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HGU4193629.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            43288..44619
                     /gene="argA"
                     /locus_tag="ACHKNB_RS00200"
                     /old_locus_tag="pgaptmp_000040"
     CDS             43288..44619
                     /gene="argA"
                     /locus_tag="ACHKNB_RS00200"
                     /old_locus_tag="pgaptmp_000040"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HGU4193630.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(44681..46507)
                     /gene="recD"
                     /locus_tag="ACHKNB_RS00205"
                     /old_locus_tag="pgaptmp_000041"
     CDS             complement(44681..46507)
                     /gene="recD"
                     /locus_tag="ACHKNB_RS00205"
                     /old_locus_tag="pgaptmp_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HGU4193631.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(46507..50049)
                     /gene="recB"
                     /locus_tag="ACHKNB_RS00210"
                     /old_locus_tag="pgaptmp_000042"
     CDS             complement(46507..50049)
                     /gene="recB"
                     /locus_tag="ACHKNB_RS00210"
                     /old_locus_tag="pgaptmp_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HGU4193632.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(50042..52930)
                     /gene="ptrA"
                     /locus_tag="ACHKNB_RS00215"
                     /old_locus_tag="pgaptmp_000043"
     CDS             complement(50042..52930)
                     /gene="ptrA"
                     /locus_tag="ACHKNB_RS00215"
                     /old_locus_tag="pgaptmp_000043"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HGU4193633.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(53106..56474)
                     /gene="recC"
                     /locus_tag="ACHKNB_RS00220"
                     /old_locus_tag="pgaptmp_000044"
     CDS             complement(53106..56474)
                     /gene="recC"
                     /locus_tag="ACHKNB_RS00220"
                     /old_locus_tag="pgaptmp_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HGU4193634.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(56487..56810)
                     /gene="ppdC"
                     /locus_tag="ACHKNB_RS00225"
                     /old_locus_tag="pgaptmp_000045"
     CDS             complement(56487..56810)
                     /gene="ppdC"
                     /locus_tag="ACHKNB_RS00225"
                     /old_locus_tag="pgaptmp_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HGU4193635.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56795..57202)
                     /gene="ygdB"
                     /locus_tag="ACHKNB_RS00230"
                     /old_locus_tag="pgaptmp_000046"
     CDS             complement(56795..57202)
                     /gene="ygdB"
                     /locus_tag="ACHKNB_RS00230"
                     /old_locus_tag="pgaptmp_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HGU4193636.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(57199..57762)
                     /gene="ppdB"
                     /locus_tag="ACHKNB_RS00235"
                     /old_locus_tag="pgaptmp_000047"
     CDS             complement(57199..57762)
                     /gene="ppdB"
                     /locus_tag="ACHKNB_RS00235"
                     /old_locus_tag="pgaptmp_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4193637.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVHQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(57753..58223)
                     /gene="ppdA"
                     /locus_tag="ACHKNB_RS00240"
                     /old_locus_tag="pgaptmp_000048"
     CDS             complement(57753..58223)
                     /gene="ppdA"
                     /locus_tag="ACHKNB_RS00240"
                     /old_locus_tag="pgaptmp_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HGU4193638.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(58407..59201)
                     /gene="thyA"
                     /locus_tag="ACHKNB_RS00245"
                     /old_locus_tag="pgaptmp_000049"
     CDS             complement(58407..59201)
                     /gene="thyA"
                     /locus_tag="ACHKNB_RS00245"
                     /old_locus_tag="pgaptmp_000049"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGU4193639.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(59208..60083)
                     /gene="lgt"
                     /locus_tag="ACHKNB_RS00250"
                     /old_locus_tag="pgaptmp_000050"
     CDS             complement(59208..60083)
                     /gene="lgt"
                     /locus_tag="ACHKNB_RS00250"
                     /old_locus_tag="pgaptmp_000050"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGU4193640.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(60234..62480)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS00255"
                     /old_locus_tag="pgaptmp_000051"
     CDS             complement(60234..62480)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS00255"
                     /old_locus_tag="pgaptmp_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4193641.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(62493..63023)
                     /gene="rppH"
                     /locus_tag="ACHKNB_RS00260"
                     /old_locus_tag="pgaptmp_000052"
     CDS             complement(62493..63023)
                     /gene="rppH"
                     /locus_tag="ACHKNB_RS00260"
                     /old_locus_tag="pgaptmp_000052"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HGU4193642.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(63376..63522)
                     /gene="ygdT"
                     /locus_tag="ACHKNB_RS00265"
                     /old_locus_tag="pgaptmp_000053"
     CDS             complement(63376..63522)
                     /gene="ygdT"
                     /locus_tag="ACHKNB_RS00265"
                     /old_locus_tag="pgaptmp_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HGU4193643.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            63708..64397
                     /gene="mutH"
                     /locus_tag="ACHKNB_RS00270"
                     /old_locus_tag="pgaptmp_000054"
     CDS             63708..64397
                     /gene="mutH"
                     /locus_tag="ACHKNB_RS00270"
                     /old_locus_tag="pgaptmp_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HGU4193644.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            64466..65179
                     /gene="ygdQ"
                     /locus_tag="ACHKNB_RS00275"
                     /old_locus_tag="pgaptmp_000055"
     CDS             64466..65179
                     /gene="ygdQ"
                     /locus_tag="ACHKNB_RS00275"
                     /old_locus_tag="pgaptmp_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4193645.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            65317..65535
                     /gene="ygdR"
                     /locus_tag="ACHKNB_RS00280"
                     /old_locus_tag="pgaptmp_000056"
     CDS             65317..65535
                     /gene="ygdR"
                     /locus_tag="ACHKNB_RS00280"
                     /old_locus_tag="pgaptmp_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HGU4193646.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            65643..66683
                     /gene="tas"
                     /locus_tag="ACHKNB_RS00285"
                     /old_locus_tag="pgaptmp_000057"
     CDS             65643..66683
                     /gene="tas"
                     /locus_tag="ACHKNB_RS00285"
                     /old_locus_tag="pgaptmp_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HGU4193647.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(66715..67908)
                     /gene="lplT"
                     /locus_tag="ACHKNB_RS00290"
                     /old_locus_tag="pgaptmp_000058"
     CDS             complement(66715..67908)
                     /gene="lplT"
                     /locus_tag="ACHKNB_RS00290"
                     /old_locus_tag="pgaptmp_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HGU4193648.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(67901..70060)
                     /gene="aas"
                     /locus_tag="ACHKNB_RS00295"
                     /old_locus_tag="pgaptmp_000059"
     CDS             complement(67901..70060)
                     /gene="aas"
                     /locus_tag="ACHKNB_RS00295"
                     /old_locus_tag="pgaptmp_000059"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HGU4193649.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            70646..71677
                     /gene="galR"
                     /locus_tag="ACHKNB_RS00300"
                     /old_locus_tag="pgaptmp_000060"
     CDS             70646..71677
                     /gene="galR"
                     /locus_tag="ACHKNB_RS00300"
                     /old_locus_tag="pgaptmp_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HGU4193650.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(71684..72946)
                     /gene="lysA"
                     /locus_tag="ACHKNB_RS00305"
                     /old_locus_tag="pgaptmp_000061"
     CDS             complement(71684..72946)
                     /gene="lysA"
                     /locus_tag="ACHKNB_RS00305"
                     /old_locus_tag="pgaptmp_000061"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGU4193651.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            73068..74003
                     /gene="lysR"
                     /locus_tag="ACHKNB_RS00310"
                     /old_locus_tag="pgaptmp_000062"
     CDS             73068..74003
                     /gene="lysR"
                     /locus_tag="ACHKNB_RS00310"
                     /old_locus_tag="pgaptmp_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HGU4193652.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(73990..74682)
                     /gene="ygeA"
                     /locus_tag="ACHKNB_RS00315"
                     /old_locus_tag="pgaptmp_000063"
     CDS             complement(73990..74682)
                     /gene="ygeA"
                     /locus_tag="ACHKNB_RS00315"
                     /old_locus_tag="pgaptmp_000063"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HGU4193653.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(74811..76229)
                     /gene="araE"
                     /locus_tag="ACHKNB_RS00320"
                     /old_locus_tag="pgaptmp_000064"
     CDS             complement(74811..76229)
                     /gene="araE"
                     /locus_tag="ACHKNB_RS00320"
                     /old_locus_tag="pgaptmp_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HGU4193654.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(76544..77305)
                     /gene="kduD"
                     /locus_tag="ACHKNB_RS00325"
                     /old_locus_tag="pgaptmp_000065"
     CDS             complement(76544..77305)
                     /gene="kduD"
                     /locus_tag="ACHKNB_RS00325"
                     /old_locus_tag="pgaptmp_000065"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HGU4193655.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(77335..78171)
                     /gene="kduI"
                     /locus_tag="ACHKNB_RS00330"
                     /old_locus_tag="pgaptmp_000066"
     CDS             complement(77335..78171)
                     /gene="kduI"
                     /locus_tag="ACHKNB_RS00330"
                     /old_locus_tag="pgaptmp_000066"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HGU4193656.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(78458..79639)
                     /gene="yqeF"
                     /locus_tag="ACHKNB_RS00335"
                     /old_locus_tag="pgaptmp_000067"
     CDS             complement(78458..79639)
                     /gene="yqeF"
                     /locus_tag="ACHKNB_RS00335"
                     /old_locus_tag="pgaptmp_000067"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HGU4193657.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            79894..81123
                     /gene="yqeG"
                     /locus_tag="ACHKNB_RS00340"
                     /old_locus_tag="pgaptmp_000068"
     CDS             79894..81123
                     /gene="yqeG"
                     /locus_tag="ACHKNB_RS00340"
                     /old_locus_tag="pgaptmp_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4193658.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        19952 a        21793 c        19724 g        19756 t
ORIGIN      
        1 agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtgtt
       61 agtttttaat gataacgaat ctaaaattac cgatgccgcc gtcaatttat ttatccagat
      121 ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgtg tgttcctttg
      181 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacggat cttacgagta
      241 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agactctatt ttcaataata
      301 cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca attgaacaat
      361 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      421 tgatcattgt tgcctggtca gatcgtactg tccgcatcca ggtaggttat gggctggaag
      481 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      541 aacaacaaaa atttgctcag ggattagagc tagctataaa cgctttgaat aaccaactca
      601 cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct tccagtgatc
      661 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      721 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctatatttca gcaatctatc
      781 ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt tccttctttt
      841 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatcag
      901 gtaaaagtta tcattcagac aacagcggtt ccggtggagg ctcaagtggc ggcggattta
      961 gcggcggtgg cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa ctcaaccata
     1021 aaaaatgcca gcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc
     1081 ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa
     1141 tcagctggtt gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc
     1201 ctgcagcagt accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt
     1261 gagagataac tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg
     1321 tcagagaacc gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac
     1381 cttctttcag gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt
     1441 cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt
     1501 cgatagaaac gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag
     1561 aggtgaacgc tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag
     1621 aagctgcgca gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa
     1681 cagcttcagc gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac
     1741 cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga
     1801 aaacttcaga acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca
     1861 tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca
     1921 tcggaaccgg catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca
     1981 gcggcatacc tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg
     2041 cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa
     2101 tgccagcctg atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg
     2161 cagcaacagc tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt
     2221 ccagagcttc acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac
     2281 caccctccag atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac
     2341 gaccgatgat ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa
     2401 actccagaca aacaacgcgt acccagggta cgcgttgccg ttctaacttt tttacttact
     2461 tcgcctgacg cttctggaac tcgctggcgg cttttacaaa gccagcaaac agcgggtgac
     2521 catcacgtgg agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat
     2581 tcggaacttc gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac
     2641 ctgcatcttc aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct
     2701 caacaattgt cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact
     2761 gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt
     2821 tgccgttttc atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca
     2881 caaattccgt agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg
     2941 ccacctgcat acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg
     3001 cggtcgtaat catgccttct acgccacgat agccaaaacc gccaggtact aggattgcgt
     3061 ccagaccttt aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga
     3121 tgttgacgct gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat
     3181 aagcatccgg cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt
     3241 tcgcttcttc gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc
     3301 tgaatcgttt acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat
     3361 agatggaatc gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg
     3421 caatcttcgc acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag
     3481 gctggatacc gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt
     3541 caccagacgc tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc
     3601 caatttcaac agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta
     3661 ctgtaccgcc gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct
     3721 ctttgattgc gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc
     3781 ggcgttcttt acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc
     3841 tcattttggt acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt
     3901 cttcagtaac gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga
     3961 tgtacggatc cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga
     4021 gggaggctgc ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat
     4081 agttcgttgt catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac
     4141 gggaaagcag tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg
     4201 ccagcgcact attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac
     4261 gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc
     4321 ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc
     4381 aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg
     4441 ctcgaatttt tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc
     4501 cagattaacc gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac
     4561 aacagcctgc cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcgactac
     4621 cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc
     4681 acgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg
     4741 cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc
     4801 agaactatca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca
     4861 aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg
     4921 gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc
     4981 cagcacttcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt
     5041 atcccacggg cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc
     5101 gatttgattc attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca
     5161 gaatagaagt gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt
     5221 gtaattcctc tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgatac
     5281 tttttacctg atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga
     5341 acggatatcc ttttgattgc gttgtataag gaacacacag acacatacct gttttgttgt
     5401 tgtacatgaa aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg
     5461 tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta
     5521 ccagacttcc ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt
     5581 gatgtcgttg accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat
     5641 taatttgcca tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg
     5701 cattaacgta gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg
     5761 gatcataacc ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat
     5821 tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc gacgcgcgtc gataacatcc
     5881 ggcacctggt tgagtttacc cagcacgcgc cccagcactt gcaggttgta aatctcaatg
     5941 gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg
     6001 ttcaccttct cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta
     6061 gccaccacac ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca
     6121 acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt
     6181 actgaaatac cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg
     6241 cagcagcgcg cgatgtggtg catcaggttg ccaacacctt cgaccaccac gcgaccgtta
     6301 tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg
     6361 tcctgctctt cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg
     6421 agacggatat ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa
     6481 cgcggcagca gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg
     6541 tcaaggattt gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg
     6601 tgaattttcg aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca
     6661 cggctggggt tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg
     6721 taggtgaacg gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca
     6781 ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca
     6841 cctttcggcg taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc
     6901 atttcgccgg aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc
     6961 cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc
     7021 gcagcaacac ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg
     7081 gttttgccac ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg
     7141 ttagcgacat aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc
     7201 agtgcggcat aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac
     7261 agctcatcga aggcgaggtt ctttttctgc attttgcgcc agatactgta gatgtgtttc
     7321 ggacgaccat acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgacca
     7381 acgaactctt cgatgtagtg ttcgcggtcg agacgccgtt catgcagcag tttggcaatt
     7441 cgtttgtatt cggttggatg gaggtaacgg aagcagtaat cttccagttc ccatttcagt
     7501 tgtccgattc cgagacggtt agccagcggt gcgtagatgt tggtacactc ttttgccgcc
     7561 agtacacgtt catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc
     7621 agtttgatga ctacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta
     7681 tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc
     7741 atatcacgca cgccgtgaat aaggttaacg accgacttac cgacgctctc acgcagcaca
     7801 tcttcgctga ctacgttggc atcagccaga gggaacagca gcgccgcccg cagcgtgtca
     7861 atgtccatac ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga
     7921 ctggcatccg gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac
     7981 tcacacgact tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca
     8041 ccagccttat tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag
     8101 acctgccgaa atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca
     8161 gatgtcccgt gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt
     8221 ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac
     8281 gaataggttc cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt
     8341 ccagcaacac tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa
     8401 gattttcgtg ataaaacgtc acattctgta aaccattaag acgcgcattc tgctggcctt
     8461 tttccaccag cgccggaaca ccttctacac cgaccacact ggctgcttgt ttcgccaatg
     8521 gcagtgtaaa gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat
     8581 ccagccattc caacgcacgc gctaccattt tttggttcac acccgcattg acctgaataa
     8641 aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc atctcaccag
     8701 agacggtttc gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg
     8761 aaaagcgttc cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca
     8821 tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc aagatggcgc ataacttgta
     8881 agctgcccag gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg
     8941 ggcattgttt aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt
     9001 gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg gcggcgatag ccccagggaa
     9061 catcggcgat cacttcagag acatcgtgtt tcattaatcg ggcgagtgcc gcacttttgc
     9121 ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat
     9181 gaggacagcg tggtgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc
     9241 gggcatactg ttttttatct tcagtaacag caacttccgc gttttcctgc ggcaataatc
     9301 cggggataaa taacgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt
     9361 cgttgactga aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt
     9421 gcgccattgg cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac
     9481 tccatgacca actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc
     9541 cttattggtt tattgctgag tatctttttc gtcgtgcatc gctataacga cttgcagcgt
     9601 caactggaag atgccggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc
     9661 atgagcctgc aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat
     9721 tccgatattg ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc
     9781 aattttcatc ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag
     9841 ctcactgtca ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt
     9901 tactcccccg acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat
     9961 attgcgctgg agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt
    10021 tccagcgtga tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg
    10081 cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg
    10141 caactcgaca gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt
    10201 atcaactcga tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac
    10261 caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagtta
    10321 gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat
    10381 atgtcacacg agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg
    10441 aaaacagaat taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat
    10501 aatttgctgg caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg
    10561 attctggaaa gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg
    10621 gcacattctt ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct
    10681 gataacgtga tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat
    10741 gcaattaaat tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt
    10801 aataccaaag tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc
    10861 gatcaatcgc gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat
    10921 ggtggcaccg gtctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat
    10981 atttcgttcc atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat
    11041 ctgaacccga acattattat cgaagggcca tccacccagt gcctcgccgg taagcgcctg
    11101 gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg
    11161 ccgctggaag tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg
    11221 atgttgttag gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta
    11281 gcgaaagcgg tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc
    11341 aatgcagaaa aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct
    11401 acgcgcctgt tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct
    11461 gtaaccgatg aaagcaagct ggcaatgaca gtcatggcag ttgatgacaa ccccgctaac
    11521 ctgaaactta tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc
    11581 gggcatcagg cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt
    11641 caaatgcctg acatggatgg cattcgggcc tgcgagctca tccaccagct cccccatcag
    11701 caacaaacgc cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg
    11761 cttggcgcag ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat
    11821 ttgttgttgc gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc
    11881 aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca
    11941 gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa
    12001 gttcgcaaca aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg
    12061 attcataaac tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc
    12121 caactgatcg aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg
    12181 ctggaactgt tggacgagat ggataatgta gcgcgcgaag ccagcaaaat tctcgggtaa
    12241 cggacgtctt gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa
    12301 gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa
    12361 attcaatgaa ttaagaaatg atgtagacct gatacatagc acaacaggct attttgcgtt
    12421 tagcatcagt ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac
    12481 catgcacggg cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc
    12541 atcgtcacgc gcgccaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc
    12601 catatcgatt tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat
    12661 ctcaaacggt tctttggtca ggcgctgatt accttcctgc cagatccagt gcgtatcaat
    12721 agcagtaatt ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc
    12781 gaagtggttg tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac
    12841 acgtaccgaa ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg
    12901 cagggagagc gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg
    12961 caggccagtt gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc
    13021 accacacgga tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc
    13081 ttcgttcagc gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg
    13141 cgccagtgcc acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt
    13201 gaaatcgttg aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg
    13261 cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg
    13321 atacggcagc ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac
    13381 ttcgctacga tgttgaccat cgcccagcag cgatgccacg tttaccccca gatgctgccc
    13441 cagcagatcc agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc
    13501 aaatgtctgc aaaccgcgcc cgccggcatc acgatcggca aatgtattac gcaccagcgt
    13561 cagaacgttt ttgtattcac ccagcgttct acccaccacc agcggaatcg catcttccag
    13621 cgttttacgg attttctcgc cgccgggaat ttcgcctacg ccggtgtgac cggaattatc
    13681 tttgataatc acaatattac gcgtgaagaa cggtgcgtgt gcaccactca gattcatcag
    13741 catactgtca tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa
    13801 ttgagaactc atatttatgt ccttattcag aatcagtgac ggccgaaaac gggacgttta
    13861 cggtcaaagg tccagccggg gatcagatac tgcatcggac ctgcgtcgtt acgcgcaccg
    13921 ccaggcagac gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg
    13981 cccagaccgg gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg
    14041 gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc
    14101 ggtgccgccg cgcccacatg ggtaaacatc gccagagaaa tatcgaaatg gttattagaa
    14161 tggcagcccc aggttagccc ccagtcgtcg caaagctgtg ccacgcggac tgcaccggaa
    14221 agcgtccaga agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatgg
    14281 cccatttcgc gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgt
    14341 cgaaactccg ccatcacttc acgaccggag aaaccctgct cagcgccgca cggatcttcg
    14401 gcataggtaa gaacatcatt cagccctttg cacaaagaaa tggcttcatc aagcagccat
    14461 gcaccgttgg gatcaacggt aatccgcgca tccgggaagc gtttcttcaa tgcacgaaca
    14521 gtgtcgattt cttgctcgcc aggtaacact ccgcccttaa gtttgaaatc tttaaagcca
    14581 tagcgatcct gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga
    14641 tggcgcaact gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt
    14701 ttggtccgat caccgatata aaacagataa ccgaggacgg taatagcatc gcgttgcttg
    14761 cctggcccta acagttcgca gaccggaaca ttcaacgcct tacctagcag gtcaagcaag
    14821 gcggcttcca gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg
    14881 aaggtatcaa aatcggccgc ctgattacct atatgcacct gctgaaccac tttattcagg
    14941 cgcgcaactt cctggcccag caccatcgga atagcatcga caagcgtctg ataaatcacc
    15001 tctccgcccg gcgcttcacc aataccggta tgcccggcat tatcggtgag taccacaata
    15061 ttgcgggtga aatatgcgct atgtgcacca ccaatattaa gcaacatgct atcgtgcccg
    15121 gcgaccggaa tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg
    15181 ccccgcgaca ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag
    15241 taccgcgatt aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt
    15301 gccaacgata taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt
    15361 gaacaggcca ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca
    15421 acccagcgcc ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca
    15481 ctcaacgttg acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat
    15541 cggtgtttta cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat
    15601 cccacccagc acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc
    15661 tttcagaatc gacatcccgc gtgcctgcac cagataaacc gggaaccagg taataaagaa
    15721 gtaagtcagg gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc
    15781 gagcagctgt ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg
    15841 ctgatccata ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac
    15901 ccccggatgt tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc
    15961 cagaccgccc ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat
    16021 aatcggcgcg aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt
    16081 tcccctttcc tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg
    16141 cgcttcagca agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc
    16201 actaaagata tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca
    16261 gaagtagacg cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat
    16321 aacataagcc catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat
    16381 atctttggcc atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga
    16441 tgtgacgata aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact
    16501 gctcgcagcc tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc
    16561 tcactctgga ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt
    16621 tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag
    16681 cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt
    16741 ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag
    16801 gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa
    16861 cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga aaatattcga
    16921 ttcagcaccg ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc
    16981 cactgttcga cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc
    17041 aacagcattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg
    17101 ccgccattgc agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga
    17161 ttcggctgga agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga
    17221 aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg atagggcagc
    17281 cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt
    17341 gcttctgctt cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca
    17401 atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg
    17461 gcgcactgaa ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata
    17521 acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag
    17581 gatgcgtcaa tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag
    17641 caccgctgcg attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca
    17701 aatgggcaag atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca
    17761 ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac
    17821 cgcgataatg cgtcactgct ggctgcgagc ctttatcttc gcgggcaaat ttatcagcga
    17881 ttttgtccag ttcttccacc agtggataat ccagaactgc ttcttccatc aaccagccgc
    17941 gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc
    18001 gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat
    18061 gagcggtaaa aacgtgctgg ccaatctgat cacgcacggt ttgcatgacc actactttct
    18121 cgtcgcgatc cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca
    18181 gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc
    18241 cagtttttcc agctcagcca gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat
    18301 ttcataatag ggtgcaacgg caaaagcgcc cggtaacggc tgtttgttat ctaacaaatc
    18361 gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa
    18421 gaacggttgg gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg
    18481 ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat
    18541 aaagcaacct caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac
    18601 tgtattcaca gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc
    18661 tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt
    18721 cctgctcaca ccaccccgat gtgatacttc atcctgaagt gtccctggcc ttcctgaccc
    18781 accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg
    18841 cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg
    18901 cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga
    18961 atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca
    19021 tatgaaagtt aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat
    19081 ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca
    19141 caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag
    19201 atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga
    19261 gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg
    19321 cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg
    19381 gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg
    19441 catatccccg gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc
    19501 aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta
    19561 tacggcatct tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact
    19621 taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt
    19681 cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt
    19741 gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc
    19801 acttgcgggc ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccaacct
    19861 actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct
    19921 gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg
    19981 tttgccgcag gttgccgtcg gtcacgttga acttgattac accagcgcaa atctgattga
    20041 gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga
    20101 tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt
    20161 ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttca atggcacctg
    20221 cattgatgac caggatatca ctatcgataa ctatgaattc actactgact atctggagaa
    20281 tgccaccagc ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa
    20341 ctgcctgatc acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca
    20401 aattgacaga gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca
    20461 cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc cagagaaatt
    20521 aagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa
    20581 tagcgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg
    20641 cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag
    20701 ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg
    20761 ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga
    20821 cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga
    20881 taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc tgcgccgtga
    20941 acgcggcgta aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat
    21001 tattcgcgct ttgcaggcca acttgttcgc ggttctgcga gacattctct tcgtttacgg
    21061 gcaaatccat aataccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac
    21121 taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa
    21181 tatggtggtc tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg
    21241 cgtcggaaac cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc
    21301 gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag
    21361 tcgttttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt
    21421 caacgaattg atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc
    21481 tcacggtatc attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct
    21541 gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc
    21601 gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtacata cgctgggtga
    21661 aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat
    21721 gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgtgat acaggcgatg cctacagctt
    21781 taactggtca atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa
    21841 tatggctaat ctgaagcttt acccggatca acctgttgaa gtgctggctg ccgacctgcg
    21901 ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga
    21961 agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct
    22021 gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg
    22081 ctacgaaatc tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt
    22141 ttttggtcac ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc
    22201 acgcgacaaa tatcatcaca gttaatatgt catacaattt atattgcaac gcaaacgttt
    22261 ccctattttc ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact
    22321 aaaagtattg attttttcag ttcaacctac atatattgcg cgccccggaa gaagtcagat
    22381 gtcgtttaat gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa
    22441 gcgttgggat aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag
    22501 atacataact tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc
    22561 atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa
    22621 aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga
    22681 ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc
    22741 tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg
    22801 gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc
    22861 gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg
    22921 tagaagaaca ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta
    22981 tctacccgat cctgctggtt tatagcgtgg caatcaccaa taccgttgaa agcttcatgt
    23041 ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg
    23101 gtatgatgac catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg
    23161 tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctcatcccg cagtggaacg
    23221 gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga
    23281 tgaccctgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct
    23341 cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga
    23401 agatccttgc attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct
    23461 gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc
    23521 tgtcttacct ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg
    23581 cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca
    23641 acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga
    23701 accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga
    23761 gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga
    23821 tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca
    23881 acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca
    23941 gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc
    24001 ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg
    24061 ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt aatctgctta
    24121 aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc
    24181 accacactga tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata
    24241 tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa
    24301 acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat gcagacaacc
    24361 tttctctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca
    24421 gccagactta ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc
    24481 agaatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac
    24541 attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaatgag ctggcgctgc
    24601 acagcaaaga agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta
    24661 ttgagcgcgg tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg
    24721 ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg
    24781 ttgttgactg gatcaacatg tttgcattgg cagtgaacga agagaacgct gctggcggac
    24841 gcgtggtgac tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact
    24901 acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca
    24961 gtgccattgg ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc
    25021 agggtgaagt tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg
    25081 caagcccggc gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga
    25141 cgtgtgaccc ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg
    25201 cagtaaaagc ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct
    25261 gcctcgataa agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc
    25321 gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca
    25381 gaggcctcgt tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca
    25441 tcgaatgtta cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta
    25501 ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg
    25561 aaacctgcca gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg
    25621 ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca
    25681 aagcgggtcg accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg
    25741 cctttgagca acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag
    25801 ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag
    25861 ataaaggcta ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac
    25921 gttggctgga tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg
    25981 attactgggg acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac
    26041 caaaaagcgc cacgcagctg ctggtcgagt ttcagagtct ggaaggaata tatgagaatc
    26101 tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc
    26161 tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat
    26221 tgcggttggt acggtaacgg cgagccggat acgccgcaaa cgtcgtatcc ggcattatca
    26281 catcagcgca tttaccaggc ggtatggtaa agctctacaa tatcctcaag cgttgcttca
    26341 cgcgggttgc caccggtaca aacatcatcc agtgccgcct gcgccagtgc cggaatgtct
    26401 tccttgcgta caccaacatc acgcaaatgt ggcggaatac cgacatcacg gttgagagca
    26461 aacaccgctt caacagcggc attacgcgcc tcttccaggc tcataccttc cactttcacg
    26521 cccataacgc gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg
    26581 acatgcggta acaggatggc gttcgcaaca ccgtgtggag tgttgtaaaa cgcgcccagt
    26641 ggatgcgcca taccatgcac caaccctaac ccaacattcg agaagcccat acccgcaaca
    26701 tactgcccga gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc
    26761 gccccagcaa tgatttcaat cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca
    26821 cgggtaatat acccctcaat agcatgagtg agcgcatcga cacccgtcgc agctttcagc
    26881 gctggaggca taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc
    26941 ggatcaacgc aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta gttaatggtc
    27001 acttctgccg cagtacctgc tgtggtagga attgccagaa tcggtacact gggtttattg
    27061 gtcggggaaa gcccttccag gctacgcaca tcggcaaact ccgggttgtt gctgataatg
    27121 ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg
    27181 ccgctattct ggaatacacc gagcccttct ttgacgacag taattgttgg gttgggcact
    27241 acgccgtcgt aaatcgccca tgccagccct gcagcatcca tcttatcggt cactttcgcc
    27301 accacgccgc attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg
    27361 cgtttcacct catcggttaa agccccaaca gcaccccgac caaaccatgc cgtttcgttc
    27421 agaatcattc tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatc
    27481 cgtaacccat aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact
    27541 ggcaccgggt ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac ttcaacttca
    27601 tgcgccaacc ataacgcttt ttccagattc acctcacaag cgataagccc atgatgttgt
    27661 aacaaagttg ccttacgatt tttgagagcc tgcgcaacat gttcagaaag ttcacgtgtt
    27721 ccaaaggtcg cataaggcgc gcaaggaata gaattaccgc cagccgccgc aatcatgtag
    27781 tgaatagcgg ggatcgatcg gttaagaatg gaaactgccg tgcaatgaac ggcatgattg
    27841 tgaacaaccg cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccattcg
    27901 cttgagggga gctttccttc ctcatgttta ccgttgccat caataaagac aatatgcgac
    27961 tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca gcatcccatc ctgataacgt
    28021 acactgacgt tccccgctgt cccctggttc agtcccaggc gggtcatttc caggcaagtg
    28081 tcaataatct gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac
    28141 agggcaataa tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccatctt
    28201 cttcctgatt aaccagaaag aaattcacaa gcttatattt tgtgacctgg ttcaactaat
    28261 cacagtaaat aactgcaagt tctcttttta taaccccatt aaaaatgacc gctcttaaaa
    28321 atatttatca aaacggtcat ttttctattc actcaagcca ggaatgcccg ttttcggcat
    28381 aaacaattaa tacggtcatc tggtttgtgt tttttatgat ttattttctg aaacgggcat
    28441 gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc taataagtgt
    28501 gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag ttcatttcag
    28561 acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt ttgaaaacat
    28621 aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac acatcaatac
    28681 aaacgcagag ttaccgtgcg gtagataaag atgcagggca aagcagaagt tacattattc
    28741 cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac cttaacgaca
    28801 ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct ggcctgatcc
    28861 aatcggcctt ttactttggt tatttcatta tcccaatccc tgctgggata ttgatgaaaa
    28921 aactcagtta taaagcaggg attattaccg gattattttt atatgccttg ggcgctgcat
    28981 tattctggcc cgctgcagaa ataatgaact acaccttatt tttagttggc ctatttatta
    29041 ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg gtattagggc
    29101 cggaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg tttggcgcaa
    29161 ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat caatcgcaag
    29221 acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc ctggtattat
    29281 cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca
    29341 tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa caaggatcgt
    29401 tctccgcatc gctttctcgc ctggcgcgta ttcgccactg gcgctgggca gtattagcgc
    29461 aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc tacgctgtag
    29521 aagaaattcc aggtatgact gcaggctttg ccgctaacta tttaaccgga accatggtgt
    29581 gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag
    29641 tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg
    29701 gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt cagtacccaa
    29761 caatcttctc gctgggcatt aagaatctcg gccaggacac taaatacggt tcgtccttca
    29821 tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt gtcagtgacg
    29881 cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg gtcatcttta
    29941 tctttgcccg tttccgctct caaacggcaa ctaactgaac atattttccg aataaagtga
    30001 ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg
    30061 tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagctac
    30121 ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat
    30181 ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag
    30241 tcagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca gtgaaaccat
    30301 cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg
    30361 cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc
    30421 catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga
    30481 aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta
    30541 tctgtcgctg ggcggcgttt cgatgggtat cgccggttcc attgttgatc acaacttctt
    30601 tgaatcctgg ctgggaatga aagttcaggc ggtggatatg accgaactgc gtcgccgtat
    30661 cgatcagaag atttacgacg aagccgaact ggaaatggcg ctggcctggg ctgataaaaa
    30721 cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg agcaaagccg
    30781 cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa
    30841 cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc
    30901 gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca ccgccgaagc
    30961 gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga
    31021 aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg gcaccgctca
    31081 ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg taacggggca
    31141 taaactcgat ggactggcag aacacggcat catccatttg atcaactcag gttctgctgc
    31201 gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga agccgcactg
    31261 ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat
    31321 ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc
    31381 gttcaccatg actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga
    31441 aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc
    31501 aacctggcca accacctggt ttgcaccgcg cctcactggt aaagggccgt ttacggatgt
    31561 gtattcggta atggcgaact ggggtgccaa ccacggagtt ctgaccatcg gccacgttgg
    31621 cgcagacttt atcactcttg cctccatgct gcgtatcccg gtatgtatgc acaacgttga
    31681 agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata ttgaaggcca
    31741 ggattaccgc gcttgccaga actacggtcc gttgtacaag cgttaataca ttactccctg
    31801 atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata
    31861 aacaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact
    31921 cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa aaattgttgc
    31981 ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg
    32041 gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact
    32101 gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt
    32161 agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc
    32221 ggttatggac aatattgaac ggttaatctc cgcacagcgg ttgcaggcta tttctggcgt
    32281 cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca
    32341 actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac
    32401 cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca
    32461 acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg
    32521 tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg
    32581 cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg
    32641 cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt
    32701 ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact
    32761 ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga
    32821 atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga ttgaagaagc
    32881 tcgtctgatc gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat tgtcgtgtca
    32941 gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc
    33001 gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca
    33061 ctttaaggcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca
    33121 gattaaagcc aatatgcttg atattccggt aaaagttctc gacgacgccg aaacgaccgt
    33181 cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc cggaagaagc
    33241 ccgcgcacag attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat
    33301 agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag
    33361 tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc
    33421 attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg
    33481 cgattatccc gttatttgaa ctggacagtt atgcaccgcc gctggtgatg atggcggcgg
    33541 tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg ctttcactac
    33601 aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa
    33661 aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa
    33721 aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg
    33781 atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc
    33841 ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc
    33901 gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta
    33961 aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc
    34021 attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg
    34081 tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca
    34141 acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca
    34201 ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct
    34261 cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga
    34321 tcgatagcag cggcgcactg tgggactcca atgcgatcaa cgctgattac aaatcgatgc
    34381 tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag
    34441 gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg
    34501 ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg
    34561 ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg
    34621 caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc
    34681 atgttcatca agctgtgcct gaatatacgc cagattgtgt gacacttctt cgtagcgttt
    34741 tttcatcggc agtttgaggt tgaaaatggt ttcacggcac cagccattaa ccagccactg
    34801 cgccatcaat gccgcaactt tcgccggttt ttcaaccata tcgcatacca tccaggagat
    34861 attgctgcgc gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgtccggt
    34921 atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt
    34981 caccagttgg taggtccagc cgccagggca agcgcctaaa tccaccgccc acatcccgtt
    35041 cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt
    35101 gagcgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa
    35161 cggcgaatta ttgttgctgt atgagtaacc ggtatagcag cagcctggtg caataaagaa
    35221 tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccgg catcgcgcaa
    35281 ggcagcgcgt agcggaacag taaatttacg gcagaatttc agtaactctt tgctttcgtt
    35341 ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc
    35401 gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa
    35461 ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc
    35521 atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc
    35581 gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc
    35641 cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag
    35701 caccaactaa cattaacgcc cagcccgcga ggaagctcac gccgccaacc ggagtgacaa
    35761 acgcccacaa acgcagatgg gacagcgcca ggcaataaag gctgccgctg aacaacaccg
    35821 tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg
    35881 ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtctgga
    35941 tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca aaagcgccca
    36001 gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg
    36061 tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg
    36121 ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat
    36181 gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac
    36241 gtccggcctc gatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt
    36301 gaatagccgc ttgcagcacc atggcgctat gactaaaaat tggcccttgc tgaacgttga
    36361 tatgatttaa ccccaactgt cgggtatatg tctgccagtc acggcgtgac gcatcatgta
    36421 ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcct gtcagcagta
    36481 gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc
    36541 agttgccccg accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac
    36601 gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc
    36661 gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg
    36721 cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat
    36781 cgaggaaata gctttgccct tcctcggtca gcaggagtga acgattacgg cggcggaaca
    36841 gttttagccc caaaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca
    36901 caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc
    36961 gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga
    37021 atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa
    37081 ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg
    37141 aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt gatgttgtgt
    37201 tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact
    37261 tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc
    37321 ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt tcacgtcagt
    37381 acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac cggtatcgtt
    37441 atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata
    37501 gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc
    37561 agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta ttgattaaca
    37621 ggctaaaatt aacgcctaac actattcagc atatgttact tacgcggctt cgccaggata
    37681 tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc
    37741 ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt
    37801 ttcccgcact acaggatgcg ggcgtctatc tcgatagcgc cgcgaccgcg cttaaacctg
    37861 aagccgtggt tgaagccact cgacagtttt acagcctgag cgccggaaac gtccatcgca
    37921 gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg
    37981 cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat
    38041 ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta
    38101 ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa
    38161 ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt
    38221 tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta
    38281 ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg
    38341 tgatggttga tggtgctcag ggggcagtgc atttccccgc ggatgttcag caactggata
    38401 ttgatttcta tgctttttca ggtcacaaac tgtatggccc gacaggtatc ggcgtgctgt
    38461 atggtaaatc agaactgctg gaggcgatgt cgccctggct gggcggcggc aaaatggttc
    38521 acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg gaagctggaa
    38581 cgccaaatgt cgctggtgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg
    38641 atatcaacca ggccgaaagc tggagccgta gcttagcaac gctggcggaa gatgcgctgg
    38701 cgaaacgtcc cggctttcgt tcattccgct gccaggattc cagcctgctg gcctttgatt
    38761 ttgctggcgt tcatcatagc gatatggtga cgctgctggc ggagtacggt attgccctgc
    38821 gggccgggca gcattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc
    38881 gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg
    38941 accgcgcgct ggaattattg gtggattaat gacaaacccg caattcgccg gacatccgtt
    39001 cggcacaacc gtaaccgcag aaacgttacg caataccttc gcaccgttgt cgcaatggga
    39061 agataaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt
    39121 aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt
    39181 gactgaaaac ggtaaaatgc acttctttgg cgacagcgaa gggcgcattg tgcgcggcct
    39241 gttggcggtg ttgttgactg ccgttgaggg gaaaaccgtc gccgaattgc aggcacagtc
    39301 gccgctggca ttgtttgatg agctgggatt gcgtgcacag cttagcgcct cacgcagcca
    39361 ggggttgaat gcgttaagcg aggcgattat cgctgcggcg aagcaggttt aagcacagaa
    39421 ccaggtcgga taagacgttc gcgtcgcatc cgacctgatt gtttaaccct gacgcgccgc
    39481 tttcgccatc atcttcttca gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac
    39541 catcgttgcc gcgccaaatc cagatgcaca atccatccgc ttcggccctt ctgccgtggc
    39601 tttcatcgca cacacagtac cgtctgactg cgggtacacc agcgcttcag tagaaaacac
    39661 gcaatcgacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg
    39721 ctcgcgcaac ttcgccgcca gcggatcctg aatcgttttc gccagatcgg taacctgaat
    39781 ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg
    39841 ccgacaataa gcaatcagcg ccgctttggg ccgtacacta tcaatggcat caatcacgta
    39901 cgaataaccc acgctcatat actgcgctac gttatccggc gtcacgaaat catccaccac
    39961 cgttacacgg cactccgggt taatctggcg aatacgctcc gccatgactt ccgcttttgc
    40021 cagcccgacg ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc
    40081 atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca
    40141 ggaaccgaca ccaccgatac caaccacaca aatatgcgcg tcagcaaaca gttgcaacgc
    40201 tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac
    40261 cacagacata gcacctcaga attaaaaagg gtgaggttat ccctcaccct ggcgtatccg
    40321 taaaaacggt atacagaata acacatcagc cgctaaagac gttacctgcg cccggggcgg
    40381 ttttcagcac ccagacacgt ccatagtggt tgtaccaacc tgcgcgatgt ccggcttccg
    40441 gcccgatccc ttgatagata tcgaagtgtt ggcctttgat tgcgccaccg acatccagcg
    40501 ccaccatcag acgcaattcg tactgaccgt taaatttgcc gttattatcc agcagcggca
    40561 cttctgccag caaggtagta cctggcggaa taattgaacg atcggaggca acagaagcac
    40621 ggccaaccag cggcaccgca cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga
    40681 aaacgaaaga cgggttctgt tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc
    40741 cccaatgacg aatcgcctgc atcgacatat cttctttttt cacttcgcca cggtcgatca
    40801 gcaccttacc aatgctgcga taggcatgac cgtttttccc tgcatagctg aaaaagttaa
    40861 gcggactgcc atcaccaaag tcgatatacc cgctaccctg cacatccata atgaagttat
    40921 ccatcaggga gttgctgtaa gcgagaatat atttatcact cagcgcccct gcgtagatct
    40981 ccgcacgaga cggcagacga ccacgttttg gcggcatacg gtaaatagga tactggaact
    41041 cgccctggcg ggtatggcgc gcctgaatta ccggcgtgta atagcctgta aactgcacgt
    41101 taccatagtt gtcggcacct tccatctgcc aggtatcaat gccgaactgg cgcatattgc
    41161 gggtatcacc gcctgcgcgc agccactctt gcaccgcgtt ataaacatta ctctggttgc
    41221 catacagacg cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt
    41281 taatcggcgc gccaacggca tctggctggt tcaccagaga gaaaggctgg gtaaatttcc
    41341 cgtctttata ttgctgtccg cgatcggttg gtttggaaga gcaggcggca agcattgcca
    41401 caaccgtgcc cataagaagg tactttaccc aacgtccttt cattgttctc ttcttcaggt
    41461 taaaaaataa ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg agcagcctct
    41521 ccctgacgca aattttgcac aaaaaatagg ctttagtggt ttgtttttgt tcaaaatcat
    41581 gctaaatccg tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc
    41641 gcatccacgg acgcggggtg gagcagcctg gtagctcgtc gggctcataa cccgaaggtc
    41701 gtcggttcaa atccggcccc cgcaaccaat tattgaacac cctaacgggt gtttttttgt
    41761 ttctgctctc ccataaaaaa agcgccattc agcgcctttt tatcatcccc ttctcgccag
    41821 cgtcgcccca tcggcaaaat acgctttaat tcccgcaaga atagactccg caacttcctg
    41881 ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc
    41941 gaccagaatg gagggaatat caggcgcttt cagcacggca aacccggcct gttcaacctg
    42001 atttttatgc agcttgttga ttttaccgag cttattcagc accgctttac caaacttaag
    42061 gctgtcggca atggtcagcg actgcaccat atcgaacatg gtgtggtcga catagcggtc
    42121 accgcttttg ctcacgccac caatcaagtc cgaggcgttc tgggtctgtg ccagatattt
    42181 tgccgcagta ctggttgcgc ctttggttga gagcgcaaac actgaggaac cgctcggctg
    42241 gcgactggta aaggcgtcgg catggataga gacaaacagg tcggcacgct gtttctgggc
    42301 ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt
    42361 catattgccc tctttctcga tcagagagcg cagacggcga gctatttgca ataccacgtc
    42421 tttttcgcgc gttttgtatt tccccaccgc accggagtct tcgccgccgt ggccagggtc
    42481 aagcataata acaataggac gatcgcgccc tgctttaccc ggttgtggac cactttgtgc
    42541 tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg
    42601 gtcctgcata tcctgtgcat tggccggata gaggtccatc accagacgct ctttaaaccc
    42661 ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat
    42721 ccgtacggtc tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc
    42781 acgaatttgt gccgccatcc ccttgagtac cgagttcagg tttacatctt cgatatccac
    42841 caccacgcgt tccgggttac tcaacgcgaa ctgcttatat ttcagctgac gatttgattc
    42901 taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctaac
    42961 cgcagccagg ctgacctgac ttacgctcaa tagccacata gcgcccgcgc cttgcagtaa
    43021 acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct
    43081 aagctgtgta acaagtaaac gactaatttg accggaaact ttagcgaaag acgcataatc
    43141 tgtcatctaa taaacggtaa acattctttt tatattcacg gcattactga taaaaaagtc
    43201 gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa tacaataatt
    43261 tcgaataata atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga gttggtcgag
    43321 ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg
    43381 ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga tatcgggttg
    43441 ttgcacagcc tcggcatccg tctggtggtg gtctatggcg cacgtccgca gatcgacgca
    43501 aatctggctg cgcatcacca cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc
    43561 aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat tactgctcgc
    43621 ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc atatcaacgt cgttagtggc
    43681 aattttatta ttgcccagcc gctgggcgtc gatgacggcg tggattactg ccatagcggg
    43741 cgtatccggc ggattgatga agacgcgatc catcgtcaac tggacagcgg tgcaatagtg
    43801 ctaatggggc cggtcgctgt ttcagtcact ggcgagagct ttaatctgac ctcggaagag
    43861 attgccactc aactagccat caaactgaaa gctgaaaaga tgattggttt ttgctcttcc
    43921 cagggcgtta ctaatgacga cggtgatatt gtctcagaac ttttccctaa cgaagcgcaa
    43981 gcgcgggtag aagcccagga agagaaaggc gattacaact ccggtacggt gcgctttttg
    44041 cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag
    44101 gaagatggcg cgctgttgca agagttgttc tcacgcgacg gtatcggtac gcagattgtg
    44161 atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg atattggcgg tattctggag
    44221 ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga gcagctggag
    44281 atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc ctgcgccgcg
    44341 ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt tcacccggat
    44401 taccgcagtt catcacgggg cgaggttctg ctggaacgca ttaccgctca ggcgaagcag
    44461 agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt
    44521 ggatttaccc cggtggatat tgatttactg cccgagagca aaaagcagtt gtacaactac
    44581 cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat tgtcggatgc
    44641 gacatgcgta acactcgtgc gtcgcatccg gcaattacgt ttattcccgc gaactaaaca
    44701 acgccgccag accactgcgc cgctcagtac gagtggcgat tgccgcactt aatatacgct
    44761 catcggcata cagcgacaga cggcgacgcg cgcgggtcac cgcggtgtat accagctctc
    44821 gcgttactac cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg aactccgatc
    44881 cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag cgactcggtt
    44941 gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat
    45001 ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta cgggcaatca
    45061 ttaccggtcg accttcgtac caacgagagt gcggatgacg atgaattttg cgcttctgtt
    45121 gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct tcccgcaggg
    45181 cgcacaaaag ttggtactca ttgaacgcct gaatgattaa atccggctcg gcacgcgctt
    45241 gcagcagatc cagataacgt ccataacccg caagagcttc ctcaagcatc gcaatataat
    45301 cttctccgct ctgtaaaagc cgtttttcga tatcagtaaa atcctgctga aaaacggttt
    45361 tcactgccgt tttatcacca cggttgatcg ccgcagctaa ctgaccaatg ccagaatcac
    45421 tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat gccgcttctg
    45481 tgccagttcc tgccggaacg tgagttcccg tcaggcggct tagctgcccg gcacgctcgg
    45541 cggtaaagcc ggcattggca taagcgcaga tatcgcccag cacagcccca gcctcaaccg
    45601 aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc aaggcgtcga
    45661 tcagtctcga catcataggc agatcgatca ttgacgcttc atctaccacc agcacatcaa
    45721 gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgc gcacccagca
    45781 atcggtgcaa agtactggca tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta
    45841 actgtcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta cccgttggtg
    45901 cagccagacg gatacggcag cgttcgccgt cggccatttg aattaacgct gccagtaact
    45961 tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag atccgccgcg
    46021 tcagcgccac tgccgctgca actttttgcc agttaatttc atcgcttact ggaaaaagtt
    46081 tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc acctcattga
    46141 agaagcgcgc taccgtccgc tcgttacacc acatgcgatt caaataaaga cgatcgccac
    46201 agaggatcat cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc agcaagcatt
    46261 cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc ggatgcgacg
    46321 cctcgttatt ttccagtcgt gaaagcggca aacagacgtg tccctctccg gcatcatgac
    46381 ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc tcccgccacg gtcagggcaa
    46441 attgcacatc cagcgggcgt agctgtttgt gctccacagc ttccagtaat tgcttttgca
    46501 atttcattac gcctcctcca gggtcatacc ggcaaacatc tcatccatca gggcaatcaa
    46561 cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat caacgccacg
    46621 caggaacaga tagataacgc cgccaaagtg acgatcatag tcgtaatcag caatgcgatg
    46681 gcgcagataa cgatgcagcg ccagggtata aagctgatat tgcagatcat agcggtgtgc
    46741 ctgcatcgct gctgccatag cctgttgggt gtaagccgaa ctgtcttcac ccagccagtt
    46801 ggatttatag tcgagcaggt aataacgccc ttcgtggcgg aataccaggt cgataaagcc
    46861 ttttaacatg ccgcgtacct gcatgaactc cagcggcggg cagccagcgg atagcgggtc
    46921 aaactggcga attagtgcat caagctggct ggcgatgagc ggttcgctaa tcggcaggta
    46981 aaactccatc tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa cacccgtttc
    47041 attgagaggt gcctggagga cagccgtgat ccactcggtc aacaccggtt cccactgcga
    47101 ttcaaagccg ccgagttcca gtttttcctg tacccagttc gggtcaaccg gctgggtaaa
    47161 atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc gcgggaactg
    47221 atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg cctgcggcat caacatccag
    47281 ccgtggcatc agatcctgag cgataccgtg accacgctgc tgcaagccgg agtagctggt
    47341 gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt ctgcagttaa
    47401 agcatcatta acctgccagg gctggttatc accagtttgt gccgtttgcc aggcaatatc
    47461 atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg gttccccttt
    47521 ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg tcaccttttt tatcgccacg
    47581 acggcgcacc agcggtgcaa cgccgagact gcaatgccaa accgaacgcg tcagcgccac
    47641 gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga cgctttctgg
    47701 cgcagcgtta agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa acgcctgatc
    47761 ctggacgcgg aaattggtga taaacggcag ccagaccaat ggatattcca gccctttcga
    47821 tttgtggatc gtgacaatct gcaccagatg tttatcactt tcgagacgca tttgttggct
    47881 ggaggcatta ctgtctggct cgaggatatg ttgcgataac cagcgtacca gcgcatgttc
    47941 actttccagc tgcgttccgg cttcttgtag cagttcgctg atatgcaaga tatcggtaag
    48001 acgccgctca ccgcctgccg ttgccagcaa gttttcagca atgttacgcg ccgacatcag
    48061 cgcccgcagc atcggcataa cgccacgttt gcgccagatt tgccgataac catcgaactc
    48121 ttcgactacc acatcccacg catgttcgtc attgttcagc gtttcgatat ccagcgcgtt
    48181 cagccccatc attgacgttg ccagcgcact acgcagggtg ttctcacgtt cgggcgtcat
    48241 caccgcctgc aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa cactgtcgcg
    48301 gttcgaaagg taaacggaag ggatttccag caacgttaag gcgtcgcgca cctgggcggc
    48361 ctcctggcgg ctgcgcacca gcacactgat gtccgaagca cgcaccggac gcgcgtcgtc
    48421 gccgttcatc agcaacgctt cgccccgctg tccggcttgt agccagtcgc ggatttgcgc
    48481 agcacatacc tgcgccatgg tactttgata gtcgccaacg ccgcagcttt cgccttccat
    48541 cagccacatt ttcatcgcag gctgcgtttc acctttaaac acaaaacgta acgcctgatt
    48601 tttcccggct gatttcactg gaataaacgg tatttcgcga aacatgaacg cgtcatcagt
    48661 ctggctgaaa agcttgttca cactgttcac cattcctggt gcggaacgcc agttggtgtc
    48721 taaagtgtag tgggcgtgaa cttcgctacg cgccttcata taagtgaaga tatccgcacc
    48781 ccggaatgca tatatggcct gtttcgggtc gccaatcagc aacaatgcgg tttccggctg
    48841 atggtgccag atacggcgaa aaattcggta ctgctggggg tcggtatcct gaaattcatc
    48901 aatcattgcc accgggaatc gcgtacggat cgccgctgcc aacacctcgc cgctttcgct
    48961 acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc cacggcggcg
    49021 tttttcacgc gctactgttt cgcggatctc agccaatgcg cgggttatca ccagatcacg
    49081 gatcgacaat ggttctgcaa gcagttgttc gatcgcctca aacagtggat gtcgcggggt
    49141 ttcccccccg gctttcgtgc gatcttctaa gaaacgctga gagaattttt ccagcgactc
    49201 cggtaactga taactgttgg tctcttcttc tgcccaggcg ctgatcttct cgatccattt
    49261 agcctgattg ctacggttaa acttgcgtcg atcaatacca gaagattcga tcagcgcatc
    49321 cagttcaccc actgcgtcgc gccactgctg ttttaccgta tcaatacgcg ccacaatttg
    49381 cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt gctttgataa ccggtgcttc
    49441 gccttgcaga taacgattaa tatcgcgcag caacgcctgc ggccctttcc aggtttcaaa
    49501 gaccaccagg gcaatttcac gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca
    49561 ggcctggtag cgtagcagag actcatcttc aatcagctgc tgctcaaaca gcataccgga
    49621 ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa agactgccgc
    49681 ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg gcttgcgctt tatcgtcgat
    49741 ctcttccagc aggcgttcgt acagtggatt gtcggtggtt tcacgcagac aggcgatgcg
    49801 caactcgtgg atattgctac ggatacgacc gcgcaattct gccgtggcag cctcggtaaa
    49861 ggtgaccacc agcagttctt caacggtcag cgggcgggga aaggcggcgg aaccgcctag
    49921 tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg tgccggcaga
    49981 ggcttcaatc aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac
    50041 atcactcatt cttttcactc atcaggggca ttgtttgctg caacgcgctg acgttctccc
    50101 acactttcca gccttcagga tgcacatatt cggctttccc gttctggctg ccggaaatct
    50161 gcgacaggat agccatgcct tgcggctcga ccaccgcctg atggaagaaa tcagcaagtt
    50221 tttgcggcgt cagcagtttt atctgggcca cgattttatc acgcgaatcg aagcgcatat
    50281 tgccgcgatc gaaatcttta cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct
    50341 gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc ttcatcgctc
    50401 gcaattttgc ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag aatgaaggct
    50461 gtttatcatt gctttgcaaa aggaagccca tgccccactg acgccccacg ctcattggaa
    50521 acgcaaacac ggcatagccc aattgttctt cggtacgcaa ctgattgtag aaccacggct
    50581 gtacgatctg ccccaacaga gagctatagg ctgagctggt gtattcatcg tagccagtcg
    50641 gtacaaatac cgctgccagt gcggagtcgg tgctgttacc ggctttttca aagatgacgg
    50701 attgtttttt atcgaccact acatctttgt ttcgacacca ctctgaacca tcagcgccca
    50761 actgtttttg cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg ttgccgataa
    50821 ccataaactc tggtcgagcc cctgatttta aggcgtcgcg ataggccagc acctctttca
    50881 acgtaatgga gggcaaaatt ttacgccgtt catctcgcga gaagtacggc acttgcgaga
    50941 gcatctgcgc gggcataatc gcctgctcaa acgctttacc cttttctgcg gaatccatca
    51001 tctggttata ccaggacttc gcctgctcaa gctgatcttc cgtagcggta tagctaaagt
    51061 acccctcgag caatgcctgg aacagctgcg gcagacgctg ggtgtaacca ttagcattaa
    51121 ccataaggcc gttgttagcg ttggtggaaa aacttatgcc accaaccgac gcctggttgc
    51181 ttaactgatc aagcgccagc cctgcgagat aatcattgag cgcaaacatc acctgattgc
    51241 gggcgctgtc catggctttc ggattacgca aaatcaggct gacatcagct ttgggttcgc
    51301 tggaaaaata acggcttggc gcatacacca cgcgcagatt cgactcatca acaatcagct
    51361 ctggatggtc atatttcttc tctgacttaa tcagcgagaa atcgtcagga atataggggt
    51421 taagctctgg caaagagagc gcaatgtcgg cggctttttt ctgccagtcg gcgaaagttt
    51481 gtgcgctgat tttatcgacc tgatacggcg catcgacaaa gtaagccgtt ttgttgtgcg
    51541 gctctttcgg gctgatatac cagatacgcg cattctgcgg cgtcatcatc gccagacgtt
    51601 cctttactgc tttagcatcg taccgatcgg caatattgac tgcatccagc gtatgctcaa
    51661 caggaacgcg aatcatggta tctgccagcc attcgacgta atccatatca cgggtgattg
    51721 acggataacg gaagtcgata tccagcacat tcgccagttc atcgaagtat tgtttatcga
    51781 tccctttttc acgtaacaaa ttgaggtagc taaaaatagc cgccacaacc tgatcgcgat
    51841 tcgccagacc tttatcggtt aaagacgcag agatcgctaa tacgccgctg ttgccgttga
    51901 cgataggatc ggagttggcg ctaatgccct caactaatcc ctgcttttgc agccagtcgg
    51961 aaagtgtacc tgggctgcga ttaccaatca gataggtaat caattcatcg gttttgctac
    52021 ggaatttcgc cgagttgtta tcaatacgaa actctacgcg caacacttta cgcggcagcg
    52081 ccgggacgta atgaatgata atgccctttt gcgcgtcggt gactaccggc acggtgattt
    52141 ccggtttttt gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca
    52201 actccggcag cggtttattg ctgtaaataa ccgccttcat cagattggcg gaatagtact
    52261 tctcgtggaa atctttcagc gcctgctgca ccggattacc aggtttgtcg cttaaagttt
    52321 cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt tctgcgctga
    52381 cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt taattcagcg ttcactgcat
    52441 tacgctcacg ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca tcagccaggc
    52501 ggtctaccgc accaggcaag gcgtcgttct caacttccag atagaaagcc gtgcgatacg
    52561 gcgcagtgct ggcattgtga ctaccgccgt gcattttgag atattcggcc agactgtcag
    52621 cctgcgggta ctttttcgac cccatcagac tcatatgttc aaggtaatgt gccagcccct
    52681 ggtacgcctc gggatcttcc agcgacccaa cgggcaccac cagcgccgag agcgatttaa
    52741 ctgcctgcgg atcagaaacc agcaagacca ccataccgtt atccagacgt atagcctgat
    52801 actggcggtt atctttatca cttttacgga tggtttcctg aatcggctgc catcccgttt
    52861 ctgcctgact taagggtgcc caaagggcaa ctaacaacaa taatgctttg aaccaggtgc
    52921 tgcggggcat tcacggacct cataatcaac ttaattttct gtccagattc aacacgttaa
    52981 cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag tcagcctatg tttatataac
    53041 catcagtccg tgactggtgc gcatcataaa gtaagcggat aaattgcgca atttttatac
    53101 agcactcatg actgattaaa gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg
    53161 atggcctcca ttgtctctgg tgttaattgc cgccagagcc gttgatacca gatatcatca
    53221 ccttcgccac gcaccatcat gttgccttcg taagcctgca ggaatttcgt acgggctttt
    53281 tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg cgtcataaca ggtttttagc
    53341 cacgcgccgc cactttcagg taacaccagc aatggcgcgg acatcccttc acgatacccc
    53401 tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg caagcggcgg aaaacgccac
    53461 tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac caccgctggc acagtagaca
    53521 aggtgttcca gccaaagttg cattccctgc gccacactta ataaagaggg acgccagcgc
    53581 aacaagccat ccggctgcac ctgcggcaac cagccagtta tctgcacacc gttgcaggcg
    53641 agatcaattt ccatactctg ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc
    53701 tgcatctcct ggcactgtgt ttcccagaaa atttcaccaa aagcgccata cggtaaatcc
    53761 cctgccgccc ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac cagtgcattc
    53821 aataactgct gattgatttg ataacgacta agcccttcaa gaataaatgg ctcggtgtcg
    53881 gggatttcgc tgtcttcagt acggaagttc acctgcaaac gcatctggaa gaacgcccgc
    53941 accggatgtg cccagaatcg ttgtagcgtt tccagcggca cggtttccgg taaggtaaac
    54001 ggcagcggct gaacaaattc agaatgtgct ttaccagcct ggctggccgc aggtagccat
    54061 tcacgagcat agctttgtcg ttcgcctggc tggtagtttt gtggatcaaa cggcatccgg
    54121 gtatggaggc aagtaagatg cgcttttacc cttgcctcgc tttcatcaca gttgagcgct
    54181 tcatcgcccg gtagataatg actttgcccg atgtagtcga tcagttcctg caccagtacc
    54241 gacgggaaac gttcactgtt atcctgaatg gagcgcccga tatagctgat ataaagtttt
    54301 tgttgcgcgg aaattaacgc ttccaggaac agatagcggt catcatcgcg acggctacgg
    54361 tcgccacgct tcggtttctg gctcatcagg tcaaagccca atggcgcaag ctgacgtgga
    54421 taaacgccgt cgttcattcc cagcaggcaa accactttga acggaattga acgcattggc
    54481 atcagagtac aaatgttaac cggtccggcg agaaaacgct ggctgatacg ttcttgatcc
    54541 aggcgctgtg ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc atactgcgca
    54601 cctaaacctt cggcgataat cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg
    54661 gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg caaccactct
    54721 tccagcggac gctcctgtgc cagcccgcga cgccagatgt ttagctgcat tagcagggaa
    54781 gccagatgcc ccaccagttc tgcaattaag ccgctcgatt catcataagg tagaaccgat
    54841 tgccactcgc cctgcgcgct ctccatcgcg taacccagca acatgcgcgt caggccaaac
    54901 cgccaggtgt gttgaccggt agcggggagt tccagctcgc gaacgttgtc gtcatctatc
    54961 ccccaacgaa tgccggattc gttgacccac tggcgtaaat aacgcagccc ttcttcggtg
    55021 atgtcaaacc gcgccgccag caccggcaca tccagcagcg ccagcacatc ctctgacaca
    55081 aagcgactgt caggcagtga taacaggctg ataaacgctt ccagtaccgg atgtgactgc
    55141 cgcgcacgac ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac
    55201 actgcctgaa taaacggact gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc
    55261 ggagtaagtg tcgggtcttc ctccagcatc gccagcaggc gatcgtgtaa aacttcaact
    55321 tcacgctgcg ggctatggca aacgtggaag gtgatactgc tatccagtgg atcaagcggg
    55381 cgtttgttat cgctacggga aaactcttcg atgttcacac cagcaacggc gcggttttcc
    55441 agttccagaa tgtcagactg aatgttatgc agcaggttat ctggcatcac atcgacaaag
    55501 gcgtccagct cctgactgct atccagatcg gaaaggagat agatatagtc gcgccccaac
    55561 ttgccccagg aggccagtag cgggttgccg acatcctgtt caccgtcgct gttaaagagc
    55621 tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc gatcttcaaa actgtgtcga
    55681 cgctgacgag tcagcagttt cgccagataa gcaggatctt taatatcgcc ccagtaataa
    55741 cggcaggggt tggtaaacag gagatggatt tcaatatgtt tacccagcgc ctggagcgcc
    55801 tggagataaa caggcggtaa cgcggaaata ccgcatataa agacgcgcga aggtaacccc
    55861 ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag attggcgcgg
    55921 tgccagcgcg gttgcccgag ttgatgggta tattccacca acgccttcca caacggcgct
    55981 tgccaggcct gtgcttctcc cagcccttca accaaatgtc ctgtttccca ctgtgccagc
    56041 cagtccggac gatagaccag atactggtca aacaggtccg ccgcttttga agaaagctgg
    56101 aacagttttc gcttgtcgct atcgtcagtc agataatgcc gcaacagggt aaagtcttcg
    56161 cgatccagca attgcggcag cagagtcatc agtttccagc tcatgctctg tttgttaaag
    56221 gcgctctctt tggggatttc cggtaacacc cggacgaaca tatcccagat aaagctcgca
    56281 ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt gcgacagggt catttgcagc
    56341 cactgcgcca taccggtact ttgcaccaga atcatctctg gttcgaaagg atcgtccagc
    56401 cgttcgcgtt cgacaataaa ctccatcaac gcttccagca cgtccagacg attggaatgg
    56461 tagaccctta acatagcggc tcctgactac tgacgattcg ggcaatgcag gcgggtcatc
    56521 tcgccttctc tgcccccggg tgaaactagc gtaacgctga tgctgacaca tcccgcctgc
    56581 gatgtctgca ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt
    56641 tgctgccagc catgccgcca gagctgttgg tactggtttc tgctggcaag actgttcatt
    56701 aatgttcgct ggaaacccga taacgcagtg acaatcatca ccatcaacac catcgccaac
    56761 attacctccg gcaggctaaa gccttgttga ttcttcaggg aagctgacat aacgccctct
    56821 ctttcagcgg acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt ccatcaatga
    56881 cttcgcctgt tcgccacaac gaaacgcctt cataatcggc aatcaataag gcttcattat
    56941 ctgccagtaa acgcaaacaa acctgggcat cggttccagc gtactgcgag cactgaactg
    57001 ctggctgcgt ctgccaggag tgcatttttc cccacgccag cgccgactga acgatggcct
    57061 ggcggcgcaa tgactgactt tccatgctca cgcgagaggc aaaactgcga tcctgttggc
    57121 tcattccttg taatagcaag ctacctaaaa ccagcagcat caggaccaga gccagtgacg
    57181 aaacaccctt ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg cattcaccac
    57241 ggtttgcggt tcagacttac tggcagcacg catattaacc gtcaacaccg gcgagaagcc
    57301 gctgacatcc tgatgtacga cctgaaaagt gtcgataatg atggcatccg gattagtgac
    57361 tttatcccag cccttacctt cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc
    57421 cttcagacga aatccaatct ggtcagactc ttttaccggt tcgcgatccc agataccgtt
    57481 actgttcgca tcccactgca caatgataca gtcaccctgt ccgacaattt ccagcccttc
    57541 gccggtacag ctgccatgac aataacccgc cctctggaga tgcttcgcga cggtaaatac
    57601 ccgcagccag atttcatctt ccagcgccag cttacgggtg ctcgttaaac tttcacgctg
    57661 taacgcaggc agaaagcgtg cagcccccag caacaatacg ctactgatcg ccatagcaat
    57721 caacacttcc agcagagaaa aaccttgctc ttttacaggc atccttctgt ttctccttgc
    57781 tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc accactcgcc cgttgagttt
    57841 ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt
    57901 gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa agaccaatgg tgaactgcca
    57961 tgacaggtat tggccccagc agcggaactc acaaggcacc ataacgtccc ctccctgata
    58021 acgctgatac tgtggtcgcg gttatgccag ttggcatctt cacgtaaata gagcaaatag
    58081 tcccgcgcct ggctggcggt ttgccatagc cgttgcgact gctgccagta ttgccagcca
    58141 tagagtccac ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc
    58201 gtataaccac gttgtgtttt catgccggca gtatggagcg aggagaaaaa aagacgaggg
    58261 ccagtttcta tttcttcggc gcatcttccg gactatttac gccgttgcag gacgttgcaa
    58321 aatttcggga aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac gcacactggc
    58381 gtcggctctg gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgt
    58441 ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta
    58501 cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga
    58561 tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc gcccacttcc
    58621 agatcacact gctgcgccat catatgcacc agtaacgcgt agctggcaat gttaaacggc
    58681 aggccgagga aaacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc
    58741 acatagaact ggaagaacgc atggcacggt gccagcgcca ttttatccag ttcgcctacg
    58801 ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc
    58861 gtagtgatct ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca ctgtttacca
    58921 tacactggtc cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt
    58981 tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc gtggatgatg
    59041 gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc
    59101 atctgatgac caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct
    59161 tcgtcgagca ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt
    59221 tgctgtgggc tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg
    59281 atggaaagaa tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg
    59341 ggctggcgga aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcca
    59401 gagacagctc ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcacc
    59461 acaccttcca gcagcagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg
    59521 tcgaaaatgg attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag
    59581 cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta
    59641 ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca
    59701 gaaacctgga agaaggaacg tttagtacgg cgggcaaaga taatcatcac cacgataacg
    59761 ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga cacggaacag atacagcgga
    59821 tcggccataa actgcgggaa attgtagaac agaacataac caatacgtcc cccgaggaag
    59881 acgccgagga agcccgcata gagtaagttt tcaacttcat ttttggtcca gccgctgccc
    59941 ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc
    60001 aggccgtacc agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga
    60061 aaatgcagat agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga
    60121 acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca
    60181 aggggacctg gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac
    60241 cctccgcgaa tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatggcga
    60301 acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcataa
    60361 tcaatgcgcc gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg
    60421 cgatacccaa gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac
    60481 aaacggagat cgattccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac
    60541 attgcaggat gaagactgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc
    60601 agaatgtatt gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga
    60661 tgcggcagca taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga
    60721 atttcgtaac cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct
    60781 tcatcaactt catcgaggct tgtgaccatc ggcaacagaa tattcaggtt gcccgtagcg
    60841 gcattagcac gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta
    60901 atgcgaatcc cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc
    60961 tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc
    61021 agcatcccct gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg
    61081 aatgggattt cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca
    61141 tgttccgggc ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc
    61201 gcgggtaaat tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc
    61261 cgctgatatt cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca
    61321 tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta
    61381 gggatcccca gcgcacgtac catgattgca gcatgggagt tggctgcgcc atcgcgcacg
    61441 acaacaccca ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct
    61501 gccaccagaa tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga
    61561 aacagcaatc gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg
    61621 cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc
    61681 accgagcctt tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg
    61741 tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg
    61801 ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc
    61861 agagccggat ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc
    61921 tgccagcctt cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg
    61981 cgatattgcc caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg
    62041 gcaagcgtca ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt
    62101 accaccagta caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg
    62161 aaacgttctt cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca
    62221 aggtttatcg gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc
    62281 gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt
    62341 cgatcatgat cggcaaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg
    62401 gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt
    62461 tcgcgcaggc gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat
    62521 gcgttttgtg gttttggcgt attttcctgc agtgacatca ccacgctcgc gaactctttc
    62581 attaccctac ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa
    62641 cttacccatc gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca
    62701 tcgccgctca ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc
    62761 gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca
    62821 aggattcgaa cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc
    62881 tgctctgcgg attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca
    62941 aatcgccggg cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg
    63001 cggtagccat cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat
    63061 tgtttcatac tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca
    63121 attgaataac tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt
    63181 ttattcacct tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc
    63241 ggagcattct gaataagccc gatatagaca ttttaaaata ttcattatta gtcaatgcat
    63301 tacatgtaat ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta
    63361 cgggttggcg aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag
    63421 cactgaacca ctaaaaactg gagtttcatc gcacgtcaag gctgtaaatg gaaacagtag
    63481 tggaggtttt tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta
    63541 ttttcagtga ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc
    63601 ctaaaaagca atataaatca gggaattgaa caacacatgt ggaaaaagtt acactgcgaa
    63661 tattcggcac ataattgctg tttgtttttt aatcaaggta tcatgacatg tcccaacctc
    63721 gcccactgct ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt
    63781 ctggttatac attgggagaa ctggcggcac ttgccgggct tgttacgcca gagaatttaa
    63841 aacgcgataa gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga
    63901 gtaaacctga gcaagatttt gccgctctgg gcgtggaact taaaactatc cctgtggata
    63961 gtcttggtcg tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg
    64021 gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg
    64081 ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga
    64141 gcccgaatga agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga
    64201 ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac
    64261 cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc
    64321 tgacgctgcc acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc
    64381 attttctgat ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct
    64441 tcattttttt ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg
    64501 gcttgcgctc ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt
    64561 cctttctctg gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg
    64621 gttggcggga gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg
    64681 cctgacaaat ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct
    64741 gcttctgggt ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga
    64801 aggtgaagaa gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat
    64861 tatgctgctg gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga
    64921 tcacctgttt attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc
    64981 gcgctcgatt ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt
    65041 cctgattctg gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg
    65101 ttacatctac ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa
    65161 caaaaagaat ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt
    65221 ttaccctcac agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag
    65281 actattatca taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt
    65341 tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc
    65401 aaagatggcc gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg
    65461 gtgagttatc acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa
    65521 attattgaac gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc
    65581 cttttccctt ccctctgcca tttttatatt cctcatgtcg tgattataaa aaggaaacgg
    65641 ctatgcaata tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca
    65701 cgatgacgtt tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg
    65761 tcgctcaggg cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg
    65821 aaacgcaagg gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg
    65881 aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc
    65941 gcccggatca ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc
    66001 gcctacagac tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact
    66061 gcttcggcaa actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata
    66121 cgctggacgc actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga
    66181 acgaaactgc atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc
    66241 gtattgtcac cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg
    66301 cagaagtcag ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca
    66361 cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta
    66421 gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata
    66481 tcgccagacg tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac
    66541 cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg
    66601 aaagtttgca tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg
    66661 tttatactta tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg
    66721 acggcgctgc cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat
    66781 gccaatgggc acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa
    66841 caacatggcg ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct
    66901 ttttttaccc cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac
    66961 gccaatcagc atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag
    67021 taccaccacg ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac
    67081 taacttcgct gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata
    67141 ggtgggcgta gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa
    67201 acgcagcgtg acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc
    67261 accattgcgc catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt
    67321 ccaggactgc cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc
    67381 accaccgtag gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac
    67441 accaccggct acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt
    67501 agctttcact aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga
    67561 ataggctgca gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca
    67621 gatactggct gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc
    67681 gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac
    67741 catttgcagg atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt
    67801 ggcaaacagt agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt
    67861 catccccttc gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg
    67921 cttcgtctac ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca
    67981 tctgtttcag atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg
    68041 catactgttg caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct
    68101 cgcctttgct ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa
    68161 gtgccagttg ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt
    68221 ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt
    68281 cataccagcc gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac
    68341 ctggcttctc cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc
    68401 cgccctcttc gataccaggg accgacaaca ggcgcgcatc cattcctggt agaatacgcc
    68461 ctaccgtacc gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg
    68521 tcacgccgta gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac
    68581 tttcttgtaa tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt
    68641 tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtgcc gaacaacacg gtgcaactgc
    68701 ggtcatacac caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt
    68761 ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg
    68821 gtaacgccga cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga
    68881 cattcgccag aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg
    68941 aggtaaagag gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca
    69001 tcagcaaatg agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt
    69061 ccagatagac ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
    69121 cgagaaactg gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca
    69181 gcccttttac cccggcagtg tagttcatca ttgcgggcat gcggcgacgg gcgatggccc
    69241 caaaaatcac tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
    69301 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
    69361 aggagtctgg ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca
    69421 ttgcactcag taaagattcg tacagcgttt cacgcgggcg caccgccatt cgcgcttcca
    69481 tcattatttg atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat
    69541 ccggcatcgc cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac
    69601 gtttaaccag acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag
    69661 gaataaccgt tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca
    69721 gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca
    69781 ccagacgtac cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga
    69841 taaatgattt cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca
    69901 ctggacgcac aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag
    69961 gcgtaattag aacgcgctcg cccttcagtg cctgggggtc acccgtaacg cgaacgcgat
    70021 acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga
    70081 ttcagtaaaa gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt
    70141 aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag
    70201 cgtaccgaat aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga
    70261 ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg
    70321 tcggttactg ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg
    70381 tgtaattcat gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac
    70441 tttagcaacc tgaagccaaa cgccaccagc gatcaatcgc aacagcgttt cgcaaagtgt
    70501 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca
    70561 cgatgaaaac acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta
    70621 agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
    70681 cgtttcagtc gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc
    70741 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
    70801 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
    70861 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
    70921 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
    70981 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc
    71041 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
    71101 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
    71161 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
    71221 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg
    71281 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
    71341 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc
    71401 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
    71461 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
    71521 cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct
    71581 cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
    71641 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
    71701 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
    71761 gaacttctgg taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc
    71821 ctgtatcatg cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcaa
    71881 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataatggc ccggcgacga
    71941 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
    72001 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccgaca tcaaccagca
    72061 caaagtggcg gctacccatt tgtttgacgc tccgcacctg agtaattaac acgccagact
    72121 gcgctaccag aaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag
    72181 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
    72241 caccctgttg ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
    72301 cgaattcgat gacctgacgt accatagcac cacacacctg ttccagatgg gcataatcaa
    72361 cgccagaacc aatgtgcatg tgaatgccga ccagctgcaa atgatgacgt tgtatcacgt
    72421 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
    72481 tggttttttg gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc
    72541 ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt
    72601 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
    72661 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
    72721 agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac
    72781 aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa
    72841 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
    72901 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
    72961 aagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
    73021 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    73081 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag
    73141 cacacctgct acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg
    73201 tgatcgggct aaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac
    73261 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg
    73321 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    73381 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    73441 taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    73501 atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct
    73561 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    73621 cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc
    73681 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    73741 acagcgccgc gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc
    73801 cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg
    73861 ttccgttcac cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggtgcagg
    73921 cgtttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt
    73981 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcagtatc
    74041 aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa
    74101 aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    74161 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    74221 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    74281 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    74341 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    74401 ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc
    74461 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    74521 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga
    74581 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt
    74641 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    74701 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccgat
    74761 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg
    74821 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    74881 ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag
    74941 agccagaacg taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata
    75001 atcatattcg acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc
    75061 agcggctgaa tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg
    75121 gcaatacaca tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg
    75181 ttatcaaact gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa
    75241 ccaattttca gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc
    75301 gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta
    75361 aagcccgcca ttttgaagat acgcggcgca tagtacatga tgatgttcat accggtaaac
    75421 tgctgcatcg cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc
    75481 ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc gttgagttct
    75541 tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga
    75601 cgcccctttt ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc
    75661 aggactgctg gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac
    75721 gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg
    75781 atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa
    75841 gacgcgatcc cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg
    75901 gtcgcaaaag cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg
    75961 tatttacgac ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcaccg
    76021 agcatcatgc tactgaccac ccattcctgc aaacggctgg tcagcacaaa gtgatcggta
    76081 atgaacggca acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc
    76141 gcagcagcta ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt
    76201 aaagcagatt ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt
    76261 gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga aacagcataa atatggatta
    76321 aattgctgcg gcatgtcgtt atgtgatgga tattccaatt ttcaaattaa gttgaattat
    76381 tgagattatt attaaccacc taattttaca gcagataaaa ttcataaagt tcattaattg
    76441 ataattgata tggattattt cataaccatg atatggatta tgatgatcta caggtataaa
    76501 aaaccctgcc atgcggcagg gtcataaaag taagaagaat gaattaacgc gccagccaac
    76561 cgccatccac ggcaatggta taaccattca cataatctga agcgctggag gcaaggaaca
    76621 ctatcggccc catcaggtca ctcggcagtc cccaacgacc agctggaatg cggtcgagaa
    76681 tttccgcgct acgttgttca tctgcccgta gttgttgagt attgttggtc gccatgtaac
    76741 ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc atcaatcgcg
    76801 tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga
    76861 aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt
    76921 tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc
    76981 agtccttttc gctgaactcg agagcatctt cgcggcgaat caatccggcg ttattcacca
    77041 ggatatcaat atgaccaaac tccgctaccg cgcgatccag cagtgctgga ataccatcaa
    77101 tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgcttga
    77161 tggtttcagt aggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc
    77221 ccaacgccat cccctggccc agcccagtat cacagccagt gacgaccgca actttacctt
    77281 cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccc acaactagcg
    77341 caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac cgaccatgcc
    77401 ccagataaag gtataagctt tggttccgac gccggaatga atcgaccagc tcggggagat
    77461 caccgcctgc tcgttatgca tcacaatatg acgcgtttct tgcggctgcc ccatcatgtg
    77521 gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg
    77581 agtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact
    77641 caattggcag gtttccagca catccgggac aaaatatttg ttaattgtgc gacggttact
    77701 ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt ttttggtggg
    77761 atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt gccggagtgg cggtatcaat
    77821 actggcaaag acaacttctt ttgcaccttt acccacatac agggcgtcgc ggtgaccgat
    77881 ttcatagcat tggccatcga cagtaatcgt accggcaccg ccaatattga taacacctaa
    77941 ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc cgccaacgga
    78001 aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt ggctgtaaac
    78061 catggtgtac tcatcggcga caaatacctc ttcaaccaaa aattcattgc gcagcccctg
    78121 ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca catcaacctc
    78181 cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc gttttctcta
    78241 taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca atgtttcatt
    78301 tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga atatgtcatg agaatggaaa
    78361 aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca acagattaat
    78421 gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt tcaatggtca
    78481 atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg gcattacgtt
    78541 tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct atcgggtgac
    78601 cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca agcatcttgc
    78661 caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg acatcagcca
    78721 actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca attcccatca
    78781 atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg ggtaaattca
    78841 acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg
    78901 atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa cgggctaaac
    78961 cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gtctgaccgt
    79021 tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca atcgccgctc
    79081 gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt
    79141 cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaacc
    79201 catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg gtgcggctat
    79261 cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatcag
    79321 cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagccca gaaccgcaaa
    79381 cgtcattaat agtgattgca gaaacgctat taggaagacc acctttaata gccgattgcc
    79441 ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg
    79501 catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta ccaagttcca
    79561 cggcggaatg acccgctaac gcaccgcgaa agcagccgat aggtgtccgt aacgccccga
    79621 caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg
    79681 ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag gaaatatgcc
    79741 tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac ccaccaatag
    79801 aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcca
    79861 ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc taaagaagaa
    79921 actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct
    79981 attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct
    80041 ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcgaa aacatcagca
    80101 ggtgaaggga taacgggcgc agtaacacac tactatggca agaagattgg taatctgatt
    80161 accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc
    80221 aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg
    80281 gtgagtctgg gtgttgtatt aatcctgaat ctcatttttc tgatgggacg ccatgcaacc
    80341 attcgggtaa tgggtttttt ggtattccca ttgattgcct atttcttatt tctttccatt
    80401 taccttgtcg gtagttggca acctgatcta ttaacgaccc aggtagagtt caatcagaat
    80461 acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag ccatacgccc
    80521 attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc tatggataaa
    80581 tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact gttctttgtc
    80641 tttagctgcc tgctttctat tccaccttcg tatattgaag cggctaaaga agaaggggtc
    80701 accattttat cggcgctttc tatgctgccg aacgccccag catggctgtc aatttccggg
    80761 attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt
    80821 gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg ttggtgtaaa gaaaagtcgt
    80881 gcatttaacc gcgcactatc aattatgttg gtatcgctga tcaccttcat tgtttgttgc
    80941 attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata
    81001 cttttcatca tgcctacgtt gtcaacgtat ctgatcccgg cgcttaaacc ctggcgttcc
    81061 atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc
    81121 tgatatccat tgtgaatggt tcaaaattgc tacaattttg aaccattcca gttttcataa
    81181 tgttcattcg ttattattcc gcactatcac ttccctcatc atatc
//
LOCUS       DAZFVO020000002        67832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFVO020000002 DAZFVO020000000
VERSION     DAZFVO020000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 67832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.002"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            197..1131
                     /locus_tag="ACHKNB_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /pseudo
     CDS             197..1131
                     /locus_tag="ACHKNB_RS00345"
                     /old_locus_tag="pgaptmp_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1187..1729)
                     /locus_tag="ACHKNB_RS00350"
                     /old_locus_tag="pgaptmp_000070"
     CDS             complement(1187..1729)
                     /locus_tag="ACHKNB_RS00350"
                     /old_locus_tag="pgaptmp_000070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4193659.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRLSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1713..2024)
                     /locus_tag="ACHKNB_RS00355"
                     /old_locus_tag="pgaptmp_000071"
     CDS             complement(1713..2024)
                     /locus_tag="ACHKNB_RS00355"
                     /old_locus_tag="pgaptmp_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HGU4193660.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2209..3099)
                     /gene="cyoE"
                     /locus_tag="ACHKNB_RS00360"
                     /old_locus_tag="pgaptmp_000072"
     CDS             complement(2209..3099)
                     /gene="cyoE"
                     /locus_tag="ACHKNB_RS00360"
                     /old_locus_tag="pgaptmp_000072"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HGU4193661.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3111..3440)
                     /gene="cyoD"
                     /locus_tag="ACHKNB_RS00365"
                     /old_locus_tag="pgaptmp_000073"
     CDS             complement(3111..3440)
                     /gene="cyoD"
                     /locus_tag="ACHKNB_RS00365"
                     /old_locus_tag="pgaptmp_000073"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HGU4193662.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3440..4054)
                     /gene="cyoC"
                     /locus_tag="ACHKNB_RS00370"
                     /old_locus_tag="pgaptmp_000074"
     CDS             complement(3440..4054)
                     /gene="cyoC"
                     /locus_tag="ACHKNB_RS00370"
                     /old_locus_tag="pgaptmp_000074"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HGU4193663.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4044..6035)
                     /gene="cyoB"
                     /locus_tag="ACHKNB_RS00375"
                     /old_locus_tag="pgaptmp_000075"
     CDS             complement(4044..6035)
                     /gene="cyoB"
                     /locus_tag="ACHKNB_RS00375"
                     /old_locus_tag="pgaptmp_000075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HGU4193664.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6057..7004)
                     /gene="cyoA"
                     /locus_tag="ACHKNB_RS00380"
                     /old_locus_tag="pgaptmp_000076"
     CDS             complement(6057..7004)
                     /gene="cyoA"
                     /locus_tag="ACHKNB_RS00380"
                     /old_locus_tag="pgaptmp_000076"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HGU4193665.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7466..8941)
                     /gene="ampG"
                     /locus_tag="ACHKNB_RS00385"
                     /old_locus_tag="pgaptmp_000077"
     CDS             complement(7466..8941)
                     /gene="ampG"
                     /locus_tag="ACHKNB_RS00385"
                     /old_locus_tag="pgaptmp_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HGU4193666.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8985..9563)
                     /gene="yajG"
                     /locus_tag="ACHKNB_RS00390"
                     /old_locus_tag="pgaptmp_000078"
     CDS             complement(8985..9563)
                     /gene="yajG"
                     /locus_tag="ACHKNB_RS00390"
                     /old_locus_tag="pgaptmp_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4193667.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9868..10185
                     /gene="bolA"
                     /locus_tag="ACHKNB_RS00395"
                     /old_locus_tag="pgaptmp_000079"
     CDS             9868..10185
                     /gene="bolA"
                     /locus_tag="ACHKNB_RS00395"
                     /old_locus_tag="pgaptmp_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HGU4193668.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10529..11827
                     /gene="tig"
                     /locus_tag="ACHKNB_RS00400"
                     /old_locus_tag="pgaptmp_000080"
     CDS             10529..11827
                     /gene="tig"
                     /locus_tag="ACHKNB_RS00400"
                     /old_locus_tag="pgaptmp_000080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGU4193669.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            12073..12696
                     /gene="clpP"
                     /locus_tag="ACHKNB_RS00405"
                     /old_locus_tag="pgaptmp_000081"
     CDS             12073..12696
                     /gene="clpP"
                     /locus_tag="ACHKNB_RS00405"
                     /old_locus_tag="pgaptmp_000081"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HGU4193670.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12822..14096
                     /gene="clpX"
                     /locus_tag="ACHKNB_RS00410"
                     /old_locus_tag="pgaptmp_000082"
     CDS             12822..14096
                     /gene="clpX"
                     /locus_tag="ACHKNB_RS00410"
                     /old_locus_tag="pgaptmp_000082"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HGU4193671.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14284..16638
                     /gene="lon"
                     /locus_tag="ACHKNB_RS00415"
                     /old_locus_tag="pgaptmp_000083"
     CDS             14284..16638
                     /gene="lon"
                     /locus_tag="ACHKNB_RS00415"
                     /old_locus_tag="pgaptmp_000083"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HGU4193672.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16829..17119
                     /gene="hupB"
                     /locus_tag="ACHKNB_RS00420"
                     /old_locus_tag="pgaptmp_000084"
     CDS             16829..17119
                     /gene="hupB"
                     /locus_tag="ACHKNB_RS00420"
                     /old_locus_tag="pgaptmp_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HGU4193673.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17311..19182
                     /gene="ppiD"
                     /locus_tag="ACHKNB_RS00425"
                     /old_locus_tag="pgaptmp_000085"
     CDS             17311..19182
                     /gene="ppiD"
                     /locus_tag="ACHKNB_RS00425"
                     /old_locus_tag="pgaptmp_000085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4193674.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAQQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19333..19704
                     /gene="ybaV"
                     /locus_tag="ACHKNB_RS00430"
                     /old_locus_tag="pgaptmp_000086"
     CDS             19333..19704
                     /gene="ybaV"
                     /locus_tag="ACHKNB_RS00430"
                     /old_locus_tag="pgaptmp_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGU4193675.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19798..20196
                     /gene="fadM"
                     /locus_tag="ACHKNB_RS00435"
                     /old_locus_tag="pgaptmp_000087"
     CDS             19798..20196
                     /gene="fadM"
                     /locus_tag="ACHKNB_RS00435"
                     /old_locus_tag="pgaptmp_000087"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HGU4193676.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20248..20943)
                     /gene="queC"
                     /locus_tag="ACHKNB_RS00440"
                     /old_locus_tag="pgaptmp_000088"
     CDS             complement(20248..20943)
                     /gene="queC"
                     /locus_tag="ACHKNB_RS00440"
                     /old_locus_tag="pgaptmp_000088"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HGU4193677.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(21008..22708)
                     /gene="ybaE"
                     /locus_tag="ACHKNB_RS00445"
                     /old_locus_tag="pgaptmp_000089"
     CDS             complement(21008..22708)
                     /gene="ybaE"
                     /locus_tag="ACHKNB_RS00445"
                     /old_locus_tag="pgaptmp_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HGU4193678.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22808..23626
                     /gene="cof"
                     /locus_tag="ACHKNB_RS00450"
                     /old_locus_tag="pgaptmp_000090"
     CDS             22808..23626
                     /gene="cof"
                     /locus_tag="ACHKNB_RS00450"
                     /old_locus_tag="pgaptmp_000090"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HGU4193679.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23779..24237
                     /gene="decR"
                     /locus_tag="ACHKNB_RS00455"
                     /old_locus_tag="pgaptmp_000091"
     CDS             23779..24237
                     /gene="decR"
                     /locus_tag="ACHKNB_RS00455"
                     /old_locus_tag="pgaptmp_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HGU4193680.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24267..26039
                     /gene="mdlA"
                     /locus_tag="ACHKNB_RS00460"
                     /old_locus_tag="pgaptmp_000092"
     CDS             24267..26039
                     /gene="mdlA"
                     /locus_tag="ACHKNB_RS00460"
                     /old_locus_tag="pgaptmp_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4193681.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWEQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            26032..27813
                     /gene="mdlB"
                     /locus_tag="ACHKNB_RS00465"
                     /old_locus_tag="pgaptmp_000093"
     CDS             26032..27813
                     /gene="mdlB"
                     /locus_tag="ACHKNB_RS00465"
                     /old_locus_tag="pgaptmp_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HGU4193682.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27994..28332
                     /gene="glnK"
                     /locus_tag="ACHKNB_RS00470"
                     /old_locus_tag="pgaptmp_000094"
     CDS             27994..28332
                     /gene="glnK"
                     /locus_tag="ACHKNB_RS00470"
                     /old_locus_tag="pgaptmp_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HGU4193683.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28362..29648
                     /gene="amtB"
                     /locus_tag="ACHKNB_RS00475"
                     /old_locus_tag="pgaptmp_000095"
     CDS             28362..29648
                     /gene="amtB"
                     /locus_tag="ACHKNB_RS00475"
                     /old_locus_tag="pgaptmp_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HGU4193684.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29696..30556)
                     /gene="tesB"
                     /locus_tag="ACHKNB_RS00480"
                     /old_locus_tag="pgaptmp_000096"
     CDS             complement(29696..30556)
                     /gene="tesB"
                     /locus_tag="ACHKNB_RS00480"
                     /old_locus_tag="pgaptmp_000096"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HGU4193685.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30774..31346
                     /gene="ybaY"
                     /locus_tag="ACHKNB_RS00485"
                     /old_locus_tag="pgaptmp_000097"
     CDS             30774..31346
                     /gene="ybaY"
                     /locus_tag="ACHKNB_RS00485"
                     /old_locus_tag="pgaptmp_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HGU4193686.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPVPTQY"
     gene            complement(31377..31688)
                     /locus_tag="ACHKNB_RS00490"
                     /old_locus_tag="pgaptmp_000098"
     CDS             complement(31377..31688)
                     /locus_tag="ACHKNB_RS00490"
                     /old_locus_tag="pgaptmp_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HGU4193687.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31851..31947
                     /gene="ffs"
                     /locus_tag="ACHKNB_RS00495"
                     /old_locus_tag="pgaptmp_000099"
     ncRNA           31851..31947
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKNB_RS00495"
                     /old_locus_tag="pgaptmp_000099"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            32067..32420
                     /gene="ybaA"
                     /locus_tag="ACHKNB_RS00500"
                     /old_locus_tag="pgaptmp_000100"
     CDS             32067..32420
                     /gene="ybaA"
                     /locus_tag="ACHKNB_RS00500"
                     /old_locus_tag="pgaptmp_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HGU4193688.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32462..34012)
                     /gene="pdeB"
                     /locus_tag="ACHKNB_RS00505"
                     /old_locus_tag="pgaptmp_000101"
     CDS             complement(32462..34012)
                     /gene="pdeB"
                     /locus_tag="ACHKNB_RS00505"
                     /old_locus_tag="pgaptmp_000101"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HGU4193689.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(34176..34646)
                     /gene="ylaC"
                     /locus_tag="ACHKNB_RS00510"
                     /old_locus_tag="pgaptmp_000102"
     CDS             complement(34176..34646)
                     /gene="ylaC"
                     /locus_tag="ACHKNB_RS00510"
                     /old_locus_tag="pgaptmp_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HGU4193690.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34762..35313)
                     /gene="maa"
                     /locus_tag="ACHKNB_RS00515"
                     /old_locus_tag="pgaptmp_000103"
     CDS             complement(34762..35313)
                     /gene="maa"
                     /locus_tag="ACHKNB_RS00515"
                     /old_locus_tag="pgaptmp_000103"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HGU4193691.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(35485..35703)
                     /gene="hha"
                     /locus_tag="ACHKNB_RS00520"
                     /old_locus_tag="pgaptmp_000104"
     CDS             complement(35485..35703)
                     /gene="hha"
                     /locus_tag="ACHKNB_RS00520"
                     /old_locus_tag="pgaptmp_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HGU4193692.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35729..36103)
                     /gene="tomB"
                     /locus_tag="ACHKNB_RS00525"
                     /old_locus_tag="pgaptmp_000105"
     CDS             complement(35729..36103)
                     /gene="tomB"
                     /locus_tag="ACHKNB_RS00525"
                     /old_locus_tag="pgaptmp_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HGU4193693.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(36649..39798)
                     /gene="acrB"
                     /locus_tag="ACHKNB_RS00530"
                     /old_locus_tag="pgaptmp_000106"
     CDS             complement(36649..39798)
                     /gene="acrB"
                     /locus_tag="ACHKNB_RS00530"
                     /old_locus_tag="pgaptmp_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HGU4193694.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(39821..41014)
                     /gene="acrA"
                     /locus_tag="ACHKNB_RS00535"
                     /old_locus_tag="pgaptmp_000107"
     CDS             complement(39821..41014)
                     /gene="acrA"
                     /locus_tag="ACHKNB_RS00535"
                     /old_locus_tag="pgaptmp_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HGU4193695.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            41156..41803
                     /gene="acrR"
                     /locus_tag="ACHKNB_RS00540"
                     /old_locus_tag="pgaptmp_000108"
     CDS             41156..41803
                     /gene="acrR"
                     /locus_tag="ACHKNB_RS00540"
                     /old_locus_tag="pgaptmp_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HGU4193696.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            41931..45293
                     /gene="mscK"
                     /locus_tag="ACHKNB_RS00545"
                     /old_locus_tag="pgaptmp_000109"
     CDS             41931..45293
                     /gene="mscK"
                     /locus_tag="ACHKNB_RS00545"
                     /old_locus_tag="pgaptmp_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HGU4193697.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWCRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            45354..45452
                     /locus_tag="ACHKNB_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /pseudo
     CDS             45354..45452
                     /locus_tag="ACHKNB_RS00550"
                     /old_locus_tag="pgaptmp_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45505..45666)
                     /gene="rsmS"
                     /locus_tag="ACHKNB_RS00555"
                     /old_locus_tag="pgaptmp_000111"
     CDS             complement(45505..45666)
                     /gene="rsmS"
                     /locus_tag="ACHKNB_RS00555"
                     /old_locus_tag="pgaptmp_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HGU4193698.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(45680..46207)
                     /gene="priC"
                     /locus_tag="ACHKNB_RS00560"
                     /old_locus_tag="pgaptmp_000112"
     CDS             complement(45680..46207)
                     /gene="priC"
                     /locus_tag="ACHKNB_RS00560"
                     /old_locus_tag="pgaptmp_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HGU4193699.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            46277..46654
                     /gene="ybaN"
                     /locus_tag="ACHKNB_RS00565"
                     /old_locus_tag="pgaptmp_000113"
     CDS             46277..46654
                     /gene="ybaN"
                     /locus_tag="ACHKNB_RS00565"
                     /old_locus_tag="pgaptmp_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HGU4193700.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            46807..47358
                     /gene="apt"
                     /locus_tag="ACHKNB_RS00570"
                     /old_locus_tag="pgaptmp_000114"
     CDS             46807..47358
                     /gene="apt"
                     /locus_tag="ACHKNB_RS00570"
                     /old_locus_tag="pgaptmp_000114"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGU4193701.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            47487..49418
                     /gene="dnaX"
                     /locus_tag="ACHKNB_RS00575"
                     /old_locus_tag="pgaptmp_000115"
     CDS             47487..49418
                     /gene="dnaX"
                     /locus_tag="ACHKNB_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGU4193702.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    48751..48815
                     /gene="dnaX"
                     /locus_tag="ACHKNB_RS00575"
                     /old_locus_tag="pgaptmp_000115"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            49471..49800
                     /gene="ybaB"
                     /locus_tag="ACHKNB_RS00580"
                     /old_locus_tag="pgaptmp_000116"
     CDS             49471..49800
                     /gene="ybaB"
                     /locus_tag="ACHKNB_RS00580"
                     /old_locus_tag="pgaptmp_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGU4193703.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            49800..50405
                     /gene="recR"
                     /locus_tag="ACHKNB_RS00585"
                     /old_locus_tag="pgaptmp_000117"
     CDS             49800..50405
                     /gene="recR"
                     /locus_tag="ACHKNB_RS00585"
                     /old_locus_tag="pgaptmp_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGU4193704.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            50515..52389
                     /gene="htpG"
                     /locus_tag="ACHKNB_RS00590"
                     /old_locus_tag="pgaptmp_000118"
     CDS             50515..52389
                     /gene="htpG"
                     /locus_tag="ACHKNB_RS00590"
                     /old_locus_tag="pgaptmp_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HGU4193705.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            52570..53214
                     /gene="adk"
                     /locus_tag="ACHKNB_RS00595"
                     /old_locus_tag="pgaptmp_000119"
     CDS             52570..53214
                     /gene="adk"
                     /locus_tag="ACHKNB_RS00595"
                     /old_locus_tag="pgaptmp_000119"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGU4193706.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            53450..54412
                     /gene="hemH"
                     /locus_tag="ACHKNB_RS00600"
                     /old_locus_tag="pgaptmp_000120"
     CDS             53450..54412
                     /gene="hemH"
                     /locus_tag="ACHKNB_RS00600"
                     /old_locus_tag="pgaptmp_000120"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGU4193707.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(54409..55368)
                     /gene="aes"
                     /locus_tag="ACHKNB_RS00605"
                     /old_locus_tag="pgaptmp_000121"
     CDS             complement(54409..55368)
                     /gene="aes"
                     /locus_tag="ACHKNB_RS00605"
                     /old_locus_tag="pgaptmp_000121"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HGU4193708.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            55520..56824
                     /gene="gsk"
                     /locus_tag="ACHKNB_RS00610"
                     /old_locus_tag="pgaptmp_000122"
     CDS             55520..56824
                     /gene="gsk"
                     /locus_tag="ACHKNB_RS00610"
                     /old_locus_tag="pgaptmp_000122"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HGU4193709.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(56957..58633)
                     /gene="ybaL"
                     /locus_tag="ACHKNB_RS00615"
                     /old_locus_tag="pgaptmp_000123"
     CDS             complement(56957..58633)
                     /gene="ybaL"
                     /locus_tag="ACHKNB_RS00615"
                     /old_locus_tag="pgaptmp_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HGU4193710.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(58871..60091)
                     /gene="fsr"
                     /locus_tag="ACHKNB_RS00620"
                     /old_locus_tag="pgaptmp_000124"
     CDS             complement(58871..60091)
                     /gene="fsr"
                     /locus_tag="ACHKNB_RS00620"
                     /old_locus_tag="pgaptmp_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HGU4193711.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDRYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            60309..61961
                     /gene="ushA"
                     /locus_tag="ACHKNB_RS00625"
                     /old_locus_tag="pgaptmp_000125"
     CDS             60309..61961
                     /gene="ushA"
                     /locus_tag="ACHKNB_RS00625"
                     /old_locus_tag="pgaptmp_000125"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HGU4193712.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(61998..62477)
                     /gene="ybaK"
                     /locus_tag="ACHKNB_RS00630"
                     /old_locus_tag="pgaptmp_000126"
     CDS             complement(61998..62477)
                     /gene="ybaK"
                     /locus_tag="ACHKNB_RS00630"
                     /old_locus_tag="pgaptmp_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HGU4193713.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(62681..63475)
                     /gene="ybaP"
                     /locus_tag="ACHKNB_RS00635"
                     /old_locus_tag="pgaptmp_000127"
     CDS             complement(62681..63475)
                     /gene="ybaP"
                     /locus_tag="ACHKNB_RS00635"
                     /old_locus_tag="pgaptmp_000127"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HGU4193714.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            63613..63954
                     /gene="ybaQ"
                     /locus_tag="ACHKNB_RS00640"
                     /old_locus_tag="pgaptmp_000128"
     CDS             63613..63954
                     /gene="ybaQ"
                     /locus_tag="ACHKNB_RS00640"
                     /old_locus_tag="pgaptmp_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4193715.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(64068..66572)
                     /gene="copA"
                     /locus_tag="ACHKNB_RS00645"
                     /old_locus_tag="pgaptmp_000129"
     CDS             complement(64068..66572)
                     /gene="copA"
                     /locus_tag="ACHKNB_RS00645"
                     /old_locus_tag="pgaptmp_000129"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HGU4193716.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            66834..67766
                     /gene="glsA"
                     /locus_tag="ACHKNB_RS00650"
                     /old_locus_tag="pgaptmp_000130"
     CDS             66834..67766
                     /gene="glsA"
                     /locus_tag="ACHKNB_RS00650"
                     /old_locus_tag="pgaptmp_000130"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HGU4193717.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT        17020 a        17112 c        17876 g        15824 t
ORIGIN      
        1 ctatgtgcat tttttatctt acgaatatgc tagttttatc tcactaaata aacgatttgg
       61 tgacatggat gataatgttt caggctaaat gtgtgcttat tacttggacc tttatggcac
      121 tgtatttatt cattaaatgg atgtttggtg gttaattatt atggaaatat atgacagtga
      181 ggacataaag gtatacatgt taactaaaag aatattattt tctgtaatgc tgatagtttc
      241 accaagcgtg gtggccagcg agaaagcgca tgaattgtat gacagcatat acggtggtaa
      301 acctgctccc gatgttataa atacactgca taaaatggct gaatcgggag atatcgacgc
      361 gcagagcctg ttaggctggg aatattatca gcctcgttat gataccaaac ctgatgttca
      421 ggaagcgatt aaatggttcg agttagcggc taaacaaggt gacagagaag ctccgttagc
      481 gctggggggt atctactacg acggtgagca ggtgcgggtg gattatgcca aagcgtatgc
      541 actatttaat caggccgcgc agcacggtgt gaatttagca tggtccaggt tgggcattat
      601 gtacgccaat ggtcagtatg ttgaggtaga ttgtaagaaa gcgaaggaat atcttgataa
      661 aggtgtccac atttatggtg gcccagaaaa ctttctggct acttgtcgaa aagacatgat
      721 tgacagaaaa acggttgacg atacgttacc cgtgattacg gttacccgtt ctgggatgcg
      781 agataatttt ttagataaag gttttcctgc atggatagcc tcttcgccac caccaataaa
      841 ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc cggtaaggag
      901 attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat tagctttact
      961 gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa accggaattt
     1021 gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc aaccattaac
     1081 ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta acgctgctca
     1141 gcccccggct gaggcaaaac ttgccctggc gggtggcgat aataatctaa gccgcgagaa
     1201 tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc gctgaaagct
     1261 gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca agataaaccc
     1321 ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca catagtaggt
     1381 cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact aatctgacaa
     1441 caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aaggcgttgt ttttccagcg
     1501 aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca ataagtgctt
     1561 tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca aatttatcga
     1621 taacagggtt accgcaatca aactgttttt gcccaggata agtaatatca gactgataat
     1681 tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc gctgtttttc
     1741 cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat gagttggttc
     1801 atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag cacactttcg
     1861 gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac ggcttgcgca
     1921 gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg cgcatttttc
     1981 agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac ctctttgatt
     2041 aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa ccgtatgatt
     2101 aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta tctctgatct
     2161 cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttttgtt accacacagc
     2221 agccagcagc gtatgcgagt ccggtaccat aaaatcaacg gacatcatca ccgagagggc
     2281 agtgatggcg atgatagaga agccgaacag cttgcgcgcc cagattctgt catcagcaac
     2341 tttataaccg cgcagagcca tacctaacca ccagacgcta accgccgcgg cgaccaccag
     2401 atatttatat ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg caaaggcgat
     2461 gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg gcaataccgg
     2521 aatgtttgcc gcctggtaat ccttaaagcg gaaaatggcg atggcatagg agtgaggcat
     2581 ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact cgccggttac
     2641 tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca atgtgccgta
     2701 gactgagtgg cgtttcatgt acaggctata gacgccgaca taaaccacaa agcccatcac
     2761 ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc cagcaatacc
     2821 cagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa ccagcacccg
     2881 attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt taaacacaca
     2941 acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg gataatcaat
     3001 gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc caaagatgat
     3061 gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc ttagtgcatc
     3121 atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag gatagcgatg
     3181 attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt ggtattcatg
     3241 tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag gattgttccc
     3301 agaattacgg ccggagaggc agcccctgtc atcaccatcc agaacggaat caccgtcaga
     3361 atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga cgcgccgctg
     3421 tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg aacacacaga
     3481 tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg gtacggttag
     3541 tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag ataagaccgg
     3601 aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg aagccgctgc
     3661 gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc atcccgataa
     3721 atccggcacc aaacaaccag gtcaacgcca gccaggagat aacctggctt ttgttgtttt
     3781 tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc aagaaagttt
     3841 caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct gcggtgccgt
     3901 tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg ctcatcaggt
     3961 agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt tcgtgcgcgt
     4021 gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt agtaatctca
     4081 tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta atccacgtcc
     4141 tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac aatcgccagc
     4201 caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc aatgacgata
     4261 cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt tttatacgct
     4321 tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg cacaacggca
     4381 aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc accccacggg
     4441 tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat ctgaataacg
     4501 aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat cagcatggtg
     4561 tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa gcccagcgca
     4621 tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc gcgtttaccc
     4681 caggtttcat tcagtttgaa accgaaggct ttaggccacc agtaggtcat ccctgcgaag
     4741 cagccgaaga ccacgccgcc gatgatcacg ttatggaagt gcgcaatcag gaacaggctg
     4801 ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat cccgcccacc
     4861 gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac gatgcggccc
     4921 tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc gataatcatt
     4981 gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa gaagtggtgc
     5041 agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag cgaggtataa
     5101 ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc gaaaacaggc
     5161 aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat gtacatcatc
     5221 atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc cagggtcaac
     5281 aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt cgcgcacagt
     5341 gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg catcttcaga
     5401 atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag ctggagactc
     5461 catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag cggtggatag
     5521 gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac cagaatcaca
     5581 ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac gtcacgcgcg
     5641 ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat cgccacgaag
     5701 aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg tggcaggaag
     5761 cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc gtcagcaaaa
     5821 ccacgcagca acatcacaat cgctacgatg atatacatga taccgaggcg tttatggtcg
     5881 acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt gatcaggcca
     5941 acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat aggttcatgg
     6001 aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca acccctttaa
     6061 tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc actgtgctca
     6121 ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt aatcacatcg
     6181 gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc gctaggcgcg
     6241 gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt cgcttttgcg
     6301 acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt catgcctgag
     6361 aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc gttggcgatc
     6421 agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag acgcggaatg
     6481 aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt gttcgccggg
     6541 aaagcgattt cattcacggt agcaatgccc tgttccgggt agatgaagaa ccatttccag
     6601 tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg cttgctaggc
     6661 tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat taagataggt
     6721 accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct gtacttagca
     6781 tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat tgcgggaata
     6841 acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc cagaccaatc
     6901 tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt gcctgcaaat
     6961 aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg acctcaattc
     7021 cacgggatct ggtggcgttt aaagtgtgtg ggcattttac gggaaggtta cattactgta
     7081 aacatgatta aattcctgtt acttggattt ggctgtcagg tcacatatgt tgcaaaacat
     7141 atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg gagtgcagaa
     7201 ggagtttgga cgggaaattg gtataaccaa tctggaataa aacaattgtt taacaaataa
     7261 ttatcattat ggtgacaatt gggtggaggg gaaaataaag actcattagc tgaatcgtgt
     7321 aagaaaaaat agaattcata aattgcgtca ataatttcca aacgctaagc cgcacaaaag
     7381 aacaaatatt attaagcgat actgatgttt atttagccgt aataattacg gcgagtgatt
     7441 actacagcta aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat
     7501 ccagcaaacc accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag
     7561 caggcaggaa agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca
     7621 cggccaacag gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat
     7681 attcggtacg ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa
     7741 ccagcagcaa aataagcccc ggtacggcag cggcgacgga gaatagatag aatgtcgacc
     7801 agccgtgtgc ttcaacaaac caacccgcca cggggccgac ataaactcgc cctacagcgg
     7861 aaagcgctga gagcagggca aattgagttg cggaaaatga cttattacac agcgtcatta
     7921 acagcgcgac aaaggctgat gtgcccatcc cgccacagag gttttcgaaa aagacggctg
     7981 cgcccatgct gtagagatgc ttatcagtaa tcgacagcag ccagtaacca gcgttagacg
     8041 caccttgtaa aatgccgaaa atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa
     8101 tcccaccgta caatgcgccc acaatggtcg ccagtaagcc aagcgttttg ttaaccacgc
     8161 ctacttcacc cgcatcaaac ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg
     8221 cgaatgcgtc gcccagctta tacagcacga taagaagcaa aataagccag gcattattgc
     8281 gaccaaagaa atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa
     8341 tggtgtcggt cggttctggt gcaagcaacg tcgcgataat acaagggatc aacagcgccg
     8401 ccatcaacca gtacatgccc tgccagccca gccatttatc tgccagccac agggctaagc
     8461 cgccggaaac cagcatccct aaacggtaac ccagcacgct gattgccgcg cccgcaccac
     8521 gttcttctgc cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggccg
     8581 agcaaaaagc gatcaccacc gccagtgccg ccatccagcg gagttgggtg ccaggttcga
     8641 gaaaacccat cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc
     8701 gccgccgccc aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa
     8761 aaacgtaagc ctggcctacc agagagaaga aaccaatggt tttgagatca atattctcga
     8821 ccgtcatcca ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac
     8881 ccaggatcag caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca
     8941 tgggcgtcta caggcccggt ttgcaccggg ccagagggca gaaattaacg cgcgttctgc
     9001 ttgataaatt cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca
     9061 ctgttaaccg catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag
     9121 ctggcacgat agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata
     9181 tccgctttgg tcgcgatgtt atagcgcacg ttgccctggg acacgtcagc atacagttgg
     9241 ctaacgatga tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc
     9301 atctgttttt ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta
     9361 acgatttgat tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca
     9421 ttaatgctta cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg
     9481 gaaacttcaa tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac
     9541 gggaagagga tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa
     9601 aaattcccgc catcataaca ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc
     9661 atcatgttga cgaaataatc acccctggta aaagaaacgc tgatgcgagg cctgtgtttc
     9721 aatctttaaa tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat
     9781 ttgttgttaa gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca
     9841 tctcagcgtt gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta
     9901 agggcggcgt tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc
     9961 ccagccggct ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa
    10021 cgttttctga atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc
    10081 gttcatgcgc tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc
    10141 gtctttgcct ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac
    10201 tgtcggcgct tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt
    10261 tgttttgtct ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg
    10321 tgaaaaaata cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat
    10381 aatgccgcgt cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat
    10441 tataagaaca tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc
    10501 gagcactgtg attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct
    10561 tggccgccgt gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga
    10621 gctggtcaac gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat
    10681 gaatatcgtt gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat
    10741 gagccgtaac ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac
    10801 ttatgttccg ggtgaataca agctgggtga agacttcact tactctgtag agtttgaagt
    10861 ttatccggaa gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga
    10921 agtgaccgac gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg
    10981 gaaagaaaaa gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc
    11041 tgtagacggc gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca
    11101 gggtcgtatg atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt
    11161 caccatcgac gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc
    11221 gaaattcgct atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga
    11281 attcatcaaa cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg
    11341 taaaaacatg gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat
    11401 cgaaggtctg gtaaaagcta acgacatcga cgtaccggct gcgctgatcg acagcgaaat
    11461 cgacgttctg cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga
    11521 actgccgcgc gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct
    11581 gggcgaagtt atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat
    11641 cgaagagatg gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa
    11701 caaagaactg atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt
    11761 actggcgaaa gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca
    11821 ggcgtaattt acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg
    11881 tgggcttttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
    11941 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
    12001 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
    12061 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
    12121 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
    12181 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
    12241 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
    12301 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
    12361 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
    12421 ttgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
    12481 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
    12541 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
    12601 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
    12661 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
    12721 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
    12781 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
    12841 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
    12901 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
    12961 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
    13021 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
    13081 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
    13141 gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
    13201 tgctggctga aacgctggcg cgcctgctgg acgtcccgtt caccatggcc gacgcaacca
    13261 cgctgaccga agccggttat gtgggcgaag acgttgaaaa catcattcag aagctgttgc
    13321 agaagtgcga ttacgacgta cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg
    13381 acaagatttc tcgtaagtca gacaacccgt ccattacccg agacgtttcc ggtgaaggcg
    13441 tacagcaggc actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg
    13501 ggcgtaaaca tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt
    13561 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
    13621 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
    13681 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
    13741 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
    13801 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
    13861 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
    13921 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
    13981 tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
    14041 gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa
    14101 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
    14161 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
    14221 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
    14281 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
    14341 gtggtttatc cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt
    14401 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
    14461 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
    14521 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
    14581 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
    14641 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
    14701 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
    14761 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac
    14821 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
    14881 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
    14941 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
    15001 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
    15061 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
    15121 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
    15181 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
    15241 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
    15301 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
    15361 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
    15421 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
    15481 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
    15541 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
    15601 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
    15661 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
    15721 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
    15781 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
    15841 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
    15901 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
    15961 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
    16021 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc
    16081 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
    16141 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
    16201 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
    16261 gaaaaacgtg acatccacgt ccacgttccg gaaggtgcga cgccgaaaga tggtccgagt
    16321 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
    16381 gatgtggcaa tgaccggtga gataactctg cgtggtcagg tactgccgat cggtggtttg
    16441 aaagaaaaac tactggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
    16501 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
    16561 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag
    16621 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
    16681 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
    16741 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
    16801 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
    16861 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
    16921 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
    16981 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
    17041 cggtaaagag atcaccatcg ctgccgctaa agtaccgagc ttccgtgcag gtaaagcact
    17101 gaaagacgcg gtaaactaag cgttgtcccc agtggggatg tgacgaagtt caagggcgca
    17161 tctactgatg tgcctttttt atttgtattc ggtgactttc tgtgtcttgt gggctgacaa
    17221 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    17281 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    17341 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    17401 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    17461 agccgtgggc agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg
    17521 ggcgatcaat actccgagct ggcagcgaac gaaggctata tgaaaaccct gcgtcaacag
    17581 gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg
    17641 ggtatcagtg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    17701 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    17761 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc
    17821 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    17881 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    17941 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    18001 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    18061 gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc
    18121 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    18181 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt
    18241 aacggcggcg atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct
    18301 ggtctgaaag aaaaagggca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    18361 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    18421 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa
    18481 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgctggc
    18541 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    18601 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    18661 atcaactctg acatcatcac cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag
    18721 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcattggtt
    18781 cagcacaaca aagctcaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    18841 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    18901 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    18961 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    19021 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    19081 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    19141 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    19201 aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    19261 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    19321 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt
    19381 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac aacggtagaa
    19441 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    19501 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    19561 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    19621 tttaaaacgg tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    19681 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    19741 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    19801 caaacacaaa tcaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc
    19861 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    19921 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    19981 cgcccagcgg tattaagtga cctgttaact attaccagtc agttgcagca attaaacggt
    20041 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    20101 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    20161 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    20221 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    20281 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    20341 cgcacaatga ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg
    20401 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca
    20461 catcagcggc gtttcaaaac gaatatcttt cgccatgccc aaactgacgg catggtttag
    20521 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc
    20581 ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    20641 aatattacgc cctgggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg
    20701 caccggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc
    20761 aagaacctta tgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    20821 tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    20881 tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt
    20941 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    21001 cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    21061 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    21121 gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc
    21181 ggttttcctc atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg
    21241 ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat
    21301 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg
    21361 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    21421 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca
    21481 gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg ccagtacgga atagtccagc
    21541 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    21601 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    21661 ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc
    21721 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctgtttccca
    21781 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    21841 gcaaatggta ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc
    21901 gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    21961 ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat
    22021 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    22081 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    22141 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    22201 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    22261 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    22321 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    22381 gctgtaacag agtgcgcagc tcacctgggg ccagttgcgc cagctccagc acatcttgct
    22441 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    22501 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    22561 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    22621 ccagttcgct gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat
    22681 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    22741 ctattacttc cgtatatatc aggtgatact caatcaccat taaccgtgtc acagagtgga
    22801 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    22861 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    22921 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    22981 ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    23041 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    23101 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    23161 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    23221 gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt
    23281 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    23341 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    23401 acatttaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    23461 aatgttaggc agcgtcggta gcgggtttat tatgggcaat gcgatgccgc aactgcgcgc
    23521 ggagctcccg catttaccgg tgattggaca ttgccgaaat caggctgtct ctcactattt
    23581 gacgcactgg ctggactatc cacatctacc ttattccccc gaataacgag atcccttcca
    23641 gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    23701 aaaccgcaca gaataaattg tcgtgatttc acctttaaaa tagaattaaa agagaaaaaa
    23761 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
    23821 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct
    23881 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    23941 ctggatccgg aaaaaatagg cctcggcctg accgcttttg tgctgataaa aacgcaacat
    24001 cacagcagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    24061 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    24121 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    24181 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    24241 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    24301 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    24361 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    24421 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatc
    24481 tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    24541 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    24601 gcacgggtga tctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgccgccg
    24661 gagaaggggt gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga
    24721 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
    24781 cgatcatgat caagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg
    24841 cgttttctag tcttaatgac cgcacccagg aaagcctcac cagtatccgc atgatcaaag
    24901 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    24961 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccaaccatc tatatcgcga
    25021 ttggtatggc gaacttgctg gcgattggcg gcggtagttg gatggtggcg cagggcagtt
    25081 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    25141 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcgtac agccgtattc
    25201 gtgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    25261 gtggcgaact tgatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    25321 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    25381 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    25441 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    25501 tggcggtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt
    25561 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    25621 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga agtgggcgag cgcggcgtga
    25681 tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg agcgttatta gtcaacgcgg
    25741 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
    25801 tgcataacct gcgtcagtgg gaacagggaa gaacggtaat catcagtgcc catcgccttt
    25861 cggcactgac ggaagccagt gaaattattg tgatgcagca cggacatatc gcccagcgtg
    25921 gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    25981 aactggaggc ggcgctcgac gacgctccgg aaattcgcga ggaggccgtc gatgcgtagt
    26041 tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcgtaaa
    26101 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg
    26161 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg
    26221 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
    26281 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    26341 gtgatggatg ccgcattgcg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    26401 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    26461 gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
    26521 gactggcgaa tggcgctggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg
    26581 atataccagc gttacagcac gccgattgtc cgccgtgtgc gcgcctatct agcggatatc
    26641 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag
    26701 gcgcgatttg gcgaacgtat gggggaggcc agtcgttcac actatatggc caggatgcaa
    26761 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    26821 ctttgtggct tgttgatgct gtttggcttc tccgctagcg gcaccattga agtgggcgtg
    26881 ctgtatgcgt ttatcagcta tcttgggcga cttaacgaac cattaatcga actgaccacg
    26941 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    27001 ggaccgcgcc agcaatatgg caatgatgag cgcccgttac agagtggcac catcgaagtc
    27061 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    27121 gtgccttcgc gtaactttgt ggcactggtc gggcataccg gcagtggcaa aagcaccctc
    27181 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    27241 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    27301 ccggttgtgc tggcggatac cttcctcgcc aacgtgacgc tggggcggga tatctccgaa
    27361 gaacgcgtct ggcaggcgct ggaaactgtg caactggcgg agctggcgcg tagcatgagc
    27421 gacggtattt acacgccgct gggcgagcag gggaataatc tctcagttgg gcaaaagcaa
    27481 ctgctggcac tggcgcgcgt gctggtcgag acgccgcaaa tcctgatcct tgatgaggca
    27541 accgccagca ttgactccgg gactgaacag gcgattcaac atgctctggc ggcggtgcgt
    27601 gaacatacca cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc
    27661 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    27721 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    27781 agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    27841 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    27901 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    27961 gaaccattac cgaattctga ccggagggga tctatgaagc tggtgaccgt gataatcaaa
    28021 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    28081 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    28141 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcgattgc tgatgaccaa
    28201 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    28261 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    28321 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    28381 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    28441 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    28501 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    28561 tgacgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    28621 cgctggcgtt tggtgagggc aacaacttct tcggcaacat taactggttg atgctaaaaa
    28681 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgtg gcgtttcagg
    28741 gctcgtttgc ctgcattacc gtcggcttga tcgttggggc gctggcggaa cgaatccgct
    28801 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    28861 atatggtgtg gggcggtggt ttgctggctt ctcacggtgc gctggatttc gcgggtggca
    28921 cggtggtgca tattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    28981 gcgtgggctt cggcaaagag gcgtttaaac cgcacaacct gccaatggtc ttcaccggta
    29041 ctgccattct ctatattggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    29101 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    29161 ggatcttcgg tgaatgggcg ctgcgtggta agccttctct gctgggagcg tgttctggcg
    29221 cgattgccgg tctggtcggc gtgacgccag cgtgtggcta cattggggtt ggcggcgcgt
    29281 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttactatg ctcaaacgct
    29341 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    29401 gctgtatcat gactgggatt tttgccgcca gttcgctggg tggcgtgggc ttcgctgaag
    29461 gtgtgacgat gggccatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    29521 ggtccggtgt ggtggcattt attggctaca aactggcgga tctgacggtt ggtctgcgtg
    29581 taccggaaga gcaggagcga gaagggctgg atgtcaacag tcacggcgag aatgcctata
    29641 acgcgtaaca agcactgcaa aaaatagccg gacggtttta cctccggcta tttttttaat
    29701 tgtgattacg catcacccct tcctgaacgg tcgaggcaac cagcacgccg tcctgggtat
    29761 aaaactcacc gcgcacaaag ccgcgtgcgc tggacgccga ggtgctctcc acgctataca
    29821 gcagccattc attcaaatta aacgggcgat ggaaccacat ggaatggtca atggtggcaa
    29881 tctgaatccc cggttcgaga aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt
    29941 taagatcaga agcgtaaccg agcagatact gatgaacgcg caggtcatcc ggcacgctac
    30001 catttgcgcg gatccacacc tgacgatgtg gttctgcgac gtgacctttc agtgggttat
    30061 gaaactccac cggacggact tccagcggac gatcgcagat gaatttatct ttaagcactg
    30121 gcggcagcag gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg ccatcaggcg
    30181 ctggcgcgga cggcattgtt ttttgatgtt cgaaacccgc ttctggtgcc tggaaagagg
    30241 ccgtcatata aaaaatcggt ttgccgtttt gaatagcagc aacccggcgg gcgctgaagc
    30301 tgttaccgtc acgcagcgtt tcgacatcat aaataatcgg cttcttacta tcgccagggc
    30361 gaagaaagta gctgtgaaac gaatgtacca gccgctcttc agggacggtc tcttttgcag
    30421 catacaaggc ctgacccacg acctggccgc caaacacctg gcgtaaacct aaatcttcac
    30481 tctggccgcg aaagagtcct tcctcaattt tttccagatt taacaatgtc agtaaatttt
    30541 ttagcgcctg actcatataa ctctccagta acaaagctgc cgcagcaagc caaagtgagt
    30601 tgagtataac gcaaatttgc tactggtccg atgggtgcaa tggtctgaat tacgggctaa
    30661 ttacaggcag aaatgcgtga tgtgtgccac acttgttgac gttactattt tgttaaccac
    30721 tcttccggcg aggaaagtta gcccgctggt gcattgataa taaggagaaa tgaatgaaac
    30781 tcgtgcacat ggccagtggt ttagcggttg cgattgcgtt ggcggcttgc gcagataaaa
    30841 gcgcggatat tcagacgcca gccccggctg caaatacgtc tatttcagca acacaacaac
    30901 ctgctatcca gcaaccgaat gtctccggta ccgtctggat ccgtcagaaa gtcgcactgc
    30961 cgcctgatgc tgtgctgacc gtgacacttt ctgacgcgtc gttagccgat gcaccgtcaa
    31021 aagtgctggc gcagaaagcg gtgcgtactg aaggtaaaca gtcaccattc agctttgttc
    31081 tgccatttaa cccggcagat gttcagccga acgcgcgtat tctgttgagt gcggcgatta
    31141 ccgtgaatga caaactggta tttattaccg ataccgttca gccggtgatc aacaagggcg
    31201 gaactaaagc cgacctgaca ttggtgccgg tgcagcaaac cgccgtgccg gttcaggcca
    31261 gcggtggtgc aacgactacc gtaccttcga cttcaccaac tcaggtgaat ccgtcttcgg
    31321 cagttccagt tcctacgcaa tattaagcga cgttaaccct ctccgcccgg agagggttag
    31381 tagttccagc gataacgctg caagtcgatt tgcccgcttc ccgataccat cacaccttct
    31441 gccagtaatg cctgtcgctg acgctgtaaa tccggtccgg ttagcgaaat tgtgccgtgg
    31501 cgattaacca cccggtgcca gggtaaggtg ctgccttcag ggagacgctt taacacaccg
    31561 cccacctggc gcgcggcgcg gggcgatccc gccagtttcg ccacatcacc gtaagtggtg
    31621 acatagcctt cgggaatagc ggcgacgatt tgccagacgc gttggggaaa tgaatcttct
    31681 ttttccatct tttcttcctg aggtaatttt tcagcataat ctggaaaaac gcccgagtga
    31741 agtcgcattg cgcaagaaac cagcatctgg cacgcgatgg gttgcaatta gccggggcag
    31801 cagtgataat gcgcctgcgc gttggttctc aacgctctca atgggggctc tgttggttct
    31861 cccgcaacgc tactctgttt accaggtcag gtccggaagg aagcagccaa ggcagatgac
    31921 gcgtgtgccg ggatgtagct ggcagggccc ccacccattt ctgcctccca ccgtttcgtc
    31981 aaaaaacccc aacatggcta aactttaacc acaactgacg tcgcaagaat tgtctggctg
    32041 cgcagtacgc ttcggaggta tgtctgatga agtatgttga tggttttgtg gttgccgttc
    32101 ctgccgataa aaaggatgcc tatcgggaaa tggccgctaa ggctgcgcca ttgtttaaag
    32161 agtttggcgc gcttcgtatt gtcgaatgct gggccagcga tgtaccggat ggcaaagtga
    32221 ccgattttcg tatggcggtg aaagcggaag agaatgaaga ggttgtcttt agctggattg
    32281 aatacccttc aaaagaggtc cgcgacgctg ctactcaaaa gatgatgtcg gacccacgga
    32341 tgaaagagtt cggcgagtcc atgccgtttg acggcaagcg aatgatctat ggcggattcg
    32401 agtcaatcat cgacgaatag gtagcgtgac gggctgtgcg gcgtgccgtc agcccaaaaa
    32461 ttcacaaatg ttgctcggcc cagcgtaaga aatcttcttt cggtaatgcc ttgctgtaga
    32521 gccagccctg accgtagtgc acgccatgct ggcgtagcca ctcttcttgt ttactggttt
    32581 cgattccctc cgctaccatt ttcagtttca gtgtttttgc catttcgatg atatgcggcg
    32641 tgacattttt atattccagc gcatcaacga aagatttatc gatcttcaga atgtcgacat
    32701 ccagatcctg taaataactt aaacttgaat atcccgtacc aaaatcatca agataaattt
    32761 catggcccgc ctcccggtag cgagaaatta tcggggcgct ggttttcgga tcggcaaact
    32821 cgcgttcagt gagttcaagc gcgatctgtc tgggattaac ctgatagtga ttgatcattt
    32881 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    32941 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaagacg cttcttataa
    33001 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    33061 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    33121 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    33181 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    33241 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    33301 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    33361 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    33421 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    33481 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    33541 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    33601 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    33661 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    33721 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    33781 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    33841 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    33901 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    33961 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    34021 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    34081 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    34141 ggcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    34201 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    34261 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    34321 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    34381 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    34441 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    34501 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    34561 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    34621 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    34681 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    34741 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    34801 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    34861 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    34921 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    34981 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    35041 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    35101 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    35161 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    35221 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    35281 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    35341 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    35401 gatcacatag ggtatgtctt catggtgaaa aagtataaaa ttcttaataa acagccggtt
    35461 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
    35521 gtcgtatagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
    35581 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
    35641 aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga
    35701 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
    35761 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
    35821 gcatattaat accataactg ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
    35881 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa
    35941 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
    36001 catgattgct ttcttcaagg tttgcaagac agtcatgata cagggtttca cagagaaact
    36061 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
    36121 gcggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct
    36181 ctcgtttgtt agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa
    36241 aattacaatt aggaaggaag cgggacagga ctcacttttt catactagca ctaacccgca
    36301 gcaggtaaaa gcagtttgct cgaactactc ataacatttc aggattatcg ccagatgaaa
    36361 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
    36421 gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta aatctattag
    36481 cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat acgtggttaa
    36541 tactggtttt cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt
    36601 tatgcataaa aaaggccgct tacgcggcct tagtgattac atgttgtatc aatgatgatc
    36661 gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac
    36721 aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac
    36781 accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat
    36841 aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcaccgc
    36901 atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc
    36961 gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacctggaa
    37021 gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag
    37081 cggaacgacc agcataacgg agaacggaat cgaccagctc tcgtacagtg ctgccagaca
    37141 caggaacacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagagacg
    37201 ttcctgatag gacatccccg tccagtcata gcccacaccg gtaggcagtt tgctcgccag
    37261 ttgttccatt agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat
    37321 ttccatagat ggcaggccgt tgtaacgttc cagacgcggc gaaccgtact cccaacgaga
    37381 agaggagaac gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc
    37441 gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg
    37501 accgcggtcg ataaagtcgt tcacatagct accaccccat gcagcgccca gagtggtgtt
    37561 aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa
    37621 ctgcggggta tcttccagac cgtttggacg tacgctggtc aacatatcag ggtgcttcgc
    37681 tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg
    37741 gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt
    37801 aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatagtaat
    37861 cgcttcaact ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc
    37921 aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc
    37981 aacgttgttc ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt
    38041 acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc
    38101 atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat
    38161 cagatacagc accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta
    38221 gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc
    38281 cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat
    38341 caacgctacc agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata
    38401 gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat
    38461 cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg
    38521 cggcaaacct tcttccgcca taacacgctc aacgttttct accacaacga tagcgtcatc
    38581 caccaacaag ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc
    38641 aaaggcggca aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat
    38701 caacgtcgcg cggaagttct gcaggaacag atacatcacc aggaacacga ggatgatcgc
    38761 ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta
    38821 cgggtaaaca attttcagac ctgacgggaa gaacggttcc atcttcgcca gttcagcacg
    38881 gattgccgca gcggtatcca gcgcgtttgc accggtcgcc agcttgatcc ccagaccgga
    38941 agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt
    39001 cgccacatca cgcagcagta cgcgggaacc atcctgattc actttcagca ggattttgcc
    39061 gaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc
    39121 tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat
    39181 ggcggtaatg acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca
    39241 gatacgcatc gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct
    39301 gatggcatct ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc
    39361 atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac
    39421 cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac
    39481 ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt
    39541 acccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
    39601 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
    39661 ggagatcgtt actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat
    39721 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
    39781 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
    39841 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
    39901 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
    39961 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtctcca
    40021 ctttgtcatc cgcgccaact accagtacgg tggcatcgcc acgcggcgta cgggttacgc
    40081 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttcaagacgt gcgcgcacga
    40141 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
    40201 cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
    40261 tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
    40321 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga
    40381 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
    40441 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agaggtgact ttggtgtaag
    40501 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
    40561 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
    40621 gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
    40681 gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag
    40741 agacacctgc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
    40801 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
    40861 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
    40921 gttgggcctg tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga
    40981 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
    41041 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
    41101 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
    41161 acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg
    41221 tcttttctca cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg
    41281 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
    41341 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
    41401 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
    41461 agaagaacga cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
    41521 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
    41581 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
    41641 ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
    41701 gcaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta
    41761 tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca
    41821 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
    41881 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
    41941 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
    42001 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
    42061 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
    42121 acaaactggt gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgca
    42181 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc
    42241 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
    42301 ttctgagtac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
    42361 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
    42421 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
    42481 gtcgtctgga tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa
    42541 tgcaggctca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag
    42601 ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg
    42661 cccgtctgga gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt
    42721 taactgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
    42781 atccgctggt gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
    42841 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
    42901 gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
    42961 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
    43021 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg
    43081 atgcactttt ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag
    43141 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
    43201 atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc
    43261 agcagcagtt aatgagtgtg tcgaaaaacc tgaaatccat cctgactcag caaatctttt
    43321 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
    43381 aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt
    43441 ttatcgcttt cctcgccggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc
    43501 tgggctggct gaaagcgtat caacagaaac tggcttccgc tgtgggttcc ctgcgtaacg
    43561 atagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt
    43621 gcctgattat tctcgcggtt ggcctgattc tgttgaccat gcagctcaac atcagcgaac
    43681 tgctatggtc gttcagtaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga
    43741 aggtactgga gaaaaatggc gttgccgtgc gtcacttcgg catgccggag cagcagacca
    43801 gccactggtg tcggcaaatt gtccgcatca gtctcgcgtt gctgcctatc catttctggt
    43861 ctgtggtagc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga
    43921 ttttcttcaa cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc
    43981 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctatcg attatcccga
    44041 ttgcgttgat ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc
    44101 gttggattga aaccgtttat ttggtgatca tctggaacct gctgtaccag acggtattgc
    44161 gcggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
    44221 atctggtgaa agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg
    44281 agcaagttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
    44341 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca
    44401 cgctctggca ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
    44461 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
    44521 ctggtttact ggaagtgctg gtgctctcgc gactgaatat gcgtcagggc gcgtcgtatg
    44581 ccattactac catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat
    44641 cgctgggcgt ctcttgggat aaactccagt ggctggccgc agcattatcc gtaggtcttg
    44701 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac
    44761 gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
    44821 tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
    44881 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg
    44941 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
    45001 aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg
    45061 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
    45121 gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca
    45181 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
    45241 cggaagtcaa acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa
    45301 aggtggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat
    45361 gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg
    45421 gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg
    45481 acatttagcg gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt
    45541 ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt
    45601 ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag
    45661 tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca
    45721 gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt
    45781 gctgttccac gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac
    45841 gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg
    45901 gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca
    45961 gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg
    46021 catgacgaag cgcagccaga ttatcgcccg cctcatcgag acaagcctgc agtgttgtcg
    46081 cacgggtttg aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg
    46141 gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg
    46201 ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa
    46261 gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt
    46321 gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc
    46381 ggcctggtgc tttgcccgtt cttccccgcg ctttcacgct tggttgctgt accgctcatg
    46441 gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc
    46501 gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc
    46561 atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat
    46621 tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat
    46681 tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa
    46741 caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat
    46801 acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt
    46861 caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg
    46921 aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc
    46981 aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg
    47041 ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa
    47101 acttacgacc tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg
    47161 ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc
    47221 gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg
    47281 ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc
    47341 ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag
    47401 atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc
    47461 accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc
    47521 cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct
    47581 tgtcgttagg gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa
    47641 cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc
    47701 cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg
    47761 aaatcgacgc cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc
    47821 agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt
    47881 cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt
    47941 ttctgctggc gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc
    48001 aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca
    48061 acgaagaaca tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag
    48121 gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg
    48181 tttcaaccca ggcagtcagt gcgatgctgg gtacgcttga cgacgatcag gcgctgtcgc
    48241 tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg
    48301 ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta
    48361 ttgcgatggt acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc
    48421 ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc
    48481 tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga
    48541 cgctgctgcg cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccac
    48601 gccagtcctt tgcgcccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc
    48661 aaccgcaatc agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg
    48721 tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg
    48781 aaccggcagc cgctacccgc gcgcggccgg tgaataacgc tgcgctggaa agactggctt
    48841 cggtcaccga tcgcgttcag gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca
    48901 aaaaagaagc gtatcgctgg aaggctacca ctccagtgat gcagcaaaaa gaagtggtcg
    48961 ccacgccgaa ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga
    49021 agctggcggc agaagccatt gagcgcgacg cgtgggcggc acaggtgagt caactttcgc
    49081 taccaaaact ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag
    49141 tatgtctgca tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac
    49201 tggctgaagc gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg
    49261 ataatcccgc ggtgcgtacg ccgctggagt ggcgtcaggc gatatacgaa gaaaaacttg
    49321 cgcaggcgcg cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg
    49381 atgcggagct ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg
    49441 tcatccttaa cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg
    49501 atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag
    49561 ctggaagtta ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac
    49621 aactgccgtc gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa
    49681 gacctggtgg ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa
    49741 aaaatggcct ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga
    49801 tgcaaaccag cccgctgtta acacagctta tggaagcact gcgctgtctg ccgggcgttg
    49861 gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga
    49921 tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc
    49981 gcactttcac cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg
    50041 gtcaaatctg tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt
    50101 tttcaggccg ttattttgtg ttgatggggc acctgtcacc gctggacggc atcggtccgg
    50161 atgatatcgg ccttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga
    50221 tcctcgccac caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt
    50281 gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg cgtaccggtt ggcggcgaac
    50341 tggaaatggt cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt
    50401 tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca
    50461 tctctccctc atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa
    50521 ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat
    50581 tctctctatt ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg
    50641 gcggacaagc tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa
    50701 ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc
    50761 gtggggatga cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc
    50821 aaatcattcc tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag
    50881 tttggtgttg gtttctactc tgcgtttatc gtggccgaca aagtgaccgt gcgtactcgc
    50941 gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat
    51001 accgttgccg acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa
    51061 ggcgaagacg agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac
    51121 catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc
    51181 tcctgggaga aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc
    51241 gatgaagagt acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc
    51301 tggagccaca accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc
    51361 caggctccgt gggatatgtg gaaccgtgat cataaacacg gcctgaaact gtacgtccag
    51421 cgtgtgttca tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt
    51481 ggtctgattg actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc
    51541 acggtaacgc gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa
    51601 ctggcgaaag acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg
    51661 aaagaaggtc cggcggaaga tttcgcgaac caggaagcga tcgccaaact gctgcgtttt
    51721 gcttctaccc ataccgattc ttctgcgcag accgtatctc tggaagacta cgtttcccgc
    51781 atgaaagaag ggcaggagaa aatctactac atcaccgcag acagctatgc ggcagcgaag
    51841 agcagcccgc acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac
    51901 cgcatcgatg agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg
    51961 gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagtgcgaaa
    52021 gaagcggaga aagcactgac tccgttcatc gaccgtgtga aagccctgct cggcgagcgc
    52081 gtgaaagatg tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac
    52141 gcggacgaaa tgagcactca gatggctaaa ctgttcgctg cggcgggcca gaaagtgcca
    52201 gaagtgaaat acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat
    52261 actgaagatg aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg
    52321 gcagaacgcg gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg
    52381 gtttcctgat gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct
    52441 gcactttccg caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc
    52501 aatcgcctgt tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg
    52561 attttcgcaa tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct
    52621 cagttcatca tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct
    52681 gcggtcaaat ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa
    52741 ctggtcaccg acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc
    52801 cgtaatggtt tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa
    52861 gaagcgggca tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt
    52921 gatcgtatcg taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc
    52981 aatccgccga aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa
    53041 gacgatcagg aagaaaccgt gcgtaaacgt ctggttgaat accatcagat gactgcaccg
    53101 ctgatcggct actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac
    53161 ggcaccaagc cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaattcgaa
    53221 agcgcgcacg gacagtcccc tcgccccctc ggggagaggg ttagggtgag gggaacaggc
    53281 ccgcacaagc aaacttatca gcaatctcag gccggatatt cattcggcct tttacaaaaa
    53341 cctcaatctg cgctattcct ttttctgatt tgacctctca cagcaattag ttcttcttcc
    53401 tcacttttcc gctacaatta tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac
    53461 taaaaccggt atcctgctgg caaacctggg tacgcccgat gcccccacac ctgaagcggt
    53521 aaaacgctat ctgaaacaat ttttaagcga cagacgcgtg gttgatacct cacggttgtt
    53581 atggtggccg ttgctgcgcg gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct
    53641 gtacgcctcg gtctggatgg aagacggctc gccgctgatg gtttacagcc gccagcaaca
    53701 gcaggcgctg gcacaacgtt taccggagac gcccgtagcg ctgggcatga gctacggctc
    53761 gccatcactg gaaagcgccg tcgatgaact cctggcagag catgtagatc atattgtggt
    53821 gctgccgctt tatccgcaat tctcctgttc tacggtcggt gcggtatggg atgaactggc
    53881 acgcattctg gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg attacgccga
    53941 taaccacgat tacattaatg cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg
    54001 cgaaccggat ctgctgctgc tctcttatca tggcattccc cagcgttatg cagatgaagg
    54061 cgatgattac ccgcaacgtt gccgcacaac gactcgcgaa ctggcttccg cactggggat
    54121 ggcaccggaa aaagtgatga tgacctttca gtcgcgcttt ggccgggaac cctggctgat
    54181 gccttatacc gacgaaacgc tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt
    54241 gatgtgcccg ggctttgctg cggattgtct ggagacgctg gaagagattg ccgagcaaaa
    54301 ccgtgaggtc ttcctcggtg ccggcgggaa aaaatatgaa tatattccag cgcttaatgc
    54361 cacgccggaa catatcgaaa tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc
    54421 ggtaaagaac tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca ttcgtgaata
    54481 atgcaaaaag gcatgcagcg tacctgggta gagtttgaac tcacagggct gctgatgcgc
    54541 cgctaacgtc tggtaaagca gacggctgtc atccagcagc ggatcgaact ccgccccggc
    54601 aataaaacag ggcggaactt cgcgagtgag atcattatta aacagacagt aatacggtga
    54661 ctcgcggtcc gcgtcgttgc ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt
    54721 taagccatcc cagacaccgc ccaacagacg acgagtcacg gaatcccgta atccgtaaag
    54781 cccataccac agcaaaacac ccgcaatttt accgcaatcg atctgtttat cacgcaacca
    54841 caacgcactg gcgagcgcca gcatggcacc tgcggaatca ccggcaaagc caatgcggga
    54901 catattgatt tgataatcct ccgcctgctg gtggaaataa caacaagcag ccacaatttc
    54961 ctctatcgct tgcggaaaac gcgcttcagg tgaaaggggg taatcaatac caatcaccgt
    55021 acattggctg tagcttgcca gcaggcgcat gatgcgatcg tgggtatcga gattgccgag
    55081 aataaaaccg cctccatgca aataaaatag cgtcgccggg ctatctggct gcggacaaaa
    55141 gagacgtgtt tccacctgcc catattttgt tggaaccatg taagctctgg ttgccatttc
    55201 tggagcgccc gcattccaga atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat
    55261 cgttcccgtt gcgggccagg acggtaaatc cgactgaaga gtattcacaa cggtcttcat
    55321 ttcagcagaa ataaggtcca gaacaggtag tttgttttcc ggcttcatac aaaaactcct
    55381 ttcaaattac gtcattgtaa ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat
    55441 tgaaactggc tgcgtctcgc gcgctcccgt cagattgtgt taacattcgc cgctcagtta
    55501 accacccgta aaaacaacca tgaaatttcc cggtaaacgt aaatccaaac attacttccc
    55561 tgtaaatgca cgcgatccgc tgcttcagca attccagcca gaaaacgaaa ccagcgccgc
    55621 ctgggtcgta ggtattgacc agacgctggt cgatattgaa gcgaaagtgg atgatgaatt
    55681 cattgagcgt tatggattaa gcgccgggca ttcactggtg attgaggatg acgtagccga
    55741 agcgctttat caggagctaa aacagaaaaa cctgattacc catcagtttg cgggtggcac
    55801 cattggtaac accatgcaca actactcggt gctcgcggac gaccgttcgg tgctgctggg
    55861 cgtcatgtgc agcaatattg aaattggcag ttatgcctat cgttacctgt gtaacacttc
    55921 cagccgtacc gatcttaact atctacaagg cgtggatggc ccgattggtc gttgctttac
    55981 gctgattggc gagtccgggg aacgtacctt tgctatcagt ccaggccaca tgaaccagct
    56041 gcgggctgaa agcattccgg aagatgtgat tgccggagcc tcggcactgg ttctcacctc
    56101 atatctggtg cgttgcaagc cgggtgaacc catgccggaa gcaaccatga aagccattga
    56161 gtacgcgaag aaatataacg taccggtggt gctgacgctg ggcaccaagt ttgtcattgc
    56221 cgagaatccg cagtggtggc agcaattcct caaagaccac gtttctatcc ttgcgatgaa
    56281 cgaagatgaa gccgaagcgt tgaccggaga aagcgatccg ttgttggcat ctgacaaggc
    56341 gctggactgg gtagatctgg tgctgtgcac cgccgggcca atcggcttgt atatggcggg
    56401 ctttaccgaa gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc
    56461 ggaattcaac cagtatgagt ttagccgcgc catgcgccac aaggattgcc agaatccgct
    56521 gcgtgtatat tcgcacattg cgccgtacat gggcgggccg gaaaaaatca tgaacactaa
    56581 tggagcgggg gatggcgcat tggcagcgtt gctgcatgac attaccgcca acagctacca
    56641 tcgtagcaac gtaccaaact ccagcaaaca taaattcacc tggttaactt attcatcgtt
    56701 agcgcaggtg tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc agcattcacc
    56761 tcgtttaacg cgcggcttgc cggagcgtga agacagcctg gaagagtctt actgggatcg
    56821 ttaagttatc gtcggttcgt aggccagata aggcgttcac gccgcatctg gcatttggct
    56881 ctcgatgcct gatgcgacgc tggcgcgtct tatcatgcct acatattttt catattttac
    56941 atccggcaac caccgtttac cccgtcacca cctcacccgc cggtggcgtt tccagcagtt
    57001 ccagcatggt acgggcgatt tcacgctcgc ccatcactac ctgattcgca ccacgttcgg
    57061 tgatatacgc cacttcatcg tcataatggg cgcgggcaat aatctcaata tccggatttt
    57121 tcgcgcgggc agatgccaca atctcacccg cttcataacc gttgggaatc gtcaggatca
    57181 gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttcgcc gcattgccca
    57241 atactgcgcg gaccccgcgc tctcgcagct catcaacacg ggttcgtgac gtctcaatca
    57301 ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct acacgaccgt
    57361 aacccaccag tagcgcatgg ttgcaaatat ccactgggat ctgcttctct tcttcgattg
    57421 cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc tccagtagtg
    57481 cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc aggttttgtc
    57541 cggcctgcgg cagtaaattc aatgccattc ccagtcccgc caggataaac gcgaactcac
    57601 caatctgcgc caggctggcg gcgatggtta atgccgtacg ttgggagtga ccaaacagtc
    57661 gcaccaggaa aaatgcggct aacgacttac caaacagaat aatcgccagc gtcgccagca
    57721 ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg gagacaaaaa
    57781 acagcaccgc aaacgcgtcg cgcaatggca gcgtatcgtg ggcggcacgg tgactcagtt
    57841 cagactcgtt cagtaccatc ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca
    57901 gctctaccgc accaaaggca atccctaacg ccagcgccag caccgacagg gtaaacagct
    57961 cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg caccagacgg cgacctacca
    58021 gcatcataat ggcgataaat gcgatcactt tgccgatggt gatccccata tcgactgcaa
    58081 gagtggcaaa gcccacatcg ccctgttcca tcattcctgc cactgcgggc agcaacacca
    58141 gcgtcagaac cattaccagg tcttccacaa tcaaccaacc gatggcgatt tgcccacgct
    58201 gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg
    58261 aaagacataa accgaacacg ataccggtca ttaacgacca gcccagcacg gcggagagcg
    58321 ccatacccag cagcgtcgcc acggctatct gggcgatcgc gccgggaatg gcgatggact
    58381 ttaccgccat caaatccttg agcgaaaaat gcaaaccgac gccgaacatc agcaaaataa
    58441 cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg
    58501 gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt agtttattgg
    58561 ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg ataagcgggg
    58621 tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg gccgggaaaa
    58681 ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgttcatga ataattattg
    58741 aattttgcag aaaaatggaa ttagctgcaa aaaaagcacg gatcggaaaa cagaaggcgg
    58801 tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt tgcctttggc
    58861 tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac atccccaata
    58921 gtggcaggaa agcacagatt ttatagacta actcgatact ggtgtgatcg gcgataagcc
    58981 ccagaactgc cgctcccaga cctcccatac caaaagcaaa accgaaaaag agtccagaaa
    59041 ccataccgat acgtcccgga agcagctcct gagcgtaaac cagaatggca gagaatgccg
    59101 aagcgaggat aaatccaata atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg
    59161 gtaaaatcag cgtaaacggc gcaacgccga ggatagagcc ccaaatcaca tatttccgcc
    59221 caattttatc ccctacaggc ccgccgatca ccgtacctgc cgcaacggca aacaggaagg
    59281 caaacagatg aagctgagca ttctggatag ataatccgaa tttttgcatc agataaaagg
    59341 tgtaatagct gctgatgctc gccatataga aatatttcga gaaaatgagg attaacagaa
    59401 tgctgaccgc cagtaccact ttgttgcgcg gcagtggatt gataatcgtc gctttgggtt
    59461 ttcctttatt cattcggtgc tgtgccgagt accaacggct gatttgcgcc aacaccacga
    59521 tcgccagcag tgccgcaagc acaaaccagg caacgttgcc tttgccataa ggcgcgataa
    59581 tcaccgccgc cagcaagggt cccagggaac tgccaaagtt gccgccgacc tgaaagatag
    59641 attgcgccag gccatgccgc ccgccggaag ccatacgggc tacgcgagaa gattccggat
    59701 gaaagaccga tgaaccggta ccgaccagcg ccgccgccag cagaactgcg ccaaaactgc
    59761 ccgccagcgc aagcagcacc agaccactta aggtaaagca catgccaatt ggcaacgacc
    59821 acggcatcgg atatctatcg gtccagtagc cgaccactgg ttgcagtagc gaagaggcga
    59881 gctggaaggt gagggttatc atgccaatct gcataaatgt cagagaaaat tctgactgaa
    59941 gcagcggata aatcgccaga atcagcgatt ggatcatgtc gttcagcaga tgtgagaggc
    60001 tgatagcacc taaaatacca aacgatgttc gggccttggt cgttgacgca gccgcgcccg
    60061 ccacaggctg gggttgttca ctcattgcca taggaaagtc actttttcag ggttgcgatg
    60121 taaagaatga tcttatttgt gattattacc cgactaacat acctgtatgc gtcgcctgaa
    60181 ggaagtctca acgccgaata cagaatttct aatctggatg cagatttatc ttcaccggac
    60241 gcagacttgt ctatgatgtc gcgtcatact atttttcaac acgttgaaat caggtcaggg
    60301 agagaagtat gaaattattg cagcggggcg tggcgttagc gctgttaacc acatttacac
    60361 tggcgagtga aactgctctg gcgtatgagc aggataaaac ctacaaaatt acagttctgc
    60421 ataccaatga tcatcatggg catttttggc gcaatgaata tggcgaatat ggtctggcgg
    60481 cgcaaaaaac gctggtggat ggtatccgca aagaggttgc ggctgaaggc ggtagcgtgc
    60541 tgctactttc cggtggcgac attaacactg gcgtgcccga gtctgactta caggatgccg
    60601 aacctgattt tcgcggtatg aatctggtgg gctatgacgc gatggcgatc ggtaatcatg
    60661 aatttgataa tccgctcacc gtattacgcc agcaggaaaa gtgggccaag ttcccgttgc
    60721 tttccgcgaa tatctaccag aaaagtactg gcgagcgcct gtttaaaccg tgggcgctgt
    60781 ttaagcgtca ggatctgaaa attgccgtca ttgggctgac aaccgatgac acagcaaaaa
    60841 ttggtaaccc ggaatacttc actgatgtcg aatttcgtaa gcccgccgat gaagcgaagc
    60901 tggtgattca ggagctgcaa cagacagaaa agccagacat tattatcgcg gcgacccata
    60961 tggggcatta cgataatggt gagcacggct ctaacgcacc gggcgatgtg gagatggcac
    61021 gcgcgctgcc tgccggatcg ctggcgatga tcgtcggtgg tcactcgcaa gatccggtct
    61081 gcatggcggc agaaaacaaa aaacaggtcg attacgtgcc gggtacgcca tgcaagccag
    61141 atcaacaaaa cggcatctgg attgtgcagg cgcatgagtg gggcaaatac gtgggacggg
    61201 ctgattttga gtttcgtaat ggcgaaatga aaatggttaa ctaccagctg attccggtga
    61261 acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga gcgcgtgctt tacactcctg
    61321 aaatcgctga aaaccagcaa atgatctcgc tgttatcacc gttccagaac aaaggcaaag
    61381 cgcagctgga agtgaaaata ggcgaaacca atggtcgtct ggaaggcgat cgtgacaaag
    61441 tgcgttttgt acagaccaat atggggcggt tgattctggc agcccaaatg gatcgcactg
    61501 gtgccgactt tgcggtgatg agcggaggcg gaattcgtga ttctatcgaa gcaggcgata
    61561 tcagctataa aaacgtgctg aaagtgcagc cattcggcaa tgtggtggtg tatgccgaca
    61621 tgaccggtaa agaggtgatt gattacctga ccgccgtcgc gcagatgaag ccagattcag
    61681 gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa agacggcaaa ctgaacgacc
    61741 ttaaaatcaa aggcgaaccg gtcgatccgg cgaaaactta ccgtatggcg acattaaact
    61801 tcaatgccac cggcggtgat ggatatccgc gccttgataa caaaccgggc tatgtgaata
    61861 ccggctttat tgatgccgaa gtgctgaaag cgtatatcca gaaaagctcg ccgctggatg
    61921 tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta atccgaaagt gccggatgtt
    61981 tgcatccggc acaatgctta atcgcggcgg gcgatatcag caaatttggc atcgaggatc
    62041 tttgccagat cgcctgccgc cagttcgata tccagtccgc gcttgccgcc ggaaacataa
    62101 atagtggcaa attcttgtgc gggggcgtcg ataatcgttg gcagacgttt tttctgcccc
    62161 agtgggctaa tccccccaac caggtatccc gtcgaacgct gcgcgaccat cggatcggcc
    62221 atctcaactt tcttggcacc cagcgctttt gctacttttt taagatccag ttgacctgcg
    62281 accggcgtaa cggccacggc aaggtgtttc atatcaccgt tcactgccac cagcagcgtt
    62341 ttgtagacct gatccggatt caaacctaat tttttgacga cttcatcgcc aaaattggtt
    62401 tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa tcttgttttt ttcgagtaat
    62461 ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa aaaaacgaag caagcatacg
    62521 cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga gtattggcag gatggtgaga
    62581 ttgagcgaca atcgagttac accgtcgctt aaagtgacgg cataataata aaaaaatgaa
    62641 attcctcttt gacgggccaa tagcgatatt ggccattttt ttagcgcaac atttgcggca
    62701 aattcccttc tccatacagg tgtagtgcac cgaccgcgac cacatatcgc cccggcggca
    62761 tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg catcagcaca tcgtacagcg
    62821 actgactgaa cgtattgggc agcgttattt cattattttg cggcggcgca ttcagccacc
    62881 agctcatcat ttgttgcagc aaccgtgcgt tggtatgcca gtgggtcagc gtatcgtcca
    62941 gcagcgccag tcctttgtca gggagctgga gcaacatggc aatctggttt tcagcccctt
    63001 ccagttcaat cacgggttta tgttgttgct tcgccgcctg caatagctgg taatcgatac
    63061 cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc ctgaagaacc atcgcgattt
    63121 gccacagcgg ttgggtagaa aagagtgagg gagaaatgcc catctcctgg ctaatgtgct
    63181 gcagattttg gagttgctcc tcgctaatgc gctcttccag cgcctcgcag gcaggcaaat
    63241 tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac gatcagcgca tcggcgtttt
    63301 tgagcttttt gagcaaacgg gtgggcaggg gagccatatc gtggctaccc atatgaatac
    63361 tgccaatcag atgaaaatgg cgattgccgg ggagggtgat atcgatggca ggccaggtgt
    63421 aatgattacc gcgcagcgcg gcccaaagcg tttttacccg gtacaacaga tccatacgac
    63481 ctccctttgt gaaatatcat gctagcgcgc ggtgagggat ggcgcaaatg gagttgggga
    63541 cttgtttaat gtgtttgtat gattcagtat gttcttgcat cgctattcac agggaagcaa
    63601 cagttaaaaa ccatgaaaca ggcaacaaga aaaccgacga cacccggaga tattcttctc
    63661 tatgaatatc tggaaccgct cgatttgaaa atcaatgagt tagcagagtt gctgcatgtt
    63721 catcgtaata gcgtcagtgc actgatcaat aacaatcgta aactcaccac tgagatggca
    63781 ttccgtctgg cgaaagtttt tgataccaca gtcgatttta ggctaaacct ccaggcggca
    63841 gttgatcttt gggaagttga aaacaacatg cgcacccagg aagaattggg acggattgaa
    63901 acagtggctg aatatttggc acgccgtgaa gagcgtgcaa aaaaggtcgc gtaaggccaa
    63961 aacatcgctt catctcgtag gccggataag gcgtttacgc cgcatccggc agtcatgcgt
    64021 cgatgccaaa tgcgccgccc taaagcagcg catccgcaat gacgtactta ttccttcggt
    64081 ttaaaccgca gcaaccggtt ggcgttactc actacggtaa tcgacgagag cgccattgcc
    64141 gctccggcaa ctaccgggtt aagcagtgtt ccagtaaacg gccacaaaat accggcggcg
    64201 accggaatac cgatgctgtt gtagataaag gcaccgagca ggttctgctt catgttgcgc
    64261 agcgttgcgc gggaaatcgc gagcgcatcc gcaacgccca tcaggctatg gcgcatcagg
    64321 gtaatcgccg cggtttcaat ggcaacatca ctgccgccac ccatcgcaat gccgacatcc
    64381 gcctgggcca gcgctggcgc gtcgttaatg ccgtcgccca ccattgccac ctgacgtcct
    64441 tcactttgca gacgtttgat cgcttcggct ttaccgtccg gcagcactcc ggcaatcact
    64501 tcatcaattc cagcttcttt ggcgatcgca ttggcggtgg ttgggttatc cccggtcaac
    64561 atcaccagac gatatcccgc tttatgcagg cgttgcagcg ccgccacgct atcactacgc
    64621 aacggatcgc gtacagccag cagggctact gctttcccgt caatcgccag cagcacaggc
    64681 gttgctcctt gcgatgcctg agcagtaatc tccgcttcga tagctttggt accaacctgt
    64741 tgctcattta acagcgcctg attgcccagc aataacgcat gaccttcagc ttcaccgctc
    64801 acgcccagcc cgcgcaatgt gcggaaaccg ttgacctgcg gcagctgcat atcacctgct
    64861 ttatcgagga tcgcccgtgc cagcggatgg ctggaacctt gctccagtgc cgccgccaga
    64921 cgcaatgcct gcgcttcatc aacatcagca aacgttttca ctgcgacaac ctgcggcttc
    64981 ccttcggtca gcgtcccggt tttatcgaac acgacggtat cgagtgtact ggcgcgttgc
    65041 agcgcgtcgg cgtcccgcac cagcacgcca aactcagccg cccgcccgac gccggaaata
    65101 atcgacatcg gcgtcgccag ccccagcgca cacggacagg cgataatcag taccgtggtg
    65161 gcaatcacca gggtataaac aatttgcggt gccggaccaa agaagtacca gattgccgca
    65221 ctgaccagcg caatcaccac caccacaggt acaaatacgg ctgagatttt atccgccagc
    65281 tgaccgattt ctggcttgct gctctgggcc tggcgcacca tgcgaatgat ccgtgacagc
    65341 gtggtatggc tgccaaccgc actggcacga aacagcacgc taccgtcctg taccactgtc
    65401 ccggcatgga cgctttcgcc ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc
    65461 gcttcatcca gccatgcttc gccctgggta atctcgccat ctaccggcac gcgatcgccg
    65521 gtcgtcaggc gcagcaacat acctggctgc acttccgcca gaggcacgct tttttcacct
    65581 tcgtcagtaa ccaggcgcgc cgtcggtggg gttaaatcga gtaatttctc cagcgcctta
    65641 gaagaacgct gacgtgcgcg tgcttccagc atatggccga gattgatcag accgataatc
    65701 atcgcgctgg cttcgtaata aagatgtcgc gcttccatcg ggaaccactg cggccacagg
    65761 ttgacgctca tcgaatagag ccacgccacg ccagtaccca gcgccaccag cgtatccatc
    65821 gtcgctgcac cgttcagcag gcttttccat gcactgcggt aaaaatggcc gccggcgaaa
    65881 accatcaccg ccagggttat caggccgata accaaccaca ggctgcggtt gtcagcggtg
    65941 accatcatgt tatcgccaat catcccccag accatcaccg ggatacccac cgccagtgcg
    66001 acaattgcct gccagcggaa gcgcttcatc gtagcaacgg cggtttcctg ctggcgctca
    66061 cggcgtttcg cgtcatcttc aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc
    66121 actaaatctt gtggggaggc actgcccatc accagcgcag tacgctccgc caggtttacc
    66181 cgtgcctgag tgacgcccgg tacgctttgc agcgcatttt gtacgcgggt gacacagctg
    66241 gcgcagctca tgccgctcag cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc
    66301 ggaagcgcct cagaaaccgc tgtcagtgct tccgacggga ttgatgactc cgccagcggt
    66361 ttagcctttg ggtggcttac agatgcgtca taacccgctt gtttgatggt ttcgatcagc
    66421 tgttctgcac tggcagtccc ggtaacgtgc gcttcagtga tagacacatc cgcttgttcc
    66481 acatccgggc gctgttcgag actttctttc acgcgtttaa cgcagtgacc gcaggacagg
    66541 ccgtccaggg tcaggtcgat agtttgtgac ataaaacact cctttaagac agttttgact
    66601 ggctgtgata aaggttaaac cttccagcaa ggggaaggtc aagaaattaa taaaccaggc
    66661 gagtaaaagt ccgtaaagat taaaaaattg gctcaatttg catcaggatt agacatttat
    66721 ctctttgttt tcctgaagtt aagttgcggg tgctaagtta aatcaagatg cctgaaaatc
    66781 ggcaccgggg tgaggaatta cctcccgcat ctataaaaag gagttaacaa aagatgttag
    66841 atgcaaacaa attacagcag gcagtggatc aggcttacac ccaatttcac tcacttaacg
    66901 gcggacaaaa tgccgactac attccctttc tggcgaatgt acctagtcaa ctggcagcag
    66961 tggctattgt gacctgcgat ggcaacatct atcgcgcggg tgatagtgat taccgctttg
    67021 cgctggaatc catctcgaaa gtctgtacgt tagcccttgc gttagaagat gtcggcccgc
    67081 aggcggtaca ggacaaaatt ggcgctgacc cgaccggatt gccctttaac tcagttatcg
    67141 ccttagagtt gcatggcggc aaaccactgt cgccactggt aaatgctggc gctattgcca
    67201 ccaccagcct gattaacgct gaaaatattg aacaacgctg gcagcgaatt ttacatatcc
    67261 aacagcaact ggctggtgag caggtagcgc tctctgatga agtcaaccag tcggaacaaa
    67321 caaccaactt ccataaccgg gccattgcct ggctgctgta ctccgccgga tatctctatt
    67381 gtgatgcaat ggaagcctgt gacgtgtaca cccgtcagtg ctccacgctc atcaatactg
    67441 ttgaactggc aacgcttggc gcgacgctgg cggcgggtgg tgtgaatccg ctgacgcata
    67501 aacgcgttct tcaggccgac aacgtgccgt acattctggc cgaaatgatg atggaagggc
    67561 tgtatggtcg ctccggtgac tgggcgtatc gcgttggttt accgggcaaa agcggtgtag
    67621 gtggcggtat tctggcggtc gtccctggcg tgatgggaat tgccgcgttc tcaccaccgc
    67681 tggacgaaga aggcaacagt gttcgcggtc aaaaaatggt ggcatcggtc gctaagcaac
    67741 tcggctataa cgtgtttaag ggctgatcat gatgaacacg gaaggtaata acggtaacaa
    67801 acctctcggt ctatggaacg tcgtttccat cg
//
LOCUS       DAZFVO020000003        67379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFVO020000003 DAZFVO020000000
VERSION     DAZFVO020000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 67379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.003"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            79..697
                     /locus_tag="ACHKNB_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /pseudo
     CDS             79..697
                     /locus_tag="ACHKNB_RS00655"
                     /old_locus_tag="pgaptmp_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            800..1303
                     /gene="yfbM"
                     /locus_tag="ACHKNB_RS00660"
                     /old_locus_tag="pgaptmp_000132"
     CDS             800..1303
                     /gene="yfbM"
                     /locus_tag="ACHKNB_RS00660"
                     /old_locus_tag="pgaptmp_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HGU4193718.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1370..2827)
                     /gene="nuoN"
                     /locus_tag="ACHKNB_RS00665"
                     /old_locus_tag="pgaptmp_000133"
     CDS             complement(1370..2827)
                     /gene="nuoN"
                     /locus_tag="ACHKNB_RS00665"
                     /old_locus_tag="pgaptmp_000133"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HGU4193719.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRVLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2834..4363)
                     /gene="nuoM"
                     /locus_tag="ACHKNB_RS00670"
                     /old_locus_tag="pgaptmp_000134"
     CDS             complement(2834..4363)
                     /gene="nuoM"
                     /locus_tag="ACHKNB_RS00670"
                     /old_locus_tag="pgaptmp_000134"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HGU4193720.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4527..6368)
                     /gene="nuoL"
                     /locus_tag="ACHKNB_RS00675"
                     /old_locus_tag="pgaptmp_000135"
     CDS             complement(4527..6368)
                     /gene="nuoL"
                     /locus_tag="ACHKNB_RS00675"
                     /old_locus_tag="pgaptmp_000135"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HGU4193721.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6365..6667)
                     /gene="nuoK"
                     /locus_tag="ACHKNB_RS00680"
                     /old_locus_tag="pgaptmp_000136"
     CDS             complement(6365..6667)
                     /gene="nuoK"
                     /locus_tag="ACHKNB_RS00680"
                     /old_locus_tag="pgaptmp_000136"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HGU4193722.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6664..7218)
                     /gene="nuoJ"
                     /locus_tag="ACHKNB_RS00685"
                     /old_locus_tag="pgaptmp_000137"
     CDS             complement(6664..7218)
                     /gene="nuoJ"
                     /locus_tag="ACHKNB_RS00685"
                     /old_locus_tag="pgaptmp_000137"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HGU4193723.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7230..7772)
                     /gene="nuoI"
                     /locus_tag="ACHKNB_RS00690"
                     /old_locus_tag="pgaptmp_000138"
     CDS             complement(7230..7772)
                     /gene="nuoI"
                     /locus_tag="ACHKNB_RS00690"
                     /old_locus_tag="pgaptmp_000138"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HGU4193724.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7787..8764)
                     /gene="nuoH"
                     /locus_tag="ACHKNB_RS00695"
                     /old_locus_tag="pgaptmp_000139"
     CDS             complement(7787..8764)
                     /gene="nuoH"
                     /locus_tag="ACHKNB_RS00695"
                     /old_locus_tag="pgaptmp_000139"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HGU4193725.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8761..11487)
                     /gene="nuoG"
                     /locus_tag="ACHKNB_RS00700"
                     /old_locus_tag="pgaptmp_000140"
     CDS             complement(8761..11487)
                     /gene="nuoG"
                     /locus_tag="ACHKNB_RS00700"
                     /old_locus_tag="pgaptmp_000140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HGU4193726.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11540..12877)
                     /gene="nuoF"
                     /locus_tag="ACHKNB_RS00705"
                     /old_locus_tag="pgaptmp_000141"
     CDS             complement(11540..12877)
                     /gene="nuoF"
                     /locus_tag="ACHKNB_RS00705"
                     /old_locus_tag="pgaptmp_000141"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HGU4193727.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLHSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12874..13374)
                     /gene="nuoE"
                     /locus_tag="ACHKNB_RS00710"
                     /old_locus_tag="pgaptmp_000142"
     CDS             complement(12874..13374)
                     /gene="nuoE"
                     /locus_tag="ACHKNB_RS00710"
                     /old_locus_tag="pgaptmp_000142"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HGU4193728.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13377..15179)
                     /gene="nuoC"
                     /locus_tag="ACHKNB_RS00715"
                     /old_locus_tag="pgaptmp_000143"
     CDS             complement(13377..15179)
                     /gene="nuoC"
                     /locus_tag="ACHKNB_RS00715"
                     /old_locus_tag="pgaptmp_000143"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HGU4193729.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15273..15935)
                     /gene="nuoB"
                     /locus_tag="ACHKNB_RS00720"
                     /old_locus_tag="pgaptmp_000144"
     CDS             complement(15273..15935)
                     /gene="nuoB"
                     /locus_tag="ACHKNB_RS00720"
                     /old_locus_tag="pgaptmp_000144"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HGU4193730.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15951..16394)
                     /gene="nuoA"
                     /locus_tag="ACHKNB_RS00725"
                     /old_locus_tag="pgaptmp_000145"
     CDS             complement(15951..16394)
                     /gene="nuoA"
                     /locus_tag="ACHKNB_RS00725"
                     /old_locus_tag="pgaptmp_000145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HGU4193731.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17025..17963)
                     /gene="lrhA"
                     /locus_tag="ACHKNB_RS00730"
                     /old_locus_tag="pgaptmp_000146"
     CDS             complement(17025..17963)
                     /gene="lrhA"
                     /locus_tag="ACHKNB_RS00730"
                     /old_locus_tag="pgaptmp_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HGU4193732.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18883..20100
                     /gene="alaA"
                     /locus_tag="ACHKNB_RS00735"
                     /old_locus_tag="pgaptmp_000147"
     CDS             18883..20100
                     /gene="alaA"
                     /locus_tag="ACHKNB_RS00735"
                     /old_locus_tag="pgaptmp_000147"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HGU4193733.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20184..20783
                     /gene="yfbR"
                     /locus_tag="ACHKNB_RS00740"
                     /old_locus_tag="pgaptmp_000148"
     CDS             20184..20783
                     /gene="yfbR"
                     /locus_tag="ACHKNB_RS00740"
                     /old_locus_tag="pgaptmp_000148"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4193734.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20842..22674)
                     /gene="yfbS"
                     /locus_tag="ACHKNB_RS00745"
                     /old_locus_tag="pgaptmp_000149"
     CDS             complement(20842..22674)
                     /gene="yfbS"
                     /locus_tag="ACHKNB_RS00745"
                     /old_locus_tag="pgaptmp_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4193735.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLVDEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22761..23411)
                     /gene="hxpA"
                     /locus_tag="ACHKNB_RS00750"
                     /old_locus_tag="pgaptmp_000150"
     CDS             complement(22761..23411)
                     /gene="hxpA"
                     /locus_tag="ACHKNB_RS00750"
                     /old_locus_tag="pgaptmp_000150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HGU4193736.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23422..23916)
                     /gene="yfbU"
                     /locus_tag="ACHKNB_RS00755"
                     /old_locus_tag="pgaptmp_000151"
     CDS             complement(23422..23916)
                     /gene="yfbU"
                     /locus_tag="ACHKNB_RS00755"
                     /old_locus_tag="pgaptmp_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HGU4193737.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23999..24454)
                     /gene="yfbV"
                     /locus_tag="ACHKNB_RS00760"
                     /old_locus_tag="pgaptmp_000152"
     CDS             complement(23999..24454)
                     /gene="yfbV"
                     /locus_tag="ACHKNB_RS00760"
                     /old_locus_tag="pgaptmp_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HGU4193738.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24792..25994
                     /gene="ackA"
                     /locus_tag="ACHKNB_RS00765"
                     /old_locus_tag="pgaptmp_000153"
     CDS             24792..25994
                     /gene="ackA"
                     /locus_tag="ACHKNB_RS00765"
                     /old_locus_tag="pgaptmp_000153"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGU4193739.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26069..28213
                     /gene="pta"
                     /locus_tag="ACHKNB_RS00770"
                     /old_locus_tag="pgaptmp_000154"
     CDS             26069..28213
                     /gene="pta"
                     /locus_tag="ACHKNB_RS00770"
                     /old_locus_tag="pgaptmp_000154"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4193740.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28403..29923
                     /gene="yfcC"
                     /locus_tag="ACHKNB_RS00775"
                     /old_locus_tag="pgaptmp_000155"
     CDS             28403..29923
                     /gene="yfcC"
                     /locus_tag="ACHKNB_RS00775"
                     /old_locus_tag="pgaptmp_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HGU4193741.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSELRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29956..30498)
                     /gene="yfcD"
                     /locus_tag="ACHKNB_RS00780"
                     /old_locus_tag="pgaptmp_000156"
     CDS             complement(29956..30498)
                     /gene="yfcD"
                     /locus_tag="ACHKNB_RS00780"
                     /old_locus_tag="pgaptmp_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HGU4193742.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30556..31107)
                     /gene="yfcE"
                     /locus_tag="ACHKNB_RS00785"
                     /old_locus_tag="pgaptmp_000157"
     CDS             complement(30556..31107)
                     /gene="yfcE"
                     /locus_tag="ACHKNB_RS00785"
                     /old_locus_tag="pgaptmp_000157"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HGU4193743.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31163..31807)
                     /gene="yfcF"
                     /locus_tag="ACHKNB_RS00790"
                     /old_locus_tag="pgaptmp_000158"
     CDS             complement(31163..31807)
                     /gene="yfcF"
                     /locus_tag="ACHKNB_RS00790"
                     /old_locus_tag="pgaptmp_000158"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HGU4193744.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31943..32590
                     /gene="yfcG"
                     /locus_tag="ACHKNB_RS00795"
                     /old_locus_tag="pgaptmp_000159"
     CDS             31943..32590
                     /gene="yfcG"
                     /locus_tag="ACHKNB_RS00795"
                     /old_locus_tag="pgaptmp_000159"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HGU4193745.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32647..33009
                     /gene="folX"
                     /locus_tag="ACHKNB_RS00800"
                     /old_locus_tag="pgaptmp_000160"
     CDS             32647..33009
                     /gene="folX"
                     /locus_tag="ACHKNB_RS00800"
                     /old_locus_tag="pgaptmp_000160"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HGU4193746.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33030..33923
                     /gene="yfcH"
                     /locus_tag="ACHKNB_RS00805"
                     /old_locus_tag="pgaptmp_000161"
     CDS             33030..33923
                     /gene="yfcH"
                     /locus_tag="ACHKNB_RS00805"
                     /old_locus_tag="pgaptmp_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HGU4193747.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33971..34861)
                     /gene="rpnB"
                     /locus_tag="ACHKNB_RS00810"
                     /old_locus_tag="pgaptmp_000162"
     CDS             complement(33971..34861)
                     /gene="rpnB"
                     /locus_tag="ACHKNB_RS00810"
                     /old_locus_tag="pgaptmp_000162"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HGU4193748.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVLMVTRLSPDDLIAQSH"
     gene            complement(35058..35831)
                     /gene="hisP"
                     /locus_tag="ACHKNB_RS00815"
                     /old_locus_tag="pgaptmp_000163"
     CDS             complement(35058..35831)
                     /gene="hisP"
                     /locus_tag="ACHKNB_RS00815"
                     /old_locus_tag="pgaptmp_000163"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HGU4193749.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35839..36555)
                     /gene="hisM"
                     /locus_tag="ACHKNB_RS00820"
                     /old_locus_tag="pgaptmp_000164"
     CDS             complement(35839..36555)
                     /gene="hisM"
                     /locus_tag="ACHKNB_RS00820"
                     /old_locus_tag="pgaptmp_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4193750.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36552..37238)
                     /gene="hisQ"
                     /locus_tag="ACHKNB_RS00825"
                     /old_locus_tag="pgaptmp_000165"
     CDS             complement(36552..37238)
                     /gene="hisQ"
                     /locus_tag="ACHKNB_RS00825"
                     /old_locus_tag="pgaptmp_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HGU4193751.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37328..38110)
                     /gene="hisJ"
                     /locus_tag="ACHKNB_RS00830"
                     /old_locus_tag="pgaptmp_000166"
     CDS             complement(37328..38110)
                     /gene="hisJ"
                     /locus_tag="ACHKNB_RS00830"
                     /old_locus_tag="pgaptmp_000166"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HGU4193752.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38331..39113)
                     /gene="argT"
                     /locus_tag="ACHKNB_RS00835"
                     /old_locus_tag="pgaptmp_000167"
     CDS             complement(38331..39113)
                     /gene="argT"
                     /locus_tag="ACHKNB_RS00835"
                     /old_locus_tag="pgaptmp_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HGU4193753.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39379..39948)
                     /gene="ubiX"
                     /locus_tag="ACHKNB_RS00840"
                     /old_locus_tag="pgaptmp_000168"
     CDS             complement(39379..39948)
                     /gene="ubiX"
                     /locus_tag="ACHKNB_RS00840"
                     /old_locus_tag="pgaptmp_000168"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HGU4193754.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40043..41560)
                     /gene="purF"
                     /locus_tag="ACHKNB_RS00845"
                     /old_locus_tag="pgaptmp_000169"
     CDS             complement(40043..41560)
                     /gene="purF"
                     /locus_tag="ACHKNB_RS00845"
                     /old_locus_tag="pgaptmp_000169"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGU4193755.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41597..42085)
                     /gene="cvpA"
                     /locus_tag="ACHKNB_RS00850"
                     /old_locus_tag="pgaptmp_000170"
     CDS             complement(41597..42085)
                     /gene="cvpA"
                     /locus_tag="ACHKNB_RS00850"
                     /old_locus_tag="pgaptmp_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HGU4193756.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42344..43006)
                     /gene="dedD"
                     /locus_tag="ACHKNB_RS00855"
                     /old_locus_tag="pgaptmp_000171"
     CDS             complement(42344..43006)
                     /gene="dedD"
                     /locus_tag="ACHKNB_RS00855"
                     /old_locus_tag="pgaptmp_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HGU4193757.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42996..44264)
                     /gene="folC"
                     /locus_tag="ACHKNB_RS00860"
                     /old_locus_tag="pgaptmp_000172"
     CDS             complement(42996..44264)
                     /gene="folC"
                     /locus_tag="ACHKNB_RS00860"
                     /old_locus_tag="pgaptmp_000172"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HGU4193758.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44334..45248)
                     /gene="accD"
                     /locus_tag="ACHKNB_RS00865"
                     /old_locus_tag="pgaptmp_000173"
     CDS             complement(44334..45248)
                     /gene="accD"
                     /locus_tag="ACHKNB_RS00865"
                     /old_locus_tag="pgaptmp_000173"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGU4193759.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45404..46063)
                     /gene="dedA"
                     /locus_tag="ACHKNB_RS00870"
                     /old_locus_tag="pgaptmp_000174"
     CDS             complement(45404..46063)
                     /gene="dedA"
                     /locus_tag="ACHKNB_RS00870"
                     /old_locus_tag="pgaptmp_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4193760.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46146..46958)
                     /gene="truA"
                     /locus_tag="ACHKNB_RS00875"
                     /old_locus_tag="pgaptmp_000175"
     CDS             complement(46146..46958)
                     /gene="truA"
                     /locus_tag="ACHKNB_RS00875"
                     /old_locus_tag="pgaptmp_000175"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGU4193761.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46958..47971)
                     /gene="usg"
                     /locus_tag="ACHKNB_RS00880"
                     /old_locus_tag="pgaptmp_000176"
     CDS             complement(46958..47971)
                     /gene="usg"
                     /locus_tag="ACHKNB_RS00880"
                     /old_locus_tag="pgaptmp_000176"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4193762.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48037..49173)
                     /gene="pdxB"
                     /locus_tag="ACHKNB_RS00885"
                     /old_locus_tag="pgaptmp_000177"
     CDS             complement(48037..49173)
                     /gene="pdxB"
                     /locus_tag="ACHKNB_RS00885"
                     /old_locus_tag="pgaptmp_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HGU4193763.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGYVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49272..50267
                     /gene="flk"
                     /locus_tag="ACHKNB_RS00890"
                     /old_locus_tag="pgaptmp_000178"
     CDS             49272..50267
                     /gene="flk"
                     /locus_tag="ACHKNB_RS00890"
                     /old_locus_tag="pgaptmp_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HGU4193764.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50264..51442)
                     /gene="yfcJ"
                     /locus_tag="ACHKNB_RS00895"
                     /old_locus_tag="pgaptmp_000179"
     CDS             complement(50264..51442)
                     /gene="yfcJ"
                     /locus_tag="ACHKNB_RS00895"
                     /old_locus_tag="pgaptmp_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4193765.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51707..52927)
                     /gene="fabB"
                     /locus_tag="ACHKNB_RS00900"
                     /old_locus_tag="pgaptmp_000180"
     CDS             complement(51707..52927)
                     /gene="fabB"
                     /locus_tag="ACHKNB_RS00900"
                     /old_locus_tag="pgaptmp_000180"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HGU4193766.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53086..55092
                     /gene="mnmC"
                     /locus_tag="ACHKNB_RS00905"
                     /old_locus_tag="pgaptmp_000181"
     CDS             53086..55092
                     /gene="mnmC"
                     /locus_tag="ACHKNB_RS00905"
                     /old_locus_tag="pgaptmp_000181"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HGU4193767.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55213..55491)
                     /gene="yfcL"
                     /locus_tag="ACHKNB_RS00910"
                     /old_locus_tag="pgaptmp_000182"
     CDS             complement(55213..55491)
                     /gene="yfcL"
                     /locus_tag="ACHKNB_RS00910"
                     /old_locus_tag="pgaptmp_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HGU4193768.1"
                     /translation="MIAEFESRILALIDDMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55525..56073)
                     /gene="epmC"
                     /locus_tag="ACHKNB_RS00915"
                     /old_locus_tag="pgaptmp_000183"
     CDS             complement(55525..56073)
                     /gene="epmC"
                     /locus_tag="ACHKNB_RS00915"
                     /old_locus_tag="pgaptmp_000183"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HGU4193769.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56073..56882)
                     /gene="yfcA"
                     /locus_tag="ACHKNB_RS00920"
                     /old_locus_tag="pgaptmp_000184"
     CDS             complement(56073..56882)
                     /gene="yfcA"
                     /locus_tag="ACHKNB_RS00920"
                     /old_locus_tag="pgaptmp_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGU4193770.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(56882..57706)
                     /gene="mepA"
                     /locus_tag="ACHKNB_RS00925"
                     /old_locus_tag="pgaptmp_000185"
     CDS             complement(56882..57706)
                     /gene="mepA"
                     /locus_tag="ACHKNB_RS00925"
                     /old_locus_tag="pgaptmp_000185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HGU4193771.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57710..58795)
                     /gene="aroC"
                     /locus_tag="ACHKNB_RS00930"
                     /old_locus_tag="pgaptmp_000186"
     CDS             complement(57710..58795)
                     /gene="aroC"
                     /locus_tag="ACHKNB_RS00930"
                     /old_locus_tag="pgaptmp_000186"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGU4193772.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58830..59762)
                     /gene="prmB"
                     /locus_tag="ACHKNB_RS00935"
                     /old_locus_tag="pgaptmp_000187"
     CDS             complement(58830..59762)
                     /gene="prmB"
                     /locus_tag="ACHKNB_RS00935"
                     /old_locus_tag="pgaptmp_000187"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4193773.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59928..60479
                     /gene="smrB"
                     /locus_tag="ACHKNB_RS00940"
                     /old_locus_tag="pgaptmp_000188"
     CDS             59928..60479
                     /gene="smrB"
                     /locus_tag="ACHKNB_RS00940"
                     /old_locus_tag="pgaptmp_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HGU4193774.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60552..61406)
                     /locus_tag="ACHKNB_RS00945"
                     /old_locus_tag="pgaptmp_000189"
     CDS             complement(60552..61406)
                     /locus_tag="ACHKNB_RS00945"
                     /old_locus_tag="pgaptmp_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HGU4193775.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(61408..61947)
                     /locus_tag="ACHKNB_RS00950"
                     /old_locus_tag="pgaptmp_000190"
     CDS             complement(61408..61947)
                     /locus_tag="ACHKNB_RS00950"
                     /old_locus_tag="pgaptmp_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4193776.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(61944..62432)
                     /locus_tag="ACHKNB_RS00955"
                     /old_locus_tag="pgaptmp_000191"
     CDS             complement(61944..62432)
                     /locus_tag="ACHKNB_RS00955"
                     /old_locus_tag="pgaptmp_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4193777.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(62429..62938)
                     /locus_tag="ACHKNB_RS00960"
                     /old_locus_tag="pgaptmp_000192"
     CDS             complement(62429..62938)
                     /locus_tag="ACHKNB_RS00960"
                     /old_locus_tag="pgaptmp_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4193778.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(62954..63706)
                     /locus_tag="ACHKNB_RS00965"
                     /old_locus_tag="pgaptmp_000193"
     CDS             complement(62954..63706)
                     /locus_tag="ACHKNB_RS00965"
                     /old_locus_tag="pgaptmp_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4193779.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(63726..66371)
                     /gene="yfcU"
                     /locus_tag="ACHKNB_RS00970"
                     /old_locus_tag="pgaptmp_000194"
     CDS             complement(63726..66371)
                     /gene="yfcU"
                     /locus_tag="ACHKNB_RS00970"
                     /old_locus_tag="pgaptmp_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HGU4193780.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(66453..67016)
                     /gene="yfcV"
                     /locus_tag="ACHKNB_RS00975"
                     /old_locus_tag="pgaptmp_000195"
     CDS             complement(66453..67016)
                     /gene="yfcV"
                     /locus_tag="ACHKNB_RS00975"
                     /old_locus_tag="pgaptmp_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4193781.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16645 a        18306 c        16920 g        15508 t
ORIGIN      
        1 gctcataact cattataaat aatgataaaa taacgccttt tattgataat ttacatatga
       61 aaaaaatttc cttttgttat attatttctg ttttcactcc ctctgattat tttttaccaa
      121 ccctgggtca ctgcattatc gccaatgccc cgacacgtaa gtcctgagca attagaaaaa
      181 acagttcgtt atcttacaca aactgtgcat ccacgtaatg ccggcaatat tgataatctg
      241 aataggtccg tagagtatat aaaagaggtc tttgtcagta gcggtgccag agttaccgcg
      301 cagggcgtcc ccgattggct attacgactc tgcgcctgga agccagaatt acccttaccc
      361 ggcaatgtcc tggctttatc ccgatcgggg agactttatt gccgtagtcg gcagaatgca
      421 ggatatcaac gccgttcgtc aggtaaaatc ggcattgttg tcatcccggg atttatctgt
      481 ttattctatg aatgccccag ggtttattcc cggtattgat ttctctgatc accttaataa
      541 ttggcaacac gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca
      601 ataccacttg cccggtgata ccgcagacag attgaattat cagaaaatgg ctcaggtggt
      661 ggatggtgtt ataaacttgt tatacaacag taaataataa aaatttctcg aggcgggtcc
      721 agaaaaagcg acatgcacat tttattttcc ctcgtctgtt ttaagatgcg aaacatgaaa
      781 ttcaatggag gaaaaataaa tgggaatgat tggctatttt gcagaaattg actcagaaaa
      841 gattaaccaa ctgcttgagt ctactgaaaa acccttaatg gacaatattc atgacacgct
      901 ttcaggatta cgccgactgg atattgacaa acgctgggat tttttacact ttgggctgac
      961 aggcacctct gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg tgcttggtga
     1021 acacagcctc gaagatggca tagatggctt cctgggattg acctggaatc aggagttagc
     1081 cgcgacaatc gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac agttttccat
     1141 taagcgactc aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg
     1201 gcaactcttt gcctcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat
     1261 gctgcgacag gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa
     1321 aaaaaaccgc cgaacatgtc ggcggctttc tgacttacaa actgatagct tacatcagcg
     1381 gcattgctaa ccgcacaatg ctaatcagcg gttgtggcca tacacccagc accagtacca
     1441 acagtgcaga aatcagcacc acgataccgc ccgcgctgta ctgccagttt gacggtgcat
     1501 cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt
     1561 agaggccgat tgccgaaccg acaaccacag cacccaccag ccaccacaag tgtgcctgga
     1621 caccgaccgc cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca
     1681 gagacagcat catcaccgtc atcaccgccg cgaggatcgg gcgatgccag aacagaccgc
     1741 ggtaagagaa cagggaatca gcatccgggc cacggtacgg gctggacatc aggctgacca
     1801 cgccgaacgc gccgaggctg ctgaacagat aaccggccag gtaaactcct accgcttcca
     1861 tcgacatctc gcccgtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga
     1921 tagatgagta accgagcaca cgtttgatat tggtctggct cagcgccatc aggttaccga
     1981 agatgataga ggcaaaggcg ataatcgcca gcactacccg aatcgcttcg ctgtcaccca
     2041 ccggtgcgta gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg
     2101 ccaggaaagt ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga
     2161 acggcaccag agagagtttg aagccaaggc caacaatcat caggccgaaa cctgccagca
     2221 acagcggctc gttgagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt
     2281 cgccagactg cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag
     2341 aaaggatagt gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga
     2401 ccaggccaaa cagcggcaaa gagatcagtt cgataccgag gaacagagac gccagatggt
     2461 tggcattcgc cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat
     2521 ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg
     2581 ccaacaatac cagcccggtg taaagcatgg cgaaaccatc aacgcgcatc agcggcgtaa
     2641 cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa
     2701 taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa
     2761 ccaccaccgt caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta
     2821 ttgtcatggc gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat
     2881 tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca
     2941 gcagcaccac cagcaacaag atcataaaca gctcacgcag cgacatccct ggcagttcct
     3001 ggctggcaat ctggcttttc gctttaccga agtaagcgcg atgcaacatc gccagcgaat
     3061 aaacagatgc aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga
     3121 agctgccgaa cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg
     3181 ttgccaccgc aaagaacagc gacagtgctg gcagccattt catcttgctc cacagaccgc
     3241 ccatcatgcg catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca
     3301 gacccgccgc cgacaaaccg tgcgcaatca tctggattac cgcgccctgg taggccaact
     3361 ggctgccggt gtagatagca atcagcacga agcccatgtg ggaaaccgag gtgtaggcga
     3421 tcagacgttt gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa
     3481 cacccagcca catagcaatt ggcgcgaact ctgccgacgc gttcgggaac agcggcaggg
     3541 agaaacgcag caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg tcaacggaac
     3601 cggcggtcgg agcctgggag tgcgcatccg gcagccagcc atgcagcgga accaccggca
     3661 ttttgactgc gaaggcgatg aagaagccca gcattaacag gtattccaca ccactggaca
     3721 ttggcgtatt cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa
     3781 caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc actcgcctgg gtgtaaatga
     3841 agaacttggt tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg
     3901 cgatcaggaa gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt
     3961 cgatggcaag gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga
     4021 agccctgata tttttcgatc tctttccacg aacatagtac cgccagcaca ccgagcagac
     4081 cggtcagcac gaccatcagc agcgacagac cgtcaatggc gagatgaata gagataccaa
     4141 aacgcgggat ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg
     4201 tcagtgaata accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg
     4261 tgatcagcgc gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga
     4321 agccgccaat aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt
     4381 gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg
     4441 actgggggta gtgtcggatg cgccgcgaag gcagcttatc cgacctacgt tcttatatgc
     4501 ccaggggcaa tcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg
     4561 accgcaccga tgctcatgga tgccacatac cagcgcagat aaccgttctc gcttaacagc
     4621 agacctttac ctgcaaagcg ggaaagaacc gccgggatgt tcatcattga gttcagcgga
     4681 tcgcgtttca gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcgtacagc
     4741 cagtcaaagc cccaggcgtt gtaccaccag gtactcagca ggcggcccgg cgcactgttg
     4801 gcgatggagg tcaccagagt acgtttaccc agccacagcc aggctgccag cagaatgccg
     4861 accaccgcga ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc
     4921 gtcgtttgcg gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa
     4981 aggatcagca gcacaatcag cggcaggctg tgagttaccc ctttcacggc gtgagcgtga
     5041 atttgttctt ttccgtggaa gacgatgaaa atcatacgga aggtgtagag cgaggtcata
     5101 aacgcaccga ccagacctgc caccatcaga ttgatatgac cattcgccat cgcacccgcg
     5161 aggatctcat ccttactgaa gaagcccgca gtgaccagcg gtagtgccga cagtgctgcg
     5221 ccgcccacca ggaagcagag ataaaccagc ggaatagatt tacgcagacc gcccatcttg
     5281 aagatgttct gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc
     5341 gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg catcccatgc ctgcacgcca
     5401 agcgcgagga acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg
     5461 tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc gacaataccc
     5521 accagatgca gaacttccgg cgtcatcagg aacaggccgt gggtacgggc gatcaggtag
     5581 acacccgcgg ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc
     5641 atcgcgtcag caagccatgt ctgcaacggc aactgcgcag atttaccgac cgcaccgccc
     5701 agcagcatca gcgtcgccca catcagcatg ttattgccgt cagcaaagtg cgctggtgcc
     5761 agttccacca tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagtgcg
     5821 aaagcgagga aaacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcgcca
     5881 ttcttcggat cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc
     5941 cagccgaggt acatcagcag caggttatcg gcgagaacca gaactaccat gctggcgatg
     6001 aacaggttgg tgtaggcgaa gaagcgagag tagccctctt caccgcgcat gtaccaggag
     6061 gcgtacatgt gaataaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg
     6121 ccgtccagca ccaggttaaa accgatgtta aagtcgccta ccgacatcca cgtccacagc
     6181 ggctggctgt atgcctgctc gccgttagcg aagaaatcaa cgccgataaa ggcggttacc
     6241 agcgccgcca ggcccacaga gccaacgccg acgatcgccg aaacgttttc agaccagcgc
     6301 ccacgggaga acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc
     6361 atgttcatcc gcgcatctca cttactgaat cgatgttcag gttctggcga cgacggtgaa
     6421 gttgcagcag cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg atggcgagaa
     6481 tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca aaggccagcg
     6541 cggaggcgtt aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa
     6601 ccagaccggt taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg
     6661 ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac
     6721 ttcacccgca cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag
     6781 catagaagcc agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt
     6841 agcactgatt ggcgtaccgt cgataccctg atcattaaca ccaaggatgg cgtaaacaat
     6901 caccaccagc atgatggccg acaaaattgc cggaccaatc cacacctgcg gtttcagcca
     6961 ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa
     7021 cagcaccata atggcacccg cgtagacgat aatttccagc gcaccggcga agtaagcgcc
     7081 cagtgagaag aacaccccgg aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg
     7141 taccggattg gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat
     7201 ataaaaagcg aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc
     7261 ttggcttcgt tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg
     7321 tagaagttat attccgggta tttgcccgga ccggagatca gcagatcctc tttctcgtaa
     7381 accagatcct ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg
     7441 gtcggacagg cttcttcgca cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa
     7501 aattccgggt accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc
     7561 gggcaggcta ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg
     7621 gtcagaacga tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt
     7681 cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg
     7741 ccgaaaccta ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc
     7801 agagaatgac agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc
     7861 agccgaagga cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga
     7921 acatcatcat aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc
     7981 cttgccagcc accgaagaac agcgtcacca tcaatgcaga gatggtcaca atcccgatgt
     8041 actcacccac gaagaacaga ccgaacttca taccggaata ttcaatgtgg taaccatccg
     8101 ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg gtgacatacc gccacgcccg
     8161 cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac gttccacaca tgcgcctggc
     8221 tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc atcagggaaa
     8281 gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc acgcatcgca cccagcaacg
     8341 agtatttgtt gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca
     8401 tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc ggactgactg
     8461 gcacaatcgc aaaggccagc agcagcgagg taaaggcaat catcggtgcc agggtaaaga
     8521 tgacgcgatc cgagaatttc gggatccagt cttctttaaa gaacattttg atcatgtccg
     8581 caaccagctg gagcgaaccg ccccagccaa cacggttagg tccgtaacgg ttctggaaca
     8641 gacccagcag gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca accaccagca
     8701 ggatcaccac cgctttgagg atggtcagca ggatctcaat cagttccggt gatatccaac
     8761 tcattgttgt gcctccttga gatcctcaag atgcgcgcca gccagcaccg gagcaatacc
     8821 ggacataccc atcggcaagc ccacctgccc tgccgtcagc ccttcagcga tttcaaccgg
     8881 cagcgtgacc gtgttgccat cgtaactaaa ggagacgtgt gtacctgcgt tcacacctaa
     8941 cttcgcggca tccgctgggt tgagtttgat gtacggctgc ggcatacggc tctggaagac
     9001 cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg taatacggcg cgatacgcca
     9061 tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccattttc
     9121 gctggtttca aacagacgca cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc
     9181 ctggaatttg ttccacgcct gcggggagtt ccagcccggc gcccaggcaa acggcacttg
     9241 cgaacggtgc gcagtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat
     9301 atcctgcggc tgacgcggct catgaacgct gatattggcg cgcatggcgg tacgaccgct
     9361 gtaacggtgc ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc
     9421 agcatctttg atacctgcca gttccgggat tttcgccaca acagcgtcaa tcacatggtc
     9481 gagctgcgtc cagtccactt cacggctcag cagggtgctg tgcagcgagt gtaaccagcg
     9541 ccagctttcc agcatgacag ttttgctgtc gtaataagca ggatcgtaaa cctggaagaa
     9601 acgttgggcg cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa agctggcagc
     9661 agaaagtacc agatgggcgt tttccataat cgctgtgcgt tgatgatcaa ccaccatcac
     9721 cagcggcgct ttagccagcg cagcattcac gcgggtagcg gaagcgtgac gatgcagatc
     9781 gttttccagc accaccaccg cgtcggcgcg tccggtttcc agttcggtca acgcttcttc
     9841 aagcgaaccg ccacccataa tgcccagccc catgctgttg acggaacggg caatcatggt
     9901 gataccaacg tcagcgccgc gacctttcag ggctttcgcg acgttagccg cagcctgaat
     9961 cacctctatg ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg cacctgccag
    10021 tgcctgtacg atgacgtcga ttttgctttg cagctcaggt tcgataccgt caaccgctgg
    10081 cgcagagtta tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg
    10141 tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa
    10201 cagcggatgc ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc agtcagccac
    10261 tttctgtgct gccgccattt cgcgcgcttt acctttcaca gcctgacgca ctgccagcgc
    10321 gacgcgcgcg ccggtctggg taacgtcttc gcccagcacc agtaccgcat cgtaagattc
    10381 gatttcgcgc agagccggag tataaatgcc gccttcacgc agcactttca gcgccagttg
    10441 cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc ccaccagttc
    10501 acgcagcgca aagttgcttt ccacgctggc acgcggagaa ccaataccga tcactttctt
    10561 cgactgacgc agaatatctg ccgcgccctg cattgcctgt tcggcgttga gggtaatgaa
    10621 atcatcgcca cgacgctgta ctggttgacg cggacgatct ttcaggttga cgtaaccgta
    10681 accgaaacga ccacggtcgc agaggaagta gtggtttacc gtaccgttgt aacggttttc
    10741 gatacgacgc agttcgccgt aacgttcacc ggggctgatg ttacagccga tggaacattg
    10801 ctggcagatg ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt
    10861 tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc
    10921 cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag
    10981 atctgtaccg tcagcgtaat ctttgtagta acgcacacag cggtagcagg cgatgcagcg
    11041 gttcatttcg tgagagatga atggccccaa atcctgatta cggtgggtac gtttggtgaa
    11101 acggtagcga cggaagctgt gtccggtcat cacagtcata tcctgaagat ggcagttacc
    11161 gccctcttca cataccggac agtcgtgcgg gtggttggtc atcaaccact cgaccacgct
    11221 ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg
    11281 tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac
    11341 cgcacactgg cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat
    11401 atcaaggccc agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc
    11461 tttgccgtct acatgaattg tagccattag catgcttcca gttttctcgg tcagggaccg
    11521 agcgttaatc gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa
    11581 tcaaatgggt attgctgaac ggctgtttga ttcccgcctc aaattcttcg cggaaatatt
    11641 tgatggcgct atgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg
    11701 ggcctaagaa tcgacacagt tgctcaagtg tttcgatatc gcccggctga ccttcaccac
    11761 gctccagcgc acgcagaatt ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc
    11821 agccgcagga ctcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga
    11881 tctcatggtc aaccgccatc gccagcgccg tacccagacg gcttcccgct ttaccgatac
    11941 tttcgaattc catcggcagg tcaaggtgcg cttcggtcag gaagtcggtg cccgcgccgc
    12001 ctggctgcca ggctttgaat ttcagaccgt cacgcatacc accggcgtaa tcttcgagga
    12061 tctcgcgcgc ggtagtacca aacggcagtt cccacagtcc cggatttttc acccgaccgg
    12121 agaagcccat cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca
    12181 cgccgttagc gaggatcgcc ggaacgttac acagggtttc gacgttgttg acacaggtcg
    12241 gtttgcccca tacgccggag gttgccggga aggggggttt cgagcgtggg ttagcacgac
    12301 gcccttccag ggagttgatt aacgctgttt cttccccgca gatgtagcgc cctgccccgg
    12361 tatggacgaa cagttcgaag tcgaaacctg ttcccataat gtttttgcca agcaagcccg
    12421 cttcagtggc ttcggcaatg gcgcggcgca gatttactgc cgcttcgata tattcgccac
    12481 gcaggaagat gtagccacgg taagctttca gcgcaaacgc ggagatgagc ataccttcca
    12541 ccagcaggtg cggcagttgc tccatcagca ggcggtcttt ataggtgcct ggctccattt
    12601 catcggcgtt acacagcagg taacggatgt tcatggattc gtctttcggc atcaggctcc
    12661 atttcaggcc ggtagaaaag cccgcgccac cgcgcccttt cagaccagcg tcttttacct
    12721 gattaacgat ttcgtccgga gacagcccgg tcagcgcctt acgcgcgcct tcgtaaccgt
    12781 ttttgctgcg atattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca
    12841 gcggatgcgt ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg
    12901 gatcgcttcc ggggtcagat gcgcgtgagt atcctcatcg atcatcatgt ttggcccttt
    12961 atcacagttc cccaggcagc aagttggcag cagcgtaaag cgaccgtcaa atgtcgtttg
    13021 ccctggtttg atgtttagct ttttctcgag cgccgcctga ataccctggt aaccgttgat
    13081 atgacagacc acgctgtcgc aataacggat cacatggcgg ccaaccggct ggcggaagat
    13141 ctggctgtag aacgttgcca caccttcgac gtcgcttgcc ggaataccca gcacatcggc
    13201 gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc ttctgaacga ttttcagcgc
    13261 ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg
    13321 ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag
    13381 cggtccacat ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc
    13441 aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtgcggata
    13501 cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt agtaactgtt gatccccttg
    13561 gtcgcctcaa tcatctggaa agattcattg gcaggcatca ccggccccca cgacacttgc
    13621 aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg
    13681 gtcagcgggt gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga
    13741 atgcgcagac tctggcgcag ctcttccact ttcagcatta cgcgggtgta gcagtcagaa
    13801 acaccaccac caaccgggat ttcaaagtcg aagttttcat agccagaata aggacgcgcc
    13861 ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg cgccagtggt gccccactcc
    13921 agcgcctctt tcgcgccata ggcggcaacg ccctgggaac gacctttcag aatggtattt
    13981 tgcagcgccg ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc
    14041 agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc
    14101 gggtgcatac ggaaacccgt gatcgcttcc accagatcgt aaattttctg acgatcggta
    14161 aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa taaaggtcga gatgtacagc
    14221 aggtggctgt tgatacggaa cagttcggag agcataacgc gaatgacgtt aacgcgatcc
    14281 ggcacggtga tcccggccag tttctctacc gccagcacgt aaggcatttc gttaacgcag
    14341 ccgccgaggt attcaatacg gtcggtatac ggaatgtagc tgtgccagga ctggcgctcg
    14401 cccattttct ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc gacaatctct
    14461 tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg ccgacgggtg gttcggaccg
    14521 aggttgagga acatgaagtc ctcgttttcg gtgccgcgct tcatccccca ctcttccggt
    14581 ttgaaggtca gggcttccat ctccagatcc tgtttggctt tggtcagctc aaacggcgag
    14641 aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca ggtttgcggc
    14701 atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt
    14761 tcacgctcat accagttagc gttcgggaac agtttggtga aggtcggtac gtgcaggtcg
    14821 ttttctgcca gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg
    14881 tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc
    14941 atgccgtgta agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgcca
    15001 acttccagta attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga
    15061 acagtaaagg catccggccc aaaacggttg cgcagttcgc caatcaccgg atcatcaaga
    15121 tgatcgcggg tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt
    15181 gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag
    15241 cgaagaaaaa tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg
    15301 gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa
    15361 acgccctgat cgccaaccac ccaggagagc ggacgacgtt ctttgccgat agattcctgc
    15421 aacagcatca gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca
    15481 tcaaccggga tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccacca
    15541 gagttggcac aggcacccat tgagataacc cattttggtt ccagcatctg gtcatacaga
    15601 cgctgaataa ccggtgccat tttggtaaag caggttcctg caaccaccat caggtcagcc
    15661 tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta
    15721 aacgaagtca ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag
    15781 tttttacgac cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact
    15841 tcttgctcca gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca
    15901 ccgttgggat ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga
    15961 cgattagcga tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc
    16021 cagtccagcg cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa
    16081 attgcagctt ccacaaagcc tacccagccg ctttcgcgga tagaggttga ccatgcgaac
    16141 agatacagcg cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg
    16201 gacaggcgta agcgggcgga gccgaccgag tcgataccgg attcaaacgg cacgtttttc
    16261 gacctcgcgc gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg
    16321 ccaatggcaa cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat
    16381 gttgacatac tcattgctta ctcatcaaaa gtagcgccag attcactgct cttcacggca
    16441 gatggacgcc acatcgattc atggggagga ataaaaaaaa ccttacaatc actgtagaaa
    16501 ttcttttata cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt
    16561 taacaaaagt ttcttcacat tagtttacat aatatcaaca ccattagcat ttaatgccct
    16621 ttcaccccag atccttgacg actccaggat aattagatgt tgttgaatcg tgtccgttgt
    16681 gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca
    16741 aaagagagta ttttcttgat ctgtgacacg cttttgtcat tccataacaa aaacgcagca
    16801 acaaatttac gtatttttta acatcattgt agcaggtgat ttttttcagg cgattatttg
    16861 tgcgttcggg acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact
    16921 atgcgaggaa ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct
    16981 cgttttttac actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt
    17041 tcaatcaaca atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta
    17101 tggtagctct ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca
    17161 tagcacagca aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca
    17221 cgcaggtccg ggctcatcat ctcaaccggc cttgccgtca cgccaagacc ggctttcact
    17281 gccgcacgaa cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct
    17341 ttattaagcg tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga
    17401 gggatcggct cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag
    17461 gttcgcaggt tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact
    17521 tcctgggatt ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct
    17581 aatttcggat aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat
    17641 tcatctgaag caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag
    17701 gcctcatcat taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca
    17761 gttaacagtt tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga
    17821 cgctgcattt gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca
    17881 aaagtgttca gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt
    17941 atcggacgat ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt
    18001 aatgctgtgc acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat
    18061 ccggaacccg gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg
    18121 ttccgttata atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta
    18181 agttactcac tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc
    18241 cttgttccac tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt
    18301 gaggcggcat caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt
    18361 tatttataag ggtcattgcg aattatctga tgcaaagtta tgttatgtta ggcaaagtaa
    18421 tcttctatta ttaataagca catcaaaacc tttttgaata ttaaataata attaattagc
    18481 atcatcctca ttcattaatt ccgcttaaca atagtttcac accttgcccc cattacgaca
    18541 cctcggaaat catcacgctg ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc
    18601 attagtgaat tcttaaatga ggcattttca cacaattatc ttacagataa aaaaaccaga
    18661 cttacaatta agaatcagaa caattcacta tataacattg catgtaaagc atatacacct
    18721 cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat
    18781 cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc
    18841 cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa
    18901 tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa
    18961 cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc
    19021 ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa
    19081 gggtattgcg attccaaagg tctttactcc gcgcgtaaag ccatcatgca gcactaccag
    19141 gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag
    19201 cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca
    19261 ccagattacc cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat
    19321 ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg
    19381 cctcgtacgc gtgggatcgt tataatcaac ccaaataacc caaccggcgc ggtatattcc
    19441 aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc
    19501 gatgaaattt atgacaaaat tctttacgac gacgctgagc atcactcaat tgcgccgctg
    19561 gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc
    19621 ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa
    19681 ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt
    19741 cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt
    19801 tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg
    19861 aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac
    19921 gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg
    19981 acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat
    20041 gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa
    20101 tcttaatttc actgccggag attgcatccg gcagcgttat cccgccacaa tgacctgatg
    20161 atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc
    20221 tgaaactcat taaccgctgg ccgctcatgc gcaacgtgcg gacggaaaat gtgtccgaac
    20281 acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cggaaatttg
    20341 gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag
    20401 tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt
    20461 acaaggctat tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc
    20521 gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg
    20581 tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa
    20641 ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc
    20701 aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga
    20761 ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc
    20821 ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac
    20881 cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt
    20941 tcccggacct aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga
    21001 tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc
    21061 agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat
    21121 aaccgccgac aagacaaaca aacaccccag catcatatgt ggcccgtaac caccgccaat
    21181 gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa
    21241 cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc
    21301 agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt
    21361 aggaatttca tctgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg
    21421 tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa
    21481 gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat
    21541 atcgcccagc agcagaggct catccaccag cgaaccttcc agcgccacgc cattgcgctt
    21601 tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc
    21661 aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc
    21721 agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg
    21781 gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga
    21841 aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac
    21901 gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccta tcattggcga
    21961 tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc
    22021 gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat
    22081 gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt
    22141 tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag
    22201 gttcggcggc gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg
    22261 catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat
    22321 aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat
    22381 caacaaaacc aacattttga tttcactgtt gcccgccact ttgaccagcc atgttcccat
    22441 tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag
    22501 gacaacgtta ggatcagaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa
    22561 tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa
    22621 caagacgatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta
    22681 tccttgttaa ttgaggggga tgacttgatt ctgccataaa aaagccccga cgagacgggg
    22741 ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca
    22801 gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc
    22861 ggagcgttaa ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga
    22921 gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc acctaacaga
    22981 tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt
    23041 gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca
    23101 atggcccacg gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc
    23161 gcggtaatac cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca
    23221 atatcagcct cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg
    23281 tgaatgaaag ccagcacctc ttccggcgct aacccatgac gtctggccca gttgctccac
    23341 gcccgttcta ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct
    23401 ttgcaccgca cgcgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa
    23461 atggtactga cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat
    23521 tggcgtctgg gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac
    23581 attaaccata aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag
    23641 gaaggtgaca cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg
    23701 caacgcatga tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc
    23761 gccaaactcg cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg
    23821 caggcgacgg taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt
    23881 agacaaaata agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactctact
    23941 acggtgaaaa aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt
    24001 acaaatcatc aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta
    24061 atgcctggta atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt
    24121 taccgcgaac ttcataaaac cagttgagga tagcaggggg taatggcgtt acagaacgct
    24181 tgcccagcca ccataatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg
    24241 ccggcccaag ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg
    24301 gcataaaacg gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg
    24361 caaggcgttt ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa
    24421 aaaaattaac agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag
    24481 attcaaaaat ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc
    24541 catacccact atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga
    24601 gtcgtcaaat tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc
    24661 aagatatttc ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc
    24721 ggtgtcatca tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta
    24781 taggtacttc catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga
    24841 aatttgccat catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt
    24901 tccacctgcc tgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt
    24961 taggtgcagg cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac
    25021 aaaaaccaga actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg
    25081 aaaagtatac cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag
    25141 cttcttttgc accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat
    25201 ctttcccaca gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta
    25261 tgccggaaga gtcttacctc tacgccctgc cttacaacct gtacaaagag cacggcatcc
    25321 gtcgttacgg cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc
    25381 tgaacaaacc ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg
    25441 tttctgctat ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag
    25501 gtctggtcat gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg
    25561 acaccctggg catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc
    25621 tgggtctgac cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag
    25681 aagacgcgaa gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct
    25741 acactgcgct gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa
    25801 atgccgcgat ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg
    25861 atcatgaacg caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta
    25921 cccgtcctgc ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc
    25981 gcctgactgc ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca
    26041 aatcggcggt aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg
    26101 aaccagcgtc ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg
    26161 cgttcgtctg agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga
    26221 tcagactacg actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa
    26281 aatgagctac gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat
    26341 cgtcgcaaac taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt
    26401 cccgacacgt aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa
    26461 tgcggaaatc gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg
    26521 tatcgaactg acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat
    26581 cgttaacaaa ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga
    26641 gattttcgac gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga
    26701 atccagcccg ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg
    26761 tgcgatcgat atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac
    26821 tcgccgcgtt aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt
    26881 ccgtgccggt tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tggccgcttg
    26941 cctggcagcc atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat
    27001 ggacgcgcgc atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat
    27061 ggtgaacacc aacacctggc agacctctct gagcctgcag agcttcaacc tggaagttcc
    27121 ggttgacgat cacgaacgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc
    27181 tgactggatc gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc
    27241 gttccgttat cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga
    27301 aggtgacgaa ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac
    27361 ttgcgtactg ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga
    27421 actgggtgca gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg
    27481 tctggtcgaa ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga
    27541 agacaacgtg gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc
    27601 cggtgctgtt cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc
    27661 accgggcagc tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt
    27721 ttacggtgac tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat
    27781 tcagtccgct gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta
    27841 ctccaccggt acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct
    27901 ggcgcaggaa aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt
    27961 aatggctgac gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt
    28021 gttcatcttc ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc
    28081 cgacctgatc tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc
    28141 ccgtggcgca ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc
    28201 acagcagcag taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa
    28261 taatcactat ttccggtttt ttattctctt aatctgcatt aatcctttct gattatcttg
    28321 cttaactgcg ctgcatcaat gaattgcgcc atttcacttt gcatacttac cactttgttt
    28381 tgtgcaaggg aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg
    28441 ggcaatgccc gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac
    28501 ctgggtagtt ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca
    28561 aacaaaaaca cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg
    28621 cgaacctgag tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat
    28681 gaacttcccg tttgaaggat taacctcagg atcgaaatac gggacagctg ttggcatcat
    28741 catgtttatg ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga
    28801 taacggtatc ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc
    28861 gctgtttatt ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc
    28921 ctttgccatt atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt
    28981 cctggtgacc tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg
    29041 tgtggtcgtt gctcagggga ttgccggcgt tccggtgctt tctggctccg agttgcgcat
    29101 cgtggtgtgg gttatcgcca ctatgattgg cctgatcttt accatggtgt acgcctcacg
    29161 agtgaaaaag aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa
    29221 gcaggcggat gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct
    29281 gaccgccgta atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga
    29341 aattgccagc cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg
    29401 ccttaacggc atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat
    29461 gatcgcccct gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg
    29521 tgaagcgggt gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag
    29581 cggtctggac aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt
    29641 cttcgtgacg tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg
    29701 cgatctggtc ggtgttaacc gtcaggttac cgtgctggca ttccagtttg gtgatggctt
    29761 cagtcacatc atttacccaa cctcagcttc gttaatggca actctcggtg tatgcagggt
    29821 ggacttccgt aactggctga aggtgggcgc gaccctactt ggactgctgt ttattatgtc
    29881 cagcgtcgta gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca
    29941 attgcggctc ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg
    30001 cttcatccac aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt
    30061 gatttcttcc ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg
    30121 accgtgagag acgcagctga acaacgcgcc ccagacacgg caatttttat cttcgaaata
    30181 gaactgcccg tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg
    30241 acgcgcggat tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa
    30301 catgccgggt aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc
    30361 atcatgcacg acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg
    30421 gctggcttgt gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc
    30481 caaacgacgc tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt
    30541 tgagttgtgg gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag
    30601 tgcgattaca ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt
    30661 cggaatactc accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg
    30721 cagatgggta tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg
    30781 gccaaaaaga tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca
    30841 cggcgcggtt atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg
    30901 cacagcgata accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta
    30961 accctccggt aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca
    31021 ctgggcaccg ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg
    31081 aatgtccgat gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg
    31141 atactggatt ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct
    31201 ggacagacgc tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat
    31261 cgccatgtag taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt
    31321 caccaaataa attcggctga cccagtacta acaaatgttc tgccatcgca aacagtttct
    31381 ctgcactggc ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat
    31441 ccgtcggacg ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc
    31501 gtgcacgcgc acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa
    31561 atcgatcttc cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt
    31621 gtaataacgg cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac
    31681 cgctgtcgag gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc
    31741 aggcggataa cacataaggg gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt
    31801 tactcattat gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc
    31861 actgtcacgt gacgaatact cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga
    31921 tgattaaaca accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca
    31981 caaaattacg ctgtttctcg aagaagcaga gcttgattat cgcttgatta aggtagacct
    32041 ggggaaaggc ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc
    32101 ggcaattgtt gatcattctc ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg
    32161 tgccattttg ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga
    32221 gcgcgccgcc acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg
    32281 gcaaaatcat cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta
    32341 tcaggttgaa actcagcgtc tttaccatgt actgaacaag cggctggaaa actcgccctg
    32401 gctgggaggc gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg
    32461 gactcgccag cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat
    32521 ccgttcgcgc cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc
    32581 ggatagttaa cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa
    32641 cctgcaatgg cacaacctgc cgctattatt cgtataaaga accttcgttt acgtacgttt
    32701 atcggaatta aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc
    32761 cactaccccg ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc
    32821 accgtaacga aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa
    32881 ttaactcagg atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg
    32941 gagatcgata aactgcacgc gctgcgctac gccgattcgg tatccatgac cttaagctgg
    33001 cagcgttaac cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat
    33061 taattggtcg ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc acggtagtga
    33121 cgcgtaaccc gcagaaagcc agttccgttc tcggccctcg ggtgacgcta tggcaagggc
    33181 tagccgatca aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga
    33241 ttgctgataa acgctggact cacgagcaaa aagagcgtct ctgccaaagc cgctggaata
    33301 tcacgcaaaa actggtcgat ttgattaatg ccagcgacac gccaccgtcg gtactcattt
    33361 ccggctcagc aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac
    33421 cgccgcataa cgaatttacc cataaactct gcgcccgctg ggaagaaatt gcctgccggg
    33481 cgcaaagtga caaaacgcga gtgtgcctgc tgcgtaccgg cgtagtgctg gcaccggatg
    33541 gcggtattct cggtaaaatg ctgccgccgt ttcgtcttgg cctgggcggg ccgattggtt
    33601 ccggtcggca gtatctggcc tggattcata tcgatgatat ggtcaacggc attctctggc
    33661 tgctggataa cgagctgcgc gggccattta atatggtttc gccctacccg atacgcaatg
    33721 aacaatttgc ccatgcgctc ggtcatgcgc tgcatcgccc ggctattttg cgcgtccccg
    33781 caaccgccat tcggctgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg
    33841 cgctgcctaa acggctggaa gaagcgggtt ttgcgtttcg ttggtacgat ttagaagagg
    33901 cgctggcgga tgtcgttcgc tgatgtggtt tactgcaaac atccgccagt taactcccgg
    33961 tgttacaggg ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca
    34021 gaactaactc tctgtctaaa cctctatcca gcatctcctg agcaatacgc agggcttctt
    34081 cgtgtttgcc ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc atcagtttct
    34141 ccttttcttg tggtgcgcgt tctgttatct caccaataaa tgcacggaaa cgctgggcat
    34201 cccctgtttg taatacgtaa ttaaacaggg cttttagctg tctgtcatta gtgttccctg
    34261 taactagcag cgaaacaatt tggtcgacta atcccaacag atcgcgctga cgaatatgtt
    34321 tctgaattaa ctccaacagc gccattttgc ggtgttgcat aatctcgtca tccggcacca
    34381 cggtaatatc caccaacgga aacgctgatg aatatatttt gcgggctata gcaggctcgg
    34441 caaattcatc aagccagcag agtgaataag gataaggact tctgcaacca tgataaaaca
    34501 gcatcgggat caccaatgga agctctttat agcccgcatc aagatggttt tgcattgccg
    34561 caatggaata acgcatcatg cgaaaagcca ttaattcttc cggcttactt tggtgctcta
    34621 tcactacata aatataaccc actccctcct gcgttttcac agaccacaag aggtcggaat
    34681 aatattgccg caggtcttca tcaataaaac tgtttggttc cagtttaagc gtcgttaaat
    34741 cacacagttt gcgcagcggc gcgggaagat gaatatcaat aaaatcccgc gcggtgtctg
    34801 gatggcgtaa aaaagattta aataccgcat catgcggcgt ggaagttgtc gatattgtca
    34861 tggcgttccg tcacccctca aatttaagat gacgcgacaa taaccacgat taacacccaa
    34921 tgcagcaggc agtttttatc tttggacagc gaattcagaa gtatttttag cggactgcaa
    34981 cttttacatt tcagcggaag ctgactcgca aattccccat gaaaatactc cggcagcgtt
    35041 agccaccgga gaggaaatta tttcagcgat cccttaagga actgttgcag acgagggctt
    35101 tgcgggttgc caaataactg ctccggcgcg ccctcttctt ctattttccc ctgatggagg
    35161 aaaatgacat gagtagaaac atggcgagca aagcccattt cgtgagtcac taccaccatg
    35221 gttttcccct cttctgccag ttgctgcata atacgcaaca cttcgcctac cagttcagga
    35281 tcgagcgccg aggtaggttc atcaaacagt aaaacttccg gttccatcgc cagcgcccgc
    35341 gcgatagaaa cacgctgttg ctgaccgccg gaaagatgca ccggatattt cccctgcgca
    35401 cgttcgtcta tcccgacttt tgccagatac ttgaccgccc gctcgcgcgc ttcctgcttg
    35461 ctcaggccca acacctgaat cggcgcttcc atgacgtttt ccagcaccgt catatggctc
    35521 cagagattga agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt
    35581 ttatcggcga ctttgagttg accgtctttg tcgcgcacca gattgatcgt ctggccattg
    35641 accacgatcg acccttcact cggtttttcg aggaagttaa tgcagcgcag aaaggtactt
    35701 ttccccgatc ccgacgatcc gatgatgctt attacatctc cggcattcgc ttgcagtgag
    35761 acccctttca gcacttcatg ttcgccgtag cgtttgtgca aatcgataac gtttaattta
    35821 ttctcggaca tcgtattctc agtgcgttga agaaggtttc acatgctgca accagcgttt
    35881 ttccgctctg cgaaagaggc tgatcaggac ataagagatg attaaataga gcaccgcggc
    35941 aatgccgaag gcggtaaaag gttgatacgt ggcggcgtta atatcgcggg ctattttcag
    36001 cagatccggc acagtggccg taaacgccag cgcagtagag tgcagcatca ggatcacttc
    36061 gttgctgtat gccggtaacg caatacgcag cgcagaaggc aaaataatgc aacgatacat
    36121 tttaaaagtc gagaagccat aggctctggc ggcttcaatt tccccatgcg gaaccgaacg
    36181 gattgcccca gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac
    36241 ggtacagttc aggccactgc ggaagaaagc gttaaggaat tcggttccct taacaatctc
    36301 aagcgtgtac atgccggaat agaacaccag caactgaaca tacagcggcg taccgcgaaa
    36361 aatataggta aataaccaga ttggaaactg gatgtattta ttactggaga cacgaccaat
    36421 cgccagaaac agcgccagga ctccgcctat cactaccgac aaaataagca gccacagagt
    36481 gatcgccaca ccagtaaagc gataaccgtc ggtccacagc agcggtttcc agtattcatg
    36541 taagatttcg atcacaggtc agccctcttc acacccacgg agtagcggcg ctcaaggaac
    36601 agcagcacac cattggaaac agtggtgaaa accaggtaaa tcacgccaca gacgatggcg
    36661 aaatagaacg gttcccaggt acttttgcct gccagttggg tggctttgac cacatcttcc
    36721 aggccgagta acgaaaccag tgcggtagat ttgaggatca cctgccagtt gttgccaatg
    36781 cctggcaggg cgtaacgcat catcgccgga aacatgatcc gccgaaacac ttgcccacga
    36841 gtaaaaccga acgccgtcgc cgcctctata tgccctttcg gcactgccat aaacgcgcca
    36901 cggaacgttt cggtaaaata agcaccgtag ataaaaccga gagtaataat accagcgacc
    36961 atcggatcga tatcaatctg cccgacgccc atcgcctccg tcaccgtgtt tagcgcaatc
    37021 tgcaaaccgt agaaaatcag cagcatcaac accaagtccg gcacgccacg aatcagcgtg
    37081 gtgtaccctt cgaaaatcag ccccgaaagc cgattttgcg agagcttacc gccagcgcca
    37141 attaaaccga tgattacagc gagcactaca gaactgatag ccagctccag cgtgacgagc
    37201 gcaccctgta aaataacacc tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg
    37261 gtgaattacc gcctcttgtc tccctccggt tacccgaagg gagcgggctt tctttcaccg
    37321 atggcgatta accgccataa acatcaaaat cgaagtactt tttcgctaat ttctcgtaag
    37381 taccgtcagc gcgcatttcg gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt
    37441 ctttacgcag gcccatgccg gtccctacgc caaacagttt ttcatcttta acagacgggc
    37501 caccgaattt gtaatcttta ccgacgggtt gtttgaggaa accttcactg gcagcgacct
    37561 catcctggaa cgcggcatca atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc
    37621 cctgatacga gacgatttca atgccttttg gtgcccagtg ttcgttaccg aacgtctcct
    37681 gggtggtgcc ctgcaatacg cctacccgtt tgcctttcag cgactcgact gtcggctgaa
    37741 tgtcggaatt tttcgccacc accaaacgag aatcggcagc gtacagtttg tcggtgaagg
    37801 ctatttcttg ctgacgtttt tccgtaatgg aaagcgatga catgatggcg tcaatcttct
    37861 tcgcttttaa ggacgggatt aacgcatcca gcggattttc gacaaacgta cattgcgtat
    37921 tgatgcgttt gcataattcc tttgccagat cgatatcgaa gccaaccagt tcgccttgtg
    37981 aattttttga ttcaaatggc gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa
    38041 tcgcagcaaa tgccgcagtt gcgctggaga aggccagaac cagagagagc gatagcacca
    38101 gttttttcat aatgtcctca actgacagtc tttttatagg gaatatttac aggtttgtta
    38161 cacttatcgt gccataaaat tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt
    38221 atttttcatt aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg caacaaacct
    38281 ggcgtgcacc atgatgatca ttccatcagg tacagcttcc cagcgacgta tcagtcaccg
    38341 tagacattaa agtcgaaata ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc
    38401 tcgccaagcg ccttattgaa ggcagccgtc agttcagcat catctttacg tagccctaca
    38461 ccggtgccat caccgaagta ttttttgtct tttactgatg agccagcaaa ggcgaaatct
    38521 ttaccagcag gttgcttgag gaatccttcg ctggcagcaa cttcatcttg taacgcagca
    38581 tccagacgtc ctgcagccag atcggaatag accaaatcct ggttggcata ggccaccaca
    38641 tccacgcctt tgctacgcca ggtctcgtta gcgtaagctt cctgggttga tccctgcagc
    38701 acaccaacat gtttaccttt cagtgaatcc agcgttggct gaatcggtga acctttggca
    38761 gcaatcaaac gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt
    38821 ttatcggtaa tggaaagcga cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg
    38881 atcagcgcgt caaagtcact ggcaacccag gtacatttca cctgcatccg tttgcacatc
    38941 tcgttaccga gatcgatatc aaagccaaca aaatcacctt tagcatcttt cgacgagaac
    39001 ggcgcatagg tggtatcggt tccgatacgt accgtctccg gtagcgccgc atagctggaa
    39061 gccgctgcgg agagaccgac taacaaagac agagcgagaa tcgacttctt catacataac
    39121 cctcaagtga aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc
    39181 atgcaggtct tatgccatct tgacgacaac agtaacattc aacgttaaat atgttaataa
    39241 gacgttgcat tattgtcctg aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt
    39301 tcgattgaat ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa attgccctga
    39361 aacagggcaa cagcggagtt atgcgccctg ccagcgggca aagagatctt caggaagggt
    39421 tatcgcaaac tggtcaagaa cacgattaac cgtctgattt atcacatcat caagggattg
    39481 cgggcgatga taaaacgccg gaacgggagg cataatcacc gcaccgattt ctgccgcctg
    39541 agtcattaaa cgcagatggc ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg
    39601 gcgacgctct ttcagcacca catctgccgc acgggtcagt aaaccatcgg tgtagctatg
    39661 gacaatgccg gaaagggttt tgattgaaca gggtaaaata accatcccca gcgtctggaa
    39721 agaaccggaa gagatgctgg cggcaatatc gcgcgcatcg tgcgtgacat cggctaatgc
    39781 ctgcacttcg cgcagagaaa aatccgtttc gagggataag gtctggcgcg ctgcctggct
    39841 catcaccaga tgcgtttcga tatctgtgac atcgcgcaga acctgtaata agcgcacgcc
    39901 ataaatcgcg ccgctggcac cgctgatgcc tacaatgagt cgtttcataa atagttgccc
    39961 tgtcagactt gcaggcagac tttgcaggat ttcggcggga gttgcaagtc agggtgccag
    40021 accggcaccc tcagcgaagg catcatcctt cgttatgcat ttcgagattt tccacttcgt
    40081 tctgacgttg cactgctttg gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc
    40141 cctgatcaac atctttggtg acgtagacgc cgttgaacac cgagcattca aactgctgga
    40201 tatccggatt ttcagcgcga acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc
    40261 cgtcagcacc gatgatctgg cgaatttcat caacttcgcg accgtgagcg ataagctccg
    40321 tggcgctcgg catatcaata ccataaacgt tcgggaagcg aatttccggt gccgcagaag
    40381 cgaggtacac tttcttcgct ccggcttcgc gtgccatctc gataatctgc tcagaagtgg
    40441 tgccacggac gatggagtcg tcgaccagca ggacgttttt atcgcggaac tcggcgcggt
    40501 tggcattcag tttacggcgc acggacttac gacgcagctg ctggcccggc atgataaagg
    40561 tgcggccaac atagcggttt ttaacgaagc cctggcggta cggtttgccc agaatacgag
    40621 caatttccag cgcgatatca cacgaggttt ctgggatcgg gatcaccacg tcgatatcca
    40681 gatcttccca ttcgcgggca attttctcgc ccagtttcgt gcccatattc acacgcgcgc
    40741 tgtaaacgga aattttgtcg atgaacgagt ccgggcgagc aaagtataca tactcaaaca
    40801 ggcacggatt gctgaccgga ttgtcagcac attgacgggt aaacaactgc ccttcttcag
    40861 tgatgtaaat cgcttcgccc ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga
    40921 gcgctacgct ttcggaagcg accatatatt ctgtacggtt ctcgtcaata tcacgttttc
    40981 ccagtaccag cggacgaatc ccgtttggat cgcggaaagc aaccataccg tggccgataa
    41041 tcatcgccac acaggcatac gcgccgcgga ttaagcggtt tgtggcagca atggcagcga
    41101 aaatattgtc ggcttccagc gggtagtggc ggaaattgtc cagctcgctg gcgaagatat
    41161 taagcagaat ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt
    41221 ttttacgcaa ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta atgccatacg
    41281 gggagttaac gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc gtagggtaac
    41341 gcacatgacc aatgcccata ttgccctgca aacgctgcat atggcgagct tcaaatacat
    41401 cgctcaccag cccgttcgct ttacgcaaac ggaagcagtt attggcatct atggtgatga
    41461 tgccggcggc atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa atcgactggt
    41521 taaccggcat aacaccggcg ataccgacaa taccgcacat acgtcttttt cctcgttaag
    41581 ccacatctca gagcacttac gctctgggca agaaacttga cgagctttgc agataatcaa
    41641 aaaaccatct gatgataaaa ctgaattgcg ggatcagctg tgatttgctc cagtcttcgc
    41701 ttttcgacac cccggtaaag gagtcgagaa agaagagaat ggcggcaaca atcaacacac
    41761 cgcgcaacgc accgaaacag acgcccagca cccgatcggt gcctgacaac cccgttttct
    41821 ccaccaactg gcctatcacg aagttcacga tagcaccaac gatcagggta gcgataaaca
    41881 gtaccgcgat ggcaatccca tttcgaacca gttcgtcttc aaagcccgta aaccagactg
    41941 acaggtaagt gtagtaatga ctggcaacaa agaaagcaca accccatgtc accagcgata
    42001 acgcttcacg aacaaagccg cggatcaggc taaccagaga ggaaaaagca atcaccgcga
    42061 ttatggcgta atcaatccag accatatgtg tcccacgatt ttacgccctg tcatcctgtt
    42121 cggggcgcat tctaacagaa aaagaaaacg tttgcgtagg gatttccttc ccacgcatca
    42181 ataaaaatgg cgctgaaaaa atattcaacg ccatcgactt tttatgcctt tgcggcatcg
    42241 tgcaatgcgt gtcggatgcg gcgtaaacgc cttatccgac ctacggttct aacactgccc
    42301 aggcctgata agacgcgcca gcgtcgcatc aggcaagact gtattaattc ggcgtatagc
    42361 ccattaccac gccacttaag ccagaaagtt gcttcaactc acccagcgaa cctttcagct
    42421 tatctttcga ggcatccggc ccaaccagaa tacgggtaat tttaccctgc actggcgtgg
    42481 atggcgacgt ataaacccga taaccggcac cgcgcagctt accgacaatc tcattcactt
    42541 tatcggcatt tttcagcgca cccagttgca caacataggc tttacccgtc ggtgcagctt
    42601 tttcttccac gaccggcttc ggttctggct tcggcgcagg tggtgcttca acctttggtt
    42661 taggcggctc caccggtttc ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact
    42721 cggtgttatt ggctgcaata gtggccggat cgagcgacgg tgctgcggca tcacctgccc
    42781 gcacctcttc cgctgcgcct tccggcggct gcgtcggcaa cgcctgggtg gcggctggca
    42841 tcatatcagg ctcatcgcga tcgcccgctt ttggaaccag cgggatagcc gcaaactcat
    42901 cctgataatg ttttttctgc ccgtccagca gccctggaag tacaatcacc cccagcgcca
    42961 ccagcacgat cgtgcccact aaccgattct gaaacttact tgccaccgct tctcctcgcg
    43021 tcaatcactt ccatgacatg tgcgaccgtg tggaaagaac cacacaccag cacggtgtct
    43081 tccgctttag cgtccgccat tgcggcatcc catgcctgcg caacgctatc aaatgatttg
    43141 ccgttaccca aatgctcaag cagttgttct gccgtggcac cgcgcggccc ttccagtggc
    43201 gcacaatacc agtcatcaac cacgcttttc aaccaggcca gagttccggc aatatcttta
    43261 tcatgtagca taccgataac cgccagcacg cgcccgtttt tcggtaaggc tttcatacgc
    43321 ccggtgagat attccgccgc atgtggatta tgcgcgacat caaaaataac gcgtggcgac
    43381 tcgctcacaa tctggaaacg tcccggcaaa attgcgctgg caatcccgtc gcgaatggca
    43441 ttttcactga cctccagccc gctggcacgc agtgccgcca gcgccgttgc ggcattcggt
    43501 tgcgggacaa gcggcaacgg caaattttcc agcgtgccgt gagcatcgct aaacgtccag
    43561 tcatgatcgg tgacggaata gttccactca acgccccgac gttgtaacag tgcacctttt
    43621 tcctgcgcca catcagcaat ggtagaaggc atttccggct caccgacaat tgccggtttt
    43681 tcgctgcgaa agatgcctgc tttctcgcga ccaatacttt cgcgatctgg ccccagccag
    43741 tcggtatgat ccagcgcaat gctggttact accgcgacat cggcgtcgac aatattggtt
    43801 gcgtccagac gaccgcccag ccctacttcc agaatcacca cgtcaagttg tgcctgtttg
    43861 aacagccaca acgccgacag cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt
    43921 gccgattcaa tctccgcaaa agaggcggtg tgggccgatt ccggcaattc ctgaccctgc
    43981 acacgtacgc gctcggtata acgcaccaga tgaggcgaac tgtagacgcc cactttgtac
    44041 cctgccgcca tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc
    44101 gcaacggtaa acacaaatgg cgctggtttc agaacgccaa gacgcgccgc gacctggctc
    44161 acgcgctcaa ggccgagatc gatagtttta ctgtgcaggt tttccagata agaaagccac
    44221 gaagccagag gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc cgctgattcg
    44281 ttacggtgag aatagcaaaa gggcagagcc agtggccctg cccttatcag ttatcaggcc
    44341 tcaggttcct gatccggtac cgggggtacc actacgcctt cacgcggcgc ttcaggattc
    44401 ggcgctggca gattcatcaa cttcgccaga atgctcgcca gtttcaggcg catttccgga
    44461 cgacggacga tcatgtcgat cgcgcctttc tcgatcagga attcactgcg ctggaatcct
    44521 ggcggcagtt tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa gccgattaac
    44581 gctttcggtt cagcgatgtt gagatcgccc agcatggcga aacttgcaga aacaccgccc
    44641 atcgtcgggt cggtcagcac ggagatgtac ggcaagccgc gctcctgcat ttttgccagt
    44701 gccgcagagg ttttcgccat ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca
    44761 ccagaggcgg agaagcagat cagcgggcag ttatcttcca gcgcctgctc aacggcacgc
    44821 acgaaacgtg cacccacaac agaccccatt gaaccgccca taaaggcgaa ctcaaatgcc
    44881 gcagcgacaa ccggcattcc atacagagtg cctttcatca ccaccagcgc atctttttcg
    44941 ccagtttctt tctgcgcaga tgccagacgg tctttatact tcttggagtc acgaaacttc
    45001 agcacatctt tcggctcaag ctcgctaccc agctccacaa ggcttccttc atctaacagg
    45061 ctatgcaggc gattacgcgc tgtcatacgc atgtgatggt cacacttcgg acagacctca
    45121 agattacgtt ccagctcagc gcggtataaa acctgaccgc agctatcaca cttagtccac
    45181 accccttcag gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc
    45241 cagctcatta gggacctttc tgtctgaacc tggttcgatg ccagttttat ctttggggac
    45301 gcataatgcc atttttgccc ccaacagacc atgaatgttg cacattaaaa cataacagcc
    45361 cgaaactttg gataaaaaag tggtcgaacc gcggagttac tttttatttt gcggcgcgtg
    45421 ccgcagcgcg tttgtgacga ataaattcga aaaccccagg caatattgaa acaaaaataa
    45481 tgccaacaat catcagctta aggttgctct gaatgaaggg gagcgtgccg aagaaataac
    45541 ccgcgtaggt aaaaatcagt acccacaaca gtgcgccgat cacgttataa gcggcgaaat
    45601 gacggtacga catgtggccc attcccgcaa caaacggcgc gaacgttctg acgatcggta
    45661 caaaacgggc gagaataatc gttttgccgc catgtctctc gtaaaactga tgagtcttat
    45721 caagataact gcgacgaaag attttcgaat ccgggttact gaacagtttg tcaccaaata
    45781 accgtccaat ggtatagttc actgcatcac cgacaatagc ggcaatcaac ataagcacca
    45841 ccatcatatg gacattgaga tcgttagttt ccagcgacgc caatgcacca gcaacaaaca
    45901 gcagcgaatc accaggcagg aatggcgtta ccaccagccc cgtttcgcag aacagaatca
    45961 aaaacagaat ggcataaacc cagacgccgt actccgcgac cagctctgcc agatgcacgt
    46021 caatgtgcag gataaaatcg atgaggaaat aaatcaggtc catatttgcc tatgccttgt
    46081 actcgtcatc tttcaggctg taactacgtt ggctacagcc tgaaatgttc cgagcattat
    46141 tctcgttagt ccgccagaaa tagcgggccc attggcggtt ttggaagatc ataccggtca
    46201 gggtaatcca ccgcgaccag atacagccct tccgcttttg ccgttgctgc cgccagcgtt
    46261 ctgtcctttg ccgccagcaa ctctgctatc cagctctccg gctggttgtg ggcaccgact
    46321 tccatcaggc tgccgacaat attcctgacc atatgatgta caaaggcatt cgctttgata
    46381 tctaccacca cataaggacc gtgacgcgtg acattaatat gcataacgtt acgccacggg
    46441 gttcgggact ggcactgcac cgcacggaac gaggtgaaat cattctcgcc cagcaagcat
    46501 tgcgcagccc gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt taccccttta
    46561 ctcagtaccg ccgggcgcag ccgatgattg tagatgatgt agcgataacg gcgagccgtg
    46621 gcgctaaatc gggcatgaaa atcatcaggt acagctttaa cccaacgcac ggcgatgtca
    46681 ccaggtaaat tcgcatttac gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt
    46741 tcgaaatgca ccacttgccc ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag
    46801 acggtgatgg gttcgttcgc cacctgggag agcgcctttt ccagcttctc ctgcacactg
    46861 cggacttcat tctgccgttg ccagccgtaa tacttactgc cgtcgtactc aatgcccagc
    46921 gcaattttat aaactggcag ttgttgctgg tcggacatta gtacagatac tcctgcacca
    46981 gtttctcggc gatttttact gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg
    47041 accagaactg gacttgctcc ggcataccgt agtcattacg cacgcagcca acagaaagat
    47101 gcggcgtacc cgaagcatcc cctacctgag tcgggaattc gttctcttca gagagcacaa
    47161 tatcttcgcc ctgagcaaac gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt
    47221 caaagttgac catctgggca tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa
    47281 tcatcagccc ttcgtcctgc aggattttgc gtacttcgtc aacgatacga cgttcttcac
    47341 gcacgctacc ttcgctatcc ggcaacaacg gcagcatatt gaacgccagt tgacgaccga
    47401 agaaatcttc ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc cccgctaacg
    47461 catcgaccgc ttttttgccc tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg
    47521 acaaaccgcc ctgatcgatt aacggtttca gtgccgccag cagctggctg gtcagactgt
    47581 ctggtacggc gatgacattc cggttgcggt aatcggtcag tacaaacggg tttacttccg
    47641 gcaccaccag cggtacgtcg ggttcgagag caaacagtcc gctgctgtcg atcaccaggc
    47701 aacctgagtt ggtcgcttct tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa
    47761 atgccagctg cgcctgcgtc cagtcgaatt cagcggcatc ctgcacggtg attgttttac
    47821 caccaaagcg cagttgttcg cctgcgcttt cgttacgtgc cagtgcataa atttccccaa
    47881 ccgggaactg acgttcagcc agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc
    47941 ccaggacggc aatgttccag ccttcagaca tggtggttta ctccagaaat agcaaagctc
    48001 cctgccaatg ttacagcaga gagcatgaag aagagattaa cgtgccggat gatgaacggc
    48061 gttaaaaccc agtttacaca gcaatgatgc cgcactggcg tcatcacaaa ttacatacag
    48121 agatgaccat tcacggcgct caagatagtt tttgcgcagt ttatcgaact cacccggtat
    48181 cccggcgact ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag
    48241 ccttttcagc gtcggttgat cgagcgggcc atgcagcgta atgcgaccaa actctggcgc
    48301 gggcagtaat gtatccagcg caacgtgctg ttcatgccca ataaacttgc tataggcttc
    48361 aaacacttgc gtagtaccgc gtgctttacc ttccagggta taacctgcga tatgcggcgt
    48421 gccgatatcc acttttgtca gcaactccac gttaagttcc ggttcgcctt cccagacatc
    48481 cagcactacg cttaacttct ggccttcatt caggcaggtc agcaacgcag tattatcgac
    48541 gactgcgcca cggcaggcgt taatcagaat cgctcccggc ttcaggctac ggatcagttt
    48601 ttcatccgcc agatgcagcg ttttgtacgg cccatctttg aagagtggcg tatggaaagt
    48661 cagaatatcc gcatgctgaa ctaattcatc cagcgagcgg aaatcaccct catccccacg
    48721 gtcagcgcga ggcggatcgc aaagcaatgt tttaatccct aacgcttcca gtcgcgcctg
    48781 caatcgacgt ccaacgtaac caacgcccac gatccccacc gtacggtcgt gcagtgaaaa
    48841 tccatcgcgt tcggcaagca tcagtagtga ggagaaaaca tattccacca ccgcaatcgc
    48901 attacagcca ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac
    48961 atggtcggtc cccgcagtgg cagtgccaac aaatttaatg ggttttcctg ccagcaaaga
    49021 ttcattcact ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac
    49081 ggggattgga cgcccgggaa ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc
    49141 ataaggcata ttttcatcaa caaggatttt cacgtttgtg ttacctgtat gagacgagag
    49201 ttaaccggac aagtgtgcca taatctcgcg gccaggcata cttgcgaaga tttcaggtat
    49261 aaggatacgt aatgatacaa cctatttccg gccctcctcc tgggcaacca ccaggtcagg
    49321 gagataacct gccgtctggc gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc
    49381 tggaaagctt aatgacgaaa gtgacctcac tgacgcaaca gcaaagagca gaactgtggg
    49441 cgggtatcag gcacgatatt ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc
    49501 ctgccgctga gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc
    49561 gccagctttt agcgcaatta ggggagtatt tacgtctggg gaataatcgt caggcggtca
    49621 cggattatat ccgtcataac tttggtcaga cgccgctgaa tcagctctca ccggagcaat
    49681 taaaaaccat tctcaccctg ttgcaggaag ggaagatggt tattccgcaa ccacagcagc
    49741 gcgaggcgac cgaccgtcct ttattaccgg cggagcacaa tgcgctaaaa cagctggtga
    49801 ccaaacttgc ggcggcaacg ggggaaccca gcaaacagat ctggcaatcg atgctggaac
    49861 tttccggggt gaaagatggc gagttaattc cagcgaaact gtttaaccat ctggtgacct
    49921 ggctacaggc gcgtcagacg ctaagccagc aaaatacgcc gacgctggaa tcactacaga
    49981 tgacgctaaa acaaccttta gatgccagtg aactggcggc gttatcggca tatatccagc
    50041 aaaaatatgg tctttctgcg caatcatcgc tttcttctgc ccaggccgag gatattctta
    50101 atcagcttta tcaacggcgg gttaaaggga ttgatccgcg tgttatgcaa ccgctgctta
    50161 atccttttcc accgatgatg gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga
    50221 tactgttagt cgcgattatc ctgatgctgg tctggctggt tcgttaaccc cgacgaaatg
    50281 acagtatcgt gacaataata cccagcaccg cagagatcgc cccggcaaga aataccgaag
    50341 agtaaccaaa cgtggtcgcc agcattcccg ccagcggccc ggagacgccg agggcgatat
    50401 cctgaaacgc ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa
    50461 ccacctccac gcccagcgca ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc
    50521 ccgctaatgc gacccatgca cctggggctt gccagagcag caacaagccc accgtttcta
    50581 caagcagaga gacaatcgcc actttcacgc cgccaaaacg gtccggcatc cagccaaaca
    50641 tgacgcgcat cacgacaaat gcgccgccaa acgcggtaag agtaaagccc gccatcgccc
    50701 atcctttgct ggcaaagtag agcgaaacga aagtcccgat aacagcaaaa ccaacgcctt
    50761 gtagtgccag acctaaccct ggtttccaga taagcccgac aacgctccac agcgatggac
    50821 gttctcccgc cagggccggt actttgcgca ctgtgccgtt acaggcccac gccagtacgg
    50881 gtaataccat tgtggtgatc gccagtgcgg caaaaccgta atggctatga atcaacaggc
    50941 caagcggagc accaacagcg agggcaccgt aaatcgccat tccgttccat gacatcactt
    51001 tgccagagtg ttttggccct acgatgccta gtccccaggt cagagcgcct gtcagtaact
    51061 ggctttcacc aaagccaaga atcaaacgcc cgacgaccaa cagagcaaat ttgaacggtg
    51121 cggagacagg caaaatcgcc gccagcagca acgcgccgcc agccagacca caagctaaca
    51181 ttccctgaag cgccgaacgt tttgcaccat attgatcggc cagtcgcccg gcgtaaccac
    51241 gcgtcagcac cgtagccaga aactgaatcc cgacggcaat gccgaccatg gtattgccat
    51301 agcccagttc atgatgaaca aacagcggga taaccggcaa cggcaaccct acggtcatgt
    51361 aggtgagaaa aaccgcaaaa gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt
    51421 cggtttggct tacagcagtc atgcattact ccagaatgca gcgcaaggcg aggagtatcc
    51481 ccgtctcatc tctctggttt cagggttacg gtgcgttggc aggatttaac gcgtacgtct
    51541 tttcagaagg aaatcgacaa agcgggaagt ttgcctggaa ctggcggcga ttgtcaatga
    51601 tgtgaaaaag ggaaccatca ggttcccttt tgcgttagtg ccggaggccg cattggcgcg
    51661 taacgtcgga tgcgacgctg gcgcgtctac tccgacctac tgcgaattaa tctttcagct
    51721 tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac ataacggtgg
    51781 tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc cgcagcctgc tcgtccagct
    51841 cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga gagtagatag
    51901 cttcctgtac gccagcagcg cccagagagt gaccggtcat ggctttggtt gcagaaatcg
    51961 ccgggctctt atcgccgaac acttcacgga tagctgccag ctctttcacg tcgccaaccg
    52021 gagtcgaagt accgtgggag ttcaggtaat cgattggggt atcaacgcca tgcatcgcca
    52081 tcttcatgca gcgtactgcg ccttcgccag acggagcaac catgtctgca ccatcagagg
    52141 ttgcgccgta gccaacgatt tcagcataga tgtgagcacc acgcgccagc gcgtgttcca
    52201 gctcttcaac cactaccata ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt
    52261 cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagatagc gcacccattg
    52321 cgtcgaattc gcaagccatt tcccagcaca gctcttcgcc gccgccagca aacacgatgt
    52381 cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg gaagtcgcac
    52441 acgcggagct gatggagtag ttaacgccat gaattttaaa cggggtggcg aggcaggcag
    52501 aaacgccgga tgccatcgct ttggtgacca catacgggcc aaccgctttc aggccgcgcg
    52561 ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg ggagccgccg ccggaacctg
    52621 caatcaggcc aacgcgcggg ttattctggt aagcttccgg agagaggccc gcatcagcga
    52681 ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag cgcacaactt
    52741 tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg ctacgcatgc
    52801 cggaatcctt cagctcctga gagaaagtga tccctgaacg tccttcacgc agagatgcca
    52861 ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta atcactgcac
    52921 gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca caatagcgta
    52981 cacttgtacg ccgaacaagt ccgatcagcc atttaataga gaaatttgcg cagccttaca
    53041 cacatcgcta agatcgagcc accgcctgta agacgagtaa cttacgtgaa acactactcc
    53101 atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg agattttgac
    53161 gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt tctgggaggc
    53221 aaccaattag aggtacgctt tcctgagcat ccacatcctc tgtttgtggt agcagagagc
    53281 ggcttcggca ccggattaaa cttcctgacg ctatggcagg catttgatca gtttcgcgaa
    53341 gcgcatccgc aagcgcaatt acaacgctta catttcatta gttttgagaa atttcccctc
    53401 acccgtgcgg atttagcctt agcgcatcaa cactggccgg aactggctcc gtgggcagaa
    53461 caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt gctcgatgca
    53521 ggccgcgtga cgctggattt atggtttggc gatattaacg aactgaccag ccaactggac
    53581 gattcgctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc agcgaaaaac
    53641 ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg tccgggcggc
    53701 acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga cgccggattc
    53761 acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa tgctttgcgg ggtgatggaa
    53821 cagacattac cgctcccctg ctccgcgccg tggtttaacc gcacgggcag cagcaaacgg
    53881 gaagcggcga ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc gctattacgg
    53941 cgcggctggc aggtaacgct ttattgcgcg gatgaggccc ccgcactggg tgcttccggc
    54001 aatcgccagg gggcgctgta tccgttatta agcaaacacg atgaggcgct aaaccgcttt
    54061 ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc aattacccgt taaatttgat
    54121 catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca taaaatcgca
    54181 cagatgttgt caatggattt acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt
    54241 gaacaaatta cgggcgttgc gacaaattgc agcggcatta cttatcctca aggtggttgg
    54301 ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag
    54361 atttattatc aatatcagtt acagaattta tcccgtaagg atgactgttg gttgttgaat
    54421 tttgcaggag atcagcaagc aacacacagc gtagtggtac tggcgaacgg gcatcaaatc
    54481 agccgtttca gccaaacgtc gactctcccg gtgtattcgg ttgccgggca ggtcagccat
    54541 attccgacaa cgccggaact ggcagagctg aagcaggtgc tgtgctatga cggttatctc
    54601 acgccacaaa atccggcgaa tcaacatcat tgtattggtg ccagttatca tcgcggcagc
    54661 gaagatacgg cgtacagtga ggacgatcag cagcagaatc gccagcggtt gattgattgt
    54721 ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt
    54781 gtgcgttgtg ccacccgcga tcatctgcca atggtaggca atgttcccga ttatgaggca
    54841 acactcgtgg aatatgcgtc gttggcggag cagaaagatg aggcggtaag cgcgccggtt
    54901 tttgacgatc tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg
    54961 tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga tgccagtacg
    55021 ctggcggcgt taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt
    55081 aaggcggggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc cttatccgac
    55141 ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc atggtgctcc
    55201 agacgccgca acttactgct gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac
    55261 cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg gctttttcca gactctggct
    55321 aacggtcgtg tgcaccgcct gagcggagtg gtcatcacca ctttccagtt ctgcgatggc
    55381 taatgtcagg tggccacgca aatacccact ggcaaacaac tcatcatcac tggcatggtc
    55441 aaccatatcg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca tcttctttcc
    55501 tcattgtaaa acgtggcgct ggctttagtt gagcgcttcc ggccacggga actgttccgc
    55561 cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaagcgtg ccgggcgttc
    55621 aggaatgccg tttgctaggt aatccatcac ctgcgcatgt acccgccgtt gaaacaccac
    55681 acgatccggt tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg gatatcccgc
    55741 cgccacacag aacaaccagt cgaacgcctg cggcttcact tcaacatctt caaactggct
    55801 ttgcgtttgg gcatcgcgtc cgtccgggca ataccagtag ccgaaatcaa ccagctcacg
    55861 gcgcgctttc ccggcaatac accagtgcga aatctcatga attgcgctgg cataaaagcc
    55921 atgagcaaag acgattcggt tatacggtac ttccgcatca gcaggaagat agatcggttc
    55981 gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca aagcagctat taaaaatttc
    56041 aatcaactgc tcatagtggt gtgtactgtt cattagttca tccccaacca gtggaggatc
    56101 tcctgtccgt ggctatcaaa aagtaatttg gcactcatca ccgctgagac aataacaatc
    56161 atcgggcgga tcagcttttg tcctttgctc aacaccagtc gtgaacccat gcgcgcgccg
    56221 aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc accgagaata
    56281 aacagcagta aaccgccgat gtttgacgtt gcgttgagta atttggcgtg cgccgtggct
    56341 ttggcgaggt tgaatccgca cagcgtaaca aaggccagtg cgtaaaacga tccggctgcc
    56401 ggaccaaaga atccgtcata aaaaccgacg cagccaccgg caattaacgc gaagggtaaa
    56461 ccgtacatcc gacgctggcg atcttcttca cccagctttg gcatcagcaa gaaatagagg
    56521 ccgatacaaa ttaccagaat gggcaaaatc tgccgtaaga catcagcctg aacgtattgc
    56581 accagcagtg cgccgctcat tgagccgaca aaggtcatgg cgatattgag tttctgatcg
    56641 cttaaactaa ccactttgcg gcgaataaag tagatagtgg cggaaataga gccgccgcag
    56701 gcttgcagtt tattggttgc cagcgcgtta gcgggagaca ttcccgcagc catcaatgcc
    56761 ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc cagcatggcg
    56821 acaaaaaaga gaactcccag caacagcggg gaaaccataa acaggctatt aaacgtttcc
    56881 attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct
    56941 tcttctcagg ctttgttgtt cccggttttg gaggtgcaaa ccagctttgc agttctgccc
    57001 cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca ctccagacta tcggcaggac
    57061 aacgtaatcg tacatgcata tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc
    57121 agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa
    57181 tgcgtgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc
    57241 acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca
    57301 agagctgcgc ggaggtccag cgagttttcg gcagttgcag aaagatatcg acatccagtc
    57361 cggtctggtg gctggcatga ccgccgttga aacgcccacc agcgggcatc cccatatcgc
    57421 caatcagcac cgtacccatg cccagattgc tcacctggct actcagacgc tggataaaca
    57481 tcaccagatc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc tgataatgtt
    57541 cggactgtat cggcagcgtg tcagcgccga cgatacagcc attagaaaaa ctgcctatcg
    57601 attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct gccaggctag
    57661 cgctactggc aagcagagcc agcagcgcaa tcgcggtttt gttcattttt taccagcgtg
    57721 gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga tccattaaaa
    57781 cgatcgccag catcgcttca gcgatcggca ctgcacggat cccgacacag ggatcgtgac
    57841 ggcctttggt gatcatctca acttcttcgc caaagcggtt aatagtgcgc cccggcacgg
    57901 taatgctgga ggtcggtttc agcgccatat gagcaatgat ttgctgcccg ctgctgatac
    57961 cgccgagaat gccgcccgca tggttgctct ggaaaccgtc tttggtgatt tcgtcgcggt
    58021 tctggctgcc gcgcagcgcc actacgtcaa aaccgtcgcc aatttccacg cctttcaccg
    58081 cgttgatgct catcagcgca tgggcgatgt cggcatccag gcggtcaaag accggctcgc
    58141 caagtccggc ggggacacca ctggcaacaa cggtgacttt cgcgccgatg gagtcgccct
    58201 cttttttcag cgcgcgcatc agttcatcta acgcgtcgat tttgtcgggg tccgggcaga
    58261 agaacggatt ttgttcaacc agcgaccagt ctttgatttc cagcggaatg tcgcccatct
    58321 gggtcaagca gccgcggatt tcaataccaa atttctcggc gagatatttt ttggcaatcg
    58381 cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg ccgcgataat
    58441 cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg gcctgggcgg aaaacgtctt
    58501 taattgcgct gtaatcctga gagcgctggt cagtgttttc gatcaacaag ccaatgctgg
    58561 tgccagtagt aacgccttca aaaacaccgg agagaatttt gacctgatcc ggctcgcggc
    58621 gctgggtggt atagcgcgat gtcccagggc gacgacggtc gaggtcatgt tgcaggtccg
    58681 cttccgtcag cggaatgcct ggcggaacac catcgacgat gcagccgagc gccagcccat
    58741 gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg
    58801 ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa atattcacgg
    58861 gccgccagca gctgctcttt ggtgagcata aacacaccat cgccgccgtt atcaaactcc
    58921 agccaggtga acggaacatc cggatattgt tccataagat gtaccatgct gttgccgact
    58981 tcacaaatca acacgccatc atcagcaagg taatctgccg cgtttccaag aatgcgacgt
    59041 gtcagtttaa ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg acggtattcg
    59101 tttggcaggt cggacatatc ttccgcgtcg acatacggcg ggttagtgac aatcagatcg
    59161 tattgcactt tcggcaaatc acggaacaga tcggaacgaa tcggaatgac gttgtggatc
    59221 agaccgtgtt cttcgatgtt ctgttcggca accgccagcg cgtcgggaga gatatctacc
    59281 gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa tggcgatgca gccgctaccg
    59341 gtacacatat ctaaaatatg ctgcggttgc ttgctgatga gtccggcaaa tttattgttg
    59401 atcagttcac caatcggcga gcgcggcacc agcacgcgtt catcgacgta aaattcatga
    59461 ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa tgcgctcgtt gacgcggcgg
    59521 atcacgcgtt caacaatacg gtgtttttcg ctggaggtca gacgcgcggt gcgcatatct
    59581 tccgggatat ccagcggcag gtagagcgat ggtaacacca gctgtacggc ttcatcccac
    59641 gggttatcgg tgccgtgacc gtaccagata tttgccgcgc tgaagcggct taccgaccag
    59701 cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa aattttatcc
    59761 acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca tgaagatgac
    59821 gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg atcgattgtg
    59881 ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg aaaaagaaaa
    59941 caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg actcgcaaga
    60001 ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa aatcagcgaa gtgccggtga
    60061 aacgcttgat ccaggagcag gctgatgcca gccattattt ctccgatgag tttcagccgt
    60121 tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat tttgaggcga
    60181 agaaactgcg ccgcggcgat tattcaccgg aattgttttt ggatttacac ggtctgacgc
    60241 agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc ctgtcgccgc gaacatgtgt
    60301 tttgtgcctg cgtgatgcat gggcacggga agcatatttt gaagcaacaa acaccgctgt
    60361 ggctggcaca acatccgcat gtgatggcat ttcaccaggc accgaaagag tacggcggtg
    60421 atgcggcttt gttggtgttg attgaagtgg aagagtggtt gccgccggag ttgccctgag
    60481 gagttgagcg tgttgtgcca ggagcgcgga atggcagggg cgaatgaccc tgccaggtga
    60541 gaaaaggtgc gctaatacat attcagtatg taagtgaatc ggcaggtaga atctatgggc
    60601 tgtcgagatg tcgtgtccac gcgtagcttc atatcatgca gttgcctgga cgtaatcggt
    60661 tgcggctcag gcgtcaccgt cactttggca ttatcacagc cggaagtcga gaaggtcatc
    60721 tgctgtgcaa taaaacggca gtaggcacca cgctcatcaa aattattccc gttacacagt
    60781 ttgccatttt ccatcaggaa agtgggcagt gtttgctcaa taacaccacc tgttgactgc
    60841 aacctgacga ctttgttatg tacaacataa ttttcattcc aggaaacatc atacggtgcc
    60901 aggggcaccg ccggacatga agaactcacc ttcgttgggc caatatagtc aggaaaatca
    60961 tcatcatacg ttttagcgaa gggatctcta ctcttaacaa acgacgacac aatccacgga
    61021 caaccatgta aattcccatc gtaataaaca aacatcttta attcattaca tgtccatccc
    61081 gttttggtct ggctatcata ctgctctaat ccaggacagg ctgacatgga taccctgctg
    61141 tccgaaattt tcgacataga tggttgagta gaactatcgc cagtccacga aactaaatcc
    61201 ggctgtgaat tgggcatgaa tgtagcctta aatatagcgt aattaactga atctggcgtc
    61261 gctataatca gatgaaactc aaactctgtc gcaggtgaac tggcaccagc tggcacatat
    61321 aactcaccaa ctttcgtcgc cgcctggccg catgtattga acattaacag caataaaaga
    61381 cataaaacat gacgaaggat tttcatttta aatatactca aaagttgctg tcaggctggc
    61441 ggtgaaatca ccgatggtta catcacgacc gcttttcgcc tggatccagg cccggaaatt
    61501 aatatcattg ttgccgttgg taagcgcaaa ggtcgccccg gtggtgttgt taatatccac
    61561 ccgcttgccg tcgagcgttt ccatccctat ccccacgccc tgcgcggtgg aggtgtcatc
    61621 gactgccaga aaacccggct gaccgctgtc ttcggtgccg ctcagcgtga ctttgacgtt
    61681 gtatcccgcc ggccctttgc agtctttcag cttaaacaca accggcagat gcgcggactg
    61741 cccagatacc atcaaatccc ggttgctgac ggtgggaaaa tggatttctg ccaggttggc
    61801 accatccacc accagcgtgc aggatttact cagcaggttg ccgtaaaaat gcaggttgtt
    61861 gtcgactgcc tgagctcctg tgctggcggt cagcagcagg cctgctaaca gcgcataacc
    61921 gggtgaataa atgtgtgctg ttttcattgg taatccaccc gtaaggttgc gtaagcagaa
    61981 aaggtcgtgt ccgtcagcac cgcatcagcc gctttgaccg gtaccgcctc cagctttggc
    62041 ttctggctaa aatcggtggc gttaatggaa agcggctcgt tgagcttaaa acgctgaccg
    62101 tcatgctgaa gctcaattcc gaagcccggt acatccgtct caatagcaga gtcgttgtag
    62161 gaggtggcag tccccgtcca ggagagtgtc atattccact cgggatcgcg gacatccgag
    62221 tcgcagctca gttcgtagtc cactttctga cgaccataat tgccatcaat gctgtcgata
    62281 atgagatcgc gaaattgcac ttcgatggtt tgcccgccgc tgatgctaca ggcgggcggc
    62341 gacatcagcg taccgtggaa tgtgatgtca tcttccgccg ccagcacagc cccgcaacca
    62401 gcgcacagta aaaggagcag gaattttttc attggtaatc caccaccatg gtcatcgtgg
    62461 cgttaaactc cgacggcgtc agttgcaccc cgctctgttt taccggcacc gctttcagct
    62521 gaggctgagt gttgacatta aaaggcgtcc agttattttc accaactgca aacagggagt
    62581 tgtcggcggc gttttctatc cgcaccccca ggccggcgat atccgtgctg agaaccgttt
    62641 ctccgttgat cacggcggta ttgcctttga gctgcatccg taagtcatcg ctgactttat
    62701 taccgcagtt gagggtgtat ttcgcatcct gttggtaatt ggtaccatcg atcctgttga
    62761 ttatcacatt gccaaatttc acagcgcttc ccttcacgga gcaaggcggc ggtccatcga
    62821 ccaggattcg cagcgtcaga tccacctgtt tgatatcact gccagcaaca gccaaaccgc
    62881 tgatgcccca tccggccagc accagcaacc ctgcgcccgc tacaaacttg tgactcataa
    62941 gtccctcccg gcatcagcct ttgcggctct gctcttcatc cacctggcag ctgttgccgg
    63001 tgcatatgaa gtacagcggc atccgcgcgc cgtagtcgtt gatgtaagtc agcaccggca
    63061 cgctgtccat cttcgcattc agcggtgcgg aggttttcgg ctccatcatc agcggtgtga
    63121 atcccgccgg cgggttgccg ccttttttag cgctggcgtt gctgatgatc acgtagtacg
    63181 gcgtcgggtt attgacggtg aagtcctggc cgttacggat gagcgtcacc ttatgctgcc
    63241 acggactttt catgctggtg ttctccagcg ccttcggtcg ccagaacagt ttgatacgcg
    63301 tctgcagcgc aatctgcagt gtgttcggtt tatttgattt cggcggaatt tcgcgcacgt
    63361 tgaaatagaa cacgctctcg cggtcggccg gcagtttgtt gatatccggc agcccctgca
    63421 ccttcacctg gccgttcatc atcgcatcga tgcgctgcac tggcggaagc accgcaagcg
    63481 gcgagctgat tttgttgccc tttgcatcct ctatccagct ctgcgccaga taaggcagtg
    63541 tgggatcgtt gttgcgcagc gtgacgctga tggacttgtc gctttcgtta aacaccagcc
    63601 gtgtcctgtc gggcgtcacc gaagcgtggc tggagaaact gaccgctgtc gccagcagcg
    63661 ccagtgccgg gacaacgatt tttgcagtgt tagtcagatt aaacatggat cattccttaa
    63721 tcacgttact ggtttactga aattgaagtc ggaggttccg ttggcgtcac ccgctgtgtc
    63781 tgttcctgaa tcgccggttt gaccgccggc gaagtgatag gtgctacatt gcctttatgc
    63841 tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata
    63901 tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt caccccagcc
    63961 agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact
    64021 tctgcgccaa acggaggatg gctgccgtct tgtaaacgca gcaccgccat cgctttttga
    64081 ccactgatga cggcaaattt gcggtagcca atcgcccctt cagttagcgt ggcttgtacc
    64141 accgactggg ttgcttcagc gttttcaggc aatctgttga ggtcgatata cgcctgattg
    64201 cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca
    64261 gcaccgttac cttctaccgg aacatcggca acgccatcgg catcaatcaa caagcgtgtc
    64321 ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc
    64381 tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc
    64441 tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg
    64501 ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg
    64561 ctgtccgtcc cactgccata gttaccgtta tagctaacgg tactgttgtc gccccacggc
    64621 atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca
    64681 gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg
    64741 ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc
    64801 gaaacacccg catcgcggaa gttctggtta taagtggcgg tgtacatctc tttgtcgttg
    64861 cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc
    64921 tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg
    64981 gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta
    65041 tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg
    65101 cgaccgacgc caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac
    65161 gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgaca
    65221 tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg acctgggcga
    65281 gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct
    65341 tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt
    65401 ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct
    65461 gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt
    65521 tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat atcggatctg
    65581 aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg ccaggcatag
    65641 tagcgactcc actcccattt tttttgagtt tcatcgccgc tgaattcatc gtcatcatca
    65701 ttactgcgag tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccccagg
    65761 ttaaccccga ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttca
    65821 tgccgggttt gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa
    65881 cgtgaaggcg gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg
    65941 actaatgcgg actggcttaa atcagcctta atttccatgc cttccagttg accggatttc
    66001 aggcatttag catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac
    66061 tgcgctacca gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca
    66121 taccagtaaa tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg
    66181 tattttccgg gctcaacata tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg
    66241 ccttttaatt ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta
    66301 taactccctg acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa
    66361 tggtcaggca tacacgtttc tatccgtttt tattcttgtt cggtcaaaat ccatttaaat
    66421 cccccatagc agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta
    66481 tttgcttcga aattacccag atctggcgca tcagctgcgc cgaccagcca ggctttaaag
    66541 ttcagtgtct gcttcgcttt gcctttattg gtagaagtgt ctttcactat tttctggtca
    66601 atacccatgc cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg
    66661 ttaaatgccg caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa
    66721 gaaacggtac cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc
    66781 agacgaatct ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg
    66841 atatcaccca tatcaacttc cagtttgtga tcgtccggaa cgatagaaca cactgagtct
    66901 tcaatggtgc cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag
    66961 gtgaaaacgc ccatcaccat tgccgcagca atagctgttt taacaaactt actcattatt
    67021 tgtccttaaa ctataaataa ttaaaaatat aaacattaca atacattgat tcagtcaata
    67081 cccaatgttt atacccagcc attgaatacg gcatgcgatc caaaaagaac aggtcgtaga
    67141 aaattgtatt ctttggtgtg gggccgcata gtgatgtttt cacccccctg atgcaattta
    67201 attttactaa agagtaaatg tagatttaat taatatattg acagaagaaa ggttatctta
    67261 aggacatcct taaaaaatac ggaataagta tttagaaaaa atagaaagta tatcaataat
    67321 gaatatcaac agcttaaaaa acaaacaatg aacatataat gcgattggca tttatccac
//
LOCUS       DAZFVO020000004        67091 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFVO020000004 DAZFVO020000000
VERSION     DAZFVO020000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 67091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.004"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            91..534
                     /gene="cmtB"
                     /locus_tag="ACHKNB_RS00980"
                     /old_locus_tag="pgaptmp_000196"
     CDS             91..534
                     /gene="cmtB"
                     /locus_tag="ACHKNB_RS00980"
                     /old_locus_tag="pgaptmp_000196"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HGU4193782.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKNB_RS00985"
                     /old_locus_tag="pgaptmp_000197"
     CDS             562..1950
                     /gene="cmtA"
                     /locus_tag="ACHKNB_RS00985"
                     /old_locus_tag="pgaptmp_000197"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HGU4193783.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKNB_RS00990"
                     /old_locus_tag="pgaptmp_000198"
     CDS             1965..3242
                     /gene="yggP"
                     /locus_tag="ACHKNB_RS00990"
                     /old_locus_tag="pgaptmp_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4193784.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKNB_RS00995"
                     /old_locus_tag="pgaptmp_000199"
     CDS             3239..4204
                     /gene="glpX"
                     /locus_tag="ACHKNB_RS00995"
                     /old_locus_tag="pgaptmp_000199"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4193785.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKNB_RS01000"
                     /old_locus_tag="pgaptmp_000200"
     CDS             4226..4735
                     /gene="fumE"
                     /locus_tag="ACHKNB_RS01000"
                     /old_locus_tag="pgaptmp_000200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HGU4193786.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4732..5445
                     /locus_tag="ACHKNB_RS01005"
                     /old_locus_tag="pgaptmp_000201"
     CDS             4732..5445
                     /locus_tag="ACHKNB_RS01005"
                     /old_locus_tag="pgaptmp_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HGU4193787.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5417..6094)
                     /locus_tag="ACHKNB_RS01010"
                     /old_locus_tag="pgaptmp_000202"
     CDS             complement(5417..6094)
                     /locus_tag="ACHKNB_RS01010"
                     /old_locus_tag="pgaptmp_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4193788.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6088..6765)
                     /locus_tag="ACHKNB_RS01015"
                     /old_locus_tag="pgaptmp_000203"
     CDS             complement(6088..6765)
                     /locus_tag="ACHKNB_RS01015"
                     /old_locus_tag="pgaptmp_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4193789.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6753..7460)
                     /locus_tag="ACHKNB_RS01020"
                     /old_locus_tag="pgaptmp_000204"
     CDS             complement(6753..7460)
                     /locus_tag="ACHKNB_RS01020"
                     /old_locus_tag="pgaptmp_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGU4193790.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIQNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7461..8039)
                     /locus_tag="ACHKNB_RS01025"
                     /old_locus_tag="pgaptmp_000205"
     CDS             complement(7461..8039)
                     /locus_tag="ACHKNB_RS01025"
                     /old_locus_tag="pgaptmp_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGU4193791.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTELFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8062..8493)
                     /locus_tag="ACHKNB_RS01030"
                     /old_locus_tag="pgaptmp_000206"
     CDS             complement(8062..8493)
                     /locus_tag="ACHKNB_RS01030"
                     /old_locus_tag="pgaptmp_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HGU4193792.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8865..9884
                     /gene="epd"
                     /locus_tag="ACHKNB_RS01035"
                     /old_locus_tag="pgaptmp_000207"
     CDS             8865..9884
                     /gene="epd"
                     /locus_tag="ACHKNB_RS01035"
                     /old_locus_tag="pgaptmp_000207"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HGU4193793.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9934..11097
                     /gene="pgk"
                     /locus_tag="ACHKNB_RS01040"
                     /old_locus_tag="pgaptmp_000208"
     CDS             9934..11097
                     /gene="pgk"
                     /locus_tag="ACHKNB_RS01040"
                     /old_locus_tag="pgaptmp_000208"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGU4193794.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11312..12391
                     /gene="fbaA"
                     /locus_tag="ACHKNB_RS01045"
                     /old_locus_tag="pgaptmp_000209"
     CDS             11312..12391
                     /gene="fbaA"
                     /locus_tag="ACHKNB_RS01045"
                     /old_locus_tag="pgaptmp_000209"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGU4193795.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12455..12553)
                     /locus_tag="ACHKNB_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /pseudo
     CDS             complement(12455..12553)
                     /locus_tag="ACHKNB_RS01050"
                     /old_locus_tag="pgaptmp_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12749..13609
                     /gene="mscS"
                     /locus_tag="ACHKNB_RS01055"
                     /old_locus_tag="pgaptmp_000211"
     CDS             12749..13609
                     /gene="mscS"
                     /locus_tag="ACHKNB_RS01055"
                     /old_locus_tag="pgaptmp_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4193796.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13748..14383
                     /gene="argO"
                     /locus_tag="ACHKNB_RS01060"
                     /old_locus_tag="pgaptmp_000212"
     CDS             13748..14383
                     /gene="argO"
                     /locus_tag="ACHKNB_RS01060"
                     /old_locus_tag="pgaptmp_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HGU4193797.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14476..15216
                     /gene="yggE"
                     /locus_tag="ACHKNB_RS01065"
                     /old_locus_tag="pgaptmp_000213"
     CDS             14476..15216
                     /gene="yggE"
                     /locus_tag="ACHKNB_RS01065"
                     /old_locus_tag="pgaptmp_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HGU4193798.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDRPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15383..16279
                     /gene="ygfI"
                     /locus_tag="ACHKNB_RS01070"
                     /old_locus_tag="pgaptmp_000214"
     CDS             15383..16279
                     /gene="ygfI"
                     /locus_tag="ACHKNB_RS01070"
                     /old_locus_tag="pgaptmp_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4193799.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16276..17754)
                     /gene="scpC"
                     /locus_tag="ACHKNB_RS01075"
                     /old_locus_tag="pgaptmp_000215"
     CDS             complement(16276..17754)
                     /gene="scpC"
                     /locus_tag="ACHKNB_RS01075"
                     /old_locus_tag="pgaptmp_000215"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HGU4193800.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17778..18563)
                     /gene="scpB"
                     /locus_tag="ACHKNB_RS01080"
                     /old_locus_tag="pgaptmp_000216"
     CDS             complement(17778..18563)
                     /gene="scpB"
                     /locus_tag="ACHKNB_RS01080"
                     /old_locus_tag="pgaptmp_000216"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HGU4193801.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18574..19569)
                     /gene="meaB"
                     /locus_tag="ACHKNB_RS01085"
                     /old_locus_tag="pgaptmp_000217"
     CDS             complement(18574..19569)
                     /gene="meaB"
                     /locus_tag="ACHKNB_RS01085"
                     /old_locus_tag="pgaptmp_000217"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HGU4193802.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19562..21706)
                     /gene="scpA"
                     /locus_tag="ACHKNB_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /pseudo
     CDS             complement(19562..21706)
                     /gene="scpA"
                     /locus_tag="ACHKNB_RS01090"
                     /old_locus_tag="pgaptmp_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21910..22803)
                     /gene="argP"
                     /locus_tag="ACHKNB_RS01095"
                     /old_locus_tag="pgaptmp_000219"
     CDS             complement(21910..22803)
                     /gene="argP"
                     /locus_tag="ACHKNB_RS01095"
                     /old_locus_tag="pgaptmp_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HGU4193803.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22945..23130
                     /locus_tag="ACHKNB_RS01100"
                     /old_locus_tag="pgaptmp_000220"
     CDS             22945..23130
                     /locus_tag="ACHKNB_RS01100"
                     /old_locus_tag="pgaptmp_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4193804.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23186..23845
                     /gene="rpiA"
                     /locus_tag="ACHKNB_RS01105"
                     /old_locus_tag="pgaptmp_000221"
     CDS             23186..23845
                     /gene="rpiA"
                     /locus_tag="ACHKNB_RS01105"
                     /old_locus_tag="pgaptmp_000221"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGU4193805.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24101..25333
                     /gene="serA"
                     /locus_tag="ACHKNB_RS01110"
                     /old_locus_tag="pgaptmp_000222"
     CDS             24101..25333
                     /gene="serA"
                     /locus_tag="ACHKNB_RS01110"
                     /old_locus_tag="pgaptmp_000222"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HGU4193806.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25562..25621
                     /gene="ibsC"
                     /locus_tag="ACHKNB_RS01115"
                     /old_locus_tag="pgaptmp_000223"
     CDS             25562..25621
                     /gene="ibsC"
                     /locus_tag="ACHKNB_RS01115"
                     /old_locus_tag="pgaptmp_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HGU4193807.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25954..26013
                     /locus_tag="ACHKNB_RS01120"
                     /old_locus_tag="pgaptmp_000224"
     CDS             25954..26013
                     /locus_tag="ACHKNB_RS01120"
                     /old_locus_tag="pgaptmp_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4193808.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26114..26716)
                     /gene="fau"
                     /locus_tag="ACHKNB_RS01125"
                     /old_locus_tag="pgaptmp_000225"
     CDS             complement(26114..26716)
                     /gene="fau"
                     /locus_tag="ACHKNB_RS01125"
                     /old_locus_tag="pgaptmp_000225"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGU4193809.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26737..26920)
                     /gene="ssrS"
                     /locus_tag="ACHKNB_RS01130"
                     /old_locus_tag="pgaptmp_000226"
     ncRNA           complement(26737..26920)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKNB_RS01130"
                     /old_locus_tag="pgaptmp_000226"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26962..27291)
                     /gene="zapA"
                     /locus_tag="ACHKNB_RS01135"
                     /old_locus_tag="pgaptmp_000227"
     CDS             complement(26962..27291)
                     /gene="zapA"
                     /locus_tag="ACHKNB_RS01135"
                     /old_locus_tag="pgaptmp_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGU4193810.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27459..28037
                     /gene="ygfB"
                     /locus_tag="ACHKNB_RS01140"
                     /old_locus_tag="pgaptmp_000228"
     CDS             27459..28037
                     /gene="ygfB"
                     /locus_tag="ACHKNB_RS01140"
                     /old_locus_tag="pgaptmp_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HGU4193811.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28063..29388
                     /gene="pepP"
                     /locus_tag="ACHKNB_RS01145"
                     /old_locus_tag="pgaptmp_000229"
     CDS             28063..29388
                     /gene="pepP"
                     /locus_tag="ACHKNB_RS01145"
                     /old_locus_tag="pgaptmp_000229"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HGU4193812.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYHGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29385..30563
                     /gene="ubiH"
                     /locus_tag="ACHKNB_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /gene_synonym="visB"
     CDS             29385..30563
                     /gene="ubiH"
                     /locus_tag="ACHKNB_RS01150"
                     /old_locus_tag="pgaptmp_000230"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HGU4193813.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30586..31788
                     /gene="ubiI"
                     /locus_tag="ACHKNB_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /gene_synonym="visC"
     CDS             30586..31788
                     /gene="ubiI"
                     /locus_tag="ACHKNB_RS01155"
                     /old_locus_tag="pgaptmp_000231"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HGU4193814.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHCLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31862..31945)
                     /locus_tag="ACHKNB_RS01160"
                     /old_locus_tag="pgaptmp_000232"
     CDS             complement(31862..31945)
                     /locus_tag="ACHKNB_RS01160"
                     /old_locus_tag="pgaptmp_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4193815.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            32235..33329
                     /gene="gcvT"
                     /locus_tag="ACHKNB_RS01165"
                     /old_locus_tag="pgaptmp_000233"
     CDS             32235..33329
                     /gene="gcvT"
                     /locus_tag="ACHKNB_RS01165"
                     /old_locus_tag="pgaptmp_000233"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HGU4193816.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33353..33742
                     /gene="gcvH"
                     /locus_tag="ACHKNB_RS01170"
                     /old_locus_tag="pgaptmp_000234"
     CDS             33353..33742
                     /gene="gcvH"
                     /locus_tag="ACHKNB_RS01170"
                     /old_locus_tag="pgaptmp_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HGU4193817.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33861..36734
                     /gene="gcvP"
                     /locus_tag="ACHKNB_RS01175"
                     /old_locus_tag="pgaptmp_000235"
     CDS             33861..36734
                     /gene="gcvP"
                     /locus_tag="ACHKNB_RS01175"
                     /old_locus_tag="pgaptmp_000235"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HGU4193818.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36995..37738
                     /gene="ygfF"
                     /locus_tag="ACHKNB_RS01180"
                     /old_locus_tag="pgaptmp_000236"
     CDS             36995..37738
                     /gene="ygfF"
                     /locus_tag="ACHKNB_RS01180"
                     /old_locus_tag="pgaptmp_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4193819.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNFSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37795..39228)
                     /gene="bglA"
                     /locus_tag="ACHKNB_RS01185"
                     /old_locus_tag="pgaptmp_000237"
     CDS             complement(37795..39228)
                     /gene="bglA"
                     /locus_tag="ACHKNB_RS01185"
                     /old_locus_tag="pgaptmp_000237"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HGU4193820.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKEPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39273..39584
                     /gene="yqfB"
                     /locus_tag="ACHKNB_RS01190"
                     /old_locus_tag="pgaptmp_000238"
     CDS             39273..39584
                     /gene="yqfB"
                     /locus_tag="ACHKNB_RS01190"
                     /old_locus_tag="pgaptmp_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HGU4193821.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39748..40407
                     /gene="yqfA"
                     /locus_tag="ACHKNB_RS01195"
                     /old_locus_tag="pgaptmp_000239"
     CDS             39748..40407
                     /gene="yqfA"
                     /locus_tag="ACHKNB_RS01195"
                     /old_locus_tag="pgaptmp_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGU4193822.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40603..41583)
                     /gene="ygfZ"
                     /locus_tag="ACHKNB_RS01200"
                     /old_locus_tag="pgaptmp_000240"
     CDS             complement(40603..41583)
                     /gene="ygfZ"
                     /locus_tag="ACHKNB_RS01200"
                     /old_locus_tag="pgaptmp_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HGU4193823.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLGLKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41826..42092
                     /gene="sdhE"
                     /locus_tag="ACHKNB_RS01205"
                     /old_locus_tag="pgaptmp_000241"
     CDS             41826..42092
                     /gene="sdhE"
                     /locus_tag="ACHKNB_RS01205"
                     /old_locus_tag="pgaptmp_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HGU4193824.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42073..42480
                     /gene="ygfX"
                     /locus_tag="ACHKNB_RS01210"
                     /old_locus_tag="pgaptmp_000242"
     CDS             42073..42480
                     /gene="ygfX"
                     /locus_tag="ACHKNB_RS01210"
                     /old_locus_tag="pgaptmp_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HGU4193825.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42520..43041)
                     /gene="fldB"
                     /locus_tag="ACHKNB_RS01215"
                     /old_locus_tag="pgaptmp_000243"
     CDS             complement(42520..43041)
                     /gene="fldB"
                     /locus_tag="ACHKNB_RS01215"
                     /old_locus_tag="pgaptmp_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HGU4193826.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43153..44049
                     /gene="xerD"
                     /locus_tag="ACHKNB_RS01220"
                     /old_locus_tag="pgaptmp_000244"
     CDS             43153..44049
                     /gene="xerD"
                     /locus_tag="ACHKNB_RS01220"
                     /old_locus_tag="pgaptmp_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGU4193827.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44074..44784
                     /gene="dsbC"
                     /locus_tag="ACHKNB_RS01225"
                     /old_locus_tag="pgaptmp_000245"
     CDS             44074..44784
                     /gene="dsbC"
                     /locus_tag="ACHKNB_RS01225"
                     /old_locus_tag="pgaptmp_000245"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HGU4193828.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44790..46523
                     /gene="recJ"
                     /locus_tag="ACHKNB_RS01230"
                     /old_locus_tag="pgaptmp_000246"
     CDS             44790..46523
                     /gene="recJ"
                     /locus_tag="ACHKNB_RS01230"
                     /old_locus_tag="pgaptmp_000246"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGU4193829.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46614..47712
                     /gene="prfB"
                     /locus_tag="ACHKNB_RS01235"
                     /old_locus_tag="pgaptmp_000247"
     CDS             join(46614..46688,46690..47712)
                     /gene="prfB"
                     /locus_tag="ACHKNB_RS01235"
                     /old_locus_tag="pgaptmp_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGU4193830.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47722..49239
                     /gene="lysS"
                     /locus_tag="ACHKNB_RS01240"
                     /old_locus_tag="pgaptmp_000248"
     CDS             47722..49239
                     /gene="lysS"
                     /locus_tag="ACHKNB_RS01240"
                     /old_locus_tag="pgaptmp_000248"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4193831.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49282..49830)
                     /gene="idi"
                     /locus_tag="ACHKNB_RS01245"
                     /old_locus_tag="pgaptmp_000249"
     CDS             complement(49282..49830)
                     /gene="idi"
                     /locus_tag="ACHKNB_RS01245"
                     /old_locus_tag="pgaptmp_000249"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGU4193832.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            49885..49956
                     /locus_tag="ACHKNB_RS01250"
                     /old_locus_tag="pgaptmp_000250"
     CDS             49885..49956
                     /locus_tag="ACHKNB_RS01250"
                     /old_locus_tag="pgaptmp_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4193833.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49953..50078)
                     /gene="yqfG"
                     /locus_tag="ACHKNB_RS01255"
                     /old_locus_tag="pgaptmp_000251"
     CDS             complement(49953..50078)
                     /gene="yqfG"
                     /locus_tag="ACHKNB_RS01255"
                     /old_locus_tag="pgaptmp_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HGU4193834.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50080..51528)
                     /gene="uacT"
                     /locus_tag="ACHKNB_RS01260"
                     /old_locus_tag="pgaptmp_000252"
     CDS             complement(50080..51528)
                     /gene="uacT"
                     /locus_tag="ACHKNB_RS01260"
                     /old_locus_tag="pgaptmp_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HGU4193835.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51964..53883
                     /gene="ygfT"
                     /locus_tag="ACHKNB_RS01265"
                     /old_locus_tag="pgaptmp_000253"
     CDS             51964..53883
                     /gene="ygfT"
                     /locus_tag="ACHKNB_RS01265"
                     /old_locus_tag="pgaptmp_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HGU4193836.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53883..54371
                     /gene="ygfS"
                     /locus_tag="ACHKNB_RS01270"
                     /old_locus_tag="pgaptmp_000254"
     CDS             53883..54371
                     /gene="ygfS"
                     /locus_tag="ACHKNB_RS01270"
                     /old_locus_tag="pgaptmp_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4193837.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54407..55774)
                     /gene="ghxQ"
                     /locus_tag="ACHKNB_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /gene_synonym="ygfQ"
     CDS             complement(54407..55774)
                     /gene="ghxQ"
                     /locus_tag="ACHKNB_RS01275"
                     /old_locus_tag="pgaptmp_000255"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HGU4193838.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55810..57126)
                     /gene="guaD"
                     /locus_tag="ACHKNB_RS01280"
                     /old_locus_tag="pgaptmp_000256"
     CDS             complement(55810..57126)
                     /gene="guaD"
                     /locus_tag="ACHKNB_RS01280"
                     /old_locus_tag="pgaptmp_000256"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HGU4193839.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57144..58544)
                     /gene="xanQ"
                     /locus_tag="ACHKNB_RS01285"
                     /old_locus_tag="pgaptmp_000257"
     CDS             complement(57144..58544)
                     /gene="xanQ"
                     /locus_tag="ACHKNB_RS01285"
                     /old_locus_tag="pgaptmp_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HGU4193840.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58709..61579)
                     /gene="xdhD"
                     /locus_tag="ACHKNB_RS01290"
                     /old_locus_tag="pgaptmp_000258"
     CDS             complement(58709..61579)
                     /gene="xdhD"
                     /locus_tag="ACHKNB_RS01290"
                     /old_locus_tag="pgaptmp_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HGU4193841.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61576..62355)
                     /gene="ygfM"
                     /locus_tag="ACHKNB_RS01295"
                     /old_locus_tag="pgaptmp_000259"
     CDS             complement(61576..62355)
                     /gene="ygfM"
                     /locus_tag="ACHKNB_RS01295"
                     /old_locus_tag="pgaptmp_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HGU4193842.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62406..63734)
                     /gene="ssnA"
                     /locus_tag="ACHKNB_RS01300"
                     /old_locus_tag="pgaptmp_000260"
     CDS             complement(62406..63734)
                     /gene="ssnA"
                     /locus_tag="ACHKNB_RS01300"
                     /old_locus_tag="pgaptmp_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HGU4193843.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63737..66835)
                     /gene="ygfK"
                     /locus_tag="ACHKNB_RS01305"
                     /old_locus_tag="pgaptmp_000261"
     CDS             complement(63737..66835)
                     /gene="ygfK"
                     /locus_tag="ACHKNB_RS01305"
                     /old_locus_tag="pgaptmp_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HGU4193844.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT        16244 a        16832 c        17903 g        16112 t
ORIGIN      
        1 ggtttccctg ctcaattgct ttcattaaac cgcacgagta ttttattttt accgccggac
       61 ggtaccctaa aaataaaata aggataattt atgcggctta gtgattattt tccagaatca
      121 tcaatctcag ttatacattc agcaaaagat tggcaggaag ctatcgattt ctcgatggta
      181 tcattgctgg ataaaaacta tatcagcgag aattacattc aggcaattaa agattccacc
      241 attaacaatg gcccttatta tattctcgca ccaggcgtgg caatgcctca tgcgcgaccg
      301 gaatgtgggg cgcttaaaac cgggatgtcg ttgacattac ttgaacaagg tgtttatttt
      361 ccggggaatg acgaaccaat taaattactc atcggactct ctgctgccga tgccgattcg
      421 cacattggcg ctattcaggc gttaagtgaa ttactgtgcg aagaagaaat actcgaacaa
      481 ctcttaacag catcatcaga aaaacaatta gcggacatta tcagccgcgg ataattactc
      541 tctcctctac aggataatat tatggaaaac aagtctgctc gtgcaaaggt ccaggctttt
      601 gggggctttt tgactgcaat ggtcatcccc aatattggtg cttttattgc ctggggtttt
      661 attactgcgt tatttattcc caccggttgg ctgcctaacg aacatttcgc caaaattgtc
      721 ggcccgatga ttacctattt attgcccgtg atgattggtt ctacaggtgg tcatctggtc
      781 ggcggtaaac gcggggcggt catgggcgga ataggtacta ttggtgtgat cgttggcgca
      841 gagatcccga tgttccttgg ctcaatgatc atggggccgc tcggtgggtt ggtcataaaa
      901 tatgtcgata aggcactgga aaaacgcata cctgccggtt ttgagatggt tatcaataac
      961 ttctcattag gtatcgcggg gatgctcctt tgtctgctgg gttttgaagt gatcggcccg
     1021 gcggtgttaa ttgccaatac tttcgtcaaa gagtgtatcg aggcgctggt acatgcgggt
     1081 tatctgcctc tgctgtcagt catcaatgaa ccggcgaaag tgcttttcct taataatgcg
     1141 atcgatcagg gcgtctatta tccgctggga atgcaacagg cttcggttaa cggtaaatcc
     1201 atcttcttta tggtggcctc taacccaggt ccgggcctgg ggctgctgct ggcgtttacc
     1261 ttgtttggta aagggatgag taaacgttct gcgcccgggg cgatgattat tcacttcctc
     1321 ggtgggatcc acgaactgta tttcccatat gtgctgatga agccgctgac tattattgcc
     1381 atgattgcgg gcggtatgtc tggtacctgg atgtttaact tactggacgg tggtctggtg
     1441 gctggcccaa gtccggggtc tatctttgct tacctggcac tgacgccgaa aggctcgttc
     1501 ctggcgacaa ttgccggtgt tacggtaggt accctggtgt cctttgctat tacttcgctg
     1561 atactgaaga tggaaaaaac ggtggaaacg gagagcgaag atgagtttgc tcagtcagcc
     1621 aatgcggtta aggcgatgaa acaagagggt gcattctcgt taagcagggt taagcgtatc
     1681 gcctttgttt gcgatgcggg gatgggctcc agtgcgatgg gcgcgaccac cttccgtaaa
     1741 cgcctggaaa aagcggggct ggcaatagaa gtaaaacatt acgcgataga aaacgtgcct
     1801 gcggatgccg atatcgtcgt tactcatgcc agtctggaag ggcgcgtgaa acgtgttacg
     1861 gataaaccgc tgatattgat taataactat attggcgatc caaaactcga cactttattt
     1921 aatcaattaa ccgccgaaca taaacactga ttacagaggt aaaaatgaaa accaaagttg
     1981 ctgctattta tggcaagcgg gatgtccgtc tgcgcgaatt tgaactgcca gaaattaccg
     2041 ataatgaact gttagtgagt gtaatttctg acagcgtctg tttatcgacc tggaaagcgg
     2101 cgttactcgg tagtgaacat aaacgcgtac ccgacgattt agaaaatcat ccggtcatta
     2161 ccgggcatga atgtgccggg gttattgtcg aagtgggtaa aaatctcact ggcaaatata
     2221 aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt accaagcgga tattcagcag
     2281 gctacagcta cgaatatttt ggcggtaacg ccacctatat gattattccc gaaatcgcca
     2341 ttaatttggg ctgcgtatta ccgtatcacg gctcttattt tgctgcggcg tcgctggcag
     2401 agcctatgtg ctgcattatt ggtgcttatc atgccaatta tcacaccacg caatatgttt
     2461 atgagcatcg catgggcgtc aaacctggcg gcaatattgc actgctggcg tgtgcgggtc
     2521 cgatgggcat tggcgctatc gattacgcca ttaacggtgg catacaaccg tcacgggtgg
     2581 tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca gaagctgctg ccggtggatc
     2641 tggcggccag taaaggcatt gagctggtgt atgtgaatac caaagggatg agcgatccgg
     2701 tccagacgct gcgggcgctg acaggagatg tcgggttcga tgacattttt gtttatgcgg
     2761 cggtgcctgc tgtcgttgag atggctgacg aattactggc ggaagatggc tgtctgaact
     2821 tctttgccgg gccgacggat aaaaacttca aagtgccgtt taatttctac aacgtccatt
     2881 acaacagcac gcacgtagtc ggtacatccg gcggttcaac ggacgacatg aaagaggcga
     2941 ttgcgttaag cgccacgggg cagttgcagc cgtcctttat ggtcacgcat atcggtgggc
     3001 tggatgcggt gccagatacc gtgctcaatc tgccggatat ccctggcggt aaaaaactca
     3061 tttataacgg cgtgaccatg ccgctcactg ccattgccga ttttgccgaa aaaggcaaaa
     3121 ccgatccgct gtttaaagag ttggcgcggc tggttgagga aacgcacggc atctggaatg
     3181 aacaggccga gaaatatctg ctggcacaat ttggcgttga tatcggggag gccgcgcaat
     3241 gatgtccctg gcgtggccat tatttcgcgt tacggaacag gcagcgttgg ctgcctggcc
     3301 gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg gcggtcaccg cgatgcgcca
     3361 ggcattaaac gacgtcgctt ttcgtggacg agtggttatc ggcgaaggag aaattgacca
     3421 tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc gatgggccag aagtcgatat
     3481 cgcggttgac ccgattgaag gcacgcggat ggtagcgatg gggcagagca atgcgctggc
     3541 ggtcatggct ttcgcccctc gagatagcct gttacatgcg cccgatatgt atatgaaaaa
     3601 gctggttgtt aatcgactgg ctgccggggc gattgatctg tctttgcctc tggcggacaa
     3661 cttgcgtaac gtggcgcggg cgttaggtaa accgctggac aagctgcgca tggttacgct
     3721 ggataaaccg cgcctgagtg ccgcgattga agaagcaaca cagctgggcg tgaaggtttt
     3781 cgctctgccg gatggcgatg tcgccgccag cgtgctgacc tgctggcagg ataatcctta
     3841 cgacgtgatg tacaccatcg gcggtgcgcc agaaggcgtg atttctgcct gtgccgttaa
     3901 agcattaggt ggggatatgc aggccgagct gattgacttt tgtcaggcta aaggggatta
     3961 cacggaaaat cggcagattg cagagcagga gcgcaagcgt tgtaaggcaa tgggcgttga
     4021 cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg aacgatatcc tctttagcgc
     4081 tacgggcgtg acgggcggcg agttggtgaa cggtatccaa cagacggcga atggggtgcg
     4141 gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt aatataatag actccctgca
     4201 ttgatggtga tctatcggaa gaccaatggc gacgctgaca gaagatgatg tgcttgagca
     4261 actggatgca caggacaatt tattttcatt tatgaaaact gcgcattcta ttttgctcca
     4321 ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac gatgaagaga tcgtcgaata
     4381 tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac gatattgatg ttgcgctgcg
     4441 tttgatttat gcgctgggga aaatggataa atggctgtat gccgatatca cgcacttttc
     4501 ccagtactgg cattacctga acgaacagga tgaaacgccc ggatttgccg atgacatgac
     4561 ctgggatttt atcagcaacg tcaatagcat aacctgcaat gcgacgctct atgacgcgtt
     4621 aaaagcgatg aagttcgccg atttcgccgt ctggtcagag gcgcgtttta gcggaatggt
     4681 caaaacggcg ctgacgctgg cagtaacgac caccttaaag gaattaacgc cgtgaaaatt
     4741 gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc atgatgaaga gatagaacgc
     4801 gtgcataaac cgttgctgcg tatgctggcg gccttacaga cggtaagtcc gcagcgacgt
     4861 acggtggttt ttctttgcgc tccgccgggg acgggtaaat ctaccttaac taccttctgg
     4921 gaatatctcg cgcaacaaga ccctgaatta cccgctatcc agacgctccc gatggatggt
     4981 tttcaccatt acaatagctg gctggatgcg catcaattgc gccccttcaa aggcgcacca
     5041 gagacatttg acgttgcgaa actggcggaa aatctgtgcc aggtagtgga aggggattgt
     5101 acgtggccgc agtatgatcg acaaaagcat gatcctgttg aagatgcgtt gcacgttacc
     5161 gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg acgacgagaa gtggtgccag
     5221 cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc cagctactgc gttgcgggaa
     5281 cgactggtag ggcgcaagct ggcgggaggg gtatcgctgg cagacgcaga agctttttat
     5341 gaccgtaccg acgggccgaa tgttcgccgg gggctggaag agagtctgcc tgcaaatttg
     5401 accttaatga tgaccgctac gggtgaatat cgtctggtgg attaacgttc ctgattagac
     5461 catcttccag tcgcaccaca cgagagaaat gacgccgggt aaatgcggcg tcatgactga
     5521 ttgctacgac gctcgtgcct cgctggccgc acttctccag ccagctttca aacacgctca
     5581 accaattttc gtcaaaatcc cgactcggct cgtccagcag caatagtggc ggagagagtg
     5641 cttccaggca ggcgacggcg accatccgtc gctgcgcact atgcagatcc aacgggtgcg
     5701 agttggcagt gtctgctaac tgacaacatt gcagggcagc atgggtacgt tgagtgattt
     5761 catccgccgg gcatttttgc agtttcaaac caaatgcgat ctcgtcagcg acggtgctgt
     5821 gaaaaagttg gttttccgct tcctgaaaca gtacgccaac ctttgccgcg cgctggcggt
     5881 ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt accggctgtg ggcgtaagaa
     5941 gacctgccat tactcgtaac agcgtcgatt ttcccgcgcc attgtcgccg gtcagcgcca
     6001 accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag acaatccgtt gccgcgccag
     6061 gccagcgata agatatctta tttaacgtta acatagtggc tggagcgctc cgttttgcaa
     6121 ttgccacgct tgctggcagt agttcaggaa aggtgtgcga tgacgttcaa aaagaataat
     6181 cagcgaacca cgttcaaacg cccagtgttg taagcgctgc aacagcattt cgctggcctg
     6241 cggagtcagg cggctaaagg cttcatcaag aattaagagc tttggctgca tcgcgatggc
     6301 gcaggcgatg accacgcgct gtgtctctcc accggagagt gttgccgggt gacgatggcg
     6361 caacggctgg cactcggtca gagtcagggc ggcatcaatc cgcgccataa tctctgcttc
     6421 agcaagacac agattttctg gcccaaaggc aacttcttct tccacgctaa aggtacagcc
     6481 ggagagctgc aaatagggcg attgctggac gagctggatg gtggcggatt gttcattaag
     6541 cgggagatgc ccaatgggcg taccgaggag cgtccccgtg cctgttatct ctcctggcag
     6601 aaagtccgga taccagcccg ccatcagctg tgccagcgta cttttgccac tgccgttgtc
     6661 accaaagatg gcgaccatcc ccggtttgac atagtgaaag tcataacaga acggaggatg
     6721 cgttgagtgt gtcgggcaat aacggaactg ttctaacgta accatatcca cgctccgaat
     6781 tctgtcagca tcagcaagat catggtgtaa cgcgctacgc gttgtagggt gctatcggct
     6841 ggtgcccaca acgttgttcg tcggttttgg atccgaaatg cccgcatatc cagcgccgca
     6901 ccacggacag caagattgtt tagcgcattg tgtgttagcg gaataattaa cgcgggcatc
     6961 gcacgtaaac gctgatacca gccctcatcc agcggcacgc cgcgggcacg ctgcgcttca
     7021 tgaataatcg ctaattgccg ttttaactgc tcaacgacca ataacggccc ggcaaacaga
     7081 taagcgacgc ctggcggcag gcgagaagca aacagagcgc ggataaaaca ttgcacgggg
     7141 acgtactgca tccacaactg tgaggttgaa acaatcgcca gaatccgcaa ccagagcgta
     7201 atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc ttagccattc tggtagccag
     7261 ccaccgtgta ccagccacag cccggctccc agcgagaaca tcagccagac gacatatttt
     7321 gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa aggtcgccgc gctatagatc
     7381 ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg ccgccaatgc ccacagtgtt
     7441 aatgaggtaa atggatgcat ttagcgcacg gcagccattg ccggaaaatg gcgcgtggtg
     7501 cgaatcggca gctggcgcag taaaagccag acaatcaccg ctgtaagaat tttatcgacc
     7561 aggttcgcgc ctatcaccgt aatcgccacc gactccacca ggttttgccc catcgagtgc
     7621 atccaggcaa cgaacaaatc ggctccgcta ccggtgacac cgccgaacag ctcagtacgc
     7681 agcggtacag cgacgaccgt taccgcaagc gtaataatca cgccgctgac aacgactttt
     7741 ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg cgaccaggcc gatgaccatc
     7801 gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca atccccagag taaattggtc
     7861 agtaagccgg tcagcattcc gatcaccgga ccaagcagta ccgcgctaat aagtgtgccg
     7921 atggagtcga gaaatatagg taatttgacc atgctggcca gttgcccgcc gatcatattg
     7981 atggcgatag atataacgat caacaccagt gcctgactgg aaaagtggct acgcgccata
     8041 agaaaacctc tttgaaagta gttatttaac gggggaaata tgcaactcgt cgtaaccaga
     8101 aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg ctaccgatca ctagttcaag
     8161 cgtggtccgc acggtacagc cgggcatcat atgaccgcca agcatcgtgc cgtgtggatc
     8221 ggaaacgcag agatgaagat gctcaccact ctgttccagc gtgccgttta gtgagatcac
     8281 ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg gcatagcgca aggcgacgtc
     8341 agtcaggcta ccggtacagc ctgcaatcca ggcggcatga agttgatgct gctgtgcaaa
     8401 agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg cgcagtgcgt aaaagcgtgc
     8461 ggaggaagaa tatgggtagg gcaatgtcat cattggagta ctccgaaact cggacgtatt
     8521 gcggataata tcgacgctgc atcagtcata ttctgatata agtcagggtt tgtgacggca
     8581 caacgaaaag attttgctac gcttattcgt gattctgcaa agtggaataa attccacagg
     8641 ggcggggctg aaaacgtgac gtaagtcaga taattgcttt ctttgactgg acaatcattc
     8701 cttttattcc acgtttcgct tatcctagct gaagcgtttc agtcgattaa atgttcgaca
     8761 attaaccaat cagtcgcagt ttgcgacagg taaggtttcc ccggacgatt tgctggatta
     8821 ctctgtctgg caaatttgct cagggaaaac cttgcaggag atctatgacc gtacgcgtag
     8881 cgataaatgg cttcggtcgc atcgggcgta atgtggttcg tgctttgtat gaatcaggac
     8941 gccgggcgga aattaccgtg gtggcaatca acgaactggc ggatgctgcg ggcatggcgc
     9001 atttgttgaa atatgacacc agccatggcc gttttgcatg ggaagtacga caggaacgcg
     9061 atcaactttt tgttggtgat gacgccatcc gcgtattgca tgaacgttca ctgcaatcgc
     9121 tcccctggcg tgaacttggc gttgatgtag tcctcgactg caccggcgta tatggctccc
     9181 gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa agtgctcttt tcacatcctg
     9241 gcagtaacga tctcgacgcg accgttgttt acggcgtcaa tcaggatcaa cttcgtgcgg
     9301 aacaccgcat cgtttctaac gcttcctgta ccacgaattg cataattccc gtcatcaaat
     9361 tgttagatga tgcgtacggt attgagtccg gcacagtgac cacaattcac tccgccatgc
     9421 acgatcaaca ggttattgat gcataccatc ctgacctgcg tcgcacccgg gcagccagcc
     9481 agtcgatcat tccggtcgat actaaactgg ccgccggtat cacacgattt tttccgcaat
     9541 ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac cataaatgtg acggcaatcg
     9601 atttaagcgt gacggtgaag aaacctgtaa aagccaatga agtcaacctg ttgctgcaaa
     9661 aagcagcaca aggtgcattt catggtatag ttgactatac ggaattgccg ttggtctctg
     9721 tagattttaa ccacgatccg cacagtgcca ttgtcgatgg cacccaaacc cgggtcagtg
     9781 gcgcacacct gatcaaaacg ttggtctggt gcgataacga atggggcttt gctaaccgaa
     9841 tgctcgacac gacgttagct atggctactg ttgctttcag gtaagacgca agcagcgtct
     9901 gcaaaacttt tagaatcaac gagaggattc accatgtctg taattaagat gaccgatctg
     9961 gatcttgctg ggaaacgtgt atttatccgt gcggatctga acgtaccagt aaaagacggg
    10021 aaagtaacca gcgacgcgcg tatccgtgct tctctgccga ccatcgaact ggccctgaaa
    10081 caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc ctaccgaagg cgagtacaac
    10141 gaagaattct ctctgctgcc ggttgttaac tacctgaaag acaaactgtc taacccggtt
    10201 cgtctggtta aagattacct cgacggcgtt gacgttgctg aaggtgaact ggttgttctg
    10261 gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg aaaccctgtc caaaaaatac
    10321 gctgcactgt gtgacgtgtt cgtaatggac gcattcggta ctgctcaccg cgcgcaggct
    10381 tctactcacg gtatcggtaa attcgctgac gttgcgtgcg caggcccgct gctggcagct
    10441 gaactggacg cgctgggtaa agcactgaaa gaacctgctc gcccgatggt ggctatcgtt
    10501 ggtggttcta aagtatctac caaactgacc gttctggact ccctgtctaa aatcgctgac
    10561 cagctgattg ttggtggtgg tatcgctaac acctttatcg cggcacaagg ccacgatgtg
    10621 ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta aacgtctgct gaccacctgc
    10681 aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt tctctgaaac tgcaccggct
    10741 accctgaaat ctgttaacga tgtgaaagct gacgagcaga tcctggatat cggtgatgct
    10801 tccgctcagg aactggctga aatcctgaag aatgcgaaaa ccattctgtg gaacggtccg
    10861 gttggcgtgt tcgaattccc gaacttccgc aaaggtactg aaatcgtggc taacgctatc
    10921 gcagacagcg aagcgttctc catcgctggc ggcggcgaca ctctggcagc aatcgacctg
    10981 ttcggcattg ctgacaaaat ctcctacatc tccactggcg gcggcgcatt cctcgaattc
    11041 gtggaaggta aagtactgcc tgcagtagcg atgctcgaag agcgcgctaa gaagtaaaaa
    11101 atcacagggc agggaaacct gcccttgttt cagcgcgctt ttagagcacg cattttttca
    11161 tactctaaat aattcgagtt gcaggaaggc gacaagagag tgaatcccca ggagcttaca
    11221 taagtaagtg actggggtga gcgaacgcag acgcagcaca tgcaacttga agtatgacga
    11281 gtataaggcc cgacgataca ggacaagaga catgtctaag atttttgatt tcgtaaaacc
    11341 tggcgtaatc actggtgatg acgtacagaa agttttccag gtagcaaaag aaaacaactt
    11401 cgcactgcca gcagtaaact gcgtcggtac tgactccatc aacgccgtac tggaaaccgc
    11461 tgctaaagtt aaagcgccgg ttatcgttca gttctccaac ggtggtgctt cctttatcgc
    11521 tggtaaaggc gtgaaatctg acgttccgca gggtgctgct atcctgggcg cgatctctgg
    11581 tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt ccggttatcc tgcacaccga
    11641 ccactgcgcg aagaaactgc tgccgtggat cgacggtctg ctggacgcgg gtgaaaaaca
    11701 cttcgctgct accggcaagc cgctgttctc ttctcacatg atcgacctgt ctgaagaatc
    11761 tctgcaagag aacatcgaaa tctgctctaa atacctggag cgtatgtcca aaatcggcat
    11821 gactctggaa atcgaactgg gttgcaccgg tggtgaagaa gacggcgtgg acaacagcca
    11881 catggacgct tctgcactgt acacccagcc ggaagacgtt gattacgcat acaccgagct
    11941 gagcaaaatc agcccgcgtt tcaccatcgc agcgtccttc ggtaacgtac acggtgttta
    12001 caagccgggt aacgtggttc tgactccgac catcctgcgt gattctcagg aatatgtttc
    12061 taagaaacac aacctgccgc acaacagcct gaacttcgta ttccacggtg gttccggttc
    12121 tactgctcag gaaatcaaag actccgtaag ctacggcgta gtgaaaatga acatcgatac
    12181 cgatacccaa tgggcaacct gggaaggcgt tctgaactac tacaaagcga acgaagctta
    12241 tctgcagggt cagctgggta acccgaaagg cgaagatcag ccgaacaaga aatactacga
    12301 tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc gctcgtctgg aaaaagcatt
    12361 ccaggaactg aacgcgatcg acgttctgta agatattcct ttctgcttat ctcaaggccc
    12421 gctctgcggg tctttttttc gccaaaagca aaattgcctg atgcgctacg cttatcaggc
    12481 ctacacgtat cctgcaatat attgaatttg caggtttttg taggccggat aaggcgttca
    12541 cgccgcatcc ggcatgacaa agcgcacatt gatatctgtc tgagccccgc tctgcgggga
    12601 ttttttcgcc aaaagcaagg tgattcagat gagaaatctg tgatctattt ggcaaaatta
    12661 tgctttattg tttacccttg tcagactgcc cgtcataagg cggcggagtg tatttctcca
    12721 ttttgagtca gttgaaaagg aatattgaat ggaagatttg aatgttgtcg atagcataaa
    12781 cggcgcggga agctggctgg tagctaacca ggcgctgctg ctaagttatg cagtaaacat
    12841 cgtggcggca ctcgcgatca tcatcgttgg tttgattatc gcgcggatga tttccaacgc
    12901 ggtgaatcgc ctgatgatct cccgtaaaat cgatgccact gttgctgatt ttctctctgc
    12961 attagtccgt tacggtatta tcgcctttac gctaatcgct gcactgggac gcgtgggtgt
    13021 acaaaccgcg tccgtcattg ctgtactcgg tgccgcaggc ttagctgttg gtctggcttt
    13081 gcaggggtca ctgtctaacc tggccgctgg cgtgttactt gtcatgttcc gcccgttccg
    13141 tgccggagaa tatgttgacc tgggcggcgt agccggtact gtgctgagtg tgcagatttt
    13201 ctccaccacc atgcgtactg cagacggtaa aattatcgtt attccgaacg gtaaaattat
    13261 tgccggaaat attattaact tctcccgcga gccagttcgc cgtaacgaat ttattattgg
    13321 cgtggcgtat gattccgata tcgatcaggt taagcagatc ctgaccaata ttatccagtc
    13381 tgaagatcgc attttgaaag atcgcgaaat gactgtgcgc ctgaacgaac ttggtgcatc
    13441 gtcgattaat ttcgtggtcc gcgtctggag caacagcggc gatctgcaaa acgtgtactg
    13501 ggatgtgctg gagcgtatta aacgtgaatt tgatgccgcc ggtatcagct tcccgtaccc
    13561 gcaaatggat gtgaacttta agcgggtgaa agaagacaaa gctgcgtaat caacgctgag
    13621 gccagataat actctctggc ctctctctta ttagttattc tgattgccaa ttaatattat
    13681 caatttccgc taataacaat cccgcgatat agtctctgca tcagatactt aattcggaat
    13741 atccaacgtg ttttcttatt actttcaagg tcttgctctt ggggcggcta tgatcctacc
    13801 gctcggtcca caaaatgctt ttgtgatgaa tcagggcatt cgtcgtcagt accacattat
    13861 gattgcctta ctttgtgcta tcagcgattt ggtcctgatt tgcgcgggga tttttggtgg
    13921 cagcgcgtta ttgatgcagt cgccgtggtt gctggcgctg gtcacctggg gcggcgtagc
    13981 cttcttgctc tggtatggtt ttggcgcttt taaaacagca atgagcagta atattgagtt
    14041 agccagtgcc gaagtcctta agcaaggcag atggaaaatt atcgccacca tgctggcagt
    14101 gacctggctg aatccgcatg tttacctgga tacttttgtt gtactgggca gcctcggcgg
    14161 gcaacttgac gtcgaaccaa aacgttggtt tgcgctcggg acaattagtg cctctttctt
    14221 gtggttcttt ggtctggcta ttctcgcagc ctggctggca ccgcgtctgc gcacagcaaa
    14281 agcacagcgc attatcaatc tggtagtggg atgtgttatg tggtttattg ccttgcagct
    14341 ggcgagagac ggtattgctc atgcacaagc cttgttcagt taggcgctat ctgatggaaa
    14401 aataaaacag aggcgctaag cttgcctcca gaggtcctga attttgggca agtcaattaa
    14461 cacggagaga ctaacgtgaa gttcaaagtt atcgccctgg cggcattaat gggtattagc
    14521 gggatggcag cgcaggctaa cgaattgccg gatagaccgc atattgtcac ctccggtacg
    14581 gcaagcgtgg atgcggtgcc agacattgcc actcttgcga ttgaagtcaa cgtggccgcg
    14641 aaggatgccg ctactgccaa gaaacaggcg gatgagcgcg tcgcacaata catttccttc
    14701 cttgaactca atcagatcgc gaaaaaagat atcagctcag cgaatttacg cacccagcca
    14761 gattacgact atcaggatgg taaaagtatc ctgaaaggct accgcgctgt gagaacggtg
    14821 gaagtcacgc tccgtcagtt agacaaactg aattccttgc tggatggcgc gctgaaggcg
    14881 ggtcttaacg aaattcgttc tgtgtcgctg ggcgtggcgc agccggatgc ctataaagac
    14941 aaagcgcgta aggcagcgat tgataacgcg attcatcagg cgcaggaact ggcgaacggc
    15001 tttcatcgta aactggggcc ggtatatagc gtgcgctacc atgtttccaa ctatcagccc
    15061 agcccaatgg tgcggatgat gaaagccgat gccgcgccgg tgtctgccca ggaaacttac
    15121 gagcaggccg ctattcagtt tgatgatcag gtcgatgtgg tcttccagtt agaacctgtg
    15181 gatcaacaac ccgctaaaac acctgcagca caataatact tgtagcccga cgtattcgtc
    15241 gggctaatgc ctttacccga tattgcctcc tttttctgta attttctgta aaaccatgac
    15301 gtaatcacct atctttgctc gcggtttcat caaggatagg ctgactgaaa cggttaagcg
    15361 gacaaggatg cggggagggg ggatggacat ttttatttcg aaaaagatgc gcaatttcat
    15421 tttattagcg caaaccaata atattgcccg ggcggcagaa aaaatccata tgacggcttc
    15481 gccatttggt aaaagcattg ccgcgctgga agaacaaatt ggctatacgc tatttacccg
    15541 caaagataat aatatcagcc tcaataaagc cggtcaggaa ctgtatcaaa aactgttccc
    15601 ggtttatcaa cgactttctg cgattgataa tgaaatccat aactcagggc gtcgttcacg
    15661 agaaattgtg attggcatag ataatactta tccgacaatt attttcgatc agttgattag
    15721 tctcggagat aagtatgaag gcgtgaccgc ccagccagtt gagttcagcg aaaacggcgt
    15781 tatcgataat ctttttgacc gtcagttaga ttttattatc tccccacagc atgtatctgc
    15841 acgagttcag gaactggaaa acctgactat cagtgaatta ccaccgctgc gattgggctt
    15901 tctcgtttct cgccgttatg aagagcgtca agagcaagag ctgcttcagg agctgccctg
    15961 gctacaaatg cgctttcaaa accgggctaa cttcgaagca atgattgatg cgaatatgcg
    16021 accttgtggg atcaatccca ccattattta tcgtccctac agttttatgg ccaaaatcag
    16081 cgccgtggag cgcggacatt ttctgacggt gatcccgcat tttgcctggc gactggtgaa
    16141 tccggcaacg ctgaaatatt ttgatgcgcc gcacaggccg atgtatatgc aggaatatct
    16201 ttattcaatc agaaatcatc gttataccgc cacgatgctt cagcatattg ctgaagatcg
    16261 tgacgggacg agtcattaac ccagcatcga gccggttgca attaaattac ggtgtaagtc
    16321 gaagacgtgg ctaagatcgt ggtgaatatg tccgccaggc gcattttcca gatagcgatg
    16381 cagataatcc cgatacatag gatgtgcaca attatcaatg atagtgcggg cgcgttgaag
    16441 cggggaaaga ccgcgcagat cggcgatccc ttgttcggtg atgatcactt tgacgctgtg
    16501 ttcgctgtga tcaacatggc tgcacattgg cacgacggtt gagatcttgc cttctttagc
    16561 aatcgacggg gccataaaga tcgacagata cgcgttgcgt tcaaaatcac cgctgccgcc
    16621 gatgccgttc atcagatcga ccccggctac gtgcgttgag ttggcatgcc cgtaaatatc
    16681 aaactccagg ccgacgttca gagcgatgac gcccagacga cggatgattt ccgggttatt
    16741 ggaaatctcc tgcggacgca acacaatgcg gctggcaaag taatccatat tgtcgtaaat
    16801 cttgcgcagg gaatcggccg agattgtcag gctggaggcg ctggccccgc tgattttgcc
    16861 ggtttccagt aaatgcacca ccgattcctg tagcacttcc gaatacatca taaacggagg
    16921 aatttccggg ttttccccca gacgcgccat taccgcatta ttgatattgc ccacgccact
    16981 ttgcagcggc agaaattccg gcggaatacg cccatgcgcc atttcctgca ataagaacgt
    17041 gaccacgtta tcggcaatct gctggcacat gggattttgc ttatccagca tattaccggc
    17101 gtcgggcaag ttggtttcca cgacggcgac aatctttttc ggatcgattt gcacatagcg
    17161 ggtaccgacg cgatccattg catggaagat cgacacgcta ttgcgccgcg gtggcgcgcc
    17221 aggaatcaca atatccgcca gttctgcaac gcgcggatcg tgatagtgat taagttcaat
    17281 gatcactttc ttcgcccgca gcagccatgt cggcgcatta ccgatcccgc tggttaacca
    17341 gactcgacca tccggtgcca gtgccgatgc ttcgatgacg gcaacatcaa tgtcgccgaa
    17401 gaaaccgtaa ttgaccattt gcgccacttc gctcaaatgc aggtcaacga aactcaccgc
    17461 gccctgattg atctttttac gtaaaccgga cgatgtttga tatggcgcac gccaggaaac
    17521 agcatcggcg tcagaaagta catcgtcagc ggcggcgctg attgacgccc ccgtcagcag
    17581 gcgaatttga tacggctttt tggcctcatg ctgttcgtta gctctgcggg caatcgcggt
    17641 gggtagggct ttcggcgaac ccgccggggt aaagccgcta aatgccacca tgtcgttatg
    17701 ctggataatt tctgccgctt catcggcggt catccttgtc cactgagttt ccatttttac
    17761 tccttcgttc gcagggatta atgaccaacg aaattaggtt tacgtttttc gaggaaagcg
    17821 ttcattcctt cctggtaatc ttcgctgtca taaaccgcgc gtcgcatccc ctgaatacgt
    17881 tcaaattcat cggagttcat ggtgtgcgct tcgcccagta cacgcagctc ttctttgata
    17941 acagcaatgg ctaacggcgc tttctcagag atgtggtgcg ccatttgtaa ggtgaaatct
    18001 tccagttctt ccacttccac aacatggttg aggatgccga cagccaacgc gcgctgggcg
    18061 gtgattggcg aagcggtaaa aatcagctct ttgacaatgt ggaagcccgc gtcgcgggtc
    18121 aggttgtgaa tgccgaccag gttatacggg acgccgaggt ttacaggcgt cattgagaag
    18181 gttgaggtac tggcggcgat aatcagatcg gaactcatga tcatttcaaa tgcgccaccc
    18241 caaacactac cttccaccat cgaaatgatc ggtttcggga atttttggat catgcgggtg
    18301 atttgacgca atggatcatc ataggagagc ggatcgcgac cgccagacgg cagttcgtga
    18361 atatcgtggc ctgcggagaa gactttggat ccactcggcg cgcgcaaaat gatacagcga
    18421 atttccggcc ggttgagatc gcttaatgcc tgcataagat catcaataaa gactttactt
    18481 aaggcattaa gtttgcggcc atagttaaac tcaatgaccg ccactttgtt gatagtgaca
    18541 acgttaacat actgataaga cataaaaatt cctttaatca aaatattgcg tctggataaa
    18601 ttcactgagc tgccgcaggc cggtgcgcgg tgagagcgta ttgtttttga ccgctaaaag
    18661 cgtctggcgg taatagcgat cgaaatcttc attcgcgaac aggtgatgca gtacttcttc
    18721 ttcggtctgc ttacgcagcc attccaccga ttgttgttgc cgcacttgtt gtaaacgacc
    18781 actggcagtt agcgcggttt tgaagtcgat gatggcgtgc cagatctcat cgattccacg
    18841 tttttccagt gcgctacaag tcagaacccg tggctgccat tcgtcgtatt tacgtcgcag
    18901 aatatgcagg gcactctcgt acatatgccg ggcaatggcg acattggtat ggttatcgcc
    18961 atcgtctttg ttgataacga gcagatcagc cacttccatc agcccttttt taatgccctg
    19021 cagatcatcg ccgccaccgg caatttgcaa cgagataaaa cagtccacca tgcgggcgac
    19081 ttccgtttcc gactgcccga cgccaaccgt ttcgacaatc actacgtcat aacccgccgc
    19141 ttcgcataac agcattaatt cccgcgctcg ctgactggca ccgcccagat gaccggagga
    19201 tggtaccggg cgaataaacg ccgcttcggc acgcgccagg tcattcatgc gggttttatc
    19261 cccgagaatg ctaccgccag tgaccgggct gctgggatcg accgcaataa ccgcgacctt
    19321 taatccctca cgaatcaaca acatgccaaa ggcttcaaga aaggtacttt tccccgcgcc
    19381 gggtgtgccg gtaacgccca gtcgcagggc gttaccgcag tacggcataa tggcatcaag
    19441 cagttgcgta cttagcgcct gatgacgcgg gtgacggctt tccaccagcg tcatggcctg
    19501 ggcgagtgtg gcacgctcac cctgacgtaa gcggcgaata ctttctgcca gcgtggcttc
    19561 attaatcatg atgctggctt atcagattca gtacgtcgcg cacactctcg agcataggtg
    19621 tacctggacc ataaatcgcc gccacgccgc gctcttgcag gaaggcgtaa tcctgcggtg
    19681 gaatgacgcc accggcgact acgcagatat cttcgcgtcc ccattttttc agcgcttcga
    19741 ccagttccgg gatcagcgtt ttatgaccgg cagccagtga ggatgcgccc actacgtgaa
    19801 cgtcgttttc tacggccagg cgggcgatct cttcaggtgt agagaacatc gggcttaaat
    19861 ctacgtcgaa accgagatcg gaataggcgc tggcgatcac tttcgcgccg cgatcgtgtc
    19921 catcctggcc catcttagcg atcagaatgc gcgggcgacg accattgtcg gcaaggaact
    19981 gctccgtttg cgcaacaatg gcatcgaact cggaggccga tttctcagac tgatgatagc
    20041 tttgcgcaat cacgccggta acacactggc ttggcaccag ataacggtcg aaagcgactt
    20101 ccagcgcatc ggaaatttca cctagggtgg cgcgaacgcg agcggcatta acagcggcag
    20161 ccagcaggtt ttcgttatgc tgtgcggcgt gagtcagggc gttcaacgcg gcggttacgg
    20221 cggcatcatc acggggggcg cgaatgcgtt ccagcgaagc aatttgctcg ttacgcacca
    20281 tcacgttgtc gatctcaagt acatcggttt cgtcttcgtg atccagtttg tacttgttga
    20341 caccaacgat gacacgcttg ccctggtcga tcagcgactg ttcgcgcgct gaggcctctt
    20401 cgatcattcg ttttggcaga cctgcttcga tcgctttcgc catgccaccg gcttcgtcga
    20461 tctattggat aatagctctg gcttgtttga cgatttgatc ggtcagcgac tcaatgtaat
    20521 aggatccggc cagtggatcg acggtgcggc agagttctga ttcttcctgg atgatgatct
    20581 gggtgttgcg ggcaatgcgt gctgagaaat cggtaggcaa accaagcgct tcgtcaaagg
    20641 cgttggtatg cagtgactga gtaccgccca gcgtcgcagt cagcgcttca atggtggtgc
    20701 ggataacgtt gttatacgga tcctgttcag tcaggctcca gcctgaggtc tggcagtggg
    20761 tacgcagcgc cagtgatttc gggtcctgtg cgccaaatcc actgacctct tcgctccata
    20821 aataacgtgc cgcacgcaac atggcgacgt tcataaacag atccatgccg atgccgaaga
    20881 agaacgacag gcgaggagcg aagtcatcaa ttttcagtcc ggcagagatt gctgctttga
    20941 tgtactcaat cccatcagcg agcgtaaatg ctacctgctg cacgcagttg gcacccgctt
    21001 cacccatgtg gtaaccgctg atactgatgg tattaaatcg cggcatgttg ccggaacacc
    21061 aggcgatgat gtcggcgata atgcgcattg acggttttgg cgggtaaata taggtgttgc
    21121 ggcagaggta ctctttgaga atatcgtttt gaatggtgcc ggtcagttta tcaggtgtaa
    21181 caccttgctc ttctgcggcg acgatataaa acgccagtac tggtagcact gcgccattca
    21241 tggtcatcga aaccgacatt ttatccagcg ggatctggtc gaacaggact ttcatatctt
    21301 ccacggtgtc gatagcgacg cccgctttgc cgacgtcacc cgccacgcgt gggttatcgg
    21361 agtcgtagcc acggtgggtg gcaaggtcaa acgcaacgga aagacctttt tgcccggcgg
    21421 ccaggttacg gcgataaaaa gcgttggact cttttgctgt tgaaaaacca gcatactgac
    21481 ggatggtcca cggttgggcg gtatacatag tggcacgcgg gccacgaacg tagggcggca
    21541 aaccaggaag ggtacctgtc acctccagat tatcgagatc ggcttcggta tacagcggct
    21601 tgatggcgat cccttccgcg gtttgctgaa ccagcgagtc gacagttttc tcccgacggc
    21661 tcaattcctt gttggcaagc tgttgccact cctgctcgtt agacattatt tgctccgtaa
    21721 aacgcaatca aaatgagggt atttgcgctt acggtgagct ttttacgtgg gtttgtcgcc
    21781 agcaaaaaaa atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat
    21841 gtgattcaag gaatacaaaa agaagtgagg acaacgcctg atatgtgcta tcaggcatta
    21901 tttgatggat taatcctgac gaaggacttt gtgaccataa tcgagtaacg catcagtgac
    21961 tttacgcatc atgcggcttt caggagcaaa gcggtgccag tagagcatcc gtcgttgaaa
    22021 tagcccaggc gttaagtcaa tcagttcacc gctggccagc tctttctcga tttgcaggtg
    22081 cgggatcata cagcaggtgg tgccctggcg agcaagttgt acgaacgctt ctgaagaatt
    22141 aacgatatgg cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg
    22201 gtgcatatcg tcaagatggt caaacgcgac cactggcgct ttcagtaatg ccgaacgcgt
    22261 tacgccgtta gggaaatatt tttcggcaaa gggttttgag ctgacgaaca gatagtcgag
    22321 cgcaccaagt ttatcgacaa gacaactcgg cagcgcctga tgttgaatac tcaccgcgcc
    22381 gaccacttcg ccgcggcgca gacgttcctg agtgcgggtt tcatcttcta cctgcaagtt
    22441 gaggcggata ggcgaatcag ccaacacagg agccagtgca ggaagcaacc acgtcgccag
    22501 actgtcggcg ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc
    22561 cagccactct tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg
    22621 ttccgtcggg cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag
    22681 ttgcttaatg cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc
    22741 aaatcctcgt tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt
    22801 cattgctgtt gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc
    22861 aggttgcata taccgaagcc gtcggcgggt aacatttgta ttcgtggtta aacgcgcagc
    22921 ttaaaagatc agggatttgc gctaatgcat ttcgctcagc gagttcgcgc cctggttgtg
    22981 ttgaatggcg tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg
    23041 tatgctttat ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag
    23101 ttaaccacgc ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa
    23161 atttcatacc acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg
    23221 ggcggcactt cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc
    23281 cgcacacttt attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag
    23341 ttcagatgct tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga
    23401 agtcgacagc cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat
    23461 gatcaaaggc ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa
    23521 atttatctgt attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgcc
    23581 agtagaagtt atcccgatgg cacgtagtgc agtggcgcgc cagctggtga aactgggcgg
    23641 tcgtccggaa taccgtcagg gcgtggtgac cgataatggc aacgtgatcc tcgacgtcca
    23701 cggcatggaa atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt
    23761 ggtgactgtt ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga
    23821 cggtgtcaaa accattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct
    23881 cttcattaaa tttggtgaca tgtgtcacgt ttttacctgg ctattgtcga ttgctctaaa
    23941 taaatcttct aaaccagcat attcatccaa gaattacctt tgcgtgatat ttcctcaaca
    24001 tcgcgacgca aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggacg
    24061 caaatccgca cacaacattt caaaagacag gattgggtaa atggcaaagg tatcgctgga
    24121 gaaagacaag attaagtttc tgctggtaga aggcgtgcac caaaaggcgc tggaaagcct
    24181 tcgtgcagct ggttacacca acatcgaatt tcacaaaggc gcgctggatg atgaacaatt
    24241 aaaagaatcc atccgcgatg cccacttcat cggcctgcga tcccgtaccc atctgactga
    24301 agacgtgatc aacgccgcag aaaaactggt cgctattggc tgtttctgta tcggaacaaa
    24361 ccaggttgat ctggatgcgg cggcaaagcg cgggatcccg gtatttaacg caccgttctc
    24421 aaatacgcgc tctgttgcgg agctggtgat tggcgaactg ctgctgctat tgcgcggcgt
    24481 gccggaagcc aatgctaaag cgcaccgtgg cgtgtggaac aaactggcgg cgggttcttt
    24541 tgaagcgcgc ggcaaaaagc tgggtatcat cggctacggt catattggta cgcaattggg
    24601 cattctggct gaatcgctgg gaatgtatgt ttacttttat gatattgaaa ataaactgcc
    24661 gctgggcaac gccactcagg tacagcatct ttctgacctg ctgaatatga gcgatgtggt
    24721 gagtctgcat gtaccagaga atccgtccac caaaaatatg atgggcgcga aagaaatttc
    24781 actaatgaag cccggctcgc tgctgattaa tgcttcgcgc ggtactgtgg tggatattcc
    24841 ggcgctgtgt gatgcgctgg cgagcaaaca tctggcgggg gcggcaatcg acgtattccc
    24901 gacggaaccg gcgaccaata gcgatccatt tacctctccg ctgtgtgaat tcgacaacgt
    24961 ccttctgacg ccacacattg gcggttcgac tcaggaagcg caggagaata tcggcctgga
    25021 agttgcgggt aaattgatca agtattctga caatggctca acgctctctg cggtgaactt
    25081 cccggaagtc tcgctgccac tgcatggtgg tcgtcgtctg atgcacatcc acgaaaaccg
    25141 tccgggcgtg ctaactgcgc tgaacaaaat cttcgccgag cagggcgtca acatcgccgc
    25201 gcaatattta caaacttccg cccagatggg ttatgtagtt attgatattg aagctgacga
    25261 agacgttgcc gaaaaagcgc tgcaggcaat gaaagctatt ccgggtacca ttcgcgcccg
    25321 tctgctgtac taattcccct tctctgaaaa ttaacgggca ggtcactgac ttgcccgttt
    25381 ttttatccct tctccacacc gttgccgcgt tgcgcattct actttgcgag tctgctccag
    25441 aaaataaatc aaagtcagat caatgcgttg tgtttattta atggggattt acgatggcgg
    25501 gacagcatgg ggctgtaacg gtaaagccct caccgaagcg agggcttaaa ggagaagggt
    25561 tatgatgcga cttgtcatca tactgattgt actgttactg ataagtttcg gagcttatta
    25621 acagtcagtc tcaggggagg agtcatcctc ccttaccctt actcactaaa ttaggtcaaa
    25681 gaatcaacga tgtcaatcag ggcgatgcgg ggtgcatcgc cctgaaccga actttttgat
    25741 agccacttca tcgcccacgt ttactgccgt aatagatcct ttttcccaaa aattatctct
    25801 gttttgcatg gggtttttcg agcacgctcg aagaaaagat ttaaaggtag atcaatgcgt
    25861 tgtggttatt taaagagagc ttacgatagc tgggcagcat ggggctgtaa cggtaaagcc
    25921 ctcaccgaag cgagggcttg aaggagaagg gttatgatgc gacttgccat catactgatt
    25981 gtactgttac tcataagttt cccagcttat taacagtcag tcacagggga ggagcaatcc
    26041 tcccttaccc ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc
    26101 aggtgtatcg cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga
    26161 tatcccactc ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg
    26221 gttgcgtttt atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc
    26281 ccaggcgctg accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg
    26341 aaaggggtag cacgtcacgc acatccagtt ttggctcatg gatcttcaac ctgttcatca
    26401 ccagttcgct ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca
    26461 aaactggaag atatacgcgc ttaccggcgc gccagagttg ttctattagc ggctgggtgt
    26521 cgagttcacc atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat
    26581 aagtcatcat ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg
    26641 cacgacgacg ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt
    26701 ctggtagttg cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag
    26761 gctgtaaccc ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc
    26821 ggacggaccg agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg
    26881 gctcagggga ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct
    26941 accacagtaa accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc
    27001 ttgttcaagt aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact
    27061 tgccgcgtag tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc
    27121 ggcaatgaag accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa
    27181 ccgttggttc agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt
    27241 cacacgcagt gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc
    27301 ctgctgattg actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta
    27361 tagctaccct gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag
    27421 catatcatga atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc
    27481 ctggttacaa cgaaatgaac cagtatctga accaacaagg gacgggtctg acccctgctg
    27541 agatgcatgg tttaatcagt gggatgatat gtggcggtaa cgatgacagc tcatggctac
    27601 cgctacttca cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcac
    27661 tgcgtaaaat gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc
    27721 tttatctgcc tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt
    27781 gggtcaatca cttcctgctt ggtcttggcg ttacgcaacc gaagctggat aaagtgaccg
    27841 gcgaaaccgg tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag
    27901 acgaagatca ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg
    27961 ccgcgctgtt atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa
    28021 aaccgactct acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg
    28081 caagagtttc agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg
    28141 ctgatttttg ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag
    28201 aacagtgact tctggtactt caccggcttt aacgaaccgg aagcggtgct ggtgctgatt
    28261 aaaagcgatg acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg
    28321 gagatctggt ttggccgtcg tttaggtcag gatgccgcgc cagagaaact gggcgttgac
    28381 cgcgcactgg cattcagcga aattaatcag caactttatc aattacttaa tggtctggat
    28441 gtggtttacc acgcccaggg cgaatatacc tatgctgatg aaatcgtgaa cagcgcgctg
    28501 gaaaagctgc gtaaaggttc gcgacaaaat ctcaccgcgc cggcaacgat gatcgactgg
    28561 cgtcccattg ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtacttcgc
    28621 cgcgcgggtg aaatcaccgc cctggcgcat acccgggcga tggaaaaatg ccgtccggga
    28681 atgttcgagt accacctgga aggcgaaatt caccacgaat ttaaccgcca cggcgcgcgc
    28741 tatccttcct ataacaccat tgtcggcagc ggtgaaaacg gctgcatttt gcactacacc
    28801 gaaaacgagt gtgaactgcg tgacggtgac ctggtgttga ttgacgcggg ttgtgaatac
    28861 aaaggttacg ctggcgatat tacccgcacc ttcccggtca acggcaaatt cactcaggcc
    28921 cagcgtgaaa tctacgacat tgtgctggag tctctcgaaa ccagcttgcg cctgtatcgt
    28981 ccgggaactt ccatgcagga agtcaccggt gaagtggtgc gcatcatggt tagcggcctg
    29041 gtaaaactcg gcattctgaa aggtgatgtt gatgaactga tcgctcagaa cgcccatcgt
    29101 cctttcttta tgcatggcct tagccactgg ttagggctag atgtccatga cgttggcgtt
    29161 tatggtcagg atcgctcgcg cattctggaa ccgggcatgg tactgaccgt agagccaggg
    29221 ctgtatattg cgccggatgc agaagtgcca gaacaatatc acggtatcgg cattcgtatt
    29281 gaagacgaca ttgtgattac cgaaaccggt aacgaaaacc tcaccgccag cgtggtgaaa
    29341 aagccggaag aaatcgaagc gttgatggca gctgcgagaa agcaatgagc gtaatcatcg
    29401 tcggtggcgg catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacgggg
    29461 cgctgccggt acatttgatt gaagcgaccg cgccagagtc acatgctcat ccgggctttg
    29521 atggacgagc tattgcgcta gcggcgggga cctgtcagca actggcgcgc atcggcgtct
    29581 ggcaatctct ggctgattgc gcaaccgcca tcactaccgt gcatgtcagc gatcgtggtc
    29641 atgctggatt cgtcaccctc gccgcagaag attaccaact ggcggcgctg gggcaggttg
    29701 tcgaattgca taatgtcggg caacggctgt ttgcattgct gcgaaaagca cctggcgtaa
    29761 cgctgcattg ccctgatcgc gtagctaacg ttgcccgtac tcagagtcac gttgaagtga
    29821 cgctggagag cggcgagatg ctgacgggcc gcgtgctgat agccgctgat ggcacccatt
    29881 cagcgttagc caccgcgtgc ggcgttgatt ggcagcagga gccttacgaa caactggccg
    29941 tgattgccaa cgttgcgact tccgttgcgc acgaaggccg cgcttttgaa cgcttcacgc
    30001 aacatggccc gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc
    30061 atccactgga acggcgcgaa gaggtgctgt cgtggagcga cgagaagttt tgccgtgaac
    30121 tccagtcggc ctttggctgg cgacttggaa aaattaccca cgccggtaaa cgcagtgctt
    30181 atccgctggc gttaacccgc gccgccagag ctattaccca tcgtaccgtg ctggtgggca
    30241 atgcggcgca aactctgcac ccgattgccg ggcaagggtt taacctcggt atgcgagatg
    30301 tcatgagcct tgcggaaacc ctaactcagg cgcatgagcg cggagaagac atcggtgatt
    30361 acggcatatt gtgccgctat caacagcgtc gacagagcga tcgcgaagca accatcggcg
    30421 tcacggacag cctggtacat ctttttgcca accgttgggc accgctggtt gtcgggcgca
    30481 acatcggact gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca
    30541 ccctcggttg ggtggcgcgt tgaaggcggt tttaaggaat aaacaatgca aagtgttgat
    30601 gtagccattg ttggtggcgg catggtgggg ctggcggttg cctgcggctt acaggggagc
    30661 ggcttacgcg ttgccgtact ggagcagcgc gtaccggaac ctctggcggc ggatgcacca
    30721 ccacaactgc gcgtttcggc tatcaatgcc gccagcgaaa aattactcac ccgtcttggc
    30781 gtctggcagg acattctctc tcgtagggcc agttgttatc acggtatgga agtgtgggac
    30841 aaagacagtt ttggtcacat ttcgtttgac gatcaaagca tgggctatag ccatcttggg
    30901 catatcgttg aaaactcagt gattcactac gcgttgtgga acaaagcgca gcagtcgtca
    30961 gatatcactc tgttggcccc cgcagaatta cagcaggtcg cctggggaga aaatgaaact
    31021 ttcctgacgc tgaaagatgg cagcatgtta acggcgcgtc tggtgattgg cgcggacggt
    31081 gctaattcct ggttgcgcaa caaagcagat attccgctga ctttctggga ttatcagcat
    31141 cacgcgctgg tagcgaccat tcgcacggaa gaaccgcatg atgcggtggc gcggcaggtt
    31201 ttccatggcg aaggcattct ggccttttta ccgcttagcg atccgcatct ttgctcaatt
    31261 gtctggtcac tgtcgccaga ggaagcgcag cggatgcagc aggcaagtga agatgaattt
    31321 aatcgcgcgt taaatatcgc ttttgataat cgcctgggct tatgcaaggt tgagagcgcg
    31381 cgtcaggtgt tcccactgac ggggcgttat gcgcgccagt ttgccgcgca ctgtctggtg
    31441 ctggtgggcg acgccgcgca taccattcac ccgctggcgg ggcagggagt aaatctcggc
    31501 tttatggatg ctgcagagct gattgctgaa ctgaaacggt tgcatcgtca ggggaaagac
    31561 atcgggcagt acatttacct gcgccggtat gagcgtagcc gcaagcacag tgcggcgctg
    31621 atgctggctg gtatgcaggg attccgcgat ctgttttccg gtaccaatcc ggcgaaaaaa
    31681 ctgctgcgtg atattggttt gaaactggcc gacacgcttc ctggcgttaa accacaactt
    31741 atccgccagg caatgggatt aaacgatttg cctgaatggc tgcgttaaaa atttctcttc
    31801 tgctgtttat ttgataccca tcacactttc atctcccggt tttttcgccg ggagattttc
    31861 ctcatttgaa ataaactaat ttcacctccg ttttcgcatt atattttcta atgtcattat
    31921 tttttgattt agtgtttttt gacatttttt tagctcttaa tattgcctta ttcaaattag
    31981 ctttctaatg gcatcatctt tatatagaaa ctggtgtgtt ttttggtttt ttattctgtc
    32041 gcgattttgg cgttttttaa ccataagcta atgcgatgat caattttacc ttatggttaa
    32101 cagtctgttt cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg accgggagtg
    32161 gctccgatgc tgggtttcgt ggtgataatt tcaccatgaa aagttgtcag ccccgcttat
    32221 tcaatgagga caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg
    32281 ctcgcatggt ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg
    32341 aacatcatgc ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg accatcgtcg
    32401 atctccgcgg cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga
    32461 agctcaccaa aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga
    32521 tagatgacct catcgtctac tactttactg aagatttctt ccgcctcgtt gttaactccg
    32581 ccacccgcga aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa
    32641 ttaccgttcg tgatgacctt tccatgattg ccgtgcaagg gccgaatgcg caggcaaaag
    32701 ctgccacact gtttaatgac gcccagcgtc aggcggtgga agggatgaaa ccgttctttg
    32761 gcgtgcaggc gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg
    32821 aaattgcgct gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagcgggtg
    32881 ttaagccatg tggcttgggc gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt
    32941 atggtcagga gatggacgaa actatttctc ctttagccgc caacatgggc tggactatcg
    33001 cctgggaacc ggcagatcgt gactttatcg gtcgtgaagc cctggaagtg cagcgtgagc
    33061 atggtacaga aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac
    33121 tgccggtacg ttttaccgat gcgcagggca accagcatga aggcattatc accagcggga
    33181 ctttctcccc gacgctgggt tacagcattg cgctggcacg cgtgccggaa ggtattggcg
    33241 aaacggcgat tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt
    33301 ttgtgcgtaa cggtaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa
    33361 cgtaccagca gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg
    33421 cacttacacc gttggtataa ccgaacatgc ccaggagctg ttaggcgata tggtgtttgt
    33481 tgacctgccg gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc
    33541 agtaaaagcg gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaatga
    33601 cgcactgagc gattccccgg aactggtgaa cagcgaaccg tatgcaggtg gctggatctt
    33661 taaaatcaaa gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga
    33721 agcattgtta gaagacgagt aacggcttta ttcctcttct gcgggagagg atcagggtga
    33781 ggaaaattta tgcctcaccc tcactctctt cgtaaggaga gaggttcaca attcactgca
    33841 cgtttcagga accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt
    33901 ttattgaacg ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg
    33961 gtgcacaatc gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga
    34021 ccccgccgca ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta
    34081 ttgccagtcg caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc
    34141 taccgccggt tatcctgcgt aacatgctgg aaaatccggg ctggtatacc gcgtacactc
    34201 cgtatcaacc tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa
    34261 cgctggattt gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg
    34321 ccgccgaagc aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct
    34381 tcgtggcttc cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct
    34441 ttggttttga agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg
    34501 gcgtgctgtt acagcaggtt ggcacaaccg gtgaaatcca cgactacacc gcgcttatta
    34561 gcgaactgaa atcacgcaaa attgtggtca gtgtcgccgc cgatattatg gcgctggtgc
    34621 tgttaactgc gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg
    34681 gcgtgccgat gggctacggt ggcccacacg cggccttctt tgcggcgaaa gatgaataca
    34741 aacgctcaat gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc
    34801 tgcgcatggc gatgcagact cgcgagcaac atattcgccg tgagaaagcg aactccaaca
    34861 tttgtacttc ccaggtgctg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc
    34921 cgattggcct gaaacgtatc gctaaccgca ttcaccgtct gaccgatatc ctggcggcgg
    34981 gcctgcaaca aaaaggtctg aaactgcgcc atgcgcacta tttcgacacc ttgtgtgtgg
    35041 aagtggccga caaagcgggc gtactgacgc gtgccgaagc ggctgaaatc aacctgcgta
    35101 gcgatattct gaacgcggtt gggatcaccc ttgatgaaac aaccacgcgt gaaaacgtaa
    35161 tgcagctttt caacgtgctg ctgggcgata accacggcct ggacatcgac acgctggaca
    35221 aagacgtggc tcacgacagc cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc
    35281 tcacccatcc ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc tatatgcact
    35341 cgctggagcg taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca
    35401 tgaaactgaa cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc
    35461 acccgttttg tccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg
    35521 actggctggt gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac
    35581 agggcgaata cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc
    35641 atcgcgatat ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata
    35701 tggcaggaat gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg
    35761 atctgcgcgc gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc
    35821 cttctaccca cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt
    35881 tcggcggtca ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc
    35941 cgggctttat tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc
    36001 acggcggtgg tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg
    36061 taccgggtca tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg
    36121 cggcaccgtt cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg
    36181 gcgcagaagg gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca
    36241 gccgcctgca ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg
    36301 aatgtattct cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg
    36361 ccaagcgcct gatcgactac ggtttccacg cgccgaccat gtcgttcccg gtggcgggta
    36421 cgctgatggt tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg
    36481 cgatgctggc tatccgcgca gaaatcgacc aggttaaagc cggtgtctgg ccgctggaag
    36541 ataacccgct ggtgaacgcg ccgcacattc agaacgaact ggtcgccgag tgggcgcatc
    36601 cgtacagccg tgaggttgca gtattcccgg caggtgtggc agacaaatac tggccgacgg
    36661 tgaaacgtct ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta
    36721 gcgaatacca gtaattcact gattcgacta ttttctaaag gcgcttcggc gcctttttag
    36781 tcagatgaca aagtacaaaa gtgctcagac agtcccctcg cccctttggg gagagggtga
    36841 ggggaacagg ccggcactgg cgcgaatatt taccctcacc ccggccctct ccctgaaagg
    36901 gcgaggggga aaagcgtgcc aacattgaag attgagccag tttgttagca atctcaaaga
    36961 tacgtcaacg aatgaatttt tctcggaaaa acaaatggtt atagcacttg tgaccggtgg
    37021 cagtcgcggc atcgggcggg caactgcatt actgttggcg caagaagggt atacggtggc
    37081 ggttaattat cagcaaaacc tccacgcggc gcaggaagtg atgaacttaa taacgcaagc
    37141 cggtggcaaa gcattcgtgc tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat
    37201 gtttacagca atcgatcagc acgatgaacc gctagcagcg ctggtcaata acgccgggat
    37261 cttgtttacc cagtgcaccg ttgaaaacct taccgcagag cgaatcaacc gagtactttc
    37321 caccaacgtg acgggatatt ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa
    37381 aaatggtggc agtggcggcg ctatcgtcaa tttctcttcg gtggcctcac ggttgggttc
    37441 gccaggggaa tatgttgatt acgcggcatc gaaaggggcg attgatacgt taaccaccgg
    37501 actatcgctg gaagtcgccg cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat
    37561 ttataccgaa atgcacgcca gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa
    37621 catccccatg cagcgtggtg gacaggcaga agaggtcgcg caggccattg tctggctact
    37681 aagtgataaa gcctcttacg tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa
    37741 cagggaagtt gtctgaccgg atgcaacaag tattgcatcc ggtacttcat cgacttaaag
    37801 cttctcgccg ttgctggcaa tcacctcttg gtaccagtta aagctcttct tacgtgaacg
    37861 cgacatatcg ccagcaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct
    37921 gtactgcccg gtggtgaacg atacgcagtc gatgcagccc cacggtgtgt agcccatcag
    37981 atccacgcca tcgtaagtca ccgctttttt catctcttcg atatgggcgc gcaggtagtc
    38041 aatgcggtag tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc
    38101 gttttcgaca ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg
    38161 cagacctact ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac
    38221 gctgccttcg aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt
    38281 catgtaatag ctgaaaccaa gataatcgca ggtgccttca cgcagtacat cgagatcgcc
    38341 gtcttccatt ttgatgttaa atccgcgacg ctcccactcg ttcaacacat aggacgggta
    38401 atagccgcgt agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa
    38461 catcacatcg tccgggttac agcagtaggg atagagcggc accatcgcca gcatacagcc
    38521 gactttcatc tccgggttaa tgcgacgcgc agctttcacc gccagggcgc tggcgacaaa
    38581 ctggtgatgc agcacctggt acatcgtctc ttccgggttg tcatgctcgg tataaaccac
    38641 gccggagcag cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt
    38701 gaaggtcatc cagtatttga ctttatgctt atagcgttca aacaccactt ccgcgaaacg
    38761 gacaaagaaa tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag
    38821 cggcatttca aagtgggaga gggtgatcac cggttcgatg ttgtatttca gcagttcatc
    38881 gaacatatca tcgtagaact tcagcccttc ttcgtttggc tgagcttcat caccttttgg
    38941 aaaaatacgc gtccaggcga tggaagtccg aaaacatttg aagcccattt cggcaaatag
    39001 cttgatgtct tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt
    39061 gcctggcaag acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac
    39121 gtcacaaatg ctcggctctt tgccgccttt gttccagctg ccttcgacct gatgagcggc
    39181 aactgcgccg ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc
    39241 cagatgagtc aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc
    39301 aacgtttcca ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg
    39361 aatcgcactt caaaacgggt gatgtgcttc gtgtcgggcg ttttgaagat gacggttatt
    39421 tttgcacgat tgaagtcacc gcaacctcaa ccgtaaggct ggatacgctg actgaaaaac
    39481 atgcagagca ggaaaatatg accctgactg aactgaaaaa ggtcattgcc gacatctatc
    39541 ccggtcagac acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg
    39601 ttagttgaaa tttctcattt aatttcatga tttttaggag attagtctca aatcagaatt
    39661 tattttagct aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg
    39721 tgcgtgtacg tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat
    39781 tcgctggcag aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc
    39841 gttgggctgg tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata
    39901 accagctaca gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat
    39961 cacgccattc ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt
    40021 tacctgttga ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta
    40081 gcgcgcgggt tgatgattgt tatctggagc ctggcattgc tgggtattct gtttaaactg
    40141 accatcgcgc accgattcaa aattttatct ctggtgacct atctggcgat gggctggctg
    40201 tcgctggtgg taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg
    40261 gcggtaggcg gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca
    40321 tacaaccatg ccatctggca cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg
    40381 atctatttgt atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc
    40441 cagcgtcgca tcaggcatcg gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat
    40501 ctacgggcct cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc
    40561 agcgtcgcat caggcgataa atgcctgatg cgatacagag atttactctt cgagcgaata
    40621 cggcagcggc tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg
    40681 ttccatatcg ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc
    40741 agccagcacc gtaccggtac gacgccagtt ctcgcccatt ttcagcccta agtcttcacc
    40801 agcttccggc agtcggctgg cgctacctgc cagcaaccag agcgcgcgtt tgttggcacc
    40861 acggaatttt gctcgcgcca ccatctcttg tccggtataa cagcctttct taaagctgat
    40921 accgcccagc gcctggagat tggtcgcctg tgggataaac tgcccgctgt tggcggcatc
    40981 aatcaccggg aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc
    41041 cgcttcaccg cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag
    41101 gaaacgttct gccgggtgtt caaaccatag caaagtggtc gcgccttctt tgactacctg
    41161 tttttctttc gaaggcagtt cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc
    41221 ggcaacacca agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc
    41281 atattttttc agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa
    41341 gccatcgccg tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg
    41401 ggcggcgagc aggtgctgat cttctgccat ctggctgaca tctgctgtca cctgaccctg
    41461 catatatttt tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat
    41521 cagcgtcagc ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc
    41581 cataatagag tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga
    41641 ggcaatgcaa gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa
    41701 tgaagccatt gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc
    41761 cgtaaaagaa ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga
    41821 agaagatgga cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg
    41881 aactcgatat ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg
    41941 aaaaacgcat ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga
    42001 tgaatcacgg taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac
    42061 ggaaccggga acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg
    42121 ctttccttgc tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc
    42181 agttacaccc cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc
    42241 cagcggcgta ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt
    42301 tggcaagggc aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg
    42361 ctgcgtttac gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg
    42421 gacgaagctg aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa
    42481 agatgacggc aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat
    42541 ttcgttgagg atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt
    42601 agtttcatcc agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct
    42661 ggtaaattca tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag
    42721 tttgtcatgc agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc
    42781 aagcccatac agcgcaacaa ttttaccttc aaggttcagg tcgtcgagct gatcccagac
    42841 ggcttcccag tcttcctgga tttcaccaaa atcccaggtc gggataccca gaatgagcac
    42901 atcgtactgc tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc
    42961 tgggccgata atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc
    43021 gtaaaaaaga cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcagcta
    43081 gtgtatcaga atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca
    43141 taaggggcgc aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg
    43201 ctggaaaaaa atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg
    43261 gtggagtggt tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag
    43321 gcattactgg cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg
    43381 agtgcagtgc gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc
    43441 agtgcgcatc tcgcttcacc gaaattgcca cagcgtttgc caaaagattt aagcgaagcg
    43501 caggtcgaac gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa
    43561 gccatgcttg aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cggactgaca
    43621 atgagtgata tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag
    43681 cgcctggtgc cgttaggtga agaggcggtt tactggctgg aaacgtatct cgagcatggg
    43741 cgaccgtggt tgttgaacgg tgtctctatt gatgtgttgt tccccagcca gcgtgcgcag
    43801 cagatgacgc gacagacctt ctggcaccgc attaaacatt atgccgtgct ggcgggtatc
    43861 gacagcgaaa agctgtcacc gcatgtgttg cgtcacgctt ttgccactca cttattaaat
    43921 catggtgcgg atttgcgcgt ggtgcagatg ctactgggcc acagcgatct ctccaccacg
    43981 caaatttata cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg
    44041 cgggcgtgat gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact
    44101 ttgttagcgg cgttttcagg ctttgctcag gctgatgacg cggcaattca acaaacgtta
    44161 gccaaaatgg gcatcaaaag cagcgatatt cagcccgcgc ctgtagctgg catgaagaca
    44221 gttctgacta acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg
    44281 ccaatgtatg acgttagtgg cacggctccg gtcaatgtca ccaataagat gctgttaaag
    44341 cagttgaatg cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc
    44401 atcaccgtgt ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca
    44461 gactacaacg cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac
    44521 agcgatgcag agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt
    44581 gatgatgtga tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac
    44641 cattacgcac ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat
    44701 ggcacacttg ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac
    44761 caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg
    44821 cagtcgatga aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg
    44881 ccagccgggg cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct
    44941 ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag
    45001 gaacgcggat tattgtggtc ggcgattttg acgccgacgg cgccaccagc acggctctaa
    45061 gcgtgctggc gatgcgctcg cttggttgca gcaatatcga ctatctggta ccaaaccgtt
    45121 tcgaagacgg ttacggctta agcccggaag tagtcgatca ggcccatgcc cgtggcgcgc
    45181 agttaattgt cacggtggat aacggtattt cctcccatgc gggcgttgaa cacgctcgct
    45241 cgttgggcat tccggttatt gttaccgatc accatttgcc gggcgaaaca ttacccgcag
    45301 cggaagcgat cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag
    45361 gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct
    45421 ggtttgatga gcgcggcatc gcaattccta acctggcaga actgctggat ctggtagcgc
    45481 tggggacagt agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg
    45541 ggatgagtcg catccgtgcc ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg
    45601 caaatcgcga tgcacaaaaa ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc
    45661 tcaatgctgc cgggcgactg gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca
    45721 acatcggcga agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag
    45781 agatcgaaca aggaatgcag gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc
    45841 gtgacacgct acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg
    45901 gtattctggc ttcgcgtatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag
    45961 caggtgacgg tacgctgaaa ggttccggtc gctccattca ggggctgcat atgcgtgatg
    46021 cgctggagcg attagacaca ctctaccctg gcatgatgct gaagtttggc ggtcatgcga
    46081 tggcggcggg tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg
    46141 aactggttac tgagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc
    46201 cgttaagtcc agccgaaatg accatggaag tggcgcagct gctgcgcgat gctggcccgt
    46261 gggggcagat gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc
    46321 tggtgggcga acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg
    46381 atggtattgc ttttaatgtc gataccgccc tctggccgga taacggcgtg cgcgaagtgc
    46441 aactggctta caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca
    46501 tcgacaatat ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta
    46561 caatcccgct cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg
    46621 aaattaatcc ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg
    46681 ggtatctttg actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa
    46741 cagccggatg tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc
    46801 ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt
    46861 ctgctggaac tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa
    46921 ctcgacgccc tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa
    46981 tatgacagcg ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag
    47041 gactgggcga gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa
    47101 actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa
    47161 atctccggcg attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg
    47221 cgtaaaagcc cgtttgactc cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt
    47281 tatccggaag ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac
    47341 gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt
    47401 attacccaca tcccgaccgg gatcgtgacc cagtgccaga acgaccgttc ccagcacaag
    47461 aacaaagatc aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag
    47521 aaaaatgccg agaaacaggc gatggaagat aacaaatctg acatcggctg gggcagccag
    47581 attcgttctt atgtccttga tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc
    47641 cgcaacacgc aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa
    47701 gcagggttat gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc
    47761 gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga
    47821 attgccttcc cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc
    47881 gacggtaaag agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc
    47941 atgatgaccc gccgtattat gggtaaagcc tcttttgtta ccctgcagga cgttggcggt
    48001 cgtattcagc tgtacgttgc ccgtgacgat cttccggaag gcgtttacaa cgagcagttc
    48061 aaaaaatggg atctcggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc
    48121 ggcgaactgt ctatccactg caccgagctg cgtctgctga ccaaagcact gcgtccgctg
    48181 ccggacaaat tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat
    48241 ctcatctcta acgatgaatc ccgcaacacc tttaaagtgc gttcgcagat cctctctggc
    48301 attcgccagt tcatggtaaa tcgcggcttt atggaagttg aaacgccgat gatgcaggtg
    48361 atcccaggcg gtgcggctgc gcgtccgttt atcacccatc ataacgcgct ggatcttgac
    48421 atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag
    48481 cgtgtattcg aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca
    48541 gagttcacca tgatggaact ctatatggct tacgcagatt acaaagatct gatcgagctg
    48601 accgaatcgc tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac
    48661 ggcgacgtga cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc
    48721 aagaaatatc gcccggaaac cgacatggcg gatctggaca acttcgactc tgcaaaagcg
    48781 attgctgaat ctatcggcat ccacgttgag aagagctggg gtctgggccg tatcgttacc
    48841 gagatcttcg aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat
    48901 ccggcagaag tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc
    48961 tttgagttct tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg
    49021 gaagatcagg cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa
    49081 gcgatgttct acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca
    49141 ggtctgggga ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc
    49201 gacgttattc tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac
    49261 cccggcaaat gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc
    49321 ttcactattg gctgcctgca tcaccatcca cggactgaac gcccacggcg tggcatcaat
    49381 accgtgtaat acatcagcta aatcacacca ttgataatcc atcacttcat catcgttgat
    49441 ctgtaacgca ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc
    49501 actcggatcg gtggcgcggt agcgaaagtc aggatagata gattcaggag gcgtaatttc
    49561 cacgccaagc tcataacggc aacggcggat caccgcgtct tcgctgcttt ctcccagttg
    49621 tgggtgccca caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg
    49681 gcgggtaact aataattgcc ccttggcatt aaacagccaa ctggagaacg cgagatgtaa
    49741 gagggtgtct gccgtgtgtg cggcatactt ttccagcgta ccagtgggaa ctccctgtgc
    49801 attcaataaa atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat
    49861 cattatagat aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc
    49921 gttttgagcg gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag
    49981 cgactttcaa tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc
    50041 aacagcagac tgaatatagc gcgcattaaa agattcatat tattctccat gctcattttt
    50101 cttcagcttg cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga
    50161 gacggattcc ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cgttcagcac
    50221 aacggcgcta agggttgcga gcataatgcc gctatgtagc agcggttgca gtacggccgg
    50281 taacttagaa aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc
    50341 cacaatatag aggttgtaac ggttggtggt gtagttacag cgcgacagaa ttcgaatccc
    50401 tgtcgccagt accatgccga acatcaccag cccagcaccg cccagcacaa attgcggaat
    50461 ggaggcgacc agcaccgcca tttttggcac catgccaaac aggattaata taattcccga
    50521 agaaatacac acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga
    50581 aaatgacgtg tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac
    50641 acgcagcccg cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc
    50701 taggaacatc cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat
    50761 ggaaacggga tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga
    50821 agcatcatgt agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa
    50881 tacaatcccc agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag
    50941 caagataaaa attaagacag caaaggagat acctaaataa acgggattac catattgcgg
    51001 attcccttta cctccggcgg cccagtcaat acccacctga atgatgctaa gcccgataga
    51061 agtaataacc acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg
    51121 cgctaataat gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc
    51181 gatatccggg ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat
    51241 aatcaccggc aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac
    51301 gatcccgcag caaaagagat ccgagctaat gagcatcgca atagcttctt ttgagaggcc
    51361 cagtcggtca ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg
    51421 ttgcagaccg agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg
    51481 gcggtcttgc ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc
    51541 tttcgcccgc cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct
    51601 cgtggagtgc cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca
    51661 cataatcgtg gttaaaccac gaaaatgaga agagaacctg acaatgaaat tatctgtaat
    51721 tttctcatat tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt
    51781 tttatctgga tagcgctctt ttggtcggca atgttgaaat aatgaaagcg gcatagattg
    51841 aggggcaata atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata
    51901 tatgcccgtt tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg
    51961 caaatgaata agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa
    52021 attgcctgtg cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga
    52081 ccgcgtatcc acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc
    52141 aacaatgccc cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc
    52201 gtacaactgg acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt
    52261 ggcgtcgttg agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt
    52321 tccggcacgc aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat
    52381 aaagggttac agcagataaa ggtggcccgc cagcgcaaaa cggcagcagg aaaagcgtca
    52441 tcagacgctc agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca
    52501 gataaaattt cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat
    52561 ccacaacaag cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc
    52621 aactggcatt gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga
    52681 aagattattg aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc
    52741 agggtatgtc cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc
    52801 gcagtctcta tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg
    52861 cgtcccgatg tcagcaaagt tgttccccgt atcgaaaaag tggcggtgat tggcgctggg
    52921 ccagcagggt tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt
    52981 gatcgccatc cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat
    53041 aaaacggtat taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt
    53101 aactgtgaaa ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt
    53161 ttcatcggcg tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc
    53221 ggtgtcattc aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg
    53281 gagtctgaag agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt
    53341 ggcgatacga caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc
    53401 tgcgcgtatc gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg
    53461 cgcgaggaag gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa
    53521 gatgggcgtt taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat
    53581 ggtcgccgtc gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg
    53641 attatggcct ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa
    53701 ctcgataaat ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat
    53761 ctgaaaaaag tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca
    53821 atggccgcag gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg
    53881 tgatgaaatc gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag
    53941 tagcctgtgt ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc
    54001 ggctgaaggt acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa
    54061 acgccccttg tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc
    54121 agaccaattc tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa
    54181 tgatcaccat tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct
    54241 gtgaacagcg ggaagagggg ccagcctgcg ttgaatcttg cccaacacag gcgctgcagt
    54301 tgcttactga aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcggtgaga
    54361 atcctctgtg aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac
    54421 cacccgcgta aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcagctttt
    54481 gccattcgcg tgcaaagacg cggcctacga ccagtgtcac aaagcccagc ataataccgg
    54541 taacgatatt acaagtcaga acgatgaaca cggcacacac caggccagcc atagcgtcaa
    54601 taaaatcatt gaaatccagc ttcgagacgt tacttaacat cagcaaacct acgtacatca
    54661 gagcgggtgc agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca
    54721 gcaggaataa cgcccccact acggttgcgg ttaaccctgt tttacccccg gcggcggttc
    54781 ctgccgctga ttcgatataa accgctgcgg gcgctgcgcc caccaggccg gagaatattg
    54841 aacttactga gtcactggtc agggctttgc cgccgttgat gatctggttg tctttatcca
    54901 acaaattcgc ctgaccggcg acggcacgga tggtgccagt agcgtcgaac actgcggtca
    54961 tcaccaatgc cagtacactc ggaagtacgg ttggctggag tgcgcccata atatcgaggc
    55021 tgaaaatcag agacttacca tcttcgccag tcagacttgg catcgccacc agaccgtggt
    55081 atttcaccgc tgggtcaaag attaagccga taatcgaaat tgcgataatc accaacaaga
    55141 tcccgccggg tacgcggcac ttctccagac cgaagatgac cgccagcccc agcaagctca
    55201 tcatcaccgg gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt
    55261 tgataaccat acccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac
    55321 ctgtaccgtg agcgataccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta
    55381 cggaaatagc ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc
    55441 taatttgttg cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac
    55501 caatcgccat tggcaaattg gcccacaacc ccatcagcaa cgagccgaag cccgcgacca
    55561 gacaggtggc aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa
    55621 cgacgataac ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac
    55681 gaacggtact gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg
    55741 gtgcgtccgg tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa
    55801 gttttattat tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga
    55861 acggtcatcg cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc
    55921 atagcgcagc tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt
    55981 gccaggtaaa aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt
    56041 ggccaggtaa aacgcttcat atgccgagag gcgatagcct tgtaattgca atactttgta
    56101 ggcttcgttc agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat
    56161 gcccacttta actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta
    56221 aaggttggag gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc
    56281 tttttcttcg agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg
    56341 atggtaaaca tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc
    56401 atctttgttt tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg
    56461 ttgcgccatc gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata
    56521 tagcagacga ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct
    56581 ttcggcagtg tcgagcagat aatccggtgc gttgcggtcc atcatcacct taccggcaat
    56641 catacgcata ttgatatgac tggcggcttc aaacagcgca tcaacagatt gcggatgaac
    56701 agtgccaaac accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaatgc
    56761 cgacatttcg cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt
    56821 attcaaccac tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg
    56881 gatatgtgta tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat
    56941 agtgtcagga atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg
    57001 tttaatgagt aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt
    57061 atcgaccgta cgggtgacat caataaaact gcctcgtacc gctttcaacg tgtgttctcc
    57121 tgacatcatt gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga
    57181 acgttttcct gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg
    57241 ccagcacaaa tagggttttc aactagtaca taaatagagg ctggcaatat tttaaaaatt
    57301 tcgggatcgt aggagacgcc aagtcctaaa cctaaagaag tggcgacaat aagtgtttct
    57361 cggcgcttta aaccgttggt gatgattatg cgaatcccag cgatggcaat catggaaaac
    57421 atcaacgtca ttgcgcctcc cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc
    57481 atcggaaaca aaccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa
    57541 gcgacgccag tcatctgaat aaccccatta ttttgcgcaa acgtggttaa gggtaatgac
    57601 ccgacagcgg aggcgataac agaaaccaga ccgtctgcca gcacgccgcc tttcagtcgg
    57661 gactgatact cttctccctg aatgggtcgg cgggaaacca ttgccgtggc ggtgatatcg
    57721 ccgacagctt ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag
    57781 ctaaaactaa agccgtattt gaacggatgc gggatggtga ttaacggcaa attgcgcata
    57841 ctgctgaaat ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaatg
    57901 gcgatccctc ccatgcgtag caacggactg cgacagcagt taaagccgat caccacaatt
    57961 aaaaccaata aaccaacgcc gagatgttcg taattgccga atgtaccgct gcttttggct
    58021 gcaaatcctc caccaaaatc gataatgccg actttaatca ggcttaagcc gatcatcagt
    58081 accacaatac cgctgacggt aggcgtaata acccgacgta aatagggcaa gataaatgaa
    58141 gatccgacaa ccagaaatgc gccaacgaag gagacgccga gaagcgacga catgattaac
    58201 tcttcgtgaa aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat
    58261 gaaaaattga ctgactgaat tgagagtagg ccagaaccga cgatgccgta gcggtttact
    58321 tgtaaccagg taccaatacc agaggcgatc atcgccatag aaacaagata ggcagttgtt
    58381 tcagcggaaa gctgtaaggc cgcccccacg attaacgcgg gggttaccat cggaacgaaa
    58441 attgccaaca gatgggtaat ggcaccaacg agagcctgat gaaagggagg gcgatcctcc
    58501 agttcaaata taaggtcaga acctgcattg tttatatcag acatcccttc actcctttta
    58561 aagtaaattt gatcatctga attagtaaaa taaattccca cgaatttggg gcgcaggacg
    58621 ccttgagaac ggcttatctc attttcgcag tcactattta gtcatcgtcc aaacagagaa
    58681 acaggccgcc gaagcggcca tttattattt atattttttc cagcgcagtg agtattttct
    58741 ccggtgtgaa atgccattcg cgtaaccaga tgccgcatgc atcgtgaatt gcggtagcaa
    58801 tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg aacgggccga
    58861 ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca ccaattttcg
    58921 gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca taaatgatct
    58981 cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt cccaatgcta
    59041 attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc aggcggattt
    59101 cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaggcgaaat
    59161 caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt
    59221 tatgagcaat atccccgaag ctgacttcgc ctttcttgcc gcgcacgacg cccggcgttg
    59281 ccagttgaac atctgccact gcctcaccca acatttgcgc gccgtggaac agaattttct
    59341 cccgcagatt ttccgctgcc aaacgcgccg cgttacctga gaagcaagta ccggacgagg
    59401 catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct
    59461 gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc agaccagtac
    59521 caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt ttgatcatgc
    59581 agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg acgccgcgac
    59641 cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc tcgcgaccct
    59701 gacgcaggat ctcttctagt gcacagctgg cggcggaagg aacggaggtc ggcgctttac
    59761 cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga
    59821 taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc atggtgatag
    59881 cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt
    59941 agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc gacagcgccg
    60001 gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat
    60061 ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg gtgactttcg
    60121 cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aacagtaccg
    60181 gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg tcctgtttgg
    60241 aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt ttcatgccca
    60301 cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggataacca
    60361 gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac tgctgtgcct
    60421 gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat
    60481 cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa ccgatcggga
    60541 agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct tccagagtat
    60601 ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca tcttccgcca
    60661 ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca
    60721 atgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga tcgccgacgt
    60781 gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt gcgctctgac
    60841 cacccggggt atagtagata tccgggcaat tcaggtgagt aataacgtga acgacgcccg
    60901 gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac
    60961 gtaacatttt aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg
    61021 cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt
    61081 cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca
    61141 gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa ccagaaagcg
    61201 cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg gtgataatca
    61261 gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc attgcctgtt
    61321 gaaccagact taactcgttc catttgccca aagattctgc ggtacgaata tctgccttct
    61381 ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag attattgcgt
    61441 cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt cccatgttaa
    61501 acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc gcgccattta
    61561 aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacaggtc
    61621 ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca aatcttcctg
    61681 cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat tttgtttttc
    61741 gacatcatgc agacgcagtg ctttactggc gacgccatcc agcgcaattc gcataccgtc
    61801 gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat
    61861 tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta gcaggcgatc
    61921 gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc
    61981 agcatccagt gccaacagga cgggaagcag caccgactct tcctggcggg cggcgatttc
    62041 accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg
    62101 cagcgctgca ggaataaatc gcgcatcacg cagtggctgc aagcgagaca ttgcgccaat
    62161 ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta aggaaatggc
    62221 aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg cgaaccagac
    62281 ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa
    62341 aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa gagggcatct
    62401 gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt tctggcttgt
    62461 gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat cacaccattg
    62521 accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg
    62581 tctgccagca gcggcgtcgg tgagttgtaa tcgcaaatgg ttaaatcagc tttgtaaccg
    62641 gcttccagaa gtccaaattt cgcgccaaag ttgcggctca tcagttcgtt accgttagtc
    62701 agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg tttaaagaag
    62761 gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg
    62821 cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta
    62881 tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga caagtacagc
    62941 ccatgagcga ccagcgtttt gctatcgatg agatcgaatt gcgccagtcg tgccagcagg
    63001 tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata
    63061 tgcaaaccac ggcctgtggc tttcacggct tcacgtagca tctccagacc ggcatctggc
    63121 acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc ggttgctgct
    63181 ttcttcgcct catcaatctg acgggcgaag cggatgtttt cttctacacc ttcctgcaac
    63241 tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg caggccaact
    63301 tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg agaggcatgg
    63361 tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagcccgctg
    63421 taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt
    63481 gagatgaaat ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa
    63541 aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag
    63601 ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac atcattttcg
    63661 atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc ggtgacattc
    63721 ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga
    63781 catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgttg aactgacctt
    63841 cgctgtcgat gtttaacacc cagctttggt tattcagtcg tactcgtacc cggcaatctt
    63901 ccacaaggaa gcctgggttg ctgctgttat caaagtcttg cgccaggctg aaaacggtga
    63961 ttttgtcttt gtatggttta ccgttccacg gacagaactg agcgcagttg ccgcattcgt
    64021 tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagcctggg accgcaatgg
    64081 agacgttggc gcggttcggg cagacatcca cacacttgct gcaaacgtag ttacattcga
    64141 ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc accagagtga
    64201 tagagatatc gcctttacgt tgatagattt ccgccggatt gacgttgttc cagtatttat
    64261 cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg
    64321 ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt
    64381 cagtcagacg agtttcgcca ttgtggtcga cgtctggcca gccgtttttg tccagcggca
    64441 cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt
    64501 ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacggcct ttctcatcgg
    64561 gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat
    64621 tattcaggaa acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg
    64681 cgggcatctc ttgcagtgaa cgacggtaaa cgatcgttgc tttttctacg cctggaacgc
    64741 gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt
    64801 tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt gatttccaaa
    64861 cattttggtt gtcacccgcc agtttcacac cgctattttt atcagtgccg gtggcaatca
    64921 gaacatagtg gaagccctga ttttttaact gttcaacggt caaatcgggt gagcagccat
    64981 actcaaattt cacgccgtga tcggcaacaa aatcgatatc gtgctgaatt aactcagccg
    65041 gaattcggaa ctgaggaatg atatttttca ccacgccgcc cgcattggct tcgcgttcaa
    65101 acagcgtaac cggatggccc gctctggcaa ggaagtaacc tgctgccaga cccgccggac
    65161 ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt
    65221 gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg atattcagcg
    65281 cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg
    65341 taatggcggg cagagcgttg cgttggtaga tgagttccag cgcgtcggca tagcggtgtt
    65401 cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca gtaacacagg
    65461 gggcaacgta gcaatcggtc agcggcaggt cttctgccac ttcaatacgc tcttctggct
    65521 tccagtgttt ctgggtgtat tccatggtta acgcatctgc tgccagtctg ttcagtcgtt
    65581 cgacgtcaac atggtcaagt ccccaggcgt cggagccttc cagctcgcgc atgcaggcac
    65641 ttaagcgcaa atagccgcca ggtttcagca ggtcggttgc catagtaata gggcgaatac
    65701 ctgtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa gaaatgggca
    65761 gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc gggaacagcg
    65821 cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg atggtgccga
    65881 gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc
    65941 gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta
    66001 agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg tatcccagta
    66061 aggtcgggtt aagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa
    66121 tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg ccctgcacca
    66181 tactggtggg gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt
    66241 ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga tattgcgcga
    66301 atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatt ggcggttgct
    66361 taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag
    66421 aaggctggaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc
    66481 aggctttaag cagggtaaat tcggtagacc attcggtgtt aaagcactcg tcttcggcat
    66541 cgatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga
    66601 agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag
    66661 cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta
    66721 caggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat
    66781 caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc
    66841 tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg
    66901 gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg
    66961 aggcggctcg aaacttaaag gctttttgtg aatgaactca caggtgccaa atgagaatta
    67021 aaatcactcc tgaaaattat tattgtcagc ccatttatca ctttttctct tttctcaaaa
    67081 tcagtgattc c
//
LOCUS       DAZFVO020000005        65542 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFVO020000005 DAZFVO020000000
VERSION     DAZFVO020000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 65542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.005"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(61..540)
                     /gene="folK"
                     /locus_tag="ACHKNB_RS01310"
                     /old_locus_tag="pgaptmp_000262"
     CDS             complement(61..540)
                     /gene="folK"
                     /locus_tag="ACHKNB_RS01310"
                     /old_locus_tag="pgaptmp_000262"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGU4193845.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(537..1934)
                     /gene="pcnB"
                     /locus_tag="ACHKNB_RS01315"
                     /old_locus_tag="pgaptmp_000263"
     CDS             complement(537..1934)
                     /gene="pcnB"
                     /locus_tag="ACHKNB_RS01315"
                     /old_locus_tag="pgaptmp_000263"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HGU4193846.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1994..2890)
                     /gene="gluQRS"
                     /locus_tag="ACHKNB_RS01320"
                     /old_locus_tag="pgaptmp_000264"
     CDS             complement(1994..2890)
                     /gene="gluQRS"
                     /locus_tag="ACHKNB_RS01320"
                     /old_locus_tag="pgaptmp_000264"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HGU4193847.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEPGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2957..3412)
                     /gene="dksA"
                     /locus_tag="ACHKNB_RS01325"
                     /old_locus_tag="pgaptmp_000265"
     CDS             complement(2957..3412)
                     /gene="dksA"
                     /locus_tag="ACHKNB_RS01325"
                     /old_locus_tag="pgaptmp_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HGU4193848.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3590..4294)
                     /gene="sfsA"
                     /locus_tag="ACHKNB_RS01330"
                     /old_locus_tag="pgaptmp_000266"
     CDS             complement(3590..4294)
                     /gene="sfsA"
                     /locus_tag="ACHKNB_RS01330"
                     /old_locus_tag="pgaptmp_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HGU4193849.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAELSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4309..4839)
                     /gene="thpR"
                     /locus_tag="ACHKNB_RS01335"
                     /old_locus_tag="pgaptmp_000267"
     CDS             complement(4309..4839)
                     /gene="thpR"
                     /locus_tag="ACHKNB_RS01335"
                     /old_locus_tag="pgaptmp_000267"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HGU4193850.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4913..7342
                     /gene="hrpB"
                     /locus_tag="ACHKNB_RS01340"
                     /old_locus_tag="pgaptmp_000268"
     CDS             4913..7342
                     /gene="hrpB"
                     /locus_tag="ACHKNB_RS01340"
                     /old_locus_tag="pgaptmp_000268"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HGU4193851.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7538..10072
                     /gene="mrcB"
                     /locus_tag="ACHKNB_RS01345"
                     /old_locus_tag="pgaptmp_000269"
     CDS             7538..10072
                     /gene="mrcB"
                     /locus_tag="ACHKNB_RS01345"
                     /old_locus_tag="pgaptmp_000269"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HGU4193852.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10292..12535
                     /gene="fhuA"
                     /locus_tag="ACHKNB_RS01350"
                     /old_locus_tag="pgaptmp_000270"
     CDS             10292..12535
                     /gene="fhuA"
                     /locus_tag="ACHKNB_RS01350"
                     /old_locus_tag="pgaptmp_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HGU4193853.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNDGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12586..13383
                     /gene="fhuC"
                     /locus_tag="ACHKNB_RS01355"
                     /old_locus_tag="pgaptmp_000271"
     CDS             12586..13383
                     /gene="fhuC"
                     /locus_tag="ACHKNB_RS01355"
                     /old_locus_tag="pgaptmp_000271"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HGU4193854.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13383..14273
                     /gene="fhuD"
                     /locus_tag="ACHKNB_RS01360"
                     /old_locus_tag="pgaptmp_000272"
     CDS             13383..14273
                     /gene="fhuD"
                     /locus_tag="ACHKNB_RS01360"
                     /old_locus_tag="pgaptmp_000272"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HGU4193855.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPLLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14270..16252
                     /gene="fhuB"
                     /locus_tag="ACHKNB_RS01365"
                     /old_locus_tag="pgaptmp_000273"
     CDS             14270..16252
                     /gene="fhuB"
                     /locus_tag="ACHKNB_RS01365"
                     /old_locus_tag="pgaptmp_000273"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HGU4193856.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQATWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGID
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQIR"
     gene            complement(16287..17567)
                     /gene="hemL"
                     /locus_tag="ACHKNB_RS01370"
                     /old_locus_tag="pgaptmp_000274"
     CDS             complement(16287..17567)
                     /gene="hemL"
                     /locus_tag="ACHKNB_RS01370"
                     /old_locus_tag="pgaptmp_000274"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HGU4193857.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17733..17780
                     /gene="yadX"
                     /locus_tag="ACHKNB_RS01375"
                     /old_locus_tag="pgaptmp_000275"
     CDS             17733..17780
                     /gene="yadX"
                     /locus_tag="ACHKNB_RS01375"
                     /old_locus_tag="pgaptmp_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HGU4193858.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17792..19213
                     /gene="clcA"
                     /locus_tag="ACHKNB_RS01380"
                     /old_locus_tag="pgaptmp_000276"
     CDS             17792..19213
                     /gene="clcA"
                     /locus_tag="ACHKNB_RS01380"
                     /old_locus_tag="pgaptmp_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HGU4193859.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19237..19302
                     /gene="yadW"
                     /locus_tag="ACHKNB_RS01385"
                     /old_locus_tag="pgaptmp_000277"
     CDS             19237..19302
                     /gene="yadW"
                     /locus_tag="ACHKNB_RS01385"
                     /old_locus_tag="pgaptmp_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HGU4193860.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19295..19639
                     /gene="erpA"
                     /locus_tag="ACHKNB_RS01390"
                     /old_locus_tag="pgaptmp_000278"
     CDS             19295..19639
                     /gene="erpA"
                     /locus_tag="ACHKNB_RS01390"
                     /old_locus_tag="pgaptmp_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HGU4193861.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19686..20309)
                     /gene="yadS"
                     /locus_tag="ACHKNB_RS01395"
                     /old_locus_tag="pgaptmp_000279"
     CDS             complement(19686..20309)
                     /gene="yadS"
                     /locus_tag="ACHKNB_RS01395"
                     /old_locus_tag="pgaptmp_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HGU4193862.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20347..21147)
                     /gene="btuF"
                     /locus_tag="ACHKNB_RS01400"
                     /old_locus_tag="pgaptmp_000280"
     CDS             complement(20347..21147)
                     /gene="btuF"
                     /locus_tag="ACHKNB_RS01400"
                     /old_locus_tag="pgaptmp_000280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HGU4193863.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLQDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="ACHKNB_RS01405"
                     /old_locus_tag="pgaptmp_000281"
     CDS             complement(21140..21838)
                     /gene="mtnN"
                     /locus_tag="ACHKNB_RS01405"
                     /old_locus_tag="pgaptmp_000281"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGU4193864.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21922..23439
                     /gene="dgt"
                     /locus_tag="ACHKNB_RS01410"
                     /old_locus_tag="pgaptmp_000282"
     CDS             21922..23439
                     /gene="dgt"
                     /locus_tag="ACHKNB_RS01410"
                     /old_locus_tag="pgaptmp_000282"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HGU4193865.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23569..24993
                     /gene="degP"
                     /locus_tag="ACHKNB_RS01415"
                     /old_locus_tag="pgaptmp_000283"
     CDS             23569..24993
                     /gene="degP"
                     /locus_tag="ACHKNB_RS01415"
                     /old_locus_tag="pgaptmp_000283"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HGU4193866.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25148..26305
                     /gene="cdaR"
                     /locus_tag="ACHKNB_RS01420"
                     /old_locus_tag="pgaptmp_000284"
     CDS             25148..26305
                     /gene="cdaR"
                     /locus_tag="ACHKNB_RS01420"
                     /old_locus_tag="pgaptmp_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HGU4193867.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26394..26780)
                     /gene="yaeH"
                     /locus_tag="ACHKNB_RS01425"
                     /old_locus_tag="pgaptmp_000285"
     CDS             complement(26394..26780)
                     /gene="yaeH"
                     /locus_tag="ACHKNB_RS01425"
                     /old_locus_tag="pgaptmp_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HGU4193868.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27095..27919)
                     /gene="dapD"
                     /locus_tag="ACHKNB_RS01430"
                     /old_locus_tag="pgaptmp_000286"
     CDS             complement(27095..27919)
                     /gene="dapD"
                     /locus_tag="ACHKNB_RS01430"
                     /old_locus_tag="pgaptmp_000286"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HGU4193869.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27950..30622)
                     /gene="glnD"
                     /locus_tag="ACHKNB_RS01435"
                     /old_locus_tag="pgaptmp_000287"
     CDS             complement(27950..30622)
                     /gene="glnD"
                     /locus_tag="ACHKNB_RS01435"
                     /old_locus_tag="pgaptmp_000287"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HGU4193870.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30684..31478)
                     /gene="map"
                     /locus_tag="ACHKNB_RS01440"
                     /old_locus_tag="pgaptmp_000288"
     CDS             complement(30684..31478)
                     /gene="map"
                     /locus_tag="ACHKNB_RS01440"
                     /old_locus_tag="pgaptmp_000288"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HGU4193871.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31846..32571
                     /gene="rpsB"
                     /locus_tag="ACHKNB_RS01445"
                     /old_locus_tag="pgaptmp_000289"
     CDS             31846..32571
                     /gene="rpsB"
                     /locus_tag="ACHKNB_RS01445"
                     /old_locus_tag="pgaptmp_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGU4193872.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32829..33680
                     /gene="tsf"
                     /locus_tag="ACHKNB_RS01450"
                     /old_locus_tag="pgaptmp_000290"
     CDS             32829..33680
                     /gene="tsf"
                     /locus_tag="ACHKNB_RS01450"
                     /old_locus_tag="pgaptmp_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGU4193873.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33827..34552
                     /gene="pyrH"
                     /locus_tag="ACHKNB_RS01455"
                     /old_locus_tag="pgaptmp_000291"
     CDS             33827..34552
                     /gene="pyrH"
                     /locus_tag="ACHKNB_RS01455"
                     /old_locus_tag="pgaptmp_000291"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGU4193874.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34844..35401
                     /gene="frr"
                     /locus_tag="ACHKNB_RS01460"
                     /old_locus_tag="pgaptmp_000292"
     CDS             34844..35401
                     /gene="frr"
                     /locus_tag="ACHKNB_RS01460"
                     /old_locus_tag="pgaptmp_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGU4193875.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35493..36689
                     /gene="ispC"
                     /locus_tag="ACHKNB_RS01465"
                     /old_locus_tag="pgaptmp_000293"
     CDS             35493..36689
                     /gene="ispC"
                     /locus_tag="ACHKNB_RS01465"
                     /old_locus_tag="pgaptmp_000293"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HGU4193876.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36878..37636
                     /gene="ispU"
                     /locus_tag="ACHKNB_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /gene_synonym="uppS"
     CDS             36878..37636
                     /gene="ispU"
                     /locus_tag="ACHKNB_RS01470"
                     /old_locus_tag="pgaptmp_000294"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HGU4193877.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37649..38506
                     /gene="cdsA"
                     /locus_tag="ACHKNB_RS01475"
                     /old_locus_tag="pgaptmp_000295"
     CDS             37649..38506
                     /gene="cdsA"
                     /locus_tag="ACHKNB_RS01475"
                     /old_locus_tag="pgaptmp_000295"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4193878.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38518..39870
                     /gene="rseP"
                     /locus_tag="ACHKNB_RS01480"
                     /old_locus_tag="pgaptmp_000296"
     CDS             38518..39870
                     /gene="rseP"
                     /locus_tag="ACHKNB_RS01480"
                     /old_locus_tag="pgaptmp_000296"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HGU4193879.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39900..42332
                     /gene="bamA"
                     /locus_tag="ACHKNB_RS01485"
                     /old_locus_tag="pgaptmp_000297"
     CDS             39900..42332
                     /gene="bamA"
                     /locus_tag="ACHKNB_RS01485"
                     /old_locus_tag="pgaptmp_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HGU4193880.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42454..42939
                     /gene="skp"
                     /locus_tag="ACHKNB_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42454..42939
                     /gene="skp"
                     /locus_tag="ACHKNB_RS01490"
                     /old_locus_tag="pgaptmp_000298"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HGU4193881.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42943..43968
                     /gene="lpxD"
                     /locus_tag="ACHKNB_RS01495"
                     /old_locus_tag="pgaptmp_000299"
     CDS             42943..43968
                     /gene="lpxD"
                     /locus_tag="ACHKNB_RS01495"
                     /old_locus_tag="pgaptmp_000299"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HGU4193882.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44073..44528
                     /gene="fabZ"
                     /locus_tag="ACHKNB_RS01500"
                     /old_locus_tag="pgaptmp_000300"
     CDS             44073..44528
                     /gene="fabZ"
                     /locus_tag="ACHKNB_RS01500"
                     /old_locus_tag="pgaptmp_000300"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGU4193883.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44532..45320
                     /gene="lpxA"
                     /locus_tag="ACHKNB_RS01505"
                     /old_locus_tag="pgaptmp_000301"
     CDS             44532..45320
                     /gene="lpxA"
                     /locus_tag="ACHKNB_RS01505"
                     /old_locus_tag="pgaptmp_000301"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HGU4193884.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45320..46468
                     /gene="lpxB"
                     /locus_tag="ACHKNB_RS01510"
                     /old_locus_tag="pgaptmp_000302"
     CDS             45320..46468
                     /gene="lpxB"
                     /locus_tag="ACHKNB_RS01510"
                     /old_locus_tag="pgaptmp_000302"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HGU4193885.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46465..47061
                     /gene="rnhB"
                     /locus_tag="ACHKNB_RS01515"
                     /old_locus_tag="pgaptmp_000303"
     CDS             46465..47061
                     /gene="rnhB"
                     /locus_tag="ACHKNB_RS01515"
                     /old_locus_tag="pgaptmp_000303"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGU4193886.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47098..50580
                     /gene="dnaE"
                     /locus_tag="ACHKNB_RS01520"
                     /old_locus_tag="pgaptmp_000304"
     CDS             47098..50580
                     /gene="dnaE"
                     /locus_tag="ACHKNB_RS01520"
                     /old_locus_tag="pgaptmp_000304"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGU4193887.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50593..51552
                     /gene="accA"
                     /locus_tag="ACHKNB_RS01525"
                     /old_locus_tag="pgaptmp_000305"
     CDS             50593..51552
                     /gene="accA"
                     /locus_tag="ACHKNB_RS01525"
                     /old_locus_tag="pgaptmp_000305"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGU4193888.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51651..53792
                     /gene="ldcC"
                     /locus_tag="ACHKNB_RS01530"
                     /old_locus_tag="pgaptmp_000306"
     CDS             51651..53792
                     /gene="ldcC"
                     /locus_tag="ACHKNB_RS01530"
                     /old_locus_tag="pgaptmp_000306"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HGU4193889.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53849..54238
                     /gene="yaeR"
                     /locus_tag="ACHKNB_RS01535"
                     /old_locus_tag="pgaptmp_000307"
     CDS             53849..54238
                     /gene="yaeR"
                     /locus_tag="ACHKNB_RS01535"
                     /old_locus_tag="pgaptmp_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4193890.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54303..55601
                     /gene="tilS"
                     /locus_tag="ACHKNB_RS01540"
                     /old_locus_tag="pgaptmp_000308"
     CDS             54303..55601
                     /gene="tilS"
                     /locus_tag="ACHKNB_RS01540"
                     /old_locus_tag="pgaptmp_000308"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGU4193891.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55650..55910)
                     /gene="rof"
                     /locus_tag="ACHKNB_RS01545"
                     /old_locus_tag="pgaptmp_000309"
     CDS             complement(55650..55910)
                     /gene="rof"
                     /locus_tag="ACHKNB_RS01545"
                     /old_locus_tag="pgaptmp_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HGU4193892.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55897..56097)
                     /gene="yaeP"
                     /locus_tag="ACHKNB_RS01550"
                     /old_locus_tag="pgaptmp_000310"
     CDS             complement(55897..56097)
                     /gene="yaeP"
                     /locus_tag="ACHKNB_RS01550"
                     /old_locus_tag="pgaptmp_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HGU4193893.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56263..56808
                     /gene="yaeQ"
                     /locus_tag="ACHKNB_RS01555"
                     /old_locus_tag="pgaptmp_000311"
     CDS             56263..56808
                     /gene="yaeQ"
                     /locus_tag="ACHKNB_RS01555"
                     /old_locus_tag="pgaptmp_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HGU4193894.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56805..57227
                     /gene="arfB"
                     /locus_tag="ACHKNB_RS01560"
                     /old_locus_tag="pgaptmp_000312"
     CDS             56805..57227
                     /gene="arfB"
                     /locus_tag="ACHKNB_RS01560"
                     /old_locus_tag="pgaptmp_000312"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HGU4193895.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57241..57951
                     /gene="nlpE"
                     /locus_tag="ACHKNB_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /gene_synonym="cutF"
     CDS             57241..57951
                     /gene="nlpE"
                     /locus_tag="ACHKNB_RS01565"
                     /old_locus_tag="pgaptmp_000313"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HGU4193896.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58106..58929)
                     /gene="yaeF"
                     /locus_tag="ACHKNB_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /pseudo
     CDS             complement(58106..58929)
                     /gene="yaeF"
                     /locus_tag="ACHKNB_RS01570"
                     /old_locus_tag="pgaptmp_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58983..60701)
                     /gene="proS"
                     /locus_tag="ACHKNB_RS01575"
                     /old_locus_tag="pgaptmp_000315"
     CDS             complement(58983..60701)
                     /gene="proS"
                     /locus_tag="ACHKNB_RS01575"
                     /old_locus_tag="pgaptmp_000315"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGU4193897.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60813..61520)
                     /gene="tsaA"
                     /locus_tag="ACHKNB_RS01580"
                     /old_locus_tag="pgaptmp_000316"
     CDS             complement(60813..61520)
                     /gene="tsaA"
                     /locus_tag="ACHKNB_RS01580"
                     /old_locus_tag="pgaptmp_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HGU4193898.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDVGFEVFALEPR"
     gene            complement(61517..61921)
                     /gene="rcsF"
                     /locus_tag="ACHKNB_RS01585"
                     /old_locus_tag="pgaptmp_000317"
     CDS             complement(61517..61921)
                     /gene="rcsF"
                     /locus_tag="ACHKNB_RS01585"
                     /old_locus_tag="pgaptmp_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HGU4193899.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62039..62854)
                     /gene="metQ"
                     /locus_tag="ACHKNB_RS01590"
                     /old_locus_tag="pgaptmp_000318"
     CDS             complement(62039..62854)
                     /gene="metQ"
                     /locus_tag="ACHKNB_RS01590"
                     /old_locus_tag="pgaptmp_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HGU4193900.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62894..63547)
                     /gene="metI"
                     /locus_tag="ACHKNB_RS01595"
                     /old_locus_tag="pgaptmp_000319"
     CDS             complement(62894..63547)
                     /gene="metI"
                     /locus_tag="ACHKNB_RS01595"
                     /old_locus_tag="pgaptmp_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HGU4193901.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63540..64571)
                     /gene="metN"
                     /locus_tag="ACHKNB_RS01600"
                     /old_locus_tag="pgaptmp_000320"
     CDS             complement(63540..64571)
                     /gene="metN"
                     /locus_tag="ACHKNB_RS01600"
                     /old_locus_tag="pgaptmp_000320"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HGU4193902.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64759..65334
                     /gene="gmhB"
                     /locus_tag="ACHKNB_RS01605"
                     /old_locus_tag="pgaptmp_000321"
     CDS             64759..65334
                     /gene="gmhB"
                     /locus_tag="ACHKNB_RS01605"
                     /old_locus_tag="pgaptmp_000321"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HGU4193903.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15461 a        16333 c        18077 g        15671 t
ORIGIN      
        1 attaaggaaa gagaggatgt atgattttca gaaaatattt atattcgtaa tataaataaa
       61 ttaccatttg cttaatttgt caaatgctct tgtatgtaag acttcacgca acgtctcccc
      121 atcaggaaac gccagctccg gtgcaatctc aaacagcggc cacagcataa atccacgatt
      181 cttcatatcg tagtgcggaa cggtcaggcg ttcggtattt atcacttcat taccaaacag
      241 catgatatcg agatccagcg tgcgtggtcc ccagcgttca gctttacgaa cacgaccctg
      301 ttgcagttct atgcgctgtg tatggttgag taactcttcc ggggcgagcg aagtttcaag
      361 cgccacggcg gcgttgaggt aatcgggttg atcttgcggc cccagcggtg gggtgcggta
      421 aaacgaagaa acggcaagaa tgtggctttc agggatatcg cctaatgctt tcagggcagc
      481 attgacctgc tccagcggag aggccagatt gctgcctatg gcaatatacg ccactgtcat
      541 gcggtaccct cacgacgcgg tgcgcgtttg cgtggacgac gagtacggcg acgcggtgac
      601 ggctcttcat ccaactcgtt aagcatccct ttttgatctg gtggcgcgga aacctggaac
      661 tcaccccacc atttcaccag acgctgcagt tcagcgttac gttcaacttc agctcgcaag
      721 gccaacaggt cataagccgc acggaactta ggatgctcca gcagtttcca tgcgcgttta
      781 ccctgacgac gggacatacg caactgcaac tgccagatat cgcgggttaa cgtcgtcaga
      841 cgtttcggga ttgccagtga acggcaggct tcgtccagca cgtcgttcat cgccagcgcg
      901 aaagcgtcgt gataggtcag gccgctttcc tgggcaatct tctgtgccgt ctccagcagt
      961 gggtaccaga acatggcggc aaacaggaac gccgggttca cgcgcatatc gttatggata
     1021 cgcgtatcgg tattcttcag cacctgttca atgatccgct ccatcgggct gtcgccattt
     1081 tccgtgaagt agcgggtaat ggtcgggaac agcggctgga acagatgata ttcacacaac
     1141 agcttatagg tatcgtaacc gtagcccgct tgtagcagtt taagcgattc ttcaaacagg
     1201 cgtgccggtg ggatatcgtt caacagggta gcgaggcgag ggatcggttc tgcggtttcc
     1261 gggctgatgc gcatacccaa tttggcggca aaacgtaccg cgcgcagcat acgtaccgga
     1321 tcttcacggt agcgcgtttc cgggttacca atcagacgga taacgccgtc cttcagatcc
     1381 ttcataccgc caacgtaatc acggacggta aaatccgcta cgctgtaata gaggctgttg
     1441 atagtgaaat cgcggcgctg ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc
     1501 aacatgccgt tttgcccgcg ttgggaggtc gtgcggtcgc tgacgttacc ttcgtggtgt
     1561 ccacggaagg tcgcaacttc gataatctcc gggccaaaca ttacatgagc cagacggaaa
     1621 cggcgaccca ccaggcggca gttacggaac agtttgcgca cctgctcagg cgtggcgtta
     1681 gtggttacgt caaaatcttt cggctttttg ccaagtaaca ggtcgcgcac gccgccgcca
     1741 accagccagg cttcgtatcc cgctttgttg agcctgtaca ttaccttcag ggcattttca
     1801 ctgatatctt tgcgggaaat agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg
     1861 gcgactgcct gttcagcctc gctttcctcg cggcttagca ccttgcggca aaaattagcg
     1921 actcgggtaa aaatagtaca cctcggtagt gtcaaacatc attcaggaca aaaaaatagc
     1981 ggctaatcat agctcagcat gacgcatttg agaatgttga atttacaatt gccgactcgg
     2041 gcacggcggt aagccgccag tttttgacgg ctgactgaag gatttgctcg acgctgaaat
     2101 cctgccagtg tgcttctgcc tgctgcccca gaaattgaag tgccgcgatt agtaccgggc
     2161 gtggatcgcc tttcggcaac gcaggcgcat gattctgctt ggaaagttta gcgccttgtg
     2221 gattaagcgc cagcggcaga tgaatgtaat ctggcacttt ccagccaaaa agctggtaca
     2281 gcgagatttg ccttactgtt ggttcaatca gatcagcccc acgcactatt tctgtaacgc
     2341 cctggaaatg atcatcaacc acaacagcca ggttgtaggc gaacaaccca tcacggcgat
     2401 gaatgataaa atcttcccgt gccagttttt cgtcggcgtg aataatgccg cgcagctggt
     2461 cagtaaattg cgtgaccgga tgctgctggc ggatacgcac tgcggcattg tctggtccat
     2521 gatgcaacac ccggcaatga ccgtcgtaaa taccgccaat gctttgaata cgcgcacgcg
     2581 tacaggtgca gtaataactt agtcctggtt catgtaacca ggcgagtgct tcacgatagg
     2641 cgtggtgacg ttgcgattgc cagagaacat cgccgtccca gtgcagaccg taatgttcca
     2701 gctggcgcag gatagtttct gcggcaccgg gaacttcacg aggcgggtcg atatcttcta
     2761 tgcgtaccag ccagcgacct tgccgggcgc gagcctgcaa atagctgccg agcgcggcga
     2821 tcagagagcc aaaatgaagc tcgccggaag gagagggggc gaagcggcca atatactgtg
     2881 tgtctgtcat ctctttgaac aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt
     2941 gatggaacgg ctgtaattag ccagccatct gtttttcgcg aatttcagcc agcgttttgc
     3001 agtcgatgca cagatcggct gtcgggcgcg cttccagacg gcgaatacca atttcaacac
     3061 cgcaggattc gcagtagccg aaatcttcgt cttccacttt tttcagcgtc ttctcgatct
     3121 ttttgatcag cttacgctcg cgatcgcggt tacgcagttc gaggctgaac tcttcttcct
     3181 gggctgcacg gtctaccggg tccgggaagt tggctgcttc atcctgcata tgtgtaacgg
     3241 tgcgatcgac ttcatccctg agttgattac gccatgcttc cagaatacga cggaagtgcg
     3301 ccagctgggc ttcattcata tactcttcgc ccggcttctc ctgatatggt tccaccccag
     3361 cgatggcgag aatactcagg gacgatgttt tacggttttg cccttcttgc atgttgcttc
     3421 tccttaacac gcactatcga tccccatgtt cgggggaaaa atgaggccgc tataaatagc
     3481 agatgctttt ccggatagca attatctaaa cgtaacactt gacaactgtg tgaggaaaag
     3541 cgtatttgcg cacgcgacca gaatgtaaat taaccagtta cttactttac tacaatgtaa
     3601 ccggcagtga ttttttaaga gccatgcctt cagcagaaat ttccgctttg taagccagaa
     3661 tttctacccc cctctgttga gcttctgaca atagttgcgc gtatttctca tcgatgtggc
     3721 gcgcgggtga aaaccgtgta atggctgaat gcagcacggc gaaaaagata accgcacgct
     3781 ggccttcagc cgctacgctc atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg
     3841 catcgggaaa atatccctgt tcgttctccg ctaacgtaac cgatttcact tcaatatagc
     3901 agtctggacg cgaatccgcc tgcaacataa agtcaatacg gctgagttct gcgccgtatt
     3961 ttacttcgct tttcagcgag ctatagcctg acagttctga aattgattca ttaaggatag
     4021 cctctttcgt caacctgtta gcccaaagcg tgttgacgca aataaatgcg ccgctctggc
     4081 tttgagttaa ttcccaggtg tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc
     4141 agacggtatc gccaggcgtt gcacaaccgg tcattgcacc cgtattcggg cagtgtagcg
     4201 ttaattcgcg accatcgggt gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta
     4261 gcgtcgcgcg ctgtagaggg ggagagaatt ccattgcgac aatccttgtt attgcgttag
     4321 cgcccagcgt tttagcggcg tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag
     4381 ggtgaactcc gtcaccgcat aagaccagtt aaaacctggc ggcgggattg tcaccgcctc
     4441 gctggcgtcg cgcaataagg taatatgtgg atgaaacgga cgattgcttt gaaaacaacc
     4501 gctgcgggca gcctgtgaac ggagcatatt cgccagctgg attaagccgc gtggaggctg
     4561 acgcatccct aaccacacca cacgcgaacg cagccattgt ccggcgtcat caagcgtgag
     4621 tgtgaaacca ggttgacgaa tccgtccggc taaaagagaa agcgccttct ctttctcggc
     4681 gctcacttcg cctaaaaatg ccagagtcag atgcaaatta tcggcggcga ccggacgtcc
     4741 cgcctcaggt gggaagtgtg tggcgcgcca atggataatc tgttcgcgga tttctgcagg
     4801 taagtcgata gcaaagaaca gacgttgcgg ttcagacatg tgaggcactc ggttatgaat
     4861 taccgcgatg ctacaatgtg gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc
     4921 gttgcccgtt gctgccgtct tatctgaatt acttaccgct ctcgattgtg cgccgcaggt
     4981 attattaagt gcgccgaccg gggccgggaa atcaacctgg ctgccgctgc aactgctggc
     5041 gcatcccggc attaacggga aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa
     5101 cgtcgcgcaa cggctggcgg agctgcttaa cgaaaagcca ggcgataccg ttggctaccg
     5161 gatgcgtgcg caaaactgcg tcgggccgaa tacccgcctg gaagtggtta ccgaaggcgt
     5221 gctgacgcgc atgatccagc gtgacccgga actgagcggt gttggactgg tgatccttga
     5281 tgaatttcat gagcgcagct tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca
     5341 acaaggtctg cgtgatgacc ttaaactgct gattatgtcg gctaccctgg acaacgaccg
     5401 cttgcagcaa atgctgccag aagcgcctgt cgtcatctca gaagggcgct cgtttccggt
     5461 tgaacgccgt tatttaccgc tgcccgcgca tcagcgtttt gacgatgccg ttgcggtagc
     5521 caccgctgaa atgctgcgtc aggaaagcgg atcattactg ttatttttac ctggcgtcgg
     5581 agaaattcag cgtgtgcagg aacaactggc ttcgcgcatc ggcagtgatg tattgctctg
     5641 cccgctgtat ggcgcgttgt cgctgaacga tcagcgaaaa gcgatcctcc cggcaccgca
     5701 agggatgcgc aaagtggtgc tggcgaccaa tattgctgaa accagtttaa ccattgaagg
     5761 tattcgtctg gtggtggatt gtgcccagga gcgtgtggcg cgttttgatc cgcgcacggg
     5821 gcttacgcga ctgattactc aacgcgttag ccaggcatcc atgacgcagc gtgccgggcg
     5881 cgccgggcgt ctggagccgg gtatctgcct gcatttaatc gccaaagaac aagcagaacg
     5941 cgccgcggcg caaagtgaac cggagatctt acaaagcgat ctttccggtt tgctgatgga
     6001 attactgcaa tggggatgca gcgatccggc gcagatgagc tggctggatc aaccgccagt
     6061 agtgaatcta ctggccgcga aacgtctgtt acaaatgctg ggggcactgg agggtgaacg
     6121 gcttagtgcg caagggcaaa aaatggcagc gctgggtaac gatccgcgtt tagcggcaat
     6181 gctggttagc gcgaagaacg acgacgaagc tgctaccgcg gcaaaaattg ccgccattct
     6241 cgaagagccg ccacggatgg gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc
     6301 agcctggcag caacgtagtc agcaactgtt aaaacgctta aacgtacgtg gcggtgaggc
     6361 agacagttcg cttatcgcgc cgctacttgc cggggcgttt gccgatcgca ttgctcgtcg
     6421 ccgtgggcaa gatggacgct atcaactggc aaacggcatg ggagcgatgc tcgatgccaa
     6481 cgacgcgcta agccgccacg aatggttgat cgcaccgtta ttattgcagg gcagcgcctc
     6541 gccggatgcg cggattttac tggcgctgcc ggtcgatatt gatgagttag tacaacgctg
     6601 cccgcagctg gtacagcagt ctgacactgt ggagtgggat gacgcgcaag gtacgctgaa
     6661 agcctggcgt cggctacaaa tcggtcagtt gacggtgaaa gtgcagccgc tggcgaaacc
     6721 gtcagaagac gagttgcatc aggcgatgct taatggcatc cgtgataaag gtttaagcgt
     6781 gctcaactgg acggcggaag cggaacagct acgcttgcgt ttgttatgcg ccgcaaagtg
     6841 gttgccggaa tatgactggc cagcggttga tgatgaaagt ttattggcag cgctggaaac
     6901 gtggctgctg ccacatatga ctggcgtaca ttcactacgc ggcctgaaat cactcgacat
     6961 ttatcaggca ctacgcggat tacttgattg gggaatgcag caacgtctgg atagtgaatt
     7021 gcctgcgcat tacactgtgc cgacgggaag ccggatcgcc attcgttatc atgaagataa
     7081 cccgcccgcg ctggcggtga gaatgcaaga gatgtttggc gaggccacca atccgacgat
     7141 cgcccagggg cgcgtgccgc tggtgctgga gttgctttca cctgcccaaa ggccattaca
     7201 aatcacacga gatttgagcg ccttctggaa aggagcgtac cgtgaggtgc aaaaagagat
     7261 gaaagggcgt tatcccaaac atgtctggcc ggacgacccg gcaaatactg caccgacgcg
     7321 acggacgaaa aagtattcgt aaggttggtt ttctccctct ccctgtggga gagggccggg
     7381 gtgagggcat cagcgcgcac gttcaccctc accctaaccc tctccctcaa gggagagggg
     7441 accgatcgag cacaaatttt gagagatatc ttcttctgtc ttgtaacaga agaacagaaa
     7501 atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg gccgggaatg accgcgagcc
     7561 aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa aaggtaagcc gtcgtcgtta
     7621 cgaagatgac gatgattacg acgattatga tgactatgag gatgaagaac cgatgccgcg
     7681 caaaggtaag ggcaaaggca aagggcgtaa gcctcgtggc aaacgcggct ggctatggct
     7741 actgctaaaa ctggctatcg tttttgccgt gctgatcgcc atttacggcg tttatctcga
     7801 tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa ctgcctgcgg cagtttatgg
     7861 ccgaatggtc aatcttgagc cagacatgac catcagcaag aacgagatgg tgaagctgct
     7921 ggaggcgacc cagtatcgtc aggtgtcgaa aatgacccgt cctggcgaat ttaccgtgca
     7981 ggccaacagc attgagatga ttcgccgtcc gtttgatttc ccggacagta aagaaggaca
     8041 ggtgcgcgcg cgtctgacct ttgatggcga tcatctggcg acgatcgtca atatggagaa
     8101 caaccgtcag ttcggtttct tccgtcttga tccgcgtctg atcaccatga tctcttcgcc
     8161 aaacggtgag cagcgtctgt ttgtgccgcg cagtggtttc ccggatttgc tggtggatac
     8221 tttgctggcg acagaagacc gtcattttta cgagcatgat ggaatcagtc tctactcaat
     8281 cggacgtgcg gtgctggcaa acctgaccgc cggacgcacg gtacagggtg cgagtacgct
     8341 gacgcaacag ctggtgaaaa acctgttcct ctccagcgag cgttcttact ggcgtaaagc
     8401 gaacgaagct tacatggcgc tgatcatgga cgcgcgttac agcaaagacc gtattcttga
     8461 gctgtatatg aacgaggtgt atctcggtca gagcggcgac aacgaaatcc gcggcttccc
     8521 gctggcaagc ttgtattact ttggtcgccc ggtagaagag ctaagcctcg accagcaggc
     8581 gctgttagtc ggtatggtga aaggggcgtc catctacaac ccgtggcgta acccaaaact
     8641 ggcgctggag cgacgtaatc tggtgctgcg tctgctgcaa cagcaacaga ttattgatca
     8701 agaactctat gacatgttga gtgcccgtcc gctgggggtt cagccgcgcg gtggggtgat
     8761 ctctcctcag ccagccttta tgcaactggt gcgtcaggag ctgcaggcaa aactgggcga
     8821 taaggtaaaa gatctctccg gcgtgaagat cttcactacc tttgactcgg tggcccagga
     8881 cgcggcagaa aaagccgccg tggaaggcat tccggcactg aagaaacagc gtaagttgag
     8941 cgatcttgaa actgcgattg tggtcgtcga ccgctttagt ggtgaagttc gtgcgatggt
     9001 cggaggttct gagccgcagt ttgcgggcta caaccgtgcg atgcaggcgc gtcgttcgat
     9061 tggttccctt gcaaaaccag cgacttatct gacggcttta agccagccga aaatctatcg
     9121 cctgaacacg tggattgccg atgcgccaat cgcgctgcgt cagccgaatg gccaggtctg
     9181 gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc agagtgatgc tggtggatgc
     9241 gttgacccgt tcgatgaacg tgccgacggt aaatctgggg atggcgctgg ggctgcctgc
     9301 agttacggat acctggatca aactgggcgt accgaaagat cagctgcatc cggttccggc
     9361 gatgctgctg ggggcgttga acttaacgcc aatcgaagtg gcgcaggcgt tccagaccat
     9421 cgccagcggt ggtaaccgtg caccgctttc tgcactgcgt tcggtaatcg cggaagatgg
     9481 caaggtgctg tatcagagct tcccgcaggc agaacgcgct gttccggcgc aggcggcgta
     9541 tctgacacta tggaccatgc agcaggtggt acaacgtggt acgggtcgtc agcttggggc
     9601 gaaatacccg aacctgcatc tggcagggaa aacagggact accaacaata acgtagatac
     9661 ctggtttgca ggtattgacg gcagcacggt gaccatcacc tgggtaggcc gtgataacaa
     9721 ccagccgacc aaactgtatg gagccagcgg ggcaatgtcg atttatcagc gttatctggc
     9781 taaccagacg ccaacgccgc tgaatcttgt tccgccagaa gatattgcag atatgggcgt
     9841 ggactacgac ggcaactttg tttgcagcgg tggcatgcgt gtcttgccgg tctggaccag
     9901 cgatccgcaa tcgctgtgcc agcagagcga gatgcagcag cagccgtcag gcaatccgtt
     9961 tgatcagtct tctcagccgc agcaacagcc gcaacagcaa cctgctcagc aagagcagaa
    10021 agacagcgac ggtgtagccg gttggatcaa ggatatgttt ggtagtaatt aacatctaag
    10081 cgtgaaatac cggatggcga gttgccatcc ggtaaaataa catcccatct aagatattaa
    10141 ccctttcttt tcatctggtt gtttattaac ccttcaggaa cgctcagatt gcgtaccgct
    10201 tgcgaacccg ccagcgtttc gaatattatc ttatctttat aataatcatt ctcgtttacg
    10261 ttatcattca ctttacatca gagatatacc aatggcgcgt tccaaaactg ctcagccaaa
    10321 acactcactg cgtaaaatcg cagttgtagt agccacagcg gtaagcggca tgtctgttta
    10381 tgcacaggca gcggttgaac cgaaagaaga cactatcacc gttaccgctg cacctgcgcc
    10441 gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg cgacagtctg ctaccggcac
    10501 taaaaccgat acgccgattc aaaaagtgcc acagtctatt tctgttgtga ccgccgaaga
    10561 gatggcgctg catcagccga agtcggtaaa agaagcgctt agctacacgc cgggtgtctc
    10621 tgttggtacg cgtggcgcat ccaacaccta tgaccacctg atcattcgcg gctttgcggc
    10681 agaaggccaa agccagaata actatctgaa tggcctgaag ttgcagggca acttctataa
    10741 cgatgcggtc attgacccgt atatgctgga acgcgctgaa attatgcgtg gcccggtttc
    10801 cgtgctttac ggtaaaagca gtcctggcgg cctgttgaat atggtcagca agcgtccgac
    10861 caccgaaccg ctgaaagaag ttcagtttaa agccggtact gacagcctgt tccagactgg
    10921 ttttgacttt agcgattcgt tggatgatga cggtgtttac tcttatcgcc tgaccggtct
    10981 tgcgcgttct gccaatgccc agcagaaagg gtcagaagag cagcgttatg ctattgcacc
    11041 ggcgttcacc tggcgtccgg atgataaaac caattttacc ttcctttctt acttccagaa
    11101 cgagccggaa accggttatt acggctggtt gccgaaagag ggaaccgttg agccgctgcc
    11161 gaacggtaag cgtctgccga cagactttaa tgacggggcg aagaacaaca cctattctcg
    11221 taatgagaag atggtcggct acagcttcga tcacgaattt aacgacacct ttactgtgcg
    11281 tcagaacctg cgctttgctg aaaacaaaac ctcgcaaaac agcgtttatg gttacggcgt
    11341 ctgctccgat ccggcgaatg cttacagcaa acagtgtgcg gcattagcgc cagcggataa
    11401 aggccattat ctggcacgta aatacgtcgt tgatgatgag aagctgcaaa acttctccgt
    11461 tgatacccag ttgcagagca agtttgccac tggcgatatc gaccacaccc tgctgaccgg
    11521 tgtcgacttt atgcgtatgc gtaatgacat caacgcctgg tttggttacg acgactctgt
    11581 gccactgctc aatctgtaca atccggtgaa taccgatttc gacttcaatg ccaaagatcc
    11641 ggcaaactcc ggcccttacc gcattctgaa taaacagaaa caaacgggcg tttatgttca
    11701 ggatcaggcg cagtgggata aagtgctggt caccctgggc ggtcgttatg actgggcaga
    11761 tcaagaatct cttaaccgcg ttgctgggac gaccgataaa cgtgatgaca aacagtttac
    11821 ctggcgtggt ggtgttaact acctgtttga taatggtgta acaccttact tcagctatag
    11881 cgaatcgttt gaaccttctt cgcaagttgg gaaggatggt aatattttcg caccgtctaa
    11941 aggtaagcag tatgaagtcg gcgtgaaata tgtaccggaa gatcgtccga ttgtagttac
    12001 tggtgccgtg tataatctca ctaaaaccaa caacctgatg gcggaccctg agggttcctt
    12061 cttctcggtt gaaggtggcg agatccgcgc acgtggcgta gaaatcgaag cgaaagcggc
    12121 gctgtcggcg agtgttaacg tagtcggttc ttatacttac accgatgcgg aatacaccac
    12181 cgatactacc tataaaggca atacgcctgc acaggtgcca aaacacatgg cttcgctgtg
    12241 ggctgactat accttctttg acggtccgct ttcaggtctg acgctgggca ccggtggtcg
    12301 ttatactggc tccagctatg gtgatccggc taactccttt aaagtgggaa gttatacggt
    12361 cgtggatgcg ttagtgcgtt atgatctggc gcgagtcggc atggcgggct ccaacgtggc
    12421 gctgcatgtt aacaacctgt tcgatcgtga atacgtcgcc agctgcttta acacttatgg
    12481 ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct
    12541 cttttggggc acggatttcc gtgcccattt cacaagttgg ctgttatgca ggaatacacg
    12601 aatcattccg ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg
    12661 cttttgcatc cgctgtcgtt aacctttcct gccgggaaag tgaccggtct gattggtcac
    12721 aacggttctg gtaaatccac tctgctcaaa atgcttggcc gtcatcagcc tccgtcagaa
    12781 ggggagattc ttcttgatgc ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc
    12841 aaagtggctt atttgccgca gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg
    12901 gtggcgattg gtcgttaccc gtggcatggc gcgctggggc gctttggtgc ggcagatcgc
    12961 gaaaaggtcg aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc
    13021 gatagtctct ctggcggcga acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat
    13081 agccgttgtc tgttgctcga cgaaccgact tcggcgctgg atatcgccca ccaggttgat
    13141 gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc tgacggtcat tgccgtgctg
    13201 cacgatatca atatggcggc acgctactgt gattatctgg tcgccctgcg cggtggtgaa
    13261 atgattgctc agggaacgcc tgcggaaatt atgcgcggcg aaaccctcga aatgatttat
    13321 ggcatcccga tgggtatttt gccgcatccg gcgggtgctg cacctgtgag ttttgtttat
    13381 tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc
    13441 cgttgttatg gcagatgaat accgcccacg cggcggctat tgaccccaat cgtattgtgg
    13501 cgctggagtg gttgccggtg gaattactgc tggcgctcgg catcgtgcct tacggcgtgg
    13561 cggataccat caactatcgc ctgtgggtca gcgaaccgct attgccggac tcagtgattg
    13621 acgttggttt gcgtacagaa cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta
    13681 tggtctggtc ggcaggatat ggcccttcac cagaaatgct ggcacgtatt gcaccgggtc
    13741 gcggatttaa cttcagcgac ggcaaacagc cgctggcgat ggcgcgtaaa tcgctgacgg
    13801 aaatggcaga tttacttaac ctgcaaagcg cagcagaaac gcatttagcg caatatgaag
    13861 actttatccg cagcatgaaa ccccgctttg tgaagcgtgg tgcacgcccg ttattgctga
    13921 cgacgcttat cgatccgcgc catatgctgg ttttcggtcc aaacagcttg tttcaggaaa
    13981 ttctcgatga gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta
    14041 ccgccgtcag tatcgatcgt ctggcggcgt ataaagacgt tgatgtgctc tgttttgatc
    14101 acgacaacag caaagacatg gatgcgctaa tggcaacgcc gctgtggcag gccatgccgt
    14161 ttgtccgcgc cggacgcttt cagcgcgtac ctgcagtctg gttttatggt gcgacgctct
    14221 cggcaatgca ctttgtgcgc gttctggata acgccatcgg aggtaaagcg tgagtaaacg
    14281 aattgcgctt tttccggcgt tattgctggc gctgttggtg attgccgcta cggcgctcac
    14341 ctggatgaat ttttcgcagg cgctgccgcg cagccagtgg gcgcaggcca cctggtcgcc
    14401 ggatattgac gtcatcgagc agatgatttt tcactacagc ttgttgccgc gtctggcgat
    14461 ttcgctgctg gtgggcgcgg gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg
    14521 taacccgctg gcggagccga cgacgcttgg cgttgctaca ggcgcgcaac tgggaattac
    14581 cgtcaccaca ctttgggcga tcccaggtgc tatggcgagc cagtttgccg cgctggtggg
    14641 cgcttgtgtc gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt
    14701 tacgcttatt ctcgcggggc tggtggtgag cctttattgc ggggcaatta atcagctact
    14761 ggttatcttc catcacgacc agctgcaaag catgttcctg tggagcaccg gaacgctgac
    14821 gcaaaccgac tggggcggcg ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt
    14881 gacgttattg ctacttcgcc cgttaaccct gatggggatt gatgatggcg tggcgcgcaa
    14941 tctcgggctg gccttgtcgc tcgcgcgtct ggcagcgctg tcgctggcga ttgtcatcag
    15001 tgcgctgctg gtgaacgctg tggggattat cggctttatc gggttgttcg cgccgctgct
    15061 ggcaaaaatg ctgggggcgc ggcgtctgct gccacgactg atgctggcgt cgctgattgg
    15121 ggcgctgatc ctctggcttt ccgatcaaat catcctctgg ctgactcgcg tgtggatgga
    15181 agtgtccacc ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc
    15241 gcgtttacgc agcattagcg cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg
    15301 ccaacatgtg ctggcgtttg ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt
    15361 ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg gcgagcgggg cgttgcttga
    15421 ggatttgatg ccctggcgct ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct
    15481 ggcggtggcg ggctgtatta ttcagcggct gaccggaaac ccgatggcaa gcccggaagt
    15541 gctggggatt agctctggcg cagcgtttgg cgtggtgttg atgctgtttc tggtgccggg
    15601 taatgccttt ggctggctgt tacctgcagg cagtctcggc gcggcggtga cgctgttgat
    15661 cattatgatc gccgccggcc gcggtggatt ttccccacac cgcatgttac tggcggggat
    15721 ggcgttaagc accgcgttca ccatgctttt gatgatgttg caggcaagtg gtgacccgcg
    15781 aatggcgcaa gtgctgacct ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt
    15841 ctggcgcacg ggaattgtga tggtgatttt gctggcgatt accccgctgt gccgccgctg
    15901 gctgaccatt ttaccgctgg gtggtgatac cgcccgagcc gtaggaatgg cgctgacgcc
    15961 gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg gcgaccgcga cgatgactat
    16021 tggaccgttg agttttgttg gtttaatggc accgcatatt gcgcggatga tgggctttcg
    16081 acggacgatg ccacacatcg taatttcggc gctggtgggt ggtttactgc tggtgttcgc
    16141 tgactggtgt gggcggatgg tgctgtttcc attccagatc ccggcagggc tgctgtcgac
    16201 ctttatcggc gcgccatatt ttatctattt gttgagaaag cagatccgtt aatagaatgc
    16261 ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa acacccgtcg tgcagcatcg
    16321 atggtgttat tgatatcttc catgctgtgc gccacggaca taaagcccgc ttcaaacgct
    16381 gacggtgcca ggtaaacacc ttcgtccagc atcatatgga agaaacgctt aaagcgttcc
    16441 acgtcacagg ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata
    16501 ccgaacatgc cgccaacgtg gttaacgacc agcggagttc cggcttcttc tgccgcttcc
    16561 agcagacctt ctgccagacg tgatgtcagc tcatccaacg tttcgtgaac gcccggctgc
    16621 gcgacttcat tcagacaggc gaaacccgct gccatcgcaa ttgggttacc ggaaagcgta
    16681 cccgcctgat agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg
    16741 aatgcgccta ccggcattcc accgccgatg attttgccga ggcaggttaa atctggcact
    16801 acgccgtaat aatcctgtgc gccagctagc gctacgcgga aaccggtcat cacttcatcg
    16861 atgatcagca acgcgccaaa ttcgtcgcac agcgcgcgca gacctggcag gaactctggc
    16921 agcggcggaa cacagttcat attgcctgcc accggctcga cgataataca ggcaatctct
    16981 tgcgggtatt gctcaaatgc ggcgcgtaca gaagccagat cattataagt acaggttaag
    17041 gtatgtttgg cgaaatctgc cggaacgccc ggcgagtttg gctggcctaa cgtgagtgcg
    17101 ccagaaccgg ctttcaccag caggcagtca gcgtgaccat ggtaacaccc ttcaaattta
    17161 ataattttgt cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat ggtcgcttca
    17221 gtgccggagt tcaccatgcg caccatatcc atggtcggga ccagttcggt caccagttgc
    17281 gccattttca cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca
    17341 atcacggcat tgcggattgc cggatggtta tggcccagca ccatcggccc ccaggaaccg
    17401 acataatcga tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg
    17461 ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca
    17521 gggatcagct cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga
    17581 ttcgtagaaa aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc
    17641 aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc
    17701 atttgaaaca ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat
    17761 cttatttgta tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac
    17821 accgcaggcc gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa
    17881 aaccccgtta gccattttgt ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc
    17941 ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca
    18001 tactgctgat aattatccgc ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc
    18061 gatgttcggc tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc
    18121 ggaaattgaa ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt
    18181 gaaattcttt ggcgggctgg ggacactcgg cggaggcatg gtgctagggc gcgaaggacc
    18241 aaccgtgcag atcggcggca atatcggtcg catggtgctt gatattttcc gcctgaaagg
    18301 tgatgaagct cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt
    18361 taacgcgccg ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta
    18421 tacgttaatt tcgattaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg
    18481 gatttttaat catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa
    18541 tacgctgtgg ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa
    18601 taaatgggtg ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa
    18661 atgggtactg atgggtggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc
    18721 agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat
    18781 gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc accttactct gcttctcttc
    18841 cggcgcgccg ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc
    18901 tttcggaatg gttgccgttg agctgtttcc gcaatatcac ctggaggcgg ggacgtttgc
    18961 tattgccgga atgggggcat tactggcggc atctattcgc gcgccgttaa cggggatcat
    19021 tctggttctg gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct
    19081 tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg ctatactcgg cgattcttgc
    19141 gcgcacgctg gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag
    19201 cgagaatact tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa
    19261 tttgcccaat tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct
    19321 ggagtttacc gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc
    19381 gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt
    19441 cacctttgat gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggcct
    19501 ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt tccgttgact acaccgaagg
    19561 actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg
    19621 ttcttccttt agcatctaat ctgttgttca tgtgccggat aaactatccg gcacatcttc
    19681 tctcttcaac gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata
    19741 gccgccagcc gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt
    19801 acggaaaatg tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg
    19861 tagatttctg tacgtaaaat catggggatc tcgcgggcca gtacatcacg aatgatcccg
    19921 ccgccaacgc cagtaatgac gcccatgcaa accgcgatta acggaccggc ttccgcatta
    19981 aaggctttat tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc
    20041 aacatccatt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtg
    20101 accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc
    20161 gccatgtcgc gaattgtccc gccgcctact gcggttacca cgcccagtac cagaacacca
    20221 aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg
    20281 ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac ctgaaaaggt
    20341 caggcgctaa tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg
    20401 tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    20461 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    20521 tggcgagcgt gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt
    20581 aaagatgttt tctccgccac aaacttcgag tacctggttc tgaatcgact cttttccact
    20641 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    20701 ttgcgctttc aattgcgcgt actgatcctg cagggattgc gcggcttgtt cggccttgtc
    20761 tggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt
    20821 cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc
    20881 attacctcca cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt
    20941 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    21001 gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga
    21061 aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag acagggcgac
    21121 cagcgccctg aacagtgact tagccatgtg caagtttctg caccagtgac tcaaccatca
    21181 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    21241 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    21301 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    21361 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    21421 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    21481 taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    21541 cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc cactttcagc gttggtgcca
    21601 ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcaaag
    21661 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    21721 cattcagttg gccggtatag atttcgcaac cgcccagact gatagtttga cggttttcga
    21781 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    21841 atttactcgc gataagcccg atttgaaggc atagtttacc atgcgcttac ggggaagcgt
    21901 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    21961 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    22021 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacgcag
    22081 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    22141 cagcaggtgg ggcgctacat cgccaaagaa attttaagcc gtctgaaaga gcttaaattg
    22201 ctggaagcat acggcctgga tgaactgacc ggaccttttg aaagcattgt tgagatgtca
    22261 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc agcgataaat
    22321 gactggtttc gccaacgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat
    22381 cgctgcagcg tggcggcact acgtttacgg gacggggaag aaccgcttaa cgagctgcgg
    22441 cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg
    22501 catacattga tgcggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc
    22561 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    22621 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    22681 ctttacagtc gttttccatt aacgtggatt atggaagctg ccgacgatat ctcctattgt
    22741 gtggcagacc ttgaagatgc agtagagaaa agaatattta ccgttgagca gctttatcat
    22801 catttgcacg aagcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa
    22861 aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt
    22921 atgtatttac gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt
    22981 gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc
    23041 gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat
    23101 ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt
    23161 tatcgtcctt tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg
    23221 gtgaaacgtt tccctattga atcgcgctta ttccacaaac tctcgacgcg ccatcggctg
    23281 gcctatgtcg aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa
    23341 tattattacc gttgccgcct gttgcaggat tatatcagcg gtatgaccga cctctatgcg
    23401 tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa
    23461 caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc
    23521 tgaagaacac agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc
    23581 acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca
    23641 acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg
    23701 ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat
    23761 acaccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag
    23821 gaaggttctc cgttccagag ctctccgttc tgccaggggg gccagggcgg taatggcggc
    23881 ggccagcaac aaaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc
    23941 tatgtcgtca ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc
    24001 gatggtcgta agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg
    24061 atccaaatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg
    24121 cgcgtgggtg attacaccgt agcgattggt aacccgtttg gtctgggaga gacggtaact
    24181 tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc
    24241 atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac
    24301 ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc
    24361 ggttttgcta tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc
    24421 caggtgaaac gcggtgagct gggtattatg gggactgagc tgaattccga actggcgaaa
    24481 gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc
    24541 gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccaatc
    24601 agcagctttg ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc
    24661 ctgggcttgc tgcgcgacgg taagcaggtc aacgtgaatc tggaactgca gcagagcagc
    24721 cagaatcagg ttgattccag ctccatcttc aacggcattg aaggtgctga gatgagcaac
    24781 aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag
    24841 atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc
    24901 gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc
    24961 ggcgacagct ccatctacct gttaatgcag taatctccct caaccccttc ctgaaaacgg
    25021 gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct
    25081 ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg
    25141 aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    25201 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag
    25261 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    25321 acgagtcgtc gatatcgatg acgcggtggc acgccatctg cacggtgtgc ggcaggggat
    25381 taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    25441 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    25501 gtcgcggttg atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa
    25561 cctgattcag gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    25621 cgatctcaat caaccgcgag tagtggctat tgttgaggtc gacagcggtc agcttggcgt
    25681 ggacagcgca atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa
    25741 taatctggtg gcgattgtct cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc
    25801 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    25861 gaaagagtat ggccagctgc gttttcgcgt ttcgctgggc aactatttta ccggtcctgg
    25921 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    25981 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    26041 gcgtggcgac tggcaggcca atgaactggc gcgaccgctg gcgcggctga aagcgatgga
    26101 caataatggc ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct
    26161 ggctacgtca aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat
    26221 atcggaactg accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc
    26281 gttgcagctg gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt
    26341 cccctcagcc cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta
    26401 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    26461 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    26521 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    26581 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    26641 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26701 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26761 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26821 agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26881 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggcgata ttccatggga
    26941 tggattaaca catttatctt cgttttgcga attacatgac agtccgaaac aagatgaaaa
    27001 agtatttaaa aaagggggga agaagaatga gtaggacgaa ttattctgag ccagaaagca
    27061 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
    27121 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    27181 ggctgtattt gccatctttc gacggcagat tacctgaaac aaccacagac cccgccggaa
    27241 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    27301 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcggagc
    27361 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    27421 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    27481 cgacggtcgc ccaggtatca accatcgtgc cttcatcaac atatgcgccg atgttgacgt
    27541 aagacggcat cagcacggtg ttacgggcaa taaatgcacc ctgacgtacc gccgctggtg
    27601 gcacaacgcg gaagccttct ttctgaaaac gcgcttcgtc gtagtcggcg aatttcatcg
    27661 gcactttatc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    27721 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    27781 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    27841 ttacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    27901 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    27961 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    28021 gtgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    28081 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actctcgcca
    28141 gcagtcctgg ctggtcgagc gcgataagct caaggaacga tttgcggtcg gtatgggtcg
    28201 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    28261 gctgcggtgg ctgccagcta ctttgcgtca gtacttgctc cagaccaaac cgaataacct
    28321 catgacgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    28381 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    28441 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    28501 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
    28561 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    28621 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    28681 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    28741 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    28801 tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    28861 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    28921 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    28981 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    29041 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    29101 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    29161 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    29221 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    29281 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag cccgcgccgc accgctccgg
    29341 ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac agcggttgtt
    29401 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    29461 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    29521 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgattggac
    29581 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    29641 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    29701 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    29761 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    29821 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    29881 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    29941 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    30001 aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc gtagaacttg tcggaaggcc
    30061 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    30121 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccagcatgc
    30181 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    30241 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    30301 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcgaccaatg
    30361 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcga
    30421 taaactcggt gcgcgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    30481 ccagccaacg ctggaaagta tcgatatggg cttttatccc accgacggtt aattcatcac
    30541 ggggccagac gcatggattt tgcggttgac cgggcagggt ggggagagcg gtgtttgcgt
    30601 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    30661 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    30721 tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    30781 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    30841 tcttttttac ccgcgttgac cattggctcg atggtgaacg tcatcccagg tttcagtacg
    30901 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    30961 ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaatttctga
    31021 atcgccgcac cgatttcgcg cagattaatg cctggtttga ccatgcgcag cgccaggtac
    31081 aggctttctt gcgtgatgcg gcacagacgt tcgcccatga tggtcggctt accgacgata
    31141 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    31201 atatcgccat ctttcagcag cttcgcatcg tccgggatac cgtggcacac cacttcatta
    31261 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    31321 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    31381 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    31441 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    31501 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    31561 tcattctgaa tttcgccaaa cgtgccactg aacgttttct ataatagaaa attcaacgtc
    31621 tgatgctgta cacagcgcca acaattattg gtgtccatga cgtatttgtg gtataaagcg
    31681 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    31741 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    31801 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    31861 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    31921 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    31981 actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc tcgcaaaggt
    32041 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    32101 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    32161 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    32221 ttcgaaaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    32281 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    32341 gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc ggtatttgct
    32401 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    32461 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    32521 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    32581 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    32641 cttacatcag taagtgaccg ggatgagcga gcgaagacaa cgcatctgcg gcgcgaaata
    32701 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    32761 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    32821 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    32881 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    32941 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    33001 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    33061 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    33121 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    33181 aacgtgttgc gctggtagcg aaaattggtg agaacatcaa cattcgccgc gttgctgcgc
    33241 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    33301 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    33361 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    33421 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    33481 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    33541 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    33601 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    33661 cgatgtccaa gcagtcttaa ttatcgaaaa ggagccgcct gagggcggct tctttttgtg
    33721 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    33781 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    33841 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    33901 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    33961 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    34021 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    34081 aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    34141 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    34201 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    34261 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    34321 caaagttgac ggcgtgttta ctgctgatcc ggcgaaagat ccaactgcaa ccatgtacga
    34381 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    34441 cacgctggct cgtgaccata aattaccgat tcgtgttttc aacatgaaca aaccgggtgc
    34501 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    34561 atggataaat aagggtaaga ttccgcataa gtatcgcggg ggcgtaagtc tggttataag
    34621 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggtg cgtacacaag
    34681 tacgtgagga tggcgagcac tacccggggc caaaatggca aataaaatag cctaataatc
    34741 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    34801 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    34861 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    34921 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    34981 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    35041 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    35101 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    35161 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    35221 tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    35281 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    35341 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    35401 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    35461 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    35521 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    35581 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    35641 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    35701 agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    35761 cgcttgagga tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta
    35821 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    35881 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    35941 atagcgaaca taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    36001 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    36061 tccgtgagac gccattgcgc gatttggcaa caatgacgcc ggatcaagcc tgccgtcatc
    36121 cgaactggtc gatggggcgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    36181 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    36241 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    36301 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    36361 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    36421 cggattatga tcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggtcagg
    36481 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    36541 aaatccgctt tacggatatc gctgcgttga atttatccgt actggaaaaa atggatatgc
    36601 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca
    36661 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    36721 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    36781 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    36841 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    36901 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    36961 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    37021 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    37081 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    37141 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    37201 tattggcgat accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    37261 gctaacagcc gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    37321 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    37381 agatcagata gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt
    37441 agatttagta attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat
    37501 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    37561 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    37621 tgatgaaaca gcctgatggg gggcgctttt gctgaagtat cgcctgatat ctgcttttgt
    37681 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccatcgt
    37741 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    37801 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    37861 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    37921 ttcgctgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    37981 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    38041 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    38101 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    38161 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    38221 aggctttata ggtggactag cgaccgcagc ggtaatctca tggggttatg gcatgtgggc
    38281 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    38341 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    38401 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    38461 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    38521 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    38581 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    38641 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    38701 gccctgatcc cgctgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    38761 ccggaactcc gccaccatgc cttcaataat aaatctgttg gccaacgagc ggcgattatt
    38821 gccgcaggtc cggttgcaaa cttcattttt gctatctttg cctactggct ggtttttatt
    38881 attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg
    38941 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    39001 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    39061 gtagcgccat ttggcagcga tcaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    39121 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    39181 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    39241 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    39301 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcagggaagt
    39361 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    39421 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    39481 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    39541 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    39601 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    39661 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    39721 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    39781 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    39841 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    39901 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    39961 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    40021 cggccctcct cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta
    40081 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    40141 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    40201 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    40261 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    40321 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    40381 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    40441 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    40501 acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    40561 gcgaccttga aaccctgcgc agctactatc tggatcgcgg ttatgcccgt ttcaacatcg
    40621 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatta
    40681 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    40741 ccgctgaaat tgagcagctg actaagatcg agccgggcga gctgtataac ggcaccaaag
    40801 tgaccaagat ggaagatgac atcaaaaagc ttctcggtcg ctatggttat gcctatccgc
    40861 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    40921 ttgatgcggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    40981 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg ggaagcgatc
    41041 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    41101 atacccaacg tgttccgggt agcccggacc aggtagatgt cgtctacaag gtaaaagagc
    41161 gcaacaccgg tagcttcaac tttggtattg gttacggtac tgaaagtggc gtgagcttcc
    41221 aggctggtgt gcagcaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    41281 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcactgtag
    41341 atggtgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    41401 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg
    41461 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    41521 ctcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    41581 aggataatag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    41641 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    41701 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    41761 tcgatgacga tcacaaatgg gttgttctgg ggcgtacccg ctggggttat ggtgatggtt
    41821 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    41881 gtggcttcca gtccaataca attggtccga aagcagttta cttcccgcat caggccagta
    41941 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    42001 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    42061 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata
    42121 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    42181 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    42241 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    42301 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    42361 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    42421 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    42481 ctcggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaacatg
    42541 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag
    42601 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    42661 aagctgcagt ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct
    42721 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgtgc acgtcgttcc
    42781 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    42841 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    42901 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    42961 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    43021 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    43081 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    43141 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    43201 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc
    43261 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    43321 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    43381 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    43441 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    43501 aacgatcgtg gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc
    43561 gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tgttattggc
    43621 aatggcgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    43681 acggcggttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    43741 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    43801 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    43861 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    43921 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    43981 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    44041 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    44101 ttgaagagat tttagaactt ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc
    44161 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    44221 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    44281 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    44341 tgtactactt cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc
    44401 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    44461 ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    44521 aggcctgata agtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    44581 gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg
    44641 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    44701 gccgcgataa tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat
    44761 atgctggcga accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    44821 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    44881 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca
    44941 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    45001 ccgcagtcca tcagttctgc atcattggtg cgcacgtgat ggttggcggc tgctccggtg
    45061 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    45121 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    45181 atgcgtataa gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    45241 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    45301 cgcgcggtct gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg
    45361 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    45421 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    45481 gtacgaaatg gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    45541 cttactgcat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatatttt
    45601 tgttggtatt gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    45661 tatcaaaacc attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt
    45721 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    45781 tgacaaatac aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt
    45841 agatccagat aaaaatgccg cccgtgatgt gctggggatc cctcacgatg cccactgcct
    45901 ggcgttgcta ccagggagcc gtggtgcaga agttgaaatg cttagtgccg atttcctgaa
    45961 aacggcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cactggtgaa
    46021 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctttcagt
    46081 tcatttgctg gatgggatgg gccgtgaggc gatggtcgcc agcgatgcgg cgctactggc
    46141 gtcgggtacg gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg
    46201 catgaagcct tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct
    46261 gccaaatctg ctggcgggta gagagttagt caaagaatta ttgcaggaag agtgtgagcc
    46321 gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat
    46381 gcacgatacc ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    46441 acaagccgtt ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt
    46501 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    46561 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    46621 cgccgtctgg cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    46681 gcggaacccc acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag
    46741 cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    46801 ccgaaattac cgatgcctgc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc
    46861 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    46921 gttttcccgc aatatggttt tgcccaacac aaagggtacc caaccgcttt tcatctggaa
    46981 aaactggctg aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc
    47041 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    47101 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg
    47161 gccaaaaccg caccgttggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc
    47221 accgatttca ccaacctttg tggtctggtg aagttctacg gagcgggaca tggcgcaggg
    47281 attaagccta tcgtcggggc agattttaac gtccagtgcg acctgctggg tgatgagtta
    47341 acccacctga cggtactggc ggcgaacaat accggctatc agaatctgac gttgctgatc
    47401 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    47461 atcgaattaa acgaagggtt gatccttctt tccggcggac gcatgggcga cgtcggacgc
    47521 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    47581 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggatga agaaagctat
    47641 ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gaccaacgac
    47701 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    47761 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    47821 agcgaagagg agatgtgtga gctgtttgcc gacatccccg aagcccttgc caacaccgtt
    47881 gagatcgcca aacgctgtaa cgtaaccgtg cgtcttggtg aatacttcct gccgcagttc
    47941 ccgaccgggg acatgagcac cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa
    48001 gagcgtctgg cctttttatt ccctgacgag gaagaacgtg ttaagcgccg cccggaatat
    48061 gacgaacgtc tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc
    48121 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc
    48181 cgtggctccg gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg
    48241 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    48301 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta ttgagcacgt agcggacatg
    48361 tacggtcgtg atgcggtatc gcagatcatc accttcggta caatggcggc gaaagcggtg
    48421 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa
    48481 ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    48541 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    48601 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    48661 attaccgatt ttgcgccgct ttactgcgat gaagagggca aacatccggt cacccagttt
    48721 gataaaagcg acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg
    48781 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    48841 ccgccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    48901 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    48961 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcgctgtt ccgcccaggt
    49021 ccgttgcaat cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagaaatc
    49081 tcctatccgg acgtacagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac
    49141 ggcatcatcc tgtatcagga acaggtcatg cagattgcgc aggtgctttc tggttatacc
    49201 ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct
    49261 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    49321 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct
    49381 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tcctgcggag
    49441 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    49501 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    49561 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgccat caaaggggta
    49621 ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    49681 ctgtttgatc tttgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    49741 ctgatcatgt ccggggcgtt tgaccgtctt ggaccacacc gcgcggcact gatgaactcg
    49801 ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    49861 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    49921 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg cctgtacctg
    49981 accggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    50041 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    50101 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    50161 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    50221 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    50281 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    50341 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    50401 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    50461 gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    50521 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    50581 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    50641 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    50701 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacacgtaa aatcttcgcc
    50761 gatctcggtg catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg
    50821 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca
    50881 gacgataaag ctatcgtcgg tggtatcgcc cgtcttgacg gtcgtccggt gatgatcatt
    50941 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    51001 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcct
    51061 atcatcacct ttatcgacac cccgggggct tatcctggcg tgggcgcaga agagcgtggt
    51121 cagtctgaag ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt
    51181 tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa
    51241 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    51301 ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    51361 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg cggcgctcac
    51421 cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat
    51481 ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    51541 ggttacgcgt aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg
    51601 ccacggtttg agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca
    51661 ttgccattat gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt
    51721 cggcgctggt ggcgcaaggc tttcagatta tctggccaca aaacagcgtt gatttgctga
    51781 aatttatcga acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc
    51841 tcgatttatg cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca
    51901 acacccactc gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg
    51961 aatatgcgct ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accgacgaat
    52021 atcttgataa cattacaccg ccgttcacga aagccttgtt tacctacgtt aaagagcgga
    52081 agtacacctt ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg
    52141 gctgcctgtt ttatgatttt ttcggcggga ataccctcaa ggctgatgtc tctatttcgg
    52201 tcaccgagct tggttcgttg ctcgaccaca ccgggccaca cctggaagcg gaagagtaca
    52261 tcgcgcggac ttttggcgcg gaacagagtt atatcgttac caacggaaca tcgacgtcga
    52321 acaaaattgt gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaatt
    52381 gtcataaatc gctggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac
    52441 cgacgcgtaa tgcgttgggg attcttggtg ggatcccgcg ccgtgaattt actcgcgaca
    52501 gcatcgaaga gaaagtcgct gccaccacgc aggcacaatg gccggttcat gcggtgatta
    52561 ccaactccac ctatgatggc ttgctctaca acaccgactg gatcaaacag acgctggatg
    52621 tcccgtcgat ccacttcgat tccgcctggg tgccgtacac ccattttcat ccgatctacc
    52681 aggggaaaag tggtatgagc ggcgagcgtg ttgcgggaaa agtgatcttc gaaacgcaat
    52741 cgacccacaa aatgctggcg gcgttatcgc aggcttcgct gatccacatt aaaggcgagt
    52801 atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc
    52861 ccattgttgc ttcggttgag acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc
    52921 tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggttgcggg
    52981 aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat
    53041 gctggcccgt tgcgccaggc gaacagtggc acggctttaa cgatgcggat gccgatcata
    53101 tgtttctcga tccggttaaa gtcactattt tgacaccggg gatggacgaa cagggcaata
    53161 tgagcgagga ggggatcccg gcagcgctgg tggcaaaatt cctcgacgaa cgtgggatcg
    53221 tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggt atcgataaaa
    53281 ccaaagcaat gggattattg cgtgggttga cggaatttaa acgctcttac gatctcaacc
    53341 tgcggatcaa aaatatgcta cccgatctct atgctgaaga tcccgatttc taccgcaata
    53401 tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac gatcttcccg
    53461 gtttgatgtt gcgggcattc gatactttgc cggaaatgat catgacgcca catcaggcat
    53521 ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag
    53581 tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa
    53641 tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt tccgtagggc
    53701 aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt
    53761 accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttcc gggcgagtaa
    53821 cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc
    53881 gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac
    53941 gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa
    54001 tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc
    54061 ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc
    54121 gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa
    54181 acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg
    54241 cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca
    54301 tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattctg gtggccttta
    54361 gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc
    54421 cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct
    54481 gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg
    54541 tacaacttgc gcaagagggg ctggggattg aggcccaagc gcggcaggcg cgttatcagg
    54601 catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc
    54661 aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta
    54721 tgggggaagt ctcggagttt gccggaacac ggcttatccg cccgttgctt gcccgcacgc
    54781 ggggggaact ggcgcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta
    54841 atcaggacga ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc
    54901 agcgttggcc gcattttgcc gaagcaacgg cccgcagcgc cgcactttgt gctgaacaag
    54961 agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga
    55021 cgctgcagat tgcgccaatg ctagcgatga gtgatgcccg tcgcgcggcg attatccgcc
    55081 gctggctggc agggcagaat gcaccgatgc cttcccgcga cgcgctggtg cggatctggc
    55141 aggaagtggc gctggcgcgg gaagatgcct ccccctgttt acgttttggc gcgtttgaaa
    55201 tacggcgcta tcagtcgcaa ctgtggtgga ttaactccgt cactgggcaa agcgaaacca
    55261 ttgtgccgtg gcagacgtgg ctacaaccgc tggaattacc ggcggggctg ggaagtgtac
    55321 agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt
    55381 tcaaagcgcc aggattgctg catattgtcg ggcgtaacgg cggacgtaag ctaaagaaaa
    55441 tctggcaaga gctgggcgtg ccgccgtggc tacgtgacac cacgccactg ctgttttatg
    55501 gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg
    55561 agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg
    55621 catcaaacgt cgcatccggc gaaagtcaat caggactcgc tcaccaccac cgtaccgatt
    55681 tccgggtggc taaagctggt aattttatcc agacgtagct cacgggtttc gccagcggcc
    55741 tcgacgacca gatactccac atttttgcgg gagactaaat cgctggcttt tgcctgcaat
    55801 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    55861 ttatcgtaat catcacaatt gattggttga tacgtatcat tcattgacat aatcgctcac
    55921 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    55981 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    56041 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    56101 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    56161 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    56221 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    56281 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    56341 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cttgctggcg
    56401 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    56461 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    56521 ccggatgagc ggcggattaa gaaagcctgc acccaggccg cagaagtggt gctgtttacc
    56581 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt
    56641 gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc
    56701 gatcgtacca tgacgctgca ggcaacgatt caggatggcg tgatctggtt atcggacgat
    56761 aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    56821 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgcgg
    56881 gcgggcagca tgttaataag acctcaacgg ccattcatct gcgttttgac attcgggcgt
    56941 ccagcctgcc agagtattac aaagagcgtc tactcgccgc cagccatcat ttgatcagca
    57001 gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    57061 ccgcgctagc ccggctggtg gcagtgatta aagatttaac aacagaacaa aaagcccgac
    57121 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    57181 caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatag
    57241 atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga
    57301 tgtaataatc gggccgaagt tgatgcgctt tctccggcgc aggctgccga actgaaaccg
    57361 atgccgcaaa gttggcgcgg cgtgctgccg tgtgctgatt gcgaaggaat cgaaacctct
    57421 ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa
    57481 gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta
    57541 accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc
    57601 gatcgtgaag gcaatccgat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc
    57661 agtttaccta tgacgccgat gaccctgcgg ggcatgtatt tttatatggc tgatgcggcg
    57721 accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag
    57781 cgtggctacc tggctgcgcg cggccacagt gaaaaaccga tgttactgtc agtagaaggt
    57841 cactttacgc ttgaagctaa tccggatacc ggtgcgccga ctaaagtatt ggcacccgat
    57901 acggcaggga aattttaccc aaaccaggat tgcagtagtt tggggcagta aacctgctta
    57961 aggcagaaac aaacgcaaaa ttgcctgatg tgctgcgctt atcaggccta cagcatataa
    58021 tgcaagattt tgaattagca cgattttgta ggccggataa ggcgtttacg ccgcatccgg
    58081 cattttgtgc acgaggctgg ctttatcact gagtcagccc aacaaagcga cccgccttga
    58141 tataaatccc aggcttcagg tgcccaacat actgtaactg cgtttctggc ttaaacgcag
    58201 agacatcacc aatacgcata tgcatcagat cggcagggct aatccagccc gattgcgcca
    58261 gtgtaagtgg atgcccggct ttggcaaagg catccgtgac aaattccgaa caaaaccacg
    58321 actttttgtc tccttcgccc acactgctta actgcgcttt cgccaggccg ctgacgcact
    58381 ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca ccataaaggg
    58441 aataaattcg acaatgccgc gatagttata accgctatct ttgattttat tggcaaacgc
    58501 ggtgatatct gtggcttgtt gcggggtaag atccgggact cgtaagacga aaagcttatc
    58561 actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg tcgcttctgc
    58621 aacgttatta tcacccagaa agattgcaac gtgactcaca gaggaagtgc tgaagacgcg
    58681 gattccaaat gaggttaccc caaggcttga ggagaacagc aaatcgccgg gtttgagatc
    58741 aggtgccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct ggaatttgac
    58801 agcccatgtt tttgcctcgg catctactgc tgtggctgat gggtctggct ggctgatatc
    58861 aacggtacaa gccgaaagta aaagaaagct ggggagaaac aggcggcagt acgcctttgg
    58921 tttatccatt ttatacaatc catgtaaaaa aagggccctg aaattcagga ccctttctgg
    58981 catcagcctt taatctgttt caccagatat tcgacgatgt caccagtctt aattaactgt
    59041 ttctcgccgt tgcgacgata tttatattcg atatcgtcgt tgtcgaggtt acggtcgccc
    59101 agcacaatag tgtgcggaat accgatcagt tccatatcag caaacatcac gcccggacgc
    59161 tctttgcggt catccagcag cacttcgata ccttgtgcac gcagttcgct gtacagtttc
    59221 tcagcaagct cttgtacgcg gaaggatttg tgcatgttca tcggcagaat cgccacctgg
    59281 aacggcgcga tagcgtcagg ccatacgatg cctcgttcgt cgtagttctg ctcaatcgcc
    59341 gcagctacca cacgcgttac cccgataccg tagcaaccca tcgtcaggat ttggttacgg
    59401 ccatcttcac cctgtacgga ggctttcagt gcttcggagt acttggtacc cagctggaag
    59461 atgtgaccaa cttcgatacc acgtttgatc agcagcgtac cctggccatc cgggcttgga
    59521 tcgccagcca ccacgttacg gatatctgca acttccgggg tagcgacatc gcgatcccag
    59581 ttgatgccga agtagtgttt accatcgatg ttagcaccag cagcgaaatc actcatcgcc
    59641 gcaacggtac ggtcaatcac caccggaatc ggcatgttta ccggacccag tgaacccgga
    59701 ccggctttaa ccacggcacg aatttcttct tcggtcgcga aagtcagcgg gcttgcaacc
    59761 tgcggcagtt tttctgcttt aacttcgttc agctcgtgat caccgcgcac cagcagcgca
    59821 accagcggga agctactgcc ttcaaccgct ttaaccagca gagtcttaac cgttttctca
    59881 atcggcagat tgaactgttc aaccagttcc gcgatggttt tcgcgttcgg cgtatcaacc
    59941 agcgtcattt cctgggtagc agcagcgcgc ggttctttcg gcgcgatagc ttctgccagt
    60001 tcaatgttcg ctgcatagtc agaggtgtcg gagaagacca catcgtcttc accgctctgc
    60061 gccagcacct ggaattcgtg agaggcgctg ccgccgatag aaccggtgtc ggcttgtacg
    60121 gcgcggaaat ccagccccat gcggctgaag attttgctgt aggccgcata cattgcatcg
    60181 taggtttcct gcagggattc ctgagaagta tggaaagagt aagcatcttt catcaggaat
    60241 tcgcgggaac gcatgacgcc gaaacgcgga cgcacttcgt cgcggaactt ggtctggatc
    60301 tgatagaagt tcagcggcag ctgtttgtaa gagctaagct cgttacgaat caggtcagtg
    60361 ataacttctt catgagttgg gccgagtacg aacggacgct cgccacggtc aacaaaacgc
    60421 agcagttccg gaccgtactg ttcccaacga ccactctctt gccacaaatc agctggctga
    60481 accaccggca tcgacacctc gatcgcaccg gcgttgttca tctcttcacg cacgatgttt
    60541 tcgacttttt tcagaacgcg cacgccggtc ggcagccagg tatataaccc ggaggccagc
    60601 ttgcggatca tcccggcgcg cagcatcagc tgatggctga tcacctcggc gtcggcaggt
    60661 gtctccttga gagtggagag caggtattgg ctagtacgca tgttgttacg gttccagttg
    60721 gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa agtgatttag tttaccagtg
    60781 caaaagaaat gtcaaaagag aagggcgtga atttaacgcg gttccagcgc aaagacttca
    60841 aaacctacgt cggtgacgcg ccagcgaacg ttaaaatcat gtagccagac ggcataggtt
    60901 ttgcccgttt cctcaccttt acgataggcc gggcgtgggt cctgcgccag tacttcgcgg
    60961 ataaacagcg ttaactgcgg ataacgcttc tccagcgtca aaagctgctt ttcgacctct
    61021 gcggtaaaac tcaccgccat ctctgccgct ggcgcacttt gcgcatagct ggcactggca
    61081 tcgggaagcg attcggcaaa ggggagatac ggtttgatat ccactaccgg cgtaccatcg
    61141 accagatcca gactgccgag cttcagaatc acgctgtctt tatggcaaac aacctctttc
    61201 agctctacca gcgacatacc aattgggtta gggcggaaag tagagcgtgt tgcgaaaacc
    61261 cccattctgg cgttaccgcc gaggcgcggt ggacgcacag tcggacgcca gccgccttcc
    61321 atcgtttgat gaaagacgaa aaggatccat aaatggctga acgcttccag gccgcgaacg
    61381 gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc cgttggcgct ttttaccaga
    61441 cctggctggc gcggaacggc gaatttttct ttatagggcg agcgaataac gcctatttgc
    61501 tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc agaaccgata catacagcct
    61561 gacgatagca gcctggcgta ccgctggtga cttcgcagct atgcagtaat accgcattgg
    61621 ccttcatttt cgaggcgttg atttgcatcc gcttacgtgc ggttggaatg ctcggcggag
    61681 agtcctgatt ggaggcctgg caagaatcgc cactgacttc accgagatcg cggaacggtt
    61741 tgccgactaa ttcttctgca ttggtataaa ttcggaccgg cgtggcgcgc ggcgctttcg
    61801 gttttgcagg ctccgctttc ggctggggtg cagtgctttg aacgggttcg acaggggatc
    61861 tgcttaacat ggaacagccg cttagcatga gtgctactaa acagatcggt aaagcacgca
    61921 tagtatttcc tcaatgtatg atcaaaacgt caatattgaa tcaggagctt gtaaaaatga
    61981 caagacgggc aagcgcccgt cctgaatgat attacaaatt gtggaaacag cctaaaaatt
    62041 accagccttt aacagcgccg ccgttaaaca ctttgtttgc tgcttcgtaa acttcatcag
    62101 actgataagc ctggacgaat ttcttcacgt tctcggcgtc tttgttatct tcacgcgtta
    62161 cgatcaggtt tacgtacggg gagtctttat cttcgacaaa gataccgtct ttcgctggag
    62221 tcaggccaat ctggctggca taggtggtat tgataactgc cagagcgatt tgcgcgtcgt
    62281 ccagagatcg aggcagctgc ggtgcttcca gctcaacaat tttcagattt tttgggttct
    62341 caacaacatc aagaacggtc ggcagcaggc caacgccatc tttcagtttg atcaagccca
    62401 ctttttgcag cagcagcagt gaacgaccaa gattagttgg atcgtttggc acggcaacct
    62461 gcgaaccatc ctgcagttca tccagtgatt tgattttctt ggagtaacca gcaatcggat
    62521 aaacaaaagt gttgcctact gcgaccagtt tgtagccacg atctttcagt tgctgatcaa
    62581 ggtacggttt atgctggaag gcgttggcgt cgatatcgcc tttgctcaat gcttcgtttg
    62641 gcagaacata gtcgttgaag gttaccagct caacgtccag gccatatttg tctttcgcaa
    62701 ctttctgcgc gacttctgct acctgctgtt cggcaccaac aatcacgccg actttaatgt
    62761 ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc cagtgatcca atcagggctc
    62821 ccactgccgc aaaggttttg aatttgaacg ccatacctta ttccttcttc aattatttat
    62881 gttgtgttga acgttacttg cgagtgacag cccggacgat gcggtcgcct gcgaactgaa
    62941 ttaaataaac cagaatgacc agcaatacca gtaccgtatt catcaccgtc gcgttatagc
    63001 cgatgtagcc atactgatag ccaatctgac ctaaaccacc ggcaccgact gcaccaccca
    63061 tcgcggaata gccgaccagg gtaatcaggg tgatagttgc cgcattcacc agacccggca
    63121 gtgcttccgg taacagcacc ttacggacga tctgcatcgg cgtggcaccc attgcgcggg
    63181 aagcttcaat taacccggtt gggatctcca gcagagcgtt ctcgaccata cgggcaataa
    63241 acggtgctgc accaacggtt aacggaacaa tcgctgcctg caaaccaatc gatgtaccga
    63301 caataacgcg ggtaaacgga atcatccata ccagcaagat aatgaacggg atggaacgga
    63361 aaatgttcac aatcgcagaa acggtacgat acagcttcgc gttagcaata atttgccccg
    63421 ggcgcgtaac ataaagcagg acgccgaccg gcagaccaat cacaaagcca aaaaatccgg
    63481 atacgaaggt cattgccagc gtttcccata cgccacgaac cagcaaccac atcatcggct
    63541 cagacataac ccagtacctc tacttttaca tggtgttcct gcagccaggc aatggcggct
    63601 tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga tgccgaactt aacgccaccg
    63661 gcgtaatcca tctgcgcgct aataatgttg ttgttgacgt tgaaacgacg cgcggtttca
    63721 gaaagcagcg gggcatcgac cgattgaccg gtaaactcca ggcgcaacat cggcacgcag
    63781 tcagtaaatg gctccgcttg cagacgttcc tggtaatctt ccgggatatc cagatgcagg
    63841 gtcgactgaa taaacttctg cgccagcggc gttttcggat gcgagaacac ttcacttacc
    63901 gtgtcctgct cgatcagttc gccattgctg atgaccgcca cgcaatcaca aatacgcttc
    63961 acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac ccagacggcg gttaatgtct
    64021 ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca gcgcgctggt ggcttcatca
    64081 cacagcaata ctttgggatt gctggctaat gcacgggcaa tcgccacacg ttgtttctgc
    64141 ccaccggaaa gattcgacgg gtagctatca tgcttatcgc caagaccaac caatgacagc
    64201 aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt tgtccagctc cagcggcaga
    64261 gccacgttgc caaaaacagt acgcgaagag agcaggttaa aatgctggaa aatcatacca
    64321 atctggcggc gagctttggt caactcggat tctgacagcg tggtcagttc ctggccatcg
    64381 accagcacgc taccctcggt tgggcgctcc agcaggttta cacaacgtat aagcgtactc
    64441 ttacccgcgc ctgaggcacc gataacgcca taaatttgtc cagctggcac atgcaggctg
    64501 acgttgttca acgcctggat ggtgcgggtg ccctggtgga acactttggt gatattcgaa
    64561 agttttatca ttgattattt attatcgtca ttaagttagt cgtggcatct cgaatgcctg
    64621 aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc cagacgtcta aatcaatcag
    64681 gtttatgcga agagcacttt cttgcaggtc gaaacatgcg atactagcgt cccatgcctt
    64741 attaaggagc tataaaaggt ggcgaagagc gtacccgcaa tttttcttga ccgtgatggc
    64801 accattaatg tcgatcacgg ctatgtccat gagatcgaca actttgaatt tatcgacggt
    64861 gttattgacg ccatgcgcga gctaaaaaaa atgggctttg cgctggtggt agtaaccaac
    64921 cagtctggca ttgctcgcgg taaatttacc gaagcacagt ttgaaacgct gaccgagtgg
    64981 atggactggt cgctggcgga ccgagatgtc gatctggatg gtatctatta ttgcccgcat
    65041 catccgcagg gtagtgttga agagtttcgc caggtctgcg attgccgcaa accacatccg
    65101 gggatgcttt tgtcagcacg cgattatttg catattgata tggccgcttc ttatatggtg
    65161 ggcgataaat tagaagatat gcaggcagcg gttgcggcga acgtgggaac aaaagtgctg
    65221 gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg cggcagattg ggtgttaaat
    65281 agcctggcag acctgccgca agcgataaaa aagcagcaaa aaccggcgca atgattaaaa
    65341 gatgagcggt tgaaataaaa atgcattttt ccgcttgtct tcctgagccg actccctata
    65401 atgcgcctcc atcgacacgg cgaatgtgaa tcacttcaca caaacagccg gttcggttga
    65461 agagaaaaaa tcctgaaatt cagggttgac tctgaaagag gaatgcgtaa tatacgccac
    65521 ctcgcgacag tgagctgaaa gc
//
LOCUS       DAZFVO020000006        62952 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFVO020000006 DAZFVO020000000
VERSION     DAZFVO020000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 62952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.006"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKNB_RS01610"
                     /old_locus_tag="pgaptmp_000322"
     CDS             complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHKNB_RS01610"
                     /old_locus_tag="pgaptmp_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HGU4193904.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPI
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKNB_RS01615"
                     /old_locus_tag="pgaptmp_000323"
     CDS             complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHKNB_RS01615"
                     /old_locus_tag="pgaptmp_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HGU4193905.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKNB_RS01620"
                     /old_locus_tag="pgaptmp_000324"
     CDS             complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHKNB_RS01620"
                     /old_locus_tag="pgaptmp_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HGU4193906.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKNB_RS01625"
                     /old_locus_tag="pgaptmp_000325"
     CDS             complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHKNB_RS01625"
                     /old_locus_tag="pgaptmp_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HGU4193907.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDCLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYHDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKNB_RS01630"
                     /old_locus_tag="pgaptmp_000326"
     CDS             complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHKNB_RS01630"
                     /old_locus_tag="pgaptmp_000326"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGU4193908.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKNB_RS01635"
                     /old_locus_tag="pgaptmp_000327"
     CDS             complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHKNB_RS01635"
                     /old_locus_tag="pgaptmp_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGU4193909.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKNB_RS01640"
                     /old_locus_tag="pgaptmp_000328"
     CDS             complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHKNB_RS01640"
                     /old_locus_tag="pgaptmp_000328"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGU4193910.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKNB_RS01645"
                     /old_locus_tag="pgaptmp_000329"
     CDS             complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHKNB_RS01645"
                     /old_locus_tag="pgaptmp_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGU4193911.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKNB_RS01650"
                     /old_locus_tag="pgaptmp_000330"
     CDS             complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHKNB_RS01650"
                     /old_locus_tag="pgaptmp_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGU4193912.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKNB_RS01655"
                     /old_locus_tag="pgaptmp_000331"
     CDS             complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHKNB_RS01655"
                     /old_locus_tag="pgaptmp_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGU4193913.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKNB_RS01660"
                     /old_locus_tag="pgaptmp_000332"
     CDS             complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHKNB_RS01660"
                     /old_locus_tag="pgaptmp_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGU4193914.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13702..13778)
                     /locus_tag="ACHKNB_RS01665"
                     /old_locus_tag="pgaptmp_000333"
     tRNA            complement(13702..13778)
                     /locus_tag="ACHKNB_RS01665"
                     /old_locus_tag="pgaptmp_000333"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13742..13744),aa:Met,seq:cat)
     gene            14126..15469
                     /gene="argG"
                     /locus_tag="ACHKNB_RS01670"
                     /old_locus_tag="pgaptmp_000334"
     CDS             14126..15469
                     /gene="argG"
                     /locus_tag="ACHKNB_RS01670"
                     /old_locus_tag="pgaptmp_000334"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HGU4193915.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKNB_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /pseudo
     CDS             complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHKNB_RS01675"
                     /old_locus_tag="pgaptmp_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17560..17646)
                     /locus_tag="ACHKNB_RS01680"
                     /old_locus_tag="pgaptmp_000336"
     tRNA            complement(17560..17646)
                     /locus_tag="ACHKNB_RS01680"
                     /old_locus_tag="pgaptmp_000336"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17610..17612),aa:Leu,seq:gag)
     gene            complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKNB_RS01685"
                     /old_locus_tag="pgaptmp_000337"
     CDS             complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHKNB_RS01685"
                     /old_locus_tag="pgaptmp_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGU4193916.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKNB_RS01690"
                     /old_locus_tag="pgaptmp_000338"
     CDS             complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHKNB_RS01690"
                     /old_locus_tag="pgaptmp_000338"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGU4193917.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKNB_RS01695"
                     /old_locus_tag="pgaptmp_000339"
     CDS             complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHKNB_RS01695"
                     /old_locus_tag="pgaptmp_000339"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGU4193918.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKNB_RS01700"
                     /old_locus_tag="pgaptmp_000340"
     CDS             complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHKNB_RS01700"
                     /old_locus_tag="pgaptmp_000340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGU4193919.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKNB_RS01705"
                     /old_locus_tag="pgaptmp_000341"
     CDS             complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHKNB_RS01705"
                     /old_locus_tag="pgaptmp_000341"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HGU4193920.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKNB_RS01710"
                     /old_locus_tag="pgaptmp_000342"
     CDS             23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHKNB_RS01710"
                     /old_locus_tag="pgaptmp_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGU4193921.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKNB_RS01715"
                     /old_locus_tag="pgaptmp_000343"
     CDS             complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHKNB_RS01715"
                     /old_locus_tag="pgaptmp_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGU4193922.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKNB_RS01720"
                     /old_locus_tag="pgaptmp_000344"
     CDS             24451..25884
                     /gene="dacB"
                     /locus_tag="ACHKNB_RS01720"
                     /old_locus_tag="pgaptmp_000344"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4193923.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKNB_RS01725"
                     /old_locus_tag="pgaptmp_000345"
     CDS             complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHKNB_RS01725"
                     /old_locus_tag="pgaptmp_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HGU4193924.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKNB_RS01730"
                     /old_locus_tag="pgaptmp_000346"
     CDS             complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHKNB_RS01730"
                     /old_locus_tag="pgaptmp_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4193925.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKNB_RS01735"
                     /old_locus_tag="pgaptmp_000347"
     CDS             complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHKNB_RS01735"
                     /old_locus_tag="pgaptmp_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGU4193926.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKNB_RS01740"
                     /old_locus_tag="pgaptmp_000348"
     CDS             complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHKNB_RS01740"
                     /old_locus_tag="pgaptmp_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGU4193927.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKNB_RS01745"
                     /old_locus_tag="pgaptmp_000349"
     CDS             29052..30023
                     /gene="ispB"
                     /locus_tag="ACHKNB_RS01745"
                     /old_locus_tag="pgaptmp_000349"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HGU4193928.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKNB_RS01750"
                     /old_locus_tag="pgaptmp_000350"
     CDS             30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHKNB_RS01750"
                     /old_locus_tag="pgaptmp_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HGU4193929.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKNB_RS01755"
                     /old_locus_tag="pgaptmp_000351"
     CDS             complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHKNB_RS01755"
                     /old_locus_tag="pgaptmp_000351"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGU4193930.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKNB_RS01760"
                     /old_locus_tag="pgaptmp_000352"
     CDS             complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHKNB_RS01760"
                     /old_locus_tag="pgaptmp_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HGU4193931.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKNB_RS01765"
                     /old_locus_tag="pgaptmp_000353"
     CDS             complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHKNB_RS01765"
                     /old_locus_tag="pgaptmp_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HGU4193932.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKNB_RS01770"
                     /old_locus_tag="pgaptmp_000354"
     CDS             complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHKNB_RS01770"
                     /old_locus_tag="pgaptmp_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HGU4193933.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKNB_RS01775"
                     /old_locus_tag="pgaptmp_000355"
     CDS             complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHKNB_RS01775"
                     /old_locus_tag="pgaptmp_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HGU4193934.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKNB_RS01780"
                     /old_locus_tag="pgaptmp_000356"
     CDS             complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHKNB_RS01780"
                     /old_locus_tag="pgaptmp_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HGU4193935.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKNB_RS01785"
                     /old_locus_tag="pgaptmp_000357"
     CDS             complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHKNB_RS01785"
                     /old_locus_tag="pgaptmp_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HGU4193936.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHKNB_RS01790"
                     /old_locus_tag="pgaptmp_000358"
     CDS             35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHKNB_RS01790"
                     /old_locus_tag="pgaptmp_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HGU4193937.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHKNB_RS01795"
                     /old_locus_tag="pgaptmp_000359"
     CDS             36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHKNB_RS01795"
                     /old_locus_tag="pgaptmp_000359"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HGU4193938.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHKNB_RS01800"
                     /old_locus_tag="pgaptmp_000360"
     CDS             37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHKNB_RS01800"
                     /old_locus_tag="pgaptmp_000360"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HGU4193939.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38177..38752
                     /gene="lptC"
                     /locus_tag="ACHKNB_RS01805"
                     /old_locus_tag="pgaptmp_000361"
     CDS             38177..38752
                     /gene="lptC"
                     /locus_tag="ACHKNB_RS01805"
                     /old_locus_tag="pgaptmp_000361"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HGU4193940.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38721..39278
                     /gene="lptA"
                     /locus_tag="ACHKNB_RS01810"
                     /old_locus_tag="pgaptmp_000362"
     CDS             38721..39278
                     /gene="lptA"
                     /locus_tag="ACHKNB_RS01810"
                     /old_locus_tag="pgaptmp_000362"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HGU4193941.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39285..40010
                     /gene="lptB"
                     /locus_tag="ACHKNB_RS01815"
                     /old_locus_tag="pgaptmp_000363"
     CDS             39285..40010
                     /gene="lptB"
                     /locus_tag="ACHKNB_RS01815"
                     /old_locus_tag="pgaptmp_000363"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HGU4193942.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHKNB_RS01820"
                     /old_locus_tag="pgaptmp_000364"
     CDS             40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHKNB_RS01820"
                     /old_locus_tag="pgaptmp_000364"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HGU4193943.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41514..41801
                     /gene="hpf"
                     /locus_tag="ACHKNB_RS01825"
                     /old_locus_tag="pgaptmp_000365"
     CDS             41514..41801
                     /gene="hpf"
                     /locus_tag="ACHKNB_RS01825"
                     /old_locus_tag="pgaptmp_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HGU4193944.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHKNB_RS01830"
                     /old_locus_tag="pgaptmp_000366"
     CDS             41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHKNB_RS01830"
                     /old_locus_tag="pgaptmp_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HGU4193945.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHKNB_RS01835"
                     /old_locus_tag="pgaptmp_000367"
     CDS             42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHKNB_RS01835"
                     /old_locus_tag="pgaptmp_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGU4193946.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43307..43579
                     /gene="npr"
                     /locus_tag="ACHKNB_RS01840"
                     /old_locus_tag="pgaptmp_000368"
     CDS             43307..43579
                     /gene="npr"
                     /locus_tag="ACHKNB_RS01840"
                     /old_locus_tag="pgaptmp_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HGU4193947.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHKNB_RS01845"
                     /old_locus_tag="pgaptmp_000369"
     CDS             43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHKNB_RS01845"
                     /old_locus_tag="pgaptmp_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HGU4193948.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSGEA"
     gene            complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHKNB_RS01850"
                     /old_locus_tag="pgaptmp_000370"
     CDS             complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHKNB_RS01850"
                     /old_locus_tag="pgaptmp_000370"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HGU4193949.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHKNB_RS01855"
                     /old_locus_tag="pgaptmp_000371"
     CDS             complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHKNB_RS01855"
                     /old_locus_tag="pgaptmp_000371"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HGU4193950.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHKNB_RS01860"
                     /old_locus_tag="pgaptmp_000372"
     CDS             complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHKNB_RS01860"
                     /old_locus_tag="pgaptmp_000372"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HGU4193951.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGLVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHKNB_RS01865"
                     /old_locus_tag="pgaptmp_000373"
     CDS             complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHKNB_RS01865"
                     /old_locus_tag="pgaptmp_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HGU4193952.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFARYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            50065..54525
                     /gene="gltB"
                     /locus_tag="ACHKNB_RS01870"
                     /old_locus_tag="pgaptmp_000374"
     CDS             50065..54525
                     /gene="gltB"
                     /locus_tag="ACHKNB_RS01870"
                     /old_locus_tag="pgaptmp_000374"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HGU4193953.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEALGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            54538..55956
                     /gene="gltD"
                     /locus_tag="ACHKNB_RS01875"
                     /old_locus_tag="pgaptmp_000375"
     CDS             54538..55956
                     /gene="gltD"
                     /locus_tag="ACHKNB_RS01875"
                     /old_locus_tag="pgaptmp_000375"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HGU4193954.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            56140..57267
                     /gene="yhcG"
                     /locus_tag="ACHKNB_RS01880"
                     /old_locus_tag="pgaptmp_000376"
     CDS             56140..57267
                     /gene="yhcG"
                     /locus_tag="ACHKNB_RS01880"
                     /old_locus_tag="pgaptmp_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HGU4193955.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(57327..57791)
                     /gene="nanQ"
                     /locus_tag="ACHKNB_RS01885"
                     /old_locus_tag="pgaptmp_000377"
     CDS             complement(57327..57791)
                     /gene="nanQ"
                     /locus_tag="ACHKNB_RS01885"
                     /old_locus_tag="pgaptmp_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HGU4193956.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(57788..58663)
                     /gene="nanK"
                     /locus_tag="ACHKNB_RS01890"
                     /old_locus_tag="pgaptmp_000378"
     CDS             complement(57788..58663)
                     /gene="nanK"
                     /locus_tag="ACHKNB_RS01890"
                     /old_locus_tag="pgaptmp_000378"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4193957.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(58660..59349)
                     /gene="nanE"
                     /locus_tag="ACHKNB_RS01895"
                     /old_locus_tag="pgaptmp_000379"
     CDS             complement(58660..59349)
                     /gene="nanE"
                     /locus_tag="ACHKNB_RS01895"
                     /old_locus_tag="pgaptmp_000379"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGU4193958.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(59397..60887)
                     /gene="nanT"
                     /locus_tag="ACHKNB_RS01900"
                     /old_locus_tag="pgaptmp_000380"
     CDS             complement(59397..60887)
                     /gene="nanT"
                     /locus_tag="ACHKNB_RS01900"
                     /old_locus_tag="pgaptmp_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HGU4193959.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(60997..61890)
                     /gene="nanA"
                     /locus_tag="ACHKNB_RS01905"
                     /old_locus_tag="pgaptmp_000381"
     CDS             complement(60997..61890)
                     /gene="nanA"
                     /locus_tag="ACHKNB_RS01905"
                     /old_locus_tag="pgaptmp_000381"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4193960.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(62012..62803)
                     /gene="nanR"
                     /locus_tag="ACHKNB_RS01910"
                     /old_locus_tag="pgaptmp_000382"
     CDS             complement(62012..62803)
                     /gene="nanR"
                     /locus_tag="ACHKNB_RS01910"
                     /old_locus_tag="pgaptmp_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4193961.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        15392 a        17111 c        15551 g        14898 t
ORIGIN      
        1 gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg
       61 attactgata caccggcagt aaattaaagc tcgataaaat atgcaccagt gcgttgccga
      121 cgccaaacac cagaatcagc gcaatcatcg gcttgccacc ccagacgcgg aatttcgggc
      181 tgccaaagcg tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg
      241 tagctgctaa accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac
      301 ccacaatagg cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat
      361 cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac
      421 tcgctaccgc aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca
      481 cgccgcttaa tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa
      541 actccggacg cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg
      601 ccatcagcgt accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact
      661 tcatcaggct tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca
      721 tcagcagata cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct
      781 gcacatgccc caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca
      841 caatcgccgt catgcggctg acggccttag tactcaacca caccacaaac gctaccagca
      901 gtgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt
      961 gatgcagaat tgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg
     1021 caatggaaat accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt
     1081 caaaactcga accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca
     1141 gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca
     1201 tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc
     1261 cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc
     1321 agtgaaaaat agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa
     1381 agacagaaaa aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt
     1441 gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat
     1501 cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc
     1561 caccttcacg gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac
     1621 cacgaccacc gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca
     1681 tgttcatcgg cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca
     1741 tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac
     1801 ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat
     1861 catcgcggcc cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac
     1921 gcggacgatc ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt
     1981 catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag
     2041 tacgttcacc ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt
     2101 caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc
     2161 tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt
     2221 ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca
     2281 gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac
     2341 cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta
     2401 ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt
     2461 ccagacgacc atctttcagg cgttccagtg tctgctcacg cagcgcctgg ttcatgtcac
     2521 cgttcagcgc ggcactgttg tagccgttac gctcaagagc ttcagccact tccagagtcg
     2581 catttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta
     2641 ccagtgcttc gtttttacgc ataccccaga cagtccagta gctctggctg atgtcaggac
     2701 gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa
     2761 tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga
     2821 tctgcgccat aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt
     2881 catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca
     2941 gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca
     3001 cgtcataacg ctggccgccg tatagagcaa ccacatttac gccgcgcatg tgtttagaga
     3061 aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa
     3121 tctgtggtgc tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag
     3181 tttttccgct ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa
     3241 tacactctgc ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga
     3301 taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt
     3361 gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc
     3421 attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg
     3481 ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc
     3541 cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg
     3601 gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct
     3661 gtccaaatcc cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg
     3721 ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc
     3781 gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt
     3841 ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag
     3901 ataaagccac agactacgga aaggatcatt gggatcgtcg tgataaaacg ccagcagatc
     3961 atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc
     4021 gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa
     4081 attgcctgcc tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg
     4141 gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gacaatcata
     4201 caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc
     4261 aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac
     4321 cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt
     4381 cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa
     4441 tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc
     4501 agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc
     4561 agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc
     4621 aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc
     4681 aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc
     4741 aacaaatgcg ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc
     4801 gatttctgca gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt
     4861 cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt
     4921 cagggcacgg ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat
     4981 cttgatggta tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc
     5041 ctgttccatt acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg
     5101 catgatctct ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc
     5161 gcgggaacct gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc
     5221 agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc
     5281 agctttgatt ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat
     5341 agaagaagaa ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt
     5401 gtccatatcc ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc
     5461 acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta
     5521 gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg
     5581 agcggtaccc agcgttgcag tgaccagtgc ctgcgtttca ccgcgggtga acagcgcaga
     5641 accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc
     5701 acgaccgtcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat
     5761 agcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt
     5821 cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat
     5881 gcggtaagca tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc
     5941 gtttaccggc tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt
     6001 gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg
     6061 gtcttcgctc agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc
     6121 aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg
     6181 gtcattgatg taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag
     6241 agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac
     6301 agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg
     6361 aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg
     6421 acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt
     6481 cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac
     6541 cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt
     6601 ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt
     6661 aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct
     6721 gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt
     6781 agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg
     6841 aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa
     6901 gaattagcga cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg
     6961 tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct
     7021 gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag
     7081 cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac
     7141 gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga
     7201 aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtt ttaaacaatt
     7261 tacgcgacgt taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac
     7321 attacgccgg gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt
     7381 cgcccatacc gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg
     7441 gcgcaccgga tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa
     7501 gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta
     7561 actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca
     7621 gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat
     7681 gcgcgccaca accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt
     7741 ttgagcagtg aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat
     7801 aaacggtaat cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca
     7861 gttttttacc ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac
     7921 ggaaagtatc cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa
     7981 cgatttgtcc gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat
     8041 agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg
     8101 gcaacatgcc agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat
     8161 aaatccgttt cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca
     8221 acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct
     8281 gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga
     8341 catgcgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg
     8401 caggcgcatc gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt
     8461 gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc
     8521 atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg
     8581 atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg
     8641 ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag
     8701 ccccttttta aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc
     8761 cagcgtcaca tcaggcaacg gctgccggat gcggcgtaaa agccttatcc ggcctacaac
     8821 ccagacaacc ttaagcgatg gtacgttgga tttcgatgat ctcgaatact tcgatcacat
     8881 cgccagtgcg gacatcgttg tagttcttaa cgccgatacc acattccata ccgttacgga
     8941 cttcgttaac gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca
     9001 cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac
     9061 agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga
     9121 taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa
     9181 tcaggttata gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac
     9241 gtgcagaggc atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca
     9301 gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt
     9361 cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg
     9421 ccttcaggac gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct
     9481 cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca
     9541 gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg
     9601 gtacgccgga caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct
     9661 gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat
     9721 cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat
     9781 cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca
     9841 gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gatgccggta cccgctttcg
     9901 cagatacgtg tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccatactggg
     9961 agagttcgtt cttaacacga tccggatcgg cttctggttt atcaatcttg ttcactgcaa
    10021 ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatca
    10081 cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgtgcac
    10141 gcattgaagt aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag
    10201 tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggcaactt
    10261 tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg
    10321 tcacaaccgg cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct
    10381 cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct
    10441 gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg
    10501 ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatgg
    10561 tttcgccgat cacaacgtca cggttaacgg cctgagctgg cttctggaag ccttgctgca
    10621 gggaagaacc tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac
    10681 gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac
    10741 gaccacggcc acgaccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat
    10801 gttgagaagt agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc
    10861 atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga
    10921 gtttacgacg cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc
    10981 gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt
    11041 tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcagcct
    11101 cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt
    11161 gctcttccgc ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat
    11221 cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac
    11281 cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc
    11341 ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt
    11401 cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg
    11461 tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta
    11521 cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc
    11581 cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga
    11641 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc
    11701 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
    11761 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
    11821 caaggccttc gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg
    11881 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
    11941 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
    12001 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
    12061 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
    12121 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt
    12181 tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa
    12241 cacgcgcgcc acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt
    12301 tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt
    12361 cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac
    12421 gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa
    12481 cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt
    12541 cagcattgtt gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg
    12601 tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac
    12661 gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag
    12721 actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg
    12781 cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga
    12841 aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat
    12901 attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag
    12961 gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg
    13021 gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac
    13081 tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg
    13141 ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca
    13201 cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg
    13261 gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac
    13321 ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat
    13381 agatgcgcag tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca
    13441 gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca
    13501 cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa
    13561 ccccgataaa tcggggcttt atataactga accctataac cgcaactgcg gtctggagca
    13621 ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg
    13681 aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt
    13741 tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac
    13801 tcgttttggg taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa
    13861 atctgcaggc attatagtga tccacgccac attttgtcaa cgtttattgc taatcatgtg
    13921 aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg ctccccctta
    13981 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
    14041 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
    14101 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
    14161 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
    14221 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
    14281 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
    14341 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
    14401 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
    14461 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
    14521 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
    14581 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
    14641 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
    14701 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
    14761 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
    14821 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
    14881 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
    14941 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
    15001 cgaggctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca
    15061 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
    15121 ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt
    15181 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
    15241 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
    15301 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat
    15361 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
    15421 cacttcaggc gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa
    15481 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
    15541 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
    15601 atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata
    15661 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc
    15721 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
    15781 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
    15841 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
    15901 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
    15961 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
    16021 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
    16081 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
    16141 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
    16201 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
    16261 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta
    16321 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
    16381 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
    16441 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
    16501 tgatgctctt tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca
    16561 aatcgcgacg ctagtatata tggactgaac gcttttttat tttttgcatc tttataagca
    16621 aattgacaag cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
    16681 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
    16741 aagcaaaggc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
    16801 cactctggag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
    16861 aatagaggct aatactcatg tctgccgtag tcagtaacac aaatggtgcg gcaacaagca
    16921 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
    16981 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
    17041 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
    17101 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
    17161 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
    17221 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
    17281 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
    17341 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
    17401 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
    17461 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
    17521 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
    17581 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
    17641 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
    17701 ggttgagttt gttcggtttt caccggtgca ctcagatttt cccattcgct acctttattg
    17761 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
    17821 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
    17881 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
    17941 ccaacaaggc caattgccac aataaggaaa actactaaaa gagcttcgta cataatcaac
    18001 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
    18061 ctttcccact gaagcgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
    18121 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
    18181 aggcgacaat cgccgccttt ttagccagtt atctaacgct ttaaacggct tttactgcat
    18241 cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc
    18301 gaattaacgg ttcggtgccg gatttacgca gcaacacgcg tccacggttg cccagcgcag
    18361 cttcaacctc tgcggtcacg gctttaactg actcatgctc aagtggatcg ccgctacctg
    18421 cggtgtaacg tacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
    18481 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
    18541 caccggtagt agttttatcc agtaggatca catgaccgga attctctgca ccgatacgcc
    18601 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
    18661 gaattcctaa ctgtttcagc gccagttcaa gccccatgtt gctcatcaat gtacccacag
    18721 cgccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
    18781 cgccatcgac tttattgcct tcatggtcga ccataatcac gcgatcgcca tcgccgtcga
    18841 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
    18901 tagcccccac ttcggcattg atgtttacac cgtttggctc acaaccgata gcgataacgt
    18961 tcgcccccag ttcgcgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcacaat
    19021 ccaccacaat cttcagttca ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
    19081 cgatatagcg acccgcggca tcaacgatac ggctggcttt acccagttct gccgaatcaa
    19141 cgcagctgat ctccttttcc atttccgctt cgatggcctc ttctaccgca tccggcagtt
    19201 tggtgccgtc gatagagaag aatttaatcc cattatcgta gaacgggtta tgcgatgcag
    19261 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
    19321 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
    19381 actccagcat atagccagaa atacgcgtgt ctttaccaat aataatctta cgggagccgt
    19441 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
    19501 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
    19561 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
    19621 ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
    19681 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
    19741 cgtgacatac ccaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
    19801 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
    19861 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
    19921 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
    19981 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
    20041 agggagcgga tatcattaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
    20101 ggtttggatg tatcgaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
    20161 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
    20221 tcgccaccaa cgtcaatgat cgtcgcgcca gcattgatca tcagattcgc atgtttcacc
    20281 gcatctatca gcgagttatg cgtgccgcca tccgaaaagg aatcaggcgt gacgttgagg
    20341 atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcatg
    20401 atgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
    20461 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
    20521 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
    20581 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
    20641 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
    20701 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
    20761 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
    20821 tcgctttcgc tacgctacgg ccgaggaata cttcaccttc ttcttccgcg tacagcagtg
    20881 gacccaattt ctcagagaag ccccactggg tcaccatgtt acgtgccaga ttggtcgcaa
    20941 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccatagatg atctcttctg
    21001 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
    21061 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
    21121 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
    21181 cgtggtaagc cgttgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
    21241 ccatcatgat tttgtctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
    21301 cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc caggtcagca ccggagaaac
    21361 caggagtacc acgggcaatg attgccgcgt cgatatcggg ggccaatggt acgcgacgca
    21421 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccgacc acaacctgac
    21481 ggtcgaaacg gccaggacgc agcagggccg ggtcgagaac gtccggacgg ttagtcgcgg
    21541 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
    21601 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
    21661 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
    21721 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
    21781 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac
    21841 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
    21901 tcggctcgcg cagatactct accagttcag caacttcttc ttttgcttcg tcgcagcccg
    21961 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
    22021 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaga
    22081 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
    22141 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
    22201 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
    22261 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
    22321 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
    22381 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
    22441 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
    22501 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
    22561 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
    22621 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
    22681 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
    22741 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
    22801 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
    22861 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
    22921 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
    22981 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
    23041 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
    23101 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
    23161 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
    23221 tatgggagat ggcggtagaa tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg
    23281 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
    23341 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
    23401 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac taaaaccttg
    23461 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
    23521 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
    23581 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
    23641 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
    23701 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
    23761 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
    23821 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
    23881 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
    23941 aaaataacgc gcccattgtt gggcattttg gtgacatcaa tcacctgcgc gttcgacagc
    24001 ttggcttcga tgtctttaat acggccttcg cagaaaccct gctgttcacg agctgcgtgg
    24061 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    24121 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    24181 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    24241 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    24301 aaaataccga cccgggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    24361 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    24421 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    24481 accagctgta tagcgttcag tgttcaggcc gcgaatgttg atgagtacgt tactcaactc
    24541 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    24601 gattaccaca gtcagcagat ggcgctgccc gccagtacgc agaaagtgat tactgcgctg
    24661 gcggcgttga ttcaactcgg ccccgatttt cgtttcacca cgacgcttga aaccaaaggc
    24721 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    24781 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    24841 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    24901 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgctgc catagttgac
    24961 cgcaactgtt tctccgtctc gctctacagt gccccaaaac ctggtgatat ggcttttata
    25021 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgaggt
    25081 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    25141 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    25201 ggagccagct atgccggggc aattctgaaa gatgagttaa aacaggcagg tatcacctgg
    25261 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    25321 cagtcggccc cgttgcacga tctgcttaag attatgctga aaaagtcgga caacatgatc
    25381 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg aacatggcgg
    25441 gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    25501 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    25561 atgcaggtgt tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    25621 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    25681 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    25741 attaccacag cgagcgggca acgaatggcg tttgtacaat atctttctgg ctatgcagta
    25801 gaaccggcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    25861 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggttttgc
    25921 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcatcatc
    25981 ccagtcctca tcatcctctt cagcaatctc ttcaagctgc tggcgatgat aatcatccca
    26041 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    26101 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccactcg ccgcagagat
    26161 cagataatat ttatcttccc agcccagcgc ttcagcaatc gctttcgctt tctcttcagc
    26221 ttctgcctta tccagcagat cgatcttatt gaacactaac caacgcggtt tcgccgccag
    26281 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    26341 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    26401 caggaagcgg atacccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    26461 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    26521 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcgcgaa taaaggtcga
    26581 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    26641 cagctcacgc ttatcacccg gcgtaccgtt ggttttctgc cgtggtgtac ggttaacgga
    26701 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    26761 accgtgttta gtcatatcgc ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    26821 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    26881 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    26941 gttttcgtcg gcttccatcc atacgtcacc accatcacca ccgtcgccgc catccgggcc
    27001 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    27061 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    27121 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    27181 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    27241 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    27301 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    27361 cgcttccgcc agggcgccat atcctaccaa tgtattcagt ccgcaaaaaa ttaaacatgc
    27421 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcaat
    27481 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    27541 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    27601 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    27661 caggccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    27721 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    27781 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    27841 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    27901 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    27961 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    28021 ccagcaaatt gctgtggtga gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    28081 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    28141 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    28201 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    28261 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    28321 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    28381 accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    28441 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    28501 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    28561 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    28621 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    28681 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    28741 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    28801 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    28861 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    28921 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa tcactctata
    28981 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    29041 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    29101 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca gctgatcaat cagttaggct
    29161 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    29221 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    29281 acacggcgac tctgctgcac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    29341 ctaccgccaa cgctgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    29401 cccgcgcgtt ccagatgatg accagcctcg gttcgctcaa agtgctggaa gtcatgtcag
    29461 aagccgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    29521 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    29581 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaagaggag aaaggcctgc
    29641 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    29701 atgccgatgg cgaacagtta ggtaaaaatg tcggcgacga tctgaacgaa ggtaaaccga
    29761 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    29821 ccgccatcga acagggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    29881 cttgtggatc tcttgaatgg acgcgtcagc gtgccgagga agaagcagac aaagccatcg
    29941 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    30001 tcgctgttca acgcgatcgt taatcccctc ccctcatccc gcgcagcgcg cggggtggtt
    30061 ccagtaaatt ccataaaaac acttactcag ctctataaca cctgaaaaac aggctgtgca
    30121 tctttatata ttctgaatat tcacactcct tacaggaact ttttagagca atcggccatc
    30181 aggagtatag tgatgctcga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    30241 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcgggtttg
    30301 cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag ttcctcaacg
    30361 ctggcgaatg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    30421 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    30481 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    30541 tagccccgcg aacggggctg ccagctctca gacgattatt cgcctttcac acgctcaata
    30601 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    30661 atacgatcaa ccaccgtcgt cccttccgca atacatccag ccagcaccag gcttgctgat
    30721 gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata
    30781 acggtattgc tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata
    30841 aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac
    30901 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    30961 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    31021 tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta
    31081 cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata
    31141 cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    31201 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    31261 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca
    31321 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    31381 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    31441 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    31501 ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa
    31561 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    31621 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat
    31681 gtatcgacgt ctttcagttt tgggacgttc tggatctcta ccggttcttc cgccagtaga
    31741 gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    31801 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    31861 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    31921 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    31981 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac
    32041 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    32101 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    32161 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    32221 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    32281 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    32341 ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt
    32401 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    32461 cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt
    32521 agcaaaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    32581 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    32641 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    32701 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg
    32761 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcggatcaa
    32821 taatggtaac gcgaatcggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    32881 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    32941 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    33001 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    33061 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    33121 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    33181 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    33241 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    33301 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    33361 caatgagatc ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca
    33421 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc
    33481 ccagcaggcc ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt
    33541 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    33601 cccgacccac aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt
    33661 tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg
    33721 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    33781 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    33841 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    33901 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    33961 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    34021 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat
    34081 ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca
    34141 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    34201 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc
    34261 ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    34321 agcataccca gactggtttc cgcactataa gtggtcagaa ccagatagcc ttgcagcccc
    34381 aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca
    34441 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    34501 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    34561 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    34621 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    34681 ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat
    34741 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    34801 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    34861 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    34921 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    34981 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    35041 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    35101 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac
    35161 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    35221 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    35281 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    35341 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    35401 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    35461 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    35521 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    35581 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    35641 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    35701 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    35761 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    35821 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    35881 cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta cccttaatgt
    35941 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg
    36001 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    36061 aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg
    36121 acggcggatt gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg
    36181 ccacgcggat ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt
    36241 tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa
    36301 tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca
    36361 tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc
    36421 cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc
    36481 tgtgctggcg gcgctcccca caaccgggcc gtggtgtagg ggtattatta actggcggat
    36541 ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa
    36601 cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa
    36661 gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc
    36721 acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg
    36781 aaatcggggc acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca
    36841 tttttcgtcc atcctggtga agccgcgcat ggtgatttag gcatggtcac cccacaggat
    36901 gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg
    36961 cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg
    37021 cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg
    37081 gcaccgacca gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg
    37141 ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg
    37201 ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat
    37261 gttaagaaaa cggccagtct gcgtgacgcg ttgctggaag ttacccgcaa aaatcttggt
    37321 atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta
    37381 cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg
    37441 ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag
    37501 tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat
    37561 atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca
    37621 aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atagcaaaag
    37681 cagagaacat tcgtctgctg atcctcgatg tcgatggcgt actgtcagat ggcctgattt
    37741 atatgggcaa taatggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc
    37801 gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg
    37861 tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaataaac
    37921 tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc cccggaaaat gtggcttatg
    37981 tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg
    38041 ccgatgcgca tccactgttg atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc
    38101 gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag
    38161 ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc
    38221 ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt
    38281 caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga
    38341 aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt
    38401 ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc
    38461 cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt
    38521 tgaagtcaac gcacttgtgc cagactctca acttcgcaga atcacgacgg ataacgcgca
    38581 gatcaatctg gtgactcagg atgttacctc tgaagacctc gtcacgttat acggaacaac
    38641 atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat
    38701 tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc
    38761 ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc
    38821 agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct
    38881 ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg
    38941 ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa
    39001 cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact
    39061 acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata
    39121 gcaacattaa gggcgataag atcacttacc tggtaaaaga gcagaaaatg caggctttca
    39181 gcgacaaagg caagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca
    39241 acaaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg
    39301 caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg
    39361 tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt
    39421 tctatatggt tgttggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg
    39481 atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg
    39541 aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc
    39601 gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc
    39661 acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg
    39721 tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg
    39781 ccggggttga cccgatctcg gttatcgaca ttaaacgcat cattgagcac ctgcgcgaca
    39841 gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac
    39901 gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac
    39961 aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa
    40021 gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag
    40081 gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt
    40141 gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga
    40201 gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga
    40261 caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg
    40321 ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga
    40381 gctgccggtc tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt
    40441 cgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt
    40501 tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga
    40561 agagatcgac atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt
    40621 cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa
    40681 gaccacgccg tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc
    40741 caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga
    40801 agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga
    40861 acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga
    40921 actcaacagt gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa
    40981 taacgcgcgc aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg
    41041 gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt
    41101 tgaacagcag caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc
    41161 cgatatcgcc caggctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa
    41221 atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa
    41281 taccgagggc ggcggcgaag cttcctccac ggcgattcgt gcgctggtga agaaattaat
    41341 cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga
    41401 acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc
    41461 gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc
    41521 tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcca
    41581 aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag
    41641 tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg
    41701 ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac
    41761 gccagttgac caaacataaa gataaactga aacaacacta attgtccggg caattagcat
    41821 gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct
    41881 gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc
    41941 tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca
    42001 aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc
    42061 aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg
    42121 gtattgccat tccgcatggc aaactggaag aagatactct gcgtgccgtt ggcgttttcg
    42181 ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt
    42241 ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg
    42301 cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag
    42361 agctgtatca aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta
    42421 atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac
    42481 gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg
    42541 tggataacct tcccgtagtg ttgttacccg atctggctcg aaccctggcc gatcgcgaga
    42601 tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac
    42661 aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc
    42721 gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa
    42781 acctgtcgct ggaaagtgct atcgacaaag aaagtgatct gctggagcct ctgcgttcgc
    42841 gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggca gaaatgctgc
    42901 gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat ggtctttgag tctttcggct
    42961 tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc
    43021 cgcactggga tccgaaactg cgtccaatga caggtcttga taagcctgtc gccgcgttcc
    43081 tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat
    43141 ggttacctat gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg
    43201 gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg
    43261 gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca
    43321 aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga
    43381 attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggctga
    43441 agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt
    43501 tgaagcgacc ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc
    43561 tggttttgat gaagattaat cttcatcact ttgacattca aatcccttca agctcccccg
    43621 gcaataagaa aagcgcgtac tgaaatcgtt ctcaatcaac gtcaattgta catttcgtgc
    43681 gctttttact ttcagaagaa ccttaagaaa accttaagaa gcattgttta ggtttcgttt
    43741 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    43801 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    43861 gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc
    43921 cgccgtgagt taaagtatta cgcgcatctt ggtcgccggt taaaagactg gagtggaata
    43981 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    44041 gccgattttg acggcaagcc ttccattacg ctgaccgaat ttgctgaaca atgccgttac
    44101 gaagaagaca ttgcacaact gcgccagtta ctgaaacagc tgaagcgtta tttgcaggat
    44161 aaccgtatcg tgacgatgtc gttgaagccg cagaatatcc tctgtcaccg cattagtgag
    44221 tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    44281 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttattgcc
    44341 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    44401 gccctcactt cgggcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct
    44461 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgcg
    44521 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgatcgg
    44581 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    44641 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagacc
    44701 gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct acgcccatcc
    44761 cataaaaaga gatttttggc cgtctgctga gaaatcgttg atgcaccgcg aatacggttt
    44821 tcattgcgct cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccagtgc
    44881 tcaggaaatt tctgatcttc tgcggcaatc accgccagtc ccatccatgg cgagatttga
    44941 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    45001 acctgtcgct cgaccattac cgctgagaag ggaacaggcg caacgctaaa caacgcgatg
    45061 cccccgcccc agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa
    45121 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    45181 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga
    45241 caatcttatt atcttcatca accacgatat catcgacagg acacggcaca tgctccgcgc
    45301 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga
    45361 aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg
    45421 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    45481 gagtggcaaa attgcttaaa ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat
    45541 ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg
    45601 cttcaatcag cacgttgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat
    45661 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    45721 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    45781 ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt
    45841 tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    45901 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    45961 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    46021 agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga
    46081 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcaggaagg
    46141 tccggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    46201 gcacctttaa ttttatgtcc ttcctctaca atgccttttt tatcctgcgc cgtcagattc
    46261 gactccagca cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg
    46321 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    46381 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    46441 aatttcttga tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc
    46501 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    46561 ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc
    46621 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    46681 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa
    46741 cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    46801 ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc
    46861 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    46921 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    46981 ccatgactgt ctttcacctg gtaatacatg gcgaaaattt tatccagctc atcctgcgga
    47041 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    47101 cgcacggtaa ccaggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg
    47161 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    47221 aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    47281 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    47341 tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatggtc
    47401 ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatacg gctcaggccg
    47461 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    47521 tcgcggctgg cccgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    47581 aagcccatca aaccgtggcg tttacccacg cggtcgtagt acggcacttt acggatttca
    47641 aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta
    47701 tgacggaaca ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca
    47761 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    47821 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca
    47881 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    47941 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    48001 cgaacggcga tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    48061 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac
    48121 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    48181 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt
    48241 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    48301 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    48361 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    48421 ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtatcc
    48481 acggacgttc cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga
    48541 ccattcccgt ccatcgattt tcgcaccaca gaggagcaag cagcgtcggg cgacgggcgc
    48601 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    48661 tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca
    48721 taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    48781 ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg ccgacaatta
    48841 agtgggaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacggg
    48901 caaaatcatg gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    48961 gctccagcca cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat
    49021 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    49081 cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa
    49141 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    49201 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    49261 caccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgt agcgtcagct
    49321 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    49381 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    49441 tcaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    49501 tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgtttat cttgtgtcag
    49561 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat
    49621 taaggcagta taaaatgctg gttttctcgt cagttcaagg caggataagg gttaacacgc
    49681 ctttatgaca gtcaggaatt gactgtttct ctaacgactt cccttttagc cttaaagata
    49741 aaatccattt taatttcagt aatttaataa agaattttgc gctaaagcac atttctgtac
    49801 caataagctt gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag
    49861 ctttctggat gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc
    49921 gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc
    49981 aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg
    50041 cagagcctgg gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt
    50101 ggtttcggcc tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca
    50161 atacacgcac tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc
    50221 gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag
    50281 cgcggctggc gtttagcaaa aaactacgct gtcgggatgc tcttcctgaa taaagatcct
    50341 gaactcgccg ctgccgcacg ccgcatcgtt gaagaagagc tgcaacgcga aaccttgtcg
    50401 attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc
    50461 tctctgcccc acattgagca aattttcgtt aacgctccgg caggctggcg tccgcgcgat
    50521 atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa
    50581 gacttctacg tctgtagcct gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg
    50641 gcggatctgc cgcgctttta tctggatctt gcggacctgc gtctggaatc ggccatctgc
    50701 ctgttccacc agcgcttctc cactaacacc gtaccgcgct ggccgctggc gcagccgttc
    50761 cgctacctgg cgcataacgg tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt
    50821 gcgcgtacct ataaattcca gacgccgctg atccctgatc tgcacgacgc cgcaccgttc
    50881 gtcaacgaaa ccggttctga ctccagttcg atggataaca tgctggaact gctgctggca
    50941 ggcgggatgg atatcatccg cgccatgcgt ctattagtac cacccgcctg gcagaacaac
    51001 ccggatatgg acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg
    51061 tgggatggcc cggcgggcat cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac
    51121 cgtaacggtc tgcgtccggc acgctacgtc attaccaaag acaagctcat cacctgcgcc
    51181 tctgaagtcg gtatctggga ttatcagcct gacgaagtgg tcgaaaaagg ccgcgtcggg
    51241 ccaggcgaac tgatggtgat cgacacccgc agtgggcgta ttctgcactc ggcagaaacc
    51301 gatgacgatc tgaaaagccg ccatccatat aaagagtgga tggagaaaaa cgtccgccga
    51361 ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta gccgcgaact ggacgacgac
    51421 acgcttgcca gctaccagaa acagtttaac tacagcgcgg aagagctgga ctccgtaatt
    51481 cgcgtactgg gcgaaaacgg tcaggaagcg ctcggttcga tgggcgatga taccccattc
    51541 gccgtgctct ccagtcagcc gcgcattatt tacgactact tccgccagca gtttgcccag
    51601 gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt
    51661 atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt
    51721 aaatcgccga ttctgctcta ctccgatttc aaacagctca cgacgatgaa agaggagcac
    51781 taccgcgcag atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgaca
    51841 gtcaaagagc tgtgcgacaa agccgaaaaa atggtacgta gcggcaccgt gctgctggtg
    51901 ctctccgacc ggaatatcgc taaagatcgc ctgccggttc cagccccgat ggcggttggc
    51961 gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa
    52021 accgccagcg cccgcgatcc gcaccacttc gccgtgttgc tgggcttcgg cgcgacggct
    52081 atttatccat accttgccta tgaaacgctg ggccgcctgg tagacaccca tgcgattgcc
    52141 aaagattatc gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa
    52201 atcatgtcca aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa
    52261 gcggtcggtc tgcacgatga tgtagtgggc ctgtgcttcc agggggcggt cagccgcatt
    52321 ggtggggcga gctttgaaga cttccagcag gatctgctga acctgtcgaa acgtgcctgg
    52381 ctggcgcgta agcccatcag ccagggcggc ctgctgaaat acgtccacgg cggcgaatac
    52441 cacgcttaca acccggacgt ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac
    52501 agcgactatc aggaatacgc gaagctggtt aatgagcgtc cggcaaccac gctgcgcgat
    52561 ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc
    52621 gaactgttta aacgctttga taccgccgcg atgtctatcg gcgcgttaag cccggaagcc
    52681 cacgaggcgc tggcggaagc gatgaatagc atcggcggta actcgaactc cggtgaaggc
    52741 ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc gcatcaagca ggtggcttct
    52801 ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg acgtcattca gattaaagtc
    52861 gcccagggcg cgaagccggg cgaaggcggt cagttgccgg gcgacaaagt cactccttac
    52921 atcgccaaac tgcgctattc ggtgcccgga gtgacgctga tctccccgcc gccgcaccac
    52981 gatatctact ctatcgagga cttagcgcag ctcattttcg acctcaagca ggttaacccg
    53041 aaagcgatga tctccgtgaa gctggtttcc gaaccgggcg taggcaccat cgcgactggc
    53101 gtggcaaaag cttatgccga tttaatcacc atcgcaggct atgacggtgg caccggcgca
    53161 agcccgctgt cgtcagtgaa atacgcaggt tgcccgtggg agctggggct tgttgaaacc
    53221 cagcaggcgc tggttgccaa tggtctgcgt cataagatcc gtttgcaggt cgatggcggc
    53281 ctgaaaaccg gtgttgatat catcaaagcg gcgattctcg gcgcagaaag cttcggcttc
    53341 ggcactggcc cgatggtggc gctcggctgt aaatatctac gtatttgcca tctgaacaac
    53401 tgcgcaacgg gtgtagcaac tcaggatgac aaactgcgta agaaccacta tcacggcctg
    53461 ccgttcaagg ttaccaatta ctttgaattt attgcccgtg aaacccgcga gctgatggca
    53521 cagctgggcg taacgcgtct ggtggatctg attggtcgca ccgacctgct gaaagaactg
    53581 gacggtttca ccgccaaaca gcaaaaactg gcgctgtcga agctgctgga gaccgccgaa
    53641 ccgcatccag gtaaggcact ctactgcacc gaaaacaacc caccgtttga taacggcctg
    53701 ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc
    53761 ttctggttcg atattcgcaa caccgaccgt tctgtcggcg cgtcgctttc aggctatatc
    53821 gcccagacgc acggcgatca ggggctggca gccgatccta tcaaagcgta cttcaacggc
    53881 accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg tggaactgta cctgaccggg
    53941 gatgccaacg actatgtcgg taaaggcatg gcgggcggct taatcgccat tcgtcctccg
    54001 gttggttccg ccttccgcag ccatgaagca agtattatcg gcaacacctg cctgtatggc
    54061 gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac
    54121 tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggc
    54181 ggtattgtct gtattctggg taaaaccggc gttaacttcg gtgcgggcat gaccggcggc
    54241 ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc
    54301 gaggtcttaa gcgttgacga tctggcgatc catgaagagc atctgcgcgg tcttatcacc
    54361 gagcatgttc agcatactgg ctctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc
    54421 ttcgccacta aatttgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt
    54481 catcgtagtc gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg
    54541 agtcagaatg tttatcaatt tatcgacctg cagcgcgttg atccgccaaa gaagccgctg
    54601 aagatccgca aaattgagtt tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa
    54661 gcgcaggctg accgctgcct gtcgtgcggc aacccatact gcgagtggaa atgtccggta
    54721 cacaactaca tcccgaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggcg
    54781 gaactgtcgc accagaccaa caccctgccg gaagtttgcg gacgagtctg cccgcaagac
    54841 cgtctgtgcg aaggttcctg cactctgaac gatgagtttg gcgcggtgac catcggcaac
    54901 attgagcgtt atatcaatga taaagcgttc gaaatgggct ggcgtccgga tatgtccggc
    54961 gtgaagcaaa ccggtaaaaa agtggcgatt atcggcgcag gcccggcagg tctggcgtgt
    55021 gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt
    55081 ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt
    55141 cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac tcaataccga agtgggccgc
    55201 gacgtgcagc tggacgatct gctgagtgat tacgatgccg tcttccttgg cgtcgggact
    55261 tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg atggcgtgta cgcagcgctg
    55321 ccgttcctta tcgccaacac caaacagtta atgggctttg gtgaaacccg cgacgaaccg
    55381 ttcgtcagca tggaaggcaa acgcgtggtg gtccttggcg gtggcgacac tgcgatggac
    55441 tgcgtgcgta cgtccgtgcg ccagggagcg aagcacgtta cctgtgccta tcgtcgtgat
    55501 gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag
    55561 ttcaaattca acgtccagcc actggggatt gaagtgaacg gtaacggtaa agtcagcggc
    55621 gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag
    55681 atcgttgcag gttccgaaca tatcgttccg gcagatgcag tgatcatggc gtttggtttc
    55741 cgtccacaca acatggaatg gctggcaaaa cacagcgtcg agctggattc acaaggccgc
    55801 atcatcgccc cggaaggcag cgacaacgcc ttccagacca gcaacccgaa aatctttgct
    55861 ggcggcgata tcgtccgtgg ttccgatctg gtggtaaccg ctattgccga aggtcgtaag
    55921 gcggcagacg gtattatgaa ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc
    55981 agaaaagagt cggcgctgaa attctccttt ctttctggaa agcgcctcgc ataatctgaa
    56041 gaaaaccaat gtaatacgct catctggatt gttaccggta gatgcccggc gtatagctga
    56101 ttccattatt caacgcagcg ccatataaag ggacagataa tggaatcgct ctcagagaga
    56161 acctcaacag gctaccagca aatccacgac ggtattattc atctggtcga tagcgcccgg
    56221 acggaaacgg tacgtagcgt taacgcgctg atgaccgcga catactggga aattggccga
    56281 cgaattgtcg aatttgaaca aggtggcgag gccagggctg cgtatggtgc gcagctaatc
    56341 aagcgactat caaaggattt aagtctaagg tataagcgtg ggttctctgc aaaaaactta
    56401 cgccaaatga ggctttttta cctctttttt caacatgttg aaattcgcca gacagtgtct
    56461 ggcgaattaa caccattggg aattccgcag acaccatctg cagaattccc atcggcaaag
    56521 atttggcaga cgctgtctgc caaatctttc cccctgccct ggtccaccta cgtccgttta
    56581 ctttccgtta aaaacgctga cgcccgcagc ttttatgaaa aagagacgct ccgctgtggc
    56641 tggtctgttc gccagctaga acggcaaatt gcgacccaat tttatgagcg aacactactg
    56701 tcacatgaca aatcagccat gctgcaacaa cacgctccta ccgagacgca tattcttccg
    56761 caacaggcga tacgcgatcc ctttgtgctc gaatttctgg aattgaaaga tgaatattca
    56821 gaatctgatt tcgaggaggc gctgatcaac cacctgatgg atttcatgct ggaacttggg
    56881 gatgattttg cctttgttgg tcggcagcga aggttacgca ttgatgacaa ctggtttcgt
    56941 gtcgatctgc tgtttttcca ccgccgttta cgctgcctac taatcgtcga tctaaaagtg
    57001 ggcaaattca gctatagcga tgccggacag atgaatatgt atctcaacta cgccaaagag
    57061 cactggacgc taccggatga aaatccgccc atcggtctgg ttctctgtgc agagaaagga
    57121 gccggagaag cacattatgc tctggcaggt ttgcctaaca ccgttctggc aagcgaatat
    57181 aagatgcaac tacctgatga gaaacgactc gcagatgaac tcgttcgaac acaggcggtg
    57241 ctagaggaag gctatagacg ccgttaattt cagcggtttc gaattctgca gacagtgtct
    57301 gcagaatctt taaaacgaac taagttttaa gccattaaat cagccttaac cttcaccaca
    57361 acctttttaa tctctccagg ctcgccgaca acgcatcctg gtttatgcgg ttcacctggc
    57421 ataaacacgg cgaacattcc cggttttaag atgatggctt gctcgttatc aatggtgctg
    57481 caaagctgat aatcatcctc atggtggaac tcttcacact ggcgcgcagt gcctgccatg
    57541 ccaaacagaa tccgttcctc accgtttaat aacagttgga tatcaatgta ttgctcgtgc
    57601 aattccgctt ttttctcgac gggcgattga gtgttaaacg tcatgacatt cataaagata
    57661 ttgtcgccct gtaattcgta acgacccggc gctttttctt gcggtctggc agctaatgcc
    57721 agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag acggtaatga ctgtacttca
    57781 cccatcatca taatttttct ccctgggcca acagcgcagc cccaagtaaa cctgcatcgt
    57841 ggcggtagtg cgccgccagt aaatcaacat gaaatgccgc tggctcctgc gccagatacg
    57901 tttccaccag cgccagatac ccttctgcca gcccaacgct gccaccgacc accacgcact
    57961 ggcaatcagt tgtggcttta atatcagcga tcagccttgc aagcgtacgt gcggagcggt
    58021 gaatcagctg ctgcgcctgc tcgtcgccct gcccggcgcg tgtgaaaata gttctcgcat
    58081 ccgcgcccgc cagttcaccc tgcgctgccg ccgcaatgcc gcgaccagaa gcaatcgcct
    58141 ccacgcaacc tgtgcgtcca cagccacaga ccgggccgtg tggatcggca agcgtatgcc
    58201 cgatgtgccc cgccagaccg ccagggccgg taagcagttt gccgccgctc actacaccgc
    58261 cgccgacgcc agtggaaacg gtgataaaga ccatatcggt tatatcgcct tccagcgcct
    58321 gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc aatggtcggc aaattggtaa
    58381 gttgttccag cgttttgact aacggaaagt gtagcaatcc accgagatta tgcggattca
    58441 gcgccagcaa actgccgtcg cgaataattc cggttgaagc gatggcaacc cgctgcgcat
    58501 gagcttgcaa cggagagact aatgcggata aggcatcacg caaggcttct ggtgtctggc
    58561 tggctggcgt aggaagttca cgacgatcgc ggatctgccc gtcagcgcca atcagcgcgg
    58621 cggcaagttt agtaccgccg atatcaatcg ccagcgtggt catagcaccg cctttttcat
    58681 cgctgtgttg taccactgac aaatgtgttc aagacgcgta atggcagaac cgaccgtcac
    58741 cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca ggcgtgttgt aacgcccttc
    58801 ggcaatcacc ctacatccgg cgtcgctcaa cgttttcacc agcgccagat ccggctcttc
    58861 tggcgtttca ggcgtggtat agccagaaag cgtagtgcca ataatttcgg ctcccagctt
    58921 ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc atcgccagta aaccgtgatg
    58981 gtgaatacgt gccagcagcg tttcaacagg caccggacgc gggcggtcgg tgccgtcaat
    59041 ggcgataatg tccgcgcccg cctgcgccag cgcatcaaca tcttcaatat aggccgtgat
    59101 gcgtaccgga gaatcctcca gatcgcgttt cacaattcca ataatcggca cgctcaccac
    59161 cgcacgcgtg gcttgcagat ttgccacacc ttcaatgcga atggcaaccg cgcccgcctg
    59221 ttctgccgct aatgccatgg cggcgacgat ttcgggttta tcgagcgggc tgtccggaac
    59281 cggctggcag gagacaatca ggccaccgtt agcagcgatt ttttgatcca gttgtgcaag
    59341 taacgacata catcttccct tagcgaaagg cccggtacat agaccgggca acaggattaa
    59401 cttttggttt tgactaaatc gtttttggcg ctgccaaatg gcacggcacc gctaaatggt
    59461 ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc gcagccaacg ctgaacgcgg
    59521 gaaggcatat ccagaccaat cagcaggatc accacaaatg tcagactgaa cgatagcgat
    59581 gccagcgcag tacccagatc cagacgttga gcgatcaacg cgccgatgat tggggccagt
    59641 gcgccgccca atgcgccaac gttgtaggta aagcccaggc ccgctgcacg ctggtcggta
    59701 tcgaaataac cgccaatcag tttcggtaag atcccggcga tcccttgtcc aagcatttgc
    59761 tggaagaaca gtaacagacc gagcacccag acgtttgcgc cgccaatcgc aaataccgga
    59821 ataatcaaca gctgcgaggc caacaggcta caaacgtacg ctttgcgggt tcccagccag
    59881 tcaccgagga agccgccgac acagcacccc accgcagccc caaagccgct aaagaacagc
    59941 acattggcga cagtatgcgg gttataagcc agatcggttt tcagatacgt tggcagcagc
    60001 gcctgaatcg gccatgagta gaggaaagca aacaacacga ccaccatcag cattacgccc
    60061 gttggccagc gtttacccgt actttgcacc ataaagctga taaagattgc ggcgcataac
    60121 agcccaagaa cagcgacgat cgcggcattt tgcaggttac cggcgaagca aaaccacagc
    60181 gcagtagccg ccgccagtgt cattacgata ttggcaatgc gatgttcacc acggtagaga
    60241 atatctacca tcgtgcgtac tggtgcttta cctgcatgtt tctctttcca gtcttccgct
    60301 tccgggatgt ttttacgcag ccagagagca aagatgatag gcaaaatgcc gataaagaac
    60361 agcgcacgcc agccccagac cggaaccacc aggctataga cctgagcggc aacgacggcc
    60421 cccacagaga agcctgaaat cagaaaacca ctggctttgt tacgcagatg ttttggccag
    60481 ctttcaatga cataggtggc gctggaaccg tattcacccg ccatccccat gccgatgacc
    60541 agacgcgcga taaacatggt gatgtagcct ggcgcaaagc cgcaggccag cgtcccggcc
    60601 gagaagagaa cgatgctggt gaccattgcc agacgacgcc cgtagcggtc acccatagcg
    60661 ccgagcatca ggccgccgaa ccagcgagag ataaaggctg cggagatcag acttgccgcc
    60721 tgcaccgtcg tcagcccgaa ttcaccttgt acttcggtga gtaccagggc gattaaaacg
    60781 aaatcaaaac cgtcaagcag atatcccaac caggcagcgg aaaatgcccg ccattgtgca
    60841 cgattgagat ggcgatacca cgggatattc tgggttgtag tactcattgt gagtctcccg
    60901 cggtgggcga tgcccacacg ctttggtatg aaaattgtag ggtacagatg cgtttatttc
    60961 ccctcacccg gtaggggcga gcgaggggaa aacaactcac ccgcgctctt gcatcaactg
    61021 ctgggccagc gccttcagtt ctggcagata tttttcatct accggtccaa acggtttgcg
    61081 gcacagcggc acagaaacga catccatata atggaggaca gttttcaggc cgcggaatac
    61141 gcccgttttg atcagtaaat caatgacttt attgcattca gtttgcagtt tctgcgcggt
    61201 ctggatatcg ccttctttca gcgccttaac gatcccctga tagcgccagc ccatgatgtt
    61261 gtaggtacta ccgataccac catcagcgcc cgccagcaga ccagaggcga agatttcgtc
    61321 gtaaccgtta tagagcacaa gatcaggatg ttcacgacgg atctgctcca tctgatagag
    61381 atcgccagaa gtctgtttca gcgcaccgac gccaggcaac gtaaccaggg tgttgatctg
    61441 atccagggtc agttttaccc cactcagggc tggaatgttg tacaccacca tcggcaaacc
    61501 atccgctgaa tcaataattg cccgatagtg atcgcagtgt tcttcaaagc tgaaaggata
    61561 gtagaacggc gtgacggcgg agacggcatc gaagccataa cgtttagccg atgccgcaag
    61621 ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg atgagtttaa tcttaccttt
    61681 cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg gaaaggcttt gtacaaaggc
    61741 ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc tgctgaatat tgaactgaac
    61801 caggcgacgc agactcgctt tatccagtgc ttgttgttga tcaaaaggag tcaggagtgc
    61861 agccattacg ccacgtaaat tcgttgccat aaatacctct gaagtgatgc ttgtctgata
    61921 aacgatatac ctttatacct gttataccag atcaattaag caacacccca tacagaaagc
    61981 ttataatgcg atctgcttca ctaaagtggc attatttctt tttgttggtg gtctgaccga
    62041 aagcgtgcca ggtagcagag acgctgttga gatgcgattg caacgcacga tcggcttcgt
    62101 caggatcatg acggcggatc gcatcaacga tcgcaatatg ctgttgataa ctaacgttgt
    62161 tatgttcgtg cagtgcctga tcggtaaccg ttgggcgtgc ggcaataagc cagtcgagca
    62221 gggcaacgtg gatcgccatg aagattgggt taccggggat ctccgccagc acgcggtgga
    62281 aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag cgactgactg ttaatttcca
    62341 gtgcttttgc cagcaaatcg atttgctcat cggtggcatg ttcagccgca tagcgcacca
    62401 ggctggattc aaagaacaga cgtaattgtt cgaaatgggc aatcccacca ggatgagaaa
    62461 ggaaatcttt cgccattccg gaaagctcgc cgatgatggt gtccgcagaa ggacgtgaga
    62521 cgcgggcacg ttcgccgttg ttgatttgca ccagaccttt gcgttttaac gctgccagcg
    62581 cttcgcgcac agaaggacgc ccgacgttaa agaacgccat cagttcgcgt tcagacggta
    62641 attgttcacc ttcgccaaat tcacgacggc ggatcatctg ttccagctct tcttccacca
    62701 tttcggagag ttttttacgc gccagcgggc ggctacgcaa gttgcgacca attgcaggtg
    62761 aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc cattctgtaa ggtcagtgtg
    62821 attaacatca tcagtgacat cctatcacag gattgaaagt aggggaaaat ggcaggtttt
    62881 tctctttgtg cctcatcatt accataatta acggaataat taactattgc gaaaaattaa
    62941 tgtaacgcag at
//
LOCUS       DAZFVO020000007        56604 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFVO020000007 DAZFVO020000000
VERSION     DAZFVO020000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 56604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.007"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..820)
                     /gene="rfbA"
                     /locus_tag="ACHKNB_RS01915"
                     /old_locus_tag="pgaptmp_000383"
     CDS             complement(<1..820)
                     /gene="rfbA"
                     /locus_tag="ACHKNB_RS01915"
                     /old_locus_tag="pgaptmp_000383"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4193962.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQFSFIRLLGDGKHLGVSISYAVQPRPDGLAQAFTIGKDFIGADSV
                     CLVLGDNIFFGQGFTPKLKTACERKLGATIFGYQVRDPGRFGVVEFDSDYRVLSIEEK
                     PLKPKSNWAVTGLYFYDNDVVEIAKSITPSQRGELEITSINEVYLKNNKLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETVQKRQGMMVACPEEIAWRNGWLNNSDLKSLATNM"
     gene            complement(817..1893)
                     /gene="rfbB"
                     /locus_tag="ACHKNB_RS01920"
                     /old_locus_tag="pgaptmp_000384"
     CDS             complement(817..1893)
                     /gene="rfbB"
                     /locus_tag="ACHKNB_RS01920"
                     /old_locus_tag="pgaptmp_000384"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4193963.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(2266..3159)
                     /gene="galF"
                     /locus_tag="ACHKNB_RS01925"
                     /old_locus_tag="pgaptmp_000385"
     CDS             complement(2266..3159)
                     /gene="galF"
                     /locus_tag="ACHKNB_RS01925"
                     /old_locus_tag="pgaptmp_000385"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HGU4193964.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGANFRKGIEKLLSE"
     gene            complement(3334..4728)
                     /gene="wcaM"
                     /locus_tag="ACHKNB_RS01930"
                     /old_locus_tag="pgaptmp_000386"
     CDS             complement(3334..4728)
                     /gene="wcaM"
                     /locus_tag="ACHKNB_RS01930"
                     /old_locus_tag="pgaptmp_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HGU4193965.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPDQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4739..5959)
                     /gene="wcaL"
                     /locus_tag="ACHKNB_RS01935"
                     /old_locus_tag="pgaptmp_000387"
     CDS             complement(4739..5959)
                     /gene="wcaL"
                     /locus_tag="ACHKNB_RS01935"
                     /old_locus_tag="pgaptmp_000387"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HGU4193966.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5956..7236)
                     /gene="wcaK"
                     /locus_tag="ACHKNB_RS01940"
                     /old_locus_tag="pgaptmp_000388"
     CDS             complement(5956..7236)
                     /gene="wcaK"
                     /locus_tag="ACHKNB_RS01940"
                     /old_locus_tag="pgaptmp_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HGU4193967.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(7415..8893)
                     /gene="wzxC"
                     /locus_tag="ACHKNB_RS01945"
                     /old_locus_tag="pgaptmp_000389"
     CDS             complement(7415..8893)
                     /gene="wzxC"
                     /locus_tag="ACHKNB_RS01945"
                     /old_locus_tag="pgaptmp_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HGU4193968.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWISIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8895..10289)
                     /gene="wcaJ"
                     /locus_tag="ACHKNB_RS01950"
                     /old_locus_tag="pgaptmp_000390"
     CDS             complement(8895..10289)
                     /gene="wcaJ"
                     /locus_tag="ACHKNB_RS01950"
                     /old_locus_tag="pgaptmp_000390"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HGU4193969.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(10344..11714)
                     /gene="cpsG"
                     /locus_tag="ACHKNB_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /gene_synonym="manB"
     CDS             complement(10344..11714)
                     /gene="cpsG"
                     /locus_tag="ACHKNB_RS01955"
                     /old_locus_tag="pgaptmp_000391"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HGU4193970.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     SYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11907..13343)
                     /gene="cpsB"
                     /locus_tag="ACHKNB_RS01960"
                     /old_locus_tag="pgaptmp_000392"
     CDS             complement(11907..13343)
                     /gene="cpsB"
                     /locus_tag="ACHKNB_RS01960"
                     /old_locus_tag="pgaptmp_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HGU4193971.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(13346..14569)
                     /gene="wcaI"
                     /locus_tag="ACHKNB_RS01965"
                     /old_locus_tag="pgaptmp_000393"
     CDS             complement(13346..14569)
                     /gene="wcaI"
                     /locus_tag="ACHKNB_RS01965"
                     /old_locus_tag="pgaptmp_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HGU4193972.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14566..15045)
                     /gene="gmm"
                     /locus_tag="ACHKNB_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(14566..15045)
                     /gene="gmm"
                     /locus_tag="ACHKNB_RS01970"
                     /old_locus_tag="pgaptmp_000394"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HGU4193973.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(15045..16013)
                     /gene="fcl"
                     /locus_tag="ACHKNB_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(15045..16013)
                     /gene="fcl"
                     /locus_tag="ACHKNB_RS01975"
                     /old_locus_tag="pgaptmp_000395"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HGU4193974.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(16016..17137)
                     /gene="gmd"
                     /locus_tag="ACHKNB_RS01980"
                     /old_locus_tag="pgaptmp_000396"
     CDS             complement(16016..17137)
                     /gene="gmd"
                     /locus_tag="ACHKNB_RS01980"
                     /old_locus_tag="pgaptmp_000396"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HGU4193975.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(17163..17711)
                     /gene="wcaF"
                     /locus_tag="ACHKNB_RS01985"
                     /old_locus_tag="pgaptmp_000397"
     CDS             complement(17163..17711)
                     /gene="wcaF"
                     /locus_tag="ACHKNB_RS01985"
                     /old_locus_tag="pgaptmp_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HGU4193976.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17727..18473)
                     /gene="wcaE"
                     /locus_tag="ACHKNB_RS01990"
                     /old_locus_tag="pgaptmp_000398"
     CDS             complement(17727..18473)
                     /gene="wcaE"
                     /locus_tag="ACHKNB_RS01990"
                     /old_locus_tag="pgaptmp_000398"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HGU4193977.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(18484..19701)
                     /gene="wcaD"
                     /locus_tag="ACHKNB_RS01995"
                     /old_locus_tag="pgaptmp_000399"
     CDS             complement(18484..19701)
                     /gene="wcaD"
                     /locus_tag="ACHKNB_RS01995"
                     /old_locus_tag="pgaptmp_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HGU4193978.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19676..20893)
                     /gene="wcaC"
                     /locus_tag="ACHKNB_RS02000"
                     /old_locus_tag="pgaptmp_000400"
     CDS             complement(19676..20893)
                     /gene="wcaC"
                     /locus_tag="ACHKNB_RS02000"
                     /old_locus_tag="pgaptmp_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HGU4193979.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20890..21378)
                     /gene="wcaB"
                     /locus_tag="ACHKNB_RS02005"
                     /old_locus_tag="pgaptmp_000401"
     CDS             complement(20890..21378)
                     /gene="wcaB"
                     /locus_tag="ACHKNB_RS02005"
                     /old_locus_tag="pgaptmp_000401"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HGU4193980.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(21381..22220)
                     /gene="wcaA"
                     /locus_tag="ACHKNB_RS02010"
                     /old_locus_tag="pgaptmp_000402"
     CDS             complement(21381..22220)
                     /gene="wcaA"
                     /locus_tag="ACHKNB_RS02010"
                     /old_locus_tag="pgaptmp_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HGU4193981.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(22398..24560)
                     /gene="wzc"
                     /locus_tag="ACHKNB_RS02015"
                     /old_locus_tag="pgaptmp_000403"
     CDS             complement(22398..24560)
                     /gene="wzc"
                     /locus_tag="ACHKNB_RS02015"
                     /old_locus_tag="pgaptmp_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HGU4193982.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(24563..25006)
                     /gene="wzb"
                     /locus_tag="ACHKNB_RS02020"
                     /old_locus_tag="pgaptmp_000404"
     CDS             complement(24563..25006)
                     /gene="wzb"
                     /locus_tag="ACHKNB_RS02020"
                     /old_locus_tag="pgaptmp_000404"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HGU4193983.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(25012..26151)
                     /gene="wza"
                     /locus_tag="ACHKNB_RS02025"
                     /old_locus_tag="pgaptmp_000405"
     CDS             complement(25012..26151)
                     /gene="wza"
                     /locus_tag="ACHKNB_RS02025"
                     /old_locus_tag="pgaptmp_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HGU4193984.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            26460..26609
                     /locus_tag="ACHKNB_RS02030"
                     /old_locus_tag="pgaptmp_000406"
     CDS             26460..26609
                     /locus_tag="ACHKNB_RS02030"
                     /old_locus_tag="pgaptmp_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4193985.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            26810..28393
                     /gene="yegH"
                     /locus_tag="ACHKNB_RS02035"
                     /old_locus_tag="pgaptmp_000407"
     CDS             26810..28393
                     /gene="yegH"
                     /locus_tag="ACHKNB_RS02035"
                     /old_locus_tag="pgaptmp_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HGU4193986.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(28667..30520)
                     /gene="asmA"
                     /locus_tag="ACHKNB_RS02040"
                     /old_locus_tag="pgaptmp_000408"
     CDS             complement(28667..30520)
                     /gene="asmA"
                     /locus_tag="ACHKNB_RS02040"
                     /old_locus_tag="pgaptmp_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HGU4193987.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILNAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(30542..31123)
                     /gene="dcd"
                     /locus_tag="ACHKNB_RS02045"
                     /old_locus_tag="pgaptmp_000409"
     CDS             complement(30542..31123)
                     /gene="dcd"
                     /locus_tag="ACHKNB_RS02045"
                     /old_locus_tag="pgaptmp_000409"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4193988.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(31215..31856)
                     /gene="udk"
                     /locus_tag="ACHKNB_RS02050"
                     /old_locus_tag="pgaptmp_000410"
     CDS             complement(31215..31856)
                     /gene="udk"
                     /locus_tag="ACHKNB_RS02050"
                     /old_locus_tag="pgaptmp_000410"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGU4193989.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            32174..35491
                     /gene="dgcE"
                     /locus_tag="ACHKNB_RS02055"
                     /old_locus_tag="pgaptmp_000411"
     CDS             32174..35491
                     /gene="dgcE"
                     /locus_tag="ACHKNB_RS02055"
                     /old_locus_tag="pgaptmp_000411"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4193990.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGGLVVLLTAGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERTA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(35530..36387)
                     /gene="alkA"
                     /locus_tag="ACHKNB_RS02060"
                     /old_locus_tag="pgaptmp_000412"
     CDS             complement(35530..36387)
                     /gene="alkA"
                     /locus_tag="ACHKNB_RS02060"
                     /old_locus_tag="pgaptmp_000412"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HGU4193991.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            36521..37873
                     /gene="yegD"
                     /locus_tag="ACHKNB_RS02065"
                     /old_locus_tag="pgaptmp_000413"
     CDS             36521..37873
                     /gene="yegD"
                     /locus_tag="ACHKNB_RS02065"
                     /old_locus_tag="pgaptmp_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4193992.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(37886..39826)
                     /gene="yegI"
                     /locus_tag="ACHKNB_RS02070"
                     /old_locus_tag="pgaptmp_000414"
     CDS             complement(37886..39826)
                     /gene="yegI"
                     /locus_tag="ACHKNB_RS02070"
                     /old_locus_tag="pgaptmp_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HGU4193993.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEQLRQAAEKLAQAQADLSRC"
     gene            complement(39823..40584)
                     /gene="pphC"
                     /locus_tag="ACHKNB_RS02075"
                     /old_locus_tag="pgaptmp_000415"
     CDS             complement(39823..40584)
                     /gene="pphC"
                     /locus_tag="ACHKNB_RS02075"
                     /old_locus_tag="pgaptmp_000415"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HGU4193994.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(40581..41240)
                     /gene="yegL"
                     /locus_tag="ACHKNB_RS02080"
                     /old_locus_tag="pgaptmp_000416"
     CDS             complement(40581..41240)
                     /gene="yegL"
                     /locus_tag="ACHKNB_RS02080"
                     /old_locus_tag="pgaptmp_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4193995.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLHSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(41461..41520)
                     /gene="ibsA"
                     /locus_tag="ACHKNB_RS02085"
                     /old_locus_tag="pgaptmp_000417"
     CDS             complement(41461..41520)
                     /gene="ibsA"
                     /locus_tag="ACHKNB_RS02085"
                     /old_locus_tag="pgaptmp_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HGU4193996.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(41793..41849)
                     /locus_tag="ACHKNB_RS02090"
                     /old_locus_tag="pgaptmp_000418"
     CDS             complement(41793..41849)
                     /locus_tag="ACHKNB_RS02090"
                     /old_locus_tag="pgaptmp_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4193997.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            42128..43375
                     /gene="mdtA"
                     /locus_tag="ACHKNB_RS02095"
                     /old_locus_tag="pgaptmp_000419"
     CDS             42128..43375
                     /gene="mdtA"
                     /locus_tag="ACHKNB_RS02095"
                     /old_locus_tag="pgaptmp_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HGU4193998.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKVGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            43375..46497
                     /gene="mdtB"
                     /locus_tag="ACHKNB_RS02100"
                     /old_locus_tag="pgaptmp_000420"
     CDS             43375..46497
                     /gene="mdtB"
                     /locus_tag="ACHKNB_RS02100"
                     /old_locus_tag="pgaptmp_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HGU4193999.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSAHLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGVSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            46498..49575
                     /gene="mdtC"
                     /locus_tag="ACHKNB_RS02105"
                     /old_locus_tag="pgaptmp_000421"
     CDS             46498..49575
                     /gene="mdtC"
                     /locus_tag="ACHKNB_RS02105"
                     /old_locus_tag="pgaptmp_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HGU4194000.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFTST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            49576..50991
                     /gene="mdtD"
                     /locus_tag="ACHKNB_RS02110"
                     /old_locus_tag="pgaptmp_000422"
     CDS             49576..50991
                     /gene="mdtD"
                     /locus_tag="ACHKNB_RS02110"
                     /old_locus_tag="pgaptmp_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194001.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNCFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            50988..52391
                     /gene="baeS"
                     /locus_tag="ACHKNB_RS02115"
                     /old_locus_tag="pgaptmp_000423"
     CDS             50988..52391
                     /gene="baeS"
                     /locus_tag="ACHKNB_RS02115"
                     /old_locus_tag="pgaptmp_000423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HGU4194002.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPVPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            52388..53110
                     /gene="baeR"
                     /locus_tag="ACHKNB_RS02120"
                     /old_locus_tag="pgaptmp_000424"
     CDS             52388..53110
                     /gene="baeR"
                     /locus_tag="ACHKNB_RS02120"
                     /old_locus_tag="pgaptmp_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HGU4194003.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            53301..53633
                     /gene="yegP"
                     /locus_tag="ACHKNB_RS02125"
                     /old_locus_tag="pgaptmp_000425"
     CDS             53301..53633
                     /gene="yegP"
                     /locus_tag="ACHKNB_RS02125"
                     /old_locus_tag="pgaptmp_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HGU4194004.1"
                     /translation="MAGWFELIKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            53842..54138
                     /locus_tag="ACHKNB_RS02130"
                     /old_locus_tag="pgaptmp_000426"
     CDS             53842..54138
                     /locus_tag="ACHKNB_RS02130"
                     /old_locus_tag="pgaptmp_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4194005.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            54140..54436
                     /locus_tag="ACHKNB_RS02135"
                     /old_locus_tag="pgaptmp_000427"
     CDS             54140..54436
                     /locus_tag="ACHKNB_RS02135"
                     /old_locus_tag="pgaptmp_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4194006.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            54539..55900
                     /gene="yegQ"
                     /locus_tag="ACHKNB_RS02140"
                     /old_locus_tag="pgaptmp_000428"
     CDS             54539..55900
                     /gene="yegQ"
                     /locus_tag="ACHKNB_RS02140"
                     /old_locus_tag="pgaptmp_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HGU4194007.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(56230..56547)
                     /gene="yegR"
                     /locus_tag="ACHKNB_RS02145"
                     /old_locus_tag="pgaptmp_000429"
     CDS             complement(56230..56547)
                     /gene="yegR"
                     /locus_tag="ACHKNB_RS02145"
                     /old_locus_tag="pgaptmp_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HGU4194008.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT        13279 a        15127 c        14386 g        13812 t
ORIGIN      
        1 tcatattagt tgctaatgat ttcaaatcag aattatttaa ccaaccattt cgccaagcaa
       61 tttcttcagg acacgctacc atcataccct gtcttttttg tacagtttca acgaaacttc
      121 ccgcctcaat taggctatcg tgcgtgcctg tatctaacca tgcgaaacca cgcccaagca
      181 gttcgaccgt caacttgttg ttttttagat aaacttcatt tatcgaagtt atttctagct
      241 cacctcgttg agaaggagta attgatttgg caatttcaac aacatcatta tcataaaaat
      301 aaagtccagt tacagcccag tttgattttg gttttaacgg tttttcttca atacttaata
      361 ccctataatc tgagtcaaac tctacaactc caaaccgtcc tggatctctt acctgatagc
      421 caaaaattgt ggctcctaat ttcctttcac atgcagtttt taattttggt gtaaaccctt
      481 gaccaaaaaa aatattatca cctaatacaa ggcaaactga atcagcacct ataaaatctt
      541 ttccaattgt gaaagcttgc gctaaaccat ctggccttgg ttgcactgca taactaatgc
      601 tgacacctag atgttttccg tcaccaagca accgtataaa actaaattgg tcttcgggcg
      661 ttgtaataat aagtatatct ctgatccctg ccaacatcag aacagacaat ggataatata
      721 tcattggctt gtcataaaca ggcaataatt gctttgaaat ccccattgta attggataaa
      781 gccttgttcc agaaccacca gctaaaataa ttcctttcat ctaattaccc ccaaacgttc
      841 acgttgataa gagccatctt gaactcgccg gcaccaatca ttattattca aataccagtt
      901 aattgtttta cgaatcccgc tctcaaacgt ttcctgtggt ttccatccca attcgcggct
      961 aatcttctca gcatcaatcg cataacggcg atcgtgtccc ggacgatcgg caacataagt
     1021 gatttgctca cgataagatt tctctttcgg tacaatctca tccaacaaat cacaaatagt
     1081 gagcactaca tcgatgtttt tcttttcgtt gtgtccacca atgttataag tttcacccgc
     1141 tttaccttcg gttacgacgg tatataacgc acgtgcatga tcttcaacat acagccagtc
     1201 gcgaatttga tcccctttgc cataaatagg taatgcctta ccttccagag cattaagaat
     1261 tactagtgga atcaattttt ccggaaagtg ataaggaccg tagttattcg aacaattagt
     1321 cacaatggtc ggtaaaccat aggtacgttt ccacgcacgg actaaatgat cgctggatgc
     1381 tttggatgcg gaataagggc tgcttggcgc gtaagctgtt gtctcagtaa ataagggtaa
     1441 ttcttctgta ttatttacct cgtcaggatg aggcaaatca ccatatactt cgtcagtaga
     1501 aatatgatga aaacggaagc tatttttctt gtcgctatca agagcagacc agtaattgcg
     1561 agcggcttcc aaaaggacat aagtaccaac aatattggtt tcaataaatg ccgcagggcc
     1621 tgtaattgaa cgatccacat ggctttcagc agccaggtgc atcactgcat ccggctgatg
     1681 ctgagcaaaa atccgtgcca ttgcagcagc atcgcaaata tccgcatgtt caaaaatata
     1741 gcgttcagaa tcagaaacat cagcaagtga ttccaggttt ccggcgtacg ttaatttatc
     1801 gacattaaca acactatcct gcgtattatt tataatgtga cgaactacag cagaaccaat
     1861 aaatcctgcg ccaccagtaa caagtatttt cacttaattt attccatatt acatcagagc
     1921 atgctgtgaa ataagcggct ctttgtttga ttaatagagg tattaatgca cgctaccgcc
     1981 cctggcttta cagctaccag agcactgcat gcatgtctac gatgtgacga gcgttaccca
     2041 ctcgcgctaa acccgaaaaa ttcaaacgct aattgtctta ccaatccgct ctggaaacaa
     2101 ggaaaatcct ggaaaacttt gactaaaatc ctattgctaa ctcgttgtta ttctgattgt
     2161 ttatataaaa caacggcagg aatattcgca gcaaattact ttcgccacga atcttcactg
     2221 ccgttataat tttcttatca accgttacat ccggtcagat tttcattatt cgcttaacag
     2281 cttctcaata cctttacgga aattcgcccc ttctttcagg ttgcgtagtc catacttcac
     2341 aaatgcctgc atataaccca tttttttacc gcagtcgtag ctgtctccag tcatcagcat
     2401 ggcatcaacg gactgttttt tcgccagttc agcgatggca tcagtcagct gaatacgtcc
     2461 ccatgcacca ggctgagtgc gttcaagttc cggccaaata tcggcagaaa gcacataacg
     2521 gccaacggcc atgatgtctg agtccagcgt ctgcggctga tccggttttt cgataaattc
     2581 gacgatgcgg ctgactttac cttcgcggtc cagcggctct ttggtctgaa tgacggagta
     2641 ttcagagagg tcacccggca tacgttttgc cagcacctgg ctgcgtcctg tttcattgaa
     2701 gcgcgcaatc atggcagcaa ggttgtagcg cagcgggtcg gcgctggcgt cgtcgatcac
     2761 aacgtctggc agcaccacga caaatgggtt gtcaccaatg gcgggtcgtg cacataaaat
     2821 ggagtggccc aaacctaaag gttcgccctg acgcacgttc ataatggtca cacccggcgg
     2881 acagatggac tgcacttccg ccagcagctg acgcttcacg cgctgttcaa ggagagattc
     2941 taattcataa gaggtgtcga agtggttttc gaccgcgttc ttggacgcgt gagttaccag
     3001 gaggatttct ttgatccctg cagccacaat ctcgtcaaca atgtactgaa tcattggctt
     3061 gtcgacgatc ggtagcatct ctttgggaat cgccttagtg gcaggcaaca tatgcatccc
     3121 aagacccgct acaggaataa ctgcttttaa attcgtcatt atttcatcca cctgtaaaat
     3181 ggttgctgaa ttatagcttg ttcgattttt ttcgccagca tcaattaccc tgaattgatt
     3241 actgaattac ttgtgatgtt acgccgcttc gttgtggatt gcagtagcat tgttcctaag
     3301 tatggctcca tttttccagg aatggtcgca aatctactcc ctccgttctg gcaatctaaa
     3361 gttaatcttt tccacattaa caatatggtg attaatcctg tcgatatcga cggagctttg
     3421 ccctctttca ttcaccgcat gaacattcgc aagagatagc agtgtttctt ttttcgccat
     3481 aaagacgccg cgaacgtctt tgcgcatgtc aaagttcatg ctcaatgctg gtccaactga
     3541 ggattcctgc atcaccttga tatttcgcat aaaaagatgt tgcggtttgt tgtgtaactc
     3601 cagcgaggca cgcttcatct caatgttagt cagcgccaca aaggagagag cattcccggc
     3661 ggagatttga atgccgcgca atttgtaagc aaggtgggtg ttatccagtt gaatattatt
     3721 cactcggaaa ttttgcggta tcgagagata tttgccttta attaccccat agccgattaa
     3781 catcccggcg ctattaatca tttcaatatt atcaatcacg aaattgtcac aaccgtaaat
     3841 agcaactgtc gcgttatcaa tgcctgcttt cttactgaaa tccggcgtga tattgcgggc
     3901 tttgatatta cgaataacaa aatgtttacc attttcaaca tggatcaact gccgacaatc
     3961 cgatcccgtg atattcgcca cgacaaagtt tttcactgcc tggtcttccg ggtagttgtt
     4021 atcgtaggta cttcccgcaa ggcctatgcc aatgccccag ttgattttgc cattagtaca
     4081 gttgatgcgc tcgatgacat ggtcggagat caaaatatca cggtcgttaa tcgccacgtt
     4141 ccattcaatg gcatcgccct gtaagtcgct gaacttacaa ttggtgatgt tggcaccgat
     4201 tatctggtta tgaaatccct ggcgtaagat ggcgtaatta gcgtggctaa cggtcaggtt
     4261 atcgatggtc aggttacgca tgacccgttt gtttttgccg ccgatataga tctgcgtcac
     4321 cgggccaaag ccgctcatcg tcagcccttt gatggtgcag tcagaaccac gcacatccag
     4381 ggtgatgtta tgcatactgc cgcccttctc ccctgtcacc tggctgccgt cctgtaagac
     4441 aaatcgccct ctgccgttgc ctcgcaggct tccgaggatg tgtaacgttt tgccaggagg
     4501 aatgaagatg ccggtgttga tattgtcaca aacgaatccg gcaggcacga cgaccgtttg
     4561 cccttcgctg aaggcttgtt taaatgaggc gatccagtcg tgtgggttgt agtcgttaat
     4621 gttaacgctc tgttggtcgg gaagtgcgcg agcgaaaggg gtatggagga aggcaagcgc
     4681 cgagcttgcc gtcaggaacg tgcgtcggga gagtttttta aatggcatgc gttctcctct
     4741 ataaagcctg cagcaagctg gcgagttctc gattgatcac ctgctggtta aaatcgtgtt
     4801 cgactttttc gcgcgcacgt ttgacaaccg gagccagttc gtcagtgtcc agttggctaa
     4861 atgccgccag acgttgtgcc agtgcgcgag catcgttctc aggcaccagc cagccggatt
     4921 tatcggcctc caccagttcc ggtattccgc tatgcagagt agaaactact ggaatgccga
     4981 ccgccatcgc ttccattagc gccactggaa tgccttccat atcaccatcc gcgcccgtta
     5041 ccgatggcaa caggaagaca tccgcgtcgt cgagcatcgc tttcacttca tggctcggtt
     5101 taaagcccgg catctctacc acatcttcca gttgatattg ttcgatgagg gtgcgcaggc
     5161 gtcgttccca cgggccaatg ccgagaatgc gatagcgaaa tgccacgccc tgctctttca
     5221 actgacggca ggcttcaatc gccacatgca gacctttttt ctcggttaag cgtgcgacgg
     5281 aaatgatttc cagcggcgtt gcgggtgctt tcaccggacg cgggctaaag cgcgtcatgt
     5341 ccacgcccat acgcgatacg gcgatttttt ccctcggaca gcccattttt tgcagccttc
     5401 cggcccacag atcgcttatc ggtaacatca ggtcgccacg gcgaaacagt tgttgatatt
     5461 cgggagtgta gtggttgaga acgtcccgac tggagatatc aataccgtgg aagatagtgg
     5521 caattttgcc gcgaataaca cccagttcgc gtagttttgc tgcggttacc cccgccgggc
     5581 caaaatgagc gataaagaca tcggcatgaa acggtgttgc gacctggcca caaatggcag
     5641 acaaaatcag gttccgcgac tcggcaccat agcgtttgag gttgagcgcc tgccaggtat
     5701 ttttacgata aatgccgcgt aaggtctggc tggcgcggtg gcgcaatttc gctactttgc
     5761 ccgcaggttc gtcctgtaac caacgggttc tggcggcaag gttgtatttc gtccatgccg
     5821 cgtgggtgtt ttgtgtgtcg cctttttgca gcgcgacaat ctccacctca aatcccatat
     5881 caataaacgc agtgatttgg ttgaggacga aggtttccga cgacagcggg aatttcagta
     5941 aaaagaagcc gaccttcatt tcacctcccc gatgcgctca agcacagact gtaccatctg
     6001 cattcctgtc tgacgctcac gactgacggc ttcgctaagt cgtgcgttca gcgccggaag
     6061 ctggcctaag gtatccgcaa ccatcgcttg caggctgccg tctaataaat gacggatatc
     6121 aattgccatc tctggcagtc ccaactgttg cataatcccg gcggatttat gttcgtagtt
     6181 gatggcaatt gccggagtgg caaaattcat cgagataatg gcagagtgca ggcgcgtacc
     6241 gacggtgagt tcacaggcac cgagaatttt gcccatttcc agatcgttaa gttcatccat
     6301 cactacgtga taacgggcag gatcactgat gtgctggcgc aggttgagcg ccaccatgcg
     6361 gtcgtctttg ttatagctgt cgatacccgt acaggtggag agcgcaataa cctgatagcc
     6421 ttcgtcgaga atgcgattga ccaccccggc aaaggctttt tcatacgctt gttgagtggt
     6481 gccgagacgt ttgtcgaacg gtgccagttc gcgcagggta atggccaccg ttttctgttg
     6541 tgcggcaacg tccagccagt gttgaacggc atagctggcg gtgaaatctt cggtgtggtg
     6601 atcgaccagc cacgcggtat cgacgccatg ttccactttt gcggtggtga tattgctgcg
     6661 tttcatcaga tcaaggctga ccgattcgcg caggatcagc gcgtcgcagt gaccaaaaac
     6721 gtagttcgcc agttggttaa attgctcatc ctggaacggg ccaacactgt gaccaatcat
     6781 aaacagcggc tttttcgcca tgaaagtaca aagtgcatgt tcaaactgcg gcacgccata
     6841 gagatcgaca aaaaacgatc cgccgacctg aataatggcg tcgtaacctg acagcaggcg
     6901 cacgaagtcg gtgaatccct gggcgatggc gatattacgc agcttgccag tgtcggtgac
     6961 gcgtgagagc aatacctggt gttgatagcg gcgacggagg acttttttaa cgcgcccgac
     7021 aacgcccgcc gcgctgttgt gttgtttcat ttgcaggaac agcggatcgc ccattaccgg
     7081 gcggttgagc agccaggaag aactgaccgg atagcggctc atcacgtcca cttcggcttg
     7141 tggattgagg atgttgatgg catcaagtaa gccgcgcagg atggcgctgt cgccacgatt
     7201 gccgcaagtg tggttgccca gaataagtaa tttcattttt tcctcataaa tttgatgcca
     7261 ggtgaggctg cgtttgtttt gttcgttgcc gcttttttcc tgatgcgacg ctgacgcgtc
     7321 ttatcaggcc tacaagaccc gagcctagaa ccgtaggccg gataaggcgt ttacgccgca
     7381 tccggcaacc gttgccggaa ccgaaaacaa caactcaccc cgcccgtaaa agcattttca
     7441 ttttttcgct gcgacaaaac tgacgcttta cttccaccac cagcggatgg cgcgacagca
     7501 caatcatcac cacaaacgcc agcacacccg tggctatttg caccgccagc agcatcccca
     7561 gcgccagttg ccctttcagc acaatgccca gcgcataact gaccaccagc gtcggcagcg
     7621 agagataaaa cggcagccat aaactcagga tgtactggcg ataacttgaa ccgagtactg
     7681 gtttaatcat cacgaaataa ctcagaatgg tgttgataat ctgcaccagc aggaaaccga
     7741 gcgtgacgcc gatcgcgccc gccatctgcc caccaataac aatcgccgga ataaacagaa
     7801 atgttttgaa tacgttgaat ttaaagctga tatcgacccg cgctttcgcc atcagcagcg
     7861 aaccaatcgg gttacccact gagcgcagca gacccacaat acacagcaat tgcagcaccg
     7921 gaataatgct gatccacttc tcaccaaaga ccagcggtac aaaattattc gacaccacca
     7981 tcagcccgag cagcgccgga aagttgataa tccccaccac cgacagcagc ttgtagaagt
     8041 tgacgcgcag cttttcggtg tcgtcctgaa ttttggcaaa cgccggaaac aacacgcggg
     8101 tgatgatggg gttcagcttc atcggcggca caacggccac gttgtacgcc aggttgtatc
     8161 cccctgctac gcccgcgccg agaatacgcg ccagcaccag cgttgaaagg ttggtgttga
     8221 gatagttgat gatgctgtcc gccgtcagcc aggcaccaaa acgtaagttc ggtgccacag
     8281 acgccagcga gaagtgcaga ccggggcaat aaattttgcg gccaaagtag ccaaacagca
     8341 gcgttcttac cgcactattg accagataac cgaggatcgc ggtcatcgcc agcggccaga
     8401 aatgggcgct aaccactgta aaagtgaagc ccgccagcac cgcgctggtt tcgatcatgc
     8461 cgattttgtt gaactccagt tccttttgca tcaacgcgcg gaactgctgc ccgtggggga
     8521 tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc caggtccggg ttattcagca
     8581 cgtcaccaat ggcatcactc aacaaaaaca ccgccacgca caccacaagc cccagcccga
     8641 cgttcagcca gtacagcgtg gtgagttcaa ggtggctgat ttcttttcgc tgaataatcg
     8701 agttagcgat accgaagtca gaaagcgtat ctgccagcgc gatgatcacc agcgacacgg
     8761 taagcaaacc gaactggtgg ttgtcaataa tccgcgccag cacggtcatc tgtaccagcc
     8821 cgaggccgat gatgatcacc gtagcaatcg ccgaccactt cgcgccgctg atggtttttt
     8881 cacgtaagct catatcaata tgccgctttg ttaacgaaac ctttgaatac cgtcaggaaa
     8941 acgattttaa tatcgaacca gacgctccat tcgcggatgt actcaaggtc gaactcgacg
     9001 cgtttttcca ttttctccag cgtgtcggtt tcgccacgcc agccattaat ctgcgcccag
     9061 ccggtaatgc ccggtttcac tttatggcgc agcatgtagc cttcaatgag ctgtcgatac
     9121 tgttcgttat gcgccaccgc gtgcggacgt ggaccgacaa tcgacatccc gccggtcagc
     9181 acattgataa actgcggcag ttcatccagc gaggtgcggc gcagaaagtt ccccactttg
     9241 gtgacgcgcg gatcgttctg cgtcgcctgg gtcaccactt tgtcgttctc catcactttc
     9301 atcgaacgga acttccacac tttgatcggc ttgccatcca tgccatagcg agtctggcgg
     9361 aaaataaccg gccctggtga actgagtttc accgccagcg caatacagca cagcaccggg
     9421 gagatcagca gcaagatcag cgtcgccagc acaatgtctt ccgcacgttt tatcaggcgg
     9481 ttaaccccgg aaagcggcgt atcgtacagc ggcaccaccg gaacgccgtt catctcttcg
     9541 agacgtgaat ggagaatgtt gaaggtaaaa acatcgggga tcagcagcac cgaacaggtg
     9601 gtgtccgcca attgatggac cagttttttc actcgcgcgc cgtcgcacat ttgcatcgcg
     9661 atatagacgt tatgaatctt acccgcttta gcatcctcga ccagctgttg caggttaccc
     9721 gcccagtcgt tagaaacgcc gcccggtttc gggtcgtggt aaacgcccac cacttcaaac
     9781 cctaaccacg gctgattacg gaagctctcc atcagcattt gtccggcggc taaatccccc
     9841 gccaccgcga ccatgcgctt gttatagcca tgattacgca gccagcccgc cccaatgcga
     9901 atacatgaac ggcaaaccac cagtccgata ctggtcagcc cataccacgc cagccagatt
     9961 ttcagttgcg tgtcgaaatc attgttgaac gccaccagtc cggcactgaa aatcacgctt
    10021 aaggtccagt tttgtagcaa tagggcaaat tctgtcgctg cccgaacacc gcgccatgag
    10081 cgataaaaat cggtgatgcc acccagcatc tggaacacca ccagcgtaat cagcgccacc
    10141 aacaggtgca tgtagaggaa tgacagtccg ctgacttcgc aaaccagcca tagcccggca
    10201 aacatgatgg tgatatctga aaagcgttgc accatagaga ttaacgatgc attggttttc
    10261 gctcgctcgc gcttttttag atttgtcatc gttgttcctg ttattagccc cttacccgca
    10321 gtggggtaag ggaagatccg acattactca ttcagcaacg tcagcagagt tcgcgttcgc
    10381 gcttccatca gcggcacatc accgcgcgat tccacattca accgcaccac cggttcggtg
    10441 ttggaggagc gcaggttaaa gcgccagtcg gcaaaggtca tgctgatgcc atcggtgcga
    10501 tccaccgcca gcgcctcacg gctaaaatgc tgttccacgc ggttaatcgc ctcaatgggg
    10561 tgcgccagtt tgctgttgat ctcaccgctt gccggaaacg cagccatccg gtcgcgcacc
    10621 agttcgccca gcgtttttcc tttcaggcac accagttcgg cgaccagcag ccacgggatc
    10681 atgccgctgt cgcagtaagc gaaatcacgg aagtaatggt gggcgctcat ttcgccaccg
    10741 tagatggcgt cttccttgcg catacgttct ttaataaagg cgtgtccggt tttcgacatc
    10801 accggcgtgc cgccagcagc agttaccaca tcaacggtgt tccaggagag acgtggatcg
    10861 tggatgatct tcgcgccggg atttttttcg aggaatgctt ctgccaacag gccgacaatg
    10921 tagtagccct caataaactg ccctttttcg tcaaacagga agcagcgatc aaaatcaccg
    10981 tcaaaggcaa tgcccatatc cgcgccgtgt ttgatgaccg cattgcgggt gtcgtcgcgg
    11041 cattctggca gtagcgggtt aggaataccg ttggggaaat tgccgtccgg cgtgttgtgt
    11101 actttgatta attccaccgg tgcgccgagg gctttaaatc gggcttcaat ggcgtccacc
    11161 accggacccg ccgcgccgtt cccggagtta atcaccagct tgagcggcgt gaggtttttg
    11221 acgttgatat aactgaacag gtgatcaacg taagcgtcac gcaggttgat ttgctgatag
    11281 cgaccgcgtt tggtttcatc gacgggagga aagtcgttgg cttctgccag acgctggacg
    11341 tcgcgcagtc cggtatcacc gctgatcggg cgagcccctt cgcgcaccag tttcatgccg
    11401 ttgtaatcca tcggattatg gctggcggta acttcaatgc cgccatccac accgagatgg
    11461 aacgtggcga aatagatctc ttcggtgccg gacatgccaa tatccagcac atcgacgccc
    11521 gcatcctgca aaccttttgc cagcgccagt tttaaggttt cgctggtgag gcggacatca
    11581 ccgcctaaca caatggtttt cggtttgaga aattcgccat aggcacgccc aatacgccag
    11641 gcaatatctt cattcagttc ttcgcctaat ttcccgcgaa tatcataggc tttaaagcag
    11701 gttaattttt tcatatcgtt accctttttc aggcaatagt tggccctgac cgaaacggcc
    11761 aattttgttt ttgtcgttat ttacaccgcg gtttcgcatt cattgcctga tgcgacgctg
    11821 ccgcgtctta tcaggcctac aaaaccgaac cgtaggtcgg ataaggcgtt cacgccgcat
    11881 ccgacaaaca gcgcctgatg cgacgtttac acccgtccgt agcgatccgc gaaacgcacc
    11941 acatcatcct cttcgagata agagccggag cgtacttcaa ttaaatcgag cggaattttc
    12001 cccgggtttt ccaggcagtg cgtcgccccc agcggaatat aaatggactc gttttcacca
    12061 agcagtttga tatcaccgtc aatggtgact tttgccgttc ccgcaaccac cacccagtgt
    12121 tccgcgcggt gatggtgcat ctgtaccgac aagccttcgc ccggtttcac ggtgatgcgt
    12181 ttcacctggt agcggtcgcc cgcgtcgata gagtcatatt tgccccacgg acgatacact
    12241 tcgcgatgca cccgatgctc atggcgacca tcggctttga tctgctcgac cacttttttc
    12301 acatcctgta ccgcgttacg gtcggcaatc agcaccgcat ctttggtctg caccactacc
    12361 agatctttca ccccgacggt ggtgaccagg ccagattcgg catacacata gctgttttca
    12421 gttttgtgat taatcacatc gccgtggcaa acgttgccct cggcggtgtg ggcgctgatc
    12481 tcccataatg aagaccagga accaacatcg ctccagcccg catccatcgg caccacaaca
    12541 gcatctgccg tacgttccat gaccgcgtaa tccaccgact cttccgggca ggcgagaaac
    12601 gcttcttcat ccacacgaat aaaatcaaga tccggatcga cggcgctcat cgctttttca
    12661 caggcatcga gaatatccgg gcgatatttt ttcagttctt cgagatagcg tccggcgcgg
    12721 aacaggaaca tgccgctgtt ccagtaatat tcgccgcttg ccacataggc ctgggcggtt
    12781 tccagattcg gtttttcgac aaactgcgcc acttcaaagg ccaccgtatc ctgctcaccc
    12841 gcagacactt caccgcgacg aatatagcca taaccggttt ctggtagatc cggcacaatg
    12901 ccaaaggtca ccagcttgcc cgcttcggca tatggcatgg cattacgcac ggcggcacgg
    12961 aacgcgtctt catcggcaat tacatgatcc gccgccagca ccagcattaa cgggtcgttc
    13021 tccgggctat gacgttttgc cgccagcgcc gccagcgcaa tggccggtgc agtgttacgc
    13081 cctgcaggtt cgagaataat gttctcggta agtttgttca gttgacgcag ctgttccgcg
    13141 acaataaagc ggtgctgctc attgcaaatc accaccgggc tttcgcactc cacgccgttc
    13201 aggcggcaga tggtggtttg cagcatggtg agatcgcctt tcaggcataa aaactgcttg
    13261 gggtaaagta cgcgggaaag cggccataag cggctaccgg agccacctgc catcacaact
    13321 ggatagagtt tcgactgcgc cataattatc cccgaatatc atttataaat tgacgtaaca
    13381 cgttctcttt atcgagcgtg cgttcggcat attcacgtgc caccgtgttg tacttgggca
    13441 gcaggagcgc ctgacgaatc cccgccacca gcgcctcgac cgattccggt tccacgcaaa
    13501 ccgcaatgcc cggaaaggtt tcgcaaagct gtcccagttc tgtgtgggcc tctgcggtaa
    13561 tcaccgcgtt accgcctact gccagaatat tggtcagttt cgacggcaat acggcatctg
    13621 ccgcgccgcg tttttgcacc accagatggc aatcgcccat cttcagcagt gcgggtaaag
    13681 cgtcatacga ttgcagcggg aaaaattgca tgttgcgcag tccacgctgc tgcgccattt
    13741 tttccagccg cgctttgccg ccgccctgcc cgacaatggc aaaaatcagc ggttcatcgc
    13801 gcaggcgatc ggcagcttca ataacgtttt ccagtccctg cttttcacca atattgccgg
    13861 agtaaagaat gatttttttg ttatccggca ggccaagctg gttacgaagg gcatcaacat
    13921 cggcgtctgc aacatgctga aaacgggcga tttccgacca gttggggaag aaaatgatgt
    13981 tatccgccgc cacgcctttt tcgatggctt tattcatcat cgaacgcgaa atcgtggaga
    14041 cgttatcgac gttatgcagt ccgctacgtt cgaacgccgt tgccagctgt gccactttgc
    14101 cgcctttgcc ttttccggca aggcccagcc ccagcatggc gtccacttcg taatcctgaa
    14161 tatgcagcac ggtacgcgca ccagagagtt tcgccagcag gcgcattccc ggcgtgcaaa
    14221 acagcgttgg caccacgccg ataatgcgat ccggcttcca gcgacgttgc gccatcaacg
    14281 ggaaaaaact gctgacggca aaactgccga gatgcaacag gcgtttcagg gtgctcggct
    14341 gttttggcac gtacagcggg cagcgccaca ccgtggcggc cccctcttct cgtttgtagc
    14401 gccaggcgga atagttctcg cccacctgcc actgcgggta gtaaggcggt gcggtaataa
    14461 cccgcacctc atgaccttgc gccgccagcc attccaccat ttctccggtg tatttgccga
    14521 tgccggttaa ctccggcgag tagttaatgc cgtagaccag tattttcata atccgggtac
    14581 tccggcacgc ttctcagcga gaaaataggc gcggctgtta gcgtgaacat tgtcactggc
    14641 gagcagtgcg tccggcgtca gccagcggta atcgtcatgc tgctcatccg gcagtaacag
    14701 ttcttcttcc gctactctga agcgaaaacc gagcaccaca tagtgagtgg tgaaatccgt
    14761 gccggagaag ttatcgtcat aaaagtgctg ccagacaccg taaaactggc ctgctgttat
    14821 cggcagacgc agccccagtt ccgccatcgt cagccgctca aatgcggctt ccagcgtttc
    14881 gtctttctgc acgcgccctc ccggcacaaa ccagtaaccc tgcgccgggc ggttggttct
    14941 tttgccaagc agaaactcgc cgcgactgtt ctcgacaata aagtcgagag agacaagcgg
    15001 tgtggagcgc actacagtgg caaagtcttc ctgacgtaaa aacatcattc cccccgaaag
    15061 cggtcttgat tctcaaggaa ccactggtaa gtgctggcaa gccccgcttc cagtgagatt
    15121 tcgtgatacc agccaagctg atgcaggcgc gtcacatcca gcagtttgcg cggcgtacca
    15181 tccggtttgc tggcatcaaa aaccacccga cctttgtaac ccaccacttt ggcgatggtt
    15241 tgcgccagct cgcggatagt gcagtcaacg cccgtaccaa cgttaatgtg cgacagcatc
    15301 ggctgggtgt tctccagcca gacttcatgc gccagctcca tgacatgaat gctcgccgcc
    15361 gccatgtcat cgacgtgcag gaattcacgc atcggtgtac cgctgcccca taccaccacg
    15421 tccggcgcat tctgtgccgt cgcctcgtgg aagcgacgca gcaatgctgg gatcacatgc
    15481 gagttactcg ggtggaagtt gtcatgcggg ccatacaggt tggtcggcat gactgagcgg
    15541 taatcgcgtc cgtactggcg gttgtatgat tcgcacagtt tgatcccggc gattttggca
    15601 atggcgtaag gctcgttggt cggctccagc gtgccctgca ataactcgct ttctgccatc
    15661 ggctgttttg ccagtttcgg gtagatacag gacgatccaa gaaacagcag tttgttcacg
    15721 tcgttctgat gcgcggcgtg aatgatgttg ctctcaatca tcatgttctg gtagatgaaa
    15781 tccgccggat aggtgttgtt ggcaacaatg ccgcccactt tcgccgccgc cagatagacc
    15841 tggtcaatgc gttcgctggc aaagaaatca tgcaccgcgc ggctgtccag caggttcagc
    15901 tcgtcgcggg tgcgtaatac cagttccaca tcaccgcgct gttcgagctg ccgcctgatg
    15961 gcagaaccga ccattccgcg atggccagcg ataaaaatgc gttgtctact catgcttatg
    16021 actccagcgc gatcgccacg tcgtagccgt gagatttcag cagagagtgt tttttcgccg
    16081 cttcgaggtc attagccacc atttcagaca ccatctctct gagggtgatt tccggtttcc
    16141 agcccagttt ttcgtgcgct ttggtcgggt cgccgagcag cgtttcaact tcagccggac
    16201 ggaagtaacg cggatcaacg gcgataatca catcacccgg tttaacgccc ggcgcgtcat
    16261 gcccggtgac ggaaaccaca atgcccttct cttcaacacc cgtgccttca aagcgcagtt
    16321 tgatgcccag ctgtgccgct gccatttcca cgaactgacg cacggagtac tgaacgccgg
    16381 tcgcgataac gaaatcttcc ggctgttcct gttgcagcat catccactgc atttttacgt
    16441 agtctttggc gtggccccag tcacgcaggg aatccatatt gccgaggtac aggcacgact
    16501 ccagcccctg ggcgatgttg gcgattgcgc gggtgatttt gcgggtaacg aaggtttcgc
    16561 cgcggcgcgg ggattcatgg ttgaagagaa ttccgttaca agcgtacatg ccgtaggatt
    16621 cgcggtagtt aacggtgatc cagtaggcgt acagtttggc gaccgcatac ggagatcgcg
    16681 ggtagaacgg cgtggtttct ttctgcggaa tttcctgcac cagaccgtac agttcagagg
    16741 tggaagcctg atagaaacgg gttttctttt ccagaccgag gaagcggatc gcctcgagca
    16801 ggcgcagcgt acccatcgca tcgacgtctg cggtatattc cggtgactca aaagaaaccg
    16861 caacgtggct cattgcgccc aggttgtaca cttcatccgg ctgcacttca cgcaaaatgc
    16921 gcgtcaggtt ggaggtatca ctcaggtcgc cataatgcag atggaatttc gggttgcagg
    16981 tgtgcggatc ctgataaatg tgatccacgc gctcggtgtt gaatgacgat gcacgacgct
    17041 taataccatg cacctcgtaa cctttttcca gcagaaactc tgccaggtaa gaaccgtctt
    17101 gtccggttac accggtgatg agagcgactt ttgacatgtc ttattcctct gtatttttga
    17161 atttattcag tttcaacgcg ttcgcgtatc accactgcgg gattcccccg gcaaaccaca
    17221 tttgccggaa gcgatttaaa aacactgctt cgtgcaccca cgacggtgcc gtcgccgatt
    17281 gtgacgcctg gggcaacaaa gacatcggtt gccagccagc atttctcgcc aatcacaata
    17341 ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt ggctcccggt gcataaataa
    17401 cttttttgcg atatcaccga atgtgcgcca atggttattt caccgagggt atataaattg
    17461 acctcatcgc cgacccacgc gtaatcacct aaggttaatt tccacggata ggtaattttt
    17521 actgacggac gaataactac gttttttcct atttttgctc cgaataaacg taataaaaaa
    17581 gcccgccagc gatacaatac ttgtggcgac caggcaaata ttgttgcctg tactgcccac
    17641 cataattgca ctttaatagc gttgccgccc cggaaccctt tcggcaccga gaatccactt
    17701 aaatcctgca tcgttttccc ttatattcag actttgttat ataaggcttt cgtctttgag
    17761 gtagtacgtt ggcgtaaatg ccaggataat tcagcccaaa agccaggtac atgtaatatt
    17821 tgtcgttgga cttttttcgc gtcagcacac aattccatat tattggtggt agatacccca
    17881 cccatggaaa attcagacac caggccattg agttttttaa atgcataacc ggctttatac
    17941 attttggctg ccagcgcgta gtcggaagaa actttatatt ccaggtcata acgccatttc
    18001 ttcaagccgg ataccgggaa aaatatcgcc tgatgactgg cgggcaggct gtgataaata
    18061 taccagcccg gtttggcgct acgtttaatt ttatgcccgt cgccaaaatc cagcagcgca
    18121 tcgccggtga tcatcacgtt atctttttgc atttttaact tacggacaaa atttgcggca
    18181 ttctgatgaa aaatatcgcc cgaattgaga aacaacgcga acttgccttg tgccatcgta
    18241 atgcctttgt tcatggcgtc atagataccg ttatctggct cgctgacaaa gcgtaggtta
    18301 tagataccat tgagattttc cagatactca cgggtgccgt cattggaacc gccatcgaca
    18361 acaatccatt cgaagctgat atcttccgcc tgcgccagat gcgccagcga ggcatgtgtt
    18421 ttgactatcc cttcgaggtt acgaaacgcg acagtgatta tgctaagcaa catgttctta
    18481 ttcttacctc gtaatattta acgcttttcg caaaataaac ggacagacga ttaaaaatgc
    18541 atattccggg ctaaatatcg aaccggtaaa aaacagcgat accggagtaa aaagatataa
    18601 ttgcacgcga aaattgcggt tatcaccaaa agcgttgatc atcattttta tcactttccc
    18661 catgtaccac agcgataaaa acaccgcgaa ccaggaaaaa taaataatca gcagatacaa
    18721 accattgtct atggtttttc cgacatccgc accgttaaat attccgaatg atgcgacata
    18781 ttcataaagt gagccaaatc tgactacacc gtcaatatgg gtcaaagaat aaccgaccat
    18841 caccagcgga ccgacaatac gataatacga tgacgatcct tccgtaccta aatcgcccag
    18901 acgggtggaa atataaggaa acgcgattac cacaccaacc aggaatacag ccagagaaat
    18961 caatgctaac ggtaactttt tcttaatcgc ctctttattc agatattgga atgcccactc
    19021 cagcaaataa aacaggataa aggtcataac ccctgaaaac gatccggata atattatccc
    19081 tgcgagaatc atagcatcgg ttttaggcgt tttgatacca aactgtttga tgctgagcca
    19141 aattgagatt aatgccagag cgaaaaatgc cggttcgaaa taaagtgctg tcgtgcgctt
    19201 gccgccgaat ttgatgaaat tcagcacata gctgttgctg taaatcagat atttcgaaat
    19261 cgactccata atactgctgc cgccggtgag gataatttgc gccatctcca ccgccgccag
    19321 cgccaccacc agcccgacca ccagataaaa gaaacgtaat atcttgcgat ggttgtgtgg
    19381 cgaaattgtt ttaaaacgaa tactccacac catgccaata atgatcacaa tatagacaaa
    19441 cagcatggtt gaagtgacgt atttaccggc atccagcgac tgaccaaaca ggtagttaaa
    19501 cgccgtgagt cccgcaccaa tccctaaggc aatcatcaat tttttaacgc tgatgcgttc
    19561 taaaaacagc agtaacaaga caggtaaaaa agtgacgata gtgatgggga aactttcgcc
    19621 aagctgggcg attttgacgt taaccagcag gtagatcagc ggcagcagca ggtagctaca
    19681 gattctgata gaagttgaca tactcctcca gcatctgttg tccactgtag gcggcgcggc
    19741 tgcgttggct gaactcagcc agcgtggtac caaatatcgc ctgcgcgatt tccggtttgc
    19801 ttaactgcac cagttgcagc acctcttctt cactgacggt tttaccgccg gatttttgca
    19861 gcacttcacg cgccgcatcg ctatgggtgg caatcaccgg cacgccaatc gatagcgcct
    19921 cacacaaaat cagcgggtag ttatcgacgc gagaactgaa caccagcgca tccatctgat
    19981 tgagcgcgct catcagctta cgtttgtcgg tttcaaagcc gtgattaacc acgttaccag
    20041 cggtgaacgg cgagaactta ccaaaggtat gcagttcgat tttgtcgccc agcgccatca
    20101 tctcacgcac cagttgctgg ttagttttgc cgtcgtaacg caggtcatgc gccaccaccg
    20161 cgattttcgg cttaccctgg gtttcgcgca ccggaggcag atccgccaga atcgcttcgg
    20221 ttgccatatc aataccgtta ttgataatcc ggcaacgccc tggaccgtac aggctattga
    20281 aagcgtcagc cacatgctgg ctgggggaaa taaactgaca gcccagcgcc agcatctcac
    20341 ggaataactg gcgtttgccc gccaccagtt ggtgtgcgcg atcaatcttc accggcggat
    20401 aattatttaa ggtcgggcat ttctggcagc ccgttttcca gccttcacaa ccgtcggtaa
    20461 aggcgcagcg tccggtaacg ctccagtggt cgtgcagcgt ccagaccaga gtgacgtccg
    20521 gtttatggtt tttcaccttt tcgcaaaagc gcaccacgct cttaagattt agccagtagc
    20581 tgtgcagcac atgaaaatgc aggaccaccg gacccggtgt acgagtaatg gtgcgatata
    20641 actcattgaa attgccaaac agatcgcgat taaacagacg aaacaggaca atattcgcca
    20701 ttgcagtcat ccgcggcgta tgtttgatga cctgcggata gttttgatgg ctgacgctct
    20761 ctttgccgcc tttgccgtaa ccgtagacaa aatgtgacgc tagcccctgt tgcagcgcac
    20821 gctggtggag atctaacgcc acacctgctg ccccgccttc cgccagtcgc acattaaatt
    20881 gcaaaatatt catttaatta ccttcactcg cgctttttct cccaccacca gcgcgttgtc
    20941 cgggacagag tcgagcacca cgctgcccgc acccacggtg acgttgttac caagcgtgat
    21001 atcaccaaga ataataacgt tggcaccgag ttcgacgccg ttgccaatat gtggacatgc
    21061 catgttatcg gcaccacgat tgccgatagt gacgccgtgg cgaatggtga aatcatcccc
    21121 cgccaccacg tttttattga tcactacggc gtaaccgtga tggatagtaa agcggcggcc
    21181 aatggtcgcg gcagcctgga tttcataacc gaaaaagcat tcggtgataa tgcgatacag
    21241 caccagcaga ggggccgccc aaatattgtt gaggacgttc tttttgcgcc acaccgaaca
    21301 aaaatgagcg acgcgatagg ctagaaccat gcagcacgga cgtaaactcc agctgttggc
    21361 gcgcagatct tccagcatct ttagcgcccc cgaataccat cagccagacg tttgccgtta
    21421 cgcaccgaca gcagcgtcag caaagtgcgc caggtcatac gtttattgcg gatctggtag
    21481 agggtaaaca gctgatattt tttgctggcg cggtcgaatt tgtctttgtg cttgcggtaa
    21541 aagtggaagt atccggagaa ttttttcggt gacgaggtga tctgcatttc accgtgattg
    21601 atatgcagga tctgcgtcgc ctcttccact ttccacggtt cgccatactc caccaccatc
    21661 cgcaggaaga tgtcgtaatc ctgtgccgct ttcagttcgg tatcgaacaa gcactctttg
    21721 aaacgccacg cccaggtaaa aacctgattg ccaatgatat tgcgtttata gaacaggcgg
    21781 cgtgagtacg gcgatttggg atacagtggt aagcttgctg gttgggaata aacttctccc
    21841 tggcatacgt agtcgttagc gtacaaaaag gcgtgagtga ccagttgctg tttatgggcg
    21901 aggaagacgc tcaggcggtt gggcgtccat tcatcatcgt catcgatccc ggtaatgtat
    21961 tccccttgcg ccagcataat cgcctggtta cgtaccgcgc acgccccgct gttaatgtcg
    22021 ttgtgaatgt aggtgatacg cggatcgttg agggcagtga cgtactgttg cagttgttcc
    22081 caggaggtgg agcaatcatc cacgatgatc atctcccagt tgctgtagtc ctggcgcagg
    22141 accgatttta tcgcccgaat cgccagttgt tggcggttcc aggtcggcat atagattgaa
    22201 atcagcggat tgtctttcat aggttgttct cccggagtat cgagatgttc tgccggttta
    22261 taagcttgtt tacgggttct gttgccggat gcggcgtgag cgccttatcc ggcctacgga
    22321 gctggtttgc ttgccggatg cggcgtgaag gtaggtcgga taagatgcgt cagcatcgca
    22381 tccgacagcg gatgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa
    22441 tcctgatacg cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata
    22501 ccgttttgct caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa
    22561 cgcgccacca ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc
    22621 agaatcggcg gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt
    22681 tcggcaaagc gttcgctcat caacagttca gaaggatttg gcggtacctg accgcgcggg
    22741 atcaggtcaa atttggcaat agaggtcggt ttagctgcgg tagtaatatc gccctggcca
    22801 atcagaattt ccgacaggcc attgacgtta ttggtaccca acagctcgtg ggtgtagcct
    22861 ttgcgcatat cgcagtcgat caacaacacc cgtttattgg tctgactgat caccgccgcc
    22921 aggttggcgc agacaaaggt tttaccgatt gacgggctca cccctgtcat catcaacaca
    22981 ttgttctgcg cctgcatcat cgcgaagtgc agactggtac gcagactgcg gatggcttca
    23041 atcgccagat cggttggatt ccccaccgcc agtagctggc tctgtttata gcgtttaatc
    23101 cctttgatgg ttttgacgct atcgcgcgct ttctgccatt ccgacaacgg gatgctggca
    23161 tagacgctga taccatgttc ttccagcacc tgcgggcttt cgatgccgcg attaaacaac
    23221 gagcgcagca gcacacccac gatagagagc atcaggccga ggataattgc gccgaggata
    23281 atcagtcctt tcttcggttt cagcacgcca ggctgggtga ttgccgggtc aacaatgcgc
    23341 acatcgccga cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc
    23401 atatagacct gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctgggtt
    23461 ttcggcatcg ccgttacgcg accattaagt ttggcttttt cgtcttccag cgcctgacgt
    23521 ttctccagca gcgtgcggta cgcggggtga actttggtgt acagcttgga gatttccgcc
    23581 tctttaaagg tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct
    23641 ttcgcttcca gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt
    23701 tcggcaacat cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt
    23761 ttcgacgctt ccgccgattt gcgctcaata ttttgttcct gatagttacg ggcgatgctg
    23821 ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct
    23881 gcgtctttgc cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac
    23941 gtggagtatt tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc
    24001 agcgtgacgc cttctttttt cagaatctgg cccgcttgcc cacgggcgct aaagccgcca
    24061 tcactgctca gagtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctggtccgcc
    24121 atctctttcg ggcggttaaa ggtggtcact ttcaccgtct cgttctgacg tcccatcagg
    24181 cgatcccaac ccgcaccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtct
    24241 agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc
    24301 gatgcaggcg gtttgttggc taatgccgag ccaatgtcct gcactaacga attgccgctg
    24361 ttttgttcga tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa
    24421 accacagcac agagggcaaa tacagcggtg atgccaatca cccaccagcg cgcttcaatg
    24481 acggtgccga ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt
    24541 tgttttactt tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc
    24601 gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgcgatacg
    24661 gatcggggat ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct
    24721 caggtgccat ctcgcataag cgttcgatat ggcgcttttc catggtcaaa atcaggtcat
    24781 agttgcgaca caggcggcgg ctgatttgcc gtgcacagtg accttccaga gacagttgat
    24841 gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc
    24901 cagcggactc cactttcagc tccgggtgat aacgttgcag taagcgttcc gccgtcgggg
    24961 aacggcaaat attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt
    25021 atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg
    25081 attgatcaga cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg
    25141 ttgcaggcgg aattctgttg ccatcaccag cgacgtcgca tcggacatat ccagctggta
    25201 gatattggca atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat
    25261 gccgctggcg ttggaggtgg tcatgtcgat accttcggca ttgcccaggg cttcagtcag
    25321 ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa
    25381 cactttcaga tcatcattac gcggcacgta gagaatatcg ccggggtaaa gcagtcggtt
    25441 ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa atgcgttctt cacgaccatt
    25501 gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat
    25561 ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg
    25621 acctgagata taggcctttt gcgagcggaa ggcggcgata ttaacatcca cctgcgggtc
    25681 agcgatgtac gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc
    25741 gactacgtgg accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt
    25801 gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg gtgagttccg ggtgatccca
    25861 gacggtgaca ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcact
    25921 ttccagcgtc atattggggc gcgctacatt cgggcgtgga cgtaattgat caatcaggcg
    25981 cggggtcaac ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt
    26041 gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat
    26101 caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt
    26161 atcactttgg cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt
    26221 accttgcagt taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat
    26281 ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc
    26341 ccctggcttt ttagctacca atacactgat ttagcttaat ttttcaaacc ctctctgcat
    26401 acaatcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta
    26461 tggtaattga atattggctc tccaataatg caggaggaag tgttacagct aacggaatag
    26521 caggcaaggt aacaattcgg caattggcta tttttaagaa ttatattaag tgtcattcaa
    26581 tatggttttt aggagtttct ttaggttgac aatatttaat attgcgcctc cacatgcgat
    26641 atttcttaaa taatgtttta ttattaccac ttttaattca gggataatgt gaggttatta
    26701 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
    26761 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
    26821 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
    26881 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga
    26941 ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctggcgtc
    27001 aatctcctgg ctggtcaccc tgacgcaacc gctgttcagc ttccgctcgt ttacctttag
    27061 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
    27121 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
    27181 gttctggggg gtggtgacac aaattgtggt actggacgcc atcttctcac tcgactcagt
    27241 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
    27301 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
    27361 catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
    27421 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat
    27481 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
    27541 gctacgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc
    27601 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
    27661 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ctgtcagcag
    27721 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagacgagat
    27781 ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc
    27841 agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgtggcga
    27901 accgctcaac ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt
    27961 gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga
    28021 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa
    28081 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
    28141 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
    28201 gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat atttacagcg
    28261 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
    28321 ggaaagccat cgcgtgcaga aggtgcagat tattccgctg cataaggatg gcgagttgga
    28381 gtacgaggtg tgatggtatc ggtcatttta cccgatagcc gaaaggaata agatgaggga
    28441 accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc
    28501 caccctggca cgttcaccgg cttgagcgtt ccatagtagt gaggaacatc aagtgaacgg
    28561 aacagccttc gatcttacat attcagaata taatggtgcc gccgcgaacg cctgatgcga
    28621 cgctttacgc gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca
    28681 gcaacttctt cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca
    28741 ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcagactgt
    28801 aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat
    28861 caatcagttt gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc
    28921 aggtttgatc tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac
    28981 cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt
    29041 caagccgcgt cacgttatca aagttttcag cggccttcac atcaccgcca ttacgctcta
    29101 ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg gtcatttcga
    29161 catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga
    29221 aatcaccagc cagtgacatt tttccggtca gcgaaatggg gtagttaaag gcattcagaa
    29281 tggtaccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga tttattgatg
    29341 tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt aagcttgccc tgcagttgag
    29401 taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc
    29461 cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct tgcagtccct
    29521 gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaatgtt gattggctct
    29581 gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc attaagcggg atgaggttgt
    29641 caagattcag ttgcgggaat tgcagtctca gctgccattc aggtttctct gtcagcgtga
    29701 cctgtgcttg cccgctcagc gtgctgtcat tggcggtcaa gctgatttgg ttaaatgaaa
    29761 ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga ataccttgtt
    29821 ttggtaaatc tgcaccctgc aactgccagt taatttgttc aatagccgcc gttaaatcat
    29881 gcggataatc agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc
    29941 gattaacccg cccggagaac tcaaatgaac cacgatgttg gggatcttgt tccatttgca
    30001 ggcgaatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc agcacgctgt
    30061 ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca
    30121 aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg
    30181 tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta
    30241 gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc
    30301 cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac
    30361 gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat
    30421 agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca
    30481 cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga
    30541 attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt
    30601 cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa
    30661 ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa
    30721 tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac
    30781 gcgccaaaga ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact
    30841 ccagcgtcac cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga
    30901 gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc
    30961 tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaagcgta
    31021 catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc
    31081 cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg
    31141 cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac tgaacggtac
    31201 acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg
    31261 atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg
    31321 aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa
    31381 cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta
    31441 tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg
    31501 ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt
    31561 tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc
    31621 tcaagcagca ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct
    31681 tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt
    31741 tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg
    31801 gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat
    31861 ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat
    31921 tatagggatt tcatccgcct gataccagtc gaatagcgct gccgcgcgct cagagtttat
    31981 tgttgacgaa gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat
    32041 gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcgcatata
    32101 aaatgtaaaa cttttgtact agcataaaca cagaaacgaa tactggcgac ctggtcttgc
    32161 ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc cccacccgct
    32221 gcttcacctg gtcagtttag gtttagtctc gtttatcttt acccttttct cgcttgagct
    32281 ttcgcagttt ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc
    32341 gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctgagctgtt cgctgggaaa
    32401 tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat
    32461 caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa
    32521 ccccttgcaa aatctggctg actggctgcg tctggcactc ggcagcgcca ttgttccgcc
    32581 tctgttgggg gggggtctgg ttgtcctgct gacggccgga gacgatcctc tcagggcatt
    32641 tttgatatgg gtactgtcag aatccatcgg cgctctggca ctggtgccgc tgggattgtt
    32701 atttaaacca cactatctgc tgcgccatcg caacccacgg ttgctttttg agtcgctgct
    32761 cacgttagcc atcacactga cgttaagctg gctttcgatg ctgtatctgc cgtggccttt
    32821 tactttcatt attgtgctgt tgatgtggag cgccgtgcgc ctgccacgaa tggaagcctt
    32881 tttgatcttc cttaccacgg tgatgatggt gtcgctgatg atggccgcgg atccctccct
    32941 gcttgctacg ccgcgtacat acctgatgag ccatatgccg tggctaccgt ttttgctgat
    33001 cctgctgccc gccaacatca tgacgatggt gatgtatgcc tttcgtgcgg aacgcaaaca
    33061 catttccgaa agcgaaaccc gttttcggaa cgccatggaa tattccgcta tcggtatggc
    33121 gttagtgggc accgagggac aatggctgca aaccaacaaa gcgctctgcc agtttctcgg
    33181 gtacagtcag gaagagctgc gcggactcac ctttcagcaa ctgacctggc cggaggatct
    33241 caataaagat ctccaacagg ttgaaaagct gataagcggt gaaataaaca cctattcaat
    33301 ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg gcgttgcttg ccgtctcact
    33361 ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag acattaacga
    33421 gctaaaacgc accgaacagg tgaatcagca actgatggag cgcatcacgc tggctaacga
    33481 agcgggcggg attggcatct gggagtggga gttgaagccg aatattttta gctgggataa
    33541 gcggatgttc gagctgtatg aaattcctcc acatatcaaa ccgaactggc aggtgtggta
    33601 cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa gtgattcgtg attcgttgca
    33661 atcacgctcg ccctttaaac tggaatttcg cattaccgta aaagacggta ttcgccatat
    33721 ccgcgccctc gccaaccggg tactgaataa agaaggcgaa gtcgaacgcc tgctcggcat
    33781 taatatggat atgactgagg ttaaacagct taacgaggca ttgtttcagg aaaaagagcg
    33841 cctgcacata acgctggact ccatcggcga agccgtggtc tgtattgata tggcaatgaa
    33901 aattaccttt atgaatccag tggcggagaa gatgagcggc tggacgcagg aagaagcatt
    33961 aggtgttccg ctcctgacgg tgttgcatat tacctttggc gacaatggtc ccctgatgga
    34021 gaacatttac agtgccgaca cctcacgttc cgcgattgaa caagatgtgg tgttgcactg
    34081 ccggagcggc ggcagctacg acgtgcatta cagtattacg ccgttaagta ctctggacgg
    34141 cagcaatatt ggttcggttc tggtgattca ggacgtcacc gaatcacgca aaatgctacg
    34201 ccagctgagc tacagcgcct cccatgatgc actaacgcat ctcgccaatc gcgccagttt
    34261 tgagaagcaa ctgcgtatcc tgctgcaaac ggtaaacagt acacatcagc gacatgccct
    34321 ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac agcgccgggc acgccgctgg
    34381 cgacgcttta ctgcgcgaac tggcgtcgtt gatgctgagc atgctgcgct ccagcgacgt
    34441 gctggcgcgg ctcggcggtg atgaatttgg tctgctgctg ccagactgca atgtcgaaag
    34501 tgcgcgtttt atcgctacac gtattatcag tgctgtgaat gactatcact ttatctggga
    34561 aggacgtgta catcgggtag gtgccagtgc cgggattacc ttgattgatg acaacaatca
    34621 tcaggcggct gaagtgatgt cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg
    34681 ccgtggccgg gtgacgattt acgaaccgca gcaagctgcc gcacatagcg aacggacggc
    34741 gatgtcgctt gatgaacagt ggcggatgat taaagagaat cagttgatga tgatcgccca
    34801 cggtgtcgct tcgccgcgga tcccggaagc gcgtaatttg tggctgattt cgcttaaact
    34861 ctggagttgc gaaggcgaga ttattgatga acaaaaattt cgtcgtagct tcagcgatcc
    34921 ggcacttagc catgctcttg accgacgggt attccacgaa tttttccagc tggccgcaaa
    34981 agcggttgcc agtaaaggca taagcatcgc cctccccctt tccgttgccg gtttgagtag
    35041 cgccacgctg gtgaatgatc tgcttgagca gctggaaaat agccctctac caccacggtt
    35101 attacatctg attattccgg cagacgcgat tttagatcac gcagaaagcg tgcaaaaact
    35161 gcggctggcg ggatgtcgga tagtgctcag ccaggtgggc cgcgatctgc aaatcttcaa
    35221 ctcgctgaaa gcgaatatgg cagattacct gctacttgat ggtgagttat gcgccaacgt
    35281 gcagggtaat ttgatggatg agatgctgat tacgattatt caggggcacg ctcagcgact
    35341 cgggatgaaa accatcgccg ggccagtcgt tttaccctta gtgatggata cgctttctgg
    35401 catcggcgtc gatctgattt atggtgatgt gattgccgat gcccaaccgc tggatttgct
    35461 ggtgaatagc agttatttcg cgattaactg agggagcgtt tgcccattgc ctgatgcgac
    35521 gcatcaggcc tacaattcgt ccgttccgtc tggttgccag ccttccgtat accagatatg
    35581 caacagcgca taagaacgcc agggcttcca gcgctcggca taacggcgga tttgtgccgg
    35641 tgtcatcccc ggaaatcgct gtttaatcag ataatcatcc ggcagaaaaa catctttcgc
    35701 ctgccagcca cgcaaagcga aataattcgc cgtccagcgc ccgatacccg gaaaagtttg
    35761 cagcgttttc atcgcctgct ccacatcgcc cggaattgtc attggtaagc tgccctccag
    35821 cgccgcattt gccagatgaa tcagtgcctc tgcccgtttc aacggcatgc ctaacgcttt
    35881 taatgcctgc gggtcggctg ctgccagccg ctgaggcgtg gggaagcaga tatactccgg
    35941 aaaatcatcc agccgttcgc cataaagctg taccactttg gcagtcaatt ttgccgccat
    36001 cgccacgctc accagttggc ctaaaatcgc ccgcacaccc tgctcaaaag catcaacaca
    36061 gccgggtaaa cgcaatccgg gccgcgccgc gcctaacttg cccaacgcac cgttaacaat
    36121 ctgcgggtta cattgcagat caagcaggcg gctcattttc gccagacact cagcggcaac
    36181 aggttctaaa cctgcactta aatttatgtg cagagtatga cgggccatat ccggaatagc
    36241 agtcactacg ccgcgatatt cgcccaccgc cagactacgg gcataataat cgtcagcgac
    36301 cgtttccacg ccactcactg cacgggcggc gagaaatccc aacatccacg accagtcata
    36361 cggcggctgc cagttcaggg tatacatcgc atctccttat tcatccgctt tcagcataaa
    36421 cgttattcag acgctacgct ttgctttcat attccggttg tcgcgacggc aacatttcgc
    36481 taaagtcacg ccccttcttc accggcatgg ggattaattc gtgtttattg gttttgatta
    36541 cggtacagca aactgttcag tggcggtcat gcgtgacggt aaaccgcaat tactgaaaat
    36601 ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg ccaacgcgtg aagcagtaag
    36661 tgaatggctg taccgccatc atgatgttcc ggcagacgac gatgaaacgc aggcgctgtt
    36721 acgtcgggcg attcgttata accgcgaaga agatatcgat gttacggcga aaagcgtgca
    36781 gttcggtctt tcctcactgg cacagtacat tgatgatcca gaagaagtgt ggtttgtgaa
    36841 atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg cagcaggtag cgctgtttga
    36901 ggatctggtc tgcgcaatga tgttgcacat tcgccagcag gcgcaggcgc agctgccaga
    36961 agcgattact caggcggtga ttggtcgccc gatcaacttc caggggctgg gcggcgatga
    37021 agcaaacgcc caggcgcaag ggattctgga acgcgcggcg aagcgtgccg gattcaggga
    37081 cgtggtattc cagtacgagc cggtcgcggc tgggctggat tacgaagcca ccttgcagga
    37141 agaaaaacgg gtgctggtgg tggatatcgg cggtggtacg actgactgtt cattgctgct
    37201 gatggggccg cagtggcgtt cgcgtctcga tcgtgaagcc agtctgctgg gtcacagtgg
    37261 ttgtcgtatt ggcggtaacg atctggatat cgccctggcg tttaaaaacc tgatgccact
    37321 gctgggtatg ggtggcgaaa ccgaaaaagg catcgccctg ccgatcctgc cgtggtggaa
    37381 tgcggttgcc atcaacgacg tacctgcgca gagtgatttc tacagtagtg ccaacggtcg
    37441 tctgcttaac gatctggtac gcgatgcccg cgaaccggaa aaagtggccc tgttacagaa
    37501 agtctggcgt cagcgtttaa gctatcgcct ggtgcgtagc gcagaagagt gcaaaattgc
    37561 tctttcaagc gtagcggaaa cccgcgcctc actgccgttt atcagcaatg aactggctac
    37621 gctgattagc cagcgagggc tggaaagcgc cctcagccag ccactggcgc ggattctgga
    37681 acaggtgcaa ctggccctgg ataacgccca ggaaaaaccg gacgttatct acctgaccgg
    37741 cggtagcgcc cgttctccgc tgattaaaaa agcgctggca gaacagttgc cgggcattcc
    37801 gattgcaggc ggcgatgact ttggctccgt caccgccggg ctggcacgct gggcggaagt
    37861 ggtgtttcgt taattgaaaa atggctcagc agcggcttaa atctgcctgc gcctgagcca
    37921 gtttttctgc tgcctgacgt aactgttcca tcaacggcat ggtccgtgct ggagcatgaa
    37981 ggcggaatcg ctgcaactct gtcgcgccga ctgtcaacgc cgattccagc cgatgtcgtt
    38041 tggcggtcat tttcgccatt accgcttgtc tgtctgctgg cgttatcgct tcattcggcc
    38101 tgaacacaaa acggcgttca cagctcgctt tccagtcgat aaccgcctgg gtcagatggt
    38161 cgccaaaccc tttcacttgc ttaacgccac gacgggtaac atctgcggct gtttcaatac
    38221 caaaagaccg taacgccgct ttacgcgcag ggccaacgcc gggaatagag gcaacatcaa
    38281 taaaaaatcc ctccagaaac ttctgcttct gccgttccct tgcagtgtca tgaagtgctg
    38341 ccagagcgcg tttctcttct tccggtaacc cgagaatttc gtccttcatt ttttcgagca
    38401 tcgtccgttt ggcgataaaa ccttccaggc cgcctacctg ctggatctgg cggactaaat
    38461 ggtcatagtc cattttcgca cgattaaatg cctcacggcg ttgctgaact tctgctttgt
    38521 acgctttgct tgtcagactg ccaataatcc agatagccgc cagcacagga accaaaataa
    38581 tataacgcgg ttctgcctga aggccgcaaa gcagcagcga taatgctgac agtgcgatct
    38641 caagcagaat gatgtattcg cgccgtaaca ggcctaaagg aagcggcctg ccagtcggtt
    38701 ggaaatgatc gggtaatggc agttgcaatg ctggcggtgc tactgacgcc atcaccatcg
    38761 cccagacttt tgccagcaca aaatccccgc cggtggtaat gacctcttcg ccgagatcaa
    38821 taaaatagat aacaccttga ttatccagcg cacaccatgg gcagtcagcc aaatgaccgg
    38881 gataaacatg cattgccgaa acggtacatt tctttaactg ttggcgcaga gaatcaagtg
    38941 ccgctaccca cgctttagcc gtcggacgcc cggttgccac gccactttcc gtgaatgcct
    39001 gctgaaacat ggcttcaaca tcactcggta acatcgatag cggaatcgat cgtggcggcg
    39061 gttttaaacc acgtcgctga ttatctgacg cgtaggcata acggaaatgg gtaatatccg
    39121 tctccagcgc attacccgca tcagagataa gcggcacacc agaataagga tgccggccac
    39181 caaacaagac gtgaaaaatc agcaacgcaa ggccaaaatt atcgtgattt tctgtgcgtt
    39241 caaagccgac aaatgacgac aaggtttgta gctctggcgg cgtaaaatgc gacacgccga
    39301 cttcgcataa atgcagtgtg ccattggcgt taatctgaaa ggagtcgcta tcaatcagta
    39361 ccactttgct gtcgcgacct accataaaac tgttctggtt tacgtcaccc acgacgtgcc
    39421 cgtgctcgtg aaccgtagca aaagatgaag caatattgcg cgcaacatag agtagaaaat
    39481 cccacgcaca atgagggtaa cgttgacgac gatgtgccgg gctatagatc atatgaatcg
    39541 gttctttacc agaaactttt ggcatcataa aaccgataac ttttccgcct cgtccaccgt
    39601 gaagcgttgc ctgcggccag gcgacataat tcaacaattg cgcgtcagct gtcgcagcca
    39661 taaaggcaag tttgtcctgt tttaaggcgg gtggcggcgt gtgataaatc ttggcgacgc
    39721 tatcgacaaa ctcattgata tcataaaccg cgccttcacc gcctttgccc agttcacggc
    39781 ctggcgtaac gcactcacca gtagcagtat ataaagtggg tttcatggta accacagcgc
    39841 cagtgctaac gttttatcat catcggtacg ctcgtttacc gctgggctgg acaaaaactg
    39901 ctttaacaat tcaggtaata aatcgagttg ttcctgcgtt gctgacgcca gtccattgaa
    39961 aaacggggta aagaaaggca catgcggaga attatccaac atatttaacg ccagtcgctg
    40021 gataccatcc gtaaatgctg caactttatg cacacgctca gtgctggtga aagtttccag
    40081 tctggaaacg gcatcttcat cggtaatgaa gtgcgtcata ttggcatatt cacccaccat
    40141 cggcgtaagc ggcaattgca gaccgtgacc aaaatccacc accacgccgc catcgccaat
    40201 ctgcataatc aaggtgccat tagccgatga aatcaatccc agaaacgtgc aggcaaaatc
    40261 gcgaaccgct aattctttgg cctctgcctc ggcaaaaagt cgctgacgaa cggtcagcac
    40321 aatatccgtg gcgaggatat cattaagccc caattcccca ccctgcactt tttgcgacat
    40381 ataagccatc gcttcattga cggcgagcat cgcgccttcg ccaccctgcg agacgctgcc
    40441 tgcaccatca gcaagaaaca tcaccagtaa tggttgctga tcgtttaacc aggcaacctg
    40501 catctggcag gcatcctgac aaggtaaatc agcgctaatg tgcgacgttc caacagcgga
    40561 tgcataaacc agacgccagc tcacactgac gtccagcctt ttggcgcttc cagcacaact
    40621 tccgttcccg gcgtggagcg ggaaaccgaa tgcagcgaac tggataacca gctaaacagt
    40681 tcacggaatt gtaatccttg cagaggcaaa ggctgacgaa cgctgatttg tgccagcgtc
    40741 ttcatatccg ccccctgaac agcgatggaa aagaaggcaa acttcttatc ttcttccccc
    40801 tgaaacactt tgttggcggc agcctgccac tcatcggttg gagcgccatc ggtgatcagg
    40861 aaaatccacg ggcgataata cgaaatacca tttgcgcggt actcacgttt cctctcttca
    40921 accatatcca gggctttggt aatggcggcc cccattggcg tatccccctg ggcaaacagg
    40981 atgggcggga aaaagttagc ggcgctggta aagggttgtt ccacatgcac cgggccgaac
    41041 gtcacaatac cgagttccac tctttttaag gccagcgaat cggcaagcag ctcatcgcga
    41101 aaggtaacca atccggcgtt aagttcatta atgggtctgc cgctcatcga gccagaaaca
    41161 tccagcagca aaatgcaggg gcaacgtggt tccgggttac tggcaaaatc gctggtggca
    41221 aacgtgattt gttcgctcat aagaaatcct ttcttagaga attattgagg tccggtatca
    41281 taccgactgc aataatgaaa taagagcaaa cgaagaaact ttcagaatta ggcccggcgg
    41341 cgcagaaaat tttgctattc gcctcgcaag aacggagagc cgtcgattga cactaagggc
    41401 ggagtgacat aatttcagga gtgagggtta gggagaggtt tccccctccc cctggtgttc
    41461 ttagtaagcc tggaagctaa tcactaagag tatcaccagt atgatgacgt gcttcatcat
    41521 aaccctttcc ttattaaaag ccctcttctc cgggagaggc tttcccgttt cagcgtcccg
    41581 ctgaaatcat cggcttacct cctttcgccc tgaatgcagt ctatcgctaa cgcgtgaata
    41641 aaatagtttc ctctgtgatt attgcgtaac gactcgcaaa gaatatttgc tggcaggatc
    41701 gcagactaca aagcctgcgg attgacaatc ttatcgtgaa ggcatacttt caggagtgag
    41761 ggtagagcgg ggtttccccc gccctggtag tcttagtaag cggggaagct tatgactaag
    41821 agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc cccttcctcg
    41881 ggaggggctt tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc tttcgccatg
    41941 caagcagtct atcgctaacg cgtagataaa atagtttcct gtgttattac tggatgcgtg
    42001 ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg tttcataatt cctccatttt
    42061 tctcccttat tggctggcta cactagtatc attccgcgaa acgtttcagg aagagaaact
    42121 cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc
    42181 cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg cagccccagg
    42241 ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc
    42301 attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcaccgg
    42361 gcttggcacc attatcgccg ctaatactgt tacggtgcgc agccgcgtgg acggccaact
    42421 gatagcgtta catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat
    42481 tgaccccagc cagttcaaag ttgcattagc acaagcccag ggccaactgg caaaagataa
    42541 agccacgctt gccaacgccc gccgtgacct ggcgcgttat caacaactgg caaaaaccaa
    42601 tctcgtttcc cgccaggaac tggatgccca acaggcgctg gtcagtgaaa ccgaaggcac
    42661 cattaaggct gatgaagcaa gcgtcgccag cgcgcagttg caactcgact ggagccgcat
    42721 caccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc
    42781 cagtggtgat accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt
    42841 taccctgccg gaaagcgata tcgctactgt agtgcaggcg caaaaagtcg gaaaaccgct
    42901 ggtggtagaa gcctgggatc gaaccaactc gaagaaatta agtgaaggca cgctgttaag
    42961 tctcgataac caaatcgatg ccactaccgg tacgattaaa gtgaaagcac gctttaataa
    43021 tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga
    43081 acaaaacgcc gtggtgatcc ccaccgccgc cctgcaaatg ggtaatgaag gccattttgt
    43141 ctgggtgctg aatagcgaaa acaaagtcag caaacatctg gtgacgccgg gcattcagga
    43201 cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg
    43261 cattgatcgc ctgaccgaag gggcgaaagt ggaagtagtg gaagcccaga gcgccactac
    43321 tccggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag
    43381 gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc
    43441 accacgctgc tgatggtggc gatcttactc gccgggatta tcggttatcg cgccctgccc
    43501 gtttcggcgc tgccggaagt ggactatccg accattcagg tggtcacgct ctacccaggt
    43561 gccagcccgg atgtcatgac ctctgccgtt accgcgccgc tagaacgcca gttcgggcag
    43621 atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg
    43681 cagttccagc taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac
    43741 gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac
    43801 ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacgcaa
    43861 gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggtgtcgga
    43921 ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg
    43981 attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt
    44041 aactcggcaa aaggtagcct cgacggccct tcccgtgcgg ttacgctttc cgctaacgac
    44101 cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca
    44161 attcgtctgg gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg
    44221 tgggcgaaca aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt
    44281 atctccaccg ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa
    44341 tcggtgaagg tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat
    44401 actcagtttg aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg
    44461 cgcaatattc cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact
    44521 ttcgcggtta tggtgtttct cgatttttca atcaataacc tgacactgat ggcgttaact
    44581 atcgccaccg gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat
    44641 atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt
    44701 accattatct cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc
    44761 gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc tggcggtagc gattttgatc
    44821 tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag
    44881 tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa aaatgttcga caggataatc
    44941 gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg
    45001 gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc
    45061 ccggtacagg acaatggcat tattcagggc actttgcagg caccgcaatc cagctccttt
    45121 gccaatatgg cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg
    45181 caaagcctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa cagtgcacat
    45241 ttacaaatca acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc
    45301 cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag
    45361 gatctgacta ttgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacg
    45421 tcactggatg cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca
    45481 cagctttctg atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt
    45541 gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa cgccctgtac
    45601 aacgcgtttg gtcagcggct gatttccact atttatactc aggccaacca gtatcgcgtg
    45661 gtgctggagc acaacaccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg
    45721 accagcagcg acggcggcgt ggtgccgcta agctcaattg ccaaaattga gcagcgtttt
    45781 gcgccgctct ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg
    45841 ccggataact attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg
    45901 aatctgccgg tggatatcac cacgcagttc cagggcagca ccctcgcctt ccagtcggcg
    45961 ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc
    46021 attctgtacg agagctttat tcacccgatc accattctct cgacgctacc caccgcagga
    46081 gtcggcgcac tgctggcgtt aatgattgcc ggtagcgaac tggatgtgat tgcgattatc
    46141 ggcattattt tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg
    46201 ctggctgctg agcgcgagca aggcgtgtcg ccgcgcgatg ccatctacca ggcttgtctg
    46261 ttgcgttttc gtccgatcct gatgaccact ctggcggctc tgcttggcgc gctgccgctg
    46321 atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc
    46381 ggcctgattg tcagccaggt gctgacgctg tttaccacgc cggtgattta tctgctgttc
    46441 gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc atgaagagga ggcgtaagtg
    46501 aagttttttg ccctcttcat ttaccgtccg gtggcgacga ttttactgtc ggttgccatt
    46561 accctgtgcg gcatactggg cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat
    46621 tttccggtga ttatggtcag cgcctcgctg cccggtgcgt caccagaaac aatggcgtct
    46681 tccgttgcca cgccgctgga gcgctcactt gggcgcattg ccggagtcag tgaaatgacc
    46741 tccagcagtt cgctcggcag cacgcgtatt attttgcagt ttgattttga ccgggatatc
    46801 aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc
    46861 gggatgccca gccgtccgac ctatcgcaaa gctaacccgt cggatgcgcc aattatgatc
    46921 ctcacgctga catccgatac ttattcgcag ggtgaactgt acgatttcac ctcgacgcag
    46981 ctggctccga cgatttcgca aatcgacggt gttggtgatg tcgatgtcgg cggcagctca
    47041 ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtctcgctg
    47101 gacgacgtac gcactgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa
    47161 gatggcactc accgctggca gatccagacc aatgatgagc taaaaaccgc cgccgaatat
    47221 cagccgctga ttattcatta caacaacggc ggcgcggttc gtctgggcga tgtggcgaca
    47281 gtgaccgact ctgtacagga tgtacgtaac gccgggatga ccaatgccaa accggctatt
    47341 ttgctgatga tccgcaagct gccggaagcc aatattatcc agacggttga cagcatccgg
    47401 gcaaaattac cggagttgca ggaaaccatt ccggcggcga ttgatctgca aattgctcag
    47461 gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatctcg
    47521 gtggcgctgg tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc aactattatt
    47581 cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcggt
    47641 ttcagtctca ataatctttc gttaatggcg ctcaccatcg ctactggttt cgtggtggat
    47701 gacgccatcg tggtgctgga aaacattgcc cgtcatctgg aagcgggaat gaaaccgttg
    47761 caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca
    47821 ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc
    47881 gaatttgccg tgacgctttc tgtcgccatt ggtatttcgt tgctggtttc tctgacatta
    47941 acgccaatga tgtgtggctg gatgctgaaa gccagcaagc cgcgcgagca aaagcgactg
    48001 cgtggttttg gtcgcatgtt ggtagccctg caacaaggct acggcaagtc actgaaatgg
    48061 gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc
    48121 tggctgtata tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg
    48181 ggcgggattc aggcggatca gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat
    48241 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt tacaggcggt
    48301 tcgcgagtga acagcgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
    48361 acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat
    48421 ctgttcctga tggcggtaca ggatattcgc gttggtgggc gtcagtcgaa cgccagctac
    48481 cagtacacgt tgttatccga cgacctggcg gcactgcgcg aatgggagcc gaaaatccgc
    48541 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
    48601 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacaa
    48661 gccgccaaca gtctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
    48721 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagt
    48781 gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag cgatcccgct gtcgtatttc
    48841 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
    48901 tcgaccattt cgtttaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
    48961 gatcgcgcaa tgacccagct tggggtgcct tcgacggtgc gcggcagttt tgccggcacg
    49021 gcgcaggtgt tccaggagac gatgaactcg caggtgatcc tgattatcgc cgccatcgcc
    49081 acggtgtata tcgtgctggg tatcctttac gagagttacg tacatccgct gacgattctc
    49141 tccaccctgc cctcggcggg cgttggagcg ctgttggcgc tggagctgtt caatgccccg
    49201 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
    49261 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
    49321 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgactac cctggcggcg
    49381 ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg gctcggagct gcggcaaccc
    49441 ctggggatca ccattgtcgg cggactggta atgagccagc tccttacgct gtataccacg
    49501 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
    49561 acggtaaccg agtaaatgac agatcttccc gacagcaccc gctggcaatt gtggattgtg
    49621 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
    49681 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
    49741 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
    49801 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
    49861 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg
    49921 gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
    49981 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
    50041 ctgctggtgg agtacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
    50101 atcggtgcga tcgccacatt gatgttaatg ccgaactata ccatgcagac gcggcgcttc
    50161 gatctctccg gctttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac
    50221 ggcagtaaag gtacaggttt atcgccgctg acgattgctg gcctggtcgc agttggcgtg
    50281 gtagcactgg tgctttattt gctgcacgcc agaaataaca accgcgctct tttcagtctg
    50341 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
    50401 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
    50461 ccgtttcatg ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga
    50521 attgttgtac aggtggtgaa ttgctttggt tatcgtcggg tactggtagc gaccacgctg
    50581 ggtctgtcgc tggtcaccct gttgtttatg actaccgctc tgctgggctg gtactacgtt
    50641 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac
    50701 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggaaacag cctgctgtcg
    50761 atgattatgc aattgtcgat gagtatcggc gtcaccatcg ccgggctgtt gctgggactt
    50821 tttggttcgc agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
    50881 tggcttagca tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac
    50941 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
    51001 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
    51061 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc
    51121 atggtaatga gcagcggctg caaatgctcg gcgatgccct gggcgagcag tatgcgcagc
    51181 atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat
    51241 ttgaacacga taattcggaa gataaacccg tcccgggtat gccaccgcac ggctggcgta
    51301 cccagttctg ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc
    51361 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg
    51421 cctcccccgt tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc
    51481 aaaccagctg gttgattgtc gctctggcaa cgttactcgc ggcgctcgcc acctttctgc
    51541 tggcgcgcgg tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
    51601 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc
    51661 aagacttcaa ccagcttgcc agcacactgg agaaaaacca gcaaatgcgg cgcgatttta
    51721 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
    51781 ccattcagga tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg
    51841 tcggtacact gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg
    51901 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
    51961 catttcgcga acgattcgcc agtcgtggcc tgaaactgca attttccctg ccagacagta
    52021 ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta ctggaaaaca
    52081 gcctgcgcta cactgacagc ggcggcagcc tgcaaatctc tgccgggcag cgcgacaaaa
    52141 cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag tgacgatcag ctacaaaaat
    52201 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg tgccagcggc ggttccgggc
    52261 tggggctggc gatttgcctg aacattgttg aagcacataa tggtcgcatt attgctgccc
    52321 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
    52381 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
    52441 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac
    52501 gcttatcagc cacggcgatc aggtactgcc gtatgtgcgc cagacaccac cggatctgat
    52561 cctgttagat ctgatgctcc ctggcaccga tggcctgacg ctgtgccggg aaattcgtcg
    52621 tttttctgac attccgatcg tgatggtgac ggcaaaaatc gaagagatcg atcgcctgct
    52681 ggggctggag attggcgcag atgattatat ctgtaagccg tacagcccac gggaagtggt
    52741 agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag cgcgaattgc agcaacagga
    52801 tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat
    52861 gcttgacctg acgcctgcgg aatttcgtct gctgaaaacg ctatctcatg aaccaggaaa
    52921 agtgttctcc cgcgagcatt tgctcaatca tctttatgac gactaccgcg tagtaaccga
    52981 ccgcaccatc gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga
    53041 acagtcattt atccgcgccg tttacggcgt cggttaccgc tgggaagccg acgcctgccg
    53101 catcgtttag ttttagcgac attattttgt tagccggaga tggcgttcag acccgatccg
    53161 gcaacattat cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    53221 agaaaatcgc tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacaca
    53281 atttcaatca aggagtcatt atggctggtt ggtttgaact cattaagagc agtgataatc
    53341 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    53401 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    53461 aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcta
    53521 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    53581 ttgcctccgt taaagtcaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    53641 gccgggcgtg ggttgttggc gtccggcttt atatccaaat aacgcttagt tattaataat
    53701 taagagtaac cgccgccatt aaaatagtgg gcactgacga gcactcgttg tgctttcttt
    53761 gtttaccatc ccatacttat cctcctgaac atgttcatgt gccaaaatat cttctaatac
    53821 tttcgtgacg ctatccggtt tatgacatac actgatgtaa gatttttact tacaggcggg
    53881 cgtaaaatgg ccagaacgat gacggttgat ctcggcgatg agttgcgtga atttatcgag
    53941 tccttaattg aatccggcga ctatcgcacc caaagcgaag tgatccgtga gtcactgcga
    54001 ttacttcgag aaaagcaagc agagtctcgc ctgcaagcgt tacgtgacat gctcgcagaa
    54061 gggttgagta gtggtgaagc tcagccgtgg gaaaaggacg cgttcttacg gaaggtgaaa
    54121 gcagggataa ggaaataatg tgcgtattat taaactcatg ccaaaagcca atgaggattt
    54181 agaaggcatc tggtattaca gctatcacca tttcggcgaa ccacaagccg acagatatgt
    54241 ggaacatctt tcagacgttc tccagattct gagtaacaat aatataggta cgcccagacc
    54301 tgaattaggc gaaggtattt ttgttcttcc ttttgaacgc cacgtcattt atttcctgca
    54361 atcaccaggg gaaattatcg tcattcgtat ccttaaccaa aaccaggacg ccaccagaca
    54421 tttacactgg agctaaaccc gccgattgcg ctacaatgcc cgcccttaaa gtgggggcac
    54481 tcccctaacc gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat
    54541 gtttaaaccg gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc
    54601 ttatggcgca gatgctgttt atgcgggcca gccgcgttat tccctgcgtg tgcgcaacaa
    54661 cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa
    54721 gttttatgtc gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga
    54781 cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat
    54841 tatgctggtg cgtgagcact tccctgaaat gccgatccac ctttcggtgc aggctaacgc
    54901 cgtgaactgg gcgacggtga aattctggca gcaaatgggc ctgacccgcg tgatcctctc
    54961 tcgcgagctg tcgctggaag agattgaaga gatccgcaat caggtgccgg atatggagat
    55021 cgagatcttc gttcacggcg cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg
    55081 ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta
    55141 caacgtccag gaagggaaag aagatgatgt tggcaacatc gtacacaagt acgagccgat
    55201 tccggtgcaa aatgttgagc cgacgctggg tatcggcgca ccaaccgaca aagtgtttat
    55261 gatcgaagag gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac
    55321 ttacatcatg aactcgaaag atctgcgcgc catcgcccat gtagaacgcc tgaccaaaat
    55381 gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct ttctactatt gtgcacgcac
    55441 cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag
    55501 cctgctggaa actctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg
    55561 tcatactcac gacgattatc agaactacga atacggttat tcagtttctg accgccagca
    55621 gtttgttggt gagtttaccg gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa
    55681 taaattctcc gttggcgaca gccttgagct gatgacgccg caaggcaaca ttaattttac
    55741 ccttgagcac atggaaaacg ccaaaggcga agctatgccg atagcaccag gcgatggtta
    55801 tactgtgtgg ctcccggtcc cgcaggatct tgagctcaat tacgcgctgc tgatgcgtaa
    55861 tttctccggg gaaaccacgc gtaatcccca cggtaagtga ttaatttcga ttatttttcc
    55921 cggatggaaa attcttagaa accgatcaca tacagctgca tttattaagg ttatcatccg
    55981 tttcgctgaa aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct
    56041 gtgtaatctc ccttacacgg gcttattttt tatgcataag ccctatccct ggtcaccgtc
    56101 ttccattgac cacatcgata gaatctccct tcatagcacg atgcctttca cgtaacggca
    56161 tcgtgctcgc acaggttccg gctaagcaca accagaacgc gcatgtttga cgcttaccaa
    56221 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    56281 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    56341 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    56401 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    56461 tttaccgata cttgtctcat cattatgcac agcacaacca gacacaataa aaatactaat
    56521 taatgatata gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc
    56581 tctagtgttt aaatataata atgg
//
LOCUS       DAZFVO020000008        55159 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFVO020000008 DAZFVO020000000
VERSION     DAZFVO020000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 55159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.008"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..715)
                     /locus_tag="ACHKNB_RS02150"
                     /old_locus_tag="pgaptmp_000430"
     CDS             complement(<1..715)
                     /locus_tag="ACHKNB_RS02150"
                     /old_locus_tag="pgaptmp_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HGU4194009.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVL"
     gene            complement(758..1234)
                     /locus_tag="ACHKNB_RS02155"
                     /old_locus_tag="pgaptmp_000431"
     CDS             complement(758..1234)
                     /locus_tag="ACHKNB_RS02155"
                     /old_locus_tag="pgaptmp_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HGU4194010.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPR"
     gene            complement(1255..3336)
                     /locus_tag="ACHKNB_RS02160"
                     /old_locus_tag="pgaptmp_000432"
     CDS             complement(1255..3336)
                     /locus_tag="ACHKNB_RS02160"
                     /old_locus_tag="pgaptmp_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HGU4194011.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(3333..4874)
                     /locus_tag="ACHKNB_RS02165"
                     /old_locus_tag="pgaptmp_000433"
     CDS             complement(3333..4874)
                     /locus_tag="ACHKNB_RS02165"
                     /old_locus_tag="pgaptmp_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4194012.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLSMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(4884..5660)
                     /locus_tag="ACHKNB_RS02170"
                     /old_locus_tag="pgaptmp_000434"
     CDS             complement(4884..5660)
                     /locus_tag="ACHKNB_RS02170"
                     /old_locus_tag="pgaptmp_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HGU4194013.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(5670..6512)
                     /locus_tag="ACHKNB_RS02175"
                     /old_locus_tag="pgaptmp_000435"
     CDS             complement(5670..6512)
                     /locus_tag="ACHKNB_RS02175"
                     /old_locus_tag="pgaptmp_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4194014.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(6522..7313)
                     /locus_tag="ACHKNB_RS02180"
                     /old_locus_tag="pgaptmp_000436"
     CDS             complement(6522..7313)
                     /locus_tag="ACHKNB_RS02180"
                     /old_locus_tag="pgaptmp_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HGU4194015.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            7526..8173
                     /locus_tag="ACHKNB_RS02185"
                     /old_locus_tag="pgaptmp_000437"
     CDS             7526..8173
                     /locus_tag="ACHKNB_RS02185"
                     /old_locus_tag="pgaptmp_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4194016.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(8157..8795)
                     /gene="ytfB"
                     /locus_tag="ACHKNB_RS02190"
                     /old_locus_tag="pgaptmp_000438"
     CDS             complement(8157..8795)
                     /gene="ytfB"
                     /locus_tag="ACHKNB_RS02190"
                     /old_locus_tag="pgaptmp_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HGU4194017.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            9014..9634
                     /gene="fklB"
                     /locus_tag="ACHKNB_RS02195"
                     /old_locus_tag="pgaptmp_000439"
     CDS             9014..9634
                     /gene="fklB"
                     /locus_tag="ACHKNB_RS02195"
                     /old_locus_tag="pgaptmp_000439"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4194018.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            9943..11355
                     /gene="cycA"
                     /locus_tag="ACHKNB_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /gene_synonym="dagA"
     CDS             9943..11355
                     /gene="cycA"
                     /locus_tag="ACHKNB_RS02200"
                     /old_locus_tag="pgaptmp_000440"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HGU4194019.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(11400..12062)
                     /gene="ytfE"
                     /locus_tag="ACHKNB_RS02205"
                     /old_locus_tag="pgaptmp_000441"
     CDS             complement(11400..12062)
                     /gene="ytfE"
                     /locus_tag="ACHKNB_RS02205"
                     /old_locus_tag="pgaptmp_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HGU4194020.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(12170..13135)
                     /gene="ytfF"
                     /locus_tag="ACHKNB_RS02210"
                     /old_locus_tag="pgaptmp_000442"
     CDS             complement(12170..13135)
                     /gene="ytfF"
                     /locus_tag="ACHKNB_RS02210"
                     /old_locus_tag="pgaptmp_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4194021.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKYPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(13243..14103)
                     /gene="qorB"
                     /locus_tag="ACHKNB_RS02215"
                     /old_locus_tag="pgaptmp_000443"
     CDS             complement(13243..14103)
                     /gene="qorB"
                     /locus_tag="ACHKNB_RS02215"
                     /old_locus_tag="pgaptmp_000443"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4194022.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            14192..14572
                     /gene="ytfH"
                     /locus_tag="ACHKNB_RS02220"
                     /old_locus_tag="pgaptmp_000444"
     CDS             14192..14572
                     /gene="ytfH"
                     /locus_tag="ACHKNB_RS02220"
                     /old_locus_tag="pgaptmp_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HGU4194023.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(14701..16644)
                     /gene="cpdB"
                     /locus_tag="ACHKNB_RS02225"
                     /old_locus_tag="pgaptmp_000445"
     CDS             complement(14701..16644)
                     /gene="cpdB"
                     /locus_tag="ACHKNB_RS02225"
                     /old_locus_tag="pgaptmp_000445"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HGU4194024.1"
                     /translation="MIKFSATLLATLITASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLTAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            16834..17574
                     /gene="cysQ"
                     /locus_tag="ACHKNB_RS02230"
                     /old_locus_tag="pgaptmp_000446"
     CDS             16834..17574
                     /gene="cysQ"
                     /locus_tag="ACHKNB_RS02230"
                     /old_locus_tag="pgaptmp_000446"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HGU4194025.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(17564..18121)
                     /gene="ytfJ"
                     /locus_tag="ACHKNB_RS02235"
                     /old_locus_tag="pgaptmp_000447"
     CDS             complement(17564..18121)
                     /gene="ytfJ"
                     /locus_tag="ACHKNB_RS02235"
                     /old_locus_tag="pgaptmp_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4194026.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLNKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            18446..18652
                     /gene="ytfK"
                     /locus_tag="ACHKNB_RS02240"
                     /old_locus_tag="pgaptmp_000448"
     CDS             18446..18652
                     /gene="ytfK"
                     /locus_tag="ACHKNB_RS02240"
                     /old_locus_tag="pgaptmp_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HGU4194027.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(18714..20057)
                     /gene="paeA"
                     /locus_tag="ACHKNB_RS02245"
                     /old_locus_tag="pgaptmp_000449"
     CDS             complement(18714..20057)
                     /gene="paeA"
                     /locus_tag="ACHKNB_RS02245"
                     /old_locus_tag="pgaptmp_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGU4194028.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(20380..21018)
                     /gene="msrA"
                     /locus_tag="ACHKNB_RS02250"
                     /old_locus_tag="pgaptmp_000450"
     CDS             complement(20380..21018)
                     /gene="msrA"
                     /locus_tag="ACHKNB_RS02250"
                     /old_locus_tag="pgaptmp_000450"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HGU4194029.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDQGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            21224..22957
                     /gene="tamA"
                     /locus_tag="ACHKNB_RS02255"
                     /old_locus_tag="pgaptmp_000451"
     CDS             21224..22957
                     /gene="tamA"
                     /locus_tag="ACHKNB_RS02255"
                     /old_locus_tag="pgaptmp_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HGU4194030.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            22954..26733
                     /gene="tamB"
                     /locus_tag="ACHKNB_RS02260"
                     /old_locus_tag="pgaptmp_000452"
     CDS             22954..26733
                     /gene="tamB"
                     /locus_tag="ACHKNB_RS02260"
                     /old_locus_tag="pgaptmp_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HGU4194031.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDDKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLENDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            26736..27077
                     /gene="ytfP"
                     /locus_tag="ACHKNB_RS02265"
                     /old_locus_tag="pgaptmp_000453"
     CDS             26736..27077
                     /gene="ytfP"
                     /locus_tag="ACHKNB_RS02265"
                     /old_locus_tag="pgaptmp_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HGU4194032.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            27289..27540
                     /gene="chpS"
                     /locus_tag="ACHKNB_RS02270"
                     /old_locus_tag="pgaptmp_000454"
     CDS             27289..27540
                     /gene="chpS"
                     /locus_tag="ACHKNB_RS02270"
                     /old_locus_tag="pgaptmp_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HGU4194033.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            27534..27884
                     /gene="chpB"
                     /locus_tag="ACHKNB_RS02275"
                     /old_locus_tag="pgaptmp_000455"
     CDS             27534..27884
                     /gene="chpB"
                     /locus_tag="ACHKNB_RS02275"
                     /old_locus_tag="pgaptmp_000455"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HGU4194034.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(27964..28494)
                     /gene="ppa"
                     /locus_tag="ACHKNB_RS02280"
                     /old_locus_tag="pgaptmp_000456"
     CDS             complement(27964..28494)
                     /gene="ppa"
                     /locus_tag="ACHKNB_RS02280"
                     /old_locus_tag="pgaptmp_000456"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HGU4194035.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            28804..29760
                     /gene="ytfQ"
                     /locus_tag="ACHKNB_RS02285"
                     /old_locus_tag="pgaptmp_000457"
     CDS             28804..29760
                     /gene="ytfQ"
                     /locus_tag="ACHKNB_RS02285"
                     /old_locus_tag="pgaptmp_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HGU4194036.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            29900..31402
                     /gene="ytfR"
                     /locus_tag="ACHKNB_RS02290"
                     /old_locus_tag="pgaptmp_000458"
     CDS             29900..31402
                     /gene="ytfR"
                     /locus_tag="ACHKNB_RS02290"
                     /old_locus_tag="pgaptmp_000458"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HGU4194037.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            31416..32438
                     /gene="ytfT"
                     /locus_tag="ACHKNB_RS02295"
                     /old_locus_tag="pgaptmp_000459"
     CDS             31416..32438
                     /gene="ytfT"
                     /locus_tag="ACHKNB_RS02295"
                     /old_locus_tag="pgaptmp_000459"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HGU4194038.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            32425..33420
                     /gene="yjfF"
                     /locus_tag="ACHKNB_RS02300"
                     /old_locus_tag="pgaptmp_000460"
     CDS             32425..33420
                     /gene="yjfF"
                     /locus_tag="ACHKNB_RS02300"
                     /old_locus_tag="pgaptmp_000460"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HGU4194039.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(33453..34451)
                     /gene="fbp"
                     /locus_tag="ACHKNB_RS02305"
                     /old_locus_tag="pgaptmp_000461"
     CDS             complement(33453..34451)
                     /gene="fbp"
                     /locus_tag="ACHKNB_RS02305"
                     /old_locus_tag="pgaptmp_000461"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HGU4194040.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            34627..36000
                     /gene="mpl"
                     /locus_tag="ACHKNB_RS02310"
                     /old_locus_tag="pgaptmp_000462"
     CDS             34627..36000
                     /gene="mpl"
                     /locus_tag="ACHKNB_RS02310"
                     /old_locus_tag="pgaptmp_000462"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HGU4194041.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGHGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPTHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(36156..36707)
                     /gene="yjgA"
                     /locus_tag="ACHKNB_RS02315"
                     /old_locus_tag="pgaptmp_000463"
     CDS             complement(36156..36707)
                     /gene="yjgA"
                     /locus_tag="ACHKNB_RS02315"
                     /old_locus_tag="pgaptmp_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HGU4194042.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            36801..38153
                     /gene="pmbA"
                     /locus_tag="ACHKNB_RS02320"
                     /old_locus_tag="pgaptmp_000464"
     CDS             36801..38153
                     /gene="pmbA"
                     /locus_tag="ACHKNB_RS02320"
                     /old_locus_tag="pgaptmp_000464"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HGU4194043.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            38336..38722
                     /gene="cybC"
                     /locus_tag="ACHKNB_RS02325"
                     /old_locus_tag="pgaptmp_000465"
     CDS             38336..38722
                     /gene="cybC"
                     /locus_tag="ACHKNB_RS02325"
                     /old_locus_tag="pgaptmp_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4194044.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(38767..39231)
                     /gene="nrdG"
                     /locus_tag="ACHKNB_RS02330"
                     /old_locus_tag="pgaptmp_000466"
     CDS             complement(38767..39231)
                     /gene="nrdG"
                     /locus_tag="ACHKNB_RS02330"
                     /old_locus_tag="pgaptmp_000466"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HGU4194045.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(39389..41527)
                     /gene="nrdD"
                     /locus_tag="ACHKNB_RS02335"
                     /old_locus_tag="pgaptmp_000467"
     CDS             complement(39389..41527)
                     /gene="nrdD"
                     /locus_tag="ACHKNB_RS02335"
                     /old_locus_tag="pgaptmp_000467"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGU4194046.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(41921..43576)
                     /gene="treC"
                     /locus_tag="ACHKNB_RS02340"
                     /old_locus_tag="pgaptmp_000468"
     CDS             complement(41921..43576)
                     /gene="treC"
                     /locus_tag="ACHKNB_RS02340"
                     /old_locus_tag="pgaptmp_000468"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGU4194047.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(43610..45031)
                     /gene="treB"
                     /locus_tag="ACHKNB_RS02345"
                     /old_locus_tag="pgaptmp_000469"
     CDS             complement(43610..45031)
                     /gene="treB"
                     /locus_tag="ACHKNB_RS02345"
                     /old_locus_tag="pgaptmp_000469"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HGU4194048.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(45150..46097)
                     /gene="treR"
                     /locus_tag="ACHKNB_RS02350"
                     /old_locus_tag="pgaptmp_000470"
     CDS             complement(45150..46097)
                     /gene="treR"
                     /locus_tag="ACHKNB_RS02350"
                     /old_locus_tag="pgaptmp_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HGU4194049.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            46282..46335
                     /gene="mgtL"
                     /locus_tag="ACHKNB_RS02355"
                     /old_locus_tag="pgaptmp_000471"
     CDS             46282..46335
                     /gene="mgtL"
                     /locus_tag="ACHKNB_RS02355"
                     /old_locus_tag="pgaptmp_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HGU4194050.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            46476..49172
                     /gene="mgtA"
                     /locus_tag="ACHKNB_RS02360"
                     /old_locus_tag="pgaptmp_000472"
     CDS             46476..49172
                     /gene="mgtA"
                     /locus_tag="ACHKNB_RS02360"
                     /old_locus_tag="pgaptmp_000472"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HGU4194051.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(49378..49764)
                     /gene="ridA"
                     /locus_tag="ACHKNB_RS02365"
                     /old_locus_tag="pgaptmp_000473"
     CDS             complement(49378..49764)
                     /gene="ridA"
                     /locus_tag="ACHKNB_RS02365"
                     /old_locus_tag="pgaptmp_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HGU4194052.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(49837..50298)
                     /gene="pyrI"
                     /locus_tag="ACHKNB_RS02370"
                     /old_locus_tag="pgaptmp_000474"
     CDS             complement(49837..50298)
                     /gene="pyrI"
                     /locus_tag="ACHKNB_RS02370"
                     /old_locus_tag="pgaptmp_000474"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HGU4194053.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(50311..51246)
                     /gene="pyrB"
                     /locus_tag="ACHKNB_RS02375"
                     /old_locus_tag="pgaptmp_000475"
     CDS             complement(50311..51246)
                     /gene="pyrB"
                     /locus_tag="ACHKNB_RS02375"
                     /old_locus_tag="pgaptmp_000475"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HGU4194054.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWRLHSSIEE
                     VIAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(51250..51384)
                     /gene="pyrL"
                     /locus_tag="ACHKNB_RS02380"
                     /old_locus_tag="pgaptmp_000476"
     CDS             complement(51250..51384)
                     /gene="pyrL"
                     /locus_tag="ACHKNB_RS02380"
                     /old_locus_tag="pgaptmp_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HGU4194055.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(51665..52060)
                     /gene="yjgH"
                     /locus_tag="ACHKNB_RS02385"
                     /old_locus_tag="pgaptmp_000477"
     CDS             complement(51665..52060)
                     /gene="yjgH"
                     /locus_tag="ACHKNB_RS02385"
                     /old_locus_tag="pgaptmp_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4194056.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(52191..52904)
                     /gene="bdcA"
                     /locus_tag="ACHKNB_RS02390"
                     /old_locus_tag="pgaptmp_000478"
     CDS             complement(52191..52904)
                     /gene="bdcA"
                     /locus_tag="ACHKNB_RS02390"
                     /old_locus_tag="pgaptmp_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4194057.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            52975..53568
                     /gene="bdcR"
                     /locus_tag="ACHKNB_RS02395"
                     /old_locus_tag="pgaptmp_000479"
     CDS             52975..53568
                     /gene="bdcR"
                     /locus_tag="ACHKNB_RS02395"
                     /old_locus_tag="pgaptmp_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGU4194058.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILLDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            53713..54165
                     /gene="tabA"
                     /locus_tag="ACHKNB_RS02400"
                     /old_locus_tag="pgaptmp_000480"
     CDS             53713..54165
                     /gene="tabA"
                     /locus_tag="ACHKNB_RS02400"
                     /old_locus_tag="pgaptmp_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HGU4194059.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            54287..>55159
                     /locus_tag="ACHKNB_RS02405"
                     /old_locus_tag="pgaptmp_000481"
     CDS             54287..>55159
                     /locus_tag="ACHKNB_RS02405"
                     /old_locus_tag="pgaptmp_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4194060.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHNKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKYSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILID"
BASE COUNT        13293 a        13870 c        14537 g        13459 t
ORIGIN      
        1 ttagtacgcc acccataatt aacacaatca ggaaaatact ggggatagcc atccatatcg
       61 caacgccagc ctggcgcaaa gtcggacgtt ctggttttgg ataaccgcga cgtacagcga
      121 taataccggc tattaccatc agtgccagac ccagcaaaat ccccggaaca taacccgcca
      181 ggaacagtgc ggcaatagaa gtaccaccac tgaccagtga atagacgatt aacgtattac
      241 ttggtggaat aagcaaaccc gtcggggcag aggcaacgtt aaccgcagta ctgaatgccg
      301 ggtcgtagcc ctctttaacc tgttgcggat gcatgacgcc gcccatcgca gccgccgaag
      361 ccacggctga accagaaatt gccccgaaca gcatattggc gagaatatta cagtgggcaa
      421 gtgagcctgg cagacgaccg ccgagaatac gggcaaagtt gattaatcgt cgcgctattc
      481 ctccatgatt cataatgttg ccagacaaga taaaaaatgg gattgccaac aacgtaaaac
      541 tatctaaccc ggaggctaac ttttgggaaa ccacactcaa tgctgagttg aaatcgagtt
      601 gtgtggaaat tgatgccagt gtcgataaac caatggcata aacaataggt acacctaatg
      661 ccaacataaa aaagaaggca ccaaataaca caataatcgg taaccagtaa tccataattt
      721 ctccattcct actatggttg gttgttgttt gttatcttta gcgtggttgt ttaagttcgg
      781 ttaaaaccat atctatggtg tatataataa taagtacacc actaatcggg atgctcatat
      841 agatatatcc catttgccag cctaaagcgg gcgaggtctg acctgaatcg aatgtatcca
      901 tcgttaatag cccaccacca tataccataa tgatggttgc gaagataata acgataatct
      961 gaatacacaa aaataaatga cgtcgggctt ttcctttcag catcataggt agcaggtcaa
     1021 tcgccagatg ccggttttgc cctgcggtgt atgccccacc aaaaagtcca agacaaataa
     1081 agagcaaacg gctgagttca tctgtaaaaa tactggggct agtaaatata aatcgtgcag
     1141 ccacctgcca cgtcacacat gccacgatga tcgccaaaat ggtaatgttc aatgctgcaa
     1201 gcagagcatt aatagatcta attatatttc gcatagtgat taacctttgc aatatcattc
     1261 atcctgtggc tggtaaattg tcccattaag aaaaccaggc cagttttttt caaaccaaat
     1321 acctcggata ataggaaact cacgaacagg tgtttctctg acgtgaaaga taccgttgca
     1381 ataatgaata agtagtttat cttttttatc ctcagtacgc tctgaacgaa agcgccaggc
     1441 atcacaaaaa ccatttcgtg tttgcggaat agaatttcca tccctgtcga gaataatacg
     1501 tgctccccga ctaccaccac ctgcgtcaat ataatgcgtt aattgcgtta atacggcggc
     1561 agaagtcagt gccatatgcc gccacataaa cagctcggca acttcaccca catgtttgat
     1621 agccaatcca tgccgttgga caaattcgct caatagcagg gcatcgcgag tggcgcgtcg
     1681 gactttatcg gcatggcaaa taaatcccgc atggtcagac attcgtgcct gaattttttc
     1741 tctcacaacc gacaacggca tcccggtccc gttatcgtgc gcctgagtaa tgatctctct
     1801 tatagaagcc agtgtaggag ctaccagctg tgctatatct ccatcaatat tccgcttttg
     1861 cgtgcaacca ataaaacgtg ccagacgaac agcaaaaacc tgcccggcat tcaatgctgc
     1921 accaccaggg cgagtgacgc cgtgtgtgcc agcaacttcc cccacggcga aacagccggg
     1981 cagggaggtt tgtccccaga tatccacttc aatgccgcca ttcatatgct gattattcat
     2041 ggcaaactgc aaaggctgtg tggtgagatc gtaaccgtgc atcttataca gcgagataga
     2101 cagcggattc attcgttgca gccgttcgat gggcgatggt gccagagcgt cgttattttc
     2161 cagatacgcg cgaacgtcgt cgtccagtcg atctaatgag aacggcaggt cacccggcac
     2221 cgcttcagga ttgcgattga aatccataaa tacctgacgc cctgattgct gctcttgcgc
     2281 tactgccata tctaacaagc tggagccaaa atccatcacc cgcgtggcgt ggaacggcca
     2341 ctgatagcct ttacggaaaa tgtttgaagc cagctcctgg gtggtgcgat agtaatccgc
     2401 gaggaagtta tactcgttac cctcagcatc cacggaatag atatacggga tcacctgtac
     2461 ataggtgccg gataaattcc acggaaacgt gctacgcggc gtgcctatac caaactggct
     2521 ttcggtcaga ttggttagcg tcagaccttc ctccagcgcc agccccagcg agccgaagca
     2581 tttatgtgga taaacactgt cgcgataaag ctcgccaggc cctcctgttg ccagtaccac
     2641 attgggcgca gtcacaattg ccagccccca tgggttatgg gcgcgatgac cggtcgcgag
     2701 gattgcccct gccacgcggt cttcgccgtt ttcgtcacgc tgatgcagca gtttaatcac
     2761 tgttgcactg gtcaacactg gaatggcgag gcgctgtact tcttccaaca gcactttcac
     2821 cattaacctt gaggtacgcg gcccacacga ggttgcacgc ccggcttcgt catggtcggt
     2881 ttgataacga agaatcgcgc catagcgatc ttccggtaat tccagaccaa gatattgcaa
     2941 cccgcctaga gtgtgcagag aacctaccgc ttcgacataa gcggtgtcgt gatccatcgc
     3001 cccaccgctc gccagtgcct ctgccagttt ggtgaagttg tcgccgttgc ccgcggtagc
     3061 ggcggtaaat agtgtctgtt tatcggaacc agaacatgcc gacgtcccca tatataaccc
     3121 ggcggtagcg atcagcacat tttgctggcg gcgtttaagt tcaactgccg cacgtaatcc
     3181 tgctgcacca ctgccgacta ccagggcatc tgtttgccag agtgggtaat caacaccgtc
     3241 taccgtgaca acccatgcgg gctctgcctg tgcaaaaccc tttgggaaag taacgtgcgt
     3301 cagcgcatca aggatggtgt catcaagctg actcatgcat cctccatcgg cgtaaacagg
     3361 ctactttcca tcaggcgtgg gtgcttgata attggacgga aagccatata cggcagaatg
     3421 tcgcgctcaa tctccacacc cggcgcgatt tcaattaatt caacgccttc tgccgttaac
     3481 tggaatactg cgcgttcagt gatatacaac acttcctgac cactttgctg tgcgtaggcg
     3541 gcgctaaaag tgattttttc cacctgggta accagtttag ggatttgacc tgactgggtg
     3601 atatgcaagc catctggcgt tacatcaatc ttgctacctt tggcgtcgaa cgtgccgcag
     3661 aacaccactt tacgggcgtt ttgcgcaatt tcgagaaatc cgccggggcc aatcagattg
     3721 ccattgagat gggagacgtt gacgttaccg tactgatcca tctctcccat gctgaggaag
     3781 gtgatatcga tccctccgcc actgtagaac tcaaattgat cgagtgatgg aataatggca
     3841 tcggcgttgc gggcgcagcc gaaaaacgca tcatccagca tcataccgtt atgcacgcct
     3901 tgctcgacgc tcagccataa actttggtaa ggcacgcctt cgcgcagcgc tacgcctgga
     3961 atacccccag gaataccaaa gccaaagttg gttgaagcgc cagctaccag ttcgcgtgcc
     4021 gcccgacggg caatcaggcg acgtaccgga tgactaacca gttcaatggc gtcgttagaa
     4081 ctcagacggc gatgttggcc gctgatggtc aggtcgtaac cgccaagata ggtttgtggt
     4141 tgttcgcggt gttcaacgat gccatcaacc agaatgcccg gtaatttgac ccggcgtggt
     4201 tcaatcgcgc cagcttccag cacatcgcgt acctgtacga acactttgcc gccgctgttg
     4261 tgtgctgcca gcgccatgga atagctatcc atatcaatcg cttcttcttc aaggctgacg
     4321 ttgcctcgag gatcggcata agtgccacgc aaaatcgcgt aatccacttt gaaagggcga
     4381 taacgaagtt tggtttcacc gtcgatttca atcagttcta ccaggtcgtc ggtagtgcat
     4441 tcattcgact taccgccgcc attgcgtgga tcaacaaacg atcccagccc aacgtgggta
     4501 aaaagccccg gacgtccggc gccgatctcc cgtagcagtg cctgaatcac gccaccagga
     4561 aagcagtagg cttcaatcgt gttatttttt accagcgcct gcattttggg cgaccaggta
     4621 aagtgcccgg caataatgcg tttgagcatc tctgcgtgcg caaaacggtt agtgcctttg
     4681 cagtcgcgat cgccaatacc cagcgagtgg attagcgtca ggtcgcgcgg gtgtccggtt
     4741 tgcaggaaac gcgcttcaat ggcggccata atatggtcag cttccaccat gccgccgccg
     4801 ttaccgctaa tggcaatggt tgcgccgtcc tgaatttgcg ccgccagtgc ctcggcggtt
     4861 gttatcttac gcatacaatt tccttattga ttatggaagc tcacgctact tttttcggca
     4921 aaggcgcgcg tcgcctgctg gcaatcctga gtggcagaca ttaatccggc cagtagctgg
     4981 tgcgggtaat cggcatgttt attttccagt gccagaatca gttgcttaat atgcgccagt
     5041 gccaccggac cgcatttttc cagctgcgct aactggttcg ccatccagct ttccacctgt
     5101 tctttttcta ccacttcatc aattaaattc gccgcctggg cttcctgcgc cgtcaggcgt
     5161 ttacccagca tcagcatctg gcgaccgacc accggaccaa cctgattaag cacacgttca
     5221 atgcccatcc agccaggcac catgccgagc atcacttccg ggttagagaa tttcgcgccg
     5281 gggcgggcaa tacgaatatc gcagcacaag gccagttcaa taccaccgcc gatggtatga
     5341 ccgttgagat tcgcaacggt taattggggt atgtttcgca ggcgattaaa aacgtcatta
     5401 ccgcgtttaa tccattttct gcccatatct aatggcgaat aggccgacca cgatttaata
     5461 tcgccgcccg cacaaaagaa tttatcgccg gttgccgtta attcgactaa tcgtaatgtg
     5521 gtgtcgtttt caatttcatt taaataatgg tcgagagatt cgatcatctc ttcgttaata
     5581 gcatgacatt tatcttcacg gttcaatgtc aggcggcagg aagccgccac tctgctgaac
     5641 agaacgggtt ggtcactcat gatcattcct taacaataat gcgcgatgcg atcgacgcta
     5701 atatccagcg tgctgttaat atctttctgc caccagtaag gcgagaatat ttccagttca
     5761 atagcaccat taaatccggc gttttcaacc agccgacgaa ttgacggaat attgatgacg
     5821 ccatctcccg gcataccacg gtcattgacc agatcagtgg tcggcactaa ccagtcggaa
     5881 acatgaaacg cgaggatgcg ttttccggca cgcaggatct gactggcgag atccggatcc
     5941 caccagacgt gataaacatc caccgccacg ccgaggccaa actcaccatc gggatcgagt
     6001 tcatcacacc agtccagcgc ctggcgtaac gtgcacaggc aagagcgatc ggcagctgtc
     6061 atcgggtgca gtggctccag cgcaattggc acgccgacat ctttcgaatg tggcagcaac
     6121 tggcgaatcc cttgttttac ttgttcccgc gcttcgtaca gatcttttgt gcccgttggc
     6181 agaccgccaa ccacctgcat ataacaggcg gcgttaagca ccgctgcgtc gtccagcgcc
     6241 cggcggttat catcaatcgc cagtttgcgt tccgccagtg tcggcgcggt gtagtaagtg
     6301 ctacggcaca aaccggaaac gctcatgtta ctggcggcaa gctggtgggc gatttgctgg
     6361 agatcttcac cttgcaactc cctgcgccag ggagcaatcg cgccaatacc acgcgcggcg
     6421 caggcatcaa taatggcggg tagcggcgtt ttatagccca gtgttgcggt attgatactg
     6481 aataaatccg ggcgttgtaa cagatctctc atgattcagc ctcagaagcg cggcatactt
     6541 aagccgccgt cgatggcgac cgcctgacca caggtgtaaa tcagtttacc ttccgccatc
     6601 gcgcggacag tggaagcaat atcagccgga taaccccaac gtccccacgg caccagacct
     6661 ttggcaatca gttcgtcgta ataagccgtt gccggaatag tcatatcggt tttgatgaga
     6721 ccagggcgca cttcataaac gccaatttgt tcattacaaa ggcgagcagc aaacagacga
     6781 gcagcggcag aaacggcggt tttggcaatg gtgtattcgc cgcgattcat cgccagcatg
     6841 atggcgttga tggaactgac gaaaataatc gagcggtgcg gcagctccgc ttccggtttc
     6901 ggctgtgcca gtaagcgctt actgaatgcc tgagcaagga agaacggcgc gcgggtgtta
     6961 atggcaatgt tttgatcgaa gctgtctggt tcgaggtcga ggaggtcacc gcgttttttc
     7021 actgaaatcc ccgcgttatt gagcagacag tccagccgcc cccactggtt ttgcgccgcg
     7081 tcgagcatct cttcatgcaa ggagagatcg ccgacatcgg cagggaagca aatgacttcc
     7141 acgccttctg ccaaacattc ttgttgtgtg atatgcagtt tttctacgct gtcggcatcg
     7201 ggacgatcgt taatcagcag gttaaatccg ccacgagcaa gctccagcgc acagcccttt
     7261 ccaataccac gagcggctcc ggtaatcacg gcaacaggac gagtggattt catacaatag
     7321 ctctcttttt ttgaatattg ttttctaagt gacaattagc ttgcgacgtg ccgcaagaaa
     7381 ggtcaaccat ctggttggtt gatatgcgat agtgatcaca gtacatttga taacttgatg
     7441 aaagtacaag tttttatcct ttttactgga gcttctaatt aatcgggtta agatagttat
     7501 cttgaagagg tcgtggggaa gcgttatgta tattgatacc agcaacgtac agagcctgaa
     7561 ggaaaaattg ctgctatgtg cggttaatga atttgccgaa tatggttatg aaggggccag
     7621 agtagataat attgtaaagg cagcagggtg tagtaaacag acggtttatc accactttgg
     7681 taataaagaa aatttattta tcgaagtgct ggaatatacc tggaacgata ttcgacaaaa
     7741 agaaaaggcc cttgatttct cagatcttcc gccacaaaaa gctattgaga aaattattga
     7801 ttttacatgg gattattata ttgctaaccc ctggttttta aaaattgtgc atagtgaaaa
     7861 tcaaagtaaa ggtgtgcatt atgcaaaatc acagcgtctc ttagaaatta accatgcgca
     7921 cttgcagtta atggaatcct tattggatga agggaagaaa cacaatattt ttaagccaga
     7981 tatcgaccca ttgcaggtga atattaatat tgctgcgctt gggggatatt atttgatcaa
     8041 ccagcatacg cttggcctgg tttatcatat cagtatggtt tccccccagg cactggaagc
     8101 cagacgtaag gtcatcaaag agacaatcct tagctggctt ttggttgacc cttcatctac
     8161 cgcgcacgaa taaaactgcc atccggctgg cgggtaaaca ggacctgttg attattcccc
     8221 gtatcaatgg ttaagcccgt caccacgccg ctggcgttct ggcggatttt caccatttgc
     8281 ccgttttgca aattactcag cggcttaccc gcgccttcta cctgcgccat tgcatagaca
     8341 tcggtagcgg gcagtccgtg gtcgcggaac agctgcgcca tcgttttacc gggttcgaca
     8401 cggtaagagc gccattgatt atcaattccg ctatctggct ggaatggttg cgtttgggta
     8461 gtttgcggct gctcttctgg ctggccttcc tgtataggtt ctggagcgac cggagccacc
     8521 tgatcaggat cattttgtgg ggtgactaat tgcgcccgca gctgttcttc tgtcggcggt
     8581 tgtgattgcg actgaatatc taactgcgct tcacgcgtca ccaccggcgc gttgggcgta
     8641 tcatcagatg gaagcagaaa tccgaccacc aatacaatgg cggcaataat gatcccccga
     8701 cgatgcatcg gcggcagcgg gtccatgaag cgaaaattgt ccggcgcgtg ccagactttc
     8761 tccagggttg gttttagttc aaagcgcccg ggcatggttt cctcctgctc cgcgtcttgt
     8821 tcctcaatca tagcctatga ataagctaac gctatgatgt ccgtggtaaa cccgccttta
     8881 ttatattcat acgggatatt gctattgttt ctttttcccc gggatttgtc atcattcctg
     8941 cgacaaagtt ttacccaaag aagtgtggct gatatgctgc ccgctacttt tataccctaa
     9001 gaaaggaaat acgatgacca ccccaacttt tgacaccatc gaagcgcaag caagctacgg
     9061 cattggtttg caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga
     9121 agcactggtt gcaggtattg ccgatgcgct ggaaggcaaa catccggctg ttccggttga
     9181 tgtggtgcat cgcgcgctgc gtgaaatcca cgagcgcgcc gatgccgttc gtcgtcagcg
     9241 tttccaggcg atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg
     9301 tgtgaatagc accgaatctg gcctgcaatt ccgcgtgatc aaccagggtg aaggcgcaat
     9361 tccggcacgt accgaccgcg ttcgtgttca ttacaccggt aaactgatcg acggcaccgt
     9421 gtttgacagc tccgttgctc gtggtgaacc cgctgaattt ccggttaatg gcgtgatccc
     9481 tggctggatt gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc
     9541 gcaggaactg gcatatggcg agcgcggcgc aggcgcatcc atccctccgt tcagcaccct
     9601 ggtgtttgaa gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgaacc
     9661 agaggggagc aggcattcag caataaaccc ttcagtttgc caaacggcgc tattttgtgt
     9721 tgcaaagacc ccgtaagcgt gtatttttgt gagctgtttc gcgttatcac cgtgatatga
     9781 cactcacttt aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt
     9841 gttacctaat atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc
     9901 gtagagcctg aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa
     9961 gtcgttgccg atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga
    10021 catattcagc ttattgccat tggcggtgcc attggtacgg ggttgtttat ggggtctggc
    10081 aaaacgatta gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg
    10141 ctctttttcg tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct
    10201 tttagtgact tcgcttccga tttactcggg ccgtgggcag gatatttcac tggctggact
    10261 tactggttct gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct
    10321 cagttctggt tccccgatct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg
    10381 ctgacgctca accttgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg
    10441 atcaaaatcg tcgccatcgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac
    10501 tttcagtcac cgaccggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg
    10561 ttcccgaaag gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg
    10621 gggattgagc tggtaggtac aacagctgcg gaaaccaaag atccggagaa atcactgcca
    10681 cgcgcgatta actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg
    10741 attatgtccg tgacgccgtg gagttcggta gtcccggaga agagcccgtt tgttgaactg
    10801 tttgtgttgg taggtttgcc tgcggctgcc agcgtgatca actttgtggt gctgacctct
    10861 gcggcgtctt ccgctaacag cggcgtcttc tctaccagcc gtatgctgtt tggtctggcg
    10921 caggaaggtg tggcaccgaa agcgttcgct aaactttcta agcgcgcagt acccgcgaaa
    10981 gggttgacct tctcgtgtat ctgtctgctc ggcggcgtgg tgatgctgta tgtgaatccc
    11041 agcgtgattg gcgcgttcac gatgatcacg accgtttccg cgattctgtt tatgttcgtc
    11101 tggacgatta tcctttgctc gtacctggtg taccgcaaac agcgtcctca tctgcatgag
    11161 aagtcgatct ataagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcgttcttt
    11221 gtgttcgtgg tcgtgttgct gacactggaa gatgacactc gccaggcgct gttggtcacc
    11281 ccgctgtggt ttatcgcact ggggctgggc tggttgttta ttggtaagaa gcgggctgct
    11341 gaactgcgga aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat
    11401 tactcacccg ccagcgcgcg tgggaacagt acattgtttt ccaggctgat gtgatccatc
    11461 aggtcatcaa tcagttcatt aatgccgtta tacatcgctt tccaggtggt gcaggcttct
    11521 ggcggcggtg tgacgttatt ggtagtgtgt ttgatcactt ccagcagttc gcccgcttca
    11581 tcgtgctcgc tttccattac gctgattggc cccattgcct ggctgcccat gccttgtttg
    11641 atcatcggga agaggatctg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc
    11701 atagtcaggt attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgctcg
    11761 actttagtcg cttgcaggat cagctccggc agctgctcgc ggtgacgatc gtggtagcgc
    11821 acgatgatat ggtcaatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt
    11881 tgttcagcaa gctttgccag ttcagcttca atgacctcaa catccagttc tttacgtgcc
    11941 gccgcgcgcg ccagcgtctg cttaccgcca cagcagtaat ccatatcata tttacgaaac
    12001 agagccgaag cgcgagggat agagagcgcc agttcaccta aaggttggtc gcgataagcc
    12061 atagctgata cctcattctt aataatataa gatgtatttt aaatgcatct ttaaggcaaa
    12121 agctataacc cttacgtagc agaggcttat ttaactcact gccagcgtgt caactttctg
    12181 agacagattc agttaaaggt ttttctggtt ttgctctgac cgcaatgacc acgccaatca
    12241 ctaacagcgc gataccgctc agcgtcatta gcggcggcat ttgctggcgg agtaaaaagg
    12301 tgtacagcaa acctgccagc gtttcgaaaa caatcagcgg cccgagaatc actgtcggta
    12361 atcgctggct ggcgacgttc cagcagagtg cgccaaccca tgagcaaagc acggctatcg
    12421 caaccatcag actaataaac accagcggac gggggccaaa aggtaaggag aagtccggcg
    12481 tttgtatatt cagccagtaa caggcgacga gatagccgat gagagaaacc ggcagcgtga
    12541 ccagcgcctg cgccgtcgcc cacatcatcg gatatttgtc gggattttcc cgcagccagc
    12601 gggcgttgcg cagggcatac catgcccagc agaccacgga aactaacgct agcacgatgc
    12661 cagaggtata acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca
    12721 cactcgccag gccgatgcca atacaaatca gtgccggggc gagttttccc cacgcgagtt
    12781 tgccgtcgcg ctggctataa agcagattgg caaagacagg aatgaccacc ggcagggtgc
    12841 cgataatcat cgtggaaaca ggcgcgccag tacgttgaat ggcactggca aggcagaagt
    12901 aataaatgag gttgcccatc atagtgagca tcaaggcggt aagccagtcc cgacgcgcca
    12961 actgacgcag acgcacgcgt cccagccagg caatgggcag cgcaattaac cctaacgcca
    13021 gataacgccc catcgactgc aacatcgccg ggtattccgg tacgatcaac ggcccgacaa
    13081 aaataagccc ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg
    13141 gcatccattg gtctgtcaga agaaagttca ggaagcagac atgattgctc ccggcatccc
    13201 gtcattatta gagaggatgc gggaggatgc cactttaatt aactagttat taacattaaa
    13261 aagatggctt acgctttcgg ctaacgttgt cgttgggcgg ccaatcaatt tgctaagcgt
    13321 tttgctgtta tcaaacagac cacctttcga tgcgccaacg tcagaatccg ccagcatatc
    13381 cgccagtccg tcgggcagtc cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag
    13441 attttgatag gtaacctgtt tgccgctctg tttggtcagc tctgccgcta actgtgtcaa
    13501 tgtccaggca ctatcgcccg ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat
    13561 cacgcgtgcc gcagctgccg cataatctgc ccgcgttgct gaggcgattt tgccatcgcc
    13621 cgccgcaccg ataaatacgc cgtgttccag tgctgccggg gcgctggcga ggtagttttc
    13681 gctgtaccag ccgttgcgca gcagggtgta aacgatgcca gaatcagcca acattttctc
    13741 cgtctcgatg tgctcatcgg cgaggccgag cggggaggta tctgcatgta gcaggctggt
    13801 ataagcgata aatttcacac cagccgcctt tgcggcatta ataacattac gatgctgcgg
    13861 ggcacgttga cccacttcgc tggaagagat cagcagtagt ttttccactc cctgaagtgc
    13921 agatgtcagt gcggcttcat cgccgtagtc agcctgacgc acggtaatgc cttgtgctgc
    13981 cagagcctgg gcttttgccg gattacgaac gatagccact atttggctga caggaaccgt
    14041 tttcttcaag gattcaataa catagtgacc aagttggcca gtggcaccag taatagcgat
    14101 catgggaagt ctccatcgtt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt
    14161 tagtaagtac gcacaaaaag gtaagtatga aatgagtcag gttagcctgt cgcaacaact
    14221 gaaagagggt aatctctttg cggaacagtg cccgtcgcgc gaggtgttga aacacgtcac
    14281 cagccgttgg ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga
    14341 cctgcggcgc aaaatcggcg gggtgagcga aaagatgctt gcgcagtcgt tacaggcgtt
    14401 agaacaggat ggttttctta accgtatcgc gtatccggtg gtgccgccgc atgtggaata
    14461 tagcctcacg ccgctgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat
    14521 tgagttgaat ttgcccgagg tgttggcggt gcgggatgaa cgtgcggcat aactgccgga
    14581 tgcgctgagc ttatctggtc tacggtgcat ggcttgtagg gcggataaga tgcgccagca
    14641 tcgcatccgc caataaatgc cggatgcggt tgatgattta taggccgaaa aagaagtgtt
    14701 ttacttactc aaatccacct gataaatcgc aaacccgata tcatcggtcg cgactttatt
    14761 catcggatat tgccctttct ctttaataaa cgctgcggct ttatctgacg gagaagtttc
    14821 gaaacggata tccagtttct tatcgccagc tatcggtgct aaacgccagt tgttatctgc
    14881 cgccgggtga atttcccccg cacgtttcga ctcatcagca atccacgctg ccagcaccga
    14941 gcggttctca tccggtgaag caaaagcgat atggctgtcg cccgtaccgg caaatttgcc
    15001 gccgtaagcg cgatagttat tggtggcaac gaggaacatg gcgttcggat caatcggctt
    15061 gccattaaag gtcaggttct taatcctttc cgcattggca ttaatcatct ggcactcgcc
    15121 gtcataacgg gcaggctggg taacatcaat ctgataattc acaccatcaa tcacatcaaa
    15181 gttataggtg cggaaaccat cccagttgat gagtgattgc ggtttcgtgc tgttaggatc
    15241 aatctggtta aactgtccgg cggagcactc cagccactct ttcacctctt taccgctggc
    15301 tttcactaca atcagcgtat tggggtagag ataaagatcg gcggcattac ggaaggtcaa
    15361 ctggcctttt tccacctcca catagcttgc cgggtcattt ttgcgaccac caactttaaa
    15421 cggtgcggca gctgaaagca ccggcagttt tgccagatcc ggatcgccct gaatgtaatg
    15481 ttcgacatac gctttttgcg cgttgttcac tacttgcacg gtcggatcgt cctgcaccag
    15541 cgccagatag ctatacatat tgtcggcaga tttaccgatt ggcttgctga cgaactggcg
    15601 tgtggcatcg tgatcggctt tgagtgtttc taccagcttg ctgtcttccg ccgtgaggga
    15661 ttttttattg gcgatgtcgt aaatcggccg agcttccgct ttcgcctgcg tcacctgcca
    15721 tttaccgctg tcattactga gttgtaagtc gaccacccca agatgatcgc cccacatgcc
    15781 cggcattacc gccggaacac cattcagcgt gcctttggtg atatcagccc cttcgatatc
    15841 agcaaaatct ttacccggga aaacggcgtg agcatgacca aacataatgg cgttaacgcc
    15901 cggaatttca ctgaggtaat aaactgagtt ttccgccatc actttatacg gatcggcaga
    15961 cagcccggaa tgtgccagaa cgacaacgac atcggcacct ttctcgcgca tttcaggcac
    16021 gtatttgcgc acggtttcgg taatatcatt caccgtcact ttcccggata aattagcttt
    16081 atcccagccc atgatttgcg gcggtacgac gccaatatag ccaatcttca gcgtctgttt
    16141 ttttccgtct ttatcgacca cttcggtgtc tttaattaaa tacggtgtaa acattggctg
    16201 tttggttctg gcgtcaatga cgttggcatt tacataaggg aatttcgctc ccgccagcgc
    16261 atttttcagg taatccagac cgtagttaaa ctcatggttg ccaagcgtgc cgacggtata
    16321 gtccagcgta tttaatgcct tatacactgg gtgaatatca cctgctttta atcctttcgc
    16381 cgacatgtaa tcggccagcg gactcccctg aatcaaatcg ccgttatcga ccagtacgct
    16441 gtttttcact tcattgcggg catcgttaat caggcttgcc gtacgtacca gtccgaattt
    16501 ttccgtggcg gtgtctttgt aataatcgaa atccatcatg ttgctatgca gatcagtggt
    16561 ttccatgata cgcagatcga ccgtcgctgc attcacactg gcggtaatca gcgtggccag
    16621 gagcgttgcg ctaaacttaa tcatcaggga catcctttta tcatcgggaa tacgaaagaa
    16681 aagggagaat aaacgtctta cttatagaac agtgaagaat gccacaattt tacgctttga
    16741 aaatgatgac actatcacag ttggcgcatt cattaacgat agggtataag taaaacaata
    16801 agttaacacc gctcacagag acgaggtgga gaaatgttag atcaagtatg ccagcttgca
    16861 cggaatgcag gcgatgccat tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc
    16921 agcaaagcgg acaattctcc ggtaacggca gcggatattg ccgctcacac cgttatcatg
    16981 gacggtttac gtacgctgac accggatatt ccggtccttt ctgaagaaga tcctcccggt
    17041 tgggaagtcc gtcagcactg gcagcgttac tggctggtag acccgctgga tggtactaaa
    17101 gagtttatta aacgcaatgg cgaattcacc gttaacattg cgctcattga ccatggcaaa
    17161 ccgattttag gtgtggtgta tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc
    17221 aaggcctgga aagaagagtg cggtgtgcgc aagcagattc aggtccgcga tgcgcgcccg
    17281 ccgctggtgg ttatcagccg ttcccatgca gatgcggagc tgaaagagta tctgcaacag
    17341 cttggcgaac atcagaccac gtccatcggc tcttcgctga aattctgcct ggtggcggaa
    17401 ggacaggcgc agctgtaccc gcgcttcgga ccaacgaata tttgggacac cgccgctgga
    17461 catgctgtgg ccgcagctgc cggagcgcac gttcacgact ggcagggtaa accgctggat
    17521 tacactccgc gcgagtcgtt cctgaatcct gggttcagag tgtctattta ttaattaatt
    17581 tatgcagcag gtccatcact tgctgcacct cttcctgagt aagcgccccg tctttggccc
    17641 attgcacgcg accgtcttta ttcagcacca ctacagcgga acttttttca tccagctgcc
    17701 aggcaccgcg tgcgacgcca ttgctatcaa caataaactg cgaccaggga taaagctttt
    17761 tattactctc cagactgctg cgcacaaaca tgccggaacc cggaattgcg tcgtcggtgt
    17821 taacaatggt ggtggtctgg taacgatcgt gcggtaactt cgctgattta atcgcttcaa
    17881 tcagcgtcgc gtttttctct tttgcagagg tgcgaccagc aatatgttgc agtactcgca
    17941 cttttcccac taactgcgcg ctgttccagg ttttgtagct aaactgatct ttatcaagca
    18001 ccaactcgcc acgatcggtg atgccaatcg gcggcactcg ctgaccggtt tcgaactgat
    18061 gtgcggaagc catcatcggc aacagcaggc aggtgagtgc cagaatcttg cgtagggtca
    18121 tggtgtttcc ttcttatgat atgcaggtga tccgaccact tgggtctgag ttttaatcat
    18181 atgtgctatt tatcgaattt cccgcaagtg tgatgccagt ttgcggtcaa gcgcacaaat
    18241 catatgaaaa atgaatgctt atactgaaga ccgcgcttcg gtaaaaagat aattctgaat
    18301 aattgtaacc tttaggtaaa aaaagttata cgcggtggaa acattgcccg gatagtctat
    18361 agtcactagg cattaaaatt tgcgcctcat aatagttggg ccgattgtgg caccgcacag
    18421 gcgtaatact cagcaggaga taacaatgaa aattttccaa cggtacaacc cacttcaggt
    18481 ggcgaagtac gtaaagatcc tgttccgtgg acggttatac atcaaggacg ttggcgcttt
    18541 tgaatttgat aagggtaaga ttcttatccc aaaagtgaag gataagctgc atttgtcagt
    18601 gatgtccgaa gttaaccgtc aggttatgcg tctgcaaaca gagatggctt aaccaaagtg
    18661 ctatgcagta ataagacggc tcctgattca ggagccgttg atgtttctgg gggttacgcg
    18721 acgctttctt ctttatcttt agcgtcaggc agttttggcg aaagggcggt ggccttgctg
    18781 tcgatccggg tcaccagcag ctggtcgatg cggtagttat cgatatccac cacttcaaat
    18841 ttgtagccgg cgaatttcac cgaatcggtg cgtttcggga tcttacgcag cataaacatc
    18901 ataaagcctc cgatggtttc gtagttgccc gactgcggga actcgtcaat atccagcacg
    18961 cgcatgacgt cgtcaattgg ggtgccgccg tcaatcagcc atgagttctc atcacgggcg
    19021 acaatctgct cttccagccc ctgaccgacc agatcgccca tcagcgtggt catcacgtcg
    19081 ttgagggtga tgatccccac caccagcgcg tactcgttca tgatcaccgc gaagtcttca
    19141 cctgcggttt taaaactttc caacgcctct gaaagggtta acgtatccgg cacaatcagc
    19201 gtgttgcgaa tttgtacgcc gctgttcagt gccaggcttt ggttagccag cacgcggttc
    19261 agcaggtctt tagaatcgac ataaccgatg atgtggtcaa tatcttcatt acagacgagg
    19321 aacttagagt gcggatgttc cgccacctta ttcttcaggc tttgctcatc ttcgtggaga
    19381 tcaaaccaaa tcacgttttc acgcggtgtc attgaagacg gaacggtacg ggattccagc
    19441 tcaaagacgt tttcaatcag ctcgtgttcc tgtttacgta acacgcccgc cagcgcaccg
    19501 gcttccacta ccgcgtagat gtcatcagaa gtgatgtcat ctttacgtac cattggcagt
    19561 ttgaaaatac ggaagatcat gttcgccagg ccgttgaaga accacaccag cggggtgcaa
    19621 acgtacaggc agaagcgcat cgggttgatg atacgcaaag ccaccgcttc tggcgcaatc
    19681 ataccgatgc gtttcggggt taaatccgca aacaggataa acatgccagt cactaacgag
    19741 aaagagagaa taaagctcag ttgctcagag agttctgccg acatatagcg ggagaacagg
    19801 ctgtgaaaag ctggagaaaa tgccgcatca ccgacgatac cgccgagaat cgcaactgcg
    19861 ttcagaccga tttggaccac cgtaaagaac atgccgggat tttcctgcat attcagaacg
    19921 cgttgggcat ttatattgcc ttcatcagcc agcagtttaa gtttgatttt gcgtgaggcg
    19981 gcaagtgaga tctcagacat cgagaagaac gcacttacag cgatcaagca gagtatgact
    20041 aaaatactgt ttaacatatc ttatccgacc tttcaggcca gatcctcgga aggggaagtt
    20101 gattaatttg tgtgaaatac acattgaaag ccgattcgaa gaaagtgaac cggcattttc
    20161 agtggctagt atagcgtaag gtactgtaaa gccgccagag ggttaaaatt cagacagctg
    20221 aaaaatgcaa aactgcctga tgcgctatgc cgatcaggcc tacgcagtcc ttgcaatata
    20281 ttgaatttgg acgattttgt aggcctggca aggcgtttac gccgcatccg gcataaacaa
    20341 aacgcacttt atcaacaatc tacatttata cccgtaacgc tatgcttccg gcggcagaca
    20401 gacgccaatt ccgccaattc cacagtagcc atacgggttt ttatgcagat attgctggtg
    20461 gtcatcctcg gcataataaa acggtgtggc gttagcgatt tccgtggtga tgcgacgatc
    20521 gtcatcggcg gcaagcatcg ccgcctgaaa acgttccaga ctggcgcgag ctgcggcatc
    20581 ctgttctggg gtcagcggat aaatcgctga acgatactgc gtgccttggt cattgccctg
    20641 acgcattccc tgggcaggat cgtgattctc ccaaaatacc tgtagcaact gctcatagct
    20701 gatgacggaa ggatcgtaaa caatgcgtac cgcttcggca tgaccagtat caccggagca
    20761 cacttcccga taagtcggat ttggcgtata gccgccggta taacctgcgg cggtgctgta
    20821 aacgccgggt aactgccaga acagacgctc cacaccccag aaacaaccca tcgcaaaaat
    20881 ggcaatctcc attccgtcag gtacattggt cattgagtga ccgttgaccg catgcagcgt
    20941 ggctacgggc atcggggtgt tacgtccagg cagggcatcg gcgggggaaa caagatgctt
    21001 tttatcaaat aaactcatgg tgtcgctctc ccgaaaatcg gtcattgggg ttaaggttgt
    21061 aacaagaggc gtatttgcac acaataacca ccgtgaatag gtctaaagta aaacataaga
    21121 aatatttggg gtttagtctg ctttttaatc catattactg gatttttgtt aagccgttta
    21181 acggcgttcc aggggcagga aaaaaggata ttcaggagaa aatgtgcgct atatccgaca
    21241 gttatgctgt gtaagcttac tctgcttaag cggatctgcc gtcgccgcga acgtccgtct
    21301 acaggtcgag gggttatcgg gacagctgga aaagaacgtt cgtgcgcagc tttctacgat
    21361 tgaaagtgat gaagtgacgc cagaccgtcg ctttcgcgca cgcgtcgatg atgccatccg
    21421 cgaaggtctg aaagcgctgg gttattacca gccgaccatt gaatttgatc tccgtccacc
    21481 gccaaagaaa gggcggcagg tattgatcgc caaagttacg ccaggcgtgc cggtgttaat
    21541 tggcggcacc gatgtggtat tgcgcggcgg cgcgcggacc gataaagact atttgaaatt
    21601 gctcgatact cgcccggcta ttggcacggt actgaaccag ggcgattatg aaaatttcaa
    21661 aaagtcctta accagcattg cgttgcgtaa aggttatttc gatagcgaat ttaccaaagc
    21721 gcagctgggc attgcgctcg gcctgcataa agccttctgg gatattgatt ataacagtgg
    21781 cgaacgttac cgctttgggc atgtgacctt tgaaggatca caaatccgcg atgaatacct
    21841 gcaaaatctg gtgccgttta aagagggcga tgagtacgaa tcgaaagatc tggcggaact
    21901 gaaccgccga ctttctgcta ccggctggtt taactcggtg gtggtggctc cacaatttga
    21961 taaagcgcgc gaaacgaaag tattaccctt gacgggcgtg gtttcgccgc gaactgaaaa
    22021 caccatcgaa accggggtcg gttactctac ggacgtggga ccgcgcgtga aagcgacgtg
    22081 gaaaaagccg tggatgaact cttatggtca cagtctgacc accagtacca gtatttccgc
    22141 gccggaacag accctcgact tcagctataa aatgccgctg ctgaagaatc cactggaaca
    22201 atattatttg gtgcagggcg gttttaagcg cactgacctg aacgataccg aatctgactc
    22261 cactacgctg gtggcttctc gctactggga tctctccagc ggctggcagc gtgccattaa
    22321 cctgcgctgg agcctcgacc actttaccca gggtgaaatt accaacacca cgatgctgtt
    22381 ttatcctggg gtgatgatta gccgcacgcg ttctcgtggt ggcctgatgc caacctgggg
    22441 cgactcgcaa cgctactcta tcgactactc caacacggcc tggggctcag atgtcgattt
    22501 ctccgttttc caggcgcaga acgtctggat ccgcacactg tacgatcgcc atcgttttgt
    22561 gacacgcggc acgctgggct ggattgaaac gggtgatttc gacaaagtac cgccggatct
    22621 gcgtttcttc gccgggggcg atcgcagtat tcgcggctac aaatacaaat ctatcgcccc
    22681 gaaatacgcc aacggtgacc tgaaaggggc ctcgaagctg ataaccggat cgctggaata
    22741 ccagtacaac gtgaccggaa aatggtgggg cgcggtgttt gtcgatagtg gcgaagcggt
    22801 aagcgatatt cgccgcagcg actttaaaac cggtaccggg gtcggcgtac gctgggaatc
    22861 gccggtcggg ccaatcaaac tcgattttgc cgtaccggtc gcggataaag acgagcacgg
    22921 gttacagttt tacatcggtc tggggccaga attatgagtt tatggaaaaa aatcagcctc
    22981 ggcgtggtta tcgttatctt actgttgctg ggatcggtgg cgtttctggt gggcaccacc
    23041 agcggcctgc atcttgtatt taaagcggcg gatcgctggg tgccaggact ggatattggc
    23101 aaggtcaccg gcggctggcg cgatctcacc ttgtctgacg ttcgttatga gcagccaggc
    23161 gtggcggtaa aagcgggtaa tctgcatctg gcggtcggtc ttgagtgcct gtggaacagt
    23221 agcgtttgta ttaatgacct ggcgctgaaa gacattcagg tcaacatcga cagtaaaaaa
    23281 atgccccctt ctgaacaggt tgaagaagag gaagatagcg gtccgctgga tctctccacg
    23341 ccgtatccca tcaccctgac acgggtggcg ctggataacg tcaacatcaa gattgatgac
    23401 accacggtat cggtgatgga cttcacctcc ggcctgaact ggcaggagaa aaccctgacc
    23461 ctgaaaccga cgtcgctgaa aggcctgttg attgccctgc cgaaagtggc ggaagtggcg
    23521 caggaagaag tggtcgaacc gaaaattgaa aatccgcagc cggatgataa gccgctcggc
    23581 gaaacgttga aagatctctt ttctcgtccg gtattgccgg aaatgaccga cgtgcatttg
    23641 ccgcttaacc tgaacattga agagtttaag ggcgagcagc tgcgcgtgac gggcgacacg
    23701 gacatcaccg tgaacaccat gctgctgaaa gtaagcagca tcgacggtaa tactaaactg
    23761 gacgccctgg atatcgattc caatcaaggg atcgtcaacg ccagcggcac ggcacagctg
    23821 tcagacaact ggccggtgga tatcaccctc aacagcacac taaacgtgga gccgttgaaa
    23881 ggtgaaaaag tgaagctgaa agtgggcggc gcgctgcgcg aacagctgga gattggcgtt
    23941 aacctttccg gtccggtgga tatggattta cgcgcccaga cgcgactggc ggaagctgga
    24001 ttgccgctca acgtggaagt gaacagcaaa cagctttact ggccgttcac tggtgagaag
    24061 cagtatcagg cggatgatct gaaactgaaa cttaccggca aaatgaccga ttacacgctc
    24121 tctatgcgta cggcagtgaa gggacaggag atcccgcccg cgactattac cctcgacgcg
    24181 aaaggtaatg aacagcaggt caatctcgac aaactcaccg ttgcggcgct ggaaggcaaa
    24241 actgaactca aggcgttgct cgactggcaa caggccatta gttggcgcgg tgagctaacg
    24301 cttaatggca ttaacaccgc caaagagttc ccggagtggc cgtcgaaact caatggcttg
    24361 attaaaaccc gcggtagcct gtacggcggc acctggcaga tggaggtgcc agaactgaag
    24421 ctgaccggta acgtcaaaca gaacaaagtg aacgttgacg gcacgctgaa aggcaacagt
    24481 tatatgcagt ggatgatccc agggcttcat ctggaactgg ggccaaacag tgccgaagtg
    24541 aaaggcgagc tgggggtaaa agatctcaat cttgatgcca ccatcaacgc gccggggctg
    24601 gataacgcgc tgccggggct tggcggtaca gcgaaagggc tggtgaaagt acgcggcacg
    24661 gtggaagcgc cacaactact ggcagatatc accgcgcgcg gcctgcgctg gcaggaactt
    24721 tccgtggcgc aggttcgcgt ggaaggcgac attaaatcca ccgatcagat cgccgggaaa
    24781 ctcgacgtac gcgttgagca aatttcgcag ccggatgtaa atatcaacct cgtcaccctg
    24841 aatgccaaag gcagcgaaaa gcagcacgag ctacagttgc ggattcaggg cgagcctgtc
    24901 tccgggcagc ttaatctggc aggaagtttt gatcgcaaag aagaacgctg gaagggaact
    24961 cttagcaata cccgcttcca gacgccggtc ggcccgtggt cgctgacccg cgatattgcg
    25021 ctggattacc gcaataagga gcaaaaaatc agcatcgggc cacactgttg gcttaacccg
    25081 aatgcggagc tgtgcgtgcc gcaaactatc gatgcggggg ccgaagggcg tgcggtggtg
    25141 aatctcaacc gcttcgacct cgccatgctg aaaccgttta tgccagaaac cactcaggcc
    25201 agcggtatct tcacgggtaa agcggatgtt gcctgggaca ccacgaaaga ggggctgccg
    25261 cagggcagta tcaccctttc ggggcgtaac gtgcaggtaa cgcaaaccgt caacgatgcg
    25321 gcgctgcccg tggcgtttca gacgctgaat ctgacggcgg aattgcgtaa caaccgtgcc
    25381 gaattgggct ggaccatccg cctgaccaat aacggccagt ttgatggaca ggtgcaggtg
    25441 accgatccgc aaggccgccg taatcttggt ggcaacgtca atatccgtaa cttcaacctt
    25501 gcgatgataa accccatctt tacccgtggg gaaaaagcag cggggatggt gagtgccaac
    25561 ttgcgtctgg gtggtgatgt gcaaagcccg cagttgtttg gtcagcttca ggttacgggt
    25621 gtggatatcg acggcaactt tatgccgttt gatatgcaac cgagccagct tgcggtcaac
    25681 tttaacggta tgcgctcgac gcttgccggt acagtacgga cccagcaggg tgaaatctac
    25741 ctgaacggtg atgccgactg gagccaaatt gaaaactggc gggcgcgggt aacggcgaaa
    25801 ggcagtaaag tgcggatcac cgtgccgccg atggtacgaa tggatgtatc gccagatgtt
    25861 gtattcgagg ctacaccaaa cctgtttacc ctcgatggtc gcgtggatgt cccttgggcg
    25921 cgcatcgtgg tgcacgatct gccggaaagc gcagtaggcg tctccagcga tgtggtgatg
    25981 cttaacgata acctgcaacc ggaagagccg aaaacggcgt cgattccgat taacagtaac
    26041 ctgattgtcc acgttggcaa taatgtgcgc attgacgcct ttggcctgaa agcgcggctg
    26101 acgggcgatc ttaacgtcgt tcaggacaaa caagggctgg gcctgaacgg gcagatcaac
    26161 atccctgaag ggcgcttcca tgcctatggt caggatctga ttgtgcgtaa gggtgagtta
    26221 ctgttctctg gtccgccgga tcaaccgtat cttaatattg aagctattcg taacccggat
    26281 gctacagaag acgacgtaat cgccggagtt cgcgtcactg gtctggcgga cgaaccgaaa
    26341 gcggagatct tctctgaccc ggcgatgtcg caacaagctg cattgtctta tttgctacgt
    26401 ggacaagggc tggagaacga tcagagcgac agtgcggcaa tgacctcgat gctgattggt
    26461 ctgggggttg cgcaaagtgg ccagattgtg ggtaaaatcg gcgagacgtt tggcgtaagc
    26521 aatttagcgc tcgacaccca gggagtaggc gactcctccc aggtagtggt cagcggctat
    26581 gtattgccag gtctgcaagt gaaatatggc gtgggtatat ttgactctat agcaacactc
    26641 acgttacgtt atcgcctgat gcctaagcta tatctggaag ccgtgtctgg tgtagaccag
    26701 gcactggatt tgctctatca gttcgagttt tagcaatgcg aatatttgtc tacggcagtt
    26761 tacgccacaa acaaggcaac agtcactgga tgaccaatgc ccagttactg ggcgatttca
    26821 gtatcgataa ctaccagttg tatagcctgg gccactatcc aggcgcagtt ccggggaacg
    26881 gaacagtaca cggtgaagtt tatcgtattg acaacgccac gctggccgaa cttgatgcct
    26941 tgcgcaccag gggcggtgaa tacgcgcgcc agttgattca gacgccgtac gggagtgcat
    27001 ggatgtacgt ttatcaacga cccgtcgatg gattaaagct aattgaaagc ggcgactggt
    27061 tagacaggga taagtaacca tatgcatacg ccaccttcgg gtggcgttgt tttttgcgag
    27121 acgactcgca ttctgttttg taattccctc accctttgct tttctctccg agccgctttc
    27181 catatctgtt aacgcataaa aaactctgct ggcgttcaca aatgcgcagg ggtaaaacgt
    27241 ttcctgtagc accgtgagtt atactttgta taacttaagg aggtgcagat gcgtattacc
    27301 ataaaaagat gggggaacag tgcaggtatg gtcattccca atatcgtaat gaaagaactt
    27361 aacttacagc cggggcagag cgtggaagcg caggtgagca acaaccaact gattctgaca
    27421 cccatctcca ggcgctactc gcttgatgaa ctgctggcac agtgtgacat gaacgccgcg
    27481 gaacttagcg agcaggatgt ctggggtaaa tccacccctg cgggtgacga aatatggtaa
    27541 agaaaagtga atttgaacgg ggagacattg tgctggttgg ctttgatcca gcaagcggcc
    27601 atgaacagca aggtgctggt cgacctgcgc ttgtgctctc cgttcaagcc tttaatcaac
    27661 tgggaatgac gctggtggcc cccattacgc agggcggaaa ttttgcccgt tatgccggat
    27721 ttagcgttcc tttacattgc gaagaaggcg atgtgcacgg cgtggtgctg gtgaatcagg
    27781 tgcggatgat ggatctacgc gcccggctgg caaagcgtat tggtctggct gcggatgagg
    27841 tggtggaaga ggcgttatta cgcttgcagg cggtggtgga ataaggtgtg tttatttatc
    27901 gctggcataa aaaaaccctt actaaccgaa gctcggcgtt cagggttatt gcgctagaag
    27961 aacttattta ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc
    28021 gttttcccaa ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa
    28081 gaagtgagcg atctgcgctt tcagcagctc tggcagatcg ttaacgtctt taatgtgatc
    28141 gtattctttg ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc
    28201 gtcggtcatt ttcagaacgc caaccggacg gcaacggatc acagaacccg gctgcagcgg
    28261 gtacggagtc gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat
    28321 gtaaccgtag ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc
    28381 gccgctctct ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac
    28441 aacgtagatg tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt
    28501 ttcctttaaa aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt
    28561 ctttgcttgt tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca
    28621 ggaaaatcca taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt
    28681 gattaacgtt ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat
    28741 tgtctatagt tttttcgcga acatcttttc accaataata attaccccga cgaggacaac
    28801 cctatgtgga aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg
    28861 ttggccgctc cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgct
    28921 gcagaaacca atgtagcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgct
    28981 gatggtcagc aaaagcagga aaaccagatt aaagcggtac gttccttcgt tgcacaaggg
    29041 gtggatgcga tctttatcgc tccggtggtc gcgacaggtt gggaaccggt attaaaagag
    29101 gcgaaagatg ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa
    29161 tctctctata tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac
    29221 tggctggtaa aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt
    29281 ggggccagcg tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca
    29341 aatatcaaaa tcatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtt
    29401 atggagagct ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat
    29461 aacgatgaca tggtgattgg cgcaattcag gcaattaaag aagcgggcct gaaaccgggc
    29521 aaagatatcc tcacgggttc cattgacggt gtacccgata tctacaaagc gatgattgat
    29581 ggcgaagcga acgccagtgt tgaactgacg ccgaatatgg caggtcccgc cttcgacgcg
    29641 ctggagaaat acaaaaaaga cggcaccatg cctgaaaagc tgacgctgac caaatccacc
    29701 ctttatctgc ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga
    29761 gggttgctat gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg
    29821 gtggtgcgct gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg
    29881 ctttacaccg ggggaaacca tgacgactaa ccaacaccag gagatcctcc gcaccgaagg
    29941 attaagtaaa tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg
    30001 tggcgaaatc atggcgctgc tcggtgaaaa cggggcggga aaatcgacgc taatcaaagc
    30061 attaaccggt gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggctatctc
    30121 accgaaaaat accgcccatg cacaacaact cggcatcgga accgtttatc aggaagtcaa
    30181 cctgctaccc aatatgtcgg tcgctgataa tctattcata ggtcgcgaac ccaaacgttt
    30241 cggctttcta cgccgcaaag agatggaaaa gcgtgccacc gaactgatga catcttacgg
    30301 tttctccctc gacgtgcgtg aaccgcttaa ccgtttttcg gtggcgatgc agcaaatcgt
    30361 cgccatttgc cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc
    30421 cagcctcgac acccaggaag tagagttact gtttggcctg atgcgtcagt tgcgcgatcg
    30481 cggcgtcagc ctgatctttg tcactcactt tctcgatcag gtctatcagg tcagcgatcg
    30541 gatcaccgtc ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca
    30601 gatcgaactg gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc
    30661 cgggcgaaca ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg
    30721 aacgatcgca ccgtttgatc tcgaagtacg ccccggcgag atcgtcggcc tggcaggctt
    30781 gctgggatcc ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag
    30841 cggcacggcg ttgatcaaag gcaaacagca aaacctgcga tcgccacatc aggcttcggt
    30901 actgggcatc ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ccgccgcctc
    30961 ggtacgggaa aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc
    31021 ccgcaaagag cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc
    31081 ttcaactgaa caaccgattg aatttctctc tggcggcaat cagcaaaaag tgttgctttc
    31141 acgttggcta ctgacccgac cgcaatttct gatcctcgac gagccaaccc gcggcattga
    31201 tgttggtgcc cacgccgaga tcatccgcct gattgaaacg ctgtgtgctg acggtctggc
    31261 gctgttggtg atctcctccg aactggaaga gctggtgggc tatgccgatc gggtgattat
    31321 catgcgtgat cgcaaacagg tggcggagat cccgctggca gagctttccg ttccggcgat
    31381 catgaacgcc attgcggcgt aaggagaaca gtgtgatgcc tcaatctctc ccggacacta
    31441 cgccgccgaa aaggcgcttt cgctggccaa cgggaatgcc gcagctggcg gcgctattgc
    31501 tggtgctgct ggtcgatagc ttggtagctc cgcatttctg gcaggtggtg cttcaggatg
    31561 ggcgtttgtt cggtagcccc atagacattc ttaaccgtgc agcacccgtt gcgttactgg
    31621 cgattggtat gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga
    31681 tggctatcgc cggagccaca acggctgcga tgacggtcgc gggagtcagc ctgccgattg
    31741 ttttgttaag cgccctgggc acaggcatcc tggcgggatt gtggaacggc atactggtag
    31801 cgatcctcaa aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg
    31861 tggcgcaact gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt
    31921 ttggcagtgg atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc
    31981 tcctgttctg gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta
    32041 tcaacattcg ggcggcaaaa aatgccgggg taaacacgcg aatcatcgtc atgctcacct
    32101 acgtgttgag cgggctatgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg
    32161 gtgccgatgc caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga
    32221 ttggcggcgg atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc
    32281 tgattattca ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc
    32341 aggtggtgaa agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca
    32401 gtctgattaa aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc
    32461 ggcgtctttg tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca
    32521 agagtgatct gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg
    32581 acctttgtga tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcttttacc
    32641 ggcgtgtttc tggcaaaagt gattggcgac ttcggcctct cgccgttgct ggcatttccg
    32701 ctggtgctgg taatgggctg tgcctttggc gcatttatgg ggcttctgat cgacgccctg
    32761 aagatcccgg catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat
    32821 ctcgtttcgg aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt
    32881 gcgtggaaaa tccctggcgg cggtcgctta agtgcgatgg ggctgctgat gctggcggtg
    32941 gtggttatcg gcatattcct cgcgcatcgt acccgttttg gtaatcaggt atacgccatt
    33001 ggcggcaacg caacgtcggc aaacctgatg gggatttcta cccgcagcac cactattcgc
    33061 atttatatgc tctccaccgg actggcaacg ctggcgggga ttgtcttctc gatttatacc
    33121 caggccggat atgcgctggc gggcgtaggt gtggaactgg acgctatcgc ctcagtggtg
    33181 attggcggta cgcttttgag cggtggcgtt ggaacggtat tagggacgct ttttggcgtg
    33241 gcgattcagg gactgattca gacttacata aactttgatg gcacgctgag ttcctggtgg
    33301 acgaaaatcg ccatcggtat tttgttgttt atttttatag cattacagcg tggattaacg
    33361 gtgctgtggg agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcaataa
    33421 aaacgcctct ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag
    33481 cgttcgacat cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcagg
    33541 gtttccggga taatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt
    33601 tccgccagga atgccatcgg gttgcactca tacagtaaac gcagtttacc gtccgggtgg
    33661 ctggcggtgc ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca
    33721 accagtgaac cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg
    33781 cagaatttaa tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg
    33841 gagtaggttt tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc
    33901 agcgaaggat cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatagta
    33961 gaggagccgt ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct
    34021 tcttccgtta ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg
    34081 acagagacgt taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat
    34141 tttgcgtgtt cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatgccc
    34201 gcaacgatat cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt
    34261 ttctgctgaa cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt
    34321 cctgctttgt tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa
    34381 gcagtgagct caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct
    34441 aacgttttca taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta
    34501 acaaataatc tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga
    34561 aatgcgtttc tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag
    34621 tgacgtatgc gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg
    34681 ctggcgcgcc agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg
    34741 agcaccttac ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc
    34801 gatccgcagc cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg
    34861 gaagcggtac tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt
    34921 gtgctgcgcg accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg
    34981 ggaatggcga cctggattct ggaacagtgt ggttacaaac cgggctttgt aatcggcggt
    35041 gtgccgggga actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa
    35101 gcggatgagt atgactgcgc cttcttcgac aaacgctcta aatttgttca ttactgcccg
    35161 cgtacgctga tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa
    35221 gcgatccaga aacagttcca ccatctggtg cgtatcgtac cggggcatgg ccgtattatc
    35281 tggccggaaa atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag
    35341 gagctggtgg gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa
    35401 tgggaagtct tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa
    35461 cataatatgc acaatggcct gatggcgatt gcggcggctc gccatgttgg tgtagcgccg
    35521 gcagatgccg ctaatgcgct gggttcgttt attaacgctc gtcgccgtct ggaactgcgt
    35581 ggtgaagcga atggcgtcac ggtatatgat gatttcgccc atcacccaac ggcgattctg
    35641 gcaacgctgg cggcgctgcg tggcaaagtt ggcggtacgg cgcgcattat tgctgtgctg
    35701 gaaccgcgct cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta
    35761 ggccgtgccg atgaagtctt cctgctgcaa ccgacgcata ttccgtggca ggtggcagaa
    35821 gtggcagaag cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat
    35881 atggtggtga aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt
    35941 ggtggcatcc atcagaaact gctggatggt ctggcgaaga aagcggaagc cgcgcagtaa
    36001 ttcggcctta gcctgatata gtgtgcgtag gccggataag gcgttcacgc cgcatccggc
    36061 atttgagcat agtgcctgat gcgacgcttg atgcgtctta tcaggcctac aggtgcaccg
    36121 catccggcaa accatactca catcaacaac gaaaattacc cttcattctc cgccagttcg
    36181 cgcagatact ggaaaatctg gcgtgcggat ttcggcggct tattcccttc tttctctttc
    36241 ttcgcgttgc ggatcagagt acgcagctgc tgacgatcgg catcgggcca cagatttaat
    36301 acctcagcga tggcgtcatc accttgatcg atcagacgat cgcgcaggtt ttccagttta
    36361 tggaacagca ccacctgctg gttgtgacgg tttttcagct tatccagcgc ctgacggatc
    36421 ggttctacat cgcgctggcg cagcatctta ccgatgagct gcaactggcg gcggcgacct
    36481 tccatcttaa tacgctgggc gagctcaata gccgcgcgca gatccgcatc taacgggatc
    36541 ttatccagcg cgtttttccc cagatcaaca atttccgcgc caaggcgttt tagctcctcg
    36601 gcatcacgtt taatttcact tttactgacc cagataattt catcgtcttc gtcttcgatg
    36661 tcatcaccgg gaacgtcgtc gagccagtct tcgggctgct tagtcatctc aggctcctta
    36721 aaaaaagagg ctaatgttac cagttaagat gcgcactgaa aaacggttct ctgttagact
    36781 tcagagaaac tctctacatt atggcacttg caatgaaagt aatctctcaa gttgaagcgc
    36841 agcgcaagat tctggaagaa gcagtttcca ctgcgctgga gttggcctca ggcaaatcgg
    36901 acggtgcgga agttgccgtc agcaagacca ccggcattag cgtaagcacg cgttatggtg
    36961 aagtggagaa tgttgaattc aatagcgatg gcgcgctggg gatcactgtt tatcaccaga
    37021 accgcaaagg tagcgcatca tccaccgatt taagcccgca ggccattgcc cgtactgtac
    37081 aggcggcgct ggatattgcc cgttatacct cgccagatcc ctgtgccggc gtggcagaca
    37141 aagagctgct ggcctttgac gcaccagatc tcgacttgtt ccaccctgcg gaagtttccc
    37201 cggatgaagc cattgaactg gcggcccgcg cagaacaggc ggcattgcag gcggacaaac
    37261 gcattaccaa taccgaaggt ggcagcttta acagccacta cggtgtcaaa gtttttggca
    37321 acagccacgg catgttgcag ggttactgct caacgcgtca ttcgctctcc agctgtgtaa
    37381 ttgccgaaga aaatggcgat atggagcgtg attacgccta caccattggc cgcgcgatga
    37441 gcgatctgca aacgccagag tgggtcgggg ccgactgtgc tcgccgtact ttatcgcgcc
    37501 tgtcaccgcg taaactctcc accatgaaag cgccagtcat ttttgccaat gaagtggcaa
    37561 ccgggctttt tggccatctg gtgggggcga tagcgggtgg ctcggtttac cgtaaatcta
    37621 cctttctgct ggattcgctg ggtaaacaaa ttctgccgga ctggctgacc attgaagagc
    37681 atccgcatct gctgaaaggg ctggcgtcga cgccattcga cagcgaaggt gtgcgcaccg
    37741 agcgtcgcga tattattaaa gatggcatcc tgactcagtg gctgctgacc agctactcgg
    37801 cgcggaaact ggggctgaaa agcaccggac atgcgggcgg tattcacaac tggcggattg
    37861 ccggacaagg gctaagcttc gaacaaatgc tcaaagagat gggcaccggg ctggtggtga
    37921 cggagctgat gggccagggc gtgagtgcca ttaccggtga ttattcccgt ggtgcagcgg
    37981 gcttctgggt agagaatggc gaaattcagt atcccgttag tgaaatcacc atcgcaggta
    38041 atttaaaaga tatgtggcgc aatattgtca ccgtcggtaa cgatattgaa acacgcagta
    38101 atatacagtg tggttctgtg ctgttgccgg agatgaaaat cgccgggcag taaaaaactg
    38161 gcgcgacctg ccgcgctata aactaaaaaa ttacacaaaa tcattcgcac tgcatcgaga
    38221 cggaaagtga atgagggccg gggagcgtac tcgcagtacg tgacccgggc gaatgagcgt
    38281 agccgtcgat gaggcagtgt gaaggatgaa gtgtaaataa aaaaggaagt gagcaatgcg
    38341 taaaagcctg ttagctattc ttgcagtctc ctcgttggta ttcagttctg cgtcgtttgc
    38401 cgctgatctt gaagacaata tggaaaccct caacgacaat ttaaaagtga tcgaaaaagc
    38461 ggataacgcg gcgcaagtca aagacgcgtt aacgaagatg cgcgccgcag cgctggatgc
    38521 gcaaaaagca acgccgccga agctcgaaga taaatcaccg gacagcccgg aaatgaaaga
    38581 tttccgccac ggtttcgaca ttctggtcgg tcagattgac gacgcgctga agctggcaaa
    38641 tgaaggtaaa gtaaaagaag cgcaggctgc tgcagagcaa ctgaaaacga cccgcaacgc
    38701 ctatcaccag aagtatcgtt aattcctcat ttccctgttg cctgcactca ggtaacaggg
    38761 aacttatcat cgcaaatgat gcaccacctg attgctgctg ccgcgccaga tcagggaagg
    38821 gtcttttaaa tcctgcacaa atttgccgtc gaccagcacg ttaatcaaat caacaacctg
    38881 catttgcgca gcgttgagtt cgtcgagttt atagcctgtc cacacccaga tgtctttacc
    38941 cggacactcg gcgcggatgc gttttaccag tttcagaata tccggcacgt tttgcggatg
    39001 cagcggatcg ccgccggaga gggaaatacc ctggcgtttg atacgagtgt cattcagatc
    39061 gttaataatc tggtcttcca ttgctttagt aaatgtctga ccggaattta cccgccatgt
    39121 gcttttgtta tagcaaccgg ggcattcatg aacacacccg gagacaaaca gggtgcagcg
    39181 agtgccgggg ccgttgacga tgtcgacagg atagtactga tgataattca ttgtttcgcc
    39241 tggcttatca gtccgcgtat aggatgcatc cggtaatcaa tgcctgatgc gacgctgtcg
    39301 cgtcttatca ggcctacagc cgttgccaaa tgtaggccgg ataaggcgtt tacgccgcat
    39361 ccggcatttg cttagcgcag agtgaagatt aacctatctg cccattcccc aaatgtttaa
    39421 cgcggcgctt aacttcttcc tgcttaccag cgttaaatgg acgtgcatcc gggctaccta
    39481 aatatccgca cacgcggcga gtcaccgaca cacgggaggc gtcatggtta ccacatttcg
    39541 ggcaagtgaa gcctttgctg gtgcactcga actcaccggt aaagccacac tcgtagcact
    39601 catcaatcgg tgtattggtg ccgtaatacg gtacatgctg atagctgtaa tcccagacat
    39661 cttccagcgc cttcaggttg tgctgaatgt ttggatactc gccgtagcaa atgaaaccac
    39721 cgttcgccag cggcgggtaa ggcgcttcaa agtcgatctt gtcgtacggg ttcaccttct
    39781 tctccacatc gaggtggaaa ctgttggtgt agtaaccttt atcggtcacg cccggcacca
    39841 cgccaaactc agcagtatcg agacggcaga agcggtcgca caggttttca ctcggcgtgc
    39901 tgtagagact gaaaccataa cccgtttctt ctttccactg atccactgcc tggcgcagac
    39961 gttcaacaat ggcgatacct ttcgcgcgaa gctgctcgtt gtcgtaaaca tgctcgccgc
    40021 cgaacagcgc gttaatggtt tcgtggatgc cgatgtaacc cagcgaaata gacgcacgac
    40081 cgtttttgaa gatttcagaa acatcatcgt cagcattcag acgtacgcca caagcacctt
    40141 ccatatagag gatcggggcg acgcgcgctt tcacgccttc gagacgagcg atacgggtca
    40201 tcagtgcctt acgtgccagc accagacgtt catccagcag cttccagaag gtggcttcat
    40261 cgccttttgc ttccagagca atacgcggca ggttcaggct gatcacgccg aggttgttac
    40321 gaccatcgtg gatctgctcg ccgttttcat tttcccacac gccgaggaag ctgcggcagc
    40381 ccatcggggt tttaaacgaa ccggtgactt tcactacctg atcgtagttc aggatatccg
    40441 gatacatgcg cttgcttgcg cactccagcg ccagctgttt gatgtcgtag ttcggatcgc
    40501 cttttttatg gttcaggcca tcgcgaatcg caaacaccag tttcgggaac accgcagttt
    40561 tacggttttt accgaggcct gcgatacggt tacgcaggat cgattcctga atcaggcgcg
    40621 attcccagct ggtgcccaga ccaaaaccaa aggttacaaa cggcgtctga ccgttggcgg
    40681 tgtgcagggt gtttacttcg tactccagcg actggaaggc atcgtagcac tctttgatgg
    40741 tacgagagtt agcgtagcct tcggcgtccg ggatgttcca ctcttctgcg gtcttgcgat
    40801 gtttgttgta gctggcggta acaaacggcg ccagcacttc atcgatacgg ttaatggtgg
    40861 tgccgccata aatatggctg gcgacctgag caataatctg cgcagttacc gcggttgccg
    40921 tagagatcga cttcggcggt tcaatctcgg cgttccccat tttaaagccc tgggtcagca
    40981 tgcctttcag gtcgatcaac atgcagttga acatcgggaa gaacggcgag taatcgagat
    41041 cgtgatagtg aatatcgcca cgctcatgtg cctgcaccac gtcacgcggc agcaggtgct
    41101 ggcgtgcata gtgtttagcc acgatcccgg ccagcaggtc gcgctgggtt ggaatcacct
    41161 tgctgtcttt gttggcgttt tcgttgagta acgaggcgtt ggtctgctcg accagaccac
    41221 ggatctcctg gttcaggcga ccgcgttttt cacgttcaat gtcgcgatcg tgacggtact
    41281 cgatgtaagc acgagccagt tgtttgtatg gacccgacat cagctggttt tcaactgcgg
    41341 tctggatctc gttgatatcc acctggttgc ggccctgcat ctgctcgctg acaaccgcgg
    41401 caacagtggc gcaataatcg gcatcatcga cttccgctgc tttagctgca cgcagaatcg
    41461 cttctttgat gcgctctgat ttaaacggca ctttgcagcc gtctcgtttc atcacatgcg
    41521 gtgtcatgat cgctccatat ttttaagaac aggttatcca cagaaattgg gaaaggcgtt
    41581 ttccggtttt ttcgtttcgt tttccggtgc tttccgcaat cgccatccgc ttatccacaa
    41641 cgccgcacct ttttcgtggg gagctgtagt agcaattata gtcgattaat acaacatatt
    41701 gggttgggac gcattttaaa gtctatatat agtgctttgc atcaaggatg tttgaacttt
    41761 ttttgatgta gctcaaagta aaaagcagag cgtacggatg acgggcgcta cagcgttatg
    41821 taaatttttt aatgaatttg ctggttgaaa aatcaacaaa aacaacatgc tgacagacaa
    41881 aaccccggaa tgaccggcat taccggggct tagggaagat ttacttctgt aaccaccaga
    41941 cagcctcaaa aggccgtaaa ttcatggcac agggttgtgg tgaggcttct tcgtagttat
    42001 gcatcacaag ctgccagttg ccgcgcattt gccctggctg ccagggttgg atctcacggc
    42061 taaggttggc aatgaccagc aaggtttgcc ccttccattc acggcgatag caccacaata
    42121 cagggctgtt tggcagcaga tcctggtaat tgccccatgt caggatggct tcttgcttac
    42181 gcagtgcgat taacttttgg taggtgtaaa acaccgagga ctcatcggcc agcgcggctt
    42241 ctacgttgat ttgttgatag ttatcgccca ggccaatcca cggttcgcca gccgtaaacc
    42301 cggcattatc gccgttgctc cattgcatgg gcgtgcggct gttgtcacgg gatttactgg
    42361 cgaggattgc caataactcg tcggcatcac gaccatcgtt gcgcagctcg gcaaacatat
    42421 tgaggctctc cacgtcgcga tagtcagtaa tgcgcgtgaa atgcgggttg gtcatgccaa
    42481 tctcttcgcc ctggtagata tacggcgttc cctgcatgcc atgcagcacc atcgccagca
    42541 tttttgccgc aggcacgcgg tattcacctt catcaccaaa gcgagaaaca atgcgcggct
    42601 gatcgtggtt acaccagaac aaggcattcc atgccacgtt gtgcattcct tgttgccagt
    42661 ggcggaacaa tgttttcaac gccacaaagt caggtttagc cagcgtccat ttttcaccac
    42721 cgggataatc gaccttcagg tgatgaaaat taaaggtcat cgacaattca ctgcctgtca
    42781 gtgccgcgta tcgctggcaa tgctcaaggc tggtggagga catttcacct acggtcatta
    42841 acccgcgtgg cgtaaacaca tcgcggttca tctcgtgcaa aaactcgtgt gctcgtggcc
    42901 cgtcggtgta gaagcgacgc ccgtcgccat ccaggtcttc agggaaacgc gggtctttgg
    42961 agatcagatt caccacatcc aggcgcaacc cgtcgacccc acgatcggcc cagaattcac
    43021 agactttttt cagctctgcg cgtaccgctg gattctccca gttgagatcc gcctgttctg
    43081 gtgcaaagag atgcaaatag tactgttcgc tttccgcatg ccagcgccac gcactaccgc
    43141 caaattttga acgccagttg ttcggtggcg tttctggttc gccatcgcgc cagatataaa
    43201 actggcggta agggctttct ttgttcagcg cctcgcgaaa ccaggcatgt tgggtagagg
    43261 tatggttaaa caccatatcg agaatgatac gaatcccgcg cgattttgcc tgcgtcacca
    43321 gttcgtcaaa atcgtccagc gtgccgtaga tgggatcaat cgccgtatag ttcgctacgt
    43381 cgtaaccgtt atcgacctgg ggagagatat aaaagggggt tagccagatg gcatcaacgc
    43441 ccagtttatg cagatagtcc aggcgttgga taacgccacg taaatcgccg gtgccgctac
    43501 ccgtggtgtc ctgaaaactc tttggataaa tctggtagat aacgccgttt tgccaccagt
    43561 ggggaagatt agtcattacg ttattcctgc aattgcgccc cgaagaaaat taaacaatat
    43621 ccagcgtgcc caggcggtat ttccgctgat agataaacga ggtgagtaca atcgggatga
    43681 tgatggcgat aaccattgcc agcgcaaata cctgccagta gctcggttga atcgagagaa
    43741 ttcccggcag gccgcctacg ccgataccat tcgccataac gccgttcagg ccgcatagta
    43801 atcctgccag accagaacca atcatcgcgc acagcatcgg gaagcgatat ttcaggttga
    43861 tgccgtacat tgcaggctca gtgaccccaa gccaggcgga gatagcggca ggcacggaga
    43921 tctcgcgttc attgtgcttg cggctggaaa tgataatgcc tatcacggcg gagccctgag
    43981 cgatattcga cagcgcaatc agcggccaca ctggcgtgcc acccatgctt tgaatcatct
    44041 gcaaatcaat agcaagggtg gtctggtgta caccggtgat caccagcggg gcgtacagga
    44101 agccaaacaa tgctgcgcca atcggagcaa agctgccggt catcaggtga cgtaccgcaa
    44161 aggcaacgcc atcgccaatc atgcgaccaa acggaccaat cagcgcatgg gcgaggaaca
    44221 ccgcgaggat cagcgaacag acgggcacca ccaccagata gaggtaatcc ggcacgatgc
    44281 gtttaaggcg agtttcaata acgcccagcg ccagcccggc taacagtgcc gggatcacct
    44341 gcgcctgata gcctactttg gcgatgctga acatgccaaa gtcccacact tccggcagct
    44401 gctgcccgag cagataagcg ttcatcagct ggggagaaac cagtgtcacg ccaagcacga
    44461 taccaaggat cggcgtgccg cccatttttt tcaccgctga ccagcaaatc ccgaccggca
    44521 ggtagaagaa gatcgcttca ccgatcaacc acagaaaatc gtagatcgtt tgcagggaag
    44581 ggtacatttg cgccagcgtt tgaccgttgc tcatgggcaa atcgccgatc acattgcgaa
    44641 aaccgaggat caaaccgccg ctaatcaacg cgggcagcaa cgggaagaag atctccgcga
    44701 aatgagagat caactgctca tgccatttca tattctgccg ggcggctttt tttacctgct
    44761 ctttatcaac ctgcgcctgt ccggttgacg ctattagtgc ttgatagtaa tcacccacgt
    44821 tggtgccaat caccacctga aattgcccgg cattggtgaa acagcctttc accatgggga
    44881 gttgctcaat ttctttcggt ctggcattgg ccggttggtt gaggacaaag cgcaggcgag
    44941 taatacagtg gctcaccgtc gcaatattgc cgcgcccgcc gaccagttca atcaaccgat
    45001 cgatatccgt ttggtttatt ttgctcatca taaagcccca tggcagatga catttttggt
    45061 tggctgcaga atatattgcg cggaaaaatt taaaaacggg aacgttcccg aagcgcagcg
    45121 aagatcacaa tttatcgttc aggaaacgat caggacaggg tggcggggat gatgatttgt
    45181 tgcggttcgc tgcgcccggt tacctgtgcg atcaattggc aggccgcctg gcgtccagct
    45241 tcggcgtaac caggatctac ggtgacgatc tccggatgaa ggaatttcat taatggcgta
    45301 ttaccgacgc tcgccagttg caaggtgtcg atgcgttgct cttgcaggta tttacttgcg
    45361 ccaagtgcca gcgtgtcggt tgcgcacagt aaggcggtag tttcaggcgt aatcactttt
    45421 gctacgtttt catagccttg cttcatagca agccctggca gagcggcaac gggatgcagt
    45481 ttatgcgctt tgcagaacgc caggtaggct tcgtgacgtc gcttcccggt cgtcacgtca
    45541 ctatgcggca cgccgagata actgatattg cgatgcccct ggtcatagag ccgttgcatc
    45601 aggatcttga ttgccccttc gtcgtcataa cagaccgaag caaagccttt tgcgtcacgc
    45661 gccagcagaa ccagcgatga ctgccagtgg gctaacattt cttctgttat gccagtaaaa
    45721 ccgaacaaca ctacgccgtc gatattacgc cgtttcagca cccccaaatg ttcggcaact
    45781 aattgcgggg aaaactgact ttccatcatg attgggtcgt aaccttgttc atagaacgct
    45841 ggcagcatgg tttgaacagc gagattttct gacaacgaat ccaggcgggt aacaatgatg
    45901 gcgaccactt tatcgctttg cccacgcata gcgcgcgcag agcgggaagg ggaaaatcca
    45961 tgctgattca tcactgcttc aacacgctcg cgggtgcgct ggctcacgcc gctttcgtta
    46021 ttcagcaccc gggaaaccgt agatttcccc acgccgctta agcgcgcgat gtctttgatg
    46081 gtcagccgat tttgcatcct gttgtcctgt aacgtgttgt ttaattattt gagcctaact
    46141 ttacccgtgc attcagcaat gggtaaagtc tggtttatcg ttggtttagt tgccagcagg
    46201 tattatatcg ccatagatgc tacgaatatt attggattct ccttattatt tgcggcgctt
    46261 ttttcactta ccggaggtta tatggaacct gatcccacgc ctctccctcg acggagatta
    46321 aaacttttcc ggtaagcccg tcttttcacg gcgttaccgg atgcgtaagg ccgtgacgtt
    46381 ttaacgtccc tgctcagctt tattaccttc aggtaaggct tcgccacgcc tgaagacatt
    46441 tctgtactgt ttcagacagt gcggagggac tccttatgtt taaagaaatt tttacccggc
    46501 tcattcgcca tttaccttcc cgtctggttc atcgcgatcc attgcctggc gcgcagcaga
    46561 cagtgaatac ggcggtcccg ccgtccttaa gtgcgcattg cctgaaaatg gcggtgatgc
    46621 ccgaagaaga attatggaaa acgttcgaca cccatccgga agggttaaat caggcggaag
    46681 tggaatctgc ccgcgaacaa catggtgaaa ataaattacc cgcacaacaa ccgtcgccgt
    46741 ggtgggtaca tttatgggtc tgctatcgca acccctttaa tattttactc accattctcg
    46801 gcgctatttc ttacgccacg gaagatttat ttgccgcggg cgttattgcg ctaatggtcg
    46861 ctatttcaac gttactgaac tttattcagg aagcacgttc cactaaagcg gcagatgccc
    46921 tgaaagcgat ggtcagcaat accgcgacgg tgctgcgcgt aattaacgac aaaggcgaaa
    46981 atggctggct ggagatcccg atcgaccagc tggtgcccgg cgatattata aaactggcgg
    47041 cgggagatat gatcccggca gatttacgta tcttgcaggc gcgggatctg ttcgtcgctc
    47101 aggcgtcgtt aaccggtgag tctctgcccg tggaaaaagc cgctaccact cgccagccgg
    47161 agcacagcaa tccgctggag tgcgacacgc tgtgttttat gggcaccacc gtggtgagcg
    47221 gcacggcaca agcaatggtg attgctacag gtgccaatac ctggtttggt caactggcgg
    47281 ggcgtgttag tgagcaggaa agcgagccga atgcctttca gcaggggatc agccgcgtca
    47341 gtatgctgct gattcgcttt atgctggtga tggctccggt ggtgttgctt atcaacggtt
    47401 acaccaaagg cgactggtgg gaagcggcac tgtttgcgct ttcggtcgcg gtaggcttaa
    47461 cgccggaaat gttgccgatg attgttacct cgacgctggc gcgcggcgca gtaaagctgt
    47521 cgaaacagaa agtgatcgtc aaacatctgg atgctattca gaactttggc gcaatggata
    47581 ttctgtgcac tgataaaact ggcaccctga cacaggacaa aattgtgctg gagaatcata
    47641 ccgatatctc cggtaaaacc agcgaacgcg tgctgcatag cgcgtggttg aacagtcatt
    47701 atcagaccgg acttaaaaac ctgctcgata cagcggtgct cgaaggtacg gatgaagagt
    47761 cagcgcgctc gctggccagt cgttggcaaa aaattgatga gattccgttt gatttcgagc
    47821 gtcgccggat gtcggtggta gtggcagaaa ataccgagca ccatcagctg gtttgcaaag
    47881 gtgcattgca ggaaatcctc aatgtgtgtt cgcaggtgcg tcacaatggc gagattgtgc
    47941 cgctcgatga catcatgctg cgtaagatta agcgggttac tgatacgctt aatcgtcagg
    48001 ggctgcgcgt ggttgcggtg gcgacgaaat acctgccagc gcgtgaagga gattaccagc
    48061 gggcggatga atccgacctg atcctcgaag gatatattgc tttccttgat ccgccaaaag
    48121 agacaaccgc tccggcactg aaggcattaa aagcgagtgg aatcaccgta aaaatcctta
    48181 ctggcgacag tgagttagtt gcagcgaaag tgtgccatga agtggggctg gacgcgggag
    48241 aggtggtcat tggtagtgat attgaaacgc tatctgacga cgaactggca aatctcgcac
    48301 agcgcaccac gctgtttgcc cgcctgacgc cgatgcataa agaacgcatt gtgaccttac
    48361 tgaagcgcga agggcatgtg gttggcttta tgggcgatgg tattaatgat gcgcccgcct
    48421 tacgcgctgc ggatatcggc atttctgtgg acggcgcggt agatattgcc cgtgaagcgg
    48481 ctgatatcat cctgctggaa aaaagcctga tggtgctgga agagggggtt attgaaggac
    48541 gtcgcacttt cgccaacatg ctgaaataca tcaaaatgac ggcgagctcc aacttcggta
    48601 atgtgttcag cgtgctggtg gcgagtgctt tcttgccctt cctgccgatg ttgccgttac
    48661 acttgcttat tcagaacctg ctgtatgatg tgtcacaggt ggcgatcccg tttgataacg
    48721 tcgacgacga gcaaattcaa aagccgcagc gttggaatcc ggcggatctg gggcgcttta
    48781 tgatcttctt cggaccgatc agctcgatct tcgatattct gacgttttgc ctgatgtggt
    48841 gggtattcca tgccaacacg ccggaaacgc aaacgctgtt ccagtcggga tggtttgtgg
    48901 tgggcttact gtcgcaaacg ctgattgtgc atatgatccg cacccgccgt gtgccgttta
    48961 ttcagagctg tgcatcgtgg ccgttaatga tcatgaccgt gatcgtgatg attgtcggga
    49021 tcgcattgcc gttttcaccg ctggccagtt atctgcagct gcaggcgctg ccgttaagct
    49081 atttcccgtg gctggttgcg attctggcag ggtatatgac attaactcag ttggtgaaag
    49141 gattttatag ccgtcgttac ggctggcagt aaagaataaa ctgggcacga tagccccgat
    49201 tcagattgca gacaaagtaa gctttgttca tgccggatgc ggcgtaaatg ccttatccgg
    49261 ccaacaaatt caataaattg caggaatcac gtaggcctga taagcgtagc gcatcaggcg
    49321 attttgcgtt tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta
    49381 gcgacgaaca gcgatcgctt cgatctcaat cttcacgtct ttcggcagac gggcaacttc
    49441 aacgcaagaa cgtgccggga aggtggcatt gtgttcggtg aagaaggctt cgtaggtagc
    49501 attaacggtt gcgaagtcgt tcagatcttt cacaaacacg gtggttttaa cgatgtcgcc
    49561 cactttcagg cctgcggctt cgacgatcgc ttttacgtta tccagcgact gacgtgcctg
    49621 tgcagcgacg tctgccggta cttcgcccgt tttcggattt accgggatct ggccggaggt
    49681 gatgatcata ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt
    49741 ttccgtcgcg atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct
    49801 cattataggg agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg
    49861 ggaaaactct ttttcacagt atttgcattt gagcgcgatg tcattggcgc gttttcgcac
    49921 ggcaaagctg gaagaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag
    49981 cacattgtcg atgcgctctg gcagactggg gcgcgattta cccaccactt catagttgtc
    50041 gatacggtta accgtggctt gcggcgcata caatgccagt tgatctacct gatcttcact
    50101 caaaaaggta ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaagtt
    50161 cagaccgatg gtgatgcgct gatccgtttc ggtcagcttg aacagactca acagcttaaa
    50221 accgatctgg gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa
    50281 tttattatcg tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag
    50341 tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc tgctggaagt accaggcgtg
    50401 tggcgtttta tcaacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt
    50461 catattggct ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt
    50521 ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac
    50581 ttccgctatc acttcttcaa tagagctgtg cagcctccat gcgatccctt tttcatcgag
    50641 catatccaga atgtattgcg gcattgccag cgcgtccggc gcgatgaagt aaaaacggtt
    50701 gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg gtgcggccat atttcaggtc
    50761 accaaccatt gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tagtgaataa
    50821 gtccagcaag gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac
    50881 attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat
    50941 cgcatcgacg taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cctttttacc
    51001 cagtgatgta ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcataga
    51061 tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt
    51121 gtgcttcaac agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag
    51181 attaaggtca tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt
    51241 agccatcttt tatctcctga cgcctgggca aaaaaaagcc cctcgattga ggggctggga
    51301 atgggtgatc aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac
    51361 aaaatgtcga acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat
    51421 cgtcagaagc ggtgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa
    51481 ttataagtca aaatacatgc atttttattt gttgtgattc tgcaggggaa cggtcgttcg
    51541 gtatcaataa ttgcagacat ttcctgcttt tttctttttc actttcacgc aatcagatat
    51601 gcattttatt ccattcatcc gacttatagg gcggagtgtg aaaacgaacg gctaacacta
    51661 ttgcttactg ctcagggatg cgcgctatca ctttaatttc aaaatcaaag cctgccagcc
    51721 atgtaacacc taccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca
    51781 ccgtcatgat gtcttcaaat tggttttctg gatcggtatg gaagctcgta acatcaatga
    51841 tatcgtcaaa agtgcatccc gcagcagcca gggtcgcatg caaattatca aatgccagtc
    51901 tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg
    51961 aaacaaacag caaatcgccg gagcgaatag ccgcagaata acgatgttca gcatacagtg
    52021 aatgtcggcc agcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta
    52081 cgacatgaac agaagattta tatttaccag gagccgcttt tagcggacga cgtgagtaaa
    52141 caaaacccag acatcatggg aaatggctgg gcttaattga gcgtagtcgg ttatgcgcca
    52201 aacgcgccat caatggtatg catcgcgccg gtaacaaagc tggcttctgg ccctgctaac
    52261 catgcgacca taccagcgac ctcttccggt tgcccatgtc ttttgatagc cataaaacca
    52321 tgcaacatat cgcgcattgg cccgttggcg ggattagcgt cggtatcaat tggccctggc
    52381 tggacgacgt tgatggtgat cccacgcggt ccaaaatcac gggccagccc gcgcgccatg
    52441 ccttgcaggg cagatttgct ggcggcataa gcagccatgc ctgcaacagg catacgatcg
    52501 ccattcacgg agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca
    52561 acggaggcat ggtaaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca
    52621 tttaattcca gggcatcgcc aaagacgcca atacctgcat ttaccacgag gatatccaat
    52681 gcgccgctct tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact
    52741 gctgtcgctc ccgtctcttg tgccaggcgt tcagcggcat ctttcgaccc cgcataggtg
    52801 aatcgtacat tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacgg
    52861 ctaccaccga ggatgagaac tgtcttacct gtaaaagcgc ccataaggac tccttgattt
    52921 attatgtaac atgcattaca aaactgtttt aactttctgt caacaagttt tgtaatggtc
    52981 actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc
    53041 tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg ctgtcagtgt tgctgaagtt
    53101 actgattatc ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg
    53161 ttatttagcc gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt
    53221 cttcttgatg atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc
    53281 agatatagcc aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat
    53341 gatccacaag cgcgtgatat tgccgttcaa tattatcacg ccgcagaaac gaccatttat
    53401 gactatatcg ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc
    53461 gtgatgtcag ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca
    53521 acagctgcac tggcggggga agcgataaaa actattctca aggagtgatg cgggccttga
    53581 cccgaaaggc gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa
    53641 ggctagcgga attgatcatc ttttcgtata acgatagaaa tgaaacgttg ttttaattaa
    53701 ggagtggaaa agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag
    53761 ttacgccagg cgattgagca tatcaaagca cacgttacgg tagaaacgcc aaagggcaag
    53821 cacgatatcg aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac
    53881 gaagctcgcc gtgcggagta ccatgcccgc tatctcgaca ttcagattgt gttaaaaggt
    53941 caggaaggca tgaccttcag cacacaacct gcaggtacgc cggataccga ctggttagct
    54001 gataaagaca tcgcattttt gccggaaggc gttgatgaga aaacagttat ccttaatgaa
    54061 ggtgattttg ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggtgca
    54121 ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta
    54181 aataactcca ggtttacggc gagtttgtga aaagagcgtt ttttgatatt ttttgtgagt
    54241 aaaatttgta atgcttagac gttcttatta actcaaggag tccgtcatga gcaaaatatc
    54301 aggttggaat ttttctcaaa acattacatc agccgacaat tgtaaacaaa aaaatgaaga
    54361 cttagatacc tggtatgtgg gaatgaatga ttttgcccga attgccggag ggcagaatag
    54421 cagaagcaat attctttctc ccagagcatt tttggagttt ttggctaaga tatttaccct
    54481 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcataa
    54541 taagtcctca tcttatatca aaaataatga tggtagtgag ataatgaagt ttgttatgaa
    54601 taatcctgaa ggggagcgag cggatttatc taaggtggaa atagaaatta cacttgcctc
    54661 agcttttaat aatgggattc gtgaaggaca tacagtcatt atatttacac aacctgatgg
    54721 ttcgactaat cgttatgaag ggaagtcctt tgaaagaaaa gatgagagtt cattacacct
    54781 gattactaac aagattctgg cgtgttacca acgcgaagct aacaaggaaa tagctcgtct
    54841 attaaatatt ccgcaggagt taaataattc tcaggacttg aataactcgc aggtgagttg
    54901 taaatattca gttgattcta cgattacgga tttattagaa aaaccattga ataatgcatt
    54961 attagcaata aggaaagaac atctgctatt aatgccttat gtatgtaatg aatcgatttc
    55021 atacttgctt ggtgaaaaag ggatactgaa agaaatagat gaccttaacg cagtaaataa
    55081 ttatcttctt aataataaga aagctactga caatgaaatt aatgacatca aagttaacct
    55141 gtctcatatt cttattgat
//
LOCUS       DAZFVO020000009        54584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFVO020000009 DAZFVO020000000
VERSION     DAZFVO020000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 54584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.009"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            300..386
                     /gene="leuL"
                     /locus_tag="ACHKNB_RS02410"
                     /old_locus_tag="pgaptmp_000482"
     CDS             300..386
                     /gene="leuL"
                     /locus_tag="ACHKNB_RS02410"
                     /old_locus_tag="pgaptmp_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HGU4194061.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            479..2050
                     /gene="leuA"
                     /locus_tag="ACHKNB_RS02415"
                     /old_locus_tag="pgaptmp_000483"
     CDS             479..2050
                     /gene="leuA"
                     /locus_tag="ACHKNB_RS02415"
                     /old_locus_tag="pgaptmp_000483"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HGU4194062.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2050..3141
                     /gene="leuB"
                     /locus_tag="ACHKNB_RS02420"
                     /old_locus_tag="pgaptmp_000484"
     CDS             2050..3141
                     /gene="leuB"
                     /locus_tag="ACHKNB_RS02420"
                     /old_locus_tag="pgaptmp_000484"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGU4194063.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3144..4544
                     /gene="leuC"
                     /locus_tag="ACHKNB_RS02425"
                     /old_locus_tag="pgaptmp_000485"
     CDS             3144..4544
                     /gene="leuC"
                     /locus_tag="ACHKNB_RS02425"
                     /old_locus_tag="pgaptmp_000485"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HGU4194064.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4555..5160
                     /gene="leuD"
                     /locus_tag="ACHKNB_RS02430"
                     /old_locus_tag="pgaptmp_000486"
     CDS             4555..5160
                     /gene="leuD"
                     /locus_tag="ACHKNB_RS02430"
                     /old_locus_tag="pgaptmp_000486"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGU4194065.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5209..6387)
                     /gene="setA"
                     /locus_tag="ACHKNB_RS02435"
                     /old_locus_tag="pgaptmp_000487"
     CDS             complement(5209..6387)
                     /gene="setA"
                     /locus_tag="ACHKNB_RS02435"
                     /old_locus_tag="pgaptmp_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HGU4194066.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6489..6620)
                     /gene="sgrT"
                     /locus_tag="ACHKNB_RS02440"
                     /old_locus_tag="pgaptmp_000488"
     CDS             complement(6489..6620)
                     /gene="sgrT"
                     /locus_tag="ACHKNB_RS02440"
                     /old_locus_tag="pgaptmp_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HGU4194067.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6709..8364
                     /gene="sgrR"
                     /locus_tag="ACHKNB_RS02445"
                     /old_locus_tag="pgaptmp_000489"
     CDS             6709..8364
                     /gene="sgrR"
                     /locus_tag="ACHKNB_RS02445"
                     /old_locus_tag="pgaptmp_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HGU4194068.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8398..8497
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8528..9511
                     /gene="thiB"
                     /locus_tag="ACHKNB_RS02450"
                     /old_locus_tag="pgaptmp_000490"
     CDS             8528..9511
                     /gene="thiB"
                     /locus_tag="ACHKNB_RS02450"
                     /old_locus_tag="pgaptmp_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HGU4194069.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9487..11097
                     /gene="thiP"
                     /locus_tag="ACHKNB_RS02455"
                     /old_locus_tag="pgaptmp_000491"
     CDS             9487..11097
                     /gene="thiP"
                     /locus_tag="ACHKNB_RS02455"
                     /old_locus_tag="pgaptmp_000491"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HGU4194070.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11081..11779
                     /gene="thiQ"
                     /locus_tag="ACHKNB_RS02460"
                     /old_locus_tag="pgaptmp_000492"
     CDS             11081..11779
                     /gene="thiQ"
                     /locus_tag="ACHKNB_RS02460"
                     /old_locus_tag="pgaptmp_000492"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HGU4194071.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11893..12657)
                     /gene="yabI"
                     /locus_tag="ACHKNB_RS02465"
                     /old_locus_tag="pgaptmp_000493"
     CDS             complement(11893..12657)
                     /gene="yabI"
                     /locus_tag="ACHKNB_RS02465"
                     /old_locus_tag="pgaptmp_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4194072.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12743..13621)
                     /gene="araC"
                     /locus_tag="ACHKNB_RS02470"
                     /old_locus_tag="pgaptmp_000494"
     CDS             complement(12743..13621)
                     /gene="araC"
                     /locus_tag="ACHKNB_RS02470"
                     /old_locus_tag="pgaptmp_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HGU4194073.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13960..15660
                     /gene="araB"
                     /locus_tag="ACHKNB_RS02475"
                     /old_locus_tag="pgaptmp_000495"
     CDS             13960..15660
                     /gene="araB"
                     /locus_tag="ACHKNB_RS02475"
                     /old_locus_tag="pgaptmp_000495"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HGU4194074.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDANGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15671..17173
                     /gene="araA"
                     /locus_tag="ACHKNB_RS02480"
                     /old_locus_tag="pgaptmp_000496"
     CDS             15671..17173
                     /gene="araA"
                     /locus_tag="ACHKNB_RS02480"
                     /old_locus_tag="pgaptmp_000496"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HGU4194075.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17342..18037
                     /gene="araD"
                     /locus_tag="ACHKNB_RS02485"
                     /old_locus_tag="pgaptmp_000497"
     CDS             17342..18037
                     /gene="araD"
                     /locus_tag="ACHKNB_RS02485"
                     /old_locus_tag="pgaptmp_000497"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4194076.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18112..20463
                     /gene="polB"
                     /locus_tag="ACHKNB_RS02490"
                     /old_locus_tag="pgaptmp_000498"
     CDS             18112..20463
                     /gene="polB"
                     /locus_tag="ACHKNB_RS02490"
                     /old_locus_tag="pgaptmp_000498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HGU4194077.1"
                     /translation="MAQAGFILTRRWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20628..23534
                     /gene="rapA"
                     /locus_tag="ACHKNB_RS02495"
                     /old_locus_tag="pgaptmp_000499"
     CDS             20628..23534
                     /gene="rapA"
                     /locus_tag="ACHKNB_RS02495"
                     /old_locus_tag="pgaptmp_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HGU4194078.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLGEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23546..24205
                     /gene="rluA"
                     /locus_tag="ACHKNB_RS02500"
                     /old_locus_tag="pgaptmp_000500"
     CDS             23546..24205
                     /gene="rluA"
                     /locus_tag="ACHKNB_RS02500"
                     /old_locus_tag="pgaptmp_000500"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HGU4194079.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24322..25137)
                     /gene="djlA"
                     /locus_tag="ACHKNB_RS02505"
                     /old_locus_tag="pgaptmp_000501"
     CDS             complement(24322..25137)
                     /gene="djlA"
                     /locus_tag="ACHKNB_RS02505"
                     /old_locus_tag="pgaptmp_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HGU4194080.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25392..27746
                     /gene="lptD"
                     /locus_tag="ACHKNB_RS02510"
                     /old_locus_tag="pgaptmp_000502"
     CDS             25392..27746
                     /gene="lptD"
                     /locus_tag="ACHKNB_RS02510"
                     /old_locus_tag="pgaptmp_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HGU4194081.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27799..29085
                     /gene="surA"
                     /locus_tag="ACHKNB_RS02515"
                     /old_locus_tag="pgaptmp_000503"
     CDS             27799..29085
                     /gene="surA"
                     /locus_tag="ACHKNB_RS02515"
                     /old_locus_tag="pgaptmp_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HGU4194082.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29085..30074
                     /gene="pdxA"
                     /locus_tag="ACHKNB_RS02520"
                     /old_locus_tag="pgaptmp_000504"
     CDS             29085..30074
                     /gene="pdxA"
                     /locus_tag="ACHKNB_RS02520"
                     /old_locus_tag="pgaptmp_000504"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HGU4194083.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30071..30892
                     /gene="rsmA"
                     /locus_tag="ACHKNB_RS02525"
                     /old_locus_tag="pgaptmp_000505"
     CDS             30071..30892
                     /gene="rsmA"
                     /locus_tag="ACHKNB_RS02525"
                     /old_locus_tag="pgaptmp_000505"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGU4194084.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30895..31272
                     /gene="apaG"
                     /locus_tag="ACHKNB_RS02530"
                     /old_locus_tag="pgaptmp_000506"
     CDS             30895..31272
                     /gene="apaG"
                     /locus_tag="ACHKNB_RS02530"
                     /old_locus_tag="pgaptmp_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HGU4194085.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31279..32127
                     /gene="apaH"
                     /locus_tag="ACHKNB_RS02535"
                     /old_locus_tag="pgaptmp_000507"
     CDS             31279..32127
                     /gene="apaH"
                     /locus_tag="ACHKNB_RS02535"
                     /old_locus_tag="pgaptmp_000507"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HGU4194086.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32152..32751
                     /locus_tag="ACHKNB_RS02540"
                     /old_locus_tag="pgaptmp_000508"
     CDS             32152..32751
                     /locus_tag="ACHKNB_RS02540"
                     /old_locus_tag="pgaptmp_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HGU4194087.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNILINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32787..33254
                     /locus_tag="ACHKNB_RS02545"
                     /old_locus_tag="pgaptmp_000509"
     CDS             32787..33254
                     /locus_tag="ACHKNB_RS02545"
                     /old_locus_tag="pgaptmp_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194088.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     SVAIDYGFFCSVSQQSIMNITRMAEGVWITESKTTQLIDIWQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(33353..33532)
                     /locus_tag="ACHKNB_RS02550"
                     /old_locus_tag="pgaptmp_000510"
     CDS             complement(33353..33532)
                     /locus_tag="ACHKNB_RS02550"
                     /old_locus_tag="pgaptmp_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HGU4194089.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33553..34032)
                     /gene="folA"
                     /locus_tag="ACHKNB_RS02555"
                     /old_locus_tag="pgaptmp_000511"
     CDS             complement(33553..34032)
                     /gene="folA"
                     /locus_tag="ACHKNB_RS02555"
                     /old_locus_tag="pgaptmp_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HGU4194090.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34225..36087)
                     /gene="kefC"
                     /locus_tag="ACHKNB_RS02560"
                     /old_locus_tag="pgaptmp_000512"
     CDS             complement(34225..36087)
                     /gene="kefC"
                     /locus_tag="ACHKNB_RS02560"
                     /old_locus_tag="pgaptmp_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HGU4194091.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAVTPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36080..36610)
                     /gene="kefF"
                     /locus_tag="ACHKNB_RS02565"
                     /old_locus_tag="pgaptmp_000513"
     CDS             complement(36080..36610)
                     /gene="kefF"
                     /locus_tag="ACHKNB_RS02565"
                     /old_locus_tag="pgaptmp_000513"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HGU4194092.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36718..38049)
                     /gene="yaaU"
                     /locus_tag="ACHKNB_RS02570"
                     /old_locus_tag="pgaptmp_000514"
     CDS             complement(36718..38049)
                     /gene="yaaU"
                     /locus_tag="ACHKNB_RS02570"
                     /old_locus_tag="pgaptmp_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194093.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38107..38394)
                     /gene="fixX"
                     /locus_tag="ACHKNB_RS02575"
                     /old_locus_tag="pgaptmp_000515"
     CDS             complement(38107..38394)
                     /gene="fixX"
                     /locus_tag="ACHKNB_RS02575"
                     /old_locus_tag="pgaptmp_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HGU4194094.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38391..39677)
                     /gene="fixC"
                     /locus_tag="ACHKNB_RS02580"
                     /old_locus_tag="pgaptmp_000516"
     CDS             complement(38391..39677)
                     /gene="fixC"
                     /locus_tag="ACHKNB_RS02580"
                     /old_locus_tag="pgaptmp_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4194095.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39728..40669)
                     /gene="fixB"
                     /locus_tag="ACHKNB_RS02585"
                     /old_locus_tag="pgaptmp_000517"
     CDS             complement(39728..40669)
                     /gene="fixB"
                     /locus_tag="ACHKNB_RS02585"
                     /old_locus_tag="pgaptmp_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4194096.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDTSTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40684..41454)
                     /gene="fixA"
                     /locus_tag="ACHKNB_RS02590"
                     /old_locus_tag="pgaptmp_000518"
     CDS             complement(40684..41454)
                     /gene="fixA"
                     /locus_tag="ACHKNB_RS02590"
                     /old_locus_tag="pgaptmp_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HGU4194097.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGSDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41928..43442
                     /gene="caiT"
                     /locus_tag="ACHKNB_RS02595"
                     /old_locus_tag="pgaptmp_000519"
     CDS             41928..43442
                     /gene="caiT"
                     /locus_tag="ACHKNB_RS02595"
                     /old_locus_tag="pgaptmp_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HGU4194098.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43473..44615
                     /gene="caiA"
                     /locus_tag="ACHKNB_RS02600"
                     /old_locus_tag="pgaptmp_000520"
     CDS             43473..44615
                     /gene="caiA"
                     /locus_tag="ACHKNB_RS02600"
                     /old_locus_tag="pgaptmp_000520"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HGU4194099.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44744..45961
                     /gene="caiB"
                     /locus_tag="ACHKNB_RS02605"
                     /old_locus_tag="pgaptmp_000521"
     CDS             44744..45961
                     /gene="caiB"
                     /locus_tag="ACHKNB_RS02605"
                     /old_locus_tag="pgaptmp_000521"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HGU4194100.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46035..47588
                     /gene="caiC"
                     /locus_tag="ACHKNB_RS02610"
                     /old_locus_tag="pgaptmp_000522"
     CDS             46035..47588
                     /gene="caiC"
                     /locus_tag="ACHKNB_RS02610"
                     /old_locus_tag="pgaptmp_000522"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HGU4194101.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47697..48482
                     /gene="caiD"
                     /locus_tag="ACHKNB_RS02615"
                     /old_locus_tag="pgaptmp_000523"
     CDS             47697..48482
                     /gene="caiD"
                     /locus_tag="ACHKNB_RS02615"
                     /old_locus_tag="pgaptmp_000523"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HGU4194102.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48488..49078
                     /gene="caiE"
                     /locus_tag="ACHKNB_RS02620"
                     /old_locus_tag="pgaptmp_000524"
     CDS             48488..49078
                     /gene="caiE"
                     /locus_tag="ACHKNB_RS02620"
                     /old_locus_tag="pgaptmp_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HGU4194103.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49164..49559)
                     /gene="caiF"
                     /locus_tag="ACHKNB_RS02625"
                     /old_locus_tag="pgaptmp_000525"
     CDS             complement(49164..49559)
                     /gene="caiF"
                     /locus_tag="ACHKNB_RS02625"
                     /old_locus_tag="pgaptmp_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HGU4194104.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49820..53041)
                     /gene="carB"
                     /locus_tag="ACHKNB_RS02630"
                     /old_locus_tag="pgaptmp_000526"
     CDS             complement(49820..53041)
                     /gene="carB"
                     /locus_tag="ACHKNB_RS02630"
                     /old_locus_tag="pgaptmp_000526"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGU4194105.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53059..54207)
                     /gene="carA"
                     /locus_tag="ACHKNB_RS02635"
                     /old_locus_tag="pgaptmp_000527"
     CDS             complement(53059..54207)
                     /gene="carA"
                     /locus_tag="ACHKNB_RS02635"
                     /old_locus_tag="pgaptmp_000527"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HGU4194106.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT        13192 a        14746 c        14255 g        12391 t
ORIGIN      
        1 aatcgcaaaa catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt
       61 tgtccccttt ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg
      121 aaaagcgtcg gtagttaagc agaaattaat atcgcttact ttaaccaccg cagcacaatt
      181 agctaatttt acggatgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga
      241 catccgtttt tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa
      301 tgactcacat cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta
      361 gacgagtgag cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat
      421 tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat
      481 gagccagcaa gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc
      541 aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga
      601 cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat
      661 cgcccgccag gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat
      721 cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc
      781 tacctcccca atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg
      841 cgctatctat atggtgaaac gcgcccgtaa ttacaccgat gatgttgaat tttcttgcga
      901 agatgccggg cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc
      961 cggtgccacc accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc
     1021 cggaatcatc agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt
     1081 acatacccac gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg
     1141 tgcacgccag gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct
     1201 ggaagaagtc atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat
     1261 taatcaccag gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat
     1321 cccggcaaac aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca
     1381 ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct
     1441 gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat
     1501 ggatgagatg gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct
     1561 gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat
     1621 cggtaagcag caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg
     1681 ctctaacgat atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga
     1741 agccgccaac ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga
     1801 ctataacgtc gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct
     1861 gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc
     1921 caccgatatt gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg
     1981 tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga
     2041 aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg tattggtccg
     2101 gaagtgatga cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt tgcgatgcgc
     2161 atcaccacca gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg
     2221 ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc
     2281 ggtccgaaat gggaacattt accaccagac cagcaaccag aacgcggcgc gctgttgcct
     2341 ttgcgtaagc acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg
     2401 gaagcattct gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg
     2461 cgcgaactga ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa
     2521 tatgaaaaag cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc
     2581 atcgcgtttg aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac
     2641 gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg
     2701 gatgtcgaac tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca
     2761 tcacagtttg acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc
     2821 gcaatgatca ctggctcgat ggggatgttg ccttccgcca gcctgaacga gcaaggtttt
     2881 ggtctgtatg aaccggcagg cggctcagca ccagatatcg caggcaaaaa catcgccaac
     2941 ccgattgcgc aaattctgtc gctggcactg ctgctgcgct acagcctgga tgccgatgat
     3001 gcggcttgcg ccattgaacg cgccattaac cgcgcattag aagaaggcat tcgcaccggg
     3061 gatttagccc gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc
     3121 tatgtggcag aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc
     3181 acgtagtgta cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc
     3241 atgaagtgac ctcaccgcag gcgtttgatg gtctgcgcgc ccacggtcgc ccggtacgtc
     3301 agccgggcaa aaccttcgcc actatggatc acaacgtctc tacccagacc aaagacatta
     3361 atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat
     3421 ttggcgtcga gctgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc
     3481 cggaacaggg cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca
     3541 cccacggcgc gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac
     3601 tggcaacaca aaccctgaaa cagggccgtg cgaagaccat gaaaattgaa gtccagggca
     3661 aagccgcgcc gggcattaca gcaaaagata tcgtgctggc aattatcggt aaaactggca
     3721 gcgcaggcgg caccgggcat gtggtggagt tttgcggcga agcaattcgt gatttaagca
     3781 tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat gggcgcaaaa gccggtctgg
     3841 ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg tttgcatgcg ccgaaaggca
     3901 aagatttcga cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt
     3961 tcgataccgt tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca
     4021 atccaggcca ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc
     4081 cggttgaacg cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggcattc
     4141 cgctgaccga agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg
     4201 aagatttacg cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg
     4261 cactggtggt tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata
     4321 aaatctttat tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga
     4381 tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg
     4441 aaggccgcca ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg
     4501 ctgctgtgac cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca
     4561 gagaaattta tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc
     4621 gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat
     4681 ctgtttaacg actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg
     4741 ctgaacttcc cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt
     4801 ggctcctcgc gtgagcacgc gccctgggca ttgaccgact acggttttaa agtggtgatt
     4861 gcgccgagtt ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg
     4921 aaattaagcg atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc
     4981 catttcgacg tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt
     5041 accatcgatg ccttccgccg ccactgcatg atgaacggtc tggacagtat tgggcttacc
     5101 ttgcagcacg acgacgccat tgccgcttat gaagcaaaac aacctgcgtt tatgaattaa
     5161 tccccttgcc cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt
     5221 aacctttgcg gttaaaaata atgcgacaac agaaataacc gcaattaccc agtagacagc
     5281 aaagtgcccc caactttgtg caattgctcc ctgaataacg ccagccagaa ttaccccggt
     5341 agaaatactg ttagtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg
     5401 aaaccatagc atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag
     5461 cgtcatcaac gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc
     5521 cgccactgct atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag
     5581 aatcattgct ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc
     5641 tggcaatcct aactcgctac tgatccacaa cggcatatca ataatgtaca tggtgttgca
     5701 ggtccacatt aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagccacc
     5761 ttgcattgat aaagcatttt ccgacggcag ttctacccgc gccacagacg gaagcataaa
     5821 tgcaatcaat accagactga gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa
     5881 gccgtaattc aacgccagca taaaggccaa cggtggaccg ataacccatg ccagagaaag
     5941 ctgcgcacgc atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcatattc
     6001 ccgcgccaga gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc
     6061 acaggtgata agcgtcagat aatgacgatt aaatgcaaac aatagcgcat tgccgatagc
     6121 catcaaacag caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc
     6181 caaccagagg cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca
     6241 gaaaggttgc gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc
     6301 cccggccacc cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg
     6361 gcgagccatc gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa
     6421 aaccagcagg tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg
     6481 caggcaagtc aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt
     6541 aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag
     6601 tgctgataaa actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta
     6661 atatagggaa aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct
     6721 cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca
     6781 acgctcaacg aactggcagc gttattgagc tgctcgcgtc gtcatatgcg caccctgctc
     6841 aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg tggtaaacgc
     6901 tcgcgtctga cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg
     6961 ctggagcagg atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg
     7021 caaatgctgg tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg
     7081 ctctactatc gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc
     7141 catattgccc ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg
     7201 gaagcagaca tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg
     7261 cgtccaggag tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct
     7321 ttaaaacgaa tcaatacgct gccgctctat tcgcatattg cggacattgt gtcgccgacg
     7381 ccctggacgc tggatatcca tctcacgcaa ccggaccgct ggttaccgtt actgctggga
     7441 caagttccgg cgatgatcct gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat
     7501 cccatcggca ccggtccgta tgcggtgatc cgcaacacca ccaatcaact gaaaattcag
     7561 gcattcgatg acttcttcgg ttaccgggca ttaatcgatg aagttaacgt ctgggttctg
     7621 ccggaaattg ccgacgagcc agctggaggt ctgatgctaa aaggtccaca gggtgaggaa
     7681 aaagagattg aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc
     7741 catcgcgggg cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat
     7801 ctggtctatt tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc
     7861 ccccgttggc accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc
     7921 accctgacct tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag
     7981 attctggcaa gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat
     8041 gaaggagaga tcgagagcga tatctggctt aacagcgcca actttacgct gccgctggat
     8101 ttttcgctgt tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac
     8161 tggcaagccg acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag
     8221 caactggtcg ccagcaaagc aatggtgccg cttatccacc actggctgat cattcagggg
     8281 caacgcagta tgcgcggcct gcgcatgaac accctcggct ggttcgattt taaatcagcg
     8341 tggtttgcgc caccggatcc atgattgctg gtagataaca aaatcactac actaacgccg
     8401 ttctcaacgg ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg
     8461 gataacgccg gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag
     8521 gtgcaaagtg ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc
     8581 taaacccgtt ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc
     8641 aaagatcaaa aaagcttttg aagccgactg taactgcgaa ctgaaactgg tggcgctgga
     8701 agatggcgtt tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt
     8761 ggtgctgggg ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa
     8821 aagcggtgtg gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt
     8881 cccgtttgat tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc
     8941 gcaaagcctg aaagaattgg ttgagagcga tcagaactgg cgggtgattt atcaggatcc
     9001 gcgcaccagt acgccggggc tgggcctgct gttgtggatg caaaaagtct atggcgatga
     9061 cgccccacaa gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag
     9121 cgaagcctac ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc
     9181 tccggcttat cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga
     9241 aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct
     9301 ggcgcaaaaa ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg
     9361 taactggatg tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa
     9421 accggccacc acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat
     9481 tagcgaatgg caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc
     9541 gccgccacgc tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg
     9601 ccgcagggta actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc
     9661 tccttctggc aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc
     9721 cgcgccctct atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg
     9781 accttgatcc tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag
     9841 ggctggctgg catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc
     9901 ctgcaaggca ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta
     9961 ctccaggcac tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg
    10021 cgcggctggc attttttccg cttcgtcgaa tggccgtggc tacggcgaca aatcccgccg
    10081 gttgccgcgc ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcgctgggc
    10141 ggcggtccgc aggcaactac tatcgagctg gctatttatc aggcgctgag ttacgactac
    10201 gatcctgccc gcgcggcgat gctggcgctg ctccagatgg tgtgttgcct tgggctggtg
    10261 ctgttgagtc agcgactaag taaggccatt gcgcccggta ccacgctgct gcaaggctgg
    10321 cgcgatccgg acgatcgtct gcatagccgc atttgcgaca cggtgttaat agtgctggcg
    10381 ctgctgctgt tgctgccacc gttgctggcg gtgatcgtcg atggggtaaa tcgccagttg
    10441 ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg
    10501 ctggcggcag gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa
    10561 cttcgggcgc ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc
    10621 ctcgccatgc cggggattgt gctggctacc ggcttctttt tactgctcaa caacaccatt
    10681 ggcctgccgc aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct
    10741 tatgcgctga aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg
    10801 tgtcagtcgc tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg
    10861 aaacgtccac tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc
    10921 gtagtggcgt tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa
    10981 attggctcct atcgcagcca ggacggtgcg gtcaccgcgt taattctgct actactctgt
    11041 tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat
    11101 cacctggctt tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca
    11161 ggtggcaatc ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgatcgccgg
    11221 ttttctgacg ccagccagcg gttcgctgac tatcgacggc gttgatcaca ccacgacgcc
    11281 gccgtcacgc cgtccggtgt cgatgctgtt tcaggaaaac aacctgttca gtcacctgac
    11341 ggtcgcgcag aacatcgggc tggggctaaa tccgggattg aaactgaacg cggcacagca
    11401 ggagaaaatg cacgctatcg cccgccagat ggggattgat aatttaatgg cgcggttacc
    11461 tggcgagctt tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga
    11521 acagccgatt ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga
    11581 gatgctgacg ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc
    11641 gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg
    11701 catcgcctgg cagggtaaga ccaatgagtt gttgagcggt aaggcgagtg cttcggcact
    11761 attggggatt acgggttagt atgcactgtc ggatgcggcg tgaacgcctt atccgaccta
    11821 cgggtagtgc gttttgtagg cctgataaga cgcggtcagc gtcgcatcag gcaacccgca
    11881 caagactatc tcctaacccc caaccacttt acgcaaaata tcgatataca ccggcatcaa
    11941 cgggtggcga attaacacca ccagcgccac cacgccgatg gcagaaatta acggcgtcag
    12001 ccacaacaaa cgaccacggg acaaataatg actcaaacgg tcagtcgctt taccactgcg
    12061 ccataaccgc cagcacagcc agccaccaac ccacaaaaac accgctgttg ccagcagcaa
    12121 ccatttaaac tcaccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat
    12181 ccctggcagg aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa
    12241 ttttgccacc ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc
    12301 aacaaaacga ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt
    12361 atcgagtaat gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg
    12421 ccagcccagc cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc ctgccagcca
    12481 ggcgtgccag aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac
    12541 cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac
    12601 cgccatcaat gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat
    12661 gtgcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
    12721 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
    12781 cacaaccggc tcggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
    12841 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
    12901 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
    12961 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg tgacaagcaa acatgctgtg
    13021 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
    13081 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
    13141 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
    13201 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
    13261 gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
    13321 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
    13381 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcataca aattctcgtc
    13441 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
    13501 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
    13561 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgt gcttcagcca
    13621 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
    13681 gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta
    13741 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
    13801 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
    13861 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
    13921 ctgtttctcc atacccgttt ttttggatgg agtgaaacga tggcgattgc aattggcctc
    13981 gattttggca gtgattctgt gcgagctttg gcggtggact gcgccagcgg tgaagagatc
    14041 gccaccagcg tagagtggta tccccgttgg caaaaagggc aattttgtga tgccccgaat
    14101 aaccagttcc gtcatcatcc gcgtgactac attgagtcaa tggaagcggc actgaaaacc
    14161 gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg tcgggattgg cgttgacaca
    14221 accggctcga cgcccgcacc gattgatgcc aacggtaacg tgctggcgct gcgcccggag
    14281 tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag accacactgc ggttgaagaa
    14341 gcggaagaga ttacccgttt gtgccacgcg ccgggcaacg ttgactactc ccgctacatt
    14401 ggcggtattt attccagcga atggttctgg gcaaaaatcc tgcatgtgac tcgccaggac
    14461 accgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg
    14521 ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataag
    14581 tcgctatggc acgaaagctg gggtggcttg ccgccagcca gtttctttga tgagctggac
    14641 ccgatcctca atcgccattt gccttccccg ctgttcactg acacctggac tgccgatatt
    14701 ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg
    14761 atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac
    14821 gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc
    14881 gttggcgagc gggcagtgaa aggtatttgc ggtcaggttg atggcagcgt ggtacctgga
    14941 tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc
    15001 gtactcggct ggccgctgga acagcttgcc gcccagcatc cggaactgaa agcgcaaatc
    15061 aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct
    15121 ctggatcacc tgccggtggt gctcgactgg tttaacggcc gccgcacacc gaacgctaac
    15181 caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacgctcc gctgctgttc
    15241 ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat
    15301 caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc
    15361 attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag
    15421 tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc
    15481 ccatcagccc agcaaaaaat ggccagcgcg gtagagaaaa ccctgcaacc gcgcagcgaa
    15541 caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa
    15601 caacactatc ttccaacttc cgccccggca caggcagccc aggccgttgc gactctataa
    15661 ggacacgata atgacgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca
    15721 tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc
    15781 gctgaatacg gaagcgaaac tgccctgcaa actagtgttg aaaccgctgg gcaccacgcc
    15841 ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt
    15901 ggtgtggctg cacactttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa
    15961 caaaccgttg ctgcaattcc acacccagtt taacgcggcg ctgccgtggg acagtatcga
    16021 tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg
    16081 cgcgcgtatg cgtcagcaac atgccgtggt taccggtcac tggcaggata aacaagccca
    16141 tgagcgtatc ggctcctgga tgcgccaggc ggtctctaaa caggataccc gtcatctgaa
    16201 agtctgccgt tttggcgata acatgcgtga agtagcggtc accgatggtg ataaagttgc
    16261 cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt
    16321 ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta
    16381 caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc
    16441 gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac
    16501 cacctttgaa gatttgcacg gtctgaaaca gcttccaggt ctggccgtac agcgtctgat
    16561 gcagcagggt tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat
    16621 catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta
    16681 tcacttcgag aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc
    16741 gattgccgta gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga
    16801 cgatcctgcc cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat
    16861 tgatctcggc gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca
    16921 ctccctgccg aaactgccgg tggcgaatgc gctgtggaaa gcgcaaccgg atctgccaac
    16981 tgcttccgaa gcgtggatcc ttgctggtgg cgcgcaccat accgtcttca gccatgcgct
    17041 gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga
    17101 taacgacacc cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg
    17161 atttcgtcgc taagtagcca catcaggtat gtaacgcctg atgcgacgct gacgcgtctt
    17221 atctggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattgttt
    17281 tccccctcgc ccctttgggg agagggccgg ggtgagggga aacgacctct caggagtcaa
    17341 catgttagaa gatctcaaac gccaggtatt agaagccaac ctggcgctgc caaaacacaa
    17401 cctggtcacg ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg gcgtctttgt
    17461 gatcaaacct tccggcgtcg attacagcgt catgaccgct gacgatatgg tcgtggttag
    17521 catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc tcctccgaca cgccaactca
    17581 ccggctgctc tatcaggcat tcccctccat tggcggcatt gtgcatacgc actcgcgcca
    17641 cgccaccatc tgggcgcagt cgggtcagtc gattccagca atcggcacca cccacgccga
    17701 ctatttctac ggcaccattc cctgcacccg caaaatgacc gacgcagaaa tcaacggcga
    17761 atatgagtgg gaaaccggta acgtcatcgt agaaaccttt gaaaaacagg gtatcgatgc
    17821 agcgcaaatg cccggcgttc tggtccattc ccacggcccg tttgcatggg gcaaaaatgc
    17881 cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg ggatattctg
    17941 ccgtcagtta gcgccgcagt taccggatat gcagcaaacg ctgctggata aacactatct
    18001 gcgtaagcat ggcgcgaagg catattacgg gcagtaatga ctgtataaaa ccacagccaa
    18061 tcaaacgaaa ccaggctata ctcaagcctg gttttttgat ggattttcag cgtggcgcag
    18121 gcaggtttta tcttaacccg acgctggcgg gacaccccgc aagggacaga agtctccttc
    18181 tggctggcga cggacaacgg gccgttgcag gttacgcttg caccgcaaga atccgtggcg
    18241 tttattcccg ccgatcaggt tccccgcgct cagcatattt tgcagggtga acaaggcttt
    18301 cgcctgacac cgctggcgtt aaaggatttt caccgccagc cggtgtatgg cctttactgt
    18361 cgcgcccatc gccaattgat gaattacgaa aagcgcctgc gtgaaggtgg cgttaccgtc
    18421 tacgaggccg atgtgcgccc gccagaacgc tatctgatgg agcggtttat cacctcaccg
    18481 gtgtgggtcg agggtgatat gcacaatggc actatcgtta atgcccgtct gaaaccgcat
    18541 cccgactatc gtccgccgct caagtgggtt tctatagata ttgaaaccac ccgccacggt
    18601 gagctgtact gcatcggcct ggaaggctgc gggcagcgca tcgtttatat gctggggccg
    18661 gagaatggcg acgcctcctc gcttgatttc gaactggaat acgtcgccag ccgcccgctg
    18721 ctgctggaaa aactcaacgc ctggtttgca aaccacgatc ccgatgtgat catcggctgg
    18781 aacgtggtgc agttcgatct gcgaatgctg caaaaacatg ccgagcgtta ccgtcttccg
    18841 ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg agcacggctt taaaaacggc
    18901 gtcttttttg cccaggctaa aggtcggcta attatcgacg gtatcgaggc gctgaaatcc
    18961 gcgttctgga atttctcttc attctcgctg gaaactgtcg ctcaggagct attaggcgaa
    19021 ggaaaatcta tcgataaccc gtgggatcga atggacgaaa ttgaccgccg tttcgccgaa
    19081 gataaacctg cgctggcaac ttataacctg aaagattgcg agctggtgac gcagatcttc
    19141 cacaaaactg aaatcatgcc atttttactc gaacgagcaa cggtgaacgg cctgccggtg
    19201 gatcggcacg gcggttcggt ggcagcgttt ggtcatctct attttccgcg aatgcatcgc
    19261 gctggttatg tcgcgcctaa tctcggcgaa gtgccaccgc acgccagccc tggcggctac
    19321 gtgatggatt cacggccagg gctttatgat tcagtgctgg tgctggacta taaaagcctg
    19381 tacccgtcga tcatccgcac ctttctgatt gatcccgtcg ggctggttga aggcatggcg
    19441 caacctgatc cagagcacag taccgaaggt tttctcgatg cctggttctc acgggagaaa
    19501 cattgcctgc cggagattgt gactaacatc tggcacgggc gcgatgaagc caaacgtcag
    19561 ggtaataagc cgctttcgca ggcgctgaag atcattatga atgcctttta tggcgtgctc
    19621 ggcaccaccg cctgccgctt cttcgatccg cgcctggcat cgtcgatcac catgcgtggt
    19681 catcagatca tgcggcagac caaagcattg attgaagcgc agggctacga cgttatctac
    19741 ggagataccg actcgacttt tgtctggcta aaaggtgcac attcggaaga agaggcggcg
    19801 aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt gggcggaaac gctgcaaaaa
    19861 caacggctga ccagcgcatt agaactggag tatgaaactc atttctgccg ttttctgatg
    19921 ccaaccattc gcggagccga taccggcagt aaaaagcgct acgccgggct gattcaggag
    19981 ggcgataaac agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga ctggacgccg
    20041 ctggcccagc agtttcagca ggagctatac ctgcgcatct tccgcaacga gccatatcag
    20101 gaatatgtac gcgaaaccat cgacaaactg atggcgggtg aactggatgc gcgactggtt
    20161 taccgtaaac gccttcgccg tccgctgagc gagtatcagc gtaatgtgcc gcctcatgta
    20221 cgcgccgctc gccttgccga tgaagaaaac caaaagcgcg gtcgcccctt gcaatatcag
    20281 aatcgcggca ccattaagta cgtatggacc accaacggcc cggagccgct ggactaccaa
    20341 cgttcaccac tggattacga acactatctg acccgccagc tacaacccgt ggcggaggga
    20401 atactccctt ttattgagga taattttgct acacttatga ccgggcaact tgggctattt
    20461 tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg tgacgaatgc tttgccatcc
    20521 agtaccatag cgccctttcc attcctggac ctgaataaca ccactacctc ataagcacgg
    20581 tagcgggtgg ttattgcctg caattaaaga tatagagccg aacacatatg ccttttacac
    20641 ttggtcaacg ctggatcagc gatacagaaa gcgaattggg acttggaacc gttgtcgcgg
    20701 tggatgcgcg aactgtcact ttacttttcc catctactgg tgaaaaccgt ctgtacgcac
    20761 gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg tgatactatt accagccatg
    20821 acggctggca gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg acctatatcg
    20881 gtactcgcct ggatactgaa gagtccggcg tagccctgcg tgaagttttc cttgatagca
    20941 aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg gcagattgac cgtatggacc
    21001 gctttgcgct gcgttatcgc gcgcgtaagt attccagcga acagttccgt atgccgtaca
    21061 gcggcctgcg cggtcagcgt accagcctga ttccgcatca gctcaacatc gctcatgatg
    21121 ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga agtgggttta gggaaaacca
    21181 ttgaagccgg gatgatcctg catcagcaac tgctctctgg cgctgctgaa cgtgtgctaa
    21241 ttatcgtccc ggaaacctta cagcatcagt ggctggtaga aatgctgcgc cgtttcaacc
    21301 tgcgctttgc gctatttgat gatgagcgtt atgccgaagc tcagcacgat gcttacaacc
    21361 cgtttgacac cgaacagctg gtgatttgct cgctggattt tgcccgtcgt agcaaacagc
    21421 gcctggaaca tctcggtgaa gccgaatggg acctgctggt ggtcgatgaa gcgcatcacc
    21481 tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa ctggcagagc
    21541 acgtgccggg cgttctgctg ctgaccgcga ccccggaaca gctggggatg gaaagccact
    21601 tcgcccgtct gcgtctgctg gacccgaacc gtttccacga ttttgcgcag ttcgttgaag
    21661 agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat gctgctggca ggtaacaaac
    21721 tgagcaatga cgaactgaac atgctcggcg agatgatcgg cgagcaggat atcgagccgc
    21781 tgttgcaggc agcaaacagc gacagcgaag atgcccagag cgcccgtcag gagctggttt
    21841 cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg cgtaacggtg
    21901 tgaaaggatt cccgaaacgc gagctgcaca ccattaaact gccgctaccg acgcagtatc
    21961 agacggctat taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa gatcgtgctc
    22021 gcgatatgct ctacccggag cgtatttatc aggaatttga aggtgataac gccacctggt
    22081 ggaacttcga tccgcgcgtt gagtggctga tgggctacct gaccagccat cgctctcaga
    22141 aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca actggagcag gtactgcgcg
    22201 aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat gtcgattatc gaacgtgacc
    22261 gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg tgctcagaaa
    22321 tcggttctga aggacgtaac ttccagttcg ccagccacat ggtgatgttt gacctgccat
    22381 tcaacccgga tctactggag cagcgtattg gtcgtctgga tcgtatcggc caggcgcacg
    22441 atattcagat ccatgtgcct tatctggaga aaaccgctca gtcggtgctg gtgcgctggt
    22501 atcacgaagg tctggatgca tttgagcaca cctgcccgac cggacgcact atttacgata
    22561 gcgtatacaa cgatctgatt aactatctgg cttcaccgga tcaaaccgaa ggctttgacg
    22621 atctgatcaa aaactgccgc gagcaacatg aagcgctgaa agcacaactg gaacagggtc
    22681 gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa agcccaggca ctggcagaaa
    22741 gcattgaaga gcaggatgac gataccaacc tgatcgcctt cgccatgaac ctgttcgata
    22801 ttatcggtat caatcaggac gatcgcggcg acaacatgat cgtgctgacg ccgtccgatc
    22861 atatgctggt gccggacttc cctggcctgt cggaagatgg catcaccatc acctttgatc
    22921 gtgaagtggc gctggcgcgt gaagatgctc agtttattac ctgggaacat ccgctgatcc
    22981 gcaacggtct ggatctgatc ctttctggcg ataccggtag cagcacgatt tcactgttaa
    23041 aaaacaaagc gttgccggta ggtacgctgt tggtggaact gatttacgtg gtcgaagccc
    23101 aggcgccgaa gcagttgcag ctcaaccgct tcctgccacc gacgccggta cgtatgctgc
    23161 tggataaaaa cggcaacaac ctggcggccc aggtggagtt tgaaaccttt aaccgccagc
    23221 ttaacgcggt taaccgtcac accggcagca aactggttaa cgccgtgcag caggatgttc
    23281 acgctatcct tcagctgggt gaagcgcaga tcgagaaatc tgcccgtgca ttgattgatg
    23341 ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg gaagcgctgc
    23401 gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc cattgagagc aaccgtcagc
    23461 aggtaatgga aagcctggat caggcaggct ggcgtctgga tgccctgcgt ttgatcgttg
    23521 taacgcatca gtaacggagc cgaaaatggg gatggaaaac tacaatccac cgcaggaacc
    23581 ctggttggtt atcctgtatc aggatgacca cattatggtg gttaacaagc cgagcggttt
    23641 gttgtctgtg ccgggtcgtc tggaagagca caaagatagc gtgatgacgc gcattcagcg
    23701 tgattatccg caggcagaat cggtgcatcg tctggatatg gctaccagcg gcgtgattgt
    23761 agtggcgctg accaaagccg cggagcggga gttaaaacgc cagttccgcg agcgcgagcc
    23821 gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc cccgcagaag gtctggtgga
    23881 tctgccgctg atttgcgact ggccaaaccg cccgaaacag aaagtctgtt acgaaacggg
    23941 taaacctgcg cagacggaat atgaagtggt ggagtatgcg gcggataaca cggcaagagt
    24001 ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc tggcgctggg
    24061 gcatccgatt ctcggcgatc gtttttatgc atcaccagaa gcgagagcga tggcaccgcg
    24121 tttgttgcta catgcagaga tgttgacgat tacccatccg gcgtatggca atagtatgac
    24181 gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc agttcggtag gcggataagg
    24241 cgttcacgcc gcatccggca attgtgcgcc gattgcctga tgcgacgcgg gccgcgtctt
    24301 atcaggccta cagtttaaaa cttatttaaa ccctttctgc tgctttatca gctcatatgc
    24361 ctgctgaatt tcctgcgctt tctgcttcgc catctccatc atctcaggcg gcaaaccttt
    24421 cgccaccagc ttatcgggat ggtgttcact catcagctta cggtaggcac gtttgatggt
    24481 ggtcgcatca tccgtcggct tcacgcccag cacattacag gcatcttcca gcgtcgggcc
    24541 acgctgcgct tgctgccagt taccaccgcc agattgctgc tgataaccgc cgccaaactg
    24601 tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga tccctaattc
    24661 ttcagcaatg acatacagca ccgcccgttc attcgggtgc agtgaaccat cagcaaacgc
    24721 cgcctgaatc tggatctcca gaaacatacg aattaagtca aaacgaccaa agcagacact
    24781 gcgaaactgg cgcatctttt cgcgcagcgg gtaattgtct gattttccca cccggaacgc
    24841 attttgcgcc gcagtacggg aagcgccatg aagattcatt cggtccatca actggctggc
    24901 gatatgaata tcagcctccg tgacgcgacc tttggatttg gttaaatgcc ccatcacttc
    24961 aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc acgccatttt
    25021 acggctacgg gctttatcaa acatatggcc aattaacagg cctaacacta cgccccaaaa
    25081 gccgccgccc atcagtaagg ccacggccac gccaatgatt tttccccaat actgcatata
    25141 ttccccaaat cgacacacgg atatcagggc tatctcccac aatataaagg tgcttttacc
    25201 gttttccggc tgcggtcaat tgtgggacat cgcctataat ttgcattatc atacctgtca
    25261 ttcaatgccg tgcctaacac cactgacgct attcggacag gattaacact agcgtagaga
    25321 tgacgagtac gttagtctct gaccgtttgt cacgcgcaac gttaccgatg atggaacaat
    25381 aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc caccatgatt gccaccgccc
    25441 tttatagtca acagggactg gcagccgacc tcgcctcaca gtgcatgttg ggcgtgccaa
    25501 gctatgaccg tcctctggta cagggcgata ccaatgactt acccgtgact atcaatgctg
    25561 accacgcgaa aggggactac ccggatgacg ccgtgtttac tggcagcgtg gatatcatgc
    25621 agggtaacag ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag gcaccaggac
    25681 aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac gataaccagg
    25741 tgatcctcaa agggccgaaa ggctgggcga atctgaacac caaagatacc aacgtctggg
    25801 aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg atgaaacaac
    25861 gtggcgaaaa ccgctatacc attctggata acggtagctt tacctcctgt ctgccgggtt
    25921 ctgacacctg gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa caagttgcgg
    25981 agatctggaa cgcccgcttt aaggtgggtc cggtaccgat cttttatagc ccctatttgc
    26041 agttgccggt gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc aagtacacca
    26101 ccaccaacta ctttgagttc tacctgccat attactggaa catcgcgcca aatatggatg
    26161 ccaccatcac gccgcattat atgcatcgtc gtggcaacat catgtgggag aacgaattcc
    26221 gctacctctc ccaggcgggc gctggcttga tggaactgga ctatctgcct tcagataaag
    26281 tctatgaaga tgaacacccg aacgatgaca gttcacgtcg ttggttattc tactggaacc
    26341 actccggggt catggatcaa gtgtggcgtt tcaacgtcga ctacaccaag gtcagcgatc
    26401 ctagctactt caatgatttc gataacaagt acggttccag tactgacggc tacgcaacgc
    26461 aaaaattcag cgttggctat gcggtgcaaa acttcaatgc caccgtttca accaagcagt
    26521 tccaggtttt cagcgaacag aacaccagta gctactcggc agagccgcag ttagacgtta
    26581 attactacca gaatgatgtt ggtccgtttg atacgcgtat ttacggccag gcagtgcact
    26641 ttgttaacac cagagacgac atgcctgaag caacccgtgt tcacctggaa ccgaccatca
    26701 atttgccgct ctctaataac tggggcagca tcaataccga agcgaagttg ctggcaaccc
    26761 attatcagca aaccaatctt gactggtata actccagaaa cacgaccaag ctggacgaat
    26821 ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa aatggtcttt gaacgcgata
    26881 tggaaatgct ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag tatttgtacg
    26941 tgccgtatcg cgatcagagc gacatctata actacgactc gtctctgctg caatctgact
    27001 actctggcct gttccgggac cggacttacg gcggtcttga ccgtattgcc tccgctaacc
    27061 aggtgacgac cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa cgttttaata
    27121 tttccgttgg tcaaatctac tatttcacgg agtctcgcac tggcgatgac aacataacat
    27181 gggagaatga cgacaaaacg ggttcactgg tgtgggcagg cgatacttac tggcgtatct
    27241 ccgagcgttg gggattgcgt ggcgggattc agtacgatac acgtctggat aacgtagcga
    27301 ccagtaactc cagcattgaa taccgtcggg atgaagaccg tctggtacag ctgaattacc
    27361 gttacgccag cccggaatat attcaggcta cgctgcctaa gtactattcc actgctgagc
    27421 aatataagaa tggtatttcg caggtaggtg ctgtcgccag ctggccaatt gccgatcgtt
    27481 ggtccattgt tggggcctac tactacgaca ccaatgcgaa caagcaagcc gactctatgt
    27541 taggtgtgca atacagctcc tgctgctatg caattcgcgt cggttacgag cggaagctga
    27601 acggttggga taacgataaa caacatgcgg tatatgacaa cgcaatcggc tttaacatcg
    27661 aacttcgcgg cctgagctcc aactacggtc tgggtacgca agagatgctg cgttcgaaca
    27721 ttctgccgta tcaaaacact ttgtgatttg ttgatttacc acgtaatccg cagtgcggtt
    27781 aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat cgccatgatc
    27841 gcgaatacca gtttcgctgc cccccaggta gtcgataaag tcgcagccgt cgtcaataac
    27901 ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt cggtaaaact gaacgctgct
    27961 caggcaaggc agcaacttcc tgatgacgcg acgctgcgcc accaaatcat ggaacgtttg
    28021 atcatggatc aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat ctccgatgag
    28081 cagctggatc aggcgattgc taacatcgcg aaacagaaca acatgacgct ggatcagatg
    28141 cgcagccgtc tggcttacga tggtctgaac tacaacactt atcgtaacca gatccgcaaa
    28201 gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac catcctgccg
    28261 caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa acgacgccag cactgagctg
    28321 aacctgagcc acatcctgat cccgctgccg gaaaacccga cctctgatca ggtgaacgaa
    28381 gcggaaagcc aggcgcgtgc cattgtcgat caggcgcgta acggcgctga tttcggtaag
    28441 ttggcgattg ctcattctgc cgaccagcag gcgctgaacg gcggccagat gggctggggc
    28501 cgtattcagg agttgccggg gatcttcgcc caggcattaa gcaccgcgaa gaaaggcgac
    28561 attgttggcc cgattcgttc cggcgttggc ttccatattc tgaaagttaa cgacctgcgc
    28621 ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc gccatattct gctgaaaccg
    28681 tcgccgatca tgactgacga acaggcacgc gtgaaactgg aacagattgc tgctgatatc
    28741 aagagtggta aaacgacttt tgctgccgca gcgaaagagt tctctcagga tccaggctcc
    28801 gctaaccagg gcggtgatct cggctgggct acaccagata ttttcgatcc ggccttccgt
    28861 gacgccctga ctcgcctgaa caaaggtcaa atgagtgcac cggttcactc ttcattcggc
    28921 tggcatttaa tcgaactgct ggatacccgt aatgtcgata aaaccgacgc tgcgcagaaa
    28981 gatcgtgcat accgcatgct gatgaaccgt aagttctcgg aagaagcagc aagctggatg
    29041 caggaacaac gtgccagcgc ctacgttaaa atcctgagca actaatggtt aaaacccaac
    29101 gtgttgtgat cactcccggc gagcccgccg ggattggccc ggacttagtt gtccagcttg
    29161 cacagcgtga gtggccggtc gaactggttg tttgtgccga taccactctc cttaccgacc
    29221 gggcagcgat gctcggtttg ccgctcaccc tccgccctta ttcccccaac tcccctgcac
    29281 aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa tctgtcactg
    29341 cggggcagtt aggggttgaa aatgggcatt acgtggtgga gacgctggcg cgagcgtgtg
    29401 atggctgtct gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat aaaggcgtca
    29461 ttaacgacgc aggtattccg tttactggtc ataccgagtt tttcgaagag cgttcgcagg
    29521 cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg gcaacgacgc
    29581 atttaccgct gcgcgatatc gcagatgcta tcacccctgc acttttgcac gaagtgattg
    29641 ctattttgca tcacgatttg cggacaaaat ttggtattgc cgaaccgcgc attctggtct
    29701 gcggactgaa tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag atagacacca
    29761 ttattccggt gctcgacgag ctgcgggcgc aggggatgaa actcaacggg ccgctgcctg
    29821 ccgataccct gtttcagccg aaatatctcg ataacgccga cgccgtgctg gcgatgtacc
    29881 acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg aacattacgc
    29941 tgggcctgcc ctttattcgc acatcagtgg accacggcac cgcgcttgaa ctggcgggac
    30001 gtggtaaagc cgatgtcggc agttttatta cggcgcttaa tctcgccatc aaaatgattg
    30061 ttaacaccca atgaataatc gagtccacca gggccactta gcccgtaaac gcttcgggca
    30121 aaactttctc aacgatcagt tcgtgatcga cagtattgtt tctgccatta acccgcaaaa
    30181 gggccaggcg atggtcgaaa tcggccccgg tctggcggca ttgaccgaac cggtcggcga
    30241 acgtctggac cagctgacgg tcatcgaact tgaccgcgat ctggcggcac gtctgcaaac
    30301 gcatccattc ttaggcccga aactgacgat ttatcagcag gatgcgatga cctttaactt
    30361 tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc tgccttataa
    30421 catctccacg ccgttgatgt tccatctgtt tagctatact gatgccattg ccgacatgca
    30481 ctttatgttg caaaaagagg tggtgaatcg tctggttgca ggaccgaaca gcaaagcgta
    30541 tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac tggaagttcc
    30601 gccgtcagcc tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc tggttcctca
    30661 tgcaacgatg cctcacccgg ttaaagatgt tcgtgtgttg agccgcatca ccaccgaagc
    30721 ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta gcgtcgaggt
    30781 gttaacggga atggggatcg acccggcgat gcgagcggaa aatatctctg tcgcgcaata
    30841 ttgccagatg gcgaactatc tggcggagaa cgcgcctttg caggagagtt aacgatgatc
    30901 aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca atcttcacct
    30961 gataatgaac gttacgtttt tgcttatacc gtaaccatac gcaatctggg gcgagcgcca
    31021 gtgcagttgt tggggcgtta ctggctgatc accaatggca atggccgtga aaccgaagtc
    31081 cagggcgaag gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga gtaccagtac
    31141 accagcggtg caatcattga aaccccgctg ggcaccatgc agggtcacta cgaaatgatc
    31201 gatgaaaatg gcgtcccttt cagcatcgac attcccgtat tccgactcgc cgttcccaca
    31261 ctcattcatt aaaagaatat ggcgacatac cttattggcg acgttcatgg ttgttacgat
    31321 gaactgatcg cattgctgca taaagtagaa tttacccctg ggaaagatac cctctggctg
    31381 acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg ttctgcgcta tgtgaaatcc
    31441 ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct ggcggtattt
    31501 gccgggatca gccgcaataa accgaaagat cgcctgacac cgctgctgga agcgccggat
    31561 gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc tgcaaatcga cgaagagaaa
    31621 aagttggtga tggcccacgc agggatcacg ccgcagtggg atctgcagac cgccaaagag
    31681 tgcgcgcgcg atgtagaagc ggtgctgtcg agcgactcct atcccttctt ccttgatgcc
    31741 atgtacggcg atatgccaaa taactggtca ccggaattgc gggggctggg aagactgcgt
    31801 tttatcacca acgcctttac ccgtatgcgt ttttgctttc caaacggtca actggatatg
    31861 tacagcaaag aatcgccgga agaagcccct gccccactga aaccgtggtt tgcgattcct
    31921 ggccctgtcg ctgaagaata cagcatcgcc tttggtcact gggcatcgct ggagggcaaa
    31981 ggtacgccgg aaggtatata cgcgctggat accggctgct gctggggtgg tacattaacc
    32041 tgcctgcgct gggaagataa acagtatttt gtccagccgt cgaaccggca taaggatttg
    32101 ggcgagggag aggcggtcgc gtcttaacga attaccattc agggaggaat aatggctgac
    32161 tattttgaaa tacgtgctga ttacaatcag cacaccatca cggtttatca ggcttacaat
    32221 gatgcaatcg ctgacgtcgc cgtacgtgac gggcgatttg gcgcatcttt ttcattcaac
    32281 cgcatgacct ggattaaacc ctcttttatg tggatgatgg agcgtagcaa ctggggactg
    32341 aaaaaagatc aacaacacat tctcgcaatc cgaatcaaac gcaccttctt tgatacattg
    32401 ctggaacaag cggtgctaac cacgccggaa gcccatgtgt atccacatgc aggcatttgg
    32461 gaaacgttat ttgcacaagc aaatgtgtat gttcagtggg atcccgaacg cagtataaat
    32521 ggtaaaaaac tggagcaccg atcattacag cttggtattt cccgtaactt gatcagtcaa
    32581 tttaacgaag acgccattgt tgccattgat gatctgactc cgctggtgcg aaagtgtcat
    32641 aacattttga taaacggtaa aacaacacag gcgaaatcct tcctgccgcc agaaaaaatt
    32701 taccctgtta gtgcagctgc gagaaaggct ttgggaatga aaaattcctg agacaaatac
    32761 gcgcctatta agcggaaaat atctaagtgc gacaaatcaa cagcgggctt aataccagag
    32821 aaattattct ggcaatgatt gacgaagcag atgaaccccc atttgctgag cagcggacct
    32881 ggcatcgcag gcgaatcgct tttctatgtg attgcatcag cgttgctatc gactacggct
    32941 tcttctgttc tgtctctcag cagtcaataa tgaacataac caggatggct gaaggtgtat
    33001 ggataacaga aagtaaaacg acccaactca ttgatatttg gcaacaactt aagcagcttt
    33061 atccgccaga aactattccc tacgtccata agttacccac taaggaatta cttgaactgc
    33121 tactttctag tcctgatgat cttcgaataa ttgaaattcc tgacatcatc ggtgatgttt
    33181 ttgatgacca aacctggatt cggttattca aaaaacactt tgctgatatt atccaactac
    33241 gatgtgatgt gtaacaatcc aaaatatatc gccagacgat agaccaaaaa gaaagtattt
    33301 agcagatact caacattctg acttctgtac agaaatagca gagtttaaaa acttaaaact
    33361 tccggtactc atcagagaaa catcctgttt cttctgcaaa ttcatttaat gccgagatgg
    33421 catcctcgtt tacttcattc cactgtagag tggcttgaag gtttagacct acgcaatgag
    33481 cgcgactcac aaaatcgcgc tcactggtaa aatcaaacgt cttatcgttc aatttgtagt
    33541 cctggcaaaa aattaccgcc gctccagaat ctcaaagcaa tagctatgag agttctgcgc
    33601 atcggcgtcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
    33661 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg
    33721 caggaactgc tcataaacgc gaccaccgcc aatcaccatg atttctggta cgtcaccaca
    33781 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg
    33841 actgctgagg ataatatttt tgcgtcctgg cagcggacga ccgatggatt cccaggtatg
    33901 gcggcccata attacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    33961 caggttccac ggcatggcgt tttccatgcc gataacacga tctaccgcta acgccgcaat
    34021 cagactaatc attgatttat cctggatata aaaaattgtc gccactatac gtaaagcata
    34081 aaccgtcgtc gactggcgca aggatgatgt tgagtaaaat tttacgttct gctggcgagt
    34141 ccacgctctc ccactggact cgccacatta caatgaaaca aaaacaaaca gttagctgta
    34201 aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt catccgccat
    34261 gttgccggta tgtttccctt cttcggttcc ctgccagcca tgtcgttgaa ttaatgacag
    34321 atgttcgcgg tcttcggtaa tgatctcact taacatcgcg ctggtgcgtt tatagaccgc
    34381 tgcgcgggct ttggtgtcgt tctcaaccat tgccatctct tccaccatct gaatattaaa
    34441 gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag
    34501 tgccagacgc ccggttttca gcgcaccttc gaaggtttca cgctccggct tttcaacgcc
    34561 tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg
    34621 cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatggc
    34681 gttaatcagc acttccgctt tcgccgctcc ggcagactcc agtaaatcca tccgcgtggc
    34741 atcgccataa aacactttca taccaaattt acgcaaggtt tcgatatggt ccgggtcgtg
    34801 atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtga tctgcccgaa
    34861 acgaccgaat ccggcgataa tcacgcgtgg ctgttcttcg tcgatctcat cggcttcacg
    34921 cgcttcctca gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttac
    34981 tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt
    35041 cgccatctgc gccgcaccaa ataccacaaa ggcaaactca ctaccctgcc ctaacaacac
    35101 cgcaaaccaa cgacgctgtt tatttggcac ttgcaacggt cgggcaatca gccacagcat
    35161 ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaag
    35221 cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc
    35281 tttaaatggt tcgatatcgc tctccagcgc atgacggtat tccgagcttg ccagcagtac
    35341 gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa
    35401 gccaaacacg aggaataacg ccacggcact aaacacttcc cgcaagccag agcgggctac
    35461 aaaacgcagc gccggacgcg tgacatagcg ccccagcaat accaccagca ccagcgcacc
    35521 cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgttgc
    35581 cagtagcgga atcatcgcca ccagcgggat cgccgcgata tcctggaaca gcagcaccgc
    35641 aaaggcactg cgacccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc
    35701 aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct gccagcgcag
    35761 cccaagtaac atgcagaaca gccccagcag gccaccgcaa atcaccatct gcaatgcgcc
    35821 gccgccgaac actgccgcac gcagcttcca tagcctttgt ggatcgagtt cgaggccgat
    35881 aataaacagc atcagcacca cgccaatctc ggcaaagtgc aggatagatt cggcatcggt
    35941 caccagtcgc agcccccacg ggccaataat gcagccggca atcaggtagc caagtaccga
    36001 tcccagacca agacgtaccg caatgggtac aatcagcgcc gccgatccga ggtagatcag
    36061 cgcctgaacc agcgtatggc tatccatgat gggcctcctg ccattccagc agacgttgct
    36121 tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg
    36181 caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca
    36241 gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca
    36301 ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt
    36361 gcgagaaaac tttatcgatc caaagtttga ggagcggtgg aatgctgtac cactgcatcg
    36421 ggtgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg gcaatatcga
    36481 tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtccttg
    36541 cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatta
    36601 taagaatcat aggtagcccg cgtgattacg cctttgtttt cccacaaagt gtagtcatat
    36661 attcacgcgg cttatagtca ataatattga ttcagaaaag agagaaatct gaacaattta
    36721 ccccattctc tgcccgcgga tcgtcatatt gctggtttgc gccagtgaca tccctcgagt
    36781 ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag
    36841 catcgtgtta ctgataccgt aattattgat aaagatcggc agtgcccagg tcgaaacaat
    36901 ggtgccaata cggcttaagg acataatcac gcccacggca gaggcgcgga tatctgtcgg
    36961 gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata
    37021 caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc ccaaaaccgc
    37081 cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt
    37141 tagccacagc atcggcggaa tacagccgag cataaagaac aggctaatca ccacgttccc
    37201 cagtgccgcg tttttgccaa cccccaatcc caacaaacca acgatttgcg ggccaaaggt
    37261 gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa
    37321 aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcatc
    37381 gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt ttactcgccc
    37441 tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca aaatcaacaa
    37501 acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat cataaagcca
    37561 gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga taaagctgat
    37621 ggaaaacgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg cgatgggata
    37681 atctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga caggggatga
    37741 gacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt
    37801 gcgccgtccg actttatcgg agatatagcc aaacagcgat gtgccaacga acagcccggc
    37861 gagcgttccc gcacccagca agccaatcca gtcagcgtcc agtttcagcg ccggagtcag
    37921 ttgctccagc gccacgccaa tcattaccag tacataacca tccagaaacg gaccaccact
    37981 tccccacagc aaaatgcggc ggtgaataga ggagaatttg agatcgtcaa agtttctgga
    38041 cggttgcatg ggttattcct gttttttgca atgagagtca gtgcggcgtt acgtatcaaa
    38101 ccaacatcag ccgtaacgga actccacacc aaaggtgccg cgcgggtatt cccactgttc
    38161 cagcgccgac cccagcccca gaatgcgaca ggtgccgcac tccagacatc cggcgtaatc
    38221 gaagcgcaca ctgccgtcat cctgcttctt gtacagacct gcggggcacg ctttcaccag
    38281 cagctccagc acctgtttat cagcatcggc cttcacaaca atgtgcggat gctcttcatc
    38341 gacattgaat ttattgacgc ccagtttgac gtccacattg acgggagaag tcataaaacg
    38401 gtcactcctt tcatgccatc cttgattaga ttgatgaagc ccactttctt accgtggcgg
    38461 aggattttct tgcgcatcag ttctggcgca ctgccatcaa tggtgaacag gtcacgcgcc
    38521 acgcccaccg ccagctccgg gtagccgctg aacatgcgtg ggttatcaag gaacgccggt
    38581 agtttctggt acatgcgcat atcgcgcagc gggccactct caagatgctg acgatattcc
    38641 gccagttttt gcttactgaa atcgtcgctt ttcatcgctg aaagcacggt ttttgctgca
    38701 gcttccccgg cggcaatcgc cagatccata ccgcgaatgg taaaaccgag gttcatacac
    38761 attccggcgg catcgccggc aatcaatacg ccgtcaccga ccagctccgg cagcatgttg
    38821 atgcctgctt ccggcactac gtgagcggaa tattccacca gcttgccacc cgcgatcagc
    38881 ggtgcaacgg ccggatgttg tttgaaatct tccagcattt gcggcaccga tttttttgcg
    38941 tcatgcagat gatgcagacc acaaaccagc cccagcgaca gggtattttc attggtataa
    39001 aggaagccgc cgcccatcag gccatcggtg ggtgatcccg caaacagaca agccgcgccc
    39061 tgattaccct gcaactgaaa acggtcttcg attaccgact tcggtaactc gatcagttcc
    39121 ttcacgccaa ccgccacatc cgtcggttta acgcgttttg ccatccccaa cttttcggca
    39181 aggatggaat tcaccccgtc agcaaggatc accgttttcg cttcaatcac atcgccatcg
    39241 gcttctacac cgacgacttt gccatcgcgc tgtacgaggt tatcgacacg aatcccggta
    39301 attaactgcg cgcccgcttc ttcggcctgc tccatcaacc aggcatcaaa tttactgcgc
    39361 aaaacggagt aagaacgctg ggatggcgag gtttcgtcac cgttgcagta gtccatagtc
    39421 atcgctgact tttccgtcat aaacgcgagt ttttcatggg tgatcaggcg ttctacgggg
    39481 gcggagtcgg cgaaaccagg aataatgtgt tccagactgt gggcatagag acgcccgccg
    39541 gtgacgttct tggcacctgc ggaattgcca cgctcgataa ctaacacttg cgcaccttcg
    39601 cgggcgagca ccagtgcggc aaccgaaccg gcaagccctg caccgacgat gatggcgtca
    39661 aagatatctt cggacataag agctccctgt cagcggcagg gccaaaatgc agccctgcca
    39721 gagtggatca acgcgctaaa gctgcggtca gcgccggaag gatcttcacg gcgtcgccaa
    39781 caatgccgta atccgcgtac tggaagatcg gcgcattttt atctttgttg atggcgaaaa
    39841 tggtttgcga tgcattagca ccaaccatgt gctggatctg cccggagatc cccaccgcca
    39901 ggtacagttc aggtttcagc atcaggttgg agataccgac atagcgttcg tgctccatcc
    39961 atttttcgtt ttccgccacc ggacgagaac aggccaactc cgcacctatc gccttgcaaa
    40021 gctgttctgc cagcgcaatg ttctctttgc tgccaatacc gcgaccgacg ctgaccacca
    40081 gacgggcttt gtcgagatcg acgctgttgc tctggcgcgc ctgggttgcc gtgcgggtaa
    40141 tcgccacagc cggagcctgc cactccacgg tgtgcgtttc gccagtgcgt gacgcgtctg
    40201 gctgagccgc atcgaacgtg ccgctgctga tggtcagtac cgcatacggc gtggcaatgc
    40261 gttcttcgcc aatcgccaga ccaccgtaaa ccatgtgttt cactgtcgct ttaccgtcct
    40321 gtacgctgac ggtgctggta tcgttagaca ccgccgcttt aaggcgataa cccagttttg
    40381 ccgccagtaa tttgccgcga cgggtgtttg gcagcagcac caggccgtct gcgccgtgct
    40441 ggcgaatagt gtcagccatg acaccggcgt aatcttcgat catccgatcg tccggtttgc
    40501 cgtttaattt ccagacatga ttagcgccga gctggattgc ctgtacgccg tcggcatcat
    40561 tgaggacaaa ggtgttgatt tgattagcta aagcctgcgc accgttcatc agttccggca
    40621 gacgagaagg ggtatcgctg aatacccaga cttgagaaaa cgtgttcata gcatcccctg
    40681 taattaaatg actttgcgaa gattttcagc aaatgcggcg atctgttctt cgccgtcgcc
    40741 ttcaatcacg atgcgctgac gttcgcgctg tttcggcgca gcaacctgtt gttcagacca
    40801 ggcgtccacg gcgttaaaac cgatatccgc cgccgaccat acctggactg gctttttcgc
    40861 cgcgccgaga atggctttca tcgaaggaat ttgtggggag ttgatatcag tggaaacagc
    40921 aacaaccgca ggcagcggaa tgcttaaggt ttcggtttca tcttccagtt cgcgctcaac
    40981 ggtgagggta tctgccgtca gggagataat tttgctgacg ccgttaactg ccggaatatt
    41041 gaggatttcg cccaccagca gaccaacctg ctgggcataa aggtcggaag aaccatcgcc
    41101 acagaggatc aaatcaaagc ctgctttctg ggcggctgca gccagtgcgc tcgccgtttg
    41161 ttgcggcagt gcctgctcga actggtcatc aatcaccaca atcagttcat ccgagccgcg
    41221 cgatagcaca tctttacgcc ctttggcgtt ggtcagggct ttaccgccca cacttaaggc
    41281 cgtcacctgc gcctctgctg cctgttgctt tagctggcaa gccgcttcaa tagcgttcag
    41341 atcgtattgg cttattttgg catcggcttt gctgaagtct aatgaaccat cagcattatt
    41401 gaccgcaata tcctgttcat caggcacgca cttatagcaa gtaataatct tcattgcatc
    41461 tccagaaatc atgaaggaaa cgtcgttcat atcggtggtg ttaattacag atatcacgcc
    41521 ctttaatttg gaaataaaac tttaatcacc aatattgaaa atgtcacacg cagataaatc
    41581 caactttcaa tattgttaag ttcctcacca atattgaaaa catggcgtag aaaaaagaaa
    41641 ttttcaatat tgctttatgg atcaccaata ttgaaagtca gatgctattt ttttactcac
    41701 aacacaacat aacaaactga ttaataatca aaattaacga gaaaatcgcc ctctattcac
    41761 ccgaaacaaa aatgtgataa caatcacaga atgcagctta ttgaataccc attatgagtt
    41821 agccattaac gcgtccacga ggttaataat aattatatta aatgttaaca aaaataaaac
    41881 aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa
    41941 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac
    42001 tttgttggct tacagtcaga gatctggatg cagcgaatgt cgttattaat gctgtattca
    42061 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
    42121 gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccacctg
    42181 aatttagtac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
    42241 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
    42301 cgaactcgac aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
    42361 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
    42421 aaatggaagt gattcgcccc agctcgacac tggtgccgct ggtaggtgaa aaacacgcca
    42481 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
    42541 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
    42601 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
    42661 tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
    42721 tgagcttcct gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc atcatgaact
    42781 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg
    42841 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
    42901 tgatttatgc tatccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc
    42961 gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc aacctggatc ctgtggactg
    43021 tactcggtag taacactctg ctgttgatag ataaaaacat catcaacatt ccaaatctga
    43081 tcgaacagta cggtgtggcg cgcgccatta tcgaaacctg ggccgctctg ccactcagca
    43141 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
    43201 cctgctctta taccctggcg atgtccactt gccgcgaagt acgcgatggt gaagaaccac
    43261 cgctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttttgctgg
    43321 cgctcggcgg cctgaaaccg attcaaactg ccattatcgc cggaggatgc ccgctgttct
    43381 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
    43441 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
    43501 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
    43561 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
    43621 gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc
    43681 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg ctgtaccagt
    43741 tgccgggcgg gttcaacacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
    43801 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
    43861 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
    43921 atggtagtaa gtgttttatt accagcagcg cctacacccc gtacatcgtg gtgatggcgc
    43981 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
    44041 cgggcatcaa agtgaccaaa cttgaaaagc tcggtctgcg tatggatagc tgctgtgaaa
    44101 tcacctttga cgacgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct
    44161 ttaaccgcgt caaagaagag ttcgatcatg aacgtttcct ggtagctctc accaactacg
    44221 gtacggcgat gtgcgccttt gaagatgcgg cgcgctacgc caaccagcgc gtgcagtttg
    44281 gcgaggctat tggccgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
    44341 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
    44401 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg
    44461 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
    44521 tctggcgtga cctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg cagatcctga
    44581 cgctgggtcg cgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc
    44641 taatgcgtct tatcaggcct acgggagcac gaatgtaggc cggataaagc gtttacgccg
    44701 catccggcag tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat
    44761 gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg
    44821 gccgttcgcc gggcaaatgt tcgcagaatg gggtgcggaa gttatctgga tcgagaacgt
    44881 cgcctgggcc gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt
    44941 gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat
    45001 ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat
    45061 taccgatgaa gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt
    45121 tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacta tcgcccaggc
    45181 ctttagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta
    45241 taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa
    45301 agtgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg
    45361 tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag
    45421 caaaggtaaa gatccctact acgccggttg cggtctgtat aaatgcgccg acggctacat
    45481 cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc
    45541 acatctactc ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg
    45601 cccttacggc ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc
    45661 agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc
    45721 ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat
    45781 ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca
    45841 aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg
    45901 ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta
    45961 aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa
    46021 tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc
    46081 ttgcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta
    46141 accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata
    46201 cgctagggat tcgcaaaggc gacaaggttg ctctacatct cgacaactgc ccggaattta
    46261 tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc
    46321 tgttgcgcga ggaaagcgcg tggatcctgc aaaatagcca ggcgtgcctg ctggtgacca
    46381 gtgcgcaatt ctatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc
    46441 acatttgcct gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg tttactcaac
    46501 tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg
    46561 cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt
    46621 acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg
    46681 tctacctgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg
    46741 cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg
    46801 gacaggtgca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt
    46861 tgatggtgca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt
    46921 atctcaactt gtcggagcag gaaaaagatg cgttttgtga acgcttcggc gttcgcttgc
    46981 tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctggcgata
    47041 aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg
    47101 atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag
    47161 ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag
    47221 ccgacggctg gctacatacc ggcgataccg gatactgcga cgaagagggc tttttttatt
    47281 tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc
    47341 tggaaaatat catcgccacc catccaaaaa ttcaggatat cgtggttgtg ggtattaaag
    47401 attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga
    47461 gcgaagagga atttttccgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt
    47521 atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc
    47581 tgaaataaat gaaacagcag ggaactaccc tgccagctaa taatcacgct ataaaacaat
    47641 atgcattttt tgcagggatg ttgtcgtccc tgaaaaagca aaaatggaga aaaggaatga
    47701 gtgaatcatt acatctgacc cgcaatggat caattctgga aattaccctt gatcgtccaa
    47761 aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc
    47821 gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaag ttcttttccg
    47881 cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg
    47941 gtggatttgc gggattaacc gaaattttca atctcgacaa accggttatc gcagctgtga
    48001 acggctatgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg
    48061 ccgataacgc cagcttcgcc ctgccggaag ccaagctggg catcgttcct gacagcggcg
    48121 gcgtgctgcg tctaccgaag atcctgccgc ctgccatcgt caatgaaatg gtgatgaccg
    48181 gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc
    48241 aggcggaact gatggataac gcccgcgaac tggcgcagca gctggttaac agcgccccgc
    48301 tggcgattgc ggcgctgaaa gagatcttcc gcaccaccag cgaaatgccg gtagaagaag
    48361 cgtatcgcta tattcgcagc ggcgtgttga aacactatcc gtcggttctg cattcggagg
    48421 atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt
    48481 aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt
    48541 tgtccatccc agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg
    48601 cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat
    48661 tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg
    48721 ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg
    48781 gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg ttgccgccat
    48841 gagctttgtc aaagcaggct ttcacggcga gaaacgccag ttgttgatgg gtacgccagc
    48901 ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga
    48961 gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacacagc cactgaggca
    49021 aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc
    49081 ttgccggatg cgacctggtt gctggataag atgctacaac atcgcatctg gcaagcagga
    49141 cgcccggtat caacgagata aaattaacga cgcatactct ttgacaacat catctgccac
    49201 ttctgctcgc ggttaagttc cgtgagcgga acggcaggaa tacgcggcgt ctttcttacg
    49261 ccgactaatt tatcccggct gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg
    49321 accactttta ccagtcgctg gcactggcat ccccgtcctt ccagcttatt agggatcatc
    49381 ttaacttcgc agcttatttc tgcgacttcc gacagaatat aagtcagggt attaaccgcc
    49441 ttgctgtgtt caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca
    49501 gccatcatcc attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata
    49561 cgaactccag tttcacaaat taaaagtctg gctatgactc gttttgaagc aattaaacaa
    49621 ggccatatta aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag
    49681 ctaaatcctg cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa
    49741 aaaaaacggt cctcatcaga gaaccgtatt acttattcga tcaaattagc ggatgaaaaa
    49801 tatccgccat gacacgctat tatttgatct gcgcgtgcat ttcctgcacc gaaattactt
    49861 tttcggtcgc gtcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt
    49921 cataatgcac tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcgc
    49981 gacggcctga agtggtgttg atgatgtagg tatattcgcc attcttgatg cggtcctgaa
    50041 tgtgcggacg gccttcatgc accttgttca ccagacgcgg attaatgccc gcttcgccca
    50101 gcacaatcgc cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg
    50161 ccaggtccac cacgcgttct ttatcgcctt cgcgcacgga aagcaacgca cgaccgtgtt
    50221 tcttcatggt ggagttgctg cccagctgcg ctttggcaaa cgcttcagcg aaggtgcggc
    50281 ccacgcccat gacttccccg gtagagcgca tttctggccc taacagcggg tcaacacccg
    50341 ggaatttatt gaacggcagc accacttctt tcaccgagta gtacggcggg ataacttctt
    50401 tggttacgcc ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca
    50461 gcggaacgcc ggtggctttg gagacgaacg gaacggtacg cgccgcacgc gggttaactt
    50521 caatcaggta gacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct
    50581 gcaattcgaa ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta
    50641 aggtgtaggc tggcagagaa catgcggagt caccggagtg cacgcccgcc tgctcgatgt
    50701 gctccataat gccgccaatc agcaccattt caccgtcgca gatggcatcc acgtcaactt
    50761 ccaccgcgtc atcgaggaag tgatccagca acactggcgc atcgttagac acgctgaccg
    50821 ccgtctggaa gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc
    50881 cgagaacgta agacggacgt accaccagcg ggtagccaat ctctttcgcc ttctctaccg
    50941 ccatttcaat agcggtaacg gtggcgttcg ccggttgttt cagtttcaga cgctcaaccg
    51001 catgctggaa gcgttcacgg tcttctgcac ggtcgatagc atccgggctg gtgccgataa
    51061 ccggtacgcc agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact
    51121 gaacgataac gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta
    51181 ccggctcgaa gtagaggcga tcggaggtgt cgtagtcggt agagacggtt tccgggttac
    51241 agttaaccat aatggtctca aaaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc
    51301 agtagtcgaa ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt
    51361 tttcacggtc ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag
    51421 cggtgtcggt ggcaaactct gccgcacagg tatccacacg cttgtagacc gggtgcaggt
    51481 catattggtc acgcagctta cggatttccg cttcgcgtac acccgcgagt tttgccagac
    51541 gcgcatcggc aaagcctttg cgtttcagct ggcgcaggaa gtcagcgttc aggccagtga
    51601 tgcccacttc cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc
    51661 agcggtcaat gttggtcagg ttgaagacgc cgtccacaga caggcccgca cggaaagcat
    51721 cggcgatgta ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta
    51781 acgcttccgg gtcatcgagg ctcactttcg ggtcgaatcc agtcgcaccg acttccaggc
    51841 cgcgcagcgc tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa
    51901 ccgatttcat ctgagtggtc agacggtcgt tagcaccagc gaatttttcg aagttgaagc
    51961 gaggaatttt agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc
    52021 cagtgatgtc gttcatcagt tcgtcgaggg tgtaacccac cgccagtttc gccgccactt
    52081 tagcaatcgg gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt
    52141 tcatttcgat aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac
    52201 caccggtttc aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat
    52261 attctttgtc ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtatggatgc
    52321 ccatcgcatc aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca
    52381 ccacttccat ctcgtactct ttccagccga tcagcgactc atcaatcagc agctctttgg
    52441 ttggggagag atccagaccg cgggcgcaaa tttcttcaaa ctcttcgcgg ttataagcga
    52501 taccgccgcc gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt
    52561 cagcggcaac cgccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca
    52621 gaccaatttt cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat
    52681 cggcggtcgc accaatcata gtcacgccga actcttccaa cacgccctga cgctccagct
    52741 ccagcgcgca gttcagcgcc gtctgaccgc ccatcgttgg cagcaccgcg tccgggcgct
    52801 ctttttcaat aatcttgcgt actacttccc agtgaatcgg ctcgatgtag gtcgcatcgg
    52861 ccatttccgg gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac
    52921 cctcttcgcg cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac
    52981 cgataacaat cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca
    53041 tggctctttt actcctgatt acttagcggt tttacggtac tgctcaatta actcgataaa
    53101 gtggtcgaac aacggcgctg cgtcgtgtgg cccagggctg gcttccgggt gaccctggaa
    53161 gctgaacgcc ggtttatcag tgcgatgaat gccctgtaac gtaccgtcga acagggattt
    53221 atgcgtgaca cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg
    53281 ggcggtgatc atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg
    53341 accaaatttc attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag
    53401 gcagatgccg aataccggaa tatcggtttc gaggaatttc tggatggcgg taatggcgta
    53461 atcgcacggg gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt
    53521 cagcacatct tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac
    53581 cagcatccgc aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag
    53641 ctcgtcttct tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca
    53701 gctataggct tctgcggtgg tcacttcttt tgccagatcc atgccattca gacctgggaa
    53761 cgcgcgggct ttttctaacg ccaacgccgc atccgggtta tcgcctgcga taatgcagcc
    53821 attctgtgcg cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat
    53881 cgccacgatg ttatggcgct tcaggtaaga agaaaggtct tcggtattac ggaagttgct
    53941 ggcaatcagc ggcagatcgc gaatcaccag accttgtgca tgtacctgag aagattcttc
    54001 atcggcgtca ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg
    54061 agaataggaa ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac
    54121 ttccccaacc gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc
    54181 cagaaccaat agcgctgact taatcaaaac accctccaga gaatattcac tcactttatt
    54241 tgcatattaa ttcacaatga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct
    54301 ggcaaacggc ggcattctgg agatatgggc gctaaaagtc aacttaatgg cgggattttt
    54361 tatattattt tgtggctcat gcatgtttta caggcattaa gcgacaaaaa gatcatataa
    54421 cctgttctgg aaaacgcttg cgcagcaaga agattgcatg aaataaaagc gagcagggaa
    54481 aagtggacca aatggtcaaa atttacatta aaacaacaaa aaccgaagac aaaacatgta
    54541 atttttggca cttatagaca atttaaaaac ataacactaa aaat
//
LOCUS       DAZFVO020000010        54138 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFVO020000010 DAZFVO020000000
VERSION     DAZFVO020000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 54138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.010"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            354..1082
                     /gene="mpaA"
                     /locus_tag="ACHKNB_RS02640"
                     /old_locus_tag="pgaptmp_000528"
     CDS             354..1082
                     /gene="mpaA"
                     /locus_tag="ACHKNB_RS02640"
                     /old_locus_tag="pgaptmp_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HGU4194107.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1057..2022)
                     /gene="ycjG"
                     /locus_tag="ACHKNB_RS02645"
                     /old_locus_tag="pgaptmp_000529"
     CDS             complement(1057..2022)
                     /gene="ycjG"
                     /locus_tag="ACHKNB_RS02645"
                     /old_locus_tag="pgaptmp_000529"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HGU4194108.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMVNSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2141..2647
                     /gene="tpx"
                     /locus_tag="ACHKNB_RS02650"
                     /old_locus_tag="pgaptmp_000530"
     CDS             2141..2647
                     /gene="tpx"
                     /locus_tag="ACHKNB_RS02650"
                     /old_locus_tag="pgaptmp_000530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGU4194109.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2691..4232)
                     /gene="tyrR"
                     /locus_tag="ACHKNB_RS02655"
                     /old_locus_tag="pgaptmp_000531"
     CDS             complement(2691..4232)
                     /gene="tyrR"
                     /locus_tag="ACHKNB_RS02655"
                     /old_locus_tag="pgaptmp_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HGU4194110.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4380..5441)
                     /gene="ycjF"
                     /locus_tag="ACHKNB_RS02660"
                     /old_locus_tag="pgaptmp_000532"
     CDS             complement(4380..5441)
                     /gene="ycjF"
                     /locus_tag="ACHKNB_RS02660"
                     /old_locus_tag="pgaptmp_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HGU4194111.1"
                     /translation="MTEPLKLRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5438..6835)
                     /gene="ycjX"
                     /locus_tag="ACHKNB_RS02665"
                     /old_locus_tag="pgaptmp_000533"
     CDS             complement(5438..6835)
                     /gene="ycjX"
                     /locus_tag="ACHKNB_RS02665"
                     /old_locus_tag="pgaptmp_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HGU4194112.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTNDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            6991..7989
                     /gene="ycjW"
                     /locus_tag="ACHKNB_RS02670"
                     /old_locus_tag="pgaptmp_000534"
     CDS             6991..7989
                     /gene="ycjW"
                     /locus_tag="ACHKNB_RS02670"
                     /old_locus_tag="pgaptmp_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4194113.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8100..9004)
                     /gene="ompG"
                     /locus_tag="ACHKNB_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /pseudo
     CDS             complement(8100..9004)
                     /gene="ompG"
                     /locus_tag="ACHKNB_RS02675"
                     /old_locus_tag="pgaptmp_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9049..10131)
                     /locus_tag="ACHKNB_RS02680"
                     /old_locus_tag="pgaptmp_000536"
     CDS             complement(9049..10131)
                     /locus_tag="ACHKNB_RS02680"
                     /old_locus_tag="pgaptmp_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4194114.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(10145..10804)
                     /gene="pgmB"
                     /locus_tag="ACHKNB_RS02685"
                     /old_locus_tag="pgaptmp_000537"
     CDS             complement(10145..10804)
                     /gene="pgmB"
                     /locus_tag="ACHKNB_RS02685"
                     /old_locus_tag="pgaptmp_000537"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HGU4194115.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(10801..12811)
                     /locus_tag="ACHKNB_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /pseudo
     CDS             complement(10801..12811)
                     /locus_tag="ACHKNB_RS02690"
                     /old_locus_tag="pgaptmp_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
     gene            complement(12808..13863)
                     /gene="ycjS"
                     /locus_tag="ACHKNB_RS02695"
                     /old_locus_tag="pgaptmp_000539"
     CDS             complement(12808..13863)
                     /gene="ycjS"
                     /locus_tag="ACHKNB_RS02695"
                     /old_locus_tag="pgaptmp_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4194116.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(13873..14661)
                     /gene="ycjR"
                     /locus_tag="ACHKNB_RS02700"
                     /old_locus_tag="pgaptmp_000540"
     CDS             complement(13873..14661)
                     /gene="ycjR"
                     /locus_tag="ACHKNB_RS02700"
                     /old_locus_tag="pgaptmp_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HGU4194117.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRTLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(14679..15731)
                     /gene="ycjQ"
                     /locus_tag="ACHKNB_RS02705"
                     /old_locus_tag="pgaptmp_000541"
     CDS             complement(14679..15731)
                     /gene="ycjQ"
                     /locus_tag="ACHKNB_RS02705"
                     /old_locus_tag="pgaptmp_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HGU4194118.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(15762..16604)
                     /gene="ycjP"
                     /locus_tag="ACHKNB_RS02710"
                     /old_locus_tag="pgaptmp_000542"
     CDS             complement(15762..16604)
                     /gene="ycjP"
                     /locus_tag="ACHKNB_RS02710"
                     /old_locus_tag="pgaptmp_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4194119.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16591..17472)
                     /gene="ycjO"
                     /locus_tag="ACHKNB_RS02715"
                     /old_locus_tag="pgaptmp_000543"
     CDS             complement(16591..17472)
                     /gene="ycjO"
                     /locus_tag="ACHKNB_RS02715"
                     /old_locus_tag="pgaptmp_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGU4194120.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17493..18785)
                     /gene="ycjN"
                     /locus_tag="ACHKNB_RS02720"
                     /old_locus_tag="pgaptmp_000544"
     CDS             complement(17493..18785)
                     /gene="ycjN"
                     /locus_tag="ACHKNB_RS02720"
                     /old_locus_tag="pgaptmp_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4194121.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTVPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(18799..20478)
                     /gene="ycjM"
                     /locus_tag="ACHKNB_RS02725"
                     /old_locus_tag="pgaptmp_000545"
     CDS             complement(18799..20478)
                     /gene="ycjM"
                     /locus_tag="ACHKNB_RS02725"
                     /old_locus_tag="pgaptmp_000545"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HGU4194122.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(20691..21005)
                     /gene="pspE"
                     /locus_tag="ACHKNB_RS02730"
                     /old_locus_tag="pgaptmp_000546"
     CDS             complement(20691..21005)
                     /gene="pspE"
                     /locus_tag="ACHKNB_RS02730"
                     /old_locus_tag="pgaptmp_000546"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HGU4194123.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(21080..21301)
                     /gene="pspD"
                     /locus_tag="ACHKNB_RS02735"
                     /old_locus_tag="pgaptmp_000547"
     CDS             complement(21080..21301)
                     /gene="pspD"
                     /locus_tag="ACHKNB_RS02735"
                     /old_locus_tag="pgaptmp_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HGU4194124.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(21310..21669)
                     /gene="pspC"
                     /locus_tag="ACHKNB_RS02740"
                     /old_locus_tag="pgaptmp_000548"
     CDS             complement(21310..21669)
                     /gene="pspC"
                     /locus_tag="ACHKNB_RS02740"
                     /old_locus_tag="pgaptmp_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HGU4194125.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(21669..21893)
                     /gene="pspB"
                     /locus_tag="ACHKNB_RS02745"
                     /old_locus_tag="pgaptmp_000549"
     CDS             complement(21669..21893)
                     /gene="pspB"
                     /locus_tag="ACHKNB_RS02745"
                     /old_locus_tag="pgaptmp_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HGU4194126.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(21947..22615)
                     /gene="pspA"
                     /locus_tag="ACHKNB_RS02750"
                     /old_locus_tag="pgaptmp_000550"
     CDS             complement(21947..22615)
                     /gene="pspA"
                     /locus_tag="ACHKNB_RS02750"
                     /old_locus_tag="pgaptmp_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HGU4194127.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            22782..23759
                     /gene="pspF"
                     /locus_tag="ACHKNB_RS02755"
                     /old_locus_tag="pgaptmp_000551"
     CDS             22782..23759
                     /gene="pspF"
                     /locus_tag="ACHKNB_RS02755"
                     /old_locus_tag="pgaptmp_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HGU4194128.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(23879..25144)
                     /gene="puuE"
                     /locus_tag="ACHKNB_RS02760"
                     /old_locus_tag="pgaptmp_000552"
     CDS             complement(23879..25144)
                     /gene="puuE"
                     /locus_tag="ACHKNB_RS02760"
                     /old_locus_tag="pgaptmp_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HGU4194129.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(25182..26462)
                     /gene="puuB"
                     /locus_tag="ACHKNB_RS02765"
                     /old_locus_tag="pgaptmp_000553"
     CDS             complement(25182..26462)
                     /gene="puuB"
                     /locus_tag="ACHKNB_RS02765"
                     /old_locus_tag="pgaptmp_000553"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HGU4194130.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(26464..27951)
                     /gene="puuC"
                     /locus_tag="ACHKNB_RS02770"
                     /old_locus_tag="pgaptmp_000554"
     CDS             complement(26464..27951)
                     /gene="puuC"
                     /locus_tag="ACHKNB_RS02770"
                     /old_locus_tag="pgaptmp_000554"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HGU4194131.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(28226..28783)
                     /gene="puuR"
                     /locus_tag="ACHKNB_RS02775"
                     /old_locus_tag="pgaptmp_000555"
     CDS             complement(28226..28783)
                     /gene="puuR"
                     /locus_tag="ACHKNB_RS02775"
                     /old_locus_tag="pgaptmp_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HGU4194132.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(28810..29574)
                     /gene="puuD"
                     /locus_tag="ACHKNB_RS02780"
                     /old_locus_tag="pgaptmp_000556"
     CDS             complement(28810..29574)
                     /gene="puuD"
                     /locus_tag="ACHKNB_RS02780"
                     /old_locus_tag="pgaptmp_000556"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HGU4194133.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            29786..31204
                     /gene="puuA"
                     /locus_tag="ACHKNB_RS02785"
                     /old_locus_tag="pgaptmp_000557"
     CDS             29786..31204
                     /gene="puuA"
                     /locus_tag="ACHKNB_RS02785"
                     /old_locus_tag="pgaptmp_000557"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HGU4194134.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            31353..31517
                     /locus_tag="ACHKNB_RS02790"
                     /old_locus_tag="pgaptmp_000558"
     CDS             31353..31517
                     /locus_tag="ACHKNB_RS02790"
                     /old_locus_tag="pgaptmp_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194135.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            31507..32882
                     /gene="puuP"
                     /locus_tag="ACHKNB_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /pseudo
     CDS             31507..32882
                     /gene="puuP"
                     /locus_tag="ACHKNB_RS02795"
                     /old_locus_tag="pgaptmp_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
     gene            33016..33261
                     /gene="ymjA"
                     /locus_tag="ACHKNB_RS02800"
                     /old_locus_tag="pgaptmp_000560"
     CDS             33016..33261
                     /gene="ymjA"
                     /locus_tag="ACHKNB_RS02800"
                     /old_locus_tag="pgaptmp_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HGU4194136.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            33574..35217
                     /gene="sapA"
                     /locus_tag="ACHKNB_RS02805"
                     /old_locus_tag="pgaptmp_000561"
     CDS             33574..35217
                     /gene="sapA"
                     /locus_tag="ACHKNB_RS02805"
                     /old_locus_tag="pgaptmp_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HGU4194137.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            35214..36179
                     /gene="sapB"
                     /locus_tag="ACHKNB_RS02810"
                     /old_locus_tag="pgaptmp_000562"
     CDS             35214..36179
                     /gene="sapB"
                     /locus_tag="ACHKNB_RS02810"
                     /old_locus_tag="pgaptmp_000562"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HGU4194138.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            36166..37056
                     /gene="sapC"
                     /locus_tag="ACHKNB_RS02815"
                     /old_locus_tag="pgaptmp_000563"
     CDS             36166..37056
                     /gene="sapC"
                     /locus_tag="ACHKNB_RS02815"
                     /old_locus_tag="pgaptmp_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HGU4194139.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLSGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            37056..38048
                     /gene="sapD"
                     /locus_tag="ACHKNB_RS02820"
                     /old_locus_tag="pgaptmp_000564"
     CDS             37056..38048
                     /gene="sapD"
                     /locus_tag="ACHKNB_RS02820"
                     /old_locus_tag="pgaptmp_000564"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HGU4194140.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            38050..38856
                     /gene="sapF"
                     /locus_tag="ACHKNB_RS02825"
                     /old_locus_tag="pgaptmp_000565"
     CDS             38050..38856
                     /gene="sapF"
                     /locus_tag="ACHKNB_RS02825"
                     /old_locus_tag="pgaptmp_000565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HGU4194141.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            38924..39277
                     /gene="ycjD"
                     /locus_tag="ACHKNB_RS02830"
                     /old_locus_tag="pgaptmp_000566"
     CDS             38924..39277
                     /gene="ycjD"
                     /locus_tag="ACHKNB_RS02830"
                     /old_locus_tag="pgaptmp_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HGU4194142.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            39645..40433
                     /gene="fabI"
                     /locus_tag="ACHKNB_RS02835"
                     /old_locus_tag="pgaptmp_000567"
     CDS             39645..40433
                     /gene="fabI"
                     /locus_tag="ACHKNB_RS02835"
                     /old_locus_tag="pgaptmp_000567"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HGU4194143.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            40578..41705
                     /gene="yciW"
                     /locus_tag="ACHKNB_RS02840"
                     /old_locus_tag="pgaptmp_000568"
     CDS             40578..41705
                     /gene="yciW"
                     /locus_tag="ACHKNB_RS02840"
                     /old_locus_tag="pgaptmp_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HGU4194144.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            41773..43707
                     /gene="rnb"
                     /locus_tag="ACHKNB_RS02845"
                     /old_locus_tag="pgaptmp_000569"
     CDS             41773..43707
                     /gene="rnb"
                     /locus_tag="ACHKNB_RS02845"
                     /old_locus_tag="pgaptmp_000569"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HGU4194145.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(43785..43889)
                     /locus_tag="ACHKNB_RS02850"
                     /old_locus_tag="pgaptmp_000570"
     CDS             complement(43785..43889)
                     /locus_tag="ACHKNB_RS02850"
                     /old_locus_tag="pgaptmp_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194146.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            43943..45928
                     /gene="pdeR"
                     /locus_tag="ACHKNB_RS02855"
                     /old_locus_tag="pgaptmp_000571"
     CDS             43943..45928
                     /gene="pdeR"
                     /locus_tag="ACHKNB_RS02855"
                     /old_locus_tag="pgaptmp_000571"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4194147.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(45910..46038)
                     /gene="ymiD"
                     /locus_tag="ACHKNB_RS02860"
                     /old_locus_tag="pgaptmp_000572"
     CDS             complement(45910..46038)
                     /gene="ymiD"
                     /locus_tag="ACHKNB_RS02860"
                     /old_locus_tag="pgaptmp_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HGU4194148.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            46075..46248
                     /gene="yciZ"
                     /locus_tag="ACHKNB_RS02865"
                     /old_locus_tag="pgaptmp_000573"
     CDS             46075..46248
                     /gene="yciZ"
                     /locus_tag="ACHKNB_RS02865"
                     /old_locus_tag="pgaptmp_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HGU4194149.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            46338..47087
                     /gene="yciT"
                     /locus_tag="ACHKNB_RS02870"
                     /old_locus_tag="pgaptmp_000574"
     CDS             46338..47087
                     /gene="yciT"
                     /locus_tag="ACHKNB_RS02870"
                     /old_locus_tag="pgaptmp_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HGU4194150.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            47356..47574
                     /gene="osmB"
                     /locus_tag="ACHKNB_RS02875"
                     /old_locus_tag="pgaptmp_000575"
     CDS             47356..47574
                     /gene="osmB"
                     /locus_tag="ACHKNB_RS02875"
                     /old_locus_tag="pgaptmp_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HGU4194151.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(47700..48026)
                     /gene="yciH"
                     /locus_tag="ACHKNB_RS02880"
                     /old_locus_tag="pgaptmp_000576"
     CDS             complement(47700..48026)
                     /gene="yciH"
                     /locus_tag="ACHKNB_RS02880"
                     /old_locus_tag="pgaptmp_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HGU4194152.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(48026..48763)
                     /gene="pyrF"
                     /locus_tag="ACHKNB_RS02885"
                     /old_locus_tag="pgaptmp_000577"
     CDS             complement(48026..48763)
                     /gene="pyrF"
                     /locus_tag="ACHKNB_RS02885"
                     /old_locus_tag="pgaptmp_000577"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGU4194153.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(48956..50125)
                     /gene="lapB"
                     /locus_tag="ACHKNB_RS02890"
                     /old_locus_tag="pgaptmp_000578"
     CDS             complement(48956..50125)
                     /gene="lapB"
                     /locus_tag="ACHKNB_RS02890"
                     /old_locus_tag="pgaptmp_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HGU4194154.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(50132..50440)
                     /gene="lapA"
                     /locus_tag="ACHKNB_RS02895"
                     /old_locus_tag="pgaptmp_000579"
     CDS             complement(50132..50440)
                     /gene="lapA"
                     /locus_tag="ACHKNB_RS02895"
                     /old_locus_tag="pgaptmp_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HGU4194155.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(50589..51353)
                     /gene="pgpB"
                     /locus_tag="ACHKNB_RS02900"
                     /old_locus_tag="pgaptmp_000580"
     CDS             complement(50589..51353)
                     /gene="pgpB"
                     /locus_tag="ACHKNB_RS02900"
                     /old_locus_tag="pgaptmp_000580"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HGU4194156.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            51523..52113
                     /gene="ribA"
                     /locus_tag="ACHKNB_RS02905"
                     /old_locus_tag="pgaptmp_000581"
     CDS             51523..52113
                     /gene="ribA"
                     /locus_tag="ACHKNB_RS02905"
                     /old_locus_tag="pgaptmp_000581"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HGU4194157.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(52177..>54138)
                     /gene="acnA"
                     /locus_tag="ACHKNB_RS02910"
                     /old_locus_tag="pgaptmp_000582"
     CDS             complement(52177..>54138)
                     /gene="acnA"
                     /locus_tag="ACHKNB_RS02910"
                     /old_locus_tag="pgaptmp_000582"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HGU4194158.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        12867 a        14431 c        13496 g        13344 t
ORIGIN      
        1 atcaatcaac ccgtgactga tggtgacaag ccattattca tgtaaatcaa ttacatagtt
       61 accatcgtaa tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa
      121 tccgatgcga ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt
      181 acgacttaca tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa
      241 cttccggcaa aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa
      301 tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg
      361 taaaccgccc acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt
      421 cattattggg cgcgccgtta atctggtttc ctgcccctgc agccagtcgc gaaagtggtt
      481 tgattctggc tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc
      541 tgcggacttt gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg
      601 gctgccagct ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg
      661 cggcgaactg gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg
      721 atgtcgtatt actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc
      781 agcttataca ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta
      841 ttgaagatcc cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc
      901 tggtaaccag cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca
      961 acttgcattg catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat
     1021 atctctttgc gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt
     1081 gaactgaagc gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa
     1141 actgacctgc ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca
     1201 gcccagcatc agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt
     1261 cagaccaccg gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt
     1321 cagattgcta cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt
     1381 ctccagcgcc gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc
     1441 cgccaatagc tggcaacgcg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac
     1501 gatcagcgtc gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg
     1561 gttatccagc ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt
     1621 gaccatctga tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag
     1681 cgttatgccg atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa
     1741 tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg
     1801 tgtcagccct ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc
     1861 actttctcca taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc
     1921 cagttcaacc accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt
     1981 atgtaagggc caggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg
     2041 cggtggtctg cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac
     2101 taggtgtgac gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt
     2161 ccagggcaac ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac
     2221 ttttactctc gtagcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg
     2281 caaagtgctg aacattttcc cgagtattga taccggagtt tgcgccgcat cagtacgtaa
     2341 gtttaaccaa ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct
     2401 gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc
     2461 cactttccgt aacgctgaat tcctgcaagc ctacggtgtg gcaattgctg atggcccact
     2521 gaaaggtctg gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag
     2581 ccagctggtg gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa
     2641 agcataattt aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg
     2701 ttcttcttct gactcagacc atattcccgc aacttattgg caatcgcggt atgtgaaacg
     2761 ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc
     2821 gagcgttcaa aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg
     2881 gctaccgttg cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg
     2941 tccagttgtg tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac
     3001 gcataacgcg taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc
     3061 tcgtcggcaa agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca
     3121 cgtagcggcg gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac
     3181 atgccttttt gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg
     3241 acatgcacct catggtcttc gccaacccga cggaaagtgc catcattaag gaaacgcagt
     3301 aatttcgcct gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg
     3361 ttcgcctgct caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg
     3421 accgcatctt ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg
     3481 cttgcctgat ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc
     3541 agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc
     3601 gggctgacgg cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac
     3661 tggcggccca tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga
     3721 tcattttcat cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata
     3781 acgacatgct cgttatggga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag
     3841 ccgttaatca attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cgcaaaaagc
     3901 tgacagctcg ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt
     3961 tcgggcaacg cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac
     4021 gggacagtac gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg
     4081 aaactctcaa actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca
     4141 ataccgcgta aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg
     4201 agtcggtctt cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat
     4261 taatatgatg atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca
     4321 caaagacgga aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat
     4381 tatttttcgc tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga
     4441 cgacggaaat ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc
     4501 tccatagctt taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg
     4561 cgggtagaca aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc
     4621 agttcgctgg ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg
     4681 ctgtaatacc ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg
     4741 cgccaggcga taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt
     4801 gattccgccg ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc
     4861 aaatgcgcat acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac
     4921 cagcgttgca gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa
     4981 aatgcgcggc ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca
     5041 tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca
     5101 gcgccgataa tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc
     5161 caggcattca ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc
     5221 ccgcccatca ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc
     5281 gcttcaactt gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc
     5341 gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc
     5401 ggaccgtcga aatcaatacg tagttttaac ggttcggtca tcgcaattta tctcctatta
     5461 aaaattcaag cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct
     5521 gcgggcgaaa cgcttcaaac tggaaccctt gcttgtccca gaacgcctga ccgggcaaac
     5581 gcgcaggaac ttcaccggga taaacggtga gtggcgcacc atcgctgagc cggttaccgc
     5641 gcaatgccgg aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg
     5701 aggccagccc caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct
     5761 gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg atcgttggtc acatggtccg
     5821 ctttagtggc agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg
     5881 tacgctgccc gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa
     5941 atgcctgtgg cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa
     6001 agcgcagaaa atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc
     6061 gctcgcgcag cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc
     6121 aggcatcgac atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcccg
     6181 gcaagacaaa gcgcccaggc tgaataaagt gcagcccctg ctgtttacag tggtggagat
     6241 aatcggtcca cgcggcggca atgtctgcca gccggttttc gtcggcaggc gctagcgggt
     6301 ccagcccttc gcacatcatt cgccatttga ccgaccattc gccacgctga ccattgagta
     6361 agcccgtcat ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca
     6421 accattcgcc gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc
     6481 gtagcagcga atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg
     6541 ttggcgtcgg ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga
     6601 agcgcggaat gccaaagtca cgctggggaa tgcgtttcac gcccagcagg cgctcttcac
     6661 gcaccgcact taacagcggc aaacgtgctc cggcgtgaat attaagcaac tgattaacca
     6721 tcgcggtgat aaacgctgtt ttgccgctgc ggctaagtcc ggttacagcg aggcgcagat
     6781 gtctgtcgac accccgattc accagcgcat tgagttcatt tttaagtcgc ttcattgctg
     6841 tcctgtgcta ctctgaagac cagaaaaaaa atcattcgta aggatatgaa tgtgggggcc
     6901 aggccattga ttatcaactg cccgtgttgt catcgaccgg cgaactccat aagatgtgca
     6961 gcattcacgt tcggacggag ttcctgacct atgtccccta ctatttatga tattgccagg
     7021 gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga ataagcaaac taatatctcc
     7081 ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat tacagtatca gccaaacaag
     7141 ctggcccgcg cgctgacctc ttcgggtttt gatgccatta tggtgatttc tacccgttcg
     7201 accaaaacta cggcgggtaa tccgtttttc tctgaagttc tacatgccat caccgccaaa
     7261 gcggaagaag aaggtttcga cgtgatattg cagacgtcgc acaacctggc agaagactta
     7321 caaaaatgtg aaagcaaaat taagcagaaa atgattaaag gcattattat gctaagttcg
     7381 ccagcggatg agtcattttt tgcccaactt gataaatacg atattcctgt cgtggtgatt
     7441 ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg ataccgataa ttttggcgac
     7501 agcattgcgt tgaccgatgc attaattgaa agtgggcatc aaaatattgc ctgcctgcat
     7561 gctccgcttg atgtccatgt ttcagtggat cgggtaaatg gttataagca gagcctggct
     7621 gcgcataata ttgcagtgcg tgatgaatgg attgttgacg gcggttatac ccatgaaaca
     7681 gcactgcaag ccgcgcggga attattaagc cagtcaccgt tgcctgaggc agtgtttgct
     7741 actgacagcc tgaaattaat gagtatttat cgtgcggcag cagagaaaaa tattgctatt
     7801 ccgcagcagt tagcggtggt gggttatagt aatgaaacgc tgtcgtttat tttaacgcct
     7861 gcgccaggcg gtattgatat cccaacacag gagttaggtc aacaaagctg tgagttatta
     7921 ttccgcttaa tttctggaaa accgtcacca cagaatatta ccgttgccac gcatatgacg
     7981 ctgaaataaa taccagctac cttgcctgaa tgccggatgc gatgctagcg catcttatcc
     8041 ggcctacatt tatagttcgg ataaagtact cgtgccgcat ccggcaattt agcccattat
     8101 cagaacgagt aatttacgcc gacacctgca taatggaatt tatcgctgtc gccttcgtcg
     8161 tgatcctgcc actcaaacgc gtattccagc gaaacggaaa gcccgttctg gaaatcataa
     8221 ccgtaaaata aacctacacg gttaaaatca tggccttcac gttcaatatc atcctgccag
     8281 tcccagttac tccagcgatc cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg
     8341 agcgtcagcg gcaaataggc gcgaatttct tgcgtagaaa actcaccgtt attgcggctg
     8401 tcgtccatat taaagccgcg ctcgagataa tagttcactc gcaaggcaac cgtttcgttg
     8461 aaggtatatt gcaggcctgt ttccgtttcg actcgggtat cagcgtaacc ggtcgtgttc
     8521 agatcgttgg caaatttata catcgacaac caaccgttga aacgtaaatc gtcagtcagt
     8581 ttcacatccc agtctggcgc gattttccag cgctgcatat tcgccgtgtc tttacccggt
     8641 tcatcaacgt agtgataacc ataattacgg aaaccgccgg tcaggccgaa actgaaatca
     8701 tcgtttcgag gaactgataa tgcacctcca gctccgggcg atcaaaccac gttccacgtt
     8761 tacccgcgct ataatctacc ggcccttcct gataataggc cagagcaatt ctccacggcc
     8821 cgttggcggc attaaaatag actgaaggct ccgccagccc atccatatct tcgccataac
     8881 cctcgacgtt ttctatttcg tacatcgcgc cgatattaaa gtgccagtcg ttcctttcct
     8941 cggcctgtgc gcaggccatt cccgcacaca tcaccagtgc ggtacagggt aataactttt
     9001 tcattattgt tccttgtatt attctggggg aatgccttcc ccctgtattt agcgaattgc
     9061 tatttccgtt tctgcatcaa agaaatgaca tttcgtcata tcaaaatgaa tagtgatatt
     9121 ttctcctgca tgataatcat ttaacgcacc agcacggacc actaactcgt gccccccaac
     9181 cgtggtgtag agcataaatt ccgcaccggt taattctgcc acgctaattt tggcggaaat
     9241 gttattttcc tcttgcgcgt ccggatgaat atcttccgga cgtattccca tcacgatggg
     9301 cttatgcaaa cttccctgtg ttttcagaac cgctaatttc tcttcgggaa tggttaattt
     9361 aagcgtttcc gtaacgaatt tatcgccatc gatcgtgcca cgaataaaat tcatcgccgg
     9421 tgatccaata aatccggcaa caaacatatt cgctggttgg ttataaaccg ttttcggcgc
     9481 accaacttgc tgaacaatcc catccttcat aatcacaatc cgcgtcgcca tggtcatcgc
     9541 ttcggtctga tcgtgggtca cgtagatcat ggtggtattc agcttctggt gtagcttgct
     9601 gatctctgcg cgcatttgca cgcgcagctt ggcatcaagg ttagagagcg gttcatccat
     9661 taaaaacacg cccgcttcgc gcacaatcgc ccgcccgagc gccacacgct ggcgttgccc
     9721 gccggaaagc gcgcccggct tgcgtttcag gtactcacgc aggccgagaa tttgcgccgc
     9781 ccagttcacc cgctcatcaa tcacctcttt ggcgattttt tgcatcttca ggccaaacgc
     9841 catgttgtcg taaaccgtca tatgcggata caacgcgtag ttctggaaca ccatcgctat
     9901 attgcgtgct ttggctggaa cgtcattcat tcgtttgccg tcgatcaaca gatcgccgcc
     9961 gctgatctcc tcaagcccgg caatcatgcg cagggtggtc gacttaccgc agcccgacgg
    10021 gccgacaaac acgatgaact ctttatcggc aatttccagg ttgaagtcct tcaccacatg
    10081 cacctggtta tcgtagattt tttgaatatg ttgtaacgaa agctgagcca tgttgattcc
    10141 tttgctatac gttttgccag aaggccgata accgcgacca ggtcagagag tccgttgaag
    10201 gcaacagtaa ttgcgccccg gttaagcccg cgccgatccc caccgagcgc ataccgctgg
    10261 cgttaatggc gtcaatgccc gcctgcgcat cttcaatgcc gatacatgcc tgcggcggca
    10321 cgcccagccc ggcacaggcg gcgagaaaga tttccgggtc cggtttcgag tttttaagtt
    10381 gggaagcatc cgcgcagaag gtgaaaaact cgcgcagctc cagcgccgct aaaatcgtcg
    10441 gcgcattcag ggagacagaa gccagcccaa ccgggatctg ctgtgcacgg agatctgcca
    10501 gcaaagagcg aatgccgggt agaacagcgc tgaccgtcaa ctcgcgcagt gagtggacat
    10561 agagcagatt tttgcgatac gccagttgcg ccctctcctg cgagttaaag tcgccctctt
    10621 tgcccccgtg ttgcagaatg cgccgcagag actcatcgcg gctgatccct tttagggatt
    10681 cgttaaactg cgcatcaatg ctgatgccaa tttcagcggc aatctgctgc cacgcctgga
    10741 aatgcagatg cgcggtatcg gtgattacac catccagatc gaaaattacc ccttgcagtt
    10801 tcattcatcc tcctgatgtg tggtagcggt cccattgaag ggcaaaataa aatcacccaa
    10861 acagaaaaca gattcttcag ccacggatat aagctgaccg ttcaaacgca gtgaaacggg
    10921 cgcagaagtt cgaatcgcaa tacgctgcgc gtcaagagtg acctgtaatt cgcagccctg
    10981 ccagaacaga gggaaagaca actgttgcca ctgctcaggt aacgccggat tgagatgcaa
    11041 ttcaccgtca cgcacgctca ccccggcaaa accctgaatc gcccccagcc agatagcgcc
    11101 agttgcggca gcatggatac catcatcaca actatgcgga tcagcaccaa gatcgatttc
    11161 agtcccctcg cgccagaact gataactttg ggtcagcagg ccacagcgtg cggcaacaat
    11221 gccgtggatt gctttactta atgacgagtc gtgaatagtg cgcggttcat aaaattgcag
    11281 attggcaaga cacgatgccg ctgagaactg ctccggcagc atgtaattga gcatcaccac
    11341 atcagcttgt ttgaggattt gcatctcgtt cacttctgcg cgtgaataat ccagcagaat
    11401 ggtttgcttc cccgccgccg ctttgtattt cgccaggtta atcgccggct tagccataaa
    11461 cgaatcatcc tgcggcaaaa cgccgtcggg ctgaatttct ggcagccgca gttctttaag
    11521 gaacatttcg gcacgatgaa taaacgcgtc gtcgctacag ccaaactggc gggcaatact
    11581 cagcgcctgc tggacgttgt aatacgccat atagctggtg aaggcgttat tattgacatg
    11641 ttcggtatat tcgtcaggcc caataacatc atgaatttcc agacggtcgt taaccctcac
    11701 cgcgcggcta atccagaact ttgcagtttc cagaagtagc gccatgcctt catgcgcaat
    11761 gaaactttca tcccccgtgg tctgccagta ttgaataacc gcccaggcga tatcggccac
    11821 cagatgatgt tccgcctgcg ccgaggccac tttttgccgc aatgctgctg cgctcgtcgt
    11881 gggcaggcgt catgatgcgc agatgataaa gcgcataatc taacgcttgc tgatcgtgcg
    11941 cttcgccgcc atttaccgtg atacgacgtt tctgccacca ttgacgccag ttttctgttg
    12001 atactgcaag aagttggtcg taactctgct gcgcgcacat ttcaagctgg cgaagcgacg
    12061 cgctgcccca ctcgtctaaa acggcttgcc tgtcatcccg ccagtcgatc cacaccagtt
    12121 tttgcaacgt cactgtctcg cctgcatgaa gctgcgcact ggtatgttgc tgcaaacggc
    12181 gctctttggc ggtatagcat tgctgcacat caccgctcac catacagcaa cagctgatgg
    12241 ccacatcact gcgtccatcc tgggtggtgt agatcccctg catcagatgc tgaccaaaca
    12301 cccgcacctg ggtttcgtcg agatgttggc gaccgtggtt ggtttgcgtg gcgtcgatac
    12361 ctgttgaaat cagcactgac gcgtcggcgt ccagtggcgt aatagtgatt tccagcacaa
    12421 tgagcggcag ttggtctgcc gaaacaaagc gacggctggc gatggtgtaa cctgtgccgt
    12481 tgctggtacg ccagacaacg ctacggcgta attctccact ggcaaagtca agttcacgct
    12541 gccaggcttc gcgggataac gagaaaacct caccatttat ggcaatctcc atccccaaga
    12601 tatcaggcag gttcaccagt tcgttgattt caccttttcc cgcccgatga tacagccccg
    12661 ccagatacat ccctcgcgtc tggcgcgtgt aatcttcttc atggctggcg cgaagcccaa
    12721 gatagccgtt cccctgcgcc atcagcgatg catacttgtt cagggtatgc tggctgaaat
    12781 ggggttctga taacgttact ggcctggtca taattccaca cgcgtccctg tttctgcgga
    12841 ttgatacaac gccgccacca gttgctggat gatgtacccc tgctcggcgt cggctatcat
    12901 cacgggcttg ccctgtacgt gattgataaa ggcttccatg ctgcgcaaat ggcggttgtc
    12961 gtctgccagt tcccgttgca tcagcgtcat taattcaccg ttgttatcgg tgtagatatg
    13021 tgctggaaac agcgtcgcac cggctttatc accacaaaag ctgacgttca taatcgactg
    13081 ttcgcggatg ttgagtgcaa atgacgtttc cagccacaga atgccgccgt tatgaaattc
    13141 aatggtgcca aacagcgaat cttcgacgct ataagttgcc ggatcccact cgccaaattg
    13201 accacagctc ttttgcgtgc cgatcttttg aaagctatgc gcattcacgc ttttcaccgc
    13261 cggaaagccc agcacataca tcgcagcatc cagcatatga atgccgatgt cgatcagcgg
    13321 gccaccaccc tgcagttctt tattggtaaa gacgccccag ccgggaacgc cgcagcgacg
    13381 cagggcgcgg gcggtggtaa cgtaaatctc tcccaaaacg ccgttggtca cctgttcacg
    13441 cagctgttgc gtatcgagcg caaaacgatg gtgaaagtcg taggccagca ccttgcccag
    13501 tttgcgcgcg gtatcgcaca tttcccgcgc ctgttctggc gtcatggcgg gcggtttttc
    13561 gcacatcaca tggcagcccg cttccagtgc catcagggta tgttcgtaat gaaaacggtt
    13621 aggtgagcag acgctaacca catcaggttt caccgccagc agcatggcct gcggatcgtc
    13681 ccacacggat gcattcccgt atttttctgc cagcgcctgc gcctgggaaa ggcggctgtc
    13741 acagacagcc accagttcca gatcgttgcg ggtgcagtag tacgaagcat gaaccttatc
    13801 cgccacctgg cctgcgccta ttatcgcgac ccgcagtgga gagcttgtca ttgcactttt
    13861 cacaaagacc tcttagcagg tacgcaacca ggtcaacgaa tcacggtacg cctgggcagg
    13921 atcttctgcc cggatacgcc cttcatacac cacataaccc tgatagttat cggcgcgcag
    13981 ctgttcaaac agcgcgtgga aatcgagggt gccgctgccc ggctggtaac gatggttatc
    14041 ggcaatatgc acatgaccga gcaggtcgcg gttgtcgtgc agcgcctgcg ccaggttatc
    14101 ttcttcgata ttcatgtgat agaaatcgcc gataatctgc acatgtttaa gatcgttttc
    14161 gacgatgtaa cggcgggcat cggcgagagt gttgatcata tgatcctgat agcggtttaa
    14221 cggttcgaga tacaccacgg ttccggtacg cgctgccact tgttccagcg tgcgcaggga
    14281 atcgctcacc attttacgat cgccgtccag gctgcgcggc gaggtcatcg gcggtaagcg
    14341 gaaggtaaac atgccccacg ctgccggaac gacgatacct ttacccccca cttctgccag
    14401 cgcttcaaga atgcgttcga tctgctttaa gccattaaga cgacgctctt cgataaagtc
    14461 accaatccag ccgtcatagc caccgcaggc ggtggtcacc ggtaaaccgg tttctttgat
    14521 tgctgctttg acttcttcga gattgttgac cagcagtttg ccgtcaatct caaaaccatc
    14581 gaagcccatc tctttgatat aacgaaattt ctccagaatg ttttccggaa agaatgcctg
    14641 attctgtgtg cctattttca ttttttcatt cctttgaatt aaaacgtaac gcccattttg
    14701 atgctctgtt ccggatgctg gtcgacatac tgcatgtagc tttccgggct attggcaaag
    14761 gtcaccaccg ggtcgattag atcttcacaa ttgagataac cgttcatcag cagttcccaa
    14821 caggtttctt cgatacgctt gcggctccag cgcggataat ccgggttcgg ttcgctgcac
    14881 gcgcgggaga agacaatttt ggcgttattg aaatgggctt cgcgtccgag gttaaaacct
    14941 tcggcaaacg gtttggcaaa cgcaacatac gagatggtgc cgccataagc caggccgcgg
    15001 agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga taatcacatc ggcaccctgc
    15061 ttgccggtca gcgttttgat ctctttacct acgtcagtgc caatggggtt aaggcagaaa
    15121 tcagcaccgt ggcgacgggc aatatcacag cgataggcga taggatcgac gccaatcacc
    15181 accgacgcgc cagcgcgttt cgccagttgg atggctattt gaccaatcgc gccaagccct
    15241 accaccacca caaaatcacc cacgcgcacg ttggcatcgc gcacgccgct catggcaaac
    15301 tgcgccgggt cgtaacagac ggcgtttttc caggagctgc cttgcggcat tttgcgcagc
    15361 ttgtagttat tcaccgcgtt aatgatgacc gtctcggaga gtgggccgta gccgcatacg
    15421 ctgtcgccca ccgcgtagtc ggttacgtcg ctgccgcact cgataatgtc gccaaccacc
    15481 atgttgccaa gctggaattt gccaaactca atgccgcgcg gcgcatctgc cggacgcgga
    15541 gtgaacatct gccattcacc gttaaagtct tcatcgataa acgggctggc ggcgcggaag
    15601 tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca cgcggatctt aacttcattg
    15661 gctaaaatcg cccgatcttc atactcaacc agcgcagcaa cgcgcggtgc tgtggctact
    15721 aactttttca tttaaaactc cttgtaaaga aactggccgc tttagccctt cacgccaccg
    15781 gcggtcaaac cactcttaat aaaacgttcc gaaagcgcat acataatcac caccggcaat
    15841 gcggtcacca gtgaggccgc catcatccgt ccccagatgt aatctggcgt actgaacagt
    15901 gcgttcaggc ccaccggtaa ggtgaaattg ctggcgctgg aaaggaaaat cgacgcaaac
    15961 agatagtcgt tccacgccac cataaagcag tagacgaaca cggatatcag cccggacatc
    16021 gccaggggca cggtgatacg gaagatgatt tgcagacggt tgaggccatc catcatcgcc
    16081 gcttcttcga tctcatcagg gatggtgtcg aagtagcttt tcagcataaa cacggcggtg
    16141 ggcagggttt gcgtcaccat ggtaatgatc agcgccatct cggtgtcgta aatgcccagc
    16201 gcggtgatga ttttgaacag cggcaccacc agcaaaatgc cagagaacat atacaccgtg
    16261 taaaagctgg cattgatggt catccgacct ttaaagcgca ggcgggaaag cgcgtaagcc
    16321 ccaagaatgc cgaggaatac cgccaccacg gatgaaacca ccgacaccac cagactgtta
    16381 cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa tgtcgacgta atgctccagc
    16441 gtccactgct gcggcagcag cgtaggatgc agtgagatcg cctctttcgc gcccttgaac
    16501 gaggtcatca gcatcacaaa aaatggaaac agcgtgatga tgagaaacag cgccagcccg
    16561 cagtaaaaac cgatgcgact gagtgtgcgt ttatttgttg ccattgaggt tcacccgttt
    16621 cctggtcagc agaatgacag cgaaaataat gatgaagagc accaccgaga tcgccgccgc
    16681 ttttcctaaa tcattaaagg caaaggcggt tttgtagaga tatacaccga gaatatcgac
    16741 cttggtcgtc agcaaataaa catccgcgaa catgtagaac atccagatag tgcgcagggt
    16801 caccaccgtc gccaggaccg gcataatcgc gggcagcgtg acgatacgaa accgttgcca
    16861 ggcattagcg ccatccatct ctgccgcttc gtacagcgat ttgtcgatgg tctgcaaaat
    16921 cgccagaaac gagataaagg catacgggaa gtagcgccag atggcgaaca gcaccaccag
    16981 cacaaagcta ctgcccggat tatcgaacca cagcggtgcc tgctcataga gatgcagaag
    17041 atcgacgccg aggtagttca caatgccgta gccgttgttg aacatgtatt tccaggcaaa
    17101 caccagcgaa atggacggcg ttacgtagga gaggatcacc agcgaacgcg cggttttgcg
    17161 cagacggaac tcgcggttga aaaacatcgc cactgccagc ccgagcacgg tgctgcccgc
    17221 caccaccagc gcggtatacc agaccgtcat ccacagcgag tgccagaagc cgggatcgga
    17281 gaggatacgc acatagttac tcaccccaac aaaggttgac tcgatgttgg gattgagcgg
    17341 cagacgtaaa aaactgattt cgatattcga caccattggc caggccacca gaccacccag
    17401 cagtaataag ctgggcgcga gaagcagcag cgcaaagggc atatcggaac gacctgaaaa
    17461 aagcctgttc attgcggatt cctgtcctgg gtttagtgct gttcgatcag ttcatccagc
    17521 tttttctggc tcgcttgcag cgtgccaggg agatcggctt tacccacggt gacgttatgc
    17581 accatggagc tcaccacgcc agaccccgtc acatcaccca tgcgggtaaa gtttttatcc
    17641 cctactgcac caaagacctg aatatttggc agttcaccga ttagctgatt cggtagttca
    17701 cccagcgcct taataacgtc gttgtctttc caggtggcgg tagtgacaac cgctttattc
    17761 accggcagcg cagcacctgg cgacatcatc acccagtcgg caatgttgtc tgcctgctcc
    17821 ataaaggtga caaatttttc tgctgcttcc gtctcttcgg tcttttgccc ggcggtaatg
    17881 gtcagcgagg tcaacatgcc gtagaccgca gagtttttct cggttggcac cacgaaaccg
    17941 acgtttttcg ggtcgccttc tttaatcaca gccggaagga tataggtgga gtaaatcgcc
    18001 atcggcacgg tgccgttcat aaaggcgtct ttcacttcca tgatgtcgtt agaacccggc
    18061 atagtgttgg cagcaaggtc gcggtaatag gtcagtgcct gcatcatctc tggtgtatca
    18121 agggtgattt tgccttcggc gttaaagacg ttagcctggt tggataacgc aaactgggag
    18181 aaggattgtt ccgtcaacac gctttctgct gtaggcagcg caatgccgta ttttttattc
    18241 gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt gccagttttt cggctcctca
    18301 agccccgctt ttgccagcac atctttgcga taccagatac cgccaatcca ggcgctgaca
    18361 ggaacaccgg tccatgcgct accatcttcg gtacgcacaa tacgcagtac gccatcgtaa
    18421 aacgcgcctt caccaacgtt gctgatgact gtggcaaccg ctttgcgatc gataagctgc
    18481 tctttgtcca tcactttggc gtagtcatgg ctggtttcta tcacttccgg cagcgaaccg
    18541 ctacgtgaaa gagtaatgac tttagtgtta taggcatctt cttccacggg cacttgctta
    18601 acggtgatgc cagggttttc tttttcaaaa cgggcaatca atttactgat aacggcctgg
    18661 cgctcctgct ccaccgaaga atgcataaat tcgatggata cattcgtttt attttcttct
    18721 ttacagcctg aaatcagggc gcaggaaacc agtgctgata acagcacgat ttttgattta
    18781 atcatgagat gttccttttt atttaatcca cataacctgc caggggcgca gcgttatttc
    18841 attacccaat atatcaactt cactaatcag atcccgacca tgcagattag taatatttac
    18901 atgctgaata tttttactga cattaaacaa tccagtcaga caattaccat cagcgttact
    18961 tctttgaata cgcattacgg atgaattaat cgtatcgatg gtaaaattat tatccggatg
    19021 aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat ataccgcatg
    19081 ccttaatgta gcttcatcgt tcagttctcg ggttatctct ttactgtgat attttttacg
    19141 gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac gcgagccaag
    19201 aatactttga atatatatcg ctggcacacc ggggaaactt aacaaaatcg catgggcgag
    19261 aataaacctg gcgcaacgtt cttcatcgct actctcacgg cggcttaacg catccatata
    19321 ggtcacattt atttcatacg gactgcgtgt accgtcggga ttatttttcc agtttactaa
    19381 tgcaccttcc tgctgtaacg cctcgaccag ctctaatatt tcgctttcag gcaacaagcc
    19441 ccgtagcggg tttagcccga tgccatcgtg agaggcgagg aagttaaacc aggtggtgtt
    19501 gctggaaggt agtgtcaggt tttgcgccca cgcacaaagc gcctcaacgt tctgtttttg
    19561 caccgcatgc agcaccagtg gcggcagcga gaactggtac accatatgtg cttcgtcatc
    19621 gcctgcgcca aagtaagcaa tgttgtcttt atgcggaaca ttggtctcgg taatgatcac
    19681 tgtacctggc gcaacgttat caataatcga ccgtaacagt ttgataatca gatgtgtttt
    19741 ttccagatgg atgcagcttt ttcccggctc tttccacata aagccaacgg catccaggcg
    19801 gacatactca gcacctttcg caaggtaaca gagtaaaaca tccaccatcg ccagcaacac
    19861 ttcagggcta cggtaattca ggtcaatttg atcgtcacta aaggtggtcc ataaatggcg
    19921 cgttgaatga tcgcgcatct ggaatggcgt taataacggt aacgcacgcg ggcgagtgac
    19981 ggcgctgaga tcggtttgcg ggtcaacggc aataaaaaaa tcttcaaaac ctggatgctg
    20041 ttgtaaatag tttttaaacc attcactttt tgccgacata tggttgcaga caaaatcaaa
    20101 cattaaatga ctgcattcac cgagttgctg aatatcctgc cactcccccg cttcactggc
    20161 gacctgatga taatcaatta ccgaaaagcc atcatcagat gaccatggat aaaacggcaa
    20221 caaatgaaca tgtgaaaaaa tgctttgcag ccattgatgg taaaactgat taaatgtggg
    20281 taatggtttt aaatcattgc tgtgaaattg atcggcatag gtaattaaca cgacatcact
    20341 ttcatcccag tgttttttac gtcgctgtgt aattaatcgt ttcgcactct caagacgcgt
    20401 tacaagtttc tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt ccaggtaatc
    20461 cgtaattttc tgtttcatgt tgcctttccg tggtagcggt cccatgaaac gagagactac
    20521 tccgcagaaa ataaactgtc aattgagcac aagggtttta gtcacgcagg aaatcatttt
    20581 ttaccaggga aaaagcgtat ttgtgagctg acgcaaataa tctatgggga tgtaaataag
    20641 gtgtgacaga aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt ttaacctttg
    20701 accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt atatcccatc
    20761 tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata cactttcacg
    20821 gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt caggggaata
    20881 ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacgaac atcgatccag
    20941 tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa gcctttttta
    21001 aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct gagatttaac
    21061 aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta gcagcccgac
    21121 ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga gcaactgatt
    21181 ttatcgccca gcccgctaca cccgccgggc catagcgcag tgcggtaaga agtaccagct
    21241 tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag cgagtattca
    21301 taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg aagtgacata
    21361 acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga cttcatccag
    21421 caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca ttggatcaag
    21481 cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga agaaaatcga
    21541 cagcaccacc aggatacgca ccagttttgc cggtacatca aaatagttgg caatcccggc
    21601 gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat tgagattaat
    21661 gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga ttccagcgcc
    21721 tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg ctgctgctca
    21781 ctttgcgaca attcactgcg accagaacga ttgctgtaat gcagccataa ccagatcggt
    21841 aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct cataagtact
    21901 ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat tgattgtctt
    21961 gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg gctttcagtt
    22021 cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct gcttccgctt
    22081 ccatctggtc aatacgacgt tcgaaagatt cgaaacgagc cattgcttca tccagtttgc
    22141 cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga cgtaacatca
    22201 atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcaccg atctctttct
    22261 tcatgcgtgc cagcgtgtcg tccaccagcg tcacttcatg ttccaggctc ttaatcagat
    22321 cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc tctttcagca
    22381 gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt tcaatacggc
    22441 gagtcagctg tttcttttct gccaacgcgc gcgccgaagt agaacgtact tcaaccagtg
    22501 tatcttccat ctcctggatc atcagacgaa ccagtttctg tggatcttcc gctttctcta
    22561 acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata cccataatgt
    22621 tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca atttgcgtgc
    22681 caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc aacatgccag
    22741 gatgagttag cgaattacac taacaagtgg cgaatttcat catggcagaa tacaaagata
    22801 atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg catctcgcac
    22861 cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag ctgattgcca
    22921 gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt aactgcgcgg
    22981 cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg gcgtttaccg
    23041 gtgcgcaaaa acgtcatcct gggagatttg aacgtgccga cggcggtacg ctatttcttg
    23101 atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgtgtg attgagtacg
    23161 gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg ttggtatgcg
    23221 cgacgaatgc cgatctcccg gcgatggtca atgaaggcac ttttcgcgct gacctgctcg
    23281 accgactggc ttttgatgtt gtacaactgc caccactgcg cgagcgcgaa agcgacataa
    23341 tgttgatggc agaacacttt gccatccaga tgtgtcggga aatcaagctg cctctgttcc
    23401 cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg ggaaatattc
    23461 gtgaattgaa aaacgtggtg gaacgttcag tgtatcgcca cggcaccagc gattatccgc
    23521 ttgatgacat cattattgat ccctttaaac ggcgtccgcc tgaagacgct atcgccgttt
    23581 cagaaaccac ctcgcttcca acactgccgc tggatttacg tgagtttcag atgcagcagg
    23641 aaaaagagtt gctgcaactc agtttgcaac aagggaaata taaccagaag cgcgcggctg
    23701 aattactggg gttaacttat catcagttcc gcgcgttgtt gaaaaagcac cagatttagc
    23761 gaagttgagc aatacaactt gcaggatgcg gcgtgaatac tttattcggc atacataatc
    23821 aatacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg ttgggctatt
    23881 aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcaca tccgggatgg
    23941 tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc aggagcagcc
    24001 cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc ttgcccgttt
    24061 gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca gcaatggccg
    24121 gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt tgattcgcgc
    24181 gttcgcagag tgattctttg tcgataatat tgagcaccgc gtgcgcggca gccaccgcca
    24241 gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc ataatattcg
    24301 cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc atcgtcatta
    24361 agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc gcaaagccgc
    24421 tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg cgaatggcgg
    24481 caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt tcgaaaataa
    24541 tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg gcgtcgaggg
    24601 agtcctgtgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac accgaaccag
    24661 ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc atggtcatat
    24721 atgtacgacc gtggaagcca ccgctaaacg caatcacgcc aggtcgtccg gtatgggcgc
    24781 gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaat gcggttttag
    24841 cctgcccgct cactggggca agggcgttaa ttttctccgc cagcgtgacg tagctttcgt
    24901 acggcacaat ctgatacgcc gtgtgggtaa actgttgcaa ttgctgctcc accgccgcga
    24961 ccagatcagg gtggcgatgc ccggtattca gcaccgcaat gcctgcggcg aaatcgatgt
    25021 attcgttgcc ctctacgtcc ttcagcgtag cgttttctgc cgactgggcg aagaagttac
    25081 acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat tcattgttgc
    25141 tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc cagacgatcg
    25201 cgcaggctgt aataagccgc gcccatcgcg gtaaatggca cgcgcagcgt gcgcccgccg
    25261 gggaacgggt aatgcggcag attggcgaag gcatcgaaac gttcggcgtc gccgcgcagc
    25321 agttcggcaa tcagacgccc ggcaagatgg gtacaggtga cgccgtggcc gctgtagccc
    25381 tgcatgtaat agatgttggt atcaaggcga ccgaactgcg gcatgcgcga caaggtcagc
    25441 aggaagttgc ccgtccagcg gtaatcaatt ttcacgccct tcagctgcgg gaaggttttc
    25501 agcagtttcg gcaccacaag gcgctcaacg tcatccgggt cgcgcgcgcc gtagaccacg
    25561 ccgccgccgt acagcaggcg gttgtcggcg gtaagacggt agtaatcaag cagatagtta
    25621 cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc cgacagccgt
    25681 tcggtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc cggctctact
    25741 ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac ctgacctttg
    25801 gcagttcgca caacggctgg cgtggtgtgc tggatctgcg tcacggcaga aagttcatac
    25861 acgcgcccgc cgttgaggcg gatggcgtcc gcttcgccaa tggcgaggtt tagcggatga
    25921 atatgcccac cgctgtgatc cagcagcgca ccggtatagc gatcgctggc tacttcacgg
    25981 cgaatggcgt tggcgtccag caattccagc tgtttattgc cgtagcgttc ccagttctct
    26041 ttctgctctt caagtgtggc gagctgttta tcattcatcg ccacaaacag gccgccgggg
    26101 cggtagtcgc agtcaatctg ataacgtttg atacgttcgc ggatgatctc accaccttcg
    26161 aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc gatcacgtcg
    26221 atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc gccaaagccg
    26281 atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg cagcgcggaa
    26341 gagagcccgg tatagccgcc gccgaccacg caaacgtcgc aggaaatcga ctcattcagc
    26401 gtgtcgaatg gcgcgtattt attcgcactg gcggcgtagt aactgctggt atgttcggtc
    26461 atttcaggcc tccaggctta tccagatggt tttcagttcg gtgaattttt caagggcatg
    26521 cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca cggtcatatc
    26581 gccgtcgttg tagttattga cgaagacgga accagccttc aggcggcggc tcatgcggtg
    26641 cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc tgtcgttggc
    26701 aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg gaccgaaaat
    26761 ctcttcgcgg cttaaggacg cattcgggtc tacatccaca aagatggtcg ggccgatggc
    26821 ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt cgccttcccg
    26881 aataaagcta tggaccgagt cggcgtgggc gcagtcgatt aaggtgccca tggtggttgc
    26941 gggatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca gggctaagaa
    27001 ttcatcggcg atgctctctt ccagcaacaa acgcgttccg gcgatgcaca cctggccctg
    27061 gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg gacaatcggc
    27121 gaaaacgagg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca tgttgctgtc
    27181 gcccgcatct ttcagcagct gtttcccggt acgggttgaa ccggtaaagg caatggcgtc
    27241 gatatcgtta tgacgcgaca gcgcctgccc cgcttcatga ccaaaacccg tcaccacgtt
    27301 caacacacca tccggcaagc ctgcttcttt cgccagcccc gcgagacgaa tcgcactgag
    27361 cggtgatttt tcagacggtt ttagaatcac gctgtttccc gccgccagcg ccgggccgag
    27421 tttccagcaa gtcagcaaca gcgggaagtt ccacggcacg atggcggcaa tcacgccgac
    27481 cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt cgccatacac
    27541 tttgtcgatc gcttcggcgt accagcgaat ggcgcgcgcc gcgccgggaa tatcatcacg
    27601 cagactgtga cgaatcggtt tgccggtgtc gagagtttcc agcagtgcca gctcttcggc
    27661 gtgggcttcc attaaatcgg cgagtttatt cagtaccgct ttacgtttag ccggagaaga
    27721 cagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac ggtcgatatc
    27781 gacgctcttg ccgcgggcaa ttttcgccag cggtgcctgg gtgaccggat caacggtttc
    27841 aaaggtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc ggttttcaat
    27901 ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca ttatgactcc
    27961 tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag atccccgggc
    28021 cgtatgcccg tttatttctg gcgacattct ttatatacac ttcatccttc aagccgcctc
    28081 tgccttggct gcgttcgctc ccccgaatca cttacctgag taagctcatc gggattcgct
    28141 cacttgccgc cttgatgcaa cttgaatgat tttgtgtata gattgccggg cgcattgcgc
    28201 ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg ctgataattc
    28261 ggcaaatacc tgccgacgta ttactgaaac tgtgcggtat gccggtatta atggcataac
    28321 tttgccccgc gacgaggtgg taatcctgac cattaatcgt cagaacaatt tcaccttcca
    28381 gtactgtgcc tatttcctca ccctgatgct taattctttc cccagtggtt gtgcccggct
    28441 ggtacgtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga accagcttca
    28501 ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg acctgcggct
    28561 catcaggttt ttccggctcg gaaaagaatt ccgagagtga cagaccatac accttcagca
    28621 gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta ctgatagcac
    28681 tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc tgctggcgga
    28741 tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca tttccttaaa
    28801 ctgtagtggt cagagtcgct gtttttcagc gatatggtgc tgacaagcgg tgataaagcc
    28861 ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt gccactgtac
    28921 gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat ccggcgagcg
    28981 agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgcccatgca gagagtttac
    29041 ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt caacctgaac
    29101 ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc gatgttccag
    29161 caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca ccaccagttc
    29221 ctgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg cattaattaa
    29281 cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt tttcaccata
    29341 taggtgcggc tgcacattgc tgggactacc aggaagataa atgccatcga gtttcggcaa
    29401 aagttgttca agtaatgacg gttccgccag cgcatgtggt agcgcaattg gcaagccgcc
    29461 tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat gacccttaag
    29521 cctgttcctg cacattacga caccgataac cggattgttc attatatttt ccatgctcaa
    29581 tctcacaaag tggactaaat tatcgccatt actgcggttt aaatgacctt tatgttcaat
    29641 attttttcaa tctagcagtg gtttttcatt tttgcaaacc caatttaaca tttgacaaac
    29701 atttagtttg catacagatt cgaatggtgg tcattatatt ttacgctttg ataacgagcg
    29761 gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag agaactttgt
    29821 tcagcagtca gaagagaggc ggggtagcgc ctttacgcag gaagtgaaac gctacctgga
    29881 gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg gttgcttccg
    29941 tggaaaacgt atccctgttt caagcctgaa gaagttggag aaagggtgtt atttcccggc
    30001 ctcggtgttt gcaatggata ttctgggcaa cgtagtagaa gaagcgggtc tgggtcagga
    30061 aatgggcgag ccggatcgta cttgtgttcc tgttctcggt tccttaaccc cttctgccgc
    30121 cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg gcgctccctt
    30181 tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctacgcc agcgcggatt
    30241 gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgtcagc gcgacgctga
    30301 agggtatctg caaccgccct gcgcgcccgg caccgatgac cgcaatacac aaagccaggt
    30361 ttactccgtt gataacctca accacttcgc cgatgtgctc aatgatattg atgaactggc
    30421 gcagttacag ttaattcccg ccgacggtgc ggtcgccgaa gcctcgccgg gtcagtttga
    30481 aatcaacctt taccataccg ataacgtgct ggaagcctgc gatgatgcgc tggcgctaaa
    30541 acgcctggtg cgtctgatgg cagaaaagca taagatgcac gccactttta tggcgaagcc
    30601 gtatgaagag cacgcgggca gcggaatgca tatccatatc agtatgcaaa ataaccgtgg
    30661 cgagaacgtg ctttctgacg cggaaggcga agattcgccg ctgctgaaaa agatgctcgc
    30721 cgggatgatt gacctgatgc cgtcatcaat ggcgttgctg gcaccaaacg tgaactcgta
    30781 tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc ataacaaccg
    30841 caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg aatatcgcgt
    30901 ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg gtattttgca
    30961 tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc tggaacagga
    31021 aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg agaatgatca
    31081 cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta aaaatgatga
    31141 gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt tgaaaaacgc
    31201 gtaattcccc atgcggggtt gtttcatcgg cctgagcgga gccaggcctg acatttgctg
    31261 gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg ggcttccgtt
    31321 aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc ccgcatggcg
    31381 agggtggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct ttgcttccct
    31441 tgctaaacag gtttgatttc aggttgacac gaccgcaaac cttattacgc aggggaggca
    31501 gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa aacccgtctg
    31561 cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct cacgccgatg
    31621 accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt tccggcgtcc
    31681 tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa actggttcgc
    31741 cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa tccgcacgtc
    31801 ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat gatcaacgtt
    31861 ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg ggtgtgggtg
    31921 gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct ggtcgctaac
    31981 ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat cttcctggtg
    32041 gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca gccgtttatc
    32101 agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg tttctcgttc
    32161 ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc acgcgtgatc
    32221 ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc ggcgtcgttc
    32281 tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc cgcactgcct
    32341 gaaattgcgc tgtacgtcgg cggtaagctg ttccagtcga ttttcctttg caccacgttt
    32401 gtgaacacgt tagcgtctgg cctggcctca catgccagcg tgtcacgtct gctgtatgtg
    32461 atggggcgcg acaatgtgtt tccggagcgc gtgtttggct atgtgcatcc gaaatggcgg
    32521 actccggcac tgaacgtcat tatggtcggg attgtcgcga tgtcggcgct gttcttcgat
    32581 ttagttaccg ccacagcgtt aattaacttc ggcgcgttgg tagcgttaac ctgtcggtgt
    32641 tcaaccactt ctggcggcgc aaaggaatga ataaaagctg gaaggaacac ttccactatt
    32701 tattaatgcc gctggttggc gcgctgacgg tgggcgtgct gtggattaac ctcgaagcca
    32761 cctcgctgac gctgggtctg gtatgggctt cgttaggcgg cgcatatttg tggtatctga
    32821 tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag aacgccagtg agcgaaacgt
    32881 aatagtcgga tgcgcgccgc attatcctgc ctgagggtaa tgcgccgcgc cattaactat
    32941 aaaaagatgt atgacccgga tcacgagtgc tatcatccgc ccttatgctt agcgcattaa
    33001 ccccaggggc aaataatgaa ccacgatatc ccgctaaaat attttgatat tgccgatgag
    33061 tacgcgaccg aatgcgcaga gcctgtcgca gacgctgaac gcacaccgct ggcccactac
    33121 ttccagttac tgctcacccg tttgatgaac aatgaagaga tcagcgaaga agcccaacat
    33181 gaaatggctg ctgaagcggg gattaatccc gtacgcattg atgagatcgc agagtttttg
    33241 aatcaatggg gtaatgagta attatttaat tgcagacgat cggtcagtat cgttgtccgg
    33301 ataaagcgtt cacgccgcat ccggctaaca atgacacgca ctgcagcgtg ctttaatggg
    33361 tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt ctgtgagtta tagttataga
    33421 gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac ctgttcaacg accaaccaca
    33481 cggccctcat tacgacccct gaacacaaac ggtaatggca aatgtcatta aatattagta
    33541 agatagcctc tttacataaa atcccttaat attatgcgcc aggtattatc gtctcttttg
    33601 gtgattgctg gacttgtgag tggtcaggca atcgccgcgc ctgaatcccc cccgcatgct
    33661 gatatccgcg acagcggttt tgtctattgc gtcagcgggc aagtcaacac ctttaaccca
    33721 tccaaagcga gcagtgggtt aattgtcgat acccttgccg cccagtttta tgatcgactg
    33781 ctggatgtcg atccctatac ctatcgcctg atgccggaac ttgccgaaag ctgggaagta
    33841 ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg atgttccgtt tcaaaaaacc
    33901 gactggttta ctcccactcg taaaatgaat gccgacgatg tggtgtttac cttccagcga
    33961 atttttgacc gcaacaaccc gtggcataac gtcaacggca gcaacttccc ctacttcgac
    34021 agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac tggataatca taccgttgag
    34081 ttccgactcg cccagccgga tgcttctttt ttgtggcacc tcgcaaccca ttatgcttcg
    34141 gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag atcgccagga gcaactcgac
    34201 cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat accgcgccgg gcaatttatt
    34261 cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt taatgccgca ggtagtggtg
    34321 gatttaggct ccggcggcac cggacgtctg tcgaaactcc tgaccgggga atgcgacgtt
    34381 ctggcctggc ctgctgccag ccagctatcc attttgcgtg acgacccgcg cttgcgttta
    34441 acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta acaccgccaa accgccgcta
    34501 aataatcccg ctgtccgcca tgcactggca ctggcgatta ataaccagcg cctgatgcaa
    34561 tccatctatt atggtacggc tgaaacggcg gcctctattt taccgcgcgc ctcgtgggcc
    34621 tatgacaacg aggctaaaat tactgaatac aatccggcga aatcgcgcga acagttgaag
    34681 gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc ccacacgttc gcaggcgtgg
    34741 aaccccagtc cactgaaaac tgccgaactg attcaggcgg atatggcgca ggttggcgta
    34801 aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg cgcggttgat ggatatgagc
    34861 catgatctga cgttatccgg ttgggcgacg gacagtaacg acccggacag tttcttccgt
    34921 ccgttactga gctgcgcggc aattcattca cagaccaacc tcgcccactg gtgcgatccg
    34981 aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc agctggcagc gcgtattgaa
    35041 gcctatgacg aagcgcagag tattctggcg caggaattac ctattctgcc gctggcgtcg
    35101 tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc tggtacttag cccgtttggt
    35161 aacgcctcct ttgctggggt gtatcgcgag aaacaggatg aggtgaaaaa accatgatta
    35221 tcttcacctt acgccgcatt ttgttattga ttgtcacctt gttcctgctg accttcgttg
    35281 gcttcagttt gagctatttc actccacacg cgccgttaca aggcgcgtca ctgtggaatg
    35341 cctgggtgtt ctggtttaac ggcctgatcc actgggattt tggtgtttcc agcattaacg
    35401 gtcaaccgat tgccgaacag ttaaaagagg tcttccctgc gacgatggaa ctgtgcatcc
    35461 tcgccttcgg ttttgcgctg attgttggca tccccgtggg gatgattgcc gggattacgc
    35521 gccataagtg gcaggataac ttgatcaacg ccatcgcctt gctgggcttt tcaatcccgg
    35581 tgttctggct ggcgcttctg ttgacgctgt tttgttcact cacgctaggt tggttgcccg
    35641 tttcagggcg tttcgatctg ctgtacgaag tgaaaccgat taccgggttt gcgttgattg
    35701 atgcctggct ttcggactca ccatggcggg atgaaatgat catgagcgca atccgccata
    35761 tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac tgaagtgatc cgactgatgc
    35821 gtatcagcac tatcgaagtg tatgaccaga actatgttaa agcggcggct acccgcggtt
    35881 tgtcgcgctt tactattttg cgtcgccacg ttctgcataa cgcgttaccg ccggttattc
    35941 ctcgcctggg cttacagttt tccaccatgc tgacgctggc gatgatcacc gaaatggttt
    36001 ttagctggcc gggtttaggg cgctggttaa ttaacgccat ccgccagcag gattacgcag
    36061 ccatttccgc cggagtgatg gtgtgtggct cactggtcat tattgttaac gtgatttctg
    36121 atattttggg tgccatggct aaccctctga aacataagga atggtatgcc ttacgatagc
    36181 gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg cctggcgcaa attttatagt
    36241 gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc tggctgtact gtgtattttt
    36301 ggcggctggt ttgcgcctta cggcatcgac cagcaatttc tcggttacca attattgccg
    36361 ccgtcatggt cgcgctatgg cgaagtttct ttcttcctgg ggactgacga cctcggacgc
    36421 gatgttttaa gccgtttact gagcggagct gcgccaaccg tgggtggcgc atttgtggtg
    36481 acgcttgccg cgacgatctg tggcctggta ctgggcacct tcgccggggc gacgcacggc
    36541 ctacgctcag cggtgcttaa ccatattctg gataccttac tggcgatccc ttcgctgctg
    36601 ctggcaatta tcgttgtagc gtttgccgga ccgagtttgt cccacgctat gtttgctgtc
    36661 tggctggcat tgctgccgcg tatggtacgt tcgatttaca gcatggtgca tgacgaactg
    36721 gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat caacgctgaa tattctctgg
    36781 tttgctgtga tgccaaacat caccgccggg ctggtcaccg agatcacccg cgcactgtcg
    36841 atggcaattc tcgatatcgc cgcgctgggc tttctcgatc tcggcgcaca actcccctcg
    36901 cctgaatggg gagcgatgct cggtgatgcg ctggaactga tttatgtcgc cccgtggact
    36961 gtcatgctgt caggtgcggc aattatgatt agcgtgttgt tggttaacct ccttggcgat
    37021 ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc attactcgat attcgtaacc
    37081 tgaccattga atttaaaacc ggtgatgagt gggttaaagc cgtcgaccgg gtaagtatga
    37141 ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc cggttccggc aagagtttga
    37201 ttgcgaaagc gatttgtggg gtgaataaag ataactggcg tgttactgct gaccgtatgc
    37261 gttttgatga tatcgatttg ctgcgtctct ccgcacgcga acggcgcaaa ctggttggcc
    37321 ataacgtgtc gatgattttc caggaaccgc agtcgtgtct tgacccttca gaacgtgtgg
    37381 gtcgccagtt gatgcaaaac atcccagcct ggacctataa aggccgttgg tggcagcgtt
    37441 ttggctggcg caaacgccgt gccattgaac tgctgcaccg cgtggggatt aaagatcaca
    37501 aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg tgaatgtcag aaagtgatga
    37561 tagccattgc actggcgaat caaccgcgtc tgctgattgc tgacgaaccg accaactcaa
    37621 tggagccaac aacccaggcg caaatctttc gcctgctgac gcgtctcaac caaaacagca
    37681 ataccactat tttgcttatc agccatgact tacaaatgct tagccaatgg gcggataaaa
    37741 ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc gagtaaggag ttggtgacga
    37801 tgccacatca tccttatacc caggcgctga tccgcgcgat accagacttc ggcagcgcga
    37861 tgccgcataa aagtcgcctc aatacgctgc ccggcgctat cccactgctg gaacagttac
    37921 cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca acgagaatgc attgtgacgc
    37981 cacgtttgac gggggcgaaa aatcatctct atgcctgtca tttcccgctg aacatggaga
    38041 aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt taagtaaaac gttccgctac
    38101 cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa aacccttgag ctttacgcta
    38161 cgtgaaggcc agacactggc gattattggc gagaatggtt cgggtaaatc cacgctggca
    38221 aaaatgctgg cgggaatgat agagcctacc agcggcgagt tattgattga cgatcatcca
    38281 ctgcattttg gcgattattc cttccgcagt cagcgtattc gcatgatttt tcaggatcct
    38341 tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc tggattttcc actgcgcctg
    38401 aacaccgacc tggaaccgga acagcgtcgc aaacagatta ttgaaacgat gcgtatggtt
    38461 gggctgctac cggatcacgt cagttactat ccgcatatgt tggcacccgg acaaaaacag
    38521 cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag tcattattgc cgatgaagcc
    38581 ctcgcttcac tggatatgtc gatgcgttcg cagttgatta atctgatgct ggagttacag
    38641 gaaaaacagg gcatttcgta tatttatgtt acccagcata tcggaatgat gaagcacatt
    38701 agcgaccagg tgctggtgat gcatcagggc gaggttgtcg agcgaggcag caccgcggat
    38761 gtgctggcat caccgctgca tgaactcacc aaacggctga ttgccgggca ctttggcgaa
    38821 gcattgacag cggatgcgtg gcgtaaagat cgctaatttt ctgaatcctc cttccagatt
    38881 attcacctca ttctgacaag agattaaatc ttgtcagaat gaggtgatgg ataaaataaa
    38941 atcaaatgcg cgtgatttaa gaagcaatct cactttgcag gaacgaaagc tctggcgata
    39001 tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt caacatccgg tggggagcta
    39061 cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt gagctggatg gtgggcagca
    39121 tgatttggca gttgcctatg ataccaggcg cactagctgg cttgagtcgc agggctggac
    39181 cgtgctgcgt ttctggcata acgagattga ttgtaatgag gaggcggtgc tggagattat
    39241 tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag ggcgaggggg ccgaccgagc
    39301 cgaatagctg ttgtggtgaa aacatggaga cagtgctgga gaatattcgg caaggtctga
    39361 accatcccag ccatcgccat aaaagggtta ggggctgtat gagcctgttt gttgctgggg
    39421 taacaatatt tgcacaatat ggtcccctcg cccctctggg gagagggtta gggtaagggg
    39481 aaaggcgccc accctgccgc agcctgctcc ggtcggacct ggcaactata gctactcaca
    39541 gccaggttga ttataataac cgtttatctg ttcgtactgt ttactaaaac gacgaatcgc
    39601 ctgattttca ggcacaacaa gcatcaacaa taaggattaa agctatgggt tttctttccg
    39661 gtaagcgcat tctggtaacc ggcgttgcca gcaaactatc catcgcctac ggtatcgctc
    39721 aggcgatgca ccgcgaagga gctgaactgg cattcaccta ccagaacgac aaactgaaag
    39781 gccgcgtaga agaatttgcc gctcaattgg gttctgacat cgttctgcag tgcgatgttg
    39841 cagaagatgc cagcatcgac accatgttcg ctgaactggg gaaagtttgg ccgaaatttg
    39901 acggtttcgt acactctatt ggttttgcac ctggcgatca gctggatggt gactatgtta
    39961 acgccgttac ccgtgaaggc ttcaaaattg cccacgacat cagctcctac agcttcgttg
    40021 caatggcaaa agcttgccgc tccatgctga atccgggttc tgccctgctg accctttcct
    40081 accttggcgc tgagcgcgct atcccgaact acaacgttat gggtctggca aaagcgtctc
    40141 tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc ggaaggtgtg cgtgttaacg
    40201 ccatctctgc tggtccgatc cgtactctgg cggcctccgg tatcaaagac ttccgcaaaa
    40261 tgctggctca ttgcgaagcc gttaccccga ttcgccgtac cgttactatt gaagatgtgg
    40321 gtaactccgc ggcattcctg tgctccgatc tctctgccgg tatctccggt gaagtggtcc
    40381 acgttgacgg cggtttcagc atcgctgcaa tgaacgaact cgaattgaaa taatcattct
    40441 gttggtaaat attgggcggc gttctgccgc ccgttatctc tgttatacct ttctgatatt
    40501 tgttatcgcc gatccgtctt tctccccttc ccgccttgcg tcaggataac gatttccttt
    40561 acgaccaagg agcgcccatg gaacaacgcc acatcaccgg caaaagccac tggtatcatg
    40621 aaacgcaatc cagtactgcg gagtatgacg ttctgcctct ggtcccggaa gccgcaaagg
    40681 tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga aacgctggct cccttccttt
    40741 catggctggt ccctgcgcgc gttcttgcag tggaattgtt ccctgaccag cttaccgtaa
    40801 cccgttcaca gaccttcacc gcttatgaac gcttgtctac ggccctgacg gttgctcagg
    40861 tttgcggcgt ccagcggtta tgtaactact attcggcgcg acttacgccg ctccccgggc
    40921 ctgattcctc cagggaaagc aatcataggt tggcacaaat cacgcaatat gcccgccaac
    40981 tggctagctc gccttctatt atcgacaacc gatcgcgcca gcatctgaat gacgtcggtc
    41041 ttactgccgg ggactgtgtg atcattaacc aaatcattgg ttttattggc tttcaggcgc
    41101 ggaccattgc gacatttcag gcttatctcg ggcacccggt acgctggtta cccggtctgg
    41161 aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga atcaatacgc tggcgaagca
    41221 gttatgaggt ggaaaaacta cctgaagagt acacaaaaag ttcaacagca gaactttgcc
    41281 aactggccga aacactctct ctccacccta tttcactttc ccttctcgaa aggttgttaa
    41341 acagtacacg ggttaataca cagccggata ataagcttgc ggcgttgtta tgcgcgcgga
    41401 taaatggcag tcctgcttgt tttgccgcct gtatggattc atcaaatgaa tataaaaaaa
    41461 tcagccccct tctgcgcaag ggcgaaaatg aaattaacca atgggctgac cgtcattctg
    41521 ttgagcacgc taccgttcag gcgatacaat ggctgacccg agcacccgat cgctttagcg
    41581 ccgcccagtt cagcccttta ctcgaacacg aaaaatcatc aacgcagatt attaatctgc
    41641 tggtatggag cgggctgtgt ggctggataa atcgcttaaa aatcgcgttg ggtgagacat
    41701 attaaccttg ccgcgtcaga cagattcgcg taaaactgtc agccgctcta atggccacca
    41761 aaatagacaa ttatgtttca ggacaacccg ctgctagcgc agcttaaaca gcaactgcat
    41821 tcccagacgc cacgcgctga aggggtggta aaagccacag aaaaaggctt tggcttcctg
    41881 gaagtcgacg cgcaaaaaag ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat
    41941 ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac gtgaatccgc agagccagaa
    42001 gaactggttg aaccgttcct gactcgtttc gtgggtaagg ttcagggcaa aaatgaccgt
    42061 ctggccatcg ttcctgatca tcctctctta aaagacgcca ttccttgccg cgcagcccgt
    42121 ggcctgaacc acgagtttaa agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg
    42181 ctgaaaggcg atcgttcttt ctatgcagaa ctgacacaat acatcacttt tggtgacgac
    42241 cactttgtac cgtggtgggt tacccttgca cgccataatc tggaaaaaga agcaccagac
    42301 ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg aagatctgac cgcgctggat
    42361 tttgtcacca tcgacagtgc cagcacagaa gatatggatg acgccctttt cgctaaggcg
    42421 ttgccggatg acaaacttca gctgattgtg gcgattgccg atccaaccgc gtggattgct
    42481 gaaggcagta agctggacaa agccgcgaaa attcgcgcat tcaccaacta tctgcctggc
    42541 ttcaacatcc ctatgctgcc tcgcgagctt tctgacgatc tctgctcact gcgcgccaat
    42601 gaagtccgcc cggtactggc atgccgcatg acgctctccg ctgatggcac cattgaagat
    42661 aatatcgaat tctttgccgc caccatcgaa tccaaagcga agctggtgta tgaccaggtt
    42721 tctgactggc tggaaaatac cggtgactgg aagcctgaaa gtgaagcaat tgccgaacaa
    42781 gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt ggcgtcataa ccacgcattg
    42841 gtgtttaaag atcgcccgga ttaccgcttt attctcggtg aaaaaggtga agtgctggat
    42901 atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg aagaagcgat gattgccgct
    42961 aacatttgtg cggcccgcgt actgcgcgat aagctcggtt ttggcatcta taacgtgcat
    43021 atgggctttg atccggcgaa tgccgacgcg ctggcagcgt tgctgaaaac gcacggtctg
    43081 catgtcgatg ccgaagaagt gctcacgctg gacggtttct gcaaactgcg tcgtgaactg
    43141 gatgcgcaac caactggttt cctcgacagc cgcattcgtc gcttccagtc atttgctgaa
    43201 attagcactg aacccggtcc tcactttggc ctcggtctgg aagcatacgc cacctggact
    43261 tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa
    43321 ggcgaaactg cgacgcgccc acaggatgaa atcactgtcc aaatggccga gcgtcgccgt
    43381 ctcaaccgga tggcagaacg tgatgttggc gactggttat acgcacgctt cctgaaagac
    43441 aaagccggga ccgacacccg tttcgcggcg gaaattgtcg atatcagccg tggcggcatg
    43501 cgtgtgcgtt tggttgataa cggcgctatc gcctttattc cggcaccttt cttacacgct
    43561 atgcgcgatg aactggtttg cagccaggaa aacggcactg tacaaattaa aggtgaaacg
    43621 gtttacaaag taactgacgt tattgacgtc accattgccg aagtccgcat ggaaacccgc
    43681 agcattattg cgcgcccggt cgcgtaatct cctttcacgg cccattcctc atggatgggc
    43741 cgtttatttc cccgctctac cttcgttata tccaggcaga ttttttaact gactttcgtt
    43801 tgaaaactgg cgtttttcca aaactggatt acaaataaat atataaaaaa aacaatgcgt
    43861 ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg cggactccgc
    43921 tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt tgtacaactt
    43981 tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgttt tgcgcttttc
    44041 taccaccgaa accacagaac ctgatcgtac attgcagtta tctgccgaac aggctgctcg
    44101 catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg tcgatgaaag
    44161 cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg ctggcaacgc
    44221 gtctgcatgg catgacacac cggcggttgc tcgtgattta tcacacgggc tttcctttgc
    44281 tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc gggggaatat
    44341 ccaacgcttc aatcggttat gtgaagatta cacagggttg aaagaacacg acgtcattgg
    44401 gcaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca ggcgcaataa
    44461 ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac caacatgtaa
    44521 aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg gcaaaaacga
    44581 gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc aggagcgact
    44641 gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg caatgcagga
    44701 tttaatcgat cacgctatta atcatgcaga taacaataaa gttggggttg tgtatcttga
    44761 tttggataat ttcaaaaagg tcaacgacgc ctatgggcat ttgtttggtg accagttatt
    44821 acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtgt tggcgcgtcc
    44881 aggtggggat gagtttctgg tactggcatc caacacctca caaagcgcgc tggaagcaat
    44941 ggcatcacga attttgaccc gcttacggct cccctttcgc attggtttaa ttgaagttta
    45001 taccagctgt tcagtaggta ttgcactctc tcccgaacat ggttcagaca gcacggctat
    45061 tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag gacaattttg
    45121 cgtttttacc ccagaaatga atcaacgggt atttgaatat ctctggctgg ataccaactt
    45181 gcgtaaagca ctggaaaacg atcagttggt tattcactat caaccgaaaa tcacctggcg
    45241 tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac gtgggttgat
    45301 tccaccgttg gacttcattt cctacgccga agagtcaggg ctaattgtgc ctttaggccg
    45361 ttgggtgatt ctcgatgtcg tacgccaggt ggcaaagtgg cgggataaag gcataaacct
    45421 gcgagtggcg gtaaatattt ctgcacgtca gctcgccgat caaaccattt tcaccgccct
    45481 gaaacaggtt ctccaggaac tcaattttga atactgccct atagatgttg aactgacaga
    45541 gagttgtctg attgagaatg atgaactggc actgtctgtt attcaacaat ttagccaact
    45601 aggtgcgcaa gtgcatctgg atgattttgg taccggctac tcttcacttt cgcaactggc
    45661 gcgctttccg atcgatgcca tcaaacttga ccaggttttt gttcgagata ttcacaaaca
    45721 acctgtctcg cagtcactgg tccgggcgat cgtcgctgtg gcccaggcat tgaatcttca
    45781 ggtgatcgcc gaaggtgtag agagtgcaaa ggaagatgct tttttaacca agaacgggat
    45841 caatgagcgg caaggatttt tgtttgccaa accgatgccc gccgtcgcct tcgaacgctg
    45901 gtataaacgc tatctgaagc gcgcataagt agatgaatat atatacttag cgctatatcc
    45961 cgtcattata tatcgtattc ctggatgata attagccgtt gctgttaaac acttctcttt
    46021 tagcggcaat ttttgcatta ttcattttcc acttttgcta taaggatgtc cttgatgtct
    46081 gaatttgatg cccagcgcgt tgccgaacgt attgatattg tgttagatat ccttgtggca
    46141 ggcgattacc actctgcgat ccataatctc gagatactta aagcagaact cttgcgccag
    46201 gttgctgaat ctacgccaga catcccgaag gcaccgtggg agatttaaca atcgtgcctt
    46261 caggttttcc tatttgagga tgcccggcgc gtcagtattt ttttatttag tattataacg
    46321 ttataagaat tacagcgatg aattcccgac aacaaactat tctacagatg gtcattgacc
    46381 agggtcaggt tagcgtaacc gatctggcaa aagccactgg agtttctgaa gttaccattc
    46441 gccaggatct caacaccctc gaaaaactga gttacctccg ccgtgcacat ggctttgcag
    46501 tttcgcttga tagtgatgac gtcgaaaccc gtatgatgag caactatacg ctgaagcgtg
    46561 aactcgccga gtttgccgcg tctctggttc aaccgggcga aaccatcttt atcgaaaatg
    46621 gtagcagcaa tgccctgctc gcccggactc tgggcgaaca gaagaaaaat gtcactatca
    46681 tcacagtcag cagctacatt gcgcatttgc tgaaagacgc gccttgtgaa gtcattttgc
    46741 tcggtggcgt gtaccagaaa aaaagcgaaa gtatggttgg ccctttgaca cgccagtgca
    46801 tccagcaggt acatttcagc aaagcattta ttggtattga tggctggcaa cctgaaactg
    46861 gattcaccgg tcgcgatatg atgcgtaccg atgtggtcaa tgccgtgctg gaaaaagagt
    46921 gcgaagcgat agtcctgact gacagctcga aatttggtgc tgtacattca tactccatcg
    46981 gtcccgttga gcgattcaat cgcgtgatta ccgattcgaa aatacgcgcc agcgatctga
    47041 tgcatcttga gcacagtaaa ctcaccgttc acgtcgttga catttaacca ttccccttct
    47101 ctacggatga tttgcagttt ggcaaatcat ccgctctaag atgattcctg gttgataatt
    47161 aagactattt acctgttatt aacacgctta agatataaaa ttattatcag cgatataaca
    47221 ggaagtcatt atcacctgcg tgatataacc ctgcgcgcga gcagatttca cggaataatt
    47281 tcaccaggct tattcttagc tattatagtt atagagagct tacttccgtg aatcataaat
    47341 tcaggagaga gtattatgtt tgtaacgagc aaaaaaatga ccgcggctgt tctggcaatt
    47401 actttggcaa tgtctctgag tgcctgttct aactggtcta aacgggaccg caacaccgca
    47461 atcggcgcgg gtgcaggggc attaggcggt gcagtactga ccgatggcag tacgttgggt
    47521 acattaggtg gtgcagctgt cggtggtgtt attggtcacc aggtcggtaa ataatcgtaa
    47581 ataaatgtcg attatcgact cgcaattata atacgcttga ttctgttcag tacgaaataa
    47641 ctgaataata cgcacagtaa aatattcgaa agccacggat atatccgtgg ctttttatgt
    47701 taaccgcctg cgagttttac cttcatccct ttcgcttcaa gtagtgattt taataaatca
    47761 cgcttatcgc cctggatttc aataactcca tctttaactg ctccgccgca gccgcatttt
    47821 ttcttcagtt ccgctgcaag ttttgtcagt tcggcatcat cgagatcgac accggtaatc
    47881 aggcaaacgc ccttaccttt acgtccactg gtctgacgct gaatacgcac cacaccgtcg
    47941 cctttagggc gtacaggggc cgctttgggc tcatcaatac gtccggtttc cgttgagtag
    48001 accagacggc tgttggaatc actcatcatg cactccgctg taaagaggcg ttgatcgctt
    48061 tcagcgtctg cgctggatct accgattgcg ttaccgggcg accaatcacc atataatcaa
    48121 caccagccgc caacgcctgt tctggcgtca taatgcggcg ctggtcacca gcatcactcc
    48181 cctgcggacg aatgcccggt gtaaccagtt tgaactcctg accgaatacc tgtttaaagc
    48241 gcacagcttc ctgagcagaa cacaccacac catcaaggcc acatttttgc gtcagtgccg
    48301 ccagacgttc tgcataatct gcaggtgaca gtgtcacgcc aagatcggcc aggtcgctag
    48361 cttccatgct ggtcaacact gtcacagcaa tcaaaagcgg tgcatctttg ccaaacggaa
    48421 ccagtgcctc acgcgctgcg gtcatcatac gcgccccacc agaggcatga acattcacca
    48481 tccacacgcc taagtcagct gcagcagcga cagcgtgcgc tgcagtgtta ggaatatcgt
    48541 ggaatttcag gtcaagaaag atatcaaaac cacgctgttg aagttcgcgc acaaactgtg
    48601 gcccaaacaa tgtaaacatc tctttgccga ccttcagacg acaatcgcgt gggtcgatct
    48661 tgtcgacaaa ggacagcgcg tcatcacgat tatgataatc aagggcaaca accacaggag
    48721 aattcgtaac agcgcgggaa gaagatgaag cagttaacgt catgaccaga ccttcttgat
    48781 gatgggcacc ggaagtggcg cgaaacaggt aaacggcgag cattctaccc gtggaaataa
    48841 aaaattaaca gttgcgattt ttcccctggc aggctttttc tgctccgtcg ttgccggagc
    48901 acaatggaac atcaataact agtatgttgt aactaaagtc ggattttttt aaaaattaca
    48961 ggccatcaag accgcgaatc ggtttaatgg ttgaccaggc ccgacaagac ggacaatgcc
    49021 agtagagggt gtatgcggta aaaccacatt tctggcagcg ataacgaggc ttactacgta
    49081 ccttttcgcc aaccatgtca cgcagcacca tcaggctctc tttggcacgc ccttcttccg
    49141 cttcatttaa gtggtaatcc attaacttat ggaacacacg catggtcgga tgacgctgaa
    49201 gctggcgcgt aatatagacc tgtgcggcct cactaccgtc gcgcgcttcg atgatatcag
    49261 caagcatcaa ttcagcatcg gcaccggtgt tctcttccac cgcgcgttgc aggaattctg
    49321 cccattcggc agttttaccc aactgctgat agcaggtttg caacatttcc agcgtttcgc
    49381 tgaccagttc tctgtcctgg gatatcacgc gttgcaaact ttcgacggct ttggcgtatt
    49441 ctcctttcgc cataaacacg cgtcccatca tgatggatac gcgggcgctg tttttatctg
    49501 ccgccgcccc tttttttagc aaggtcatgg cacgatcgag atcgtcgctg gccatatgct
    49561 gcagggctaa ctcacagtag aaatgggcaa tttcgacgcg ctgtttatct ttacccagct
    49621 tcaccaggcg ttcggcaaca tcaattgctt tctgccactc gctggtagcc tggtagattt
    49681 gtagcaactg ttgcagcgcg ccaatgcgga agtcagtttc atcggtcagc tgattgaaca
    49741 tgtcttccgc gcggtcgtat aacccggcgg ccatgtaatc acgccccagt tgttgaatcg
    49801 ccaacagacg ctgttcatag gtcagtgagg cgctttccat tagggtctga tggatgcgaa
    49861 tagcgcgatc aacttcgcca cgcgaacgga acaggtttcc gagcgtaagg tgggcttcaa
    49921 cggtgcctgt atcctcttta agcatatcga gaaacaggtc taccgcttta tcctgttgat
    49981 tactaagcag gaagttaacc cccgctacgt aatcacgcga caagcggttg gcttcatctt
    50041 gcttgttttg ttgcgcactt ctgcggccca tataccagcc ataggcagcg gctacaggca
    50101 aaagcagaaa caacaactcc agcatagaaa gttattcctt cgccgctgac gagtgcggca
    50161 ctacagccac gtcagtcgcg ggtgaaagct ggttttccag tcgctttatt ttacgttcag
    50221 cgcgcgccag ggaaacacga actcgcagcc agaacaggcc acaaatcaac caaccgatag
    50281 caaaccccgc agcaaacaat accgccaaca atgtggaaat acggtactcc ccttgcgcta
    50341 acagataatt aaacgtcacc tgttgatcgt tctgcgcacc caacgtgacc gaaatcacga
    50401 agatcgctaa caccagtaag aaaatgagta aatatttcac attacttccc gttatgtggt
    50461 tagagcgaat aaattatggt caacttaccg caatagccct ttaaagtacc attttcgcgc
    50521 cgtctgcgaa atggcatcca cgcgcattgt tgatgattta agggctaaat tcggaaaatc
    50581 aaccagcgtt aactttcttg ttctcgttgc gctatttcgc gattttcttc cgcaggtggt
    50641 gttaatggcc cacaaattcg ttgcgcaagc cacgttgcca ccgccaccag cgcccacgaa
    50701 atcaacgtag ctactaccag atcgcgtggc caatgcatcc cgagcagcag gcggcttccc
    50761 atgactcccg ttgcccagac cagcaagata gcaatggtta acgttcgccg acgcggccac
    50821 agcaaaccaa cggccagcag tgcccaactg gcagcaaaca tcgtgtgacc ggaaggaaag
    50881 gcaaaccccg tctctttctg ccagtgtgaa cgcaaatatt gtgggatatt tttctcttca
    50941 gccaactgtt ctttcactaa atttccgcgt tctgctcgct ttaaagtgta gaactcatca
    51001 accggaatat gatgtgtttt ttccagccag ataacaaaag gtcgtggttc ctggactttg
    51061 tctttgatcc aggatttaac gccttgtccc acaaggattg cggccgccag aatggcaaat
    51121 aatacaaagg cagccttaat gcgaaaacgc agacaccaga gaaaccagcc gaataaaatc
    51181 aaatgtgtaa tgacgcccca gggctgggtg acagtttcag taacccaaaa agccgctttt
    51241 agtagccaac tttgttctcc aggttgccaa cgccagccag aaatccatac ggctactggc
    51301 atgacaagca atagtgcagc tcccactgcg gtacgtctgg caatcgaacg catggcctct
    51361 cctttttgat aagtcccaca atcataactg aaaacgccag ttccaggaaa aattgacaga
    51421 tttgtgccat tccgtgaacg atcgacgcgt cgtgattagg tgaacccctt ctcgttatgg
    51481 caaaataagc caatacagaa ccagcattat ctggagaatt tcatgcagct taaacgtgtg
    51541 gcagaagcca aactgccaac cccatggggc gatttcctga tggtgggatt tgaagaactg
    51601 gcaaccggac acgatcatgt cgcgctagtc tatggcgata tttccgggca taccccggta
    51661 cttgcgcgcg tccattccga atgtctgacc ggtgacgccc tgttcagctt gcgctgcgat
    51721 tgtggcttcc agctcgaagc ggcattgacg caaattgccg aggaaggccg tggtattttg
    51781 ctgtatcacc gtcaggaagg tcgtaacatt ggtctgctga ataaaatccg cgcttacgca
    51841 ctgcaggatc aaggttacga taccgtagag gctaaccacc agttaggctt cgccgctgat
    51901 gagcgcgact tcactctttg cgctgatatg ttcaaactcc ttggcgtcaa tgaagtccgc
    51961 ttgttaacca ataacccgaa aaaagtcgaa attctgaccg aagcagggat taatattgtt
    52021 gaacgcgtac cattgattgt aggtcgtaac cccaataacg aacattatct cgataccaaa
    52081 gccgagaaaa tgggccattt gctgaacaaa taaccctctt gcattgtgta attagtttgc
    52141 ttgccggaag caaaataacc ggcaagcaaa tacttgttac ttcaacatat tacgaatgac
    52201 ataatgcaaa atgccgtcgt tctggtagta ggtcaactcc gtcgcggtgt cgatacgaca
    52261 acggcagggt acgacctcct ggctaccatc cgcgcgcgta agcgtcaccg gaaccgtcgc
    52321 accgggttgt agattttgca gatcgccaat atcaatcttc tcttccccgg ttagtcctaa
    52381 cgttttacgc gttacgcctt gcggaaattc cagcggcagg atgcccatgc caattaaatt
    52441 cgaacggtga attcgttcaa acgattcggc aatcaccaca cgaattccaa gcagacgagg
    52501 accttttgcc gcccagtcac ggcttgagcc tgatccatac tctttcccgg caatcaccgc
    52561 cagcggcgtt tgttcctgct tatagcgcat cgcagcatca taaatagaga ctacgtcgct
    52621 gtcaggtaaa tgccgcgtca tccccccttc aacgccaggc accatttcat tacggatgcg
    52681 aatattggcg aaggtgccgc gcatcatcac ttcatggtta ccacgccgcg aaccgtagga
    52741 gttaaagtct tttcgctcaa caccccgacc ttgtagatat cgacccgctg ggctgtcggg
    52801 cttaatactg cccgccggag agatatggtc agtggtgact gaatccccca gcattgcgag
    52861 gatccgcgca ccgtgaatat cttccactgg tgctggtgtt gcctgcattt catcaaagaa
    52921 aggcgataag cgaatatagg ttgagtcctc ctgccaaccg taggtatcgg atcgtgtgac
    52981 gttaatttcc ttccactctg ctgtgccttc aaaaacttct gcgtactctt tgcggaacat
    53041 ttctgtggag acttgttcta ccgcacgggc aatttcttgt gccgatggcc agatatcttt
    53101 cagatatacc ggatcgcctt tacgatcatg gccgataggc tcagaagcca ggttgatatt
    53161 catatttccc gccagcgcat aggcaaccac cagcggcggc gaggccagcc agttggtttt
    53221 aaccagcgga tggatacggc cttcaaagtt acggttgccg gacagcactg caccgacggt
    53281 taaatcgcct tttttgattg ccgtttcgat aggatcgggc agcggcccag agttaccaat
    53341 acaggtggta caaccgtatc ccacaaggtt aaaccccagt tcgtcgagat acggtgtcaa
    53401 tttcgctttt gccagataat cagaaacaac tttcgagcct ggtgccagcg acgctttgac
    53461 ccacggttgc cgcttgaggc caagagttac ggcttttttc gccagcaagc ctgcagccat
    53521 cagcacactt gggttagagg tgttggtgca cgaggttatc gcagcaatga ccacagcgcc
    53581 atcaggtaac tgatactgat gtccgttcat aacataatcg accggctggc gatctttatg
    53641 cgtggcattc acttccagtt cgttactggc ggcaaatgct tttggtacat cgggcagtgc
    53701 aacgcgatcc tgtgggcgtt ttggccctgc caggctcgct tcaacgtcat tcatatccag
    53761 ttctaacgta ctggtaaaaa tcggttcatc gcccgggtta cgccacatgc cctgcgcttt
    53821 ggcatatttt tcgaccaact caacctgatc ttcgctgcgc ccgcttaaac gcatgtaatc
    53881 gagggttaca gcatcgattg ggaagaagcc acaggtggca ccatattctg gcgacatatt
    53941 ggcaatggtg gcgcgatccg ccagcggtag tgaatccaga ccatcaccat aaaattcgac
    54001 gaatttcccc accacgccat gtttgcgcag catttgggta acagtgagaa ccaggtctgt
    54061 ggcggtaata ccttcacgta atttaccggt aagtttaaag cccactacgt ccgggataag
    54121 catggaaacc ggctggcc
//
LOCUS       DAZFVO020000011        53274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFVO020000011 DAZFVO020000000
VERSION     DAZFVO020000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 53274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.011"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(108..1193)
                     /gene="yhfS"
                     /locus_tag="ACHKNB_RS02915"
                     /old_locus_tag="pgaptmp_000583"
     CDS             complement(108..1193)
                     /gene="yhfS"
                     /locus_tag="ACHKNB_RS02915"
                     /old_locus_tag="pgaptmp_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4194159.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1205..2509)
                     /gene="yhfT"
                     /locus_tag="ACHKNB_RS02920"
                     /old_locus_tag="pgaptmp_000584"
     CDS             complement(1205..2509)
                     /gene="yhfT"
                     /locus_tag="ACHKNB_RS02920"
                     /old_locus_tag="pgaptmp_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HGU4194160.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2521..2874)
                     /gene="yhfU"
                     /locus_tag="ACHKNB_RS02925"
                     /old_locus_tag="pgaptmp_000585"
     CDS             complement(2521..2874)
                     /gene="yhfU"
                     /locus_tag="ACHKNB_RS02925"
                     /old_locus_tag="pgaptmp_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HGU4194161.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(2885..3763)
                     /gene="php"
                     /locus_tag="ACHKNB_RS02930"
                     /old_locus_tag="pgaptmp_000586"
     CDS             complement(2885..3763)
                     /gene="php"
                     /locus_tag="ACHKNB_RS02930"
                     /old_locus_tag="pgaptmp_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HGU4194162.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3760..4986)
                     /gene="yhfW"
                     /locus_tag="ACHKNB_RS02935"
                     /old_locus_tag="pgaptmp_000587"
     CDS             complement(3760..4986)
                     /gene="yhfW"
                     /locus_tag="ACHKNB_RS02935"
                     /old_locus_tag="pgaptmp_000587"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4194163.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLLVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(4986..6149)
                     /gene="yhfX"
                     /locus_tag="ACHKNB_RS02940"
                     /old_locus_tag="pgaptmp_000588"
     CDS             complement(4986..6149)
                     /gene="yhfX"
                     /locus_tag="ACHKNB_RS02940"
                     /old_locus_tag="pgaptmp_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HGU4194164.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(6233..6595)
                     /gene="yhfY"
                     /locus_tag="ACHKNB_RS02945"
                     /old_locus_tag="pgaptmp_000589"
     CDS             complement(6233..6595)
                     /gene="yhfY"
                     /locus_tag="ACHKNB_RS02945"
                     /old_locus_tag="pgaptmp_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGU4194165.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6612..7517)
                     /gene="yhfZ"
                     /locus_tag="ACHKNB_RS02950"
                     /old_locus_tag="pgaptmp_000590"
     CDS             complement(6612..7517)
                     /gene="yhfZ"
                     /locus_tag="ACHKNB_RS02950"
                     /old_locus_tag="pgaptmp_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HGU4194166.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7786..8790)
                     /gene="trpS"
                     /locus_tag="ACHKNB_RS02955"
                     /old_locus_tag="pgaptmp_000591"
     CDS             complement(7786..8790)
                     /gene="trpS"
                     /locus_tag="ACHKNB_RS02955"
                     /old_locus_tag="pgaptmp_000591"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGU4194167.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8783..9541)
                     /gene="gph"
                     /locus_tag="ACHKNB_RS02960"
                     /old_locus_tag="pgaptmp_000592"
     CDS             complement(8783..9541)
                     /gene="gph"
                     /locus_tag="ACHKNB_RS02960"
                     /old_locus_tag="pgaptmp_000592"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HGU4194168.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9534..10211)
                     /gene="rpe"
                     /locus_tag="ACHKNB_RS02965"
                     /old_locus_tag="pgaptmp_000593"
     CDS             complement(9534..10211)
                     /gene="rpe"
                     /locus_tag="ACHKNB_RS02965"
                     /old_locus_tag="pgaptmp_000593"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGU4194169.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10229..11065)
                     /gene="dam"
                     /locus_tag="ACHKNB_RS02970"
                     /old_locus_tag="pgaptmp_000594"
     CDS             complement(10229..11065)
                     /gene="dam"
                     /locus_tag="ACHKNB_RS02970"
                     /old_locus_tag="pgaptmp_000594"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4194170.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11172..12458)
                     /gene="damX"
                     /locus_tag="ACHKNB_RS02975"
                     /old_locus_tag="pgaptmp_000595"
     CDS             complement(11172..12458)
                     /gene="damX"
                     /locus_tag="ACHKNB_RS02975"
                     /old_locus_tag="pgaptmp_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HGU4194171.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12550..13638)
                     /gene="aroB"
                     /locus_tag="ACHKNB_RS02980"
                     /old_locus_tag="pgaptmp_000596"
     CDS             complement(12550..13638)
                     /gene="aroB"
                     /locus_tag="ACHKNB_RS02980"
                     /old_locus_tag="pgaptmp_000596"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGU4194172.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13695..14216)
                     /gene="aroK"
                     /locus_tag="ACHKNB_RS02985"
                     /old_locus_tag="pgaptmp_000597"
     CDS             complement(13695..14216)
                     /gene="aroK"
                     /locus_tag="ACHKNB_RS02985"
                     /old_locus_tag="pgaptmp_000597"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HGU4194173.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14617..15855)
                     /gene="hofQ"
                     /locus_tag="ACHKNB_RS02990"
                     /old_locus_tag="pgaptmp_000598"
     CDS             complement(14617..15855)
                     /gene="hofQ"
                     /locus_tag="ACHKNB_RS02990"
                     /old_locus_tag="pgaptmp_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HGU4194174.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15767..16171)
                     /gene="hofP"
                     /locus_tag="ACHKNB_RS02995"
                     /old_locus_tag="pgaptmp_000599"
     CDS             complement(15767..16171)
                     /gene="hofP"
                     /locus_tag="ACHKNB_RS02995"
                     /old_locus_tag="pgaptmp_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HGU4194175.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16161..16601)
                     /gene="hofO"
                     /locus_tag="ACHKNB_RS03000"
                     /old_locus_tag="pgaptmp_000600"
     CDS             complement(16161..16601)
                     /gene="hofO"
                     /locus_tag="ACHKNB_RS03000"
                     /old_locus_tag="pgaptmp_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HGU4194176.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16585..17124)
                     /gene="hofN"
                     /locus_tag="ACHKNB_RS03005"
                     /old_locus_tag="pgaptmp_000601"
     CDS             complement(16585..17124)
                     /gene="hofN"
                     /locus_tag="ACHKNB_RS03005"
                     /old_locus_tag="pgaptmp_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HGU4194177.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17124..17903)
                     /gene="hofM"
                     /locus_tag="ACHKNB_RS03010"
                     /old_locus_tag="pgaptmp_000602"
     CDS             complement(17124..17903)
                     /gene="hofM"
                     /locus_tag="ACHKNB_RS03010"
                     /old_locus_tag="pgaptmp_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HGU4194178.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            18023..20575
                     /gene="mrcA"
                     /locus_tag="ACHKNB_RS03015"
                     /old_locus_tag="pgaptmp_000603"
     CDS             18023..20575
                     /gene="mrcA"
                     /locus_tag="ACHKNB_RS03015"
                     /old_locus_tag="pgaptmp_000603"
                     /EC_number="2.4.99.28"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HGU4194179.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDESL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20740..21300)
                     /gene="nudE"
                     /locus_tag="ACHKNB_RS03020"
                     /old_locus_tag="pgaptmp_000604"
     CDS             complement(20740..21300)
                     /gene="nudE"
                     /locus_tag="ACHKNB_RS03020"
                     /old_locus_tag="pgaptmp_000604"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HGU4194180.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21620..23755
                     /gene="igaA"
                     /locus_tag="ACHKNB_RS03025"
                     /old_locus_tag="pgaptmp_000605"
     CDS             21620..23755
                     /gene="igaA"
                     /locus_tag="ACHKNB_RS03025"
                     /old_locus_tag="pgaptmp_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HGU4194181.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVFLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            23820..24488
                     /gene="yrfG"
                     /locus_tag="ACHKNB_RS03030"
                     /old_locus_tag="pgaptmp_000606"
     CDS             23820..24488
                     /gene="yrfG"
                     /locus_tag="ACHKNB_RS03030"
                     /old_locus_tag="pgaptmp_000606"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HGU4194182.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24499..24900
                     /gene="hslR"
                     /locus_tag="ACHKNB_RS03035"
                     /old_locus_tag="pgaptmp_000607"
     CDS             24499..24900
                     /gene="hslR"
                     /locus_tag="ACHKNB_RS03035"
                     /old_locus_tag="pgaptmp_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HGU4194183.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24925..25803
                     /gene="hslO"
                     /locus_tag="ACHKNB_RS03040"
                     /old_locus_tag="pgaptmp_000608"
     CDS             24925..25803
                     /gene="hslO"
                     /locus_tag="ACHKNB_RS03040"
                     /old_locus_tag="pgaptmp_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGU4194184.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25938..27662)
                     /gene="yhgE"
                     /locus_tag="ACHKNB_RS03045"
                     /old_locus_tag="pgaptmp_000609"
     CDS             complement(25938..27662)
                     /gene="yhgE"
                     /locus_tag="ACHKNB_RS03045"
                     /old_locus_tag="pgaptmp_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HGU4194185.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRICNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            28041..29663
                     /gene="pckA"
                     /locus_tag="ACHKNB_RS03050"
                     /old_locus_tag="pgaptmp_000610"
     CDS             28041..29663
                     /gene="pckA"
                     /locus_tag="ACHKNB_RS03050"
                     /old_locus_tag="pgaptmp_000610"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HGU4194186.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29740..31092)
                     /gene="envZ"
                     /locus_tag="ACHKNB_RS03055"
                     /old_locus_tag="pgaptmp_000611"
     CDS             complement(29740..31092)
                     /gene="envZ"
                     /locus_tag="ACHKNB_RS03055"
                     /old_locus_tag="pgaptmp_000611"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HGU4194187.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31089..31808)
                     /gene="ompR"
                     /locus_tag="ACHKNB_RS03060"
                     /old_locus_tag="pgaptmp_000612"
     CDS             complement(31089..31808)
                     /gene="ompR"
                     /locus_tag="ACHKNB_RS03060"
                     /old_locus_tag="pgaptmp_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HGU4194188.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32036..32512
                     /gene="greB"
                     /locus_tag="ACHKNB_RS03065"
                     /old_locus_tag="pgaptmp_000613"
     CDS             32036..32512
                     /gene="greB"
                     /locus_tag="ACHKNB_RS03065"
                     /old_locus_tag="pgaptmp_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HGU4194189.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32609..34930
                     /gene="yhgF"
                     /locus_tag="ACHKNB_RS03070"
                     /old_locus_tag="pgaptmp_000614"
     CDS             32609..34930
                     /gene="yhgF"
                     /locus_tag="ACHKNB_RS03070"
                     /old_locus_tag="pgaptmp_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGU4194190.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35368..35595
                     /gene="feoA"
                     /locus_tag="ACHKNB_RS03075"
                     /old_locus_tag="pgaptmp_000615"
     CDS             35368..35595
                     /gene="feoA"
                     /locus_tag="ACHKNB_RS03075"
                     /old_locus_tag="pgaptmp_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HGU4194191.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35612..37933
                     /gene="feoB"
                     /locus_tag="ACHKNB_RS03080"
                     /old_locus_tag="pgaptmp_000616"
     CDS             35612..37933
                     /gene="feoB"
                     /locus_tag="ACHKNB_RS03080"
                     /old_locus_tag="pgaptmp_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HGU4194192.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            37933..38169
                     /gene="feoC"
                     /locus_tag="ACHKNB_RS03085"
                     /old_locus_tag="pgaptmp_000617"
     CDS             37933..38169
                     /gene="feoC"
                     /locus_tag="ACHKNB_RS03085"
                     /old_locus_tag="pgaptmp_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HGU4194193.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            38372..39250
                     /gene="rpnA"
                     /locus_tag="ACHKNB_RS03090"
                     /old_locus_tag="pgaptmp_000618"
     CDS             38372..39250
                     /gene="rpnA"
                     /locus_tag="ACHKNB_RS03090"
                     /old_locus_tag="pgaptmp_000618"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HGU4194194.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(39279..40049)
                     /gene="bioH"
                     /locus_tag="ACHKNB_RS03095"
                     /old_locus_tag="pgaptmp_000619"
     CDS             complement(39279..40049)
                     /gene="bioH"
                     /locus_tag="ACHKNB_RS03095"
                     /old_locus_tag="pgaptmp_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HGU4194195.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            40087..40770
                     /gene="gntX"
                     /locus_tag="ACHKNB_RS03100"
                     /old_locus_tag="pgaptmp_000620"
     CDS             40087..40770
                     /gene="gntX"
                     /locus_tag="ACHKNB_RS03100"
                     /old_locus_tag="pgaptmp_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HGU4194196.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            40829..41404
                     /gene="nfuA"
                     /locus_tag="ACHKNB_RS03105"
                     /old_locus_tag="pgaptmp_000621"
     CDS             40829..41404
                     /gene="nfuA"
                     /locus_tag="ACHKNB_RS03105"
                     /old_locus_tag="pgaptmp_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HGU4194197.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41765..43081
                     /gene="gntT"
                     /locus_tag="ACHKNB_RS03110"
                     /old_locus_tag="pgaptmp_000622"
     CDS             41765..43081
                     /gene="gntT"
                     /locus_tag="ACHKNB_RS03110"
                     /old_locus_tag="pgaptmp_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4194198.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43126..45210)
                     /gene="malQ"
                     /locus_tag="ACHKNB_RS03115"
                     /old_locus_tag="pgaptmp_000623"
     CDS             complement(43126..45210)
                     /gene="malQ"
                     /locus_tag="ACHKNB_RS03115"
                     /old_locus_tag="pgaptmp_000623"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HGU4194199.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45220..47613)
                     /gene="malP"
                     /locus_tag="ACHKNB_RS03120"
                     /old_locus_tag="pgaptmp_000624"
     CDS             complement(45220..47613)
                     /gene="malP"
                     /locus_tag="ACHKNB_RS03120"
                     /old_locus_tag="pgaptmp_000624"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HGU4194200.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            48225..50930
                     /gene="malT"
                     /locus_tag="ACHKNB_RS03125"
                     /old_locus_tag="pgaptmp_000625"
     CDS             48225..50930
                     /gene="malT"
                     /locus_tag="ACHKNB_RS03125"
                     /old_locus_tag="pgaptmp_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HGU4194201.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(50973..51989)
                     /gene="rtcA"
                     /locus_tag="ACHKNB_RS03130"
                     /old_locus_tag="pgaptmp_000626"
     CDS             complement(50973..51989)
                     /gene="rtcA"
                     /locus_tag="ACHKNB_RS03130"
                     /old_locus_tag="pgaptmp_000626"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HGU4194202.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(51993..53219)
                     /gene="rtcB"
                     /locus_tag="ACHKNB_RS03135"
                     /old_locus_tag="pgaptmp_000627"
     CDS             complement(51993..53219)
                     /gene="rtcB"
                     /locus_tag="ACHKNB_RS03135"
                     /old_locus_tag="pgaptmp_000627"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HGU4194203.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADERQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        12665 a        14728 c        13385 g        12496 t
ORIGIN      
        1 ggcgcgttga attttacatc ccgtacgttc ccctcaccct aaccctctcc ccaaaggggc
       61 gaggggaccg ttcagtacgg aatttagctt gacggtgcta tccagcatta aatactggca
      121 atactctcac gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg gttaatgcga
      181 atcgcacaat gttctgattg tggattcgcc tggcgaaacg ttccggaaag gcgataaaag
      241 agcggcggga tttcatattt cgactctgca cccaccgggt aaggcaaggc accgcgcttt
      301 tgcgcctctt ccagcactct ggcggcaatc ggctgatgaa actcgacaat caacaccttc
      361 gactgcgcat tagcaatcac cgcgcttttc acttccggca ccgcaccacc gttaagcaaa
      421 gccagcaacc gttcagatac ccctgcctgc accgcgtgca tcactggcgc aaacaccaga
      481 ccacgcaata cttccagcgc ctgtgcgccc tggatctggc taccgccgga gtaaagcgtg
      541 gcgcgaatac ggttgataac atcagcatcg ccgaccactg caccaacacc ctctggccca
      601 aatagcttga agcaggaaaa tgtcgagaca ttcgcgccgc attcacagcc gattcgcgcc
      661 accttcatca ccgcatagtt gtcatcggtt aacgctggaa cacctgccgc gcgcaacgtt
      721 gccagcacat ctgccagcac gtagctgtcc tgcggctgct ggcgcgtatg ctgcaccagc
      781 gccgcatccg gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc attgaaatca
      841 acagtaataa gcgtcagccc catctgctca ataataaccc gtgtcgtcgg gtaaacaggc
      901 gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag ccgccagccc ggcgcgaatc
      961 gcgccagtcc ccgcgccctg caccagcgcc gcagcctgtg cgtgaaatgc atcagccagc
     1021 acctgttcca cacgctgggt aacgcgcggt tgattcagtc ctggcgttaa gcctaaatca
     1081 ccgccagtaa gaaactcgct gccggggaaa tggcgacaaa tgctatccac cagcgcaaac
     1141 tgcttttgct gcgcctcaat aatcgtcagg ctttgcagag gaaacgtctt cataggtgcc
     1201 ttccttaagc cggaacgaac aggccaagcc agtaaagaac attcagcaga ataccggtga
     1261 tcattactgc cacgaccggt gccgccattt tctgtaccgg acggcccagg gattcgttga
     1321 ggaagtaaat ggcaaccgcg atagagaatc cggtataacc cgccatctta attgctgcga
     1381 aaatcgaacc gaccagcagc gccacttcca tcagcatatt catggcgtta cggatgttat
     1441 ccgacgcatt acgcaccgac ggatagcgcc ccagccattt gccgatcgaa cgaagcaata
     1501 aaacttctgc cgaaataacc actgcaccta ataccgctgc aaccatcgga ttcggcgaga
     1561 gatagcccac cgcataaaca aaggtaaagc ccgcaactgc atacacaccc gttgctaacg
     1621 cggtggtggc aatcaacggt acaaaaccca gcccgcgcat aaattctgcc agtgccgcct
     1681 ggttgatcag cgtttgcgat tgttccggcg ttacgcctgc ggagtacgct ttctccagag
     1741 tgaagatcga cacttcactg ccggcgaaaa tcttcatgct ggcaacggcg gcaatcaatg
     1801 ctcccacgat ggcgatatag ggtaagtttt tgataatccg cgacgtgcgt tcttcaaaca
     1861 ccgaaagccc gctggcatca atatcatttt catcacgatg acgcaggtcg tgagttatcg
     1921 cgatccccag cagcatcacc atgccaataa agatttcgat ggattcaggg ttaagatgtg
     1981 ggaaatagcg cacgactacc acacgggtca tcagaacaac cacggcggcg accagacttt
     2041 gcttccagcc aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa gcagaaacca
     2101 ccggcgagct taattccccg aggctaccta atacatccac cggcagcgcg gtcagcagct
     2161 ggtttactgg caacaggcaa gtaaggatca acacgcccca gatagcgcca agaccaaacg
     2221 ccatcaggct gtttatcgcc agtacgccga ggatatcggt aggaagaaag agtaaccatg
     2281 cgttgagcag cccggttttc agggtaaaag agatccccac cgaggccaca aaaccaatgc
     2341 tcagaccaaa agcgatactc cccgcctcgc gacgattcat atagccttca atcagttgcg
     2401 gcaggatcgg gcggatgccg tcatgaaaaa cagccgccga gcgatgcgcc agaagcgatg
     2461 tcatacccgt caggcacgcc accacgataa tctgaatata cagatccata gtcgtgcctt
     2521 ttatttcaga tggttaatca gcatcggaat cgcgtgttcg acgtgctcaa cggaaaggcc
     2581 aaacgccact ttcccttcag cgatcatttt ggcgatatgc tcgtctttcg ctttaatgcc
     2641 tggtttggca atggtgcaac ttttgttata gccaatcacc gcgatggcaa tcgacaatgc
     2701 cgcgcccgca ccggtattac acgcaccgat gtagtaatcc agttgcccag atttcacttt
     2761 cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa ctgccaggag ccgtcgcttc
     2821 aatggttttt ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt tcatgagtct
     2881 gtccttattg gaaaaattga gagggatttt cacgtaacat cacatccaca tcggcctgac
     2941 tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa taaatagtca tagccataac
     3001 caccattggc ttttaaatgg gagcggcgcg taatatccat cgacagcatg acgcggttca
     3061 gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg cttttcgtcc gggtagtaac
     3121 tgttcttacc aatggtgtcg aactgcacgt acgcgccgag atcgatcatc ttcaaaatgt
     3181 tgtcgagatt gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga tcaactccgt
     3241 gggcttgcaa cagagccagt tgttccagcc ccatggtgct gaacgaagta tgcgtggaga
     3301 tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat aaacaccttc tcttccagcg
     3361 gcgtaatctt cccttcgcta gagccgatct ccgcgataat cccggctttc aggtcggtgc
     3421 catcgatacc ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc acgctgcggg
     3481 tcgccacatg ttccgggaaa aacgcgtcct ggtaataacc ggtacaggcc accacgttga
     3541 tccccgtctc gcgcattaca tcaagcataa attgcgcatt gcgccccatg taacggttgg
     3601 tcatctcaat cacattacgc acgccccggg tcatcaggtc gttcatctcc tggcaaatga
     3661 acgcatactg atcaaggcgg cagtccacgt tgtttttaaa gccggagaga tcaatatgca
     3721 gatgctcatg ggcgagggtg taacccgtcg gatcaaaact catagggtgt ctcctgcaaa
     3781 ccggagggag gaaagaaaag agcgaccatt ttgtggcggt ggcgcgcgga aaaattcaca
     3841 cacggtagcc cccacatcag aaagcgtggt gcgcacaccg agctgcgtag cgatcatccc
     3901 ttgctgataa accagcactg gcaccacttc gcgggtatgg tggctgtgac caatggtcgg
     3961 atcgttaccg tgatccgcca tcacgaccag gcaatcatct ggctgcatcg cctcaacaag
     4021 ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca taacgtgcga cgtcttctgc
     4081 atgaccagcg aggtcggttt cctggatatt gatgcaaata aacgccgtcg gatgcgtgtt
     4141 aaattcgtcg agggtgatat ccataatccg ctggctatcc accagatttt gccagctcac
     4201 gccataagga ttgttgacga tatctgccac cttacccacc agcacggttg gcacgcctgc
     4261 ttcatacagt ttttgtggca cctgcacttt ttcatcgacg ccatagccca tatgcacgac
     4321 ctggaaaccg ttgtcataag cgccagaacg cggcgcattg ataccaataa agcgcccttc
     4381 tttgctttct gcggcatcga gaatgcgttg actgtcggtt aacaggccac caaatgtaat
     4441 gacccgaccg acctgtacct gctcacgcac gatacgacca attttgattg cgtcgtcaaa
     4501 agagatcaca gagagattgg cggtaatgtt atagacctgg cctaaatccg cctcgagatt
     4561 atcgccaatc gcaaccgcct gattgaccaa cagaaattgc agttcatcgc cacggcgctc
     4621 cacctgccaa ccggcggata ctaatgcctg ctcaacacgg tcaatcacat cgcaaaaagg
     4681 catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca taaaggtatc
     4741 gccaccttca tgttgcagct ctgccacgcc ccaggttgcg gaatctgacg gctgcatatc
     4801 gcctggcgca taacccaatg cgttgattag ccccagcttc tccagcgttg gtagctgcaa
     4861 atgcggcaac tggctcagga tgtgaccaca ggtattcgct cccgcatctt gcggacgcac
     4921 cagcgtgaca tctttcattg cccctacgcc aaagctatca atcactaaca ccacaaatcg
     4981 cgccattacg cccccagaga gtttccaaga ctgtcataac gaccaacgat tttcggttcg
     5041 ccacggtgaa taccggacac cagcaccaca tcgctacggg tgacaaaaat ctgcgtgcgg
     5101 aaacagagca ccactgcact gctcaccgga tactcgcctg ccagcggcag ggtgtagtcg
     5161 atactgctgt catccacagt tttgagattg gtttcagtaa tcttttgatt ttctggcgta
     5221 aacaccagcg catgttgcgc atgaccacga cggtagtaac cgccgccgta gcagtagctg
     5281 tcgccacgga aatgatggga gatttcactt aaccagagca tcgcgatacg ttcaggctga
     5341 tcgccctgct ggtttgccgg aatagtgccc gtcagcgcat gaccgggttc ggcatgagtc
     5401 acaccgtatt ccgccagcaa tggcagcgaa gtgcagctgg tcgctgaagg cgcgttcagt
     5461 tgctcaagtg caataccaga tttcgccagt tgatcccgtg cctgtatcag cgtgtgaaga
     5521 ttcggtgtcg gcaaaacttt tccggcagcc tcatcccaaa gcaggcaagg gaaatgggta
     5581 agtccggcta aatgcagccc cggcagtttc ttaatttcag caaccacctc gtgcagcgaa
     5641 tgctgtacaa aaccgctctc ctgccccgga taaagaaaat catcgtcgct ataaactttg
     5701 agcagcacaa actgcacacg tccggccttc accgctgccg cagaaacttc ccgcgctttg
     5761 tcgagagtaa acacggtgat gacgtcggtg ccctgttcaa cggcgtcagc aacctgatga
     5821 caagggattt gtaccagatg cccctgatgc gccaccgaca aaccagcgcg gcgcatgact
     5881 cgcgcctctt tgtaatccac cgccacaatg ccgctgtaac ctaatgccag caatttttcc
     5941 gccagccatg ggttacggcc aaactgcttg gtcatcagat acagttcaat gccgtaaagc
     6001 cgcgccgtct caatcagtcg cttaccgttt tccagtacct gatccacgtc gatcacccag
     6061 ctgtccgggg cgatcttgcc ctgctgccac aaacttagtg cggcggaaat cagcgccggg
     6121 ttctggcgtt tcaatgcttc tacaaacatc ttgttgcctc gtttctgttc cttgaattaa
     6181 atattcattt ttttgaatat ttaggttgtg cgacgtggat ttgtcgggag cttcaaacct
     6241 cttcgttagc agccatccat aatccataaa ggttggccag caaatagcct tcttcacacg
     6301 ggttaacttc cagtgcgaat tccttcaaca acacctgatg caattgcaca acggcttgcc
     6361 agtggctgga ttgcgccagc tctgccagca attcgttatc cagcggctct atttcttcac
     6421 cacggcgact gcgcatcagt gcactcgcca tatgtgtcat cgccatcgtt ccttgctcac
     6481 tgcgcaccgg cagatggcac tctgtttcca gtacgttgac gacctgcatc atgcctttgc
     6541 agatgtcctt atcaataacg cctgcctcgc aaagcaggtt gagtctggtt tccatatcag
     6601 atgcctttcg tttaataact cggttcctgt tccccactca ctacccgttg ttgatgggcg
     6661 agcagggcgt gtttatctac aacggcacgc agcagttgtt gcattgggta atcatcgact
     6721 cgcgtcagga ccacggcttc ggtggcctgt aaaaatcgcg gatcgtctgt gagcggcgtc
     6781 gcctctaatc ccagcatggt cagttcgttt tccgccacca cgttccagat caccgcatcg
     6841 acatcgcctt taacaatgcg ttgtaaactc tcgtgataag agagatcgac tcgttccaca
     6901 tcactaccgc caaaaaaaac atcggtcatg attttctgat ccgccgaacg gttatccagc
     6961 cccacgcgct tcacgtttgc ggactcgcct ttacggcaaa tcaactggtg ctcgccaacg
     7021 taggtgtgcg gccccaactc cagcgcgagg cataaccctt tttgcgtgag ataactttcc
     7081 gccgccagtc gggaaaccac cgccatgtca tacacgccgt taagtaaaca ctccacgcga
     7141 atatccgcgc cgcgcatatg cgcatagtaa aaaggaatgc catcaaactg agctttcagc
     7201 ccgctcgcta aaccttcgta caagcgggta tagggcaaag gcatcgcaca taccacgttg
     7261 ttgatatcca catgactcag caatgctttg ttatccatct cgaccagata actgccattg
     7321 cgcccacggc gttcaatccg tatcgctccg cttgattcca gcgttttcaa cgcggcctgc
     7381 gtcaggccaa ccgatgaacg gcattcagtt gccagctcat ctatggtttt caacctatta
     7441 ccgcattttt cacccaacaa ataacgggcc agcgtcgtga taacgacgcc ttcttttttg
     7501 ataaacgttc gacgcataat atattttcaa taaaatgaat gtttatatct tcattaaaat
     7561 gaagataagt ggcaactgtg agatgtggaa acggcgaggc gcttcacatt ttttcggatt
     7621 attgcaaatg aggataaaaa aaccgggttt ccccggtttc agagtgatga cgaagtgatt
     7681 tattcgacaa tgcgagcaaa gggtaattcc cactggcgct tgcctgctcc cctcacccta
     7741 accctctccc caaaggggcg aggggaccgt ccgagcgttt tgatattacg gcttcgccac
     7801 aaaaccaatc gcttcgtaca ccgctttcag cgtacgggaa gcgtgtacac tggctttttc
     7861 tgcgccgtct ttcatcacct gttgcaggaa ggcttcatcg ttgcggaaac ggtgatagcg
     7921 ttcctgcaat tcagtcagca taccggaaac ggcatcagcc acttcacctt tcagatgacc
     7981 atacatcttg ccttcgaact gtttttccag ttctgggatg ctctggcccg ttaccgcaga
     8041 aaggatatcc agcaggttgg aaacgcccgc tttgttctgc acatcgtagc gaactaccgg
     8101 cggctcgtcg gagtcagtga ccgcgcgttt gattttcttc actaccgatt tcggatcttc
     8161 cagcaggccg ataacgttat tgcggttatc gtcagacttg gacatcttct tggtcggctc
     8221 cagcagcgac attacgcgcg cgccagattt cggaataaac ggctccggca ccttaaagat
     8281 ctcgccatac agcgcgttga aacgctgggc gatatcgcga ctcagttcca ggtgctgttt
     8341 ctggtcttca cccaccggta ccagattagt ttgatacagc aggatgtccg cagccatcag
     8401 caccggatag tcaaacagac cagcgttgat gttctcggca taacgcgcag atttatcttt
     8461 aaactgggtc atgcggctca gttcgccgaa gtaggtatag cagttcagtg cccagcctaa
     8521 ctgcgcatgt tctggtacgt gggactgaac aaaaatggtg cttttctcag gatcgatacc
     8581 acaagccaga tacaaggcca gcgtatccag cgtcgcttta cgcagcttct gtgcatcctg
     8641 gcgcacggtg atcgcgtgtt ggtcaacgat acagtaaatg caatggtagt catcctgcat
     8701 gtttacccac tgacgcagcg cacccatgta gttaccaatg gtcaattcac ctgagggctg
     8761 tgcgccacta aaaacgatgg gcttagtcat ttttcgattc ctgattttcg ctatgcggaa
     8821 gccctaatgc gggcagaagg tcatttatag actgataaat tacatcaggc tggctgagat
     8881 cgatagcctc gccgtagtta tatccgtagg ttaagccaac ggatgggcaa cctgccgctt
     8941 ttgccgcctg aatatcattg cgtgagtcgc cgacaaacag catctgttgt ggggcaattc
     9001 ccatccgctc agccaccagt aacagcgggt ccggatgagg ttttttgttt tgcacatcat
     9061 cgccgccgat aaccacgcta aagtatttgg caatatctaa ggcttcgagc agcggcgcga
     9121 cgaacggcgt cggtttgttg gtgaccaggc ctagcggcag gcctttagcc tgcaacgcgc
     9181 ccaacgtatc ggcaacgtgc gggaacaaaa acgtcccctc ttcggcaacc tcgccatagt
     9241 agcgatcgaa cagtttacgc agaatacgta cctgttcttc tgccggaatg tcgtcatcaa
     9301 cgggcggttt acccattgtt ttacgctgag tcgcacgttc ctgacgcgcc caggtcaatg
     9361 cgcgctccat cagaacatct gcgccgttac caatccaggt aataacgcgt tcttcacctg
     9421 cgacgggcaa ctccagcgca tacagcgcca tatctaccgc agcagcaaga ccgggagcac
     9481 tgtcgaccag cgtaccatca agatcaaaag cgacgccgcg aatatcttca aacttattca
     9541 tgacttacct ttgccagttc actgcgcatt tcatcaatga cttttttgta gtctggctgg
     9601 tcgaagattg ccgaaccggc gacgaacata tccgcgcccg ccgcagcgat ttcgccaatg
     9661 ttgttcacct tcacgccacc gtctacttcc agacgaatgt caaagccaga ctcgtcgata
     9721 cgacgacgta cttcgcgcag tttatccagt gtttgaggaa tgaacgactg accgccgaaa
     9781 ccaggattga cggacatcag caggatcaca tccagcttat ccatcacgta atccagatag
     9841 ctcagcggtg tcgccgggtt aaataccagt cccgctttac agccattttc tttaatcagt
     9901 tgcagcgtgc ggtcaacatg ctcggaggct tctggatgaa aggtaatgat gctggcacca
     9961 gcggcagcga aatcaggcac aatgcgatcg acaggtttca ccatcaggtg tacgtcgata
    10021 ggggcggtaa tgccgtagtt acgcaaggat ttcagcacca ttggcccaat cgtcagattg
    10081 ggaacatagt ggttatccat gacgtcaaaa tgcacgacat cagcgccagc tgccagggct
    10141 tttgcggtat cttcacccag gcgggcaaaa tcagccgaca gaattgaggg ggcaatcaaa
    10201 tactgtttca tccgcttctc cttgagaatt attttttcgc gggtgaaacg actcctggtt
    10261 tgtacaaagc cagcagttcg tccacctttt tacgtgtgcc gccgttgctg cttatactgc
    10321 gtcgaacttt gacgacatgc aattttgcgc gctgatacca ctcacgcgtt aacatcgtat
    10381 cgtgattgga gatcagcact ggaatatggc gctcaaccag accttcggcg atctccgcca
    10441 gatgcgcttg ttgttcaagc gtaaaactgt ttgtgtgata cgccgtaaag ttggcggtcg
    10501 cagacagcgg tgcataaggc ggatcgcaat agacgacgga tgcatcatct gcgcgcgcca
    10561 tgctatcggc gtaagactca caatagaaaa aggcattctg cgctttttca gcgaagtgat
    10621 acaactctgc ttccgggaaa tagggttttt tgtagcggcc aaacggcacg ttaaactcac
    10681 cgcgcagatt gtaacgacac aggccgttgt aaccgtagcg gttcaaatat aaaaacagta
    10741 ccgcccgacg gaacggatcc tggcttttgt tgaactcttc gcggaactga tagtagacct
    10801 cggcgcaatt tgtttcggga acaaacagct cgcgtgcggc ctgtacgtac tcatcagtac
    10861 gcatcttcac aatgttatag agactgatca ggtcgctatt gatatcggca aggatataac
    10921 gagaaaagtc ggtgttgaga aacaccgacc cggcacctac aaaaggctca accagacatt
    10981 cgcccttggg caaatgccgt ttaatatcat caagcagggg atacttgccc cctgcccact
    11041 tcaaaaaagc gcgatttttc ttcatgctga ctaactaatt acaccttctc cggctgtgga
    11101 gaaagctccg acagcatcca gcgcttcagg caactcccgc aacgataacc cattgcggga
    11161 gataaccttg attacttcag atcggcctgt acctgacgca gcggtttcgc ccacgggttt
    11221 ttggcctgaa catctgctgg caatgtagat accgcttttt tcgcctcttc tttcgaagcg
    11281 tacacgccag aaaccaggac ataccacggc tgaccattac gcgtcgtttc atagacaacg
    11341 tagtttttca gattctcttt cttcgcccaa ccgttcaggt tgtcgtagtt agaggaactg
    11401 ctcagctgca gagtgtaatg gctggacggt gccgatttca acgaaccaac attacctgcg
    11461 gtcttccctc cagcggctgg tgttgccgtg gtttgcgccg gggatgccgt ctgtactgga
    11521 gccgtagtcg ccgtctcttt tggtgctggc gcagaagtcg cagccggtgc cttcgtgctc
    11581 gccacaggag cagctggttc agtacgcttc ggcgtctgtg ctaccggctt cggttccgtt
    11641 ttcacggttg cttgcggttt tttcggttca atcaccgcct gctgacgagc tggacgcgta
    11701 gtggccggac gttcagcagt ttgcgttttc gccgtgtcac gcgatgcatt gccattgcgt
    11761 actggcgcga cagttgctgg ttcagtcggc aatgtagaat tgaccgccac attgttcaac
    11821 tgttgctgat tttgtggctg ggtcagcgca ttgttcaggt caccctgcac ttcaacacgt
    11881 tgttgaccat ccgttgccgc cggggtttgc ccttgagtcg gcgtagaaga gatcggtggc
    11941 agagaaacat cctgctgagt attttccgca gacgtggttc ccggcgctgg ctgcacccca
    12001 ttcgcctgat cggtcgcatt gcctgcaaga tcaatactct tctcgccaga cgcggtttga
    12061 tcgctggaag aggtcgaggg ggcttttagc gcagaaccga taccgatgat caacagcagt
    12121 agaaccagaa tgccgacgcc catcatcata tactgacgag aagcgggttt actggctgct
    12181 tttttgcgct tacgcggacg acgctctacg cgctcttcat ccacggtttc atcttcggat
    12241 tcgtcaattt cttcttcgat ttccggttcc tcattgcgct ctttttgcgc acgagtcgga
    12301 cggcgatcgt cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg ttcgccacgt
    12361 tcagtacgct cagaacgttc agaagattga cgagaacgac cagtacgacg atcgctggga
    12421 tcgggtttca gctcgtcttc tggtttgaat tcatccattt aacaccccac taaaaggtta
    12481 atgcttacca cgttgcaatt aacctgaagc taatagaccg cttgataagc ggcctgacct
    12541 ttcttgttgt tacgctgatt gacaatcggc aatggcgtta agaacaagct cgtgcgaaac
    12601 gccgctgcga acttcactct taccaattgc caacggaaga attaagcgta tctctcccgc
    12661 aaggactttc ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca tttcgcgcgg
    12721 cccattgacc ggtaacccag cccgcttgag cagggttata atacgctgcg tttcggcaga
    12781 actaaactgc ccgagacgtt ccgacgtccg cgccgccatc accatacccg cagcgaccgc
    12841 ttcaccatgt aaccaattgc cataccccat ttcagcttca atggcatgac caaaggtgtg
    12901 tcccagattc agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga caacttctgc
    12961 cttcagttca caacaacggc gaatacagta cgccattgcc ggaccgtcca gacgcaacaa
    13021 cgcatccaga ttctcttcca gccagttaaa aaacgcaccg tcaagaataa tgccgtattt
    13081 gatgacttct gccagccccg acgctaactc acgcggggga agcgttttca gacagtcgag
    13141 atccaccacc actgaagcag gctggtagaa cgcgccaatc atgtttttac cgaggggatg
    13201 gttgaccgca gttttgccgc caacggagga atcgacctgc gacagtaacg tcgtcgggac
    13261 ttgaatgaaa cgaacaccgc gctgataact cgccgccgcg aagccggtca gatcgcccac
    13321 tacgccgccg ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt gtaacaacgc
    13381 cgtaaagacg gtatcgagta cagccaggct tttatactgc tcgccgtcag ggaggataac
    13441 gctatcgacg ttaacacccg cctgttcaag tacgccgcgg accttatcga gatacagagg
    13501 agccagggtt tcgttggtga ccaacatgac ctgctcgccc gatttcagcg gtaagaatga
    13561 agctggttca ttaaacaaac cagatgcgat ggtaattggg taactacgtt ccccgagagt
    13621 aacgacaatc ctctccataa cgcgacatcc accttaatta ctgtacccgc agacgagtgt
    13681 atataaagcc agaattagtt gctttccagc atgtgaataa tctggtttgc aaccacttta
    13741 gcgctttgat catcagtacg aatggtcacg tcggcaatct cttcatacag cggattgcgt
    13801 tcattggcca acgcttccag aacttcacgc ggcggtgttt caacgtgcag caacgggcgt
    13861 tttttatcac gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag ataaacgaca
    13921 acgccacgag cggaaagacg gttacgcgtt tcacgggatt tcacagagcc gccgccagta
    13981 gccagcacaa taccctgttt ctcggtcaac tcattgatga ccttttcttc gcgatcgcgg
    14041 aagccttctt cgccttctaa atcgaaaacc cagcccacat cagctccggt tcgtttctca
    14101 atctcttgat cggaatcgta aaattccata ttgagttgtt gagctaactg gcgcccaata
    14161 gtgctttttc cggcacccat aggcccaacc agaaagatat tgcgtttctc tgccattttt
    14221 tcggtactac taagactatt cgttaatgat aaacccgctt cgctcagaga gcgccgcagg
    14281 acatgaactg aaacctcata agatattgcg agagtcagac tgaaaattat ctcaatactc
    14341 cagcgggttt ggcaactgaa taaatcaccc atccctctgc atatctggtc gctgcaagcg
    14401 cgctgccttg ctaccaccgc tctggcgata aatcaccggg taagattagc gtaaaaaaga
    14461 cagcaaaatg ccgcctgaat gataaatcat catcatggaa cgcaggcagc gaagagcacc
    14521 aactctcaaa tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat agatgaaaca
    14581 cgttcagcat aaaaacatta ccgcttttac ggctgtttac tcactggaaa ccagtcgtgg
    14641 cgtgataaac accactaact cgcgtcgttc atcttctttt ccgtcatgac gaaataattg
    14701 cccgaaccag ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt tatttttacg
    14761 ggtaaaaatg ccgcccagcg ccaacgtttc tccgcttttg acctcgacct gcgtttcgat
    14821 ctcctgctta tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct gccccggaac
    14881 gttctggctg atgtgtaatt tcagccggat gcgacctttt tgtaacaccg tgggcgtgac
    14941 ctccatcccc aggacggcct ctttaaattc caccgacgtc gcgccacttt ccccgctgga
    15001 aacctgatat ggaatttcgc tcccctgttt aatgctggca ggctgaagat gtgaggccag
    15061 cagacgcgga ctggcgataa tatccagctg ctgtttttgt tcgagcgcgg aaagctcaag
    15121 atccagcaag cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg tcgccgtcgc
    15181 tacggagagg tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt gttgcgcatc
    15241 ggccagcgtc catttcacgc ctaactcacg caaacttttt tcattaatgg tgacaatatg
    15301 cgccgacagc tcaacctgcc cgaccggcag atccatttgc gctacccact gttcaagcgc
    15361 gcttaacgcc gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat cgacggtcat
    15421 actccctttg gcactcagta gcttctcccc cgctttcgcc agttctcccg cgtcggcgta
    15481 ttgcagggtt atactgcgat tttccagcgg cagatttgcc tgcgcccgcg cctgctccgc
    15541 ctcctggcgg gcgatattgt tattctgcca ggcaatggaa tgcactgaga gaatgttgtc
    15601 ttcctgccgc gttatcagtc cggcgctttt cactacagtt tgtagtgcct gcttccaggg
    15661 aacatctgtt agatgtaacg acaccgtacc gctgacgtct ggcgacacga ccaggttcaa
    15721 cttctcctgt tcagccagcg cctgcaacac ctgagctacc ggaacgtcat ccaccatcag
    15781 cgtcactttt tgcggctttg ccgcctgtac gccgggtatc agcatcaaca gtagtgcggc
    15841 tatccattgc ttcatttgta tctccttgcc gttgccacaa ccattgtggc ggttcgcagt
    15901 ttgtcccggt acccagcgtt agtacgtctg gcgtcagctg taaaattgtc cagccgtttt
    15961 ccagcacatc gttttgctgc acccgtcgcc atttcttttg cccgtctttt attacaccga
    16021 tgatgcgctc gcctcgccct accatcccct gatagcgcca ctggctaagt tcgctaatcc
    16081 ggcatagatc ttccggcggt ttaaaagggt cacgcatacc ggttaaaagg cacaatgcaa
    16141 tacctgccaa caaccagcgt ttaaccctca tgcggcgtct ccagttgtag cgtgaacaaa
    16201 agatcatcac cttccacgct taacgaaaaa cggctcacgc tgacgttgcg ctctgccagc
    16261 cgtgtaaatg ccgacggcac tgcttcccac agcgttttca acgccaactc gcctccctgc
    16321 gcggatggat gccaggaaac cagttgcgcg ccggataact gaaaatccag tggcgaaaag
    16381 ggcagcgttt tttcctcgct gaagggagtg gtgtctacca ggcgatacag tgcggcccac
    16441 tgctggtgat gacttgcccg tagtcgaatt aatgcgtcgc gctcttcatg gtgtgtcgat
    16501 gatagaaaaa tgagcgtaac taacatcagc aaccagactg cccagcaaag ctggcggagg
    16561 cggggtgatg tggcgaacca ccagtcaaag aacatgttca tcgctaacct tccttgttaa
    16621 ctgatactca aattgccagc gtccctgcgc atcctgctgc gtggctcccc gctgattgag
    16681 atgaaaagac gcatcctggc ggagtgacgt ttctagtgcg tttaacgcgg taatgcttgt
    16741 tgtcagcccc ttgatctcca gcgttccctg ctgccagctt atcgttgtca gccaggcgtg
    16801 ctcgggtaaa agcgccgcca gtgcttccag cgcagattgc cagtcgcggg taaattgtcg
    16861 ctggcgctgc cgttgcgaac gctgttcgcg taactgctgt tgctccagca aacgtggctt
    16921 cgttatctgt aagctgcggg cgagttgttg ttccgcctga agcaaaactg cgtctatgcg
    16981 cgcttcggcg ctgcctgtca gacgcagtat tagcgttatc ccgacggcca gcagcagagg
    17041 cgcaacgaac atcagcaacc agaaacgcag aaaagcggtc cggcgttgct gtcgccaggg
    17101 caaaaaatta attggcgggt tcatcagtac gccttcccaa gcgccagccc cagcgcgatg
    17161 gcaaagtctc cacctgacgg cggtagcggc ggctgacgaa cagaaacggc ctcccaggga
    17221 tcaaatcccc cttcgccaca tatcgcgacg ctttcgggat caacggataa cgctgccgcc
    17281 agctccttcg cgctagtcat ccccaccgcc agtttgcgcc cccagcgata gcgtgtcgcc
    17341 cacagccact gttcgttatc acgccaggcc agacattgct gatgagaagg taaaaaaggc
    17401 aggaatcgct gtaatgcact ggcatccggg gtgatcgcac taacatgaac acgcaacctt
    17461 tctgccagcg taagcagcgt tgccagctct ttgctttgcg cggcagtcac gttataagcg
    17521 gggctgagtg agtcttcgct ataatcgaag cgcagggagt ccggatccat atccagctcg
    17581 cgggccatcg tccctgacag ccaggccgtt tgctcccgct caccaaggga catcgacggg
    17641 cgcggaaatg accgctgtaa tgtgcgactg gcgggaaacg ccaacataat gtgatgacgc
    17701 tgcggcagtt cgcgactcca aggtaacaac gttttagcca gctgctgcgc atcaacaatc
    17761 cgcccatctt tgataatgtc gttctccagc ggcaaccgcc accagcgttg caaaaagcat
    17821 tcttttgcgc cccgtacgat cgcaaccgct accgcttctt gctgttgtaa atgcaaacca
    17881 atttgccaga tcttaaatgc cattgtgatg atctccttat cacccgtcac tctgacgggt
    17941 atatcaatgc gtctggcttg cctttatact accgcgcgtt tgtttataaa ctgcccaaat
    18001 gaaactaaat gggaaatttc cagtgaagtt cgtaaagtat tttttgatcc ttgcagtctg
    18061 ttgcattctg ctgggagcag gctcgattta tggcctatac cgctacatcg agccacaact
    18121 gccggatgtg gcgacattaa aagatgttcg cctgcaaatt ccgatgcaga tttacagcgc
    18181 cgatggcgag ctgattgctc aatacggtga gaaacgtcgt attccggtta cgttggatca
    18241 aatcccaccg gagatggtga aagcctttat cgcgacggaa gacagccgct tctacgagca
    18301 tcacggcgtt gacccggtgg ggatcttccg tgcggcaagt gtggcgctgt tctccggcca
    18361 cgcgtcacaa ggggcgagta ccattaccca gcagctggcg agaaatttct tcctcagtcc
    18421 agaacgcacg ctgatgcgta agattaagga agtcttcctc gcgattcgca ttgaacagtt
    18481 gctgacgaaa gacgagagcc tcgagcttta tctgaacaag atttacctcg gttaccgcgc
    18541 ctatggtgtc ggtgctgcgg cacaagtcta tttcggtaaa acggtcgacc aactgacgct
    18601 gaacgaaatg gctgtgatcg ccgggctgcc gaaagcgcct tccaccttta atccgctcta
    18661 ctcaatggat cgtgccgtcg cgcggcgtaa cgtcgtactg tcgcggatgc tggatgaagg
    18721 gtatatcacc caacaacagt tcgatcagac acgcactgag gcgattaacg ctaactatca
    18781 cgcgccggag attgctttct ctgcgccgta cctgagcgaa atggtgcgcc aggagatgta
    18841 taaccgttat ggcgaaagtg cctatgaaga cggttatcgc atttacacca ccatcacccg
    18901 caaagtgcag caggccgcac agcaggcggt acgtaataac gtgctggact acgacatgcg
    18961 ccacggctat cgcggcccgg caaatgtgct gtggaaagtg ggcgagtcgg cgtgggataa
    19021 caacaagatt accgatacgc tgaaggcgct gccaacctat ggtccgctgc tgcctgccgc
    19081 agtcaccagc gccaatcctc agcaagcgac ggcgatgctg gcggacgggt cgaccgtcgc
    19141 attgagtatg gaaggcgttc gctgggcgcg tccttaccgt tcggatactc agcaaggacc
    19201 gacgccgcgt aaagtgaccg atgttctgca aacgggtcag caaatctggg ttcgtcaggt
    19261 tggcgatgca tggtggctgg cacaagtgcc ggaagtgaac tcggcgctgg tgtcgatcaa
    19321 tccgcaaaac ggtgccgtta tggcgctggt cggtggcttt gatttcaatc agagcaagtt
    19381 taaccgcgcc acccaggcac tgcgtcaggt gggttccaac atcaaaccgt tcctctacac
    19441 cgcggcgatg gataaaggtc tgacgctggc aagtatgttg aacgatgtgc caatttctcg
    19501 ctgggatgca ggtgccggtt ctgactggca gccgaagaac tcaccaccgc agtatgctgg
    19561 tccaattcgc ttacgtcagg ggctgggtca gtcgaaaaac gtggtgatgg tacgcgcaat
    19621 gcgggcgatg ggcgtcgact acgctgcaga atatctgcaa cgcttcggct tcccggcgca
    19681 aaacattgtc cacaccgaat cgctggcgct gggttcagcg tccttcaccc caatgcaggt
    19741 ggcgcgcggc tacgcggtca tggcgaacgg tggcttcctg gtggacccgt ggtttatcag
    19801 caaaattgaa aacgatcagg gcggcgtgat tttcgaagcg aaaccgaaag tagcctgccc
    19861 ggaatgcgat attccggtga tttacggtga tacgcagaaa tcgaacgtgc tggaaaataa
    19921 cgatgttgaa gatgtcgcta tctcccgcga gcagcagaat gtttctgtac caatgccgca
    19981 gctggagcag gcaaatcagg cgttagtggc gaagactggc gcgcaggagt acgcaccgca
    20041 cgtcatcaac actccgctgg cattcctgat taagagtgct ttgaacacca atatctttgg
    20101 tgaaccaggc tggcagggta ccggctggcg tgcaggtcgt gatttgcagc gtcgcgatat
    20161 cggcgggaaa accggtacca ctaacagttc gaaagatgcg tggttctcgg gttacggtcc
    20221 gggcgttgtg acctcggtct ggattggctt tgatgatcac cgtcgtaatc tcggtcatac
    20281 aacggcttcc ggagcgatta aagatcagat ctcaggttac gaaggcggtg ccaagagtgc
    20341 ccagcctgca tgggacgctt atatgaaagc cgttcttgaa ggtgtgccgg agcagccgct
    20401 gacgccgcct ccgggtattg tgacggtgaa tatcgatcgc agcaccgggc agttagctaa
    20461 tggtggcaac agccgcgaag agtatttcat cgaaggtacg cagccgacac aacaggcggt
    20521 gcacgaggtg ggaacgacca ttatcgataa tggtgaggca caggaattgt tctgattaaa
    20581 aaggcgcttc ggcgcctttt cagttggctg acaaagtgca tttgtttatg ccggatgcgg
    20641 gtgaacgcgt tatccggcta acaaaatcgt gaaaaatcaa tatattgcag gaaacccata
    20701 agcctgataa gcgttgtgca tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt
    20761 caaccattcg cgcacgagga acagcgcgct gacattacgc gcttcgttga agtcagggtc
    20821 ttccagcaaa tccatcatat gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc
    20881 atcgccttcc agcgattccg ggtagagatc ttgcgcgacc acgatattca ttttgctgga
    20941 aaagtaagac ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg caccaaatcc
    21001 aacctcttct tttagctcgc ggttagcggc ttcgtagacg ctttcacccg gatcaattaa
    21061 tcctttcgaa aaacctaatt cgtaggattc agttcccact gcgtattcgc ggatcaggat
    21121 caggtgatcg tccacaatcg gcacaatcat cactgcttcc cggttggttg gacgcattcg
    21181 ttcataaaca cgccgcacgc cattgctgaa ctccagatcc acgctctcga cggtaaacag
    21241 tcgggaacgg gctacagttt caacattcag aatggtgggt ttttgtaatg atttgctcat
    21301 cgtgggatct atgctgtgaa atcagcggtt attgtgcgat atcggacacg ctttcggcaa
    21361 tgtgaattgc atgttattta catttatgta acttaataaa taatcgtcct caaatcaaat
    21421 taaaagtcaa taggttgaaa taactccagg aatttgctga tattccgcct tcagagggtt
    21481 tgctatgatc agcggttact gtgatgtgct taatgatgct caagttaaac tccacgcttg
    21541 ccgatagcca accgcagaat catgtattgt gtctggtgcg actgaccacg cctgacagac
    21601 taagtaagat ggggaaagca tgagcaccat tgtgattttt ttagctgctt tgctggcctg
    21661 ctcactactt gcgggatggc tgataaaagt gcgatccaga cggcgtcagc tgccctggac
    21721 caacgccttc gcggatgcgc aaacgcgtaa actcacacct gaagaacgta gcgccgttga
    21781 aaattatctt gagagcctga cgcaggtatt acaggtgcct ggcccaacgg gagctagcgc
    21841 ggcaccgatc tctctggcgc tgaatgccga aagcaacaac gtcatgatgc tgacacacgc
    21901 tatcacgcgt tacggcatct ctaccgacga tccgaataaa tggcgttact acctcgattc
    21961 ggtagaagtc cacctgcccc ctttctggga acaatacatc aacgatgaga ataccgttga
    22021 actgatttat accgattcgc tgccgctggt tatttcactc aacggtcata cgctgcagga
    22081 gtacatgcag gaaacgcgcg gctatgcctt gcaacctgtt ccgtccacgc aggcgtcgat
    22141 tcgcggggaa gagagtgagc aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga
    22201 atatgcgctg agtcgtccgc gcgggctgcg tgaagcgttg ctgatcgtcg cctccttcct
    22261 gatgttcttt ttctgcctga ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc
    22321 attactgctg ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa aatcctccct
    22381 gcgagaaatt cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga
    22441 tcaggaacag atcaacaata tttcactcgg tattatcgac ctggtctatc ccgcacactg
    22501 gcagccatac attgctcagg atctcggtca acaaaccgat attgatatct atctcgaccg
    22561 ccacgtagtg cgtcaggggc gatatctttc gctgcatgat gaagtaaaaa actttccgtt
    22621 acagcactgg ctgcgcagta cgattatcgc tgcgggctcg ctgctggtgc tgtttatgct
    22681 gttattctgg atcccgctgg atatgccgct gaaattcact ctctcatgga tgaaaggcgc
    22741 gcagaccatt gaagccacca gcgtaaaaca actggctgac gctggcgtgc gggtaggcga
    22801 tacattgcgt attagcggta cgggaatgtg taatattcgc acttccggaa cctggagcgc
    22861 gaaaaccaat tcaccttttt taccgtttga ctgctcgcag atcatctgga atgacgcccg
    22921 ctcattacca ttaccagaat ctgaactggt caacaaagcg acggcattga ctgaagcagt
    22981 taatcgccag ctgcacccta aaccggaaga tgaatctcgc gtcagtgcct cattacgttc
    23041 agcgattcaa aaatccggca tggtatttct tgatgatttt ggcgacattg tactgaagac
    23101 agcggattta tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct
    23161 cggcaacagt aaagactggg acgcgctggt aaaacgcgcc aacgccggga agctcgatgg
    23221 cgtgaatgtg ttattacgcc cggtgagtgc ggaatcgctg gataacctgg tggcaacctc
    23281 caccgcgccg tttatcacgc atgaaacggc gcgagcggca caatcactaa acagtccggc
    23341 ccccggcgga ttcctgattg tcagcgacga aggcagcgat tttgttgatc agccctggcc
    23401 ttcggcatca ctttacgact acccgccgca agaacagtgg aacgctttcc agaaactggc
    23461 acaaatgctg atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga
    23521 cgccaatggt acgcagcata ttggccttca tccgatcccg gatcgttccg gcctgtggcg
    23581 ctatctcagc accacattgc tgctactgac gatgctgggt agcgccattt acaatggcgt
    23641 acaggcctgg cgtcgttacc agcgtcatcg cactcgcatg atggagattc aggcctatta
    23701 tgaaagctgc ctgaacccgc aactgatcac cccttcagaa agccttatcg aataacacgt
    23761 ttgcgcggca ggttatgcta ccctgtcgcg caaattgctt cactctggag atttccctca
    23821 tgcatatcaa cattgcctgg caggacgtag ataccgttct gctggatatg gacggcacat
    23881 tgctcgacct cgccttcgat aactatttct ggcaaaagct ggtgcctgaa acatggggcg
    23941 cgaaaaacgg ggttacgcca caggaagcga tggaatatat gcgccagcaa tatcacgacg
    24001 tacagcatac gctaaactgg tactgtcttg attactggag tgagcaactg ggtctggata
    24061 tctgtgcgat gaccaccgag atgggaccgc gtgccgtact gcgtgaagat accattccgt
    24121 ttcttgaggc actgaaagcc agcggtaagc agcgaatttt gctcaccaat gcgcatccgc
    24181 acaacctggc ggtaaaactt gagcataccg gtctggacgc acaccttgat ttattacttt
    24241 ccacccacac atttggttat ccgaaagagg atcagcggtt atggcatgcg gtggctgaag
    24301 ctacgggtct gaaagctgaa agaacgctgt ttattgatga cagcgaagcg attctcgatg
    24361 ctgccgcgca atttggtatt cgttactgcc tcggcgtgac taatcctgat tccgggattg
    24421 ccgagaaaca gtatcaacgc catccgtcac tgaatgacta ccgccgcctg atcccctcgc
    24481 taatgtgaag gagacgccat gaaagaaaaa cctgctgttg aggttcgact ggataaatgg
    24541 ctatgggctg cccgttttta taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt
    24601 aaggtgcatt acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg
    24661 ctcactctgc gccagggaaa tgacgaacgc acggtgattg taaaggcgat tactgaacag
    24721 cgtcgccccg ccagcgaggc agccttgctg tatgaagaga ctgcggaaag tgtagagaaa
    24781 cgcgaaaaaa tggcgctggc acgtaaactt aatgccttaa ccatgccgca cccggaccga
    24841 cgcccggaca aaaaagagcg ccgcgacctg ttacgattta aacacggcga cagtgaataa
    24901 ctgtcacctg caagagagat gattatgccg caacatgacc aattacatcg ctatctgttt
    24961 gaaaactttg ccgtgcgcgg cgaactggta accgtttcgg aaaccctgca acagatcctt
    25021 gagaatcacg attatccgca gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc
    25081 agcctgttaa ccgctacgct gaagtttgat ggtgatatca ccgtacagct gcaaggcgac
    25141 ggtccgatga atctggcggt tattaacggt aacaataacc agcaaatgcg cggtgtggcg
    25201 cgcgtgcagg gcgaaattcc agaaaatgcc gacctgaaaa cgctggtcgg caatggttac
    25261 gtggtgatca ccattacccc gagcgaaggc gaacgctatc agggcgtggt tggtctggaa
    25321 ggtgataccc tggcggcctg cctggaagat tactttatgc gttctgaaca gctgccgacg
    25381 cgcctgttta ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg
    25441 caggtaatgc ctgcgcaaaa tgcccagcag gacgactttg accacctggc gacgctaacc
    25501 gaaaccatca aaaccgaaga actgctgacc ttaccggcaa acgaagtgtt gtggcgtttg
    25561 tatcacgaag aagaggtgac ggtttacgat ccgcaggatg tggagttcaa atgcacctgc
    25621 tcgcgtgaac gttgcgccga tgcgctgaaa acgctgcctg atgaagaagt tgatagcatc
    25681 ctggcggaag atggcgaaat tgacatgcat tgtgattact gcggtaacca ctatctgttc
    25741 aatgcgatgg atattgctga aatccgcaac aacgcgtctc cggcagatcc gcaagttcat
    25801 taatgagtat gtccggcaga gagggctctg ccggatttta cgacgtaatg gcctgatgtg
    25861 ctgcgcttac caggcctgtg aaataaatcg caaattattg ttggtcagat acagcgtcgt
    25921 atccgttaaa agagttgtca cttacttaag tttatttcca gcgtttcacc atctaccgtc
    25981 agatcgcgcc acgctttcgg cctcgtcccc agttttccat ccttcgcagc tgtgagtaat
    26041 ttttctttcg ttatttcgtt cggaagtaaa ctcatatcaa gcgcgtccca ttcttttctg
    26101 tctgagtcaa agccatagac aatgactctg tcatcattaa aagcaaacag gatccgctcc
    26161 gcctgaccat cactattcag atcttgctcg accaggacgc aggcgtcttt ttcaatacag
    26221 gtcatcacgt tatagcgatc ctggattaag gccgaccaga atgtcgcatc cggtttaaca
    26281 gaaccagggg caattaacac attttcggct aatacttttt ccgatacctg ttcctgaaga
    26341 tgttgctctc catctaacgc catcagcaga tcccgcgcgc gtttcgggtc tttcataaac
    26401 ccggcatcgc ttttcagcga ctcaagcgcc gcacgtccat agcgaccgct ctgttcgagc
    26461 atgtagatag ttacctggtc tggcgtgttt ttgccgctct gataacgcgc catatgactg
    26521 ttcacgctaa tacgcatact gtccagcacc ggcgaattaa gaagcaccag tatcaccaac
    26581 accaataacg aaaccgcaag gttcactttg ccctgaagaa caaggggatt ttgcccatta
    26641 cgccagacga tactgacaaa ataccccagc gaccacacca gtaacaccag caccgccagc
    26701 gcgccctgca agcggtcgac agtccagccg tactgagcga cccgcagcca taacgcccag
    26761 gcggcgacga acacatacag cggagcaacc agcaaagcgg ttttaatcag gcaacgcaac
    26821 ggccccgtcc agggaagtga cgctttttgc ggatcgcgga caatagccat taagatcaat
    26881 tgcaaaaagg ccagcgttaa cagcaacccg gcggcggaga tgtggcgaga aatcgcgctc
    26941 aggcccgtaa acggcagagt gatgataaac atcagggtta gcaatgatac taacggcagc
    27001 aaccccgtgg cgattaacgt gaacaacttt tgaatagagt cgattaaacg tgactgtgtg
    27061 cgcgcgagaa tcaccgccag tgcggtaacc agacctaatg taagatacat aaaccagtcg
    27121 gttgcaaaaa agagcgtatt gaaaaacttg atgccaacaa gtttaaacaa ctcaccccac
    27181 agtaaaagca ccagccacgt caggccattg gcgagaaaaa tcaccagtaa tataagtacg
    27241 ttatgccata ctgactggta aaagtagcta tagcggctac tgccattgca gatacgaagg
    27301 ctttgttgta tccacggtag caacaacatt gccatcaaca gcagatagca gccaaaatcc
    27361 caaatcgcct tttcagctct ccaggggttc atgccatcag tttgccactt cagccaaccg
    27421 ctcatccctg tcgtggcaat aaacaccagc gccagccatc cccaaaggcg cttctgttta
    27481 aaagagatca cggagaagag taaaacggat gaaaacgcaa cggtcgcagg cacgccataa
    27541 acaatccaac tgtgattttt tccggcaagc caggcgatca acaggtagca aaccagcccc
    27601 tgaagtaatc cggtagcaat catcccccag cgtgttgccg gtgaaagttc aacgttatcc
    27661 atgttggtta tccagaatca aaaggtgggt taattatcgc atccgggcag tagtattttg
    27721 cttttttcag aaaataatca aaaaaagtta gcgtggtgaa tcgatacttt accgattgaa
    27781 tttgcatcaa tttcattaag gaatgcgatt ccactcacaa tattcccgcc atataaacca
    27841 agatttaacc ttttgagaac attttccaca cctaaaatgc tatttctgcg ataatagcaa
    27901 ccgtttcgtg acaggaatca cggagttttt tgtcaaatat gaatttctcc agatacgtaa
    27961 atctatgagc cttgtcgcgg ttaacacccc caaaaagact ttactattca ggcaatacat
    28021 attggctaag gagcagtgaa atgcgcgtta acaatggttt gaccccgcaa gaactcgagg
    28081 cttatggtat cagtgacgta catgatatcg tttacaaccc aagctacgac ctgctgtatc
    28141 aggaagagct cgatccgagc ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg
    28201 ccgttgccgt cgataccggg atcttcaccg gtcgttcacc aaaagataag tatatcgtcc
    28261 gtgacgatac cactcgcgat actttctggt gggcagacaa aggcaaaggt aagaacgaca
    28321 acaaacctct ctctccggaa acctggcagc atctgaaagg cctggtgacc aggcagcttt
    28381 ccggcaaacg tctgttcgtt gtcgacgctt tctgtggtgc gaacccggat actcgtcttt
    28441 ccgtccgttt catcaccgaa gtggcctggc aggcgcattt tgtcaaaaac atgtttattc
    28501 gcccgagcga tgaagagctg gcaggtttca aaccagactt tatcgttatg aacggcgcga
    28561 agtgcactaa cccgcagtgg aaagaacagg gtctgaactc cgaaaacttc gtggcgttta
    28621 acctgaccga gcgcatgcag ctgattggcg gcacctggta cggcggcgaa atgaagaaag
    28681 ggatgttctc gatgatgaac tacctgctgc cgctgaaagg tatcgcttct atgcactgct
    28741 ccgccaacgt tggtgagaaa ggcgatgttg cggtgttctt cggcctttcc ggcaccggta
    28801 aaaccaccct ttccaccgac ccgaaacgtc gcctgattgg tgatgacgag cacggctggg
    28861 acgatgacgg cgtgtttaac ttcgaaggcg gctgctacgc aaaaactatc aagctgtcga
    28921 aagaagcgga acctgaaatc tacaacgcta tccgtcgtga tgcgttgctg gaaaacgtca
    28981 ccgtgcgtga agatggcact atcgactttg atgatggttc aaaaaccgag aacacccgcg
    29041 tttcttatcc gatctatcac atcgataaca ttgttaagcc ggtttccaaa gcgggccacg
    29101 cgactaaggt tatcttcctg actgctgatg ctttcggcgt gttgccgccg gtttctcgcc
    29161 tgactgccga tcaaacccag tatcacttcc tctctggctt caccgccaaa ctggccggta
    29221 ctgagcgtgg catcaccgaa ccgacgccaa ccttctccgc ttgcttcggc gcggcattcc
    29281 tgtcgctgca cccgactcag tacgcagaag tgctggtaaa acgtatgcaa gcggcaggtg
    29341 cgcaggcgta tctggttaac actggctgga acggcactgg caaacgtatc tcgattaaag
    29401 atacccgcgc tattatcgac gccatcctca acggttcact ggataacgca gaaactttca
    29461 ctctgccgat gtttaacctg gcgatcccaa ccgaactgcc gggcgtagac actaagatcc
    29521 ttgatccgcg taatacctac gcttccccgg aacagtggca ggaaaaagcc gaaaccctgg
    29581 cgaaactgtt tatcgacaac tttgataaat acaccgacac ccctgcgggt gccgcgctgg
    29641 tagcggctgg tccgaagctg tgatgatttg aagctggata atatctatcc agtatcttag
    29701 agaaagcaaa acgggagaca ccttcgcctc ccgttttatt tacccttctt ttgtcgtgcc
    29761 ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct
    29821 ggtgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa tcgccagccc
    29881 taatcccgtg ccgctaatgg tgcgcgcact gtcgccgcga acaaacggct ggaaaaggtg
    29941 cttacgctgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc aagcgcgatt
    30001 cggctccgtc ccgctgctga ctttgatcca gccattgccg taacgggcgg cattgaccac
    30061 catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg
    30121 gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaataa cctcaccgag
    30181 tactgcgttg agatccgcca tttccatcgg catctcctgc ccggtgcgca ggtaatcgat
    30241 aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt cggccaggta
    30301 gccatcctgt tcgctcatca tctcggtcgc caggcgaata cgcgtcagcg gcgtgcgtaa
    30361 gtcatgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct taacaccagc
    30421 cgccatatgg ttaaaggcgc gggtaaccga acgcacctcc gaagcgccat actcacgcag
    30481 cggcggcgga ataatccctt taccgacctg caaagcagca tgttcgagat cgaccaacgg
    30541 tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca taatcgccaa
    30601 cgtatagcgg aacagcgggg aaaaatcgcc ctgatgaatt tcggtcagcg gcacccgtac
    30661 ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt tgttgacctc
    30721 aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc tgatggctta agaattcata
    30781 gtgttgtgcc caacgcagac ctgcctcttc ggcagcttcg ttggagtaga gagagatccc
    30841 cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg tgccgtcctc
    30901 cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt tattaaactg
    30961 ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca ccaggctggc
    31021 gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg
    31081 caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccaggcc ccaaacggtc
    31141 tgaatgtaac gcggatgcgc cggatcttct tccaccatgc ggcgcagacg tgaaatctgc
    31201 acgtcgatgg agcgttccat tgcggaatat tcacggccac gggcaaggtt catcagctta
    31261 tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc aaactcaccg
    31321 ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg
    31381 aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggtag ctcgttcgcc
    31441 tgacgacgca gcaccgcacg gatacgggcc agcagttcac gcgggttaaa cggttttgga
    31501 atgtaatcgt cagcgccaat ctccaggcca acgatacggt ccacttcttc ccctttcgcc
    31561 gttaccataa tgatcggcat cgggttgctc tggctacgaa gacgtcggca aatcgacaag
    31621 ccatcttcac caggtaacat caaatccagt accataagat ggaaagattc acgagtcagc
    31681 aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg ttcggtgaga
    31741 taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat cttgtagttc
    31801 tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta ttcttaaaaa
    31861 agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg ttacaaagca
    31921 tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat tctgttgata
    31981 tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca acgagatgaa
    32041 aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta attatctctg
    32101 gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg
    32161 cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt
    32221 gcgctatctc actaaatgca tggaaaatct caaaatcgtc gattactccc ctcagcagga
    32281 aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg tgactcaccg
    32341 tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct ctatcgattc
    32401 cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc
    32461 tgccggggaa gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt aaggaagagt
    32521 cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact atcccctgat
    32581 ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc gcattattgc
    32641 gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg
    32701 gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc tggatgacac
    32761 gcaactgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag agagacgtca
    32821 ggcgatcctc aaatccattt ccgagcaagg caaactcacc gatgatctgg cgaaggccat
    32881 caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca aacctaaacg
    32941 ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg acctgctgtg
    33001 gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg ccgataaagg
    33061 cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg aacggtttgc
    33121 cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg cgcatttggt
    33181 ttctacggtg gtgagcggta aagaagagga aggggcgaaa ttccgcgact atttcgatca
    33241 tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa
    33301 cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga
    33361 gagctattgc gagcaaatca tcatggatca ccttggcctg cgcctgaaca atgccccggc
    33421 ggatagctgg cgcaaaggcg tagtgagctg gacctggcgc atcaaggtgc tgatgcatct
    33481 ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt
    33541 tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg caacgatggg
    33601 cctcgatccg ggtctgcgta ctggggtaaa agtggcggtg gtcgatgcca ctggcaaact
    33661 ggtggcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag cgatgaccgt
    33721 tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg gtacagcttc
    33781 ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca
    33841 gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc tggcagcgca
    33901 ggagttcccg gatctcgacg tttcgctgcg tggtgcggta tctatcgccc gccgtttgca
    33961 ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag gtcagtatca
    34021 gcatgacgtc agccagacgc aactggcccg caaactggac gcagtagtag aagactgcgt
    34081 aaacgccgtt ggcgtcgatc tcaacactgc ttctgttccg ctgttaaccc gcgtggcggg
    34141 cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg gtcagttcca
    34201 gaaccgtcag caactgctga aagtgagccg tctggggccg aaagccttcg agcagtgcgc
    34261 gggcttcttg cgcattaacc atggtgataa cccgctggac gcgtctaccg tccacccgga
    34321 agcctatccg gtggtggaac gcattctggc agcaacacag caggcactga aagatctgat
    34381 gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg aaaaattcgg
    34441 tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc
    34501 ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg
    34561 tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg tcgatatcgg
    34621 cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg tagaagatcc
    34681 gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag tggatcttca
    34741 gcgtaaacgt atcgccctga ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg
    34801 tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac cgcgcggacg
    34861 tgaagcgcag cctgccggta acagcgcgat gatggacgcg ctggcggcag caatgggcaa
    34921 aaaacgttaa acgccctgat tgacccctaa acctgcgcga taaggtgtcc acgccgcatc
    34981 tggcaacgaa cgccttgtct gacataaaag tgccggagaa tatctccggc atttttattc
    35041 cacagccaaa ctcacaatat attccggcaa catttatcac ttcattaaca actgaaacct
    35101 taattaaaca ttagccagtc ctggtaattc gctattcgaa ttatattttc gctgcgatat
    35161 aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt cgcacacatt
    35221 ttaagtattg ctgatagaaa ccattctcat tatcattgtg ctgttgatta tttaatctct
    35281 ccttcgttgg caaatcatct ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc
    35341 cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc gtggaaaatc
    35401 actggctttt cccatgaaat cagcccggca tatcgccaaa aactgctttc tcttggcatg
    35461 ttacctggct cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc
    35521 gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt agaagtggaa
    35581 gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccatag gcttaattgg
    35641 taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac gtcagcgtgt
    35701 aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca ccaccgatca
    35761 tcaggtcacg ctggtggacc tgcccggcac ctattctctg accaccatct catcgcagac
    35821 ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg acctgctgat
    35881 taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctac aactgctgga
    35941 actcggcatt ccctgcatcg tggcactgaa catgctcgac attgccgaga agcaaaatat
    36001 tcgtattgaa attgatgctc tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc
    36061 aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata aagctaacga
    36121 gaatgtggaa ctggtgcatt acgcacagcc gctactcaac gaagcagatt cactggcaaa
    36181 agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga
    36241 aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg atgccgccct
    36301 cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac attgccgatg cgcgttacca
    36361 gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac ccagccgttt
    36421 caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt
    36481 tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac agccgctgtt
    36541 tgacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct acacgctcca
    36601 cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt
    36661 gctgccactg gtgccgcaga ttggcatgat gtacctgttc ctctccttcc ttgaggactc
    36721 cggctatatg gcgcgtgcgg cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc
    36781 tgggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg
    36841 cgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg caccgtttat
    36901 gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct tcgggcagaa
    36961 cggtgccctg gcggttttct cgctgtatat gctgggtatc gtgatggcgg tgctgactgg
    37021 cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc
    37081 ggtctaccat gtaccacacg ttaaaagcct gattatccag acctggcagc gtctgaaagg
    37141 cttcgttctg cgtgctggta aagtgattat catcgtcagc attttcctga gcgccttcaa
    37201 cagcttctcg ctgagcggaa aaatcgtcga taacatcaac gactcggcgc tggcttccgt
    37261 cagccgggtg atcaccccag tcttcaagcc gattggcgtg catgaagata actggcaggc
    37321 aacggttggc ctgtttacag gtgcaatggc gaaagaagtg gtggtgggca cgctcaacac
    37381 cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcagaat ttaacctcgg
    37441 tgaagagctg ttcagcgcgg tagatgaaac ctggcagagc ctgaaagaca ccttcagcct
    37501 tagcgtactg atgaatccca ttgaagcgag caaaggcgac ggcgaaatgg gtaccggggc
    37561 gatgggcgtg atggatcaga aattcggtag cgcagctgcc gcatacagct acctgatttt
    37621 cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg aatcaagccg
    37681 tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcac tggcaacatt
    37741 gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt gcattctggc
    37801 ggtaatcctg tttaacatcg tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga
    37861 tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg
    37921 tgattgccat taatggcttc acttattcag gtacgtgatt tgctggcgtt acggggccgt
    37981 atggaagcga cccagataag ccagacattg aacactccgc agccaatgat taacgccatg
    38041 ctgcaacagc tggaaagcat gggcaaagcc gtgcggattc aggaagaacc tgacggctgc
    38101 ctctctggca gttgtaaaag ctgcccggaa ggcaaagcct gtctgcgcga gtggtgggcg
    38161 ctgcgttaac catactccat cgcctgtttt gaaaagcccg gtatgcgttt gcatccgggc
    38221 ttttttgcgt gcggctttcc ataaaaatgc aattcttgca gcgcgacgta agttcctctg
    38281 aaagcgtctc gcagggatga aaactggcta atgcacagat gtggagtggc gcgtagagtc
    38341 gcggcattca aacaacaggt gaaggaacgc catgagtaaa aagcagagtt ccaccccaca
    38401 tgatgcgctg ttcaaactct ttttacgcca accggagacg gcacgcgatt ttcttgcgtt
    38461 tcatttaccg gcacccattc acgcgctctg tgatatgaaa accctcaagc tggagtcgag
    38521 cagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac
    38581 ggaacaagga ccaggataca tctattgtct gattgaacat caaagcacct caaacaaact
    38641 gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc ttgatgctgg
    38701 atacaaaacg ttgccgatgg tggtgccatt gttgttttac cacggtattg aaagccccta
    38761 tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggcaa ggcaacttta
    38821 tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca
    38881 ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg atctgatggg
    38941 gctggtagag caaatggcct gcttattaag tagtggatac gctaatgaca gacaaatcaa
    39001 agggctgttt aattacatac tgcaaactgg cgacgctgta cgttttaacg attttatcga
    39061 cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgactattg cggaaagatt
    39121 gcggcaggag ggggaacaat ccaaagccct gcatatagcc aaaataatgc ttgaatccgg
    39181 agtccctctt acagacatca tgcgctttac cggggtgtca gaagaagagt tggctgcggc
    39241 gagccagtaa atttctgtct cgccatttca aaagttgcct acaccctctg cttcaacgcc
    39301 accagcaggt gacaaaactc gaccggatgc gaaataaacg gcgcatgggc cgctttggcg
    39361 aagatatatg attcgctgtg aggccaaagt ttatccagca tcggcaccac tttgcgcggc
    39421 accagaccgt cgagatagcc atacaatcgc aaaaacggca tggacacgtt ttgcagcggc
    39481 tgacggagat cgaccgtttt caggatttcc agcccgccat taagcacgtc aacctccggc
    39541 atcggtaacg ccagaacggt tttcttcaac gcccgcgcat cctggcgcgc cgtttcagtc
    39601 cccatggttt gtaacgccag gaaccgctcc actgtacgct gaaaatcatc actgagttgc
    39661 tgctgaaatc ccgccagcac gtccggtttt atccccggcc actcgtcacg agcactaaaa
    39721 caaggtgacg acgccacggt gaccagcgcc tgaacacgct cgggatgggt taacgcaatc
    39781 tggcttgcca ccagcccgcc cagactccag cctaaccaaa tggctttatc aggtgcctgt
    39841 tgcagcacgg cttcggccat atcagcaagt gacagcgcac caaatccccg gctacgcccg
    39901 aagccgggca ggtcaacaag gtgcagcgta aaatgcgagc taagttcctc gtcaatgcaa
    39961 cgccacactt cggcattcag tccccatccg tgcagcagca caagatgaac attcccctga
    40021 cctttggtct gccaccagat gttattcatc cgctattgtt ctcttttgac ttacaaggat
    40081 gaacatatgc taacagtacc gggattatgc tggctatgcc gaatgccact ggcgttaggt
    40141 cattggggga tttgttcggt ctgttcacgc gccacccgca cagataaaac gttatgccca
    40201 caatgtggat tacccgccac acactcccat cttccctgcg gtcgctgcct gcaaaaaccg
    40261 ccgccctggc aaagactggt cacggttgcc gactatgcgc cgccgttaag tccgcttatc
    40321 caccagctta aattttcccg gcgcagtgaa atcgccagcg ccctgtcacg tctgttacta
    40381 ttggaagtct tacacgctcg tcgcaccacc gggttgcaat tgccggatcg catcgtcagc
    40441 gttccgttat ggcagcggcg tcactggcgt cggggattta atcagagcga tttgctgtgt
    40501 cagccgttat cacgctggtt gcactgccaa tgggatagcg aagccgtcac acgtacacgg
    40561 gccactgcga cccagcattt tctcagtgcc cggctgcgca agcgcaacct gaaaaatgcc
    40621 tttcgtcttg aattgcccgt gcaaggtcgc catatggtga ttgtggatga tgtcgttacc
    40681 accggaagta ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc ggcggctgtc
    40741 caggtctggt gcctttgtcg aaccttgtag agcctcgatg atgggcgtat tataaccaac
    40801 taaaatagtc aactattagg ccattactat gatccgtatt tccgatgctg cacaagcgca
    40861 ctttgccaaa ctgctggcaa atcaggaaga agggacacaa atccgcgtat ttgtgattaa
    40921 ccctggcacg cctaacgctg aatgtggcgt ttcttattgt ccgccggacg ctgtggaagc
    40981 caccgacaca gccctgaaat ttgacctgct gaccgcgtat gttgatgagt taagcgcacc
    41041 atacctggaa gatgcagaga tcgactttgt taccgaccag ttgggttctc agttaacgct
    41101 gaaagccccg aacgccaaaa tgcgtaaagt ggcagacgat gcaccgctga tggagcgcgt
    41161 ggagtatatg ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg gtcgcgtttc
    41221 gctgatggaa atcaccgaag acggttacgc cattctgcaa tttggcggcg gctgtaacgg
    41281 ttgttccatg gtcgatgtga cgctgaaaga agggatcgag aagcagctgc tgaacgaatt
    41341 cccggagctg aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac actcctacta
    41401 ctaagttatc ccctcttttg tagattgccc gatgcgacgc taaagcgtca tttcgggcct
    41461 acgttgaaca tgtgccagac gttggcgcag cattgctttg gcaaatccct cccttcgttt
    41521 ctccgcgata atatgaccaa cctctcataa tttaaattta ccccgctctg gtgattctca
    41581 aacgccagat gttacccgta tcattcacat gggtaccaaa catactcctg acatctgact
    41641 acaataatta gttttagtgg gtatcagtcg tggtgccgca atatctctgt tcccgattgg
    41701 gataattaga gtttgtcgtc agaaaattga cgttacccat aacaaatgaa aggccaggta
    41761 aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc tgatgatccg
    41821 cttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgctgttg gattaatgca
    41881 aggaatgccg ctggataaag ttattggctc catcaaagcc ggtgtcggcg ggacgctcgg
    41941 tagccttgcc ctgatcatgg gttttggcgc aatgctgggc aaaatgctgg cagactgcgg
    42001 tggcgcacaa cgtatcgcca ccacgctgat tgccaaattt ggtaaaaagc acatccagtg
    42061 ggcggtggtg ttaaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggcttcgt
    42121 gctgatgctg cctctggtgt ttaccatcgc ggcttccgcg aatattccac tgctgtatgt
    42181 tggtgtacca atggcggctg cactgtctgt gacccacggc ttcctgccac cgcatccggg
    42241 cccgactgcg attgccacca ttttcaatgc cgatatgggt aaaaccctgc tgtacggtac
    42301 tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgttgaaagg
    42361 tatcgataag ccaatcccgg aaggtctcta cagcgcgaaa accttcagcg aagaagagat
    42421 gccgagcttt ggcgtcagcg tctggacttc tctggtgccg gttgtgctga tggcgatgcg
    42481 tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct
    42541 cggtgacccg gtaatggcaa cgctgattgc cgtgctgatt gcgatgttca cctttggtct
    42601 gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat
    42661 tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag
    42721 cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat
    42781 ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg ttgcggcaat
    42841 cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cagagctgat
    42901 ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc cgggcttctg
    42961 gctgttcaaa gagtacttta acctgactat cggcgagacc atcaaatcct ggtcgatgct
    43021 ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg
    43081 ataacactga ctgccggatg catcgcatcc ggcatcaacc gcactctact tcttcttcgc
    43141 tgcagctctg cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat catcggcaaa
    43201 catcgtctca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt aactggtgcc
    43261 aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc ctaacagagc
    43321 actgttactg tcggcaatgt agcgctgcaa accacggttc agcgtcggcg tcatcgacat
    43381 caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca gtgcatccag
    43441 cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca cttcatccgg
    43501 atacagcccc agggttttgc ccagcgttag atccccgctc tcccagtaac cgcgtagcgt
    43561 aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt tcggtgcacg
    43621 gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca caccgctgct
    43681 gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa tcaccataca
    43741 gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg gatagtgaac
    43801 atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca gcatcgacat
    43861 cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca gctcgataaa
    43921 cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc cccagttctg
    43981 ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc aatatagttc
    44041 acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat cacgatacaa
    44101 gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact ggctgtaagc
    44161 cagccactgc aaccagagat aaaaatcgac gtcattacga tgttcttcgc agaactgacg
    44221 cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc cccagcgcat
    44281 ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc agaacaggct
    44341 gtcgccctgc tctgcaacaa actggcgaaa cgcggtcatc tgctcatcat cacgttgcgc
    44401 gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg tggagtaatc
    44461 gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca actgccacca
    44521 ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga tataaatcac
    44581 attcagccaa cggcgagaag acgggctgta tgggctggca ctctccggat ttgccggata
    44641 gagcgcatga atcgggttca ggccgatgaa cgacccgcca cgttttgcca catccaccag
    44701 catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc gcagcgtata
    44761 aagctgaacg caggcacccc acagcttttg tttattcagc aacgcctgcg gttcgtaaca
    44821 gcgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg tgagtgtcag
    44881 cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc ccgttacatg
    44941 gcctttgtac tgtgttcctt cttcggtggt cagcagccag ctatattcgc cgctgccctc
    45001 caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga ctggcgttac
    45061 cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt tggtttcggc
    45121 gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc ccgccgccag
    45181 cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg cctgccagat
    45241 acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc gggcggtatt
    45301 gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca cctgcttttg
    45361 tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc cttgtttgcc
    45421 gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt taccgctttc
    45481 cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca ccgggtcgta
    45541 gcctttggcc agaatagcct tcacttgttc cacggtatga ccaaaaataa agatattttc
    45601 ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga cagtaagcgc
    45661 accattgagc gccagtttca tattgccggt accggaagct tctttacctg cagtcgaaat
    45721 ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc aataatccgg
    45781 caggaacacc accttcaact tatcgccaac cagtggatcg ttgttgatca cgtcagccac
    45841 tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg ctttcgcgcc
    45901 gaagaggaag acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt ctttgtacaa
    45961 cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac gtttgatctg
    46021 aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca caaactccgc
    46081 cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt tcgcatcatc
    46141 agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt tttgcagtga
    46201 tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac gtggggtaat
    46261 accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg ggaacagatc
    46321 tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac cgccaaccac
    46381 acacaggttc gccatatgca cttgtttatc gtgcaccacc gccagtttgg cccacacttt
    46441 ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa tttcgttaat
    46501 aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc gttccagcgc
    46561 ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatgg cccaggcgtc
    46621 atcccagctc atctggtgct catcgatcag cacgcgcagc agttctggaa tcgcgatagt
    46681 tgggtgggta tcgttcagct gaataacttc gtaatccgcc agttcgtgca gtttacgccc
    46741 cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga agtactgctg
    46801 catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa ctttggtcag
    46861 tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt taaatttagt
    46921 cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg ccacgccatt
    46981 acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact ccggctccca
    47041 gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg cttcgttgtg
    47101 gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct gtttgccatc
    47161 gacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg tcgcagactg
    47221 accgacagtt gccattgagt cgaggaagca cgccgccaga cgtcccagac caccgttacc
    47281 cagcgccggg tcgatctctt cttccagcag gtccgtcaga ttgatgtcat aagccttcaa
    47341 cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg tcaggcgacc
    47401 aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg gcttggcgaa
    47461 tggctgagca cgcagcattt cggccagtgc ttcactcact gctagccacc actggcgagg
    47521 agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa gcgcttcctg
    47581 aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct tagattttca
    47641 acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc tgcgggggat
    47701 taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcaaccac ttaaagctag
    47761 tgcaaaccac aggattagca gcaaatcaat gcaatacagc gcagaaaatc tgtatctaag
    47821 tgcaaaaaat ggccgttgcg tattttcaaa aagcggaagg taactctata aattaagtaa
    47881 aggagtgaaa cagtttcata agtaaaatat ccagtgtgct ccatctcatt cttaatagat
    47941 ttattaagat catcttttta gatggcactt tcatcaggaa tgaagaagaa acccttgctt
    48001 aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg
    48061 tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac
    48121 gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca
    48181 aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa
    48241 aactaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac
    48301 tttccggcgc gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga
    48361 ccaccctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg
    48421 aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa
    48481 ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga
    48541 cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg
    48601 tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta
    48661 ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg
    48721 gcattgccaa tctgcgtgtt cgtgaccaac tgctggaaat tggcagtcag caactggcat
    48781 ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg
    48841 cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg
    48901 ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc ctagcgggaa
    48961 tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg
    49021 caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca
    49081 cccgtgtgac cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc
    49141 tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca
    49201 acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg
    49261 ccgccgcaga aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg
    49321 cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg agtttgttca
    49381 accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc
    49441 tggaaaatcc gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg
    49501 gcgaagttaa caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca
    49561 ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg
    49621 atgaagcgga acggctggca aaactggcac tggaagagct gccgccgggc tggttctata
    49681 gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc
    49741 gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac gtctggcact
    49801 acgccttatg gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa
    49861 ccgcgtggga aacacaggaa aaagcgttcc agctgatcaa cgagcaacat ctggaacagc
    49921 tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc
    49981 tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac
    50041 agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag
    50101 ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca
    50161 gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata
    50221 aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact
    50281 tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac
    50341 ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg
    50401 atctcaaccg taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg
    50461 acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagcc
    50521 attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caactcaata
    50581 cgctgccgga actggaacag catcgcgcac agcgtattct gcgagaaatc aatcaacatc
    50641 atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgctg aatcatccgg
    50701 aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc
    50761 tgatttactc cggttacagc aatgagcaaa tagcgggtga actggaagtg gcggcaacca
    50821 ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg
    50881 ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg
    50941 ataatgcatt ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca
    51001 tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc
    51061 agcagatggc atgaaggatg ggcgacctta aattctcccg cgcccgccag cgccatcggc
    51121 agtaccagtt ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc
    51181 acctctttca ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg
    51241 aaaaagcgtt cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc
    51301 tggtcgcgcg gcaggttatg aatattctgt tcatgcaggg aaaaactccc cgccagtgta
    51361 gcgatttcac gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc
    51421 tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac gggcgagact
    51481 tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc
    51541 tgatgaattc ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc
    51601 ggggccgacg ggttatcggt gcctccgctc acttcaacac gcgaaggtcc atcggcaaac
    51661 cacagcgcgg gcagcaccgt ttgcagcacc agcgtacagc ttccggcact accgatagca
    51721 aagcggtact cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgaccccagc
    51781 tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg cttttaccgc ggtcagatgc
    51841 tggcgcaaca gccccggttt cgcccgcccg gcacgaatgc tggtgatggt aaatggctgg
    51901 ccggttatca tcgacaggct cagcgccgag cgcaggatct gcccgccacc ttcgccctgt
    51961 gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac cacctgacgc
    52021 agggtataga taacttccac cagatcgctt tgtgccgcca tcaccgcatc aatatcttta
    52081 tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac atgcgcggtg
    52141 gcacgaattt gatcttccac gctgaacagt tttttcgctt tagtacggct cattacccgc
    52201 ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg gacgataaag
    52261 ctttttgctc ccatcgaacc gggaataatt ccatattgac cagcacgcgc agacaccgcg
    52321 cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac atagttgtgg
    52381 tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt ctgcgtaatg
    52441 ctctgcaacg ccgttaccac gttttccatc atcgcatcgc ggttaaggct ggcgaaaagc
    52501 tgcgcccagg ccacggctct taggtaatca tcaaagtatt ccgtaccttc cataaagtac
    52561 gccaggtcgc gcgatggcaa cgtttcaagc tggtcctgca tctctttttg tgccaaatcg
    52621 ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac cggagtgcag cataatccac
    52681 acctggtctg actcatcaag gcagatttca ataaagtggt tacccgttcc cagcgttccc
    52741 aggtgtttat agttgttagt gttaaggaaa cgcggatatt tttgcgttaa ccactgataa
    52801 ccggcttcaa gctcagccca tttcgcatcg acgttaacag gcgggttttc ccatgcgcct
    52861 ttatcacgtt tacaacggcc agtggtacgc ccgtgcggca cggccatttc aatcgcctga
    52921 cgcagctctg ccaggttttc aggcaggtct tccgccgtta acgcggtacg cagcgcgttc
    52981 attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt ggtcgggatc
    53041 acgctaccaa tggtggaacc tttacccagg tgtacatcag gcattaccgc aatatgtttg
    53101 aaaataaacg gcatcttcgc cgtattaata agttgctgac gctcatcggc ttctaccggc
    53161 acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc gtaattcatt
    53221 ttgtttttct cttttcgttg tttgctgtcg tgataattgc aaccgtcgtg ccag
//
LOCUS       DAZFVO020000012        51635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFVO020000012 DAZFVO020000000
VERSION     DAZFVO020000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.012"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(115..1479)
                     /gene="mnmE"
                     /locus_tag="ACHKNB_RS03140"
                     /old_locus_tag="pgaptmp_000628"
     CDS             complement(115..1479)
                     /gene="mnmE"
                     /locus_tag="ACHKNB_RS03140"
                     /old_locus_tag="pgaptmp_000628"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGU4194204.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1585..3231)
                     /gene="yidC"
                     /locus_tag="ACHKNB_RS03145"
                     /old_locus_tag="pgaptmp_000629"
     CDS             complement(1585..3231)
                     /gene="yidC"
                     /locus_tag="ACHKNB_RS03145"
                     /old_locus_tag="pgaptmp_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGU4194205.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3234..3491)
                     /gene="yidD"
                     /locus_tag="ACHKNB_RS03150"
                     /old_locus_tag="pgaptmp_000630"
     CDS             complement(3234..3491)
                     /gene="yidD"
                     /locus_tag="ACHKNB_RS03150"
                     /old_locus_tag="pgaptmp_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGU4194206.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3455..3814)
                     /gene="rnpA"
                     /locus_tag="ACHKNB_RS03155"
                     /old_locus_tag="pgaptmp_000631"
     CDS             complement(3455..3814)
                     /gene="rnpA"
                     /locus_tag="ACHKNB_RS03155"
                     /old_locus_tag="pgaptmp_000631"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGU4194207.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3831..3971)
                     /gene="rpmH"
                     /locus_tag="ACHKNB_RS03160"
                     /old_locus_tag="pgaptmp_000632"
     CDS             complement(3831..3971)
                     /gene="rpmH"
                     /locus_tag="ACHKNB_RS03160"
                     /old_locus_tag="pgaptmp_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGU4194208.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4201..4281)
                     /locus_tag="ACHKNB_RS03165"
                     /old_locus_tag="pgaptmp_000633"
     CDS             complement(4201..4281)
                     /locus_tag="ACHKNB_RS03165"
                     /old_locus_tag="pgaptmp_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194209.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4578..5981
                     /gene="dnaA"
                     /locus_tag="ACHKNB_RS03170"
                     /old_locus_tag="pgaptmp_000634"
     CDS             4578..5981
                     /gene="dnaA"
                     /locus_tag="ACHKNB_RS03170"
                     /old_locus_tag="pgaptmp_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGU4194210.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5986..7086
                     /gene="dnaN"
                     /locus_tag="ACHKNB_RS03175"
                     /old_locus_tag="pgaptmp_000635"
     CDS             5986..7086
                     /gene="dnaN"
                     /locus_tag="ACHKNB_RS03175"
                     /old_locus_tag="pgaptmp_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGU4194211.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7086..8159
                     /gene="recF"
                     /locus_tag="ACHKNB_RS03180"
                     /old_locus_tag="pgaptmp_000636"
     CDS             7086..8159
                     /gene="recF"
                     /locus_tag="ACHKNB_RS03180"
                     /old_locus_tag="pgaptmp_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGU4194212.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8188..10602
                     /gene="gyrB"
                     /locus_tag="ACHKNB_RS03185"
                     /old_locus_tag="pgaptmp_000637"
     CDS             8188..10602
                     /gene="gyrB"
                     /locus_tag="ACHKNB_RS03185"
                     /old_locus_tag="pgaptmp_000637"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGU4194213.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10842..11240
                     /gene="yidB"
                     /locus_tag="ACHKNB_RS03190"
                     /old_locus_tag="pgaptmp_000638"
     CDS             10842..11240
                     /gene="yidB"
                     /locus_tag="ACHKNB_RS03190"
                     /old_locus_tag="pgaptmp_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HGU4194214.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11355..12167
                     /gene="yidA"
                     /locus_tag="ACHKNB_RS03195"
                     /old_locus_tag="pgaptmp_000639"
     CDS             11355..12167
                     /gene="yidA"
                     /locus_tag="ACHKNB_RS03195"
                     /old_locus_tag="pgaptmp_000639"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGU4194215.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12213..12869)
                     /gene="yidX"
                     /locus_tag="ACHKNB_RS03200"
                     /old_locus_tag="pgaptmp_000640"
     CDS             complement(12213..12869)
                     /gene="yidX"
                     /locus_tag="ACHKNB_RS03200"
                     /old_locus_tag="pgaptmp_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HGU4194216.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13147..13836
                     /gene="dgoR"
                     /locus_tag="ACHKNB_RS03205"
                     /old_locus_tag="pgaptmp_000641"
     CDS             13147..13836
                     /gene="dgoR"
                     /locus_tag="ACHKNB_RS03205"
                     /old_locus_tag="pgaptmp_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HGU4194217.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13833..14711
                     /gene="dgoK"
                     /locus_tag="ACHKNB_RS03210"
                     /old_locus_tag="pgaptmp_000642"
     CDS             13833..14711
                     /gene="dgoK"
                     /locus_tag="ACHKNB_RS03210"
                     /old_locus_tag="pgaptmp_000642"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HGU4194218.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14695..15312
                     /gene="dgoA"
                     /locus_tag="ACHKNB_RS03215"
                     /old_locus_tag="pgaptmp_000643"
     CDS             14695..15312
                     /gene="dgoA"
                     /locus_tag="ACHKNB_RS03215"
                     /old_locus_tag="pgaptmp_000643"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HGU4194219.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15309..16457
                     /gene="dgoD"
                     /locus_tag="ACHKNB_RS03220"
                     /old_locus_tag="pgaptmp_000644"
     CDS             15309..16457
                     /gene="dgoD"
                     /locus_tag="ACHKNB_RS03220"
                     /old_locus_tag="pgaptmp_000644"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HGU4194220.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHVDNSVAEW"
     gene            16532..17869
                     /gene="dgoT"
                     /locus_tag="ACHKNB_RS03225"
                     /old_locus_tag="pgaptmp_000645"
     CDS             16532..17869
                     /gene="dgoT"
                     /locus_tag="ACHKNB_RS03225"
                     /old_locus_tag="pgaptmp_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194221.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYYIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17866..18930)
                     /gene="cbrA"
                     /locus_tag="ACHKNB_RS03230"
                     /old_locus_tag="pgaptmp_000646"
     CDS             complement(17866..18930)
                     /gene="cbrA"
                     /locus_tag="ACHKNB_RS03230"
                     /old_locus_tag="pgaptmp_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HGU4194222.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            19031..20245
                     /gene="yidR"
                     /locus_tag="ACHKNB_RS03235"
                     /old_locus_tag="pgaptmp_000647"
     CDS             19031..20245
                     /gene="yidR"
                     /locus_tag="ACHKNB_RS03235"
                     /old_locus_tag="pgaptmp_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HGU4194223.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGLVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20247..20579)
                     /gene="yidQ"
                     /locus_tag="ACHKNB_RS03240"
                     /old_locus_tag="pgaptmp_000648"
     CDS             complement(20247..20579)
                     /gene="yidQ"
                     /locus_tag="ACHKNB_RS03240"
                     /old_locus_tag="pgaptmp_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4194224.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20819..20891
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20885..21298
                     /gene="ibpA"
                     /locus_tag="ACHKNB_RS03245"
                     /old_locus_tag="pgaptmp_000649"
     CDS             20885..21298
                     /gene="ibpA"
                     /locus_tag="ACHKNB_RS03245"
                     /old_locus_tag="pgaptmp_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HGU4194225.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21410..21838
                     /gene="ibpB"
                     /locus_tag="ACHKNB_RS03250"
                     /old_locus_tag="pgaptmp_000650"
     CDS             21410..21838
                     /gene="ibpB"
                     /locus_tag="ACHKNB_RS03250"
                     /old_locus_tag="pgaptmp_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HGU4194226.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22035..23696
                     /gene="yidE"
                     /locus_tag="ACHKNB_RS03255"
                     /old_locus_tag="pgaptmp_000651"
     CDS             22035..23696
                     /gene="yidE"
                     /locus_tag="ACHKNB_RS03255"
                     /old_locus_tag="pgaptmp_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HGU4194227.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23693..24409)
                     /gene="yidP"
                     /locus_tag="ACHKNB_RS03260"
                     /old_locus_tag="pgaptmp_000652"
     CDS             complement(23693..24409)
                     /gene="yidP"
                     /locus_tag="ACHKNB_RS03260"
                     /old_locus_tag="pgaptmp_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4194228.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24705..26321
                     /locus_tag="ACHKNB_RS03265"
                     /old_locus_tag="pgaptmp_000653"
     CDS             24705..26321
                     /locus_tag="ACHKNB_RS03265"
                     /old_locus_tag="pgaptmp_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4194229.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEAFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26321..27135
                     /locus_tag="ACHKNB_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /pseudo
     CDS             26321..27135
                     /locus_tag="ACHKNB_RS03270"
                     /old_locus_tag="pgaptmp_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27132..28025)
                     /gene="yidL"
                     /locus_tag="ACHKNB_RS03275"
                     /old_locus_tag="pgaptmp_000655"
     CDS             complement(27132..28025)
                     /gene="yidL"
                     /locus_tag="ACHKNB_RS03275"
                     /old_locus_tag="pgaptmp_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4194230.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLTPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28192..29907
                     /gene="yidK"
                     /locus_tag="ACHKNB_RS03280"
                     /old_locus_tag="pgaptmp_000656"
     CDS             28192..29907
                     /gene="yidK"
                     /locus_tag="ACHKNB_RS03280"
                     /old_locus_tag="pgaptmp_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HGU4194231.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGSPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29904..31397
                     /gene="yidJ"
                     /locus_tag="ACHKNB_RS03285"
                     /old_locus_tag="pgaptmp_000657"
     CDS             29904..31397
                     /gene="yidJ"
                     /locus_tag="ACHKNB_RS03285"
                     /old_locus_tag="pgaptmp_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4194232.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGTHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31444..31893)
                     /gene="yidI"
                     /locus_tag="ACHKNB_RS03290"
                     /old_locus_tag="pgaptmp_000658"
     CDS             complement(31444..31893)
                     /gene="yidI"
                     /locus_tag="ACHKNB_RS03290"
                     /old_locus_tag="pgaptmp_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HGU4194233.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32003..32350
                     /gene="yidH"
                     /locus_tag="ACHKNB_RS03295"
                     /old_locus_tag="pgaptmp_000659"
     CDS             32003..32350
                     /gene="yidH"
                     /locus_tag="ACHKNB_RS03295"
                     /old_locus_tag="pgaptmp_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HGU4194234.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32340..32702
                     /gene="yidG"
                     /locus_tag="ACHKNB_RS03300"
                     /old_locus_tag="pgaptmp_000660"
     CDS             32340..32702
                     /gene="yidG"
                     /locus_tag="ACHKNB_RS03300"
                     /old_locus_tag="pgaptmp_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HGU4194235.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32699..33196
                     /gene="yidF"
                     /locus_tag="ACHKNB_RS03305"
                     /old_locus_tag="pgaptmp_000661"
     CDS             32699..33196
                     /gene="yidF"
                     /locus_tag="ACHKNB_RS03305"
                     /old_locus_tag="pgaptmp_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HGU4194236.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33204..34388)
                     /gene="emrD"
                     /locus_tag="ACHKNB_RS03310"
                     /old_locus_tag="pgaptmp_000662"
     CDS             complement(33204..34388)
                     /gene="emrD"
                     /locus_tag="ACHKNB_RS03310"
                     /old_locus_tag="pgaptmp_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HGU4194237.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSDQGQ
                     PV"
     gene            34470..34544
                     /gene="ysdE"
                     /locus_tag="ACHKNB_RS03315"
                     /old_locus_tag="pgaptmp_000663"
     CDS             34470..34544
                     /gene="ysdE"
                     /locus_tag="ACHKNB_RS03315"
                     /old_locus_tag="pgaptmp_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HGU4194238.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34807..34896)
                     /gene="tisB"
                     /locus_tag="ACHKNB_RS03320"
                     /old_locus_tag="pgaptmp_000664"
     CDS             complement(34807..34896)
                     /gene="tisB"
                     /locus_tag="ACHKNB_RS03320"
                     /old_locus_tag="pgaptmp_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HGU4194239.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35461..35559
                     /gene="ivbL"
                     /locus_tag="ACHKNB_RS03325"
                     /old_locus_tag="pgaptmp_000665"
     CDS             35461..35559
                     /gene="ivbL"
                     /locus_tag="ACHKNB_RS03325"
                     /old_locus_tag="pgaptmp_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HGU4194240.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35665..37353
                     /gene="ilvB"
                     /locus_tag="ACHKNB_RS03330"
                     /old_locus_tag="pgaptmp_000666"
     CDS             35665..37353
                     /gene="ilvB"
                     /locus_tag="ACHKNB_RS03330"
                     /old_locus_tag="pgaptmp_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGU4194241.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37357..37647
                     /gene="ilvN"
                     /locus_tag="ACHKNB_RS03335"
                     /old_locus_tag="pgaptmp_000667"
     CDS             37357..37647
                     /gene="ilvN"
                     /locus_tag="ACHKNB_RS03335"
                     /old_locus_tag="pgaptmp_000667"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4194242.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37722..38312
                     /gene="uhpA"
                     /locus_tag="ACHKNB_RS03340"
                     /old_locus_tag="pgaptmp_000668"
     CDS             37722..38312
                     /gene="uhpA"
                     /locus_tag="ACHKNB_RS03340"
                     /old_locus_tag="pgaptmp_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HGU4194243.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38312..39814
                     /gene="uhpB"
                     /locus_tag="ACHKNB_RS03345"
                     /old_locus_tag="pgaptmp_000669"
     CDS             38312..39814
                     /gene="uhpB"
                     /locus_tag="ACHKNB_RS03345"
                     /old_locus_tag="pgaptmp_000669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HGU4194244.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39824..41143
                     /gene="uhpC"
                     /locus_tag="ACHKNB_RS03350"
                     /old_locus_tag="pgaptmp_000670"
     CDS             39824..41143
                     /gene="uhpC"
                     /locus_tag="ACHKNB_RS03350"
                     /old_locus_tag="pgaptmp_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HGU4194245.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            41281..42672
                     /gene="uhpT"
                     /locus_tag="ACHKNB_RS03355"
                     /old_locus_tag="pgaptmp_000671"
     CDS             41281..42672
                     /gene="uhpT"
                     /locus_tag="ACHKNB_RS03355"
                     /old_locus_tag="pgaptmp_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HGU4194246.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42717..44483)
                     /gene="adeD"
                     /locus_tag="ACHKNB_RS03360"
                     /old_locus_tag="pgaptmp_000672"
     CDS             complement(42717..44483)
                     /gene="adeD"
                     /locus_tag="ACHKNB_RS03360"
                     /old_locus_tag="pgaptmp_000672"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HGU4194247.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            44658..45992
                     /gene="adeQ"
                     /locus_tag="ACHKNB_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /gene_synonym="yicO"
     CDS             44658..45992
                     /gene="adeQ"
                     /locus_tag="ACHKNB_RS03365"
                     /old_locus_tag="pgaptmp_000673"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HGU4194248.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            46045..46497
                     /gene="yicN"
                     /locus_tag="ACHKNB_RS03370"
                     /old_locus_tag="pgaptmp_000674"
     CDS             46045..46497
                     /gene="yicN"
                     /locus_tag="ACHKNB_RS03370"
                     /old_locus_tag="pgaptmp_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HGU4194249.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            46708..47898
                     /gene="nepI"
                     /locus_tag="ACHKNB_RS03375"
                     /old_locus_tag="pgaptmp_000675"
     CDS             46708..47898
                     /gene="nepI"
                     /locus_tag="ACHKNB_RS03375"
                     /old_locus_tag="pgaptmp_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HGU4194250.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(47939..48232)
                     /gene="yicS"
                     /locus_tag="ACHKNB_RS03380"
                     /old_locus_tag="pgaptmp_000676"
     CDS             complement(47939..48232)
                     /gene="yicS"
                     /locus_tag="ACHKNB_RS03380"
                     /old_locus_tag="pgaptmp_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HGU4194251.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            48454..49272
                     /gene="nlpA"
                     /locus_tag="ACHKNB_RS03385"
                     /old_locus_tag="pgaptmp_000677"
     CDS             48454..49272
                     /gene="nlpA"
                     /locus_tag="ACHKNB_RS03385"
                     /old_locus_tag="pgaptmp_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HGU4194252.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(49276..50199)
                     /gene="yicL"
                     /locus_tag="ACHKNB_RS03390"
                     /old_locus_tag="pgaptmp_000678"
     CDS             complement(49276..50199)
                     /gene="yicL"
                     /locus_tag="ACHKNB_RS03390"
                     /old_locus_tag="pgaptmp_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HGU4194253.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(50310..51494)
                     /gene="setC"
                     /locus_tag="ACHKNB_RS03395"
                     /old_locus_tag="pgaptmp_000679"
     CDS             complement(50310..51494)
                     /gene="setC"
                     /locus_tag="ACHKNB_RS03395"
                     /old_locus_tag="pgaptmp_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HGU4194254.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT        12029 a        13048 c        13891 g        12667 t
ORIGIN      
        1 attataaatt acgtaagaat tttaatgttt aaaaaaaggg ttgaggaata acaggagtgc
       61 taacaatgaa gcgaagccgc atctgacagt cagaatacgg cttcgtaagc gcggttactt
      121 accaatacag aagctggaga aaatccgccc cagcaggtcg tctgaagtaa attccccggt
      181 gatttcgctt aagttctgct gtgccagacg caactcttcc gccagcagtt cacctgccca
      241 ggctcccaac agttgcgctt tgccctgttg tagatgttcc gctgcctgtt ccagcgcctg
      301 taggtggcga cgacgcgcca ggaagccgcc ttccatgttg gtgtcaaagc ccatgctctg
      361 tttgagatgg ttacgcagca cgtccacgcc ttcaccagtc cttgccgaga gacgaattaa
      421 cgcgtgaccg ttcacttcac tcattcccag cgtttcgccg gtgatatcgg ctttattgcg
      481 caccacggtg atcggcagtt tcgctggcag acgggcaata aattccggcc agatctctgc
      541 cggatccacg gcgtctgttg tggtgccatc gaccataaac agcacgcggt cggcctgttc
      601 aatttcctgc cacgcgcgct cgataccaat acgttccact tcgtcactgg cttcacgtag
      661 cccagcggta tcgatgatat gcagcggcat tccgtcaatg tggatatgct cacgcagcac
      721 gtcacgtgtg gttccggcga tatcagttac gattgctgct tcacggcccg ccagcgcgtt
      781 taacaggctc gatttaccgg cgttaggacg tccggcaatc accaccttca tcccttcgcg
      841 caacaaacta ccctgacgtg cttcagcacg cactgcatcg agatcggcaa tcacgtcatt
      901 aagctgggct tcaattttgc cgtcggagag gaaatcgatc tcttcatcag ggaaatcaat
      961 tgccgcttcg acgtaaatgc gcaagtgggt gagggcttcc accagatggt tgacccgtgc
     1021 ggagaatgcg ccttgcagcg agttaagtgc cgaacgggcc gcctgttccg aactggcgtc
     1081 gataagatcg gcaatcgcct cggcctgggc taagtcgagt ttatcgttaa gaaacgcgcg
     1141 ttcggaaaac tcaccagggc gagcaatccg caggccggga atggtcagaa tgcgttttaa
     1201 cagcaggtcg aggatcaccg gaccgccatg accttgcagt tccagcacat cttcgccggt
     1261 gaacgagttc gggccaggga accatagcgc aatcccctga tcgagcacgc tgccgtcggc
     1321 gtctttaaac ggaagataat cggcgtagcg cggcttaggt agtttaccca gcacggtttc
     1381 ggcaacttca cgggctttga agccggagat gcgcaggatg ccaacgccgc cacgtcccgg
     1441 aggcgtggcc tgggctacga tagtgtcatt atcgctcatg atgttcctgt tgctttgtgt
     1501 ggcggatgcg cggtgcttat ccgccctacg aaaagaaaaa aggcggtcaa ctgaccgccc
     1561 ttattttagc gaaaactcac cgaatcagga ttttttcttc tcgcggctat gcaggccacg
     1621 tttttccaga ccacggtaaa tcagctgctg ctgaataatg gttaccaggt tgctgacgat
     1681 atagtacagc accagacctg acgggaacca caggaagaac acggtgaaga tgaccggcat
     1741 aaaggtcatg atcttctgct gcatcgggtc ggtcactgtg gtcggcgaca tcttctgaat
     1801 gaagaacatc gttacgccca tcaggatcgg caagatgtag tacgggtcct gtgccgacag
     1861 gtcgtggatc cacagtgcaa acggtgcctg acgcagttca acggaaccca tcagcatgta
     1921 gtacaacgcc aggaagattg gcatctggat cagcagcggg aagcagccgc ccagcgggtt
     1981 aaccttctca gctttgtaca gcgccatcat ttcctggctg atacgctgtt tgtcatcgcc
     2041 cagacgctca cgcattgcct gaatcttcgg ctgcagcata cgcatcttcg ccatggaggt
     2101 gtactgcgct ttggtcagcg ggtacatgat gccacgaacg ataaaggtga tgatgataat
     2161 ggagaagccc cagttaccca caaagctatg gatccatttc agcagtttga acagcggctg
     2221 agagatgaac cacaaccaac cgtaatcaac ggtcagatcc aggtgcggag caacagctgc
     2281 cattttgtcc tggatttccg ggccaaccca cagggtgctg ttcatcgcgc cagtctgacc
     2341 aggctgaacc agtaccggct gagatttata gccgatagcg gcgatgccgt tacccagatt
     2401 agcggtatag aagttgttgg taccgtcgtt atgcgggatc cacgccgtcg cgaaatactg
     2461 ttgcagcatc gccacccaac cacctttcga agagatgttc aggttttcgt tatcggcaat
     2521 ggtatcgaac ttgtatttct cgtacttctc gtcaggcgtg gagtacgccg cgccgcggaa
     2581 ggtgtgcagt gcgaagttgc tgcttccggt atcgagatgc ggtggcagag tgatggattg
     2641 cttcaactga ccaaaggtgg agatttccag cggtttctcg ccagcgttct gcacgttgta
     2701 gttgacgttg acagcgtaat caccacgttt caggacaaac gttttggtaa acgtgttgcc
     2761 tgccgcgtcg gtatacgtca tcggcacctg cagttcgttt tgaccttcag ccagcacata
     2821 agcgtctttt tcaacgttat acagcggacg cgggccgtta gccgggttat ccgggccatc
     2881 acgaccggtc agaccgctct gtgcctgata aataaactgc ggtgaagttt ccagcagctg
     2941 gaacggctgg gtagagttca gctctttcgg gtaagcaggc agcagagctt gctcaacatc
     3001 accaccacgg gtgttgatgg tcagatcaag cacgtcggtc ttaaccgaga tcagtttccc
     3061 ctggccactg gccggtacgc cctggtcggc ggcgctaccc gctgcggtgg tcgttgtctg
     3121 cgtggtctgt tgggcctgag gttgcgggtt tttatcctgc tcccaggctt gccagatcat
     3181 gaaagacacg aacagcaaag cgatgactaa aagattgcgt tgcgaatcca tcgttagtgt
     3241 tctctggtat caaatggtcc gggcgggacg ggatcgtcac caccagggtg taaagggtgg
     3301 cattttaata cgcgtttcac cgtcaaccaa ctgcctttta tcactccaaa cctgcgcaat
     3361 gcctcaattc cgtagcttga acaggttgga gtgaaacgac aatgcggccc gagtagcgga
     3421 ctaatcaggc gttgatagac ccgaatgagg gctatcagga cccgcgagcc aggcgacagt
     3481 ggcggcgcca taatttttcc aacgcttccg agagagcacg gttatcgagg tcggcaaccc
     3541 cttttttcgc caccaccacg aaatccatag ccgggagttc atgttggcgc agacggaagc
     3601 tttcacgcgt cagacgttta atccgattgc gttcatgggc gcgtcgaacg tttttcttgg
     3661 cgactgtaag accgatacgg ggatgcccca gcgaattcag gcggccgaga atggtaattt
     3721 gcggcgtgcc agcccgttgt ggctgctgga agacgaatgt gaattgactg ggagttaaca
     3781 agcgtaactc cctgggaaat gcgagcttaa ccactcaggg gttagcttta ttacttagaa
     3841 acggtcagac gagcgcggcc tttagcacga cgacgtgcca gaacctgacg accattttta
     3901 gtagccatac gagcacggaa gccgtgagaa cggttgcgct tcagtacaga cggttgaaaa
     3961 gtgcgtttca tggcgatttc tacctaaact tgaataaatt caatggcttt attggatatc
     4021 cgccgaaaaa tgaaacgatg gacaccgaag ccatgggtga ttaaagaggc cggattgtaa
     4081 taattgtaca ctccggagtc aattctcttt ccttatttac cgcgcttttc cgcacctttt
     4141 cgcagggaaa atgtacgacc tcacaccagt ggaaaccagc atggcgcgcc gggtggagga
     4201 ttatacgggc tgatgggtaa agcgcaagga tcgtcctgga tctttattag atcgattaag
     4261 ccaatttttg tctatggtca ttaaattttc caatatgcgg cgtaaatcgt gcccgcctcg
     4321 cggcaggatc gtttacactt agcgagttct ggaaagtcct gtggataaat cgggaaaatc
     4381 tgtgagaaac agaagatctc ttgcgcagtt taggctatga tccgcggtcc cgatcgtttt
     4441 gcaggatctt gatcgggcat ataaccgcag acagcggttc gtgcgtcacc ctcaagcagg
     4501 gtcttttcga cgtacgtcaa caatcatgaa tgtttcagcc ttagtcatta tcgacttttg
     4561 ttcgagtgga gtccgccgtg tcactttcgc tttggcagca gtgtcttgcc cgattgcagg
     4621 atgagttacc agccacagaa ttcagtatgt ggatacgccc attgcaggcg gaactgagcg
     4681 ataacacgct ggccctgtac gcgccaaacc gttttgtcct cgattgggta cgggacaagt
     4741 accttaataa tatcaatgga ctgctaacca gtttctgcgg agcggatgcc ccacagctgc
     4801 gttttgaagt cggcaccaaa ccggtgacgc aaacgccaca agcggcagtg acgagcaacg
     4861 tcgcggcccc tgcacaggtg gcgcaaacgc agccgcaacg tgctgcgcct tctacgcgct
     4921 caggttggga taacgtcccg gccccggcag aaccgaccta tcgttctaac gtaaacgtca
     4981 aacacacgtt tgataacttc gttgaaggta aatctaacca actggcgcgc gcggcggctc
     5041 gccaggtggc ggataaccct ggcggtgcct ataacccgtt gttcctttat ggcggcacgg
     5101 gtctgggtaa aactcacctg ctgcatgcgg tgggtaacgg cattatggcg cgcaagccga
     5161 atgccaaagt ggtttatatg cactccgagc gctttgttca ggacatggtt aaagccctgc
     5221 aaaacaacgc gatcgaagag tttaaacgct actaccgttc cgtagatgca ctgctgatcg
     5281 acgatattca gttttttgct aataaagaac gatctcagga agagtttttc cataccttca
     5341 acgccctgct ggaaggtaat caacagatca ttctcacctc ggatcgctat ccgaaagaga
     5401 tcaacggcgt tgaggatcgt ttgaaatccc gcttcggttg gggactgact gtggcgatcg
     5461 aaccgccaga gctggaaacc cgtgtggcga tcctgatgaa aaaggccgac gaaaacgaca
     5521 ttcgtttgcc gggcgaagtg gcgttcttta tcgccaagcg tctacgatct aacgtacgtg
     5581 agctggaagg ggcgctgaac cgcgtcattg ccaatgccaa ctttaccgga cgggcgatca
     5641 ccatcgactt cgtgcgtgag gcgctgcgcg acttgctggc attgcaggaa aaactggtca
     5701 ccatcgacaa tattcagaag acggtggcgg agtactacaa gatcaaagtc gcggatctcc
     5761 tttccaagcg tcgatcccgc tcggtggcgc gtccgcgcca gatggcgatg gcgctggcga
     5821 aagagctgac taaccacagt ctgccggaga ttggcgatgc gtttggtggt cgtgaccaca
     5881 cgacggtgct tcatgcctgc cgtaagatcg agcagttgcg tgaagagagc cacgatatca
     5941 aagaagattt ttcaaattta atcagaacat tgtcatcgta aacctatgaa atttaccgta
     6001 gaacgtgagc atttattaaa accgctacaa caggtgagcg gtccgttagg tggtcgtcct
     6061 acgctaccga ttctcggtaa tctgctgtta caggttgctg acggtacgtt gtcgctgacc
     6121 ggtactgatc tcgagatgga aatggtggca cgtgttgcgc tggttcagcc acacgagcca
     6181 ggagcgacga ccgttccggc gcgcaaattc tttgatatct gccgtggtct gcctgaaggc
     6241 gcggaaattg ccgtgcagct ggaaggtgaa cggatgctgg tacgctccgg gcgtagccgt
     6301 ttttcgctgt ctaccctgcc agcggcggat ttcccgaacc tcgatgactg gcagagtgaa
     6361 gtcgaattta ccctgccgca ggcaacgatg aagcgtctga ttgaagcgac ccagttttcg
     6421 atggcgcatc aggacgttcg ctattactta aatggtatgc tgtttgaaac cgaaggtgaa
     6481 gaactgcgca ccgtggcaac cgacggccac cgtctggcgg tctgttcaat gccaattggt
     6541 caatctttgc caagccattc ggtgatcgta ccgcgtaaag gcgtgattga actgatgcgt
     6601 atgctcgacg gcggcgacaa tccgctgcgc gtgcagattg gcagcaacaa tattcgcgcc
     6661 cacgttggcg actttatctt cacctccaaa ctggtggatg gtcgcttccc ggattaccgc
     6721 cgcgttctgc cgaagaatcc ggacaaacat ctggaagctg gctgcgatct gctcaagcag
     6781 gcgtttgccc gtgcggcaat tctctctaac gagaaattcc gcggcgtgcg cctgtatgtc
     6841 agcgaaaacc agctgaaaat caccgccaac aacccggaac aggaagaagc ggaagagatc
     6901 ctcgacgtta cctatagcgg tgcggagatg gaaatcggct tcaacgtcag ctatgtgctg
     6961 gatgttctga acgcgctgaa atgcgaaaac gtccgcatga tgctgaccga ttcggtttcc
     7021 agcgtgcaga ttgaagatgc cgcatcacag tcggctgcct atgttgtcat gccaatgaga
     7081 ctgtaatgtc cctcacccgc ttgttgatcc gcgatttccg caacattgaa accgcggatc
     7141 tcgctttatc tcccggcttt aactttctgg taggtgccaa cggcagtggc aaaaccagcg
     7201 tgctggaagc catctatacg ctcggccatg gtcgggcgtt tcgcagtttg cagattggtc
     7261 gcgtcattcg ccatgagcag gaggcgtttg ttctccacgg gcgattacag ggcgaagagc
     7321 gcgagacagc gattggctta accaaagaca aacagggcga cagcaaagtc cgcatcgacg
     7381 gtacagacgg gcataaggtc gcggaactgg cgcacctgat gccaatgcag ttgataacgc
     7441 cagaagggtt tactttactc aacggcggcc ccaaatacag aagagcattc ctcgactggg
     7501 gatgctttca caacgaaccc ggatttttca ccgcctggag caatctcaag cgattgctca
     7561 agcagcgcaa tgcggcgctg cgccaggtga cacgttacga acagctacgc ccgtgggata
     7621 aagaactgat cccgctggcg gagcaaatca gcacctggcg cgcggagtat agcgccggta
     7681 tcgcggctga tatggccgat acttgtaagc aatttctccc tgagttttct ctgactttct
     7741 ctttccagcg cggctgggag aaagagacag aatatgctga ggtgctggaa cgtaattttg
     7801 aacgcgatcg ccagctaacc tacaccgcgc atggcccgca taaagcggac ttacgcattc
     7861 gcgccgacgg tgcgccggtg gaagatacct tatcgcgtgg gcagcttaag ctgttgatgt
     7921 gcgccttacg tctggcgcaa ggagagttcc tcacccgtga aagcgggcgg cggtgtctct
     7981 acctgataga tgattttgcc tctgagcttg atgatgagcg tcgcgggctg cttgccagcc
     8041 gcttaaaagc gacgcaatca caggtctttg tcagcgcgat cagtgctgaa cacgttatag
     8101 acatgtcgga cgaaaattcg aagatgttta ccgtggaaaa gggtaaaata acggattaac
     8161 ccaagtataa atgagcgaga aacgttgatg tcgaattctt atgactcctc cagtatcaaa
     8221 gtcctgaaag ggctggatgc ggtgcgtaag cgcccgggta tgtatatcgg cgacacggat
     8281 gacggcaccg gtctgcacca catggtattc gaggtggtag ataacgctat cgacgaagcg
     8341 ctcgcgggtc actgtaaaga aattatcgtc accattcacg ccgataactc tgtctctgta
     8401 caggatgacg ggcgcggcat tccgaccggt attcacccgg aagagggcgt atcggcggcg
     8461 gaagtgatca tgaccgttct gcacgcaggc ggtaaatttg acgataactc ctataaagtg
     8521 tccggcggtc tgcacggcgt tggtgtttcg gtagtaaacg ccctgtcgca aaaactggag
     8581 ctggttatcc agcgcgaggg taaaattcac cgtcagatct acgaacacgg tgtaccgcag
     8641 gccccgctgg cggttaccgg cgagactgaa aaaaccggca ccatggtgcg tttctggccc
     8701 agcctcgaaa ccttcaccaa tgtgaccgag ttcgaatatg aaattctggc gaaacgtctg
     8761 cgtgagttgt cgttcctcaa ctccggcgtt tccattcgtc tgcgcgacaa gcgcgacggc
     8821 aaagaagacc acttccacta tgaaggcggc atcaaggcgt tcgttgaata tctgaacaag
     8881 aacaaaacgc cgatccaccc gaatatcttc tacttctcca ctgaaaaaga cggtattggc
     8941 gtcgaagtgg cgttgcagtg gaacgatggc ttccaggaaa acatctactg ctttaccaac
     9001 aacattccgc agcgtgacgg cggtactcac ctggcaggct tccgtgcggc gatgacccgt
     9061 accctgaacg cctacatgga caaagaaggc tacagcaaaa aagccaaagt cagcgccacc
     9121 ggtgacgatg cgcgtgaagg cctgattgcg gtcgtttccg tgaaagtgcc ggacccgaaa
     9181 ttctcctccc agaccaaaga caaactggtt tcttctgagg tgaaatcggc ggttgaacag
     9241 cagatgaacg aactgctggc agaatacctg ctggaaaacc caaccgacgc gaaaatcgtg
     9301 gttggcaaaa tcatcgatgc tgcccgtgcc cgtgaagctg cgcgtcgtgc gcgtgaaatg
     9361 acccgccgta aaggtgcgct cgacttagcg ggcctgccgg gcaaactggc agactgccag
     9421 gaacgcgatc cggcgctttc cgaactgtac ctggtggaag gggactccgc gggcggctct
     9481 gcgaagcagg ggcgtaaccg caagaaccag gcgattctgc cgctgaaggg taaaatcctc
     9541 aacgtcgaga aagcgcgctt cgataagatg ctctcttctc aggaagtggc gacgcttatc
     9601 accgcgcttg gctgtggtat cggtcgtgac gagtacaacc cggacaaact gcgttatcac
     9661 agcatcatca tcatgaccga tgcggacgtc gacggctcgc acattcgtac gctgctgttg
     9721 accttcttct atcgtcagat gccggaaatc gttgaacgcg gtcacgtcta catcgctcag
     9781 ccgccgctgt acaaagtgaa gaaaggcaag caggaacagt acattaaaga cgacgaagcg
     9841 atggatcagt accagatctc tatcgcgctg gatggcgcaa cgctgcacac caacgccagc
     9901 gcaccggcat tggctggcga agcgttagag aaattggtgt ctgagtacaa cgcgacgcag
     9961 aaaatgatca accgcatgga gcgtcgttat ccgaaagcaa tgctgaaaga gcttatctat
    10021 cagccgacgc tgacggaagc cgacctctct gatgagcaga ccgttacccg ctgggtgaac
    10081 gcgctggtca gcgaactgaa cgacaaagaa cagcacggca gccagtggaa gtttgatgtc
    10141 cacaccaatg ccgaacaaaa cctgtttgaa ccgattgttc gcgtgcgtac ccacggtgtg
    10201 gatactgact atccgctgga tcacgagttt atcaccggtg gcgaatatcg tcgtatctgc
    10261 acgctgggtg agaaactgcg tggcttgctg gaagaagatg cgtttatcga acgtggcgag
    10321 cgtcgtcagc cggtagccag cttcgagcag gcgctggact ggctggtgaa agagtcccgt
    10381 cgcggcctct ccatccagcg ttataaaggt ctgggcgaga tgaacccgga acagctgtgg
    10441 gaaaccacca tggacccgga aagccgtcgt atgctgcgcg ttaccgttaa agatgcgatt
    10501 gctgccgacc agttgttcac cacgctgatg ggcgacgccg ttgaaccgcg ccgtgcgttt
    10561 attgaagaga acgccctgaa agcggcgaat atcgatattt aatggcgcta accatgcgag
    10621 cgtgcctgat gcgctacgct tatcaggcct acgaaaatct tgcaatgtat tgaattttca
    10681 tgattttgta ggccggataa ggcgttcacg ccgcatccgg caagaagcta taagaaaagg
    10741 gcggagatca tctccgccct ttttatttct gcaatccgca attatcatcc cgcaaatcta
    10801 tactttttgg gtcaacacgt aacgctttac aggtgattat catggggctt tttgatgaag
    10861 ttgtcggtgc ctttctgaaa ggcgatgcgg ggaaatatca ggctatttta agttgggttg
    10921 aggagcaggg cggcattcag gtgctgctgg aaaaactgca aagtggcggc ttaggggcca
    10981 ttctctcaac ctggctgagt aatcaacagg gcaatcaatc ggttagtggc gagcaactgg
    11041 aatcggcgct cggcacaaat gcggtgtccg atctcgggca aaaacttggc gtggatacca
    11101 gtacagcttc cagtttactg gcagagcaat tgccgaagat tattgatgcg ctctcaccgc
    11161 aaggtgaagt gtcaccacaa gccaataacg atctgctttc cgcaggtatg gaactgctga
    11221 aagggaaact cttccgctaa gcaaatgggg agcatgcaac ccgcgttgca tccccctttt
    11281 ccctcgggtg ttgtactgat ttttgagcgg aatcgcgtta gcatgggtca ggaaccaatc
    11341 tacctgggga actcatggct atcaaactca ttgctatcga tatggatggc acccttctgc
    11401 tgcccgatca caccatttca cccgccgtta aaaatgcgat tgccgcagct cgcgcccgtg
    11461 gcgtgaatgt cgtgctaacg acgggtcgcc cgtatgcagg tgtgcacaac tacctgaaag
    11521 agctgcatat ggaacagccg ggcgactact gcattactta taacggcgcg ctggtacaga
    11581 aggccgctga tggtagcacc gtggcgcaaa ctgctctcag ctatgacgac tatcgtttcc
    11641 tggaaaaact ctctcgcgaa gtcggttctc atttccacgc cctggaccgc accacgctgt
    11701 acaccgccaa ccgtgatatc agctactaca cggtgcatga atccttcgtt gccaccattc
    11761 cgctggtgtt ctgcgaagcg gagaaaatgg accccaatac ccagttcctg aaagtgatga
    11821 tgattgatga acccgccatc ctcgaccagg ctatcgcgcg tattccgcag gaagtgaaag
    11881 agaaatatac cgtgctgaaa agtgcgccgt acttcctcga aatcctcgat aaacgcgtta
    11941 acaaaggtac gggggtgaaa tcactggccg acgtgttagg tattaaaccg gaagaaatca
    12001 tggcgattgg cgatcaggaa aacgatatcg caatgattga atatgcaggc gtcggtgtgg
    12061 cgatggataa cgctattcct tcggtgaaag aagtggcgaa ctttgtcacc aaatccaacc
    12121 ttgaagatgg cgtggcgttt gctattgaga agtatgtgct gaattaatca gtggacgggc
    12181 aaacagcata aacgcgtttg cccgcttact gattattggt ttactgcata tacaaccaga
    12241 aattgcagcg gtgcagtaat gatgtccagc gtaacagtca ctggcagtaa tgctgcggaa
    12301 acaccatccg gaatgcctct gggtgaatag tattcataaa atcgcacttt aaacggatga
    12361 tagaacgcca tcacctttga gtaatccatg tttttatttt tctcatggat tgtgcctttt
    12421 aggttttcca gtgacctggt acagtgttga acaccacagg caaaaccata atgcgttgcc
    12481 agtgcctttt cttcttcgtt attccagtag tagtaaagcg atattgtgcc ggtaaatttc
    12541 tttttccccg catttaaaac aaatcttgcg tcatcggcaa cctgaatatt gtgccggtta
    12601 agtgctggat cattgagcat cttaacgatg tcatcggcac catcagtgat aaggtaatcc
    12661 agcgattccc ctacaaaaac atagcctttt gttccattac tatcttttgc ctgcgatagc
    12721 ccagtgatgg tgtctgactg cgattgatac tttcctgaac taccctttgc ggtatgggaa
    12781 accagagcat acgagataca gcctgaaagc atcagcgcca gtgggaataa accggcagcc
    12841 ttaaaaaatc ccttaaaatt caacttcatg attaatcctt taaacgagta gctaagccgt
    12901 tgaaagcata ctcttctttc cctcctgttt agcgcctgat atcccttttc agatttctgc
    12961 ccgacgcatg tcattttttt atgcattgtt ctttttgtga tctaaattgt agtacaacaa
    13021 tataagtttg tactacatta cgcacacggc aaacgcgaac gtcatcacgc tggtactaca
    13081 aagttgccgc gttatgcatc gatcggggta aagtagagaa gaacatacag agcacaagga
    13141 ctctccatga ctctcaataa aaccgatcgc attgtcatta cgctgggtaa acagatcgtt
    13201 cacggcaaat acgtacctgg ctcgccactt ccggctgagg cggagctctg tgaagagttt
    13261 gcaacctcgc gcaacatcat ccgtgaggtg ttccgttcgt tgatggcgaa gcggctgatt
    13321 gaaatgaaac gttatcgcgg cgcgtttgtg gcaccgcgta accagtggaa ttacctcgac
    13381 actgacgtac tgcaatgggt gctggaaaac gactacgacc cacggcttat cagtgccatg
    13441 agcgaagtgc gaaatctggt ggaaccggcg attgcccgtt gggcagcaga gcgcgcgacc
    13501 tccagcgatc tggcgcagat tgaatcggcg ctgaacgaga tgattgccaa caatcaggac
    13561 cgcgaagcgt ttaacgaagc ggatattcgc taccacgagg cggtgctgca gtcggtgcat
    13621 aacccggtgt tacagcaact tagcattgcg atcagttcgc tgcagcgggc ggtttttgaa
    13681 cgaacctgga tgggcgatga ggccaacatg ccgcaaacgc tccaggaaca taaggcgctg
    13741 ttcgatgcga tacgacatca ggacggcgat gcggcagagc aggcggcgct taccatgatc
    13801 gccagctcga cacgaaggtt aaaggaaatc acatgacagc tcgctacatc gcaattgact
    13861 ggggatcgac caatctgcgt gcctggcttt atcagggcga ccactgcctg gacagcaggc
    13921 aatcagaagc aggcgtcacg cgcctgaacg gaaaatctcc ggctgcggtg ttagcagaag
    13981 tcacgaccga ctggcgtgaa gagaatacgc cagtggtgat ggcaggaatg gtcggcagca
    14041 atgtcggctg gaaagttgca ccgtatttat ctgttcctgc ccgtttttcg tctattggcg
    14101 atcaattaac gtcggttggc gacaatatct ggattattcc cggattatgc gtctctcatg
    14161 acgataacca caatgtgatg cgcggcgaag aaacacaatt gatcggcgcg cgcactctgg
    14221 ctccttcctc tctttatgtc atgcccggaa cccattgcaa atgggtgcag gccgatagcc
    14281 agcaaatcaa cgattttcgc accgtgatga ccggtgaatt acatcattta ctgttaaatc
    14341 actcattgat tggcgcaggt ttgccgccgc aggaaaactc tgccgatgcc ttcgcggctg
    14401 gccttgagcg tggccttaat gcgcccgcca tattgccgca gctttttgaa gttcgcgcct
    14461 cgcatgtgct gggaacactt ccccgcgaac aggtcagcga atttctctct ggtttgttga
    14521 ttggcgcaga ggtcgccagt atgcgcgact atgtggccca tcaacacgcc atcacccttg
    14581 tcgccggaac atcgctgacc gcgcgctacc agcaagcctt tcaggcgatg ggttgcgacg
    14641 tgacagcggt agcaggcgac acggcatttc aggctggtat aaggagcatc gctcatgcag
    14701 tggcaaacta aactcccgct gatcgccatt ttgcgcggca ttaagcccga cgaggcgctg
    14761 gcgcatgttg gcgcggtgat tgacgccggg ttcgacgcgg ttgaaatccc gctgaattcc
    14821 ccacaatggg agcaaagcat tcccgccatc gttgatgcgt atggcgacaa ggcgttgatt
    14881 ggcgcaggta cggtactgaa acctgaacag gtcgatgcgc tcgccaggat gggctgtcag
    14941 ctcatcgtta cgcccaatat ccatagtgaa gtgatccgcc gtgcggtggg ctacggcatg
    15001 accgtctgcc ccggctgcgc gacggcgacc gaagccttta ccgcgctcga agcgggcgcg
    15061 caggcgctga aaatatttcc gtcatcggct tttggtccgc aatacatcaa agcgttaaaa
    15121 gcggtattgc catcggacat cgcagtcttt gccgttggcg gcgtgacgcc agaaaacctg
    15181 gcgcagtgga tagacgcagg ttgtgctggg gcgggcttag gcagcgatct ctatcgcgcc
    15241 gggcagtccg tagaacgcac cgcgcagcag gcagcagcat ttgttaaggc gtatcgagag
    15301 gcagtgcaat gaaaatcacc aaaattacca cgtatcgttt acctccccgc tggatgttcc
    15361 tgaaaattga aaccgatgaa ggcgtggtcg gttggggcga gcccgtgatt gaaggccgcg
    15421 cccgtacggt ggaagcggca gttcacgagc tgggtgacta tttgattggt caggatcctt
    15481 cgcgcatcaa tgacttatgg caagtgatgt atcgcgccgg attttatcgt ggcggtccaa
    15541 tcctgatgag cgccattgcc gggatcgacc aggcgttatg ggatatcaaa ggtaaagtgc
    15601 tgaatgcgcc ggtctggcaa ctgatgggcg gcctggttcg cgacaaaatt aaagcctaca
    15661 gttgggtcgg cggcgatcgt ccggcggatg tgatcgacgg tattaaaacc ctgcgcgaaa
    15721 tcggcttcga taccttcaaa ctgaacggtt gtgaagaact ggggctaatt gataactccc
    15781 gcgcggtaga tgcggcagtc aacaccgtgg cacaaattcg tgaagctttt ggcaatcaga
    15841 ttgagtttgg tcttgatttc cacggtcgcg tcagcgcgcc aatggcgaaa gtgctgatta
    15901 aagaactgga gccgtatcgc ccgctgttta ttgaagagcc ggtgctggcg gaacaagccg
    15961 aatactaccc gaaattggcg gcacaaacgc atattccact ggcggcgggt gagcgcatgt
    16021 tctcacgttt cgattttaaa cgcgtgctgg aggcaggcgg tatttcgatt ctacaaccgg
    16081 atctctccca tgcaggcggt attaccgaat gctacaaaat tgccggaatg gcagaagcct
    16141 atgacgtgac ccttgcgccg cactgtccgc tcggaccgat tgcactggcg gcttgtctgc
    16201 atatcgactt tgtttcctat aacgcggtac ttcaggaaca aagtatggga attcattaca
    16261 acaaaggcgc ggagttactc gactttgtga aaaacaaaga agacttcagc atggttggcg
    16321 gcttctttaa accgctaacg aaaccgggct taggcgtgga aatcgacgaa gctaaagtta
    16381 ttgagttcag taaaaatgcc ccggactggc gtaatccgct ctggcgtcat gtagataaca
    16441 gcgtagcaga gtggtaattc ctgccacgta agcccctcat cgggcactaa aacagcaata
    16501 caaaaatata accctctgta aattacaggg catggtgagc ggcttcgcta tgcccaaaat
    16561 ctggagaaag ctcgctatgg atattcccgt taatgcagca aagccggggc gtcggcgtta
    16621 tctgacgctg gtgatgatct ttattacggt ggtcatttgt tatgttgacc gcgctaacct
    16681 ggccgtggct tccgcccata ttcaggaaga gttcggcatt acgaaagcgg aaatgggcta
    16741 tgtattttcg gccttcgcct ggctttatac gctatgccag atccccggcg gttggttttt
    16801 agatcgcgta ggttctcgcg tgacttatta tattgcgata tttggttggt cagtggcgac
    16861 tttattccag ggctttgcca cgggattaat gtcattaatt ggtctgcgtg cgataaccgg
    16921 tattttcgaa gcgcccgctt tcccgaccaa taaccggatg gtgaccagct ggttcccgga
    16981 acatgaacgc gcttccgccg ttggttttta tacgtctggt cagtttgtcg gtctggcgtt
    17041 tttgactccg ctgctgatct ggattcagga gatgttgagc tggcactggg tgttcattgt
    17101 cactggtggt atcggcatta tctggtcgct gatttggttt aaggtttatc agccgccgcg
    17161 cctgaccaaa ggtatcagca aagctgaact ggattacatt cgtgatggcg gcggtctggt
    17221 ggatggtgat gcgccggtga agaaagaggc gcgtcagccg ttaacagcca aagactggaa
    17281 actggtgttc catcgtaaac tgatcggcgt ttatcttggg caatttgcgg tggcttctac
    17341 actgtggttt ttcttaacct ggttcccgaa ctatttaacc caggaaaaag gaatcacggc
    17401 gctgaaagca ggctttatga ccacggtgcc attcctcgcg gcgtttgtcg gcgtcctgct
    17461 ctctggctgg gtagcggatc tgctggtacg taagggcttt tcactgggct ttgcgcgtaa
    17521 aacgccgatt atctgcggct tgctgatctc cacctgcatt atgggcgcta actacactaa
    17581 cgatccgatg atgattatgt gcctgatggc gctggcattc ttcggtaacg gttttgcttc
    17641 gattacctgg tcgctggttt cttctctggc accgatgcgc ctgattggtt taaccggcgg
    17701 cgtgtttaac ttcgccggtg gtctgggcgg catcaccgtt ccgctggtgg tggggtacct
    17761 ggcgcagggt tacggtttcg cacctgcact ggtttatatc tccgccgtcg cgttgattgg
    17821 cgcgctctct tatatcctgc tggtgggcga tgtgaagcgc gttggctaat ccttcaactg
    17881 tggaatatgt gccacaccac tgcgcataat ccactttctt aaagccggtg cggtcaggca
    17941 tcggcttttt actatcttgc cgaagagttt taaacgcagt ttgcgggtgg cgcgccagta
    18001 agcggtattg agcttctctg gctgcttcag taacaccgaa cgcagaatgt ctgcgctatc
    18061 cagcgcatag ctaatcccct ccagcgagct ggcgctgata aatcccgccg cttcaccaat
    18121 cagaaaggcg ttgtccttac cgcagacaaa atcctgccag cgtgagggaa acagcaccgt
    18181 gcatttttcg cttttcaccg ccttaccaaa ctggaactga aaggcgctca ttttctcttt
    18241 cagcgtcgtg aaacgcgtct gaccgtcttt cattggataa gcaccgccaa agataaaata
    18301 accgtctttg ctgatactcc atgaataaca gttagttatc gaattatcaa agatgcagga
    18361 gtagaacggc accggatgtt tctccgcgaa ccactgctgg atagcgacat atttacggat
    18421 ttgatgatcc gggtagagat gtcgccgcac catcgagttt gcgccatcgg caccgaccag
    18481 atagcgggcg gtaatatgtt gctcccagcc gtctgcacga aaaatgacat gccatttatc
    18541 atcctcacgc cagatttttc ggcacaggct atcgtgataa acctcaacgc tggtggggat
    18601 cagcgatttc atccacaagt cgaaagcatg gcgattaata ttgatatagc ttcgctggta
    18661 gttacgcgtg agcgatgcgg cgacgtcgac agttttgacg ctgaaaatct gtggattggc
    18721 gatcacatcg acaggaagcg tcagtccatc gcgaataaaa gatcgctgcg cgtccggtgc
    18781 cagcagaccg ccgcaaggtt tgctgaaacc ttcagtacca cactggtgct ttttatccag
    18841 cgcgatcacc tgcattttgc ctgctaattt tcgcgccaac gccgatcctg ccgggccgag
    18901 gccaataatc gccacgtcga aatgttccat cttttcactc acattcatca cgatggcaaa
    18961 agactattgt ctgaaagtga agtccttgtg aagtatgatg gcgggacccg ttttgtatca
    19021 ggaccgcgcc atgaaacaga taacctttgc tccccgtaat cacctgctca ccaataccaa
    19081 tacctggacg cccgacagcc agtggctggt atttgacgtg cgtccttctg gggcgtcgtt
    19141 taccggcgag accattgagc gtgtgaatat ccataccggc gaggtcgagg ttatctatcg
    19201 cgcgtcacag ggcgcacacg tcggcgtggt gaccgttcat ccgaagtcag agaaatatgt
    19261 tttcatccat ggcccggaaa atcctgatga aacatggcat tacgatttcc atcatcgacg
    19321 cggagtcatt gctgaaggcg gcaaggtgag caatctcgat gcaatggata ttaccgcgcc
    19381 gtatacgcca ggagcgctgc gcggcggcag ccatgtgcat gtctttagcc cgaacggtga
    19441 aagggtgagc tttacctata acgaccatgt aatgcatgaa ctcgatccgg cgctggattt
    19501 gcgaaacgtc ggtgttgctg cgccgtttgg cctggtcaac gtacaaaagc agcatccgcg
    19561 tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa accacgccca cgccgcagcc
    19621 tggcagcgat gaaatcaatc gtgcttatga agaaggatgg gtaggaaatc acgcgctggc
    19681 gtttattggc gacacacttt cgccaaaggg cgagaaagtg ccggagctgt ttatcgttga
    19741 gttaccgcaa gatgaagctg gctggaaagc ggcaggtgat gcgccgttaa gtggaacgga
    19801 aaccaccctg cccgcgccac cgcgcggcgt cgtgcagcga cgtttaacct ttacccacca
    19861 tcgggcttat ccggggttag tcaacgtccc gcgccactgg gtgcgctgta atccgcaggg
    19921 tacgcaaatc gcgtttttaa tgcgtgatga taacggcatt gtgcaactgt ggcttatctc
    19981 gccacagggc ggcgagccgc gccagttaac ccataacaaa acggatattc agtctgcatt
    20041 taactggcat ccgtcaggag aatggttggg ctttgtgctg gataatcgaa ttgcttgtgc
    20101 ccatgcgcaa agcggcgagg ttgagtattt aaccgaaaac cacgccaatc caccttctgc
    20161 ggacgccgtg gtcttctcgc cggatggtca gtggctggcg tggatggaag gcggccagct
    20221 gtggatcacc gaaactgatc gctaaatcag ttatcaggca ttctggccgg tggaataacc
    20281 gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgc gtgagcgcac cgaactgtct
    20341 ttgcggaaca catcccacgg cagtaaaagc gtgtccatca cagcggtaaa cggcatatcg
    20401 agaatcgcca gcgacttagt gccccagttg gtttcatcgt cgccaatcat ggttgcgctg
    20461 gcgcgggtgc cgggatacgt tccttctttc cctccggtat gggacatcac gctggagcat
    20521 cctccgagta atgtcattcc gctgcatatc atgaacgcta acaacacatt tcttatcata
    20581 ataaattcat ctgttgatcg tgggtgttgg cctgatgagt tatagcgatc ccttgctgaa
    20641 aataacatca tcattacgtc gcactgtggc ggctatcgca ctttaacgtt tcgtgctgcc
    20701 ccctcagtct atgcaataga ccataaactg caaaaaaaag tccgctgata aggcttgaaa
    20761 agttcatttc cagacccatt tttacatcgt agccgatgag gacgcgcctg atgggtgttc
    20821 tggctacctg acctgtccat tgtggaaggt cttacattct cgctgatttc aggagctatt
    20881 gattatgcgt aactttgatt tatccccgct ttaccgttct gctattggat ttgaccgttt
    20941 gtttaaccac ttagaaaaca accagagcca gagtaatggc ggctaccctc cgtataacgt
    21001 tgaactggta gacgaaaacc attaccgcat tgctatcgct gtggctggtt ttgctgagag
    21061 cgaactggaa attaccgccc aggataatct gctggtggtg aaaggtgctc acgccgacga
    21121 acaaaaagag cgcacctatc tgtaccaggg catcgctgaa cgcaactttg aacgcaaatt
    21181 ccagttagct gagaacattc atgttcgtgg cgctaacctg gtaaatggtt tgctgtatat
    21241 cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc cgtatcgaaa tcaactaatt
    21301 ccttaaggcc gcctggcgcg gcctgacatc tccatgctcg ccgtcaggga gcatatgcga
    21361 atcttcggat ttgcaggtac ttactcgctt cttagaagga gaaatgacta tgcgtaactt
    21421 cgatttatcc ccactgatgc gtcaatggat cggttttgac aaactggcca acgcactgca
    21481 aaacgccggt gaaagccaga gcttcccgcc gtacaacatt gagaaaagcg acgataacca
    21541 ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa gatttagaga ttcaactgga
    21601 aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca aaagaagaga aaaaatggct
    21661 gcatcaaggg cttatgaatc agccatttag cctgagcttt acgctggctg aaaatatgga
    21721 agtctctggc gcaaccttcg taaacggttt actgcatatt gatttaattc gtaatgagcc
    21781 tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt cccgcgttaa atagctaact
    21841 agctattctc tttgccccgc cattctggtg gggctttttt tgtctttctt cttcaacaat
    21901 gcgttaaatt tcgcctgttt aagatatttc ggcacgttct ttcccgtgat aatgtgcgct
    21961 ataaggcaaa tctgcttcac cgattcagcc ataatccgta gcaattaaaa atgttatctc
    22021 aagagaaatg gacgatgagt gatatagcat taacggtcag tattctggct ttggtggcag
    22081 tcgtcggttt gtttatcggc aatgtcaaat ttcgcggcat aggattaggt attggcggcg
    22141 tgctgtttgg tgggatcatc gtcggccatt ttgtttctca ggcggggatg acattaagta
    22201 gcgatatgct gcatgttatt caggaatttg gcctgatcct gttcgtttat actatcggga
    22261 ttcaggtagg gccgggcttc tttgcctcat tgcgcgtctc cggattacgc ctcaacctgt
    22321 ttgctgttct gatcgtcatc atcggtggtc tggttaccgc catcctgcat aaactgtttg
    22381 atattccact gccggtagtg ctggggattt tctccggtgc ggttaccaat acgccagcgc
    22441 tgggggcagg gcagcagatt ttgcgcgacc tgggtacacc aatggaaatg gtcgatcaga
    22501 tggggatgag ttacgcgatg gcgtatccat tcggcatttg cgggattttg ttcaccatgt
    22561 ggatgttgcg ggttattttc cgcgtcaatg tcgagacaga agctcagcag cacgagtctt
    22621 cacgcaccaa tggcggcgcg ctgatcaaga ctatcaatat tcgcgttgag aaccctaacc
    22681 tgcatgattt agccattaaa gatgtaccga ttctcaacgg cgacaaaatt atctgctcgc
    22741 gtctgaaacg cgaagaaacc ctaaaagttc cttcgccaga taccattatt caactgggcg
    22801 atttgctgca tctggtgggt cagccagcgg atttacataa tgcgcaactg gtgattggtc
    22861 aggaggtcga tacttcgctg tccacgaaag gcactgattt gcgcgtcgag cgtgtggtgg
    22921 tcaccaatga aaacgtgctc ggaaaacgta ttcgcgacct gcactttaaa gaacgctatg
    22981 acgttgttat ctcgcgcctg aaccgtgccg gggtcgaact ggtcgccagt ggcgatatca
    23041 gcctgcagtt cggcgatatc ctcaacctgg tggggcgtcc gtccgcaatt gatgccgttg
    23101 ccaatgtgct ggggaatgcg cagcaaaaac tgcaacaggt tcagatgctg ccagtgttta
    23161 ttggcatcgg gcttggcgta ttgttaggtt ctattcccgt ctttgtgcca ggattcccgg
    23221 ccgcgttgaa actggggctg gcgggcggtc cgctgattat ggcgttgatc ctcgggcgta
    23281 tcggcagtat cggcaagctg tactggttta tgccgccaag tgccaacctc gcgctgcggg
    23341 agctggggat cgtgctgttc ctctcggtcg ttggtctgaa atctggtggg gattttgtga
    23401 ataccctggt caatggcgaa gggctaagct ggattggtta tggtgccctg atcaccgccg
    23461 ttccgctgat tactgttggc attctggcgc ggatgttagc caaaatgaat tacctgacca
    23521 tgtgcgggat gctggcaggt tccatgaccg atcctccggc gctggcgttt gctaataatc
    23581 ttcatccaac cagtggtgcg gcggcgctct cttacgccac tgtctatccg ttagtgatgt
    23641 ttctgcgaat tatcaccccc caattactgg cggtgctctt ctggagtatc ggttaactct
    23701 tttccgggtg gagtcgccgt aaatggtact ccacctggta ttcgctggca tttctgaaca
    23761 tcactgaata attcaaaaac tccccgctct cgctatatga caacgaggtg atccgcagca
    23821 gtggcgtgtg ttccgctacc ttcatttgcc gcgccagcag tctatcggcg agcaccggcg
    23881 tcaggctttc ataattaccg ccaatcaaaa tcccgcactc ttgttcaata tattcaaact
    23941 tcgacccttc cagatgttgc agcgaaaggt tacggaacag ttttaccggc atatagctgt
    24001 cttccagcat cagcggtttc ccttccacaa aacgaacgcg acgggagaag tagatctgct
    24061 cgttgatttg aatccgtaac tggctggcaa tgaccggagg cgcaggcatg atttcaaaaa
    24121 tcagcacctg gctggtgacc gtttttccct gccgttttaa cacctccacc agcccggtca
    24181 ggctggcggt ttgatgcagt acatctttgc gcaccaggta agtgccgctg ccgtggcggc
    24241 ggaccaccaa tccccacgct accagcaggt caatggcttt acggatggtc atccgcgata
    24301 ccgcaaattc ttccgccaac tttttttcac cgggaagaag gctatttagc gtgaaatctg
    24361 cggaattaag tcgaattctt aaccgctccg caatgctttt gtagatcatc tctagatctc
    24421 ttttttgcct gcaagcccgg gtaatgcgcc cacaagttct gcttaatcga ttgaaaaaac
    24481 atcttttttt taaagatgtg ttcgatgctg tgtacctgtg ctcacagatg tctacttttt
    24541 cgcgaaaacg tagatctcta ccgcccaacg aaaagcatga aagcgatcac gaatcccatt
    24601 gggtcgtcat gttctgccga tcgcatcttc ctatcctcgc tccaggcctg ccgcataacc
    24661 aatcaggctt cctacttaca gaattgagaa aagaggatgt ggaaatgctc agtcaaattc
    24721 aacgctttgg cggcgcgatg ttcacgccag tgctgctgtt tcccttcgcc gggattgtgg
    24781 tgggtcttgc catcttgctg caaaacccga tgtttgtcgg ggaatcactg accgatccga
    24841 acagtttatt cgcgcaaatc gtacacatta ttgaagaggg cggttggacg gtattccgta
    24901 atatgccgct gatttttgct gtcggtttac ccattggcct tgctaagcaa gcgcagggac
    24961 gtgcttgtct ggcggtgatg gtgagtttcc tgacctggaa ctatttcatc aatgcgatgg
    25021 gaatgacctg gggaagctac ttcggcgtcg atttcactca ggacgcggtg gcaggtagcg
    25081 gtctgacaat gatggctggg attaaaaccc tcgataccag cattatcggc gcaattatca
    25141 tttccggcat tgtgacggcg ctgcataacc gtctgttcga taaaaaactg ccggtgtttc
    25201 tcggcatttt ccaggggacg tcttatgtgg tgattatcgc cttcctggtg atgatcccct
    25261 gtgcctggct gacattgctc ggctggccaa aagtacaaat ggggattgaa tctctgcaag
    25321 cgttcctgcg ttcggcgggt gcgcttgggg tgtgggttta caccttcctc gaacgtattc
    25381 tgatcccaac cggtttacac cacttcatct acggaccgtt tatctttggt ccggcagctg
    25441 ttgaaggcgg cattcagatg tactgggcgc agcatctgca agagttcagt ctgagcgccg
    25501 agccgctgaa atcgttgttc ccggaaggcg gttttgccct gcacggtaac tcaaaaatct
    25561 ttggtgccgt gggcatttct ttagcgatgt acttcactgc cgcaccggaa aatcgggtaa
    25621 aagtggcggg cttgctgatt cccgcaacct taaccgccat gctggtggga attaccgaac
    25681 cgctggaatt taccttcctg ttcatttcac cgttgctgtt tgcggtacac gccgtgctgg
    25741 cggcctcaat gtcgaccgta atgtatctct ttggtgtggt gggcaacatg ggcggaggtc
    25801 tgattgacca ggttttaccg caaaactgga tcccgatgtt cagcaaccac gcggatatga
    25861 tgctgaccca aatcgccatt gggttgtgct ttaccctgct gtacttcgtg gttttccgca
    25921 cactgattct gcagttcaac atgtgcacgc cgggacgtga agatgcggaa gtgaaactct
    25981 actcaaaagc cgaatacaaa gcctcgcgag gccaaaccac cgcggcagag ccaaaaaaag
    26041 agctggatca ggctgccggt atcctgcaag ccctgggcgg ggtcggcaat atctccagca
    26101 ttaacaattg cgcgacgcgt ttacgcattg cactgcatga catgtcacaa acgctggatg
    26161 acgaagcctt taaaaagctg ggagcgcacg gcgtcttccg tagtggcgat gccattcagg
    26221 tgatcattgg tctgcatgta tcccagctgc gtgaacagct cgatagctta attaattctc
    26281 atcaatcagc agaaaatgtt gccattacgg aggcagtata atgaccaaat tctcagtggt
    26341 tgtcgcaggc ggtggaagca cctttacgcc aggcatcgtg ttgatgctcc tggcgaatca
    26401 ggagcgtttc ccgcttcgtg cactgaaatt ttatgataac gatggcgcgc ggcaggaagt
    26461 gattgccgaa gcctgtaaag tcatccttaa agagaaagcg ccggacattg cgtttagtta
    26521 caccaccgat cctgaagtgg cgttcagcga cgttgatttt gtcatggcgc acatccgcgt
    26581 cggcaagtac ccgatgcgcg aactggatga aaaaatccca ctgcgccacg gtgttgttgg
    26641 tcaggaaact tgcggacccg gcggaatagc gtacggcatg cgttccattg gcggcgtcct
    26701 ggaactggtg gattatatgg aaaaatattc accaaatgcc tggatgctca actactccaa
    26761 cccggcagcc attgtcgcag aagccacgcg tcgtctgcgc ccgaatgcga aaatcctcaa
    26821 catctgtgac atgccaatcg gtattgaaag ccggatggcg caaattgttg ggctgcaaga
    26881 tcgcaaacag atgcgcgtgc gctactacgg cctgaaccac tggtggtcgg cgatatcccg
    26941 cagtttcaga aagggttaat gagtcagcaa gtggcggtgg aaaaactggt cgtggacgcc
    27001 tgggaacagc gttcatatca gcacctgtgg caggcgatta cgttgtcgaa aacggtaccg
    27061 agcgcctcgg tcgccaaagc tattctggat gagttgctgg aggccaacaa agcgtactgg
    27121 ccagagttac gttaattgtg tggactggca tccgttgggt gctggtccgt attctttagc
    27181 acggctgacg gcgcataacc aaatttatgt ttaaaggctt tgctgaaatg aaaggagtcg
    27241 aagaagttga gcgtatcagc gacttcgcca accgagtttc cttgttgctt taatagcgat
    27301 agcgccagat ccagacgcgc atcgaggtaa tattctttcg gcgtcttgcc ggtatagcgt
    27361 aaaaacagac gacgcaacca ggcttcgcta caggggatcg tggcagccat atcagctacg
    27421 ctccagcgtt gttgcagact ggcatgtaaa gtggcaatta atttttcaat ttgccgccgt
    27481 tgtggatctt ttttaccatc tgcgtacata agacaaatcc actgataaat aattttcgtc
    27541 agaaaagcga ctgccagatt attttttatt gcttctggtg aagttattaa ctcagcaact
    27601 tcggtgagtt cctgattata aatttcgcca ttataaataa cgctttgctg accaacggga
    27661 atatccatca tactggtggg ggtaaattcc atccagtact gttcccagac taaaccttca
    27721 cagtgataag agtgaatatc cattggcgtt aagaatataa tacagttgcc atgcagtgtt
    27781 atttgttcgc cattctttaa aagaattttc ccacatcctt gtacggtata caccgcaacc
    27841 catgagttgg cgataagcgg tttcttcttg tcccgcggcc agatcacccg gtaatgctcg
    27901 tcggcgtagg tatgccagag cgagatcaga gaaattccgc tggagatcac attttcgtta
    27961 atcaataagg gaatattgta cggagtttcg ttttttacat ctgaactttg caattttcca
    28021 ttcattttaa tatccctccg gcgaattatg ctcacaacct gaacttaaca gaaaacaatc
    28081 cgttcaggga tacgacgttt gtattttaag aatctgactg cctgacccga cgcattttac
    28141 ctctccctat attcatgcgt ccgggacata aacataaata agggctatga gatgaattcg
    28201 ttacaaatct tgagttttgt cggttttacg ctgctggtgg cggtgatcac ctggtggaag
    28261 gtccgcaaaa cagataccgg atcgcaacaa ggctattttc ttgccggacg ttcactaaaa
    28321 gcgccggtta ttgccgcttc gttaatgcta accaaccttt ccacggaaca actggtcggc
    28381 ctttccgggc aggcctacaa aagcggcatg tcggtgatgg gctgggaagt gacttcagcg
    28441 gtgacgctga tcttcctcgc gctaatcttt ttaccgcgct atctgaagcg cggcattgcc
    28501 accatccccg attttctgga ggaacgttat gataaaacga cgcgtattat catcgacttc
    28561 tgcttcctca ttgccaccgg cgtctgcttt ctgccgattg ttctctactc cggcgcgttg
    28621 gcgctcaaca gcctgtttca cgtcggggaa tcgctacaga tttctcacgg tgcggctatc
    28681 tggctactgg taattttgct tggtctggcg ggaattttgt atgcggtgat cggcggactg
    28741 cgcgcaatgg cagtggcgga ctccatcaac ggtattgggc tggttatcgg cgggttgatg
    28801 gtgccggtat ttggcctgat cgcgatgggc aagggcagct ttatgcaggg cattgagcaa
    28861 ctcaccaccg ttcacgccga gaaattaaac tcaatcggta gcccgaccga tcccttgccg
    28921 attggcgcgg catttaccgg tttgattctg gtgaacacct tttactggtg tacaaatcag
    28981 ggcatcgtgc aacgcacgct ggcgtcaaaa agcctggcgg aagggcaaaa gggggcgctg
    29041 ttaacggcgg tgctgaaaat gctcgacccg ctggtactgg tgctgccagg gttgattgcg
    29101 tttcatctgt atcaggattt accgaaagcc gacatggcct acccgacgct ggtcaataac
    29161 gttctgccag tgccaatggt gggtttcttc ggcgcggtgt tatttggtgc ggtgatcagt
    29221 accttcaacg gctttctgaa tagcgccagt acgttattca gtatgggtat ttaccgtcgc
    29281 atcattaacc agaatgccga gccgcagcag ctggtcaccg tcgggcgcaa atttggtttc
    29341 tttatcgcta tcgtttcggt gctggtcgcg ccgtggatcg ccaacgcgcc gcaggggctg
    29401 tatagctgga tgaaacagct caacggcatt tacaacgtgc cgctggttac catcatcatt
    29461 atgggctttt tcttcccgcg catcccggcg ctggcggcaa aagtggcgat ggggattggc
    29521 ataatcagct acatcaccat caactatctg gtgaagttcg acttccattt cctctatgtg
    29581 ctggcctgta cgttctgcat caacgtggtc gtgatgctgg tgatcggttt tatcaaaccg
    29641 cgcgccacgc cgttcacctt caaagatgcg tttgcggtgg acatgaaacc gtggagaaac
    29701 gtcaagatcg cgtcaattgg catcctgttc gcgatgattg gcgtctatgc cgggctggct
    29761 gaattcggcg gctacggtac gcgctggtta gcgatgatca gttatttcat tgccgccgta
    29821 gtgattgtct acctgatttt tgacagctgg cggcatcgtc acgacccagc cgtaaccttt
    29881 actcccgacg cgaaggatag cctatgaaac gccccaattt tctgttcatc atgaccgata
    29941 cccaggccac caatatggtc ggttgctata gcggtaaacc gctgaatacg caaaatattg
    30001 atagtctggc ggcggaaggt attcgcttta attccgccta cacctgttca ccggtttgta
    30061 cgcctgcacg cgccggacta tttaccggta tctacgctaa ccagtccggc ccgtggacca
    30121 acaacgtcgc gccaggcaaa aacatctcca ctatggggcg ctactttaag gatgccggct
    30181 atcacacctg ttacatcggc aaatggcatc tcgacggtca tgactatttc ggcactggcg
    30241 agtgtccgcc ggagtgggac gctgattact ggttcgatgg ggcgaactac cttagcgaac
    30301 tgacggaaaa agagatcagc ctgtggcgca atggcctaaa cagcgttgag gatttacagg
    30361 cgaaccatat cgacgaaacc ttcacctggg cgcaccgcat cagcaatcgg gcggtagatt
    30421 ttctgcaaca gcccgcgcgc gccgaggaac ccttcctgat ggtggtttcg tatgatgagc
    30481 cgcatcaccc gttcacctgt ccggtggagt atttagagaa atacgctgat ttttactacg
    30541 atctgggcga gaaagctcag gatgacctgg cgaacaaacc ggaacatcac cgcttatggg
    30601 cgcaggcgat gccatcgcca gtcggtgatg acgggcttta tcaccatccg ctctattttg
    30661 cctgtaatga ctttgttgat gaccaaatcg gacgggtcat caatgcctta acgccagagc
    30721 aacgtgaaaa tacgtgggtc atttatactt ccgatcacgg cgaaatgatg ggcacacata
    30781 agctgatcag taaaggagcg gcgatgtatg acgacatcac ccgtattccg ctgatcatcc
    30841 gttcgccgca aggggagcgg cgacaggtcg atacgccagt cagtcatatc gatttactgc
    30901 cgacaatgat ggcgctggca gatattgaaa aaccagagat tctgccgggg gaaaatatcc
    30961 ttgccgtgaa agagccacgc ggtgtaatgg tggaatttaa ccgctacgag attgagcatg
    31021 acagctttgg cggttttatt ccggtgcgtt gctgggtgac ggatgacttt aaactggtac
    31081 tcaacctctt caccagtgat gaactttacg atcgccgtaa tgacccaaat gaaatgcata
    31141 acctgatcga tgatatccgt tttgcagacg ttcgcagcaa aatgcatgac gccttattgg
    31201 attacatgga caaaattcgc gatccgttcc gcagttacca atggagcctg cgtccgtggc
    31261 gtaaagatgc actgccgcgc tggatggggg catttcgtcc acgcccacaa gatggctatt
    31321 cgccggtggt acgtgactat gacaccggcc taccgacgca aggagtgaaa gtggaagaga
    31381 aaaaacagaa gttctgacga taatgcccgc tgatgaacac ccggcgggca ttattcaggc
    31441 tgcttaaacc agtgaaccgc cggtcaggaa ggaaaaaatc aggaatatca gcaaaatgct
    31501 gcacacgatg ccgataccaa aaagcaaggt atggaaagcc agcgtcccgc cgccaaaaac
    31561 cagcgcgccg cgaatgcccc agcgattttc cttacgcata ccgccaataa atgcgcaaag
    31621 tagcgcagca atggccagtg cgataccgct ttgatcgagg attttatcga catctacgtc
    31681 ctcttttttc tccacggtgg taattttttc gccttttatt ccggcgagaa gacctctttt
    31741 aatttcagcc gttttgtccg ccacgatgct ttcaaacgtt ggcggtggtg cggaaaaagg
    31801 gccgaatgaa aagtggataa tccccatcat taaagcgatg gcaccgaaca gcatgcccaa
    31861 tgaactgatt ttattttgag ctatgatacc catctgacct ccttgtagcg gatggcgaaa
    31921 gcatatcagg aaacgggcgg acgaaattct taaaaaaaaa ccgctaaagg tagagcgtta
    31981 gtgaaattgc aaaggagcaa caatgaaaat ttcccgcctt ggagaagcgc ccgattaccg
    32041 cttctcgctg gctaatgagc gtacttttct ggcgtggatc cgtaccgcac taggatttct
    32101 ggcggcgggg gttgggcttg atcaacttgc gccagatttc gccacgcccg tcattcgtga
    32161 actgctggcg ttgttgttgt gtctgttttc cggcgggtta gcgatgtatg gctacctgcg
    32221 ctggttacgc aatgaaaaag cgatgcgtct gaaagaggac ctgccctaca ccaacagctt
    32281 attaatcatt agcttaatat tgatggtcgt ggcggtgatc gttatgggac tggtgttgta
    32341 tgccggatag ccgcaaagcc agacgcattg ccgacccagg actgcagccg gagcgcacat
    32401 cactggcgtg gtttcgtacc atgctgggct acggcgcgtt gatggcgttg gctatcaaac
    32461 acaactggca ccaggcgggc atgttattct ggatttcgat tggcattctc gccatcgtgg
    32521 cgctgatcct ctggcactac acccgtaatc gcaatttaat ggatgtcacg aatagcgatt
    32581 tttcccaatt tcacgtagtc cgtgacaaat ttttgatctc cctcgcggtg ttatctctcg
    32641 caatactgtt tgctgtaacg catatacatc aacttatcgt atttattgag agagtcgcat
    32701 gacaggaagt caggtcattg atgctgaaga ggacaggcat aaactggttg tagagtacaa
    32761 ggatgctctg caacctgccg atttttatca caatttcaaa cagcggggca tccgcgccgt
    32821 acagctcatt ccctatcttg aatttgatga ccggggcgat ctgacggccg cttcggtgac
    32881 agcagagctg tgggggaaat ttttaattgc cctgttcgag tgttgggtgc gggcagatat
    32941 cagtcgtatc tcgattgagc tttttgacgc caccttacaa aaatggtgtg gcagcgaaaa
    33001 cccgcaaccg cgccgtgact gccaggcgtg cgactggcat cgcctgtgcc cgcacgctcg
    33061 ccaggagacg ccagacagcg tgctttgtgc gggctatcag gcgttttatt cttactccgc
    33121 accgcatatg cgggtgatgc gtgatctgat aaagcagcat cgttctccca tggagctgat
    33181 gacgatgctg cggtgacgtg cgcttaaacg ggctgcccct gatccgacat ccgcgtcgcc
    33241 agcggcagcc agcacagcac gatcaacaat cccattaagg tcatcaacaa ccccaggctg
    33301 ccctgaccgg tttgcggcaa catcgcagag agcgacgcca gcacgccgga accaatgttt
    33361 tgcagaccgc cgaccagcgc gccagccgtg cccgccagga aggggaacgg ctccatcgcg
    33421 ccgctggtcg ccagcggaaa cagcatcccg gcaccgaaaa agaacagcgc ggcgggaacg
    33481 agcagcgtcc agacattcat cacgccaaac cagtcgggga tccacatcag caagccagcc
    33541 agcaggcagc agataaccga ctgccacatt aacgtggaga agcgtttatt gggacgtccg
    33601 gcaaaccatg cgccaaaaaa cgctgccgga atcggcagaa taaacaaaat actgaccgtc
    33661 atactgctca gccctaacac cgcgcccatc agcacgccgg agcaggcttc aaaggcggca
    33721 atcccggcca gaccgccaat cagcatcagc aaataacagt taaaaccgct gttaccgaaa
    33781 agcgttttat aactggtaag caggcgcgtg cgcggtgcat cgaccggacg cgtttccggc
    33841 atccagcggg ccatactgaa ggtcacacca gcacaaagaa ccaacaaaaa gagataacag
    33901 gcgcgccagt tccacatcgt atccagcaga ccgccgatta gcggtgcgag caacggactg
    33961 acgagaatcc ccatgtttaa caggctgtta gcatggcgca actgtgtccg ttcatataaa
    34021 tctcgcggca aagtacgcgc cattacgccg ccaacgccgg tgcccatccc ctgcatcgcg
    34081 ctggcggcaa tcaacaccgt caaactggag gtcgtgaccg cgaccagcgt tgccagcata
    34141 aaaatggaca ttccgacgag gatcaccggt cggcggccca cgcggtcgga aatcgggcca
    34201 taaaacagct gtgagacacc gtaagtcagc agataagcgc ccattacgct ctgcaccgcc
    34261 ccttcacgga cgttgagatc gcgcgccata tcggcaatag ctggaatata aatggtttgc
    34321 gccatctgac cgacggccac gagtaatacc aacatcaata acaaattgac gtttctttgc
    34381 cttttcatta tcacggatgc ttttataaaa ataattagaa taagtcacta cgcattccca
    34441 taaatgcggg agaggaatct atcacattga tgaatgttag ccagatatac gccaggaatg
    34501 gggaattgtt tagcggcaga atatgtaaac aaaagcggca ataaatgttg ccgggaggga
    34561 ttacgtgcga actgtaaccc tctccccaaa ggggcgaggg ggaaaccacc gttccctttc
    34621 agaaaatgag ctaaacattg cacgggacgg tcccctcgcc cctttgggga gagggctagg
    34681 gtgaggggca caggcagcgc aggtgcaaac attctgctgg tagaagaagc ccccaactcc
    34741 aacccgcacg ctaaatataa aaggggagcg gttacccgct cccctttggt gcgacttgaa
    34801 tctgaattac ttcaggtatt tcagaacagc atcaagcagt tgcagtgctg caacaatgag
    34861 tttgaggata agaatggcga tatccaccag gctcatacgc gtctcctgtg gttcaggagc
    34921 actgccagct gacgtacctt tccgctgcct gttgccagct gttgtgcacg cggtatctta
    34981 aggagaaacg ttgtgcttgt tgacataaca cagtgtgctc acgcggggtt aaggcgctga
    35041 cggcaccacc cgtttcagcc aggacttagt gcgccgggta acgatgcggc ccccgcataa
    35101 cacattgcgt acagtgatat tatagaagtt tactgtataa ataaacagta atatttggac
    35161 aaaacgcaaa ctgtgatcga tactcaacta tgccctaaat acgtcaaaat tcgtgccgaa
    35221 attgcgcgtt ctgcgcggaa cacgtatact ttcagtgttg acataataca gtgtgctttg
    35281 cggttaccag ccgcaggcgg ctgatgaaac ctcgctccgg cggggttttt tgttacctgc
    35341 aattcagtac aaaacgtgat cagcccctca attttccctt gctgaaaaat tttccattgt
    35401 ctcccctgta aagctgtgct tgtataaata ttgttaaaca caaaaccaac aaggtcccca
    35461 atgactactt ccatgttcaa cgcaaaacta ctaccaactg cgctatccgc cgcagtggtc
    35521 gtcgtgcgtg tggtggtggt cgtcggcaat gcgccgtagg gactggaaca acacacgatt
    35581 ccaaaacccc gccggcgcaa accgggcggg gtttttcgtt taagcacctc ccggaaagtc
    35641 ggcccagaag aaaaggactg gagcatggca agttcgggca caacatcgac gcgtaagcgc
    35701 tttaccggcg cagaatttat cgttcatttc ctggaacagc agggcattaa gattgtgacg
    35761 ggcattccgg gcggttctat cctgcctgtt tacgatgcct taagccaaag cacgcaaatc
    35821 cgccatattc tggcccgtca tgaacagggc gcgggattta tcgctcaggg gatggcgcgc
    35881 accgacggta aaccggcggt ctgtatggcc tgtagcggac cgggtgcgac taacctggtg
    35941 accgccattg ccgatgcgcg gctggactcc atcccgctga tttgcatcac cggtcaggtt
    36001 cccgcctcga tgatcggcac cgacgccttc caggaagtgg acacctacgg catctctatc
    36061 cccatcacca aacacaacta tctggtcaga catatcgaag aactcccgca ggtcatgagc
    36121 gatgccttcc gcattgcgca atcaggccgc ccaggcccgg tgtggataga cattcctaag
    36181 gatgtgcaaa cggcagtttt tgagattgaa acacagcccg ctatggcaga aaaagccgcc
    36241 gcccccgcct ttagcgaaga aagcattcgt gacgcagcgg cgatgattaa cgctgccaaa
    36301 cgcccggtgc tttatctggg cggcggtgtg atcaatgcgc ccgcacgggt gcgtgaactg
    36361 gcggagaaag cgcaactgcc taccaccatg actttaatgg cgctgggcat gttgccaaaa
    36421 gcgcatccgt tgtcgctggg tatgctgggg atgcacggcg tgcgcagcac caactatatt
    36481 ttgcaggagg cggatttgtt gatagtgctc ggtgcgcgtt ttgatgaccg ggcgattggc
    36541 aaaaccgagc agttctgtcc gaatgccaaa attattcatg tcgatatcga tcgcgcagag
    36601 ctgggtaaaa tcaagcagcc gcatgtggcg attcaggcgg atgttgatga cgtgctggcg
    36661 caattgatcc cgcaggtgga agcgcaaccg cgtgcagagt ggcaccagtt ggtagcggat
    36721 ttgcagcgcg aattcccgtg tccaatcccg aaagcgtgcg atccattaag ccattacggc
    36781 ctgatcaacg ccgttgccgc ctgtgtcgat gacaatgcga ttatcaccac cgatgtgggt
    36841 cagcatcaga tgtggaccgc gcaagcttat ccgctcaatc gcccacgcca gtggctgacc
    36901 tccggtgggc tgggcacgat gggttttggc ctgcctgcgg cgattggcgc ggcgctggcg
    36961 aacccggatc gcaaagtgtt gtgtttctcc ggcgacggca gcctgatgat gaatattcag
    37021 gagatggcga ccgccagtga aaatcagctg gatgtcaaaa tcattctgat gaacaacgaa
    37081 gcgctggggc tggtgcatca gcaacagagt ctgttctacg agcaaggcgt ttttgccgcc
    37141 acctatccgg gaaaaatcaa ctttatgcag attgccgccg gattcggcct cgaaacctgt
    37201 gatttgaata acgaagccga tccgcaggct gcattgcagg aaatcatcaa tcgccctggc
    37261 ccggcgctga tccatgtgcg cattgatgcc gaagaaaaag tttacccgat ggtgccgcca
    37321 ggtgcggcga atactgaaat ggtgggggaa taagccatgc aaaacacaac tcatgacaac
    37381 gtgattctgg agctcaccgt tcgcaaccat ccgggcgtaa tgacccacgt ttgtggcctt
    37441 tttgcccgcc gcgcttttaa cgttgaaggc attctttgtc tgccgattca ggacagcgac
    37501 aaaagccata tctggctact ggtcaatgac gaccagcgtc tggagcagat gataagccaa
    37561 atcgataagc tggaagatgt ggtgaaagtg cagcgtaatc agtccgatcc gactatgttt
    37621 aacaagattg cggtgttttt tcagtaaccg ctcaaggctt gaacaacatc gcgcttatcg
    37681 ttaaggtaag cgcgtttttt tttatccgcc aggacaagac catgatcacc gttgccctta
    37741 tagacgatca cctcatcgtc cgctccggct ttgcgcagct gctggggctg gaacctgatt
    37801 tgcaggtagt tgccgagttt ggttcggggc gcgaggcgct ggcggggctg ccggggcgcg
    37861 gtgtgcaggt gtgtatttgc gatatctcca tgcccgatat ctccggtctg gaactgctaa
    37921 gccagctgcc gaaaggtatg gcgacaataa tgctctctgt tcatgacagt ccggcgctgg
    37981 ttgagcaggc gcttaacgcg ggggcgcgcg gctttctctc caaacgctgt agccctgacg
    38041 aactgattgc ggcggtgcat acggttgcca cgggcggctg ttatctgacg ccggatattg
    38101 ccattaaact ggcatccggt cgtcaggacc cgctgaccaa acgtgaacgc caggtggcgg
    38161 aaaaactggc gcaaggaatg gcggtgaaag agattgccgc cgaactgggc ttgtcaccga
    38221 aaacggtaca cgtccatcgc gccaatctga tggaaaaact gggcgtcagt aacgacgtag
    38281 aactggcgcg ccgcatgttt gatggctggt gatgaagacg ttgttctccc gcttaattac
    38341 cgttattgcc tgctttttta tcttctctgc cgcatggttt tgcctgtgga gtatcagcct
    38401 gcacctggtt gagcgccctg atatggcggt gctgttattt ccgtttggtc tgcggctagg
    38461 gctaatgctg caatgcccgc gcggatactg gcccgtattg ctgggtgcgg aatggctgct
    38521 gatttactgg ctaacgcagg cggtcggttt aacccatttt ccgttattga tgatcggtag
    38581 tttactgacg ttactgcccg tagcgctgat ctcgcgctat cgccatcagc gtgactggcg
    38641 caccttgctg ttacaggggg cggcgttaac ggcggcggcg ttgttgcagt cgctgccctg
    38701 gctttggcac ggcaaagagt cgtggaatgc gctgttgctg actttaactg gcggcctgac
    38761 gctggccccg atatgtctgg tgttctggca ctatctcgcc aataacacct ggctgccgct
    38821 cggcccgtca ctggtttctc aaccgatcaa ctggcgcggg cggcatctgg tctggtactt
    38881 gctgttgttt gttattagtc tctggctcca gttgggattg ccggacgaac tgtcgcgctt
    38941 tacgccattc tgcctggcgc tgccgattat cgcgctggcc tggcactacg gttggcaagg
    39001 ggcgctgatc gcgacgttga tgaacgccat cgcgctgatc gccagtcaaa cctggcgcga
    39061 tcatccggtg gatttattgc tctcgttgct ggtgcaaagt ctgacagggt tgttactggg
    39121 cgctggcatc cagcggttgc gtgaacttaa ccagtcgctg caaaaggaac tggcgcgcaa
    39181 tcagcatctg gctgaacggt tgctggaaac cgaagagagc gtgcgtcgtg atgtggcgcg
    39241 tgagctgcat gatgatatcg gtcagaccat cactgctatt cgtactcagg cgggcattgt
    39301 tcagcggctg gcggcagata acgccagcgt gaagcagagc gggcagctca tcgaacaact
    39361 atcgctgggc gtttacgacg cggtgcgccg tttgttgggt cggttacgtc cgcgccagtt
    39421 ggatgatctc accctggagc aggccatccg ctcactgatg cgggaaatgg agctggaagg
    39481 gcgcggtatt gtcagccatc tcgaatggcg aatcgatgaa tcagcattaa gtgaaaacca
    39541 gcgcgtgacg ctgtttcgtg tctgccagga agggctgaac aacattgtga aacatgctga
    39601 tgccagcgcg gtcacgctgc aaggctggca gcaggatgaa cggttgatgc tggttattga
    39661 agacgatggc agcggtttgc cgccgggttc cgggcaacaa ggttttggcc tcaccggaat
    39721 gcgcgagcgc gtaacggcgc tgggtggcac attacacatt tcctgtctgc acggcacgcg
    39781 tgtcagcgtt tctctacctc aacgctatgt ctaaggtttg atgatgttgc cgtttctgaa
    39841 agcgcctgcc gatgcgccat taatgactga taaatatgaa attgatgccc gctatcgcta
    39901 ctggcgtcgg catattctgc tgaccatctg gctgggttac gcgctgtttt acttcacgcg
    39961 taaaagtttt aacgccgccg taccagaaat ccttgctaac ggcgtgctca gccgtagcga
    40021 tatcggcctg ttagcgaccc tgttttacat tacctacggc gtgtcgaagt ttgtctccgg
    40081 cattgtcagc gatcgctcaa atgcccgtta ttttatgggg atagggctta tcgccacggg
    40141 catcatcaac attctgtttg gcttctcgac gtcgctatgg gcgtttgccg tgctctgggt
    40201 gctgaacgcc tttttccagg gctggggttc accggtgtgt gcgcgtctgt taacggcctg
    40261 gtattcacgt accgagcgcg gcggttggtg ggcattatgg aacacggcgc ataacgtcgg
    40321 cggcgcactc attcccattg tgatggcagc gagcgcgctg cattacggct ggcgtgccgg
    40381 gatgatgatt gctggttgta tggcgatagt cgtggggatt tttctctgct ggcggctacg
    40441 cgatcgcccg caggcgttag gtttaccggc ggtcggtgaa tggcggcacg acgcgctgga
    40501 aattgctcaa caacaagaag gggcagggtt gacgcgtaaa gagatcctga ccaaatatgt
    40561 gttgctgaat ccgtatatct ggctgctttc gttttgctat gtgctggtct atgtggtccg
    40621 ggcggcgatc aacgactggg gcaatttgta tatgtccgag acactgggcg tcgatctggt
    40681 cacggcgaat acggcagtga cgatgtttga actgggcgga tttatcggtg cgctggtagc
    40741 cggttggggc tcggacaaat tgtttaacgg caaccgtggg ccgatgaatt tgattttcgc
    40801 cgccggaatt ttgctttcag tcggctcgct gtggctgatg ccatttgcca gctacgtgat
    40861 gcaggcaacc tgcttcttca ccattggttt ttttgtcttt ggcccgcaga tgttaatcgg
    40921 catggcggcg gcagagtgtt cccacaaaga ggcggcaggg gcggcgacgg ggtttgtcgg
    40981 cttgtttgct tatctggggg cgtcgcttgc tggttggccg ctggcgaaag tactcgatac
    41041 ctggcactgg agcggatttt ttgtggttat ctctatcgcc gccgggattt ccgcactgct
    41101 gttactgccc tttttgaacg cccaggcacc gcgcgaagcg tgatgcatct caccttttca
    41161 cttcatatcc ggcaaaacta agaaattttc caggttttgc ctggacgcta tctcaggcct
    41221 gatttgctgc tgatttttac aatgcatgcc tcacgcaggt attcatttca ggagtaaccc
    41281 atgctggctt tcttaaacca ggttcgcaag ccgaccctgg accttccgct cgaagtgcgg
    41341 cgcaaaatgt ggttcaaacc gttcatgcaa tcctacctgg tggtctttat cggctatctg
    41401 acgatgtacc tgattcgcaa gaactttaac atcgcgcaga acgacatgat ctcgacctac
    41461 gggttgagca tgacgcagct ggggatgatc ggcctgggtt tctccatcac ttatggcgtg
    41521 ggtaaaacgc tggtttccta ctacgccgac ggcaaaaaca ccaaacaatt cctgccgttc
    41581 atgctgatcc tgtctgccat ttgtatgctg ggcttcagtg ccagtatggg cagcggctcg
    41641 gttagcctgt tcctgatgat tgccttctac gccttaagcg gctttttcca gagtaccggc
    41701 ggttcgtgca gttactccac catcaccaaa tggacaccgc gtcgtaagcg cgggaccttc
    41761 ctcggtttct ggaacatttc ccataacctt ggcggcgcgg gtgcggctgg tgtggcgctg
    41821 ttcggcgcaa attacctgtt cgacggccac gtgatcggca tgtttatctt cccgtcgatt
    41881 atcgcgttga ttgtcggttt tatcggcctg cgttacggca gcgactcccc ggaatcttat
    41941 ggcctcggca aagcggaaga gctgttcggc gaggagatca gcgaagagga caaagagaca
    42001 gaatctaccg atatgaccaa gtggcagatc tttgttgagt atgtgctgaa aaacaaagtg
    42061 atctggctgc tgtgcttcgc caacattttc ctctatgtgg tgcgtattgg tatcgaccag
    42121 tggtcaaccg tgtacgcgtt ccaggaactg aaactctcta aagcggtggc gattcagggc
    42181 tttacgctgt ttgaagctgg tgcgctggtc ggtacgctgc tgtggggctg gctctctgac
    42241 ctggcgaacg gtcgccgtgg cctggtggcc tgcatcgcgc tggcgctgat tatcgccacg
    42301 ctcggtgtgt atcaacatgc cagcaacgaa tatatctatc tggcttctct ctttgcgttg
    42361 ggtttcctgg tctttggccc gcaattgttg attggtgtgg ctgctgttgg ctttgtacct
    42421 aaaaaagcga ttggcgctgc cgatggtatt aaaggcacct ttgcttacct gattggtgac
    42481 agctttgcca agttaggtct gggaatgatt gccgatggga cgccggtatt cggccttacc
    42541 ggctgggcag gcaccttcgc cgcacttgat atcgccgcga ttggttgtat ctgcctgatg
    42601 gcgatagtgg cggtgatgga agaacgcaaa atccgccgcg agaaaaaaat tcagcagttg
    42661 acagtggcat aaacgtaact ggtgactttt gcccggcatg acgccgggct tttttattat
    42721 tccgtgactt ccagcgtagt gaaggcaaac ttctcgccat caaatagccc ctgactggtt
    42781 agttttagcg cggggatcac tggcagagaa agaaacgcca tctgaataaa cggctcatcg
    42841 ggtaacggac cgcattcacg ggcggcggct ttcaaggcgt caatttgttc cgccagcaac
    42901 tgcgccgtgt cggtgctcat caggccggca atgggcaacg gcagatgact ttggacctgg
    42961 ccgttacgta ccacgcacag cccgccgcca tcctgaatca cctgattgac cgccagcgcc
    43021 atctcttcgg cactgcgacc gatcaccaca atattatggc tgtcatggct gaccgtcgca
    43081 gccagcgcac cttcattcag tccaaagccg ccaagcaaac cacaagccgg agccagccgt
    43141 tgcccgtaac gctcaagtac ggcaataaag ctcacatcat cgcgatcaaa accattttcg
    43201 ctgtagacgc tggagtggga gtgcgtaatc aattcgttat ggatgacgtc aatgacccga
    43261 tagcgttttc cgggcgtaaa ttgcagggca aagtcgctgg cggaaactgg ctggcgggca
    43321 atggtgttgc catatggcgg agcggattgt gccagtctcg ccgactcttc cgcctgtaag
    43381 gtttgcgcat caatcggctc gcctttcacc agtacctgct gcaccgtgac cttacgcgca
    43441 tcgctcaaca ggacgatatc ggcctgcttg ccgggtgcca gtaagccgag gtgattcaga
    43501 ccaaagtggc gcgccgtcga ccagctggcg acgcgatatg ccacatgcag cggcacattg
    43561 tgttgttcga tcaggcggcg aattaaggca tcgatgtgtc cttcatgggc gatctcccac
    43621 gggttacggt catcggtaca gagcatgcat tgcgggctgt taaattcgtt gatcaacggt
    43681 gccagcgcgt tgagattgcg ggcagcggac ccttcgcgga tcatcaacga catgccgagt
    43741 tgtaatttcc ggcgtccttc ttccagctga taactttcgt ggcagttttc aatacccgca
    43801 gtaatatagg cgttaagttc tttaccaccc aaacccgggc agtgaccgtc cagcgtcagg
    43861 tggcgaaatg catccagttt atcgagcagc gcattctgcc cgctaattac gccagggtag
    43921 tccatcattt ctgcaaggcc ggtaacctgc ggatggtccc gccaggcgag catctgttca
    43981 agggtaaaac tggcaccgtt aacatcgcag ccttccaggg cgggtacgca agagctgacc
    44041 tgtaagtact ggttttgcct tgcctgttcg gcacagcggg caaaccaggc gaatccggct
    44101 tctcccatca cgttgacgat ttcatgaggg tcgcaaataa cggtcgtcag gccgcgcggc
    44161 agggtagcgg tttcaaaagt gaccggcgtc atcatgctgg attcaatatg caggtgagca
    44221 tcaataaacc ctggcaccgc cgttgcgccg cgagcatcaa tccgctgcaa agccggagca
    44281 tcagcgtatt ctgcgccaac accggcaatg taacgtcctt taatcacaat tgggccggaa
    44341 atttctccgc cattgatcag gtcgagaata gagacattat caataatata atcggcaaca
    44401 gcgtcgccac gggaaacggc taacaattcc tggtattcag cccggctaat gtgatgaaat
    44461 ttatggttaa tagaattatt catatttttc tcattttgat tttatcaatt tgattattat
    44521 ttatcataaa gaaacgtcgc gagattatta aagaatatcc attaatgtgc aattgaaatg
    44581 tgataattat cactgaaccg ttgttgtcat ttgttttaca aaagcaaggg attaaatctc
    44641 ctccaatgga caaaaaaatg aataatgaca ataccgatta cgtgagtaat gaatcaggga
    44701 cgctttcgcg attatttaaa ctacctcagc atgggaccac cgtccgcaca gaattgattg
    44761 cggggatgac cactttttta accatggtgt acatcgtttt tgtgaacccg caaatcctcg
    44821 gcgcggcaca aatggacccg aaagtggtgt ttgttaccac ctgtttgatt gccggtatcg
    44881 gcagtattgc gatggggata tttgctaact tacccgtggc gctggctccg gcaatggggc
    44941 tgaacgcctt ctttgccttc gtggtcgtgg gggcgatggg catctcctgg cagaccggga
    45001 tgggcgcaat attctggggc gcagttggac tatttttgct cacgctgttt cgtatccggt
    45061 actggatgat ctccaacatt cccttaagtt tacgtattgg tatcaccagc ggaattggat
    45121 tatttatcgc cttaatggga ttaaaaaata ctggcgttat tgtcgccaat aaagacacgc
    45181 tggtgatgat tggcgattta agttctcacg gcgtgttgtt aggtatttta gggtttttta
    45241 ttataaccgt gttgtcatca cgtcattttc atgccgcggt gctggtttct attgtggtga
    45301 cgtcttgctg tggattattt ttcggtgatg ttcattttag cggcgtctat tccattccgc
    45361 ctgatattag cggcgtcatt ggtgaagtag atttgagcgg cgcgttaaca cttgaactcg
    45421 ccggtatcat tttctccttt atgctgatca acctatttga ttcatcagga acattaattg
    45481 gtgtaactga taaagcgggc ttaatagata gtaacggtaa attccccaat atgaataagg
    45541 cgctgtatgt tgatagcgtc agttcggtgg cgggggcgtt tatcggcacc tcgtctgtta
    45601 ctgcctatat tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg ctgactgcgg
    45661 ttgtggttgg cgttatgttc ctgttagtta tgttcttctc accgctggtg gcgatggttc
    45721 ctccttacgc aaccgccgga gcgttaatct ttgttggcgt gctgatgact tcgagcctgg
    45781 cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc gtttattacc acggtgatga
    45841 tgccctttac tttctcgatc accgaaggga ttgcactcgg ctttatgtcg tactgcatca
    45901 tgaaagtatg caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg gtcgcagctc
    45961 tgtttgcact gaagattatt ctggtggatt agtaactggc gagggtttcc agatatcatg
    46021 agttctgatt acgcaggaga actcatgatc tggataatgc tcgccacgct ggcggtagtg
    46081 tttgtggttg gttttcgggt gctgacatcc ggggccagaa aagcgattcg ccgtctcagc
    46141 gatcggctga acatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat gggaaagtca
    46201 gctggtgacg aatttttacg ttatttgcat cgtccggatg agtcgcacct gcaaaacgcc
    46261 gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca gaacctgctg
    46321 caatggcatc ggattttaca aaaagctcgc ctcgccgcgc cgattaccga cgctcaggtc
    46381 aggctggcgc taggttttct gcgcgaaacc gaacctgaaa tgcaggatat taatgctttt
    46441 cagatgcgct ataacgcgtt ctttcagcct gccgagggcg ttcactggtt gcattgaaac
    46501 cgtttcgtct tatcagccta cagaccgcgc ctgacctgtc gggtggataa gacgttcacg
    46561 ccgcatccgg taatctttgc cctgttccgt ttcgatctta actccagctc aagcaacacc
    46621 gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa tgttaaagtg gtcggctttt
    46681 cccctgaaac atgccacggg taacaccatg agtgaattta ttgccgaaaa ccgcggcgcg
    46741 gatgccatca cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg tgtcgcctgt
    46801 ctgattatcg ttgagttttt gcccgtcagt ttgttgacgc caatggccca ggatttaggc
    46861 atttcggaag gggttgccgg gcaatcggtg accgtgaccg cctttgtggc aatgtttgcc
    46921 agtttgttta ttacccagac aattcaggct actgaccgcc gctacgttgt tattttgttt
    46981 gccgttttgc tgacgctctc ctgcttgctg gtttcctttg ctaactcatt cagtttgctt
    47041 ttaatcggtc gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat gtcggcgtcg
    47101 ctgaccatgc gtctggtgcc gccgcgtacg gtgccgaagg cgctgtcggt gatcttcggc
    47161 gcggtttcta ttgcgctggt gattgccgcg ccgttgggca gttttttagg cgagcttatc
    47221 ggttggcgca atgtctttaa tgcggcggcg gtgatgggcg tgctgtgtat tttctggatt
    47281 atcaaatcat tgccttcact gccaggcgaa ccctcgcatc agaaacaaaa tactttccgc
    47341 ttattacaac gtccgggtgt gatggcaggg atgatcgcca tcttcatgtc tttcgccggg
    47401 cagtttgctt tcttcacgta tattcgcccg gtgtatatga acctggcggg attcggcgtg
    47461 gatggcttaa cgctggtgct gttgagtttt ggtatcgcca gctttattgg tacgtcgctt
    47521 tcgtcgttca ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc gttaatactg
    47581 gctgtgagtg cgttggtact gacgttgtgg ggaagcgata aaatcgttgc taccggcgtg
    47641 gcgattatct gggggctaac ttttgcattg gttcccgtcg gctggtcaac gtggatcacc
    47701 cgctcgctgg ccgatcaggc agaaaaagcc gggtctattc aggtggcggt tattcagctt
    47761 gctaatacct gtggcgcggc aatcggcggt tatgcgctgg ataatattgg tctgacttcg
    47821 ccgttgatgt tgtccggcac attgatgttg ctgactgcat tgttggttac tgcaaaggtg
    47881 aaaatgaaga aatcctgata tcaggtaaag aagcgttggt cagtagttgt tgctgttctt
    47941 acatatccgg gcattctacc ttacccaacg cttcccaata attgctctga ttattacgtt
    48001 ccatcgccac gatggcctcg cgtatatgtt gtttattgtt ttcatctgac aacattaact
    48061 tttcccacgc ttcatctgat aatgacgcct gtggcgtgca aattttacgt aaatcttgta
    48121 ataccgtggt tttctctttt gcggactgca gtgagctaaa aggcgactcg gcgtatacga
    48181 tgcctggcat agcgaaaaaa gtgaaaataa gcagtagtgt cgttggtttc atggattcaa
    48241 ccttttacaa atgtgtatcg aatgaagcat aagtgtagtt tgctttcatc gctctcatca
    48301 tgctgtacga aacgcgcttg cgtggtcagt aagaagtgcc agactttata ttccactttt
    48361 attccttttt attcttatcg atagcgtttc gttttttaaa ccgcagcgac cttaccgcta
    48421 tagtcaggta atcattaata aaaggataaa aaaatgaaac tgacaacaca tcatctacgg
    48481 acaggggccg cattattgct ggccggaatt ctgctggcag gttgcgacca gagtagcagc
    48541 gatgcaaaac acattaaagt tggcgttata aatggcgcag aacaagatgt cgcggaagtc
    48601 gctaaaaagg tggcaaaaga gaaatatggc ctcgatgttg aactggtagg atttagcggt
    48661 tcgttgctac cgaacgatgc gacaaatcat ggcgaacttg atgcgaatgt cttccagcat
    48721 cgaccctttc ttgaacaaga taatcaggcg catggttata aactggtagc ggtgggaaat
    48781 acgtttgtct tccctatggc gggttattcc aaaaaaatca aaaccgtggc gcaaataaaa
    48841 gaaggcgcga cagtggcgat ccccaacgac ccgaccaacc ttggtcgcgc acttttactg
    48901 ttgcaaaaag agaagctaat tactctgaaa gagggaaaag ggttattacc taccgcgctg
    48961 gatataaccg ataatcctcg tcatttgcag attatggaac tcgagggggc gcagctcccg
    49021 cgcgtactgg atgatcccaa agttgatgta gcgattatca gcaccactta cattcagcag
    49081 acagggcttt ctccggtgca cgacagcgta tttattgaag ataagaattc gccgtatgtg
    49141 aatattttgg tggcacggga agataataag aatgcagaaa acgtgaagga atttctgcaa
    49201 tcttatcaat cacccgaagt cgctaaagcg gcagaaacca tttttaacgg tggcgcggtg
    49261 cctggctggt aattctcact catgccgcgc cggacgattt atttttctgg cgcggcgacg
    49321 ggaatccatt gaaatcaaaa tcaccgatga cagaatcagc agcgttccca gccagtccgg
    49381 taatgtgaac gtgatcccca gcaacagcaa agagagtagc gcgctactta atggttctgc
    49441 acagctcaaa atgctggctt ttggaccgcc aattaattgt gctcctttca ggtacagact
    49501 aaatgtcagg gacgtaccaa tgaccaccaa ataaaaaaac gccagaatca aactgccgtt
    49561 aacgacaaag tttgttcctt gtctggcata aaaaggcaac agaatcagac cgccaatcag
    49621 catactccag ccgacgactg gtaacgtgcc atagcgggca attagcgttg agggataggt
    49681 ggtatagaat gcagcagcaa atgccgaggc aatgccccag aacaacgcgg caggagagat
    49741 cgataatgac gtcggattac cgtgtgtcac caataaaaaa gtcccgacca gcgatgtcaa
    49801 aatagcgcag aaaaccagaa tgcccgggcg cgatttacgc accagtgaga accaggcgac
    49861 gataatcgtc ggtgagagga attgcagcac cgttgccgtg gctgcgttcg atttttcgat
    49921 ggttagcaaa aaagtgagct gtacagttag cgcgccaacc acggaaaaaa tcagcaggct
    49981 aatggcatct ttatgattgt taataataga aaagatttta tcgccatgaa caaatgacag
    50041 cgtcagtaga atcaaaccgg cgaatatcaa acgcgtcata gtcaaaaact gcgacgacat
    50101 ctggctttgc tccatgatgt attgcgcgca gacccctgaa cttccccaca acacggcggc
    50161 aatcagaacg ttcagcatcc cctttctggt ggaacccatt tttccctctg catactgttt
    50221 tgtcgtttat gtatgcaggc atgatagcaa aaatggcgag gatggctctg tttgcgccac
    50281 gccgattttt cttagcccag actggtgagc taaatatctt taataaacag caggcaaatc
    50341 atcgcaatac ccaacatccc tatcgccagc cagaacaacg catggtagct ccagatttca
    50401 accataattc cgtcaacaga gccggcgata atccagccga cgcgtgaagt atttgcatat
    50461 aacgttgtcg cagagcctat tttttcaggc atcaagtcct gaaaataaag catgccgata
    50521 ccacagagaa taccaaggaa gatggcatta agaatttgca attccagctc aaccgccgga
    50581 gtcgtcgcca tgagtacgct ggcgtaaaaa cacattccac tcacgatagc aatgagcatt
    50641 aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat cggaatttcc
    50701 agaccggcag cgataccaat catttctcca gccagtttat cggttagatg cagttcatca
    50761 ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat cattgaacag
    50821 accacaaaaa gtagccgcgt atcccgcttc ctgtgggtgg agggtaaaat ttctacgggt
    50881 tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac tatcagtccg
    50941 caaacaacaa atgcgatggc tgcggtgaga tacatcactt taaaactaaa tcccattgcc
    51001 agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc acgtaaaaat
    51061 gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc gagggcgaac
    51121 atttgcgggt ttgcggtgga agcaaaacta ctcagaagta cgcccgttga gaggagaatg
    51181 aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa gcaacatagc
    51241 agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa ttgactgacc
    51301 agaattccca taatagcgct accggtgaag aaaaaaccta ccattatagg acgggctttc
    51361 agttcatctg cgaggaatat acttagggta ggagtctgaa gagcgcccgc aatacccgtc
    51421 agaaaggcga caagtaaaaa tgccgcggca gtgagatcaa gtatttttga tggagtggta
    51481 gccgtttttt gcattcttct gaatcaccgg gcgaaaaaat ataactgagt tacaaataag
    51541 taccagtcga atgagtattg tattaagaat ttttgagtat cgctgataca accagaaagc
    51601 aatgctagcg caatattata gggccagcaa ttgag
//
LOCUS       DAZFVO020000013        49461 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFVO020000013 DAZFVO020000000
VERSION     DAZFVO020000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 49461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.013"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(142..846)
                     /gene="ybhL"
                     /locus_tag="ACHKNB_RS03400"
                     /old_locus_tag="pgaptmp_000680"
     CDS             complement(142..846)
                     /gene="ybhL"
                     /locus_tag="ACHKNB_RS03400"
                     /old_locus_tag="pgaptmp_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4194255.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(983..1435)
                     /gene="moaE"
                     /locus_tag="ACHKNB_RS03405"
                     /old_locus_tag="pgaptmp_000681"
     CDS             complement(983..1435)
                     /gene="moaE"
                     /locus_tag="ACHKNB_RS03405"
                     /old_locus_tag="pgaptmp_000681"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HGU4194256.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1437..1682)
                     /gene="moaD"
                     /locus_tag="ACHKNB_RS03410"
                     /old_locus_tag="pgaptmp_000682"
     CDS             complement(1437..1682)
                     /gene="moaD"
                     /locus_tag="ACHKNB_RS03410"
                     /old_locus_tag="pgaptmp_000682"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HGU4194257.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1675..2160)
                     /gene="moaC"
                     /locus_tag="ACHKNB_RS03415"
                     /old_locus_tag="pgaptmp_000683"
     CDS             complement(1675..2160)
                     /gene="moaC"
                     /locus_tag="ACHKNB_RS03415"
                     /old_locus_tag="pgaptmp_000683"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HGU4194258.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2163..2675)
                     /gene="moaB"
                     /locus_tag="ACHKNB_RS03420"
                     /old_locus_tag="pgaptmp_000684"
     CDS             complement(2163..2675)
                     /gene="moaB"
                     /locus_tag="ACHKNB_RS03420"
                     /old_locus_tag="pgaptmp_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HGU4194259.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2697..3686)
                     /gene="moaA"
                     /locus_tag="ACHKNB_RS03425"
                     /old_locus_tag="pgaptmp_000685"
     CDS             complement(2697..3686)
                     /gene="moaA"
                     /locus_tag="ACHKNB_RS03425"
                     /old_locus_tag="pgaptmp_000685"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HGU4194260.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3674..3818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4083..4991
                     /gene="yvcK"
                     /locus_tag="ACHKNB_RS03430"
                     /old_locus_tag="pgaptmp_000686"
     CDS             4083..4991
                     /gene="yvcK"
                     /locus_tag="ACHKNB_RS03430"
                     /old_locus_tag="pgaptmp_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HGU4194261.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5183..7204)
                     /gene="uvrB"
                     /locus_tag="ACHKNB_RS03435"
                     /old_locus_tag="pgaptmp_000687"
     CDS             complement(5183..7204)
                     /gene="uvrB"
                     /locus_tag="ACHKNB_RS03435"
                     /old_locus_tag="pgaptmp_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGU4194262.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7783..8460)
                     /gene="bioD"
                     /locus_tag="ACHKNB_RS03440"
                     /old_locus_tag="pgaptmp_000688"
     CDS             complement(7783..8460)
                     /gene="bioD"
                     /locus_tag="ACHKNB_RS03440"
                     /old_locus_tag="pgaptmp_000688"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4194263.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8453..9208)
                     /gene="bioC"
                     /locus_tag="ACHKNB_RS03445"
                     /old_locus_tag="pgaptmp_000689"
     CDS             complement(8453..9208)
                     /gene="bioC"
                     /locus_tag="ACHKNB_RS03445"
                     /old_locus_tag="pgaptmp_000689"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HGU4194264.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLEQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9195..10349)
                     /gene="bioF"
                     /locus_tag="ACHKNB_RS03450"
                     /old_locus_tag="pgaptmp_000690"
     CDS             complement(9195..10349)
                     /gene="bioF"
                     /locus_tag="ACHKNB_RS03450"
                     /old_locus_tag="pgaptmp_000690"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HGU4194265.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10346..11386)
                     /gene="bioB"
                     /locus_tag="ACHKNB_RS03455"
                     /old_locus_tag="pgaptmp_000691"
     CDS             complement(10346..11386)
                     /gene="bioB"
                     /locus_tag="ACHKNB_RS03455"
                     /old_locus_tag="pgaptmp_000691"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HGU4194266.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFHKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11473..12762
                     /gene="bioA"
                     /locus_tag="ACHKNB_RS03460"
                     /old_locus_tag="pgaptmp_000692"
     CDS             11473..12762
                     /gene="bioA"
                     /locus_tag="ACHKNB_RS03460"
                     /old_locus_tag="pgaptmp_000692"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HGU4194267.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12821..13297
                     /gene="ybhB"
                     /locus_tag="ACHKNB_RS03465"
                     /old_locus_tag="pgaptmp_000693"
     CDS             12821..13297
                     /gene="ybhB"
                     /locus_tag="ACHKNB_RS03465"
                     /old_locus_tag="pgaptmp_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HGU4194268.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13449..14732
                     /gene="ybhC"
                     /locus_tag="ACHKNB_RS03470"
                     /old_locus_tag="pgaptmp_000694"
     CDS             13449..14732
                     /gene="ybhC"
                     /locus_tag="ACHKNB_RS03470"
                     /old_locus_tag="pgaptmp_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HGU4194269.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14966..17227)
                     /gene="ybhJ"
                     /locus_tag="ACHKNB_RS03475"
                     /old_locus_tag="pgaptmp_000695"
     CDS             complement(14966..17227)
                     /gene="ybhJ"
                     /locus_tag="ACHKNB_RS03475"
                     /old_locus_tag="pgaptmp_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HGU4194270.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17410..18843)
                     /gene="ybhI"
                     /locus_tag="ACHKNB_RS03480"
                     /old_locus_tag="pgaptmp_000696"
     CDS             complement(17410..18843)
                     /gene="ybhI"
                     /locus_tag="ACHKNB_RS03480"
                     /old_locus_tag="pgaptmp_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HGU4194271.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18919..19962)
                     /gene="ybhH"
                     /locus_tag="ACHKNB_RS03485"
                     /old_locus_tag="pgaptmp_000697"
     CDS             complement(18919..19962)
                     /gene="ybhH"
                     /locus_tag="ACHKNB_RS03485"
                     /old_locus_tag="pgaptmp_000697"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HGU4194272.1"
                     /translation="MPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDLEI
                     DGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAI
                     ENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTF
                     LNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADK
                     ALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAI
                     TGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIR
                     TTRKIFSGEVYLP"
     gene            20154..21107
                     /gene="ybhD"
                     /locus_tag="ACHKNB_RS03490"
                     /old_locus_tag="pgaptmp_000698"
     CDS             20154..21107
                     /gene="ybhD"
                     /locus_tag="ACHKNB_RS03490"
                     /old_locus_tag="pgaptmp_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4194273.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(21148..22143)
                     /gene="pgl"
                     /locus_tag="ACHKNB_RS03495"
                     /old_locus_tag="pgaptmp_000699"
     CDS             complement(21148..22143)
                     /gene="pgl"
                     /locus_tag="ACHKNB_RS03495"
                     /old_locus_tag="pgaptmp_000699"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HGU4194274.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            22298..23116
                     /gene="ybhA"
                     /locus_tag="ACHKNB_RS03500"
                     /old_locus_tag="pgaptmp_000700"
     CDS             22298..23116
                     /gene="ybhA"
                     /locus_tag="ACHKNB_RS03500"
                     /old_locus_tag="pgaptmp_000700"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HGU4194275.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(23117..24175)
                     /gene="modC"
                     /locus_tag="ACHKNB_RS03505"
                     /old_locus_tag="pgaptmp_000701"
     CDS             complement(23117..24175)
                     /gene="modC"
                     /locus_tag="ACHKNB_RS03505"
                     /old_locus_tag="pgaptmp_000701"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HGU4194276.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(24178..24867)
                     /gene="modB"
                     /locus_tag="ACHKNB_RS03510"
                     /old_locus_tag="pgaptmp_000702"
     CDS             complement(24178..24867)
                     /gene="modB"
                     /locus_tag="ACHKNB_RS03510"
                     /old_locus_tag="pgaptmp_000702"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HGU4194277.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(24867..25640)
                     /gene="modA"
                     /locus_tag="ACHKNB_RS03515"
                     /old_locus_tag="pgaptmp_000703"
     CDS             complement(24867..25640)
                     /gene="modA"
                     /locus_tag="ACHKNB_RS03515"
                     /old_locus_tag="pgaptmp_000703"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4194278.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(25807..25956)
                     /gene="acrZ"
                     /locus_tag="ACHKNB_RS03520"
                     /old_locus_tag="pgaptmp_000704"
     CDS             complement(25807..25956)
                     /gene="acrZ"
                     /locus_tag="ACHKNB_RS03520"
                     /old_locus_tag="pgaptmp_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HGU4194279.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            26085..26873
                     /gene="modE"
                     /locus_tag="ACHKNB_RS03525"
                     /old_locus_tag="pgaptmp_000705"
     CDS             26085..26873
                     /gene="modE"
                     /locus_tag="ACHKNB_RS03525"
                     /old_locus_tag="pgaptmp_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HGU4194280.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            26941..28413
                     /gene="modF"
                     /locus_tag="ACHKNB_RS03530"
                     /old_locus_tag="pgaptmp_000706"
     CDS             26941..28413
                     /gene="modF"
                     /locus_tag="ACHKNB_RS03530"
                     /old_locus_tag="pgaptmp_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HGU4194281.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKSLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            28674..29690
                     /gene="galE"
                     /locus_tag="ACHKNB_RS03535"
                     /old_locus_tag="pgaptmp_000707"
     CDS             28674..29690
                     /gene="galE"
                     /locus_tag="ACHKNB_RS03535"
                     /old_locus_tag="pgaptmp_000707"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGU4194282.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            29700..30746
                     /gene="galT"
                     /locus_tag="ACHKNB_RS03540"
                     /old_locus_tag="pgaptmp_000708"
     CDS             29700..30746
                     /gene="galT"
                     /locus_tag="ACHKNB_RS03540"
                     /old_locus_tag="pgaptmp_000708"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HGU4194283.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            30750..31898
                     /gene="galK"
                     /locus_tag="ACHKNB_RS03545"
                     /old_locus_tag="pgaptmp_000709"
     CDS             30750..31898
                     /gene="galK"
                     /locus_tag="ACHKNB_RS03545"
                     /old_locus_tag="pgaptmp_000709"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGU4194284.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            31892..32932
                     /gene="galM"
                     /locus_tag="ACHKNB_RS03550"
                     /old_locus_tag="pgaptmp_000710"
     CDS             31892..32932
                     /gene="galM"
                     /locus_tag="ACHKNB_RS03550"
                     /old_locus_tag="pgaptmp_000710"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HGU4194285.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            33137..33889
                     /gene="gpmA"
                     /locus_tag="ACHKNB_RS03555"
                     /old_locus_tag="pgaptmp_000711"
     CDS             33137..33889
                     /gene="gpmA"
                     /locus_tag="ACHKNB_RS03555"
                     /old_locus_tag="pgaptmp_000711"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HGU4194286.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(34047..35099)
                     /gene="aroG"
                     /locus_tag="ACHKNB_RS03560"
                     /old_locus_tag="pgaptmp_000712"
     CDS             complement(34047..35099)
                     /gene="aroG"
                     /locus_tag="ACHKNB_RS03560"
                     /old_locus_tag="pgaptmp_000712"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HGU4194287.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            35415..35795
                     /gene="ybgS"
                     /locus_tag="ACHKNB_RS03565"
                     /old_locus_tag="pgaptmp_000713"
     CDS             35415..35795
                     /gene="ybgS"
                     /locus_tag="ACHKNB_RS03565"
                     /old_locus_tag="pgaptmp_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HGU4194288.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            35909..36850
                     /gene="zitB"
                     /locus_tag="ACHKNB_RS03570"
                     /old_locus_tag="pgaptmp_000714"
     CDS             35909..36850
                     /gene="zitB"
                     /locus_tag="ACHKNB_RS03570"
                     /old_locus_tag="pgaptmp_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HGU4194289.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGENPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(36847..37566)
                     /gene="pnuC"
                     /locus_tag="ACHKNB_RS03575"
                     /old_locus_tag="pgaptmp_000715"
     CDS             complement(36847..37566)
                     /gene="pnuC"
                     /locus_tag="ACHKNB_RS03575"
                     /old_locus_tag="pgaptmp_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGU4194290.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(37604..38647)
                     /gene="nadA"
                     /locus_tag="ACHKNB_RS03580"
                     /old_locus_tag="pgaptmp_000716"
     CDS             complement(37604..38647)
                     /gene="nadA"
                     /locus_tag="ACHKNB_RS03580"
                     /old_locus_tag="pgaptmp_000716"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HGU4194291.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(38896..39021)
                     /locus_tag="ACHKNB_RS03585"
                     /old_locus_tag="pgaptmp_000717"
     ncRNA           complement(38896..39021)
                     /ncRNA_class="other"
                     /locus_tag="ACHKNB_RS03585"
                     /old_locus_tag="pgaptmp_000717"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(39052..39127)
                     /locus_tag="ACHKNB_RS03590"
                     /old_locus_tag="pgaptmp_000718"
     tRNA            complement(39052..39127)
                     /locus_tag="ACHKNB_RS03590"
                     /old_locus_tag="pgaptmp_000718"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39092..39094),aa:Lys,seq:ttt)
     gene            complement(39161..39236)
                     /locus_tag="ACHKNB_RS03595"
                     /old_locus_tag="pgaptmp_000719"
     tRNA            complement(39161..39236)
                     /locus_tag="ACHKNB_RS03595"
                     /old_locus_tag="pgaptmp_000719"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39201..39203),aa:Lys,seq:ttt)
     gene            complement(39383..39458)
                     /locus_tag="ACHKNB_RS03600"
                     /old_locus_tag="pgaptmp_000720"
     tRNA            complement(39383..39458)
                     /locus_tag="ACHKNB_RS03600"
                     /old_locus_tag="pgaptmp_000720"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39423..39425),aa:Lys,seq:ttt)
     gene            complement(39462..39537)
                     /locus_tag="ACHKNB_RS03605"
                     /old_locus_tag="pgaptmp_000721"
     tRNA            complement(39462..39537)
                     /locus_tag="ACHKNB_RS03605"
                     /old_locus_tag="pgaptmp_000721"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39502..39504),aa:Val,seq:tac)
     gene            complement(39687..39762)
                     /locus_tag="ACHKNB_RS03610"
                     /old_locus_tag="pgaptmp_000722"
     tRNA            complement(39687..39762)
                     /locus_tag="ACHKNB_RS03610"
                     /old_locus_tag="pgaptmp_000722"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39727..39729),aa:Lys,seq:ttt)
     gene            complement(39765..39840)
                     /locus_tag="ACHKNB_RS03615"
                     /old_locus_tag="pgaptmp_000723"
     tRNA            complement(39765..39840)
                     /locus_tag="ACHKNB_RS03615"
                     /old_locus_tag="pgaptmp_000723"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39805..39807),aa:Val,seq:tac)
     gene            complement(39976..40051)
                     /locus_tag="ACHKNB_RS03620"
                     /old_locus_tag="pgaptmp_000724"
     tRNA            complement(39976..40051)
                     /locus_tag="ACHKNB_RS03620"
                     /old_locus_tag="pgaptmp_000724"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40016..40018),aa:Lys,seq:ttt)
     gene            complement(40216..41007)
                     /gene="cpoB"
                     /locus_tag="ACHKNB_RS03625"
                     /old_locus_tag="pgaptmp_000725"
     CDS             complement(40216..41007)
                     /gene="cpoB"
                     /locus_tag="ACHKNB_RS03625"
                     /old_locus_tag="pgaptmp_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HGU4194292.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(41017..41538)
                     /gene="pal"
                     /locus_tag="ACHKNB_RS03630"
                     /old_locus_tag="pgaptmp_000726"
     CDS             complement(41017..41538)
                     /gene="pal"
                     /locus_tag="ACHKNB_RS03630"
                     /old_locus_tag="pgaptmp_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HGU4194293.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(41573..42865)
                     /gene="tolB"
                     /locus_tag="ACHKNB_RS03635"
                     /old_locus_tag="pgaptmp_000727"
     CDS             complement(41573..42865)
                     /gene="tolB"
                     /locus_tag="ACHKNB_RS03635"
                     /old_locus_tag="pgaptmp_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HGU4194294.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(42998..44263)
                     /gene="tolA"
                     /locus_tag="ACHKNB_RS03640"
                     /old_locus_tag="pgaptmp_000728"
     CDS             complement(42998..44263)
                     /gene="tolA"
                     /locus_tag="ACHKNB_RS03640"
                     /old_locus_tag="pgaptmp_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HGU4194295.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(44328..44756)
                     /gene="tolR"
                     /locus_tag="ACHKNB_RS03645"
                     /old_locus_tag="pgaptmp_000729"
     CDS             complement(44328..44756)
                     /gene="tolR"
                     /locus_tag="ACHKNB_RS03645"
                     /old_locus_tag="pgaptmp_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HGU4194296.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(44760..45452)
                     /gene="tolQ"
                     /locus_tag="ACHKNB_RS03650"
                     /old_locus_tag="pgaptmp_000730"
     CDS             complement(44760..45452)
                     /gene="tolQ"
                     /locus_tag="ACHKNB_RS03650"
                     /old_locus_tag="pgaptmp_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HGU4194297.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(45449..45853)
                     /gene="ybgC"
                     /locus_tag="ACHKNB_RS03655"
                     /old_locus_tag="pgaptmp_000731"
     CDS             complement(45449..45853)
                     /gene="ybgC"
                     /locus_tag="ACHKNB_RS03655"
                     /old_locus_tag="pgaptmp_000731"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4194298.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(46003..46296)
                     /gene="ybgE"
                     /locus_tag="ACHKNB_RS03660"
                     /old_locus_tag="pgaptmp_000732"
     CDS             complement(46003..46296)
                     /gene="ybgE"
                     /locus_tag="ACHKNB_RS03660"
                     /old_locus_tag="pgaptmp_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HGU4194299.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(46296..46409)
                     /gene="cydX"
                     /locus_tag="ACHKNB_RS03665"
                     /old_locus_tag="pgaptmp_000733"
     CDS             complement(46296..46409)
                     /gene="cydX"
                     /locus_tag="ACHKNB_RS03665"
                     /old_locus_tag="pgaptmp_000733"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HGU4194300.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(46424..47563)
                     /gene="cydB"
                     /locus_tag="ACHKNB_RS03670"
                     /old_locus_tag="pgaptmp_000734"
     CDS             complement(46424..47563)
                     /gene="cydB"
                     /locus_tag="ACHKNB_RS03670"
                     /old_locus_tag="pgaptmp_000734"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4194301.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(47579..49147)
                     /gene="cydA"
                     /locus_tag="ACHKNB_RS03675"
                     /old_locus_tag="pgaptmp_000735"
     CDS             complement(47579..49147)
                     /gene="cydA"
                     /locus_tag="ACHKNB_RS03675"
                     /old_locus_tag="pgaptmp_000735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HGU4194302.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT        12113 a        13194 c        12271 g        11883 t
ORIGIN      
        1 caaaatactt ttgtatttat ggatacggaa aacgcttccg ctcgccataa ggcgtatttt
       61 atacctggta cttgttaagt gtcgttttta gtaagaaaaa aaatcccgac acatggccgg
      121 gatattgatg gtgaaaaaga attaacggcg gttgccaaaa attcgcagca acatcaggaa
      181 caggttgatg aagtccagat acaaggttaa cgcgccaaga atggaatatt tgcgcaggtt
      241 cgacgtgtcg cgggtatcaa tctgctcacc catatttttc aatttctgag tgtcatacgc
      301 cgtcaatccg acaaagacaa tcacgccgat gtaggtaact gcccacatca atgcttcgct
      361 tttcagccag aagttgacca gcgatgccag cacaatgccg attaacgcca taaacagcat
      421 attgccaaag ccacttaaat cgcgcttcgt ggtgtaaccg tacaggctca ttgcgccgaa
      481 catcccggca gtaacgacga aagtactggc gatagaagca gcggtataga caatgaatat
      541 actggaaagc gtaagacccg tcagcgccga ataaagcata aagagcatcg tcgttacacc
      601 tgcgctcagc ttttgaatca tcgctgataa cacaataacc aatgctaatt gcgcgatgat
      661 cagaccgatt aaaaagacac ggttagtaaa caacagctcc atcacggccg cggaattagc
      721 cgcataccag gcaacaaatg cggtcagcaa caagccaacg gtcatccagc catagacttg
      781 cgccatataa gtttgcaagc cagcccgggg ttgtacgatt gaatcagaac gtgggaatct
      841 gtccatgacg atctcctgaa gatataagga atatcttaag gatactgcaa aatgatgagg
      901 ctgtgcatcg acgcagcgta aacgcatgta ctgagcggtg aaattgccgg acgcagcggt
      961 gccttatccg gctaacaaaa aactaccagc gttttgccgc ctgctgatcg ctctcccggg
     1021 cttcaaccca gcggtcgcct tccggcgtgg cttcgcgctt ccagaacggt gcgcgggttt
     1081 tgagataatc cataataaac tgcccggctt caaacgcact gctgcgatgc gcactggtga
     1141 caccgacaaa aacgatttca tcgcccggcc ataattcccc gatgcggtga atcaccgtga
     1201 cgcgccccag cggccagcgg ttacgcgctt catcaacaat ttctgccagt gctttttcag
     1261 tcatccccgg atagtgttcg agggttaatg ctttgacgct gtcgcccagg ttatggttgc
     1321 gcaccttacc ggtaaaggtg actaccgcac cgtcttcgtc acgctccgcc agccacgggt
     1381 actcttctcc tacgctgaac ggctgcgggc cgacaacaat tttggtttct gccatcttaa
     1441 cctccggtta ccggcgggaa gaaagctact tcgtcgccgt cagtcagcga atggtcaaaa
     1501 ctcaccagcg tctggttaac ggcagccagc aatttgccat cctccagcgc caacgcccag
     1561 cgatcgctct gcgcagccag gtgctggcgt aacgcttcaa cggttgggaa atccgcagcc
     1621 acttcggttg catctgttcc caccaactcg cgcacctggg cgaaaaaaag aactttaatc
     1681 atccgcttcc accttaaagt cacccgactt gccgccgctc ttcgccagca aacgtaccgg
     1741 accaatcacc atatcttttt gcaccgcttt gcacatgtca taaatggtca gcgccgccac
     1801 ggaggccgcg gttaatgctt ccatttcgac accggttttc ccggtcaggc ggcataaggt
     1861 ttctatacgc acccgattgt gctccggctc ggcctgtaaa ttgacttcaa ctttgctgag
     1921 catcagcgga tgacagagcg ggatcagatc ccaggtgcgt tttgccgcct gaataccggc
     1981 aatacgcgca gtggcaaata cgtcgccttt gtggtggcga ccatcaataa tcatcgccag
     2041 cgtctcgctg cgcatggtga caaaggcttc cgcccgcgct tcacgcacgg tttccgcttt
     2101 ggcggagaca tccaccatgt gcgcttcgcc agcggcgttg atatgggtca gttgcgacat
     2161 acttatttct tcaaatgtgg atggaaatta cacggacgcg tacgggcatc cagctgcggc
     2221 gcgatgatat tttcccatgc ggtacggcac gctttggtcg aacccggcat ggcgaaaatc
     2281 agcgttttgt tggcgacgcc cgctaccgca cgagattgca acgtggaagt gccaatctct
     2341 tcaaacgaca acatacggaa cacttcacca aaaccttcaa tttcacggtc gaacaacggc
     2401 agcaatgctt cgggagcctg atcaccttcc gtcaggccag taccacccgt aatcaatacc
     2461 acttgtacat cgtcgctggc gatccacgca gatacctgag cgcgaatagc gtagcggttt
     2521 tctttcacaa tggctttatc gacaacgtga tggcccgctt cttgcgccga atcgcgcaga
     2581 tagtgaccgg aggtatcgtc ttcttcaccg cgacgattag aaaccgtaag aatagcaata
     2641 cgggtcggga taaattcagt gcttacctga ctcatctgat ctctcctttt gacgttttag
     2701 ccgccaatgt acgataagtt ttgcgtaata ccggtgttgt tttgatgcag gaaatgggtc
     2761 tgtttcttct cccgcagcgc cgctgaaata cgcgcttcca gcgcctgttg ctgggtatcg
     2821 tcttccagca gatcgcgcag gttaacgccg ccttcaccaa acaggcagag atggagttta
     2881 ccaatggagg aaacgcgcag gcggttgcaa gtggcgcaga agtctttttc atacggcatg
     2941 ataaggccaa tctctccggc gtaatctgga tggcaaaaga cttgcgcggg accgtcgctg
     3001 cgttgacgta attggtggat ccagccgcga cgcagtagct cgtcacgcag aacctgacca
     3061 gagatgtgat gcttacggaa gagctcgctg ccctcgcccg tttccatcag ttcgatgaaa
     3121 cgcagctgga tagggcgatg ctggatccag ttcagaaagg tgtcgagctg gtgatgatta
     3181 acatcacgca tcagcacggt attgactttg accttctcaa aaccggcctc aaatgcagca
     3241 tcaatccccg ccatgacctg gttgaattta tcctgcccgg taatagcgtg aaactggcgg
     3301 gcgtccagac tgtcgacact gacgttaatg ccagtaagtc ccgcatcgcg ccagttcgcc
     3361 acatcgcgtt ccagacggta accattggtg gtgaccgcaa tctggcggat agcgtcgttt
     3421 tcccgcacag cggcgatgat atcggtaaag tcgcggcgta aagacggctc accgcctgtc
     3481 aggcgcactt tttcggtgcc cagactggcg aaggcgcgcg taacccggcg aatttcatcg
     3541 acggtaagaa agcctttatt ggtgacgccg ctcggtttgt agccatccgg caggcagtag
     3601 gtgcaacgaa agttacacac atcggtaatc gacaggcgca agtagtaaaa cttacgcgca
     3661 aatgcatcag tcagttgtga agccatgtac acctttccag atacgggagg cgaagtcatt
     3721 tcttccttcg ccctggtggc aatctttacg agcattgtca cggccaaagc accgtatcag
     3781 ttgacccagg tgcagaggct agagtgttta gtggttatgc cgatactagc gtgtaaatga
     3841 gatttttacc atccacactt tcgctatgta atcatgtata tagcgtcatg atcgtgctat
     3901 ttcgctacag aataagggaa aatggcggtt ttactttgat atacatcatc agggaggcat
     3961 agcgtgcatc gtcctttaga ggtagaagaa aatagtttgt atcctcaata ttggcaggtt
     4021 aattgctgtt tccccgcaat ttgcgctact gtagcgcgga taatctgact ccaggaaact
     4081 atatgcgcaa tcgtacgctg gctgatcttg atcgtgtcgt tgctctcggc ggagggcatg
     4141 gactgggacg cgttctctca tcactttcgt ctttgggttc tcgtttaacg ggtatcgtca
     4201 ccaccaccga taatggtggc tcgacggggc gtattcgccg ttcagaaggc ggcattgcct
     4261 ggggcgatat gcgcaactgc ctcaaccagc tgataacgga accgagcgtc gcctccgcga
     4321 tgtttgaata ccgttttggt ggcaatggcg aactttccgg tcataatctc ggaaacttga
     4381 tgttaaaggc gctggatcac cttagcgtgc ggcctctgga agccatcaat ttaattcgta
     4441 atctgctgaa agtggatacg catttgattc caatgtcaga gcatcctgtt gatctgatgg
     4501 cgattgacga tcaggggcat gaagtttacg gcgaggtcaa tatcgaccag ttaactacgc
     4561 cgattcaaga gttattgtta acgcctaatg tacccgcaac gcgtgaggcg gttcacgcta
     4621 tcaatgaagc ggatctcatc attattgggc ctggcagttt ttataccagc ctgatgccaa
     4681 ttctgctgct gaaggaaatc gcccaggcat tacgccgcac gccagcgccg atggtttata
     4741 tcggcaatct ggggcgtgag ttgagtttac ctgcggctaa tttgaagctg gaaagcaagc
     4801 tggcaattat ggagcagtat gttggtaaaa aggtcattga tgcggtcatc gtcgggccaa
     4861 aagtggatgt ctcggcggtg aaagagcgga ttgtgatcca ggaggtactg gaggccagcg
     4921 atattccgta tcgtcatgac cgccagttgt tacataacgc gctggaaaag gcgttacagg
     4981 ctttaggtta acggaaatcc gaaggaaaat tccggcttcc tattgaagac aaagtgcgcg
     5041 ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaccgcg caaattcaat
     5101 atattgcgga gaaaatgtag gcctgataag cgtagcgcat caggctgttt tccgtttgtc
     5161 atcagtcttc ttcgctatcc tgttacgatg ccgcgataaa cagctcacgc agctgatgca
     5221 actggtcacg aatttgcgcc gcttcttcga actccagatt ctgcgcgtgt tgcatcatca
     5281 acccttccag ctcatggatt ttctgctgca acgctttagg cgacatatcc atcggcacat
     5341 tatccggctc aacaatcggt cgcgattttc ctctgccctt cgctttggtt ttagcaatgt
     5401 tctgccccaa cgccaggata tcgaccactt tcttgttcaa gccttgcggc gtaatgccgt
     5461 gttcctcgtt gtacttctgc tgtttctcgc ggcgacgttc ggtttcgcca atcgctttcg
     5521 ccattgatgg ggtgatctta tcgccgtaga gaatcgcttt accgttaacg ttacgtgccg
     5581 cacgaccaat ggtctggatc aacgaacgtt cggaacgcag gaagccttct ttgtcagcgt
     5641 cgaggatcgc caccagcgaa acttccggca tatccagacc ttcacgcagt aagttgatcc
     5701 ctaccaatac gtcgaactca cccagacgca agtcgcggat aatctccata cgttcgacgg
     5761 tgtcgatatc tgagtgaaga taacgcacgc gctcgccatg ttcttcgaga tattcggtaa
     5821 gatcttccgc catccgcttg gtcagtgtgg tgaccagtac gcgttcgtta attgccgctc
     5881 gctgacgaat ctccgaaaga agatcatcaa cctgtgtcgc caccggccgc acttcgataa
     5941 tcgggtcaag caatccggtt ggacgcacca cctgatccac cacatcgccg ccggattttt
     6001 ccagctcgta attacccggc gtcgccgaaa catagatggt ttgcggcgct aatgcttcga
     6061 actcttcaaa cttaagcgga cggttatcca gcgctgatgg caggcggaag ccgtactcca
     6121 ccagtgtctc tttacgcgcc cggtcaccgc gatacatgcc gccaatttgt ggaatggtga
     6181 cgtgagattc atcgaccacc agcagcccat cagcagggag gtaatcaaac agcgtcggcg
     6241 gtggctcacc cggtccacga ccggagagga agcgcgagta gttttcaatc cccgaacagt
     6301 agcccagctc gttcatcatc tccagatcaa actgggtacg ctgggtcagc cgctgctctt
     6361 ccagcagttt gttgttttcc aacagcactt tgcgtctggc ggccagctct tctttgatct
     6421 cctccatcgc ctgtacgatg cgctcgcgcg gtgtgacgta gtgcgttttc gggtagatgg
     6481 taaaacgtgg aatagtggaa acaatctgcc cggtcagcgg gtcaaataac gacaatcgtt
     6541 ccacttcctc gtcaaacagt tccacgcgaa gtgcaatgtc atccgattct gccgggaaga
     6601 tatctatcac ctcgccacga acgcggaaag taccacgctg gaatgcttga tcattacgag
     6661 cgtattgcag ctccgccagt cggcgcagaa tcgcgcgctg atcgataatc ataccgaccg
     6721 tgagatggag catcatcttg agatataaat caggatcgcc cagaccataa atcgcggaaa
     6781 cagacgccac cacaaccaca tcacgccgct ccagcatcgc tttggtggcg gacaaacgca
     6841 tctgctcaat atgttcgtta accgaggcat ctttctcaat gaaagtgtcg gaactcggta
     6901 cataggcttc cggctgatag tagtcgtagt aggaaacgaa atattccacc gcgttttccg
     6961 ggaagaactc tttcatttcg ccatacagct gggccgccag cgttttgttg ggcgcaagta
     7021 ccatggttgg gcgctgaagg tcagcaatga cattggcaat ggtgaatgtt ttcccggagc
     7081 cagtcacgcc aagtaacgtc tggtgcgcca ggccatcttc cagcccctct tcgagacgtc
     7141 gaatcgcctc tggctgatcg ccagaaggtt taaaagcgga attcagtttg aacggtttac
     7201 tcatgagtcg ctacctgaag gagttgggcg ggcaggtatg taattttact cgtcgtactt
     7261 aattatgcca acaaattata ctggataaaa aaacagttca tcaccataat atttctgtta
     7321 cagcgtaaac tccgctgtca atcatgagca aaatttactc tgtggcgaga taaaactccg
     7381 gccttaccgg gttatcccca aagcaacggc ttttttaaca tttgtcaaga tgagtgatga
     7441 cagttttatg gcaagagatg cctgttcagt gactgccatt gcatttatct aaccagttaa
     7501 aaaataaaag atatttcttt gagccgtctt taacgccaat tcgcgtgcaa gccgcgtatt
     7561 ctctcgcttg cctcgtgttt tctaactcta atacacatgg ttatccacag gaatagtgga
     7621 taactgtctc cagcccctat cccccgccgc ctgggcaatt cccacattcg ccgcatgagg
     7681 cggaagtaaa atttttttcg tgattatttt agaatttaat tggttaaatt gcagtcaatc
     7741 gaagacgcga tctcgctcgc aatttaacca aatacaggat agctacaaca aggcaaggtt
     7801 tatgtacttt ccggttgccg cattttctgg attttctgca agccagggga tctctcccag
     7861 cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat tcagcgtgac gttttcccgg
     7921 aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt ccggcgtgtt gtattgcctg
     7981 cgcagtcaac atcgcgtgat taatacagcc gagtttcaca ccaactacca gtatcaccgg
     8041 cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg tcagaaagcg gcgtaaacca
     8101 gccgccagca ccttccacta acacccagtc agcctgttgt tcaagcgcgc gtaatccggc
     8161 gctcattacc aatgattcta tcggcctgcc ctcttgcgcg ctgatgatgt gcggcgaggt
     8221 gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc agctgcaggc tgctgttgtg
     8281 ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg gtcttttcgc tgccagaggc
     8341 gaccggttta taacctgccg tccgataacc tactgccttc gcggcttgta aaagtgcaca
     8401 actggcgacg gttttcccca cttcggtatc cgttccggtg acaaaataac gtttactcac
     8461 gagcaatcac tcccaaaaaa agatgatacg tcagaggata tcgcccctgt tgttgcggcc
     8521 aggccagttg caatcgctgc aattgcgaac gcgttaatat tcgcgggtcg cgcccttcat
     8581 gaagatgcgt agcaccgatg cctttcagcg aacgcatggc actgagcgca tcatcaaacc
     8641 atagtgtgat gggctgaata tgatgttgat aatgcacgcc gttcagcgac tgttcgattt
     8701 catctggcgg taaaaagcga ttagcatgcg gacgctcgtc caccgcctgc cacgcctgat
     8761 gcagttcggg taacgatccc tgctccagcg tggtaaacgc caccacgcct ccggggcgca
     8821 ccacccgata cagctcgcgg agtgccgtgg ataaattacc gcaccactgc actgcgagat
     8881 tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag ggattcgata tctcccgcca
     8941 gataatggtc tgcggcatcc ttctggcgtg cctgaacaag cattggcggc gagagatcta
     9001 aggccgtcac ctgcgcgtga cgttcccgcc agtggcggct catccagcca ggtccacaac
     9061 ccgcgtccag tacgtgggtg tatttacgct gtgggagcat tgccagtaag acgtcagcac
     9121 tctggcgctg tagatctgcg tgttgctcat agagtgcggc tgcccgacca aatgccgctg
     9181 caatggcttg tttattaacc gttgccatgc agcacctcca gcagacggtc gatatcctgc
     9241 atttcatgcg ctgcggttag cgttaagcgc agtcgcgcag taccagcggg tacggttggc
     9301 gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt tttctgccag ttgtaacgca
     9361 cggctgttat caccgacgat caatggctgg atggcgctgc atgaatcagc cagcgtaaac
     9421 ggcaaatcct gtactccggc acgaaaacgc gcaatgagtg acaccagttt ttcgcgccgt
     9481 gcatccccct catcacggcg aatgaccgcc agtgacgcac gtaatgcctg cgcctgagcg
     9541 ggcggcatac tggtgctgta gataaggtgg cgggcgaatt gcagcagata atccgccacc
     9601 gtacttgagc aaagcaccgc agccccactg acgccaaatc ctttgccaaa agtcactacc
     9661 agcaattctg gttttacctt ttgcagccag cagctgccgc gcccctgctc cccgataacg
     9721 cccgtgccgt gggcatcatc gaccatcaac cagccattgt gctgttgcgt tacctgctgg
     9781 atttccgcca gtggtgcact atcgccgtcc atgctgaaca cgccttctgt caccaccagt
     9841 tgctgccccg gacaggggga agcaagcaat cgcgccaaat gagtgacatc gttatgagca
     9901 aaacggcgaa gctgcgacgg gcttaaactg gcagcttcca gcaatgaggc atggctaagc
     9961 cggtcagcga caatgcggtc ctcttttgcc atcatcgcgg caatgaccgc ctgattagcg
    10021 gcaaaaccag agataaatag cagtgcccgc gaatagccca gccactcggc cagttcttct
    10081 tccagcgcct gatgcgcaac gctataaccg ctgacgtgac cggaaccgcc gctaccgacg
    10141 ccaaattgct ccgcaccctg ctgccaggca cggataattt gcggatgatg gcttaaacct
    10201 aaataatcgt tactggaaaa gttcagatac tggcgatcat ccgccaccag ccagcgtccg
    10261 gctccttgcg ccactggata acggcgacgc agggcatcgg cagcacgccg cgcatcgagc
    10321 gccgcgttga ttttctcctg ccagctcata gtgctgccgc gttgtaatat tcgtcagtgt
    10381 ccggggtcat cagtgcctgc tccagacgct gctgttgttc gttatcccct gccagcacgg
    10441 cagtttgctg cggatttagc cccagtttgt gaaacagttg caggtcttta tcttcttccg
    10501 gattcggcgt ggttagcagt ttgcaaccgt agaaaatcga gtttgcgcct gccataaagc
    10561 acatcgcctg agtctgttcg ttcatctgct cgcgtccggc agaaaggcgc acgtaagagg
    10621 tcggcatcat gatccgcgcg accgcaatgg tgcgaataaa atcaaaggca tcgacatcat
    10681 cgttatccgc cagcggcgtg cctttcacct tcaccagcat gttgattggt acgctttccg
    10741 gcggcgtcgg caggtttgcc agttgtagca ataatccggc gcgatctttt accgtttcgc
    10801 ctaagcccac aatgccgcca gaacagactt tgatcccggc atcgcgcact ttttccagcg
    10861 tatcgaggcg ttcctgataa gtgcgggtgg tgatgatatt gccgtaaaac tccggcgagg
    10921 tgtccaggtt gtggttgtag taatccagcc cggcgttcgc gaggcgctgc gcctgagatt
    10981 cactcaacgt gcccagcgtc atacacgcct ccagccccat cgcttttacc ccctgcacca
    11041 tttgttccag gtacggcata tcgcgttcgt ggggattctt ccacgccgcg cccatacaga
    11101 aacgcgtcga tcccgcagct ttcgctttgc gcgccgactc cagcacctgt tcaacttcca
    11161 tcaaccgctc ggcttccagc ccggttttgt agcgcgagct ttgcgggcag tatttgcaat
    11221 cttccggaca agctccggtc ttaatcgaca gcaaagtgct gacctgcacc tgacgaggat
    11281 cgaaatgctg tcgatgcacc tgctgcgctt caaacagcag atccagcaac ggtttttcaa
    11341 ataattctgt gacttgcgac aatgtccagc gtgggcggtg agccatgggg cttctccaaa
    11401 acgtgttttt tgttgttaat tcggtgtaga cttgtaaacc taaatctttt caatttggtt
    11461 tacaagccga ttatgacaac ggacgatctt gcctttgacc aacgccatat ctggcaccca
    11521 tacacatcca tgacctcccc tctgccggtt tatccggtcg cgagcgccga aggttgcgag
    11581 ctaattttgt ctgacggcag acgcctggtt gacggtatgt cgtcctggtg ggcggcgatc
    11641 cacggctata atcacccgca gcttaatgcg gcgatgaagt cgcaaattga tgctatgtcg
    11701 catgtgatgt ttggcggtat cacccatgcg cccgccatcg agctatgccg caaactggtg
    11761 gcaatgacgc cgcaaccgct ggaatgcgtt tttctcgcgg actccggctc cgtagcggtg
    11821 gaagtggcga tgaaaatggc gttgcagtac tggcaggcca aaggcgaagc gcgccagcgt
    11881 tttctgacct tccgcaatgg ttatcatggc gatacctttg gcgcgatgtc ggtgtgcgat
    11941 ccggacaact caatgcacag cctgtggaaa ggctatctgc cagaaaactt gtttgctccc
    12001 gccccgcaaa gccgcatgga tggtgaatgg gatgaacgtg atatggttgg ctttgcccgc
    12061 ctgatggcgg cgcatcgtca tgaaatcgcg gcggtgatca ttgaaccgat tgtccagggc
    12121 gcaggcggga tgcgcatgta ccatccggaa tggttaaaac gaatccgcaa aatgtgcgat
    12181 cgcgaaggta tcttgctgat tgccgacgag atcgccaccg gatttggtcg taccggcaaa
    12241 ctgtttgcct gtgaacatgc agaaatcgcg ccggacattt tgtgcctcgg taaagcctta
    12301 accggcggca caatgaccct ttccgccacg ctcaccacgc gcgaggttgc ggaaaccatc
    12361 agtaacggcg aagccggttg ctttatgcat gggccaactt ttatgggcaa tccgctggcc
    12421 tgcgcggcag caaacgccag cctggcgatt ctcgaatctg gcgactggca gcaacaggtg
    12481 gcggctattg aagtacagct gcgcgagcaa cttgcccccg cccgtgatgc cgaaatggtt
    12541 gccgatgtgc gcgtgctggg ggcaatcggc gtagtcgaaa ccactcatcc ggtgaatatg
    12601 gcggcgctgc aaaaattctt tgtcgaacag ggtgtctgga tccggccttt tggcaaactg
    12661 atttacctga tgccgcccta tattattctc ccgcaacagt tgcagcgtct gaccgcagcg
    12721 gttaaccgcg cggtacagga tgaaacattt ttttgccaat aacgagaagt ccgcgtgagg
    12781 gtttctggct acactttctg caaacaagaa aggagggttc atgaaactca tcagtaacga
    12841 tctgcgcgat ggcgataaat tgccgcatcg tcatgtcttt aacggcatgg gttacgatgg
    12901 cgataatatt tcaccgcatc tggcgtggga tgatgttcct gcgggaacga aaagttttgt
    12961 tgtcacctgc tacgacccgg atgcgccaac cggctccggc tggtggcact gggtagttgt
    13021 taacttaccc gctgataccc gcgtattacc gcaagggttt ggctctggtc tggtagcaat
    13081 gccagacggc gttttgcaga cgcgtaccga ctttggtaaa accgggtacg atggcgcagc
    13141 accgccgaaa ggcgaaactc atcgctacat ttttaccgtt cacgcgctgg atatagaacg
    13201 tattgatgtc gatgaaggtg ccagcggcgc gatggtcggg tttaacgttc atttccactc
    13261 tctggcaagc gcctcgatta ctgcgatgtt tagttaatca ctctgccaga tggcgcaatg
    13321 ccatctggta tcacttaaag gtattaaaaa caactttttg tctttttacc ttcccgtttc
    13381 gctcaagtta gtataaaaaa gcaggcttca acggattcat ttttctattt catagcccgg
    13441 agcaacctgt gaacacattt tcagtttccc gtctggcgct ggcattggct tttggcgtga
    13501 cgctgaccgc ctgtagctca accccgcccg atcaacgtcc ttctgatcaa accgcgcctg
    13561 gtacctcttc tcgcccgatt ctgtcggcaa aagaagcgca gaatttcgat gctcaacact
    13621 attttgcatc cctgacacca ggtgctgcag cgtggaatcc ttccccgatt accctgcctg
    13681 cgcaacctga ctttgttgtc ggcccggcgg gcactcaagg tgtaacgcat accacgattc
    13741 aggcggcggt agatgcggca attatcaagc gtaccaacaa gcgccagtat attgccgtga
    13801 tgcctggtga gtatcaggga acggtatatg tccctgccgc tccgggtgga attactctgt
    13861 acggtacagg tgaaaaaccg attgatgtga agattgggct ttcccttgat ggtggcatga
    13921 gccctgccga ctggcgtcac gacgtcaacc cgcgcggcaa atatatgcca ggtaaaccag
    13981 cgtggtatat gtacgatagc tgccagagca aacgcagcga cagtatcggt gttctctgct
    14041 ctgcggtctt ctggtcacaa aacaatggcc tgcaactgca aaatctgacc atcgaaaaca
    14101 cgctgggcga tagcgtagat gcaggtaacc atccggcggt ggcactgcgt actgatggtg
    14161 accaggtaca gattaacaac gttaacattc tcggtcgtca gaacaccttc tttgtcacca
    14221 acagcggtgt gcagaaccgt ctggaaacca accgtcagcc gcgtacgctg gtgaccaaca
    14281 gctacattga aggggatgtg gatatcgttt ctggtcgcgg cgcagtggtg ttcgataaca
    14341 ccgaattccg cgtggtgaac tcacgtactc agcaagaagc gtatgtgttt gcaccggcta
    14401 cgctgtccaa catttactac ggtttcctcg ccgtaaacag ccgtttcaat gctttcggtg
    14461 atggtgtggc gcaactgggc cgctcgctgg atgttgatgc caataccaac ggtcaggtgg
    14521 tgatccgtga tagcgccatc aacgaaggtt ttaacacggc taaaccgtgg gccgatgcgg
    14581 tgatctctaa tcgtccgttt gcgggtaata ccggcagcgt agatgataac gacgaaatac
    14641 agcgcaatct gaatgacact aactacaacc gcatgtggga atacaataac cgcggcgtgg
    14701 gtagtaaagt ggttgcagag gcgaagaagt aagagcaatt aactatttgc cggatgcggc
    14761 gtaaacgcct tatccggcct acggttcgat gcgatttgta ggtcggataa gatgcgcaag
    14821 catcgcatcc gacaataagt gccggatgct gcgaaaatgc cttatctggc ctacagattc
    14881 gatacgattc gtaggtcgga taagatgcgc aagcatcgca tccgacaata agtgccgaat
    14941 gcgacctaca ttcacatggc gctttttaca tctgacggtt tttattgaag ttaatcaaac
    15001 tacccgcctt gataatctcg cgctcttcag cagtcagact ttccatatag agcgtaattt
    15061 ccgttaccgg cgcatcttca tggatcacat aacctttaaa cgtcgtaccc ggattatcca
    15121 gcgccgcttt aatgccaggg atgtaaatgt aatcccccac ttcaaaggtt ggcacttccg
    15181 ccatctgcag cggtaacatc ccccagttga tgacgttaga acggtagcgt ttagtcgcgt
    15241 actcttcggc aatattcgcc agaccgccaa tcacacgctg gcagctcgcc gcctgttcac
    15301 gcgcagaacc atcgcctggt ttcaccgcat ataccatgct gccaatttca gtttgcagcg
    15361 gatcaatatg ctcctgacca gcaatctgct taatgcgcgc aaacacctct gtcagctcgc
    15421 tgacattccc cgccagacgc tgattttcca gctcagcagt cgctttactt ctgccaacat
    15481 aaccgggatc gcggcgtgac agggtaaact ccgccagacc aatcggattt gaacgataag
    15541 aagaggtttc accggaagga atcagttcgt cggtggtggt cacttcgtcg aggatcttcg
    15601 agcacacttt caggacgata ttgtcagtca gcgcacccaa ttccggccag tctttaatgt
    15661 tcggtccgta aatcagcggt tgctgagttg cccctttcac aaagccctga taaacacggt
    15721 ttttatacgg cgttacatcg aaggcgtact ccggcacgtt gtcccagcaa tcaagttcgc
    15781 tggcagaggt taaatagcca ccgtttgccg cagtcgcagc gatagaacga gcgtccatca
    15841 acgccaccgc tgacatctgc ccattagctg gcttagagcc ttcgcggttc gggaagttac
    15901 gcgtggtgtg gcgaatactc aaaccgttgt tgattggcgt atcgcccgcg ccaaagcatg
    15961 ggccgcagaa cgcggttctg atgattgcgc ctgcgccaat caaatctgct accacacctt
    16021 ttttggcgag atccataaac accggctgtg atgacgggta aactgccagc gagaaggtgt
    16081 cattgccaca ggattgaccg cgcagtgcat tcgccgccgc gatgacgttt tcgtagttac
    16141 cgccagaaca gcccgcgata atcccctgct gcactttcag gcgaccattt tccactttat
    16201 ccagcagcga gagtttggct ttaccgtgcg ccacgcgttc ggactcaatt tcaatctcac
    16261 gcagaatgtc ggtcaggttc tggttcagtg tgtcgatttt atacacgttg ctcgggtgga
    16321 acggcagcgc aatcattggt ttgatggcgc ttaaatcaac gctgatgcag ccatcgtagt
    16381 acgccatcgg ttgagggtta agctggcagt aatcctggcc gcgaccgtgc agcgccagcc
    16441 agttatgcac ttcttcatca gtttgccaga cggagcttaa acaggtggtt tccgtggtca
    16501 tcacgtcaac gctgttacgg aaatcggtag agagcgcgct aacgcccggt ccaacgaact
    16561 ccatgacttt gtttttgacg taaccgtttt tgaacaccgc gccaatgata gccagcgcca
    16621 catcctgtgg ccccacatac ggcgcaggtt ttccggtcag atgcaccgca accacgcccg
    16681 gatagtcgat atcccaggtg tcattaagca gctgttttac caactcaccg ccgccctcac
    16741 cgactgccat tgtccctaat gcaccgtaac gggtgtggct gtctgacccg aggatcattt
    16801 tgccgccgcc tgccatcatc tcacgcatat attgatggat gaccgcaata tgcggaggca
    16861 caaaaatacc gccataacgc tgggccgccg ataaaccaaa aacatggtca tcaccgttaa
    16921 tagtgccgcc gacggcgcag agtgagttat ggcagttggt cagcacatac ggcagcggga
    16981 aacgttccat accggacgct ttagcggtct gtacaatacc aacaaaggta atatcgtgag
    17041 aggctaatga atcaaactta attttaagtt tatccatatt tccggacgta ttatgcgaag
    17101 agagaataga ccaggcaata gtgccttttt tggcttcttc ttttttaatt tcgccggtga
    17161 aatgttcttc ggcaattatt tcgttattac tggcgagaaa cacgcctttt tcagataact
    17221 tgatcatcaa atgctccaga tatctgcgca ccgtgaagtg cgcagaaaga taataaatgg
    17281 atatactcta aataattcga gttgcaggaa ggcgacaagc gagtgaatcc ccaggagctt
    17341 acataagtaa gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga
    17401 cgagaatatt tacagcatgt tccagccgat cagcatattc caccaccaca cgccgagggt
    17461 gatatgcacc aggaaggtta atatcgtcag taccgcaccg accaaccacc aggatttaat
    17521 atcgttataa cccacgccaa agatgaccgg acctgccgcg ccgccatagt gagtaaccat
    17581 gccgccatag gagttggaga acaacagtgc cagcgcggtt aacattaacg gtgcgccgga
    17641 gacgttcgcc agcatggcaa ataccggtaa catagcaacg atataggcac taccggaagc
    17701 gaagaaataa cgcacgataa tgctgaggaa aataataacg aagaaagcaa cgttaccgtg
    17761 accatcaaat gccaggttat ttttaaagac ttcagctaac cattcgaaga atttaacttt
    17821 cgataataag gagcttaatc cgataatacc gccgtaccag attaaggtat tccagccgcc
    17881 tttattttta accacgtctt cccaggtaac gatacccagc agcagcatgg tcgccataac
    17941 aacgattgcc acagtggatt catcaacccc cagagactta ctgaaaatcc agcccagtag
    18001 cgccagcaca aagacaccga gcagcatttt ttcgcggatt ttcatcggtc ccagttcggc
    18061 aaggcccgct ttagcgatgg ttttattatc caccttctta atttctggcg gatacatggt
    18121 ataaatcacc agtggggtga ccagcagcat aatgatgccc ggcaatccgg cggccagtgc
    18181 ccatccaccc cagctaattt gcaggtgcag aatgtcgttg atcattttca gcgccagaat
    18241 gttccccgcc attgcggtaa agaacatata gctggtggtt ttggtgacca tgtaaatgga
    18301 catcatcagg taatgtccga cacgacgcgg acttttttcc ggttcggacc ccaaagccac
    18361 cgccacgctg ttgatgatcg gtaacacaat accgcccgca cgcgcggtgt tagacggtgt
    18421 tgccggagcc agtaccagat cgaggaatac cgtaacgtaa cccagaccca gcgtggtgtt
    18481 accgatttta ccaatcagca gataggcaat acgtttacct aaaccggtgg tcacaaatgc
    18541 ggcgcttaag gtaaacgccg agaacaccag ccaggtagta cctgaagagt aaccgcttaa
    18601 tacggcggtg gtcttaaacg caccgtcgga taagttaccg accaccacca tggaggcagc
    18661 aacggcaatt aacagtacga caggttccgg gaaaggcttg ataaccagcc ccacaatggc
    18721 cgccaggtaa ataccaaaaa gcacccacgc cagttcgctt aatcctgccg gagctggcat
    18781 aaaaccaata atacatggga tcgctaatat cagaattagc ttccataacg atttcttatt
    18841 catttaaaat cctgttcttt ctgtatgact catacagaag tcctttattg attatcctta
    18901 tcttgacaac gaattttttc agggaagata aacttcaccg gaaaatattt ttctggtcgt
    18961 ccgaataaca gatgcgcgta acgtcgtggc atcctgacct tcattactta aatgaacgtc
    19021 gagcgcacca ctggggtgtt caatattgat attgccgtat cctacagaag ggacgatttg
    19081 tcgggtgacg gtgccttcca atgcacaact actggaaata gcaatagcac cggttatcgc
    19141 cagcgcgcga tggcaagaat gcggcataaa ataacgcaca ttaattgccc cgcctttctg
    19201 cgctggagaa ataagcacag gtttagggat aaccatatta ctgacatcac ctaagcccat
    19261 tgctttaccc gcttgtagac ggatagattc aatgcgggct aataatgctt tgtcggcatc
    19321 cagttccgcc ggtaattcat aacctgtttt tcccagatat tcagccggaa taatgacgac
    19381 tggcatcgcc atatcgatac aggtcaccgg gacatcgtca aaataatcaa tctgattatc
    19441 agtcgggaaa acttttccgg ttttggttcc agcggcattc aggaaagtga gcgcaaccgg
    19501 tgcggcagta cccggtacgc cgtcaattct ggcactaccc tcgtactcga caacaccatt
    19561 tggcgtttgc acatcagctt cgatgaacgt acccgtattg acgttgcgga tacgcacacg
    19621 ggtgaccggt gatgtcgctg caatcaaacc attttcaatg gcgaatgccc caacaccaga
    19681 cagcatattg ccacaattag gcgtggtatc gacacgttgc tcatggacaa taacttgagc
    19741 aaacagataa tcgacatcag cacgcggatc gctggaacgg ctaataatgg cgactttact
    19801 ggtcagcgga ttaccgccgc caataccgtc aatttccaga tcgttaccgg aacccataat
    19861 tgccatcaat attttatcgc gctgcgtttg atcttcgggt aaatgttccg ctaacaagaa
    19921 cgcgcccctc gaggttccac ctcgcatcat cacgcagggt attttttcat aataatgccc
    19981 tcaatttaaa aacataactt attgcgatat ggttacatta aggacaaagc atctctaatg
    20041 actttagctc aacgattaat aacatttttt cattaattta agttggctaa atgcagttaa
    20101 aatttttaac gaccagccac ccaaaatcgt cgcttccatt ttccggagta ataatgaagc
    20161 atgaattatc aagtatgaag gcatttgtca tactggcaga gtccagttca tttaacaatg
    20221 ccgctaaatt actcaatatt acgcaacctg cattaacgcg aagaataaaa aagatggagg
    20281 aagatttaca tattcaacta tttgagcgta caactcgcaa ggttacgtta acaaaagcag
    20341 gaaaaaggtt gctcccggag gcgcgggaat taataaagaa attcgatgaa acgcttttta
    20401 atattcgtga tatgaatgct tatcatcgtg gtatggtgac gttagcgtgt attccgaccg
    20461 cagtatttta ttttttaccg ctggcaatcg gtaaatttaa tgagctgtat cccaatatta
    20521 aagtgcggat tctggaacaa ggcacgaata attgcatgga atcggtgctg tgtaacgaat
    20581 cagactttgg cataaacatg aacaacgtca caaattcatc cattgatttt accccactgg
    20641 tcaacgagcc attcgtgctg gcctgtcggc gtgaccatcc attggccaaa aagcagcttg
    20701 tagaatggca ggagttagtg ggttacaaaa tgattggcgt acgttcctcc agcggcaacc
    20761 gattgttgat agaacaacag ctagccgaca agccctggaa gctggactgg ttttacgaag
    20821 tgcgtcatct ttcgacgtca ttaggactag tggaggcagg actggggatt tcggcgcttc
    20881 ctggtctggc aatgccccat gcgccctatt cttcgattat tggtatccca ctggtggaac
    20941 ccgttatacg tcggacattg gggattattc gccgcaaaga tgccgtgctt tctccggcag
    21001 ctgaacgctt ttttgccttg ctgattaatt tgtggactga cgataaagac aatttgtgga
    21061 ccaatatcgt cgaacgccag agacatgcgt tacaggagat aggttaatac accggcgcag
    21121 gagagactgc gccggttaaa tcagcggtta gtgtgcgtta accaccaccc acattggtcc
    21181 ctgcccgacc gcataacggc ctttttcatg cagtagtccc tgctcgccaa caatttcgta
    21241 taccgagatg tggtgagatt tttgcccggc ggcaatcaga tacttgccgc tgtgatcaac
    21301 attgaagccg cgcggctggg tttccgttgg ctggaagcct tctttactca acacgctgcc
    21361 atcttccgaa acgctgaaaa cggtaatcag gctggcggta cggtcgcagg cgtataaatg
    21421 gcgaccatcc ggggtgatat gaatatcagc cgcccaacgg gtgtcggaga agttttccgg
    21481 catcatatcc agcgtctgga cacattcgat attaccgtgc ggatctttca gttcccagac
    21541 atccactgag ctgtttaact cattgacgca atacgcatat tgttcgtttg gatggaatac
    21601 catatgacgc gggccggccc cttcaacggt ggtcacttcc gcagggtcct gcgccacgag
    21661 atgaccatca tcgctgaccg taaacaggca aatgcgatcc tgctttaatg ccggaaccca
    21721 cagcgtacgg ttgtccggtg agatattggc ggaatggcaa ccgtccagcc cctcgaccac
    21781 atcgacgacg cccactggca ggccatcttc cagacgcgtt acgctcacgt tacccgcatt
    21841 gtaagaacct acaaagacaa actgcccctg gtgatcggtg gaaatatgcg tcggactacc
    21901 cggcagcgca gactctgcgg caaaggtcag tgcgccatcg tccggggcga tacgatacgc
    21961 caggacgcga aactcagggc gaacaccaac atagagataa cgtttgtccg ggctgaccac
    22021 catcggctgc acctgccccg gcacatcgac aacctgtgtc agcgtcagtg cgccttcatg
    22081 attcaggttc cagacgtgaa tttgctggct ctcagggctg gcgatataaa ctgtttgctt
    22141 catgaatgct cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc
    22201 ggtgaatgct gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc
    22261 gcagacgaaa attcttaacc ttaatctgga atacgccatg accacacgcg tgattgctct
    22321 cgacttagac ggcaccttat tgaccccgaa aaagaccctg cttccttcat cgatagaagc
    22381 cctggcccgc gctcgcgaag caggctatca attaatcatc gtcacaggtc gccatcacgt
    22441 cgctattcat cctttttatc aggcgctggc gctggataca cctgctattt gctgtaatgg
    22501 cacctatttg tatgattatc atgcaaaaac cgtgctggaa gcggacccaa tgcccgttaa
    22561 taaagccctg caactcattg agatgctgaa tgaacaccac attcacggtc tgatgtatgt
    22621 ggatgatgca atggtctatg agcacccgac cgggcatgtc attcgcacgt ctaactgggc
    22681 gcaaaccctg ccgccggaac agcgtccgac tttcacacaa gtcgcttctc tggctgaaac
    22741 ggcgcaacaa gttaacgccg tatggaagtt cgccctgacg cacgatgacc tgccgcaatt
    22801 gcagcatttt ggtaagcatg tcgaacatga actgggactg gagtgtgaat ggtcctggca
    22861 cgatcaggtt gatattgcac gcggcggcaa cagcaaaggt aaacgtttga cgaaatgggt
    22921 tgaggcgcaa ggctggtcga tggaaaacgt cgtggcgttc ggcgataact ttaatgatat
    22981 cagtatgctg gaggccgctg gtacaggcgt ggcgatgggc aacgccgatg acgcggtaaa
    23041 agcgcgcgcc aacattgtga ttggtgataa caccaccgac agcattgccc agttcattta
    23101 tagccacctg atttaatcag gcggttatcg acacactttt aatttgcgcg tacagccaca
    23161 ggccaggttt gatcaccagt tcatccctgg cccacgggct gatacgcgcc cacagcgttt
    23221 tacctccgac ttccagttcc acttccacct ggccgttgtc gtcataacta ttaacaactt
    23281 ttgcccgcaa tacgttacga atgctggttt gctgcggcgg ttgtaaaacc agagaaacat
    23341 cggaagcctg aatgcggata cgtagcgcag cttgcagcgg ttcgtccagc ttattgaccc
    23401 acaaatgctg atcgcccagc gccagcgcag tcatcgcgta atgcggatga tgctcaagta
    23461 ccgtcacttt cagaatgcta ctttgttgct ctttcggcag ccacggattc atcacgctac
    23521 taccccagac ttcttccagt gcgccaaagg ctttcacctg accgttttcc agtaccatca
    23581 ctctgtctgc cagatggagg atctcatcca gcgaatggct gacatacaac atcgggatgt
    23641 tgatttcccg cgtcagacgt tgcagataag gcaacagttc gcgtttacgc ggaatatcca
    23701 gtgacgccag cggttcatcc agcaacagca attctggtgc cgtcagcaaa gcccgcccaa
    23761 tcgctacgcg ctgtttttcg cctccggaca ggctgcctgg taaacggtca agcaacggtt
    23821 caatgcctaa aagcgccacc agcttatcga actgatcgac catgcttttc gccatgccgt
    23881 agcgcagatt gccacgcact ttgtaatgcg gaaacagccg cgcatcctga aaaacatagc
    23941 caacgcgacg cttttctggc gtcaggcaga tacctttttc ggcatcattt agtacccgac
    24001 cattgaggac aatcctccct ttttgcgggc gcgtcagtcc actgatggcg ttaatcagcg
    24061 aagtttttcc ggcaccggag acgccaaaga tagcagtgat gccattggcg ggcagcgttt
    24121 cattaatggt caggcaatgg ttgcccaagg tctgggaaaa attcagttcc agcatgatta
    24181 gcgccccgcc cgttcacggc tgattctggc cagccattct gaaatcaaca gggagatcat
    24241 cgccagcgca atagaaataa tgcacagtct cgccgcgcca ctttccccgc cgggggtctg
    24301 gatcagggta tacatggcag aaggaatggt tcgcgtttca ccaggaatgt tcgacacaaa
    24361 ggtgatggtt gcgccaaatt caccgagaga acgagcaaaa gccagtaccg taccaacaat
    24421 aattcccggt aaggtcagcg gtatcgtgat agtaaagaaa acgcgccagc gcccggcccc
    24481 cagtgttctt gcggcctgtt ccagtttgac atcaacccct tccagcgcca gacgaattgc
    24541 ccgcaccatc agcggaaacg acatgacggc ggcagcgaga accgcgccgc gccagctaaa
    24601 ggcgaaggta atgccaaacc agtcatacag acgttcaccg ataaacccgc gccgtcccat
    24661 cgaaactaat aataagtaac cgacgaccac gggcggtaac accagcggta gatgcagtac
    24721 gctgtcgagc agagctttgc ccggaaacgt gcaacgcacc agtaaccagg caaaaaagat
    24781 cccaaacggc aggctaaaca gcacagccag ggaagaaact ttcaggctta ataaaactgc
    24841 ctgccattct ggatcggtca gtatcattac ttggttgtaa atccgtaacg tttaaagatt
    24901 tccgcagcct gcggcccttt caaataatca taaaatgctt tcactgtcgc attgttatgc
    24961 ccttccacaa cagcaaccgg gtattccact tttttatgcg aatcttccgg gaaggtggcg
    25021 accactttta cccctttgct ggcaactgcg tcagaaccgt aaactatgcc cagcggcgct
    25081 tcattgcgtt cgaccaacgc cagcgcgcca cgaacatctt ccgccggagc cagtttcgga
    25141 gagagcgtat cccatgcgcc cagtttttgc agtgcttctt ttgcataaat gccagcggga
    25201 acatgttccg gatcgccaac cgccagacga ccgccattca gcagcgaagt ccagttggtt
    25261 ttgctgtcga tggtgaaatc tttctgctcg ctggctttcg gtgctacaac gaccaggcta
    25321 ttgccgagca gtgtctgacg cgtagcagta tcgatcgctt ttttatcaac tgcataatcc
    25381 atccatttct gatcggcaga aataaacaga tccgcaggcg cacccgcttc aatctgacgg
    25441 gcgagagtag atgacgaagc gaaagaagaa accacatcca cgcctttttc ttttttatac
    25501 tgcgtagcaa tgtcctgcat tgcgttagtc agtgatgcgg cggcgaacac cgtgattttc
    25561 ccttcatctg ccagtgtatt gccagcaaca gcgaaagaga gtgccgcccc ggcaaacaag
    25621 ttcaaccatt tacgagccat tggtaacccc ttaatgtaac gttatatagg tgacaatata
    25681 acggctagct cagaattttc ccagcagtta ttgctaacct ttgtgaggta gataagaaaa
    25741 aatatcggca ggaaaagcag gaagttgaga aaaagaaaat gcccgactaa gcgggcattc
    25801 agggaatcaa tgattttgtc cgggctggtc ttttttacca acaccagaaa agatgttgaa
    25861 tacttcacca agaccgtaaa tcagacccag gatgatggcc atcacgacag gtaccatgat
    25921 tacggcgaat accagacttt ttaataactc taacatggtc aactccagat atagtcatga
    25981 gactattcta accgctaagc acagaaaagc actccccttt tgtgcggtca gctttgcgtg
    26041 gcgttcgttt tccgtcacaa taagactttt gccaggacat tgttatgcag gccgaaatcc
    26101 ttctcaccct taagctccaa caaaaattat tcgccgaccc gcgccggatt tcgctactaa
    26161 aacacattgc gctttccggt tccattagcc agggagcgaa agatgccggt atcagctata
    26221 aaagcgcctg ggatgccatt aacgagatga atcagttaag tgagcatatt ctggtcgagc
    26281 gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac ccgctatggt cagcgactga
    26341 ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc ctttgatgtg ttaagtgacg
    26401 atgacgccct gccgctgaac agcctgctgg ccgcgatctc acgtttttca ctgcaaacca
    26461 gcgcccgtaa ccagtggttc ggcaccatca ccgcccgcga tcatgatgac gttcaacagc
    26521 atgttgatgt cttactggct gacggaaaaa cacgcctgaa agtcgcaatt accgcacaaa
    26581 gcggcgcgcg tctggggctg gatgaaggca aagaagtgtt gatattgcta aaagcgccgt
    26641 gggtaggtat tactcaggac gaggcggtcg cgcaaaacgc tgacaaccaa ttaccgggta
    26701 ttattagtca tattgagcgc ggcgcagagc agtgcgaagt attaatggcg ctacccgacg
    26761 ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac ttctcttcag caagggcaga
    26821 atgtcacggc ctactttaat gccgacagcg tgattatcgc cacgctgtgc taagcgtgtt
    26881 gacaatttgt tatgaaacac gtatccctgt cagtaatcgc tgcacaaaat gggatataaa
    26941 atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta gcgacacaaa aacgctgcaa
    27001 ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt ttgtcggttc gaatggaagc
    27061 gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc cgcttttgaa aggtgaacgg
    27121 caaagccagt tttcccacat cactcgtctc tccttcgagc aattgcaaaa actcgtcagc
    27181 gacgaatggc agcggaataa caccgatatg ctcggccctg gcgaagatga caccggacgc
    27241 actacggctg agatcattca ggatgaagta aaggatgcac cgcgttgcat gcaactggcg
    27301 cagcagttcg gtattaccgc cctcctcgac cgacgcttta aatacctttc cactggcgag
    27361 acgcgaaaaa gcctgctgtg tcaggcgctg atgtcggagc ctgacttgtt gattcttgat
    27421 gagccgttcg atggcctgga tgttgcctca cgtcagcagc tggctgagcg actcgcctcg
    27481 ttacatcagt ccggtattac tctggtactg gtgctcaatc gcttcgatga gatcccggag
    27541 tttgtccagt ttgctggcgt gctggcggat tgcacgttag cggaaactgg cgctaaagag
    27601 gaactgctcc aacaagcact cgtcgcgcaa ctggcgcata gtgaacagct tgaaggtgtg
    27661 caactgccgg agccggatga accttcagca cgtcacgcct tacccgccaa cgaaccgcgc
    27721 attgtgctga acaatggcgt ggtttcttat aacgatcgcc ccattcttaa taaccttagc
    27781 tggcaggtga atccaggcga acactggcaa attgtcgggc caaatggtgc aggaaaatcg
    27841 acgttattaa gcctggttac tggcgatcat ccgcaaggtt acagcaacga tttgacgctt
    27901 ttcggacgac gtcgcggcag cggcgaaacc atctgggata tcaaaaagca tatcggttac
    27961 gtcagcagta gtttgcatct ggattaccgg gtcagtacta ctgtgcgtaa tgtgattctt
    28021 tctggctatt ttgattcgat tggcatttat caggccgttt cggatcgcca gcaaaaactg
    28081 gtgcagcagt ggctggatat tctcggcatt gataaacgca cggctgacgc tcctttccat
    28141 agtctttcct ggggacagca gcgtctggcg ctgatcgtcc gcgcactggt gaaacatccg
    28201 acgttgctta ttctcgatga accactacag gggcttgatc cgctcaatcg ccagcttatc
    28261 cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc aattgttgtt tgtttcgcac
    28321 cacgctgaag atgcgcctgc ctgtattacc catcgccttg agttcgtgcc ggacggtgga
    28381 ctctatcgct atgtgctgac aaaaatatat taagtcggta gtgctgacct tgccggaggc
    28441 ggcctaagcg ccctctccgg ccaacggttc gacgcatgca ggcatgaaac cgcttctttt
    28501 ttcagataaa aagcgcaatc attcataaac cctctgtttt ataatcactt aatcgcgcat
    28561 aaaaaacggc taaattcttg tgtaaacgat tccactaatt tattccatgt cacacttttc
    28621 gcatctttgt tatgctatgg ttatttcata ccataagcct aatggagcga attatgagag
    28681 ttctggttac cggtggtagc ggttacattg gaagtcatac ctgcgtgcaa ttactgcaaa
    28741 acggtcatga tgtcatcatt cttgataacc tctgtaacag taagcgcagc gtactgcctg
    28801 ttatcgagcg tttaggcggc aaacatccaa cgtttgttga aggcgatatt cgtaacgaag
    28861 cgttgatgac cgagatactg cacgatcacg ctatcgacac cgtgatccac ttcgccgggc
    28921 tgaaagccgt cggcgaatcg gtacaaaaac cgctggaata ttacgacaac aatgtcaacg
    28981 gcactctgcg cctgattagc gccatgcgcg ccgctaacgt caaaaacttt atttttagct
    29041 cctccgctac cgtttatggc gatcagccca aaattccata cgttgaaagc ttcccgaccg
    29101 gcacaccgca aagcccttat ggcaaaagca aattgatggt ggaacagatc ctcaccgacc
    29161 tgcaaaaagc ccagccggac tggagcattg ccctgctgcg ctacttcaac ccggttggcg
    29221 cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat tccgaataac ctgatgccat
    29281 acatcgccca ggttgctgta ggccgtcgcg actcgctggc gatttttggt aacgattatc
    29341 cgaccgaaga tggtactggc gtacgcgatt acatccacgt aatggatctg gcggacggtc
    29401 acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt acacatctac aacctcggtg
    29461 ctggcgtagg cagcagcgtg ctggacgtgg ttaatgcctt cagcaaagcc tgcggcaaac
    29521 cggttaacta tcattttgca ccgcgtcgcg aaggcgacct tccggcctac tgggcggacg
    29581 ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg cacactcgat gaaatggcgc
    29641 aggacacctg gcactggcag tcacgccatc cacagggata tcccgattaa ggaacgacca
    29701 tgacgcaatt taatcccgtt gatcatccac atcgccgcta caacccgctc accgggcaat
    29761 ggattctggt ttcaccgcac cgcgctaagc gcccctggca gggggcgcag gaaacgccag
    29821 ccaaacaggt gttacctgcg cacgatccag attgcttcct ctgcgcaggt aatgtgcggg
    29881 tgacaggcga taaaaacccc gattacaccg ggacttacgt tttcactaat gactttgcgg
    29941 ctttgatgtc tgacacgcca gatgcgccag aaagtcacga tcctttgatg cgttgccaga
    30001 gcgcgcgcgg caccagccgg gtgatctgct tttcaccgga tcacagtaaa acgctgccag
    30061 agctcagcgt tgcagcattg acggaaatcg tcaaaacctg gcaggagcaa accgcagaac
    30121 tggggaaaac gtacccatgg gtgcaggttt ttgaaaacaa aggcgctgcg atgggctgct
    30181 ctaacccgca tccgcacggt cagatttggg caaatagctt cctgcctaac gaagctgagc
    30241 gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa atcaccaatg ctggtggatt
    30301 atgttcagcg cgagctggca gacggtagcc gtaccgttgt cgaaaccgaa cactggttag
    30361 ccgtcgtgcc ttactgggct gcctggccgt tcgaaacgct actgctgccc aaagcccacg
    30421 ttttgcggat caccgatttg accgacgccc agcgcagcga tttggcgctg gcgttgaaaa
    30481 agctgaccag tcgttatgac aacctcttcc agtgctcctt cccctactct atgggctggc
    30541 acggcgcacc atttaatggc gaagagaatc aacactggca gctgcacgcg cacttttatc
    30601 cgcctctgct gcgctccgcc accgtacgta aatttatggt tggttatgaa atgctggcag
    30661 agacccagcg agacctgacc gcagaacagg cagcagagcg tttgcgcgca gtcagcgata
    30721 tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa agaaaaaaca caatctctgt
    30781 ttgccaacgc atttggctac cctgccactc acaccattca ggcgcctggc cgcgtgaatt
    30841 tgattggtga acacaccgac tacaacgacg gtttcgttct gccctgcgcg attgattatc
    30901 aaaccgtgat cagctgtgcg ccacgcgatg accgtaaagt tcgcgtaatg gcagccgatt
    30961 atgaaaatca gctcgacgag ttttccctcg atgcgcccat tgtcgcgcat gaaaactatc
    31021 aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca actgcgtaac aacagcttcg
    31081 gcggcgtgga catggtgatc agcggcaatg tgccgcaggg tgccgggtta agttcttccg
    31141 cttcactgga agtcgcggtc ggaaccgtat tgcagcagct ttatcatctg ccgctggacg
    31201 gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca gtttgttggc tgtaactgcg
    31261 ggatcatgga tcagctaatt tccgcactcg gcaagaaaga tcatgccttg ctgattgact
    31321 gccgctcact ggggactaaa gcagtttcca tgccgaaagg tgtggctgtc gtcatcatca
    31381 acagtaactt caaacgtacc ctggttggca gcgaatacaa cacccgtcgt gaacagtgcg
    31441 aaaccggtgc gcgtttcttc cagcagccag ctctgcgcga tgtcaccatt gaagagttca
    31501 acgctgttgc acatgagctg gacccaatcg tggcgaaacg cgtgcggcat atcctgactg
    31561 aaaacgcccg caccgttgaa gctgccagcg cgctggagca aggcgacctg aaacgtatgg
    31621 gcgagttgat ggcggaatct catgcctcta tgcgcgatga tttcgaaatc accgtgccgc
    31681 aaattgacac tctggtagaa atcgtcaaag ctgtgattgg cgacaaaggt ggcgtacgca
    31741 tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat cccggaagag ctggtgcctg
    31801 ccgtacagca agctgtcgct gaacaatatg aagcaaaaac aggtattaaa gagacttttt
    31861 acgtttgtaa accatcacaa ggagcaggac agtgctgaac gaaactcccg cactggcacc
    31921 cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac gcagggatgg tagtcacgct
    31981 gatggactgg ggtgcgactt tactttcagc tcgtattccg ctttccgatg gcagcgtccg
    32041 cgaggcgctg ctcggctgtg ccagcccgga atgctatcag gatcaggctg cgtttctggg
    32101 ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc cgttatacct ttgacggtga
    32161 aaccgtgacg ctttcgccaa gtcagggcgt taaccagctg cacggcggcc cggaagggtt
    32221 cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt caggtgctgt ttgccctgag
    32281 ttcagatgat ggcgatcagg gcttcccggg taatctcggc gcgacggtgc aatatcgtct
    32341 gaccgacgat aaccgtatct ctattactta tcgcgccaca gttgataaac cttgcccggt
    32401 gaatatgact aatcacgtct atttcaatct tgacggcgag cagtctgacg tgcgcaatca
    32461 caagttgcag attctggcgg acgaatatct gccggttgat gaaggcggca ttccgcacga
    32521 cggcctgaaa tctgtcgccg gaacgtcttt tgatttccgt agcgccaaaa tcatcgccag
    32581 tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt tacgatcacg cattcttgtt
    32641 acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc tggtcagcag atgaaaaatt
    32701 gcagctgaag gtctacacca ccgctccggc tctgcaattc tactccggca acttcctcgg
    32761 cggcacgcca tcaagaggaa ctgaacctta cgccgactgg caaggcctgg cactggaaag
    32821 cgagtttctg ccggacagcc caaaccaccc tgaatggccg caaccggact gcttcctgcg
    32881 tcctggcgaa gagtattcca gcctgacgga atatcagttt attgctgagt agcacctgaa
    32941 gccctcgtaa tgagggcttt atccttatat tgctcaaatc accagcaaac accgacatat
    33001 ttgcaactca atattcacaa caaccttaca ctgcgacact attttcgcta tggttatgcg
    33061 taagcattgc tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaaaatga
    33121 tttgaggagt aagtatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca
    33181 gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt
    33241 aagcgaagca aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc
    33301 ttacacctct gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga
    33361 tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt
    33421 gcagggtctg aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg
    33481 gcgtcgtggt tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg
    33541 tcacgatccg cgttacgcga aactgagcga aaaagaactg ccgctgacgg aaagcctggc
    33601 gctgaccatt gaccgcgtga tcccttactg gaatgaaact attctgccgc gtatgaagag
    33661 cggtgagcgc gtgatcatcg ctgcacacgg taactcttta cgtgcgctgg tgaaatatct
    33721 tgataacatg agcgaagaag agattcttga gcttaatatc ccgactggcg tgccgctggt
    33781 gtatgagttc gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga
    33841 gatcgcagcg aaagcagcgg cggttgcaaa ccagggtaaa gcgaagtaaa cgtcattcgt
    33901 ttaaaatgag aaagccgact gcaagtgagt cggcattttt gttgctaaca atggagcaca
    33961 ttgcctgatg cgacgctgcg cgacttatca ggcctgtggt gattcatcgg atacgccact
    34021 ctgacggcgc atccgacaat taaaccttac ccgcgacgcg cttttactgc attcgccagt
    34081 tgacgtaaca gagcatcggt atcttcccag ccgatgcagg catcggtgat gctcttaccg
    34141 taggccagcg gctccccgct ctcgaggctc tgattgcctt ccaccagatg gctttccacc
    34201 atcacgccaa taatggcctt ttcgccaccg gcaatctgct ggcaaacgtc agcacaaaca
    34261 tccatctgct ttttgaattg tttggacgag ttagcatggc tgaaatcgat catcacctgt
    34321 gctggcaggc ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag
    34381 ttaggctctt taccgccgcg cagaatgata tggcaatcgc cgttaccgct ggtattcaca
    34441 atcgccgaat gcccccattt cgttacggac aggaagcagt gcggcgcacc ggcggcatta
    34501 atggcatcga tagccacttt aatcgtaccg tcggtgccat ttttgaagcc gaccggacaa
    34561 gaaagccctg atgccagttc gcggtgcacc tgcgattcgg tggtacgtgc gccaattgcg
    34621 ccccagctca tcaggtcagc gagatattgt ggggtgatca tatcgagaaa ctcacctgcc
    34681 gctggcagac cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg
    34741 ttgatctgga agctattatc catatgcgga tcgttaatca gccctttcca gcccaccgtg
    34801 gtacgcggct tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca
    34861 cgcagcgcca gcaagcgagt ggcatactct tttgccgcga caggatcatg aattgagcat
    34921 gggccaatca caaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt
    34981 cgggcatggg caaccgtatt cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg
    35041 acaggaggaa gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg
    35101 tctgttccag tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc
    35161 gtcagaatgt gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa
    35221 aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt
    35281 gtttgttaat tatttcaaga ttctctgctg cgtttcataa cctggctgaa atcttaaacc
    35341 aatgccttat attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat
    35401 aacaggagca tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa
    35461 gccttgccag cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg
    35521 caaacgccgc agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa
    35581 ataacgtcga caataacggg gtaaataccg gttctggtgg cacaatgctg cattcggatg
    35641 gttcttcaat gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca
    35701 aagatggtcg ctgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg
    35761 atgtcgatac caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga
    35821 gagcggagtc tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta
    35881 agtgagaata attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca
    35941 cctgccagaa gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt
    36001 tatgctggta gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc
    36061 gggtcatatg ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc
    36121 acgtcgtcct cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc
    36181 gtttgtgaat gccatagccc tcgtggtaat taccattttg attgtctggg aggcgataga
    36241 acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg atggcaattg ccgtggctgg
    36301 gttgctggca aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct
    36361 caacgtacga gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat
    36421 tattgccgcg ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat
    36481 actggtgtcg cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga
    36541 attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg
    36601 ggaaatcccg gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaatcc
    36661 ggtgatgacg ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca
    36721 gatccaacac tacctgatgg atcactatca gattgagcac gccaccattc agatggaata
    36781 tcaaccttgt catgggccag actgccacct gaatgaaggg gtgtctggcc attcacatca
    36841 tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg
    36901 gctgccgttg agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc
    36961 ctgaagtgca aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc
    37021 cacatactta cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc
    37081 ccagaacggg aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg
    37141 catgatcatg actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat
    37201 cagaccaatc gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca
    37261 gcgaattttc aactccgcct cgttctgact ggtttgtcgc gaccacgcat accaaccgta
    37321 aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa
    37381 gaaaataatg ccaaacaagg tgacgttgat caggccaaag aagtagttgc tgatcttctc
    37441 cagactggca aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga
    37501 gagatcataa ccgcctgccc ctattggtat atgtaccagg atattctgca cactaaaaaa
    37561 atccatcttt cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag
    37621 ccgcaaaatc cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctttcatcaa
    37681 catgaacctc gtgattgctt ccttcctgtt ctaatgcctc tgcgatggac tgaaggccat
    37741 tcatggccat ccacggacaa tgcgcgcagc tgcggcaggt tgcaccctca cctgcggttg
    37801 gtgcttccag taactcttta tctggcactg cctgctgcat tttgtagaaa atgccccgat
    37861 cggttgccac gataagccgc tgatgtggca atgttttcgc agcagcgatc agttgactgg
    37921 tggaaccgac cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta
    37981 tggcagcatc cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat
    38041 gcacaataca ggcaccctgc cagcatagaa tgtctccacc cgtctgtttt tgcacgtaac
    38101 gccccagatg tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa
    38161 taagttcgac ggcaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag
    38221 aagtgttggc gtagacgacg acagtacgat cgggatgggc atcgcaaaat gcgttaaatt
    38281 cttcaacagg gcagccgaga tccagtgaac attcagcctg aagtgtcggc atcagaattg
    38341 ttttttccgg actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca
    38401 aagtagaagc gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc
    38461 caccggtttc ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca
    38521 ccgcattacg ttcttttagc agacgtttta tcttctcgcg gtaatacgct ttttcatcaa
    38581 tgcttaacgg cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta
    38641 cgctcatctt accatctcgt tttacaggct taacgttaaa accgacatta gcgtaatatt
    38701 cgctgttatc ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa
    38761 gtcactcgaa ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg
    38821 atgtcatcag gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag
    38881 ggcgcttttt tacattggtg ggtcgtgcag gatgactcgc ttcgctcgcc cttcgggccg
    38941 tcgccgcgag cggctccgtt gtttcgctgc gctcaactca aacctgctgc aggttcgaat
    39001 ctttgagtat cgcagatagc aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc
    39061 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    39121 acgacccctt gcgggattat gctgctttaa tcattcagga tggtgggtcg tgcaggattc
    39181 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccatac
    39241 gggtgttacc tgaaagattt ctactcgggg ccaatcttta agattggtgt gtcgtgcagg
    39301 atgactcgct tcgcttgccc tacgggccgt tgctggcgca acgttatcct tcacgcttaa
    39361 catctgagtt tgatgttaaa attggtgggt cgtgcaggat tcgaacctgc gaccaattga
    39421 ttaaaagtca actgctctac caactgagct aacgacccga gtggtgggtg atgacgggat
    39481 cgaaccgccg accccctcct tgtaagggag gtgctctccc agctgagcta atcacccgat
    39541 actgcgctgg atactacaaa ctacttccac cgaacccacc gtaattagtg gtgggtcgtg
    39601 caggatgact cgcttcgctc gccctacggg ccgttgctga cgcaacgtta tcctacacgc
    39661 ttaacatctg tcttcgatgc taaaactggt gggtcgtgca ggattcgaac ctgcgaccaa
    39721 ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccggtggtgg gtgatgacgg
    39781 gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc
    39841 aatactacgc tggatactac aactaactat gttagtggtg ggtcgtgcag gatgactcga
    39901 cttcgtctcg cccttcgggc cgttgctgat gcaacgttat ccttcacgtt ttactaccgc
    39961 ttccacctta gcgattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag
    40021 tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg tttgtttgat
    40081 atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac aaaaatttcg
    40141 atgcagatca ctcaactgct tatgattcgc acgacacgac cagaaataat gcgacttctg
    40201 gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgt gcctgtttag cgccatcagt
    40261 accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg tgtcaccttt
    40321 gtcctgcatg atgacgccga ctttaaacat cgcatcagca gcctttggtg acttcggata
    40381 gtttttcact accgaagcaa aatagtacgc cgcatcatct tttttaccct tgttgtagtt
    40441 taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt aatttttgat
    40501 gaaattctga aatgccacca ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat
    40561 agctgcattg taatccgtgt ttgcatcgcc gcttttcacc ggcgcgccag cattcgcagt
    40621 accagcatca gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcgc tgctggttga
    40681 ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc aacaggatct gcttttgccg
    40741 ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccacgca gggaatcaat
    40801 atcggattga ttatcagaaa gttgttgctg gagttgggtt aaaagctggc tgtgagcatt
    40861 agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga cactactgat
    40921 tggtgcctga gcaaaagcgg cccagggggc cgctatacca accagtaacg acagactcaa
    40981 tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta ccgcacgacg
    41041 gtttttggag tatgccgctt cgtcatgacc cagtactgca ggtttttctt taccgtaaga
    41101 aacgatggag atctggtctg cagaaacgcc tttaccctgc aggtacatct taacggcgtt
    41161 cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt ccgcgtgacc
    41221 ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcatgtg catccagcat
    41281 ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga tgttgttctg
    41341 ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc
    41401 gttcgcatcc ataccagtgc cggcacccag catgccttcg ctgccgtcat tgctggcgtt
    41461 cttgttggaa gaacatgccg caattgccat aacaggcaga gcaatcatca gccctttcag
    41521 cactttgttc agttgcattt caatgattcc tttactattc aattaattat tatcacagat
    41581 acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc gctttgaaac
    41641 gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag ctgtagatta
    41701 ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac gacagaactt
    41761 gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccacca ttggagctga
    41821 ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc gaaccttccc
    41881 aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgc ggacgaccgg
    41941 cctggtcaga agtaaatgcc aggttctggc tatccgggaa ccaggtcggt tcggtattgt
    42001 tactgcgacc atcagtcacc tggcggatct gaccagaagc caaatccatt acgtacaggt
    42061 tcagactacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc gagaatgcag
    42121 gtgcaccgtt gtgacgcggg aatgaagcca cctgacgtac agcgccattt gccagcgtct
    42181 gaataaccag cgcggaacga ccgctttcga aggtcacata agccagttta gaaccgtctg
    42241 gtgaccacgc cggagacatc agcggctgcg gtgaacggtg aacgacaaac tggttgtaac
    42301 cgtcatagtc agatacgcgc agttcatacg ggaactgacc gccgttggtc tgaacaacgt
    42361 aggcaatacg agtacggaac gcacctttaa tgccggtcag tttttcaaac acttcatcac
    42421 tggcggtatg accagcataa cgcagccact gcttgttcac tttgtacgag ttctgagcaa
    42481 gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcaacattg taagaaccat
    42541 ccggattcgg agtgacctga ccgacaacta cagcgtcaat gcccagtgcg gaccatgcag
    42601 ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcgcga tctaacggat
    42661 taaatttacc gctgttacgc aagtcagcag caacgatgcc gccaatatct tcaggtgccg
    42721 caccaggccc cgcccactgg aaaggaacaa caccaatagg acgaccggaa tctacaccgc
    42781 tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg agaaaaccaa
    42841 atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg gcccacgata
    42901 atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg tggcaaccgg
    42961 tgcctgatgt tgaccgtccg aacagtcaac atcgcgatta cggtttgaag tccaatggcg
    43021 cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc gcaagtttag
    43081 ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg atatccagta
    43141 acataccatc gggtgccagt tttatgcgca gcgtacaggt tttgcctgca taggacgatg
    43201 cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta ttgatatcgg
    43261 cccctgatgc gccattgttt ttagtattac cactcccggc aggcgaagca ttgttccctt
    43321 tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg aaaatatcat
    43381 ctgcctctgc ggccgctttt gctgcagcgg ctttttcagc tgcagctttt gccgctgccg
    43441 cttttttgtc tgcggcggct ttttctgccg ctgctttctt atcagctgct gccttttcag
    43501 cagccgcttt ttctgctgct tttttgtcgg ctgcagcttt ctctgctgcc gctttcttat
    43561 cagctgcagc cttttcagca gccgctttct tctcagcttc tgctttggct ttttcagcag
    43621 cttcagttgc cgctttcttt cttgcttcag ctgcagctgc ttctgccgct tccgctttct
    43681 tcttcagtgc cgcagcggct gcctcggctt ttttctgcgc ttcggctgcg gctttggcgg
    43741 cttctgcttc tgcttttttc tttgcgtctg cagccgcttt cttcgctgct tcttccgcag
    43801 ctttagcatc tgcttcggcc ttcgctttag catctgccgc cgctttcgct gccgcctctt
    43861 cagcttgctt ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct
    43921 cctgagccgc taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt
    43981 tctcacgcag ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct
    44041 tcgcgcttga ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa
    44101 ccatgacagc gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat
    44161 cgaacgaact ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta
    44221 tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac
    44281 tgttcgcctg ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc
    44341 gtcattaaac caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt
    44401 tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg
    44461 aaacggctgg acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc
    44521 tcaaccacca cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg
    44581 ttactgctca ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg
    44641 atgatgggcg ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacggt
    44701 acaatgttga tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct
    44761 taccccttgt tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct
    44821 tccataaagt tgtcgtaatt cagttccagt ttgtttacgc gctggttgag gcggttataa
    44881 gccattaccg ccggaattgc ggcgaacaga ccaattgcag tcgcaatcaa cgcttctgcg
    44941 ataccgggcg caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg
    45001 tgcatgatcc cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta
    45061 ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata
    45121 gcacgcgacg ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc
    45181 acaaactctt tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc
    45241 ccctggctct cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac
    45301 gcttcggctt cgcgcgccgc tgcgttaaga atacgggtcc gctggataat aatggcccaa
    45361 gatgcgattg aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc
    45421 aggaacaaat caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg
    45481 gaagcgcacg aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat
    45541 tcagcaaagt attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca
    45601 ttgatgttat ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt
    45661 ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga
    45721 agtgatgatg acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt
    45781 ggtacaccac accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca
    45841 gcgttgtatt cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct
    45901 actatacgcg cgcgattgag gtttgggaat gagggcaagt taagggagcg taaaaattta
    45961 tgggcctgga gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat
    46021 cagcccgaca atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac
    46081 cttctgcgga cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa
    46141 taaaccatgc cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa
    46201 catacatcct gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc
    46261 cattaccgca tacaaagtcg cgataatctt actcatcaga tgtcttcttg accggctttg
    46321 cctgattcga cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc
    46381 gttcccagaa tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg
    46441 tgttacgttc aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg
    46501 tgtagagcag aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca
    46561 gctggctgga agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca
    46621 tcacaaacgg gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg
    46681 agaacacaaa cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcggca
    46741 gaaccacacc cagcgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc
    46801 atgcgccagc ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca
    46861 ttgtcgattt cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga
    46921 aacagaccag tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt
    46981 cgcccacggt acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca
    47041 ccacgcctgc cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca
    47101 gacgcagata ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac
    47161 caattaccag cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc
    47221 agcgggtttc ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg
    47281 ccagcacgag gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc
    47341 aggcagcaaa gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg
    47401 gtgcaatgga gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga
    47461 gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa
    47521 ccagcagcca ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt
    47581 agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc
    47641 tgcttgggcc gaggcgtgca aatttgaaca ttaagaacaa ttctgccacc aggaacaggg
    47701 tatacaggcc gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgagt
    47761 tcgccacagc tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca
    47821 cgaaccagcc cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca
    47881 gaagccattt tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga
    47941 ttgccagcag caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta
    48001 cacgcgggat ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt
    48061 ttggcgtata gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact
    48121 ggtcacgaac cgcctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct
    48181 tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg
    48241 gggtatccac ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt
    48301 tgttcgtctc ctcttcctga tcaggaatgc cgaacagagt aaaggcagca ggcgcaggtt
    48361 gcgtttccca ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt
    48421 agccggattc atcacccaga acaataacag acagaacagc agccataccg aagctggcag
    48481 cgatagcaaa ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc
    48541 tgataccgag gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt
    48601 tcacctgagc aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag
    48661 tttcaaagtt gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc
    48721 acagtgcgga caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt
    48781 tacccagacg atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg
    48841 ccatcagacc ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt
    48901 aagaccagtt agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga
    48961 agttgatacc aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg
    49021 agaggacgta gaccgtttcc ataatggcca gcaggaacgc cataccgagc gtcagtggca
    49081 caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat
    49141 ctaacatcat gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag
    49201 ggtcacacgc atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc
    49261 acacaaaaac ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca
    49321 cgtatatatt acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca
    49381 tttttcgacg gtgatcaatt tatagctaaa ttaccgcctt tcagccaatt tgatcgagaa
    49441 caatttatct ctttttgatg c
//
LOCUS       DAZFVO020000014        48414 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFVO020000014 DAZFVO020000000
VERSION     DAZFVO020000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 48414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.014"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2697
                     /gene="cas3"
                     /locus_tag="ACHKNB_RS03680"
                     /old_locus_tag="pgaptmp_000736"
     CDS             <1..2697
                     /gene="cas3"
                     /locus_tag="ACHKNB_RS03680"
                     /old_locus_tag="pgaptmp_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HGU4194303.1"
                     /translation="RKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDV
                     AAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPE
                     LKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTG
                     HHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWY
                     ISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGIN
                     TLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG
                     LFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVG
                     SEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKI
                     LLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQ
                     EAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQG
                     KCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYC
                     SLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDG
                     KDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMP
                     HAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYL
                     PEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLE
                     GEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2795..4357
                     /gene="casA"
                     /locus_tag="ACHKNB_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /gene_synonym="cse1"
     CDS             2795..4357
                     /gene="casA"
                     /locus_tag="ACHKNB_RS03685"
                     /old_locus_tag="pgaptmp_000737"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HGU4194304.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            4354..4887
                     /gene="casB"
                     /locus_tag="ACHKNB_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /gene_synonym="cse2"
     CDS             4354..4887
                     /gene="casB"
                     /locus_tag="ACHKNB_RS03690"
                     /old_locus_tag="pgaptmp_000738"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HGU4194305.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            4902..5975
                     /gene="cas7e"
                     /locus_tag="ACHKNB_RS03695"
                     /old_locus_tag="pgaptmp_000739"
     CDS             4902..5975
                     /gene="cas7e"
                     /locus_tag="ACHKNB_RS03695"
                     /old_locus_tag="pgaptmp_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HGU4194306.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPEFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            5986..6732
                     /gene="cas5e"
                     /locus_tag="ACHKNB_RS03700"
                     /old_locus_tag="pgaptmp_000740"
     CDS             5986..6732
                     /gene="cas5e"
                     /locus_tag="ACHKNB_RS03700"
                     /old_locus_tag="pgaptmp_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HGU4194307.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6714..7364
                     /gene="cas6e"
                     /locus_tag="ACHKNB_RS03705"
                     /old_locus_tag="pgaptmp_000741"
     CDS             6714..7364
                     /gene="cas6e"
                     /locus_tag="ACHKNB_RS03705"
                     /old_locus_tag="pgaptmp_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HGU4194308.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7361..8281
                     /gene="cas1e"
                     /locus_tag="ACHKNB_RS03710"
                     /old_locus_tag="pgaptmp_000742"
     CDS             7361..8281
                     /gene="cas1e"
                     /locus_tag="ACHKNB_RS03710"
                     /old_locus_tag="pgaptmp_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HGU4194309.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8281..8574
                     /gene="cas2e"
                     /locus_tag="ACHKNB_RS03715"
                     /old_locus_tag="pgaptmp_000743"
     CDS             8281..8574
                     /gene="cas2e"
                     /locus_tag="ACHKNB_RS03715"
                     /old_locus_tag="pgaptmp_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HGU4194310.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8671..9065
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8671..8699
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(9147..10184)
                     /gene="iap"
                     /locus_tag="ACHKNB_RS03720"
                     /old_locus_tag="pgaptmp_000744"
     CDS             complement(9147..10184)
                     /gene="iap"
                     /locus_tag="ACHKNB_RS03720"
                     /old_locus_tag="pgaptmp_000744"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HGU4194311.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10436..11344
                     /gene="cysD"
                     /locus_tag="ACHKNB_RS03725"
                     /old_locus_tag="pgaptmp_000745"
     CDS             10436..11344
                     /gene="cysD"
                     /locus_tag="ACHKNB_RS03725"
                     /old_locus_tag="pgaptmp_000745"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HGU4194312.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11346..12773
                     /gene="cysN"
                     /locus_tag="ACHKNB_RS03730"
                     /old_locus_tag="pgaptmp_000746"
     CDS             11346..12773
                     /gene="cysN"
                     /locus_tag="ACHKNB_RS03730"
                     /old_locus_tag="pgaptmp_000746"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HGU4194313.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12773..13378
                     /gene="cysC"
                     /locus_tag="ACHKNB_RS03735"
                     /old_locus_tag="pgaptmp_000747"
     CDS             12773..13378
                     /gene="cysC"
                     /locus_tag="ACHKNB_RS03735"
                     /old_locus_tag="pgaptmp_000747"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HGU4194314.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13428..13751
                     /gene="ygbE"
                     /locus_tag="ACHKNB_RS03740"
                     /old_locus_tag="pgaptmp_000748"
     CDS             13428..13751
                     /gene="ygbE"
                     /locus_tag="ACHKNB_RS03740"
                     /old_locus_tag="pgaptmp_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HGU4194315.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13945..14256
                     /gene="ftsB"
                     /locus_tag="ACHKNB_RS03745"
                     /old_locus_tag="pgaptmp_000749"
     CDS             13945..14256
                     /gene="ftsB"
                     /locus_tag="ACHKNB_RS03745"
                     /old_locus_tag="pgaptmp_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HGU4194316.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14275..14985
                     /gene="ispD"
                     /locus_tag="ACHKNB_RS03750"
                     /old_locus_tag="pgaptmp_000750"
     CDS             14275..14985
                     /gene="ispD"
                     /locus_tag="ACHKNB_RS03750"
                     /old_locus_tag="pgaptmp_000750"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGU4194317.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14985..15464
                     /gene="ispF"
                     /locus_tag="ACHKNB_RS03755"
                     /old_locus_tag="pgaptmp_000751"
     CDS             14985..15464
                     /gene="ispF"
                     /locus_tag="ACHKNB_RS03755"
                     /old_locus_tag="pgaptmp_000751"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HGU4194318.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15461..16510
                     /gene="truD"
                     /locus_tag="ACHKNB_RS03760"
                     /old_locus_tag="pgaptmp_000752"
     CDS             15461..16510
                     /gene="truD"
                     /locus_tag="ACHKNB_RS03760"
                     /old_locus_tag="pgaptmp_000752"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HGU4194319.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16491..17252
                     /gene="surE"
                     /locus_tag="ACHKNB_RS03765"
                     /old_locus_tag="pgaptmp_000753"
     CDS             16491..17252
                     /gene="surE"
                     /locus_tag="ACHKNB_RS03765"
                     /old_locus_tag="pgaptmp_000753"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HGU4194320.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17246..17872
                     /gene="pcm"
                     /locus_tag="ACHKNB_RS03770"
                     /old_locus_tag="pgaptmp_000754"
     CDS             17246..17872
                     /gene="pcm"
                     /locus_tag="ACHKNB_RS03770"
                     /old_locus_tag="pgaptmp_000754"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HGU4194321.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18057..19151
                     /gene="nlpD"
                     /locus_tag="ACHKNB_RS03775"
                     /old_locus_tag="pgaptmp_000755"
     CDS             18057..19151
                     /gene="nlpD"
                     /locus_tag="ACHKNB_RS03775"
                     /old_locus_tag="pgaptmp_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HGU4194322.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19214..20206
                     /gene="rpoS"
                     /locus_tag="ACHKNB_RS03780"
                     /old_locus_tag="pgaptmp_000756"
     CDS             19214..20206
                     /gene="rpoS"
                     /locus_tag="ACHKNB_RS03780"
                     /old_locus_tag="pgaptmp_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HGU4194323.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20327..20734)
                     /locus_tag="ACHKNB_RS03785"
                     /old_locus_tag="pgaptmp_000757"
     CDS             complement(20327..20734)
                     /locus_tag="ACHKNB_RS03785"
                     /old_locus_tag="pgaptmp_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGU4194324.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            20881..21474
                     /locus_tag="ACHKNB_RS03790"
                     /old_locus_tag="pgaptmp_000758"
     CDS             20881..21474
                     /locus_tag="ACHKNB_RS03790"
                     /old_locus_tag="pgaptmp_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HGU4194325.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21474..22901
                     /locus_tag="ACHKNB_RS03795"
                     /old_locus_tag="pgaptmp_000759"
     CDS             21474..22901
                     /locus_tag="ACHKNB_RS03795"
                     /old_locus_tag="pgaptmp_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HGU4194326.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFCLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            22912..23148
                     /locus_tag="ACHKNB_RS03800"
                     /old_locus_tag="pgaptmp_000760"
     CDS             22912..23148
                     /locus_tag="ACHKNB_RS03800"
                     /old_locus_tag="pgaptmp_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HGU4194327.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(23187..24551)
                     /gene="ygbN"
                     /locus_tag="ACHKNB_RS03805"
                     /old_locus_tag="pgaptmp_000761"
     CDS             complement(23187..24551)
                     /gene="ygbN"
                     /locus_tag="ACHKNB_RS03805"
                     /old_locus_tag="pgaptmp_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HGU4194328.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(24640..25416)
                     /gene="ygbM"
                     /locus_tag="ACHKNB_RS03810"
                     /old_locus_tag="pgaptmp_000762"
     CDS             complement(24640..25416)
                     /gene="ygbM"
                     /locus_tag="ACHKNB_RS03810"
                     /old_locus_tag="pgaptmp_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HGU4194329.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(25421..26059)
                     /gene="ygbL"
                     /locus_tag="ACHKNB_RS03815"
                     /old_locus_tag="pgaptmp_000763"
     CDS             complement(25421..26059)
                     /gene="ygbL"
                     /locus_tag="ACHKNB_RS03815"
                     /old_locus_tag="pgaptmp_000763"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4194330.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(26056..27318)
                     /gene="otnK"
                     /locus_tag="ACHKNB_RS03820"
                     /old_locus_tag="pgaptmp_000764"
     CDS             complement(26056..27318)
                     /gene="otnK"
                     /locus_tag="ACHKNB_RS03820"
                     /old_locus_tag="pgaptmp_000764"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HGU4194331.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(27315..28223)
                     /gene="ltnD"
                     /locus_tag="ACHKNB_RS03825"
                     /old_locus_tag="pgaptmp_000765"
     CDS             complement(27315..28223)
                     /gene="ltnD"
                     /locus_tag="ACHKNB_RS03825"
                     /old_locus_tag="pgaptmp_000765"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HGU4194332.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            28419..29186
                     /gene="ygbI"
                     /locus_tag="ACHKNB_RS03830"
                     /old_locus_tag="pgaptmp_000766"
     CDS             28419..29186
                     /gene="ygbI"
                     /locus_tag="ACHKNB_RS03830"
                     /old_locus_tag="pgaptmp_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HGU4194333.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASLALAKQDLSLLVAKNE"
     gene            complement(29237..29893)
                     /gene="pphB"
                     /locus_tag="ACHKNB_RS03835"
                     /old_locus_tag="pgaptmp_000767"
     CDS             complement(29237..29893)
                     /gene="pphB"
                     /locus_tag="ACHKNB_RS03835"
                     /old_locus_tag="pgaptmp_000767"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4194334.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(29999..32560)
                     /gene="mutS"
                     /locus_tag="ACHKNB_RS03840"
                     /old_locus_tag="pgaptmp_000768"
     CDS             complement(29999..32560)
                     /gene="mutS"
                     /locus_tag="ACHKNB_RS03840"
                     /old_locus_tag="pgaptmp_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGU4194335.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHSDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32636..32815
                     /locus_tag="ACHKNB_RS03845"
                     /old_locus_tag="pgaptmp_000769"
     CDS             32636..32815
                     /locus_tag="ACHKNB_RS03845"
                     /old_locus_tag="pgaptmp_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194336.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            32847..33200
                     /gene="ygbA"
                     /locus_tag="ACHKNB_RS03850"
                     /old_locus_tag="pgaptmp_000770"
     CDS             32847..33200
                     /gene="ygbA"
                     /locus_tag="ACHKNB_RS03850"
                     /old_locus_tag="pgaptmp_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HGU4194337.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(33237..35315)
                     /gene="flhA"
                     /locus_tag="ACHKNB_RS03855"
                     /old_locus_tag="pgaptmp_000771"
     CDS             complement(33237..35315)
                     /gene="flhA"
                     /locus_tag="ACHKNB_RS03855"
                     /old_locus_tag="pgaptmp_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HGU4194338.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(35389..36399)
                     /gene="hypE"
                     /locus_tag="ACHKNB_RS03860"
                     /old_locus_tag="pgaptmp_000772"
     CDS             complement(35389..36399)
                     /gene="hypE"
                     /locus_tag="ACHKNB_RS03860"
                     /old_locus_tag="pgaptmp_000772"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HGU4194339.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLSMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDMLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(36396..37517)
                     /gene="hypD"
                     /locus_tag="ACHKNB_RS03865"
                     /old_locus_tag="pgaptmp_000773"
     CDS             complement(36396..37517)
                     /gene="hypD"
                     /locus_tag="ACHKNB_RS03865"
                     /old_locus_tag="pgaptmp_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HGU4194340.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(37517..37789)
                     /gene="hypC"
                     /locus_tag="ACHKNB_RS03870"
                     /old_locus_tag="pgaptmp_000774"
     CDS             complement(37517..37789)
                     /gene="hypC"
                     /locus_tag="ACHKNB_RS03870"
                     /old_locus_tag="pgaptmp_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HGU4194341.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(37780..38652)
                     /gene="hypB"
                     /locus_tag="ACHKNB_RS03875"
                     /old_locus_tag="pgaptmp_000775"
     CDS             complement(37780..38652)
                     /gene="hypB"
                     /locus_tag="ACHKNB_RS03875"
                     /old_locus_tag="pgaptmp_000775"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HGU4194342.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(38656..39006)
                     /gene="hypA"
                     /locus_tag="ACHKNB_RS03880"
                     /old_locus_tag="pgaptmp_000776"
     CDS             complement(38656..39006)
                     /gene="hypA"
                     /locus_tag="ACHKNB_RS03880"
                     /old_locus_tag="pgaptmp_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4194343.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            39218..39679
                     /gene="hycA"
                     /locus_tag="ACHKNB_RS03885"
                     /old_locus_tag="pgaptmp_000777"
     CDS             39218..39679
                     /gene="hycA"
                     /locus_tag="ACHKNB_RS03885"
                     /old_locus_tag="pgaptmp_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HGU4194344.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            39804..40415
                     /gene="hycB"
                     /locus_tag="ACHKNB_RS03890"
                     /old_locus_tag="pgaptmp_000778"
     CDS             39804..40415
                     /gene="hycB"
                     /locus_tag="ACHKNB_RS03890"
                     /old_locus_tag="pgaptmp_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HGU4194345.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            40412..42238
                     /gene="hycC"
                     /locus_tag="ACHKNB_RS03895"
                     /old_locus_tag="pgaptmp_000779"
     CDS             40412..42238
                     /gene="hycC"
                     /locus_tag="ACHKNB_RS03895"
                     /old_locus_tag="pgaptmp_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HGU4194346.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            42241..43164
                     /gene="hycD"
                     /locus_tag="ACHKNB_RS03900"
                     /old_locus_tag="pgaptmp_000780"
     CDS             42241..43164
                     /gene="hycD"
                     /locus_tag="ACHKNB_RS03900"
                     /old_locus_tag="pgaptmp_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HGU4194347.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            43182..44891
                     /gene="hycE"
                     /locus_tag="ACHKNB_RS03905"
                     /old_locus_tag="pgaptmp_000781"
     CDS             43182..44891
                     /gene="hycE"
                     /locus_tag="ACHKNB_RS03905"
                     /old_locus_tag="pgaptmp_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HGU4194348.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            44901..45443
                     /gene="hycF"
                     /locus_tag="ACHKNB_RS03910"
                     /old_locus_tag="pgaptmp_000782"
     CDS             44901..45443
                     /gene="hycF"
                     /locus_tag="ACHKNB_RS03910"
                     /old_locus_tag="pgaptmp_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HGU4194349.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            45443..46210
                     /gene="hycG"
                     /locus_tag="ACHKNB_RS03915"
                     /old_locus_tag="pgaptmp_000783"
     CDS             45443..46210
                     /gene="hycG"
                     /locus_tag="ACHKNB_RS03915"
                     /old_locus_tag="pgaptmp_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HGU4194350.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            46207..46617
                     /gene="hycH"
                     /locus_tag="ACHKNB_RS03920"
                     /old_locus_tag="pgaptmp_000784"
     CDS             46207..46617
                     /gene="hycH"
                     /locus_tag="ACHKNB_RS03920"
                     /old_locus_tag="pgaptmp_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HGU4194351.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46610..47080
                     /gene="hycI"
                     /locus_tag="ACHKNB_RS03925"
                     /old_locus_tag="pgaptmp_000785"
     CDS             46610..47080
                     /gene="hycI"
                     /locus_tag="ACHKNB_RS03925"
                     /old_locus_tag="pgaptmp_000785"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HGU4194352.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            47123..47878
                     /locus_tag="ACHKNB_RS03930"
                     /old_locus_tag="pgaptmp_000786"
     CDS             47123..47878
                     /locus_tag="ACHKNB_RS03930"
                     /old_locus_tag="pgaptmp_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194353.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            47949..48368
                     /locus_tag="ACHKNB_RS03935"
                     /old_locus_tag="pgaptmp_000787"
     CDS             47949..48368
                     /locus_tag="ACHKNB_RS03935"
                     /old_locus_tag="pgaptmp_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4194354.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
BASE COUNT        11325 a        12371 c        13215 g        11503 t
ORIGIN      
        1 cgtaaatatc ctttaagttt actgaaggat aaaaatattg tgactttttt tgatttctgg
       61 ggaaaaaccc gacgcggcga gaaagacggg ggcgacgact atcaccttct ttgctggcat
      121 tcgctggatg tagccgcgat gggctattta atggttaaaa gaaattgctt cgggctggct
      181 gattattttc gtcaattagg gatttctgac aaggaacagg cggctcaatt tttcgcctgg
      241 ttgctgtgct ggcacgatat tggaaaattt gcccgctctt ttcaacaact ttacctgccc
      301 cctgaactca agattcagga aggtgccaga aaaaattacg aaaaaatctc tcattcaacg
      361 ctgggctact ggttgtggaa tcattattta agtgaatgtc aggagttact tccttcatct
      421 tcgctctctc cgcgcaaact taggcgagtg atagaaatgt ggatgcccgt gacgacaggg
      481 catcatggtc ggccccctga tcgtatggat gaattggata attttctgcc tgaagacaaa
      541 gctgcagcac gagattttct ccttgaaatc aaaccactgt ttccgcttat agagatcccc
      601 gcattctggg atgatgatga gggcattgaa cttataaaac acctttcctg gtatatctct
      661 gcaaccgtcg tgctcgcaga ctggacgggt tcgtcaacgc gattttttcc acgcgtcgca
      721 cacccaatgg atattaaagg ttactggcag aaaactttaa ttcaggctca aaacgcctta
      781 accgtctttc ctctaaaagc aaaagtcgcg cctttcaacg gaattaatac gctgtttcct
      841 tttattgaga atccgacacc gttacagcaa aaggtgctgg atctggatat cagccagcaa
      901 ggaccgcagt tatttattct ggaagatgtg actggcgcag gtaaaacaga agcggcgctt
      961 atcctggcgc acaggttgat agctgcgggg aaagctcagg ggctgttttt tggtctgcca
     1021 acaatggcaa cggccaatgc catgtacgat cggctggtca aaacctggct tgctttctat
     1081 tcgccagaat cccgccccag cctggtgctg gcacatagtg cccgcacatt aatggaccgc
     1141 tttaatgaat cactctggtc tggtgattta gtcgggtcag aagaaccgga tgaacaaacg
     1201 ttcagtcagg gatgtgcggc ctggtttgcc gacagtaaca agaaggcgtt gctggctgaa
     1261 attggcgtcg gcacgctgga tcaggcgatg atggcggtga tgccgttcaa acataataat
     1321 ctgcggcttc tggggctgag taacaaaatc ctgctggctg atgagatcca cgcctgtgac
     1381 gcttacatgt cgtgcattct tgaagggttg atcgagcggc aggcgcgtgg cggaaacagc
     1441 gttattttgc tttctgccac gttatctcaa cagcagcgcg acaaactcgt cgctgccttt
     1501 gcgcggggta cagagggcct gcaagaagct ccgttcctgg aaaaggatga ttacccctgg
     1561 ctgacgcatg tcacgaaatc cgatgtgaac tcacaccggg tagcgacgcg caaagacgtt
     1621 gagcgtagcg tcagcgtggg ttggcttcat agtgaacaag agtgtattgc gcgtatcgaa
     1681 tcggcggtaa gtcaggggaa atgcatcgcc tggatccgga attctgtcga tgacgctatt
     1741 aaggttcatc gtcagctgct tgcccgcggc gtcattcctg cttccagcct ttcacttttt
     1801 catagccgct ttgcttttag cgatcgccag cgaattgaaa cggagacact ggcacgcttt
     1861 ggtaaatatt gtagtttaca gagagcgtcg caagtcattg tttgtacaca agtaattgag
     1921 caaagcgtgg atattgacct ggacgaaatg atctccgacc tggcccctgt tgatttgctg
     1981 attcagcgag caggacgatt acagcggcat atccgcgata ttaatggtca gttaaagcgt
     2041 gacggaaagg acgagcgttc cccccctgaa ttgctgattc tggctcccgt ctgggacgac
     2101 tctcctggcg acgaatggtt tggcagtgcc atgcgtaaca gtgcatttgt ctatcccgat
     2161 catgggcgaa tctggctgac gcagcgtgtg ctgcgtgagc aaggtgctat tcagatgcca
     2221 cacgcagccc ggcttcttat tgaatccgtc tacggtgagg acgtggtaat gccggaagga
     2281 tttgcccgca gcgagcagga gcaagtgggc aaatattact gtgatcgcgc aatggctaaa
     2341 aagtttgtgc tgaacttcag gcccggctat gccgccaata tcaacgatta cctcccggaa
     2401 aaactgtcga cacgtctggc tgaggagtct gtttccctgt ggctggctac ctgtattgac
     2461 ggtgtggtga agccttatgc caccggtgct cacgcatggg aaatgagcgt tgtcagagtg
     2521 cgccgaagct ggtggaaaaa acatcgggat gagttttctt tactggaggg agaggcgttc
     2581 aggctgtggt gcattgaaca gcgacaagat ccggagatgg caaacgtgat tttagtcaac
     2641 gatgacgaaa gttgcgggta ttcggctacg gaaggattga ttggcaaggt tggttgagat
     2701 gtctgcgaca ggactctctg aatagttgat acaccgaaaa ggatggaaag atggacaacc
     2761 gacctgggat ggtctttttt acgagaggat gcgtatgaac tcgttttcac ttctgacaac
     2821 cccgtggttg cctgttcgtt ttaaagacgg aacgacaggc aagctggcac cagtcgatct
     2881 ggctgatgaa aatgttgtcg atatcgctgc gccgcgggca gatctccagg gtgcggcctg
     2941 gcagtttttg ctggggctac tacaaaccag cttcgcgccc aaaaatcacg gtcgttggga
     3001 tgatatctgg caagacggac tggaggctga aaagctacgg gaagcattgc tgtccttaga
     3061 acacgctttc cagtttgggc cagattcccc ttcatttatg caggattttg atgaattaaa
     3121 agttaaggca acttctattg cttcgcttct tcctgacgca ccaggaaaac aaacaaaaga
     3181 acgaaataca gaccatttta ttaagcgaga cacaactcag catctgtgcc tgcattgtgt
     3241 gcctttagcg cttttttcaa tccagttgaa tgcacctata ggtggacgcg gttactatcc
     3301 tgggctacgt ggcggagggc ctctgacgac acttatagaa ttgctggagt atcagggcaa
     3361 tcaacaaacc cctttgtggc gaaagctgtg gctaaacgta atgccgcaag atgaagccga
     3421 tttaccttta ccaaaaacct ttgatgacct ggtctttcct tggcttgcac cgacgcgtac
     3481 cagcgaactg gacggtgctg tggtaacgga tgaacaagtc aataaacttc aggcctactg
     3541 gggaatgcca cgacgtatac gtattgattt taagacaacc tcaataggta actgcgacat
     3601 ttgcggtagg cagagcgatg ctcttttggg cttgatgtca ctcaaaaact atggtgttca
     3661 atacgtcatg tggcgccatc ctctaacacc ttaccgtctg ccgcttaaag agggtggaga
     3721 tttttactca gttaaaccac aacctggtgg tttaatctgg cgcgactggt taggcctcat
     3781 agaggtaggt aactcaaaaa acaatactga actccccgca caggtcgtga aacttttgaa
     3841 tgccagcaat ctaaagcaga caagggtggg cttatgggga tttggttttg attttgaaga
     3901 tatgaaggtt cgttgttggt atgaacacca tttcccgctg ttgttaaaca aaaaagaaga
     3961 cctaatacct aagttgcgtc tggcagcgca agcggcatca cacattctaa gcttgttaca
     4021 ccgagcgttg aaagaagcat ggttctctga gaagaaaacc acaaagcttg atttcggttt
     4081 tgtggatatt gacttctgga acaaaactca gcatcgattc ctgagattag tgcgcaaaat
     4141 tgaagaaggt caggatccgt acgaattact cagtaaatgg caaaaggaaa tgtggttatt
     4201 cgcgcgtcag gattttgacg atcgtgtctt taccaatcca tatgagcccg ttgatttgga
     4261 acgcgtcatg accgcacgca agaaatattt tacaacatca gcggagaagc aaaatgctaa
     4321 cgccgccagg gagaaaaagc aggaggctgc tgaatgagta ttgtaaaaga tgagcataaa
     4381 gttgcgctgc gtaaatggca tgaagagttg caggataaac gcggcgagcg tgccagtctg
     4441 cggcgtagta cgacggttaa tgatgtttgt ctgacggatg gatttcgcct gtttcttaag
     4501 aacagacaaa taaagtggca ggatgaaccc gagtggcgta tcaccgcact agcgctgata
     4561 gcagcagtta gcgccaatgt taaagctatt gacgaacggc agccttttgc cgcgcaactg
     4621 gcggcggtaa tgaaaaaggg gcggtttact cgtttgttgg cagtaaaaac gccggatgat
     4681 ttattgcggc agttacgccg tgcggtcaaa ttattaaacg gttccgtcaa tcttgactct
     4741 ctggcagagg gggtcttccg ctggtgccag gagagcgatg acctgctcaa tcatcaccgc
     4801 cgccagcagc gaccaacaga atttatccgt atccgctggg cgctggaata ttaccaggct
     4861 ggcgacgccg ataacgaaca aaattaataa aggactatct catgacaaca tttattcagc
     4921 ttcatttgtt aaccgcttac cctgctgcca atcttaaccg tgatgattcc ggctctccga
     4981 aaactgtgat tctgggtgga gcaacgcgtc tgcgcgtttc ctcgcaaagt ctgaagcgtg
     5041 cttggcgcac ttctgaactt tttgaacagg cgctggcggg ccatattggt gttcgtagcg
     5101 ggcgtattgc gcgtgaggcg gcaactatcc tgattgagaa aggaatcgaa gataaaaaag
     5161 ccatcgaatg ggcagtagag atagccgatt atcttgggaa agccaaaaaa gacaaaaaac
     5221 aaaaaaacga caaaaaacca aaagatccgc tcactagcgc cgaaactgaa cagctcgtcc
     5281 atatcagccc ggcagaattt gacgccgtaa aagcgctggc ccatcaactg gcagaagaaa
     5341 agcgcgcgcc aaaagagaaa gatctcgctt tgttacgtaa agaccgtatg gcggtagata
     5401 tcgctatgtt tggtcgtatg ctggcgaaaa aacccgagtt taatgtagaa gccgcctgcc
     5461 aggtcgcgca tgcgtttggt gtcagtgaaa cgattgtcga aaatgatttt ttcaccgccg
     5521 tagatgattt acgccaggct tctgaagatg ccggtgccgg tcatgtagat gaaaccggat
     5581 tcggttctgc gctgttctac acctatatct gcattgataa agatctgctg gtagaaaacc
     5641 tcggcggtga cgaagcgtta gctaatcaga ccatacgcgc ctttacggaa gccgcgctta
     5701 aagtctcccc aaccggtaaa caaaacagct ttgccagccg ggcttatgcc tcctgggcgc
     5761 tggcagaaaa aggcaccgat caaccgcgtt ctctggcggc ggctttctat gaacccatta
     5821 atggcacacg tcagttagat gtggcggtgc agcgtattac aacgcttcgc gaaaacatga
     5881 atacggtcta cgaacagaag accgaatgcg caagttttga cgtgatgaac aaacagggaa
     5941 gcatgaagga cgtgctggac tttatctgcg cgtaaggggc ggcttatgag ccaatatctg
     6001 atttttcagc ttcatgggcc gatggcgtcc tgggggggcg atgctccagg tgaagttcgt
     6061 catacccatg aacttccttc gcgctcagca ttgctggggc tactggctgc cggggtaggg
     6121 attcggcgtg atgataccga acgcttaaac gcgtttaatc gccactattc actggtggtt
     6181 tgcgccagcc gtaacccgcg ctgggcacgg gattatcaca cggtccagat gccaaaagag
     6241 gtgcgtaaag cacgttattt cagccgtcgc gaagagttga gcgaccctga gcttttgagc
     6301 gcgattatct cccggcgcga ctactacacc gatgcctggt ggatggtggc ggtggcaaca
     6361 acagccgatg cgccttacag cctcgaacag ttgcaggacg gtttacgcca tccagttttt
     6421 ccgctttatc tggggcgaaa aagtcatccg ctggcattac ctcttgcgcc gttactgctc
     6481 gaaggcaacg cgtgtgatgc cttatgtaat gcataccaac agtatcagga tcatttccat
     6541 aaactgaaag tctcactccc gaaacttcag gatgaatgct ggtgggaagg gaaacacgac
     6601 ggcctggtcg cgagcaaaat attacgccgc cgggatgttc ctttaaatcg tcagcagtgg
     6661 ctgtttgggg aacgcaccgt caatcagggg ccgtggctca gcaaggagga accatgtacc
     6721 tctcaagaat aaccttacat accggccagc tttcgcctgc gcagttgctg catctggtgg
     6781 atcgcggtga atatgtgatg catcagtggc tgtgggatct cttccctggc ggcaaagaaa
     6841 ggcaatttct ttatcgtcgg gaagaactcc agggcgcgtt tcgctttttt gtactgtcgc
     6901 aggaacgccc ggcggaaagt gatacgttca ccatagaatg ccggtcattt gcgcccgagc
     6961 tgtgtaccgg acaacaactt tgtttcaacc tgcgggcgaa tccaacaatc tgtaaagcgg
     7021 gcaagcgcca cgatctgctg atggaggcga aacggcaggt gagggggcag gcggaaggaa
     7081 gtgatgtctg gttgcatcaa caacaggcgg cgctggactg gctggcagcg cagggggaga
     7141 gaagcggttt tacactgctg gatacctcgg ttgatgccta ccgacaacag caactgcggc
     7201 gggaaaactc ccggcaactg atccagttca gtagcgtgga ttatacgggg atgcttacgg
     7261 tcaccgatcc tgggttattt gtgcaacgcc tgagccaggg gtatggtaaa agccgggcgt
     7321 tcggctgtgg tctgatgctg atcaaacccg gagcagaggc gtgacgtttg taccactgag
     7381 cccgatccca ttaaaagatc gcacctccat gatcttcctc cagtacggtc agatcgacgt
     7441 actggacggc gctttcgtgc tgatcgacaa aacagggatc cgaacgcata ttccggtggg
     7501 atcggtcgcc tgcattatgc tcgaaccagg aacgagagtt tcccacgcgg cggtgcatct
     7561 ggccgccacg gtgggaacac tgctggtctg ggtcggtgaa gcgggcgttc gcgtttactc
     7621 ctccggacaa cccggtgggg cgcgggcaga taaattactc taccaggcaa agctggcttt
     7681 aacggaagat ctacggttga aggtggtgcg caaaatgtat gaattacgtt ttcgtgaacc
     7741 acagccagct cgccgttcag tggagcagtt acgtggaatt gagggatctc gcgttcgcca
     7801 gacgtatgca ttactggcga aacaatatgg tgtgaaatgg aatggtcgca aatacgatcc
     7861 taaagactgg gaaaaaggcg atgttgtgaa tcgctgcatc agtgctgcta catcatgtct
     7921 gtacggtata tctgaagcgg cagtattagc cgcgggatat gcgcccgcta ttgggtttat
     7981 ccatagtggc aaaccgcttt catttgttta tgacatagcc gatatcatta aatttgattc
     8041 ggttgtgcca aaggcatttg aaatagcagc gaggcaaccc gcagaacctg ataaagaagt
     8101 cagattagcc tgtcgcgata ttttccgtag cactaagtta acgggcaaat taataccgtt
     8161 aattgaggaa gtccttgccg caggtgaaat tgaaccgcca caacctgcgc cggatatgtt
     8221 accgccagcc atccccgaac ctgaaacgtt gggcgatagc ggtcatcgag gacgcggctg
     8281 atgagcatgg tcgtggttgt tacagaaaat gtcccgccgc gcttacgtgg acggctcgca
     8341 atctggctac tggaagtgcg tgccggtgtg tatgttggtg atacatcaaa acgtattcgg
     8401 gagatgatct ggcagcaaat tacccaactg gctggttgcg gaaatgtggt gatggcctgg
     8461 gcgaccaata ccgagtcggg ttttgaattt cagacctggg gagaaaacag acgtattccg
     8521 gtggatttgg atgggttacg tttggtttct tttcttcctg ttgataatca ataagttatc
     8581 cgttctttaa aaataaggaa atgttttaat ttagttggta gattgttgat gcggaataaa
     8641 tttgtttaaa aacagttatg tatgcttagt gtgttccccg cgccagcggg gataaaccga
     8701 cgaccaaatg atctacgcgt attgccacct cgtgttcccc gcgccagcgg ggataaaccg
     8761 ttaacgtctg gatcctgtgt gccatggacc gtgtgttccc cgcgccagcg gggataaacc
     8821 gactgataac gccggagcct tctttttcag caagtgttcc ccgcgccagc ggggataaac
     8881 cgaggttgac gttgattttg ttcgttatgt tgccgtgttc cccgcgccag cggggataaa
     8941 ccggcaattt gttgtccgcg atccggtacg cgcgtgtgtt ccccgcgcca gcggggataa
     9001 accgcggcta tggaatttat ggagaagttt ggttttgtgt tccccgcgcc agcggggata
     9061 aaccgggcgc actggatgcg atgatggata tcacttagaa ttccccgccc ctgcggtaga
     9121 actcccagct cccattttca aacccatcaa gacgccttcg ccagctcctt caccaacggt
     9181 agcattatcc gcataacgtc acggcagcga cgttctattc ttccaggaag tgccttatca
     9241 atatgctgtt gattatccag tcttacgtca tgccagctat ttcccgcagg gaatgcggct
     9301 gtttttgcgc gttgctgata accatcctta ttcccaagat tccagttagt cgcttccacc
     9361 gaaagtacag caatgcccgc tttgtcgaat atttctgcgt cattacaaca cccagtgcct
     9421 ttcggataat ttttattcaa acccggattg gtcgttgcgg ctattccgtg actgcgcgca
     9481 attgccagcg ccctgtcgcg cgttaatttc cttactgctt caggggtttt tacaccgctg
     9541 ttgaaataca atttatcgcc aacaattaag ttatcgagat taatcaccag cagcgtattt
     9601 ttcttttcgg tgtcactcat ccgcttgagt aaattctcag cgcctaattt cccttcctct
     9661 tcgccgctgg tcgccacaaa tcgaatacca tactcggtag gcgtattttt caggcgttct
     9721 gccaattcca gcatgacacc taaacctgcg gcgttatcat ccattccttg taacgtcagc
     9781 ccgccgagat tggcatcggc gtcagcatcg ctcagcgggg cgtaagtatc cagatgcgcc
     9841 ataatgatga tctgctgcgg cgctttgcct tcatgagcgg caatcaccgt acttcctgtc
     9901 acgttatgcc agttcttgcg attatcgcgg gcggtataaa tataccgact attaaatgtc
     9961 cgaatatcac tgcgataacc catttgctga aactgttggc gaatataatc ggcagataac
    10021 atttctgctg gagttccggt catgcgtccc ggaaagaaag tagcaatatg tcgtgcctgg
    10081 gtattagcaa aatcgccagg tttaggtgac gaggcgtgta cggggagaat aaagcatacg
    10141 ccgagcgcca gggcagcggt acggtggcgc aatgcggaaa acatagtgag tccttaaata
    10201 ccatgcaaat ttttttaccg ccatagtatg aaactgccgc tgcgctaaaa caatttcaaa
    10261 tcttcctaaa tgcccgaaat ccggtgcctt aagcactttt tgatattagc tttgccaaat
    10321 cgttattccg ttaaggaact actcattcta attggtaatt tcattcgttc tcttacgctc
    10381 cctatagtcg aaacatctga tggcaagaaa atagcggtat tgcaaaggaa cggttatgga
    10441 tcaaaaacga cttactcacc tgcggcaact ggaggcggaa agcatccaca ttattcgcga
    10501 ggtggcggca gaattctcaa atccggtgat gctctactct atcggtaaag attccagcgt
    10561 catgctgcat ttggcgcgca aggcgtttta tccaggtacg ctgcctttcc ccttgctgca
    10621 tgtcgatacc ggctggaaat tccgcgagat gtatgagttc cgcgatcgta cggcgaaagc
    10681 ctacggctgc gagctgctgg tgcataaaaa cccggaaggc gtggcgatgg ggattaatcc
    10741 attcgtgcac ggcagcgcga aacataccga tattatgaag accgaaggcc tgaagcaggc
    10801 gctgaataaa tacggttttg atgccgcctt cggtggtgcg cgccgcgacg aagagaaatc
    10861 ccgtgctaaa gagcgtatct actctttccg tgatcgcttc catcgctggg acccgaaaaa
    10921 tcagcgcccg gagctgtggc acaactataa cgggcagatt aacaaaggcg aaagcatccg
    10981 cgttttcccg ctctctaact ggaccgagca ggatatctgg caatacatct ggctggaaaa
    11041 tatcgacatt gtgccgctgt atctcgctgc ggaacgtccg gttctggaac gcgacggtat
    11101 gttgatgatg attgatgaca accgtatcga cctgcaaccg ggcgaagtga ttaaaaaacg
    11161 gatggtgcgt ttccgtacgc tgggctgctg gccgctgacc ggtgcggtgg agtcaaatgc
    11221 acaaacactg ccggagatca tcgaagagat gctggtttcc accaccagtg aacgtcaggg
    11281 ccgcgtgatt gaccgcgacc aggcggggtc tatggagctg aaaaaacgtc aggggtattt
    11341 ttaagatgaa caccgcactt gcacaacaaa tcgccaatga aggcggcgtc gaagcctgga
    11401 tgattgcgca acaacataaa agcctgctgc gttttctgac ctgtggtagc gtcgatgacg
    11461 gcaaaagtac cctgattggt cgcctgctgc acgatacccg ccaaatctac gaagatcagc
    11521 tctcatcgct gcataacgac agtaagcgtc acggcaccca gggcgaaaaa ctggatctgg
    11581 ctctgctggt ggacggcctg caagctgagc gcgaacaggg catcactatt gatgtggcct
    11641 accgctattt ctctaccgag aagcgtaaat ttattatcgc cgacaccccc gggcacgagc
    11701 agtatacccg caatatggcg accggcgcct caacgtgtga gctggcgatc ttactgatcg
    11761 atgcccgtaa aggcgtgctc gatcaaaccc gtcgtcacag ttttatctcc acgctgctgg
    11821 ggatcaaaca tctggtcgta gcgatcaaca aaatggatct ggtggattac agtgaagaga
    11881 cattcacccg cattcgtaaa gactacctga ctttcgccga acagttacct ggcaatctgg
    11941 atattcgctt tgtgccgctc tctgcgctgg aaggcgacaa cgtggcttcg caaagtgaaa
    12001 gtatgccgtg gtacagcggt ccgacactgc tcgaagtgct ggaaaccgtg gagatccagc
    12061 gagtggtgga tgctcagcca atgcgcttcc cggtgcagta cgttaatcgc ccgaatctcg
    12121 attttcgtgg ttacgccgga acgctggcat ccggtcgcgt ggaagtcggg caacgtgtaa
    12181 aagtgctgcc ttctggtgtg gaatcaaacg tcgcgcggat cgtgactttt gatggtgatc
    12241 gcgaagaagc ctttgccgga gaagcgatca ccctggtgct gacggatgag atcgacatca
    12301 gccgtggcga tctgctgctg gcggcagacg aagcgttacc ggcggtgcag agcgcgtcgg
    12361 tggatgtggt atggatggcg gaacagccgc tttctccagg gcagagttac gacatcaaaa
    12421 ttgccggtaa gaagacgcgc gcgcgtgttg atggcattcg ctatcaggtt gatattaata
    12481 accttaccca gcgtgaagtt gaaaacctgc cactgaatgg gatcggcctc gtggatctca
    12541 cttttgacga gccgctggtg ttagatcgtt atcaacaaaa tccggtgacg ggtgggctga
    12601 tttttatcga tcgcctgagc aatgtgaccg tgggtgccgg tatggtgcac gagccagtta
    12661 gccaggcaac tgctgcgcca tctgaattca gtgcattcga actggaattg aatgctctgg
    12721 ttcgtcgcca ctttccgcac tggggcgcgc gcgatttgct gggggataaa taatggcgct
    12781 gcatgacgaa aacgtcgtct ggcatagcca tccggtcact gtgcaacaac gcgagctaca
    12841 ccacggtcat cgtggtgtag tgctgtggtt taccggcctc tccgggtccg gtaaatcaac
    12901 ggtcgccggg gcgctggagg aggcgttaca taaactcggc gtcagtacgt atctgctgga
    12961 tggcgataat gttcgccacg gattatgcag cgatctcggt tttagcgatg ccgatcgtaa
    13021 agagaatatc cgtcgcgtcg gtgaagtggc gaatttgatg gttgaagccg gactggtagt
    13081 gctgaccgca tttatctcgc cacaccgcgc cgaacgccag atggttcgcg aacgcgtagg
    13141 agaagggcgc tttatcgaag tgtttgtcga tacgccgctg gcgatttgcg aagcccgcga
    13201 tccaaaaggc ttatataaga aagcgcgtgc cggtgaactg cgcaacttta cgggaataga
    13261 ttccgtttac gaagcgcctg aatcggcaga aattcatctc aatggtgaac aattagtaac
    13321 aaatttggta cagcaattat tagatctgtt gagacagaac gatattatca gatcctgaga
    13381 caccaccggg tttccatgcc cggttcgtca aagtaacagg atcaaatatg cgtaatagcc
    13441 ataacattac actaacaaat aacgacagcc ttactgagga tgaagaaacc acatggtcac
    13501 tgcctggtgc cgtggtcggt tttatctcct ggttatttgc gctggcgatg ccgatgttga
    13561 tttatggctc taacacgctg ttcttcttta tctacacctg gcctttcttt ctggcgctga
    13621 tgcccgtcgc ggtagtggtg gggattgcgc tgcattcatt gatggacgga aagctacgct
    13681 acagtattgt tttcaccctg gtgacagtag gcattatgtt tggcgcactg tttatgtggc
    13741 tactgggcta atttgtactt tcccgtctcc tctgttcata attcaaaccg taactaataa
    13801 tgagattatg ttctgcacgc cctgggtata cgtaacaatg gacaaatgtg gtacatttgc
    13861 ccgcgttgtc gcggtatccc caacagagga tgtagagtcg tcttcggatg catgggatga
    13921 tgatgccgtt tttcaggggg caggatgggt aaactaacgc tgctgttgct ggctattctg
    13981 gtctggctac agtattcgct gtggttcggt aagaacggta tacatgacta tacccgcgtc
    14041 aatgatgatg tggcggcaca gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcaa
    14101 ctttttgccg aaattgacga tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt
    14161 aatgaactca gcatgaccag gccgggcgaa actttttatc gtctggtgcc tgacgcgtcg
    14221 aagcgcgcac agtctgcggg gcaaaacaat cgataaatca gcccgggaat taacatggca
    14281 accactcatt tggatgtttg cgccgtggtt ccggcggccg gatttggccg tcgaatgcaa
    14341 acggaatgtc ctaagcaata tctctcaatc ggtaatcaaa ccattcttga acactcggtg
    14401 catgcgctgc tggcgcatcc ccgggtgaaa cgtgtcgtca ttgccataag tcctggcgat
    14461 agccgttttg cacaacttcc tctggcgaat catccgcaaa tcaccgttgt agatggcggt
    14521 gatgagcgtg ccgattccgt gctggcaggt ttgaaagccg ctggcgacgc gcagtgggta
    14581 ttggtgcatg acgccgctcg tccttgtctg catcaggatg acctcgcgcg attgttggcg
    14641 ttgagcgaaa ccagccgcac gggagggatc ctagccgcac cagtgcgcga tacgatgaaa
    14701 cgtgccgaac cgggcaaaaa tgccattgct cataccgttg atcgcaacgg cttatggcac
    14761 gcgctgacgc cgcaattttt ccctcgtgag ctgttacatg actgtctgac gcgcgctcta
    14821 aatgaaggcg cgactattac cgacgaagcc tcggcgctgg aatattgcgg attccatcct
    14881 cagttggtcg aaggccgtgc ggataacatt aaagtcacgc gcccggaaga tttggcactg
    14941 gccgagtttt acctcacccg aaccatccat caggagaata cataatgcga attggacacg
    15001 gttttgacgt acatgccttt ggcggtgaag gcccaattat cattggtggc gtacgcattc
    15061 cttacgaaaa aggattgctg gcgcattctg atggcgacgt ggcgctccat gcgttgaccg
    15121 atgcattgct tggcgcggcg gcgctggggg atatcggcaa gctgttcccg gataccgatc
    15181 cggcatttaa aggtgccgac agccgcgagc tgctacgcga agcctggcgt cgtattcagg
    15241 cgaagggtta taccctgggc aacgtcgatg tcactatcat cgctcaggca ccgaagatgt
    15301 tgccgcacat tccacaaatg cgcgtgttta ttgccgaaga tctcggctgc catatggatg
    15361 atgttaacgt gaaagccact actacggaaa aacttggatt taccggacgt ggggaaggga
    15421 ttgcctgtga agcggtggcg ctactcatta aggcaacaaa atgattgagt ttgataatct
    15481 cacttacctc cacggtaaac cgcaaggcac cgggctgctg aaagccaatc cggaagactt
    15541 tgtggtggtg gaagatttgg gctttgagcc tgatggtgaa ggtgagcata ttctggttag
    15601 aatcctcaaa aacggctgca atacccgttt tgtggcggat gcactggcga aattcctgaa
    15661 aattcatgcc cgtgaagtca gcttcgctgg gcaaaaagac aaacatgctg ttacggaaca
    15721 gtggttatgc gctcgcgtgc caggcaagga aatgcccgat ctgagcgcct ttcaactgga
    15781 aggctgccag gtgctggagt atgcgcggca caagcgcaag ctgcgtttag gcgcgctgaa
    15841 aggtaacgcc tttaccctgg ttctgcgcga agtgagcaat cgcgatgacg ttgaacaacg
    15901 tctgatcgat atttgcgtaa aaggtgtacc gaactacttc ggtgcccaac gttttgggat
    15961 tggcggtagc aacttgcagg gggcgcagcg ctgggcgcaa accaatactc cggtgcgcga
    16021 tcgcaataaa cggagttttt ggttgtcggc agcccgcagt gcgttgttta atcagattgt
    16081 tgctgagcgc ctcaaaaaag cagacgttaa tcaagttgtt gacggcgatg cgctacaatt
    16141 agccggacgt ggtagctggt ttgtcgcaac caccgaagaa ctggcggaat tacagcgtcg
    16201 cgtcaacgat aaagagctga tgataaccgc cgcattgccg ggcagtggcg aatggggaac
    16261 tcagcgtgaa gcgctggcat tcgaacaagc agctgtcgcc gcagaaactg aattacaagc
    16321 tttactggtg cgcgaaaaag ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca
    16381 attaagctgg aattggtggg atgacgtcac cgtagagatc cgtttctggc ttccggcggg
    16441 tagttttgca accagcgttg tcagggaact tatcaacaca acaggtgatt atgcgcatat
    16501 tgctgagtaa tgatgacggg gtacatgcac ccggtataca aacgctggcg aaagccttgc
    16561 gtgagtttgc tgacgttcag gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt
    16621 ctctgacact ggaatcctcc ctgcgcacgt ttacctttga aaatggtgat attgctgtgc
    16681 aaatgggaac cccgaccgat tgcgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc
    16741 cggacattgt tgtgtccgga attaacgccg ggccgaatct gggggatgat gttatttatt
    16801 ccggtacggt agccgccgcg atggaaggcc gtcatttagg ttttccggcg cttgccgtct
    16861 cgcttgacgg gcataaacat tacgacactg ccgcggcggt aacctgttca attttgcgcg
    16921 cactgtgtaa agagccgctg cgcaccgggc gtattcttaa tattaacgtt ccggatttac
    16981 ccttggatca aatcaaaggt attcgcgtga cgcgctgcgg tacacgacat ccggcagatc
    17041 aggtgatccc gcagcaagat ccgcgcggca atacgctgta ctggattggc ccgccgggcg
    17101 gtaaatgtga tgccggtccg gggaccgatt ttgctgcggt agatgagggc tatgtctcca
    17161 tcacgccgct gcatgtggat ttaactgcgc atagcgcgca agatgtggtt tcagactggt
    17221 taaacagcgt gggagttggc acgcaatggt aagcagacgc gtacaagcac ttctggatca
    17281 attacgtgcg caaggtattc aggatgagca ggtgctgaat gcacttgccg ccgtgccgcg
    17341 tgaaaaattc gttgatgaag cgtttgaaca aaaagcctgg gacaatatcg ccttgccgat
    17401 aggtcagggg cagacaattt cgcagccgta tatggtggcg cgaatgaccg aattactcga
    17461 gctgacgccg cagtcgcggg tgctggaaat tggcactggt tcgggatatc aaacggcaat
    17521 cctggcgcat cttgtccagc atgtttgctc ggtcgaacgg attaaaggtt tgcagtggca
    17581 ggcgcgtcgc cgcctgaaaa atcttgattt acataatgtt tcaacccgtc atggcgatgg
    17641 atggcaaggt tggcaggcac gcgcgccgtt tgacgctatc attgttacgg cggcaccgcc
    17701 ggaaattcca actgcgctaa tgacgcagct ggacgaaggc gggattctcg tcttacccgt
    17761 aggggaggag caccagtatt tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga
    17821 taccgtggag gccgtgcgct ttgtcccttt agtgaagggt gagctggctt aaaacgtgag
    17881 gaaatacctg gatttttcct ggttattttg ccgcaggtca gcgtatcgtg aacatctttt
    17941 ccagtgttca gtagggtgcc ttgcacggta attatgtcac tggttattaa ccaatttttc
    18001 ctgggggata aatgagcgcg ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt
    18061 cactggtttc gctatggctg gcaggctgtt ctgacacttc aaatccaccg gcaccggtca
    18121 gctccgttaa tggcaatgcg cctgcaaata ccaattctgg tatgttgatt acgccgccgc
    18181 cgaaaatggg gacgacgtct acagcccagc aaccgcaaat tcagccggta cagcagccac
    18241 aaattcaggc tactcaacaa ccgcaaatcc agccagtgca gccagtagct cagcagccgg
    18301 tacagatgga aaacggacgc atcgtctata accgtcagta tgggaacatt ccgaaaggca
    18361 gttatagcgg cagtacctat accgtgaaaa aaggcgacac acttttctat atcgcctgga
    18421 ttactggcaa cgatttccgt gaccttgctc agcgcaacaa tattcaggca ccatacgcgc
    18481 tgaacgttgg tcagaccttg caggtgggta atgcttccgg tacgccaatc actggcggaa
    18541 atgccattac ccaggccgac gcagcagagc aaggagttgt gatcaagcct gcacaaaatt
    18601 ccaccgttgc tgttgcgtcg caaccgacaa ttacgtattc tgaatcttcg ggtgaacaga
    18661 gtgctaacaa aatgttgccg aacaacaagc cagctgcgac cacggtcaca gcgcctgtaa
    18721 cggtaccaac agcaagcaca accgagccaa ctgtcagcag tacatcaacc agtacgccta
    18781 tctccacctg gcgctggccg actgagggca aagtgatcga aacctttggc gcttctgagg
    18841 ggggcaacaa ggggattgat atcgcaggca gcaaaggaca ggcaattatc gcgaccgcag
    18901 atggccgcgt tgtttatgct ggtaacgcgc tgcgcggcta cggtaatctg attatcatca
    18961 aacataatga tgattacctg agtgcctacg cccataacga cacaatgctg gtccgggaac
    19021 aacaagaagt gaaggcgggg caaaaaatag caaccatggg tagcaccgga accagttcaa
    19081 cacgcttgca ttttgaaatt cgttacaagg ggaaatccgt aaacccgctg cgttatttgc
    19141 cgcagcgata aatcggcgga accaggcttt tgcttgaatg ttccgtcaag ggatcacggg
    19201 taggagccac cttatgagtc agaatacgct gaaagttcat gatttaaatg aagatgcgga
    19261 atttgatgag aacggagttg aggtttttga cgaaaaggcc ttagtagaag aggaacccag
    19321 tgataacgat ttggccgaag aggaactgtt atcgcaggga gccacacagc gtgtgttgga
    19381 cgcgactcag ctttaccttg gtgagattgg ttattcacca ctgttaacgg ccgaagaaga
    19441 agtttatttt gcgcgtcgcg cactgcgtgg agatgtcgcc tctcgccgcc ggatgatcga
    19501 gagtaacttg cgtctggtgg taaaaattgc ccgccgttat ggcaatcgtg gtctggcgtt
    19561 gctggacctt atcgaagagg gcaacctggg gctgatccgc gcggtagaga agtttgaccc
    19621 ggaacgtggt ttccgcttct caacatacgc aacctggtgg attcgccaga cgattgaacg
    19681 ggcgattatg aaccaaaccc gtactattcg tttgccgatt cacatcgtaa aggagctgaa
    19741 cgtttacctg cgaaccgcac gtgagttgtc ccataagctg gaccatgaac caagtgcgga
    19801 agagatcgca gagcaactgg ataagccagt tgatgacgtc agccgtatgc ttcgtcttaa
    19861 cgagcgcatt acctcggtag acaccccgct gggtggtgat tccgaaaaag cgttgctgga
    19921 catcctggcc gatgaaaaag agaacggtcc ggaagatacc acgcaagatg acgatatgaa
    19981 gcagagcatc gtcaaatggc tgttcgagct gaacgccaaa cagcgtgaag tgctggcacg
    20041 tcgattcggt ttgctggggt acgaagcggc aacactggaa gatgtaggtc gtgaaattgg
    20101 cctcacccgt gaacgtgttc gccagattca ggttgaaggc ctgcgccgtt tgcgcgaaat
    20161 cctgcaaacg caggggctga atatcgaagc gctgttccgc gagtaagtaa gcatctgtca
    20221 gaaaggccag tctcaagcga ggctggcctt ttttctttcg gttggtgagt cggtgtattt
    20281 gtagtctgga taagacgcga tagcgtcgca tccggaaatt tgccccctat gcgttcatca
    20341 tcttgcgcac cagctgcata aacagctcct gctcttctcc actcaaacgc cccaaaaatt
    20401 cctcatccac gctgtcacca atgggtatcg ccgcagcaag tatcttttcg ccttcggcag
    20461 tcaaccagac aaagcgtcgc cgcttatcag cggggtcatg ttcacgtctg actaagccac
    20521 gattttccat tcttgccaac atttctgcta acgttgcttt ggtgctgaca gctgcttcta
    20581 tcagtgccac ctgttcgata ccaggtttat cagcaatggc gcgcatcacc gcgtactgtg
    20641 gtttggtcag gtcaggtaac tcgtgctgcc agcgagccgt gtgctgctga aaaagctgtc
    20701 gtaactgatg gaacgcttta tttcgtaacg ccatgtaaac tccgggctaa ttctctgtcg
    20761 ctatcatagc ggttttttag tgacgaagaa gatgttgtta cttttcaatc cttgccgctg
    20821 tggaaatgaa ggagtatgtt aataataacg ttcgtatacg aacaattaag aggataagca
    20881 atgaaactga tcgtcgggat gacaggggct accggtgcgc ctcttggtgt ggcattactg
    20941 caagcgctgc gggagatgcc gaatgtcgag actcatctgg tgatgtcgaa gtgggcgaaa
    21001 accaccattg aactggaaac gccttacagc gctcgcgatg ttgctgccct cgcagacttc
    21061 agccataacc cggcggatca ggcggcgatc atctcatccg gttcttttcg taccgacggc
    21121 atgatcgtta ttccgtgcag tatgaaaacg ctcgccggta tccgcgctgg ttacgccgat
    21181 ggcctggtag ggcgcgcggc ggacgtcgtg ctcaaagaag gccgcaaact ggtgctggtg
    21241 ccgcgtgaaa tgccgcttag caccatccat ctcgaaaata tgctcgcact ttcacgcatg
    21301 ggcgtggcga tggtgccgcc gatgcctgcc ttttataacc atcccgaaac ggtagatgac
    21361 attgtccacc atgtggtagc ccgcgtgctg gatcaatttg gcctcgaaca tccccacgcc
    21421 aggcgctggc aaggattgcc gcaggcccgg aatttttctc aggagaatga ataatggcat
    21481 ttgatgattt acgcagcttt ttacaggcgc ttgatgacca cggccagtta ctgaaaatca
    21541 gcgaagaagt gaacgccgag ccggatctgg cagcagcagc taacgccacc gggcgtatcg
    21601 gcgacggcgc gcccgcgctg tggtttgata atattcgcgg ctttaccgat gcccgcgtgg
    21661 cgatgaacac catcggttcc tggcagaacc acgcgatttc cctcggcctg ccgccaaatg
    21721 ccccggttaa aaagcagatt gatgagttta tccgccgctg ggataacttc ccgattgccc
    21781 cggagcgccg cgccaatcca gcctgggcgc agaacaccgt tgatggcgac gagatcaacc
    21841 tgttcgatat cctgccgctg tttcgtttaa acgatggcga tggcggtttc tgtctcgaca
    21901 aagcgtgcgt ggtttcccgc gatccgctcg acccggataa cttcggcaag cagaacgtcg
    21961 gcatctaccg catggaagtg aagggcaagc gtaagctcgg cctgcaaccg gtgccgatgc
    22021 acgatatcgc cctgcatctg cataaagcag aagagcgcgg tgaagatctg ccgattgcga
    22081 tcacgctcgg taacgatccg atcatcacgc tgatgggggc cacgccgctg aaatatgatc
    22141 agtccgagta cgaaatggca ggcgcgctgc gtgaaagccc gtacccgatc gccaccgccc
    22201 cgttgaccgg ttttgatgtg ccgtggggtt cagaagtgat cctcgaaggg gtcatcgaaa
    22261 gccgtaaacg cgaaatcgaa gggccgttcg gtgagtttac cgggcactac tccggcgggc
    22321 gtaacatgac cgtggtgcgc atcgataaag tctcttaccg caccaggccg attttcgaat
    22381 cgctgtacct cggtatgccg tggaccgaaa tcgactacct gatggggcca gccacctgcg
    22441 tgccgctgta tcagcagctg aaagccgagt tccctgaagt gcaggcggta aacgccatgt
    22501 acacccatgg cctgctggcg attatctcca ccaaaaaacg ctacggcggc tttgcccgcg
    22561 cggtgggcct gcgcgcaatg accacgccgc atggtctggg ctacgtgaag atggtgatta
    22621 tggtcgatga agacgttgac ccgttcaacc tgccgcaggt gatgtgggcg ctctcctcga
    22681 aagtgaaccc ggcaggggat ttggtgcagt tgccgaatat gtccgtgctg gaactcgatc
    22741 caggctcaag ccctgcgggg atcaccgaca agctgattat cgacgccact acgcctgtcg
    22801 ccccggacaa ccgtggtcac tacagccaac cggtggtgga tttaccggaa accaaagcct
    22861 gggctgaaaa actgaccgct atgctggctg cacgtaaata aggagaagaa gatgatttgt
    22921 ccacgttgtg ccgatgaaca gattgaagtg atggcgaaat cgccggtgaa agatgtctgg
    22981 acggtatatc agtgccagca ttgcctttat acctggcgcg ataccgaacc gctgcgccgt
    23041 accagccgcg aacattatcc cgaagcgttc cgcatgacgc agaaagatat tgatgacgcg
    23101 ccaatggtgc cgagcatccc gccgctgctg gtggaaggta agcgttaaat aaaaggtccg
    23161 atgcccatcg gaccttttta ttaaggtcaa attaccgccc atacgcacca ggtaattaag
    23221 aatccggtaa aaccgagaat ggtcgttaac actgtccagg ttttcagacc gtctgctaca
    23281 gacaacccca gatatttggt cacaatccag aaccctgagt cattaatatg tgacgcgcca
    23341 agcccaccaa agcaggctgc cagcgtcacc aatacgcact gaatcggatt caatcccatc
    23401 accgcttctg agagtaaccc gccggttgtc aggattgcta cggttgctga cccctgcgat
    23461 gcacgcagag ccagtgaaat aataaatgcg gctggtaaca gaggcaggtc aatcatttgt
    23521 agcatattgg caagggcttt gccgacgccc gattccacca gcactttgcc aaatacccct
    23581 ccagcaccag taaccaaaat cactaccgcc gcagtgggaa gggctgagcc cataatgtcg
    23641 ctggtgtgtt gtaagctcca gccgcgacgt aaagccaata accagaatgc cagcaccagc
    23701 gcaatcatta gagctaccat tggtgagccg atcagctgta gcgtaccaag caggggatgc
    23761 gaaggcggca tcagtgttgc ggaaaccgta cccgccatga taatcgcgat aggaataaca
    23821 attagcgagg tgaccagcgc gacgcccggt ggatttattt tatcgcttaa ttttgttgcg
    23881 ccttcctcac tggccggagc cagttgcatc tgttccagta cttccaccga catcgcatat
    23941 tggcgcgtat tgattatttt cgctgcaaag tagccaacaa tccctacggg aatagaaatc
    24001 gcaataccga tgatggttag ccagccgatg tctgcgtgga gtaaccccgc tgcggcgaca
    24061 gggcctggat gcggcggtac cgccacatga acagttagca tgattccagc gacaggcagg
    24121 ccaaatttga gtggcgatat tttggcaacc ttggcaaaac cgtaaatgat tggcgcaaga
    24181 ataataaagc cgacatcaaa gaagacggga ataccgagga agaacgctgc cagagtcagc
    24241 gcagcgatag ttcgtttgtc acctaacttg cgactgaaat aattagccag tgactctgca
    24301 ccaccagagt gttcgatcat acgccccagc atagcgccca gaccaataat aatagtgacg
    24361 gaaccaagca caccgcccat cccggcgatc atcactttac ccacttcgcc cgccggtata
    24421 cccgccgcaa gtgcgactaa caggctgacg aggagcagag caacgaatgg ttgtaccttt
    24481 gccttgatga ccagcagcaa cagcatgatt acgccagcta acgcaatgca taacaatgta
    24541 attgtggaca tgggaaaccc tgtctgaaag ttatagttaa cctaccccat ccgtagatgg
    24601 ggggatgtat gggtacgttg taattaggga tttaacgaat tagcgccagg cgtcaaacca
    24661 gccaagccct tcttcggtga ggccacgagg tttatattca caaccgatcc agccctgata
    24721 tcccacctca tcgaacaggc ggaacagcca cggatagttg atttctccat cgtccggttc
    24781 atgtcgatca ggtagtccgg caatttgtac gtgcgcatat ttcccagcgt agtcgcggat
    24841 taaatgcgtc aggttgccat ctactttttg cgcatgaaaa gtatctagtt gaataaacac
    24901 gttatctcgc gcaacctctt caacaatagc cagtgcctga tactggctgg agaagagata
    24961 atgaggctta acgtcggggc tgagtgcttc aactaatatt cgcttgccgt gtagcgcaaa
    25021 gcggtcggca gcgtagcgga gattatcgat aaatactgcc cggtaccgtt cagcgtcttc
    25081 gccagcgggc acgacgcctg ccatcacatg gacttgttca caattgagcg ccaatgcata
    25141 ttccagtgcc aggtcgatgt ctgcgtgtgc ttcgtgctca cgtccgggaa gggcggataa
    25201 tccccattcc cccgcattaa tatctccggg agcggtattg aacagcgcca gtgtcagatg
    25261 gttttgctcc agttgctttt ggatttgcag ggtggagtag tcatagggaa acagaaattc
    25321 cacagcatcg aacccggctt ttcgcgctgc ggcgaagcgt tcaataaaag gcacttcggt
    25381 gaacatcatg gataaattag ctgcaaaacg aggcattgca ttaactcctt aattccgcaa
    25441 tttcacctgc ggtcagataa cggatcgggc ggtcaccgag aataaaaatc agctttgccg
    25501 tttcctccag ctcttccata ttgttggcgg cttcttgcag gctttcaccg caaaccactg
    25561 ggccatgatt tgccagtaaa aaagcctgat tgtctgctgc cagttccgcc agatcctgtg
    25621 cgatgcgttt atcgcccggt cggtaataag gcaccagcgg gacatttccc atccgcatca
    25681 ccacgtatgg tgtgaacgga cgaataacgt tgctgctgtc cagcccttgc aggcaggaaa
    25741 gcgccgtcga ccatgtgctg tgcaaatgca ccaccgcttt acagcgcgga ttgttgcgat
    25801 acagcgccag atgaaagagc acctctttcg agggtttgtc accacttaac cattcgccat
    25861 ccgcggcgac tttggaaagc cgctgcggat cgagattgcc caggcatgaa cctgtcggtg
    25921 tcgccagtaa attcccgtca ggtaaaagca gcgacagatt gccagccgaa ccggttgcat
    25981 agccgcgctg aaagaatgaa ctggcaatcc gcgtcatctc ctctcgcaaa gactgctcta
    26041 cttttgcgaa atcgctcatg ataaaaactc tctttgggct cgtgaaaaaa aggcttcatc
    26101 accgaagttg ccagatttaa gggcgagtga gacaggctta tccagtgcgt ttacccacgg
    26161 cacgccgggg gaaatggttg ggccaatatg aaaccctttt atgcccaggc tctgtgtgac
    26221 tacgccggag gtctcaccgc ctgcgacaat aaagcgtgtc acgccttccg ctgctaaccg
    26281 cgccgctagt tgagaaaata gagtttctac tgcctgactg gctttttgtg caccgtattg
    26341 ctgttgaatt gctgccaatg cgtcagtgct ggcggtggca aaaaccagtg gagcaagtac
    26401 actttcctgg cccagaaccc actctgccag ttcgtgtgca taagcggcca gagtttcggt
    26461 tgagaggcag cgtgccacat caacttcacg ggctggtgca atttgacggt aatgtgccac
    26521 ctggcggttg gtcatttgag agcatgaacc ggagagcact acgccgcgcc cagcgagcgg
    26581 atgccctgct tcgcgagcct ggttaccgtt ttcttgcgcc cactgccggg ccaggccaat
    26641 cgccagacca gaaccgcccg ttaccagtgg ggcatcgcgc aaggcttctc cctgaatttc
    26701 cagatggtgt tcggtcagcg catcaagcac cgcgtagcgg tagccctctt gctgtaagcg
    26761 agccagctct tgacgaacgg catccacacc ttgttcgaaa acatgtgccg aaacgacgcc
    26821 gcagcgccct gtggattgcg cttcaaccag acggggaaga tagctgtcgg tcatgggatt
    26881 taccgggtga tggcgcatcc cggattcggc cagcagttga ttcattacga acaaataccc
    26941 ctgataaacc gtacgtccgt tgaccggcag ggccggagag aagaccgtaa acggcgtgtc
    27001 gagagcatcc attaatgcat cggtaaccgg gccgatatta cctttcgccg tactgtcgaa
    27061 agtagagcag tatttgaaat agatctgttt gcaaccttgc tgttgcaacc agctcagagc
    27121 cgccagcgat tgctgtgtgg cttcaaccac cggacaggag cgcgttttca ggctgatcac
    27181 cagtgcgtcg attgcttccg gcattttacc tgttggaaca ccgttaattt gtaccgttgg
    27241 tagaccgttt tccaccagaa aactggcgat atccgtcgcg ccggtaaaat catcggcgat
    27301 aacgccaatc ttgatcatga tttcgctccc ggtagagtga tgccagagaa aatcttgata
    27361 actgcgctat cgtcttcttt cccgtaacct gcgttactgg cgctggtgaa catattcaat
    27421 gctgttgagg ccaatggcag cgggaagtgc agggctttgg ctgtatcggc aaccagacca
    27481 agatccttaa caaaaatatc gacggctgaa tgcggggtgt aatcgccatc caccacatga
    27541 cgcatccggt tttcgaacat ccaggaattt ccggcggcat tggtcacgac gtcatacatc
    27601 acatccagcg ggatccccgc acgggctgca agtgccatcg cttcggctcc ggcagcaata
    27661 tgtacgcccg ctaacaactg gtgaataatt tttacggtcg aacctagtcc cggttctgca
    27721 cctatgcgat aaacttttcc ggcaacggct tccagcacgg gtgccagtcg ttcaaaggca
    27781 atatcgctac cggaggccat gacagtcatt tcaccgttag cggcttttac tgcaccacca
    27841 gaaactggcg catccagcat ttccagatcg aatccagcca gagcggtagc aatttcttgc
    27901 gcatcagcac tagcgatagt ggaagaaacc attactgccg taccgggttt cagatgttgt
    27961 gcaacgcctg tttcaccaaa cagcacctgt ttaacctggg ccgcattgac caccagcacc
    28021 agcagagcgt ccagtttttc ggcaaacgtc gcggcgttat cagaaacccc gcaagcacct
    28081 gcctctttca acgtagcgca ggcattgctg ttcaggtctg cgccccaggt agaaagacct
    28141 gcgcggacac atgacagtgc tgctcccatt cccattgacc ctaagccaac gataccgaca
    28201 tgaaactcag atcccgtttt catctgctct ccttgttaat ttaagtgata ttttgtttga
    28261 tattgtgaat ataagcgctg gaagataacg atatggtgag ctgattcaca taaattaaca
    28321 ttgtgtgtta ttttatgtga actaagcgtt agttgcgccg cgcacgtttc gcaggcaaat
    28381 agcgtagaat gtcagcagga caaaggaagg agcaaaagtt gatacccgta gagcgtcgcc
    28441 aaatcatcct tgagatggta gctgaaaaag gcattgtcag tattgctgaa ctaacggaca
    28501 gaatgaatgt gtcacatatg accattcgtc gggatttaca aaaactggag cagcagggag
    28561 ccgttgtgct ggtgtccgga ggcgtccagt ctccgggacg cgtggcgcat gaaccttctc
    28621 atcaggtaaa aactgcgctg gcaatgacgc aaaaagcggc tattggcaag ctggcggcaa
    28681 gtcttgttca gccgggaagt tgtatctatc tggatgcggg aacgaccacg ttagcgatag
    28741 cacagcatct gattcacatg gagtcactga ctgtggtcac aaacgatttc gttattgcga
    28801 actacttgct cgacaacagt aattgcacaa ttattcacac tggcggtgca gtgtgtcggg
    28861 aaaaccgttc ctgtgtcggg gaagccgctg cgaccatgct gcgcagcctg atgattgatc
    28921 aggcttttat ttctgcatcg tcatggagtg tgcgggggat ttctacgcca gcggaagata
    28981 aagtcacggt gaaacgggcg attgccagtg ccagccgcca gcgagttttg gtctgtgatg
    29041 cgacgaaata tggtcaggtg gcgacatggc tggcgttacc attaagcgag tttgatcaga
    29101 ttatcacaga cgacggtttg ccggagagtg ccagtctcgc gctggcgaag caggatctct
    29161 ctttgctggt tgcgaaaaat gaataatggc ctgcaataac atttggttac tcatgcttca
    29221 cagaagaagc atgagactac tttattttat aaaatgacag ccgcccgctt ttcggcgtgc
    29281 cggtatcaat ataaatctgg ttagcgaacg tctgaatgtt atcaaacatc atatgtccaa
    29341 atataaaata atcagcgccg tttatttgtt gtaactcgcc attaagcgat ttctgcacac
    29401 gatcaacagg ccagagtaat tcgctctccg ctatttcttt accaaagaga tattcactcc
    29461 ccggataatc tgcatgtgcg atgacatatt ttatgttgtc gttagtgatt tcaataatat
    29521 gtggaaggtg atggaatttc agcaacagat ctattgcctc ttgttgctct gaatcattta
    29581 aatcgaaaaa ccagtcacca ccgctggcaa gccacatatt tccatcgcca gtttcgaatg
    29641 catccaacgc catcgcttcg tggttgcctt taaccgacgt aaaccagggt tggtttagca
    29701 ggcgcagcac gttaagactc ttcggcccac gatcaatatt atcgcctgtg gaaataagta
    29761 agtcggtttc agggtaaaaa gagagttgat gtaaacggga ttgtaataac tgatattcac
    29821 catgaatatc accaacgacc catatatggc gatagtgatg ggcattgatt ttttgatagc
    29881 gtgtagatgg catggtttta ccctgtaaaa taagctttcc tattatacag ggtattttta
    29941 tttgattcgt cagttgtcgt taatattccc gatagcaaaa gactatcggg aattgttatt
    30001 acaccagact cttcaagcga taaatccact ccagcgcctg acgcggagtc agtgaatccg
    30061 ggtcgagatt ttccagtgct tcgaccgcag gcgaagtttc ttctggcact gacagcaaag
    30121 acatctgcgt accatccact tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac
    30181 gcagtttttg ccgtgcgcgc ttaataacct ctttcggtac gcccgccaga gcagcaaccg
    30241 ccagaccgta gcttttgctc gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt
    30301 cgctgtgctc cagcgcatcg agatgtacgt tggcgacgcc ttccattttc tccggtagct
    30361 gggtcagctc gaaatagtgg gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat
    30421 tttctgcgca cgcccacgcc agcgacagac catcgtaggt ggacgttcca cgcccgatct
    30481 catccattaa caccagactg tattcggtgg cgttatgtaa aatattggcg gtttcagtca
    30541 tctccaccat aaaggttgag cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa
    30601 agatgcgatc gataggtcca atctcgactt tttgtgccgg tacatagctg ccgatgtagg
    30661 ccatcagcgc aatcagtgcg gtctggcgca tataggtact tttaccgccc atgttcgggc
    30721 cggtaataat caacatacgg cgctgcggcg acagattcag cgggttagcg ataaatggct
    30781 cattcagtac ttgttcaact accggatggc gaccttcggt aatgcgaatg cccggtttat
    30841 caatgaaggt cgggcaggtg tagttcaggg tataggcccg ttccgccagg ttaaccagca
    30901 cgtcgagttc cgccagcgcg ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca
    30961 ggtcgaacag ctcttcataa agctgttttt ccagtgccag tgctttgcct tttgaggtga
    31021 gaactttatc ttcgtactct tttagctctg gaatgatgta gcgctcggcg tttttcagcg
    31081 tctggcgacg catgtagttg atgggtgcca gatggctttg cccacggctg atttgaatgt
    31141 agtagccgtg caccgcatta aagccaactt tcagcgtgtc caggccggta cgttcacgct
    31201 cgcggacttc cagacgctcc agataatcgg tcgcgccgtc agccagcgcg cgccactcat
    31261 ccagctcttc gttatagccc gatgcgataa caccaccgtc gcgtaccagc accggcggtg
    31321 tgtcgatgat tgctcgctcc agcagatcgc gcagctcggc aaactcgccc atcttctcac
    31381 gtagcgcctg taccggtgca ctatcgacag tttctaactg cgcacgcagc tccggcagtt
    31441 gctggaaagc gtggcgcata cgggccagat cgcgtgggcg agcagttcgt aaagccagac
    31501 gtgccagaat acgttccagg tcgccgacct gacgcagtac cggctgcaac tcggcggtga
    31561 aatcctgcaa tgcgccaata gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg
    31621 gcatatgcag ccagcgtttc agcatacgac tacccatcgg cgtgacggtg cagtcgagca
    31681 cagaagccag cgtattttcc gcaccgcccg ccaggttctg agtaatttcc aggttacgac
    31741 gtgtcgcggc atccataatg atgctgtcct gctcacgttc catggtgata gaacgaatat
    31801 gcggcagggt cgtgcgttgg gtatctttcg catactgcaa cagacaaccg gcagcacaaa
    31861 gtccgcgcgg cgcgttctcg acgccaaaac cgaccagatc gcgggtgcca aattgcagat
    31921 tcaactgctg gcgcgcggtg tcgatttcaa actcccacag cgggcgacgg cgcaggccgc
    31981 gacggccttc aatcagcgac atctcggcga aatcttctgc atacagcagt tccgccggat
    32041 tagtgcgttg cagctctgcc gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac
    32101 gcccggagct gatatccagc gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg
    32161 ccagcaggtt gtcctgacgc tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa
    32221 cgatacgcac aactttgcgc tcaaccggac ctttgctggt cgccggatcg ccaatttgtt
    32281 cgcagatggc aacggactct ccctgattca ccagtttggc gagatagttt tccaccgcat
    32341 ggtagggaat ccccgccatc gggatcggct ctcccgccga agcaccgcgt ttggtcagtg
    32401 aaatatccag cagttgcgac gcgcgttttg cgtcgtcata aaacagttca taaaaatcac
    32461 ccatccggta aaacagcagg atctcgggat gctgggcttt cagcctgaga tactgctgca
    32521 tcatgggcgt atgggcgtcg aaattttcta ttgcactcat ggggttatgt ccggttccct
    32581 gatattaaat ggggtgtgat ggtttttatt ttcgttgtaa tcacataagg cgcacatgat
    32641 accggagtta atcatttcag gggaagaagc gaacggtgag ttcttgatgc tggttccggg
    32701 aaagatgctg ttttgcagct tttgcgtgat gcttatgaag caagtacgca aaattgcgtc
    32761 ggttggcgaa cctttcttct ttagagtcat agtgcccttt aagattaaga tgtaatataa
    32821 atacatctta atgaggtgga ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga
    32881 cgattaaaaa aatgatcgat ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg
    32941 agcattacga ggcgttgttt gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg
    33001 aagagaaacc agcctgtaag cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag
    33061 agatgaaaca gattatgcgt tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa
    33121 ccgtgcgtca tctcatcgat gacaagcgtc cggtgccaga actgccggaa aaatatcgtc
    33181 cgaaaaaacc tcgtgaatag tatgccggac aggcagatct gtccggcaat ttgcagttaa
    33241 atcaatgccg atttatcaat tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc
    33301 agccccagac gttgcgcagc gcctttaggc ccggcaacca cgccgttggt ttctttcagc
    33361 acgcgaacaa tcaactgata ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc
    33421 ggcgtttcag gttccggtaa aacaatatct ggcaatgaca gctgcagcac gttaccgcgt
    33481 gttagcaata ccgcgcgctc aatgacgttt tccagttcgc gtacgttacc cggccactcc
    33541 atgttgctca aggtgcgcag cgtctcggca ggaatgctgt cgatattgcg ccccagacga
    33601 cgggcaattt tgaaggtaaa ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt
    33661 agtggcggca ggtgaatcgg gaatacgttc aggcggtaat agagatcgct acggaactca
    33721 cggtcggcga ccattttttt cagatcgcgg ttagtcgcgg cgattagacg cacgtccgtc
    33781 tgaatgattt tgttgctgcc gagacgttca aactcctgtt cctgcaatac acgcagcaac
    33841 ttcggctgta actccagtgg catatcgccc acttcgtcga ggaacaggga gcttttatcc
    33901 gccagttcaa aacgaccaat gcgctgggcg ctggcaccgg taaaagcccc acgctcatga
    33961 ccaaacagat cgctttccag caatccggca ggcatcgccg cgcagttcat tttgaccatg
    34021 cggcgattat tacgcccact gagattatgg atcgcacggg caatcagctc tttacccgtg
    34081 ccagtttcac cgaggatcag cacggtactg tcactttgcg ccaccatttc aacttgttta
    34141 agcacgctgt acatggcttc gctgcggcca ataatctcgc caaattcact atcaacattg
    34201 ttgagctgct cggtcagggc gaggttttca tcaaccagcc gttctttcag acgatggatt
    34261 tcctgatagg cgagggcgtt atcgacagcg attgccacac gttcggcaat ctggcgcagt
    34321 aaattcagat tggtagtggt aaacactttc tcttcgcatt gcgccagttt cagcacgccc
    34381 agcatggtgt cgccagacat cagcggtaac aggcacaagg tttgaatctg gttgccccag
    34441 gtgtcgaaca acatgcgttc atagggggct aaatcgtccc gctcgtggag attgatcagc
    34501 agcatctctt tacttttgaa cacgcgttcg gtgagggttc cggcttcatc gacttcgctc
    34561 tgttcgtggg cgggatgctg tttatcaaga tagtgagtgg agtagatgtt gagtttgttt
    34621 ttacggtggc tgcgtaagac gatactgata tcgtcaatgt cgaaatagta atggatttct
    34681 ttggcgactt cgctgaccag ttcgtccata tccaggcggg aaagcaccgc gttggtgatg
    34741 gcgaccagga tgcggaagtt atcgcgttcc cggcataaca actcatagtc gacattgtta
    34801 acgacgcggc tctggatttg ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga
    34861 cgattgaact ctttttcgct ccaggggcga tcgtcataac gaataaattc acagccacca
    34921 aaaatatgcc cttccgccgc cagtggcatc aggcaatagt gaccaaattt tgggtatagc
    34981 ccacctgcgg ccagctgcgg ccaggtttca caaaattctt cgtaactgca atgcagcgta
    35041 tcagggcgcg acaaaatgct gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat
    35101 ttaatggggg tgtctttttc acgcgacgcg taataagacg cacgttgagt ctgcgcttgc
    35161 cacaacacaa tagccgcgtt gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc
    35221 tcacacagcg aggccagatc gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct
    35281 tgctgtccga gatcactcat cggtgtatat gacattttgt tcttccagga aagcaccgca
    35341 aagcggtgca aaaggtcatt gactaactat aggagattta gaattttatt agcatatacg
    35401 cggaagcggt tcggcgtgtg gtaaatcgag ggttcgtttc acaccataca gaccggcaag
    35461 acgaacacct ttacgttcca ccacttcacc aatcagcgcc gcatctttcc ccagtggatg
    35521 ggaatgtaac gctgccagca cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt
    35581 gccttcgttg gcaaagttca gggcgtccag tcccagcagt tcgcaaacgc cgcgcacggc
    35641 aggtttaacc ggcagcgccg cttctgaaat ttcaatacca caaccgcagg ctgccgcgaa
    35701 ctcatgaacc accgcgttta caccaccacg ggtggcatca cgcagcgctt tcacgccggg
    35761 aatgtcacgc agcgtctgaa taagcggcgt cagcaccgcg cagtcgctga ccagttcgcc
    35821 atccagcccc agctgctcac gcaggttaag gatagtcgcc ccgtggtcgc cgagcgtgcc
    35881 gctcaccagc aacatatcgc ctgcggttag cgtctgtgcg ccccaatgaa tattcgccgg
    35941 aattgcgccc atgccagcgg tgttgataaa cagtttatct gccgcgccgc gctgcaccac
    36001 tttagtatcg ccagtaacga tggcaatgcc tgccgtgcgg gcggtttctg ccatgctggt
    36061 tactacggct ttcagtgtct ccatcgacaa tccttcttcg aggataaagc cacaggagag
    36121 atagcgcgga atagcgccac tgaccgcaac gtcattcgcg gtgccgcaaa tcgccagctt
    36181 gccgatatta ccgccaggaa agaacagcgg gtcaatgacg taactgtcgg tggagaacgc
    36241 cagacgatcg ccttctgcta ccagctgcgc cagatcaaga cgtgcctggt cttcctgctc
    36301 tgccagccac gggttggcaa aggcttccat aaacaggctg ttgattaatt gctgcatcgc
    36361 ctggccgccg ctgccgtggg cgagttggat attattcacg cttcattctc ctgctgacga
    36421 tactgatacc acgcggcgca cgctccttcg gaggagacca tcagtgcacc aaatgcggtt
    36481 tgaggattac aggtgttacc aaacagcggg cattgatgcg gcttacattt gcccgttaat
    36541 acctcaccac aacgcgcgcg cgggtcatcg cagacctgct gcggtgccgg gcggaaatgt
    36601 gcttcggcat cgaatcgttg ataatccggc gtcaggtgca cgccagaaga ttcaatcacg
    36661 cctaagccgc gccattcgct gtcgccgttg acacagaaca catcggcaat cgcctgttgc
    36721 gccagcaggt taccggcatc cggtaccacc cgacgatact gattctctac cttgctgtgg
    36781 gccgctattt tctgctccac cagcatgacc acgccttgca gtagatcaag gggttcgaaa
    36841 ccagcgacca ccagcggacg atgaaaatcg ctggcgataa aattataggc atcagtgccg
    36901 ataaccatac taacgtggcc cggcgcgagg aacgcgtcga taccgttatc cggctgttcc
    36961 agcaaactgc gcagcgtcgg gataagcgta atgtgctggc agaagaagta aaaattctgc
    37021 acatcacgcg ctttcgcctg ttgcagagtg atggcggtgg tcggcatggt ggtttcaaaa
    37081 cctaagccga agaacaccac tttgcgggtt ggattctcct gcgccagttt caacgcatcc
    37141 atcggcgagt aaacgatgcg cacatcggca ccgcgtgctt ttgcctgcaa cagcgatccc
    37201 tgtttccctg gtacgcgcat ggcgtcgcca aaggtacaga agatgacttc cggatggctg
    37261 gcaatctcca cgcaggtgtc gattctaccc atcggcagta cgcacaccgg gcaacccgga
    37321 ccgtggataa actcaacgtt ttcaggcagt aactggtcga ggccgaattt aaaaatagcg
    37381 tgggtatgac caccgcacac ttccataatc cgcagagggc gttcggcggt gtaagagaga
    37441 tgtgaagcac gttcgcgcag atgctcaatt aactgcatca cctgttccgg cgcgcgatat
    37501 tcatcaacaa aacgcattat ttttcctcgc catacaacag cgcgccgaca tccggctcaa
    37561 cgtcaaacat gttttgtaag gcgtcgagag tgtcgcgtgc ttcggcttca ttaattacgc
    37621 tcatggcaaa gccaacgtgt accagtaccc actggcccac gcgcggctga ccgttttcat
    37681 cgcagctgcc gactaacgtt aaatcgacat cgcgctgaat gccgcagacg tcgacttttg
    37741 cctggttgcc gtcaatggtg cggatctggc cgggaacgcc tatgcacatc gctgtgtctc
    37801 cagccagttc agccactggt ccatcccttc accgctggtg gcggaaataa ggatgatttc
    37861 aatttctgga ttgacttcgc gggcgcaggc gatgcacttc tcaacgtcaa agttgagata
    37921 cggcaacagg tcaactttgt tgagcagcat cagcgaggcg gcggcaaaca tatgcggata
    37981 tttcagcggt ttgtcttcac cttcggtaac ggaaagcacc gccactttgt gtttttcacc
    38041 gagatcgaag ctggccgggc atacgaggtt gccaacgttt tcgataaaca gaataccgtt
    38101 atcgtccagt ggcagacgcg gtgcggcgtc ggcaatcatc tgtgcgtcaa gatggcagcc
    38161 tttacctgtg ttcacctgaa tcgctggtgt gccggtagcg cgaatgcgtg cggcatcgtt
    38221 cacggtttgc tggtcacctt caataaccgc gcacggaacg ctgtctttca ggcgcattag
    38281 ggtttccgtc agcagggtgg ttttaccgga acctgggctg gaaaccaggt tgagcaccag
    38341 ttgcttgcgg gcagcaaagc gcgcgcggtt gcgttcagcc agacggttgt ttttgtccag
    38401 cacgtcaatt tcgacttcca gcatccgacg ctggctcatg cccggtgcgt gagtgcccgc
    38461 ttcgccatga ccgtaatgca ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc
    38521 tttaatgccg gtgattttca tcttcgggcg tgccgccggg gcaaatggcg cgctacgaaa
    38581 cgcggaatga gggttatgtt catcaccctc gatatacagg ttgccttcac cgcaaccgca
    38641 tgttgtacac atcgctcact cctggtctat ttctatccgc cgaatctgta aaccgtcgtc
    38701 tgccacaatc tgtagcatgt caccatgaca ctgtggacag cggcggacgc gctgggtcaa
    38761 tagcgtcaca tactgttggc atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg
    38821 cagtttacaa ccttccgcca cgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga
    38881 gctggtttcg acacaagaaa atgcgccaat tttgagccag accccagtta cgcgttttgc
    38941 gccgtgtttt gcggcctgct gttcgatcaa ttccagtgcc cgttggcaga gggttatttc
    39001 gtgcatatcg cctcccatta actattgcca gctacaagca ataattgtgc cagtgttgat
    39061 tatccctgcg gtgaataatg tcgatgatgt cgaaataaca cgtcgacacg gcgacgaaat
    39121 tcatctttag cttaaaaatc tctttaataa caataaatta aaagttggca caaaaaatgc
    39181 ttaaagctgg catctctgtt aaacgggtaa cctgacaatg actatttggg aaataagcga
    39241 gaaagccgat tacatcgcac agcggcatcg tcgcctacag gaccagtggc acatctactg
    39301 caattcgctg gttcagggga tcacgttatc gaaagcgcgc ctgcatcacg ccatgagctg
    39361 cgcgccggac aaagaactct gtttcgtcct ttttgaacat tttcgtattt acgtcaccct
    39421 ggcggatggc tttaacagcc acaccatcga gtattacgtc gaaacaaaag atggcgaaga
    39481 caaacagcgg attgcgcagg cgcaactgag cattgacggc atgattgatg gcaaggtcaa
    39541 catccgcgat cgcgaacagg ttctggaaca ctatctcgaa aaaatcgctg gcgtttacga
    39601 cagcttatac accgccattg aaaacaatgt gccggtgaat ttaagccaac tggtaaaggg
    39661 acaaagcccg gcagcatgag ctgaggcttt gcccgttttg caggcgttac gcctgtttgg
    39721 ggatgggcgt gtcgatgagt gtcgaaaatg acatttcatc ggcatgtttt cgtcaaaaat
    39781 gacaatcacc tgaggaatgc ctggtgaatc gttttgtaat tgctgactcc acgctctgta
    39841 tcggctgcca cacttgtgag gccgcctgtt cagagacgca tcgccagcac ggcctgcaat
    39901 caatgccgcg cctgagagtg atgctaaatg aaaaagaatc tgcgccgcag ctctgtcacc
    39961 actgtgaaga tgcaccctgc gcggtggtct gcccggttaa cgccatcacc cgcgttgatg
    40021 gggccgtgca gttgaatgaa agcctgtgcg taagctgcaa gctgtgcggc atcgcctgcc
    40081 cgtttggcgc aattgaattt tccggcagcc gtccgctgga tattcctgca aatgccaata
    40141 ccccgaaagc gccaccggca ccgcctgctc cggcgcgtat cagtacgctg cttgactggg
    40201 tgccgggtat tcgcgcgatc gccgtcaaat gtgacctttg cagttttgat gaacaaggtc
    40261 cggcctgcgt gcggatgtgc ccgactaaag ccctgcatct ggtggataac accgatatcg
    40321 cccgcgtcag caaacgtaag cgtgagctga cctttaacac ggactttggc gatctcacct
    40381 tgtttcagca ggctcaaagt ggagaggcta aatgagcgca atttccctga tcaatagcgg
    40441 cgtggcatgg tttgtcgccg ccgctgttct ggcatttctc ttttcttttc aaaagacgct
    40501 aagtggctgg atagccggaa ttggcggcgc ggttggtagt ctgtatacgg cagccgcggg
    40561 cttcactgta ctgactggcg cggttggcgt gagcggtgcg ctgtcgctgg taagctacga
    40621 tgtgcaaatc tctccgctta acgcgatttg gctgattacg ctcggtctgt gcggtctgtt
    40681 tgtcagcctc tacaacattg actggcatcg ccacgcgcag gtgaagtgca acggcttgca
    40741 gatcaatatg ttgatggctg ccgccgtctg cgccgtcatc gccagcaacc tcggcatgtt
    40801 cgtggtaatg gctgaagtca tggccttgtg tgcggtgttc cttaccagca acagcaaaga
    40861 gggcaaactg tggtttgcgc tggggcgtct tggcactctg ctgctggcga ttgcttgctg
    40921 gctgctgtgg cagcgttatg gcacgctgga tctgcgcctg ctggatatgc gtatgcaaca
    40981 gctgccgctc ggttccgata tctggctgct cggtgtgatt ggctttggcc tgctggccgg
    41041 gattattccg ctgcacggct gggtgccgca ggcacatgct aacgcctctg cgccagctgc
    41101 cgcgttgttt tctacggtag tcatgaaaat tggcctgctg ggcattttaa ccctgtcgct
    41161 gctgggtggt aacgcgccgc tgtggtgggg gatcgcgctg ctggtgctcg gcatgatcac
    41221 cgcgttcgtc ggtggtctgt atgcgttgat ggaacacaac atccagcgcc tgctggctta
    41281 ccacaccctg gaaaatatcg gcattatcct gctggggctg ggcgctggcg taacgggtat
    41341 cgcgctcgaa caaccggcgc tgattgctct tggcctggtc ggtggtctgt accatctgct
    41401 taaccatagc ctgttcaaaa gcgtactgtt cctcggggcg gggagcgtct ggttccgtac
    41461 cggtcatcgc gatatcgaga aactcggtgg tattggcaag aaaatgccgg ttatctccat
    41521 cgccatgtta gtcgggttaa tggcaatggc tgcgctgccg ccgctgaacg ggtttgccgg
    41581 ggaatgggtt atctatcaat ccttcttcaa actgagcaat agtggcgcgt ttgttgcccg
    41641 tctgctgggg ccgctgctcg ctgtgggtct ggcaattacc ggtgcgctgg cggtgatgtg
    41701 tatggcgaaa gtctatggcg tcacgttcct cggcgcgccg cgtaccaaag aagccgaaaa
    41761 cgccacctgt gcgccgctcc tgatgagcgt aagcgtagtg gcactggcga tttgctgcgt
    41821 aattggcggt gttgctgcgc cgtggctgct gccgatgctc tctgctgctg tacctctgcc
    41881 tctggagcct gctaacacca ccgtttctca acccatgatc acattgctgc tgattgcctg
    41941 cccgctgctg ccattcatca ttatggcgat ttgcaaaggc gatcgtttgc cgtcgcgttc
    42001 ccgcggctcg gcctgggtgt gcggctacga ccacgaaaaa tcaatggtga ttaccgctca
    42061 cggttttgcc atgccggtga aacaggcgtt tgcgccggtg ctgaaactac gcaaatggct
    42121 gaatccggtg tctctggtgc cgggctggca gtgcgagggg agtgcgttgc tgttccgccg
    42181 gatggcgctg gttgaactgg cggtgctggt ggtgattatt gtttcacgag gagcctgaga
    42241 atgagtgttt tatatccgtt aattcaggcg ctggtgctat ttgccgttgc gccgctgctc
    42301 tccggtatta cccgcgtggc gcgagcccgc ttacataacc gtcgtgggcc gggtgtgttg
    42361 caggagtatc gtgacattat caaactgctg ggccgccaga gcgttggccc ggatgcctcc
    42421 ggctgggtgt tccgcctgac gccgtatgtg atggtgggcg tcatgctgac tatcgctact
    42481 gcgctgccgg tggtgaccgt cggttctccg ctgccgcaac tgggcgattt gatcacctta
    42541 ctgtatctct ttgccatcgc gcgtttcttc tttgccattt ccggtctgga taccggtagc
    42601 ccgtttactg ctatcggcgc gagccgtgaa gcgatgcttg gcgtgctggt cgaaccgatg
    42661 ctgctgcttg gtctgtgggt tgccgcacag gttgccggtt ccaccaacat cagcaacatc
    42721 accgacaccg tttatcactg gccgctgagc cagagcatcc cgctggtgct ggcgctttgt
    42781 gcctgtgcgt tcgccacttt tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc
    42841 gagcaggagt tgcaggaagg tccgctctcc gaatacagcg gtagcggctt tggcgtcatg
    42901 aaatggggta tcagcctgaa acagctggtg gtgttgcaga tgttcgtcgg ggtgtttatt
    42961 ccgtggggac aaatggaaac cttcaccgcc ggtggactgc tgctggcgct ggtgattgcc
    43021 atcgtcaaac tggtggtcgg cgtcctggtt atcgcgctgt tcgaaaacag catggcccgt
    43081 ctgcgtcttg atattactcc gcgcattacc tgggctgggt ttggctttgc atttttagcg
    43141 ttcgtctcct tgctggcggc gtgattaaag agagtttgag catgtctgaa gaaaaattag
    43201 gtcaacatta tctcgccgcg ctgaatgagg catttccggg cgtcgtgctg gaccacgcct
    43261 ggcagaccaa agatcagctg actgtcaccg taaaggtgaa ctacctgccg gaagtggtgg
    43321 agtttcttta ctacaaacag ggtggctggc tgtcggtgct gtttggtaac gacgaacgca
    43381 aactgaatgg tcattacgcc gtttattacg tgctgtcgat ggagaagggc actaagtgtt
    43441 gggtaacggt tcgcgtcgaa gttgacgcca acaaaccgga gtatccgtcc gtgacgccgc
    43501 gcgttccggc ggctgtgtgg ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg
    43561 ttggtctgcc ggatgaacgc cgtctggtgc tgccggatga ctggccggat gaactttatc
    43621 cgctgcgtaa agacagcatg gattatcgtc agcgtccggc gccgaccacc gatgctgaaa
    43681 cctacgagtt catcaacgaa ctgggcgaca agaaaaacaa cgtcgtgccg attggtccgc
    43741 tgcacgtcac ttctgacgaa ccgggccact tccgtctgtt cgtcgatggc gaaaacatta
    43801 tcgacgccga ctaccgtctg ttctacgttc atcgcggcat ggaaaaactg gcggaaaccc
    43861 gtatgggtta caacgaagtg accttcctct ctgaccgtgt gtgcgggatc tgcggctttg
    43921 cccacagcac cgcctacacc acgtcggtag aaaacgcgat gggtattcag gtgccagaac
    43981 gtgcgcagat gatccgcgcc attctgctgg aggtggaacg cctgcactcg catctgctca
    44041 acctcggtct cgcctgccac tttaccggct ttgactccgg ctttatgcag ttcttccgcg
    44101 tgcgtgaaac ctccatgaaa atggcagaga tcctcaccgg tgcgcgtaaa acctacggcc
    44161 tgaacctgat cggcgggatt cgtcgcgatc tgttgaaaga tgacatgatc cagacccgcc
    44221 agctggcgca acagatgcgt cgtgaagtgc aggagctggt ggatgtgctg ctgagtacgc
    44281 cgaacatgga acagcgcacc gtcggcattg gtcgtctgga cccggaaatc gcccgcgatt
    44341 tcagtaacgt tggcccgatg gttcgcgcca gcggtcacgc ccgcgatact cgcgccgatc
    44401 acccgtttgt cggttatggc ctgctgccaa tggaagtcca cagcgagcag ggctgcgacg
    44461 ttatttcccg tctgaaagtg cgtatcaacg aagtttatac cgcgctgaac atgatcgact
    44521 acggtctgga taacctgccg ggcggtccgc tgatggtgga aggctttacc tacattccgc
    44581 accgtttcgc gctgggcttt gccgaagcgc cgcgcggcga cgatatccac tggagcatga
    44641 ccggcgacaa ccagaagctg taccgctggc gctgccgtgc cgcgacctac gcgaactggc
    44701 cgaccctgcg ctacatgctg cgcggtaaca ccgtttccga tgcgccgctg attatcggta
    44761 gcctggaccc ttgctactcc tgtaccgacc gcatgaccgt ggtcgatgtg cgtaagaaaa
    44821 agagcaaagt ggtgccgtac aaagaactcg agcgttacag cattgagcgt aaaaactcgc
    44881 cgctgaaata aggaatcgcc atgtttacct ttatcaaaaa agtcatcaaa accggcacgg
    44941 cgacctcgtc ttatccgctg gagccgattg cggttgataa aaacttccgt ggtaagccag
    45001 agcagaaccc gcagcagtgc atcggctgcg cggcctgcgt caatgcctgc ccgtcaaacg
    45061 ccttaacggt tgaaactgac ctcgccacag gagagcttgc gtgggagttt aatctcgggc
    45121 gctgcatctt ctgtggacgc tgcgaagaag tctgcccgac ggcagcgatc aaactgtcgc
    45181 aagagtacga actggcggtg tggaagaaag aagatttcct gcaacagtcc cgttttgtgc
    45241 tgtgcagctg tcgcgtctgc aatcgtcctt tcgccgtcca gaaagagatc gactacgcca
    45301 ttgcgctgct taagcacaat ggtgacagcc gcgcggaaaa ccaccgcgaa agctttgaga
    45361 cttgcccgga atgtaagcgc cagaaatgcc tggtgccgtc cgaccgtatt gaactgactc
    45421 gccatatgaa agaggccatc tgatgagcaa tttattaggc ccccgtgacg ccaacggcat
    45481 tccggtcccg atgacggtgg atgaatccat cgccagcatg aaggcgtcgt tactgaaaaa
    45541 aatcaaacgt tctgcctacg tttaccgcgt ggactgcggc ggctgcaacg gctgcgaaat
    45601 cgaaattttc gccacgcttt cgccgctgtt tgatgcagaa cgcttcggca ttaaagtcgt
    45661 tccttcaccg cgtcatgcgg atattttact gtttaccggc gcggtcaccc gtgcaatgcg
    45721 atcccctgcg ctgcgtgcgt ggcagtccgc gccggacccg aaaatctgta tctcctacgg
    45781 tgcctgcggt aacagtggcg ggatcttcca cgatctctac tgcgtgtggg gcggtacgga
    45841 taaaatcgtc ccggtagatg tctacatccc tggttgcccg ccaacgcctg ccgccacgct
    45901 gtacggcttt gcaatggcgc tcggcctgct ggagcagaaa attcacgccc gtggcccggg
    45961 tgagcaggat gaacaaccgg cggaaatcct gcatggcgat atggtgcagc cgctgcgtgt
    46021 gaaagtggat cgcgaagcac gccgcctggc aggttatcgt tacggtcgtc agattgccga
    46081 tgacttcctg acacagttag ggcagggcga agaacaggtt gcacgctggc tggaagcgga
    46141 aaacgatccg cgtctgaacg agattgtcag ccatctgaat catgttgtag aagaggcgcg
    46201 tatccgatga gtgaaaaggt ggtgttcagt caactgagcc gtaaatttat tgatgagaac
    46261 gatgccacgc ccgccgaggc gcagcaggtg gtctattaca gcctggcgat tggtcaccac
    46321 cttggggtta tcgattgcct ggaagcggcg ctcacctgcc cgtgggatga atatctggca
    46381 tggattgcca ctctggaggc gggcagcgaa gcccgccgca aaatggaagg cgtgccgaaa
    46441 tatggtgaga tcgtcatcga cattaaccat gtgccgatgc tggccaacgc attcgataaa
    46501 gcccgggcag cgcaaacttc gcagcagcag gaatggagta caatgctgct aagtatgctg
    46561 catgatattc atcaggaaaa cgccatctat ttgatggtga ggagactgcg tgactgacgt
    46621 tttactctgt gttggcaata gcatgatggg cgatgatggc gcaggcccgc tgctggcgga
    46681 aaagtgcgcc gccgcgccga aaggtaactg ggtggtgatt gacggcggta gtgcaccgga
    46741 aaacgacatc gtcgccatcc gtgaactgcg ccctacgcga ctgctgattg tcgacgccac
    46801 tgatatgggg ctaaaccccg gcgagatccg catcatcgac ccggatgata tcgccgagat
    46861 gtttatgatg actacccata acatgccgtt gaactacctt atcgaccagt tgaaagaaga
    46921 tattggcgaa gtgattttcc tcggcatcca gccggatatc gtcggctttt actacccgat
    46981 gacccagccg attaaagatg cggttgaaac cgtttatcaa cgactggaag gctgggaagg
    47041 aaatggtgga tttgcgcagt tagcggtgga agtagagtag tttttcatta aggaattcag
    47101 ggaattgaac atcagaacag ggatgtgcgc ttttttattg agcttagtgc tgcctgcaca
    47161 ggccaccagc tttaccgaat atctgccgat gagtgacagc gaatacgcgc aaaagcgggc
    47221 gctgaaacct ctcctgacca tgccttatga tgccgagcaa aactggcatt ttagaaaagt
    47281 cggcgttgct ggcgtgacgc ttgaaaagat gccaaatgat gatagtgaat ggcaattgaa
    47341 cggtaaagat cgcgccggta aatcctggtc tgtgcctgtg ggggtgctgc aaaatatggc
    47401 gggtaatgcg caactttacc gtgccgatct tgatcgtaac ggcattcagg atctggttat
    47461 ctggcggggt atttcaggaa atggtctggc acctaacgcg tttttgattc tgatgacctt
    47521 taatcagcaa ggtcgcccgt gtgtttttca gtccgatggt ttttataccg caagtgaaac
    47581 tggcattgat gatttgctgg atttacaaag gaatggccac acgcaattgc tggatatgca
    47641 gtttgattcc ggttactgga taaccagtct gtatcaggta aaagacgcca aatggcagcg
    47701 tgttcacggc tggtttggca agttaagcta tcccgcgctc acgcgtttta cttatacccc
    47761 caaccgtaaa ctcgtcctca aacccatcgc cgggcgcgac ccgcaaacgg aagatctggc
    47821 gcagacgcag cgttgcttga taaaggggga tgtgctttat ggggtgaacc agaactgact
    47881 ttttgcagag tggctatgag ggggattacg tgcagacaga cgtcacgtta ctacagagag
    47941 aacaaagaat gactgctaat gctgcacgcg ctgttaaagc aacaagagaa ctggtcaatg
    48001 cagtcccctt tctgggaggc agtgactcag aagatgatta tcgtgaagca ctggaactgg
    48061 tcgaatattt gattgaagaa gatgacacca atccacttat cgactttctg gcgagccgca
    48121 ttgcagagta tgaaaacaac aacgaaaagt ttgcagagtt cgacaaagcc gttgcggcaa
    48181 tgccggttgg cgtggcgtta cttcgcacgt tgattgatca acataatctg acctatgcgg
    48241 atttgaagaa cgaaatcggc tctaaatccc tggtcagtca gattttgtct ggacaacgat
    48301 ccctgacaat atcccacatt aaggcacttt cagcccgatt tggcgttaag ccggaatggt
    48361 ttctttgatt ttgactgatg ccaaacccca gattgcccga tgcgctgcgc ttat
//
LOCUS       DAZFVO020000015        47736 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFVO020000015 DAZFVO020000000
VERSION     DAZFVO020000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.015"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            106..738
                     /gene="comR"
                     /locus_tag="ACHKNB_RS03940"
                     /old_locus_tag="pgaptmp_000788"
     CDS             106..738
                     /gene="comR"
                     /locus_tag="ACHKNB_RS03940"
                     /old_locus_tag="pgaptmp_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HGU4194355.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(800..1339)
                     /gene="ycfJ"
                     /locus_tag="ACHKNB_RS03945"
                     /old_locus_tag="pgaptmp_000789"
     CDS             complement(800..1339)
                     /gene="ycfJ"
                     /locus_tag="ACHKNB_RS03945"
                     /old_locus_tag="pgaptmp_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4194356.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1549..2853)
                     /gene="ndh"
                     /locus_tag="ACHKNB_RS03950"
                     /old_locus_tag="pgaptmp_000790"
     CDS             complement(1549..2853)
                     /gene="ndh"
                     /locus_tag="ACHKNB_RS03950"
                     /old_locus_tag="pgaptmp_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HGU4194357.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3261..3803)
                     /gene="ycfP"
                     /locus_tag="ACHKNB_RS03955"
                     /old_locus_tag="pgaptmp_000791"
     CDS             complement(3261..3803)
                     /gene="ycfP"
                     /locus_tag="ACHKNB_RS03955"
                     /old_locus_tag="pgaptmp_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HGU4194358.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3826..4851)
                     /gene="nagZ"
                     /locus_tag="ACHKNB_RS03960"
                     /old_locus_tag="pgaptmp_000792"
     CDS             complement(3826..4851)
                     /gene="nagZ"
                     /locus_tag="ACHKNB_RS03960"
                     /old_locus_tag="pgaptmp_000792"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGU4194359.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4862..5686)
                     /gene="thiK"
                     /locus_tag="ACHKNB_RS03965"
                     /old_locus_tag="pgaptmp_000793"
     CDS             complement(4862..5686)
                     /gene="thiK"
                     /locus_tag="ACHKNB_RS03965"
                     /old_locus_tag="pgaptmp_000793"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HGU4194360.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5667..6308)
                     /gene="lpoB"
                     /locus_tag="ACHKNB_RS03970"
                     /old_locus_tag="pgaptmp_000794"
     CDS             complement(5667..6308)
                     /gene="lpoB"
                     /locus_tag="ACHKNB_RS03970"
                     /old_locus_tag="pgaptmp_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HGU4194361.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6322..6699)
                     /gene="ycfL"
                     /locus_tag="ACHKNB_RS03975"
                     /old_locus_tag="pgaptmp_000795"
     CDS             complement(6322..6699)
                     /gene="ycfL"
                     /locus_tag="ACHKNB_RS03975"
                     /old_locus_tag="pgaptmp_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HGU4194362.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6702..7061)
                     /gene="hinT"
                     /locus_tag="ACHKNB_RS03980"
                     /old_locus_tag="pgaptmp_000796"
     CDS             complement(6702..7061)
                     /gene="hinT"
                     /locus_tag="ACHKNB_RS03980"
                     /old_locus_tag="pgaptmp_000796"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HGU4194363.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7395..9584
                     /gene="fhuE"
                     /locus_tag="ACHKNB_RS03985"
                     /old_locus_tag="pgaptmp_000797"
     CDS             7395..9584
                     /gene="fhuE"
                     /locus_tag="ACHKNB_RS03985"
                     /old_locus_tag="pgaptmp_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HGU4194364.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(9644..11077)
                     /gene="ptsG"
                     /locus_tag="ACHKNB_RS03990"
                     /old_locus_tag="pgaptmp_000798"
     CDS             complement(9644..11077)
                     /gene="ptsG"
                     /locus_tag="ACHKNB_RS03990"
                     /old_locus_tag="pgaptmp_000798"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HGU4194365.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11372..12169)
                     /gene="ycfH"
                     /locus_tag="ACHKNB_RS03995"
                     /old_locus_tag="pgaptmp_000799"
     CDS             complement(11372..12169)
                     /gene="ycfH"
                     /locus_tag="ACHKNB_RS03995"
                     /old_locus_tag="pgaptmp_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4194366.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12180..13184)
                     /gene="holB"
                     /locus_tag="ACHKNB_RS04000"
                     /old_locus_tag="pgaptmp_000800"
     CDS             complement(12180..13184)
                     /gene="holB"
                     /locus_tag="ACHKNB_RS04000"
                     /old_locus_tag="pgaptmp_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGU4194367.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13181..13822)
                     /gene="tmk"
                     /locus_tag="ACHKNB_RS04005"
                     /old_locus_tag="pgaptmp_000801"
     CDS             complement(13181..13822)
                     /gene="tmk"
                     /locus_tag="ACHKNB_RS04005"
                     /old_locus_tag="pgaptmp_000801"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4194368.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13812..14834)
                     /gene="yceG"
                     /locus_tag="ACHKNB_RS04010"
                     /old_locus_tag="pgaptmp_000802"
     CDS             complement(13812..14834)
                     /gene="yceG"
                     /locus_tag="ACHKNB_RS04010"
                     /old_locus_tag="pgaptmp_000802"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HGU4194369.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14837..15646)
                     /gene="pabC"
                     /locus_tag="ACHKNB_RS04015"
                     /old_locus_tag="pgaptmp_000803"
     CDS             complement(14837..15646)
                     /gene="pabC"
                     /locus_tag="ACHKNB_RS04015"
                     /old_locus_tag="pgaptmp_000803"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HGU4194370.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15766..17007)
                     /gene="fabF"
                     /locus_tag="ACHKNB_RS04020"
                     /old_locus_tag="pgaptmp_000804"
     CDS             complement(15766..17007)
                     /gene="fabF"
                     /locus_tag="ACHKNB_RS04020"
                     /old_locus_tag="pgaptmp_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGU4194371.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17095..17331)
                     /gene="acpP"
                     /locus_tag="ACHKNB_RS04025"
                     /old_locus_tag="pgaptmp_000805"
     CDS             complement(17095..17331)
                     /gene="acpP"
                     /locus_tag="ACHKNB_RS04025"
                     /old_locus_tag="pgaptmp_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4194372.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17542..18276)
                     /gene="fabG"
                     /locus_tag="ACHKNB_RS04030"
                     /old_locus_tag="pgaptmp_000806"
     CDS             complement(17542..18276)
                     /gene="fabG"
                     /locus_tag="ACHKNB_RS04030"
                     /old_locus_tag="pgaptmp_000806"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HGU4194373.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18289..19218)
                     /gene="fabD"
                     /locus_tag="ACHKNB_RS04035"
                     /old_locus_tag="pgaptmp_000807"
     CDS             complement(18289..19218)
                     /gene="fabD"
                     /locus_tag="ACHKNB_RS04035"
                     /old_locus_tag="pgaptmp_000807"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGU4194374.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19234..20187)
                     /gene="fabH"
                     /locus_tag="ACHKNB_RS04040"
                     /old_locus_tag="pgaptmp_000808"
     CDS             complement(19234..20187)
                     /gene="fabH"
                     /locus_tag="ACHKNB_RS04040"
                     /old_locus_tag="pgaptmp_000808"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGU4194375.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20255..21325)
                     /gene="plsX"
                     /locus_tag="ACHKNB_RS04045"
                     /old_locus_tag="pgaptmp_000809"
     CDS             complement(20255..21325)
                     /gene="plsX"
                     /locus_tag="ACHKNB_RS04045"
                     /old_locus_tag="pgaptmp_000809"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGU4194376.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21406..21579)
                     /gene="rpmF"
                     /locus_tag="ACHKNB_RS04050"
                     /old_locus_tag="pgaptmp_000810"
     CDS             complement(21406..21579)
                     /gene="rpmF"
                     /locus_tag="ACHKNB_RS04050"
                     /old_locus_tag="pgaptmp_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGU4194377.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21631..22152)
                     /gene="yceD"
                     /locus_tag="ACHKNB_RS04055"
                     /old_locus_tag="pgaptmp_000811"
     CDS             complement(21631..22152)
                     /gene="yceD"
                     /locus_tag="ACHKNB_RS04055"
                     /old_locus_tag="pgaptmp_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HGU4194378.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22351..22935
                     /gene="yceF"
                     /locus_tag="ACHKNB_RS04060"
                     /old_locus_tag="pgaptmp_000812"
     CDS             22351..22935
                     /gene="yceF"
                     /locus_tag="ACHKNB_RS04060"
                     /old_locus_tag="pgaptmp_000812"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HGU4194379.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23047..24006)
                     /gene="rluC"
                     /locus_tag="ACHKNB_RS04065"
                     /old_locus_tag="pgaptmp_000813"
     CDS             complement(23047..24006)
                     /gene="rluC"
                     /locus_tag="ACHKNB_RS04065"
                     /old_locus_tag="pgaptmp_000813"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HGU4194380.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24579..27764
                     /gene="rne"
                     /locus_tag="ACHKNB_RS04070"
                     /old_locus_tag="pgaptmp_000814"
     CDS             24579..27764
                     /gene="rne"
                     /locus_tag="ACHKNB_RS04070"
                     /old_locus_tag="pgaptmp_000814"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HGU4194381.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27961..28914)
                     /gene="flgL"
                     /locus_tag="ACHKNB_RS04075"
                     /old_locus_tag="pgaptmp_000815"
     CDS             complement(27961..28914)
                     /gene="flgL"
                     /locus_tag="ACHKNB_RS04075"
                     /old_locus_tag="pgaptmp_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HGU4194382.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28926..30569)
                     /gene="flgK"
                     /locus_tag="ACHKNB_RS04080"
                     /old_locus_tag="pgaptmp_000816"
     CDS             complement(28926..30569)
                     /gene="flgK"
                     /locus_tag="ACHKNB_RS04080"
                     /old_locus_tag="pgaptmp_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HGU4194383.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30635..31576)
                     /gene="flgJ"
                     /locus_tag="ACHKNB_RS04085"
                     /old_locus_tag="pgaptmp_000817"
     CDS             complement(30635..31576)
                     /gene="flgJ"
                     /locus_tag="ACHKNB_RS04085"
                     /old_locus_tag="pgaptmp_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HGU4194384.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31576..32673)
                     /gene="flgI"
                     /locus_tag="ACHKNB_RS04090"
                     /old_locus_tag="pgaptmp_000818"
     CDS             complement(31576..32673)
                     /gene="flgI"
                     /locus_tag="ACHKNB_RS04090"
                     /old_locus_tag="pgaptmp_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HGU4194385.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32685..33383)
                     /gene="flgH"
                     /locus_tag="ACHKNB_RS04095"
                     /old_locus_tag="pgaptmp_000819"
     CDS             complement(32685..33383)
                     /gene="flgH"
                     /locus_tag="ACHKNB_RS04095"
                     /old_locus_tag="pgaptmp_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HGU4194386.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQILVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33436..34218)
                     /gene="flgG"
                     /locus_tag="ACHKNB_RS04100"
                     /old_locus_tag="pgaptmp_000820"
     CDS             complement(33436..34218)
                     /gene="flgG"
                     /locus_tag="ACHKNB_RS04100"
                     /old_locus_tag="pgaptmp_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HGU4194387.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34356..35111)
                     /gene="flgF"
                     /locus_tag="ACHKNB_RS04105"
                     /old_locus_tag="pgaptmp_000821"
     CDS             complement(34356..35111)
                     /gene="flgF"
                     /locus_tag="ACHKNB_RS04105"
                     /old_locus_tag="pgaptmp_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HGU4194388.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35131..36336)
                     /gene="flgE"
                     /locus_tag="ACHKNB_RS04110"
                     /old_locus_tag="pgaptmp_000822"
     CDS             complement(35131..36336)
                     /gene="flgE"
                     /locus_tag="ACHKNB_RS04110"
                     /old_locus_tag="pgaptmp_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HGU4194389.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36361..37056)
                     /gene="flgD"
                     /locus_tag="ACHKNB_RS04115"
                     /old_locus_tag="pgaptmp_000823"
     CDS             complement(36361..37056)
                     /gene="flgD"
                     /locus_tag="ACHKNB_RS04115"
                     /old_locus_tag="pgaptmp_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HGU4194390.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNRGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37068..37472)
                     /gene="flgC"
                     /locus_tag="ACHKNB_RS04120"
                     /old_locus_tag="pgaptmp_000824"
     CDS             complement(37068..37472)
                     /gene="flgC"
                     /locus_tag="ACHKNB_RS04120"
                     /old_locus_tag="pgaptmp_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HGU4194391.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37476..37892)
                     /gene="flgB"
                     /locus_tag="ACHKNB_RS04125"
                     /old_locus_tag="pgaptmp_000825"
     CDS             complement(37476..37892)
                     /gene="flgB"
                     /locus_tag="ACHKNB_RS04125"
                     /old_locus_tag="pgaptmp_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HGU4194392.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38047..38706
                     /gene="flgA"
                     /locus_tag="ACHKNB_RS04130"
                     /old_locus_tag="pgaptmp_000826"
     CDS             38047..38706
                     /gene="flgA"
                     /locus_tag="ACHKNB_RS04130"
                     /old_locus_tag="pgaptmp_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HGU4194393.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38782..39075
                     /gene="flgM"
                     /locus_tag="ACHKNB_RS04135"
                     /old_locus_tag="pgaptmp_000827"
     CDS             38782..39075
                     /gene="flgM"
                     /locus_tag="ACHKNB_RS04135"
                     /old_locus_tag="pgaptmp_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HGU4194394.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39080..39496
                     /gene="flgN"
                     /locus_tag="ACHKNB_RS04140"
                     /old_locus_tag="pgaptmp_000828"
     CDS             39080..39496
                     /gene="flgN"
                     /locus_tag="ACHKNB_RS04140"
                     /old_locus_tag="pgaptmp_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HGU4194395.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANAQTSTTHRGGKKISI"
     gene            complement(39536..41071)
                     /gene="murJ"
                     /locus_tag="ACHKNB_RS04145"
                     /old_locus_tag="pgaptmp_000829"
     CDS             complement(39536..41071)
                     /gene="murJ"
                     /locus_tag="ACHKNB_RS04145"
                     /old_locus_tag="pgaptmp_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HGU4194396.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41181..42104)
                     /gene="yceM"
                     /locus_tag="ACHKNB_RS04150"
                     /old_locus_tag="pgaptmp_000830"
     CDS             complement(41181..42104)
                     /gene="yceM"
                     /locus_tag="ACHKNB_RS04150"
                     /old_locus_tag="pgaptmp_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4194397.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42106..42753)
                     /gene="yceH"
                     /locus_tag="ACHKNB_RS04155"
                     /old_locus_tag="pgaptmp_000831"
     CDS             complement(42106..42753)
                     /gene="yceH"
                     /locus_tag="ACHKNB_RS04155"
                     /old_locus_tag="pgaptmp_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HGU4194398.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42764..43348)
                     /gene="rimJ"
                     /locus_tag="ACHKNB_RS04160"
                     /old_locus_tag="pgaptmp_000832"
     CDS             complement(42764..43348)
                     /gene="rimJ"
                     /locus_tag="ACHKNB_RS04160"
                     /old_locus_tag="pgaptmp_000832"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4194399.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43584..44792
                     /gene="mdtH"
                     /locus_tag="ACHKNB_RS04165"
                     /old_locus_tag="pgaptmp_000833"
     CDS             43584..44792
                     /gene="mdtH"
                     /locus_tag="ACHKNB_RS04165"
                     /old_locus_tag="pgaptmp_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HGU4194400.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44856..45503
                     /gene="grxB"
                     /locus_tag="ACHKNB_RS04170"
                     /old_locus_tag="pgaptmp_000834"
     CDS             44856..45503
                     /gene="grxB"
                     /locus_tag="ACHKNB_RS04170"
                     /old_locus_tag="pgaptmp_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HGU4194401.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45637..46197
                     /gene="yceB"
                     /locus_tag="ACHKNB_RS04175"
                     /old_locus_tag="pgaptmp_000835"
     CDS             45637..46197
                     /gene="yceB"
                     /locus_tag="ACHKNB_RS04175"
                     /old_locus_tag="pgaptmp_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4194402.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46303..47349
                     /gene="pyrC"
                     /locus_tag="ACHKNB_RS04180"
                     /old_locus_tag="pgaptmp_000836"
     CDS             46303..47349
                     /gene="pyrC"
                     /locus_tag="ACHKNB_RS04180"
                     /old_locus_tag="pgaptmp_000836"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGU4194403.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47423..47668
                     /gene="dinI"
                     /locus_tag="ACHKNB_RS04185"
                     /old_locus_tag="pgaptmp_000837"
     CDS             47423..47668
                     /gene="dinI"
                     /locus_tag="ACHKNB_RS04185"
                     /old_locus_tag="pgaptmp_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HGU4194404.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11531 a        12894 c        11883 g        11428 t
ORIGIN      
        1 tcagttaaat agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat
       61 ttttataata acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca
      121 acacaatgtg taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt
      181 gataaggcca tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc
      241 gtcgaagcga ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg
      301 ttatttcgtg ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag
      361 ctgttttgtg aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc
      421 aactgcttta ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc
      481 ctctccccag attcaggaga tatcgccaat acgttgaaat cacgccatgc gatgcaagag
      541 cgcactttgc agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt
      601 gacgtgacac atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca
      661 cgcgaaggtg catcgctgga aaaactaatg cagattgccg gaacgacttt gcgtttatgg
      721 cccgaactgg tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa
      781 attgcagagt acagccttgt tatactttgt tattcaaaat cagttgccca ttactatcta
      841 gcgggatctg cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct
      901 tataggtcac atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct
      961 gttgcgtagt cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat
     1021 atccaccacc cagcgccccc acaacagtgg cgacatcttt accgcgacca ccgccaaact
     1081 gatgccctat cacgccgcca gcaacagcgc cgagcaccga cccggtaatg cgattttcat
     1141 cctgcaccgg tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa
     1201 ccgtttcctt gattggggtt gcagaaacaa cctgagcgta ttgcgggcca cgttcaaaca
     1261 cgttcagact ggccactgcc gctacgccca gcgcagctgc gacaccaatc ccgatacccg
     1321 ccaacattga tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc
     1381 gaaggagaaa agcagctatc agacagaaga tgaaaagcgc gggaatggct ggaaatactt
     1441 aaggattcgg aggagtctga aggaatgcct gatgcgacgc ggctgccgat cgcgctgttc
     1501 ctcaagggcg gataaggcgt ttatgccaca tccgccagtg tacgtcgatt aatgcaactt
     1561 caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa
     1621 gtaaccatgc agcgcaatct gatgcattcg gtatagcgag atatatacaa agcgcgcaat
     1681 tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac caacggtgga
     1741 gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt tcagcggctt
     1801 accattcatc tgcgccagaa tgttgttcat tgcgcaagtc gccatctggt gtgcagcctg
     1861 agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc
     1921 gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc accaccagct ggttgatacg
     1981 gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca
     2041 caccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc
     2101 actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc
     2161 cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt
     2221 tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc
     2281 gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt
     2341 cactttgcca tttgcgccca ggttggcgga gtatttcagg aagagattca gcatctcctg
     2401 gtgaaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacgcctgg
     2461 cgtattgaaa tcgttagagg tgctacccag cgccatgacc agggtgtcat aggcgatttt
     2521 acgttccgga accagcagtt cacctttctc gtcgcgtagt tctgcaatag tgattgtttt
     2581 cgcttcacga tcaatatcaa tgacggaacc caactggaac tggaaaccat gattgcgcgc
     2641 atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg
     2701 cagcagcggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt
     2761 cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc
     2821 gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa
     2881 ttgttaatta atggttatta acatagctct taattaacaa caggttacag caatgaaaat
     2941 gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat
     3001 caagttttgt tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa atagagttta
     3061 ttgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat atcgagggcg
     3121 tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg actgtcggat
     3181 gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac cggtaaggtt
     3241 acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg ctgtaaatgc
     3301 ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc gtaataatga
     3361 tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga caaaataacc
     3421 agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc aatatcggca
     3481 tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa attagggttg
     3541 aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta tccgcccagg
     3601 ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt gtccacttct
     3661 ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat caagcgtaca
     3721 tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact gttagagtca
     3781 aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt gacctgcttt
     3841 ctcttcctgc cagcgttcgt gtaactgatt cagacgggtg ctgatcgctt tccagcgagc
     3901 cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg taacacgttc
     3961 tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat tattgcagac
     4021 caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat aactgcccat
     4081 aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa aacccagttc
     4141 ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg gatcaacatc
     4201 actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga ttaaggaact
     4261 gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc acggtgtttc
     4321 tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg tcgttttcat
     4381 tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt tttgtggatc
     4441 ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca gcactggcgc
     4501 aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac ccgcctcttg
     4561 cgccagtttg ccgccctctt ccattcctga cagcgcagcg aatgattgcg ccgctggcaa
     4621 gcgggtaaaa ccttcacgaa aacgctgcac gcgtccacct tcctgatcta ccgccaccac
     4681 cagatgattg cgcgaagctg cgcggatctg gcgcaccagt tcacgtaact gggcaggatc
     4741 atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcaa gtatttcacg
     4801 ctcttccgcg tccagttcgt aacctttgac atccaacatt actggaccca cactgctctc
     4861 cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata aattgttgat
     4921 cgccggtttg tcgccagcgg tactcaaacc accctgcttt gagcatcagc agccagggaa
     4981 accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga gtggcatagt
     5041 cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc agttccagcg
     5101 cgatatcacc atctccggca tactcccagt cgatgagttt taaccctgac gcgctatgca
     5161 ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggc cgtggttccc
     5221 gcgctttgcg cagacgtttt aacattcgca gccaacccac tgtccgccgc gccggatcgc
     5281 tttgctgcca gtacagttcc agtaacggca acagcgttat tcgccagcca aaacgtggtt
     5341 gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa tacgttttta
     5401 ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc ggcttcggtg
     5461 caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg gactgcggcg
     5521 catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca atgagaaaac
     5581 tcccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg aaaaagcgcg
     5641 acagcaattc gtcgcgcatg atgggattat tgctgcgaaa cggcaccttt acctgaccag
     5701 ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc gttaacgttg
     5761 ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat gcctatggct
     5821 ttactacggg tacctaaact gtcctgcggc gacaaaccta actgttgctt agccatcgac
     5881 agctgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt tcgcagcgtt
     5941 tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct atcaaccagc
     6001 aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat cggctgcatt
     6061 gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgtttg atcttcgtgc
     6121 tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg gacaggctgt
     6181 tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc aggttcacgt
     6241 tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta gcgactcatt
     6301 tttgtcatca agattcaccc cttacaaata tagataaagt ctgactttgt gcgcccccag
     6361 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatgctaa cgctgcgtgg
     6421 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
     6481 ggtaacgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt gaatatcaga
     6541 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
     6601 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
     6661 caacgccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
     6721 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
     6781 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
     6841 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
     6901 acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt tggcgcttgc
     6961 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
     7021 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
     7081 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
     7141 taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
     7201 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
     7261 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttacaaa caaaattatt
     7321 cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg acaacataaa
     7381 ccaagagatt tcaaatactt tcaacacaat ttaacaggga taatcaatat caagccagca
     7441 ccaaaccatc actacttgcc ggttgcatag cattggcact attaccttct gccgcttttg
     7501 ccgctccagc cactgaagaa acggtgattg ttgagggttc agccacagct ccagatgatg
     7561 gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag atgactcaac
     7621 gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat cagcagttac
     7681 aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag gcggattccg
     7741 atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt gatggtatcc
     7801 ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg gcactctttg
     7861 aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt aatccatctg
     7921 cggcaattaa tatggttcga aaacacgcga ccagtcgtga atttaaaggc gatgtctcgg
     7981 cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc ccactcaccg
     8041 aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac tcatggctgg
     8101 accgctacaa cagtgaaaag accttcttct cgggcattgt cgatgctgat ttaggcgatc
     8161 ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc cctacctggg
     8221 gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgt gcacgcagta
     8281 ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg accctgaagc
     8341 agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa gtcgaatttg
     8401 acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg ctggttgggc
     8461 catacagtaa ttatggacct ggctttgatt atgtcggcgg caccggttgg aacagtggca
     8521 aacgtaaagt tgatgcgctg gatttgttcg ctgacggtat ttatgaattg tttggtcgtc
     8581 agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttac ttcagttcat
     8641 gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc aatttcccac
     8701 aaaccgactg gtcaccacag agcctggcgc aggacgatac cacacatatg aaatcgttat
     8761 atgctgccac tcgagtcacc cttgccgatc cgctgcatct gatcctcggc gcacgttata
     8821 ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc acgccttacg
     8881 ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat acctctattt
     8941 tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc accggtaaca
     9001 actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc acgttagcca
     9061 tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc cccggcagca
     9121 acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg gaatttgaac
     9181 tcaacggcgc aattaccgac aactggcagc tgacatttgg cgcaacgcgc tatattgcag
     9241 aggataacga aggaaacgcc gttaatccta atctgccacg caccacggtt aaaatgttca
     9301 ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt aactggcaaa
     9361 atcgcgtgta taccgacacc gtgacaccgt atggcacctt ccgcgccgag caaggtagct
     9421 acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg ttacagggga
     9481 acgtcaataa cctgttcgac aaaacctacg ataccaacgt ggaaggttct atcgtctacg
     9541 gcacaccgcg taatttcagc attaccggca cgtatcaatt ctgaataaca cctgtaaaaa
     9601 aggcagccat ctggctgcct tagtctcccc aacgtcttac ggattagtgg ttacggatgt
     9661 actcatccat ctcggttttc aggttatcgg atttagtacc gaaaatcgcc tgaacaccag
     9721 aaccagcaac cactacgccc gctgcgccca gtttcttcag gccagcctga tccactttag
     9781 acacatcagc aacgctgacg cgcagacggg taatacatgc gtcgaggtta gtaatgtttt
     9841 ctttaccacc aaatgcagca accagagccg gtgccatttc gctggtacct gtcgcttttg
     9901 catcttcagt cgcgtcttca cgacccggcg ttttcagatc cagtgcttta atcagcacgc
     9961 ggaagatggt gtagtaaaca atcgcataac cgataccgac gatcgggaac agccacagtt
    10021 tgctgctgtt accagacaga acgatgaagt cgatcagacc gtgcgagaac gacgtaccgt
    10081 cacgcatccc cagaagaata cagattggga atgccaggcc tgccagaatc gcgtggatga
    10141 tgtacaggat cggcgcaacg aacatgaagg agaactcgat cggctcggtg ataccggtca
    10201 ggaacgaggt cagcgccgcg gagatcataa taccgcccac tttcgcgcgg ttttctggtt
    10261 tagcagagtg ccagatagca attgcggcag ctggcagacc gtacattttg aacaggaagc
    10321 caccagacag tttacccgca gtcgggtcac ccgccatata acgcggaatg tcgccgtgga
    10381 aaacctgacc tgctgcgttg gtgtattcac caatctgcat ctggaaaggt acgttccaga
    10441 tgtggtgcag accaaacggt accaggcaac gttcgatgaa accgtaaatg ccaaacgcaa
    10501 ctaccgggtt ctggtaagca gcccactgag agaaggtctg gattgcagaa ccaatcggcg
    10561 gccaaatgaa ggacagcaca acgccagtaa agatggcagc caggccagaa atgatcggca
    10621 caaagcgttt accggcaaag aagccaagat actcaggcag cttaatacgg tagaaacggt
    10681 taaacatgta cgctgcgatc gcaccggaga taatccctcc gagtacgcca gtatccgcca
    10741 ggtgtttaga ggcgatttct tcagcaggta aatgcagtac cagtggcgca accacggcca
    10801 tggttttaac catgatgcca taggcaacaa ctgcggccag cgcggatacg ccatcgttat
    10861 tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg catgtttgca aagacggaac
    10921 cgcctgcttc tgccataaca tgcgatacaa cggcgggcag ccagctgaaa ttcgcggaac
    10981 cgacgcccag caggatacct gcgataggca gtacggatac cggcagcatc agcgatttac
    11041 cgaccttttg caggttagca aatgcattct taaacataat tgagagtgct cctgagtatg
    11101 ggtgcttttt ttacgttctc acgcgtggca aggggggaga gcctcgccgt gtacagggca
    11161 tctaagcgcc ctttatttat tacacagagt aaaataattt agtgccaatt tgtttgacgg
    11221 ctatcacgtt tcaacttcag atcattggag aagttgcaca ttttgacaaa acatatgtca
    11281 caatatttca aacgtggtta ttcaccgccc aattttcggc ggtgaacttt actcgtttta
    11341 gccattaatt acgagcttta aaaaaactca ttcaacggat ggattgaagg cgggaagcgt
    11401 cgatgtgaaa cagacgggcg aagttatcgg tggttacctg cgccagttct tcaacggcaa
    11461 cacctttcaa cacagccatg tattctgcaa cgtcacgaac catcgcaggt tgattctctt
    11521 ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt ttccaccagt aatcgatcca
    11581 gggggacata acgcgcagca tcgcgcagtt gctccgcatt acggaaggtc acaatgccgg
    11641 aaaaggagat gtaaaatccg agatccagta atttacccgc cgtttctctg tcctctgtaa
    11701 aacagtgtag tacgccaccg caatccgtca ctttttcttc gcgcagaatt gccagcgtat
    11761 cggcacgggc gtcacgggta tggacgataa ccggcttgtt cagttcacga ccaatctgga
    11821 tatgatggat gaaggactct tgctgacgta ctttagtttc cggtgtgtaa taataatcca
    11881 gcccggtttc acccagcgct acaacaccct cttctgccgc cagacggcgt aaatcttcta
    11941 catcgtaggg atcattctgg ttgagtggat gcacgccaca agaaaatacg acgttgtcac
    12001 gttcgcctac cagatcccgc atatgtaaat aacccggtaa tgttgtggcg actgccagac
    12061 aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc atccacgtcc ttatgcaaag
    12121 attcataatc cagaccatcg agatggcagt gtgagtcgac taaaaacatg atgtctctct
    12181 taaagatgag gaaccggtag cacaacgccc ggttgcaggt aatgctcaat acgcagtaaa
    12241 agatcggtga tgagaagctc gcggttgatg cctgtaacag acattaactg ttcacgaatg
    12301 tggcaaacat cccccagtat agcctgcagg cgcgagggag aaagatggtt tgccagttcg
    12361 gcgaccaggc ccggcacatc aacattggtc acctgcgcag caccatgatg gcgttttagc
    12421 gcatccatca gcaacgttgc cagccagtgt aaacgcgccg gagcttgttc atgattaagg
    12481 gccgctagca gcgaatacca gtcgcccgat ggcacgctat atgccaacgc ctgacacaat
    12541 gtttcacgag cctgccagtt atctccctga aacaacgcca gtgccgcgcc aggcgaaccg
    12601 gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca ttgtcacttc gcgtgaaagc
    12661 caggtcacgg cgtactgttc cggcggcggc gcaaggtaat gtaaccgaca acgactacgt
    12721 aatgttgcca gtaaacgttc aggctcgcgg gtagccagga aaaaccaggt ttctgctggt
    12781 ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt cggtcagtaa ggcagcatcg
    12841 gttacccaga caactttcgc accacctagg cgtgcgtgct cattcagctt ttcggtgacc
    12901 tcacgtaccg catcaatgcc cagcatattt ttcccttttt cgggagccag ggtgtagtaa
    12961 tcgggatgcg tgccagcctg catcaactga catccacgac agtgaccgca acttttgtgg
    13021 ccctgcggtt gttggcagag taaataacgg ctcagggcgt agattaaagc atcatcgccc
    13081 atgcccggta acgcctgaat gagtagcgca tggtgacctc ttccggcctg atagctggct
    13141 accagttttt cgaaatcagg tcgtaaccat ggataccatc tcatgcgtcc aactccttaa
    13201 cccagtgggt cacggtagtg cggattgcat ccatcacggc ctccagcggc tgggtggcat
    13261 caatggtatg aatactttta tcttgtgcag ccagttccag atagcgggcg cgggtgcgat
    13321 taaagaaatc gaaagactct tgctcaatac gatccagctc gccgcgcgca cgcgcgcgtt
    13381 ttaagccaac ttccggggta acatcgagat agagcgttaa gtcggggcga aaatccccga
    13441 gaacagcatc acgcagtgtt gccagcatat gttggtcaat accacgtcct ccgccctgat
    13501 acgcctgagt ggagagatcg tggcgatcgc caatcaccca ggtgccgtta gccagagctg
    13561 gtttgatgac cgtttctacc agttgaacgc gcgcggcata aaacatcaga acttcggctt
    13621 tatcggtaat gacttcatcg cctaccgatt tgatatccag caccaggctt cttaactttt
    13681 cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat gtcgcggata cccagttgct
    13741 caagcgtctc aaccaccaca ttacgcgcgg tagttttgcc tgcgccttcc agcccctcaa
    13801 tgacgatata cttactgcgc atttttttcc ttaagcactt tcagataatc ctgcacagac
    13861 ttgttatgac tggcaagatt ggtattaaac gtgtgaccac ctttaccatc ggccacaaaa
    13921 tagagatacg gcgtttttgc cggatgcgca gcagccttca gcgaatccgc ccccggcgta
    13981 gctatcgcac ccggcggcaa accggtaatg gtataggtgt tatacgctgt cggcgtttcc
    14041 aggtctgcac gagaaagttt gccattataa cgctctccca tcccgtaaat cacggttggg
    14101 tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa ataccgaggc aacctgatcg
    14161 cgttcactgg caacggcggt ttctttttcg ataattgatg ccatcgtcac cagctgattt
    14221 ttatctttat aaggcagacc gtccgcacgc ccttcccagg cgctatcgac cgctttcacc
    14281 attttcttgt gcgctcgctt gagtaacgcg acatcggtgg tattggcggt atacatccag
    14341 gtgtctggcc agaaccaacc ttcaatccac tccgggtttt ccagttcaag tgcctgcgct
    14401 acggtggcgt acttatcgtc actcagcgtg tgcttgatat acggagcctc acgcaattgc
    14461 ttgaggtagt cgctcagacg catcccttct accagtcgca gcgggaactg tgcttcttta
    14521 ccgctttcca acaatttcag catctcgcgc acggtcatct gcggtgtaaa gcggtaagtc
    14581 ccggctttaa agtgagaaag atccggttcg atacgcagca gccattgaaa aacccgtggg
    14641 cgattgatga tcttatcagc ataaagctgt tcaccgagcg ccagacgtcc ggtccctggc
    14701 ttcagggtaa atatcgtctc ttctttgata agcaatttgc tgtcggcaag atggcgaacc
    14761 ttccagacgc ccacaccagc ggcgataccc agtaccacca gcaataacaa gattatcaat
    14821 aacacttttt tcatgactaa ttcgggcgct cacaaagtgg ggctaaatat tcatataacg
    14881 ttgctgacga aaaggagaca tcgccacagg cacgtacggg catcactggc attaacgcat
    14941 tacaaataac catctcatct gcctgcaacg cctcttccag agaagcttgc acttcgacaa
    15001 gctgataaga ggattgtgcc agcaaacgga tacagaattg tcgcataatg ccgttaacac
    15061 ctgcctgatc cagtcgcggc gtataaacta cgttgccctt ccgccagaac aaattagccg
    15121 cacagcattc cgtaacccac ccttcgctgt caaggaccag cgcctcatca gcgtttgtct
    15181 gctcaagatg agagcgaatc aatacttgct caagacgatt gagatgttta atacctgcaa
    15241 gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa cgtcatcccc tcgttacgca
    15301 aacggtcgta atgtgcagga taagccgtaa cggagagaat ccgcgttgcc ggtccactgt
    15361 tcaatgtgct gtaccctcgc ccgccactac cgcgactgat cacgactttc agtacaccat
    15421 tttgctgttc tgctgccagc gttttcatct cttgttcaag ctgtggccag aagtcacagg
    15481 aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat atgcgccgat aacaaactga
    15541 ctttaccgtc gataactctg gcggtggtaa aacaaccatc accaaactgc gttgcccgat
    15601 cgcttactgc cagcgattcc tgcttataac cgttaattaa gaacataccg gctccttatc
    15661 agagaattgt tagtgtggca gcatgttcac tacggaacaa gtcggaataa aagctaagaa
    15721 aaaaggcccg caagcggacc ttttatatgg gtggaaaatg acaacttaga tctttttaaa
    15781 gatcaaagaa ccattagtgc caccgaagcc gaaggagtta cacagcgtgt attccattcc
    15841 gctgacctga cgtgcttcat gcggtacgaa atccagatcg caaccttcat ccgggttatc
    15901 caggttgatg gttggcggaa cagcctgatc gcgcagtgcc aggatggagt agatagattc
    15961 tactgcaccc gccgcaccta acaggtgacc ggtcatagat ttcgtggagc ttaccaacac
    16021 acggcttgca gcttcaccga agatggtttt caccgcctgc gcttcagctt tatcgccagc
    16081 cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga ctcgcttcaa tgcctgcatc
    16141 acgcagagca tttgccattg ccagagctgc gcctgcgcca ttttctggcg gtgacgtcat
    16201 atgataagca tcgctgctca taccaaagcc gacgagttca gcgtaaattt tcgcaccgcg
    16261 ttttttcgcg tgttcgtact cttcaagtac cagcataccg gcaccatcgc ccagtacgaa
    16321 gccatcacgc tctttatccc acgggcggct cgccgcttgc gggttatcat tgcgggtaga
    16381 taatgcacgt gccgcgccaa aaccaccaac acccagcggc gtactggctt tctctgcgcc
    16441 acctgcaacc atcacgtcag catcgccata cgcgataata cgcgcagcat gaccaatgtt
    16501 gtgcacgccg gaagtacagg cggtcgcgat agagatgctt gggccacgca ggccatacat
    16561 gatagtcaga tgacctgcca ccatgttcac aatcgttgac ggaacgaaga atgggctgat
    16621 cttacgtgga ccaccgttca tcagagatgt gtggttttct tcgatcagtc cgaggccgcc
    16681 aatcccggag ccaattgcgg caccaatgcg ggttgcgttc tcttccgtta tttcaaggcc
    16741 agaatcctgc atggcctgaa cgccagcgac aattccatat tgaatgaagg catccatctt
    16801 gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa tcctttacta agccagcaaa
    16861 tttcgttgca taggcgctag tatcgaaatg gtcgattagg ctgatgccac tctgaccggc
    16921 aagcagagct ttccaggtag actctacggt attgccgaca ggagacaaca tgcccagtcc
    16981 ggtcacaact acacgacgct tagacacgtt tgtcctccag ggagggaaaa aatgattcta
    17041 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    17101 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    17161 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    17221 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    17281 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    17341 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    17401 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    17461 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    17521 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    17581 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    17641 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    17701 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca accacgttta cagtaatacc
    17761 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    17821 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    17881 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    17941 tgaaaggttg gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    18001 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    18061 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    18121 gttagcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat
    18181 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    18241 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    18301 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    18361 tttcgtcagg ccagttagca ctttgcccgg gccgacttca tagagatgtt ctacgccttg
    18421 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    18481 tgcgtcacga atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacagg
    18541 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    18601 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagagcgc gttttgcgcc
    18661 cgcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    18721 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcgg cttcttcaca
    18781 cgctttcgca atagacgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    18841 tggtacggct tcttgcatga acttgccgcg catctcaacc agacgcaccg catcagcgaa
    18901 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    18961 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    19021 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct gctgggtcag
    19081 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    19141 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    19201 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cggagcccca
    19261 ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    19321 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    19381 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    19441 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    19501 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    19561 gcccgccatt gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    19621 cagcaattca ccataactac cgtcggcatg cagatgggtg gagatgattc ccggctcttc
    19681 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    19741 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    19801 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cgcaggctgc
    19861 tgcaacgtca aatgccgggc aacctttaat gcccaacatg ctttgaatct gacaagctgc
    19921 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    19981 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    20041 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    20101 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    20161 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    20221 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    20281 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    20341 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    20401 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    20461 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    20521 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    20581 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    20641 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    20701 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcgaga ccctttactt
    20761 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    20821 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    20881 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    20941 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    21001 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    21061 tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca tcactggcga
    21121 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    21181 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    21241 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    21301 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    21361 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    21421 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    21481 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    21541 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    21601 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    21661 aaatgggttt ggcttttgcg cttcttcagg cagttcacca aagaccatgt ccgcttcgga
    21721 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    21781 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    21841 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    21901 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    21961 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    22021 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    22081 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt
    22141 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    22201 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    22261 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    22321 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacttcg
    22381 ccctggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcaccagag
    22441 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    22501 gaaaaagcac aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    22561 gtatgtgttc ttgatggcga aatcaccgga aaaccgttaa cggaagaaaa tgcccgtctg
    22621 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    22681 tcggcgaatg ggcatctgca aacagaagtg gagccttttg acgtccattt ccgtcacctg
    22741 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    22801 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagaggggcg cgaccctaac
    22861 acactggttg gtttaccgct aattgcgctg tgtcagatgt tacgtcggga agggaaaaat
    22921 ccgctgatgg gatagttttt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    22981 ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    23041 cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg
    23101 cgcttcgatg cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    23161 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    23221 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    23281 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    23341 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    23401 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    23461 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    23521 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    23581 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    23641 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    23701 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    23761 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    23821 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    23881 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    23941 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    24001 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctacgctt
    24061 atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    24121 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    24181 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    24241 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    24301 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    24361 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    24421 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    24481 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    24541 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    24601 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg
    24661 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    24721 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    24781 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    24841 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    24901 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    24961 ttctgatgcc gaacaacccg cgcgcgggtg gcatttctcg ccgtatcgaa ggcgacgacc
    25021 gtaccgaatt aaaagaagca ctggcgagcc ttgaactgcc ggaaggcatg gggcttatcg
    25081 tgcgcaccgc tggcgtcggc aaatctgctg aggcgctgca atgggattta agcttccgtc
    25141 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    25201 atcaggagag caacgtaatc gttcgcgcat tccgcgatta cttacgtcag gacatcggcg
    25261 aaatccttat cgataacccg aaagtgctcg aactggcacg tcagcatatc gctgcattag
    25321 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    25381 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccgtccg
    25441 gtggttccat tgttatcgac agcaccgaag cgttaaccgc catcgacatc aactccgcac
    25501 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagctgccg
    25561 atgagattgc tcgtcagcta cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    25621 tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    25681 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    25741 ctcgtcagcg cctgagccca tcactgggtg aatccagtca tcacgtttgt ccgcgttgtt
    25801 ctggtactgg caccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtctgatcg
    25861 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    25921 cttcttacct gctgaatgaa aaacgttctg cggtaaatgc cattgaaact cgtcaggacg
    25981 gtgtgcgctg tgtaattgtg ccaaacgatc agatggaaac cccgcactac cacgtgctgc
    26041 gcgtgcgtaa aggggaagaa accccaacct taagctacat gctgccgaag ctgcatgaag
    26101 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtccg gaacaacctg
    26161 cgctggcaac ctttgccatg ccggatgtgc cgccagcgcc aacgccagct gaacctgccg
    26221 cgcctgttgt agccccagca ccgaaagctg caccggcaac accagcaact cctgcacaac
    26281 ctgggctgtt gagccgcttc ttcggcgcac tgaaagcgct gttcagcggt agtgaagaaa
    26341 ccaaaccgac cgagcaacca gcaccgaaag cagaagcgaa accggaacgt caacaggatc
    26401 gtcgcaagcc tcgtcagaac aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    26461 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    26521 agcagactgc cgagacgcgt gagagccgtc agcaagctga ggtaacggaa aaagcacgta
    26581 ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    26641 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    26701 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    26761 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    26821 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    26881 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    26941 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    27001 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    27061 tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa
    27121 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    27181 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    27241 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    27301 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    27361 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccggtgg
    27421 ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    27481 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    27541 aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    27601 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    27661 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    27721 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    27781 atcaagccct ggtaactgcc agggcttttt tatttcatct ttgaatcaac atattaaaat
    27841 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    27901 ggcgatccca gagcaggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    27961 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    28021 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    28081 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    28141 ctccagttcg ttcagctgcg tgcctaattc cgcgcgaaca gtcagcacat tgttcagcga
    28201 gtttttcagt ccgcggttgg ttttatctaa cgccgcagcg gcggtttctt tatccgcttc
    28261 gctatccgcg accggcgttt tcagggctgc gatggcacta tccagcatgg caaaaagatt
    28321 ggtttcagaa gcgctaccgt ctggttccgc taccgcgttg ctggtaatac tgtcgaaaat
    28381 tttgtcaccc gtgtgcccta tcaccatcga acgcgaagca tcgacctgtt gtttaatact
    28441 ttctgctcca ccgacgtatt tccctttctc ttcgctaaac ggcgcagtct ctgttttata
    28501 accggcaaaa atgtagcgcc cgttaccgtc agtggtgttc gccagattca gcaactggtc
    28561 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    28621 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    28681 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    28741 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    28801 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    28861 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    28921 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    28981 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg
    29041 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    29101 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    29161 tcgttaaagg atttcgcacc acccaccgtt ttactgttgc tttgcagatc cagcagggcc
    29221 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct
    29281 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    29341 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    29401 ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    29461 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    29521 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg
    29581 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg
    29641 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    29701 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    29761 atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg
    29821 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    29881 gccatcgtga tgttataagt gccgccatcc tgaacgctga cttctacacc aacaatctgg
    29941 tttaattcgc tcaccagttg atcgcgttga tccagcagat tgttaggtga cgcccctgcc
    30001 cccacgcctg tcaggcggga gatttgatcg ttcaggctgg cgatttgttt agcgtaattg
    30061 ttgatctgat caacgctggc acctatcgcg atattgacct gtttgtcctg gtcgcgcaga
    30121 tattgatcgg tggttttaaa ctgattcacc aacccttctg atttcccaat cagcgcctgg
    30181 cgcgctgccg ggtcttccgc gttactcacc agcgtttgca ggctggtgaa gaaatcctgc
    30241 atctgtgttg ccagcgaaga ggtactggtg gagagcatat tgtcgatttt cgacatctgc
    30301 tcatagcggg cagtcaggcc gctactttgc gtctgcgccg cacgtaactg gttggtaata
    30361 aaagcatcat actcacgctg cacaccagaa acgtagacac cattgccaac ccagccgcca
    30421 gcgcccaacg tgctattggc ctgcgccata atagtggttt ggcgggtata tccggcaacg
    30481 ttatagctgg agatattatt acttgccgta tttaacgccg cctgggccgc gttcagtccg
    30541 ctcatggcgt tattaatcaa gctggacatg gaggttcctt ataagccttc aattacagag
    30601 tattatcggc agcgacccgc cggacttgag ttattcagaa cagattatca atgttcatgc
    30661 tgtaggtttt gctcaccttg tcgcttatcg atttcatctg ctgaatcatg ttggtgagtt
    30721 tgcgggcata gtgaggatcg gtggcatagc ccgcgtcctg tagggcctgc gccccctgtt
    30781 ccgcactcgc ggcggtcgtc acggcggcgt agcgcgggtt acgcgttaac agcccaacgt
    30841 aatccgacaa ggcttccaga tacgagctgt agacgcgaaa cttcgctttt actttcttcg
    30901 cttcgccgtt ttcatattca gtcgtggtga tttcagtaac tggccctttc cagttgccag
    30961 aggctttgac accaaacagg ttatagctcg gctcgccgtt ttcgcggcgg atttgccgtt
    31021 gcccccaacc agattccagt gccgcctgag cgaggatcaa atgatgtggc acaccgcttt
    31081 gctggcttgc cagttgggcg ggcagcgaga gttgcgcgag gaatgcttta ctgtcacccg
    31141 gcagcgaatc atcgtagtta cgtggcacgg ccttttgcac cagctgcgaa agcgcctgat
    31201 tttgataacg caccacggtt tcgagcggga atttcatcgg tgctgctggc gtggactcct
    31261 ctggcaatgg ttgttctggc gtcatctgtt taaccatcat ctctgcaagc cccagacctt
    31321 tgcccgccgt catctgttgg gcaatttgct ggtcatacat actggtatac agacgagtgt
    31381 gctcgctgct gaacaggcca tcttttggta aagcgtcgcg catgcttttc aacatcatct
    31441 gcacgaacat cccttccacc tggcgggcca ccggacggat atttgccgcc ggatcttcgc
    31501 ccgctttcgc ctttagttcg ttgagtgatt gcgcatccca ggccgcactt gccagtagtt
    31561 tgctgtcgct gatcatcaga tgatttccag ttttgcccgc agacatcccg cactttgcat
    31621 tgattgcaaa atagacatca gatccatcgg cgtagcgccc agcgcattga gcgcgcgcac
    31681 cacgttattg aggctggcgc tggaacgtac gctttgcagc gaaccgccgc tctggcgtaa
    31741 atcgatctgc gtttgtggag taaccacagt ctgtccacca ccaaacggtg tatctggctg
    31801 gctgacattg gcctgacgat tgactgttac tgagagattt ccctgcgcta ccgcgcagct
    31861 gtcgagggtc acttcgcgat tcatcaccac cgaaccggtg cgcgagttaa tcactacttt
    31921 agcgtcctgc ggggtgacat taacctgcat attctggata tctgcaagga aacggacctg
    31981 ggaactgttg ccacttggta cgcgcacctg aatagtccgc gcatctaacg cggtggcgct
    32041 gccatatcca cgcacgcgat tgatggtgtc agcgatttgc tgcgccatgc tgaaatcttc
    32101 gtcgttaagt tgcaaattaa gggtattccc gacgccaaac tggctgggca attcacgttc
    32161 aataaccgca ccattggtga tccgtccacc gttcagttgg ttcacctgaa cactgctacc
    32221 gccagcggag gctcctgcgc cgccaaccag aatattgccc tgcgccagcg catacacctg
    32281 actgtcaacg cccttaagcg gcgtcatcaa caacgtacct ccacgcaagc ttttggcatt
    32341 tcccatggaa gaaaccacca catcgatggt ttgcccctga cgtccaaacg gaggaagtga
    32401 cgctgtcacc attaccgcag cgacgttttt tagctgcata ttggtgcccg tcggaacggt
    32461 aattcccagc tgtgagagca tgttattaag cgtttgtgtg gtaaacggcg tctgggttgt
    32521 ctggtcaccg gtgccatcca gccccaccac cagaccatag ccaatcagtg agttttgcct
    32581 taccccctga acactggtga gatcgcgaat acgctcagcc tgagccgccg tcgtaaccag
    32641 tagaagaatt aatgcagaga gaaatttaat caccacagcc tcacttacat tggcgacagg
    32701 ttaaggaaga aacgctgcaa ccagcccata ttttgcgctt cgttaatgta gccattgcct
    32761 acgtattcaa tgcgcgcatc cgccacctga gtagacggta cggtattgct gccgctgata
    32821 gtgcgtggat taaccacgcc agagaagcga ataaattcgg taccctgatt aatggcaatc
    32881 tgtttttcac ccaccacatg caggttgccg ttgaccagta tctggtcaac cgtcaccgtc
    32941 aacgtgccgc taaaggtatt gctggcattg gccccgccct ttccgttgaa cgtgttacca
    33001 ccggaggctt cgacatcggc acgagcgtta ccaaacagcc cctgcaaata gcgcggcaca
    33061 gtatcaaagc caaaattagt tttaccgtca cggctggcat tcgcagagga gcttttgctg
    33121 gcgctgacgt tctcctgcaa cacgatggtc agcgtatcgc caatattgcg tggtcgacga
    33181 tcttcaaaca gcggttgata gccatagtta atcggctgag cagactggaa aatagaaccg
    33241 ttggcgacgg gcgtcggccc gggaaccggt tgtgcactgg tcgccccctg caccagcggc
    33301 gtggagggta tccaggcgca gccggttagt gaaagcacca acaagctgga aatggcataa
    33361 gtatgcgcag cgtttttttg catggctatc atcttcaaaa atcagtaaac cggtgaacct
    33421 gccaccggtt aagccttaga gttgcgtcag tttttgcagc atctgatcgg tggtggacac
    33481 cgctttactg ttgatttcgt aagcgcgttg cacctgaatc atattgacca gttcttccgc
    33541 cacgttgacg ttagacgttt caacataccc ttgatacagc agtcccgcgc cgttcaggcc
    33601 cggcgtgctt tcgttcggtg caccagagga ttgcgtttcg gtgtagaggt tttcgccaat
    33661 gctctccagc ccggtgtcat tcataaaggt ggtgagattg agctgcccaa cctgaaccgg
    33721 agctgcctgg ccttgttggg ttacgctgac cacgccatca cgaccgatgg tgatacttaa
    33781 cgcattcgcc ggaatggtga tcgctggctg cacctgaaaa ccaccagccg tcaccagctg
    33841 cccgttctga tccacctgga aagagccgtc acgggtatag gctgatgaac catctggcaa
    33901 catcacctgg aaaaagccct gccctttaat cgcgacatct ttgctgttgt tggtctgcga
    33961 caggtttccc tggctgtgta agcgttcagt ggcgaccggg cgtacccccg tgccgatttg
    34021 taatccggag ggtaaggtgg tttgttcgga agactgtgcc cccggctggc gaatggtttg
    34081 ataaagcaga tcttcaaaca ccgcgcgctg acgcttaaaa ccgttagtac tgacgtttgc
    34141 caggttgttg gcaatgacgt ccatattggt ttgctgggcg tcaaggcccg ttttggcgat
    34201 ccataatgaa ctgatcataa aatgtcctgt agtggattag tgacaagcag agcagaagca
    34261 cgcgacagtg tgaatttccc ctcaccctaa ccctctcccc aaaggggcga gggaatagtc
    34321 cgcgcgcttt tattgcaatg tcatcaatcg ttcaattaac tcatcgacag cagttggttg
    34381 gcacggcctg cgttatcatc gacgctgctg atcaccttca tctgcatttc aaaacgccgc
    34441 gcgctggcaa tcatgtcgct cattgccgca acggcattga cgttactgcc ttccagaacc
    34501 cccgacatca cacgcaaggt tggatctgcc tgcagtatcg gcccacgcgt ggcctggctt
    34561 tctgcgctta aacgaaaaat gccgtcgtca ccgcgttgca cttcgctgcc cgtggctttc
    34621 accagtttca gacgccccac tggcgcaacc gtatttgccg gatcgcccgg attgagcgcc
    34681 gagattgtgc catcggcagc aatagtgatt tccgcccctt ccggcacagc aattggccca
    34741 gcctcgccta tcaccggatg cccctgaatt gtcagttgcc cggtgggatc aacctgaatg
    34801 ctgccattac gcgtataccc ttcgctgccg tcagcggact gcacggccag ccagccatcc
    34861 tgctgcaacg caacgtccag cgggcgcgag gtgtaatcca tcttgccggg cgtcatgtct
    34921 gcacccggtg ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc
    34981 actgcacgca aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta
    35041 ctggcggtta ccgcctgttg attcagcgtc tggctggctg ctcccatcgc ggtataaatt
    35101 gcgtgatcca ttgagctatc ccgtcagcga ttagcgtaag ttaaccagcg tgttgaggat
    35161 ctggtcctgg gttttgatgg tctgggcgtt agactgatag ttacgctggg caacgatcat
    35221 attgaccagt tctttactga gatcgacgtt ggacgcttcc aacgcaccgt tggtcagggt
    35281 gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga
    35341 ccagacgttg tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat
    35401 ctgccccagc agttgtgttt gttcgttgga atagttgccg acaaccgtac cgtcatcatt
    35461 gatttgataa ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatatt
    35521 gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg tggcgggatc
    35581 tgcgccgtta attgcgccag tggtaatatt ctctgttgga tttgaggtca gaacgccatt
    35641 ggcattaaac accagcttca ttgcaggctc ggctgtacct gttggatcac tgctatcctg
    35701 ggtgtagacc tgccagttat tatccccggt cttcacaaag tagacgctca tgtcatgagc
    35761 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    35821 ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga
    35881 tgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt
    35941 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    36001 ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa
    36061 caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    36121 acctcgcccg gtgttggtgg tcgtgccatc ggtaaagtcc tgagtgatac cggcaacttt
    36181 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    36241 aaagccgtag gtggcggagt tggcgatatt gttgccaata acatcgaggt tggtggcggc
    36301 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    36361 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    36421 attaccgcgg ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac
    36481 cagttgtgta ccaccgttac tcgcgctaat cgctacattg taagaaccgt tcggcgcagt
    36541 gctgccgtca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg tcagttcacc
    36601 aatatcaatg gtgcgcacaa ccgcaccatt tttatcggtg atggtggccg taactttgtc
    36661 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagctc cttcttcact
    36721 gccggttccc gcaagaacag tggtgccggg gatcatcacg ccgtgaccga tcaggttact
    36781 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagtg tggtattgag
    36841 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    36901 cattggattg gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct
    36961 ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    37021 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    37081 gtaagggttt tcagcatcat acttttcacc gtgttgagca cttcaacatt ggcctgatag
    37141 ctgcgtgacg ccgacatggt gttaaccatc tccccgacga catcaacgtt cggcattttt
    37201 acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt gtccggggcc
    37261 tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    37321 acctggaata ccacctgttt tgcccgatat ggctgtccat cgggaccggt cacgctatca
    37381 gcatttgcca gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac
    37441 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    37501 atcatgcctt tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta
    37561 tcggcaaact gggtgcgttc gcgatccata tcgacggtat taccgtcaag cgaaggctgg
    37621 tccggaatac ggtattgcag ttctgcggta ggaggcgtca gcgcctgcgc cgggatgtgt
    37681 tgcgttgagg tcatcgtcag tgcaaccaca ctggttgcat cccgtccacg ttgcatgact
    37741 tttttaagtt cactggcaaa atcgatatcg cgcgcctgat aaccaggggt atcggcattg
    37801 gcgatgtttg ctgccagcac ttcctgacgc tgggcgcgca gattgagcgc ctcttgttga
    37861 aaacgtaagg cggcgtcgag cttatcgagc atatctcctc cgcaggtatc aaaattctgc
    37921 catcagctta aatgccttta cgcgcgcgtt atcggcggaa taaacgcaaa atgggtcgct
    37981 atttatgccg ttgatggtca ttgcggacag gtacaattca cgttgtagaa atggctgggg
    38041 gcgaaaatgc tgacaataaa acgtagcgtg gcgatcatcg cgatactgtt cagtccgtta
    38101 agtacggcga gcaatctcac atcgcaattg cacaactttt ttagcgccca actcgcgggg
    38161 gtaagtgatg aggttcgtgt ttctattcgt acagcgccca atctactacc gccatgcgag
    38221 cagccattgc tttcgatgag caataattcc cgcctgtggg gcaatgtgaa tgtgttggca
    38281 cgctgcggta acgacaaacg atatttacag gttaatgtgc aggccacagg aaattatgtg
    38341 gttgccgcga tgcccattgt gcggggagga aagctggaag ctggcaatgt caaactaaaa
    38401 cgcggacggc tggataccct gccaccgcgt acggtgctgg atatcaatca acttgttgat
    38461 gccattagcc tgcgcgatct atcacccgat caacctatcc agttaaccca gtttcgccag
    38521 gcatggcggg taaaagcggg acaacgcgtc aatgtgatcg ccagcggtga tgggtttagc
    38581 gccaacgcag aaggtcaggc gctgaacaat gcagccgtcg cacagaatgc gcgggtgcgc
    38641 atggtatcgg gacaggtagt cagcggcgtt gttgatgcag atgggaatat tcttataaac
    38701 ctgtaagctg ttaaagatta cccgtccctt gccgataaat aagcaacaca tgataaaagc
    38761 gccctcaatg aggaataaac catgagtatt gatcgcactt cgcctctgaa gcctgtaagc
    38821 accgttcaac cgcgcgaaac cactgacgcg ccggtaacga acagccgggc ggcaaaaaca
    38881 accgcctcca ccagcaccag tgtgacgtta agcgacgcgc aagcaaaact gatgcaaccc
    38941 ggcagcagtg atatcaatct tgaacgtgtc gaagcgttaa aactggcgat tcgtaacggt
    39001 gaactaaaaa tggacaccgg caaaattgcc gatgcgctga tcaacgaagc gcagcaagac
    39061 ttgcagagta actgatcgta tgacacgtct tgcagagatc ctcgaccaga tgtccgctgt
    39121 gcttaacgat ctcaaaacgg taatggatca agagcagcaa catctctcta tggggcagat
    39181 caacggcagc cagttgcaat ggattacaga acaaaaaagc tcactgctgg cgacgctgga
    39241 ttacctcgaa cagttacgca agaaagaacc caatacagca aatagcgttg atattagtca
    39301 acgctggcag gaaattactg tgaaaacgca gcaactacgc caaatgaatc aacataacgg
    39361 ctggttactg gaaggacaga ttgagcgcaa tcaacaggcg ctggaaatgt tgaaaccgca
    39421 tcaggaaccg acgctatatg gggcgaacgc tcagacctca acaacccatc gcggcggtaa
    39481 aaagatttcg atctgaatat tatctccggc ctgcactgca ggccggaatg cattgttaca
    39541 ccgtccggcg ggcaaattct ttaactttga agcccagtac cgccagtgca gcgaagtacg
    39601 cggcaatccc cgccagcacg accgccatta aacgcagtaa gcgccagggc atggtaccca
    39661 atgaccactc cggcatgata tgtaacatac ctaaaagcac gccagacatc accagtaccg
    39721 ccaccaccag acgcaacaga aacgccatcc agccgggttg cggagtaaag attttctgct
    39781 tacgcaactg ccagtaaagc agcgaagcat tcagacacgc cgccagacca atagaaagtg
    39841 acagcccggc atgtttcaac ggaccaataa acgccaggtt catcaattgc gttaaaatca
    39901 gcgtaacgat ggcaattttc actggcgttt taatgtcctg gcgggaataa aagccaggag
    39961 ccaacacttt cactacaatc aggccaatca aacccaccga gtaggcaatt aacgcccgct
    40021 gggtcatcag cgcatcaaac gcggtaaatt taccgtactg gaacagcgaa acggttaacg
    40081 gacctgaaag aatgcccaac gcaaccgcac tcggcagcgc caacaggaaa caaagacgca
    40141 atccccagtc catcaaacgg ttgtattcat catgattgcc actggcaaaa cttttcgaca
    40201 gcgacggcag caaaatggtg ccaagcgcca cgcccagcac accggacgga aactccatta
    40261 agcggtcggc gtaatacatc caagacaccg aaccggaagc aagaaacgag gcaaaaatgg
    40321 tgttgatgat taaggagatc tggctcacag agacgccaag gatcgccggt cccatctgtt
    40381 tcaccacgcg cattgctccg gcatcgtgga agttaatgcg cggcaggacc agcatgccga
    40441 tcttcttcag gtgcggtagc tgatacacca gctgcaggat gccgccgacc gtaacagccc
    40501 acgccagcgc cagcaccggt gggttaaagt acggtgcggc aaacagcgcg aaaccaatca
    40561 tgctgatgtt aagcagtgtt ggagcaaacg ccggaatcga gaagcggttc cacgtattca
    40621 gaatcgctcc caccagcgac gccagggaga tcagcaagat atagggaaag gtaatcttta
    40681 gtagctggct ggtcagggca aatttgtcag ctgtgtcagc gaagcctggc gcggtcacca
    40741 tgatcaccca cggtgcggcg agcatgccag cgaccgtcac aaccgccagc gcgagtgtca
    40801 gcaggccaga aacataagag acaaagaccc gcgtggcgtc ttcaccctgc ttacttttat
    40861 attccgccag aatcggtaca aatgcctggg aaaatgcccc ttcggcaaag atacggcgta
    40921 acaagttagg aagtttaaaa gcgacgaaaa aggcgtcggt tgccatccct gcgccaaaga
    40981 ttctggcgac aattgcgtct cgtgcgaagc caagcacacg cgaaaacatg gtcatcgagc
    41041 tgacggcggc cagcgatttt aataaattca tcggtgttct aatccagacc cacgggcaaa
    41101 acgcctgcaa ggcaggcgct gaaagaggcg aatagtctac ctggattatg gtgaattgct
    41161 accgccagat gttacagggt ttattcactc atcgcatcgc gccagatctt gtcaacgata
    41221 cgttgcgcca gcacggcctg ttcgccggcg gtttgcggaa ctgtctgatt ttgcacacat
    41281 tcaatgaagt gccgcgcaca gccgacaaat ccacgttgtt caagagtgct ctgccaacca
    41341 ggaatcggtt tatgcactac gccctgcccg cgctcctcac gccattcgcg catatccgta
    41401 atgtcgatga gcgcaccgtc agtcacggcc tgcacggttt cacgctgact tccggcacgg
    41461 cgatgcatac aggtggtgat ctgcaaagga ccagccgaaa aatggtgctc ggcaaacagc
    41521 atttcgccag cgtcgttagt cagtagcgta ccgccatcca gagaggcttt gccacccgac
    41581 aaccacagcg cggtatccac cacatgcaga taatcatcca gcaacgtgaa ataaagatcg
    41641 tgtggcccga cgctattgct acgatgttta tccattctta acgaggctgc ggtggcgagt
    41701 tgcgttttta actctccgta gagtggtgcg aaacgacggt taaaaccgac catcaacgtc
    41761 agttttttcc gcgccgccag ttccaccagc cgttcagcat cgcgcagatt ttctgccagc
    41821 ggtttatcga cacagacatg tacccccgca ttgagtaacg tactgaccac gtcaaagtgg
    41881 ctggcggtgc tggaatgcac aaaaaccgca tcgcaactgg cggcaaggct ggataacgaa
    41941 tcggcataag gaatgcgcca gctttcacaa attggcaggg ctttcgcgcg cgtaggcgac
    42001 caggctcctt gtaacatcca gtcagacgct gccgccagca ccggtaacca cgctttttgc
    42061 gcaatgccgc ctaatcccac aacgccgata cgtaattttt tcactttaat ctcccagatg
    42121 ggccagcaac gaatcaagac gctgtttcag ttctgccact tcgatttcca gggcttcgac
    42181 gcgggcctgt aaatcaccat caaccgcgtt cgacatatcc gttaccgccg gctgatcctc
    42241 tacctcacca ctgaaaagat gcatgtagcg gctttcgcgt ttacccggtt cgcgggccag
    42301 acgcaccaca aaaggaccat cttcgcgatt tgccagttgt tccagcgtcg actccacttc
    42361 cgccatatcg ctgaattcat acattcgcgc ggcgcggctg cgcagttcac ctggcgtctg
    42421 ggcaccacgc aataacaacg tggtgattaa cgccacttcc gctgcgctca gtttcagatc
    42481 gccaaattct gaattacaaa aacgttgctc atatttggtg acccgattac caaaaccgct
    42541 cactgtgcgt agataatgac gtttgaccag attatccagc tgttcctgca cttcggattc
    42601 actcagattc atgaccggtt cacggttcgt tttctgatta caggccgtga ctacaccatt
    42661 gactgagagc gggtattgct ccggcgtggt cacctgcttt tccagcaagc aaccaatcac
    42721 gcgagcttca agggcagtaa gttgatattt catgagattc tccttagcgg ccgggcgtcc
    42781 agtctggggt agttaatgcc gtcagtacgt gatcgcgcca ttgtccatca atcaacagat
    42841 agtctttcgc atagccttct ttttcaaaac ccagtcgcgc cagtaaatca ccgctgcgtt
    42901 tattgtgcgg catataatta gccataatgc gatgaatatg ttgggttcgc tgcatataac
    42961 gaatggctgc ggtcagggct tcaaacatga gtcctttgcc ctgccatttt tgcccaatcg
    43021 aataaccgag atagcaggca tgaaaagagc cacgaacaac attggaaaaa ttggcaacgc
    43081 caataatctc tttttcgtcc gggtcgaata agccaaagta gaaagctgaa ccttgtttat
    43141 gaaattcgtt aatcatcccc agcctggcct gccagcctga tggataacag tggctttcgt
    43201 cgcgcactgg ctcccagggc ttgaggaaat ggcgattctc tgcgtaataa tccgcaagac
    43261 gccaggcatc acgatcatgc accagacgca cgaccagtcg gtctgtggtt aagcgcactt
    43321 ttggcacgtt actgcgatag ccaaacattc tatacctact ccttcccgta acgtctttaa
    43381 tacgctgtaa gtattactat acctgcgcaa ccagaccctg tgaaaagtcc aaaatgccat
    43441 tttctgcatt attcacatat ttgataaaaa ctctctatca aagcgacgtt ttgataaaaa
    43501 aatattgtcc ccgaagtgat gggaaaagtc ttagcggcaa tgcagaatag aaagtgctca
    43561 tcttttattt cccgggaggg gaaatgtccc gcgtatcgca ggcgaggaac ctgggtaaat
    43621 atttcctgct catcgataat atgctggtcg tgctggggtt ctttgttgtc ttcccgctga
    43681 tctctatccg cttcgttgat caaatgggct gggccgccgt catggtcggt attgctctcg
    43741 gtctacgcca gtttattcag caaggtctgg gtattttcgg cggcgcaatt gccgaccgct
    43801 ttggtgccaa accgatgatt gttaccggta tgctgatgcg cgccgccgga ttcgccacaa
    43861 tgggtatcgc ccacgaaccg tggctattgt ggttttcatg cctgctctcg ggactcggcg
    43921 gcacgttgtt tgatccgccg cgttcggcgc tggtggtgaa actaatccgt ccacaacaac
    43981 gtggtcgttt tttctcgctg ttgatgatgc aggacagtgc cggtgcggtc attggcgcat
    44041 tgttggggag ctggctgttg caatacgact ttcgcctggt ctgcgccaca ggggccgttt
    44101 tgtttgtgct gtgtgcggcg ttcaatgcgt ggttgttacc ggcatggaaa ctctccaccg
    44161 tacacacgcc cgttcgcgaa ggcatgaccc gcgtgatgcg tgacaagcgt tttgtcacct
    44221 atgtcctgac gctggcgggt tactacatgc tggctgtaca agtgatgctg atgctgccaa
    44281 ttatggtcaa cgacgtggct ggcgcaccct ctgccgttaa atggatgtat gccattgaag
    44341 cgtgtctgtc gttaacgttg ctctacccta tcgcccgctg gagtgaaaag cattttcgtc
    44401 tggaacaccg gttgatggct gggctgttga taatgtcatt aagcatgatg ccggtgggca
    44461 tggtcagcgg cctgcaacaa cttttcaccc tgatttgtct gttttatatc gggtcgatca
    44521 ttgccgagcc tgcgcgtgaa accttaagtg cttcgctggc ggacgcaaga gctcgcggca
    44581 gctatatggg gtttagccgt ctgggtctgg cgattggcgg cgctattggt tatatcggtg
    44641 gtggctggct gtttgacctg ggcaaatcgg cgcaccagcc agagcttccg tggatgatgc
    44701 tgggaattat tggcatcttc actttccttg cgctgggttg gcagtttagt cagaaacgcg
    44761 ccgcgcgtcg tttgctggaa cgcgacgcct gatttgatac tcccccctga ctcctccata
    44821 ctgaaagtga actgacagcc aaatggagga gtcacgtgaa gctatacatt tacgatcact
    44881 gcccttactg cctcaaagcc cgcatgattt tcggcctgaa aaatatcccc gtcgaattac
    44941 atgttctgct caacgacgac gcagaaacac ccacccggat ggtcggtcaa aaacaggttc
    45001 ccattctgca aaaagatgac agccgctata tgccagaaag catggatatc gttcactatg
    45061 tcgataaact cgacggcaaa ccgttactga ccggcaaacg ttcccctgcc attgaagagt
    45121 ggctgcgcaa ggtcaatggc tacgcaaaca aactgctgtt gccgcgtttt gccaaatcgg
    45181 catttgatga gttttctact cccgccgcgc gcaaatattt cgtcgacaag aaagaggcca
    45241 gcgcgggtaa ttttgccgac ctgctggccc actctgacgg tctgattaag aatatcagcg
    45301 atgatttgcg tgcgctggac aaactgatcg tcaaaccgaa cgccgtgaat ggcgaacttt
    45361 cggaagatga tattcagcta tttccgctac tgcgtaatct gacgctggta gccggaatca
    45421 actggccaag ccgcgttgct gattaccgcg ataatatggc gaaacagaca caaatcaatt
    45481 tgttatcatc aatggcgatt taatcctgac ccgccgcggc agcctgtatt cgcgggttgc
    45541 cgccgttttc agttttttct gctggcgagt ttatcgctgg tgacacatgc tgcgttgtgt
    45601 caggatattg acgcagattt catgtcgagg actaccatga acaagttttt atttgccgct
    45661 gcattgatcg tcagtggcct gctcgttggc tgtaatcaac tcacccaata caccatcacc
    45721 gaacaagaaa ttaaccagtc gcttgcgaaa cataataatt tctcaaaaga tatcggttta
    45781 cccggcgtgg ctgacgccca tattgttctg acaaacttga ccagccaaat tggtcgcgaa
    45841 gagccgaata aggttaccct aaccggagac gccaatctgg acatgaactc cctgttcggt
    45901 agtcagaaag cgaccatgaa actgaagctg aaagcgctgc ccgtgtttga taaagaaaaa
    45961 ggtgcgatct tcctgaaaga gatggaggtg gtcgatgcga cggtacaacc ggaaaaaatg
    46021 caaacggtga tgcaaacgtt gcttccctat ttgaaccagg cattacgcaa ttactttaac
    46081 cagcaacctg cttacgtcct gcgcgaagat ggcagtcagg gcgaagcaat ggcgaaaaaa
    46141 ctggcgaaag gcattgaagt gaagccaggc gaaattgtca ttccgtttac ggattaatga
    46201 cgagggcgca ttcgcgccct ttatttttcg tgcaaaggaa aacgtttccg cttatccttt
    46261 gtgtccggca aaaacatccc ttcagccgga gcatagagat taatgactgc accatcccag
    46321 gtattaaaga tccgccgccc agacgactgg caccttcacc tccgcgatgg cgacatgtta
    46381 aaaactgtcg tgccgtatac cagcgaaatt tatggacggg ctatcgtaat gcccaatctg
    46441 gctccgcccg tgaccacagt tgaggctgcc gtggcgtatc gccaacgtat tcttgatgcc
    46501 gtacctgccg ggcacaattt caccccattg atgacctgtt atttaacaga ttcgctggat
    46561 cctaatgagc tggagcgcgg atttaacgaa ggcgtgttca ccgctgcaaa actttatccg
    46621 gcaaacgcaa ccactaactc cagccacggc gtgacgtcaa ttgacgcaat catgccggta
    46681 cttgagcgca tggaaaaaat cggtatgccg ctactggtgc atggtgaagt gacacatgca
    46741 gatatcgaca tttttgatcg tgaagcgcgc tttatagaaa gcgtgatgga acctctgcgc
    46801 cagcgcctga ctgcgctgaa agtcgttttt gagcacatca ccaccaaaga tgctgccgac
    46861 tatgtccgtg acggaaatga acggctggct gccaccatca ctccgcagca tctgatgttt
    46921 aaccgcaacc atatgctggt tggtggcgtg cgtccgcacc tgtattgttt acccatcctc
    46981 aaacgcaata ttcaccaaca ggcattgcgt gaactggtcg ccagcggttt taatcgagta
    47041 ttcctcggta cggattctgc gccacatgca cgtcatcgca aagagagcag ttgcggctgc
    47101 gcgggctgct tcaacgcccc aaccgcgctg ggcagttacg ctaccgtctt tgaagagatg
    47161 aatgctctgc agcactttga agcattctgt tctgtaaacg gcccgcagtt ctatggcttg
    47221 ccggtcaacg acacattcat cgaactggta cgtgaagagc aacaggttgc tgaaagcatc
    47281 gcactgactg atgacaccct ggtgccattc ctcgccgggg aaacggtacg ctggtccgtt
    47341 aaacaataaa aatcgtagct tcctgttgtc attaggttat tttacctgta taaataacca
    47401 gtatattcaa cagggggcta ttatgcgaat tgaagtcacc atagcgaaaa cttctccatt
    47461 gccagctggg gctattgacg ccctggctgg cgaactttcc cgccgtattc agtatgcgtt
    47521 tcctgataat gaaggccacg tatcggtacg ttatgccgca gcgaataatt tatcggttat
    47581 tggcgcgaca aaagaagata aacagcgcat tagcgaaatt ctccaggaaa cgtgggaaag
    47641 cgccgatgac tggtttgtca gcgaataata tgcagtgatt tttgtttttg ccgggtcgcc
    47701 ccggcttttt tattttactt acgaattttg agtaca
//
LOCUS       DAZFVO020000016        47617 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFVO020000016 DAZFVO020000000
VERSION     DAZFVO020000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.016"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            60..578
                     /gene="yfiR"
                     /locus_tag="ACHKNB_RS04190"
                     /old_locus_tag="pgaptmp_000838"
     CDS             60..578
                     /gene="yfiR"
                     /locus_tag="ACHKNB_RS04190"
                     /old_locus_tag="pgaptmp_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HGU4194405.1"
                     /translation="MRFSHRLFLLLILLLTRAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            568..1794
                     /gene="dgcN"
                     /locus_tag="ACHKNB_RS04195"
                     /old_locus_tag="pgaptmp_000839"
     CDS             568..1794
                     /gene="dgcN"
                     /locus_tag="ACHKNB_RS04195"
                     /old_locus_tag="pgaptmp_000839"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HGU4194406.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQDIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEISKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1810..2292
                     /gene="yfiB"
                     /locus_tag="ACHKNB_RS04200"
                     /old_locus_tag="pgaptmp_000840"
     CDS             1810..2292
                     /gene="yfiB"
                     /locus_tag="ACHKNB_RS04200"
                     /old_locus_tag="pgaptmp_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4194407.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2369..2716)
                     /gene="rplS"
                     /locus_tag="ACHKNB_RS04205"
                     /old_locus_tag="pgaptmp_000841"
     CDS             complement(2369..2716)
                     /gene="rplS"
                     /locus_tag="ACHKNB_RS04205"
                     /old_locus_tag="pgaptmp_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGU4194408.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2758..3525)
                     /gene="trmD"
                     /locus_tag="ACHKNB_RS04210"
                     /old_locus_tag="pgaptmp_000842"
     CDS             complement(2758..3525)
                     /gene="trmD"
                     /locus_tag="ACHKNB_RS04210"
                     /old_locus_tag="pgaptmp_000842"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGU4194409.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3556..4104)
                     /gene="rimM"
                     /locus_tag="ACHKNB_RS04215"
                     /old_locus_tag="pgaptmp_000843"
     CDS             complement(3556..4104)
                     /gene="rimM"
                     /locus_tag="ACHKNB_RS04215"
                     /old_locus_tag="pgaptmp_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGU4194410.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4123..4371)
                     /gene="rpsP"
                     /locus_tag="ACHKNB_RS04220"
                     /old_locus_tag="pgaptmp_000844"
     CDS             complement(4123..4371)
                     /gene="rpsP"
                     /locus_tag="ACHKNB_RS04220"
                     /old_locus_tag="pgaptmp_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGU4194411.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4620..5981)
                     /gene="ffh"
                     /locus_tag="ACHKNB_RS04225"
                     /old_locus_tag="pgaptmp_000845"
     CDS             complement(4620..5981)
                     /gene="ffh"
                     /locus_tag="ACHKNB_RS04225"
                     /old_locus_tag="pgaptmp_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGU4194412.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6148..6939
                     /gene="ypjD"
                     /locus_tag="ACHKNB_RS04230"
                     /old_locus_tag="pgaptmp_000846"
     CDS             6148..6939
                     /gene="ypjD"
                     /locus_tag="ACHKNB_RS04230"
                     /old_locus_tag="pgaptmp_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HGU4194413.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6960..8246
                     /gene="yfjD"
                     /locus_tag="ACHKNB_RS04235"
                     /old_locus_tag="pgaptmp_000847"
     CDS             6960..8246
                     /gene="yfjD"
                     /locus_tag="ACHKNB_RS04235"
                     /old_locus_tag="pgaptmp_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HGU4194414.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8301..8894)
                     /gene="grpE"
                     /locus_tag="ACHKNB_RS04240"
                     /old_locus_tag="pgaptmp_000848"
     CDS             complement(8301..8894)
                     /gene="grpE"
                     /locus_tag="ACHKNB_RS04240"
                     /old_locus_tag="pgaptmp_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGU4194415.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9017..9895
                     /gene="nadK"
                     /locus_tag="ACHKNB_RS04245"
                     /old_locus_tag="pgaptmp_000849"
     CDS             9017..9895
                     /gene="nadK"
                     /locus_tag="ACHKNB_RS04245"
                     /old_locus_tag="pgaptmp_000849"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HGU4194416.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            9981..11642
                     /gene="recN"
                     /locus_tag="ACHKNB_RS04250"
                     /old_locus_tag="pgaptmp_000850"
     CDS             9981..11642
                     /gene="recN"
                     /locus_tag="ACHKNB_RS04250"
                     /old_locus_tag="pgaptmp_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGU4194417.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11791..12132
                     /gene="bamE"
                     /locus_tag="ACHKNB_RS04255"
                     /old_locus_tag="pgaptmp_000851"
     CDS             11791..12132
                     /gene="bamE"
                     /locus_tag="ACHKNB_RS04255"
                     /old_locus_tag="pgaptmp_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HGU4194418.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12194..12484)
                     /gene="yfjF"
                     /locus_tag="ACHKNB_RS04260"
                     /old_locus_tag="pgaptmp_000852"
     CDS             complement(12194..12484)
                     /gene="yfjF"
                     /locus_tag="ACHKNB_RS04260"
                     /old_locus_tag="pgaptmp_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HGU4194419.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12474..12911)
                     /gene="ratA"
                     /locus_tag="ACHKNB_RS04265"
                     /old_locus_tag="pgaptmp_000853"
     CDS             complement(12474..12911)
                     /gene="ratA"
                     /locus_tag="ACHKNB_RS04265"
                     /old_locus_tag="pgaptmp_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HGU4194420.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13082..13564
                     /gene="smpB"
                     /locus_tag="ACHKNB_RS04270"
                     /old_locus_tag="pgaptmp_000854"
     CDS             13082..13564
                     /gene="smpB"
                     /locus_tag="ACHKNB_RS04270"
                     /old_locus_tag="pgaptmp_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGU4194421.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13779..14141
                     /gene="ssrA"
                     /locus_tag="ACHKNB_RS04275"
                     /old_locus_tag="pgaptmp_000855"
     tmRNA           13779..14141
                     /gene="ssrA"
                     /locus_tag="ACHKNB_RS04275"
                     /old_locus_tag="pgaptmp_000855"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14265..14747
                     /locus_tag="ACHKNB_RS04280"
                     /old_locus_tag="pgaptmp_000856"
     CDS             14265..14747
                     /locus_tag="ACHKNB_RS04280"
                     /old_locus_tag="pgaptmp_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194422.1"
                     /translation="MEELHFVYINANGRIGVHSIQSISYSENHIQGICKNTDRIKTFR
                     KDRILKQYDSPEQAIQECASFLPENYSHLTKQSGPKKNTFDVCFTGFKKADKERLVDK
                     ANEQGLTVRTSVTQSLQMLCCGYNAGPSKVSAARMKGTIIIDEPGFIHFLETGEIPDE
                     "
     gene            complement(14774..14992)
                     /locus_tag="ACHKNB_RS04285"
                     /old_locus_tag="pgaptmp_000857"
     CDS             complement(14774..14992)
                     /locus_tag="ACHKNB_RS04285"
                     /old_locus_tag="pgaptmp_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4194423.1"
                     /translation="MMNCPKCGHAAHTRSSFQVTESTKERYCQCQNINCGSTFVTHET
                     VVRFIVTPALIATAPPHPLPGGQGHMNF"
     gene            complement(15061..16161)
                     /locus_tag="ACHKNB_RS04290"
                     /old_locus_tag="pgaptmp_000858"
     CDS             complement(15061..16161)
                     /locus_tag="ACHKNB_RS04290"
                     /old_locus_tag="pgaptmp_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HGU4194424.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDKRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHNTTIGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSIELEVKIDDLEME"
     gene            complement(16158..16643)
                     /locus_tag="ACHKNB_RS04295"
                     /old_locus_tag="pgaptmp_000859"
     CDS             complement(16158..16643)
                     /locus_tag="ACHKNB_RS04295"
                     /old_locus_tag="pgaptmp_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4194425.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            complement(16640..19717)
                     /locus_tag="ACHKNB_RS04300"
                     /old_locus_tag="pgaptmp_000860"
     CDS             complement(16640..19717)
                     /locus_tag="ACHKNB_RS04300"
                     /old_locus_tag="pgaptmp_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4194426.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIQNTRNSLK
                     ELNKQAGRIDEFRKTRSQLAITANNLNAAREEAAKLATQFAATNRPTAAQAKLFSQAK
                     TRVQELQQTYNGLLGAVQRQRQALKESGIDTRQLSSAQRELKKNAEETRQALEGQKKA
                     LKRLGEQQARMNAAREQYSRRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSITGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVVLAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLASAGTVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PERVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     HRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            complement(19710..19829)
                     /locus_tag="ACHKNB_RS04305"
                     /old_locus_tag="pgaptmp_000861"
     CDS             complement(19710..19829)
                     /locus_tag="ACHKNB_RS04305"
                     /old_locus_tag="pgaptmp_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HGU4194427.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            complement(19844..20146)
                     /locus_tag="ACHKNB_RS04310"
                     /old_locus_tag="pgaptmp_000862"
     CDS             complement(19844..20146)
                     /locus_tag="ACHKNB_RS04310"
                     /old_locus_tag="pgaptmp_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4194428.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(20202..20714)
                     /locus_tag="ACHKNB_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /pseudo
     CDS             complement(20202..20714)
                     /locus_tag="ACHKNB_RS04315"
                     /old_locus_tag="pgaptmp_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(20724..21896)
                     /locus_tag="ACHKNB_RS04320"
                     /old_locus_tag="pgaptmp_000864"
     CDS             complement(20724..21896)
                     /locus_tag="ACHKNB_RS04320"
                     /old_locus_tag="pgaptmp_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HGU4194429.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLTDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            complement(22039..22605)
                     /locus_tag="ACHKNB_RS04325"
                     /old_locus_tag="pgaptmp_000865"
     CDS             complement(22039..22605)
                     /locus_tag="ACHKNB_RS04325"
                     /old_locus_tag="pgaptmp_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4194430.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTSEQWAQIGRLLEDGES
                     RQRIALIFDVGVSTIYRKFPANKNNKSP"
     gene            22636..23046
                     /locus_tag="ACHKNB_RS04330"
                     /old_locus_tag="pgaptmp_000866"
     CDS             22636..23046
                     /locus_tag="ACHKNB_RS04330"
                     /old_locus_tag="pgaptmp_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194431.1"
                     /translation="MPVSRYLCIFINVGLGEAAKRGVGTGENQIPDMASFSGVRDYYG
                     KQLLPGGLILQWLTIPSSAAAKAVTLNNGNYQLSGYKWPQSFGVLFAVFATKVSGSTN
                     EAYAISVNRHSTDVIVTWNARKADDVHILGIGKL"
     gene            23043..23462
                     /locus_tag="ACHKNB_RS04335"
                     /old_locus_tag="pgaptmp_000867"
     CDS             23043..23462
                     /locus_tag="ACHKNB_RS04335"
                     /old_locus_tag="pgaptmp_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HGU4194432.1"
                     /translation="MKMKWSPSVQGFFSENNSDIPDDAFDIEDALYYELMNGQSTGKI
                     IINNPDNYPVLTEYPAKTQEQEIAEAEGMKSILIEQANEYMNSKQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(23434..24036)
                     /gene="tfaE"
                     /locus_tag="ACHKNB_RS04340"
                     /old_locus_tag="pgaptmp_000868"
     CDS             complement(23434..24036)
                     /gene="tfaE"
                     /locus_tag="ACHKNB_RS04340"
                     /old_locus_tag="pgaptmp_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4194433.1"
                     /translation="MNKAVLNSELITTKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSADLNSWEYVPDHRGEVVYNTETGESKEITAPGNYPEN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVEAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(24036..25580)
                     /locus_tag="ACHKNB_RS04345"
                     /old_locus_tag="pgaptmp_000869"
     CDS             complement(24036..25580)
                     /locus_tag="ACHKNB_RS04345"
                     /old_locus_tag="pgaptmp_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4194434.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNWPESYKPQLAEGSGRTQTVRMVLITSSTDNIILKIDPAVVLATRKYVDDKVLELK
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEGSALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELA
                     KAYPTNKLPDLRGEFIRGWDDERGVDSGRTLLSAQGDAIRNITGGFGQLRVNSEINAI
                     VDVQSVSGAFYGGTSVRNNINVSMTYANDRKIRQDVHFSAANVVPTANENRPRNIAFN
                     YIVRAA"
     gene            complement(25577..26182)
                     /locus_tag="ACHKNB_RS04350"
                     /old_locus_tag="pgaptmp_000870"
     CDS             complement(25577..26182)
                     /locus_tag="ACHKNB_RS04350"
                     /old_locus_tag="pgaptmp_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HGU4194435.1"
                     /translation="MNSLLPPGSTPLERRLARTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVERWDESWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(26175..27083)
                     /locus_tag="ACHKNB_RS04355"
                     /old_locus_tag="pgaptmp_000871"
     CDS             complement(26175..27083)
                     /locus_tag="ACHKNB_RS04355"
                     /old_locus_tag="pgaptmp_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HGU4194436.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFEALLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVENALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(27070..27429)
                     /locus_tag="ACHKNB_RS04360"
                     /old_locus_tag="pgaptmp_000872"
     CDS             complement(27070..27429)
                     /locus_tag="ACHKNB_RS04360"
                     /old_locus_tag="pgaptmp_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HGU4194437.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(27426..28004)
                     /locus_tag="ACHKNB_RS04365"
                     /old_locus_tag="pgaptmp_000873"
     CDS             complement(27426..28004)
                     /locus_tag="ACHKNB_RS04365"
                     /old_locus_tag="pgaptmp_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4194438.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDREHWLCRVKTGDLETNWIN
                     WLTLRAGNARTWWKPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(28073..28519)
                     /locus_tag="ACHKNB_RS04370"
                     /old_locus_tag="pgaptmp_000874"
     CDS             complement(28073..28519)
                     /locus_tag="ACHKNB_RS04370"
                     /old_locus_tag="pgaptmp_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4194439.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASEDSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(28512..28943)
                     /locus_tag="ACHKNB_RS04375"
                     /old_locus_tag="pgaptmp_000875"
     CDS             complement(28512..28943)
                     /locus_tag="ACHKNB_RS04375"
                     /old_locus_tag="pgaptmp_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4194440.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            complement(28906..29154)
                     /gene="lysC"
                     /locus_tag="ACHKNB_RS04380"
                     /old_locus_tag="pgaptmp_000876"
     CDS             complement(28906..29154)
                     /gene="lysC"
                     /locus_tag="ACHKNB_RS04380"
                     /old_locus_tag="pgaptmp_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HGU4194441.1"
                     /translation="MLTLSGCGNAPHSPEVQLTVSGCPRVTPCRLDQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(29039..29467)
                     /gene="lysB"
                     /locus_tag="ACHKNB_RS04385"
                     /old_locus_tag="pgaptmp_000877"
     CDS             complement(29039..29467)
                     /gene="lysB"
                     /locus_tag="ACHKNB_RS04385"
                     /old_locus_tag="pgaptmp_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HGU4194442.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGSGSAAH"
     gene            complement(29464..29841)
                     /locus_tag="ACHKNB_RS04390"
                     /old_locus_tag="pgaptmp_000878"
     CDS             complement(29464..29841)
                     /locus_tag="ACHKNB_RS04390"
                     /old_locus_tag="pgaptmp_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HGU4194443.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALVALIKNGPLWQELRASLLKTITRKENVFTSWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(29843..30355)
                     /locus_tag="ACHKNB_RS04395"
                     /old_locus_tag="pgaptmp_000879"
     CDS             complement(29843..30355)
                     /locus_tag="ACHKNB_RS04395"
                     /old_locus_tag="pgaptmp_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4194444.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(30336..30551)
                     /locus_tag="ACHKNB_RS04400"
                     /old_locus_tag="pgaptmp_000880"
     CDS             complement(30336..30551)
                     /locus_tag="ACHKNB_RS04400"
                     /old_locus_tag="pgaptmp_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HGU4194445.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(30555..30758)
                     /locus_tag="ACHKNB_RS04405"
                     /old_locus_tag="pgaptmp_000881"
     CDS             complement(30555..30758)
                     /locus_tag="ACHKNB_RS04405"
                     /old_locus_tag="pgaptmp_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HGU4194446.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(30758..31222)
                     /locus_tag="ACHKNB_RS04410"
                     /old_locus_tag="pgaptmp_000882"
     CDS             complement(30758..31222)
                     /locus_tag="ACHKNB_RS04410"
                     /old_locus_tag="pgaptmp_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HGU4194447.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(31318..31968)
                     /locus_tag="ACHKNB_RS04415"
                     /old_locus_tag="pgaptmp_000883"
     CDS             complement(31318..31968)
                     /locus_tag="ACHKNB_RS04415"
                     /old_locus_tag="pgaptmp_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HGU4194448.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMSDAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(31972..33030)
                     /locus_tag="ACHKNB_RS04420"
                     /old_locus_tag="pgaptmp_000884"
     CDS             complement(31972..33030)
                     /locus_tag="ACHKNB_RS04420"
                     /old_locus_tag="pgaptmp_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HGU4194449.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            complement(33047..33880)
                     /locus_tag="ACHKNB_RS04425"
                     /old_locus_tag="pgaptmp_000885"
     CDS             complement(33047..33880)
                     /locus_tag="ACHKNB_RS04425"
                     /old_locus_tag="pgaptmp_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HGU4194450.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQEDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSKLETALQAQQAAFNELVDKLSHEDCRQDYRQRATGGNAPADTLTN
                     C"
     gene            34023..35789
                     /locus_tag="ACHKNB_RS04430"
                     /old_locus_tag="pgaptmp_000886"
     CDS             34023..35789
                     /locus_tag="ACHKNB_RS04430"
                     /old_locus_tag="pgaptmp_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HGU4194451.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEMEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            35789..36823
                     /locus_tag="ACHKNB_RS04435"
                     /old_locus_tag="pgaptmp_000887"
     CDS             35789..36823
                     /locus_tag="ACHKNB_RS04435"
                     /old_locus_tag="pgaptmp_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4194452.1"
                     /translation="MGKSKKNRAAATNQIQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASHVFVRNELIPLQKRLIELNEWLNDEII
                     RFDEYKLGNT"
     gene            complement(36861..37709)
                     /locus_tag="ACHKNB_RS04440"
                     /old_locus_tag="pgaptmp_000888"
     CDS             complement(36861..37709)
                     /locus_tag="ACHKNB_RS04440"
                     /old_locus_tag="pgaptmp_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194453.1"
                     /translation="MLVRGTSWRQGHVLKHDDAVSLGILQPDETNHKVVVITHDCDLQ
                     SNSEKNVELMFGPLKKGSSRMKRAKHPRILDLCFENPESAKKNAVELRHERKVIIPKE
                     AFCCEENDPAFSISTEEKQALKQWLAAKYGRPAFPNSFEERLRAYDEDKKFIFEKEVA
                     DIIATNAEHLIGVFFDLGEERFNDLEEGIPYELSINVVYDATEGGPDARRAAEQTCSD
                     LKALFYKFYGDPTLGHSELIELNTCIAVADTHFSLYALRRMDQWRVEYISLEEDSYGD
                     FIGAGV"
     gene            complement(37719..38381)
                     /locus_tag="ACHKNB_RS04445"
                     /old_locus_tag="pgaptmp_000889"
     CDS             complement(37719..38381)
                     /locus_tag="ACHKNB_RS04445"
                     /old_locus_tag="pgaptmp_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4194454.1"
                     /translation="MYAIPTGSIGSVDRYSPNLQSSSCVSGVVAVAASLLLVGTGASY
                     PVNAYKQWRQYVQPRVQFAFDSIESTYTPAISPEVDVRNVAQHLANIREVLSPSMSEL
                     AKDLGITRQALYKWLSGENQPDDASKVQFITNLSNVADSFSKAGLHDAKLLVKMKAFN
                     GKSLMDVVKEGEDWNRAVQVLIDEAKAMNAAAESANYLASKAKPTDDWKSSISIPGTV
                     EE"
     gene            complement(38401..39210)
                     /locus_tag="ACHKNB_RS04450"
                     /old_locus_tag="pgaptmp_000890"
     CDS             complement(38401..39210)
                     /locus_tag="ACHKNB_RS04450"
                     /old_locus_tag="pgaptmp_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HGU4194455.1"
                     /translation="MSTNSERMSNAPVYYALVQVKFTPIAAMSKYVPDIQDALRVEGF
                     PLFEVSNTTQLKFEIKNPNEPPVHSFEPVTSWLMIDADRRSGFVLGNDFITFHTTYYD
                     NHVSFISSLILGLSKVLEFAKPSLLSRIGLRYLDAVFPEKSETIEQYLVKELHGVDFG
                     WTPIQSIQESVYQTCVEPLIPNGFMVSRIHKMNGQLGFPPDMIPNGLLPLPRFSNTEH
                     RMHAIIDTDHYVEGNMSTDLQLIEKQILSLHSKVKEAFEGMVSDFARTKWH"
     gene            complement(39574..39807)
                     /locus_tag="ACHKNB_RS04455"
                     /old_locus_tag="pgaptmp_000891"
     CDS             complement(39574..39807)
                     /locus_tag="ACHKNB_RS04455"
                     /old_locus_tag="pgaptmp_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4194456.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(39818..40006)
                     /locus_tag="ACHKNB_RS04460"
                     /old_locus_tag="pgaptmp_000892"
     CDS             complement(39818..40006)
                     /locus_tag="ACHKNB_RS04460"
                     /old_locus_tag="pgaptmp_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194457.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYNRSMDVIS"
     gene            complement(40160..42574)
                     /locus_tag="ACHKNB_RS04465"
                     /old_locus_tag="pgaptmp_000893"
     CDS             complement(40160..42574)
                     /locus_tag="ACHKNB_RS04465"
                     /old_locus_tag="pgaptmp_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HGU4194458.1"
                     /translation="MSHADMNNCSGFNEVAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYATDNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPGMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSTSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWWEEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQQGKDKSADGDTRTDITRMDDKELHDYLHSMSKKERRELAARLHLVKPKRCK
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSISSGAGLRIFYRNQRLQEDD
                     KWRNLY"
     gene            complement(42571..43428)
                     /locus_tag="ACHKNB_RS04470"
                     /old_locus_tag="pgaptmp_000894"
     CDS             complement(42571..43428)
                     /locus_tag="ACHKNB_RS04470"
                     /old_locus_tag="pgaptmp_000894"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGU4194459.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVD
                     SSAVYGVRA"
     gene            complement(43425..43727)
                     /locus_tag="ACHKNB_RS04475"
                     /old_locus_tag="pgaptmp_000895"
     CDS             complement(43425..43727)
                     /locus_tag="ACHKNB_RS04475"
                     /old_locus_tag="pgaptmp_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HGU4194460.1"
                     /translation="MTGAVRIHQLKIAPKYFNAVVAGQKTAELRKDDRGYKVGDVLSL
                     CEWKHGVFTGREWAAVISHVLPVNDVMAVSEQWVMLSIRSLTPLEALGYVIAGGAV"
     gene            complement(43724..43951)
                     /locus_tag="ACHKNB_RS04480"
                     /old_locus_tag="pgaptmp_000896"
     CDS             complement(43724..43951)
                     /locus_tag="ACHKNB_RS04480"
                     /old_locus_tag="pgaptmp_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HGU4194461.1"
                     /translation="MADAMDLVQQRVEEERQSHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(43951..44184)
                     /locus_tag="ACHKNB_RS04485"
                     /old_locus_tag="pgaptmp_000897"
     CDS             complement(43951..44184)
                     /locus_tag="ACHKNB_RS04485"
                     /old_locus_tag="pgaptmp_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HGU4194462.1"
                     /translation="MRNIEILTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(44252..44593)
                     /locus_tag="ACHKNB_RS04490"
                     /old_locus_tag="pgaptmp_000898"
     CDS             complement(44252..44593)
                     /locus_tag="ACHKNB_RS04490"
                     /old_locus_tag="pgaptmp_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HGU4194463.1"
                     /translation="MAINGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPKRLTMPN"
     gene            complement(44711..45007)
                     /locus_tag="ACHKNB_RS04495"
                     /old_locus_tag="pgaptmp_000899"
     CDS             complement(44711..45007)
                     /locus_tag="ACHKNB_RS04495"
                     /old_locus_tag="pgaptmp_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HGU4194464.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCHSQDELL
                     SELTTRKRRKSKCMQRKVKWFISFVTEGRVIQYLKMICSVVIRRCRAMAVTFSR"
     gene            complement(45015..45524)
                     /locus_tag="ACHKNB_RS04500"
                     /old_locus_tag="pgaptmp_000900"
     CDS             complement(45015..45524)
                     /locus_tag="ACHKNB_RS04500"
                     /old_locus_tag="pgaptmp_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HGU4194465.1"
                     /translation="MFDYKISKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMVSAVDTVT
                     GLGASFGLL"
     gene            complement(45557..45799)
                     /locus_tag="ACHKNB_RS04505"
                     /old_locus_tag="pgaptmp_000901"
     CDS             complement(45557..45799)
                     /locus_tag="ACHKNB_RS04505"
                     /old_locus_tag="pgaptmp_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4194466.1"
                     /translation="MSTDISIRVPKEMATPAEFAEWEGISRGSVYQKIHHGQLAKYMV
                     KKEKNKGRVSLRYLMYKTDQVRESLGHSNFRVIVGK"
     gene            45921..46553
                     /locus_tag="ACHKNB_RS04510"
                     /old_locus_tag="pgaptmp_000902"
     CDS             45921..46553
                     /locus_tag="ACHKNB_RS04510"
                     /old_locus_tag="pgaptmp_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HGU4194467.1"
                     /translation="MLNIRMGSDTGGKAAIERLLEAYGFTTKQALSEHLNVSKSTMAN
                     RVLRDSFPADWIIQCALETGVSLLWLATGQGSMKEGYEPEKRSHNENKQAIKPLSKLI
                     TPSIPKGTLENGQLSIDEEIFLDHSILPADYEESMFLETPTDCYLIDKSVKQVSNGFW
                     LINIDGMIIIAKIMRIPGNKIVVNQDEASFECSADDVEVIGRAVKVIKSI"
     gene            46556..47572
                     /locus_tag="ACHKNB_RS04515"
                     /old_locus_tag="pgaptmp_000903"
     CDS             46556..47572
                     /locus_tag="ACHKNB_RS04515"
                     /old_locus_tag="pgaptmp_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4194468.1"
                     /translation="MTVRKQPNGKWLCECYPNGRNGKRVRKQFATKGEAIAFESFTME
                     EVNKKPWLGEKEDRRHLSELIEQWYSLYGQTLADPKRLIAKLRIICNGLGDPIASELT
                     AGDFTKYREARLKGEVRNEDGTFMSPVKPRTVNLEQRNLSSVFGTLKKLGHWSAPNPL
                     AGLPTFKIAESELAFLTPEEIKRLLDACADSQSSSLLLIAKICLATGARWSEAENLQS
                     HQVSKYRITYTKTKGKKNRTVPISKDLYEELPKNRGKLFTPCRKAFERAVKRAGIDLP
                     EGQCTHVLRHTFASHFMMNGGNILVLRDILGHSDIKMTMVYAHFAPDHLEDAVTKNPL
                     FNLR"
BASE COUNT        11395 a        13009 c        11410 g        11803 t
ORIGIN      
        1 taatgagcat tctgaaaaaa tatcgatgca tttcgagcga agatggtggg gatccctgaa
       61 tgcgtttttc tcaccgactg ttcttactgc tgattctcct gctgacgaga gcaccaatac
      121 tcgctcagga accttctgac gttgcgaaaa acgtgcgtat gatggtttcc ggtattgtca
      181 gttacacccg ctggcctgcg ctatcagggc caccaaagtt atgtatcttt tcatcttcac
      241 attttagtaa cgccctacaa gagaatggca cgacatcttt gccttatcta cctgtcatca
      301 ttcacactca acaagaggcg atgatttcag gctgcaatgg tttttatttt ggcaacgagt
      361 caccgacatt ccagatggaa ttaaccgaac aatatccgtc aaaggcgtta ttattaattg
      421 ccgaacaaaa taccgagtgc attattggta gcgccttttg tctgatcatc cacaataatg
      481 acgtcagatt tgccgtaaac ctggatgcct tatcgcgtag cggtgtaaag gtcaacccgg
      541 atgtcttaat gctcgcacgg aagaaaaatg atggataacg ataattctct taataagcgc
      601 cccacgttta aaagagcatt acgcaacatc agtatgacca gcatatttat cactatgatg
      661 ctgatctggt tgctgctttc cgtgacctcg gtgctgaccc tgaaacagta cgcgcaaaaa
      721 aacctggcac tgacagcagc aacaatgact tacagtctgg aagcagctgt cgtttttgcc
      781 gatggccctg cagcaactga aacactggca gcgctgggcc agcaagggca attttcaact
      841 gcagaagtac gtgataagca gcaagatatt ctggcatcct ggcattacac ccgtaaggat
      901 ccaggcgata ctttcagcaa tttcataagc cactggctct tccctgcccc catcattcag
      961 ccgattcgtc acaatggcga aaccattggc gaagtacgct taaccgctcg cgacagttca
     1021 atcagccatt ttatctggtt ttcgctcgcc gtactgaccg gttgtattct gctggcatca
     1081 ggaatcgcaa ttaccctcac ccgccatttg cacaatggcc tggtggaagc actgaaaaat
     1141 atcaccgatg tcgtacatga tgtgcgttcc aaccgcaatt tttcccgacg agtttcggaa
     1201 gaacgtatcg ctgagtttca ccgcttcgct ctcgacttca acagtctgct ggatgaaatg
     1261 gaagagtggc agcttcgttt acaggctaaa aatgcgcagc ttctacgtac cgcgctacat
     1321 gacccattaa ccgggctggc taaccgcgca gcgtttcgta gcggcatcaa cacgttgatg
     1381 aacaattccg atgcccgaaa aacgtcggcg ttactatttc ttgatggcga taatttcaaa
     1441 tacatcaatg atacctgggg tcatgcgacg ggcgatagag tcttgattga aatctcaaaa
     1501 cggttagctg aagttggcgg gctgcgacat aaagcatacc gcctgggcgg cgatgaattc
     1561 gctatggtgc tctatgatgt acagtcagaa tctgaagtgc agcagatatg ctcagcactg
     1621 acacaaatct ttaatctccc gtttgatctt cataatggcc atcagaccac catgacatta
     1681 agcattggtt acgcgatgac cattgagcac gcctctgcgg aaaaattaca agagcttgcc
     1741 gatcacaata tgtatcaggc caaacaccag cgtgccgaaa agctggtgag ataacaagga
     1801 tatatcgata tgataaagca cctggtagca cccctgattt tcacctcact aatactgact
     1861 ggctgccagt cccctcaggg aaagtttact cctgagcaag tcgccgctat gcaatcttat
     1921 ggatttactg aatccgccgg cgactggtcg ctgggcttat cagatgccat tctgttcgca
     1981 aaaaatgact acaaattgct cccggaaagc cagcaacaga tccaaaccat ggcagccaaa
     2041 ttggcctcga cagggctaac acatgcccgt atggatggac acaccgataa ctatggtgaa
     2101 gacagttaca acgaaggctt atcattgaaa cgggcgaatg tcgtggccga tgcatgggct
     2161 atgggtggac aaattccacg cagcaatctc accacacagg gtttaggaaa aaaatatccc
     2221 atagccagta acaagaccgc ccagggccgc gccgagaacc gccgcgtcgc agtggtgatt
     2281 acaacccctt aaaacacgag caaacagaca aaaaaagggc cagccaattg gccagccctt
     2341 cttaacagga tgtcgcttaa gcgaaatctt agttaagacg ctctttgata cgagcagcct
     2401 taccagtacg ctcacgcagg tagtacagtt tagctttacg aacagcacca cgacgtttga
     2461 cagaaatgct gtcaactacc ggagagtgag tctggaagac acgctcaacg ccttcgccgt
     2521 tggaaatttt acgaacagtg aatgcagagt gcagaccgcg gttacgaata gcgataacca
     2581 cgccctcgaa tgcctgcaga cgttttttgg aaccttcaac aacccatact ttcacttcca
     2641 cggtatcacc cggacggaag gaaggtacgt cctgcttcat ctgctcttgt tcaagttgct
     2701 taataatgtt gctcataatt taatctctta tcctgggtaa actgatatct cgggggctta
     2761 cgccatccca tcatgtttat gttgctgttg tgcgtgttcc gttttgaact ccgccagcaa
     2821 ccttgcttgc tcttcagtca gagccaggtt ttccagaagt tcaggtcttc taagccaggt
     2881 acggcccagc gactgtttca aacgccagcg acgtatctcg gcatggttgc ccgacagtaa
     2941 cactggcgga acttccatcc cttctaacac ctcaggccgc gtatagtgcg ggcaatccag
     3001 caatccttca gcaaaggaat cttccgttgc cgaggcttca tgtcccagta ctcccggaat
     3061 aaaccgggaa acggagtcaa tcagcgtcat tgctggtaac tcaccaccac tgagaacgta
     3121 atcgccgatt gaccattctt cgtcaatttc ggtttggatc acgcgctcat ctataccttc
     3181 gtagcgaccg cacaccagaa tcaatttttg attcgttgcc agttcgctga cgcccgcttg
     3241 atcaagcttg cgtccctgtg gtgacagata aatcaccttt gcgccttcac ccgccgcggc
     3301 ttttgctgca tgaatggcgt cccgcaaggg ttgcaccatc attaacatcc ccggtccgcc
     3361 gccgtaagga cgatcgtcca cggtacggtg ccggtcatgc gtgaagtcgc gaggactcca
     3421 gctctggatg ctcagcaggc catttttaac tgcccggcca gttaccccgt aatcggtaat
     3481 tgcgcggaac atttcaggaa acaggctaat tatgccaatc cacatagcgc cgtcttttac
     3541 cgtttatccg gtggtttaaa aaccaggatc ccaatctact tcgattgaac gagtagtgag
     3601 atcgactttc ttgataacct gcccatcgag gaacggtacg agacgttcct tgataccaaa
     3661 cgcatctttc aggtttgcct taatgacgag aacgtcatta gatccggttt ccatcatatc
     3721 gacgacttta ccgagatcgt agccttcagt ggttactacc tggcagccca tcaggtcttt
     3781 ccagtagtag tccccctctt caagctgagg cagctgcgat gaatccacga caatttcaca
     3841 attcgtcagc aggttcgccg catcacgatc gtcaacgcct ttcagcttga tgatcatgtc
     3901 ctgattgtgg tgcttccagc tttccagctg gacttgctgc cactgacccg ccttctggat
     3961 aaaccaaggc tgatagtcaa aaatgctttc ggcgtcttcg gtggaagaaa acactctgag
     4021 ccacccacga ataccgtaag acgaacccat ttttcccaaa acgatgggat caacaggtgc
     4081 ttgcgcggtg agttgtttgc tcatcatgac caccgtgaca gattaagctg ctttgtttac
     4141 ttctttgatc agcgcagcaa cgcgatcaga aatagttgcg ccctggccaa cccagtgagc
     4201 gatgcgatcc agatccaggc gagtgccttc ttctttttcg ctagcgattg ggttgaagaa
     4261 accaacgcgc tcgatgaagc gaccgttgcg tgcattacgg ctgtcagcga caacaacctg
     4321 gtagaacgga cgctttttag cgccgtgacg tgctaaacga atagttacca taacatcctc
     4381 ttgtgtgaat aaaacaaccg gaccccatcg aggaacggag tccggtgtca tattaaaagc
     4441 ccgaaaattt tactcatttt tgcgggaatt gcaatcaaca gttgctaact ctgctgtaaa
     4501 aggccgtcgg cggtgcagcc agtttggtgc cggagtgcgc gcagtcaccg gagcgtacac
     4561 gcagtacgtg aggatgacga gcacatcccg gtgccaaaat ggcaaacaag ccaggccgat
     4621 tagcgaccag ggaagcctgg gggcatcata cccttcatgc ttctcatcat cttcgccatt
     4681 ccgcctttct tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg
     4741 ttcacgtcct gcacctgcat accgcaaccg gcagcgatac gacgcttacg cgaacctttg
     4801 atgatttctg gcttagcgcg ctctttcatc gtcatcgagt tgatgatggc ttccatacgc
     4861 accagcactt tatcgtccat ctgcgactta acattatccg ggatctgccc catgcccggc
     4921 agcttgccca tcaggctagc catgccgccc atatttttca tctggcgcag ttgctcaaga
     4981 aaatcgttga gatcgaagcc gtcgcctttt ttcagcttgc tggctaattt ctctgcctgc
     5041 gcgcggtcaa ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga
     5101 atacgcgacg cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca
     5161 ccgagaaact tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg
     5221 gcatcgccgt ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct
     5281 tttgccgtat tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc
     5341 gggttaatcg acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga
     5401 cgaccagcgg tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc
     5461 gcgttaacga tatctaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc
     5521 tgctctgcca gcgtctcaag ctgtttgatt gccgccgggc gataaacgtc ggcagaaacc
     5581 accagcactt tcttcttgtg cttctcgcgc aggaacttac cgagtttacc aacgctggtt
     5641 gttttaccgg caccttgcag gcccgccatc agcacgaccg caggcggttg cgccgccagg
     5701 ttcagggtct ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg
     5761 aactcctgcc ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg
     5821 cgattgataa actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc
     5881 acttcgcgca gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg
     5941 cgcagcgtgc gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg
     6001 aaacggttgg ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc
     6061 gtctttgttg ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt
     6121 cttattgctc aaactgtcga catcactatg cccgtttttg ctctgctcgc gcttgtcgcc
     6181 tactccgtca gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg
     6241 cgcatggcta ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc
     6301 cgcatcctgc ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg
     6361 gtcagtttga tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg
     6421 ctgctaccca ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc
     6481 aatgaataca tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg
     6541 ctcttttcct atgccacgct gataattgcc gccctgtacg cgctacaact ggcgtggatt
     6601 gattaccaac tgaagaacaa gaagctggcg tttaaccaag aaatgccgcc attgatgagt
     6661 atcgagcgta aaatgttcca catcacgcag attggcgtgg tgctactaac gctcacgctt
     6721 tgcactggcc tgttctacat gcacaactta tttagcatgg aaaatatcga caaggccgtg
     6781 ctctctatcg tggcgtggtt tgtctatatt gtgctgctgt ggggacatta tcatgaagga
     6841 tggcgtggac gccgcgtcgt ctggtttaac gttgcgggtg cagtcatcct gacactggcc
     6901 tacttcggca gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc
     6961 tggaacacat ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag
     7021 cctatttttc cgggtccgaa accggaatga tgaccctcaa ccgctatcgt ctgcgacata
     7081 tggcgaaaca gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc
     7141 gcctgataag cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg
     7201 gcactattgt tgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc
     7261 tgacttttgt cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctatacc
     7321 cggaaaaagt cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc
     7381 cgctggtctg gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa
     7441 ccgatatcgt ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcacgaat
     7501 cacgctcaca aatttcccgt cgcaatcagg atatgctact gtctgtgctc gatctggaaa
     7561 agatgaccgt tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg
     7621 atgactggaa atcgattctg cgccaactct cccactcacc tcacgggcgc atcgtgctct
     7681 accgtgattc gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga
     7741 tgtcggagaa aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt
     7801 ttgtgccgga aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga
     7861 aagtcggcct ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata
     7921 ttctggaaga gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg
     7981 tcacgccgca aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca
     8041 acaaagcctt taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc
     8101 ttgaggcact ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata
     8161 tagatattct cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac
     8221 cgctgcgcga gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg
     8281 aattattacg aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga
     8341 atcgtacgac cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca
     8401 acgtcatcag attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta
     8461 gtttcggcga tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc
     8521 agctcaatgc cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc
     8581 gcacgatcca ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat
     8641 ttgtgggctt tttcaatatc cagttcagta cgtcgacgca ggttttccat ttcggctttt
     8701 acacgcaaaa tgccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc
     8761 gcaatttttt catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca
     8821 atctcttcgt gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt
     8881 tctttactac tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta
     8941 ttatggggat cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa
     9001 caaggacctc ggaaaaatga ataatcattt caagtgtatt ggcattgtgg gacacccacg
     9061 gcaccccact gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta
     9121 cgaggtcatc gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg
     9181 cacgctcgcg gagattgggc aactagctga tctcgcggta gtcgttggtg gcgacggtaa
     9241 tatgctgggc gcggcacgca cactcgcccg ttacgatatt aaagttattg gaatcaaccg
     9301 tggcaacctg ggtttcctga ctgaccttga ccccgataac gcccagcaac agttagccga
     9361 tgtgctggaa ggccactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca
     9421 gcaagattgc cagaaacgca tcagcaccgc gataaatgaa gtggtgcttc atccaggcaa
     9481 agtggcgcat atgattgagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg
     9541 atctgatgga ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcagg
     9601 cggtcctatt ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac
     9661 gttgtcagca cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca
     9721 tcgccgtaac gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg
     9781 tgaagatgtc ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta
     9841 cagttatttc aacacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta
     9901 cgccagcctc tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt
     9961 tttcatacag gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc
    10021 gtgagcttga gattgatttt catagcggca tgaccgtaat aactggcgag accggcgcgg
    10081 gtaaatctat tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca
    10141 tggtgcgtac cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc
    10201 cagcggctct gcgctggctg gaagaaaatc agcttgaaga cgggcatgaa tgtttgcttc
    10261 gtcgagtgat cagcagcgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc
    10321 tgtcacaact gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc
    10381 aattactcac caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct
    10441 ctctactgca ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg
    10501 cgcatcatca acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat
    10561 taaaagaact taacgaattt aatccgcagc ccggagagtt tgaacaaatc gacgaagagt
    10621 acaaacgtct ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa
    10681 tggccgacgg tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga
    10741 gcgaattgat tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta
    10801 ccatccagat tgctgaagcc agcgatgaac tgcgccacta ctgcgatcgt ctggatctcg
    10861 atcccaaccg actatttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta
    10921 aacatcacgt cagccctgag gcattgccac agtattacca gtcgctactg gaagaacagc
    10981 agcaactgga cgatcaggcc gactcacaag aaacgcttgc gctggcggta acgaaacatc
    11041 atcagcaggc actggaaatc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag
    11101 aacttgcaca gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta
    11161 cgatcgatgt taaatttgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt
    11221 ttcgggtaac caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg
    11281 gtgaattatc ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc
    11341 cggcactgat ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg
    11401 gcaaactgct gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac
    11461 aagtcgcggg atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga
    11521 cagaaacgca tatgcaatcc ctgaataaaa aagcgcggtt acaagagctg gcgcgcctgc
    11581 ttggtggcag tgaagtcaca cgtaatacac tggcgaatgc gaaagaactg cttgcagcgt
    11641 aaactttttt cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca
    11701 aaaggtttta aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac
    11761 tgctcgggcc cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca
    11821 gcagtactat tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct
    11881 gacatcaacc aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg
    11941 acgcaacaac aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg
    12001 aatacctggt tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg
    12061 ctgacgctga cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg
    12121 agtggtaact aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc
    12181 ttttttctgt ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg
    12241 gatcggcaat gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta
    12301 gttttgccgg acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca
    12361 attccagcaa gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca
    12421 cgcgctgcag gtactgcttc tcaggtagcg cataagccac ctcaacggca attttacctg
    12481 gcactgtaaa cctctttcgc acgaaccgta aaagcctgga ccatattaga cgccagctct
    12541 ttaaacacgc gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga
    12601 aactcgatac gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc
    12661 ttgaacggcc catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg
    12721 gtagtaaacg ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca
    12781 ggagtggact ccagaatccg acttccggta caacccggca aaaactgagg ataagactga
    12841 acgtcattca ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta
    12901 atctgaggca taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct
    12961 gtaaaaaaaa cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg
    13021 tcccccgcag gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct
    13081 tatgacgaag aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc
    13141 ccgtcacgaa tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga
    13201 ggttaaatcc ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga
    13261 cggagaggca tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt
    13321 ggtgtgcgat cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt
    13381 gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa
    13441 tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc
    13501 agatatcaaa gaacgcgaat ggcaggtgga taaagcacgt atcatgaaaa acgcccaccg
    13561 ttaaacctgc actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg
    13621 aatgaacatc ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg
    13681 aataaaaatc aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca
    13741 tggcgctcat aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg
    13801 ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa
    13861 aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct
    13921 ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg
    13981 tggaagccct gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt
    14041 gtccgtccgc agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt
    14101 aggaatttcg gacgcgggtt caactcccgc cagctccacc aaataaatca aggggttacg
    14161 tgaaagcgta gccccttttt ctttggtagt ggcggcaaaa tggcgacaga cttttgcgtc
    14221 catcttgcct gtcgccatct tgaaatcatg caaagaggtt tcacatggaa gaacttcact
    14281 ttgtttacat caatgcaaat ggtcgtatcg gtgttcactc tatacagagc atcagttata
    14341 gcgaaaatca tatacagggc atttgtaaga acaccgatcg aataaaaacc ttccgaaaag
    14401 accgcattct taaacagtac gattcaccag aacaagccat tcaggagtgc gcgtcattcc
    14461 tccccgaaaa ctactcacat ctcaccaagc agtctggtcc gaaaaaaaat acattcgatg
    14521 tatgtttcac cggatttaag aaagcagata aagaaagatt ggttgataag gcgaatgaac
    14581 aaggattaac ggtaagaacc tctgtaaccc aaagccttca gatgctctgt tgcggttaca
    14641 atgcaggccc atcaaaagta tcggcagccc ggatgaaagg cacaatcatc atagatgagc
    14701 ctggctttat acattttctt gaaacgggtg agatcccaga tgaataaaaa cctgccgtag
    14761 caggttctct ttctcaaaaa ttcatatgcc cctgaccacc tggcaatgga tgtggaggag
    14821 cagtagcaat cagtgcgggt gtcacaataa accggaccac tgtttcatgg gtaacaaaag
    14881 tgctcccgca gttaatattt tggcactggc agtaacgctc tttggtgctt tcagttactt
    14941 gaaaactgct ccttgtgtgt gccgcatgac cacactttgg acaattcatc atatccagat
    15001 ccctaccttt gctatcagaa tcattgtaat gatacacaaa atatcaatat tgagaacact
    15061 ttattccatt tcaagatcat caatcttcac ttcgagttca atactggttg taaaaccgtt
    15121 atccgggctg acggtatgtg tcagagtcgt aatggtccat tccgcatcat ctatcggctg
    15181 tttaaaacca ctgaccttca caggcatttc cgtgtagaga tctgcccgcc cttccgccag
    15241 ttgtagcgag aatgacgcaa cgccacgttg caggcgttcc cactgcattt tcgctgcccg
    15301 ttcagcgttg ctccggttgg cataagtgcg attaagtacc agcacgtttt catccgtccc
    15361 caccaggtaa tcgccctgct tcgcttccgg ctctttcttc tgcttcttag tcctgcgctt
    15421 acgcttcacc gtagtgcttt ctttcttcgc gggttcgcgg gtatgcaacc agctggcaat
    15481 tacccccgtg taagctccgc gatctgccag ggtaaagcgg tgactgtcgc cgtccttacg
    15541 tgtgatagta atcaccggca gtggtttacc gctggcgctt ttgccctgtc cctgccggat
    15601 gaataacaga ttgccatttt tcaccgatgc aatggcaccg tactggcgcg ccagccgcat
    15661 caaaaaactg ccgtcactct cattagtctg gtctatatgc tccacaggct tatccgacag
    15721 gtctttaccc agtgccatct tcagcttgtg ccgcgcggct atttccttca ccacttcccc
    15781 gatggtggtg ttatgccacg atttttcacg gcgggtattc agcgtttccc gaaaatcagc
    15841 acttcgcgcc cggatagtca ggcggtccgg tgcgccagtg tgttcaatct cgtccaccgt
    15901 gaatgcccct ttcgggaaaa gcggctgccc cttccagccc agcgccagcg taatgaccgc
    15961 accacggcgc ggcagcacga tttttccgtc ggcgtcgtcc agctccagat caagctggtc
    16021 cgcttcaaag ccccggttat ccgtcagcgt aagccccatc aggcgtttgt ccagcacagt
    16081 ggtgatatcc ttcccctcaa tactgatgct gaatgcggga gttttgttgc ctttgttaag
    16141 cagttcagag ctgaaattca cgacagcagc cctcccaccg ttttactgat atcgcttaag
    16201 gcagacgttg ccgtatcctg caaattattc agctgcgcac tgagatcacc gaacatatcg
    16261 gacagggatt catccacccg tttgagcgac agggtaaact caatccggcg cggcatacca
    16321 tcgcggaaaa actccgtttt agtctgattc agtccctcaa tcacatacat gccgtaaatc
    16381 gtgccgctgc cttcaatcag gggccatgct tttccctgtt ctgccatctg ctccagagcc
    16441 agcaacgaca gcctgccgcc cgttatctcc ggcataagaa caccagaaag cgtcagcatg
    16501 tcgttgtccg gtcccagaaa ctgcgtggac ggacgacggt ttacccggct gtttgccgca
    16561 tgtcgccagc tgcgttgata ctgcagttcc tgatacggaa cggtgcgcag cataaacacg
    16621 tacaatccca gcaccatcat catgcgtcgt atcccccctg atcgctgtag ttactcctgg
    16681 cttttgcctt cagcctgcgt tcacgttcat caagctggcg tgccacctcc cgcgcaatat
    16741 cctgcgcact ttgtcctggc tgcgtctgaa tgatgatctg cgtcggtgcc tcaatccggt
    16801 gaacgggcgg cacagtggct gcacgactca ccatcgcttc gccgcctttc gcgggaagtg
    16861 ccaaaggatg caacggtgga agctctgcag gcgcggcagc aacgcccatc attccggcaa
    16921 caacggcagc cagtgcagct gtatttctcc ggctggtcac atttgccggg ccgttaacaa
    16981 tttccggccc gttttcaccg acgatgccaa actgcccacg cgggatatag ccgccgctgt
    17041 catacatccc cgcaaagcca tatccccatg acggaaaacc ccccgatggc atcatcactt
    17101 taccgtctgc attcaccgtc gcaggttgct gacgcgtcac tctttccggc agtttcgcct
    17161 ttgcagcctc tttactgaca atgccgagtt tctccagcaa ccaggaaacg ccggatttca
    17221 gggagtccag cggatgcatg accatattca gcccttccgc cagtgcctcc ccgaatcgcc
    17281 gccccattgc cgctgcactc tgcagttcgg cagaggtcga cttaacgggc gtcagcagat
    17341 cagtaaacca gccccacagc gcctgtactt tgtcgccaat ccactggaac acgggcttaa
    17401 gcggttcgaa tgctgcactg acgggacctg ccgccgcttt gaatccttcc accacgccac
    17461 caagaaatgc ggtgatgggt tgccagtatt tccagacaac cagcgccacg cccgccagtg
    17521 cagtaaccac aagacctatc ggactgagca gagcacctaa cagaccagat acggcataca
    17581 gggcaacgcg cagcatcgcc agtggaccag atgccagtac tcgcagcacc gtgcctgcgg
    17641 aggccagtcc accgcgcagt accgccagag gattcataaa catcacagca acagcacgta
    17701 aaccggataa tccagaccgc aaaagtgcaa ccggcgcacc tgctacagtt ttcaggacat
    17761 ttcccgtcag tgatgccgtg cggcgcaaag acgacaacgg cgcagtaagt aaacctgcgg
    17821 cgttgcccga tgaagcaagc ccgcgtcgca gcagtgcaag tggtgcgcca gccaaccagg
    17881 acaacgcgct gctggttcgc gttactgctg ccgtaacgga aggtaacgtt ttgataccca
    17941 gcacagagaa tcccagacgg atgactgcca gcggccccag cacagcagcc aacaccacag
    18001 ccagggtgcc gaggcccaca gtaacagcag ccacaaccgc ggctactttc atcagtgtgc
    18061 ccgtcagttc agggttagct tccacccagc gacgcaacgc ccccgtgatg cttttcaccg
    18121 tgtacagaat atccatcagc ggctggcgca gcgtttcgcc caggctgctg aaggtgttct
    18181 gcgctccggt tttgaccaac aaccactgcg cagaaagtga gtccttgtta atgtcggatt
    18241 ctttctgcat cgaaccgagc gcatcattgc ccgctgtcag ttttagctgg cgctgcagtt
    18301 ccggcaggtt gtttgccagt ttcgccgcat catcgccaaa ctctttacca aacaacatgg
    18361 tcatggcaga caggcgctta tcctgcggca gtgcgttcac cttctccagt acgcgctgga
    18421 tagttcccat cgcatccttc gtcatctgct tttcaatcac ttcaggattg agtttcagca
    18481 gattcattcc ttcaaagaaa ctcttgcttt gcatggtcgc aatggacaat tcacgcacca
    18541 tcgcgttggc agcactggca gcgacctccg gcgcagcacc cagtgtcagg aaggtggaac
    18601 ccagcgccgc cgctttacga taatccagac gatcagccac accgcccagg cgttgcatga
    18661 catcaatgat gtctgcccct ttcgacatag cgttatcatc cagatagttc agcgcatcac
    18721 cgagctgttc aatattgcgg gttggtattt tgtagagctg ggcgattttc cccagacttt
    18781 ctgacagttc atcagctggc agctcaaagg ctgttgccgc ctttgctgcc gtactggcga
    18841 aggccagcag gtcacgtttc tggtcctccc agctgtcgtc agggtttgcc acattcatgc
    18901 gcgcaccacc ttcaaccagt gcggcgaagt ccacagcacc gttttccatt ggcagctgtt
    18961 cactggcagc tttgatggca tcctgcattt cgtaaaaacg cgcagtgcgg ttgccattat
    19021 cgtcacgcag accattgacc tgctttgcca cacctttcat ggcatcttcc atgctggtat
    19081 agctttttac cgccgccatc actggcgcgc ccattgccag ccctgcagcc gtggtggtgg
    19141 ctccggctcc tgcgatgcga tcgcgcactt caagccgtct tgagtattgt tctctggcag
    19201 cgttcatccg tgcctgttgt tcacccagac gtttaagtgc ttttttctgg ccctccagtg
    19261 cctgacgagt ttcttcggca tttttcttaa gttctcgctg ggcactactg agttgtctgg
    19321 tatcaatccc tgattcttta agtgcctgac gttgtctctg gaccgccccc aacaagccgt
    19381 tataggtctg ctgaagttcc tgtactcgtg ttttggcctg actgaataac tttgcctgcg
    19441 cggcggttgg cctgttagtg gcagcaaatt gtgtggcgag ttttgccgcc tcttcgcggg
    19501 ctgcgttcag gttgttggct gttatggcta gttgcgagcg cgtcttgcga aattcatcaa
    19561 ttctgccagc ctgcttattc agttctttga ggctgtttcg ggtattctga attgcgccag
    19621 ccagctcttt cgaactggcc tgtgcagcac ggaatgggcg ggtgagtttg tcaaccgcat
    19681 taagaatgac ctgcaggcgc aggttattat cactcatcgt tggccccgct tctctgaatc
    19741 gctttatacc gccattccag cacttcggtc agcggcataa cgtcagtaac ggatggcggc
    19801 cagtgaaaga tggtggcgat atcagccacc agatcgtcaa ccgtcaggct gtcggtaaac
    19861 cggcaagcac cgacttcttc aacaaaaaag tgacaacctc aaccgacatg gcagtgagat
    19921 ctgccgggtc catctctgca atttcctgtg cagtcagtgc cggactggag atgcggggga
    19981 tcacggtcat catcgcgttc acatccatat ccataatggc ctgcaggcgt gtaccgcgca
    20041 gcgcaccgga ctgcggttta cgcagcacaa tttcggtgat ttctgtttta ccgcgcttga
    20101 tgggggtatc cagttgaata gtcttttcag tctgcttatc gctcattttg ctgtcctgta
    20161 aattgggttc tggcgcggta tcccgcgccg ttcagataca tcagaggcag ggcgttgcgg
    20221 tgcgcttcca tcaggtccac accgtccaca atttccacca tgttgataag gtccacttca
    20281 tagagcacct caccatttat agtcagcttc gcgtagctgt tggtactggt cactttggtg
    20341 gtgttgcttt cgcccgtctt ccactcgccg gaatccactt ctttgtgacg tccacgcacc
    20401 acaagctcca cggcctgcac ttccccggta tcgtcacgct ggatagagcc ggtaaagcgc
    20461 aactggatac catccaccgt ggctttgccc atctgcttaa acagcagcag ttcagtacca
    20521 ccaatggaaa attccgtatc cagtgcgccg tcatccagcc ccagatccac atccactgca
    20581 cccggcattc cgccgccgcg atacttctca tatttgcggg taaatttcgg cagcgtcagc
    20641 gactcaacga tcccctgcca gttgttcccg tcattaaaca ggttcaggtg ttttaatttg
    20701 cgtggtaaag ccatgttgtc cccttacgcg ctgacctggc tggagaaatt aaccaggtac
    20761 tgatcggtga tgcgctgacg cagcatcagg ttttcaagtg gcggcactgg cgtgtagtcg
    20821 tagtcgatgg tgagttttcc ggctttcagc gtgtctttgt cgttcaccga ctcatccagc
    20881 cagcaatcac caccaatgag atagccctga ctgaccaggc tgcgcatttt ggcgcggata
    20941 ccctcgataa tgtcgcgggc cagcgacgga ttcagcggtt tatccaccgc ccacatgtgc
    21001 gcttctgcca tcgtgtccgt cagcacctgc gccgtgcggg tgtagttttc gaaggcaaag
    21061 agcgggtcat cactcaggca gcgggaaccc cagaagcgaa aaccgtcttt gcggataagg
    21121 gtggtgacgt cgttctggtt cagcagtccc gcatcggttg ccgggtcctg cagatcccag
    21181 aacacatcag cagaaattcc ggtgacaccg ttaacgccca cgttggacag gcttttgtgc
    21241 catccggtct gctcgtcaat tttggcgcgc agaccaagcg cacgggcggt ggcataagcc
    21301 gttgcttcgg cattcagcac cgtgtcccag ccagtaaagt cgggccagat cagcattcct
    21361 tcacgctgac tgaagttttc acggtaagtg attgcttcct gcaccgtctt gcaaccatac
    21421 gctgacaggt aagcaaatcc acgcaggctt tgcgccacac tcagcaactc agtagctacc
    21481 gccttcgtgt cgtgacctgg cacgccgaga atgcgcggtt taacgccgag ctgtgactgg
    21541 gcagataaca gggctttcat gcctgttttt ttaccttcag cggtcactgc gccgatgata
    21601 ttggtcgtgg tttcttcttc cgtttcaccc tgcggcacac gcacaacaac ggtcacgggt
    21661 tttgcctggt cagcgatggc atccagcgaa cgagccagcg tgcctgactc acccgcttta
    21721 ccgctggcag tcagcacatc agtgatcagc acgggtttat taagaggaaa catttttgca
    21781 tcggcatcat cgcccgtgca gaccataccc acgatggcgg tgctcaccgt ggtaatggat
    21841 cgggtgcctt cgttgacttc aacaacgcgc accccgtggt ggtaatcctg agccatagtg
    21901 gcgaacctcc tgattggatt aggcttcgcc ctatgttgaa gtgattgtgc ctgacaaaca
    21961 gctaagcgca gttgtaccgt tattcacaca aaatgacggt atttgtctgc tttcagggat
    22021 aatcaaaata aagctgattc agggagattt attgttctta tttgccggaa attttcgata
    22081 aatggtagaa acgcctacat caaaaatcag tgcaatacgc tgtcttgatt ctccgtcctc
    22141 gagtaaacgc ccaatctgtg cccactgttc gctggtcaac ttaggacggc gtccacctac
    22201 tctgccttta gcacgagctg cagccagccc cgccctggta cgttcaacta tcagttcgcg
    22261 ttccatttca gccagggcac ccatgacatg aaaaaagaaa cggcccattg gggtgctggt
    22321 atcaatactg tcagtcaggc ttcggaaatt cacgccacgc tggcgcagct cttctatcag
    22381 cgtaacaaga tgccgcatac tgcgccccag tctgtccagc ttccagacaa ccagcgtgtc
    22441 tcctgccgat agtgtcctga gcagcttttt cagccccggt ctgtcggact tggtgccact
    22501 gattttatcc tcaaaaatcc gctcacatcc cgcacagttc agtgcattgc gttgcaaatc
    22561 ggtgttctgg tcatttgttg acacgcgtac atagccaata agcatgatca tccccctgaa
    22621 taaaaaccgg agatgatgcc agttagccgt tatctctgca ttttcataaa cgttggtttg
    22681 ggagaagcgg caaaacgggg tgtgggaaca ggggaaaatc agataccgga catggcttca
    22741 tttagtgggg tgagggatta ttacggaaaa caacttttgc caggagggtt gatacttcag
    22801 tggctgacga ttccatcaag tgcagcagcc aaagctgtaa cactgaataa tggtaattat
    22861 cagctgtcag gctataaatg gccccagtca tttggtgtcc tgtttgctgt gtttgctaca
    22921 aaagtttctg gctcgactaa cgaagcatat gcaatctcag ttaatcgtca ctctaccgat
    22981 gtaattgtca cctggaatgc ccgtaaggct gatgatgtcc acattttagg aattgggaaa
    23041 ttatgaaaat gaaatggtcc ccatctgttc agggattttt ctctgaaaat aacagcgata
    23101 ttcccgatga tgctttcgat attgaagatg ctctttatta tgaacttatg aatggtcaga
    23161 gtacggggaa aattattatc aataacccgg ataactaccc tgtacttact gaatatccag
    23221 cgaagacaca ggaacaggaa atagctgaag cggagggaat gaaaagtata cttattgaac
    23281 aggccaacga atacatgaac agtaagcaat ggcctggtaa agccgctatt ggtcgtctga
    23341 aaggtgagga actggcgcaa tataatttgt ggctggatta tctggacgca ctggaactgg
    23401 ttgatacctc cagtgctcca gatattgaat ggcctacgcc tccggcagtt caggccagat
    23461 gacatccggc gcagtgctgg tatctgttgc cgtcaccgcg tcaatgtaat ccagcacggc
    23521 gttaagtcgg gtggtttctg cctgcgtcag cttccgcccg gcctgtaatt tcagttgaat
    23581 cagactgatg gaagccattg cagcatcaat cagcgactgg cgctgtgctt ctgccgcttc
    23641 tactgcggcg ctatgctgtg cctcggtatc cgtcacccat ttctcaccat cccatttatc
    23701 gtatggcgtt aacgggacga tagtggttgt attttcaggg taattacccg gagctgtgat
    23761 ttcttttgat tctcccgttt cggtgttata gacaacttca ccgcgatggt ctggcacata
    23821 ttcccatgag tttaaatctg ccgaacggca gattgcataa ccagccttat gtgttccagg
    23881 agcatctaaa caggaacatg ccgggatacc gacacccaca gcaagatatt cagttgatgt
    23941 ggaaatatat tctcgtgtct caccatcata attgtaaacg gtaatgtctc ccgcctttgt
    24001 ggtgatgagt tcgctattta atacagcttt attcatcatg cggccctcac aatataatta
    24061 aaggcgatgt tacgtggacg attttcattt gctgttggaa caacatttgc cgccgagaag
    24121 tgaacatctt gtcggatttt tcggtcatta gcgtatgtca tagatacgtt gatgttattc
    24181 cggactgagg tacctccgta aaaagcacca ctcacacttt gaacatcaac aattgcattt
    24241 atttcgctgt ttacgcgcaa ctgaccaaaa ccgccagtaa tgtttcgaat agcatctcct
    24301 tgcgctgaaa gcagagttcg cccactatcc acaccacgtt catcatccca gccacgaata
    24361 aactcaccac gtaaatcagg caatttattt gtcggataag cctttgccag ttccgggtat
    24421 tcttcagcag aaaaagctgc accgttgcat ttcagccagc ctgtcggcgg tgtggctgaa
    24481 ggccacggaa caggcacccc aaccggtaat gcagagcctt ctcccaaacc aaggtatgcg
    24541 agaagaccag ctacatcctt tccactcaaa ttagtcagcg tattgtccag cggttgttta
    24601 cctgctagcg cattaagcat tgtcgtggca aagttcgggt cattccccag cgccgccgcc
    24661 agttcgttca atgtatccag tgccgcaggt gcagaaccca ccattcctgc aatcgccgat
    24721 ttcacaaaag ccgtagtggc aatctgtgta ttgttgaccg actgcgccgc agtaggtgct
    24781 gttggcgttc cggtaagtgc cggactcgac agcggcgctt tcagtgccag cgcattatta
    24841 atagtggtac tgaatttcgg gtcattgtta atggctgcgg caatttcttt cagtgtgtcc
    24901 agcgtggctg gcgcaccgtt aatcagagca gtaatagcgg cctgaacaaa ctctgtggtc
    24961 gcaatccgag tggtgttatt tcctgctgca ggcgtcggcg cttttggttc tccggtaaat
    25021 gtcggattat gtttctgcgc atactgggta tgaggatctt gtgcggcaat gtggtttctc
    25081 atctggtcat ccacatacag ctttaattcc aggactttat catccacgta tttacgggtt
    25141 gccagcacta cagcagggtc gattttcagg atgatattgt ccgtgctgct ggtaatcagc
    25201 accatgcgca cggtctgggt acgcccgctg ccttcagcca gttgcggctt atagctttcc
    25261 ggccagtttc ccacggcaat caatgccccg gactcatcaa acaggcccac ttcacgtatc
    25321 caccaaccgc cctcgttttc agggatcacc tgttcagcaa taatctggct actgttctgc
    25381 gggtcgatat aaagcatatt caacgcagcc cggcgtttct catttaccag tgccgtctgc
    25441 tttgcgtccg gcgtcggtaa tactccgccg ccatcgccga ccgccatatg ggtaattttt
    25501 agcggcacac cgagcgcggc ggcgctggca agtttcgccg cgccaatatc cgtcagcagg
    25561 gtataaaatt ttgtgctcat ggattcactc tcattgtgtc aataacatgg accgccccgc
    25621 cttcatgcgc ggtgccgcca gaaataattg tttcgttgat atacggatag atcgtgattt
    25681 cttcaccaag atagctggcg gcccctaccc agtgcgggcc actggtctgc agattgatgg
    25741 acatgccgat catgtgacgg ctacatggtt tggcatcgct tatcagccgc tcaagttcca
    25801 gataggtatc ttcagtgatg ccctgatcct gcacgccgat atccaggcga aacgtgcctg
    25861 gtgtttctcc ggtctgccac cactcaataa tgcggatcag gaagccgaac ggctccacca
    25921 cccgccgcac ggcactggtg gttcctttgt gctgatgaat ataaaaagca tccttcacca
    25981 cctggcgttt gacgctttct gtccagctct cgtcccagcg ttccacagag aacgcccagg
    26041 cgagataagg caggaaactg accggacagg tcgcaggatt ccacaagtca cgcagtggca
    26101 cctgcagatc agaaatcccg ctgcaggttc gcgccagtcg gcgctccagt ggtgttgaac
    26161 ccggtggcag cagactattc atccgttcct ccgttggtta cgctccactg cgtacatgat
    26221 gccgcctgtg ttttgttcag gaccacatcc gccagcggcg aagccagctc cacacgctgc
    26281 accccctcaa catgcagggc ggcaaagatg gcgctacggc gaatatcccg accaagccgc
    26341 gtctgactgg cgatgtactt ctgcaggctg gcttttgccg cggccattac cggctctgct
    26401 tccggtcccg gataaagaaa aatggtggct tccacgcgat acgggatgat ttctgcgctg
    26461 cgaaccgtca gacggtcagc caccgggcgg acgttctcac tgttcagggc gttctccacc
    26521 acatccagca agtctttttc tgctgttccg tcgccttcgc ggctaaggac agtcagcacc
    26581 acctctgcag gtgccggact ggttgcactg gcatccgcca cccgaccgtc ggcgcttcgt
    26641 gcatgaaact cataagcggc agttggcccc gcaacagaaa gcccctcaaa ggctgcaggc
    26701 acacgcaggc gtaacgcttc atcgctttcc atcacagctg caacgggcgg cacagcgtca
    26761 ttatcagcag gcgtcaccgt caggcgtttc acgttgtagt tggcagcgag ctggtcaaga
    26821 tcgccgccca tcgcgtaagc caccatcaca gcctgcgcgg cttcgttaat gcgctggcgc
    26881 agaagcaact cacggtaagc gttctcctga agcaatttgg tgacgggttc agattccagt
    26941 tccagcgtgc ggatcacggc ttcctgctca tctttcggat gaagcgcaac aaattcggcc
    27001 ttgcgttcgg caagcagcgc ctcaaagtcc ggcacatcga caatctgcgg cgcaggcaac
    27061 tgcgaaaggt caatcactgc cattctctgc tcctgttgat acggaaaggg acacaggcac
    27121 accgttattc cgccgcccgg tcagctccac caccatagaa ccgtcaaagt tgctgttgat
    27181 ggtgatggaa tccagcgtca gccgtggctc ccagcgactc agcgccacat acactgccga
    27241 catgacctgc aggcgtaacg ccggattttg tggctggtct atcagtgccg acagcaggga
    27301 accatattca cgacgagcaa tgcggctacc ctgcggcgtc agcagaatgt cccgcaccga
    27361 ctggcgcaga tggtcaatat cagtaatgac tttgccgctg gtattgttca tcccgctata
    27421 aagcgtcata ccggtcctcc ggttgtatcg ccgcctttca ggacgccagt atgctgatgc
    27481 gcatcaacca cgatcccgtt agaactcatc gctccgccgc cctgggtaac accaccattg
    27541 atcaccactt cgctgttaat gcgcgtgcgg tcagcctcca gtacaaactc actggttttc
    27601 atggtgatgt tgtcagcggc ctcaatgacc attgatttga tgcccctgac ataccagcgc
    27661 ccggtggcgg gttcgtattc aaaccagcca ccgtcaggat gttctgtcac gcaggcgtct
    27721 gccgacgtcg acggtggtgc gaactgattc gaatagacag cgggcagcgc aaaggcagtt
    27781 tccagattgc cgcccaaact cagtagcacc acctgctcac cttccgatgg tttccaccat
    27841 gtgcgggcat tacccgcgcg cagcgtcagc cagttaatcc agttggtttc aaggtcgccc
    27901 gttttcaccc gacaaagcca gtgttcccgg tccacttcgg tgactacacc agtgcggatc
    27961 agattggtga taaggcgcat gatttcggtt aattgtgcgt tcatagggaa aggttgccat
    28021 caggggaaga aaggcggcag tgctgcaact tgtatcactg ctgatacaaa aatcaccccg
    28081 ccaaccattg cagaatcatg tcgcgggtca ttgcctcaac atcatcattt acgcccagaa
    28141 ggcgacgctc tgcgtaacgg acctccggtc ctttgcggct gacgcgatca cgcaggccat
    28201 aatggtgaac gcgggcaatg cgctgcacct taccttcaaa ctgcacgctg gcagaatcct
    28261 cgctggcggc ggttttcagg tattttgtgg tgcgaagttt tgtaaacatc tgccgtttga
    28321 tacggccttt tttactgcgt gctgttactc gcctcggttc atagctgctg ccatcaggat
    28381 tgcgttgcat cctgatgttc tgctgctgtg tccggcgcag ttcctgcgcc agctggcgca
    28441 tcatgcggct tctggcggct ggttccagat tcgccagcaa ggcactcagc cagtcgtcca
    28501 ctttctgcag ttcagccacg tttcaccgtc cacatttctt caggttcatc aggttccgct
    28561 atagcttcaa cgctcgacac actaccgtca gtgctgacca gcacacgctc cgtcagttgc
    28621 aggttcaggc tgatatcaca gacatcgttg cgcagaatat ccacctcaaa ggtgaatagt
    28681 ttttcccgta acgccgggtt attgatggca tcgggctggt tatccctcag ccacagcaaa
    28741 accggggcca tcagcagatt ctggtcgccg ctgaaatcct caatcaccgc gttcagggtg
    28801 taacggtact cccacgacat ggagctggcc cccgtggcaa ccagcgaacc gttatccaca
    28861 aacagatgca gtttgtccgg gttattgcgg acataaggca ccgctttatt gagggcgtgg
    28921 cgcagggatt gtggtttgtt cactgtttcg ctcctgacac gcaataatca tatccacttt
    28981 gtctgcacag accgcccagg cggcctccgt ttcatccagc aacgcattca gatcaccgtt
    29041 agtgcgcggc gctgcctgat ccagccgaca cggcgtcact cgcggacaac cactgacggt
    29101 aagctgcacc tccggtgagt gtggggcgtt cccgcagccg gataatgtca gcaggcaaag
    29161 gagtatcagc ccagcggcgt aaatcctcgt tctcacgttt cagttcctcg atccggtgtt
    29221 gtcgttgtct cagcagcgcg ctggtctgtt ctgcttcggc atagagccgc gcctgctccc
    29281 ggttattggt ttcagtcaga atagacaggc tgataagctg gctgttgctc tttgccagtg
    29341 cctggctttt gctctgcagc tcgtctgcct gcgtgctgat ggtctggctg gcatcagcca
    29401 gccgccacgt ctgccagccc agcgccgcca gtaataacgc cagcacaacc agcagtaacc
    29461 ggttcatgct gctacctgtt gcgccatctg attacgggtg atccagaagg caataacggt
    29521 cagtagataa aagaccaggg taatagccca ccccgtccag gcgagactta caacaatcag
    29581 caatcgcatc acccaactgg taaatacgtt ttcttttcgg gtaattgtct tcagcaaaga
    29641 tgccctcaac tcctgccaga gcgggccatt cttaattaac gcaaccagtg ctaccggaat
    29701 taccgcccat gtcagcaaac aggctaccca aacgccggac gctgccagta ccggaaaaat
    29761 cccctgcgga tacaccattg ctgcgattaa cagcgccatc cataacatca gaaacagtcc
    29821 gctgattaat ttctttttca tttcagtttg ctccctgtaa acaccaggcc atctcccgcg
    29881 cacggcggtt atccagcccc tgattaaaca caccttttac ataaacccag cgcggcaact
    29941 gtcggcacgc atccgcccag cgccgctgat tgagcaattt caccagcgtg gagctgcagg
    30001 cattgcccgt ccccacgttg aaggcaaacg acaccgtagc gtcatacacc ttctgtggtg
    30061 gctgttgctt cacacacctt tccagtgccc gctccacacg cagcacgttg gagatcagcc
    30121 cttctgctgc ctgtcgttcc gtgattgttt tgccgggaat gacgcccgat gtattaccaa
    30181 tgccgtcggt ccagacaccc gcgctgcact gatacggctg cagacgacag ccttcgtaat
    30241 cggcaatcag tttcagcccc tccacggagg tgtgaagctg ctgaaaaccc ggcagcgtgg
    30301 cagcaatagc cagcacggcc ccgacaaggc agcgtttaac gattgatgga ttcatagtcc
    30361 tcccgcgaga tctgcccgtc gcgcagaagc tggtaggctt tgtgtttgta gtaccagttg
    30421 atagccagca tcagcacacc aatcatcagg ccgcccagcg ttgaggcatc cttgatggac
    30481 aaatcgccca gccaggccag cacgacggcg atgcaatacg tgataaaggc gctgattcgc
    30541 tcaagcgtca taattcagtc ccatagctgg acggtctgca cggtggtggt ggtcggaatg
    30601 tccggcagct ccacctgcag cccgtgaggt aaaaaggggc cgtattcggc aagccccgga
    30661 tttgccttca gtacctgctc cgtgacaccc tgcgtgcgcc cgtaatgacg ccagcaaagc
    30721 gcgtccaccg tgtcatactg atgcgcacgc actttcatca gataagctcc actgtgcagt
    30781 gcggcgcatc ctgcacccgg ctgatggccc agcgggcgtc acgccacaaa tcaccgcttg
    30841 cttccgccag ttcctcgccc cgcttcacac cggacgccgt ggcgtcatag tcctggtaac
    30901 gttcgttgag catggcgcgt gcccagcagt aaaccgcgtt gaaatagtgc tgaatgcgct
    30961 cacttttgcc gtccagctgt tccgccggaa cctctgccag cgaggcatac cccagcatct
    31021 gctggcgtct gcgaaactca tacagctctg cgttgacctc cgaaattgcc gatagcgcaa
    31081 cctgctttaa acgcggctgc gtcaccgtgc cgtcagtgcg catcacgctg cgaaactccg
    31141 acaggtccac atcaggccag aacggcgtat tcctgatgat ttccgcctgt tccggtgcct
    31201 gttctggcgc aacaaacttc atgctgcttt ctcctgaaat agagggcggt ggacggggtt
    31261 ttgatgtggc agtgcctttc gccaccccgt gccgcccgtg cgcgggggca cgttctgtca
    31321 gcggctgtca ttgcgcagtc tgcgctccag ctgctgtttg tcttttttca cgccacagcg
    31381 gggatcgagc tgtaacgcat ggttgagatg attaagggcg gacgccggat tgctttcact
    31441 caggacagcg ccaatcgctt tatgcagacg cgcccgtgac tggtccggca tatccagacc
    31501 gtctgtcagt tccagcgtct gcagtaatag atccgcatca aagccggtgg cggcaagcat
    31561 tgcgctctgc gccgcgtctg acatttcctc tgccagcacg gtctgcacgt tgcggttacc
    31621 cagcggcatc acccagccat gacgcagagc atgacgcccg atctccagcg ccccggcata
    31681 atctccggca tcaatgcgcc acagcatcac gtacatcagc acgtcatcct gttgagcgcc
    31741 tccggcagcc aggacgccct ccgcccaggc ggcatatttc ggcagtagct ccaccttgat
    31801 ttccgctttt ttcaccgtgg actgaacgcc cttgagacgg cggcggtctt ccgccagttg
    31861 cagcagcatc aggtcatagc ccgatgcgtg gcgaacactg ccgccctcgc gggcggcctg
    31921 ttcagcctga acgcgcaggc gatgctgccg tgcgggactc aggctcatgg attacgctcc
    31981 ggtttcggct gcggcggcgc tgaaatcacc aatctggatg ttttccacca gtgcggcaca
    32041 gcggtagtcc tcaaccacat aggcttcgtt aacggattca aagttttcaa tccggtcacg
    32101 tttcgggttg tcgataactg aacggcggcg ggtgtcttcc tgccagtaga tggacaggtt
    32161 atccagacgg gtgatcagca gcgcattcgg cgggaagaac ggcgcacgca cggcctgcag
    32221 gccacccatg cgtttctgac tgatgatcat atcggcagcc agtttttcac tgttttcctg
    32281 ctctttgttg accagcggga aatacttgtc agacagcagt tcacgaccgc aaatcaccac
    32341 cagatcgtca tcgtcctggt agaccacgtc gataagctca ttgacggcat ccatcaccac
    32401 ggcgtccagg ttggcatatt cgccaccttt cccgactttc actgcgcccg gtgtggtttc
    32461 accgcccgtg gtggtgctgc ccatgacgtg atccggtgca tcctcacgga ttttctgcag
    32521 ccagccttta ttcacatcct gcagtagcgg gttttcgcta cggttagagg ttttcgcacg
    32581 cttcacgccg ttaaagccga tcatgatgcg gtccagtgcc tgacgtttca cgatggcgtt
    32641 acggatacgc acctggaaat cctgaaactt cgcccacagg tccagcttcg cgtaggtcag
    32701 caccgtgtca aagttggtct gttcgcattt gtattccaca tcgaccatca gcgtcggatc
    32761 gacaggttca cgctctttcg cggtggtgtc agtggttccg gcaatggtgc tgccaacacc
    32821 cagccccagc agctgaccgg actgctcagt cactggcgtg acgttaatca gcgtcaggaa
    32881 agcggcggac tgctggatct ggtcttccag cgtctgctgc acagacggct ccacagtgaa
    32941 cttgctggac agttcttcaa ctgccacacc gttcagacgc gccagctgct gcaggtaagc
    33001 gttaaaagca aagcgggtat tcttcttcat cgggttttat gctccatcag caattggtca
    33061 gagtgtcagc aggggcgtta ccgcctgttg cacgctggcg gtagtcctgg cggcagtcct
    33121 catggctcag cttgtccacc agttcgttaa aggcggcctg ttgtgcctgc agggcagtct
    33181 ccaacttaga caggcgttct tcctgctcag acagggattt ttcggtgcgt gcgctcaggt
    33241 tttgctgctc agtggcgacc agctccacgg ccttatgcac atcagagaac cgggcatcgt
    33301 cggactgctc ttttttggtg aacagcgccg tgacgcgggc aaacagggac ggcttgtcct
    33361 cctggatttc ttccagttcg atcaccgttt cctctgcggc ggtaaagaga ttggcgggat
    33421 tctgcttgcg gtttgccagc gggttatggg ctgcactggc gctgaatgtc agcatttccg
    33481 tacccagact ggctggatca tcagtggcgg ccaggccaac caggtaggct ttgcccgtat
    33541 cagcgaactt cgggctgact tccatagagg tgaataattt ctggcctttt ttcaccagtt
    33601 ccaccaggga ctccgttggc tcaacgtcgg catacagtgc catcttgccc gccagcgggc
    33661 cttccgtgat ttcttcagca aacagcgccg tcaccttgcc gtagcggtta aaggtgctgt
    33721 ccggcagata agacttgatg tgctcaaggt taatcagcgc ggtgtacacc gccgggttgt
    33781 agctggctgc catctgttcc agccattcac gctggatttc gcgtccgtcg gtggtggcac
    33841 cttccacccc gatgcgaaaa cgctttgctt tcactgtcat gagccgtgct ccgttagaaa
    33901 aaacttactg gagccttatg gttgcggtga tgggggcagt gaaacaatgc gcggtatttg
    33961 taccgactac cacacaaacc gcaggcgggg aaagccttca ttcaaggctg taggtttgtg
    34021 ccatgaacac cacactgaca cccgcagatc tcgatccccg tcggcaggcc atgctgctgt
    34081 actttcaggg ataccgtgta gcccgcattg ctgaaatgct gggcgagaaa gttgcaaccg
    34141 ttcacagctg gaagaaacgc gacaagtggg gtgactatgg gccgctggat cagatgcagc
    34201 tcaccaccgc cgcacgctac tgccagctca ttatgaagga gcacaaagaa gggaaagatt
    34261 tcaaagagat tgacctgctg gcgcgccagt cggaacgcca cgcgcggatc ggcaagttta
    34321 acaatggcgg caacgaagcc gacttaaacc ctaacgtcgc caaccgcaac aaaggcccgc
    34381 gccgtcagcc ggaaaagaat gttttcaccg atgagcagat tgagaagctg gaagaaatct
    34441 tccattcctc catgttcaac taccagcgcc actggtggga agccggaaaa accaaccgca
    34501 tccgcaacct gctgaagtca cgccagatcg gcgcgacctt ttactttgcc cgtgaagccc
    34561 tgattgacgc tctgcttacc ggacgtaacc agattttcct ttccgccagc aaggcacagg
    34621 cccacgtctt taagcagtac atcatcgact tcgccaaaga aatggaagtg gagctgaaag
    34681 gcgatccgat ggtgcttccc aacggggcca cactgtattt cctcggcacc aatgcccgca
    34741 cggcccagag ttaccacggc aacctgtatc tggatgaata tttctggata ccgaaattcc
    34801 aggagctgcg caaagtggct tccggtatgg ctattcacaa aaaatggcga caaacctatt
    34861 tttccacgcc atccagcctg acacacagtg cttatccgtt ctggtccggt gcgctattca
    34921 accgtgggcg caataaagcc gacaaggtgg acatcgacct gtcccacagc aatctggctc
    34981 caggcctgct gtgtgcagac gggcaatacc gccagatagt caccgtggaa gatgcggtgc
    35041 gcggcggctg taacctgttc gaccttgacc agttgcgcat ggaatacagc ccggacgaat
    35101 accagaacct gctgatgtgt gaattcgtgg acgatctcgc gtccgtattt ccgctcagcg
    35161 agctgcaggc atgcatggtg gacagttggg aagtctggac cgactttcat gcactggccc
    35221 tgcgcccgtt tggctggcga gaagtgtgga tcggttatga cccggcaaaa ggtacgcaga
    35281 acggcgacag cgccggatgc gtggtggtgg caccgccagc cgtgccaggc ggtaagtttc
    35341 gcattcttga gcgtcaccag tggcgcggga tggacttccg cgcccaggct gacgccatca
    35401 aaaaactgac cgaacagtac aacgtgacct atatcggtat cgactcgaca ggtgtcggtc
    35461 acggagttta cgagaacgtg aaagcgttct ttcctgccgt ccgggagttt gtctacaacc
    35521 ccaacgttaa aaatgccctg gtactcaagg cctacgacat tatcagccac cgccgtctgg
    35581 agtttgacgc cgggcacacc gacattgcgc agtcattcat ggcaatccgt cgtgccacca
    35641 ccgccagtgg caaccgcccg acctatgaag ccagccgcag cgaagaagcc agccacgccg
    35701 atctggcctg ggcaacaatg cacgcactgt ttaacgaacc gctgcagggc gagtccgcca
    35761 ataccagcaa tattgtggag attttttgat gggaaagagt aagaaaaacc gcgctgcggc
    35821 gacgaatcag atccagcata aaaaccagac ttcagccgaa gcattcagct tcggtgatcc
    35881 cgttccagta cttgaccgcc gcgaactgct ggactatgtg gaatgtgttc agacagatcg
    35941 ctggtatgag cctcccgtca gctttgacgg actggcgcgc accttccgcg ctgccgtgca
    36001 tcacagttcc ccgattgcag taaagtgcaa cattctgacc agtacctata tccctcatcc
    36061 gctgctcagc caacaggctt tttcgcgttt tgtgcaggac tatctggtat ttggtaacgc
    36121 ctacctggag aaacgcacga accgattcgg tgaagttatc gcccttgagc ctgcgctggc
    36181 aaaatacacc cgacgcgggt tagacctgga tacctactgg tttgtgcaat acggtatgac
    36241 aacccagccg tatcagttca cgaaaggcag catttttcat ctgatggaac cggacatcaa
    36301 ccaggagatc tacggcctgc ccggctatct ttctgccatt ccatccgccc tgctcaacga
    36361 atccgccacg ctgttccgcc gtaagtatta catcaacggt agtcatgcag gcttcatcat
    36421 gtacatgacc gacgccgcgc agaatcagga ggatgtgaac aacctccgca acgcgatgaa
    36481 aagcgccaaa ggtccaggca acttccgcaa tctgtttatg tactcgccta acggcaaaaa
    36541 ggacggactt cagattatcc cgttgtcaga agtcgcagcg aaggatgaat ttcttaacat
    36601 caagaacgtg agtcgggatg acatgatggc agcgcaccgc gtgccgccgc aaatgatggg
    36661 gattatgccg aataatgttg gggggtttgg ggatgtcgaa aaggcgagtc atgtatttgt
    36721 tcgtaacgag ttaattccat tacaaaaaag gctaattgaa ttaaatgaat ggcttaatga
    36781 cgaaattatt cgttttgacg aatataaatt aggcaatact tgattaaagc ctgtttaaac
    36841 tggctgtcgg aacagccagt ttaaacaccg gcaccaatga aatcgccata tgaatcttct
    36901 tcaaggctga tgtattctac cctccattga tccatccgcc tcaatgcata cagggagaaa
    36961 tgagtatcag caacagctat gcacgtattc aattctataa gttctgaatg ccccaaggtt
    37021 ggatcaccat aaaacttata aaacaaagcc tttagatcag aacatgtttg ttcagccgct
    37081 cttctggcat cggggccacc ttcagttgca tcataaacaa cattgataga aagctcatat
    37141 ggtatgcctt cctcaagatc gttaaatctt tcttcgccca aatcgaaaaa aactccgatc
    37201 aaatgctctg cgtttgtggc gataatatcc gcaacttctt tttcgaaaat aaacttttta
    37261 tcctcgtcgt aagcccgcaa gcgctcttca aacgagttag gaaacgctgg acgaccatac
    37321 ttagcagcta accattgctt aagagcttgt ttctcttctg tgctaatgga gaatgctgga
    37381 tcgttttcct cacaacagaa agcttcttta ggaatgatga ctttcctttc atgccggagc
    37441 tctactgcat tcttcttcgc actttctggg ttttcaaaac atagatcgag tattctcgga
    37501 tgcttagctc ttttcatcct gcttgacccc tttttcaagg ggccaaacat cagttccacg
    37561 tttttttctg aattactctg taaatcacaa tcatgcgtta taactacaac tttatggttc
    37621 gtttcatcag gctgcagaat tccaagtgaa acggcatcat catgtttaag tacatgcccc
    37681 tgacgccatg atgttcctcg taccaacata aatgtgattt attcctctac cgttccagga
    37741 attgaaatag atgacttcca atcatctgtc ggtttcgcct tgcttgctaa gtaattggcc
    37801 gattcagcag cagcattcat cgctttagct tcatcaatca atacctgaac tgccctattc
    37861 caatcctcac cttcttttac tacgtccatt aatgacttac cattaaatgc tttcattttc
    37921 accaatagct tcgcatcatg gagtccagct ttggaaaaag agtcagcgac attactaagg
    37981 ttggtaatga attgcacttt tgaagcatca tcaggctggt tctcccctga caaccattta
    38041 tagagagcct gccgagttat ccccaaatcc tttgccagtt ctgacattga tggagacagc
    38101 acttctcgaa tattagctaa atgttgggca acattcctga catcaacctc aggagaaatt
    38161 gcaggcgtgt acgttgattc tatggaatca aatgcaaact gaacccttgg ctgaacatac
    38221 tgacgccatt gcttataagc atttacaggg tatgaagccc ccgtaccaac taatagtaaa
    38281 gatgcggcga cagcaacaac acctgataca catgagcttg actgcaaatt tggagaatat
    38341 cgatctacag agcctatgct tccggtggga attgcataca ttttagtcac ctccgttgtt
    38401 tcagtgccat ttagtacggg caaaatctga aaccatgcct tcaaatgctt ctttaacttt
    38461 actatgcaac gaaagaatct gtttttcaat taactgcaaa tcagttgaca tgttgccttc
    38521 aacataatga tctgtatcaa ttattgcgtg catacgatgc tctgtgttac taaatctcgg
    38581 caacggcagt aaaccattag gaatcatatc tggcggaaaa cctagctgac cattcatctt
    38641 atgtattcgt gaaaccataa atccattagg gatcaatggc tcaacacatg tctgatatac
    38701 ggactcttgt atagactgta ttggcgtcca tccgaaatca acaccatgaa gttcctttac
    38761 aagatactgc tcaattgttt cgcttttctc aggaaacaca gcatccaaat atcgaagccc
    38821 aatccgactt aacagcgatg gcttagcaaa ttctagaact ttgctcagac caagtatcaa
    38881 tgacgagatg aaagatacat ggttatcata atatgttgta tggaacgtaa tgaaatcgtt
    38941 gcctaaaaca aaaccagatc ttctatctgc atcaatcatc aaccaacttg tcacaggctc
    39001 aaatgaatgt accggtggct cattggggtt ttttatttca aatttcaact gagttgtatt
    39061 ggaaacctca aataaaggaa atccctcaac tcttagcgca tcttgtatgt ctggaacata
    39121 cttactcatt gcagctattg gagtaaactt tacctgtacc aatgcatagt aaacgggcgc
    39181 attggacata cgttcgctgt tagtactcat gccgccctcc ttgaaattgt ttcatctcat
    39241 atctctttat cttactaact gtagtttaca cataggttga cactttctcc atcattaagt
    39301 tcactatgac atcaatagcc aaaactctta cgtaatcgct tcggcgcgcg ctcgtatccc
    39361 cgccacgcct gcccgcttta tgtagtggtt ttcatgcacc tgcatgatct acgcaaaagc
    39421 ccaccagttc tggcgggcct tagcaaaaac gatcctcaaa cgatcatgcg atctcatgcg
    39481 gcatagacat gcactacaga gctaacgcct cgcaagggct cgttgttcaa ccttgctgac
    39541 gccagaaaca agttcagacg ccagcaacgt ttcttaatgc agccagctgt cgtcttccca
    39601 caccttctgc ataattttca tcacttgctt cctttcttcg tccagttgca gtccggtcag
    39661 ttccacaccg ttagagctac ctttgcggat acgaattacc gttttgggat acagggggcg
    39721 cagattacgg taaagctcgg attcaagggc gtccagggta gactggctaa tcttctgctc
    39781 tttatcgatc attatttcaa tgcgcataaa agtcacctca gctgatgaca tccattgagc
    39841 ggttgtattc gtggcttctg atttttgcca tgagttcatc agtcaattca gaaacccact
    39901 gcagagccag tcccttctct tcatcactac actcactggc cgctacaagc ttaagaaaaa
    39961 aatcaatgcg ctggagcttc aaagactcca aaaaatagtc ctgcatcttt cctcctatga
    40021 caccacacgc aatactgtat gtataaccac tgtttataat tacagtatat aataatctta
    40081 ctgatgtaaa acgttttttt acgtttatca gcctgatatg cctggtatta ttaagagcac
    40141 gaatggttaa ccatcgtaat taatacaggt ttcgccactt atcatcttcc tgcaaacgct
    40201 ggttccgata gaagatacgc aggcctgctc ctgacgaaat actgccgccg cgaaggagta
    40261 aatcgacctc tttctcgcta ccatcaaacc ctctggactt cagctcatac acgagctgct
    40321 gtcgctgatg gtctgtaatt cgctgtttgt agtctttaca ccgtttcggt ttaaccaggt
    40381 gtaaccttgc tgccagttcc cggcgttctt ttttgctcat actgtgcagg taatcgtgca
    40441 actccttgtc atccatgcgg gtgatatccg ttctggtatc cccatcagct gatttgtctt
    40501 tcccttgttg gttcaaattt tcagcaaggg gacagttatt gccacgagtc caaggggcgc
    40561 aagcgccctg gtcggctgcc gcctcctgaa cgtcaacggc cttacgaacc attttccact
    40621 tcacggcatg agtgcagatt ttgccctctg caatgggtga ccagatgcca taaatacgaa
    40681 tgccgtgatc gccataggcg gtcggctctt cgttgatttc ataagcggtt ctgatgaggt
    40741 gatatttacg gggaaccagt acgccgccct gcttcatgat gtaggtggca aaacaaccag
    40801 catcagctgc agccagaatg gcatcaaggc gcgggttatc cagtaccggc gcacctgctt
    40861 ttttgtcacc ctgttgcctt gccgcctgac cagccagcaa tcgcagttca cggtaagcct
    40921 gacgccccgg aatgccaaag aagcggaatt gctgaacacg atgcagagac gcccaggcat
    40981 taacgtattc agcgttatca cgcagagatt tacccgtttc cttgctgatc tcgccagcca
    41041 gaccacgccc gtcaatgttc ttactgatgt atttcgcgat gtagctagtc ggcgttcctt
    41101 tgcgcgggtt tatcagctca gacttaaagc gtggccccgt gttattaccc agctcctcgc
    41161 ggtcttcacg gatagcaaac ttacgcaaca atgcagtaat ggcgcggcgg tcttttttgc
    41221 gcatgaaaca caacaggtgc cagtgaactg tgccgtcatg atgcggctca gccacccgca
    41281 cgccatacca gcgcaacccg gctttgtgca tcgccttacg aaatgcagca aacatgccga
    41341 ccagataatc actgctttgt cttaccgtcg catttgtcca ggtcgggttg ggcctaccgt
    41401 tatttagcgt ggaatggaaa cgtgacggac aggtgatggt gtagaaaacg gcgcagtcac
    41461 cgcgcatttc cgcgataagc tccagacctt taacacaggc catcatctca ttgcggcgat
    41521 gcgcagggtt gctgctgctg gcgtttacca catcctccat gtccagcgtg tcgccgtctt
    41581 cgttcaccag ttcatgagaa cggaaaaact ccagcgactt acggcgctgc tcacgtttat
    41641 gcatcacggc ttcatagctg acatagggag atgctttttt gctgaccagg caaacagcac
    41701 gcaactgctc ttcccaccat tcgcaacgca tcttccacaa tttccgatac caccagtcgg
    41761 cgcacagcat acgcgccagc gaccccggaa tgagttcata gggcacgggt ttacggcggt
    41821 ttcttttccg gcggagttgc tcaaacgcag gcggtatgac atccagtcgc agggtttccg
    41881 ccgccacctt ttcccatgtc ttgcggattt cttctggctt aacgtcatcg gtggcataca
    41941 aatcgccaca agcggcatca aggcacatgc tcatatgcgc agctaccagg gtggacaggc
    42001 gtttcacctg atcctgactc atttcaggca ggatcagcag accgtccagc ccttcatggc
    42061 ttgccataaa gcgaaaagaa gtggatagct gactgtcgcg tacatgctcc agtcgttcca
    42121 gacatggctt aatcgtctca cgcaaatagc gggaataagc ctttggcctg cccaggctgc
    42181 tgaagtattc aatacgttgc atcagcggct tgctgatatg ggaaggctgg gcgttaacgt
    42241 ccgccagaat gaccatgccc ggattaaaac gctgctgctc atgcgccagc tttgcccggc
    42301 taatgagctt atcctgctcc atttcgcgct ggacaggatc acgtgattca ttaaagaaat
    42361 aacgctccca tacctgatca ctcagagcct cgcggcgcag ttgttcctgc tcgttatcgg
    42421 tagcgtacag agtgatcagg tttgaaagcg cagaaaccgg cgcaacttcc gccgggtcca
    42481 gataagggtt aatggccttt ttcgggctgt tccatgagaa tgctgcggcg acctcgttaa
    42541 agccgctgca gttgttcata tcagcatggc tcatgcacgc actccgtaca cggcagaact
    42601 atccacgcca cgcgaaggat caaatcccac ccagcagcgc ggcccggaaa cagcgatgat
    42661 ttctgttgca gatttactct caccagctgc tacaccgatg ctgcgttttg ccttgatgta
    42721 gtggtgagta aaattgcgat acagcgaacg gatcagggat gtatcactgt tagaaacaat
    42781 gaccggatgt ccttctgatg accgatgttc aagaacggat gccaggtgat actggtcatc
    42841 ttcagtgaaa ccatcagtgt gatagccgga aaacgtaccg tcatacggcg gatcgcaata
    42901 caccacatcc cccacctgca gcatcgccag cgtttcatca aagcttgcgc agataaacgt
    42961 tgctcgctgg gctttttctg caaatgtgcg aagttctttt tcagggaaat acggattttt
    43021 ataattaccg tagggaatgt tgaaatgccc gctcttgtta tagcgacata aaccacggta
    43081 accgtgacga ttgagataca ggaaatatac cgctttcatg aaatcagtaa tttcagtgga
    43141 gtaattaaac tcctgcctta tgttgtaata agccacctcc ctgtttgcga tctcaaataa
    43201 aactctggcg cgagatataa acgattcaca atcagcggca acctttttat agaggttgat
    43261 taaatcagga ttaatatccg caaccagata gctggggtaa tccgtctcca tcatcacagc
    43321 acaagaaccc gcgaaaggtt caaccagtcg cgggcctgca ggaaggtgtt ttttcagttc
    43381 ggacataatg gcggttttat tacccgccca tttcaggatg gtgctcatac agcacctcct
    43441 gcaataacat atcctaaagc ttctaatggg gttaatgagc gaattgatag catcacccat
    43501 tgttctgaaa ctgccatgac gtcattaacc ggaagcacat gagagataac cgcggcccat
    43561 tccctacccg taaatacgcc atgcttccat tcgcaaagag aaagaacatc accaacttta
    43621 tagccacgat cgtctttacg aagttcagcc gtcttttgac ctgcaaccac agcgttgaaa
    43681 tacttaggtg caatttttaa ttgatggata cgcacagccc ctgtcataca gcacctccgt
    43741 tgtaatgttt gcctttcagc tctgcgattt cctggcaagt aatgcaaagc tgcacacccg
    43801 gaatggcgcg gcgtcgtgct ggcggaattg gcgcttcaca ctcaatgcaa agcacgcgag
    43861 acacgcccgg tgttttggca cgagcagcac ggatatggct ctggcgttct tcttcaacgc
    43921 gctgctgtac gagatccatt gcatcagcca ttagtggatc tcctgcgctt cgttctggat
    43981 tgcttcagca gtcacacgca gcagttctgc cgcttccacg tggtttagct gacgggatga
    44041 gatatgacac gccaggctat caaggcgagc tgccattgct tcagcccttg cccggcgttc
    44101 ttccagacga gcctctgtca gtaaaatatt aagccctgca tcatccggtc cggttttagt
    44161 cgtaaggatt tcaatattac gcataatcaa ttctcctgaa tttagataaa gggatgcccg
    44221 gcgggtttac gccattaatt tcattagttg gttaattcgg catggttagc cgtttgggaa
    44281 ataagctcac cactgcacga aaatgattca ttgctttaat caactcccgc ttttcgtcag
    44341 tggtcagctc attaatgctg atgctatgac gttcagctgg aatttttgcc ataaagaata
    44401 tggcagccag tgctcgttta ttttgttcgt tattgatatc ccgtggatca cgcatatctt
    44461 taataaaccg ctcaagctct gactcaatat tcaggccaaa tactttcgcc cttaactccg
    44521 caatgtgatt aagtccattc aggcgttcac cggggcttaa tggaacagtc gccgcagcgc
    44581 cattaattgc cataattcat atccccaaaa cgcaactatc gttctttgtt cttacggtaa
    44641 cgttcaagag gagatacatt ttttcgtatc gtctctttaa cctgctctcc ccgtaaaaac
    44701 gtcccatccc ttagcgtgaa aaagtaactg ccatcgcccg acaacgacgg ataacaacag
    44761 agcaaatcat cttcaggtac tgaataactc tcccctctgt aacgaaactg ataaaccact
    44821 tcactttccg ctgcatacat tttgactttc tccgtttcct cgtggtcaat tcagacagca
    44881 attcatcttg tgaatgacat ggatgccagc gtttaccatc ctcacccatg atccagccgt
    44941 gaccgtagtg cattgccggg ctttgtttta ccagcagcga tgcaaatgat ggttctttcg
    45001 tcagcataag cacctcacag caaaccgaat gaagcaccga ggccagtcac ggtatcaact
    45061 gcactcacca tcgcaggatt agcctgtaaa cgggcctgca atgaaacagc cgccagcgcc
    45121 atcagtcgtg ttacagagtt aatgctgctg atagcatcac gacgaccggc actagttttt
    45181 acatcaccag ataccgcacc tgctgcaaca cgcccgatct ctgcagttgc gctcatgacg
    45241 taatgcggca gtttctcttt tgccacctca ttaatcggta cacatggcag acaatgaatc
    45301 tgtgccagaa aaccatctac cagcgttgaa tcttcagtca gatcggtaag cagccagatt
    45361 tctggtgcgg ttaataaatg aggctgagct gggttcagct tgttccgcag aatctgcaca
    45421 ttcatgcctg cacgttctgc cagttgcacc aggttgtgac gcagtgcaaa agccctacag
    45481 gcttcatcga aatgcggatg tttggaaatc ttgtaatcaa acatggtgcc cccttagaaa
    45541 gttcccataa ttgaacttac ttaccaacaa tgacgcggaa gttggaatga ccgagggatt
    45601 cacggacctg atcagttttg tacatcagat aacgaaggct tacgcggcct ttattttttt
    45661 ctttcttgac catgtactta gcaagttgac catggtgaat tttttggtaa acagagccac
    45721 gggaaatgcc ctcccactct gcgaactctg caggcgtagc catctctttt ggtacacgaa
    45781 ttgaaatatc agtactcata gtgcagtatc tcttactttg tgtgcgtgtt agttcgtttt
    45841 agctcgtctc tttaactctc acatcaagag acacgaagac attacgatct tgattcaaga
    45901 ttgtcaaatg gagattacca atgttaaaca tcagaatggg ttccgatacg ggaggtaagg
    45961 cagctattga gaggctgctt gaggcttatg gattcacaac taagcaggca ttaagcgagc
    46021 acctgaatgt ctcaaaaagt actatggcaa acagagtgtt acgtgacagt tttcctgctg
    46081 actggataat tcagtgtgca ctagaaaccg gtgtttcgtt actttggtta gccactggac
    46141 agggaagcat gaaagaagga tacgagcctg agaaaagatc tcataacgag aacaaacaag
    46201 caattaaacc gttatccaaa ctcataactc catctattcc taaaggaacc ttggaaaatg
    46261 gacaactcag tattgatgaa gagattttcc tagaccacag catattacct gcagattatg
    46321 aagaatcgat gttcttagaa acccctactg attgttatct catcgataaa tcagttaaac
    46381 aagtcagcaa tggattctgg cttatcaata ttgatggaat gattattatt gcaaaaatca
    46441 tgcggattcc cggcaataag attgtagtaa atcaagatga agcgtctttc gagtgctctg
    46501 ctgatgatgt ggaagttatt gggcgtgcag tcaaagtaat aaagagtatc taaacatgac
    46561 tgtcagaaaa cagccaaacg gtaaatggtt gtgcgagtgc tatcccaatg gacgcaatgg
    46621 caagcgcgtg cgtaagcaat ttgctacgaa aggcgaagcc attgcttttg aaagcttcac
    46681 aatggaagaa gtgaacaaaa aaccatggct gggggaaaag gaagatcggc gacacctatc
    46741 agaattaatt gagcagtggt attccctgta tggtcaaaca ctcgcagacc ccaagcgcct
    46801 catagcgaaa cttagaatta tctgtaatgg tctaggcgat cccatcgctt cagaactgac
    46861 agccggtgac tttacgaaat accgcgaagc acggttaaaa ggtgaagtac gaaatgaaga
    46921 tggcacgttt atgtcgcccg ttaagccccg cacggtaaac cttgaacagc gcaatctatc
    46981 atctgttttt ggtacactga aaaagctggg ccactggtca gcccccaacc cgcttgctgg
    47041 gctgccaaca tttaaaattg ctgagagtga attggcgttc ctgaccccgg aagaaattaa
    47101 acgtctgctg gatgcctgcg ccgattctca aagctccagc ctgcttttga ttgcaaaaat
    47161 atgcctggcc accggcgcac gctggagcga agccgaaaac ctgcagagtc atcaggtatc
    47221 aaaataccgt atcacttata ccaagaccaa aggcaagaaa aaccgaactg tgcctatttc
    47281 taaggattta tatgaagaac tgcctaagaa tagggggaag ttattcaccc catgcagaaa
    47341 agcctttgag cgtgcagtaa agcgggctgg tatcgatctg ccagagggtc aatgtactca
    47401 cgttctgcgt catacattcg ctagccattt tatgatgaat ggtggaaaca tattggtttt
    47461 gcgggatatc ctaggacatt cagatataaa aatgaccatg gtctatgcac acttcgcccc
    47521 agaccattta gaagatgccg taactaaaaa tcctttattt aatttgaggt aagtagtaat
    47581 ttatgaatag cgaactagag ttacttataa aaatatt
//
LOCUS       DAZFVO020000017        47227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFVO020000017 DAZFVO020000000
VERSION     DAZFVO020000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 47227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.017"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(99..386)
                     /gene="yebO"
                     /locus_tag="ACHKNB_RS04520"
                     /old_locus_tag="pgaptmp_000904"
     CDS             complement(99..386)
                     /gene="yebO"
                     /locus_tag="ACHKNB_RS04520"
                     /old_locus_tag="pgaptmp_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HGU4194469.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(461..604)
                     /gene="mgrB"
                     /locus_tag="ACHKNB_RS04525"
                     /old_locus_tag="pgaptmp_000905"
     CDS             complement(461..604)
                     /gene="mgrB"
                     /locus_tag="ACHKNB_RS04525"
                     /old_locus_tag="pgaptmp_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HGU4194470.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            763..1002
                     /gene="yobH"
                     /locus_tag="ACHKNB_RS04530"
                     /old_locus_tag="pgaptmp_000906"
     CDS             763..1002
                     /gene="yobH"
                     /locus_tag="ACHKNB_RS04530"
                     /old_locus_tag="pgaptmp_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HGU4194471.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1145..1936)
                     /gene="kdgR"
                     /locus_tag="ACHKNB_RS04535"
                     /old_locus_tag="pgaptmp_000907"
     CDS             complement(1145..1936)
                     /gene="kdgR"
                     /locus_tag="ACHKNB_RS04535"
                     /old_locus_tag="pgaptmp_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HGU4194472.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2113..3486
                     /gene="yebQ"
                     /locus_tag="ACHKNB_RS04540"
                     /old_locus_tag="pgaptmp_000908"
     CDS             2113..3486
                     /gene="yebQ"
                     /locus_tag="ACHKNB_RS04540"
                     /old_locus_tag="pgaptmp_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194473.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3532..4413)
                     /gene="htpX"
                     /locus_tag="ACHKNB_RS04545"
                     /old_locus_tag="pgaptmp_000909"
     CDS             complement(3532..4413)
                     /gene="htpX"
                     /locus_tag="ACHKNB_RS04545"
                     /old_locus_tag="pgaptmp_000909"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HGU4194474.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4605..6653)
                     /gene="prc"
                     /locus_tag="ACHKNB_RS04550"
                     /old_locus_tag="pgaptmp_000910"
     CDS             complement(4605..6653)
                     /gene="prc"
                     /locus_tag="ACHKNB_RS04550"
                     /old_locus_tag="pgaptmp_000910"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HGU4194475.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6673..7371)
                     /gene="proQ"
                     /locus_tag="ACHKNB_RS04555"
                     /old_locus_tag="pgaptmp_000911"
     CDS             complement(6673..7371)
                     /gene="proQ"
                     /locus_tag="ACHKNB_RS04555"
                     /old_locus_tag="pgaptmp_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HGU4194476.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7468..7965)
                     /gene="msrC"
                     /locus_tag="ACHKNB_RS04560"
                     /old_locus_tag="pgaptmp_000912"
     CDS             complement(7468..7965)
                     /gene="msrC"
                     /locus_tag="ACHKNB_RS04560"
                     /old_locus_tag="pgaptmp_000912"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HGU4194477.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8095..9378
                     /gene="yebS"
                     /locus_tag="ACHKNB_RS04565"
                     /old_locus_tag="pgaptmp_000913"
     CDS             8095..9378
                     /gene="yebS"
                     /locus_tag="ACHKNB_RS04565"
                     /old_locus_tag="pgaptmp_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HGU4194478.1"
                     /translation="MALNTPQITPTKKITVRSIGEEQPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9347..11980
                     /gene="yebT"
                     /locus_tag="ACHKNB_RS04570"
                     /old_locus_tag="pgaptmp_000914"
     CDS             9347..11980
                     /gene="yebT"
                     /locus_tag="ACHKNB_RS04570"
                     /old_locus_tag="pgaptmp_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HGU4194479.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12060..13499
                     /gene="rsmF"
                     /locus_tag="ACHKNB_RS04575"
                     /old_locus_tag="pgaptmp_000915"
     CDS             12060..13499
                     /gene="rsmF"
                     /locus_tag="ACHKNB_RS04575"
                     /old_locus_tag="pgaptmp_000915"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HGU4194480.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13617..13853
                     /gene="yebV"
                     /locus_tag="ACHKNB_RS04580"
                     /old_locus_tag="pgaptmp_000916"
     CDS             13617..13853
                     /gene="yebV"
                     /locus_tag="ACHKNB_RS04580"
                     /old_locus_tag="pgaptmp_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HGU4194481.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13958..14149
                     /gene="yebW"
                     /locus_tag="ACHKNB_RS04585"
                     /old_locus_tag="pgaptmp_000917"
     CDS             13958..14149
                     /gene="yebW"
                     /locus_tag="ACHKNB_RS04585"
                     /old_locus_tag="pgaptmp_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HGU4194482.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14150..14806)
                     /gene="pphA"
                     /locus_tag="ACHKNB_RS04590"
                     /old_locus_tag="pgaptmp_000918"
     CDS             complement(14150..14806)
                     /gene="pphA"
                     /locus_tag="ACHKNB_RS04590"
                     /old_locus_tag="pgaptmp_000918"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HGU4194483.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15202..15543)
                     /gene="yebY"
                     /locus_tag="ACHKNB_RS04595"
                     /old_locus_tag="pgaptmp_000919"
     CDS             complement(15202..15543)
                     /gene="yebY"
                     /locus_tag="ACHKNB_RS04595"
                     /old_locus_tag="pgaptmp_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HGU4194484.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15556..16428)
                     /gene="copD"
                     /locus_tag="ACHKNB_RS04600"
                     /old_locus_tag="pgaptmp_000920"
     CDS             complement(15556..16428)
                     /gene="copD"
                     /locus_tag="ACHKNB_RS04600"
                     /old_locus_tag="pgaptmp_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HGU4194485.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16432..16806)
                     /gene="yobA"
                     /locus_tag="ACHKNB_RS04605"
                     /old_locus_tag="pgaptmp_000921"
     CDS             complement(16432..16806)
                     /gene="yobA"
                     /locus_tag="ACHKNB_RS04605"
                     /old_locus_tag="pgaptmp_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HGU4194486.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16945..17175
                     /gene="holE"
                     /locus_tag="ACHKNB_RS04610"
                     /old_locus_tag="pgaptmp_000922"
     CDS             16945..17175
                     /gene="holE"
                     /locus_tag="ACHKNB_RS04610"
                     /old_locus_tag="pgaptmp_000922"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HGU4194487.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17277..17933
                     /gene="yobB"
                     /locus_tag="ACHKNB_RS04615"
                     /old_locus_tag="pgaptmp_000923"
     CDS             17277..17933
                     /gene="yobB"
                     /locus_tag="ACHKNB_RS04615"
                     /old_locus_tag="pgaptmp_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HGU4194488.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17957..18619
                     /gene="exoX"
                     /locus_tag="ACHKNB_RS04620"
                     /old_locus_tag="pgaptmp_000924"
     CDS             17957..18619
                     /gene="exoX"
                     /locus_tag="ACHKNB_RS04620"
                     /old_locus_tag="pgaptmp_000924"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HGU4194489.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18616..20676)
                     /gene="ptrB"
                     /locus_tag="ACHKNB_RS04625"
                     /old_locus_tag="pgaptmp_000925"
     CDS             complement(18616..20676)
                     /gene="ptrB"
                     /locus_tag="ACHKNB_RS04625"
                     /old_locus_tag="pgaptmp_000925"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HGU4194490.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASHDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEGGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20885..21544)
                     /gene="yebE"
                     /locus_tag="ACHKNB_RS04630"
                     /old_locus_tag="pgaptmp_000926"
     CDS             complement(20885..21544)
                     /gene="yebE"
                     /locus_tag="ACHKNB_RS04630"
                     /old_locus_tag="pgaptmp_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HGU4194491.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21871..22227)
                     /gene="yebF"
                     /locus_tag="ACHKNB_RS04635"
                     /old_locus_tag="pgaptmp_000927"
     CDS             complement(21871..22227)
                     /gene="yebF"
                     /locus_tag="ACHKNB_RS04635"
                     /old_locus_tag="pgaptmp_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HGU4194492.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22294..22584)
                     /gene="yebG"
                     /locus_tag="ACHKNB_RS04640"
                     /old_locus_tag="pgaptmp_000928"
     CDS             complement(22294..22584)
                     /gene="yebG"
                     /locus_tag="ACHKNB_RS04640"
                     /old_locus_tag="pgaptmp_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HGU4194493.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22718..23896
                     /gene="purT"
                     /locus_tag="ACHKNB_RS04645"
                     /old_locus_tag="pgaptmp_000929"
     CDS             22718..23896
                     /gene="purT"
                     /locus_tag="ACHKNB_RS04645"
                     /old_locus_tag="pgaptmp_000929"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HGU4194494.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23952..24593)
                     /gene="kdgA"
                     /locus_tag="ACHKNB_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /gene_synonym="eda"
     CDS             complement(23952..24593)
                     /gene="kdgA"
                     /locus_tag="ACHKNB_RS04650"
                     /old_locus_tag="pgaptmp_000930"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4194495.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24630..26441)
                     /gene="edd"
                     /locus_tag="ACHKNB_RS04655"
                     /old_locus_tag="pgaptmp_000931"
     CDS             complement(24630..26441)
                     /gene="edd"
                     /locus_tag="ACHKNB_RS04655"
                     /old_locus_tag="pgaptmp_000931"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HGU4194496.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26676..28151)
                     /gene="zwf"
                     /locus_tag="ACHKNB_RS04660"
                     /old_locus_tag="pgaptmp_000932"
     CDS             complement(26676..28151)
                     /gene="zwf"
                     /locus_tag="ACHKNB_RS04660"
                     /old_locus_tag="pgaptmp_000932"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGU4194497.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28489..29358
                     /gene="hexR"
                     /locus_tag="ACHKNB_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /gene_synonym="yebK"
     CDS             28489..29358
                     /gene="hexR"
                     /locus_tag="ACHKNB_RS04665"
                     /old_locus_tag="pgaptmp_000933"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HGU4194498.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29486..30928
                     /gene="pyk"
                     /locus_tag="ACHKNB_RS04670"
                     /old_locus_tag="pgaptmp_000934"
     CDS             29486..30928
                     /gene="pyk"
                     /locus_tag="ACHKNB_RS04670"
                     /old_locus_tag="pgaptmp_000934"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGU4194499.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVMEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="ACHKNB_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /gene_synonym="msbB"
     CDS             complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="ACHKNB_RS04675"
                     /old_locus_tag="pgaptmp_000935"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HGU4194500.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="ACHKNB_RS04680"
                     /old_locus_tag="pgaptmp_000936"
     CDS             complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="ACHKNB_RS04680"
                     /old_locus_tag="pgaptmp_000936"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HGU4194501.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="ACHKNB_RS04685"
                     /old_locus_tag="pgaptmp_000937"
     CDS             complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="ACHKNB_RS04685"
                     /old_locus_tag="pgaptmp_000937"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HGU4194502.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34499..35254
                     /gene="znuC"
                     /locus_tag="ACHKNB_RS04690"
                     /old_locus_tag="pgaptmp_000938"
     CDS             34499..35254
                     /gene="znuC"
                     /locus_tag="ACHKNB_RS04690"
                     /old_locus_tag="pgaptmp_000938"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HGU4194503.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35251..36036
                     /gene="znuB"
                     /locus_tag="ACHKNB_RS04695"
                     /old_locus_tag="pgaptmp_000939"
     CDS             35251..36036
                     /gene="znuB"
                     /locus_tag="ACHKNB_RS04695"
                     /old_locus_tag="pgaptmp_000939"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HGU4194504.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36183..37193)
                     /gene="ruvB"
                     /locus_tag="ACHKNB_RS04700"
                     /old_locus_tag="pgaptmp_000940"
     CDS             complement(36183..37193)
                     /gene="ruvB"
                     /locus_tag="ACHKNB_RS04700"
                     /old_locus_tag="pgaptmp_000940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGU4194505.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37202..37813)
                     /gene="ruvA"
                     /locus_tag="ACHKNB_RS04705"
                     /old_locus_tag="pgaptmp_000941"
     CDS             complement(37202..37813)
                     /gene="ruvA"
                     /locus_tag="ACHKNB_RS04705"
                     /old_locus_tag="pgaptmp_000941"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGU4194506.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37952..38017)
                     /gene="yobI"
                     /locus_tag="ACHKNB_RS04710"
                     /old_locus_tag="pgaptmp_000942"
     CDS             complement(37952..38017)
                     /gene="yobI"
                     /locus_tag="ACHKNB_RS04710"
                     /old_locus_tag="pgaptmp_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HGU4194507.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            38088..38690
                     /gene="yebB"
                     /locus_tag="ACHKNB_RS04715"
                     /old_locus_tag="pgaptmp_000943"
     CDS             38088..38690
                     /gene="yebB"
                     /locus_tag="ACHKNB_RS04715"
                     /old_locus_tag="pgaptmp_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HGU4194508.1"
                     /translation="MNINYPAEYEIGYIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38692..39213)
                     /gene="ruvC"
                     /locus_tag="ACHKNB_RS04720"
                     /old_locus_tag="pgaptmp_000944"
     CDS             complement(38692..39213)
                     /gene="ruvC"
                     /locus_tag="ACHKNB_RS04720"
                     /old_locus_tag="pgaptmp_000944"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HGU4194509.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39248..39988)
                     /gene="yebC"
                     /locus_tag="ACHKNB_RS04725"
                     /old_locus_tag="pgaptmp_000945"
     CDS             complement(39248..39988)
                     /gene="yebC"
                     /locus_tag="ACHKNB_RS04725"
                     /old_locus_tag="pgaptmp_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4194510.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40017..40469)
                     /gene="nudB"
                     /locus_tag="ACHKNB_RS04730"
                     /old_locus_tag="pgaptmp_000946"
     CDS             complement(40017..40469)
                     /gene="nudB"
                     /locus_tag="ACHKNB_RS04730"
                     /old_locus_tag="pgaptmp_000946"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HGU4194511.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40587..42359)
                     /gene="aspS"
                     /locus_tag="ACHKNB_RS04735"
                     /old_locus_tag="pgaptmp_000947"
     CDS             complement(40587..42359)
                     /gene="aspS"
                     /locus_tag="ACHKNB_RS04735"
                     /old_locus_tag="pgaptmp_000947"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGU4194512.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42669..43235
                     /gene="yecD"
                     /locus_tag="ACHKNB_RS04740"
                     /old_locus_tag="pgaptmp_000948"
     CDS             42669..43235
                     /gene="yecD"
                     /locus_tag="ACHKNB_RS04740"
                     /old_locus_tag="pgaptmp_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4194513.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43232..44050
                     /gene="yecE"
                     /locus_tag="ACHKNB_RS04745"
                     /old_locus_tag="pgaptmp_000949"
     CDS             43232..44050
                     /gene="yecE"
                     /locus_tag="ACHKNB_RS04745"
                     /old_locus_tag="pgaptmp_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HGU4194514.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44103..44498
                     /gene="yecN"
                     /locus_tag="ACHKNB_RS04750"
                     /old_locus_tag="pgaptmp_000950"
     CDS             44103..44498
                     /gene="yecN"
                     /locus_tag="ACHKNB_RS04750"
                     /old_locus_tag="pgaptmp_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HGU4194515.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44539..45282
                     /gene="cmoA"
                     /locus_tag="ACHKNB_RS04755"
                     /old_locus_tag="pgaptmp_000951"
     CDS             44539..45282
                     /gene="cmoA"
                     /locus_tag="ACHKNB_RS04755"
                     /old_locus_tag="pgaptmp_000951"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HGU4194516.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLDPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45279..46250
                     /gene="cmoB"
                     /locus_tag="ACHKNB_RS04760"
                     /old_locus_tag="pgaptmp_000952"
     CDS             45279..46250
                     /gene="cmoB"
                     /locus_tag="ACHKNB_RS04760"
                     /old_locus_tag="pgaptmp_000952"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HGU4194517.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(46415..>47227)
                     /gene="torZ"
                     /locus_tag="ACHKNB_RS04765"
                     /old_locus_tag="pgaptmp_000953"
     CDS             complement(46415..>47227)
                     /gene="torZ"
                     /locus_tag="ACHKNB_RS04765"
                     /old_locus_tag="pgaptmp_000953"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4194518.1"
                     /translation="NDFDVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARA
                     QRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVKNALGTPSGKIEIYSKTL
                     EKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAYPAHRLHSQLNYAELRKKYAVADR
                     EPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENG
                     LCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDQPAIQA"
BASE COUNT        11224 a        12247 c        11656 g        12100 t
ORIGIN      
        1 agagttttaa tttgaagcag ttgcaccatg gcagtgcgta taatcaggat gaattgagta
       61 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccagacga
      121 ataatatctt cgtcttcaac cgatttttga ctctcaacgg tctttttatc tgctttctca
      181 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      241 aacgcctcaa gcagttcaat ctgttcgtta gtccgagaac tggcacgatt gataaaaaac
      301 cacaagatga gcccgataag aagaaccacc accgatacaa ccaaagacgc aatgttcatc
      361 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      421 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      481 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      541 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      601 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      661 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      721 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      781 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
      841 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
      901 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
      961 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1021 ttctcactca tgagtaaaat attcttatag caagcgcgtt taatataacg caccttcaac
     1081 tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca ggttcttact
     1141 ctcatcagaa cggatagtcg tgataaccca tttgggcaga aattttgcgc gctgcggtat
     1201 gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga
     1261 tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac cggtaccgca atgcatcgca
     1321 gcccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc tggtccagaa
     1381 cgggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta tactccacgc
     1441 cctcaagaat ttgcttcact tcatcgagat cgcgccatgc cagcagtacc ttaccaatcg
     1501 cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag
     1561 agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg tggatagttt
     1621 ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt aaatcgacgt
     1681 tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat ttctccgact
     1741 ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga taaacggtgc
     1801 tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc tcttcaccca
     1861 gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc tgtttatcca
     1921 gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca gaactgtttt
     1981 ttattataat ttcgcaccag ggtggtcgca atccatcttt tgccggttag ttacaattct
     2041 gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat tcaccaaaga
     2101 tataaaattc ctatgccaaa agttcaggcc gacggcctgc cattgcccca gcgatacggt
     2161 gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc aatcgccaac
     2221 gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc catctgggta
     2281 gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt tctgggcgat
     2341 atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt gtcttcactg
     2401 ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc
     2461 ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt
     2521 tttctgggga gagggatggg cataaactcg tttattgttg ccgtctcttc tgctgccggg
     2581 ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac
     2641 gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct
     2701 cgcgccagta aaccccgttt cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc
     2761 ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt aattgctgcg
     2821 gaactggtgg taatggttgt tgttggtatt ttctttattc gccgccagct ttctcttccc
     2881 gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca
     2941 tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt ttacctgcaa
     3001 accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca
     3061 acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc aggattgctg
     3121 ggtgctctag ggttgttcat catggctgcg gggctttttt ccctggttct gctgcccgcg
     3181 tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg atttggcttg
     3241 ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga
     3301 gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc ggcactggtg
     3361 gcgctgatgc taaatcagtt cggtgataat ggtacgcacg tttcgctgat ggctgcggct
     3421 attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc acgatccagg
     3481 gcataaaaaa agcgcgtcga tcaggacgcg ctttttagta tttacttcat attacttcag
     3541 gtattcaccc gtacgcagag cttcaattcg tttatccagc ggcgggtgag tcatgaacaa
     3601 ctcactgagc gatttcgact taccgttaat gcagaaagcc atcatgctgg ttgcttcttg
     3661 cggttcatag ctggttttca ggcgctgcag cgcggcaatc attttctcgc gaccaaccag
     3721 ttttgccgaa ccggcatcag catggaattc acgatgacgc gagaaccaca tggtgataat
     3781 gctcgccaga ataccaaaca ccagttccag aaccgttgca accgcaaagt agatcagcgg
     3841 gttgccgttg ctctcttcac cttcatcacg atttccgccc ataaaacccg cggcaagctg
     3901 cgccagaata cgggaaataa agataacgaa ggtgttcacc acgccctgaa tcagcgtcat
     3961 ggtgaccata tcaccattgg cgatgtggct gatttcgtga gcaattaccg cctcggcttc
     4021 atccgggctc atgttctgca gcaaaccggt gctgacagca accagagagg catcacggcg
     4081 cgcaccggtt gcaaaagcgt tgatgtccgg cgcatggtag atagccactt gcggcatagc
     4141 gatccctgcc tgacgagcct gggttgctac agtattaacc agccaacgtt ccctttcgtt
     4201 acgcggttgc tcgatcactt ccccgccaac agatcgtaat gccatccatt tggacatcag
     4261 aagcgaaacg aaggaaccac caaaaccgaa cagcaaggcc atgatcatca gcccctgaac
     4321 gctgctcgac tgtatccctg tcaggctcag taccagcccg aaaacgacca ttacggccag
     4381 gttcgttagc aggaagagcg cgattcgcat cataattttc tttttacctc agtttaacaa
     4441 aacgcaatat gcgataccca catcgtatgg gttacgcgac tattttcaag tctggatagt
     4501 gcgtaagtca ccagaaagac acaactttac attttgtagc atctgattta cggcatcttg
     4561 ccgctgttaa aaaatcaggc acaatttctt gtgcctgatt gatattactt gacgggagcg
     4621 ggttgttccg cgggtctggc tttttcaagc ttcgccagat cgagtgcgat attcaccgtc
     4681 tcatccagat aaggatccgg ctcctggtaa tctttcggta gatcatccag cttcttcaac
     4741 tccggtttac cttcgcgttt aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct
     4801 tcattattct ctttctcacg cacagcgtag ttcagagaaa cgatattgcg cttgtccttc
     4861 atagcgttga agcgcgcgat atccttcatg atgttctgga actcaggatc tttcgcgata
     4921 cgcgcattat gttccttcag cagctccggc ccaaaggccg ttaaatcgcc tgatttcaca
     4981 taagtcgcgg catcaatgct atcccacggc agcgcgttat cttcgaattt ctcacccgtt
     5041 tccgtttctt cattacccgt cggcatgatg atgtctggcg ttacgccttt acgttgcgta
     5101 ctgccgccgt taacgcgata gaatttctgg atcgtgtact gcacagaacc cagcgctggc
     5161 cattcaggac gtaacatctg atcgtaaata cggttcaatg aacggtattg ctgaacggtg
     5221 cctttaccaa acgtcggttc acccacaacc agcgcacgac cgtaatcctg cattgccgcg
     5281 gcaaagattt ctgaagccga agcactgaaa cggtcaacca gtaccaccag cgggccttta
     5341 tagaacacct gcccgtcggt atcgctgtct tcacgaacct tgccgttgtt atcgcggacc
     5401 tgaacaatgg gacccgcagg aataaacaga cctgagagcg ataccgcttc ggtcaacgcc
     5461 ccaccgccat tgctacgcag gtcgatgatc acactgctga cattctgttt ttccagtttc
     5521 tgcagttgaa ctttgacatc gtctgtcaaa cccacataga agcccggaat atccagcacg
     5581 ccgactttct ctttaccgac ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga
     5641 atacgttcac gggtcaatgt tacagtgcgg gtcttggtcc ctttaccagc aggtaaaatt
     5701 tccagacgaa ctttactgcc cttcggccct ttaattaagg caaccacatc atcaagacgc
     5761 cagccaatca cgtcaaccat cggcttgcct gtttgaccaa caccgacaat tttgtcacca
     5821 acgctgatag ctttactctt cgctgccgga ccacctgcca ccatcgaatt gataacggtg
     5881 tagtcatcat ccatttgcag cactgcgcca ataccttcca gcgacaaact catttcagtg
     5941 ttgaactgtt cggtattacg cggggaaaga tagttggtat gcgggtcgat ttcacgcgca
     6001 aacgccgtca ttgccagcga gaaaacatct tcgctgttgg tttgcgccag acgacgaatg
     6061 gcaaatttgt agcggcgtgt cagggtttca cgaatttctt tatccgtttt tcctgccagc
     6121 ttcaggctta actcgtcgaa tttgacttta ctgtcccaca gcgcgttcaa ctcagcctcg
     6181 tttttcggcc agggcgcttt gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc
     6241 atcggctttt ccagtaccga caaagcgtac tggtaacgct caaaacggcg cttttgcgcc
     6301 agattgtaga gatcgtagaa aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg
     6361 gttttctttt tcgcgaactg ttcaacatcg cttgccagca gcacgttgtg gctgtaatcg
     6421 agcagattca ggtagcggtc aaagattttg gccgaaaatg cctgatcgag gtcgaactgg
     6481 cgataatgag aacgggtgaa gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc
     6541 tcttccttta ataccggaat ttgatcagca cgcgtgatat cttctacagc gaaggtctgg
     6601 cctgctattg caagcaggcc agctaacgcg gtaagcctaa aaaacatgtt catgcctggc
     6661 ccggcctccg tttcagaaca ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt
     6721 cagctggacg cggacgccgt ctttggtgat ttctaatacg gtggcatcca tcgcgttttg
     6781 acccgctttc accttcaggg cttgtccgac agtcagagct gaaatgtcag aaaccggggt
     6841 gtgctgttct tcgcgaggtg cttttactgt ttttggcgct ttctctaccg gcttttgcgc
     6901 acgaggttta cgttcagcgc cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc
     6961 gcggcgcggt gcgtcttctt tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg
     7021 ctgttcagca cgctgtgcct gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg
     7081 ctctacatgt tgctcgtcca gctcaccgca tgggttgccg tcaagatcga cacgcgttgc
     7141 gccgggttta acaccgtaaa gataacgcca gctcgaagtg tagagacgta aagcggatcg
     7201 taattgcgtt ttgctcaggt tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat
     7261 accgattttc agcggacgcg cttcaccttc cgcactgaaa cagtggggaa aacgttcggc
     7321 cagaaacgcg attacttctt tactgctatt caacttaggt tgattttcca tgaaatttcc
     7381 tgattacaac ggacgtagcc aacaagcgca ggcatgaaca ggcgtcatta taatgacgct
     7441 accagtaaat gctacgttat ccgttgatta tcctgcgacg ctcgcaaaga attttttgta
     7501 atccgtcgtt gcaagcactt tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc
     7561 ctcgtctgta aagcgaccga agacggtact atcgatgtcg agaacaccaa taatctgatt
     7621 tttcaccacc agcggcagaa caatttcaga attactcgcc gcatcacagg caatatgccc
     7681 gtcaaacgcg tgaacatcct cgatacgctg cacttgattg cgggcaaccg cagtgccgca
     7741 caccccgcgc ccgacgggta tccggacaca ggcaattttg ccctgaaatg gtccgagtac
     7801 cagtgtatcg tcctcaagca aataaaaacc tgcccagttt acgtcagtga gacgctcgta
     7861 taacaacgca ctggtgttcg caagcgttgc cagaaaactg gtttctcctg ccatcagcgc
     7921 gttaaagtcg cgatttaaat ccgcgtaaaa ttctgttttg ttcattatat aatcacttgg
     7981 ttgtcttacc tggaactgcc agcctattaa aataagcatt aaatgcgtta atgctcaaga
     8041 tcattcccat catgggttaa tattaatgtt aattcttatt acatttggca cgtcatggct
     8101 cttaacacac cacaaattac gccgacaaaa aagataacag tgaggtcaat cggcgaggaa
     8161 cagccgcgtg gtgattacca acgttgcccg caatgcgaca tgctgtttag cctgcccgag
     8221 ataaattctc atcagagtgc ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc
     8281 gactggtcgc taacgcgcct ggcggcaatg gctttcacca tgctgttgct gatgccgttt
     8341 gcctggggcg aaccgctgtt gcatatctgg ctgttaggta ttcgtatcga cgccaacgtc
     8401 atgcaaggca tctggcaaat gaccaaacag ggcgatacga taacgggggc gatggtcttt
     8461 ttctgcgtta ttggtgcccc cctcattctg gtgtcctcca tagcatattt atggtttggt
     8521 aaccgactgg gaatgaattt acgtccggta ctgctgatgc ttgagcgact taaagagtgg
     8581 gtaatgctgg atatctacct ggtcggcatt ggcgttgctt ctataaaggt acaggattat
     8641 gcccatatcc aggcgggtgt cggcttgttc tcttttgtgg cgttggtgat tttaacgacg
     8701 gtgacgttgt cacatcttaa tgtcgaggag ctgtgggagc gattttatcc gcagcgcccc
     8761 gctacgcgta gggacgagaa acttcgtgtc tgtcttgggt gccattttac cggctatcct
     8821 gatcagcgtg gtcgctgccc gcgttgccat atcccgctac gcctgcgtcg ccgtcatagt
     8881 ttgcaaaaat gctgggcggc gctgttagcg tcaatcgttt tgttgttacc tgccaacctg
     8941 ttgcctattt ctatcattta tctgaacgga ggacggcagg aagatacaat tctttccgga
     9001 attatgtcgc tggcaagtag caacattgcg gttgcgggaa tcgtgtttat cgccagtatt
     9061 ctggtaccgt ttactaaagt gatcgtcatg ttcactttac tgttgagcat tcattttaaa
     9121 tgccagcaag gtttacgcac acgcattctg ttactgcgga tggtgacctg gattggtcgc
     9181 tggtcgatgc tcgacctgtt tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag
     9241 atcctcgctt ttactatggg accggctgcg ttttatttcg gcgcagcggt aattttgact
     9301 attcttgctg tggaatggct ggacagccgc ttactttggg atgcacatga gtcaggaaac
     9361 gcccgcttcg acgactgaag cgcagattaa aaataaacgc cgtatctcac ctttctggct
     9421 gctgccgttc atcgcgctaa tgattgccgg ttggctgatt tgggacagtt atcaggaccg
     9481 gggtaatacc gtcaccatcg actttatgtc ggcggatggt attgtcccgg gccgtacgcc
     9541 tgttcgttat cagggcgttg aagtcggaac agtgcaggat atcagcctca gcgacgatct
     9601 tcgtaagatt gaagtcaagg tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga
     9661 gactcaattc tggctggtga cgccaaaagc atcgttggca ggtgtctccg ggctggacgc
     9721 cctcgtcggt gggaactata tcggcatgat gccgggtaaa ggtaaagagc aggatcactt
     9781 tgtcgcactc gatacccaac cgaaatatcg gctggacaat ggcgatctga tgatccacct
     9841 gcaagccccc gatctcggtt cgctgaacag cggttcattg gtctatttcc gcaagatccc
     9901 ggtgggaaaa gtctacgact atgccatcaa tcccaacaaa caaggcgtgg tgattgatgt
     9961 cctgatcgag cggcgtttta ccgatctggt gaaaaaaggt agccgtttct ggaacgtttc
    10021 cggcgttgat gccaatgtca gtatcagtgg cgcgaaggtg aaactggaaa gcctggcggc
    10081 actggttaac ggtgcgattg ccttcgattc accagaagag tcgaaacctg ccgaggcgga
    10141 agataccttt ggtctgtatg aagacctcgc ccatagccag cgtggcgtaa taataaaact
    10201 ggaactgccg agtggggccg gattaaccgc cgactcgacg ccattaatgt atcagggact
    10261 ggaagtcgga cagctaacta aactggattt aaatcctggt ggtaaagtca ctggggaaat
    10321 gacagttgat cccagcgtcg ttaccctgct tcgtgaaaat acccgcatcg aattacgtaa
    10381 tccgaaatta tcccttagcg atgccaatct cagcgccctg ctgactggca aaaccttcga
    10441 gttggtaccc ggcgatggcg agccacgcaa agagttcgtt gttgtgccag gcgaaaaagc
    10501 actgctgcat gaacctgatg ttctgacgct gaccctgacc gcgccggaaa gttacggtat
    10561 tgatgcgggt cagccgctca ttcttcacgg cgtgcaggta ggccaggtta tcgatcgtaa
    10621 actcaccagc aaaggcgtca cctttaccgt cgccatcgag cctcagcatc gagaactggt
    10681 aaaaggcgat agcaaatttg tcgtcaacag ccgtgtcgac gtgaaggtgg ggctggatgg
    10741 cgttgagttt ctcggtgcca gcgcctcaga atggattaac ggcgggatac gtattctgcc
    10801 gggcgataaa ggcgagatga aagccagcta tccactgtat gccaatctgg aaaaagcgct
    10861 ggagaacagc cttagcgatt tacccaccac aaccgtgagt ttgagtgcag agacgctgcc
    10921 ggatgtgcag gcaggatcgg tagtgctcta ccgtaaattt gaagttggtg aagttattac
    10981 cgtccgtccg cgagctaacg cgtttgatat cgatctgcat attaagccgg agtatcgcaa
    11041 ccttctgacc agcaatagcg tgttctgggc agaaggcggg gcgaaagttc agctgaatgg
    11101 tagtggcctg accgtacagg catccccgct ctccagagca ttaaagggag ccattagctt
    11161 cgataacctc agcggtgcca gcgccagtca gcgtaaaggc gacaaacgaa ttctgtatgc
    11221 ttccgaaaca gcggcccgtg cggttggtgg gcagattacg cttcacgctt tcgatgccgg
    11281 aaaactggcg gtcgggatgc caattcgcta tctcggtatt gatatcgggc aaatccagac
    11341 gctggatctg attaccgcgc gcaatgaagt acaggcaaag gcggtgctct atccggagta
    11401 tgtccagacc ttcgcccgcg gcggtacgcg cttctcggtg gtcacaccgc aaatttcagc
    11461 cgcgggcgtt gagcatctag atactatcct ccagccgtat atcaacgtcg aaccaggccg
    11521 gggcaatcct cgccgcgact ttgaattaca agaggccacc attactgatt cgcgttacct
    11581 ggatggctta agcattattg ttgaagcgcc ggaagccggt tcgttaggta ttggtacgcc
    11641 tgtgctgttc cgtggtctgg aagtcggtac ggttacagga atgacgctgg ggacattgtc
    11701 agatcgcgtg atgattgcga tgcgcatcag taaacgctat caacacctgg tgcgtaacaa
    11761 ttccgtcttc tggttggcat cgggttacag tctggacttt ggtctgacgg gcggcgtagt
    11821 gaaaaccggc acctttaacc agtttatccg tggcggcatc gccttcgcca cgcctccggg
    11881 tacgccactg gcaccgaaag cccaggaagg caaacacttc ctgttgcagg aaagtgaacc
    11941 gaaagagtgg cgtgaatggg gaactgcgct tcccaaataa tgcccactgc tccggcgtgc
    12001 ctgcgccgga gcgtttatgc taaactgcgc gcctgttttt ttgccagtgg tacatgctcg
    12061 tggcccaaca caccgtttat ttcccggacg cctttctgac acaaatgcgc gaagcgatgc
    12121 cttcgacgct ctcatttgat gattttcttg ccgcctgtca gcgcccgttg cgccgcagca
    12181 ttcgcgttaa tacgctgaaa atctccgttg ctgatttcct gcaattaacc gctccttatg
    12241 gctggacgct tacgccaatt ccgtggtgtg aagaaggttt ctggattgaa cgcgacaatg
    12301 aagatgcatt gccattgggt agtaccgccg agcatttaag cggcctgttt tatattcagg
    12361 aagccagttc aatgttgccc gttgccgcct tgtttgctga cgataatgca ccacagcggg
    12421 tgatggatgt cgcagctgcg ccaggctcca aaacgacgca aattgccgcg cggatgaata
    12481 acgaaggggc aatccttgcc aatgagtttt ccgccagtcg ggtaaaagtg ttacatgcca
    12541 atatcagccg ctgtggcatc agtaatgttg cgctcacaca ttttgatggc cgcgtgtttg
    12601 gtgcggcagt gccagaaatg ttcgatgcca ttttgctgga cgctccctgc tctggcgaag
    12661 gcgtggtgcg taaagatccc gatgcgctaa aaaactggtc accagaaagc aatcaggaaa
    12721 tcgcagctac acaacgggag cttatcgaca gcgcctttca tgcattacgt cctggtggta
    12781 cgctggttta ctcgacctgt accttaaacc aggaagaaaa cgaagccgtt tgcctgtggc
    12841 tgaaagagac ttaccccgac gcagtagagt ttttaccact tggcgatctc ttccctggtg
    12901 caaacaaagc gctgaccgaa gaaggctttt tgcatgtttt cccacaaatt tacgactgcg
    12961 aaggcttctt cgttgctcgt ctgcgtaaaa ctcaggcgat tcccgcctta cccgccccca
    13021 aatacaaagt gggcaatttc ccgttcagcc cggtgaaaga tcgcgaagct ggacaaattc
    13081 gtcaggcggc tgcaggtgtt ggcttaaact gggatgaaaa cctgcgcctc tggcagcgtg
    13141 acaaagaact gtggttgttc ccggtgggca ttgaagccct gatcggtaaa gtccgatttt
    13201 ctcggttggg gattaaactt gccgagacgc acaacaaagg ttatcgctgg cagcatgaag
    13261 cggttattgc tcttgcctca cccgataata tgaatgcatt cgagctgaca ccgcaggaag
    13321 cggaggagtg gtatcgcggg cgcgatgttt acccgcaagc cgcgccagtg gcggatgatg
    13381 tattggttac tttccagcat caaccgattg gtttagccaa acggattggt tcgcgattga
    13441 aaaacagcta tccgcgtgaa ctggtgcgcg atgggaaact ttttaccggt aacgcctgac
    13501 agcgcacaaa aaaagcgcac tttttgactg gcacattcgg ctgcctcaac taggctgaaa
    13561 aatggtgcga tcggactggt cgtaccacaa tcggcagcta aatggagagc acgaagatga
    13621 aaaccagtgt gcgcataggc gcttttgaaa tcgacgacgg cgaattacac ggtgaatcgc
    13681 caggtgatcg aacgttaacc attccttgta aatctgaccc cgatttatgt atgcaactgg
    13741 atgcctggga tgctgaaacc agtatccctg ccctgctaaa tggcgaacac tctgtccttt
    13801 accgtacccg ttacgatcaa caatctgatg cctggattat gcgtcttgcc tgatccaaaa
    13861 agaacccgtc ggcatggcgg gttatttgtc ctggttattc ccccgttgta aaatctctcc
    13921 taaacttaac ggtacggcac cacacttcgg ggatgaaatg ttcgcgctgg tactttttgt
    13981 ttgctacctg gatggcggtt gtgaagatat tgttgtggat gtctacaaca cggaacagca
    14041 gtgtctttat tctatgagcg atcaacggat ccgccatggc ggttgttttc cgattgagga
    14101 ttttatagat ggtttctggc gaccagcaca ggagtatggt gatttttaat tattgcaatt
    14161 gcacaagagt cagttcgccc ccaaagacag caccggtatc aatataatgc aggttgccaa
    14221 tatccacgcg atgtcgcaac ggtgtatgac caaaccagaa atgatcagca cctgtaattc
    14281 cctgcccttt ttggcgttca cctaatcgcg agcggctcca caagacctga tgcaaatcaa
    14341 cgtccttttg ccattcataa acatcatctg gataatcggc atgagcaata acatgtttgc
    14401 cggtacgact gtgtacttca agaataaagg gcaaatgctg acatttttcc agcgccgttt
    14461 tcgcttgttt ctgttgatta tctgccagcg caataaacca gtcgccgcca ttcatcaacc
    14521 acaaagacat ctgctgggat gccagcgcat ccatcgccat ctgttcatga ttgcctctta
    14581 ccgcacaaac ccaatgttgt tccagtaact gcagacaacg taaactttgc ggcccacgat
    14641 cgataacgtc tcctactgag ataagtaaat ctcgccacgg atcaaaacga caatgccata
    14701 atttgcggcg caactgctca agacaaccgt gtatatcgcc agaaagccag atatgtcgcc
    14761 attgatgacc cgcaattctc tgataaacgg gcgcaggctg tttcatcaat attttcctcc
    14821 cgcgctaaag atcacataat cttaacaaga atgttaaaaa acgctggact cagacagtag
    14881 agtgtgtgtt atggttgact ataaagtcag cgaaggaaat gcttctggct tttaacagat
    14941 aaaaagagac cgaacacgat tcctgtattc ggtccaggga aatggctctt gggagagagc
    15001 cgtgcgctaa aagttggcat taatgcaggc ttagttgcct tgccctttaa gaatagatga
    15061 cgacgccagg ttttccagtt tgcgtgcaaa atggtcaata aaaagcgtgg tggtcatcag
    15121 ctgaaatgtt aaaaaccgcc cgttctggtg aaagaactga ggcggttttt ttattggaaa
    15181 tcaaaaggct attttaggta attaacagag tttttcagct cgttctataa acggtgccag
    15241 actcattttt tcgccgggat tgttaggatc atcaatctga atcaccgaaa tgggttgggc
    15301 tttagtcttc ccactggcaa cttccttttg tgcgatatcg tttaaaggat actgcacgag
    15361 ggtacttggg ttaatgacat acaaagcatt acccggtctg caagtcagca tcacctcttc
    15421 gcgattaaac gcccatttgt ctttacccac ttcaaaacga ctgacggtaa tcacctgcgg
    15481 tgcagccagc gccgctgcag aactggtgag taacagaaac gtcagaatac tttttttcat
    15541 cattttcgta atccatcaaa aaggttcgag agtcgcaagc aggctgatga tcaccagcac
    15601 taccgcacct atcgcccatt cgagtttagt catccactca aaccagggtg ccgcacgatc
    15661 ttcatcttgc ctcatgcgcg gtacaagaac ataacgatta gccagcgcaa tgaccaccat
    15721 aatcatcacc aggatggctt tgagcaataa gagttggccc cagtaagtcg taagcgtggg
    15781 gggaaatccg gtaatcaaca atgcattaag cacgccgctt gccagtacgc cgatcaccgc
    15841 aaaatgcccg caccaggaaa aacgcatcag cgcctgaatg gcctggtgtc gccaacggcc
    15901 tttgatgagt tgcatacacc agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca
    15961 aatcaggtga atcgcgtgat tagtctgatg gattttcgcc gttacccctt cattcatcgt
    16021 cgcatgcccc actcccgcca gcagaataaa ttgcgcggta gtgagcataa acagcaaacg
    16081 tgacacattc cgcggttgca taagggcgac gatcaacgtt acgagggcga gaacaatttg
    16141 ccataaccag acgccaccaa actgcgtttg caaaacagcc tgccaaatgt ttggcgaaaa
    16201 tacatctgcc catcccgttc ccatcaaccc gccctgaacg gcgagcattg cagtggcact
    16261 gatcaaactc cagacggcag cgtgctgttg taaacgcaga aaacgcttcg ccagcaagcg
    16321 acgaatcgtt aagggggcca gccaggcacc gtacattgcg aagccaaaga ccagcatcag
    16381 cgaggtgaaa tggataaatc gcagcgcgat ccaggtaaac gccagcatga tttatttcac
    16441 gctaaaggtg tagtgtcctt tcgttttatg cccgtccacc gaaacaacat gccagtcaac
    16501 gatgtaggtt ccgggtttca gcgaatcggc cagtggaaca atcagttgct tttggtcctg
    16561 ctcatttcgc ttcgccggta atgttttaat attttcgttt tttggccccg tgatttttgc
    16621 accactgaat cctgtttcaa caccttccga gaagtttaaa gtgattgcct gcggtgcagc
    16681 tgtcacttgc gcgtttgccg caggatactg atgcgttaaa tgcgcatgtg cccagacgga
    16741 aggtgtcacc aacgaagtgg tcaggattgc cagcgcgtag cgaagggagc gtgcagttga
    16801 agtcatatta tctattcctt tttgtaataa cttttttaca gagcataacc ttgtctaatg
    16861 tctgagtcga ggatcatcaa ttccggcttg ccatcctggc tcactcttag taacttttgc
    16921 ccgcgaatga tgaggagatt aagaatgctg aagaatctgg ctaaactgga tcaaacagaa
    16981 atggataaag tgaatgtcga tttggcggcg gccggggtgg catttaaaga acgctacaat
    17041 atgccggtga tcgctgaagc ggttgaacgt gaacagcctg aacatttgcg cagctggttt
    17101 cgcgagcggc ttattgccca ccgtttggct tcggtcaatc tgtcacgttt accttacgag
    17161 cccaaactta aataaaactt atacagagtt acactttctt acataacgcc tgctaaatta
    17221 tgagtatttt ctaaaccgca cccataattt gcagtcattt tgaaaaggaa gtcattatgt
    17281 cgttctggaa agttgcagca gcgcaatatg agcctcgtaa aacctcactc accgaacagg
    17341 tagctcatca tctggagttt gtgagggccg ccgccaggca acaatgtcag ctgttagttt
    17401 ttccatcgct ttctttactg gggtgtgatt attcgcgacg tgcccttcct gccccacccg
    17461 atctctcact gttagacccg ctttgttatg ccgcaacgac ctggcgaatg accatcattg
    17521 ccggccttcc cgttgaatat aacgatcgct ttattcgtgg aattgcggtg ttcgcaccgt
    17581 ggcgaaaaac gcctgggatc taccatcaga gtcatggtgc atgtctgggt cgtcgttcca
    17641 gaaccatcac ggtggtcgat gaacaaccgc aaggtatgga tatggacccg acctgttcac
    17701 tgtttactac agggcaatgt ctgggagaac ccgacctgct ggcgtcggcc cgtcgcttac
    17761 aatttttttc acatcagtac tcgattgccg tgctgatggc aaatgcccgt ggtaacagtg
    17821 cactatggga tgaatatggt cgtctcatcg ttcgcgccga tcgtggttca ttattgttag
    17881 tcggtcagcg ttcatcacag ggttggcaag gcgatatcat tccattacgc taggcttttt
    17941 cggcctggag catgccatgt tgcgcattat cgatacagaa acctgcggtt tgcagggagg
    18001 gatcgttgag attgcctctg ttgatgtcat tgacggaaaa atcgtcaacc ccatgagcca
    18061 cctggtgcgc cccgatcgtc ctattagtcc acaagcgatg gcgattcatc gcatcaccga
    18121 agccatggtc gccgataaac cgtggattga agatgtgatc ccacactatt acggtagtga
    18181 atggtatgtc gcgcataacg ccagctttga ccgccgcgta ctgcctgaga tgcccggtga
    18241 gtggatttgc actatgaaac tggcccgtcg tttgtggcct gggatcaagt acagcaatat
    18301 ggcgttatat aaaacacgca agctcaatgt acagacgccg ccgggcctgc atcatcaccg
    18361 cgcgttgtat gactgttata tcaccgccgc gttgcttatc gatattatga acacctccgg
    18421 ctggacggca gaacagatgg ccgatatcac cggacgtccg tcgttgatga cgaccttcac
    18481 ctttggcaaa taccgtggca aagcggtttc cgacgttgcc gaacgcgatc cgggctatct
    18541 gcgctggtta tttaataacc tggacagcat gagcccggag ctgcgtttaa cactgaaaca
    18601 ttatctggaa aatacttagt ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac
    18661 cagaaaagca tattccatcg ctacgccttc gtacgattta aagcgaccag atttaccgcc
    18721 atggcctgag tccatgtcgg tacagagcaa taaaagatgg tcatcggttt tcagctcgcg
    18781 caatttagcg acccattttg ccggttccca atattgcacc tgagaatcgt gcaaaccggt
    18841 cgttaccagt aaatgcggat aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt
    18901 catgtactcg taatattgcg gatcctgcgg gttaccccac tcttcaaact caccagtggt
    18961 aagaggaatt gattcatcaa gcatcgttgt tacaacatca acaaacggta cctgggcgat
    19021 aacgccgtgg aataattccg ggcgttgatt aattgcaacg cccatcaaca tgccccccgc
    19081 actcccgccc atcgcataac aaagcgaagg agagccatag cccagtttta acaatgcatc
    19141 gcaggcatca agataatcat taaacgtatt tttcttcttc agaaattttc cgccttcgta
    19201 ccattgttgc cccagctcac caccgccgcg aacatggaca atggcgtaga caaagccacg
    19261 atctaacaaa ctcaagcggc taaaactgaa atcggcatca atacttgcgc cgtaagaacc
    19321 atagccatac accagcaacg ggttgtgtcc tttgcgaaaa tgtttgcgat ggtagaccaa
    19381 cgaaaccgga acttcgacgc catcacgggc gactatccac aggtgttcac tgcggtaatt
    19441 cgccgcataa aaaccaggaa cttccgtttg ttttaataca cgacgctcac cggtatccat
    19501 atccagttca aacaaagtgt ctggtgtagt catggaagaa taaccataac gcaatcgcgc
    19561 ggtttcaggt tctggattgt aggcaatcca ggtcacatag gccggatcat caaaggcaat
    19621 accaatgact tcccgggtct tgcggttaat ttggcgcaaa ctggttaacc cgcgctgacg
    19681 ctcttcaacc accagccagt cggtaaacag cgtaaaccct tccagcatga tgttttcgcg
    19741 tggcggaatt aactcttccc actgttgctc atcacgcata cgggtacggt ataagccaaa
    19801 gtttttgccg tggcggttgg aacgcagata aaaacgatgc tggtagtgat caaggctgta
    19861 ttcgtgatct ttgcggcgcg gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc
    19921 cagcaggcga acttcactgg tggtggcgct ggccaaatga atgactacat agtgcttcga
    19981 ggtcgtttta tgcaggctga cgtaataggt atcgtctttt tcttcgtaga tcagtttatc
    20041 atgcgatgct ggcgtaccga tggcgtgacg ccagacctga taaggcagca gcgtcaccgg
    20101 atgcttgcga acatagtaga aagtccagga gtcatttgcc cagacaaagc tgggttcaac
    20161 gttatccagc agttccgggt accagttacc agtttccaga ttacgaaaac gaatgccgta
    20221 ctggcgtcgg gaaagaaaat cttctgccag cgccataatg gtgttatcgg gcgtaatcgc
    20281 cattccgccc atcgaataaa actcactatg agctgcgcgc ttattggcat cgagcaatgt
    20341 ttcccactca tcccactctt cactgaatgc cgattgacgc tggtagatag catattcaca
    20401 gcctggttca taaatatgcc gatagcggta gccatttttg atgtagggcg cagaaacttc
    20461 tcgttgcgga atgcggtcga tgatttcctt taagatgcga tcctgcaagg cttgttgtga
    20521 ggccatcacc cgatgaccgt aactattttc ttgttgcagg tagtccagga cttctggctg
    20581 agaacgcgta tcgtcccgca gccagtagta attatcgatg cgcgtatcgc catgaagcgt
    20641 catggcgtgg ggaatgcggg cggcttttgg tagcatgtta ttgttctttc tggttgaaac
    20701 atcttataag ggtggcaaaa ctcaccgggg atgcaagcga aacaggggag tcattgctta
    20761 gatgatgaca ggtaatggcg cggatatcga atgttatgca gacagagaaa atcagcctgt
    20821 tcaaatggct gtgcgattct ggatagcccg aaatagtcaa cttcaggcta tccagagagc
    20881 ggaattattc cgccaaagtg cgtttttgct gttcgagatc gcgttcaatg ccgtcacgaa
    20941 catcctgggg gattttcagc gcgtcaccca gtgcattcag gtaactgcgt tccataaaat
    21001 ggtcaatatc aatagccgcg caactcagga aatagatttc cagcgcctct tcttcattgc
    21061 ggaccccggt agccaggcgt tgtggatcca gcggttgttc gattgcctgc tcaatgagta
    21121 cacgcccctg ctcttccacg ccggcttcac gcaattgctg gtcgattgcc gcacgttctt
    21181 tggcatcaat atgaccatca cttttagcgg caaagaccag cgcaaggatc aaacgttctg
    21241 tacgctcatc cagcggcgta ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc
    21301 gaattttatc tttgtattta ttccacagca ccgtacccgc taccgctccg ccgccaacca
    21361 gtaacgcgtt ggtgccatat ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca
    21421 gcccagccag tccgcctaat gcgcctggca ctaacagttt gcccaatccc tgatccgcag
    21481 acgaggaggt agaagaactg ctttgcccaa gcagggattg cagttgattt aaccagttag
    21541 ccatatttgc tcctcaataa ccatttttag tgcctgtagc atagcggatg aggatgtcag
    21601 aaaatgtata tcgagacaaa agatgcgcaa ataagcgggg cggtaggccg ttattcaaag
    21661 aaattcgcgc caggtaaggt atttactgta tatcaggtga taagggtatt ttaggtgaaa
    21721 gtagtttgtc gttttgagtg gcattgtttg atgctgattg cccgatgcga cgctgttgcg
    21781 tcttctcagg cctacgattg tggtgcgttc tggggcggac aaggcactcg cgccgcatcc
    21841 gccaacggtc aatcgaatta tcagaagcca ttaacgccgc tgatattccg ccattcccgc
    21901 tttgcagtcc acgctgacct ggtaatgaat atcggcactt ttaccacgca cggttagcgg
    21961 tactgaccat ttatcatctt taccctgaat gtcctgcaaa ctgacccaag ccacgggatc
    22021 ggcctgaccg acaatttttt gatcatctgc ccagcgcgcc acgcgatttt gttgataatc
    22081 acgttttacg ctcgcggcaa ttccggcagc atccagacct tcacactttg ggaaagtgac
    22141 cgacttgctg gtttcattat tggcagcgaa aactgatgcg caggcagaaa ccaacaacag
    22201 ccctaaaaac gcccctcttt ttttcatgtt tttctccata gcacaatgat tcaggagaaa
    22261 gcatggtaca aattgtcagg agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc
    22321 ttcggattca gcaccgacga cctgtgggga cggtaatttg ccggttttca gaatggtgct
    22381 cagcacatct ttttgttctg ccagccatag cgaaagggct tcacgttgct cgtcttccat
    22441 ttgcactgga gaatttgtca gccaggtgtc aagcagatcc gccgtatcaa gcattttatc
    22501 ataagcatcg gcttcctttt tgctggtaaa cgacattttc tcttcgccct cacgaatgac
    22561 tacgtattta acttcaaccg ccatttgcag cctctcataa taactgtgat tttatacagt
    22621 atatttcttt tcggttgaga aatcaacatc agcaataaag acacacgcaa acgttttcgt
    22681 ttatactgcg cgcggaatta atcaggggat attcgttatg acgttattag gcactgcgct
    22741 gcgtccggca gcaactcgcg tgatgttatt aggctccggt gaactgggta aagaagtggc
    22801 aatcgagtgt cagcgtctcg gcgtagaggt gattgccgtc gatcgctatg ccgacgcacc
    22861 agccatgcat gtcgcgcatc gctcccatgt cattaatatg cttgatggtg atgcattacg
    22921 ccgtgtggtt gaactggaaa aaccacatta tatcgtgccg gagatcgaag ctattgccac
    22981 cgatatgctg atccaacttg aagaggaagg actgaatgtt gtcccctgcg ctcgcgcaac
    23041 gaaattaacg atgaatcgcg agggtatccg tcgcctggcg gcagaagagc tgcagctgcc
    23101 cacttccact tatcgttttg ccgatagcga aagccttttc cgcgaggcgg ttgccgccat
    23161 tggctatccc tgcattgtaa aaccggtgat gagctcttcc ggcaaggggc agacgtttat
    23221 tcgttctgca gagcaacttg ctcaggcatg ggaatacgct cagcaaggcg gtcgcgccgg
    23281 agcgggccgc gtaattgttg aaggcgtcgt taagtttgac ttcgaaatta ccctgctaac
    23341 cgtcagcgcg gtggatggcg tccatttctg tgcaccagta ggtcatcgcc aggaagatgg
    23401 cgactaccgt gaatcctggc aaccacagca aatgagcccg cttgcccttg aacgtgcgca
    23461 ggagattgcc cgtaaagtgg tgctggcact gggcggttat ggtctgtttg gtgtcgagct
    23521 gtttgtctgt ggtgatgagg tgattttcag tgaggtctcc cctcgtccac atgataccgg
    23581 gatggtgacg ttaatttctc aagatctctc agagtttgcc ctgcatgtac gtgccttcct
    23641 cggacttcct gttggcggga tccgtcagta tggtcctgca gcttctgccg ttattctgcc
    23701 acaactgacc agtcagaatg tcacgtttga taatgtgcag aatgccgtag gcgcagattt
    23761 gcagattcgt ttatttggta agccggaaat tgatggcagc cgtcgtctgg gtgttgcgct
    23821 ggcgaccgct gagagtgttg ttgacgccat tgaacgcgcg aagcacgccg ccggacaggt
    23881 aaaagtacag ggttaaaccc ggcaaaaaaa cgctacaaaa atgcccgatc gtagatcggg
    23941 cgttttgact tttacagctt agcgccttct acagcttcac gcgccagctt agtaatgcgg
    24001 tcgtaatcgc ccgcttccag cgcatctgcc ggaaccagcc aggaaccacc gatgcacagc
    24061 acgcttttca gcgccaggta gtcacggtag ttagccggag aaataccacc cgtcgggcag
    24121 aaacggacct gggagaacgg acccgcgatc gcctgcaggg ctttcacgcc gccgttagct
    24181 tcagccggga agaatttgaa ctctttcaaa ccgtagtcca tacccagcat cagttcggaa
    24241 acagtgctga tccccggaat tagaggaata gtcccttcgg tagcagcttt cagcagcggc
    24301 tcggtcagac ccgggctaat tgcgaactgt gcacccgctt cagtgacttc tgccagctgc
    24361 tgtggattca gcaccgtacc ggcacccaca atcgcttcag gcacttcttt ggcgatagca
    24421 cggatagcgt caactgcaca ctcggtacgc agagtcactt ccagaacgcg caccccacca
    24481 gcaaccaacg cttttgccat cggcactgcg tgttccagtt tttttaccac gataaccggt
    24541 acaaccgggc cggtggtcag gattgattct gcacttgttt tccagttttt catcagagtt
    24601 ttctctcgcc tgattacaaa tttgtcgtct taaaaagtga tacaggttgc gccctgttcg
    24661 gcaccggaca gtttttcacg caaggcgctg aataattcac gtcctgtccc cacgcgtgac
    24721 gcgctcaggt caggaatgcg cggttcgcga gcagccagtt ccgcttcgtc taccagcagc
    24781 gtcagttcgc ctgtctgtcc attcacacga atgatgtccc cgtcgcgcac ttttgccagc
    24841 agcccgccat cgtaggcttc tggtgttacg tggatagctg acggcacttt acctgaagcg
    24901 ccggagagtc gtccatcggt aactaacgca attttgaaac accggtccaa taatacacca
    24961 agtggcggca tgagtttatg taattctggc attccgttcg cttttggccc ctgatgacgg
    25021 acaacaacga cacaatcgcg gtccagcaaa cccgcttcaa aggccggcat aacgtcatgc
    25081 tggctttcaa aaacaaccgc tggcgcttca atcacctggt tctcaaccgg cacggcagag
    25141 gttttcataa ccgcacggcc caggttaccg cttaacactt ttgtcccacc atgatgagag
    25201 aaaggttgtt cgaaggaagc gatcacattg ctgtcgagtg atttttccgc cccttcccgc
    25261 cagtccagtt caccattatt cagccatggt tcaagggtat aacgagacag accaaagcct
    25321 gccaccgtat tgacatcttc atgcagcagg cctgctttga gcagttcacg caccagaacc
    25381 ggtacgccac ctgccgcctg gaagtggtta atatcggccg gaccgttcgg gtagagacgt
    25441 gccatcagcg gtacaacatc agaaaggtca gagaagtcat cccagttaat ctgaataccg
    25501 gccgcgcgcg ccatcgccac caggtgcatg gtgtggttag tggaaccacc ggtcgccagc
    25561 agtgcaacga taccgttcac caccactttc tcatcgatca tcttaccgat cggcatccat
    25621 tcattaccat taccggtcat gcgtgtaacc tgacgcgcag cggcagcggt caaagcatcg
    25681 cgcagcggag aatccggatg aacaaaagaa gagcctggca actgcatccc cataaattcc
    25741 accaccatct ggttggtgtt ggcagtaccg tagaaagtac atgttcccgg cgcatggtaa
    25801 gacgcggctt ctgactccag taaggccatg cggtccactt taccttcggc ataaagctgg
    25861 cgaatacgca ctttttcttt atttggcaaa ccgcttgcca tcggtccaga cggcacaaac
    25921 accgcaggca aatgaccaaa cgacagggct gccatcgtca gacccgggac aatcttgtcg
    25981 cacacaccga ggaacagagc accatcaaac atgttatggg acagccccac cgccgcagac
    26041 atcgctatca cttcgcggct tagcagcgac aattccattc catcctgccc ctgggtgaca
    26101 ccatcacaca tcgccggaac accgcccgca acctgaccaa ccgcattcgc ttcatgcagg
    26161 gctttacgaa tgatttctgg atagtgttca taaggctggt gcgcggagag catgtcgtta
    26221 taggaggtga tgatggcgat attgttacgc aacatgcttt tcaaagaggc tttgtcttct
    26281 ggctggcagg cagcgaaacc gtgtgccagg ttaccgcatg ccaactgcga acgatgaacg
    26341 gtcgacgttt tcgcttgttc tatccgggcg agataagcag agcgagtctc gcgcgaacgt
    26401 tcaatgattc gatttgttac gcgtaacaat tgtggattca taaaggctcc tgaaattgag
    26461 ttgtcagagc aggatgattc acaacgcgtt tcattcagag gatttatgag tgaaacgcct
    26521 gtaaccggag ctcatagggc aaaaacgttt cagtcagtgt aataaaaaaa gcctcgtggg
    26581 tgaatccgca cgaggcctga aagtgtaaaa attgttctac aatctgtgca agatcatgtt
    26641 accggtaaaa taaccataaa ggataagcgc agatattact caaactcatt ccaggaacga
    26701 ccatcacggg taatcatcgc caccgaggca acgggtcccc aggttccggc ctgatacggt
    26761 ttcggcgcat cattgtccat cgcccacgcc tcagtaatgg agtctaccca tttccaggct
    26821 tcttccactt cgtcgcgacg aacaaacagt gcctgaatac cacgcatggt ttccagcagc
    26881 aaacgttcat aggcatccgc cagatgcgtc tgattaaagg tttctgaata gctcagatcc
    26941 agcttggtga tttgcaggtt atgtttgtgg tcaaggccag gaactttatt cagtacctgg
    27001 atatccacgc cttcatcagg ttgcagacgg atagtcagtt tattctgcgg cagatcctgc
    27061 cacgactctt taaacagatt cagttcaggt gttttgaaat agaccacgac ttcagaacat
    27121 ttggtcggca gacgtttacc agtacgcagg tagaatggca caccggccca gcgccagtta
    27181 tcaatgtcga cgcggatcgc cacgaaggtt tctgtattgc tgctcttgtt cgcgccctct
    27241 tcttccagat atcccggcac ttttttgccc tgggcgaagc ccgcagtata ttgcccgcgt
    27301 acggtttttt cgcgtacgtt ggagcggtcg atgcggcgca gagacttcag tactttcact
    27361 ttttcatcgc ggatgctgtc tgcgctcagg tcagacggcg gagacatcgc aatcatgcaa
    27421 agaatttgca gcaggtggtt ctggatcatg tcgcgcatct gaccggcttt atcaaaatag
    27481 ccccagcgcc cttcgatccc cacttcttct gccacggtaa tctcaacatg atcaatggtg
    27541 cgattgtccc agttattcac aaacagggag ttagcaaaac gcagcgccaa caggttcagc
    27601 accgtttctt taccaagata gtggtcgata cggtaaacct ggcactcctc gaagtattcg
    27661 ccaacctgat cattgatttc ctgcgaggtc gccagcgacg tccccagcgg tttctccatg
    27721 actacgcgtg ccggtttagc attcagtttt gcctcgccaa gccctttgca aattgcgcca
    27781 aaagtgctgg gcggcatggc aaagtagtta atggtgatac gatttttttg atccagcatc
    27841 gcgccgagac ggctgaatgc agcagtgtca ttgacatcga gattacaaaa atccagacgt
    27901 gcactcaggg tgtcccataa accttcatca atggtttctt tcatgaaagt ttcgagcgcc
    27961 tcgcggacaa ctttggtata tgccgcttta tcccagtcag cacgccctac gccgataatc
    28021 cgggtgtccg ggttgagctg accggctttt tccagttgat acagggaagg cagcaattta
    28081 cgacgcgcaa ggtcgccttt cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc
    28141 gttaccgcca tgtcattctc cttaagttaa ctaacccggt acttaagcca gggtatactt
    28201 gtaattttct tacggtgcac tgtactgctt ttacgagctt gcgaaaactg taaacgctta
    28261 tccacccgtg cgattacggg aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat
    28321 cgttttccat tgggtaaaaa tctgacactg atcatgttat gaaaaaaaat aacaactttt
    28381 ttatctgctt ttgtcattaa cacggcacac aggcgtaata tctgacaaaa ctgcatttcg
    28441 atttctttca gtgcggaaat cgtcattacc cgtgagtctc tttacatcat gaatatgctg
    28501 gaaaaaatcc agtctcagct ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc
    28561 attctggctt cgcccgataa cgcgatccat tcgagtattg ctgctatggc actggaagcc
    28621 aatgttagcg aaccgacggt gaatcgtttc tgtcgcagca tggacacgcg cggttttcct
    28681 gattttaaac ttcatctggc acagagtctg gcgaatggca ctccctatgt taatcgcaat
    28741 gtcaatgaag atgacagcgt tgaatcatac acagggaaaa tatttgagtc cgcaatggca
    28801 acgcttgatc atgtccgtca ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg
    28861 ctcactcagg caaaaaaaat cgcctttttc ggattaggct cttcagccgc cgttgcccac
    28921 gatgcgatga ataagttctt tcgttttaat gttccggtgg tgtactccga tgatatcgtg
    28981 ctgcaacgca tgagttgtat gaattgtagc gacggagacg tggtggtgct gatttctcac
    29041 actggaagaa caaaaaatct ggtcgagctg gcgcagctgg ctcgcgaaaa cgacgccatg
    29101 gtgattgccc tcacctctgc gggtaccccg ctcgcccggg aagcaacgct ggcaattacc
    29161 ctcgacgtac cggaagatac tgacatttat atgcccatgg tttctcgact tgcacagctg
    29221 accgtgatag atgtgctggc gacaggattt actttgcgac gcggtgcaaa attcagagat
    29281 aacttgaagc gggtcaagga agcgctgaag gaatcgcgtt ttgataagca gttacttaat
    29341 ttaagtgacg atcgctaaaa acgactgtca ctgtcctaat cttatacgac atccgaatga
    29401 gattaattta tcgccatcgc ggcgttattt cattcggatt tcatgttcaa gcaacacctg
    29461 gttgtttcag tcaacggagt attacatgtc cagaaggctt cgcagaacaa aaatcgttac
    29521 cacgttaggc ccagcaacag atcgcgataa taaccttgaa aaagttatcg cggcaggtgc
    29581 caacgttgta cgtatgaact tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga
    29641 taaagttcgt gagattgccg caaaactggg gcgtcatgtg gctattctgg gtgacctcca
    29701 ggggcccaaa atccgtgtat ccacctttaa agaaggcaaa gttttcctca atattgggga
    29761 taaattcctg ctcgacgcca acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat
    29821 cgactacaaa ggcctgcctg ctgacgtcgt gcctggtgac atcctgctgc tggacgatgg
    29881 tcgcgtccag ttaaaagtaa tggaagttca gggcatgaaa gtgttcaccg aagtcaccgt
    29941 cggtggtccc ctctccaaca ataaaggtat caacaaactt ggcggcggtt tgtcggctga
    30001 agcgctgacc gaaaaagaca aagcagacat taagactgcg gcgttgattg gcgtagatta
    30061 cctggctgtc tccttcccac gctgtggcga agatctgaac tatgcccgtc ggctggcacg
    30121 cgatgcagga tgtgatgcga aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag
    30181 ccaggatgca atggatgaca tcatcctcgc ctctgacgtg gtaatggttg cacgtggcga
    30241 cctcggtgtg gaaattggcg acccggaact ggtcggcatt cagaaagcgt tgatccgtcg
    30301 tgcgcgtcag ctaaaccgag cggtaatcac ggcgacccag atgatggagt caatgattac
    30361 taacccgatg ccgacgcgtg cagaagtcat ggacgtagca aacgccgttc tggatggtac
    30421 tgacgctgtg atgctgtctg cagaaactgc cgcagggcag tatccgtcag aaactgttgc
    30481 agccatggcg cgcgtttgcc tgggtgcgga aaaaatcccg agcatcaacg tttctaaaca
    30541 ccgtctggac gttcagttcg acaatgtgga agaagctatt gccatgtcag caatgtacgc
    30601 agctaaccac ctgaaaggcg ttacggcgat tattaccatg accgaatcgg gtcgtaccgc
    30661 gctgatgacc tcccgtatca gctctggtct accaattttc gccatgtcgc gccatgaacg
    30721 tacgctgaac ctgactgctc tctatcgtgg cgttacgccg gtgcactttg atagcgctaa
    30781 tgacggcgta gcagctgcca gcgaagcggt taatctgctg cgcgataaag gttacttgat
    30841 gtctggtgac ctggtgattg tcacccaggg cgacgtgatg agtaccgtgg gttctactaa
    30901 taccacgcgt attttaacgg tagagtaagt acgttgccgg atgcggcgaa aacgccacat
    30961 ccggcctaca gttcaatgat agttcaacag atttcgaata ttctgaagca aacttgaact
    31021 tatcatcagg cgaaggcctc tcctcgcgag aggctttttt atttgatggg ataaagatct
    31081 ttgcgcttat acggctggat ttcgcccggt ttgcgagttt tcagcaattt tagtatccag
    31141 gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt cttcattcat ccgccgcgca
    31201 atcgtatgat catccgcctc taacagatca tccatcggtg ggcgcacctg aatcgtcaga
    31261 cggtgcgtct tgccatcata aatcggaaac agcggaacaa cgcgcgcacg gcacactttc
    31321 atcaaacgac caatcgcggg caacgtcgct ttataggtgg caaagaaatc aacaaattcg
    31381 ctgtgctcag ggccatgatc ctgatcgggt aaataatatc cccagtagcc ctgacgtacc
    31441 gactggatga atggtttaat accatcattt ctcgcatgca gacgaccgcc aaaacgacga
    31501 cgcaccgtgt tccagacata atcaaaaacc gggttgccct gattatggaa catcgctgcc
    31561 attttctgcc cttgcgaggc catcagcatg gcaggaatat cgacggccca accgtgcggc
    31621 accagaaaga taactttctc gttattacgc cgcatctctt cgatgatctc cagcccttgc
    31681 cagtcaacgc gcggctgaat tttctccggc ccgcgtattg ccaactcagc catcattgcc
    31741 atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg cttcgcgttc agcttcacta
    31801 cgttctggaa agcagagcga cagattgatt aacgcacgac ggcgtgagct ttttcccagt
    31861 cgtccggcaa aacgtcccag ccgtgccaga atgggatcac ggaactttgg cggcgttaaa
    31921 gcgatacccg ccatcgctgc tacgcccagc catgctcccc agtagcgcgg gtggcgaaag
    31981 gatttatcaa actcaggaat gtattcgcta ttattttttt tcgtttccat gcttttccag
    32041 tttcggataa ggcaaaaatc aatctggtga tagtgtagcg gcgcaacttg ccccgcacca
    32101 aataaaaaaa gccggtactg acttcgtacc ggctgcgaat ggatgttaat taatcaaacc
    32161 gtagctgcgg cacaatctct ttggcctgcg ccaggaattc gcgacgatcg gagccggtca
    32221 gcccttcggt acgcggcagt tttgccgtca gcgggtttac ggcctgctgg tttatccata
    32281 cttcatagtg caaatgcggc ccggttgaac gtccggtatt accggaaagc gcgatacggt
    32341 cgccacgttt caccttctgt cccggtttca ccagaatctt gcgcaagtgc atataacgcg
    32401 tggtgtagct gcgaccatga cgaatagcca cataataacc tgctgcgcca ctgcgtttgg
    32461 caaccaccac ttcaccgtca cccactgaaa gcactggcgt accctgcggc atggcgaaat
    32521 caacacctct gtgtggtgca acgcgaccgg tcaccggatt agtacgacgc gggttaaagt
    32581 tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa gaatcctttc gccagaccag
    32641 taccgttacg atcgtagaat ttgccatctt cagcgcggat agcgtaataa tctttacctt
    32701 ctgaacgcaa acgtacgccc agcagctggc tttgctcacg tttaccatca agcatttctc
    32761 gagacattaa taccgcaaat tcatcgcctt ttttcagttt gcggaaatcc atttgccact
    32821 gcatggcttt aatcactgcg ctcacttcgg cgctggttaa accggcgttt ctggcgctgg
    32881 caacaaagct ccccccgacg gtacctttca gcagattgtt aacccactct ccttgctgca
    32941 tttcgctggt cattttaaaa ccgttagcgg cagtacggtc ataggttcgg gtttcacgac
    33001 gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc ggttaatgtc caggagagtt
    33061 gttgaccgat tttcaggtta cgcaattctt tgtcggcagc agccagttgg gtgatatcac
    33121 ccatatcaat accatactga ttgagaatgc tgcttagcgt atcgccagtg gaaacaacat
    33181 attcatgcac gcccgcttca ccggcgattt tgtcatccag ttcgtcctga ggaatggctt
    33241 catcttcttg tgcagcttga tcaatcggct cactggcttc aggtaagagc gaacgaattt
    33301 cgttctgttc cagctcaatg gttttgacaa ttggcgtggc atcgcggtga taaacatagg
    33361 gccgccagac agcgacggcc agagtaagaa cggtgagcga ccccaacata acgcggtgtg
    33421 gtcgcggtaa attattaaac gccagggcga cagagcgggc tatctgttgc acgtaatcac
    33481 ttcctcatta atctcctttc aggcagctcg catactggtt ggctaattga ctcaggaatt
    33541 ctgaatagct tgttttaccc agtttgatat tcgtccccag gggatccaac gttcccatac
    33601 gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg cctgaactgt ggctcagcaa
    33661 aaacgcaggt tgctttttgc tcaaccaact gtgttcttat ttcatgtaaa cgctgcgcgc
    33721 caggttgaat ctcagggtta acggtaaaat gaccaagcgg tgtcagtcca aactgttttt
    33781 cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt ccccttgagc ggcgcgagct
    33841 cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc aaaatccttc aggttggcgt
    33901 caagtttggc tcgactttgc ggcataagtt ccactaattt tccatggatt gcaaccgctg
    33961 tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt gaaatcgccg tgatggtgat
    34021 cttcgtcact tttttccgcg tggtcgtgat catcatcatc gccgtgaata cttttcatca
    34081 gtagcggttt cacatcttca agctgcgcaa tcgttacctg cttcgcttct ggtaatttgc
    34141 ttaccggttt ttgcataaac gcttccatct ccgggccaac ccaaacgact aagtccgcgt
    34201 tctgtaagcg ttttacatcc gatggacgca gtgaataatc atgttctgaa gcgccgtcag
    34261 gcagtaaaac ctccgtttct gttacaccat cagcaatggc agaagcgatg aacccaacgg
    34321 gtttaagcga agcgacaacg gcggcatctg cggcctgtgt tgcacctccc cagagagcgg
    34381 cggataatgc tgcgaaaaga agcgtttttt tatgtaacat aatgcgacca atcatcgtaa
    34441 tgaatatgag aagtgtgata ttataacatt tcatgactac tgcaagacta aaattaacat
    34501 gacaagtctg gtttccctgg aaaatgtctc ggtttctttt ggccaacgcc gcgtcctctc
    34561 tgatgtgtcg ctggaactta aacctggaaa aattttgact ttacttgggc caaatggcgc
    34621 aggtaagtcg acactggtac gggtagtgct cgggctggta acacccgatg aaggggttat
    34681 caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag aagctgtatc tcgacaccac
    34741 gttgccactg accgtaaacc gttttttacg cttacgccct ggtacacata aagaagatat
    34801 tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt aacgcaccga tgcaaaagct
    34861 ctctggtggc gaaacgcagc gtgtactatt agcgcgagca ttgttaaatc gcccgcaatt
    34921 attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat ggccaggtgg cgttatatga
    34981 ccttattgac caactgcgtc gtgaactgga ttgtggcgtt ttaatggttt ctcacgatct
    35041 gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg aatcaccaca tttgttgttc
    35101 cggcacaccg gaagttgttt ccctgcatcc ggagtttatt tcaatgtttg gtcctcgtgg
    35161 tgctgaacaa ctgggtatct atcgccatca tcataaccat cgtcacgatt tacagggacg
    35221 aattgttttg cgtcggggaa atgatcgctc atgattgaat tattatttcc cggttggtta
    35281 gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt cgtttgtagt ctggcgtcgt
    35341 atgtcttatt tcggtgatac gctggctcat gcctcattac ttggcgtcgc gtttggtttg
    35401 ttgctggacg tgaatccatt ctatgcggta attgccgtta cgctgctcct ggcgggcggt
    35461 ctggtatggc tggagaagcg tccacagctg gcgattgaca cgttattagg gattatggcg
    35521 cacagtgccc tgtcgctggg cctggtggtc gttagtctga tgtctaacat tcgtgttgat
    35581 ttgatggctt acctgttcgg tgatttgctg gcagtgacgc cagaagatct catcgctatt
    35641 gcgattggcg tggtcatcgt ggtggctatt ttgttctggc aatggcgcaa tttgctgtcg
    35701 atgacgatta gcccggatct ggcgtttgtt gatggtgtga aattacagcg cgtgaaattg
    35761 ttgttgatgc tggtgacggc attgacgatt ggtgtagcga tgaaatttgt tggcgcgttg
    35821 attattactt cactgctgat tattcctgca gctactgcgc gtcgctttgc ccgtacgccg
    35881 gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg cagtaactgg cggtttaacc
    35941 ttttccgcgt tttacgatac gccggcgggt ccgtcggtgg tgctatgtgc ggcactgtta
    36001 tttattctca gtatgatgaa aaagcaggcc agctaatcta tcgctgaaca catttgtcgg
    36061 atgcggcgcg agcgccttat ccgacctacg gttcgctatc tctggtaggc cggataagac
    36121 gcgaatagcg tcgcatcagg cacactgcca atgtcggatg cggtgcgagc gaccaatccg
    36181 acttacggca tttctggcgg cgttatgcca aagtgattcc acgcccgcgt cgtcgccata
    36241 cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca gataaggctc cagcacatcc
    36301 tcaatggttt cacgttcttc gccaatagcc gccgccaggt tatccagacc tacaggccca
    36361 ccaaagaatt tatcgattac cgccagcaac aatttgcggt ccatataatc aaaaccttca
    36421 gcatcgacat tcagcatatc cagcgcctga gcagcgatat ctgccgagat ggtgccatcg
    36481 tgcttcactt cggcgaaatc acgcactcga cgcagcagac ggttggcaat gcgcggcgta
    36541 ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag ccccataaag
    36601 cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc cagacgttgc
    36661 acaataccaa aacggtcgcg caacggtgat gtcagcgaac ctgcgcgtgt ggttgcacca
    36721 atcagggtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc ttcaccaatc
    36781 atgatatcca gctggtagtc ttccattgcc ggatacaaca cctcttcaac aactggcgat
    36841 agacggtgga tctcatcaat aaacagcacg tcatgcggtt caaggttagt gagcatcgct
    36901 gccagatcgc ccgccttttc cagcaccgga ccagaagtcg tgcgtaaatt aacgcccatt
    36961 tcattggcga caatgttggc aagcgtagtt ttacccaacc ccggaggacc aaaaatcaac
    37021 aaatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc catctgtgaa
    37081 cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcgaatggc gcgatctgct
    37141 acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat catcctttac
    37201 ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg tcagggcgag
    37261 cgattttgct caccatgcgg cttgcttctt gtggtttata gcccagcgcc accagcgcgg
    37321 caaccgcttc ttgttcagca tcgtcggtcg ccgggctggc aggagacgtg agcaccagat
    37381 cggcggctgg cgtaaagaga tcgccgtgca aacctttaaa tcggtctttc atttcgacaa
    37441 ttaaacgctc ggcggttttt ttaccaatgc ccggcagttt caccagtgct cccacttctt
    37501 cacgttcaac ggcattaacg aactgctgcg ctgacattcc ggagaggatc gccagcgcca
    37561 acttcgggcc gacgccgttg gttttgatta actctttaaa caacgtgcgc tcttgcttat
    37621 tgttaaagcc gtacagcagc tgcgcgtctt cacgcaccac aaagtgggtg aaaacgatcg
    37681 cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc acttcatagc
    37741 ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg atgcctctga
    37801 gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt atgataaaaa
    37861 aatgctggat agatatccag cgaaggatga agaaaacttg cgaggcgtcc caacgatctg
    37921 aaaaatggcg cagtataatt tattctacag gttatattgg aagcaaatat ttgaatatta
    37981 catattcagt gaagaaatgc gtaataaaaa tatacattgc gactcctgaa ataataaatt
    38041 ttttatgcta ttacgtatat tcatatctat ttcaatggaa tgacaacgtg aatattaatt
    38101 accctgctga atatgaaatt ggatatatcg tctttacatg tataggtgct gccttatttg
    38161 gtcaaatatc agctgcatca aattgctgga gtaatcacgt cgggatcatt atcggtcata
    38221 acggtgaaga cttcctggtt gcagaaagcc gggttcccct ttcaaccatc accacgctat
    38281 ctcgttttat taaacgttcg tctaatcaac gctatgctat aaagcgatta gacgctgggc
    38341 tgacagaaca acaaaaacaa cgaattgttg aacaggttcc ctcccggcta cgcaaacttt
    38401 accacaccgg ttttaaatac gaatcttcgc gccagttctg ttcaaaattt gtttttgata
    38461 tttataaaga agcgctatgc attccggtgg gtgaaataga aacgtttgga gaactgttaa
    38521 atagcaatcc gaatgcaaaa ctcactttct ggaaattctg gttcttaggt tctattccgt
    38581 gggagcgtaa aaccgtcacg ccagccagtt tgtggcatca tccgggtttg gtgttgattc
    38641 acgcggaggg agttgaaacg cctcagcctg aactgaccga ggcggtataa cttaacgcag
    38701 tcgccctctc gccaggttca gccgcgattc gctcatctgc atcgcattct gactaacgtg
    38761 gcagtgggtg atggcaatcg ccagtgcatc ggcggcatcc gcttgtggat tagcgggcag
    38821 tttcagcaag gtgcggacca tatgctgcac ctggcttttc tcggcgctac caatgcctac
    38881 caccgtttgc tttacctgac gtgccgcgta ttcaaatact ggcaactcct gattcaccgc
    38941 cgccacaatc gccacgccgc gcgcctggcc cagtttcagg gctgagtcag cgttcttcgc
    39001 cataaagact tgttcaatgg cgaaataatc aggctggaac tgggtgatga tttccgtcac
    39061 gcccgcatag atgagcttca gacgagacgg taaatcatcc actttggtgc gtatgcatcc
    39121 gctacccagg taggacagtt gcctgcctac ctggcgaatt acgccatagc cggtcacgcg
    39181 cgaacccgga tcaatgccga gaataatagc catcacgcgt ctccgttttg ctgtttagca
    39241 ggcctcatca gagagtcgct gcgacctcat cagagatttc accgttatgg taaacttcct
    39301 gcacgtcgtc gcaatcttcc agcatatcga tcagacgcat cagtttcggt gcggtttctg
    39361 catccatatc agctttggtg gacgggatca tggaaacttc cgcgctgtct gctttcagac
    39421 ctgccgcttc cagagcgtcg cgcactttac ccatttcttc ccatgcagtg tagacatcaa
    39481 tcgcgccgtc atcataggtc acaacgtctt cagcacctgc ttccagagct gcttccatga
    39541 tggtgtcttc atcgcctttc tcgaaggaga tcacgccttt tttgctgaac agataggcta
    39601 cggaaccatc tgtaccgagg ttaccgccac atttgctgaa tgcatgacgc acttcagcaa
    39661 cggtacggtt gcggttgtca gacagacatt caatcatgat tgccgtgccg ccaggaccgt
    39721 aaccttcgta gatgatggtt tccatgtttg catcatcatc accgcccaca ccacgtgcaa
    39781 ttgcgcggtt cagtgtgtca cgggtcatgt tgttagacag tgctttatcc accgccgcac
    39841 gcagacgcgg gttagcgtcc ggatcgccac cgcccagctt agccgcggtt accagctcac
    39901 gaatgatttt agtgaagatt ttaccgcgct tagcatcctg cgcagcttta cgatgtctgg
    39961 tgttggccca tttactatga cctgccataa aaatatctcc agatagccct gcctgttcag
    40021 gcagcgttaa ttacaaactg ttcaatcgcc tgccggttgc tccaggactt agtgagcgcc
    40081 gccgcagcag gcgcatcaag ccacttgtaa gccagatgtt cagtgaaaac gatctgccgc
    40141 tcgtgcggaa gcgcaagaca gaaccatgat tccgtattac gcgtcacgcc cggcgcatag
    40201 cgatgacgta aatgtgaaaa aatttcaaac tctaccgtgc gctgacagtc aattaaggtc
    40261 agttgttcag cgacaacatc aatggtgacc tcttccttta cttcgcgcat ggcagcttgc
    40321 ggcgcggttt caccctcttc cacgctgccg gttaccgact gccagaaatc gggatcgtca
    40381 cgccgctgca acatcagcac ccgtttcgta tcttgtgcat agatgaccac taagatcgaa
    40441 acgggacgct tatacacttt atcctccacg ctgcctcttc gttgactgcc ttcgctcatc
    40501 ccattcacat agttatctat gctcatggga gttcactcag ttgccgcctt gatgcaacgc
    40561 gaatgatttc gtgtatttga gtcatatcag ttattctcag ccttcttcac aacctgaatg
    40621 ctcagctcag ccagtgcagt cgggttagca aagctcggtg cttcagtcat cagacacgct
    40681 gccgccgtgg ttttcgggaa ggcgataacg tcacggatat tgtcggtgcc ggtcagcagc
    40741 atggtcagac ggtcaagacc gaatgccaga cctgcgtgcg gcggagtacc gtatttcaga
    40801 gcgtcgagca ggaagccgaa tttctcgcgc tgttcctctt cgttgatacc cagaatacca
    40861 aacaccgtct gctgcatatc accattatgg atacgtactg aaccaccgcc cacttcgtaa
    40921 ccattgatga ccatatcgta agcgttcgcc accgcatttt ccggtgcagc tttcagttct
    40981 gcagcagtca tgtctttcgg tgaggtgaac ggatggtgca ttgccgtcag gccgccttca
    41041 ccgtcgtctt caaacatcgg gaagtcgata acccacagcg gtgcccattt gctttcgtcg
    41101 gtcagaccaa ggtctttacc cactttcagg cgcagtgcgc ccatcgcgtc ggcaacaatt
    41161 ttcttgttgt cggcaccgaa gaaaatcata tcgccatctt gcgcggcagt acgatccagg
    41221 atggcttcga tgatttctgc attaaggaac ttcgctaccg ggctgttgat accttccaga
    41281 cctttcgcgc gttcgttaac tttgatgtaa gccagacctt tcgcgccgta gattttaacg
    41341 aagttaccgt attcgtcgat ctgcttacgg gtcagcgatg cgccgcccgg aacgcgcaga
    41401 gcggcaacgc gacctttcgg atcgttcgcc ggacctgcaa atactgcaaa ctcaacagat
    41461 ttcagcagat cagcaacgtc ggtcagttcc atcgggttac gcagatccgg tttatcagaa
    41521 ccataacggc gttctgcttc cgcaaaggtc attaccggga aatcgcccag atccacgccc
    41581 ttcacttcca gccacagatg gcgcaccagc gcttccatca cttcacgcac ttgcggcgcg
    41641 gtcatgaaag aagtttccac atcgatctga gtaaattcag gctgacgatc agcgcgcagg
    41701 tcttcgtcac ggaagcattt aacgatctga tagtagcggt caaagccgga catcatcagc
    41761 agctgtttga acaactgcgg ggattgtggc agcgcgtaga atttaccttt gtgcacacga
    41821 gaaggcacca ggtagtcacg cgcgccttcc ggcgtggctt tggtcagcat cggagtttcg
    41881 atgtcgagga agccgtggtc atccataaaa cggcgcacca ggctggtgat tttagcgcgg
    41941 gttttcaggc gctgagccat ttccgggcga cgcaggtcga ggtagcggta tttcagacgc
    42001 gcttcttcgg tgttgacgtg gttagagtca agcggcagaa catctgcgcg gttgatgata
    42061 gtcagcgagg acgccagcac ttcgatttcg ccggtcgcca tatcgcggtt aatatttttt
    42121 tcgtcacgcg cacgtacggt gcccgtgacc tgaatgcaga actcattacg cagttcagag
    42181 gccagcttta acgcgtccgc acgatccgga tcgaaaaata cctgcacgat accttcgcgg
    42241 tcgcgcatat cgatgaagat caggctacca agatcacgac gacggttgac ccaaccacac
    42301 agagtcacct gctgccccac gtgggacaaa cggagctgtc cacaatattc tgtacgcatg
    42361 agatatccct taacttagct gccggcggat gccccctgct gcgcaggtga ccaagtcgca
    42421 gcgttagctg tatgtcacaa ctgaatgaaa aaaggcggct attatactgg aaattctgcc
    42481 gcaccgtaag agcctggccc gcgctggaac gcctcgttac cactttatat cgggcctgaa
    42541 atcagactct acgccagttt gctataaagg tgttgcccga actcataaaa attaacaaaa
    42601 tttgtcgttc cgccatcggc taatcgcatt aaggtgtgag gcacgatttt gttttttcag
    42661 gagtcattat gcttgaactt aatgctaaaa ccactgcgct ggtggtgatc gatttacaag
    42721 aaggcatctt accttttgcc ggtggtccac atactgccga tgaggtagtt aatcgcgccg
    42781 ggaagctggc ggcgaaattt cgcgccagcg gtcagcccgt gtttctggtg cgcgttggct
    42841 ggtctgccga ttacgccgaa gcattaaaac agccggttga tgccccctcc cccgctaaag
    42901 tgttgcccga aaactggtgg caacatccag ctgcattagg tgcaactgac agtgatatcg
    42961 aaatcatcaa acgtcagtgg ggtgcgtttt acggtacgga tctggagttg caactgcgcc
    43021 gccgaggtat cgatacaata gtgttatgtg ggatctcgac caatatcggt gttgaatcca
    43081 ccgcccgcaa tgcctgggaa cttggcttta atctggtgat tgccgaagac gcctgtagtg
    43141 ccgccagcgc cgagcagcac aataacagca tcaaccatat ctacccacgc atcgcccgtg
    43201 tgcgcagtgt ggaagagatc cttcacgcgt tatgatttac atcggtctac cgcaatggtc
    43261 gcatcctaaa tgggtgcggt tggggatcac cagccttgaa gagtatgccc gccactttaa
    43321 ctgcgtggag ggcaacacca cgctttacgc cctgccgaaa cccgaggttg tcctgcgctg
    43381 gcgtgagcag accacagatg acttccgctt ctgttttaag tttccggcga ccatttcgca
    43441 tcaggcagca ttacggcatt gcgatgattt agtgactgaa tttttgaccc gcatgtcacc
    43501 gttggctccg cgcattgggc aatactggct gcaactgcct gccacattcg gcccacggga
    43561 gctgcctgcg ctttggcatt ttctcgattc tctccctggc gaatttaatt atggtgtgga
    43621 agtccgccat ccacagtttt tcgccaaagg ggaagaggaa caaacgctta atcgcggttt
    43681 acatcagcgc ggcgttaatc gggtgatttt agacagccgc ccggttcatg cagcatgtcc
    43741 acacagtgaa gctattcgcg acgctcaacg aaaaaaacct aaagttccgg tacatgctgt
    43801 actgacggcg acaaatccgc tgattcgttt tatcggtagt gatgatatga cgcaaaaccg
    43861 ggaattattt caggtctggt tacagaaatt agcgcagtgg catcagacca ctacgcctta
    43921 tcttttttta catacgccag atattgccca ggccccggaa ctggtacata ccctgtggga
    43981 agacttacgt aaaacgcttc cagagatcgg agcagttccg gctattccac agcaatcttc
    44041 tcttttctga atttgccacc tatcatagac aggtgcaatc ggccatttta aagggagttt
    44101 gtatggtaag cgcgctgtat gccgttttaa gtgcgttgtt attaatgaag ttctcttttg
    44161 atgtcgttcg cttgcgtttg cagtaccgcg ttgcctatgg cgacggcggt tttagcgaac
    44221 tgcaaagcgc tattcgcatt catggtaacg cggtggaata tattcccatc gcgattgtgt
    44281 tgatgctgtt tatggaaatg aatggcgcag aaacctggat ggtgcatatt tgcggcatcg
    44341 ttttgcttgc tggtcgtctg atgcattatt acggttttca tcaccgtctg ttccgctggc
    44401 gacgttccgg catgagcgcc acctggtgtg cgctgttgct gatggtgctg gcgaatcttt
    44461 ggtatatgcc ctgggagttg gttttctccc tgcgttagcg cacaatacgc cactttcttt
    44521 ttcccggatt tttacgttat gtctcaccgc gacacgctat tttctgcccc tatcgccaga
    44581 ctgggcgact ggacctttga tgaacgggta gctgaagtct tcccggatat gatccagcgt
    44641 tccgttcccg gctattccaa tattatttcc atgattggta tgttagccga gcgcttcgtt
    44701 caacctggta cgcaggttta cgatctgggt tgttctctgg gcgcggcgac gctctcggtg
    44761 cgtcgcaaca ttcatcatga taattgcaaa attattgcca tcgacaactc cccggcgatg
    44821 attgaacgct gccgtcgtca tattgacgcc tataaagccc ctacgccagt agacgtcatt
    44881 gaaggtgata ttcgcgatat cgccattgaa aacgcatcga tggtggtgct gaattttacc
    44941 ctgcaattcc tggatccttc cgagcgccag gcattactgg ataaaattta tcaaggactg
    45001 aacccaggcg gcgcgctggt gctttcggaa aaattcagtt tcgaagatgc caaagttggt
    45061 gaactgttgt tcaacatgca ccacgacttt aaacgtgcca acggttacag cgaactggag
    45121 atcagccaga agcgcagcat gctggaaaac gtgatgctga ccgattccgt ggaaacccat
    45181 aaagcacgcc tgcataaagc cggttttgag catagcgagc tgtggttcca gtgctttaac
    45241 tttggttcac tggtggcatt aaaagcagag gacgctgcat gatcgacttt ggtaactttt
    45301 attctctgat tgccaaaaat catctttcac actggctcga aacgctgccc gcgcagattg
    45361 ctaactggca gcgcgagcag cagcacgggc tgtttaagca gtggtccaac gcggtggaat
    45421 ttctgcctga aattaaaccg tatcgtctgg atttattgca tagcgtaacc gccgaaagcg
    45481 aagagccact gagcaccggg caaattaaac gcattgaaac gcttatgcgg aacctgatgc
    45541 catggcgcaa agggccgttc tcactgtatg gcgtcaacat cgataccgaa tggcgttccg
    45601 actggaaatg ggatcgtgtt ctgccccatc tttctgattt aaccggacgc accattctgg
    45661 atgtcggctg cggcagcggt tatcacatgt ggcgcatgat tggcgcaggc gcgcacctcg
    45721 cggtggggat cgaccctacg caactgtttc tgtgccagtt tgaagcggtg cgtaaactac
    45781 tgggtaacga tcagcgcgca catttgttac cgttaggtat tgaacagctt ccggcactga
    45841 aagcctttga taccgtcttt tcgatgggcg tgctttacca tcgccgttcg ccgctggagc
    45901 atctctggca gttgaaagat caactggtga atgagggtga actggtgctg gaaacgctgg
    45961 ttattgatgg cgatgagaac acggtgctgg taccaggcga tcgttacgcg caaatgcgta
    46021 atgtctattt catcccttcc gcgctggcgc tgaaaaactg gctgaagaag tgtggttttg
    46081 tagatatccg cgttgtggat gtgtgcgtta ccactactga agagcagcga cgcactgaat
    46141 ggatggttac cgaatctctg tccgatttcc tcgacccaca tgatccgagt aagacagttg
    46201 aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg caagccgtaa aggtctggca
    46261 ttactgccgg atgcggcttg aatgccttat ccggcctaca aacccttgct aattcaatct
    46321 attgcaggga ctatgtaggc ctgataagca tagcgcatca ggcagtttta cgtttgcata
    46381 acctcagcgc ccgtttccgg gcgctattca cttcttatgc ctgaatagct ggctgatcaa
    46441 acgccgttaa cttcggcgca ttgcccgtat atttttcgat atacaccagc gcagagttac
    46501 cggcacaggc atttgccagc tgcgagctgg ggatatccgc cgttaacacg ttcgcactgc
    46561 cgtttttaca caagccattt tccagatctg gccatgcacc ttcatgcacg cataccacgc
    46621 cttttttgat cccgtcagtc accaccgcgc ctgtcagaat ctgaccacgt ttgttccaca
    46681 cacgcaccag atcaccattc gcaataccaa agcgagcagc atcttcggtg tgaatagtga
    46741 ttggttcacg atctgcaacc gcatattttt tacgcagttc cgcatagtta agctgactat
    46801 gtaaacggtg tgccggatat gcggtcagaa gctgcaactg cttctcgtcg gcggtaccct
    46861 tccactcatc aggcgcaagc caggttgggt gtgccgggca atccttatag ccaaattttt
    46921 ccagcgtttt ggagtaaatc tcaattttgc cgcttggcgt acccagcgca tttttcaccg
    46981 gatcggcgcg gaaatcagca taacgaacgt actgttcgtt cttctcgctg cggcgcattt
    47041 cgatcagttt attttgctgc cagaaggcat taaacatcgg catagtcacg cgttgcgcac
    47101 gagcaccttt ctgagcggca tcatagaaaa atttcagcca cgccatttca tctttacctt
    47161 cggtatagat ctcttttccg ccaggtttca gtaattcagc aagatcggca aatacgtcaa
    47221 aatcgtt
//
LOCUS       DAZFVO020000018        44657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFVO020000018 DAZFVO020000000
VERSION     DAZFVO020000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.018"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            307..1077
                     /gene="yjjP"
                     /locus_tag="ACHKNB_RS04770"
                     /old_locus_tag="pgaptmp_000954"
     CDS             307..1077
                     /gene="yjjP"
                     /locus_tag="ACHKNB_RS04770"
                     /old_locus_tag="pgaptmp_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HGU4194519.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1068..1541
                     /gene="yjjB"
                     /locus_tag="ACHKNB_RS04775"
                     /old_locus_tag="pgaptmp_000955"
     CDS             1068..1541
                     /gene="yjjB"
                     /locus_tag="ACHKNB_RS04775"
                     /old_locus_tag="pgaptmp_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HGU4194520.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1648..2187
                     /gene="dnaT"
                     /locus_tag="ACHKNB_RS04780"
                     /old_locus_tag="pgaptmp_000956"
     CDS             1648..2187
                     /gene="dnaT"
                     /locus_tag="ACHKNB_RS04780"
                     /old_locus_tag="pgaptmp_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HGU4194521.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPKAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2190..2927
                     /gene="dnaC"
                     /locus_tag="ACHKNB_RS04785"
                     /old_locus_tag="pgaptmp_000957"
     CDS             2190..2927
                     /gene="dnaC"
                     /locus_tag="ACHKNB_RS04785"
                     /old_locus_tag="pgaptmp_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HGU4194522.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2976..3470
                     /gene="yjjA"
                     /locus_tag="ACHKNB_RS04790"
                     /old_locus_tag="pgaptmp_000958"
     CDS             2976..3470
                     /gene="yjjA"
                     /locus_tag="ACHKNB_RS04790"
                     /old_locus_tag="pgaptmp_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HGU4194523.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3724..6015
                     /gene="opgB"
                     /locus_tag="ACHKNB_RS04795"
                     /old_locus_tag="pgaptmp_000959"
     CDS             3724..6015
                     /gene="opgB"
                     /locus_tag="ACHKNB_RS04795"
                     /old_locus_tag="pgaptmp_000959"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HGU4194524.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFCQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHKNB_RS04800"
                     /old_locus_tag="pgaptmp_000960"
     CDS             complement(6154..7176)
                     /gene="lgoD"
                     /locus_tag="ACHKNB_RS04800"
                     /old_locus_tag="pgaptmp_000960"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HGU4194525.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7315..8229
                     /gene="lgoR"
                     /locus_tag="ACHKNB_RS04805"
                     /old_locus_tag="pgaptmp_000961"
     CDS             7315..8229
                     /gene="lgoR"
                     /locus_tag="ACHKNB_RS04805"
                     /old_locus_tag="pgaptmp_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HGU4194526.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8444..9805
                     /gene="lgoT"
                     /locus_tag="ACHKNB_RS04810"
                     /old_locus_tag="pgaptmp_000962"
     CDS             8444..9805
                     /gene="lgoT"
                     /locus_tag="ACHKNB_RS04810"
                     /old_locus_tag="pgaptmp_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194527.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9854..11518)
                     /gene="tsr"
                     /locus_tag="ACHKNB_RS04815"
                     /old_locus_tag="pgaptmp_000963"
     CDS             complement(9854..11518)
                     /gene="tsr"
                     /locus_tag="ACHKNB_RS04815"
                     /old_locus_tag="pgaptmp_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HGU4194528.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWAYYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11637..12083)
                     /gene="hpaR"
                     /locus_tag="ACHKNB_RS04820"
                     /old_locus_tag="pgaptmp_000964"
     CDS             complement(11637..12083)
                     /gene="hpaR"
                     /locus_tag="ACHKNB_RS04820"
                     /old_locus_tag="pgaptmp_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HGU4194529.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12355..13644
                     /gene="hpaG"
                     /locus_tag="ACHKNB_RS04825"
                     /old_locus_tag="pgaptmp_000965"
     CDS             12355..13644
                     /gene="hpaG"
                     /locus_tag="ACHKNB_RS04825"
                     /old_locus_tag="pgaptmp_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HGU4194530.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13641..15107
                     /gene="hpaE"
                     /locus_tag="ACHKNB_RS04830"
                     /old_locus_tag="pgaptmp_000966"
     CDS             13641..15107
                     /gene="hpaE"
                     /locus_tag="ACHKNB_RS04830"
                     /old_locus_tag="pgaptmp_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4194531.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15109..15960
                     /gene="hpaD"
                     /locus_tag="ACHKNB_RS04835"
                     /old_locus_tag="pgaptmp_000967"
     CDS             15109..15960
                     /gene="hpaD"
                     /locus_tag="ACHKNB_RS04835"
                     /old_locus_tag="pgaptmp_000967"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HGU4194532.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15970..16350
                     /locus_tag="ACHKNB_RS04840"
                     /old_locus_tag="pgaptmp_000968"
     CDS             15970..16350
                     /locus_tag="ACHKNB_RS04840"
                     /old_locus_tag="pgaptmp_000968"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HGU4194533.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16461..17264
                     /gene="hpaH"
                     /locus_tag="ACHKNB_RS04845"
                     /old_locus_tag="pgaptmp_000969"
     CDS             16461..17264
                     /gene="hpaH"
                     /locus_tag="ACHKNB_RS04845"
                     /old_locus_tag="pgaptmp_000969"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HGU4194534.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17275..18063
                     /gene="hpaI"
                     /locus_tag="ACHKNB_RS04850"
                     /old_locus_tag="pgaptmp_000970"
     CDS             17275..18063
                     /gene="hpaI"
                     /locus_tag="ACHKNB_RS04850"
                     /old_locus_tag="pgaptmp_000970"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HGU4194535.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18186..19562
                     /gene="hpaX"
                     /locus_tag="ACHKNB_RS04855"
                     /old_locus_tag="pgaptmp_000971"
     CDS             18186..19562
                     /gene="hpaX"
                     /locus_tag="ACHKNB_RS04855"
                     /old_locus_tag="pgaptmp_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HGU4194536.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19572..20462
                     /gene="hpaA"
                     /locus_tag="ACHKNB_RS04860"
                     /old_locus_tag="pgaptmp_000972"
     CDS             19572..20462
                     /gene="hpaA"
                     /locus_tag="ACHKNB_RS04860"
                     /old_locus_tag="pgaptmp_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HGU4194537.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20713..22275
                     /gene="hpaB"
                     /locus_tag="ACHKNB_RS04865"
                     /old_locus_tag="pgaptmp_000973"
     CDS             20713..22275
                     /gene="hpaB"
                     /locus_tag="ACHKNB_RS04865"
                     /old_locus_tag="pgaptmp_000973"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HGU4194538.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGSMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22293..22805
                     /locus_tag="ACHKNB_RS04870"
                     /old_locus_tag="pgaptmp_000974"
     CDS             22293..22805
                     /locus_tag="ACHKNB_RS04870"
                     /old_locus_tag="pgaptmp_000974"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HGU4194539.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23198..25348
                     /gene="btsT"
                     /locus_tag="ACHKNB_RS04875"
                     /old_locus_tag="pgaptmp_000975"
     CDS             23198..25348
                     /gene="btsT"
                     /locus_tag="ACHKNB_RS04875"
                     /old_locus_tag="pgaptmp_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HGU4194540.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25398..25601
                     /gene="yjiX"
                     /locus_tag="ACHKNB_RS04880"
                     /old_locus_tag="pgaptmp_000976"
     CDS             25398..25601
                     /gene="yjiX"
                     /locus_tag="ACHKNB_RS04880"
                     /old_locus_tag="pgaptmp_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4194541.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25612..26568
                     /gene="yjiA"
                     /locus_tag="ACHKNB_RS04885"
                     /old_locus_tag="pgaptmp_000977"
     CDS             25612..26568
                     /gene="yjiA"
                     /locus_tag="ACHKNB_RS04885"
                     /old_locus_tag="pgaptmp_000977"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HGU4194542.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(26646..29186)
                     /locus_tag="ACHKNB_RS04890"
                     /old_locus_tag="pgaptmp_000978"
     CDS             complement(26646..29186)
                     /locus_tag="ACHKNB_RS04890"
                     /old_locus_tag="pgaptmp_000978"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HGU4194543.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(29538..29702)
                     /gene="yjiS"
                     /locus_tag="ACHKNB_RS04895"
                     /old_locus_tag="pgaptmp_000979"
     CDS             complement(29538..29702)
                     /gene="yjiS"
                     /locus_tag="ACHKNB_RS04895"
                     /old_locus_tag="pgaptmp_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HGU4194544.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            29879..31291
                     /gene="yjiR"
                     /locus_tag="ACHKNB_RS04900"
                     /old_locus_tag="pgaptmp_000980"
     CDS             29879..31291
                     /gene="yjiR"
                     /locus_tag="ACHKNB_RS04900"
                     /old_locus_tag="pgaptmp_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4194545.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(31533..32477)
                     /locus_tag="ACHKNB_RS04905"
                     /old_locus_tag="pgaptmp_000981"
     CDS             complement(31533..32477)
                     /locus_tag="ACHKNB_RS04905"
                     /old_locus_tag="pgaptmp_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4194546.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            32944..34176
                     /gene="mdtM"
                     /locus_tag="ACHKNB_RS04910"
                     /old_locus_tag="pgaptmp_000982"
     CDS             32944..34176
                     /gene="mdtM"
                     /locus_tag="ACHKNB_RS04910"
                     /old_locus_tag="pgaptmp_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HGU4194547.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            34217..35497
                     /gene="yjiN"
                     /locus_tag="ACHKNB_RS04915"
                     /old_locus_tag="pgaptmp_000983"
     CDS             34217..35497
                     /gene="yjiN"
                     /locus_tag="ACHKNB_RS04915"
                     /old_locus_tag="pgaptmp_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HGU4194548.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            35613..36764
                     /gene="yjiM"
                     /locus_tag="ACHKNB_RS04920"
                     /old_locus_tag="pgaptmp_000984"
     CDS             35613..36764
                     /gene="yjiM"
                     /locus_tag="ACHKNB_RS04920"
                     /old_locus_tag="pgaptmp_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HGU4194549.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            36774..37541
                     /gene="yjiL"
                     /locus_tag="ACHKNB_RS04925"
                     /old_locus_tag="pgaptmp_000985"
     CDS             36774..37541
                     /gene="yjiL"
                     /locus_tag="ACHKNB_RS04925"
                     /old_locus_tag="pgaptmp_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HGU4194550.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTHR"
     gene            37538..37795
                     /gene="ytiA"
                     /locus_tag="ACHKNB_RS04930"
                     /old_locus_tag="pgaptmp_000986"
     CDS             37538..37795
                     /gene="ytiA"
                     /locus_tag="ACHKNB_RS04930"
                     /old_locus_tag="pgaptmp_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HGU4194551.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            37860..38720
                     /gene="yjiK"
                     /locus_tag="ACHKNB_RS04935"
                     /old_locus_tag="pgaptmp_000987"
     CDS             37860..38720
                     /gene="yjiK"
                     /locus_tag="ACHKNB_RS04935"
                     /old_locus_tag="pgaptmp_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HGU4194552.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            38788..39966
                     /gene="yjiJ"
                     /locus_tag="ACHKNB_RS04940"
                     /old_locus_tag="pgaptmp_000988"
     CDS             38788..39966
                     /gene="yjiJ"
                     /locus_tag="ACHKNB_RS04940"
                     /old_locus_tag="pgaptmp_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194553.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(39979..40533)
                     /gene="kptA"
                     /locus_tag="ACHKNB_RS04945"
                     /old_locus_tag="pgaptmp_000989"
     CDS             complement(39979..40533)
                     /gene="kptA"
                     /locus_tag="ACHKNB_RS04945"
                     /old_locus_tag="pgaptmp_000989"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HGU4194554.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            40783..41466
                     /gene="yjiH"
                     /locus_tag="ACHKNB_RS04950"
                     /old_locus_tag="pgaptmp_000990"
     CDS             40783..41466
                     /gene="yjiH"
                     /locus_tag="ACHKNB_RS04950"
                     /old_locus_tag="pgaptmp_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HGU4194555.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            41463..41924
                     /gene="yjiG"
                     /locus_tag="ACHKNB_RS04955"
                     /old_locus_tag="pgaptmp_000991"
     CDS             41463..41924
                     /gene="yjiG"
                     /locus_tag="ACHKNB_RS04955"
                     /old_locus_tag="pgaptmp_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HGU4194556.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            41937..43109
                     /gene="iadA"
                     /locus_tag="ACHKNB_RS04960"
                     /old_locus_tag="pgaptmp_000992"
     CDS             41937..43109
                     /gene="iadA"
                     /locus_tag="ACHKNB_RS04960"
                     /old_locus_tag="pgaptmp_000992"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4194557.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            43174..44085
                     /gene="hypT"
                     /locus_tag="ACHKNB_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /gene_synonym="qseD"
     CDS             43174..44085
                     /gene="hypT"
                     /locus_tag="ACHKNB_RS04965"
                     /old_locus_tag="pgaptmp_000993"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HGU4194558.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(44078..44470)
                     /gene="iraD"
                     /locus_tag="ACHKNB_RS04970"
                     /old_locus_tag="pgaptmp_000994"
     CDS             complement(44078..44470)
                     /gene="iraD"
                     /locus_tag="ACHKNB_RS04970"
                     /old_locus_tag="pgaptmp_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HGU4194559.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(44467..44550)
                     /gene="idlP"
                     /locus_tag="ACHKNB_RS04975"
                     /old_locus_tag="pgaptmp_000995"
     CDS             complement(44467..44550)
                     /gene="idlP"
                     /locus_tag="ACHKNB_RS04975"
                     /old_locus_tag="pgaptmp_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HGU4194560.1"
                     /translation="MENEHQYSGARCSGQTAYVAKRQECAK"
BASE COUNT        10413 a        11774 c        11472 g        10998 t
ORIGIN      
        1 acacaaccaa tatttattca atgaaatctg atggttttaa cttgttaaca ttaaaaaacg
       61 tgcccggtta tctattatcc tgattcgtaa aggccaaaca ataaattcat tatagtgata
      121 atattcatgt gatagtttgt ttgcggcgag agataattcg ctttttatca ccgagcttgt
      181 cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa acaaccgtgc
      241 ttcatgatac aaatggattc tcattatctg aacaatacgc agcacgtcta tgacaaaggg
      301 cgagttatgc aaactgagca acagcgagcc gtaacacggc tttgtatcca gtgtggatta
      361 tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc acgactgggt
      421 cgggcgttgg ggatggacag cgtcgaaagt tctatctctt cgaacgccat agtgctgaca
      481 accattaaag atgggcaatg cctgacatcg acacgtaaaa atcacgatcg cggcattaat
      541 atgcatgtgg tgactgaagt ccagcacatt gtgattctgg cggagcacca tctgctggat
      601 tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc aagatggctg
      661 gtagccttaa tggttggcct ttcttgtgcc tgtttctgta aactcaataa aggtggctgg
      721 gatggtgccg tcatcacctt ctttgccagt acggccgcga tgtatatccg ccagctgctg
      781 gcacaacgtc atcttcatcc acaaatcaac ttttgcctta ccgctttcgc cgccaccacc
      841 atttccggat tgcttttgca acttcccact ttcagcaata cacccactat cgcgatggcc
      901 gccagcgttc tgctgctggt gccgggattt ccgttgatta atgccgtcgc cgatatgttt
      961 aaaggccaca tcaataccgg actggcacgc tgggcgatcg ccagtctgct gacactggct
     1021 acctgcgtcg gcgtagtgat ggcactgacg atttgggggc tacgcggatg ggtgtgatcg
     1081 aatttctgtt agcgttggcg caggatatga tcctcgccgc cattcctgcg gtcggctttg
     1141 cgatggtgtt caacgttccc gttcgggcgt tacgctggtg tgcgctgctt ggcgcgattg
     1201 gtcatggttc acgaatgatc ttaatgacca gcgggctgaa tattgagtgg tcaactttta
     1261 tggcttctat gctggtcggc accattggta ttcaatggtc gcgctggtat ctggcgcatc
     1321 cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc gggcatatcg gcttataccg
     1381 caatgatttc ggcggtaaaa atcagccagt taggttacag cgaaccgttg atgattaccc
     1441 tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc ggtctttcca
     1501 ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc gcttaagcac
     1561 acggatgaga gacagcctcc tctcctccgt gtgttactat aaaagttatc tcccttctcg
     1621 ttcatcgttc catatttgag aaacagtatg tcttccagag ttttgacccc ggacgtcgtt
     1681 ggtattgacg ccctggtaca cgatcaccaa accgttctgg caaaagctga aggcggtgtg
     1741 gttgccgtat ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc acgcctggct
     1801 gaactgctgg cgctggaaga aaagctggcg cgtccgggaa gcgatgtcgc tctggacgat
     1861 caactctatc aggaaccgaa agccgctccc gttgctgtcc ccatggggaa attcgccatg
     1921 tatccggact ggcaacccga tgccgacttt atccgcctgg cggcgctatg gggcgtggcg
     1981 ctaagagagc cggtgaccac cgaagaactg gcctcattca ttgcctactg gcaggcggaa
     2041 ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt
     2101 cgtgccagca acggcggact gccgaaacga gatgtgaata cggtcagcga acctgacagc
     2161 caaattccac caggattcag agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc
     2221 tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa aacgggtgaa gagctactgg
     2281 cctggcagaa agaacaagga gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga
     2341 tgaaaatgca gcgcaccttt aaccgctccg gtattcggcc actgcatcag aactgctcct
     2401 ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt
     2461 acgtcgaaga gtttgacggc aacatcgcca gcttcatctt ttctggtaag ccaggaaccg
     2521 gcaaaaacca tctggcggcg gcaatctgca acgagctgct gctacgcggt aaatccgtat
     2581 tgatcatcac cgtggccgat attatgtcgg cgatgaaaga taccttcagg aatagcggta
     2641 ccagtgaaga acaactgctt aacgatctga gcaacgttga tctgctggtg atcgatgaga
     2701 tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc
     2761 gctcttcttc caaacgccca accgggatgc tgaccaacag taatatggaa gagatgacca
     2821 agttgctggg cgaacgcgtg atggaccgta tgcgcctggg taacagtttg tgggtgatct
     2881 tcaactggga tagctaccgt agccgtgtaa caggtaaaga gtattaatcc ccgtttctgg
     2941 cggtatactg cgacacaatt ccaccttgag taatgatgat gaaaactgtt aaacatcttc
     3001 tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga
     3061 aagatgcgct ctccagcgcc gccagcgaac ttggaaacca aaacagcacg acacaggaag
     3121 gcggttggtc gctcgcgtca ttaactaact tgcttagcag cgggaaccag gccttaagcg
     3181 cagataacat gaacaacgcc gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt
     3241 cggtaaccga tgccgaaaac atcaagaacc aggtgctgga aaagctgggc ctgaacagtg
     3301 aagagcaaaa agaagacacc aactatctgg atggcattca gggtttgctg aaaaccaaag
     3361 atggtcagca actcaatctg gataacatcg gaacgactcc gctggcagaa aaggtgaaaa
     3421 ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt catttcctga accgcgaaac
     3481 gccgcgtttt agctgttacc cggctgacgc ggctctctcc tgcccttttc cctgctcctg
     3541 gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtctg tttataaact
     3601 aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg cgacgccatt
     3661 acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc
     3721 ccgttgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg
     3781 aaagcgggac gtaacacctg gtggtttgca gccacattaa cggtgctggg gctatttgtc
     3841 gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat taacgacgcg
     3901 gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata catcctgccg
     3961 ggtatcggca ttgtgctggg gctgacagcg gtgttcggtg cgctgggctg gatcctgcgc
     4021 cgtcgtcgcc atcatccgca ccattttggt tacagcctgc tggcgctctt actggcgctg
     4081 ggttcggtgg acgccagccc agcattttgt cagataacgg aactggtgaa atcccagtca
     4141 cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac tatccctgac
     4201 ccgaaactca acctggttta tatctacggc gaaagtctcg agcggaccta ttttgataac
     4261 gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg cctggatttc
     4321 agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt ggcttctcag
     4381 tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc
     4441 ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca gaactatttc
     4501 gtgcagggcg cgaatctgcg ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc
     4561 gaccacttat acggctcaga agagctgaaa agcgtggtgg ccgacccgca ctatcgcaac
     4621 gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt
     4681 tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca tcacccggat
     4741 ggttttatct cgcgtacctg taaccgcaaa aaatatgatt ttgacggtaa gccgaaccag
     4801 tcattcagcg cggtaagttg cagccaggag aatatcgcgg cgtttatcaa caaaatcaaa
     4861 gcgtcaccgt ggtttaaaga taccgtcatc gtcgtctctt ctgaccattt agcgatgaac
     4921 aacacggcgt ggaaatacct taataagcag gatcgcaata acctgttttt tgtcattcgt
     4981 ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta acacgatgga taacggcgcg
     5041 acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag cagtttatcc
     5101 gggcagtcaa tgtcggaaat cttcctcaat atcaaagaga aaacattggc gtggaagccg
     5161 gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat cgaccagcag
     5221 aaaaacatga ttgccttctc gggtagccat ttccgtttgc cgctgctgtt gcgggtttca
     5281 gacaagcgcg tagaaccgct gccggaaagt gaatactcag caccgctgcg tttccagctg
     5341 gccgatttcg ctccacgcga caatttcgtc tgggttgacc gttgctacaa gatggcacaa
     5401 ctctgggctc cggaactggc actctccacc gactggtgtg tctcgcaggg gcagcttggc
     5461 ggtcagcaaa ttgtccagca tgttgataaa acaatgtgga agggcaaaac ggcatttaaa
     5521 gatacggtca tcgacatggc gcgttacaaa agcaatgtcg atacgctgaa aattgttgat
     5581 aacgatattc gctacaaagc cgacagtttc atcttcaacg tcgccggtgc gccagaagag
     5641 gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg gccgctggtc caacgctcag
     5701 ctgggcgatg aagtaaaaat cgagtacaag catccgctgc cgaagaaatt tgacctggtg
     5761 attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg cgtaggcaat
     5821 gaagaacaaa ctcttgtgct gggcaatgaa gtaaccacca ccacactgca tttcgataac
     5881 ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg
     5941 aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat taaagttgtg
     6001 gaacgtgaag ggtaatttct gactgatgtg taggtcgcat aaggtgcgac ctacacacct
     6061 tctccggcct acaaaacatc attgagaaac agaaaaaaac agcgcccaac acttccccgg
     6121 acgctgcatg gcaactttaa taatctcgcc ttatcagaaa gtaattacac ccttaatcaa
     6181 ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga acggatagcg
     6241 atgggttaac atcatgtcgg cagtgatttt cccttctgcc atcagtcgac cgactttagc
     6301 gaaatcttcc ggcgtggcgt tgcggctgcc catcatcgtc gtttcttttt tatggaattc
     6361 cggatcggag aactgcaact cacctttaaa caggccgaca aataccaccg tgccgccgtg
     6421 acgaatcaaa ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt cgatcacttt
     6481 ctgcgccagc gaaccaccaa actgcgcccg tagctgcgcg tcaaaatctt cggctgacgg
     6541 gtccagtaaa ggtaattcca gacgcgttgc cacatgttca cgacgcgccg gactggtatc
     6601 cgccaccacc acctgtgcgc catcggcttt agcgattgcc gccgcgccca gaccgattgg
     6661 ccccgccccg accaccagca cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg
     6721 agcgctaata gcgaaaggtt caatcaatgc tgccgcctgc gggtcaatac cgtctgcggg
     6781 caaaatgttc gccaccggca cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc
     6841 aatgactgaa attttttcac agcaattggt acgcccgctt ttacacgccg ggcattgctg
     6901 acaggcaaca taagggatca cagcaacttg ctgaccattt ttaagatcag caatattttt
     6961 acccagccca acaatctccc cacatatttc atggcctaaa acacgtggat aactaaaaaa
     7021 tggttgattt ccaccccagg catgaatatc ggtaccgcaa atcccgacag acttaatttt
     7081 tattaatgct tcattgtcac ccggaatagg tatctcgcgt tgtttccaga ctaattcttt
     7141 cggctgctgg caaattaaaa cattcatcgt agacatgatt cggctctcca ttttttgttg
     7201 ccttagatat caatgaaaat gaaaaatggc agcggtacgg aaactctgtt ttgtgaaaac
     7261 acacgcataa aaatgtttta aatcgggttt taatggactc acaaggagga aaatatgagt
     7321 cgttcacaaa atttacgcca caatgtgatt aaccaggtca ttgatgatat ggcgcggggt
     7381 catatccctt ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc
     7441 accacagtgc gtcatattct cagccattta cgcgaatgcg gcgtcttgac gcaggttgga
     7501 aacgactatg ttattgcgcg taaacctgac cacgatgacg gtttcgcctg tactaccgct
     7561 tcaatgagtg aacaaaacaa agtgtttgag caggcttttt tcacgatgat aaaccagcgc
     7621 cagttacgcc caggggaaac tttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc
     7681 ccggtggttg taagagaata tcttttaaaa ttcggccgtt ataatcttat tcaaagcgaa
     7741 aagcgcggcc agtggagcat gaagcaattc gatcaatcct acgccgagca gctgtttgag
     7801 ctacgcgaaa tgctcgaaac acattcactt cagcactttc tcaacctgcc tgatcatgat
     7861 ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg tgacaacatc
     7921 ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt actgctttca
     7981 gctgccgaca atatcttttt tgatcaatcg cttgagatca tctcagtcat ctttcatttt
     8041 cactatcaat gggacgaaag cgatcttaaa cagcgcaata tcattgccgt cgatgagcat
     8101 atgaccattc tcagcgctct gatttgccgc agtgaccttg atgccaccct tgcgctccgt
     8161 aaccatttga attcagctaa acaatcaatg attcgatcca ttaatgagaa cacgcgttat
     8221 gctcattaag tacggattaa aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag
     8281 ataaataccg ggaaattccc gcttacctat gctcacaatc agtcgactaa aaatgttttt
     8341 gtgagcggta gtaaagtcct aaaactttaa cctgccgtta atgtcaacct tgcgtaattc
     8401 acaattacgt aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat
     8461 caccctcgat ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc
     8521 agatggatta gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt
     8581 attatttttt gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt
     8641 aacgattcgt gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt
     8701 ttcactcgct tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc
     8761 acgcctgatg ctgggactgg ggatgttctt ctggtcactg ttccaggcaa tgtctggcat
     8821 ggtgcacaac tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc
     8881 gatgaaccca tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg
     8941 cccgatgggc ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct
     9001 ggcggcgatg atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg
     9061 gatttttctc gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac
     9121 tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag
     9181 ttttgccgaa tggcgcagcc tgttccgtaa ccgtacaatg tggggaatga tgctcggatt
     9241 cagtggcatc aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac
     9301 agcctataac ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg
     9361 ggctgccggg atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc
     9421 tccgattaaa agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac
     9481 gctgatagta ccacaagcga caacatccat gacggcggtt ctgctgattg gcatggcact
     9541 gttctgtatt cactttgccg gaacatcctg ctggggcttg atccacgtcg cagttgcttc
     9601 tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc agcttcatct gcgcctcttt
     9661 tgcgccgatc attactggtt ttattgttga taccacccac tcattccgtc tggcactaat
     9721 catctgcggt tgcgtcaccg cagcgggggc actggcgtac atcttcctgg ttcgtcagcc
     9781 gatcaacgac ccacgtaaag attaatctcc ctatgcccga taacattttg cttatcgggc
     9841 atttttatgg cgattaaaac gtttcccagt tctccccgct atctgccact gccattttac
     9901 gcggcgtagc tggcgtcacg gtttttacca cagccgatgt ttcacgctgc tgttgctgct
     9961 gttgctgaat ccggaacact gccacagctt cggtcaggcg actggcctgc tcttccagcg
    10021 cggcggcggc ggcggcagat tcttccacca gcgcggcgtt ctgttgagtt acccggtcca
    10081 tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca gaagcagaag
    10141 ctatttcgcc cataatgtcc gtcacgcggg tcacagcact gacaatctcc gccattgttt
    10201 ccccggcgct ttcgaccagc gtagagccaa catccacttt ccccaccgag tcttcaatca
    10261 ggcttttaat ttcacgagcc gcctgggcgc tacgctgggc cagattacgc acttctcccg
    10321 caaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc agcctcaacc gccgcgttca
    10381 aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata tcggcgattt
    10441 tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatccacc actttgccgc
    10501 cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct ggcctggcgc gcattttcgg
    10561 cgttctgctt caccgttgcg gtcagttgct ccatgctggc tgccgtctct tccagcgaag
    10621 cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtggcgatt tcgctggcac
    10681 cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc agctctccct
    10741 gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc acctcaatcg
    10801 gtttcaccag atcgccgcct gcaatatgac gaatgctgtc aatcaggcga ttcattggcg
    10861 ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt acgacgatca
    10921 tcacgcccac cagaatccac atagcctggc tgtaggaggc attgttatcg ctgacggcga
    10981 tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg aaaccgtcct
    11041 gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct aatagttgga
    11101 tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct gccgctgcgg
    11161 cggtgctctg acgcgggtca cgcggcaacg cttcgtaata cgcccagttt ttttccgcct
    11221 gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg ctaccaatat
    11281 tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc gtctgcaaca
    11341 acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt aaaacagtga
    11401 aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc agttgtaaaa
    11461 ggccaaaaac agccaaaacc agcagtaagc tggtcacgat cttgatacgt tttaacatgg
    11521 tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa ctttatgaaa
    11581 aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt atcagatact aaaaagttat
    11641 tcattatcgc ccgggatgtc ttcttgtcgg gaattgccca gagcaataaa ttcctctaac
    11701 agatgggtta actgttgcat tttctcggca gtaaactgag cttcaatctg ccgataggct
    11761 tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt tagcgagata
    11821 tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc gcgttccatc
    11881 cgcgtaagga tcccggtcag gcttgggcgc aaaatgcagg cgcgatacgc cagatcgtga
    11941 aaatccattg atgggctttc cgccagaata cgcacgatgc gccactgttg ctcagtcaaa
    12001 ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc ctggagcaac
    12061 gcaatggtta gagagtcgtg cattatcttt cccctgaaaa attcaaaatc attaatatag
    12121 aaacagttaa tcatcacgtt accgggactg cgctgcccct gcaattcttc attttatcaa
    12181 aattgagcat gttttgttaa cttgctgata tttaatattt tattatcttt aaatgtaaat
    12241 agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc aatcgggaat
    12301 aaaagtaatc attaacatat taatgattaa aatgatcgaa tccgaggagt ggtaatgaaa
    12361 ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg gcaggaagcg
    12421 ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat taaaccgcgc
    12481 aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa ggtactgagc
    12541 ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga aaatacgtga agaagatgca
    12601 gcagagtaca tcgccggata tgcgctggct aacgatgtca gcctgccgga agagagcttt
    12661 taccgcccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg cgaaaccgtg
    12721 gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg tcctgccgat
    12781 cactggaata ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc cctgagcgaa
    12841 tttgccacac tgaatccagg cgatgccatt ctgctcggca cgccacaggc gcgcgtggaa
    12901 atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct ggaaaatccg
    12961 gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgcagcc acacccgcac
    13021 ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact ggaatttaag
    13081 ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg cgataaccag
    13141 acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct ggtggtagtt
    13201 attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt cgcgggctac
    13261 accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg ccctaacctg
    13321 cgggtcaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc gaaagaggcg
    13381 atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt acgccagcaa
    13441 ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt aagcgaattt
    13501 atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt atctgacgtg
    13561 gtgcctggcg atgaagtggt ggtggaagta gaaggcgtgg gccgcctggt gaaccgaatt
    13621 gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg gatcaacggt aaaaatgttg
    13681 caggtaacga ctacttccag actaccaatc cggcaacggg tgaagtgctg gcggatgtgg
    13741 cctccggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag gcgttcccga
    13801 aatgggccaa tctgccgatg aaagagcgcg cgcgcctgat gcgccgtctg ggcgatctga
    13861 tcgaccagaa cgtgccggag atcgccgcga tggaaaccgc ggacacgggc ctgccgatcc
    13921 atcagaccaa aaatgtgttg atcccacgcg cttctcacaa ctttgaattt ttcgcggaag
    13981 tctgccagca gatgaacggc aagacttatc cggtcgacga caagatgctc aactacacgc
    14041 tggtgcagcc ggtaggcgtt tgtgcactgg tgtcaccgtg gaacgtgccg tttatgaccg
    14101 ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg aaaatgtcgg
    14161 aactctcccc gctgaccgct gaccgcttgg gtgagctggc gctggaagcc ggtattccgg
    14221 caggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggggatgcg ctggttcgtc
    14281 atcatgacgt acgtgccgtg tcgttcaccg gcggtacggc caccgggcgc aacatcatga
    14341 aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg taaatcgccg gtgctgattt
    14401 ttgaagatgc cgatattgaa cgcgcgctgg acgccgccct gttcaccatc ttctcgatca
    14461 acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc tacccggaat
    14521 tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg aacgatccga
    14581 atacccaggt tggcgcgctt attagccagc agcactggga aaaagtctcc ggctatatcc
    14641 gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa ccgtccgacc
    14701 tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca gacgttgata
    14761 accgtatgcg agtcgcccag gaagagattt tcgggccggt cgcctgcctg ctgccgttta
    14821 aagacgaagc cgaaggctta cgtctggcaa acgacgtgga gtacggcctc gcgtcgtaca
    14881 tctggacaca ggatgtcagc aaagtgttac gcctggcgcg tggcattgaa gctggcatgg
    14941 tgttcgtcaa cacccagaac gtgcgtgacc tgcgccagcc atttggcggc gtaaaagcct
    15001 ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa atgaagaacg
    15061 tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat gggtaagtta
    15121 gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact gccagggaaa
    15181 aaccacggtt gccgccaggg cgcgatcgac gggcataaag agatcagcaa gcgttgccgg
    15241 gaaatgggcg tcgataccat tatcgttttc gatacccact ggctggtcaa cagtgcttat
    15301 cacatcaact gtgcagacca ttttgaaggc gtctacacca gcaacgagct gccgcatttt
    15361 atccgcgata tgacctacaa ctacgagggc aacccggagt tggggcagct tattgccgat
    15421 gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca ttcccagcct gaaactggaa
    15481 tacggcacgc tggtgccaat gcgctacatg aatgaagaca agcacttcaa agtggtttcc
    15541 atttcagctt tctgcactgt tcacgatttt gccgacagcc gcaagctggg cgaggcgatt
    15601 ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg ttcgttatcg
    15661 caccgcttta ttgacgatca gcgagcggaa gaagggatga acagctacac ccgcgagttc
    15721 gaccgccaga tggacgagcg tgtggtgaag ctgtggcgcg aaggccagtt caaagagttc
    15781 tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat gcacgacacg
    15841 gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga gtttatcacc
    15901 gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct tcccgcgtaa
    15961 ggacgtttta tgccgcactt tatcgttgaa tgcagtgaca acatccgcga agaagccgac
    16021 ctgccggggt tgttcgccaa agtgaatccg acgctggcag ccacgggtat ttttccgctg
    16081 gcgggtattc gcagccgcgt gcattgggtc gatacctggc agatggccga cgggcagcgt
    16141 gattatgcct tcgtgcatat gacgttgaaa atcggcgcag gtcgcagcct ggaaagccgt
    16201 cagcaggcgg gtgaaatgct gtttgaactg attaaaacgc acttcgccgc cctgatggag
    16261 agccgcctgc tggcgttgtc gtttgagatt gaagagctgc atccgacgct gaattttaaa
    16321 caaaacaacg tgcacgcatt gtttaagtag cgcgcagatt gcccggtggc gctgcgctta
    16381 ccgggcctac aaaaccccaa accgtacacc gtaggccgga taaggcgcag ccgcatccgg
    16441 caatgccaca ggatatcgct atgttcgata aacataccca caccctgatc gcccagcgtc
    16501 tggatcaggc agaaaaacag cgcgaacaga tccgcgcgat ctcgctggat tacccggaga
    16561 tcaccatcga agacgcttac gcggtgcagc gtgaatgggt tcgcctgaaa atagccgaag
    16621 gtcgcacgct gaaaggccac aaaatcggcc tgacctcgaa agcgatgcag gccagctcac
    16681 aaatcagcga gccggattac ggtgccctgc tggatgacat gttcttccac gatggcagcg
    16741 atatcccgac cgatcgcttt atcgtgccgc gcattgaagt ggagctggct ttcgtgctgg
    16801 caaaaccgct gcgtgggcca aactgcacgc tgttcgacgt ctacaacgcc acggactacg
    16861 tgatcccggc gctggagctg atcgacgctc gctgccacaa catcgatccg gaaacccagc
    16921 gcccgcgcaa agtgttcgac accatttctg ataacgccgc caacgccggg gtgatcctcg
    16981 gtggtcgtcc cattaagccc gatgagttgg atctgcgttg gatctccgcc ctgatgtatc
    17041 gcaatggcgt gattgaagaa accggcgtcg ccgctggtgt gctaaatcat ccggcaaacg
    17101 gcgtggcctg gctggcgaac aaactcgcac cgtatgacgt acaactggaa gccgggcaaa
    17161 tcattctcgg cggttcgttc acccgcccgg ttccggcgcg taagggcgac accttccacg
    17221 tcgattacgg caacatgggc tccattagct gccgctttgt ttaaggagag aacgatggaa
    17281 aacagtttta aagcggcgct gaaagcaggc cgcccgcaga tcggattatg gctggggctg
    17341 agtagcagct acagcgcaga gttactggcc ggagcaggat tcgactggtt gttgatcgac
    17401 ggtgagcacg cgccgaataa cgtgcaaacc gtgctcaccc agctacaggc gattgcgccc
    17461 taccccagcc agccggtagt acgtccgtcg tggaacgatc cggtgcaaat caaacaactg
    17521 ctggacgtcg gcacacaaac cttgctggtg ccgatggtac aaaacgctga cgaagcccgt
    17581 gaagcggtac gcgccacccg ttatcccccc gccggtattc gcggtgtggg cagtgcgctg
    17641 gcccgcgcct cgcgctggaa tcgtattcct gattacctgc aaaaagccaa cgatcaaatg
    17701 tgcgtgctgg tgcagatcga aacgcgtgag gcaatgaaga acttaccgca gattctggac
    17761 gtggaaggcg tcgacggcgt gtttatcggc ccggcggatc tgagcgccga tatgggttat
    17821 gccggtaatc cgcagcaccc ggaagtacag gccgccattg agcaggcgat cgtgcagatc
    17881 cgtgaatcgg gcaaagcgcc ggggatcctg atcgccaatg agcaactggc aaaacgctat
    17941 ctggaactgg gcgcgctgtt tgtcgccgtc ggcgttgaca ccaccctgct cgcccgcgcc
    18001 gctgaagcgc tggcagcacg gtttggtgcg caggctaccg ccgtgaagcc cggcgtgtat
    18061 taatgccgga tgcgctgcgc ttatccggcc tacactcgca ccaaatcgta ggccggataa
    18121 ggcgttacgc cgcatccagc aaaaaccttg tcgtacccta caaaaatccc attagaggaa
    18181 gaaaaatgag cgacacctca cctgccatac cggagagtat cgatccggcg aatcagcata
    18241 aagcgctgac tgccggacaa caggcggtta ttaagaagct atttcgccgc ctgatcgtct
    18301 ttctgttcgt gctgtttatc ttctcgttcc ttgatcgcat caacatcggc tttgccggac
    18361 tcacgatggg acgcgacctc ggtctgagcg ccaccatgtt tggcctcgct accaccctgt
    18421 tctacgccgc ttatgtcatc ttcggcattc ccagcaacat tatgctgagt attgtcggtg
    18481 cgcggcgctg gatcgccacc atcatggtgc tttggggcat cgcctctact gccaccatgt
    18541 ttgccactgg ccccaccagc ttgtacgtac tgcgtatact ggttggcatt accgaagccg
    18601 gctttctgcc tggcattctg ctgtatttaa ccttctggtt tccagcctat ttccgcgccc
    18661 gtgccaacgc cttgtttatg gttgcaatgc cggtaacgac agcgttggga tcgctcgttt
    18721 ccggctacat tttgtcgctg gatggcgtaa tggcattaaa aggctggcag tggctgtttt
    18781 tgctggaagg cttcccgtcg gtattactcg gcgtcatggt gtggttctgg cttgatgact
    18841 caccggacaa agctaagtgg ctgacgaaag aagacaaaaa atgcctgcaa gagatgatgg
    18901 ataacgatcg tctgacgctg gttcagccag agggagccat cagccaccac gccatgcaac
    18961 aacgcagcat gtggcgggag atcttcactc cggtggtgat gatgtatacc ctggcgtatt
    19021 tctgcctgac caacacactt agtgcgatca gcatctggac accgcagatc ctgcaaagct
    19081 ttaatcaggg cagcagtaat atcaccatcg gcctgctggc cgccgtaccg cagatttgta
    19141 ccattctcgg gatgatctac tggagccgtc actcagatcg ccgccaggaa cgaaggcatc
    19201 acaccgccct tccttatttg ttcgctgccg cgggttggtt actggcttcg gcaactgatc
    19261 acaacatgat ccagatgctg gggatcatta tggcttcgac cggatcattc agcgcaatgg
    19321 cgattttctg gacaacaccg gatcagtcca tcagcctgcg ggcacgagcg atcggtattg
    19381 cggtgatcaa cgccactggc aacattggtt cagcgttaag tccgtttatg atcggctggt
    19441 tgaaagatct gaccggcagc tttaacagtg gattgtggtt tgttgccacg ctgctggtga
    19501 ttggtgcggg gattatctgg gcgattccaa tgcagtcctc ccgtccgcga gcgaccccgt
    19561 aaggaacgac gatgtgtgac cgtcagattg ccaatattga tatcagcaaa gagtacgatg
    19621 aaagcctggg cacggacgat gtgcattatc agtcgttcgc ccgcatggcg gcattttttg
    19681 gccgccatat gctgccacat cgccacgaac agtactttca gatgcatttc ctcaatagcg
    19741 gacagattga gctacagctt gacgatcatc gctactcggt ggaagcgccc ctgtttgtcc
    19801 tgacgccacc gtcagtacct catgcgttta ttacggagtc tgacgccgac ggtcatgtgt
    19861 tgacggtacg ggaagatctg atctggcccc tgctggaagt tctttatccg ggcactcggg
    19921 aaaccttcgg cctgccgggg atttgcctgt cactggcaga taaacccgac gaactggcgg
    19981 cgctggaaca ctattggcaa ctgatagagc gggaatcggt ggaacaactg cctggacggg
    20041 aacacaccct gacgttactg gcacaggcag tgttcaccct actgctgcgt aacgcaaaac
    20101 tcgacgacca tgccgccagc gggatgcgcg gagaattaaa actgttccag cgttttaata
    20161 tgctgattga aagccatttt catcagcact ggacagtacc ggattacgct aacgaactgc
    20221 atatcaccga atcacgactc acggacatct gccgtcgctt tgccaaccgt ccgccaaaac
    20281 ggttgatttt cgacaggcag ctgcgagaag ccaagcggct gctgctgttt tctgataacg
    20341 ccgtgaacaa tattgcctgg caactcggtt ttaaggatcc ggcttatttt gcgcgctttt
    20401 ttaatcgctt agtcggttgc tcgcccagtg cttatcgcgc caaaaaagta cctgtgacgt
    20461 gaaaatccct tagccttaac gggaaaccag gcaccacctg ctattcccct ttctccgcct
    20521 ggagcaaggg gatttagtaa cgtcaaaaat cgctaaaagc gacctcgatc acaaaaccgt
    20581 acccgacctg aaaagtacca gccattgtcc caaaagtctc ttcccgtaat cgcccctgca
    20641 agcttcaatt gaataacaaa aacaacacac aaaatcaaca attgtattcc gattaatact
    20701 gtagaggtcg acatgaaacc agaagatttc cgcgccagta cccaacgtcc gttcaccggg
    20761 gaagagtatc tgaaaagcct gcaggatggt cgcgagatct atatctatgg cgagcgagtg
    20821 aaagacgtca ctactcatcc ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac
    20881 gacgcgctgc acaaaccgga gatgcaggac tctctgtgct ggaacaccga caccggcagc
    20941 ggcggctata cccataaatt cttccgcgtg gcgaaaagtg ccgacgacct gcgccagcaa
    21001 cgcgacgcca tcgctgagtg gtcacgcctg agctatggct ggatgggccg taccccagac
    21061 tacaaagctg ctttcggttg cgcactgggc gcgaatccgg gcttttacgg tcagttcgag
    21121 cagaacgccc gtaactggta cacccgtatt caggaaactg gcctctactt taaccacgcg
    21181 attgttaacc caccgatcga tcgtcatttg ccgaccgata aagtaaaaga cgtttacatc
    21241 aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc
    21301 aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa
    21361 aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc
    21421 tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc
    21481 tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtgct gatcccatgg
    21541 gaaaacgtac tgatctaccg cgatttcgat cgctgccgtc gctggacaat ggaaggcggt
    21601 tttgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaatt agacttcatt
    21661 acagcactgc tgaaaaaatc tctcgaatgt accggcaccc tggagttccg tggtgtacag
    21721 gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg
    21781 tgttctgaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa
    21841 acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat cgaacgcaac
    21901 gttaccagtg gcctgatcta tctcccttcc agtgcccgtg acctgaacaa tccgcagatc
    21961 gaccagtatc tggcgaagta tgtgcgcggt tcgaatggta tggatcacgt ccagcgcatc
    22021 aagatcctca aactgatgtg ggacgccatt ggcagcgagt ttggtggtcg ccacgaactg
    22081 tatgaaatca actactccgg tagccaggat gagattcgcc tacagtgtct gcgccaggca
    22141 caaagctccg gcagtatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac
    22201 gaccagaacg gctggactgt gccgcacttg cacaacaacg acgatatcaa catgctggat
    22261 aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacag cgcctgcgct
    22321 ttcgtgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg
    22381 ccggacaatg cgggattacg gcaacggccg tctgctcggt cacggataca ccaccatcgc
    22441 tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggtaagt
    22501 tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc acgccacttc gcgggcatga
    22561 caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc
    22621 agccggtgct gaaaggttcg ctggccagtc ttgaaggtga gatccgcgat gtgcaggcaa
    22681 ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc
    22741 acggacttat ctactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga
    22801 tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca
    22861 catatccacc cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttacg
    22921 tccctaaaat aaccactcag ttatttacct tactttacgc gcgcgtaact ctggcaacat
    22981 cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg
    23041 gcctatgatt aacaaattac tctatgaatt ttggtttgca ggaaggcagc aagtgagtga
    23101 atccccggga gcttacaaca gtaagtgaca ggggtgaacg aacgcaactg ccgcacctgt
    23161 aagctaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa
    23221 gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt
    23281 acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct
    23341 ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac
    23401 gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta
    23461 cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg tcggtccggt
    23521 tctcgccgca cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtact
    23581 ggccggtgcg gttcaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc
    23641 tctcggtgag atgataaaag aagagatggg gccagttccg gggactatcg cgctgtttgg
    23701 ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct
    23761 ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat
    23821 gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg tcattggtat
    23881 cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg
    23941 tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt
    24001 ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct
    24061 gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa
    24121 aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt
    24181 cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc
    24241 ttccggtacg acgccaaaac tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg
    24301 cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt ccatcatcga
    24361 accgggtctt tacttcgcga tgaatacccc gcctgctggc cttggcatca ccatgcctaa
    24421 cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac
    24481 cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct
    24541 gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccaac
    24601 gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt
    24661 ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg
    24721 tactcgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa
    24781 aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg
    24841 ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct
    24901 gttcggtatt tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct
    24961 gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct
    25021 tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg
    25081 cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc
    25141 gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag
    25201 tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggctgaa
    25261 tgtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg
    25321 cggcgtgaag atctcttcgc accactaacc gtatttagcc ccgcttcggc ggggctttgt
    25381 tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat ctcggccagg
    25441 cggcgaagat gttgattggc attccagact acgacaacta cgtcgagcat atgaagacca
    25501 accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc cagaacgcac
    25561 gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag catgaacccg
    25621 attgcagtta ccctactcac cggtttttta ggcgcgggca aaaccaccct gctgcgccat
    25681 attcttaatg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt cggcgaagtc
    25741 tccgttgatg atcaattgat tggtgatcgc gcaacgcaga tcaaaacgct gaccaacggc
    25801 tgcatctgct gttcgcgctc caacgagctg gaggacgcgc tactcgacct gctggataat
    25861 ctcgacaagg gcaatattca gttcgatcgt ctggtcattg aatgcaccgg catggccgat
    25921 cctggcccga ttattcaaac ctttttctcc catgagattt tatgccagcg ctacctgctg
    25981 gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa ccagttcacc
    26041 atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac cgacgtcgca
    26101 ggcgaagcag aaaaactgcg tgaacgcctg gcgcgcatca acgcccgtgc accggtctac
    26161 accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg ttttatgctg
    26221 gaagaaaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa acaaaacgat
    26281 atttcgtcga ttgtggtgga actggattac ccggtagata tcagcgaagt ttcccgggtg
    26341 atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg gatgctgtgg
    26401 attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta cagcgccgac
    26461 tgggacaggc catggggcga tgaaacaccg catagcacga tggtgtttat cgggattcag
    26521 ttgccggagg atgagattcg ggcggcgttt gcgggactca aaaagtaacc agccctccaa
    26581 catcacaata cctgagactt tatcaccctc atattcctcc agaatatgag ggctttcggg
    26641 cttaattatc tctgaacgtg caacacttcc tgcgactcct tatgcgcctc cacatcctgc
    26701 ttcgttaacc agagcgactt acggccaaaa ttttcgtaca ttttcagctg atcttcatag
    26761 tgcttatcaa ctgttccatc gggagcaata aacccactct gaccgggtgc gaccacatcc
    26821 caggcaagca caggacgatc gcttgtcgtt ggtgagaaaa caatcatatc gttttctgtt
    26881 ccacggtttt gatactccgc ctgatgacgc gtttcttccg ctgcggcctg cggtacacca
    26941 aagaaattat ttgcccggaa cgttaaggcc attgcaggtg ttttccagtt actcacatta
    27001 ttgccatagc gtttggaaag agtctcccag gtatcttcca gcgcagccaa cacaacctcc
    27061 tgctgtggtt tcccagcaaa cagatcaacc gcctgtggga ttggtgattt gtctccctgc
    27121 accgcctcat acaaaatttt tgctccaaca cttatattca gcgaaccagt tgggccgtcc
    27181 tgggttgttt cgtagccact ggcgctgtac cacttatcaa atggcatagg tacggcagcc
    27241 actacggtac gcttcaacat actggtcagc caaacgttca ggatggcaga gcctggctgc
    27301 tgccaggttt taccatcatc attaagcaaa ttgatgccat cccaacgtgt taatgtttct
    27361 accaactgac gacgcggatc gctctgtgtc aaaccagatg tcgctgcttg cagagtaggt
    27421 aaaaaaagcc tcaggttaag atcctgacga ctggtttggc gaataacatc ccatgcctga
    27481 tcagcagtta agcgtggctt ttgctcaagc agtcggtcga tctccgtaac gcgatctgca
    27541 ccaccccaca aaaaggcaaa cagatctgaa gcgggataat ctttttgggg agaattgttc
    27601 cagttagcaa tatatcccga ctgggggtta tacaccttag ggttcatttc aaaaggcaat
    27661 agccctttcc agtcccattt tcccgtacca ggaacgggta atcgcggatc atggcctgat
    27721 tgacgatctg gataagcacc agtatgaaca taaccaatat tgccgtttac atcagcatag
    27781 taccagttga tggtcagtgc ttgtttcgct gcctgctgtg tccactcctg ccaatttttg
    27841 gccttcatct gatgagtcca ggccagcaaa gacgccacct ctttaccatc ccatgcgcgg
    27901 gatttagcgt aagccgtttg tgtcgtctgg tcagtttgga gaatgttgcc atgcaccgta
    27961 cgccagacag taaaggtctc tgcctgacca tttttcaccg taatggtttc ctcacggctt
    28021 aacattttca cccacttacc attatgcaag tagtagcctg gtttctctgc cgacagccgt
    28081 tcagcaaaaa tatcgacatc atcgccgaaa cctgccgttg atccccagga aatcacacca
    28141 ttatgaccaa aaaccagccc aggataggca aatggtgtat tgccagtgac atcataacca
    28201 gcaccgtgca gaccaatacc ataagtatac gcaggcgcat accagccaaa ctgcggacca
    28261 tttaccatga ttgctttcgc atcctgggct ttgcttttgc cgatcaccca catattgctg
    28321 gtcgttggat accccgccag accattggca ccaccctgtg caaattgtgc agcaatagtt
    28381 tcccggttct tccctgctgt taacgccagc agtgcgccat ccgccccttt tgctggtcgg
    28441 tcaagcattg gtgcaggtaa atcgtagcgt ggcaacagag ctgctgtttg cgagttttgc
    28501 tgattaaatt taagtgggta gttactctct tgtacggcaa tagtggttgg cgctgatggg
    28561 tttaccagcc atttcaactg attaaatacc gccatgcctt gtgatacacc atatttatct
    28621 tttaaagccg ttagcagtgc cagattatca atttcgctag tgctatcaga gaagcggttt
    28681 gccatggtgc ccacaaatat catcgcgaca tcaaacggtt cccagcgctt aggagtaaag
    28741 ccaaatgtat taaactgttt tggtaagagc gtctctggat tggtatttac cttatcaatc
    28801 caggcattca ttccatcagc gtagccttgc agaatggaca tatcctctgg ggaaagggca
    28861 gcaatttgcg cccggatagc atccggccag tagttacgac ggatatcttt atcaaatttc
    28921 acaaaatctt tgccaagcac ttccgcgaca gtcccttgag tactgcgacg tgccatttcc
    28981 atctgaaaaa ggcgatcttg tgctactaca tagccatagc cataaaatag gtgccatgta
    29041 tcattggcat aaatatgcgg catgccgtat tcatcgcgaa caatctttat ctcacttgac
    29101 gactgctcag ccagtgcagg taagctccaa taatacatca gggaagcagt aacacagttc
    29161 acgatcatac gatttctatt tttcattgta tcctctggca ggtgtataat tagcgaattg
    29221 atactagcaa cgaagcttcg gttatctatt tgcagagaat tgtttcatta tgaactgtga
    29281 gcgcgataac taatttttga tacaaaaatt tcattaatgt gatctaaaac gaatcgcttt
    29341 attcaccgct gatgattttg ttctattcct ttttgtgaca gatcccccgg acgcatagat
    29401 gatgaccccc agagagacag ctcaggtcat tatctgtatg taaatcagaa aaatatgttg
    29461 taaacgactt ttatggttgg attttgtagc aattttgaat gcagacaggc agcatttgcc
    29521 aacacgccgc ccatagttca ctccacatcc tccctgcgta acccaatatc cctcaaccgt
    29581 tcatcactca tctgctgtaa cactcgtcgg gtctgcattt gccgccgcca acgcctcacc
    29641 gcatgccaga gttgcacaag tctgataaac ggcgttttgg ctttgttttc gtgaaattcc
    29701 atctgctgcc tcctcatctt tcagaggctt catcatccgc cttctggcag ttcacaatac
    29761 agatgcaaat attagtttta tttaacatac agacaggtta aaacagcatc tgcatccggt
    29821 tttttagcct catctgtacc ggtttttctt tctgtatggg ccaaataagg aatacagcat
    29881 gacgcgttat caacatctgg cgactctact tgccgagcgg attgagcaag ggctgtatcg
    29941 tcacggggag aaattgccgt cggtgcgcag cttaagtcag gagcacggcg tcagcatcag
    30001 caccgtgcaa caggcgtatc agacgctgga aacgatgagg ctcatcactc cgcagccgcg
    30061 ttcgggttat tttgtcgcac aacgtaaagc ccagccgcct gtaccgccga tgacgcgtcc
    30121 ggtgcagcgc ccggtggaaa ttacccagtg ggatcaggtg ctggatatgc tggaggcgca
    30181 tagcgacaat tccattgttc cgttaagcaa aagcacgccg gatgtcgaaa cgcccagcct
    30241 gaaaccgctg tggcgtgagc taagccgggt ggtgcagcat aatctgcaaa ccgttctcgg
    30301 ttatgacttg ttagccggtc agcgagtgtt gcgggagcag attgcccgcc tgatgctcga
    30361 cagcggctcg gtggtcaccg ccgatgacat catcatcacc agcggctgcc ataactcgat
    30421 gtcgctggcg ttaatggcgg tgtgtaaacc gggcgatatt gtcgcggtcg aatctccctg
    30481 ttattacggt tcgatgcaga tgctgcgagg catgggcgtg aaagtgattg aaatcccaac
    30541 cgatccagaa actggcatca gcgttgaagc actggaactg gcgctggaac agtggccgat
    30601 taaaggcatc attctggtgc caaactgtaa taatccgctg ggatttatta tgccggacgc
    30661 acgcaaacgg gccgttctct ctctcgctca gcgtcatgat attgtgattt ttgaagatga
    30721 tgtctacggc gaactggcaa cggagtatcc gcgcccgcgg accatccatt catgggatat
    30781 cgacgggcga gtgctgttgt gcagctcgtt cagtaaaagt attgctccag gcctgcgcgt
    30841 gggttgggtc gcaccggggc gttatcacga taaactgatg catatgaaat acgccatcag
    30901 cagctttaat gtgccgtcca cgcaaatggc ggcggcaacg tttgtgttgg aaggccacta
    30961 tcatcgccat atccggcgga tgcggcagac ttatcagcgc aatctggcgc tttatacctg
    31021 ctggatacgg gaatattttc cctgcgaaat ctgtattacg cgcccgaaag gcggattttt
    31081 actgtggata gaattgcctg aacaggtcga tatggtctgc gttgcgcggc agctgtgccg
    31141 catgaatatc caggtggcag caggctcgat tttctcggct tccggcaaat accgtaattg
    31201 tctacgcatc aactgcgctt tgccgctcag cgaaacctat cgcgaagcac taaagcaaat
    31261 tggcgaggcc gtgtatcggg caatggaata aattcgaatt agtggcgaag cacctgattt
    31321 cctctcacga atcgcctgta actcgttgta tctttgcagg gtcatcgcca cgttccagca
    31381 tggcttaggc aaagtgtgct tcgttcatac cggatgcggc gtgaacgcct tatccggcct
    31441 acaaaccgcg caaattcaat agattgcaga ggaaatgtag gtctgataag cgtagcgcat
    31501 caggcagctt tgcgtttgtc atccgtctct gattagtatt cgatccatgg atcgcgcttg
    31561 atttcaggca agggctgctc taatgcctgc atttgctctg tcgaaagtcc ggtaatcttt
    31621 tgtatccatt caggatctgc cccattctgc aacaacaatc taaggaggcg ttgttcccct
    31681 tcttctttcc ccttttccat ccccaacagc catccatcat tataaattcg ctctgcaatc
    31741 gtcattattc gctccctgtg ttgtggcatt cgacgggtaa gttcgctgat aaacgcctta
    31801 aaacgcgctt catcgccagt cagtaaaatg taatttaaca gcgcagttat ctggctgtca
    31861 ttagcacact ctgtaaccag taatactacc agttggtcaa taagccccat cagatcgcgc
    31921 tggcgaatat gcttttggat caactccaac agggcgactc tgcgatgctg cacaatctcg
    31981 tcgtctggca cgacagtgac atccaccagc gggaacgctg cgctataaag tttccgtgcg
    32041 gtagtcgggg cggcaaattc gtccagccag cacagggacc aggggtaagg actacggcta
    32101 ccgtgataaa acagcatcgg gataaccagc ggtagcggcc ggcgtttatc atgctcgata
    32161 tggcgctgca tcaccgccat ggaatatcgc atcaggcgaa acgccatatg aatatcctcg
    32221 cggctctgat gttcaatcac tacgtaaata taaccatcac cttcacgggt ctttaccgac
    32281 cacagaatat cggagtgtag cgcccgcaat ttttcatcga caaagctggc ggattccagt
    32341 tttaagctgt cgagatcgca cagttcacgt aaatctttgg gtaagtggat ctccataaaa
    32401 tcccgcgcgg tgtcagggtg agtgagaaag gatttaaata atgcgtcatg tggcgtgctg
    32461 gtcgtgaagt ttgtcatggc gatccgtcac ctgtagataa gatggcccga ctttagcgac
    32521 taacaaaacc attctcaccc ggcaatttct cgttccccga ggtgctttcc aggctatttt
    32581 tgttcgtctg caaacgatac atttttccgg aaacaggctc gcgttttaca tttcctgtac
    32641 ccggcagata ttcccctttc caccgcgctc ttttgattaa ccgcaaattt taattacact
    32701 taaggtgtat attttctatg caacccatca attaaagagg tgtaatgtgc tgatgactat
    32761 ttgtaatcgt tatacattct gacctggagt cagaaggtat ttctctgtct gtgtgttcac
    32821 aggcagtgtg gttgattaca tgaaattcag tacatttgtc cttctcgcct gcctttcgtc
    32881 attaccgacg gtattgaatt tcgttttccc cgttggggtt ctccggacaa ggagttgttt
    32941 gttatgccac gtttttttgc ccgccatgcc gccacgctgt ttttcccgat ggcgttgatt
    33001 ttgtatgact ttgccgcgta cctgtcgacg gatctgatcc agccggggat cattaatgtg
    33061 gtccgtgatt ttaatgccga tgtcagtctg gccccggcag ccgtcagtct ctatctcgcg
    33121 ggcggtatgg cgttacagtg gctgctgggg ccgctttccg acaggattgg ccgcaggccg
    33181 gtgctgatta ccggggcgct aatatttacc cttgcctgcg ctgcgacaat gttcacaacg
    33241 tctatgacac agtttcttat cgcgcgtgca attcagggca ccagtatctg ttttattgcc
    33301 accgttggtt atgtcacggt gcaggaggcg ttcggccaga caaaagggat caagttgatg
    33361 gcgattatca cctccatcgt actgattgcg ccgattattg gcccgctttc cggcgcagct
    33421 ctgatgcact ttgtgcactg gaaagtcctt tttgccatca ttgcagttat gggttttatc
    33481 tcatttgttg gcttactgct ggcgatgcca gagacggtga agcgcggcgc ggttccattt
    33541 agcgccaaaa gcgtctttcg cgattttcgt aatgtctttt gcaatcggct gttcctcttt
    33601 ggcgcagcaa ccatctcttt aagctatatc ccaatgatga gttgggtggc tgtctcgccg
    33661 gtgatcctga tcgacgcggg cggcttaaca acttcgcagt tcgcctggac acaggttccg
    33721 gtgttcggcg cggtgattgt cgcgaatgcc atcgtggcgc gtttcgttaa agatccgacc
    33781 gaaccgcgct ttatctggcg tgctgtaccc attcaactgg tcggcctcgc gctgttgatt
    33841 atcggcaatc tgctgtcacc gcacgtctgg ctgtggtcgg tgctgggcac cagtttgtat
    33901 gctttcggga ttggtttgat cttcccgacc ttattccgct ttacgctttt ttccaataac
    33961 ttaccgaaag ggaccgtctc cgcatcgctg aatatggtga tcctgatggt gatgtctgtc
    34021 tcggtcgaaa tcggccgctg gctgtggttt aacggcggtc gcttgccgtt tcatctgtta
    34081 gccgtcgtgg cgggcgttat cgtcgttttc accctggcag gattgctcaa tcgcgtgcgc
    34141 caacatcagg ctgccgagct ggcggaggag cggtgatttt tgcgcgatcc tgccgtcagg
    34201 ctctattctt aacgttatga ataaactcat tgaactcaga cgcgccaaaa tgttggcgct
    34261 ctctttactg cttatcgccg ctgctacctt tgtcgttacg ctgtttttgc cgcccaattt
    34321 ttgggtgagc ggcgtgaagg cgattgctga agcggcgatg gtcggcgcgc tggcggactg
    34381 gtttgcggtg gtggcgctgt ttcgccgcgt gccgattccg atcatttctc gtcatacggc
    34441 gattatcccg cgtaataaag accggattgg cgaaaatctc ggccagttcg tgcaggaaaa
    34501 atttctcgat acccagtcgc tggtggcatt gattcgacgc cacgaaccgg tgttgttgat
    34561 tggcaactgg tttagtcagc cagaaaacgc ccgccgcgtt ggtcagcatc tgttgcagat
    34621 catgagcggt tttcttgaac tgaccgatga tgcgcgtatt cagcgcctgc ttaagcgcgc
    34681 ggtccatcgg gcgattgata aggtcgatct ttccggcacc agtgcgttga tgctggagag
    34741 tatgaccaaa aacgatcgtc atcaggtgct actggatacg ctgatcgcac agttgatcgc
    34801 ccttctccag cgcgataaat cgcgcaagtt tattgcccag caaattgttc gctggctgga
    34861 gagtgagcat ccactgaaag ccaaaattct ccccaccgaa tggttgggcg aacatagcgc
    34921 ggagttggtt tctgacgcgg tgaattcttt gcttgatgat atcagccgtg atcgtgcgca
    34981 tcagatccgt catgcgtttg atcgcgccac ttttgccctg atcgacaagt tgaaaaacga
    35041 tccggaaatg gcagcgcgag ccgatgccgt aaaaagttat ctgaaagaag atgaagcttt
    35101 taaccgctat ctcagtgaat tgtgggggga tttacgggag tggctgaaag cggatatcaa
    35161 cagtgaagat tctcgtgtga aagaacgtat cgcgcgggcg ggtcaatggt ttggcgaaac
    35221 gttaattgcc gatgatgcct tgcgggcgtc gttaaatggt cacctggaac aagccgcgca
    35281 ccgcgtcgcg cctgagtttt ccgcattcct gacgcgccac atcagcgata cagtaaaaag
    35341 ctgggatgcg cgggatatgt cgcggcaaat cgagttaaat atcggcaaag atctgcagtt
    35401 tatccgtgtc aacggtacgc tggttggcgg ttgtattggg ctaattttat atttgttgtc
    35461 gcagctcccg gccttgttcc ccctcagcaa tttttagaaa tccatgaaaa actaagtggc
    35521 taaccgcgag agagatcaaa taaaccacac taaaagaatg gtaataatgt ggcctctttt
    35581 cattatctcc cgtggtacgg ggaaggaaaa tcatgtcact tatcaccgat ctacccgcca
    35641 ttttcgatca gttctctgaa gctcgccaga aaggctttct caccgtcatg gatctcaagg
    35701 agcgcggcat tccgctggtt ggcacttact gcacctttat gccgcaagag atcccgatgg
    35761 cagccggtgc ggttgtggtt tcgctctgtt ctacctctga tgaaaccatt gaagaagcgg
    35821 agaaagatct gccgcgcaac ctctgcccgc tgattaaaag cagctacggc tttggcaaaa
    35881 ccgataaatg cccctacttc tacttttcgg atctggtggt cggtgaaacc acctgcgacg
    35941 gcaaaaagaa aatgtatgaa tacatggcgg agtttaagcc cgttcatgtg atgcagttgc
    36001 cgaacagcgt taaggacgat gcctcgcgtg cgttatggaa agccgagatg ctgcgcttgc
    36061 aaaaaacggt agaagaacgt tttgggcacg agattagcga agatgctctg cgcgatgcca
    36121 ttgcgctgaa aaaccgcgaa cgtcgcgcac tggctaattt ttatcatctt gggcagttaa
    36181 atcctccggc gcttagcggc agcgacattc tgaaagtggt ctacggcgcg accttccggt
    36241 tcgataaaga ggcgttgatc aatgaactgg acgcgatgac agcccgcgtt cgtcagcagt
    36301 gggaagaagg ccagcggctg gccctgcgtc cacgcatttt aatcaccggc tgcccgattg
    36361 gcggcgcagc agaaaaagtg gtgcgcgcga ttgaagagaa tggcggctgg gttgtcggtt
    36421 atgaaaactg caccggggcg aaagcgaccg agcaatgcgt ggcagaaacg ggcgatgtct
    36481 acgacgcact ggcggataaa tatctggcga ttggctgctc ctgtgtttcg ccgaacgatc
    36541 agcgcctgca aatgctcagc cagatggtgg aagaatatca ggtcgatggc gtagttgatg
    36601 tgattttgca ggcgtgccat acctacgcgg tggaatcgct ggcgattaaa cgtcatgtgc
    36661 gtcagcagca caacattcct tatatcgcta ttgaaacaga ctactccacc tcagatgtcg
    36721 ggcagctcag tacccgtgtc gcggccttta ttgagatgct gtaaggagtg gcagtgacat
    36781 attcgattgg cattgattct ggttctaccg ccaccaaagg gatcttactg gcagacggcg
    36841 tgattacgcg ccgtttcctc gttccaacac cctttcgtcc ggcaacagca attactgaag
    36901 cctgggaaac tctgcgcgaa gggttagaga caacgccgtt tctgacgctc accggctatg
    36961 gacggcaact ggtggatttt gccgataaac aagtgacgga aatctcctgt catgggctgg
    37021 gcgcacggtt tcttgcgcca gcaacgcgcg cggtaatcga catcggtggt caggacagca
    37081 aagtgattca gcttgatgat gacggtaacc tgtgcgattt cctgatgaat gacaaatgcg
    37141 cggcgggcac cgggcgtttc ctggaggtga tctcgcgcac gcttggcacc agcgtcgagc
    37201 aactcgacag cattaccgaa aatgtcacgc cgcacgccat cacgagtatg tgcacagtgt
    37261 ttgccgaatc agaagtgatc agcctgcgct cagcgggcgt cgcgccagaa gcgattctcg
    37321 ccggagtgat taacgcgatg gcgcggagga gtgccaattt cattgctcgt ctctcctgtg
    37381 aagcgccgat tctgtttact ggtggcgtta gtcattgcca gacgtttgcc cggatgctgg
    37441 aatctcatct gggaatgccg gtaaataccc atcctgatgc gcaatttgct ggcgcaattg
    37501 gcgcggcggt aattggtcaa cgagtgagga cacaccgatg aaagagtttt tatttttatt
    37561 tcactccacg gtcggcgtca tacaaacccg caaggcgttg caggcagcgg gcatgacttt
    37621 tcgcgtcagc gatattcccc gtgatttacg cggcggctgc gggttatgca tttggctgac
    37681 ctgtcccccc ggcgaggaaa tacaatgggt gatccctggg cagactgagt caatttattg
    37741 ccagcaggat ggtgtctggc gctgcattgc acattacggg gtttctccgc gttaaggatt
    37801 cattaaggtc gctacgtata cttctcgata tcttattgat ttgccttaaa gggattaccg
    37861 tgacaaaaag tatttcgtta agtaaacgca tctccgttat tgtgattctt tttgccattg
    37921 tggcagtgtg tactttcttc gtgcaatctt gcgcccgtaa aagcaatcat gcagcatcat
    37981 ttcagaatta ccacgccacc attgacggca aagaaattgc aggcataacg aataatatct
    38041 cttcgctgac ctggtcggca caaagcaata ctctttttag cactatcaac aaacctgccg
    38101 ccattgttga aatgaccacc aatggtgatt ttatccgcac aattcctctg gattttgtta
    38161 aggatctcga gacgatcgaa tacattggcg ataatcagtt tgtcattagc gatgagcgcg
    38221 attacgccat ttatgtgatt tcactgaccc caaactcgga agtaaaaatc ctcaaaaaaa
    38281 taaaaatccc gctgcaagac tccccaacca attgcggttt tgaagggttg gcttattctc
    38341 gccaggatca cactttctgg ttttttaaag agaaaaatcc gattgaggtc tacaaagtta
    38401 acgggctgtt aagtagcaat gaattacaca tcagcaaaga cgaggcacta caacggcaat
    38461 ttactttaga tgatgtgtcc ggcgcagaat ttaaccagca aaaaaatacg cttctggtgc
    38521 tatctcatga atctcgcgca ctccaggaag taaccctggt tggcgaggtt atcggtgaaa
    38581 tatcactaac caaaggtagt cgaggtttgt cgcataacat caaacaggcc gaagggattg
    38641 cgatggatgc ctctggtaat atttatatcg ttagcgaacc aaatcgtttt tatcgcttta
    38701 cccctaaatc atcgcattaa caggctggca tatttcacct ttttgttatt aacttgttaa
    38761 caacaaaaat gacatacaag gttaaacatg ccttcgtcct cgcatcccgt agaacgcttt
    38821 tctttcagca ccgcgctttt cgggatgctg gttctgacct taggtatggg attaggtcgc
    38881 ttcctttata cgcctatgtt gcccgtcatg atggcggaag gggcgttttc atttagccag
    38941 ctctcgtgga ttgccagcgg caactatgcg gggtatctgg ctggcagtct gctattttcg
    39001 tttggcgcat ttcaccagcc gtcgcgcctg cgcccattac tgttggtttc tgccctggcg
    39061 agcgggttgt tgatcctcgc aatggcatgg ttgccgccat ttattctggt gttattgatt
    39121 cgcttcctgg cgggtgtcgc cagcgccggt atgctgattt ttggttcgac gctgattatg
    39181 cagcacacgc gccatccttt tgtgctggca gctttgtttt ctggcgttgg cattggcatt
    39241 gcactgggca acgaatatgt tctggcaggc ctgcattttg ccttctcctc gcaaacgtta
    39301 tggcaaggcg ccggggcgct ttctggcatg atgctgattg cacttacgct tttaatgccc
    39361 tcgaaaaaac acgccatcgc accaatgcca ttggcaaaaa cggagcaaca gataatgagc
    39421 tggtggttac tggctattct gtacggcctt gcgggatttg gttatatcat cgtcgcgact
    39481 tacctgccgc tcatggcgaa agatgcaggc tcaccattgt taaccgccca tctctggacg
    39541 ttagtcggct tatcaattgt gccaggctgc tttggctggc tatgggcagc aaaacgttgg
    39601 ggggctttgc cttgcctgac ggcgaatttg ctggtgcagg ctatcagtgt gctgcttact
    39661 ctcgccagcg actctcctct cttgcttatc attagtagta tcggttttgg cggtaccttt
    39721 atgggcacga catctctggt gatgactatc gcccgccaac tgagcgttcc gggaaatctc
    39781 aatcttttag gctttgtgac gctcatttac ggcattggac aaatcctcgg cccggcgctg
    39841 accagtatgc tcggcaatgg aacatcggcg cttgccggcg ccactctctg cggcgcggcg
    39901 gcgttgttta ttgctgcgct tatcagcaca gtacaattat ttaagctgca agtggtcact
    39961 tcatgaggag tgtttccctt accactcaag aaactcaaca gcaaccgttg atgtcagcca
    40021 gaccccgttt tccgcttgcc agaaagggat gcctcgtttc gccatttcct gtgctttgac
    40081 ggttaagata accggcgatc cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc
    40141 agagagatgt acgtaatgac gctcccctgc aatcagcccc tgttttttta tctcgtcaag
    40201 gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat
    40261 ggcgacctgc gaagtcgaat gcccctgaac cgcgcggatg cacctaccat cactggaata
    40321 actaaaacgc tttttatcgc tggtggcaac cacagtatcc agcaatgcac gagttaaccg
    40381 cttgcccgct ttttgggcac aaagtatgag cttatcgata tccgcccatc cttcacggtc
    40441 aagtacgata ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt
    40501 atccgccaac tctttttcgt tgtactttgc catcgcgttt tcgtccctgg attttctgtt
    40561 tttatttact gatgtcattg tcactgggtt tatgaatttt ctgcaactca atcctgcgca
    40621 gagccttttc tacacagcgt aatttttccg ctatgcaaaa aacgcatagc aagaaacata
    40681 cttgcatttt ccccataccc acatggctga taaggttttt cagctccagg aattttcctg
    40741 gctcaagcaa cacacaaaca caaaaaaaca atggggattg ttatgacaca acaaggggat
    40801 gctgtagccg gggagctggc gacagaaaaa gtgggcatta aagggtatct ggcttttttt
    40861 ctcaccataa tctttttttc cggagttttt tccgggacag acagctggtg gcgcgttttt
    40921 gatttttccg ttctgaacgg atcgtttggg caactgcctg gtgcgaatgg agccaccacc
    40981 tcatttcgcg gggcgggtgg cgcgggggct aaagatggct ttctctttgc gctggagctg
    41041 gcaccgtcag tcattctctc tctggggatt atttcaatta ccgatggtct tggtggatta
    41101 cgcgccgcgc agcagttaat gacgcctgtg ctaaaaccgt tgttgggcat tccagggatc
    41161 tgctcgctgg cgctgatcgc taacctgcaa aacactgatg ccgccgccgg tatgaccaaa
    41221 gagctggcgc aggaagggga aattaccgaa cgcgataaag tgattttcgc cgcatatcaa
    41281 accagcggta gcgccatcat caccaactac ttctcttccg gcgtcgcggt gttcgccttt
    41341 ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc
    41401 aacattttgc gcgtctggct taacttcgaa gaacgtcgca acccaacgca aggagcgcaa
    41461 gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg
    41521 gctttactat cgccacgacc aacctgctgc caaacgtggt gatggcgttt gtcatcattc
    41581 aggcgctgaa aattaccggc ctgctcgact gggtggggca tatctgtgaa cccgtaatgg
    41641 cgctgtgggg attacccggt gaagcggcaa ccgtcctttt agccgcatta atgagtatgg
    41701 gcggcgcagt cggtgttgcc gccagtctgg cgaccgccgg cgcattaacc ggtcacgatg
    41761 tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct
    41821 gcctggggac agccgaagtg aatgccaaat attacccgca tatcattacg gtctgcgtga
    41881 ttaacgcatt gctgtcgatc tgggttatgc agcttattgt ttaacaagga gttaccatga
    41941 ttgattatac cgcagccggt tttaccctgc tgcagggagc gcatttgtat gcgccggaag
    42001 atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat tatcgccgtt gccagcaata
    42061 tcccttctga cattgtaccg gactgcacgg ttgtcgatct cagcgggcag atcctctgtc
    42121 ctggttttat tgatcaacac gtccatttga ttggtggcgg cggcgaagca ggtcccacga
    42181 cgcgcacgcc agaagtggcg ctaagtcgcc tgacggaagc gggcgttacg tcagtggttg
    42241 gtctgctggg caccgactct atctctcgcc acccggaatc cctgctcgcc aagacccgtg
    42301 cgctcaatga agaaggcatc agcgcctgga tgctgaccgg cgcttatcat gtcccttccc
    42361 gcaccattac aggttccgtg gaaaaagacg tggcgattat cgatcgtgtg attggcgtga
    42421 aatgcgccat ctctgatcac cgttctgccg caccggacgt ttatcacctg gctaatatgg
    42481 cggcggaatc ccgcgttggc ggtttgctcg gcggtaaacc tggcgtcacc gtgttccaca
    42541 tgggcgacag caaaaaggcg ttacagcctg tctatgacct gctggaaaac tgcgatgtgc
    42601 cgatcagcaa gttgctgccg acccacgtta accgcaacgt tccgttgttt gagcaggcgc
    42661 tggagttcgc gcgcaaaggc ggcactatcg atatcaccag cagcattgac gaaccggtcg
    42721 cccctgccga aggtattgcc cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc
    42781 tcagctccga cggcaacggt agccagccgt tcttcgatga cgaagggaat ttaacccata
    42841 tcggtgttgc cggttttgaa acgttgctgg aaaccgtgca ggtgctggtc aaagactatg
    42901 atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag cgtagccggt ttccttaacc
    42961 tgaccgggaa aggcgaaatt ctgccaggca atgatgctga cttgctggtc atgacgccag
    43021 aactgcgcat tgagcaggta tacgctcgcg gcaaactgat ggtcaaagac ggcaaagcct
    43081 gcgtgaatgg aacgtttgaa acggcttaaa cggttgtcag ccgcccgaat ggacgtagaa
    43141 tgcccgcaag ggcggctgac agagtaaaac gtaatggatg actgtggtgc gattttgcat
    43201 aatattgaaa ccaaatggct ttatgatttt ctgaccctgg aaaaatgccg caatttttcc
    43261 caggcggcag tcagtcgcaa cgtctcgcaa ccggcattca gccgccgcat ccgtgcgctg
    43321 gaacaggcga ttggtgttga attgtttaac cgccaggtaa cgccgctgca actctcagaa
    43381 caagggaaaa tcttccattc gcagatccgc catctgttgc aacagttaga gagcaatctg
    43441 gcagagctgc gtggcggcag cgattacgcg caacgaaaaa tcaagatagc cgctgcacac
    43501 tctctttccc tcgggctgtt accgtccatt atcagccaga tgccgccgct ctttacctgg
    43561 gcaattgaag ctattgatgt cgatgaagcg gtcgataaac tgcgtgaagg gcaaagtgac
    43621 tgtatttttt cctttcacga cgaagatttg ctggaagcgc cgtttgacca cattcgctta
    43681 tttgaatctc aattgttccc tgtctgcgcc agtgacgaac acggagaagc actttttaac
    43741 ctcgcgcagc cacactttcc gttactgaat tacagccgca actcctacat ggggcgattg
    43801 attaatcgca ccctgacgcg ccacagtgag ttaagtttca gcaccttttt tgtctcttcg
    43861 atgagcgagc ttttaaagca ggttgccctc gacggctgtg ggattgcctg gctgccggag
    43921 tacgccatcc aacaagaaat tcgcagcggg aagctcgttg tactgaatcg ggacgaactg
    43981 gtgatcccga ttcaggccta tgcataccga atgaataccc gcatgaatcc agttgccgaa
    44041 cgcttctggc gtgaactgcg cgagctggag attgtgctta gctgacattc tccagcatcg
    44101 cactacacca accgccatta tcccagacca gatcaaacgt tagcggttca ccgtcgtggt
    44161 acagcattat ctcaaggctg atcgccagcg tgtggctgta cgcttctttc agattgacct
    44221 gcaacgccgc aatccgtggc tcatgaaata acagtgcaga acgcagctga cggcaatacc
    44281 agctctggag atgtttgtcg ctctggttat gcacttgcca ctcgggcatg cctgaaacaa
    44341 gtaatgacgg taatgccgaa tgaggagaat taaaaagagt gaggagatcg ctacatactg
    44401 atttacgcag cgatgacgtt ttattgcctg aaatttcagg taaaacagcc tgaagtgatt
    44461 ggcgcatcat tttgcgcact cctgacgttt agcaacatat gcggtttgcc ctgaacaccg
    44521 ggcaccactg tattgatgct cattttccat agccattccc ttttgttcat tacatccatg
    44581 tcagtctgag acaggcctga atcctgtcag caaagtataa aaccttactt gcctatgaat
    44641 atctactgac ttttcag
//
LOCUS       DAZFVO020000019        44070 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFVO020000019 DAZFVO020000000
VERSION     DAZFVO020000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 44070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.019"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            483..932
                     /gene="alaE"
                     /locus_tag="ACHKNB_RS04980"
                     /old_locus_tag="pgaptmp_000996"
     CDS             483..932
                     /gene="alaE"
                     /locus_tag="ACHKNB_RS04980"
                     /old_locus_tag="pgaptmp_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HGU4194561.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(969..1313)
                     /gene="ygaC"
                     /locus_tag="ACHKNB_RS04985"
                     /old_locus_tag="pgaptmp_000997"
     CDS             complement(969..1313)
                     /gene="ygaC"
                     /locus_tag="ACHKNB_RS04985"
                     /old_locus_tag="pgaptmp_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HGU4194562.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1465..1794
                     /gene="ygaM"
                     /locus_tag="ACHKNB_RS04990"
                     /old_locus_tag="pgaptmp_000998"
     CDS             1465..1794
                     /gene="ygaM"
                     /locus_tag="ACHKNB_RS04990"
                     /old_locus_tag="pgaptmp_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HGU4194563.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2042..2287
                     /gene="nrdH"
                     /locus_tag="ACHKNB_RS04995"
                     /old_locus_tag="pgaptmp_000999"
     CDS             2042..2287
                     /gene="nrdH"
                     /locus_tag="ACHKNB_RS04995"
                     /old_locus_tag="pgaptmp_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGU4194564.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2284..2694
                     /gene="nrdI"
                     /locus_tag="ACHKNB_RS05000"
                     /old_locus_tag="pgaptmp_001000"
     CDS             2284..2694
                     /gene="nrdI"
                     /locus_tag="ACHKNB_RS05000"
                     /old_locus_tag="pgaptmp_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGU4194565.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2667..4811
                     /gene="nrdE"
                     /locus_tag="ACHKNB_RS05005"
                     /old_locus_tag="pgaptmp_001001"
     CDS             2667..4811
                     /gene="nrdE"
                     /locus_tag="ACHKNB_RS05005"
                     /old_locus_tag="pgaptmp_001001"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4194566.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTYTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4821..5780
                     /gene="nrdF"
                     /locus_tag="ACHKNB_RS05010"
                     /old_locus_tag="pgaptmp_001002"
     CDS             4821..5780
                     /gene="nrdF"
                     /locus_tag="ACHKNB_RS05010"
                     /old_locus_tag="pgaptmp_001002"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4194567.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6136..7338
                     /gene="proV"
                     /locus_tag="ACHKNB_RS05015"
                     /old_locus_tag="pgaptmp_001003"
     CDS             6136..7338
                     /gene="proV"
                     /locus_tag="ACHKNB_RS05015"
                     /old_locus_tag="pgaptmp_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HGU4194568.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGNRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7331..8395
                     /gene="proW"
                     /locus_tag="ACHKNB_RS05020"
                     /old_locus_tag="pgaptmp_001004"
     CDS             7331..8395
                     /gene="proW"
                     /locus_tag="ACHKNB_RS05020"
                     /old_locus_tag="pgaptmp_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HGU4194569.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8453..9445
                     /gene="proX"
                     /locus_tag="ACHKNB_RS05025"
                     /old_locus_tag="pgaptmp_001005"
     CDS             8453..9445
                     /gene="proX"
                     /locus_tag="ACHKNB_RS05025"
                     /old_locus_tag="pgaptmp_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HGU4194570.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9736..10920
                     /locus_tag="ACHKNB_RS05030"
                     /old_locus_tag="pgaptmp_001006"
     CDS             9736..10920
                     /locus_tag="ACHKNB_RS05030"
                     /old_locus_tag="pgaptmp_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194571.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11044..11781
                     /gene="ygaZ"
                     /locus_tag="ACHKNB_RS05035"
                     /old_locus_tag="pgaptmp_001007"
     CDS             11044..11781
                     /gene="ygaZ"
                     /locus_tag="ACHKNB_RS05035"
                     /old_locus_tag="pgaptmp_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HGU4194572.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11771..12106
                     /gene="ygaH"
                     /locus_tag="ACHKNB_RS05040"
                     /old_locus_tag="pgaptmp_001008"
     CDS             11771..12106
                     /gene="ygaH"
                     /locus_tag="ACHKNB_RS05040"
                     /old_locus_tag="pgaptmp_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HGU4194573.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12197..12727
                     /gene="emrR"
                     /locus_tag="ACHKNB_RS05045"
                     /old_locus_tag="pgaptmp_001009"
     CDS             12197..12727
                     /gene="emrR"
                     /locus_tag="ACHKNB_RS05045"
                     /old_locus_tag="pgaptmp_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HGU4194574.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQIEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12854..14026
                     /gene="emrA"
                     /locus_tag="ACHKNB_RS05050"
                     /old_locus_tag="pgaptmp_001010"
     CDS             12854..14026
                     /gene="emrA"
                     /locus_tag="ACHKNB_RS05050"
                     /old_locus_tag="pgaptmp_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HGU4194575.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14043..15581
                     /gene="emrB"
                     /locus_tag="ACHKNB_RS05055"
                     /old_locus_tag="pgaptmp_001011"
     CDS             14043..15581
                     /gene="emrB"
                     /locus_tag="ACHKNB_RS05055"
                     /old_locus_tag="pgaptmp_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HGU4194576.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15645..16160)
                     /gene="luxS"
                     /locus_tag="ACHKNB_RS05060"
                     /old_locus_tag="pgaptmp_001012"
     CDS             complement(15645..16160)
                     /gene="luxS"
                     /locus_tag="ACHKNB_RS05060"
                     /old_locus_tag="pgaptmp_001012"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGU4194577.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(16310..17866)
                     /gene="gshA"
                     /locus_tag="ACHKNB_RS05065"
                     /old_locus_tag="pgaptmp_001013"
     CDS             complement(16310..17866)
                     /gene="gshA"
                     /locus_tag="ACHKNB_RS05065"
                     /old_locus_tag="pgaptmp_001013"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HGU4194578.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17939..18367)
                     /gene="yqaA"
                     /locus_tag="ACHKNB_RS05070"
                     /old_locus_tag="pgaptmp_001014"
     CDS             complement(17939..18367)
                     /gene="yqaA"
                     /locus_tag="ACHKNB_RS05070"
                     /old_locus_tag="pgaptmp_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HGU4194579.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18364..18930)
                     /gene="yqaB"
                     /locus_tag="ACHKNB_RS05075"
                     /old_locus_tag="pgaptmp_001015"
     CDS             complement(18364..18930)
                     /gene="yqaB"
                     /locus_tag="ACHKNB_RS05075"
                     /old_locus_tag="pgaptmp_001015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HGU4194580.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19211..19287)
                     /locus_tag="ACHKNB_RS05080"
                     /old_locus_tag="pgaptmp_001016"
     tRNA            complement(19211..19287)
                     /locus_tag="ACHKNB_RS05080"
                     /old_locus_tag="pgaptmp_001016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19251..19253),aa:Arg,seq:acg)
     gene            complement(19486..19562)
                     /locus_tag="ACHKNB_RS05085"
                     /old_locus_tag="pgaptmp_001017"
     tRNA            complement(19486..19562)
                     /locus_tag="ACHKNB_RS05085"
                     /old_locus_tag="pgaptmp_001017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19526..19528),aa:Arg,seq:acg)
     gene            complement(19626..19702)
                     /locus_tag="ACHKNB_RS05090"
                     /old_locus_tag="pgaptmp_001018"
     tRNA            complement(19626..19702)
                     /locus_tag="ACHKNB_RS05090"
                     /old_locus_tag="pgaptmp_001018"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19666..19668),aa:Arg,seq:acg)
     gene            complement(19900..19976)
                     /locus_tag="ACHKNB_RS05095"
                     /old_locus_tag="pgaptmp_001019"
     tRNA            complement(19900..19976)
                     /locus_tag="ACHKNB_RS05095"
                     /old_locus_tag="pgaptmp_001019"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19940..19942),aa:Arg,seq:acg)
     gene            complement(19980..20072)
                     /locus_tag="ACHKNB_RS05100"
                     /old_locus_tag="pgaptmp_001020"
     tRNA            complement(19980..20072)
                     /locus_tag="ACHKNB_RS05100"
                     /old_locus_tag="pgaptmp_001020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20036..20038),aa:Ser,seq:gct)
     gene            complement(20388..20573)
                     /gene="csrA"
                     /locus_tag="ACHKNB_RS05105"
                     /old_locus_tag="pgaptmp_001021"
     CDS             complement(20388..20573)
                     /gene="csrA"
                     /locus_tag="ACHKNB_RS05105"
                     /old_locus_tag="pgaptmp_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HGU4194581.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20808..23438)
                     /gene="alaS"
                     /locus_tag="ACHKNB_RS05110"
                     /old_locus_tag="pgaptmp_001022"
     CDS             complement(20808..23438)
                     /gene="alaS"
                     /locus_tag="ACHKNB_RS05110"
                     /old_locus_tag="pgaptmp_001022"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGU4194582.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23566..24066)
                     /gene="recX"
                     /locus_tag="ACHKNB_RS05115"
                     /old_locus_tag="pgaptmp_001023"
     CDS             complement(23566..24066)
                     /gene="recX"
                     /locus_tag="ACHKNB_RS05115"
                     /old_locus_tag="pgaptmp_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGU4194583.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(24309..25370)
                     /gene="recA"
                     /locus_tag="ACHKNB_RS05120"
                     /old_locus_tag="pgaptmp_001024"
     CDS             complement(24309..25370)
                     /gene="recA"
                     /locus_tag="ACHKNB_RS05120"
                     /old_locus_tag="pgaptmp_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGU4194584.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNSETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25450..25947)
                     /gene="pncC"
                     /locus_tag="ACHKNB_RS05125"
                     /old_locus_tag="pgaptmp_001025"
     CDS             complement(25450..25947)
                     /gene="pncC"
                     /locus_tag="ACHKNB_RS05125"
                     /old_locus_tag="pgaptmp_001025"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HGU4194585.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26092..27177)
                     /gene="mltB"
                     /locus_tag="ACHKNB_RS05130"
                     /old_locus_tag="pgaptmp_001026"
     CDS             complement(26092..27177)
                     /gene="mltB"
                     /locus_tag="ACHKNB_RS05130"
                     /old_locus_tag="pgaptmp_001026"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HGU4194586.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27433..27996
                     /gene="srlA"
                     /locus_tag="ACHKNB_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /gene_synonym="gutA"
     CDS             27433..27996
                     /gene="srlA"
                     /locus_tag="ACHKNB_RS05135"
                     /old_locus_tag="pgaptmp_001027"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HGU4194587.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27993..28952
                     /gene="srlE"
                     /locus_tag="ACHKNB_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /gene_synonym="gutE"
     CDS             27993..28952
                     /gene="srlE"
                     /locus_tag="ACHKNB_RS05140"
                     /old_locus_tag="pgaptmp_001028"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HGU4194588.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28963..29334
                     /gene="srlB"
                     /locus_tag="ACHKNB_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /gene_synonym="gutB"
     CDS             28963..29334
                     /gene="srlB"
                     /locus_tag="ACHKNB_RS05145"
                     /old_locus_tag="pgaptmp_001029"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HGU4194589.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            29338..30117
                     /gene="srlD"
                     /locus_tag="ACHKNB_RS05150"
                     /old_locus_tag="pgaptmp_001030"
     CDS             29338..30117
                     /gene="srlD"
                     /locus_tag="ACHKNB_RS05150"
                     /old_locus_tag="pgaptmp_001030"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HGU4194590.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            30222..30581
                     /gene="gutM"
                     /locus_tag="ACHKNB_RS05155"
                     /old_locus_tag="pgaptmp_001031"
     CDS             30222..30581
                     /gene="gutM"
                     /locus_tag="ACHKNB_RS05155"
                     /old_locus_tag="pgaptmp_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HGU4194591.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30648..31421
                     /gene="srlR"
                     /locus_tag="ACHKNB_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /gene_synonym="gutR"
     CDS             30648..31421
                     /gene="srlR"
                     /locus_tag="ACHKNB_RS05160"
                     /old_locus_tag="pgaptmp_001032"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HGU4194592.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31414..32379
                     /gene="gutQ"
                     /locus_tag="ACHKNB_RS05165"
                     /old_locus_tag="pgaptmp_001033"
     CDS             31414..32379
                     /gene="gutQ"
                     /locus_tag="ACHKNB_RS05165"
                     /old_locus_tag="pgaptmp_001033"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HGU4194593.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(32376..33890)
                     /gene="norR"
                     /locus_tag="ACHKNB_RS05170"
                     /old_locus_tag="pgaptmp_001034"
     CDS             complement(32376..33890)
                     /gene="norR"
                     /locus_tag="ACHKNB_RS05170"
                     /old_locus_tag="pgaptmp_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HGU4194594.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            34077..35516
                     /gene="norV"
                     /locus_tag="ACHKNB_RS05175"
                     /old_locus_tag="pgaptmp_001035"
     CDS             34077..35516
                     /gene="norV"
                     /locus_tag="ACHKNB_RS05175"
                     /old_locus_tag="pgaptmp_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HGU4194595.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            35513..36646
                     /gene="norW"
                     /locus_tag="ACHKNB_RS05180"
                     /old_locus_tag="pgaptmp_001036"
     CDS             35513..36646
                     /gene="norW"
                     /locus_tag="ACHKNB_RS05180"
                     /old_locus_tag="pgaptmp_001036"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HGU4194596.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36774..39026)
                     /gene="hypF"
                     /locus_tag="ACHKNB_RS05185"
                     /old_locus_tag="pgaptmp_001037"
     CDS             complement(36774..39026)
                     /gene="hypF"
                     /locus_tag="ACHKNB_RS05185"
                     /old_locus_tag="pgaptmp_001037"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HGU4194597.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(39179..39706)
                     /gene="hydN"
                     /locus_tag="ACHKNB_RS05190"
                     /old_locus_tag="pgaptmp_001038"
     CDS             complement(39179..39706)
                     /gene="hydN"
                     /locus_tag="ACHKNB_RS05190"
                     /old_locus_tag="pgaptmp_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HGU4194598.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39855..40865)
                     /gene="ascG"
                     /locus_tag="ACHKNB_RS05195"
                     /old_locus_tag="pgaptmp_001039"
     CDS             complement(39855..40865)
                     /gene="ascG"
                     /locus_tag="ACHKNB_RS05195"
                     /old_locus_tag="pgaptmp_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HGU4194599.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            41125..42582
                     /gene="ascF"
                     /locus_tag="ACHKNB_RS05200"
                     /old_locus_tag="pgaptmp_001040"
     CDS             41125..42582
                     /gene="ascF"
                     /locus_tag="ACHKNB_RS05200"
                     /old_locus_tag="pgaptmp_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HGU4194600.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            42591..44015
                     /gene="ascB"
                     /locus_tag="ACHKNB_RS05205"
                     /old_locus_tag="pgaptmp_001041"
     CDS             42591..44015
                     /gene="ascB"
                     /locus_tag="ACHKNB_RS05205"
                     /old_locus_tag="pgaptmp_001041"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HGU4194601.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
BASE COUNT        10409 a        11800 c        11217 g        10644 t
ORIGIN      
        1 agattattgg tgaacagagt ggtgctctct gctcgctaat tccgcaagcc tgacaaaacc
       61 gaagtgaata aaaatgcaat ataacagaat aaaagaaaac acccctcact ggtgctttct
      121 gctgttaatg tacattttct ggcataaaaa atgctgtaaa tgacccggtc acgacataca
      181 ccgaattctc tacgcttata gcgttacctc acccccaaac tttcattagt gaaattaaag
      241 gcaaggtcat atctaattga tttaattgaa aataaatcat atcggcatgt aaaaatccac
      301 agtgtacaaa aaacgagccg ttacggaata ttttatctac aaaaactgac taaataaaaa
      361 tttttcacta attgaatagt catagccagc gatatacgct atgcgaaaat gcagatggca
      421 atgagatcca ctgctttcat ctccattaac atcccattac gcttttatta aggagcatta
      481 gcatgttctc accgcagtca cgcttgcgtc atgcagttgc agatacgttc gcgatggttg
      541 tttactgttc tgtcgtgaac atgtgtattg aagttttcct ctccggaatg agcttcgaac
      601 agtcttttta ttccagattg gtagcgattc cggtgaacat cttaattgca tggccatacg
      661 gtatgtaccg cgatctgttt atgcgcgcgg cacgcaaagt tagcccgtcg ggctggataa
      721 aaaatctggc tgatatcctg gcttatgtga cgttccagtc accggtgtat gtggcgatct
      781 tgttagtggt gggcgcagac tggcatcaga ttatggcggc ggtcagttca aacatcgttg
      841 tttcgatgtt gatgggggcg gtttatggct acttcctcga ttattgccgc cgactgttta
      901 aagtcagccg ttaccagcag gtaaaagcct gactcttctt ttcgcgactg gcatcgccag
      961 tcgcgtcttt aactggcttc gccaaataaa ccattcaaat aacgttcaag cgcaacacgc
     1021 gaactaaagc catgcgggat accgtaatgc tcgtgtcgct cgcctgctaa atagagcgga
     1081 aattgctggt aatgatcgca agttttaata tcggaagcgg gttcagccag tgttgaactg
     1141 cgttctttta atgttggatg aattaccaga gcggtacgtc ccattcgcgc ttcgcgatta
     1201 acatagacat aattttcccc acggcggtaa ccatacgctt tctgggttac cacatcgaca
     1261 gtaaaaccga ctttttcaag tacgcgcgcc acctcgtctg gtcgtaaata catatttgat
     1321 cctcgttatt atcaaccgcg ggcctacctt acctgattgc gcattcacaa cgctttcaga
     1381 aaagtccata aagccgcgat gaacctcagt taagaaaatt atggtctaca ctgaaaatta
     1441 catcgaattc taatggggga tcatatgttt aaccgaccga accgcaacga cgttgatgat
     1501 ggcgtgcagg atatccagaa tgatgtcaat caattagctg acagcctcga atctgtattg
     1561 aagtcctggg gcagcgacgc caaaggggaa gctgaagccg cacgcagtaa agctcaggca
     1621 ttgctgaaag aaaccagggc gcgaatgcat ggtcgtactc gcgtccagca agccgcacgc
     1681 gatgccgttg gctgcgcgga ttcttttgtt cgtgaaagac cctggtgtag cgtgggtaca
     1741 gcagctgcgg taggtatttt tatcggcgca ctgttaagca tgcgcaaatc gtagtgcaaa
     1801 aatgataata aatacgcgtc tttgaccccg aagcctggct tcggggtttc tttttgcctg
     1861 gtgaatcaca aaaatccccc taccccgtca cgctcatatc cagggtaatt tcgaccacta
     1921 tttgctatat attgtgtggt tgaatctttt ttcaactaca tctagtatct ctgtatcaac
     1981 agagagacaa cccgacgcgt atcatcgcgc cgtatcttca ttttaaacgg aaatacgaat
     2041 catgcgcatt actatttaca ctcgtaacga ttgcgttcag tgccacgcca ccaaacgggc
     2101 gatggaaaac cggggctttg attttgaaat gattaatgtc gatcgcgttc ctgaagcagc
     2161 ggaagcgttg cgtgctcagg gctttcgtca gttgccggta gtgattgctg gcgatcttag
     2221 ctggtctggt ttccgtccgg acatgattaa ccgtctgcat ccagcgccac acgcggccag
     2281 tgcatgagcc agctcgtcta cttctccagc agctccgaaa acacgcagcg ttttatcgaa
     2341 cgtttaggtc tgcccgcggt gcgcatcccg ctcaatgagc gggaacggat tcaggtagac
     2401 gagccttaca tcctgatcgt gccctcttac ggcggcggcg gtacggctgg cgcggtgcca
     2461 cgacaggtaa ttcgcttttt aaacgacgag cacaaccggg cgttgcttcg cggcgttatt
     2521 gcttctggta atcgcaactt tggtgaggcg tatggccgcg ccggagatgt gattgcccgg
     2581 aaatgcggcg tgccgtggct gtaccgtttt gaactcatgg gtacgcaaag cgatatcgaa
     2641 aacgttcgta aaggagtaac cgaattttgg caacgacaac cgcagaatgc ctgacgcagg
     2701 aaacgatgga ttaccacgcg ctgaatgcga tgcttaacct ctacgatagc gcaggtcgca
     2761 ttcagttcga taaagaccgc caggccgttg acgcctttat tgcgacgcat gtgcgtccga
     2821 acagtgtgac cttcagtagc cagcagcagc gcctgaactg gctggtcaac gaaggttact
     2881 atgatgaaag cgttcttaat cgctactctc gcgactttgt cattacgctg tttgcccacg
     2941 cacacaccag cggttttcgt ttccagacat tcctcggggc atggaagttt tacaccagct
     3001 atacgttgaa gacattcgac ggtaaacgtt atctggaaga ttttgccgat cgagtcacga
     3061 tggtggcgct gacgctggca caaggcgatg cgacgctggc gttgcaactg accgatgaaa
     3121 tgctgtcagg acgctttcag ccagccacgc caacattcct caactgcggt aagcagcagc
     3181 gcggcgaact ggtttcctgt tttctgctgc gtattgaaga caatatggag tcgattggcc
     3241 gggcggtaaa ttccgcactg caactgtcga aacgcggcgg cggcgtggca tttttgctgt
     3301 cgaatctgcg ggaagcgggc gcgccaatta aacgtattga aaatcaatct tctggcgtaa
     3361 ttccggtgat gaaaatgctg gaagacgcat tttcctatgc caaccaactc ggcgcgcgtc
     3421 agggggctgg tgcagtctat ttacatgctc atcatcccga tattctgcgt tttctcgaca
     3481 cgaaacggga aaatgccgac gaaaaaatcc gcattaaaac actgtcgctt ggcgtggtga
     3541 tcccggatat cactttccat ctggcaaaag agaatgcgca gatggcgctg ttttcgccct
     3601 atgacgttga gcgagtttat ggcaagccgt ttgccgatat cgccattagc gaacactatg
     3661 acgaactggt tgccgatgaa cgcatccgca aaaaatacct caacgcccgt gatttcttcc
     3721 agcgactggc agaaatccag tttgagtccg gctatcccta catcatgtat gaagacacgg
     3781 taaaccgtgc taaccctatc gccgggcgca taaatatgag taatctctgc tcagaaattt
     3841 tgcaggttaa cagcgcctca gagtatgacg agaatctcga ctatacctac acaggccatg
     3901 atatctcctg caatttaggt tcgttgaata ttgcgcacac catggattcc cccgattttg
     3961 cccgcacggt agagactgcc gtgcgcggtt taacggcagt atcagatatg agtcatatcc
     4021 gcagcgtgcc gtccatcgaa gccggaaatg ccgcctcgca cgccatcgga ctggggcaga
     4081 tgaatttaca cggctatctg gcgcgagaag gcatcgctta tggttcgccg gaagcactgg
     4141 atttcaccaa tctctatttc tatgccatca cctggcacgc actgcgtacc tcgatgttgc
     4201 tggcacgcga acgcggtgaa accttcgccg ggttcaaaca gtcacgctat gccagtggtg
     4261 aatattttag ccaatatctg caagggaact ggcagccgaa aacggcgaaa gttggcgaac
     4321 tgtttacccg tagcggtatt acgttaccta cccgtgagat gtgggcgcag ctgcgcgacg
     4381 acgtgatgcg ctacggcatt tacaaccaga atcttcaggc ggtgccgcca accggttcta
     4441 tctcttatat caaccatgct acgtcgagta ttcatccgat tgtggcgaaa gtagagatac
     4501 gcaaagaggg caaaacagga cgcgtttact accctgcccc gtttatgact aacgaaaatc
     4561 tggcgctata tcaggacgct tacgaaatcg gcgcagaaaa gatcatcgac acctacgcgg
     4621 aagcgactcg ccatgtcgat caggggctgt cgctgacgct gtttttcccc gataccgcca
     4681 ccactcgcga tatcaacaaa gcgcagattt acgcctggcg caaaggtatc aaaacgctct
     4741 attacatccg cctgcgtcag atggcgctgg aaggcactga aattgaaggc tgcgtctcct
     4801 gtgcacttta aggaatatct atgaaactct cacgtatcag cgccatcaac tggaacaaga
     4861 tatctgacga taaagatctg gaggtgtgga atcgcctgac cagcaatttc tggctaccag
     4921 aaaaggtgcc gctgtcgaac gatattcctg cctggcagac attaactgtc gtagaacaac
     4981 aactgacgat gcgcgttttt actggcctga cgctgctcga cacgctgcaa aatgttatcg
     5041 gcgcgccttc gctgatgccc gatgcactca cgcctcatga agaagcggta ttatcgaata
     5101 tcagctttat ggaagcggtt catgcccgct cttacagttc gattttctcg acgctgtgtc
     5161 agaccaaaga tgtcgatgcc gcctacgcct ggagtgaaga aaacgcaccg ttgcagcgaa
     5221 aagctcagat tattcagcaa cattatcgcg gtgatgatcc gctgaaaaag aaaatcgcca
     5281 gtgtgtttct tgaatctttt ttgttctatt ccggtttctg gctgccgatg tatttttcca
     5341 gccgcggaaa gctgaccaat accgcagatc tgatccgtct gattatccgc gatgaagcag
     5401 tccacggtta ctacataggc tataaatatc agaaaaacat ggaaaagata tctctgggac
     5461 aacgtgaaga gttgaagagt ttcgccttcg atttgttgct ggaactctac gacaacgagt
     5521 tgcaatacac cgatgagctg tacgccgaaa ccccgtgggc tgacgatgtg aaagcgtttc
     5581 tctgttacaa cgccaataag gctttgatga atctgggcta cgaaccgtta tttcccgcag
     5641 aaatggcgga agtgaatccg gcaatcctcg ccgcgctttc gccgaatgcc gatgaaaatc
     5701 acgatttctt ttccggttca ggctcctctt atgtgatggg gaaagcggtt gaaacagaag
     5761 atgaagactg gaatttctga gggtgttatt ttcaaaaata tcactacccg cagcagggaa
     5821 ataattcccg ccaaatagct ttttatcacg caaatattct gtagtgatct acactgatac
     5881 tctgttgcat tattcgcctg aaaccacaat attcaggcgt tttttcgcta tctttgacaa
     5941 aaaatatcaa cttttctcga tttgctctca gcccttatat cacgggaaat tccggcgatt
     6001 tactcgcatc aatattcatg ccacatttgc catcaggggt tgcctcagat tctcagtatg
     6061 ttagggtaga aaaaagtgac tatttccatt gggtaatata tcgacataaa caaataacag
     6121 gaatctttct attgcatggc aattaaatta gaaattaaaa atctttataa aatatttggc
     6181 gagcatccac agcgagcgtt caaatatatc gaacaaggac tttcaaaaga acaaattctg
     6241 gaaaaaactg ggctatcgct tggcgtaaaa gacgccagtc tggccattga agaaggcgag
     6301 atatttgtca tcatgggatt atccggctcg ggtaaatcca caatggtacg ccttctcaat
     6361 cgcctgattg aacccacccg cgggcaagtg ctgattgatg gtgtggatat tgccaaaata
     6421 tccgacgccg aactccgtga ggtgcgcaga aaaaagattg cgatggtctt ccagtccttt
     6481 gccttaatgc cgcatatgac cgtgctggac aatactgcgt tcggtatgga attggccgga
     6541 attaatgccg aagaacgccg ggaaaaagcc cttgatgcac tgcgtcaggt cgggctggaa
     6601 aattatgccc acagctaccc ggatgaactc tctggcggga tgcgtcaacg tgtgggatta
     6661 gcccgtgcat tagcgattaa tccggatatt ttattaatgg atgaagcctt ctcggcgctc
     6721 gatccattaa ttcgcaccga gatgcaggat gagctggtaa aattacaggc gaaacatcag
     6781 cgcaccattg tctttatttc ccacgatctt gatgaagcca tgcgtattgg caaccgaatt
     6841 gccattatgc aaaatggtga agtggtacag gtcggcacac cggatgaaat tctcaataat
     6901 ccggcgaatg attatgtccg taccttcttc cgtggcgtcg atattagtca ggtattcagt
     6961 gcgaaagata ttgctcgccg gacaccgaac ggcttaattc gtaaaacccc tggcttcggc
     7021 ccacgttcgg cgctgaaatt attgcaggat gaagaccgcg aatatggcta cgttatcgaa
     7081 cgcggtaata agtttgtcgg cgcagtttcc atcgattcgc ttaaaaccgc gttaacgcag
     7141 cagcaaggtc ttgatgcggc gctgattgat gcgccgttag cagtcgatgc acaaacgcct
     7201 cttagcgagt tgctctctca tgtcggacag gcaccctgtg cggtgcccgt ggtcgacgag
     7261 gaccaacagt atgtcggcat catttcgaaa ggaatgctgc tgcgcgcttt agatcgtgag
     7321 ggggtaaata atggctgatc aaaataatcc gtgggatacc acgccagcgg cggacagtgc
     7381 cgttcaatcc gcagacgcct ggggtacacc ggcgactgca ccgactgacg gtggtggtgc
     7441 tgactggctg accagtacgc ctgcgccaaa cgtcgagcat tttaatattc tcgatccgtt
     7501 ccataaaacg ctgatcccgc tcgacagttg ggtcactgaa gggatcgact gggttgttac
     7561 ccatttccgt cccgtcttcc agggcgtgcg cgttccggtt gattacattc tcaacggttt
     7621 ccagcaattg ctgctgggta tgcccgcacc ggtggcgatt atcgttttcg ctctcatcgc
     7681 ctggcagatt tccggggtcg gaatgggcgt ggcgacgctg gtttcgctga ttgccatcgg
     7741 cgcaatcggt gcctggtcgc aggccatggt taccctggcg ctggtgttaa ccgccctgct
     7801 gttctgtatc gtcattggtt tgccgctggg gatctggctg gcgagaagtc cgcgagcggc
     7861 gaaaattatt cgtccactgc ttgatgccat gcagaccacg ccagcgtttg tttatctggt
     7921 gccaatcgtc atgctatttg gtatcggtaa cgtgccgggc gtggtggtga cgatcatctt
     7981 tgctctgccg ccgattatcc gtctgaccat tctggggatt aaccaggttc cggcggatct
     8041 gattgaagcc tcgcgctcat tcggtgccag cccgcgccag atgctgttca aagttcagtt
     8101 accgctggcg atgccgacca ttatggcggg cgttaaccag acgctgatgc tggccctttc
     8161 tatggtggtc attgcctcga tgattgccgt cggcgggctg ggtcagatgg tacttcgcgg
     8221 tatcggtcgt ctggatatgg ggcttgccac cgttggcggc gtcgggattg tgatcctcgc
     8281 cattatcctc gatcgcctga cgcaggccgt tgggcgcgac tcacgcagtc gcggcaaccg
     8341 tcgctggtac accactggcc ctgttggtct gctgacccgc ccattcatta agtaactctg
     8401 cacttgcccg gtgacgccgg gcattatcac cctgccaaaa aaaggaataa caatgcgaca
     8461 tagcgtactt tttgcgacag cgtttgccac gcttatctct acacaaactt ttgctgccga
     8521 tctgccgggc aaaggtatta ctgtaaatcc agttcaaagc accatcaccg aagaaacctt
     8581 ccagacgctg ctggtcagcc gtgcgctgga gaaattaggc tataccgtca acaaacccag
     8641 cgaagtagat tacaacgttg gctacacctc gcttgcttcc ggcgatgcaa ccttcaccgc
     8701 cgtgaactgg acgccactgc atgacaacat gtacgaagct gccggtggcg ataagaaatt
     8761 ttatcgtgaa ggggtatttg ttaacggcgc ggcacagggt tacctgatcg ataagaaaac
     8821 cgccgaccag tataaaatca ccaacatcgc acaactgaaa gatccgaaga tcgccaaact
     8881 gttcgatacc aacggcgacg gtaaagcgga tttaaccggt tgtaaccctg gctggggctg
     8941 cgaaggtgcc atcaaccacc agcttgccgc gtatgaactg accaacaccg tgacgcataa
     9001 tcaggggaac tacgcagcga tgatggccga caccatcagt cgctacaaag agggcaaacc
     9061 ggtgttttat tacacctgga cgccgtactg ggtgagtaat gagctgaagc cagggaaaga
     9121 tgtggtctgg ttgcaggtgc cgttctccgc actgccgggc gataaaaatg ccgataccaa
     9181 actgccgaat ggcgcgaatt acggcttccc ggtcagcacc atgcatatcg ttgccaacaa
     9241 agcctggacc gagaaaaatc cggcggcagc caaactgttt gccattatgc agttgccagt
     9301 ggccgatatt aacgcccaga acgccattat gcatgacggc aaagcctcag aaggcgatat
     9361 tcagggccac gttgatggct ggatcaaagc ccaccagcag cagttcgatg gctgggtgaa
     9421 tgaggcgctg gcagcgcaga agtaattttt attcaggcgg ataaggcgtt tttgcctcat
     9481 ccgccgctct gtacacaatg cctgatgcga cgctgacgcg tcttatcatg cctacaaaat
     9541 acactcattc gtatggcgga taaggcgttt tcgccgcatc cgccgttctg tgcacaatgc
     9601 ctgatgcgac gctggcgcgt cttatcatgc ctacaaacca catcgcacat ttcaacaatc
     9661 tatctttcat cccatattca tcaacatccg ctattattga tttccagctt aatcatcacc
     9721 tgatgaacaa aaataatgac taaacctaat catgagctta gcccggcgct gatcgtgctg
     9781 atgtctatcg ccaccggtct ggcggtagcc agtaactatt acgcccagcc attgctcgac
     9841 accatcgcgc gtaacttttc cctttccgcc agttcggcag gctttattgt taccgccgcg
     9901 caattgggct atgcagcagg tctgctgttt cttgttcccc tcggtgatat gtttgaacgc
     9961 cgccgcctga ttgtctcgat gaccttactg gcagcgggcg gtatgttaat cactgccagc
    10021 agtcagtcac tggcgatgat gatcctcggt acggcattaa ccggtttatt ctctgtcgtg
    10081 gcacaaattc tggttccgct ggcagcgacg ctggcttcac cggacaaacg cggcaaagtg
    10141 gttggcacta ttatgagcgg tctgctgttg gggatcttac tcgcacggac cgttgccgga
    10201 ttgctggcga atctcggcgg ctggcgcacc gtcttttggg ttgcctcggt gttaatggca
    10261 ctgatggcgc tggcattatg gcgtggtctg ccacaaatga aatcagaaac ccacctcaac
    10321 tacccacagt tgttaggttc cgttttcagc atgtttatca gcgataagat cctgcgcacc
    10381 cgcgcattgc tgggctgcct gacctttgcc aacttcagca ttctctggac ctcaatggcc
    10441 tttttgcttg ccgctccacc ttttaactac agcgatggtg tcattggtct gtttggactt
    10501 gcgggagctg ccggagcgtt gggcgctcgt ccggcgggcg gttttgccga taagggtaaa
    10561 tcgcaccaca ccacaacttt cggtctgctg ctgctattac tttcatggct ggcgatctgg
    10621 tttggtcaca cttccgtact ggcgctgatt atcggcatcc tggtgctgga tctcaccgta
    10681 cagggcgtgc atatcactaa ccagacggta atttatcgaa tacaccctga tgcgcgtaat
    10741 cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg ttcgctaatt
    10801 tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg cgcgacgatt
    10861 gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc cgcaaattaa
    10921 gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag cccatcagtc
    10981 tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt aagcgtacac
    11041 tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat ggaaggatgc
    11101 aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt cggtctgaat
    11161 gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat catttatgca
    11221 ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt gtgggttgct
    11281 gcactgaccg tcatggcaat ggatgttcgc catgtgttgt atggcccgtc actgcgtagc
    11341 cgtattattc agcgtctgca aaaatcgaaa accgccctgt gggcatttgg cctgacggat
    11401 gaggtttttg ccgccgcaac cgcaaaactg gtacgcaata atcgccgctg gagcgagaac
    11461 tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac ggtaataggg
    11521 gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc attaggtttt
    11581 atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa acaatctctt
    11641 tgcgttaccg cagcgttagt tggtgccctt gcaggcgtaa cgctattttc tattcccgtc
    11701 gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc attctggcaa
    11761 ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg tggcgaatta
    11821 ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa ccaaacgtgg
    11881 cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc tgctggttgt
    11941 ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc tggtcggctt
    12001 cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa cactgcttag
    12061 tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca caagaatcat
    12121 ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg gctgaaatta
    12181 atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc taaaatttcg
    12241 cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc tttgcatgca
    12301 catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg ggattaacga
    12361 gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta ttcagccttc
    12421 tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg ccgatgaact
    12481 ggaaaaacgc ggttggatcg aacgtcgtga aagcgataac gatcgccgct gcctgcatct
    12541 gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc agcataactg
    12601 cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagatcg agcaaatcac
    12661 ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc tcgaagcgat
    12721 gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat gaccggccag
    12781 catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag ttaagaagat
    12841 cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac cggtaaagaa
    12901 gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa ttattgccgt
    12961 agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg atgacgcata
    13021 cgtggcaggg aatcaaattc aaattatgtc tcaggtgtct ggcagcgtga cgaaagtctg
    13081 ggccgataac accgattttg taaaagaagg cgacgtgctg gtcactctcg acccgacaga
    13141 tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc gccaaaccca
    13201 ccagctgatg attaacagca agcagttgca ggcgaatatt gaggtgcaga aaatcgccct
    13261 cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca acctgattgg
    13321 tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc aactggacgt
    13381 cgcgattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac tggaagatca
    13441 gccagccgtg caacaggctg ccaccgaagt acgtaacgcc tggctggcgc tggagcgtac
    13501 tcgtattgtc agtccgatga ccggttatgt ctcccgccgc gcggtacagc ctggggcgca
    13561 aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt gggtggatgc
    13621 caactttaaa gagacgcaga ttgccaatat gcgtatcggt cagccggtca ctatcaccac
    13681 ggatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg atatgggcac
    13741 aggtagcgcg ttctcactgc ttccagcgca aaatgcgacc ggtaactgga tcaaagtcgt
    13801 tcagcgtctg cctgtgcgta tcgaactgga ccagaaacag ctggagcaat atccgctgcg
    13861 tatcggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc aggtactggc
    13921 aaataaagta cgttccactc cggtagcggt aagcaccgcg cgtgaaatca gcctggcacc
    13981 tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca gaggtgcgtg
    14041 tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg attgcgctgt
    14101 cactggcgac attcatgcag gtgctggact ccaccattgc taacgtggcg atccccacta
    14161 tcgccgggaa tctgggctca tcgctcagcc agggaacgtg ggtaatcact tctttcgggg
    14221 tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc ggggaagtga
    14281 aactgttcct ttggtccacc atcgcctttg ctattgcgtc gtgggcgtgt ggtgtctcca
    14341 gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc gggccgttga
    14401 tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc tcgatcgcgc
    14461 tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc ctcggcggtt
    14521 atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg attggcgtgg
    14581 cggtggtgtt gatgacactg caaactctgc gcggacgtga aacccgcacc gaacggcggc
    14641 ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg cagattatgc
    14701 tcgaccgcgg taaagagctg gactggtttt catcacagga aattatcatc cttaccgtgg
    14761 tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat aacccgatag
    14821 tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt atcagcctcg
    14881 cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag gaggtctacg
    14941 gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt ccggtgatcc
    15001 tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg gtaaccttca
    15061 gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa ccaggtatgg
    15121 attttggcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggcc tgcttcttta
    15181 tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg gcggcatcga
    15241 gcctctccaa ctttacgcgt acgctggcgg ggtctatcgg cacgtccatc accaccacca
    15301 tgtggaccaa ccgcgagtcg atgcaccacg cacagttaac cgagtcggta aacccgttta
    15361 acccaaacgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg caacagcagg
    15421 cctccggctg gattgcccag cagatcacca accaggggct gattatttcc gccaatgaga
    15481 tcttctggat gtcagccggg atattcctcg tcctgctggg gctggtgtgg tttgctaaac
    15541 cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta agccagttca
    15601 tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactaaat gtgcagttcc
    15661 tgcaacttct cttccggcag cgccagttct tcgttgctgt tgatgcgcac gtcacgttcc
    15721 agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta agtgccacac
    15781 tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac gtcttccatt
    15841 gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag actcatataa
    15901 aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt accgttaaga
    15961 tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct ttctggcatc
    16021 acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc gccatgcggg
    16081 gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg atcgactgtg
    16141 aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat tttctgaact
    16201 ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca tcatccctga
    16261 attcagagat gaaattttgg ccactcacga gtggcctttt tcttttctgt caggcgtgtt
    16321 tttccagcca caccgcaaac ggttcggtat ctgcggcttc catttcctgc tgacggcgtt
    16381 cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc ggctcttcac
    16441 gcagcagatt acggtacgct tcagcaaacg ctttgcctgt tccgccaata ccagtatcaa
    16501 tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag caggcaacca
    16561 gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc gtttgcgcga
    16621 cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac tgcgcggttt
    16681 cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc cggttccagt
    16741 taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc agcgcacacc
    16801 agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc ggcgagaacg
    16861 ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac agcgcatcag
    16921 aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac agttcgtttt
    16981 caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca ataccaatct
    17041 tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg tactcgtaaa
    17101 gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg agatcgctca
    17161 aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc tcaaacggca
    17221 gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca ccaaacagat
    17281 aaggaatgac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag cccgcagaaa
    17341 ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag aatgccattg
    17401 gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg tagcgatttt
    17461 tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg tactgtgcca
    17521 gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac atccgctcat
    17581 cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcaac atatgttcaa
    17641 tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg gtagtaatcc
    17701 atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc agtgtgccat
    17761 cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt aacgcctgag
    17821 gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg acctcccgcc
    17881 tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag gcctacaatt
    17941 agtgccacca catcatgccc tgaacggtag ctgctgcaac tgcaacatag cgtaacgctt
    18001 taccaaggca caaaaaaaag attaccggtc cccacgagat gcgcatccat cccgctaaca
    18061 gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact gcaccatagc
    18121 gtttcagcca gccagtagct ttctcttgcc agcgcgatgt tttacgcaat ggaaagaaac
    18181 gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct gttaaaacta
    18241 aaacccaggg ataactgatc ccggaaagca acattgccac cagcacgact tcggagttgc
    18301 cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc gataacgctt
    18361 cactcacagc aagcgaacat ccacggcgtc catgcctgct gcacgggccg cctgaatacc
    18421 gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac gctgcgcgca
    18481 caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag cgacgacggc
    18541 gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg cgctttcact
    18601 ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt caacaagagg
    18661 aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt cacgcgctaa
    18721 cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa tacgccaggt
    18781 gggcgatcca ttaagcgcaa tcattgcctg aacatcgtac tgaagaccgt agtgccctaa
    18841 tacttcgcgc cacgctttac ggtgcgtagg ctccgtatcc aggattgtgc catccatatc
    18901 aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa aatatgacgc
    18961 tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa attcaggaaa
    19021 tggataaagt agttatttag aaatgagata tttttgaagg aaatttttgg gagaagatgg
    19081 tgcatccggg aggattactc actggcgttc gcccttcggg ccgttgctga cgcaacgtta
    19141 tcctccctgg tgcctgcggt taataccgct atctcttcga taccttcatt gctgaaggtt
    19201 acggagaata tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag
    19261 tactctatcc agctgagcta cggatgcatc gggaaaattt attttactgc tgatattgat
    19321 atcgctacta aagccatatc aagtaagaga tggtgcatcc gggaggatta ctcgccttgc
    19381 ggctcgccct tcgggccgtt gctaaagcaa cgttatcatc cctggtgcct gcggttaatt
    19441 ccgctacctc ttcgatacct tcattgctga agatttcgga gaatatggtg catccgggag
    19501 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
    19561 gcatcgggaa aatttatttt actgctgata ttgataccgc tactaaagcc atatcaagta
    19621 agagatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc
    19681 tatccagctg agctacggat gcatcgggaa acttacttta ctgcagattt tttgataccg
    19741 ctactaaagc cgtatcaagt aagagatggt gcatccggga ggattactcg ccttgcggct
    19801 cgcccttcgg gccgttgcta aagcaacgtt atcctccctg gtgcctgcgg ttataccgcc
    19861 acctcttcca taccttcatc gctgaaagta cgtagaatat ggtgcatccg ggaggattcg
    19921 aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcaaat
    19981 ggcggtgagg cggggattcg aaccccggat gcagcttttg accgcatact cccttagcag
    20041 gggagcgcct tcagcctctc ggccacctca ccacacgcct cttacgagtg cttcgaagaa
    20101 cttgtttatc gctcatcgtc gctgcgtggc gcacatatta ctttctgaga cttataagtc
    20161 aaacaatttt tcctacactt ttatcgtttg cacacttcac gttcaattag tctgtaaaaa
    20221 cgtcaaaaag agtgttttat caacagaaaa atggaggtct gacagatagt agtaatgcaa
    20281 aaaaatggag acttaagttg aatgaacggg agtaaagcga aaagactata gagtgaagga
    20341 gaaattttga gggtgcgtct caccgataaa gatgagacgc ggaaagatta gtaactggac
    20401 tgctgggatt tttcagcctg gatacgctgg tagatctctt cacggtgaac agaaacttcc
    20461 ttcggggcat ttacgccaat acgtacctgg ttgcccttta cccctaaaac tgtcacggtg
    20521 acctcatccc caatcatgag ggtctcacca actcgacgag tcagaatcag cattctttgc
    20581 tccttgaaag attaaaagag tcgggtctct ctgtatcccg gcattatcca tcatataacg
    20641 ccaaaaagta agcaatgaca aacacattac atgtaagcag tcatggcatt acattctgtt
    20701 aaacctaagt ttagccgata tacacaactt caacctgact ttatcgttgt cgatagcgtt
    20761 aatgcgaatg ccgtgaagcg agtccacggc attgcctgac gcttatatta ttgcaatttc
    20821 gcgctgaccc agcctttcac actggctaac gctgcaggta aggccgcagc atccgtacca
    20881 ccggcttgcg ccatgtcagg acgtccacca cccttgccgc ccacctgctg agcgaccata
    20941 ccaatcagtt cccctgcttt cacacgatct gtgacgtcct tagatacgcc tgcaatcaga
    21001 gaaaccttac cttcggctac cgttgccagc acgataattg tcgaccccag ctgatttttt
    21061 aaatcgtcaa ccatggtacg caacattttc ggctcaacac cgctaagctc gctaaccaac
    21121 agcttaacac cattaacatc aattgcctta ctggaaagat ttgcgctctc ctgtgcggca
    21181 gcttgttctt taagctgttg tagttctttt tctaactgac gcgtacgttc cagtactgag
    21241 cgcactttat ccgccagatt attgctatcg cctttcagca gatgcgcgac ttcgcttaag
    21301 cgatcgctgt ctgcatgaac ggtggtgata gcgccttctc cggttaccgc ttcgatacga
    21361 cgaacgcctg cagcagtacc cgattcagag atgatgcgga acagaccaat atcaccagtg
    21421 cggctggcgt gagtaccgcc acacagctcg gtggagaaat cgcccatgct cagcacgcgt
    21481 acacgctcat catacttctc gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg
    21541 agatccatga tgttggtttc gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct
    21601 tcgaccgcac gaatctcttc cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc
    21661 accttgtcgt taaccagtga acctttctgc gatacatgag tccccagaac ctggcgcagc
    21721 gcagcgtgca tcaggtgcgt tgcggagtga ttcagacgaa tacgggcgcg acgagcctca
    21781 tcaacatcag cctgcacagc gtcgcccact ttcagagaac ccgcagcaag tttaccgatg
    21841 tgaccaatcg cctggccgta tttctgcgta tcttccaccg cgaaggagaa gttagcgcct
    21901 ttcagttcgc ctttatcacc aacctgaccg ccggattccg catagaatgg tgtttgatcc
    21961 agcacgacca cagcttcctg gcctgcattg atggcgtcaa ccgctttacc atcaacaaac
    22021 agcgcggtca ctttgccgtt cagttccaga tggtcatagc ctttaaattc agatgcacta
    22081 tcaacacgga tcattgcgtt gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga
    22141 cgctgctctt ccattgctgc ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga
    22201 caaacatcag ccgtcaggtc aaccgggaag ccataggtgt cgtacagacg gaaagccgtt
    22261 tcaccatcca gcgtatcacc agaaagtttt gccagctctt catccagcaa cgccagaccg
    22321 cgctccagag tacgggcaaa ctgctcttct tcagtcttca gcacctgctc aacctgcgcc
    22381 tgctggcgtt tcaggtcttc acccgcagag cccataacgt cgatcagcgg accaaccagt
    22441 ttgtagaaga aggtttcttt cgcgccgagc atattgccgt gacgcactgc gcgacgaatg
    22501 atacgacgca gtacataacc acggttttca ttggacggca ttacgccatc cgcgatcagg
    22561 aacgcacaag aacgaatgtg gtcagcgatt acgcgcagcg atttattgct cagatcggtc
    22621 gcgccagtga ctttcgctac cgcctggatc aacgtgcgga acaggtcgat gtcatagtta
    22681 gagttaacgt gttgcagcac cgcagcaata cgctccagac ccataccggt atctacagac
    22741 ggcttcggca gcggttccat cgtgccatct gcctggcggt tgaactgcat gaagacgatg
    22801 ttccagatct caatgtagcg gtcgccgtct tcttccggac ttcccggagg gccaccccaa
    22861 atgtggtcgc cgtgatcgta gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc
    22921 atctgccaga agttgtcaga tgcgtatggc gcacctttgt tatcgccgat gcgaataata
    22981 cgttcgcgcg ggatcccgac ttctttttcc cagatttcgt aggcttcgtc gtcgctttca
    23041 tagacggtaa cccacagacg ctctttcggc agggcaaacc atttttcgct ggtcagcagt
    23101 tcccatgcaa actgaatggc atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc
    23161 atttcgaaga aggtatggtg acgcgcggtg taaccgacgt tttccaggtc gttgtgttta
    23221 ccacccgcac gcacgcagcg ttgggaagtg gtagcgcggg aataattacg cttgtcgagc
    23281 ccaaggaaca catccttgaa ctggttcatc ccggcgttgg taaacaacaa agttgggtcg
    23341 ttatggggta ccagggagct gctggcaact acctgatgtc ccttactatg gaaaaagtcg
    23401 agaaacgcct gacggatctc agcggtgctc ttgctcataa ttatcctgaa atcaagctaa
    23461 cgaaatatcg ccaccagctc cagcgtgtct taaccgccgg gctggtaact gaaaagtggg
    23521 aataagataa gttttcttga ctgggaagta aaataccgta tgcgttcagt cggcaaaatt
    23581 tcgccaaata tcctggatat cttccatcag atagccacga tagagcagaa aacgctggat
    23641 cttaactttt tctgaaaaga cagttggcaa aggttcacca tattttcgcg tcgcctgatc
    23701 gcgcgccagt gcgcaccagt cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg
    23761 ggaaatacct ttctgattca gttcctgacg aatacgtgca ggtccataac ctttgcggct
    23821 acggctggcg ataaagcgcg caacaaatcg gctgtcatcg agatagccat gttcatggca
    23881 ccaggcaata acgcgctcgt aatcttctgc cgtagcatca atctcttctg ggccattttt
    23941 gcccataatc ggtgccgcga gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc
    24001 cagaatgcgt accgcacgat ccaacaggcg agcatatgcc gggcgacggg acgttgattc
    24061 tgtcatggca tatccttaca acttaaaaaa gcaaaagggc cgcagatgcg acccaggttt
    24121 tgctgataaa cagatttaat agtcaatgag tgtgcagaat gttcccgcag gcccaattcg
    24181 ttagaggatc gaggtgagag taatttttca cgccagtgct ggcctgttcc cctcacccta
    24241 accctctccc caaaggggcg aggggaccgt ccgtgcacac attatctatt atcatcaata
    24301 acgaacgatt aaaaatcttc gttagtttct gctacgcctt cgctgtcatc tacagagaaa
    24361 tccggcgttg agttcgggtt gctcagcagc aactcacgta ctttcttctc gatctctttc
    24421 gcggtttccg agttatcttt cagccaggca gttgcattcg ctttaccctg accgatcttc
    24481 tcacctttgt agctgtacca cgcgcctgct ttctcgatca gcttctcttt tacgcccagg
    24541 tcaaccagtt cgccgtagaa gttgatacct tcgccgtaga ggatctgaaa ttcagcctgt
    24601 ttaaacggtg cagcgatttt gttcttcacc actttcacgc gggtttcgct acccaccacg
    24661 ttttcgccct ctttcaccgc gccgatacga cggatgtcga gacgaacaga ggcgtagaat
    24721 ttcagcgcgt taccaccggt agtggtttcc gggttaccga acatcacacc aattttcata
    24781 cggatctggt tgatgaagat cagcagcgtg ttggactgct tcaggttacc cgccagctta
    24841 cgcatcgcct ggctcatcat acgtgccgca aggcccatgt gagagtcgcc gatttcgcct
    24901 tcgatttccg ctttcggcgt cagtgccgcc acggagtcaa cgacgataac gtctactgcg
    24961 ccagaacgcg ccagggcgtc acagatttcc agtgcctgct cgccggtgtc cggctgggag
    25021 cacagcaggt tatcgatatc gacgcccagt ttacgtgcgt agattgggtc cagcgcgtgt
    25081 tcagcatcga taaacgcaca ggttttacct tcacgctgcg ctgcggcgat cacctgcaac
    25141 gtcagcgtgg ttttaccgga agattccggt ccgtagattt cgacgatacg gcccatcggt
    25201 agaccacctg ccccaagcgc gatatccagt gaaagcgaac cggtagagat ggtttccaca
    25261 tccatggaac ggtcttcacc caggcgcatg atggagcctt taccaaattg tttctcaatc
    25321 tggcccagtg ctgccgccaa cgctttctgt ttgttttcgt cgatagccat ttttactcct
    25381 gtcatgccgg gtaataccgg atagtcaata tgttctgttg aagcaattat actgtatgct
    25441 catacagtat caagtgtttt gtagaaattg ttgccacaag gtctgcaatg catacgcagt
    25501 agcctgacga cgcaccgcat cacggtcgcc gctgaagcat tcccgccggg taatgccttc
    25561 accgcgggca gtggcaaaag caaaccagac ggtgccgaca ggcttctctt cactgccgcc
    25621 atccggcccg gcgataccac taatagacac ggcataatca gcacgagccg ctttcagtgc
    25681 gcctatcacc atttccacca cgacgggttc actcaccgcg ccatgctgcg ccagcgtctc
    25741 ttcgcgtacg ccgatcatct gcgctttggc ttcgttactg taggtgacaa atccgcgttc
    25801 aaaccaggcg gagctaccgg caatatcggt aatcactttc gctacccaac caccggtaca
    25861 agactcggca gttgttacgg ttgcgccacg ggctttcagc gcctgcccaa cctgttcact
    25921 taactgcatc agttcactgt cagtcatcac aacctctgtc agtcaaaaat ttatgccgga
    25981 caagatagca ctttcgtcag gaagatggtg ttggcgttgc aattttacga ggaggattca
    26041 gaaaaaagct gattagccag agggaagctc acgcccccct cttgcaaata gttactgcac
    26101 gcgcgccagc gccacggctt gtcctaactg ccagaccgcc attgcgtaat gggtgctgtg
    26161 gttataacgg gtaatggtgt agaagttcgg cagaccgtac cagtactggt aaccggtacc
    26221 gacatccaga cgcagcaggc tggcttgttg atggttgccc agcggctgct gtggcgttaa
    26281 acctgccgtg gcaagctgcg aaatgctgta cctggttttg aagccatttg gcaagcctgg
    26341 agcctgaccg tttgccatta ccgcgacctg atcgcctttc acccagccgt gcgctttgaa
    26401 atagttcgcc acgctaccga tcgcatcaac cggatcccac aggttgatat gcccgtcgcc
    26461 gctgaaatct accgcatatt gtttgtaaga cgacggcata aactgtccgt agcccatcgc
    26521 cccggcaaag gaacctttca gattgagcgg atcgtcctgc tcgtcgcgcg ccatcaacag
    26581 gaaggtttcc agctcgccag agaaatactc cgcgcggcgt gggtagttaa atgacagcgt
    26641 tgccagcgca tcgaggatgc gagttttccc catcacgcgc ccccagcggg tttcaacgcc
    26701 gataatcccg acgataattt ccggcggtac gccatacacc tgccacgcgc gattcaacgc
    26761 atcttcatac tgattccaga aaaccacacc gttctgcacg ttgtccggcg taataaattt
    26821 tttgcgataa cggagccatg cgccgttcgg acctgatggc ggtttcaccg atgtggttgg
    26881 tgcctggtta tccatcagcc gcagtaccga atccagacgt ttcgcctggg agagaatttc
    26941 ctgcaactgc tgacgatcga aaccgtgttt gttcaccatt ttgtcgatga actgctgggc
    27001 attcgggtta ttagcgaaat cgccgcccat ctgcatcaca ttgtgctgcg gctcaagcag
    27061 gaagccgcca gacggcgttc cggtggtcgt ttcagtctca gtaggttttg gcttgctgct
    27121 acaggcggca agcaacacaa aaaggggaag caatgttaca taacgacgct tgaacatgag
    27181 gggtccattt aacagattca accaggggca agtatggtaa agcatcacgc cccacacaag
    27241 gaagcggtag tcactgcccg atacggactt tacataactc aactcattcc cctcgctatc
    27301 cttttactca aactttcaaa ttaaaatatt tatctttcat tttgagatca aaataacact
    27361 tttaaatctt tcaatctgat tagattaggt tgccgtttgg taataaaaca ataaatcctg
    27421 aaggagagaa caatgataga aaccattact catggtgcag agtggtttat cgggctgttc
    27481 caaaagggcg gagaggtgtt taccgggatg gtgaccggca ttcttccgct gttgattagc
    27541 ctgctggtta tcatgaacgc actgattaat tttatcggtc agcatcgtat tgaacgtttt
    27601 gctcaacgtt gcgccggtaa ccctgtttcc cgttacctac tgttaccgtg cattggcacg
    27661 tttgtctttt gcaatccgat gaccttaagc ctgggtcgct ttatgccgga aaagtacaaa
    27721 cccagctact acgcggcggc ctcttatagc tgccactcaa tgaatggcct cttcccccat
    27781 atcaaccctg gcgaactgtt tgtttatctt ggcattgcca gcggtctgac aacgctgaac
    27841 ctgccacttg gcccactggc ggtgagttat ctgctggttg gtctggtcac caatttcttc
    27901 cgcggctggg tgaccgatct gaccaccgcc atttttgaga aaaagatggg cattcaactt
    27961 gaacaaaaag ttcacctagc aggagcaaca tcatgacgca tattcggatc gaaaaaggaa
    28021 cgggtggctg gggcggcccg cttgagctga aagccacacc gggaaaaaaa atcgtctata
    28081 tcaccgccgg tacccggcct gcgattgttg acaaactggc acagcttact ggctggcagg
    28141 ctattgacgg atttaaagaa ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg
    28201 actgtggcgg cacattacgc tgcggcatct atccgaaacg acgtattccc accattaata
    28261 tccactcgac gggcaagtcc ggtccgctgg cgcagtacat tgtggaagat atttatgtct
    28321 ctggcgtaaa agaagaaaac atcactgtag tgggtgatgc gacaccacaa ccctcttccg
    28381 tgggccgtga ctatgacacc agtaagaaaa tcaccgaaca aagcgatggt ttactggcga
    28441 aggtgggaat gggcatgggg tccgccgttg cggtgctgtt tcaatctggt cgtgacacca
    28501 tcgacactgt attaaaaacc attctgccgt ttatggcatt cgtctcggcg ctcattggca
    28561 tcattatggc ttctggcctt ggtgactgga ttgcccacgg tcttgctccg ctggcgagcc
    28621 atccactggg tctggtcatg ctggcgctca tctgctcctt cccactgctt tcacctttcc
    28681 tcggcccagg cgcagttatc gcacaggtta tcggcgtatt gattggcgtg cagattggtc
    28741 tcggcaatat tccgccgcat ctggctttac ctgcactgtt tgccatcaac gcgcaggcgg
    28801 cctgcgactt catcccggtc ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg
    28861 tcggtgtccc ttctgtactg gtgagccgct ttttaaccgg cgcgccgact gtactgatcg
    28921 cctggtttgt ctccggtttt atctatcaat agaggctgaa acatgaccgt tatttatcag
    28981 accaccatca cccgtatcgg cgcgagtgcc actgacgccc tcagcgacca gatgctcatc
    29041 acctttcgtg aaggcgcgcc agcggacctc gaagagtatt gcttcattca ttgccacggc
    29101 gagttgaaag gtgcactcca tcccggtttg caattttcac tcgggcagca tcgctatccg
    29161 gtgaccgctg ttggcagcgt ggcggaagac aaccttcgcg aactgggtca tgtcaccctg
    29221 cgcttcgatg gtttaaacga agcggaattt ccgggcactg tccatgtggc aggccctgta
    29281 cccgacgata tcgcgccggg atcggttttg aagtttgaat ctgttaagga gtaaaaaatg
    29341 aatcaggttg ccgttgtcat cggtggtggg caaaccttag gcgcgttcct gtgccacggt
    29401 ctggctgccg aggggtatcg cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat
    29461 gtggcacaag aaattaacgc cgaatatggt gaaggtatgg cgtacggttt tggtgctgac
    29521 gccaccagcg agcaaagcgt tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc
    29581 gtggatttgc tggtctatag cgccggaata gcaaaagcag cctttatcag cgacttccag
    29641 ctcggcgatt ttgaccgttc gctacaagtg aatctggtgg gttatttcct gtgtgcccgt
    29701 gaattttcgc gtttgatgat ccgcgacggg attcagggtc gcattattca gatcaactcg
    29761 aaatccggca aagtgggtag caaacacaac tctggctaca gcgcagcgaa atttggtggc
    29821 gtcgggctga ctcaatcact ggcgctggat ctggcggagt acggcattac ggtgcattca
    29881 ctgatgctcg gtaacctgct gaaatcgccg atgttccagt cactgttgcc acaatacgcg
    29941 accaagctgg gtatcaaacc ggatcaagtc gagcagtatt acatcgacaa agtaccgctc
    30001 aaacgcggct gcgattatca agatgtgctg aatatgctgc tgttctacgc cagtcctaag
    30061 gcgtcgtact gcaccggaca gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca
    30121 acagcggaga tccgttaagg atctccgtga gactatagaa tgcctgatgc gctacgctca
    30181 tcaggcatac aggacttccg ccactacatt aaggaaaagt tatggtatcc gcactcatca
    30241 ccgtcgccgt tatcgcctgg tgtgcgcaac tggccttagg cggctggcaa atttctcgtt
    30301 ttaaccgtgc cttcgacaca ctatgccagc aagggcgggt tggcgtgggc cgttccagcg
    30361 ggcgctttaa accgcgggtc gtggtcgcca tcgccctgga cgatcagcag cgcatcgtcg
    30421 acaccttgtt tatgaaagga ctgaccgtct tcgcccgacc gcaaaaaatt cccgcaatta
    30481 ccggtatgca tgcgggtgat ttacagcccg atgtgatctt tccccatgat ccactatcac
    30541 agaatgctct atcattggcg cttaaactga aacgtggata atttcgttgt gaatgttact
    30601 tgcttgcgaa gttatcattt tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc
    30661 agcgtcaggc cgccattctg gagtatctgc aaaagcaggg taaatgctcg gttgaagaat
    30721 tggcgcaata ctttgacacc acaggcacaa ccattcgcaa agatctggtc attctggaac
    30781 atgccggaac cgtcattcgt acttatggcg gagtggtgtt gaataaagag gaatccgatc
    30841 cgcctatcga tcataaaaca ctcatcaaca cccacaagaa agagctgatt gcagaagctg
    30901 ccgttagttt tatccatgat ggcgattcga tcattcttga tgctggcagt accgttttgc
    30961 agatggttcc cctgctctcg cgctttaata acatcacggt gatgaccaac agcctgcata
    31021 tcgtcaatgc gctatccgaa ctggataacg aacaaactat cctgatgcca ggcggaacgt
    31081 ttcgtaaaaa atcggcctca tttcacggac agctggcaga gaatgccttc gaacatttca
    31141 gtttcgataa attgtttatg ggcaccgacg gcatcgatct caatgcgggc gtaaccacct
    31201 tcaacgaggt ttataccgtc agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga
    31261 tggcggactc atcaaagttt ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg
    31321 tcgataagct gattaccgac gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga
    31381 aagggatcga tgtgatcata accggagaga gcaatgagtg aagcactaca gaacgcggga
    31441 cgtcagacgt taatgctgga gttgcaggaa gcaagccgtt taccggaacg tctgggcgat
    31501 gattttgttc gcgccgccaa tatcatcctg cactgcgaag gcaaagtggt ggtttcggga
    31561 attggcaaat cgggccacat tggtaagaaa atcgccgcaa cacttgccag taccggcaca
    31621 ccggcttttt ttgtccatcc ggcagaagca ctgcacggcg atctggggat gattgaaagc
    31681 cgcgatgtga tgctgtttat ctcttactcc ggtggcgcga aggaactgga tctgattatt
    31741 ccgcgtctgg aagataaatc tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg
    31801 ctgggcctgg cggcaaaagc ggtgctggat atctccgtag aacgcgaagc ctgcccgatg
    31861 caccttgcgc cgacctccag caccgtcaat accctgatga tgggtgacgc gctggcgatg
    31921 gcggtcatgc aggcgcgcgg atttaatgaa gaagattttg cccgctccca cccagccggg
    31981 gcactgggcg ctcgcttgct gaataaagtg catcatctga tgcgccgtga cgatgccatc
    32041 ccacaggtgg cgttaaccgc cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt
    32101 ctggggctgg tggcggtatg tgacgctcaa caacaggtac aaggcgtctt taccgacggc
    32161 gatttacgtc gctggctggt tggcggcggc gcactcacca cgccagttaa tgaagcgatg
    32221 acggtcggcg gcaccacgtt gcaatcgcaa agtcgcgcca tcgacgccaa agagatcctg
    32281 atgaagcgca aaatcactgc cgcaccggtg gtagatgaaa acggcaaact caccggcgca
    32341 attaacctgc aggatttcta tcaggccggg attatttaat ccttcattcc cagacgtttc
    32401 gccagccgat gcaggttggc gacgtcggtt tccagcatcc gcgcgcaggc agcccagttg
    32461 tgatgatttt gtgccagcgc ctgacgaata gtttcacgct ggaacgcttc tgtcgcttca
    32521 cgcaggtttt gcttaacaac gggcaccgcc gccacttctg gcgtcggcaa cgtcacctca
    32581 ggaaaagcaa aatgttgcgc ctcaagaatc acttcatcgc cgttgcgggt ggctctcgac
    32641 agcactaccg cccgatgaat agcatgttcc agttcgcgca cgttccccgg aaaacgatag
    32701 tgttgcagta aatttcgcgc tccggcactt aataccacgc gggagagccc cagccgcaaa
    32761 cgacactgct cgcagaaata ccccgccagc agaatgacat catcgccccg ctcacgcagc
    32821 ggcggcaccg aaagtggaaa cacgctcagg cgatgaaaca agtcagcgcg aaatcgccct
    32881 gccagcacct cttcgcgtaa gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc
    32941 aaactgcggt catcgccaac gcgctgaata tcgccatact gcaacaccct cagcagcttg
    33001 gcctgcaatg ccaacgacaa ctcgccgatc tcatccagaa acagagtgcc gttatccgcc
    33061 atttcaaact tcccgctgcg gttactgata gcgccagtaa acgctccttt cacatgcccg
    33121 aacagttcac tttccgccac actttccggc agtgcggcac agttgagata gaccagcgga
    33181 ttcaccgccc gtggcgaggc ttcatgaatc gctttcgcca ccagctcctt accggttccc
    33241 gtctcaccgc tgatcaggac gttgagatcg gacgccgcca caatctcaat ctcttttttc
    33301 agttgcgtca tgccagggga caagccaatc atctgcgtct gtttcaccgc ttcaaacggc
    33361 gtggcatcgc ctggcatcat attctggctt tccagttgtt caatcagcaa cgcattgctt
    33421 aacgctcccg ccgccagcgc agcaatcagc cgtagctctt cgtcgctgaa aacatcgaac
    33481 tgatcgggct gcatcccgtc gagcgtcagt gcgccgatca ggttttgccc ggcaaacaat
    33541 ggcagaccaa cgcaggcgtg aaccttcaga ctctcctgcc caggaatcaa accgtcatag
    33601 ggatcgggca attcgctgtc tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct
    33661 tccagccgtg gatgcccttc cagcgcaaag cgtctaccga gtacatcctt tgccagaccg
    33721 tcgatggcaa gcggaataaa ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac
    33781 tccagcacct gacgtagcgt ggtgatcagg cgctgaaaac gatcctgatg accaatccca
    33841 cgctgcaatt cgatggcgat attcgccagc acatcaacgg aaaaactcat ctttgcctca
    33901 ctgtcaattt gactatagat attgtcatat cgaccatttg attgatagtc attttgacta
    33961 ctcattaatg agcatacttt tatttatata gtaaaaacaa tcagataaaa aactggcacg
    34021 caatctgcaa ttagcaagac atctttttag aacacgctga ataaattgag gttgctatgt
    34081 ctattgtggt gaaaaataac attcattggg ttggtcaacg tgactgggaa gtgcgtgatt
    34141 ttcacggcac ggaatataaa acgctgcgcg gcagcagtta caacagctac ctcatccgcg
    34201 aagaaaaaaa cgtgctgatc gacaccgtcg accataaatt cagccgcgaa tttgtgcaga
    34261 acctgcgtaa tgaaatcgat ctggcggata tcgattacat cgtgattaac catgcagaag
    34321 aggaccacgc tggggcgctg accgaactga tggcgcaaat tcccgatacg ccgatctact
    34381 gtactgccaa cgctatcgac tcgataaatg gtcatcacca tcatccggag tggaatttta
    34441 atgtggtgaa aactggcgac acgctggata tcggcaacgg caaacagctc atttttgtcg
    34501 aaacaccaat gctgcactgg ccggacagca tgatgactta cctgacaggc gacgcggtgc
    34561 tgttcagtaa cgatgctttc ggtcagcact actgcgacga gcatctgttc aacgatgaag
    34621 tggatcagac ggagcttttc gagcagtgcc agcgttacta cgccaatatc ctgacgccgt
    34681 tcagccgcct ggtaacgccg aaaattaccg agatcctggg ctttaactta ccagtcgata
    34741 tgatagcgac ctcccacggc gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt
    34801 acctgaagtg ggccgctgat tatcaggaag acagaatcac cattttctac gacaccatgt
    34861 cgaataacac ccgcatgatg gctgacgcta tcgcccaggg gattgcggaa accgacccac
    34921 gcgtggcggt gaaaattttc aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg
    34981 tcttccgctc aaaaggcgtg ctggtcggca cttcgacgat gaataacgtg atgatgccga
    35041 aaatcgccgg gctggtggag gagatgactg gtttacgctt ccgtaacaaa cgcgccagtg
    35101 ctttcggctc tcacggctgg agcggcggtg cggtggatcg tctttccacg cgcctgcagg
    35161 atgcgggttt cgaaatgtcg cttagcctga aagccaaatg gcgaccagac caggacgctc
    35221 tggagttatg tcgtgaacac ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc
    35281 cgcagagcac ggtgaatacg gtggttaaag aagaaacctc tgccaccacg acggctgacc
    35341 tcggcccacg gatgcagtgc agcgtctgcc agtggattta cgatccggca aaaggcgagc
    35401 caatgcagga cgttgcgcca ggaacgccgt ggagtgaagt cccggataac ttcctctgcc
    35461 cggaatgctc cctcggcaaa gacgtctttg aagaactggc atcggaggca aaatgagtaa
    35521 cggcattgtg atcatcggtt cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa
    35581 acaggacgcc actattccat taaccctgat tgccgccgac agcatggatg agtacaacaa
    35641 acctgacctc agccatgtta tcagtcaggg gcaacgtgcc gatgacctta cccgccagac
    35701 ggcgggtgaa tttgccgagc agtttaatct gcacctgttt ccacaaacct gggtgacgga
    35761 tatcgatgcc gaagcccgtg tggtgaaaag ccagaataat cagtggcaat acgacaagct
    35821 agtactggca accggtgcca gtgcctttgt cccgcctgtg cctgggcgtg agttaatgct
    35881 gacgttaaat agtcagcaag agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg
    35941 cgtgttgatt gttggcggtg gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc
    36001 aggcaaagcg gtcacgctaa tcgacaacgc tgccagtatt ctggcgtcgt taatgccacc
    36061 ggaagtaagc agccgcttgc agcatcggtt gacggagatg ggcgttcatc tgttgttgaa
    36121 atctcagttg caggggctgg aaaaaacaga ttctggcatt ctggcaacgc tggaccgcca
    36181 gcgcagtatc gaagtggatg cggtaattgc cgccaccgga ctgcgcccgg aaaccgccct
    36241 ggcacgacgc gccgggctga cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac
    36301 cagtaatgcc gatatttacg cgctgggcga ttgcgcggaa attaacggtc aggtattgcc
    36361 gttcctccag ccgattcaac ttagcgcgat ggtgctggca aaaaatcttc tcggcaataa
    36421 cacgccactg aaactaccgg cgatgctggt gaaaatcaaa acgccggaat taccgctgca
    36481 tctggcaggc gaaacccagc gtcaggattt acgctggcaa attaataccg aacgccaggg
    36541 aatggtggct cgcggtgttg acgatgctga ccagcttcgc gcctttgtgg tcagtgagga
    36601 tcggatgaaa gaggcatttg gattgttgaa aacattgccg atgtaggtgg gctactgtgc
    36661 ctaaaatgtc ggatgcgacg ctggcgcgtc ttatccgacc tacggggacg catgtgtagg
    36721 ccggataagg cgtttacgcc gcatccggca atggtgtcca aatgcaacac gttttatccg
    36781 ttctggactt cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa
    36841 ccgccatcac ccgccggtaa actctgtgga aagagcaatg tgaaatcagc gagataatgc
    36901 gccagacgtg cacgcagcaa acggttatga ataaccccgc cgctaaatac cagcgtagtg
    36961 ataccacgca tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca
    37021 tgaaacgccc acgcgcgttg attaaccggt gcctgccagt tcagccactg ctgccagaaa
    37081 gtggcgagat ccagttgatt gtccaccagc ggcattgtca ccggatgcgt cactccgtgg
    37141 cacgaagctg cgagtgcctc cagagcacaa gccgcttcac cttcataact taacgtggct
    37201 ggcgcacagc ccagtgccgc cgccacggca tcgaacaacc gcccacacga tgacgccagc
    37261 ggcgcgttaa ttccacgctc aatggcccgc gccagcacgc tccagttttg ctgttgcaca
    37321 cttgctgttt cagaataatt ctgccactcc ggcacaaagc gcaggcactg cgccagcagg
    37381 tttcgccacg gctgcgtcgc cgccaaatcg ccacccggaa gcgccactgc aggcaagccg
    37441 cccaggtgct cacattcgcg atagttcacc cgcagacact cgccgcccca caaagcgccg
    37501 ttctccccca taccgatacc atcgagcgtc aaagcaatga catcaccgcc atccagcggc
    37561 cactgatgct ctgccagaca cgccgctgcg tgggcatgat gatgcagcac cgtttgcgtc
    37621 ggcatattca tttcgctcgc ccactggctg gagacatagc ccggatgcac gtcatgcaca
    37681 acgtattgcg gagtgaaatc gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc
    37741 atctggatgc catcgtcact taaatcgccc agatgctgac tcaacaccgc ttgttcaccg
    37801 cgcaccaggc agaaggtgtt tttcagatcc gcgccaagac acagcacagg cggaacattt
    37861 ttaaagcccg gaggcaaaac cagcgcatcc ggcacatacc cgcgtgaacg gcgcagcatt
    37921 tcgccgcttt cgcgcaccac cgaatcatcc atccgctgca cgatgtcgcg gttatgtatc
    37981 aagaatccgt cggcaatgcc ctgcaaatcc gccagcgcct gttcgttgct gatagctggt
    38041 ggtttaccgc tcaggttgcc ggaggtcatc accagcgggc attgcagttc ctgtaacagc
    38101 aaatgctgga gcgggttcgc aggcaacatt accccgactt cgttaaggtc aggggcgata
    38161 tcatcacaaa gctcaggaac gtatttttta tccaccagca caatcggcgc ggcgggcgtg
    38221 gtaagcaact ggcgcgcagc gtctggtaaa ccgtcagcca ctggcaacat gaccgccagc
    38281 ggtttcgccg ggcgatgttt gcgcgcccgc agtgtcgcca ccgcgttact gttacgtgca
    38341 tcgcaggcaa gatgaaatcc gccaatccct ttgatggcga caatgttgcc catttttaac
    38401 tgtgcgatag ccgcctgtaa tgccgcctct tgttctgcat gttcaccatg acttacccat
    38461 tcaagatgtg ggccacactc cgggcaggcc accggctggg cgtggaagcg acgatcgagc
    38521 gggtcgcggt actctttgtc acaagccgga cacaacggaa acgccgccat cacggtaaac
    38581 gggcggtcgt aaggcatggc gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg
    38641 ataaacggat aacgataacg ccgttcgcct ggggtattca tttcggcaag acaggcaggg
    38701 caagtagcgg catcgggaac aatttgcgta ttcatggtgc cgcctgtgct ctggcgtata
    38761 gtgaactcgg tgggcagttg tgaccagatg tacggctcac gctcgacgct atcaatacgc
    38821 gccagcggcg ggcagtgctg atgcaattga acaagaaacg tttccgggtc ttccagcagc
    38881 cggacttcta cgccatcgcc gtcattacag acatcgccgt gaagatttaa ttgctgtgcc
    38941 agctgccaga caaacggacg aaaaccgacg ccctgcactt tgcctcgaat acgcagttgg
    39001 acaccgcaag atgtgttttt tgccattgag ttattcccgc catcatgaat tgcgtaaccc
    39061 gccctgccgg acacgactgc gtcgtatccg gcagtctcag gtcggcgata ccgccagctc
    39121 cgtattctac gaatatttcc gggaattcct ttgatgccag aacagttcta taagattttt
    39181 agaacatcag cgccgtacgg cgacgttttt ctgcgctcag ttgttcaagt ttattacgat
    39241 cgacacaaat cagcgcatgg gtcgggcaag ccgccataca cgccgggccg tcttcgcgat
    39301 ggttgcacag gtcgcattta ttggcttcag ctttgtcagc ccgtacattc agccccgcgc
    39361 cgctgttgcg gatcaccgga cgtaccacca cttccatcgc gccatacggg caagccacca
    39421 cgcaggtttt gcaaccaatg caacgttcct gcatcacatg aacaaaccct ttatcacggc
    39481 tgatagcacc attcgggcag acgttagcgc acggtgcatc ttcacactga cggcaaactg
    39541 tcgccgtgga aatgttcaca cctttaatga catggatacg cggtaaaaat gtttccgggg
    39601 tcagcgatgc acagtcctga ttttcctgat gagaaaccac gcacgccact tcacaggtac
    39661 ggcaaccaat acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca
    39721 atgacagtta atgcgccgat acattcacaa atcatgccag tttttaattc actgttattt
    39781 aaggaaatta atttctgtaa tgcaggaaaa aagatgtcat cgacactagt gacgatgaca
    39841 tgtgatgaca atgtttatcg cgaaagagca atgagtgagt cgcggcggat cagttttccg
    39901 ctgaaggttt tcggcggtga gaaatccccg ccatcgagca taaaaatcag ccgtccaata
    39961 atttcctgaa tcatctctgt taccggaatt tttacgctgg agagcgccgg aacggtgtag
    40021 ggggcaattg cgatatcatc gaatccgata accgacacct gctctggcac cgctacgccg
    40081 cgctcgtgca acgctttgat cgcacctatc gccatatcgt cgttactggc aactaacgcg
    40141 ctaaatttag ccccacgttc gagcaacgtt tctacccctt cggccccgct ggcgggcgtc
    40201 catttaccgt tagcgataag tttttcattg agcgcaatac catgctgcga cagcgcgtct
    40261 ttatacccgg caagacgttc aacgctggtg ggggaatcca tcgagccggt aaggaaagca
    40321 atctcctgat gtccggcgtt tatcaactct gccacggcat taaagctggt ctgtttatga
    40381 tcgcaccaga cgctatggct gctgtttttg cgcaggcggc gattaagcac cattatcggc
    40441 tgactgtgcg cgtcaatgat gtcatcgatc tcatccacgc ttaaaaaacg cgggtaaatc
    40501 ataatggcgt cgcagcgcaa atccagcaga tactgaatcg cctggcgctc ttcttcggcg
    40561 ctgtgtttac catctgccaa tagcaactgc cgccctttct cttccgccat tcgcgcggca
    40621 tgaaagagta attcactaaa ataaatgccg tggtaaagcg tgttggtcac taccagcccc
    40681 agcgtctgag tactcttcgc cgacagattg cgcgccagca agtttgggcg gtaaccgctc
    40741 tcttctaccg cctgaaacac gcgatcttta gtctcctggc tgacgtagcc attacctgaa
    40801 agcacgcggg aaacggtcgc ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc
    40861 gtcatcattt tcatcccttt acacgcaatc aacgcagtgt actgcaccgt ttgccgattg
    40921 tccttgcgca atcggcggga aaaatattca ggtgaccggt ttcgcaaata taaaaaatga
    40981 acaattaact ctcttgctta tttagtgaca actattcatg attttgtgaa accggtttcc
    41041 taattccgtt tcagcatcgg catttttccg ttacgtcgac tgataacaac tacatctacc
    41101 ctactgataa caggataaaa tccgatggcc aaaaattatg cggcgctggc acgctcggtg
    41161 atagcggcac tgggcggcgt tgataacatc tcggcagtca cgcactgtat gacgcgtttg
    41221 cgctttgtta tcaaagatga ccagcttatc gacagcccga cgttaaaaac catccccggc
    41281 gtgctcggcg tggtacgtag tgacaaccag tgtcaggtga ttatcggcaa taccgtttca
    41341 caagcctttc aggaagtcgt cagcctgctg ccgggagata tgcagcccgc acagcccgtg
    41401 ggtaaaccca aactcacgct acgtcgcatt ggtgcgggga tcctcgatgc gctgatcggc
    41461 accatgtcac cgctgatccc ggcgattatc ggcggatcga tggtcaaact gctggcgatg
    41521 atcctcgaga tgagcggcgt gctgacaaaa ggatcgccga ccttaaccat tctgaatgtg
    41581 attggtgacg gtgctttctt cttcctgccg ctgatggtcg cggcttcggc cgccatcaaa
    41641 tttaaaacca atatgtcgct ggcgattgcc atcgcgggtg tgctggtaca tccgagtttt
    41701 attgaactga tggcgaaagc ggcccagggt gaacatgtcg aatttgccct gataccggtc
    41761 accgcggtga aatacaccta cacggtgatc ccggcgctgg tgatgacctg gtgcctgtca
    41821 tatatcgaac gctgggtgga tcgcattaca ccggcggtga ccaaaaactt cctcaagcca
    41881 atgctgattg tgttgattgc cgcaccgctg gcaatcctgc tgattggccc gattggtatc
    41941 tggatcggta gcgccatttc ggcgctggtt tacaccattc atggttatct gggctggctt
    42001 tcagtcgcca ttatgggcgc gctgtggcct ctgctggtaa tgaccgggat gcaccgcgtc
    42061 tttacgccaa ccatcattca gaccattgcc gaaaccggca aagaagggat ggtcatgccg
    42121 tcagagatcg gcgctaacct gtcgctgggc ggttcatcac tggcggtggc gtggaaaacg
    42181 aaaaacccgg aactgcgcca gacggcgctg gctgcggcgg catcagccat tatggcgggg
    42241 atttccgaac cggcgttata cggcgtggcg atccgcctga aacgtccgct tatcgccagt
    42301 cttatcagcg gttttatttg cggcgcggtt gccggtatgg cggggcttgc cagccactca
    42361 atggcagcgc cggggttatt taccagcgtg caattcttcg atccggcgaa tccaatgagc
    42421 atcgtctggg tgttcgcggt catggcgctg gcggtggtgc tgtcgtttat cctcacacta
    42481 ttgctcggct ttgaggatat tcctgttgag gaagcggctg ccgaggcgcg aaagcatcag
    42541 agcgcacaac cgaccgtcgc caaagaagta agtcttaatt gaggatgaaa atgtcagtat
    42601 ttccagaagg ttttttatgg ggcggcgcgc ttgccgccaa ccagtctgaa ggtgcgttcc
    42661 gtgaaggtgg caaaggactg accacggtcg atatgatccc gcacggcgag catcgaatgg
    42721 cggtgaaact ggggctggaa aaacgttttc agttgcgcga tgacgagttt tatcccagcc
    42781 atgaggcgac ggatttttat catcgttata aagaagatat cgccctgatg gcagagatgg
    42841 gattcaaggt tttccgtacc tcaattgcct ggagccgcct ctttccgcag ggcgatgaaa
    42901 tcacgcccaa tcagcagggc attgcttttt accgttctgt ctttgaagag tgtaaaaagt
    42961 acggtatcga accgctggtc acgttgtgcc acttcgatgt gccgatgcat ctggtcaccg
    43021 aatatggctc ctggcgtaac cgcaagctgg tggagttttt cagccgctac gccagaacct
    43081 gctttgaagc atttgatggt ctggtgaaat actggctaac cttcaatgaa atcaacatta
    43141 tgttgcatag cccgttctcc ggcgcgggtc tggtgtttga agaaggtgaa aatcaggatc
    43201 aggtgaaata tcaggccgcg catcaccagc tggttgccag tgcgctagcc accaaaatcg
    43261 cccatgaggt taacccgcaa aatcaggtgg gctgtatgct ggcgggcggt aacttctacc
    43321 cttacagctg caaaccggaa gatgtctggg cggcgctgga gaaagatcgg gaaaacctgt
    43381 tttttatcga tgtgcaggcg cgcggcgcgt atccggctta ctctgcccgc gtatttcgcg
    43441 aaaaaggggt aaccatcaac aaagcaccgg gcgatgatga aatcctgaaa aacaccgtcg
    43501 attttgtctc tttcagctat tacgcctcgc gctgcgcctc ggcggagatg aacgccaaca
    43561 acagcagtgc ggcgaacgtg gtgaaatcgc tgcgtaatcc gtatctacag gtgagcgact
    43621 ggggctgggg aattgatcca ctcggtctgc gtatcaccat gaatatgatg tacgatcgtt
    43681 atcagaagcc gctgtttctg gtggaaaacg gcctcggcgc aaaagatgaa cttgctgcca
    43741 atggcgagat taacgatgac tatcgcatca gctatttacg cgaacatatc cgcgcaatgg
    43801 gcgaagcgat tgcagatggc attccgctga tgggctacac cacctggggc tgtattgatt
    43861 tagtttccgc ctctacgggt gaaatgagca aacgctacgg ttttgtctac gtagaccgtg
    43921 acgacgcagg caacggcacg ctgacgcgca cgcgtaagaa atcgttctgg tggtataaaa
    43981 aagtaattgc cagtaatggg gaagatttag agtaagtaac cgtgccggat gcggcgtgaa
    44041 cgccttatcc ggcctacata gttcgtgcga
//
LOCUS       DAZFVO020000020        43121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFVO020000020 DAZFVO020000000
VERSION     DAZFVO020000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 43121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.020"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3070)
                     /gene="acrF"
                     /locus_tag="ACHKNB_RS05210"
                     /old_locus_tag="pgaptmp_001042"
     CDS             complement(<1..3070)
                     /gene="acrF"
                     /locus_tag="ACHKNB_RS05210"
                     /old_locus_tag="pgaptmp_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HGU4194602.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPV"
     gene            complement(3082..4239)
                     /gene="acrE"
                     /locus_tag="ACHKNB_RS05215"
                     /old_locus_tag="pgaptmp_001043"
     CDS             complement(3082..4239)
                     /gene="acrE"
                     /locus_tag="ACHKNB_RS05215"
                     /old_locus_tag="pgaptmp_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HGU4194603.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4638..5300
                     /gene="acrS"
                     /locus_tag="ACHKNB_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /pseudo
     CDS             4638..5300
                     /gene="acrS"
                     /locus_tag="ACHKNB_RS05220"
                     /old_locus_tag="pgaptmp_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
     gene            complement(5303..5482)
                     /locus_tag="ACHKNB_RS05225"
                     /old_locus_tag="pgaptmp_001045"
     CDS             complement(5303..5482)
                     /locus_tag="ACHKNB_RS05225"
                     /old_locus_tag="pgaptmp_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HGU4194604.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(5566..6450)
                     /gene="yhdJ"
                     /locus_tag="ACHKNB_RS05230"
                     /old_locus_tag="pgaptmp_001046"
     CDS             complement(5566..6450)
                     /gene="yhdJ"
                     /locus_tag="ACHKNB_RS05230"
                     /old_locus_tag="pgaptmp_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HGU4194605.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6536..6832)
                     /gene="fis"
                     /locus_tag="ACHKNB_RS05235"
                     /old_locus_tag="pgaptmp_001047"
     CDS             complement(6536..6832)
                     /gene="fis"
                     /locus_tag="ACHKNB_RS05235"
                     /old_locus_tag="pgaptmp_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HGU4194606.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6858..7823)
                     /gene="dusB"
                     /locus_tag="ACHKNB_RS05240"
                     /old_locus_tag="pgaptmp_001048"
     CDS             complement(6858..7823)
                     /gene="dusB"
                     /locus_tag="ACHKNB_RS05240"
                     /old_locus_tag="pgaptmp_001048"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGU4194607.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8152..9033)
                     /gene="prmA"
                     /locus_tag="ACHKNB_RS05245"
                     /old_locus_tag="pgaptmp_001049"
     CDS             complement(8152..9033)
                     /gene="prmA"
                     /locus_tag="ACHKNB_RS05245"
                     /old_locus_tag="pgaptmp_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGU4194608.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(9045..10496)
                     /gene="panF"
                     /locus_tag="ACHKNB_RS05250"
                     /old_locus_tag="pgaptmp_001050"
     CDS             complement(9045..10496)
                     /gene="panF"
                     /locus_tag="ACHKNB_RS05250"
                     /old_locus_tag="pgaptmp_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HGU4194609.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10486..10728)
                     /locus_tag="ACHKNB_RS05255"
                     /old_locus_tag="pgaptmp_001051"
     CDS             complement(10486..10728)
                     /locus_tag="ACHKNB_RS05255"
                     /old_locus_tag="pgaptmp_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HGU4194610.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10837..12186)
                     /gene="accC"
                     /locus_tag="ACHKNB_RS05260"
                     /old_locus_tag="pgaptmp_001052"
     CDS             complement(10837..12186)
                     /gene="accC"
                     /locus_tag="ACHKNB_RS05260"
                     /old_locus_tag="pgaptmp_001052"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGU4194611.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12197..12667)
                     /gene="accB"
                     /locus_tag="ACHKNB_RS05265"
                     /old_locus_tag="pgaptmp_001053"
     CDS             complement(12197..12667)
                     /gene="accB"
                     /locus_tag="ACHKNB_RS05265"
                     /old_locus_tag="pgaptmp_001053"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGU4194612.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13645..14619)
                     /locus_tag="ACHKNB_RS05270"
                     /old_locus_tag="pgaptmp_001054"
     CDS             complement(13645..14619)
                     /locus_tag="ACHKNB_RS05270"
                     /old_locus_tag="pgaptmp_001054"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HGU4194613.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14771..16711
                     /gene="csrD"
                     /locus_tag="ACHKNB_RS05275"
                     /old_locus_tag="pgaptmp_001055"
     CDS             14771..16711
                     /gene="csrD"
                     /locus_tag="ACHKNB_RS05275"
                     /old_locus_tag="pgaptmp_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HGU4194614.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17016..18059
                     /gene="mreB"
                     /locus_tag="ACHKNB_RS05280"
                     /old_locus_tag="pgaptmp_001056"
     CDS             17016..18059
                     /gene="mreB"
                     /locus_tag="ACHKNB_RS05280"
                     /old_locus_tag="pgaptmp_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HGU4194615.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18125..19228
                     /gene="mreC"
                     /locus_tag="ACHKNB_RS05285"
                     /old_locus_tag="pgaptmp_001057"
     CDS             18125..19228
                     /gene="mreC"
                     /locus_tag="ACHKNB_RS05285"
                     /old_locus_tag="pgaptmp_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGU4194616.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19228..19716
                     /gene="mreD"
                     /locus_tag="ACHKNB_RS05290"
                     /old_locus_tag="pgaptmp_001058"
     CDS             19228..19716
                     /gene="mreD"
                     /locus_tag="ACHKNB_RS05290"
                     /old_locus_tag="pgaptmp_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGU4194617.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19725..20318
                     /gene="yhdE"
                     /locus_tag="ACHKNB_RS05295"
                     /old_locus_tag="pgaptmp_001059"
     CDS             19725..20318
                     /gene="yhdE"
                     /locus_tag="ACHKNB_RS05295"
                     /old_locus_tag="pgaptmp_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HGU4194618.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20308..21777
                     /gene="rng"
                     /locus_tag="ACHKNB_RS05300"
                     /old_locus_tag="pgaptmp_001060"
     CDS             20308..21777
                     /gene="rng"
                     /locus_tag="ACHKNB_RS05300"
                     /old_locus_tag="pgaptmp_001060"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HGU4194619.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21845..25645
                     /gene="yhdP"
                     /locus_tag="ACHKNB_RS05305"
                     /old_locus_tag="pgaptmp_001061"
     CDS             21845..25645
                     /gene="yhdP"
                     /locus_tag="ACHKNB_RS05305"
                     /old_locus_tag="pgaptmp_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HGU4194620.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25801..27246
                     /gene="tldD"
                     /locus_tag="ACHKNB_RS05310"
                     /old_locus_tag="pgaptmp_001062"
     CDS             25801..27246
                     /gene="tldD"
                     /locus_tag="ACHKNB_RS05310"
                     /old_locus_tag="pgaptmp_001062"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HGU4194621.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27374..28303)
                     /gene="aaeR"
                     /locus_tag="ACHKNB_RS05315"
                     /old_locus_tag="pgaptmp_001063"
     CDS             complement(27374..28303)
                     /gene="aaeR"
                     /locus_tag="ACHKNB_RS05315"
                     /old_locus_tag="pgaptmp_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HGU4194622.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28486..28689
                     /gene="aaeX"
                     /locus_tag="ACHKNB_RS05320"
                     /old_locus_tag="pgaptmp_001064"
     CDS             28486..28689
                     /gene="aaeX"
                     /locus_tag="ACHKNB_RS05320"
                     /old_locus_tag="pgaptmp_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HGU4194623.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28697..29629
                     /gene="aaeA"
                     /locus_tag="ACHKNB_RS05325"
                     /old_locus_tag="pgaptmp_001065"
     CDS             28697..29629
                     /gene="aaeA"
                     /locus_tag="ACHKNB_RS05325"
                     /old_locus_tag="pgaptmp_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HGU4194624.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29635..31602
                     /gene="aaeB"
                     /locus_tag="ACHKNB_RS05330"
                     /old_locus_tag="pgaptmp_001066"
     CDS             29635..31602
                     /gene="aaeB"
                     /locus_tag="ACHKNB_RS05330"
                     /old_locus_tag="pgaptmp_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HGU4194625.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31694..31966
                     /locus_tag="ACHKNB_RS05335"
                     /old_locus_tag="pgaptmp_001067"
     CDS             31694..31966
                     /locus_tag="ACHKNB_RS05335"
                     /old_locus_tag="pgaptmp_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HGU4194626.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(32022..32285)
                     /gene="yhcN"
                     /locus_tag="ACHKNB_RS05340"
                     /old_locus_tag="pgaptmp_001068"
     CDS             complement(32022..32285)
                     /gene="yhcN"
                     /locus_tag="ACHKNB_RS05340"
                     /old_locus_tag="pgaptmp_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HGU4194627.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32650..33120)
                     /gene="argR"
                     /locus_tag="ACHKNB_RS05345"
                     /old_locus_tag="pgaptmp_001069"
     CDS             complement(32650..33120)
                     /gene="argR"
                     /locus_tag="ACHKNB_RS05345"
                     /old_locus_tag="pgaptmp_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HGU4194628.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33555..34493
                     /gene="mdh"
                     /locus_tag="ACHKNB_RS05350"
                     /old_locus_tag="pgaptmp_001070"
     CDS             33555..34493
                     /gene="mdh"
                     /locus_tag="ACHKNB_RS05350"
                     /old_locus_tag="pgaptmp_001070"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4194629.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(34556..35623)
                     /gene="degS"
                     /locus_tag="ACHKNB_RS05355"
                     /old_locus_tag="pgaptmp_001071"
     CDS             complement(34556..35623)
                     /gene="degS"
                     /locus_tag="ACHKNB_RS05355"
                     /old_locus_tag="pgaptmp_001071"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HGU4194630.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(35713..37080)
                     /gene="degQ"
                     /locus_tag="ACHKNB_RS05360"
                     /old_locus_tag="pgaptmp_001072"
     CDS             complement(35713..37080)
                     /gene="degQ"
                     /locus_tag="ACHKNB_RS05360"
                     /old_locus_tag="pgaptmp_001072"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HGU4194631.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(37234..37632)
                     /gene="zapG"
                     /locus_tag="ACHKNB_RS05365"
                     /old_locus_tag="pgaptmp_001073"
     CDS             complement(37234..37632)
                     /gene="zapG"
                     /locus_tag="ACHKNB_RS05365"
                     /old_locus_tag="pgaptmp_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HGU4194632.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            37826..38953
                     /gene="zapE"
                     /locus_tag="ACHKNB_RS05370"
                     /old_locus_tag="pgaptmp_001074"
     CDS             37826..38953
                     /gene="zapE"
                     /locus_tag="ACHKNB_RS05370"
                     /old_locus_tag="pgaptmp_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HGU4194633.1"
                     /translation="MQSVTPTSQYLKELNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            39172..39600
                     /gene="rplM"
                     /locus_tag="ACHKNB_RS05375"
                     /old_locus_tag="pgaptmp_001075"
     CDS             39172..39600
                     /gene="rplM"
                     /locus_tag="ACHKNB_RS05375"
                     /old_locus_tag="pgaptmp_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGU4194634.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            39616..40008
                     /gene="rpsI"
                     /locus_tag="ACHKNB_RS05380"
                     /old_locus_tag="pgaptmp_001076"
     CDS             39616..40008
                     /gene="rpsI"
                     /locus_tag="ACHKNB_RS05380"
                     /old_locus_tag="pgaptmp_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGU4194635.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            40403..41041
                     /gene="sspA"
                     /locus_tag="ACHKNB_RS05385"
                     /old_locus_tag="pgaptmp_001077"
     CDS             40403..41041
                     /gene="sspA"
                     /locus_tag="ACHKNB_RS05385"
                     /old_locus_tag="pgaptmp_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HGU4194636.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            41047..41544
                     /gene="sspB"
                     /locus_tag="ACHKNB_RS05390"
                     /old_locus_tag="pgaptmp_001078"
     CDS             41047..41544
                     /gene="sspB"
                     /locus_tag="ACHKNB_RS05390"
                     /old_locus_tag="pgaptmp_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HGU4194637.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(41587..42954)
                     /gene="dcuC"
                     /locus_tag="ACHKNB_RS05395"
                     /old_locus_tag="pgaptmp_001079"
     CDS             complement(41587..42954)
                     /gene="dcuC"
                     /locus_tag="ACHKNB_RS05395"
                     /old_locus_tag="pgaptmp_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4194638.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIHSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMELVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT        10527 a        10793 c        11221 g        10580 t
ORIGIN      
        1 acaccggtac gaagaagatt gccagcaacg ttgcagagac cattcctccc attaccccga
       61 tacccacagc gttctgcgcg ccactgccgg caccgttact gatagctagc ggtaatacgc
      121 cgagaataaa ggcgagagag gtcatcagga taggacgcag acgcatacgt actgccatca
      181 gtgtcgcttc aacaacacct ttaccctctt tctccatgag atctttagcg aactcaacaa
      241 tcaaaatagc gtttttggcc gacaagccaa ttgtcgttag caagcccacc ataaagtaga
      301 cgtcattttt ttgattaaag agtgttgccg ccagtagcac gccgacaatc cctaacggca
      361 ctaccaacat aaccgagaca ggaattgacc agctttcata gagtgcagca aggcacagga
      421 aaacaaccac aaaggaaatt gctaccagag cgggagcctg gtttcccgat aagcgttcct
      481 gatacgacat acccgtccag tcataaccaa tgcccgcagg taattttgac gcaaggtttt
      541 ccatcaacgc catggcatcg ccggaactgg ttcctggcgc ggcttccccc tgaatctcca
      601 ttgacggcag accgttgtag cgttccagtc gcggagagcc atacacccaa tgtgaagtgg
      661 taaaggccga gaatggcacc atttcgccgt tggcgctgcg gacataaagt ttatcgacat
      721 cttctggcag catacggaat ttggcatccg cctgaacata cacctttttc acgcgaccac
      781 ggtcgatgaa gtcgttaacg taagtcccac ccaacgccgt tgaaatggtc tgattgatgt
      841 cagaaagtga aacacctaat gcctgcgcct tttcctggtc aacttccagt ttaaactgcg
      901 cggtgtcttc caggccatta ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca
      961 tgccaagcaa ctggttacgg gcctgggtca gggcatcgtg acccagccca gcctgatcaa
     1021 ttaactcaaa gtcgaaaccc gttgccgtgc ccagttcaac aatggctggc atattgaatg
     1081 gaatgacaaa accgtcgcgg atcttgccca attccatttt ggcacgatgg attaccgctt
     1141 ccgcactgtt ttcatcacta ttacgctctt cccacggttt cagactgacg aaggccatac
     1201 cggcgttttg tgcttggccg ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt
     1261 tcgctttctc gttcttcaga tagtaatccg tcacttgatc caacactttt tgcgtccgct
     1321 cttgcgtcgc gccagcgggt aactgaatca tggtcagaaa gacaccctga tcctcttcag
     1381 gtaagaagga agacggaaga cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat
     1441 agatcagtaa atatcgtcct gtggaaccga ggattttgcc gacgctgttg gtgtagtggt
     1501 taacgctatg atcgaaggtg gtattaaacc agccgaagaa accaccctta ttttcgtgat
     1561 gctcagctga ggtgggtttg agcagtgttg cacataacgc aggggtaaga atcaatgcca
     1621 ccagaacaga aagcgccatt gccgaaacga tggtgataga gaactggcga taaattgccc
     1681 cagtagaacc gccgaagaat gccatcggaa taaataccgc tgacagcacc atcgcgatac
     1741 ccaccagtgc gccctgaatt tgcgacatcg atttttccgt cgcttctttt ggcgggagct
     1801 tatcctccat catcacgcgc tcgacgttct ccaccaccac tatcgcatca tcgacgagca
     1861 gcccgatggc aagcaccatc ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg
     1921 cgaggatggc aaacgtccct aataacacca cgggtaccgc aatggtgggg atcagcgttg
     1981 ctcgcatatt ctgcaagaac agatacatca ccaggaacac cagcataatg gcttcgaaca
     2041 gcgtttttac cacttcgtga atagaaagct ggacgaatgg cgtggtgtca taagggtaga
     2101 gaaccttcat tccctgcggg aagaatggct gtaattccgc cagttttgcc ttaatggctt
     2161 tcgcggtatc gagagcattc gcgccggttg ccagcttaat ccccaggccc gccgccggtt
     2221 ttccgttgat acgagcgata acgttatagt tttcaccgcc aagttcaact cgtgcgacat
     2281 ctttcaggcg taccaccgag ccgtcactgt ttacgcgcag ggttactttg ccgaattctt
     2341 ccggattttt taaccgcgtc tgagcaataa ttgaggcgtt caactgttgc cctggtaacg
     2401 ctggcgttcc gcccaactgt ccggcagcga tctgatcgtt ctgtaccttc aactggttaa
     2461 tcacatcaac cggtgtcagt ttatatttgt ttagcagatc ggcgtccagc cagatacgca
     2521 tcgcatactg tgcgccgaaa agctgtacgt caccgacgcc attcagacgg ctaagcgtat
     2581 ctttaacgtt agaggccaca tagtccgaga tatcgtcctg tgtggtgtct gggttatcag
     2641 agacaaagcc cgccaccatc aaatagctgc tactggactt ttcaacactg atcccctgct
     2701 gctgaacctc ctgcggcagc aacggcgtgg cgagctggag tttgttctgc acctgcactt
     2761 gcgcgatatc aggatcggtc ccggactgga aggtaagggt aattgtcacg ctaccggcgg
     2821 aatcgctggt ggaggacata tacatcaggt tatcgatacc gttcatattc tgttcgataa
     2881 cctgcgtcac cgtatcctgc acggtctgcg catcagcgcc cggatagttt gctgaaacag
     2941 aaaccgcagg cggtgcaatg gttggatact gagcgacggg taattgtagg atcgccagtg
     3001 cgcccgccat catcagaata atggccagca cccatgcaaa tatcggtcgt cgaataaaaa
     3061 agtttgccat gtcagattac cttacttcga tgcagtatct gcgggggtat cggtagtggc
     3121 tttaacctgc tctcccggac gcgctttttg caggccgctg acaatgactt gatcgccaga
     3181 tttcagacct tcgctaatca accatttatc gccaatcgcc tgactggcaa cgacagggcg
     3241 cgcttccacc tgacttttat cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg
     3301 gctaacgcct tgttgcggga taagaatggc gtcaggttgg acgccttcat caatccgtgc
     3361 gcgcacaaac atacccggca aaagcgtatg ttgcgggtta gggaagacgg cacgtagggt
     3421 tatggagccg gtgctttcat caacggtcac atcagagaat tgcagcgtac ctttcagggg
     3481 ataggtttga ccgttttcca tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa
     3541 atttccttgc tctacggatt gcttcagcct cataaaatcg ttgctggatt gggtcacatc
     3601 aacgtagata ggatcgagct gctggacagt cgccagttca gtcgtttgcc cattagtgac
     3661 aagagcgcct tcggtcacgg tcgatttgcc gatacgtccg ctaattggcg cagtcacttt
     3721 ggtataagcg agattgatgc gagcgctttc gactgtggct tttgcggcaa tcacggcggc
     3781 atcggcctgg cgagcatcag caatggcctg gtcgtactcc tgctggctga tgtatttcgt
     3841 acccacgagc ggaacgtaac gttttaccgt caaatgcgcg atggcggcgg cggcttcact
     3901 tttcgccagt tcgcctttcg cgctgtcata atttgcctga taggtcgcgg gatcgatctg
     3961 gtacagggac tggcctgctt gtacatcgct gccttcagtg aaattacgat tcagtacgat
     4021 cccgctaacc tgtgggcgaa cttcggctat acggtaagca ttggtgcggc ctggtaattc
     4081 agtcttaact tctaacgggg ccgttttcac aatatgaacg gtaacctgcg gttcaccgac
     4141 gtgagctttc tcttctccct tatcgttaca accggcgatt aaagccgcgg agatcagaat
     4201 aaaggagggc aggaggaaaa acctggcatg tttcgtcatt actattcctc aaaaaaccaa
     4261 aagcgcgtta tttacccaga aaggcaacgc gttaactcgc agaaagaaaa atacagtttg
     4321 ctatcctaca aattatcatt cgtcgatgta aggaatagtt atgaatactg gcgtctcaag
     4381 gcacataaac acaaaaaaag attaatattc tactgtttta ttttgacgcg ggttgaaaga
     4441 ggcagaatta aaacctcgta aattgaaata tatattcatg tggcgaatgt attttaggta
     4501 aataatatat attatttatt tgacgattta tagcaactca atattagcct ctcgtataaa
     4561 tacacattag gtgatagatt aaccttcgct attttctcac tctgtgtcga atatatttat
     4621 ttcctgaata attaatcatg gcaaaaagaa ccaaagccga agctctgaag acccggcaag
     4681 aactgattga aactgccatc gcccagtttg cgcagcatgg cgtaagcaag acgacactca
     4741 acgacattgc cgacgctgct aacgttacgc gtggcgctat ctactggcac ttcgaaaaca
     4801 agactcaact gtttaatgag atgtggttgc aacagccttc attgcgggag ttaatccagg
     4861 aacacttgac ggctggatta gagcatgacc cgtttcaaca attgcgtgaa aaattgattg
     4921 tcggcttgca atatattgcc aaaattcccc gctagcaggc gttgctgaaa atcttatatc
     4981 acaaatgtga atttaatgat gagatgctgg ccgagggagt gatacgcgaa aagatgggct
     5041 tcaatccgca gactctccgc gaagtattgc aggcgtgtca gcaacaaggt tgtgtagcaa
     5101 ataacctcga tttagatgtt gtgatgatta ttattaatgg tgccttcagc ggaattgttc
     5161 aaaactggtt aatgaatatg gcgggttatg atctttataa acaagccccc gctctggtcg
     5221 ataacgtatt aagaatgttc atgccagatg aaaacataac gaaattaatt catcaaacga
     5281 atgaattaag tgtcatgtaa attcacattc tgtcctgaaa attcagttcg tcacagttaa
     5341 ccagttttag tggattaact gagttcatat tgcggcattt gtctgtttct gttgccagca
     5401 gttcaatcca ctgcatcagt aaagtatcaa acagaaaaac gaagacagcg aaaacgacga
     5461 gccaccaata cttgcgaatc attacctgag cctgtgtatg tttaaccaag tctggcagga
     5521 aatatacacg aaatgaataa tctgaaaacc aggcttacac ttcctttact ttgcaatgag
     5581 atcggggtca acttcgctta accggcttcg ttttgacagg ttgccggtct ttcttttttt
     5641 cactttcgcc agttcttccg cagagtaatg cgacgcgaca tccagccgtc gaagccccat
     5701 tttgatgtac tcgctgttga tctcaatacc aatgaatttt cgcccgctgg agacggctac
     5761 ggcaccggta gtaaagctac cggcaaacgg gtcgagaacg atatcgcctg ggttggaaga
     5821 ggcgagaata atgcgtttca gtaaggcttc cggtttttgc gtcgggtggt tctcatattc
     5881 atccatcaaa taacgcacgc gcggaaaatc ccagacgtta cccggtactt tttgatgatt
     5941 gtatggctgt ggaggatttt tgcgataatc gatcaacgcg cgctgcgatc cggttttggc
     6001 ttctaccaga atagcatcac cgttgaatgt gtagttcttt gcgtctttca ccatcatcag
     6061 gatgggttcg tacatggagc cgtagtgttt tttcgcctgt actccagaac tgtcatatga
     6121 ccagacgatg cgacttttga tggtaaaaag cttgcggcac tggagatcga taaagggcat
     6181 gttttccgtg ctgttcatga tgtacatgct gccctgcttt ttcagaacgc ggtggcactc
     6241 tgcaatcact tcaaacagcc agtcgataaa cagatcttct ttccaggctt cgatcagacc
     6301 atcaaaattt ttaccgatgt tatatggtgg gtcggcaaag atcagatcga cactttcggc
     6361 ggggatcttt ttaagttcgg caagcgcatc accgtgaata atggtcttag cttcattacc
     6421 aaaccgggtc ggttcacatc ctgttctcat ggtcactccc tttgtgacac ctataaaaaa
     6481 ggcgcttccc catgccgagt agcgcctttt taatcaagca tttagctaac ctgaattagt
     6541 tcatgccgta ttttttcaat tttttacgca gcgtaccacg gttgatgccc atcatcagcg
     6601 cagcacgggt ctggttacca cgggtgtatt gcatcaccat gtccaacagg ggctgttcta
     6661 cttcagccag taccagctca tagaggtcat tcacatcctg accattcagt tgagcaaaat
     6721 agttcttcag tgcctgttta accgagtcac gcaggggttt ttgggttacc tgatcctgag
     6781 agttaacggt agaaacggtc agtacgtcag aatttacgcg ttgttcgaac atagttctgt
     6841 cagctcttta tttctgttta cgcaaaattt tcgaagtatg cctccaacgc ctccagctgt
     6901 tcgctggcat cctcaatggc gttgaatgtg cgccgaaact ggtcatttgg agcgtgttcc
     6961 tggagatacc aggaaacgtg tttacgtgca attcggtacc cttttgccgg accataaaag
     7021 tcatgcagtt cccgaacgtg cgcgcaaagc aagcgcttaa cctctgccaa aggcagcggg
     7081 ggcagcaact ccccagtgtc cagataatgc tggatttccc gaaagatcca gggtcttccc
     7141 tgagctgcgc ggcctatcat cagggcatcc gcccctgtat agtcgagcac agctctggct
     7201 ttaagcgggt cagtaatgtc gccattcgcg ataaccggaa tggaaacttt ctgcttaact
     7261 gcccgaatac tgtcgtactc agcttctcca ttgaacaaac aggcgcgtgt acggccatga
     7321 atggtcagag cctgaatgcc acagtcttca gccagttggg caatctcttc gcagttacgg
     7381 tgttccggtg cccagccggt gcgaatcttc agggtaacag gaacgtccac tgcattgacg
     7441 acctcggtaa ggatcgattt aacgacatcc gggtactgca agagggctga acctgcgagc
     7501 ttgcgattca cttttttagc cgggcaaccc atattgatat caataatctg ggcaccgctt
     7561 tccacgttaa tacgtgctgc atctgccatt tctttcggat cgctaccagc aatttgcacg
     7621 gtgcgaatac cgggttcatc aatgtgcacc atccgtaaac gagatttgtc gctttcccaa
     7681 acctgtgggt tagaagacat catctcggat actgtcaatc cggctcccat ctcgtagcac
     7741 aacgtccgaa aaggtctgtc tgtaatgcca gccatgggcg ctgcgatcag gcgatttctg
     7801 agctgatatt gtccgatgcg catgagttaa gaaatgacca tactgtgact gcaaggcggc
     7861 gtatattacg cattttttgc acgagatgaa aggccaaact ttgaccaatc ctctgagatg
     7921 gatcaaagaa ttgcatttaa aatgagcgtg gtgcgataat tactcataaa aatcatcata
     7981 ttagaaaata gtgactaaaa tttacactca aagaaatttg agtaagttct caatttttct
     8041 ttatgaatga aaattttggc acgcaaattt gcgtaaataa tcggcaaatt tacgtgcctt
     8101 ttgtgagctt gctcgcactt cgccccgcgt caccctacgg cgatgcgaag gttaattctt
     8161 acgaccggta atacggcacc actcttcttt ttccacgacc gggtccagtg cgaagctgtc
     8221 ggcataagct tcacaaacgc tctctgcctg gcttgccaga ataccggaaa ggcccagcaa
     8281 accgcctgaa accggcagga cgctgattaa cggtgccagt tcacgtaatg ggcctgcaag
     8341 gatgttagcg accaccacgt cggctttcat ttcttctggc tgatctttcg gtaagtagag
     8401 ttccagacgg tcagaaacgc cattacgttc ggcgttgtcg cggctggcct gaatcgcctg
     8461 cggatcgata tcaataccaa tggcttttgc tgcacccagt ttcagcgccg cgatcgccag
     8521 aatgccggaa ccacagccaa agtcgatgac tgttttaccg gttaaatcga ggctgtcgag
     8581 ccattgcagg cacagagagg tggttggatg ggtacccgta ccaaacgcca gccctggatc
     8641 taacatcacg ttgacggcgt tttcgtccgg cacatcacgc cagctagggc agatccacag
     8701 tcgttcacca aagcgcatcg ggtggaaatt atccatccat tcgcgctccc agtctttatc
     8761 ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc agcggatggt tttccagaat
     8821 cgccaccacg tcgttcatat cggtttcagc gtcaaacaga ccaatcacat cggtgtcgcc
     8881 ccacaggcgc gtttcgcccg gcagcggctc aaatactggc gtatcgtggg tatcctgaaa
     8941 agtgatagaa acggcacccg cttccatcag cgcatcgcta agatcttccg cgttcgcgcc
     9001 ggtggtgttc agtttcagtt ggatccaagg catggcaaaa ctctttattt atcagtagtc
     9061 aaaacggtag cttgcgggac ggatgtaccg aaacggtttc cgaccaggaa agccagcaaa
     9121 cttagtagta acgagggcac gatagggtgg aagcccaggt actgaatatt cagcgtcgcg
     9181 agtacggcat acagcacgcc gccaacgatc atcgcactta gcgcgccttt ggcgttggcg
     9241 cgttcccagt aaagacccag caccagcggc cacaggaaaa cggcttccag cccaccgaag
     9301 gccagcaaat tcagccagat gatcatttct ggcggcttcc aggcggcaag cagcagcaac
     9361 gcgccgagaa ctaacgtaat taccgccgac atccgcttca gacgcgtctc gttttgcatt
     9421 tgatccggac ggatattcag atagagatct ttaatgatcg tagcggaact ttgcagcaac
     9481 tgggcattga tcgtcgacat gatcgcagcc ataggtgcag ccaggaagat tccggcagca
     9541 aacggtggca gcacttttac cattaacgtc gggatcacca gatccggtac ggtgagatcg
     9601 gggatcactg cacgacctaa cgctccggcc aggtgcatac cgaacatcag gattgcgact
     9661 acaatcgtac cgatgatgat cccccggtgt actgctttgc tgtctttata agagatacag
     9721 cgcaccgcag tatgcggcag gccaatcacg ccaaagcaca ccagtaccca gaacgacgtc
     9781 ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg taaccagttg cggatcgatg
     9841 gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg catgtaccac gccaataagc
     9901 agcacaacgg tgccaatcag catcacaagc ccttgcatgg tgtcgttcag cacgctggcg
     9961 cgaaagccac caaaggcggt atataacgca atgctgatac caaaaatcag cagcccggtt
    10021 tcataaggaa tacccgccgc ggtttccagc aggcgcgcac cgccgataaa ctgcacggtc
    10081 atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca gccacaccag aagacgactc
    10141 tggtagcggg caaacagcat atcgttcagc gtcactgcat tgtagcggcg cgcaagaatc
    10201 gcaaacttct tgccgagaat accgagtgaa agccagactg caggaagctg aatcatcgcc
    10261 agcaataccc agcccagccc gtatttataa gcagctcctg gcccgccgat aaacgaactg
    10321 gcactgatat aggtcgcggt gagcgtcatc gccagcacaa taccgcccat agagcggctg
    10381 ccgaggaaat actcattaag gaaggtgccg gtgctccgtt tacgcatcgc ataaaccgag
    10441 ataccgaaca ccaccaccag ataggcgacc agcggtagaa ttacttcaag ctgcatcgtc
    10501 atcctccagt gggatatcgc gatagataaa tttcaccatc gcccagcaca gtccaataaa
    10561 cagcagcggc gtcaggatgc aggccatctc aaaccagcgc ggaaagccgg taaaaccggg
    10621 ggcaacgcca ggtaagtaag cggctactaa ccataccgcc agatacaaaa gggtcagccc
    10681 cagcgcccag cgcgcctctt tatgggcctg aacaaaacga gtgtccattt tttgtccctg
    10741 atgggtgaag aaagcgagga ttgtacctta tgggggttgt cgatccccag taataaaaaa
    10801 ggccggaaaa tccggccttt tgacgctttt gcagtcttat ttttcctgaa gaccgagttt
    10861 tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat tcatgatgcg
    10921 gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc agctcctgca gcgcattctt
    10981 catgcgggca atcgccacgt cacggttttc accgtagcaa atcagcttac cgatcattga
    11041 gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac gcactccaaa
    11101 accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg tgttcggatc
    11161 ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt cttgcttgat
    11221 cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa cgccggtgat
    11281 catttcagta accgggtgtt ctacctgaat acgggtgttc atttcgatga aatagaactc
    11341 gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat caacacacgc
    11401 tttagcgcaa cgttcgccga tgtagcgacg cagttccggg gtaatgcccg gtgctggtgc
    11461 ttcttcgacc actttctggt ggcggcgctg catggagcag tcacgttccg ccagatagat
    11521 agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat tttccaggta
    11581 tttctccatg taaaccatat cgttgctgaa agcagctttc gcttccgcac gggtcatgga
    11641 gatggattgt gccagttcag cgtcgccgcg cactacgcgc ataccgcgac caccgccgcc
    11701 gccggaggct ttgataatca ccggataacc aatgcgttta gcaatggcac ggtttttatc
    11761 catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa gggacgcccg cttttttcat
    11821 cgccgcgatt gcggatactt tgtcgcccat caggcgaatg gtttctgctt tcgggccaat
    11881 gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg agaggaagcc
    11941 gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgcttatga ttgccgggat
    12001 gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat ctgccagtaa
    12061 tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg acagtcttga tgcccagttc
    12121 tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa caattttatc
    12181 cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct accggttgtc
    12241 cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca
    12301 ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga
    12361 tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggagcgta
    12421 cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg gtcgccggag
    12481 cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct tgttgcatca
    12541 cagggaaact tgcggcagga gctgcacggc tgatgcgtac tgactcttcg ccttcggaga
    12601 tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta atcttacgaa
    12661 tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc
    12721 gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag caggccggga
    12781 cgccggtcta ttttgcccca gagtgtcaat gttagacttg acggacattg tgcagcgatc
    12841 tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt caccttttcc
    12901 gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat tataacgatt tcgtagcaat
    12961 tggcagctaa atactggtct tatcagggaa gataatcaac agctaacatg taaataacct
    13021 tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc atcatcgacg
    13081 gaacttctta taacgtaagg tgaaaagctg taaagccagc cctgcgaaga tgaggggctg
    13141 caatgagata atcttcacag accacaaata atgtagggat gccaggatcg cgacaagata
    13201 gacgacatta cgcagcggtt gctggggatt acccaatttt cactgcatcg tctggataga
    13261 tgtgaatgct aaagcacgca aaatcaaccg actgacaatt ctcagcatta aataggttcg
    13321 agtggttaat ttgtagtcaa ataaaatcag attattcacc ccatgggacc agaaatcagt
    13381 aacaggttaa gcgtagtgtc gcccaggcaa agcaccataa acctaacagg cgacggaggc
    13441 atatcaataa tggctgttta gaggagtatc aatgcgcatc aatttgtggt cagtcgaaat
    13501 catgactttc cagtttggat gatgttgtac aacttcctga ccaaggtgtt cgacgaaagc
    13561 tgtccggcta accgcttttc ccgttaaccg attgaataat gatgtcaaaa tgagttccct
    13621 ctcttattat tccttgctaa atggttagtt aaccttcacc agcgtgcgac cctggatctg
    13681 gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct cttttgccgc
    13741 ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt gtgcgcggcg
    13801 ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca gaataaatgg
    13861 catgaccgtg gttggcagag taaaaccacc cgccagacca caggccgcca cgcagccgcc
    13921 gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt caattgcccc
    13981 agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag ggagaatacg
    14041 actggcacct aaacttttca gatattcatg ggtactttcg cgaccggaaa cggcaacgac
    14101 ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac cactggcacc
    14161 cgtcacgaca atctccccgt cctgcgggcg aacaccggca tcttccagcg ccatcacaca
    14221 cagcatggcg gtaaaaccgg cagtaccgat aatcattgct ttacgcgcgt ccagcccttg
    14281 cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc caccccagtg
    14341 gttttcacca acgccccagc cagtgagtaa cacctcctga ccggcatgaa aacgcggatc
    14401 ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa aattacggat
    14461 gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg accagtgaac
    14521 atcaaccgtg acatcgccct ccggcaggcg actttcgtca agtgtctgta ccgatgcgag
    14581 agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc atgtgcatga
    14641 tggattggaa aataatttct gaagactata ctcgctaatg gaaaagcaaa gccgatgaag
    14701 cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca ctatcggagt
    14761 taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc tcaccgggtt
    14821 aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcaa ttcagtataa
    14881 gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg tgtcgaatga
    14941 cttcagcaca ttaaggccac aaattaccga attaatgatg tcggcagata tcgttcgtgt
    15001 agacctgctc catggtgata aacaggttta taccctggcc agaaatggta gttatcgtcc
    15061 ggttggctcc agcgatctgt ttcgcgaact gagtgttccg ttgataaagc atccggggat
    15121 gtcgctgcgt ctggtttatc aggatccgat gggcaactat ttccattctt taatgactac
    15181 cgcgccgctc acgggggcga ttggctttat cattgttatg ctctttctgg cggtacgctg
    15241 gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc gtatcttaaa
    15301 cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca gaaccagcag
    15361 tgcgctagat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca gccgtcttga
    15421 tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc ggcctcaata accgactctt
    15481 tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta cccacgggat
    15541 cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacttggg ggcacagcca
    15601 ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga tgcgctaccc
    15661 tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac cgcaccggac
    15721 gttaaaagag gcagagagca tcgccggtca gttaatcaaa gccgtcgata ccttgccgaa
    15781 caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgcgcct ggcgtagtgg
    15841 tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg cgggattgca
    15901 gggcggcaat agctgggcta tttacgatga ctcgttgcct gaaaaaggac gcggtaatgt
    15961 tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc tttatcaaaa
    16021 accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc gcatcttcga
    16081 tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt ttggcttatc
    16141 ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt actggccaga
    16201 ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt ttcagcgttg
    16261 gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa ttattgaact
    16321 tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc gtttagtgaa
    16381 tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa gcaccagttg
    16441 gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca gaaacattga
    16501 gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct ccgggaccag
    16561 cacccaagtt tacgccaccg gtgtgcgttc gcgaagcgag tggcagaccc tgattcagcg
    16621 cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg atactaacgt
    16681 gaaaaaatat tcacaaagat actcggttta acctgccgtt taatccgttt tcacgtagaa
    16741 taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt tgcagcacat
    16801 atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg atgcattaag
    16861 cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta attttcaccg
    16921 tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta agcggatttt
    16981 cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt cgtggcatgt
    17041 tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat gtaaaaggac
    17101 aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt gccggttcac
    17161 cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt acgccgggca
    17221 atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc gtgactgaaa
    17281 aaatgctcca gcacttcatc aaacaagtgc acagcaacag ctttatgcgt ccaagcccgc
    17341 gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca attcgtgaat
    17401 ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg gctgccgcaa
    17461 ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat atcggtggtg
    17521 gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct tccgtgcgca
    17581 ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat tacggttctc
    17641 tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct tacccgggcg
    17701 atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt ccacgcggtt
    17761 ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc ggtattgtga
    17821 gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac atctccgagc
    17881 gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt ttgttaatgg
    17941 aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg gcgcgcggtg
    18001 gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc gaagagtaat
    18061 cggatgcagg caggggaaat gtctgtttac cctgcctggt ctgatacgag aatacgcata
    18121 acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta ttctggcggt
    18181 gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca gtcaaatccg
    18241 tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc ctcgtgaatt
    18301 gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg aaaaccgggc
    18361 gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac aatacaaaca
    18421 ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg agcagaaaat
    18481 ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg ttatcgataa
    18541 aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag gtgttgttgg
    18601 tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg atgcgaccca
    18661 cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg gtaacggttg
    18721 tacggatgat ttgcagcttg agcatctgcc agcgaatacg gatattcgtg ttggtgatgt
    18781 gctggtgacc tccggtctgg gcggtcgttt cccggaaggc tatccggtcg cggttgtctc
    18841 ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt caggcgcgtc cgactgcagg
    18901 actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg gcgctaaccc
    18961 gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga tgatgccgca
    19021 ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccggcaa cggggatcgc
    19081 tcagccgact ccgcagcaac cgacgacagg aaatgcagct actgcgcctg ctgcgcccac
    19141 acagcctgct gctaatcgct ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc
    19201 gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc gtagccaggg acgttgggta
    19261 atctggctct ctttcctcat tgcgctgttg ctgcaaatca tgccctggcc ggataacctg
    19321 attgttttcc ggccaaactg ggtgttactc atcttgttgt attggatcct ggccttgcct
    19381 catcgcgtaa atgtgggcac aggttttgtg atgggtgcca tactggatct gatcagcggc
    19441 tcgacgcttg gcgtacgcgt attggcgatg agcatcattg cttacctggt ggcgctgaaa
    19501 taccagcttt tccgcaacct cgcattatgg cagcaggcgc tggtcgtcat gttgctttcg
    19561 ctggtggtgg atattattgt tttctgggca gagtttttag tgattaacgt ctctttcaga
    19621 ccggaagtgt tctggagtag tgtagtcaat ggggtgctct ggccgtggat tttcttgctg
    19681 atgcgtaaag tccgtcagca gtttgcagtg caataaaggt ttctatgact tctctgtatt
    19741 tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg acctttgaac
    19801 gtattgttac gggcattgag gagcagcgtc agccgcagga gagcgcgcag cagtatgttg
    19861 tgcgtctggc gcgcgagaaa gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg
    19921 tgctgggtgc ggatactatc gttatcctga atggagaagt gctggagaaa ccgcgcgacg
    19981 cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg
    20041 cagtggcgtt ggccgacagc cagcacattc tcgattgcct ggtggtcacc gatgtgactt
    20101 tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc cagcggtgaa ccgttagata
    20161 aagcaggtgc atacggtatt caggggctgg gtggctgttt tgtcaggaag ataaatggca
    20221 gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta agtaatttta
    20281 acgcactgcg tgagaaaagg gataaacatg acggctgaat tgttagtaaa cgtaacgcct
    20341 tcggaaacgc gagtggcgta tattgatggc ggtattctgc aggaaattca tattgaacgt
    20401 gaggcgcgac gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg
    20461 ggtatgcagg cggcttttgt agatattggg ctggataaag ccgcgtttct tcatgcatct
    20521 gacatcatgc cgcacaccga atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc
    20581 gacatctcgg aactggttcg tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg
    20641 cttggcacta aaggtgcgcg cctgaccacc gatatcacgc tgccttctcg ctatctggtg
    20701 tttatgccag gggcttctca cgttggggtt tcccaacgta ttgaaagcga atcagaacgt
    20761 gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt tatcatccgt
    20821 accgcagcgg aaggggttgg cgaggctgaa ctggcctccg atgccgctta tctgaaacgc
    20881 gtctggacca aagttatgga gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc
    20941 gaactggcgc tggcgcagcg tgttctgcgt gatttcgccg acgccgaact ggaccgcatt
    21001 cgcgttgact cacgcctgac ttacgaagca ttgctggagt ttacctcgga gtacattccc
    21061 gagatgacca gcaagctgga gcattacacc ggacgccagc cgattttcga tctctttgat
    21121 gtcgaaaacg aaatccagcg agcgctggaa cgcaaagtag aactgaaatc cggtggttat
    21181 ctgattatcg accagaccga agcgatgacc accgtggaca tcaataccgg agcgtttgtc
    21241 ggtcatcgca atctggacga caccattttc aataccaata ttgaagcgac gcaggctatc
    21301 gctcgccagt tacggttgcg taatctgggt gggattatca ttattgattt catcgatatg
    21361 aataatgaag atcaccgccg ccgcgtgctg cactcgctgg agcaggcgtt gagcaaagac
    21421 cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc tggtggagat gacgcgtaaa
    21481 cgcacccgcg aaagcattga gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc
    21541 ggaacggtga aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc
    21601 caccatgctt acgactccga ccgtttcctg gtctatgcat ctccggcagt agctgaagcc
    21661 ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg caaacaggtt
    21721 aaagtacaaa ttgaaccgct ctataaccag gagcagtttg acgtcgtaat gatgtaaaca
    21781 gatgctgggc cgccatccgg caaagggttt ttgagtcaca tttttagcag acaaggagtg
    21841 acgggtgagg cgattgccgg ggattttact gcttactgga gccgcgctcg ttgtgatcgc
    21901 tgctctgctg gttagcggcc tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga
    21961 aatcctcaac aaaatagaat ccgcgactgg catgccggta gaagccagtc agctctcagc
    22021 cagctggcag aattttggcc cgacgcttga agcacacgac atccgtgcag aactaaaaga
    22081 tggcggcgaa ttttcggtta aacgcgttac tctggcgctg gatgtctggc agagcctgtt
    22141 acatatgcgc tggcagtttc gcgacctcac tttctggcag ctgcgctttc gcaccaacac
    22201 tcctatcacc agcggtggta gcgatgacag tctggaagcc agtcacatca gcgatctgtt
    22261 tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa gtcagttttc tgacgccatc
    22321 cggtcagcgc gccgagctgg cgatcccaca actcacctgg ctgaacgatc cacgtcgaca
    22381 ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga cagcacggcg tgatgcaggt
    22441 gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct ggctacaggc
    22501 ggatgacatc gacctgaagc cgtggctcgg taaatggatg caggacaata ttgctctgga
    22561 aacggcacag ttctcccttg aaggctggat gacgatcgac aaaggcgatg taaccggcgg
    22621 tgacgtctgg ctgaaacagg gcggtgccag ctggttgggc gagaagcaaa cgcatacgct
    22681 gtcggtggat aatctgaccg cgcatattac gcgtgaaaat ccgggctggc agttctctat
    22741 tcccgataca cggatcacga tggacggcaa accctggccg agcggagcat tgacgctggc
    22801 ctggataccg gaacaggacg ttggcggcaa agacaataaa cgcagtgacg aactccggat
    22861 tcgcgccagt aatctggagc tggcaggcct ggagggcata cgcccgctgg ccgcgaaact
    22921 ttcacctgca ctgggtgatg tttggcgctc cacacaaccg agcggcaaga ttaacactct
    22981 ggcgctggat atcccgcttc aggcggcaga caagacccgt tttcaggcat cgtggagcga
    23041 tctggcctgg aagcaatgga aattattacc gggtgcggaa cacttctccg ggacgctttc
    23101 cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga tgccttacga
    23161 aacggtattc cgtgcgccac tggaaatcgc cgacggccag gcaactataa gctggctgaa
    23221 caatgacaaa ggtttccagc tggatgggcg taatattgac gttaaagcca aagccgtcca
    23281 tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat gaaccctggc tgggtattct
    23341 ggctggcatc agtaccgatg atggttcaca agcctggcgc tatttcccgg aaaacttgat
    23401 gggtaaagac ctggttgatt acttaagtgg cgcgattcag ggcggtgaag cggataacgc
    23461 gacgctggtt tatggtggca atccgcaact cttcccctat aaacacaacg aaggtcagtt
    23521 tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc cagccggact ggcctgcatt
    23581 aactaacctt gatattgaac tggactttat taacgacggt ttatggatga aaaccgatgg
    23641 cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg actactcgaa
    23701 agaaaaactg ctgattgacg ctgacattaa aggtccgggt aaagccgttg gcccttactt
    23761 tgatgagaca ccgctgaaag attctctggg tgcgaccctg caagaactcc agctcgacgg
    23821 cgatgtgaat gctcgcttac atcttgatat cccgctgaac ggcgaactgg taaccgcgaa
    23881 aggtgaagtg acgctgcgta ataacagtct gtttatcaaa ccactcgaca gcaccctgaa
    23941 aaatttgagc ggtaaattca gctttatcaa tggcgatctg caaagtgaac cactgacagc
    24001 aagctggttt aatcagccgt tgaacgtgga tttttccacc aaagaagggg caaaagccta
    24061 ccaggttgcg gtgaatctca acggtaactg gcaaccggcg aaaaccagcg ttctgcctga
    24121 agcggtgaac gaagcattga gtggcagcgt ggcgtgggat ggtaaagtgg gcattgatct
    24181 gccttatcat gctggcgcga cctataacgt agagatgaac ggcgatctga ataatgtgag
    24241 tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa ccactagcgg taaacgttaa
    24301 ggttgatggc aatctcaaca gctttgaatt aaccggacag gctggtgcgg ataaccattt
    24361 caatagccgc tggttgcttg gtcaaaagct gacgctcgat cgtgctattt gggcggcaga
    24421 cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt gaactcaata tgccgccgat
    24481 gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct gcggagagtg tcggtggtgc
    24541 agcgagtttc ccacaacaca taacgttacg tacgcctatg ttgtcactgg gaaatcagca
    24601 atggaataac ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca
    24661 agggcgtgaa atcaacgcca cgctagcgat gcgtaataac gcgccgtggc tggcgaatat
    24721 caaatatctt tattacaacc cgagcgtggc gaaaactcgt ggtgattcaa cgccgtcatc
    24781 acctttcccg acaacggagc gcattaactt ccgtggctgg ccggacgcac aaatacgatg
    24841 cacagagtgc tggttctggg ggcaaaaatt cggtcgcatt gacagtgata tcaccatttc
    24901 tggcgatacg ttaacgctga ccaatggact gattgatact ggtttctcgc ggcttactgc
    24961 cgatggtgaa tgggttaata atccggagaa tgaacgtacc tcgctgaaag gaaaactgcg
    25021 cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc acgacgccca tacgccagtc
    25081 gtcatttaat gtggattacg atttacactg gcgcaaagca ccctggcaac cagatgaggc
    25141 gacgttgaat ggcatcattc atactcaact gggtaaaggc gaaattaccg aaatcaatac
    25201 cggacatgcc gggcaattgc tgcgcttatt gagcgttgat gccctgatgc gtaagctgcg
    25261 ttttgatttc agagacactt ttggcgaagg gttctatttt gactccattc gcagcaccgc
    25321 gtggattaaa gacggggtta tgcacaccga cgacacgctg gtggatggcc tggaggcgga
    25381 tatcgccatg aaagggtcgg taaatctggt acgtcgcgac ctgaatatgg aagcggttgt
    25441 cgcaccagag atttctgcga cggtgggcgt ggctgcggct tttgcggtta accccattgt
    25501 tggcgcggca gtatttgcag ccagtaaagt gctggggccg ctgtggagca aagtctccat
    25561 tttgcgctat cacatttcgg gtccgctgga cgatccgcaa atcaacgaag tgttgcgcca
    25621 accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg taattgcccc aatctcatat
    25681 gataatcgtt gccaaaggcc aacgagccgg aacataactg taggccggat agggcgttca
    25741 cgccgcatcc ggcagccgta aaaaatcctc tactgcagta actaacgagt agcaaaaacg
    25801 atgagtctga acctggtaag tgaacaattg ctagcggcga acggcctgaa acatcaggac
    25861 ttgttcgcga tcctcggtca actggccgaa cgtcgccttg attatggcga tctctatttt
    25921 cagtcgagct atcacgaatc ctgggtttta gaagaccgca ttattaaaga tggttcttac
    25981 aacatcgatc agggcgttgg tgtgcgtgca atcagtggtg aaaaaaccgg atttgcttac
    26041 gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc aagcggcgcg caccatcgtc
    26101 cgtgatagtg gtgatggcaa agtacagacg ctgggcgcgg tagagcatag cccgttgtat
    26161 acctcggtag atccgctgca aagcatgagc cgtgaagaga agctggatat cctgcgtcgc
    26221 gtcgataagg ttgcccgcga agcggacaag cgcgtacaag aagtgactgc cagcctcagc
    26281 ggcgtttatg aattaattct ggttgcggcc accgacggca cgctggcggc ggatgttcgt
    26341 ccgctggtgc gtctttccgt gagcgttctg gtcgaagaag atggcaaacg cgaacgcggt
    26401 gccagtggcg gcggcggtcg ttttggttat gagttcttcc ttgccgatct tgacggcgaa
    26461 gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc
    26521 gttgctgcac cagcgggcac catgccggta gtacttggcg caggttggcc gggcgtgctg
    26581 ttgcatgaag cggtaggtca cggtctggaa ggcgacttca accgccgcgg cacttcagta
    26641 tttagtgggc aggtcgggga gctggtggct tcagaactgt gtaccgtggt tgatgacggc
    26701 acgatggtcg atcgccgtgg ttcggtggcg attgatgacg aaggtacgcc aggccagtac
    26761 aacgtgctga ttgagaacgg cattctgaaa ggctacatgc aggataaact caacgcgcgt
    26821 ttgatgggga tgacgccgac tggcaacggt cgccgtgaat cctacgccca tctgcccatg
    26881 ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga ccccgcagga aattattgaa
    26941 tccgttgagt acggtatcta tgcaccgaac tttggtggcg gtcaggtgga tattacatcc
    27001 ggcaaattcg ttttctccac ttcagaagcg tatctgattg aaaacggtaa agtaacgaag
    27061 ccggtgaaag gcgcgacgtt gattggttcc ggtatcgaaa ctatgcagca gatttcgatg
    27121 gttggcaacg acctgaaact ggataacggc gtgggtgtct gcggtaaaga agggcaaagt
    27181 ttgccggttg gcgtgggcca gccaacgctg aaagttgata acctgactgt tggcggtact
    27241 gcgtaataat ttcattattt tcagaaggat aattaatctt tctacgtgca cgaacggtcc
    27301 cctcgctcct ctggggttag ggtgagggga acccgttggc acaggtttgt agaacgtaac
    27361 agtacaatat gaattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga
    27421 cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagcttat
    27481 ctttttcggt atataacgca taaaccgggc gtggatctga ctgataacgc ggcagcagga
    27541 tctccagctc accgcgattg atctcgttga tcacccacat cagcggcacg taggcgatcc
    27601 cggcacccgc cgttagccag cgcaccagcg tcatcggatc attagtcaca aatctccctt
    27661 gcgggatcag gcgagtcgag atcccttccg gtgcaatcag ttcaaattca ttgtcgggcc
    27721 gcacgctgta ttcaagccat gaatggctac ttaaatcggc gggtttttcc ggtatgccgt
    27781 attgtgtgag atagcttttc gcggcgcaca ccaccattgg catcgcgcca agacggcggg
    27841 aaaacaggct ggaatcctgc aacgcgccga cgcggatcac cacatccaga ccgtcggcaa
    27901 tcaggtcggg ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca
    27961 gcattttggc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
    28021 gtagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
    28081 catgaagcat acgacggcag ccctggtagt aaattctacc ggcttcggtc aggccaatgc
    28141 tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
    28201 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
    28261 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
    28321 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
    28381 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
    28441 gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag tctgtttccc
    28501 gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct tttatcactg
    28561 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
    28621 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
    28681 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
    28741 cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
    28801 cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc
    28861 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacagatact
    28921 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
    28981 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg
    29041 tgtgcaggcg atgtctcgcg aagagatcga ccaggctaac aacgtactac aaacggttct
    29101 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
    29161 ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt
    29221 tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc
    29281 ctatatggaa gaaactaagc tggaaggggt gcgtccgggg tatcgtgcag agatcacgcc
    29341 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgctg gggtcaccaa
    29401 cgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctaacc ttgaatgggt
    29461 gcgtcttgcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
    29521 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
    29581 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat cacgatgggt
    29641 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
    29701 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
    29761 gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
    29821 attcgttatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
    29881 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
    29941 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta cgcgtggggg
    30001 ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg
    30061 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg
    30121 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga gctggaaagt
    30181 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
    30241 gtcgataaag cctggggcga cctggtgcga cgcaccacgg cgctacaagg catgcgcagc
    30301 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
    30361 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
    30421 ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag
    30481 gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggaccgg ggaacgggaa
    30541 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
    30601 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
    30661 gaagtcaaag tagagtcagc cgaacgtcat catgcgatgg ttaacttctg gcgaaccaca
    30721 ctttcctgca ttctgggaac gcttttctgg ctgtggacgg gctggacttc cggcagtggt
    30781 gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
    30841 gtggcgatcg actttatcta cgggacgctg gccgcgctgc cgttagggct gctctacttt
    30901 ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag cctggcagtg
    30961 ctgggattct tcctcggaat agaagtacag aaacggcgac tgggctcgat gggggcgctg
    31021 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
    31081 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
    31141 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttctgcc
    31201 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
    31261 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
    31321 gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
    31381 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
    31441 agcgatgata aacgtcgtcg gtactttggt cagttgctgg aagaactgga aatctaccag
    31501 gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca tcggctggcg
    31561 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
    31621 agcgtcggtt ttttcatggc tatacttagc gatgaacggc agaactcgcc gcgaaacgtg
    31681 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
    31741 tttttatcgc gacttcagcc aagcctttgg tctggcgaaa gataaggtac gcgatctcga
    31801 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
    31861 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
    31921 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaaagcc
    31981 cccgaaccgg gggcaatatc gtcggacaag acgatgaggg tttatttgta cagttcagcc
    32041 gtagcgtgcc aggtgtcacc gctacgagct tcagtaatct ggtaggccgt tgcgcctttc
    32101 tcttccgctt ttttgttcag catttcacgt atatccattg gcgaagacgc cacaccactt
    32161 acggatacgg tcccgattgc ttcacggttt tgtgcttgtg cagcatcaat ggagtcggca
    32221 gcgaatgcac cgaaagagag aacagaaagt acgcttaatg cagcaacagt ggttttgatt
    32281 ttcatgattt ttacctcgac ataatctttt tgctgggtct ttgtttcgtg acccttatca
    32341 caaaatcaag tatacactaa ttattgaact aattaatacc acgctaactg tttctgttga
    32401 tactaagtgt taaaaatgtg aatgttaata acaaaaaaga attaaaaata cggttattta
    32461 tcgttaattc tagtgttttc aattagataa tgaattttgc ataatgtgct ttttttacct
    32521 tatgtattca ttgtgtgaat gacatgtcgc agtaaaacgc actattcgtt aaattaatcg
    32581 caggggaaag cagggggttg agagggataa gtaacatttt ccccgccgcc agaagcgacg
    32641 gggcagagat taaagctcct ggtcgaacag ctctaaaatc gcttcgtaca ggtctttgac
    32701 ggtgaaaccg ttagcagggg tggtaaagat ggtgtcatcg ccagcgatgg tgcccagaat
    32761 accttctgct ttgcccagtg agtccagcag gcgagcaatt aactgcgccg cgccagggct
    32821 ggtatgaatc acgacaactg catcgttgta gtcgatatcc agcaccagat ttttcaatgg
    32881 actggaggtg gttggtacac ccagttcagc tggcaggcag taaaccattt ccattttggc
    32941 attgcgtgta cgtacagcac caaacttggt caacatccgc gagactttag actgattaat
    33001 attgtcaaag ccttgctcct gcaacgcggc gacgatttcg ccctgggagc taaatttctc
    33061 ttctttaagt aatgctttaa atgctttaac tagttcttct tgcttagccg agcttcgcat
    33121 aagtcacccg atatggtggt tgatacaaca ttattgtgca tacagatgaa tttttatgca
    33181 aacagtcagc cctgaagaag gctgaaataa tgttatgaaa gagcgggatt ttatcaaatt
    33241 tcgttattga gaaacatgcc tgcgtcacgg catgcaaatt ctgcttaaaa gtaaattaat
    33301 tgttatcaaa ttgatgttgt tttggctgaa cggtagggta tattgtcacc acctgttgga
    33361 atgttgcgct aatgcataag cgactgttaa ttacgtaagt taggttcctg attacggcaa
    33421 ttaaatgcat aaacgctaaa cttgcgtgac tacacattct tgagatgtgg tcattgtaaa
    33481 cggcaatttt gtggattaag gtcgcggcag cggagcaaca tatcttagtt tatcaatata
    33541 ataaggagtt taggatgaaa gtcgcagtcc tcggcgctgc tggcggtatt ggccaggcgc
    33601 ttgcactact gttaaaaacc caactgcctt caggttcaga actctctctg tatgatatcg
    33661 ctccagtgac tcccggtgtg gctgtcgatc tgagccatat ccctactgct gtgaaaatca
    33721 aaggtttttc tggtgaagat gcgactccgg cgctggaagg cgcagatgtc gttcttatct
    33781 ctgcaggtgt agcgcgtaaa ccgggtatgg atcgttccga cctgtttaac gttaacgccg
    33841 gcatcgtgaa aaacctggta cagcaagttg cgaaaacctg cccgaaagcg tgcattggta
    33901 ttatcactaa cccggttaac accacagttg cgattgctgc tgaagtgctg aaaaaagccg
    33961 gtgtttatga caaaaacaaa ctgttcggcg ttaccacgct ggatatcatt cgttccaata
    34021 cttttgttgc ggaactgaaa ggcaaacagc caggcgaagt tgaagtgccg gttattggtg
    34081 gtcactctgg tgttaccatt ctgccgctgc tgtcacaggt tcctggcgtt agttttaccg
    34141 agcaggaagt ggctgatctg accaaacgta tccagaacgc gggtactgag gtggttgaag
    34201 cgaaagccgg tggcgggtct gcaaccctgt ctatgggcca ggcagctgca cgttttggtc
    34261 tgtctctggt tcgtgcactg cagggcgaac aaggcgttgt cgaatgtgcc tacgttgaag
    34321 gcgacggtca gtacgcccgt ttcttctctc aaccgctgct gctgggtaaa aacggcgtgg
    34381 aagagcgtaa atctatcggt accctgagcg catttgaaca gagcgcactg gaaggtatgc
    34441 tggatacgct gaagaaagat atcgccctgg gcgaagagtt cgttaataag taattaatta
    34501 gcgaataata aaaaaccgga gcacagactc cggttttttg ttttgagcac tcgacttaat
    34561 tggttgccgg atattcctga atggtgacct gcagcgttaa ctgcttatca tcacgcatca
    34621 ctacaaccgg gatcaccgaa ccagggcgaa tttctgccac ctgatccatc gtctccagag
    34681 cagagatggc cggtttgtta tccaccgaaa taatcagatc gttgacctga atacccgcat
    34741 tcgccgccgg gccgtcaggt gacacttcat taaccacgat cccttgcagt tgatctatac
    34801 caccgccctg cgcgtgcagt ggtgcgatct cgcgtccgcc gataccaatg tagccgcgga
    34861 tcacgcgacc atcgcggatc agcttatcca taattttggt tgctaactgg aaaggaatcg
    34921 caaagccgat accttccggc gtttcgccat cgttactctt atcaaacgac agcgtgttaa
    34981 tgcccatcag ttctcccagc gagttcacca gcgcgccgcc agagttaccg tggttaatgg
    35041 aagcatcggt ttgcaggaag ttttgccgcc cggtcgggtt cagaccgatt cgacccgtag
    35101 cactaataat cccctgggta atggtctgcc cgaggttgta cgggttaccg atcgccagta
    35161 ccacgtcgcc aatgtgcggt acgcgacgtg cattgatcgg aatggtaggt aaaccgccag
    35221 tggcattaat tttaagtacc gccagatcgg ttagagagtc agatcccacc agcaatgctt
    35281 caaatacacg tccatcctgt aaggcgacga tgatctgatc ggcgtcgttg atgacgtgtt
    35341 tattggtgat gatataaccg cgttgatcca tgattacacc ggatcccagg gtgcggatct
    35401 caagctggtt gtgagagttg gtgttcaaac cacggttgta aacgttaacc accgctggcg
    35461 cggcgcggcg aaccgccaga ttataactgg caggcgtctc atcggtactg tcaaattgcg
    35521 gagtggaaag cgggttaagg ctgcgcagcg aaggcatggc aaccagcaga atagcgccga
    35581 caattaatcc aatcgcaacg gaacgtaaga gcttcacaaa catgatggag gcgtcattaa
    35641 aaaagggaac ggcagcagca taccacgagt taaccggaca tcacacgtaa gcctgatgcc
    35701 cggtttacga cattaacgca tcagcagata gatgctttca ttaccgcgta caatttgcag
    35761 ggcgatgatg gccggttttg ccgccagcac tttacgcatt tcagcaatcg agttcacccg
    35821 atcgcggttg acgccaatga tcacatcgtc tttttgcaag ccagcctgag cagctgggct
    35881 tcctttgaca acttcatcga tcttaatacc tttgccgcca tcttttagct gaccatcgct
    35941 caacgttgca ccttccagcg ctggcgtgat catttcagcg ctggccgacg aagaggtgct
    36001 ggtatcaagc gtcacttcta cttccagtgg tttgccgtta cgcagcaggc caagcttcac
    36061 tttcgtgccc ggctctgtag tcgcgatacg agagcgcaac tcagcaaagc tattcagcgg
    36121 tttgccgttg aggctggtaa taatatcgcc cgctttgacg cccgcttttg ccgagccaga
    36181 acctggcaac acttcgctga caaacgcgcc acgctgcacg tcaaggttga aggctttggc
    36241 gatatcggca ctcatctcgg tgcctttgat gcctaacaaa ccgcgtttga tttcaccaaa
    36301 gtcgataagc tgctgcgcca gtgttcgcgc catattactg gggatggcaa atccaatccc
    36361 gacgctcccg ccgccaggcg caaggattgc agtgttgatg ccaattaact caccgttaag
    36421 gtttaatagt gcaccgccgg agttaccgcg gttaatggaa gcatctgtct ggataaagtt
    36481 ttccagacct tcaagattca acccgctgcg gcctaatgcg gaaacaatgc cagaggtggc
    36541 ggtttgccca aggccaaatg ggttaccgac cgctacggca aaatcaccga cgcgcaattt
    36601 atcggagtcg gcaatagcga tttgcgttaa tttgctcggg ttttgaattt gtaacagggc
    36661 gatatcgctc tggtcatcgc taccaatcag ttttgcatca aactcgcgcc catcattgag
    36721 ctgaatactg attttctgtg cctgattaat cacatggttg ttggtcagca catagccttt
    36781 actggcgttg atgatgacac cggaacctaa accttcgaag ggttgtgcag gttgatccgg
    36841 taaatcatca ccaaaaaact ttttgaattc ttccgggatt ttctgtccct gactggccgt
    36901 tccttccacc cgtacgctca ccactgccgg aagcactttt tccagcattg gagccagact
    36961 ggggagaggg gcctgatcgg caacctggcc tggaatcgac gcgacggcct gaaatgacgc
    37021 cgagagagtt aacccgacac ttaacgctaa tgcactcaac agctgggttt gttttttcat
    37081 tattcctgct ctcgtacctg aatgataaga aaagagattc aaaacagttt gatgttattg
    37141 aattttcaga cggctaacaa tgagacacca gattaaatga tagtcgggga gggagagaag
    37201 aggagggcgc aatggctgcg cccgaaaaat aaattagtcg cgcttcgcgc cagtacgcag
    37261 caggccggat gcgccttcag aatagtcgcg aggcatctgc accggtgcct gatcgttgct
    37321 ggcttcagac tctgccagac gattacggaa cgggtttgct tcagcagaca gttccggcag
    37381 caggctgctg gagcttttcg ccatgtgctg atacagctgg cgatagtcgt gcgccatggt
    37441 atccagtaat tccgcgctgc gggcaaagtg gctaaccagc tcttcgcgat actcgtccag
    37501 ttcagcttta ttcttttcca gttcgtactg caacgcctgt tgctggcgta gtttacgatt
    37561 accaaaacgc atggccacag caccaataat gatgccgacg actaacccaa ttagcgcata
    37621 ttcccaggtc atgaacatct cccgttgtct tttgtttccg tagggtgttg gcttcaggct
    37681 ccatgcctgc ggctgattat gccactataa ccgttaattc cacagaagtg gaatcccgac
    37741 tgcatatcgc gtagtgtaga acggcctttt tttcgtcaac cgtgaacaac ggcgcaccga
    37801 ttattcaagg aataacaata agatcatgca aagcgttacc ccaacatcgc aatacctgaa
    37861 ggagctcaat gaaggcagcc atcaacccga cgacgttcaa aaagaggccg tcagccgcct
    37921 ggaaattatt tatcaagaac tcatcaatag caggccacca gcccccagga cgagtgggct
    37981 aatggcgcgg gtcggtaagc tgtggggtaa acgcgaagac acaaagcata cgccagtgcg
    38041 tggcttatat atgtggggcg gtgtaggacg cgggaaaacc tggctgatgg accttttcta
    38101 tcaaagcctg ccgggagagc ggaaacagcg cctgcacttt caccgtttta tgctgcgggt
    38161 gcacgaagag ctaactgcct tacaggggca gaccgatccg ctggaaatta ttgccgatcg
    38221 ctttaaagcc gaaactgacg tgctctgttt tgacgaattt tttgtttctg atattaccga
    38281 cgccatgcta cttggcggtc tgatgaaagc cctgttcgcc cgcggcatta ccctggtagc
    38341 gacgtcaaat attccgccgg atgaacttta tcgaaatggc ctgcaacgtg cgcgttttct
    38401 gcctgcaatc gatgccatta aacagcattg tgatgtaatg aacgtggacg ctggtgttga
    38461 ttatcgactg cgtacactca ctcaggcgca tctgtggctt tcgccactca acgatgaaac
    38521 ccgggcgcag atggataaac tatggttggc gctggcgggg gcgaaacgag aaaattcacc
    38581 gacgttagaa atcaaccatc ggccattggc gacaatgggc gtcgagaacc agacgctggc
    38641 ggtctctttt actacgctgt gcgtcgacgc ccgcagtcag catgactata ttgcgctctc
    38701 acgtctcttt catacggtca tgttgtttga tgtaccagtt atgacgcggt tgatggagag
    38761 cgaagcgcgg cgctttattg cgctggtgga tgagttttac gagcgccatg tcaaattagt
    38821 ggtgagtgca gaagtgccgc tgtatgaaat ttatcagggc gatcggctga agtttgagtt
    38881 ccagcgttgc ctgtcacgtc tgcaagagat gcaaagcgaa gagtatctga agcgcgagca
    38941 tttggcgggt taaaacctgt cacaaatcac aaaaaggggt cgatctttga ccccgacttc
    39001 tctataatcc tgcgacccca cgttacaaga aagttttttt cccaaaactt tttgtgtgct
    39061 ggcataggct attcgaaggg gtaggtttgc cggactttgt cgtgtgaacc tcaacaattg
    39121 aagacgtttg ggtgttcacc aacgtgtaac tatttattgg gtaagctttt aatgaaaact
    39181 tttacagcta aaccagaaac cgtaaaacgc gactggtatg ttgttgacgc gaccggtaaa
    39241 actctgggcc gtctggctac tgaactggct cgtcgcctgc gcggtaagca caaagcggaa
    39301 tacactccgc acgtagatac cggtgattac atcatcgttc tgaacgctga caaagttgct
    39361 gtaaccggca acaagcgtac tgacaaagtg tactatcacc acaccggcca catcggtggt
    39421 atcaaacaag cgacctttga agagatgatt gctcgccgtc ctgagcgtgt gattgaaatc
    39481 gcggttaaag gcatgttgcc aaaaggcccg ctgggtcgtg ctatgttccg taaactgaaa
    39541 gtttacgcgg gtaacgagca caaccacgcg gcacagcaac cgcaagttct tgacatctaa
    39601 tcgggattat aggcaatggc tgaaaatcaa tactacggca ctggtcgccg caaaagttcc
    39661 gcagctcgcg ttttcatcaa accgggcaac ggtaaaatcg taatcaacca acgttctctg
    39721 gaacagtact tcggtcgtga aactgctcgc atggtagttc gtcagccgct ggaactggtc
    39781 gacatggttg agaaactgga cctgtacatc accgttaaag gtggtggtat ctctggtcag
    39841 gctggtgcga tccgtcacgg tatcacccgc gctctgatgg aatacgacga gtccctgcgt
    39901 tctgaactgc gtaaagctgg cttcgttact cgtgacgctc gtcaggttga acgtaagaaa
    39961 gtcggtctgc gtaaagcacg tcgtcgtccg cagttctcca aacgttaatt ggcttctgct
    40021 ccggcagaaa acaatttgcg aaaaaacccg cttcggcggg tttttttatg gttaaattct
    40081 gaatcagcgt aaaaattgga aagttgcttt ttgctaccgt ctgacagaca ggcaaaacaa
    40141 aaaccacatc gccaataagg gactaagtca actatttcag actaaagcgc atctcttttt
    40201 ccccatttcc ggcatcgact caccacaaag gtcgcaaaat ctggtaaact atcatccaat
    40261 tttctgccca aatgtcgggt attgctcatt ttttgtttga ttttcgaaca aagagagtcg
    40321 ttccttactg ggtaacacaa cttctgactg gccacctggt ggctggtagc agtaaaaatt
    40381 ctgactatac ctggaggttt tcatggctgt cgctgccaac aaacgttcgg taatgacgct
    40441 gttttccggt cctactgaca tctatagcca tcaggtccgc attgtgctgg ctgagaaagg
    40501 tgtaagtttc gagatcgaac acgtggaaaa ggacaatccg cctcaggatc tgattgacct
    40561 caacccgaat cagagcgttc cgaccctggt ggatcgtgag ctgaccctgt gggaatctcg
    40621 catcattatg gaatatctgg atgagcgttt cccgcatccg ccactgatgc ctgtttaccc
    40681 ggtagctcgc ggtgaaagcc gtctgtacat gcatcgcatc gaaaaagact ggtacacgct
    40741 gatgaacacc atcatcaacg gttcagcttc tgaagcagat gccgcacgta agcaactgcg
    40801 cgaagaactg ctggcgattg cgccggtctt cggtcagaag ccgtacttcc tgagcgatga
    40861 gttcagcctg gtcgattgct atcttgctcc gctgctgtgg cgtctgccgc aactgggcat
    40921 cgagttcagc ggcccgggtg cgaaagagct gaaaggctat atgacccgcg tctttgagcg
    40981 tgactctttc cttgcttctt taactgaagc agaacgtgaa atgcgtctgg gccggagtta
    41041 atctgtatgg atttgtcaca gctaacacca cgtcgtccct atctgctgcg tgcattctat
    41101 gagtggttgc tggataacca gctcacgccg cacttggtgg tggatgtgac gctccctggc
    41161 gtgcaggttc ctatggaata tgcgcgtgac gggcaaatcg tactcaacat tgcgccgcgt
    41221 gctgtcggca atctggaact ggcgaatgat gaggtacgct ttaacgcgcg ctttggcggc
    41281 attccgcgtc aggtttctgt gccgctggct gccgtgctgg ctatctacgc ccgtgaaaat
    41341 ggcgcaggca cgatgtttga gcctgaagct gcctacgatg aagataccag catcatgaat
    41401 gatgaagagg catcggcaga caacgaaacc gttatgtcgg ttattgatgg cgacaagcca
    41461 gatcacgatg atgacactca tcctgacgat gaacctccgc agccaccacg cggtggtcga
    41521 ccggcattac gcgttgtgaa gtaatacaaa aacaggccct ggtggcctgt tttgtctttt
    41581 aatggcttaa taaaatagcg tgattgtggc aatcatattc accacgaaac cgactgccat
    41641 ggggatcgct gttcgcttca ccaccgcaaa aggcgaaacg cctgcaattc ctgacgtaac
    41701 gaccaccacc gcagtaatcg gcgaaaccgc gcgcgctagc gtcgtggcaa aatgcatcgg
    41761 cataatcatt acaaccgctg gtacatgtag tccggctgcg atattcggaa taagactggc
    41821 aaatgacata aacggcgcat tgccagagcc catcacaatg gcacaaatgg caatgactag
    41881 ctccataata atcatcacgc caataccgcc cagaccagaa tgctcagcac ccctgataac
    41941 cgcatcaaca gtgccaatcg tcgttaagcc tttcgcaaat atttcacccg cgaccaccag
    42001 cgttaccacg ttggcaaact gcgtacccat gccgtcaaaa aacgcctgca catcgtccat
    42061 tgtttcgcgc aagttatgct tgcggaagaa ctcaacaaac atcgtcaccg tcaaactcag
    42121 taacatcacc accaccagat gcagttctga ctgcatcagc ccgatgtggg cgaggaacag
    42181 cgagccgagc atcaagatta acggcatcac aggtaaaatg gcgtaataga gcggcgggac
    42241 attatcgaga gcttttagct ctgcctgttc gtgattgata tttttatctt ttttgtcaaa
    42301 agcgcgttgc acgaaaaagt gggagatcgc caccgagata atgacgcaag aggcgaccgg
    42361 aagctggtag tggaagaagt aagtggcaat tttcattccc gcgacctggg ctgcaaaaat
    42421 ggagttcgtt tccagaatcc cccactcaat ggacatcgtg gttgcgataa ccgccaccgc
    42481 agagaggcga cttactccca gactcaccag cgtcggaaat aaggtgacca tcagcaacat
    42541 acccaggccg gaggcgctgg taataaactg cgccatgatt tggccgatga cgtaagttgc
    42601 cgacagaata atatacggcg agtgaatgag ttttaacggg cggcttagca agctcaccat
    42661 cgcgcgactg gctcctgtgc gctccatgta gcgggcataa ccgcccaccg ccataatcga
    42721 cagccccaat ccggcaatgc ggttggacag catgcgcagg atttcattat aaatatcgac
    42781 gatcaagtag ccgctgcttt tggcgggatc gagtacacca ccgaacccta accagacacc
    42841 gcacatcatc aggaagatac ctgcggcagc cagcaccacc tgaggtttgt agtttttcag
    42901 gatcaaatag cccatcgtaa ttaatacgat gacagatata attatgccga acattgttga
    42961 actccgtgtc aaaagaaaac ggtcaatccc ataaacggca gattgaaaac aatgatgtta
    43021 tattttttgc aagtctattt atggtgcgga tgtcgtgttt ttaattgtag gtgaggtaat
    43081 ttttcattaa aaaatatgca cttatgatta ttttgtaaga a
//
LOCUS       DAZFVO020000021        42919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFVO020000021 DAZFVO020000000
VERSION     DAZFVO020000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.021"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(123..632)
                     /gene="sulA"
                     /locus_tag="ACHKNB_RS05400"
                     /old_locus_tag="pgaptmp_001080"
     CDS             complement(123..632)
                     /gene="sulA"
                     /locus_tag="ACHKNB_RS05400"
                     /old_locus_tag="pgaptmp_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HGU4194639.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            851..1480
                     /gene="sxy"
                     /locus_tag="ACHKNB_RS05405"
                     /old_locus_tag="pgaptmp_001081"
     CDS             851..1480
                     /gene="sxy"
                     /locus_tag="ACHKNB_RS05405"
                     /old_locus_tag="pgaptmp_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HGU4194640.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1443..3605)
                     /gene="yccS"
                     /locus_tag="ACHKNB_RS05410"
                     /old_locus_tag="pgaptmp_001082"
     CDS             complement(1443..3605)
                     /gene="yccS"
                     /locus_tag="ACHKNB_RS05410"
                     /old_locus_tag="pgaptmp_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HGU4194641.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3615..4061)
                     /gene="yccF"
                     /locus_tag="ACHKNB_RS05415"
                     /old_locus_tag="pgaptmp_001083"
     CDS             complement(3615..4061)
                     /gene="yccF"
                     /locus_tag="ACHKNB_RS05415"
                     /old_locus_tag="pgaptmp_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HGU4194642.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4184..6238
                     /gene="helD"
                     /locus_tag="ACHKNB_RS05420"
                     /old_locus_tag="pgaptmp_001084"
     CDS             4184..6238
                     /gene="helD"
                     /locus_tag="ACHKNB_RS05420"
                     /old_locus_tag="pgaptmp_001084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HGU4194643.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6270..6728)
                     /gene="mgsA"
                     /locus_tag="ACHKNB_RS05425"
                     /old_locus_tag="pgaptmp_001085"
     CDS             complement(6270..6728)
                     /gene="mgsA"
                     /locus_tag="ACHKNB_RS05425"
                     /old_locus_tag="pgaptmp_001085"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HGU4194644.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6824..7486)
                     /gene="yccT"
                     /locus_tag="ACHKNB_RS05430"
                     /old_locus_tag="pgaptmp_001086"
     CDS             complement(6824..7486)
                     /gene="yccT"
                     /locus_tag="ACHKNB_RS05430"
                     /old_locus_tag="pgaptmp_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HGU4194645.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7659..8072
                     /gene="yccU"
                     /locus_tag="ACHKNB_RS05435"
                     /old_locus_tag="pgaptmp_001087"
     CDS             7659..8072
                     /gene="yccU"
                     /locus_tag="ACHKNB_RS05435"
                     /old_locus_tag="pgaptmp_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGU4194646.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(8117..8434)
                     /gene="hspQ"
                     /locus_tag="ACHKNB_RS05440"
                     /old_locus_tag="pgaptmp_001088"
     CDS             complement(8117..8434)
                     /gene="hspQ"
                     /locus_tag="ACHKNB_RS05440"
                     /old_locus_tag="pgaptmp_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HGU4194647.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8492..9682)
                     /gene="rlmI"
                     /locus_tag="ACHKNB_RS05445"
                     /old_locus_tag="pgaptmp_001089"
     CDS             complement(8492..9682)
                     /gene="rlmI"
                     /locus_tag="ACHKNB_RS05445"
                     /old_locus_tag="pgaptmp_001089"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HGU4194648.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9777..10055
                     /gene="yccX"
                     /locus_tag="ACHKNB_RS05450"
                     /old_locus_tag="pgaptmp_001090"
     CDS             9777..10055
                     /gene="yccX"
                     /locus_tag="ACHKNB_RS05450"
                     /old_locus_tag="pgaptmp_001090"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGU4194649.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(10052..10381)
                     /gene="tusE"
                     /locus_tag="ACHKNB_RS05455"
                     /old_locus_tag="pgaptmp_001091"
     CDS             complement(10052..10381)
                     /gene="tusE"
                     /locus_tag="ACHKNB_RS05455"
                     /old_locus_tag="pgaptmp_001091"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HGU4194650.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10472..11131)
                     /gene="yccA"
                     /locus_tag="ACHKNB_RS05460"
                     /old_locus_tag="pgaptmp_001092"
     CDS             complement(10472..11131)
                     /gene="yccA"
                     /locus_tag="ACHKNB_RS05460"
                     /old_locus_tag="pgaptmp_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HGU4194651.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11338..11425)
                     /locus_tag="ACHKNB_RS05465"
                     /old_locus_tag="pgaptmp_001093"
     tRNA            complement(11338..11425)
                     /locus_tag="ACHKNB_RS05465"
                     /old_locus_tag="pgaptmp_001093"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11389..11391),aa:Ser,seq:tga)
     gene            11852..12970
                     /gene="hyaA"
                     /locus_tag="ACHKNB_RS05470"
                     /old_locus_tag="pgaptmp_001094"
     CDS             11852..12970
                     /gene="hyaA"
                     /locus_tag="ACHKNB_RS05470"
                     /old_locus_tag="pgaptmp_001094"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HGU4194652.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12967..14760
                     /gene="hyaB"
                     /locus_tag="ACHKNB_RS05475"
                     /old_locus_tag="pgaptmp_001095"
     CDS             12967..14760
                     /gene="hyaB"
                     /locus_tag="ACHKNB_RS05475"
                     /old_locus_tag="pgaptmp_001095"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HGU4194653.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14779..15486
                     /gene="hyaC"
                     /locus_tag="ACHKNB_RS05480"
                     /old_locus_tag="pgaptmp_001096"
     CDS             14779..15486
                     /gene="hyaC"
                     /locus_tag="ACHKNB_RS05480"
                     /old_locus_tag="pgaptmp_001096"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HGU4194654.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15483..16070
                     /gene="hyaD"
                     /locus_tag="ACHKNB_RS05485"
                     /old_locus_tag="pgaptmp_001097"
     CDS             15483..16070
                     /gene="hyaD"
                     /locus_tag="ACHKNB_RS05485"
                     /old_locus_tag="pgaptmp_001097"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HGU4194655.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            16067..16465
                     /gene="hyaE"
                     /locus_tag="ACHKNB_RS05490"
                     /old_locus_tag="pgaptmp_001098"
     CDS             16067..16465
                     /gene="hyaE"
                     /locus_tag="ACHKNB_RS05490"
                     /old_locus_tag="pgaptmp_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HGU4194656.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16462..17319
                     /gene="hyaF"
                     /locus_tag="ACHKNB_RS05495"
                     /old_locus_tag="pgaptmp_001099"
     CDS             16462..17319
                     /gene="hyaF"
                     /locus_tag="ACHKNB_RS05495"
                     /old_locus_tag="pgaptmp_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HGU4194657.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17453..18997
                     /gene="appC"
                     /locus_tag="ACHKNB_RS05500"
                     /old_locus_tag="pgaptmp_001100"
     CDS             17453..18997
                     /gene="appC"
                     /locus_tag="ACHKNB_RS05500"
                     /old_locus_tag="pgaptmp_001100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HGU4194658.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            19009..20145
                     /gene="appB"
                     /locus_tag="ACHKNB_RS05505"
                     /old_locus_tag="pgaptmp_001101"
     CDS             19009..20145
                     /gene="appB"
                     /locus_tag="ACHKNB_RS05505"
                     /old_locus_tag="pgaptmp_001101"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HGU4194659.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            20158..20250
                     /gene="cbdX"
                     /locus_tag="ACHKNB_RS05510"
                     /old_locus_tag="pgaptmp_001102"
     CDS             20158..20250
                     /gene="cbdX"
                     /locus_tag="ACHKNB_RS05510"
                     /old_locus_tag="pgaptmp_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HGU4194660.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20330..21628
                     /gene="appA"
                     /locus_tag="ACHKNB_RS05515"
                     /old_locus_tag="pgaptmp_001103"
     CDS             20330..21628
                     /gene="appA"
                     /locus_tag="ACHKNB_RS05515"
                     /old_locus_tag="pgaptmp_001103"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HGU4194661.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSRQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21743..23923)
                     /gene="etk"
                     /locus_tag="ACHKNB_RS05520"
                     /old_locus_tag="pgaptmp_001104"
     CDS             complement(21743..23923)
                     /gene="etk"
                     /locus_tag="ACHKNB_RS05520"
                     /old_locus_tag="pgaptmp_001104"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGU4194662.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23943..24389)
                     /gene="etp"
                     /locus_tag="ACHKNB_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /gene_synonym="yccY"
     CDS             complement(23943..24389)
                     /gene="etp"
                     /locus_tag="ACHKNB_RS05525"
                     /old_locus_tag="pgaptmp_001105"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HGU4194663.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24377..25516)
                     /gene="gfcE"
                     /locus_tag="ACHKNB_RS05530"
                     /old_locus_tag="pgaptmp_001106"
     CDS             complement(24377..25516)
                     /gene="gfcE"
                     /locus_tag="ACHKNB_RS05530"
                     /old_locus_tag="pgaptmp_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HGU4194664.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25562..27658)
                     /gene="gfcD"
                     /locus_tag="ACHKNB_RS05535"
                     /old_locus_tag="pgaptmp_001107"
     CDS             complement(25562..27658)
                     /gene="gfcD"
                     /locus_tag="ACHKNB_RS05535"
                     /old_locus_tag="pgaptmp_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4194665.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27658..28404)
                     /gene="gfcC"
                     /locus_tag="ACHKNB_RS05540"
                     /old_locus_tag="pgaptmp_001108"
     CDS             complement(27658..28404)
                     /gene="gfcC"
                     /locus_tag="ACHKNB_RS05540"
                     /old_locus_tag="pgaptmp_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4194666.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28401..29045)
                     /gene="gfcB"
                     /locus_tag="ACHKNB_RS05545"
                     /old_locus_tag="pgaptmp_001109"
     CDS             complement(28401..29045)
                     /gene="gfcB"
                     /locus_tag="ACHKNB_RS05545"
                     /old_locus_tag="pgaptmp_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HGU4194667.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(29152..29457)
                     /gene="gfcA"
                     /locus_tag="ACHKNB_RS05550"
                     /old_locus_tag="pgaptmp_001110"
     CDS             complement(29152..29457)
                     /gene="gfcA"
                     /locus_tag="ACHKNB_RS05550"
                     /old_locus_tag="pgaptmp_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HGU4194668.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(29899..30111)
                     /gene="cspH"
                     /locus_tag="ACHKNB_RS05555"
                     /old_locus_tag="pgaptmp_001111"
     CDS             complement(29899..30111)
                     /gene="cspH"
                     /locus_tag="ACHKNB_RS05555"
                     /old_locus_tag="pgaptmp_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HGU4194669.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            30397..30609
                     /gene="cspG"
                     /locus_tag="ACHKNB_RS05560"
                     /old_locus_tag="pgaptmp_001112"
     CDS             30397..30609
                     /gene="cspG"
                     /locus_tag="ACHKNB_RS05560"
                     /old_locus_tag="pgaptmp_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HGU4194670.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            30620..30808
                     /gene="ymcF"
                     /locus_tag="ACHKNB_RS05565"
                     /old_locus_tag="pgaptmp_001113"
     CDS             30620..30808
                     /gene="ymcF"
                     /locus_tag="ACHKNB_RS05565"
                     /old_locus_tag="pgaptmp_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4194671.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            30783..31013
                     /gene="ymcE"
                     /locus_tag="ACHKNB_RS05570"
                     /old_locus_tag="pgaptmp_001114"
     CDS             30783..31013
                     /gene="ymcE"
                     /locus_tag="ACHKNB_RS05570"
                     /old_locus_tag="pgaptmp_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HGU4194672.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            31003..31200
                     /locus_tag="ACHKNB_RS05575"
                     /old_locus_tag="pgaptmp_001115"
     CDS             31003..31200
                     /locus_tag="ACHKNB_RS05575"
                     /old_locus_tag="pgaptmp_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4194673.1"
                     /translation="MNIEELKKQAETEIADFIAQKIADFIAQKIAELNKNTGKEVSEI
                     RFTAREKMTGLESYDVKIKIM"
     gene            complement(31248..32321)
                     /gene="yccM"
                     /locus_tag="ACHKNB_RS05580"
                     /old_locus_tag="pgaptmp_001116"
     CDS             complement(31248..32321)
                     /gene="yccM"
                     /locus_tag="ACHKNB_RS05580"
                     /old_locus_tag="pgaptmp_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4194674.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(32393..35137)
                     /gene="torS"
                     /locus_tag="ACHKNB_RS05585"
                     /old_locus_tag="pgaptmp_001117"
     CDS             complement(32393..35137)
                     /gene="torS"
                     /locus_tag="ACHKNB_RS05585"
                     /old_locus_tag="pgaptmp_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HGU4194675.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYF
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            35220..36248
                     /gene="torT"
                     /locus_tag="ACHKNB_RS05590"
                     /old_locus_tag="pgaptmp_001118"
     CDS             35220..36248
                     /gene="torT"
                     /locus_tag="ACHKNB_RS05590"
                     /old_locus_tag="pgaptmp_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HGU4194676.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(36221..36913)
                     /gene="torR"
                     /locus_tag="ACHKNB_RS05595"
                     /old_locus_tag="pgaptmp_001119"
     CDS             complement(36221..36913)
                     /gene="torR"
                     /locus_tag="ACHKNB_RS05595"
                     /old_locus_tag="pgaptmp_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HGU4194677.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            37043..38215
                     /gene="torC"
                     /locus_tag="ACHKNB_RS05600"
                     /old_locus_tag="pgaptmp_001120"
     CDS             37043..38215
                     /gene="torC"
                     /locus_tag="ACHKNB_RS05600"
                     /old_locus_tag="pgaptmp_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HGU4194678.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            38215..40761
                     /gene="torA"
                     /locus_tag="ACHKNB_RS05605"
                     /old_locus_tag="pgaptmp_001121"
     CDS             38215..40761
                     /gene="torA"
                     /locus_tag="ACHKNB_RS05605"
                     /old_locus_tag="pgaptmp_001121"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HGU4194679.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            40758..41357
                     /gene="torD"
                     /locus_tag="ACHKNB_RS05610"
                     /old_locus_tag="pgaptmp_001122"
     CDS             40758..41357
                     /gene="torD"
                     /locus_tag="ACHKNB_RS05610"
                     /old_locus_tag="pgaptmp_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HGU4194680.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(41450..41755)
                     /gene="cbpM"
                     /locus_tag="ACHKNB_RS05615"
                     /old_locus_tag="pgaptmp_001123"
     CDS             complement(41450..41755)
                     /gene="cbpM"
                     /locus_tag="ACHKNB_RS05615"
                     /old_locus_tag="pgaptmp_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HGU4194681.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(41755..42675)
                     /gene="cbpA"
                     /locus_tag="ACHKNB_RS05620"
                     /old_locus_tag="pgaptmp_001124"
     CDS             complement(41755..42675)
                     /gene="cbpA"
                     /locus_tag="ACHKNB_RS05620"
                     /old_locus_tag="pgaptmp_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HGU4194682.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        10434 a        10891 c        11259 g        10335 t
ORIGIN      
        1 cgacaattta cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt
       61 agtcacgact gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt
      121 acttaatgat acaaattaga gtgaattttt agcccggaaa gttgtctcgt ggcgtgagag
      181 gatgcgctta ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca
      241 agttcagcat gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa
      301 ttgcccgtgc gtaaagcgcg aaccattgac tccacagtgt ggcaagggga gagctggcta
      361 atctgcatta ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt
      421 tgttgcggtg ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc
      481 aacagtagaa gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc
      541 ccggctgtag tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac
      601 gaagaacgat gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt
      661 atggatgtac agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg
      721 caaattttgt cgttggtgac gggaaaacat aaattaatct tgccccttaa gaataagttg
      781 cctattttcg tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa
      841 aaggaatttt atgaaaagcc tctcctataa gcggatctat aaatcacaag aatacctggc
      901 aacgttgggc acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgacga
      961 cacggtgttt gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc
     1021 acagtactgt gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt
     1081 taccctcaat tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg
     1141 tctgtcgaaa tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga
     1201 aagactgaaa gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg
     1261 gattaaggat gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg
     1321 gcagcaaaac agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg
     1381 cattcatgaa gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc
     1441 tcttacgccg aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca
     1501 atctcaggca gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg
     1561 tctgcccgtg gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg
     1621 cgttcttcat cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcctcatca
     1681 agaaacgcca gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata
     1741 tagctggtaa acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg
     1801 gtaacgttcg gttcgctgga catatttgat accaccgacg ccagttcagc atcacggttg
     1861 tgtgcatcgc ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc
     1921 tccagtatgg catcgagata ccggcagttg gcctctgtgg cgcgttcgag cattcgcggc
     1981 agattgcgaa actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa
     2041 ccaatcagcg tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagcaagtta
     2101 aaacacagta gcaccaaaag tgtgatgaac atcgttgcat gggcatattg cacgttacgg
     2161 aaggcaaaaa agagcacgcc ggtaataacc agtagcacca gctgcccttc cagtgacggt
     2221 acaaaccaca gcacaggaat gccaatggcg ataccgacca gcgtaccaat aatccttaac
     2281 ttcaggcggt ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag
     2341 atccaatacc cgtgatgcat tccggttatt tgaatgatgg cgtagccgaa gcacaacacc
     2401 agcgacattc ttatcgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga
     2461 cgcagccaga tatcactcaa cccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta
     2521 ttatggggta gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg
     2581 cgtaaattgt tcagcaaaaa tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg
     2641 cgcatccgct ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt
     2701 tgataaggct gacgcaacaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc
     2761 gacatcagcc gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt
     2821 tgatactgaa tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa
     2881 tgcagcgtgc gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag
     2941 agtttcgtct gattcaatgt cgccatcagc agaccgttgg cgagagccaa atcgtacagc
     3001 ggtgcctggc tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa
     3061 cgcgccagtt gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc
     3121 agatgaccaa taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc
     3181 tgctgatacc agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat
     3241 gcaccgaagg caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg
     3301 gtagagagcg ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag
     3361 gccgaggcga taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt
     3421 cggtcatcga gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt
     3481 tttacatcac ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc
     3541 gcgtaataca gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag
     3601 gccatattag tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct
     3661 gtttctaccg ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag
     3721 ttcgcaatgc cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg
     3781 tgcatcaggc ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg
     3841 ccagtattca gcagcacatt tttgccagcc gggttcagtt catcgacatg aatagcttca
     3901 ttgccataag gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag
     3961 gtaaaaatca gcacaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat
     4021 ccgccaagca caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg
     4081 gttttcagta atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac
     4141 ggttacactg ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac
     4201 aacgcttgga aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg
     4261 gattaaagtc tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat
     4321 tcactgtaag cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt
     4381 ggtgcgtctg cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc
     4441 tcactggcgg cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca
     4501 actggatttg attgccacgc gcactggaga aaataaatgg ctgacgcgtg agcaaacctc
     4561 tggcgttcag caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact
     4621 ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat
     4681 tgaaagcgct cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc
     4741 ggattttttc cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt
     4801 taatggcgag cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct
     4861 ggtggcccgt gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt
     4921 gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag cgtattcgcg aacggctaca
     4981 taccgaagac attaccgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg
     5041 cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact
     5101 ctttattgct gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg
     5161 gcaatggctg acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga
     5221 aaaattacag cgtcgtcttg cctcacgcct cgatcgttgg gtaagtctga tgcggatgca
     5281 cggtggtgca caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag
     5341 taaacgtatc aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga
     5401 aaacgctgtc gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggccg
     5461 ctttatcagc ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg
     5521 cgcagcgttg ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg
     5581 tgatgactgg caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt
     5641 ccatgaaaac tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag
     5701 tcgtatcggt gaggtggcaa accggtttat tcagcagaac ccaggccagc tgaaaaagcc
     5761 gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg ttattggatg agagtcaact
     5821 tgacgctttg ctggataagc tctctggtta tgccaaaccg gaagagcgca ttctgatcct
     5881 ggcgcgttac catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa
     5941 gttgcaaatc gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat
     6001 catcgttggc ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat
     6061 ggaagaggcg ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat
     6121 gtacgtggcg ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc
     6181 ctctcccttt gtggaaatac tgaaaaatct ggatgtgccg gtagcgagaa aaccgtaaga
     6241 aacaggtggc gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga
     6301 taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat tatgaagtct
     6361 gccgttgcca cgttggtggc gaccggaatg ttccataccg tcgccagacg caacaaggct
     6421 ttcacgtcag gatcgtgcgg cacggcattt agtgggtccc agaagaaaat caatacatca
     6481 attttccctt ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac
     6541 atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt taccggtagt gcctgttgca
     6601 tacagtacgt gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt
     6661 ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc
     6721 agttccataa tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct
     6781 gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt
     6841 tttccgccca ttgtaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact
     6901 tcagccgttg ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac
     6961 ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt
     7021 tcgcggattt gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg
     7081 taatggcgag aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa
     7141 ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa
     7201 aattgacctg gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca
     7261 gccgttcttc actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg
     7321 gtccattatc cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc
     7381 cgtcgagtac cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata
     7441 ccggcaaaca taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc
     7501 aaaagaccgc ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat
     7561 agacatattt cccgcttttg ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg
     7621 ctttgtcgta cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag
     7681 gcattttaac gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc
     7741 ccagctatcg cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgc
     7801 caaaagttgc cggcaaaacg ctgctgggcc agcaggggta tggcacgctg gcagacgttc
     7861 cggaaaaggt cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac
     7921 aggaagctat tgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc
     7981 aagcggcagt actggcacgg gatgccgggt tgaatgtggt gatggatcgc tgcccggcta
     8041 ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg
     8101 gggattcgtt cagggatcag ttacgcagac gcggggcctg gagttgtttg cggatggtct
     8161 gcgccagctc atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct
     8221 gggcttcagc caggtaggta tgaaccggta ggccgttatc gtcctccatc actacgtgat
     8281 accacggagc ggcgcgaagc tcgtcattta ccgccagctc atcaggcgac ggttcagaga
     8341 gcgaataaac cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga
     8401 cctgctggcc gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct
     8461 ttgatataag ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca
     8521 gatatagccc ttccggatag gtagcgatca ccggatgatc ggctgcctga cggaactgct
     8581 ctataaattg tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata
     8641 aatcgctggt catcagaccg gaacaggaga aagtcaggag aataccgcct tcattcagca
     8701 actgaatcgc cagcatgttg atatctttat agccacgaca cgcgcccatc aactggcttt
     8761 tattctcaac aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc
     8821 gataagtacg cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca
     8881 gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc
     8941 tgacaacctg gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga
     9001 aacagttcag cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctatcacgct
     9061 ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc caccagcagt ttcattccgt
     9121 gttcttcaat cggcagcagg gcaggtggca actcgccggt gacggggccc tgggtcagct
     9181 ccattccttc ttttttacgt accgcgacgt cgctgcgatc gtaaatcgaa cattccgggt
     9241 acagcgtttg cagggcacta attaatgccg cgcgctgata ttctgcccca gcactcagca
     9301 gttgcagcac cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt
     9361 ctccggcgat taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt
     9421 tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa
     9481 acgtccagac gcgcgcccgg atttgcgaag ctggcgaata agcgccgcgt gctaaccatt
     9541 ttccctgatg atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg
     9601 caacggcccc ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg
     9661 ctaacactaa acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat
     9721 tgtccggagt tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt
     9781 cgaaagtctg cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca
     9841 ccacacagta cgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg
     9901 gcagcgtgga agtggttgct tgcggtgaag aagggcaggt ggaaaaatta atgcagtggt
     9961 taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc
    10021 cctcggggga attaaccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc
    10081 cagcaatttt ggtggcttgc ttagccggac ctttcggaaa cagtcggtat aaatagcggc
    10141 tgttaccttt ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg
    10201 gagaagtatt gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttccg
    10261 gtgacagcga aatcccttcg ttctctgcaa tcaccacagc cagcggctca ctccactggc
    10321 tgctttcttt gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca
    10381 taattattac taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa
    10441 agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg gctagcgaag cccagaatgc
    10501 tcagcaggct gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat
    10561 agttcgtttc accgccatga atgatgttgc tggtttcaaa caaaatagcg ccagaggaga
    10621 tcaaaatgaa gaccgcgctg atcgccagat gcagagcagg cagctgcagg aagatattcg
    10681 caaccatacc aatcagcacc accacaatac ccgccatcag cataccaccg aggaacgaca
    10741 tatctttgcg ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc
    10801 ccagtgccat agcgattacg tcacccattc cggcagacag ataggtgttc agaataggtc
    10861 cgaggatata acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat
    10921 tcgccgtttt ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac
    10981 ccggagatgg cagcatcagc accgtactgg cagttgcggt aatcgccgaa aaggccagcg
    11041 tcaggctcag cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac
    11101 ggtcatgtga agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta
    11161 attaagcagc ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat
    11221 ttgatctgga acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga
    11281 gaatttcgac aggcaaaaga aaaaggggtt agcatttagc taacccccta tcttatttgg
    11341 cggaagcgca gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccggtg
    11401 ccttcaaccg ctcggccaca cttccggaat gacgcgcact ataaacatcc cgatgcggcg
    11461 tgtaaacccc taatttgttt gtttgcctga aaaacagcca aaagtgcatt gatagcgtga
    11521 aataacagca gattgatcat ttcatcacca tgaattcctt ctcttttact cgtttagcaa
    11581 ccggctaaac atccccaccg cccggccaaa agaaaaatag gtccattttt atcgctaaaa
    11641 gataaatcca cacagtttgt attgttttgt gcaaaagttt cactacgctt tattaacaat
    11701 actttctggc gacgtgtgcc agtgcagaag gatgagcttt cgttttcagc atctcacgtg
    11761 aagcgatggt ttgccttgct acagggacgt cgcttgccga ccataagcgc ccggtgtcct
    11821 gccggtgtcg caaggagaag agacgtgcga tatgaataac gaggaaacat tttaccaggc
    11881 catgcggcgt cagggcgtta cccggcgcag ctttctcaaa tattgtagtc tggctgccac
    11941 gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc tgggcgctgg agaacaaacc
    12001 ccgcattccg gtggtatgga tccacggtct ggaatgcacc tgctgtaccg aatcttttat
    12061 ccgctccgct cacccactgg cgaaggacgt catcctttcc ctgatttccc tcgattatga
    12121 cgatactttg atggctgccg ccggaaccca ggcggaagaa gtcttcgaag acatcatcac
    12181 gcaatacaat ggcaaatata tcctcgcagt agaaggtaat ccgccgctgg gcgagcaggg
    12241 gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa ctcaaacgtg ccgctgccgg
    12301 agccagcgcg attatcgcct ggggaacctg cgcgtcctgg ggctgcgtgc aggccgcgcg
    12361 gcccaatccg acgcaggcaa cgcctatcga caaagtcatc accgacaaac ccattatcaa
    12421 agtacctggc tgcccgccga tcccggatgt gatgagcgcc atcattactt acatggtgac
    12481 ctttgatcgc ttgccagatg tcgacagaat gggccgtccg ctgatgttct atggtcagcg
    12541 aatccacgat aaatgctatc gccgcgccca cttcgacgcc ggagagttcg tccagagttg
    12601 ggatgatgac gctgcccgca aaggttactg cctgtacaaa atgggctgca aagggcctac
    12661 cacctataac gcctgttcct ccacacgctg gaatgatggc gtttctttcc caatccagtc
    12721 tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg gatcgcggtt cgttctacag
    12781 ccgcgtggtc gatattccgc aaatgggtac tcattccacc gccgataccg tcggtttaac
    12841 cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca gtcgccagcg ccgttgacca
    12901 gcgcagacgt cataaccagc aacctacaga aaccgaacat cagccaggca atgaggataa
    12961 acaggcatga gcactcagta cgaaactcag ggatacacca tcaataatgc cggacgccgc
    13021 ctggtggtcg acccgattac gcgcatcgaa ggccacatgc gctgcgaagt gaatattaac
    13081 gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca tgtttcgcgg gctggagatc
    13141 atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg ttgaacgtat ctgcggcgtc
    13201 tgtactggcg tacacgccct ggcttcggtt tacgccatcg aagatgctat cggtattaaa
    13261 gtgccggaca acgccaatat catccgcaac attatgctgg caacgctctg gtgccacgat
    13321 catctggtgc acttctatca gcttgccggg atggactgga tcgatgtgtt agatgcgctg
    13381 aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc tctcctcttg gccgaaatca
    13441 tcccctggct atttcttcga cgtacaaaac cgcctgaaaa aatttgttga aggcgggcag
    13501 ttggggatct tccgcaatgg ctactggggg cacccgcagt acaaactgcc gccagaagct
    13561 aacctgatgg gctttgccca ctatctcgaa gctctcgatt tccagcgtga aattgtcaaa
    13621 atccacgcgg tctttggcgg taaaaacccg catccaaact ggattgtcgg cgggatgcct
    13681 tgcgccatca acattgacga aagcggcgcg gtcggggcag tcaatatgga acgcctgaac
    13741 ctggtgcagt caattatcac ccgcacggcg gacttcatta acaacgtgat gatccccgac
    13801 gccttagcca tcggtcagtt caacaaaccg tggagcgaaa tcggcactgg tctttctgat
    13861 aaatgcgttc tcagctacgg cgcattcccg gatattgcca acgactttgg cgagaaaagt
    13921 ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca acaatgtgct gccagtggat
    13981 ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg cctggtatcg atatcccaac
    14041 gatcaggtcg ggcgtcatcc gttcgatggc atcaccgacc cgtggtacaa ccccggcgat
    14101 gtcaaaggca gcgataccaa cattcagcag ctgaatgaac aggaacgcta ctcgtggatc
    14161 aaagcgccac gctggcgcgg taacgcgatg gaagtggggc cgctggcgcg cacgttaatc
    14221 gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg atcgcatgat gtcggcgttg
    14281 aacctgccgc tttccggtat ccagtcaacg ttaggccgca ttttgtgccg cgcgcacgaa
    14341 gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca agctgatgac caacctgaaa
    14401 aacggcaatc tcgccactgc ttccacggaa aaatgggaac ctgcaacctg gccgacagag
    14461 tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt taggccactg ggccgccatt
    14521 cgcgatggca agattgatct ctaccagtgc gtggtgccga ccacctggaa cgccagcccg
    14581 cgcgatccca aagggcagat tggcgcttat gaagcggcgc tgatgaacac caaaatggcg
    14641 atccccgagc aaccgctgga gatcctgcgt actctgcaca gctttgaccc gtgcctcgcc
    14701 tgttcaacac acgtgctggg cgacgacggt agcgagctga tctccgtgca ggtgcgttaa
    14761 cagcgaagga gaatcatcat gcaacagaaa agcgacaacg ttgtcagcca ctatgtcttt
    14821 gaagcgccag tccgcatctg gcactggttg acggtgttat gcatggcggt gttgatggtc
    14881 accggatact ttatcggcaa gccgctacct tccgtcagcg gcgaggcgac gtatctgttc
    14941 tatatgggct acatcaggtt aattcacttc agcgccggga tggtttttac cgtggttttg
    15001 ctgatgcgga tctactgggc ttttgttggc aatcgatact cccgcgagct gtttatcgtg
    15061 ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg aaatccgctg gtatctgttt
    15121 ctggcaaaac gtccgagtgc cgatataggc cataatccca tcgcccaggc ggcgatgttc
    15181 ggctatttcc tgatgtcggt ctttatgatc atcactggtt ttgcgctgta cagcgaacac
    15241 agccagtacg ctatttttgc gccgttccgt tatgtggtgg aatttttcta ctggacgggt
    15301 ggcaactcaa tggacattca cagctggcat cggctgggga tgtggctgat tggcgcgttt
    15361 gtgatcggtc atgtctacat ggcgctgcgt gaagacatca tgtccgacga cacggtgatc
    15421 tccaccatgg tcaacggcta ccgtagccac aaatttggca aaataagtaa caaggagcgt
    15481 tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa cctgctgtgg gccgatgaag
    15541 gcttcggcgt gcgggtggcg gaacggctgt atgcccatta ccactggccc gagtatgtgg
    15601 agattgtcga tggcggtact cagggactga acttgctggg gtatgtcgaa agcgccagcc
    15661 atctgttgat tctcgatgcc attgactacg ggctggaacc tggaacgctg cgaacctatg
    15721 ccggagaacg cattccggct tatctcagcg cgaagaaaat gagcctgcat cagaacagtt
    15781 tctccgaagt gctggcgctg gcagatatcc gcggacatct gccagcacat attgccctcg
    15841 tcggtctgca acccgctatg ctcgacgact acggcggcag cctgagcgaa ctggcacggg
    15901 atcaactgcc cgctgcggaa caatcggcgt tggcgcaact ggctgcgtgg ggaattgtgc
    15961 cgcaaccggc taatgaatcg cgctgtctca attatgactg tctgtcgatg gaaaattacg
    16021 aaggcgttcg tttgcgccag tacaggatga cactggagga gcagggatga gcaacgacac
    16081 gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc tggacgccgg tcagtgaatc
    16141 ccgtcttgac gactggctta cgcaagcgcc agacggcgtg gtgttattaa gcagtgaccc
    16201 gaaacgtacg ccagaggtta gcgataatcc ggtaatgatt ggcgaattac tgcgcgagtt
    16261 tcccgactat acatggcagg tggcgattgc tgaccttgag cagagcgaag ccatcggcga
    16321 tcgctttggc gtctttcgct ttcccgccac attagtgttt accggcggaa actatcgcgg
    16381 tgtgctgaat ggtattcacc cgtgggcgga actgataaac ctgatgcgcg ggcttgtcga
    16441 accgcagcag gagcgtgcct catgagcgaa acttttttcc atctgctggg gccaggaacg
    16501 caaccgaacg atgacagttt cagcatgaat ccactgccga tcacctgtca ggtgaatgat
    16561 gaaccgagta tggcggccct ggaacaatgt gctcacagcc cgcaggtgat tgcgctgtta
    16621 aacgagttac aacatcaaat gagtcaacgc cagccgccgt tgggcgaggt gctggcagtc
    16681 gatctgttaa atctccacgc cgacgatcgt cactttatca atacccttct cggggaaggg
    16741 gaagtgtcag tgcgcattca gcaggctgac gacagtgaaa gtgaaataca ggaggcgatc
    16801 ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa agttgctgga ggacaaactg
    16861 gaagctggct gtgcgccgct ggcgttgtgg caggcggcaa cgcaaaatct cttgccgaca
    16921 gattcgctgt taccgccgcc cattgatggc ctgatgaatg gcctaccgtt ggcgcatgag
    16981 ttactggcac atgtacgtaa ccccgacgcg cagccgcaca gcattaatct gacgcaatta
    17041 cccatcagcg aggctgatcg gctttttctc tcacgtctct gtgggccggg aaatattcag
    17101 attcgtacca ttggctatgg cgagagctat atcaacgcca cggggttacg ccatgtctgg
    17161 catttacgct gtacggacac cttaaaaggc cctttactgg aaagttatga aatctgcccg
    17221 ataccggaag tggtgctggc agcgccagaa gatttggtcg actctgcgca gcggcttagc
    17281 gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa aagacggtaa gtatcgcttt
    17341 cagtcttatg aatatcgcaa tcggcgaata cctctggtcg tagagtttca ggataaagag
    17401 ggagatctac cattatcggg ttatttttct ctcttcgcct acaggagtgc gcatgtggga
    17461 tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg ctgtatcact ttttatttgt
    17521 accccttacc ctggggctga tttttttgct ggctatcatg gaaaccattt acgtggtcac
    17581 cggcaaaaca atctaccgcg atatgacgcg cttctggggt aagctcttcg gtatcaattt
    17641 tgctcttggc gtggctaccg gcctgaccat ggagtttcag tttggtacta actggtcatt
    17701 ctattccaac tatgtgggcg atatttttgg cgcaccactg gcgatggaag cattaatggc
    17761 cttcttcctc gaatccacct ttgtcgggct gttcttcttc ggctggcaac ggctgaataa
    17821 ataccagcac ctgctggtga cgtggctggt ggcgttcggt tcaaatctct ctgcgttgtg
    17881 gatattgaat gccaacggtt ggatgcaata cccgaccggt gcgcattttg atatcgacac
    17941 cctgcgtatg gagatgacca gcttcagcga gctggtcttt aacccggtca gccaggtgaa
    18001 atttgtgcac accgtaatgg cgggctacgt gaccggggcc atgtttatta tggcgatcag
    18061 cgcctggtat ttactgcgcg gacgggagcg cgatgtcgca ttacgttcgt ttgccatcgg
    18121 ttccgtcttc ggtactctgg cgattatcgg taccctgcaa ctcggagaca gttctgcgta
    18181 tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg gaaggggagt ggcaaacgga
    18241 acctgcacct gcaccgttcc atgtggttgc ctggccggaa caggatcaag agcgtaacgc
    18301 ctttgccatc aaaattcccg cgctgctagg gatcctcgcc actcactcat tagataaacc
    18361 cgtgccgggt ctgaagaatt tgatggctga aacctaccca cgcttgcaac gcggacgtat
    18421 ggcctggctg ttaatgcagg aaatatcgca aggcaatcgt gagccgcatg tgttgcaggc
    18481 attccgggaa ctggaaggtg acctgggcta cggcatgttg ctctcccgct atgcgccgga
    18541 tatgaatcat gtcacagccg cacagtacca ggcggcgatg cgtggcgcga tacctcaggt
    18601 tgcgccggta ttctggagtt tccgcatcat ggtgggctgt ggttccctgc tgctactggt
    18661 gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc gaccagcatc gctgggtgct
    18721 gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg attgaagccg ggtggtttat
    18781 gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc ttaccgacat actccgcgca
    18841 ctccgcttta accacaggac aactggcttt ctcactgatc atgatcgtag ggctttacac
    18901 cctgttctta atcgccgaag tctacctgat gcagaaatat gcccgtctgg ggccgagcgc
    18961 gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg agaaaatcat gtttgattat
    19021 gaaacattgc gcttcatctg gtggctgctg attggcgtga tcctggtggt ctttatgatc
    19081 tccgacggat ttgacatggg gatcggctgt ctgctgccgc tggtggcgcg taatgatgat
    19141 gaacgccgga tagtgataaa cagcgttggt gcacactggg aaggcaacca ggtctggttg
    19201 atcctcgctg gtggggcatt atttgccgcc tggcccagag tgtatgcagc ggcgttttcc
    19261 ggcttttatg tggcgatgat cctggtgctg tgctcgctgt tcttccgtcc gctggccttt
    19321 gattatcgcg gaaaaattgc cgatgcacgc tggcgtaaaa tgtgggatgc cggtctggtc
    19381 atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct tcggcaactt gttgctcggc
    19441 gtgccgtttg ccttcacacc gcaattacgc gtggagtatc tcggcagctt ctggcaactg
    19501 ctgacgccat tccctttatt gtgcggattg ctcagccttg ggatggtgat tttgcaaggt
    19561 ggcgtctggt tacaactgaa aactgttggt gtgattcatc tgcgttcaca gctggcgact
    19621 aaacgcgctg cactgttggt gatgctgtgc tttttgctgg cgggttactg gctgtgggtc
    19681 ggtattgatg gctttgtact gctcgcccag gatgctaacg gtccttccaa tccgttaatg
    19741 aaactggtgg cagtgctacc tggtgcctgg atgaataatt tcgtcgaatc gcccgttttg
    19801 tggatcttcc cgctgctggg gttcttttgc ccattgctga cggtgatggc gatttatcgt
    19861 ggtcgcccgg ggtggggatt tttgatggcg tcattgatgc aatttggcgt gattttcacg
    19921 gcaggcatca cgctgttccc cttcgtcatg ccgtcaagcg tgagtccgat ctccagcctg
    19981 acgttgtggg acagcacttc cagccagctg acgctgagca ttatgttggt aatcgtgctg
    20041 atatttttgc ccattgtgtt gctctacact ctctggagct actacaaaat gtgggggcgc
    20101 atgacaacag aaactctccg ccgtaacgaa aacgagttgt actaaggagc aggaacaatg
    20161 tggtatttac tttggttcgt cggcattttg ttgatgtgtt cgctctccac ccttgtgttg
    20221 gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg cctgatgcgg cgcattagca
    20281 tcgcatcagg caatcaataa tgtcagatat gaaaagcgga aacatatcga tgaaagcgat
    20341 cttaatccca tttttatctc ttctgattcc gttaaccccg caatctgcat tcgctcagag
    20401 tgagccggag ctgaagctgg aaagtgtggt gattgtcagt cgtcatggtg tgcgtgctcc
    20461 aaccaaggcc acgcaactga tgcaggatgt caccccagac gcatggccaa cctggccggt
    20521 aaaactgggt tggctgacac cgcgcggtgg tgagctaatc gcctatctcg gacattacca
    20581 acgccagcgt ctggtagccg acggattgct ggcgaaaaag ggctgcccgc agtctggtca
    20641 ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa acaggcgaag ccttcgccgc
    20701 cgggctggca cctgactgtg caataaccgt acatacccag gcagatacgt ccagtcccga
    20761 tccgttattt aatcctctaa aaactggcgt ttgccaactg gataatgcga acgtgactga
    20821 cgcgatcctc agcagggcag gagggtcaat tgctgacttt accgggcatc ggcaaacggc
    20881 gtttcgcgaa ctggaacggg tgcttaattt tccgcaatca aacttgtgcc ttaaacgtga
    20941 gaaacaggac gaaagctgtt cattaacgca ggcattacca tcggaactca aggtgagcgc
    21001 cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca atgctgacgg agatatttct
    21061 cctgcaacaa gcacagggaa tgccggagcc ggggtgggga aggatcaccg attcacgcca
    21121 gtggaacacc ttgctaagtt tgcataacgc gcaattttat ttgctacaac gcacgccaga
    21181 ggttgcccgc agccgcgcca ccccgttatt ggatttgatc atggcagcgt tgacgcccca
    21241 tccaccgcaa aaacaggcgt atggtgtgac attacccact tcagtactgt ttattgccgg
    21301 acacgatact aatctggcaa atctcggcgg cgcactggag ctcaactgga cgcttcccgg
    21361 tcagccggat aacacgccgc caggtggtga actggtgttt gaacgctggc gtcggctaag
    21421 cgataacagc cagtggattc aggtttcgct ggtcttccag actttacagc agatgcgtga
    21481 taaaacgccg ctgtcattaa atacgccgcc cggagaggtg aaactgaccc tggcaggatg
    21541 tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt tttacgcaaa tcgtgaatga
    21601 agcacgcata ccggcgtgca gtttgtaatg cataaaaaag agcattcagt tacctgaatg
    21661 ctctgaggct gatgacaaac gaagaactgt ctaatgcgta gaccggaaaa ggcgttcacg
    21721 ccgcatccgg ccactttcag ttttactctt tctcggagta actataaccg taatagttat
    21781 agccgtaact gtaagcggtg ctggcgcgtt taatcacacc attgaggata gcgcctttaa
    21841 tattgacgcc tgcctgttcc agacgctgca ttgacaaact cacctctttg gcggtgttca
    21901 agccaaaacg cgcaaccagc aggctggtgc caacagaacg ccccacgacc gcggcatcac
    21961 tcaccgccag catcggcggc gtatcgacaa tcaccagatc gtaatggtcg ttcgcccatt
    22021 ccagtaattg acgcatccga tcgcgcatca gcagttcaga cgggttaggt ggcacctgac
    22081 cgcgagtaat cacatcaaag cctcctttgc caaaatgctg gatcactttg ttgagctcat
    22141 ctttacctgc cagatattcc gacaagccat gttcattact cacggtaaac aggttatgcg
    22201 aataaccacg gcgtaagtcg gcatcaataa ataacacttt ttgatcggac tgggcgatca
    22261 ccgctgccag agttgaactg acaaacgttt taccactgtc tggcgtcgca ccggtgatca
    22321 tcagaatgtt attctccgtc tccatcatag cgaaatgcag actggttcgt agcgcacgta
    22381 cggcttccac agcagaatcc gccgggttat ccaccgccag gaaggggata tttttagtac
    22441 gatggcgctg ctgattagaa aataaatttt tcttacgcag acgggtgcgt ttatccagcc
    22501 actcggacat tgggatagtg gcataaacgc tgatgccgtg ctcttccagt tgttccgggg
    22561 cttctacacc acgacgcaac atcgcacgcg ccagcacggc acccacagaa ataaacaggc
    22621 caagaataaa accaagcacc acattcaacg cttttttcgg tttcactggc tgcggctgag
    22681 tgactgccgg gtcgataatc cgcacgttac caatggcact ggatttcgaa atactcaact
    22741 cctgctggcg gttaagtaat tgcagatata ccgcacggcc cgcttctacg tcacgactta
    22801 aacgcaacac ttcctgttgg gtggaaggca ttgccgatac ccgcttattc aggcgtttgc
    22861 gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg ataagttggg tgatctttct
    22921 tatacagctg ggagatctct gcctcgcgga aggtcagctc attgagttga ttatcaacgt
    22981 tcacaatctg ctcaagaacg gctttggctt ccaggttaag gtcaaccgaa tcgcgctgct
    23041 ggcgataaac gttgagtttt tcttccgcct ggtccagctc gctgcgcact tcaggtaact
    23101 ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc ctggcgagcg atattctgtt
    23161 gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat caactgggga tcatcaccag
    23221 tcatggtaag ttccagcatc ccgctttctt tactgcgttc gctaacggta aaggtttcct
    23281 gcaatgcgtt aatcgcttcc agttcggtac gctggctcag gacaaactgt gttcctggtt
    23341 tggccttaat gtccgcaata gtcagcgcaa cgccatcttt ttccagacgc tggccgacca
    23401 taccattgac ggtgaactct tcaccttcca gtgtatagtg gccgttttcc ccaaccgtga
    23461 gtgtcagttg ctgatcctga ccattcagtt gtggaatatg catccagctg atcgccagct
    23521 cacctggttt ttctttggtt aatctcgccc agccgcgacc cacaatcgga aaatacttct
    23581 gctcaactat gtcgcgcaga ttcagttcag caatggtttt accgagaatc atgcgcgatt
    23641 gcagcagttg gatctccggt gcagactcgg gcgatgagtt agggatcata tcgctcaggc
    23701 cgctgagaat ggcgttgccc tgtttttgct caacctggac cagagtatct gcctgataaa
    23761 ttggtgtact taacagcgag taagcgacag cgatcagcgt gaataacgcg gtcacgctga
    23821 taataaactt acggtgatcc cataactcgc cgaccagacg aagcagatcg atctcatttt
    23881 cctgagtgct gcctggtggc gtattcatat ttttagttgt catcgtattt tataaactca
    23941 aattaccggc tgaggcgctt cgcccattcc tgactggcgc gctccaacat accgtagaca
    24001 tgttcaaatg cgtcctgact tttacgatag ggatccggga tctctttctg ttccagccat
    24061 tgcccaaaca gcattgtctt cccgcggacc tcgggagcaa ttgcggtaac ctgagcaata
    24121 tgttccgact ccattgccag aatcagatcg taatttcgtg ccatctcagc agtgagctta
    24181 cgtccggcat gtccttcaag agatacgcca tgattagcgg caacatctgc cgctgtcgca
    24241 tctgcagggt gttttaccag accatgaaca cctgccgatt tgacttttac gcccggcaga
    24301 cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa tgttgccggt acaaaccacc
    24361 aggattgagt taaattttag ttgggccatg tttgaatcca ccgtgaagtc tctgtcaggt
    24421 catgcacccc ggaaatcgtc ggtaccagca gagaaatcac acggttccaa cgagccagcg
    24481 gcgcggtagt gacataaaca atgtcgtaag gttcaagctg gaattccgtt ccgagtatca
    24541 ttgccgatgc gtccttcgca ttcagctgat aaatattggc gattttgcca ttctgcttgt
    24601 tttgcgttgc acgaataaca aaaatccccg tcgcatcagc gacatcctga ttcataccct
    24661 cagcgttacc cagcgcttcc gccagggtca taccgctgcg gtccattttc agcgtgctct
    24721 gtttacccac ttcacccatc acgaagactt tcagatcatc gttgcgcgga ataaataaaa
    24781 tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc acgctgcatc agcgcataga
    24841 ggttcacttt ggtctttacg ccattctggg tgagcaccac gttgcgccag tctgcatctg
    24901 cggtcagacc gcccgcggcg ttaatcgcat ccatgatggt cagaggaata ttggtaatcg
    24961 gctgctgacc cgatttgctg acttcgccgg tgacgtacgc tttttgcgag cggaacgccg
    25021 cgacgctgac atcgacttgc gggctttcaa tgactgaatc cagacgcgct gtaatctcgt
    25081 tgcgtacctg ggtaagtgtt ttcccggcca ctttcaggcg accaatatag gggtagaaaa
    25141 tcgcaccatc ggcgttaacc cagttgccgg tatcactggc gctacggtat tgccctgcgg
    25201 gggtggttag ttccgggtga tcccagaccg taaccatcaa cacatcccca atgccgatac
    25261 gatattcata gtttgcgatt tgctgctcca gctccgggtt agcctgcgac ataatcggtt
    25321 tggcgcgcaa ctgatcaatc aatcctggcg tgacgggata gatattgacc attttgtcca
    25381 ggtcatactg attgtcaggt agttcaatga catccttgtt actggtcgat aaattctgac
    25441 caggaacaag ggtacatgct gtcaaagaca gaacagccaa tgtcagtacc gaaaacttaa
    25501 aaatgttctt tttcattggt ttatatttat cagtcaatgt aatgttatga attacgtcag
    25561 gttattgtcc aaccggtact tctcgctctg ccgtcattgg atagagctgg tatttgcgtg
    25621 acagcatttg tccaccatca cgcgtcaatg gtgtccacga aaccaccgcg cggttgcggt
    25681 taggtccaat ggtcatcaag tcgaatggaa tcgagatata aaaaccttta ctaaagccgc
    25741 cttcgccgta gtcatcttta gatacgttac tgattgccgc ccatacccct accgccacgc
    25801 cgctgtcaaa gcgtttggcg acgtcgatcg ttgcgccttt atcttttgcc agatattgcc
    25861 caacgctaag tttcatcagt acgccattga gcgtcggcgg gttccagtaa gccgtcacga
    25921 aacctgttgg cgtggaataa tcggtgaaac gcatcatgtt gtcccagtca cgttgcttca
    25981 cgtagttaac gtccacaccc agcgcccagc tggcatctag cgggcgataa agcagctcgg
    26041 aaccgacacc ggcgtacatc gtttccagat aaccgccata cacctggcca tagaaaccat
    26101 tgcctaagtc ggcaaagtag ttcgcctgca agttgttgag ataaacgtca ttgcgaacgt
    26161 aatcacggat atgcgtgcgc acgcggggca gggtagagtc cgcgggcaac agcgaagact
    26221 taaacttgtc gtagttgttg taaatattgg tgaaaatacc gccatcaagc agcaggtggt
    26281 cggtaaacca gtagcgggca ctggacatca gccccagctg gaacatatag aaatcttccg
    26341 ggccgcccag cgactgtgaa agtgttgggt tgaaagagta gctaaagcga tcttcacgaa
    26401 tacggtagcc ccgaccaaag gcagaaagat cttctgcttc aacacgttgt tgttgaagtt
    26461 gctctgattg accaggcgct gtaccagcca gctgtttgcg caggctggct acgtcggttt
    26521 cggtagtcac catcgccata tgctcgcgct tttgcgtcac gctaatcttc tcaacgcctt
    26581 gcggcaggtt attcaccaga atccggttgg cacgatcgac cgcttcgcga gaatcacggt
    26641 atttgtattg ttgaccagac atatacagcg tcttatcgcg cagctgaatt tccggtgctt
    26701 caaaacctgc gttgtacttc agcgcggtaa gttggttcgc tactgtggtg tactgcaatc
    26761 cttcagattc aggcgcaggt tgatatgccg gttttggcgt atcgcgcagg gcagggcgca
    26821 gatcgttgaa attggtccgt aacgtgaagc caaacatcaa cgtgttaccg cgttcataac
    26881 tcaggttgag atctgcccag ctggcagcgc gataaactgc gccgacgttg aaatggcttg
    26941 cctgaggcag tttgccagcg aaatcattct ggtaattgtt gccatcgtat tcgagtttca
    27001 gacgcagggg attccacggc gtttgatact caatgccgcc aaagatggaa gccgggccac
    27061 gaaagatatc gctaaagctg atatcgcccg catcgtgaga ctctgcgcga tgacaatatt
    27121 tatcgctcac ccggcaaaac gggttggtaa tattgcccgc attaccggcg tagccccatg
    27181 ccatcccgag ggtgaaatca aatggccccg cttgcttgct ggccaccaga tactcaccgt
    27241 caaacagacc cgtaccagca atatcacgtt taccaaacgc cacttgcggt agccagtaac
    27301 cttcttccca caggcgaagt ttaaaatcga atgatttgtc tttgtatgac tgatcgccgc
    27361 tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc cgtataacga atggtgcctt
    27421 ccagccatgg gaacagcgcc acggaggtgg agtagaaccg gtattgatcg ttatcccggt
    27481 agttgacgct gaattcacct tccggtgcga tgcgcgcatt tggcatctgc aacaatcctg
    27541 tgccgccaaa gtctgattgc gacgaaccca gcggatccgg gtaggttaat acttcagcat
    27601 gacaggcact ggagacggca atggccaggc agcttaaaag ataagaattc ttcttcatca
    27661 gtcaggaaca cgttgcgtta aaacagacac aatctggtca tttaaatcag catatttttc
    27721 gggcagtaca tgcgcagaaa agcccaacca aagctggctg ccaggcggtg gttccacatg
    27781 tcttttattc cacagggcaa caggggcgac gacagtctca ccctcaggcg taatcaccat
    27841 aacgttattc ttgtccgcgc ctgcaaggcg ggggtgatct tgcagataat cggtaacgct
    27901 acggcctgcc tgccagggca attgcccggc cccagaaacc gcacccagca gagtaatagt
    27961 caccggacgc tgtacggtat agagcgtata gtccccgacc aacgggggat tgctgttctc
    28021 atccactcgt acaaaatcag ggtccagttt gaccggtaag cgaccagtga tattgagatt
    28081 gagtaactgc tggcgcacgg atttaatcgt tgccgccaca tcgtcgtcag cttctgcttc
    28141 ccaggaagca agctgtgcca tgacatgttg gtagtcttta agcgctttag ctttcgccgc
    28201 agagtcggtc agcaaggcac caggccacca gaggcgatcg cgcagttgcg gttgtgtcac
    28261 caactggaca acattttcca tggggccaac ggaaagtgtc tgttgttcgc caggcagata
    28321 aatagtcacc gttccttgcg caaaggcatg gggtgtcatt acgtaaagta cgctggcaat
    28381 gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa acgtcatttc tacgggaata
    28441 acccctgcgc ctaacatttg ccgactttga cgaacctggc cagtagccga atcgacccag
    28501 aatgtgtttt gccactgttt ttctggccgc gtggaagtca cctcttcctg ccagacatta
    28561 cacagcacat tgcgacccgc aatattcagc gtctctaatc cagcaaagga aaaagtagaa
    28621 ctcagggtcg cagagcgaaa atggttgtct tcactccaga gaatatcgcg cgtccagcgg
    28681 ctgccatctt ttattgccag cgcgttacgc agagggtcct gcccggaatt tgtcacttcc
    28741 agcagattat tattaagttt gacggttttt aaaagacgtc cattgtgggt aaccagcatg
    28801 gcgttatcct gggataacca tttgctttgt tcttgttcta tatatcccag taccacaaag
    28861 attcgttgcc cttcattaag gcgtaaatac atagtggaat aaggcaacgc ctggatctgc
    28921 tgatccgcta cggtgatatc ctgattatcg aaaaggctgg cgcgaaatgt atcgaccata
    28981 ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga aaatcgataa aataagaggg
    29041 cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa atctctaaag cccggtacga
    29101 atatcgcaac cgggtatcat ttaactttaa tttcgacgcg ttaaagcagt attagcgtgt
    29161 agtggacgtg gtggttgagg tgttatgaga accgccatcg ccactgtcat ttgctgcaac
    29221 aactgccaac gtcgccacac caacagccgt tgccacaccg acaactgtcg ccgtgccgac
    29281 agcgccagca gccacgccac cagtggtagt agcagtcgtg gttgcgccgg tagtggtcgt
    29341 tgtggtgcca gtagtacccg tcgttgcctc ggtcccgttt gttgcttcag gggcagcaaa
    29401 cgcagctggc gtcgtcaaca tgaaggccat aaggattgca gaaagcttgt gtttcataac
    29461 ttttccttta ttcatcgcat ggacaatacg tacgcttgag atcaggattg tctactttga
    29521 gtataacatc cttgtgaaat ctccctttcc tcataccgct tgcactatat actgcgggta
    29581 ggaaaggcgc gcagagggaa atataagatt gttaactatc cccctctgga aatacgactt
    29641 gtgagcataa gataaatctt agcaaaatac aaattaaaga taaggaaatt gttacgaaag
    29701 ctattaatta ttgttggtaa tatagtttca agtggtacta taactggctg ttaaatggca
    29761 tttataacta ttaggtgcag agatattcgc ttaaatggat tagtttcttt gtggaaaagt
    29821 aactgatgtt ataatgtatg atgttgacta ttgcactatt ccaataaaac cagtcagctt
    29881 taaacaagca gcgtcatatt aagagagata aacatttgcc gctgttggtc ctcgcaggcc
    29941 atttacgcgg caaaattcca cacgtaatcc tggtataagc acttctgcgt cgcggggagt
    30001 gaatgcggaa atatggacct gaacttcttt acgaccgtcg gaggggataa tgaatccttt
    30061 gccgcttttg cgatcaaagg ttttgacaat tcctgtcatt ttacgggaca aacaaattcc
    30121 ttactgaaaa tactgcgctg cactatacgg ggttaataaa ataaagccag cgatatttaa
    30181 gaccgccgga cggctaaaat aaaatttgct taatctcaat tatcatgcgt taatagctgc
    30241 gtcggtttga aagacagaca gcatacaaag tagtttacta aagcagttct cattatcagg
    30301 cattatcccc ttcttttgag tctctctcct gaacactaag tagtttctgt attaaagccc
    30361 tgtttgccga aaggcccaaa atgaaggaag taaaatatgt ctaataaaat gactggttta
    30421 gtaaaatggt ttaacgcaga taaaggtttt ggctttatca ctcctgatga tggcagcaaa
    30481 gacgttttcg tccatttcac cgccatccag agcaatgaat tccgcacgct gaacgaaaat
    30541 cagaaagttg aattttctat tgagcagggg caacgtggcc ccgcggcagc gaacgttgtt
    30601 acgctctaag gttgccgtta ttactcaaca tctccatttc cgctgtccat gttgtcatgg
    30661 ttcacagtac cgcacatcgg cattcgatgt gacggagcga aaccctttgg gcgctaagtg
    30721 tattttttgt aaatcgacga tgatcacctt tgataacgtc gcgctgcaaa tacgcactga
    30781 ccatgcgccg ctggatttca caaaataata ttaggctccc tcgtggagcc ttttttatat
    30841 ctgccttatt tttcttcaac gctgtatgta tagtaagcga taacctgttg attattgaat
    30901 ctttcgggga gatggcttat aacatttctt acctgaccag ggtaccggga accaacacct
    30961 tactggcgtg ttgctgtctt ttaagaccag aagaggttaa cagtgaatat tgaagagtta
    31021 aaaaaacaag ccgaaacgga aatcgccgac tttatcgcgc aaaaaatcgc cgactttatc
    31081 gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc
    31141 accgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
    31201 ttttgtgaac atcacccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc
    31261 attggcgctt gtggaattaa gcgtcggtac aattcatccg gtaccgggct ttgccatact
    31321 cccgcataca ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt
    31381 aaccagccag acaacgcaaa ggctttttta tttgccgcag gtttttgcag tgaaaaggtc
    31441 aatgttgagg ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt
    31501 cgtacctgaa ttaatttatc gaccgggatc cgtgatgggc aattttttgc gcatttgcca
    31561 cagtcgatac aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg
    31621 cccatcagcg cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg
    31681 gcaatcagca aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg
    31741 agcattttaa cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga
    31801 gcgggcatca ataatgcgat atagagaaaa aaactcaaca gcaaatactt cacgccgcgc
    31861 agaggaatat ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga
    31921 tcgccgatta attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg
    31981 agtaatagac tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac
    32041 ggtaactgac ctgttgtaag gctatatttc agattcatca gaccggcaat cggtagccag
    32101 ccttcgatac ctcccggtct ggcgacaaat gtcgtgctac ttgccgtttc gtaatagcgc
    32161 acccaatacc agaacgtgat ggcaatataa atattcattg ccaacagtaa taattgcgtc
    32221 gctttacgcc aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg
    32281 cctggccgcc gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa
    32341 gttgcatacc aaatgccact attctagttg ttcttaactg gctgatattg attcaaattg
    32401 cgttcaggtc tttcttattc aaccatgctt ccagatcggc aacactgcgt gtaatttctt
    32461 cgtgtggaag gggggcagat aatggctgct gctccagttg tgcgcatagc tggctggcga
    32521 tacgcattcc caaactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct
    32581 cgctatcctg actggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtg
    32641 taaataatac cagccattcg tggatcttct ccgtccccat taactgagca tcttcattga
    32701 gttgcgatac atccagcgat tgatcgttat tgacttgcag ttggaaatag tgcgccagta
    32761 actgaccgag cacttcacgc ggcaccggtt tagggataat cccgcggaat aatgaactgg
    32821 tacgctggcg cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg
    32881 gatattgctg tgccagttgt cgggcaagcg taatgccgtc gacatccggc agatcaaaat
    32941 ccaccagtgc ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat
    33001 tgccaacagc aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg
    33061 ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattga
    33121 ctgtttttgg cacgggtgcc gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc
    33181 caacctccgg cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac
    33241 tgatagtcag ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga
    33301 agatttctgc cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc
    33361 attgctcgcc atcagtgcga ctacgcagga taatgtaccc ttcgtcagta aaacgcaggg
    33421 cgttgctcaa caggttggtt ataacctgac gaatacgtcg tggatcgccc attaacgcgc
    33481 acggcatatc atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc
    33541 tcattaattg cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgaccg
    33601 aaacattctt gccacctgct tcgatagcgg aataatcgag aatatcgttg aggatggtca
    33661 gcaacgattc accagagtca gtaattgccc gcaaatcatc acgctgggcg ttaagtgcgg
    33721 ggttatctgc cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat
    33781 ggctcatcgc cgccagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg
    33841 cctgtcggtg ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg
    33901 ccagctgttc gcggtggcga ttcagtgcat gaacattgct gcgaaacgca tccatcagcc
    33961 gcccgatggt atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac
    34021 cgtccagcag ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg actgagcgat
    34081 aaaccacgcg ccaaaggatc agaatcagtg cgcaaagtga aaccatcccc agcaataaca
    34141 ggctgtattg cccgcgtgca ctggcttttt ccagatgcgc cagtccgtgc tgattacgca
    34201 gctcaatggt gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatgt
    34261 tattttgtgc gagagtttgt aggtgattgc tgatttcact gtcctgctga tacagcgcca
    34321 gcaaatcgct atattggcta acggtagtta acgttgttgc gacctgcgca cgaacacccg
    34381 gatcttcaat gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt
    34441 ccagcgttgg tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca
    34501 cccgcagagc gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa
    34561 tcagccgatc gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa
    34621 tcccggcctg ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgcgccaggc
    34681 gtgcgatctc atcggcggca gcgactatct gctgactgag ttgccgttgt tgctggcgta
    34741 gttgcagacg ccgccccacc agttcccctt gctgacgtaa tgaacgggag atctcctgct
    34801 cctgttgttc aatagcggtg gtatcaaaac cttgttcccg taacgcttgc agcaacgcat
    34861 taatcttcag gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat
    34921 cggcactggt caggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc
    34981 gcgccatatt catggtagga atcaatgcct gagtgttgtc tttttccacc tggctgataa
    35041 agcgcaggtt gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa
    35101 agcccatcca gagtcttcgg gtcagggtta aattcacggt cggtgcactt aaggtgaaaa
    35161 aggttgagtc gcaaagcgga atgcatctag cataaagcct tattattgat gaggctatca
    35221 tgcgcgtact gctattttta cttctttccc ttttcatgtt atcggcattt tcggctgata
    35281 acctgttgcg ctggcatgat gcgcagcatt acacggtgca agcctctacg ccgcttaaag
    35341 ccaaacgcgc atggaaactg tgcgcgcttt atcccagcct gaaagattca tactggttat
    35401 cgttgaacta tggtatgcag gaggcagctc gccgctatgg tgtggattta aaagtgctgg
    35461 aggcaggcgg ctacagccag ttggctaccc agcaagcaca aatcgaccag tgtaaacagt
    35521 ggggcgcaga ggccattttg ctcggtagta gcactacgtc atttcccgac ctgcaaaagc
    35581 aggtagcaaa tctgccggtg atcgaactgg taaatgctct tgatgatccc ctggtgaaaa
    35641 gccgcgttgg tgtaccctgg tttcagatgg gctatcaacc tgggcgatat ttggtgcaat
    35701 gggcgcacgg taaaccactg aacgtgctgt tgatgcccgg acccgataat gccgggggca
    35761 gtaaagagat ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg cgtattgttg
    35821 atattgcgct tggtgataac gatattgaaa tccagcgtaa cctgttgcag gagatgctgg
    35881 aacgccaccc agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg
    35941 gtgaagggcg taacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg
    36001 tgtatcgcgg gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa atggtctggc
    36061 agggggaact ggcggttgag caggccatca ggcaattaca ggggcaatcg gtgtctgata
    36121 atgtcagccc accgatttta gttctgacgc cgaaaaatgc cgaccgtgaa catattcgcc
    36181 gctcgctgtc accaggggga tttcgtccgg tctattttta tcagcacaca tcagcggcta
    36241 agaaataacc ttcaccatgt tgcgtcacca gtaaatccgc gctgagttta tgacgtaaac
    36301 gacgaattaa cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagaaagca
    36361 tacgtagcag acgttcacgg ctgagaattt cgcccggatt cgtcacaaat gccaccaaca
    36421 tttcatactc tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg
    36481 acacattcag gcaataaccg gcaaagcgat agcagttgtc ctgagtgtgc ggttgagctt
    36541 gtcgcgcgag gtcgattcgc cagagcagat ttttcacccg tactaccagt tcgcgcagtt
    36601 ccagcggttt ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc
    36661 gatcgctgcg tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg
    36721 ttaacatcag gccattttca tcgggtaagt tgatatccag cagaattaaa tctaccggct
    36781 gattctgcat aatttcccgc agcccggcac cgctcgctgt aacggaaacg gtatacccct
    36841 cctgagtgaa gtaagattgt aatcgcgcct gggtaaccgg ctcatcttca acaataacaa
    36901 tgtgatgtgg catcagaggg ttttactcat tctgttcata tctgttcata ttctgccgta
    36961 agccgttcat cctgaccagt gccgctgttc atatttgctc attaagatcg cttcactaaa
    37021 ccataatttt acaggggcta ttatgcggaa actctggaac gcgctacgcc gacccagtgc
    37081 tcgttggtcg gtactggcgc tggtcgcaat tgggattgtg attggcattg cgctgattgt
    37141 attgccacac gttgggatca aagtcaccag cacaaccgaa ttttgtgtca gttgccacag
    37201 tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat ttccagaacg cctccggcgt
    37261 gcgagctgaa tgccatgact gtcatatccc gccggatatt ccaggcatgg tgaagcgcaa
    37321 actggaagcg agcaatgata tctaccagac ctttattgct cactccattg atacacctga
    37381 aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa tgaaagaaaa
    37441 caactcggca acctgccgct cctgccataa ctacgatgcg atggatcatg cgaagcagca
    37501 tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat aatcaatcct gcatcgactg
    37561 tcataaaggt attgcccacc agttaccgga tatgagtagc ggcttccgta agcagttcga
    37621 tgagctgcgc gccagtgcta atgacagtgg tgacacgctg tactctattg atattaagcc
    37681 aatttatgcg gcgaaaggcg ataaagaagc ctctggttct ctgctgcctg cttcggaagt
    37741 gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa attaccggct ggacggaaag
    37801 cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg ttgcctcgat
    37861 tcgtggtgat gtgcagcagc aggtaaaaac gctggagaaa accaccgttg ccgacaccaa
    37921 taccgagtgg agcaagttgc aggccactgc gtggatgaag aaaggcgaca tggtgaacga
    37981 tatcaaaccg atctgggctt atgcggattc gttgtacaac ggcacctgta accagtgcca
    38041 cggcgcaccg gaaatcgccc actttgacgc taacggttgg atcggcacgc tcaacggcat
    38101 gattggcttt accagtctcg ataaacgtga agaacgcacc ttgttgaaat atctgcaaat
    38161 gaatgcgtct gacaccgcag gtaaggctca cggcgataag aaggaagaaa aataatgaac
    38221 aataacgatc tctttcaggc atcacgtcgg cgttttctgg cacaactcgg cggcttaacc
    38281 gtcgccggga tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc ggcgcaagcg
    38341 gcgactgacg ctgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
    38401 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
    38461 ccgtcgaaaa tgattgccgg attgccggat cacgtacaca acgcggcgcg tattcgttat
    38521 ccgatggtac gcgtggactg gctgcgtaag cgccatctca gcgatacctc ccagcgcggt
    38581 gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg
    38641 gaacgcgtgc agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg
    38701 acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat
    38761 agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatcct gccgcgcgtg
    38821 gttggctcaa tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc
    38881 aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc agcaagcgaa ctggtggtgc
    38941 ccggatcacg atgtttatga atattacgcg cagctaaaag cgaaagtcgc cgccggtgaa
    39001 attgaggtca tcagcatcga tccggttgtc acatccaccc atgagtatct ggggcgcgag
    39061 catgtgaagc acattgcggt taacccgcaa actgatgtgc cgctgcaact ggcgctggcg
    39121 tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtgggt
    39181 tttgagcagt tcctgccgta cctgctgggt gagaaagacg gtcagccgaa agatgccgca
    39241 tgggctgaaa aactgaccgg cattgatgcc gaaagcattc gtgggctggc gcggcagatg
    39301 gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca gcacggtgaa
    39361 cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt
    39421 ggtggctttg gttttggctg gcactataac ggcgcaggca cgccggggcg taaaggcgtt
    39481 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgattac
    39541 aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
    39601 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
    39661 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
    39721 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
    39781 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
    39841 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgcagttcga ggcgcgcaac
    39901 gactttgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa
    39961 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
    40021 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
    40081 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
    40141 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
    40201 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
    40261 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
    40321 tcgcagttat gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca
    40381 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
    40441 gtctttaacg ctcgcggtca ggtgttggca ggggcagtag tttctgaccg ctatgcaccc
    40501 ggcgtggcac gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    40561 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcggtac ttcgcagctg
    40621 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggagcagtg
    40681 gagcaggtga cggcgtttaa cggccctgtg gagatggtgg cgcagtgcga atatgttccc
    40741 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    40801 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    40861 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctggct gcggcggtga
    40921 atgagctgga aaactgtatt gccacgctga cagtacgtga tgatgcccgt ctggaactgg
    40981 ccgctgactt ttgcggcctg tttctgatga ccgacaaaca agcggcgctg ccgtatgcat
    41041 cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa
    41101 ccagcggcaa tttcaacgaa ccggcagatc atctggcgat ctatctcgaa ttgctcagcc
    41161 atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac agtttgcggc
    41221 aaaaaacact gacggcgctg tggcaatggt taccagagtt tgttgtgcgt tgtcgtcagt
    41281 atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagagcg
    41341 accaccaaaa cagataacgt cgtttgtgcg cctgaaaaga cgcgtttagc gtcgcatcag
    41401 gcattatggg gcagttgccg gatgcgtcat cacatccggc aaacacacat cacggatgag
    41461 ctacaaaccg ggaaagtcgc tggcgcagca ggcggttttc ctgcttcagg tgcgcaatat
    41521 catccattaa cgtcagcgcc accgcgatcc caggccagtc cagagccagt tcatgacgca
    41581 ggcgtactgc gcgttgcacc acgatggcgg catggtcgtc aaatacccag gttgtttcct
    41641 ggatctcacg cggttcaacc acccccaaac cgacaatttc attcaactcc tcttcagaga
    41701 tgccggtatg caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccattatgc
    41761 tttcccccaa tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag
    41821 cgcggcagtg ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc
    41881 gccggtctgt tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg
    41941 gctgcctggc gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc
    42001 acccagcgcc gcttcccacg ggctaaccgg caccacaatt tccagatcct ggccgacaat
    42061 atcgaacagc ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccacc
    42121 gttttcgccc ggcgtcccct ggcctttcag acggatgcgt tgaccattgc cgacgcccgc
    42181 cgggatcttc acattcagcg ttttcgggat ttcctgttcg atcatgccaa aggcgttata
    42241 aaccggcagg ttatagctga tggtacgctt atgctcagtc agcgtttctt cgaggaatac
    42301 cgccacttcg atttcaatat cgtggccgcg tgcggcgggg cgttgacggc tctggcgggc
    42361 atgctgaccg aaaattgacg agaagatatc gtcaaaatct tcggcattaa aactctgacc
    42421 gtcgccatga tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc
    42481 atactcagcg cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa
    42541 gcgggcttcg gcatccggtt ctttgctgac atcaggatgg tatttacggg ccagtcgacg
    42601 ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata
    42661 atcctttaat tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta
    42721 aggtaactcc tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt
    42781 tattgaaatc atcgcggaga ggaggtcgcc atcaagatgg gttgctgaac atattttaaa
    42841 caggtgaaaa agggtgagcg atttttgata gttgaaccag gcactttaag tttaactggg
    42901 gcgtcattat ttattaaat
//
LOCUS       DAZFVO020000022        42909 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFVO020000022 DAZFVO020000000
VERSION     DAZFVO020000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 42909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.022"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(136..811)
                     /locus_tag="ACHKNB_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /pseudo
     CDS             complement(136..811)
                     /locus_tag="ACHKNB_RS05625"
                     /old_locus_tag="pgaptmp_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(956..1564)
                     /gene="yiiX"
                     /locus_tag="ACHKNB_RS05630"
                     /old_locus_tag="pgaptmp_001126"
     CDS             complement(956..1564)
                     /gene="yiiX"
                     /locus_tag="ACHKNB_RS05630"
                     /old_locus_tag="pgaptmp_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HGU4194683.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1748..2065)
                     /gene="metJ"
                     /locus_tag="ACHKNB_RS05635"
                     /old_locus_tag="pgaptmp_001127"
     CDS             complement(1748..2065)
                     /gene="metJ"
                     /locus_tag="ACHKNB_RS05635"
                     /old_locus_tag="pgaptmp_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HGU4194684.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2342..3502
                     /gene="metB"
                     /locus_tag="ACHKNB_RS05640"
                     /old_locus_tag="pgaptmp_001128"
     CDS             2342..3502
                     /gene="metB"
                     /locus_tag="ACHKNB_RS05640"
                     /old_locus_tag="pgaptmp_001128"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGU4194685.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3505..5937
                     /gene="metL"
                     /locus_tag="ACHKNB_RS05645"
                     /old_locus_tag="pgaptmp_001129"
     CDS             3505..5937
                     /gene="metL"
                     /locus_tag="ACHKNB_RS05645"
                     /old_locus_tag="pgaptmp_001129"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HGU4194686.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6076..6210)
                     /locus_tag="ACHKNB_RS05650"
                     /old_locus_tag="pgaptmp_001130"
     CDS             complement(6076..6210)
                     /locus_tag="ACHKNB_RS05650"
                     /old_locus_tag="pgaptmp_001130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194687.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6286..7176
                     /gene="metF"
                     /locus_tag="ACHKNB_RS05655"
                     /old_locus_tag="pgaptmp_001131"
     CDS             6286..7176
                     /gene="metF"
                     /locus_tag="ACHKNB_RS05655"
                     /old_locus_tag="pgaptmp_001131"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HGU4194688.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7505..9685
                     /gene="katG"
                     /locus_tag="ACHKNB_RS05660"
                     /old_locus_tag="pgaptmp_001132"
     CDS             7505..9685
                     /gene="katG"
                     /locus_tag="ACHKNB_RS05660"
                     /old_locus_tag="pgaptmp_001132"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HGU4194689.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9779..10684
                     /gene="yijE"
                     /locus_tag="ACHKNB_RS05665"
                     /old_locus_tag="pgaptmp_001133"
     CDS             9779..10684
                     /gene="yijE"
                     /locus_tag="ACHKNB_RS05665"
                     /old_locus_tag="pgaptmp_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HGU4194690.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(10711..11328)
                     /gene="yijF"
                     /locus_tag="ACHKNB_RS05670"
                     /old_locus_tag="pgaptmp_001134"
     CDS             complement(10711..11328)
                     /gene="yijF"
                     /locus_tag="ACHKNB_RS05670"
                     /old_locus_tag="pgaptmp_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HGU4194691.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11603..12706)
                     /gene="gldA"
                     /locus_tag="ACHKNB_RS05675"
                     /old_locus_tag="pgaptmp_001135"
     CDS             complement(11603..12706)
                     /gene="gldA"
                     /locus_tag="ACHKNB_RS05675"
                     /old_locus_tag="pgaptmp_001135"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HGU4194692.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALEHVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12717..13379)
                     /gene="fsa"
                     /locus_tag="ACHKNB_RS05680"
                     /old_locus_tag="pgaptmp_001136"
     CDS             complement(12717..13379)
                     /gene="fsa"
                     /locus_tag="ACHKNB_RS05680"
                     /old_locus_tag="pgaptmp_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4194693.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13391..15892)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS05685"
                     /old_locus_tag="pgaptmp_001137"
     CDS             complement(13391..15892)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS05685"
                     /old_locus_tag="pgaptmp_001137"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4194694.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16201..17280
                     /gene="frwC"
                     /locus_tag="ACHKNB_RS05690"
                     /old_locus_tag="pgaptmp_001138"
     CDS             16201..17280
                     /gene="frwC"
                     /locus_tag="ACHKNB_RS05690"
                     /old_locus_tag="pgaptmp_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HGU4194695.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17295..17615
                     /gene="frwB"
                     /locus_tag="ACHKNB_RS05695"
                     /old_locus_tag="pgaptmp_001139"
     CDS             17295..17615
                     /gene="frwB"
                     /locus_tag="ACHKNB_RS05695"
                     /old_locus_tag="pgaptmp_001139"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4194696.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17666..19963
                     /gene="pflD"
                     /locus_tag="ACHKNB_RS05700"
                     /old_locus_tag="pgaptmp_001140"
     CDS             17666..19963
                     /gene="pflD"
                     /locus_tag="ACHKNB_RS05700"
                     /old_locus_tag="pgaptmp_001140"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4194697.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQLFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19929..20807
                     /gene="pflC"
                     /locus_tag="ACHKNB_RS05705"
                     /old_locus_tag="pgaptmp_001141"
     CDS             19929..20807
                     /gene="pflC"
                     /locus_tag="ACHKNB_RS05705"
                     /old_locus_tag="pgaptmp_001141"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HGU4194698.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVNMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20809..21150
                     /gene="frwD"
                     /locus_tag="ACHKNB_RS05710"
                     /old_locus_tag="pgaptmp_001142"
     CDS             20809..21150
                     /gene="frwD"
                     /locus_tag="ACHKNB_RS05710"
                     /old_locus_tag="pgaptmp_001142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HGU4194699.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21137..21988)
                     /gene="yijO"
                     /locus_tag="ACHKNB_RS05715"
                     /old_locus_tag="pgaptmp_001143"
     CDS             complement(21137..21988)
                     /gene="yijO"
                     /locus_tag="ACHKNB_RS05715"
                     /old_locus_tag="pgaptmp_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4194700.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22137..23870)
                     /gene="eptC"
                     /locus_tag="ACHKNB_RS05720"
                     /old_locus_tag="pgaptmp_001144"
     CDS             complement(22137..23870)
                     /gene="eptC"
                     /locus_tag="ACHKNB_RS05720"
                     /old_locus_tag="pgaptmp_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HGU4194701.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24053..26704)
                     /gene="ppc"
                     /locus_tag="ACHKNB_RS05725"
                     /old_locus_tag="pgaptmp_001145"
     CDS             complement(24053..26704)
                     /gene="ppc"
                     /locus_tag="ACHKNB_RS05725"
                     /old_locus_tag="pgaptmp_001145"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGU4194702.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27056..28207)
                     /gene="argE"
                     /locus_tag="ACHKNB_RS05730"
                     /old_locus_tag="pgaptmp_001146"
     CDS             complement(27056..28207)
                     /gene="argE"
                     /locus_tag="ACHKNB_RS05730"
                     /old_locus_tag="pgaptmp_001146"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HGU4194703.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28361..29365
                     /gene="argC"
                     /locus_tag="ACHKNB_RS05735"
                     /old_locus_tag="pgaptmp_001147"
     CDS             28361..29365
                     /gene="argC"
                     /locus_tag="ACHKNB_RS05735"
                     /old_locus_tag="pgaptmp_001147"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HGU4194704.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29373..30149
                     /gene="argB"
                     /locus_tag="ACHKNB_RS05740"
                     /old_locus_tag="pgaptmp_001148"
     CDS             29373..30149
                     /gene="argB"
                     /locus_tag="ACHKNB_RS05740"
                     /old_locus_tag="pgaptmp_001148"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HGU4194705.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30210..31583
                     /gene="argH"
                     /locus_tag="ACHKNB_RS05745"
                     /old_locus_tag="pgaptmp_001149"
     CDS             30210..31583
                     /gene="argH"
                     /locus_tag="ACHKNB_RS05745"
                     /old_locus_tag="pgaptmp_001149"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGU4194706.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32082..33296
                     /gene="manD"
                     /locus_tag="ACHKNB_RS05750"
                     /old_locus_tag="pgaptmp_001150"
     CDS             32082..33296
                     /gene="manD"
                     /locus_tag="ACHKNB_RS05750"
                     /old_locus_tag="pgaptmp_001150"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HGU4194707.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33363..34646
                     /locus_tag="ACHKNB_RS05755"
                     /old_locus_tag="pgaptmp_001151"
     CDS             33363..34646
                     /locus_tag="ACHKNB_RS05755"
                     /old_locus_tag="pgaptmp_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194708.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34898..35815
                     /gene="oxyR"
                     /locus_tag="ACHKNB_RS05760"
                     /old_locus_tag="pgaptmp_001152"
     CDS             34898..35815
                     /gene="oxyR"
                     /locus_tag="ACHKNB_RS05760"
                     /old_locus_tag="pgaptmp_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HGU4194709.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35798..37198)
                     /gene="sthA"
                     /locus_tag="ACHKNB_RS05765"
                     /old_locus_tag="pgaptmp_001153"
     CDS             complement(35798..37198)
                     /gene="sthA"
                     /locus_tag="ACHKNB_RS05765"
                     /old_locus_tag="pgaptmp_001153"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HGU4194710.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37519..38169
                     /gene="fabR"
                     /locus_tag="ACHKNB_RS05770"
                     /old_locus_tag="pgaptmp_001154"
     CDS             37519..38169
                     /gene="fabR"
                     /locus_tag="ACHKNB_RS05770"
                     /old_locus_tag="pgaptmp_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HGU4194711.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38169..38528
                     /gene="yijD"
                     /locus_tag="ACHKNB_RS05775"
                     /old_locus_tag="pgaptmp_001155"
     CDS             38169..38528
                     /gene="yijD"
                     /locus_tag="ACHKNB_RS05775"
                     /old_locus_tag="pgaptmp_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HGU4194712.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38568..39668)
                     /gene="trmA"
                     /locus_tag="ACHKNB_RS05780"
                     /old_locus_tag="pgaptmp_001156"
     CDS             complement(38568..39668)
                     /gene="trmA"
                     /locus_tag="ACHKNB_RS05780"
                     /old_locus_tag="pgaptmp_001156"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HGU4194713.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39782..39972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40037..41881
                     /gene="btuB"
                     /locus_tag="ACHKNB_RS05785"
                     /old_locus_tag="pgaptmp_001157"
     CDS             40037..41881
                     /gene="btuB"
                     /locus_tag="ACHKNB_RS05785"
                     /old_locus_tag="pgaptmp_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4194714.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41826..42683
                     /gene="murI"
                     /locus_tag="ACHKNB_RS05790"
                     /old_locus_tag="pgaptmp_001158"
     CDS             41826..42683
                     /gene="murI"
                     /locus_tag="ACHKNB_RS05790"
                     /old_locus_tag="pgaptmp_001158"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGU4194715.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9771 a        10698 c        11803 g        10637 t
ORIGIN      
        1 tgtaaaatat aatatatgtc atttgagaaa aaagagcaga atgaggtaaa aatctttggc
       61 tgtgtattca gtctggtaat catccaagga aagcttatca gaagggtgat gactccaatt
      121 agtagaacat atattttcga taaaagcacc gatgactttt tcatgaagtt ctacagaacg
      181 cgagaaacag atggttctgc gggccagacg tttgattcgg gttctcaggg tcagattgtt
      241 acgctcaatg tgctgagtaa aaatcttacc agtcagatgc ttctccttcg gtaactctct
      301 agcgtaacta ccccattcgt cagtggtcaa catgccgatg gtaaaagggt aagcagcgcc
      361 cgcaattcac gacaggtttc gtcagtccgg gggccaaaag tgtaggccag aacaccacca
      421 gttttcgtgt tgtacacgta ccagagccag tgctgtcgcg ctttactgcc gacaaagctc
      481 cattgctgac taagtttgca gatgagtgcc acatcagcat gagcgaccgg ggatgacgtt
      541 attcttcgtg gcgagagttt tttaaagttc ggatgactgt attaatgccg attttcagcg
      601 tacgggcagt atcgcgaacc ccggcaccgt tgaaagtcat ttcagcgatt tggtctttaa
      661 ctcccggttt acggggctcg taacagtaac tgagctgaaa tacccggtga cagtcacggc
      721 cgcggaatcg atcatgtctt tttgggttct gcccgtgacg gtaaacctga acggactggc
      781 aacgggggca atgaaggtta actgtgacca tgaaacaacc ttaaaagcga gtattatatc
      841 cttaattcaa ctaattggag gcatcaccgt ttaccggagc gacagcttgc ggaaaggtcg
      901 tgcaactaca aagcaacaaa cagactacgg cgggaattat ttttttcatc atccattacc
      961 acgaaaacag cggccactct ttggcaaccg tggtgagttg tggcgcgtcg aaaacggctt
     1021 gtggtgaaac taccgtttcc tcaagcggaa tatttttacc gtagcgttct ttgagcttcg
     1081 cctggaccag cgggttgctg agatcaaact ctttcaactt ctgctgttcg cccacgcgca
     1141 ttcccagcgc gttctggtaa accttccaca ccacttccga acagtactgg cgatcgtcgc
     1201 tccatgagaa gctgaaatca tacggtttac cgagataacg ttttgccgtt tgcgccagtt
     1261 tttgctgttg ttcaacactc agtccgcctt caacgcggcg aacgacgtat ttgccctttt
     1321 caccatgggc gatccactgc ttgagcgggg tgtatttcac cgggccgacc gcttcaaaaa
     1381 cgtagggctt tttgttgcgt atcaccagca taccggtgtg gctgtaatcg gaatgggttg
     1441 ccagttggat cgctttactt tgcgatgagc gagagatttg aaagatgatg tcgccagttt
     1501 gcggctgcca ggcaaaggca ggtacagaaa ccagcaggct gaggatcagc agcctgtttt
     1561 tcatagttaa acgtccatgt ataaaaagcg gtgggtcgca gacaacgtgc tcgttgttta
     1621 tgccggatgc ggcgtgaacg ccttatccgg cctacaagtt cgtgcaaatt caataaattg
     1681 caatatgacg taggcctgat aagcgtagcg catcaggcgg ttccactccg cgccgctctt
     1741 ttccgcttta gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg
     1801 ccgcttccgg gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt
     1861 gcccggtaaa ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca
     1921 ggttgttcac ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa
     1981 tggaaaccgt aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga
     2041 tatattcgcc gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac
     2101 cagaccacac agttaatgtg ggcactggcc gtaaacccgc atagtttacc gtacaggcgc
     2161 taccgttaca tcgggtaatc cacctgctgg cgtgataatg catataattt taacggctat
     2221 ttgggatttg ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat
     2281 taccacaatg tttactctgg tgcctgacat ttcaccaata aagcccaggg aacatcatca
     2341 catgacgcgt aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta
     2401 tggttgcgtt gtcccaccca tccatctttc cagtacctat aactttaccg gatttaatga
     2461 accgcgcgcg catgattact cgcgtcgtgg caacccaacg cgcgatgtgg ttcagcgagc
     2521 actggcagaa ctggaaggcg gtgctggtgc agtacttact aataccggca tgtccgcgat
     2581 tcacctggta acgaccgtct ttttgaaacc tggcgatctg ctggtagcgc cgcacgactg
     2641 ctacggcggt agctatcgcc tgttcgacag cctggcgaaa cgtggttgct atcgcgtgtt
     2701 gtttgttgat caaggcgatg aacaggcatt acgggcagcg ctggcagaaa aacccaaact
     2761 ggtactggta gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg
     2821 ccatctggca agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc
     2881 attacaaaat ccgctggcat taggtgccga tctggtgttg cattcatgca cgaaatatct
     2941 gaacggtcac tcagacgtgg tggccggcgt ggtgattgct aaagacccgg acgttgtcac
     3001 tgaacttgcc tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct
     3061 gctgctacgc gggttgcgaa cgctggtgcc gcgcatggag ttggcccagc gtaacgcgca
     3121 ggcgattgtg aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct
     3181 gccggaaaat caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt
     3241 gagttttgaa ctggatggcg atgagcagac gctgcgtcgt ttcctgagcg ggctgtcgtt
     3301 gtttacgctg gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat
     3361 gacacatgca ggcatggcac cagaagcgcg tgctgccgcc gggatctccg agacgctgct
     3421 gcgtatctcc accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt
     3481 ccgggctgca aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt
     3541 cagctgcata aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg
     3601 ggcattatgg cggagtactc tcagcctgac gatatgatgg tggtttccgc cgccggtagc
     3661 accactaacc agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat
     3721 caggttcaac aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgct
     3781 gaagaagccg atagcctcat tagcgctttt gtcagcgacc ttgagcgcct ggcggcgctg
     3841 ctcgacagcg gtattaacga cgcagtgtat gcggaagtgg tggggcacgg tgaagtgtgg
     3901 tcggcacgcc tgatgtctgc ggtacttaat caacaagggc tgccagcggc ctggcttgat
     3961 gcccgcgagt ttttacgcgc tgaacgcgcc gcacaaccgc aggttgatga agggctttct
     4021 tacccgttgc tgcaacagct gctggtgcaa catccgggca aacgtctggt ggtgaccgga
     4081 tttatcagcc gcaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat
     4141 tccgcgacac aaatcggtgc gctggcgggg gtttctcgcg taaccatctg gagcgacgtc
     4201 gccggggtat acagtgccga cccgcgtaaa gtgaaagatg cctgcctgct gccgttgctg
     4261 cgtctggatg aggccagcga actggcgcgc ctggcggctc ccgttcttca cgcccgtact
     4321 ttacagccgg tttctggcag cgaaatcgac ctgcaactgc gctgtagcta cacgccggat
     4381 caaggttcca cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc
     4441 agccacgatg atgtctgttt gattgagttt caggtgcccg ccagtcagga tttcaaactg
     4501 gcgcataaag agatcgacca aatcctgaaa cgcgcgcagg tacgcccgct ggcggttggc
     4561 gtacataacg atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg
     4621 ctgaaaatcc tcgacgaagc gggattacct ggcgaactgc gcctgcgtca ggggctggcg
     4681 ctggtggcga tggtcggtgc aggcgtcacc cgtaacccgc tgcattgcca ccgcttctgg
     4741 cagcaactga aaggccagcc ggtcgaattt acctggcagt ccgatgacgg catcagcctg
     4801 gtggcagtac tgcgcaccgg cccgaccgaa agcctgattc aggggctgca tcagtccgtc
     4861 ttccgcgcag aaaaacgcat cggcctggta ttgttcggta agggcaatat cggttcccgt
     4921 tggctggaac tgttcgcccg tgagcagagc acgctttcgg cacgtaccgg ctttgagttt
     4981 gtgctggcag gtgtggtgga cagccgccgc agcctgttga gctatgacgg gctggacgcc
     5041 agccgcgcgt tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc
     5101 ctgtggatgc gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag
     5161 cagcttgctg atcagtatct tgatttcgcc agccacggtt tccacgttat cagcgccaac
     5221 aaactggcgg gagccagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa
     5281 accgggcgtc actggctgta caatgccacc gtcggtgcgg gcttgccgat caaccacacc
     5341 gtgcgcgatc tgatcgacag cggcgatact attttgtcga tcagcgggat cttctccggc
     5401 acgctctcct ggctgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat
     5461 caggcgtggc agcagggctt aaccgagcct gatccgcgtg acgacctctc cggcaaagac
     5521 gtaatgcgca agctggtaat tctggcgcgt gaagcgggtt acaacattga accggaccag
     5581 gtacgtgtgg aatcgctggt gcctgctcat tgcgaaggcg gcagcatcga ccatttcttt
     5641 gaaaatggcg atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg
     5701 gggctggtgc tgcgctacgt ggcgcgtttc gatgccaacg gcaaagcgcg tgtgggcgtg
     5761 gaggcggtgc gtgaagacca tccgctggca tcactgctgc cgtgcgataa cgtctttgcc
     5821 atcgaaagcc gctggtatcg cgacaacccg ctggtgatcc gtgggcctgg cgcggggcgc
     5881 gacgtcaccg ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt
     5941 cagaaattta ataatgcccg gtactcatgt tttcgggttt atggtttcta atgaaatata
     6001 ttgaattatc ataggattag gccggattaa gcgtttacga cgaatccggc aagaagcaat
     6061 aagtacatgg ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt
     6121 cttaccatcc tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt
     6181 tcggttgacg cccttcggct tttccttcat ctttacatct ggacgtctaa acggatagat
     6241 gtgcacaaca caacatataa ctacaagcga ttgatgaggt aaggtatgag cttttttcac
     6301 gccagccagc gggatgccct gaatcagagc ctggcagaag tccaggggca aattaacgtt
     6361 tcgttcgagt ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc
     6421 gatcgcctta gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc
     6481 gagcgcgacc gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg
     6541 gcaccgcatc ttacttgcat tgatgcgacg cccgacgagc tgcgcaccat tgcacgcgac
     6601 tactggaata acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt
     6661 ggtaagccgg aaatgtatgc ttctgacctg gtgacgctgt taaaagaagt ggcagatttc
     6721 gatatctccg tggcggcgta tccggaggtt cacccggaag caaaaagcgc tcaggcggat
     6781 ttgctcaacc tgaaacgcaa agtcgatgcc ggagccaacc gcgcgattac tcagttcttc
     6841 tttgatgtcg aaagctacct gcgttttcgt gaccgctgcg tatcggcggg tattgatgtg
     6901 gaaattattc cgggaatttt gccggtctcg aactttaaac aagcgaagaa atttgccgat
     6961 atgaccaacg tacgtattcc ggcgtggatg gcacaaatgt ttgacgggct ggacgacgat
     7021 gccgaaaccc gcaaactggt gggagcgaat attgccatgg atatggtgaa gattttaagc
     7081 cgtgaaggag tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg
     7141 atttgccata cgctgggtgt tcgacctggt ttataaatag tgtggctttt gtgaaaatca
     7201 cacagtgatc acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgggaaa
     7261 ataaggttat cagccttgtt ttctccctca ttacttgaag gatatgaacc taaaaccctt
     7321 ttttataaag catttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt
     7381 gatctacatc tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat
     7441 tcaattataa cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac
     7501 attgatgagc acgtcagacg atattcataa caccacagcc actggcaaat gcccgttcca
     7561 tcagggcggt cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa
     7621 tcaactccgt gttgacctgt taaaccaaca ttctaatcgt tctaacccac tgggtgagga
     7681 ctttgactac cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa
     7741 agccctgctg acagaatctc agccgtggtg gcctgccgac tggggcagtt acgccggtct
     7801 gtttattcgt atggcctggc acggcgcggg tacttaccgt tcaatcgatg gacgcggcgg
     7861 cgcgggtcgt ggtcagcaac gttttgcacc gctgaactct tggccggata acgtaagcct
     7921 cgataaagcg cgtcgcctgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg
     7981 ggccgacctg tttatcctcg caggtaacgt ggcgctggaa aactccggct tccgtacctt
     8041 cggttttggt gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga
     8101 tgaaaaagcc tggctgactc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc
     8161 aaccgagatg ggcctgattt acgttaaccc ggaaggcccg gaccacagcg gcgaaccgct
     8221 ttctgcggca gcagctatcc gcgcgacctt cggcaacatg ggcatgaacg acgaagaaac
     8281 cgtggcgctg attgcgggtg gtcatacgct gggtaaaacc cacggtgccg gtccgacatc
     8341 aaatgtaggt cctgatccag aagctgcacc gattgaagaa caaggtttag gctgggcgag
     8401 cacttacggc agcggcgttg gcgcagatgc cattacctct ggtctggaag ttgtctggac
     8461 ccagacgccg acccagtgga gcaactattt cttcgagaac ctgttcaaat atgagtgggt
     8521 acagacccgc agtccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat
     8581 cccggatccg ttcgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac
     8641 gctgcgtttt gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc
     8701 gttcaacgaa gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa
     8761 atctcgttac atcggaccgg aagtaccgaa agaagatctg atctggcaag atccgctgcc
     8821 gcagccgatc tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga
     8881 ttctggtctg tctgttagtg agctggtatc ggtggcctgg gcatctgctt ctaccttccg
     8941 tggtggcgac aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga
     9001 ctgggatgtg aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa tccagaaaga
     9061 gtctggtaaa gcctcgctgg cggatatcat cgtgctggct ggtgtggttg gtgttgagaa
     9121 agccgcaagc gccgcaggtt tgagcattca tgtaccgttt gcgccgggtc gcgttgatgc
     9181 gcgtcaggat cagactgaca ttgagatgtt tgagctgctg gagccaattg ctgacggttt
     9241 ccgtaactat cgcgctcgtc tggacgtttc caccaccgag tcactgctga tcgacaaagc
     9301 acagcaactg acgctgaccg cgccggaaat gactgcgctg gtgggcggga tgcgtgtact
     9361 gggtgccaac ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag
     9421 caatgacttc ttcgtgaact tgctggatat gcgttacgag tggaaagcga ctgacgaatc
     9481 gaaagagctg ttcgaaggtc gtgaccgtga aaccggcgaa gtgaaataca cagccagccg
     9541 tgcggatctg gtatttggtt ctaactctgt cctgcgtgcg gtagcggaag tttacgccag
     9601 cagcgatgcc cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa
     9661 cctcgaccgt ttcgacctgc tgtaatctga ccttcttcag cgactgtctt tcaggcagtc
     9721 gctgaagttt ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat
     9781 gtctgccgca ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat
     9841 ctggagttat agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt
     9901 taccgcctta cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg
     9961 tcgcggaatg cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg
    10021 cgggatggtt ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat
    10081 cctaagctat accatgccgt tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg
    10141 cctgcgacgt gggcaatatt tcgcgatact gattgccgct ttcggcttat ttttggtgtt
    10201 gcagccgtgg caactcgatt tctcttcaat gaaaagtgcc atgctggcaa tcctctccgg
    10261 cgtcagttgg ggggcgagcg cgattgttgc taaacgccta tacacccgtc atccgcgcgt
    10321 ggatttatta tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt
    10381 cgctttactg gtgccgcaac gtgaaattga ctggcagccc accgtgttct gggcgctggc
    10441 ctacagtgcg attctggcga cggcgctggc gtggagctta tggttgtttg tattgaaaaa
    10501 cttgcctgcc agtattgcca gcttaagcac gctggccgtt cccgtttgcg gcgtactctt
    10561 ttcctggtgg ctgctcggtg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat
    10621 tgtgctggca ctggcgctgg tgagccgtaa gaaaaaagaa gctgtcagtg taaaacgcat
    10681 ctgaattttt tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac
    10741 catccgccag ctaaacagca catcttcttc ctgcgcgcct gcgccaatgt tatgtatcac
    10801 cagcggcgta ccgtcgcggg cgaagccatc tgacaccacc ccaatatgtg ccagtccgtt
    10861 atccagtcgc caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg
    10921 gcgtgtttta tcgtggcggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat
    10981 gttgctgtcc gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat
    11041 atcttcgtgc accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac
    11101 atcggaacat accccgcgtt cttgcgggac atcaccgcca ggataggtaa gctgcatata
    11161 cgccggatcg taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag
    11221 gttggtattg gcctggattt gtaccacggt tggtggaacg gcgggagatt ttaaggagtg
    11281 gctggtaaat gccgttagca ggctgagcag cgccagtgaa gctttcatct ggttcgtccg
    11341 tgaaaataag agtcacagat tcgaccttcc gggctaacga ttgttctgtg gagaaaatgt
    11401 ccagaagcgg ataaaaaact cctgaacaac gtgggagttt cagattactg gcacagagtg
    11461 ccggatgcgg agtgaacgcc ttatccggcc tacaaaagca cgcatattca atatattgca
    11521 gagatttcgt aggcttgata aacgtagcgc atcaggcaat tttgcgttca aactcccgga
    11581 caagccggga gtttggagta ggttattccc actcttgcag gaaacgctga ccgtactggt
    11641 cggctaccag cagagcggcg taaacctgat ctggcgtcgc gccgccaggc atgttgtgaa
    11701 tggtttcacc ttctgcacat gccgcttctg ccacaattcg cattttcgcc gggacatctt
    11761 ctttaatatc cagttgagcg agagttattg gcaaacctac cgcatggcta agtgcagcta
    11821 cggtttcgat ttcctcaacc ggtgcgtttt ccagaaccag ctgcgtcagc gtaccaaatg
    11881 ccactttttc accgtgataa tagtgatgcg cgtccgggat agcggtcagg ccgttatgca
    11941 ccgcgtgagc cgccgccagc ccgccgcttt caaaaccaac gccgctcaaa taggtgttcg
    12001 cttcaatcac gtgctccagc gccggggtta ctacatgctg ttcggcggca agcatcgctt
    12061 tttcgccttc ttccagcagg gtgttgtagc acagttcagc cagtgccagc gccgcctggg
    12121 tgcacttgcc gcccgccatg gtggtcgcgc cgctacgaga gcaagcacgc gcttcaaacc
    12181 aggttgccag cgcatcgccg atacccgctg ctaacagacg tgcaggtgcg cccgcgacga
    12241 ttttggtgtc gacaatgacc atattcgggt tatttggcaa cagcagatag cggtcaaact
    12301 caccctcatc ggtgtagata acagacaatg cgctgcacgg tgcatcggta gaggcgatag
    12361 tcggtgcgat cgctaccgga acacccatga aatgtgccag tgctttggca gtatcgaggg
    12421 tttttccgcc accgataccg agaattgcgc cacactgcgc agtctccgcg atgccacgca
    12481 gacggtcgat ctcattttgc gaacattcac cgccaaacgg cgcaatttct actaccagtc
    12541 cagcatcttt aaagcttttc tcgacagtgg attgagcaaa acctaaaaca aatttgtcac
    12601 ccaccactaa ccagcgttct gccagcggct tcaggtattc gcccagacga ttaatcacat
    12661 cagcgccctg gatgtattta cccggtgatt gaataatgcg gtccataatt gctcctttag
    12721 agatgagtag tgccaaatgc ggcattccag tcgtgttcga acttctctat agctgactct
    12781 accgcagggg tgttgagcat ttgttgcgct acatctaagg gcagggtgat ggattcacat
    12841 cctgccagta aacagtccag cgcctgacgc ggcgttttaa agctggccgc cagcaccatg
    12901 ctttctggtg cgtgcatttc taacagcgct tgcagctcct gaaccgtacg aatgccgtcc
    12961 ccgccctggg catctacgcg gttaacatac ggagcaacgt attttgcccc tgccagcgcg
    13021 gccagtaacc cttgtgcagc actgtacact gcggtgccaa gcgtagtaat accctctttt
    13081 ttcagcatct taatggcggc cagaccttcg gaagtcaccg ggattttcac cacaatgccc
    13141 ggaatggcgt cgcgcaggcg cttcgcttct tccaccatac cctgcgcgtc acgactcata
    13201 gtctgagcaa acagaatgcc ctcatcacca atcgcttttt gcagacgcgg cagaacttcc
    13261 catatggact ccttgctggc agcgataatg ctcgggttag ttgtcacccc ggcaatgggg
    13321 aatatgcgtg ccagacgttc gacttctgcg acgttagcgg tgtccagata cagttccatg
    13381 atgtttcctt ttacagttcc agttcatgtt gcagcaggct ggcgatagcg tcggcagagg
    13441 cggcgttaac cagcgcgtta cggaattctt cgtgcatgat gcggcgagcg aggcgcgaga
    13501 aaatgcgcat gtgctgatcg cccgcagcgt gtttgttcag ggttaacata atgatgaatt
    13561 gcgcttcatc atcgccccag cgcaccggcg cttgcagacg cgccacgctg atggtggatt
    13621 gctcaatgtg ttctgatttg ctgtgtggaa tggcaaaact aaagcccaga ccggtagaga
    13681 aaacggcctc gcgcgcccat aagtcggctt ccagtttacg cggatagcgg cagcggcccg
    13741 ccagcagcag gttatcggtc atgcctttga gcacttcttc tttgctgcgc cagtcacttt
    13801 ccagcgtgat gcactcggcg gtgaccagcg gtgcgtcctg ttgggtcatg cggaattgcg
    13861 ccagcaggtg ttccacttcc agcgaagtac ggcaggccat tgcctggttg agcaacttgc
    13921 ggcactcacg gctatcaagt tgcgccatcc gcgctttcgc cgccggaatt gatggtgcgc
    13981 tcatgctgag ttcatccagc cctaagccga ccagcaacgg cagcacggaa cctttcgctc
    14041 ccagctcacc gcacagacca atccatttgc cctggcgatg caccgcctgc acggcgtaat
    14101 cgagcgcccg caagaatgcc ggattcaggc tgttgtagtg acgagtaacc ttagcgttat
    14161 cgcgatccac tgccagcaga tattgcgtca ggtcattact accaatacta aagaaatcaa
    14221 tctcttcgca gcattgatcg atgatgaaca tcaccgacgg tacttccagc ataatgccaa
    14281 gctggatctt ctcatcaaac ggaatgtgtt cgttacgcag ttgctgtttg gcttccgcca
    14341 gtttttcttt cacccataag atctcttcca ttgaggagat catcgggatc atgattttca
    14401 ggctgccgtg agcggaggcg cgcaggatcg accgtagttg tgtggtgaac agcgaggcgt
    14461 attcttcata aatacgcaca gcgcgataac cgaggaacgg gttagcttct gccggaatgt
    14521 tcagataatc aacgggtttg tcgccgccaa tgtccatagt gcgcacaata atgctgcgtc
    14581 cgttggcaga ctccagcgcc tgacaaaaaa tgttgtacag ctcgctttcg cccggtgcac
    14641 tggtgcgatc catatagagc atttcagtgc ggaacaaacc aacgccttcc gcaccattgc
    14701 cgaatgcggc ctgcgcttcc acggagtgag cgatgttagc ggcaatttca atgcggatac
    14761 cgtcagcggt acgggcttgt tgggtcagcc agacacgctg ttgctcacgc agggcgtcct
    14821 gtacgcgggc ttcttgctga taataacgag ctacggcttc tcctggctca accacaatcg
    14881 ccccagcgtt gccgtcgata taaatcgttt gatgctgcca cggagtaagg gcatcaatat
    14941 ctacaccaac cagtgtcgga atgttgaacg aacgggcaag aattacggta tgtgaggtgg
    15001 tgccgccact tttcagtaac agacctttga ggtgattttt atccagttcg aggaactggc
    15061 tgggagtcag ttcatcagcc atacaaatgg cgggctgcgt cagtttgccc ggtgccggga
    15121 agcgttgctc accgtagatt tgctggagta actggaagca gacgtcgcgt acgtccaggg
    15181 cacgttcttg caggtagctg ctgctggaac gggcgaactc ttcgcaaaag tgattcgcac
    15241 tggtgacaat cgcttcggcg cagcttaatc cggcgctgac gcctgccagt aaatgttcgc
    15301 gcagggaagt atcgccagcc agggatcggt gcgcttccag aatcgcgctg gtggcaccgt
    15361 cgctatccag cagacgaaac tcaatgtttt tcagtaccag cgtcaggccg ttttccagtg
    15421 cggattgctc ggcgtcaacg cctttggctg cgggaagatt acccagcgca ttgagatcta
    15481 aagaagagat cggcgtcaga atgccgcctg cactaccgct gcacacggtg cgggcgcgga
    15541 taatttgcgg attcagattg gtcagtgaaa ccggcagtgg ttccagttcg tcagatttaa
    15601 cttccgccag cggcgcatcg cagtggggga attcatcgcg cagccattgg cttaaacgct
    15661 ggtgcgcttc ctgttcgtcg gccccggaaa tcagtaactg gcagttatcg cccgccagcg
    15721 tatcggtgcc aatcagcgca agggcgcttt tggcgttgcc cttgcggtca gtgcgcaggt
    15781 tatgccactc aatttgtgat gaaaaagtat tacacagcgt ttcaacgtgg cttgccggac
    15841 gtgcatgtac gccgttaggt agctcacaaa taaattccac aatcagggcc attgcctctc
    15901 tcccggtacg gttttctgtt tacaggataa gagcgcacgg caacggcctg ccatgtgaca
    15961 aatctgccaa aagctggaca aatgtaatgt aaccgtcaat ttgcgacgcg tctcacaaga
    16021 cgctgttttg cggcatgctt ccggtttatc gcaagttatg aggcggatcg catttttgta
    16081 ctgatattac aaaagtccag taaatggcct ttttatccac tgtttgctcc gcctgcgatt
    16141 gcctattgtt tcctccatca aaaatatgga catgggcttt cgcaagcccg ggagcagctt
    16201 atgaatgagt tggtgcagat cctgaaaaat acccgtcagc atttaatgac gggcgtttca
    16261 cacatgattc ccttcgtggt atcgggcggt attttgctgg cggtttccgt catgttgtat
    16321 ggcaaaggcg cagtgccgga tgccgtagcc gatccaaatc tgaaaaaact gtttgatatc
    16381 ggcgttgcgg gcttgacgct gatggtgcct ttcctcgcag cgtacatcgg ttattccatt
    16441 gcagagcgtt ctgcgctggc tccgtgcgct atcggtgcct gggttggtaa cagctttggt
    16501 gcgggcttct ttggtgcact gatcgccggg attatcggcg gcatcgtggt gcattacctg
    16561 aagaaaattc cggtgcataa agttctgcgc tcggtgatgc ctatcttcat tattcctatc
    16621 gtcggcacac tgattaccgc aggcatcatg atgtggggct tgggcgagcc tgtaggggcg
    16681 ttgaccaaca gcctgactca gtggcttcag gggatgcagc agggcagcat tgttatgctg
    16741 gcggtgatca tgggtctgat gctggcgttc gatatgggcg gtccggttaa caaagtggcc
    16801 tatgccttca tgctgatttg cgttgctcag ggtgtttata ccgtggtggc catcgctgcc
    16861 gtgggtattt gtatcccgcc gctggggatg gggctggcga cgctgattgg tcgtaaaaat
    16921 ttctccgcag aagagcgcga aaccggcaaa gcggcactgg tgatgggctg tgttggcgtt
    16981 actgaagggg cgattccttt cgccgctgcc gatccgctgc gtgtcattcc ttccatcatg
    17041 gtcggttcag tttgtggtgc agtaactgcg gcgctggtcg gtgcgcagtg ctatgcaggc
    17101 tggggtggtc tgattgtgct gccggtggtt gaaggcaagc tgggttatat cgcagcagtg
    17161 gctgtcggag cagtggtgac ggctgtttgt gtgaacgtgc tgaaaagtct ggcgcgtaaa
    17221 aatgggtctt cgactgatga aaaagaagac gacctggatt tggattttga aattaattaa
    17281 ttgaggaacg agccatgacg aaaattattg cagtaaccgc atgtccttca ggtgttgccc
    17341 atacctatat ggcagcagag gcgctggaaa gtgccgcgaa agcgaaaggc tgggaagtga
    17401 aagtagaaac ccaggggtca attggtctgg aaaatgaact gactgcggaa gatgtggcga
    17461 gcgctgatat ggtgattctg accaaagata tcggcatcaa gtttgaagag cgttttgctg
    17521 gcaagaccat cgtgcgcgtc aacatcagcg atgcagtaaa acgtgctgac gccattatga
    17581 gcaaaattga agctcacctg gcgcaaaccg cttaagttct tctcccgctc gcaagggcgg
    17641 gttcgctttc ccacaggagt tcctcatgac gaatcgtatc tctcgcctca aaactgcact
    17701 gtttgccaat acccgtgaaa tctcgctgga gcgggcgctg ctttataccg ccagccatcg
    17761 gcaaaccgaa ggcgaaccgg tgatattgcg ccgggcgaaa gcaacagcgt atatccttga
    17821 acatgttgaa atttcgattc gtgatgaaga actgattgcc ggtaaccgca ccgtaaaacc
    17881 gcgcgctggg attatgtcgc cggaaatgga cccttactgg ctgctgaaag agctggatca
    17941 attcccgacg cgtccgcagg accgctttgc tatcagtgaa gaagataaac gtatctaccg
    18001 tgaagagttg ttcccgtact gggaaaaacg ttcgatgaaa gatttcatca acgggcagat
    18061 gacagatgaa gtaaaagccg cgaccagcac gcagcttttc agcatcaacc agacagataa
    18121 aggccagggg cacattatta ttgattaccc acgcctgctg aatcacgggc tgggggagct
    18181 ggtggcgcag atgcagcaac attgtcagca acagccggag aatcactttt atcaggccgc
    18241 gctgttactg ctggaagcct cgcagaaaca cattctgcgt tacgctgaac tggcggaaac
    18301 aatggcggca aactgcacag atgcccagcg tcgcgaagaa ctgctgacta ttgcggagat
    18361 ctcccgccat aacgccgaac ataagccgca gacgttctgg caggcgtgcc agttattctg
    18421 gtacatgaac atcattctgc aatacgaatc caacgccagt tcgctatcgt tggggcgctt
    18481 cgaccagtat atgttgccgt tctatcaggc atctttaacc cagggcgaag atccggcgtt
    18541 cctgaaagaa ctactcgaat ctttatgggt gaaatgcaac gacatcgtgc tgttgcgctc
    18601 caccagcagc gcgcgttatt tcgcaggttt cccgaccggc tataccgcac tgctcggcgg
    18661 gttaaccgag aacggacgta gcgcggtgaa cgtgctttcg ttcctttgcc ttgacgctta
    18721 tcaaagcgtg caattaccgc aaccgaacct cggcgtgcgc actaacgcct tgatcgacac
    18781 gccgttcctg atgaaaaccg ccgaaaccat tcgcctcggc accggtattc cgcaaatctt
    18841 taacgatgaa gtggtggtac cagcgttcct caaccgtggc gtttcgctgg aagatgcgcg
    18901 cgactattcc gtagtgggct gtgtggaatt atctattccc ggcagaacct acggcttgca
    18961 tgacatcgcg atgtttaatc tgctgaaagt gatggaaatc tgcctgcatg aaaatgaagg
    19021 caatgctgcg ctgacttatg aaggtttact ggagcagatc cgcgccaaga tcagccacta
    19081 catcaccctg atggttgagg gcagcaatat ttgtgatatc ggccatcgcg actgggcacc
    19141 tgtaccgctg ctctcgtctt ttatcagcga ttgtctggaa aaaggtcgcg atattaccga
    19201 tggcggcgcg cgttataact tctccggcgt acaggggatc ggtatcgcca acctgagcga
    19261 ttctctccat gcgttgaaag ggatggtttt tgagcaacag cgtttaagtt ttgacgaatt
    19321 gctgtcggta ttaaaagcca actttgcaac gccagaaggc gaaaaagtcc gtgctcgctt
    19381 aattaaccgc ttcgagaaat acggtaacga tatcgacgag gtggataaca tcagcgccga
    19441 actgttacgc cactactgca aagaagtgga aaaataccag aacccgcgcg gcggttactt
    19501 cacgccggga tcgtataccg tttctgctca cgtcccgttg ggatcggtgg ttggcgcgac
    19561 gccagacggt cgttttgccg gggaacaact ggcagacggc ggcttgtcac ctatgctggg
    19621 ccaggacgca caagggccaa cagcggtact gaagtcagtc agtaagctcg ataatacgct
    19681 gctgtctaac ggtacgttgc tgaacgtgaa attcactccg gcgaccctgg aaggtgaagc
    19741 gggattacgc aaactggccg acttcttacg ggcgtttacc cagcttaagt tgcagcatat
    19801 tcagtttaac gtggtgaacg ccgacacgtt gcgggaagcg caacagcgcc cacaagatta
    19861 tgccgggctg gtggtgcgcg ttgccggata cagcgccttc tttgtcgaac tgtcgaagga
    19921 gatccaggat gacatcatcc gccggacagc gcatcagctg taacgttgtg gaaacgcgcc
    19981 gcaatgatgt ggcgcgcatt ttcaacattc agcgttattc actgaatgac ggcgagggca
    20041 ttcgtacggt ggtctttttt aaaggctgtc cgcatctttg cccgtggtgt gctaatccgg
    20101 agtcgatctc cggcaaaatc cagacggtac gcagagaggc gaaatgtctg cactgtgcga
    20161 aatgtttgcg tgatgcggat gaatgcccct ccggtgcgtt tgaacggatt ggtcgcgata
    20221 tcagccttga cgctctggaa cgggaagtga tgaaagatga cattttcttt cgcacgtccg
    20281 gcggcggcgt cacgctttct ggcggcgaag tgttaatgca ggcggagttt gctacccgtt
    20341 ttttacagcg actgcggctg tggggtgtct catgtgccat tgaaactgcc ggagacgcgc
    20401 cggccagcaa gctgttacca ctggcgaaat tgtgcgatga agtgttgttc gatttaaaaa
    20461 ttatggatgc gactcaggcg cgggatgtgg tgaatatgaa cctgccacgc gtgctggaga
    20521 atctgcgttt gctggtgagt gagggcgtca acgtgatccc gcgtttaccg ctgatccctg
    20581 gtttcacgct cagccgggag aatatgcagc aggcgctgga cgtgctgatc ccgctgaata
    20641 tcaggcagat ccatctgtta ccgtttcatc agtacggcga accgaaatac cgcctgctgg
    20701 ggaaaacatg gtcgatgaaa gaggtgcctg cgccgtcgtc agccgatgtg acaacgatgc
    20761 gcgaaatggc agaacgggcc ggatttcagg ttaccgtggg aggttaaaat ggcatacctg
    20821 gtggcagtaa ccgcctgcgt aagcggcgtg gcgcatacat atatggcggc ggaacggctg
    20881 gaaaagttgt gccagttaga gaagtggggc gtcagcattg aaactcaggg cgcgttggga
    20941 acggagaatc gtttagcgga cgaggatatc cgtcgggcgg atgttgctct gttgattacg
    21001 gatatcgagc ttgccggtgc cgagcgattt gaacattgcc gctatgtgca atgcagcatc
    21061 tacgcattcc tgcgtgagcc gcagcgggta atgagcgcgg tgcgcaaagt gctttctgca
    21121 ccgcagcaaa cccatcttat tctggagtag tcggtttttc tgtcagttgg ctgtggtact
    21181 gccggcgata ttccgacggc gagcgttcgg tgtttttacg aaacagacgg cagaagtagt
    21241 tgctgtcgac aaagccgcag gcgtgcgcca cttcttttac cttcaggtca tagcctttca
    21301 ataacgtctt agcgtgctcc agtcgcgtgt gattcaggta ttcgttaaaa ccaatggccc
    21361 ccgttttttg aaacaggtgc gagagatagt ttggtgaaat atagaatgcc tgtgcgacag
    21421 attcgcgggt aagcgcggag gcatagcgtt cgtcgatata atcgcgaata gcttcaaata
    21481 gtgcctggct gcgtgaggcg gtctggattt ggctgccgag caaatcgcgg cagtggctaa
    21541 gcaggctggc tacgataagc cttgccgttt gctgctcctg cggctgcatt tgcatttcat
    21601 tgagcgtttg tagcagaaaa gaacctatac gtgggccgcg tcgggcgacg tgttgcttcg
    21661 ccagattttg atattgtttg ccatcccatt gcacgacgct gaagccgagt tgctgtttgc
    21721 caaacagcac gctaaaggtg gtagcgggtg ttttccattg tgggaaattc cagccaccag
    21781 cggcaacgta cagcacatcg ccgggaacta acgttgctcc agcgccggta tcaacaaact
    21841 caccttccag cacaatttcc agccgtggaa aatcgacctg atacgcaaga tcgggcaccg
    21901 ggccgttact gctggcaaag taaatctggc gcagggacag cgggccgttg atcaggcggg
    21961 agagcagata gctgacgtcg tgatacatgt tattccctta ggtatgtaga cgggaaaaca
    22021 gtaactggca ttattaaaaa aggccacctg aaaagtgtgg cctgagggga gttcgatgta
    22081 aacattgcgg cagtatcgca tccggcaaat agcgccagac gcgatacaaa ggtcatttac
    22141 tgattaccta cctggtcgcc atacggcaga gtgtcgtaat cgatcagcgc gtttttcttg
    22201 tacgggttac caatccagcg ggtagtttct ttgaactgcg gattcaccac tgaacgggtt
    22261 ggatcgtaac cgtcgtaaga taagcccgcc aaatctgacc aggtgtggat cagttccgcc
    22321 aggctgtatt tacgatcgac atcctgtgag aaatcacggg gatgagtcgc ttgccatttt
    22381 tccgacgtcc acagcaggaa cggaatggtg tacatgtggc gcgtcgggtt gtcttcgtta
    22441 cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc agagaagtaa
    22501 accaggaatc cgttcgggtc tgccgcttta aagtctttaa tcaggctggc aaccacatga
    22561 tcgttataca ggttagcgtt gtcataatcg ttatatgatt ccagctcttc tgcgcttaat
    22621 cctggcggaa catgatcggt attgccatca aacttgccct gattttccgg gtagcggtat
    22681 ttgtatttga tatgcgtacc cagcagatga acgatgatca gtttcttcgg cgcagggtca
    22741 ttcagcacat cctggaacgg cttcagcacg ttggtgtcgt attcacgcgc actctgcgta
    22801 cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg aaaataccgt cagcatggta
    22861 ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg ttttataacc cgcctgtttc
    22921 atcatgttca tcagcgacgg ctgcgtcaga tacagatccg ggttcttttc attggcaaag
    22981 gtcagcgcct gttgcaggat ttcaatggtg tacggacgag acgtaactac gttattaaac
    23041 acggtcagat tcggatcggt tttatgcagc gcatccagct ccggcgtggt ttcacgcgga
    23101 taaccgtaca gactcatgcg tccgcgctgg gtcgactcgc caatcaccag cactaaagtg
    23161 cgcggttcgt tacccgattc atctttgaaa ttagccagtg gcggcaaggc attattttca
    23221 ttcagtaact ttgttagcga gtttagttgc tgacgatact gataatagcc ggtcaggaat
    23281 tgccacggtg cggcaggctc catacgcgag gccaggttat ccaacgtttt ctcaaacggc
    23341 ttgtttttga taaacgtatt catggcgatc ggatgcagaa tcaagccata aagcagggca
    23401 aaagagacaa cataacgcca cggctttgga atatagaccg ggcgcaggcg tgtccacagc
    23461 agaactgcca ccgccgtata ggccagcgcg ataagcacaa ttttcaggct gaaatactgg
    23521 cttaaatact cgctggcttc gttggtgttg gtttcgaaca tcacaaacag aacgctctgc
    23581 gagaactcct gcccgtagat gacgtagtag catagcgccg ccagagaggc cgcccatagc
    23641 accacgccga ttactgcggc aataatttta atccgcttcg gaaagaggaa taccgggatc
    23701 aaccacagcg agctgaataa cagcgagtcg cgaatgccgt tggtgccact ataaccactg
    23761 atgtaaataa tggcctgtag cagagtagag aaaaaccaaa agtagagcag tgcccaaccc
    23821 agggctttcc agctaaaaag gggtttagcc tggacttctg tggaatgcat agtaagaacc
    23881 tgtcttgaaa aaaatatcgc cgaatgtaac gacaattcct taaggatatc tgaaggtata
    23941 ttcagaattt gaataaaatg cagacagaaa tatattgaaa acgagggtgt tagaacagaa
    24001 gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc agaagaggaa gattagccgg
    24061 tattacgcat acctgccgca atcccggcaa tagtgaccat taacgcttgt tcgacgcgag
    24121 gatccggttc ctggccttct ttttctgcct ggcgggagcg gtgcagtaac tcggcctgca
    24181 atacgttcag cgggtcggtg taaatattcc gtagctgaat agactctgca atccacggca
    24241 gatcggccat tagatgggaa tcgttggcaa tcgccagcac cactttgatg tcttcttctt
    24301 gcaggttgcg taactcttta cctaacggcc acagtgcttt gtctaccagg cgttggtcat
    24361 agtattccgc cagccacagg tctgctttgg cgaagaccat ctccagcatg ccgagacgcg
    24421 tcgagaagaa tggccaatcg cggcacatgg cttccagctc gctctgtttg ccatcttcga
    24481 ccactttttg cagcgccgta cctgcaccca gccaggcggg gagcatcagg cggttttgcg
    24541 tccaggcaaa aatccacggt atggcgcgca gcgactcgac gccgccggtt gggcgacgtt
    24601 tcgccggacg tgaacccaac ggcagtttgc ccaattcttg ttccggcgta gcggagcgga
    24661 agtaaggcac aaaatctttg ttttcacgta cgtagccacg gtagagatcg caggagatga
    24721 ctgacagttc atccataatg cgacgccagc tctctttcgg ctcaggtggt ggcagcaggt
    24781 tggcttccag aatcgccccg gtgtacagcg acaggctgct gacggtgatt tctggcagac
    24841 catatttaaa gcggatcatc tcgccctgtt cagtcacgcg caggccacct ttcaggcttc
    24901 ctggcggttg tgacagcagc gccgcatgag caggtgcgcc gccgcgacca atggaaccgc
    24961 cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc gcaggttttg attaatgcat
    25021 cctgtgcctg atattgcgcc caggaagctg ccatcactcc cgcatctttt gctgagtcgg
    25081 aatagccaat catcaccatc tgtttgccct gaatcaggcc acgataccag tcaatattga
    25141 gcagctgggt catgacatcg ttggcgttgt tcagatcatc gagggtttca aacagcggag
    25201 caaccggcat cgcaaacccg atacccgctt ctttcagcag caggtggaca gccagtacgt
    25261 cggacggcgt tttcgccatc gagatcacgt aggcggcaat ggagccttgc ggcgcttcgg
    25321 caatcacctg gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc cagttgcgcg
    25381 gcagaagcgg acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg tcggcctctg
    25441 accagctttc gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc gcttcggtat
    25501 gacgcgtgct ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa catttcacgc
    25561 ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc catgccacac gcctgaagtg
    25621 actggtagca agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc aggccttctg
    25681 gttttggcag ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc gccatcaggc
    25741 gagaacgcag gtttttcatc agatagcgat acggttctgc ggcaccttct tcgccaacca
    25801 gcgccagcag ttcaggggtc gcttcaacca tcgacagttc agaaaccagc acctgaatat
    25861 ctttcaggaa caaatcggtg gctttccagc ggctgagtag caggacgtgg cgggtgatat
    25921 cggcagtgac gttcgggttg ccgtcgcggt cgccgcccat ccacgaagta aaacggaccg
    25981 gaacaaattc gacgggcagt ttgtagccga ggttctcttc cagttgttcg ttcagttcgc
    26041 gcaggtaatt tggtacgcct tgccacaggc tgttttccac tacggcaaag ccccatttgg
    26101 cttcatctac cgggcttgga cgcagcttac ggatttcatc ggtatgccat gactgggcga
    26161 ttaactggcg caggcggcgc atcagctggt tgtgttcgta gtcagcgata tctttgttat
    26221 cgagctgttt taaacaggcg ttcacttcca ccattttgtg gatcagtgta cgacgggtaa
    26281 tttcggttgg gtgagccgtg aggaccagtt ccagcgacag cgattccact gcttttttga
    26341 tggtgtcttc gctcagttcc ggctggtttt tcagtttacg cagggtgcgg gcgatcactt
    26401 ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg gcggtgttgg
    26461 ccaggttcag gaactgacta aacgcacgcg caacgggcag cagctcgtcg ttcgacaaat
    26521 tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt gaagatttcg
    26581 acaacttacg gatagtttct acgcgttcaa gaatgtgttc tcccaacgca tccttgatgg
    26641 tttctcccag cactttgccg agcatactga cattactacg caatgcggaa tattgttcgt
    26701 tcatattacc ccagacaccc catcttatcg tttgatagcc ctgtatcctt cacgtcgcat
    26761 tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt ataaagccac gtaaaagcgg
    26821 tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa tttacgccat
    26881 taaattcacg acgctttaaa taagcgtaac ttatggaaat gttaaaaaat cgccatacat
    26941 aacaccaaag gtgtaggtcg gataagatgc gcaagcatcg catccgacat tattgcggca
    27001 caggagtttg gcaacggtgc cggatgcggc gcgagcgcct tatccggcct acgttttaat
    27061 gccagcaaaa atggtgaatt acctgggtta tcagttcgcg ggtgggcttg ataaaccgtg
    27121 tttccagata ttcatcaggt tgatgagcct gattaattga gccaggcccc aacaccagcg
    27181 tcgggcataa cgtttgaata aacggcgctt cggtacagta gttcaccact tcggtctttg
    27241 ctccgagcaa tttctcaact acttcaacca gttgatgatt cggtgggcat tcatagccag
    27301 ggatcggcgg atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg ctcaccggag
    27361 ccaatgcatc gttgagcaaa ccattaagtt cattgagtgt catgccaggc agcggacgaa
    27421 tatccatatg caactcacag caagcgcaaa tacggttaga agcgtcgcca ccgtgaatat
    27481 gcccgaggtt gagcgtaggg tatggcacgg taaacgcttc gtagtgataa cgttctttca
    27541 ggttatcgcg caattgcaaa atatgcccga tggcgtcgtg cattagttcg atagcgttaa
    27601 ctccgcgtgc tggatcgctg gagtgccccg actggccctg aatacggatg gcgttagaga
    27661 tatgaccttt atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg atggcgcaat
    27721 ccgggcgcag ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg gtttcttcat
    27781 cagcagtcgc cagaatgtag agcggttttt tcagtttcgt gacgtcgaca tcgcgtagcg
    27841 catcaaggat aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg taaagcttgc
    27901 cgtcatgctc cgtcagtgta aacggatcgc gcgtccagcg accgtcatca aatggcaccg
    27961 tatcggtatg ccccgccagc aacaagccgc cagccccctg tccgatactt gccagcatat
    28021 tgaatttgtt gcgagttcct ggcacaggct gcacttccac attgaagccc aaatctttaa
    28081 accagtccgc cagcagagtg attaaatctg cattgctttg atcgagtgcc tcttccgtgg
    28141 cgcttattga aggtgtggca atcagagcac ggtaaatctc gataaatggc ggtaatttgt
    28201 ttttcattgt tgacacacct ctggtcatga tagtatcaat attcatgcag tatttatgaa
    28261 taaaaataca ctaacgttga gcgtaataaa acccaccagc cgtaaggtga atgttttacg
    28321 tttaacctgg caaccagaca taagaaggtg aatagccccg atgttgaata cgctgattgt
    28381 gggtgccagc ggctacgctg gcgcagagct agtgacctat gtaaatcgcc atccgcatat
    28441 gaacataacc gctttgactg tttcagcgca aagcaatgat gcgggaaagt taatctccga
    28501 tttgcatccg cagctaaaag gcatcgttga tctgccgttg cagccgatgt cggatatcag
    28561 cgagtttagc ccaggggtgg acgtagtgtt tctcgccacc gcccatgaag ttagccacga
    28621 tttagcgccg cagtttcttg aagcgggctg cgtggtgttc gacctttccg gcgcgtttcg
    28681 tgttaacgac gccaccttct atgaaaaata ttacggcttt acccatcaat acccggaact
    28741 gctggaacag gcagcctacg gtctggcgga gtggtgcggt aataaattaa aagaagcgaa
    28801 tttgattgcg gtgccaggct gttatccgac ggcggcacag ctggcgctga aaccgttgat
    28861 tgatgccgat cttcttgacc tcaatcagtg gccggtgatc aacgccacca gcggcgtgag
    28921 cggtgcagga cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc tgcaaccgta
    28981 tggcgtcttt actcatcgcc atcaaccaga gatcgccaca cacctcggtg ctgacgttat
    29041 cttcactccg catctgggca atttcccgcg cggcattctc gaaaccatta cctgccgcct
    29101 gaaatcgggt gtgacccagg cgcaagtcgc gcaagtgtta cagcaggcgt atgcccataa
    29161 accgctggtg cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg ttgggctgcc
    29221 attttgcgat atcgggtttg ccgttcaggg cgagcatctg attattgtgg cgaccgaaga
    29281 caacttactg aaaggcgcgg cggcacaagc ggtacagtgc gccaatattc gtttcggcta
    29341 tgcggaaacg cagtctctta tttaagggtg caatgatgaa tccattaatt atcaaactgg
    29401 gcggcgtact gctggatagt gaagaggcgc tggaacgtct gtttagcgca ctggtgaatt
    29461 atcgtgagtc acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc gtggtggatg
    29521 agctgatgaa agggctgaat ctgccggtga aaaagaaaaa cggcctgcgg gtgacgcctg
    29581 ctgatcagat agacattatc accggagcac tggcgggaac ggcaaataaa accctgttgg
    29641 catgggcgaa gaaacatcag attgcggccg taggtttgtt tctcggtgac ggcgacagcg
    29701 tcaaagtgac ccagcttgat gaagagttag gtcatgttgg actggcgcag ccaggttcgc
    29761 ctaagcttat caactccttg ctggagaacg gttatctgcc ggtggtcagc tccattggcg
    29821 taacagacga agggcaactg atgaacgtca atgccgacca ggcggcaacg gcgctggcgg
    29881 caacgctggg cgcggatctg attttgctct ccgacgtcag cggcattctc gacggcaaag
    29941 ggcaacgcat tgccgaaatg accgccgcga aagcagaaca actgattgag cagggcatta
    30001 ttactgacgg catgatagtg aaagtgaacg cggcgctgga tgcggcccgc acgctgggcc
    30061 gtccggtaga tatcgcctcc tggcgtcatg cggagcagct tccggcactg tttaacggta
    30121 tgccgatggg tacgcggatt ttagcttaag ttttgttggc cggaggcgca gctttccggc
    30181 attgaatttc aaaataagga aacagagtta tggcactttg gggcgggcgt tttacccagg
    30241 cagcagatca acggttcaaa caattcaacg actcactgcg ctttgattac cgtctggcgg
    30301 agcaggatat tgttggctct gtggcctggt ccaaagccct ggtcacggta ggcgtgttaa
    30361 ccgcagaaga gcaggcgcaa ctggaagagg cgctgaacgt gctgctggaa gatgttcgcg
    30421 ccaggccaca acaaatcctt gaaagcgacg ccgaagatat ccatagctgg gtggaaggca
    30481 aactgatcga caaagtgggc cagttaggca aaaagctgca taccgggcgt agtcgtaacg
    30541 atcaggtagc gactgacctg aaactgtggt gcaaagatac cgttagcgag ttactgacgg
    30601 ctaaccgaca gctgcaatcg gcgctggtgg aaaccgcaca aaacaatcag gacgcggtca
    30661 tgccaggtta cactcacctg caacgcgccc agccggtgac gttcgcgcac tggtgcctgg
    30721 cctatgttga gatgctggcg cgtgatgaaa gccgtttgca ggatgcgctt aagcgtctgg
    30781 atgtcagccc attaggctgt ggcgcgctgg cgggaacggc ctatgaaatc gaccgtgaac
    30841 agttagcagg ctggctgggc tttgcttcgg cgacccgtaa cagtctggac agcgtttctg
    30901 accgtgacca tgtgttggaa ctgctgtcgg ctgccgctat cggcatggtg catctgtcgc
    30961 gttttgctga agatctgatt ttctttaaca ccggcgaagc ggggtttgtg gagctttctg
    31021 accgcgtgac ttccggttca tcattaatgc cgcagaagaa aaacccggat gcgctggagc
    31081 tgattcgcgg taaatgcggt cgggtgcagg gggcgttaac cggcatgatg atgacgctga
    31141 aaggtttgcc gctggcttac aacaaagata tgcaggaaga caaagaaggt ctgttcgacg
    31201 cgctcgatac ctggctggac tgcctgcata tggcggcgct ggtgctggac ggcattcagg
    31261 tgaaacgtcc acgttgccaa gaagcggctc agcagggtta cgccaacgcc accgaactgg
    31321 cggattatct ggtggcgaaa ggcgtaccgt tccgcgaggc gcaccatatt gttggtgaag
    31381 cggtggtgga agccattcgt cagggcaaac cgctggaaga tctgcctctc agtgagttgc
    31441 agaaattcag ccacgtgatt ggcgaagatg tctatccgat tctgtcgcta caatcgtgcc
    31501 tcgacaagcg tgcggcaaaa ggcggcgtct caccgcagca ggtggcgcag gcgattgctt
    31561 ttgcgcaggc tcggttagag taatttgttt atattactcc cctgaaaatg gggagcttac
    31621 cacgcctaca aagtgcgaca cttcgtaggc ggataaggcg tttacgccac atcaggcaga
    31681 aatttgtcag cattctggtg accttaaata taacgcccaa ccagaaccat tatttgtcac
    31741 attccttttt atcattgtgt cttctttacc ttcacactcc ccatgaaaat catattttca
    31801 aatacatcaa ccaatacata ttttaattaa ttgaatataa ttattttcta ttgctgtgcc
    31861 catttttaat gattaatgga gtttgttatg tgattattta acgtgttgtt ttaacgataa
    31921 tggttaatta ttcaggtggt ttttgagggt attttttctc ctcggtggaa atttttgttt
    31981 ttaatgtgat cacggttgaa taatctcttt ttctctcttg tatggtagta ggcgtaaaat
    32041 taagtttgaa acgtctattt acaacacagg agattgcaat aatgaaaatt attgctgcag
    32101 atgtttttgt tacttgccca gggcgtaatt tcgttacgct taaaataacc actgaaagcg
    32161 gactttgtgg attaggagat gccacgttaa atggtcgcga attgtcggtc gcttcttatc
    32221 tgaaagatca tctttgtcca caactaattg gccgcgatgc cagccgtatc gaagatatct
    32281 ggcaattctt ttataaaggt gcttactggc gacgtggccc cgtcacgatg tcagccattt
    32341 ctgccattga tatggcgttg tgggatatca aagctaaagc ggcaaatatg ccattgtatc
    32401 aactgcttgg tggtgcttct cgcgaaggcg tgatggttta ttgccacacc accgggcgga
    32461 cgattgatga agtccttgaa gactatgcaa agcaccagca gatgggcttt aaggcgattc
    32521 gtgtgcagtg cggtgtaccg ggtatgcaaa ctacttatgg cctggcgaag ggcaaaggcc
    32581 tggcgtatga accggcaacc aaaggcctgt ggccagaaga gcagttgtgg tcttcagaaa
    32641 agtatctcga ttttacgccg aagttatttg aagcggtgcg taataagttc ggttttaacg
    32701 aacatctgtt gcatgacatg caccatcgcc tgacccccat cgaagcggcg cgctttggga
    32761 agagcattga ggattaccgc ctgttctgga tggaagatcc gacgcctgca gagaaccagg
    32821 aatgttttcg tctgattcgc cagcataccg tgacgccgat tgcggtcggt gaagtgttta
    32881 acagtatctg ggactgcaag cagttaatcg aagagcagct gattgactat attcgcacga
    32941 cgatcaccca tgcaggcggc attaccggga tgcggcggat tgccgatttt gcatcgcttt
    33001 atcaggtacg taccggttcg catggccctt cagatttatc gcctgtttgt catgcggcgg
    33061 cgctgcattt tgacctatgg gtaccaaatt ttggcgttca ggagtacatg ggctattccg
    33121 aacaaatgct tgaagttttc ccgcacagct ggcgcttcga ggaaggttat atgcacccag
    33181 gcgatgaacc ggggctgggt atttctttcg atgagaaact ggcggcgaaa tatccgtatg
    33241 acccggcgta tctccctgtc gcacgactgg aagatggcac attgtggaac tggtaataac
    33301 ggttcgctta attattttct gaatagtcat caaatcgatt aattccgctc aaggagctaa
    33361 aaatggacgt tgatgtgtca accagtgttg cgggtaataa accgcaacgg attcgtcgta
    33421 ttcagacagt gaccctggtt ttattattta tggcggggat cgttaatttt ctcgaccgtt
    33481 cgtcattgag cgtggcaggt gaagcgattc gtggcgagtt aggattatcg gcgacagagt
    33541 ttggtgtttt gctttctgca ttttctctgt cttatggttt ttcacaacta ccttccggta
    33601 ttttgctaga ccgctttggc ccacgaattg ttttaggcgc aggcttaata ttctggtcat
    33661 taatgcaggc attaacagga atggttaatt cttttagcca ctttatttta atgcgtatcg
    33721 gtctgggtat tggcgaagcg ccatttatgc ctgcgggagt aaagtcgatc accgactggt
    33781 atgcacaaaa agaacgcggc acggcgctgg ggatttttaa ctcatctacc gttatcggtc
    33841 aggctatcgc gcctcctgct cttgtattga tgcagctggc atggggctgg cggacgatgt
    33901 tcgttatcat cggcgtggca gggattctgg ttgggatctg ttggtacgcg tggtatcgca
    33961 accgggcgca gtttgtcctg actgacgaag aacggacgta tctctccgcg ccggttaaac
    34021 cgcgtccaca gctgcaattt agcgagtggc tggcgctgtt taagcatcgg acaacctggg
    34081 ggatgatttt aggtttctct ggcgtcaatt ataccggttg gctctacatc gcgtggttac
    34141 ccggttattt gcaagccgag caaggtttca gtctggcgaa aaccggttgg gtggcggcga
    34201 ttcctttcct cgcggcggca gtcgggatgt gggttaacgg tattgttgtc gatcgactgg
    34261 cgaaaaaagg ctacgacctg gcgaagacgc gtaaaacggc tattgtctgc ggtttgatga
    34321 tgtcggcatt aggcacgttg ctggtcgtgc aatcttcctc gccagcccag gcggtagcgt
    34381 ttatctcaat ggcgctattc tgcgtgcatt tcgctggaac gtctgcatgg gggctggtgc
    34441 aggtgatggt gtcagaaaca aaagtggctt ccatcgccgg gattcaaaac tttggcagtt
    34501 ttgtttttgc ttcctttgct ccgatcgtaa ccggttgggt agtggatacc acacactcgt
    34561 ttaatctggc gttggttatc gcggcctgtg tgacgttcac cggggcgctg tgttacttct
    34621 ttattgtcaa agatcgcatt gagtaatcgt tactatcagg ctctcttgct gtgggcctgt
    34681 agaataaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
    34741 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
    34801 cctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattcttcc
    34861 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
    34921 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagactcct gccacgttag
    34981 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
    35041 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
    35101 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggtgagac
    35161 catgtccggg ccgctgcata ttggtttgat tcccacggtc gggccgtacc tgctaccgca
    35221 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
    35281 gacccaccag ttactggcgc aactggacag cggcaaactc gattgcgtga tcctcgcgct
    35341 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
    35401 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatggccg atctggcagg
    35461 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
    35521 ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg agacgctgcg
    35581 caacatggtg gcggcaggta gcgggatcac tttactgcct gcgctggctg tgccgccgga
    35641 gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
    35701 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
    35761 catccgcgca cgaatggatg gccatttcga taaagtgtta aaacaggcgg tttaaaccgt
    35821 ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg gtgttgacga
    35881 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
    35941 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
    36001 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc agatgtttaa
    36061 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
    36121 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgcg
    36181 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgccg
    36241 acgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
    36301 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
    36361 acgccagcga atcagtatta ccggtgcgac cgttggcata gagcaggcag tcagctttca
    36421 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
    36481 actcttcgtt gtggcgaatc actacgccac tgttccagaa gtgataggag agagaatctg
    36541 acatctcttg atcgagaaat gccagcagcc gatcgcgggt gttgatcaga tccactttta
    36601 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
    36661 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
    36721 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
    36781 cagcggttag tgtttcaacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
    36841 caaagcgagc gtttccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
    36901 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
    36961 ggagcagtcg ggaatggtcg ctgtaaagtg ggttttgatt gaattctata atgcggctga
    37021 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
    37081 tttgataacg ctcgataact gcgacgcgcg caccttgctt aaccaggccc attgcagcgc
    37141 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
    37201 tagggcttac ctgttcttat acataaaagc aacagaatgg taacatttta tcgcgggtaa
    37261 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
    37321 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
    37381 aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca
    37441 ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt
    37501 ggcaaggatt cagacatcgt gatgggcata agagcgcaac aaaaagaaaa aacccgccgt
    37561 tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt
    37621 ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc
    37681 gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc
    37741 atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac ctcggtctcc
    37801 acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc
    37861 tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg
    37921 gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa
    37981 gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc
    38041 gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg
    38101 gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag
    38161 gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg ttagttgctg
    38221 gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat
    38281 tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca
    38341 ctatgccggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt
    38401 acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac
    38461 tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg
    38521 ctgagtaatc ggtattatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc
    38581 agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt
    38641 tcgaccttgt gcgtctggct taatgtttcc agattcttgc ataacgtttc cgggttacag
    38701 gagatgtaca aaatacgcgg atacgcctgc accattttct cggtttcact gtccagaccg
    38761 ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac tctttaagtc gatcccttgc
    38821 aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga
    38881 ataatttgta cgttatcaat atggttagct gcgatgttgt attgcgcagc agcaaccgac
    38941 ggcttagcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag
    39001 ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc
    39061 gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaaact gttttctacc
    39121 tgacggtaga tcatctcttt ccctgcgacc ggcagacgtt catcgatgta atcttgatcc
    39181 agctcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc
    39241 agtgcatcgc gcagggcctc cgcttcctga cgccactcat catccagctt cttatggtat
    39301 agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg
    39361 tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgatg
    39421 agttcactgg cagcggggaa gctatccacg cggatgcggc ttttggtttg ttgatcgaaa
    39481 atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcacg catccggtaa
    39541 tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt
    39601 tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg
    39661 ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag
    39721 atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa
    39781 cctgtagcat ccacttgccg gtcctgtgag ttaataggga atccagtgcg aatctggagc
    39841 tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg
    39901 ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa
    39961 cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta
    40021 ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ctgtcacagc
    40081 cttttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg ctaaccgttt
    40141 tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga
    40201 ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac
    40261 ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca
    40321 tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg ggggtgagtg gttctgccga
    40381 ccttagccag ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg ggccacgctc
    40441 cgccgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga
    40501 acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc
    40561 tacgcagcaa caactggggg ataagacacg ggtaacgctg ttgggcgatt atgcccatac
    40621 tcatggttat gatgttgttg cctatggtaa taccggaacg caagcgcaga cagataacga
    40681 tggtttttta agtaaaacgc tttatggcgc gctggagcat aactttactg atgcctggag
    40741 cggctttgtg cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc
    40801 cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg
    40861 ctataacggc gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta
    40921 caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca
    40981 atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt
    41041 cgactggcag aaacagacta cgacgccggg tacaggttat gttgagaatg gatatgatca
    41101 acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg
    41161 cgcagcacgc agtgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc
    41221 cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc
    41281 accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag
    41341 caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg
    41401 atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa
    41461 cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc aattttgata ccggaccact
    41521 gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt
    41581 gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga
    41641 ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc
    41701 ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt
    41761 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac
    41821 agtctatggc taccaaactg caggacggga atacaccttg tctggcagct acaccttctg
    41881 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
    41941 agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac gtcgcttttc
    42001 cgtatggcga aaaaagcgaa gagtttattg ttgagcgagt ggtggcaatt gtcaccgcag
    42061 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacaccgcc agtaccgttt
    42121 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
    42181 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaactg
    42241 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
    42301 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctgcatggc gaagatgttt
    42361 ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
    42421 ccgttgtatt gggttgcacc catttccctc tactacaaga agaactctta caggtgctgc
    42481 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgcagaacg gcctggctgt
    42541 tagaacatga agctccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa
    42601 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
    42661 aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcggaaa attattttaa
    42721 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaat
    42781 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat tccgacagag aaagcaaaaa
    42841 taaatgcttg actctgtagc gggaaagcgt attatgcaca ccccgcgccg ctgagaaaaa
    42901 gcaaagcgg
//
LOCUS       DAZFVO020000023        40882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFVO020000023 DAZFVO020000000
VERSION     DAZFVO020000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 40882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.023"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(18..851)
                     /gene="fdhD"
                     /locus_tag="ACHKNB_RS05795"
                     /old_locus_tag="pgaptmp_001159"
     CDS             complement(18..851)
                     /gene="fdhD"
                     /locus_tag="ACHKNB_RS05795"
                     /old_locus_tag="pgaptmp_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HGU4194716.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1045..4095
                     /gene="fdnG"
                     /locus_tag="ACHKNB_RS05800"
                     /old_locus_tag="pgaptmp_001160"
     CDS             1045..4095
                     /gene="fdnG"
                     /locus_tag="ACHKNB_RS05800"
                     /old_locus_tag="pgaptmp_001160"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1630..1632,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4194717.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4108..5010
                     /gene="fdoH"
                     /locus_tag="ACHKNB_RS05805"
                     /old_locus_tag="pgaptmp_001161"
     CDS             4108..5010
                     /gene="fdoH"
                     /locus_tag="ACHKNB_RS05805"
                     /old_locus_tag="pgaptmp_001161"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HGU4194718.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5007..5642
                     /gene="fdoI"
                     /locus_tag="ACHKNB_RS05810"
                     /old_locus_tag="pgaptmp_001162"
     CDS             5007..5642
                     /gene="fdoI"
                     /locus_tag="ACHKNB_RS05810"
                     /old_locus_tag="pgaptmp_001162"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4194719.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5639..6568
                     /gene="fdhE"
                     /locus_tag="ACHKNB_RS05815"
                     /old_locus_tag="pgaptmp_001163"
     CDS             5639..6568
                     /gene="fdhE"
                     /locus_tag="ACHKNB_RS05815"
                     /old_locus_tag="pgaptmp_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HGU4194720.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6898..7140)
                     /gene="yiiF"
                     /locus_tag="ACHKNB_RS05820"
                     /old_locus_tag="pgaptmp_001164"
     CDS             complement(6898..7140)
                     /gene="yiiF"
                     /locus_tag="ACHKNB_RS05820"
                     /old_locus_tag="pgaptmp_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HGU4194721.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7360..7578)
                     /gene="yiiE"
                     /locus_tag="ACHKNB_RS05825"
                     /old_locus_tag="pgaptmp_001165"
     CDS             complement(7360..7578)
                     /gene="yiiE"
                     /locus_tag="ACHKNB_RS05825"
                     /old_locus_tag="pgaptmp_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGU4194722.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            complement(8164..8454)
                     /gene="nadS"
                     /locus_tag="ACHKNB_RS05830"
                     /old_locus_tag="pgaptmp_001166"
     CDS             complement(8164..8454)
                     /gene="nadS"
                     /locus_tag="ACHKNB_RS05830"
                     /old_locus_tag="pgaptmp_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HGU4194723.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8455..8766)
                     /locus_tag="ACHKNB_RS05835"
                     /old_locus_tag="pgaptmp_001167"
     CDS             complement(8455..8766)
                     /locus_tag="ACHKNB_RS05835"
                     /old_locus_tag="pgaptmp_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4194724.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            8995..9903
                     /locus_tag="ACHKNB_RS05840"
                     /old_locus_tag="pgaptmp_001168"
     CDS             8995..9903
                     /locus_tag="ACHKNB_RS05840"
                     /old_locus_tag="pgaptmp_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4194725.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(9967..10908)
                     /gene="fabY"
                     /locus_tag="ACHKNB_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /gene_synonym="yiiD"
     CDS             complement(9967..10908)
                     /gene="fabY"
                     /locus_tag="ACHKNB_RS05845"
                     /old_locus_tag="pgaptmp_001169"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HGU4194726.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10953..11390)
                     /gene="dtd"
                     /locus_tag="ACHKNB_RS05850"
                     /old_locus_tag="pgaptmp_001170"
     CDS             complement(10953..11390)
                     /gene="dtd"
                     /locus_tag="ACHKNB_RS05850"
                     /old_locus_tag="pgaptmp_001170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGU4194727.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11387..12259)
                     /gene="yihY"
                     /locus_tag="ACHKNB_RS05855"
                     /old_locus_tag="pgaptmp_001171"
     CDS             complement(11387..12259)
                     /gene="yihY"
                     /locus_tag="ACHKNB_RS05855"
                     /old_locus_tag="pgaptmp_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HGU4194728.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12253..12852)
                     /gene="yihX"
                     /locus_tag="ACHKNB_RS05860"
                     /old_locus_tag="pgaptmp_001172"
     CDS             complement(12253..12852)
                     /gene="yihX"
                     /locus_tag="ACHKNB_RS05860"
                     /old_locus_tag="pgaptmp_001172"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HGU4194729.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(12993..13880)
                     /locus_tag="ACHKNB_RS05865"
                     /old_locus_tag="pgaptmp_001173"
     CDS             complement(12993..13880)
                     /locus_tag="ACHKNB_RS05865"
                     /old_locus_tag="pgaptmp_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4194730.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(13870..15219)
                     /locus_tag="ACHKNB_RS05870"
                     /old_locus_tag="pgaptmp_001174"
     CDS             complement(13870..15219)
                     /locus_tag="ACHKNB_RS05870"
                     /old_locus_tag="pgaptmp_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194731.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(15318..16750)
                     /locus_tag="ACHKNB_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /pseudo
     CDS             complement(15318..16750)
                     /locus_tag="ACHKNB_RS05875"
                     /old_locus_tag="pgaptmp_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16820..17830)
                     /locus_tag="ACHKNB_RS05880"
                     /old_locus_tag="pgaptmp_001176"
     CDS             complement(16820..17830)
                     /locus_tag="ACHKNB_RS05880"
                     /old_locus_tag="pgaptmp_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HGU4194732.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(17863..18750)
                     /locus_tag="ACHKNB_RS05885"
                     /old_locus_tag="pgaptmp_001177"
     CDS             complement(17863..18750)
                     /locus_tag="ACHKNB_RS05885"
                     /old_locus_tag="pgaptmp_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HGU4194733.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18775..19653)
                     /gene="yihU"
                     /locus_tag="ACHKNB_RS05890"
                     /old_locus_tag="pgaptmp_001178"
     CDS             complement(18775..19653)
                     /gene="yihU"
                     /locus_tag="ACHKNB_RS05890"
                     /old_locus_tag="pgaptmp_001178"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HGU4194734.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19826..20722
                     /locus_tag="ACHKNB_RS05895"
                     /old_locus_tag="pgaptmp_001179"
     CDS             19826..20722
                     /locus_tag="ACHKNB_RS05895"
                     /old_locus_tag="pgaptmp_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4194735.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQENHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20762..21550
                     /locus_tag="ACHKNB_RS05900"
                     /old_locus_tag="pgaptmp_001180"
     CDS             20762..21550
                     /locus_tag="ACHKNB_RS05900"
                     /old_locus_tag="pgaptmp_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4194736.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21661..22926)
                     /gene="yihN"
                     /locus_tag="ACHKNB_RS05905"
                     /old_locus_tag="pgaptmp_001181"
     CDS             complement(21661..22926)
                     /gene="yihN"
                     /locus_tag="ACHKNB_RS05905"
                     /old_locus_tag="pgaptmp_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194737.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23028..24008)
                     /gene="yihM"
                     /locus_tag="ACHKNB_RS05910"
                     /old_locus_tag="pgaptmp_001182"
     CDS             complement(23028..24008)
                     /gene="yihM"
                     /locus_tag="ACHKNB_RS05910"
                     /old_locus_tag="pgaptmp_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HGU4194738.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(24016..24726)
                     /gene="yihL"
                     /locus_tag="ACHKNB_RS05915"
                     /old_locus_tag="pgaptmp_001183"
     CDS             complement(24016..24726)
                     /gene="yihL"
                     /locus_tag="ACHKNB_RS05915"
                     /old_locus_tag="pgaptmp_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4194739.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLATLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(24943..26766)
                     /gene="typA"
                     /locus_tag="ACHKNB_RS05920"
                     /old_locus_tag="pgaptmp_001184"
     CDS             complement(24943..26766)
                     /gene="typA"
                     /locus_tag="ACHKNB_RS05920"
                     /old_locus_tag="pgaptmp_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HGU4194740.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            27139..28548
                     /gene="glnA"
                     /locus_tag="ACHKNB_RS05925"
                     /old_locus_tag="pgaptmp_001185"
     CDS             27139..28548
                     /gene="glnA"
                     /locus_tag="ACHKNB_RS05925"
                     /old_locus_tag="pgaptmp_001185"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HGU4194741.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28721..29770
                     /gene="glnL"
                     /locus_tag="ACHKNB_RS05930"
                     /old_locus_tag="pgaptmp_001186"
     CDS             28721..29770
                     /gene="glnL"
                     /locus_tag="ACHKNB_RS05930"
                     /old_locus_tag="pgaptmp_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HGU4194742.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29782..31191
                     /gene="glnG"
                     /locus_tag="ACHKNB_RS05935"
                     /old_locus_tag="pgaptmp_001187"
     CDS             29782..31191
                     /gene="glnG"
                     /locus_tag="ACHKNB_RS05935"
                     /old_locus_tag="pgaptmp_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HGU4194743.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            31303..31413
                     /gene="yshB"
                     /locus_tag="ACHKNB_RS05940"
                     /old_locus_tag="pgaptmp_001188"
     CDS             31303..31413
                     /gene="yshB"
                     /locus_tag="ACHKNB_RS05940"
                     /old_locus_tag="pgaptmp_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HGU4194744.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(31603..32976)
                     /gene="hemN"
                     /locus_tag="ACHKNB_RS05945"
                     /old_locus_tag="pgaptmp_001189"
     CDS             complement(31603..32976)
                     /gene="hemN"
                     /locus_tag="ACHKNB_RS05945"
                     /old_locus_tag="pgaptmp_001189"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HGU4194745.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33165..33674)
                     /gene="yihI"
                     /locus_tag="ACHKNB_RS05950"
                     /old_locus_tag="pgaptmp_001190"
     CDS             complement(33165..33674)
                     /gene="yihI"
                     /locus_tag="ACHKNB_RS05950"
                     /old_locus_tag="pgaptmp_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HGU4194746.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(33793..33959)
                     /locus_tag="ACHKNB_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /pseudo
     CDS             complement(33793..33959)
                     /locus_tag="ACHKNB_RS05955"
                     /old_locus_tag="pgaptmp_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34256..34888
                     /gene="yihA"
                     /locus_tag="ACHKNB_RS05960"
                     /old_locus_tag="pgaptmp_001192"
     CDS             34256..34888
                     /gene="yihA"
                     /locus_tag="ACHKNB_RS05960"
                     /old_locus_tag="pgaptmp_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGU4194747.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35270..38056)
                     /gene="polA"
                     /locus_tag="ACHKNB_RS05965"
                     /old_locus_tag="pgaptmp_001193"
     CDS             complement(35270..38056)
                     /gene="polA"
                     /locus_tag="ACHKNB_RS05965"
                     /old_locus_tag="pgaptmp_001193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGU4194748.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            38420..39352
                     /gene="yihG"
                     /locus_tag="ACHKNB_RS05970"
                     /old_locus_tag="pgaptmp_001194"
     CDS             38420..39352
                     /gene="yihG"
                     /locus_tag="ACHKNB_RS05970"
                     /old_locus_tag="pgaptmp_001194"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4194749.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(39393..40823)
                     /gene="yihF"
                     /locus_tag="ACHKNB_RS05975"
                     /old_locus_tag="pgaptmp_001195"
     CDS             complement(39393..40823)
                     /gene="yihF"
                     /locus_tag="ACHKNB_RS05975"
                     /old_locus_tag="pgaptmp_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4194750.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        10242 a        10560 c         9914 g        10166 t
ORIGIN      
        1 cgcaggactt attttaatta attgctcaaa cgctgcggat gggtataaac cgttgcccga
       61 cccggtttac aaaaacctac cagtgtcaga ttacagcgct cagccacttc tacagcaagc
      121 gtggtcgcgg cagacaccgc aaacaaaatc tctacgccgc acatcgccga cttttgcacc
      181 atttcataac tggcacgact ggaaaccagt accgcacctt gctgccagct ttccccttct
      241 tgtgaccggc ggcctaacag tttgtccagc gccacatggc gacccacgtc ttcatgcccg
      301 ccgaccagtt cgccagatgg caacatccag gcagcggcgt gagtacaacc agtcagttgc
      361 cccactggct gaaaatcgtt gagatgacgt aatgcatcat ccagtttgtt gagatcaaac
      421 gtctgggtga acggtagcgg ctgcaccggt tttccgatgt cattaagttg ctccacgccg
      481 catacgccac agcccgtacg tcccgccagc gcccggcggc gctccttcaa ccccataaag
      541 cggcggctgg aaagctcaat ttgtacttca agaccattac aggaaggaac gacatccatg
      601 ccgaagatat cgcgcggact ttctattatc ccctcggaaa gcgaaaaacc gagcgcaaag
      661 tactcaaggt ctttgggcga cgccatcatc accacatgcg aaatgccgtt gtagaccagc
      721 gcaacgggaa cttcttccgc gacctcatct aaccgtgggt gttgtaaatc atcacgccgc
      781 cataactcaa tttgacgaac acctgtgata tttgtcacat tttcgatttc tttttgctgt
      841 gtttttttca ctttctctta accagataag aacacacaca ccaacattat ggtattctgt
      901 tacaaaccct tcctggatgg agggaaattg agccaattct ggacctttgc ggccccttcc
      961 gcaaagaaaa ataactccca ctccctgcac atgcagcaag cgaatgtaaa tgggacgtga
     1021 caatgtcgaa acaaggagca atccatgcag gtcagcagaa ggcagttctt taagatctgc
     1081 gctggcggta tggcaggcac cacggcagcg gcactgggtt ttgcacccag cgtagcactc
     1141 gcggaaaccc ggcagtataa actgctgcgc acccgcgaaa cccgtaatac ctgcacctat
     1201 tgttccgtag gctgtgggct gttgatgtac agcctcggtg acggagcaaa aaacgccaaa
     1261 gcatctatct tccatatcga aggcgatccg gatcacccgg tcaaccgcgg tgcactttgt
     1321 ccgaaaggcg ctggcctggt ggatttcatc cactccgaaa gccgtctgaa gtttccggaa
     1381 taccgtgcgc caggttctga taaatggcag caaatcagtt gggaagaggc gtttgatcgc
     1441 atcgccaaac tgatgaaaga agaccgcgat gctaactaca ttgcgcaaaa cgccgaaggc
     1501 gtgactgtta accgctggct ctccaccggg atgctgtgtg cttccgcgtc gagtaacgaa
     1561 accggctatt taacgcaaaa attctcccgc gcgctgggta tgctcgcggt cgacaaccag
     1621 gcgcgtgtct gacacggacc aacggtagca agtcttgctc caacatttgg tcgcggtgcg
     1681 atgaccaacc actgggtcga catcaagaac gccaacctcg tcgtggtgat gggcggtaac
     1741 gccgctgaag ctcacccggt cgggttccgc tgggcgatgg aagccaaaat tcacaacggc
     1801 gcgaagctga ttgtgatcga tcctcgcttt acgcgtacgg cggcggtagc tgactactat
     1861 gcccctattc gttccggtac tgacattgct ttcctgtcag gcgtattgct gtacctgctg
     1921 aacaatgaaa aattcaaccg cgaatacacc gaagcctata ccaacgccag cctgatagtc
     1981 cgtgaggatt acggctttga agatggcctg ttcaccggct acgacgcgga aaaacgcaag
     2041 tacgataaat cctcctggac ttatgaactg gacgaaaacg gcttcgccaa acgcgatacc
     2101 acgctgcaac atccgcgctg cgtgtggaac ttgctgaaac agcacgtttc ccgctacacg
     2161 ccagatgtgg ttgaaaacat ctgtggtacg ccaaaagacg cgttcctgaa agtctgcgaa
     2221 tacatcgcag aaaccagtgc tcacgataaa actgcctcgt tcctgtatgc cctcggctgg
     2281 acgcaacact ccgttggtgc gcaaaacatt cgtacgatgg cgatgatcca gctgctgctc
     2341 ggcaatatgg ggatggccgg cggcggcgtt aacgccctgc gcggtcactc caatattcag
     2401 gggctgacgg acctggggct actgtcgcag agtctgccag gttacatgac gctgccaagc
     2461 gagaagcaga ccgatctgca aacctacctt accgccaaca cgccaaaacc gctgctggaa
     2521 ggccaggtaa actactgggg caactacccg aaattcttcg tctctatgat gaaggccttc
     2581 tttggtgata aagcgacggc ggaaaatagc tggggctttg actggttgcc gaagtgggat
     2641 aaaggctacg acgtcctgca atacttcgag atgatgaaag aaggcaaggt caatggctat
     2701 atctgccagg gctttaaccc tgttgcctca ttcccgaaca aaaacaaagt gatcggttgt
     2761 ctgtcgaaac tgaagttcct cgtcaccatc gacccgctga acactgaaac ctctaacttc
     2821 tggcagaacc acggtgagct gaacgaagtt gactcgtcga agatccagac cgaagtgttc
     2881 cgtctgccat cgacctgctt cgcagaagag aacggttcaa tcgttaactc aggtcgctgg
     2941 ttgcagtggc actggaaagg tgcggacgcc ccggggattg cgctgactga cggtgagatc
     3001 ctctccggta tcttcctgcg cttgcgcaag atgtatgccg aacagggtgg cgcgaacccg
     3061 gatcaggtgc tgaacatgac ctggaactac gccattccgc atgagccgaa gtcggaagaa
     3121 gtagcgatgg agagcaacgg taaggcgctg gccgatatta ccgatccggc aaccggtgcg
     3181 gttatcgtca agaaaggcca acaacttagc tcgttcgccc aattgcgcga tgacggtaca
     3241 acctcctgtg gctgctggat tttcgccggt agctggacgc cggaaggcaa ccagatggcg
     3301 cgtcgtgata acgccgatcc gtctggcctc ggtaacacgc tgggctgggc atgggcatgg
     3361 ccgcttaacc gccgcattct gtataaccgc gcctccgcag atccgcaggg taacccgtgg
     3421 gatccgaagc gtcagttgct gaaatgggac ggcactaagt ggaccggctg ggatattccg
     3481 gactacagcg cagcgcctcc gggtagcggc gtcgggccgt ttatcatgca gcaggaaggc
     3541 atggggcgtc tgtttgccct cgataagatg gcggaaggtc cgttcccgga acactacgag
     3601 ccgtttgaaa cgccgctggg aactaacccg ctgcatccaa acgttatctc gaacccggct
     3661 gcacgtatct ttaaagacga cgccgaagca ttgggtaaag ccgataagtt cccgtatgtc
     3721 ggaaccacct atcgtctgac cgagcatttc cactactgga ccaaacacgc gctgttgaac
     3781 gcgattttgc aaccagagca gtttgtggaa atcggggagt cgctggcgaa taaacttggc
     3841 attgcccagg gcgataccgt gaaagtctcc tccaaccgtg gctatatcaa ggccaaagcg
     3901 gtggtgacca aacgtattcg tacactgaaa gcgaacggca aagatatcga taccatcggt
     3961 attccgattc actggggcta tgaaggcgta gctaaaaaag gctttattgc caatacgtta
     4021 acgccattcg ttggtgatgc gaacacgcag acgccggagt ttaagtcctt ccttgtgaat
     4081 gtggaaaagg tgtaacggag acgacatatg gcttatcaat ctcaagatat cattcgtcgt
     4141 tccgcgacta acggtctgac ccccgcgcct caggcgcggg acttccagga agaagtggcg
     4201 aaactcatcg acgtcaccac ctgtatcggc tgtaaagcct gtcaggtggc gtgttcagag
     4261 tggaacgaca ttcgcgatac cgtcggcaat aacattgggg tgtacgacaa ccccaatgat
     4321 ttaagcgcca aatcgtggac ggtaatgcgc ttctcggaag tggagcagaa cgacaaactg
     4381 gaatggctga tccgcaaaga cggctgtatg cactgttccg atccaggctg cctgaaagcg
     4441 tgcccggcgg aaggtgcaat tattcagtat gccaacggta tcgtcgactt ccagtccgag
     4501 cagtgcattg gctgcggtta ttgcattgcg ggctgtccgt tcgacattcc gcgcctcaac
     4561 ccggaagaca accgcgtcta caaatgtacg ctgtgcgttg accgcgtggt ggttgggcaa
     4621 gaaccagcct gcgtgaagac ctgcccaacg ggcgcgattc acttcggtac gaaagagtcg
     4681 atgaaaacgc tggcgagcga gcgcgttgct gagctgaaaa cccgcggtta cgacaatgcg
     4741 ggtctgtacg atccggcagg cgtcggtggt acacacgtca tgtacgtgct gcaccatgct
     4801 gacaagccaa atctgtatca tggcttgccg gagaacccgg aaatcagcga aaccgtgaaa
     4861 ttctggaaag gcatctggaa accgctcgcg gctgttggct ttgcggctac ctttgcggcc
     4921 agtatcttcc actacgtggg tgtcggtccg aaccgtgcgg atgaggaaga gaataatctg
     4981 cacgaagaga aagacgagga gcgcaaatga aacgacgtga caccatcgtg cgctacaccg
     5041 cgccggaacg tatcaaccac tggatcaccg ccttctgctt catcctggcg gcggtgagcg
     5101 ggctgggctt tttgttcccg tccttcaact ggttgatgca aatcatgggc acaccgcagc
     5161 tggcgcgaat tctgcacccg tttgtcggcg tggttatgtt tgcctcgttc atcatcatgt
     5221 ttttccgtta ctggcatcac aacctaatca atcgggatga tatcttttgg gcgaagaata
     5281 ttcgtaagat cgtcgtcaac gaggaagtag gtgacaccgg gcgttataac ttcggtcaga
     5341 aatgcgtttt ctgggcggcg attattttcc tggttctgct gctggtgagc ggcgtgatta
     5401 tctggcgtcc ttattttgcg cctgctttct caatcccggt gatccgattc gcgttaatgc
     5461 tgcattcatt tgccgcagtg gcgttaattg tggttatcat ggtgcatatc tacgccgccc
     5521 tttgggtgaa aggcacgatt accgccatgg tggaaggatg ggttaccagc gcctgggcga
     5581 agaaacatca cccgcgctgg taccgtgagg tccgcaagac aacggaaaag aaagctgaat
     5641 gagtattcgc ataatcccgc aagatgagct gggttcgagc gagaaacgta cggcggatat
     5701 gattccgccg ttattgttcc ctcggctcaa gaatttatac aaccgccgcg ccgagcgtct
     5761 gcgcgagctg gcagaaaata atccgctggg tgattacctg cgctttgctg cgcttatcgc
     5821 ccacgcccag gaagtggtgc tgtacgacca tccgctggag atggatctga ccgcgcgcat
     5881 taaagaagcc agcgcacagg gcaagccccc gctggatatt cacgttctgc cgcgtgataa
     5941 gcactggcaa aagctgctga tggcgctgat tgctgagctg aaacctgaaa tgagcggccc
     6001 ggcgctggca gtgattgaga atctggagaa ggcatcaact caggagctgg aagatatggc
     6061 cagcgcactg tttgcctctg atttctcatc cgtcagcagc gataaagcgc cgtttatctg
     6121 ggctgcactg tcgctctact gggcgcagat ggccaatctg atccccggca aagcccgcgc
     6181 tgaatacggc gagcaacgtc aatattgccc ggtttgtggt tctatgccgg tgtccagcat
     6241 ggtgcaaatt ggcaccactc agggtctgcg ttacctgcac tgcaacctgt gtgaaaccga
     6301 atggcacgta gtgcgcgtaa aatgcagcaa ctgcgagcag agcggcaaac tgcattactg
     6361 gtcgctggat gacgaacagg ccgcgattaa agccgaaagc tgcgatgact gtggcactta
     6421 cctgaagatt ctttatcagg aaaaagaacc gaaagttgaa gccgtggcag atgacctcgc
     6481 ttctctggta ctggacgcgc gaatggagca agaaggctat gcccgcagtt ccatcaaccc
     6541 gttcctgttt ccgggtgaag gggagtaatc tctgacgttt tccgggtggc gctggtcgcc
     6601 cggattacag ctcatagggt gtgacgacac catctctcgt attccgcgtt acccattcaa
     6661 gatcatgccg caattgtctg tttgcctcgt tcttccctcc aacacatcga taaggttatg
     6721 ggtatgtcac tgatgacaag cgtatgattg cctgatgcgc tgcgcttatc agccctacca
     6781 ataaacttca atatattgaa tttgcatgac tttgtaggcc ggataaggcg ttcacgccgc
     6841 atccggcatg aacaacgcgc acgttgtcag caatctgatt gacatcattt tttcccatta
     6901 ccattcctcg cgcagcttac gctgatattc gacaccatct tcctcacacc cttgccagat
     6961 gccaggaaca ctggttcttg ctgtttgcga ttgatttatc aggtattgat ctaccgcttc
     7021 ccttaatagg tccgcacgag gaagattacg ctgcacctcg agatcatcaa gttgcttaat
     7081 cacctcattc gataaatcga gtaaaattct gcccatatct atacctacca gagagttcat
     7141 aaatatctcg ccaatatcat ttttgaatct atggagagaa aagtaccctt gtcgaatctt
     7201 taaagaaagc tcatttacga atcatttttt atttttgcga ggcgttttcc agatatgatg
     7261 taacagttgc agttattata agttcaagta aaacaacctt gtattaatac cagaataagg
     7321 gcatctggtt ttttatattc aagaataaaa aaatttttgt cattccttat gatccttaca
     7381 ctgatcataa acacctactg tctcacccct ttgcctgcct ctctttgctt tacgcaccgg
     7441 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
     7501 cttacgccag ccccgaagct tgttcagccg tttaattgcc tcttctgata aatgaagaaa
     7561 aaccgtattc atcgccatta ccaccacctt acatatatta ctcattaatg tatgtgcgaa
     7621 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
     7681 ggtgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
     7741 tagttatcgc ccaggatgag ttatggatgc tatgaatata ttatattcag agatatttat
     7801 ttgcataatc tgaatggtgt agccgaaaaa cgtcagcatc ctaacagcac caggatttaa
     7861 ggtgaaatta atcatccatc acagaccggt cagtactgcc accaaccggt ctttacttgt
     7921 tcatcgttat aattttgttg gtaacagttc gggaaacaag gaattccttc cggaaccccc
     7981 cggtatcatc cgatagcatt gacgtcaatc atcagcgcga cgatgagttt ttttaatcgc
     8041 atcatagggt ggcgacgttc agcacgatta cgggtcatag caaaactcct gggcaggcga
     8101 ccactatgcc gctttcacag gagttctgaa tgctagtgct ttctgtatgg cataaccatg
     8161 cctctaacgg ttaagtgctt gcagcacatg ttgcggatca ttggcaatcg ctcgcagcaa
     8221 agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc cagttacgca acgtgtctac
     8281 gcttacagaa ataagatcgg caaatttaga ttgtgacaac cctgaagcgc ggcgtatttc
     8341 tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc tgtcgcgttc cttcaataat
     8401 ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat ccatttacca
     8461 cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat ctttaatacc
     8521 cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa agtaaataac
     8581 cctgacacca gcacgtttcc cctttcctcc tgaaacccat cgaacctttc ttaacccacc
     8641 agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact gaaaacgact
     8701 gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg tttcgatgaa
     8761 gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc attgacgtac
     8821 ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg ccgggtttgt
     8881 ctttagattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc tgtatccatc
     8941 cagcaaaaac gggtgtataa gactgtacat tcatacagca aaataagttc tactatggca
     9001 ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg ctgcgggtcg cccctcatcg
     9061 cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt ccttgcgggt
     9121 gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgac cttacgaata
     9181 gtgtcgccgc tgaatgcccc gacattactc ccgacgataa tttattacta cggtggctgt
     9241 tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc ctattacggg
     9301 caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg aacatacttt ccccgccgcc
     9361 catgatgatg cgcaacgagg tgcagaaata gtctggcaac acgccgaacg actcggcgtt
     9421 gataaacaac gtataactct tgccggtgac agtgcgggcg gacatctggc gctggtaact
     9481 gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt aatttatcct
     9541 atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga ttacatcatc
     9601 acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga acgcaggcat
     9661 cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt acatatcatt
     9721 accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg catgacaggg
     9781 caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt ttttcagtta
     9841 ggtggtatca gcgaggccgc gcgatcggca ctacgggatg ttgcctggag agcgggaagg
     9901 taacttcgga cctgggccct gaaaaggttt tcagattgaa aaccttttca gtcaagtaaa
     9961 acgcccctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
    10021 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcaacttg
    10081 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
    10141 aaggtcggct accgcatgag gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc
    10201 cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga taagccccca
    10261 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
    10321 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatacgcac
    10381 gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca
    10441 gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca aaaaatggcg
    10501 aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca gcttggcgaa
    10561 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
    10621 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtccgggtg
    10681 aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacagtc ggcctaccgc
    10741 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
    10801 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
    10861 gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag cgatactctc
    10921 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
    10981 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
    11041 tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
    11101 gcgcctttgg agaaacttgg gcgcatccct cgttcggtat ctgcggcgag ggtaaactgg
    11161 gaaaccacca gtacactgcc gcccgcctgt tgcacgttga gattcatctt gccttcggca
    11221 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
    11281 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
    11341 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
    11401 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
    11461 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
    11521 ccgccagcac gccgtaaatg agctgatatg acgggaacat ggtgatataa agcgcgaaac
    11581 ctttctttcc tgcttcgaac aggagtgcag cgacaaacgc gccgacaatc gcgtcgcggt
    11641 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
    11701 gcagcggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc gcccagcgca
    11761 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
    11821 aaatcatcca gtacacggca aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
    11881 tggtattcaa cgcgctatcg atggagtaca tcaacaataa tgcggtgacg atcagcccgc
    11941 acgcgccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata taccgctgaa
    12001 taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgcaac tgaatgctga
    12061 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
    12121 atgagagcaa agacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
    12181 tacgttgcca gagtagtttt agccaggccc atagtggacg agtacggtgc ctggctttgt
    12241 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
    12301 caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg cgttatcgtc
    12361 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
    12421 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
    12481 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
    12541 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
    12601 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
    12661 ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct catgctgatg
    12721 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
    12781 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
    12841 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
    12901 gggtttggct atgtggcaat aagggtaatc ttgtgatgct gattgcgaaa aagcagagta
    12961 atttcactgg ctttttcgca atagatgaat ggttaagatt caggtgtaaa ttgccaacga
    13021 gtttcgatga ccagcggcct atctggtgtc accaattccg gcgttagatt tattgcgtta
    13081 ggtgcctgag tcagcgggtt aatacaggtg gcatcgggct gtttatcaaa taccaccaga
    13141 ctgtttgcag aagagatcat ggtcatcgcg agtttaccag gccacagtaa cttaaccttc
    13201 accccgtcat aaaaaccaaa acagtcatcc cagggaccgg cttgaggaga gatacgattt
    13261 ccagttggca gttcatcact acccgcttct tcttgccagt ccgcatcaaa aagcacctgc
    13321 aacgattctg tattttgcgg agtcagcttt ttcgcaaacc acggatgcca gcctgcggag
    13381 gccgggaatg tatcggcata tgaatggact tccagttgca aaaccagcgc atcgttctcc
    13441 agcaaaaaag tctgaatcac ttccccttgc cagggccacg gtgaagccag gggcatacgt
    13501 aaagtcaggg aatctgctgt tttgtcgatg atttcccacg ttgagtaaca agccatacca
    13561 tgcaacgcat gtggtggttt attggcgggt aaagaataac attgcccacc agcattcagc
    13621 gtcgcattcc ccagtcgccc tgcccacggc accattggga aacagccata ctgaaaagct
    13681 ctgcgctgcg gctgccattg gcgtaatact tcagaaccaa atgcctttaa cgaggttatc
    13741 ctggcaccat catgcggata aatctgcagg tgaatattgc ctttttccag cgtcactaac
    13801 tctcctacag gcccaggcca tggcgcgtca aacgcctgtt gtagcgccag gtaatgctcg
    13861 ttttttcgtt cagttttcat gagattgttc cctgatgtag cggtaagaag cggtttcttg
    13921 ttcatcggtc ggctcaatca gccagacgca aacaaaggaa agaactgaga cagcaaataa
    13981 aaacagagcg acataccacg gttttcctcc ccctagcgcc agcaaacttg aggcgataag
    14041 ggggcttagt ccgccaagaa tcgcggaaaa ttgataagca aaagaaaggc cggtatatcg
    14101 tactttggta ccgaacatgc gggtgaaaag tgttggctgc acggcaaaca tcatcgtcgg
    14161 acctaagttg tagccgagga tcatactgca ccagatgatg agcgtacttt tgctatccag
    14221 taataagaag aaaggaaacg cgaacaggac acaaaatcct gctcccgaca aatacagact
    14281 tttttgacct atacgatctg ataaccatcc aaccaatgga caactgaaaa taccgatcgc
    14341 cgaggcaatc aacatacccg tcaggggaat atctcgcgac aatgctaatt ggctggaaat
    14401 atagacaata ccaaaggcgt taataatatt agaggagaca ctttccgcca gcctcgcacc
    14461 cagtgccaat aaaatatttc gtggatgttt tttgaataat tcaatcaacg gaggaacgga
    14521 cttttcttta ttattcgtcg ttttttgctt ttgaaaatcc agcgtttctt cggtatgcag
    14581 gcggataaac agcccaacga tcaacatcac cgcagaaagc caaaacggaa tgcgccatcc
    14641 ccaggagaga aactgctccg aagtaagaaa atggttacag agagcaaaaa taccggttgc
    14701 cagcataata cccacagacg cagccgtttg cgggagtgaa cctaaaaatc cgcgtcggga
    14761 ttcgggcgct gattctatgg tcatcaatgc cgctccgcca tactcgccac caagaccaaa
    14821 gccctgaatt aaacgcagcg ccattaatat gagcggtgcc cagatcccaa tttcctgata
    14881 cgtcggaata aaaccaatca aaaatgtgga gcaaccgaca atagccagtg tccagataag
    14941 tgttattttt cgcccaattt tatcgccaaa atggccaaag acgataccac ctaaaggacg
    15001 ggtcaaaaaa ccaacgccaa aggtagcaaa ggcggctata agacctataa aaggatcgct
    15061 gtctggataa aacagcggtg caaataccag tcctgctgcc gtgccaaaaa taaagaagtc
    15121 ataccattcc attaacgcgc ctgcgaaact gcccataacg acacgtctca tggctttacc
    15181 ggaaagccct tcacgcggtg cgggtaactt ttcagacata attttcctct cctggtaggc
    15241 gagaaatctg ggatacgaac cactaaacgt gggcagtttg aattaatgcg catcggttta
    15301 agccgatgcg cattaattta tttcttcaat tcagccagaa cctctgcatc ctcagcatca
    15361 acatttgcac ccagtggttc ccaattaatc aatacgcagg tcaggaaccc caagaatggc
    15421 acgattgcca accagtaaaa cgcttcagtt ttcaacgccc ccacagcatc ggccagaaaa
    15481 tgagtcctgc aattgcccct gtgcgcataa tagcgcgggc aaatccgacc ccagtagggc
    15541 gaatagcggg aggataagag agtgtggcaa tggtcatgcc cagcccacca ggacccgctg
    15601 aatggaagaa aataattgat cccaataacc ctaaagacag ccatggatta gtcgttgcca
    15661 gtgcgcctaa cgacagcaat gctaaggtca caacagcaaa tccatacatc gactgtcggc
    15721 gagtacccag tcgttggaga ataaacgagc cgatcatccc gcctgtcacc ccacaaaggg
    15781 cattcacgac ggcagaaccc gtcaacgcac cagtaagccc gccacttatt attcccgcca
    15841 acgtaagagg aagataaaca cctacggcgt tatattgcca cgcctgcatg gttgccacga
    15901 cacagccaag aatggtgcgt ttgcggtaac gatcattaaa taagatgcca tatccaccac
    15961 tgtatttatt ctctgctttt tccgagaatt tcgcttctgt tgtactcgat gccgcaacgc
    16021 gagcctgaac accataacgt tttcccagaa tgtcgcacgc ttcctggtaa cgcccgacgc
    16081 gtgcggccca cattgcggat tcaccaataa agaagtaacg caaaatcatg aagatgactg
    16141 caaaaatagc tccgatgaat attccgtaac gccacaattg ttcttcagcg atacccgaca
    16201 gaagcagcgg caagagcacc aaataaacaa cagttgtcga aacataccag gccatttgcc
    16261 agaggttgac cgacgttccg gttttcttcg acgttgaacc acgaagctct gccacggcat
    16321 tggttgccaa agggaagtcg atgcctaaac caaagcccat gatgacgcgg cacaccagca
    16381 cccaccagat actgggcgca atagctaccg cagcagcccc gatggtgcac agccccatgc
    16441 caataataaa tgctcgcttt tgcccaaagc ggtttattac tcggttcgct aataaaccgc
    16501 caattaacgc ggcgattaat actgacgcat taacggttgc ggcaaggcct ggagaaatgc
    16561 caaattgagc ggcaatgtat ttattaccaa accccaccat cgccgcctgg taggcatcaa
    16621 tcaatatgcc gcccaaagca ataaaaataa cgccgatact ggtgttgatt gcagagttct
    16681 tattaacaat atcaattacg tcctgtggcc gactaattga ataagttgca ggactggtga
    16741 tttgactcat cgcagtgtcc tttttaattg ttgtagggtg gcgctgacgt gacgcagcgc
    16801 cagacagaga tttagaaaat cattccggcg caaattgcag catgaccttt atggagtcat
    16861 tttgctgcgc gcgttcgaat gccgccgctg catcctcaat ggcaaataaa tcggtaacca
    16921 gttcactggt atcaaaccgc ccatccgcca gccattcgat aacagcatcg aaatcttcag
    16981 ggatgtacat gccagagttg agcaagtcac gctcgaaacg ttgcatcatc ggtaatggta
    17041 tttcacgcgg cccacttggt acgcccatgc acacgacggt gcctccggca tatacacggg
    17101 tacatgcatc gttaagcgtg gcctgcgctg caacgacatc aatcgccccc gtaaatcgca
    17161 catctgcggc cagctcacct ggtgcccaaa cttctgctgc gcccagcttt aacgccagcg
    17221 cgcgtttggc agcgtcaggt tcaatcacgg taatcttacc tgcccccata atgcgcagtg
    17281 cctgcacgat ggataagcct atcgttccgg caccaatcac cagtacgctt tccagagaag
    17341 ctttcggcat tcggttaacg cagtgacggg cacaagccgc cggttcggca aaggccaaca
    17401 cttttagcgg taaagaggct ggtgcgacat ggcaattacg cgcaggaaca atgtgttgtg
    17461 agcgagcaaa gcccggtgcg cgaaaaccag caacctgcgg tggttcacaa agattaaaac
    17521 gtcctgcttt gcaggctcgg cattccatgc aagccatgat gggatcgacc acaacatgat
    17581 cgcccggctg tacattttta acgtcgctgc caacttccgt aacgcgcgct gcaatttcat
    17641 gaccggggac gacaggtggt ttagcaaacg gatgaccgcc tttcagaaca tgcagatccg
    17701 aaccgcatat cccatagaac acaggcgcaa tccgcacttc gtgcggttta agttcctggt
    17761 gtggtacttc aacatattga gtggtgactt tcccaatatc tgaaaataaa acctgggtaa
    17821 ttttatccat tgttattctc cagaatcgat atccataggg gattagcgtt gcgcgatacc
    17881 ttcatcgaca atttctgcca accgttgtaa acgaggaaca gagacatcgc gcagcatagt
    17941 ttgtggatct tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca ggaagcctga
    18001 cgcaccacct ttcatggcaa tacttactgc acggccaaaa atatcggcat caacgccaga
    18061 agaaagaatt acccacggca tcttcatctg atcgttaagt tgctgacagg cggcaagtaa
    18121 ggttttttca tcgcctttgc cccctaatgg catctcggct ttgtaaagat cagcctctgt
    18181 tccaccaagc tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg
    18241 acgaggtgga cgcaccacag gttcaataat gctaaccagc ccggcactac ggcagcgacg
    18301 aacaaattta tcaaccattg ccagacgttc ctctgcgggt tcatcttcac gccagagtac
    18361 aagtaatttc attgccttag cccccatttc acgggcttta tgcgggtcga cggacatatc
    18421 tatttcaacg ctatcaaccg ggatgccatt acctgggata aaaagatcgg cagcgacaat
    18481 caggccgcaa gaattagcaa cagcgccctg ctcgactatt tgctcaagac aaaattgttt
    18541 atccgccaga acggcagaag cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc
    18601 ggtgagtaca ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat
    18661 ggcctcacgc tgatcgacag ctaacatggc aaatccacca cctgggcggg agatatcttt
    18721 cagcgtaaat ggggtgaagt gggatgacat agaaactcct gttaatagat aaaattaaag
    18781 accagcctgc tggcgaacct gatttagcag ggcactccag tcctgccgcc ctttgccgtc
    18841 ttgacttgct gcagcgtaat aactttctgc tgcaatcccc atattgagcg gtgcccccgt
    18901 ctcccgagcg acgtcaactg caatacgcag atctttcaac gccagatcca ccatgaaggc
    18961 gggtgaaaga tcgcctttca gcactttgca aggccaggtg gtcgtaaaat gtcctttccc
    19021 tgctgccgta ccactcatca ccttaattgc gacatcaaga tttaatccca gtgactcaca
    19081 taacacagcg gcctcggagg acagcgcatt aagcgcaatg ctcatatagt tgttaattag
    19141 cttcacgcgg atccccattc ctgggccgcc agcctcgacc agctcattac ccatgcacat
    19201 gagaatcggg cgtgcacgtg caatctgctc atcagtgcca cccgctaaaa tcagcaatgt
    19261 gccatcaatc gcattcacgg acgttctgcc cacgggggcg tccatcatac tgatgccttt
    19321 ttcttgtaac tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg acatatcaat
    19381 caataacgcg tcagatgaaa cagcctcaca gacgcctttt tcccccaaca gaacctgtcg
    19441 aacaatgtca ccattgggaa gcatagtgat gataaattca gcgcctgctg ccgcttccgc
    19501 tggggtttgt gccgcagtgg ccccctgcgt aactagcgca tcgacggcct gtgcattgac
    19561 atcaaatacc tgcaatgaat gccctttttg caatagattg ttagccattg ggctacccat
    19621 ctgccccagc ccgagaaatg caatggttgc catgaatact ctcctgtctg acattgacaa
    19681 aataatgaca attgaaaatt cgttttcgtc tgtttttgag catatttgta atttatgttc
    19741 aaaaacacga cacgcatcac taatttgatc atttgtgtca tttaaattga ccgaagacga
    19801 caaaattaaa caggacaaga caaatatgac caaaattgca tgcgtgggta tcacagttca
    19861 ggacaggatt tattctttgc tcactctgcc ggatggcggc ggaaaatatc aggaaaatca
    19921 ttatcttgag attggtggcg gcccggcagc aacagccgct gttgcaatcg caaagcttgg
    19981 cgtagaggtt gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc
    20041 agaacttgag ggttggggcg tcaataccgc attttgccgc cgatatccta acgcccgttc
    20101 atcgcaatca gcgatcctcg ttgatcagca tggcgaacgg attatcgtta attatccaag
    20161 tcccgatctc ggtactgatg ccgaatggtt agaagcgatc gatttctctc gttatgatct
    20221 gattctggcc gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc tagcgcgtct
    20281 tgcgggtgtg acaacgcttt tagacgccga tatgacaccg caggatattt ctccacttgt
    20341 cgcgcttgcc gatcacgccg tgttctcaac accagggctg aaacgcatga ctggcctgca
    20401 gtcacctgaa gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag tgtatgttac
    20461 gctgggttct gaaggatcgt tgtggattga agatggtcat ttgtgtcaac aggaggcatt
    20521 ttctgtcaat gtggttgata ccactggagc aggcgatgtt tttcacggcg cactcgcagt
    20581 agcgttggca gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg cggttgccgc
    20641 gatgaaatgt acccagcctg gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc
    20701 atttttgtca ctttatgcgt aaaatggtaa acatctaccg aggaggggca agggagtatt
    20761 catgagtatc atcgaagtga caggtaatcc acgccatgac caactcgttc atctaatcgc
    20821 agaacgcggt tatatgaata ttgaagaact ggcacaactg ctggatgttt caacgcaaac
    20881 cgtgcgccgg gatatccgaa aactcagtga gcagggcttg atcacccgtc atcatggagg
    20941 ggccgggcgc gtctccagcg tcatgaatac cgcttttgag caacgggaac tttcgctcac
    21001 cgccgagaaa cgggcgatcg ctgaggcagt cgccgattac cttcccgaac gctgtaccgt
    21061 ctttatcacc atcggtacaa ccgtagaagc cgttgccagg gcattactca accggcgtga
    21121 tttacgcatt atcactaaca gcctacgtgt ggcacagatt ctttataaga atcaggatat
    21181 tgaagtgatg gtgccgggag gcactttacg cgctcataac ggcgggatta tcggcccagg
    21241 agccgtggat tttattgaag gcttccgcgc agattattta atcaccagta taggtgccat
    21301 tgaacatgac ggcaccctac tggaatttga tgtaaatgaa gcgttagtcg cgagaacgat
    21361 gattaaacat gcgcggaata cattgttagt ggccgatcat acgaagtttg ccgcgtctgc
    21421 tgccgtttca attggcaatg cacggaatgt cagggctttt tttacagatg ccccgcctcc
    21481 caattctttc tgccagttgt taagtgaaga gaatgttgaa ctggtggttg ccgagcagga
    21541 agtttcctga aattgaaact tcgaaaaatc cgctggggat tcagcggatt ttattttgcc
    21601 ggatgcggcc tacggttgat acggttttcg ttggccggat taagcacatc cggccattaa
    21661 ttaagcttct gccgccagca tctctttctt acgttggcta atacgcttaa ccagcattac
    21721 ggaaaccacc gcaccacaga atgcgaagca ggccataatg ccaaacacga ttttgtagcc
    21781 aataatcccc ggatttaaat ccagaatgta gccatacaga ctgaagcaga acatcgccgg
    21841 agcgtaacca ataaagctac ccaacgccat cgccgcgcct gttttatttt cagcaatttt
    21901 tgcttcgccg ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc
    21961 cagcgtacat gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag
    22021 tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag
    22081 gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt
    22141 aatgatgccg taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg
    22201 aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttaa aagcgataag
    22261 ccagatggtt ttgtctctca gcaccgaggt catactggtg ttttgcgatg ctccgtcttc
    22321 ttttttcacc tcaacggacg gtgcctcttc tttgtcattc aggacaaaga aaataataat
    22381 gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac ccaataagcc
    22441 actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc
    22501 acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact
    22561 taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca
    22621 gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt
    22681 cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga
    22741 cagaaaaaaa cccactgtcg tgacaaatga gtttaccgac atagcattac caatttgacc
    22801 attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa
    22861 tttataaatt gtaccgccac acaatgttaa tagactaaat aacgcccatt tctttttcgt
    22921 gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat aagataccga
    22981 ccattcatat ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga
    23041 agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta
    23101 attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc tggcaatggt
    23161 gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac
    23221 gccggcgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca
    23281 tcacccaggc atataaggtg cgttaataac gctttgaacg gcatatcacc ctgacctgaa
    23341 atacaggctt tatgacccag gccacccggt tctttaacga tcaaggcatc tttgatatgg
    23401 acctgggtaa tatgcggcgc catcgttttt aaagcgtcga tgggatgctc atttgcattg
    23461 atcatgttcg caaaatcaaa cagtaaggaa agagattcca tctcactttc tttgaccagc
    23521 gacaccagct catggctttt taaatcttca tgctgttcga tcgtaaaagt caggccgctg
    23581 tcctgatacg tttcccgtac ataggcaatg tcgttagcga tgatcgataa tacgtcgcgc
    23641 aaattacctt cataacgtgg ataaaaacgt acggacgatg ccccagtttt caacgcaatg
    23701 gcgacggctt cgtcaataga tgccttatct gaggcgctgg tttcaatatg aatatcaagg
    23761 ttcagacggc gggctttatc tccaaaggca gagagttctt tatcgtccat attaccaaga
    23821 gaaaaacgct cgccatccag aacatggatt ttgacgccac gtagattatt ttcacttgcg
    23881 atatcaagta aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa
    23941 gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt
    24001 gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc
    24061 aatatctatc tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc
    24121 tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt
    24181 gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa
    24241 tgagtggttt tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc
    24301 gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt
    24361 tgatggtgtc actgcaatca gtgtcgcgag cgtctcggga atatcgctgg tacggttttt
    24421 ctcggtaaac ccccaggaag gctctcgtcc ttgctcaatt gcggcccgct gaaagctcgc
    24481 cgacaactcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct tacgatcctt
    24541 acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg tcatacgttt
    24601 aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg gcgtggtgat
    24661 gccatctttt aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgatttcc
    24721 cgtcattgtt taatcctttt ggtttaaacc agatgaggca tcttacgctt acgccaaaaa
    24781 agaaagcaat taacggcgta tgactttgca gataattgtg acaaagctct ctattgacgt
    24841 aatcccagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag gtaaaaaaaa
    24901 acctgccagc gatggcaggt tttttataac taaaaaacga aattaatcgt ctttcggtgc
    24961 gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt
    25021 cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat
    25081 gcggataggc ggaaccagaa caacggcttc gtcagtaccg gaagcacgca tgttggtcag
    25141 tttcttaccg gtcaggcagt ttacagtcag gtcgttagag cggctatgaa taccgataat
    25201 ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc
    25261 gaacagcgcg aacgcgaccg ctttaccctg accgttagag atcagtacgc cgttctgacg
    25321 ctgacccact tcacccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc
    25381 agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat
    25441 cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt
    25501 acgttcgccc agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt ccagagtcac
    25561 gttttcatat ggctcttgtt tacgaccgtc gatttcacgg aagataactt tcggacggga
    25621 taccgccagt tcgaaacctt cacgacgcat gttttcgatc agaacagaca ggtgtagttc
    25681 gccacgacca gaaacgcgga acgcatcagc gtcttcggtt tcttctacgc gcagcgcaac
    25741 gttgtgtacc agttctttgt tcagacgatc caggatctga cgagacgtta cgaacttacc
    25801 ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc
    25861 aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt
    25921 cagttcgcca aggcccgtga tcgcaacgat atcgccagct tccgccaggt cggtttcgat
    25981 acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttgcggg ttttgccttc
    26041 gctatcgatg atagtgacct gctggttcgg cttcacttta ccgcgcttga tgcggccaat
    26101 gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc
    26161 gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctggtaca gcggggtcat
    26221 gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac
    26281 gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg
    26341 atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg
    26401 cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc
    26461 aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt caacttcacc
    26521 accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat
    26581 agcggtgttt ttcgcgagga tggtaatccc acgctctttc tccaaatcgt tggagtccat
    26641 cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc
    26701 taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc
    26761 gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac
    26821 tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg
    26881 ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaacatgg tgcttaatgt
    26941 ttccattgaa gcactatatt ggtgcaacat tcacatcgtg gtgcagccct tttgcacgat
    27001 ggtgcgcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc
    27061 ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg accatgacca
    27121 atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa
    27181 gtgaagtttg ttgatttgcg cttcaccgat actaaaggta aagaacagca cgtcactatc
    27241 cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg
    27301 attggcggct ggaaaggcat taacgagtcc gacatggtgc tgatgccaga cgcatccacc
    27361 gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa
    27421 cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg cgccgaagat
    27481 tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc
    27541 ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat
    27601 atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg
    27661 gcagtgaaag gcggttactt cccggttcca ccggtagact cggctcagga tattcgttct
    27721 gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta
    27781 gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac
    27841 gaaattcaga tctacaaata tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg
    27901 acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct
    27961 ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag
    28021 gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac
    28081 ccgaccacca actcttataa gcgtctggtc ccgggctatg aagcaccggt aatgctggct
    28141 tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca
    28201 cgtcgtatcg aagtacgttt cccggatccg gcagctaacc cgtacctgtg ctttgctgcc
    28261 ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac
    28321 aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct
    28381 ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg
    28441 ttcactgacg aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg
    28501 cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg
    28561 tactacccgg cgcaacaacg ccgggattta gttgccgtgg aaatttcagc ccatctctgc
    28621 atgggctttt ttctccgtca attctctgat gcttcgcgct ttttatccgt aaaaagctat
    28681 aatgcactaa aatggtgcaa cctgttcagg agactgcttt atggcaacag gcacgcagcc
    28741 cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaatcg atgacaacct
    28801 ggcgatccat tacgccaacc ctgccgcgca acaactgctc gcccaaagct cccgcaaatt
    28861 gtttggtaca ccgttaccgg aactgttgag ctacttctca ttaaatatcg agctgatgca
    28921 agaaagtctg gaggcggggc aaggttttac cgataacgaa gtgacgctgg tcatcgacgg
    28981 gcgctcgcat atcctttctg tgacggccca gcgtatgccg gacggcatga tcctgctgga
    29041 gatggctccg atggataacc agcgccgctt aagtcaggaa cagctacagc acgcccagca
    29101 ggttgctgcc cgtgatttag tgcgcggcct ggcacatgag attaaaaatc cgcttggcgg
    29161 tttacgtggc gcggcgcagc tgctcagcaa agcgttacct gacccatcac tactcgaata
    29221 taccaaagtg attatcgaac aggcggaccg gctgcgaaat ctggtcgacc gtctgttggg
    29281 gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtggctg aacgcgtggt
    29341 aacgctggtg tcgatggaac tgccggacaa cgtgcggttg attcgtgatt acgatcccag
    29401 cctgccggaa ctggcgcacg acccggatca aattgaacag gtcttgctga atattgtgcg
    29461 caatgcgcta caggcgctgg ggccggaagg cggtgaaatc attctgcgta cccgcaccgc
    29521 gtttcaactg accttacacg gcgagcgcta ccggctggcg gcgcggattg atgtggaaga
    29581 taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga tggtcagcgg
    29641 ccgcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc
    29701 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat
    29761 caggaaataa aggtgacgtt tatgcaacga gggatagtct gggtagtcga tgacgatagt
    29821 tccatccgtt gggtgcttga acgtgcgctc gctggggcag gtttaacctg tacgacgttt
    29881 gagaacggcg cagaagtgct ggaggcgctg gcgagcaaaa cgccggatgt gctgctttca
    29941 gatatccgta tgccgggaat ggacgggctg gcgctgctca agcagattaa acagcgccat
    30001 ccaatgcttc cggtcatcat tatgaccgca cattccgatc tggatgctgc cgtcagcgcc
    30061 tatcaacaag gggcgtttga ttatctgccc aaaccgtttg atatcgacga agcagtggcg
    30121 ctggttgagc gcgctatcag tcattaccag gaacagcagc agccgcgtaa tgttcagctt
    30181 aacggcccaa cgaccgatat catcggcgaa gcgccagcca tgcaggacgt gttccgtatt
    30241 atcggtcggc tttcgcgttc ttctattagc gtgctgatta acggcgaatc cggcaccggt
    30301 aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc gcgccaaagc gccgtttatc
    30361 gcgctgaata tggcagctat cccaaaagat ttgatcgaat cagaactgtt tggccacgag
    30421 aaaggcgcgt ttactggcgc gaataccatt cgtcaggggc gttttgaaca ggccgatggc
    30481 ggtacattat tcctcgacga aattggtgat atgccgctgg atgtgcagac gcgtttgctg
    30541 cgcgtgctgg cagacggtca gttttaccgc gttggcggct atgcgccggt gaaagtggat
    30601 gtgcggatta tcgctgccac tcaccagaat ctcgaacagc gagtgcagga aggtaagttc
    30661 cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc atctgccgcc gctgcgcgaa
    30721 cgtcgggaag atattccccg tctggcgcgc cattttttac aggttgccgc gcgcgaactg
    30781 ggcgtagaag cgaagttact gcatccggaa accgaagctg ctctgacgcg tctggcgtgg
    30841 ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc taacggtgat ggccgccggg
    30901 caggaagtgt tgattcagga tttgcccggc gaactgtttg aatcaacggt tgcggagagt
    30961 acttcgcaaa tgcaaccgga cagttgggcg acgcttcttg cgcagtgggc agacagagcg
    31021 ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga gcggacgtta
    31081 ctgacgaccg cgttgcgaca tacgcagggg cataaacagg aagcggcgcg gctactcggc
    31141 tggggccgca acactctgac gcgtaagtta aaagagctgg ggatggagtg attcacggcg
    31201 tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat tttgcgcttt actgttccga
    31261 taagttcagt atgatcttgc ccggaaaacg gggagagtca ttatgctgga atcaataatt
    31321 aatctggtat cgagtggcgc agttgacagc cacacaccgc aaactgccgt tgctgccgtg
    31381 ctgtgtgccg caatgattgg gctgtttagt tgagtttcag attgctgatg aagtgctctt
    31441 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaagcaat attttcaaca
    31501 ggttttgtag gcctgataag cgtagcgcat caggcaatct tgttctcagc acattgatgg
    31561 cgctttcgtt ttctactttg taaacgaagc gccattcact atttaaatca cccgtgagaa
    31621 ctgctgcatc cgcgctttct ggcgcagata ggtatcaaag cacatgcaaa tgttgcggat
    31681 cagcaagcga cctttcgccg tcacctgtat tcccttctca tccacatcca ccagcccatc
    31741 ttttgctaac ggggcgagca gcttgagatc ttccgcaaag taatcagcga agtgcaaatc
    31801 ccactgtttc tcaatagggg cgtaatccag acggaagttg cagatgagcg acttaatcac
    31861 atcgcggcga atacagtcat cacgcgttag cgcaatacca cgccacagcg cattgccttg
    31921 ttcatccact tgctgatagt actgcttcaa ctctttctgg ttctgcgcgt agcagtcgcc
    31981 aatcatgctg atggcggaaa cgcccatccc cagcagatcg gtatcgccct gagtggtgta
    32041 gccctggaag ttacgatgca gcacgccttc acgctgggcc accgccagct cgtcatccgg
    32101 acgggcaaag tgatccatac cgataaactg atagcccgat tgcgtcagga aggcgatggt
    32161 ttcctgcagg atatcgagtt tttgctgcgg actcggcagg tcagcatctt tgattttgcg
    32221 ctgagcagca aaaatggtcg gcagatgcgc gtagttaaag acactcagac gatcggggtt
    32281 cagctccgcc acacgtttca gggtaaaggc gaaactctcc ggcgtctgtt tcggcaggcc
    32341 gtaaatcagg tcgatgttgg tggaggtaaa accaatctca cgcgcatggt taagcagtgc
    32401 aaagatgaac tcttcatcct gctcgcggtt aaccagacgt tgcacttctt tgttgaagtc
    32461 ctgcacgccc atgctcaggc gattaaagcc ttcggcgcgt aaatgatcga gtacatccag
    32521 ttcgatttcc cgcggatcga cttcgatcga aatctccgca tcggcattga actggaagtt
    32581 ttcgcgcagc agcttcatca ggcggctgat ttgcgcttta ttcagatacg tcggcgttcc
    32641 gccgccccag tgcaattggc tgacgtgacg cccggcgaac agcggtgcac gatggacgat
    32701 ttcttgctcc agcgcgtcca gatactgatc ggccttgtgc tgctggcgag taacaatctt
    32761 attgcaaccg cagaagtaac aaagcttatg gcagaacggg atatgtacgt agagagataa
    32821 tggacgctca ggatagcgcg ccacggcttg taaaaacgcc tgttcgccga agtcttctga
    32881 aaactccagc gcggtcgggt acgaggtgta tcgtggcccg gaatagttat atttctggat
    32941 cagggccaga tcccagtcga tttgctgtac agacatgctc actccttccg atggcgtctc
    33001 tggcggctac ggcgtaccgg cggatgcccg tttcgggtca tcagccggtt gcgcagccac
    33061 ttttgtcgcc gcgacaaccg ctgtagttta acgaataacc acaccagata acatataacc
    33121 ggaagggtta tcagcagaac gggaaggccc acggcggcaa tccgttagtt gccccgcagc
    33181 agacgcatca tgtcttcttg cttctcgtct tcttcctctt cttcgtcatc gtcataagag
    33241 aggccgagtt tctgcatcaa ctcatcaata cgatccagtt tagcatccac ccaggattgc
    33301 tcttcggcgc tcagggtttc gcctgcttcc agacgttcca gcagcgcatc cagacgctca
    33361 tccgtttcca gtaattccaa ctccgcctgc ggtgaaagca taggtttctc actcttcggt
    33421 ttgtgctgtt tggtgacttt ttcagtcacg cccaatggaa taggggtttt actgccaata
    33481 cgtggatctt ttggtgcgtt ctggcctttg ctgcctgacg tggtgtttcc gcccgctgcg
    33541 cggctgcccg gcgcatgacc acgacgtttt ttctgacgct tgcgatcacg agcttcctga
    33601 tccagctcct cgcgtgtttt tcgacgcgct tttgcatgac ctttgctgcg tgagtttgaa
    33661 gatgatggtt tcataatggt tcgtctttta gccgttttat tcagtataga tttgcggcgg
    33721 aatctaacag aaagcaagca aagaaaaaag gcgacagatt actctgtcgc cttttttcct
    33781 gactcataac ccttaacggg tcttacaatc cttgcaggcc gccaacacac cctgtttttc
    33841 ctttagccgg aacaatccgt gttgattcca tttccgttta attacgtctc cggacgtttg
    33901 tcttcctgac aatcctctgt cttcgcctcc tggcgctcct gaccctcatc ctgagtcatt
    33961 gttcctgtta gcgtcctcgc tctatgggtg ctactttacg cctttgcttt aaacaaacaa
    34021 tcaaccgcca tcagtatttg cacattttca gagttttata taaaagcaat tcactgattt
    34081 atatagctta tttgacatgc atcgcagaat tttctctgct atttcgcata aagactatcg
    34141 gcaatgtctc acaaagcgta tggcttttac ttacaacgct cacgccgaaa aatccgcctt
    34201 ttattatggt tcaggtataa tcgccacaaa gctttgactg acggagacga ccgctttgac
    34261 taatttgaat tatcaacaga cgcattttgt gatgagtgcg cctgatattc gccacctacc
    34321 ttccgatacc ggaattgaag tggcttttgc aggccgttcc aacgcaggta aatccagcgc
    34381 gctgaacacg ctgactaacc agaaaagcct ggctcgtacc tcaaaaaccc cagggcgcac
    34441 ccagcttatc aacctgtttg aagtggctga cggcaagcgt ctggttgact tgcctgggta
    34501 cggttatgcg gaagtcccgg aagagatgaa gcgcaaatgg cagcgtgcgc tcggcgaata
    34561 cctcgaaaaa cgtcagagcc tgcaaggtct ggtggtgcta atggatattc gccatccgct
    34621 gaaagatttg gatcagcaga tgattgagtg ggcggtagac agcaatatcg ccgttctggt
    34681 gctgctgacc aaagcggaca aactggcaag cggcgcacgt aaagcgcaat tgaatatggt
    34741 gcgtgaagct gtactggcgt ttaacggtga tgtgcaggtt gaaacgtttt cttcgttgaa
    34801 gaaacaaggc gtggacaagc tgcggcagaa actggatacc tggtttagcg agatgcagcc
    34861 tgtagaagaa acgcaggacg gcgaataatt ttcttgcctt aatgcttgtg ccggatgtgg
    34921 cgtatccggc ccgtaaattc aatagattac aattctaccg ttggcctgat aaacatagcg
    34981 catcaggcat tacggatctt ttctttcgcc caataaaaaa cgccccagtc attactgact
    35041 ggggcggcta aaatattcag ccaaatccga ttacgtgaag taaaaggtct gaaagataga
    35101 acatcttacc tctgtaccct acgcgactaa ctttactctt ttttgttcag ttcagaaagc
    35161 actttttgta attttttttc agttgttgca taggaatttc taatagccat cacaaaacgt
    35221 gacagcttat gttgcttact tacgaaaaaa gggcatgttc aggcgaatct tagtgcgcct
    35281 gatcccagtt ttcgccactc cccacttcca ccagcaacgg cacatccaga cgggtacagt
    35341 tttccatcag ttgatgaatc tgcttcgcga cggcatcaac atcatcttta tgaacttcaa
    35401 ataccagttc atcgtgtacc tgcatgatca tacgtacacg cggttgctca gcctgtaacc
    35461 acgcatcaac ggcaatcatc gcccgtttga taatgtcggc ggcggttccc tgcattggcg
    35521 cgttaatggc tgcacgttca gccgctgcac gacgagcacc attgctggat ttgatatccg
    35581 gcagatacag acggcgtccg tccagcgttt caacgtagcc ctgctctttc gcctgagcac
    35641 gggtgcgttc catatactcc agcacgccag ggtagcgttc gaagtaaagg tccatgtact
    35701 tctgcgcttc tttacgtgga atgttcaatt gccgcgccag accgaaagca ctcatgccat
    35761 aaatcagacc aaagttgatc gctttcgcgc tacggcgttg ctcgctggtg acggtttcca
    35821 gtggcaaacc aaacacttct gccgccgttg cccggtggat atcttttcct tccgcgaatg
    35881 cggtcagcaa gcctttgtca cgcgagagat gcgccataat gcgcagttca atctgcgagt
    35941 agtccgctga gacaatcaca taatcctctg gcgcaataaa cgcctggcgg atacgacgac
    36001 cttcttcgtt acgcaccgga atgttttgca ggttaggatc ggttgacgat aaacgtcccg
    36061 ttgcagttac tgcctggtga taagaggtat gcacacgccc ggttttcggg ttgatcatca
    36121 gcggcagctt gtcggtgtag gtcgatttca gcttcgccag accacgatac tccagaatca
    36181 cttttggcaa cggatagtcc agcgccagtt cttccagtac ctcttccgac gttgacggcg
    36241 cgccacccgg cgttttcttc agcggtttaa taccctgttt ttcaaacaga atggtttgca
    36301 actgcttggt ggaagaaagg ttaaattctt cacctgcaat ttcatgcgct ttcttttcca
    36361 gctcagccag acgcaaggtg agctcttcag aatgattgtg cagcactttc ggatcgatct
    36421 tcacaccgtt acgttcaatg cgtgaaagca ccggcaccag cggcatttcg atattctcga
    36481 agacgttcaa cggccctttg tgtttttgca ggtccggcca cattttcaga tgcaactgca
    36541 aggtgacatc agcatcttcg gcggcgtaac gtccggcctc ttcgagggca atctggttaa
    36601 aggtcagttg atttttgcct ttacccgcaa tctcttcaaa agtgatggtt ttgtgcttca
    36661 accaacgttc cgcgaggctg tccatatcgt gacgcccggc aacgctattg agaatgtagg
    36721 actccagcat ggtatcaaac gcaatcccac gcagctcaat gccgtaattc gccagaatac
    36781 cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt ttcatcttcc agcagcggtt
    36841 ttagcaactc gagtgcacgc tcgcgagaga tttgatcggg cgcatcaaga taatcatgag
    36901 caaccggaat atatgccgct acgcctggct cgatagcaaa agaaagcccg accaggttag
    36961 cagagatgtt atcaaggctg tcggtttcgg tatcaaatgc aaataccggc gctttttcca
    37021 gcttcgcaat ccacgctttc agtgtttctt catcaaggat ggtgacgtag ttgtcataag
    37081 aaatcaccgt tgccgtcact tctggcgctt cgtctgcaac actggtttcc tgcggcttcg
    37141 cggctggttt tgcccctttg gcctgtaacc atttgcccgc ttcgacatca gcagtccagc
    37201 gtttgaactc atactttttg aacagcccca acaactcttc cgctgccggt tgctgcactt
    37261 ccagttgttc acaggtcagc tccagttcaa cgtcggtttt aatcgtcgcc agctggtatg
    37321 agagataagc aacttctttg ttttgctcga gcttcgctgc cattgttttc gcgccacgga
    37381 agctcaaccc agcaattttt tctggctcgg catacagcgt atccagcccg cccagacctt
    37441 gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac gccagggatg ttatcggagg
    37501 agtcacccat cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca
    37561 ccacctcttc cggtccgagg atggtattcg tcatggtatt gataagcgta atatttggcg
    37621 tcaccagctg cgccatatct ttatcgccag tgctgatcag caccggacgc ccggcttttt
    37681 cggcttcgcg cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca
    37741 gcggcagtcc catcgcttta accatcgcgt gcaagggttc gatttgtgca cgcagatcgt
    37801 ccggcattgg cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc
    37861 ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca
    37921 gcatgttgag gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg
    37981 gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt
    38041 gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca
    38101 gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg
    38161 gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa
    38221 tttattatca tgctttttac agcatgatgt ttgtctttgt ttttcatgca gttaaaaata
    38281 aatccatccc taataattga gataatggga ttttttctaa tgtggataga tatgaattat
    38341 ttttctcctt aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg
    38401 cttctttagc gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact
    38461 cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc
    38521 cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg
    38581 aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt
    38641 actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa
    38701 gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt
    38761 gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcaacagc tggcctgggt
    38821 gccatttctt ggcctggcgt gctggtcgct ggatatgccg tttatgaagc gttattcccg
    38881 cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc
    38941 ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat ttcgttgaag gctcccgttt
    39001 cacgcaagaa aaacatcagc aaacccactc cacttttcaa aacttgttgc caccaaaggc
    39061 ggcaggcatt gcgatggcgc taaatgtact gggcaaacaa ttcgataaac tgcttaatgt
    39121 tacactgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
    39181 gacgcggatt gtcgtacatg tggatttaca gcctattgcc gatgagttac atggcgacta
    39241 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcat tgtggcagga
    39301 aaaagaccgg cttctgacgt ctctcatgtc atcacagcgt cagaataagt aagttttact
    39361 cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa caaaacgtcc ggcacgagac
    39421 ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa ctttacccgg tgtataacgc
    39481 agctgcagcg aggcggtatt gttgtcaatc gtgattaact gaaacatctg acccaatgtc
    39541 atcatcccgc tgatttgttt atcagcctgc ttttgcgctt tttccgcatc cattccttca
    39601 gataaattaa gctgttttgc ggtttctgtg acgacattaa gcggtaactt cacatcaaaa
    39661 ttgagcgatt tgatatcttt gtttgtggat gatgaagatt tggctgggtc ggcaatactg
    39721 atatccagat tggcatttag ttcgccgagt gcgttcttcc atcttaccgg ttgtttaatg
    39781 gtcggctcac ttttttgtaa taacggtaaa tactctttga acaatgcagc attcacttct
    39841 tgcagagcaa cttcatcgtt tgctaactca gggtgtacag caagctgctt ctgcatagca
    39901 atgttatact ggatgataaa ttggcgcact gctgagggat caatagattc agcaatcaat
    39961 gaccactcac cactaccgag ttgctgattc ccttttgtta acttatcaag ttcgtaggtc
    40021 agattgacat tgatataatc tttgtcttta acgcgatcca gcatggtttt tgcaacgaaa
    40081 gcatcgattt gggcaacgtc ttccccgtga ttgataatgt taatctgatt aaatttcgac
    40141 tcgctttcac caaccggaaa tttcttttct tctaatcgcg ccagagaatc aatggtaaaa
    40201 ctttttgccg ttactttgtt cattggtgat aattgcagaa caatatcttc gactttgcct
    40261 tcaatatcca ggttagacaa tgaactacca tcaccattaa aagtaaactg gccctcacca
    40321 aaagaaaact ttccatactc tgcaggggca acatctaatg tcgtagagaa tgagccacca
    40381 aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt tcgcggcgat aaatagcggt
    40441 tgtgtcagtt cattattcac taagttcact tttgccgctg ccagtgctgg gatgacatta
    40501 ccatgcatta acattgtgat gggcaacgga ccgtgctcaa catccacatc aaatacaact
    40561 ttctggcctg gcttgatatt gagatcgggt gcaccgttat cgaatgtcat ttgcatttga
    40621 aaacgggaat taaaaaagcc tttgtcaaaa ttttgataac ttaacgtcat atcgtaggca
    40681 tgttcacctt tctttttagc atcgttaaaa tctttaataa atttttcgac accaggctga
    40741 atttgtgtac ctgtgtacca tgtgccacca ccccagatta cggctaacgc aacaataaca
    40801 cctgtagctg acttacgtat catataaata ttccctgata aaacagcaaa actttgaata
    40861 tccacttatg ctgaaataat gt
//
LOCUS       DAZFVO020000024        38831 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFVO020000024 DAZFVO020000000
VERSION     DAZFVO020000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 38831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.024"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHKNB_RS05980"
                     /old_locus_tag="pgaptmp_001196"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHKNB_RS05980"
                     /old_locus_tag="pgaptmp_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4194751.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1477)
                     /locus_tag="ACHKNB_RS05985"
                     /old_locus_tag="pgaptmp_001197"
     CDS             complement(1358..1477)
                     /locus_tag="ACHKNB_RS05985"
                     /old_locus_tag="pgaptmp_001197"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HGU4194752.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            1733..2041
                     /gene="asr"
                     /locus_tag="ACHKNB_RS05990"
                     /old_locus_tag="pgaptmp_001198"
     CDS             1733..2041
                     /gene="asr"
                     /locus_tag="ACHKNB_RS05990"
                     /old_locus_tag="pgaptmp_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HGU4194753.1"
                     /translation="MKKVLGLVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            2134..2217
                     /locus_tag="ACHKNB_RS05995"
                     /old_locus_tag="pgaptmp_001199"
     CDS             2134..2217
                     /locus_tag="ACHKNB_RS05995"
                     /old_locus_tag="pgaptmp_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194754.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHKNB_RS06000"
                     /old_locus_tag="pgaptmp_001200"
     CDS             2317..3138
                     /gene="ydgD"
                     /locus_tag="ACHKNB_RS06000"
                     /old_locus_tag="pgaptmp_001200"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HGU4194755.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3228..3506)
                     /gene="mdtI"
                     /locus_tag="ACHKNB_RS06005"
                     /old_locus_tag="pgaptmp_001201"
     CDS             complement(3228..3506)
                     /gene="mdtI"
                     /locus_tag="ACHKNB_RS06005"
                     /old_locus_tag="pgaptmp_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HGU4194756.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKG"
     gene            complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHKNB_RS06010"
                     /old_locus_tag="pgaptmp_001202"
     CDS             complement(3493..3858)
                     /gene="mdtJ"
                     /locus_tag="ACHKNB_RS06010"
                     /old_locus_tag="pgaptmp_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HGU4194757.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHKNB_RS06015"
                     /old_locus_tag="pgaptmp_001203"
     CDS             4270..5304
                     /gene="tqsA"
                     /locus_tag="ACHKNB_RS06015"
                     /old_locus_tag="pgaptmp_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HGU4194758.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHKNB_RS06020"
                     /old_locus_tag="pgaptmp_001204"
     CDS             complement(5329..6717)
                     /gene="pntB"
                     /locus_tag="ACHKNB_RS06020"
                     /old_locus_tag="pgaptmp_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HGU4194759.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTQMLFGD
                     AKASVDAILKAL"
     gene            complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHKNB_RS06025"
                     /old_locus_tag="pgaptmp_001205"
     CDS             complement(6728..8260)
                     /gene="pntA"
                     /locus_tag="ACHKNB_RS06025"
                     /old_locus_tag="pgaptmp_001205"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HGU4194760.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHKNB_RS06030"
                     /old_locus_tag="pgaptmp_001206"
     CDS             8784..9728
                     /gene="ydgH"
                     /locus_tag="ACHKNB_RS06030"
                     /old_locus_tag="pgaptmp_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HGU4194761.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHKNB_RS06035"
                     /old_locus_tag="pgaptmp_001207"
     CDS             9914..11296
                     /gene="ydgI"
                     /locus_tag="ACHKNB_RS06035"
                     /old_locus_tag="pgaptmp_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4194762.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11333..12055
                     /gene="folM"
                     /locus_tag="ACHKNB_RS06040"
                     /old_locus_tag="pgaptmp_001208"
     CDS             11333..12055
                     /gene="folM"
                     /locus_tag="ACHKNB_RS06040"
                     /old_locus_tag="pgaptmp_001208"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HGU4194763.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHKNB_RS06045"
                     /old_locus_tag="pgaptmp_001209"
     CDS             complement(12052..12387)
                     /gene="ydgC"
                     /locus_tag="ACHKNB_RS06045"
                     /old_locus_tag="pgaptmp_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HGU4194764.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12516..13235
                     /gene="rstA"
                     /locus_tag="ACHKNB_RS06050"
                     /old_locus_tag="pgaptmp_001210"
     CDS             12516..13235
                     /gene="rstA"
                     /locus_tag="ACHKNB_RS06050"
                     /old_locus_tag="pgaptmp_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HGU4194765.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13239..14540
                     /gene="rstB"
                     /locus_tag="ACHKNB_RS06055"
                     /old_locus_tag="pgaptmp_001211"
     CDS             13239..14540
                     /gene="rstB"
                     /locus_tag="ACHKNB_RS06055"
                     /old_locus_tag="pgaptmp_001211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HGU4194766.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSP"
     gene            14616..15545
                     /gene="tus"
                     /locus_tag="ACHKNB_RS06060"
                     /old_locus_tag="pgaptmp_001212"
     CDS             14616..15545
                     /gene="tus"
                     /locus_tag="ACHKNB_RS06060"
                     /old_locus_tag="pgaptmp_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HGU4194767.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHKNB_RS06065"
                     /old_locus_tag="pgaptmp_001213"
     CDS             complement(15542..16945)
                     /gene="fumC"
                     /locus_tag="ACHKNB_RS06065"
                     /old_locus_tag="pgaptmp_001213"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HGU4194768.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGSRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHKNB_RS06070"
                     /old_locus_tag="pgaptmp_001214"
     CDS             complement(17088..18734)
                     /gene="fumB"
                     /locus_tag="ACHKNB_RS06070"
                     /old_locus_tag="pgaptmp_001214"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4194769.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAL
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            18933..20108
                     /gene="manA"
                     /locus_tag="ACHKNB_RS06075"
                     /old_locus_tag="pgaptmp_001215"
     CDS             18933..20108
                     /gene="manA"
                     /locus_tag="ACHKNB_RS06075"
                     /old_locus_tag="pgaptmp_001215"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HGU4194770.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHKNB_RS06080"
                     /old_locus_tag="pgaptmp_001216"
     CDS             20209..21717
                     /gene="ydgA"
                     /locus_tag="ACHKNB_RS06080"
                     /old_locus_tag="pgaptmp_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HGU4194771.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(21762..23027)
                     /gene="uidC"
                     /locus_tag="ACHKNB_RS06085"
                     /old_locus_tag="pgaptmp_001217"
     CDS             complement(21762..23027)
                     /gene="uidC"
                     /locus_tag="ACHKNB_RS06085"
                     /old_locus_tag="pgaptmp_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HGU4194772.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23066..24439)
                     /gene="uidB"
                     /locus_tag="ACHKNB_RS06090"
                     /old_locus_tag="pgaptmp_001218"
     CDS             complement(23066..24439)
                     /gene="uidB"
                     /locus_tag="ACHKNB_RS06090"
                     /old_locus_tag="pgaptmp_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HGU4194773.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24436..26247)
                     /gene="uidA"
                     /locus_tag="ACHKNB_RS06095"
                     /old_locus_tag="pgaptmp_001219"
     CDS             complement(24436..26247)
                     /gene="uidA"
                     /locus_tag="ACHKNB_RS06095"
                     /old_locus_tag="pgaptmp_001219"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HGU4194774.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRIFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(26636..27226)
                     /gene="uidR"
                     /locus_tag="ACHKNB_RS06100"
                     /old_locus_tag="pgaptmp_001220"
     CDS             complement(26636..27226)
                     /gene="uidR"
                     /locus_tag="ACHKNB_RS06100"
                     /old_locus_tag="pgaptmp_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HGU4194775.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27455..28222)
                     /gene="hdhA"
                     /locus_tag="ACHKNB_RS06105"
                     /old_locus_tag="pgaptmp_001221"
     CDS             complement(27455..28222)
                     /gene="hdhA"
                     /locus_tag="ACHKNB_RS06105"
                     /old_locus_tag="pgaptmp_001221"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HGU4194776.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28334..29362)
                     /gene="malI"
                     /locus_tag="ACHKNB_RS06110"
                     /old_locus_tag="pgaptmp_001222"
     CDS             complement(28334..29362)
                     /gene="malI"
                     /locus_tag="ACHKNB_RS06110"
                     /old_locus_tag="pgaptmp_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HGU4194777.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29537..31129
                     /gene="malX"
                     /locus_tag="ACHKNB_RS06115"
                     /old_locus_tag="pgaptmp_001223"
     CDS             29537..31129
                     /gene="malX"
                     /locus_tag="ACHKNB_RS06115"
                     /old_locus_tag="pgaptmp_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HGU4194778.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            31139..32311
                     /gene="malY"
                     /locus_tag="ACHKNB_RS06120"
                     /old_locus_tag="pgaptmp_001224"
     CDS             31139..32311
                     /gene="malY"
                     /locus_tag="ACHKNB_RS06120"
                     /old_locus_tag="pgaptmp_001224"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HGU4194779.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32415..33416
                     /gene="add"
                     /locus_tag="ACHKNB_RS06125"
                     /old_locus_tag="pgaptmp_001225"
     CDS             32415..33416
                     /gene="add"
                     /locus_tag="ACHKNB_RS06125"
                     /old_locus_tag="pgaptmp_001225"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HGU4194780.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPSVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(33451..34491)
                     /gene="ydgJ"
                     /locus_tag="ACHKNB_RS06130"
                     /old_locus_tag="pgaptmp_001226"
     CDS             complement(33451..34491)
                     /gene="ydgJ"
                     /locus_tag="ACHKNB_RS06130"
                     /old_locus_tag="pgaptmp_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4194781.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFAVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34734..34859
                     /gene="blr"
                     /locus_tag="ACHKNB_RS06135"
                     /old_locus_tag="pgaptmp_001227"
     CDS             34734..34859
                     /gene="blr"
                     /locus_tag="ACHKNB_RS06135"
                     /old_locus_tag="pgaptmp_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HGU4194782.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            35132..35347
                     /gene="ydgT"
                     /locus_tag="ACHKNB_RS06140"
                     /old_locus_tag="pgaptmp_001228"
     CDS             35132..35347
                     /gene="ydgT"
                     /locus_tag="ACHKNB_RS06140"
                     /old_locus_tag="pgaptmp_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HGU4194783.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            35433..35873
                     /gene="ydgK"
                     /locus_tag="ACHKNB_RS06145"
                     /old_locus_tag="pgaptmp_001229"
     CDS             35433..35873
                     /gene="ydgK"
                     /locus_tag="ACHKNB_RS06145"
                     /old_locus_tag="pgaptmp_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HGU4194784.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            35950..36531
                     /gene="rsxA"
                     /locus_tag="ACHKNB_RS06150"
                     /old_locus_tag="pgaptmp_001230"
     CDS             35950..36531
                     /gene="rsxA"
                     /locus_tag="ACHKNB_RS06150"
                     /old_locus_tag="pgaptmp_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HGU4194785.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            36531..37109
                     /gene="rsxB"
                     /locus_tag="ACHKNB_RS06155"
                     /old_locus_tag="pgaptmp_001231"
     CDS             36531..37109
                     /gene="rsxB"
                     /locus_tag="ACHKNB_RS06155"
                     /old_locus_tag="pgaptmp_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HGU4194786.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            37102..>38831
                     /gene="rsxC"
                     /locus_tag="ACHKNB_RS06160"
                     /old_locus_tag="pgaptmp_001232"
     CDS             37102..>38831
                     /gene="rsxC"
                     /locus_tag="ACHKNB_RS06160"
                     /old_locus_tag="pgaptmp_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HGU4194787.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQA"
BASE COUNT         9443 a        10024 c         9501 g         9863 t
ORIGIN      
        1 ttttcctacc ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc gggcgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaag acgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctttccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc taaagccgga accatgacca cccgctacgg
      961 gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc
     1321 ggactgcggt aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc
     1501 taccgctttg taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa
     1561 atttccctta agtcacaata cgttattatc aatgctgtat tttattcagc gtttgtacat
     1621 atcgttacac gctgaaacca accactcacg gaagtctgcc attcccaggg atatagttat
     1681 ttcaacggcc ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa
     1741 agtattaggt ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga
     1801 gactgcgacc acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaactac
     1861 acatcataaa aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca
     1921 tcataaaaat acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa
     1981 acacgccggg aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta
     2041 agttttcaac agtaatgccg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg
     2101 taacgtttag caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc
     2161 attcttatca tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg
     2221 tagttatctg attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt
     2281 gcttttaata atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg
     2341 ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa
     2401 tcggcaaacg atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat
     2461 gacacgaccc aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta
     2521 tgtacagcga cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg
     2581 ccgcctaagg gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa taaaggtctt
     2641 tggcgctatg acatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta
     2701 aaagcagatg gggatggttg gattgttcct cccgcagctg caccatggga cttcggcttg
     2761 attgtgctgc gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa
     2821 gccgcgctta ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggatatcct
     2881 gaagatcatc tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa
     2941 acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg
     3001 ttgcataccg atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa
     3061 gatcgctggc gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat
     3121 caactgtcgc aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg tgttcatcag
     3181 gcaagtttca ccatgatcat tccagccaac agcaagacca ggccaattca gcctttacga
     3241 tttaaccgtt gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg
     3301 ccccacaatg cataagcgac agacaagtcg atccctttta cggcctgaga aagcgcacta
     3361 aaggcagcca gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca
     3421 gaaaatttca aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg
     3481 gcgtgaaccc attcaaactg cgccatggtt cacctccagt tcaggtttac gcgctttacg
     3541 ggtacctgat ttgatcaaca caatcccggc gaccagggtg gttaacccgg caattttcat
     3601 cagcgataaa ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat
     3661 accttcccac agcgcataag ctacgcctaa ggcgatcttt ttaacggcga aagagagaaa
     3721 tatatacgac agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac
     3781 gctcgcccat ttcattgaca gcgtaccggt aatttctgta gcaatagcca ggcctaataa
     3841 aatccaataa atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat
     3901 tcagcgaaac caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta ggcagaagaa
     3961 aggactaaag cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac
     4021 gaagatgggt aaatgttgaa aataacgtcc tgaacaaatt gtccataata ttacaattat
     4081 ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg
     4141 atttgtatca tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt
     4201 gattttcgtt atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaatg
     4261 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
     4321 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
     4381 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
     4441 cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggcgatggtg
     4501 ttgctattag cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat
     4561 cgcaactcta ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt
     4621 gacgtctcag ttgaccagct ggcgcattac attgatccga acgcggcgat gacgttgctc
     4681 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg
     4741 gtgctgttta tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
     4801 cgtccggttg aagggatggc ggcgattcaa cgtgcaattg acagtgtttc tcattatctg
     4861 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca
     4921 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct
     4981 aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc
     5041 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
     5101 attctggagc cgcgcatcat gggacgtggg ctggggcttt ccacattggt ggtatttttg
     5161 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgtta
     5221 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
     5281 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
     5341 caggattgca tccacgctgg ctttggcgtc accaaacagc atttgggtgt tttccttgaa
     5401 gaacagcggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
     5461 gttctgcgct ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc
     5521 ctgcgccgcc gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc
     5581 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
     5641 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
     5701 gttgatacca cgagcgcgca gtttctcggt aatttcagcg acaggatatt gcgcctgcgc
     5761 gactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
     5821 tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcgc cagtagaaga
     5881 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
     5941 cataatgtaa gaaaggatag cgcccgaaga accgaccagc gcaccggtca caatcagcag
     6001 gtcgttgctg agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
     6061 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
     6121 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tatccgtgcg
     6181 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
     6241 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac
     6301 cgaacccgtg aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
     6361 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga taactgttaa agccaaccag
     6421 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
     6481 ttcaactttc ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat
     6541 ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatccc
     6601 ggcgataccg aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact
     6661 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
     6721 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
     6781 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
     6841 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
     6901 tcaacggtgt atgcagcgca tgcgatacat tccacaccac gtaataaccg acaacgcagg
     6961 ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccaac
     7021 cgaaaaggat gattgccagc gccatcaatg cgtatttacg ccacggtgag caggcacatt
     7081 tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
     7141 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
     7201 ccacatcatc aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca
     7261 gattaacgag gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
     7321 cggtataacc aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
     7381 attcacagtt gccaccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
     7441 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
     7501 tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga
     7561 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
     7621 gctcgaggaa ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
     7681 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac
     7741 ccgcaccaat caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg
     7801 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg
     7861 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gaatccatcg
     7921 ccatcacggt cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
     7981 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
     8041 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
     8101 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
     8161 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
     8221 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
     8281 ttattgatga tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
     8341 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
     8401 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
     8461 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt
     8521 cgggtaaaac gagacatcgc cccggcacga atcactactt aacattaaat taacttatac
     8581 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
     8641 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
     8701 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
     8761 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
     8821 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
     8881 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
     8941 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
     9001 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
     9061 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
     9121 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
     9181 agccagaagt caatgatgcc atcaccaaag cagcaaaagc gaaaggtgcc tactctttct
     9241 acattgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
     9301 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
     9361 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccag
     9421 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg tttctttgaa acccagtcat
     9481 caaaaggcgg acgttacact gtcacgcttc ctgacggcac taaagtcgaa gaactgaaca
     9541 aagcgacagc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     9601 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     9661 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
     9721 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
     9781 cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     9841 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     9901 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     9961 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    10021 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
    10081 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    10141 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
    10201 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    10261 gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatagtcg gtgcatcggc
    10321 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
    10381 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
    10441 gatgttcaaa ctggatactt tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
    10501 gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    10561 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    10621 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggcgtggt
    10681 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
    10741 gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
    10801 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcaa ctcataaagc
    10861 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cgctgtggtt
    10921 gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
    10981 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    11041 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    11101 tggcttatgg ttattgtatg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    11161 tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact
    11221 gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
    11281 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
    11341 agcccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
    11401 ctggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacgc actatccagc
    11461 cattgatgga ctgattaagg caggtgcgca gtgtattcag gctgattttt cgaccaacga
    11521 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
    11581 gcataacgcc agtgcgtgga tggcggaaaa accaggtaca ccactggccg acgtactggc
    11641 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
    11701 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    11761 gcgcggtagc gacaaacatg ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
    11821 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    11881 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
    11941 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    12001 ctgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
    12061 ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac
    12121 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa
    12181 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
    12241 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
    12301 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    12361 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    12421 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
    12481 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    12541 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
    12601 ccgtagaacc gcgcggcgac caggccgaag aaaccatttt gcgagaaagt ccggatttgg
    12661 tgttactcga catcatgcta ccaggcaagg atggcatgac catttgtcgt gatttacgcg
    12721 caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    12781 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc
    12841 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgacc aaaggtcttc
    12901 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca
    12961 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    13021 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    13081 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    13141 taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact gtgcgtaaca
    13201 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
    13261 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ttggtgtaca
    13321 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    13381 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
    13441 agatggattt aaatctctct ttcgatctgc gtgtcgagcc attgagtaaa taccatcttg
    13501 atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
    13561 cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    13621 tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt
    13681 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    13741 aagcagcggc gcaacgattt ggcgatgggc atctcaatga gcgtatccac tttgatgagg
    13801 gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct
    13861 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    13921 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    13981 tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    14041 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    14101 cgcatctggc agacattcag gcagtaacgc ccgataaaac ggtacggatt aaaacgctcg
    14161 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    14221 tcaataacgc cctgcggtac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
    14281 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
    14341 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    14401 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    14461 acaccagcga actgggtggt gctcgcttct cgtttagctg gccgttatgg cataacatcc
    14521 cgcaatttac ctctccctga cactacgcgc acgatggtca agtcaccacg actgtgctat
    14581 aaaataagta tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    14641 gactcaacac tacctttcgc cagatggaac aagagctggc tacatttgcc gctcatcttg
    14701 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    14761 agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc
    14821 tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    14881 aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
    14941 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    15001 cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
    15061 ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac
    15121 gctttggttg ggctaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac
    15181 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    15241 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa
    15301 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    15361 aacaagtgca acacgcctgc cctacaccgc tgattgcact gattaatcgg gataatggtg
    15421 cgggcgtgcc agacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
    15481 ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    15541 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
    15601 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    15661 gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    15721 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
    15781 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    15841 aattatggat caccatcgga cggaagacgt tcagttcaaa gttaccggaa gcgccaccca
    15901 tgttgatcgc cacgtcgttc cccattacct gacagcagag catggttaat gcttcacact
    15961 gggttgggtt cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga
    16021 tttcaccaat tccgcagcgc gagccagagg ccagccagcg gacatcattg gcgattttca
    16081 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
    16141 caagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    16201 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
    16261 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
    16321 cgagcatcgc tacccagccg gaaatctcct gccctagtgt tagcggcgtg gcgtcctgca
    16381 agtgggttcg accgattttg acgatatcgg caaatgcacg cgatttttca ctcagtgtct
    16441 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
    16501 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    16561 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
    16621 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    16681 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    16741 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    16801 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    16861 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
    16921 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgtttaattt tttctttctg
    16981 ttttgctttc gttaagcaac ttttagctca cttattattt actatttgat aacaaatgtt
    17041 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    17101 cgagtgcact gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    17161 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
    17221 tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg
    17281 atactgccaa ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    17341 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
    17401 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    17461 gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta
    17521 tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta
    17581 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    17641 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttcagggata
    17701 tatttacctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    17761 ttgatattac ggtcagcaga gcaggaaaca cccataccaa ccggacagga tgcgccgtga
    17821 cgtggcaggc gaatcacgcg tatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
    17881 agaccgagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaatgcc
    17941 tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc
    18001 actgttttaa ggttagtttc tgcagaagtt ccaccaatta cgaacgcaat atgatacgga
    18061 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
    18121 ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    18181 ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct
    18241 ggcagattgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag
    18301 cgcaaattat cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacag
    18361 ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcagtgcc ggtatcctga
    18421 caggttggca gaacgccttt cgccgcgatg tcggagttac gcaggaattg cagcgccaca
    18481 tatttatcat tttcgctggc ctccgggtca cgcagaatgt cggccacctg ttgttggtga
    18541 gccggacgca gcataaacga cgcatcatga aacgcctgac gcgccaacag agttaacgct
    18601 tcgagtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    18661 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    18721 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    18781 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    18841 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagtgg
    18901 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    18961 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    19021 gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca cgagtgcaga
    19081 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa tcgactctgc
    19141 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    19201 cagcacagcc gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg
    19261 ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta
    19321 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    19381 agttttccga gattgtctcc ctactccagc cagtcgcagg tgcacatccg gcaattgctc
    19441 actttttaca acagcctaat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    19501 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggcgcta gacagtcagc
    19561 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggaa gacagcggcc
    19621 tgttctcccc gctgttactg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    19681 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg
    19741 ataacgtgct gcgtgcgggt ctgacgccta agtacattga tattccggaa ctggttgcca
    19801 atgtgaaatt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg
    19861 cagaactgga cttcccgatt ccagtggatg attttgcctt ctcgctgcat gaccttagtg
    19921 ataaagaaac caccattagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    19981 caacgttgtg gaaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    20041 ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcgcgt gtttacaaca
    20101 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    20161 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    20221 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    20281 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    20341 aaactgacag ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    20401 ttcagcagcc agttgcaact gttggtgaaa cccattgccg ggaaagaaaa tccgtggatt
    20461 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc
    20521 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    20581 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    20641 tcgcgcattg gttacagcgg tgattccagt tccgatattt cgctcaagcc actgaattac
    20701 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgctgacaga
    20761 gacggcaaag ccatctccct ttccggggag gcgcaaagtg gtcggataga cgcagttaac
    20821 gaatataacc agaaagtgca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    20881 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    20941 tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    21001 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    21061 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca gattgatggt
    21121 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    21181 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    21241 gccctgccgc tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    21301 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    21361 aaagaagcgc cgcaaacgct ggcgcaggag gtagatcgtt cggttaaatc tctggatgcg
    21421 aaactgacca ttccggtgga tatggcaact gagtttatga ctcaggtagc gaagctggaa
    21481 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    21541 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    21601 tatactaacg gtcagataac gttaaacggg cagaaaatgc cactggaaga tttcgttggt
    21661 atgtttgcaa tgccggcatt aaatgttccg gtcgtacccg ctattccgca gcagtaattc
    21721 acagacaaaa cgccggagat ttttctctcc ggcgttattt tttacttcag cataaagtca
    21781 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg
    21841 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    21901 ctgacccgat agttcatacc acctaagata cccgtgcctt caatccgcac atctcgttta
    21961 ctggattcat agccgcgagt cacaatcggt gcaaatgtca gcgtaaattg cggcgttaaa
    22021 cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc aagacttaat
    22081 gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa
    22141 ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac
    22201 gccgtttcat tgggctggct ggtattacga cttaaacctt cgagctgcgc gtaaaagccg
    22261 agcaactccc ctttcaacgt ataattcggg tcgagcggta tgcttttctc gagaatactt
    22321 tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca
    22381 tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg
    22441 ctgttataca ccccttcatc acgaataccg gttgttgaat cagtccataa aaatactcgt
    22501 gaacgccaca ttccccacag atcaatattt ggtgttaaag cccaataacc taatgctcct
    22561 tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttagcggaat acgataaggt
    22621 aagctaccat aactataatc cgtcccgaag cgacctaatt taagcaggct attttcactg
    22681 ggttttattt ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac
    22741 cgggtactca tatcagcacg agcacctaat ttataaacat aatatgcccc gccttcaacg
    22801 ccaataatat tggaataata accactattg aaatcgagca atcctccctg tacccatgcg
    22861 taaatatctc ttcccgcgcc agcactgggc ttatccgccc tgcgcaattc gtttttcagt
    22921 ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg cataagcaga ggtaaggcca
    22981 gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg
    23041 atgttctgat tatttttatt gaatattaat tagtgatatc gctgattaat tgctgctgca
    23101 cttttttacg attatcaatt tcaaccacga tttctttgaa ttttttatcc gtgagcggat
    23161 aaaaccagat aataacgaat gccagtagca taaatccgca aggtactaag gcaattgatg
    23221 tgcggatgcc cataataact tccggcgttt gcacctgatt ggcgatatat ccgcttaatc
    23281 ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg gtaaatgaga
    23341 atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg tattctacgg
    23401 tatcagcttc cagtgcccac atcacagtca tagtaacgcc ctgaccaatt gaagcgatgg
    23461 ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaat agcagataac
    23521 cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc ctcgcgacca
    23581 tccccggcac cagcggtgcc gatgccacag taccaaccag gttttgcacc agtaccagca
    23641 cagtgaacag cccggtatca tttaacacat agcgcacgta gaacaacgac gaggcgctga
    23701 ccgcgaaggt cgaaatcagc acacacagcg caccgatgca caacataaac agcgggcgat
    23761 tccgtttcag ggtttgcaga ctgatcttca atgacggctg cgccacgata cgtaccacat
    23821 tctcacgcgt cgatttgaag cagatgaagt aaagcaccat tccggcaatc gccagcacaa
    23881 tcgtccagaa atggtatacc gacaccatct cttccgggct ggagttctta atgctcggtc
    23941 ctatcagaaa tgccaggcag acaaaggtta atgaagcggc aatcccgcga gccgcgccca
    24001 gacgggcgcg ggattgtggt tgttgggtca tcgcggtagc aagtgaacca taaggaatat
    24061 tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa atatgcatac actactttgc
    24121 tgctatggct ccagtcggtc ggtacccaga ataccagcac gctgaagatc attaacggcg
    24181 cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg ggtattcaca ctgtccacca
    24241 ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg caccagtaac agcatggtgc
    24301 ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta actcaacagg aagagcgccc
    24361 ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct gtagccgacg atggtgcgcc
    24421 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
    24481 agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat
    24541 ccctttcttg ttacctccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
    24601 ccatacctgt tcaccgacga cggcgctgac gcgatcaaag atgcggtgat acatatccag
    24661 ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca gcccggctaa
    24721 cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
    24781 aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccatcc
    24841 gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
    24901 gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc
    24961 cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
    25021 actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg
    25081 taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
    25141 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag cagtttcatc
    25201 aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
    25261 cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt gcaccatcag
    25321 cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa
    25381 gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc
    25441 gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
    25501 gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctagt
    25561 gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc
    25621 attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac
    25681 cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat
    25741 cccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt
    25801 aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat
    25861 acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc
    25921 gccctgatgc tccatcactt cctgattatt gacccacact ttgccgtaat gagtgaccgc
    25981 atcgaaacgc agcacgatac gctggcctgc ccaacctttc ggtataaaga cttcgcgctg
    26041 ataccagacg ttgcccgcat aattacgaat atctgcatcg gcgaactgat cgttaaaact
    26101 gcctggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat
    26161 tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg
    26221 ggttggggtt tctacaggac gtaacataag ggactcctca ttaagataaa aatactggtc
    26281 aacctttaat ctgattagtt aaccaattct gtgttgcaca cagttatctg tgagaaaccc
    26341 atggaagaga aatggaaata aaaagaactg acaccgcgat caaaaaacca aaaactgcaa
    26401 tacccacagc gtgatattga tcgcattaat ctttaaaacg gcgttgacag aagccataaa
    26461 ataagcaacc gaacgttcgt tcgagtatat tgggaacgat aaactctcat gacatatggc
    26521 tacggtgaat atttttgggg gggaattctc cgatcccggc tagattgtat tttgtacagg
    26581 gaaattaaag tatgaaacgc gggcacagtt atcaccagcc cgcgaaatga gaggatcagg
    26641 atgcggttaa gataccgcca atcatcgcgc gtaatccctg agcaaacgac gcttcacgcg
    26701 ccagatcttc cgcttcgata tcggccagta caccgtaggt cagatccagc agtaaacgtg
    26761 aagtcattga cgtgttgatg tctgggctta tctcgccttt ttgctgggca tcacgcatcc
    26821 gctgggcaac aaattcagtg atcgtcatat gcttattttt aagcatggcg gcgacctgtg
    26881 ggttacgcat cccttccgcc ataatttcga ccaccagcgc ccgttgccca aaggcttcat
    26941 gggtgagaga gacaatagac tcgaccatat agtcaacaaa atgaatccct tcaatcggtt
    27001 cccggaaacg ggccagcgcc ctctcctggt cctgtaagat aatcgcctga atcaaggctt
    27061 ctttggagat gaaatggtga tagagcgtcc cgggactaat ggcgcaggat ttacagatgg
    27121 ctttcatcga ggcactgtga aatccatttt ctgaaaaaat ctctctggca gcattgagga
    27181 tccgggtccg tgtcggttgt gcttcagtct gcatgttatc catcattaaa ccattatagt
    27241 tagcgtgaat aactagttta ccgcgttgtc aggtgagcgc ctatcattgc tgttgaatag
    27301 tgcgaaggca cactctattc gtatgctttt catccagcta ttggttaacc aatagccgtg
    27361 atttggctgc cagttgttta gcagagacgt tgatgaattg cacacgctat cccggtaaac
    27421 aacacatacg gctgtttacc ggttcgttag tgtattaatt gagctcctgt accccaccac
    27481 cggagacggt gagaatttgt ccgcttaccc agctggctgc aggcgagcaa aggaacagcg
    27541 ccgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc tgtaacattt
    27601 tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg
    27661 caatgccatt cacccgaata tttttttcac ccaggtcaaa tgccatattt ctgaccagat
    27721 gactggccgc agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg
    27781 ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa
    27841 gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatctgcca
    27901 ttggcatatc aaacggttta ggaccaccgc caccggcgtt gttaaccagg atatcaactt
    27961 tacccagctt actgacggca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat
    28021 cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca acatggttag
    28081 ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg
    28141 cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc
    28201 tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac
    28261 cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggacactacc aggtatagca
    28321 gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat
    28381 gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtggta ccaagttccc
    28441 gcgcaggcgt gctggcccag gtaacgggta tatcatcaag tgtggttggt gtcgcatcgg
    28501 taaatgccgc cagcgaaact tgttgctcaa agtaacgatc gactccgctt tccccgcttt
    28561 gccgccctgc tttgagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa
    28621 ccacagcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct
    28681 tctggctgga tgtgcactcc agcacccaat cgctgtgaaa cggcaggcca aattttagta
    28741 gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca
    28801 gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct
    28861 gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaata
    28921 tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt cctgcagccc
    28981 cggcaattac cacaccatcc acaccctgat tcagtaacag tgaaaaccgc tgtgccagct
    29041 gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt
    29101 ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac
    29161 caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca
    29221 gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac
    29281 taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg
    29341 ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac
    29401 gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgcctc
    29461 actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc
    29521 tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt
    29581 ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat
    29641 gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc cggtcctggg
    29701 caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag
    29761 tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaataaagg
    29821 cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg
    29881 gttgaccaat aaaggcattc tgccaaccac ggatgccgcg gttctgaaag ccaataacat
    29941 ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg tgatcgccgg
    30001 tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg atgcgctggc
    30061 attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg gccttgtcgg
    30121 cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct tgggccatat
    30181 gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt
    30241 gccgtttggt ctgcatcaca ttctggtagc attaattcgc tttaccgacg caggcggcac
    30301 gcaggaagtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag
    30361 ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggtaaaat
    30421 gcctgcgttt ctcggcggtc tgccgggtgc agcgttagct atgtatcact gcgcgcgccc
    30481 ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg
    30541 cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat
    30601 ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg
    30661 taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac
    30721 caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt
    30781 ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag
    30841 ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat
    30901 cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct
    30961 gcgtttgtct gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc
    31021 aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca
    31081 gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat
    31141 gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt
    31201 cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc
    31261 cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta
    31321 cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca
    31381 ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct atatggtttc
    31441 agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cgcccgccta
    31501 tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga
    31561 gaagcaggct gacgactggt tttgcgatat gggcaagctg gaagccgtgt tggcgaaacc
    31621 agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg
    31681 cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg ttatttccga
    31741 tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc
    31801 gcggggagac tgggcgttgc tgacgtcggg ctcgaaaagt ttcaatattc ccgcgctgac
    31861 cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa
    31921 aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta
    31981 tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta
    32041 tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac
    32101 ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc
    32161 gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg
    32221 tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg
    32281 attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg
    32341 cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatattcaga
    32401 aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg
    32461 atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc
    32521 ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc
    32581 tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct
    32641 gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg tcacggcctg cactatgtcg
    32701 agctgcgttt ttcaccaggc tacatggcaa tggcacatca gctgcctgta gcgggtgttg
    32761 tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc
    32821 ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct
    32881 ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc
    32941 cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg
    33001 tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg
    33061 cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg
    33121 ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag
    33181 cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta
    33241 acactgacga tcccagcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc
    33301 cagctgctgg gttatcccgc gagcaaatcc gccaggcaca gattaatggt ctggaaatgg
    33361 ctttcctcag cgctgaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa
    33421 atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg cgcgatgttt
    33481 ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac
    33541 cggattttca ccatcgccat ttaacgcatc acgaatagcc gcatagtaag ccggataatt
    33601 cccaggcacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac
    33661 gccatcacgc atatcgtagc cccagtcttc ctgcggtaga cgctcgccat ttttcagacg
    33721 ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata
    33781 ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata
    33841 ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc
    33901 taccgtcatg ctgaccggta aaccaaaaag cgtaatggcc tgatcaagaa gatgtggtgc
    33961 taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg
    34021 cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc
    34081 gagtaaacct tttagcgtca agaaatcgct atcccagcga cggttatgga atacagacag
    34141 cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggc
    34201 aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa
    34261 atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg
    34321 cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact
    34381 gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt
    34441 tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc
    34501 tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata
    34561 acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac
    34621 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
    34681 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
    34741 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
    34801 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
    34861 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
    34921 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
    34981 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
    35041 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc
    35101 cctttggtat taattatttt atggaccttt tatgactgtt caggactact tattaaaatt
    35161 tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct
    35221 gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct
    35281 ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt
    35341 ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat
    35401 ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg
    35461 gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg
    35521 cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac
    35581 aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt
    35641 attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact
    35701 atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg
    35761 tggaagatgg catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg
    35821 tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt
    35881 acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca
    35941 ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt
    36001 gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc
    36061 gcgatgggca tggggctggc aacaacgttt gtgatgacgc tggcgtctat ttgcgcctgg
    36121 cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt
    36181 attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg
    36241 gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc
    36301 ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt
    36361 ttttccgccg ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc
    36421 cttgctgtgg ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca
    36481 ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta
    36541 tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt
    36601 atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct
    36661 taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca
    36721 tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa
    36781 aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa
    36841 cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta
    36901 ttcaggcttg tccggtagac gccatcgttg gcgctacccg tgccatgcat acggtaatga
    36961 gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt
    37021 tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc
    37081 gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc
    37141 tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagtccaa cggtacaccc
    37201 ctgcgccagg taccactggc gcagcgtttt gttattccac tgaaacagca tattggcgct
    37261 gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt
    37321 ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg
    37381 ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat
    37441 ggtgaagact gctggatccc gcgcgacggc tgggccgatt atcgcactcg cagtcgcgaa
    37501 gagttaatcg agcgcataca tcagtttggt gtagccgggc tgggcggtgc aggattcccg
    37561 acaggcgtta aattgcaggg tggcggagat aagattgaaa cgttgattat caacgcggct
    37621 gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc
    37681 gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt
    37741 gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat
    37801 atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac
    37861 attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg
    37921 caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc
    37981 gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg
    38041 ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa
    38101 atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc
    38161 gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa
    38221 gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgcgga tcttttgccg
    38281 caacagttgt actggttcag caaaggtcag caacacgata aagctaccac gcataacatt
    38341 gctgattgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccctggtg
    38401 caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc
    38461 gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcggct
    38521 cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt
    38581 gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt
    38641 aaagcgggcg aacgcccgga taacagtgca attattgcag cacgggaagc ccgtaaagcg
    38701 caagccagag cgaaacaggc agaactgcag caaactaacg acgcagcaac cgttgctgat
    38761 ccacgtaaaa ctgccgttga agcagctatc gcccgcgcca aagcgcgcaa gctggaacag
    38821 caacaggcta a
//
LOCUS       DAZFVO020000025        37396 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFVO020000025 DAZFVO020000000
VERSION     DAZFVO020000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 37396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.025"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKNB_RS06165"
                     /old_locus_tag="pgaptmp_001233"
     CDS             complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKNB_RS06165"
                     /old_locus_tag="pgaptmp_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HGU4194788.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKNB_RS06170"
                     /old_locus_tag="pgaptmp_001234"
     CDS             complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKNB_RS06170"
                     /old_locus_tag="pgaptmp_001234"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGU4194789.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKNB_RS06175"
                     /old_locus_tag="pgaptmp_001235"
     CDS             complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKNB_RS06175"
                     /old_locus_tag="pgaptmp_001235"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGU4194790.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKNB_RS06180"
                     /old_locus_tag="pgaptmp_001236"
     CDS             complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKNB_RS06180"
                     /old_locus_tag="pgaptmp_001236"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HGU4194791.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5253..5372)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKNB_RS06185"
                     /old_locus_tag="pgaptmp_001237"
     CDS             complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKNB_RS06185"
                     /old_locus_tag="pgaptmp_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HGU4194792.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKNB_RS06190"
                     /old_locus_tag="pgaptmp_001238"
     CDS             complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKNB_RS06190"
                     /old_locus_tag="pgaptmp_001238"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4194793.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKNB_RS06195"
                     /old_locus_tag="pgaptmp_001239"
     CDS             complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKNB_RS06195"
                     /old_locus_tag="pgaptmp_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HGU4194794.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKNB_RS06200"
                     /old_locus_tag="pgaptmp_001240"
     CDS             6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKNB_RS06200"
                     /old_locus_tag="pgaptmp_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HGU4194795.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHKNB_RS06205"
                     /old_locus_tag="pgaptmp_001241"
     CDS             complement(7684..9036)
                     /gene="creD"
                     /locus_tag="ACHKNB_RS06205"
                     /old_locus_tag="pgaptmp_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HGU4194796.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDEPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQCQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTGAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHKNB_RS06210"
                     /old_locus_tag="pgaptmp_001242"
     CDS             complement(9094..10518)
                     /gene="creC"
                     /locus_tag="ACHKNB_RS06210"
                     /old_locus_tag="pgaptmp_001242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HGU4194797.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHKNB_RS06215"
                     /old_locus_tag="pgaptmp_001243"
     CDS             complement(10518..11207)
                     /gene="creB"
                     /locus_tag="ACHKNB_RS06215"
                     /old_locus_tag="pgaptmp_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HGU4194798.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHKNB_RS06220"
                     /old_locus_tag="pgaptmp_001244"
     CDS             complement(11220..11693)
                     /gene="creA"
                     /locus_tag="ACHKNB_RS06220"
                     /old_locus_tag="pgaptmp_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HGU4194799.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11904..12773
                     /gene="robA"
                     /locus_tag="ACHKNB_RS06225"
                     /old_locus_tag="pgaptmp_001245"
     CDS             11904..12773
                     /gene="robA"
                     /locus_tag="ACHKNB_RS06225"
                     /old_locus_tag="pgaptmp_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HGU4194800.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHKNB_RS06230"
                     /old_locus_tag="pgaptmp_001246"
     CDS             complement(12770..13417)
                     /gene="gpmB"
                     /locus_tag="ACHKNB_RS06230"
                     /old_locus_tag="pgaptmp_001246"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HGU4194801.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHKNB_RS06235"
                     /old_locus_tag="pgaptmp_001247"
     CDS             13469..13990
                     /gene="yjjX"
                     /locus_tag="ACHKNB_RS06235"
                     /old_locus_tag="pgaptmp_001247"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HGU4194802.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHKNB_RS06240"
                     /old_locus_tag="pgaptmp_001248"
     CDS             complement(14075..14401)
                     /gene="trpR"
                     /locus_tag="ACHKNB_RS06240"
                     /old_locus_tag="pgaptmp_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HGU4194803.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHKNB_RS06245"
                     /old_locus_tag="pgaptmp_001249"
     CDS             complement(14491..16428)
                     /gene="sltY"
                     /locus_tag="ACHKNB_RS06245"
                     /old_locus_tag="pgaptmp_001249"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HGU4194804.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16639..18306
                     /gene="ettA"
                     /locus_tag="ACHKNB_RS06250"
                     /old_locus_tag="pgaptmp_001250"
     CDS             16639..18306
                     /gene="ettA"
                     /locus_tag="ACHKNB_RS06250"
                     /old_locus_tag="pgaptmp_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HGU4194805.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHKNB_RS06255"
                     /old_locus_tag="pgaptmp_001251"
     CDS             complement(18613..19845)
                     /gene="nadR"
                     /locus_tag="ACHKNB_RS06255"
                     /old_locus_tag="pgaptmp_001251"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HGU4194806.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHKNB_RS06260"
                     /old_locus_tag="pgaptmp_001252"
     CDS             complement(19866..21248)
                     /gene="radA"
                     /locus_tag="ACHKNB_RS06260"
                     /old_locus_tag="pgaptmp_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGU4194807.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHKNB_RS06265"
                     /old_locus_tag="pgaptmp_001253"
     CDS             complement(21297..22265)
                     /gene="serB"
                     /locus_tag="ACHKNB_RS06265"
                     /old_locus_tag="pgaptmp_001253"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGU4194808.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHKNB_RS06270"
                     /old_locus_tag="pgaptmp_001254"
     CDS             22371..23015
                     /gene="ytjB"
                     /locus_tag="ACHKNB_RS06270"
                     /old_locus_tag="pgaptmp_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HGU4194809.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23043..24059
                     /gene="lplA"
                     /locus_tag="ACHKNB_RS06275"
                     /old_locus_tag="pgaptmp_001255"
     CDS             23043..24059
                     /gene="lplA"
                     /locus_tag="ACHKNB_RS06275"
                     /old_locus_tag="pgaptmp_001255"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HGU4194810.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            24091..24354
                     /locus_tag="ACHKNB_RS06280"
                     /old_locus_tag="pgaptmp_001256"
     CDS             24091..24354
                     /locus_tag="ACHKNB_RS06280"
                     /old_locus_tag="pgaptmp_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4194811.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHKNB_RS06285"
                     /old_locus_tag="pgaptmp_001257"
     CDS             complement(24515..25234)
                     /gene="deoD"
                     /locus_tag="ACHKNB_RS06285"
                     /old_locus_tag="pgaptmp_001257"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGU4194812.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHKNB_RS06290"
                     /old_locus_tag="pgaptmp_001258"
     CDS             complement(25314..26537)
                     /gene="deoB"
                     /locus_tag="ACHKNB_RS06290"
                     /old_locus_tag="pgaptmp_001258"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGU4194813.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHKNB_RS06295"
                     /old_locus_tag="pgaptmp_001259"
     CDS             complement(26589..27911)
                     /gene="deoA"
                     /locus_tag="ACHKNB_RS06295"
                     /old_locus_tag="pgaptmp_001259"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HGU4194814.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHKNB_RS06300"
                     /old_locus_tag="pgaptmp_001260"
     CDS             complement(28038..28817)
                     /gene="deoC"
                     /locus_tag="ACHKNB_RS06300"
                     /old_locus_tag="pgaptmp_001260"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGU4194815.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHKNB_RS06305"
                     /old_locus_tag="pgaptmp_001261"
     CDS             29075..30625
                     /gene="yjjI"
                     /locus_tag="ACHKNB_RS06305"
                     /old_locus_tag="pgaptmp_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HGU4194816.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHKNB_RS06310"
                     /old_locus_tag="pgaptmp_001262"
     CDS             30597..31460
                     /gene="yjjW"
                     /locus_tag="ACHKNB_RS06310"
                     /old_locus_tag="pgaptmp_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HGU4194817.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31775..32557)
                     /gene="yjjV"
                     /locus_tag="ACHKNB_RS06315"
                     /old_locus_tag="pgaptmp_001263"
     CDS             complement(31775..32557)
                     /gene="yjjV"
                     /locus_tag="ACHKNB_RS06315"
                     /old_locus_tag="pgaptmp_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4194818.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(32554..33627)
                     /gene="yjjU"
                     /locus_tag="ACHKNB_RS06320"
                     /old_locus_tag="pgaptmp_001264"
     CDS             complement(32554..33627)
                     /gene="yjjU"
                     /locus_tag="ACHKNB_RS06320"
                     /old_locus_tag="pgaptmp_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HGU4194819.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(33749..33910)
                     /gene="ytjA"
                     /locus_tag="ACHKNB_RS06325"
                     /old_locus_tag="pgaptmp_001265"
     CDS             complement(33749..33910)
                     /gene="ytjA"
                     /locus_tag="ACHKNB_RS06325"
                     /old_locus_tag="pgaptmp_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HGU4194820.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(34037..34642)
                     /gene="osmY"
                     /locus_tag="ACHKNB_RS06330"
                     /old_locus_tag="pgaptmp_001266"
     CDS             complement(34037..34642)
                     /gene="osmY"
                     /locus_tag="ACHKNB_RS06330"
                     /old_locus_tag="pgaptmp_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HGU4194821.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(35035..36621)
                     /gene="prfC"
                     /locus_tag="ACHKNB_RS06335"
                     /old_locus_tag="pgaptmp_001267"
     CDS             complement(35035..36621)
                     /gene="prfC"
                     /locus_tag="ACHKNB_RS06335"
                     /old_locus_tag="pgaptmp_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGU4194822.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            36840..37088
                     /locus_tag="ACHKNB_RS06340"
                     /old_locus_tag="pgaptmp_001268"
     CDS             36840..37088
                     /locus_tag="ACHKNB_RS06340"
                     /old_locus_tag="pgaptmp_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4194823.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
BASE COUNT         8909 a        10244 c         9373 g         8870 t
ORIGIN      
        1 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
       61 accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg gcggtccgtg
      121 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
      181 gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta attttactga
      241 cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa ccagggaaag
      301 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
      361 ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc aatcctaatc
      421 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt ctccataatt
      481 tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag
      541 attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
      601 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
      661 gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
      721 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
      781 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
      841 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg
      901 cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
      961 gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     1021 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     1081 gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgcg gcagctgcgc
     1141 aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc
     1201 tgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct tcaccagcgc
     1261 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaacgtaca
     1321 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     1381 attcggtaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     1441 cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc
     1501 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     1561 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     1621 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     1681 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     1741 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     1801 gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     1861 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     1921 gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg tcgggccgga
     1981 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     2041 gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc
     2101 aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc
     2161 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc ctggcttctg ccgtcgagac
     2221 tttaatcccc ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct
     2281 gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt
     2341 gtcgtaatga atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg
     2401 agtgtcatta agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga
     2461 acaggcgctg gagcctaagc ccgaaccgat tggcatattc ttttccagag tcatcgccac
     2521 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     2581 ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc
     2641 tgccgcctca accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc
     2701 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     2761 catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag
     2821 ctgtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     2881 ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     2941 ccacttcggc aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca
     3001 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg
     3061 acagattcgc cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag
     3121 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata
     3181 gcttacgcgc tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     3241 tttcccgcgc cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     3301 tataagaaag cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     3361 gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga
     3421 atttacgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     3481 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt
     3541 gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt
     3601 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     3661 cctgccagtt ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa
     3721 caccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     3781 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat
     3841 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     3901 ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg
     3961 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac
     4021 ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt
     4081 tcagttccaa gtagaactct tcctgcattg cccgttcagc ccgcacacag tcgctttgcg
     4141 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     4201 gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg
     4261 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     4321 cgtcttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     4381 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     4441 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     4501 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     4561 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     4621 caagtaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc aacaccatgt
     4681 gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     4741 attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg gtaacgttgt
     4801 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     4861 tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat
     4921 gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc gggaaccccg
     4981 gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat
     5041 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     5101 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     5161 tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     5221 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg
     5281 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
     5341 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
     5401 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
     5461 taatttactc acggcaggta accagttcag aagctgctat cagacactct ttttttaatc
     5521 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
     5581 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
     5641 gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc
     5701 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
     5761 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
     5821 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
     5881 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
     5941 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
     6001 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
     6061 gacatcgtgt aacgattcag ccagcgtcgg gaaaacttta atattatcaa taatatcacc
     6121 agatccatgt gcgacccagc gggtggctgg ctccaggtgc gcttgactat cgacaatccg
     6181 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
     6241 ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga ccaaataacc
     6301 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tattaacgta
     6361 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
     6421 aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
     6481 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
     6541 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
     6601 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggtc
     6661 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
     6721 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
     6781 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
     6841 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
     6901 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
     6961 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
     7021 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
     7081 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
     7141 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
     7201 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
     7261 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
     7321 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
     7381 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
     7441 aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccgcacg
     7501 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     7561 cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt gatctggaag
     7621 attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     7681 accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg ctttcatttt
     7741 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     7801 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     7861 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     7921 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     7981 tgccccggta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcactaa
     8041 tgaaagcccc accagtaaat attgcattgg gtgtaaacgt tgcgcggtga gcgtttcaaa
     8101 aacaaagaac gccataaaag tcagcgcaat cagcagaata gcgtacttag tcgcccggtc
     8161 agttaattgg cattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     8221 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa accagctgct
     8281 ttgccattgc gcctgaaaac ctgactcact aacttcccgt ttggctggca aaaaatcacc
     8341 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     8401 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     8461 cttgcgccag tccccttcag gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     8521 ggtgccgggt tcaatggtca gcgccgttcc gttaacttca ggcgctttca ccacaccaat
     8581 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggctc
     8641 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     8701 gacctgaccg gtataaatcc ctatcttgcg ttcttcaacg ttctgattgc catcaaccat
     8761 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcgtc
     8821 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     8881 cggcccgctg gtactttgcc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     8941 gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacaa
     9001 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     9061 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     9121 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     9181 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     9241 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     9301 atccagcacc ttaagggcga cgtgttcctg atccacttcg gcgcttagcg ttatgcgacc
     9361 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gctccagtaa
     9421 cgccggttca gcagcaacat taacctcagt gggcataaca tgtagagtga tgtttttttc
     9481 tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca catcaacaac
     9541 agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttctaccaa
     9601 tgcctgcata cgcgcatttt gcgtcagaat gttgtcggta aaacgagcca ccacttccgg
     9661 tggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     9721 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     9781 catactttcc agcgcctgcg caagcttacg caactcgcta ctgccgagtt caggaagagg
     9841 aacgggctta ttgtcagtga cggaatcggc atagcgagtg agccgggcaa tggagcggtt
     9901 gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccagcaaaa tggcgctggc
     9961 ccataaaatt cgccgctcgc tacgcttaat gaccggagcc atcgccgcgt tcggtttgcc
    10021 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
    10081 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
    10141 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
    10201 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
    10261 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
    10321 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
    10381 tgcggtatcg attaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
    10441 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
    10501 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
    10561 atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
    10621 tcaacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
    10681 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
    10741 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
    10801 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
    10861 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
    10921 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
    10981 aacgccggat ggagcgccag taactggcgg cataattcaa agccgctgat atcaggcaaa
    11041 ccaacatcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag caccggcaag
    11101 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac tagcgtgtcg
    11161 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
    11221 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
    11281 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata aaagcgcacg
    11341 acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc gccctgagct
    11401 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatagcc
    11461 gcatcggagg tatcttccgc cagacccaat ccccctttaa taccacctgt tttcgcccgg
    11521 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
    11581 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
    11641 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
    11701 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
    11761 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
    11821 cactgaatgc taaaacagca aaaaatgcta ttatccaatt atctgatgtc aggtgctcgt
    11881 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
    11941 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
    12001 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
    12061 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
    12121 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
    12181 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
    12241 gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa tttgtcaccc
    12301 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
    12361 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
    12421 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
    12481 aacaagaggt attctatacc actgcgttag cccaggatca ggcagatggc tatgtactga
    12541 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
    12601 gaaccggcgt gcaggagttt atcctgacag tatacggaac gtgcatgcca atgctcaatc
    12661 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
    12721 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
    12781 actcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca acaacccagc
    12841 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
    12901 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
    12961 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
    13021 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
    13081 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
    13141 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
    13201 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
    13261 tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
    13321 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
    13381 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
    13441 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
    13501 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
    13561 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
    13621 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
    13681 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
    13741 aaacaccagc cagcgcggcg aggcgcgttc ggctactttg ccgttacctg cggtgattct
    13801 gcagaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata ccggtattga
    13861 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gccgggaaac tcactcgcgc
    13921 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg ccgtgtatca
    13981 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
    14041 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
    14101 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
    14161 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
    14221 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    14281 gatttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    14341 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    14401 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    14461 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
    14521 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
    14581 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
    14641 acgcccggcg ctgttgccaa gccaggttcg cacccgtcca ggtccggcgt tataagctgc
    14701 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    14761 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctgctataac caggaataga
    14821 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
    14881 taccggtgat ttcactttcg gattccaggc gctctcctga cgggcaatcg ccatcgcata
    14941 gctttgcggg atctctttcc cactggtgta acgtttgaaa agatcgttgt aagccagcgg
    15001 gaatcgctct tccagatgat cccaaagctt cccggcgatc gttgcctgaa cgctaagatc
    15061 ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt ttgacttgct
    15121 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    15181 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    15241 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
    15301 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
    15361 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
    15421 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
    15481 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
    15541 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
    15601 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
    15661 atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa aatcggtcgc
    15721 tccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    15781 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
    15841 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    15901 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    15961 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    16021 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    16081 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    16141 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    16201 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
    16261 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    16321 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    16381 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
    16441 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    16501 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    16561 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    16621 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    16681 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    16741 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    16801 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    16861 ccgcaggaac cgcaactgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    16921 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    16981 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    17041 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct gcgtctgcca
    17101 gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    17161 cgcctgcttc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    17221 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgttgtggcg
    17281 attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    17341 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    17401 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    17461 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    17521 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    17581 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggt
    17641 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcaat cgtcgggatc
    17701 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctctgg tcaggaacag
    17761 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcatcggt tgatcagttc
    17821 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    17881 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    17941 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    18001 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    18061 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    18121 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat cctggactac
    18181 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga agagtacaag
    18241 aaacgtacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    18301 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    18361 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    18421 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    18481 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    18541 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    18601 gtttcatcgc ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag
    18661 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    18721 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    18781 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    18841 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    18901 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    18961 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    19021 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    19081 gaagatattg gcaagtttgt ttaccaaagt ggatttaccg ctcgactcgc caccaaggat
    19141 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    19201 cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    19261 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    19321 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    19381 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    19441 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    19501 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    19561 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    19621 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    19681 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    19741 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    19801 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    19861 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    19921 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    19981 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    20041 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    20101 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    20161 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    20221 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    20281 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    20341 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    20401 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    20461 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    20521 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    20581 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    20641 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    20701 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    20761 cagatctgtt cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg
    20821 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cagtgacata cagcgttttc
    20881 atctgctggg ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcacctggg
    20941 ttaccgccaa tcagaatggc gcttcctggc accacgccgc cgcctagtac gcggtcgaac
    21001 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    21061 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    21121 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    21181 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    21241 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    21301 ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    21361 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    21421 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    21481 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    21541 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    21601 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    21661 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    21721 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    21781 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    21841 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    21901 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    21961 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    22021 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    22081 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    22141 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    22201 gaaagaggca gaccaggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    22261 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    22321 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    22381 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    22441 tggcgctgat gcagggagcg tcatggttta gtcaaaacca ccagcgacaa cgtaatccac
    22501 agctggaaga actggctcgc accctggcgc gtcaggtaac gctgaacgtt gcaccgctga
    22561 tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    22621 aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcacgttctg
    22681 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagca ggcggctatt
    22741 ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc tatctgcgcc
    22801 tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    22861 ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc
    22921 tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    22981 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    23041 ttatgtccac attacgcctg ctcatttctg actcttacga tccgtggttt aacctggcgg
    23101 tggaggaatg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    23161 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    23221 ggatggaaga agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc
    23281 acgatctcgg caatacctgt tttaccttta tggctggcaa gccggagtac gataaaacca
    23341 tctctacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    23401 gacgtaacga tctggtggtg aaaaccgccg aaggcgaccg caaagtctca ggctcggcct
    23461 atcgcgaaac caaagatcgt ggcttccacc acggcacctt gctgctcaat gccgacctta
    23521 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    23581 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    23641 tttgcgaggc cataaccgag gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa
    23701 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgccaga
    23761 gtagctggga atggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct
    23821 ttacctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    23881 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccgga cgactgcaag
    23941 gctgcctgta ccgcgcagat atgctgcaac aggagtgcga agcgctgttg gttgacttcc
    24001 cggaacagga aaaagagcta cgggagttat cgacgtggat agcgggggcg gtgcggtaac
    24061 tcgctgatta aaatatccag ggaaggaaat atgatcgatc tggaaaacca ggaacgggag
    24121 attatcaatt taatgttcag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    24181 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaacag
    24241 atcgaaaaga cggaacggct tagctcaaac atcaaaagca aaatggcaga aatttatggt
    24301 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    24361 tcgtgaatag atacttgaat gtattttatt ttctatccat atgaaaaata atagtaaatc
    24421 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtcc cccactccgg
    24481 cttaacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    24541 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    24601 gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt cagggctttc gcgccaaatt
    24661 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    24721 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    24781 caacgcgagc atcaacgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    24841 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    24901 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    24961 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    25021 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttgtaag
    25081 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    25141 tttcagcaat atacttcgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    25201 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatca acatgttttc
    25261 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    25321 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctgaccaata
    25381 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    25441 accggaatgt gttcacgcgt gtggtcagta ccggtccagg tcggatcaca accgtggtca
    25501 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    25561 tcgaacagct ccagacccgc agcataaccg gcgacgtcgc gacggtggcc ccaggaagag
    25621 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    25681 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    25741 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    25801 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    25861 cgctggaagt taccggcttt gtcgccaata aacggacgag cgataacacg accgatgttg
    25921 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccaag
    25981 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    26041 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    26101 tggcagttac cgagataacc aggcaggtta gcgcgttcaa ccagtttatc cagcagctct
    26161 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    26221 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    26281 tacgcgccga taacttcagc gttgccgtcc attcctgccg gaatgaaacc ggtagaacct
    26341 tcatgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    26401 ccgttatcag cttcgccttt ggcacaggct tctgcgatat gacccagggt gtcagccccg
    26461 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    26521 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    26581 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    26641 gcaagtttaa ttgccgcttt caccgctttc gccgcgtcct gccagctgtt ttcgtctttc
    26701 gcgtggataa cagccagcgg acgctgtccg tctacctggt cgcccagacg cgccatatca
    26761 gtaaagccga cgctgtaatc gatggtgtca gatgcctgac ggcgtccgcc gcccattgca
    26821 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    26881 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    26941 gtcgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    27001 agcaccgcct gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagatc
    27061 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattcaccc
    27121 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    27181 tgattcatgt cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    27241 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    27301 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    27361 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    27421 tccgccggag ccagtgagct ggtctggccg ataatcgcta cgccgacgtc tttaataatt
    27481 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    27541 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    27601 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtggagtgt
    27661 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    27721 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    27781 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag
    27841 aaacgaattt cttcatcgct tagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    27901 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    27961 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatta
    28021 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    28081 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    28141 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    28201 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    28261 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    28321 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    28381 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    28441 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    28501 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    28561 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    28621 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    28681 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    28741 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    28801 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    28861 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    28921 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    28981 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    29041 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    29101 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    29161 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    29221 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    29281 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    29341 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    29401 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    29461 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    29521 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    29581 tacccgtgca atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    29641 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    29701 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    29761 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    29821 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    29881 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    29941 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    30001 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    30061 gtgtgagaaa gaagggattg ccgcacgtta cggtaaagaa gccgccgcaa atgaagtggg
    30121 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggatg
    30181 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatattg gcaccacgcc
    30241 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    30301 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    30361 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    30421 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtacg
    30481 tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    30541 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    30601 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    30661 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    30721 ggacgatggg acgttgcaat gactgtggag agtgcgtgcc acagtgtccg catcaggcgt
    30781 tgcagattgt tgacggcaaa gtgctgtgga acgctgtggt ctgcgagcag tgtgatacct
    30841 gcctgaagat gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    30901 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    30961 aagccacgac ccaactgccg tttgtagtgg cgctgtttac tgctatcaaa aacgatccgc
    31021 aactgcgcca cctcacctgc ctggtggaca gtaacggcat gttgagcgaa accggctggg
    31081 aaaaattact cccggtgtgt gacggcgcaa tgctcgatct caaagcgtgg gggagcgaat
    31141 gtcatcaaca cttaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgcttg
    31201 cagagcgcgg caagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    31261 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    31321 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    31381 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    31441 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca gacttgtagg
    31501 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgcggcgt
    31561 gaacgcctta tccggcctac ggttcggcac aaacctgtag gcatgataag acgcgtcaag
    31621 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    31681 cggttcggca caaacctgta ggcatgataa gacgcgttaa gcgtcgcatc aggcattatg
    31741 cgccgactgc cggatgctgc ctacgaaggg taaactaccc actaacgcta aacaacgcat
    31801 acgtattatt aagcaacact tcggcaatct catcttctgg ttccgggcgc aactcgcaaa
    31861 gcacgtcaaa cacccgcaca gcctgttccg gtcggttagg ctgcccctga aagccgttga
    31921 gcggcatatc cggcgcgtcg gtttccagca acaacgatgc cagcggtaat tttgcgatga
    31981 catcgcgggt tttactggcg cgtggatagg tgatagtacc gcctacgccg attttgtagc
    32041 ccagctgtac aaaccgctcg gcctgttgca ggctgccgga aaaaccgtga accacgccag
    32101 tgcacggtaa atcgtgacgt ttaagatgca tcgccagttt gtcgtgtgta cgccgggaat
    32161 gaaggatcac cggcagatcg tagcgtttcg ccagtttcag ctgttcgtcg agtaaccact
    32221 gctgcctctc aaattgcgga tcgtcgccga agagatccag accgatctcc cccaccgcca
    32281 ccaccttcgc cggacgcctt tccagcgcct gctgtagctg atcaagagac acatcgctat
    32341 gtttttccaa catccccgga tgcaacccca atgcggcata cagcggttga tatttttccg
    32401 ctaacgcctg cacgcgggca aaattttccg cctctgtcgc cggaacaatg atcttgccta
    32461 cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc gccactaaac ggcgggaaat
    32521 caaaatggca gtgggtatcg ataaaacggc aaatcaagcc agatcctcat tgttaaatgt
    32581 cgggtcgttc gcctgcggag catcgctcac ttcggcaacc agcgtatcgt tggcgactgg
    32641 cgcaggcgga ataacgatcg attccggcgt caccactggc accagatggc gggtaagcgg
    32701 cgctttttca gttaatagct tgccaaccgt ggcgaggaaa taacgaccgc ataaacgccc
    32761 aagtttatag tcttcacgca gcgccggaat ccgactgcca agcgcgatac tatgtaatgg
    32821 tttcggtgga taaatttcga atatccgcag cttgcccggt ggtttctcaa taaattgctg
    32881 aatgtcacga tagctggttt catgatgctg caccagattg accagcggct gcaggctact
    32941 gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc gtgtagtaca tttgtgacgg
    33001 cacagtgcgg atgacgacca acgttttagc gccctgcctt gccgcctctt taaccggaat
    33061 cgcatcactg atcccgccat ccaagtagtt aatgccttcc agcgacactc cgctacgata
    33121 aaagccaggt atcgcactgg aggcgcgaat cacatccagc cagttttgtt tggttggtaa
    33181 aaagtaatta ggcgcgtagt catcctgacg acaggcgcac atataaaacg atttgccgct
    33241 gtcaaacaac cgcgcggcgg tatccatttg caacggcatc tggcttgcgg tggcctccac
    33301 cagccagtcg agatcgataa gatttcctcc acggacaaag cgcaatggat cgaaaaattc
    33361 gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa ccgggctgat tgcagataaa
    33421 cgccgagagg ttctgcgccc cggcagatgt gccgagataa agatcgaaag gattaaactg
    33481 cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt ccacgctgtc cgccgccttc
    33541 gcacaccaga gctattcgtc caggctggaa cggccttagc gacaacggcg caatattacc
    33601 aagcgttaca ggtattcgct gccccacctc tgccttcctg tttttgttgt gaaagaatga
    33661 caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc cagtccgctg gactggcttt
    33721 ggataaaatt ttcttaatgg tctgaaatct agggtcgttt tcggcccatg aacaaactca
    33781 ccaggaacag aataatcccg acgacaaaga caattttagc tgcgcctgca gcggtaccgg
    33841 ccagaccacc aaacccaagt gcggcggcga ttaacgcgat aaccagaaat atgatgcccc
    33901 aacgaaacat aagcgtctcc tttaccatag ttaatgtcac cgcttgatat gagcgtgaaa
    33961 atcactcaat gcgatatttt tagtgtggtg cacatcgcgc ctcccgacaa agttcgggag
    34021 gacgaattac gacgaattac ttagttttca gatcattttt aacgcttttc acaccatcta
    34081 ccgctttggc gatactttca gcacggtcgc tctgtgcctg ggaatcgacg gtaccggaga
    34141 gctgaaccac gccgtcggtg gtttcaactt tcacatgacg ggaagggacg atatcgtccg
    34201 ccagcagttt ggctttgatt tcactggtgg tggcggtgtc acccgcgtag cccttcacag
    34261 agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac agaggtcacc ccttcaacgc
    34321 ctttcgccac tttcactgcc tcttcggcct gggcctggct ttcaacgaaa ccgctcaggg
    34381 tcacgacttt ttgatcggtt tttacagaga tatcggtgct cttgatgttg tcatgatcca
    34441 ccagggccgc cttcactttc gcggtgatgg cgctgtcatc catgaaatta ccgactttat
    34501 tcatagagct atcgactttt tgccctgcgc tttcattggt agtctgtgcg ttgttttccg
    34561 cgtaggcaga gccggtcgcg acggcagagg tcaacattac agccagcaga gttttcgaaa
    34621 tcttcagtct tgtcatagtc atcgatttat tcctgtatgt ttgctcgtaa tttgagcctg
    34681 gcaacacgag gttgcattgc tgaataggga gagacttcac cctctacaga agtcaatggt
    34741 cgccatcaca aaagcgatgg gtgatgaata acgaccatta cagcctctga atcagttatt
    34801 aatatcggta gaatgacaat cgacggcttt agatactgat atctacgcat tgaacggtat
    34861 ttaatgccgt cagaaatgtc attactttgt taaatataga tcacaatttt gaaaccgctc
    34921 gggatatcag cgaaaacata agcaaaagtg aatgttttaa gaacattccg taagcggcta
    34981 ataaggaagg gaaattgaca gggcgaggcg gttgccgcgc cctggaggca agaattaatg
    35041 ctcgcgggtc tggtggaact gaacgtccgg ataacgttcc tgtgccaggc gcaggttaac
    35101 catgctggta gcgatgtaag cgagattatc gccgccatca agcgccagtt ggctttcgtt
    35161 cttacgcttg aactcttcaa atttcttcgc gtccgcacat tctacccacc gggcggtggc
    35221 aacgttgact gattcatata ctgcttcaac gttgtattcg ctcttcaggc gcgataccac
    35281 tacatcaaac tgcagcacac caaccgcacc aacgatcagg tcgttgttgg agatcggacg
    35341 gaacacctgc accgcgccct cttcggaaag ctgtaccagc cctttgagca gctgtttttg
    35401 cttcagcgga tctttcaggc ggatacgacg gaacagttct ggtgcgaagt tcggaatacc
    35461 ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg atctgaatgg tgccgtggtt
    35521 gtgcaggccg aggatatcgc caggatacgc ttcttcaacg tgcgaacggt cacccgccat
    35581 aaaggtcagc gcgtcagaga tcaccacgtc tttcgcggtg cgcacctggc gcagcttcat
    35641 gcctttttca tatttaccgg ataccacgcg cataaacgcc acgcggtcgc ggtgtttcgg
    35701 gtccatgttg gcctgaattt taaatacgaa gccggtaaat ttgtcttcgc tcgcttgtac
    35761 ggtacgggta tcagtctgac gcggcatcgg cgcaggtgcc cactccacca ggccatccaa
    35821 catatgatca acgccgaagt tacccagcgc agtaccgaag aataccggag tgatttcgcc
    35881 cgcaaggaac agctctttgt cgaactcgtt agacgcgcct ttaaccagtt ccagttcgtc
    35941 acgcagctgc tgtgccagat cttcaccaac cgcagcatcg agatccgggt tattcagccc
    36001 tttaacaatg cggacttcct gaatggtgtg ccctttaccg ctctgataga gataggtttc
    36061 atctttataa aggtggtaaa cgcctttaaa cagcttgccg cagccaattg gccaggtgat
    36121 cggtgcgcag ccaattttca gctcgttctc aacttcatcg agcaattcca tcgggtcgcg
    36181 gatatcacgg tcaagtttgt tcataaaggt gaggatcggc gtgtcgcgca gacgggtaac
    36241 ttccatcagc ttacgggtac gatcttcaac accttttgcg gcgtcgataa ccatcaggca
    36301 gcagtccacc gccgtcaggg tacgataggt atcttccgag aagtcttcat gccccggggt
    36361 gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc atcacagacg tagtaatgga
    36421 gatcccacgc tgcttttcca tctccatcca gtccgactta gcgtgctggt tggaaccacg
    36481 gccttttact gtaccggcgg tctgaatggc ctgtccgaac agcagcacct tctcggtgat
    36541 ggtagtctta ccggcgtccg ggtgagaaat aattgcaaag gttcttcttt tcgctacctc
    36601 tgcggccaaa aggcttgtca taattgttgt ctctttgatt atgtacaatc atatgtacaa
    36661 agaaaaattc atgccgctga ttgtacattt tggaggtatc agagcatagc agaacatgat
    36721 cagtaggtgt atttgtagcc aaagaacatg tgtatatgca gcaatctttg ctctattgac
    36781 actgtttatt catacagtta aaaataacac tgtatacaaa cacagtgaca ggaggtgtta
    36841 tgcgtattga gatctgtatc gctaaagaaa aaatctgcaa aatgccaaaa ggctctgttc
    36901 ctgccctgca acaggaaatg ctacgacgtg tcggtaagcg ttatgatgat gtagatgtga
    36961 ttgtgaaatc tgccagtaat gatggactca caattttaag agccgtggat aaagaatccg
    37021 cgcaagaatt tgtacaggaa acgctgcagg aagtctggga aacagcggat gactggttta
    37081 tccggtgaca tcagcacggg gagctggcag ctcccctctt ttattgtgtt atcagtgcag
    37141 caagtacaca gagtgttacc acaccaccca gccatacaaa cagcatgaag cacatcgcac
    37201 ccgctgtgta ttttcgcttt ccgcatctga ttttttcctg ctccattctc actgccaccg
    37261 ttcaactctc attacccgac ttcacgaata tgcattccgg ctacgcacgt ccaaccaggt
    37321 gcttcgcact cctgtactta agaccgtttt taccaagtcc catatacaga aaggtgttca
    37381 ccgtcttcgt gtttat
//
LOCUS       DAZFVO020000026        35631 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFVO020000026 DAZFVO020000000
VERSION     DAZFVO020000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.026"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..591
                     /locus_tag="ACHKNB_RS06345"
                     /old_locus_tag="pgaptmp_001269"
     CDS             <1..591
                     /locus_tag="ACHKNB_RS06345"
                     /old_locus_tag="pgaptmp_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HGU4194824.1"
                     /translation="LSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFLESVIT
                     TAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            644..1615
                     /locus_tag="ACHKNB_RS06350"
                     /old_locus_tag="pgaptmp_001270"
     CDS             644..1615
                     /locus_tag="ACHKNB_RS06350"
                     /old_locus_tag="pgaptmp_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HGU4194825.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQEVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(1729..2178)
                     /gene="rplI"
                     /locus_tag="ACHKNB_RS06355"
                     /old_locus_tag="pgaptmp_001271"
     CDS             complement(1729..2178)
                     /gene="rplI"
                     /locus_tag="ACHKNB_RS06355"
                     /old_locus_tag="pgaptmp_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGU4194826.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2220..2447)
                     /gene="rpsR"
                     /locus_tag="ACHKNB_RS06360"
                     /old_locus_tag="pgaptmp_001272"
     CDS             complement(2220..2447)
                     /gene="rpsR"
                     /locus_tag="ACHKNB_RS06360"
                     /old_locus_tag="pgaptmp_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGU4194827.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(2452..2766)
                     /gene="priB"
                     /locus_tag="ACHKNB_RS06365"
                     /old_locus_tag="pgaptmp_001273"
     CDS             complement(2452..2766)
                     /gene="priB"
                     /locus_tag="ACHKNB_RS06365"
                     /old_locus_tag="pgaptmp_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HGU4194828.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(2773..3168)
                     /gene="rpsF"
                     /locus_tag="ACHKNB_RS06370"
                     /old_locus_tag="pgaptmp_001274"
     CDS             complement(2773..3168)
                     /gene="rpsF"
                     /locus_tag="ACHKNB_RS06370"
                     /old_locus_tag="pgaptmp_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGU4194829.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            3495..3770
                     /gene="yjfY"
                     /locus_tag="ACHKNB_RS06375"
                     /old_locus_tag="pgaptmp_001275"
     CDS             3495..3770
                     /gene="yjfY"
                     /locus_tag="ACHKNB_RS06375"
                     /old_locus_tag="pgaptmp_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HGU4194830.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3899..4585)
                     /gene="ulaF"
                     /locus_tag="ACHKNB_RS06380"
                     /old_locus_tag="pgaptmp_001276"
     CDS             complement(3899..4585)
                     /gene="ulaF"
                     /locus_tag="ACHKNB_RS06380"
                     /old_locus_tag="pgaptmp_001276"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HGU4194831.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIASFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4585..5439)
                     /gene="ulaE"
                     /locus_tag="ACHKNB_RS06385"
                     /old_locus_tag="pgaptmp_001277"
     CDS             complement(4585..5439)
                     /gene="ulaE"
                     /locus_tag="ACHKNB_RS06385"
                     /old_locus_tag="pgaptmp_001277"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HGU4194832.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5449..6099)
                     /gene="ulaD"
                     /locus_tag="ACHKNB_RS06390"
                     /old_locus_tag="pgaptmp_001278"
     CDS             complement(5449..6099)
                     /gene="ulaD"
                     /locus_tag="ACHKNB_RS06390"
                     /old_locus_tag="pgaptmp_001278"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4194833.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(6113..6577)
                     /gene="ulaC"
                     /locus_tag="ACHKNB_RS06395"
                     /old_locus_tag="pgaptmp_001279"
     CDS             complement(6113..6577)
                     /gene="ulaC"
                     /locus_tag="ACHKNB_RS06395"
                     /old_locus_tag="pgaptmp_001279"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HGU4194834.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6587..6892)
                     /gene="ulaB"
                     /locus_tag="ACHKNB_RS06400"
                     /old_locus_tag="pgaptmp_001280"
     CDS             complement(6587..6892)
                     /gene="ulaB"
                     /locus_tag="ACHKNB_RS06400"
                     /old_locus_tag="pgaptmp_001280"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HGU4194835.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEIIVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6908..8305)
                     /gene="ulaA"
                     /locus_tag="ACHKNB_RS06405"
                     /old_locus_tag="pgaptmp_001281"
     CDS             complement(6908..8305)
                     /gene="ulaA"
                     /locus_tag="ACHKNB_RS06405"
                     /old_locus_tag="pgaptmp_001281"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4194836.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8660..9724
                     /gene="ulaG"
                     /locus_tag="ACHKNB_RS06410"
                     /old_locus_tag="pgaptmp_001282"
     CDS             8660..9724
                     /gene="ulaG"
                     /locus_tag="ACHKNB_RS06410"
                     /old_locus_tag="pgaptmp_001282"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGU4194837.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9832..10587
                     /gene="ulaR"
                     /locus_tag="ACHKNB_RS06415"
                     /old_locus_tag="pgaptmp_001283"
     CDS             9832..10587
                     /gene="ulaR"
                     /locus_tag="ACHKNB_RS06415"
                     /old_locus_tag="pgaptmp_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HGU4194838.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10584..11333)
                     /gene="yjfP"
                     /locus_tag="ACHKNB_RS06420"
                     /old_locus_tag="pgaptmp_001284"
     CDS             complement(10584..11333)
                     /gene="yjfP"
                     /locus_tag="ACHKNB_RS06420"
                     /old_locus_tag="pgaptmp_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4194839.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPTDESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11515..11844
                     /gene="bsmA"
                     /locus_tag="ACHKNB_RS06425"
                     /old_locus_tag="pgaptmp_001285"
     CDS             11515..11844
                     /gene="bsmA"
                     /locus_tag="ACHKNB_RS06425"
                     /old_locus_tag="pgaptmp_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HGU4194840.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11993..12268
                     /gene="yjfN"
                     /locus_tag="ACHKNB_RS06430"
                     /old_locus_tag="pgaptmp_001286"
     CDS             11993..12268
                     /gene="yjfN"
                     /locus_tag="ACHKNB_RS06430"
                     /old_locus_tag="pgaptmp_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HGU4194841.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12385..14010)
                     /gene="aidB"
                     /locus_tag="ACHKNB_RS06435"
                     /old_locus_tag="pgaptmp_001287"
     CDS             complement(12385..14010)
                     /gene="aidB"
                     /locus_tag="ACHKNB_RS06435"
                     /old_locus_tag="pgaptmp_001287"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HGU4194842.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(14094..15246)
                     /gene="yjfC"
                     /locus_tag="ACHKNB_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /pseudo
     CDS             complement(14094..15246)
                     /gene="yjfC"
                     /locus_tag="ACHKNB_RS06440"
                     /old_locus_tag="pgaptmp_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
     gene            complement(15249..15887)
                     /gene="yjfM"
                     /locus_tag="ACHKNB_RS06445"
                     /old_locus_tag="pgaptmp_001289"
     CDS             complement(15249..15887)
                     /gene="yjfM"
                     /locus_tag="ACHKNB_RS06445"
                     /old_locus_tag="pgaptmp_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HGU4194843.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15897..16295)
                     /gene="yjfL"
                     /locus_tag="ACHKNB_RS06450"
                     /old_locus_tag="pgaptmp_001290"
     CDS             complement(15897..16295)
                     /gene="yjfL"
                     /locus_tag="ACHKNB_RS06450"
                     /old_locus_tag="pgaptmp_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HGU4194844.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16313..16972)
                     /gene="yjfK"
                     /locus_tag="ACHKNB_RS06455"
                     /old_locus_tag="pgaptmp_001291"
     CDS             complement(16313..16972)
                     /gene="yjfK"
                     /locus_tag="ACHKNB_RS06455"
                     /old_locus_tag="pgaptmp_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HGU4194845.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(17023..17721)
                     /gene="yjfJ"
                     /locus_tag="ACHKNB_RS06460"
                     /old_locus_tag="pgaptmp_001292"
     CDS             complement(17023..17721)
                     /gene="yjfJ"
                     /locus_tag="ACHKNB_RS06460"
                     /old_locus_tag="pgaptmp_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HGU4194846.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17740..18141)
                     /gene="yjfI"
                     /locus_tag="ACHKNB_RS06465"
                     /old_locus_tag="pgaptmp_001293"
     CDS             complement(17740..18141)
                     /gene="yjfI"
                     /locus_tag="ACHKNB_RS06465"
                     /old_locus_tag="pgaptmp_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HGU4194847.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(18268..18999)
                     /gene="rlmB"
                     /locus_tag="ACHKNB_RS06470"
                     /old_locus_tag="pgaptmp_001294"
     CDS             complement(18268..18999)
                     /gene="rlmB"
                     /locus_tag="ACHKNB_RS06470"
                     /old_locus_tag="pgaptmp_001294"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGU4194848.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(19179..21620)
                     /gene="rnr"
                     /locus_tag="ACHKNB_RS06475"
                     /old_locus_tag="pgaptmp_001295"
     CDS             complement(19179..21620)
                     /gene="rnr"
                     /locus_tag="ACHKNB_RS06475"
                     /old_locus_tag="pgaptmp_001295"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGU4194849.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(21659..22084)
                     /gene="nsrR"
                     /locus_tag="ACHKNB_RS06480"
                     /old_locus_tag="pgaptmp_001296"
     CDS             complement(21659..22084)
                     /gene="nsrR"
                     /locus_tag="ACHKNB_RS06480"
                     /old_locus_tag="pgaptmp_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HGU4194850.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(22289..23587)
                     /gene="purA"
                     /locus_tag="ACHKNB_RS06485"
                     /old_locus_tag="pgaptmp_001297"
     CDS             complement(22289..23587)
                     /gene="purA"
                     /locus_tag="ACHKNB_RS06485"
                     /old_locus_tag="pgaptmp_001297"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGU4194851.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(23691..23888)
                     /gene="yjeT"
                     /locus_tag="ACHKNB_RS06490"
                     /old_locus_tag="pgaptmp_001298"
     CDS             complement(23691..23888)
                     /gene="yjeT"
                     /locus_tag="ACHKNB_RS06490"
                     /old_locus_tag="pgaptmp_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HGU4194852.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(23970..24974)
                     /gene="hflC"
                     /locus_tag="ACHKNB_RS06495"
                     /old_locus_tag="pgaptmp_001299"
     CDS             complement(23970..24974)
                     /gene="hflC"
                     /locus_tag="ACHKNB_RS06495"
                     /old_locus_tag="pgaptmp_001299"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HGU4194853.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(24977..26236)
                     /gene="hflK"
                     /locus_tag="ACHKNB_RS06500"
                     /old_locus_tag="pgaptmp_001300"
     CDS             complement(24977..26236)
                     /gene="hflK"
                     /locus_tag="ACHKNB_RS06500"
                     /old_locus_tag="pgaptmp_001300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HGU4194854.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGNIMDQRRANAQRNDYQRQGE"
     gene            complement(26322..27602)
                     /gene="hflX"
                     /locus_tag="ACHKNB_RS06505"
                     /old_locus_tag="pgaptmp_001301"
     CDS             complement(26322..27602)
                     /gene="hflX"
                     /locus_tag="ACHKNB_RS06505"
                     /old_locus_tag="pgaptmp_001301"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HGU4194855.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(27678..27986)
                     /gene="hfq"
                     /locus_tag="ACHKNB_RS06510"
                     /old_locus_tag="pgaptmp_001302"
     CDS             complement(27678..27986)
                     /gene="hfq"
                     /locus_tag="ACHKNB_RS06510"
                     /old_locus_tag="pgaptmp_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HGU4194856.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(28072..29022)
                     /gene="miaA"
                     /locus_tag="ACHKNB_RS06515"
                     /old_locus_tag="pgaptmp_001303"
     CDS             complement(28072..29022)
                     /gene="miaA"
                     /locus_tag="ACHKNB_RS06515"
                     /old_locus_tag="pgaptmp_001303"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGU4194857.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(29015..30862)
                     /gene="mutL"
                     /locus_tag="ACHKNB_RS06520"
                     /old_locus_tag="pgaptmp_001304"
     CDS             complement(29015..30862)
                     /gene="mutL"
                     /locus_tag="ACHKNB_RS06520"
                     /old_locus_tag="pgaptmp_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGU4194858.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(30872..32209)
                     /gene="amiB"
                     /locus_tag="ACHKNB_RS06525"
                     /old_locus_tag="pgaptmp_001305"
     CDS             complement(30872..32209)
                     /gene="amiB"
                     /locus_tag="ACHKNB_RS06525"
                     /old_locus_tag="pgaptmp_001305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HGU4194859.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(32228..32689)
                     /gene="tsaE"
                     /locus_tag="ACHKNB_RS06530"
                     /old_locus_tag="pgaptmp_001306"
     CDS             complement(32228..32689)
                     /gene="tsaE"
                     /locus_tag="ACHKNB_RS06530"
                     /old_locus_tag="pgaptmp_001306"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGU4194860.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(32661..34208)
                     /gene="nnr"
                     /locus_tag="ACHKNB_RS06535"
                     /old_locus_tag="pgaptmp_001307"
     CDS             complement(32661..34208)
                     /gene="nnr"
                     /locus_tag="ACHKNB_RS06535"
                     /old_locus_tag="pgaptmp_001307"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HGU4194861.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            34207..35346
                     /gene="queG"
                     /locus_tag="ACHKNB_RS06540"
                     /old_locus_tag="pgaptmp_001308"
     CDS             34207..35346
                     /gene="queG"
                     /locus_tag="ACHKNB_RS06540"
                     /old_locus_tag="pgaptmp_001308"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HGU4194862.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLIGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(35329..35382)
                     /locus_tag="ACHKNB_RS06545"
                     /old_locus_tag="pgaptmp_001309"
     CDS             complement(35329..35382)
                     /locus_tag="ACHKNB_RS06545"
                     /old_locus_tag="pgaptmp_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194863.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         8585 a         9746 c         8734 g         8566 t
ORIGIN      
        1 ctaagcggta tttttactcc aaccgaagcc tccgcaattg ctgtaattta tacccttttc
       61 cttgcactgg ttctttatcg tgaaatctca gttaaagatt tacctaaaat atttctggaa
      121 tccgttatta ccactgcaat tgtcttgtta ttaattggtt cttcgatggg aatgtcatgg
      181 gcaatgtcaa acgctgacgt tcccttcctg attctcgatt tgcttaacac tatttctgac
      241 aacccaataa taatcctgct tattatcaat attatcctgt tgattatcgg cacttttatg
      301 gatatgacac cggcggtact gatttttacg ccgatatttt taccggttgt caccgaactg
      361 ggcatggacc cgattcactt tggtattgtc atggtgctga acatgtgtat cggtatttgt
      421 actccgcctg taggcagtgt gttatttgtc ggttgtagtg tatctaaatt accgattaat
      481 aaaattatca aacctatgct gccattctat gccgtgatgg tattggtgct ggcaatggtg
      541 acctatattc cgcaaatcag tatggcattg ccaagagcac tgggttactg atgcgcattc
      601 actagcggca cctgccgcca tatttattag aaaaggaaca tctatgaaaa taaaagtatc
      661 cgctggcatt atcggtgctg ttcttatgtt atccgcaagc cagtcctggg cagtgacatt
      721 aaaactgagt cataatcagg ataagtctca tcctgttcat aaagcgatgg agttctttgc
      781 gaaaaagagc aaagagtact ctaacggtga tattactatt cgtatttatc caaatggaac
      841 attgggtact caacgagaaa caatggagct gattcgttct ggcgctattc cactggtaaa
      901 aaccaatgcg gcagaaatgg aagcatttga aaattcctat aaattattta gcctgcctta
      961 tttgttccgc gatcgtgatc attattatca ggtcatgcag ggcgatatcg ggagaaaaat
     1021 cctcgactca acgaaaagca aaggttattt cgggctgact ttttatgatg gaggcgcccg
     1081 cagtttctat ggcaataaac cagtactgaa accagacgat ctgaaaggca tgaaagtccg
     1141 tgtccagcca agccctggcg cagttgaaat gatcaaagtc atgggcggta acccgacgcc
     1201 actggattac ggcgagttgt atacagcctt acagcagggt gtggtcgata tggcagaaaa
     1261 cagcgtgatg gcgctgacca ccatgcgtca cggtgaagtg gcaaaatcct tcagccttga
     1321 cgaacacact atggttcccg atgtggttct gatgagcaat gctgcgtttg ataaacttag
     1381 cccggaaaat caggaagtta tattaaaagc agctaaagaa tcaatgagct acatgaaaga
     1441 cttgtggagc gaggaagaga aacaagaatt tgcaaaactg gataaaatgg gcgtgaaagt
     1501 ctaccaggta gataaagctc cgtttatcga gaaagtacag ccaatgtacg caaacttcgc
     1561 taaggataac ccagcccttg ccccaatgct ggctgatatt caggcggcta agtaattatc
     1621 ttcgtagggc tgcttattgt ggcccttatc gatatctgca agtgctcgct acaaaagcaa
     1681 aacgccgacc aatggtcggc gtttttttac gtctcgttga ataacgaatt attcagctac
     1741 tacgtttacg atcactttcg cgaatacttc gctgtgaacc tggaagctca cttcgtgttc
     1801 gccagtggta cgcagaacgc cgttcggcag acgaacttcg ctcttagcca cttcaacgcc
     1861 agctgcagtt acagcgtcag cgatgtcgcg agtaccgatg gaaccgaaca gtttaccttc
     1921 gtcgccagct ttagacgcga tggtaacagt ttccagtgca ttgattttct cagcgcgagc
     1981 attagcagct gccagaactt cagccagttt agcttccagt tcagcgcgac gtgcttcgaa
     2041 gaattcaatg tttttcttgg tagctggaac agctttaccc tgcggtacca ggaagttacg
     2101 agcatagccc gctttaacgt ttacctgatc acccaggcta cccaggtttg ctactttatc
     2161 aagcagaata acttgcatta ccttatcctc tcaaagtcgt attaatggac cgtgaccgat
     2221 tactgatggc gatcagtgta cggcagcagg gacaggtagc gagcgcgttt gatagcgcga
     2281 gccagctgac gctggtattt tgcacgggta ccggtgatac ggcttgggac aatcttaccg
     2341 ctttcggtga tgtagttttt cagcgtagcg atatctttat agtcgatctc ttgaacgcct
     2401 tccgcggtga aacggcagaa cttgcgacga cggaaataac gtgccatatg gctagtctcc
     2461 agaatctatc aattcaatct gctcggcatg caaaaccatt ttgctcagtc cgttctttgc
     2521 cttgtggcat gaaatgaacc cctgaacggt tatgcgactg ccgaccgtta tactgtgagt
     2581 aatggcctgg ttttcgtgtc cgctaacaat aacgggcatt tgacaccatg cctgccggtg
     2641 aaagccggct tcctcctgca cagaacgatg ctcaagcacg aactggcagt gaggaattcc
     2701 tgatggactg acctttcgaa ggggagtcct gcacacggtg ccggacaaca ccagacggtt
     2761 ggtcatcaga aattactctt cagaatcccc agcttcagca tcatcagcgg tttcgtttgc
     2821 gaaatcatcg cgacgctcac ggcgctcgtc tttcgcttta accatcggag atgcttcggt
     2881 aacagcgtgc ttggtacgca taaccatgct gcggataacg gcatcgttga agcggaaggt
     2941 agtttccagc tcatcgatca cttcctgcgg agcttcaacg ttcatcagaa cgtagtgtgc
     3001 tttgtgcagt ttgttgatcg ggtaagccag ctgacggcgg ccccagtctt ccagacggtg
     3061 gatcttgcct tctgcaccag tgatggcagc agtgtagcgc tcgatcatgc ccggaacctg
     3121 ttcgctctgg tcaggatgga ccataaaaac gatttcgtaa tgacgcatcg aattgctcct
     3181 tacggattat tcagcctcct gtctgggtca gccgaatccc ggggaggcaa ggaacgtgtt
     3241 aaaggtcggc tgaaaaatga cgcgttatag tacttgtatg ccccgctaaa ctcaaggcca
     3301 gccgacaaat aaatctcatc tttcgctcaa aagaactaac tccctgattt gaagttcatc
     3361 acattccgtc acagtgtttt ttcgaacaac tgcatcagaa atggtcacca tcaacttctt
     3421 tatcattgat taaaattaaa tcagaagatc atcacagagg agagcacaca aagtcagccg
     3481 tcgaggaggt atctatgttc agtcgtgttt tagcccttct ggctgtgctt ttgctaagtg
     3541 caaatacatg ggcagccatt gaaatcaata accaccaggc cagaaatatg gacgatgtgc
     3601 aaagcttagg cgtgatttat atcaatcata atttcgccac tgaaagtgaa gcacgtcagg
     3661 cattaaatga agaaacagat gcgcagggcg caacgtacta ccacgtaatt ctgatgcggg
     3721 aaccggggag taacggcaat atgcacgcca gcgcggatat ttatcgctag caccaggtat
     3781 aaccaacgaa acattgccat agtttgcttt gcccccttcg cagggggctt tttttgaaaa
     3841 acggctgcgg aattagacca gttatctccc gaagaaggaa atttccgcag cgcgtgtttt
     3901 acttctgccc gtaataagcg ttaggaccat gcttacgcat gaagtgttta ttcatcagga
     3961 agctggcgat gtgattgagt tgtggattaa tactgcgcgc aatccacgcc attttcgcca
     4021 cttcttccat cactaccgcg ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg
     4081 gtccgtgctg atacaccaca attcccggcg tatgcagcgg ctcggcgttg cccagcgttt
     4141 cgataatcac tttgccagtg ttcagttcat actcaccctg cacctcttct tcgcttaatc
     4201 cacgcgtaca cgggatgtcg ccaaagaagt agtcggcatg cgtagtgcct aacgccggga
     4261 tcgccagccc cgcctgcgcc catgcggtag catgagtgga gtgggtatgg acaatgccgc
     4321 caagcgacgg ataacgacgg tagagttcaa gatgcgtcgc ggtgtcggaa gacggacgat
     4381 attccccttc caccacctta ccgctcatat cgaccaccac catatcgtct gctttcatgg
     4441 tttcgtaggc gacgccgctg ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc
     4501 tgacgttgcc ccaggtaaag gtcaccagcc catagcgcgg cagatccatg ttggcttcaa
     4561 atacctgctg ttttagcttt tgcattatgc cgcctccacc atgcccgctt tcgccatgcg
     4621 cgctttcacc caatcacgcg ctttcgccac ttctgccgcc gggtcttccg ccgtttcgct
     4681 ccacatctca atcaggtacg gcccgcaata gccactctgt ttgagcgttt cgaaacaacg
     4741 ttcgaaatcc actacacctt cgccaaacgg cacgtttttg aagacgccag gtttggtgtc
     4801 tttcacatgt accgcgacga tatgcccgat tccggcctgc aactccatct gcacatcgtt
     4861 gtcccacgcc gacaggttgc cgatatccgg gtagagctgg aaccacgggt tgttgagata
     4921 gtgcgcgtaa cccagcgcct tgctgatgga gttcatcaac ggataatcca tgatctccat
     4981 cgccagcgtc acctgcgcgc ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa
     5041 acgacgacgc gtttcgttat tggcttcctg atagtaaacg tcatagcccg ccagctggat
     5101 cacgcgaatg ccgacgtcct gggcgaactg gatagcttta cgcataatct ccagcccctg
     5161 cgcccgcacc gcgtcatctt cactgcccag cgggaaacga cgatgagcag aaaggcacat
     5221 ggacggcacg cgcacgccgg tttcaacaat cgcattcacc agcgccagac gctgctcgcg
     5281 gctccagtcg aggcgcgaca ggcgctcgtc agtttcatct accgacatct cgacaaaatc
     5341 gaagcccaac gtttttgcca gtcgcaggcg ttccagccag cactccccgg cggggagcgc
     5401 tttttcatag atgccaagcg ggatttgttt ggacaacata tccgctcctt agccccacag
     5461 ttcggcgatg gaacgtttga actgacgcgc ggcttcaacc ggagaagcgg catcacggat
     5521 gctgcgaccg gcgataaaca catgaatcgg aatacctttg aacagcggca gatcttccag
     5581 cgccaggcca ccggtgacgg tgactttgaa gcccatatcg gaaagacgtt tgatcgcggt
     5641 aatgtccgct tcgccccacg ctacgcctgc ggcctgcgcg tcacggctgc ggtgataaac
     5701 cacctgctga atgcctgcgt cacgccactg ctgcgcctgt tcccaggtcc agtagccggt
     5761 caattcgatc tgcacgtcgc cgttaaactc ttttgctacg tccagcgcgc ctttggcggt
     5821 gttgatatcc gcacagcaaa ttaccgtcac ccagtcagcg ttggcttcga agcacatacg
     5881 cgaaaggatt ttgcccgcat cagcaatttt agcgtctgcc agtacgattt tgtgcgggta
     5941 gagcgctttc aggtcacgaa ccgcacgcac gccttcgccc acgcacagaa tggtacccac
     6001 ttcgataatg tcgacttctt cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt
     6061 ctggttgtcc agcgcgactt gcaacatcgg taatgacatc ttcaattcct tcttaagctg
     6121 ccgcgttggt gcggtcaatg agatccagta cttcctgctc ggtacggcag gcacgtaaac
     6181 ggtcgaaatt ttcttcatct tcaaacaggt tgacgatctg catgatgccc acttcctggt
     6241 gcgtgttggc atcgaccgcc gccatggtga tgaggatatc caccgggtcg ttatcttcgt
     6301 ggttgaactc cagcggcttt ttcagcgtca ccagcgcaaa accggttttc ttaacgccct
     6361 cttccgggcg accgtgcggc attgccaggc ctggtgcgag cacaaagtac ggaccgtgct
     6421 gttcaaccgc atccagaatc gcctggtagt aacgcggctc gacaacatcc gccgccacca
     6481 gcaggtcaac gccgattttt actgcgtcct gccatgtctc agcttcagcc tgcaggcgga
     6541 tggatttatt ctccgccagc gaatcacgta atttcatggc gcgtccttac ttcacatcct
     6601 gcgggaaatg cgctttgatc acttccagca gtttcgggcc aaagtcggca ggagagagca
     6661 tgttgcgcac gccaaccacg tatttgttgc cggtcacgat gatttcgccc gcaatgtgcg
     6721 tagaagcgat gatgatatcc gcgccgctca actcgctttt gtattcgcca accgcgcagc
     6781 tatttaccgt gtggtcaatg tttgattggg ttaaaaactg gtccactttc atcttcatga
     6841 tcatggaact gccttgtccg ttgccacaca cagccagaat acgtacggtc ataatcaaaa
     6901 ctccttatta agcagactgt tctgccagtt gtttttctgc atcttcttct gcgcgcagtg
     6961 cgcggccagc gaagaacata taagccagtg caattacaat gatgacggcc ataaaggcga
     7021 taccgatgga gaagaagccc tgcatcatcg gcggtgccag aatcgaccag tccgccatgc
     7081 ccatccaggc gctcataccg gtgagtttca ccgcccagac gcagccaaag atttcaatca
     7141 tccccatcac cagacatatc ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca
     7201 cgccgatggt ggcgttggag aagaacatcg ggataaagcc gggaataatc aggatcgagg
     7261 aaccgcaggc gaccaggatg ccaaccgcaa tcagctgacc gatggtgccc cacataaagc
     7321 cccaaaccac ggcgttcggc gcgaagctat agatagccgc acagtcaatc gccagaaccg
     7381 caccagggat caggcgctga gagataccgt taaatgcttc agagagttcc gccacaaaca
     7441 tgcgcacgcc ctgagtgatg atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga
     7501 tgtacaccgt ccagttcact ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga
     7561 gcagaatggc accaaagaag atggtcatca caatcgccgt ggagacgatg ttgtcgtgga
     7621 aaatattcag ccagcctggc agtttgaggt cttcaacgct ttcctctttt ttgccgagga
     7681 acggcgcaac tttataggca atccatgatg caaactgttg ctggtgaccg atggagaaac
     7741 cgcagccgtc cgtcacttcc tgagtcggct tgtacatcat gttggaggta atgccccagt
     7801 agagcgaaac cagaatcgcc gtgcagataa tggttgtcca catggagtag ccgaaaataa
     7861 acaacgtaac ggcaatcagc cccgcctgct ggaacatgat atgaccagtc agcatgatgg
     7921 tacgaatacc ggtaatgcga cgcagcagca cgtaacagat gttcagcgcc agcgctaaca
     7981 acaccgcgta acctacccag ctataggcat cgcccatgcg gtcgatggtt gccatcatcg
     8041 acgcgtaagt atcggaaatt gcgccgttaa tgccgtagac ttcggacatt ttcgccacca
     8101 ccggtttgaa ggtgctggtg aggatgccgg accctgcctg caacaacatg aaaccaatta
     8161 tggttttaat cgtgccttta ataataacgc tgacactttt gcgcagtagg atgtagccca
     8221 gacaggtcac aatacccagc aacaacgggg cattggtcat gacctggtta aaaaacacgg
     8281 taaagatgtt gtagaggatc tccataacga tctcctgaag aagaggtaac cgggtattcc
     8341 acacacccgg aatgttgtgc actcactcta attttcaaaa gtaatcacaa caagattatt
     8401 tatgattaaa tgtgacgcga cccgcaaata attccacatg gatagaatgg gattcacaca
     8461 acgaaacaac tattcattta aattcattta gttaattgaa ttacacattc cattaaatct
     8521 ttccacactc aaaatcaaca accacttccc tggcaaaaag tcaaaaaatg gtcattgcct
     8581 tcctgaaaaa atagtgtcag aattaatcaa aaccaatcac acattgattc gaattgaaca
     8641 atcatgaagg taaatggcga tgagtaaagt gaaaagtatc acccgtgaat cctggatcct
     8701 gagcactttc ccggagtggg gtagctggtt gaatgaagaa attgaacaag aacaggtcgc
     8761 tccaggcaca tttgcgatgt ggtggcttgg ctgcaccggg atctggttga aatcggaagg
     8821 tggcaccaac gtttgcgttg atttctggtg cggcactggc aaacaaagtc acggtaaccc
     8881 gttaatgaaa cagggtcacc agatgcagcg catggctggc gtgaaaaaac tgcagccaaa
     8941 cctgcgcacc accccgtttg ttcttgatcc gtttgcgatt cgccagatcg acgcggtact
     9001 ggcgactcac gatcacaacg atcatatcga cgttaacgtt gctgctgccg tgatgcagaa
     9061 ctgtgctgat gacgtaccgt tcattggacc gaaaacctgt gtggatttgt ggattggctg
     9121 gggcgtaccg aaagagcgtt gcatcgtggt caaaccgggc gatgtagtaa aagtgaaaga
     9181 cattgaaatt catgcgcttg acgctttcga ccgtactgca ctgatcaccc tgcctgccga
     9241 tcaaaaagcg gctggcgtac tgccagatgg catggacgat cgcgcagtaa actacctgtt
     9301 caaaacccct ggcggttccc tatatcacag tggcgattcc cactactcta actattatgc
     9361 gaagcacggt aacgaacatc agatcgatgt ggcgttagga tcgtatggtg agaacccgcg
     9421 cggtattacc gacaaaatga ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc
     9481 gaaagtggtt atcccgttcc accacgacat ctggtcaaac ttccaggccg atccgcaaga
     9541 gatccgcgtg ctgtgggaga tgaaaaaaga tcgtctgaag tatggcttca agccgtttat
     9601 ctggcaggtt ggcggcaaat ttacctggcc gctggataaa gacaacttcg agtaccacta
     9661 tccgcgcggt ttcgatgatt gcttcactat tgaaccggat ctgccgttca agtcattcct
     9721 gtaatctgtc gtttataccg gatagcgtgg agaagttcgc gctatccggc gtagtatttc
     9781 ctggctattt caaatatcat ctaaaaaaat caaattttat cggaatagct catgactgaa
     9841 gcacaaagac atcaaatcct cctggaaatg ctcgcacaat tgggctttgt gaccgttgag
     9901 aaagtcgttg agcgtctggg aatttcgcct gccactgcgc gacgcgatat caataaactt
     9961 gacgaaagcg gcaaactgaa aaaagtgcgc aatggcgcag aagctattac ccaacagcgc
    10021 ccgcgctgga cgccgatgaa tctgcatcag gcgcagaatc acgatgaaaa agtacgtatc
    10081 gctaaagcgg cctcgcagct ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc
    10141 accgcgtttc tgcttgggcg ggaaatgtgt ggcaagccag tgcaaatcat cactaattat
    10201 ctaccgctgg caaattacct gatcgatcaa gaacatgaca gcgtgatcat tatgggcgga
    10261 cagtacaaca aaagtcagtc catcacttta agcccgcagg gcagcgaaaa cagtctctat
    10321 gccgggcact ggatgtttac cagcggcaaa ggactgacag cagaagggtt gtataaaacc
    10381 gatatgctga cggcaatggc agagcagaag atgctgagcg tggtagggaa actggtggta
    10441 ctggttgata gcagtaagat tggcgaacgc gcgggaatgc tttttagccg tgccgatcaa
    10501 attgatatgc tgatcaccgg caaaaatgct aacccggaaa tcctgcaaca actggaagcg
    10561 caaggggtca gcattctgcg tgtttaaaga tgctggcgga aaaatgtcac tgcagcatct
    10621 aacgcctcag gcgtaatgcg gtgacgcacg cctggctgcc atgaacaggt tagcagttta
    10681 tcccgtcccg tctcgcttaa ggcctgctgc aaacgtagtg attcgtcggt aggcacaacg
    10741 tcatcgtcga ggccatgcca cagcagtaga ggtctgtcac taagttgttc caggtggttt
    10801 gtcgcttccc actctgccag cggcgcgaca atgttattga attcattctg ctgtgctgtc
    10861 gtttcaggta tcagcggtgg aaacagtgaa cgggcgagtg atgtaaaata gcccgatccc
    10921 atcatgctgg cggtacatct caccgtgggg tggcgagcgg taatccccag tgccgtcatc
    10981 gcgcccatcg acgcaccacc gactgccaga cggtcatcaa gcagccagtt ttcttcggct
    11041 attgccgcac gtaaagtagt gaattcctgc atactttgta gcaagatttg ccagaattga
    11101 tttaaccgcc gcgctgcgtc accactaaaa cggctaccgt gatcgggcgc atccggcatg
    11161 atcactcgca aaccagcctg cgccagcgca acggcaaaat agctatacac cagactggat
    11221 gaagtaaagc cgtgataaaa aattacgcac ggtaacgggg tatctttttg cccgacagga
    11281 taagcatgaa gaacgggaat atctgccagc tcgcgtgatt ctatttcaat catctgtctc
    11341 tcctttttgt ctggtatgac atgaagaaat ccttagcaca agtttctgct ggcggtatta
    11401 gttcataatg ttgagatgtg ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac
    11461 caggtaacaa ttcgggaaca ttcccccaaa gttaaattaa taaggcacta cactatggtt
    11521 agcaggaaac gtaatagcgt tatttaccgg tttgccagtt tattattggt gttgatgtta
    11581 agtgcctgta gcgcactgca aggtacgcca cagccagcac cgccagtaac ggaccatccg
    11641 caagagattc gccgcgacca gacgcaaggg ttacagcgaa taggcagcgt aagcacgatg
    11701 gttcggggtt ctccggatga cgcattagca gaaatcagag cgaaagctgt cgctgcaaaa
    11761 gctgattatt acgttgtcgt catggtggac gaaaccatcg tgacaggaca gtggtattca
    11821 caagccattt tgtaccgtaa ataaatcaga caacttatat agtcagcttt acattgcttt
    11881 gcgtccctat gcgtttacct gtgcacaata aattaccggc tggaagcctg ggcgtttatt
    11941 accgcgatgg aatgccctgc aacatgtggg gaaacgaaaa atggagctga cgatgaaaca
    12001 attacttgcc tcaccctcgc tgcaattagt gacttatcct gcgagcgcca cggcacagtc
    12061 tgcagaattc gccagtgctg actgcgtgac gggcctgaat gaaataggtc agatctcagt
    12121 tagcaatatt tcaggggatc cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga
    12181 acaaggtgcg tcatggtacc gcattattac catgtacgaa gaccagcagc ctgataactg
    12241 gcgcgtacag gcgatactgt atgcatgagt gtcatctaca gcaaatgaac aatgccggat
    12301 gcggcgcgag cgccttgtcc ggcctacaat tttcacatat ttcatttagt taatcgaaac
    12361 cagcgtcgca tcagtcgtat acgcttacac acacactccc cccgtcgccc gcagcaataa
    12421 atcattctgg atctgctctg acaaccgtac gccgccgcgc gtatctaaca tcacctgaca
    12481 ccacgcctgc gccattggcg gagaagcata tttcaacatt tgcgcaccgc agcccagcag
    12541 gaatagctga tgagtaatct ctcgcctcag ttcttcagct ggcttacgca gctgctgctg
    12601 taaacgacga accgcgcgat caaaatagcg atcctgccct ttcacttcca caaacgcttc
    12661 cgacaataag tcgtatacgc ccgcttgctt attgagaacg cgcagcacat ccaggcacat
    12721 aatattgccg gaaccttccc aaatactgtt taccggcatc tcccggtaaa gccgcggcag
    12781 ctcgctctcc tcgcaataac caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg
    12841 catacctctt ttgcagatca caaattttgc cgcaggcgta aataaacgcg cccacagggc
    12901 ttctttggca tcggcacgcc ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg
    12961 cccttcaagc tgaagtgcca tgcgacttaa gacatgacgc ataaggggct gttggatcaa
    13021 tggattacca aaaacatggc gttgatgtgc atgataaatc gccagcgaaa atgcacggcg
    13081 catcatggca tggctaccca gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat
    13141 cagacgaatt ccttcccctt cctgccccaa caaccagcca atggcatcct gaaactccac
    13201 ttcgcagctg gcgttagagc gattacccag cttatctttc agccgctcga ggcgaatcgc
    13261 gttgcgttgc ccgtcaggca aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt
    13321 ctgcgccagc accagatgcg catcgctttg cggcaccgag aaaaaccatt tatgccccac
    13381 cagccgataa gagccatctt ccaggcgctc tgcgcgggtg gtgttgctca tgacatcgga
    13441 accgccctgc ttttccgtca ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg
    13501 caataagtga gaatcgtagc gatcgctcag cagcggcgtg gtccagtcct gaaacggcgc
    13561 gggtaacatc tgtaacagca atggcgtggc ggcaaaggtc atggttatcg gacataacga
    13621 ccctgcctca acctgtgcgt gcaacataaa acgcgccgcg cgcgccacaa atgcgccgga
    13681 gcgagcgtct tcttcccagg caagattgtg cacccgattg gtacatagcg cctgcatcag
    13741 caggtgccag gcggggtgaa aacgcacatc gtccagacgg cgtccttgcg catcgtagcg
    13801 caataattca ggcggattca cattcgccag ccgccccagt tcaagggatt cagccgttcc
    13861 taactgctga ccaatactgg ctagaaaatc gctatcccag ccagcacctt cacgcgttac
    13921 cgcttcgcag agcgcgccat cagacaggta taagttgctg ttatttaatg gtataggttg
    13981 attaaaaacg gtgtgagttt gccagtgcac tgtgtctccc tccatcaatg gcagtcacca
    14041 ttagtatggt cactgccatg gattcatgac agaaagagat tgctaaaaca ttcttatcca
    14101 gcaatgtaat gcggaacgaa acgtgaggtg tctttggtga tcagtgtgtt atcttcacgg
    14161 atccccattc cgcacgcttc atcatcgaca atccagctac cgatgagtgt gtagctatcg
    14221 ccaaatcgcg gcagaggttg aaacgcctgg tagatcatcg gttcatcggc gtaatcacca
    14281 tcagcgtggt caacgacgtt attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgc
    14341 gagtagatcg gtttacgcac atagctttcg ccagcggcaa tctgcgattt ttcaccctca
    14401 aaccaggatg caagaaggtt aggatgacca gggaagcagc ggcattagcc ctttattact
    14461 caagatactt ttccataacg gctccaccca ttgctcgcga cgcttgcaca gcagcggacc
    14521 gttatcgtca cgcatcatcc actccagcgg atacagctta aatgcacgct ggatgacatt
    14581 atcatcaaga tcggtcagta caccgccgac gcccaaaccg agatcttcaa tgtagataaa
    14641 ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg tagtcctgtc
    14701 ttcatcggtg tcctgacagc agcagaaata aaacggctcc cggctataaa gctcgctaaa
    14761 gcgtgaaatc aggcgttcct gaatggcatt gtactgatcg gcatcacgcg gaataatgcc
    14821 gctgcgccgg gcatcctcca gccacaacca ctggaaataa gccgactcat acaacgaggt
    14881 cggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc aggcaaaatc
    14941 catccggcca tacagcgaag gatcgcgtgc gcgccagctt tcggcgatca catcccagta
    15001 caacggcggg atcgccagtt gcgtcaggat ctcttcgtct ttaaccgcgc gatccaccac
    15061 ctcaaggcac atctgatgca gttccgcagt cggtttttcg atctgctctt caatctggcg
    15121 cagagtaaaa cggtaagccc gactctcgtc ccaatagatt tcattgtcga tgatatgaaa
    15181 gtcgaaaccg ttgttagcgg cgatctggtc cagatcccgt cgcacaggaa cgttgtgtct
    15241 cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata gccgccgcgc
    15301 gaaacggttg acgctttttt ggtggtaaag ccgcctgaag agtaagactc ttttttgcca
    15361 gaaccggagc gccaggagta atcaccggaa gtgttgcgcc agacggggcg ggaagcaaat
    15421 gaggatccgc cgctgttata gacgaacggc tcatcgcgat ctttacgaat aactcggctt
    15481 aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cattgtcgta gtagcaattt
    15541 tcgtacttag actgacaatt ctgctgagtc atattcttcg gaacatcggc ataaaatgcc
    15601 gctttggcgt tgttccagcc acgcgcgcaa atgtcggcat tattaccgtc atcaatacaa
    15661 tcctgcactg tcgaataaaa cgttccgtcg ccgtcgttat cgacatcgcc actttcactc
    15721 catcctttga ctattaaaaa aacggcaccg cccattagca ccaacgttaa gtatttttgt
    15781 gcgtagcgat tgcggcgcgg ctcaaaagga ttatgcggtc ttccaatgcg actgatcgct
    15841 ccgtgaccaa ttttactgtt atttcttgat ttgcgttttc tggccatgat cgtccattac
    15901 catgtcatac aagctgcgtt aaaaatacca ccagccagtg cggcggttcc cataaacatt
    15961 cctgctgcgg tattgtgatt aataattttt tcgcttaatg cgggcatata aagtctgaca
    16021 ccagcaaaaa cgagtaactg aatcaccagt gcaatcccgc cccaggcgaa atagtctgga
    16081 atactcaccg cattgattgc cgcactggat aaggggatca cgtaacccaa caatgtaccg
    16141 ctgaatgcca gtgacgccgc ggtattattg tttttgatta attgccattc gttgtgcggt
    16201 gtaattttgg agtagataaa cagaaaaata atcaccatag ccacgccaat aaaaaaatag
    16261 gcactaaagg caagaagtga atccagtata tgcattgtaa acgtccttgt aattaaccaa
    16321 taatatgaag tgatgtcagc gggatatcga cacccaacgc gcgcgaaaat aaccactctg
    16381 gctcgccgag atcgttaaaa gattcctcac catttaacag caaatattcg taagtatctt
    16441 cggttacttg gcgttggtag cccatggtaa aattatggac ctcccatttg gcatggtttt
    16501 gattttctac tttttccagc atgtagacgg gttcgatatt tcccggttct tcgctgttaa
    16561 aaaatcgctg ccagcgtttt tcctgccagt ttaagatcat tgcccccatc actttggcgt
    16621 tgatcgcctc gcgccagtga ctctctttac tgataccgta gctctcttca tagacaaaga
    16681 gcttgatatc atcaatgtca tcaatatctt cgccgccggt ggtattgatt tgcagaaatt
    16741 catcgcctga agtgtagtag cggaaaatct gactaccgcc gcccagatcg atgtggctga
    16801 cagcggctac cgtaaattcc tcacccggca gcgcaatcag caattcatct tccagcaaac
    16861 gaaacgctaa cgtgtcgagc gtaaagccac tattgagatg aagtcccagc ggaccgcgag
    16921 cgatagcagg cttattatcc ttgccaaaca gacgctggaa aaaaccagac atattttatc
    16981 cttaaaatag ttgccagcct tttagcgagg ctggcaaaaa aattactcgc cctgttggcg
    17041 ttgcagtctt gctaatacat cctgggcgct acttttattg ctaccgccaa ttccggcttc
    17101 cgccagcttt tcatcaagat cgcgaccgtc tgcgactttt tccaactgtg cagcagcatc
    17161 caggcgagcc tgacgttcgg cctgacgcgt ttgcaggcgt tttaaggatt ctgccgctgt
    17221 cgaaacgctg gaagatgcgc caacggtaga ggttgttaca gcctgttgtg cacgctgcat
    17281 ggcttcagtg gctttaacga cttccatttg ctgctcaaac tgagcgatac gctgcgctgt
    17341 cgctgtaact gctttttcca cgccatcacg ggagacttcc agattcgaca acacttgctc
    17401 ttcagcggta atgagattct caaggcgcgc gatttcttca gcaacttcgt taatcaacga
    17461 cggattaacg ttcttgctta acgcttccag cgcacgggct tccagactgg ctttgcgctc
    17521 acgtaaatct ttcagcttat cgtgacttaa tttcacccgc gccagcagat caacgcgaga
    17581 ttttccggct ttatcgagtt cagctttagc gtcacgaata tgctgttcca gcatgcgcac
    17641 gccctgggtt tcttcaatgg attcttccgc ctgggagata aacgatttcc ccagcgtaaa
    17701 taaacttttt aaaattccca taaatacccg tccttgtaat tagtgtgaat attcttctgt
    17761 aatttcagcc agatccaatg cgttatctac cagcgaggtt atctcaagca ggatatcttc
    17821 aagagaagat ttaagcgata acgccccgaa aacaatgtaa tactcttcct gttgcacgct
    17881 ggagattcct accgatgaca acggcatcat cttctgattt cttaataaga aggtattaaa
    17941 ttcatctgga ttgctgatac tacttaccgg acaaataaag gtttcgataa tcatttggcg
    18001 ggaagtaaaa agaatattga tttgcaaatc gccataatca ttcattttaa taattaatgc
    18061 gctctcgcta tttgtcacat caatattttg ttcaggtaca gtttgcagcg ccgtcgccag
    18121 cgccaacgga ttccatgtca tatattattc catatagatt tagtttaaat attaataaaa
    18181 tgaatatttg cagtacgtaa ttatcttacc agctatagac aaaaaaaaac catccaaatc
    18241 tggatggctt ttcataattc agacgaacta gctgcgctga cgaaccgctt caaataagca
    18301 aattccggtt gcaaccgaaa cgttcaggga agaaacactt cctgccattg ggatgctgat
    18361 caactcatcg caatgttcac gggtcaggcg acgcatacct tcaccttccg cgcccatcac
    18421 cagcgccagg cgtccggtca ttttgctctg atagagtgta tgatccgcct cgcctgccgt
    18481 accgacgatc cagatattct cttcctgcaa catacgcatg gtgcgcgcaa ggttagtcac
    18541 ccgaatcagt ggaacgcttt ctgccgcgcc gcaggctact tttttcgccg tagcgttgag
    18601 ctgtgcggag cgatctttcg gcacaatcac cgcgtgaacg ccagcagcgt ccgcgctacg
    18661 caggcacgcg ccgaggttgt gcggatcggt tacaccgtcg aggatcagca ggaacggttg
    18721 atcgagcgaa gcgatcagat ccggcagatc gttttcctga tactgacgtc ctggcttcac
    18781 gcgggcgata atgccctgat gcacggcacc gtcgcttttc tcgtcgagat attggcggtt
    18841 tgccaactgg ataaccacgc cctgagactc aagggcgtga atcagcggta acagacgttt
    18901 atcttcacgg ccttttaaaa taaagacttc ctgaaaacgt tcaggggcgc gctccagcag
    18961 ggcctgcact gcgtggatgc cgtaaatcat ttcgctcatt aatgtactcg ttgtttacgt
    19021 tttgcctgat gcactacgtt tatccggcct acatgatctc tgcaatatat tgaatttgcg
    19081 tgattatgta ggccggataa ggcgttcacg ccgcatccgg catgaacaaa gcgcattttg
    19141 tcagcaatct aaccctcttc ttttaaagag ggtattgatc actctgccac ttttttcttc
    19201 gccgcacgct tcgctttggt cgctgcggct attttctgcg ttttcgccga tggctttttc
    19261 gcttttctcg cgtctttctt cgccgctttc ggcttcgttt ttttctcacc gcggaaggcg
    19321 ctgtctggct caaagtttac ctttttaccg acctgacgac gcttgccgcc ttttttgcct
    19381 gcatcgcctt ttttcgcttt ctcgcgcgcc gttttaccga cgttgcgcgg tgcgcgttca
    19441 ctggagatca gactaaagtc gattttgcgc tcgtccatat taaccgcttc gactcgaact
    19501 tccacgcgat cgcccaggcg ataagtctgg ccgctggatt cccccatcag gcgttgccct
    19561 acctggtcaa agcgatagta gtcattgtcc agcgaagaga catggaccag accatcaatg
    19621 aacaagtcgt ccagacggac gaagaagcca aagccagtga cgctggaaat tacgccttta
    19681 aagacgttac ctacctggtc gagcatgaag tcacacttca gccagtcagc gacatcgcgc
    19741 gttgcttcgt cggcacgacg ttccgccatc gaacagtgct gacccagttg caacatctct
    19801 tccatcgaat aatggtagcc gccggtttca gtggtgttgc cctgatgccc ctgctctttc
    19861 gccagcagat acttaatggc gcggtgcagc gtcaggtcag gataacgacg aatcggcgaa
    19921 gtaaagtgcg cataggactg caatgccagg ccaaagtgac cacggttttc tggatcgtaa
    19981 atcgcctgtt tcatcgagcg cagcagcatg gtttgcagca tttctgcatc aggacggtcg
    20041 gcaaccgact ccagcagttc cgcgtagtca cgcggttccg gcttgttacc acccggcagc
    20101 tccagcccca gctccgccag cactgaacgg aaagaggtaa tcgcttcggt gctcggcttg
    20161 tcgtgaatac ggaacagtgc cggttctttc gctttctcaa cgaaacgcgc cgccgagata
    20221 ttcgccagaa tcatgcactc ttcaattaat ttgtgcgcgt cgttacgctg ggtctgttcg
    20281 atacgttcaa tacggcgttc agcgttgaaa atgaacttcg cttcttcgct ctcaaatgag
    20341 atcccaccgc gttcttcacg ggctttatcc agcactttat agaggttatg caactcttcg
    20401 agatgcttaa ccagcggggc gtactgctcg cgcagatcct gatcgccctg cagaatatgc
    20461 cagactttgg tgtaggtcag acgcgcgtga gagctcatca ccgcttcgta gaatttgtag
    20521 cccgtcaggc ggcctttcga cgaaaccgtc atctcgcaca ccatacacag gcggtctacc
    20581 tgcgggttga gcgaacacag gccgttagag agcacttccg gcagcatcgg gataacctgc
    20641 gaagggaagt acaccgacgt gccacggtta cgcgcttctc tgtccagcgg cgttggcgga
    20701 cgcacatagt agctgacgtc ggcaatcgcg acccataaac gccagccgcc gccgcgtttt
    20761 ttctcgcagt aaactgcatc gtcaaagtca cgggcgtctt cgccatcaat ggtgaccagc
    20821 ggtaaatcgc gcagatcaac acggcccgct tttgcttctt ccggcacttc ttctttcagc
    20881 ccggcaacct gttgctcaac agcctgcggc cagatgtacg gaatttcatg ggtacgcaga
    20941 gcgatatcaa ccgccatgcc ggtgcccata ttgtcgccca gcacttcgac gattttaccc
    21001 accgctttgg tgcggcgagt cggacgctga gtcagttcga ctacgaccac aaagcccatc
    21061 cgcgcgccca tgatctgatc gggcgggatt aagatatcga agctcagacg gctatcgtca
    21121 ggaaccacaa agccgacgcc cgcttcagta aagtagcgac caacaatctg gctggttttt
    21181 ggcaccagta cgcggacaat acgcgcttca cgacgacctt tacggtcagc gcccagcggc
    21241 tgcgccagca cctgatcgcc atgaatgcag gttttcatct gctcgctgga gagatacaaa
    21301 tcatctttac gcccttcaac ccgcagaaag ccgtagccat cacggtggcc aataacggta
    21361 cctttcacca ggtcgaggcg ttccggcagc gcatagcact gacgacgagt gaagaccagt
    21421 tgaccatcgc gctccatcgc gcgcaggcgg cgacgcaggc cttcaagctg ctcttcgcct
    21481 tcaatgtgca gttctaccgc cagctcatca cggctggccg gtttttcacg tttggttaaa
    21541 tgttcgagga taaattcccg actagggatg ggattcgcgt atttttcagc ttcgcgttcc
    21601 tggaaaggat cttgtgacat ctcggttcct ccgttgtcat ctctgatgaa gattttcgtc
    21661 actccaccag caataattta taaagcggtt gattctcttc aaccaaatcg gcaagcgtgt
    21721 agttatccag ttccgtaaga aaactttgca cggccttaga aagcgcctgt ttcaaccgac
    21781 aggcaggtgt aatgtggcaa aactcactgc tgcaattcac cagcgataag ggctccagct
    21841 cgcgcaccac atcaccaata cgtatcgcac tcgccgattt acccaggcga atgccgccat
    21901 tttttccacg aacagcagtc acgtagccgg cacgactaag ttgattgatt attttgacca
    21961 tatgattacg ggagacgccg tagacgtcag tcacttcaga aatactggtc atccgccctt
    22021 ctggcaatga cgccatgtag atcagcgcac gtaatccgta atcagtgaaa ctcgttaact
    22081 gcacatcaac ctcaaaaggg aaatcgggaa aaaatacatt tatattgatg ataaaccagc
    22141 cacaagctgt gtcgctaatt tatttcagtt cggggaagga aaaaagcgag gatttaacac
    22201 tgtgtcggat agcgtaatgg cttaaccgac acagcaatgt atcacactgt tagcccggca
    22261 ggcaaaatat ctgccaggcg taccagaatt acgcgtcgaa cggatcgcgc agaatcatgg
    22321 tttcagtacg atccggaccg gtagagatga tatcgatcgg cacaccagtc agctcttcaa
    22381 tacgcttgat gtagttcagc gccgcctgcg gcagaccgct acgatctttc acgccgaagg
    22441 tggattcaga ccagcccggc atggtttcgt aaatcggctc tacacctttc cagtcgtcag
    22501 ctgccagcgg agtggtagtc acttcgcgac catccggcat acggtaagcc acgcagagtt
    22561 taacctcttt caggccatcc agaacgtcca gtttagtcag gcagaagcca gacagggagt
    22621 tcagctgtac cgcacgacga acggcaacgg tgtccagcca gccggtacga cgacgacgcc
    22681 ccgtagttgc gccgaattcg ttaccctgct tgcagaggaa ctcgccagtt tcatcaaaca
    22741 gttcggtcgg gaacggacct gcacctacac gagtggagta agctttgagg atacccagaa
    22801 cgtaatcaac ataacgcggg cccaggccgg aaccggtcgc cacgccacca gcagtggtgt
    22861 tggaagaagt tacgtacgga taagtaccgt ggtcgatatc cagcagcgta ccctgcgcac
    22921 cttcaaacat gacgaaatcg ccacgctgac gcgcctggtc gagcaggtca gaaacgtcaa
    22981 ccaccataga agtcaggatg tcggcaacag ccatcgtatc atccagaact ttctggtaat
    23041 caaccgcttc agctttgtag tagttaacca actggaagtt gtgatattcc atcacttctt
    23101 tcagtttttc agcgaaggtt tctttgtcga aaaggtcgcc aacacgcaga ccgcgacgtg
    23161 ctactttatc ttcataagca ggcccgatac cacgaccggt ggtgccgatc gctttcgcgc
    23221 cacgcgcttt ctcacgcgcg ttatccagcg caacgtgata atcaaggatc agcggacatg
    23281 cttcagacag cagcagacgc tcacgaacgg ggatgccacg gtcttccagt tctttcatct
    23341 ctttcatcag cgcggccgga gacagcacaa caccgttacc gatgatgctg gttacattct
    23401 cgcggagaat acctgatgga ataagatgga gaacggtttt ttcaccgttg attacgagag
    23461 tatggcctgc gttgtgaccg ccctggtagc gtacaacata tttagcccgt tcagtcagaa
    23521 gatcgacgat cttaccttta ccttcgtcac cccattgggt gcccagtacg acgacgttgt
    23581 tacccatttt tcaaaatcac cgtttgctta aaaatggatt ctaccatcgc tttttcagag
    23641 ttacagcact ttttgcactc aaaaatgacc tcgatcagtc tgttttttgt tcagccaatc
    23701 gttttcctca acatgtagta gaccacaacg cccgcaacca caagtccacc gccaaaacga
    23761 cgtaaaatat tatcgggcaa attggtcatc gcagagatca tcttcttcca tgccttcggg
    23821 taaagcatcg gccctaaacc ttccagtacc aaaaccaggg caagcgccag ccagattgtc
    23881 gaattcattc attatcctta tagaaaaaga aaaccaccga catccctgag gatgcggtgg
    23941 ctttattgac ctgtaccgca gtcgttatat taacgcgttg cggaagtcgg cgtcttcatg
    24001 tagcggaaga aatcgctatc cgggctcatg accatcacgt cctgattgcc agagaagctg
    24061 ttctcataag cacgcaggct acggatgaat gcgtagaagt ccggatcttt actgaatgca
    24121 tcagcaaaca gtttggctgc ttcggcatcg ccttcaccac gcatgatgcg gccctgacgc
    24181 tcagcttctg ccagcgttct ggtcacttca tagtcggcag tcgcgcgcag tttttccgct
    24241 tcttcctgac cttgtgaacg gtgacgacgc gctaccgctt cacgctcggc gcgcatacgg
    24301 ttgtagatcg cttcagacac ttcggtcggc aggttgatct gcttgatacg cacatcgaca
    24361 acttcaatac ccagcgccgc catactgttc gggttgatga ccggaacttt gcccttcgtc
    24421 tcagccgtta cgcgctctgc cgcttcggca atggcgttat ccgctgccgg ggtagtaact
    24481 tcatcttctg tacccgcaga accggagttc agcgcgtcac gtacttcgag ggtcagacga
    24541 ccacgggaat cggtgacgat gtctttcacg tccaggcgac caatttcaga acgcagacgg
    24601 tcagagaact tacgtttcaa cagcacttcc gcttgcgaaa tgtcgccacc acccgttgcc
    24661 aggtagtaac ggctgaaatc gctgatgcgc catttgatgt aagaatcgac gatcaggtct
    24721 ttcttctctt tggtcacaaa gcggtcggcc tggttgtcca tggtctgaat acgtgcgtcg
    24781 agcattttca ccgtttcaat gaacggtatc ttgaaatgca gacccggctc ataaaccaga
    24841 ggtttgttgt catcgtcacg cagtacctta ccaaaacgca gcgtaatacc gcgctcacct
    24901 tctttgacga caaagacaga catgtaaagc actaccagca cgatgatgat aatcgcgata
    24961 actgacttac gcatcgttat tccccctgac gctggtagtc gttacgctgc gcgttggcgc
    25021 ggcgttggtc cataatattg ccctgactgg tggacgacgt gttgcttgct ccactggttg
    25081 tggaggaaga ggctggcggc aaacgcagca gattgctggc accgttatcg ctcttcgccg
    25141 caggggcgtt accacctttc agcatctggt ctaacggcag aaccatcagg ttgccacctt
    25201 tatcgttaac cagcactttg cgggtgttac ccaacacttt ttccatcgtc tcgatataca
    25261 gacgctcgcg agtaatttcc ggcgcggctt tatattccgg cagaagttta gcaaagcgcg
    25321 ccacttcacc ctgagcttcc aggatggtct gggccttgta cgcacgcgcc tcttcgagga
    25381 tacgttgcgc ctgaccgttc gcacgcggct gaacttcgtt ggtatacgct tctgcttcac
    25441 gaatgtattg ctgttcgttt tcacgcgcgg caatcgcatc gtcaaacgcc gcttttactt
    25501 cttccggcgg acgagcagcc tggaagttga cgtccagcag cgtgataccc atgtcatacg
    25561 gacggatagt ctcttccagt tcgcgctgag tatcgctacg aatcacggta cgaccttccg
    25621 tcagaatgcg gtccatggtg tatttaccga taactccacg cagggcgctg tcggtagcct
    25681 gacgcaggct gtcatccggg ctggtcacgc tatacagata tttttccgga ttggtgacgc
    25741 ggtactgcac gttcatctca acgcgcacta cgttctcgtc cgacgtcagc atcacaccag
    25801 aagcggccag ttcacgcacg gcttccacgt tcaccggttt gacttcgtcg ataaacgtcg
    25861 gtttccagtt cagacccggc tcaaccagat ggctgaattt accaaagcgt gttaccacgc
    25921 cgcgttcggc ttctttaatg gtatagaaac cactggccgc ccagataatg acaatcgctg
    25981 ccgctgcgat ggtaacgaca cgaccgccaa gctgcgggcg cgggccttgc gatgaactgc
    26041 caccgccaga tccggtgcct ttaccgccgc ccagaccacc gagctttttg ctcagtttgc
    26101 ggaagatatc atctaaatca ggtggccctt gatcgcgacc gcctttgttt ccatttccct
    26161 cagagttgcc gccaggtttg ctgcttcccc acgggtcgcg gtcttgtccg ttattaccgg
    26221 gctgattcca cgccatgttt gtgctccata tttgttatgc ggtgatccct gttgtctttc
    26281 gacgccagag gatatgactc cacgcttcag acgctacgcc gttagatcag gtaatcgatc
    26341 aacgccggtt cttgtttaca gaggcgacgc cagtcaacga tcggcatacg aacttgcaga
    26401 cttacgctgc cgtcctcctc catccactct ttttctattg cctgaagctg ataaaaacga
    26461 cttctcagac gcccttcctg cggtggcaga cgcaatgtat gctgcgccac ctcgccggaa
    26521 agccgctccg tcaaagcctg aaaaagctgt ggtatccccg ctccggtctg tgcggaaagc
    26581 cagacacgga tcggtttgtt ctcttcgtcc cgatcaatac gcggttcgaa atcttccagc
    26641 atatcgatct tgttcatcac cagcagggtt gggatctcgt gagcgtcgat ctcttcaaga
    26701 accgtattca ccgcttcgat gttttcttgt acacgcacat ccgccgcatc aatgacgtgc
    26761 agcagtaatg tggcttgccg cgtctcttgt aacgtggctt taaatgccgc caccagatcg
    26821 tgcggcaggt ggcgaataaa ccctacggta tctgcaagta cggtttcacc gacatctgca
    26881 acgtcaatac gccgcaacgt cgggtcgagg gtggcaaaca actggtctgc cgcgtagacc
    26941 cgcgcttcgg tgatgcgatt gaaaagggta gatttaccgg cgttggtata tcccaccagc
    27001 gaaacagtag gaacgtcggc tttgatacgc gattgccgcc cctgctcacg ctgcttttca
    27061 actctttcca ggcgtgactg tatctgcacg atgcgattac gcaacaaacg acggtcggtt
    27121 tcgagctggg tttcacccgg accacgcaaa cctatcccgc ctttctgtct ttcaaggtgg
    27181 gtccagccac gcaccaggcg cgtagccaga tggcgcagct gcgccagctc aacctgcaac
    27241 ttaccctcat gggtacgcgc acgttgggcg aaaatatcta aaataaggcc ggtgcggtcg
    27301 ataacacgac actcgcacaa acgctccagg ttacgctctt gcgccgggct cagggcatgg
    27361 tcaaaaagaa cgaccgaagc acccgtcgct ttgacagctt ccgcaatttc aactgcttta
    27421 ccttcaccta caaaatactt tgggtgcggc gctttacggc taccggtaat cacctgcaat
    27481 gcttcgacac cggcggaaga gaccagagat tcaaactcct ggaggtcttc catatctttg
    27541 tcttgcgtaa aatagatgtg taccagtacc gcctgctcac cagcatcata acggtcaaac
    27601 aagcgtataa ccctctaaat agatcagcgg ggaacgcagg atcgctggct ccccgtgtaa
    27661 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
    27721 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
    27781 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
    27841 gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg cagcttaata
    27901 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
    27961 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
    28021 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
    28081 gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt cactgtcaag
    28141 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
    28201 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
    28261 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
    28321 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    28381 aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    28441 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    28501 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    28561 cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    28621 ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
    28681 tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg ccgcggtgat
    28741 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    28801 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    28861 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    28921 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    28981 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    29041 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    29101 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    29161 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    29221 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    29281 aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg
    29341 ccagggcaga ctgcgctttt tctaatgccg atttttcttc ggcagaaact tttagccgca
    29401 acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat tgtgcctgac
    29461 gtagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg cgctccagca
    29521 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    29581 gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat cggcgcgggc gtttgcaaaa
    29641 gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg
    29701 cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc ggctcacgag
    29761 ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccgga atggaacgcg
    29821 gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    29881 tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt
    29941 gtttggcggg gtgcacgttg acgtccacct gatgcggatc gatctccaga tacagcacaa
    30001 atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc gcgtgattga
    30061 tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata ctgaatttct gccagtgcgg
    30121 gcgtggtgtg atttggatcg gccacccagc cgcgtagcgt gagatcgccg tgttgccatt
    30181 caatcgccag cgcttgttca agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    30241 tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta tgcgacaggt
    30301 tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca atgtggttaa
    30361 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacagatcca
    30421 gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg ttcatatcgc
    30481 gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    30541 ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg
    30601 cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc gccagcgcca
    30661 gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    30721 gttcgatatc aatatcgata cgtgtcgcac ctgcatcgag gctgttttcc accagttctt
    30781 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    30841 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    30901 tcaccgtact ggccgtttgt gccgttgccc cctgcggcgc agattgcatc ggatgcgcaa
    30961 ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    31021 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgatg
    31081 ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt
    31141 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgttggg
    31201 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    31261 ccagcacatc acccgcccca cccagcagct ccgactgttt ctcgtgctgc tccagccagc
    31321 tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg ccagtcgcac
    31381 tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg
    31441 ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgccct
    31501 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    31561 ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    31621 caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta
    31681 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    31741 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg
    31801 gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt aaagacgaca gtgtaattgc
    31861 tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg accaccagcc
    31921 gtagcgtttg tgcatcttta ggcgttccag agcgaatagc cttcacaaga ttattgccgc
    31981 tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc
    32041 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    32101 cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc accggcgtgc
    32161 acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc ccgccacctt
    32221 tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa accgcactca
    32281 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    32341 gaacaccagt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    32401 cgcgaatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    32461 aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    32521 taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta
    32581 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    32641 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    32701 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    32761 cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac acagcccgca
    32821 caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    32881 acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat gcctaaagcg
    32941 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    33001 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    33061 aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg cttatcggga
    33121 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    33181 actttttgca gtgctttttt cccccactct tgctggccca gaccgggacc aatcaccacc
    33241 acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg caccatcaat
    33301 tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac tcggactaaa
    33361 ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    33421 tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt
    33481 tttagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc
    33541 agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt
    33601 ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcact
    33661 gcgcctggcg tagcgccggt ttcagccagc aggccggaag ggatatcaac cgccgcaatc
    33721 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggcgcttgc
    33781 tgcaagccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg ccagacaata
    33841 ttcgaagcat ggatctcgcc tcccgcgttt aaccatgctt cgcgtgccag cgcggcctct
    33901 tccggcaacg gtttgtcgct ctcctgggcc agcagcgtga cctcaatgcc gaccgctgtg
    33961 gccagccgcg cgaccacgta gccatcgccg ccgttattac catgaccgca cagcaccagc
    34021 caatggcggg cgtcaggata cgccgaacga cacacctgga atgcggcttc gccagcgcga
    34081 agcatcagct catagagtgt aagccccagc gcatctgccg cctcacgttc tccgcggcgg
    34141 atatcgtcgg cgtgccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    34201 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtggggg
    34261 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga gtccgagccc
    34321 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcacgtcac
    34381 ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt gatcagcgtg
    34441 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa ccccaaactc
    34501 ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg caaccgactc
    34561 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    34621 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt
    34681 aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg cgaattgctg
    34741 gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa atgcgtggcc
    34801 tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga tgctcgccgc
    34861 tgtatctctt atctcaccat cgaactggaa ggggcgatcc cggaagagtt gcgaccgtta
    34921 ataggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    34981 tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc accggaactc
    35041 attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg atcggcgatt
    35101 cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg caatgcccct
    35161 tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc acttctcgat
    35221 gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg catagtcgaa
    35281 gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac
    35341 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagacgaata
    35401 aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta tctatcaata
    35461 ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta cagcctgagg
    35521 ctgtggataa ctctgtttac aagagtttgt cagagacaac gaaaaccagc aataacgcat
    35581 cgcatcgctg tggatatttt attgagagaa gaatttggag cgggaaacga g
//
LOCUS       DAZFVO020000027        35617 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFVO020000027 DAZFVO020000000
VERSION     DAZFVO020000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.027"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..556
                     /locus_tag="ACHKNB_RS06550"
                     /old_locus_tag="pgaptmp_001310"
     CDS             50..556
                     /locus_tag="ACHKNB_RS06550"
                     /old_locus_tag="pgaptmp_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4194864.1"
                     /translation="MILDQEWILASDLLGVGGLPSSLAGLHKRAKNEGWEKRSVTTPG
                     IRGRAFAYRLSDLPAHVVSIITGSAEIKHQPPQKNGGNSELLELIDTLSQEEQELVIY
                     TLRRKGVEKLLEFCNQENQELIALTGIRRLAALSLNNLSDQKVREIFEGDETKDHHFN
                     LTHKEAKA"
     gene            862..969
                     /locus_tag="ACHKNB_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /pseudo
     CDS             862..969
                     /locus_tag="ACHKNB_RS06555"
                     /old_locus_tag="pgaptmp_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1027..1926)
                     /gene="yfeX"
                     /locus_tag="ACHKNB_RS06560"
                     /old_locus_tag="pgaptmp_001312"
     CDS             complement(1027..1926)
                     /gene="yfeX"
                     /locus_tag="ACHKNB_RS06560"
                     /old_locus_tag="pgaptmp_001312"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HGU4194865.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(2022..2597)
                     /gene="yfeY"
                     /locus_tag="ACHKNB_RS06565"
                     /old_locus_tag="pgaptmp_001313"
     CDS             complement(2022..2597)
                     /gene="yfeY"
                     /locus_tag="ACHKNB_RS06565"
                     /old_locus_tag="pgaptmp_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HGU4194866.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(2658..3107)
                     /gene="yfeZ"
                     /locus_tag="ACHKNB_RS06570"
                     /old_locus_tag="pgaptmp_001314"
     CDS             complement(2658..3107)
                     /gene="yfeZ"
                     /locus_tag="ACHKNB_RS06570"
                     /old_locus_tag="pgaptmp_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HGU4194867.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(3094..3519)
                     /gene="ypeA"
                     /locus_tag="ACHKNB_RS06575"
                     /old_locus_tag="pgaptmp_001315"
     CDS             complement(3094..3519)
                     /gene="ypeA"
                     /locus_tag="ACHKNB_RS06575"
                     /old_locus_tag="pgaptmp_001315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGU4194868.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3733..4602
                     /gene="amiA"
                     /locus_tag="ACHKNB_RS06580"
                     /old_locus_tag="pgaptmp_001316"
     CDS             3733..4602
                     /gene="amiA"
                     /locus_tag="ACHKNB_RS06580"
                     /old_locus_tag="pgaptmp_001316"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HGU4194869.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            4606..5505
                     /gene="hemF"
                     /locus_tag="ACHKNB_RS06585"
                     /old_locus_tag="pgaptmp_001317"
     CDS             4606..5505
                     /gene="hemF"
                     /locus_tag="ACHKNB_RS06585"
                     /old_locus_tag="pgaptmp_001317"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HGU4194870.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(5511..6563)
                     /gene="eutR"
                     /locus_tag="ACHKNB_RS06590"
                     /old_locus_tag="pgaptmp_001318"
     CDS             complement(5511..6563)
                     /gene="eutR"
                     /locus_tag="ACHKNB_RS06590"
                     /old_locus_tag="pgaptmp_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HGU4194871.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(6609..7109)
                     /gene="eutK"
                     /locus_tag="ACHKNB_RS06595"
                     /old_locus_tag="pgaptmp_001319"
     CDS             complement(6609..7109)
                     /gene="eutK"
                     /locus_tag="ACHKNB_RS06595"
                     /old_locus_tag="pgaptmp_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HGU4194872.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKENGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(7122..7781)
                     /gene="eutL"
                     /locus_tag="ACHKNB_RS06600"
                     /old_locus_tag="pgaptmp_001320"
     CDS             complement(7122..7781)
                     /gene="eutL"
                     /locus_tag="ACHKNB_RS06600"
                     /old_locus_tag="pgaptmp_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HGU4194873.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(7791..8678)
                     /gene="eutC"
                     /locus_tag="ACHKNB_RS06605"
                     /old_locus_tag="pgaptmp_001321"
     CDS             complement(7791..8678)
                     /gene="eutC"
                     /locus_tag="ACHKNB_RS06605"
                     /old_locus_tag="pgaptmp_001321"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HGU4194874.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(8699..10060)
                     /gene="eutB"
                     /locus_tag="ACHKNB_RS06610"
                     /old_locus_tag="pgaptmp_001322"
     CDS             complement(8699..10060)
                     /gene="eutB"
                     /locus_tag="ACHKNB_RS06610"
                     /old_locus_tag="pgaptmp_001322"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HGU4194875.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(10072..11475)
                     /gene="eutA"
                     /locus_tag="ACHKNB_RS06615"
                     /old_locus_tag="pgaptmp_001323"
     CDS             complement(10072..11475)
                     /gene="eutA"
                     /locus_tag="ACHKNB_RS06615"
                     /old_locus_tag="pgaptmp_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HGU4194876.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(11472..12698)
                     /gene="eutH"
                     /locus_tag="ACHKNB_RS06620"
                     /old_locus_tag="pgaptmp_001324"
     CDS             complement(11472..12698)
                     /gene="eutH"
                     /locus_tag="ACHKNB_RS06620"
                     /old_locus_tag="pgaptmp_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HGU4194877.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(12815..14002)
                     /gene="eutG"
                     /locus_tag="ACHKNB_RS06625"
                     /old_locus_tag="pgaptmp_001325"
     CDS             complement(12815..14002)
                     /gene="eutG"
                     /locus_tag="ACHKNB_RS06625"
                     /old_locus_tag="pgaptmp_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HGU4194878.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(13992..14828)
                     /gene="eutJ"
                     /locus_tag="ACHKNB_RS06630"
                     /old_locus_tag="pgaptmp_001326"
     CDS             complement(13992..14828)
                     /gene="eutJ"
                     /locus_tag="ACHKNB_RS06630"
                     /old_locus_tag="pgaptmp_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HGU4194879.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(14839..16242)
                     /gene="eutE"
                     /locus_tag="ACHKNB_RS06635"
                     /old_locus_tag="pgaptmp_001327"
     CDS             complement(14839..16242)
                     /gene="eutE"
                     /locus_tag="ACHKNB_RS06635"
                     /old_locus_tag="pgaptmp_001327"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HGU4194880.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(16254..16541)
                     /gene="eutN"
                     /locus_tag="ACHKNB_RS06640"
                     /old_locus_tag="pgaptmp_001328"
     CDS             complement(16254..16541)
                     /gene="eutN"
                     /locus_tag="ACHKNB_RS06640"
                     /old_locus_tag="pgaptmp_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HGU4194881.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(16648..16941)
                     /gene="eutM"
                     /locus_tag="ACHKNB_RS06645"
                     /old_locus_tag="pgaptmp_001329"
     CDS             complement(16648..16941)
                     /gene="eutM"
                     /locus_tag="ACHKNB_RS06645"
                     /old_locus_tag="pgaptmp_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HGU4194882.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(16980..17996)
                     /gene="pta"
                     /locus_tag="ACHKNB_RS06650"
                     /old_locus_tag="pgaptmp_001330"
     CDS             complement(16980..17996)
                     /gene="pta"
                     /locus_tag="ACHKNB_RS06650"
                     /old_locus_tag="pgaptmp_001330"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGU4194883.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(17993..18796)
                     /gene="eutT"
                     /locus_tag="ACHKNB_RS06655"
                     /old_locus_tag="pgaptmp_001331"
     CDS             complement(17993..18796)
                     /gene="eutT"
                     /locus_tag="ACHKNB_RS06655"
                     /old_locus_tag="pgaptmp_001331"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HGU4194884.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(18793..19494)
                     /gene="eutQ"
                     /locus_tag="ACHKNB_RS06660"
                     /old_locus_tag="pgaptmp_001332"
     CDS             complement(18793..19494)
                     /gene="eutQ"
                     /locus_tag="ACHKNB_RS06660"
                     /old_locus_tag="pgaptmp_001332"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HGU4194885.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(19469..19948)
                     /gene="eutP"
                     /locus_tag="ACHKNB_RS06665"
                     /old_locus_tag="pgaptmp_001333"
     CDS             complement(19469..19948)
                     /gene="eutP"
                     /locus_tag="ACHKNB_RS06665"
                     /old_locus_tag="pgaptmp_001333"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HGU4194886.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(19961..20296)
                     /gene="eutS"
                     /locus_tag="ACHKNB_RS06670"
                     /old_locus_tag="pgaptmp_001334"
     CDS             complement(19961..20296)
                     /gene="eutS"
                     /locus_tag="ACHKNB_RS06670"
                     /old_locus_tag="pgaptmp_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HGU4194887.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(20588..22867)
                     /gene="maeB"
                     /locus_tag="ACHKNB_RS06675"
                     /old_locus_tag="pgaptmp_001335"
     CDS             complement(20588..22867)
                     /gene="maeB"
                     /locus_tag="ACHKNB_RS06675"
                     /old_locus_tag="pgaptmp_001335"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HGU4194888.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            23156..24106
                     /gene="tal"
                     /locus_tag="ACHKNB_RS06680"
                     /old_locus_tag="pgaptmp_001336"
     CDS             23156..24106
                     /gene="tal"
                     /locus_tag="ACHKNB_RS06680"
                     /old_locus_tag="pgaptmp_001336"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4194889.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            24126..26129
                     /gene="tkt"
                     /locus_tag="ACHKNB_RS06685"
                     /old_locus_tag="pgaptmp_001337"
     CDS             24126..26129
                     /gene="tkt"
                     /locus_tag="ACHKNB_RS06685"
                     /old_locus_tag="pgaptmp_001337"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4194890.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(26225..27268)
                     /gene="ypfG"
                     /locus_tag="ACHKNB_RS06690"
                     /old_locus_tag="pgaptmp_001338"
     CDS             complement(26225..27268)
                     /gene="ypfG"
                     /locus_tag="ACHKNB_RS06690"
                     /old_locus_tag="pgaptmp_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HGU4194891.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(27394..27969)
                     /gene="nudK"
                     /locus_tag="ACHKNB_RS06695"
                     /old_locus_tag="pgaptmp_001339"
     CDS             complement(27394..27969)
                     /gene="nudK"
                     /locus_tag="ACHKNB_RS06695"
                     /old_locus_tag="pgaptmp_001339"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HGU4194892.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(28037..30016)
                     /gene="aegA"
                     /locus_tag="ACHKNB_RS06700"
                     /old_locus_tag="pgaptmp_001340"
     CDS             complement(28037..30016)
                     /gene="aegA"
                     /locus_tag="ACHKNB_RS06700"
                     /old_locus_tag="pgaptmp_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HGU4194893.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            30222..31922
                     /gene="narQ"
                     /locus_tag="ACHKNB_RS06705"
                     /old_locus_tag="pgaptmp_001341"
     CDS             30222..31922
                     /gene="narQ"
                     /locus_tag="ACHKNB_RS06705"
                     /old_locus_tag="pgaptmp_001341"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HGU4194894.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            32086..35199
                     /gene="acrD"
                     /locus_tag="ACHKNB_RS06710"
                     /old_locus_tag="pgaptmp_001342"
     CDS             32086..35199
                     /gene="acrD"
                     /locus_tag="ACHKNB_RS06710"
                     /old_locus_tag="pgaptmp_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HGU4194895.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPKLTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(35298..35357)
                     /gene="ypfM"
                     /locus_tag="ACHKNB_RS06715"
                     /old_locus_tag="pgaptmp_001343"
     CDS             complement(35298..35357)
                     /gene="ypfM"
                     /locus_tag="ACHKNB_RS06715"
                     /old_locus_tag="pgaptmp_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HGU4194896.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         8233 a         9928 c         9194 g         8262 t
ORIGIN      
        1 aaaccacata aaagatatga acaaaagtta atatcaaatg gagtttgata tgatccttga
       61 ccaagaatgg atccttgcca gtgacttgtt gggggttgga ggtcttcctt catctctggc
      121 tgggcttcat aaacgagcaa aaaatgaagg atgggaaaaa cgttcggtaa ctacacctgg
      181 tatcagaggg agagcatttg cctaccgttt aagcgactta cctgcacatg ttgtttccat
      241 cattacaggt agtgcagaaa taaaacacca gccccctcaa aagaacggtg gcaatagcga
      301 gttgctcgaa ctcatcgaca ctttgagcca agaagagcaa gaattagtta tttacacctt
      361 gagaaggaaa ggggtagaaa aattgctgga attctgcaat caagagaatc aagagttgat
      421 cgctttaaca ggtattcgac gcctggcagc attgagcctt aataatttat cagatcaaaa
      481 ggttagagag attttcgagg gagatgaaac caaggatcat catttcaatt taacccataa
      541 agaagccaaa gcgtaacccc atgaagccca ggccatgcac ttagcgagca ctttcaactt
      601 gatttgtgac cagatcgata ttgatataaa ctttttagca taaaagacat tttaagtact
      661 tttaaaaacg tccataagca gtatgtgcag tatcaaattt gttgccgata ccttaatttt
      721 ataccggatt ttatttaatc agtatcaaaa ctaaacaacc tccagaatca tcgatcacct
      781 gttttatttc agtatcttac aggtgatttc acctcctttc actaaaaccc ttgtcggtat
      841 caaatgattt acctggttac agttgccgat ccgcaaaaga atcccaatat gttcgaaagc
      901 ggtcagctgc cgattgcaac gtatggttgg gtagttgatc aggtgtatgc ggcgttaaag
      961 caaaagtaat aaaaaggcca gtcagtaatg ttactgcctg gcctttaatc aatgaatcag
     1021 aaacgcttac agcgccatca acttgtccag cgacggtgcg aaataatagc cgccggttac
     1081 cggtttggtg aaacgcaaca tcgcatcacg cttaccatcg gtatcgccaa acatgctcag
     1141 cagttgctgc tcaatgttat gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt
     1201 gccactggca gtgccgtacg gcaggctctg gcgaacaatc ttcagccctt tgccatcttc
     1261 tttcagatca acgcgggtga ggtgagaggt ttccggacgt tcgtcgccgt cgatctcttc
     1321 gttggcctct ttggtgcgcc cgatcatcat ctcctgatcg tgaacgctca tccggttgag
     1381 ctgcttcagg ttgtgttccc aacgctggac aaacacatag ctgccgcccg catccacgcc
     1441 gtctttgata accgccactt cgcgacgcgt ctcttcaccc gccgggtttt ccgtaccgtc
     1501 aacaaagccg ctcaggtcac gctcttcaac ccaacggaag ccgtggatct cttctttcac
     1561 ttcaatgcag tcaccaaagg cttccatcgc cgcctgggcg acagagaagt ttacgtcgtg
     1621 acgcagagaa agaatgtgga tcaacacatc gaactgggtg gtcggcgcaa ggcctttacc
     1681 gtagcccgga aaatctttca gctcttctgc cccaacgccg ccgctcagag cgcgccaggt
     1741 gttgttacca aaggcaacca ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt
     1801 tgccagtttg tcggcaaatg ttttactggc cgcacgcagg gcgtcaactt cccctttcac
     1861 gttggcttcg atccaaatcg ccgcgcggca atgttctggc aaaatgccac tctgaacctg
     1921 agacattgtt cctcctgaaa ataataatgc cacgcaatcc gtggcattgt tgagcgctat
     1981 tttacgtgtt ttttcgcggc ggcggtttgt tcagacgcaa attaacgccg ccagataatt
     2041 ttactgactt tccagttttt cagggtatcg tccgaaggca ttaacccttc aggaccactc
     2101 cattccccgc tgaactggta gctaatatgt tgactgcctt cggctttaca ttcgacggca
     2161 cgattatcat caccatcagc tttttggcaa ttgccaaatg ctttgctgta aaggtcgcta
     2221 aacggtgtac cgattttaac accggtgtca gcaggaatat cgctatccag cacgtcaatg
     2281 cgactgatcg tgccttgatc gccgttaatc accatcgcca cgttgtcgcc cttcatcact
     2341 tcaaaaaagc gcaccacgtt gccgttcgcg gttttcattc cgctgcgcag gcgataatcg
     2401 ccatcaagcg catcggcaat ggcttgttct tgcagtggtg tggacgccgt taattcacca
     2461 acgccctgct cgctcacttt ggtggacgac ccaaaccagt tccacgggtt agcggccgac
     2521 cagttaactg acgacatcgt ggaacagccg gtcagcatca acggcatagc gcataacatt
     2581 aaacgcagcg atttcatgtc acttcctttg gttattcaat aacgttgctt ggagtgcaaa
     2641 ttcaccaaaa agtgccgtta ttctttttct tcattaaaac aagcgcgtaa acgtcgattg
     2701 gtcagcagcc agattaacgc cacaatatcc gccactacca gcgccagacc gataccgcta
     2761 acggattcac cgttcagcca cagccacggt tgccagcaaa gtaaaaccac ctgcgccaac
     2821 agcaacagaa aatagagcac atgccaagtg cgaggaaatg tagcccgccg accgctcaac
     2881 aaaaacgcca gcaccgccgg aatgcctgga atcagcccca gccagaaatt atcgtgatcg
     2941 ggataaaaca gatttagcag cgcagtaccc tgctcgcgcg acgcaccggc aatgacaaac
     3001 agcacccagg ttcgcgcctg aagcaatagc acaagccaga agagcaaggg taaacgcagg
     3061 cgtccgtgcg catcataatg gacaggatgg aactcagtac tcttcatctt caatcaaacg
     3121 cttacccaga ctcagcacgt cggcgtgttc atatcccagg cgttcataca ttccgagcac
     3181 catgtcgtta tcttccggca cattgatctg aattttcggg cagccacgag caatcagctt
     3241 tttctccagc cgattaagca acgcattggc aatcccacgc ccacgaaact ctggatgcac
     3301 gccaagataa taagcagacc cgcgatgccc gtcataaccg cccatcaccg ttccgaccac
     3361 ttcaccgttt acctcagcga ccaaaaacaa actgacgtca tggttcatct tacgctcgat
     3421 gtccatttcc ggatcgttcc acggacgcag caagtcgcaa cgctcccaaa gggtgatgac
     3481 ctcttcgaaa tcttcctggc gaaatacgcg tatctccatg gtattcgtta cctttttgcg
     3541 ggttaaaagg ctgattatgg cgtgaacggt cgaattagcc aatatctgac gaaaatcggt
     3601 tgaaaaagtg gcataatggg gagttgtcaa ctattgaaat gaaaagtaaa acaattctca
     3661 acatcaaacc gtcgtaacgg attacgcgat acgatataac atctggaact ttattattac
     3721 aactcaggcc gtatgagcac ttttaaacca ctaaaaacac tcacttcgcg ccgccaggtg
     3781 ctgaaagccg gtttggctgc cctgacgttg tcaggaatgt cgcaagccat cgccaaagac
     3841 gaacctttaa aaaccagcaa cggacacagc aagccgaaag ccaaaaaatc tggcggcaaa
     3901 cgtgtcgttg ttctcgatcc aggccacggc ggaattgata ccggagcgat cggacgcaac
     3961 ggttcgaaag aaaaacatgt ggtgctggcg attgctaaaa acgtccgttc cattttgcgt
     4021 aatcatggga ttgatgcgcg tttaacgcgt tctggcgata cgtttatccc actttacgat
     4081 cgcgttgaaa tcgcccataa acatggcgca gatctgttta tgtcaattca tgccgatggc
     4141 tttaccaacc cgaaagctgc cggtgcttcg gtatttgccc tctctaaccg tggggcaagt
     4201 agcgcaatgg cgaaatacct gtctgaacgc gaaaaccgcg ccgatgaagt tgccggtaaa
     4261 aaggcgactg acaaggatca cctattgcag caagtgctgt ttgatctggt gcaaaccgat
     4321 actattaaaa acagcctgac gctcggctcg catattctga aaaagattaa gccggtgcat
     4381 aaactgcaca gccgcaacac cgaacaagcc gcgtttgtgg tgttgaaatc gccgtcggtt
     4441 ccttcggtgc tggtggaaac ctcgtttatc accaacccgg aagaagagcg gctgttaggc
     4501 acggcggcgt ttcgtcagaa aatcgccaca gcgattgctg aaggcgtgat cagttatttc
     4561 cactggttcg acaaccagaa agcacattcg aaaaagcgat aagttatgaa acccgacgca
     4621 caccaggtta aacagtttct gctcaacctt caggatacga tttgtcagca gctgaccgcc
     4681 gtcgatggcg cagaatttgt cgaagatagt tggcagcgcg aagctggcgg cggcgggcgt
     4741 agtcgggtgt tgcgtaatgg tggtgttttc gaacaggcag gcgtcaactt ttctcacgtc
     4801 cacggcgagg cgatgccagc ttccgccacc gctcatcgcc cggaacttgc cgggcgcagt
     4861 ttcgaggcga tgggcgtttc actggtagtg catccgcata acccgtatgt tcccaccagc
     4921 cacgcgaatg tgcgtttttt tattgccgaa aaaccgggtg cagagcccgt ctggtggttt
     4981 ggcggcggct tcgatttaac ccctttctat ggttttgaag aagacgccat tcactggcac
     5041 cgcaccgccc gtgacctgtg cctgccattt ggtgaagacg tttatccccg ttacaaaaag
     5101 tggtgcgacg attacttcta cctcaaacat cgcaacgaac agcgcggtat tggcgggctg
     5161 ttctttgatg atctgaacac gccagatttc gaccactgtt ttgcctttat gcaggcggta
     5221 ggcaaaggct acaccgacgc ttatttacca attgtagagc gacgtaaagc gatggcctac
     5281 ggcgagcgcg agcgcaattt tcagctctac cgtcgcggtc gttatgtcga gttcaatctg
     5341 gtctgggatc gcggcactct gtttggcctg caaactggcg ggcgcaccga gtcgatcctg
     5401 atgtcaatgc cgccactggt acgctgggag tatgattatc agccaaaaga tggcagccca
     5461 gaagcggcgt taagtgagtt tattaaggtc agggattggg tgtaactccc tcacccccat
     5521 tcccgcatcc gctgatgcaa cgtcaacgac ggcttctcgg caaacagctg ctggtaatcc
     5581 gtggcaaatt gccccagatg ccagaatccc cactgcatgg cggcgtcttt taccgttgtg
     5641 ctttgcgacc acgggcttat cagttcgcgg cgtacggcgt tcaggcgaat gcgtttcagc
     5701 cacgcgtttg ggccaatgcc taaaatagcg tgaaacgcgt tttgtagcgt gcggcgactg
     5761 acatgcagtt gattacacaa gtccagcacc gtcaccggct cagacatatt ttccagcaca
     5821 tattcacggg cgcgggaaag taatctacgg taactctgat gactgatgct ttccgccgtc
     5881 accattggtt gcgcgtcttc cagcatcgcc cccatcgcca ttagcaaatt atcccccagc
     5941 acttttcgca ctgcaggctg atggagattt tccgggttct cgcaaaacgt cgccagcgcc
     6001 tgttggacaa agccccacag cgcggcttta tgctgctctt tcacttccag cgccgactgg
     6061 ctacgcagca tatgtaatac ccgatccggg ttatgcaaaa agttagcctg ccgggtgatg
     6121 acatcttcag aaagcaccac gcccaggatc gtgtaatcat ccggcgtgct cagttcaaat
     6181 tcagtgccac cagggcgggt ggcgatttcc gcgcttccca gacattgcga accgataaat
     6241 ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccaggcac
     6301 gactgacgca gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt
     6361 tccgtaaatt caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg
     6421 gtaatcgtta aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact
     6481 tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa
     6541 ttggctgtac gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct
     6601 ccgacaggtt aatggggctt gagacgataa cgactactgc gtttacgtaa cgtcccggca
     6661 gaaaagagct gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcagcc
     6721 acttctcctg ccgtcattcc ctgacgtact gatgttaaca gcgccaacaa ctcgtcagca
     6781 gattccgcga cgataactgg cgcgtcgggt tcccttacgg gttgcttcgg ctggcggtta
     6841 aagccagtaa ccagccactg ggtgtcatcg tctggccgac cgttctcctt gcggctgatg
     6901 acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc
     6961 agatcgcctt ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga
     7021 cgaacgttag ctgctttgag catggcatcc gctgcatcta tcgcggcgac catgccgtcc
     7081 acttccagca atcccagtgc attgatcatc ggcaacctcc gttacgcacg ctggattgga
     7141 ttacgcgcga tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgcggcc
     7201 tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacataggtc
     7261 accagctgta cgtcggcaga tttcaacgct gcatcaatgc cataggtcgc ttccagcggc
     7321 ggtgccacca gatacgccat cggatcgcca agcgtgatcc cggcggttga tgagagataa
     7381 gaaccggtac gcgaaaccac atgcgccagg aaggcggtgt tttctgcgtc attggcccac
     7441 tggaatgctg caccattttc aatatgtgca accatcgcat ccagaccggc acgcacttcc
     7501 gccgggtttg gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt
     7561 gcagcgcccg catacagcga gcgaccatat accacttcca ccatcgcctg cttggtcgct
     7621 tcatcagcgg caatgtaggt gacgtcatcg gaatcagcag aaatcagccc gaggctacga
     7681 atatgcggcg gcaattttag ttcacgcgca aattcagcgt taacggaggc aatcacccgc
     7741 atggcggtta ccgacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc
     7801 atgttgatgc cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggct
     7861 gcggcttcaa ccggcggcgt gccgccctgg tgaatgttag agatacaggt gcgatcggcc
     7921 tcaacggtgg tcgccatacg cggcgagtaa acggcgtagc aggagaggct ttctgactgc
     7981 cctaaccccg gacgttcccc caccagcagg atcaccactt tcgcgccgag gatctcaccg
     8041 atctgatctt caatcttcac gcgaccataa cgaacaaaga atggcgtgcc gactttcagc
     8101 ccggcctgtt tcaggcccgc catcagcggc ggcaggatct cttcgtagtt cacggtgatg
     8161 gcatcagttg acaggccatc agaaataacg acctgtacgt ccggattagc aacacactgt
     8221 gctttcagcg cttcaacagc ttctgcacac aggcggcggc ccatatccgg gcgagtcagg
     8281 tagagatttt tgtcgctaat ttcagagcgt acttccagca agccctgcgc tttcacccac
     8341 tcttccggca cttctttcag tacggtatct ttcgaacgag agtgatcggc caggaaacgc
     8401 agcagcgcct gggtacgtgg acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg
     8461 ctgcggcgca gttctgttaa tacgtctgcg cgatgcgggt tttcaacacc aatccacgct
     8521 tttgcttcag cggaacctaa atccagcgca cagctttccg aggtcaccgg tgccgcacag
     8581 ttggtggtgg cgcactttgc ttctgacggc gcgggggccg tttgtcccat tgacgccatc
     8641 acgctgcgta caatttcttc aatctgtttt tgatccatga tgtgttatct ccgcgtcatc
     8701 agaagaacag tgacggatcg cccgcccgtt tggtcaggcg accgtttgcc ataatgccca
     8761 tgctttccag ccagcgttca aactctggtg acgggcgcag gttgagtaac tgacgcacag
     8821 tggcggtatc gtggaatgcg gtggtctggt agttgagcat gatgtcatca cccagcggca
     8881 tccccatgat gtagttgcag cctgcggtgg cgagcaggat catcaggttt tcgttgaggt
     8941 tctggtcagc gtcagcgtgg ttggtgtagc agcagtcaca gcccatagag atgccgctca
     9001 gcttgcccat aaagtgatct tctaagccag cacggataat ctggcggtcg ttgtagagat
     9061 actccggccc aataaagccg accacggtgt tgacgataaa cggatcgtaa tgacgcgcca
     9121 gcccatagtt acgtgcttcc atcgtcacct ggtcagcgcc gaagttagcg ccagcggata
     9181 gcgcagagcc ttgtccggtt tcgaagtaga ggcagttttc cccggcgata cggttgaact
     9241 ctgcacccac tgcgcgcgct tcgtcgagca tcgccagttc cacgccaaac tctttcagcc
     9301 ctttttcgct gccacagata ctctggaaaa tcagtccgcc aggcgcgccg cgacgaatcg
     9361 cttcgatctg ggtggtgacg tgcgccagta cgcagccctg agttgggatg ttgaatttgt
     9421 cgatcacgcc gtaaatggtg tccaacacgc ggcttaagtt ttccacgtcg tcagtcaccg
     9481 ggttaacgcc gatcaccgca tcgcccaccc cgaaggaaag cccttcgtag atttgcgcgg
     9541 cgatactttg cacgtcgtca cgggtgtcat ttggctgcaa acgggcgctg aaggtgcccg
     9601 gaataccaat ggtggtattg gcctttttga ttaccggcat tttcttcgcg ccgtagatca
     9661 ggtccgcgtt ggagcaaatc ttcgctaccg ccgcgaccac ttccgaggtc agccctttgc
     9721 gggtaaaggc aatgtcgtcc acgctggttt catcgctcag cacatactca cgcagttcgc
     9781 tgatgctcca gtttttaatc tggttgtagg ccgtttcgtt aacatcgtcc tgaatcagcc
     9841 gcgtcacgca gtcatcttca taggcaatca ccggattatt gcggatgtcc gctacggtca
     9901 tttccgacaa cacctgcttt gccgccacgc gctcctgtga gcttgccgct gcaacgcccg
     9961 ccagcacatc ccccgaacgc agttcgttgg ctttagccag cacctctttt acatccttaa
    10021 actgatatac attgccgaac aatgtggtct ttagtttcat aagtcgttcc ctcaggaagg
    10081 aaatgcgagt gatttcaccg tcaccggcac aaccgatccg ccaaaaagag gcgtaccaat
    10141 gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg actgccaacg ggagttgttg
    10201 tagctgtggg cgcaacaaca tgcccagagc tttaccaaag tcctgcccgg ccaccaccag
    10261 caggggatgc ggattcggaa aacgcgcgac gaaatcgacc agcgcgttga tgaccgtcag
    10321 taccgcggcg taacgcacag gcagcgaggc gggaagcgcc agcacgtacg cgtcagtttt
    10381 gggatcaaga tccagctgaa gcagcgcctg ttgccaggca ctcaccagat ccgtttcatc
    10441 aatcgggatc gccaccggca aattgcgcag cggcagttgt acgccctcca gccagattgt
    10501 gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc accgtggcgc gtacggtttg
    10561 cgccggaaac tgcacattca tctcacgcag gcgcggatgg tcatgcagcg ccgttgccag
    10621 aagtgggcca atatcggcaa aacagaacgg gtcggcgggc tggtggcgat aacattcgcc
    10681 cacgcctcca gaaagcgtaa tgatttcggg cgtaacacct gcgggcagca aaccggtttg
    10741 catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc acttcgacaa tcagcgccgc
    10801 catccgccgg gtaacctgca ccagctgcgc gccggtcagc gaacgggcgt cagtgcctgc
    10861 accgaagcac tcatccacaa tcatctgccc cggtttatga gcgtaaacca cgcgcccctg
    10921 gctgtcggtt tccagcaggc gaccaccgac gttgagacag gcagtgccgc tgatttttcc
    10981 ggcatcgaac agggcgtagt tcgcggtgcc accgccgatg tcgatattca gtacccgaca
    11041 cagccgttgt tcagaaaggg tttgcgcccc agctccgtga ccggcgatca cggattcgag
    11101 gtgcggcccg gcgctggcaa cgacaaaatc ccccagcgat tgagagagcg ccatcaccgc
    11161 cgggcgagca ttgcgggttt tcgcgctttc accggtgatg atgatggcac cagaatcaac
    11221 gctttccggc gcaatacccg cagcctgata ttgctcgagt attaaggttt tcagttccgc
    11281 ttcttttaaa ccgccctgtt tatcgacagg ggtaaagaac accggacttt gccagctaat
    11341 ttcgcgttta atgaattcgt agcgcggcac ctgcgacacc gccgcacggt taaccagctc
    11401 caggtgggag aaaatcacct gggtggtggt ggtgccgata tcgataccga cgctcaatag
    11461 ctggcgagtg ttcacgattg tgcctccgct tcggttttag tcgcggtcgc gtcttctttt
    11521 ggcaccagca tcatcgccac gccaatcgcc gttacgccgc cgatcaactt gccgacaatc
    11581 atcgggaaga tcatggcgtt catgttggca gcggcgaagc ctaagtggtc gcccagggcg
    11641 aaagcagcgg aaacggcgaa ggcgcagttg atgactttgc cgcgggtatc catctgcttc
    11701 atcatgccga acatcgggat gttgttggca agcgttgcca ccatgccggc tgccgcgatg
    11761 ttgttcatat tcagcacttt accgacgctc atcagcggtt tttcaaacca gcgagtcagc
    11821 agcagcacca ttggatacgc ccctaacagc acgcaggaga tagaaccgat aacttcaatg
    11881 gcgcgcatca cttcaccggg tttatcgcca ggggccataa agataggatc cagtccgggg
    11941 atcagttccc agccaaggag gaatttcact accgcagcgg caagaccgag ggtgatcaat
    12001 gcaacgagga atttggcgaa gatctggaag ccgttgatca ttttttccgg gatgaatttc
    12061 agccccagcg ccaccagaat cgcgacaatg atcaccggga tcatgttcat caggatcagg
    12121 gcgaaagtga attccaccgg ctgaccgttg atctgcacac cggagtacat agcaaccaga
    12181 ccaccagcaa tacagccaat cggaatggtc acaatgcccg ccagcacgcc gagcgccaga
    12241 taacgacggt cagaaggttc gataatgccg agcgccaccg gaatggaaaa cacaatcgtt
    12301 ggccccatca tcgacccgag aattaaccca gagtatagcc acgctgctac gtcgccgccc
    12361 gccagctctt tggcgaggaa gaagccgccc atatcgcacg ccagcagtgt tccggcgaac
    12421 atcgatgggt tagcgccgag catttcgtaa accggaataa ttaccggccc gagtacgtga
    12481 gccagtaccg gtgccagcgc ggtcataccg accatcgcca ggcccagtgc gcccattgcc
    12541 ataaagcctt cttcgaactg accgcctgat ccttcgatac ttttaccgaa cttaccgagg
    12601 aaacgagcag aaccgccgaa ctgcgacagg atcctgtcta cggcagctat cagcataaag
    12661 aacatcatga tgtacatgat gatttcgtta attcccatcg cctttactcc ctgttagttg
    12721 ttatttattg gcggatgcgg cgtaaacgcc ttatccgccc tacatgtgca atcccgaagg
    12781 ccggatcaga cacggcaagc gtcgcatccg gcatttattg cgccgctgcg tacaggccga
    12841 caatctgctc caggctggcg gtacgcgggt tgctgcgcag acaaatatct tcctgcgcgg
    12901 cctgcgccca tgcaccgtaa tgcgcagatg tcgcgccaac atcgcccagt cgtttaccaa
    12961 tcccaacttc cgcaatcagc tcacttaccg cgttaatagc gtcacgatcg tcggattttt
    13021 tagttcgcag tgcccgacca atctgactaa accgttcgcg acaaaccatc cggttaaatt
    13081 ccatcaccgt tggcagcaac atggcgttcg ccagaccgtg tggaatatgc agcgccgccc
    13141 ctggctgatg cgccatcgcg tggcacaacc caagccccgc actggaaaac gccattcccg
    13201 ccatacatga agccagtaac atgctctcgc gcgcggcaag gtcatgaccg tagcccaccg
    13261 ctttcggcag cgatttgcca atcatcgcaa tggcaccaat cgccagactg tcggtaaacg
    13321 gtgtggcgtt cagcgcgctg tacgcttcaa tggcatgggt taacgcatca atgccagtca
    13381 tcgccgtgac atgcgacggc acaccttcgg tcagcgcggc gtcgaggatc gccacatccg
    13441 gcatcagcga ggcatgggct aacacctgct tgcgcccgct caccgtgtcg ataatcaccg
    13501 ttacgttggt ggtttcagag ccggttccgg cggtagttgg aatggcaatc aacggcaggc
    13561 gcggttgcag aacgctggtt tctgacattt ccgccagcgt gctatccggg ttcgtcacca
    13621 gcaacgccac ggctttcgcc gcatccagca ccgagccgcc gccaaatgcg atcaccccat
    13681 cacagcctga ctcacgcaac tgcgccacgg ctgcacacac gtcggtgatg cacggttcgc
    13741 ccaccggaca tggccagagc gtcatggcga tgcctttaac agccaggctg cgcgtcagcc
    13801 cggcggtcat ccctgcctga tgcaaaaagc tgtctgccat cacgaacaga tgtttcagcc
    13861 cacgcgtttg cgcttgctgt ccgcaactgc tcaccgagcc cggaccgcaa agcgtcaccg
    13921 gtggaacgct aaatgttttt acccgttgca gattcagggt atcgaacgcc tgaaagagcg
    13981 cggtctgcaa ttcattttgc atatagcccc tccgctttct ctcgcccact actggcgatc
    14041 gccagcgggg tcataaacaa actgtgctgc ggtaaatgca cttgtaacgc cgggaattgt
    14101 ttgcgaaaca gttccgccac gcccggttgc atacaggagc cgcccgccag ccataaatca
    14161 gtaatgccct gcccttcaat atgccgggcg acaatgtccg ccattttttc gtacaccggt
    14221 ttcaccgcag gccaaatctc ttcaccgtga ccgcgcttgt actgctccgc ctcttccagc
    14281 gaaatacggc gatttccggc gagagtcaga gagatgtgat gtccgccggt ggcttcatcc
    14341 gccgagtacg tcactttgcc ctttttcacg atggcaatgc cggtagtgcc gccgccgata
    14401 tccactacac cggcgttgtc cagttgcagc agatccgcaa ctgcggttgg ctcatccagc
    14461 acatggctca cttccagtcc agcagactcc agcacgttga tggaaatacg cgggtccgta
    14521 cctggaggaa atgaagtcgc cgcatggcta aaacggcgac cgaattgctg ctcgagcgtg
    14581 tcgagatggc gacgaacaat ggtgacagcg ccgaagaaat cccagacgat gccgtcgcgg
    14641 accacatcgg cccagtcgag gcataccgcc accggctgac cgtcgcggtc gacaaccatc
    14701 gacaccacat cgcaggtgcc cagatccacc cccagccaca gcggagattc cgtcgcggcg
    14761 ggcgtctggt tacacagcgt tgccgccgtt tgcagacgtg gggtgagcca ttgttcgtcg
    14821 tgcgccatcg gttactcctt aaacaatgcg aaacgcatcg actaatacac agcgacgcag
    14881 acggacaaac gtccgcgcgc tggttacccc ttcaccggtt ggcgtggtga tggtcatggt
    14941 ggtccagcct tccccgccca gccccagccc ggcaatgcac ggtccgttct taacgaaaat
    15001 gctggtatca atagcattcg ccatctggtt catgttttcg atgttgcgcg agtgcattgc
    15061 cgccgtgtgg tggcaaccgc cttccagttt caccgccagc gcaatggcat ccgccacgtt
    15121 ggcgacgcgc acgacgggca gcaccggcat catcagttcg gtcacggcaa acggatgttc
    15181 tgcagtggtt tccacaaaca gcaaacgcgt ttcttgcggc acgttcaggc cgattgccgc
    15241 cgcgattttc gccgcatcgc gaccaaccca gtcacggctg acggtgcctt ttccgcgctc
    15301 gtcgatattt ttcagcaaca ccggttgcag ctgctgcgcc tgttctgcgg tcagtttcac
    15361 cgcgtgctgg ccttccatca gacgcatcag ttcatcggct acgctatcaa caacaatcag
    15421 taccttttcg tcggcacaaa tgatgttgtt atcgaaagaa gcgcctttga cgatggactg
    15481 agcggcacgg gcgaggtcgg cggtttcatc caccactacc ggcgggttgc cagcgcctgc
    15541 ggcaatcaga cgtttattgg tgtgtttacg cgccgcttct actaccgctt cgccgccggt
    15601 caccaccaga aggccgatgc ccggaaactt gaacaaacgc tgcgcggttt cgatatccgg
    15661 atttgccaca gtaaccagta agttttccgg cccgcctgcg gcaacaatcg cctggttgag
    15721 cagcgtgatc gcccgctggg agactttttt cgccgccgga tgcggggcaa aaatgacgct
    15781 gttgcccgcg gcaatcaggc tgatggcgtt attaattacg gttgccgccg ggttagtgga
    15841 cggagttacc gaagccacca cgccccaggg cgcgttttca attaaagtca ggccgttgtc
    15901 gccggtcagc acctgcggag agaggcactc aacgcctggt gtgccgcgcg cctgagcgac
    15961 gtttttggca aatttatctt caacgcgccc catgccggtt tcactgacgg caagttccgc
    16021 taaatctctg gcgtgttttt cgcctgcttc acgaatggca gcaatggcta actggcgcat
    16081 tgccacgctt tttaaccctt gctgggcgac tttggctgcc gcaacggcgt catccaggga
    16141 cgcgaaaacg cccatctcat gaacggcggc ggacggcgtg tcactgcttt gcattttcag
    16201 cagtaccgct ttcaccacct gttcaatatc ctgttgattc atgatgttct gccttatttg
    16261 tggaaaatta cctgaccgcc agacaccacc tcatcgacaa tgccaatcac gcacagatcg
    16321 accggtgacg tttcgctttt atgcgcctgg cgggcggaac tgccactcac cagtaacacc
    16381 cactccccgg ttcccgcgcc aatattgtcg atggcgacgg cgcattgccc gtcaggatta
    16441 ccttgtggat caatcatttc caccatcagc aatttgtcat gcgccagtcc gtgatggcgt
    16501 acggtacaaa caatttgtcc agtgacgact gccagtttca tacccgcctc cgtggcgtat
    16561 ttcaggtaaa agctccccct accctccgca gaaggtaaaa tgaaaaagga gagagcgtga
    16621 cgcccgaatc gacgtcacac agggtgatta caggttgctg ctatcgcctt tcaggccgat
    16681 cgggaagact tcttccaggt cgccgtgcgg acgcgggata acgtgtacag ataccagctc
    16741 gccgatacgc tgtgccgctg cggcacctgc atcggtcgcg gctttacagg ctgcaacatc
    16801 accacgaacc atggcagtac acaggccgcc gccaatctgc ttaacaccaa ccagcttgac
    16861 gcgcgcagct ttcaccatcg cgtcagaagc ctcaatcagt gcaaccaggc cccgggtttc
    16921 gatcattcct aatgcttcca ttgtgctttc ctctttatca gggtccagaa cgggaccgtt
    16981 cattcaacca gtgtttgtaa actgctttcg cggttcactt ctgtctgacg cggcacagct
    17041 gccaccagcg ccagctcgat aatttcctgc acgctacaac cacgagagag atcgtgcatc
    17101 ggcgcggcaa gtccttgtat cagtggcccg acggcacgat atccgccgag tcgttgtgcg
    17161 attttgtaac caatatttcc ggcttccagc gacggaaaaa ccatcacatt ggccttgccc
    17221 tgtagcgggc tggcaggcgc tttttgcgcc gccacttccg gcacgaaggc ggcgtcaaac
    17281 tgtaactcgc catccaccac cagctttggt gcgcgctcac ggacgatttc tgtcgcctgc
    17341 tggacgttag caacacaggg gtgacgggcg ctaccattgc tggaaaacga cagcatcgcc
    17401 acgcgcggct cttctccggt gatggcgcgc caggtttcgg cactggcaag cgcgatatcc
    17461 gccagctgcg ccgccgtcgg ctgtggcacc acgctgcaat cggcaaagcc caacgccggg
    17521 ccgctgtact gtggcaacat caggaaaatg gaagagagcg ttttacagcc tggctgcaag
    17581 ccgataatgc gtaatccggc acgcagcaca tttgccgtgg aagagaggtt gcccgcgata
    17641 cagacatccg ctttaccggc gctgaccatt gctgcggcga acatcagagg gtcggtaagt
    17701 ttttccagcg catccggcgg cgttttttcg cccgcgcggg ccagccagcg atgagcaaat
    17761 tcttcccgca ttgcgaggtt gccatgcgga tcaatcacct gtagcccgtc catcgccacg
    17821 ccgtgactga gcgcaaactg acgaagttca aacggattgg cgaccagaat gggcgttgcc
    17881 agaccttgtt gatgtaaata ttgcgcggct ttcagcacac gttggtctaa cgcatccgga
    17941 aaaaccactc tggcgggcgc tcgcaacgcc agttcacgac aacgttcaat aatcattgcg
    18001 tttcccccag tcgttgttgg atctgcttca ccgttagcgg ctgcttcgtt acgctcagga
    18061 tcatcatcac gtagaccgtg ctcgaaaggc ggttcagcgc ctgcaaaatg tccgggcgta
    18121 atacttcaaa gctgcgggtg ataaacacct gcgccgctac ggtttcggtt tcccgcactt
    18181 tggtacgtag cagattgagc aacgccgcat cgcgcccgtg gctggcttcc ggcaccagat
    18241 gatcgtggtc gagatagcgc agcggctgat gagaaagccg atgcagatct tcgtcagaaa
    18301 gcccgacaat cgcctggcag cccagcggtt cccccagcgc atcggcgcgc ataatgttgc
    18361 caagacgcga acggatatcc gctagccacg gctgccacgg ttccgccagt tcaatttgca
    18421 gccacactgc cagcgcaatg gtgctgtcga gaaccgcgcg aaaacccaga cgcggatcgc
    18481 ttttggcgac cattttttcc gccgacaggt gagtcagcgt atccggcttt tttgccaccg
    18541 gctggcggca cagttcgcag cacgcctgcg gatgttcatc gctactggtc agcccatgaa
    18601 cgggctgcgg ctgctgctgt tcatcgtcaa caaacaggcg gccctgctcg tcaataaact
    18661 tgatgcgcag atggcggctt tccagcaact cccgggcaga gggcgtcagg cgactgtccg
    18721 cagggagatg gatctctgcg ccttcgctga gcgtatggtt cgctcttagc catgcttcgg
    18781 tgatgaaatc tttcataggg attgccagtt agccggccag gcgacataca ggaatttcac
    18841 gctggatgtc gtaccaaatt cgatgctgga gcctttcggg ataaacatca cgtcgcctgc
    18901 tttggcgatc atggtttcgc cttcgtggcg aacatgtagc tccccttcca gcaccatgtc
    18961 gatttcgtcg tagttcaggg tccacgggaa gaatgcgttc tcccactgca taaaaccggc
    19021 ggccatgctg ctgccgtcgt ctccggtcac aagatcagtc aagcccacgc aatgcggctc
    19081 tgcgccgtcg aaacgaccaa acttgacgct gctgccatca atcactttga tgccgccttt
    19141 gccggtcacc gatttgaagc tcggctgcat tgcgccctgc tccagcgact gtttttcctt
    19201 catcactttt tccatcagct gtgcgaccag gctttcggta aactggcctt ccggcagctg
    19261 ggcaatgatg gtttcgcgga tgcgctggct ttcggttttg tcggctggca cgctggcggg
    19321 cacggacgcc gttaccggga tcgattcatc acattcggtg atggtaaaac ccagtagatc
    19381 cgccacttca cgggcttccg gggtaataat gctggcgcgc agaaccacgg acattgcctg
    19441 ttcgccgcgc gcgtgtgctt cacgaatatc attcgctgtg atgagttttt tcacctgctt
    19501 cctcctgttt ggtgagagat gccagataat ccaccagttg ctgcacgctt tgcgggtcgt
    19561 ggctgtttag ctcgaaaatc ggctcttcga agccagtctc aagcaataac tttcgcgttg
    19621 cggcgacgtc agcatctggc atgtccgttt tactgatgac ggcgatttgc cgtttgctga
    19681 cgccaatatc cagcaacccg gcaggcaacc gactttccgg gtcatttgcg ccatggacat
    19741 agatcagcat gtctacatcc tgtagcgtgg taattaaggc gtgataccag cggggatggc
    19801 taaaatattc acctggcgta tcaatatcgc ccttatcatt aaattccacg gcctgtgttt
    19861 ttctggcgag ggtataattc ccctgcaacg cattaaataa cgttgttttt cctgccccga
    19921 cgctacccac aaacgcaatt cgtttcatga atgcctcgta ttaacttttg gtcatttcac
    19981 aaagcgtata atttaataag cgacctaaac cgctgacggt ttgtgataac gcttcttcta
    20041 ctgcgcccac cgagccataa atcaccaatg caccgctgaa gcggtcgaga aaaccgatat
    20101 gcacatcagc ggctttcagc gctaaatcac cggcgatcat cgccgtttcg ccgggagtta
    20161 gcgtcataat gccaattgcg cccgcatccg gaacgccgat tttcttcgcc agttcctcac
    20221 cgggatgcgc gatgagatgc gccagtgtga cctgtttgcc gggcacaaat tcctgaatga
    20281 tgcgttcttt atccatgagt cgccgcctgt gaaaaacttt cgtgtatcgt gacaattttt
    20341 cgtcagagta aataacaaaa ttcggcaagc gcgtttaaaa ggtgaggtag atcactaaga
    20401 atggagagat attcgctgtg gtgcataaac tttcatatgc aacgcatgaa tataaataaa
    20461 ataatatcga gatattatat agactgcttt tttattattc gcaaactcag taataagctt
    20521 tactaatata ttgcagccaa accgcttacc ctgaatattc agggtaagcg tgagagttaa
    20581 aaaagaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc atgttgacga
    20641 tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca
    20701 ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg
    20761 cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca
    20821 tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca
    20881 tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg
    20941 ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa
    21001 aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt
    21061 cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc agcgcgttca
    21121 ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acgctaaaat
    21181 gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca
    21241 tgatcgcgcc gatcactgtc gggttactga tcagcgcccg ctgcgcctgt tcctgagtga
    21301 caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg
    21361 attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa
    21421 tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta
    21481 ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttctggcaga acaacgcgct
    21541 tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga
    21601 actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg
    21661 actccatcgc ggctttagcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg
    21721 gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac gctgaagcca
    21781 ccacttcgct ctgttcggcg tgggccagtt ccgcaatcgc gcgtaccgcc gccagtttca
    21841 tctcttcatt gatagccgtt gcgccaacat ccagcgcgcc acggaagata aacgggaagc
    21901 aaaggacgtt gttgacctgg tttggatagt cagaacgacc ggtgcaaatg atggcatccg
    21961 gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga
    22021 tcattggcgc acgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac
    22081 atccaaggaa aatatccgcg ccttcaatca catcatcgag ggtacgtttg ccgtcatcca
    22141 ccaccgcata tgcggctttg gtttccgcca tgtttggctc acggccctga tagataacgc
    22201 cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgctacc agcaggttca
    22261 tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt
    22321 tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct
    22381 gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac
    22441 attctggcgc tttaatgtct tcgaggttga ttccgccgaa ggtcggttcg agcgcagcga
    22501 caacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc
    22561 cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcca gccagcgcgc
    22621 caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc aggttaccgc
    22681 gcgcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa ggtgcggcaa
    22741 cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa
    22801 cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt
    22861 catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc
    22921 ctgcccacaa cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc
    22981 aaactctcac cattaatata atgttttggt aataatccta taacactgat gttacctgct
    23041 taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc
    23101 agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa
    23161 cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg atattgagtc
    23221 cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttac tcaaagctgc
    23281 cggattatca caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg
    23341 caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcagtca atttcggtgc
    23401 tgaaattctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac gcctctcttt
    23461 tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg
    23521 cgttgagaaa tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa ttcgcgcggc
    23581 cgaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttgccca
    23641 ggcgcgggcc tgtgcagaag cgggcgtttt tctgatttcg ccgtttgtcg ggcgtattta
    23701 tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt
    23761 taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat
    23821 gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac
    23881 tatcgcaccg aatttgctaa aggagctgca ggaaaaagtt tcgccagtgg tacgtaaatt
    23941 aatcccacct tctcagacgt tcccacgccc agctcccatg agcgaagcgg agttccgttg
    24001 ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc
    24061 cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt
    24121 gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgcggtac
    24181 aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc
    24241 tgtggaacga ttttcttaaa cataacccta ccgacccaac ctggtatgat cgcgaccgct
    24301 ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt
    24361 acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc
    24421 acccggagat tggttatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt
    24481 tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc
    24541 cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg
    24601 aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactgggc aagctgattg
    24661 gtttttacga tcacaacggt atttccatcg acggtgaaac tgaaggctgg tttaccgacg
    24721 atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg
    24781 atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc
    24841 tgattatctg ccgcacagta attggcttcg gttcgcctaa caaagcgggt aaggaagagg
    24901 cgcatggcgc accgctgggt gaagaagaag tggcattggc acggcaaaaa ctgggctggc
    24961 accatccgcc atttgaaatc cctaaagaga tttatcacgc ctgggatgcc cgcgaaaaag
    25021 gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc
    25081 aactggcaga agagtttacc cgtcggatga gcggtggttt accgaaggac tgggagaaaa
    25141 cgactcagaa atatatcaat gagttgcagg cgaatccggc gaaaatcgct acccgtaagg
    25201 cttcgcaaaa tacgcttaac gcttacgggc cgatgctacc ggagctgctc ggcggttcgg
    25261 cggatctggc tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc
    25321 cagcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg
    25381 gcatcgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat
    25441 acgcccgtaa cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata
    25501 cccacgactc aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg
    25561 ccagcctgcg cttaacgcca aatttcagca cctggcgacc gtgcgatcag gtggaagcgg
    25621 cggtgggctg gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa
    25681 ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg
    25741 gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag
    25801 agatggaaat taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg
    25861 tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg
    25921 tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt
    25981 acaagtatgt tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc
    26041 cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc
    26101 ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgat tgccggatgc
    26161 gacgctgacg cgtcttatcc ggcctacagc gtcttatccg gcctacatgt ccccgcaatt
    26221 ttgtttaacg ggtgatccac aacgtgggcc aggcgtctgg cccatgccag ttatcgcagg
    26281 tgggttctgc ggcataacgc accaggcgaa aacgctgacc gtcaaagcgc cagcgcgcct
    26341 gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca cgcgatttct
    26401 catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctgaccgttg ttgaacggca
    26461 aacgcaaccg aaccgggcgc gaggctagtg gctttttacg cgacacaatc catgccaaat
    26521 caatggtgtt ataggcccct gcctcacagc taatcatcat cagcgcttta tcatcagtca
    26581 gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga ccattcatcc
    26641 gccagtttcc ataatccagc aaatcgttgc gttcttcgag tgagagtggt gtcggcgttg
    26701 ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgagc ggcggttcgt
    26761 cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca tcaataaaca
    26821 acaacgccgc ttttaagcca ctcagagaaa tggtctgatt gccgtcgcgt aaggtgatcg
    26881 ccttcccttc ctgaatcatc tgcaaaaacg cggtgatggt tgccgtatca tcggttacta
    26941 ataaccatgg tgaaatccgc cacttgtcac cacttaacgc caacggctca ccatctaaca
    27001 gcagccgtgg cgctatctct ccttcagacg catccggcga cttcaacccg ccgcgctcaa
    27061 tacgtaaaac ggcatcggta tgcgccccgg cgctgcggct cagggtcatc accagtccat
    27121 tatgatcgcc cgtattgcgc gccacgcaga agttttggtt attacaggtg acctgccagt
    27181 cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac aaagcgaaaa
    27241 aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt caaacaaaca
    27301 gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcagatttgt cggattgagc
    27361 caataataaa caacgaaata ttattgttat ttttcagtcc attaaatgtg acgtttgcaa
    27421 atagttaagc aataacacag tcttaccgtc acgtatctcg ccggttttga tcatctccag
    27481 cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac cgccaccggc
    27541 gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct cagtcacacc
    27601 gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat agcccgtctc
    27661 ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca gcccggcgca
    27721 ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac ggaactgacg
    27781 aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac cattaccgcg
    27841 atcgtaaact tcacgtttat ggcggataac ttcgccatct ttgcgcgtga gatcgtaggt
    27901 aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga gggtgatttg
    27961 ttgcgtcata ccgactccac agcgcgaaat gaacaattat cttacgctgt gaagtcggtt
    28021 ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag tcaataatcc
    28081 cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca ccacgcacgg
    28141 cgtcaccacc agcgaagatt ttcggattgg tggtctggta acggtactgg ctttccacat
    28201 ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc cacggcatcc
    28261 cgtgcggatt gaagccaaac gccataatca ccgcgtcggc tggcatgaca aattcactgc
    28321 cttccaccgg cactggacgc cgacgcccct gggcatccgg ctcgccaaga cgcgtgcgca
    28381 ggaaacgaat cccgcagacg tgaccttgtt cattcagctc aagcgccacc ggctggacgt
    28441 taaattcgaa gttggccccc tcttcgcggg cgttcttcac ttctttcttc gagcctggca
    28501 tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg cgcagtgcgg
    28561 tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta agtccggcgg
    28621 tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta gtgttggcaa
    28681 tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa cccgctttca
    28741 tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa agcgaatcca
    28801 aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc gcgctgaaga
    28861 tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg ccgaaagtga
    28921 gcaagccacc gatttctgga tggcgatcgt acaccgtcac ccccacgcca ttgcgggtca
    28981 gaacatccgc acaggccagc cctgccggac ctgcaccgat aatcgccacc cgcttgtcca
    29041 ctttggtgac atggcttaag tcaggacgcc aacctttcgc caacgcctga tctgaaatgt
    29101 agcgttcaat gttgccgata gttaccgcgc cgtgctcatc gcgaatagta caggcacctt
    29161 cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg ttggtctggt
    29221 gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc cactgcggta
    29281 tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgcac ttaaggcagc
    29341 gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca tcaaaaccgg
    29401 ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt gcctgcattt
    29461 gttcgacttt actcattacc ggcatttctt gcgccgcggt actggcatgc cacggttgat
    29521 gttcctgacg cgcggtgcgc aagcggcggg atttcgccat gccggagaga gcgacgtcag
    29581 tgaccagttg cagcgcgtcc gccgggcaat tctcaacaca ggcaggaccg ttttcgcgcc
    29641 ccgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg acgggtgtca
    29701 ggacgatttg catcgtacca aaaggacagg ccaccacgca ggatttacag ccaatacact
    29761 tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta gggcagctac
    29821 gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc tgttgatgtt
    29881 tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc tcatcattgt
    29941 gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga ctgttggcca
    30001 taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt ctttatatga
    30061 gtgttgttat tacccgactt acaggggaat cggcaatgtt catgtgccca gaataagtaa
    30121 ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa ttgtgtttca
    30181 gcgaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt aaacgacccg
    30241 tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt ctttccacgg
    30301 gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc aatattgccg
    30361 gatcgctacg tatgcagagt taccgcctgg gctacgactt gcaaagtggc agtccacaac
    30421 tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta accaatctca
    30481 acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat gccaactggc
    30541 tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc aatattaata
    30601 attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct gaacgcaaaa
    30661 tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg ctggtctttt
    30721 ttactttgcg ccgtatacgc catcaggtgg ttgccccgct gaatcagctg gttaccgcca
    30781 gtcaacgtat tgaacacggg cagttcgact cgccgccgct ggataccagt ctgccgaatg
    30841 agcttggtct gcttgcaaaa acctttaacc agatgtcgag cgagctgcat aaattgtacc
    30901 tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgaggcc aagcgtcgtc
    30961 tggaggtgtt gtatcaatgt tcccaggcgc tgaacaccag ccagattgat gtgcattgtt
    31021 tccgccatat tttgcagatt gttcgcgaca atgaagcggc tgaatatctg gagttaaatg
    31081 tcggtgaaaa ctggcggatt agcgaagggc aaccaaaccc ggaattgccg atgcagattt
    31141 taccggtgac aatgcaagag acggtttacg gcgaactgca ctggcaaaat agtcacgttt
    31201 catcatcaga accgctgctt aacagtgttt catcgatgct gggacgcggt ttgtacttta
    31261 atcaggcgca gaagcatttt cagcagttat tgttgatgga agaacgtgca accatcgccc
    31321 gcgaattgca cgactcgctg gctcaggtac tttcttactt acgtatccag ttgacgttac
    31381 tgaagcgttc gataccggaa gataacgcca ccgcacaaag tatcatggcc gatttttccc
    31441 aggcgttgaa tgatgcttat cggcagttac gcgagctgtt gactactttc cgcctgacgc
    31501 tgcagcaggc ggatctcccc tccgcgttga gggaaatgct ggatacgtta caaaatcaaa
    31561 ccagcgccaa actgaccctc gactgccgtc tgccaaccct ggcgctggat gcgcaaatgc
    31621 aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa tgcgatgaag cacgccaacg
    31681 ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg caatcacacg gtttatatcc
    31741 gtgataacgg gattggtatc ggtgaaccga aagaacccga aggtcattat ggtctgaata
    31801 tcatgcgcga acgcgcggaa cggctgggtg ggacgctgac tttttcacaa ccttccggcg
    31861 gcggcacgtt agtgagtatt agctttcgct ctgcggaggg tgaggaaagt cagttgatgt
    31921 aatgcctcct actgaccaaa gaatacttgc acttaaggtt cagtataaaa gggcatgata
    31981 atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct acagtgaagc
    32041 aagtcaagcc tacaacgata cgcagaaaca cgaggtcctc ttttaatggc gaatttcttt
    32101 attgatcgcc ccatttttgc ctgggtgctg gcaatcctgt tgtgtctgac agggaccctg
    32161 gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa tgtgcgagtg
    32221 accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac ccaggttatc
    32281 gagcaaaata tgaccggcct cgataatctc atgtatatgt catctcagag cagtggcacc
    32341 ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga agccgtgcag
    32401 caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt gcaaaatcag
    32461 ggcgtgacgg tgcgtaaaac cggcgatacc aatattctga ccattgcttt cgtctctacc
    32521 gatggttcga tggataaaca ggatattgcc gattatgttg ccagtaatat tcaggacccg
    32581 ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata ttccatgcgt
    32641 atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga tgtcactgat
    32701 gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac accttccgtc
    32761 gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac accagaacag
    32821 ttccgcgata tcaccttgcg ggtgaatcaa gacggctcag aggtaaggct gggcgatgtc
    32881 gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa tggtaagcca
    32941 gcctccgggc tgggggtaaa actggcctcc ggcgcaaacg aaatggcgac agcggagctg
    33001 gtgctcaatc gtctcgacga gctggcgcag tacttcccgc acggactgga atacaaggtg
    33061 gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa aacgctgctg
    33121 gaagctatcg ctctggtttt cctcgttatg tatctgttcc tgcaaaactt ccgcgccacg
    33181 ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt actttacgcc
    33241 tttggttaca gcgtcaacac cttaaccatg ttcgcgatgg tgctggcgat cggtctgctg
    33301 gtggatgacg ccatcgtggt ggtggaaaac gtcgaacgta ttatgagtga ggaaggactc
    33361 actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact ggtcgggatt
    33421 gcgatggttc tttcggcggt atttgtacca atggccttct tcggcggcac caccggtgcc
    33481 atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt actggtagcg
    33541 atgatcctca ctccggctct gtgtgccaca ctacttaagc cactgaaaaa aggtgagcat
    33601 cacgggcaaa aaggcttttt tgcctggttt aaccagatgt ttaaccgcaa cgccgaacgc
    33661 tacgaaaaag gggtggcgaa aattctccac cgtagcctgc gctggattgt gatttatgtc
    33721 ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt cttaccgctg
    33781 gaagaccgtg gcatgtttac tacctcggta caattgccca gcggttcaac ccaacaacag
    33841 accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa agacaacatc
    33901 atgtcggtgt ttgccaccgt tggttctggc cctgggggca acgggcaaaa cgtggcgaga
    33961 atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac ctcgtttgcc
    34021 attatcgagc gtgcaacgaa ggcgtttaac caaattaaag aagctcgcgt tatcgccagc
    34081 agcccgccag caattagcgg tcttggtagt tctgcgggtt ttgatatgga gttgcaggac
    34141 cacgctggag caggtcacga tgcgctgatg gcagcacgta accagttgct agcgctggcg
    34201 gcggaaaacc cgaagctaac ccgtgtgcgc cataacggcc tcgatgacag tccgcagttg
    34261 cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgctatcga cgatattaac
    34321 gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga tcgcggtcgc
    34381 gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga tgacattaat
    34441 ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt cgcaacctca
    34501 cgctgggaga caggctcacc gcgtctggaa cgctataacg gttattctgc ggttgagatt
    34561 gttggagaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat ggaatcgtta
    34621 gtgaagcagt tgccaaatgg ctttggtctg gagtggacgg cgatgtcgta tcaggagcgg
    34681 ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtagt attcctgtgt
    34741 ctggcggcat tgtatgaaag ctggtcggta ccgttctcgg taatgctggt cgtgccgctg
    34801 ggggtaatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga cgtttacttc
    34861 caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct gatcgtcgag
    34921 tttgctaacg agatgaacca aaaaggccac gacctgtttg aagcgacgct ccacgcctgc
    34981 cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg cgtattgcca
    35041 atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac tggcgtaatg
    35101 ggcgggatga tttcagctac tattctggct atttacttcg tgccgctgtt ctttgtgctg
    35161 gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag caataaaaaa ggcgacatgc
    35221 caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt cttgctgttt
    35281 atactttgtc tcgggaatta cttacgaagc ataacttcga taaagtcttt ccagttcccc
    35341 agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg gaaacaatat
    35401 accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg acaaacctta
    35461 tgtaaatact atggtcctgc ggtgataatt tgtatgtgat acacagcaac atttcgagat
    35521 attcatacgg catctaatac tgatttaatt ctggttaaaa tacagacaga taacaagatg
    35581 aatattctta atgtttacgt taaaaatgtt taatatt
//
LOCUS       DAZFVO020000028        35570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFVO020000028 DAZFVO020000000
VERSION     DAZFVO020000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.028"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..2104)
                     /gene="glyS"
                     /locus_tag="ACHKNB_RS06720"
                     /old_locus_tag="pgaptmp_001344"
     CDS             complement(35..2104)
                     /gene="glyS"
                     /locus_tag="ACHKNB_RS06720"
                     /old_locus_tag="pgaptmp_001344"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGU4194897.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHCT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2114..3025)
                     /gene="glyQ"
                     /locus_tag="ACHKNB_RS06725"
                     /old_locus_tag="pgaptmp_001345"
     CDS             complement(2114..3025)
                     /gene="glyQ"
                     /locus_tag="ACHKNB_RS06725"
                     /old_locus_tag="pgaptmp_001345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGU4194898.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3120..3419)
                     /gene="ysaB"
                     /locus_tag="ACHKNB_RS06730"
                     /old_locus_tag="pgaptmp_001346"
     CDS             complement(3120..3419)
                     /gene="ysaB"
                     /locus_tag="ACHKNB_RS06730"
                     /old_locus_tag="pgaptmp_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HGU4194899.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3594..4589
                     /gene="wecH"
                     /locus_tag="ACHKNB_RS06735"
                     /old_locus_tag="pgaptmp_001347"
     CDS             3594..4589
                     /gene="wecH"
                     /locus_tag="ACHKNB_RS06735"
                     /old_locus_tag="pgaptmp_001347"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HGU4194900.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4631..5029)
                     /gene="yiaA"
                     /locus_tag="ACHKNB_RS06740"
                     /old_locus_tag="pgaptmp_001348"
     CDS             complement(4631..5029)
                     /gene="yiaA"
                     /locus_tag="ACHKNB_RS06740"
                     /old_locus_tag="pgaptmp_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HGU4194901.1"
                     /translation="MVSWIALVGGIVTYLLGLWNAEMQLNEKGYYFAVLVLGLFSAAS
                     YQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLSEKGFYGLAFFLSL
                     FGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHKNB_RS06745"
                     /old_locus_tag="pgaptmp_001349"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACHKNB_RS06745"
                     /old_locus_tag="pgaptmp_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HGU4194902.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHKNB_RS06750"
                     /old_locus_tag="pgaptmp_001350"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="ACHKNB_RS06750"
                     /old_locus_tag="pgaptmp_001350"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HGU4194903.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHKNB_RS06755"
                     /old_locus_tag="pgaptmp_001351"
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="ACHKNB_RS06755"
                     /old_locus_tag="pgaptmp_001351"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HGU4194904.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQHPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="ACHKNB_RS06760"
                     /old_locus_tag="pgaptmp_001352"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="ACHKNB_RS06760"
                     /old_locus_tag="pgaptmp_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4194905.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="ACHKNB_RS06765"
                     /old_locus_tag="pgaptmp_001353"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="ACHKNB_RS06765"
                     /old_locus_tag="pgaptmp_001353"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HGU4194906.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="ACHKNB_RS06770"
                     /old_locus_tag="pgaptmp_001354"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="ACHKNB_RS06770"
                     /old_locus_tag="pgaptmp_001354"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HGU4194907.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13865
                     /gene="xylR"
                     /locus_tag="ACHKNB_RS06775"
                     /old_locus_tag="pgaptmp_001355"
     CDS             12687..13865
                     /gene="xylR"
                     /locus_tag="ACHKNB_RS06775"
                     /old_locus_tag="pgaptmp_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HGU4194908.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13973..14797)
                     /gene="bax"
                     /locus_tag="ACHKNB_RS06780"
                     /old_locus_tag="pgaptmp_001356"
     CDS             complement(13973..14797)
                     /gene="bax"
                     /locus_tag="ACHKNB_RS06780"
                     /old_locus_tag="pgaptmp_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HGU4194909.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="ACHKNB_RS06785"
                     /old_locus_tag="pgaptmp_001357"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="ACHKNB_RS06785"
                     /old_locus_tag="pgaptmp_001357"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4194910.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="ACHKNB_RS06790"
                     /old_locus_tag="pgaptmp_001358"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="ACHKNB_RS06790"
                     /old_locus_tag="pgaptmp_001358"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HGU4194911.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /gene="ysaA"
                     /locus_tag="ACHKNB_RS06795"
                     /old_locus_tag="pgaptmp_001359"
     CDS             complement(18729..19202)
                     /gene="ysaA"
                     /locus_tag="ACHKNB_RS06795"
                     /old_locus_tag="pgaptmp_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HGU4194912.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHKNB_RS06800"
                     /old_locus_tag="pgaptmp_001360"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="ACHKNB_RS06800"
                     /old_locus_tag="pgaptmp_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HGU4194913.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20353..21351
                     /gene="yiaK"
                     /locus_tag="ACHKNB_RS06805"
                     /old_locus_tag="pgaptmp_001361"
     CDS             20353..21351
                     /gene="yiaK"
                     /locus_tag="ACHKNB_RS06805"
                     /old_locus_tag="pgaptmp_001361"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HGU4194914.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21363..21830
                     /gene="yiaL"
                     /locus_tag="ACHKNB_RS06810"
                     /old_locus_tag="pgaptmp_001362"
     CDS             21363..21830
                     /gene="yiaL"
                     /locus_tag="ACHKNB_RS06810"
                     /old_locus_tag="pgaptmp_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGU4194915.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21948..22421
                     /gene="yiaM"
                     /locus_tag="ACHKNB_RS06815"
                     /old_locus_tag="pgaptmp_001363"
     CDS             21948..22421
                     /gene="yiaM"
                     /locus_tag="ACHKNB_RS06815"
                     /old_locus_tag="pgaptmp_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HGU4194916.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22424..23701
                     /gene="yiaN"
                     /locus_tag="ACHKNB_RS06820"
                     /old_locus_tag="pgaptmp_001364"
     CDS             22424..23701
                     /gene="yiaN"
                     /locus_tag="ACHKNB_RS06820"
                     /old_locus_tag="pgaptmp_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HGU4194917.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAL
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23714..24700
                     /gene="yiaO"
                     /locus_tag="ACHKNB_RS06825"
                     /old_locus_tag="pgaptmp_001365"
     CDS             23714..24700
                     /gene="yiaO"
                     /locus_tag="ACHKNB_RS06825"
                     /old_locus_tag="pgaptmp_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HGU4194918.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24704..26200
                     /gene="lyxK"
                     /locus_tag="ACHKNB_RS06830"
                     /old_locus_tag="pgaptmp_001366"
     CDS             24704..26200
                     /gene="lyxK"
                     /locus_tag="ACHKNB_RS06830"
                     /old_locus_tag="pgaptmp_001366"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HGU4194919.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLIDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            26197..26859
                     /gene="ulaD"
                     /locus_tag="ACHKNB_RS06835"
                     /old_locus_tag="pgaptmp_001367"
     CDS             26197..26859
                     /gene="ulaD"
                     /locus_tag="ACHKNB_RS06835"
                     /old_locus_tag="pgaptmp_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGU4194920.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            26852..27712
                     /gene="sgbU"
                     /locus_tag="ACHKNB_RS06840"
                     /old_locus_tag="pgaptmp_001368"
     CDS             26852..27712
                     /gene="sgbU"
                     /locus_tag="ACHKNB_RS06840"
                     /old_locus_tag="pgaptmp_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGU4194921.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27706..28401
                     /gene="araD"
                     /locus_tag="ACHKNB_RS06845"
                     /old_locus_tag="pgaptmp_001369"
     CDS             27706..28401
                     /gene="araD"
                     /locus_tag="ACHKNB_RS06845"
                     /old_locus_tag="pgaptmp_001369"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGU4194922.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(28433..28534)
                     /locus_tag="ACHKNB_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /pseudo
     CDS             complement(28433..28534)
                     /locus_tag="ACHKNB_RS06850"
                     /old_locus_tag="pgaptmp_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28538..28801
                     /locus_tag="ACHKNB_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /pseudo
     CDS             28538..28801
                     /locus_tag="ACHKNB_RS06855"
                     /old_locus_tag="pgaptmp_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28748..29488)
                     /gene="yiaT"
                     /locus_tag="ACHKNB_RS06860"
                     /old_locus_tag="pgaptmp_001372"
     CDS             complement(28748..29488)
                     /gene="yiaT"
                     /locus_tag="ACHKNB_RS06860"
                     /old_locus_tag="pgaptmp_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HGU4194923.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            29612..30586
                     /gene="yiaU"
                     /locus_tag="ACHKNB_RS06865"
                     /old_locus_tag="pgaptmp_001373"
     CDS             29612..30586
                     /gene="yiaU"
                     /locus_tag="ACHKNB_RS06865"
                     /old_locus_tag="pgaptmp_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4194924.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(30583..31719)
                     /gene="yiaV"
                     /locus_tag="ACHKNB_RS06870"
                     /old_locus_tag="pgaptmp_001374"
     CDS             complement(30583..31719)
                     /gene="yiaV"
                     /locus_tag="ACHKNB_RS06870"
                     /old_locus_tag="pgaptmp_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4194925.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(31725..32048)
                     /gene="yiaW"
                     /locus_tag="ACHKNB_RS06875"
                     /old_locus_tag="pgaptmp_001375"
     CDS             complement(31725..32048)
                     /gene="yiaW"
                     /locus_tag="ACHKNB_RS06875"
                     /old_locus_tag="pgaptmp_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4194926.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            32219..32374
                     /locus_tag="ACHKNB_RS06880"
                     /old_locus_tag="pgaptmp_001376"
     CDS             32219..32374
                     /locus_tag="ACHKNB_RS06880"
                     /old_locus_tag="pgaptmp_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194927.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRP
                     LTIETRD"
     gene            complement(32593..34131)
                     /gene="aldB"
                     /locus_tag="ACHKNB_RS06885"
                     /old_locus_tag="pgaptmp_001377"
     CDS             complement(32593..34131)
                     /gene="aldB"
                     /locus_tag="ACHKNB_RS06885"
                     /old_locus_tag="pgaptmp_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HGU4194928.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(34296..35447)
                     /gene="yiaY"
                     /locus_tag="ACHKNB_RS06890"
                     /old_locus_tag="pgaptmp_001378"
     CDS             complement(34296..35447)
                     /gene="yiaY"
                     /locus_tag="ACHKNB_RS06890"
                     /old_locus_tag="pgaptmp_001378"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HGU4194929.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         8809 a         9151 c         8859 g         8751 t
ORIGIN      
        1 ctgccagatg gcaggctttt taataacggc gttattactg caacagcgaa atatccgcaa
       61 cgcgcaggaa cagttcgcgc agtttctcca gcatggtcag acggttgaga cgcaattctt
      121 tgtcatcaac catgaccatc actttatcga agaaagcatc aaccggctca cgcagctctg
      181 ccagttcgac cagcgcatcc tggtaacgac cttccgcaaa gtacggctcc agcttgtcac
      241 gtagcaccac aacctgcatc gccagtttaa tttcttccgg ctctttcagg gtagaggcat
      301 tcacgcggtc gctcagcact tcgtcagatt tcgccaaaat gttggagaca cgcttgttcg
      361 ccgccgccag tgcagcagct gcttcaaggg tacggaagtg cgataccgct ttcatgcggg
      421 catcgaaatc agccggacga gtcggacgac gcgccagtac cgcctggatg gtgtcaacgg
      481 tgtaaccttc gtcctgatac caggcgcgga agcgaccgag cataaagtcg ataacatcat
      541 caaccacgtt ggcgttagtc agcttatcgc catacagacg cactgcttct tcggtcagcg
      601 tttgcagatc aaggttgagg ttcttctcaa cgataattcg cagcacgcca agtgcggcac
      661 gacgcagcgc aaacgggtct ttgtcgcctt tcggatgctg gccgataccg aagatacccg
      721 ccagagtatc catcttgtca gcaatcgcca gcgcacaggc taccgggtta gacggcaggt
      781 catcaccggc aaagcgcggc tgatactgct cattcagcgc caccgcgaca tcttccgctt
      841 cgccatcgtg acgcgcatag tgcatcccca taacgccctg ggtgtcggtg aactcgaaga
      901 ccatgttggt catcaggtca cacttagaca gcaggcccgc acgggttgcg tggttaacgt
      961 cagcgccaat ctgttcagca atccagccag ccagcgcctg gatgcggtca gttttgtcgc
     1021 gcagcgtacc cagctgttgc tggaacagca cggtttgcag gcgcggcagg ttatcttcca
     1081 gacgtttttt acggtcggta ttgaagaaga actcggcatc cgccagacgt ggacgaacga
     1141 ctttctcgtt accagagata atttgctgcg gatctttcga ttcgatgttg gcaacgaaga
     1201 tgaagttcgg cagcagtttg ccatcgttcg catacaccgg gaagtatttc tggtcacctt
     1261 tcatggtgta aaccagggct tcagccggca ccgcgaggaa tttctcttcg aattttgcgg
     1321 tcagaacgac cggccactcc accagcgaag ccacttcttc cagcaggctt tcgcttaagt
     1381 cagcgttacc gccaatctta cgcgctgctt cttcggcatc ggctttaatc ttcgctttac
     1441 gttcttcgta atcggcgatg actttcccgc gctcacgcag aatttccgga tactgatcgg
     1501 cgttgtcgat ggtgaattcc ggttcgccca taaagcggtg gccgcgaatc acgcgatcgg
     1561 actgaatgcc cagaatggtt gccggaatga ctttgtcgcc cagcagcagg gtcacggtgt
     1621 gcaccggacg cacgaagtgc acgtcgcttg cgccccaacg catcagtttc gggatcggca
     1681 gtttcgccag agaagtcgca accatattcg gcagcagtgc ttcggtgctt tcgcccttca
     1741 catgggcgcg atacagcagc cattcgcctt tatcggtagt cagacgctca gcctggtcaa
     1801 cggtaatacc gcaaccacgc gcccaacctt ctgccgcttt gctcggtttg ccttcagcgt
     1861 cgaacgcctg ggcaatcgcc gggccgcgtt tttcgatttc acgatccggt tgcgcttccg
     1921 ccaggttagc tactttcagc gccagacgac gcggagcagc aaaccattga acggtgcagt
     1981 gtgcgaggcc agcgttatcc agctccgcag taaaattcgc agcaaaggac tcagccaggc
     2041 tgcgcagtgc ttttggtggc agctcttcag tgccgatttc caccagaaaa gttttctcag
     2101 acatagccgc ctcttactta tctttgttgc acatcgggaa gccgagggct tcacgggaag
     2161 cgtagtatgc ttctgccact gctttggtca gggtgcgaat gcgcagaatg tagcgctgac
     2221 gctcggtgac ggagatggct ttacgcgcat ccagcaggtt gaagctgtgg gcggctttca
     2281 gaatacgctc gtaggctggc agcggcagcg gattttccag cgccagcagc tgctgcgctt
     2341 ctttctcgta ctgctcaaag caggtgaaca ggaagtccac atccgcgtat tcgaagttgt
     2401 aagtggactg ctccacttcg ttctgatgga acacgtcgcc gtaggtggtt ttacccagcg
     2461 ggccgtcgct ccagaccagg tcgtaaacgc tgtctacgcc ctgaatgtac atggccagac
     2521 gttccagacc gtaggtgatc tcgccggtaa ccggtttaca ctccagacca ccaacctgct
     2581 ggaagtaagt gaactgcgtc acttccatgc cgtttagcca cacttcccag cccagtcccc
     2641 aggcacccag agtcgggttt tcccagttat cttccacgaa acggatgtcg tgaatagtcg
     2701 ggtccatgcc cagctctttc agagaaccga ggtacagctc ctgaatattg tccggcgatg
     2761 gcttaatgac cacctggaac tgatagtagt gctgtaaacg gttggggttt tcgccgtagc
     2821 gaccatcggt cgggcgacga gaaggctgaa cataagcagc cgccatcggt tctggcccca
     2881 gcgcgcgcag acaggtcatt gggtgagagg ttcccgcgcc gacttccatg tccaatggtt
     2941 gaacaatggt gcagccctgg cgagcccagt aatcctgtaa ggtcaggatc aagccctgga
     3001 aggtcctggt atcaaacttt tgcatattat ttcgtgctgg atacgtgtgg atttaaagga
     3061 agggatcagt atacccgctg gatggaagat atacagtacg aaacgggaaa aagcagggct
     3121 taacgcatgg aaagatgcaa aaaatggcca tctgcgtcaa aagaacagac aaactgctct
     3181 ttacggaagg tataaccgcg catttcatag cttccctgga actgttcgaa ggcggtgacg
     3241 tcgatttttt gctcgccggt gttataacgt tttgccgcct gatccttgca taacgcttcc
     3301 atattcaacg aacgcagagg atcaaccttt acccgttgtg ctttctgcac gggtgacggt
     3361 gtagaacaac ccactagcac cataagcgcc attgccggaa agaaagcgtt catcatcatt
     3421 aaattaccgt ctggtttgca ggtgagtctt attatttata tcggtatagt ggctgatatc
     3481 ttacgaattt ccttaagcct ctataagctg tagtgataaa actcagatta ttccagtgcg
     3541 ccaactgaat gatttatcgg tcagactttt tttaaccaga tacagagaca cagatgcagc
     3601 ccaaaattta ctggattgat aacctgcgag ggatagcgtg tttaatggtg gtgatgattc
     3661 acaccactac ctggtatgtg accaatgctc atagtgttag ccccgtcacc tgggatatcg
     3721 ccaatgttct gaattctgcc tctcgtgtca gcgtgccgct atttttcatg atttccggct
     3781 atctcttttt tggcgaacgc agcgcccagt cgcgccattt cttgcgtatc ggcttatgtc
     3841 tgatttttta tagcgcaatc gcactgctct acattgcgct gtttacctcc atcaatatgg
     3901 agttagcgct gaaaaacctg ctgcaaaagc cagtgtttta ccacttgtgg tttttcttcg
     3961 cgattgcggt gatttatctg gtttcaccgc tgattcaggt gaagaacgtc ggcggaaaaa
     4021 tgttgctggt gctaatggtg gtgattggca ttatcgctaa cccaaacaca gtgccgcaga
     4081 aaattgacgg ttttgaatgg ctgccaatta acttatatat caatggcgat actttttact
     4141 acattctgta tggcatgttg ggccgcgcta tagggatgat ggacacacag cataaagcac
     4201 tgtcgtgggt gagcgccgcg ctgtttgcga cgggggtttt tattatctct cgcgggacat
     4261 tatatgaatt gcagtggcgc ggaaattttg ccgatacctg gtatctttac tgtgggccga
     4321 tggtttttat ctgcgcaatc gcgctattga ctctggttaa aaacacgctg gatacgcgta
     4381 ccattcgcgg acttggctta atctcccgcc attcattggg tatatacgga ttccacgcct
     4441 tgattatcca tgcgctgcgc acccggggaa ttgagcttaa aaattggcca atactggata
     4501 ttatttggat cttttgcgcg acgttggcag cgagtttgtt actttctatg ctggtacaac
     4561 gaatcgacag aaacagatta gtgagttaag taaaagcccg ttcacattgg actgaccggg
     4621 cttacgtgaa ttattcgctg tattcttcct gggtaacctg tgtttctttt ggcgggttta
     4681 ttccggtatc acgaatattc ttctgcaccg ctactgcacc aaacaagctt aagaagaaag
     4741 ccagtccata aaaacctttt tcactgagta ataatgtcgc attccacaga cctaccatca
     4801 gtaatgcaac agagataata aagacagtca ggcaggtcat ataataaatg gaagtggtcg
     4861 gtatgccttc atacttgtcc cgaacggtct tttgataaga cgcagcagaa aacagtccta
     4921 acaccagtac ggcaaaataa tacccttttt catttaactg catctctgca ttccatagcc
     4981 ctaacagata ggtaacgata ccaccaacga gagctatcca tgacacaata ctaaaggccg
     5041 gtgaataggt tgattatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcgacc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc atataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 cagagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggcaatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttggtc ggttcttgcg gtttcggttc gataagcaac gtgccctgga
     7801 aaccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagc tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccgccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 aactgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg cctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg gtgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa tgtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttgc ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc agttgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatggaatga tgaaactggg taattcctcg
     8641 aagagaaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggcgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga taaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaga agccaaacaa gaaggtatta aagtattagc ttacgatcgt
     9181 atgattaacg atgcggatat cgatttctat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccctggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ccggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cgttgttggc aaatactgcc
     9661 gcagaaattg ccgttgagtt gggcaatggt caggaaccaa aagcagatac cacactgaat
     9721 aatggcctga aagatgtgcc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagaca cggtagttaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaatattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtctgc ttgcggttga atgctggcga aatcgtctca ctttgtgggg
    10021 aaaatgggtc tggtaaatca acactgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctgtcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgggcg agagttaacc gcgctttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcgcccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgctc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtatcgata
    11221 ttggcgcgaa atacgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgg gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttccggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtttccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctacc tgccagcact ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttgcagtct
    12061 ccggcctcta ccgcagtagt cggtcgccag gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat ggcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa catgcgcaaa
    13201 atcggctggc agactggcta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgggc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactc gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 ccatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgattaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacacg acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc aaggaaatgc aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgcgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgaccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gtgttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt ggggtttatc gcaaaattca gccgtttttt
    15001 gcgcgagatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagagacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc cagcaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacgcccctg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggcg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccggctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgag gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg gcaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc gccgcgcttc
    16561 gcgaatggaa aaaagctaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcaggataa agcaccgctc actctccaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgcaa ttacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aagcacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaatc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggcaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gcgcgcccgc tatggaatcc gaatatcgtg ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaagatcg ctgcctaatt cataaaccag aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcaactct accagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgccgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgc cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattat cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcactc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccct
    19321 ggtcgtccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctcc cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatattaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtattttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tcaagtttcg catattgaaa ttttaagcca aaaaagcgat caaaaaaaca
    20341 aggaagcctg ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
    20401 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
    20461 accgaatccg gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
    20521 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
    20581 gaacagtggg atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc
    20641 gccattgaac tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac
    20701 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
    20761 tggaccaact ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg cattggcact
    20821 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
    20881 atgttctctt acggcatgtt agaagttaac cgcctggcgg gccgtcagct cccggtcgat
    20941 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc
    21001 cgcattttgc cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc
    21061 gctactctcc tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa
    21121 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
    21181 cgcgatgcca agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa
    21241 aaccaggcca tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt
    21301 aacggtatta ccgttgatga cagcgtgtgg gccaaaatcc aggcgttata aggagataag
    21361 tcatgatttt tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa
    21421 aggcgctcga ttttctgcgc gctaccgatt tcaacgccct ggagccgggc gttgtcgaaa
    21481 tcgacggcaa aaatatttat gcgcaaatta tcgatttaac cacacgcgaa gcggtggaaa
    21541 accgtccgga agtgcatcgt cggtatatcg atattcagtt tctggcgtgg ggtgaagaga
    21601 aaatcggcat tgctattgat acgggaaata ataaagtcag cgaatcacta ttagagcagc
    21661 gcgatattat tttttatcat gacagtgaac atgaatcatt tatagaaatg ataccgggca
    21721 gctacgcaat tttctttccg caggatgttc accgaccagg ttgtattttg caaacagcct
    21781 ctgagatacg aaaaattgtg gttaaagtcg cattaacggc gctgaattaa tcgaataatc
    21841 gtctacgcca gaacgactgg gtaatgttat tgctctaact atattaataa tttaggttgc
    21901 tgcacatgca acttgaatta tgacagacat agcctcaagg aatagctatg aaaaaaatac
    21961 ttgaagcaat actggcgatt aatctcgccg tactttcctg tattgtgttt ataaacatta
    22021 ttttaagata tggttttcag acaagcattt tatctgtaga tgaattgtca cgttatttat
    22081 ttgtctggtt aacatttatt ggcgcgattg tggcttttat ggataacgcc cacgttcagg
    22141 tcacctttct ggtggagaaa ctctcccccg catggcaacg acgagtcgct ctggttaccc
    22201 attccttaat cttatttatt tgtggcgcac tggcctgggg cgcaacgcta aaaacaattc
    22261 aggactggag cgattattca ccgatcctcg gtttacccat cggcctgatg tatgccgctt
    22321 gtctgcccac cagcctcgtt atcgcattct ttgaactgcg tcatttatat caactcatca
    22381 cgcgtagcaa ttctctaacg tcgccaccgc aaggagctta atcatggctg tgctgatttt
    22441 tctgggctgt ctgttgggtg gtattgctat cggtttgcct attgcctggg cactgttgtt
    22501 gtgcggggcg gcgttaatgt tctggctgga catgtttgat gtccagatca tggcacaaac
    22561 gctggtgaac ggagccgata gcttctccct gctggcgatt ccgttctttg tgctggcggg
    22621 tgaaatcatg aatgcgggcg gcttgtcaaa acgcattgtt gacctgccga tgaaactggt
    22681 ggggcataaa ccaggcgggc tgggctacgt cggcgtgctg gcagctttga ttatggcgag
    22741 cctttctggt tctgccgttg cggataccgc cgccgttgcc gctctactgg tgccgatgat
    22801 gcgcagcgcc aactacccgg tcaaccgggc ggcggggctg attgcctctg gcggcattat
    22861 cgcgccaatt attccccctt ccattccgtt tattatcttc ggcgtttcca gcggattatc
    22921 catcagtaag ctgtttatgg cgggcattgc ccctggcatg atgatgggcg caacgctgat
    22981 gcttacctgg tggtggcagg ccagtcgcct taatttaccc cgccagcaaa aagcaacgat
    23041 gcaggaaatc tggcactctt ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat
    23101 tattggcggg tttcgctccg gtctgtttac ccctaccgaa gccggggccg ttgctgcttt
    23161 ctatgcgctg tttgtcgcca cagttattta ccgtgaaatg accttcgcca cactttggca
    23221 tgtactcatt ggcgcggcga aaaccacctc agtggttatg tttctggtgg cctcggcaca
    23281 agtttccgcc tggctgatta ccattgctga actgccgatg atggtttctg atttactgca
    23341 accgttggtc gattcaccgc gtctgctgtt tatcgtcatt atggtggcta tcctgattgt
    23401 cggcatggtc atggatttaa cgccgacggt attaattctt accccggtat taatgccttt
    23461 agttaaagag gcaggaatcg atccgattta tttcggtgtc atgtttatca ttaactgctc
    23521 aatcggctta attacaccgc ctatcggtaa tgtacttaac gttatttcag gggtggcaaa
    23581 actcaaattc gatgatgcag tcagaggcgt tttcccttac gtcctggttt tatattcatt
    23641 attagtcgta tttgttttta ttcccgatct catcatcctc cctttgaaat ggattaatta
    23701 aaaggaaaat attatgaaat tacgctctgt aacctacgca ttattcattg ctggcctggc
    23761 tgcattcagc acatcttctc tggcggcgca atctttacgt ttcggttatg aaacctcaca
    23821 aaccgactcg caacatattg cggcgaaaaa attcaatgat ttattgcagg agagaaccaa
    23881 aggcgagctg aaattaaaat tgtttccgga tagcaccctc ggtaacgcgc aggcgatgat
    23941 cagcggcgta cgtggcggca ccatcgatat ggaaatgtcc ggctcgaata actttgccgg
    24001 gttatcacca gtgatgaact tgcttgatgt ccctttcctg ttccgcgata ccgctcacgc
    24061 gcataaaacg ctcgacggca aagtcggtga tgatctgaaa gcctcacttg aaggtaaagg
    24121 actaaaagta ctggcctact gggaaaacgg ctggcgcgat gtcaccaact cgcgcgcacc
    24181 ggttaaaacc cccgccgacc tgaaagggct gaaaattcgc accaacaata gcccgatgaa
    24241 tatcgccgca ttcaaagtct ttggcgctaa cccgatcccg atgccatttg ccgaagtcta
    24301 taccgggctg gaaacgcgca ctatcgacgc tcaggaacac ccgatcaatg tcgtctggtc
    24361 agcaaaattt ttcgaagtgc agaagtacct ttctctgacg caccacgcct attccccgct
    24421 tctggtggtg atcaacaaag cgaagtttga tggcttaacc ccggagttcc agcaagcgct
    24481 aatttcatct gcgcaggaag cgggtaacta ccagcgcaaa ctggttgccg aagatcagca
    24541 aaaaatcatc gacggcatga aagaagcggg cgtggaagtc atcaccgatc tcgaccgcaa
    24601 agcctttagc gacgcactgg gtactcaggt ccgcgacatg tttgtaaaag atgttccgca
    24661 gggtgctgat ctactgaaag ccgtggatga ggtgcaataa accatgacgc aatactggct
    24721 ggggttagat tgtggcggta gctggctgaa agccgggctg tatgaccgcg aaggccggga
    24781 ggcaggcgtg cagcgcctgc cgctgtgcgc attaagcccg cagccaggtt gggcagagcg
    24841 cgatatggca gaactgtggc aatgctgcat ggctgtcatt cgcgaactgc ttacccattc
    24901 cggcgttagc ggggaacaaa ttgtcggtat cggtatctcc gcacaaggaa aaggcttgtt
    24961 tttgctggat aaaaacaaca agccgctcgg taacgccatt ttgtcctcgg atcgccgggc
    25021 gatggaaatc gttcgtcgct ggcaggaaga tggcatcccg gaaaaactct acccgttgac
    25081 ccgacaaacc ttgtggaccg ggcatccggt gtcgctgtta cgctggctga aagagcacga
    25141 accggaacgc tacgcgcaaa ttggctgcgt gatgatgacg cacgactacc tgcgctggtg
    25201 tttaaccggc gtcaaaggct gtgaagagag caatatttcc gagtccaacc tctacaacat
    25261 gagtcttggg gaatatgacc cgtgcctcat cgactggctg gggattgctg aaatcaatca
    25321 cgccctgccg cctgttgtcg gatctgccga aatctgcggg gagatcaccg ctcagacagc
    25381 cgtactgacc ggtctgaaag cgggtacgcc cgttgtcggc ggcctgtttg atgtggtttc
    25441 caccgcactc tgcgccggga tcgaagacga atttaccctt aatgcggtga tgggcacctg
    25501 ggcggtgacc agcggcataa cccgcggttt acgtgacggt gaagcgcatc cgtatgtcta
    25561 tggtcgctac gttaacgatg gtgaatttat cgttcacgaa gccagcccca cctcttccgg
    25621 caacctcgaa tggtttaccg cacagtgggg agaaatctcg tttgatgaga tcaatcaggc
    25681 cgttgccagc ttgccgaagg ctgggggcga tctctttttc ctgccgttcc tgtacggcag
    25741 caacgccgga ctcgagatga ccagtggttt ctacgggatg caggccattc acacccgcgc
    25801 gcacctgttg caggccatct atgaaggggt ggtgttcagc catatgaccc acctcaaccg
    25861 aatgcgcgaa cgttttactg atgttcacac cctacgcgtc actggcggcc cggcgcactc
    25921 cgatgtctgg atgcaaatgc tggcggacgt cagcggtctg cgtatcgagc tgccgcaggt
    25981 ggaagaaacc ggctgctttg gtgcggccct tgccgcccgc gtcggcaccg gggtttatca
    26041 caacttcagc gaagcccaac gtgacttgcg acacccggtg cgcaccctgc tgccagatat
    26101 gaccgcccat cagctttacc aaaaaaaata tcaacgttat cagcatctca ttgccgcact
    26161 tcagggcttt cacgcccgca ttaaggagca cacattatga gccgaccact tctgcaactg
    26221 gccctcgacc actcatcact tgaagccgcg cagcgcgacg tgacgctgtt aaaagacagc
    26281 gtcgatatcg tcgaagcggg caccattctc tgtttaaacg aagggcttgg cgcggtgaaa
    26341 gccttgcgcg aacagtgccc ggacaaaatc atcgttgctg actggaaggt cgccgacgct
    26401 ggtgaaacgc tcgcgcaaca ggcgtttggc gcaggcgcta actggatgac catcatctgc
    26461 gccgcgccgc tcgcgacggt agaaaaaggc cacgcaatgg cgcaacgctg cgggggtgaa
    26521 attcagatag agctgttcgg taactggacg ctggacgacg cccgcgactg gcatcgtatt
    26581 ggcgtgcggc aggccattta tcatcgcggt cgtgatgcac aggccagcgg gcaacagtgg
    26641 ggcgaagccg atctggcacg catgaaggcg ctttcagata tcggccttga gctttccatt
    26701 actggcggga ttacccctgc tgacctgccg ctgtttaaag atatccgcgt gaaagcgttt
    26761 attgccgggc gggcactggc aggcgcggca aatccggcgc aagtggctgg cgatttccat
    26821 gcgcaaatcg acgctatctg gggaggtgcg cgtgcgtaat catccgttag ggatttatga
    26881 aaaagcgctg gcgaaagatc tctcctggcc ggagcggctg gtgctggcaa aaagctgcgg
    26941 ttttgatttt gtcgaaatgt cggtggatga aaccgacgaa cggctctcac gtcttgactg
    27001 gagcgccgca caaaggactt cgctggttgc cgcgatgatc gaaacaggtg ttgggatccc
    27061 gtcgatgtgc ctgtccgcac atcgtcgctt tccctttggt agccgtgacg aggcagtgcg
    27121 cgaacgggcg cgggaaatca tgagtaaagc gattcgcctg gcgcgcgatc tcggcattcg
    27181 caccatccag ctggcaggtt atgacgtcta ttacgaagac cacgacgaag gcacccggca
    27241 acgttttgct gaagggctgg cgtgggcagt cgaacaggcg gcggcatcac aagtaatgct
    27301 ggcggtggag attatggata cagcgtttat gaactccatc agcaaatgga aaaaatggga
    27361 cgagatgctc gcctcgccgt ggttcaccgt ctacccggac gtcggcaacc tcagcgcctg
    27421 gggcaatgat gttcccgccg aactgaaact gggcattgac cgtatcgccg cgatccacct
    27481 gaaagacacc cagccagtca ccgggcaaag ccccggacag ttccgtgatg tgccgtttgg
    27541 cgaaggctgc gtcgatttcg ttggcatctt caaaacgctg catgaactga actatcgcgg
    27601 ttctttcctg attgagatgt ggaccgaaaa agccaaagag ccggtgctgg agatcattca
    27661 ggcgcggcgc tggattgaag cgcgtatgca ggaggctgga tttatatgtt agagcaactg
    27721 aaagccgacg tgctggcggc gaatctggcg cttcccgctc accatctggt gacgttcacc
    27781 tggggcaatg tcagcgcggt agacgacacg cggcaatgga tggtaatcaa accttccggc
    27841 gtcgagtacg acgtgatgac cgccgacgat atggtggtgg ttgagatagc cagcggcaag
    27901 gtggtggaag gtagcaaaaa gccctcgtcc gatacgccaa cgcatctggc actttaccgt
    27961 cgctatgccg aaattggtgg cattgtgcat acccactcgc gccacgccac catctggtca
    28021 caggccgggc tggatctccc cgcctggggc accacgcacg ctgattactt ctatggtgcc
    28081 atcccctgca cacgcctgat gaccgcagag gagatcaacg gcgagtatga ataccagacc
    28141 ggcgaagtga tcattgaaac tttcgaagaa cgcggcagga gtccggcaca aatcccggcg
    28201 gtactggtac attctcacgg cccgttcgcc tggggaaaaa atgccgccga tgcggtgcat
    28261 aacgccgtgg tgctggaaga gtgtgcctat atgggcctgt tctcgcgcca gcttgcgccg
    28321 cagctccctg cgatgcaaaa cgaactgctg gataaacact acctgcgtaa gcatggagac
    28381 aatgcctatt acgggcagta atccctcacg ccggggcttc atcgccccgg cactacgaat
    28441 tgatatgttc cttgctgtaa cgccgcttcc acgctgctgg cgttaaaccg gtgtgcttgc
    28501 gaaaaatctg ccggaaatag ccaacctcat taaaccgtat tgcatggagc aggccgacaa
    28561 cgtcgagtca ctgcataacc tgttgctgcc cgccgatgcg ccatttacga catttgaagg
    28621 caagggattg ttcagccata agatcctgat tcagacgcca ggttcccggt atgaacagag
    28681 caatccagag caacaacctt tgtggcatta cgacggagca ccagccgcac cgacatccac
    28741 cggtgaatta aaaacgccag ctcacgccgg tgacaaaggt aaaactctca ttacgatcaa
    28801 tcatcgggct gtcggtaatc tcttccggca gctcgctgta tccggcgctc gccatcaata
    28861 ctacgtgctc tcctatcggg tattttgccg tcaggctgac atagggcacc caggcatcct
    28921 gcgcggaata acttgccaga ccgctacgac gggactcact ctctgaaatg ccatagtaat
    28981 agtcactgaa attctcgtca taatagagaa cgcccagcgc aggtgtcagc gacaggcgac
    29041 ctatctgcat tttgtggaaa accgatagct cccccaccca gccgttgctg ttatccagaa
    29101 cgtccgcagc tgcagaggct ttcacggttc cccaacgctc gtggtgatac caggcaaccc
    29161 ccgccatagc cgtactatca cgcttatcaa gttgttgcat ggcatgatcg tcattatcgg
    29221 cagggtcaaa ttccagcggc atccaggatg cggtcaggct aagttcattt ttttcacttt
    29281 gcgacagaat aaaacctaac gtggtctgac gaacataaaa agaatcacct tcataactaa
    29341 tcaggggaat tgcctttgta ttttcattat aaccgcgata aggcgattca ttataagccg
    29401 caccggcacc aatggataat tcagaagcag ttgcgcttgc cataaaaggc aacgcaaata
    29461 acgccacaat attgcggtta attaacatgt ccggtattcc attttaaaat aagataaaaa
    29521 agagtcggca tagtaatgct tacaatttct ccggtgcaag attcatattt atatagtaat
    29581 atataaaatt atatataatt ggggtgttgc gatgacaaaa ttacaactta agtaccggga
    29641 gttaaaaatt atctcggtaa tcgctgccag tgaaaatatc agccatgccg cgactgtact
    29701 tggcatcgca caggccaacg tcagcaaata tcttgctgat tttgaatcaa aagtgggttt
    29761 aaaagtcttt gaccgaacca cccggcagct gatgctcaca ccttttggca ccgcgctgtt
    29821 accgtacatc aacgacatgc tggatagaaa cgagcaactt aataatttta ttgcagatta
    29881 taagcatgag aaacgtggtc gggtcactat ctacgcccca accggcataa ttacctattt
    29941 atccaaacat gtaatcgata aaattaaaga tatcggtgat atcaccttat cgctaaaaac
    30001 ctgcaatctt gagcgtaatg ccttttatga aggcgtcgaa tttcctgatg attgtgatgt
    30061 tttaatcagc tacgctccac caaaagatga atcgctggtt gccagtttta ttacccaata
    30121 tgccgtaacc gcttatgcca gccagcgcta tcttgagaaa catcctatta gccgtcctga
    30181 cgaactcgaa catcactcct gtattttgat cgactcgatg atgattgacg acgcgaatat
    30241 ctggcgcttc aacgtcgccg ggagtaaaga ggtgcgggat tatcgcgtga agggaaatta
    30301 cgtttgcgat aacacgcaat cggcgctgga gctggcacga aatcacctgg ggattgtgtt
    30361 tgcgccagac aaaagtgtgc agagcgacct gcaagacggc acgctggtgc cctgctttca
    30421 gcacccgtat gaatggtggc tggatctggt ggctatcttc cgtaaacggg aataccagcc
    30481 ctggcgggtc cagtatgtgc tggatgaaat gctgcgtgaa attcgccacc agcttgctca
    30541 gtcgcagcaa ctgcggcctg aacaagcggc ggaaagcgaa gattaatgat cgagataaag
    30601 gtaatgcacc caactggtca tgcgtaacag cactttgcgc atcaccgaga catggctgaa
    30661 atgatcagag tagaccgcca cctgggcgta aataccgtct ggtaaagcgc tcaaatcagt
    30721 gtgctcatcc agttcaatgg tcgcgataac gccatctgaa cccggcgctg tgtttaacgt
    30781 ctgtaaggtg ccggtcgact gataagctcc gccgggaaca gctggactaa tggctgccag
    30841 cttgccgctg aataccttac ctggcagagc gttaaatacc acttccgcat catcgccagg
    30901 ggccagtcgc agcaaggagt tctgacggaa ctgcgccaca atttgtcgtt tctgatcggg
    30961 tataaacacc atcaccggac gtagcggcag cgacgcggca taggtacccg gacgaatcag
    31021 cacctgggtc acataaccat cgctcggcgc acgcactatc gtctgctcaa ggttatattt
    31081 tgcttccgcg agctgcgctt tcaggctggc gatttgagaa tgttcaccca acaccaggct
    31141 atccagctgg ctctggatct gtttttgttc cgccgccgat gactttacgg aggcttcctg
    31201 cgccagataa ttttgccgcg ccacatcaat atcgcgttct gaaaacgggt ttactttcgc
    31261 ctggctgccg cgtgcgtaac gctgatactc tttagcgaat ttatcccgcg tggcctttgc
    31321 ctgctgagta ttcgccgcca tctcatctaa ctctgcgccc aacgcccgct gtttatgttc
    31381 tgcggtaacg atatccgcca tcagccgatc cacccgcgcc tgataacgcg tcgggtccag
    31441 tcgaaatagc acctcacctt ttttaatcag cgtatttttc ttatccgtca cttcgatcac
    31501 cacgcctgtc acctgtggga caacaggaat agaaataacc gctttttgcg ctttgaaggt
    31561 atacggatgg ttatagttca ttaacagaat taaaccactg acaataaata tgccacccag
    31621 ggccgctgtg ggaatggtcc atttatttac aggaattttg aagatcttaa acattgccca
    31681 tgcgaaagcc acataggtca aaataatcag taaatccata agtattactc ggcagaagag
    31741 gatttgatgt cggcgagctg tttctccagc ccggcaatac gctgttgtag ctgcacaacg
    31801 gaggagtcat ggttttgcat tccccatccc cgctccgggc ggtaaagcgt ggcccaaatc
    31861 cacaaaaatg gccagataac atgtaacgta aaaagactca cccaaccagc aacatgaatg
    31921 gcgtcggcat gaggatgatt acgtttttta gcaatcaggt agggaatatc atgtaaaata
    31981 ataatcccat agaagatcac cagaaataca aaaataagca ctcccagtgc aaaatagtcc
    32041 aggaacatat ttccctcaga gaatataaaa aagaacaatt aacgcatatt atgaaatgcc
    32101 atgatgcagt gcaatttcct tatttatata acgacatatt gttttttata tactttctga
    32161 agtgtgctac ctggcttttc aacaggtcga gacttatcat attgaaataa tggaactatt
    32221 ggccaaacta atgaataact ccagtaaaac atccacagta cagattaagc gtattaaacc
    32281 ttcaattatc taccgtttat tgctgattgg cctcggatca ccaatggtga tttacggcct
    32341 gattcgcccg ctcaccatcg aaacgcgaga ttaaactcct gacaaaacac gccccagaaa
    32401 cgcacttctg ttggcgtagt ctgggttatt gcgcacgtag gtttaaaacg taaattgtaa
    32461 caacgtgcgc tttgtttatg ccggatgcgg cgtgtacgcc ttatccggcc tacggtcagg
    32521 ttcccgtagg catgataaga cgcgtaagcg tcgcatcagg caatgaatac ccaatgcgac
    32581 cagcttctta tatcagaaca gccccaacgg tttatccgag tagctcacca gcaggcactt
    32641 ggtttgctgg taatgctcca gcatcatctt gtgggtttcg cgaccgatac ctgattgttt
    32701 gtagccacca aacgcagcat gtgccgggta agcgtgatag cagttggtcc acacgcgccc
    32761 ggcctgaatg ccgcgcccca tcttataggc cagattaccg ttacgactcc agacgcctgc
    32821 gcccaggcca tattgcgtat cgttcgccag ctccagcgct tcttccatcg ttttgaaggt
    32881 ggtcaccgcc agcaccgggc caaaaatctc ctcctggaac acccgcatat tgttctgacc
    32941 aaacagaatc gtcggttcga ggtagtagcc gtctttcagt tcaccttcca gcagcttgcg
    33001 ccgcccgccg gtgagcacgt ctgcgccctc ttttttaccg atatcaatgt agttgaggat
    33061 ggtttccagt tgcccgtgag aaacctgcgc gcccatttgc gtcacgctgt cgagtgggtt
    33121 accgctgcga atgctttcga cacggcggat ggcgcgttcc ataaagcgtt cgtagattga
    33181 ctcctgcact aaggcacgac tcggacaggt gcaaacttca ccctggttaa aggcaaacag
    33241 tgcaaagcct tccagcgctt tatcgaaaaa ggcatcttct tcatccatca catcagcaaa
    33301 gaagatattt ggcgatttgc cgcccagctc cagcgtcacc ggaataatgt tttgcgtggc
    33361 gtattgcata atttgttggc ccacttccgt tgagccggta aacgccactt tggcgatgcg
    33421 tttcgaggtc gccagatatt cgccaatttc cccacctgcg ccgttgacca cgttcaccac
    33481 gcccggcggc agtaaatcac cgacgatttc cattagcagc agtacagaaa gcggggtaag
    33541 acgtgcgggt ttcagcacca cacagttgcc cgccgccagc gcgggagcca ttttccagct
    33601 cgccatcagc agcgggaagt tccacgggat aatctgcccc accacgccta acggttcatg
    33661 gaaatgatag gccacggttt cgctatcaac ttcactgatc ccaccttcct gcgcccgaat
    33721 acacgaggcg aaatagcgga aatggtcaat cgccagcggt acatccgcag cactggtttc
    33781 gcgaatgggt ttgccgttat cccaggtttc agctgtcgct aacagctcga ggttttgttc
    33841 cattcgatcg gcaatcttaa acagaatcgc cgcgcgatcc tgcaccgagg tgtgcgccca
    33901 tttatctttc actttgtgcg cagcatccag cgccagatcg atgtctcgtt tgcccgaaga
    33961 cgccacttcg cacagcagct gcccggtcac cggcgtcaga ttctggtaat actcgccgtc
    34021 ggcaggggct acccattcgc cgccaataaa gttgtcatag cgggctttta acttgagggg
    34081 gaaaccatac tcgccgggct taatctgtgc tgaaggggga ttattggtca tgatcgtctc
    34141 cttgcggtgt gaggtataac aagggtagac gtgactggcg aaatcttcgc cagtcggtaa
    34201 cagctttacg acagctatca cgaatttacg ggcaagggtt acagagatag ttgaaaaagc
    34261 ggctgacaat ttgccagccg ttgtggaaat gatgattaca tcgctgcgcg ataaatcgcc
    34321 acaatttctt cgtgagttgc ctggatcggg ttagtaaaac cacaggcatc tttcagggca
    34381 ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
    34441 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttccgca
    34501 tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac agtcacgcag acgtgcggcg
    34561 gcgactttgc tgttgaatac ctgaacatgc ggcagcaaaa cggcgttaca tacaccgtgt
    34621 ggcaggttgt agaaaccgcc cagctggtgc gccatcgcat gaacataacc cagagaagca
    34681 ttattgaacg ccataccggc gaggaactgg gcataagcca ttgcttcacg cgctttcgca
    34741 ttactgccat cttcaacggc taacggcagg ttttcggcaa tcatggttac ggctttcagt
    34801 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
    34861 aaggcatcca tacccgttgc ggcggtcagt gacttcggca taccaatcat cagagaggag
    34921 tcattgacag aaagcagcgg agtgacatgt ttatcaacaa tcgccatttt gatatgacgc
    34981 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtattg
    35041 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata
    35101 tcgccgccat tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
    35161 cctaaggaga tcacgctatc gcaattattc tctttaagta atttcaaacc tgcggcgacg
    35221 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
    35281 tccagtgctt tttgcacatc gcccgccata cctaatttcg ttaacatact gtcagtgaca
    35341 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcagt caatgaatca
    35401 gcgccgatga cattcacaga aggaataaag aaagttgaag atgccataac acactcctga
    35461 aagtgtaaaa aagggtgggc agcttactga ggattttcat tcgaaaacat gatcattatt
    35521 attgattatc aaaattaatc taataaaaag cacattgtat tcattaaaca
//
LOCUS       DAZFVO020000029        35241 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFVO020000029 DAZFVO020000000
VERSION     DAZFVO020000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.029"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..884)
                     /locus_tag="ACHKNB_RS06895"
                     /old_locus_tag="pgaptmp_001379"
     CDS             complement(<1..884)
                     /locus_tag="ACHKNB_RS06895"
                     /old_locus_tag="pgaptmp_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194930.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTETLA
                     AARTHSDENDKRTLVSANT"
     gene            1116..2606
                     /locus_tag="ACHKNB_RS06900"
                     /old_locus_tag="pgaptmp_001380"
     CDS             1116..2606
                     /locus_tag="ACHKNB_RS06900"
                     /old_locus_tag="pgaptmp_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGU4194931.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDASPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDISGLVQENGHA"
     gene            2629..4080
                     /locus_tag="ACHKNB_RS06905"
                     /old_locus_tag="pgaptmp_001381"
     CDS             2629..4080
                     /locus_tag="ACHKNB_RS06905"
                     /old_locus_tag="pgaptmp_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HGU4194932.1"
                     /translation="MKTECSDVRTDIPGETVPEGYFTPLPADVLVSTPFRKRCLKQLW
                     ENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQFTTYAVRLAR
                     GRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRCWQPGISVPEG
                     VYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAGAVWHQHPEMA
                     LIRTVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQPLPVVPSPVED
                     AENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPDKESSIELPEE
                     SGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLSVNSVDSNVHV
                     VSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFFQARLYSDAEH
                     SGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            4148..5185
                     /locus_tag="ACHKNB_RS06910"
                     /old_locus_tag="pgaptmp_001382"
     CDS             4148..5185
                     /locus_tag="ACHKNB_RS06910"
                     /old_locus_tag="pgaptmp_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4194933.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(5381..5456)
                     /locus_tag="ACHKNB_RS06915"
                     /old_locus_tag="pgaptmp_001383"
     tRNA            complement(5381..5456)
                     /locus_tag="ACHKNB_RS06915"
                     /old_locus_tag="pgaptmp_001383"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5421..5423),aa:Phe,seq:gaa)
     gene            complement(5563..6138)
                     /locus_tag="ACHKNB_RS06920"
                     /old_locus_tag="pgaptmp_001384"
     CDS             complement(5563..6138)
                     /locus_tag="ACHKNB_RS06920"
                     /old_locus_tag="pgaptmp_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4194934.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPDHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(6175..7872)
                     /gene="dsbD"
                     /locus_tag="ACHKNB_RS06925"
                     /old_locus_tag="pgaptmp_001385"
     CDS             complement(6175..7872)
                     /gene="dsbD"
                     /locus_tag="ACHKNB_RS06925"
                     /old_locus_tag="pgaptmp_001385"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HGU4194935.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITSEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVTNNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILVLPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(7848..8186)
                     /gene="cutA"
                     /locus_tag="ACHKNB_RS06930"
                     /old_locus_tag="pgaptmp_001386"
     CDS             complement(7848..8186)
                     /gene="cutA"
                     /locus_tag="ACHKNB_RS06930"
                     /old_locus_tag="pgaptmp_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HGU4194936.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(8302..9603)
                     /gene="dcuA"
                     /locus_tag="ACHKNB_RS06935"
                     /old_locus_tag="pgaptmp_001387"
     CDS             complement(8302..9603)
                     /gene="dcuA"
                     /locus_tag="ACHKNB_RS06935"
                     /old_locus_tag="pgaptmp_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HGU4194937.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(9721..11157)
                     /gene="aspA"
                     /locus_tag="ACHKNB_RS06940"
                     /old_locus_tag="pgaptmp_001388"
     CDS             complement(9721..11157)
                     /gene="aspA"
                     /locus_tag="ACHKNB_RS06940"
                     /old_locus_tag="pgaptmp_001388"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HGU4194938.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            11494..11970
                     /locus_tag="ACHKNB_RS06945"
                     /old_locus_tag="pgaptmp_001389"
     CDS             11494..11970
                     /locus_tag="ACHKNB_RS06945"
                     /old_locus_tag="pgaptmp_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HGU4194939.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(11986..13242)
                     /gene="yjeH"
                     /locus_tag="ACHKNB_RS06950"
                     /old_locus_tag="pgaptmp_001390"
     CDS             complement(11986..13242)
                     /gene="yjeH"
                     /locus_tag="ACHKNB_RS06950"
                     /old_locus_tag="pgaptmp_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HGU4194940.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLTSEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            13518..13811
                     /locus_tag="ACHKNB_RS06955"
                     /old_locus_tag="pgaptmp_001391"
     CDS             13518..13811
                     /locus_tag="ACHKNB_RS06955"
                     /old_locus_tag="pgaptmp_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGU4194941.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13855..15501
                     /gene="groL"
                     /locus_tag="ACHKNB_RS06960"
                     /old_locus_tag="pgaptmp_001392"
     CDS             13855..15501
                     /gene="groL"
                     /locus_tag="ACHKNB_RS06960"
                     /old_locus_tag="pgaptmp_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGU4194942.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            15639..15992
                     /locus_tag="ACHKNB_RS06965"
                     /old_locus_tag="pgaptmp_001393"
     CDS             15639..15992
                     /locus_tag="ACHKNB_RS06965"
                     /old_locus_tag="pgaptmp_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HGU4194943.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(16195..17064)
                     /locus_tag="ACHKNB_RS06970"
                     /old_locus_tag="pgaptmp_001394"
     CDS             complement(16195..17064)
                     /locus_tag="ACHKNB_RS06970"
                     /old_locus_tag="pgaptmp_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4194944.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(17454..18482)
                     /gene="epmB"
                     /locus_tag="ACHKNB_RS06975"
                     /old_locus_tag="pgaptmp_001395"
     CDS             complement(17454..18482)
                     /gene="epmB"
                     /locus_tag="ACHKNB_RS06975"
                     /old_locus_tag="pgaptmp_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4194945.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            18524..19090
                     /gene="efp"
                     /locus_tag="ACHKNB_RS06980"
                     /old_locus_tag="pgaptmp_001396"
     CDS             18524..19090
                     /gene="efp"
                     /locus_tag="ACHKNB_RS06980"
                     /old_locus_tag="pgaptmp_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4194946.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            19142..19267
                     /gene="ecnA"
                     /locus_tag="ACHKNB_RS06985"
                     /old_locus_tag="pgaptmp_001397"
     CDS             19142..19267
                     /gene="ecnA"
                     /locus_tag="ACHKNB_RS06985"
                     /old_locus_tag="pgaptmp_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4194947.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            19378..19524
                     /gene="ecnB"
                     /locus_tag="ACHKNB_RS06990"
                     /old_locus_tag="pgaptmp_001398"
     CDS             19378..19524
                     /gene="ecnB"
                     /locus_tag="ACHKNB_RS06990"
                     /old_locus_tag="pgaptmp_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4194948.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            19699..20016
                     /gene="sugE"
                     /locus_tag="ACHKNB_RS06995"
                     /old_locus_tag="pgaptmp_001399"
     CDS             19699..20016
                     /gene="sugE"
                     /locus_tag="ACHKNB_RS06995"
                     /old_locus_tag="pgaptmp_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4194949.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(20013..20546)
                     /gene="blc"
                     /locus_tag="ACHKNB_RS07000"
                     /old_locus_tag="pgaptmp_001400"
     CDS             complement(20013..20546)
                     /gene="blc"
                     /locus_tag="ACHKNB_RS07000"
                     /old_locus_tag="pgaptmp_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4194950.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(20635..21768)
                     /locus_tag="ACHKNB_RS07005"
                     /old_locus_tag="pgaptmp_001401"
     CDS             complement(20635..21768)
                     /locus_tag="ACHKNB_RS07005"
                     /old_locus_tag="pgaptmp_001401"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4194951.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(21831..22190)
                     /gene="frdD"
                     /locus_tag="ACHKNB_RS07010"
                     /old_locus_tag="pgaptmp_001402"
     CDS             complement(21831..22190)
                     /gene="frdD"
                     /locus_tag="ACHKNB_RS07010"
                     /old_locus_tag="pgaptmp_001402"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4194952.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(22201..22596)
                     /gene="frdC"
                     /locus_tag="ACHKNB_RS07015"
                     /old_locus_tag="pgaptmp_001403"
     CDS             complement(22201..22596)
                     /gene="frdC"
                     /locus_tag="ACHKNB_RS07015"
                     /old_locus_tag="pgaptmp_001403"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4194953.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(22607..23341)
                     /gene="frdB"
                     /locus_tag="ACHKNB_RS07020"
                     /old_locus_tag="pgaptmp_001404"
     CDS             complement(22607..23341)
                     /gene="frdB"
                     /locus_tag="ACHKNB_RS07020"
                     /old_locus_tag="pgaptmp_001404"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4194954.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(23334..25142)
                     /gene="frdA"
                     /locus_tag="ACHKNB_RS07025"
                     /old_locus_tag="pgaptmp_001405"
     CDS             complement(23334..25142)
                     /gene="frdA"
                     /locus_tag="ACHKNB_RS07025"
                     /old_locus_tag="pgaptmp_001405"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4194955.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            25467..26444
                     /gene="epmA"
                     /locus_tag="ACHKNB_RS07030"
                     /old_locus_tag="pgaptmp_001406"
     CDS             25467..26444
                     /gene="epmA"
                     /locus_tag="ACHKNB_RS07030"
                     /old_locus_tag="pgaptmp_001406"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4194956.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            26663..28165
                     /gene="yjeM"
                     /locus_tag="ACHKNB_RS07035"
                     /old_locus_tag="pgaptmp_001407"
     CDS             26663..28165
                     /gene="yjeM"
                     /locus_tag="ACHKNB_RS07035"
                     /old_locus_tag="pgaptmp_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4194957.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            28217..28531
                     /gene="yjeN"
                     /locus_tag="ACHKNB_RS07040"
                     /old_locus_tag="pgaptmp_001408"
     CDS             28217..28531
                     /gene="yjeN"
                     /locus_tag="ACHKNB_RS07040"
                     /old_locus_tag="pgaptmp_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGU4194958.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLLDIYCAYLIRCLVFRGELVGYGDLFHMRYSEIKRPV
                     TL"
     gene            28528..28842
                     /locus_tag="ACHKNB_RS07045"
                     /old_locus_tag="pgaptmp_001409"
     CDS             28528..28842
                     /locus_tag="ACHKNB_RS07045"
                     /old_locus_tag="pgaptmp_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGU4194959.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDIRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(28873..32196)
                     /gene="mscM"
                     /locus_tag="ACHKNB_RS07050"
                     /old_locus_tag="pgaptmp_001410"
     CDS             complement(28873..32196)
                     /gene="mscM"
                     /locus_tag="ACHKNB_RS07050"
                     /old_locus_tag="pgaptmp_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4194960.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(32218..33186)
                     /gene="asd"
                     /locus_tag="ACHKNB_RS07055"
                     /old_locus_tag="pgaptmp_001411"
     CDS             complement(32218..33186)
                     /gene="asd"
                     /locus_tag="ACHKNB_RS07055"
                     /old_locus_tag="pgaptmp_001411"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4194961.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMANLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(33283..34335)
                     /gene="rsgA"
                     /locus_tag="ACHKNB_RS07060"
                     /old_locus_tag="pgaptmp_001412"
     CDS             complement(33283..34335)
                     /gene="rsgA"
                     /locus_tag="ACHKNB_RS07060"
                     /old_locus_tag="pgaptmp_001412"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4194962.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            34430..34975
                     /gene="orn"
                     /locus_tag="ACHKNB_RS07065"
                     /old_locus_tag="pgaptmp_001413"
     CDS             34430..34975
                     /gene="orn"
                     /locus_tag="ACHKNB_RS07065"
                     /old_locus_tag="pgaptmp_001413"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4194963.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(34988..>35241)
                     /locus_tag="ACHKNB_RS07070"
                     /old_locus_tag="pgaptmp_001414"
     CDS             complement(34988..>35241)
                     /locus_tag="ACHKNB_RS07070"
                     /old_locus_tag="pgaptmp_001414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4194964.1"
                     /translation="TRDPDLGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFP
                     SATPQKSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT         8422 a         8732 c         9404 g         8683 t
ORIGIN      
        1 gtattcgcac tgaccagtgt tcttttgtcg ttttcatcgc tgtgcgttct ggcagcagcc
       61 agagtttcag tatcgcccgc ggtgcgtgcc tggcgttcgg tcgtcagtaa tccgtcagta
      121 cggttattga tggcggtttc tttctggtca gcataggtat tcgcactgac cagtgttctt
      181 ttgtcgtttt catcgctgtg ggctctggct gcagccagcg ctgctgcatc accttctgtt
      241 cgggccctgg tttcagttat gacctgacca gagagggtat taagctgatt ctgcaggttg
      301 tttgttgatt ttattctttc tccagcttcc cggttaacag cactggtcat ctgcatcaga
      361 tttaccgcat catggtcatc tttcccctgt gccagatttg tgatgcggcg tttcagtgtg
      421 tcagagccga cggatacgat attgtttccg ttagcactgg aatgactgcc cagtgccacg
      481 gagtcgttcc cggttacgga cgatgtcaga cctatggcaa cagatgaatc accgttaatt
      541 ctggtggcat taccgaggct gacgctgtaa ccatccagca caatatcttc gcccactttt
      601 ctgatcaaac cggaagcttt gaccgtggag gaattaccaa taacaatgtt gttataccct
      661 ccctccacgg aattactgtt acccacaacc agactgtttg actctgatgt gacagtattt
      721 tcactgccgt aaatatttcc ggagaatcgt gttgttgtat tgtttttttc tccggtgatg
      781 ttcggtgtgt tgacatcagc atgtgctgtc tgacagacga tggcggctga cagcatagct
      841 gcaactgtta atgaataaga aacgtgtttt tttacttcgg acattatttt ttcctgccag
      901 attatgacct ttcaggtccc ggtatcctgg ataagcgcgg cagacaggag accacctgcc
      961 tttctgagga caggatgaga agcatccggt gtgatggcga tgattaattc atcatatgac
     1021 cagggaaatt tttctgttgc tgtacagagt tatttgtggt gggcataccc ctgatgcatc
     1081 agattacgga aatgtttttc cggagaagag aatacatgtc ttttaatccc acagatgaac
     1141 agtacgccat tattaactgg caggggcagc aactggttgt gaatgcgttt tccggcacag
     1201 ggaagacgag tacgctggta cagtttgccc gggccagtcc ggacagccgt atgctctatc
     1261 ttgcctataa ccgggcaata cgtgatgagg cagagcgtaa atttcccttt aacgtggaat
     1321 gcagaacgtc acatcagctg gcgtatgccc gggttggaag acactaccgt catcgtctgg
     1381 ttccgggatt acgtattact gatgtggcga gaaagctgaa tacacgatac tgggctctgg
     1441 cacgggtggc agggacgggg ctgaatcact ttatctgttc tgccgatgcg gtaccgggcc
     1501 tgcatcacct gccggataag gatgagatgc ggggtgttcc gccagcagat gcgcttcggg
     1561 cggtgcagtt gctctggaac gaaatgagca accccggggg gacgttcccg gtgacgcacg
     1621 atacttatct gaaactcttc cagttgtccg gtgcagacct gtctcaccga tgggacacca
     1681 tcctgtttga tgaggcgcag gatgcgagtc cggtgaccac tgctctggtt ctggggcaga
     1741 agtgtcgggt ggtccttgtt ggagattgtt atcaacagat ttaccgcttt cggggtgcga
     1801 ataatgcgct ttcccatcct gcactgaaga atgcggaccg gttgtggctg acacagagtt
     1861 tccgttttgg tccggcggtg gctcgtatgg caaatctgct tctgcagcgt gaaggtgaaa
     1921 cacgagaggt gaaaggttgt ggtggtgatg atgaggtatt gctgaaatgc catgcgcgtg
     1981 aacatctgca ggggcattac acggtactca gtcgtactgt cgcgggtgtg attgctactg
     2041 cgctgatggc ggcaatgaaa gggcaaaagg tttactgggt ggggggaatc gaaggttaca
     2101 ggaccgggga acttgaggat ctctactggt tccaggttga tatgccggaa cggatgcatt
     2161 cagacaggct gcgccgggac tacaggaatt ttgaagaata taaatatatt gcaaaatcca
     2221 cgaaggatgt ggagatgaat cagtcccttc ggcttctgga gttgtgtttt ccgttaccga
     2281 aaaaactgga acttctccgg cagtatacgg taacgaatga gcaggatgca gatatcacgg
     2341 tatcaacggc acaccggagc aaggggctgg agtggccggt tgtaatgctg gatgaggatt
     2401 tccaggatat cacggatccg ctgatgacgg aggccgagcg tcatgatgaa acctgtctgt
     2461 tatatgtggc tgtcacccgg gccctcagga cgctggtact gaatgacctg atgcaccaac
     2521 tggcagaaga acagcagaaa actgaaggtt accgggggac cgggtacgac atctcaggtc
     2581 tggttcagga gaatgggcat gcttgaacgc ctgaaaaggt tatttcctgt gaagacagaa
     2641 tgctctgatg tccggacgga tattcccggg gaaacggtgc cggaggggta ttttacacca
     2701 ttgccggctg atgtactggt atccacaccg ttcaggaaaa ggtgtctgaa acagttatgg
     2761 gaaaacagtt ccatgccggt ggatatgtat cagcgccttt gtcttgatcc tgtgcatcag
     2821 ttgttgtctg tcagtcagaa tctgccggcc acagcagaag gtcactgggg ttacaccggt
     2881 ggttttggtg acctgaccct gcagttcacg acgtatgctg tccggctggc cagaggcaga
     2941 atgtttccac cgggagcgac agcagaagag caggccgcgc aggggagtgt gtggaacatg
     3001 gtggtgttct ggtcggctct gttttatcac ctgccgttgc tggcggaact ggaaggcgaa
     3061 caggccgacg gcagatgctg gcagccgggg atatctgtac cggagggcgt ataccggttc
     3121 cggttcagga agatgccctg ccagggaacg gagggacggc aactggcagc cctgatggcg
     3181 ggacaactgc tgcccggaga ggcgggagcc tggcttgcca ctgtcccggg ggcattacag
     3241 aatcttgccg gagcagtctg gcatcagcat cctgaaatgg cgctgatacg tacagtatta
     3301 caggaagcta tggaggaagt cgtgagtccc ttcgctgcag gagatgtggc cgaagttccg
     3361 gtggctgagg ttgttccggt actgctgcag tcagcagcaa tttcagcaga aagcgtcatt
     3421 cagccgttgc cggttgtacc gtcgccagtg gaggatgccg aaaatgaggg ccggacagcg
     3481 gaacaggagg tggatgaggg gaacgaaact gatgctgata ccggaatgct ccttacgctg
     3541 ttttcggctg ctgatttgtc ggatgaggga gggcagatag cgtcaggcgt gccgccggat
     3601 aaagaaagca gtattgagtt accggaagag agcggatgtg taacagaaaa tcagggaaac
     3661 cgtatcccgg aaacagtgga aaaaacggca gagttttccc tgacagagga tagcagggta
     3721 gagagtgaat ttatcagatg gttaaaagag gggctgaggg aggggtcact ttcggttaac
     3781 agcgttgatt caaatgttca tgttgtttcc ggatatgttt ttctgtgtgc cccggggatt
     3841 ttttatcgtt ttctgaagga gagtggttcc gggcaggatc gtcgttatgt tcagtcgctc
     3901 tttgaacggg caggtatcca taaggtacgt tcaggagagc gtttttttca ggcccgtctt
     3961 tatagtgacg cagaacattc cggacgttac aaaatggttt cgggatatct ggttaagggc
     4021 aggtttctgt acgggaatat tacggttccc ggagacagcc cgcttattgt tttcgggtga
     4081 gtaaaggcga tgatgtgcag aatttgaccg gagagtgcgg gagcacagta aacaagggga
     4141 atatataatg cagaatgacg attccgtcac aaaaatcacc tgggaacaac tgctggaaga
     4201 gtactttttt tcaaagaatc ttcggccgga tacagagtgg agctactgca aagttgtcag
     4261 ggtattcagg gaatttatcg gtgatatttt gccgggggag gtgactgtcc gtgatgttca
     4321 gcgataccgg cgttatgtcc tgaaagagca gaagcgcagg gaggtgacct ggaataacaa
     4381 ggtggcccac ttgcgggcaa ttatgggatt cgggatcaag atgaaactgc tgcctcagac
     4441 agaaaacccg ttggtggaag catcagttgc gagtactgaa aaaaagaaaa agacactgag
     4501 tgacaatcag atgctcaggg tttacctggt tgtgcagagg tttgcagaag cggaagagca
     4561 gaacatggtg aatagtcgtc gacgcaatgc tatataccct gcctggtact ggctgacagt
     4621 tctggatgtt ctgcgttata ccggaatgcg ctttaatcaa ctgaagcata tccgtgtgag
     4681 ggatgtctgt ccggatgaaa gcataattga tcttaagctg gagggaagta aaactcacag
     4741 ggaatggggc gttcctctgg tggctcccct gaaggtatcg atggaaaaac tgctggagcg
     4801 ggcccgtcag tgcggggcag gtgatgatga ctatcttttt gacgtctgtc gtttcagtga
     4861 gccgatctct gccgacagat atcagtactg tcccgttcgg gcgcatcaga ccgtacgttc
     4921 atttttccgg cgactttcgc gagaatgtgg ttttcctgtt tcaccacatc gtttcagaca
     4981 tacgctggca accagactga tgaaatatcc tgaacgtaat ttacatctgg tcaaatccat
     5041 actgggacac aggagtctta caacgaccat ggggtatgtt gatgtctgta tggagtctgc
     5101 agcacagaca ctggaagatg ccctgagttt gtatacagat gagcgcacag tgagcaagat
     5161 ttgcaaaggg gggaataaat cttgacaggc tgaatcagcg atgtaagcat acagtgttaa
     5221 cgagaacagg gcgtctccgc aaaagacacc ctgtttgagg atacgatacc gaactcaatc
     5281 agtgtaacca caacttacac aaacggagac cctttgatgc tgcatcctgt attaaccgcc
     5341 cgtcaaggat ctcatccctt aaccagcgtg ttctggtata tggtgcccgg aggcggaatc
     5401 gaaccaccga cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg
     5461 gggcgcatta aaccgtaatc tgcacatctc gtcaacctaa tttcagtaaa agcgattcaa
     5521 ctgcttaaga ttgcggcaaa tcgcttcttt tctgtgtttc tgtcaggtca atgcaccacc
     5581 ctggcggcag cgggcgaagc gcaggatatc ttccgccagc cgatgtgcag tgtcgacatc
     5641 cgcctggcta cgattcacca gcattcggct taaacagcct tccagcacca gttccatctg
     5701 ctttgctacc atcgccggat cgtcaacttc cagtgtggtt aacagttcgt gggtgaaatc
     5761 gtaggccgcg cttttttgct gatcggccag ttgatgaata gggtggtcag gatcgggata
     5821 aaacgtacag gcagcgataa atagacagcc cggatagcgg ttgtttttaa cgcactccga
     5881 taacgcctga taacgtgcca gcagcttttg ttcggcggtt tgcgtttcgt ccagcatcag
     5941 ctgacgacgc cagacatcta tctgttggct aagataacgc agcgcatcgt agaggattgc
     6001 ctctttgtct ggccagaagc ggcgtagctc gtccagtgga taatccacac gttcagcaac
     6061 catctccagc gtggtgttgg caatcccttg taattctaat aatttcaggg cttctcccag
     6121 tacatcttca cgttgcacgc tattttcctc cgtctttccc actgcaagtg tcgttcacgg
     6181 ttggcgatcg cgcaaatgtg cgctgaaggt ttcagcatcc ataaagcccg tgacgcgtgc
     6241 ttgtggatgc tcctggcctt gtccgtcaaa aaagagaatt gtcggtaggc caaggacatt
     6301 aagatgcttt aacagcgcca catcttgtgc gtcgttggcc gtgacgttgg cctgaagtaa
     6361 gaccgtgtct gctaacgctt tttgcacctg cgggtcgctg aaggtgtatt tctcaaactc
     6421 tttacaggcg acgcaccagt cggcataaag atctaacatc accggtttgc ctttggcttc
     6481 aacgagcgcc tgatttaact catctaccgt tttgatttgt gtaaagttga gatgcgtctg
     6541 agtttgcgcg gtatgcgtcg caccaaatgc ccaatcctga agtgggcgca cgctaaccaa
     6601 tgccgctgcc agcaggatta tttgcaccac gcgcatccag ccgcgtttag cctgtaggct
     6661 ggtgataaag gcccagccaa agaatgcgac acccagcgcc gaccacaagc gtaatcccca
     6721 tatatcacca atcactcgct ccagcaggaa gaccggcagt acgaggatca caaaaccaaa
     6781 cgcggttttg acttgttcca tccacgggcc gcttttcggc agcaagcggt taccaaagac
     6841 ggtaatgagc atcagcggca ggcccatgcc caacgcatag agataaagcg tgccgccgcc
     6901 cagccacatg ttcccgcttt gggcgatata cagcagaatc gcgctaagcg gtgcggtggt
     6961 gcatggtgaa cagatcagtc cggcaatcgc ccccataaca aacacaccgc caggtgagcc
     7021 gccctgttgg cgattgctca tcagcgtgag gcgcgtttgc agcgaggagg ggagttgtag
     7081 agtaaacaag ccaaacattg acatcgccag caaggtaaag acgatagcga ggccaatgag
     7141 cacgtatggg tgctgtagcg ccgcctggaa ctgtaaccct gcggcggcaa ccaccagacc
     7201 cagtgccgtg taagtcagcg ccatcccctg cacataaata aaggtcagca acaatgctct
     7261 ggcagtggaa agccgctgtt taccgcccag cacgatgcca gaaatcagtg ggtacattgg
     7321 cagcacgcat ggcgtaaagg cgataccaat accgatcaac aacgcccaga gcgcggaaaa
     7381 gggcaattgc gcggtgggct gctcttgctg cggaacagac acaggctgtg acgctgcgtt
     7441 gttggtgacc acttcgctta acggaacggt tttggtttct ggcggataac agaaaccggc
     7501 atcagcacag ccctggtagg tgacagttaa cgtcgctccc gcactcgcct ggttgatggt
     7561 tacgggaagc gtcagccgat cgcggtaaat ctcgcttttg ccgtaaaact catcttcatg
     7621 ccagacgcct tgcggcagct gcacgtcggc aattttcgcg tgttccgacg taatgcggat
     7681 ctgtttacgg tagaggtagt aaccgtcttt gatctgccag gtcagattca ggtcatgttg
     7741 gttttgctga aaatcaaaag caaatgcttg atccgcgggg acaaattgtg aacgtcccgg
     7801 cgcgtcgaat aatccggcaa aaacggaagt gctgcaaagt agcaggatca gcgtaaagat
     7861 gcgttgagcc atgagaggta atctgtgtct ccgtgtgtaa caggtaaaac cagaagttcc
     7921 ggggtttgat atggatgatg agacttcagg cattccagca gtgcctgctg gtgagatacg
     7981 gtagttttta aaatcatctg cacttcgtat tcttgctcca gcttaccttc ccagtaatag
     8041 agagaggtag cgccggggat caaggtcgcg caggccgcca gtttttccgc cagcactttg
     8101 gcggctaaat cctgggctgt cgcttcatcc ggtgcggtac atagcaccac gacagacgcg
     8161 gtattcgaac ttttttcatc aagcataaac acctcgcaag aacagatgga accgcaagag
     8221 agaggtcact atacaacgga cgggggaagg atgttagtcg gcgggaaaga aagcggggcg
     8281 tgaacgcccc gcgattggtc attacagcat gaagctaccc agcacgaagc cgaagcaaac
     8341 ggccagggca acacccagag tacccgggat gaagaacgga tggttgaaga cgaatttacc
     8401 gatacgggta gtacccgtgt catccatctg taccgcagca accagcgtcg ggtaggtcgg
     8461 cagaatgaac agaccagaca ccgcagcgaa agaagcaaca gcggtcagcg gagaaacgtt
     8521 cagtgccaga gccatcggca tcagtgcttt tgcggttgca gcctgagagt acagcagagc
     8581 agaagcaaag aagaagatga cggccagcag ccacggatga ccctgaatca cttcaccagc
     8641 ggtatctttg atccagtcga tgttgttgga aacgaaagta tcgcccagcc acgcaacacc
     8701 caggatacaa atacaggcgc tcatacctgc tttgaaggtg ctggagttga ggatgttgtc
     8761 ggtatcgact ttacagataa cggtggtcag agttgcaacg ctgagcatga tgatcaggat
     8821 tgcgttggtg gtgttcatca gcggtttttc aaccagaccc atgcttgggc tgttgatgat
     8881 tgcatagata accacgccaa ctacgcccag caggaacagc cagacggacg tttttgcacc
     8941 ggatttgatt tcaatctgct tttcaccgcg cagttcaacc aggccctctt ccagacgctt
     9001 gcgataaatc ggatcgtcag agagtttgga gttgaacagc atagtgacca ggaaggacat
     9061 caccagaacc gccagcaggg tggacgggat gaccacggag agcagatgga ggtagctgat
     9121 gccatgacct tccatcacgg aagacatgta aaccactgcc gctgagattg gcgatgcggt
     9181 gatcgcaatc tgcgcggata ccactgcagt agacagcgga cggcaaggtt taacgccttg
     9241 ttccttcgca acttcagcga taactggcag tgtcgccaga gagatgttgc cagtaccagc
     9301 aaagatagtc aggaaatagg tcacgatcgg tgcgaggatc gtgatgtatt tcgggttacg
     9361 gcgcagcagc ttttctgtct gatgaaccag atagtccaga ccgccagcaa cctgcatggc
     9421 agaaatagcg gcgataaccg ccatgataat ggaaatgaca tcgaacggga tgttaccggg
     9481 tttaacgcca atagcggcaa gaaccagcac ccccaatccg cctgcaaaac caatacctat
     9541 tccccccaat ctggcgccca agaagatcgc cagcaaaact atgatgagtt ctacaactag
     9601 catattagcc ttccttgttt tttaacaagt tgatattaga ttgttatttt taagttacta
     9661 ctcacaagaa aaaaggcacg tcatctgacg tgccttctct atttgtacta ccctgtacga
     9721 ttactgttcg ctttcatcag tatagcgttt tgctttgtaa gccgggtgca tcagattctg
     9781 tacggagaaa atatcgtcaa gttccgcttc agtcaacaaa ccgcgttcca gaacgacttc
     9841 acgtacactc ttaccggttt cggcacagat tttacccaca atgtcaccgt tgtggtgacc
     9901 gatgaacggg ttcaggtaag taacaatacc gatagagttg taaacgtaac cttcgcacac
     9961 ttctttgtta gcagtgatgc cgttaatgca tttttccagc aggttgtagc aagcgttggt
    10021 cagaatgtga acggattcga acatagcctg gccaatgacc ggctccataa cgttcaactg
    10081 cagctgacct gcttctgctg ccatggtaac agtggtgtcg ttaccgatga ctttgaagca
    10141 tacctggtta accacttccg gaacaaccgg gtttacttta gctggcatga tggaagagcc
    10201 cgcctgcagt tccggcaggt tgatctcgtt caggccggca cgtgggccag aagagagcaa
    10261 gcgcaggtcg ttacagattt tggacatctt cacagccagg cgtttcagcg cgccgtgaac
    10321 cataacataa gcgccgcagt cagaggtcgc ttcgatcagg tcttcagccg gtacgcatgg
    10381 gaagccagtg acttcagcca gttttttcac tgccagcgga gagtactctt tcggcgtgtt
    10441 cagaccagta ccgattgctg ttgcgccaag gttaacttcc agcagcagtt cagcggtacg
    10501 ttggatattt ttcacttctt ctttcagcag gatgctgaaa gcgcggaatt cctgaccgag
    10561 ggtcatcggt actgcgtcct gcagctgggt acgacccatt ttcaggatgt cctggaattc
    10621 gacagcttta cgttcaaagc cttcacgcag ttggttaatc gcatctacca gcttaatcag
    10681 ggaagagtaa actgcgatac ggaaaccggt cgggtaggcg tcgttagtgg actgacattt
    10741 gttaacatgg tcgttcgggt tcaggtactg atattcacct ttctggtgac ccatcagttc
    10801 cagaccgata ttggccagca cttcgttggt gttcatgttt acggaagtac ctgcgccgcc
    10861 ctggtagacg tctaccggga actgatccat gcattttccg ttgttcagga cttcatcaca
    10921 tgcggcaatg atggcattcg ctacactttt aggaatggtt tgcagctctt tgtttgccat
    10981 agctgcggct tttttaacca ttaccatacc gcgaacaaat tcaggaatat cactgatttt
    11041 gttgttgctg atatagaagt tttcaatcgc tctcagagtg tgaacaccat agtaggcatc
    11101 agctggaact tccctggtac ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg
    11161 aaccttcttt ttcaagctgc caatgatttg ctttaaacac acagaatata tgtggtttcg
    11221 aatgtttttc gaccgacgat tatcccctgc atcgaccgaa tacccgagat catatgctgc
    11281 ttgaggattt ctaccgtaat ctggatcact ttaagtgtcg gtttttaccc cttaattatt
    11341 aatttgtgaa atagatcacc gctttgggat tactaccaaa aatagttgcg caaacatctt
    11401 gaaattttgc taatgaccac aatataagct gaacgcgatt cgcaacccat tcaggtagcc
    11461 gggttttacc ggctgctatt acaggagaaa cctttgcgct ggttaccttt tattgccatt
    11521 ttcctttatg tctatattga gatttcaatc tttattcagg ttgcccatgt attgggggta
    11581 ttgctgaccc tcgtgctggt tatattcacg tcagttatcg ggatgtcact ggtacgtaac
    11641 cagggcttta agaatttcgt gctgatgcag caaaaaatgg cggcgggtga aaacccagcg
    11701 gcggagatga ttaaaagtgt ttcgctgatc attgccggtt tgctgctttt attaccgggc
    11761 tttttcaccg acttcctcgg tcttctgctt ttattaccgc cggtgcaaaa gcatctgaca
    11821 gtgaagttga tgccgcattt gcgcttttct cgtatgccag gcggcggttt tagcgccggg
    11881 accggtggcg gtaatacttt tgatggtgag taccagcgaa aggatgatga gcgcgaccgc
    11941 cttgatcata aagacgatcg ccaggattaa tgtcgaaacg ccggattatg tggttatgcc
    12001 attttccggc gtttttcgtt ttggcagaaa cagccataac cccgccagca tgatcagcgc
    12061 atagagactt ttccagccga ccattgccag taacagaacg cataacagcc cgccaaccac
    12121 cgccagtagt cgataacgtc cttgcaataa tttacagcct gccagcatgc ataacagata
    12181 aatcataata aagatgccat tggcataaat aataagagcg tccagattga tctctaaagc
    12241 atgaatcacc aacgtgctca ccacgcagca gccgagcacc gcattgaggg cattattcgg
    12301 gatatggcga gaagagaggc gtgccaggta gtggtcagga ttatgttgcg cctgcgacca
    12361 gaccaggcgg gcgaagctct gtatataaat gttgagactg gcaaagcagg ccagatagcc
    12421 aattacgcag gcaatccata acgctcctac accgaacagt tgcactacaa tttttggaag
    12481 cgatgctgcc gccgccattt gttcaccata ggcgtcgaag tgtaagacga ctaccgtaca
    12541 gccccagtag actaatcctg ccagcagcag accaatcatc aatgcgcgag gaaaatcacg
    12601 ctctggattt ttaaattccg aggtaagatg ggcaaatgct tccagaccga caaaacacca
    12661 gaacatcact gataacgcag caaataaccc ggtaagttcg atatttcctg gcgcggggaa
    12721 ggggatactc gcaggtttga tatcgcccgc ccaccagata gcgacaatca gtgcgacgat
    12781 aagtccggca ataacggttt gtagattagc actggaactg gcaccgcgag taccgatata
    12841 ccacaccagc gccagcgtac cgagttctgc caacaacagt tgccagctat gccagccaaa
    12901 cattgcctgg ccgaacccgg cggcaatttg tagcgcggca ggcaaaccca cgggaatgac
    12961 cgataaaaac aaccagccgg tgactcgctc aagtcgcgaa ccaaacgcca taccgacgaa
    13021 gtgcgcgaca ccgcctgcgc tgggatagtg gcgacccaga atcgcaaaca caatcgcaat
    13081 cgggaacact aagataatca aaacgggcca cgcccacagg ctgttattgc ccgctaccag
    13141 cgcagctaac gcaggaacgg caaacacgcc agtgcctaat aatgacgtcg atagcaggcc
    13201 aatgccctgg gccagcccca gttcttgttt gagtccactc atgggttgat atccgattgc
    13261 gcccaaattt tgggcaactg cgtagatttt cgatggtagc acaatcagat tcgcttatga
    13321 cggcgatgaa gaaattgcga tgaaatgtga ggtgaatcag ggttttcacc cgattttgtg
    13381 ctgatcagaa ttttttttct ttttccccct tgaaggggcg aatcctcatc cccatttctc
    13441 tggtcaccag ccgggaaacc acgtaagctc cggcgtcacc cataacagat acggactttc
    13501 tcaaaggaga gttatcaatg aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta
    13561 aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca gcggctaaat
    13621 ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat ggcgaagtga
    13681 agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat
    13741 ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt ctggcaattg
    13801 ttgaagcgta atcctcgcac gacactgaac atacgaattt aaggaataaa gataatggca
    13861 gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta
    13921 ctggcagatg cagtgaaagt taccctcggt ccgaaaggcc gtaacgtagt tctggataaa
    13981 tctttcggtg caccgaccat caccaaagat ggtgtttccg ttgctcgtga aatcgaactg
    14041 gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc taaagcgaac
    14101 gacgctgcag gcgacggtac caccactgca actgtactgg ctcaggctat catcactgag
    14161 ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa
    14221 gccgttaccg ctgcagttga agaactgaaa gcgctgtccg taccgtgctc tgactctaaa
    14281 gcgattgctc aggttggtac catctccgct aactccgacg aaaccgtagg taaactgatc
    14341 gctgaagcga tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt
    14401 ctgcaggacg aactggacgt ggttgaaggt atgcagttcg accgtggcta cctgtctcct
    14461 tacttcatca acaagccgga aactggcgca gtagaactgg aaagcccgtt catcctgctg
    14521 gctgacaaga aaatctccaa catccgcgaa atgctgccgg ttctggaagc cgttgccaaa
    14581 gcaggtaaac cgctgctgat catcgctgaa gatgttgaag gcgaagcgct ggcaactctg
    14641 gttgttaaca ccatgcgtgg catcgtgaaa gttgctgcgg ttaaagctcc gggcttcggc
    14701 gatcgtcgta aagctatgct gcaggatatc gcaaccctga ctggcggtac cgtaatctct
    14761 gaagagatcg gtatggagct ggaaaaagca accctggaag acctgggtca ggctaaacgt
    14821 gttgtgatca acaaagacac caccactatc atcgatggcg tgggtgaaga agctgcaatc
    14881 cagggccgtg ttgctcagat ccgtcagcag attgaagaag caacttctga ctacgaccgt
    14941 gaaaaactgc aggaacgcgt agcgaaactg gcaggcggcg ttgcagttat caaagtaggt
    15001 gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg ttgaagacgc cctgcacgcg
    15061 acccgtgctg cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta
    15121 gcgtctaaac tggctgacct gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt
    15181 gcactgcgtg caatggaagc tccgctgcgt cagatcgtcc tgaactgcgg cgaagaaccg
    15241 tctgttgttg ctaacaccgt taaaggcggc gacggcaact acggttacaa cgcagcaacc
    15301 gaagaatacg gcaacatgat cgacatgggt atcctggacc caaccaaagt aacccgttct
    15361 gctctgcagt acgcggcttc tgtggctggc ctgatgatca ccaccgagtg catggttacc
    15421 gacctgccga aaaatgatgc agctgactta ggcgctgctg gcggtatggg cggcatgggt
    15481 ggcatgggcg gcatgatgta attgccctgc acctcgcaga aataaacaaa cccccgggca
    15541 gaaatgtctg ggggtttttc ttttggtcat ctttctagta taagattcag acacggacga
    15601 cgcgagtggc gtccagctca ttgattatgg ggaataacat gcacgtaaaa tacttagcag
    15661 ggattgtcgg tgccgcgcta ctgatggcgg gttgtagctc cagcaacgaa ttgagtgctg
    15721 ccggtcagag tgtacgcatt gtggacgagc agccaggcgc agagtgccag ctgattggta
    15781 ctgcgacagg taagcaaagc aactggcttt ccgggcaaca cggagaagag ggcggttcta
    15841 tgcgcggcgc agcaaacgat ctgcgcaacc aggcggctgc aatgggcggt aacgtgattt
    15901 atggcatcag cagcccgtcg cagggaatgt tgtccagttt tgtcccgacg gatagccaga
    15961 ttatcggtca ggtttataag tgcccgaact gatgtgatcc agaggctggg tgacaaacgt
    16021 atcattgcct gatgcgcttc gcttatcagg cctacagcag gcattgcaac tgattgaatg
    16081 cttgagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgac caacgatcac
    16141 gttatcagtc atttcaggct ggatggctcc agcctcgcat tagcaaagat gagattattt
    16201 cgcctgtggt gcagttttgc tggtggattg aacacgtaag tgatgcagcg cgcgtaagca
    16261 ttgatcctgg gttagcggtt gggcgttatt cgccgctacg gtgcgaacat agacctgaca
    16321 tggcacaccc gcgagttttt tcagtcgttg gctgaaatca agcattcggc gagtgatcgc
    16381 ctctatttct ttacccggcg ttgcttcacg cgttttcacc actgcgccgc taaattgttc
    16441 tttgccactt tcatctttaa agcggtaaag caccgccaga taatggtcga acaggttatg
    16501 tttccattcc ggttgggaat aggtgtcata ttccagattg ccattttcct ggcagtcaac
    16561 gtcatataac ggcaggaaat ctaacagtga ggtaatacgt aatgccagtt cacgcatttc
    16621 cgcattatta attgcatgaa tgattgctcg cgccagcatt tcaatctgca actcattgac
    16681 gactaactca catttgctac cgtcatgtag cgttaatacg aaggtaagat tttcgccttg
    16741 attatcggta agttccagcg tgttaacccg acgattaata tcggcatttt taagctcatc
    16801 aaccagaatt tctgggatgt tttctgccat tttcttaatg actttatcgc gagcttgctc
    16861 atactgaagg cgtgcagcag catccagttt atgcttttga tgcatacgac tttccagcgc
    16921 aatcagcagg tcgcgcagtt ccatgacaga cataaagaac aacgattctt tattgcgagg
    16981 ttctttaatt ttcagcgcca gtgcaatgaa attattactc ttgcgaataa caccggtatt
    17041 aacacccttg atgcttatgg ccatgtaggt tctccctaac catttctcaa ttaaataatt
    17101 aattttaatt tataagccag ataaatgggc ttggtagtaa tagttgttaa aataacataa
    17161 atagccgtac tcactctata taaacagaaa agaaaggtta attattggtg ttggctatat
    17221 aaagagtaac aacagcaatg tatatgaata attaaaggtt atgcaacggg caaagattaa
    17281 atttcccagt ttaatgacag gttgctgtac ttgcgtttgt tgctcttgcc ggatgcggcg
    17341 taaacgcctt atccggccta cagttcattg caaatccatt caatttcaat gcacaatgta
    17401 ggcctgataa gcgtagcgca tcaggctaat gtttaacttc cctgtttaat cagttactgc
    17461 tggcgtagct ggagatccag cggcgttttg ctgggttcgc cgccgatttc tcgcgccagt
    17521 ttcggcacca gataacccga caccagtgtc agcaactcac gcataatctg ccgtgcttcg
    17581 tcatcactca ccataaaatg cgccgcgccc tgtactttat cgagcacatg caggtaatac
    17641 ggcattacgc cggcatcgaa caacgcatta ctcaggtttg ccagcgtttt tgcgttatcg
    17701 ttcacaccac gtaacagaac gctctggtta agcagggtga caccgacacg gcgcaattta
    17761 gccatcgcct gacggaacgt ttcatctacc tcattggcat gattgatgtg attcaccagc
    17821 aagatttgca gcgtagaacg ggcaaagcgt tcaaccagcg cctcggtgat acgtgccggg
    17881 atcacaatcg gcagacggct gtgaatccgc agacgtttga tatgcgggat ggcttccagt
    17941 tgtgtgagca accagtccag ctcgtgatct ttcgccatca gcggatcgcc gccggagaaa
    18001 atcatctcgt ccagttccgg atgagcagca acatactcaa gagcagtctg ccagttacgc
    18061 ttgttgccct gattttcggc ataggggaag tggcgacgga agcaataacg gcaatttacc
    18121 gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt atttatgcaa caaaccaggc
    18181 actacgctgt gctgttcttc cagtgggtcg gtggagaatc cgggcgcgac gacaaactca
    18241 tcttgcgagg taagtacctg acgcaaaaga ggatcgtccg gattgccttt ctccatgcga
    18301 tcgataaatg agcggggcac acgcagggca aacagctttt tggcgctgcg tccggccaac
    18361 agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt catcaggatc ggtcacaaca
    18421 tcggcaagtt gcgttaacca atcttctctg gatggggtat ttagggttac aatatgcgcc
    18481 attttgtggc ttagctacca attaacaaat ttcagagggc cttatggcaa cgtactatag
    18541 caacgatttt cgtgctggtc ttaaaatcat gttagacggc gaaccttacg cggttgaagc
    18601 gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac tgcgtcgtct
    18661 gctgaccggt actcgcgtag aaaaaacctt caaatctact gattccgctg aaggcgctga
    18721 tgttgtcgat atgaacctga cttacctgta caacgacggt gagttctggc acttcatgaa
    18781 caacgaaact ttcgagcagc tgtctgctga tgcaaaagca attggcgaca acgctaaatg
    18841 gctgctggat caggcagagt gtatcgtaac tctgtggaat ggtcagccga tctccgttac
    18901 tccgccgaac ttcgttgaac tggaaatcgt tgataccgat ccgggcctga aaggtgatac
    18961 cgcaggtact ggcggcaaac cggctaccct gtctactggc gctgtggtta aagttccgct
    19021 gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc tctggtgaat acgtctctcg
    19081 cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac catcacttat tcaggtcaga
    19141 gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc acgctgctca cgggctgtaa
    19201 caccgctcgc ggtttcggcg aagacatcaa acatctcggc aactccatct cccgcgctgc
    19261 cagctaattt ttcttctctt ccgaaaaatc atcagatttc catcattttt ggcgatgttg
    19321 tctattatta atttgctata ggcaaacata aataacatta cctaaaagga agacgttatg
    19381 gtgaagaaga caattgcagc gatcttttct gttctggtgc tttcaacagt attaactgcc
    19441 tgcaacacca cgcgtggcgt tggtgaagac atttctgatg gcggtaacgc gatttctggt
    19501 gcagcaacga aagcgcagca ataagcaata acggtacgac agttatgtcg tgccgtttgt
    19561 ttttttgcga tagtcacaaa ggtaatagtt gaaattcccc tgccacctgg caaaatatcc
    19621 gttcaaccat cagctttgca ggacgacctg caaacgcctc ttttcaccgg ggacggcccc
    19681 aattctccgg agcctgatat gtcctggatt atcttagtta ttgctggtct gctggaagtg
    19741 gtatgggccg ttggcctgaa atatacccac ggctttagcc gtttgacgcc gagtgttatt
    19801 accgtgacgg cgatgatagt cagtatggcg ctacttgcct gggcgatgaa atcgttacca
    19861 gtagggacgg cttatgccgt gtggacgggt attggcgcag tcggcgcggc tatcaccggc
    19921 attgtgctgc tcggtgagtc cgctaacccg atgcgcctgg cgagtctggc gttaatcgta
    19981 ttggggatta ttggtctgaa actcagcact cactaactac cgggctgctg tacccaaata
    20041 aacttactga catcaaaccc ttcccgggtc gcgactgcca gcatctcctg tttcacttcg
    20101 tcagaaatgg ttggcgtgcg ggagagtatc cacaggtagt cgcggtccgg gccgcaaacc
    20161 agcgcatggc ggtattcccg atcgagtgca ataacgttat aaccgccata gaaaggacca
    20221 aagaatgaca ctttcagcgc agcgcgagtt ggtgcgccgg taaagtacgc tttcccttca
    20281 ctctgctgcc acattcctct gtcagggtta tagcctttat taatgacatt caggccgccg
    20341 tcatcacgca ggctgtatgt tgcggtgact ttttccagtc cacgttcaaa acggtgatca
    20401 aaacgggcaa tctcatacca ggtaccgaga taacgtttgg cgtcgaaatt attcactact
    20461 gttacgccac gcggcggcgt aggagaactg caggcaacga ccagaaatgc agctgtcgct
    20521 gcggcaacga gagggagcag gcgcataaat gtttccttac tggttttttt ctaagtgtag
    20581 atgacagcaa ggaaaagcgc agaaaaggtc cgaaaattcg gacccgatga aattttactg
    20641 tagagcgttg agaatctgcc aggcggcggc gactctcgct ggattgggat agtttttgtt
    20701 agccagcatc actataccca gctctttttc tggaataaac gcgacatagc taccaaatcc
    20761 gccggttgcc cctgttttat gtacccatga tgcgcgtact gcaggagttg ggggcgtaat
    20821 cgcttttacg gggtgtgctg ccagtgcaat tttattgcca ctgccgttaa tgatgctgtc
    20881 aggatttacc ggccagtcca gcatttccca gcccaggccc tgatacatat cgccggtttg
    20941 ccagtagcga gattgtgcca gttgtatccc ttgctgaagt gttttgtcgt tgatatcacg
    21001 gggattcata ttgcttcgta cccagcaggc catatcttca atggtcgact tcacaccata
    21061 agttttagca tctaacgccc ctggcgaaac atgaactgcc ttgccctcgc gatatcccca
    21121 ggcgtaattc ttttcttctg cgggcggtac attaatccac gtatggttga gtttgagtgg
    21181 ctggaagaca cgtgtttgca tcgcctgctc aaaactcaaa ccagacggct tcacagccag
    21241 tgcgccgaac aaaccgatac tggagttggc atacagacgt tgtgttcctg gcgcccatgc
    21301 aggctgccag ttttgataga agcgcagcaa gtcgcttgag gatttcacct catccggcac
    21361 ctgcaatggc aggccgccag cagtgtaggt tgcgagatgt aatagtgtga tcccattcca
    21421 ctgtttagcg gtaagttcag gccagtattt tgttgtggga tcgcttaact tgatttcccc
    21481 tcgagcaata gcgtcgccac caagcacgcc cgtaaatgtt ttgctgaccg aacctaactc
    21541 aaacaacgtt tgctgtgtga cgggctgctt tttggcgatg tccgcatagc cccaggtaaa
    21601 gtaataaggt ttaccctgat aaattaccgc caccgccata ccggggatct tttgttgctc
    21661 tataagcggg gtaattgtgc gatgcacaat atcgttgatt tgttgagggg cagcaaatgt
    21721 ggagcaagag gcggtaatta ataaggcgca gagcgtcgtt ttgaacataa ggtctggttt
    21781 ccatacaaaa cggcccgcca taggcgggcc ggatttacat tggcgatacg ttagattgta
    21841 acgataccaa tcagcgtgac aactgtcagg atagcagcca gaccgtagaa aacccatttg
    21901 cccgcaggca cgtgaatttt cagatcgtgc atcgcgtggt gcatacggtg taaaccacac
    21961 cacagcggca gaacgatcat caggaacagg aatacgcgac caatgaagct ctgcgcgaac
    22021 gccagaacgc gctcgtagct cagcgcatca cccggaaaca gccccagtgg cagcagaata
    22081 cccaccagca ggatcatcac cggcgcaata attgcgctcc acataccacc ggccccgaag
    22141 aggccccaga ataccggttc gtcagaacgc tttggatttg gattaatcat ctcaggctcc
    22201 ttaccagtac agggcaacaa acaggattac gatggtggca accacagtta ccgcccagag
    22261 acttttgata attggctctg gtcccatttt ttcgtctttt acaatgatat tggccgcttt
    22321 cggtgccagt tcaaaccagg ttttggtgtg cagcagagct gccgccagag tgatcaggtt
    22381 aatgatcacg ataaccgggt tttgtaaaaa gtcgacgaat cccgcccagg cttccgggcc
    22441 atttttcagg gcaaacagcc cgaaaatcag ttcaatgctg aaccacacag ccggaaccgc
    22501 cgtgccttcg cgcagcatgt aaaagcgata aaacggcaat tttttccacc aggtggacgt
    22561 cattggccgt acatacggtt tacgtttagt cgtcatgttg cactccttag cgtggtttca
    22621 gggtcgcgat aagaaagtct ttcgaacttt ctactttgcc ctgctgaatg gccgcagccg
    22681 gatcgacgtg tttcgggcag acttcggagc aatagcccac gaaagtacag ctccatacgc
    22741 cgttctggct gttcaactgc gccatacgct ccttcttacc gtggtcgcgg ctatcttcgt
    22801 tataacgatg cgccagcgta atggcagccg gaccgatgaa ctctgggttc aggccaaact
    22861 gcgggcacgc ggcgtagcac agaccacagt tgatgcaacc ggagaactgg tgatactttg
    22921 ccatctgcgc cggggtctgg atgttagtac cctgatccgc ggtgcgggag ttgccgatga
    22981 tgtacggttt gatcgcttcc agactttcga tgaagtgggt catatcgacc accagatcgc
    23041 gttcaatcgg gaagttagct aacgcttcaa ccttcatacc gtcggtgtaa tcacgcagga
    23101 aggttttaca tgccagtttt ggcacgttgt taaccatcat gccgcaggaa ccacaaatcg
    23161 ccatacggca ggaccagcgg taactcaggt ccggtgccag gttgtctttg atgtagccca
    23221 gcgcatccag taatgaggta gttgcgtcat aaggcacttc atagaatgcg ctatgcggtg
    23281 cggtatcgac ttccgggtta tagcgcacca cctcaatttt caggtttttc atctcagcca
    23341 ttcgccttct ccttcttatt ggctgcttcc gccttatcgg ctgcatccgc ttcgccaccg
    23401 taaacgcgtt tagctggcgg cagcgtagta atcttcacgt cgctgtactc caggcgagtc
    23461 gtgccatcag catcgcggaa ggcgagggtg tgtttgagga agttgacgtc gtcacgctcg
    23521 gtgcaacctt cgtccagacg ctggtgtgcg ccgcgggact ctttacgtgc cattgcggag
    23581 tgcgccatac attcagcaac gttcagaccg tggcccagtt caatggtgta gagcaggtcg
    23641 gtgttgaaca cgctggaggt gtcggtgatg cgcacgcgct tgaagcgttc ctgcagctct
    23701 gccagcttgt cgatggtttt ctgcatcagt tccggcgtac ggtagatacc gcaaccttct
    23761 tccattgcca ggcccatttc gtcgcggatc ttcgcccagt tttcgccgcc atcctggtta
    23821 accagatctt tcagacgttg ttcaacgcca gctgcctgcg cttcaattgc cgcttcgttg
    23881 ccattaccgg cagttgctgc acgctctgtc gcttgttcac cggccagacg gccgaagacc
    23941 accagttccg ccagggagtt agaacccaga cggtttgcac cgtgcagacc aacagaggaa
    24001 cattcaccca cggcgaacag acctttaatg cgggtttcac agttctgatc ggtttcgata
    24061 ccgcccatgg tgtagtgtgc ggtcggacgt accggaatcg gttctttaac cggatcgacg
    24121 ccaacgtacg ctttcgccag ttcgcagatg aacggcagac gttcatgcag ttttttctcg
    24181 ccgaggtgac gcaggtcgag ataaaccaca tcgccacgcg gcgtggagat ggtgttgcct
    24241 ttacgccatt cgtgccagaa ggcctgagaa actttgtcgc gtggacccag ttccatatat
    24301 ttgtttttcg gctcgcccag cggagtttcc gggcccatgc cgtaatcttg cagataacgg
    24361 tagccatttt tgttgaccag aataccgcct tcaccgcggc agccttcggt catcaggata
    24421 ccggaacctg gcagaccggt tgggtgatac tgaacgaatt ccatgtcacg cagcggaacg
    24481 ccgtggctta gcgccatacc cataccgtca ccggtaacga tgccgccgtt ggtgttgtaa
    24541 cgataaacgc gacccgcacc gccagtagcc ataacgaccg cgttagcacg gatctgcacc
    24601 agcgtgcctt ccatcatgtt cattgctacc aggccgcgaa catgaccatc atcaaccaga
    24661 atatccagca cgaaatgttc gtcaaaacgc tggatctgcg ggaattgcag agaggtctgg
    24721 aacagcgtgt gcagcatatg gaagccggtc ttatcggcgg cgaaccaagt gcgctcgatt
    24781 ttcatgccgc cgaagcgacg tacgttgacg ctaccatccg ggcgacggct ccatgggcac
    24841 ccccacagtt ccagttgggt catttcggtt gggcagtggt ggacgaaata atccacgaca
    24901 tcctgctcac acaaccagtc gccacccgct actgtatcgt gaaagtgata ttcgaagctg
    24961 tcatgatcct gcgcgacagc ggcggagccc ccttctgcag caacggtatg gctacgcatc
    25021 gggtatactt ttgagattag tgcgattttt gcatttggat ttgcctgcgc ggcagcaatt
    25081 gcagcacgta atcccgcgcc accggcgcct acaatggcaa gatcggcttg aaaggtttgc
    25141 acgacattcc tccagattgt ttttatccca cagccccgta cttcagggta agtgcctgaa
    25201 agttacggtc tgcgaacgct attccactgc tcctttatag gtacaacagt atagtctgat
    25261 ggataagtct gaaatttgac gagatcgatt tttttagtgc gcaagagggc taaattatca
    25321 ctgaagatga ttaatttaat tactaaacca tcagatcgtg ctttttttag tcactgccca
    25381 ccgctgtttg attcctgcgt aaaatttgtc tcgccgtcgc gttgcgagta gacttcgtgc
    25441 ccttgtcaaa aactggagat ttaactatga gcgaaacggc atcctggcag ccgagcgcat
    25501 ccattcctaa cttattaaaa cgcgcggcga ttatggcgga gatccgtcgt ttctttgccg
    25561 atcgtggagt gctggaggtg gagacgcctt gtatgagcca ggcgacggta accgatattc
    25621 atttggtccc gtttgagaca cgtttcgttg gccccgggca ttcgcagggg atgaatctct
    25681 ggttaatgac cagcccggaa taccatatga aacgcctgct ggttgccggt tgtgggccgg
    25741 tattccagct gtgccgcagc ttccgtaatg aagagatggg gcgttatcac aaccctgagt
    25801 tcactatgct ggagtggtat cgaccgcact atgatatgta ccggttgatg aacgaggtgg
    25861 acgatctctt acaacaggtg ctggactgcc cggcagcaga aagcctttct tatcaacaag
    25921 ctttcttgcg ttatctggaa attgacccgc tctctgccga caaaacgcaa ctgcgggaag
    25981 tcgcagcgaa actggatttg agcaatgttg ctgataccga agaagaccgc gacacgctgc
    26041 tacaattgct gtttaccttt ggcgtagagc caaatattgg caaagaaaaa ccgacctttg
    26101 tgtaccactt tccagccagc caggcatcac tggcgcaaat cagtaccgaa gatcatcggg
    26161 tcgctgaacg ctttgaggtt tattataaag gtattgagct ggcgaatggt ttccatgaat
    26221 tgacggatgc ccgtgagcag caacaacgct ttgaacaaga taaccgtaag cgcgcggcgc
    26281 gcggtttgcc gcagcacccc attgaccaga atctgattga agccttgaaa gtcggtatgc
    26341 ctgactgttc cggcgtggca ttaggtgttg atcgtctggt gatgttggcg ctgggcgcgg
    26401 agacactggc tgaagtcatc gcctttagcg ttgaccgggc ataattctga aaaattactg
    26461 aattaacagc gaagaatggc gtgataagcg ccattctgta gcatatgttt tcatttttcc
    26521 gttaaaggcc acaatttaag actggatatt tgatatcatc caggtatcaa ttctgtattg
    26581 gtttttacgc tgcctgctca atattgcgca gtgcagttca gtgggcacgt gttatacacg
    26641 cgctgaaatg aaggatggtt tcatgcctca cacgataaaa aagatgagtc tgataggact
    26701 catattgatg atctttactt ccgtatttgg atttgccaat agcccatcgg cttattactt
    26761 aatgggttat agtgcgattc ccttttatat attttctgca ttgttattct ttattccatt
    26821 cgccttaatg atggctgaaa tgggagctgc ttatcgcaaa gaagaaggcg gtatctattc
    26881 ctggatgaat aatagtgtcg gaccacgttt tgccttcatt ggtacgttta tgtggttttc
    26941 ctcttatatc atctggatgg tgagtacctc cgcgaaagtt tgggtaccgt tctcaacatt
    27001 cctctatggt agcgacatga cccagcactg gcgtattgct ggactggagc ctacgcaggt
    27061 ggttggtctg ctggcagtgg catggatgat tctggtcacc gtcgttgctt caaaggggat
    27121 taataaaatt gcccgcatta ctgcggtggg cggtattgca gtaatgtgtc tgaatttagt
    27181 attgctgtta gtaagcatta ctattttgtt attaaatggt gggcatttcg cgcaggatat
    27241 taatttcctt gcatcaccga acccaggtta tcagtccggt ctggcaatgc tatcgtttgt
    27301 ggtatttgct atttttgcct atggcggaat tgaagcggtt ggtggtctgg tcgataaaac
    27361 ggaaaatcca gaaaagaact ttgccaaagg tattgttttt gccgctattg ttatttcaat
    27421 cggttattcg ctggcaatat ttttatgggg tgtcagcaca aactggcagc aggtattaag
    27481 taatggttcc gttaacctcg gcaatattac ctatgtgctg atgaagagcc tcggggtgac
    27541 gctgggtaac gcactgcatt tgtcacctga agcgtcattg tcgctgggtg tatggtttgc
    27601 gcgtattacc ggactttcga tgttcctcgc ttataccggg gcgttcttta cgctttgcta
    27661 ttcaccgctg aaagccatca tccaggggac gccgaaagcg ttgtggccgg aaccgatgac
    27721 gcgcctgaat gcgatgggga tgccttctat cgccatgtgg atgcagtgcg ggttggttac
    27781 tgtcttcatc ctgctggttt cgtttggtgg cggtaccgca tcggcgttct ttaacaagct
    27841 gacgctgatg gcgaacgtgt ctatgacgct tccttacctg ttcctcgcgc tggctttccc
    27901 gttctttaaa gcacgtcagg atctcgacag accgtttgtg attttcaaaa cgcatttgtc
    27961 ggcaatgatt gcgacagtgg ttgtcgtact ggtggtgaca tttgcgaacg tcttcaccat
    28021 cattcaacct gtggttgaag ccggagactg ggacagcaca ttgtggatga ttggcggccc
    28081 tgtcttcttc tcgctgttag cgatggcgat ttaccagaac tattgcagca gagtggcaaa
    28141 aaatccgcag tgggcggtgg aataacccga tgccctatcg tctgcctgac gatagggcgc
    28201 aatgacagga aatgttatgg atgacaccag ccgggacccg gcaataaccg aggatgaaat
    28261 cagggcgctg caattttctg ccggggatgt agcggaaata gagcagacta ttctctcttt
    28321 tgtcgatgcc tgccacaccc gtaaagtggc gatggtggtg ggtagcacca ttaacaccct
    28381 caaagatcgc gatggtaaac ggtggggaaa cctgctggat atttattgcg cttatcttat
    28441 ccgatgtctg gtttttcgcg gggaattagt gggatacggc gatttgtttc atatgcgcta
    28501 tagcgaaatt aagcgaccgg ttaccttatg agtgcgcgca tgtttgtctt gtgctgcatc
    28561 tggttcattg tggcgttcct ctggataacc attacctccg cgctggacaa agagtggatg
    28621 attgatggac gcgggattaa caacgtctgc gatgtgttga tgtatctcga agaagacgat
    28681 atccgcgatg tcggtgtgat aatgacgtta ccgctctttt tcccatttct ctggttcgcg
    28741 ctgtggcgaa aaaaacgcgg ctggtttatg tacgccaccg cgctggctat tttcggctac
    28801 tggctgtggc agttttttct gcgctatcag ttttgtttgt gagccggatg gggtcatccg
    28861 gccacaaaga ccttacaaac ttcccgcctg acgacctttg cccgcagaag ttaatgttct
    28921 ccccgtttgc ttaccattca ggctttccag acgcatctgg aacggtggga atggcatgtc
    28981 gataccgtgg gcgtggaagc ctgccagaat taactggtgg atctcatggc gcagcggcat
    29041 acggtgaccc atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc cctgttgcag
    29101 atccaccagg aagacttccg gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc
    29161 ggtgagcagg atttccgtca cttcttcact gttggcatcg gcaggggccg gaatcgtcaa
    29221 caccacgcgc gtaaccgagt cagagagcga ccagttgata aactgctcgg taataaacgc
    29281 cttgttcggc acgattatct ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt
    29341 aattttcgtc acgctaccgg tgagatcgcg aattgtcacc gtatcgccaa tgcgaatcgg
    29401 tttttcgaac aggataatca gaccagagat aaagttggcg aaaatttcct gcaaaccaaa
    29461 accgagacca acaccgagcg cggcgaccag ccactgcaat ttcgaccact caataccaat
    29521 catcgagaag ccgaccagcc cgccaatcag catcagcaga tatttggtga tggtggtgat
    29581 ggcgtagccc gtacccggcg ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc
    29641 gggcaggttg cgcaccagct gcgtggtgat gataaacacc agaatggcaa tcagcaccgc
    29701 accgagggta attggctcca gactttctac gccctgtacc gtggaggtga catcccacag
    29761 cgaaatattt tcgaggaagc cgaaagcgga atggatttct gaccacagca caatgacaga
    29821 aagcagggcg atcaacatca aaattgagcg caccagccgc aaggattgcg cactgatggc
    29881 atcgagatcg acttcgcttt catcgacttc aattgcccct tccgggctac tgtgatgatg
    29941 cgcttcctct tcgccacgcg cgcgttgcgc taacatctct gcccggcgat gcttcgcccg
    30001 gtcaaacgcc agcctgcgac gctggatcag catccagcgg cggataacgt gataaaccac
    30061 cagtagcagg aaccagatgg caaccgaggt ttcaagcctc gctaacagtg cctgcgccgt
    30121 tgccagataa cccaccgctg acgccagaat ggcaaccagt ggtgcgccaa tcatcatgtt
    30181 ccacagcata tggttggtaa tgttgtcgcc gctgccctct ttgttgaggt acagcgggat
    30241 cccggccttt ttcaggctga gggtgaccac cgccagcgca ccgcaaatga ggataaagca
    30301 aagccgtccc agcgaaccgg agaattcacg gtcgtcgagg ttatcgaaca tcatcagcgc
    30361 cataatcagc ggcacaataa gcccgatgct catcaggtag tagcgcatcc cacgggaaac
    30421 acgttcgcgc ggccagccaa aatgagcgat aaacaagccg ttcgggcggg caaaggtggc
    30481 gcaaatcatc actacccaca gcagcggcac ggtggccgtt acgccatcgc caatcgcgac
    30541 cgccagcgga taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg
    30601 cagcggtgac gcgacgagaa tcgaccagaa aagagtgcgc aacgtcagcc agaagtgatc
    30661 ctgagtcact ttgccgactt tcgccgccga acgttcaaga aaacgggtga aatagcggcg
    30721 cgagtaaata ctgcaaccga ccagaatcaa cgcgccaaac agcggcaaaa tcgtctcttt
    30781 gctggtcagc atcatcacac tggctttgcc caactgactg aaggtgtcca gcgaaatgag
    30841 acgacgcaga tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga
    30901 ggtccagaac agatagcggt gcgttgcttc gttcacctct ttcagcgcat cctccagttg
    30961 cccgttggag actttcagct tggtcagttc cagtagtagc gtgtcgccac cctgcaacaa
    31021 tgagttcagc aactcacgct gagtgcgcag ttgtgcttcc agaatacggt tttgctcggc
    31081 ggtcagcggc tgaccgtcgg cctgatggat ttgccgtagc agcggctgtt tattaagcag
    31141 atcctcataa cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa gctgttgtgg
    31201 ttttggcatt tctggtagcc gtgccacctg cgcccgcagc gcttcaccga gcaggttgga
    31261 cgatcccagc cattgcgact gttcacgcag cgtattcaac gcctgccgga cctgtaacgt
    31321 ctggctggca gcctgacgct gttgcgaggc aacgagatcc atccgctgcg cctgttgatt
    31381 cagtgccgcc gatagttcgc ggttaatttt gaattgcgcg acgatatctt tcggcaaatc
    31441 ggcactgttt tctgccagta attcggtact ttccagcgcc cgctccgcct caagctgacg
    31501 ttggctattc aactgattac gtaaggcctg caaatacgca tccagttgct ggctctcttt
    31561 ttccgccagc tctgagcgca agcgcgctaa ttcctgacgg ttattggcag acagctgcgc
    31621 cagttccagt tcatcaacga gcgctttcag gcgtgcggag tcagactgca acgcgaaatt
    31681 ttgtgcctga ttgagcggag tattgccggt aagcgttccc aggcggcgct cgatctcatt
    31741 taactgacgg cgggcatcgg tttgctgttg cggcagttga ttcagtgaat cggcaatctc
    31801 gcgggcgcgc tcctgctctt gctgggcctg acggctctta tccagcaact gactgctgac
    31861 ctggagaatt tcctgattca gcgcatcggt agacattccc ggcgacacgc tgcgcggctc
    31921 gtcacgcatg ttgtttaatt gtgcgcgcag agtagcggag agtttcgggt aattatcgat
    31981 aacttgctga tattgtttga tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa
    32041 ggcagactgg agtgcctcta tgacttccgg ctgcgcgggt ttcgccgctt ttgcctgctc
    32101 cagttcctgg gtgatttgtt tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag
    32161 gcaccaggcc atcagaaaag tgataatcag gcgcacgtca gcgtttcctt tgatggatta
    32221 gacctggtct tttttgtcgt caaccaatgg gctggcgtcg tgttctgctt cgatctcttc
    32281 agcaggaagc ggggcaggtt cagcgtctgg cgtaacaaag gtttcggtag atactgccag
    32341 cggctggcca attttcgtga cagacaggct ttccagttgc tcaaccagat tcactttacc
    32401 cggtgcaaac aggttgataa cggtggaacc gagtttaaag cgccccattt cctggccttt
    32461 aagcagtgcc acagaaccgt cgttttcccc ggcaggccag gtccagcgct tgatgatacc
    32521 ttcgcgcggc ggcgtaatgg tgcccgccca gaccgtctca atgctgccaa caatcgtcgc
    32581 tccgaccaga atctgcgcca ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc
    32641 gttacgggca aacagattcg gcacgttctg agcggtgaga tggttaacgg agaagagatc
    32701 gcccggcacg tagatcatct cacgcagaat accgttgcac ggcatgtgta cgcggtggta
    32761 gtcacgcggg gagaggtaag tggtcacaaa cgtaccgttg cggaacaggt tcgccatcag
    32821 atagttgcct gccagcaagg cttcgaggct gtagttgtgg cctttggctt gcaggatttt
    32881 atcttcttcg attttaccca actggctgat aacgccatcg gcaggcatga ccaggacatt
    32941 cggatcggta tcgattgggc gtacttcgtc acgcagcgga cggacaaaga attcgttaaa
    33001 ggtgcggtag ctggcggtgt ccggcttttg cgcctctttc atgtcgacct tgtagtattt
    33061 aacgaacaga tcgataacca gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc
    33121 cgccaggcga gtaagccata gtttcggcag aatgtactgt agcgaaagtt taaatgaatt
    33181 taacaaggta gcctccaggc cattgttttg tcgttcctga tccggccaac atgccggatc
    33241 ctgaaaaaaa ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga
    33301 aaagttttta cgcgttttta cctgcgccat gctttccaga atacggtgat agttttcgaa
    33361 acgggtttcc gcgattttcc cttcctcaac cgcttcccgg atagcgcagc ccggatcggt
    33421 atcgtgtttg caatcgcgat atttacacag acctaaatag tcatggaatt cgacaaagcc
    33481 ctgagtgatt tgttccggct ccaggtgcca gaggccgaac tcacgcacac caggggaatc
    33541 aatcacatca ccgccgtgcg ggaagtgata cagccgagcg gcggtcgtgg tgtgctgacc
    33601 gagccccgag ttgtcagaga catcgttggt caggatctct ttttgcagcc ccagcaacgc
    33661 attcagcagg ctggatttgc cgacgccaga ctgcccggca aaaatgctga tgcgcccggt
    33721 caacgcctct tccagcggtt ttagcccatc ctgagtatgg ctggaaacca tcaatacgcg
    33781 ataaccaatg ttgcggtaga tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc
    33841 cagcaggtct atcttattga gcacaataat cggctcaatc tgcaaggttt cgcaggccac
    33901 caggtaacgg tcgataatat tgagcgacag ctccggcaaa atggcggaga caatgacaat
    33961 ctggtcgatg ttggcggcaa taggtttcac gccgtcgtag aaatccggac gcgtcaacac
    34021 tgaggtgcgc tcatgcaccg cttccacgat ccctttgaca tttacgcctt ccgccgccgg
    34081 tttacccgga cgccagacta cgcggtcgcc ggttaccagc gaacggatgg tacggcgaat
    34141 attgcagcgg tgaacgtcgc catcggcgga ttccacatca gcgtgcatac caaagcggct
    34201 gatgacgata ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt
    34261 agacgtttta agacgacgct ggtgattggc gttcacgcgg cgctgctggc ctttggagag
    34321 tttattttta ctcaatcgta cagactcctg gtcgcccctg atgggcaaaa catctatgat
    34381 acacgcaatt gtggatcaat atagtcactg tgaatgggtg gaaaatagca tgagtgccaa
    34441 tgaaaacaac ctgatttgga tcgatcttga gatgaccggt ctggatcccg agcgcgatcg
    34501 cattattgag attgccacgc tggtgaccga tgccaacctg aatattctgg cagaagggcc
    34561 gaccattgca gtacaccagt ctgatgaaca gctggcgctg atggatgact ggaacgtgcg
    34621 cacccatacc gccagcgggc tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga
    34681 agctgaactg gcaacgctcg aatttttaaa acagtgggtg cctgcgggaa aatcgccgat
    34741 ttgcggtaac agcatcggtc aggaccgtcg tttcctgttt aaatacatgc cggagctgga
    34801 agcctacttc cactaccgtt atctcgatgt cagcaccctg aaagagctgg cgcgccgctg
    34861 gaagccggaa attctggatg gttttaccaa gcaggggacg catcaggcga tggatgatat
    34921 ccgtgaatcg gtggcggagc tggcttacta ccgcgagcat tttatcaagc tgtaaaattt
    34981 ttcctggtca cgtaagcgcc ggaatccact tgcggcggcg tgattttgac gctaaattga
    35041 acgtcttgct gataatttca gcacttgaga taaaaacgca aaaaaaactt ttttgggggg
    35101 ttgcagaggg aaagatttct cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa
    35161 ttacgaaagc aaaattaagt agtacgcggg aatagctcag ttggtagagc acgaccttgc
    35221 caaggtcggg gtcgcgagtt c
//
LOCUS       DAZFVO020000030        35177 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFVO020000030 DAZFVO020000000
VERSION     DAZFVO020000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.030"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..144
                     /locus_tag="ACHKNB_RS07075"
                     /old_locus_tag="pgaptmp_001415"
     CDS             <1..144
                     /locus_tag="ACHKNB_RS07075"
                     /old_locus_tag="pgaptmp_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4194965.1"
                     /translation="ASVHKFRQFRYIRFLNKRARKRLNTKLFKVQPYPKPQTVKTGLK
                     EIG"
     gene            complement(274..1473)
                     /gene="pbp4b"
                     /locus_tag="ACHKNB_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /pseudo
     CDS             complement(274..1473)
                     /gene="pbp4b"
                     /locus_tag="ACHKNB_RS07080"
                     /old_locus_tag="pgaptmp_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(1478..2902)
                     /gene="murP"
                     /locus_tag="ACHKNB_RS07085"
                     /old_locus_tag="pgaptmp_001417"
     CDS             complement(1478..2902)
                     /gene="murP"
                     /locus_tag="ACHKNB_RS07085"
                     /old_locus_tag="pgaptmp_001417"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HGU4194966.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(2906..3802)
                     /gene="murQ"
                     /locus_tag="ACHKNB_RS07090"
                     /old_locus_tag="pgaptmp_001418"
     CDS             complement(2906..3802)
                     /gene="murQ"
                     /locus_tag="ACHKNB_RS07090"
                     /old_locus_tag="pgaptmp_001418"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HGU4194967.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            3966..4823
                     /gene="murR"
                     /locus_tag="ACHKNB_RS07095"
                     /old_locus_tag="pgaptmp_001419"
     CDS             3966..4823
                     /gene="murR"
                     /locus_tag="ACHKNB_RS07095"
                     /old_locus_tag="pgaptmp_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HGU4194968.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            4952..5743
                     /gene="ucpA"
                     /locus_tag="ACHKNB_RS07100"
                     /old_locus_tag="pgaptmp_001420"
     CDS             4952..5743
                     /gene="ucpA"
                     /locus_tag="ACHKNB_RS07100"
                     /old_locus_tag="pgaptmp_001420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HGU4194969.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            5914..6930
                     /gene="cysP"
                     /locus_tag="ACHKNB_RS07105"
                     /old_locus_tag="pgaptmp_001421"
     CDS             5914..6930
                     /gene="cysP"
                     /locus_tag="ACHKNB_RS07105"
                     /old_locus_tag="pgaptmp_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HGU4194970.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            6930..7763
                     /gene="cysT"
                     /locus_tag="ACHKNB_RS07110"
                     /old_locus_tag="pgaptmp_001422"
     CDS             6930..7763
                     /gene="cysT"
                     /locus_tag="ACHKNB_RS07110"
                     /old_locus_tag="pgaptmp_001422"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HGU4194971.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            7763..8638
                     /gene="cysW"
                     /locus_tag="ACHKNB_RS07115"
                     /old_locus_tag="pgaptmp_001423"
     CDS             7763..8638
                     /gene="cysW"
                     /locus_tag="ACHKNB_RS07115"
                     /old_locus_tag="pgaptmp_001423"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HGU4194972.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            8628..9725
                     /gene="cysA"
                     /locus_tag="ACHKNB_RS07120"
                     /old_locus_tag="pgaptmp_001424"
     CDS             8628..9725
                     /gene="cysA"
                     /locus_tag="ACHKNB_RS07120"
                     /old_locus_tag="pgaptmp_001424"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HGU4194973.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            9859..10770
                     /gene="cysM"
                     /locus_tag="ACHKNB_RS07125"
                     /old_locus_tag="pgaptmp_001425"
     CDS             9859..10770
                     /gene="cysM"
                     /locus_tag="ACHKNB_RS07125"
                     /old_locus_tag="pgaptmp_001425"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HGU4194974.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(10773..11141)
                     /gene="yfeK"
                     /locus_tag="ACHKNB_RS07130"
                     /old_locus_tag="pgaptmp_001426"
     CDS             complement(10773..11141)
                     /gene="yfeK"
                     /locus_tag="ACHKNB_RS07130"
                     /old_locus_tag="pgaptmp_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HGU4194975.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            11246..12097
                     /gene="pdxK"
                     /locus_tag="ACHKNB_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /gene_synonym="thiJ"
     CDS             11246..12097
                     /gene="pdxK"
                     /locus_tag="ACHKNB_RS07135"
                     /old_locus_tag="pgaptmp_001427"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HGU4194976.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(12139..12648)
                     /gene="crr"
                     /locus_tag="ACHKNB_RS07140"
                     /old_locus_tag="pgaptmp_001428"
     CDS             complement(12139..12648)
                     /gene="crr"
                     /locus_tag="ACHKNB_RS07140"
                     /old_locus_tag="pgaptmp_001428"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HGU4194977.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(12689..14416)
                     /gene="ptsI"
                     /locus_tag="ACHKNB_RS07145"
                     /old_locus_tag="pgaptmp_001429"
     CDS             complement(12689..14416)
                     /gene="ptsI"
                     /locus_tag="ACHKNB_RS07145"
                     /old_locus_tag="pgaptmp_001429"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HGU4194978.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(14461..14718)
                     /gene="ptsH"
                     /locus_tag="ACHKNB_RS07150"
                     /old_locus_tag="pgaptmp_001430"
     CDS             complement(14461..14718)
                     /gene="ptsH"
                     /locus_tag="ACHKNB_RS07150"
                     /old_locus_tag="pgaptmp_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HGU4194979.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(15102..16073)
                     /gene="cysK"
                     /locus_tag="ACHKNB_RS07155"
                     /old_locus_tag="pgaptmp_001431"
     CDS             complement(15102..16073)
                     /gene="cysK"
                     /locus_tag="ACHKNB_RS07155"
                     /old_locus_tag="pgaptmp_001431"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGU4194980.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(16258..17019)
                     /gene="cysZ"
                     /locus_tag="ACHKNB_RS07160"
                     /old_locus_tag="pgaptmp_001432"
     CDS             complement(16258..17019)
                     /gene="cysZ"
                     /locus_tag="ACHKNB_RS07160"
                     /old_locus_tag="pgaptmp_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HGU4194981.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            17249..18235
                     /gene="zipA"
                     /locus_tag="ACHKNB_RS07165"
                     /old_locus_tag="pgaptmp_001433"
     CDS             17249..18235
                     /gene="zipA"
                     /locus_tag="ACHKNB_RS07165"
                     /old_locus_tag="pgaptmp_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HGU4194982.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            18306..20321
                     /gene="ligA"
                     /locus_tag="ACHKNB_RS07170"
                     /old_locus_tag="pgaptmp_001434"
     CDS             18306..20321
                     /gene="ligA"
                     /locus_tag="ACHKNB_RS07170"
                     /old_locus_tag="pgaptmp_001434"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGU4194983.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            20323..20541
                     /gene="ypeB"
                     /locus_tag="ACHKNB_RS07175"
                     /old_locus_tag="pgaptmp_001435"
     CDS             20323..20541
                     /gene="ypeB"
                     /locus_tag="ACHKNB_RS07175"
                     /old_locus_tag="pgaptmp_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HGU4194984.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(20538..21536)
                     /gene="yfeH"
                     /locus_tag="ACHKNB_RS07180"
                     /old_locus_tag="pgaptmp_001436"
     CDS             complement(20538..21536)
                     /gene="yfeH"
                     /locus_tag="ACHKNB_RS07180"
                     /old_locus_tag="pgaptmp_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HGU4194985.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            21626..22552
                     /gene="yfeR"
                     /locus_tag="ACHKNB_RS07185"
                     /old_locus_tag="pgaptmp_001437"
     CDS             21626..22552
                     /gene="yfeR"
                     /locus_tag="ACHKNB_RS07185"
                     /old_locus_tag="pgaptmp_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4194986.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(22591..23355)
                     /gene="yfeN"
                     /locus_tag="ACHKNB_RS07190"
                     /old_locus_tag="pgaptmp_001438"
     CDS             complement(22591..23355)
                     /gene="yfeN"
                     /locus_tag="ACHKNB_RS07190"
                     /old_locus_tag="pgaptmp_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HGU4194987.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            23604..24437
                     /gene="xapA"
                     /locus_tag="ACHKNB_RS07195"
                     /old_locus_tag="pgaptmp_001439"
     CDS             23604..24437
                     /gene="xapA"
                     /locus_tag="ACHKNB_RS07195"
                     /old_locus_tag="pgaptmp_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HGU4194988.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            24497..25753
                     /gene="xapB"
                     /locus_tag="ACHKNB_RS07200"
                     /old_locus_tag="pgaptmp_001440"
     CDS             24497..25753
                     /gene="xapB"
                     /locus_tag="ACHKNB_RS07200"
                     /old_locus_tag="pgaptmp_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HGU4194989.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVILMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            25806..25964
                     /locus_tag="ACHKNB_RS07205"
                     /old_locus_tag="pgaptmp_001441"
     CDS             25806..25964
                     /locus_tag="ACHKNB_RS07205"
                     /old_locus_tag="pgaptmp_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HGU4194990.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            26005..26889
                     /gene="xapR"
                     /locus_tag="ACHKNB_RS07210"
                     /old_locus_tag="pgaptmp_001442"
     CDS             26005..26889
                     /gene="xapR"
                     /locus_tag="ACHKNB_RS07210"
                     /old_locus_tag="pgaptmp_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HGU4194991.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(26998..27073)
                     /locus_tag="ACHKNB_RS07215"
                     /old_locus_tag="pgaptmp_001443"
     tRNA            complement(26998..27073)
                     /locus_tag="ACHKNB_RS07215"
                     /old_locus_tag="pgaptmp_001443"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27038..27040),aa:Lys,seq:ttt)
     gene            complement(27078..27153)
                     /locus_tag="ACHKNB_RS07220"
                     /old_locus_tag="pgaptmp_001444"
     tRNA            complement(27078..27153)
                     /locus_tag="ACHKNB_RS07220"
                     /old_locus_tag="pgaptmp_001444"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27118..27120),aa:Val,seq:tac)
     gene            complement(27200..27275)
                     /locus_tag="ACHKNB_RS07225"
                     /old_locus_tag="pgaptmp_001445"
     tRNA            complement(27200..27275)
                     /locus_tag="ACHKNB_RS07225"
                     /old_locus_tag="pgaptmp_001445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27240..27242),aa:Val,seq:tac)
     gene            complement(27320..27395)
                     /locus_tag="ACHKNB_RS07230"
                     /old_locus_tag="pgaptmp_001446"
     tRNA            complement(27320..27395)
                     /locus_tag="ACHKNB_RS07230"
                     /old_locus_tag="pgaptmp_001446"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27360..27362),aa:Val,seq:tac)
     gene            27653..29068
                     /gene="gltX"
                     /locus_tag="ACHKNB_RS07235"
                     /old_locus_tag="pgaptmp_001447"
     CDS             27653..29068
                     /gene="gltX"
                     /locus_tag="ACHKNB_RS07235"
                     /old_locus_tag="pgaptmp_001447"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGU4194992.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(29120..29512)
                     /gene="yfeD"
                     /locus_tag="ACHKNB_RS07240"
                     /old_locus_tag="pgaptmp_001448"
     CDS             complement(29120..29512)
                     /gene="yfeD"
                     /locus_tag="ACHKNB_RS07240"
                     /old_locus_tag="pgaptmp_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4194993.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(29514..29873)
                     /gene="yfeC"
                     /locus_tag="ACHKNB_RS07245"
                     /old_locus_tag="pgaptmp_001449"
     CDS             complement(29514..29873)
                     /gene="yfeC"
                     /locus_tag="ACHKNB_RS07245"
                     /old_locus_tag="pgaptmp_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HGU4194994.1"
                     /translation="MFKERMIPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            30094..30169
                     /locus_tag="ACHKNB_RS07250"
                     /old_locus_tag="pgaptmp_001450"
     tRNA            30094..30169
                     /locus_tag="ACHKNB_RS07250"
                     /old_locus_tag="pgaptmp_001450"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30127..30129,aa:Ala,seq:ggc)
     gene            30209..30284
                     /locus_tag="ACHKNB_RS07255"
                     /old_locus_tag="pgaptmp_001451"
     tRNA            30209..30284
                     /locus_tag="ACHKNB_RS07255"
                     /old_locus_tag="pgaptmp_001451"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30242..30244,aa:Ala,seq:ggc)
     gene            30492..32681
                     /gene="pdeA"
                     /locus_tag="ACHKNB_RS07260"
                     /old_locus_tag="pgaptmp_001452"
     CDS             30492..32681
                     /gene="pdeA"
                     /locus_tag="ACHKNB_RS07260"
                     /old_locus_tag="pgaptmp_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HGU4194995.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(32731..33933)
                     /gene="nupC"
                     /locus_tag="ACHKNB_RS07265"
                     /old_locus_tag="pgaptmp_001453"
     CDS             complement(32731..33933)
                     /gene="nupC"
                     /locus_tag="ACHKNB_RS07265"
                     /old_locus_tag="pgaptmp_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HGU4194996.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            34269..>35177
                     /locus_tag="ACHKNB_RS07270"
                     /old_locus_tag="pgaptmp_001454"
     CDS             34269..>35177
                     /locus_tag="ACHKNB_RS07270"
                     /old_locus_tag="pgaptmp_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HGU4194997.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSST"
BASE COUNT         8513 a         8621 c         9329 g         8714 t
ORIGIN      
        1 gccagtgttc ataagttccg acagtttcgt tacatccggt ttctgaacaa gagggccaga
       61 aaacgcctca acacaaagct attcaaagtc cagccttacc caaaaccaca aactgttaaa
      121 accggtttga aagagattgg gtgagtgctt aaaaactcat tctaaaacgc aaaggaaaag
      181 cgccgattta gtatcaaata agttgcgtat cggcgttttt cagcggtatc aaatgaaatg
      241 caaatcggta tcaaaaaaat cgccgcgcta tacaacaggc gaatgtggct tgttgcttaa
      301 catcacaatc gccatatgat tcaccggatc gataacggtc accgttccgg tccagccagt
      361 gtgaccgtag gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc
      421 attcacgcgc cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt
      481 caccgtttcc gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg
      541 cattaacacc gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc
      601 catcgaataa aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt
      661 cgggaaatgg atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg
      721 cggtttaaag cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata
      781 aatcgactct tcaacatagc ggtcaagcgg ctgaccggtg accgactcga cgataaatcc
      841 aagcagcata taatcgacat cactgtaaat atgcttgctg ccgggctgat actccagcgg
      901 cgtgcgcttg atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac
      961 cgctttattc ggatattgtg ggtccgccgg gaaaccgccg ctgtgatgca gcaggtcaga
     1021 aatccgcagc gtgtttttgc ctttgatggt gtcatttgga ctatcggcaa accccggaat
     1081 atatttcgca atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc
     1141 gaagttcgtg gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc
     1201 tttgacaggc tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt
     1261 gcgatacaca atctgattat ctttaatgat cagcaggttt acgccgggat aaccggcgtc
     1321 cacttgctgg ctaatccagc gatccatctg gttaagccgt tcgacgttga accctgcttt
     1381 ctctggcgaa ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc
     1441 cagcagagaa agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc
     1501 agccaaagag cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat
     1561 aaaccgccat tgccggaagg atgccttgtg cggaagtcat cagcggcagc gccaccagac
     1621 cagacggccc aaaggcgctg tttaagccca tcggcagacc ccaccaggct atcaggccga
     1681 taaacaaacc gcccgctgcg ccgcctaaac aggcggtaat aaacggtttc atgcggggca
     1741 gggtgacgcc gtaaatcaac ggttcaccaa cgcccagcag accgggaata atcgccccgc
     1801 gtacctgact gcgtaatgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc
     1861 ccacctggcc cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctggctgt
     1921 ccattaacgc gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca
     1981 ggaacagccc ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc
     2041 atgacatacc ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag
     2101 cggtgatcag cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc ggcataaagc
     2161 ggcgcaccat gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat
     2221 tgccgcgcgg atcgatgggc agaccaaaga aatcgtgaaa accggcgtag taaccggtgg
     2281 ttgcagcggg gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg
     2341 tgccgccaaa tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac
     2401 ctttgctgaa caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg
     2461 gaacgtgcat caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa
     2521 aaccggggat cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg
     2581 aggtttgttt ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta
     2641 ccggtgcctc gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc
     2701 caaaaacgac ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt
     2761 tgatattggg atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt
     2821 taccacaact ggcgatattt cccggtccgc cgacacgggt aagaatggtg ttcagaagtt
     2881 cactgctgat ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc
     2941 cgtgttgatc caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa
     3001 tggccgtttt acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac
     3061 atccggtggc gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga
     3121 ccacatcgac catcaggttg ccgaacactt tgccagattt aatcatcagc ccggtggaaa
     3181 gcatattgag caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca
     3241 cttcggcacc tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg
     3301 gattacagga aattcccact gtgcggcagc cgagctggcg tgcgtattcc agtccggcaa
     3361 tcacatacgg cgtgcgaccg ctggcagcaa tgccaaccac cacatcctgt gccgttaaat
     3421 taatattttt cagatcgtta acaccacctt cccggctatc ttcagcgcct tccaccgcgt
     3481 gctgaatggc atattcgcct ccagcaatca aaccaaccac cagacccggt ttcacgccgt
     3541 aggtgggcgg acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga
     3601 gataaatcag acgaccgccg ccgctgacct gggcgtggat aacatcgatc gccgcggcga
     3661 tatccggcag gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc
     3721 ggcacatttc cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag
     3781 taatcatctt ttctagttgc atcggtggtg accttactat ttacgatcaa ataattgcag
     3841 atgtatggaa tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacactcg
     3901 cagagtgtga agagcgtcat aagaacttaa gcggaattta tgctgccagc ccaggagagt
     3961 ggaagatgtt gtacctgacg aaaataagca atgcggggag tgaattcacc gaaaacgagc
     4021 aaaaaatcgc tgactttctg caggccaacg tgagtgagct gcaatccgtc tcttcgcgca
     4081 agatggcgaa acagctcggc atcagccagt cgagtattgt gaagtttgcc cagaagctcg
     4141 gcgcacaagg atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg
     4201 agaaaacgaa tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg
     4261 aagtgattgc ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgcgcgt
     4321 tgttcgacta cgcgcggctg caaaaaatca ttgatgtcat cagtaaagcg caatttattc
     4381 agataacggg cctgggcgga tcggcgctgg tggggcgcga tctgtcgttc aaactgatga
     4441 aaattggata tcgcgtggcc tgcgaagccg acacgcacgt acaagccacc gtttcccagg
     4501 cgttgaaaaa aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg
     4561 tcttatgcgc agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg
     4621 ctgattctcc gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg
     4681 aatggcgtag ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt
     4741 ttgttggtct tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg
     4801 agctaacaca acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat
     4861 tagtgagctg atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt
     4921 aacgctctgt attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact
     4981 gattacgggc gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg
     5041 cgcaaaccta atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg
     5101 tggtcgtggt catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc
     5161 cgcagctatc aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc
     5221 aggcgtttgt cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat
     5281 tgacatcaat attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc
     5341 ccgcaaagat ggtcgcattg tgatgatgtc ttcggtcact ggcgatatgg tggccgatcc
     5401 tggcgaaacg gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc
     5461 ggtggagtac gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac
     5521 accaatggcg gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac
     5581 tgaaatggcg aaagcaatcc cgatgcgtcg cctcgccgat ccgctggaag tcggcgaact
     5641 ggcggccttc ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga
     5701 tggcggcagc acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc
     5761 cctgcatgtg tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca
     5821 aatcaccaaa cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct
     5881 attagaaagt cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac
     5941 tcactcgcgc tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg
     6001 aacagttctt atgacgtctc ccgcgagctg tttgccgccc tgaatccgcc gtttgagcaa
     6061 caatgggcaa aagataacgg cggcgacaaa ctgacgataa aacaatctca tgccgggtca
     6121 tcaaaacagg cgctggcgat tttacagggc ttaaaagccg acgttgtcac ttataaccag
     6181 gtgaccgacg tacaaatcct gcacgataaa ggcaagctga tcccggccga ctggcagtcg
     6241 cgcctgccga ataatagctc gccgttctac tccaccatgg gcttcctggt gcgtaagggt
     6301 aacccgaaga atatccacga ttggaacgac ctggtgcgct ccgacgtgaa gctgattttc
     6361 ccgaacccga aaacgtcggg taacgcgcgt tatacctatc tggcggcatg gggcgcagcg
     6421 gataaagctg acggtggtga caaagccaaa accgaacagt ttatgaccca gttcctgaaa
     6481 aacgttgaag tgttcgatac cggcggtcgt ggcgcaacca ccacttttgc cgagcgtggc
     6541 ctgggcgatg tgctgattag ctttgaatcg gaagtgaaca acatccgtaa acagtatgaa
     6601 gcacagggct ttgaagtggt gatcccgaaa accaatattc tggcggaatt cccggtggcg
     6661 tgggtcgata aaaatgtgca ggccaacggt acggaaaaag ccgcaaaagc ctacctgaac
     6721 tggctctaca gcccacaggc gcagaccatc atcaccgact attactaccg tgtgaataac
     6781 ccggaagtca tggacaaact gaaaaacaaa ttcccgcaga ccgagctgtt ccgcgtggaa
     6841 gacaaatttg gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta
     6901 gacaagctgt tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc
     6961 cgggctttac cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc
     7021 cgctctccgc gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga
     7081 tcaccaaccc gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat
     7141 cgatttttaa cggcgttttc ggtctgctga tggcgtggat cctaacccgc tatcgcttcc
     7201 caggccgcac gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg
     7261 ccggtttaac gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga
     7321 agtttgatat caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca
     7381 gcattccgtt tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg
     7441 aagaagcggc ggaaacgctt ggtgcaacgc gctggcagag tttctgcaaa gtggtgctgc
     7501 cggagctttc tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg
     7561 aatttggcgc ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc
     7621 tgatgatttt tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg
     7681 tgatcctcgc ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg
     7741 gtcggcgtgt ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg
     7801 cccgattaac tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat
     7861 cctgctggtg ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt
     7921 tttacagaat ctggccgatc cggacatgct acacgccatc tggctgacgg tgatgatcgc
     7981 gctgattgcc gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg
     8041 ctttaacttc cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc
     8101 gccggtggtt gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg
     8161 ttggctggat gaacataatc tgcaaattat gttctcctgg ccgggaatgg tgctggtcac
     8221 catcttcgtg acgtgtccgt ttgtggtgcg cgaactggtg ccggtgatgt taagccaggg
     8281 cagtcaggaa gacgaggcgg cgattttgct tggcgcgtct ggctggcaga tgttccgtcg
     8341 tgtcacattg ccgaacatcc gctgggcgct gctttatggc gtagtgctga ccaacgcccg
     8401 cgcgattggc gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaacact
     8461 ttcgctgccg ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac
     8521 cgctgcggca ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca
     8581 gtggcgcctg gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga
     8641 ttgccaatat taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata
     8701 ttccttcagg tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc
     8761 tgcgcattat cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg
     8821 acgtcagccg cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc
     8881 tgttccgcca tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc
     8941 gcgagcgccc gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gaaatggtcc
     9001 agcttgcgca tctggcggat cgttatccgg cgcagctttc cggcggccag aaacagcgtg
     9061 tggcgctggc gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg
     9121 gcgcgctgga tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag
     9181 agctgaaatt caccagcgtg tttgtgaccc acgaccagga agaggcgacc gaagtagccg
     9241 atcgtgtagt ggtgatgagt caggggaata ttgaacaggc tgatgcgccg aatcaggtat
     9301 ggcgcgaacc agcgacccgt tttgtactcg aatttatggg cgaagtgaac cgcctgcagg
     9361 gaaccattcg cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac
     9421 ctgcttatca ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc
     9481 gtaccagtct cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact
     9541 acacccaatt agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc
     9601 atggcgacga tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc
     9661 tgtataacgg cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg
     9721 cctgataggt tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta
     9781 atggcaaggt ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac
     9841 cacgtgtgga tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg
     9901 aagttgcagc gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat
     9961 aacccggcag gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag
    10021 cgcggggaaa ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt
    10081 gcgctggcaa tgattgccgc gcttaaaggc tatcgcatga aattgctgat gcccgacaac
    10141 atgagccagg aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc
    10201 aaagagcagg gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa
    10261 ggaaagctgc tcgatcagtt caataatccc gataaccctt atgcgcatta caccaccacc
    10321 gggccggaaa tctggcagca aaccggcggg cgcatcactc attttgtctc cagtatgggg
    10381 acgaccggca ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accggtgacc
    10441 attgttggcc tgcaaccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg
    10501 gaatatctgc cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag
    10561 cgcgatgcag aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc
    10621 agctccggcg gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg
    10681 gtggtagcga tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa
    10741 gagcatttta gccagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc
    10801 tgcgcaatta gcgcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc
    10861 acaatatacg gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg
    10921 gcagtgtcaa tgcggttacg ggtattgccc agcttcagac gcagatgaga aaccgcttca
    10981 tcgcaggtat gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc
    11041 tccagcatgg cgttgatgcg ggcttcttca tgggcagtta acttcgcgta agcggggagt
    11101 gtcattagta gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt
    11161 tttgtgatag ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg
    11221 atcatcggca aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagcag
    11281 ggcgctgcag gcggatatcg tcgccgtgca gtcgcaggtg gtttacggca gcgtgggcaa
    11341 cagcattgcc gtgcctgcta tcaaacagaa cggtctgaat gtttttgccg tgccgacggt
    11401 attgctgagc aatacgccgc attatgacac tttctacggt ggtgcgattc cggatgaatg
    11461 gtttagcggt tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccagc tgcgcgctgt
    11521 aaccacgggc tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc
    11581 gctacgcaaa gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga
    11641 tagcggaatt tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct
    11701 ggcgcaggga attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg
    11761 cgatctcgac agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt
    11821 ggtgattacc agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag
    11881 tgccgacagc gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg
    11941 cgacctgttt tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc
    12001 ggtgcaccgt gcggggttgc gcgtactgga agtgatgcgc tacacccagc agcatgagag
    12061 cgatgaattg attttgccgc cgctggcgga agcataaaaa atggcgccga tgggcgccat
    12121 ttttcactgc ggcaagaatt acttcttgat gcggataacc ggggtttcac ccacggttac
    12181 gctaccggac agtttgatca gttctttgat ttcgtccatg ttggagataa caaccggagt
    12241 cagggtagac ttggctttct cttccagtag cggcagatca aattcaatga cagtatcgcc
    12301 aactttcacg cgctgacctt cttcagcaat acgcttgaag ccttcgcctt tcagttcaac
    12361 ggtgtcgata ccgaagtgga cgaacagttc aacgccgcta tcagattcga tagagaacgc
    12421 gtgattggtt tcaaagattt taccaatggt accgtctaca ggcgcgacca ttttgttacc
    12481 cgttggtttg atagcaatac catcaccaac gattttttcc gcaaaaacga catccggcac
    12541 gtcttcgata ttgacgatct cgccagagag cggagcaatg atctcaatag ttccggtatc
    12601 cttcttgtcg tcggaaacca gagatttcag tttatcgaac aaacccatga tcttctccta
    12661 agcagtaaat tgggccgcat ctcgtggatt agcagattgt tttttcttca atgaacttgt
    12721 taaccagcgt cattaactcg tccgttgtcg gttgagcaag agcctgctct gctaacacct
    12781 tcgcatcttc gaagttcgtg ttacggataa tcttcttaat gcgcgggata gaaatggcgc
    12841 tcatagagaa ttcgtccaga cccatcccca gcaacagaag tgtagcacgt tcatcgccag
    12901 caagctcacc acacatgcca gtccatttgc cttcagcatg agaagcatca ataacttgct
    12961 tgatcaagtt cagcacggac ggtgacattg gttggtaaag gtgtgaaatc atatcattac
    13021 cacggtcaac tgccagagtg tactgcgtta aatcattggt gccgatacta aagaaatcaa
    13081 cttctttggc taaatgacgt gcaattgttg cggcagccgg tgtttccacc attacgccga
    13141 tttcaattga ctcgtcaaac gctttacctt cgtcgcgcag ttcctgtttg tagatttcga
    13201 tctctttgcg cagtgcacgc acttcttcaa cagagatgat catcgggaac ataatgcgca
    13261 atttaccgaa agccgaggca cgcaggatag cgcggagctg atcgcgcagg atctctttac
    13321 gatccatcgc gatacggata gcgcgccagc cgaggaacgg gttctcttct ttcgggaagt
    13381 tcatgtatgg cagctctttg tcgccgccga tgtccatggt acgaacgata accgcctgcg
    13441 agccacacgc ttcagccact gctttgtaag cagcaaactg ttcttcttca gtgggcagcg
    13501 cgtcgcggtc catgaacagg aactcagtac gatacagacc aacgccttca gcgccgttac
    13561 gctctgcacc ttcaacgtca cgaaccgtac caatgttagc gcacacttct acctggtgac
    13621 cgtccagcgt aatagctggc agatctttca gtttagcaag ctctgctttt tcagaagcca
    13681 cttgctcctg aacagcgcgc attttatcaa taacttcgtt ggttggattg acgtaaacct
    13741 gattatttac ggcatccaga atcagatagt cgtcattttt cacctgagag gtgacgctac
    13801 cggtacccac gatagcaggc agttccagag aacgcgccat gatagaggtg tgggaagtac
    13861 ggccacccgc gtcggtgatg aaacccagca ccttcttcag gttcagctgt gcggtttcgg
    13921 acggcgtcag gtcagcggca accagaatga cttcatcctg aatggcgctc aggtcgataa
    13981 tcttcaggcc caggatgttg cgcagcaggc gcttaccgat atcacgtacg tcagccgcac
    14041 gttctttcag gtattcatca tccagctctt ccagggcaga agcctgacct tcgataactt
    14101 catgagcagc tgcgtcagct gtcatgtgct tatctttaat cagggctatg atttcctgct
    14161 ccagctcctc atcttcgagc agcataatat gcccttcaaa gatggcttct ttttcttcac
    14221 cgaacgtttc accagctttc gttttgatcg tttccagctg ggctgatgcc ttggcacgac
    14281 cgctcagaaa acgttcaact tcctgatcaa cctggtcggc agaaattttt ttccggtcaa
    14341 tgacaatttc gtcttctttc agaagcagag ctttaccgaa agcgataccc ggggatgcta
    14401 aaatgcctga aatcataacc ctaccttact tgtgactgat ttttaaaaga acccgggaaa
    14461 ttactcgagt tccgccatca gtttaaccag atgttcaacc gctttctgct cgtcttcgcc
    14521 ttctgcggag atagtcacaa cggtaccttg agtcaggccc agagtctgca gtttaaacag
    14581 gcttttcgcg ctggcgcttt tgccgttgga agtcacagta atttcagaag tgaagccctt
    14641 agcttctttt acaaactggg cagcagggcg ggtgtgcaga ccgttcggag cggtaatggt
    14701 aacttcttgc tggaacattg tatttcccca acttataggt ttagtgttgt ggaactaaag
    14761 tctagcctgg cggttcgact ttagcctgta ttgttagcgc cagtttttaa cagacgcgac
    14821 gcacgaagag cgcaatacag tccattgctg gcgggtgttg accgctgacg tttcgtacgt
    14881 cattaaacat tatgccgcta aaggaagaat tgaaccaaat cataaaatcg attcagcttt
    14941 ggcttttcct gtaacgatta atttcgcgca tcaaaataat tagtctggtt aaataccagt
    15001 tccgacaggc tgaatcaatg ccaggagggg cgaaagtttg aagcaggcca caaaaaagca
    15061 cctaaaaagg tgctttttta cgcattttta acaagctggc attactgttg cagttctttc
    15121 tcagtgaaga gatcggcaaa caatgcggtg cttaaataac gctcacccga tgacggtaga
    15181 ataaccacaa tattcttgtt tgtaaagctt tcatcttctt gtagtttcaa cgccgcagca
    15241 acagctgctc cagaagagat acctgcaaga ataccttctt cttccatcag acgacgcgcg
    15301 gtagaaatcg cttcttcatt ggtgatgcca atgactttat cgaccagctt gagatcgagg
    15361 ttagctggga taaaaccagc gccaataccc tgaattttat gcgggccagg tttaatctct
    15421 tcaccagcca gtgcctgggc gataactgga gaatcggttg gctcaacggc gacagagata
    15481 agatcggtct tgcctttggt gcctttaatg tagcggctga cgccagtaag cgtaccgcca
    15541 gtcccaacgc cagcaataaa tacatcaacc tgaccgtcgg tatcttccca gatctccgga
    15601 ccggtggttt tttcgtgaat ttcagggttt gccggattgc tgaattgttg cagcagcagg
    15661 tatttctctg gattgctggc gacaatttct tctgcttttt ggattgcgcc tttcatgcct
    15721 ttagcacctt ccgtcagcac caggtttgca cctaacgctt tcagcagctt gcggcgttca
    15781 atactcatgg tttctggcat ggtcagggtg agtttgtaac cgcgagcggc agctacatag
    15841 gccagtgcaa tcccggtatt accgctggtt ggttcaacca gttcaacgcc tggtttcagc
    15901 acgccgcgct tttcggcatc ccaaatcatg ttggcaccga tacggcactt aacgctgaag
    15961 ctggggttac gagattccac cttcgccaga atgcgtccgt taccgatgcg attcaggcga
    16021 accagcggcg tgtgaccgat agtcagcgag ttatcttcaa aaatcttact catggcctgt
    16081 ccttaactgt atgaaattgg gatacaacag gtagcatacc cgctcagaga atatgcggaa
    16141 gtaaggattt agcatatcta tatacagaag ggaaataatg acatgcaaga tggaataagg
    16201 ggcggcataa gccacccctg tttcacacaa acggtttaca aaataaccgg tagattgtta
    16261 ccgccacatc gcgtgtttat cgcgatagca atcgacccac atcgccgtcg cgccacaaac
    16321 ggcaacgggc atgatgaaca gattaagcag cgggatcatc gtaaacaggc tggttaaagc
    16381 accaaactgc atattggtga ttttgcgtgt gcgcagggcg gtgcgcatct ctttaaacgg
    16441 cactttgtgg ttatcgaagg ggtaatcgca atactggatg gctaacatcc aggcgctaaa
    16501 caggaaccac agtaccggcg cgacggtttg cccaatacca gggattaagt aaagaattag
    16561 caatacaatt gcgcgcggca gataccaggc aaatttttgc cattcgcgtt tcatgattcg
    16621 cggcacatct ttcatgatac cgaaaatccc ggtatctggc ggtgtagcgc cagtcaatcg
    16681 tgcttccagt tgttcagcca ataaaccgtt aaacggagcg gcaatccagt tagcaatcgt
    16741 ggagaagaaa tagccaaaca ctaacagcac agagatgaca gccagaggcc acaacagata
    16801 actcagccat tgtagccagt ccggaacgta actcatgaga gtcgggatcc agacatcgag
    16861 ctgtgtaaag agccaccaga atgcgccccc catcaacaaa atattgacca gcagcggtaa
    16921 aataacgaaa cgccgaatcc caggttgcga gacgagcttc cagccttgcg caaaatagta
    16981 aaaaccgctg cgtggggcag atgtgaatga tgaaaccata atcaggatga gctccttttg
    17041 accaatccca ggaaaattct gcgtatttta ccgggtaatt gcgcaatgga cagttaggat
    17101 atgttcgaaa aaacagcaaa aagcacgatt tcatctatct ttgtgctgtg aaagttaata
    17161 gtgcacttgc acttgaggta atcggcaaat actcttagtg agtaaatgtt tgccgtggtg
    17221 gcaaggtgtt agaacaacag agaatataat gatgcaggat ttgcgtctga tattaatcat
    17281 tgttggcgcg atcgccataa tcgctttact ggtacatggt ttctggacca gccgtaaaga
    17341 acgatcttct atgttccgcg atcggccatt aaaacgaatg aagtcaaaac gtgacgacga
    17401 ttcttatgac gaggatgtcg aagatgatga gggcgttggt gaggttcgtg ttcaccgcgt
    17461 gaatcatgcc ccggctaacg ctcaggagca tgaggctgct cgtccgtcgc cgcaacacca
    17521 gtaccaaccg ccttatgcgt ctgcgcagcc gcgtcaaccg gtccagcagc cgcctgaagc
    17581 gcaggtaccg ccgcaacatg ctccgcgtcc agcgcagccg gtgcagcagc ctgcctatca
    17641 gccgcagcct gaacagccgt tgcagcagcc agtttcgcca caggtcgcgc cagcgccgca
    17701 gcctgtgcat tcagcaccgc aaccggcaca acaggctttc cagcctgcag aacccgtagc
    17761 ggcaccacag cctgagcctg tagcggaacc ggctccagtt acggataaac cgaagcgcaa
    17821 agaagcggtg attatcatga acgtcgcggc gcatcacggt agcgagctaa acggtgaact
    17881 gcttcttaac agcattcaac aagcgggctt catttttggc gatatgaata tttaccatcg
    17941 tcatcttagc ccggatggca gcggcccggc gttattcagc ctggcgaata tggtgaaacc
    18001 gggaaccttt gatcctgaaa tgaaggattt cactactccg ggtgtcacca tctttatgca
    18061 ggtaccgtct tacggtgacg agctgcagaa cttcaagctg atgctgcaat ctgcgcagca
    18121 tattgccgat gaagtgggcg gtgtcgtgct tgacgatcag cgccgtatga tgactccgca
    18181 gaaattgcgc gagtaccagg acatcatccg cgaagtcaaa gacgccaacg cctgatacac
    18241 ttaaggcaaa ttaactcctc ttcgaacccc cgcttgtcgg gggtttttag cattgatggt
    18301 gcgatatgga atcaatcgaa caacaactga cagaactgcg aacgacgctt cgccatcatg
    18361 aatatcttta tcatgtgatg gatgcgccgg aaattcccga cgctgaatac gacaggctga
    18421 tgcgcgaact gcgcgagctg gaaaccaaac atccagaact gattacgcct gattcgccta
    18481 cccaacgtgt aggcgctgcg ccgctggcgg ctttcagcca gatacgccat gaagtaccaa
    18541 tgctgtcact ggataacgtt tttgatgaag aaagctttct tgctttcaac aaacgtgtgc
    18601 aggaccgtct gaaaaacaac gagaaagtca cctggtgctg tgagctgaag ctggatggcc
    18661 ttgccgtcag tattctgtat gaaaatggcg ttttagtcag tgccgcgacc cgtggcgatg
    18721 gcaccaccgg ggaagatatc acgtctaatg tgcgtactat tcgcgctatt ccgctgaagc
    18781 tgcacggaga gaatatcccg gcgcgtctgg aagtgcgtgg tgaagtgttc ctgccgcagg
    18841 cggggttcga aaagattaac gaagatgcgc gacgcacggg cgggaaagtt tttgctaacc
    18901 cacgtaatgc ggcagctggt tcactgcgtc agcttgatcc gcgtattaca gcgaagcgac
    18961 cgctcacttt tttctgctat ggcgttggtg ttctggaagg tggcgagctg ccggatactc
    19021 atcttggccg tttactgcaa tttaaaaagt gggggttgcc ggtcagcgat cgggtaacgc
    19081 tttgtgaatc ggcggaagaa gtgctggcgt tctatcacaa agtggaagaa gaccgcccga
    19141 cgctgggctt tgatatcgac ggcgtggtga ttaaggtcaa ctcactggaa cagcaggagc
    19201 agcttggctt tgtcgcgcgt gccccgcgct gggcggtagc gtttaaattc ccggcgcagg
    19261 agcagatgac ctttgtgcgt gacgtcgagt ttcaggttgg gcgtactggc gcgattacgc
    19321 ctgttgcgcg tctggaacct gtccatgttg ctggcgtgct ggtgagtaac gcaaccttac
    19381 acaatgcgga tgaaatcgaa cgtcttggtt tacgcattgg cgataaagtg gtgattcgcc
    19441 gcgctggcga cgtgatcccg caggtggtta acgtcgtgct ttctgaacgc ccggaagata
    19501 cccgtgaggt tgtattccca acgcattgtc cggtatgtgg ttctgacgtt gagcgtgtgg
    19561 aaggtgaagc ggttgcccgc tgtaccggtg gcctgatttg cggtgcgcag cgtaaagagt
    19621 cgctgaaaca ctttgtttcc cgccgtgcga tggatgttga cggaatgggc gacaaaatca
    19681 tcgatcagct ggttgaaaaa gaatatgtcc atactccggc agatctgttc aaactcaccg
    19741 caggcaaact gaccggactg gagcgtatgg ggccaaaatc ggcacaaaac gtggttaacg
    19801 cgctagaaaa agcgaaagaa accacctttg ctcgcttcct ctatgcactt ggcatccgtg
    19861 aagtcggcga ggccaccgca gcaggtctgg cggcatattt cggcacgctg gaagcgctgg
    19921 aagccgcttc gattgaagag ctgcaaaagg tgcctgatgt tggcattgtc gttgcatccc
    19981 acgttcacaa cttctttgcc gaagaaagca accgcaatgt tatcagcgag ctgttggcgg
    20041 aaggtgtcca ctggcctgcg ccgatcgtta tcaacgcgga agagattgac agcccgtttg
    20101 ctggtaaaac cgtggtgctt acgggcagct taagtcagat gtcacgtgat gacgctaaag
    20161 ctcgactggt cgaactgggc gcgaaagtcg cgggcagcgt gtcgaagaaa accgatctgg
    20221 tgatagcggg tgaagctgca ggatctaaac tggcgaaggc gcaggaactg ggcattgaag
    20281 tcatcgacga agcggaaatg ctgcgtttgc tgggtagctg agatggaaaa agagcagctg
    20341 attgaaatag ccaatacgat aatgccgttt ggcaaataca aagggcgtcg cttaatcgac
    20401 ctgccggagg agtatctgct gtggtttgcc cgcaaagatg agttcccggc agggaagctc
    20461 ggtgagctaa tgcaaatcac gctgctgatt aaaaccgagg ggctgacgca actggtccag
    20521 cccctgaagc gtccgcttta agctttatcg gtgctgcttt cctgctgcgc ctgtaactgt
    20581 tcggtctggc gtttgtatcg acgcgccagc accgcacaga ccatcaattg gatctgatgg
    20641 aaaatcatca ggggcagcac catcataccg atcaccgatg tggggaacag aatgtttgcc
    20701 atcgggatgc catttgccag actctttttc gaaccacaaa agacgatagt aatttcatct
    20761 gccttattga agcccagtcg gcgtgccata aagacgttaa ctacaatcac gatagccaga
    20821 agaacgcagc tgaccaccac gataaacagc aatgatcccc agccaacttt atgccagata
    20881 ccattaacga cggcttcgct gaacgctgta taaaccacca acagaatgga cgtctggtca
    20941 gttttcgcaa tccatttttt attgcgcgac acccagtcac caatccacgg ccgggaaaga
    21001 tgccccaaca caaacggcag cagcagttgc agcataattt taccgacctg ctcaaggctg
    21061 ccccctgcac cgtgaacatt catcaccaga ccaaccagca atggtgaaag gaaaatcccc
    21121 agcaggctgg atgctgacgc agaacaaacc gccgccgcga cgttaccgcc cgccattgac
    21181 gtgaaggcga ttgcagactg cacggtagcc gggagaatgc acaagtagag aaaaccggag
    21241 tagagcatcg ggtcgacatt taccggtttc caccaggcaa acagtacacc cagaatcgga
    21301 aacagcacga aggtgctgca cattacccac aaatgcagtc gccagtgacc accgccagca
    21361 ataatcgcct cacgcgacaa cttcgcgccg tgcataaaga acagcagggc aatcgctgcg
    21421 gtggtcagat tttcaaagaa ggggacgaaa tcgcctctgg ccggaaagaa agaggccagc
    21481 aacaccaccg tgatcagggt taaggtgaaa ggatcgagga tacgaaaaag tttcataaag
    21541 actccagatg atcgatggtt ctattgtgct tcttccagtt tgagaaataa aattgattta
    21601 ttgcatccat tcatgaagaa aacagatgaa ttattcttta aaacaattaa aggttttcgt
    21661 cacagtagcg caggagaaaa gttttagtcg tgcaggagag cgtatcggcc tgagccagtc
    21721 ggcagtgagt cacagtgtga aggaactgga aaatcatact ggtgttcgcc tgctggacag
    21781 aaccacgcgt gaagtggtgc ttacagatgc agggcagcag ctggctttgc gtcttgagcg
    21841 actgctggat gaactgaaca gcacgttgcg cgataccggg cgtatggggc aacaactgag
    21901 cggaaaagtt cgggtcgctg ccagccagac catttccgcg catcttattc cgcaatgcat
    21961 tgctgaaagc catcgccgct atccagatat tcagtttgtc ctgcacgatc gtccgcagca
    22021 gtgggtgatg gaaagtattc gccagggaga tgtcgacttt ggcatcgtca tcgatccagg
    22081 cccagtgggc gatctgcaat gtgaagcgat tctttccgag cctttctttc tgctttgcca
    22141 tcgcgatagc gctttggccg tcgaagatta tgtaccctgg caggcattac agggagcgaa
    22201 attggtgttg caggattacg cgtcaggcag ccgaccgctg attgacgcag cgctggcgcg
    22261 caatggtatt caggcgaata ttgtgcagga gattggacat ccggcgacgc tgttcccgat
    22321 ggtagccgca ggcatcggca tcagtattct tcccgcactg gcgctaccat tgcctgaagg
    22381 tagcccactg gtggtaaagc gcattacgcc ggttgttgaa cgccagttga tgctggtgcg
    22441 caggaagaac cgttcacttt ccaccgccgc tgaagcgtta tgggacgtgg tacgagacca
    22501 gggcaatgcg ctaatggctg gtcgggaagg ggatccgttg tatcagatat agagtgaatt
    22561 catactgccg ggagccccgg cagtaatcaa tcagaagtta aatttgatgg agtagattat
    22621 cgcatcctgg taaaaatctt cgcccagttt gtcatccgca tatcgatact gaatccccgt
    22681 agtaatgtgt gatgttgcat tccaccacag tgcaactgcg ccattcagcc cttctttacc
    22741 gccattaccc gctgcatagg tagcgtcacg gtcaaactcg tactcgttcc agttggtaag
    22801 agtgaatttc tcgctgccca gcataaagtt gtaacctgca acccagccgg cgacatagcc
    22861 gttgtcgcca gtgtaatagg tttgatctgt ataacgctta gcaaagaacg gtttgaacca
    22921 ccaaccgctg ccggtgaaat tgtagccgat accgtacagg aacatatcgt cgtggaaatt
    22981 cacgcgattc gcagaaccgt aggtgccata cgcatgcaag tagagattaa atccggtgtc
    23041 acccaggtaa atacggttgg tatttttaaa ggtataacgc tgctcgctac ctggtttatt
    23101 atggcgaccg ttatagaagt tttcccagtc gaagaacccg tacatttctc cccaactaaa
    23161 gttagcgcca ccttcaaatt caagataacc aaaatcatct ttgtgtgatt tggtcgacga
    23221 tttttcggta gtccggctgg tccagtccag ataatgcacg ccgatatctg caaaaccgcc
    23281 tttaaattgt gcatgagata caacaggtat cgctaatata gaggaaagtg tcagagttaa
    23341 aagatgtttt ttcatagcat aattccctat gccgatcgtt attagtagat tgcttaataa
    23401 agtggtttgc taaaaacgac atattgttaa tgcaaaggaa ttaatatcgc caatacagtt
    23461 tttaccgtgg attaatagag aaaagatata aatccatcga tatcgaatcg ctattgaatg
    23521 cgtgagaaaa gtgtattggt aagagccggg acaacctcgc tataaaaagg cgttagattc
    23581 caccctacag aaaaaggata tgtatgtctc aggttcaatt ttctcataac ccgctgtttt
    23641 gcatagatat tatcaagact tataaacctg atttcacgcc acgagtggcc tttattttag
    23701 gttccgggct cggcgcgctg gccgatcaga ttgagaacgc tgtcgcaatt tcctacgaaa
    23761 agctgccggg cttcccggta agtacggtac atggtcatgc gggggagtta gtgctgggcc
    23821 atctccaggg cgtaccggtg gtatgtatga aaggtcgcgg acatttctac gaaggtcgtg
    23881 gaatgaccat tatgactgac gcaattcgta cctttaagct gctgggctgc gagctactgt
    23941 tctgcaccaa tgcggcaggc tcgctgcgtc cggaagtggg ggcaggcagc ctggtcgcat
    24001 tgaaagatca tatcaacacc atgcccggta cgccgatggt gggtcttaac gatgatcgtt
    24061 ttggagagcg cttcttctca ctggcaaatg cctacgatgc ggaataccgc gcactgttac
    24121 aaaaagtggc gaaagaagag gggttccctc tgacggaggg cgtgttcgtc tcgtatccgg
    24181 ggccgaattt cgagactgcg gcggaaattc gcatgatgca aattattggt ggggatgttg
    24241 ttggtatgtc tgtggtgcct gaggttattt cagctcgcca ttgcgacctt aaagtcgttg
    24301 cggtctctgc gattaccaat atggcggaag gtttaagcga tgtgaaattg tcacatgcgc
    24361 aaacgctggc ggctgcggag ctctctaagc agaacttcat caaccttatt tgtggctttc
    24421 tgcgcaaaat tgcctgagaa aaacaataag aaacggcctc gcgggtgagg ccaaatgatg
    24481 cgacaaggaa acgattatga gcatcgcgat gcgcttaaag gtaatgtcct ttttgcaata
    24541 ttttatctgg gggagctggc tggttaccct cggctcttac atgattaata cccttcattt
    24601 caccggcgct aatgttggca tggtttacag ttccaaaggg atcgccgcga ttattatgcc
    24661 tggtataatg gggatcatcg cagacaaatg gctgcgcgca gaacgtgcat acatgctgtg
    24721 tcacctggtg tgtgcgggcg tactttttta tgcggcatcc gtaactgatc cggatatgat
    24781 gttttgggtg atgttagtca atgcgatggc gtttatgccg actattgcgt tatcgaacag
    24841 cgtctcttat tcctgtcttg cccaggcagg gcttgacccg gtgaccgctt tcccgcccat
    24901 tcgcgttttt ggtacggtgg ggttcattgt cgcgatgtgg gcagtaagcc tgctgcatct
    24961 ggaattgagt agtctgcagc tgtatatcgc gtccggtgcg tcattgctgc tgtcggctta
    25021 tgcgctgact ttgccgaaga ttccggttgc ggagaaaaaa gcgaccacat cgcttgccag
    25081 caagctgggt ctggatgcct tcgtgctgtt taaaaatcca cgcatggcca tctttttcct
    25141 ctttgccatg atgctgggtg cggtactgca aattaccaac gtttttggta atccgttcct
    25201 acatgatttc gcccgtaacc cggagtttgc tgacagtttt gtggtgaaat atccctccat
    25261 tttactgtca gtttcacaga tggcagaagt gggctttata ctgactatcc cattcttttt
    25321 aaagcgattt ggcattaaag ccgtcatact gatgagtatg gtggcctgga cgctgcgctt
    25381 tggcttcttc gcctatggcg atccgtcaac aaccggattt attttgctgc tgctgtcgat
    25441 gattgtttat ggctgtgcat tcgatttctt caatatttct ggttcggtat ttgtcgaaca
    25501 ggaagttgat tccagcattc gtgccagcgc gcaggggctc tttatgacca tggtaaatgg
    25561 tgtcggcgca tgggttggct cgattctgag tggcatggca gtagattact tttcggtgga
    25621 tggcgtaaaa gactggcaaa ctatctggct ggtgtttgca ggatatgctc tttttctcgc
    25681 agtgatattt ttctttgggt ttaaatataa tcatgaccct gaaaagataa agcatcgagc
    25741 ggtggctcat taaaaggatt cgcagctctg ctcttcagag ctgcttttat gataaaggtt
    25801 aattagtgaa agatatttta ttttcttccg gcgttggttt tggcattggt gctttgttta
    25861 caatcgtgcg cttgcctatt cctgtaccaa atgtattacc aggtatatta tccattgtgt
    25921 ttatgtatgt cggatatctg gtggtgaaat actttatgcc atgataattt aatacgatgt
    25981 atttattata tggagcactt aattatggaa cgcgtataca gaacagatct taagttgctc
    26041 cgttattttc ttgccgtagc ggaagagttg cattttggcc gcgcagcagc gcgtttaaat
    26101 atgtctcagc ctccgctcag tattcatatt aaagagctgg aaaatcaact cggcacgcag
    26161 ctttttattc gccattcgcg cagcgtcgta ctgacacacg cgggcaaaat cttgatggaa
    26221 gaatcgcgtc gattgctggt gaatgctaat aatgtattgg ctcgggttga acaaataggt
    26281 cggggagaag cagggcggat tgaactcggc gtagtgggaa cggctatgtg gggacggatg
    26341 cgcccggtta tgcggcgatt cctcagggaa aatcctaacg ttgaagttct ttttcgcgaa
    26401 aagatgcccg cgatgcaaat ggccctgctg gaacgacgtg aactcgatgc cgggatctgg
    26461 cgaatggcga cagaaccacc gacaggtttt accagcttac ggttgcatga atcggcgttt
    26521 ctggtggcga tgcctgaaga gcatcatctc tcatcatttt ccaccgttcc gctggaagcg
    26581 ctacgtgacg agtattttgt tacaatgccg cccgtgtaca ctgactggga ttttttgcag
    26641 cgagtttgcc agcaggtggg attttcaccg gttgttattc gcgaagttaa tgaaccgcaa
    26701 acggtactcg ccatggtcag tatgggtatt ggtatcacat tgatagcgga cagctacgca
    26761 caaatgaact ggccaggtgt cattttccgt ccgctcaaac agcgcatccc tgcagattta
    26821 tatattgttt atgaaacaca gcaggtgacg cccgcgatgg ttaagctgtt ggctgcattg
    26881 acacagtagg tgaggagaca ggaattgccg gaaatagtcg tctgctaagt gaagcgcatc
    26941 aggcaatttc gcaaatcgca gatagcaaaa aagcgccttt agggcgcttt tttacattgg
    27001 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
    27061 gagctaacga cccgaagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
    27121 agggaggtgc tctcccagct gagctaatca cccacttagg tacttgacat ttttacaaga
    27181 aatttagctg gcgagaaagt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
    27241 taagggaggt gctctcccag ctgagctaat cacccacttc tcaatttctt gctacacggc
    27301 ggagactaca taaagtagtt ggtgggtgat gacgggatcg aaccgccgac cccctccttg
    27361 taagggaggt gctctcccag ctgagctaat caccccgctg tgtggagtcg cattataggg
    27421 agagttcaaa atgagtcaac gcattttcta aagaaattgt tcgttcgtcg taaatttaag
    27481 caagatgatc gcaaaacaga ccgtgttgcg caatttgtca acgaaaacaa taatgcgtaa
    27541 ggtagaaacc cgaactacat tgaggaatca ggcgggagtg atagaatatc gcccactcaa
    27601 tttttccagg atttgccggt tgtcggcatc tttctaaacg taaggccatt tcatgaaaat
    27661 caaaactcgc ttcgcgccaa gcccaacagg ctatctgcac gttggcggcg cgcgtactgc
    27721 tctttactcc tggctttttg cacgtaacca cggcggtgag ttcgtgctgc gtattgaaga
    27781 caccgatctt gagcgttcca cgccggaagc tatcgaagcc attatggatg gcatgaactg
    27841 gctgagcctg gagtgggatg aaggtccgta ctaccagacc aaacgttttg atcgctacaa
    27901 tgcggtgatc gatcagatgc tggaagaggg cactgcttat aaatgctatt gctctaaaga
    27961 gcgcctggaa gcgctgcgcg aagagcaaat ggcgaaaggt gagaagccgc gttatgacgg
    28021 tcgctgccgc cacagccatg agcatcatgc tgatgatgaa ccgtgtgtcg tgcgttttgc
    28081 taatccgcag gaaggttctg ttgtttttga cgatcagatc cgtggtccga tcgagttcag
    28141 caaccaggag ctggatgatc tgattatccg ccgtaccgat ggttccccaa cctataactt
    28201 ctgtgtggtt gtcgatgact gggatatgga aatcacccac gttatccgtg gcgaagacca
    28261 tatcaacaac acgccgcgcc agatcaacat tcttaaggcc ctgaaagcgc cggtgccggt
    28321 ttacgcgcac gtttctatga tcaatggcga tgacggtaaa aaactgtcca aacgtcacgg
    28381 ggcagtcagc gtaatgcagt atcgtgatga cggttatttg ccagaagcac tgctgaacta
    28441 tctggtgcgt ctgggctggt cccacggcga tcaggaaatc ttcactcgtg aagagatgat
    28501 caaatacttc actttgaatg ccgtcagcaa atctgccagt gcgttcaaca ccgacaagct
    28561 gctgtggctg aaccatcact acattaacgc gctgccgccg gagtatgttg ctactcactt
    28621 acagtggcac attgagcagg aaaatatcga tacccgtaac ggcccgcagc tggctgatct
    28681 ggtgaaactg ctgggcgaac gctgcaagac gctgaaagag atggcacaga gctgccgtta
    28741 tttctacgaa gattttgctg agttcgatgc cgatgcggcg aaaaaacatc tgcgtccggt
    28801 agcgcgtcag ccgctggaag tggttcgtga caaactggcc gcgattactg actggaccgc
    28861 tgaaaacgtt catcacgcta ttcaggcgac ggcggatgag ctggaagtgg gtatgggtaa
    28921 agttggtatg ccgctgcgtg tcgccgtaac cggtgcgggg cagtctccag cactggatgt
    28981 taccgttcac gcaattggta agacccgcag tatcgagcgt atcaacaaag cgctggattt
    29041 tattgctgaa cgcgaaaatc agcagtaatt agcgtgtaaa aagataaacg gcaggagata
    29101 atatcctgcc gttttttatt tatgctgatt cgtcaatatc gagacgggcg aggaaattgc
    29161 gaattccttc acgtgacaaa aattgcgcga ctttttcctg ctcaatgtct gtcatattat
    29221 ctatcgcgtt gatgatttgt cgatacgccg gggcgcgtgc tccaggagcg tattcagcaa
    29281 ttctctcttc agcttccatc aacattggtg tgttatggaa ggctggcgtg ttctgaataa
    29341 actcgcaaac ttgcgtatcg accagaataa gtctggcacg accgcctttc acgccgggaa
    29401 atttttccgt tttccagcct ttttctctga tccaacggtt aacagtttgt ttagccacac
    29461 cgaggcattc agccagttct tcggtggtca ttttgttgcg taatcttttc atattatttg
    29521 ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc cttcccgcag aagcaaggat
    29581 gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca gtgtcaccag caatacttca
    29641 agcggcgcat caccagaaag ggcaggcgct tctccctgac cttctggacg ttcagcattg
    29701 cgaatatatt cacgaacctg ttcattgacg tgaaccagtc gggctttgcc accctggacg
    29761 ccaggttttg gtgacgttgt ccagccttcc ttgcgtaccc atttattaat ggtctggcgg
    29821 ctatagccgg tcagtctggc aagttcatct ggtatcatcc tctccttgaa cataacaatt
    29881 ccctgaatgt atgtgggtct attagtagtt cattttataa caccacttta caggaaaccg
    29941 tgacatgaat aactactgaa tgcgagcggt tttccaaaat tgttcatttg cctgattttt
    30001 cagcgattga aactacacgt tgcaatttgc cgttgacaca ttcgggcgga attcatatga
    30061 tgccgcccgt caactcgaca agctttacgt gacggggcta tagctcagct gggagagcgc
    30121 ttgcatggca tgcaagaggt cagcggttcg atcccgctta gctccaccaa attttgcacc
    30181 cagcaaattt ggtacgtaaa cgcatcgtgg ggctatagct cagctgggag agcgcttgca
    30241 tggcatgcaa gaggtcagcg gttcgatccc gcttagctcc accaaatttc caaccctcgc
    30301 tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt tgtaatacag tctacgtccg
    30361 ggttagcgcc gcctggtgaa agcatcattg gatgaaaaat cggcaacagg ctggcccctg
    30421 tttgcttcgc gatgcgaata aacttattat ttgtgtgcct gaaaaccccg atcagtgaga
    30481 gtagtgtact catgtttgtg gagcataacc tgataaaaaa tatcaagata ttcacactag
    30541 cgtttacgct caccgtggta cttattcagc tatcccgttt tatttcgcca cttgccatta
    30601 tccattccag ttatatcttt ctggcgtgga tgccactgtg cgtaatgctg tcaatcttgt
    30661 ttatctttgg ctggcgcggt gtcgttcccg ttttatgcgg gatgttttgc accaatctgt
    30721 ggaactttca tctctctttt ttacagactg cggtcatgct cggtagccag acgtttgtcg
    30781 tgttgtgtgc ctgcgcaata ttacgctggc agctggggac gcgttggcgt tatggattga
    30841 ccagccgata tgtctggcaa cgtctgttct ggcttggttt ggtgacgccg atcggcatca
    30901 aatgcagcat gtatcttgtg gggagcttct ttgatttccc gctaaagata tccacctttt
    30961 tcggcgatgc ggatgccatt ttcacggtcg ttgatttgct aagccttttc accgctgtgc
    31021 tgatttacaa catgcttttc tactatctca ctcgcatgat tgtaagtccc cactttgcgc
    31081 agatattgtg gcgcagggat atcgctccgt cgttgggcaa agagaaacgc gcatttacct
    31141 taagctggct ggcagcgtta agcgtgctgc tacttctgtt gtgcacacct tatgaaaacg
    31201 actttattgc cggttacttg gtacccgttt tcttcatcat ctttaccctc ggggtcggta
    31261 agcttcgcta tccgttttta aatctcacct gggctgtttc aacgctttgc ctgctgaatt
    31321 acaaccagaa ctttttgcaa ggggtggaaa ccgaatattc gctggcattt attcttgcgg
    31381 tgctgatttc ctttagcgtt tgcctgctct atatggtgcg catttatcat cgcagtgaat
    31441 ggcttaatcg ccgctggcat ttgcaggcgc tgacagatcc gttaacgctc ctacccaact
    31501 ttcgtgcgtt ggaacaagcg ccggaacaag aggcgggcaa gagtttttgc tgcctgcgca
    31561 ttgataatct tgagtttatg agtcgtcatt acggcttaat gatgcgcgtt cactgtatcc
    31621 gctcaatttg ccgtacgctg ctgccgttga tgcaggaaaa cgaaaagttg tatcaattgc
    31681 cgggtagtga actgctgtta gtgctgagcg ggccggaaac ggaagggcga ctccagcata
    31741 tggttaacat cctgaatagt cggcaaattc actggaacaa taccgggctg gatatgggct
    31801 atggtgctgc ctgggggcgt tttgatggaa atcaggaaac cctgcaaccc ttgttggggc
    31861 agttaagctg gctggcggag caatcctgcg cacatcatca tgtgctggcg ctggatagca
    31921 gagaggagat ggtttccggg cagaccacta aacaggtgct attgctgaat accattcgca
    31981 cggcgttaga tcagggtgat ttgctgctct acgcccagcc aattcgcaac aaagagggtg
    32041 aaggttatga tgagatcctc gcgcgactga aatatgacgg cggcattatg accccggata
    32101 agtttctgcc ccttattgct cagtttaacc ttagcgcgcg ttttgatttg caagtgctgg
    32161 aatccttgtt gaagtggctg gcaacacacc cttgcgacaa aaaaggaccg cgcttttcag
    32221 tcaatttaat gccgctcacg ctgctgcaaa agaatattgc cgggcggatt attcgtctgt
    32281 ttaagcgtta tcacatctcc ccgcagtcgg tcattcttga gatcaccgag gagcaggcgt
    32341 tttctaacgc agaaagcagc atgtacaaca tcgagcagct gcataagttt ggtttccgga
    32401 ttgcgattga tgactttggc accggatatg ccaactacga acggttaaag cgtttgcagg
    32461 ctgatatcat caaaattgat ggcgtctttg tgaaagatat tgtcacgaac acgctggatg
    32521 cgatgattgt gagatcaatt accgatctgg cgaaagcgaa gtcattgagt gtggtcgcgg
    32581 agtttgtcga gacgcaacag cagcaggcgc tattgcataa gctcggggtg caatatctgc
    32641 aagggtattt gattggtcgc ccgcagccat tagctgatta acaggcgtaa aaaagccggg
    32701 gaatgatccc cggctttttt atgtatggtt ttacagcacc agtgctgcga ttgacgcaga
    32761 cagcacactc accagggtag agccgtaaac cagtttcaga ccgaagcgag aaaccacgtt
    32821 accttgctct tcattcaggc ctttaactgc acctgcgata atcccgattg aagagaagtt
    32881 agcgaaggaa accaggaaca cagagatgat gccttcagca cgcggagaga gcgtggaagc
    32941 aattttctgc agatccatca tcgcaacgaa ctcgttggaa accagtttgg tcgccatgat
    33001 actgcccact tgcagtgctt cactggaagg aacacccatc acccatgcaa tcggatagaa
    33061 gatgtagccc aggatgccct ggaaggagat gctgtagcca aaccagccag tcacagtagc
    33121 aaacagtgcg ttcagtgcgg cgatcagggc gataaagccg atcagcatcg cggcaacgat
    33181 aatggcaacc ttgaaacctg ccagaatgta ttcacccagc atttcgaaga agctctgacc
    33241 ttcgtgcagg ttggacatct gaatgttttc ttcactggca tcaacacggt aagggttgat
    33301 cagcgacagc acgataaagg tgctgaacat gttcagtacc agcgccgcaa cgacatattt
    33361 cggctccagc atggtcatgt atgcaccaac gatggacata gatacggtgg acatcgccgt
    33421 tgctgccatg gtgtacatac gattgcggga gattttgccg aggatatctt tataggcaat
    33481 aaagttttca gactgaccca gaatcaggga gctgacggcg ttaaaggatt ccagtttgcc
    33541 catgccgttg actttggaga gcaggaaacc aattgcgcgg ataatcaccg gcaacacgcg
    33601 aatgtgctgg agaataccga tcagtgcaga gataaagacg attgggcaca gcactttcag
    33661 gaagaagaat gccaggcctt gatcattcat gctaccaaag acgaagttag tcccttcgtt
    33721 ggcaaatccg agcagttttt cgaacatttc ggagaagcct ttcacgaagc ctaaaccaac
    33781 gtcggagttc aggaagaacc acgccagtaa cacttcgata acaagcagtt gaataacata
    33841 acggatacga atttttttgc ggtcgctgct taccagcagt gcgagaatcg caacaacggc
    33901 aagtgccagt acaaaatgaa ggacgcggtc catatttgct ccaaatatga ggcaggttaa
    33961 atttccgtgc acattctatg taacatggtt aaggaaaacg agatctgaca cacactataa
    34021 tgacgtcctg tgacaagctt caaaaatagt gatctgtcat acatttctgc tatgttgtgt
    34081 atggaagctg aaagttatgt aaatgtgcta acattaaaca taaccataac attcaagtca
    34141 tcaatctttt cgtagtcata atccaggtct atcagagaaa tcaccacaac ccatttaaat
    34201 gaatttgata atcattctcg tttggcatag catgaaacat agcaaaggct atgttttaga
    34261 ggcacaagat gacgaactat cgcgttgaga gtagcagcgg acgggcggcg cgcaagatga
    34321 ggctcgcatt aatgggacct gcgttcattg cggcgattgg ttatatcgat cccggtaact
    34381 ttgcgaccaa tattcaggcg ggtgccagct tcggctatca gctactgtgg gttgtcgttt
    34441 gggccaacct gatggcgatg ctgattcaga tcctctctgc caaactaggg attgccaccg
    34501 gtaaaaatct ggcggagcag attcgcgatc actatccgcg tcccgtagtg tggttctatt
    34561 gggttcaggc agaaattatt gcgatggcaa ccgacctggc ggaatttatt ggtgcggcga
    34621 tcggttttaa actcattctt ggtgtctcgt tgttgcaggg cgcggtgctg acggggatcg
    34681 cgactttcct gattttaatg ctgcaacgtc gcgggcaaaa accgctggaa aaagtgattg
    34741 gtgggttact gttgtttgtt gccgcggctt acattgtcga gttgattttt tcccagccta
    34801 acctggcgca gctgggtaaa ggaatggtga tcccgagttt acctacttcg gaagcggtct
    34861 tcctggcagc aggcgtgtta ggggcgacga ttatgccgca tgtgatttat ttgcactctt
    34921 cgctcactca gcatttacat ggcggttcgc gtcaacaacg ttattccgcc accaaatggg
    34981 atgtggctat cgccatgacg attgccggtt ttgtcaatct ggcgatgatg gctacagctg
    35041 cggcggcgtt ccacttttct ggtcatactg gtgttgccga tcttgatgag gcttatctga
    35101 cgctgcaacc gctgttaagc catgctgcgg caacggtctt tgggttaagt ctggttgctg
    35161 ccggactgtc ctcaacg
//
LOCUS       DAZFVO020000031        35163 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFVO020000031 DAZFVO020000000
VERSION     DAZFVO020000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.031"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..396)
                     /locus_tag="ACHKNB_RS07275"
                     /old_locus_tag="pgaptmp_001455"
     CDS             complement(<1..396)
                     /locus_tag="ACHKNB_RS07275"
                     /old_locus_tag="pgaptmp_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4194998.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVS"
     gene            complement(493..3774)
                     /gene="yphG"
                     /locus_tag="ACHKNB_RS07280"
                     /old_locus_tag="pgaptmp_001456"
     CDS             complement(493..3774)
                     /gene="yphG"
                     /locus_tag="ACHKNB_RS07280"
                     /old_locus_tag="pgaptmp_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HGU4194999.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3892..5085
                     /gene="yphH"
                     /locus_tag="ACHKNB_RS07285"
                     /old_locus_tag="pgaptmp_001457"
     CDS             3892..5085
                     /gene="yphH"
                     /locus_tag="ACHKNB_RS07285"
                     /old_locus_tag="pgaptmp_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGU4195000.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPKGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(5149..6402)
                     /gene="glyA"
                     /locus_tag="ACHKNB_RS07290"
                     /old_locus_tag="pgaptmp_001458"
     CDS             complement(5149..6402)
                     /gene="glyA"
                     /locus_tag="ACHKNB_RS07290"
                     /old_locus_tag="pgaptmp_001458"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGU4195001.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6730..7920
                     /gene="hmpA"
                     /locus_tag="ACHKNB_RS07295"
                     /old_locus_tag="pgaptmp_001459"
     CDS             6730..7920
                     /gene="hmpA"
                     /locus_tag="ACHKNB_RS07295"
                     /old_locus_tag="pgaptmp_001459"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HGU4195002.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7965..8303)
                     /gene="glnB"
                     /locus_tag="ACHKNB_RS07300"
                     /old_locus_tag="pgaptmp_001460"
     CDS             complement(7965..8303)
                     /gene="glnB"
                     /locus_tag="ACHKNB_RS07300"
                     /old_locus_tag="pgaptmp_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HGU4195003.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8364..9698)
                     /gene="glrR"
                     /locus_tag="ACHKNB_RS07305"
                     /old_locus_tag="pgaptmp_001461"
     CDS             complement(8364..9698)
                     /gene="glrR"
                     /locus_tag="ACHKNB_RS07305"
                     /old_locus_tag="pgaptmp_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HGU4195004.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9688..10401)
                     /gene="qseG"
                     /locus_tag="ACHKNB_RS07310"
                     /old_locus_tag="pgaptmp_001462"
     CDS             complement(9688..10401)
                     /gene="qseG"
                     /locus_tag="ACHKNB_RS07310"
                     /old_locus_tag="pgaptmp_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HGU4195005.1"
                     /translation="MRHIFQRLMPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(10566..11993)
                     /gene="qseE"
                     /locus_tag="ACHKNB_RS07315"
                     /old_locus_tag="pgaptmp_001463"
     CDS             complement(10566..11993)
                     /gene="qseE"
                     /locus_tag="ACHKNB_RS07315"
                     /old_locus_tag="pgaptmp_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HGU4195006.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12551..16438)
                     /gene="purL"
                     /locus_tag="ACHKNB_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /gene_synonym="purI"
     CDS             complement(12551..16438)
                     /gene="purL"
                     /locus_tag="ACHKNB_RS07320"
                     /old_locus_tag="pgaptmp_001464"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGU4195007.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKVLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16696..18252
                     /gene="mltF"
                     /locus_tag="ACHKNB_RS07325"
                     /old_locus_tag="pgaptmp_001465"
     CDS             16696..18252
                     /gene="mltF"
                     /locus_tag="ACHKNB_RS07325"
                     /old_locus_tag="pgaptmp_001465"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HGU4195008.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIANSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(18249..18752)
                     /gene="tadA"
                     /locus_tag="ACHKNB_RS07330"
                     /old_locus_tag="pgaptmp_001466"
     CDS             complement(18249..18752)
                     /gene="tadA"
                     /locus_tag="ACHKNB_RS07330"
                     /old_locus_tag="pgaptmp_001466"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGU4195009.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(18810..19445)
                     /gene="yfhb"
                     /locus_tag="ACHKNB_RS07335"
                     /old_locus_tag="pgaptmp_001467"
     CDS             complement(18810..19445)
                     /gene="yfhb"
                     /locus_tag="ACHKNB_RS07335"
                     /old_locus_tag="pgaptmp_001467"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HGU4195010.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19654..20502
                     /gene="yfhH"
                     /locus_tag="ACHKNB_RS07340"
                     /old_locus_tag="pgaptmp_001468"
     CDS             19654..20502
                     /gene="yfhH"
                     /locus_tag="ACHKNB_RS07340"
                     /old_locus_tag="pgaptmp_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGU4195011.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20558..20818
                     /gene="yfhL"
                     /locus_tag="ACHKNB_RS07345"
                     /old_locus_tag="pgaptmp_001469"
     CDS             20558..20818
                     /gene="yfhL"
                     /locus_tag="ACHKNB_RS07345"
                     /old_locus_tag="pgaptmp_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HGU4195012.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(21012..21092)
                     /gene="shoB"
                     /locus_tag="ACHKNB_RS07350"
                     /old_locus_tag="pgaptmp_001470"
     CDS             complement(21012..21092)
                     /gene="shoB"
                     /locus_tag="ACHKNB_RS07350"
                     /old_locus_tag="pgaptmp_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HGU4195013.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21512..21892)
                     /gene="acpS"
                     /locus_tag="ACHKNB_RS07355"
                     /old_locus_tag="pgaptmp_001471"
     CDS             complement(21512..21892)
                     /gene="acpS"
                     /locus_tag="ACHKNB_RS07355"
                     /old_locus_tag="pgaptmp_001471"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGU4195014.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21892..22623)
                     /gene="pdxJ"
                     /locus_tag="ACHKNB_RS07360"
                     /old_locus_tag="pgaptmp_001472"
     CDS             complement(21892..22623)
                     /gene="pdxJ"
                     /locus_tag="ACHKNB_RS07360"
                     /old_locus_tag="pgaptmp_001472"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HGU4195015.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22635..23363)
                     /gene="recO"
                     /locus_tag="ACHKNB_RS07365"
                     /old_locus_tag="pgaptmp_001473"
     CDS             complement(22635..23363)
                     /gene="recO"
                     /locus_tag="ACHKNB_RS07365"
                     /old_locus_tag="pgaptmp_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGU4195016.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(23375..24280)
                     /gene="era"
                     /locus_tag="ACHKNB_RS07370"
                     /old_locus_tag="pgaptmp_001474"
     CDS             complement(23375..24280)
                     /gene="era"
                     /locus_tag="ACHKNB_RS07370"
                     /old_locus_tag="pgaptmp_001474"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGU4195017.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTGGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(24277..24957)
                     /gene="rnc"
                     /locus_tag="ACHKNB_RS07375"
                     /old_locus_tag="pgaptmp_001475"
     CDS             complement(24277..24957)
                     /gene="rnc"
                     /locus_tag="ACHKNB_RS07375"
                     /old_locus_tag="pgaptmp_001475"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGU4195018.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(25230..26204)
                     /gene="lepB"
                     /locus_tag="ACHKNB_RS07380"
                     /old_locus_tag="pgaptmp_001476"
     CDS             complement(25230..26204)
                     /gene="lepB"
                     /locus_tag="ACHKNB_RS07380"
                     /old_locus_tag="pgaptmp_001476"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGU4195019.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(26220..28019)
                     /gene="lepA"
                     /locus_tag="ACHKNB_RS07385"
                     /old_locus_tag="pgaptmp_001477"
     CDS             complement(26220..28019)
                     /gene="lepA"
                     /locus_tag="ACHKNB_RS07385"
                     /old_locus_tag="pgaptmp_001477"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGU4195020.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(28217..28696)
                     /gene="rseC"
                     /locus_tag="ACHKNB_RS07390"
                     /old_locus_tag="pgaptmp_001478"
     CDS             complement(28217..28696)
                     /gene="rseC"
                     /locus_tag="ACHKNB_RS07390"
                     /old_locus_tag="pgaptmp_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HGU4195021.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28693..29649)
                     /gene="rseB"
                     /locus_tag="ACHKNB_RS07395"
                     /old_locus_tag="pgaptmp_001479"
     CDS             complement(28693..29649)
                     /gene="rseB"
                     /locus_tag="ACHKNB_RS07395"
                     /old_locus_tag="pgaptmp_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HGU4195022.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKHLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29649..30299)
                     /gene="rseA"
                     /locus_tag="ACHKNB_RS07400"
                     /old_locus_tag="pgaptmp_001480"
     CDS             complement(29649..30299)
                     /gene="rseA"
                     /locus_tag="ACHKNB_RS07400"
                     /old_locus_tag="pgaptmp_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HGU4195023.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(30332..30907)
                     /gene="rpoE"
                     /locus_tag="ACHKNB_RS07405"
                     /old_locus_tag="pgaptmp_001481"
     CDS             complement(30332..30907)
                     /gene="rpoE"
                     /locus_tag="ACHKNB_RS07405"
                     /old_locus_tag="pgaptmp_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HGU4195024.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30904..31059)
                     /gene="rseD"
                     /locus_tag="ACHKNB_RS07410"
                     /old_locus_tag="pgaptmp_001482"
     CDS             complement(30904..31059)
                     /gene="rseD"
                     /locus_tag="ACHKNB_RS07410"
                     /old_locus_tag="pgaptmp_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HGU4195025.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            31315..32937
                     /gene="nadB"
                     /locus_tag="ACHKNB_RS07415"
                     /old_locus_tag="pgaptmp_001483"
     CDS             31315..32937
                     /gene="nadB"
                     /locus_tag="ACHKNB_RS07415"
                     /old_locus_tag="pgaptmp_001483"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HGU4195026.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(32922..33659)
                     /gene="trmN"
                     /locus_tag="ACHKNB_RS07420"
                     /old_locus_tag="pgaptmp_001484"
     CDS             complement(32922..33659)
                     /gene="trmN"
                     /locus_tag="ACHKNB_RS07420"
                     /old_locus_tag="pgaptmp_001484"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HGU4195027.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            33791..35125
                     /gene="srmB"
                     /locus_tag="ACHKNB_RS07425"
                     /old_locus_tag="pgaptmp_001485"
     CDS             33791..35125
                     /gene="srmB"
                     /locus_tag="ACHKNB_RS07425"
                     /old_locus_tag="pgaptmp_001485"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HGU4195028.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         8431 a         9438 c         8914 g         8380 t
ORIGIN      
        1 cgagacatat ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa tcaccggaat
       61 acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca ctgccgataa
      121 aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt ctttcgaaat
      181 atcaccctgg gcgttagttt caattaactg tacctgtact gaagaatctt ttgccgcatc
      241 ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata ttgcaccaat
      301 ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg tacccattaa
      361 taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag ggtttttatt
      421 aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct caaatattac
      481 agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacggtgg tgaataatgc
      541 cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagatt catattgcgc
      601 aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt ctttatgctg
      661 ctgttgtaaa tcgccataca gcgacaacag gtcaggttgt gaaacagcaa agaaatccgc
      721 ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa acagttgctg
      781 tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga gataatcaac
      841 cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg ccgccagacg
      901 taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg cctgccagaa
      961 ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct ccggataatg
     1021 cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa gatgctgcca
     1081 ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc cgccttccca
     1141 cgggtggaat ttgcgcgtgg cgagaatgtc cgccgctttg tctgcctgac ccgtgagatg
     1201 ccacaaattg agcaactctg ctgtcatgtc gtcgcgtttc agcgcaattt ccagattatt
     1261 ttccagacgc gccagtcgtt tctccggtgt ggctccgctt aacttatcaa gcaaatcccg
     1321 ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa gataacgcgc
     1381 ggccagctca taatcgtgtt gcttattcca cgcatggatc gctaacccgc gccagccgtc
     1441 ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gtaatggctt tgttgtagct
     1501 acgcttgttg tagtagaagc aggccagtaa atgacgggca aaccagcact cttcaatact
     1561 ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg ggaagacatc
     1621 aatggctttt gcgaccagtt cgccgcgttc ggctttcggc aataaactcg cctgcaggta
     1681 aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca gggtgggcat
     1741 tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc cctgacacag
     1801 accgcgccac tgcgcgagag ctgattcgct acgaccatcg aaccagttca gccaccacag
     1861 agtggcgttc agcggataat cgcgcagcag tttctcgcgc tgcacacgcg cgttgtcctg
     1921 acgaccactt aacaccagca gcagattatg caggcaaagc acttcctgat tggttgggca
     1981 ggcgcgaaga ctttgttggc aaaaatccag accagcgtcg aagttaccat tacgcgccgc
     2041 cagtcgtgcc aggccataat agccaccggc tttgctgttg ccgctccaga ccgcacgcca
     2101 gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg cgcgaatcaa
     2161 actggcctgt ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca gcgcctgagt
     2221 ggcatacgcc accgcttgcg ggaaatcggc gcggttatat tccagcatcg ccagcgccag
     2281 gttacagcgg taatccagcg gatccagcac cacgccgcgc aggtagtaat cgaacggtga
     2341 acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt catctgtact
     2401 ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca actcttgcgg
     2461 ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagct caatggtcag
     2521 cctttcagga ttgataccgt gcaacacgcc ctggatggcg gtcgcaggca tcagtgccac
     2581 ggcatcatca agtaacgcgt tgcatttgcc gatttcgcgg atcgccaggc ggtatccgtt
     2641 caacggagag atggcataca gcccccactc aatcccccgc ttactacgct ggagttttat
     2701 caccgcatcg cgggaggcat tttgcaccat gcccaaagaa tgataaggca ggaaatactg
     2761 ctcaaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcgggctggt tatcggcaaa
     2821 aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct tatcccacgc
     2881 ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcga tatggtggtt
     2941 ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa aatcgtactg
     3001 cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag aaatgtccac
     3061 tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag cggagacggc
     3121 ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct gatgcccttc
     3181 cccccctttc actgccgggt tgacccacca caagaaatga cgcggcgtgg cgttaccgtt
     3241 atagacgcgg ctggcgattt ccagcgccgc ccggtcaggg cgcagggtga aacctgtcat
     3301 cacctgtaaa ccatgcatcg gctccgtttc gcctacccac accgtctgtg caccgtcttc
     3361 atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatgat gttgcggcca
     3421 gttaaactca atcccaccgg aaatccacgg ccccagcagc cccaccagcg caggtttaat
     3481 gacttcattg tgataaacaa aatcgcgctg tttcacttta tcccacgcgc gatgcacccg
     3541 accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc acaccgcctg
     3601 ccaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg gataaaccgc
     3661 gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat cctgcggccc
     3721 ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag tcatggtgcc
     3781 ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg aacaatctgg
     3841 caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa gatgagagcc
     3901 tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga actgctgtac
     3961 cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cacaaatttc gattccggca
     4021 gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat tgacgatgaa
     4081 agccagacgc gcgggcatag tagcggtaca tggctgattg cgccgaaagg tgactggacg
     4141 ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc ttgtttaagt
     4201 ccgaaaggtg aatttgagta tttacagatt gatgcaccga cgccgcaggc gctgctgtcc
     4261 gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac catcaatctg
     4321 gcgctggcaa tccacggtca ggttgatccg gtgaccggcg tgtcgcaaac catgccgcaa
     4381 gcgccgtgga caacaccggt tgaggtgaag tatctgctgg aagagaagct gggcattcgg
     4441 gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca aaataattcg
     4501 caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc gttcgtgatt
     4561 aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca caccatcgtt
     4621 aatccggatg gcgtcgtctg cgactgtgga cgttacggct gcctggaaac cgtcgcctcg
     4681 ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt taatactcag
     4741 cttgatcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag tggagaaccg
     4801 tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct gtataacttc
     4861 ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc ctttggtgag
     4921 aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg cgacgaagga
     4981 ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca acaggtgctg
     5041 ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg cgataacgta
     5101 gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt atgcgtaaac
     5161 cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa cggcttcatc
     5221 attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt ctgcttcttt
     5281 gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca caaacgggct
     5341 cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc ccagagcggc
     5401 gtctgcttct ttaccggtca ggtttttatc aaccagatca accaggaaca ggtggttatc
     5461 agtgccgccg gaaaccactt tgtagccgcg ctcgaggaac acttctacca tcgctttagc
     5521 gtttttcgcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt ctttcagagc
     5581 aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc
     5641 agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca ggccgccgcg
     5701 cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag gaaccgggtt
     5761 cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga acaggtaagc
     5821 accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg aatatgcaga
     5881 gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca gatcggcgta
     5941 gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt taccggagaa
     6001 gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac ccagaacggt
     6061 atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg
     6121 ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg ccagttgttc
     6181 aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac cttcagcata
     6241 tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt agttttcgga
     6301 ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag cctgccacag
     6361 ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct cctgactcag
     6421 ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg aacagtataa
     6481 ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga ttaccttcag
     6541 gctacacaag gctttggaga ataaagagct tgcaaccgga aacggatttc ttttcaggtt
     6601 tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag aaccatttac
     6661 attgcagggc tattttttat aagatgcatt tgagatacat caattaagat gcaaaaaaag
     6721 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
     6781 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
     6841 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
     6901 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
     6961 gtagaaaaaa tcgcgcagaa gcacaccagt ttccagatca aaccggaaca gtacaacatc
     7021 gtcggcgaac acctgttggc gacgctggac gaaatgttca gcccgggcca ggaagtgctg
     7081 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
     7141 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
     7201 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
     7261 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
     7321 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
     7381 cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc
     7441 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
     7501 gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat gctggcaatg
     7561 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
     7621 aatggcgatg ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc
     7681 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
     7741 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
     7801 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
     7861 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa
     7921 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
     7981 tcctcttcac cggtacggat gcgaatgacc cgtgccacgt cgaagacgaa gattttaccg
     8041 tcaccgattt tgccggtttg cgccgtgcga ataatggtat caacacaggt atcgacaatg
     8101 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
     8161 ccgcggtaca gctcggtatg gcctttctgg cggccaaaac ctttcacctc agtgaccgtc
     8221 atgccggtaa tgccgacttc agccagtgct tcgcgaacat cgtccagctt gaagggttta
     8281 ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc
     8341 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
     8401 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgtgtgacg
     8461 ttgcctttgg tgatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc
     8521 tcaacaaagg ttggcagcgc cgtattttca ccctccagcg cctgctctac taatgcatcg
     8581 ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
     8641 acattacccg gccagctagc ggtcatcagg cgtttcatcg catcagtgga gaacgcgcgg
     8701 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag aagcggaatg
     8761 tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag gcggtaatag
     8821 aggtcttcac ggaattcccc gcgcgccatc gcttttggca ggtcgcggtg agtggcggaa
     8881 ataatccgca cgtcgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
     8941 tgcaacacgc gcagcagttt gacctgcaac ggtgcgggca tatcgccaat ctcatcgaga
     9001 aatagcgtac cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
     9061 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca
     9121 cagttaatag caataaatgg tttgctattg cgcgggctgg cgttgtggat agcctgggcg
     9181 aaaatctctt tcccggtgcc gctctggccg ttaatcaaaa cgctaacgtc tgattgcgcc
     9241 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aatcgcctcg
     9301 cgccagcgtt catcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat
     9361 agcgcatctt tatcgacagg cttggtgagg aaactgaaca cgccctgctg tgttgcagca
     9421 acggcatcgg gaatagaacc atgcgcggta agaataatta ctggcattcc cggctgcact
     9481 ttctgaattt cagcaaacag ctgcataccg tccatttcat ccatccgcag gtcgctgatg
     9541 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
     9601 accacgctgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
     9661 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
     9721 tggcgcgggc ttttcaccct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
     9781 ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg tgagttccag
     9841 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggcctg
     9901 ctggagcttg ccgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
     9961 gcgccagagt tgatacagag ggcgaacctg tgccgggatc tcggtactta gcgcatcaat
    10021 gcgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
    10081 ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgtgactg
    10141 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    10201 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    10261 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgactctg
    10321 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcattaa
    10381 tcgttgaaaa atgtgtcgca tattcaccag acttaatgcc tatcccagtg ggcgtaatta
    10441 ttgcaggcag tttggacatg gacagcgcgg agaaaccgga gcgtacacgt cgtacgtgag
    10501 gatttcgagc actgcccggg gccgaactgg caaataaaat agcctgatgg gataggctgt
    10561 tagcattatt tcgtgttttt cgacgacggc aattcaatgc ggaaacaaac gtcttgcccg
    10621 ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt
    10681 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccttg aaaaaagggt
    10741 tcgaagatca tggcgcgttc ctcttgcgga atgggtgtgc ctgtattgat gacatcaata
    10801 taaacccgtg caccatgtaa gctgctgcga aggcaaatgt taccggattc agccccgtag
    10861 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
    10921 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
    10981 tgagcagaaa ctactgtctc caccagcggt gctaactcaa cattctccag ttccaccgca
    11041 ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcgg
    11101 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc
    11161 tggtcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga
    11221 gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac
    11281 caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga aacgctattg
    11341 cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg
    11401 ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat
    11461 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
    11521 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga
    11581 cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc
    11641 aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
    11701 agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
    11761 agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca
    11821 ctgcgccggg cgtcaataag cgtggtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    11881 ttcaggcttt gccaggcttg ccatgccagc accaacaagg gcagcagaat tagcaaaaat
    11941 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    12001 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    12061 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    12121 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    12181 gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta
    12241 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt
    12301 gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    12361 aaagagacaa cttaagataa acttattaga ttacaataaa atcaatcatt taagtgtcgc
    12421 cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg
    12481 agttgggagc ttatgatagt ggttggtgct taatgccgca tccgggctgc aaaaccaatg
    12541 aactgatgaa ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat
    12601 cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt
    12661 gcggcatcat aatggtgact cgaccacttt cagtcgtgac tgccgtaata ccgttcggtg
    12721 aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg acatagcgca
    12781 gtgccaccag cactttgctt tccagtgccg ccagatgcgc cgcatcacgc acttccacgc
    12841 gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacagca
    12901 gagacgggct ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtat
    12961 tgcgcacaaa acgtggccac aactcactac ctgggatcag ttcgcgcagg ttagacatca
    13021 tctggcaacc gttacatacc cccagcgcca gcgtttgcgg acggtggaag aaggttgcaa
    13081 actcatcgcg tacacggtca ttgaacagga ttgacttcgc ccatccttca ccggcaccca
    13141 gcacatcacc gtaggagaaa ccaccgcacg cgaccagggc gtggaaatct tccaggcccg
    13201 tgcgtccagc cagcaggtcg ctcatatgca cgtcgatagc atcaaagcct gcacggtgga
    13261 aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagtacg gcaactttcg
    13321 gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg aacgacagtt
    13381 ttacattcag gcccggatcg gcgtcgttag atttcgcctg atgctcctga tcggcacact
    13441 ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg
    13501 tggtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg
    13561 aaacagcctg ccctacataa tggacacaat cagcaagccc atgctgtgcc agtacggact
    13621 cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc acccagttct tcgttaaaca
    13681 acgccgccag gcgatcgtcg cccagagagg caatatccgc gttaatgccg caatgaccag
    13741 caaaggccat ttccgccagc gttaccagca ggccgccatc agagcggtcg tggtatgcga
    13801 gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttgcgcga
    13861 cgtcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg
    13921 cgcccagcgc gttattgcct ttgcccaaat caatcagcag cagtgcgtta tcttcggtag
    13981 aaagctgtgg cgtgatggtg tgacgtacat cttccacgcg ggcaaatgcg gaaatcacca
    14041 gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg
    14101 acatagagtc tttacccacc gggatcgtaa gacccagcgc cggacaaagc tcttcaccca
    14161 cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc
    14221 agttggcgga aagtttaatg cgtttgatat caccaatctg tgttgcggca atattggtta
    14281 acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac
    14341 gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc
    14401 agttagcgac cggcacctgc cacggcccca ccatctgatc gcgcgctacc atgccggtta
    14461 cgctgcggtc gccaatggtc accaggaagg ttttttccgc cacagtcggc agatgcagca
    14521 cacgtttcac cgcatctgca atggtgatcc cttcacgggc cagcgcgtcg cctttcgctt
    14581 tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg tccagcggca
    14641 gatcgatcgg ctgattatca aaatgacgat cgtgcagaga aagatgcagt tcttcggtcg
    14701 cttcaccaat caccgcgtag ggtgcgcgtt cacgcttaca cagttcgtca aacagcggta
    14761 attgatcggc agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca
    14821 gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag ttcaaattta ccgccgcgtc
    14881 cgccgtcgct caccagctcc ggcatcgcgt tagaaagacc gcccgcaccg acgtcgtgga
    14941 taaacagaat tgggttagca tcaccaagct gccagcaacg gtcgatcact tcctggcagc
    15001 gacgctccat ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact
    15061 gacctgacgc catggaagac gccgctccgc cgccaagacc gatgttcatt gccgggccgc
    15121 cgagaacgac cagcttcgca ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa
    15181 tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccgt
    15241 tgtggctgtt cactttttct tcataagtac ggaagtagcc attcagtgcc ggacgaccaa
    15301 attcgttgtt aaacgccgcg ccgcccagcg ggccttcggt catgatgtcc agcgcggtga
    15361 caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagcca ggaattcgca
    15421 ggttggaaac ggagaaacca accaaacccg ctttcggctt cgcgccgcgc ccggtggcac
    15481 cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg
    15541 tcgggtggtt gtgagtttcg actttcatca gaatatgcgc cggttcctga tggaaatcgt
    15601 agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc attacggcgg
    15661 cgttatcttt ataagcagag agaacgtaat ctggcgtggt ttcgaaggtg tttttgatca
    15721 tcttaaacag cgatttcggc tgttgctcac cgtcgataac ccaatcggcg ttaaaaatct
    15781 tgtggcggca gtgctcggag ttcgcctggg cgaacatata cagctcgatg tcgttcgggt
    15841 tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc gccagtgcca
    15901 ggccaagacg caggttagcg tcgattagcg cctgacggcc ctgccccagc aaatcaacgc
    15961 tggtgaccgg agtcggttga tggtgtgcaa acagctgctc tgcatcatct aaagcaaaaa
    16021 agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg
    16081 tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta
    16141 gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac
    16201 gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc gggccatatt
    16261 tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa
    16321 aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga aaacgtgcca
    16381 gcagtttgtt gattcggaat gccgacagtg caggcgaacc acgcagaatt tccatcataa
    16441 gtctctcgtc ttctaagctt tcggtgtacc caaggggggg aaacgggcgt cattataaag
    16501 aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa
    16561 cgcgtgccgc caatggctgt aataagttgc catctggcgc aggtttacgc aaaatgccgc
    16621 tcatttatga gtaaaccttt cactattatt acgttttttc aagctgggac gcgcacgaca
    16681 cagagaatta actaattgaa aaaattaaag attaattatc tgttcatcgg cattctggca
    16741 ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc cgacaaccgt
    16801 atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac tcccctgact
    16861 tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa acagtttgcc
    16921 gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca gctgtttgac
    16981 gaccttgata atggtaacgc cgacctgctg gcggcaggac tggtctataa cagtgagcgg
    17041 gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact ggtttataaa
    17101 gtgggtcagt atcgcccacg tacgctgggc aacctgacgg cggagcaact caccgttgca
    17161 ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt cccggaatta
    17221 agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt catcgaagga
    17281 aaactcgatt acaccattgc taattctgtc gccatcagcc tgtttcagcg cgttcacccg
    17341 gagctcgccg tagcgctcga tatcaccgat gaacaaccgg tgacttggtt tagcccgtta
    17401 gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat gaatgaagac
    17461 ggtacgctgg cacgcattga agagaaatac ctggggcatg gcgatgattt tgattacgtc
    17521 gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt
    17581 gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta tcaggaatcg
    17641 cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa
    17701 aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag catcagcggt
    17761 ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgag
    17821 cggatctggt ttgccctcgc tgcgtacaat atgggctatg cgcatatgct ggatgcccgc
    17881 gccctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa acagcgtctg
    17941 cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc tcgtggacat
    18001 gaagcctacg cttatgtcga aaatattcgt aagtatcaga ttagcctggt gggttatctg
    18061 caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga ttatccggcg
    18121 gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct ttcccagtcg
    18181 tcatcaaact atttgaccca ttctccctct ctgctgtttt ccaggaaagg gagtgaagag
    18241 aaacaaaatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc ctggcggcgc
    18301 atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc ttccgtaatt
    18361 tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc cgcagcgcca
    18421 gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat catcgctccg
    18481 gcacacatta cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag acgataattt
    18541 tgcatcacca gaccaccctg ccgcagggcc atgatttctg catgtgcggt gggatcatgg
    18601 cgaccaatcg ggcggttcca gccttcgccg attacccgat tgttatgcac taataccgcg
    18661 ccgaccggca cttcccgctc atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc
    18721 cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg
    18781 cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc accgcgcggg
    18841 gttactcgcc aacgatgctg acagaaataa agcagcggat tgtcctgatt actgtcgcta
    18901 tagccactgt acagccgcag cggagtgccg attttgcgct ccagttgtgc gaccttttca
    18961 tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat ttggctggcg
    19021 ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc
    19081 ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt ggttaatcgc
    19141 tcctgaacca gcggaaaggc ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc
    19201 aacgtctgta aacgtgcttc gctgtgacca aaagtgcacc cccacagaag cagactcatc
    19261 ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg taacaacggc
    19321 aggacaagta acgcattcag cggttggcga cgcagtaaat agcgcagaaa actgccgaac
    19381 atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt
    19441 gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact acatagccta
    19501 acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac tggaattttt
    19561 aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtctc tcatattgcc
    19621 tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag
    19681 cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct gctacaacct
    19741 gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt cagtcagtcc
    19801 agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct taagttggcg
    19861 ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca taaccaaatc
    19921 cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa tactgccgct
    19981 atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt aacaatgttg
    20041 cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt ggcgcaaaac
    20101 tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga tatgcatgcg
    20161 ctactcgcaa cagtacaggc gtcgtcccct gacgatctgt tattagccat ttcctacacc
    20221 ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg cggaaaagtg
    20281 ctggcgatta ccggctttac tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat
    20341 accattgccg aagaacaagc gacaaacagc gcttcaatct ctgcttgtca cgctcaggga
    20401 atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact ggcaccagaa
    20461 cgtattcgtc atagtgaagc gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata
    20521 atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgttgttaa tcactaaaaa
    20581 atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt caatgggaga
    20641 tcatatctac gagattaaca gcgataagtg taccgaatgc gtagggcact acgagacacc
    20701 aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaagatccgg cgcatgtcga
    20761 gacagaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata aaatttaata
    20821 ttctactctg gaagtagagt attaattata ttactgggaa gccttaacgc cattatattt
    20881 atttaattga tgacattagc ataatcattc actaagttaa tttatatagt atctgccaag
    20941 acacttattt atagttatta aaggcgcgtc cgattggttc accggacgca ccttaagtac
    21001 gtttccttgt gttataagaa cagaaggatc agctgtaaaa cagcaatgat gattttgatg
    21061 acccgtttaa tcaggtatcg gcaatcagtc attcgttttt ccttaaacaa ggaatgcagc
    21121 cgttcagatt agcccttaca tctccccaaa actgaacgtg cgagttattg agggtgcatg
    21181 ctgcactcca caccagagct ttgacgacac cactcgtttc aatgggggaa ttctgtggca
    21241 tggtgtaaag cacagcaaaa tcttcaataa cgaagccaat tttaatgtac ttacgaattg
    21301 gcggtcacgt taatctttcc atcaatattg ctttcttcgt aaaggctcga gtttttatgc
    21361 taaagattgc aagttgcttg taaaagataa gtacactgat ccataatcgc tgttgttgag
    21421 ggtgcatgct gcacaaaatt aaagttaaaa agtaaaaccc ccgttcctta ccagttcggg
    21481 ggttttactt tttaaagaga acggtattat tttaactttc aataattacc gtggcacaag
    21541 catagtggcg ttcatctgcc agcgttacat gcatatttac aacgccgagc ttttccgcca
    21601 gttttaatgc ctcaccccat agccgtagcc gtggtttgcc gagctcatca ttgaatactt
    21661 caaattgatt aaacgccaga ccattgcgga tcccggtgcc aaacgctttt gctgcggctt
    21721 ctttcacagc aaaacgcttc gccagaaaac gcaccggctg gtggtgcgtt ttccagattg
    21781 cccattcgtt atcgcttaat acgcggcgtg ccaggcgctc accggatcgg gcgatcactg
    21841 cttctatgcg agcgatctct acgatatccg tgcctaaacc taatattgcc attagccacg
    21901 cgcttccagc atcagacgct tcatttctgc caccgcatct ttcagtccgg tcatcactgc
    21961 acgaccaata atggcatgac cgatattcag ttcatgcatc tcagggatgg cggcaatagc
    22021 tttcacgttg tgataggtca gaccgtgacc agcgttaact ttcagaccga gactagcggc
    22081 aaaggtcgcg gctttggcga tacgcgccag ctcttgcgcc tgttcggcgt cagttttggc
    22141 atcagcatag caaccggtgt ggatctcgat aaacggtgcg ccaacctctg ccgcagcttt
    22201 gatctgctct tcatcggcgt cgataaagag agaaacctgg atcccggcat ctgccagacg
    22261 tttgcaggca tcgcgcattt tgtcacgctg ccctgcgacg tccaggccgc cttcggttgt
    22321 tacttcctga cgcttttccg gtaccagaca gcaaaaatgt ggcttcgtct caacggcgat
    22381 cgccagcatc tcttcggtca ccgccatctc cagattcatg cgggtatcca gcgtctgacg
    22441 caggatgcgc acatcacggt cagtaatgtg gcggcgatct tcacgtaaat gcacggtaat
    22501 gccgtccgct cccgcctgct cagcaataaa cgccgcctgc accggatccg ggtaagcagt
    22561 accgcgtgcg ttacgcagcg ttgcaatatg gtcaatgttg acgcctaaca gtaattcagc
    22621 catgacaatc ctcatcattc ataatgtgtt ttcaccgttc gcttaggcat aaactgccgg
    22681 aacagttccc tgctctttaa tggtttaccg ccaagatacg gcttaagcgc catgcgggta
    22741 aagcgtttcg cggcgcgtag tgtgtctgcg tcaggaaatt cccgcgcgtt taacgctttt
    22801 aactgccttc cggtgaacgt tttattgtcg ataacgacgc ttgcgataaa ccctttttct
    22861 tcgcgataac gatacgtcat ggtgtcatct accggctcgc cgctacccgc acaatgggta
    22921 aaattgacgc catagcccag atgcccgagc agtgccaatt caaagcggcg cagcgcgggt
    22981 tctggcgtac cagtggcacc tgcaagagac tgaatgcagt gcaagtaatc gaaaaagagt
    23041 tcagagaagc gcgtctcgta ttccagtacg cgggagagaa gttcgttgat gtacagaccg
    23101 ctgtaaagcg tgataccgct taatggcagc gccagcgaga cagcttcagc actgcgcagc
    23161 gttttgactt cgccacgccc gccgaaacgt agcaagagag gggtgaaagg ctgtaatgca
    23221 cctttcagag tagagcgttt agagcgtgcg cctttggcaa ccagacgcac gcgacccgat
    23281 tcctccgtga agacgtccag catcaggctg gtttcgctcc acgggcgact atgcaggaca
    23341 aatgcgcgct gccagccttc catcggagtg actcttaaag atcgtcaacg taaccgagac
    23401 tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac ttttacccac agttcaaggt
    23461 ggacaggcgc ttcgaacatt tcctgcatgt ctttacgcgc ttcaatcccg atggttttga
    23521 tcttggcccc tttgttgcca ataaccatct tcttctgccc ttcacgctca acgagaatca
    23581 aaccattgat gtcataacca ccgcgttcgt tagagacgaa acgttcgatc tccacggtca
    23641 cggagtacgg cagttcagcg ccgaggaaac gcatcagttt ttcgcggatg atttcagacg
    23701 ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg gaagtgatgg gtcgcttcag
    23761 ggaggtgctt acgcacgatt gctgcgatag tgtcgacatt cagcccggtt tcagcagaga
    23821 tcggcacgat atcgaggaag ttcatctgac ttgccaggaa ctgtaagtgc ggcagcagat
    23881 cggctttttc ctgcacgtta tccactttgt tcaccgcgag gatcaccggc gctttgccat
    23941 cgcgcagttt gttgagcacc atttcgtcgt ccggcgtcca gcgggtgcct tcaacgacaa
    24001 aaatcaccag ctcaacgtcg ccaatagagc tgctcgccgc tttgttcatc aggcggttaa
    24061 tagcgcgttt ttcttccata tgcaggccag gggtatcaac gtagatcgcc tgatacgcgc
    24121 ctccagtatg gatccccaca atgcggtgac gagttgtttg cgctttgcgg gaggtgatgg
    24181 agatcttctg ccccagcagt ttgttcaaca atgtggattt gccaacgttc ggacgtccga
    24241 cgatggcaat aaatccgcag taacttttat cgatgctcat tccagctcca gttttttcaa
    24301 cgcctgttcg gcggcagcct gctcagcctt acgacggctt gaacctgtgc caaccaccgg
    24361 ttcactcagg ccgctgacct ggcagtggat agtaaattcc tgatcgtgcg cttcgccacg
    24421 tacctggact accagataag tcggcagcgg cagatggcga ccctgcaaat attcttgcaa
    24481 gcgcgttttc ggatcttttt gtttatcgcc tgggctaatt tcgtccaaac gagtttgata
    24541 ccagttgagg attaatttct cgacggtttg aatatcactg tcgaggaata cgccaccaat
    24601 taatgcttcg acggtgtcgg cgagaattga ctcacgacga aatccaccgc ttttaagttc
    24661 acctggccct aaacgtaagc actcgcctaa ctcaaattcg cgcgccagtt ccgccagcgt
    24721 attgccacgg accagcgtgg cgcgcatccg gctcatatcg ccttcatcca cacgagggaa
    24781 acggtgataa agcgcattgg cgataacgta gctcagaata gagtcgccta aaaattctaa
    24841 acgctcgtta tgtttactgc tggcactacg atgagttaat gcctgctgca acagttcctg
    24901 atgattaaaa gtgtagccca gcttccgttg aagccgatta attacgatgg ggttcatgcg
    24961 ataccaataa ataaatgcgt caacaattca gcacacgaaa cagacctgat atacatggct
    25021 ctgctaactg cttcgctgca gtttcagttt accggtatat ggggaccaac gctgtttcgt
    25081 gtgccgtggc aacctggagg tgccaacctt aaacttcggg ggaatattct atacacaacg
    25141 acgggggatg tcgttagcca cgggagattt atctcataaa taattcacgt tgtcgccata
    25201 acggcgacaa cgtgaacgaa gatgagctat taatggatgc cgccaatgcg acttaagcgc
    25261 acaccagtcg gccattcgcc ttcttgctta tcgaagctca tccagatagc cgttgcccga
    25321 ccgaccagat tcgcttcagg cacaaacccc caataacggc tgtccgcgct gttgtcgcgg
    25381 ttgtcgccca tcatgaagta ttgtcccgga ggaacaatcc aggttgccag ttgttgccct
    25441 ggctgctggt aatacatccc cacctgatcc tgcgcaatcg gcactgtcag aattcggtgc
    25501 gtcacatcac ccagtgtctc tttacgctcg gaaagacgaa ttccattttc tttggtttcg
    25561 tttttcggca cttcaaagaa tccgctggtc gcttccccac cattacggcg tgagaaggtc
    25621 tgaacgaaat cgctcggttc cacgtttgag taggtgaccg gcagcgcgtt ttcacacgcc
    25681 tggccggaac tgcatcccgg ttgaatcgtc agctctttcg agaccggatc ataagtaact
    25741 ttatcgcccg gtaaacccac cgcgcgcttg atgtaatcaa gctttggatc ttccggatat
    25801 ttaaagacca cgatatctcc gcgtttcgga tgaccggttt cgatcagcgt tttctggtag
    25861 ataggatctt taatgccata agcaaacttt tctaccagaa taaaatcacc gattaacaga
    25921 gtcggcatca tcgaacctga cgggatctgg aacggttcat aaataaacga acgcacaatc
    25981 aatacgatag ccagtaccgg aaaaacagaa gcaccggttt ccagccagcc aggcttcggc
    26041 gcaacctttt tcaacgttgc tttatccagt gagtcaccgg cagccgcctg cgccgctgcc
    26101 tgacgttccc gccgtttagg tgcgaaaaag aatttatcca cgcaccataa aatgcccgtc
    26161 accagtgtgg caatcaccag aatcagggca aacatattcg ccatgccaac tcctaagggt
    26221 tatttgttgt ctttgccgac gtgcagaatg gcgaggaacg cttcctgcgg cagctcgacg
    26281 ttaccgatct gcttcatgcg tttcttacct tctttctgct tctgcagcag ctttttctta
    26341 cggctgatat cgccgccata acatttagcc agtacgtttt tacgcagctg tttcacggtg
    26401 gatcgcgcaa taatgtgcgt accaatcgct gcctgaatgg cgatatcaaa ctgctggcgt
    26461 gggatcagat ctttcatctt ctccaccaac tcgcgaccgc ggttttgcga attatcacgg
    26521 tgggtgatca gcgccaacgc atcaacacgt tcaccgttga ttaatacgtc tacacgtacc
    26581 atgtcggacg cctggaagcg tttgaagttg taatccagag acgcataacc acgcgaggta
    26641 gatttcaggc gatcgaagaa gtcgagcacc acttccgcca tcgggatctc gtacgtcagc
    26701 gccacctgat taccgtggta aaccatattg gtctgcacgc cgcgtttttc tacgcacagc
    26761 gtaataacgt tgccgagata tgcctgcggc agcagcatgt gacattcggc aatcggctcg
    26821 cgcagttcgt agatgttatt taccgctggc agcttggatg gactgtcgac gtaaataact
    26881 tctcttgacg tggtttcaac ttcatacact acggtcggcg cagtggtgat cagatccaga
    26941 tcgtattcac gttccagacg ttcctggatg atctccatgt gcagcaggcc gaggaagccg
    27001 cagcggaaac caaagcccag cgcgctggag ctttccggct catagaacag tgaagcatcg
    27061 ttcaggctga gtttacccag cgcgtcacgg aaggcttcat agtcgtcgga acttaccggg
    27121 aacagaccgg cgtatacctg cggtttgact ttcttaaagc caggcagcgc cttttctgcc
    27181 ggattacgcg ccagcgttaa ggtatcgccg actggagcgc cgtggatatc tttaatcgca
    27241 cataccagcc agcctacttc gccacatttc agttcagtgc ggtcaacctg tttcggcgtg
    27301 aagatgccca gacggtcggc gttataggtc tgcccggtac tcatgacttt cactttgtcg
    27361 cccttacgca gggtgccgtt tttaatacgg ataagtgaaa caacgcccag gtagttgtcg
    27421 aaccatgagt cgataattag tgcctgcaac gggccttccg gatcgccttc cggcggcgga
    27481 atgtcgcgca ccagacgttc gagaacgtcc tgcacgccaa cgccggtttt cgctgaacag
    27541 cgcaccgcgt cggtggcgtc gatgccgacg atatcttcaa tttcttccgc cacgcgttca
    27601 ggatcggctg ccggcaggtc aatcttgttc agtaccggca ccacttcgag atccatttcc
    27661 atggcggtgt agcagtttgc cagggtttgc gcttctacgc cctgcccggc gtcgaccacc
    27721 agcaatgcac cttcacaggc agccagcgaa cgggaaactt cataggagaa gtcaacgtgg
    27781 cccggggtgt cgataaagtt cagctgatag gtttcgccgt cagacgcttt gtagtccagc
    27841 gtcacgcttt gcgctttgat ggttatgcca cgctcacgct caagatccat ggaatcgaga
    27901 acctgcgcct ccatttcacg gtcagacagg ccaccgcaga tctggataat acggtcagac
    27961 agcgtcgatt taccgtggtc aatgtgagct atgatcgaaa agttacgtat attcttcata
    28021 aagtatgatt attgtgcctt aatgcccggt taaccaggct tttagaagtc gctgttctga
    28081 gcttaacgtc tgtattaata gaaacgccgc attctacact acaacattga ggcgaggaaa
    28141 tgttcatacc gtatggattg tggtatctgg aaacgtcctc gcatttgtta tgcaaaatgc
    28201 aacaaagcca gtgaaatcac tggctcgcgt cttccgaaga tgtttcaaat cgcacaaggc
    28261 caggcggcaa tgccacgctt aagatgatcg gttgccattc tgcccgggca gcaaacttac
    28321 gcgagtagcc gcgcgcaatc aggaacccgc caataccgcc gaggaccgca ccacataatg
    28381 ctgcgacgtc agaagcaaag agtagctgaa atagcgaagc gatgaggaat aatcccacca
    28441 gcggcgacat ataaaccagt aatgcggagc taagcaggct gccttcggcg atccccaatt
    28501 ccactttttg ccccggcact aacggctcat cacagggtac gacaatggta tgcgtggttt
    28561 gcgggccaag tttatttaac acgcggctac cgcaaccggc gcgtgaagcg cagctgctgc
    28621 atgaagcttt aacatcacaa ctgaccagcg cctgcccgtt ttgccaggag acgacggtag
    28681 cccactcttt gatcattgcg ctgccccgaa cttaatattc tcggcaatgc gtttcgccgt
    28741 ttgcggcggc agttcaccga caatggtgat ttcggcgtta tcacgtacgc ttgtactgac
    28801 ggttctgcgt ccggtgcgca acatctgatc ggtgctcgat ggcgtagcgc ggttaacgtt
    28861 taccgagaag ctgaataatc cgtcggaata gagacgtgat tcgataggca tgttgtccat
    28921 cgtcggtagc ggacgtcgac tactggaaac ttcgctaaaa ccctgtggca accaggttgg
    28981 cgtccagctg aatttagctt tttcacctac aggaacagaa agcaacggcg gcaaatttgc
    29041 cttcgccagc gtctgcatac tgctgctgat atcctgattg acgttaaaag caatcacgcg
    29101 aaattgttcc agcgtttcac catcgcgatc aaggagatca acccgcatcg gtaatttcga
    29161 ttcggtgtcc atccacacga tgtagctgta gcgtgtacca tctcgggcaa ccacgcgaat
    29221 gacttcgcaa agacgatcag caatacgcgt gcgcccgacg gagataaagt cgtagtaagg
    29281 agaaaggtgt ttgaaatcgg tatagataag cgatggcaga gaatcaacaa tgtaatcgcc
    29341 attaagcgtg aacggttcaa gtcccggttc aaaatagctg atttcattgc cgcgctgtac
    29401 cacttcccgg cgcgggccat ccatttgcaa caattgtgca agaggacggt tatcgaggcg
    29461 tgcatgtcga taacgcagag actcaacacc ctgtttattg atgctgatga atgacagctc
    29521 gtaattcagt gactgactgg ccaggttcat ctgctgtaat aacgccccgg acgcgggagt
    29581 ggccgaggcg ttagcagaga ataacaggct acctgtcact aatgacatgg caaaccaaag
    29641 ttgcttcatt actgcgattg cgttcctaaa gtttgaattc ctggcacctg tacagcggct
    29701 tgctgggttt gcgcctgctc aaactgaagc tgttcagagt ggagtcggcg ttgcagttcg
    29761 taatcctgca acattgcatt aatgcgacga cgctgctcct gtacctgctg ctgttgacca
    29821 ttgtttgcgg tcgcttcaga aggtactccc aggcttaccg ggctggcttt acccatcatc
    29881 ggcagtgtat taaataccgg cgtttcgggc tgctgggacg tttcagattg tccattatag
    29941 tgctggacgc caacgataac tgcaagcgat acgcatgctg ctacgcccat ttgggtaagc
    30001 tgtgccgccc acggacgtac tttctgccag aatggcattt tctgccattg atgcggcgca
    30061 ggctgggctt ccgggatcaa tgtcgccggt tgacgtactg gctcttcttc aatggcggcc
    30121 atcacgcgtg aagagatatc gaaatggagc acctcgggag tatcaccccg cattgagtca
    30181 cggattaagt gatagctttc ccaggttttc tgcatttctg ggttatgagc cagttcgtta
    30241 agcagctcac tatccagcgt ttcgccatcc attaaagcgg aaagttgttc tttctgcatg
    30301 cctaataccc ttatccagta tcccgctatc gtcaacgcct gataagcggt tgaactttgt
    30361 tatcaatagc ttccctcgct cggaagatac gtgaacgcac cgtacctacc ggacaatcca
    30421 tgatagcggc tatctcttca tagctcaggc catccagctc ccgcaaggtt attgccatgc
    30481 gcaaatcttc cgggagggac tcaatagttc ggaaaactat ctgtctcagt tcttctgaca
    30541 acattaagtt ctcagggttc gaaatttctt tcaacgcgcc gccactttcg aagttttcag
    30601 cttcaatggc atccacatca ctggaaggtg gacgacgccc ctgagcaacc aggtaatttt
    30661 tcgctgtatt tacagcaatc cgatacagcc atgtataaaa agcgctatct ccccggaacg
    30721 aatccagcgc acgataggct ttaataaaag cttcttgtac cacatcggga acatcacccg
    30781 acggcacata gcgggaaacc agactcgcca ctttatgctg atagcgcact accagtaggt
    30841 taaaggcttt ctgatctccc ttctggaccc gttcaaccag gacctggtcc gttaactgct
    30901 cgctcatccg aggtaaagtc tccccaaacc aaatttccac gcgctatcga aacgccactc
    30961 cattagctgc aatttgagca agcaaagggt tagagtgtct cgtttttgta aagttccgta
    31021 acgcatctgt ttttgtttgt catgctgtag acggatcatt atctatcatt ataagtctac
    31081 agaatctgaa catcgcatta tctgtgtaga aatgcccatt taactgcctg aagagtaacc
    31141 caacggcctt tttatttcac cacctaatcc tccaccagcc agtaacttct ctttttctcg
    31201 ccgccctgcg tcagcgtgtt tagccactgt aacaaatatt aaaatagcag gtgtttattc
    31261 gcacaacatg atgctatgct gaccaaacag tgtttagtaa attaaacaaa gaaaatgaat
    31321 actctcccag aacattcatg tgacgtgttg attatcggta gcggcgcagc cggactttca
    31381 ctggcgctac gcctggctga ccagcatcag gtcatcgttc taagtaaagg cccggtaacg
    31441 gaaggttcaa cattttatgc ccagggcggt attgccgccg tgtttgatga aactgacagc
    31501 attgactcgc atgtggaaga cacattgatt gccggggctg gtatttgcga tcgccatgca
    31561 gttgaatttg tcgccagcaa tgcacgatcc tgtgtgcaat ggttaattga ccaaggggtt
    31621 ttgtttgata cccacgttca accgaatggc gaagaaagct accatctgac ccgtgaaggt
    31681 ggacatagtc accgtcgtat tcttcatgcc gccgacgcca ccggtagaga agtacaatcc
    31741 acgctggtga gcaaggcgca gaaccatccg aatattcgtg tgctggagcg cagcaacgca
    31801 gttgatctga ttgtttctga caaaattggc ctcccgggca cgcgacgggt tgttggcgcg
    31861 tgggtatgga accgaaataa agaaacggtg gaaacctgcc acgcaaaagc agtggtactg
    31921 gcaaccggcg gtgcgtcgaa ggtttatcag tacaccacca atccggatat ttcttctggc
    31981 gatggcattg ctatggcgtg gcgcgcaggc tgtcgggttg ccaatctcga atttaatcag
    32041 ttccacccta ccgcactgta tcacccacag gcacgcaatt tcctgttaac ggaagcactg
    32101 cgcggcgaag gcgcttatct caagcgcccg gatggcacgc gttttatgcc cgattttgat
    32161 gagcgcggcg aactggcccc gcgcgatatt gtcgcccgcg ccattgacca tgaaatgaaa
    32221 cgcctcggcg cagattgtat gttcctcgac atcagccata agcccgccga ttttattcgc
    32281 cagcatttcc cgatgattta tgaaaaattg ctcgggctag ggattgatct cacacaagaa
    32341 ccggtaccga ttgtgcctgc tgcacattat acctgcggtg gtgtaatggt tgatgatcat
    32401 gggcgtacgg acgtcgaggg cttgtatgcc attggcgagg tgagttatac cggcttacac
    32461 ggcgctaacc gcatggcctc gaattcattg ctggagtgtc tggtctatgg ctggtcggcg
    32521 gcagaagata tcaccagacg tatgcctgat gcccacggcg tcagtacgtt accgccgtgg
    32581 gatgaaagcc gcgttgagaa ccctgacgaa cgggtagtaa ttcagcataa ctggcacgag
    32641 ctacggctgt ttatgtggga ttacgttggc attgtgcgca caacgaagcg cctggaacgc
    32701 gccctgcggc ggataaccat gctccaacaa gaaatagacg aatattacgc ccatttccgc
    32761 gtctcaaata atttgctgga gctgcgtaat ctggtacagg ttgccgagtt gattgttcgc
    32821 tgcgcgatga tgcgcaaaga aagccggggg ctgcatttta cgctggatta cccggaactg
    32881 ctcacgcatt ccggtccatc gatcctttcc cccggcaatc attacataaa cagataaaaa
    32941 gcctgggtca gcgccgtata cgcttcggaa tagttctggt ctggcccacg aatgactaag
    33001 cgatcgctaa agcattctcc cgcctgcggg gagaatgcca gcagcacccg atgcggcagt
    33061 cgcgcttcgt tttccgccac atccgtccgc aaacgtaaat gccagcccat gcttaatgcc
    33121 agttcagtaa aaccattacc aatctgctct ggcagcacca cgcagaaaaa tccctcttcg
    33181 gtaatgcact ccgccgcaca ggtcagcagc gatgggtgat caagcgtagt ggtatagcga
    33241 gcctgttccc gttgaggtgt cgcgcactct actccctgct gatagtaagg tgggttactg
    33301 atgattaaat cgaagcgtac tgtctgctgt gtgatccact gctgaatatc cgccgtatgg
    33361 acgttaatcc gctctgccca cggggactgg ttgatatttt cctgcgcctg cgcggcagct
    33421 tcactttcca gctcaacggc atcaattatc acgctgtcac tggttcgctg cgccagcatc
    33481 agtgccagca acccgctacc cgcgccgata tcaaggcaac gtttcacccc agccaccggt
    33541 gcccatgcgc ccagcaaaat gccatccgtt cccactttca tcgcacagcg atcgtgagca
    33601 acaaaaaact gtttaaaagt aaatccatta cgacgaagca cggatgtaga ctgtgacatg
    33661 aaaataaaac cttgcaggaa aaacggcgat agcaccgggt gagaacaata cccgagaagc
    33721 gatatccata caaacagatg aagattgcag ccgtaacgtc tataatcagc gccccacaca
    33781 gaggtagaac atgactgtaa cgactttttc cgaacttgaa ctcgacgaaa gcctgctgga
    33841 agccctccag gataaaggtt tcactcgccc gaccgccatt caggctgccg ccattccgcc
    33901 tgcgctcgat ggccgtgatg tactcggttc tgcgccgaca ggcaccggta aaacggcggc
    33961 gtatctgctg ccagcgttgc agcacctgct cgatttcccg cgtaagaaat ccggtccgcc
    34021 gcgtattttg atcctcaccc caactcgcga gctggcgatg caggtggccg atcatgcccg
    34081 cgaactggcg aaacatacgc atctggatat cgccaccatc accggcggcg tagcctatat
    34141 gaaccacgcg gaagtgttca gcgaaaatca ggacatcgtg gtcgccacga ccggacgtct
    34201 gctgcaatac ataaaagaag agaacttcga ttgccgcgcg gttgaaacgc tgatcctcga
    34261 cgaagcagac cgtatgctgg atatgggctt cgctcaggat atcgaacata ttgctggcga
    34321 aacgcgctgg cgtaaacaga ccctgctctt ttcggcaacg ctggaaggcg atgcgattca
    34381 ggactttgcc gagcgtctgc tggaagatcc ggtggaggtt tctgccaatc cgtccacccg
    34441 tgagcgcaaa aaaattcatc agtggtatta ccgcgccgat gacctggaac ataaaaccgc
    34501 attgctggtg catctgttaa aacagccgga agcgacccgc tcaattgtgt ttgtgcgtaa
    34561 gcgtgaacgt gtgcatgagc tggcaaactg gctgcgcgaa gcgggcatca acaactgcta
    34621 tctcgaaggt gagatggtac aaggcaagcg taacgaagcg atcaagcgtt tgaccgaagg
    34681 tcgcgtaaac gtactggttg caaccgatgt tgccgcgcgc ggtatcgaca ttcctgacgt
    34741 cagccacgtc tttaacttcg atatgccgcg cagtggcgat acttatttgc accgtatcgg
    34801 acgtaccgcg cgcgccggtc gtaaaggcac cgcaatttcg ctggtggaag cccatgacca
    34861 tttgctgctg ggcaaggttg gccgttacat tgaagagccg attaaagctc gcgttattga
    34921 tgagttacgc ccgaaaacgc gtgcgccaag cgagaagcag accggcaagc catcgaagaa
    34981 agtactggca aaacgcgccg agaagaaaaa agctaaagag aaagagaaac cgcgtgtgaa
    35041 aaaacgccat cgcgacacca aaaatattgg taagcgccgt aaaccaagcg gaacgggcgt
    35101 gccaccgcaa acgacagaag agtaatctca atgccgggtt taagcctggt attaaaagtg
    35161 ctg
//
LOCUS       DAZFVO020000032        35109 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFVO020000032 DAZFVO020000000
VERSION     DAZFVO020000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.032"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            29..1236
                     /locus_tag="ACHKNB_RS07430"
                     /old_locus_tag="pgaptmp_001486"
     CDS             join(29..350,350..1236)
                     /locus_tag="ACHKNB_RS07430"
                     /old_locus_tag="pgaptmp_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4195029.1"
                     /translation="MTGILLGQELRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQIKELQRLLGKKTMEVEILKEAV
                     EYGQSPKMDSACALVANGQGITLVSRILGVSRAQLAQRMKTSGNKPNKRRRRCDEAAD
                     AEVLARILDIISDMPTYGYRRVWAILRRQSRNKGVPFVNAKRVYRIMSENNLLLLHDK
                     PSRPQREHKGRISVKESDQRWCSDGFEFGCDDGAKLRVTFALDCCDREAIDWAASTGG
                     YDKATVQDVMLGAIEKRFGDKGAERPIQWLTDNGSAYTAHETRQFARELNLEPCTTAV
                     SSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNETHPHSALGYLSPR
                     EYRRQRVTST"
     gene            complement(1570..2754)
                     /locus_tag="ACHKNB_RS07435"
                     /old_locus_tag="pgaptmp_001487"
     CDS             complement(1570..2754)
                     /locus_tag="ACHKNB_RS07435"
                     /old_locus_tag="pgaptmp_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4195030.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3054..3148)
                     /locus_tag="ACHKNB_RS07440"
                     /old_locus_tag="pgaptmp_001488"
     tRNA            complement(3054..3148)
                     /locus_tag="ACHKNB_RS07440"
                     /old_locus_tag="pgaptmp_001488"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3112..3114),aa:Sec,seq:tca)
     gene            3441..4823
                     /gene="yicJ"
                     /locus_tag="ACHKNB_RS07445"
                     /old_locus_tag="pgaptmp_001489"
     CDS             3441..4823
                     /gene="yicJ"
                     /locus_tag="ACHKNB_RS07445"
                     /old_locus_tag="pgaptmp_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HGU4195031.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            4833..7151
                     /gene="yicI"
                     /locus_tag="ACHKNB_RS07450"
                     /old_locus_tag="pgaptmp_001490"
     CDS             4833..7151
                     /gene="yicI"
                     /locus_tag="ACHKNB_RS07450"
                     /old_locus_tag="pgaptmp_001490"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HGU4195032.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(7204..8913)
                     /gene="yicH"
                     /locus_tag="ACHKNB_RS07455"
                     /old_locus_tag="pgaptmp_001491"
     CDS             complement(7204..8913)
                     /gene="yicH"
                     /locus_tag="ACHKNB_RS07455"
                     /old_locus_tag="pgaptmp_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4195033.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(9034..10425)
                     /gene="xanP"
                     /locus_tag="ACHKNB_RS07460"
                     /old_locus_tag="pgaptmp_001492"
     CDS             complement(9034..10425)
                     /gene="xanP"
                     /locus_tag="ACHKNB_RS07460"
                     /old_locus_tag="pgaptmp_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HGU4195034.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            10705..11910
                     /gene="gltS"
                     /locus_tag="ACHKNB_RS07465"
                     /old_locus_tag="pgaptmp_001493"
     CDS             10705..11910
                     /gene="gltS"
                     /locus_tag="ACHKNB_RS07465"
                     /old_locus_tag="pgaptmp_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HGU4195035.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            11913..12779
                     /locus_tag="ACHKNB_RS07470"
                     /old_locus_tag="pgaptmp_001494"
     CDS             11913..12779
                     /locus_tag="ACHKNB_RS07470"
                     /old_locus_tag="pgaptmp_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195036.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(12764..14845)
                     /gene="recG"
                     /locus_tag="ACHKNB_RS07475"
                     /old_locus_tag="pgaptmp_001495"
     CDS             complement(12764..14845)
                     /gene="recG"
                     /locus_tag="ACHKNB_RS07475"
                     /old_locus_tag="pgaptmp_001495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGU4195037.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(14851..15540)
                     /gene="trmH"
                     /locus_tag="ACHKNB_RS07480"
                     /old_locus_tag="pgaptmp_001496"
     CDS             complement(14851..15540)
                     /gene="trmH"
                     /locus_tag="ACHKNB_RS07480"
                     /old_locus_tag="pgaptmp_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HGU4195038.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(15547..17655)
                     /gene="spoT"
                     /locus_tag="ACHKNB_RS07485"
                     /old_locus_tag="pgaptmp_001497"
     CDS             complement(15547..17655)
                     /gene="spoT"
                     /locus_tag="ACHKNB_RS07485"
                     /old_locus_tag="pgaptmp_001497"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HGU4195039.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(17674..17949)
                     /gene="rpoZ"
                     /locus_tag="ACHKNB_RS07490"
                     /old_locus_tag="pgaptmp_001498"
     CDS             complement(17674..17949)
                     /gene="rpoZ"
                     /locus_tag="ACHKNB_RS07490"
                     /old_locus_tag="pgaptmp_001498"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGU4195040.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(18004..18627)
                     /gene="gmk"
                     /locus_tag="ACHKNB_RS07495"
                     /old_locus_tag="pgaptmp_001499"
     CDS             complement(18004..18627)
                     /gene="gmk"
                     /locus_tag="ACHKNB_RS07495"
                     /old_locus_tag="pgaptmp_001499"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGU4195041.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            18885..20567
                     /gene="ligB"
                     /locus_tag="ACHKNB_RS07500"
                     /old_locus_tag="pgaptmp_001500"
     CDS             18885..20567
                     /gene="ligB"
                     /locus_tag="ACHKNB_RS07500"
                     /old_locus_tag="pgaptmp_001500"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HGU4195042.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEDISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNARDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(20564..21181)
                     /gene="yicG"
                     /locus_tag="ACHKNB_RS07505"
                     /old_locus_tag="pgaptmp_001501"
     CDS             complement(20564..21181)
                     /gene="yicG"
                     /locus_tag="ACHKNB_RS07505"
                     /old_locus_tag="pgaptmp_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HGU4195043.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(21473..22297)
                     /gene="dinD"
                     /locus_tag="ACHKNB_RS07510"
                     /old_locus_tag="pgaptmp_001502"
     CDS             complement(21473..22297)
                     /gene="dinD"
                     /locus_tag="ACHKNB_RS07510"
                     /old_locus_tag="pgaptmp_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HGU4195044.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(22518..23381)
                     /gene="yicC"
                     /locus_tag="ACHKNB_RS07515"
                     /old_locus_tag="pgaptmp_001503"
     CDS             complement(22518..23381)
                     /gene="yicC"
                     /locus_tag="ACHKNB_RS07515"
                     /old_locus_tag="pgaptmp_001503"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HGU4195045.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            23508..24224
                     /gene="rph"
                     /locus_tag="ACHKNB_RS07520"
                     /old_locus_tag="pgaptmp_001504"
     CDS             23508..24224
                     /gene="rph"
                     /locus_tag="ACHKNB_RS07520"
                     /old_locus_tag="pgaptmp_001504"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HGU4195046.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            24290..24931
                     /gene="pyrE"
                     /locus_tag="ACHKNB_RS07525"
                     /old_locus_tag="pgaptmp_001505"
     CDS             24290..24931
                     /gene="pyrE"
                     /locus_tag="ACHKNB_RS07525"
                     /old_locus_tag="pgaptmp_001505"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGU4195047.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(24968..25564)
                     /gene="slmA"
                     /locus_tag="ACHKNB_RS07530"
                     /old_locus_tag="pgaptmp_001506"
     CDS             complement(24968..25564)
                     /gene="slmA"
                     /locus_tag="ACHKNB_RS07530"
                     /old_locus_tag="pgaptmp_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HGU4195048.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(25671..26129)
                     /gene="dut"
                     /locus_tag="ACHKNB_RS07535"
                     /old_locus_tag="pgaptmp_001507"
     CDS             complement(25671..26129)
                     /gene="dut"
                     /locus_tag="ACHKNB_RS07535"
                     /old_locus_tag="pgaptmp_001507"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGU4195049.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(26107..27327)
                     /gene="coaBC"
                     /locus_tag="ACHKNB_RS07540"
                     /old_locus_tag="pgaptmp_001508"
     CDS             complement(26107..27327)
                     /gene="coaBC"
                     /locus_tag="ACHKNB_RS07540"
                     /old_locus_tag="pgaptmp_001508"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HGU4195050.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            27499..28167
                     /gene="radC"
                     /locus_tag="ACHKNB_RS07545"
                     /old_locus_tag="pgaptmp_001509"
     CDS             27499..28167
                     /gene="radC"
                     /locus_tag="ACHKNB_RS07545"
                     /old_locus_tag="pgaptmp_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGU4195051.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            28384..28620
                     /gene="rpmB"
                     /locus_tag="ACHKNB_RS07550"
                     /old_locus_tag="pgaptmp_001510"
     CDS             28384..28620
                     /gene="rpmB"
                     /locus_tag="ACHKNB_RS07550"
                     /old_locus_tag="pgaptmp_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGU4195052.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            28641..28808
                     /gene="rpmG"
                     /locus_tag="ACHKNB_RS07555"
                     /old_locus_tag="pgaptmp_001511"
     CDS             28641..28808
                     /gene="rpmG"
                     /locus_tag="ACHKNB_RS07555"
                     /old_locus_tag="pgaptmp_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGU4195053.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            28906..29715
                     /gene="mutM"
                     /locus_tag="ACHKNB_RS07560"
                     /old_locus_tag="pgaptmp_001512"
     CDS             28906..29715
                     /gene="mutM"
                     /locus_tag="ACHKNB_RS07560"
                     /old_locus_tag="pgaptmp_001512"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HGU4195054.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(29754..30233)
                     /gene="coaD"
                     /locus_tag="ACHKNB_RS07565"
                     /old_locus_tag="pgaptmp_001513"
     CDS             complement(29754..30233)
                     /gene="coaD"
                     /locus_tag="ACHKNB_RS07565"
                     /old_locus_tag="pgaptmp_001513"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGU4195055.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(30241..31518)
                     /gene="waaA"
                     /locus_tag="ACHKNB_RS07570"
                     /old_locus_tag="pgaptmp_001514"
     CDS             complement(30241..31518)
                     /gene="waaA"
                     /locus_tag="ACHKNB_RS07570"
                     /old_locus_tag="pgaptmp_001514"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HGU4195056.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31931..32989
                     /gene="rfaQ"
                     /locus_tag="ACHKNB_RS07575"
                     /old_locus_tag="pgaptmp_001515"
     CDS             31931..32989
                     /gene="rfaQ"
                     /locus_tag="ACHKNB_RS07575"
                     /old_locus_tag="pgaptmp_001515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HGU4195057.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            32986..34110
                     /gene="waaG"
                     /locus_tag="ACHKNB_RS07580"
                     /old_locus_tag="pgaptmp_001516"
     CDS             32986..34110
                     /gene="waaG"
                     /locus_tag="ACHKNB_RS07580"
                     /old_locus_tag="pgaptmp_001516"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGU4195058.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            34103..34900
                     /gene="rfaP"
                     /locus_tag="ACHKNB_RS07585"
                     /old_locus_tag="pgaptmp_001517"
     CDS             34103..34900
                     /gene="rfaP"
                     /locus_tag="ACHKNB_RS07585"
                     /old_locus_tag="pgaptmp_001517"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HGU4195059.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         8408 a         8610 c         9321 g         8770 t
ORIGIN      
        1 tatgtctaac actagtgcta attttgagat gactggaatt ttgctggggc aggagcttcg
       61 taaacgtaaa actcctcagg aaaaaatcgc cattattcag cagacaatgg aacccggtat
      121 gacggtgtcc catgtggcgc gtttgcatgg tattcagccc agcctgttgt ttaagtggaa
      181 aaaacaatat ctggaaggaa gtctcactgc tgttgctgcc ggagaggatg tcgttcctgc
      241 ctcagaactg gctgccgcta taaaacaaat aaaagagtta cagcgcttgc tgggtaagaa
      301 aaccatggaa gttgagatcc tcaaagaagc ggtggagtat ggtcagtcgc gaaaatggat
      361 agcgcatgcg cccttgttgc caacggacaa ggaataaccc tggtaagccg tattctgggc
      421 gtgtcgcgtg cgcaactggc acaacggatg aaaacatctg gtaataagcc gaataaacgt
      481 cgtcgcagat gtgatgaggc tgcggatgcg gaagtgttag cacgtatcct tgatatcatc
      541 agcgatatgc cgacatatgg ctatcgtcgt gtctgggcca ttctcagacg acagtcccgt
      601 aacaaaggag tgccgttcgt gaatgcaaag cgcgtttatc ggataatgag cgaaaataac
      661 ctgttgttgt tacatgacaa accttcacgg ccgcagcggg aacataaagg ccgaatatcg
      721 gtgaaggaaa gcgaccagcg ctggtgttcc gatggctttg agttcggttg tgatgatggc
      781 gcaaaactgc gtgtcacgtt cgcactggac tgttgcgatc gtgaagccat agactgggcc
      841 gcaagcacgg gaggttatga caaagcaacg gtgcaggatg tcatgttagg agcaatagaa
      901 aaacgctttg gcgataaagg agcggaacgg ccaatccagt ggctgactga taacggttcg
      961 gcttatacag ctcatgaaac acggcagttc gccagagagt tgaatctgga gccctgcacg
     1021 acggcagtca gtagccctca gagcaatgga atggcagaaa ggttcgtgaa aacgatgaaa
     1081 gaagactaca tcgcgttcat gccgaaaccg aatgtaagaa cggcattgca taaccttgca
     1141 gtggcgatcg agcattacaa tgaaacccat ccgcatagtg cgttaggtta tctctcacca
     1201 agggaatatc gacgtcagcg ggtaacgtca acttaagata caacgactgt ctgaatatcg
     1261 tgggtcaagg acactttcct tgagaacaat aattttatct ttatgtgaat ctcatttttt
     1321 ataatgatgt cagatgattg aaatgctaaa ttatatctga atcaactttt cctttgctaa
     1381 aggtatcgaa ataactgcaa ggctgtattt tgtaattgcc aatttaaaaa tgtcaatttg
     1441 cttttaatta tgtttttgta ataagtttga agacgcaaaa aataatataa taacaaaaat
     1501 cctccagcct tgtaggccag aggataaaat acttaattga ggaaaacatc agggaaaagt
     1561 atttctgact taactttccc ggctggagag tgaatcaatg taatcagcgt accattgcat
     1621 catttcacgg cggccatcga gatacagggc atgattatag gtacccctga tggaattttt
     1681 atccacatga gctaactgca tttcaatcca ggcggaatta aagccttgtt catgcagaat
     1741 tgtactcatg gtgtgtctga aaccgtgccc tgtgagtctt cccttatatc caagtaattc
     1801 aattacttgg ttaatgcttt ccttactaat tggtttgcgg ggatcatttc taccgataaa
     1861 gactagggga tgatgttttg agattggttc aagctgctta aatagcataa tgacctgctc
     1921 agagagcggc acaatatgag gtcgcttcat cttcattact tctgccggga tctcccacaa
     1981 ttttgtctca aaatcgatat cttcccagcg tgcaaaacgc agttcttgtg ttcgtacgcc
     2041 ggttaacata atgatctgag tagctgtctt agtgattata cttccggtat aacccgccag
     2101 atcgttgagg aagtggggaa gttcattagc agtaagaaag ggaaaatgta cttttttcgg
     2161 tgtagctaaa gcactggcaa gatcgggggc tgggttatag tctgcccggc cagtaacaat
     2221 tgcatagcgg aacacctcac cacagcgctg gcggaccttg cgcattttct ccagagctcc
     2281 acgtttttcc attttacgca gtgcttccag taactccatt ggtttgattt cggcaatagg
     2341 gcgcttgcca atgtagggaa aaatgtcttt ttcaaaggta tcaatgattt catcacgata
     2401 gcgtaatgac caccgatcgg cttttgactg gtgccattcg cgagcaatag cctcgaacgt
     2461 atttttcaga ctcatttgct gcgcgatttt ttcttccttt tttgcctccc ctggatcgcc
     2521 tccagcagcg aggagtttcc tggcttcact gcgtttctcg cgagcatcgg ctagcgttac
     2581 atcggggtat acgccaatag aaagcatctt ttctttacca gcaaagcggt acttcatgcg
     2641 ccagtattta gaaccattag tgttaatcag tagatacata ccgccgccat cagcaagctt
     2701 atagggtttg tctttgggtt ttgcggtttt cactttgata tcggttaatg ccatgacatc
     2761 ctccggcttt tgatgaattc atgttcctga gggggatttc tgccagtaac taacctgata
     2821 cccccaatac accccttgta taacaaagat gttgttaaaa gccgttgtta tatatcacat
     2881 ttttcctatt atgggggtgc tggtatttca ataggaaata acacccccta aagtaccccc
     2941 aaaagaaact tgatacgggg tgaagtaggt tgacctctgg atacaaataa atactgatat
     3001 acaagggatt agaagagatt tattgacatt gggagagttc agaagaggta aactggcgga
     3061 agatcacagg agtcgaacct gcccgggacc gctggcggcc ccaactggat ttgaagtcca
     3121 gccgcctcac cggagacgac gatcttccgc gcctcgattg ctacatggag gcggggcgca
     3181 ttatagctac ttccttgagt ttctacatcc cccagatcag ttttttccgc ctaatacacg
     3241 cagcgtatta tcacgaaagc attatagttc atataaaacc catattttac aatgatatat
     3301 gagcggaatt aacgctacca cgatcacata aaacaagaat cataaattaa taaccagata
     3361 tcggaatatt cgctctcaca gggatggttt acaaaatgcg ttatcggtct cagcacccct
     3421 atagcatcaa ggaaaagcag atgaagagtg aagtgttgtc cgttaaagag aaaattggtt
     3481 atggcatggg agacgccgcc agccacatta ttttcgataa cgtaatgtta tatatgatgt
     3541 tcttttatac cgatattttt ggcattcctg ccggttttgt cggaaccatg tttttggtcg
     3601 ctcgtgcact ggatgcgatt tccgatcctt gcatggggtt gttggccgat cgaacgcgct
     3661 ctcgctgggg taaatttcgt ccgtgggtac tgtttggcgc actgccattc gggatcgtct
     3721 gtgtactggc ctatagcacg ccagatctca gtatgaacgg caaaatgatc tatgcagcaa
     3781 ttacttacac cctacttacc ttactttata ccgtcgtcaa tatcccttac tgcgcattgg
     3841 gtggtgtaat caccaatgac ccgactcagc gtatctcgct gcaatcctgg cgttttgtgc
     3901 tggcgaccgc gggaggcatg ctttctactg ttctgatgat gccactggtt aatttaattg
     3961 gcggtgataa taaaccactc ggtttccagg gcggtatcgc ggtcctttcc gtggtggcat
     4021 tcatgatgct ggcattttgt ttcttcacca ctaaagaacg cgttgaagca ccacctacaa
     4081 caacgtctat gcgggaagat ttacgtgata tctggcaaaa cgaccagtgg cggattgtcg
     4141 gtttactaac cattttcaat atcctggcgg tgtgcgtacg cggtggggcg atgatgtatt
     4201 acgtcacatg gattttgggc acgccggaag tgtttgtcgc ttttctcacc acttattgcg
     4261 tgggtaacct gattggttcc gcactggcaa aacctctgac cgactggaaa tgtaaagtca
     4321 ctatcttctg gtggacgaac gccctgctgg cagtgattag cctcgcgatg ttctttgttc
     4381 ccatgcaggc cagcatcact atgtttgtct tcatcttcgt gattggtgtg ttgcatcaac
     4441 tggtgacacc tatccagtgg gtaatgatgt ccgataccgt cgactacggc gagtggtgca
     4501 atggtaaacg cctgaccggg atcagttttg ctggcacgct gtttgtgctc aaactggggt
     4561 tggccttcgg cggcgctctt atcggctgga tgctggctta tggcggatat gatgcggcag
     4621 aaaaagcgca gaacagcgcc acgattagca tcattattgc gctattcacg attgttccgg
     4681 cgatctgtta tttgctgagc gcgattatcg ctaaacgcta ctactcactc acgacgcaca
     4741 atctgaaaac cgttatggaa cagctggctc agggtaaacg ccgttgccag caacaattca
     4801 cctctcaaga agtgcagaac taaggaacgg caatgaaaat tagcgatgga aactggttga
     4861 ttcaacctgg cctcaatttg attcacccgc ttcaggtgtt cgaggttgaa cagcaggata
     4921 atgaaatggt ggtctatgct gccccccgtg atgtgcgtga acgtacctgg cagcttgata
     4981 cgcctttatt tacgttgcgc tttttctccc cacaggaagg tattgtcggt gtgcggattg
     5041 agcattttca gggggcgctg aataacggtc ctcattatcc gctcaatatt ttgcaggacg
     5101 tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt taaaagtggc aacttaagcg
     5161 cgcgtgtcag caaaggtgag ttctggtcac tggattttct gcgcaacggc gaacgtatta
     5221 ccggtagtca ggtgaaaaat aatggctacg tgcaggacac gaataatcaa cgcaattata
     5281 tgtttgagcg gcttgatctt ggcgttggcg aaacagttta cggtctggga gagcgcttta
     5341 ctgccctggt gcgcaatggc cagacggtag agacctggaa ccgggacggc ggcacaagta
     5401 ctgaacaggc gtataaaaat atcccgttct acatgactaa ccgtggttat ggggtactgg
     5461 tcaatcatcc ccagtgtgtc tcttttgaag tgggatcgga gaaagtctcc aaagtgcagt
     5521 tcagcgttga gagtgaatat ctcgaatact ttgttatcga cggcccgacg ccgaaagcgg
     5581 tacttgatcg ttatacccgc tttactggtc gtccggcgct gccgcccgcg tggtccttcg
     5641 gcctgtggct aaccacttca tttaccacca actacgacga agcgacggta aacagcttta
     5701 tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt ccactttgac tgtttctgga
     5761 tgaaagcctt ccagtggtgc gattttgagt gggacccgct gactttccct gacccggaag
     5821 ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg cgtctggatt aacccctata
     5881 tcggtcaaaa atcccccgtc tttaaagagt tacaagagaa aggctattta ctcaaacgcc
     5941 ccgacggttc gctatggcag tgggataaat ggcagccagg tctggcgatt tatgacttta
     6001 ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa aggtctggtc gcgatgggcg
     6061 ttgattgctt taagaccgac tttggcgaac gtatcccaac tgatgttcag tggtttgacg
     6121 gttccgatcc gcagaaaatg cataaccatt atgcgtacat ctacaacgaa ctggtgtgga
     6181 acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt gtttgcccgc tcggcctccg
     6241 tcggtgcgca gaaattcccg gtacactggg gtggcgattg ttacgctaac tacgaatcaa
     6301 tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc aggttttggc ttctggagcc
     6361 acgatatcgg cggctttgaa aataccgctc cggcgcacgt ttacaaacgc tggtgcgcgt
     6421 ttggtttgct ctccagccat agccgtttac acggtagcaa atcttatcgt gtgccgtggg
     6481 cttacgatga tgagtcctgt gatgtggtgc gcttcttcac gcaactgaaa tgccgcatga
     6541 tgccgtatct gtatcgtgaa gctgcgcgtg cgaacgcgcg gggtacgccg atgatgcggg
     6601 ccatgatgat ggagttcccg gacgatccgg cttgtgatta ccttgaccgt caatacatgt
     6661 taggcgacaa cgtgatggtt gcgccggtgt tcactgaagc gggcgatgtg cagttctacc
     6721 tgccggaagg tcgctggaca cacctgtggc acaacgatga actcgacggt agtcgctggc
     6781 ataaacagca gcacggcttc ctgagtctgc ccgtttatgt gcgtgataac actctactgg
     6841 cgctgggcaa caacgatcaa cgtcccgatt acgtgtggca cgaaggcacg gcattccacc
     6901 tctttaatct gcaagacggg catgaagccg tctgtgaagt gcccgctgct gacggatcgg
     6961 tgatctttac tttaaaagca gcacgtactg gcaacacgat tactgtgact ggtgcgggcg
     7021 aggcgaagaa ctggacactg tgcctgcgca atgttgtgaa agtaaatggt ctgcaagacg
     7081 gttcgcaggc tgaaagtgag caggggctgg tggtgaagcc tcaagggaat gcgctgacaa
     7141 ttacgttgta atctattgat ggaatgccgg gtacgtctga gtcgtacccg gcgagattga
     7201 tgtttactgc accagctctg ccgacgtaac ttcaccgctg gaaacgatgc cagcattcat
     7261 ggtttgagtc acttgctgct ttgcggcgtt cacggcatgc aattgcccgc taaccgtagg
     7321 tttcagcggg gcattggcct gaatatctcc actggcggta agctgaatat tgccgtcgcc
     7381 agtcaacggt aattcaggcc atccccattg ttgcaaaata tttaccggca caccgcgtcc
     7441 attcaggctg atatgggtct gacgttgtgg cgtttgtgaa acactggcag tggcttccag
     7501 aatgcctttt tcagtaaatg cactcagttc gctgatattc accgtgctgc tgttggcggt
     7561 cagcgccagc gacgggcgac gaacatcaac acgattgaat gtcgcggcgg cggcattcag
     7621 attcgccgag ccactccaga cgccccattt atgatcggta accagcgtca ggttggcacc
     7681 gtaaccatcg agcgcggtga gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt
     7741 gcggctggcg ctaaatctct tcagttgcag gctgtttaac caaccgggtg tcgtttccat
     7801 ccacaactgt tgccagtttt tcggcaaggt atattccagc ccggcaattg ccgcatcatc
     7861 aaggatcaac gttttcccgt cacgcagcca gttccctgac gttctgacca tacccccttc
     7921 ccagcggctg gtgaactggc gcagcgctac gccctgcggg gaaaattccg tgtttataat
     7981 cgggtcaaat aaatgcagcg aaccataaat gaactcgcta gcgttcatcg acagtttgcc
     8041 ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc
     8101 ggtcaccgcc cagtccggac cttgcaaacg agcatcgatc acttccaggc gaccaatctg
     8161 caacgacggg acagagcgta atggcgcaaa gaagtcggtt agcgattttt cgctttgtag
     8221 acgtatatcc gccatgcgca ggttttccac ttgccagctg ccgtcggcgt tacgctgcgc
     8281 gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc
     8341 gttatcaata ctgccttcaa tcagtacatt ggtggcagga acatcattga gcgaaagcga
     8401 tccggcacta aactgaatct gcgccttcgt acccagcact ttaccggctt ttggtgacca
     8461 cggaactacg ccgccattta cccgctgcgc gctcagtttc cattcgctat tcgggctatt
     8521 aaacgccata tcacgcagtt gcagacgatc ggctttgaac ggtagcggcg cggtctggtc
     8581 ggtgagattc agcgtcccgt tttccagcag gatggtatcg acatggcgtg gttcggtcag
     8641 ttgccgactg cttagcgcaa tgtcgacact ttttgccacc agggtcgcgg gctggccatc
     8701 acggccaaac gtgacgttct ccagcacgat atgagatggc gcggaaaaac ggtgatccat
     8761 cgccccgaag gccagatgat agtcgctatt ctcggaaacc catgcgctga tatgttctgc
     8821 tccccagcgg gtttgcagaa gaaaatagag gccagcgatc accactaaca gagcgatgag
     8881 aatgtagaga agcagcttcc caataaattt catggtcttc catcccgtga aatgcacata
     8941 agggagttat gcacgattta cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac
     9001 tgcggcggta attatcttac cgccgccagt gaattactgt ttttctggtg ggaaaatcag
     9061 attcagcacg atggcagtaa taccgcccgc ggcgatcccg gaggagagca ggtttttcag
     9121 ccattcaggg gcaaactgca aaatcagcgg ctgctgagac acgcccagac caaccgccag
     9181 cgacagcgcg ataatcagaa tcgcccgacg gttcagcggt tcacgagaaa cgatacgcac
     9241 accggaggcg gcgatagtgc caaacattac aagcgttgcg ccgcccagaa ccggttctgg
     9301 aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc agcacgatca acatcagcgc
     9361 gacgacaaaa ccgacatagc ggctggcaac accagtcaac tggatcactc cgttgttttg
     9421 cccgaagcag gagttcggga aggtgttaaa caccgccgaa acaaacgagt tcaggccgtt
     9481 tgccagcacg ccgcctttca ggcgtttcat gtacagcgga ccggacactg gctgttcgga
     9541 aacgtcagag gtcgccgtga tatcgccaat ggtttccagc gaagtgatca taaagaccag
     9601 catcagcggc agcagcagac tccattcaat gccaagaccg taatagagcg gcgttggcac
     9661 cataatcagt tcttgcgtca tcggttcgtt gctttctggc aacatgccca taaaccacgc
     9721 cagcgcatat ccggccgcca tcgcaatcac cagtgaggcc acgcgtaagt aagggttacg
     9781 ttgacggtta agcaggataa ttaaggctaa gaccacgcct gccagcagca gatttttcgg
     9841 tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg ccaatggacg ttaacccaac
     9901 ctgaattagc gacaggccga taatcatcac cacaacgcca gaaaccagcg gcgtaataat
     9961 gcggcgcgcc agatgcagaa cgcgggagat caccatctcg gtgcaacttg ccagcatcaa
    10021 cgtgccgaac aaagccgcca tcatggtagg aacatcagca ccaccggttt tcagcgcggt
    10081 accgcccata atcagcgggg caacaaagtt gaagctggtg ccctgaatag acaacagccc
    10141 ggagccaacc ggaccccagg ccttaatttg aataatcgat gccacaccgg aggcaaacag
    10201 cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa cccagcgcct ggcagattaa
    10261 tagcgctggc gtgatcaccg caacgaacat cgccagcaga tgctgacagg cggcaaacag
    10321 ggtttgagga agcggcggac gatcttcaag acggtaaatc agttcgctgt tttgagtctg
    10381 cgcaaccggt tgcgcatttt ctgactcgag ggtggaaaca gacatcgaaa acaatcccgt
    10441 ggtagcaaag cgggcatttt agctgaccga aagagaaaag caaacgtttg ccacatcctc
    10501 atctaaatat ttaaatgaga taaatgcact tttttatata acttttggta tttttctgct
    10561 taaaatccat gccatttgtt cattaacagc tgagaatttg acccaggaaa gcataactcg
    10621 atacccgtca tagttcatga agttgccact gcgcgatgaa gtatgacgag tatgaaagag
    10681 tgatgcggat acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc
    10741 gcaacgctga cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa
    10801 tacaccatac cggaacctgt tgcgggtggt ttgttggtgg cgctggcgct actagtactg
    10861 aaaaaaagca tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg
    10921 gctttcttcg ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt
    10981 gtggttggca tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc
    11041 attggtatgg ctagcctgtt agggcttgat ccgctgatgg ggctgttggc cggttctatt
    11101 acgctttccg gcggtcacgg tacgggcgct gcgtggagta aattgttcat tgaacgttat
    11161 ggcttcacca atgcgacgga agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc
    11221 ggcttgattg gcggtccggt ggcgcgctat ctggtgaaac actccaccac gccgaacggt
    11281 attccggatg accaggaagt cccgacggcg tttgaaaagc cggatgtggg acgcatgatc
    11341 acctcgttgg tgctgattga aactatcgcg ctgattgcta tctgcctgac ggtggggaaa
    11401 attgttgcgc aacttttggc tggcactgct tttgaactgc cgaccttcgt ctgtgtcctg
    11461 tttgttggcg tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag
    11521 cgtgcggtat ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg
    11581 gggctgaaac tgtgggagct ggcttcgctg gcgctgccga tgctggcgat tctggtggta
    11641 cagaccatct tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac
    11701 tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg gcctcggtgc aacgccaacg
    11761 gcaatcgcca acatgcaggc gatcactgaa cgctttggcc cgtcgcacat ggcgtttttg
    11821 gtggtgccga tggtcggtgc gttctttatc gatatcgtca atgcgctggt gattaagctg
    11881 tatttaatgt tgccgatttt tgccggttaa aaatgaaaaa gcgtcggaag gaaccggaaa
    11941 cgctacgtga acactgtcgc catatttttg gtgatgagcc gccagtgctc tgtgtatggg
    12001 aaacggaatt tgattacgca gatgccgaac tcaaggcgct ggcggctaaa gagtggcagc
    12061 agatcagcgt atgggatttg agtgcttact atgtactcaa tctcgtttat aacgaaccga
    12121 tgcaaataga gttgtttcgc tatctgtttc cgctctgcct ggcgcaatgg catgaaaccg
    12181 ttttagctgg cggttatggt gatcacttcg aagaatcctt aatgaaggca ttgtgtcggc
    12241 cctatctctg gcaggagatg atgaatgctt cccaacgcca gcaggtacgg caatttttac
    12301 tggacaccgc gttgcagcgt atggataacg agcgtggttt caataacgtt ctttgctggc
    12361 tggcggtgtt caatactctg ggtggggccg cgccgttaat tcattctctc tggtcgcgct
    12421 ggtgggcgct ggatacgcca ggaaaagcgg tgtgtgctat tcagtatgcc gcgcatctaa
    12481 tttatcccat agaagccaat ccgttatggt cgcaagaatg gataggctgg gggcatccat
    12541 taggccataa agatggctgg tcctctgata atcgcgcttt tcttcgccaa atgttaacac
    12601 ctgaaatgat tgtggctggc gtacaggcag cggctgaaat tctgcgtggt gaaccggaag
    12661 gcgcaatggc ggcgcgaatt gcacaggatg cgtatgaggc aatggatatc ctcactatcc
    12721 agattgaaga tttactgcgt gacttgtctt gtgatgagtc tggtcatgcg ttggagtaac
    12781 gttccgtctc cggcatccag cgttctatca gggcttttgc ttgttgtggg taacgttcgt
    12841 gaatatgtcg cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg
    12901 ccactttaaa ctcagcatta cccgtctgac gcgtacctaa cagttcaccc ggtccgcgaa
    12961 tctctaagtc tttctgtgca atcacaaaac cgtcgttgct gtcgcgcagc acttgcagcc
    13021 gaatttgcgc ggttttggag agcggcgttt tgtagagcag cacgcagtga gaagccaccg
    13081 caccacgacc tacacgcccg cgaagctggt gtaactgcgc cagacccaga cgctccgggt
    13141 tttcgataat catcagactg gcgttaggca catcaacgcc gacttcaata acggttgtgg
    13201 caaccagcag gtgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcgacag
    13261 gtttcatccg cccgtgtacc aggccaacgt tcaactctgg tagcgccagt ttcaactctt
    13321 cccaggtagc ttccgccgcc tgcgcttcca gcaattccga ctcttcaatc aacgtacaaa
    13381 cccagtatgc ctgacgacct tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg
    13441 tacggcgggt atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat
    13501 ctatcaccga ggtatcgaga tcggcatacg cagtcattgc cagcgtgcgg gggatcggcg
    13561 tggcggtcat gatcaactga tgcggatgga agccctgctg ctggcctttc tcccacaatg
    13621 ccagacgctg atgcacgcca aaacgatgct gttcgtcgat aatcaccagc gccaggccgt
    13681 taaactgcac ctgttcctgg aagatggcgt gtgtaccgac aatcatctgc acctgaccgc
    13741 tggcgatggc ttcctgctgt gccagccgtg ctttaccttt ctgcttaccg gcgagccagc
    13801 ccacttcgat accgagcggt gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga
    13861 gtaattcggt tggtgccatc aatgctacct gtttgccgtg ggcaatcgca cgcaacgcgg
    13921 cgagggcggc gaccagcgtt ttaccggaac ctacatcgcc ctgcaccaga cgcatcatcg
    13981 gcacatccag cgccatatcg cgctcgatct ccgccactac gcgtgcctgt gcgcccgttg
    14041 gcttgaacgg taaggcggcg aggagtttat ttttcagcgt gtcattggcg ctcagcggct
    14101 gggcatgaaa acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca
    14161 gttcttccag aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct
    14221 gtagcgtcgg tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt
    14281 gtgacagttc cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct
    14341 ggtcggttaa tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg
    14401 tgagcgtttc ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga
    14461 tcatctccgc accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg
    14521 tcgccaggct atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg
    14581 aaccgtcgct gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca
    14641 gcacttcgcc ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat
    14701 gggtgcgatc ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt
    14761 tgattttcgc cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga
    14821 cagcatctaa caggcgacct ttcatggcac ttaccctgca gcctgcatag tagcccacca
    14881 gtcggcatca gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg
    14941 ttttgcgact ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc
    15001 ttccggcaac atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc
    15061 ttcgtaaaga atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat
    15121 cggaatgatg atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc
    15181 ctgtcccatc aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt
    15241 atcagaaaga tgggttgcca gaatctgcat gccctggcct ttgagatgag cgacggcatc
    15301 gccaatggtg cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc
    15361 catggtgcgc atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc
    15421 tgcggtacga ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt
    15481 cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat
    15541 aaaacattaa tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg
    15601 catgatattc gccagatgca cacggtcacg agcggtcaga cgaataaagg cgctgtagac
    15661 gcgaccatct ttctcttccg tattcaaact ttgaatattc gaggtcgtgg tgttaattgc
    15721 cgccgtcagg tttgccagcg caccctgatg attgaacatc tccaccttga tttcggtgat
    15781 gaactcctgc gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg
    15841 gtagccacgg atgttacggc aggattcatg gtggatcacc agacctttac cggggctgac
    15901 gtgggcgata atcgggtcgc caggaatggg gcggcagcat ttcgcaaagg tgatcagcac
    15961 gccatcagcg cctttgatcg gcagatgccc gtggctttga gtcgccggtg gaatggaggc
    16021 gtccccatgt tgtagatttt tcgcgaccac cacgctcatt gcgttaccaa ggccgatttc
    16081 tgccagcaga tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc
    16141 ctgcgggatt tcattcagct tacggctacc acccaaagca tggttgagca gacgacggcc
    16201 caggcttaca gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc
    16261 ttttgagcta acgacaaagt tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat
    16321 gatttcaacg gtttgaccgc tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac
    16381 gcgtgcgccc acgcaggcat gaccgatatc ggtatgcact gcataagcga agtcgacggg
    16441 cgttgcaccg gcaggcagct cgacaattcg cccttccggt gtgaaaacgt aaatctcatc
    16501 cgggaagaga tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg
    16561 cagctccagc aggctttgca tccagcgctg ggcgcggatt tgtgcggtag tactggtttc
    16621 gccgtgctct ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc
    16681 ttcggtacgg atctggacct caaccggcac gccgtgcggg ccaatcatcg aggtgtgcaa
    16741 cgactgatag ccgttcgctt taggaatggc gatatagtct ttcacgcgac ccggacgcgg
    16801 cttgtacagg ctatgcattt gcccgagcac gcgataacag gtgtcagaat cattgacgat
    16861 cacgcggaaa gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt
    16921 gcagtaaatc gaataaagat gcttttcgcg accactgacg cggcacggta ttcccgcttc
    16981 ctgcaaacgc ccttcgattt cagagaggat tttttggatc atctctttac ggttgccgcg
    17041 cgcggctttc accacttctt taattacgcg gtaacgattg ggatacagcg cctcaaaacc
    17101 cagctcttcg agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctata
    17161 aatttcgaga gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt
    17221 gcgcatgttg tgggtacggt cggcaagttt gatgagaatg acgcggatat cctgcaccat
    17281 cgccataatc atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag
    17341 cttatcaagt ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc
    17401 catgtcctgg taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag
    17461 cgtttcatag tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat
    17521 atagggttca ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc
    17581 ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct
    17641 ttcaaacaga tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt
    17701 aacggcttgt aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag
    17761 gatctggttg ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc
    17821 gttttcttcc ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc
    17881 ggcgaccagt accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac
    17941 gcgtgccata cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca
    18001 ggttcagtct gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat
    18061 gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc
    18121 attcacaatc agataatcat attcggcgta atggctcatt tctgcaacag cttgcgccat
    18181 acgctttgca atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc
    18241 aattttggac ggcggtaaaa taaagatact ccgcgcgtgc ggcattttct ggcgaatttg
    18301 ctgcgcgccc tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc
    18361 aatggcctca cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc
    18421 atctctgcta atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg
    18481 gacttcacca ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa
    18541 cggttgggtt tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga
    18601 aacaatataa agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag
    18661 cctacatacg agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt
    18721 ttgcgcaaag ttgttgcgtt ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa
    18781 taaagacgat tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt
    18841 caccacgttg tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg
    18901 cgatattaat aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt
    18961 cgccagccag agcacaggaa gatatttccc gcctgcaaca gcaaataaaa cagtgggacg
    19021 atgactactg gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg
    19081 cccgtcttac gcagtggcaa cgctgctttg ggagcgagcc ccgcgacgtg atgatgccgc
    19141 ctttaaatgg tgcggttatg catccggttg cccatacggg tgtacgcaaa atggtggata
    19201 aaaacgcatt aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg
    19261 atggcgttgc ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg
    19321 gtaatggcct gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgccgc
    19381 aaaccgttag cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg
    19441 aggggcatat ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcctga
    19501 tgatgcgcca ggacgatagc gacacgctga attctctggg cgtttttgtc tgggcatggc
    19561 cggatggacc gcagttaatg accgatcgtt taaaggagct ggctaccgca ggttttactc
    19621 taacgcagag gtatacccgt gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg
    19681 agtggtggaa agcgaaatta cccttcgtca ccgatggcgt ggttgtgcga ggggcgaaag
    19741 aacccgaatc ccgccattgg ctaccaggcc aggcagaatg gctggtggcc tggaaatatc
    19801 aacctgtagc tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta
    19861 aaatatcggt ggttgcgtca ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg
    19921 tgaatattgg ttccgtcagg cgctggcagg agtgggatat tgcgcctggt gatcagatcc
    19981 tcgtcagcct tgccggtcag ggtattcctc gcattgatga tgtggtctgg cgcggtgcag
    20041 aacgtacaaa accgacacca ccagaaaacc gctttaactc gttgacctgc tactttgctt
    20101 ctgatgtttg tcaggaacag ttcatttcac gcttagtctg gctggggtca aaacaggttc
    20161 tggggctgga tggcattggt gaggccggct ggcgcgcgct gcatcagact catcgctttg
    20221 agcatatctt ttcctggctt ttattaacgc cagagcaatt acagaacacg ccggggatcg
    20281 cgaaaagtaa aagtgcgcag ctatggcatc agtttaatct ggctcgtaag cagcctttta
    20341 ctcgctgggt gatggcaatg ggaataccgc taacccgggc ggcgcttaat gcccgtgatg
    20401 aacggtcctg gtcgcaactt ttatttagca cggagcagtt ctggcagcag ctgccgggga
    20461 ctggatcggg gcgcgccaga caggttattg aatggaagga aaatgcgcaa atcaagaagc
    20521 tgggcagttg gctggctgcc cagcagatca caggttttga accttagtgt ccttcgtggc
    20581 tgtagtaaaa gaccggtaat cccagcttaa ggcgtagcgc cagtaagcgg gcaaaaaagc
    20641 cgaataccag ggtggagata attaccacat catggttaga aacatagtgt tgcagcgcga
    20701 tgtacagcac ggcggaggca aatgagacac cggcatacaa ctctttctgg aataccagtg
    20761 ggatacgttt acagaacata tcgcgcaaaa cgccgccaaa cacgccggtg gttaccgccg
    20821 cgacaacggc aataattggg ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag
    20881 aaaagaccac cagtccaagc gcatcgagca ccagaaacac tttgcgcagg taaggcatta
    20941 caggagcaac gatagtggtg agtactgcgg cggtagcgac gataatcaca tattccgggt
    21001 gtttgaccca gccgagcgga tagtggccca gcagaatatc gcgcactgac cctccgccaa
    21061 ttgcggtggc ggtcgcaata ataattacgc caaatgtatc catgcggcgt cgcccggccg
    21121 ccagagcccc cgtcatggct tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca
    21181 tggttctctc cctaaaaatc tgtctggcag ggtagcgatt tgtgcgtatt gtcacgattg
    21241 agatttttta aggtgagagg aaattgataa cttgtagtta tgtgaaatcg ctattttctg
    21301 tagcgtagat gcattcttct gacttcttgt ctgagaaaat gcataagaaa aaataggtta
    21361 aaattgaggg gtggaaaaga aaggtgcaat gtttagccat tgtgttcacc gcacctggac
    21421 tttggcttac gcatcgggta tctcaatcaa caaaaagagt ttttagtttc tgctatttct
    21481 tctctgtaat ttttacactg ttttccaact gcttgatgct gacctgcggg gtcggcaact
    21541 cctcaggcat ggttccgcca agttcctgaa tggtttgcct caccttgcta cccacgtcaa
    21601 agtgtgtggt attagcttgc tgttttgaat tcacgccatc ccgcttgagt ttttcttctg
    21661 tttgggtagc tcgaaagaga ttagccgcca gttctgttga acccatatga tccaggatct
    21721 tctgactctt tttcagcccc ttccgctgat ggatggcttt ctgatctaat ccaccataca
    21781 gcccccggta accatgattc tgaaatatgg cgaaatcgat agctgtcgct accccagctt
    21841 gctgcgctgc ctcaaccaat tgtttgttat gttctttcaa ttcatttctc agaaaaagac
    21901 gcttttcgtc ttcacgaagt tgcctgaaag cttcatcgtc agccagctcc tgccgtcggg
    21961 tctggatagc aaaataagtt tgccctgccg caataaccgg tttcgcaggg tcgccgtttt
    22021 gtactaccag gtaacaggca tagcgggaga gatgaacatc ttcaagttct cgttgagccc
    22081 ctgaacctaa tacaaccatt ttggtggttt caacgaaatg gtcagatgca gcttgattgc
    22141 tggcttcgca cgcttgagtc gcgcgggcca gtaccttttg aaaattgcgc cagtcccgat
    22201 aatccagtaa cggtgccaat tctctagctg accaaaattc ttgcccttca gtgccataat
    22261 gccgtatttc ttcaaaaggt tgatgatgtt cgttcattgc ccactccatg tgatatcgat
    22321 tgcaaagaaa gtaaatctgt aactgtattt atatacagtt gtgagtatct cacgttctca
    22381 gtgtgctggg caaaaaaaca tcgcaaaatc tggagcaggc acgcaaaaca gaattgaagt
    22441 tgcggggaag aaaaatgccg ctctgtttga gtgggcggca tttgtgaagg ctgaaaagta
    22501 agaattggcg ttacgagtta ttcaatgttc tgaatctgct cgcgcatctg ctcaatcaac
    22561 actttcagct caatcgcgga gtttgtcact tcggcattga tagacttcga cgcaagagtg
    22621 ttcgactcgc ggttgaattc ctgcatcata aagtccagac gacgaccaac ggcttctttt
    22681 ttcttcagaa tgttgtaggt ctctttgaca tgcgcttcca ggcggtccag ttcttcggca
    22741 acgtcaattc gttgtgccag cagaaccagc tcctgctcca gacggttgtt ttccaactga
    22801 acctgagcat cttccagctt cgcgaccaga cgctcacgct gccattgcag gatttccggc
    22861 atatgggcgc ggactttgac cacttcggcg gtgacgcctt ccagacgctg ctcgatcaat
    22921 gctttcagtg cctgaccttc ggtttcgcgc gcgacaataa agtcgtccag cgtaccatcc
    22981 agcgccgcga gaatttcagc ggcaatggcg tcaagatcct gctcctgggc tgccatcacg
    23041 cccggccagc gtagaatatc aaccgggttg atttcccctt cgtcactctg catttttacc
    23101 cagttcgcgg cagttaccag ctgtttagcc agtttttcgt tgaggatcag ctcaccttgc
    23161 gcgctaacat ctggctcata gcgcagggta cattccactt taccgcgcgt caggcgagaa
    23221 cgaatacgct cgcgaacgac aggttcaagg ctacggaact gctccggcag acgaaagtaa
    23281 gtttccagat aacgctggtt taccgagcgc atttcccagg tcgcgctccc ccattcaccc
    23341 ttgatttcac gccgggcgta ggcggtcata ctgcggatca tagacgttcc tgtttataaa
    23401 aggagaggtg gaaggattat agccatcgat gccttgtcag gataggaata accgccggaa
    23461 gtccgtataa tgcgcagcca catttgtttc aagccggaga tttcaatatg cgtccagcag
    23521 gccgtagcaa taatcaggtg cgtcccgtta ccctgactcg taactataca aaacatgcag
    23581 aaggctcggt gctggtcgaa tttggcgata ccaaagtgtt gtgtaccgcc tctattgaag
    23641 aaggcgtgcc gcgcttcctg aaaggtcagg gccagggctg gatcaccgca gagtacggca
    23701 tgctgccacg ttctacccac acccgtaacg ctcgtgaagc ggcgaaaggt aagcagggtg
    23761 gacgcacaat ggaaatccag cgtctgatcg cccgtgctct tcgcgcggca gtagatttga
    23821 aagcgctggg tgagttcacc attacgctgg actgcgacgt gcttcaggct gatggtggca
    23881 cgcgtaccgc gtcgattacg ggtgcctgcg tggcgctggc agatgcgcta cagaagctgg
    23941 tggaaaacgg caagctgaaa accaacccga tgaaagggat ggtagccgca gtttctgtcg
    24001 gtattgtaaa cggtgaagca atttgcgatc tggaatacgt tgaagactca gccgcagaga
    24061 ccgacatgaa cgtagtgatg accgaagacg ggcgcatcat tgaagtgcag gggacggcag
    24121 aaggcgagcc gttcacccat gaagagctac tcaccttgtt ggcgctggcc cgagggggaa
    24181 tcgaatccat tgtagcgacg cagaaggcgg cgctggcaaa ctgattttta aggcgactga
    24241 tgagtcgcct tttttttgtc tgtagaaaag taagatgagg agcgaaggca tgaaaccata
    24301 tcagcgccag tttattgaat ttgcgcttag caagcaggtg ttaaagtttg gcgagtttac
    24361 gctgaaatcc gggcgcaaaa gcccctattt cttcaacgcc gggctgttta ataccgggcg
    24421 cgacctggca ctgttaggcc gtttttacgc tgaagcattg gtggattctg gcattgagtt
    24481 cgatctgctg tttggccctg cttacaaagg gatcccgatt gcgaccacta ccgccgtggc
    24541 gctggcggag catcatgacc ttgacctgcc gtactgcttt aaccgcaaag aggcaaaaga
    24601 ccacggtgaa ggcggcaatc tggttggtag cgcgttacaa ggacgcgtaa tgctggtaga
    24661 tgatgtgatc accgccggaa cggcgattcg cgaatcgatg gagattattc aggctaatgg
    24721 cgcgacgctt gctggcgtat tgatttcgct tgatcgtcag gaacgggggc gcggcgagat
    24781 ttcggcgatt caggaagttg agcgtgatta caactgcaaa gtgatctcta tcatcaccct
    24841 gaaagatctg attgcctatc tggaagagaa gccggaaatg gcggaacatc tggcggcggt
    24901 taaggcctat cgcgaagagt ttggcgttta aagaaactcg ccggatgaaa agtcatccgg
    24961 cgtcatatta ctgcaactgc gcggcaatta gcggccagcg ggcgtcaaaa tcatccgtcg
    25021 ggcggtattt aaattcgctg cggacaaaac gtgacagcat accttcacag aaggccagga
    25081 tctggcttgc cagcagggtt tcatcggtgg tgtaaccttc accctcacgc attctctttt
    25141 cacgcaatac ctggcgcagc tgcgcttcaa tacgctcgaa cagctggttg atacgccctt
    25201 gcaggcgatc ctgttcaaac atcagcgcat gaccagtgag gatgcgggtc aggccaggat
    25261 tacgctcacc aaaaccgaga agcagcaaca caatcagacg caggcgcgct gtggtgtctt
    25321 tctcatcttt cagaatcagg ttgatgcgag taatcaggct atcttcgata aactcaatca
    25381 ggctatcgaa catgcgggtc ttactgggga agtggcgata cagtgccgct tcggaaacgc
    25441 cgacagaggc ggccagtttt gccgtcgtga tacgttggct tccatcgctg gattccagca
    25501 tcagcgccag agactgaagt atttcctcgc gacggttcct tttcgcagtt tgtttttctg
    25561 ccatgttaca aaatacccct gaaaataagc acttgccagg cggcacccac gctatgaccg
    25621 caaacgaaat gtttgcggct atgttatgac gttattcgga tgcgtatgtg ttactgacga
    25681 ccagagtgac caaagccgcc ttcaccgcgg tcggtggcgt cgaaatcttc caccagatta
    25741 aattcagcct gtactaccgg aacaaaaatc atctgggcga tgcgttcgcc aggttgaatg
    25801 gtgaagctgt cctgaccacg gttccacacg gaaatcatca actggccctg atagtcagaa
    25861 tcgatcaatc ctaccaggtt accaagcacg ataccgtgct tatgtcccaa tccggagcgc
    25921 ggcagcatca ttgccgccag tgaaggatcg gcaatatgaa tcgccagccc ggtcggaacc
    25981 agcgtagtgt cacccggagc cagttctacg gcgtcgtcga gacaggcacg caggtcaagt
    26041 ccggcagagc cagaggtggc ataagtcggg agcggaaatt ccttcccaac gcgcgggtcc
    26101 agaatcttaa cgtcgatttt tttcatcata acgggtcacg atctcgtcga gtaataattg
    26161 gccaaggagc tctttgcgct caagcggtaa gactttatct ccgtcctgcc agaaaaggtg
    26221 taatgcgttg ttgtcgctgt taaatccttg agttggctgg gaaacatcgt tcgcgcagat
    26281 cagatcaagg tttttacgga tacgtttttg ccgggcgtat tcttccacat tatttgtttc
    26341 ggcggcaaat ccaacgacgt agggtcgatg gtcttttagt gcggcaacgc ctgcgacgat
    26401 atcggggttt ttaaccattt ttattgttaa ttcatcaccc tgcgtggcct gctttttgat
    26461 tttctctggg gccacggtag ctgcgcgata atccgccacg gcggcgcagc cgataaaaat
    26521 attttgctgc tgtacagaag cattcacggc ggcttccatt tccagcgcgg tcatcacatc
    26581 aacacgttta acaaacggtg gcgtcggtag tgaaaccgga cctgatacca gcgtgacgtt
    26641 cgcgccacga cgggcagcgg cggcggcgat agcaaaaccc atcttgccgg agctgtgatt
    26701 agagatataa cgcacgggat cgagcggttc acgcgtcggg ccggcggtaa tcataatgtt
    26761 cagatgtttc aggtcgttga cgggcgaaaa atgcgctacc gccatatcca caatggttaa
    26821 cggatcgagc attcgcccag gaccgatatc accacaagcc tgactgccac tgtctggccc
    26881 ccagatgagc aaaccacggg aagcaagcac ctctaaatta tgctgcgtgg cagcggcacg
    26941 gtacatctgc tggttcatgg cggggagcac ggctacaggc gcaggtgtag ccagacaaat
    27001 cgtcgatacc aggtcattcg ccattccggc agcaacacgg gcaatcaaat ctgccgtggc
    27061 aggggcgaga atcactaaat cagcccattt acccagctca atatggccca tagcggcttc
    27121 ggctgccggg tccagcagac tgtcggaaac gggataacca gaaaccgcct gcaagctaag
    27181 tggggtgata aaggcttttg ccgcttcggt catggctacg cggacgtcgg ccccgcgatc
    27241 gcgcaaacga cgcaccagtt caggggtttt ataggcagca ataccgccgc taacgccgag
    27301 aacgattttt ttaccggcca ggctcatgat gatttttcct gttgggtgac accagaagtt
    27361 ggcgatttta tcacaatact tttgttgtcg tgccttcacc tgagaatcac tttgcgaggc
    27421 gctttccagg attgaaaact ggccgtcgat ttaacggaac ggctatgaca ggatgcgagc
    27481 accacaaagg aggtgaaggt gaaaaacaat tcacagctgt tgatgccgcg cgaaaaaatg
    27541 ctgaagtttg gtattagcgc cttaacggat gtcgagctgc tggcgctatt tctgcgtacc
    27601 ggaacgcgcg gtaaagatgt attaaccctg gcaaaagaga tgctggagaa tttcggctct
    27661 ctttatggct tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgtg
    27721 gcgaaatttg cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg
    27781 atgcgtgaag aaagcccttt actcagcccg gagatgacga gggaattttt acaaagccag
    27841 ctcacgggtg aggagcggga gatctttatg gtgatctttc tcgactccca acaccgggtt
    27901 ataacgcata gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa
    27961 attatccgcg aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct
    28021 tcgggttgtg ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt
    28081 tgtcagttca tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt
    28141 tcttttgccg aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct
    28201 gttcgggact tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg
    28261 gtttggcatt tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa
    28321 agcctgacga ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata
    28381 gacatgtccc gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc
    28441 cacgcactga acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg
    28501 gttgagagcg agaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc
    28561 gataaaaaag gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa
    28621 gtacttagag gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt
    28681 ctgctggtac tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac
    28741 tggaactgaa aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa
    28801 tcaaataatt ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc
    28861 tgcttgcccc catattgact gcatctgttc attcctggag atgctatgcc tgaattaccc
    28921 gaagttgaaa ccagccgccg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat
    28981 gcggtggtgc gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc
    29041 gaccaaccag tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga gctgcctgag
    29101 ggctggatta tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt
    29161 ccccctgaaa agcatgacca tgtggatttg gtgatgagca acggcaaagt gctgcgctac
    29221 accgatccgc gccgctttgg tgcctggctg tggaccaaag agctggaagg gcacaacgtg
    29281 ctggctcatc ttggaccgga gccgcttagc gacgatttca atggtgagta tctgcatcag
    29341 aagtgcgcga agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta
    29401 ggggtaggga atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg
    29461 ctggcgtcat cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg
    29521 ttgctgcgtt cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt
    29581 aaaccgggct atttcgcaca ggaattgcag gtttacgggc gaaaaggcga gccgtgtcgg
    29641 gtgtgcggta cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg
    29701 cagtgccaga agtaattcat gcgcgccgga tggcatacca tccggcataa acgctacgct
    29761 aacttcgcca tcagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga
    29821 tggcgcgcca cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc
    29881 agaaacacac tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca
    29941 tattcaaaat ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg
    30001 gcgaagttcg ccatcaaatc actaaacccg accacttcca cgttccccag atgcgcggtt
    30061 gcctgctgtg ccagtgccac acgctcttcc agggtaaaca tcggtttttt actggggctg
    30121 gcggcaatcg ccagaataac gtgatcgaac atctgcgtgg cgcgcgtcac gatatcgata
    30181 tgaccattgg taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc
    30241 tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc
    30301 ctggttttga tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc
    30361 ggtgagtaaa gaggacacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc
    30421 gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta aaagtatgcg gccccatcaa
    30481 taccggaata gcgtgtgcgg cagcttccag cggattatgc ccaccacgtt caaccagtga
    30541 accgccaaca aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc
    30601 gccaactaca acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct
    30661 tagtccagcc tggcggacaa gattaatcgc atccgggaag cgttctggat gacggggtac
    30721 caggatgagc aataaattcg ggaattgctg taacaatgcc tgatgtgcgg cgatcaccac
    30781 actctcttcg ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccactg
    30841 gcggcgcagc gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag
    30901 gctaccggta acggtcacct gattattttt tgcgcccagc gccacaaaac gtgcaccatc
    30961 ttcttcattt tgcgcagcaa tcagcgtaat acgacgcagc aagcgacgga cgaatttacc
    31021 cagtttggca taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg
    31081 aattttacgt ttgtgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa
    31141 caccagtttg gggtcgactt tattcaggaa acggttgagc gcatcgggca ggtcatacgg
    31201 cagataaacg tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg
    31261 cgtcatggtt gtaacggtaa tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa
    31321 cgggatcgcc gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt
    31381 tagcggatgg cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt
    31441 acgtccgcgc acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt
    31501 gtaaagcaat tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat
    31561 ttagctgtgg ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca
    31621 attcttgctg aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt
    31681 acgtgcagga aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt
    31741 gattgatttc tttattgaaa atcaacaatg tgcttaaaaa tcattcgggt cggattgccg
    31801 cgaaagaagt gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt
    31861 tgagcctggg gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa
    31921 agagttactt gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg
    31981 ggatatgtta ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa
    32041 aatcgatgtg ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa
    32101 cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt
    32161 tcatctcatc aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca
    32221 atggatggtt gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta
    32281 tcatcatcgg cagtctgctt tttggcgtaa aagtttcacc catttggtgc cgttgcaggg
    32341 tggaaatgtg gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt
    32401 gaagcagaca accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga
    32461 tcacgctggt gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa
    32521 atgctgggac aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga
    32581 agtcgttctg acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca
    32641 gggatgccag acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg
    32701 tgcgttaatc gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc
    32761 cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg acagatcata ttttctggcg
    32821 tccctggtca aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg
    32881 cgatcagcgt gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc
    32941 tgctgtcgat aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt
    33001 tgtttatata aatattttcc ctttggcggt ttgcagcgtg attttatgcg tattgctcag
    33061 acagtcgccg cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc
    33121 cctgatgtat ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat
    33181 gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt
    33241 ggattcaaca aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag
    33301 aaagtagcgc aggaaaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgcc
    33361 gcctttgagc gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat
    33421 aagcaaatcg ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg
    33481 ccaccgggga tttatcctga tcgtaaatat agccagcagc cagccaatag ccgtgaaatc
    33541 ttccgtaaga agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac
    33601 ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg
    33661 cgccacaaca cattgctata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg
    33721 gcagaaaaac gcggcgtgcg cagtaatgtt cactttttct cggggcgcaa tgatgtctcg
    33781 gaattaatgg cggcggcgga tttattactg catcctgcct accaggaagc ggcgggaatt
    33841 gtgctgctgg aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat
    33901 gcgcattata ttgtcgacgc taattgcggc gaggctattg ctgagccatt ccgccaggaa
    33961 acattgaatg agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg
    34021 gaaaatgcgc gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg
    34081 gatatcataa cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg
    34141 cggcaaagat ccttttgagg aagttaaaac cttgcagggt gaggtatttc gtgaactgga
    34201 aactcgccgt actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg
    34261 cggcacgacc ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg
    34321 cgctgaccgc gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta
    34381 tggggtggca tttggcgaaa aaggcatgaa tccgctgacc agaacttcat ttattattac
    34441 cgaagatctg acaccaacca taagtcttga agattacagt gctgactggg cgactaaccc
    34501 tccagatgtt cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat
    34561 gcatgctgcg ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc
    34621 tttttccggt aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct
    34681 tcgcacgcgc gttccacgtc gttggcggga taaagatctt attgggcttt atttttcttc
    34741 gatgaatatc ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgccgc
    34801 cccgcttaaa gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc
    34861 cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa cccgtaactt atttttgcca
    34921 aaattttgga tacagaataa atatgtctca actcaatgat agtgacatca tcctttttga
    34981 gtataatttt cattatcaaa atataagatc taaaaatact cttgatatag catttggtat
    35041 tgacagaaat tttctttttg gatgtggtgt agccatcgca tctattctat taaacaatag
    35101 agaaatctc
//
LOCUS       DAZFVO020000033        35051 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFVO020000033 DAZFVO020000000
VERSION     DAZFVO020000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 35051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.033"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(56..724)
                     /gene="ydcL"
                     /locus_tag="ACHKNB_RS07590"
                     /old_locus_tag="pgaptmp_001518"
     CDS             complement(56..724)
                     /gene="ydcL"
                     /locus_tag="ACHKNB_RS07590"
                     /old_locus_tag="pgaptmp_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HGU4195060.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="ACHKNB_RS07595"
                     /old_locus_tag="pgaptmp_001519"
     CDS             complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="ACHKNB_RS07595"
                     /old_locus_tag="pgaptmp_001519"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HGU4195061.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="ACHKNB_RS07600"
                     /old_locus_tag="pgaptmp_001520"
     CDS             complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="ACHKNB_RS07600"
                     /old_locus_tag="pgaptmp_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HGU4195062.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2733..3713
                     /gene="ydcK"
                     /locus_tag="ACHKNB_RS07605"
                     /old_locus_tag="pgaptmp_001521"
     CDS             2733..3713
                     /gene="ydcK"
                     /locus_tag="ACHKNB_RS07605"
                     /old_locus_tag="pgaptmp_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HGU4195063.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHALVEAFDGDTVHVRGPKVINGEGRITRTPLAGLL"
     gene            complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="ACHKNB_RS07610"
                     /old_locus_tag="pgaptmp_001522"
     CDS             complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="ACHKNB_RS07610"
                     /old_locus_tag="pgaptmp_001522"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HGU4195064.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTVEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4307..4531)
                     /gene="ydcH"
                     /locus_tag="ACHKNB_RS07615"
                     /old_locus_tag="pgaptmp_001523"
     CDS             complement(4307..4531)
                     /gene="ydcH"
                     /locus_tag="ACHKNB_RS07615"
                     /old_locus_tag="pgaptmp_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HGU4195065.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="ACHKNB_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /gene_synonym="mdoD"
     CDS             complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="ACHKNB_RS07620"
                     /old_locus_tag="pgaptmp_001524"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HGU4195066.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6551..7894)
                     /gene="ydcJ"
                     /locus_tag="ACHKNB_RS07625"
                     /old_locus_tag="pgaptmp_001525"
     CDS             complement(6551..7894)
                     /gene="ydcJ"
                     /locus_tag="ACHKNB_RS07625"
                     /old_locus_tag="pgaptmp_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4195067.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8111..9034
                     /gene="ydcI"
                     /locus_tag="ACHKNB_RS07630"
                     /old_locus_tag="pgaptmp_001526"
     CDS             8111..9034
                     /gene="ydcI"
                     /locus_tag="ACHKNB_RS07630"
                     /old_locus_tag="pgaptmp_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4195068.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9072..10712)
                     /gene="trg"
                     /locus_tag="ACHKNB_RS07635"
                     /old_locus_tag="pgaptmp_001527"
     CDS             complement(9072..10712)
                     /gene="trg"
                     /locus_tag="ACHKNB_RS07635"
                     /old_locus_tag="pgaptmp_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HGU4195069.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11111..11260
                     /gene="hokB"
                     /locus_tag="ACHKNB_RS07640"
                     /old_locus_tag="pgaptmp_001528"
     CDS             11111..11260
                     /gene="hokB"
                     /locus_tag="ACHKNB_RS07640"
                     /old_locus_tag="pgaptmp_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HGU4195070.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11238..11306)
                     /gene="yncP"
                     /locus_tag="ACHKNB_RS07645"
                     /old_locus_tag="pgaptmp_001529"
     CDS             complement(11238..11306)
                     /gene="yncP"
                     /locus_tag="ACHKNB_RS07645"
                     /old_locus_tag="pgaptmp_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HGU4195071.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(11332..11505)
                     /locus_tag="ACHKNB_RS07650"
                     /old_locus_tag="pgaptmp_001530"
     CDS             complement(11332..11505)
                     /locus_tag="ACHKNB_RS07650"
                     /old_locus_tag="pgaptmp_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195072.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="ACHKNB_RS07655"
                     /old_locus_tag="pgaptmp_001531"
     CDS             complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="ACHKNB_RS07655"
                     /old_locus_tag="pgaptmp_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HGU4195073.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12469..13470
                     /gene="gap"
                     /locus_tag="ACHKNB_RS07660"
                     /old_locus_tag="pgaptmp_001532"
     CDS             12469..13470
                     /gene="gap"
                     /locus_tag="ACHKNB_RS07660"
                     /old_locus_tag="pgaptmp_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4195074.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="ACHKNB_RS07665"
                     /old_locus_tag="pgaptmp_001533"
     CDS             complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="ACHKNB_RS07665"
                     /old_locus_tag="pgaptmp_001533"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HGU4195075.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15148..15948)
                     /gene="ydcF"
                     /locus_tag="ACHKNB_RS07670"
                     /old_locus_tag="pgaptmp_001534"
     CDS             complement(15148..15948)
                     /gene="ydcF"
                     /locus_tag="ACHKNB_RS07670"
                     /old_locus_tag="pgaptmp_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HGU4195076.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="ACHKNB_RS07675"
                     /old_locus_tag="pgaptmp_001535"
     CDS             complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="ACHKNB_RS07675"
                     /old_locus_tag="pgaptmp_001535"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HGU4195077.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20323..20928
                     /gene="azoR"
                     /locus_tag="ACHKNB_RS07680"
                     /old_locus_tag="pgaptmp_001536"
     CDS             20323..20928
                     /gene="azoR"
                     /locus_tag="ACHKNB_RS07680"
                     /old_locus_tag="pgaptmp_001536"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HGU4195078.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20979..22295)
                     /gene="ynbD"
                     /locus_tag="ACHKNB_RS07685"
                     /old_locus_tag="pgaptmp_001537"
     CDS             complement(20979..22295)
                     /gene="ynbD"
                     /locus_tag="ACHKNB_RS07685"
                     /old_locus_tag="pgaptmp_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HGU4195079.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(22285..24042)
                     /gene="ynbC"
                     /locus_tag="ACHKNB_RS07690"
                     /old_locus_tag="pgaptmp_001538"
     CDS             complement(22285..24042)
                     /gene="ynbC"
                     /locus_tag="ACHKNB_RS07690"
                     /old_locus_tag="pgaptmp_001538"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4195080.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24058..24954)
                     /gene="ynbB"
                     /locus_tag="ACHKNB_RS07695"
                     /old_locus_tag="pgaptmp_001539"
     CDS             complement(24058..24954)
                     /gene="ynbB"
                     /locus_tag="ACHKNB_RS07695"
                     /old_locus_tag="pgaptmp_001539"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4195081.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24954..25559)
                     /gene="ynbA"
                     /locus_tag="ACHKNB_RS07700"
                     /old_locus_tag="pgaptmp_001540"
     CDS             complement(24954..25559)
                     /gene="ynbA"
                     /locus_tag="ACHKNB_RS07700"
                     /old_locus_tag="pgaptmp_001540"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4195082.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(25730..28036)
                     /gene="ydbD"
                     /locus_tag="ACHKNB_RS07705"
                     /old_locus_tag="pgaptmp_001541"
     CDS             complement(25730..28036)
                     /gene="ydbD"
                     /locus_tag="ACHKNB_RS07705"
                     /old_locus_tag="pgaptmp_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HGU4195083.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="ACHKNB_RS07710"
                     /old_locus_tag="pgaptmp_001542"
     CDS             complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="ACHKNB_RS07710"
                     /old_locus_tag="pgaptmp_001542"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HGU4195084.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="ACHKNB_RS07715"
                     /old_locus_tag="pgaptmp_001543"
     CDS             complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="ACHKNB_RS07715"
                     /old_locus_tag="pgaptmp_001543"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HGU4195085.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="ACHKNB_RS07720"
                     /old_locus_tag="pgaptmp_001544"
     CDS             complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="ACHKNB_RS07720"
                     /old_locus_tag="pgaptmp_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HGU4195086.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(30814..32127)
                     /gene="paaK"
                     /locus_tag="ACHKNB_RS07725"
                     /old_locus_tag="pgaptmp_001545"
     CDS             complement(30814..32127)
                     /gene="paaK"
                     /locus_tag="ACHKNB_RS07725"
                     /old_locus_tag="pgaptmp_001545"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HGU4195087.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="ACHKNB_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="ACHKNB_RS07730"
                     /old_locus_tag="pgaptmp_001546"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HGU4195088.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="ACHKNB_RS07735"
                     /old_locus_tag="pgaptmp_001547"
     CDS             complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="ACHKNB_RS07735"
                     /old_locus_tag="pgaptmp_001547"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HGU4195089.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(33771..34586)
                     /locus_tag="ACHKNB_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /pseudo
     CDS             complement(33771..34586)
                     /locus_tag="ACHKNB_RS07740"
                     /old_locus_tag="pgaptmp_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
     gene            complement(34695..>35051)
                     /locus_tag="ACHKNB_RS07745"
                     /old_locus_tag="pgaptmp_001549"
     CDS             complement(34695..>35051)
                     /locus_tag="ACHKNB_RS07745"
                     /old_locus_tag="pgaptmp_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195090.1"
                     /translation="QLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESIFYELDG
                     EWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLNTKLFKVQ
                     PYPKPQTVKTGLKESE"
BASE COUNT         9048 a         9195 c         8070 g         8738 t
ORIGIN      
        1 ggggttttac ggcgcaccgg ataaaaagcg ccatctaacg atggcgcgtc tggaactact
       61 ttttgttaac gtcaaacatg gtggcatcgg tcgccatgtc atcaataact tgtttaatat
      121 tttcgaaagc cattggcgta ctttcgttat tcaggtcttt accttcgccc tgacgaacga
      181 ctttgataac cggtttatta gtcgctgcat caatcagctc accttcaaaa tagaggcgag
      241 tatccatggt acggtggcct gtagccattt gcgtccccgc aaccactaat gcaacaggaa
      301 ccacttcata gaattgcagc ccttctttgc tggtatctac accggtaatg gccccacgga
      361 aaatcagact acgcggccca gcggtggtaa ccagtggttt acgctgcgct atcgcttctt
      421 tcatttcggt gttggtatag ttcaaaattt tatccagaac tttctgccct acctgggtag
      481 aaggttttgg taccggataa taagtgattg ggttccagac gatgctgtca tatttgcttt
      541 gatcaaaact cgggtctacc caacgtaaaa caggtttacc tgttgctgag gttgtttctt
      601 ttaaatcaga gtaattgttt aaaaaaccag aatatttatc tggctgggtg attttagagg
      661 cacaaccaga cagagccaac aagccgcata aagctgcaac tttcgcaaat gatgtggtac
      721 gcatgaatag tttcctgaga tgattatatt actttctaag ttatagcaaa aagcgccgct
      781 tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt tcagcccctt ttcagacgac
      841 atagtcaatg ccgaaaaagg ggctaagtca atcatcagta gttaaatttc agtaagttaa
      901 gctgctaaaa aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct
      961 taccgaaaag aagaacagta aatcatctca gcgtggggaa ttttcacgcg ccttattctt
     1021 agcgggtcat tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt
     1081 ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat ttcaccctct cccagccttc
     1141 gtaatatcga cgtaattctc cctctttgaa ggcaaacggg aagccgacgg tacatggata
     1201 atcagcggta tccatcgccg ccacaatcag gttgtaacca ccaggtttag tgcaacgttg
     1261 catattggca atcagccctg ggatggtttt agcctcaagg aacatcagta ccacagtcga
     1321 aagaataaaa tcgtactgtc tatcaaatgt gaggttattc agatcaacga ctcgggtgtg
     1381 taaattatcc agattttcaa tggatttaat gcgctcgacg ttggcgatac tcatggcatt
     1441 tttatcccat gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt
     1501 gccacagccc agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagtacttc
     1561 agagtgtgtg cgggttaatt catatttatc agtaaaatag ttttcgtcac gaatgatcat
     1621 tctttgtcct ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc
     1681 atcagaagcg caaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac
     1741 agcggtaccg ccagcgtatg gaaaaatcca ttttcgctgc cactccccag atgcaaaccg
     1801 gtggttgcca gtgcagatac gccgaacgag aaactccaga atgaagcatt aaatggctgg
     1861 gagagatacc atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccaaaa
     1921 agcattttcg ccagcgtatc accctcgccg ccgttgacgc tcaaccaggc actacaggcc
     1981 accagcgcag gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc
     2041 gaactgcgca gacgctgcaa gatcacaggt tcaaggctta gccatgagaa aacgcctgcg
     2101 cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata
     2161 aagttgttgg caactgtcgg cagatacagt ccaggcgtgg tagcttcttc agggtgagat
     2221 ccgcgccata atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa
     2281 ctgaacaggc acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc
     2341 gtcgttgccg gaaacaaact cacaaaactg ctcagcactg gatggcgcac ttctgccagc
     2401 acgctatgcg gaaagcgtat aagtcgggta ataaatgcgc tagttaacaa tccccagatg
     2461 atcatcgcca gaatcaccag cccatcccct aaccagtggc tgacctgcca aacctggctg
     2521 gcatagcgcc aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc
     2581 ggcaaattga gcactttatc gctctgcatc ttcttactca ttttgtttaa tttataaagt
     2641 atatttgaaa tgcattttat ggaattttct cgagcatagc cagagccgca gaatttgcta
     2701 cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac
     2761 agcgagcctt tagttatcag gaagatggca ctaaaaaaaa tgtgttatta cggcagatta
     2821 tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg
     2881 aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaacgct attgcgttcg
     2941 gcggagcggt gatttccggt aatacgcgca tcaccggcac cagcgtgctg tggggagagg
     3001 tttacgcaac ggataatgtc tggatcgata atagtgaaat cagccagggt gcatatataa
     3061 gtgacagcgt caccattcat gactcactgg tttgcgggca atgtagaata tttggtcacg
     3121 ccttaattaa tcaacattct atgatcgtcg cagcacaagg cttaacgcct gaccatcaac
     3181 tcctgctaca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac
     3241 aaatttatgg cgatgccgtc atccggtatg cttttattga acatcgtgcc gaagtttttg
     3301 attttgccag tattgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgctaaag
     3361 tctacggtca cgcgcaggtg aaagctggca tagaagaaga tgccattcca acgattcatt
     3421 acagttcgca ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg
     3481 tcttgattgg cggaaatgcc gtggtacgtg gtggaccgat tctgctcgac gagcacgttg
     3541 taatacaagg tgagagccgt attacaggtg cggtgataat tgaaaaccat gtggagttaa
     3601 ccgaccacgc cctcgttgaa gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag
     3661 tcattaacgg cgaggggcgc attacgcgaa cgccgctggc agggttatta tgaatcgata
     3721 atacgcgcgt ataagttcac atcatcatag gcatcattca ggaactcagc ctgtttcagg
     3781 caaccttcaa ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg attgtccacc
     3841 cgacatttga tcacgaagcg tctaagttca ccagactggg cgtaatgatg aatcaatgcc
     3901 tgcagcgcct gagaaatgat cccctgcccc tgatgagatt cgtccagcca gtagcctatt
     3961 tcaacggttt tattcagtgg ttcaatacga ttaaacgaga taacgccgat aagttcatct
     4021 tctttgaaaa tcatgaacat tttggcatag ccgcgttgat gcaacatcac attaccctgc
     4081 accgtttttc gcgtgtcctc ttcactctga acgaactgcg gccagtttag cgactgctgt
     4141 aaccaggttt tatttttaca gattaactga taaagaggag tgacgtgatt ttctgcaaca
     4201 gcatgtaatt caagtgattc gcttactttt atcgtttcag tcatcttatc tccagtcaat
     4261 ccatgcagtt gttaaaaaag ccgcctggct ggcggcttag gaaagtttat acctctttga
     4321 cgctctcctg ctgcaggatt ttgagcatct catcttttaa ctgtagcttt tgttttttca
     4381 tgcggaccac ttccgcattg taccctcgac cgtcggaacc ttcctttctg gcaatttcat
     4441 gatcaagttt attgtgttta tcgaacaagg acataaagcg aggattttcg tttttcagac
     4501 gggatattaa atctcgatat tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt
     4561 gtatctgaaa tacacaccat cagattactc atttaactta gggaaaaatg cgagcgggat
     4621 cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga ttaactcatc
     4681 acgcggtcgt caacatactg acgtttatcc ggcgctggcg ggaaatactg atacagccat
     4741 gtttcactga tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac cggatcagtg
     4801 gagtccgaag tcggatacca gtcaaactga atacgataac catcaatggg ttcaatatag
     4861 agaatttcga tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc aatgcctttt
     4921 ggtgcggcag ctttcagatc accaccaacg aaatcgacgg caaaacgacg cgcccatttt
     4981 tcgggatagt gttcacctgg tgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc
     5041 gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg cactccagta cagacgatac
     5101 tggaatgcaa actcatcacc cgcctttaca gctttttctg gctgccagaa gcagacaatg
     5161 ttatccagcg tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc cttaccccac
     5221 ttgttacgcg gttccaccca cagacttggg cgtttgttat accagcccat aatgtcctga
     5281 taatgggaga aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg
     5341 taagcattga actgcaattt ttgcggatta ttcagcggac ggcaaatcca ctcgccgttg
     5401 ccccgccaca tggacagacg atcagagtca tgaatttgcg gatgaatggt gtcgcacatc
     5461 cgacgttcat tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc cagctgttta
     5521 atgtctttgc gcgcatacag gtgattttcc acatccataa tcacctgact tttctcacaa
     5581 tggatagtga acttataggc accagtaatg ctggcgctat cgagcaacgc ataaacggta
     5641 aaggtagttg cccccggttt taccgtatca aaccagaagg cggtaaagtc ggggaactct
     5701 tctttactgt cggtgtaagt gtcaatcgcc aggccgcggg ccgacaaacc gtattgatat
     5761 gtgtcatcaa cggcgcggaa ataactcgct ccgaggaatg atactacatc acggcgcgcc
     5821 agttcggggg ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg cccttctaat
     5881 tgtttggtat caacacctgc atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca
     5941 cgcgccagat gtgttgctgg atctacagaa aacatacgaa cgcggcgacg gaatcccatt
     6001 cccatatgga agaactgagc gtccagttga cggttctcaa cgttatgcca gagcgatttt
     6061 tcggcgtcgt attgaatact gttataagcc tgcggcgtca ttgtcgccag cgtgtcaggt
     6121 aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga
     6181 atggagaagt caaaacgctg ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc
     6241 gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca cggcggccat agccattgaa
     6301 cctttaataa atcgtctacg atccatacct gaaagtgagt ccttctggtg ttgtgaatgg
     6361 tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt tatgaccgct cactctagac
     6421 aatatttatt aataatccaa ttgttgaact cagagaatat gcataaatcg gcaatttttt
     6481 tatgtcgatg ggatgagcct gattcgtttg taaaatcagg caaatttttg caaaggcgga
     6541 tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct tcctgataaa
     6601 gctggaactc atccaaaacc ggacaaccca acgcctgctc aaatgcctcg cgactggcat
     6661 taccgtgatt gcgtgcctgc gtttcattac ccagatttga ctggaagatc cccgccgcgc
     6721 taacgggcaa gaaatcttca taggtaatgg gttgcgccgc gacccaacca cgttcaatta
     6781 agggctgtgg atcgtctccg ggatgaatcg cctgacgatg cgcctcacct gaaggcgtca
     6841 gacggtaccg gaaccatgcc agcccttgct gacgcattaa aaactcactg tcaggaaaag
     6901 tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg gttccagcgt
     6961 tacgcagaag atcatcatac agttgtcgcc ctttcggcgt taatgccacg ccacgctgct
     7021 caatttcacc aaagcgcgcg gtatgcgtgc cctgtttctg ccccgcaaac aacaccgtct
     7081 cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc
     7141 cctcgatgag aattttgggt tcaattccgc attcaggcat catcgactgc acccggtcaa
     7201 tatccagcgt acgtggcgtc aggtggttga tatggcatcc aggaaaacag accacatcag
     7261 caatcaaccg atgttcgttg tgcaaggcgc gataggtttc ttcatctacc gttgctgact
     7321 ggtgccagcg aaacgtttcc agggcttcct gcacaaactc ctgtgcctgt gtttcgttaa
     7381 aaccgccctg ctgctcatat tcctctaaca gttgtcgaca acgtggggtg aagatatcgc
     7441 gctgacgcag aatctccgcc gctttctggc gcaaaatttc gttctcgata agctcaaggc
     7501 ggagtaagga ggtaaaaacg cggaagggat tacgcgccag agaagcatca tcaatgggcc
     7561 gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag atcgtaatag ctcaccgggt
     7621 acatccccat aatggcaaac atccgccgaa gagtagcaag ctcttgtgca gtcccaacgc
     7681 gaatcgcccc atgacgttca acattcagtc gcgccagctc gtctgcattt accatttttt
     7741 cgtgcagttg aggattgttt tccagcacag ccagattcac atcagctacc agttccagca
     7801 gcgtgccata ttgcggaact tcttgctggt acatggctga cattgcctgc gaaaactgtt
     7861 cccgaatctc atccgccgtg atgctgttcg ccatagtgtc tgcctccagt ggtcagtaat
     7921 ctggagtgta ggtaaccgca ttcactctgg cgggaagaat ttacaaactg tgatctcgcc
     7981 gcgaaaacat caatattatc cattttgctg taacataatt gctttaattg ttaataatat
     8041 tttgcaatca agttatcata atcaaacaac ttcacttgtc agcgacaccg cttcgttttt
     8101 aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc gccaccttca
     8161 tacattcgta gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa cccttaattt
     8221 gagtcaacct gcgctctcta agacattgaa tgaactggag cagctgactg gcgctcgctt
     8281 gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat ttttaacgca
     8341 tgcagtcaga gttcttgacg ccatcaacac tgccggacag tcgcttcatc gtaaagaaga
     8401 tcttaataat gatgtcgtca gggttggtgc actacctact gcggcactgg ggatattacc
     8461 ttcggttata ggtcagtttc atcagcaaca aaaagagacg accttgcaag ttgcgacaat
     8521 gagtaaccct atgattctgg cgggtttaaa aaccggggaa atcgatatcg gcattggtcg
     8581 gatgtcagat cctgaactga tgaccgggct taattacgaa ctgctgtttc ttgaatcgct
     8641 gaagctggtt gtccgcccta atcacccgct acttcaggag aacgtaacgc taagccgggt
     8701 gctggaatgg ccggtcgttg tatcaccaga aggcactgcg ccacgccagc attcagatgc
     8761 attagtgcag agccagggct gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc
     8821 gctatctcgt caacttacgg ttgaatacga ctacgtgtgg tttgtccctt ctggcgcggt
     8881 aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct gttccgggac atggtgcagg
     8941 cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc tcttctggtt gccagttgat
     9001 gattaacgct attcgaaaat caatgccgtt ctgaaaggtg aagggatctg tcgatccctc
     9061 cttgaacatt ttcacaccgt agcgaaacta actggttcac ccgctccgcg aggttctgcc
     9121 gacacagaat gtttgttcag acggaatacg tccaccgcct cagttaatcg tgccgcctgt
     9181 tcttcaaggg acaccgccgc tgctgaggcc tcttctacca gagaagcatt ctgttgcgtc
     9241 accttatcca tttcagaaat cgcctggcta acctgcgtta tgcctctact ttgttcatcc
     9301 gaggcggcgg cgatttcctg catgatatgt gtgacactcg cgacggcatc aacaatagtg
     9361 ctcatggttt tccctgccgt tgccacctca tccgacccca ggtcaattaa cctgactgat
     9421 tcactgatca agccttcaat ctctttcgcc gcttgggcgc tgcggcttgc gagtgtccgt
     9481 acttcgctgg cgacaacggc aaatccacgg ccttgctcac ccgctcgcgc ggcttcaacg
     9541 gcagcattca gtgccagaat attcgtctgg aaagcaatac tgttgatgac ggcggtgatc
     9601 tcagaaattt tcttcgaact cgtggagata gcgcccatcg tttttactac accggaaacc
     9661 gtctgcccgc catcgctggc tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc
     9721 gcgttatccg cattctgttt caccgtcgca gtgagttgct ccatgctggc ggcagtttgt
     9781 tcgattgccg ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt
     9841 tcgctggtgc cacgataaat ctcttccgca ccctgtcgaa cagtccctac tgtcatcccc
     9901 agtgaatgct gcatttgctg taaatgacga tttaagcgac cgatttcatt acgacccgcc
     9961 ggttcatcct tcatcgtcag atcgccacta gcgatttttt caatccgttg tgcggcatgt
    10021 tgcagtggac gaatgacgat ccgacgtagc accataaatg ttatcagcgt catgaccagg
    10081 gcaagcacaa acgcgccaat catgaacatc ccacccaggc gggtgcgctg atgggccagt
    10141 tccgccagtt ggttggccct ggtgctacgt atcttaacgg ctttgttcaa aatatcggta
    10201 taagcattat ccagcggtcg gatctgctca ctttcatgat taataatcgc ttcaaacatg
    10261 ccatttttgg catatttcaa cataggttgc atacccgtga tataagcctg aaagcgttga
    10321 tttaattcag tgtcgagggc ttcatcagca ggtgttttca ccggtcgatt ctgataagca
    10381 cgataacctt gctgcgactg tttaatctcc gattcggctt gcgcaatatt gcgcttcatt
    10441 gcttccattt ccgcaatacg actcgcagcc ccggcctgaa tcatattgat ccgggctgaa
    10501 cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct caatctccgc agtaacatca
    10561 cgctgatcgc gatcggcctg ccagaggaaa tagccagcca gagcagaact taatgcgaat
    10621 agaactaaga taccgccaag aatgcaggca aataacggaa ccagcctgat gtgatgcaaa
    10681 aaacctaatc gatgtgaggg agttgtattc attaccgttg tctctcgtcc aggtttactc
    10741 ctgatagaaa gtcatcggca cttgacggta attacttatg accaaaaacc tctttgcgtc
    10801 acatttttac aacaattatt gcagagaaaa ttaatgctat tggaatgaag agagtaacga
    10861 atggctaaac tggctaaccc gaatgacgaa aaattctgga aaacagcacg caatttcgtg
    10921 aagtgattat tttcgtccct aaggaaacct cataggatgc ctgcgggtgc ctgagacttt
    10981 ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac
    11041 cgaggccaac gaatgaacct agcaattcag atcttagcct cttacccgcc atccggcaag
    11101 gagaaaggct atgaagcaca accctctggt ggtgtgtctg ctcattatct gcattacgat
    11161 tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc gggacggtga
    11221 taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc ggggattttc
    11281 cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac ttcagttagt
    11341 gcatgttttt ttcgaggcac taatagaacc atcattacag acaaatttgc cgcctgccgt
    11401 acagtgtgag atcccccctt tcgaaccaga acagggtttg cgcccggcat cggcataaaa
    11461 ggaaacaagc gttcccataa acaaaataag tgccagtttt ttcatataac ctccatggtt
    11521 tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata ccgttgatga
    11581 gatcaaagat cgggtgaatt gattaaaaat aagccagatg gacgtatggg caaggattat
    11641 ggttttattt gtcatacaaa taaatataat aggcgcttcc gatgtagacc cgtattcttc
    11701 gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt caggaacgtt
    11761 tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt gccagtgcag
    11821 ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcgtctca tgccacgact
    11881 ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc aaaccaaacg
    11941 caaaccacgg gttgccccgg ttatacatca tcaccaaacc aatcactggc agcgcaataa
    12001 acaggagata aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc ggttttggct
    12061 taggtataat cggcggggtt gggtatttca gtcttaacaa aagacgaacc accatcagca
    12121 cgagaattga gataccacag gaaacatgaa ccatgttgat gagtggccga tcgctacgtg
    12181 ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa aagaccagcc
    12241 agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct gctttaacat
    12301 aattttccgt taacataacg ggcttttctc aaaatttcat taaatattgt tcacccgttt
    12361 tcagtacgtt acgtttcact gtaagaatgg agattgccca tcccacaatc ctgggctaag
    12421 cctggaaagg atcaattttc atccgaacgt tcctgacagg agaaaaccat gagtaaagtt
    12481 ggtattaacg gttttggtcg tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa
    12541 agcaacatag acgttgtcgc tattaatgat ctcacttccc caaaaattct cgcctatctg
    12601 ctgaaacatg attcaaacta tggtccgttc ccctggagcg ttgattttac ggaagattca
    12661 cttatcgttg atgggaaaag tatctcggtt tacgccgaaa aagaggctaa aaatattccg
    12721 tggaaagcga aaggggcaga aatcattgtc gaatgtactg gcttttatac ctccgccgag
    12781 aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt
    12841 gaaatgaaaa ctatcgttta taacgtcaat gacgacactc tggatggcaa cgacaccatt
    12901 gtttccgtgg cgtcatgcac cactaactgt cttgcgccga tggccaaagc cttgcatgac
    12961 agtttcggga tagaagtcgg cacgatgacg accattcatg cctatactgg cacccagtca
    13021 ctggtggatg gcccacgtgg taaagattta cgtgcttcac gcgcagcggc agaaaatatc
    13081 attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccgga actgagcggc
    13141 aaactgaaag gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac tgaactggta
    13201 tcgattctcg gaaaaaaagt gactgccgaa gaggtgaata acgcacttaa acaagcaacc
    13261 accaataacg agtcatttgg ttataccgat gaagaaatag tctcttccga tatcattggc
    13321 agccatttcg gttcggtgtt tgatgccacg caaacggaaa ttaccgccgt gggcgattta
    13381 caactggtga aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac gcagcttatt
    13441 cgcaccctcg aaaaattcgc taaactgtga cgcgcacagg cggaggaaaa aacctccgcc
    13501 tcttttactc attacgactg taaataaacc acctgggtct gcagatattc atgcaggcca
    13561 tgtttaccat ctgcgccgcc aataccggat ttacgccatc cggcgtggaa gccttgcata
    13621 gcttcgaagt tttcacggtt gatgtaagtt tcaccaaact tcagcccttt aatggctttc
    13681 atcgcgacgt tcagattttg ggtatagatt gatgaggtca ggccgtaatc actgtcatta
    13741 gccattgaga tagcctcttc cagcgtgtca aatgcgacca ccggcagaac cgggccaaag
    13801 gtttcctcat gcataatcga catttcctgg cgaacatcca gcagcaatgt cggcggataa
    13861 taatatcctt tcccctctac cgctttgcca cccaacgcca ctctcgcccc ttcttctact
    13921 gcgcgcgcca ctttttgctc aaccctttcc agcgccgcgg cgttaatcaa cggccccatc
    13981 gcaatgtcgt tgcgttcagc ggggttacca aattgaaccg cctgcatcgc ttcacccagc
    14041 cgattgacga actgatcata aatgcctttc tgtacataaa cacgttctgc acagttacac
    14101 acttgcccac tattaatgac gcgtgaatca acgatggctt tgactgccag ttcaagatcg
    14161 gcatcgtcca ttacgatagc tggtgcttta ccccccaatt ccagacacac tttggtgatg
    14221 tttttcgccg cagtcgccat gatcttctca cctgcagaga cgctgcctgt catactgacc
    14281 attgcgacct ttgggttacc cgccagttct tgcccaacgg tttcaccacg ccccagtaca
    14341 aggttaaaca cgccgcgcgg aaggcctatt tcatcgacga ttttggcgaa tgcaatcgca
    14401 ttgtttggcg taaattcact aggtttaatg acgatggtat taccggtcaa aagagcggga
    14461 gccattttgc gggcaatgag gaagaacggg aagttccacg gcagaatgcc ggtagtcaca
    14521 ccaagcgcac gtttaaacaa aagaatattt tctcctggac gatcgctttg aataatctcg
    14581 ccctcgtaac gccgtgccca ctccgccatg taatcgatat agtcggcagt aaaagccact
    14641 tcgacttcag ccagctgctg gatcttgccc ccttcttcaa caatcagcgc actgatttca
    14701 ctggcgcgtt cgcggatccc ggcggagatt ttgcgcaacc aactggcgcg ttcaatagca
    14761 ggcaacgctt cccattctgg ttgtgcacgt tctgctgcat cgattgcctt acgggcatcc
    14821 tcggcctgac catcgggtat gcgggaaatg acagcctctg tagcagggtt taccacatca
    14881 atccatgcgt ctccacgcca ggtaacaaac tgtccatcga tatacatagg atgttgaacg
    14941 ggtactgaca tgggcgactc ctgtgattta tatgtttgtt ttcggtgaat gcattgttaa
    15001 ttaacatcaa tcatcagaga gacggcatcg tgaaaggaca attctgtgaa gggcttcata
    15061 aaaacaagtg taaaaggaat aaaaggcaga aataaaagtc aggagtcatg cccgtttgca
    15121 aacaagtggt tcacaaacgg gcaattttta acgtaacgag cgactttcca tcgcctcgat
    15181 gagcaccgca tcatgtttca gcgtttgcca tgcatggatg acttctgccg gaaaatcaac
    15241 gtgaacgata aaatctcgcc cgcggggacc gtagccatcg ctatcatcgc gtaaacgcgg
    15301 cagctcgcca gtgagtagtg agagataacg ctcaactggc cataatcctt gtaactgatt
    15361 aataaagatt acactgtctg cgttatttcc taactgagga acgaatccgg gataacttaa
    15421 ccagcgtggt gcatcgggat tgtccccagt catacggcgg aacgtcgcca tcgtgcgccg
    15481 ctgcatggtg gggtcctgaa caacgatagc cgtatgaact cgttctacgg cctgattcaa
    15541 tagcgcgatg ctaaagcgtg cgttttcacc gcagtttgtt gactggtctt caatccagat
    15601 tttttcatgc ggaatgtgcc agaactgatg agcgatatcc gccaggatgg tcgcttctgc
    15661 tctgccagtg gtgcggatag tgttgtagtg cggatgctgt gcgatggcgc tatacaaaaa
    15721 agttgtcgag tgaccgatac caccactaat cagtaaagga atttgctgat cgcgagcaat
    15781 cttacatgcc gcgtcgatag tcggcataac cgcattgcct gcaaggatca cgcaatcggc
    15841 ctgatacggc acctcaccgg agaaatcatc ctgcgccagc cactgtccga taacatttat
    15901 ggcatctata gttgccggcg aaagcgtcgg aaacggggta atgttcatgg ttcctcctta
    15961 tcgttccctt aagaatactg cgtgagataa tagtgagaag cggagcactc tgaaaggagg
    16021 aagtcgtatt tccttaaccg ccactccgca agcctgacat atcttttaag gaaatgctgt
    16081 agataaacat tattcatctt gcagcagaga attgtatttt tctgaattcc tgaattttta
    16141 cccttaccgt tttagaatta ataaaaaacc cggcgctaaa tgcttaccgg gttttttctt
    16201 tatcaggcaa atagcagtgt taaccgctaa tctgctccat cgcctgcaaa atacgcttat
    16261 ccgaaatagg ataaggcgta ccaagttgtt gagcgaagta actaacgcgc aactcttcta
    16321 tcatccaacg gatctctttc acgtcttcat cctcacgacg tgcgggcggc agtttgttga
    16381 tccattgctg ccacgcctgc tggacgtttt cgactttcag catctgcgca cggtcgcgat
    16441 gtggatcaac cgccagtttt tccagccgtt tttcaatcgc ctgcaaatat cgcagcgtgt
    16501 cgcccagccg tttgaagccg ttaccagtga caaaaccgcg atataccaac ccgcccatct
    16561 gcgctttaat gtcagaaagc cccagcgcca tggtcatatc cacccgccct ttcagacgtt
    16621 tgttgatatt gaacactgcc gtaaggattt gctcgacctg cttcgcaata tccaccaccg
    16681 tgtcgttcag ttcggcacgc actttttcat gcagcgcagc aaagccttct tccgtccaga
    16741 ccgggccacc attggcgtcg atcaatttat ccacaccgca ggagatacag tcgtcgatca
    16801 gctccagcac tttgccatac gggttaaagt acagtcccag cttggctttg ttcggtaact
    16861 tttcatgtaa atatttgatt ggcgatggaa tattcagcag cagtagacgg cgaagaccgt
    16921 tccacattgc ctgcttttgc tccagcgggt tatcaaacag tttgatcgcc acactgtcgc
    16981 gctcatccac cagcgccggc cacgccttca ctttgtagtt gccacgcttc tgttcgtagc
    17041 tttccggcag ctgaccaaaa ctccagatat gtaagccgct ctgctcgata ccgtcatccg
    17101 ccaccgcgga cagcgtttcc tgcactttgc ctttcagcgc atctttcaga tcctgtaatg
    17161 agcgcccttc ttttagcttc ttatttttgt cgtccaccac gcggaaggtg attttcaggt
    17221 gatcgggcac ctgatcccag tgccagtctt cgcggtcaac ggtaacgccg gtcatccgcc
    17281 gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag cggtttgacg cggcctaaaa
    17341 acgcttcggc atagtttggc gcgggtacaa aattacggcg taccggtttc ggcaacgatt
    17401 taatcagagc aatcaccagt tcgcggcgca gaccggggat ctgccattca aacccgcttt
    17461 cctcaacctg gttaagtaac ggcagcggaa tatgtacggt cacaccgtca gcatccgcgc
    17521 cgggctcaaa ctgatagctc aaacgcagct tgagattgcc ctgatgccag aagttcgggt
    17581 aatccagctt gctgattttt tctgcgccct ctttgatcaa catgcttttt tcaaagttga
    17641 gcaaatcagg cgtttcgcgg ctgacttttt tccaccagct atcgaagtgg cgcgcagaga
    17701 tcacatcgtg gctgatgcgc tggtcgtaga actcaaacaa cgtttcgtca tcaaccagaa
    17761 tatcgcggcg acgtgatttg tgttccagct cttccacttc ggcccgcagt ttcaggtttt
    17821 cacggaagaa tgcgtgacgc gtctgccagt caccttccac cagcgcatgg cgaataaaga
    17881 gttcacgaca taacgccgga tcgatctggc tgtagttgac cttgcgcgcg gcaacaatcg
    17941 gcaaaccata aacagtgact ttttccgttg ccatcaccgc gccctgcgcc cgttcccagt
    18001 gcggttcgct gtaggtacgt ttaatcaaat gctgagcgac aggctccacc cattccgggt
    18061 cgatacgcgc agcaatgcgc ccccacaggc ggctggtttc taccagttcc gccaccatta
    18121 cccatttcgg cggttttttg aataaaccag aaccggggaa gatggagaaa cgcgcgttac
    18181 gtgcgccggt atattcttgt ttatcggcat ctttcatgcc gatatgggaa agtaaacccg
    18241 tcagcaacgc aatgtgaatt tcgcgatact ccgccggttc gctgttaacc ggaatgccaa
    18301 gttctttcac cacctgacgc aactgggtgt agatatcctg ccattcgcgc acgcgcagat
    18361 agttgaggta atcggtacga cacaggcgac ggaaggcgtt ggaagaaagc gccttttgct
    18421 gctcgccaag ataattccac agatttacaa acgcgaggaa gtcggattct ttgtcgtgga
    18481 agcgacgatg tttttcgtcc gatgcctgct gtttgtccat cggccgctca cgcgggtcct
    18541 gaatggagag cgcggacgtg ataatcatcg cctcacgcac gcagccatgt ttctgcgctt
    18601 ccagcaccat acgcgccaga cgtgggtcga caggcaactg cgagagctgg cgaccgagcg
    18661 gcgtcagttt ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt tcttcgagca
    18721 gacgcacgcc atcctggata ttgcgtttat ccggtgcttc gacaaacggg aacgcagcga
    18781 tatcgcccag ccccagcgcg gtcatctgca aaataaccga ggccaggttg gtacgcagaa
    18841 tctccggatc ggtaaactcc gggcgcgaaa ggaaatcgtc ttcggaatag agacgaatac
    18901 agatcccttc ggacacacgc ccacagcggc ctttacgctg attggcagac gcctgcgaaa
    18961 tgggttcaat cggcaaacgc tgcactttgg tgcgatagct gtagcggctg atacgtgctg
    19021 taccggggtc gataacgtat ttaatccccg gtacggtcag cgaggtttcc gcgacgttgg
    19081 tcgccagcac aatgcgccgt ccgctgtgcg actggaacac gcggttctgt tcgctgttcg
    19141 aaagccgcgc ataaagcggc aagatttcgg tatgacgcag atttagcttg ttcagcgcat
    19201 cggcggtatc gcgaatttcc cgctcaccac tcataaagat cagaatgtcg ccagggcttt
    19261 cctgactcag ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc tcggtgtcgt
    19321 cggcttcttc aacaatcggt cgatagcgca cttccaccgg ataggtccga ccggagactt
    19381 cgataatcgg cgcattatta aagtggcgcg aaaagcgttc cgggtcgata gtcgcggaag
    19441 tgataatgat ttttaggtca ggacgccgcg gcagcaactc tttcaaatag ccgagcagaa
    19501 aatcgatatt caggctgcgt tcgtgtgctt cgtcaataat gatggtgtcg tactgcatca
    19561 gcaggcggtc ttgctggatc tccgccagca ggataccgtc ggtcatcagc ttgaccatcg
    19621 tgttatcact aacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg cccggctccg
    19681 ttttcagctc ttccgcaata cggttcgcca ccgttcttgc cgccaggcga cgcggctggg
    19741 tatggccgat cagcccttta atcccgcgcc ccagctccat acagattttc ggtaactgag
    19801 tcgttttacc agaacccgtt tccccggcga cgatcaccac ctggtgatca cgaatcgctt
    19861 cgagaatgtc ctgttttttc tgactaacag gtaaattgtc aggataagta atttccggtc
    19921 gtgccgcttc acgcagcagg actttccctg ccgcttggtc aatctctttc gccatctcct
    19981 ggaaaatggc ctgttgtgca tcaggatttt taaccttctt aacaccgtgc agacgccgag
    20041 aaaaacgcag tctgtcgcgc agcatcagcg aatccagccg ctgctgcaag gccgtaaagg
    20101 tcaatttttg ttgttctgtc ataacgttag tgggcagtag tctgcctgat taattttctt
    20161 ttaaatgata gatatagagt accacatcgc ggctttctat gcgttgttca ataaattcga
    20221 acataggctt cgatttattg cgctatctct gtggcaggat tgtgaataaa gtgtcagcaa
    20281 gcaacggggc atcgcccatt caaacatcta taaggaaaca ccatgagcaa ggtattagtt
    20341 cttaaatcca gcatcctggc agggtactct cagtctaatc agttgtccga ttattttgtt
    20401 gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct ggctgcaaat
    20461 ccgattccgg tactggatgg cgaactggtt ggtgcactgc gtccgagcga tgcgccgctg
    20521 actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga gctgaaagcc
    20581 cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac tcagttgaaa
    20641 aactattttg acctggttgc acgcgcaggc gttactttcc gctataccga gaacggtccg
    20701 gaaggtctgg taacgggtaa aaaagccatc gttattacca gccgcggtgg gatccacaaa
    20761 gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt tatcggcatt
    20821 accgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat ggcagcgaaa
    20881 gcgcagtctg acgcgaaagc ggccatcgac agcattgttg ctgcataacc ggacacgatc
    20941 ttgatgtgga ctgtagttca gtccacatct caatccactt acctgttttc ccataatttc
    21001 agcatcgctt tgtgatcttc cttaagcaca atatgcgaac gtctggctcg aatataacta
    21061 atcgcttcat caacggtttt acagtgtccg taacataaca accatgccgc cactaccagc
    21121 gcactgcgcg ataatcccaa cgcgcaatgg accagaacgc tgccttgctc ttcgcgtaat
    21181 gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac cagatccagc
    21241 atcggtacac agaaatagag tcgatctttt gtcgctcgtc cccgagggaa ttcaaaggtg
    21301 acgtccagaa ccgcattctg tgccggaata tgtcgtggaa acgcccctaa ataaacacca
    21361 gcagtaattt tgctcaccgg ctccaggcga cgacaaaacc aacgcataga cagccacatc
    21421 ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc ctgactatct
    21481 ttccctgttg ttatcgcgcc aagcccaccg tacccacggc caataatgag tagcgataat
    21541 actggccaac ataaccagac tgaccaccat aactgaatca tcaccattag ctccatcaac
    21601 acaatgcacg agcccgcgcc tacgacatat ggcagtgcta ttttgattcg acgttgatca
    21661 ggtttctgat aattccaacg acgatcgacg ggcaccatcc agtcaatcaa catacctacc
    21721 gccagccctg tgatgacatc aataaaatga tgctgccagg tcgtcagagt agaaatagcg
    21781 atgagtaaaa accatccgcc acagacttta cgccacctct cagccagatg ctgacgaaag
    21841 tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt ataaggcaga
    21901 tcaaacagtt caagttgcga aaatagccat cccgtcaccc cactcacttc aggacggata
    21961 aaactaaact tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt tgccagaata
    22021 agccggtgga caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag cgaaaatcca
    22081 tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt
    22141 tcccaaccga atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac
    22201 tgattaagag atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg
    22261 ccttgtagca atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa
    22321 aataccccac tcatcaatcc gttgtgtgca tttatcaaat ccggcatcac gcacgagtga
    22381 atccatctcc ccttgcgaac gcacgcgcat tacccacggt ttaccatctt tatgactggt
    22441 taacaccccg gcaatcatct ccagttgagg gtgccacggc tgcccggtgt agatgagaat
    22501 gccgcccggt tcgatggcat tggcaagacc tgcgagtgag ttttttacct gctcattttc
    22561 gggaaaaagc tcatacaggc cagagacaat cgccagcgta ggccgcggag ttaacgcgct
    22621 gagttcctcc gggttaaacg catcgccctg ttcaaaacgc acccgcccag acattccccg
    22681 ttgggcaatc atcgcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat
    22741 atcgcttacg gcaggctcgt ttgccagcgc atccagtaca tagcgcccat gccctgcggc
    22801 aatgtcaacc acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat
    22861 cagtatttgt aaatgggttt tacgctggcg aataccgcgc cagccaacac tgttcaggta
    22921 gattttgtca attaagcgcc cgaatgcgtt actgccttgc ggttgatttt gatagacata
    22981 atccagggaa ctgcctgaat caaagccggt gctcattccg agatgcaggc ccgcagagtg
    23041 cgccccgaac aatttcatcg cctttcgcat aaagcgatac gccaaatcaa ccggcgataa
    23101 tggcacgggt ccacctgaaa ggagccgcca gcgatccgct gatggtccag tgcggtcttc
    23161 atgctgataa tcaaattttt gtgacttgtt agcatataaa cgactaataa agctttgcat
    23221 tttttcaaat gcctgcgccc tgttctcttc acccaacgtg tcgtgataaa agcctggcag
    23281 cagatgcagc tctttcagag ggctacgtaa tctctgataa aaatcaatct gcggttggcg
    23341 atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc
    23401 atctctaata atacgttcag acgttttgta gagatcgagc aagatattaa cggcaatcgc
    23461 ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata
    23521 gcgtcctttc acataggaat taataaaaaa cagaccacgc agacgatgcc ataacgccag
    23581 cgcaggacgt gccagcggta catacaattt aaccttaaag gccggagaag ccagcaccag
    23641 cccgcgaatt gcaggcgcat aatcatgaac ccatgtggca accagcactg cgccgacgct
    23701 ttgcgcgatc actaccacct catccagtcc gacctggcta tcgctggcag caaaacgaac
    23761 aaattcatcg acatcctgca ctgaacgcgc aagagatggg ctataaccac gcggccccga
    23821 agtttgtcca tgccctcggg catcccaggc ataaaaaaca gtatctggca tcgccagttc
    23881 atcaacgaga tgttgtagac gaccagaatg ttcatgaccg cgatgaaata agacgatgac
    23941 ctttttagcc ccgggctgta aagcgggcca gtgccgataa aacaacgctg tattatcact
    24001 gctggtaaaa aaatgttccc catggatgcg tgaattttcc attttctcta ttttccttca
    24061 gtaacagcag tagcgaataa aataaaaaaa taccggagcg gtgaaaatta atgagtcaat
    24121 cctgtcaagg agtccgccat gtcctggtaa tagtttccca ctatctttaa caccaatatc
    24181 tcgtttgatg gctgacatca cgacatcgcc gcaaaaacca ctaataccaa ttaacaaacc
    24241 cgctaataat gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa tcagtgacgc
    24301 aatcatggtg gtgattacgc cccccaccag accttccagc gtttttcccg ggctgacttt
    24361 ggggaccact tttcttctgc cgcaggattt cccccataaa tactgtgcaa tatcgtttga
    24421 ctcggttaat gccaaaagaa ataacaccag taacgccccg ccctggatat ttgtggttgg
    24481 caacattaat aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc agccccaaaa
    24541 aatggcgctc acggtatgca gaaaaccgga ggggtccccc ataaaaactt gcccggtggc
    24601 taatattaga aacccagcca aaggtataaa taatagatac agctcaaagc aattaaaacc
    24661 gataaacaaa tagttaagag gaataaccca atataaccac cgcggaaaat gtacagatat
    24721 aagcgtacaa tattctttca atgccagaaa acttatcagc gcaaaaaacg tcaacgtcat
    24781 ccagcgtgga cctgaaatca ccagtgaaaa acaaatgacg atgccccacc aggtacgaat
    24841 acgtaatgta acttcatcct cttttctctc cggtaaccgc catagcagaa aatggtttat
    24901 cagcgttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca gcattatctt
    24961 tcagcgctca taagaagcac actccgacaa cgattaatcg cagtccagag aagcagaatt
    25021 gaagcaatgc tccataaaag attattccac tgcatccact gtggataaat ggcaacggcc
    25081 agaccccaca gaccaaatat taacgcgcga tcgcttttgc caaatggacc ggcataactg
    25141 cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat ggtgcaaaat
    25201 aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaatgg taaatagagc
    25261 gcaatatcgg aaataacatc accagtctca ttcaaaattg ccccaagccg tgtttgctga
    25321 ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat aaaaagcacg
    25381 atgggcaata gcataaagag gatgggttgt gccaccaaca tcaacagcaa tccggtaaga
    25441 agagaaagcg ccagtgcagc aagagtgatg tgattcgctg taacgtggtg cttgtaaagc
    25501 caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta gagcgtcatt
    25561 atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttcccctgac ggagatatcg
    25621 caacacgccg cttcattaaa aattcagaat atatcaaggt attggggact cactgtcagg
    25681 aataattata cccaattaat caaagggtta agaaatatta cgttgtggat caatacaact
    25741 catcaagttt tctcatcgct tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct
    25801 ggcggactaa atccagttca gggttaacaa gctctaacgc cagcaaagga tcacgtttca
    25861 gcctcgcctt taatacatca actggaaaac ggggattatt catcgccaca atccaccact
    25921 cgggatcaat ttctgccgcc agtactgcag gcggtgtgtg gcggttgtta agtgcgccga
    25981 acttgaaaaa tgtgagattg cttttcgcca gtgtactcca ccatatacga tccggttgtt
    26041 ttgccagtac atccagttgt gctaacgcat cggggtcgag tgcataatag acgcgtaatg
    26101 cttcatcaac ctgcgcacgg gagctgtttt cctgcctcag aaaaaacttc gcccaaatat
    26161 cattgcgtgg cgtattttgc tgtaattcct tgaggacaac agcgggtaaa ttatcccaac
    26221 taactaatgc actctctaaa aagtcccagt cttccaaacg tatcagggcg gtggcattta
    26281 atagcatctg atcatcaact tcaccgtggc tgataagctc agacaaaatc atatcagcgc
    26341 tggtttcata cgcagctgaa tctgctttcg gattattttt ccgatgcgat aaagcacgtt
    26401 gccaaatttt ttgtttcatc gacgtgctca gatactgatt tttcgccaga ttactccacc
    26461 cctcaggatc gttgaaacgt gtcaataacc gttgtagagc agcgtcagag tgaattacct
    26521 gcatatcccg acagcaaaag taggcagggg attttttctc catcctttta acaaaattaa
    26581 actgtaagtt atctggcaat gctaaaaata acacacgtat tagatcatcg tcattctcga
    26641 atttttggta taaataggct gcaactttgt cactctcttg tggcgtcatc cgcaataatt
    26701 gaccatggtg tttcatctct gccagcttga acgctaagct tgtgacaaca ttgtttgatg
    26761 aatcgttcgc caactgcatt gcctgattgt aggtgagttg tcctccagca ccgacctgac
    26821 gaatagagga atcatcactg cggatatagc catcaataat ggattcaggc aataaccacg
    26881 catctttata aagctcctga atgatcatat cccaatctct tttcgccaga gtcattacga
    26941 tatccagagg gagattttca cgtagcgcaa gattcaacgg accgacacca tcaggtagac
    27001 gactcattcg cgtcattatt tcgacagaga gcggttcacg gtcgagcaac cccagaacaa
    27061 caggctggta acccaattgc tgatcggtca actccagaat ctcattgtca ggcatatatc
    27121 tcgccatact ttcaatcgtt tgttcatcat tgctattaat aagttgtttg cgtaggttag
    27181 ctgaaattcc tttaagcctt gggagagttc gcattatggc aagatcaaat gtcgcaatat
    27241 ctcgtccttc aaatgcagca acgttgcctt catccagcat ttgcaattcc tgcactaact
    27301 caggagaagg cttagggcga aacagcgctt cttttttcat ctgattacag acattccctg
    27361 ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc atcttcatca tttattagac
    27421 cccaataata tgcatcagga tttattatca tcagcgcttt tttgacattt tcagtgtcat
    27481 ttttgtcact cgcacggacc atgaattcac tatttgtcga agacgcaaca acaggtgcaa
    27541 ttatgtcatt gataaatatt ccacgcgcat taatactccc cttaatcgaa gacggattat
    27601 cgtaatatga gtcgataaat accagtccac ttgcatttat cgaaccggta attaacagtg
    27661 gtgaatccgt aactatattc gttgccgtca cgttaccgag aataacccat ggattagaaa
    27721 gagagaaagt ggaaatatta cctttgactg tcaaattacc aagaacaatc aatttttgat
    27781 tactctctat attaccttct accactaaat cgccacgatg cactttccag tccgatgcag
    27841 ataaaccgaa ggggacaaga ttcacgctac tgttgatagc ttgttcaact tgttgtttat
    27901 cttcagcggt agccgcagaa aaaaatggtt caatatcaac tgtatttgca acacatcgaa
    27961 gttccgcctg tactggaaga ctcagcataa acaggggaag taaacctatc cagccgcaaa
    28021 tcctggcctt aaacatatgc actccatgcc attaaaaaag aaacaaaagg gaaatgcatc
    28081 tttccctttt gtatattcgt tattctcgcg aaataccatc caacgtagac aacacttcct
    28141 cagaaagatg caatttttca gcagccatat tctcccgtaa atgcgcaaca gaagacgtcc
    28201 ctgggatcag caaaatattc ggtgaacgtt gtaacagcca cgccagcgcc acctgcatgg
    28261 gtgttgcacc caggctcgca gcaacatccg acagcgtaga ggattgcagc ggtgtaaagc
    28321 ccccgagcgg gaagaacggc acgtaggcaa tgccgtcacg agccaaagca tcaatcattg
    28381 cgtcatcagc acgatgagca atgttgtatt cgttctgcac acagacgatt tcggcaatct
    28441 tgcgcgcctc tgcaacctgc gtcggtgtga cgttgctcag gccaatatgt tttaccaggc
    28501 cttgttgttg catctctgcc agcacggtca ggctggcttc aatcgatcct tccgctgggc
    28561 catgaccatc ccccataata acgcgcaggt taaccacatc cagtacgtcc aggccgagat
    28621 tacgtagatt atcgtgcacc gctttttgca actctgccgg agaaaaggcg ggcaaccagg
    28681 aagcgtcctc tccacgccgc gcaccaattt tagtgacaat tgtcaggtcg tcagagtaag
    28741 gataaagcgc ttcgcggata atctgattgg tgacgtgcgg accataaaag tcgctggtat
    28801 caatgtgatt gacgcccaat gccagcgcct cgcgcagcac ggttatagcg acgtggcgat
    28861 ctcttggggg gccaaaaacc ccaggacctg ccagttgcat cgcgccataa ccaagacggt
    28921 taacggattt tgtaccgaga gtaaatgtat tgctgctcat cgcaccctgc cctgtaataa
    28981 aacggattaa gaaaaatcag tatagaaacg aaaagagata aataatgcga gggcgtaaac
    29041 aagattactg gatgagttgc tctattgtcc tgaaaaacac aaaacaaaac gcgtaaagcg
    29101 ccgcatccgg caataaaaaa accgcaacct gtatcagcgt tgcggtttag gtatcacatg
    29161 ctaaaaatta gaatgttcag tatctggacg ttactgtttc ggtcgcagat tctcatcaaa
    29221 taccagacgt ttcctgttcg gttcaacttc ccgcaatggc tcaacttgat gcaacgtctg
    29281 cttacaacga gtcaccagca cctgatactc atgcgtaccc tgctttttcc atgccagctc
    29341 ctgctcactg agttcacgaa tcgctttcgc cgggctgccg acaatcaggt aattggcagg
    29401 catttctgct ttggctttca caaatgcgga tgcgccaaca atgctgttct cgccaatcac
    29461 cgcgccgtcc atcactaccg cgttcattcc cactaatgca ttgcggcgga taatgcaacc
    29521 atgaaggata gcgctatgac caatatgtcc atcttcttcc acgacagtat cctgctcggg
    29581 aaaaccgtgc ataacgcaat tatcctgaat gttcgcgcca tctttcacca cgatacgacc
    29641 aaaatcgcca cgcaggctgg catttggccc aacgtaaacg cccttgccta gaataacatc
    29701 gccgatcaat accgctgtcg gatggacaaa actctcttct ggcacaaccg gagtcagacc
    29761 atctatctgg taaattggca tatcgcctcc tgttcaatat tcaaaccgcc aaaacgttga
    29821 aaatacaggc ttcccggcgc aggcagttca ccgaccgagg tttcaccttt ttcactaacg
    29881 aacgctaaag cagcaggcgc tacgcgctga taaatgttga tacacagctg acgcgccgta
    29941 tgccctgccc agtgcgccgg aagcaactcc tctggcaaca atgggtcttt aaggacgaca
    30001 cggcgataaa aatggatcag taaaagctga atatgaaatg cacgctccgg ggttaactcg
    30061 tctgctgcct cttttaacag cggcaccagc gggcggaatg actgaataaa ggtttcgtac
    30121 atggcatttt gttcagttaa atgccagcac tcttccactc tggcacgcag tgctgcacga
    30181 gaaagcgcca gtggtatttg cgcttcaaaa caaatcacgt tatccgccac ccccgcttca
    30241 tgcaaaagtg tctgtacatc cgccagtttt tgcgacggcg atgccatcag gctgggtgcc
    30301 agtgcgccaa aaccttgcca gatcaactgc tttttgacat cagccagcgt tgatttatct
    30361 aaaccttccg agagcaacag gagccattta ccatcccatg caggttgctc tgcgcgataa
    30421 attttacttt ctgcccgtcg cgtcaggcgc aagcctttat cactgaggct atagaaactg
    30481 cgtcgcccga tgcgggaaac atccagccag ccttctttat taagacgaaa caaagcggtg
    30541 cgcacgaaac gctcaccaaa tcccagccct tccagcaaag cagccagact acccagccag
    30601 atttcaccac cacgatggga aagcgaatca ccatacagag aggagatcaa agatgtgcca
    30661 ctgaccggaa cagcgtttac agcatgttgg ataaaagtaa caagtttact cattcgattc
    30721 agtctgttgt tattgttgtg atttatgaat catagcacag gtcgtatcga cttctggccc
    30781 tggcgcttta cctcaccagg gccagacgac acgtcaggca ccaacaatat tgcgcagatc
    30841 aaacacccga cacgccttgc cttctgaacg cgggatactg ccacagttaa cgatcatcac
    30901 atcggtagag atccccacca tcgacttaat ccgatggcgc agctggtggc acacctggca
    30961 acgctgctca tgcgtcaatg tcaggctgct ttctttcaac tccactttca cagaaagtga
    31021 atcaagatgc ccccggcggt tcacctccag ttggtaatgg ggcgataaat gttcgaattt
    31081 gacaatctct tcttccagtt gtgacgggaa gacatttaca ccgcgaatga tcaacatgtc
    31141 atcgctgcgt ccgctgatgc gatccatgcg gcgcatagtc cgcgccgttc ctggtaacag
    31201 tcgggttaaa tcacgcgtgc ggtaacgaat gaccggcaat gcttctttgg tcagcgtggt
    31261 gaataacagt tcgccatgtt caccatcggc aagcggtgtg ccgtcatgag gattaacaat
    31321 ttcaggatag aaatgatctt cccaaatggt tgggccgtcg gtagtttcca gacactccat
    31381 cgccaccccc ggccccatca cttctgacag gccataaata tccagtgcgg tgatccccag
    31441 acgccgttca atctctttac gcatggcctg tgtccacggc tcagcaccaa acactccaac
    31501 ccgcagcgag caaccgctgg catcaccgcc caactgccgc tccagctctt caatcaggtt
    31561 aaggcaataa gatggcgtaa ccatgatcat atctggttga aaatcgcgga tcagttgcgc
    31621 ttgtttttcc gtctgcccac cagacatcgg gatcaccgta gcgcccagac gttcggcacc
    31681 atagtgcgcg cccagcccac cagtaaacag gccgtaacca taggcaacat gaattttgtc
    31741 tttgggcgtg ccacctgccg cacgcaagga acgcgctaca atattggccc agttatcaat
    31801 atcgttttgc gtatagccga caactgtcgg tttacctgtg gttcccgaag aagcatgaat
    31861 gcgcaccact tgttccatcg gcacggcaaa ggtgtcgaag ggatagttgt cgcgcagatc
    31921 ctgtttggtg gtacagggaa atttacgcag atctgaaagt tccctgaaat catcaggatg
    31981 tacgcctgct gcgtcaaatt tgcgccgata catcgggaca ttttcatacg cgtgtttgag
    32041 cgtccatttc agacgttgtg tttgcaacgc ctgtaactca tcaacagacg cggtttcaat
    32101 cgggtcaagc tttgtattgg ttatcattgt agggtactcg caggttgata tgctcaaaca
    32161 cgctccagaa tcatggcgat gccctgaccg acaccgatgc acatggtgca caatgcgtaa
    32221 cgaccgttac gccgatgcag ctcatggctg gcagccagtg ccaggcgggc accactcatt
    32281 cccaacggat ggcctaaggc gatagcgcct ccgttggggt taacatgtgg ggcatcatca
    32341 ggcagcccca attcgcgtag tacacccaac gcctgggccg cgaacgcttc gttcagttca
    32401 atcacgtcca tatcgtgaat actcagcccc gcgcgttcca gcacccggcg agttgcaggc
    32461 accggaccaa gccccatcag gcgcggttcc accccggcgg ttgccatggc tacgattcgc
    32521 gcccgcggtg tcagtccttg cgctgctgcc atctgttcac tggcaataat caacgcagcg
    32581 gctccgtcat tcaccccgga ggcattgcct gcggtaatca ccccattggc acgaaatggt
    32641 gcttttaacc cacgtaactg ttccagcgtc gtttccgggc gcagatgctc atcatgttgt
    32701 atttctgtta caacgccttt cttgtttttc aacacaaccg gaacaatctc ctcagccaga
    32761 atgcctgagg attgcgcttt tgccgtacgt tgctgactgc gtagcgcaaa actatcttga
    32821 tcttctcgcg atatttttaa cagttcagct acattctctg ccgtttccgg catgctgtca
    32881 gttccaaatt gttgagccat gagcgggttc acaaatcgcc agccaatagt ggtatcaaac
    32941 atctcagcct gacgagaaaa tgcactggtt gccttgccca taacaaacgg tgcccgagac
    33001 attgactcca cgccaccagc gatcagcaaa tcgccatcgc ccgctttaat cgcccgtgcg
    33061 gcgaacccca gtgcgtccag cccggaacca cacaagcggt taatggttgt gccggaaaca
    33121 ctctgcggca gccccgccag taacgtcgcc atccgggcta cgttacggtt gtcttctccc
    33181 gcctgattag cacagccgag gatcacatca tcgatacact ccgcatcgag acgcgggttt
    33241 cgcaccagca gctcccgcaa agggatagca gccagatcgt cagcccgaac acctgataat
    33301 gccccaccat agcggccaat tggcgtacga attccgtcac agataaaggc ttcacgcatc
    33361 aggcttctcc tgtaatggtg ccgccgatgc ggtgagattt accgcgaaac agcgcaaccg
    33421 ttttttgttg ttggttaaca atttcgatgt cgtaaacacc ggtttgcttg ccctggtgac
    33481 gtacctgcgc agtagcagtt aaggtgtctc cggcaaagcc tggacgcaaa aaatcaatcg
    33541 tgcaggcaga agcgacggct gccagcccct ggctattgca ggcgtaggca aaggcagtat
    33601 cagccagtga aaatagctgc ccgccgtggc aactttgatg accgttaagc atttgtgcag
    33661 tgacggtcat ggtcactaca gcaaagcctt catccattga gataatgtcg atgccaagcg
    33721 ctttggcgca ggcatcgttc tcatacattg catgggcatt ttgccaggcc ttatgactca
    33781 taaccgctct ccagaagcgc tcgttggcgc agcaatgaac atgggcgata gcgttcttcg
    33841 ccatagtgat gttgtagatt ttccaggagc cttaatattc gctgccagcc aagttgcgct
    33901 ccccaggcaa gcgggccata tggataattc accccaagac gcatggcggt atcgatatcc
    33961 tgttcagagg ccacgccttt ttgcagcgca tcaagggctt cattgatgat cattgctacc
    34021 gttcgccaaa tcagcattcc tgggtaatct gcaatttgca gcactgtttt gccctgctgt
    34081 tgcaggtaat aaatggcctt gcgggtcgct gagtccgggt tcaccgctgc cgcagcaatg
    34141 gtcaccacct tgcccgccat tttgtcgacc actaccaccg ggcgtgccag tcgtattgcc
    34201 agcgcctgtg ccgtctcgcc ttgtgtctca atcaataaaa catcgtcaat ttccgtgacg
    34261 ccgtcacttt tcttttctac tttcattggg ctaaaactgt cgcttaccgc ttccaggcca
    34321 acaactgcct cacgttccgc gcgccagtcg tacacgccca gcccactttt cttgcccaac
    34381 cgtccaccaa tcaccagttc ctgttgcacc agcgaaggta aaaaacgacg ctcctgccag
    34441 aaagcgttaa acaccgaaca ggtgacagca aaattgacgt cctgaccaat cagatcggtt
    34501 aattccagcg gccccatcgg gaaaccagcg ccatcgcgaa gtgcagcgtc aatcacttct
    34561 ggtgcatata gcgcggcgat ttttttgata ccgatttgca tttcatttga taccgctgaa
    34621 aaacgccgat acgcaactta tttgatacta aatcggcgct tttcttttga gttttagaat
    34681 gagtttttaa gcgctcattc actctctttc aaaccagttt taactgtttg tggttttggg
    34741 taaggctgga ctttgaatag ctttgtgttg aggcgttttc tggccctctt gttcagaaac
    34801 cggatgtaac gaaactgtcg gaacttatga acactagctc tgtcaatatt agccctcaga
    34861 tgttcacctc gttgtccgcc tcgtttaatg gcattcctgc aaatctcgtg ataccactcg
    34921 ccatcaagtt catagaatat tgattcatgg ctgcctacat aatcaaaatt gcttgcctga
    34981 tacacgacac caagacagcc acaacgctca tctgcaaacg actgaaccca ctgtacctgc
    35041 ggataaagct g
//
LOCUS       DAZFVO020000034        34332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFVO020000034 DAZFVO020000000
VERSION     DAZFVO020000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.034"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..326
                     /locus_tag="ACHKNB_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /pseudo
     CDS             189..326
                     /locus_tag="ACHKNB_RS07750"
                     /old_locus_tag="pgaptmp_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(434..1669)
                     /gene="aslB"
                     /locus_tag="ACHKNB_RS07755"
                     /old_locus_tag="pgaptmp_001551"
     CDS             complement(434..1669)
                     /gene="aslB"
                     /locus_tag="ACHKNB_RS07755"
                     /old_locus_tag="pgaptmp_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4195091.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1816..1892)
                     /locus_tag="ACHKNB_RS07760"
                     /old_locus_tag="pgaptmp_001552"
     tRNA            complement(1816..1892)
                     /locus_tag="ACHKNB_RS07760"
                     /old_locus_tag="pgaptmp_001552"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Pro,seq:tgg)
     gene            complement(1935..2021)
                     /locus_tag="ACHKNB_RS07765"
                     /old_locus_tag="pgaptmp_001553"
     tRNA            complement(1935..2021)
                     /locus_tag="ACHKNB_RS07765"
                     /old_locus_tag="pgaptmp_001553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1985..1987),aa:Leu,seq:cag)
     gene            complement(2042..2117)
                     /locus_tag="ACHKNB_RS07770"
                     /old_locus_tag="pgaptmp_001554"
     tRNA            complement(2042..2117)
                     /locus_tag="ACHKNB_RS07770"
                     /old_locus_tag="pgaptmp_001554"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2082..2084),aa:His,seq:gtg)
     gene            complement(2176..2252)
                     /locus_tag="ACHKNB_RS07775"
                     /old_locus_tag="pgaptmp_001555"
     tRNA            complement(2176..2252)
                     /locus_tag="ACHKNB_RS07775"
                     /old_locus_tag="pgaptmp_001555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2216..2218),aa:Arg,seq:ccg)
     gene            complement(2355..3740)
                     /gene="yifK"
                     /locus_tag="ACHKNB_RS07780"
                     /old_locus_tag="pgaptmp_001556"
     CDS             complement(2355..3740)
                     /gene="yifK"
                     /locus_tag="ACHKNB_RS07780"
                     /old_locus_tag="pgaptmp_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4195092.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3931..4671)
                     /gene="wecG"
                     /locus_tag="ACHKNB_RS07785"
                     /old_locus_tag="pgaptmp_001557"
     CDS             complement(3931..4671)
                     /gene="wecG"
                     /locus_tag="ACHKNB_RS07785"
                     /old_locus_tag="pgaptmp_001557"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HGU4195093.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4674..6026)
                     /gene="wzyE"
                     /locus_tag="ACHKNB_RS07790"
                     /old_locus_tag="pgaptmp_001558"
     CDS             complement(4674..6026)
                     /gene="wzyE"
                     /locus_tag="ACHKNB_RS07790"
                     /old_locus_tag="pgaptmp_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HGU4195094.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6023..7102)
                     /gene="rffT"
                     /locus_tag="ACHKNB_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /gene_synonym="wecF"
     CDS             complement(6023..7102)
                     /gene="rffT"
                     /locus_tag="ACHKNB_RS07795"
                     /old_locus_tag="pgaptmp_001559"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HGU4195095.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7099..8349)
                     /gene="wzxE"
                     /locus_tag="ACHKNB_RS07800"
                     /old_locus_tag="pgaptmp_001560"
     CDS             complement(7099..8349)
                     /gene="wzxE"
                     /locus_tag="ACHKNB_RS07800"
                     /old_locus_tag="pgaptmp_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HGU4195096.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8351..9481)
                     /gene="rffA"
                     /locus_tag="ACHKNB_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8351..9481)
                     /gene="rffA"
                     /locus_tag="ACHKNB_RS07805"
                     /old_locus_tag="pgaptmp_001561"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HGU4195097.1"
                     /translation="MIPFNAPPVMGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9486..10160)
                     /gene="rffC"
                     /locus_tag="ACHKNB_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /gene_synonym="wecD"
     CDS             complement(9486..10160)
                     /gene="rffC"
                     /locus_tag="ACHKNB_RS07810"
                     /old_locus_tag="pgaptmp_001562"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HGU4195098.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10138..11019)
                     /gene="rfbA"
                     /locus_tag="ACHKNB_RS07815"
                     /old_locus_tag="pgaptmp_001563"
     CDS             complement(10138..11019)
                     /gene="rfbA"
                     /locus_tag="ACHKNB_RS07815"
                     /old_locus_tag="pgaptmp_001563"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGU4195099.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11038..12105)
                     /gene="rffG"
                     /locus_tag="ACHKNB_RS07820"
                     /old_locus_tag="pgaptmp_001564"
     CDS             complement(11038..12105)
                     /gene="rffG"
                     /locus_tag="ACHKNB_RS07820"
                     /old_locus_tag="pgaptmp_001564"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGU4195100.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12105..13367)
                     /gene="wecC"
                     /locus_tag="ACHKNB_RS07825"
                     /old_locus_tag="pgaptmp_001565"
     CDS             complement(12105..13367)
                     /gene="wecC"
                     /locus_tag="ACHKNB_RS07825"
                     /old_locus_tag="pgaptmp_001565"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HGU4195101.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13364..14494)
                     /gene="wecB"
                     /locus_tag="ACHKNB_RS07830"
                     /old_locus_tag="pgaptmp_001566"
     CDS             complement(13364..14494)
                     /gene="wecB"
                     /locus_tag="ACHKNB_RS07830"
                     /old_locus_tag="pgaptmp_001566"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HGU4195102.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14550..15596)
                     /gene="wzzE"
                     /locus_tag="ACHKNB_RS07835"
                     /old_locus_tag="pgaptmp_001567"
     CDS             complement(14550..15596)
                     /gene="wzzE"
                     /locus_tag="ACHKNB_RS07835"
                     /old_locus_tag="pgaptmp_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HGU4195103.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15608..16711)
                     /gene="wecA"
                     /locus_tag="ACHKNB_RS07840"
                     /old_locus_tag="pgaptmp_001568"
     CDS             complement(15608..16711)
                     /gene="wecA"
                     /locus_tag="ACHKNB_RS07840"
                     /old_locus_tag="pgaptmp_001568"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HGU4195104.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16951..18210)
                     /gene="rho"
                     /locus_tag="ACHKNB_RS07845"
                     /old_locus_tag="pgaptmp_001569"
     CDS             complement(16951..18210)
                     /gene="rho"
                     /locus_tag="ACHKNB_RS07845"
                     /old_locus_tag="pgaptmp_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HGU4195105.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18295..18396)
                     /gene="rhoL"
                     /locus_tag="ACHKNB_RS07850"
                     /old_locus_tag="pgaptmp_001570"
     CDS             complement(18295..18396)
                     /gene="rhoL"
                     /locus_tag="ACHKNB_RS07850"
                     /old_locus_tag="pgaptmp_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HGU4195106.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18537..18866)
                     /gene="trxA"
                     /locus_tag="ACHKNB_RS07855"
                     /old_locus_tag="pgaptmp_001571"
     CDS             complement(18537..18866)
                     /gene="trxA"
                     /locus_tag="ACHKNB_RS07855"
                     /old_locus_tag="pgaptmp_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HGU4195107.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18997..20262
                     /gene="rhlB"
                     /locus_tag="ACHKNB_RS07860"
                     /old_locus_tag="pgaptmp_001572"
     CDS             18997..20262
                     /gene="rhlB"
                     /locus_tag="ACHKNB_RS07860"
                     /old_locus_tag="pgaptmp_001572"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HGU4195108.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20396..21880
                     /gene="gppA"
                     /locus_tag="ACHKNB_RS07865"
                     /old_locus_tag="pgaptmp_001573"
     CDS             20396..21880
                     /gene="gppA"
                     /locus_tag="ACHKNB_RS07865"
                     /old_locus_tag="pgaptmp_001573"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HGU4195109.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21927..23948)
                     /gene="rep"
                     /locus_tag="ACHKNB_RS07870"
                     /old_locus_tag="pgaptmp_001574"
     CDS             complement(21927..23948)
                     /gene="rep"
                     /locus_tag="ACHKNB_RS07870"
                     /old_locus_tag="pgaptmp_001574"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HGU4195110.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24165..24614
                     /locus_tag="ACHKNB_RS07875"
                     /old_locus_tag="pgaptmp_001575"
     CDS             24165..24614
                     /locus_tag="ACHKNB_RS07875"
                     /old_locus_tag="pgaptmp_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4195111.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24813..25094
                     /gene="ppiC"
                     /locus_tag="ACHKNB_RS07880"
                     /old_locus_tag="pgaptmp_001576"
     CDS             24813..25094
                     /gene="ppiC"
                     /locus_tag="ACHKNB_RS07880"
                     /old_locus_tag="pgaptmp_001576"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HGU4195112.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25181..26656)
                     /gene="ilvC"
                     /locus_tag="ACHKNB_RS07885"
                     /old_locus_tag="pgaptmp_001577"
     CDS             complement(25181..26656)
                     /gene="ilvC"
                     /locus_tag="ACHKNB_RS07885"
                     /old_locus_tag="pgaptmp_001577"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGU4195113.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26806..27696
                     /gene="ilvY"
                     /locus_tag="ACHKNB_RS07890"
                     /old_locus_tag="pgaptmp_001578"
     CDS             26806..27696
                     /gene="ilvY"
                     /locus_tag="ACHKNB_RS07890"
                     /old_locus_tag="pgaptmp_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HGU4195114.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27693..29237)
                     /gene="ilvA"
                     /locus_tag="ACHKNB_RS07895"
                     /old_locus_tag="pgaptmp_001579"
     CDS             complement(27693..29237)
                     /gene="ilvA"
                     /locus_tag="ACHKNB_RS07895"
                     /old_locus_tag="pgaptmp_001579"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HGU4195115.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29240..31090)
                     /gene="ilvD"
                     /locus_tag="ACHKNB_RS07900"
                     /old_locus_tag="pgaptmp_001580"
     CDS             complement(29240..31090)
                     /gene="ilvD"
                     /locus_tag="ACHKNB_RS07900"
                     /old_locus_tag="pgaptmp_001580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGU4195116.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAANGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31155..32084)
                     /gene="ilvE"
                     /locus_tag="ACHKNB_RS07905"
                     /old_locus_tag="pgaptmp_001581"
     CDS             complement(31155..32084)
                     /gene="ilvE"
                     /locus_tag="ACHKNB_RS07905"
                     /old_locus_tag="pgaptmp_001581"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HGU4195117.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32104..32367)
                     /gene="ilvM"
                     /locus_tag="ACHKNB_RS07910"
                     /old_locus_tag="pgaptmp_001582"
     CDS             complement(32104..32367)
                     /gene="ilvM"
                     /locus_tag="ACHKNB_RS07910"
                     /old_locus_tag="pgaptmp_001582"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HGU4195118.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32364..34010)
                     /gene="ilvG"
                     /locus_tag="ACHKNB_RS07915"
                     /old_locus_tag="pgaptmp_001583"
     CDS             complement(32364..34010)
                     /gene="ilvG"
                     /locus_tag="ACHKNB_RS07915"
                     /old_locus_tag="pgaptmp_001583"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HGU4195119.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34013..34063)
                     /gene="ilvX"
                     /locus_tag="ACHKNB_RS07920"
                     /old_locus_tag="pgaptmp_001584"
     CDS             complement(34013..34063)
                     /gene="ilvX"
                     /locus_tag="ACHKNB_RS07920"
                     /old_locus_tag="pgaptmp_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HGU4195120.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34150..34248)
                     /gene="ilvL"
                     /locus_tag="ACHKNB_RS07925"
                     /old_locus_tag="pgaptmp_001585"
     CDS             complement(34150..34248)
                     /gene="ilvL"
                     /locus_tag="ACHKNB_RS07925"
                     /old_locus_tag="pgaptmp_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HGU4195121.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         8311 a         9441 c         8673 g         7907 t
ORIGIN      
        1 ttacatatca actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt
       61 ttctggataa ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg
      121 agagggatct caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag
      181 cattttgcat ggaattttcg ttttcaccca aacttcttgt tgttgctgtc gccgccgctc
      241 ttcctctcat ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg
      301 ccggatacga tcacccaaac cagtatccac agaacgctgc aatttattga atttgcacga
      361 tcatgtaggc cggataaggc gtttacgccg catccggcaa tcaaccgcag gcggccacaa
      421 aatggccgct tttttactta ttcaccacca gcaaatgtgc ctgcataatg tcactggccg
      481 ggcgaccatg cgccagcaaa tcagccattg ctttaagata tggcggtaga tggcggaaat
      541 aacgctgata cccggcacac aaataattca gccccggttt gccgctggca tcgagcatga
      601 agcggtgttt cgggcagcct ccccagcacg cttttaacac gttacaactg cgacactgcg
      661 ccggtaattg cttaaattta tcttcaccaa acacctgctg ttgcggggaa tcgatcattt
      721 ctgcaattgt ttgctgatgc atattcccca gccgatattg cggataaaca tagtgatcgc
      781 aggcgtaaac gtcgccgttg tgctcaacaa tcaccgagcg cccacaggtt ggctgatgat
      841 ggcaaaccgc acccggcgca ccgacaaaat tggcaaacgc ccattcgata ttcatcacga
      901 aaatcttgcc gacgtcgcgt ttgatccagt ggtcgaatat cgccaccaga aattcaccaa
      961 attcatcggg gcgcaccgac cattccgtca gttccccctg aatatcacca ggcgcatgta
     1021 gtttcagtcc atcgctggca gctgtttcat cagccaggcg ctcgaccacc ggaataaact
     1081 ggatgaattc gactcccgca tcgcacaaaa aatcataaac ctgcaacggt tgctgcgcgc
     1141 tggtgcggtt gacgcagacc agcacgttat agtcgacatg atgtttttgc agcagcgtca
     1201 gggcacgcat caccagctta tgcgttggtc tgccaccttt ggtcacgcga tattgattgt
     1261 ggatctcagc cggaccgtcc agcgataacc caacaagaaa atgattttct gccagaaatg
     1321 cacaccattt atcatcgagc agcacgccgt tagtctggaa gctgttactt atcttcctgc
     1381 cagcaccgta tttcgcctgt aactttacgg cacggcggta aaaatccagc ccgagtaacg
     1441 ttggttcgcc gccctgccag gtaaaagcca cttcgttttg cggttcgctg gcagcgatat
     1501 agtgacggac atacgcttcc agcgtgtcat cgtccatatg cgtgactggc ttttcgcggt
     1561 aaagggattg tttttcgaga taaaaacagt agtcacagtt cagattacaa tcggaaccac
     1621 tcggtttcgc catcacatga aaagcacgcg ttggaacctg ttgcagcatg gtcactcctt
     1681 gcctgaagtc atccttcaat acgttaaggg cgaaatcata ccgtggggca atgagttatt
     1741 acgggcgttt attgcgaagg gagggtgacg aaatgcacag aaaacaaaaa accccgccga
     1801 agcggggttc aaaattggtc ggcgagagag gattcgaacc tccgacccac tggtcccaaa
     1861 ccagttgcgc taccaagctg cgctactcgc cgaaatactg ctttttgaat ttttagttca
     1921 attctttaaa gtcgtggtgc gagggggggg acttgaaccc ccacgtccgt aaggacacta
     1981 acacctgaag ctagcgcgtc taccaattcc gccaccttcg cattgtcaca acttctaata
     2041 atggggtggc taatgggatt cgaacccacg acaactggaa tcacaatcca gggctctacc
     2101 aactgagcta tagccaccac tacaaatctt gttacgcggt attactacca ccgcagctca
     2161 agcgccggga ctaaatggcg cgcccgacag gattcgaacc tgagacctct gcctccggag
     2221 ggcagcgctc tatccagctg agctacgggc gcttagcgcc gttgcggggg tggataatac
     2281 ggacttccca cccctctgtc tagtcccttt ttaaataaat tgcgcgtttg gttacggttt
     2341 gtgcgttttg ctgcttattc ctccagttta tgcgctttcc cgtggcgatt aaggccaaaa
     2401 actttataaa tcgccgtcac cgccagcata aagatgatgc caacaaacag cgacatacgc
     2461 gtatcttcat taaagtacat gccgatcaaa acgcaaatca ggaatgccat tgttacgtag
     2521 ttggcccacg ggaacaggat tgagcggaac ggatggctgg caatcgccgc tttatgtgca
     2581 cgccgaaaac gcagctggct tatcaatatc acaaaccatg gcaccatccc cggaagcacg
     2641 ctggcactgt agacgtagac aaacacacgc tgcggattgg gaatgatgta gttcaggcat
     2701 gagccaatta gcagaatagc aatagatact gccacacccg caactggtac gccgtgacgg
     2761 gaaactttcg ccatcgccgc cggtaactga cggtttttcg ccagtgcgta gagcatacgt
     2821 ccgcaactgt acatgccgct gttacagcca gagagcgcag ccgtcagcac cacaaagttg
     2881 ataatgcccg ccgctgcggt aataccgatt ttggcaaaag tcagtacgaa cgggctgccg
     2941 ttgctgccaa tttcattcca cgggaagatg gtgacgataa cgaaaatcgc gcctacgtag
     3001 aaaatcagga tccgccacag cactttgcct acggcactgc gcagcgtcac ctgcggattc
     3061 ttcgcttcac cggcagtaat gccaatcagc tccacgccct ggtaggacgc caccacaata
     3121 cacagcgcgg tcaggaaccc tttccagcca cccgcaaaga aaccgccatg ctctgtgaga
     3181 ttgctaaaac caatcgactg cccgccattg ccaaagccaa agaaaatcac gcccaggcca
     3241 atgacaatca tcacgataat cgtggtgact ttgatcatcg cgaaccagaa ctcgatttcg
     3301 ccgtacaacc gcaccgccgc cagattcgcc aacgccacca gcgccactgc gatcaatgcg
     3361 ggtatccact gcgccatctc cgggaaccag aactggacat aaacgccaat ggcggtgatt
     3421 tctgagatcc ccacggccat ccacataaac cagtaagacc aggcggtgag ataaccaaag
     3481 aacgggctca tataacgatg cgcataaacg gcgaacgaac cggtaaccgg ttcgaggaac
     3541 aacatttcgc ccattgaacg catgatgaaa aagacgaata gcccggcgat gatataggcc
     3601 aacaatacgg atggcccggc ccatttcaag gtactggcgg cccccataaa caggccgacg
     3661 ccaatggtgc cccccagggc gatgagttcg atatgtcgag cttccagccc acgctgtagc
     3721 tctggtttgt tatctgccat aaatcctcgt gttgtgtttg catgctttcc ggtgttaccg
     3781 gttatcgtta tgggtacatc gagtgttgcg aatgttttcg taattcagga gaaatggcaa
     3841 ataaagcatt aaaaatttga atgctttgtg taataaaaaa gcagacaggc gacggagtga
     3901 ccactccgtc gctttacaaa gagaggaaaa tcagaggttg ccggtgtagt gccagcgtaa
     3961 ataacgcagc aaacgaagct gacgcttaat gcggctcggc tgcgaaagca ggcggtagag
     4021 ccactccagt cccagcgttt gccagatttt cggtgcgcgt tttacgtgac cggtgaaaac
     4081 atcgtaagtc ccgccaacgc ccatatacag cgcatctgga tgcaccagac ggcagtcgcg
     4141 catgaagatc tcctgctttg gcgatcccat cgcaacggtg acgatttgcg ctccgctggc
     4201 atgaatgcgt tcaaacagcg cctgacgctg ctcgggttta aaataaccat cctgactgcc
     4261 aacgatattc acattccatt ggttgcgcag tttagcttca gtttgcgcca gcacttcagg
     4321 tttaccgccc acaagaaata ccggcgtccc ttctttgcct gcgcgcgcca tcagctcttc
     4381 ccagagatcg gcaccggcaa cgcgggaaac ctgcgcctgc gggtactttt tacgtactga
     4441 acgtacaacg ctgatgccat ccgcatattt aaattcggca gcgttaatta actccctgac
     4501 ctcggcgtta tcttcaatgg tcagcatttt ttcggcatta atggcaacca gcgttccctg
     4561 cttaagctgc ccgtcagcaa acagataatc gagggcgtgc tgcatatcac gccaaccaat
     4621 caactgtaag ccacgcagcg tataggtcgg tgccgtggtg ttgttattca ttgttatcct
     4681 tcaacctgcg tccggagcga tgattttgta cgtttatgaa tgagtccggc gctttcaaaa
     4741 agccagtaca acagttttgc gatcatcaga catgcgccga agaccacgat aaaaaagact
     4801 acgcgtgaga caaacgaatc cagcccttca cgtgccagca cgatcatatt gaagatcgcc
     4861 ccaaagcaga aactgtgcaa tatcgcagcc ttatagcgat taggctcgcg gttgcccagc
     4921 tcatacagcc agtcgaacca tttgatgatc agaccaacca cgatcgcccc gagcgggatg
     4981 aacaacgcgc cgcccatcac caccagtgag cctataagcg taggcgagat cgccagtccg
     5041 gagtggttat tcagcacttc ccaggtaaag tagttggctg agttcagcac catactcggg
     5101 cgacccggcc acagccagga agggataaag acatagaaat cgcggacaat tggagccagg
     5161 ccctggaagt cgatgttgtc gtagttctgc aacagcaacg ccagattctc ccacggcgag
     5221 aaggtgtcgc gagtgagata gagaaacgta tagaacgctt catcgccgct cacattcatt
     5281 ccatagcgtt ttagcgccag ccagaacatg cccacaatcc ccagcacgcc cgccgccgcc
     5341 agcatccaca acgaaatcca gccgcgaata atgccaataa acaggaagat agcgaatgcg
     5401 atgatgatat tggcgcgagt gccgccgaca atcatataag tcagcaagcc aaaggcgacc
     5461 gtgctgacga ggaaaaacag ccacgctttg ctatcctggc gcagaaagta gaccaccagc
     5521 atcgccggga tgaaaaagta aaagaagcgt tttaacgcca cgccggagac ttcactggag
     5581 aagatctgac tgtaggagtt aagccggaac agcaaaaagc cgttgtgcat gaagaagatg
     5641 ccgacgctta ccagcgcgat gcccatcagg atcacccacg taagattggt ctccacgcgg
     5701 ttcatggtaa acagcggacg gcgcggtaca tcagcaacgc gtttgcgtag gcgggttttg
     5761 taggtgacat agtaaaccgc gtagaagcag cccgcagaaa gcaacgcctg caacaagatt
     5821 tctggcggcg cgacaccaac atcaaagcga aataccagca cgctggtcag cgggaagccg
     5881 aagaaaaagg tgagcaaaaa cagcaatgaa aagaagacat tgaagttaaa gcgcacacgg
     5941 cgaaactcaa accaggtcag cgtggcaata aacagcgtgc agagcagcca gaccacaaac
     6001 aggccactga attgcagcag actcatgcgc cctccccggc ggcaatcgcc aacgcccgct
     6061 gccagccttg tagatagtta gggctaaaga aggcaatggt gtttttatcc accgacgcca
     6121 actggcgctg cgcttcacgc acaatatcct cgttgagatc gtcggtagta aacagcactg
     6181 gcaaatgctg ttccgtcata tcctgccaga acggattttc ccggttaagc acacaaggaa
     6241 tgcccgcctg aatcagtaag cacagcgtac caatgccctg ctggcgagca aaaataaagt
     6301 aaccaagatc gcactgacga agtagcgcca gataggcgtc aaattccagt ttttcgctca
     6361 aaatttgcag attttcttcg ctgaataact ccagccccgc ctgacgaact tcctcaatgt
     6421 acgcttcgtt attaggcgga tatcccatcg gcaccaccac ttttaccgta tcgccaaatt
     6481 gctgatgaac ggcacgtaaa gcggcgacat gctcattgct gaggtcgccg gagttcccca
     6541 ccagaatggt catttttcct tcacgttgcc gatcgttcgc catcgtattg agagaagggt
     6601 ccatccgcgt cgggaagtac agcagttctc cccgcacctt tgggtgcgtt ttggcaaaaa
     6661 agctcaaatc gccgcgggtg gcaaatacac agccgactcg cttttgcgcc aggcgacgta
     6721 gtgggtaaaa aagcttatat ctcaagccac tggaaagctc atacaggtct gccccccaga
     6781 tatgccagaa aaactggctg ggcttaatgc caccactcag cagagccagc cacagtgtgg
     6841 gattgaactg accgtggaaa aaaaatcgct gctgacggtt agcttttgct ttcgcgatga
     6901 ccgcttccgc cagcgatttt ttcccaggga aaaattgcac agaaagcgcc ggacagctat
     6961 cacttaagcc gtcgtccttg ccaacaacca taaactcgcg cgcgtgctcg ctcgtcgcag
     7021 ccagcgcgtc attgaaaaac cgcaaaacgg ttcggttatg gtgagggata tccgatccca
     7081 gtacgtgaat cagtacagtc atgcccgcct acgccagagt aaaaacacgc cacaacaaag
     7141 agaaaaatag acgatataag ttgccatata tgcctgcgcc gcgcccagtg caccatgcgc
     7201 agggattagc cagtgggcaa ataccatcaa taaagtgaac tggctgattt ccgccagaat
     7261 ataaaaccgc aatgacgctt tggcgatcac cagataacca aagacataag cgcccacttt
     7321 taacacatca cccactaact gccaggcaaa gagatcgcgc atagcggtaa atttattcga
     7381 caacagcagc cagatagcaa aatcacgcag cagccagacg gtaaaactcg ccgccgccac
     7441 tgccggtaag acgaatttca gcgatttaac cacttcccgg gtgatatcgc gcttttccgt
     7501 tagccgcgac aacgtgggca gcaaatatac gctgaacgat gccgtaataa attgcaggta
     7561 ggcatcggaa atactgctca ccccttgcca gatccccact tcatcccagc tatactgcgc
     7621 cgccagcagt ttacgcatca tgatgtaagc aacaggcaag gtcaccgacg taatcaacgc
     7681 catgagcgta aatttgctca actgccctgc cagaccgtta tcccagctgg gtttcagata
     7741 gcttaacggg atgacaccac gtttgatcaa catgatagcg gcaggaatta ccaccagcgc
     7801 gggaatcagc gccagaccca gcaacgcccc ttcataaccg cccaaacggt aactgacgta
     7861 gtacgcgaga acgccaatca agctgccgac aatcagcgat aacgcattac ctgcggcatc
     7921 gcgaaagcct ttcatcagcg ccagtaacag gttgccccag gcgatcccca tttgcaccag
     7981 cgccactaaa cgcaccagcc cctgatagtc ggtattacca aacaatccct ggctgattgg
     8041 cgcagctgcc agcacaaaaa ccagcgccat cagcgtagag aaaccaagta ccatcgctga
     8101 tgaagtgccg accacgcggc gcagctgttg cggattatca tggtactggg caacgtattt
     8161 ggttacaccg ttaaagatgc cagctccggc aagcacgccg agcacggtaa tcaactggcg
     8221 gaaatttgcc gccagcccaa gccccgccgg accaaatgac acagccagca acttaccgac
     8281 cagtaacccg gcaccaatct tgaccagtgt actggccgcc gtccacaagg acgcttttgc
     8341 caacgacata tcaggagaag tagttcaaca aagtcgcaat taccgtacgc tgattgacgg
     8401 gcgacaggtt gtagaacagc ggcaggcgca gcaggcgctc gctctctttg gtggtgtagc
     8461 gatcttcacc gtggaactca ccaaagcgtt cccccgcagg gcaaccgtgc agcggaatgt
     8521 aatggaacac cgccatgatt tccgcttctt tcagaaagtt aatcaacgcg ctccggtcat
     8581 caatatcccg cagtttaatg tagaacatat gcgcgttctg cacgcagcca tcgggaatcg
     8641 acggcagctc gatacgcccg gctttcgcca gaggcgctaa cgcatcgtag tagttttgcc
     8701 acagcgccag acgttgctgg ttgatacgat ccgctgcttc cagttgcgcc cacaggtacg
     8761 cagcttgcag atcggacatc aaatagctgg agccaatatc gcgccaggta tatttatcga
     8821 cctgaccacg gaagaactgg ctgcggttag tgcccttttc acggatgatc tcggctcgtt
     8881 cgattaacgc tttatcgtta atcagcgtcg cgccgccttc accacccgcc gtatagtttt
     8941 tggtttcatg gaagctaaag cagccaatat gaccaatggt tcccagtgca cgccctttgt
     9001 aagtggacat cacgccctga gcggcatctt ctaccacaaa cagattatgc tttttcgcca
     9061 acgccataat ggtgtccatt tcgcaggcca cacccgcgta atggaccggc acgataacgc
     9121 gcgttttgtc ggtgatcgcc gcttcaatca gcgtttcgtc gatgttcatg gtgtccgggc
     9181 gaacatccac aaaaacgatt tttgcgccac gcagcacaaa ggcattggcg gtggagacaa
     9241 aggtgtagct cggcatgatc acttcatcgc caggctggat atcgagcagc agcgccgcca
     9301 tctccagcga agcggtgcag gacggcgtca gtaacacttt ggcgctgcca aaacgttgct
     9361 ccagccactg ctggcagcga cgggtaaaac cgccatcgcc acacagtttg ccgctaccca
     9421 ttgccgactg catatagtcg agttcggttc ccatcaccgg cggtgcgtta aatggaatca
     9481 tgtgatcacc tgtataacca gtacgcggtg ctttctacat tcgcaccact ttgtatgtat
     9541 cgtttaagcg cggcggtgtt gcccatttgg gtcgccaccc gcaaagttgt tttaccgcga
     9601 gcatacgccc agtttagcgc cgtttgcatc agctcagcac ctgcaccgcg tccagccagc
     9661 aggccaattc gcgcatctgt cgcattgagt tcccgtaaag agacataacc gcgaataccg
     9721 ccggacgccg cacgtaaaat cagacattga tgatcaaagg tgccgcgcac ggcattttca
     9781 atccactgtg cataaaagcg tccgctggcg tcaggcgcat accacggcgc acgaaaacgg
     9841 ctttgcgcaa atgcggcgct ggctaactga cgtaatgcgg gaatatcggt ctcttgtgcc
     9901 actacagcac cactatcact gacattgttc acgggtagtg ccaaatcaac ttcaccttct
     9961 accagggaga atcccagctg ttgcagggca tccagttcac ccgtatttga tgccgcaatt
    10021 ttggcctgca cccgtgacca cggcgctaac acgtctggcg tcaggagcgg tgcttcagac
    10081 gtaatgcgca cgatggcgct gttaacacca aagaaggcgt tttcccaggt tagtggctca
    10141 atactggcgc ggacgggcac gaagtaactc cagcagatat tggccgtagc cagttttcgc
    10201 taatgaactg gcagcacgct tcacaccctc gtcatcgagc cagccgttac gccaggcgat
    10261 ctcttccagg caggcaatct taaagccctg gcgtttttct actgtctgca caaaggtact
    10321 ggcttcaatc aggctgtcgt gagtgccagt gtccagccag gcaaatccgc gcccgagcag
    10381 ttcaacggtc aggttgcccg cctcgaggta catctggttg atggaggtaa tctccagttc
    10441 accacgctcc gacggcttca cctgctttgc gtactccacg actttactgt cgtagaaata
    10501 aagcccggtc accgcccagt ttgacttcgg ctgttttggc ttttcttcca gcgagatagc
    10561 gcggaaattg tcgtcaaact ccaccacgcc aaagcgttcc gggtccatca cctgatagcc
    10621 aaataccgtc gccccttccg tgcgcgccgc aacatgacgc agcttcggac tgaagccctg
    10681 accgaagaag atgttatcgc ccagcaccag acaagaaggt tcaccgttga ggaaggtttc
    10741 accgatgata aaggcctgcg ccagaccgtc cgggctgggc tgttcggcgt attccagctg
    10801 gataccgaac tcactaccat cgcccagcag gcgctggaaa taacctttat cttccggcgt
    10861 agtgatgatg agaatttcgc ggataccggc caacatcagc accgacagcg gatagtaaat
    10921 cattggctta tcgtaaatcg gcaacagttg cttcgatacg ccgcgcgtaa tcggatgcaa
    10981 tcgggtgccg gaaccgcccg ccaggataat acctttcatg tttgcctccg ctgaaaatta
    11041 gcctttcaga cctaaacgct cgccctgata gctgccgtcc tgcacctgct tccaccagct
    11101 ttcattagcc agataccact gcaccgtttt acgcattcca ctttcaaagg tttcctgtgg
    11161 cagccaacca agttcacggg caattttcga agcatcaatg gcataacgca gatcatgccc
    11221 cggacggtca gcgacaaagg tgatcaagtc acgatagtgc gccacgccgt gcggcttgtt
    11281 cggagccagt tcttccagca gctcgcaaat ggtttccaca acatcgagat tcttacgctc
    11341 gttgtgacca ccaatattat aggtttcacc gactttcccg gtggtcgcca cgcaatacag
    11401 cgcgcgggcg tgatcttcca catacagcca gtcacggatt tgctgcccgt tgccatatac
    11461 cggcagcggt ttacccgcca gcgcgttgag gatcatcagc gggatcagtt tttccggaaa
    11521 gtggtaagga ccgtagttat tcgagcagtt ggtgatcagc gtcggcagac cgtaggtccg
    11581 cagccaggcg cgcaccagat ggtcgctact ggcttttgac gcggaataag ggctgctcgg
    11641 cgcatacggc gtggtttcgg tgaagaaatc atccgtcgag tgcaggtcac catatacttc
    11701 gtcggtggag atatgatgaa aacggaacgc tgatttttta tcttccgtca gcgtattcca
    11761 gtaagcccgc gccgcttcaa gcaatgtata agtcccgaca atgttggttt caataaacgc
    11821 tgccgggccg tcaatagaac ggtcaacatg gctttcggct gccagatgca tgacacagtc
    11881 tggctgatgc tcagtgaata cgcgtgccag ttctgcccga tcgcagatat caactttctc
    11941 aaaggcaaag tgctcgcttt gcgcgaccgg tgccagcgac atcaggtttc cggcgtaggt
    12001 cagcttatcg accactacca ccgcatcgct cgtttcgttg atgatataac gcaccagcgc
    12061 cgagccaata aacccggcac cacctgttat cagaattttt ctcatcagcg ccagactcct
    12121 ttggcatcga cgacatactg ctgatggaca ttgtcgccat tgataacttt gaactgacta
    12181 tgatcgacca gcatcaccag cacgtctgcc gttgccagcg cctcgtcaag ctgcgccaga
    12241 gtacaaagcc cggtcagttt tttcggcaac tggtggatgt taggctcaac aaccagcgtt
    12301 tcgccgctgt gccactgggc gatcagttca gcgatttcca tcgccgggct ttcgcgcagg
    12361 tcatcaatat tcggtttaaa cgccagacca aagcaggcga ttttcagttc actggcgcgt
    12421 ttatcggtag cagcaaggca gtcggcaacc gccgctttca cctgatcgat aacccagaac
    12481 ggtttgtgat cgttaacttc gcgcgcggta cggataagcc gcgcctgctg ggggttctgt
    12541 gccacgataa accacggatc aacagcaatg cagtgaccgc ccacgccagg gccaggctga
    12601 agaatattaa cgcgagggtg acgattcgcc aggcgaatca gttcccagac gttaatcccc
    12661 tgatcggcac aaatcagcga caattcatta gcaaaagcga tattcacatc gcggaagctg
    12721 ttttcggtga gcttacacat ttccgccgtc cgcgagttag tgacgacaca ctcaccttcg
    12781 aggaaaattt tgtacagttc gctggcgcgg gtcgaacaaa ccggcgtcat accaccaatc
    12841 acgcgatcgt ttttaatcag ctcgaccata acctggcccg gtaacacgcg ttccgggcag
    12901 taagcaatgt tgacgtccgc ctgctcgccc acctgctgtg ggaaagtgag atccggacgc
    12961 atctctgcta accattctgc catcttctcg gttgacccca ccggcgaggt ggattcaagg
    13021 atcaccagcg cgcctttttt cagcactggc gcaatggagc gagcagccga ttcaacgtag
    13081 gtcatatctg gctcatgatc gcctttaaac ggcgtgggta cagcaatcag ccaggcatcc
    13141 gcttcaactg gcgtcgtgct cgctcgtaaa aaaccgcctt ctacggcagt ttttactacg
    13201 ctcgccaaat caggctcgac gatatggatt tcgccacgat tgatggtatc aaccgcgtgt
    13261 tggttgatat cgacaccaat cacctgtttt tgccgtgagg caaacgctgc tgctgtaggc
    13321 agcccgatgt aacccagtcc gataacagaa atggtcgcaa aactcatagt gatatccgat
    13381 tattttttaa cgcttccaga atgcgagagc atgcctgacc atcaccatac gggttatggg
    13441 cgcggctcat agtttgatat tcgttttcgt cttttaaaag acgcgtcact tcctcgacaa
    13501 ttcgctgctt atccgtgcct accagacgca ccgtacccgc cgtcaccgct tccggacgct
    13561 cagtggtatc gcgcatcacc agcaccggtt tccccagcga aggcgcttct tcctgaatgc
    13621 cgcctgagtc ggtcaaaatc agccaggcgt ggttcatcag ccagacaaac ggtaaatact
    13681 cctggggatc gatcagaatg acatttttca catgccccag aatgcgattg accggctctc
    13741 tgacgttcgg gttgagatgc accggataga caatctggat gtcctggtgc gtggtggcga
    13801 tgtctgccag cgcgtggcag atttcttcaa agccacgacc gaaactttca cgcctgtgac
    13861 cggtcaccag aatcatcttt ttatcggggt cgataaacgg gtaatttgcc gccagttctg
    13921 aacgcagcgt gtcgctgctc atcacctgat cacgcaccca taacagtgca tcaatgactg
    13981 tattaccagt aatgaagatt cggctatccg caacgttttc acgcagcaag ttttgccggg
    14041 aagtttcggt tggagagaag tgatacatcg ccagatgccc ggtcaatgta cggttagcct
    14101 cttccggcca cggcgaatag agatcgcccg tgcgtaaacc agcctcaacg tgaccaacag
    14161 gaatacgctg ataaaacgcc gccaggctgg ttgccagcgt cgttgttgtg tcgccgtgaa
    14221 ccagcacgac gtctggtttg aactcggcaa gaataggttt tagcccttcc agaatccgac
    14281 aggttatctc tgtcaggccc tgtcctggct gcattatgtt gagatcgtag tcaggtacaa
    14341 tggaaaagag tttcagcacc tgatcgagca tctcccgatg ctgcgcagtg acgcaaactt
    14401 tagcctcaaa aaaaggatct tttgccaacg catgcaccaa cggagccatc ttgatggctt
    14461 ccgggcgcgt accaaataca gtcagtactt tcacatcgat tctcttcgaa taagcggcga
    14521 gcgcctttgc gctcgcagca gcagtgttgc tatttcgagc aacggcgggt taatgcgaca
    14581 ccagccccga tcagcccccc gacaatgccc cacataatca tcaggaaggc acgacgtggg
    14641 ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc gataggtctg aaaacgcgga
    14701 tccagagttg gaccaacatt cagggtgttt aacatggccc gattctgatc atagtcgaga
    14761 tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag cctggagcat tggacgccca
    14821 agcaggaaca tttctgaatc aggtaattcc tcggcaggca catctgtcgc actgcgcgaa
    14881 atattatgct gctcagcaat tttcagcgcc tgctcaatgc tgttcatccg gcggtcgtag
    14941 atggctttcg ccacctcttc ctgacgcttc acctgagctt tcatctggat ggtgcgtgcc
    15001 gcccatgcgc ctttcagctc atcattcaga tggctggctg cacgctggct ggcaaaagca
    15061 acatactgac gtaacaggtt attagcgtca ggcgcggttt cggcaataag cttcacgctg
    15121 tcattgaccg cgcgggtaaa gtctccgggg ataaactgga tgttgttaat catttcatcc
    15181 agcaacgccg catcggcttt gctgttgccc accatccgct gtttgtaata gtcggtttgc
    15241 agccagaact ctctgcgggt atcccacgag gccagttgca taacaaactc tttataggct
    15301 tcgtccatga ccgatggttg atcggcagaa gccatgtttg aacggacatc caggttacgc
    15361 aaaaattgct gctgcgagta atatcccccc agcatattca ccgttggacg atcggtaatc
    15421 gccgtcgagc tccactcctg acgagcaaaa aaagtatacg ccagcgcgat taacgcaaac
    15481 gccagcccca tgccaataat ccatagcttc ccagcccaca aggtacgaaa caacccacga
    15541 atatccagtt cattttcagc gtcttcggcc ggtttcccag gcattggttg tgtcatcaca
    15601 tcctcattta tttggttaaa ttggggctgc caccacgatt tctacgcagt ctgcgtttta
    15661 cgcgcttaat aaagcgagca actttccagg cacgcttaat gcaatatcca tagaggaaga
    15721 atgctagcaa aaagagcacc agcatgaccc actccgggac aaaatgagaa tattctgcca
    15781 gcacgccaat ggaagcgagc agtgctgcgg caagggtaat cagcacaaac gcctgacggg
    15841 aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg acggtcagga gagaatgggc
    15901 tcatgccttt acgcaggcga cggtacataa tcgccaccat atccattagc ggaatggcga
    15961 ttatccacaa agcggtaacc gggctgatgg gatgggtttt gccctgggtc gtttcgagca
    16021 ggatccagat aacggtaaaa ccaatcagcg tactgcccgc atcacccata aagactttgt
    16081 agcggcgacc caggatacca aggttaagca tgatgtatgg caggatggcg gcgatcatcg
    16141 caaagcacca gattgcgagg ctggtttgcc cgtcgaacca caaaatcata ccgattgctg
    16201 caaacgagac gcaggacaac ccgcccagca agccatcaat gccatcaacc atgttgaacg
    16261 cattaatggc cgcccagacg gcaaatagcg tcaggaagta accaaacggt ccgagcacca
    16321 tctcccagga gccaaagata taacccaggc tactgagata aagtttgccg aacaccatca
    16381 taacaatgcc aacagcggcc tgtatggtgg cacggatttt tacgctgata tcaaaacggt
    16441 catccagcgc gccaatgaaa acaagcacac cggcacaagc gagatagaga gatgcatgcg
    16501 gaatatagta atcgacaatt ccgaacgtga agcaaatccc tgcgtaaacc gaaatccccc
    16561 caacgagagg tatcaatccc tggtgacgtt tgcggaagtt tggtttatcc actaaaccga
    16621 ctttttttgc cactttacgg gcaaaaaaca gaaacagtgt cgtgaataaa aaaatactga
    16681 tgagatcagt actcactgtc agtaaattca caatgcatgc tctcagagaa aattattagc
    16741 agaagtataa ccacgaagac ctttattcag aagggaaagt ccgttccgaa cccaccagtc
    16801 ctgtgcaaaa attaaccatt tgatatgcaa attgcataac atgccagcaa attccaggaa
    16861 ttttcctaac atcgccagcg cggcattaag attacagata taaaagcaaa acgccacgta
    16921 aacacgtggc gtttttggca taagacaaat ttatgagcgt ttcatcattt cgaagaaatc
    16981 gtcattggtc ttagtcatgg ccagtttatt aatgaggaat tccattgcat cgatttcgcc
    17041 catcgggtga atgattttgc gcaggatcca cattttctgc agttcttcct gagtcgtgag
    17101 cagctcttct ttacgggtac cggaacggtt gtagtcgata gccgggaaga cgcgtttttc
    17161 agcgatctta cgagagaggt gcagttccat gttgcctgta cctttaaact cttcgtaaat
    17221 aacttcgtcc attttagaac cggtatcgat aagcgccgtc gcgataatgg tcaggctgcc
    17281 gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc ggacgatgaa gggcattggc
    17341 gtccacacca ccggtcaaca ctttacctga cgccggaaca acagtgttgt aagcgcgcgc
    17401 cagacgagtg atggagtcga gcagaatgat aacgtctttc ttgtgctcaa ccaggcgttt
    17461 cgccttctcg atcaccattt ccgcaacctg aacgtggcga gatgcgggtt cgtcaaaggt
    17521 agaagcaaca acttcacctt ttaccagacg ctgcatctcg gttacttctt ccggacgttc
    17581 gtcgatcagc agaaccatca gcacacaatc cgggtggttg taagcaatgc tctgagcaat
    17641 gttctgcagc agcatggttt taccggcttt cggcggtgcc acaatcagac cacgctgacc
    17701 acgaccgata ggtgatgcca gatccagtac gcgagcggtt aaatcttcag tagaaccgtt
    17761 accacgttcc atacgcagac gagagtttgc gtgcagcggg gttaagttct caaagaggat
    17821 tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg ttaactttca gcagcgcaaa
    17881 atagcgttca ccttctttcg gcgggcgaat cttaccagag atggtatcac cagtgcggag
    17941 gttgaaacgg cggatttggc taggggaaac gtagatgtca tcaggaccgg cgaggtagga
    18001 gctgtctgcg gaacggagga aaccaaatcc atcctgcaat atctccagta cgccatcacc
    18061 aaagatatct tcgccactct ttgcgtgctg cttcaggatg gcaaaaataa tgtcctgctt
    18121 acgcatacga gccaggtttt ccagccccat attttcgccg agagtgatca gctcagaaac
    18181 cggcgtattc tttaattcgg taagattcat agtggtgtga gttcttaaac ttggggtaaa
    18241 tctcgaactt aatgttgtga atggtatggc aggatcatcc atgcctgttt acggtcatcg
    18301 actcatgtct tttcgctgcc tggtcacagg agagtacgca gaactgaaac gacaagacgg
    18361 attgagtgac gagccagaaa tctgttcact tcgcatttaa gataaaacgg gaagcgttgg
    18421 aaattacaag attcaaactt aataaggtat gtttaatacg aagtcaacac taagttagca
    18481 tgactcacgc cgggcgtcca gtttttagcg acggggcacc cgaacatgaa attcccttac
    18541 gccaggttag cgtcgaggaa ctctttcaac tgacctttag acagtgcgcc cactttggtt
    18601 gccgccactt caccgttttt gaacagcagc agagtcggga taccacggat gccatatttc
    18661 ggcgcagtgc cagggttttg atcgatgttc agttttgcaa cggtcagttt gccctgatat
    18721 tcgtcagcga tttcatccag aatcggggcg atcattttgc acggaccgca ccactctgcc
    18781 cagaaatcga cgaggatcgc cccgtccgct ttgagtacat ccgtgtcaaa actgtcgtca
    18841 gtcaggtgaa taattttatc gctcatatat aactccacag gaataagcct ggcgtgttgg
    18901 tttcgttgtt ggtgtaacat taaccaacta aaggttgact ttatttcacc ggatacgctt
    18961 tcgtaaagca atagtaagct gatattctac cacactatga gcaaaacaca tttaacagaa
    19021 cagaagtttt ccgacttcgc cctgcatccg aaggttgtag aagcccttga aaaaaaaggg
    19081 tttcataact gtacgcccat tcaggcactg gcccttccgc tgacgctggc gggtcgtgac
    19141 gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg cgtttcttac gtcaacgttt
    19201 cattatcttc tctctcatcc tgcgattgcc gatcgcaagg tgaatcaacc acgtgcctta
    19261 attatggcac cgacgcgtga acttgccgtg cagatccatg ccgacgcaga accgctggcg
    19321 gaagctaccg gcctgaagct gggtctggct tacggtggtg atggctacga caaacagctg
    19381 aaagtgctgg aaagcggcgt tgacattctg attggcacta cggggcgttt aattgactac
    19441 gccaagcaga accacattaa cctcggtgcc attcaggtgg tggtactgga cgaagccgat
    19501 cgcatgtacg atctgggctt tattaaagat atccgctggc tgttccgccg tatgccgcct
    19561 gcaaaccagc gcctcaacat gctgttctcc gccacgcttt cgtaccgggt acgtgaactg
    19621 gcgttcgagc agatgaacaa tgccgaatat attgaagtgg aaccggaaca gaaaacgggc
    19681 caccgtatta aagaagagct tttctaccct tctaacgaag aaaaaatgcg tttgctgcaa
    19741 acgctgatcg aagaagagtg gccagacaga gcgattattt tcgccaatac caaacaccgt
    19801 tgtgaagaga tctggggcca cctggcggca gatggtcatc gtgtcggttt gttgacaggc
    19861 gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat ttacccgtgg cgatctggat
    19921 attctggttg ccaccgacgt tgccgcgcgt ggtttgcata ttccggcagt gacgcacgtc
    19981 tttaactacg atttacccga tgactgtgaa gattacgttc accgtattgg tcgtacaggt
    20041 cgcgcaggcg caagcggtca ctctatcagc ctggcgtgtg aagagtatgc attgaatttg
    20101 cctgctattg agacctatat tggtcactca attccggtaa gcaaatacaa tccggacgca
    20161 ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc cacgtacagg caatggcccg
    20221 cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt aaataaaatg tcatactcaa
    20281 aatcattcaa actgtatcaa gatggcaaga gaatgaatcc cgatgagcat actgtagtaa
    20341 gtgattcagg tgaaagaatg cagccaacac agagacagat tgaaggatga agagtatggg
    20401 ttccacctcg tcgctgtatg cagccattga tctcggttcg aatagttttc atatgctggt
    20461 tgtgcgcgag gtggctggaa gcatccagac gctgacgcga ataaaacgca aagtgcgtct
    20521 ggctgctggc ctgaatagcg aaaatgccct gtctaatgaa gcaatggagc gcggttggca
    20581 atgtctgcgc ctgtttgctg aacgtctgca agatatccct ccctcgcaaa ttcgcgttgt
    20641 cgctacggcg acattacgtc ttgccgtcaa tgcgggtgat tttattgcca aagcgcagga
    20701 aatcctcggt tgtccggtac aggtgatcag cggtgaagag gaagcacgtc tgatttatca
    20761 gggcgttgct cacacaactg gcggtgccga tcagcgcctg gtggtggata taggcggtgc
    20821 cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca gcctgtcgat
    20881 gggctgcgtc acctggctgg aacgctattt tgccgatcgt aatctggggc aggaaaattt
    20941 tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg aattacggta
    21001 tcacggctgg aaagtgtgcg ttggtgcttc cggcaccgtg caggcgttac aggaaatcat
    21061 gatggcacag gggatggatg aacgcattac cctggaaaag ttgcagcaat tgaaacagcg
    21121 agccattcat tgcggtcggc tggaggaact ggagattgac gggctgacgc tggaacgtgc
    21181 gttagtgttc ccgagtggtc tggcgatcct gatcgccatt tttaccgaac tgaatattca
    21241 gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggca tgttgcatct
    21301 ggccgtcgag caggatattc gcagccgtac gctgcgtaat attcagcgcc gctttatgat
    21361 cgatattgat caggcgcagc gcgtagccaa agttgcggct aacttcttcg atcaggtgga
    21421 aaaggaatgg catcttgaag caataagccg cgatttgctc atcagcgcct gtcagcttca
    21481 tgaaatcggc ctgagcgttg actttaaaca agcgccgcaa cacgctgctt atctggtgcg
    21541 taatctggat cttcccggtt ttacccccgc acagaaaaaa ttgctggcga cgctactgct
    21601 caaccagact aatccggtcg atctctcatc gctgcatcag caaaatgccg tacctccgcg
    21661 cgtcgcagaa caactctgcc gtttactccg cctggccatc atttttgcca gccgtcgccg
    21721 tgacgatctc gtgccagaga tgacattaca ggctaaccat gaactgttga ccttgacgct
    21781 tccgcaaggt tggctaaccc aacatccgct gggtaaagag attattgctc aggaaagcca
    21841 gtggcagagc tatgtccact ggccgctgga agtgcattaa tgagtaagtg ccggatgcga
    21901 tgctgacgca tcttttccgg ccttgattat ttccctcgtt ttgccgccat catcgctttc
    21961 agattcgcca gatggctttg ccctttctgc atccgttctt cggcgctgac cactttgcgc
    22021 tcctgttccc aaatcagatc atcctgcggc agctccagca aaaagcggct cggctccggg
    22081 cgcaccagtt cgccgtactg acggcgttct ttacacagcg taaaggtcaa ttccttctgg
    22141 gcgcgggtaa tgccgacata ggccagccgc cgctcctcat cgatattatc ttcatcgata
    22201 ctgctctggt gcggcaaaaa cccttcttcc ataccgacca tgtagacata aggaaactcc
    22261 agccctttcg acgcgtggag agtcatcagt tgcacctgat ccagctcttc ttcactctca
    22321 ccacgctcca tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag cgtcatcggc
    22381 tcatccagtt cgctaccttc cagcatctcc gtcatccagc taaacagttg gttgacgttc
    22441 ttcatgcgca tttcggcggc tttcgggctg ggcgatgttt cgtacagcca ggattcataa
    22501 tccatgccgt ggatcagatc gcgcaccgcg gcaatcggct cccgctccgc cagacgctgg
    22561 atttctgcca accagtgagt aaagcgggtc aatgcttcat aaccacgtcc gctaagcgtc
    22621 tgactcaggc ccatatcaaa gctggcggta aacatgcttt tattgcgcgt catcgcccac
    22681 tcacccagct ttttcagcgt cgccgggcca atctctctct tcggcgtgtt aacgatacgc
    22741 agaaatgcgc tgtcatcgtc cgggttagtc agcacgcgca gataagccag caagtccttg
    22801 atttcaggac gagagaaaaa cgacgtacca ccagatattt tgtatgggat gcggttttgc
    22861 atcaggaatt tttcaaacac ccgcgactga tggttaccgc gataaagaat ggcgtaatct
    22921 ttgtactgcg ttttattgac gaagtgatgg gcgatcagct cgccagtaac gcgctcggct
    22981 tcatgttcct cgttattcgc gcttaaaact tttagctctg tgccgtaacc cagttcggag
    23041 aacagacgct tttcaaagac gtgcggatta ttggcgatca ggatgttcgc cgctttcaaa
    23101 atacgtccgg aagagcggta gttctgctca agcttaatca ccttcagcgc cggaaaatcc
    23161 tggctcagca gcaccaggtt ttgcggacgt gcgccgcgcc aggagtagat cgactggtca
    23221 tcgtcaccca ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac cagttcatac
    23281 tggctggtgt tggtgtcctg atattcgtcc accagcaggt agcgaatttt gttctgccag
    23341 cgctcgcgga cttcttcatt gcgttgcaga agtagcgtcg gcaataaaat cagatcatcg
    23401 aagtcgagca cattacaggc tttcaggtgt gcatcataca gcccataaca atgggcaaaa
    23461 atacggtccc gctcgccaat cgcacttgct gccgcctggg acggtgtttt gagatcattc
    23521 ttccagttag agatggtcga aatcagttgt tgcaggagaa ctttgtcatc ttcaatcagc
    23581 ccctcggtca actctttaag caaagcaagc tgatcggtat cgtcaaacag cgaaaagttc
    23641 gccttcatcc caagcgccgc atactcgcgt ttgatgatat ccaaccccag cgtgtgaaaa
    23701 gtggagatca tcagcccgcg cgcctctttg cgacccagcg tctgcccgac acgctctttc
    23761 atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc ctgataaccg
    23821 caaccgcgga tcagatgggc gattttattg gtgatcacac gagttttacc ggaacccgcg
    23881 cccgccagca ccaggcaggg gccggtaacg aattcgacag cttgttgttg gccggggttt
    23941 agacgcatag gtgtattgct caatcttcga acggggggag gattgtagca gaatcgcatt
    24001 tgaatgtgac cgcgtgtaag ccgctaaccg gttggctgcg gatgtttaaa tcagagagag
    24061 aagaccgaaa ccggagttca tcgtgaatcc attagactta gaaaatatcg ggtcgtaacg
    24121 ttgtgttatt actttttcag agtttactaa ggagaggtgt actcgtggcg attaatttca
    24181 gtcctaaggt tggtgaaata ctggaatgca actttggtaa ctatcctgtt agccagaatg
    24241 gacagttcag caccaagtat tacgatggcc gcattcctcc tgaaatgata aaaaacaggt
    24301 tagttgtcgt gctcaacgga aaaattaatg gcaacgcatg tatcgtcgtt ccattatcaa
    24361 ccactcgcga tcatgacaag ttaaatcgtg gaatgcatgt tgaaatagcc agtaatgtta
    24421 tcaatggcct gcaattcttt gatcagcaga ttcgttgggc caaagccgac ctggtacaac
    24481 aagtcagtcg aaaccgatta aatagagcaa ggacttacag agggtatctt aatcaatgtt
    24541 tgccacatga gttggttgca gacatacaga gggcggtcat caagtcaatc aatgccatat
    24601 cgctcataaa ttgacaataa aatcaccatc cccgataatg actttgtagc ccatagaggc
    24661 cctaagtttt ctccccttga gggacctgcg aagcccggat tttccgggct tttgccatat
    24721 atgtaccagc ccctccctga ctgacaatta cgttagcatc tgcggatcat gctaaaatcg
    24781 ccgcctgaca attattcact caaggcacga tcatggcaaa aacagcagca gcactgcata
    24841 tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagattaag aacggtgccg
    24901 atttcggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc ggcggtgatt
    24961 taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagttgtg ttctcttgtc
    25021 cggtactgga gccaaccggc ccgctgcaca cccagttcgg ttaccacatc attaaggtgc
    25081 tgtaccgtaa ctaatagcaa ggccttctcc aggagaaggc cttgagtgtt ttctccctct
    25141 ccctgtggga gagggtcggg gtgagggcat cagcgcgcac ttaacccgca actgcaatac
    25201 gtttcatatc cgtcatatag ccgcgcagtt tcttacctac ctgctcaatc gcatggcagc
    25261 gaatcgcttc gttcacatca cgcagttgcg cgttatctac cgcaccttcc ggaatagctt
    25321 tacccagatc gcccggttgc agctctgcca taaacggttt cagcagcggc acacaagcgt
    25381 aagagaacag atagttaccg tactcggcgg tatcagagat aaccacgttc atttcgtaca
    25441 gacgcttacg ggcgatggtg ttggcaatca gcggcagttc gtgcagtgat tcatagtaag
    25501 cagattcttc gatgatgccg gaatcaacca tggtttcaaa cgccagctca acgcccgctt
    25561 tcaccatcgc gatcatcagt acgcctttat cgaagtactc ctgctcgccg attttgcctt
    25621 catactgcgg cgcggtttcg aaggcggttt tgccggtctc ttcacgccag gtcagcagtt
    25681 tcttatcatc gttggcccag tccgccatca taccggagga gaattcgccg gagatgatgt
    25741 cgtccatatg tttctggaac aggggtgcca tgatctcttt cagctgttca gacagcgcgt
    25801 aagcacgcag tttcgccggg ttagagagac ggtccatcat cagggtgatg ccgccctgct
    25861 tcagcgcttc ggtgatggtt tcccaaccga actgaattag tttttctgcg tatgccggat
    25921 cggtgccctc ttccaccagc ttgtcgaagc acagcagcga gccagcctgc aacataccgc
    25981 acaggatggt ttgctcaccc atcaggtcag atttcacttc cgcaacgaag gacgattcca
    26041 gcacgcccgc acggtgacca ccggttgctg ccgcccatgc tttagcgatc gccatacctt
    26101 cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt cggtacgccg aatccacgtt
    26161 tgtactcttc acgcacttcg gtgccagggc atttcggcgc aaccatgact acggtgatat
    26221 ctttacggat ctgctcgccc acttcgacga tgttgaaacc gtgcgagtaa cccagcgccg
    26281 cgccgtcttt catcagtggc tgtacggtgc gcactacatc agagtgctgc ttgtccggcg
    26341 tcaggttaac caccagatcc gcctgcggga tcagttcttc gtaagtacca actttaaaac
    26401 cgttttcggt cgctttacgc caggacgcgc gcttctcggc aatcgcttct ttacgcagag
    26461 cgtaggagat atcgagacca gaatcacgca tgttcaggcc ctggttcagc ccctgtgcgc
    26521 cacagccgac gataaccact tttttaccct gaaggtagct cgcgccgtcg gcgaattcgt
    26581 cgcgccccat aaagcgacat ttgcccagct gtgccagctg ctggcgcagg ttcagtgtat
    26641 tgaagtagtt agccatggtg attcctcgtg atgttgtgct tcttattgtt tggttcgcgt
    26701 ttgcgagatt gaattcacta tatgacagga aatttattgc ggaaattgat atattcacaa
    26761 cgtcacattg caatttctgc aacgtcaaca ttgagggctg cgccattgga tttacgcgat
    26821 ctgaaaacct tcctgcatct ggcggaaagc cgccattttg gccgcagcgc gcgggcgatg
    26881 cacgttagcc cgtccacgct ctcacggcag attcagcgcc tggaagacga tctcggtcag
    26941 gcattatttg tgcgcgataa ccggacggtg acgctgaccg aagcgggcga agagttacgc
    27001 gttttcgccc agcaaacatt gttgcagtat cagcaactgc gccacaccat cgatcagcaa
    27061 gggccgtcgc tctctggcga gttacatatt ttctgctcgg tgaccgccgc ttacagccat
    27121 ctaccgccga ttctcgaccg cttccgcgcg gaacacccgt cggtggagat taaacttact
    27181 actggtgatg cggcagatgc gatggaaaag gtggtcactg gtgaagcgga tctggcgatt
    27241 gcgggtaaac cggaaacctt gcccggcgca gtggcgtttt cgatgctgga gaatctggca
    27301 gtagtgctga ttgcccccgc gctgccctgc ccggtgcgta atcaggtgtc ggtagagaag
    27361 ccggactggt caacggtgcc gtttatcatg gccgatcagg ggccggtacg ccgccgcatt
    27421 gaactgtggt ttcggcgtaa caaaatcagt aacccgatga tttacgccac ggttggcggg
    27481 catgaagcga tggtatcgat ggtggcgctc ggctgtggcg tggcgctgtt gccggaagtg
    27541 gtgctggaaa acagcccgga accggtgcgt aaccgcgtga tgatactaga gcgaagcgat
    27601 gagaaaacgc cgtttgagct gggcgtgtgt gcgcagaaaa agcggctgca tgagccgctg
    27661 attgaagcgt tctggaagat tttaccgaac cactaacccg ccaaaaagaa cctgaacgcc
    27721 gggttattgg tttcgtcgtg gcaatcgtag cccagttcat tcagccgggt ttcgaaatcc
    27781 ggttcatggt cgccaagctc gaacgccgcc agtacgcgcc cgtagtcggt accgtgactg
    27841 cgataatgga acagagaaat attccagtgc gtacccagcg tgttgaggaa gcgcagcagc
    27901 gcacccggcg attccggaaa ttcgaagctg tagagacgtt cctgcaacgg atgcgatgga
    27961 cgcccgccaa ccatatagcg cacatgtagc ttcgccattt catcgtcgga gagatcaacc
    28021 acgctgtagc cgccgtcgtt gagcatctgc aaaatttctt tgcgctcttc gaggccgcgg
    28081 cttaagcgca cgccgacaaa gatgcaggcg tttttggcat cggcaaagcg gtagttgaat
    28141 tcggtgaccg aacgtccgcc aagcaactgg cagaatttga ggaagctacc tttctcttcc
    28201 ggaatggtta ccgccagcaa cgcttcacgc tgttcaccca gctcacaacg ttccgagacg
    28261 tagcgcaggc cgtgaaagtt cacgttagca ccggaaagaa tatgcgccag ccgttcgccg
    28321 cgaatgttgt gctgggcgat atattttttc attcccgcca gcgccagcgc gccagagggt
    28381 tccgccaccg cacgcacatc ttcgaataaa tccttcatcg ccgcacagat cgcatcgcta
    28441 tcgacggtga tgatgtcgtc gagatactcc tggcataaac ggaaggtttc gtcaccgatg
    28501 cgttttaccg ctacgccttc agcaaatagc cctacgcgcg gcagatcaac cggatgaccc
    28561 gcatccagcg ctgctttcag gcaggcggag tcttccgctt ctacggcgat cactttgatt
    28621 tgtggcatca gttgtttgat cagcaccgca acgcccgccg ccagaccgcc gccgccgact
    28681 ggcacaaata cgcggtcgag atgggcgtcc tgctggagca gttccagcgc cagcgtgcct
    28741 tgcccggcaa tcaccatcgg atggtcgaac ggcggcaccc aggtgaaccc ctgctgctgt
    28801 gacagttcga tcgctttagc tttcgcttcg tcaaagttcg cgccatgaag cagcacttcg
    28861 ccgccgaatc cacgtaccgc atcgactttg atgtcggcgg tggcggttgg cataacgatc
    28921 agggccttca cgcctaaccg cgcagaagaa aacgcgacgc cctgcgcgtg gttacccgca
    28981 gaagcagtga tcacgccgtg cgctttttgt tcttccgtca ggcccgccat catggcgtat
    29041 gcgccgcgca gcttaaagct gtgcactggc tggcgatctt cgcgcttcac cagaatgacg
    29101 ttatcaagac gcgacgacag tttttccatt ttttgtagcg gcgtaacctg cgccgcctcg
    29161 taaaccggcg cgcgcagcac tgctcttaaa tattcggcac cttccggagc accggacagg
    29221 ggttgcgagt cagccattat taacccccca gtttcgattt atcgcgcacc gcacctttgt
    29281 cggcgctggt tgccaggctg gcgtaggcgc gcagggcaaa ggaaacctga cgttcacggt
    29341 ttttcggcgt ccaggctttg tcaccccgag cttcctgcgc ttcacgacgc gccgccagtt
    29401 cggcatcgct tacctgtaac tgaatgccac ggttcgggat gtcgatagcg atcaggtcac
    29461 catcttcaat caggccaatg ctgccgccgt ttgccgcttc cggtgagacg tggccgatgg
    29521 aaagaccaga ggtgccgcca gagaaacgac cgtcggtgat cagcgcacag gctttaccga
    29581 gacccattga tttcaggaag ctggttgggt agagcatttc ctgcatgcca gggccgcctt
    29641 tcgggccttc atagcggatc accaccacat cgcccgcgac aactttgcca ccgaggatcg
    29701 cttctaccgc gtcgtcctgg ctttcgtaca ctttcgccgg gccggtgaat ttgaggatgc
    29761 tgtcatcgac gcccgcggtt ttaacgatgc agccgttttc tgcgaaatta ccgtagagca
    29821 ccgccaggcc gccgtctttg ctgtaggcgt gttccagcga gcggatacag ccattggcgc
    29881 gatcgtcatc gagagaatcc caacggcaat cctgcgagaa tgcctgtgtg gtacgaatgc
    29941 ctgcaggacc tgcgcggaac atatttttta ccgcgtcatc ctgggtcagc ataacgtcgt
    30001 attgttccag cgtttgcggc aacgtcaggc caagtacgtt tttcacatca cggttcagta
    30061 accccgcgcg atccagttcg ccgagaatac cgataacacc accagcacgg tgaacatctt
    30121 ccatatggta tttctgggtg ctcggcgcaa ctttacacag ctgtggaacc ttgcgggaaa
    30181 gcttatcgat atcactcatg gtgaagtcga tttccgcttc ctgcgccgcc gccagcaggt
    30241 gaagtacggt gttagtcgat ccacccatcg cgatatccag cgtcatggcg ttttcaaacg
    30301 ccgctttgct agcaatattg cgcggcagtg cactttcgtc gttttgctcg tagtaacgtt
    30361 tggtcaattc aacaatgcgt ttaccggcat tgaggaacaa ctgcttacgg tccgcgtggg
    30421 ttgccagcag cgagccgttg cccggctgcg acaggcccag cgcttcggtc aggcagttca
    30481 ttgagttagc ggtaaacatc ccggagcagg agccgcaggt cggacacgcg gaacgttcaa
    30541 cctgatcgct ctgggagtca gaaactttcg ggtctgcgcc ctggatcatc gcatcaacca
    30601 gatcgagttt gattatctga tcggacagtt tggttttccc ggcctccatc gggccgccgg
    30661 aaacaaagat caccggaata ttcaggcgca gggaagccat cagcatcccc ggggtgattt
    30721 tgtcgcagtt agagatgcag accatggcgt cggcgcagtg ggcgttgacc atatactcaa
    30781 cggaatcagc gatcagttcg cgagatggca gtgaataaag catccccccg tggcccatgg
    30841 caatcccatc atccaccgca atggtgttga actctttggc aacgccgcca gccgcttcaa
    30901 tttgttcggc gaccagttta ccgagatcgc gcagatggac gtgacccggt acaaattggg
    30961 tgaacgagtt cacaaccgcg ataatcggct tgccaaaatc ggcgtcggtc attccggtgg
    31021 cgcgccacag cgcacgagca cccgccatat tacgaccatg agtggtggtg gcggaacggt
    31081 acttaggcat actttattta ctcccagtgt ctgtctcgta aatgggacgg tgcgtgccgt
    31141 cccatttttt gtatttattg attaacttga tctaaccagc cccatttatc ttcggtttcg
    31201 ccagtgaaaa ggccgaagaa ggcttgctga atgcgtttgg taaccgggcc acaacggcct
    31261 tcgccaacct gaataccgtc tacgctgcgc actggcgtga tttctgccgc agtaccggac
    31321 ataaacactt catccgccag gtacagggat tcgcgcgaca gaacctgctc acgcacttca
    31381 attcccagct ctttcgccag tttgatgatg gcatcacggg taatacccgg cagcgcggag
    31441 gaggtgaacg gtggggtgaa cagcacacca tctttcactt caaacaggtt ttcgcctgcg
    31501 ccttcagaga tataaccgtt cacatccagc gcgatacctt cctgataacc gtggcggcgc
    31561 gcttcgctac ccaccagcag ggaagagaga tagttaccac cggcttttgc cgcggttggg
    31621 atggtgtttg gtgctgcgcg gttccaggag gaaaccatcg catcgatccc ctgctccagc
    31681 gcttctgcgc ccagatacgc tccccacggg aaagcggcga taatcacatc ggttgagtat
    31741 cccgctggcg ggtttacacc catgccaaca tcaccaacga agatcagcgg acggatatag
    31801 gcgctggtga gattgttttt gcggatcacg tcacgacaag cttccatcag ctcatcaatg
    31861 ctctgcgaaa ccgggaagcg atagattttg gcggagtcat gcagacgctg catatgctca
    31921 cgatggcgga atacaaccgg tcctttgtgc gagtcgtagc aacggatgcc ttcaaaaacc
    31981 gaagtgccat agtgcagcgc gtgcgacatc acatgcacct tcgcgtcttc ccagcgaacc
    32041 atctccccat tgaaccaaat gtaatcagct ttcttcgtgg tcatttttat attccttttg
    32101 cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg cagatggcaa cgtgtgcgac
    32161 gtccaccagt ttatttaact gactaaacag taagtcgacc gaccgtgggc tggcaacggt
    32221 caattcgata tttatatttt gtgcatcgct ggcggcagcc atattcattg agcagacgtg
    32281 gaaaccacga tgacgcacca cgcgtaaaac acgttctaag gtttccggat tgaagcgagc
    32341 cgatacattg acctgatgtt gcatcatgat aatttctcca acatttctga attactggcg
    32401 ccaggcggca ccagcggcca gacgttctca agttcgtcga ttgagacatg aagcaggtat
    32461 ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa cctggtcttt acgggtgatg
    32521 tgttggccag ggatgccgaa ggcgctggct aacatgagga aatcggggtt atcagtaagg
    32581 gtggtttcgc tgtatcgttc ctgaaaaaac agttgctgcc attgtcgaac catccctaac
    32641 cgttggttat cgagtaagac gattttcaac ggtaactgct tgcgttttac ggtgcccagc
    32701 tcttgcacat tcatcatgaa agagccgtca ccggagatac agacgacagt atcgttcggt
    32761 cgtgcgactt gtgcgccaac cgccgctggt aaaccgaaac ccatggtgcc taagccgctg
    32821 gaggtaatga aattttccgg gcgagtgtgt gcgatgtgct gcgcggccca catctggtgc
    32881 tgccccacat ctgtggtcac gacgcaatcc gcaggtttac gatccgacag ttgttttaac
    32941 aacaacggcg cgtagatagc gtcaccggga tggtcgtaac gccaggtatg ttcatcacgc
    33001 agctgcgcgc agtattgctg ccagtcattg atatttaacg gctgctgtaa tgctggtaac
    33061 agagcattta aatcaccttg taatgccaca tgtgcctgac gcagcttgtt catttctgcc
    33121 gggtcgatat ccatatggat aacactggcg tgtggtgcga aggtgttcag tttgccggtc
    33181 acccggtcat caaaacgtgc acccacggcg atcagcaagt cgcactcctg caccgcgaag
    33241 ttcgccgctt tggtgccatg cattcccagc atgcccaggt agtacggata atctgcttca
    33301 actgcgccca gccctttcag cgtgcaggtg gcaggcattt ttgtggtagc gagaaattct
    33361 cgtaaagcag gaactgcctg cgccataccc acgccaccac caacgtacag catcggtttt
    33421 tgcgcttttg ccagcatctg gcgcgcttgc tcaacttcgg catgggggaa agtcacttcg
    33481 ttttcaacgg tggtgaacca cggttccaga tcgccgctgg ctaactggat atcttttggg
    33541 atatcgacca gaaccggacc aggacgacct gagctggcaa cgtcgaatgc tgcagccatg
    33601 atgcgcggca actcttccag cgactgcacc agaaagctgt gcttggtaca ggctaacgac
    33661 aatcccagga catccacttc ctgaaatgcg tcggtgccga taaacggtgc ggacacttga
    33721 ccggtgatgg caacgacagg gatggaatct aacagtgcgt ccgcaagccc ggttatcagg
    33781 ttggttgcgc ccggaccaga cgtggcgata catacgccag ttttaccggt agcacgggca
    33841 taaccgatag ccgccattgc cgcaccctgc tcatgtcggc acagcaagtg ctccacgccg
    33901 ccgtcataca atgcatcgta aaccggcata attgcgccac ccggataacc gaaaacggtg
    33961 ttcacaccct gtgcccgcaa cgcatgtacc acccactgtg cgccattcat agttagttcc
    34021 ccgtcctgaa tcttgagaaa cagaattttg tgctgttatt cattgtctgc tcctcggtta
    34081 tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc gggggtctta gttcgttaag
    34141 gcttgatttc taagcctttc ctcgtccaag tgcagccccg cacggtggga taataatcac
    34201 caccacgcta atcacgacca ggctaatcac tcgtagaagg gctgtcattt tgtcttttct
    34261 tgcatcttgt tcgaaggaat gcctaaagag ttaccatagg ttttgtaaat agtacaagat
    34321 attttttggc cg
//
LOCUS       DAZFVO020000035        34244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFVO020000035 DAZFVO020000000
VERSION     DAZFVO020000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.035"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..147
                     /locus_tag="ACHKNB_RS07930"
                     /old_locus_tag="pgaptmp_001586"
     CDS             <1..147
                     /locus_tag="ACHKNB_RS07930"
                     /old_locus_tag="pgaptmp_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HGU4195122.1"
                     /translation="VLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAML
                     KQLS"
     gene            921..1676
                     /gene="repE"
                     /locus_tag="ACHKNB_RS07935"
                     /old_locus_tag="pgaptmp_001587"
     CDS             921..1676
                     /gene="repE"
                     /locus_tag="ACHKNB_RS07935"
                     /old_locus_tag="pgaptmp_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HGU4195123.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            2264..3430
                     /gene="sopA"
                     /locus_tag="ACHKNB_RS07940"
                     /old_locus_tag="pgaptmp_001588"
     CDS             2264..3430
                     /gene="sopA"
                     /locus_tag="ACHKNB_RS07940"
                     /old_locus_tag="pgaptmp_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HGU4195124.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3430..4401
                     /locus_tag="ACHKNB_RS07945"
                     /old_locus_tag="pgaptmp_001589"
     CDS             3430..4401
                     /locus_tag="ACHKNB_RS07945"
                     /old_locus_tag="pgaptmp_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HGU4195125.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            5268..6169
                     /locus_tag="ACHKNB_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /pseudo
     CDS             5268..6169
                     /locus_tag="ACHKNB_RS07950"
                     /old_locus_tag="pgaptmp_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(6120..6341)
                     /locus_tag="ACHKNB_RS07955"
                     /old_locus_tag="pgaptmp_001591"
     CDS             complement(6120..6341)
                     /locus_tag="ACHKNB_RS07955"
                     /old_locus_tag="pgaptmp_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HGU4195126.1"
                     /translation="MPAGSGKSARPVPGRTRDTDHRQRSCRAASAGAVKRGARRHPLP
                     EKKRAVARQSPSYFIHRNAPPCRLSTPAR"
     gene            6251..6478
                     /locus_tag="ACHKNB_RS07960"
                     /old_locus_tag="pgaptmp_001592"
     CDS             6251..6478
                     /locus_tag="ACHKNB_RS07960"
                     /old_locus_tag="pgaptmp_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195127.1"
                     /translation="MTRRGRNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDE
                     RPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            6555..7238
                     /locus_tag="ACHKNB_RS07965"
                     /old_locus_tag="pgaptmp_001593"
     CDS             6555..7238
                     /locus_tag="ACHKNB_RS07965"
                     /old_locus_tag="pgaptmp_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4195128.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            7239..7460
                     /locus_tag="ACHKNB_RS07970"
                     /old_locus_tag="pgaptmp_001594"
     CDS             7239..7460
                     /locus_tag="ACHKNB_RS07970"
                     /old_locus_tag="pgaptmp_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195129.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDACFND"
     gene            7354..7908
                     /locus_tag="ACHKNB_RS07975"
                     /old_locus_tag="pgaptmp_001595"
     CDS             7354..7908
                     /locus_tag="ACHKNB_RS07975"
                     /old_locus_tag="pgaptmp_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4195130.1"
                     /translation="MCLLNAWHARRCIAPIACSWRRIPKFWNWMRALTIEKETRMYGT
                     CETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLSDPEIRTVLARLEDIPEDQ
                     RIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDYGLAVPE
                     CVTRRQAVVNQARILLKNNTHENG"
     gene            7954..8730
                     /locus_tag="ACHKNB_RS07980"
                     /old_locus_tag="pgaptmp_001596"
     CDS             7954..8730
                     /locus_tag="ACHKNB_RS07980"
                     /old_locus_tag="pgaptmp_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195131.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRG"
     gene            9143..9568
                     /locus_tag="ACHKNB_RS07985"
                     /old_locus_tag="pgaptmp_001597"
     CDS             9143..9568
                     /locus_tag="ACHKNB_RS07985"
                     /old_locus_tag="pgaptmp_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4195132.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            9615..10037
                     /locus_tag="ACHKNB_RS07990"
                     /old_locus_tag="pgaptmp_001598"
     CDS             9615..10037
                     /locus_tag="ACHKNB_RS07990"
                     /old_locus_tag="pgaptmp_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4195133.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            10034..10225
                     /locus_tag="ACHKNB_RS07995"
                     /old_locus_tag="pgaptmp_001599"
     CDS             10034..10225
                     /locus_tag="ACHKNB_RS07995"
                     /old_locus_tag="pgaptmp_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195134.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(10195..10722)
                     /locus_tag="ACHKNB_RS08000"
                     /old_locus_tag="pgaptmp_001600"
     CDS             complement(10195..10722)
                     /locus_tag="ACHKNB_RS08000"
                     /old_locus_tag="pgaptmp_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195135.1"
                     /translation="MAPDTTTGRHPPQRREARFAKRRCPLHPGGLLPGCRERRPRRDR
                     GGATGTVAASQGFASGMEARQGGDSFAGSVHDSPPRQGDARKSHKQERNGKKRKKRKR
                     KKKKNSNRHGNHRKITSEKNIPDTSQTGRVTGDASAWPASLPAGVSLPGVFARADRRP
                     PPPALYHLFLLPCKM"
     gene            10792..10998
                     /locus_tag="ACHKNB_RS08005"
                     /old_locus_tag="pgaptmp_001601"
     CDS             10792..10998
                     /locus_tag="ACHKNB_RS08005"
                     /old_locus_tag="pgaptmp_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4195136.1"
                     /translation="MHIPQKFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            11023..11310
                     /locus_tag="ACHKNB_RS08010"
                     /old_locus_tag="pgaptmp_001602"
     CDS             11023..11310
                     /locus_tag="ACHKNB_RS08010"
                     /old_locus_tag="pgaptmp_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195137.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWNVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            11429..12250
                     /locus_tag="ACHKNB_RS08015"
                     /old_locus_tag="pgaptmp_001603"
     CDS             11429..12250
                     /locus_tag="ACHKNB_RS08015"
                     /old_locus_tag="pgaptmp_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4195138.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGNINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(12546..13148)
                     /locus_tag="ACHKNB_RS08020"
                     /old_locus_tag="pgaptmp_001604"
     CDS             complement(12546..13148)
                     /locus_tag="ACHKNB_RS08020"
                     /old_locus_tag="pgaptmp_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HGU4195139.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKNSKGIRIPATKKSLPEINSVQNN"
     gene            13471..13854
                     /gene="traM"
                     /locus_tag="ACHKNB_RS08025"
                     /old_locus_tag="pgaptmp_001605"
     CDS             13471..13854
                     /gene="traM"
                     /locus_tag="ACHKNB_RS08025"
                     /old_locus_tag="pgaptmp_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HGU4195140.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
     gene            14044..14730
                     /locus_tag="ACHKNB_RS08030"
                     /old_locus_tag="pgaptmp_001606"
     CDS             14044..14730
                     /locus_tag="ACHKNB_RS08030"
                     /old_locus_tag="pgaptmp_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HGU4195141.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSVSNTNTSGQLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
     gene            14824..15051
                     /gene="traY"
                     /locus_tag="ACHKNB_RS08035"
                     /old_locus_tag="pgaptmp_001607"
     CDS             14824..15051
                     /gene="traY"
                     /locus_tag="ACHKNB_RS08035"
                     /old_locus_tag="pgaptmp_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HGU4195142.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            15085..15450
                     /gene="traA"
                     /locus_tag="ACHKNB_RS08040"
                     /old_locus_tag="pgaptmp_001608"
     CDS             15085..15450
                     /gene="traA"
                     /locus_tag="ACHKNB_RS08040"
                     /old_locus_tag="pgaptmp_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HGU4195143.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            15465..15776
                     /gene="traL"
                     /locus_tag="ACHKNB_RS08045"
                     /old_locus_tag="pgaptmp_001609"
     CDS             15465..15776
                     /gene="traL"
                     /locus_tag="ACHKNB_RS08045"
                     /old_locus_tag="pgaptmp_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HGU4195144.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            15798..16364
                     /gene="traE"
                     /locus_tag="ACHKNB_RS08050"
                     /old_locus_tag="pgaptmp_001610"
     CDS             15798..16364
                     /gene="traE"
                     /locus_tag="ACHKNB_RS08050"
                     /old_locus_tag="pgaptmp_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HGU4195145.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLRYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            16351..16599
                     /locus_tag="ACHKNB_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /pseudo
     CDS             16351..16599
                     /locus_tag="ACHKNB_RS08055"
                     /old_locus_tag="pgaptmp_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
     gene            16597..17565
                     /locus_tag="ACHKNB_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /pseudo
     CDS             16597..17565
                     /locus_tag="ACHKNB_RS08060"
                     /old_locus_tag="pgaptmp_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            17562..18071
                     /gene="traS"
                     /locus_tag="ACHKNB_RS08065"
                     /old_locus_tag="pgaptmp_001613"
     CDS             17562..18071
                     /gene="traS"
                     /locus_tag="ACHKNB_RS08065"
                     /old_locus_tag="pgaptmp_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HGU4195146.1"
                     /translation="MITHYDIKMETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPEKFRDESKVISF
                     MYDKNKNYILSFLIAFSMVSFAHTLLYEFLLIALFIIFFFIYAIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            18085..18816
                     /gene="traT"
                     /locus_tag="ACHKNB_RS08070"
                     /old_locus_tag="pgaptmp_001614"
     CDS             18085..18816
                     /gene="traT"
                     /locus_tag="ACHKNB_RS08070"
                     /old_locus_tag="pgaptmp_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HGU4195147.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKTTVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSSAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            19068..21221
                     /gene="traD"
                     /locus_tag="ACHKNB_RS08075"
                     /old_locus_tag="pgaptmp_001615"
     CDS             19068..21221
                     /gene="traD"
                     /locus_tag="ACHKNB_RS08075"
                     /old_locus_tag="pgaptmp_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HGU4195148.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(21230..21628)
                     /locus_tag="ACHKNB_RS08080"
                     /old_locus_tag="pgaptmp_001616"
     CDS             complement(21230..21628)
                     /locus_tag="ACHKNB_RS08080"
                     /old_locus_tag="pgaptmp_001616"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4195149.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(21628..21855)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS08085"
                     /old_locus_tag="pgaptmp_001617"
     CDS             complement(21628..21855)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS08085"
                     /old_locus_tag="pgaptmp_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4195150.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            21937..27207
                     /gene="traI"
                     /locus_tag="ACHKNB_RS08090"
                     /old_locus_tag="pgaptmp_001618"
     CDS             21937..27207
                     /gene="traI"
                     /locus_tag="ACHKNB_RS08090"
                     /old_locus_tag="pgaptmp_001618"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HGU4195151.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTQFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            27227..27973
                     /gene="traX"
                     /locus_tag="ACHKNB_RS08095"
                     /old_locus_tag="pgaptmp_001619"
     CDS             27227..27973
                     /gene="traX"
                     /locus_tag="ACHKNB_RS08095"
                     /old_locus_tag="pgaptmp_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HGU4195152.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            28032..28892
                     /locus_tag="ACHKNB_RS08100"
                     /old_locus_tag="pgaptmp_001620"
     CDS             28032..28892
                     /locus_tag="ACHKNB_RS08100"
                     /old_locus_tag="pgaptmp_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4195153.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            28995..29552
                     /locus_tag="ACHKNB_RS08105"
                     /old_locus_tag="pgaptmp_001621"
     CDS             28995..29552
                     /locus_tag="ACHKNB_RS08105"
                     /old_locus_tag="pgaptmp_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HGU4195154.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            29706..29909
                     /locus_tag="ACHKNB_RS08110"
                     /old_locus_tag="pgaptmp_001622"
     CDS             29706..29909
                     /locus_tag="ACHKNB_RS08110"
                     /old_locus_tag="pgaptmp_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195155.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            30651..31112
                     /locus_tag="ACHKNB_RS08115"
                     /old_locus_tag="pgaptmp_001623"
     CDS             30651..31112
                     /locus_tag="ACHKNB_RS08115"
                     /old_locus_tag="pgaptmp_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HGU4195156.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            31200..31598
                     /locus_tag="ACHKNB_RS08120"
                     /old_locus_tag="pgaptmp_001624"
     CDS             31200..31598
                     /locus_tag="ACHKNB_RS08120"
                     /old_locus_tag="pgaptmp_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195157.1"
                     /translation="MKGEQINYQAVSELITSSNHNVLIVGGSASEVDSFLNKLNITDY
                     KYYDFSQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVA
                     RKSGKQLIVAVREQDMKKMEAEFGRIIKIH"
     gene            31878..32027
                     /locus_tag="ACHKNB_RS08125"
                     /old_locus_tag="pgaptmp_001625"
     CDS             31878..32027
                     /locus_tag="ACHKNB_RS08125"
                     /old_locus_tag="pgaptmp_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4195158.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            32311..32571
                     /locus_tag="ACHKNB_RS08130"
                     /old_locus_tag="pgaptmp_001626"
     CDS             32311..32571
                     /locus_tag="ACHKNB_RS08130"
                     /old_locus_tag="pgaptmp_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HGU4195159.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            32795..32869
                     /gene="tap"
                     /locus_tag="ACHKNB_RS08135"
                     /old_locus_tag="pgaptmp_001627"
     CDS             32795..32869
                     /gene="tap"
                     /locus_tag="ACHKNB_RS08135"
                     /old_locus_tag="pgaptmp_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HGU4195160.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            32862..33719
                     /gene="repA"
                     /locus_tag="ACHKNB_RS08140"
                     /old_locus_tag="pgaptmp_001628"
     CDS             32862..33719
                     /gene="repA"
                     /locus_tag="ACHKNB_RS08140"
                     /old_locus_tag="pgaptmp_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4195161.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRAQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
BASE COUNT         8394 a         7997 c         9438 g         8415 t
ORIGIN      
        1 gttctgcaaa gcctgatggg acataagtcc atcagttcaa cggaagtcta cacgaaggtt
       61 tttgcgctgg atgtggctgc ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg
      121 gttgcgatgc tgaaacaatt atcctgagaa taaatgcctt ggcctttata tggaaatgtg
      181 gaactgagtg gatatgctgt ttttgtctgt taaacagaga agctggctgt tatccactga
      241 gaagcgaacg aaacagtcgg gaaaatctcc cattatcgta gagatccgca ttattaatct
      301 caggagcctg tgtagcgttt ataggaagta gtgttctgtc atgatgcctg caagcggtaa
      361 cgaaaacgat ttgaatatgc cttcaggaac aatagaaatc ttcgtgcggt gttacgttga
      421 agtggagcgg attatgtcag caatggacag aacaacctaa tgaacacaga accatgatgt
      481 ggtctgtcct tttacagcca gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg
      541 agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca cgatgcctga
      601 aaaaacttcc cttggggtta tccacttatc cacggggata tttttataat tatttttttt
      661 atagttttta gatcttcttt tttagagcgc cttgtaggcc tttatccatg ctggttctag
      721 agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat taccctcaaa tgacagtcct
      781 gtctgtgaca aattgccctt aaccctgtga cagattgccc tcagaagaag ctgttttttc
      841 acaaagttat ccctgcttat tgactctttt ttatttagtg tgacaatcta aaaacttgtc
      901 acacttcaca tggatctgtc atggcggaaa cagcggttat caatcacaag aaacgtaaaa
      961 atagcccgcg aatcgtccag tcaaacgacc tcactgaggc ggcatatagt ctctcccggg
     1021 atcaaaaacg tatgctgtat ctgttcgttg accagatcag aaaatctgat ggcaccctac
     1081 aggaacatga cggtatctgc gagatccatg ttgctaaata tgctgaaata ttcggattga
     1141 cctctgcgga agccagtaag gatatacggc aggcattgaa gagtttcgcg gggaaggaag
     1201 tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa aggctatgaa tcttttccct
     1261 ggtttatcaa acgtgcgcac agtccatcca gagggcttta cagtgtacat atcaacccat
     1321 atctcattcc cttctttatc gggttacaga accggtttac gcagtttcgg cttagtgaaa
     1381 caaaagaaat caccaatccg tatgccatgc gtttatacga atccctgtgt cagtatcgta
     1441 agccggatgg ctcaggcatc gtctctctga aaatcgactg gatcatagag cgttaccagc
     1501 tgcctcaaag ttaccagcgt atgcctgact tccgccgccg cttcctgcag gtctgtgtta
     1561 atgagatcaa cagcagaact ccaatgcgcc tctcatacat tgagaaaaag aaaggccgcc
     1621 agacgactca tatcgtattt tccttccgcg atatcacttc catgacgaca ggatagtctg
     1681 agggttatct gtcacagatt tgagggtggt tcgtcacatt tgttctgacc tactgagggt
     1741 aatttgtcac agttttgctg tttccttcag cctgcatgga ttttctcata ctttttgaac
     1801 tgtaattttt aaggaagcca aatttgaggg cagtttgtca cagttgattt ccttctcttt
     1861 cccttcgtca tgtgacctga tatcgggggt tagttcgtca tcattgatga gggttgatta
     1921 tcacagttta ttactctgaa ttggctatcc gcgtgtgtac ctctacctgg agtttttccc
     1981 acggtggata tttcttcttg cgctgagcgt aagagctatc tgacagaaca gttcttcttt
     2041 gcttcctcgc cagttcgctc gctatgctcg gttacacggc tgcggcgagc gctagtgata
     2101 ataagtgact gaggtatgtg ctcttcttat ctccttttgt agtgttgctc ttattttaaa
     2161 caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt aaattgcaag
     2221 atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac tcatggaaac
     2281 acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca ttgcacagtt
     2341 taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag gtgaagcagc
     2401 ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag ggcgactacc
     2461 gcacccggat atggaaattc gaggacgggt tgagcaacgt gttggttata caattgaaca
     2521 aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag acgtatttcc
     2581 accggtgatc ggggttgctg cccataaagg tggcgtttac aaaacctcag tttctgttca
     2641 tcttgctcag gatctggctc tgaaggggct acgtgttttg ctcgtggaag gtaacgaccc
     2701 ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc atgcagaaga
     2761 cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa taaagcccac
     2821 ttgctggcct gggcttgaca ttattccttc ctgtctggct ctgcaccgta ttgaaactga
     2881 gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga tgctccgact
     2941 ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg cgcctaacct
     3001 gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc ccacgcctgc
     3061 tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg atctgctcaa
     3121 gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca aatacagcaa
     3181 tagtaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct ggggaagcat
     3241 ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga tccggatgag
     3301 aactgttttt gaacaggcca ttgatcaacg ctcttcaact ggtgcctgga gaaatgctct
     3361 ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac cacgctggga
     3421 gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac tcaaccggtt
     3481 ggagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat tgcgcgcgta
     3541 ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga tgtgaagttt
     3601 actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg gtcaggtaat
     3661 gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc ttcttttcta
     3721 ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat agaaattgcc
     3781 gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg tgttctggtt
     3841 ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga ttatcgccca
     3901 acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga atttgctgga
     3961 aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac ccgctgtatc
     4021 aacacagcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg tgaactatct
     4081 gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt acttaagcag
     4141 caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc tgaagaagtt
     4201 atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag tttaagctca
     4261 cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat ggtgcttaac
     4321 ctggacaggt ctcgtgttcc aactgagtgt atagagaaaa ttgaggccat tcttaaggaa
     4381 cttgaaaagc cagcaccctg atgcgaccac gttttagtct acgtttatct gtctttactt
     4441 aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc tgggcccact
     4501 gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac tcgtatcgtc
     4561 ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct
     4621 gggaccatgg tcccactcgt atcgtcggtc tgattattag tctgggacca tggtcccact
     4681 cgtatcgtcg gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat
     4741 tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac
     4801 gatcccactc gtgttgtcgg tctgattatc ggtctgggac cacggtccca cttgtattgt
     4861 cgatcagact atcagcgtga gactacgatt ccatcaatgc ctgtcaaggg caagtattga
     4921 catgtcgtcg taacctgtag aacggagtaa cctcggtgtg cggttgtatg cctgctgtgg
     4981 attgctgctg tgtcctgctt atccacaaca ttttgcgcac ggttatgtgg acaaaatacc
     5041 tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag tgtgtcgctg
     5101 tcgccggcct cctcacccgg tcacgtttcg tcgttcctcc tccacgcgct gcggcttcgg
     5161 ggccgcacct gcattcgtat gcggtcgccc ggttacaggt gcggcacggc ctgatggagg
     5221 ccgcatgtga gaggagaatt cccatgccaa actggtgctc gaatcgtatg tatttttctg
     5281 gtgaaccggc acagatcgct gagattaaac gactggccag cggtgcagtc actccgcttt
     5341 atcgccgcgc cacaaatgaa ggtattcagc tgtttctggc cggaagtgcc ggacttctgc
     5401 agaccactga agatgtgcgg tttgaaccat gccccggact gacggctgcc gggcgtggcg
     5461 ttgtatcgcc ggagaatatc gcgttcaccc gctggctgac acacctgcag gacggtgtac
     5521 ttctggatga gcgaaactgc ctgatgctgc atgaactctg gctgcagagc ggtactggcc
     5581 ggcgtcgctg ggaagaatta ccggatgatg ccagggaaag cattaccgct cttttcaccc
     5641 caaaaagagg tgactggtgc gacatctgga gtaacgagga tgtatcggtg tggtggaacc
     5701 gtctgtgtga caacgtcctg ccggaaaaac catgccgttt gacctgctgt cggttctgtc
     5761 cactcgcctg gatgttgaag tgaatggctt taacggtggt gttctgaacg gtgttccttc
     5821 tgcatatcac tggtatacgg aacagtatgg cgtgaagtgg ccttgtgggt atgaggtgaa
     5881 tatcagcagt cagggagaca acttcattca ggttgatttc gacacgccgt ggtgtcagcc
     5941 ggaaagcgac gttattgcag tattaagccg ccgtttcagt tgcacgctgg agcactggta
     6001 cgccgagcag ggctgcaatt tctgtggctg gcagcgatat gagcgtggag agcttgttga
     6061 tgtgctgtgg ggggaactgg aatggtcttc cccgacagat gatgatgagc tgccggaagt
     6121 caccgcgccg gagtggatag tcgacaaggt ggcgcattac ggtggatgaa gtatgacgga
     6181 gactggcggg caaccgcccg tttcttttcc ggtaaaggat gtcgccgggc tcctcttttt
     6241 actgcgccgg ctgacgcggc gcggcaggaa cgctgcctgt ggtcagtgtc ccgcgtccgg
     6301 ccgggcacgg gacgggcgct tttaccgctc ccggctggca tcggtgacag tgtacgccag
     6361 cccgtcgccc ttttctgatg aacgcccttc aagccgcttt cgcggcatat tctcgccgtc
     6421 aaaaagacga cggctgcgat attccacggt cggcctgacc cgttaccgga cgcggtgaac
     6481 agcccacagg ccgcggggaa cgggcgccac aggggcgccc tcccggtgcc ctctgtaaga
     6541 gaaggagtgc tgttatgtcc cgttttatcc agggtgactg cgtgcgtgtt atggccacgt
     6601 ttccgggcaa tgctgtcgat ttcatcctca ccgacccgcc atatctcgtc ggtttccgtg
     6661 accgtcaggg gcgtaccatc gccggcgata aaaccgatga atggctgcaa ccggcctgta
     6721 atgaaatgta ccgcgtgctg aaaaaagatg cgctgatggt gagcttctat ggctggaacc
     6781 gcgtcgatcg ctttatggcc gcctggaaaa atgcgggatt cagcgtggtt ggccacctgg
     6841 tgtttaccaa aacctacaca tcgaaggccg catatgtggg ctatcgccac gaatgcgctt
     6901 acatcctggc aaaagggcgt ccggcgctgc ctcagaagcc attgcccgac gtgctgggct
     6961 ggaaatattc aggcaatcgt catcacccga cggaaaagcc tgttaccagc ctgcaaccac
     7021 tgattgagag cttcacacac ccgaacgcga ttgtgctgga cccgtttgca ggcagcggct
     7081 caacctgtgt ggccgccctt cagtccggac gccggtatat cggtatcgag ctgcttgaac
     7141 agtatcaccg tgccggacag caacgtctgg ctgccgtaca gcgggccatg cagcaggggg
     7201 ccgcaaatga tgactggttt atgccggagg ctgcgtaaat gaactatgcc ggacatgaaa
     7261 aacttcgcgc cgacgtggcg gaggtggcca acaccatgtg tgaccttcgg gccaggctga
     7321 acgatatgga gcatcgctgt cgctttgatt ccgatgtgct tgttgaacgc ttggcacgcc
     7381 agacgctgta tcgcgccaat cgcctgttca tggaggcgta taccgaaatt ctggaactgg
     7441 atgcgtgctt taacgattga gaaggagacg agaatgtacg gaacatgcga aacgctatgc
     7501 cgggagctgg cagcaaagta tccgggatat acaccgctga tgctggttat ctggtccccg
     7561 gaagagattc aggccctcgc tgacggaatg gatatttccc tgtccgatcc tgaaatcagg
     7621 accgtcctgg cgcgcctgga ggacatcccg gaagaccagc gaattgagtc cggtatttct
     7681 tctgccgcgg cgatggagat tatcagcaac gtgagcgaaa accgccaggt gaccgtcccg
     7741 gctgaactgc tggcgtccct gattcagacc gccgaacagg cactgtggaa acgtgaatgg
     7801 gccgcccgtg attacggcct cgccgtcccg gaatgcgtta cccgccgtca ggcggtggtt
     7861 aatcaggccc gcatcctgct gaaaaacaac acacacgaaa acggctgatg ttatcgccgc
     7921 ctgcgggcgg cgatacaggg agaacgatta atcatgaacg aaaacacaac gctgaacgca
     7981 ctgatttgtc gtcacgcccg taacctgctg ctggcgcagg gctggccgga agagacggat
     8041 gtcgaccagc gtaacccgaa gtatccgggc tggatcagca tttatgttct gctggatgcg
     8101 ccccggctgg cgacgttact cgttaaccgc cacggcggcg tactgccgcc gcacctggcc
     8161 tccgccattc agaaactgac cggaaccgga gcggaacttg ttctgtccgg cagtcagtgg
     8221 cagtcgctgc cggtgcttcc ggcagacgga acgcaggtgt cttttccgta tgccggtgaa
     8281 tggctgaccg aagacgaaat cagggctgta cttgcggcgg tgcgcgatgc ggtacgcagt
     8341 gtcagttgcc gggtggcaga agatacgcgg cgtatccggg cggcgctgac caccaccggt
     8401 cagacgttac tgacccgcca gacgcgccgc tttcgcctgg tcgtgaagga aagcgatcac
     8461 ccctgctggc tcgatgagga cgacgaaaac ctgcccgtgg tgctcgatgc catcctgaac
     8521 cggggcgcac gtttttcggc ggtggaaatg tatctggtca gcgattgtat tgaacatatc
     8581 ctgtccagtg ggctggcctg cgatgtgctg cgtataccgg atgaaccgcc ccgccagtgg
     8641 tttgaccgtg gtgttctgcg ggaagttgtc cgggaagccc gggccgaaat ccgcagcatg
     8701 gcggatgccc tggcaaaaat caggggctga tgatggcggg gaaaatcccc gctttttttc
     8761 tgccggtccc cggacagagc acaaaggaag tcgccgccgg ctccgcttta tccggccatg
     8821 caggcatggc cttgtgggtt ttcagttatg tggccgccgt gtcgtgtgcg ggcggtgccg
     8881 tgcctccatc ttagccgggc tggcggggat gcaagggtac gcttcgccgc tgcggtcacc
     8941 cggtccttcc ttcccgtccg ccgtgatttt ccggtcgttc acccgctcag gtttcgcggt
     9001 ctcaccctcc aactcccgcc agccgtgtgc gtcaggctgc ggcttccttt gcgccctgtg
     9061 catcccgcct tatcgcccgg cttttatgga ggcacggcac cgcccggtgt cgcggaatat
     9121 gtgaataacg gaggaaaaca tcatgcaata tgcgaaacct gtcactctga acgttgaaga
     9181 gtgcgaccgt ctctcctttc tgccttacct gtttggcaat gattttctgt atgccgaggc
     9241 gtatgtgtac gcgctggcgc aaaaaatgat gccggaatat cagggaggct tctggcactt
     9301 catccgcctg ccggacggtg gcggttacat gatgccggat ggcgaccgtt ttcatctggt
     9361 caacggtgaa aactggtttg accgcacggt cagcgccgat gccgcaggca tcattctgac
     9421 ttcccttgtg attaaccgcc agttgtggct gtaccacgac agcggggatg caggactgac
     9481 ccagctttac cggatgcgcg atgcgcagtt gtggcgtcac atcgaatttc accccgaatg
     9541 caacgcgatt tacgcggcac tggactgatt aacggacggg gcggcaatgc cgcccctgta
     9601 aaggagacga gaacatgtac tgtactgtta aagaaatcat ccgcgatgta ctggatacag
     9661 acgtgccgga cagtgaatgc gtttttgccg tggtgctgac ccgtggggat gtgcgccaca
     9721 tagcccagga ctggaacctg tcagacgatg agctggaaac cgtcatgcag cggctggacg
     9781 atgcctttga atatggtgcg gatgtcagca ttgttcacga cgttgttcgt gagctgatgg
     9841 aagaaaagcg tgccagccgt caggtgacag ttccggcggt gatgctggaa aaagtgatgg
     9901 cgctggcagg cagtgaaatg aagcgcctgt atgccgtcgg gagtgagaac gggggcgacg
     9961 gtgatgcgtt cgtcagggag gaacgcgaag caatggacgt tgtgttacag gcgctggacg
    10021 gggagcacat gtcatgaata tcagcacaga aacccgcgaa attctgcgca attacaaagc
    10081 cgtgattaat gcgcgacgtc gtgaaatggg gcagaaaccg ctcaccaccg cgcagattgt
    10141 tgatgaaatc tgcgattttg tggcgaatca gcaggctgtt ttcctgggcg gtcactacat
    10201 cttacagggc agcagaaaca ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg
    10261 cgcgaaaacc cctggcaggc tgacgcctgc cggaagggaa gcgggccacg ctgacgcgtc
    10321 gcccgttacc cttcctgttt gtgatgtgtc cggaatgttt ttttctgatg ttatttttct
    10381 gtggttaccg tgcctgttac tgttcttctt ctttttcctc ttcctcttct tcctcttctt
    10441 tccgttcctc tcctgcttat ggcttttccg ggcgtccccc tggcggggcg ggctgtcgtg
    10501 aacggagcca gcgaagctgt ctccgccctg gcgggcttcc atccctgacg cgaaaccctg
    10561 gcttgcagcg accgttccgg tcgcgccgcc tctgtcgcgc cgtggcctgc gctcccggca
    10621 tcccggcaaa aggccgccgg gatgtaaggg gcaacgccgt ttcgcaaacc gcgcttcacg
    10681 tcgttgcggc gggtgcctgc ccgttgttgt gtccggtgcc attcacctgt ttaagcccct
    10741 gtagccgggg gctgatacag ccacaaccgt ctccgccagt ccaggggtga aatgcacatc
    10801 ccgcaaaagt ttttgcccaa aagcaggcaa aaacttttgc ggtctgcccc cggacagtcg
    10861 tttccggccg tggcagtgat gccccttgcg gcgaaggttc tcaaaaaggt gatggtgaat
    10921 accggaggga acaaaaggca ggtccccgca aaacgtcaaa accgcggctc ccgaaactca
    10981 aaagatgatg ggggctgaaa accagagaga ggagaaagca ggatgagtac ccgaaatatc
    11041 cacgttaaca ccgcgtcgta cacgcttctt gttgccggga agaaaaaaaa caccggggaa
    11101 gagtggaacg tcctggaatt cagcagcctg actgagctga aaaaatatcg caaaagccac
    11161 ccggaaaaga tggctttcag ctatagctac gcgctcagcc ggggtgtgga tacgcagttc
    11221 cgccatatca acattgcgga agccgatcac ttcaaacagt tcctgcgtca gattaagcgt
    11281 gccggcctcg atatccgggc gatctgctga ccggatgtga tgaatatccc tcctgattgt
    11341 tgcaatcttg ttaatggtga ccagggggga tgatattaag cggaaaaatt ttttattaaa
    11401 tttattcttt cataaaggag tagctgttat gcgattagct tcccgttttg gtcggtataa
    11461 ttccatccgc cgtgaacgtc ctttaacgga tgatgaatta atgcagttcg tgccttcggt
    11521 attttccggt gataaacatg agtcccggag tgaacgttat acgtatattc caacaatcaa
    11581 tatcatcaat aagttacgtg atgaaggttt ccagccattc tttgcctgtc agagtcgggt
    11641 tcgtgatttg ggacgtcgcg aatacagtaa acatatgtta cgtcttcgca gggaagggaa
    11701 tattaacggg caggaagttc ctgaaattat cttgcttaat tcacatgatg gttcatccag
    11761 ttatcagatg atccccggaa tttttcgttt tgtctgcaca aatggcctgg tgtgcggaaa
    11821 taattttggc gaaatccgcg ttccacataa aggtgatatt gtcgggcagg ttatagaggg
    11881 cgcgtatgaa gtgctcggtg tctttgataa ggtcactgat aatatggagg cgatgaaaga
    11941 aattcatctt aacagtgacg agcaacattt atttggcaga gctgcactga tggtcaggta
    12001 tgaagatgaa aataaaacgc cagtgacgcc tgaacaaata attactcccc gtcgttggga
    12061 ggataaacag aacgatctct ggacaacctg gcagcgggtt caggagaata tgataaaagg
    12121 tggattatcg gggcgaagtg cctccgggaa aaataccagg acaagagcca ttacaggtat
    12181 tgatggtgat ataagaatca acaaggcgtt gtgggtgatt gccgaacagt tcagaaaatg
    12241 gaagtcatga taatataagt aaagccccga aaaaatttcg gggctttact tatattatca
    12301 ttcttcggca tcgctgtgtc gtctgatata cctcaatttc atcaactggc ttttcggttg
    12361 tctgtaaata ttgtgcccgc gaaaaatggt ctggcaggag gctgcgcagt taccgggtat
    12421 atataagatg ggttaccgct tcgcggggct tcggccagtc agtattgtca ctcatatgat
    12481 atttttgtgt gtggccttcc atgccgcttt tgcggcataa ccggtatctg acaatgtctg
    12541 taagattaat tgttctgcac gctgttaatt tccggaagtg atttcttcgt ggccggaatc
    12601 cggatacctt tactgttttt tatcccggta taaatccggt aaacatctga tgcgtaggca
    12661 agacgtctct ggttctggcg ttcggtcttc ctgaatccag cattgtatgc accaacggcc
    12721 tcccaggaga cgccccattt tttaaaggct attgccagat aataagcacc ggtataaatg
    12781 ttcatgcagg gatctgttgt cagatgttcc ggcttaattc catagcgggc cagttcgtta
    12841 aaatgctggg aatctacctg catcagtccg ctgccatatc ccgttaccgg attaataccg
    12901 atggcattaa cccggtaacg ggattctttc catgatattg ctctcagtaa atcaggatct
    12961 attttgtaat cccggcctgc aagatcaaag caatcagtgg catggcagat ctcatttata
    13021 aacatcaggc agatggctaa catccatttt ttcatttttc cacctctggt gactttatca
    13081 gtaaataatt taacccactc cacaaaaagg ctcaacaggt tggtggttct caccaccaaa
    13141 agcaccacac cccacgcaaa aacaagtttt tgctgatttg ttttttaaat cattagttta
    13201 tgttctaaat aatgtatttt aatttatttt acattattaa atagggtcgt tgacgcccct
    13261 atggcgaccc tatgacgacc cttgttaggg tcagtgctga caattttcat gaaaaacatt
    13321 tggatatatt agggccgccc ctagcagcgc cgtgcgtttt tttatagtat gcccctaggg
    13381 ggcgctgcta ggggtgctct tcttttttca gaccatacta tgagtagatg aaataaaact
    13441 cttatttcag aatctaaaag gtactgtctt atggccagag taaatctgta tatcagtaat
    13501 gaggttcatg aaaaaattaa catgattgtt gaaaagcgtc gtcaggaggg agcaagagat
    13561 aaagatataa gcctttcagg aactgcttca atgcttcttg aattagggct ccgtgtatat
    13621 gacgctcaga tggagcgtaa agagtctgca tttaaccaga cagagtttaa taaacttctt
    13681 cttgaatgtg ttgtaaaaac acagtcaacg gtggcaaaga ttttaggtat tgaatctctc
    13741 agtcctcatg tatccggaaa cccgaagttt gaatatgcca gtatggttga cgatatcaga
    13801 gagaaagtgt ctgttgagat ggaccggttt ttcccaaaaa atgatgatga ataaatgaaa
    13861 atgaagtttg agttggttca aatataggag tttttatgat tcaaaaagat gacaatacgt
    13921 cagataatta gtatattaat tacgtggtta atgccacgtt aaaaattgaa actgaaaatc
    13981 gccgatgcag ggagctgtaa ctccctgcat cgactgtcca tagaatcctt agtggaggtt
    14041 cctatgtgtg cgctggatcg tagagaaagg ccacttaaca gtcaatctgt aaataaatac
    14101 atccttaacg ttcagaatat ctacagaaat tctcccgttc cggtttgtgt ccgtaacaaa
    14161 aaccggaaaa tcctttatgc caatggggct tttattgaac tcttttccag agaagataaa
    14221 cctttatccg gagagagtta tatacgtctg caggttgaaa tttttctttc atcacttgaa
    14281 ctggaatgcc aggctcttgg acatggctct gcattttgtc gtcgttttaa ttttcatggc
    14341 gaaatctatc agataaggat ggagaatgtt tctttttata atgacgaatc tgttgtttta
    14401 tggcaaatta atccgtttcc tgattatcca ttttttgcgt taaatcagag tgtaagtaat
    14461 acaaatactt ctggtcaatt aacgatatgg aatgatcttt ctccaggaac attggttgtt
    14521 ttctcttttt atatgctggg tgttggtcac gcaacaattg ccagagaatt gggtattaca
    14581 gacagagcat ctgaggatcg aattaaacca gttaaacgga aaataaaaga gttttttgaa
    14641 cactttgatt tattcagagt gtcatgtatc tataaaggag aaatagattc gctattaagt
    14701 ataattcgtg aattttatgg tgttaaataa atgttaaata aggcgcgcca attttggcgt
    14761 gttttttttc gccgatttct gtgcaatcat aattttatta gtcgggaaac agaggtgaat
    14821 ttagtgagga ggcgtaacgc gagaggcggt ataagcagaa cggtctcggt gtatctcgat
    14881 gaagatacaa ataaccgact tattaaggcc aaagatcgta gtggaagaag taaaacgata
    14941 gaagtccaga tccgtctcag agatcatctg aaacggtttc ctgatttcta taacgaagaa
    15001 atattcaggg aggtgaccga agagagcgag tcaacattta aagaacttta gtttttatct
    15061 atcaatatta aacagaggaa tcatatggac gccgttttaa gtgttcaggg tgcttctgcg
    15121 cccgtcaaaa agaaatcgtt tttttccaaa ttcactcgtc tgaatatgct tcgcctggct
    15181 cgcgcagtga tcccggctgc tgttctgatg atgttcttcc cgcagctggc gatggccgcc
    15241 ggcagcagtg gtcaggacct gatggcaagc ggtaacacca cggttaaggc gaccttcggt
    15301 aaggactcca gtgttgttaa atgggttgtt ctggctgaag ttctggtcgg tgctgtcatg
    15361 tacatgatga ccaaaaacgt caagttcctg gccggttttg ccatcatctc tgtatttatt
    15421 gctgtgggta tggccgtcgt tggcctctga caggaaataa aacgatgtcg ggagacgaga
    15481 ataaacttaa gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc
    15541 tgccactgga tgaactgatc cccgcagcaa tctgtattgg ctggggtatc acaacatcga
    15601 aatatctgtt cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa
    15661 aagggcgggg cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc
    15721 tgcgtggtat ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact
    15781 gataccagga ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca
    15841 tcgcctttat atttatgtca gtgcttattg ttctcagcct ctctgttaac gtcattcagg
    15901 gggtgaataa ctaccgtctt cagaatgagc aacgcactgc cgtgacgcca atggcattta
    15961 atgccccctt tgccgtgtca cagaacagtg ccgacgcctc ttatttacag cagatggcgc
    16021 tgtcatttat tgccctccgt ctgaatgttt catcagaaac cgtcgatgcc tcacatcagg
    16081 cgcttctgcg atatatccgc ccgggcgcac agaaccagat gaaagttatt ctggctgaag
    16141 aagcgaagcg tattaaaaac gataacgtga actcagcctt tttccagacc agtgttcgtg
    16201 tctggcctca gtatggccgt gtggaaattc gaggtgtgct taaaacctgg attggtgatt
    16261 caaaaccttt cactgatatc aaacattaca tccttattct gaagcgggaa aacggggtga
    16321 cctggctgga taatttcggg gaaacagacg atgagaaaaa ataatacggc aataatattc
    16381 ggcagcctgt ttttttcctg cagcgtgatg gccgcaaacg gtacgctggc ccccaccgtg
    16441 gtgccaatgg tgaacggtgg tcaggccagt attgccatca gcaataccag cccgaatctg
    16501 tttaccgttc ccggtgaccg gattatcgcc gtgaacagtc tggatggtgc cctgaccaat
    16561 aatgagcaga ccgcctccgg cggtgtggtg gttgcatcag ctggattgta tggtgatgct
    16621 catgccggat tagatgcaaa gcccagcctt tttggtgtgc gtgttgatgt tggcgctaag
    16681 gttggtgggc gagccagtat tgacggtagt gatctggact cacatgaagc cagtagtggc
    16741 agcagggttt cccatgatgc tcgccatgat attgacgcta gagccagtaa ggactttaaa
    16801 gaggccagcg attactttac cagtcgcaaa gtcagtgaat ccggcagcca tactgacaat
    16861 aatgccgatt cccgtgtgga ccagctgtct gcagccctga actcagcaaa acagagttac
    16921 gaccagtaca cgacgaatat gacccgcagc catgaatatg ctgaaatggc atcccgtact
    16981 gaaagcatga gtgggcagat gagtgaagac ctatcgcaac agtttgcaca gtatgtgatg
    17041 aaacacgctc cgcaggatgc agaagctatt ctgacaaata ccagttcgcc ggagattgca
    17101 gaacgccgtc gggcaatggc gtggtctttt gtgcaggaac aggtgcagcc tggtgttgat
    17161 aatgcctggc gtgaatcccg tggtgatatt ggtaaaggaa tggagagcgt accttcgggt
    17221 ggtggcagcc aggatattat tgctgaccat cagggacatc aggccattat tgagcaaaga
    17281 acgcaggaca gtaatatccg aaatgatgta aaacatcagg ttgataatat ggtcacagaa
    17341 taccgaggca atatcgggga tactcagagc agcatacatg gcgaagaaaa tattgtagga
    17401 agacagtatt ctgaactaca aaatcaccat aaaacagaag cattatctca gaataataaa
    17461 tatgatgaag agcgagaaag tcaaaagata atacctgagc catcaggaga ggctgtgaaa
    17521 aaaatgattg atggtaaaaa ggaacgactg aaggggccat tatgatcact cactatgata
    17581 taaaaatgga gacacagaaa ctaaaggatg tactctctgt agagggggtt aatatcccgc
    17641 cattattaca ggtcataaag ccaggggggt atgtgttttt atgggtttta ttatggccaa
    17701 catttcttcg tttgctggca gataaagttg atatccgaga tgccggattt gatatatgtt
    17761 ttagtggcgt aatgggtttt atcctttttg ttgccattac taatggaatg atgttgtatc
    17821 ttgctatccc tgaaaaattc agagatgagt cgaaagttat atcttttatg tatgataaga
    17881 acaaaaatta tatcttgtca tttttgattg ctttttcaat ggtttcattt gcccatacac
    17941 tcttatatga gtttttatta attgctttgt ttataatctt tttctttatt tatgctatag
    18001 acataaatcg ttataatctc tcagctatag cctcagtaat agggctgttc aaaaaagagt
    18061 ctgttagtta aaaggatatt ttggatgaaa acaaaaaaat tgatgatggt tgcactggtc
    18121 agttccactc tggccctttc agggtgtggt gcgatgagca cagcaatcaa gaagcgtaac
    18181 cttgaggtga agacccagat gagtgagacc atctggcttg aacccgccag cgaacgcacg
    18241 gtatttctgc agatcaaaaa cacgtctgat aaagacatga gtgggctgca gggcaaaatt
    18301 gctgatgctg tgaaagcaaa agggtatcag gtggtgactt ctccggataa agcctactac
    18361 tggattcagg cgaatgtgct gaaggccgat aagatggatc tgcgggagtc tcagggatgg
    18421 ctgaaccgtg gttatgaagg cgcagctgtt ggtgcagcgt taggtgccgg tattaccgga
    18481 tataattcca attctgccgg tgccacactc ggtgtgggcc ttgctgctgg tctggtgggt
    18541 atggctgcag atgcgatggt ggaagatgtg aactatacca tgatcacgga tgtgcagatt
    18601 gcagagcgta ctaagacaac ggtgacaacg gataatgttg ccgccctgcg tcagggcaca
    18661 tcaggtgcga aaattcagac cagtactgaa acaggtaacc agcataaata tcagacccgt
    18721 gtggtctcca gtgcgaacaa ggttaacctg aaatttgaag aggcgaagcc tgttctcgaa
    18781 gaccagctgg ccaaatcaat cgcaaatatt ctctgattat cttccggagg ctggtctgac
    18841 cggcctcctg actgacgcct cgctttgctc gttgtccaac cccggaaaga aataaaataa
    18901 attttatttc tccccggcgt ttccgaaata aatatgaaaa cactgagcgg ttgtgcaaat
    18961 tctttgtgcg accgtgaagt gttgccggcg aagccggaat ttaacggtta ttaatctttt
    19021 catcattaat aatcagttcg actgactttt ttcttcggaa tatcatcatg agttttaacg
    19081 caaaggatat gacccagggc ggtcagattg cgtccatgcg tatccgcatg ttcagccaga
    19141 tcgccaatat catgctttac tgcctgttta tttttttctg gatactcgtt ggtctggttt
    19201 tatgggtaaa aataagctgg cagacgtttg tgaacggctg tatttactgg tggtgtacca
    19261 cgctggaagg catgcgggat ttaatcaagt cccagccggt atatgagatc cagtattacg
    19321 gcaaaacctt ccggatgaat gctgctcagg tactgcatga taaatatatg atctggtgcg
    19381 gagagcagct atggtccgca ttcgttctgg ccacagttgt ggcactggtt atttgcctga
    19441 ttaccttctt tgttgtctcc tggattctgg ggcgtcaggg taaacaacag agcgaaaatg
    19501 aaatcacagg tggtcgtcag ctgactgaca atccgaaaga ggtggccagg atgctgaaaa
    19561 aagatggcaa ggattccgat atccggattg gcgatctgcc gattatccgg gattctgaaa
    19621 tccagaactt ctgtctgcac ggcactgtcg gggccggtaa gtcggaggtt atccgtcgtc
    19681 tggccaacta cgcccgtcag cgtggtgata tggtggtgat ttatgaccgt tcgggggaat
    19741 ttgttaaaag ttactatgat ccctccatcg ataaaatcct gaatccgctg gatgcacgct
    19801 gtgctgcctg ggatttgtgg aaggagtgcc tgacacagcc agattttgat aataccgcaa
    19861 atactctgat cccgatgggg acaaaagagg acccgttctg gcagggttca ggacgtacca
    19921 tttttgcgga agcggcatac ctgatgcgta atgaccccaa ccgcagctac agcaaactgg
    19981 tggacacact gctttccatc aaaattgaaa agctgcgtac cttcctgcgt aattcaccgg
    20041 cggccaacct ggtggaagag aaaatcgaga aaacggccat atccatccgt gctgtgctga
    20101 ccaactacgt gaaagccatc cgttacctgc aggggattga gcataacggt gagcccttca
    20161 ccatccgtga ctggatgcgt ggtgtccggg aagatcagaa aaacggctgg ctgtttattt
    20221 cgtcgaacgc cgacacccat gcctccctga agccggtgat ctccatgtgg ctgtccattg
    20281 ctattcgtgg tctgctggca atgggagaaa accgtaaccg tcgtgtgtgg tttttctgtg
    20341 acgagttacc cacgttacac aaactgccgg acctggtgga gatcctgccg gaagcccgta
    20401 agttcggtgg ctgttatgtg tttggtatcc agtcctatgc ccagctggaa gatatctacg
    20461 gtgagaaagc ggctgccacg ctgtttgacg tcatgaacac ccgtgccttt ttccgttctc
    20521 ccagccataa gattgcagag ttcgctgccg gtgaaattgg tgagaaagag cacctgaaag
    20581 ccagcgagca gtattcctac ggtgctgatc cggtacgtga cggggtatcg accggtaagg
    20641 atatggagcg tcagacgctg gtcagttatt ccgacattca gtctctgccg gatctgacct
    20701 gttatgtcac cctgcccgga ccgtatccgg cagtaaaact ctccctgaaa tatcagacac
    20761 gaccgaaggt cgctccggag tttattccgc gtgacatcaa cccggaaatg gagaaccgtc
    20821 tgagtgccgt acttgccgca agggaagcag aaggtcgtca gatggccagc ctcttcgaac
    20881 cggatgtccc ggaggttgtt tccggagaag acgtgactca ggctgaacag ccgcaacagc
    20941 cggtgtctcc tgccatcaac gataagaagt cagatgcagg tgtgaatgtt ccggcagggg
    21001 ggatcgagca ggagctgaaa atgaaaccgg aagaagagat ggaacagcaa ctgccacccg
    21061 ggatcagtga atccggtgaa gtggtggata tggccgttta tgaggcatgg caacgggagc
    21121 agaatccgga tatccagcag aagatgcagc gtcgtgaaga ggtgaacatc aatgtgcacc
    21181 gggagcgcgg ggaggatgtt gagccgggag atgatttctg atgaacaggt cagctccagt
    21241 cttcaattct cagaccgtcc acacgttcaa attcccgggt gttattagtc acaataatca
    21301 gtccccgact gcgggcatga cctgcgatca tttgatcaaa tggcccgaca gggcgtccct
    21361 gacgggcaag ttctgctctt atctggccgc tgtgtgtggc tgcggcagcg tcgtaatcca
    21421 gaacatcaat gcgggaaaca aatccctcga tcacagcgag attacgttca ggcatctggc
    21481 ttttttctgc accatatatc agctccatca gggtgaccga actgatacac attctcccct
    21541 ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt ctttatcgtg aaaatgcaga
    21601 tgttggtatc gagcataaac ttcagcatca gaatgactcc ctttcctgca tgccgggctg
    21661 ttccctgtta tccataaaat cggcgctgac gttgtgtccg tcgaaccatt cgtcccatgt
    21721 ctctccggct ggcgtaatga ttctggttcg tccgacagca atcacttcaa cgcgttttac
    21781 gttttccggc aatgcaaccg cttttggcag tctgaccgcc tggctgcggt tgctgaaaaa
    21841 tacggtggtt tccattattt gcctccttat gtggatatgt cttgtgtata tccattgtgg
    21901 gattgatacc gggatatgtc aaagggatat acgtttatga tgagtatcgc gcaggtcaga
    21961 tcggccggaa gtgccggtaa ctattatacc gacaaggata attactatgt gctgggcagc
    22021 atgggagaac gctgggccgg caggggggct gaacagctgg ggctgcaggg cagcgtcgat
    22081 aaggatgttt ttacccgtct tctggagggg aagctgccgg acggagcgga tttaagccgc
    22141 atgcaggatg gcagtaataa gcatcgtccc ggctacgacc tgaccttctc cgcccccaaa
    22201 agtgtctccg tgatggccat gttaggcggt gataaacgtc ttattgatgc acataaccag
    22261 gccgtggatt ttgctgtccg tcaggtggag gcgctggctt ccacacgggt tatgacggac
    22321 ggacagtcag aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc
    22381 agtcgcgatc aggaaccaca gttacacaca catgcggtag tggccaatgt cacgcagcat
    22441 aacggcgagt ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat
    22501 gtgtacgcta atcagattgc cttcggcagg ctctaccggg aaaaactgaa agagcaggtt
    22561 gaggcgctgg gctatgaaac ggaagtggtt ggtaagcacg gtatgtggga gatgccgggc
    22621 gtaccggtgg aggccttctc cggacgctca caggctatcc gggaggccgt cggggaagac
    22681 gcctcgctga aatcccggga tgtggcggca ctggatacgc gaaaatccaa acagcacgtc
    22741 gatcctgaag tcagaatggc tgaatggatg cagacgctga aggaaaccgg gttcgacatc
    22801 cgggcgtatc gtgacgcggc ggaccaacgg gcggagaccc gcacgcagac gcccggaccc
    22861 gcttcacagg acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt
    22921 gaacgcaaag tgcagtttac gtacacggac gtgctggcca gaacggtcgg catactgccg
    22981 ccggaaaatg gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag
    23041 cagcttatcc ccctcgatcg tgagaagggg ctgttcacgt ccgggattca tgtgctcgat
    23101 gagctgtcag tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat
    23161 ccggagaaaa gtgtcccccg gacggccggt tacagcgatg cggtcagtgt tctggcacag
    23221 gatcgcccgt cgctggctat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg
    23281 gtggcggagc tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgccgct
    23341 gaccgtcgct cgcagatgaa cctgaagcag gatgaacggc tgtccggtga gctgataacc
    23401 ggacgtcgtc agctgcagga gggcatggcc ttcacgccgg gcaatactgt tatcgttgac
    23461 cagggcgaaa aactctccct gaaagagacg ttaaccctgc tggacggtgc cgcacgtcat
    23521 aacgtacagg tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg
    23581 gccatgaaag atgctggggt gaacacatat cgctggcagg ggggagaaca gcggccggcc
    23641 accatcatca gtgaaccgga ccgtaatgtc cgctatgccc ggctggcagg agattttgct
    23701 gccagcgtga aagccggaga agagagcgtg gcacaggtca gcggggtacg ggaacaggcc
    23761 atactgacac aggccattcg cagtgagctg aaaacacagg gcgtgctcgg acgcccggag
    23821 gtaaccatga ccgccctgtc gccggtctgg ctggacagcc ggagccgtta tctgcgggat
    23881 atgtaccgtc cggggatggt gatggagcag tggaacccgg agacacgcag tcatgaccgc
    23941 tatgttatcg accgggtgac ggcgcagagt aacagcctga ccctgcggga tgcgcagggt
    24001 gaaacgcagg tggtgcgtat ttcctccctg gacagcagct ggtcgctgtt ccggccggaa
    24061 aaaatgccgg tggcagacgg cgagcgactg agggtgacag ggaaaattcc cggactccgc
    24121 gtctccggcg gtgaccgcct gcaggtggca tccgtcagtg aagatgcgat gacggttgtt
    24181 gtgccggggc gggctgaacc ggcctccctg cctgtgagcg attcaccatt catggcactg
    24241 aagctagaga acggctgggt ggaaacgccc gggcattccg tcagcgacag tgcgaaggtt
    24301 tttgcctccg tcacacagat ggcaatggac aacgccaccc tgaacggtct ggcccgcagc
    24361 ggtcgtgatg tccggctgta ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc
    24421 cgccatccct cctttacggt ggtttctgag cagataaagg cgcgtgccgg tgagacattg
    24481 ctggaaaccg ctatcagtct gcagaaaacc gggcttcaca cgccggcaca gcaggctatt
    24541 catctggcgc ttcctgtggt ggaaagtaaa aacctggcct tcagcatggt ggacctgctg
    24601 acagaggcga agtcgtttgc tgcagaagga accagtttta ctgaactggg aggggaaatc
    24661 aatgcgcaga taaaacgcgg tgatttactg tatgtggatg tggcaaaagg ctatggcaca
    24721 ggcctgctgg tttcccgtgc gtcgtatgag gcagaaaaga gcattcttcg ccatattctc
    24781 gaaggtaagg aggcggtcac gccactgatg gagagagtac ctggcgaact catggagaaa
    24841 ctgacatcag gacagcgtgc cgctacccgc atgatactgg aaacgtccga ccgtttcacg
    24901 gtggtgcagg gctatgccgg tgtgggtaag accacacagt tccgggcggt gatgtcagcc
    24961 gtgaacatgc tgccggagag tgaacgtccc cgtgtcgtgg ggctggggcc cacgcaccgt
    25021 gcggtcggtg agatgcgcag cgccggcgtg gatgcacaga cgctggcgtc ctttctgcat
    25081 gacacgcagc tgctgcagcg cagcggagaa acgccgaatt tcagcaacac gctgttcctg
    25141 ctcgatgaga gctctatggt gggcaatacc gacatggcac gggcatacgc cctgattgcg
    25201 gccggtggcg gtcgtgccgt ggccagtggt gacacggacc agttgcaggc catcgcgccc
    25261 ggtcagcctt tccgtctcca gcagacgcgc agtgctgccg atgtggccat catgaaggag
    25321 attgtgcgcc agacgccgga gctgcgggag gcggtataca gcctgattaa ccgggatgtg
    25381 gaaagggcac tgtccgggct tgagagtgtg aaaccgtctc aggtgccacg tcaggagggc
    25441 gcatgggtac cggagcactc cgtgacgcag ttcagtcaca gccaggaagc gaaactggca
    25501 gaagcgcagc agaaggcgat gctgaaaggt gaggcttttc cggatattcc catgacactg
    25561 tatgaagcca ttgtccgcga ttataccggc aggacgccgg aagcacggga gcagacgctg
    25621 attgtcacgc atctgaatga ggaccggcgc gtactgaaca gcatgattca tgatgcacgg
    25681 gaaaaggccg gtgagctggg aaaagagcag gtcatggtgc ctgtcctgaa cacagcgaat
    25741 atacgcgacg gggagttgcg tcgtctctcc acctgggaga cacatcggga cgcacttgcc
    25801 ctggtggata atgtgtatca ccggattgcc ggtatcagca aggatgacgg gctgataacc
    25861 ctgcaggatg cggaaggtaa cacgcggctg atttcgcccc gggaggcggt ggctgaaggc
    25921 gtcacactgt acactccgga caccatccgg gtgggaaccg gtgaccggat gcgcttcacg
    25981 aagagtgacc gggagcgcgg ttatgtggcc aacagcgtct ggacggtgac agcggtttcc
    26041 ggtgacagtg tcacgctgtc ggacggacag cagacccggg tgattcgccc cggtcaggag
    26101 cgggcagagc aacatattga cctggcctat gccatcaccg cccacggtgc gcagggggca
    26161 agtgaaacct ttgccattgc gcttgaaggc acggaaggta accggaaaca gatggccggc
    26221 tttgagtcag cctacgtggc cctgtcgcgt atgaagcagc atgtgcaggt gtataccgat
    26281 aaccgtcagg gctggacgga tgccattaac aatgccgtac agaaaggaac tgcccacgat
    26341 gtgctagagc cgaaatcgga ccgggaggtc atgaatgcag agcggctgtt cagtacggcg
    26401 cgggagctgc gggacgtggt ggccggccgt gccgttctcc gtcaggcagg gctggccggg
    26461 ggagacagtc ctgcacggtt tattgcaccc gggcgcaaat atccgcagcc gtatgtggca
    26521 ctgccggctt ttgaccgtaa cggcaagtca gctggtatct ggctgaaccc gctgaccacg
    26581 gatgacggaa acgggctgcg gggcttcagt ggtgaaggcc gggtgaaagg cagcggggat
    26641 gcgcagttcg tggccctgca gggcagccgt aacggagaga gcctgctggc tgataatatg
    26701 caggatggtg tccggattgc ccgtgataat cctgacagcg gtgtggtggt aagaatcgcc
    26761 ggtgaaggtc gtccgtggaa tcccggtacc atcaccggtg gtcgcgtgtg gggggatatc
    26821 ccggacaaca gcgttcagcc gggagccgga aatggcgaac cggtcacggc agaggtactg
    26881 gcacagcggc aggctgaaga ggccatccgc cgtgaaacgg aacgccgtgc ggatgaaatt
    26941 gtccgtaaaa tggcagagaa caaacctgac ctgccggacg gcaaaacaga gcaggctgtc
    27001 agggatattg ccgggctgga gcgtgaccgg tctgccatat ctgaacggga agccgcgctg
    27061 ccggagagtg tactgcgtga accacaacgg gtgcgggaag cggtccggga ggttgcccgg
    27121 gaaaatctgc tgcaggagcg actgcagcag atggagcggg atatggttcg tgacctgcag
    27181 aaagagaaaa ccctgggcgg agactgacac aggaagataa acgctgatga caacggataa
    27241 cacgaacaca acacgtaatg attcactggc tgcccggacc gatacctggt tgcagtcgtt
    27301 gctggtctgg tcacccggac agcgggatat catcaaaacg gtggcactgg tgctgatggt
    27361 tctggaccat gcaaaccgca ttctgcatct ggaccagtcg tggatgtttc tggtcgggcg
    27421 tggggccttt ccgctgtttg ccctggtgtg ggggctgaat ctgtcccgtc atacgcatat
    27481 ccggcaggag gccattaacc ggctgtgggg atgggcggtg attgcccagt tcgcatatta
    27541 tctggccggc tttccctggt atgaggggaa tatcctgttt gcctttgcag ttgcggcaca
    27601 ggtgctgaca tggtgtgaaa cgcgcacctg gtggcgttcc gcagagacca tgttgcttct
    27661 ggcaatgtgg ttgcctttct ccggcaccag ttacggcatt gccgggctgc tgatgctggc
    27721 agtcagccac aggctgtacc gggcggaaga cagaatggaa cgtctggcgc tggtcgcctg
    27781 cctgctggct gttattccgg cgcttaacct tgccaccagt gatgcagcgg cggtggccgg
    27841 ccttgtgatg acggtgctga ctgttggtct ggtgtcctgt acagggaaat cactaccccg
    27901 tttctggtac ggggattttt tcccgacgtt ctatgcctgt catcttactg tgctgggcgt
    27961 tctggctgtg tgactggtat gatgctggcg ctgattctgc ttattaataa acaacataac
    28021 acaggatatt tatgaaaatt acggatcaca aactccctga aggtattgct ctgacatttc
    28081 gtgtcccgga aggaaatata aaacatcctt tgattctctt atgccatgga ttttgcggta
    28141 tccggaatgt tctgctgcct tcctttgcga atgcgtttac agaagccggg tttgcaacga
    28201 ttacttttga ttatcgtgga ttcggtgaga gtgaagggga acgaggtcgt ctggttccgg
    28261 caatgcagac cgaagatatt atttcagtta tcaattgggc agaaaagcag gtatgtattg
    28321 ataatcagcg tataggatta tggggaacat cgctgggtgg gtgtcatgta ttcaatgcag
    28381 ctgcgcagga taaaagagtg aaaagtattg tcagccagct ggcttttgct gacggtgagg
    28441 ttctggttac cggagaaatg aatgaactgg agaaagcttc ttttctgtcc acgctgaata
    28501 aaatggcaga aaagaagaaa aataccggga aagagatgtt tgtgggggtt acccgggtac
    28561 tgagtgataa tgaatcaaag gtattcttcg agaaggtaaa aggtcagtat cctgaaatgg
    28621 atataaaaat acctttcctt acggttatgg agacgttgca atataaaccg gcagagagcg
    28681 cagccaaagt acagtgccca gttttggttg ttattgccgg ccaggatagt gttaatccac
    28741 cagaacaggg aagagcctta tatgatgctg ttgcttctgg tactaaggaa ttatacgagg
    28801 aagctgacgc atgccattat gatatttatg aaggcgcgtt ttttgaacgt gtggcggcag
    28861 tacagacgca atggtttaaa aaacatcttt aattgtcggt attaatatgt cagaggtcgt
    28921 tgaggtcagc ttaatatcat agtgagtagt ttcaacgacc tgggggacat attttcacaa
    28981 aggtcagggc aatcatgaca gagcagaaac gaccggtact gacactgaaa cggaaaacgg
    29041 aaggggaaac accgacccgg agccggaaaa ccatcatcaa tgtcaccacg ccaccaaaat
    29101 ggaaggtgaa aaagcagaaa ctggcggaga aggctgcccg ggaagcagag ctggcggcga
    29161 agaaagcaca ggccagacag gcgctgtcca tttatctgac gctgccctcg ctggatgagg
    29221 cggtgaacac tctgaaaccc tggtggccgg gattgtttga cggtaacacg ccccggcttc
    29281 ttgcctgtgg tatccgggag gttttactgg atgaggtgtt tcagcggaat atcccgctct
    29341 ctcataaaaa actcagccgg gcgctgaaag ccatcacccg ttcggaaagt tatctgtgtg
    29401 caatgaaagc cggcgcctgc cgctatgata cggaaggcta tgtgacagaa catatcacac
    29461 aggaagaaga acagtatgcg caggcgcgcc tggaaaaggt gcgcagacag aaccggatca
    29521 aggatgagct gcgggcgatc ctggcagaat aataaaaacg ccctctgtat gacagagggc
    29581 aacagcgaaa aaatcgggaa aacagacatc cagagttttg atggagaaaa acacccaacc
    29641 gttcagggat gtttcataat gtttctgaca taatccggga gccgggtcaa gaggggggat
    29701 aaaaaatggc tgtatccagt ggattatctg agcacagcgg tatttatcgc tatcagcgcg
    29761 tggggcgtca tgccgtccgt caggagattc gtggtttttt cagggaagcc gctgaggcct
    29821 ggcagaatgc ggcccggctg gcaccctgtg aagcatggcg ggattttgcc ctgaagagag
    29881 catctgcatg ccggtgtaag tgccgataaa aataaccccc gttgcggagc gccgggggat
    29941 cagacttata tggcagatcg ttattattat agtggtgtaa gaaggagttg ccctgtcttt
    30001 caaacggaca tcacagaatt aacatatttc acatcccggg taagccaaca gggataaaaa
    30061 atgtgatgaa tttcaagaag cagaaggtga cagtggcatg cataacagta cggttatgtc
    30121 cggtgaatct gacagacagc cgggctgtat tcgcggcagg ataaagaaaa accgggagat
    30181 gactctcccg gtccgtctta tcgctaatcc cctgagcagg gcatgtatga cttatctatg
    30241 accgttatat tcagcgccac atccggtagc ctgcgatata acacagttat cctatcagta
    30301 agagcaggta tgatgaatac acggagatta aaaatataat ctccgtcaca tgttaccccc
    30361 ggaaaccggt aacgccgtca cgggaaatac gtgacagaaa aagcagcctc cgttactcca
    30421 ggtatcagaa ggctgaataa tgcatgagac tttctttctt atgtgtctgg tcaggcgggg
    30481 tgaccagcgt ttttagtaac acatagccat atgtgtatta agccacctca tcttattttg
    30541 tcgaattgat cacaattcaa cggtttattt ttggcgtgat gcgtgagccg ttattcatcc
    30601 cggcatggaa ttcatgcaat cacgaaaagt aatcagtgac ggagagtaaa atgaagaaat
    30661 atataccagt ggtattattt gttttttcat gtcaggtatt ttctgctgat attcatggac
    30721 gtgttgtgcg tgttctggat ggcgatacca ttgacgtcat gttatctcag catccggtgc
    30781 gtgtccgtct tgttaatatt gatgcacctg aaaagaaaca ggactatggc cggtggtctg
    30841 aaaaaataat gaaatctctg gttgcgggaa aaacggttac tgtcacatat tttcagcgag
    30901 atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggtatgaat gttaatcagt
    30961 ttatggtccg tgcaggcgct gcatgggttt atgagcagta caacaccgat cctgttcttc
    31021 ccgtcctgca ggaagaagca cgacagcaga aacgtggtct gtgggcagac agcgctcctg
    31081 ttccgccctg ggtatggcgc tcacggaaat aatgatattt ctgtggctat tgagttctca
    31141 ttgaaatgcg atatttaaaa gcatgaagca tattacatta ttaacttatt tctctgttaa
    31201 tgaaaggtga acaaataaac tatcaggcag tatcagaatt aatcacatcg tctaatcata
    31261 atgttcttat tgtggggggc tcagcttctg aagttgatag ctttttaaat aaactgaata
    31321 ttactgatta taagtattat gatttcagcc agatttacag ttgttctgac agaacattaa
    31381 atgattatgc ggttattttt atcagggatg cactgaacgc cagtgagcat attattattt
    31441 ttaattgtac aggatggcct gatttgaata atgaatctgc cgtcatgcag tttgccagag
    31501 tggcacgaaa atcaggaaaa caactgatag tcgcagtaag agaacaggat atgaaaaaaa
    31561 tggaagcaga gtttggaagg atcataaaaa tccactgacg ccttcgcgtt gctcagttgt
    31621 ccaaccccgg ggacgggaaa aagcaagttt tccccgcccc cggcgtttct gtagtgaaaa
    31681 gcgggaaatg gagggaatgg tcagtaacag ggaatgtggt attatcgcgg cgggtgtctg
    31741 agcctttctg gttcaggcaa gacgcaggta ccagaaatgc gaagacccca cttgttaatc
    31801 cattaactcg tgaggcctgc atgaagtact tgaacactac tgattgtagc ctcttccttg
    31861 cagagaggtc aaagtttatg acgaaatatg cccttatcgg gttgctcgcc gtgtgcgcca
    31921 cggtgttgtg tttttcactg atattcaggg aacggttatg tgagctgaat attcacaggg
    31981 gaaatacagt ggtgcaggta actctggcct acgaagcacg gaagtaagct gccgggtggg
    32041 gacggaagtc cccgctttcc ggaagtgtga ggtatttcag gggcagacac ccgacatgcc
    32101 agaaaccgcc ggtcccgccc ggggccggca cacaggttaa ggcatttcct gtttttcagc
    32161 catttcgcaa caaaaatcac attaaacaac agtaatcacc gttgatttgt gcgccaacac
    32221 agatcttcgt cacaattctc aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa
    32281 cgatccctgt ttgagaattg aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc
    32341 tcattgagtc aaaagcggtt tgtgcgcaga ggtaagccta tgactgactc tgagaaacaa
    32401 atggccgctg ttgcaagaaa acgtcttaca cacaaagaga taaaagtttt tgtcaaaaat
    32461 cctctgaaag atctcatggt tgagtactgc gagagagagg ggataacaca ggctcagttc
    32521 gttgagaaaa tcatcaaaga tgaactgcag agactggaca tactaaagta aagactttac
    32581 ttttggcgta gcatgctaga ttactgatcg tttaaggaat tttatggcgg gccacgccgt
    32641 aaggtggcag ggaactggtt ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg
    32701 ataatcttct ttaactttgg cgagtcagaa agattaccgg ggccatctaa aaaccgtata
    32761 gccaacaatt cagctatgcg gggagtatag ttatatgccc ggaaaagttc aagacttctt
    32821 tctgtgctcg ctccttctgc gcattgtaag tgcaggatgg tgtgactgat cttcaacaaa
    32881 cgtattaccg ccaggtaaag aacccgaatc cggtgtttac accccgtaaa ggtgccggaa
    32941 cgctgaagtt ctgcgaaaaa ctgatggaaa aggcggtggg cttcacctcc cgttttgatt
    33001 tcgccattca tgtggcgcat gcccgttcgc gtggtctgcg tcgacgcatg ccaccggtgc
    33061 tgcgtcgacg ggctattgat gcgctgctgc aggggctgtg tttccattat gacccgctgg
    33121 ccaaccgcgc ccagtgttcc attaccacac tggccattga gtgcggactg gcgacagagt
    33181 ccggtgcagg aaaactctcc atcacccggg ccacccgggc cctgacgttc ctgtcagagc
    33241 tgggactgat tacctaccag acggaatatg acccgcttat cgggtgctac attccgaccg
    33301 atatcacgtt cacaccggcg ctgtttgccg cccttgatgt gtctgaggat gcagtggcag
    33361 ctgcgcgccg cagccgtgtg gtatgggaaa acagacagcg caaaaagcag gggctggata
    33421 ccctgggtat ggatgagctg atagcgaaag cctggcgttt tgtccgtgag cgtttccgca
    33481 gttaccagac agagcttaag tcccacggca tgaaacgtgc ccgtgcgcgc cgcgatgcag
    33541 gcagaacccg ccaggacatt gtcaccctgg tgaaacggca gctgacgcgt gaaatcgcgg
    33601 aagggcgttt ccggggaagc ctggaggccg tgaagcgcga aatcgaccgt cgtgtgaagg
    33661 aacgcatgat aatgtcgcgt aacaacaact acacacggct ggccactgcc tccccctgag
    33721 ttttacctca tctgaataat ccggcctgcg ccggaggcat ctgcacgcct gaagcctgtc
    33781 agcgaacaaa aaaacagcac cacatacaaa aaacaatctc atcatcccgc ttctggtgca
    33841 tccggccccc ctgttttcga tacaaaacat gcctcactga cggggaattt tgcttatcca
    33901 catttaactg caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgccagc
    33961 cgtcagcgtt acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta
    34021 cttaattaca ttcatttaaa aagaaaacct attcattgcc tgtcctgtgg acagacagat
    34081 atgcacctcc caccgcaagc ggcgggcccc aaccggagcc attttagtta caacactcaa
    34141 atcaaccacc gggaaaactc cggtccagcg cagaaccgaa accacaaagc ccctccctca
    34201 taactaaaaa gcggccccgc cccggcccaa agggccggaa caga
//
LOCUS       DAZFVO020000036        34236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFVO020000036 DAZFVO020000000
VERSION     DAZFVO020000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.036"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..704
                     /locus_tag="ACHKNB_RS08145"
                     /old_locus_tag="pgaptmp_001629"
     CDS             <1..704
                     /locus_tag="ACHKNB_RS08145"
                     /old_locus_tag="pgaptmp_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HGU4195162.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKQIARLAEQSTD"
     gene            803..1816
                     /gene="ybjS"
                     /locus_tag="ACHKNB_RS08150"
                     /old_locus_tag="pgaptmp_001630"
     CDS             803..1816
                     /gene="ybjS"
                     /locus_tag="ACHKNB_RS08150"
                     /old_locus_tag="pgaptmp_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4195163.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1813..2643)
                     /gene="amiD"
                     /locus_tag="ACHKNB_RS08155"
                     /old_locus_tag="pgaptmp_001631"
     CDS             complement(1813..2643)
                     /gene="amiD"
                     /locus_tag="ACHKNB_RS08155"
                     /old_locus_tag="pgaptmp_001631"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HGU4195164.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2640..2963)
                     /gene="ybjQ"
                     /locus_tag="ACHKNB_RS08160"
                     /old_locus_tag="pgaptmp_001632"
     CDS             complement(2640..2963)
                     /gene="ybjQ"
                     /locus_tag="ACHKNB_RS08160"
                     /old_locus_tag="pgaptmp_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HGU4195165.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3089..3604
                     /gene="ybjP"
                     /locus_tag="ACHKNB_RS08165"
                     /old_locus_tag="pgaptmp_001633"
     CDS             3089..3604
                     /gene="ybjP"
                     /locus_tag="ACHKNB_RS08165"
                     /old_locus_tag="pgaptmp_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4195166.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3822..4550
                     /gene="artP"
                     /locus_tag="ACHKNB_RS08170"
                     /old_locus_tag="pgaptmp_001634"
     CDS             3822..4550
                     /gene="artP"
                     /locus_tag="ACHKNB_RS08170"
                     /old_locus_tag="pgaptmp_001634"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HGU4195167.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4568..5299
                     /gene="artI"
                     /locus_tag="ACHKNB_RS08175"
                     /old_locus_tag="pgaptmp_001635"
     CDS             4568..5299
                     /gene="artI"
                     /locus_tag="ACHKNB_RS08175"
                     /old_locus_tag="pgaptmp_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HGU4195168.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5306..6022
                     /gene="artQ"
                     /locus_tag="ACHKNB_RS08180"
                     /old_locus_tag="pgaptmp_001636"
     CDS             5306..6022
                     /gene="artQ"
                     /locus_tag="ACHKNB_RS08180"
                     /old_locus_tag="pgaptmp_001636"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HGU4195169.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6022..6690
                     /gene="artM"
                     /locus_tag="ACHKNB_RS08185"
                     /old_locus_tag="pgaptmp_001637"
     CDS             6022..6690
                     /gene="artM"
                     /locus_tag="ACHKNB_RS08185"
                     /old_locus_tag="pgaptmp_001637"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HGU4195170.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6982..7713
                     /gene="artJ"
                     /locus_tag="ACHKNB_RS08190"
                     /old_locus_tag="pgaptmp_001638"
     CDS             6982..7713
                     /gene="artJ"
                     /locus_tag="ACHKNB_RS08190"
                     /old_locus_tag="pgaptmp_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HGU4195171.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7888..9015)
                     /gene="rlmC"
                     /locus_tag="ACHKNB_RS08195"
                     /old_locus_tag="pgaptmp_001639"
     CDS             complement(7888..9015)
                     /gene="rlmC"
                     /locus_tag="ACHKNB_RS08195"
                     /old_locus_tag="pgaptmp_001639"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HGU4195172.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVEHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(9056..9544)
                     /gene="ybjO"
                     /locus_tag="ACHKNB_RS08200"
                     /old_locus_tag="pgaptmp_001640"
     CDS             complement(9056..9544)
                     /gene="ybjO"
                     /locus_tag="ACHKNB_RS08200"
                     /old_locus_tag="pgaptmp_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HGU4195173.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9604..10449)
                     /gene="potI"
                     /locus_tag="ACHKNB_RS08205"
                     /old_locus_tag="pgaptmp_001641"
     CDS             complement(9604..10449)
                     /gene="potI"
                     /locus_tag="ACHKNB_RS08205"
                     /old_locus_tag="pgaptmp_001641"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HGU4195174.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(10446..11399)
                     /gene="potH"
                     /locus_tag="ACHKNB_RS08210"
                     /old_locus_tag="pgaptmp_001642"
     CDS             complement(10446..11399)
                     /gene="potH"
                     /locus_tag="ACHKNB_RS08210"
                     /old_locus_tag="pgaptmp_001642"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HGU4195175.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(11409..12542)
                     /gene="potG"
                     /locus_tag="ACHKNB_RS08215"
                     /old_locus_tag="pgaptmp_001643"
     CDS             complement(11409..12542)
                     /gene="potG"
                     /locus_tag="ACHKNB_RS08215"
                     /old_locus_tag="pgaptmp_001643"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HGU4195176.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12637..13749)
                     /gene="potF"
                     /locus_tag="ACHKNB_RS08220"
                     /old_locus_tag="pgaptmp_001644"
     CDS             complement(12637..13749)
                     /gene="potF"
                     /locus_tag="ACHKNB_RS08220"
                     /old_locus_tag="pgaptmp_001644"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HGU4195177.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     NLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14100..14576)
                     /gene="ybjN"
                     /locus_tag="ACHKNB_RS08225"
                     /old_locus_tag="pgaptmp_001645"
     CDS             complement(14100..14576)
                     /gene="ybjN"
                     /locus_tag="ACHKNB_RS08225"
                     /old_locus_tag="pgaptmp_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HGU4195178.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14664..15566)
                     /gene="rimK"
                     /locus_tag="ACHKNB_RS08230"
                     /old_locus_tag="pgaptmp_001646"
     CDS             complement(14664..15566)
                     /gene="rimK"
                     /locus_tag="ACHKNB_RS08230"
                     /old_locus_tag="pgaptmp_001646"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HGU4195179.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15627..16349)
                     /gene="nfsA"
                     /locus_tag="ACHKNB_RS08235"
                     /old_locus_tag="pgaptmp_001647"
     CDS             complement(15627..16349)
                     /gene="nfsA"
                     /locus_tag="ACHKNB_RS08235"
                     /old_locus_tag="pgaptmp_001647"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HGU4195180.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISDAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16333..16620)
                     /gene="ybjC"
                     /locus_tag="ACHKNB_RS08240"
                     /old_locus_tag="pgaptmp_001648"
     CDS             complement(16333..16620)
                     /gene="ybjC"
                     /locus_tag="ACHKNB_RS08240"
                     /old_locus_tag="pgaptmp_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HGU4195181.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            16780..17037
                     /gene="grxA"
                     /locus_tag="ACHKNB_RS08245"
                     /old_locus_tag="pgaptmp_001649"
     CDS             16780..17037
                     /gene="grxA"
                     /locus_tag="ACHKNB_RS08245"
                     /old_locus_tag="pgaptmp_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HGU4195182.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(17067..17444)
                     /gene="ybjM"
                     /locus_tag="ACHKNB_RS08250"
                     /old_locus_tag="pgaptmp_001650"
     CDS             complement(17067..17444)
                     /gene="ybjM"
                     /locus_tag="ACHKNB_RS08250"
                     /old_locus_tag="pgaptmp_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HGU4195183.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17714..19399
                     /gene="ybjL"
                     /locus_tag="ACHKNB_RS08255"
                     /old_locus_tag="pgaptmp_001651"
     CDS             17714..19399
                     /gene="ybjL"
                     /locus_tag="ACHKNB_RS08255"
                     /old_locus_tag="pgaptmp_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HGU4195184.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19571..20110)
                     /gene="rcdA"
                     /locus_tag="ACHKNB_RS08260"
                     /old_locus_tag="pgaptmp_001652"
     CDS             complement(19571..20110)
                     /gene="rcdA"
                     /locus_tag="ACHKNB_RS08260"
                     /old_locus_tag="pgaptmp_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HGU4195185.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAGL"
     gene            20194..21402
                     /gene="ybjJ"
                     /locus_tag="ACHKNB_RS08265"
                     /old_locus_tag="pgaptmp_001653"
     CDS             20194..21402
                     /gene="ybjJ"
                     /locus_tag="ACHKNB_RS08265"
                     /old_locus_tag="pgaptmp_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195186.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFIDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21402..22217
                     /gene="ybjI"
                     /locus_tag="ACHKNB_RS08270"
                     /old_locus_tag="pgaptmp_001654"
     CDS             21402..22217
                     /gene="ybjI"
                     /locus_tag="ACHKNB_RS08270"
                     /old_locus_tag="pgaptmp_001654"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HGU4195187.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            22303..22587
                     /gene="ybjH"
                     /locus_tag="ACHKNB_RS08275"
                     /old_locus_tag="pgaptmp_001655"
     CDS             22303..22587
                     /gene="ybjH"
                     /locus_tag="ACHKNB_RS08275"
                     /old_locus_tag="pgaptmp_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HGU4195188.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22628..23860)
                     /gene="mdfA"
                     /locus_tag="ACHKNB_RS08280"
                     /old_locus_tag="pgaptmp_001656"
     CDS             complement(22628..23860)
                     /gene="mdfA"
                     /locus_tag="ACHKNB_RS08280"
                     /old_locus_tag="pgaptmp_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HGU4195189.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            24145..24741
                     /gene="ybjG"
                     /locus_tag="ACHKNB_RS08285"
                     /old_locus_tag="pgaptmp_001657"
     CDS             24145..24741
                     /gene="ybjG"
                     /locus_tag="ACHKNB_RS08285"
                     /old_locus_tag="pgaptmp_001657"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4195190.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            24799..25557
                     /gene="deoR"
                     /locus_tag="ACHKNB_RS08290"
                     /old_locus_tag="pgaptmp_001658"
     CDS             24799..25557
                     /gene="deoR"
                     /locus_tag="ACHKNB_RS08290"
                     /old_locus_tag="pgaptmp_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HGU4195191.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25604..26806)
                     /gene="dacC"
                     /locus_tag="ACHKNB_RS08295"
                     /old_locus_tag="pgaptmp_001659"
     CDS             complement(25604..26806)
                     /gene="dacC"
                     /locus_tag="ACHKNB_RS08295"
                     /old_locus_tag="pgaptmp_001659"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HGU4195192.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            27053..27679
                     /gene="gstB"
                     /locus_tag="ACHKNB_RS08300"
                     /old_locus_tag="pgaptmp_001660"
     CDS             27053..27679
                     /gene="gstB"
                     /locus_tag="ACHKNB_RS08300"
                     /old_locus_tag="pgaptmp_001660"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HGU4195193.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27676..28791)
                     /gene="yliI"
                     /locus_tag="ACHKNB_RS08305"
                     /old_locus_tag="pgaptmp_001661"
     CDS             complement(27676..28791)
                     /gene="yliI"
                     /locus_tag="ACHKNB_RS08305"
                     /old_locus_tag="pgaptmp_001661"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HGU4195194.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28902..29285)
                     /gene="bssR"
                     /locus_tag="ACHKNB_RS08310"
                     /old_locus_tag="pgaptmp_001662"
     CDS             complement(28902..29285)
                     /gene="bssR"
                     /locus_tag="ACHKNB_RS08310"
                     /old_locus_tag="pgaptmp_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HGU4195195.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29498..30823
                     /gene="rimO"
                     /locus_tag="ACHKNB_RS08315"
                     /old_locus_tag="pgaptmp_001663"
     CDS             29498..30823
                     /gene="rimO"
                     /locus_tag="ACHKNB_RS08315"
                     /old_locus_tag="pgaptmp_001663"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HGU4195196.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(30870..32198)
                     /gene="dgcI"
                     /locus_tag="ACHKNB_RS08320"
                     /old_locus_tag="pgaptmp_001664"
     CDS             complement(30870..32198)
                     /gene="dgcI"
                     /locus_tag="ACHKNB_RS08320"
                     /old_locus_tag="pgaptmp_001664"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4195197.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDKFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(32206..>34236)
                     /locus_tag="ACHKNB_RS08325"
                     /old_locus_tag="pgaptmp_001665"
     CDS             complement(32206..>34236)
                     /locus_tag="ACHKNB_RS08325"
                     /old_locus_tag="pgaptmp_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4195198.1"
                     /translation="YYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWS
                     VASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLD
                     QISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNA
                     LFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLA
                     FLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKN
                     MAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTF
                     NADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKL
                     KLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTR
                     AILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASL
                     KDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNI
                     EQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLA
                     RYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         8571 a         8873 c         8359 g         8433 t
ORIGIN      
        1 cggtgtggtt tctcaatgtg attacttccg taccgcccac caccgccagg gcgttgattc
       61 aggggctgaa acacgatctg ctggcggatg ataccgcgct acgtgcactc atcccacaac
      121 ggctgatcgc tttcgatgac gcggtacgta gcacgttgaa agaggaggaa aaactggtca
      181 actccagcga ctggggctac gacgctcagg cctttgcccg ctggcgaccg gagtacggtt
      241 attttgccaa acaggcgggg tttaccgtta aaacgtccgc cagccttgct gctttatggc
      301 aggtagtgaa ccaaatcggc ggtaaagagc gttatttctt tggcaatatt ttgtggcaga
      361 cacgggcgtt gatggaccgc gcgatcggtc ataagctggc gaaaggccgc ccggagcgcg
      421 aatatttaca gactggcgat gcggtagata gctggaaagt gattgtcgtt gaaccggaaa
      481 aacaacttac gttgttattt ggcatgaaag cgccggggct gggacgactg tgttttagcc
      541 tggaagataa aggcgactat cgtactatcg atgtccgcgc tttctggcat ccgcacggta
      601 tgccggggct gttttactgg ttattgatga tccccgcgca tctgtttatt tttcgcggaa
      661 tggcaaaaca aatcgccaga ctggcagaac aaagcacaga ttaatcgaca aaacctgcgt
      721 tattctttca tgtttcccat tgcatcatgg gggaaatcac ggaagaatgc gcacgttatt
      781 ttctcaacaa cagtggattg atatgaaggt actggttacc ggcgccacca gcggcttagg
      841 tcgaaacgcg gtagagtttt tatgccagaa aggcatcagc gtgcgagcga ccggtcgcaa
      901 cgaggcaatg ggcaaattgc tggaaaaaat gggcgcagag tttgttccgg cggatctgac
      961 cgagctggtt tcatcacaag ctaaagtgat gctcgcgggc attgatacgc tgtggcactg
     1021 ctccagcttt acctcaccct gggggacaca acaggctttc gatctggcta acgttcgcgc
     1081 cactcgccgc ctgggtgaat gggctgtcgc ctggggtgtg cgtaacttta ttcatatctc
     1141 ttccccctcc ctgtacttcg attatcacca ccatcgcgat attaaagaag attttcgccc
     1201 tcaccgcttc gccaacgagt ttgcccgcag caaagcagcc agcgaagaag tgatcaatat
     1261 gctttcgcag gcgaatccac aaacgcgctt tactattctg cgcccacaaa gtctgttcgg
     1321 accgcacgat aaagtcttta ttccccgtct ggcgcatatg atgcaccact acggcagtat
     1381 tctgttaccg catggcggca gtgcgctggt cgatatgacc tactatgaaa atgccgtgca
     1441 cgcaatgtgg ctggcaagcc aggaagcctg cgataagcta ccttccgggc gtgtgtacaa
     1501 catcaccaac ggcgagcatc gcacactgcg cagcatcgtg cagaagctga tcgacgagtt
     1561 gaatattgac tgtcgtattc gttccgtccc ctacccgatg ctggatatga tcgcccgcag
     1621 catggagcgt ttaggccgca agtcagcaaa agagccgccg ctgacccact acggcgtctc
     1681 caagttgaat tttgacttta cgctggatat tacgcgtgcg caggaagagt taggttatca
     1741 gccggtcatc accctggatg aaggtatcga gaaaactgcc gcctggctac gcgaccacgg
     1801 aaaactgccg cgctaatcct gcccgtattt ctccagcaat gcttcggcaa tcgcctgagt
     1861 ttctgcatcc gcttcgccgt tatataacgt cgggcggaaa tgcatctgga atgccataat
     1921 cacgcgccgc tgctcgcgcg gtgtcatatc aggtttaacg tcataaccgt agcgcgccaa
     1981 aagctccagc aatgacgcgg tatctaccgg tgtgtgcggc gcgcgcccgg caaggtaaaa
     2041 gttaacccgc tgcgcatccg gccaggcacc aatcccctgc tgcgccagtt gctgccaggg
     2101 aaataatggc cccggatcgt ctttgcgctg cggtgcgata tccgcatgtg ccactacgtt
     2161 ttccggcttg atgtgataac gggcaataat atctttcgcc agtggaataa gcgcctgaat
     2221 ctgtgccggt tcaaacgggg caaaatattt cactccggcc gatttttgcc agccacggtt
     2281 ttccagctca atgccaatag aggtgtcgtt aaggcgcgtt gccccgcgcc aggcgctaat
     2341 ccccgcatgc caggccagtt cttgttccgg caccagttgc cagatgcgcg gtttaccgtt
     2401 gtatcgcggt ggtaccgcag ggaccagata atgcgagctg acctgcttat cggtcagtgt
     2461 cgccagcgag ctatcaaaat catctgcggt gtagtggatc accagcactt taatgcgcgg
     2521 atacgccgcc tgcgcctggc gtcgggtatc aagctgatat ccctctttct cgacaatgcc
     2581 tttttcgcct gcacaccctg ccagtaacag agcggcagcg atcagccaga agactcttct
     2641 catcgacgcg ttttcaccgc cgtaccgctg acgctaacca tcagcatact gccgttttgc
     2701 ccgaccgttt cgtagtcgat atcaataccg acgacggcat cggcccccag cgcccgggcc
     2761 tgggagccaa gttcctcaaa ggcgatctcc cgcgctttgc gcagttcttt ttcatacgca
     2821 ccggagcgtc cgccaacgat atcgcggata ccagcaaaga aatcacggaa aatattggca
     2881 cctaaaatcg cttcgccggt caccacaccg caatattcaa cgatggtctg gccttccaga
     2941 gttggggttg tagaaaattg catgatcgat ctccttgttt gttaagcctt cgatctcaaa
     3001 agcattatcg gactgatacg ctattattga aaggatatca ttattatcgt agaacgcttt
     3061 cagagcgatc gcttataagg aaatcattat gcgctacagc aaattgacaa tgcttatccc
     3121 ctgcgcactg ctcctcagtg cctgcaccac agtcactcca gcttataaag ataacggcac
     3181 acgcagtggt ccttgcgttg aaggaggccc ggataacgtg gcccagcaat tttatgacta
     3241 ccgcattttg caccgcagca acgacatcac cgccttgcgc ccttatctga gcgacaaact
     3301 ggcgacactg cttagcgatg cctcccggga taataaccat cgggaactac tgaccaacga
     3361 tccattctcc agtcgaacca ccttgccaga cagcgcccac gttgccagtg catcgactat
     3421 ccctaatcgt gatgcccgta acattccatt gcgcgtggat ctaaaacagg gtgatcagag
     3481 ctggcaggat gaagtgttaa tgattcagga aggccagtgt tgggtgatcg acgatgtacg
     3541 ctatttaggc ggcagcgttc atgccacggc gggtacgctc cgtcagtcca tcgagaatcg
     3601 ctaacgcctt gatcaaccaa taaaccccgt aaattgtccg gcatttcgtg aggaatgccg
     3661 acatttatgc tcaccgacca ccgcccccgt tattttgtgc tatgtttatt gaataatgcg
     3721 ctttgctttt aacttttaaa gcagaaatat tgcataatta ttctgtcaaa ggtactatct
     3781 tcgacctcaa tagctatcag actgccagta tacgagtgtc aatgagtatt caattaaacg
     3841 gcattaattg cttctacggc gcgcatcagg cgctgttcga tatcacgctg gattgcccac
     3901 agggcgaaac gctggtgtta cttggcccca gcggcgcggg taaaagctcg ctgctgcgtg
     3961 tactcaatct gcttgagatg ccgcgctccg gtacgctcaa cattgcaggc aaccatttcg
     4021 attttaccaa aacaccctct gacaaagcga ttcgcgattt gcgtcgtaac gttggcatgg
     4081 tgtttcagca atacaacctg tggccgcatc tgaccgtgca gcaaaacctg attgaagcgc
     4141 cctgccgtgt actggggttg agtaaagatc aggcgctggc ccgtgcagaa aaactgctgg
     4201 aacgtctgcg tctcaaacct tatagcgatc gttacccgct gcatctttct ggtggtcagc
     4261 agcagcgtgt tgctattgcc cgtgcgttga tgatggaacc gcaggtactg ctgttcgatg
     4321 aaccgaccgc cgcactggac ccggaaatta cggcacaaat cgtcagcatc attcgtgagc
     4381 tggcagaaac gaatattacc caggtgatcg tcacccacga agttgaagtg gcgcgtaaaa
     4441 ccgccagccg agtggtgtat atggaaaatg gtcatatcgt agaacaaggc gacgcgagct
     4501 gctttaccga gccgcaaacc gaagcattta aaaactatct ctctcactaa gattcgggat
     4561 aacaacaatg aaaaaagttc tgattgccgc gttaattgca ggttttagtc tttccgccac
     4621 agctgccgaa accattcgtt ttgctaccga agcctcctat cctccgtttg aatcgattga
     4681 tgcaaacaac cagatcgttg gttttgacgt cgacctggca caggcactgt gtaaagagat
     4741 tgatgcaacc tgtactttct ctaaccaggc gtttgacagc ctgatcccaa gcctgaaatt
     4801 ccgtcgcgta gaagcagtga tggcgggcat ggatatcact ccggagcgtg aaaagcaggt
     4861 actgtttacc accccgtact atgacaactc tgccctgttt gtgggtcagc aaggcaaata
     4921 caccagtgtt gatcagctga aaggcaaaaa agtcggcgtt cagaacggga cgacacacca
     4981 gaaattcatt atggataagc acccggaaat cactaccgtt ccgtatgaca gctaccagaa
     5041 cgcaaaactg gatctgcaaa acgggcgtat cgacggcgtc ttcggtgaca ccgcagtggt
     5101 cactgagtgg ctgaaagata acccgaaact ggcggcggtg ggcgacaaag tgaccgataa
     5161 agattacttc ggcactggcc tcggcatcgc ggtacgtcag ggcaacactg agctgcagca
     5221 gaaactcaac actgcgctgg aaaaagtgaa gaaagatggc acttacgaaa ccatctacaa
     5281 caaatggttc cagaagtaat tcctgatgaa tgaatttttt cctttagcaa gcgccgccgg
     5341 gatgaccgtc ggccttgccg tttgtgcatt gattgtcggg ctggcgctgg cgatgttctt
     5401 tgcggtatgg gagtcggcaa aatggcgtcc tgtcgcgtgg gcaggttcag cgctggtaac
     5461 cattctgcgt ggcctgccag aaattctggt ggtgctgttt atctattttg gctcctcgca
     5521 gctgctgctg acgctttcgg atggcttcac tatcaatctt gggttcgtgc aaatcccagt
     5581 gcagatggac attgagaact tcgacgttag cccgttcctt tgtggtgtca tcgctctgtc
     5641 actgctgtat gccgcctatg cctcgcaaac gcttcggggc gcgttgaaag cggtgccggt
     5701 gggtcagtgg gagtctggtc aggcgctggg gctgtcgaaa tcggctatct ttttccgtct
     5761 ggtgatgccg cagatgtggc gtcatgcgct gcctggcctc ggtaaccagt ggctggtgct
     5821 gctgaaagat accgcgctgg tcagtttgat tagtgtgaat gatttaatgc tgcaaacaaa
     5881 aagcatcgct actcgtaccc aggaaccatt tacctggtac attgtggcgg cggcgattta
     5941 cctggtgatc accctgctca gtcagtacat tctcaaacgc attgacctgc gcgcgacacg
     6001 ttttgagcgg aggcccagct aatgtttgag tatttacccg aactgatgaa agggctacac
     6061 accagcctga cgctaaccgt tgcctcgctg attgtggcac tgattctggc attgattttt
     6121 accatcatcc tgacgctgaa aacgccggtg ctggtgtggc tggtgcgggg ttatatcacg
     6181 ctgtttaccg gtacgccgct gctggtgcag atcttcctga tttattacgg gccgggccag
     6241 tttccgactt tgcaggagta tccggcactg tggcatttgt tgtcagaacc gtggttatgt
     6301 gcgctgattg cgttatcgct gaacagtgcg gcgtatacca cgcagctgtt ttacggtgca
     6361 attcgtgcga tcccagaagg tcagtggcag tcctgtagcg ccctgggaat gagcaaaaaa
     6421 gatacgctgg cgatcctgct gccgtatgcc tttaaacgct cgctctcttc ttattccaac
     6481 gaagtggtgc tggtgttcaa aagtacctct ctggcttaca ccattacgct gatggaagtg
     6541 atgggataca gccagttgtt gtacggacgc acctacgatg taatggtgtt cggtgcggca
     6601 gggattattt acctggtcgt caacggcctg ctgacgctga tgatgcgtct gatcgagcgc
     6661 aaagcactgg cattcgaacg gcgaaattaa tgtagctgcg tacattcgcc gggggggttt
     6721 gtaggtctga taagacgcgc cagcgtcgca tcagacatca gcacggtgcc tgtgccggat
     6781 gcggcgtgga cgccttatcc ggcctacata gtgcataaat tcttataatg aagacggaca
     6841 actcactaag gtatccgtct ttttttattt catttaaatt atttaatcat atttattgca
     6901 tataaattca cttgatggca ttgttatccc atgccgcaga cacggccaaa aatcataaga
     6961 ttgacagacg ggagttccat catgaaaaag ttagttcttg ccgctttact tgcttccttt
     7021 actttcggtg cttctgccgc agagaaaatc aattttggcg tttcagccac ctatccaccc
     7081 tttgaatcta taggtgctaa taatgagatt gtcggctttg atatcgatct ggcaaaagcc
     7141 ttgtgcaaac aaatgcaggc agaatgtact tttactaatc acgcgttcga cagcctgatc
     7201 ccgtcactga aattcagaaa atatgacgcc gtaatctccg gtatggatat cacgcccgag
     7261 cgtagcaagc aggtatcgtt taccacgccc tactatgaaa actcagccgt cgtgattgcc
     7321 aaaaaagata cctacaaaac gtttgccgat ctgaaaggca aacgtattgg gatggaaaac
     7381 ggcaccacgc accagaaata tattcaggat cagcaccccg aagtgaaaac tgtctcctat
     7441 gacagttatc agaatgcctt tatcgatctg aaaaatggtc gtattgatgg ggtatttggt
     7501 gacacagcgg tggtaaacga atggctgaaa accaatccgc aactgggcgt tgctactgaa
     7561 aaagtgaccg atccgcaata cttcggcacc ggtctgggca tcgctgtacg tccggataac
     7621 aaagctctgc tggaaaaact gaataacgcg ctggcagcaa ttaaagctga cggtacatat
     7681 cagaaaatca atgaccagtg gtttccgcag taattgagac tgatgacaaa agtaagattg
     7741 cctgatgcgc tgcgcttatc cggcccacat caactctgca atgtattgat ttagcatgat
     7801 tttgtaggcc ggataaggcg tacacgccgc atccggcatg aacaaagcgc acctgatcag
     7861 caatctgaag ccgcacctgc ggctttttta ttgcttcacc agcagcgtca gcacttcata
     7921 gtgcgcggtg tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcggt aacctggcag
     7981 ttcgcggata tctttcgcca tggtttgggc gttacagctg gagtagataa taaaacgcgg
     8041 tgccattgtt gagagataat cacatagcgg tttaccaatg ccgcggcgcg gcggattaac
     8101 cagcaccagc tccggcacct ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg
     8161 aaattgcaaa cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc
     8221 tgatgcaatt tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc
     8281 acccacaccg cagaacagat cccacatatg ttcaactggc agctgccgta cccagtcgcg
     8341 cgcggtagcg tacagctggc tggcgaccgc cggattagtc tggaagaaac tttgcggacg
     8401 gatccacagc ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag taaggtagat
     8461 ctccgtctcc ccttccataa tcgccatatg taccggctga atattgacgg taataacttt
     8521 cagttgcggt agttgttcct gtaaccacgg cagcgcctta cgcagttgtg ccagtttggt
     8581 ttcagaacgc agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag
     8641 aatgtatttc agttcgccac gcttacgcgc cacgttgtag ggtgtcagcc ccgcacgggc
     8701 gataaacggt tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcgggcagtc
     8761 acaaaggtct tctggtgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct
     8821 accactcacc accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc
     8881 gcaccattcc tcaaccggaa aatcggcgag cagattttta agatcggcgg ttttagcgga
     8941 gagttgctct ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta
     9001 aagtgcgcac tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt
     9061 gcaactggaa gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat
     9121 ccggcagctt ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc
     9181 tgaacagctc cggataaccg taccccagcg aagccgccca caagtaactt gcggcggtga
     9241 tttgtgtcag cagatatagc cagcgcgccc agcgacgccc tttcaccagt gaaaacgcac
     9301 accagatctc aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt aaactccagg
     9361 tttgtacgct gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca
     9421 tcagcacgtc gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag
     9481 gcacattcag gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt
     9541 ccatgaaaac aatgccgcgg catgcgcggc attatgtagc caggttggca aattttagtg
     9601 tcttcagcca tgtcttgcac gctggatatc gcgtatccgc tgtttttctg cgcgagccat
     9661 cagataccag gcgataaatc cgacaattcc gaccgcaccg agaattaacg ttgcaagggc
     9721 gttgatttcc ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaacgtggt
     9781 ggctcccggc ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc
     9841 cagtaaccag ccagaaatga tcgcgggcat gatcatcggt agcgtaatga cgaaaaacac
     9901 tttcagcggc gtcgcaccga gatccatcgc tgcttcttct atcgagcgat ccagttcccg
     9961 caatcttgaa gaaataacga ccgccacata agccgtacaa aaagtgacat gcgccagcca
    10021 gatggtgagc ataccgcggt ccgcaggcca gccaatggca tgagcaagcg cgacgaataa
    10081 taacaacagc gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa
    10141 ggcaaagcca tttgatccgc gaaacctgcc aaagcgcacc agcaccaccg ccgcaatagt
    10201 cccgaggatc gccgccgccg ttgccgcaca ggcagcaatt gtcaggctta aaccaaccgc
    10261 actcatcatc gcgtcatcgc gcaataactc accataccag cgcgttgacc agccggccca
    10321 caccgtcacc agcttcgagc tgttaaacga atagataacc agcatcagca ttggcgcgta
    10381 gagaaaggta aagcccagca gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa
    10441 attattcatc cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca cattatcggc
    10501 acaattagca gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta
    10561 ttgaaaaact cttgccatag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt
    10621 tccgggatca caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc
    10681 ccacctttag tcagcggcac gatcacagta aagaacgttt tcagaggtcg tgcaccgaga
    10741 tccagcgctg cttccaccag cgaataatca atacgaatca acgcggtata aatcggcagt
    10801 accataaacg gcacgtaagc gtaaacaatg ccgatataaa cggccagatt ggtatgcaga
    10861 atggtcagcg gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg
    10921 ttttttaata ttcccatcca ggcataaacg cggatcagaa acgaggtcca cgacggcagg
    10981 atcaccagta gtaataagat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc
    11041 agcggatagc cgatcagtaa acagcaaaat gtcgaaatcg cagccacctg tagcgactgg
    11101 agataagcat cgaaatagag cggatcgtcg gtcagttgca gaaaattacc gagattaaga
    11161 gtgatggaca gttgcccgtc agcccactcc atcagttcgg tataaggtgg aatagcgcgc
    11221 gccatctccg ccaggcttat tttaaagaca atcagaaatg gcagcagaaa cagcaagatc
    11281 aaccagatat atggcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac
    11341 aaccacagct taaatccgcc cggcggtttc gactgggcag caggttcaag tgtattcatc
    11401 tttgctcctt aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg
    11461 tcgccccagg tcggtaaccc tttacgatga cgatgggcgt tttgtagctg ggcgctaatc
    11521 atctgcccac ttttcagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgt
    11581 atcacctccc ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca aagcatgatt
    11641 ttttccgggc gcagcgctac gtgtaccggc acgttatcga ccactgaagc atccgcatcg
    11701 actttcagtg gatgcaccag ccccggcgaa tcaagcacca ggccatcttc ctgacgctct
    11761 ttgagtacac cttcaaagac attaaccgaa ccgataaatt cagcgctgta gcgggtagtt
    11821 ggatgctcgt agatctcttc cggctcgcca atctggacaa atttcccgcg attcataatg
    11881 gcgatacgcc ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc
    11941 acaccgacgc gctccagaat atccaccact tcaagctgca tccggtcacg cagcttttta
    12001 tccagcgcgc ccatcggctc atcgagcagt aatagtttcg ggcgtttggc aaggcttcgg
    12061 gccagggcca cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac
    12121 tcctgcatgt gcaccagccc aagcatctcg ttgacccggg tggcaatttc cgctttcggt
    12181 agtttgtcct gtttcaggcc aaaggcgatg ttctgctcca cagtcatatg gggaaacaat
    12241 gcgtaagact gaaacatcat attgatgggg cgtagataag gtggcacctg tgacaaatcg
    12301 acgccatcaa gcataatctg tccggcagag ggttgttcaa aacctgctag catacgcagc
    12361 agcgtggatt taccacagcc ggaagcgccc agcagcgcga agatttcacc tttgtagata
    12421 gtcaggctga catcatccac cgcatgttga ccatcgtagg atttggtgag gttgcggatt
    12481 tctaatagcg gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc
    12541 acggctgctc tccggcattg aatgtgtgca agcgcaccat gatggtgcga attgccggcg
    12601 ggtgtggtgc gcccgcccgg catctaaggc tgcggattat tttccgctct tcactttggt
    12661 ccacgcgcgg gtgcgcacac ggtcgatttt cggatcctgc actttcagag tgaacagctt
    12721 cgcacggaca tccgcaggcg gataaatacc cgggttatca cggacttccg cactcaccag
    12781 cggcgtggct gctttattgg cgttggcata gaacacatgg tcggaaatat gcgctactac
    12841 atccgggcgc agcaggtaat tcaggaactg ataggcttcg tctttgtttt tggcatccgc
    12901 aggcatggcg aagacatcaa agaacgccat cgccccttct tttggaatcg agaacgagac
    12961 attaacgcca ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cgcccgccca
    13021 gccaatagcg acgcagatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa
    13081 ataacgaatg ttcgggcgca gctttaacag cagatcggta gccggtccgg tgtaatcatc
    13141 cgctttagtg ctgttgggat ctttgccgag ataattcagc acggtagcaa aaacttcttc
    13201 tggcgcatcc aggaaagaga caccgcagct tttcagtttt tccagatttt caggcttgag
    13261 gatcaagttc cagctatcga cgggcgcgtt ttcgcccagc accgctttaa ctttatcaac
    13321 gttatagcca atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc
    13381 gtgtttggcg accaacttca gcagttccgg gtcgagattc ttccactccg gcaatttgct
    13441 tttatccagc ggctggaaaa ctcccgctgt cagctggcgt tcaagaaagc ttgctgacgg
    13501 caccaccagg tcaaagccgg tgctcccggc cattaatttg ccttccagta cttcgttaga
    13561 gtcgaaaaca tcgtagacga ctttaatacc ggtttctttt tcaaaattgg ccaccgtgtc
    13621 cggggcgata taatcagacc agttataaat gtggagtgtt ttttgttcag ccgcgagcgt
    13681 gccgacagag acggccatca gagcacccgc aaccagaccc gatagccatt ttttatttaa
    13741 ggcggtcata tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact
    13801 gaaagatatg caataaacgt gcatattttt tatcggcgcg gtgtaagaaa agagaactct
    13861 ctcccggcca gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt
    13921 gagactatag ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat
    13981 gtgataaagc caaatcgaca caagagaagt gaaataatct ttaagaaaag gttaaaaaca
    14041 gcagataatt ttgcagcgcg cagccactgc accagcgact gcgtgctcga aaggagttat
    14101 cagtggagga aatagttttc ggtaacgttg ttttgccctt catcatcagt cggtaacagc
    14161 agttgatggg cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg
    14221 aaccatgcaa actgctcata ggttacgccc tgcataacgg ataaagactg gcaaaccacc
    14281 agctttggca gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca
    14341 ttgatggcag ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg
    14401 gcagaaaaca ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa
    14461 ttctgcatat ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc
    14521 aggtcatcga gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt
    14581 ttgtacaacc tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac
    14641 catgatcagt aatgtgattg cgactaacca cccgttttca ggcaatattc tgtcgtagcg
    14701 tggcgttcga tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt
    14761 ccttccagcc ccggcgacgc attcacctcc atcaccaacg gcccgcgatt agcacgcaga
    14821 atatccacac cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc
    14881 tcctgtggtg tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa
    14941 tcgccctctt tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag
    15001 cggatatcgc acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga
    15061 ccgcggaaag cgtcaatcac gctttccgcc gcctgacgcg tctccgccag cacgacgcca
    15121 attccctgcg tgccttcaac cagcttgacc accagcggcg caccaccgac catgtcgatt
    15181 aaatcgctgg tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga
    15241 cgcgccagca gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca
    15301 ttgagcggat agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta
    15361 atggcggtgc caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag
    15421 tgaatagaag acgccgcagg atttatgttc atgtagcaag aaagcggatc aagaatttca
    15481 accaggtgac cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca
    15541 tcccgggaca atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc
    15601 gcgtatcata catcgacgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa
    15661 tccagaataa atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta
    15721 tcccggcgat tattgctgcc acgggtgagg taatattccg ccagttgctc gtcatactgc
    15781 gccagtgcgc ctttatccag cggttgatag ctgttttcat gcaccaaaat ggaggccggt
    15841 aaacgcggct taagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc
    15901 agaacatgct gcggtaattt aagcagtttc gtcaccgctt caatattatt gcgcaggccg
    15961 ccgatatata ccccgcccaa tcccagcgat tccgctgcga ttaatgcatt ctgcgccatc
    16021 attgccgtat caacgacacc gagcaacagt tgttccgcca ggccgagctg agcatccgga
    16081 cagatctgta aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct
    16141 acgtgttttt gcccgccggt cagcgtcacc agttcttcac gtaacgcttt gtcggtaatg
    16201 cgaataatgc tactgcactg caaaaaactg gaactggacg tcgcacgggc gctgttaata
    16261 atcgcctcac actgcgcgtc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg
    16321 ccacaaataa gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg
    16381 cggtggacgg ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac
    16441 cgcagcggga agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg
    16501 agaaaatggc tcaggcagcg acagggagtc gcttaccgac agcagcgcca ccgccagtag
    16561 catcattccg ataaattcca gtatcaacac gcctttaggc aatttaccga tcgcgcgcat
    16621 acgcttccct ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca
    16681 gttatagctt ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag
    16741 cgtgatcatt acaggcataa atctatgagg agagaaataa tgcaaaccgt tatttttggt
    16801 cgttcgggtt gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa
    16861 cgcgatgatt ttcagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat
    16921 ctacaacaaa aggcaggtaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa
    16981 catatcggcg gctataccga ttttgctgca tgggtgaaag aaaatctgga cgcctgatcg
    17041 tctgacaagc cctcgcgttg agggctttac tgattttttc tgtgctgtgg tttaaacaaa
    17101 ctactgataa ataagaaaca cagtgccccc agcgcacacc agaacaccgc gcttagtaac
    17161 catgccagct cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat aatgagcatc
    17221 gaacagggtg ccgccagcat tgcgccaaac agaggtttca ggacttctct acgctgtgaa
    17281 aagaagctgg ccactgctcc aggaagaatg aaaaatagca agccgatttc aggatgcccg
    17341 gcagcccgaa aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg
    17401 acaaaacagc agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac
    17461 tgcgtctatc gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca
    17521 ttccatgcta atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac
    17581 tagtaaaata tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga
    17641 attctggtaa aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa
    17701 gaagttagcc tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt
    17761 attatttgtg gtcctcgcgc ttgggctatg tctcggaaag ttacgacttg gttcgatcca
    17821 actgggtaat tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag
    17881 cattaacacc gatgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga
    17941 agccggaccg aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc
    18001 actggtgatg gttggcagtg cgctggtgat cgccttaggg ttaggtaagc tgtttggctg
    18061 ggatattggc ctgacggccg gtatgttagc aggctctatg acgtcgacac cggttctggt
    18121 cggtgctggc gatacactgc gtcattccgg catggaaagc aggcagctct cactggcact
    18181 ggataatctg agcctcgggt atgccttaac ctatttaatc ggtctggtga gtttgattgt
    18241 tggtgcgcgt tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat
    18301 cgcccgcgaa cgtggcctgg acactgatgc caaccgtaag gtttatttac cggtgatccg
    18361 cgcctatcgc gtcggcccgg agctggtggc ctggaccgac ggcaaaaatc tgcgtgaact
    18421 gggtatttat cgacaaaccg gctgctacat tgaacgtatt cgacgtaacg ggattctggc
    18481 aaatccagac ggtgatgccg tgctacaaat gggcgatgaa atagcgttgg taggctatcc
    18541 cgacgcccat gcccgactcg atcccagctt ccgtaacggt aaagaagttt tcgatcgtga
    18601 ccttctcgac atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgtagg
    18661 taaacgtctc gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcattcg
    18721 tagccagatt gagatgccga tagatgacaa cgtcgtgctt aacaaaggtg acgttttaca
    18781 agtcagcggt gatgcccgtc gcgtaaaaac catcgccgat cgcatcggct ttatctcgat
    18841 tcacagccag gtcactgacc tgctggcatt ttgcgccttc tttgttattg ggctgatgat
    18901 cgggatgatc accttccagt tcagcacatt cagtttcggc atggggaacg ctgccgggtt
    18961 gttattcgcc ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat
    19021 tccgcaaggt gcattaagca tggtgaaaga gttcggcttg atggtgttta tggcaggcgt
    19081 tggtctgagc gccggtagcg gtattaataa cggcctgggc gcgattggcg gtcagatgtt
    19141 gattgccgga ttaattgtca gtcttgtgcc cgtggttatc tgtttcttgt tcggtgctta
    19201 tgtattgcga atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc
    19261 gccggcaatg gagatcatca gtgatacagc tcgcagtaac atccctgcgc tgggctatgc
    19321 gggcacctac gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt
    19381 atggccagga ttaggataaa actgaagttg ccctgaaaat gaaatttttt tgcacaaccg
    19441 cagaactttt ccgcagggca tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc
    19501 attttgtgga gcccatcaac cccgccattt cggttcaagg ttgatgggtt ttttgttatc
    19561 taaaacttat ctacaaccct gcaaccctct caaccatcct caaaatctcc tcgcgcgata
    19621 gcggcttacg gtcggtgaca aaatgcagcg tcatcccctc aataaacgca tcaagcgcgc
    19681 gggcggttcc gggttcaaac cattgttcga gcgtttgctg actgcgctgc atccagtttt
    19741 gcattaccgt ttttaatagc ggttttcggc tagccagcgc gtagagctgg tacatcagct
    19801 ccatattatc cggcgttgca acctgtgagc tgtagatcat atcggtgata gcctggcatg
    19861 cgccttgagc atcactaaca tcgctaaaaa atgcctgata ttgccgggac ataatctcag
    19921 taaaactgct gaacgcctcc agcaacaact catcaattcc tgaaaaatag taggtcatcg
    19981 atcccaacgg taccccggca agggtagcaa ttttgcggtg cgtaacagca tgtattccgt
    20041 aaagtttcac cgcctccagc gtggcctgga taattttttc tcgccgttgc ggatcgttag
    20101 cgcgacgcat gtcatttcct ctctctgcaa ttgtgtacaa atgtacacaa ccttgctaat
    20161 gttgtcttcc ctcttcttat tttgacgtga tctatgaccg taaattcttc acgtaatgca
    20221 ttgaaacgcc gaacctgggc gctgtttatg ttcttctttt tgccaggcct gttaatggcg
    20281 tcctgggcaa cccgtacgcc tgctatccgc gatattctct ctgtctcgat cgctgaaatg
    20341 ggcggtgttc tctttggtct gtcgatcggt tcgatgagcg gtattctctg ctcggcgtgg
    20401 ttagtgaaac gctttggcac gcgtaatgtc atcctggtca cgatgtcctg cgcattgatc
    20461 gggatgatga tattaagtct ggcactctgg ctgacatcgc ccctgctctt tgccgttggt
    20521 ctcggcgtct ttggggcaag ttttggttct gcggaagtgg cgataaacgt tgaaggtgct
    20581 gccgttgagc gagaaatgaa taagacggtt ttgccgatga tgcacggttt ttatagcctg
    20641 ggcacgctgg caggcgcagg tgtcgggatg gcactgacgg cctttggcgt tccggcaacg
    20701 gtgcacattt tattggcggc gctggtaggt atcgcaccta tttatatcgc cattcaggca
    20761 atccctgacg gtacgggcaa aaatgctgcc gatggcaccc agcatggcga aaaaggcgta
    20821 cctttttatc gcgatatcca gttgctgctg ataggtgttg tggtgctggc gatggccttt
    20881 gccgaaggtt ctgccaacga ctggttaccc ttattaatgg ttgacggtca cggttttagc
    20941 cctacttccg gctcgctgat ttatgccggt tttaccctgg ggatgaccgt tggacgcttc
    21001 accggcggtt ggttcatcga ccgttacagt cgcgttgccg tggttcgggc cagtgcacta
    21061 atgggggcgt tgggtattgg gctgattatt tttatcgata gcgcctgggt cgctggggtg
    21121 tctgttgtac tctggggact gggtgcctcg ctgggcttcc cgctgaccat ttctgccgcc
    21181 agcgataccg gccccgatgc accaacccgc gtcagtgtgg tagcaacgac cggttatctg
    21241 gctttcctcg tcgggccgcc gctgctgggc tatctcggcg aacattatgg attacgtagt
    21301 gcaatgctgg ttgtactggc gctggttatt ctcgcggcta ttgtcgcgaa agccgtcgcc
    21361 aaacccgata ccaaaacgca gacggcgatg gagaatagtt gatgagcatt aaattaattg
    21421 cggtagacat ggatggtact ttcttaagcg atcaaaaaac ctataaccgt gagcggttta
    21481 tggctcagta tcagcaaatg aaagcacaag gcattcgctt tgtggtcgcc agcgggaatc
    21541 aatattatca gttgatctct ttcttccctg aaattgcgaa tgaaattgcc tttgtggctg
    21601 aaaacggcgg ctgggtagtg agtgaaggca aagatgtttt taatggcgag ctgtcgaagg
    21661 atgcctttac tactgtcgtg gaacatttgc tgacgcgccc ggaagtggaa attattgcct
    21721 gcggaaaaaa tagcgcctat acgctcaaaa aatatgacga tgccatgaaa acggtggcgg
    21781 aaatgtatta tcaccgtctg gaatacgtcg ataactttga caacttagaa gatattttct
    21841 ttaagtttgg cctgaatctt tccgatgaac tgattccaca agtacaaaaa gcattacatg
    21901 aggccatcgg cgatattatg gtgccagtcc acaccggcaa cggcagcatc gatctgatta
    21961 tccccggcgt acataaagcc aatggccttc gccaactgca gaaattatgg ggaatagacg
    22021 acagcgaagt ggtggtcttt ggcgatggcg gtaacgatat tgagatgctg cgtcaggcag
    22081 gctttagttt tgcaatggaa aatgccggca gcgcggtcgt cgcagcggca aaataccggg
    22141 caggctccaa taaccgtgaa ggcgtactgg atgtgatcga taaagtgctt aaacacgaag
    22201 cgccatttga ccaataaaaa cggcagcacg cccggcaaca caatacctga atgaaggctg
    22261 cataattcgc ggaaattaac ccgctgcatt atttcaggcg aaatgattat gaaaaattgt
    22321 ctactgttgg gcgcgctttt aatgggcttt actggtgtgg cgatggcgca aagtgtcacc
    22381 gtcgatgtgc cgagtggata caaagtggtt gtggttcctg attcagtcag cgttccacaa
    22441 gcggtcagcg tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc agctccggct
    22501 taccgcccgc atccttatgt tcgccatctt gccagcgtag gtgaagggat ggttatcgaa
    22561 caccagattg acgaccatca ccattaattc gagaatgcct gatcgcacaa agcaatcagg
    22621 cattttttta cccttcgtga gaatttccca tctgtttatc ttttaaaaag ataaccatca
    22681 gcgacagcca caaaattccg ttgacaaggt tgaagagatt aaactgtccg ttgcccccgt
    22741 tcagccaggc atgtttgctg atttcaatac caacggtaaa gatcagcatt tgcagcattc
    22801 ccatcgccgc agaaaccgta cctttactca tatcgctggc aaacagggtt aatcgcacca
    22861 gtcccgcatt cgccagacca ataccgaaag cataaatact taacccggca gtcatccata
    22921 aatacgcgtg cgatgagata accgttgccg cagcagcgac caacagacca atcataatcg
    22981 gccagccgcc cataattatc agcgaacgta cggtgcggcg cgaggtcaaa cgcgctaaca
    23041 gcaagttacc cgcaattaac gccccgaaaa taggcacttg cagcaagcca tattcatagc
    23101 tgctcaactg ctcgccggta atgatgataa tcggcgactg ggcgatccac gccagcaatg
    23161 gcaggctaac gaatcccagc gccagcgccc ccgccacaaa gcggccgttc ttcagcacca
    23221 gcttatagtc acgaccgagt tctttcagtg acagtttctc gcctatacgc gtggcggttt
    23281 caggcatggc tcgttgcaga ccgaaaaagg agatcgctgc caatgcggca aacaaaacaa
    23341 acatcccctc ccagggcagc acatggatcc acgccgcgcc caccagcgga ccaagtagcg
    23401 gagcaatcag cgccacgttc gccatcagcg cggtgatctt gatacaaacc gcctcttcga
    23461 aggattcctg aattgcggcg tatcccacag cgccaatgaa acagaggctt atgccctgca
    23521 agaagcgcaa cagggtgaat tgttcaatgt tttgcgccag caatattgcc agacaggtga
    23581 tgataaacca caccaccccc gccagcatca ccggacggcg accaatacga tccgacagcg
    23641 gccccaggag ccattgtaaa aacatcccgc ccgccagata tgcggtcatt gaagtaggaa
    23701 cccaatcaat gcccgcctga tattgttcca ccacggccaa cataccgggt tgaatcatat
    23761 cgttgccgat ataggttgaa aattcgtaaa gcaccagaca gagagggaaa agtaacgcct
    23821 gacgtccaag cctggcaccg gaagctaatt tattttgcat gcaatttctt cgccaataat
    23881 aatcgcgcag agtttaataa aagcacagct aacgagaaag cgaattttgt agctgaaacc
    23941 acggttaagc acattcttac attattgcga gtatagctac gctttcttta agttttattt
    24001 aacctatgcc cgttacaatt acccaccgta aacaggccgc ttgagggaaa taagacgatg
    24061 ccgctttacc cagtttaacc tgcactttat tctcaacgac ttgcctgtat tggctccctt
    24121 ttaatcactt tgcgtcggga agttatgctg gaaaatttga atctctctct attctctctt
    24181 attaacgcga cgccagactc ggctccgtgg atgatctcgt tggcgatttt tattgctaaa
    24241 gatttgatta ccgtggtacc gctgctggcc gcggtgcttt ggttgtgggg gtttacagca
    24301 caacggcaac tggtgataaa aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg
    24361 acgatgggac atctttttcc gcacgaccga ccctttgtcg aaaatatcgg ctataacttc
    24421 ctgcatcatg cggcggatga ctcattccca agcgatcacg gtacggtgat tttcaccttt
    24481 gcactggcat ttttatgctg gcatcgcctg tggtccggct cacttttaat ggtgctggcc
    24541 gtcgtcattg cctggtcgcg cgtttatctt ggcgtccact ggccgctgga tatgctcggt
    24601 ggattgctgg caggtatgat tggctgcctt agtgcccaga ttatctggca agcgatgggg
    24661 cataaactct atcaacgtct gcaatcgtgg tatcgcgtct gttttgcatt accgatccgc
    24721 aaaggctggg tgcgtgactg atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa
    24781 taactattca gagggattat ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa
    24841 gaattaaaac gcagcgataa gttacatctt aaagacgccg ccgccctgct tggggtttcg
    24901 gagatgacga ttcgtcgcga tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc
    24961 tatattgttc tggaaccgcg cagtgccagc cattacctgt taagcgatca aaaatcccgc
    25021 ctggtggaag aaaaacgccg ggcggcaaaa ctggctgcga cactggtaga acccgatcag
    25081 accctctttt ttgactgtgg caccaccacg ccgtggatta ttgaagcgat tgataatgaa
    25141 atccctttta ccgccgtttg ttattcgcta aatacctttc tggcgctgaa agagaaaccc
    25201 cattgccgcg catttctgtg cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc
    25261 atcgattttc agcaaacgct gaataatttt tgcccggata tcgcttttta ttctgcggcg
    25321 ggcgtgcatg tcagtaaagg cgctacctgt tttaatcttg aagagttgcc ggtaaaacac
    25381 tgggccatgt cgatggcgca aaagcatgtg ctggttgtcg accacagtaa atttggcaag
    25441 gtgcgtccgg cgcgcatggg tgacctgaaa cgctttgata ttgtggtgag cgattgttgc
    25501 ccggaagatg agtatgtgaa gtacgcgcag acgcagcgca ttaagttgat gtattaatga
    25561 cgtataaccg gatgacgttt cgcgccatcc ggttatcaga agattaagag aaccagctgc
    25621 cgaaccactg atggaatttc atcatcacga aatcccacat ccgaccaaag aatccgccct
    25681 cttccacatt ttccatcacg atcagcggac gctgctcaat ggatttaccg ttaagctgga
    25741 aatcaatggt cccgacaacc tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta
    25801 acgtataact cgctttcagg tttttcagct gcccacgcgg tatggtcact gagcccgctt
    25861 cacctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga gtcacaaagg
    25921 tggcatcagg tttaattggc gtcacggttt caaagaagcg gaaaccccag gtcaataatt
    25981 tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg gagattaaac
    26041 gcatatcgcc ctgggtagcc gaagcaacca gattatatcc cgcgcctgcc gtggttcctg
    26101 tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg ttaggctgac
    26161 gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc ggcacatcgt
    26221 ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc cccggcgcat
    26281 ccagaccgtg caccgtctgg aaggtagtgt tggtcagacc cagttttttg gcataaccat
    26341 tcatcaaacc aatgaatgac tcctggctcc cggcaacgta atcggccagc gcaatacagg
    26401 cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa acctgatcgc
    26461 ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc gcccaggcat
    26521 ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc gcctgcccaa
    26581 ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc cagtttctca tccgcgttgc
    26641 cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc
    26701 tcggcgcttc aacggtttgt tccgccgcga atgccgttgg ggcaaaaagg aataaaaatg
    26761 cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat gccacccgag
    26821 tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat taataacgca
    26881 cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc aatatcctgc
    26941 cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt aactttactt
    27001 caccatcgga gtcctgttca gcctgtggct gcctgtcgga aaaggggtga gtatgattac
    27061 gctgtggggt cggaataatt caaccaacgt aaaaaaagta ttgctgacgc tcgaagaact
    27121 ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc acgatgctga
    27181 ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg aaagtgatct
    27241 cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg ggcaaaaacg
    27301 tctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg actgggcaaa
    27361 ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa caccaccgga
    27421 agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc tgtttgccct
    27481 gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg gtgtgggcga
    27541 tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctcacct ggacaccgcg
    27601 tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc gcaaagtggt
    27661 gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac tggactcgtc
    27721 ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc gctgccctct
    27781 gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca tcacaatgac
    27841 atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga atttatcgct
    27901 gttatagaag gccatgccgc tcacggcggg cgaatctttc cagtaaaaaa caggttgctc
    27961 ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat agttgattcc
    28021 ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa tttcatcacc
    28081 accgcgcggg ccatgttcat tcagccatag tgcattactc cacggattca tcgccattcc
    28141 ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt cctttataaa
    28201 aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc cctgtaattt
    28261 atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaacaa aaagataacc
    28321 tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt ttggcatctg
    28381 gcgaaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc ggccataacc
    28441 cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc agatgcggcg
    28501 agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc cgtgcgccca
    28561 aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc agtgacgcaa
    28621 ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg ccagtgccca
    28681 gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag ccgcccagag
    28741 agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca taacgcctcc
    28801 ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat ttttacaaaa
    28861 acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt ctcaagcatg
    28921 gcgtgcttct gcaggctctg gatactcagc gttaagctca tcagacaatt ttcaagctta
    28981 tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa acatactgcg
    29041 gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg atccagatga
    29101 agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa gttatctcgt
    29161 agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg cacgtatgca
    29221 gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg tctgtcaacg
    29281 aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg gctatttttt
    29341 gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt acatttcctt
    29401 cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg tagaatccct
    29461 gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa ctccccagcc
    29521 gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag agcgtattct
    29581 caccgaacta cgcactgaag gttatgacgt ggtaccgagc tatgacgatg cggacatggt
    29641 gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg aagccattgg
    29701 tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg caaaagaaga
    29761 tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata gctatgagca
    29821 ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat tcctgagcct
    29881 ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga aaatttcgga
    29941 aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg acctggtgag
    30001 ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg gcgttaaaga
    30061 gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac atcgtactgg
    30121 cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac agttatcgaa
    30181 actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg acgacgtcat
    30241 cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc agcacgccag
    30301 cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac tggcgcgcat
    30361 caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta ttgtcggctt
    30421 ccctggcgag acagaagaag atttccagat gctactagac ttcctgaaag aagcgcgtct
    30481 ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca atgccctgcc
    30541 tgaccaggtt ccggaagaag ttaaagaaga acgctggaac cgtttcatgc agctgcagca
    30601 gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg tgattatcga
    30661 cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg aaatcgacgg
    30721 cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc gtgtgaaagt
    30781 cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc aggccgggca
    30841 aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgagc cctgccgttt
    30901 ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac ggtggtacat
    30961 tcatttgaat gcgctacacc gatactggta cttaaattaa tgcttcccac ctggaaatga
    31021 acgatttctt ttagaatcga tagcaactct tccagcgttc cacccgtata aatcaccgca
    31081 aatttgtcac cgccgaagcg gtagatttta cctttatcta ccgggatctg tgatttaagg
    31141 attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt gtcgttaatg
    31201 tttttgaatt tatcgacgtc aaacaggcac aaataaccgc ctttttccgg cagcgctttt
    31261 aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag gaacgccgag
    31321 ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc gccatccagt
    31381 acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg attaatcact
    31441 ctgcgtacca gatagaggca actggtaccc gatatcactg ccaccaatgc ggcaaagaaa
    31501 ataatcaatg tacagacccg caaataaaca tcttttttat acgcaaatac gtacaatccg
    31561 gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc gtttttagca
    31621 ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat caggttctcg
    31681 ctgtccatcg ccagcgattt tgacagtaaa gaaaaacgct ccagaccaag caaaaatatc
    31741 agactacgat tgtgagttac cggatagagg taatagacga aggttttacc gtcaaaagag
    31801 ataaaccctg tatttttgac atgtcgatac ttcacaacct gcataatatc tgcacaataa
    31861 tttttctcga actcctttac ctgaatacat tcgtcactgg aaatcagata aggaccgaca
    31921 tcgtacaaat aagaggggga aaccgagtcg gcgtaagctg tgcgcaaacc aaccaacagc
    31981 tgttcctccg tgggatttag cgtgttgata agatcaattg ccacgctacg ccgatattca
    32041 gcaatatttg ccaccgcaaa agcagtatca ataacggact gtttcaggct atacacccgt
    32101 tcctttacca tttgagtgta gatcccgcag gcaactgctg aaatcatgat aaaaatcagc
    32161 agactgactg taagtacgaa cttattgatt ctggacatac gtaaactact cttttactaa
    32221 ttttccactt ttatcccagg cggagaatgg catgggtttc tggtacagat acccctgagc
    32281 ctgaacacat cctaatttaa ttaattcgcg cgcaacgtct tcgctctcta cgccttccgc
    32341 aatcaccggg atccggttat agcgtgccag atcgaaaata atgcgcacaa tttttcccgc
    32401 agtaccgaaa atatcgccgg taaattgcga gtccaccttt atgctgtctg gattaatttc
    32461 ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca tcaatcgata tccgtacccc
    32521 ttgtttacgc agttgttcaa tattgagata aaaatgttca ttaattcgct cgatcacggt
    32581 ttcagtgatc tcaacacaga ggctgttcgc cttcaggcca ttttctttca tgatggtggt
    32641 aaaacgctca tgaaagtgcg gttcgtttaa ctgccagggc gaaacattaa tatgcagtaa
    32701 gaaatcatcc ttcagcgacg cacgctgact aaaatgtctg aattcaccca ttgccgtttt
    32761 aataatctga taacccagct cattgataaa cccgatatct tcagcaatgg taatgaactt
    32821 cagcggtgaa atgatccccc gctccgcaga aacccaacgg cacaacgcct ccgcgcccag
    32881 aatggcgcgg gtgttaatat caacgatcgg ttgatagacg aggaatagtt cgccaatttc
    32941 cattgcatgg cgaatattac gcgcaatcca gatattctct tctatttcgt tgagtttcgc
    33001 gtcatcacag aaaataaact cggagaacat atggtcacgg atactggaaa tcgccagttt
    33061 cagtttttct gcgtaactcc aggcgttttc cccctgcaaa ggttcacaaa tggcagactt
    33121 atggatgcgc agattgcggt agccaaagct gctgtcaaaa tcgtttacca tcgctatttt
    33181 acgggtaaat gtctgtacat tttcccgaca cagaagtaaa tagagatcgg cattgaatgt
    33241 cacgatatcg cagcattgcg catatttctc ggcaattcgc tgacacaatt tcctgacgat
    33301 tttattgcgt tcatcaacac caaacaccgc ttccacggct tccagaccac acagtttgat
    33361 gatccccaca gccagatact gattattgtt ggactcaata atcgccatat ttttaataaa
    33421 accgctttcc tgatcgtaag agatcagtga gagcaattta tttgaccgtt caatttcggc
    33481 atctttcatt tccgcgatcg aatttttgat gctgataatc tctttcgcat cgccttcgcg
    33541 aattttcaga gcctcgattt gatccgttga gtcgggcaaa aacgccagcg tattaaccag
    33601 ctcggtcatt tcagaaaatt gccgacggaa cacacgagtg gtcatgaaat ataccaccag
    33661 caacacgata ataatgaaag caaacggtag ataaaaaagt ttatctgatg cgttcaataa
    33721 attaccgcca ggataataaa aatagatttc gaacaatgca ttatttggca ctgctgatgt
    33781 ctttgcaata tactccgtat cgacagaatc aataatatta taattacctt cgctattata
    33841 aataatcagt ttttcacgag cgagttgatt cttggcaatg atctcatgac cttgtttcaa
    33901 aaatagaact tcatcattag cgctgatttg atcaagatac tcgttcagat accgccagtt
    33961 taaatcgaca tgaattttat ctaccgctaa caaatcgtga tctttgccgt tgatacgtag
    34021 cgtaaaagag atacattttt tacccggacg atcaaagcaa ttgtatgccg gattccagct
    34081 gttgttagag tcatctgaag ccaccgacca cggtcgtttc gatgggttaa aatcggcaaa
    34141 aataacccgc cctgactcgt cgacaagcgg tccatttact tgttcctgat gataaacaac
    34201 gattttatca ttcgccctcc gcgaaaagag ataata
//
LOCUS       DAZFVO020000037        34130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFVO020000037 DAZFVO020000000
VERSION     DAZFVO020000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 34130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.037"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHKNB_RS08330"
                     /old_locus_tag="pgaptmp_001666"
     CDS             complement(158..370)
                     /gene="rpmE"
                     /locus_tag="ACHKNB_RS08330"
                     /old_locus_tag="pgaptmp_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HGU4195199.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            573..2771
                     /gene="priA"
                     /locus_tag="ACHKNB_RS08335"
                     /old_locus_tag="pgaptmp_001667"
     CDS             573..2771
                     /gene="priA"
                     /locus_tag="ACHKNB_RS08335"
                     /old_locus_tag="pgaptmp_001667"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGU4195200.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2927..3952
                     /gene="cytR"
                     /locus_tag="ACHKNB_RS08340"
                     /old_locus_tag="pgaptmp_001668"
     CDS             2927..3952
                     /gene="cytR"
                     /locus_tag="ACHKNB_RS08340"
                     /old_locus_tag="pgaptmp_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HGU4195201.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHKNB_RS08345"
                     /old_locus_tag="pgaptmp_001669"
     CDS             4044..5003
                     /gene="ftsN"
                     /locus_tag="ACHKNB_RS08345"
                     /old_locus_tag="pgaptmp_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HGU4195202.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5096..5626
                     /gene="hslV"
                     /locus_tag="ACHKNB_RS08350"
                     /old_locus_tag="pgaptmp_001670"
     CDS             5096..5626
                     /gene="hslV"
                     /locus_tag="ACHKNB_RS08350"
                     /old_locus_tag="pgaptmp_001670"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HGU4195203.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5636..6967
                     /gene="hslU"
                     /locus_tag="ACHKNB_RS08355"
                     /old_locus_tag="pgaptmp_001671"
     CDS             5636..6967
                     /gene="hslU"
                     /locus_tag="ACHKNB_RS08355"
                     /old_locus_tag="pgaptmp_001671"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HGU4195204.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7034..7960
                     /gene="menA"
                     /locus_tag="ACHKNB_RS08360"
                     /old_locus_tag="pgaptmp_001672"
     CDS             7034..7960
                     /gene="menA"
                     /locus_tag="ACHKNB_RS08360"
                     /old_locus_tag="pgaptmp_001672"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HGU4195205.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8053..8538
                     /gene="rraA"
                     /locus_tag="ACHKNB_RS08365"
                     /old_locus_tag="pgaptmp_001673"
     CDS             8053..8538
                     /gene="rraA"
                     /locus_tag="ACHKNB_RS08365"
                     /old_locus_tag="pgaptmp_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HGU4195206.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="ACHKNB_RS08370"
                     /old_locus_tag="pgaptmp_001674"
     CDS             complement(8623..8868)
                     /gene="zapB"
                     /locus_tag="ACHKNB_RS08370"
                     /old_locus_tag="pgaptmp_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HGU4195207.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9293..10138
                     /gene="glpF"
                     /locus_tag="ACHKNB_RS08375"
                     /old_locus_tag="pgaptmp_001675"
     CDS             9293..10138
                     /gene="glpF"
                     /locus_tag="ACHKNB_RS08375"
                     /old_locus_tag="pgaptmp_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HGU4195208.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10161..11669
                     /gene="glpK"
                     /locus_tag="ACHKNB_RS08380"
                     /old_locus_tag="pgaptmp_001676"
     CDS             10161..11669
                     /gene="glpK"
                     /locus_tag="ACHKNB_RS08380"
                     /old_locus_tag="pgaptmp_001676"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGU4195209.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11804..12814
                     /gene="glpX"
                     /locus_tag="ACHKNB_RS08385"
                     /old_locus_tag="pgaptmp_001677"
     CDS             11804..12814
                     /gene="glpX"
                     /locus_tag="ACHKNB_RS08385"
                     /old_locus_tag="pgaptmp_001677"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HGU4195210.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12911..13657
                     /gene="fpr"
                     /locus_tag="ACHKNB_RS08390"
                     /old_locus_tag="pgaptmp_001678"
     CDS             12911..13657
                     /gene="fpr"
                     /locus_tag="ACHKNB_RS08390"
                     /old_locus_tag="pgaptmp_001678"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HGU4195211.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHKNB_RS08395"
                     /old_locus_tag="pgaptmp_001679"
     CDS             complement(13662..14090)
                     /gene="uspD"
                     /locus_tag="ACHKNB_RS08395"
                     /old_locus_tag="pgaptmp_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HGU4195212.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14117..14416)
                     /gene="yiiS"
                     /locus_tag="ACHKNB_RS08400"
                     /old_locus_tag="pgaptmp_001680"
     CDS             complement(14117..14416)
                     /gene="yiiS"
                     /locus_tag="ACHKNB_RS08400"
                     /old_locus_tag="pgaptmp_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HGU4195213.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14628..15068)
                     /gene="yiiR"
                     /locus_tag="ACHKNB_RS08405"
                     /old_locus_tag="pgaptmp_001681"
     CDS             complement(14628..15068)
                     /gene="yiiR"
                     /locus_tag="ACHKNB_RS08405"
                     /old_locus_tag="pgaptmp_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4195214.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15169..15768
                     /gene="yiiQ"
                     /locus_tag="ACHKNB_RS08410"
                     /old_locus_tag="pgaptmp_001682"
     CDS             15169..15768
                     /gene="yiiQ"
                     /locus_tag="ACHKNB_RS08410"
                     /old_locus_tag="pgaptmp_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HGU4195215.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHKNB_RS08415"
                     /old_locus_tag="pgaptmp_001683"
     CDS             15876..16643
                     /gene="tpiA"
                     /locus_tag="ACHKNB_RS08415"
                     /old_locus_tag="pgaptmp_001683"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGU4195216.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHKNB_RS08420"
                     /old_locus_tag="pgaptmp_001684"
     CDS             complement(16698..17453)
                     /gene="cdh"
                     /locus_tag="ACHKNB_RS08420"
                     /old_locus_tag="pgaptmp_001684"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HGU4195217.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHKNB_RS08425"
                     /old_locus_tag="pgaptmp_001685"
     CDS             complement(17560..18549)
                     /gene="sbp"
                     /locus_tag="ACHKNB_RS08425"
                     /old_locus_tag="pgaptmp_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HGU4195218.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGMFDQISKR"
     gene            complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="ACHKNB_RS08430"
                     /old_locus_tag="pgaptmp_001686"
     CDS             complement(18869..19831)
                     /gene="pfkA"
                     /locus_tag="ACHKNB_RS08430"
                     /old_locus_tag="pgaptmp_001686"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGU4195219.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="ACHKNB_RS08435"
                     /old_locus_tag="pgaptmp_001687"
     CDS             complement(20012..20914)
                     /gene="fieF"
                     /locus_tag="ACHKNB_RS08435"
                     /old_locus_tag="pgaptmp_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HGU4195220.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21063..21563)
                     /gene="cpxP"
                     /locus_tag="ACHKNB_RS08440"
                     /old_locus_tag="pgaptmp_001688"
     CDS             complement(21063..21563)
                     /gene="cpxP"
                     /locus_tag="ACHKNB_RS08440"
                     /old_locus_tag="pgaptmp_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HGU4195221.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21713..22411
                     /gene="cpxR"
                     /locus_tag="ACHKNB_RS08445"
                     /old_locus_tag="pgaptmp_001689"
     CDS             21713..22411
                     /gene="cpxR"
                     /locus_tag="ACHKNB_RS08445"
                     /old_locus_tag="pgaptmp_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HGU4195222.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22408..23781
                     /gene="cpxA"
                     /locus_tag="ACHKNB_RS08450"
                     /old_locus_tag="pgaptmp_001690"
     CDS             22408..23781
                     /gene="cpxA"
                     /locus_tag="ACHKNB_RS08450"
                     /old_locus_tag="pgaptmp_001690"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HGU4195223.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23887..24561)
                     /gene="yiiM"
                     /locus_tag="ACHKNB_RS08455"
                     /old_locus_tag="pgaptmp_001691"
     CDS             complement(23887..24561)
                     /gene="yiiM"
                     /locus_tag="ACHKNB_RS08455"
                     /old_locus_tag="pgaptmp_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HGU4195224.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24710..25693)
                     /gene="kdgT"
                     /locus_tag="ACHKNB_RS08460"
                     /old_locus_tag="pgaptmp_001692"
     CDS             complement(24710..25693)
                     /gene="kdgT"
                     /locus_tag="ACHKNB_RS08460"
                     /old_locus_tag="pgaptmp_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HGU4195225.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25953..26573)
                     /gene="sodA"
                     /locus_tag="ACHKNB_RS08465"
                     /old_locus_tag="pgaptmp_001693"
     CDS             complement(25953..26573)
                     /gene="sodA"
                     /locus_tag="ACHKNB_RS08465"
                     /old_locus_tag="pgaptmp_001693"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HGU4195226.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26858..27892
                     /gene="rhaT"
                     /locus_tag="ACHKNB_RS08470"
                     /old_locus_tag="pgaptmp_001694"
     CDS             26858..27892
                     /gene="rhaT"
                     /locus_tag="ACHKNB_RS08470"
                     /old_locus_tag="pgaptmp_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HGU4195227.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGIAN"
     gene            complement(27889..28737)
                     /gene="rhaR"
                     /locus_tag="ACHKNB_RS08475"
                     /old_locus_tag="pgaptmp_001695"
     CDS             complement(27889..28737)
                     /gene="rhaR"
                     /locus_tag="ACHKNB_RS08475"
                     /old_locus_tag="pgaptmp_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HGU4195228.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28811..29647)
                     /gene="rhaS"
                     /locus_tag="ACHKNB_RS08480"
                     /old_locus_tag="pgaptmp_001696"
     CDS             complement(28811..29647)
                     /gene="rhaS"
                     /locus_tag="ACHKNB_RS08480"
                     /old_locus_tag="pgaptmp_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HGU4195229.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29935..31404
                     /gene="rhaB"
                     /locus_tag="ACHKNB_RS08485"
                     /old_locus_tag="pgaptmp_001697"
     CDS             29935..31404
                     /gene="rhaB"
                     /locus_tag="ACHKNB_RS08485"
                     /old_locus_tag="pgaptmp_001697"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HGU4195230.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFIPNPDSEIAHYLAQIHSTRQTKELCA"
     gene            31401..32660
                     /gene="rhaA"
                     /locus_tag="ACHKNB_RS08490"
                     /old_locus_tag="pgaptmp_001698"
     CDS             31401..32660
                     /gene="rhaA"
                     /locus_tag="ACHKNB_RS08490"
                     /old_locus_tag="pgaptmp_001698"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HGU4195231.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            32902..33726
                     /gene="rhaD"
                     /locus_tag="ACHKNB_RS08495"
                     /old_locus_tag="pgaptmp_001699"
     CDS             32902..33726
                     /gene="rhaD"
                     /locus_tag="ACHKNB_RS08495"
                     /old_locus_tag="pgaptmp_001699"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HGU4195232.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33736..34050
                     /gene="rhaM"
                     /locus_tag="ACHKNB_RS08500"
                     /old_locus_tag="pgaptmp_001700"
     CDS             33736..34050
                     /gene="rhaM"
                     /locus_tag="ACHKNB_RS08500"
                     /old_locus_tag="pgaptmp_001700"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HGU4195233.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8343 a         8892 c         8862 g         8033 t
ORIGIN      
        1 taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca acaggaaatg
       61 ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta caggcacaaa
      121 aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc gggatgttga
      181 aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt ttgccagtga
      241 agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg gtggagcgga
      301 ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc gggtgaatat
      361 cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc taacgccaga
      421 caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc
      481 atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca atgtgtatac
      541 taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt gccttgcccg
      601 ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt aaagccgggt
      661 gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta
      721 gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt agtggaagtg ctggacgttg
      781 agccggtatt tactcactcc gtctggcggt tactgctctg ggcggcagat tactatcatc
      841 atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag gggcggcctg
      901 cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccaggcg gtggatctga
      961 acagcctgaa acgctccccc aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa
     1021 tctggcgcga ccaggtcgcc acgctcgaat ttaatgatgc cgcgttgcag gcgctacgca
     1081 aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg cgaacgaact
     1141 atgccgtttc tggtgagcgg ttgcgattga ataccgaaca agccaccgcc gttggcgcaa
     1201 ttcatagcgc ggcagatact ttttctgcct ggctgctggc gggcgttacc ggttccggta
     1261 aaacggaggt ttatctcagt gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg
     1321 tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta
     1381 atgcccccgt ggaagttctg cattccggcc tgaacgacag cgagcggctt tcggcgtggt
     1441 tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc
     1501 cgtttaaaaa tctcggcgtg attgtcattg atgaagagca cgacagctcc tacaagcagc
     1561 aggaaggctg gcgctatcat gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa
     1621 tcccgattat tcttggctcc gcaacgcccg cgctggaaac gttatgcaac gtccagcaga
     1681 aaaaataccg cctgctgcgc ctgacccgtc gggcagggaa tgcgcgtccg gcaattcaac
     1741 atgtgctgga tttaaaaggt cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc
     1801 gtatgcgcca gcatttacag gccaacaatc aggttatcct ctttcttaat cgtcggggtt
     1861 ttgcgcctgc gctgctgtgc cacgactgcg gctggatagc cgaatgccca cgttgcgatc
     1921 actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt gacagtcagc
     1981 gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtcccc gtggggctgg
     2041 gcaccgaaca gcttgaacag acgcttgcgc cgatgttccc cggcgtgccc atttcacgta
     2101 tcgaccgcga taccaccagc cgcaaagggg cgctggaaca gcaactggcg gaagtgcatc
     2161 gcggcggcgc gaggatcttg attggcacgc aaatgctggc gaaaggtcac cactttccgg
     2221 atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct gttttctgcc gattttcgct
     2281 cggcagaacg tttcgcccag ctttacaccc aggtcgccgg tcgtgccggg cgtgcgggta
     2341 aacagggcga agtggtgctg caaacgcacc atccggaaca tcctctgttg caaacgttgc
     2401 tctataaagg ctacgacgcc tttgccgaac aggcgctggc tgagcgacgc atgatgcagc
     2461 tgccgccgtg gaccagccat gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc
     2521 cgctgttcct gcaacaactg cgtaatctga tcctttccag cccgctggca gacgacaaac
     2581 tgtgggttct cggcccggtt ccggctctgg cacctaagcg tggtggccgc tggcgctggc
     2641 agatcttgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg
     2701 cgcttattaa tacaataccg gattcccgca aggtgaaatg ggtgttggat gtcgatccca
     2761 tcgaaggcta atgcgcaaac gttgcgaggc ggatcgaaaa attcaatatt catcacactt
     2821 ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa
     2881 gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa
     2941 gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac
     3001 tgtctcccga gcattaatga atcccgataa agtctcccag gccacccgta atcgggttga
     3061 aaaagcggcc cgggaagtgg gttatttacc gcaacctatg gggcgcaacg tcaagcgtaa
     3121 tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct ttagcgaaat
     3181 tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga ttggcgactg
     3241 tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca agcaaattga
     3301 tggcatgttg ctgctgggct caaggctgcc gtttgatgcc agcattgagg aacagcgtaa
     3361 tctgccgccg atggtgatgg cgaacgaatt tgcaccggaa ctggagctgc ctaccgttca
     3421 tatcgacaat ctgaccgccg catttgatgc agtaaattat ttatatgagc aagggcataa
     3481 acggattggc tgtatagccg gtcccgaaga gatgccactg tgtcactacc gcctgcaagg
     3541 ctatgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca tcgcccgtgg
     3601 cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc ttccacaacc
     3661 gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa
     3721 acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata acatcgacct
     3781 gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg
     3841 ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca gtggctctcg
     3901 tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt aaagtaaacc
     3961 tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg ggctgacgaa
     4021 cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc agccaaccgg
     4081 caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg cctgcggttt
     4141 ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt ggtctgtact
     4201 tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa gtgaccggta
     4261 acggactacc accaaaacca gaagaacgct ggcgctacat taaagagctg gaaagtcgcc
     4321 agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa acgccggagc
     4381 aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg cgccagcagc
     4441 caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc
     4501 tacaacgcca gcgtcaggcg cagcaattag cggagcagca acgcctggct cagcagtcgc
     4561 gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg
     4621 cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa
     4681 cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg
     4741 ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg
     4801 gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg ttcgaaggat
     4861 ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag
     4921 gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat acaaactgca
     4981 ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa ttgcattatg
     5041 ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc agctcgtgac
     5101 aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg gtcaggccac
     5161 gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa
     5221 agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg aactgtttga
     5281 acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc tggcgaaaga
     5341 ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac
     5401 tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg atcttattgc
     5461 tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag aaaatactga
     5521 actgagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta
     5581 taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc
     5641 tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg gccaggacaa
     5701 cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga
     5761 agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg
     5821 taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca tcaaagttga
     5881 agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta ttattcgcga
     5941 tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa accgttatcg
     6001 cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg ctaaaaacaa
     6061 ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa
     6121 actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg cagcaccgat
     6181 gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc tgcagtccat
     6241 gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat
     6301 gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc tgaagcaaga
     6361 cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg acaaaatctg
     6421 taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct
     6481 gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa ctgaccacat
     6541 tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga tcccggaact
     6601 gcaaggtcgt ctgccaatcc gcgttgaact gcaggcgctg accaccagcg acttcgagcg
     6661 tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga tggcgactga
     6721 aggcgtaaat atcgagttta ccgactccgg tattaagcgc atcgcggaag cggcatggca
     6781 ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc tggagcgttt
     6841 aatggaagag atttcctacg acgccagcga tttaagcggt caaactatca tcattgacgc
     6901 agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga gccgttttat
     6961 cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg ccccattttt
     7021 attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc tggaaagttt
     7081 acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacag cgctggcatg
     7141 gtggcaaggt cacttcgatc cgctggtcgc cctgctggca ctgattaccg ccgggctatt
     7201 acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc
     7261 tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc aagagatgaa
     7321 acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac tggttgcagt
     7381 ggcatgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt tgtcgatcat
     7441 tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc tgggtgatat
     7501 ttccgtactg gttttctttg gctggttgag tgtcatgggg agctggtatt tacaggctca
     7561 tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg caacggcagt
     7621 actgaatatt aataacctgc gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct
     7681 ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc
     7741 gctggtgtgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg gctggctgtt
     7801 cctgctggcg gcaccattac tggtgaagca agcccgttat gtgatgcggg aaatggaccc
     7861 ggtggcgatg cgaccaatgc tggaacgtac tgtcaaggga gcgttactga ctaacctgct
     7921 gtttgtttta gggatattcc taagccagtg ggcagcataa ctgacaaata tcaattaaca
     7981 attgatgatt ttgccaacag cctacatagc gcgatatact gaaaattctc gcagcaactg
     8041 aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca agaagatgtt
     8101 aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg cggacaaata
     8161 atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga acagaatggc
     8221 cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct
     8281 gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg
     8341 cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc ggcaattccg
     8401 gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc
     8461 accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct ttctgaagat
     8521 ccgctggata ttgaatgata gaaaaggcac cgcagggtgc cttttttctg cgttacctgt
     8581 tggcctacac agtaaagaaa ttacgcggaa gatgaagcgt aatcagacct cttccatgcg
     8641 acccagcagg gcctgcagac gttcctgcca gccgttctgc tgttctttca gatggttgtt
     8701 ctcacgctcc agctcttcgc gctgatgctg ggcattttga acttcctgcg acagtgagtt
     8761 gtttttttct ttcagctctt cgatttccat ctgcaacaga gtgatggtat caatcgcctg
     8821 ctgtactttt gcttccagtt tctcaaacac ttctaatgac attgtcatac ctctcctgaa
     8881 ttgcaaggcg ttgatggata aaaatcctcg tcccgattac cggtgacgcc ttaataaata
     8941 cgagcgcact ttagttagct ccgattgtat gaagccgcgc catcgctgtc cagcggcacg
     9001 ccttgcagat tacggtttgc cacacttttc atccttctcc tggtgacata atctacatca
     9061 atcgaaaatg ttaataaatt tgttgcgcga atgatctaac aaacatgcat catgtacaat
     9121 cagatggaat aaatggcgcg ataacgctca ttttatgacg aagcacacac attttaagtt
     9181 cgatatttct cgtttttgct cgttaacgat aagtttacag catgcctaca agcatcgtgg
     9241 aggtccgtga ctttcacgca tacaacaaac attaactctt caggatccga ttatgagtca
     9301 aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc ggtaccgggt tgttgatttt
     9361 cttcggtgtg ggttgcgttg cagcactaaa agtcgctggt gcgtcttttg gtcagtggga
     9421 aatcagtgtc atttggggac tgggggtggc aatggccatc tacctgaccg caggggtttc
     9481 cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg ctgtttgcct gtttcgacaa
     9541 gcgcaaagtt attcctttta tcgtttcaca agttgccggc gctttctgtg ctgcggcttt
     9601 agtttacggg ctttactaca atttattttt cgacttcgag cagactcatc acattgttcg
     9661 cggcagcgtt gaaagtgttg atctggctgg cactttctct acttacccta atcctcatat
     9721 caattttgtg caggctttcg cagttgagat ggtgattacc gctattctga tggggctgat
     9781 cctggcgtta acggacgatg gcaacggtgt accacgcggc cctttggctc ccttgctgat
     9841 tggtctactg attgcggtca ttggcgcatc tatgggccca ttgacaggtt ttgccatgaa
     9901 cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg gcgggctggg gcaatgtcgc
     9961 ctttaccggc ggcagagaca ttccttactt cctggtgccg cttttcggcc ctatcgttgg
    10021 cgcgattgta ggtgcatttg cctaccgcaa actgattggt cgccatttgc cttgcgatat
    10081 ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa caaaaagctt cgctgtaata
    10141 tgactacggg acaattaaac atgactgaaa aaaaatatat cgttgcgctc gaccagggca
    10201 ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa tatcattagc gtgtcgcagc
    10261 gcgaatttga gcaaatctac ccaaaaccag gttgggtaga acacgaccca atggaaatct
    10321 gggccaccca aagctccacg ctggtagaag tgctggcgaa agccgatatc agttccgatc
    10381 aaattgcagc tatcggtatt acgaaccagc gtgaaaccac tattgtctgg gaaaaagaaa
    10441 ccggcaagcc tatctataac gccattgtct ggcagtgccg tcgtaccgca gaaatctgcg
    10501 agcatttaaa acgtgacggt ttagaagatt atatccgcag caataccggt ctggtgattg
    10561 acccgtactt ttctggcacc aaagtgaagt ggatcctcga ccatgtggaa ggctctcgcg
    10621 agcgtgcacg tcgtggtgaa ttgctgtttg gtacggttga tacgtggctt atctggaaaa
    10681 tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc ctctcgtacc atgttgttca
    10741 acatccatac cctggactgg gacgacaaaa tgctggaagt gctggatatt ccgcgcgaga
    10801 tgctgccaga agtgcgtcgt tcttccgaag tatacggtca gactaacatt ggcggcaaag
    10861 gcggcacgcg tattccaatc tccgggatcg ccggtgacca gcaggccgcg ctgtttggtc
    10921 agttgtgcgt gaaagaaggg atggcgaaga acacctatgg cactggctgc tttatgctga
    10981 tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct gctgaccacc atcgcctgcg
    11041 gcccgactgg cgaagtgaac tatgcgttgg aaggtgcggt gtttatggca ggcgcatcca
    11101 ttcagtggct gcgcgatgag atgaagttga ttaacgacgc ctacgattcc gaatatttcg
    11161 ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc ggcatttacc gggctgggtg
    11221 cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg tctgactcgt ggggtgaacg
    11281 ctaaccacat tatccgcgcg acgctggagt ctattgctta tcagacgcgt gacgtgctgg
    11341 aagcgatgca ggccgactct ggtatccgtc tgcacgccct gcgcgtggat ggtggcgcag
    11401 tagcaaacaa tttcctgatg cagttccagt ccgatattct cggcacccgc gttgagcgcc
    11461 cggaagtgcg cgaagtcacc gcattgggtg cggcctatct cgcaggcctg gcggttggct
    11521 tctggcagaa cctcgacgag ctgcaagaga aagcggtgat tgagcgcgag ttccgtccag
    11581 gcatcgaaac cactgagcgt aattaccgtt acgcaggctg gaaaaaagcg gttaaacgcg
    11641 cgatggcgtg ggaagaacac gacgaataat gtaaatgccg aatgaagcgt ttatgccgca
    11701 tccggtagtc ccgaaacgtg cgggggcaac cccgcacaca tcaataatcc ctcccttccc
    11761 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    11821 catcgaattt tcccgcgtca ccgaatcagc ggcgctggct ggctacaaat ggttaggacg
    11881 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    11941 ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    12001 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    12061 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    12121 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg
    12181 gccgggagcc aaaggcacca ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt
    12241 agcggcggcg ctcggcaaac cgttgagcga actgacggta acgattctgg ctaaaccacg
    12301 ccacgatgcc gttatcgctg aaatgcagca actcggcgta cgcgtatttg ctattccgga
    12361 tggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    12421 cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg gtgatccgcg cattagatgg
    12481 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaacg aagagaatcg
    12541 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    12601 attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    12661 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    12721 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tccatccact atctggatcg
    12781 caaagacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    12841 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    12901 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    12961 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    13021 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcctactcct atgttaactc
    13081 gcccgataat cccgatctgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    13141 acgattggcg gcactgaaac caggcgatga agtgcaggtg gttagcgaag cagctggctt
    13201 ctttgttctg gatgaagtac cggactgcga aacgctatgg atgctggcaa ccggtacagc
    13261 gattggccct tatttatcga ttctgcaact aggcaaagat ttagatcgct tcaaaaatct
    13321 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    13381 actggaaaaa cgctacgaag ggaaactgcg cattcagacg gtggtcagtc gggaaacggc
    13441 agcggggtcg ctcaccgggc ggataccggc gttaattgaa agtggggaac tggaaagcgc
    13501 gattggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    13561 ggtgcgcgat acacaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    13621 ccgaccgggc catatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    13681 acgatgagca aatccgcact catcttattg atcatcccgc gatatgccgg catcaaacgg
    13741 ttgataaatg agtgatgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    13801 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    13861 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    13921 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    13981 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gagctttatt caccagtaag
    14041 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    14101 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    14161 cctggcatga gaaagtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    14221 tgtaggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    14281 tggtcataaa agactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    14341 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    14401 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    14461 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    14521 tgatattgct taaggtgaag agttcagaag gattttccgg gtattgtcat ttgcatcgtg
    14581 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    14641 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    14701 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    14761 cgccccaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagcagcatc
    14821 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    14881 acagttactg ccgctgttgg ccaaagcaag cacacaaggc aaaacgccgc ggtctgaata
    14941 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    15001 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    15061 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    15121 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    15181 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    15241 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    15301 tccatcagcc agtttcgaga agactttaac agccagaatc ctagcctgcc actgaacgaa
    15361 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    15421 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    15481 caaatgacct ggctacccat ccaggggcca gagcaaaaag cagcgaaagc gaaagctcag
    15541 gaatacatgg cagcagtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    15601 aaaaaactgc agtcgctgct aacggcgggg aaaaacaaac gttattacac cgagacagaa
    15661 ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa
    15721 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    15781 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    15841 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    15901 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    15961 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    16021 tggcgaagcg cgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    16081 acctgtccgg cgcattcacc ggtgaaactt ctgccgctat gctgaaagac atcggcgctc
    16141 agtacatcat catcggccac tctgaacgtc gtacttacca caaagagtct gacgaactga
    16201 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    16261 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgacg
    16321 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    16381 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    16441 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    16501 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gatatcgacg
    16561 gcgcgctggt tggcggtgct tctctgaaag ctgacgcttt cgcagtgatc gttaaagctg
    16621 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    16681 ttccttaact cctcgcctta acgcaaaatc tcacactcat gatcctgaat ttcctcggct
    16741 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    16801 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    16861 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    16921 aggtattcat gcccgcgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    16981 agattgttat ccagttgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    17041 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    17101 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    17161 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    17221 aacagatatt gcagtggacc attaagatct tttaaaacca cgtatccggc attgggtttg
    17281 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    17341 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    17401 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    17461 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    17521 cgcgagaaac caccgggttt gataattatc ctggggaaat cagcgtttgc tgatctgatc
    17581 gaacataccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    17641 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ccacctcagc
    17701 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    17761 tttcaggtag gcttccgcca cctctttggt gccttttttc tcgaccactt tatcgaccac
    17821 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    17881 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcgatcagta catcgccaat
    17941 cccgcgctcg acaaaggtgt tagtggagcc gcgcgcgcca gaatccagaa cttcgacgtt
    18001 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    18061 cgcgtagccc caggcagcca ggtagttcca gcgtgcgcca ccagagcttt tcggattagg
    18121 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    18181 gcccttacgc accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    18241 tttgatccac tctttatcaa tccgcccgcg ttccgcaatt gcgtccacgt cataggccag
    18301 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttacctga
    18361 accaccgtgt gactgacgaa tcaccacgtt atcaccagtt tgctgtttcc agtgggcgct
    18421 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    18481 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    18541 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    18601 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgaaggat
    18661 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tccgctttgt ccatgccgga
    18721 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18781 aatgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggc
    18841 taaatgaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18901 cgcctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccaact
    18961 gttcgttctg gatacctacg caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19021 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19081 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19141 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19201 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19261 caacgaattc acagccaccg gcaatggccg cagccagggt cagatcgcca caataacggc
    19321 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19381 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19441 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19501 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgata ccacgttttt
    19561 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aaacgcgcag
    19621 aaccgaggaa cgtaccgcca cggttgatca tgtcagacac gctgtaacgg tctagctgta
    19681 ccatacggtc ttcatacaga cccagatagc cgtcataaat acccattact tccagacctt
    19741 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19801 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19861 gaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19921 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    19981 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20041 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20101 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20161 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20221 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20281 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20341 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20401 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20461 gcatatcagc ccgcaccgcc tggctttgcg tccgccgcac cacccaacgc tgaaacgaga
    20521 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20581 tcatcggtgt tggagatatc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20641 cggagataaa catactttgc gccagcgccg cgagggactc agctttaccg tgaccaaacg
    20701 agtgattatc gtcggcaggt tgcagggaat atcgcaccac caataaattc gtcaacgacg
    20761 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20821 accatgcaaa aattttaatc agcaatagca gcgaagccat cgccgtcgca gcaatcgccg
    20881 cccgactgac cagccgtcca taagattgat tcataaatac tcccgctatc aactgacgct
    20941 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21001 aaacccccac atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt
    21061 tactactggg aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat
    21121 tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc
    21181 tgctccggcg ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga
    21241 gcaatttgct cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt
    21301 tctgcggtga caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg
    21361 tgccgggcct gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg
    21421 ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta
    21481 tcgcctgaac cgacttcagc agcgtggctt aatgaactga ctgccagcgt tgaggccatg
    21541 acggcagcgg taactatgcg catcatttgc tcccaaaatc tttctgtcgc gattcaacga
    21601 tagagagttt acgattcagg ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc
    21661 atggattagc gacgtctgat gacgtaattt ctgcctcgga ggtatttaaa caatgaataa
    21721 aatcctgtta gttgatgatg accgagagct gacttcccta ttaaaggagc tgctcgagat
    21781 ggaaggcttc aacgtgattg ttgcccacga tggggaacag gcgcttgatc ttctggacga
    21841 cagcattgat ttacttttgc ttgacgtaat gatgccgaag aaaaatggta tcgacacatt
    21901 aaaagcactt cgccagacac accagacgcc tgtcattatg ttgacggcgc gcggcagtga
    21961 acttgatcgc gttctcggcc ttgagctggg cgcagatgac tatctcccga aaccgtttaa
    22021 tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc cgttcgcact ggagcgagca
    22081 acagcaaaac aacgacaacg gttcaccgac actggaagtt gatgccttag tgctgaatcc
    22141 aggccgtcag gaagccagct tcgacgggca aacgctggag ttaaccggca ctgagtttac
    22201 cctgctctat ttgctggcac agcatctggg tcaggtggtt tcccgtgaac atttaagcca
    22261 ggaagtgctg ggcaaacgcc tgacgccttt cgaccgcgct atcgatatgc acatttccaa
    22321 cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg
    22381 tcgcggctat ctgatggttt ctgcttcatg ataggcagct taaccgcgcg catcttcgcc
    22441 atcttctggc tgacgctggc gctggtgttg atgttggttt tgatgttacc caagctcgat
    22501 tcacgccaga tgaccgagct tctggatagc gaacagcgtc aggggctgat gattgagcag
    22561 catgttgaag cggaactggc gaacgatccg cccaacgatt taatgtggtg gcgacgtctg
    22621 ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa
    22681 ggccgcgtga tcggcgctga acgcagcgaa atgcagatca ttcgtaactt tattggtcag
    22741 gccgataacg ccgatcatcc gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg
    22801 ttctccgtgc gtgatggcga agataattac caactttatc tgattcgtcc ggccagcagt
    22861 tctcaatccg atttcattaa cttactgttt gaccgcccgc tattactgct gattgtcacc
    22921 atgttggtca gtacgccgct gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt
    22981 aagctgaaaa acgctgccga tgaagttgcc cagggaaact tacgccagca cccggaactt
    23041 gaagcggggc cacaggaatt ccttgccgct ggtgccagtt ttaaccagat ggtcaccgcg
    23101 ctggagcgca tgatgacctc tcagcagcgt ctgctttctg atatctctca cgagctgcgc
    23161 accccgctga cgcgtctgca actgggtacg gcgttactgc gccgtcgtag tggtgaaagc
    23221 aaggaactgg agcgtattga aaccgaagcg caacgtctgg acagcatgat caacgatctg
    23281 ttggtgatgt cccgtaatca gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac
    23341 cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag ccgagcagat gggcaagtcg
    23401 ttgacggtta acttcccgcc tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa
    23461 agtgcgctgg aaaatattgt tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg
    23521 ggctttgcgg tagataaaga cggtatcacc attacggtgg acgacgatgg tcctggcgtt
    23581 agcccggaag atcgcgaaca gattttccgt ccgttctatc ggaccgatga agcacgcgat
    23641 cgtgaatctg gcggtacagg attggggctg gcgattgttg aaaccgccat tcagcagcat
    23701 cgtggctggg tgaaggcaga agacagcccg ctgggcggtt tacggctggt gatttggttg
    23761 ccgctgtata agcggagtta aactccgcat ttgtaggcag gataaggcgt ttacgccgca
    23821 tccggcattt gagcaggatg cctgatgcga cgctgatagc gtcttatcag gcctacactc
    23881 cgggggttat tttccccaca accggcggga aaaatcttcg atcttgccgc tcagtcggcg
    23941 cttttgcatc gtccgcgtcc agcttttcga taacccggcg gcggagagta aacggtgata
    24001 ttgatcgtca tcaaacggca tatgccatgc gatggcggca gcctcctgca cggtcacatc
    24061 gctgacacgg gaaaccaact ccagcggcgc gtccgcagac actttccctg gcgctatcac
    24121 gctatacaac caccccacct tacctgtgtt ttgcatcaac tgcgctatat cgctgatatc
    24181 aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg ctgacctgaa ttaatgcctc
    24241 tccccagcgg aaaatatcgc ccatataaac attactttcc gtcaggccgt cggttgagag
    24301 gttttcacca aacgcaggcg caacaaacaa ctccgcctgt tccggaaatt cccgcgccca
    24361 gtagagataa tgctcacgag gataatgaca cagcgctctg tctggcccgc cgtgaacctt
    24421 tttctccgcc tgctcgtcac cttccagccc cagctccgtc agcatcaact caccatcaac
    24481 ctggattttg gcaattgcgc tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta
    24541 tacatcaacc ggatatcgca tctgttccct ctccacgaga aatttcccca ttagaccata
    24601 tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc caggcaggaa ctcaatagca
    24661 aaccggacga acgttagttc atccggctgt ggggattaag gaagcaggtt tatttcaccg
    24721 tacctaaaat ttcgattttc gctgctctgg ctttgacttt acgtgaccag atagaggtaa
    24781 gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc taccagcgaa gttgctgccg
    24841 gagccatcgg tttaaacgca ggcaccattt ctgcaatcag cacaggtgtc gctaccgcgg
    24901 cccctgcgga actggaagcg gcaattccgg cagtgccatc gccaccgcca atcaatttat
    24961 cggcgataat caacggaata ccggtcacga taattactgc cacacccaac aggatcccca
    25021 gcaaaccagt ctgggcaatc acagtcaaat caatggtatt gcccagcgcg aaggcaaaga
    25081 atggaatcag cgtttgcacc gctttgctga aaaattctcg taattcaggg tcaaggttcc
    25141 ccagggcaaa gcccaccagg aacggtaata ctgcgccgac gaaaacatgc ggttcaaacg
    25201 aggcaatccc tgcagtgccc agaataatca tcgtcatgag cggcccggac tccaacgaca
    25261 tcaacacaaa tgccccagct tcttcttttg tgccgtactg ctgcatgatg gaagcgtaaa
    25321 gtccgccatt ggtcatatcc atcgccgcca ccagcgccag cgttgaaagt ccggcaaaga
    25381 atccaacttc aacaccatgt tccggaataa tgcgcgaggc aattgccgca accacccacg
    25441 cgacggcaat ttttgttacc accagcgtac cggatttacg caataccgtt cccgtcgcgc
    25501 ttaattttat tgacgccccc atgcaaaaaa accacaccgc cagaatgggc accgtaccgg
    25561 taatcatccc gttggtgaat gatccaaaat atttccccgc ccccggcgag aaggtgtggc
    25621 acagtgcgcc aaggaatagc gggacgagca tcatcccccc cgggattttc tcaatcgagc
    25681 gttttatctg catttccatc acctgtcggc atatttttaa gggctgatgc gtcattcctc
    25741 aatggagcat cagtgttgat ggaaacgaag atagacattc atttaatcaa taaaagtgat
    25801 caaaaccaca tcaattgaaa cactgtttta tttttatcgg atcattgttt ttattttgaa
    25861 attgatcaca aaaaaaccac cgccgttggc gatggttcat tttttaagct gatatgcggc
    25921 ctatacgcct cattgcagca ggcggcaaat gattattttt tcgccgcgaa acgcgccgca
    25981 gcttcgtccc agttcaccac gttccagaac tctttgatgt agtccgggcg gcggttctgg
    26041 aatttcaggt agtaagcatg ttcccacaca tccaggccca gaatcgggaa gccggaagcg
    26101 ccagaaatag cttcacccat cagcggagaa tcctggttag cagtagaaac caccgccagt
    26161 ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa agcgggaagc tgccgctttt
    26221 tcaaattctg ctttgaagtt atcaacggag ccgaagtcac gttcgatagc cgctttcagg
    26281 tcaccctgca gggtggtgcc ttttttcaga cctttccaga acaggctgtg gttagcgtga
    26341 ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg gcagctggtc cagtttggtg
    26401 atcagctctt caaccggcag gttggcaaat tctggcaggc tttccagcgc cgcgttggcg
    26461 ttgtttacgt aggtctgatg gtgtttggtg tggtggattt ccatggtctg cttatcgaag
    26521 tgcggttcca gggcatcgta agcatacggc agggatggca gggtatagct catattcatc
    26581 tccagtattg tcgggcggcc gattgttaat gccgcgtaag cagttggttc attatagtta
    26641 attaaatgat attgaaaatg attatcaatg ccgtactttt cgtaagggta tggttttgca
    26701 ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg
    26761 acaacggcgg caacaggcga aaggttaatc gacagcacga tttttacact catctcgtcg
    26821 gagatgtgaa gcgacgaaaa atgatgagga taagaagatg agtaacgcga ttacgatggg
    26881 gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc cgttcaaaaa
    26941 agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt gggattgttt cgtggattat
    27001 tctgccgtgg gccatcagcg ccctgttact accggatttc tgggcgtatt acagctcgtt
    27061 tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga tcggtaatat
    27121 caactatggc ctgaccatgc gttatctcgg cctgtcgatg ggaattggca tcgccattgg
    27181 cattacgttg attgtcggta cgctgatgac gccaattatc aacggcaatt tcgatgtgct
    27241 gattaacacc gaaggcggac gcatgacgtt gctcggcgtt ctggtggcgc tgattggcgt
    27301 agggattgtg actcgcgccg ggcagttgaa agagcgcaaa atgggcatta aagccgaaga
    27361 gttcaacctg aaaaaagggc tggtgctggc ggtgatgtgc ggcattttct ctgccggtat
    27421 gtcttttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcac tgggcgtcga
    27481 tccactgtat gtcgctctgc caagctatgt catcatcatg ggcggcggcg cgatcatcaa
    27541 cctcggtttc tgcttcattc gtctggcaaa agtgaaggat ttgtcgctaa aagccgactt
    27601 ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcagcactgg gcgggttgat
    27661 gtggtatctg caattctttt tctatgcctg gggccacgcc cgcattccgg cgcagtatga
    27721 ctacatcagt tggatgctgc atatgagttt ctatgtattg tgcggcggta tcgtcgggct
    27781 ggtgctgaaa gagtggaaca atgcaggtcg ccgtccggta acggtgttga gcctcggttg
    27841 tgtggtgatt attgtcgccg ccaacatcgt cggcatcggc atagcgaatt aatctttctg
    27901 cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga
    27961 aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct
    28021 atgctggaga agatattgcg catgacacac tctgacctgc cgcagatatt gattgatggt
    28081 cattccagtc tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc
    28141 acaaaattta tccagcgcaa agggactttt caggctagcc gccagccggg taatcagctt
    28201 atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt
    28261 cagcaacatc accaactgcc cgaacagcaa ctcagccatt tcgttagcaa acgacacatg
    28321 ctgactactt tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca tccccacgct
    28381 acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg ctccctgcca
    28441 gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa ccagatcgtt
    28501 aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat
    28561 gcgataaggg cgatcgttga gtacatgcag accattaccg cgccagacaa tcaccagctc
    28621 acaaaaatca tgtgtatgtt cggcaaaaac atcttgcgga taacggtcag ccacagcgac
    28681 tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt
    28741 ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag
    28801 aagattcgcg ttattgcaga aaaccatccc gtccctgacg aatatcacgc ggtgaccagt
    28861 taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatcca cagcgatagg
    28921 cgatatcagt aacgctggcc tcgctgtggc gtagtagatg tcgggctttc atcagtcgca
    28981 ggcggttcag gtaacgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg
    29041 tacgcagcga aagagaaaat tgctccgcca cggcatccca attcacctca tcggcaaaat
    29101 ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttctcct
    29161 gcaaactgct tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg
    29221 tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct
    29281 gctgcaatac gctgtggtta acgcgccagt gggaaggata ctgcccatcc agctcttgtg
    29341 gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat
    29401 tagtcagaca cagattatcg gtatgttcat acagatgccg atcatggtcg cgcacaaaac
    29461 agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat
    29521 gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga
    29581 gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata
    29641 cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt
    29701 cttaccttaa attttcgacg gaaaaccacg taaaaaacgt cgatttttca agatacagcg
    29761 tgaattttca ggaaatgcgg tgagcatcac atcaccacaa ttcagcaaat tgtgaacatc
    29821 atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc
    29881 gcgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca cattatgacc
    29941 tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg ggcgcgtgat gctggcgcgt
    30001 tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc atcgttttaa caatgggctg
    30061 catagtcaga acggctatgt cacctgggat gtggatagcc tggaaagtgc cattcgcctt
    30121 ggattaaaca tggtgtgtga ggaagggatt cgtatcgata gcattgggat tgatacctgg
    30181 ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc cgttgcttat
    30241 cgcgatagcc gcaccaatgg cctaatggcg caggcacagc aacaactcgg caaacgcgat
    30301 atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca gttgcgtgcg
    30361 ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct gatgccggat
    30421 tacttcagtt atcgcctgac cggcaagatg aactgggagt acaccaatgc caccaccaca
    30481 caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg gagcggggcc
    30541 aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca ctggatttgc
    30601 ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt
    30661 atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct cttctggcac ctggtcattg
    30721 atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc caacatcacc
    30781 aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg cttatggctg
    30841 cttcagcgag tgcttcagga gcggcaaatc aacgatctcc cggcgcttat cgccgcgaca
    30901 caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt tattaatcct
    30961 gacgagatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc gatcccggga
    31021 agtgatgctg aactggcgcg ctgtattttc gacagtctgg cgctgctgta tgccgatgtg
    31081 ttgcatgagc tggcgcagct gcgtggtgaa gatttctcgc aactgcatat tgtcggcggt
    31141 ggctgccaga acgcgctgct caaccagtta tgtgccgatg cctgcggtat tcgggtgatc
    31201 gccgggccag ttgaagcctc gacgctcggc aatatcggca tccagttaat gacgctggat
    31261 gaactcaaca atgtggatga tttccgtcag gtcgtcagca ccaccgcgaa tctgaccacc
    31321 tttatcccta atcctgacag tgaaattgcc cactatttgg cgcagattca ctctacacga
    31381 cagacaaagg agctttgcgc atgaccactc aactggaaca ggcctgggag ctggcgaaac
    31441 agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct gcgccaactt gatcgtttac
    31501 ccgtttcaat gcactgctgg cagggcgatg atgtttccgg ttttgaaaac ccggaaggtt
    31561 cgctgaccgg tgggattcaa gctactggta attatccggg caaagcgcgt aatgccagtg
    31621 agctacgtac cgatctggaa caggctatgc ggctgattcc ggggccgaaa cggcttaatt
    31681 tacatgccat ctatctggaa tcagacacgc cagtctcgcg cgaccagatc aaacctgagc
    31741 acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct cggtctggat tttaacccct
    31801 cctgtttttc gcatccgcta agcgccgatg gctttacgct ttcccatgcc gacgacagca
    31861 ttcgccagtt ctggattgat cactgcaaag ccagccgtcg cgtttcggcc tattttggcg
    31921 agcaactcgg cacaccatcg gtgatgaaca tctggatccc ggatggtatg aaagatatca
    31981 ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc actggatgag gtgatcagcg
    32041 agaagctaaa ccctgcgcac catatcgacg ccgttgagag caaattgttt ggcattggcg
    32101 cggagagcta cacggttggc tccaatgagt tttacctggg gtatgccacc agccgccaga
    32161 cggcgctgtg cctggacgcc gggcacttcc acccgactga agtgatttcc gacaagattt
    32221 ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt cagtcgtccg gttcgctggg
    32281 acagcgatca cgtagtgctg ctggatgatg aaacccaggc aattgccagt gagattgtgc
    32341 gtcacgatct gtttgaccgg gtgcatatcg gccttgactt cttcgatgcc tctatcaacc
    32401 gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa agccctgctg cgtgcgttgc
    32461 tggaacctac cgccgagctg cgcaagttgg aagcggcggg cgattacact gcgcgtctgg
    32521 cactgctgga agagcagaaa tcgttgccgt ggcaggcggt ctgggaaatg tattgccaac
    32581 gtcacgatac cccaacgggt agcgaatggc tggagagcgt gcgggcttat gagaaagcga
    32641 ttttgagcca gcgtgggtaa acactgccgg atgcggcacg agcgccttat ccggcctacg
    32701 ggtcgaatgt ccatgcgggc tgtcaccctc accccggccc tctccctgaa agggcgaggg
    32761 agaaaaacat agcctcgtgc ggcaacggag ctgaacactg ctccggacag tcccctcgcc
    32821 cctacgggga gagggttagg gtgaagggaa caagcagcac catgtctaaa caaggcaacc
    32881 taatttacag gaacacagaa catgcaaaac attacccagt cctggtttgt ccagggaatg
    32941 atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg agcgcaacgg cggtaacctg
    33001 acgctgcgcc tggatgacgc cgatatcgcg ccttataaag acaacttcca tgcacaaccg
    33061 cgttatatcc cgctcagcca acccatgcct ttactggcaa atacaccgtt tattgtcacc
    33121 ggctccggca aattcttccg taacgtccag cttgatcctg cagctaactt aggcgtcgta
    33181 aaagtcgaca gcgacggcgc gggctaccat attctctggg ggttaaccaa cgaagccgtt
    33241 ccaacttctg aacttccggc tcactttctc tcccactgcg agcgcattaa agccaccaac
    33301 ggtaaagatc gggtgatcat gcactgccac gccaccaacc tgatcgccct cacctatgta
    33361 ctggaaaacg acaccgcggt ctttactcgc caactgtggg aaggcagcac cgagtgtctg
    33421 gtggtattcc cggatggcgt tggcattctg ccgtggatgg tgcccggcac ggacgaaatc
    33481 ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc
    33541 gtcttcggca gcgggtcgac gttggatgaa acattcggtt taatcgacac cgcagaaaaa
    33601 tcagcacaag tattagtgaa ggtttattcg atgggcggca tgaaacagac catcagccgt
    33661 gaagagttga tagcgctcgg caagcgcttc ggcgttacac cactagccag tgcgctggcg
    33721 ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc aggttaaccc cgacgctcac
    33781 gaagagtatc agcgtcggca taaccccatc tggccggaac tggaagcagt gttgaaatct
    33841 cacggtgcgc ataactacgc catttacctc gacaaagcgc gtaatctgct gtttgccacg
    33901 gtagagattg aatctgaaga gcgctggaat gcggttgcca gtactgatgt ttgccaacgt
    33961 tggtggaaat atatgaccga cgtcatgccc gcgaacccgg ataacagccc ggtgagtagc
    34021 gaactgcaag aagtgtttta cctgccgtaa ttgcctaatg tgccggagac gcctccggca
    34081 caatattttt aatttcgatt tgaaaaataa acatattaca ccttcagtta
//
LOCUS       DAZFVO020000038        33433 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFVO020000038 DAZFVO020000000
VERSION     DAZFVO020000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.038"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(25..603)
                     /gene="mocA"
                     /locus_tag="ACHKNB_RS08505"
                     /old_locus_tag="pgaptmp_001701"
     CDS             complement(25..603)
                     /gene="mocA"
                     /locus_tag="ACHKNB_RS08505"
                     /old_locus_tag="pgaptmp_001701"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HGU4195234.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            706..1476
                     /gene="yqeC"
                     /locus_tag="ACHKNB_RS08510"
                     /old_locus_tag="pgaptmp_001702"
     CDS             706..1476
                     /gene="yqeC"
                     /locus_tag="ACHKNB_RS08510"
                     /old_locus_tag="pgaptmp_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HGU4195235.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1524..3149
                     /gene="yqeB"
                     /locus_tag="ACHKNB_RS08515"
                     /old_locus_tag="pgaptmp_001703"
     CDS             1524..3149
                     /gene="yqeB"
                     /locus_tag="ACHKNB_RS08515"
                     /old_locus_tag="pgaptmp_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HGU4195236.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTMLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3190..4122)
                     /gene="arcC"
                     /locus_tag="ACHKNB_RS08520"
                     /old_locus_tag="pgaptmp_001704"
     CDS             complement(3190..4122)
                     /gene="arcC"
                     /locus_tag="ACHKNB_RS08520"
                     /old_locus_tag="pgaptmp_001704"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4195237.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4170..5555)
                     /gene="hyuA"
                     /locus_tag="ACHKNB_RS08525"
                     /old_locus_tag="pgaptmp_001705"
     CDS             complement(4170..5555)
                     /gene="hyuA"
                     /locus_tag="ACHKNB_RS08525"
                     /old_locus_tag="pgaptmp_001705"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HGU4195238.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5608..6819)
                     /gene="ygeY"
                     /locus_tag="ACHKNB_RS08530"
                     /old_locus_tag="pgaptmp_001706"
     CDS             complement(5608..6819)
                     /gene="ygeY"
                     /locus_tag="ACHKNB_RS08530"
                     /old_locus_tag="pgaptmp_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HGU4195239.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6877..8073)
                     /gene="dpaL"
                     /locus_tag="ACHKNB_RS08535"
                     /old_locus_tag="pgaptmp_001707"
     CDS             complement(6877..8073)
                     /gene="dpaL"
                     /locus_tag="ACHKNB_RS08535"
                     /old_locus_tag="pgaptmp_001707"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HGU4195240.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8131..9318)
                     /gene="ygeW"
                     /locus_tag="ACHKNB_RS08540"
                     /old_locus_tag="pgaptmp_001708"
     CDS             complement(8131..9318)
                     /gene="ygeW"
                     /locus_tag="ACHKNB_RS08540"
                     /old_locus_tag="pgaptmp_001708"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HGU4195241.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9797..11575
                     /gene="ygeV"
                     /locus_tag="ACHKNB_RS08545"
                     /old_locus_tag="pgaptmp_001709"
     CDS             9797..11575
                     /gene="ygeV"
                     /locus_tag="ACHKNB_RS08545"
                     /old_locus_tag="pgaptmp_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HGU4195242.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGLGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(11615..12094)
                     /gene="xdhC"
                     /locus_tag="ACHKNB_RS08550"
                     /old_locus_tag="pgaptmp_001710"
     CDS             complement(11615..12094)
                     /gene="xdhC"
                     /locus_tag="ACHKNB_RS08550"
                     /old_locus_tag="pgaptmp_001710"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HGU4195243.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12091..12969)
                     /gene="xdhB"
                     /locus_tag="ACHKNB_RS08555"
                     /old_locus_tag="pgaptmp_001711"
     CDS             complement(12091..12969)
                     /gene="xdhB"
                     /locus_tag="ACHKNB_RS08555"
                     /old_locus_tag="pgaptmp_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HGU4195244.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12980..15277)
                     /gene="xdhA"
                     /locus_tag="ACHKNB_RS08560"
                     /old_locus_tag="pgaptmp_001712"
     CDS             complement(12980..15277)
                     /gene="xdhA"
                     /locus_tag="ACHKNB_RS08560"
                     /old_locus_tag="pgaptmp_001712"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HGU4195245.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15692..16447
                     /gene="actS"
                     /locus_tag="ACHKNB_RS08565"
                     /old_locus_tag="pgaptmp_001713"
     CDS             15692..16447
                     /gene="actS"
                     /locus_tag="ACHKNB_RS08565"
                     /old_locus_tag="pgaptmp_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HGU4195246.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16526..16599
                     /locus_tag="ACHKNB_RS08570"
                     /old_locus_tag="pgaptmp_001714"
     tRNA            16526..16599
                     /locus_tag="ACHKNB_RS08570"
                     /old_locus_tag="pgaptmp_001714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16558..16560,aa:Gly,seq:ccc)
     gene            16764..17978
                     /locus_tag="ACHKNB_RS08575"
                     /old_locus_tag="pgaptmp_001715"
     CDS             16764..17978
                     /locus_tag="ACHKNB_RS08575"
                     /old_locus_tag="pgaptmp_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4195247.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSQSYAAEMIDTFEKDVFPYIGSKPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRQEMLQWYADYMDSLEQG
                     GNVVHGKFGKCG"
     gene            18077..18781
                     /locus_tag="ACHKNB_RS08580"
                     /old_locus_tag="pgaptmp_001716"
     CDS             18077..18781
                     /locus_tag="ACHKNB_RS08580"
                     /old_locus_tag="pgaptmp_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195248.1"
                     /translation="MPIVENIPDLSHWRTVQEFSITQAALLLAGIDPYDYPTLEHARD
                     YKHERWKMAWGLANGMVTAIRRGVLTPVWIEYYPLWEVQRASGTPRTEIPDRLKKISM
                     DETIITRHSLLEWVRNERVDYVRPPRSISDKEIKPSNQSVIIDAESRVINSEETVLLT
                     YGHKSEGLEYVEDAIRELWATYDPDNQQTAPTRNDVVEYLKGRGAGQNMAEAVNLILR
                     PTGLQQVGRRKRKTNS"
     gene            19029..19226
                     /locus_tag="ACHKNB_RS08585"
                     /old_locus_tag="pgaptmp_001717"
     CDS             19029..19226
                     /locus_tag="ACHKNB_RS08585"
                     /old_locus_tag="pgaptmp_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4195249.1"
                     /translation="MSNNLLRLSDVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSVAF
                     IESEVDEWINQRIDASRNNAA"
     gene            19291..20010
                     /locus_tag="ACHKNB_RS08590"
                     /old_locus_tag="pgaptmp_001718"
     CDS             19291..20010
                     /locus_tag="ACHKNB_RS08590"
                     /old_locus_tag="pgaptmp_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4195250.1"
                     /translation="MKRPNMPVQGQGFIQPEISQNDILIRHKAESRIDSRVISERAGI
                     QHESIIATIKSHQSRLRELGSLPRQSVKELTYLKSGKSGRKRGRPEISYLLNELQLDY
                     LLRVIRGRDAERINQFKLDVTKAFAKHRAAEPVRREYLPGYHESRDGLKALGAERHHY
                     INLARTENRFAGLAVGERQSAGEQQLGLLIVMQKIEQAAFNEAIQNGMTPTEAVREVA
                     RRMETFASLMTSAPSLGVNHA"
     gene            20003..20176
                     /locus_tag="ACHKNB_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /pseudo
     CDS             20003..20176
                     /locus_tag="ACHKNB_RS08595"
                     /old_locus_tag="pgaptmp_001719"
                     /inference="COORDINATES: protein motif:HMM:NF019945.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(20893..21072)
                     /locus_tag="ACHKNB_RS08600"
                     /old_locus_tag="pgaptmp_001720"
     CDS             complement(20893..21072)
                     /locus_tag="ACHKNB_RS08600"
                     /old_locus_tag="pgaptmp_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4195251.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            21338..21469
                     /locus_tag="ACHKNB_RS08605"
                     /old_locus_tag="pgaptmp_001721"
     CDS             21338..21469
                     /locus_tag="ACHKNB_RS08605"
                     /old_locus_tag="pgaptmp_001721"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4195252.1"
                     /translation="MQCLTGSIAIFTVTAATEREARARLPHAHLIFVARIRQEASHG"
     gene            21815..22000
                     /locus_tag="ACHKNB_RS08610"
                     /old_locus_tag="pgaptmp_001722"
     CDS             21815..22000
                     /locus_tag="ACHKNB_RS08610"
                     /old_locus_tag="pgaptmp_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195253.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            22004..22213
                     /locus_tag="ACHKNB_RS08615"
                     /old_locus_tag="pgaptmp_001723"
     CDS             22004..22213
                     /locus_tag="ACHKNB_RS08615"
                     /old_locus_tag="pgaptmp_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195254.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGEMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            22258..22449
                     /locus_tag="ACHKNB_RS08620"
                     /old_locus_tag="pgaptmp_001724"
     CDS             22258..22449
                     /locus_tag="ACHKNB_RS08620"
                     /old_locus_tag="pgaptmp_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195255.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            22626..24764
                     /locus_tag="ACHKNB_RS08625"
                     /old_locus_tag="pgaptmp_001725"
     CDS             22626..24764
                     /locus_tag="ACHKNB_RS08625"
                     /old_locus_tag="pgaptmp_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HGU4195256.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGKLA
                     GDKTPPVVLADEQLKDIGNLQIVPDGRKSARIYKAGHLDQVMVKGIGQKLAAAGVQDA
                     DYYPEGMHHNERQNWRNYLETERKNISDGLVIELPVKKKTSEQSEDELKPRVECRPDG
                     VYWVTPKVDKQSGEIIRPCEWIGDRMRTVGIGHDGCDGYLIIELEPEGMEKIIYEAMP
                     RNEIGLPSGWSRLRGRGVAVTTSQKLLNKLAEYLQREGERTQWEVTSTAGWHCGAYVM
                     PDGEIIGVPERPVAFCGGSAAIKGYVVRGTADEWRDNVASLMRGNHSMMLGVLVGLAA
                     PLNSLVGGGCFGVHLFAQSSAGKTTTVEAATSLYGDPEVLKLSWDATRYGLTVEAAAR
                     NDGFIPIDEIGQGGRINDIAQSAYSLFNGVGRIQGRKDGGNRAVIRWRIAALSTGEED
                     FETFLIKGGVTPKAGQLVRLLSVPFIDTTVFNGYDDGDQHARAIKRFASNYCGAAGRE
                     WIGWLSANKELAVSVVNEKENIWLSRLPENASPQVKRVASRFAMLDAAGELATGITGW
                     TTGESHAATQRAFDDWLQDFGLDNREKYQVISRARDFIQRHALSRFQPYTYGRQNGDM
                     DVNYGARITSLAGYLVRGRRDDGLPEYHIIPSVFDEEILCGINRNFGCQALKEAGILI
                     HAGDKNWTTKTIKVNGIQQRFIVLLDQSEE"
     gene            25230..25553
                     /locus_tag="ACHKNB_RS08630"
                     /old_locus_tag="pgaptmp_001726"
     CDS             25230..25553
                     /locus_tag="ACHKNB_RS08630"
                     /old_locus_tag="pgaptmp_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195257.1"
                     /translation="MNSDIKTLSISGALPGWWARFKDDDGTEWYSPIAAWALCEVAPC
                     NTGCAYQEILPVLPGEAGMEPHYSDCGACECLYLPDKKFVHCGESWVFAWYPVNDGSN
                     SGTLE"
     gene            25557..25799
                     /locus_tag="ACHKNB_RS08635"
                     /old_locus_tag="pgaptmp_001727"
     CDS             25557..25799
                     /locus_tag="ACHKNB_RS08635"
                     /old_locus_tag="pgaptmp_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195258.1"
                     /translation="MKVSHKKTDHRAIDITEHWLRVAIKIIDRNTGEGYAKEHPELIS
                     AFMTTAAANFATLTEREIAEAEQVATINVKTGEVES"
     gene            25796..26245
                     /locus_tag="ACHKNB_RS08640"
                     /old_locus_tag="pgaptmp_001728"
     CDS             25796..26245
                     /locus_tag="ACHKNB_RS08640"
                     /old_locus_tag="pgaptmp_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4195259.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMALARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            26262..26729
                     /locus_tag="ACHKNB_RS08645"
                     /old_locus_tag="pgaptmp_001729"
     CDS             26262..26729
                     /locus_tag="ACHKNB_RS08645"
                     /old_locus_tag="pgaptmp_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4195260.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKEQRKNHRRINRIAEYWPE
                     LFGTENIKPLKAGIIRDVLEDAKSRELPLATGVLRGALTSHLHTTRYLKAIIAGGPRY
                     DLNGQPCGEVTEKEKAAASEQLMWRLEQIKQQNIQWQDTGGGESDNRQRGTET"
     gene            26698..26991
                     /locus_tag="ACHKNB_RS08650"
                     /old_locus_tag="pgaptmp_001730"
     CDS             26698..26991
                     /locus_tag="ACHKNB_RS08650"
                     /old_locus_tag="pgaptmp_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4195261.1"
                     /translation="MITDSVAQKLEERGLWRRAATRCSDVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWKNYSFSDAGDDLALS"
     gene            complement(27442..27828)
                     /locus_tag="ACHKNB_RS08655"
                     /old_locus_tag="pgaptmp_001731"
     CDS             complement(27442..27828)
                     /locus_tag="ACHKNB_RS08655"
                     /old_locus_tag="pgaptmp_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195262.1"
                     /translation="MRLTQYKKEILSYFTPDNLSWVRGEIGNPPFDVSGVAYLLYGID
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERREGTGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVDGQ"
     gene            27913..28215
                     /locus_tag="ACHKNB_RS08660"
                     /old_locus_tag="pgaptmp_001732"
     CDS             27913..28215
                     /locus_tag="ACHKNB_RS08660"
                     /old_locus_tag="pgaptmp_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4195263.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKSDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            28217..28384
                     /locus_tag="ACHKNB_RS08665"
                     /old_locus_tag="pgaptmp_001733"
     CDS             28217..28384
                     /locus_tag="ACHKNB_RS08665"
                     /old_locus_tag="pgaptmp_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4195264.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            28909..29070
                     /locus_tag="ACHKNB_RS08670"
                     /old_locus_tag="pgaptmp_001734"
     CDS             28909..29070
                     /locus_tag="ACHKNB_RS08670"
                     /old_locus_tag="pgaptmp_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195265.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            29568..29960
                     /locus_tag="ACHKNB_RS08675"
                     /old_locus_tag="pgaptmp_001735"
     CDS             29568..29960
                     /locus_tag="ACHKNB_RS08675"
                     /old_locus_tag="pgaptmp_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4195266.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(30791..31165)
                     /locus_tag="ACHKNB_RS08680"
                     /old_locus_tag="pgaptmp_001736"
     CDS             complement(30791..31165)
                     /locus_tag="ACHKNB_RS08680"
                     /old_locus_tag="pgaptmp_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195267.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            31870..>33433
                     /locus_tag="ACHKNB_RS08685"
                     /old_locus_tag="pgaptmp_001737"
     CDS             31870..>33433
                     /locus_tag="ACHKNB_RS08685"
                     /old_locus_tag="pgaptmp_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195268.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STPDQIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAI
                     VYKESRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSVTLHE
                     APSPIIPLNWSKA"
BASE COUNT         8827 a         7760 c         8656 g         8190 t
ORIGIN      
        1 aacgcaatcg tttaactaca ttttctaaat tttagtatac cttttttgcg ctgtaataaa
       61 gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatctcga cggaataatg
      121 agctcccata aggagtgcct gacgcatatt ggtgacattg ggtcgttgga ttgcctgcat
      181 caagcatggt tttgacacta aaatcggatg gccggggatg ccattatgga gtggcagtat
      241 tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga gggttggcat
      301 gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg ggtactgcgg cctttactga
      361 tgttagtaaa ccctgcgcat aatctgggtt gtggataata gtgatattgc tctggttcgc
      421 ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc ggctacaaaa
      481 ctgcaacgca tttttgatac ttgtatcaag aattgtcccc tgttgccagg gtaacatcat
      541 tttccattgc cccattcttg atgataatcc ggcagcggta attatacagt cgatggctga
      601 catagtggtt ccattattta taggggctaa taacgcctga taattgcgtt acttcctgat
      661 ggattaagtt ggggataatt tatcccagag aggtcataaa gactcgtgaa aagtatagtt
      721 gacccatcgg cattagtcat tgatttaggt gcgcagaaac gtcccactgt gatttctgtt
      781 gttggtgctg gggggaaaac cagcttgctt ttttggctgg cagaactgct ccaggctagc
      841 ggcaggcgtg tgttaatcac gacgacgaca catatgttta tgccaacgtc tcactggccc
      901 gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt ctcttacatc ccccatttca
      961 ttttgttttc acagctggaa agcgaaccag ggaaaagtgc agggatttac gccagaagcg
     1021 attgatgcac tggtgcaacg accagagtgt gacgtaattc tcattgaagc agatggctcg
     1081 cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt gcatacccaa aagcagttgc
     1141 tgcgtgattg ctgtgatggg aggacatact ttgggcgcga aagtgagcac agaaaatgtc
     1201 catcgctggt cgcagtttgc tgatattacc gggttaacac ctgatgcaac cttgcaactg
     1261 agcgatctcg ttgcgctggt tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt
     1321 tgtcggcgag tctggttcat taaccgtttt tctcaatgtg agaatgcgat tgcacaaagc
     1381 gagctccttc aaccgctgca acaacacgac gtagaggcaa tctggctggg cgatatacaa
     1441 gaacatcctg caatcgcgcg cagatttgtg aattagcggc ccgggtattc cgggttactg
     1501 aaaggatatc cattgaggtt ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc
     1561 aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg aggctcgact ccccgccatt
     1621 ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg
     1681 ttgagcggaa ggtgattgaa gagtcacttc aggcattgca ggaacgtaag ccgcgattat
     1741 tccatggacg tatggctcgt aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa
     1801 tgtcagtatt tatcagcgtc catggtatgc gtccacgtct ggtgttgatt ggcgcggggc
     1861 atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg atttgatatt gccgttgccg
     1921 atatttatcg cgaaagcctc aatcctgaac tattccctcc atcaaccatg cttctccatg
     1981 ctgagtcgtt tggtgcagca gtggaagcac tggatattcg ccctgataat tttgtcctga
     2041 ttgccacgaa taatcaggat cgtgaagccc tcgacaaact cattgaacag cccattgcat
     2101 ggttggggtt gctggcaagt cgccgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga
     2161 aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc cgttggttac aacataggtg
     2221 cggaaacgcc gcaggagatc gccatcagcg tgttggcaga aatattacag gtgaaaaata
     2281 acgcgccggg tgggctgatg atgaaacctt ctcatccttc cggacaccag ctggtggtga
     2341 ttcgcggtgc gggagatatc gccagtggtg tggcgctacg tctgtatcat gcgggtttta
     2401 aagtgatcat gctggaagtg gaaaaaccga cagtgattcg ttgtacggtg gcgtttgccc
     2461 aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac tgctcgcctg gcaaccagct
     2521 ctgcggaagc gatgaaactt accgaacgcg gattcatccc tgtgatggta gatcccgcct
     2581 gttcattgct tgatgaactg aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga
     2641 atttgggaac gcgggcagat atggcaccag taacaatcgc gcttgggccg ggctttactg
     2701 cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga
     2761 tttactctgg ttgtgcgcag gagaataccg gtgttcctgg caatattatg gggcatacca
     2821 cccgacgggt gatccgtgct cctgctgcag gcattatgcg atccaacgtg aaattaggcg
     2881 atctggtgaa agagggcgat gtgattgcct ggattggtga gcatgaaatt aaagcaccgt
     2941 tgacggggat ggtgcgtggc ttgttgaacg acggcttggc agtggttggt ggttttaaaa
     3001 ttggtgatat cgatcctcgt ggtgaaaccg ctgatttctc cagcgtttct gataaagccc
     3061 gggcgattgg cggcggcgta cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag
     3121 cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc cggatggtat tacccacccg
     3181 gccttaaaat tactgacaaa tcaccgttcc ggttttgcct tcaatccctt ctttcgcttt
     3241 gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc
     3301 ttccacttta ggcagcatag aacctttcgc aaaatgacct tcttcgataa agcgttcagc
     3361 atcacttaat gacaggcgat cgagccactg ttcattctct ttaccgaagt taatggccac
     3421 tttttctacc gcagtcagaa tgatcagcat atcggcatca atcatttctg ctaaacgggc
     3481 gctggcccag tctttatcga taaccgcgct ggcaccacgc agatggttac cttcacgaat
     3541 aacaggaata ccaccaccgc caacggtgat caccacctgg cctgcatcta ccagagcttt
     3601 aaccgtttct ttttcaataa tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc
     3661 gcgaccagca tcttctttca gagtgtaacc ctgttttgtc agttgctctg cttcttgctc
     3721 ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac
     3781 ttcgacctga gtaaccagcg ttgctaccgg tttattaatg ccccgagaaa gcagttcttc
     3841 tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg cttaaggcaa cgcagacaga
     3901 catcggcagc attggcgagt gcgcttcagt ttttgcagca gcttcaaagg cctggttaat
     3961 catgcccacc tgcggcccgt taccatgagt gacgataact tcatgtcctt gggcaattaa
     4021 atcaacaatt gcctgagaag taatttttac cgctttcatt tgtccggcca ggtcatcgcc
     4081 cagcgcattc ccgcccaggg cgagaacaat tttcttactc atttttcctc actcatcatt
     4141 cttgattgtg tatagatccc tgacagtggt tagagcacgg gagggacaaa cggtttgcgt
     4201 cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca
     4261 ccacgggata aggttctgac aatcgcgccc tgacaggtaa aaccctccca tggcgagtag
     4321 tcggcgttgt cgtggagatg gcgatgctga atttgttggc tctgacgtgg gtcgataatc
     4381 accacgtcgc catcggaacc gggcgctaat aatccttttt gcggccacag gccaaacagc
     4441 ctggcgggca ttgcgctggt taattcaaca aagcgttccg gtgttatacg tcccgtcatc
     4501 acgccactgg aaaacagtaa ctgcatgcgg ttttccacac cgggtaagcc atttgggcag
     4561 cgactgaaat cgcctttaga aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg
     4621 gtcgccacca cgtcaatcgc accatcgctg atgccacacc acagtttgtc ctgctcgcgt
     4681 acgttacgca gcggtgggct aagaatgaac ttcatgccgt cttctgtatc gtaactgcgt
     4741 tcgtccaaca ggagatattg tgggcaggtt tcaacccaga ctggctggtg attcgcacgg
     4801 gcaagacgca gataatccag acctaagccg ttagacaggt gcacgatata gagcggggcg
     4861 ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg cttccgcttc gcattccaga
     4921 gggcgactca aggcatgata gcgcggcgcg gttaaccctg cggcgataaa ttccgcccgc
     4981 ttgctggcga tagccgcatc attttccggg tgcacggtgg tcagcgcgcc ggattcatgc
     5041 agacggcgta atgcctgcaa aacctcgtca tcgttgagtt tgtattgata ggttaaatag
     5101 agtttaaaac tgctcagtcc ttcctcgacc atcatcggga tttcgtcgag gattgcgtga
     5161 ttaatgtgct ggatcacacc gtgaaagctg taatcgatga ccgctttatg ggcggcataa
     5221 ccacgataaa cctccagttg atggcgtaac cgacagccgt ttgggccaaa tcccatatgg
     5281 tcaataatgg ttgttgtacc gccacacgca gctgcgcggg taccggtaaa aaaatcatca
     5341 caactgcgcg cgatgccgac atcaatattg aaatgcgtat ggacatccac gccgccaggg
     5401 aaaacgtaac agccagtggc atcaatttct tcatacggga gctgcggcga aatattgttg
     5461 cccaactggc gaacaatccc gctttcaatc agcaaatcct gtttggcttg tccatctgcg
     5521 ttaacgacag tgccgttttt gatcaatacg cgcatagcaa actccaaaaa ccctccggcg
     5581 aaccggaggg gaataagtga agagtaatta ctctgttgcc agccagctta acgggattgc
     5641 agcgtacatc gcggcacagg tcaccaggtg agatttccag gttttttcgt taggtgcatg
     5701 cgcttcaggt tctttacccg ggccaaagcc gatcaccgga atgccgtgac ggcccatgat
     5761 agatacgccg ttagttgaga aggtccactt atcaacaacc ggcgctttgc caaacagccc
     5821 ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct tcttccactt tccaggtcgg
     5881 gaagtagcat tcggttgggt aaaccaggcc agtccaggac ggacggtcgt agttgtacat
     5941 agaaacaacc gcgttagctt tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc
     6001 ttcccaggtt tcgccccagg tcagacggcg gtcaatagag acggcgcagc tatctgctac
     6061 agcgcaacgg cttggggatg tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag
     6121 gaattcgtca taacccagac gttgggagag ttcttgtaat tcgccaagaa tcggacccat
     6181 tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg tggcagctaa caccctgaac
     6241 atcaatacga atttccatac gaccgcgctg accacggtat acctggcagt cggttggttc
     6301 ggtactgacc acaaattccg ggcgaatgcc ggattgttca ataatgtact gccagcacag
     6361 accgtcgcag tcttcttcct gcacagtacc ggtaaccagc agggtatatt catcttccag
     6421 accgaggtct ttaatgattt taccggcata aaccatagat gccatgccgc cttcctggtc
     6481 ggaagtaccg cgaccaccga tcagctcatc agtttccatg ccttcgtacg gatcgaagtc
     6541 ccagtttttg atgttgccaa tgccgacggt atcgatatga gcgtccattg ccaccagacg
     6601 cgggccgtgg ccgatataac cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa
     6661 gccgactttt tccatctctt ctttaatacg atgtactacg cgtttttcgt cgcagctttc
     6721 actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc
     6781 tttttcaaga atcagtttga atggaatatt cttagccatt atttttctcc agatttttta
     6841 ctccccgttt tgcagggagt agttccaata gggtgattaa ggtgctacag cgtgtttgcc
     6901 ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg ccttcagtgc tgataaccag
     6961 cactacggca tctttgttca gcgccagttt ttccatcagg ctttgacgtt gcgggtgata
     7021 atgaaccgct gcgagaacgc ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg
     7081 gtcgttgccg tacggattac ccagcacgcg catacctaat gcggcaacgc tgtcctggca
     7141 ggagatgaat tgggtggcgc agttacgtag gatttcccag cccagcgggt taggttcgcc
     7201 acaggccagg cccgccatga tggtggccat atcaccgcca acgttgacga tgtcgccttt
     7261 gacgccggag cgataaatac agtcagcttt gtcaggttca acaataatgc tgtgcagatt
     7321 ttgcgggctg tagacgtcga ccagataacc cagcacacca ccggccattg ctccgacacc
     7381 ggcttgcagc agaacgtgcg tcggggttac gcccatttca cgcatttgct cgacggcttc
     7441 atctgccagg gttgcgtagc cttgcatgat ccaggttggg attttggtgt aaccttccca
     7501 tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac
     7561 ggtatcgtca tagttcatat ccgtgacgat gcactcggca ccgaggttca gaatggcgtc
     7621 aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc gcattctgtc cgagttgctg
     7681 tgctgcccac gccacaccgc gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt
     7741 ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt
     7801 ttcacacaat aattgagcga tggcgtacgc accgccaagc attttgaagg cgttcagacc
     7861 gaatcgtttt gattcgtctt tgacgagaat tttcttcaca ccaaaaaggt ttgcgagatc
     7921 gtccagcgca caaagcggtg ttgggcgata accagctatt ttctggtgga actggcgcgc
     7981 cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc
     8041 ggcgatatca atcttcaatg agaaaacgga catatcctat ccttttggcc tctggaaata
     8101 gagggccgac cagctgatcg gcccgagggg ttatttcacg cgttcttgcg cttctttcag
     8161 cagttgctcg agcagtgcac ctggtttggc gtatttacgg gacaggatca tcgcggcgat
     8221 gatataaggc ttccagctgg cttctttgta ggtagcgata cggtattttt cgaatacgcc
     8281 ttcagtcact tccccttctt tgcaggatac gccgctgata tcagctggca ggcagtgcat
     8341 gtacagggct tcgccatcac gggtcagttc catcatctct tcagtacaat gccagtcttt
     8401 gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct tttaagcctt cgtgatcgtt
     8461 agcgcgcagc aattcggtac gctgttccat cactttgtaa ggtgcccatg acttcggata
     8521 aacgatgtct gcatctttga atgcttcttc catgctggtg acctgacgga agctaccgcc
     8581 agatgcttta gcattgtttt tcgcgacttc tactacatcc gggatcaggt cgtagccttc
     8641 cggatgggcc agggtgacat ccataccgaa gcgagtcatc agaccgatga tgccttgtgg
     8701 tacagagagc ggtttgccat agcttggaga gtaggcccag gtcatggcga tttttttacc
     8761 tttcaggttt tccagtgaac caaagtgttc acgtaaccac gcgaggtcag ccattgactg
     8821 agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc ggacgctgtg gcagtacacc
     8881 ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc
     8941 cagatacata tcgtcgcgaa taccaatagc gtcggcgcag aaggagatca tattggcggt
     9001 ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc
     9061 aaggccgagc aggttaagcg cggaagcata agagaagcgg gtacgagtgg agttgtcgcg
     9121 gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt cagcacgcag
     9181 tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg tctgttccca
     9241 cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat ccttaatcag
     9301 ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa gtggtttatt
     9361 tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac taacaataag
     9421 tgtgccagaa ttatccacgg ggctgtaaaa gactaatttg acggataatg tgtggggttg
     9481 atcacaaatt gatgccagct tcgggttgtt tgtaaaatac gaaaacgatt ggcttttctt
     9541 atcgcgttat ttatttactg ctaagtatca gaaataagat aaagggacag gctatctctc
     9601 ttcagtttga taaactcacc gccgtaattc tcatattgat atagaaatgt gatatctctt
     9661 ccataatttc attagaaagt gataaaaatc ttatgttttt atcaaagcca atttcgcctg
     9721 agttaatatc atcatgatct gtgaaccatc aacgtcttca atgatcgact tttaagtaag
     9781 tctggaaggt aaagctatgg agcttgctac tacgcagtca gtattgatgc aaattcaacc
     9841 gacaattcag cgttttgcca gaatgcttgc cagcgttttg cagcttgagg ttgagatcgt
     9901 tgatgaaaac ttgtgtcgcg tcgccggaac tggcgcgtat gggaagtttc ttggccgcca
     9961 gttgagcggc aactcacgcc tgctccgcca cgtcctggaa acgaaaactg aaaaagttgt
    10021 gacacagtct cgcttcgatc ccctttgcga aggttgcgat agtaaagaaa attgccgcga
    10081 aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt tgtgttgggg tgataagttt
    10141 gattgccgtt acccacgagc aacaagagca tatcagtgat aatttacgcg aattttctga
    10201 ttatgttcgc catatatcca ccatttttgt ttcgaaactt ctggaggatc aggggctagg
    10261 agataacatc agtaaaatat tcgcgaccat gatcgataat atggatcagg gcgtattagt
    10321 tgttgatgat gaaagtcggg ttcagtttgt taatcagact gccttaaaaa cacttggtgt
    10381 tgtacaaaat aatattattg ggaaacctat ccgtttcaga ccattaacat ttgagagtaa
    10441 ttttactcat ggacatatgc agcatattgt ttcgtgggac gataaaagtg aattaatcat
    10501 tggtcaattg cataacattc agggccgaca attattttta atggcatttc accaatcgca
    10561 taccagtttt tctgtagcaa atgcacctga tgaaccacat attgaacaat tggttggcga
    10621 gtgccgtgtt atgcggcaat taaaacgact cattagccgt attgcaccca gcccatccag
    10681 cgttatggtg gttggtgaaa gcggcacggg taaagaagtc gtcgcccgag caatccataa
    10741 gttgagcgga agacggaata aaccctttat tgctatcaac tgtgccgcga ttccggagca
    10801 gcttctggaa agcgaactgt tcggttatgt taaaggcgca tttactggcg cttctgccaa
    10861 cggtaaaaca gggttgattc aggcggcgaa tacgggcacg ctgtttctcg atgaaatagg
    10921 tgatatgcca ttaatgttgc aggctaaatt actgcgcgct attgaggcgc gtgaaattct
    10981 gccgattggt gccagtagcc caatacaagt cgacattcgc atcatttctg caactaatca
    11041 gaatttggcc cagttcattg ccgaaggtaa attccgcgaa gatctcttct accgacttaa
    11101 tgttatcccg ataactctgc caccgctgcg tgaacgtcag gaagatattg aactattggt
    11161 gcattacttt ttacatctgc atacccgtcg tctgggatcg gtttatcctg gcattgctcc
    11221 cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga aacctgcgcg agttaagcaa
    11281 tttgatggaa tatctggtta acgtagttcc ttcaggtgaa gttatcgaca gcacgctatt
    11341 gccgccaaat ctgctgaata atggcacaac ggagcaaagt gatgtaacag aggtcagtga
    11401 ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg gaggagatgg aaaagcaaat
    11461 gatccgcgag gcgctttcac gtcataacag caagaagcaa gttgctgatg aactgggcat
    11521 cggcattgct acgctctatc gcaagattaa gaaatatgag ttgttaaaca cataacctca
    11581 gtgaatccgg cctgaattca ggccggatat ccttttactt cgttttctcg caatccagaa
    11641 ctgtatttac aatcatctga taccccgtgc agcgacaaag atttcccgcc agtccgcgac
    11701 gaatttccgt aatggttaat ggcttctcgc gtggtttcgc cagcattgcc gtggtagcca
    11761 taatcaggcc aggcgtacaa aacccgcact gcactgcgcc ggatttcgca taagcctgct
    11821 gaacatgaga aagttttccg cctttcgctt caccttccag cgtgcggatc tcttttcctt
    11881 cagcccaggc ggcaaggtat aagcaactgt ctattgctgt gccgtcgacc agcaccgtac
    11941 aggcaccaca ttcacccacg cagcaccctt gtttgacact tagcagtcct tgttcgcgga
    12001 gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg aaaaggcatc ccgttaatgg
    12061 tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat tttccccccg ccgcggcgac
    12121 ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa actctttact
    12181 ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca gattcgctga tagcttccag
    12241 cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc agcgaatcgg
    12301 cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt tatccagtcg
    12361 gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg tcgcgcatgg catatttaaa
    12421 atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga cgaggatttc
    12481 gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg
    12541 aacaccgcgc ggggagtgga tctccagttt cgcgtcataa attagcgttg gcgtggcaga
    12601 atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt tacggatctg
    12661 cggtccagca atggacgtgg ccgcagcaca taacgccggg agatgacgtt gagttatagg
    12721 atcttctatt agctgggtaa atgtcgttgc agagccgata cgtagcgagc catcttccgc
    12781 cagcgtaatt ccccgcagct ccgccagatt atgaatatca acaatatggc gataacggtc
    12841 attgtgatgg tggagctgaa tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg
    12901 gttgtcagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt aagaagcaaa
    12961 atcaaacatg atgttatcct caaatcaatc ctgccagatg gaactcttca tataaccgtt
    13021 ttggcgtcag cggcagtgta ttgattgcaa caccggtagc catcttcacc gcgttacgaa
    13081 tagcagcggc aacaggaatt attggtggct cacccagtga cttatgtccg tatgcggatt
    13141 gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg cagatccggc atggtcggca
    13201 ttttgtaatc cagcagattg gggttacgga ccacgccgct tttagcatcg atgatcatct
    13261 cttcaaatag cgcccagcca atgcccattc ccattccgcc gtgtacctga ccttctgcca
    13321 gcagtggatt aagaatatgc cctgaatcat gaacgttgag gatgcggttg atggtgactt
    13381 tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc ggtgggttag
    13441 tggtggtttt gatggagctt tcagcagaga gctgcccgcc gcgttcaggg tggtagaaag
    13501 cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca accagcacga
    13561 tatggccttt tatcagggtc agattcatcg ctgactgatg tagcatgact gcggcgtgag
    13621 cgatgatttt ctcttttaat aatagtgccg cactgcgcag cgcaggcgcg gcaacatagc
    13681 tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac gtcggtatct tgagttgaaa
    13741 taacgcgaac gtcgctgacc ggaaccccca cggtttctgc caccatttgc gagaagacgg
    13801 tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat
    13861 tcatcagaag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta gaggtgtagc
    13921 taaaacaggc gacgccaacg ccgcggcgca aattgccttg ctggttctgg cattctgcac
    13981 ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc aaccctgcgc
    14041 tgtaaatacg tttgcccgtg agcggattag catctccttc gcgggcggcg ttgcgtaaac
    14101 gaatttcaac aggatcaata cctaacgctg tcgcggcgtc atcaagcata gactcaacgg
    14161 caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg aggttggtat
    14221 agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg actttattcc
    14281 cccccgcaga agcgatggag tgcccgtgag atgcataagc gccggtgtta gacagaacat
    14341 ccagactata acctttcaat gttccgtcgc ggttcacgcc catttgcccg tcaatggtaa
    14401 aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa actttcaccg
    14461 gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg ctcttccagt acatcctgtt
    14521 tattaccaaa accgccaccg acaaacggtt tgatgactcg tacgcatgac cagggaatat
    14581 ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg
    14641 taattcgcga gtcatcctcc atccacgcca gcgatgttac gctttccatg tgacaatgtt
    14701 gaataacggg ggtctgatag tgcccctgta cctggtagtc ggcggcatcg attgtttgtt
    14761 ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt gccaccgtta tggattggtg
    14821 cagcgtcttc tgccagcgcc gcttctggcg tggtgataac gggtaattct tgccactcaa
    14881 tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg acgatggcaa
    14941 cggcgtcacc atgatgacga acatggcgag ttagcagtgc gcgatcggcg gtatcgcgct
    15001 tgttttcgtc aagtgtccag gcatgcccag cagtagcaaa tggaatatca ggcacatctt
    15061 cccaggtaaa aatcgccagt acgcccggca aacttctggc ttgttcatca ttaatactta
    15121 cggcataacc atgtgcgata gggctacgta catatttcgc ataacacatg cccgccataa
    15181 cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg acgcgcatgc
    15241 atgattcacc cgtagcggtt gcttcccgcg cttccattga aatcccctcg atttgaacat
    15301 ttggttctta tggcaaagat ttagtctaag caggcaagat ttacgccaga aacgacgata
    15361 agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa cacagatatg atacgaaaca
    15421 ggactctgaa taggggcagt gatacgtatc aatatgagtg gcgataaaat caatgtgata
    15481 aattgggatt attacgcatc aggatgggaa atagggtaaa aagtcaggaa gttcgatacc
    15541 tctacagcga atatttagct gatgatactt ccctttgcca aatgaaatta acctctttct
    15601 ctattaccac gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata
    15661 aggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc
    15721 tctgggtatc gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa
    15781 atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct
    15841 atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa ctggcgcgac tgaacggcat
    15901 ttccccccct tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag
    15961 cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc
    16021 atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac
    16081 gacagggaaa gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat
    16141 ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg
    16201 caaccagctg cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac
    16261 ggcttacgcc cataatgaca cgatgctagt aaataatggg caaagcgtga aggctgggca
    16321 aaaaatcgcc actatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg
    16381 ttaccgtgca acggcaattg atccgctacg ttacttgccg ccgcagggca gcaagccaaa
    16441 atgctgatgg cgaattaatc agcagtcagc agcgcggcac ttgctaagga gagcgtaagg
    16501 tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc
    16561 aagctctata cgagggttcg attcccttcg cccgctccaa acaaacatct accaacgtct
    16621 actaaagtct accaatcccc cgaatatcaa ggcctccagc gattttcctc gtctacaacc
    16681 gtctacagtc atctacctaa atctacacac agaagtgtat agtaatgcgt ataggacaaa
    16741 atcgtttttt gggggctata cacatgttgc tttcagacct tgctatccgt cgcgcgaagc
    16801 caaaagagaa agcctataca ctcaatgacg gcaacgggct ttcgttactc attgagccga
    16861 acggttcgaa gggctggcga ttgcgataca ggtttgcagg caagcccaaa atgttgtcgc
    16921 tgggtgttta tcctgaggtg tccctttctg atgccaggac ggcaagggat gaagccaaaa
    16981 ggcttatcgc tggtggagtt aatccgagtg aggtcagaaa ggcccaaaag cgagaacagg
    17041 cgaacaaatg cggtaacacc tttgaggcga tcgcccgtga gtggtatgaa aaacgcctgg
    17101 acagatggtc acaatcatat gccgcagaga tgattgatac gtttgagaag gacgtattcc
    17161 cgtatatcgg cagcaaaccg atagcagaaa taaaaccgat ggaattgctc gcggttctct
    17221 ctcgtcttaa tgaccgtggc gcaactgaaa aattgcgtaa ggttcgacag cgatgtggcg
    17281 aagtgttccg gtacgcaata attaccggac gcgctgaata taaccccgct cctgatctgg
    17341 caagcgcatt tgcaccacac aagaaagagc attacgcgta tctgaaagtt gatgagctac
    17401 cggagttttt ccgcgttttt aatacgtaca ccggaagcca gatcgtcaag ctgggtatgc
    17461 gattgttgat tcttacagga ctccgcccag gtgaattacg gcaggcagag tggcaggaga
    17521 ttgattttga taatcgtctg tgggaagtgc cgaaagaacg aatgaaaatg cgccgtcctc
    17581 attgcgtgcc actttcagaa caggcgatcg ctattctgga acagttaaaa cacatgacgg
    17641 ggcagtatcc gtttattttc cctggtcgaa tacagcacag taagccgatg agtgaaatgg
    17701 ctatgaacgt gcttattcgt cgtattggct atgcagggcg cgttactggt catgggttcc
    17761 ggcataccat gagtacgatt ttgcatgagc agggttataa ctccgcgtgg atagaaacgc
    17821 agctggcgca cgtcgataaa aactcgatcc gtggcacata caatcacgcg caatacctgg
    17881 acggacggca ggagatgctc cagtggtacg ccgactatat ggactcactg gaacagggcg
    17941 ggaatgtggt acatggcaag tttggaaaat gtggatgact ggttgagtat acagtagtag
    18001 actttgagcg actaaaaagg ctgatgcaaa ccgaactgga tcggccttaa cattagaccg
    18061 cttgaagggg gagaatatgc caattgtgga aaacattcct gacttatccc actggcgaac
    18121 agtacaggag ttttctatca cccaagccgc tttattgttg gccggaattg atccttatga
    18181 ttaccctact cttgaacatg ccagagatta taaacatgaa cgttggaaaa tggcatgggg
    18241 gttggctaat ggaatggtta ctgcaattcg tcgtggtgta ttaactcctg tgtggattga
    18301 atattatcct ttgtgggaag tacaaagagc atccggcacc ccccgtacag agataccaga
    18361 tcgtttgaag aaaatatcca tggacgaaac aattattacc cgtcattctt tacttgaatg
    18421 ggtaagaaat gagagggtcg attatgtaag acctcctcgc tctatatcgg ataaggagat
    18481 taagccatct aaccaatcag taataattga tgctgaatcg cgcgtgatta attctgaaga
    18541 aacagtattg ctgacttatg gtcataagag cgaaggtctt gaatatgtag aagacgcaat
    18601 aagagaactt tgggcaacat atgatccaga taatcaacaa acagccccta caagaaatga
    18661 tgttgttgaa tacctgaaag gcaggggagc ggggcagaac atggccgagg ctgtaaacct
    18721 gatactacgc cccactggct tgcagcaagt tggaagaagg aaaagaaaaa caaatagtta
    18781 aatagtgccc acccttcgta aaaaaggtgg cactatttgc tcaaaactct tttgtttatg
    18841 gcgagaaatc atagttaagc gtgcgttttc tttgcttatt gatactggaa tggtggcagc
    18901 attattaaaa taatagtggg cactattcca ttgaaatagt acccaccatt aaaaatagta
    18961 cccacctata aatatgtttt ccttttatgc atcattgtag acggttatag acgttacgag
    19021 gattgaatat gtcaaataac ttacttcgtc tttctgatgt tatgcgccgt actggttacg
    19081 gtcgcgcttg gatttatcgc ctgatcagcc tggggcgctt ccctaaacct gtaaaaattg
    19141 gttcccgctc cgtggctttt attgaaagtg aagtcgacga atggattaac cagcgtattg
    19201 atgcttctcg caacaacgcc gcgtaataat cttttgggag gggggtattc tcccccagca
    19261 ctaaacctga tccgacagca ggagttttca atgaaacgtc cgaatatgcc cgtacagggg
    19321 cagggcttca ttcagcctga aatcagccag aacgatatat tgattcgcca caaagcagag
    19381 tcgcgtattg attcccgcgt tatttctgag cgagcaggaa tacaacacga aagtatcatt
    19441 gcgacgatta aatctcatca atccagattg cgcgagcttg gctcactgcc tcgccagtca
    19501 gtcaaggaat taacctattt gaaatcggga aaatctggcc gcaagcgcgg tcgtcctgaa
    19561 attagttatt tgctgaatga gctacagctt gactatctgt tacgcgttat ccgtgggcgt
    19621 gacgcagaac gtatcaacca gttcaagctg gatgttacga aggcattcgc gaagcatcgc
    19681 gccgctgaac cagtccgtcg cgaatatctg ccaggctatc acgaaagccg tgacgggctt
    19741 aaagcactgg gcgcagaacg tcaccattac atcaaccttg cccgcactga aaatcgcttc
    19801 gctgggctgg ctgtaggcga gcgtcagagc gcgggagagc aacagttagg cttactgata
    19861 gtcatgcaga aaatcgagca ggcagcattt aacgaggcga tacagaacgg catgacaccg
    19921 accgaagcgg tgcgggaagt tgctcgccgc atggagacgt tcgccagcct gatgacgtca
    19981 gcgccgtctc tgggggtgaa tcatgcgtaa ttatgatttc gccgcaatta ttccggtatc
    20041 acctggcatt attggcagat atgaaacggg cgttgtcagc gcacgcgcac tgcataaagc
    20101 gctcggtgta ggccgtgatt tcaccacctg gattaaaggg cgtattgagg aatacggatt
    20161 taaaaaaggt gtggattttg aggctgtaga aaatttgtac tttgattccc cgaaacgggg
    20221 gagccaaagt gtaaatattg aacagtttga ttctggctgg cacgtcgcct gggagaacgt
    20281 gaacatgcct gacatccgca acgctgccgc cctgtacatc gatactgcgg ataagttcgc
    20341 acaggtgagc atcaaccatg ctgattacat gcgccatatc gtgagccatt tcgccgctga
    20401 gcgcatgaaa cacgttaagc attatcagaa ggaggcttta tcaaattatc gcgctggtgg
    20461 ccctgtacgg cgtgaatatc cgcatgatgc aggaatggca ggcaaaacaa aggcgaccac
    20521 gaaagggtcg ccagtggaga taaaggaaat caagcgcatc accaactatg ccatgagtgt
    20581 agctggtgga caaggctatc agccaggcgg tcggttttta gtggctttta accgccgtgg
    20641 gtatttctcg acaaaatctt caagggcaaa tcgtttttgt gggtgttttt tcactccggg
    20701 aagaggcgtt aactcttcgg gttctgatat ctctttagcg cgtttcagtt ccagttggcg
    20761 ctcaagtatg cggtgcaaaa gctgtcttcg ttgttcggca aaaatatctg taatggattg
    20821 gtactttttg ttcatgctgc tgccctgtaa agcaatgcgc cgtagtacct cacaccacgg
    20881 cgctgatagt gattattctg attctttggc cttgcggcgc tggcggcgtt tgatctcgcc
    20941 tcgcatggct gtaacgataa attgcgcagt gctctcgcct gtttctttta cttgctccat
    21001 agcctcaaca acttcatgcg gggcgcgagc ctgtaatttc tgtgatttgt tattgatatg
    21061 gtctctttcc atttctgctt ccctttgtga ttactggaat ccagtataca cgaaaaagac
    21121 caaaacaaaa cgcttgaagt ggattccact tagtgattat actggaatcc agctgagtgt
    21181 ttatcactca atgcaaacga ggctgaaaag gattgccgtc ctttcgcagc ctctgaccac
    21241 caacgatagc aagagtatcg aggcagctat gagaaatcat atcacacacc cgcaagggcg
    21301 ggactcgcac aacctgaata aattcacctg gcgctttatg cagtgcctga caggcagcat
    21361 tgcaatattc accgttaccg ctgccactga gcgcgaagca cgcgcccggc tgccgcacgc
    21421 acatctgatt tttgtcgccc gtatccgtca ggaggcatca catggataaa aacatgttga
    21481 tcgcctcttc tttcgggctg tatgatgccg aagtcgccca catggcgacc gggtttagca
    21541 gcctgaatca gatagcggac agccgcttac attccgatat gcggtttttt tgtgtccgta
    21601 agcctaccca tacccgcatt atggcggggc gtaacggggg agcctttgcg ctcgctggtt
    21661 cctatctggc cagtctgcta accccgttac gtctcgccac catgtttagc agcgtagtag
    21721 cgagactcct taatcaacag ataggagcct ttcacatggc tgtatccgca cgcacatctg
    21781 atttttgtcg cccgtatccg tcaggaggtg actcatgctt aaaaccttcc gcgtcttttc
    21841 ccgtgctgtt aacaagcgcg gctataccgt tggcatcacg cagaacgtga aggccgaaaa
    21901 cgtccaggct gcaatcagca cagtaattaa caaatcagct aaagccggtt tatccggcgt
    21961 aattatccac gcagtgacat gtattcagga tgaggagtaa ttcatgtgtg atttttccga
    22021 tatgactgtt gatgaagcag caaaaagcgc aatgcaggca gaacttattt gttctctgat
    22081 gatgggtaac acaggtgaaa tgactgaggg tgagattgag tcattgctcg ccctgattaa
    22141 gcagcttaca ggtaaggcag gtagctggct gattgctgca tcgggtgatg atgcaatatg
    22201 cgtcaatgac tgaatccttt gttgttctga atatcatgaa attaaggggg tgcagtaatg
    22261 cagcaaataa cactggcaga agcctcactg caagcgcaac aggtcgaagt aattagccgc
    22321 atgattgaac gcaaccacga agaaatagac gaccatgacc tttctgttat tgcaggactt
    22381 atcaaacaac tttccagcaa tgtggcggtg tggttaatga cggaggaaga aaaacgcggc
    22441 gaacaataac ggctaaaaat aaacgggaaa taaatattaa gacgactcct tcaggagatt
    22501 aattcaatag catcaaaaaa tactggctta actgccagta gcaaaactca ccccgaattt
    22561 atgtgcgggg atatttaccg caatgaatac ggtagccaca tgacgatgat cagcagaggt
    22621 aagaaatgaa aagcgcaccg aacttaaaaa aacagcctcg agggaaaaaa cacgccgata
    22681 cggaagtgat tattttcgct ggcagtgatg cgtgggcgca cgcgaaacag tggcaggagc
    22741 aggacggaaa acttgccgga gataaaacac cgcctgtcgt gctggctgat gagcaactta
    22801 aagatattgg caatctgcaa attgtgcccg acgggcgcaa atcagcgcgg atatacaagg
    22861 ccggacacct tgatcaggtg atggtcaaag gcataggtca gaagctggcg gcggcaggcg
    22921 tacaggacgc agactattac cccgaaggta tgcaccataa cgagcggcag aactggcgta
    22981 actatctgga gactgagcgc aaaaacattt ctgacggact ggttattgag ctgcctgtaa
    23041 agaaaaaaac atcagaacaa tcagaggatg aattaaaacc ccgcgttgaa tgccgtcctg
    23101 atggcgttta ctgggttacg ccaaaggttg ataagcaatc cggtgaaatt atccgcccgt
    23161 gtgagtggat tggtgaccgt atgcgaaccg tggggatcgg ccatgatggc tgcgatgggt
    23221 atctgataat cgaacttgaa ccagagggaa tggaaaaaat tatttatgag gccatgcccc
    23281 gcaatgaaat cggcctccct tctggatggt cgcgattgcg tgggcgtggg gtggcagtga
    23341 caaccagtca gaaactttta aacaaacttg cggaatacct tcagcgtgaa ggagagcgga
    23401 cacagtggga ggtcacctca acggcggggt ggcattgtgg cgcttatgtc atgccggatg
    23461 gtgagattat tggcgtacca gaaaggcccg ttgccttctg tggtggttcg gcggcgatta
    23521 aaggttatgt ggttcggggt acggctgatg agtggcgcga caatgttgcc tcactgatgc
    23581 gcggtaatca ctcaatgatg cttggagtgc tggtcgggct tgccgccccc ctgaactccc
    23641 ttgttggtgg cggctgtttt ggtgttcatc tttttgcgca gtcgtcggca ggaaaaacca
    23701 cgacagtgga agcagcaaca agtctttacg gtgatcctga agtgctgaaa ctttcatggg
    23761 atgcaaccag atacggcctc acggtggaag ctgcggcacg taatgatggc tttatcccca
    23821 ttgatgaaat cggtcagggc ggacggatta acgatattgc gcagtcagcc tacagcctgt
    23881 ttaacggtgt ggggcgtatt caggggcgta aggatggcgg taaccgtgcg gtgatacgct
    23941 ggagaatcgc ggccctgagc acgggagagg aggatttcga aaccttcctg attaagggcg
    24001 gtgtgacgcc taaagctggt caactggtgc gcctgttaag tgtgccgttt attgatacaa
    24061 cggtttttaa cggatatgac gatggtgatc agcacgccag agcaataaaa cggtttgcat
    24121 cgaattattg cggagcagcg ggtcgtgagt ggatcggctg gttgtcagcc aacaaagaac
    24181 ttgcggtcag tgtggttaac gaaaaagaaa acatctggct tagtcgtctt cctgaaaatg
    24241 cttcccccca ggtgaaacgc gtggcatccc gttttgcaat gctggatgca gccggagaac
    24301 tggcaacggg aataaccgga tggacaacgg gagaaagcca tgcagccaca cagcgcgctt
    24361 ttgatgactg gttgcaggat tttggactgg ataacaggga aaaatatcag gttatcagtc
    24421 gcgcccgcga tttcattcag cgtcacgccc ttagccgttt tcagccgtac acatacggca
    24481 gacagaacgg agatatggac gtgaattacg gagcgagaat caccagtctg gcggggtatc
    24541 tggtgagagg gcgacgtgat gacggattac cggaatacca catcataccg tcagtatttg
    24601 atgaagaaat cctgtgtggc attaaccgaa attttggatg ccaggcactg aaagaagccg
    24661 gaattctgat tcatgcaggt gataaaaact ggacgaccaa aacgataaag gttaacggaa
    24721 tacagcaacg ctttattgtg cttcttgacc agtcggagga gtaaaaatcg tattctcgtg
    24781 tacgcgtaaa aaagtgggcg gataagcgga tatggttaat aaagtgctta tatatcagag
    24841 cattatattg tatttccgta tccaccctta tccgcatata tccgccctgt tatgagagga
    24901 aattgataaa tatcaatctt gtgcaaaaaa cacacaaagt cgtatccgcc attatctgtt
    24961 tgattctgaa gagaaaaaac atacaaggcg gatatgggtg gatacgaaaa atcacgtaac
    25021 acaatgaatg caaacaatat ttttcttcat atccgcttat ccgcccaaaa agtgtcttta
    25081 taagtgaata tgtgaagtta aaaaacagag agcaaagcgg gagattaatg ccatttcccc
    25141 gcagtaacta cagagagaat catgtaatga gcaaagaaaa aatgaaaatc aggcagcacg
    25201 gcaggacaaa gatacttaaa ggtgaattta tgaactctga cattaaaacg ctgagcatat
    25261 caggcgccct tcctggctgg tgggcgaggt tcaaagatga cgacggtacg gaatggtata
    25321 gcccaattgc tgcatgggcg ctttgtgagg tcgccccctg taatacggga tgtgcgtatc
    25381 aggagatact acccgtcttg ccaggtgaag caggcatgga gccgcattat tctgattgtg
    25441 gggcgtgcga gtgcctttat ctgccagata agaagtttgt tcactgcggt gagtcatggg
    25501 tatttgcctg gtatccggtt aacgacggca gcaatagcgg tactctggag taaatcatga
    25561 aagtaagcca caaaaagaca gatcaccgcg ccattgacat tacagagcac tggctgaggg
    25621 tggcgataaa aatcattgac cgcaacacgg gagaaggata cgcgaaggaa catcccgaac
    25681 tgataagcgc attcatgacc acggcggcgg caaactttgc cacgctgaca gaacgggaga
    25741 ttgctgaagc ggaacaggta gcaaccatca acgttaaaac cggagaggtg gaatcatgac
    25801 agcacagatt gcagcttacg ggcggctggt ggacgacccg caggaaaaac agaccagcaa
    25861 aggaacacca atggcactgg cgcgtatggc ggtatctttg ccatgcagtc aggcacagga
    25921 cggacaggcg acgttatggc tatcagtcct ggcatttggt aagcaagccg acgcgctggc
    25981 gagacatgaa aaaggcgacc ttatcagcgt ggcgggtaac atgcagatca gccagtggac
    26041 cggacagaac ggagaaatac ggcagggctg gcaggttatc gctgacagcg taatcagtgc
    26101 ccgttcggcg cgtcctggcg ggaagcgggg gcaacagggg aaggcgaccg aagcactgga
    26161 aagagcaaaa cgacagaccg gacagcaggg aaatcagcca ccagcgggag gtgatgaccc
    26221 gttctctgac gatattccgt tctgaatgac aggagcacaa catgaaccag cagaccacac
    26281 aacaggacca gaccacacgt aaaccatcac ggcgcatacc gtggcagcac atgacaaaag
    26341 agcaacgaaa aaaccacaga cgaataaatc gaattgcgga atactggcct gagctgtttg
    26401 ggacagagaa tataaaaccc ctgaaggctg gcataatacg tgatgtgctt gaggacgcta
    26461 aatcacggga gttgccactt gctacaggtg tattacgcgg cgcattaacc tcacaccttc
    26521 acacgacgcg ataccttaag gcgattattg ctggtggccc acggtacgac ctgaacgggc
    26581 aaccttgcgg cgaagtcacg gagaaagaga aagcagcagc cagcgaacaa ctgatgtgga
    26641 ggctggagca gataaaacag caaaacatcc agtggcagga tacgggaggt ggtgagagtg
    26701 ataacagaca gcgtggcaca gaaacttgag gagcgtggtt tatggcgacg tgcggcgacc
    26761 cgatgctctg acgtccttct tcacgcagaa acggacaggg agcgcgagga agctgccaga
    26821 cgtcgggcga tatgcatcat taaatcccgt cgtatgcctg agcagttcgt cacctttggc
    26881 gatgtgaaaa aggccgcaga cagaacgctg aaagagatgg ggattaatcc gcaggatgaa
    26941 tggaaaaatt acagcttcag tgatgcaggc gacgatttag cgctatcgtg atacgtatct
    27001 catcgccgta tttgctcaat tatcgcgatt cgctataatc accaccgaaa ggccgttgat
    27061 ggtgttctcc gcaggcaacg ggagcctcgc cgaatgaagc cagccagcgg cttttcggca
    27121 ctcattttga tgtgtacttg tcacacacat cgggatgagt gaacgacgaa aacatcacgg
    27181 tgatttcgtc atgccgcgat aagaatgcgc ctctttgggc aactggagcc ctctgtacag
    27241 cgggttattg cggcaaagtt gtgataaaaa cgcgcctctt tgagcaattg aagctcactg
    27301 taaagcggct tatcacagca ggctgtaacg ggcgttcatc ttctggcaac tgaagccacc
    27361 tgtacagaga cccgttacgg ctgaccctga aggatttcgc cccgtgcaat tgaagcaccc
    27421 agcggagtta ttcagggttt tttattgccc gtccaccagc accacatctg tagcattgat
    27481 gcgtatacat tcgccgtcaa ttgcaccgtt ccggccctca ccgtcacgca ccacaacaga
    27541 atgaccaggc aggccataac gcgccacctt tcgacgaaca ccatcacctg ttccctcacg
    27601 ccgctcatac acgttaaccc gctccagaag accgtcacgg accatcgctt caagtgtgcg
    27661 ccttgttgat tcgagctgat atcggttgtt gtgtgaatct atgccataca gcaggtaagc
    27721 cacaccggag acatcaaacg gcgggttacc aatttcacct ctgacccacg acagattgtc
    27781 aggagtgaag taactgagga tctctttttt gtattgcgtc agtctcatag tgacctttct
    27841 tgttgtagga tgaatccctc atacaataag ccataaggca aattttgaaa ggacagaatc
    27901 ggcggggtac agatgatacg tgatgacaag gctgaagcgc tggagaatag cggcctctgg
    27961 cggcgtgctg ctgaacgctg gggagaattg ttcagacagg caaaaagtga tgaagagagg
    28021 cactatatta cccaacgaag ggcacaatgt acggcaaaag ccaccagacc aaaaacaaca
    28081 gagtgggggc atgtatgcgc cgtcagggca gcagcgacca gaacgcttaa agatatgggg
    28141 attgaccaga aaaaagaaga tcctttacgg ggcacgaagt acaggggtga aacagggcga
    28201 agagacggaa gttaatatgg acacaataac tgataaaaaa gccgaacaac tggaatctca
    28261 gggactatgg cgaagagccg ccgctcgttg gcttgacgta atgaaagagg cccacaccga
    28321 tccgcagcga gagcatatag ccagacggcg ggaaatatgc cttgcgaact tcaggatgct
    28381 gtaaataaga ctgttgacga tatgggaatc agacgggatc ggcgacatta cgggttatgg
    28441 aaataacagc caggtaaatt ggtgaatata cggcaggctg caatcagagc cgaacatagc
    28501 ggcctccgtc accattatca gacagtagcg gtagtccttc catcacagca aaatgctacc
    28561 cacttcacag ccccttagaa gcctcttcga tggggtgaaa aatccctgaa aaaatgcgca
    28621 aaaaactgcg caaatcgatc ataaaaccga tcacaaaatc gatcacacag accagcagag
    28681 caaagccaga tatgacgtgg cttgtagggg gaaaagccac cggaaaaagt gcgcattttt
    28741 ctcaaaaacg aaacgcaaca ggaagtgcaa aacgaaaccg atacgcgatg atcgaaaaag
    28801 cgcgcaaatt taccgggttc gggtaagcaa ttttgcctga tttgggcaaa atacggtgtc
    28861 gccgtaacag tgacattgac accaaaacag tgacacacag gagggccgat gaccgaacgt
    28921 tcaccaacag agataattca cgggattatt gaagagcagg aggaacgcga gttaatcctc
    28981 atgattgaag agcagagaga acgccagcgc aaacaggagg ccacgcagcc ggacagcgtg
    29041 agcgctgaaa atcaggctga tgtgacgtga tggtgtcgaa ctgagtagct ttaagcgact
    29101 ctgtgactgg ttttaaacga gtcgcaaaaa gagccggaga aaaaccacga atctggggtt
    29161 ttttctaagc acgctgtaca cggtggaaca tatggtggtc aggagacggc aggagcaacg
    29221 ccagaacgcg cccccgtacc agtggcataa gccggagcgg ttacggcggt aacttgttga
    29281 ttaaaaggcg gctcaattct gagccgggta gctgacggcg ttaactggct gatattgtta
    29341 caaagctcat ttttgagctt tggcctaacc tactgatatt ttcggaaacc tcaatttgag
    29401 gagaccgcaa acgcaaaatt gcgctggcta cattcctcac ttctgaggac tgttaacaag
    29461 ctggctgaaa gacagtcaca agctacgaaa ttttcgtagt ttaactttca tccagtgcat
    29521 ccctgaatta cgggtatgta accacagcta ttacgaggtg attgttgatg cgtgatattc
    29581 agcaggttct ggaacgctgg ggagcgtggg cggcaaataa tcgcgagaat gtgacctggt
    29641 catccgttgc cgcaggattt aaggggatta ttccggcaaa gataacatca cgctcacagt
    29701 gctgtgacga tgatgcgatg gtgatatgcg ggtgcatagc ccgtctttac cggaacaatc
    29761 gcgatctgca tgacttgctg gttgattatt acgtgttggg ggagacgttc atggcgctgg
    29821 cacggaaaca tgggtgctct gacacctgta taggtaaacg ccttcacaaa gcggaggggg
    29881 ttgttgaagg catgttgatg atgctggatg tgaagctgga gatggatcgg tatgttgagc
    29941 gcgattcagt gacggcataa cggcataaaa aaaccgcagt catgaggaaa ctgcggtgga
    30001 gaaatacggc ttgccaatca atcatgaaaa acgtaatcgc ctgataatta tgccacgaga
    30061 caaaggacgg tgcaccctga atggtcataa tgaccactca gaccatcgag gcaaccataa
    30121 cgaacatcgt tacagttgga gcttttaccc cgttggggga aaaggtgcat acctgaatca
    30181 tcattatggt gatcaagggc atgtaaagcc caccagcctg attaaaggtt aaccggaaaa
    30241 agccaggtat ggagggagtg attagccacg aacaagccgg acaccaccag cagaaggtat
    30301 tatgattttc gtaatacctt tagcaaagcg tgaatcatca ttaccgtagg gatggtcata
    30361 atgaccacac cttcccccac cggaacaagg aaggctggct tacgaaaatt tcgtaaccct
    30421 ccccagcaca atgtattaaa taatcactac aggccattga ttaaacttaa ttatcgcgaa
    30481 tcgcgagaat tagccccgcc ggaacaagga aagggaaatt acgaaaattt cgtaaaatcc
    30541 ccccacatct cccacggttg cccgttaagt gcaagcgcac aggcacgacc tcattaacca
    30601 gttaccagcg ctcacgctca aacatccaca gcaatgggga taaatcgcac tctggcgcaa
    30661 tgttcactat cggggcgagc attcaccgga aaacgtcata caggcttaca ggcggcttaa
    30721 aattcgatgg gcggaggacc ggatgggaat cgggagacct ggacaaaaag tgcaggcctc
    30781 catgacacgg tcaggacagt ttgtgtttaa ccagaatatt tagcgcctgt attggggctt
    30841 cgtggagctc ccgcagacat tcagggtcag taacatgttc ctggtatctt gttttctcaa
    30901 gtaacaagcg gtgcatatgc agcatacggg caagagccgc gcacacctcc tcaaatgcgg
    30961 tatcccgctt accgtcactg tcagagccac aaaacagcgg tacaacctcc acgccgtcgc
    31021 tgtgggcctt tagccaggca tcaaccacgg ttaaatcatc ggtgaagaac cacgcactgt
    31081 tagcgctgaa tcgcattttc caggcttccg gtactggttg cggggagtat gaatatgctg
    31141 gtggtatctt gtcgtgagta tccataggct atcgttccgt cattctgtta ttcaccacag
    31201 cgctaaaaac gccatgaagg agcagaatac cgattgcctc gtttgctgta tgaggtgctt
    31261 accacacatt gggtacacag atgaatcagg cggaagtgtg attaccggaa aaaatccatt
    31321 tcagtttgag ggaaaaaaac cgtagtgcga taacactacg gcatgccacc aaagaaacac
    31381 aacaagaggt caacaatgag taatggacat gaccagacga ttttatcatg ccggaacaaa
    31441 agttatgtga cacagtcgga taaaaacagg taaagcccac cagcttcata ggggataacc
    31501 ggaaaaattc cggtaaatat ttgcctgagt aagtcatcaa gcgcctgcta aatgtgtata
    31561 gaaatgtgta taggattgat tttagaacat gtggaaatgg ctttattact ggtggcatgg
    31621 cttgttgttc tgttcccttc gccgctccaa tttatcttca cctgatcaat ctgccatttt
    31681 tgctgtatct gattttctat attatcttaa ttcatcgctt cagacttttc gcaatcgccg
    31741 taatacttcc ccattttgtt gtaaataaaa agcgtttacg caatgcaatc cttttgtaac
    31801 aacccaggta aaacacctta ttttcaagtt gttaaataag tggttatcta cgcttttaac
    31861 aaacgattga tggaatattt tatgaaaggg aaatctgcat taactcttct gctcgctggt
    31921 atattttcgt gcggcacatg ccaggcgaca ggggcagaag tgacgagtga atctgtattt
    31981 aacattctta attccacggg ggcagcaaca gataaaagtt atttatcgtt aaatccagat
    32041 aagtatccta attatcgtct tttgattcac tcagccaaat tacaaaatga aataaagagc
    32101 cactatacca aagacgaaat tcaaggattg ctaacattaa cagaaaacac acgaaagtta
    32161 acgttgacgg agaaaccctg gggaacgttt attcttgctt ctactttcga agatgacaaa
    32221 acagcggcag aaacccacta tgatgctgtc tggctgaggg atagtttatg gggctatatg
    32281 gcattagtat cagatcaggg aaatagtgta gccgcaaaaa aggttctgct caccctgtgg
    32341 gattatatgt ctacgcccga ccagattaag cggatgcagg acgtcattag caaccccaaa
    32401 cgactggatg gagttcccgg gcaaatgaat gttgtgcata tccgatttga cagcaactcc
    32461 cccgtgatgg cagatgtgca ggaagaaggc aagccgcagc tgtggaacca taaacaaaat
    32521 gatgcgttag gtctttatct tgatctcctt atacaagcaa tcgacactga tacaattaac
    32581 gctgaagatt ggcaaaaagg tgaccgatta aaatcggttg cgctgctgat tgcttatctg
    32641 gataaagcca atttctatgt tatggaagat tccggagcat gggaagaaga tgcccgatta
    32701 aacacatctt ccgttgcact ggtgacctct ggactggaac gtttgtcgaa cttactctcg
    32761 aagaaagact ctgtttttgt ctctgattta ctacgcgaag ctaaagccaa tgaactggat
    32821 gaacctcttt caacaacgag gttgaaccac ctcatagata aaggttatga aagaataacg
    32881 ttacagcttg atttaggggg agaatcccca ggttatctgg aaaaagataa acattacaga
    32941 gaagccgatg cggctttact taatgttatt tatcctgcaa atcttgcaaa gataaacacc
    33001 agaagaaaag agcaggttct aaaaatagtt aaaaaactag caggtccgta tggtataaaa
    33061 agatacgaga aggataatta tcagtcggca aatttttggt tcaatgatat taaaaccgat
    33121 accgatcaaa attcacatgc gaaaagagaa aagagcttca ttccatcaac agaagcagaa
    33181 tggttttttg actcatggta tgcaaaatca gcagcaattg tttataaaga atcaagaaag
    33241 gaagagtatt tgaatgactc agtgcaattt atgaatagat cattggccca aataacaggc
    33301 gagaatatga ttggtgcaaa tggcaggagt gtacctgaaa tggcacttcc cgaaagctat
    33361 aactatatcc acaagtcggt aactttgcat gaagcgccaa gccctatcat tccgctaaat
    33421 tggtcgaaag ctt
//
LOCUS       DAZFVO020000039        33258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFVO020000039 DAZFVO020000000
VERSION     DAZFVO020000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 33258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.039"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..96
                     /locus_tag="ACHKNB_RS08690"
                     /old_locus_tag="pgaptmp_001738"
     CDS             <1..96
                     /locus_tag="ACHKNB_RS08690"
                     /old_locus_tag="pgaptmp_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4195269.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            complement(137..1495)
                     /gene="dgcT"
                     /locus_tag="ACHKNB_RS08695"
                     /old_locus_tag="pgaptmp_001739"
     CDS             complement(137..1495)
                     /gene="dgcT"
                     /locus_tag="ACHKNB_RS08695"
                     /old_locus_tag="pgaptmp_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4195270.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            2082..4505
                     /gene="pgaA"
                     /locus_tag="ACHKNB_RS08700"
                     /old_locus_tag="pgaptmp_001740"
     CDS             2082..4505
                     /gene="pgaA"
                     /locus_tag="ACHKNB_RS08700"
                     /old_locus_tag="pgaptmp_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HGU4195271.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYNHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            4514..6532
                     /gene="pgaB"
                     /locus_tag="ACHKNB_RS08705"
                     /old_locus_tag="pgaptmp_001741"
     CDS             4514..6532
                     /gene="pgaB"
                     /locus_tag="ACHKNB_RS08705"
                     /old_locus_tag="pgaptmp_001741"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HGU4195272.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVYPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            6525..7850
                     /gene="pgaC"
                     /locus_tag="ACHKNB_RS08710"
                     /old_locus_tag="pgaptmp_001742"
     CDS             6525..7850
                     /gene="pgaC"
                     /locus_tag="ACHKNB_RS08710"
                     /old_locus_tag="pgaptmp_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HGU4195273.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLTTTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            7852..8265
                     /gene="pgaD"
                     /locus_tag="ACHKNB_RS08715"
                     /old_locus_tag="pgaptmp_001743"
     CDS             7852..8265
                     /gene="pgaD"
                     /locus_tag="ACHKNB_RS08715"
                     /old_locus_tag="pgaptmp_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HGU4195274.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(8315..9103)
                     /gene="phoH"
                     /locus_tag="ACHKNB_RS08720"
                     /old_locus_tag="pgaptmp_001744"
     CDS             complement(8315..9103)
                     /gene="phoH"
                     /locus_tag="ACHKNB_RS08720"
                     /old_locus_tag="pgaptmp_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HGU4195275.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(9722..10993)
                     /gene="efeB"
                     /locus_tag="ACHKNB_RS08725"
                     /old_locus_tag="pgaptmp_001745"
     CDS             complement(9722..10993)
                     /gene="efeB"
                     /locus_tag="ACHKNB_RS08725"
                     /old_locus_tag="pgaptmp_001745"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HGU4195276.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(10999..12126)
                     /gene="efeO"
                     /locus_tag="ACHKNB_RS08730"
                     /old_locus_tag="pgaptmp_001746"
     CDS             complement(10999..12126)
                     /gene="efeO"
                     /locus_tag="ACHKNB_RS08730"
                     /old_locus_tag="pgaptmp_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HGU4195277.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(12184..13014)
                     /gene="efeU"
                     /locus_tag="ACHKNB_RS08735"
                     /old_locus_tag="pgaptmp_001747"
     CDS             complement(12184..13014)
                     /gene="efeU"
                     /locus_tag="ACHKNB_RS08735"
                     /old_locus_tag="pgaptmp_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HGU4195278.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(13557..15065)
                     /gene="putP"
                     /locus_tag="ACHKNB_RS08740"
                     /old_locus_tag="pgaptmp_001748"
     CDS             complement(13557..15065)
                     /gene="putP"
                     /locus_tag="ACHKNB_RS08740"
                     /old_locus_tag="pgaptmp_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HGU4195279.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(15224..15433)
                     /locus_tag="ACHKNB_RS08745"
                     /old_locus_tag="pgaptmp_001749"
     CDS             complement(15224..15433)
                     /locus_tag="ACHKNB_RS08745"
                     /old_locus_tag="pgaptmp_001749"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195280.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            15488..19450
                     /gene="putA"
                     /locus_tag="ACHKNB_RS08750"
                     /old_locus_tag="pgaptmp_001750"
     CDS             15488..19450
                     /gene="putA"
                     /locus_tag="ACHKNB_RS08750"
                     /old_locus_tag="pgaptmp_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4195281.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLAHQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(19490..20128)
                     /gene="rutR"
                     /locus_tag="ACHKNB_RS08755"
                     /old_locus_tag="pgaptmp_001751"
     CDS             complement(19490..20128)
                     /gene="rutR"
                     /locus_tag="ACHKNB_RS08755"
                     /old_locus_tag="pgaptmp_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HGU4195282.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            20416..21507
                     /gene="rutA"
                     /locus_tag="ACHKNB_RS08760"
                     /old_locus_tag="pgaptmp_001752"
     CDS             20416..21507
                     /gene="rutA"
                     /locus_tag="ACHKNB_RS08760"
                     /old_locus_tag="pgaptmp_001752"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HGU4195283.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEETLSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            21507..22199
                     /gene="rutB"
                     /locus_tag="ACHKNB_RS08765"
                     /old_locus_tag="pgaptmp_001753"
     CDS             21507..22199
                     /gene="rutB"
                     /locus_tag="ACHKNB_RS08765"
                     /old_locus_tag="pgaptmp_001753"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HGU4195284.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            22211..22597
                     /gene="rutC"
                     /locus_tag="ACHKNB_RS08770"
                     /old_locus_tag="pgaptmp_001754"
     CDS             22211..22597
                     /gene="rutC"
                     /locus_tag="ACHKNB_RS08770"
                     /old_locus_tag="pgaptmp_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HGU4195285.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            22605..23405
                     /gene="rutD"
                     /locus_tag="ACHKNB_RS08775"
                     /old_locus_tag="pgaptmp_001755"
     CDS             22605..23405
                     /gene="rutD"
                     /locus_tag="ACHKNB_RS08775"
                     /old_locus_tag="pgaptmp_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HGU4195286.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            23415..24005
                     /gene="rutE"
                     /locus_tag="ACHKNB_RS08780"
                     /old_locus_tag="pgaptmp_001756"
     CDS             23415..24005
                     /gene="rutE"
                     /locus_tag="ACHKNB_RS08780"
                     /old_locus_tag="pgaptmp_001756"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HGU4195287.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            24016..24510
                     /gene="rutF"
                     /locus_tag="ACHKNB_RS08785"
                     /old_locus_tag="pgaptmp_001757"
     CDS             24016..24510
                     /gene="rutF"
                     /locus_tag="ACHKNB_RS08785"
                     /old_locus_tag="pgaptmp_001757"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HGU4195288.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            24531..25859
                     /gene="rutG"
                     /locus_tag="ACHKNB_RS08790"
                     /old_locus_tag="pgaptmp_001758"
     CDS             24531..25859
                     /gene="rutG"
                     /locus_tag="ACHKNB_RS08790"
                     /old_locus_tag="pgaptmp_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HGU4195289.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVSSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(25942..26115)
                     /gene="ymdF"
                     /locus_tag="ACHKNB_RS08795"
                     /old_locus_tag="pgaptmp_001759"
     CDS             complement(25942..26115)
                     /gene="ymdF"
                     /locus_tag="ACHKNB_RS08795"
                     /old_locus_tag="pgaptmp_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4195290.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            26884..26957
                     /locus_tag="ACHKNB_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /pseudo
     CDS             26884..26957
                     /locus_tag="ACHKNB_RS08800"
                     /old_locus_tag="pgaptmp_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(27046..>33258)
                     /locus_tag="ACHKNB_RS08805"
                     /old_locus_tag="pgaptmp_001761"
     CDS             complement(27046..>33258)
                     /locus_tag="ACHKNB_RS08805"
                     /old_locus_tag="pgaptmp_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4195291.1"
                     /translation="APTAEELVRSQMAEGDAGPTAQELTERPRLPAPGDIHPGQGYPL
                     PGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGRAQAPENAAGRQGEILEGEPVRRGL
                     PSPDAQNATAPVREGLPAPESQRGVDMPQPESLPRMVRDSLPELAQQAEARRQTGDRQ
                     TITDVPDTEVPVDKPSTHQQARGAKIEDFGEEIKGAAKHRYAQLAEAMGKTLEDGEYA
                     TQPLSKLFPKPDYARLEKEGVDSDTLAMMALYRSEIPTRTSRNMQKWISIVKSGREAT
                     AGMLEGKIPAAKLADMMDSKPGLRSMSDTWKLLRTLSALQIDKASGYRVRSGVYSFVG
                     GKRYDPPQMMYSLRDSKGRDLFFSESRDELLKKAKAYFDEQGSRERETPATSADDRIT
                     FDVYRHKASGDIFIGYGKNRQKLKGGFESARDAHDYVRTHRDELVNQVKALREVSREE
                     QRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYVESPRRQADLNRAYDSLHDL
                     ADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAINLTKGNGPGALAHEWFHS
                     LDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDAEYPVRQEVYDAFKGVMK
                     AINSSDMLRRSERLDGVRSKPYWSTDVEMAARAFERYVQDKARMAGVENDYLVNIRKA
                     DDHGQPDTYAYPTNAELDGGIREAFDHLFRTLKTRETDKGVAFYSRKGVTRTPEGNLI
                     SDVNRSAEAKGSPVSQVEAVARGVMSGIKDSDLKVRVVKSQKEAEALAGESFDGYGRV
                     HAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRD
                     SDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRR
                     TGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGVGASSQYSRSGKPDPFKVPEGE
                     GERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHV
                     VPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHMKATGKRLEI
                     NKVASVYGTENGKKLKSMEMAGLTLYRKEKLSRDNPQYSGLQLPKEERSYRGSVDKIL
                     YPEDIRKGPYYSRTSSLTPEEIIISRFVRQMQDKFRVLKAVQENIRKTGGKIDDSNNA
                     YMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIAK
                     INPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLEE
                     SGVVDAWQNAYRYYVPLKGQDVDGMVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQ
                     AIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRAIAERVDPETGE
                     TRREVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKSMGPETSNAFVRTLG
                     KVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVAKD
                     SRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVS
                     LAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMT
                     VNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEGPLLERLRWKNLNVPQKI
                     ALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSVFGGKAGEYWSI
                     PLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPVGSETSETLSGALLK
                     NAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNA
                     FSGGSQYRPGAVDITPESLKFWIDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPFL
                     GKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDT
                     RKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKVAVDRFNREYNKKVGVE"
BASE COUNT         8083 a         8844 c         8123 g         8208 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa caagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcaaaat gccgctttat ggtgactcac aaaaattcac cacatccctc acaaccacct
      181 tattacgccc ggtctctttc gcctcgtaaa gtgcgttatc agccagtcga tagatctcat
      241 tcgggtttaa cgcattttcc tgagtcacaa cagcgccaat actaatcgtc actttttgtg
      301 gtatagcgta ttcaggatta tcgccagtta aacgctcaac attttcccga atcctttccg
      361 ctaaagcttt cgcacgttca gtatcgatgt ccgtcagcaa gacgccaaac tcttcgccgc
      421 cgacgcgcgc taaaaggtca tctggtcgta tgcttttccc aatgatattc accactgttt
      481 ttatcacctg atcgccaacc ggatgccccc aggtgtcgtt gacttttttg aagtggtcga
      541 tatccataat catgacgcaa taaggcgttt ttttggctga tgctgattga actgtcagtt
      601 cattaaaaaa ataatttctg ttaaatatat tggttaaggg atcgcgatgt gcaatgttct
      661 ttgttactcg tagcgcactg aatatatggc acataaatat taccataacg gttaacttac
      721 tgactacttc aatagtgcga ctaatatacc aagtggacac gctataccta ctcataaata
      781 acagagagat attgtacagt acggcacaaa aagcaatcac cgtcactcca ttccagatat
      841 catagcgtaa accattaaag tatagattaa ccgaaagcaa ggttaaccac atgaaaatca
      901 ttatggtaac atatgaggct ttccaaacca cctgaccatt ttcattggtt aactcggcaa
      961 tgtgtaaatt gtagctctca taatgactag aaagtatgtg agcaactatc ggccccccaa
     1021 aaacaaaaaa caaacttatg cacaaagtca tttttttgga aaataaattg cgctgttttg
     1081 tgttttcact aactttattc accaatgcca gaaatattaa tatgcacaaa ctcatctgac
     1141 gaaacaaata atagattgca atgtcatttt ggacaacact gcttgtcaaa cgcccctcaa
     1201 taatttgctg aatgataatg acggttttaa caaagtaaat caaacaactc agaaaagcca
     1261 aactaagtat acagtttgaa aattgcctac ggtcataaat gaaatacaac tgcataaaaa
     1321 taaatgcaac agtatcgata aaaaataaag caatcattac catcagatag gttgatcgtg
     1381 gaacaacttc ctcaacgcct ggctgattaa accaactatt caccaaaacc aaagcaagcc
     1441 caaacaataa gctcactaat acagtactac taattctcaa atagtctttt tccataaagc
     1501 tacaccaatc tgtaggttgt agatcccttt ctattattaa tagatataag aactgtactg
     1561 ttgtcaatat gaaattccgc ccacaaacaa taccgatgat gatagttgct gaatgtaatt
     1621 attattgcct aagtgcttta tacattgttt gcaacaagcc aggcttgaaa atacttataa
     1681 atggttatag aaaatcctta taaaacatta attcatagat acattaggaa taacaattaa
     1741 atccgtgagt gccgtagcgc agcctttcat caggactttc gaaaaatccg aaatcatgca
     1801 tcggaattta ctgatttaat tattttaatc ctaatttatt ttgaaaaagg cattgggatt
     1861 tatgccgtat tcctgaagat cctcatcatt ggaatggatt ttcgggcgag aaaaggattt
     1921 tatatggaca ctctgctcat catttcttct tctcatcatc aacaattcac gtctctcttc
     1981 cgcgtttaat aacggattat gaggtgcaaa aatatctttc ttttcagtta cctgtaatta
     2041 gatacagaga gagattttgg caatacatgg agtaatacag gatgtattca agtagcagaa
     2101 aaaggtgccc gaaaaccaaa tgggctttga aacttcttac tgccgcattt ttagcagcga
     2161 gtcccgcggc gaagagtgct gttaataacg cctatgatgc attgattatt gaagctcgca
     2221 agggtaatac tcagccagct ttgtcatggt ttgcactaaa atcagcactc agcaataacc
     2281 aaattgctga ctggttacag attgccttat gggccgggca agataaacag gttattaccg
     2341 tttacaaccg ctaccgtcat cagcaattac cagcgcgtgg ttatgcagct gtcgccgtcg
     2401 cttatcgtaa cctgcaacaa tggcaaaact cgcttacact gtggcaaaag gcgctctctc
     2461 tggagccgca aaataaggat tatcaacggg gacaaatttt aaccctggca gatgctggtc
     2521 actatgatac tgcgctggtt aaacttaagc agcttaactc tggagcaccg gacaaagcca
     2581 atttactcgc agaagcctat atctataaac tggcggggcg tcatcaggat gaattacggg
     2641 cgatgacaga gtcattacct gaaaatgcat ctacgcaaca atatcccaca gaatacgtgc
     2701 aggcattacg taataatcaa cttgctgccg cgattgacga tgccaattta acgccagata
     2761 ttcgcgctga tattcatgcc gaactggtca gactgtcgtt tatgcctacg cgcagtgaaa
     2821 gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata cgctgcatta gaaattctgt
     2881 ggcacgataa cccagaccgt actgcccagt accagcgtat tcaggttgat catcttggcg
     2941 cgttattaac tcgcgatcgt tataaagacg ttatttctca ctatcagcga ttaaaaaaga
     3001 cggggcaaat tattccgccc tgggggcaat attgggttgc atcggcttat ctcaaagatc
     3061 atcagccgaa aaaagcacag tcaataatga ccgagctctt ttatcacaag gagaccattg
     3121 ccccggattt atccgatgaa gaacttgcgg atctctttta caaccacctg gagagtgaaa
     3181 attatccggg cgcgctaact gtcacccaac ataccattaa tacttcgccg cctttccttc
     3241 ggttaatggg cacgcctacg agcatcccga atgatacctg gttacagggg cattcgtttc
     3301 tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc tgaaatgaca gccagagagc
     3361 ttgcttataa cgcaccagga aatcagggac tgcgcattga ttacgcgagt gtgttacaag
     3421 cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa agcagaagtg atcgagccac
     3481 gtaatattaa tctggaggtt gaacaagcct ggacagcatt aacgttacaa gaatggcagc
     3541 aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac
     3601 gattaaaacg tgcggttgat gtacataatc ttgcagagct tcgtatcgct ggctcaacag
     3661 gaattgatgc cgaaggcccg gatagtggta aacatgatgt cgacttaacc accatcgttt
     3721 attcaccacc gctgaaggat aactggcgcg gttttgctgg attcggttat gccgatggac
     3781 aatttagcga aggaaaaggg attgttcgcg actggcttgc gggtgttgag tggcggtcac
     3841 gtaatatctg gctcgaggca gagtacgctg aacgcgtttt caatcatgag cataaacccg
     3901 gcgcgcgcct gtctggctgg tatgatttta atgataactg gcgtattggt tcgcaactgg
     3961 aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa tggtgttaca ggcaacagtg
     4021 ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt
     4081 tcactgattt ttccgacagt aaccagcgtc atgaagtctc acttgagggt caggaacgca
     4141 tctggtcttc accatatttg attgtcgatt tcctacccag tctgtattac gaacaaaata
     4201 cagaacacga taccccatac tacaacccta taaaaacgtt cgatattgtt ccggcatttg
     4261 aggcaagcca tttgttatgg cgaagctatg aaaatagctg ggagcaaata ttcagcgcag
     4321 gtgttggtgc ctcctggcaa aaacattatg gcacggatgt cgtcacccaa ctcggctacg
     4381 ggcaacgcat tagctggaat gacgtgattg atgctggcgc aacgctacgc tgggaaaaac
     4441 gaccttatga cggtgacaga gaacacaact tatacgttga attcgatatg acattcagat
     4501 tttaaggata aatatgttac gtaatggaaa taaatatctc ctgatgctgg tgagtataat
     4561 tatgctcacc gcgtgcatta gccagtcaag aacatcattt ataccgccac aggatcgcga
     4621 atctttactc gccgagcaac cgtggccgca taatggtttt gtagcgattt catggcataa
     4681 cgttgaagac gaagctgccg accagcgttt tatgtcagtg cggacatcag cactgcgtga
     4741 acaatttgcc tggctgcgcg agaacggtta tcaaccggtc agtattgctc aaattcgtga
     4801 agcacatcga ggaggaaaac cgctaccgga aaaagctgta gtgctgactt ttgatgacgg
     4861 ctaccagagt ttttataccc gcgtctaccc aattcttcag gccttccagt ggcctgctgt
     4921 atgggccccc gtcggcagtt gggtcgatac gccagcggat aaacaagtaa aatttggcga
     4981 tgagttggtc gatcgagaat attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc
     5041 ccggctcgtt gagctcgctt ctcatacatg gaattctcac tacggtattc aggctaatgc
     5101 caccggcagc ttattgcctg tatatgtaaa tcgtgcatat tttactgacc acgcacggta
     5161 tgaaaccgca gcagaatacc gggaaagaat tcgtctggat gctgtaaaaa tgacggaata
     5221 cctgcgtaca aaggttgagg taaatccaca cgtttttgtt tggccttatg gcgaagcgaa
     5281 tggcatagcg atagaggaat taaaaaaact cggttatgac atgttcttca cccttgaatc
     5341 aggtttggca aatgcgtcgc aattggattc cattccgcgg gtattaatcg ccaataatcc
     5401 ctcattaaaa gagtttgccc agcaaattat taccgtacag gaaaaatcac cacaacggat
     5461 aatgcatatc gatcttgatt acgtttatga cgaaaacctc cagcaaatgg atcgcaatat
     5521 tgatgtgcta attcagcggg tgaaagatat gcaaatatca accgtgtatt tgcaggcatt
     5581 tgctgatccc gatggtgatg ggctggtcaa agaggtctgg tttccaaatc gtttgctacc
     5641 aatgaaagca gatattttta gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa
     5701 catctatgcg tggatgccgg tattaagctg ggatttagat cccacattaa cgcgagtaaa
     5761 atacttacca acaggggaga aaaaagcaca aattcatcct gaacaatatc accgtctctc
     5821 tcctttcgat gacagagtca gagcacaagt tggcatgtta tatgaagatc ttgccggaca
     5881 tgctgctttt gatggcatat tgttccacga tgatgctttg ctttcagatt atgaagatgc
     5941 cagtgcaccg gctatcacgg cttatcagca agcaggcttt agcgggagtc tgagcgaaat
     6001 tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt aaaagtcgtg cgttaactga
     6061 cttcacttta gaacttagtg cgcgcgtaaa agccattcgc ggtccacata ttaaaactgc
     6121 acgaaatatt tttgcacttc cggtaataca acctgaaagt gaagcctggt ttgcacagaa
     6181 ttatgctgat ttcctaaaaa gctatgactg gaccgctatt atggctatgc cttatctgga
     6241 aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa
     6301 catccctcag gctaaagaca aatctatttt agaattacag gcacaaaact ggcagaaaaa
     6361 tggtcagcat caggctattt cttcgcaaca actcgctcac tggatgagcc tattacaact
     6421 gaatggagtg aaaaactatg gttattatcc cgacaatttt ctgcataacc aacctgaaat
     6481 agaccttatt cgtcctgagt tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg
     6541 tatcgttttt tatattatgt ctggtgttat gcatacccct atgcgtagcg tactttcact
     6601 ctggtgaact gatgatgagg ttcgttttct tctggccgtt ttttatgtcc attatgtgga
     6661 ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg
     6721 caccagctcc ccagttgaaa gataatccgt ctatctccat tatcattccc tgttttaatg
     6781 aggagaaaaa cgttgaggaa accatacacg ccgctttagc acagcgttat gagaacattg
     6841 aagttattgc cgtaaatgac ggttcaacag ataaaacccg tgccatcctg gatcgcatgg
     6901 ctgcacaaat tccccatttg cgggtcattc atctggcgca aaaccagggg aaagccattg
     6961 cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg
     7021 cgttattaga ccgcgatgcg gcggcatata ttgtggaacc gatgttgtac aacccgcgtg
     7081 tgggtgccgt aaccggtaat cctcgtattc gaacacgttc taccctggtg ggtaaaattc
     7141 aggttggcga gtattcctca attattggtt tgatcaagcg aacccagcgt atctatggaa
     7201 acgtatttac cgtttccggt gttattgccg catttcgtcg cagcgccctg gcagaagtgg
     7261 gttactggag tgacgatatg atcaccgaag atattgatat tagctggaag ctgcagttga
     7321 atcagtggac gattttttac gagccacggg cgctgtgctg gatattaatg cctgaaacgt
     7381 taaaagggct gtggaaacag cgcctgcgct gggctcaggg cggtgcagaa gtattcctca
     7441 aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat gtggccgctg ttttttgaat
     7501 actgcctgac gacaatatgg gccttcacct gcctggtcgg tttcattatt tacgcagtcc
     7561 aacttgccgg tgtaccgtta aatattgaat tgacacatat cgctgcgaca catactgccg
     7621 gaatattatt gtgtacgtta tgtttactgc aatttattgt cagcctgatg atcgagaatc
     7681 gctatgagca taatctgact tcatcgcttt tctggattat ttggttcccg gttattttct
     7741 ggatgctgag cctgacaacg acattggtat catttacacg agtcatgttg atgcctaaaa
     7801 agcaacgcgc ccgttgggta agtcccgatc gcgggattct gagaggttaa tatgaacaat
     7861 ttaattatta cgacccgaca atcaccagta cgtttactgg ttgattatgt tgccacaacc
     7921 atcttgtgga cattatttgc gttgttcata ttcttattcg ccatggatct gctgacgggt
     7981 tattactggc aaagcgaggc cagaagccga cttcagttct attttttgct ggcagtggcg
     8041 aatgccgtcg tgttaattgt ctgggcgctg tacaataagc tgcgttttca aaaacagcag
     8101 catcatgcag cctaccaata tacgccgcaa gaatatgcag agagcttagc aatacctgat
     8161 gagctctatc agcaactaca aaaaagccac aggatgagcg tacacttcac cagccagggg
     8221 caaataaaaa tggttgtttc agaaaaagcg ctagtccggg cataaacacc caaaacaaag
     8281 cccggttcgc ccgggctctg caccgataat acacttaact gtatgcatgc agcgtacgtt
     8341 ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg gacgatccca accatttcat
     8401 cttcttcgaa acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc
     8461 attgcgtgat atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca
     8521 ttttcatttg cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa
     8581 aggtacgtcc acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca
     8641 ggcagtactg cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa
     8701 aatagggagc aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcggctt
     8761 gcagaactgg acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg
     8821 cttttgctgc gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct
     8881 gcttactctc aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg
     8941 aagtatcgcg actgtcgcgg gccataccaa tggcttctac gccgcccatc tgcacaagcg
     9001 aggtgaccga ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt
     9061 ttgcctcacg acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt
     9121 gtttgtattc atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt
     9181 ggcttccttg taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac
     9241 cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa
     9301 gttgctaccg gaggagaaat ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc
     9361 agaacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc
     9421 cgctgctact taataattac aacagatctc gcattcattg caacaatata ttcacttata
     9481 tttaaccata aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat
     9541 gacagatatg aaaaaagctg tgtgctttca gcaggattcg catcaggcat ctgcgcacga
     9601 acgccggatg cggcgtgaac gccttatccg cctaccgaac ccgcaccgat tgtaggcata
     9661 agaagacgcg caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctataaac
     9721 attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg
     9781 caaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc
     9841 ttttttgtac agtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc
     9901 ccatatccag ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca
     9961 tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg
    10021 caatcacctt cccttccgga tcgctggcgt aatcgggaac atcatgctca tgctgcattc
    10081 ccagcggcgc accggtttgc ttatcacgac caaaaatcgt ctgctgttct ttcagcggag
    10141 ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga
    10201 ttgtccacgc tggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat
    10261 cattctggct atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct
    10321 ctttgccttt actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc
    10381 tgagcaaatc tggcgtatgt ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg
    10441 tgttggcgca aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt
    10501 tgggaaaacg cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct
    10561 catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc
    10621 cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc
    10681 cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg
    10741 gtttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca
    10801 ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa
    10861 gcgtacccgg cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg
    10921 ccagcgcacc aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat
    10981 cttcatactg cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc
    11041 cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta
    11101 ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt
    11161 ggcatcgact tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc
    11221 gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta
    11281 gcgatcttct tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc
    11341 gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac
    11401 atcggtataa agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa
    11461 tgctttttcc agacggtgga aaccggtgaa tttcggatcg gcggcttttt gctcgtaatc
    11521 atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcgat
    11581 gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc
    11641 tttaatagcg tcagtaaagg ctttcgtgtc ggtcaccagc tgcgtggttt ccgccatgac
    11701 atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc
    11761 atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca
    11821 ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa agccaggggc
    11881 gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt
    11941 ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg
    12001 ttcgcactgc ttatcggtca cggtcacctt gacctgcggc acattagcgg cgttagccat
    12061 aaaagcagaa gagaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat
    12121 ggtcatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat
    12181 ttgctacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagggcaa atgccaccag
    12241 cgccgggatg agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata
    12301 gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat
    12361 atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc
    12421 accagccgcc agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc
    12481 accaagattg aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc
    12541 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
    12601 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
    12661 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac
    12721 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
    12781 gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt cgccggtggt
    12841 ttcgttaatg aagataccca ggcccaggca caacgcagcg gcaagcaaca cgccaatcca
    12901 catcacacca atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac
    12961 aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc
    13021 cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg
    13081 attatcatta cggtaaagaa aaatacaagc ggaatgttta taaaatgatg ttggaatgta
    13141 aacaaagaga ggtaatgcca agggatataa acgattattc cagggtagaa agatgggatt
    13201 gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa acgccgtatc cagccgacaa
    13261 aactgtccag actcaatgca tcagagcttc catgtaaaac tgataagcgg gtgtatcaag
    13321 caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata ataacagctc
    13381 gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccgggaca gcagggggcg
    13441 gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg gcgaggcaca
    13501 ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta agtcccttag
    13561 ctttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt
    13621 ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat cccaatactg
    13681 ccgaagataa agcccggaat aatttcgtag agtcccagcc agccgaactg cttccagacg
    13741 ataaccgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac
    13801 cacatcaccg agaacagcac caccggacca aacgccgcgc caaagcctgc ccacgcgtag
    13861 ctcactaagc ccagcacgcg gttttccggg tttgccgcca gcgcaatcgc caccagcgcc
    13921 accaccagca ccatcacacg ccctacccac accagctctt tctggctggc atgtttacgc
    13981 agaaacgctt tgtataaatc ttcggtaatc gcactggagc acaccagcag ctggcagctt
    14041 aacgttgaca ttaccgccgc cagaattgcc gacagcagaa tcccggcaat ccacgggtta
    14101 aacagaattt gcgccagttc gataaacaca cgttcagcgt tctggtttac cgcacctgct
    14161 accgccgggt gctcattaaa gtaagcaatc ccaaagaagc cgacagccac cgcccctgcc
    14221 aggcagagga tcatccaggt catactgata cgacgcgcat ggacaatgct gtggtgagaa
    14281 tccgccgcca taaaacgcgc cagaatatga ggctgaccga agtaacccag cccccaaccc
    14341 atcaatgaga taatggcaac aaagttcagc cctttgagca tatccacgtt ttcgatgctc
    14401 ttttgtttga tcacttccag cgagtcacca aagccaccga cactgataat gacgataacc
    14461 ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt ccagctcacc
    14521 gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagcgcccgc ccacagagcc
    14581 gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac aatgcccgaa
    14641 gcgcaataaa tggtgaagaa cagcaaaata accagcgcag agataatgcg caaaatgcgg
    14701 cttttatctt caaagcgccc ggtgaaataa tccggcaatg ttaaggcgtt attgttgtat
    14761 tcggtatgca cacgcaaccg cccggccacc agcttccagt tgatccacgc gcctaatgtc
    14821 aggccaatgg cgatccagct ttcggaaatc ccggaaagaa aaaccgcgcc cggcaacccc
    14881 attaacagcc agccgctcat atcagacgca cccgccgata atgccgtcac gaatggccca
    14941 aggctacgac cgcccagaat atagtcgtcg aagtttttcg ttgatcgcca ggcgataaac
    15001 ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg tgtgctaata
    15061 gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg ttatatctgc
    15121 caccggaacg gggtaacaga gtttatgttt taccagggcg accgtatcct gccggaagcg
    15181 ctggttattc acaatcgatt taacacacca tttacattaa attttagtgc tcagcgacac
    15241 tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc aaaaatgtta
    15301 actgccgcag agaaaaagtc tgagttattt ttttaatccc tgtcatatcg atttctttta
    15361 ttaacatttc attcattttt aagcttgcta cgcatgtcac atttaacatg gttgcacaaa
    15421 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
    15481 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
    15541 taagtctgcc gcgacacgta tcgatcgcac accacactgg ttaattaagc aggcgatttt
    15601 ttcttatctc gaacaactgg aaaacagcga tactctgccg gagctacctg cgctgctttc
    15661 tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac accaaccttt
    15721 cctcgacttt gccgagcaaa tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc
    15781 ctatcgccgc ccggaaaccg aagcggtttc tatgctgttg gaacaagctc gcctgccgca
    15841 gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
    15901 aaaaaatgcc agtggtcgcg caggtatggt ccaggggtta ttgcaggagt tttcgctgtc
    15961 atcgcaggaa ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa
    16021 agccacccgc gacgcgttaa ttcgcgacaa aatcagcaac ggtaactggc agtcacacat
    16081 tggtcgtagc ccgtccctgt ttgttaatgc cgctacctgg gggctgctgt ttactggcaa
    16141 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
    16201 aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
    16261 gttcgtcact ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa
    16321 aggtttccgt tactcttacg atatgctggg cgaagccgcg ctgaccgccg cagatgcaca
    16381 ggcgtatatg gtttcctatc agcaggcgat tcacgccatc ggtaaagcgt ctaacggtcg
    16441 tggcatctat gaaggtccgg gcatttcaat caaactgtca gcgctgcatc cgcgctacag
    16501 ccgcgcccag tatgaccggg taatggaaga gctttaccca cgtctgaaat cactcaccct
    16561 gctggcgcgt cagtatgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga
    16621 gatctccctc gatctgctgg aaaaactctg tttcgagcct gagctggcag gctggaacgg
    16681 tatcggtttt gttattcagg cttatcaaaa acgctgccca ctggtgattg attacctgat
    16741 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
    16801 ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg
    16861 caaggtgtat accgacgttt catatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa
    16921 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact
    16981 ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
    17041 gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg
    17101 tatttatgct ccggtcggta cccatgaaac gctgttggcg tatctggtgc gtcgcctgct
    17161 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
    17221 tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
    17281 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
    17341 ctcggcaggg ctggatctcg ctaacgaaca ccggctggcc tcgctctctt ctgccctgct
    17401 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg
    17461 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga
    17521 agccacgccg cgtgaggtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
    17581 gtttgccact cctccggttg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
    17641 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
    17701 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg
    17761 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg
    17821 gaacttcccg ctggctattt tcaccgggca gattgctgcc gcactggcgg caggtaacag
    17881 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
    17941 gctggaagcg ggtgtaccgc caggcgtggt gcagttgctg ccaggtcagg gggaaaccgt
    18001 gggcgcgcaa ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga
    18061 agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat
    18121 tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
    18181 cgaacaggtc gtcgtggatg tactggcctc ggcgttcgac agtgcgggtc agcgttgttc
    18241 ggcgctgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg
    18301 cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
    18361 ggtgattgat agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa
    18421 aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag
    18481 cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga
    18541 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat
    18601 cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga
    18661 aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat
    18721 ggtgggcgca gtggtgggtg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc
    18781 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
    18841 ggcagtgacg ctcgcgcatc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc
    18901 attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc
    18961 gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc
    19021 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga
    19081 tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact
    19141 gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga
    19201 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
    19261 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc
    19321 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
    19381 tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac
    19441 gatcggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
    19501 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat
    19561 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag
    19621 tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt
    19681 tgacccaacc ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca
    19741 gttcatccat taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct
    19801 gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg
    19861 gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
    19921 gcacggcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
    19981 cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
    20041 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac
    20101 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
    20161 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt
    20221 tatgtgcaac tgttttgacc gtttagtcca ctttttacca gatattttag acaacacaat
    20281 gttaacacat taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat
    20341 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
    20401 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
    20461 atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa
    20521 aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
    20581 ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg
    20641 gccgtcacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca
    20701 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac
    20761 ctcgtgactg gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac
    20821 tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg
    20881 ggcacgggga aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt
    20941 ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg
    21001 ttctccgccc agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacgcccacg
    21061 gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt
    21121 ggttcttatg tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa
    21181 tgggaacact acaaagcggg cgcggatgaa gagacgttaa gctggctaac cgaacaaagt
    21241 cagaaagata cccgctccgg tactgatacc aacgtccgtc agatggccga tcccacttcg
    21301 gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta
    21361 gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg
    21421 tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatcta
    21481 cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta
    21541 ccttcgatcc gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct tatgccacgc
    21601 caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca
    21661 acattcaaac cgccgtgacc gcagcgcgag cggcagggat gctgatcatc tggtttcaaa
    21721 atggctggga tgaacagtat gtcgaagctg gcggccccgg ctcaccgaat tttcataaat
    21781 cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag
    21841 gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc
    21901 cgaagccgcg ctacagcggt ttcttcaata caccgctgga cagcattttg cgcagccgtg
    21961 gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac
    22021 gcgatggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg
    22081 ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca
    22141 gcgacgtcga aacgttctgc gacgcgcttt ctcccacatc ctttgctcgt atcgcttaag
    22201 gagtttaacg atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc
    22261 cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga
    22321 tcaacataat aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga
    22381 aattatccgc aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag
    22441 catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt
    22501 tccgggtgat aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct
    22561 ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct
    22621 cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg
    22681 gcagttactg gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg
    22741 accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga
    22801 tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcattac gcggtggtcg
    22861 gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa
    22921 ctgtgctgat cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc
    22981 aggttcgcga acgattactg tatagcggcg gcgcgcaggc atgggtggaa gcgcagccgt
    23041 tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg
    23101 cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca
    23161 aacgcgctga ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg
    23221 ccagtgatga tctgctggtg ccatcagcat gttccagtga gcttcatgcc gccctgcccg
    23281 atagccagaa aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa
    23341 cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc
    23401 tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ttgttcaccg
    23461 atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga
    23521 tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt
    23581 ttatccgcac ggcagaagga aaagaacgtc tgcgcccggc actttccagc ggcaatctgc
    23641 aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac
    23701 ggttaccgca actgtttcct cacggcgatg cccgcagttg gtttacctcc agcccacaac
    23761 ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct
    23821 gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg
    23881 acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg
    23941 atagcagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt
    24001 tgtaaggagc aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt
    24061 atgggcgcgg cagtcaatat catcaccacg gatggtccag ccgggcgcgc cgggttcacc
    24121 gccagcgcgg tctgcagcgt gaccgacacg ccgcctacat tactggtgtg cctgaatcgt
    24181 ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc
    24241 gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt
    24301 gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt
    24361 tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc
    24421 gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt
    24481 tatcacgcgc tgatgcgccc tgcttgttaa cctcctaaag gagacagctg atggcaatgt
    24541 tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgt
    24601 atgaacgatt gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt
    24661 ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa
    24721 tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc tccagttatc
    24781 tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg
    24841 gcattaaccc gaatatcagc attgcgctgg gcgggattat cgcctgtggg ctggtttata
    24901 ccgtcattgg cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc
    24961 cggtggtgac gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga
    25021 aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg
    25081 ggctggtggc ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggtttaa
    25141 ttgtcgcctg tctgctgtat ggcgtgatga ccaacctttt agggctggga aaagccgtcg
    25201 attttactct cgtcagccat gctgcctggt tcggtttgcc acatttttcc actcctgctt
    25261 ttaatagtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact
    25321 taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc
    25381 gggcattcgt cggtgatggg ctggcgacga tgctttctgg ttctgtcggt ggcagcggcg
    25441 taaccaccta cgccgagaat atcggtgtaa tggccgtgac aaaagtctac tcgacgctgg
    25501 tgtttgtcgc tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc
    25561 tgattcatac cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga
    25621 ttgccgtcgc aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta
    25681 atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct ctcacgctgg
    25741 gcggttttac gttgggaggg attggtacag caacctttgg cgcgatttta ctcaatgcgt
    25801 tgttaagcag aaagttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa
    25861 cagaaaaccg ccgggattac cggcggtgat gactttttga cgagcctaag aaaaaaatgc
    25921 attcgtcatt gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt
    25981 tttacctgct tcagatgcgc gctgcgggtc attcttgaaa ttacccccgc tgtgctgtcc
    26041 acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc
    26101 tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc atagaattaa
    26161 cgcggcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc
    26221 gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct
    26281 caaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt
    26341 cattttgcga caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga
    26401 aatttgcact ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa
    26461 tagagataac acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg
    26521 tacggacata ttgaaaccat gaagcattaa aaacacagaa ttaccgaaga tcgtccaaga
    26581 gcatatattt ttcaatctgt tacgcctcat tttgaccatg agaaaatcga tttcacaccg
    26641 ttctttatac gttctttttt gccccaaata tgccccaaac aaccacattt tgccccaata
    26701 gtgccccaaa gatttaaggg aatttaagta gatatttcag atgattaacg caagtttacg
    26761 atgtgaattt acgttaattt tctgggtagg gtatttaccg tcattcgttc atcaatattt
    26821 ctgcatattg acaaatacgc ctgagctatc tcgggagaaa tgcaatgaaa gcagcaaaag
    26881 gcgatgttaa tcaccctgct catctctgta ttaccgtcat tgtgacggca ctggtaacta
    26941 agaaagacct ctgcaaggga ggaatccctc gccacctctg atgtgtcagg tatcctcacc
    27001 ctccccaatc acattagccc cgcacttccg tacggggcat gttctttact ccacccccac
    27061 ttttttgttg tactcgcggt taaagcgatc cacggcaacc ttcatatccc gctctaccga
    27121 tttcaccatc gccgactgtt gcgccagact gagagtactg tcggcgtaaa tggcatcacg
    27181 ctgtttacgc aaatccttca gtctcttccg ggtatcctgc ataagcccgt tcatcgacaa
    27241 ttttccgttg ttctcgtcaa tgaacgccgt tctttctgca ccggtcagac tcttcagctc
    27301 tgcgtgatac tgcgcgacct ccgtcatccg gtcgtacatc ttctgctggt cagcatacgg
    27361 catcacctca cccgaaattt tccccaggaa aggcacctgc tgctccggta tatcaatacc
    27421 attcagcgat ttcaccgccg catccgtggt tttggaaatg aagcgccctg tccctccgga
    27481 gatatagtca atccagaatt tcagcgattc cggtgtgata tccaccgcgc cgggacggta
    27541 ctggctgcca cctgagaacg cattcagcca ggatgcaaac gccttgtacg cttctggcgt
    27601 tgaacgtctt cccagctggc tgtcaggttt tggtgtacca aacggcatgt tctcctggta
    27661 aatctgcgcc cccatgaagt tttcattcat ggcaaggttc gcaaacggac gcagaatggt
    27721 cggcgctgca tttttcagca atgccccgga cagtgtttcc gacgtttcac tgcccacagg
    27781 actgaatgcc ccaagcacac caccaacaac attaccggca gcacgtgaag cggtcaggtc
    27841 gcccgctgcc accccttcgg ctgtgtgacc aagcaggaag aaaacgttgt acccgtaagg
    27901 cagaggaata ctccagtact ctccggcctt gcccccgaac accgatttca taatgacgag
    27961 gttacgctct ttcacatgag acggcacctt gtcataccag ttaaccccgt catcatcctc
    28021 acccgccacg ctgcggttaa gcgagccaag cagataaccc gctcccacag cggcaagcgc
    28081 gattttctgc ggaacattca gattcttcca gcgaaggcgc tccagtaaag gtccctctcc
    28141 attaagatgt ccgagcgttc tcaccagatt tgccgttccc tgaatgctgg cgttggcgaa
    28201 catgtacagc gaattcatca gcgccccctg ctcaccacga cggttaaagt tcaccgtcat
    28261 atttttggca agagacgccg cctgctggcg tgacaaaccg gcatcacggg catgtttata
    28321 ggcagaaaga cgcagagcgt tttcaaccgc accgttggca tcctcgacaa ggttaaggaa
    28381 cgaattccat gcaccgatac tctggccttt ccatcccccc ttcgccagtg agacaaggcg
    28441 atccatttcc ttctgctggc cttcaaggtc acccatgtta aaccagccgg ttttccctcc
    28501 gtcctcaaca aactctttcc acaccttctg ccactgtgca ccttttcccg tgagggtttt
    28561 accacgcaga ctggcgtata cggctgacat ggcagaacgg ctgtctttgg ctacagccag
    28621 ggcggataag ttatccagcc ctttcagttt accgtcgctc cttccctgct ccgccttcag
    28681 gttcatcacc gccgtctgca cgtcacggat gaagttactg accaggaatt ccgggttata
    28741 cgacgtgttc accgttgcca ggaagcggtt aactttcccc agcgtacgaa caaaggcatt
    28801 gctggtttcc ggtcccatac tcttcatcgc acgcatcagg cgcggatcat ggagtttgat
    28861 gtagtacgtt ttgccgttct ttttggtggt gaagtaccgg tctgccatca ttgccatcgg
    28921 tacagggcgt tcgacaactt cgcgacgggt ttcaccggtt tccggatcaa cacgctctgc
    28981 aatcgcacgc atggtatcag gtctgtcatc agtgaagacc tgccagtaat ccttgtcagg
    29041 attatcctgc accagtttca ggaaggcgtt acccacttcg tttttgcgat ggcggatcag
    29101 cgattcgctc aggtcctgta tcgcctgagt ggacggagac tgtgcacggg atgcacgccc
    29161 catggcctgc ctgctttcac gtccgccgat ggtgaagccc ttacctgtac ggggcagtga
    29221 caccataccg tcaacatcct gacctttcag gggaacgtag taacggtagg cgttctgcca
    29281 ggcatccacc actccgctct cttccagtcc ggcctcacgg ataagctcac ggcgacgggc
    29341 cagcatatcg tcaacaatcc cagccagacg gtcatactgt gcctgtttgc cactgttacg
    29401 cacacgctgc atgatttccg ccgcttccgc gttggtcatc cccgaaccgc cgtccggcat
    29461 tttcgggttg attttcgcga tatgtgcgtt acgttccggc gcgtgacggg cgtagaggta
    29521 ctcatccaga tcggcctgcg caattttgta gtccgccagt aatttagcca gtggctgaac
    29581 gtagcgctcc ttcatcacgt tcaggtcgtt ttccgccttc ccgtggaaga gttcttccgc
    29641 catataagcg ttgttactat cgtctatttt tccgccagtt ttacggatat tctcctgaac
    29701 agctttcagc acccggaatt tatcctgcat ctggcgcaca aaacgcgata taattatctc
    29761 ttccggtgtc agactgctgg tacgggagta atacggcccc ttgcgaatat cttcaggata
    29821 gagtatttta tctacagagc cgcgataaga acgctcctct ttgggcaatt ggagcccact
    29881 gtactgaggg ttatcgcggc ttaatttttc cttccggtaa agcgttaatc cagccatttc
    29941 catgcttttc agctttttcc cgttttccgt accatataca gaagccactt tgttaatttc
    30001 cagacgttta cctgtcgcct tcatgtgcac tgccgacacc accggatcgc catttttatc
    30061 cacggcatca agcagcatca caactgcatt tctttctgtc gcggaacggt aaattgcatc
    30121 cggatcatgc atcagttccg ggagtctctc gataacatcc attggcacca catgtttcac
    30181 gccattggtt gccttccgta cagtgtcgcg ggagataacc agcggcaaat ccggtgcgcc
    30241 aaggtggcgc aataccggcg gcgtacgccc aatgtttgca gtcattgcac cagaacgtaa
    30301 cgacttcatc attctggcaa ggtcatcacg ataacgttca ccctcacctt ccggcacttt
    30361 gaacggatca ggcttaccac tacgggagta ctgagacgat gcgcccacgc catcctcacg
    30421 cggcgtgtaa ccttcccgca cacgctggcc taacgtacgg atggtctcgc gaacaagtct
    30481 gatatcgttc agttccgtcg gcttcagtaa ccccgtacga cgcagtaccc ctttgaccag
    30541 agcaacaaca cgctcccatg ccgccacgaa tttattcggc tgtttctccg ccatatgtgc
    30601 cagaaattca cccgcctgta cttccggaga ttccttacca taggacgcat caaccttacg
    30661 ccatgcttca cggatggtgg cgttatcact gtcgcgggtt ttcagcacgg ttttgataat
    30721 cgtctgatat tccgctggcg tgacaacatg ctccatggca tggtggataa tctcgtgacg
    30781 caacttctcg cgtacggtcc gcccgtcagg gatgttatcc gccaccagga caatttctcg
    30841 tttatccgga cgatagaatg cgtgcaccct gccgtaaccg tcgaacgatt cacccgccag
    30901 cgcttcagcc tctttctgtg acttcaccac acggaccttc aggtcactgt ccttaatgcc
    30961 gctcatcacg ccacgggcaa cagcttcaac ctgcgagacc ggactgcctt tggcttccgc
    31021 actgcggtta acatccgaaa tcagattgcc ttcaggtgtg cgggtcacgc ccttacggga
    31081 ataaaacgca actcccttgt ccgtctcacg ggttttcagt gttcggaaca agtgatcgaa
    31141 tgcctcacga ataccgccat ccagttctgc attcgtcgga taagcgtagg tgtccggctg
    31201 tccatggtca tccgccttac ggatattgac cagataatca ttctccacgc cagccatacg
    31261 cgctttatcc tgaacataac gctcaaaggc tcgcgccgcc atttcaacat ccgttgacca
    31321 gtacggtttt gaacgaacgc catccaggcg ttcagaacga cgcaacatgt cactgctgtt
    31381 aatggctttc atcacgcctt taaacgcgtc gtaaacctcc tgacgtaccg gatattccgc
    31441 atcaacatac ctgccgtctt taaatacgcg cccggcacgc tgtgcttccg tcatatagtc
    31501 gccacctgac gtgatcttcc cgtcagtgga aacatcataa cgaccaaaat aattatccag
    31561 tgaatggaac cattcgtggg ccagcgcccc cggtccgtta ccttttgtca ggttgattgc
    31621 cacctcaccc ggctcatagt gtgccgccgc cttaccctta ccacgggcac caaaagcaag
    31681 cccaagacga ccgttcagag aaagcgcttt tgtcggcacg ttcagcacgt cagccaggtc
    31741 atgcagcgag tcataagccc ggttcaaatc agcctgacga cgcggacttt ccacataatt
    31801 accaaactgc acaccacgaa aaccaaacgc atcactgaac tgctccggcg aaacatcccc
    31861 cttgcggcgt tctggtccgg tacgatcgcg gttggtggcg ttacgctgct cctcacgcga
    31921 gacttcacgc agcgccttca cctgattaac cagctcatca cgatgtgtgc gcacataatc
    31981 atgtgcatca cgggcagact caaaaccgcc cttcagtttc tgcctgtttt tgccataacc
    32041 gataaaaatg tcgccactgg ccttatgccg gtacacgtca aaggttattc tgtcatcagc
    32101 agaggttgct ggcgtttccc tttctctgga gccttgttca tcgaaatatg ctttcgcctt
    32161 tttcagtaac tcatcccggc tttccgagaa aaagaggtcg cgccccttgc tatcacgaag
    32221 cgaatacatc atttgcggcg gatcgtaacg ttttcccccg acaaacgaat aaacaccgga
    32281 cctgacacga tagccggatg ctttatcaat ctgcaatgca gaaagcgtac ggagcagttt
    32341 ccaggtatcc gacatgctcc gtaaccccgg cttactgtcc atcatatcag ccagtttcgc
    32401 tgccggaatt ttcccctcca gcataccggc agtagcttcg cgaccgcttt taacaatgct
    32461 gatccatttc tgcatgttac ggcttgttct ggtgggaatt tcactgcgat acaacgccat
    32521 cattgccagc gtgtcactat ccacaccttc tttttccaga cgagcataat ccggctttgg
    32581 aaacagtttg ctcagtggtt gtgtcgcgta ctccccatcc tccagcgttt tacccatagc
    32641 ttcagcaagc tgcgcataac ggtgttttgc cgcgccctta atctcctcgc caaagtcttc
    32701 aattttcgca ccacgcgcct gctggtgagt gctcggttta tctaccggca cctcagtatc
    32761 cggaacatcg gttatggtct ggcgatcccc ggtctgtctg cgtgcttctg cctgctgtgc
    32821 cagttccgga agggagtcac gaaccatacg agggagtgat tcaggctgtg gcatatcaac
    32881 cccacgctga ctttcaggtg caggaagtcc ttcacgcact ggtgctgtgg cgttctgcgc
    32941 atccggtgac ggcagaccac gacgaaccgg ttcgccctcc agaatttccc cctggcgacc
    33001 agcggcattt tccggtgcct gcgcacgtcc tttctggaaa ctctggccct tcacctcacc
    33061 ggttgtggta aaacgaccac cacgtccggc ctgattttca tccggcgtac gcgccacttc
    33121 tcccggtaac ggatatccct gtccgggatg aatatcgccg ggggcgggca gacgcggacg
    33181 ctcagttaac tcctgtgctg tcggaccggc atcaccttca gccatctgtg aacgtacaag
    33241 ctcctccgcc gtcggcgc
//
LOCUS       DAZFVO020000040        32438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFVO020000040 DAZFVO020000000
VERSION     DAZFVO020000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 32438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.040"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(71..1831)
                     /gene="ycaO"
                     /locus_tag="ACHKNB_RS08810"
                     /old_locus_tag="pgaptmp_001762"
     CDS             complement(71..1831)
                     /gene="ycaO"
                     /locus_tag="ACHKNB_RS08810"
                     /old_locus_tag="pgaptmp_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HGU4195292.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1961..2653
                     /locus_tag="ACHKNB_RS08815"
                     /old_locus_tag="pgaptmp_001763"
     CDS             1961..2653
                     /locus_tag="ACHKNB_RS08815"
                     /old_locus_tag="pgaptmp_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGU4195293.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2852..3940
                     /gene="serC"
                     /locus_tag="ACHKNB_RS08820"
                     /old_locus_tag="pgaptmp_001764"
     CDS             2852..3940
                     /gene="serC"
                     /locus_tag="ACHKNB_RS08820"
                     /old_locus_tag="pgaptmp_001764"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HGU4195294.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIECPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4011..5294
                     /gene="aroA"
                     /locus_tag="ACHKNB_RS08825"
                     /old_locus_tag="pgaptmp_001765"
     CDS             4011..5294
                     /gene="aroA"
                     /locus_tag="ACHKNB_RS08825"
                     /old_locus_tag="pgaptmp_001765"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGU4195295.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5463..6227
                     /gene="ycaL"
                     /locus_tag="ACHKNB_RS08830"
                     /old_locus_tag="pgaptmp_001766"
     CDS             5463..6227
                     /gene="ycaL"
                     /locus_tag="ACHKNB_RS08830"
                     /old_locus_tag="pgaptmp_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HGU4195296.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADYFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6400..7083
                     /gene="cmk"
                     /locus_tag="ACHKNB_RS08835"
                     /old_locus_tag="pgaptmp_001767"
     CDS             6400..7083
                     /gene="cmk"
                     /locus_tag="ACHKNB_RS08835"
                     /old_locus_tag="pgaptmp_001767"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGU4195297.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7194..8867
                     /gene="rpsA"
                     /locus_tag="ACHKNB_RS08840"
                     /old_locus_tag="pgaptmp_001768"
     CDS             7194..8867
                     /gene="rpsA"
                     /locus_tag="ACHKNB_RS08840"
                     /old_locus_tag="pgaptmp_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGU4195298.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9027..9311
                     /gene="ihfB"
                     /locus_tag="ACHKNB_RS08845"
                     /old_locus_tag="pgaptmp_001769"
     CDS             9027..9311
                     /gene="ihfB"
                     /locus_tag="ACHKNB_RS08845"
                     /old_locus_tag="pgaptmp_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HGU4195299.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9518..11782
                     /locus_tag="ACHKNB_RS08850"
                     /old_locus_tag="pgaptmp_001770"
     CDS             9518..11782
                     /locus_tag="ACHKNB_RS08850"
                     /old_locus_tag="pgaptmp_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HGU4195300.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11819..13567
                     /gene="msbA"
                     /locus_tag="ACHKNB_RS08855"
                     /old_locus_tag="pgaptmp_001771"
     CDS             11819..13567
                     /gene="msbA"
                     /locus_tag="ACHKNB_RS08855"
                     /old_locus_tag="pgaptmp_001771"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HGU4195301.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13564..14550
                     /gene="lpxK"
                     /locus_tag="ACHKNB_RS08860"
                     /old_locus_tag="pgaptmp_001772"
     CDS             13564..14550
                     /gene="lpxK"
                     /locus_tag="ACHKNB_RS08860"
                     /old_locus_tag="pgaptmp_001772"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HGU4195302.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14587..15819
                     /gene="ycaQ"
                     /locus_tag="ACHKNB_RS08865"
                     /old_locus_tag="pgaptmp_001773"
     CDS             14587..15819
                     /gene="ycaQ"
                     /locus_tag="ACHKNB_RS08865"
                     /old_locus_tag="pgaptmp_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HGU4195303.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15871..16053
                     /gene="ycaR"
                     /locus_tag="ACHKNB_RS08870"
                     /old_locus_tag="pgaptmp_001774"
     CDS             15871..16053
                     /gene="ycaR"
                     /locus_tag="ACHKNB_RS08870"
                     /old_locus_tag="pgaptmp_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HGU4195304.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16050..16796
                     /gene="kdsB"
                     /locus_tag="ACHKNB_RS08875"
                     /old_locus_tag="pgaptmp_001775"
     CDS             16050..16796
                     /gene="kdsB"
                     /locus_tag="ACHKNB_RS08875"
                     /old_locus_tag="pgaptmp_001775"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HGU4195305.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16950..17843
                     /locus_tag="ACHKNB_RS08880"
                     /old_locus_tag="pgaptmp_001776"
     CDS             16950..17843
                     /locus_tag="ACHKNB_RS08880"
                     /old_locus_tag="pgaptmp_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HGU4195306.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="ACHKNB_RS08885"
                     /old_locus_tag="pgaptmp_001777"
     CDS             complement(17820..18599)
                     /gene="elyC"
                     /locus_tag="ACHKNB_RS08885"
                     /old_locus_tag="pgaptmp_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HGU4195307.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18735..19520
                     /gene="cmoM"
                     /locus_tag="ACHKNB_RS08890"
                     /old_locus_tag="pgaptmp_001778"
     CDS             18735..19520
                     /gene="cmoM"
                     /locus_tag="ACHKNB_RS08890"
                     /old_locus_tag="pgaptmp_001778"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HGU4195308.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19517..20839
                     /gene="mukF"
                     /locus_tag="ACHKNB_RS08895"
                     /old_locus_tag="pgaptmp_001779"
     CDS             19517..20839
                     /gene="mukF"
                     /locus_tag="ACHKNB_RS08895"
                     /old_locus_tag="pgaptmp_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HGU4195309.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20820..21524
                     /gene="mukE"
                     /locus_tag="ACHKNB_RS08900"
                     /old_locus_tag="pgaptmp_001780"
     CDS             20820..21524
                     /gene="mukE"
                     /locus_tag="ACHKNB_RS08900"
                     /old_locus_tag="pgaptmp_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HGU4195310.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21524..25984
                     /gene="mukB"
                     /locus_tag="ACHKNB_RS08905"
                     /old_locus_tag="pgaptmp_001781"
     CDS             21524..25984
                     /gene="mukB"
                     /locus_tag="ACHKNB_RS08905"
                     /old_locus_tag="pgaptmp_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HGU4195311.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26245..28092
                     /gene="ldtD"
                     /locus_tag="ACHKNB_RS08910"
                     /old_locus_tag="pgaptmp_001782"
     CDS             26245..28092
                     /gene="ldtD"
                     /locus_tag="ACHKNB_RS08910"
                     /old_locus_tag="pgaptmp_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4195312.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28273..28821
                     /locus_tag="ACHKNB_RS08915"
                     /old_locus_tag="pgaptmp_001783"
     CDS             28273..28821
                     /locus_tag="ACHKNB_RS08915"
                     /old_locus_tag="pgaptmp_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HGU4195313.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28848..29495
                     /gene="gloC"
                     /locus_tag="ACHKNB_RS08920"
                     /old_locus_tag="pgaptmp_001784"
     CDS             28848..29495
                     /gene="gloC"
                     /locus_tag="ACHKNB_RS08920"
                     /old_locus_tag="pgaptmp_001784"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HGU4195314.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="ACHKNB_RS08925"
                     /old_locus_tag="pgaptmp_001785"
     CDS             complement(29717..30907)
                     /gene="aspC"
                     /locus_tag="ACHKNB_RS08925"
                     /old_locus_tag="pgaptmp_001785"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HGU4195315.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31092..32180)
                     /gene="ompF"
                     /locus_tag="ACHKNB_RS08930"
                     /old_locus_tag="pgaptmp_001786"
     CDS             complement(31092..32180)
                     /gene="ompF"
                     /locus_tag="ACHKNB_RS08930"
                     /old_locus_tag="pgaptmp_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HGU4195316.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7910 a         7882 c         8716 g         7930 t
ORIGIN      
        1 gcattaaaaa aacgccccgt aatataactc agactaatca ttaagcctac attgcgtagg
       61 ctatttgatt ttattttgcc cagaatgctg ctttggcgcg ctgcagcttt tcgtaggcct
      121 tcaacaacga ctgatgtgca gcaaacgcgt gcagatcgct atctaccggt tgcaggccgt
      181 aaaacgccgc ttcgccgctc attgccgcac tggcggcttc tacggcatct gcgccgtaca
      241 tgcgaacaaa cgcattcaga tattgcagcg gctggcgatc ttcttcctgt gccagtaata
      301 acaacgtttg caggcagcga taatagttgg cgcgttccgg actaaatact gatgagttaa
      361 actccatcgt ccattcggtc cagaccagag cctgttccag atcgccacca gccagcgcca
      421 gcatggcttt taattcaccg atacgcaggg tgtaccaacc gttatccgac ccggtcgcca
      481 gacccaacag ctcacgcacg cgggtaaagt catcaaaacc ttcttcatcc agttgctcga
      541 tgaggttcag gtaatcttct ttttcccact cgctgcctgg tagcgaaaga atcgtttcac
      601 gtaaatggct gcccatactg ttattcgcga gccacagatc ttcagccgga taaatatcgg
      661 acatgccagg cacgataatg cggcaagcgt agacacccag atgctcgtaa tcggcaatat
      721 aaacttcttt atcttccttt ttgaagatag ccatcagcgt ggcgaactct tcttccgtgg
      781 tgccggagaa attccagtcc acaaacggat aatcggcatc ctgcttgaac aggtcccagg
      841 agattaaacc gctggaatcg ataaagtgcg tttcgaggtt ggtatgttca gcgacttctt
      901 catcatcgaa ggttggcgga gtaaacacat ccaaatcttt caggccacga ccttgcagca
      961 gctcggtcac ggtacgttcc agtgctacgc caaaatcagg atgcgcaccg aaagaggcaa
     1021 agcaggtacc gttagcagga ttgaacagta ccacgcaaat caccggatac tggccgccaa
     1081 gcgaaccgtc ataagcgaag attgggaaac cctccgcttc cagcgtttcg atggcttcaa
     1141 ctactgctgg gtaacgcgcc agcacgtctg ccgggatctc tggcaggctg atgctttcag
     1201 caataatgcg gtttttcacg tagcgttcga aaacttcaga caatccctga acgcgtgctt
     1261 cgttgcgggt attacctgcg gacataccgt tggagacgta caggttacca atgatattca
     1321 tcggaatata aacggtctga ttgtcggact gacgcgtaaa cggcaggccg caaataccac
     1381 gatcttcgtt accggattgt aggtcaatca gcatgctgcc ggtcagttca ttctccggat
     1441 cataaaacgc gcgcagacgg tcatcgagta gtccttctgg cacatcgtca ttttcggtca
     1501 gtgggaacca tttttcgttg ggataatgca cgaacggacc gttggcgatg gtttcgccca
     1561 gccagaaatc agcaaaaaag tagttggttg agagacgctc gaaatattca ccaagtgcag
     1621 aagccagcgc cgcttttttg gttgcgcctt taccgttggt aaaacacagt gcgcactctt
     1681 tgtcgcgaat atgtacagac cagacgttag gcacaggatt cagccaggag gcctcttcaa
     1741 tctgaaagcc gaggtctgaa agtttttgct ggaagcgagc gatggaatct tccagagcgg
     1801 catctttgcc ggggataaat gtttgcgtca tgaaaatcac tttagtcgta cggaaagcgc
     1861 gcaataatac gggttttatc tcaaaggcgc tatcaccgcc gccatgccga tgaactgttg
     1921 actatgcttt tagcggataa accacgataa gagcataaaa atgaaggcgt tcgatctcca
     1981 ccgtatggca tttgataaag tgccttttga tttccttggc gaagttgcac tacgtagtct
     2041 ttataccttt gtactggttt ttctgttcct caaaatgacc ggaagacgcg gtgtgcgaca
     2101 gatgtcgctg tttgaagtgt taatcattct cacgctgggt tcagcggcgg gagatgtggc
     2161 gttttatgat gatgtgccga tggtgccggt acttatcgtc tttatcactc tggcgttgtt
     2221 ataccgcctg gtaatgtggt tgatggcgca cagtgaaaaa ctggaagatt tactggaagg
     2281 taagccggtt gttattattg aagatggcga gctggcctgg tcgaaactca ataactccaa
     2341 catgacggaa tttgagttct ttatggagct acgattgcgt ggcgtggagc agctggggca
     2401 ggtacgtctg gcgattctcg aaaccaacgg gcaaatcagt gtctatttct ttgaagatga
     2461 caaggtgaaa ccgggtttac ttattttacc cagtgattgt acgcagcgtt acaaagtggt
     2521 gccggagtcg gcggactatg cctgtattcg ttgtagtgaa atcattcata tgaaagcggg
     2581 ggaaaaacaa ttatgtccgc gctgtgcaaa tccagaatgg acgaaggcaa gtcgggcaaa
     2641 acgggtgacc tgacagtaaa aacatcggct ttttgctaat aatccgagag attcttttgt
     2701 gtgatgcaag ccacattttt gccctcaacg gttttactca ttgcgatgtg tgtcactgaa
     2761 tgataaaacc gatagccaca ggaataatgt attacctgtg gtcgcaatcg attgaccgcg
     2821 ggttaatagc aacgcaacgt ggtgagggga aatggctcaa atcttcaatt ttagttctgg
     2881 tccggcaatg ctaccggcag aggtgcttaa acaggctcaa caggaactgc gcgactggaa
     2941 cggtcttggt acgtcggtga tggaagtgag tcaccgtggc aaagagttca ttcaggttgc
     3001 agaggaagcc gagaaggatt ttcgcgatct tcttaatgtc ccctccaact acaaggtatt
     3061 attctgccat ggcggtggtc gcggtcagtt tgctgcggta ccgctgaata ttctcggtga
     3121 taaaaccacc gcagattatg ttgatgccgg ttactgggcg gcaagtgcca ttaaagaagc
     3181 gaaaaaatac tgcacgccta atgtctttga cgccaaagtg actgttgatg gtctgcgcgc
     3241 ggttaagcca atgcgtgaat ggcaactctc tgataatgct gcttatatgc attactgccc
     3301 gaatgaaacc atcgacggta tcgccatcga cgaaacgcca gacttcggca aagatgtggt
     3361 ggtcgccgcc gacttctctt caaccattct ttcccgtccg attgacgtca gccgttatgg
     3421 cgtgatttac gctggcgcgc agaaaaatat cggcccggct ggcctgacaa tcgtcatcgt
     3481 tcgtgaagat ttgctgggca aagcgaatat cgagtgtccg tcgattctgg attattccat
     3541 cctcaacgat aacggctcca tgtttaacac gccgccgaca tttgcctggt atctatctgg
     3601 tctggtcttt aaatggctga aagcgaacgg cggtgtagct gaaatggata aaatcaatca
     3661 gcaaaaagca gaactgctat atggggtgat tgataacagc gatttctacc gcaatgacgt
     3721 ggcgaaagct aaccgttcgc ggatgaacgt gccgttccag ttggcggaca gtgcgcttga
     3781 caaattgttc cttgaagagt cttttgctgc tggccttcat gcactgaaag gtcaccgtgt
     3841 ggtcggcgga atgcgcgctt ctatttataa cgccatgccg ctggaaggcg ttaaagcgct
     3901 gacagacttc atggttgagt tcgaacgccg tcacggttaa tgccgaaatt ttgcttaatc
     3961 cccacagcca gcctgtgggg tttttatttc tgttgtagag agttgagttc atggaatccc
     4021 tgacgttaca acccatcgct cgtgtcgatg gcactattaa tctgcccggt tccaagagcg
     4081 tttctaaccg cgctttattg ctggcggcat tagcacacgg caaaacagta ttaaccaatc
     4141 tgctggatag cgatgacgtg cgccatatgc tgaatgcatt aacagcgtta ggggtaagct
     4201 atacgctttc agccgatcgt acgcgttgcg aaattatcgg taacggcggt ccattacacg
     4261 cagaaggtgc cctggagtta ttcctcggta acgccggaac ggcaatgcgt ccgctggcgg
     4321 cagctctttg tctgggtagc aatgatattg tgctgaccgg tgagccgcgt atgaaagaac
     4381 gcccgattgg tcatctggtg gatgcgctgc gcctgggcgg ggcgaagatc acttacctgg
     4441 aacaagaaaa ttatccgccg ttgcgtttac agggcggctt tactggcggc aacgttgacg
     4501 ttgatggctc cgtttccagc caattcctca ccgcactgtt aatgactgcg cctcttgcgc
     4561 cggaagatac ggtgattcgt attaaaggcg atctggtttc taaaccttat atcgacatca
     4621 cactcaatct gatgaagacg tttggtgttg aaattgaaaa tcagcactat caacaatttg
     4681 tcgtaaaagg cgggcagtct tatcagtctc cgggtactta tttggtcgaa ggcgatgcat
     4741 cttcggcttc ttactttctg gcagcagcag caatcaaagg cggcactgta aaagtgaccg
     4801 gtattggacg taacagtatg cagggtgata ttcgctttgc tgatgtgctg gaaaaaatgg
     4861 gcgcgaccat ttgctggggc gatgattata tttcctgcac gcgtggtgaa ctgaacgcta
     4921 ttgatatgga tatgaaccat attcccgatg cggcgatgac cattgccacg gcggcgttat
     4981 ttgcaaaagg caccaccacg ctgcgcaata tctataactg gcgtgttaaa gaaaccgatc
     5041 gcctgtttgc gatggcaaca gaactgcgta aagtcggtgc ggaagtagaa gaggggcacg
     5101 attacattcg tatcactcca ccggaaaaac tgaactttgc cgagatcgcg acatacaatg
     5161 atcaccggat ggcgatgtgt ttctcgctgg tggcgttgtc agatacacca gtgacgattc
     5221 ttgatcccaa atgtacggcc aaaacatttc cggattattt cgagcagttg gcgcggatta
     5281 gccaggcagc ctgaatgaac aacgggcaat aaatagccaa atctttcttt atcaaaacgt
     5341 cggcacattg tcggcgtttt ttttcggacc ttgtgagtca ttttgattaa tggtagcgtc
     5401 gcttgtcaat gtaagttgtt gatacataat atttatatat gattaatcaa cggatgattc
     5461 acatgaagaa tactaaatta ctgctggcga ttgcgacctc tgcagcatta ctgacagggt
     5521 gtcaaaatac ccacggtatt gataccaata tggctatcag ctccggttta aatgcctata
     5581 aagcagcaac attaagcgat gccgatgcaa aagcgattgc caatcagggc tgtgccgaaa
     5641 tggacagcgg caatcaagtc gcaagtaaat ccagcaagta cggtaaacgt ctggcaaaaa
     5701 tcgccaaagc attgggtaac aatattaatg gcacgccggt caactataag gtttatatga
     5761 ccagcgacgt caacgcatgg gcgatggcga acggctgtgt ccgtgtctac agtggcctga
     5821 tggacatgat gaatgacaac gaaattgaag gtgttctggg ccatgaactg ggccacgtcg
     5881 cgttgggtca ctcgctggct gaaatgaaag cttcttatgc gatcgttgcc gcacgcgatg
     5941 ccatttcagc taccagcgga gtggcttccc agctttcccg ctcacaattg ggtgatatcg
     6001 cagaaggcgc tattaatgct aaatactccc gtgataaaga gtccgaagca gattatttct
     6061 cctttgatct gttgaagaaa cgtggcatca gcacccaggg gctggttggc agctttgaaa
     6121 aactggctag tctggatggc ggtcgcaccc agtccatgtt tgactctcac ccaccatcaa
     6181 cagagcgtgc gcaacacatc cgtgatcgta tcgcctctgg taagtaaatc attgtcatct
     6241 ttcgggctgg tcttctgcca gcccgctata attgcgcaat aaatccccat ctgaatacag
     6301 acaaaactgg tttttgcaca caacgttaac gatttgtggc gtcggcgcgt ataatgcgcg
     6361 cggttatgtt aacggtacgc ctgttttaag gagataaaga tgacggcaat tgccccggtt
     6421 attaccattg atggcccaag cggtgcaggg aaaggcacct tgtgtaaggc tatggcggaa
     6481 gcattgcaat ggcatctgct ggattcgggt gcaatttatc gcgtactggc attggcggca
     6541 ttacatcacc atgttgatgt tgcgtcagaa gatgcgctgg taccgctggc atcccatctg
     6601 gatgtacgtt ttgtgtcaac caatggcaat ctggaagtga tcctcgaagg ggaagatgtc
     6661 agcggcgaaa ttcgtactca ggaagtggcg aatgcagctt cacaagtcgc ggcattccca
     6721 cgcgttcgtg aagcattatt gcgtcgccaa cgcgcgtttc gcgaattacc aggcctgatt
     6781 gccgatggcc gcgacatggg aacggtggta ttccctgatg cgccagtgaa aattttcctt
     6841 gacgcctcct cggaagaacg tgcgcatcgc cgcatgctac agttgcagga gaagggcttt
     6901 agtgttaact ttgagcgcct tttggccgag atcaaagaac gcgacgaccg cgatcgtaac
     6961 cgagcggtag cgccactggt tccggcagcc gatgctttag tgttggattc caccacctta
     7021 agcattgagc aagtgattga aaaagcgcta caatacgcgc gccagaaatt ggctctcgca
     7081 taagcgaccg aatttgcagt acccccgttg caatggaatg acagcgggta tgttaaacaa
     7141 ccccatccgg catggagcca ggtggacgtt aaatataaac ctgaagatta aacatgactg
     7201 aatcttttgc tcaactcttt gaagagtcct taaaagaaat cgaaacccgc ccgggttcta
     7261 tcgttcgtgg cgttgttgtt gctatcgaca aagacgtagt actggttgac gctggtctga
     7321 aatctgagtc cgccatcccg gctgagcagt tcaaaaacgc ccagggcgag ctggaaatcc
     7381 aggtaggtga cgaagttgac gttgctctgg acgcagtaga agacggcttc ggtgaaactc
     7441 tgctgtcccg tgagaaagct aaacgtcacg aagcctggat cacgctggaa aaagcttacg
     7501 aagatgctga aactgttacc ggtgttatca acggcaaagt taagggcggc ttcactgttg
     7561 agctgaacgg tattcgtgcg ttcctgccag gttctctggt agacgttcgt ccggtgcgtg
     7621 acactctgca cctggaaggc aaagagcttg aatttaaagt aatcaagctg gatcagaagc
     7681 gcaacaacgt tgttgtttct cgtcgtgccg ttatcgaatc cgaaaacagc gcagagcgcg
     7741 atcagctgct ggaaaacctg caggaaggca tggaagttaa aggtatcgtt aagaacctca
     7801 ctgactacgg tgcattcgtt gatctgggcg gcgttgacgg cctgctgcac atcactgaca
     7861 tggcctggaa acgcgttaag catccgagcg aaatcgtcaa cgtgggcgac gaaatcactg
     7921 ttaaagtgct gaagttcgac cgcgaacgta cccgtgtatc cctgggcctg aaacagttgg
     7981 gcgaagatcc gtgggtagct atcgctaaac gttatccgga aggtaccaaa ctgactggtc
     8041 gcgtgaccaa cctgaccgac tacggctgct tcgttgaaat cgaagaaggc gttgaaggcc
     8101 tggtacacgt ttccgaaatg gattggacca acaaaaacat ccacccgtcc aaagttgtta
     8161 acgttggcga tgtagtggaa gttatggttc tggatatcga cgaagaacgt cgtcgtatct
     8221 ccctgggtct gaaacagtgc aaagctaacc cgtggcagca gttcgcggaa acccacaaca
     8281 agggcgaccg tgttgaaggt aaaatcaagt ctatcactga cttcggtatc ttcatcggcc
     8341 tggacggcgg catcgacggc ctggttcacc tgtctgacat ctcctggaac gttgcaggcg
     8401 aagaagcagt tcgtgaatac aaaaaaggcg acgaaatcgc tgcagttgtt ctgcaggttg
     8461 acgcagaacg tgaacgtatc tccctgggcg ttaaacagct cgcagaagat ccgttcaaca
     8521 actgggttgc tctgaacaag aaaggcgcta tcgtaaccgg taaagtaact gcagttgacg
     8581 ctaaaggcgc aaccgtagaa ctggctgacg gcgttgaagg ttacctgcgt gcttctgaag
     8641 catcccgtga ccgcgttgaa gacgctaccc tggttctgag cgttggcgac gaagttgaag
     8701 ctaaattcac cggcgttgat cgtaaaaacc gcgcaatcag cctgtctgtt cgtgcgaaag
     8761 acgaagctga cgagaaagat gcaatcgcaa ctgttaacaa acaggaagat gcaaacttct
     8821 ccaacaacgc aatggctgaa gctttcaaag cagctaaagg cgagtaattc tctgactctt
     8881 cgggattttt attccgaagt ttgttgagtt tacttgacag attgcaggtt tcgtcctgta
     8941 atcaagcact aagggcggct acggccgccc ttaatcaatg cagcaacagc agccgcttaa
     9001 tttgccttta aggaaccgga ggaatcatga ccaagtcaga attgatagaa agacttgcca
     9061 cccagcaatc gcacattccc gccaagacgg ttgaagatgc agtaaaagag atgctggagc
     9121 atatggcctc gactcttgcg cagggcgagc gtattgaaat ccgcggtttc ggcagtttct
     9181 ctttgcacta ccgcgcacca cgtaccggac gtaatccgaa gactggcgat aaagtagaac
     9241 tggaaggaaa atacgttcct cactttaaac ctggtaaaga actgcgcgat cgcgccaata
     9301 tttacggtta agtttttact caaacttgaa cgcgagaaaa gcacctgtcg ggtgcttttt
     9361 tcatttctct aatcaggaac tggaagctgc ctcgcagagt tttgagcagt tttcaccctt
     9421 tcgttaaatt cttctgaatg tgcctcgggg aacgcaaaat tcccaccaga caaccgctca
     9481 acaaagttgc acactttcca taaacaggga ggggtgcatg aaaataacga cagtcggcgt
     9541 atgcataatt tgcggaattt ttccgttgct gattttgccc caattgcctg ggacattaac
     9601 ccttgcgttt ctgactctct tcgcctgcgt actggcattt atccctgtta aaaccgtccg
     9661 ttatatcgcg ctgacgttgc tgtttttcgt ttggggcata ttatcagcaa agcaaatttt
     9721 gtgggcagga gaaaccttaa ctggcgcgac gcaggatgca attgttgaga tcactgcaac
     9781 tgacggcatg accactcatt acggtcaaat tactcatcta caaggtcgac gtatattccc
     9841 tgcgccaggc cttgtgctgt atggcgaata tcttccgcaa gcggtttgtg ctggacaaca
     9901 atggtcaatg aaactcaaag ttcgtgcggt tcatggccaa cttaatgatg gcggctttga
     9961 tagccagcgt tatgccattg cccagcatca accgctcacc ggccgctttc tgcaggcaag
    10021 tgttattgaa ccgaattgta gcctgcgtgc acagtatctg gcgtcactac aaacaacgct
    10081 gcaaccctat ccgtggaatg cggttattct tggtttaggt atgggggaac ggttatccgt
    10141 ccccaaagaa atcaaaaata tcatgcgcga tactggaacg gcgcatttaa tggcgatatc
    10201 gggattgcac atcgcttttg cggcgttgct ggctgccgga ctcattcgca gtggacaaat
    10261 ttttctgcct gggcgctgga tccactggca aataccatta attggtggga tctgctgtgc
    10321 tgctttttat gcctggttga cgggaatgca acctcctgca ttgcgtaccg tggtggcgct
    10381 tgctacgtgg ggaatgctta agttaagtgg gcgacagtgg agtggctggg atgtatggat
    10441 atgttgtctg gcggcaattt tgctgatgga tcctgttgcc attctctcgc aaagtttatg
    10501 gctctctgcc gctgcggtcg cggcactgat tttttggtat cagtggtttc cctgtcctga
    10561 gtggcaactg ccgccggtat tgcgtgcagt tgtttccctc atccatctgc aactgggtat
    10621 cacacttctg cttatgcccg tgcaaatcgt catatttcat ggcattagtc tgacctcgtt
    10681 tattgcaaat ctattagcaa ttcccttggt gacatttatc acggttccgt tgatcctcgc
    10741 cgcgatggtt gtgcatttaa gcgggccgtt aatcctggag caagggttat ggtttcttgc
    10801 cgaccggtct ttggctttac ttttctgggg gttaaagagt ttaccagaag ggtggatcaa
    10861 cattgctgaa cgttggcaat ggctatcatt ttccccatgg ttcttactgg tggtatggcg
    10921 attaaacgcc tggcgaacgt tgccagcaat gtgtgtggct gtaggcttgc tgatgtgctg
    10981 gccgctatgg caaaaacctc gacctgacga gtggcaggtg tacatgcttg atgtcgggca
    11041 agggctggca atggtgatag ccagaaacgg caaagcgatt ctctatgaca caggactggc
    11101 ctggcccgaa ggggatagtg ggcaacaact gattatcccc tggctccact ggcataatct
    11161 tgaaccggaa ggcgttattc tgagtcatga acatctggat caccggggag ggctggactc
    11221 aatattgcac acatggccga tgttatggat cagaagtccg ttaaactggg aacatcatca
    11281 gccctgtgtg cgtggcgaag cgtggcaatg gcaaggattg cgtttcagcg tgcactggcc
    11341 tttacaagct agcaacgata aaggaaataa ccattcctgt gtggttaagg ttgatgacgg
    11401 gacgaatagc attcttctaa ccggtgatat tgaagtcccc gctgaacaaa agatgctaag
    11461 ccgttactgg cagcaagtgc agacaacatt gcttcaggta cctcaccatg gcagtaatac
    11521 ctcatcatcg ttgccattaa ttcagcgagt gaatggaaaa gtggcactcg catcggcatc
    11581 gcgctataac gcatggcgat tgccctctaa taaagttaag catcgctatc aacagcaagg
    11641 atatcaatgg cttgatactc cacatcaggg tcaagtgacg gtcaattttt cagcgcaagg
    11701 ctggcggatt agcagcctca gagagcaaat tttacctcgt tggtatcatc agtggtttgg
    11761 cgtgccagtg gataacgggt agaatatgcg gctatttcaa caaatgctgg ttttttgaat
    11821 gcataacgac aaagatctct ctacgtggca gacgttccgc cgactgtggc caaccattgc
    11881 gccttttaaa gcgggtctga tcgtggcggg cgtagcgtta atcctcaacg cagccagcga
    11941 taccttcatg ttatcgctcc ttaagccact tcttgatgat ggctttggta aaacagatcg
    12001 ctccgtgctg gtgtggatgc cgctggtggt gatcgggctg atgattttac gtggtatcac
    12061 cagctatgtc tccagctact gtatctcctg ggtatcagga aaggtggtaa tgaccatgcg
    12121 tcgccgcctg tttggtcaca tgatgggaat gccggtttca ttttttgaca aacagtcaac
    12181 ggggacgctg ttgtcacgta tcacctacga ttccgaacag gttgcttctt cttcttccgg
    12241 cgcactgata acggttgtgc gtgaaggtgc gtcaatcatc ggcctgttca tcatgatgtt
    12301 ctattacagt tggcaactgt cgatcatttt gattgtgctg gcaccgattg tttcgattgc
    12361 gattcgcgta gtatcgaagc gttttcgcaa catcagtaaa aacatgcaga acaccatggg
    12421 gcaggtgacc accagcgccg aacaaatgct gaagggccac aaagaagtat tgattttcgg
    12481 tggtcaggaa gtggaaacga aacgctttga taaagtcagc aaccgaatgc gtcttcaggg
    12541 gatgaaaatg gtttcagcct cttccatctc tgatccgatc attcagctga tcgcctcttt
    12601 ggcgctggcg tttgttctgt atgcggcgag cttcccaagt gtcatggata gcctgactgc
    12661 cggtacgatt accgttgttt tctcttcaat gattgcactg atgcgtccgc tgaaatcact
    12721 gaccaacgtt aacgcccagt tccagcgcgg tatggcggct tgtcagacgc tgtttaccat
    12781 tctggacagt gagcaggaga aagacgaagg taagcgcgtg atcgagcgtg cgactggcga
    12841 cgtggaattc cgcaatgtca cctttactta tccgggacgt gacgtacctg cattgcgtaa
    12901 catcaacctg aaaattccgg cagggaagac ggttgctctg gttggacgct ctggttcagg
    12961 taaatcaacc atcgccagcc tgatcactcg tttttacgat attgatgaag gcgaaatcct
    13021 gatggatggt cacgatctgc gcgagtatac cctggcgtcg ttacgtaacc aggttgctct
    13081 ggtgtcgcag aatgtccatc tgtttaacga tacggttgct aacaacattg cttacgcacg
    13141 gactgaacag tacagccgtg agcaaattga agaagcggcg cgtatggcct acgccatgga
    13201 cttcatcaat aagatggata acggtctcga tacagtgatt ggtgaaaacg gcgtgctgct
    13261 ctctggcggt cagcgtcagc gtattgctat cgctcgagcc ttgctgcgtg atagcccgat
    13321 tctgattctg gatgaagcta cctcggctct ggataccgaa tccgaacgtg cgattcaggc
    13381 ggcactggat gagttgcaga aaaaccgtac ctctctggtg attgcccacc gcttgtctac
    13441 cattgaaaag gcagacgaaa tcgtggtcgt cgaggatggt gtcattgtgg aacgcggtac
    13501 gcataacgat ttgcttgagc accgcggcgt ttacgcgcaa cttcacaaaa tgcagtttgg
    13561 ccaatgatcg aaaaaatctg gtctggtgaa tcccctttgt ggcggctatt gctgccactc
    13621 tcctggttgt atggcctggt aagtggcgcg atccgtcttt gctataaact aaaactgaag
    13681 cgcgcctggc gtgcccccgt accggttgtc gtggttggta atctcaccgc aggcggcaac
    13741 ggaaaaaccc cggtcgttgt ctggctggtg gaacagttgc aacagcgcgg tattcgcgtg
    13801 ggggtcgtat cgcggggata tggtggtaag gctgaatctt atccgctgtt attgtcggca
    13861 gataccacaa cagcacaggc gggtgatgaa cctgtgttga tttatcaacg cactgatgcg
    13921 cctgttgcgg tttctcccgt tcgttctgat gcggtaaaag ccattctggc gcaacaccct
    13981 gatgtgcaga tcatcgtaac cgacgacggt ttacagcatt accgtctggc gcgtgatgtg
    14041 gaaattgtcg ttattgatgg tgtgcgtcgc tttggcaatg gctggtggtt gccggcgggg
    14101 ccaatgcgtg agcgagcggg gcgcttaaag tcggttgatg cggtaatcgt caacggcggt
    14161 gtccctcgca gcggtgaaat ccccatgcat ctgctgccgg gtcaggcggt gaatttacgt
    14221 accggtacgc gttgtgacgt tgctcagctt gaacatgtgg tggcgatggc agggattggg
    14281 catccgccgc gcttttttgc cacgctgaag atgtgcggcg tacaaccgga aaaatgtgta
    14341 ccgctggccg atcatcagtc tttgaaccat gcggatgtca gcgcgttggt aagcaccggg
    14401 caaacgctgg taatgactga aaaagatgcg gtgaaatgcc gggcctttgc agaagaaaat
    14461 tggtggtatt tgcctgtaga cgcacagctt tcaggtgatg aaccagcgaa actgcttacg
    14521 caactaacct cgctggcttc tggcaactag ttacgccgcg gcagcgttcg attgatggag
    14581 tcatgaatgt cgctgccgca cctctccctt gctgatgcgc gtaatcttca ccttgccgca
    14641 caaggcctgt taaacaaacc ccgccgtcaa gcgtcgttgg aggatattcc ggcaacgatc
    14701 tcccgcatgt ccttgctgca aatcgatacc atcaatattg ttgcccgtag tccatatctg
    14761 gtgcttttca gtcgtctggg aaattatcct gcccagtggc tggatgagtc tctggcgcgt
    14821 ggcgaattaa tggaatactg ggcgcatgaa gcctgcttta tgccgcgtag cgactttcgt
    14881 cttattcgcc accgcatgct ggcacctgaa aaaatgggct ggaaatacaa agacgcctgg
    14941 atgcaggaac atgaggcgga aattgcacag ttaattcagc atattcatga taaggggccg
    15001 gtacgttcag ccgattttga gcatcctcgt aaaggtgcaa gcggctggtg ggaatggaag
    15061 ccgcataaac ggcatctgga aggtttattt actgccggaa aggtgatggt gattgaacgg
    15121 cgcaacttcc agcgcgttta tgatttaacc caccgtgtca tgcctgactg ggatgatgag
    15181 cgcgatctcg tttcgcaaac agaagcagaa atcatcatgc tggataacag tgcgcgtagc
    15241 ctgggaatat tccgcgaaca gtggctggca gattactatc ggctgaaacg tccggcactg
    15301 gcggcgtggc gcgaagcgag ggctgaacag cagcaaatca ttgctgtgca tgttgaaaaa
    15361 ttgggcaatc tttggctgca tgctgatttg ctgccgctac tcgagcgtgc gctggccgga
    15421 aagctcactg caacgcacag cgcggtactt tcgccttttg atcctgttgt ctgggatcgc
    15481 aaacgcgcag agcagctttt tgattttagc taccggctgg agtgctatac cccagcgccg
    15541 aaacgccagt atggctattt tgttctgcca ttattacatc gtgggcaatt agttgggcga
    15601 atggatgcca aaatgcatcg ccagacaggc atccttgaag ttatctctct gtggttacag
    15661 gaaggcatta aaccaacgac aatgctgcaa aaagggttac gtcaggcgat tactgatttc
    15721 gcgagctggc agcaggcaac gcgggtgaca ttaggacgct gcccgcaagg tctctttact
    15781 gattgccgca ctggctggga aatagacccc gtcgcataaa tgattatgat aagatttatg
    15841 cattcataac ccgatccatc tggaggaact atggatcatc gtttgcttga aatcattgcc
    15901 tgcccggttt gcaacggaaa actttggtat aaccaggaaa aacaagagct tatttgcaaa
    15961 ctggataacc tggctttccc gctccgcgat ggcattccag tgttgctgga aacggaagcc
    16021 cgcgtgctga ctgctgatga gagtaaatca tgagttttgt ggtcattatt cccgcgcgct
    16081 acgcgtccac gcgtctgccc ggtaaaccat tggttgatat taacggcaaa cccatgattg
    16141 ttcatgttct tgaacgcgcg cgtgaatcag gtgccgagcg catcatcgtg gcaaccgatc
    16201 atgaggatgt tgcccgcgcc gttgaagccg ctggcggtga agtatgtatg acgcgcgccg
    16261 atcatcagtc aggaacagaa cgtctggcgg aagttgtcga aaaatgcgca ttcagcgacg
    16321 acacggtgat cgttaatgtg cagggtgatg aaccgatgat ccctgcgaca atcattcgtc
    16381 aggttgctga taacctcgct cagcgtcagg tgggtatggc gactctggcg gtgccaatcc
    16441 acaatgcgga agaagcgttt aacccgaatg cggtgaaagt ggttctcgac gctgaagggt
    16501 atgcactgta cttctcacgc gccaccattc cttgggatcg tgatcgtttt gcagaaggcc
    16561 ttgaaaccgt tggcgataac ttcctgcgtc atcttggtat ttatggctac cgtgcaggct
    16621 ttatccgtcg ttacgtcaac tggcaggcaa gtccgttaga acacatcgaa atgttagagc
    16681 agcttcgtgt tctgtggtac ggcgaaaaaa tccatgttgc tgttgctcag gaagttcctg
    16741 gcacaggtgt ggatacccct gaagatcttg agcgcgttcg cgctgaaatg cgctaatttc
    16801 acttcacgac acttcagcca attttgggag gagtgtcgta ccgttacgat tttcctcaat
    16861 ttttcttttc aacaattgat ctcattcagg tgacatcttt tatattggcg ctcattatga
    16921 aagcagtagc ttttatgagg gtaatctgaa tggaacagct gcgtgccgaa ttaagccatt
    16981 tactgggcga aaaactcagt cgtattgagt gcgtcaatga aaaagcggat acggcgttgt
    17041 gggctttgta tgacagccag ggaaacccaa tgccgttaat ggcaagaagc tttagtacgc
    17101 ccggaaaagc ccgacaactg gcatggaaaa ccaccatgct ggcaagaagt gggactgtcc
    17161 gtatgccgac tatttatggt gtgatgacgc atgaagaaca ccccggcccc gatgtcctgc
    17221 tactggagcg gatgcgtggt gtttcggtgg aggcaccagc ccgaacacca gaacgctggg
    17281 aacaactcaa agaccaaatc gttgaagcct tactggcctg gcaccgtcag gacagtcgcg
    17341 gttgcgtcgg cgcggtcgac aatactcagg aaaatttctg gccctcatgg taccggcaac
    17401 atgttgaagt gctatggacc acgctcaatc agttcaataa caccggtctg acgatgcagg
    17461 ataagcggat cctgtttcgc actcgcgaat gtctcccggc attatttgaa ggctttaacg
    17521 acaattgtgt gttgattcac ggtaacttct gtttacgcag catgctgaaa gattcgcgca
    17581 gcgatcagtt actggcgatg gtcgggccgg gactaatgct ttgggcaccg cgagaatacg
    17641 aactgttccg actaatggat aattctctgg cggaagattt gctctggagt tacctgcaac
    17701 gcgcgccagt ggcggagtcg ttcatctggc ggcgttggtt gtatgtgtta tgggatgaag
    17761 ttgcgcaact ggttaatacc ggacgattta gtcggcgcaa cttcgatctg gcatcaaaat
    17821 cactcttgcc gtggctcgcc tgacggacct ttcagccact gccagatgcg gccaagcgtt
    17881 tcatagccaa cacgatcgct atgcatcagc cagaccggag aagggattgc ccgttcccac
    17941 ggattaagcg gcgagtcgat ggccaactga ttcgccgggg ctggtagcgg atttaaccct
    18001 tcctgctgaa aaaagatcat cgcccgcggc agatgtgatg cggaggtcac cagcaggaaa
    18061 ggggcgtcac caatcgcctg tttcactgcc gcagcttctt cttcggtatc ttttggcaaa
    18121 tccagggtga taatttgctc gcgcggcacg cccagcgatt gcgcaactct ggcacctact
    18181 tccgctgtac tcaccgtatt ggtttttgct acgcctcccg tgaagatcag ttttgatccc
    18241 ggattttcgc gccataagcg aataccttca ttcaggcgtg gcagactgtt attgattaaa
    18301 ttagagctcg gtgcccactg cgggttccag gtataaccac ctccgagcac cacgatatag
    18361 tccacttttt gcgaattatt ccatgtcgga taggtgcttt cgatgggacg cagcaggcgg
    18421 tcggctaccg gttgcaaact caataacaag agtgccagcc acccaatgct aataaaaatc
    18481 ttcccggttt tttgaaaacg gctaaaccac agcagcgcca ggccagcgcc aataattaac
    18541 aacatcagcg gaagtggcag caacatattg ccaatcactt ttttcagtgt aaaaagcatc
    18601 ctttctggtt ccttttttaa ccatatagca agggatcgcc agcgatatta caccagacgg
    18661 gttcattctc gccgtggctg tgacaaaata gcgtctttat cagaaaacgt cttagccaac
    18721 cggtggagaa agcaatgcag gatcgcaatt ttgatgatat tgcggaaaag ttttcccgta
    18781 acatttacgg caccaccaaa gggcagcttc gacaggctat tctgtggcag gatctcgatc
    18841 acgtgctggc ggaaatgggc ccgcaaaaac tgcgtgtgct ggatgctggc ggtggagaag
    18901 ggcagaccgc aatcaaaatg gccgagcgtg ggcatcaggt cattttatgc gatctttctg
    18961 cgcagatgat cgaccgcgca aaacaggcgg cagaagcaaa aggtgtgagc gacaacatgc
    19021 aatttataca ttgcgccgct caggatgttg cttcgcattt ggaaacgccc gttgatctga
    19081 tattgttcca tgcggtgctc gagtgggtgg ccgatccccg cagtgtattg cagaccctgt
    19141 ggtcagtgtt gcgtccaggc ggcgtgttgt cgttaatgtt ctacaatgcg catggtttgt
    19201 tgatgcataa catggtcgcc gggaattttg attacgtgca ggcgggaatg ccgaaaaaga
    19261 aaaaacggac gctttcgcca gattatccac gcgacccggc gcaggtttat ctgtggctgg
    19321 aagaagctgg ttggcaaatt atgggtaaga caggcgttcg cgtgtttcat gattatctgc
    19381 gcgagaagca ccagcagcgc gactgctatg aagcattact tgaattagaa acgcgttatt
    19441 gccgtcagga accgtatatt accctggggc gttatattca tgtcaccgcg cgcaaaccgc
    19501 agagcaagga taaagtatga gtgaattttc ccagacagtc cccgaactgg ttgcctgggc
    19561 cagaaaaaat gacttctcca tctcgctgcc ggtagaccga ctctcttttc tgctggcggt
    19621 tgccacgctg aacggcgagc gtctggatgg tgagatgagt gaaggcgagc tggtggatgc
    19681 attccgccat gtgagtgatg cgtttgagca aaccagcgaa accatcggcg tgcgcgccaa
    19741 taacgcgatc aacgacatgg tgcgtcaacg tctgctgaac cgctttacca gcgagcaggc
    19801 ggaagggaac gcaatttacc gtctgacgcc gctcggcatc ggcattactg actactacat
    19861 ccgtcagcgc gagttttcta cgctgcgtct ttctatgcag ttgtcgattg tggcgggtga
    19921 gctcaaacgc gcagcagatg ccgccgaaga gggcggtgat gaatttcact ggcaccgtaa
    19981 tgtctatgcg ccactgaaat attcggtagc agaaattttc gacagtatcg acctgacgca
    20041 acgtctgatg gacgaacagc agcagcaggt gaaggacgat atcgcccagt tgctgaacaa
    20101 agactggcgg gcggcgattt ccagctgtga attgttgctt tcggaaactt ccggaacgct
    20161 gcgtgaattg caggatacgc tggaagcggc aggcgacaaa ttgcaggcta atctgttgcg
    20221 cattcaggat gcgacgatga cccatgacga tctgcatttc gtcgatcgtc tggtgttcga
    20281 tctgcagagc aaactcgatc gtattatcag ttggggccag caatccatcg acttgtggat
    20341 tggctacgac cgccacgtac acaaatttat tcgtaccgcg atcgatatgg ataaaaaccg
    20401 cgtctttgct cagcggttac gtcagtcggt acaaacctat tttgatgagc cgtgggcgct
    20461 aacttatgcc aatgccgatc gtctgctgga tatgcgtgac gaagagatgg cactgcgcga
    20521 tgaagaagtg actggggaac ttcctgagga tctggaatac gaagagttta acgagatccg
    20581 cgaacagctg gcggcgatca tcgaagaaca acttgccgtg tacaaaacca gacaagtgcc
    20641 gctggatctt ggtctggtgg tacgcgaata tctgtcacag tatccgcgtg cacgtcactt
    20701 tgacgttgcg cgcattgtta ttgatcaggc ggtacgtctt ggcgtagcgc aagcagattt
    20761 caccggactg ccagcgaaat ggcagccgat taatgattac ggagccaagg tacaggcgca
    20821 tgtcatcgac aaatattgaa caagtgatgc cggttaagct ggcgcaggcg ctggcgaatc
    20881 cgttatttcc ggcgctggac agcgccttac gttcaggccg ccatattggc ctcgacgaac
    20941 tggataatca tgcattcctg atggattttc aggaatatct ggaagagttt tacgcgcgtt
    21001 ataacgttga gcttattcgc gcaccagaag ggttcttcta tttacgccca cgttccacca
    21061 cgctgatccc tcgttccgtc ttgtcggaac tggatatgat ggtcgggaaa atcctctgtt
    21121 atctctatct cagcccggaa cggctggcga atgaggggat tttcacccag caggaactgt
    21181 acgacgaact gctcaccctg gccgatgaag caaaactgct gaaactggtg aacaaccgtt
    21241 caaccggttc agacgttgac cgtcagaagt tgcaggagaa agtacgttct tcgctcaacc
    21301 gtctgcgtcg tttaggcatg gtgtggttta tgggccacga cagcagcaag tttcgcatta
    21361 ccgaatcggt gttccgcttc ggagccgatg tgcgtgctgg cgacgatccc cgtgaagcac
    21421 agcgtcgcct gattcgtgat ggcgaagcaa tgccgattga aaatcatctg caactcaacg
    21481 atgaaaccga agagaatcag ccagatagcg gagaggaaga ataatgattg aacgcggtaa
    21541 atttcgctca ctgacgctga ttaactggaa cggctttttt gcccgaactt ttgaccttga
    21601 cgagctggtc acgacgcttt ctggcggtaa cggggcgggt aaatccacca ccatggcggc
    21661 gttcgttacg gcgctgatcc ccgacctgac cctgctgcat ttccgtaaca ctacggaagc
    21721 cggggccacc agcggttcgc gcgataaagg tctgcacggt aagctgaaag cgggtgtctg
    21781 ttattcgatg ctcgacacca ttaactcgcg ccaccagcgc gtggtggtcg gtgtgcgtct
    21841 gcaacaggtt gccggacgcg atcgtaaagt ggatatcaag ccgtttgcca ttcagggact
    21901 gccgatgtcg gtgcagccga cacagctggt gaccgaaacc ctgaacgaac gccaggcgcg
    21961 cgtgctgccg cttaacgagc tgaaagacaa gctcgaggcg atggaaggcg tgcagtttaa
    22021 acagttcaac tccattactg attaccactc gctgatgttc gatctgggca tcatcgcgcg
    22081 tcgtctgcgc tccgcatctg accgtagcaa attctatcgt ctgatcgaag cttcgctgta
    22141 tggcgggatc tccagtgcca ttacccgttc tctgcgcgac tacctgttgc cggaaaacag
    22201 cggcgtgcgt aaagcgttcc aggacatgga agcggcgctg cgtgaaaacc gtatgacgct
    22261 ggaagcgatt cgtgtcaccc agtcggaccg cgacctgttt aagcatctga tcagcgaagc
    22321 caccaactac gtggcggcgg actacatgcg tcacgccaac gagcgccgtg tccatctcga
    22381 caaagccctg gagtttcgtc gcgagctaca tacttcgcgt cagcaactgg cggctgagca
    22441 gtacaaacac gtcgatatgg cgcgtgagct ggcagagcac aacggtgccg aaggcgatct
    22501 ggaagcggat tatcaggcgg ccagcgatca cctgaacctg gtgcaaaccg cactgcgtca
    22561 gcaggagaag atcgaacgct acgaagcgga tctcgatgag ctgcagatcc gtctggaaga
    22621 gcaaaatgaa gtggtggcag aagccatcga acgccaggaa gagaatgagg ctcgtgcgga
    22681 agctgccgaa ctggaagtgg acgagctgaa aagccagctt gctgactacc agcaggcgct
    22741 ggacgtccag caaacgcgcg cgatccagta taaccaggcg attgctgcgc ttaatcgtgc
    22801 caaagaactg tgccatctgc cggacttaac cgccgactgc gccgccgaat ggctggaaac
    22861 cttccaggcg aaagagctgg aagcgactga aaaaatgctc tctcttgagc agaaaatgag
    22921 catggcgcaa accgcgcaca gccagtttga gcaggcttat cagctggtgg tggcaatcaa
    22981 cggcccgctg gcgcgtaacg aggcgtggga tgtcgctcgc gaactattgc gcgaaggggt
    23041 cgatcagcgt cacctggcag agcaggttca accgttgcgg atgcgcttaa gtgaactgga
    23101 acagcgtctg cgcgagcagc aagaagccga acgcctgctg gcagacttct gcaaacgtca
    23161 gggcaagaat tttgatattg atgaactgga agctctgcat caggagctgg aagcgcgcat
    23221 tgcctctctt tccgatagcg tgtctaacgc ccgtgaagag cgcatggcac tgcgccagga
    23281 acaggaacag ctgcagtctc gcattcagag tttgatgcag cgtgcgccgg tttggctggc
    23341 agcgcaaaac agtctcaacc agttgagcga gcagtgcggt gaagagttta cctccagcca
    23401 ggacgtcacc gaatatctgc aacagttgct ggagcgtgag cgagaggcga ttgttgaacg
    23461 cgatgaagtg ggcgcgcgca aaaacgctgt cgatgaagag atcgaacgtt taagccagcc
    23521 tggcggctct gaagatcagc gtctgaacgc gctggcggag cgttttggtg gtgtgctgct
    23581 gtcagaaatt tatgacgacg ttagcctgga agatgcgccg tacttctcag cgctgtatgg
    23641 cccgtcacgc cacgccatcg tggtgccaga tctgtcacag gtaactgaac acctggaagg
    23701 cttgaccgat tgcccggaag atctctatct gatcgaagga gatccgcagt cattcgatga
    23761 cagcgtgttc agcgttgatg agctggaaaa agcggtagtg gtgaaaatcg ccgatcgtca
    23821 gtggcgttat tcacgtttcc cggaagtgcc gctgtttggt cgtgctgcgc gtgaaagccg
    23881 tattgaaagc ctccatgccg agcgtgaagt gctttccgaa cgcttcgcca cgctctcctt
    23941 tgatgtacag aaaactcagc gtctgcatca ggcgttcagc cgctttatcg gcagtcatct
    24001 ggcggttgcg tttgagtctg acccggaagc agaaatccgt caactgaaca gccgtcgcgt
    24061 cgaactggag cgggcgttaa gtaatcatga aaatgataac cagcagcagc gtattcagtt
    24121 tgagcaggcg aaagagggcg ttacggcgct gaaccgcatt ctgccgcgtc tcaacctgtt
    24181 ggctgatgac agcctggcgg atcgcgtcga tgaaatccgc gaacgtctgg atgaagccca
    24241 ggaagccgcg cgttttgttc agcagtttgg caatcaactg gcgaaactgg aaccgattgt
    24301 ttcggtattg cagagcgacc cggaacagtt cgaacagtta aaagaagatt acgcgtactc
    24361 tcagcagatg cagcgcgatg cccgtcagca ggcgtttgcc ctgacggaag tggtgcagcg
    24421 tcgtgcgcac tttagctatt ctgactcggc agaaatgctt agcggtaaca gcgatctcaa
    24481 cgaaaaactg cgtgaacgtc tggaacaggc ggaagcggag cgtacccgcg ctcgcgaagc
    24541 gttgcgcggt cacgcagcgc agttgagtca gtacaaccag gtgctggctt cgctgaaaag
    24601 ttcttacgac accaaaaaag agctactcaa cgatctgcaa cgtgaattgc aggatatcgg
    24661 cgtgcgtgct gatagcgggg cagaagagcg ggcgcgtatt cgccgtgacg agctgcatgc
    24721 gcaactgagc aataaccgtt cacgccgcaa tcaactggaa aaagcgctta ccttctgcga
    24781 agcggagatg gacaacctga cccgcaaact gcgcaagctg gagcgggatt actttgagat
    24841 gcgcgagcag gtagtgaccg ccaaagcggg ctggtgtgcg gtgatgcgca tggtgaaaga
    24901 taacggcgtt gagcgccgct tacaccgtcg tgagctggct tatctctccg ctgatgattt
    24961 gcgttccatg tcggataagg cgttaggtgc gctgcgtctg gcggtggcgg ataacgaaca
    25021 tctgcgcgac gtgctgcgca tgtcggaaga tccgaaacgt ccggagcgta aaattcagtt
    25081 cttcgtggcg gtttatcagc atctgcgtga acgtattcgt caggatatta ttcgtaccga
    25141 tgatccggtg gaagctatcg aacagatgga gattgaactt agccgtctga ccgaagaatt
    25201 aacctcccgt gaacagaaac tggcgatcag ttcccgcagc gtggcgaaca tcattcgcaa
    25261 aaccattcag cgcgagcaga accgtatccg tatgctcaac caggggttgc agaacgtatc
    25321 gtttggtcag gtgaacagcg tgcgtctcaa cgtgaacgtg cgtgaaacgc acgccatgct
    25381 actggatgtg ctctctgaac agcacgagca gcatcaggat ctgtttaaca gcaaccgttt
    25441 gaccttctcg gaagcgctgg cgaaactgta tcaacgtctt aacccgcaga ttgatatggg
    25501 gcagcgcacg ccgcagacca tcggtgaaga actgctggat taccgcaact atctggaaat
    25561 ggaagttgag gttaaccgtg gttccgatgg ctggctgcgc gcagagtctg gtgcattgtc
    25621 gaccggtgag gcgattggta ccggtatgtc gattctggtg atggtggtac aaagctggga
    25681 agatgaatct cgccgcctgc gcggtaaaga tatctctcct tgccgcctgc tgttcctcga
    25741 tgaagcagcg cgactggatg ctcgttctat cgccacgctg tttgaattgt gtgaacgttt
    25801 gcaaatgcag ctcatcatcg cagcgccgga aaatatcagc ccggagaaag gcaccaccta
    25861 taaactggtg cgtaaagtct tccagaatac cgaacacgtt cacgtcgtcg gcctgcgagg
    25921 atttgcgccg caactccctg aaacgcttcc aggaagtgac gaagcgcctt ctcaggcgag
    25981 ttaaaattaa ggcggcagca atgccgcctt ttctttttcc gaaaactccg tttctgcact
    26041 aaaaaagtgg cacattacgg cgcgcttttc tttaaacttc tttacattag gttatgtaaa
    26101 aacgtatcgg cgtttatata ctgaagataa gcctgatgag taacaggctt gctcgtcata
    26161 ctttcgtgag tattggcgtt gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag
    26221 tatgataaaa acagggggca agggatgttg cttaatatga tgtgtggtcg tcggctgtcg
    26281 gcaatcagtt tgtgcctggc cgtaacattc gctccactgt tcaatgcgca ggccgatgag
    26341 cctgaagtaa tccctggcga cagcccggtg gctgtcagtg aacagggcga ggcactgccg
    26401 caggcgcaag ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggcagaa
    26461 aaagcccgca cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac
    26521 cagcttcaac tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct
    26581 gttaaagcct tccagcaaca gctggcagag gtggcgattg ccggtttcca gccgcagttt
    26641 aataaatggg tagagttact gaccgatcct ggtgttaacg ggatggcacg cgacgtagtg
    26701 ctctctgatg cgatgatggg ctatctccat ttcattgcaa atattccggt caaaggcact
    26761 cgctggctat atagcagtaa accttatgca cttgcaacgc cgccgctctc ggtgattaac
    26821 caatggcagc tggcgctgga taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg
    26881 cagcatccgc aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa
    26941 ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta
    27001 ccggcgttgc gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact
    27061 ctacctggcg atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa
    27121 acagcagaaa ctaagccgat ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc
    27181 gttcgtgccg cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa
    27241 ggattggggg cagatggtgc tattggcccg gcaacacgtg actggttaaa cgtaacgccc
    27301 gcccagcgtg ctggcgtgtt ggctctcaac atccagcgat tgcgcttgct gccaacagag
    27361 ctttctaccg ggatcatggt taacattccg gcctattcgc tggtctacta tcagaacggc
    27421 aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg
    27481 agcagtgccc ttaacaacgt agtggtaaac ccgccgtgga acgtacctcc aactctggca
    27541 cgcaaagata ttctgccaaa agtgcgcaac gatccgggat atctcgaaag ccatggctat
    27601 acggtgatgc gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct
    27661 acaatcacgg cctcgaattt accgttccgc ttccagcagg ctccaggccc acggaactcg
    27721 ctggggcgct ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg
    27781 aaccacaatc tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat
    27841 aaagcttccg atctggcgaa tatgctgttg caggatgcag gctggaatga caaacgtatt
    27901 tctgatgcgc tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg
    27961 aatctctact acctgacggc ctttgttggt gcagatggtc gtacccagta tcgtacagat
    28021 atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa
    28081 ttaatcaggt aaatgaagta gttcggggaa atcagttgtc gtaataatta gcatgattgg
    28141 gggcgattct ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac
    28201 gggcggttaa ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta
    28261 gacttgatta tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc
    28321 gttgcactcg gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc
    28381 ccgcgcattt tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc
    28441 gatggcagag gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac
    28501 cgcgcgaaca aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa
    28561 gggttgttag gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc tattgatacc
    28621 aacaatgaac tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc
    28681 caggcgatgg atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta
    28741 tctatgcgcg caggtggtgt aggatattat ccacgtagta actttgtgca tattgatacc
    28801 gggccagcac ggcactggta gtaattgctt aacgaaacag gggcagtatg aactatcgta
    28861 ttattccggt caccgcattc tcccagaact gttcattaat ctggtgtgaa caaacccgtc
    28921 tggccgcact ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca
    28981 gcggcctgac actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag
    29041 cggcggaact ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt
    29101 tctggctgca aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc
    29161 tgacgccaga tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac
    29221 aggtgttaca ttgccctggg catacgccgg gtcatgtcgt gttttttgat gatcgggcaa
    29281 agctgctgat ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc
    29341 gtggcgatca taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg
    29401 acgtgacatt tattccgggt cacggaccat tatccacact tggttatgaa cgcctgcata
    29461 atcccttcct gcaagacgaa atgcccgtct ggtaaggcac ataaaaaagc ccgcttttaa
    29521 tgcgggcctg gatttctggc aaagtgcgct ttgtttatgc cggatgcggc acgagcgcct
    29581 tatccggcct acaaaatcgt gcaaattcaa aatattgcag gggacgcgta ggcctgataa
    29641 gcgtagcgca tcaggcaatg ttgcgtttgt catcagtctc agcccgcttt tcagcgggct
    29701 tcattgtttt taatgcttac agcactgcca caatcgcttc gcacagcgga gccatgttat
    29761 ctggtgtcat cccggccaca tttacgcgac cagaagcaac cgcatatacg ccaaactctt
    29821 cgcgcagacg cagcacttgt tcttttgtca ggccactgaa ggagaacatg ccgttctgtt
    29881 tgatgataaa gctgaagtcg cggtttgcgc ctttttcctg cagcgtattg acgaacaact
    29941 gacgcatacg ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa atcgcacgta
    30001 acgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt
    30061 agttagcgcg aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt tcactgtcgg
    30121 cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt ttagagtagg
    30181 aactggcaac aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct tcagcatctt
    30241 cttccagacc acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac cagcctttct
    30301 caactgagag ttgtgccagt gtctgccatt gttccagcgt agggtcgata ccggttgggt
    30361 tatggcagca gccatggaac agcactacgt cgccagcctg agcttcattc aggctgttaa
    30421 tcagtgcatc gaagtcaaga gtgtgatttt ccgcatcata ataagcgtat tcacgaactt
    30481 ccagacctgc agagttaaag acgctcttat ggttcggcca gcttgggttg ctcacccaca
    30541 ctcgcttaac gctggtattt tttgccagga aatcggcagc tacgcgtagt gcgccagtgc
    30601 cccccggagt ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta cctttaccaa
    30661 acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt
    30721 tggtggtttc attttcgagc agatactgtt cagccttttt cacgctggtc agtaccgggg
    30781 ttttgcccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg ggacgttcat
    30841 cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg gtaatgttct
    30901 caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag ggtaacggga
    30961 gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca agacgtgaga
    31021 ttgctctgga aggtataaaa aaaacaggac caaagtcctg ttttttcggc atttaacaaa
    31081 gaggtatgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc tgaacctacg
    31141 cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt ggacatgttt
    31201 ttgttgaagt agtaggttgc gcccacttca aagtagttca ccagatcaac atcaccgata
    31261 ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag accgaaatcg
    31321 aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt
    31381 gtaaatttat tagtgatcgg cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag
    31441 atgttgttcg cgtcgtactt cagaccagta gcccactgtt cagctttttt accgttgcca
    31501 agaggttgag cttcttgcag gttggtacgg tcagctgcac cataagcacc aacgatacca
    31561 aagccttcgt attcgtagct gatagaaccg ccaacaccgt cgccgttaga acggcgtgca
    31621 gtgtcacgct cgtttttacc caggtactga acagcgaagt tcaggccatc aaccagacca
    31681 aagaagttgg agttacgata ggtagcaacg ccgccaacac gaccaacgaa gaagtcatcg
    31741 ctgtatgcag tatcaccacc aaattctggc agcatatcgg tgtaacccag tgcatcataa
    31801 accacaccgt agttacggcc gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg
    31861 aatgccagac gcgttttgtt accagtttga gcgtcagcgc cttcagagtt gttaccctgg
    31921 aagttatatt cccactgacc ataaccggtc agatcggaat tgatttgagt ttccccttta
    31981 aaaccaagac gggcataggt catgtcgcca ttgccaccgt aactgttttc accgttaccc
    32041 ttggaaaaat aatgcagacc aacagcttta ccgtacagat ctactttgtt gccatctttg
    32101 ttatagattt ctgcagcgtt tgcagtacct gctactaaca gagcagggac gatcactgcc
    32161 agaatattgc gcttcatcat tatttattac cctcatggtt ttttttatga cacctgccac
    32221 tgccgtcaat aagttctgtc aataaaaatt tacggaacta ttgatgagag tttggtgtct
    32281 ttatgtgtct gcaggcatct ttccattcaa actaacgttt cgctaccgtg aaagtgctac
    32341 aaagataaag atttagtttc aaaaagaaaa aatatgtaac caaaagtaaa aattaaggaa
    32401 ctttgtgaac accgtcatat ttccatagaa acgtgatg
//
LOCUS       DAZFVO020000041        31531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFVO020000041 DAZFVO020000000
VERSION     DAZFVO020000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.041"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            139..669
                     /locus_tag="ACHKNB_RS08935"
                     /old_locus_tag="pgaptmp_001787"
     CDS             139..669
                     /locus_tag="ACHKNB_RS08935"
                     /old_locus_tag="pgaptmp_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195317.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            921..1205
                     /locus_tag="ACHKNB_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /pseudo
     CDS             921..1205
                     /locus_tag="ACHKNB_RS08940"
                     /old_locus_tag="pgaptmp_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
     gene            complement(1240..2283)
                     /gene="guaC"
                     /locus_tag="ACHKNB_RS08945"
                     /old_locus_tag="pgaptmp_001789"
     CDS             complement(1240..2283)
                     /gene="guaC"
                     /locus_tag="ACHKNB_RS08945"
                     /old_locus_tag="pgaptmp_001789"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGU4195318.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(2439..2483)
                     /locus_tag="ACHKNB_RS08950"
                     /old_locus_tag="pgaptmp_001790"
     CDS             complement(2439..2483)
                     /locus_tag="ACHKNB_RS08950"
                     /old_locus_tag="pgaptmp_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195319.1"
                     /translation="MIIKTLKMSARKRN"
     gene            2508..3128
                     /gene="coaE"
                     /locus_tag="ACHKNB_RS08955"
                     /old_locus_tag="pgaptmp_001791"
     CDS             2508..3128
                     /gene="coaE"
                     /locus_tag="ACHKNB_RS08955"
                     /old_locus_tag="pgaptmp_001791"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGU4195320.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            3128..3871
                     /gene="zapD"
                     /locus_tag="ACHKNB_RS08960"
                     /old_locus_tag="pgaptmp_001792"
     CDS             3128..3871
                     /gene="zapD"
                     /locus_tag="ACHKNB_RS08960"
                     /old_locus_tag="pgaptmp_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HGU4195321.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            3881..4078
                     /gene="yacG"
                     /locus_tag="ACHKNB_RS08965"
                     /old_locus_tag="pgaptmp_001793"
     CDS             3881..4078
                     /gene="yacG"
                     /locus_tag="ACHKNB_RS08965"
                     /old_locus_tag="pgaptmp_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HGU4195322.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(4178..4567)
                     /gene="mutT"
                     /locus_tag="ACHKNB_RS08970"
                     /old_locus_tag="pgaptmp_001794"
     CDS             complement(4178..4567)
                     /gene="mutT"
                     /locus_tag="ACHKNB_RS08970"
                     /old_locus_tag="pgaptmp_001794"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HGU4195323.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(4627..7332)
                     /gene="secA"
                     /locus_tag="ACHKNB_RS08975"
                     /old_locus_tag="pgaptmp_001795"
     CDS             complement(4627..7332)
                     /gene="secA"
                     /locus_tag="ACHKNB_RS08975"
                     /old_locus_tag="pgaptmp_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGU4195324.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELQDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLCERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(7394..7906)
                     /gene="secM"
                     /locus_tag="ACHKNB_RS08980"
                     /old_locus_tag="pgaptmp_001796"
     CDS             complement(7394..7906)
                     /gene="secM"
                     /locus_tag="ACHKNB_RS08980"
                     /old_locus_tag="pgaptmp_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HGU4195325.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(8137..9054)
                     /gene="lpxC"
                     /locus_tag="ACHKNB_RS08985"
                     /old_locus_tag="pgaptmp_001797"
     CDS             complement(8137..9054)
                     /gene="lpxC"
                     /locus_tag="ACHKNB_RS08985"
                     /old_locus_tag="pgaptmp_001797"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HGU4195326.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(9155..10306)
                     /gene="ftsZ"
                     /locus_tag="ACHKNB_RS08990"
                     /old_locus_tag="pgaptmp_001798"
     CDS             complement(9155..10306)
                     /gene="ftsZ"
                     /locus_tag="ACHKNB_RS08990"
                     /old_locus_tag="pgaptmp_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGU4195327.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(10367..11629)
                     /gene="ftsA"
                     /locus_tag="ACHKNB_RS08995"
                     /old_locus_tag="pgaptmp_001799"
     CDS             complement(10367..11629)
                     /gene="ftsA"
                     /locus_tag="ACHKNB_RS08995"
                     /old_locus_tag="pgaptmp_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGU4195328.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(11626..12456)
                     /gene="ftsQ"
                     /locus_tag="ACHKNB_RS09000"
                     /old_locus_tag="pgaptmp_001800"
     CDS             complement(11626..12456)
                     /gene="ftsQ"
                     /locus_tag="ACHKNB_RS09000"
                     /old_locus_tag="pgaptmp_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HGU4195329.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(12458..13378)
                     /gene="ddlB"
                     /locus_tag="ACHKNB_RS09005"
                     /old_locus_tag="pgaptmp_001801"
     CDS             complement(12458..13378)
                     /gene="ddlB"
                     /locus_tag="ACHKNB_RS09005"
                     /old_locus_tag="pgaptmp_001801"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4195330.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQELVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(13371..14846)
                     /gene="murC"
                     /locus_tag="ACHKNB_RS09010"
                     /old_locus_tag="pgaptmp_001802"
     CDS             complement(13371..14846)
                     /gene="murC"
                     /locus_tag="ACHKNB_RS09010"
                     /old_locus_tag="pgaptmp_001802"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGU4195331.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(14900..15967)
                     /gene="murG"
                     /locus_tag="ACHKNB_RS09015"
                     /old_locus_tag="pgaptmp_001803"
     CDS             complement(14900..15967)
                     /gene="murG"
                     /locus_tag="ACHKNB_RS09015"
                     /old_locus_tag="pgaptmp_001803"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HGU4195332.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPLLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(15964..17208)
                     /gene="ftsW"
                     /locus_tag="ACHKNB_RS09020"
                     /old_locus_tag="pgaptmp_001804"
     CDS             complement(15964..17208)
                     /gene="ftsW"
                     /locus_tag="ACHKNB_RS09020"
                     /old_locus_tag="pgaptmp_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HGU4195333.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(17208..18524)
                     /gene="murD"
                     /locus_tag="ACHKNB_RS09025"
                     /old_locus_tag="pgaptmp_001805"
     CDS             complement(17208..18524)
                     /gene="murD"
                     /locus_tag="ACHKNB_RS09025"
                     /old_locus_tag="pgaptmp_001805"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGU4195334.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(18527..19609)
                     /gene="mraY"
                     /locus_tag="ACHKNB_RS09030"
                     /old_locus_tag="pgaptmp_001806"
     CDS             complement(18527..19609)
                     /gene="mraY"
                     /locus_tag="ACHKNB_RS09030"
                     /old_locus_tag="pgaptmp_001806"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGU4195335.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(19603..20961)
                     /gene="murF"
                     /locus_tag="ACHKNB_RS09035"
                     /old_locus_tag="pgaptmp_001807"
     CDS             complement(19603..20961)
                     /gene="murF"
                     /locus_tag="ACHKNB_RS09035"
                     /old_locus_tag="pgaptmp_001807"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGU4195336.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(20958..22445)
                     /gene="murE"
                     /locus_tag="ACHKNB_RS09040"
                     /old_locus_tag="pgaptmp_001808"
     CDS             complement(20958..22445)
                     /gene="murE"
                     /locus_tag="ACHKNB_RS09040"
                     /old_locus_tag="pgaptmp_001808"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HGU4195337.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(22432..24198)
                     /gene="ftsI"
                     /locus_tag="ACHKNB_RS09045"
                     /old_locus_tag="pgaptmp_001809"
     CDS             complement(22432..24198)
                     /gene="ftsI"
                     /locus_tag="ACHKNB_RS09045"
                     /old_locus_tag="pgaptmp_001809"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HGU4195338.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(24214..24579)
                     /gene="ftsL"
                     /locus_tag="ACHKNB_RS09050"
                     /old_locus_tag="pgaptmp_001810"
     CDS             complement(24214..24579)
                     /gene="ftsL"
                     /locus_tag="ACHKNB_RS09050"
                     /old_locus_tag="pgaptmp_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGU4195339.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(24576..25517)
                     /gene="rsmH"
                     /locus_tag="ACHKNB_RS09055"
                     /old_locus_tag="pgaptmp_001811"
     CDS             complement(24576..25517)
                     /gene="rsmH"
                     /locus_tag="ACHKNB_RS09055"
                     /old_locus_tag="pgaptmp_001811"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGU4195340.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(25519..25977)
                     /gene="mraZ"
                     /locus_tag="ACHKNB_RS09060"
                     /old_locus_tag="pgaptmp_001812"
     CDS             complement(25519..25977)
                     /gene="mraZ"
                     /locus_tag="ACHKNB_RS09060"
                     /old_locus_tag="pgaptmp_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HGU4195341.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(26579..27583)
                     /gene="cra"
                     /locus_tag="ACHKNB_RS09065"
                     /old_locus_tag="pgaptmp_001813"
     CDS             complement(26579..27583)
                     /gene="cra"
                     /locus_tag="ACHKNB_RS09065"
                     /old_locus_tag="pgaptmp_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HGU4195342.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANGPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(27665..27751)
                     /locus_tag="ACHKNB_RS09070"
                     /old_locus_tag="pgaptmp_001814"
     CDS             complement(27665..27751)
                     /locus_tag="ACHKNB_RS09070"
                     /old_locus_tag="pgaptmp_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HGU4195343.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(27763..28254)
                     /gene="ilvN"
                     /locus_tag="ACHKNB_RS09075"
                     /old_locus_tag="pgaptmp_001815"
     CDS             complement(27763..28254)
                     /gene="ilvN"
                     /locus_tag="ACHKNB_RS09075"
                     /old_locus_tag="pgaptmp_001815"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGU4195344.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(28257..29981)
                     /gene="ilvI"
                     /locus_tag="ACHKNB_RS09080"
                     /old_locus_tag="pgaptmp_001816"
     CDS             complement(28257..29981)
                     /gene="ilvI"
                     /locus_tag="ACHKNB_RS09080"
                     /old_locus_tag="pgaptmp_001816"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HGU4195345.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            30100..30144
                     /locus_tag="ACHKNB_RS09085"
                     /old_locus_tag="pgaptmp_001817"
     CDS             30100..30144
                     /locus_tag="ACHKNB_RS09085"
                     /old_locus_tag="pgaptmp_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195346.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(30299..31243)
                     /gene="leuO"
                     /locus_tag="ACHKNB_RS09090"
                     /old_locus_tag="pgaptmp_001818"
     CDS             complement(30299..31243)
                     /gene="leuO"
                     /locus_tag="ACHKNB_RS09090"
                     /old_locus_tag="pgaptmp_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HGU4195347.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7662 a         8771 c         7578 g         7520 t
ORIGIN      
        1 acgggtaaat aatgtatcac caaaaatacc aaataaaggt atttttggta ttttggctaa
       61 tttttgcttt tatccttatt tttcaattgg ataaatagaa agaaaccttt ccttagaaaa
      121 gcaccaagag gtatctttat gtcgcaggga aaagaacctg ttattgaacg tattggaatg
      181 cttacaaagc gctacccttc aagaagtgca gctgccagag cgtggggaat caatatcaac
      241 acattaaaca gctatttcaa gggaggacaa acacaaccag cccccagaga aagtatcctt
      301 atacgaattt ctgaaagtga aggagttagt cttgagtggc ttaaagaggg caagggtgaa
      361 tcaccaaacg atgttggtat ttgtggtaat tttggttgtt ctggtgataa gggagatcaa
      421 cttacacaga tgctttcatt ccttacgaaa gacgaacgag aacagttaac caatctcctt
      481 gctcgcaaag gtgtagagac ggttctgaca ctgctggata aagaaaacca ggatttatta
      541 caactagaag gcaggaagcg cctggctgcg ttgatgattg tagatatgcc agacgattgc
      601 gtcagagaga ttttggaaaa aaccgagaca agtaggcaat caaatccggt agaatctaaa
      661 gcgggttagt ttggcgaggc tggcccgtat gttagttgcc atatagattt aatgaagttt
      721 taaacgcttt aaaaagttga gatcgcagta tcaaaagttt tgccgatttt agaataaacg
      781 attaattatt gcacaatcgg tatcaaatag actttaagcg ctcatccaga aatcacctgt
      841 tttatttcag tatcttacag gtgatttcac ctcctttcac taaaaccctc gttggtatca
      901 aatgattcac ctggttacat atcagtaacg gtcatcccat ctggctggcg ctaaaaaatg
      961 ccggtgagtt tagcccgctc tgtttgcaat tagtgagaac gggagaggca tccggctcgc
     1021 tggatctcat gttagacaac ctcgcccatc atcatcggga taacacaatg gcgctggcgg
     1081 ataacctcgc agccttactg gaaccggcgt tgctgatcat aacgggagga attatcggta
     1141 cgctggtggt ggcgatgtat ctgccaattt tccatttagg cgatgcgatg agtgggatgg
     1201 gataacgctg gcgtgttgct ccacgccagc gttgggagat tacaggttgt tgaagatgcg
     1261 gttttcttgt tcctgcaccc gaataaacgt ggtgcgcttg gtcagttctt tcaggcgtga
     1321 agccccaacg tatgtacaag ctgaacgcaa gccgcccaaa atatcacgcg cggtattttc
     1381 aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat attccgcaac
     1441 gccgccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca gcataaattt
     1501 ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc
     1561 aagcatgacg aaatcggcac cgccgccaaa ggctttcgcg acatcgcccg gcgtggtgca
     1621 gccaccgtcg ctgacgatca ttccgcccag accgtgcgcc gcatcggcac attcgattac
     1681 cgcagaaagc tgcggataac cgacgcctgt tttgacacga gttgtacaaa cagaacctgg
     1741 gccaatgcca actttaacga tatcagcacc ggagaggata agctcctcac acatttcacc
     1801 agtcactacg ttgcccgcac aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac
     1861 gaactgcacg aagtgttcgg aataaccatt cgccacatca atacaaacga agtttaatgc
     1921 cgggttcagg tcgagaattt gtttagtttt ttcgaaatcc gcatcagacg taccggtaga
     1981 aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt gccactcttc
     2041 gacagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg cagaggccat
     2101 agaaaatgta cctacggtgt ccatatttgc ggcgataatc ggcacgccgg accagctctg
     2161 acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc ttttaagagt
     2221 ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt cttcaatacg
     2281 catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc agtgacgcta
     2341 tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta cgctacaatc
     2401 ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt tcgtgcactc
     2461 atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg aggtatatag
     2521 ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg tttgctgatc
     2581 tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac
     2641 ctgcgctaca tgccattgct gatcactttg gcgctaacat gattgctgct gatggaacat
     2701 tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa aactggctta
     2761 acgccctgct gcatccgctg attcagcaag agacgcaaca ccagatccag caagctactt
     2821 ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga
     2881 atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc atgcagcgcg
     2941 atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaacg cgcgaagccc
     3001 gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct atcgcatcgg
     3061 atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa
     3121 aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa tgcgtacatg
     3181 gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg ttgaccacgc
     3241 tggcgcgctg catttcttcc gtaatgtcag tgaattactg gatgttttcg agcgcggcga
     3301 agtccgcact gagctgttga aagaacttga ccggcagcaa cgtaaactcc agacctggat
     3361 tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt taaaagcggc
     3421 ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag atcgtttgat
     3481 tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg atttacctac
     3541 attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag aaacctggat
     3601 tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa ttcgccagtc
     3661 ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg gtggcgatgc
     3721 cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa tttccggaca
     3781 taagagccgt tttgccattc gttttatgcc gctggacact gaaaacggac aggtaccgga
     3841 acgtctggat ttcgaactgg cctgttgcta aggagtgaaa atgtcagaaa ctattacggt
     3901 gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt ttcggccatt
     3961 ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag aaaaacgaat
     4021 ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagagccaa agcagtgaca
     4081 tttgtctgat gccgcacgta ggcctgataa gacgcggaca gcgtcgcatc aggcatcttg
     4141 tgcaaatgtc ggatgcggcg tgaacgcctt atccgaccta cagacgctta agcttcgcaa
     4201 ttaccggttc attggctggc ggaaaatcat cggcattaag accgactagc gacatccact
     4261 caccgggttg cccttcttta ccccacggct ccccttccca gctttcgacc agccagaacc
     4321 acagagttat atgcctgtcc gggaattcat attccagttt ttcaaatagc gaaaaatgtt
     4381 gtggggtaat cccgacttct tcctgaagtt cacgcaccac cgcctgttcc ggcgtttcgc
     4441 ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca tctgctgcgc
     4501 gacgcgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca atttgcagct
     4561 ttttcattgt cttaaaccta taaaaaaggc gcagaatcct gcgcctttta cttcaactgt
     4621 tagcttttat tgcaggcgac catggcactg cttgtatttt ttaccagaac cgcacgggca
     4681 aggatcgtta cgtcctactt tgcgctcacc ggtttgcgcc gccagtgcag ctgcggctgc
     4741 agagtcgtca tcctgatggc taagctgctg catttgcgct aaacgctcgg cttccatacg
     4801 acgctgttgt tccagctcct caacctcttc aggcatacgt acctggactt tgctcagcgt
     4861 gctgataact tcatatttta acgactccag catcgctgca aacatggaga acgattcacg
     4921 tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac cctgacgcag
     4981 atagtccatc gccgccaggt gctctttcca cagagagtcg agagtttgca gcatgacgcc
     5041 tttttcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct gatacacttc
     5101 gatggactgc gccagaattc gctcacacag cgtctcttca tgcagttctg gttctttatc
     5161 cagccactcg gcaattggca aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc
     5221 cggaatatcc cacatttctt ccagtgactg tggcggaatg taggcatcaa tggtcgcttt
     5281 gaacacatct tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat cctgcagttc
     5341 gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat attccagcag
     5401 ttgcttacga atgtcgaagt tacggctttc aactttacgc tgggcgttgg caatcgcttt
     5461 agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac gcatcatgcc
     5521 ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca ggtagaaacg
     5581 ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat cgatacgacg
     5641 ggattcgtga cgctcggtac cgatgatatg caggccacct gcttccagta ccgcatcgtg
     5701 acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat tttccagcgc
     5761 ggcaacttct gcctgccagc taccaccgag cacaatatct gtaccacgac ccgccatatt
     5821 ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg cttcgttggc
     5881 gtggaatttg gcgttcagga cgttgtgctt aataccggct ttggtcagtt cgtttgacac
     5941 cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt tcgcagtacg
     6001 ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt agaccaggtc
     6061 cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat ccagcttgta
     6121 gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg ccagtttttc
     6181 atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt cgttctggat
     6241 ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc gacggccctg
     6301 catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa cgatgtagtc
     6361 gacgtcgcgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt gcatcagcat
     6421 gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt tcaccagcag
     6481 ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt tttcgtccac
     6541 cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg
     6601 cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg ggccggaaat
     6661 gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca ccagcgcata
     6721 gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atattgtcgc gcaggtagtc
     6781 aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt cgcgctttgc
     6841 cggtgctggc atgcccggca ggttgatacc gacagtcagg ccaaggaatt caaacagcgg
     6901 acggttgttt tcggcgtcac gttgcgccag gtagtcgttg acggtaacta cgtgcacgcc
     6961 tttaccggtt agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt taccttcacc
     7021 ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta actgaacgtc
     7081 gaagtgacgc ataccaaaga cgcgcttact tgcctcacgt accacggcga aagcttccgg
     7141 gatcagattt tccagcactt cgcctttttc cagacgtgca cgaaactctg cggttttccc
     7201 tttcagttct tcgtcggaga gtttttccat ctccggttcc atggcattga tgatgttgac
     7261 cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt tagttaacaa
     7321 tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa aatgaagtaa
     7381 aggtttattg ttgttaggtg aggcgttgag ggccagcacg gatgccttgc gcctggctta
     7441 tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca atgcgataac
     7501 ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa
     7561 gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagtgtttgc ggtgccattg
     7621 cgaaagaaag atgacggatt accgtgcgaa tggcatgttg atgccagtaa tcaacggaat
     7681 agttcgaatt cgggcggcgt gtgttcgctt ccagcaaggc caattgacca aagttaactt
     7741 tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cgcgtttggt tcggcggcgt
     7801 tgctgagcgc aggcaaacct aaactcgccg caaccatccc taataagaga tgcggccaga
     7861 agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta ttgccatccc
     7921 gatcatattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgca gtccacagca
     7981 cgaatgaaga taagttgtgc ggaaaagtgc ttaagaaaac agcgttcgca ccgtttttta
     8041 tagctcttat aaaattcaaa aaaaaacgac tggttcacct ggccggagag agtgccagat
     8101 ttgccagtcg aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc
     8161 tttgaaggcc aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg
     8221 tttcgccagg acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt
     8281 aaatgcacca ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt
     8341 gtgacgcaca aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc
     8401 aacaacgatg gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc
     8461 gatatcacgc atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc
     8521 ggagaagttc atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat
     8581 ggtgaaatcc agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc
     8641 gacacgaaca gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc
     8701 ggcgtcaagc agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg
     8761 cgcgttaact tcgataacaa tgttatcgat gcccaagccc gcgagagcag cattgaggtg
     8821 ctctacggtt gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc
     8881 acgcacagat ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata
     8941 gatgaccccg gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg
     9001 taaaccgaca cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt
     9061 atctcgccaa attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca
     9121 caaagagcct cgaaacccaa attccagtca attcttaatc agcttgctta cgcaggaatg
     9181 ctgggatatc cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcacgactt
     9241 tagcaaccgg cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca
     9301 tcactggctg ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc
     9361 cgatacctgt cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag
     9421 taccgataac cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt
     9481 cgaactcatc cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc
     9541 cagacaggtc gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg
     9601 cacggtcttc accgctcgcc acgccagaac ccatcattgc gtagcccatc tcagacatta
     9661 cggtgcgtac gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcagcga
     9721 taccttgcac agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga
     9781 taccgcggcc cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat
     9841 gcttggacag ttcagtgatc ccctgctccg cgaatgccat acgcttcttg ccttcaaagt
     9901 tgaaaggctt agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga
     9961 cgactggtgc tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt
    10021 ctgcaccttc cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa
    10081 cttctggatt agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct
    10141 gtccaaccgc tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac
    10201 cttcaatgcg ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga
    10261 tgactttaat caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat
    10321 ttgtgcctgt cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc
    10381 gcagccaact attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta
    10441 cttcagcttc accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg
    10501 aataatacgg ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac
    10561 gcacttgcgt atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc
    10621 caccggttaa tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt
    10681 cctgcaactg caatatctct tcgttgacca ggttgagcag ctcggtatag cgcggctcga
    10741 tcacctctgc cagggtctga cgttgcagac tccgcggcgg acgaccacct acgctcggca
    10801 cttccacgct ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa
    10861 tcgcttcggc gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat
    10921 tgccagcata aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat
    10981 ccattgtacc accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca
    11041 ataccgaata acttgatgcc agtccggcaa atatcagttg gtcaactttc agcccacaac
    11101 gttcaaccgc tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt
    11161 ttgcctgcat ccgcacgccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa
    11221 tcgcatactc ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt
    11281 tcgcggtatg gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca
    11341 ccataccaat ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag
    11401 agatctgaca atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca
    11461 cggattcgag gtcgttcacc ccgcctttat ccataccacg tgacgggcag ctgcccacgc
    11521 caatgatatt gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg
    11581 taccaatctc cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc
    11641 ctgtgcctga ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc
    11701 cgctccagag tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg
    11761 ctgctgtaaa accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg
    11821 gccaagattg agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat
    11881 cgccgcttcc ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc
    11941 ctgcaacact tcattggcgc tgccttccgg gccatacagc attggaagca cctgcttgct
    12001 ggtgcgatct ggcggcacgc tgaaggtgtt tccttccgcg tctaccatat gttgatcatt
    12061 ccaccgcgca atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt
    12121 tctgacgctc acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt
    12181 gacatcttgg gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc
    12241 gtcattacgc gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg
    12301 cgcatcttcc atccagccca acacgaccca gccgctcacc aacactgtcg ttaaaacggt
    12361 cagcaggaaa aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc
    12421 ttcgctgttt cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc
    12481 gtactaccaa ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt
    12541 ggctggtcat acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca
    12601 gcataacgtc aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta
    12661 attcctgcaa attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct
    12721 catcagagag atacttcgcc tcataatcat agaaggttcc ggacggttga atacgtattg
    12781 acggtaaaat ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt
    12841 caatcaatac ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt
    12901 ctgctactac ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg
    12961 gcaaacccag agcagaaatt tctgctaact gcttatcact caggcctttt tcaaactctg
    13021 cgcgggttaa cgcaacccac ggagcgaccg gtaaaccggc accttgccac agcagtttgc
    13081 tgcgtagttt atccattgaa agcgcagatg ccatcactcc actgccggta taaggcaagc
    13141 ccatcagctc gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga
    13201 taaacacttt ctgaaagccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga
    13261 caggatacgc atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca
    13321 gagaaacttc ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt
    13381 tgttcttcct ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt
    13441 ccgatattac cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc
    13501 atctcggcta cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga
    13561 attgtgcgac acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac
    13621 acttccagca tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg
    13681 tcgcgcgtac gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag
    13741 cctgcgcgcg ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc gtagtcatcg
    13801 accagcattg ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa
    13861 tcaaaacggc gaccagtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca
    13921 attccctctt ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacggcct
    13981 ggcgcattca gggtgacgcg catcggctct ttgtcctggc gcagcagcgt aaagtgcccc
    14041 tgcgggccaa tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa
    14101 gttgtggtct gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac
    14161 atcaccgcac gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt ctgttttaaa
    14221 ttctcaaagt cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc
    14281 accatcggtt gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac
    14341 cgaccatgcc ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag
    14401 gttgggtcga gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt tgtcgttttg
    14461 ccgtgcgttc ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg
    14521 gcacgacgga tcaccggaat acgcgcttca tgagctgcga caatttccgg gttatcggca
    14581 gaaatcgcgc tggaaacaac aaccacgctg gcatcacgta cgttttccgg gcgatggttg
    14641 aaataaatcg tcgctcccag attcattaac tgctgcgtga ccggatttgg cgctaaatcg
    14701 gaaccactga tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca
    14761 ccaccgatgc cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc
    14821 agttttgcca attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat
    14881 gcaaaagaca tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct
    14941 cggtggcatc cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc
    15001 gcgaccaccc ggccagggtg ttggcgacag catccacgct aagcagtggc tgctcgataa
    15061 ttttggctgc gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat
    15121 gttgaaacgg cacaaacaac gccggtagtc ctgccgcggc gatttcactc accgttaaag
    15181 caccggagcg gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa
    15241 attcagtcac tttatgctgc ggttgccccg cttcagcata cgcctgttca acggattgtt
    15301 gcgaaccttt gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct
    15361 gcggcattgt ctggttaaga atgcgtgcgc cctgggaacc acccactacc agcacacgaa
    15421 ccggaccttc acgtccagcc aaacgttgct gcggcaacgg tagcgccaac acatcggtac
    15481 gcaccgggtt acccaccact tccgcattgg ggaaagcacc tggaaacgcc tgcatcactt
    15541 tggtggcaat cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa
    15601 gtacaaccgg aatgcctaac gaccacgcgg ccagaccacc tggacctgat acatagccgc
    15661 ccataccgag taccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg
    15721 cgttgaagat acgcagcggc gcagctatca gtgcttttat accttttcca cgcagaccag
    15781 agatacgaat gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt
    15841 cggcagtccc cagccagcga acttgccaac cctgagccat tagatggtgc gcaaccgcca
    15901 gtcccgggaa tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac
    15961 cactcatcgt gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat
    16021 acgcaacagc atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat
    16081 cagcggcaat gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag
    16141 cgcctggaag ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc
    16201 aatttctaac gctttgcggc caatcgacat cgcgcgaaaa gcgacgaaga ataccattaa
    16261 aagtgccagc accacaccga cataccccag ttcttcgccg ataatggcga aaataaagtc
    16321 agtgtgcgct tccggcagat actccagttt ttgtaccgag ttacctaaac cttgtcccca
    16381 aagttcgccg cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg
    16441 atcttcccac gggttccaga atgcggtaac acggcggata cggtacggtt cggcgagtat
    16501 cagcaacaca accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc
    16561 tcccgccagg aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc
    16621 tggctgtgcc agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc
    16681 gcgcaggtta ttacgtactt cgtcgccttt acgcaccaga tagttggcga tataacaaaa
    16741 cagcgacagt tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg
    16801 cgatgcccct ttaaccgagc tacccactac cagcacgatc atcagcagga tgatagatcc
    16861 gagcagcatc gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat
    16921 cgccagaatg aatgccagga tcagatagac gccatcacgc ttcgcgaaga agaacggatc
    16981 gttggttaag cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc
    17041 cgcaaggccg aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc
    17101 tttttcccgc gagcccatca cccagccttt tagcgccgtg gagatccaga ccaggatact
    17161 gaatcctggc aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc
    17221 ttcgccagac gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg
    17281 gcacaggccg gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc
    17341 atcgcctgtt ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc
    17401 gcaccgtcac gaccaaaaca gtacaaacgc acgttgtcgc cattcagata gcgcgccagt
    17461 ggactgaagt ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacc
    17521 tgcaagccat tcaacgccgc ttccgtactg ccgacgttag tggctttcga atcgttaatc
    17581 cagcgtacgc cattgtgctc cagcaccact tcaaagcgat gcggcagacc ggtgaaggtg
    17641 gttaacgctt tcaggctgct ggcgcgcggc aaaccagctg cgtctgccag tgccagtgct
    17701 gccagcgcat tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact
    17761 ttctcgcctt taacccgcag ccaggtttcg ccctgctgat gattcaggtg atagtcaccc
    17821 atgttgacgc caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca
    17881 tcatcagcat taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga
    17941 tactgttgta aaccaaacgg atagcgatcc atatgatctt cagtcacgtt cagaatggtc
    18001 gctgctaccg cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg
    18061 tacagttcgc actcagcatc aagcagcatc aacgctggca ggccaatatt gccacccaca
    18121 ccaacgttaa cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg
    18181 ccgttagaac cggtaatcgc cacaatcggt gcttgtgctt cgcgacagaa cagctcgata
    18241 tcgccaacga tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca
    18301 ataccgggac tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc
    18361 gtgtggcgtt ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc
    18421 ataacgcgcg gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccagtgagg
    18481 cccaggccga taatgacgac atttttaccc tgataatcag ccatgattaa cgtaccttca
    18541 gcgttgccag accaatcaga accagcatca gcgaaataat ccagaaacgc acaatgacgc
    18601 gcggttccgg ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac
    18661 gttgtccgcg cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga
    18721 acacgccccc cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta
    18781 acgcaccacc taacgccagc gaacctacat cgcccataaa gacctgcgcc ggataggtgt
    18841 taaaccacag gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc
    18901 ctgcgtgtcg cagatacggt atatgcaagt agctggcaaa gttcatattg ccggtcgccc
    18961 acgccaccag cgcaaaacca ccggcgacaa atacggtcgg cataattgcc aggccgtcga
    19021 gaccatcggt caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa
    19081 tgtagaacag ccccagctgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg
    19141 cgggcgtgtc tttgccggca aggtacaggg cgaaggcgac acccagcgca atgaccgaca
    19201 tccagaaata cttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat
    19261 aatcatcaac aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt
    19321 acggattgga cgggtaagcc cacagcagta cggagatcac aatcgccgtc aggatcataa
    19381 tcccgcccat ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca
    19441 ccacctgacc aaaggaaagt ttttgcaaat gagcaatcat acgcgggccc atccacaatg
    19501 agatgaacag cgcggtcagc aagctgacga tggcgcgaaa cgtcagatag gaaaagacgt
    19561 taaagccgga ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc
    19621 ctgtaaagcg cgtactacct cttccatggc ggcactacgt gaacccttaa ctaaaatcgt
    19681 aattacctgt tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc
    19741 aaaatgttcg ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccacgcttaa
    19801 cacgcggtca ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct
    19861 ttcagcgccc agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc
    19921 cagtacctga acagcggcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag
    19981 caactggttt tctgccagtt ggatggggaa cagacggcct ggaacagctt tcagatttgc
    20041 cagccccgct ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc
    20101 cagcgcattc gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt
    20161 tggggtttgt agggtaaatt ccgtaccgtg cgaggtcaca tggatattgg tggcggtgaa
    20221 atcgctgttg gcggcattag gtgagaaacg ccacactttg cgtgagccaa ttacgctctg
    20281 ccagttcagc cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc
    20341 gctaaagatt tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg
    20401 cgccgctgcc aggttgttga ccagcgcagc ttccgggcga gtcagactca cagtccaggc
    20461 tatttcgccc tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa
    20521 gcgcaacaac gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt
    20581 gttgccgcac tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga
    20641 ccccgtcaga gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc
    20701 aaacgccaga cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct
    20761 aaccagtagt gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt gggcatcaaa
    20821 acgttcgcct ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt
    20881 tacagcatca agggtgatat ctgcaccttg cagttcaccg ttgagaatgt cggtaagttg
    20941 gctaagggtt acgctaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg
    21001 gagtagtcca gacgctgatt gccaacaatc tggtaatctt catggccttt gcccgcgacc
    21061 agtaccacat cattctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct
    21121 tccatcactt tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca
    21181 cgcggttctt cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg
    21241 gcaattgcgc ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg
    21301 caccacagct tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc cagtgcatcc
    21361 ggcgtatgcg cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa cacttccata
    21421 cgtccgcaaa ccggttgcag acgcgcggcg gttttcagca gatcagccag tggatagccg
    21481 agtgccagca gtgtcgccag cgcgagcagc aggttgctga cgttaaaagc gcccatcaga
    21541 tggctttcaa tttcgccatc gccccaactt gagctaaagc gaatcgtcgc accgctgtcg
    21601 tgatagttca cgtcgatcgc ttttaaccag cgtccgtgac agttcggatt gatatgatct
    21661 tccattgata ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg
    21721 tcggcgttaa taatcgcctg accgcaatga tgttcagaat aaagcagcca tttcgcagct
    21781 tcgtagtgtt ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc
    21841 gacgccgcaa atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc
    21901 attgcgcaaa acgtcgcgcc ctgctccacc agccccgcca gctcatgctg aacatcgact
    21961 gccgaaccgg tagtattttc tgtcgggatc actttcccaa gcagaccgtt accaacggtg
    22021 cccattaccg cgctggtttc gccaagcagt tggctccact gcgccagcag ctgggtagtc
    22081 gtggttttgc cgttggtgcc cgttacgcct acgagacgta aattgtcaga gggttcatgg
    22141 taaaagcggc ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt
    22201 acgccgtgca tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg
    22261 gcagccacac cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct
    22321 actacagcta caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc
    22381 agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct
    22441 gccacctgtc ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc
    22501 atccggctcg atgttcatgg tacgcaatac gccgcccatg atggcaccaa agaccggcgc
    22561 ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc
    22621 gaagcgcggc tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta
    22681 gcgaccgtcc ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt
    22741 aatcgccgct ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac
    22801 ggtgcgaaca atggattccg ggaagacacg ttcaccggga accggggggt caactttggt
    22861 aatcgacagt gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg
    22921 taacggtgtt accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca
    22981 ccgttgtttt tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt
    23041 tcccagtcca aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag
    23101 cttggaaaca ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta
    23161 gcgtgccacg tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga
    23221 gttttcccgc accacgccac gttgcaacgc ggtcattacc accatcggtt taaccgttga
    23281 gcccggttca aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct
    23341 cagattgtta gggttgtatg acgggctgtt agccatcgcc agcacttcac cggtgttgac
    23401 atccaccagc acggcgctac cggattcagc cttgttaaag gccaccgcgt tgttcagttc
    23461 gcgataaacc agcgcctgca agcgttcatc aatactcaac gccaggttgt gcgccgcctg
    23521 gctgtcagta gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg
    23581 ctcacccggc tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg
    23641 actatcgacg ttagtaaagc caatgaggtg agcagtcact tcgccggatg gataataacg
    23701 gcgagattct tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat
    23761 gtcagggttt acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg
    23821 ggctgaaagc tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc
    23881 gacgctgata ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg
    23941 cacgctcacc gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac
    24001 ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac
    24061 ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa
    24121 caacgcaaaa cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt
    24181 tttcgccgct gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg
    24241 tgacggatca acatgctgca tttgcagttt ttccgtggcg atcctttcca cccggctatg
    24301 gtcgccgagc gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg
    24361 ctccagcacc agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac
    24421 cacggtcacc gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg
    24481 caaaagatcg tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc
    24541 tttaactttg cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata
    24601 cgcagaactg aactacgggc acgaggattc tcagccacct cttcttcgcc cggcattaac
    24661 ttgcctaatg ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt
    24721 aaccctgccg gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg
    24781 tcttccagcg agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc
    24841 gagcttttta gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc
    24901 tggaaggtac gggtcgcggg atgtttgaat ttgtctttca ccggcgttgc ggcagccacg
    24961 acttccgcca gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg
    25021 cgggcaatgc gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg
    25081 gcttcttctg cggtttgcag ccattcagcg gctgactgcc cacgggttgg gtccatacgc
    25141 atgtccagcg gaccatcgcg cataaaggaa aagccgcgct cagcatcatc aagttgcggt
    25201 gaagagacgc caagatcgag gagaatgccg tcgatcttgc cgataagatc gcgctcggca
    25261 acgtattcgc ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata
    25321 gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg
    25381 ccaagctgcg agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag
    25441 atgccatcag gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt
    25501 ttatagtttt ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt
    25561 agccaactgc tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca
    25621 cagctcaaac ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg
    25681 ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg
    25741 acctaacagt aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa
    25801 tttttgctcg ataatttccc attcaggcag ggggtaaagc agcaagcacg ggtgatgaat
    25861 gtcaatggtg caaaccattt gaccggcagc gttctcaagc agctgttccc ggtaacgggt
    25921 aggcactgat aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc
    25981 agcctcaccc cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc
    26041 ggaggaaaag cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac
    26101 ggctaatatc agcgttgttt attttacgtc caacgtgcgc agcaaattaa cgttatgaat
    26161 agctgtaaga aaaaatccct catcgtcatc accgttttaa aatgactcac tattttccac
    26221 agcaaatata aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt
    26281 taacagtccc tgttgcgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag
    26341 aaaagtgctt gttaaatcgg caattcatct ctgcatgtaa caaaataatg caaaaacgcg
    26401 ctgtcgatat cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt
    26461 aaaagaactt atcaataata gtcaggaata gtcttattta ctttaaggat attgatgtcc
    26521 attcccgtac tctacgcgcc agagggaaat tcacctggcg cgtattattg ttcgcggctt
    26581 agctacggct gagcacaccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt
    26641 ttggcttacg cggttcgtcc aggcttgcca ggacaatctc cagcacacgc tctgcgacat
    26701 cgcggtgacg ttgagccacg gccagcaccg ggcactgtaa gaagtcgagc agttcgttat
    26761 cgccaaaggt ggcaattgca aggtcagaag gcagtttacc atcgcgacgt agcgtgacat
    26821 ccatcacccc ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg
    26881 tttccagcca tttttcgaat aactgggcag ccgcctcccg ctcatagctg ttggcataca
    26941 ggaaatgcac ttcgcgcgga tcatctttcc aggcagtacg gaaaccttgt tcacgcagga
    27001 agctgacaga aagctccggc agcgcgccaa gataaagcac cgtctcggcg ggaaacttac
    27061 gtaactcttc cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtgaaat
    27121 gttcacgatc gagggcgcgg tccagcgcga caatcgggaa cgggccgtta gcccagcgtt
    27181 gataaaaagg atgctcagga ggcaacgatg tcgaaacaat aatggcatcg acctgacgct
    27241 gtaaaaggtg ctcaatacag cgcatttcgt tgtctggctg atcttctgag caggcaatta
    27301 gcagttgata accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc
    27361 tggtgttctc cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc
    27421 cagctgccac ggcgttcggg tggtaattgt gctcacgcac cacagccatg actttttcaa
    27481 cggttttgtc gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg
    27541 tccgcgacac tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa
    27601 aatgtaaaaa ccatagccat tgtactggca tgggttaaat tcattaacat ctaagcgcag
    27661 aaaattaact gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc
    27721 aacgtgacat gttgggctgc aaattgcgca ttcagatcat tctcaacgca ttattttatc
    27781 gccgcgcgaa agtccgacca caccagaacg agcaacttcc acaattttcg ccacctcgcg
    27841 aatcgatgct aaaaatgcat caagcttatc gctggtgcct gctaattgaa cggtataaag
    27901 cgagggtgtg acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc
    27961 acgcccgtaa ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc
    28021 ctgccccaac tcgctcacgc gcaagacatc gaccagcttg tgtaattgct tttcgatctg
    28081 ctcaagcact ttttcatcgc ccacggtctg gatggtcata cgcgataatg tcggatcgtc
    28141 ggttggcgca acggtcaggc tttcaatgtt gtagccacgc tgggaaaaaa ggccaatcac
    28201 gcgggataac gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag
    28261 gttctctccg ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg
    28321 tagacgtgct cactgccatc gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc
    28381 tgttccagcg cctcgctaag tttgctttcc agctcatgcg gatgagagat ctggatcccg
    28441 acgtgaccat aggcttccgc cagacggacg aaatcgggta gtgattgcat ataagattgt
    28501 gaatgacggc cggaatagat catgtcctgc cactgtttca ccatccccag atagcggtta
    28561 ttgagattca ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctggatg
    28621 ttcatctgaa tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt
    28681 ttgacgccca gtgccgcagg taaaccaaaa cccatcgtgc cgaggccacc ggaattgatc
    28741 cagcgacgcg gtttgtcgaa tggataataa agtgcagcaa acatctggtg ctgcccgaca
    28801 tcggacgtca cgtaagcgtc cccattagtc aaccgccaaa gagtctcgat caccgcctgc
    28861 ggtttaatct tttcactgtg agtgtcatat ttcaggcact gacgagcgcg ccactgttca
    28921 atttgctgcc accagtcgcg gatctcatcc agtggttgat gggcggattc ttgcgacagg
    28981 agttcaagca tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc
    29041 acggttttag aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag
    29101 tactttgcca gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca
    29161 tccgcgttat gcatcgtcat attggcttcg taggtaccgt gcattcccag catgcccagt
    29221 gcctgacgat gcgttgccgg aaacgccccc agccccatca atgaggaaac aacgggcaga
    29281 ttcaacgcct ccaccgtttc tttcaactgc tgatggcagc ccgccgtgat tgccccaccg
    29341 cctacgtaga caaccggttt ttttgccgct accagcgttt gcagagcacg cttaatttgc
    29401 cctttatgtc cggtagtagt gggattgtaa gaacgcatac tgaccgactc aggccagaca
    29461 tagggtaatt tgttcgccgg attaagaata tctttcggta aatcaacgac cacgggtcct
    29521 gggcgaccac ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt
    29581 tgcttaacca gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc
    29641 tgaaaggcat cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga
    29701 atggaatcca tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc tggacccgac
    29761 gttaccagca cgacgccgac ttccccggtc gcacgcgcca ggccatcggc catatgcacc
    29821 gccgcctgct catgacgaac taatacatga tcaataccac ccacggtatg caatgcatcg
    29881 taaatatcaa ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata
    29941 agcgatcgga cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga
    30001 cggaaaaaat gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga
    30061 gacataaacg cagggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc
    30121 ctccattacg aaggattaaa ttgataataa atcggctgaa tcccacaact tacagcaatt
    30181 tctctatttt tttaattaat cctaaaatag agagtccaca cattcaggat aaaattctac
    30241 attcaataaa aaatgcagaa taaaccagac attcatgtct ggcctattct gcattcagtt
    30301 agcgtttgca aattgagact aattgttctt ccatccactg atggccttta tcgcgcccgg
    30361 cagcttcatg ccaggagaga taacaggttc tgctgttttg ttttaacggc agcggtaata
    30421 cctgtaattc taaggattca gcgaactctt cagccagcca acgcggcgca atagcgacca
    30481 aatgcgtttg cgacaccacg ctaagtacgc tcatcattgc catgccctga tacgcgatac
    30541 tggcttgctt atctaccgtg tcataccaag gttgactaaa tgacgcgaaa cgatcgagcg
    30601 aaaccgccgc atgttgttcg ttataaacat catgtttcag taacgggcct ttaattgttg
    30661 gatgattttt gctggctacc agcaccattt catctttaaa taatggcacg ctggtaaatt
    30721 caggacgatg gaagtcttca taactaatca caaactccgt ttcctgataa cgcagctgat
    30781 gttcagtgtt ctgatttaat gaagacttga acataacatg tatatttggc gcaatctgct
    30841 caatgtgatt ataaatctgc gaggtcagaa tgctgtctaa cgggctgcaa acacaaagat
    30901 gaaatacacg ttcactgctc gcgggttcaa aacctgaacc aggcaattca ttttgtacta
    30961 gttgcaatgc ctgacgaact gagccaaaaa gttgaaatgc gcgagcagtc ggttgaatac
    31021 cacggccata acgaacaaaa agctcgtcat taaacatcac cttcaggcgt gcaacagcgt
    31081 tactgaccgc aggttgcgac atacccagaa catgagcggc acgggtaatg ttttgctcct
    31141 gcatcacggc atcgaaaacg gttaataagt tgagatcgac catgcgtagc tgtggcttgc
    31201 ttaactccgc cgtctctgaa tgatctgttt gtacctctgg catacttaac tccactgtca
    31261 cacttaactc cctttccctt attggaatgc agagaaataa tcctgaagaa tatgatttat
    31321 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
    31381 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
    31441 ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac
    31501 aaaatggatt aaatgtgctt atttaataat t
//
LOCUS       DAZFVO020000042        31409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFVO020000042 DAZFVO020000000
VERSION     DAZFVO020000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.042"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..567
                     /locus_tag="ACHKNB_RS09095"
                     /old_locus_tag="pgaptmp_001819"
     CDS             <1..567
                     /locus_tag="ACHKNB_RS09095"
                     /old_locus_tag="pgaptmp_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HGU4195348.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            797..1180
                     /gene="secE"
                     /locus_tag="ACHKNB_RS09100"
                     /old_locus_tag="pgaptmp_001820"
     CDS             797..1180
                     /gene="secE"
                     /locus_tag="ACHKNB_RS09100"
                     /old_locus_tag="pgaptmp_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGU4195349.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1182..1727
                     /gene="nusG"
                     /locus_tag="ACHKNB_RS09105"
                     /old_locus_tag="pgaptmp_001821"
     CDS             1182..1727
                     /gene="nusG"
                     /locus_tag="ACHKNB_RS09105"
                     /old_locus_tag="pgaptmp_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGU4195350.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1886..2314
                     /gene="rplK"
                     /locus_tag="ACHKNB_RS09110"
                     /old_locus_tag="pgaptmp_001822"
     CDS             1886..2314
                     /gene="rplK"
                     /locus_tag="ACHKNB_RS09110"
                     /old_locus_tag="pgaptmp_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGU4195351.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2318..3022
                     /gene="rplA"
                     /locus_tag="ACHKNB_RS09115"
                     /old_locus_tag="pgaptmp_001823"
     CDS             2318..3022
                     /gene="rplA"
                     /locus_tag="ACHKNB_RS09115"
                     /old_locus_tag="pgaptmp_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGU4195352.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHKNB_RS09120"
                     /old_locus_tag="pgaptmp_001824"
     CDS             3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHKNB_RS09120"
                     /old_locus_tag="pgaptmp_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGU4195353.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3999..4364
                     /gene="rplL"
                     /locus_tag="ACHKNB_RS09125"
                     /old_locus_tag="pgaptmp_001825"
     CDS             3999..4364
                     /gene="rplL"
                     /locus_tag="ACHKNB_RS09125"
                     /old_locus_tag="pgaptmp_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGU4195354.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHKNB_RS09130"
                     /old_locus_tag="pgaptmp_001826"
     CDS             4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHKNB_RS09130"
                     /old_locus_tag="pgaptmp_001826"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGU4195355.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHKNB_RS09135"
                     /old_locus_tag="pgaptmp_001827"
     CDS             8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHKNB_RS09135"
                     /old_locus_tag="pgaptmp_001827"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGU4195356.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHKNB_RS09140"
                     /old_locus_tag="pgaptmp_001828"
     CDS             13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHKNB_RS09140"
                     /old_locus_tag="pgaptmp_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HGU4195357.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHKNB_RS09145"
                     /old_locus_tag="pgaptmp_001829"
     CDS             complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHKNB_RS09145"
                     /old_locus_tag="pgaptmp_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HGU4195358.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHKNB_RS09150"
                     /old_locus_tag="pgaptmp_001830"
     CDS             complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHKNB_RS09150"
                     /old_locus_tag="pgaptmp_001830"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HGU4195359.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHKNB_RS09155"
                     /old_locus_tag="pgaptmp_001831"
     CDS             complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHKNB_RS09155"
                     /old_locus_tag="pgaptmp_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HGU4195360.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHKNB_RS09160"
                     /old_locus_tag="pgaptmp_001832"
     CDS             complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHKNB_RS09160"
                     /old_locus_tag="pgaptmp_001832"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HGU4195361.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHKNB_RS09165"
                     /old_locus_tag="pgaptmp_001833"
     CDS             complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHKNB_RS09165"
                     /old_locus_tag="pgaptmp_001833"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGU4195362.1"
                     /translation="MYQPDFPLVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHKNB_RS09170"
                     /old_locus_tag="pgaptmp_001834"
     CDS             complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHKNB_RS09170"
                     /old_locus_tag="pgaptmp_001834"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HGU4195363.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19619..19719)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHKNB_RS09175"
                     /old_locus_tag="pgaptmp_001835"
     CDS             complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHKNB_RS09175"
                     /old_locus_tag="pgaptmp_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HGU4195364.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20342..21115
                     /gene="nudC"
                     /locus_tag="ACHKNB_RS09180"
                     /old_locus_tag="pgaptmp_001836"
     CDS             20342..21115
                     /gene="nudC"
                     /locus_tag="ACHKNB_RS09180"
                     /old_locus_tag="pgaptmp_001836"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HGU4195365.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21155..22219
                     /gene="hemE"
                     /locus_tag="ACHKNB_RS09185"
                     /old_locus_tag="pgaptmp_001837"
     CDS             21155..22219
                     /gene="hemE"
                     /locus_tag="ACHKNB_RS09185"
                     /old_locus_tag="pgaptmp_001837"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HGU4195366.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22229..22900
                     /gene="nfi"
                     /locus_tag="ACHKNB_RS09190"
                     /old_locus_tag="pgaptmp_001838"
     CDS             22229..22900
                     /gene="nfi"
                     /locus_tag="ACHKNB_RS09190"
                     /old_locus_tag="pgaptmp_001838"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HGU4195367.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHKNB_RS09195"
                     /old_locus_tag="pgaptmp_001839"
     CDS             22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHKNB_RS09195"
                     /old_locus_tag="pgaptmp_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HGU4195368.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23720..23992
                     /gene="hupA"
                     /locus_tag="ACHKNB_RS09200"
                     /old_locus_tag="pgaptmp_001840"
     CDS             23720..23992
                     /gene="hupA"
                     /locus_tag="ACHKNB_RS09200"
                     /old_locus_tag="pgaptmp_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HGU4195369.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHKNB_RS09205"
                     /old_locus_tag="pgaptmp_001841"
     CDS             24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHKNB_RS09205"
                     /old_locus_tag="pgaptmp_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HGU4195370.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24702..25127)
                     /gene="zraP"
                     /locus_tag="ACHKNB_RS09210"
                     /old_locus_tag="pgaptmp_001842"
     CDS             complement(24702..25127)
                     /gene="zraP"
                     /locus_tag="ACHKNB_RS09210"
                     /old_locus_tag="pgaptmp_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HGU4195371.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25365..26741
                     /gene="zraS"
                     /locus_tag="ACHKNB_RS09215"
                     /old_locus_tag="pgaptmp_001843"
     CDS             25365..26741
                     /gene="zraS"
                     /locus_tag="ACHKNB_RS09215"
                     /old_locus_tag="pgaptmp_001843"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HGU4195372.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26738..28063
                     /gene="zraR"
                     /locus_tag="ACHKNB_RS09220"
                     /old_locus_tag="pgaptmp_001844"
     CDS             26738..28063
                     /gene="zraR"
                     /locus_tag="ACHKNB_RS09220"
                     /old_locus_tag="pgaptmp_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HGU4195373.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28060..29349)
                     /gene="purD"
                     /locus_tag="ACHKNB_RS09225"
                     /old_locus_tag="pgaptmp_001845"
     CDS             complement(28060..29349)
                     /gene="purD"
                     /locus_tag="ACHKNB_RS09225"
                     /old_locus_tag="pgaptmp_001845"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGU4195374.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTNIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29361..30950)
                     /gene="purH"
                     /locus_tag="ACHKNB_RS09230"
                     /old_locus_tag="pgaptmp_001846"
     CDS             complement(29361..30950)
                     /gene="purH"
                     /locus_tag="ACHKNB_RS09230"
                     /old_locus_tag="pgaptmp_001846"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGU4195375.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7408 a         8134 c         8599 g         7268 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggtcgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      541 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
      601 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      661 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      721 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaaaact
      781 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
      841 agcgatgaag tgggtcgttg tggtggcatt gctcctggtg gcgattgtcg gcaactacct
      901 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
      961 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc gttgcttttg cccgtgaagc
     1021 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acaccacgct
     1081 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
     1141 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
     1201 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgtcgctgc
     1261 gtgagcatat caaattacac aacatggaag atttgtttgg tgaagtcatg gtaccaaccg
     1321 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
     1381 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacctggtg cgcagcgtac
     1441 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
     1501 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgc
     1561 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggtg
     1621 ttgttgaaga agtggattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1681 gtgcgacccc ggtagagctg gacttcagcc aggttgaaaa agcctaaccc agcgatcaaa
     1741 aaagcggcga tttaatcgtt gcacaaggcg tgagattgga atacaatttc gcgccttttg
     1801 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     1861 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     1921 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     1981 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     2041 tctgccgatt ccggtagtaa tcaccgttta cgctgaccgt tctttcactt tcgttaccaa
     2101 gactccgccg gcagcagttc tgctgaaaaa agcggctggt atcaagtctg gttccggtaa
     2161 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     2221 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     2281 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     2341 gcgtgttatc cgcgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     2401 actgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     2461 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     2521 tactggccgt tccgttcgcg tagccgtatt tacccaaggt gcaaacgctg aagctgctaa
     2581 agctgcaggc gcagaactgg taggtatgga agatctggct gaccagatca agaaaggcga
     2641 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2701 ccaggttctg ggtccgcgcg gcctgatgcc aaacccgaaa gtgggtactg taacaccgaa
     2761 cgttgctgaa gcggttaaaa acgctaaagc tggccaggtt cgttaccgta acgacaaaaa
     2821 cggcatcatc cacaccacca tcggtaaagt ggactttgac gctgacaaac tgaaagaaaa
     2881 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     2941 catcaagaaa gttagcatct ccaccaccat gggtgcaggt gttgcagttg accaggctgg
     3001 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     3061 ccccacgtat aatgcttaat gcagacgtat atccgagata ttcgggttgt ggcaaggcgg
     3121 caactgagtg agtcgccagg agcatagcta actatgtgac tggtgcgaat gaaggaagcc
     3181 aacgccgcca caagttgaat agcgacggat agaaaagatt tgttcgttgg agcctggcct
     3241 atccaggcct ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac
     3301 ggtgacagaa cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta
     3361 agacgctctc tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc
     3421 caggagcaaa gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca
     3481 gcgaagtagc caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag
     3541 ataaaatgac tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc
     3601 gtaacaccct gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt
     3661 ttgttggtcc gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt
     3721 tcaaagagtt cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg
     3781 agctgatccc ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa
     3841 ttgcacgcct gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg
     3901 ctgctgtacg cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcattcgct
     3961 tacgtataaa cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa
     4021 tcattgaagc agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg
     4081 aagaaaaatt cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag
     4141 ctgctgaaga aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg
     4201 ctgttatcaa agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg
     4261 tagaatctgc accggctgca ctgaaagaag gcgtgagcaa agacgacgca gaagcactga
     4321 aaaaagctct ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt
     4381 gcagcctgag aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg
     4441 taaggcgcca gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc
     4501 tatcgacgac ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg
     4561 tttaagcgtg atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag
     4621 tgttgcacaa actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac
     4681 cctatggttt actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca
     4741 caagttctgg atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc
     4801 gagcaagatc ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt
     4861 cagagctaca gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg
     4921 tttgacgtcc aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa
     4981 ctgcgtctgg tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa
     5041 caagaagtct acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac
     5101 ggtactgagc gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc
     5161 gacaaaggta aaacccactc ttcgggtaaa gtgctgtata acgcgcgtat catcccttac
     5221 cgtggttcct ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac
     5281 cgtcgccgta aactgcctgc gaccatcatt ctgcgcgccc tgaactacac cacagagcag
     5341 atcctcgacc tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg
     5401 gaactggtgc cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt
     5461 aaagtgtacg tagaaaaagg ccgccgtatc actgcgcgcc acattcgcca gctggaaaaa
     5521 gacgacgtca aactgatcga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa
     5581 gactatattg atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg
     5641 gatctgctgg ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac
     5701 gatctggatc acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt
     5761 ctgagcgcac tggtagaaat ctaccgcatg atgcgccctg gcgagccgcc gactcgtgaa
     5821 gcagctgaaa gcctgttcga gaacctgttc ttctccgaag accgttatga cttgtctgcg
     5881 gttggtcgta tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatc
     5941 ctgagcaaag acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa
     6001 ggcgaagtcg atgatatcga ccacctcggc aaccgtcgta tccgttccgt tggcgaaatg
     6061 gcggaaaacc agttccgcgt tggcctggta cgtgtagagc gtgcggtgaa agagcgtctg
     6121 tctctgggcg atctggatac cctgatgcct caggatatga tcaacgccaa gccgatttcc
     6181 gcagcagtga aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac
     6241 ccgctgtctg agattacgca caaacgtcgt atctccgcac tcggcccagg cggtctgacc
     6301 cgtgaacgtg caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt
     6361 ccaatcgaaa cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca
     6421 cagactaacg aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta
     6481 actgacgaaa ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg
     6541 aactccaacc tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa
     6601 ggcgaatcca gcttgttcag ccgcgaccag gttgactaca tggacgtatc cacccagcag
     6661 gtggtatccg tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca
     6721 ttgatgggtg cgaacatgca acgtcaggcc gttccgactc tgcgcgctga taagccgctg
     6781 gttggtactg gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggtagctaaa
     6841 cgtggtggtg tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac
     6901 gagatgtatc cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct
     6961 aaccagaaca cctgtatcaa ccagatgccg tgtgtgtctc tgggtgaacc ggttgaacgt
     7021 ggcgacgtgc tggcagacgg tccgtccacc gacctcggtg aactggcgct tggtcagaac
     7081 atgcgcgtag cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc
     7141 gagcgtgttg ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg
     7201 tcccgtgaca ccaagctggg gccggaagag atcaccgctg acatcccgaa cgtgggtgaa
     7261 gctgcgctct ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt
     7321 ggcgacattc tggttggtaa ggtaacgccg aaaggtgaaa ctcagctgac cccagaagaa
     7381 aaactgctgc gtgcgatctt cggtgagaaa gcctctgacg ttaaagactc ttctctgcgc
     7441 gtaccaaacg gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta
     7501 gaaaaagaca aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac
     7561 ctgtctgaag aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg
     7621 gtagccggtg gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag
     7681 ctgggcctga cagacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac
     7741 gaactgaaac acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc
     7801 gacgatctgg caccgggcgt gctgaagatt gttaaggtat atctggcggt taaacgccgt
     7861 atccagcctg gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc
     7921 aacccgatcg aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac
     7981 ccgctgggcg taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg
     8041 gctgcgaaag gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg
     8101 aaactgcgcg aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt
     8161 gacctgagta ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt
     8221 atgccaatcg caacgccggt gttcgacggt gcgaaagaag cagaaattaa agagctgctg
     8281 aaacttggcg acctgccgac ttccggtcag atccgcctgt acgatggtcg cactggtgaa
     8341 cagttcgagc gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc
     8401 gacgacaaga tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg
     8461 ggtggtaagg cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa
     8521 gcatacggcg cagcatacac cctgcaggaa atgctcaccg ttaagtctga tgacgtgaac
     8581 ggtcgtacca agatgtataa aaacatcgtg gacggcaacc atcagatgga gccgggcatg
     8641 ccagaatcct tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg
     8701 gaagacgagt aattctcgct caaacaggtc actgctgtcg ggttaaaacc cggcagcgga
     8761 ttgtgctaac tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca
     8821 gactaaaacc gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg
     8881 ttcatggtct ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc
     8941 agaacgtgac ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct
     9001 gtgcggtaag tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggcgttga
     9061 agtgacccag actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac
     9121 tgcgcacatc tggttcctga aatcgctgcc gtcccgtatc ggtctgctgc tcgatatgcc
     9181 gctgcgcgat atcgaacgcg tactgtactt tgaatcctat gtggttatcg aaggcggtat
     9241 gaccaacctg gaacgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga
     9301 gttcggtgac gaattcgacg cgaagatggg ggcggaagca atccaggctc tgctgaagag
     9361 catggatctg gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga
     9421 aaccaagcgt aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg
     9481 taacaaacca gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc
     9541 gctggttccg ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg
     9601 cgtcattaac cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat
     9661 cgtacgtaac gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg
     9721 tcgcggtcgt gcgatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat
     9781 caaaggtaaa cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg
     9841 tcgttctgta atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa
     9901 aatggcgctg gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc
     9961 taccaccatt aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat
    10021 cctggacgaa gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg
    10081 tctgggtatc caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc
    10141 gctggtttgt gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc
    10201 gctgacgctg gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct
    10261 gtccccggcg aacggcgaac caatcatcgt tccgtctcag gacgttgttc tgggtctgta
    10321 ctatatgacc cgtgactgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa
    10381 agaagcagaa cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg
    10441 tatcaccgag tatgaaaaag atgcaaacgg tgaattagta gcgaaaacca gcctgaaaga
    10501 cacgactgtt ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttactccat
    10561 cgtcaaccag gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat
    10621 tctcggtctg aaaccgaccg ttatttttgc ggaccagatc atgtacaccg gcttcgccta
    10681 tgcagcgcgt tctggtgcat ctgttggtat cgatgacatg gtcatcccgg agaagaaaca
    10741 cgaaatcatc tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg
    10801 tctggtaact gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga
    10861 tcgtgtatcc aaagcgatga tggataacct gcaaactgaa accgtgatta accgtgacgg
    10921 tcaggaagag aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg
    10981 tggttctgcg gcacagattc gtcagcttgc tggtatgcgt ggtctgatgg cgaagccgga
    11041 tggctccatc atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca
    11101 gtacttcatc tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaactgc
    11161 gaactccggt tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga
    11221 agacgattgt ggtacccatg aaggtatcat gatgactccg gttatcgagg gtggtgacgt
    11281 taaagagccg ctgcgcgatc gcgtactggg tcgtgtaact gctgaagacg ttctgaagcc
    11341 gggtactgct gatatcctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct
    11401 gctggaagag aactctgtcg acgcggttaa agtacgttct gttgtatctt gtgacaccga
    11461 ctttggtgta tgtgcgcact gctacggtcg tgacctggcg cgtggccaca tcatcaacaa
    11521 gggtgaagca atcggtgtta tcgcggcaca gtccatcggt gaaccgggta cacagctgac
    11581 catgcgtacg ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca
    11641 ggtgaaaaac aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg
    11701 taaactggtt atcacttccc gtaatactga actgaaactg atcgacgaat tcggtcgtac
    11761 caaagaaagc tacaaagtac cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt
    11821 tgctggcggc gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt
    11881 aagcggtttt gtacgcttta ctgacatgat cgacggtcag accattactc gtcagacaga
    11941 cgaattgacc ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg
    12001 taaagatctg cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc
    12061 aggtaccgat atgcctgcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga
    12121 tggcgtacag atcagctctg gtgacaccct ggcgcgtatt ccgcaggaat ccggcggtac
    12181 caaggacatc accggtggtc tgccgcgcgt tgcggacctg ttcgaagctc gtcgtccgaa
    12241 agagccggca atcctggctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg
    12301 taaacgtcgt ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc
    12361 gaaatggcgt cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc
    12421 cgacggtccg gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgtgactcg
    12481 ttacatcgtt aacgaagtac aggacgtata ccgtctgcag ggcgttaaga ttaacgataa
    12541 acacatcgaa gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggcag
    12601 ctccgacttc ctggaaggcg aacaggttga atactctcgc gtcaagatcg caaaccgcga
    12661 actggaagcg aacggcaaag tgggtgcaac ttactcccgc gatctgctgg gtatcaccaa
    12721 agcgtctctg gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt
    12781 gctgaccgaa gcagccgttg cgggcaaacg cgacgaactg cgcggcctga aagagaacgt
    12841 tatcgtgggt cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg
    12901 tcgccgtgct gcgggtgaag ctccggctgc accgcaggtg actgcagaag acgcatctgc
    12961 cagcctggca gaactgctga acgcaggtct gggcggttct gataacgagt aatcgttaat
    13021 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag
    13081 agcatttgtc agaatattta aggaatttct gaatactcat aatcaatgta gagatgacta
    13141 atatcctgaa actgactgaa ctaattgagt caaactcggc aaggattcga tactattcct
    13201 gtgtaacttt cttaaggaac gagaatgaaa caggaagtgg aaaagtggcg accttttgga
    13261 catccggatg gtgatattcg tgatttatca tttcttgatg ctcatcaggc tgtctacgtt
    13321 cagcatcatg agggcaaaga gcctttagag tatcgctttt gggttaccta ctctcttcac
    13381 tgcttcacaa aagattatga acatcagacg aacgaagaaa aacaatcgtt aatgtaccac
    13441 gcgcctaaag aatctcgtcc cttctgccag caccgttata acttagcgcg cacacactta
    13501 aaaagaacta ttttggcgct gccagaaagc aacgttattc atgccgggta tggtagctat
    13561 gccgtgattg aggtggactt agacggagga gataaggcat tttactttgt tgcgttcagg
    13621 gctttcaggg aaaagaaaaa actccgtttg catgtaacta gcgcttatcc catttctgaa
    13681 aaacagaaag gtaaatcagt gaaatttttc accattgcct acaacttatt gagaaataag
    13741 cagcttcctc agccctcaaa ataacaaaac ccaccttaag gtgggtttcg ccagagaatt
    13801 atctctggta ttcagaacgc cattaccgga ctttgccttg accttgcgat aatcgcaggt
    13861 tgcgggatgt ctgaatttct tcagtctgct gcatcctgga agatgagaac atgtgttctt
    13921 attttcgtct ctatcatagt tgagtattta ctctcttaca atcagatctc tttcattgct
    13981 caacaggcga tggcttcaga ctttgcatta cggaattttt aagaaaggca gggcgaaacg
    14041 aggaagaagc ttttcgccct ggacaacatt cctgctgacg gcactaccat aaaaaaacag
    14101 aacgctttca acaaccttgc gtttttcatc ttccgtagcg gctcggaata aatttacgtt
    14161 cttacattcc gtctcatagt ctttgcgagg cccgtcccag atagctgtcc cagtctttcc
    14221 ataccggctg caaaccctga gcggttaacg cggcagcaac cgcttccggt ctgcgatcgt
    14281 cgtgcggtga gaactgttcc aactcggggt gattatcggc atagccacct ggctgcgttt
    14341 tcgagaaggc gctgacgtta ttgatcgcca gcggaataac gcgatcgcga aaccacggtg
    14401 attcccgcgt ggagagtgac agttcaatct ccggtgcaag cagtcggaag gcgcagatgg
    14461 tttgcactaa ctggcgttca tccataatcg acgcaggctc aatgccgcca gtacacgggc
    14521 gcaggcgcgg aaaggagaca gagtaacggc tttgccagta atgctgttgc agccatagca
    14581 aatgttcggc aaccatatag cagtcaacgc gccagttgtc ggaaaggcca attagcgcgc
    14641 cgaggcctat cttatcaatc cccgcacgcc ccagccgatc cggcgtttcc agccgccaga
    14701 agaagtcctg tttttttcct ttcagatgat ggcgggcata agtcgcctcg tgatatgtct
    14761 cctgataaac catcacgcca tccagaccaa gctgctttaa ctcggcgtat tccgtctccg
    14821 ccagcggttg cacttccatt tgtagtgaag agaactgttc acgcagggcg gggagatgac
    14881 gacgaaagta atccatcccc actttcgcct gatgttcacc agtgactaat agcagatgtt
    14941 caaagcccat ctcccgtata gcggcgcttt ccctggcaat atccgcttca tccagcgttt
    15001 tgcgcttgat gcgattactc atggaaaatc cgcagtacgt gcagtcgtta gcgcaaagat
    15061 tggaaagata aagcgggacg tagaaactaa ctgtgttgcc aaatcgctga cgggtcagac
    15121 gctgcgcccg ttgggccagt tgttccagat agccactggc ggctggcgat aacagcgcca
    15181 tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg ttctacgtca gcagccgttt
    15241 tgccgttgat acgcaggcgg atgtcgtccc agtccagttg tcgccagcga tcgctgaagg
    15301 ttttcatgcc gatgcctcca gaaatccagt cagcgggctg gtggcatgag caaaatgact
    15361 gcggctgccc ggtccggact gacgtgccag caggcctgct tctaccgcca gacgaaatgc
    15421 cttcgccatg ttgacgggat cgtccgcgac ggcaattgcc gtattcacta acaccgcgtc
    15481 ggcccccatt tccagcgcct gcgcggcatg gctgggaacg ccgatgccag catcgacaac
    15541 caccggcact gtggcctgct ggataataat ctccagcatg gcgcgggttt ccagtccctg
    15601 attcgagcca atcggcgcgc cgagcggcat caccgctgca cagccgactt cttccagacg
    15661 tttacacaat accggatcgg ccccgcagta aggcagcacg acaaatccct gttgtaccag
    15721 cgtttcggcg gctttcaggg tttcgatggg atcgggcaac agccagcggg cgtcagggtg
    15781 aatctctaat tttaaccagt ttgtgcctaa cgcttcacga gccagatggg cggcgaaaat
    15841 ggcttcttcc gctgttttcg ccccggatgt atttggcagc agggtcacac ccgccgcgat
    15901 aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg acacgtttca tcgccagtgt
    15961 caccagctgg ctgccggaag cgcggatcgc ctccaccatc agttgtgaag aagcgaattt
    16021 ccctgtgccg gtaaacagat gtgaatcaaa cgttttgtcc gcaatacgta acatttcaac
    16081 cccctgcaat aacctgaaaa agcaggatct ggtcgccatc ctgcacgata tgttgcgccc
    16141 actgctcacg cgggacgatt tgctgattaa tcgccagagc cgcgcccgct tgtcgttggt
    16201 cgagttgctc cagtagttcg tgaacagttt gcccggcggc gcactgcatc ggttgatcgt
    16261 taaacaggat ctgcattgct tcctccgcat accgggcaac cactggcgcg gcgcaacgcc
    16321 aggctgcgcc actggctcga tttaccgtcg aacagtcgga gttctcccgc aggtgtctct
    16381 ataccgctta ataacttaat ggcttccagt gcctgcaaag tgcccataac cccgaccacc
    16441 gggccaacca cgcccgccgt gcggcagttg cgttctggct cctggttatc tggccacagg
    16501 cagcggtaac acccctgctc ccagggcggc gtcagtacca tcaactgacc gccaaatccg
    16561 accgcgctgg cggtgataag cggcgtgttg agtgccacgc aggcggcatt aatctcctgg
    16621 cgagtcgcca tattgtcggt acagtcgagc accacatcgg cccgtgcaac cgcatctttt
    16681 aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt gaatgtcggg attcaactgt
    16741 gtcagtcgct gttggctgac ctgcgatttc gggcgatcga tatcttcagt ggtaaagagg
    16801 atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat ctgccagtac cagcgtcccg
    16861 acgccagcgc ccgccaggta cagcgcagca ggtgtaccca gcccgcccag accgataatc
    16921 agcacctggc tgtcgagcag tttttgctgc ccgtccagag cgatatcgtc gagcaggatt
    16981 tggcggctat aacgcataaa gtcacggtca ttcatcgcca actcctgcaa tttccagcaa
    17041 ctgtgccgtt gccaaacgcc agtctgcagc ttgagtaatg gcgctgacga cggcgatact
    17101 gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga ctgataccgc caatcgccac
    17161 ggtgggataa tccgccagtc gctcaacatg ccgtgccagc tgttccagcc cctgcggtgc
    17221 agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc agcgcgatat aagaggggcg
    17281 tgctgccagc gcgacgtcga tttccatatc gtcatgtgtc gaaacgccca gccgcaggcc
    17341 tgccgcgcgg atggcattga gatcggtggc ttgcaaatct tcctgcccca aatggacgcc
    17401 atacgcctga tgcttgatcg ccagccgcca gtaatcgttg ataaacaatc gcgcgttata
    17461 gcggcggccc agcgcaattg ccgccacgac atcggcttcc acctcttcat cgcgccgatc
    17521 tttgatgcgt agctggagag tacgtacgcc tgcatccaac agacgttcga tccactgtac
    17581 gctgtccacc accgggtaca gtcctgaacg aaaaggtaca agaggaaaat caggctgata
    17641 catcacgctt cctccttacg caggtagatt tctccgcctc tggcacggaa gttctccgac
    17701 atatccgcca ttcccatttc aatagtttgc gtggcggcgt aatcacgcac ttcctggctg
    17761 attttcatcg agcagaattt cggcccacac atggagcaaa aatgggcgac tttacctgac
    17821 tcttgcggca gggtttcatc gtgataagcg cgggcggtaa acgggtcgag ggccagatta
    17881 aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca tggcgttatc gcgaatttgc
    17941 gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg caatcttata ggtgataagc
    18001 ccctgcttaa catcttcttt attgggcaga cccagatgct cttttggcgt tacgtaacag
    18061 agcatcgcgc agccaaacca gccaatcatc gccgcaccaa tccccgacgt gaagtggtca
    18121 tagcccggcg caatatcggt agttagcggc cccagagtgt aaaacggcgc ttcgtggcag
    18181 tgctctaact cctcggtcat attgcggcgg atcatctgca tcggcacgtg gcctgggcct
    18241 tcaatcatca cctgcacgtc atattcccag gcaattttgg tcagttcgcc cagcgtatgc
    18301 agctcggcaa actgcgcttc atcgttggcg tcctgaatag aaccggggcg cagaccgtcg
    18361 cccagcgaca gcgaaacgtc ataagcggca cagatttcac aaatttcgcg gaagtgttga
    18421 tagaggaaat tttcctgatg atgggagagg caccatttcg ccataatcga accgccgcga
    18481 gagacgatac cggtcaggcg tttcgcggtc atcggcacat agcgcagcag tacgcccgca
    18541 tggatagtga agtaatccac accttgctcg gcctgttcca gcagcgtgtc gcggaacgct
    18601 tcccaggtaa gatcttcggc gatcccgtta accttctcca gcgcctggta gatcggcact
    18661 gtaccgatcg gcaccgggct gttacgcaaa atccactcgc gggtttcgtg aatatagcga
    18721 ccggtggaga gatccatcac cgtatccgct ccccagcgcg tggaccatac cagcttttcc
    18781 acttcttctt cgatggaaga ggtgaccgcc gagttgccga tattggcgtt aacttttacc
    18841 aggaaattgc gaccaataat catcggctcc gattccggat gattaatgtt ggccgggata
    18901 atcgcacgtc cggcagcaac ttcatcacgg acaaattccg cagtgatatt ttccggcaga
    18961 cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa cttcgctacg gatgcgctcg
    19021 cggcccatat tctcgcggat ggcgatgaat tccatttccg gcgtgatgat gccctggcgg
    19081 gcgtagtgca gttgggtgac acggcgtcct gctttggcgc gttttggtgt tagtacgccg
    19141 ctaaaacgca gttcgtcgag gccatcatct gccagccgcg ctttagtgta atcggaactg
    19201 cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc acggctggcg tagttttgcc
    19261 agcccttgct gcacgttaat ggcaatctgc ggatcaccat acgggccgga ggtgtcgtag
    19321 accggaatcg cttcgttttc ttcgtactgc ggctgttctt tgctaccgcc aattagcgtc
    19381 gggctaagct ggatctcacg catcggcacg cgcacgccgg ggtgtgtgcc agtgatataa
    19441 atgcgttttg agttgggaaa ggcggtgcct tccagggtgt cgataaaatg ttgggcccgg
    19501 gcgcgttgtt cgcggcgggt cagttttgtt gcagacatag ctcattccaa aaagttaagg
    19561 acgtggcttg tcagacgacg gatgaagcaa gagacgatcg ccgcaggggc gatgcgatag
    19621 cagattaact cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat
    19681 aaacggtctc agccagttaa ggcactccga caagaaatta gccccgcaaa tggggcattg
    19741 aatgtaaatt acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc
    19801 aagcaccagc agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat
    19861 gtcagataaa acctgttgaa actcaaggta attatcatga tcgatggcgg tttccagact
    19921 ggaatcgtag taatccataa tctgttgagt attggcttcg agttgcggcc aaatcttcgc
    19981 ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa
    20041 atgtccggca gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt
    20101 tagcctcatg tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag
    20161 cagatgctta cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc
    20221 cgtcaggtta tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc
    20281 tcagtgagaa atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc
    20341 tatggatcgt ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa
    20401 attatggttg ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg
    20461 tcagcgcgca ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca
    20521 gcggcgtcac gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca
    20581 actggccgga cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta
    20641 ctgcgggcat gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg
    20701 tgagcgttac tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc
    20761 gatcctcctc gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt
    20821 cgtcgaagtg ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg
    20881 aattaaagtt aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt
    20941 aatgaccgcg tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt
    21001 gctcgaggcg aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt
    21061 agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg gcagagtatg agtgatgata
    21121 cactgaccgc ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta
    21181 tctgagggcg ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc
    21241 gggtcgctat ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct
    21301 gtgcaaaaac gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct
    21361 ggatgcggcg atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct
    21421 ctattttgaa gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt
    21481 cgataaactg ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg
    21541 taccattcgt cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg
    21601 gacgctggcg acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa
    21661 aatgatgtat gccgatccgc aggcgctgca cgctctgctc gataaactgg cgaaaagcgt
    21721 cactttgtat ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac
    21781 ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa
    21841 aattgttgat ggtttagtgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac
    21901 caaaggcggc ggacagtggc tggaagccat ggcagaaacc ggttgcgatg cgctgggcct
    21961 cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca
    22021 gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc
    22081 gactatactt gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat
    22141 tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc
    22201 tgaacagtat caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat
    22261 cgaactggct tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat
    22321 cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct
    22381 gctgaaatat ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat
    22441 gccttacatt ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga
    22501 gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg
    22561 ccgtcttggc gtcgccagcc attttggctt attggtggat gtgccgacca ttggcgtggc
    22621 gaaaaaacgt ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc
    22681 gctgatggat aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc
    22741 gttgtttatc gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg
    22801 ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga
    22861 acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa
    22921 tttccgtatt tgagaactca acatgttaca aaacccaatt catctgcgtc tggagcgcct
    22981 ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta
    23041 cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga
    23101 tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga
    23161 gaaatttgaa gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc
    23221 aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct
    23281 cgaacacgcg gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac
    23341 tcaggctggt cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga
    23401 atgggacatt cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga
    23461 gctgataaaa ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat
    23521 ttttcagcaa taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt
    23581 ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt
    23641 gtatgcagga gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa
    23701 cacattgtaa ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc
    23761 agaactgtcc aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga
    23821 gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca
    23881 ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc
    23941 taacgtaccg gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt
    24001 ggcagtgaac agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc
    24061 attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc cacaactctt cacttcctcc
    24121 ctttaccgcc agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag
    24181 cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac
    24241 gcgagagtat cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa
    24301 accgccggtg aacattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg
    24361 tgaatccgat ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg
    24421 tgctgctcag atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca
    24481 ggggcgctgg catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc
    24541 tgatttagat tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga
    24601 tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc
    24661 tgatttctgt cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc
    24721 atatgaccgc caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata
    24781 cccgcttcag ccatcgcgat atcccgtttc acccgtaact catctaacga ctgacgcaaa
    24841 ttctccatct ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac
    24901 agggcattgt attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg
    24961 taaaagtcat tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca
    25021 ttttgctgcc acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt
    25081 gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca
    25141 gtggttgtct tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg
    25201 ggtaatctgg catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt
    25261 ttactcattg aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg
    25321 catgatttct gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac
    25381 gttctaaaga ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc
    25441 tggtagggtt gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca
    25501 gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg
    25561 tcggcatggg gatgcgcatg catcatgcgc agcagcaggc cttactggaa gaaatggccg
    25621 ggcagcctgg agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata
    25681 gcaactccgg catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc
    25741 cgggagatga agaagcgtgg cggcggatcg atagcgcaga cggcgagcct gttctggaaa
    25801 tttatcgcca gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc
    25861 agtatgccgc gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg
    25921 ccgaagatcg tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc
    25981 tggcaagcgt attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac
    26041 aagatgaaat gaagcgcaaa gagaagctag tggcgctggg gcatcttgcg gcaggcgttg
    26101 cccacgaaat ccgtaaccca ctttcctcga ttaaaggact ggcgaaatac tttgccgagc
    26161 gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc
    26221 gtttaaaccg cgtggtaagc gagctgctgg aactggttaa gccaacgcat ctggctttgc
    26281 aggcggtgga tctcaacacg ctgattaacc actcattaca gctggtaggc caggatgcaa
    26341 acagccggga gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg
    26401 atccggacag gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg
    26461 gtcagcatgg cgtgattagc gtgacggtca gcgaaagcgg cgcgggcgtg aaaatcagcg
    26521 ttaccgacag cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact
    26581 tcaccaccaa agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac
    26641 aacacggtgg tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct
    26701 ggcttccggt caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat
    26761 tctggtggtg gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg
    26821 gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggagcg
    26881 ggtttttgat cttgtgcttt gcgatgtgcg aatggcagag atggacggca ttgccacgct
    26941 aaaagagatc aaagcgttaa atccggcgat tccggtactg attatgaccg catactccag
    27001 cgtcgagacg gcggtagagg cactgaaaac cggggcgctg gattatctca tcaagccgct
    27061 ggatttcgac accctgcagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga
    27121 tgctgaaacg cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat
    27181 gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca
    27241 cggcgattcc ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg
    27301 tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc
    27361 tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg
    27421 ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat
    27481 gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa
    27541 ccagactatc tcggttgatg tccggctgat tgcggcgacc catcgcgatc ttgccgcaga
    27601 ggtgaatgcc gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga
    27661 agtaccatcg ctgcggcaac ggcgggagga tatccctctg ctggctagcc attttcttca
    27721 gcgttttgcc gagcgtaatc gcaaggcggt aaaaggcttt acgccacagg cgatggattt
    27781 gctgatccac tacgactggc cgggaaacat tcgtgaactg gaaaacgcgg tggaaaggtc
    27841 ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccag
    27901 tacgccgatc ccgctggggc aaagtcagga tattcagccg ctggtggaag tcgaaaaaga
    27961 agtgattctg gcggcactgg agaaaacggg cggcaacaaa accgaagccg cccgtcagtt
    28021 agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt tagttctgct cgcgttcgat
    28081 agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc cagtgaatat tggtcattaa
    28141 ggcataagcg cgtttctgcg cttctgccac ggtatgaccc agcgcggtga cgcacagtac
    28201 gcgcccgccg ctggtcacta cctgctcgtt atccgccagt ttcgtgcccg cgtggaacac
    28261 tttgccgcct gccacttctt ccagcggcag gccgtggatc acgtcaccgg tgcggtaatc
    28321 acccggatat ccacccgcag ccatcaccac gccgagagaa gcgcgttcat cccactctga
    28381 cgttttctcg tccagtttgc cttcacaggc cgccaggcag agctcaacca gatcggactt
    28441 catgcgcagc ataatcggct gggtttctgg atcgccaaag cggcagttaa attcgataac
    28501 cttcggattg ccctgtttgt cgatcatcag gcccgcgtag agaaaaccgg tgtaggtgtt
    28561 gccttccgcc gccatgcctt tcacggttgg ccagatgata cgttccatgg tgcgctgatg
    28621 aacttcatcg gttactaccg gcgcggggga gtaagcgccc atcccgcccg tgtttggtcc
    28681 ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg gccatcggca gcacatgctc
    28741 gccgtctacc atcacgataa agctcgcttc ttcgccatcg aggaactctt cgataacgat
    28801 gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc
    28861 ttcttccagc gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat
    28921 gacgattggc gcgcctttct cacgcagata cgccagcgca ggttctacct cggtgaagtt
    28981 ctggtattcc gccgtaggga tcttatggcg ggccaggaaa tctttggtaa acgcttttga
    29041 gccttccagt tgggccgcac ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa
    29101 ggtatcgacc acgcctttca ccagcggcgc ttccgggccg acgatggtca gatcaatctt
    29161 ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc acgccaatag caacgttttg
    29221 cagcgcgggt tccagtgcag tgcctgcatt acccggagca acaaaaacag tctcaaccag
    29281 cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa
    29341 tactttcatc tattgctcca ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca
    29401 ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg
    29461 attacgcagg tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac
    29521 gcgtcagaag ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg
    29581 gcgattttcg cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg
    29641 tttttggcat agacgatagc attggatttc acgaacttcg ccactttcca gcagaacagc
    29701 gcatcgcgca gttcctgttc ggtcggctga cgttgggtga cgacgcgcag ttcttccgca
    29761 ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca
    29821 agacccggaa cacgctcgcc ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg
    29881 gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa taatcacttc gacaaactga
    29941 cgagaaatga tggcctgtgc ggtttccgca tctagctcgc ggttaaaggc aatgatgccg
    30001 ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt aagcatcaag aatggaattg
    30061 ccgatagcta caccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac
    30121 tctttcacgc actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta
    30181 ccctgaacct gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag
    30241 gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg
    30301 ttcagcgtgc gtgggaagcg accagcggct tctttgcttt caccgtggta cgccggaacc
    30361 atgctgccga agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg
    30421 atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta
    30481 ataatggcgt cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttagcg
    30541 gcggagcgca ccatggtcgg gccgccgata tcgatgttct caaccgcatc ttccagcgag
    30601 caaccttcac gggccacggt ctgggcgaac ggatacaggt taacaaccac catatcgata
    30661 ggctggatct gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca
    30721 ccatgtactt tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg
    30781 taatcggaaa cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct
    30841 gtagacagca gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct
    30901 ttgtcagaaa cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc
    30961 ctggatttga ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc
    31021 ccctaacggg gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc
    31081 gcgaattttt ctctttcaat ggtgatcaca attttgacag tggttaccgt gggaaaaata
    31141 cagaaattac attgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt
    31201 tgctggggaa tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga
    31261 actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg
    31321 gttcggttga agagaaaaat cctgaaattc agggttgact ctgaaagagg aaagcgtaat
    31381 atacgccacc tcgcgacagt gcgctaaag
//
LOCUS       DAZFVO020000043        31219 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFVO020000043 DAZFVO020000000
VERSION     DAZFVO020000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 31219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.043"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            142..3858
                     /gene="yfaL"
                     /locus_tag="ACHKNB_RS09235"
                     /old_locus_tag="pgaptmp_001847"
     CDS             142..3858
                     /gene="yfaL"
                     /locus_tag="ACHKNB_RS09235"
                     /old_locus_tag="pgaptmp_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HGU4195376.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTLTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGNG
                     QTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVGGYSDNQ
                     GESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEQDDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDD
                     IQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(3986..4708)
                     /gene="ubiG"
                     /locus_tag="ACHKNB_RS09240"
                     /old_locus_tag="pgaptmp_001848"
     CDS             complement(3986..4708)
                     /gene="ubiG"
                     /locus_tag="ACHKNB_RS09240"
                     /old_locus_tag="pgaptmp_001848"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HGU4195377.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4855..7482
                     /gene="gyrA"
                     /locus_tag="ACHKNB_RS09245"
                     /old_locus_tag="pgaptmp_001849"
     CDS             4855..7482
                     /gene="gyrA"
                     /locus_tag="ACHKNB_RS09245"
                     /old_locus_tag="pgaptmp_001849"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HGU4195378.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7631..9318
                     /gene="yfaA"
                     /locus_tag="ACHKNB_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /pseudo
     CDS             7631..9318
                     /gene="yfaA"
                     /locus_tag="ACHKNB_RS09250"
                     /old_locus_tag="pgaptmp_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHKNB_RS09255"
                     /old_locus_tag="pgaptmp_001851"
     CDS             9315..9938
                     /gene="yfaT"
                     /locus_tag="ACHKNB_RS09255"
                     /old_locus_tag="pgaptmp_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HGU4195379.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9962..14476
                     /locus_tag="ACHKNB_RS09260"
                     /old_locus_tag="pgaptmp_001852"
     CDS             9962..14476
                     /locus_tag="ACHKNB_RS09260"
                     /old_locus_tag="pgaptmp_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HGU4195380.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLGDA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14477..15939
                     /locus_tag="ACHKNB_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /pseudo
     CDS             14477..15939
                     /locus_tag="ACHKNB_RS09265"
                     /old_locus_tag="pgaptmp_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
     gene            15944..16720
                     /gene="yfaP"
                     /locus_tag="ACHKNB_RS09270"
                     /old_locus_tag="pgaptmp_001854"
     CDS             15944..16720
                     /gene="yfaP"
                     /locus_tag="ACHKNB_RS09270"
                     /old_locus_tag="pgaptmp_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HGU4195381.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16794..17978)
                     /gene="atoB"
                     /locus_tag="ACHKNB_RS09275"
                     /old_locus_tag="pgaptmp_001855"
     CDS             complement(16794..17978)
                     /gene="atoB"
                     /locus_tag="ACHKNB_RS09275"
                     /old_locus_tag="pgaptmp_001855"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HGU4195382.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKIFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18009..19331)
                     /gene="atoE"
                     /locus_tag="ACHKNB_RS09280"
                     /old_locus_tag="pgaptmp_001856"
     CDS             complement(18009..19331)
                     /gene="atoE"
                     /locus_tag="ACHKNB_RS09280"
                     /old_locus_tag="pgaptmp_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HGU4195383.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19328..19978)
                     /gene="atoA"
                     /locus_tag="ACHKNB_RS09285"
                     /old_locus_tag="pgaptmp_001857"
     CDS             complement(19328..19978)
                     /gene="atoA"
                     /locus_tag="ACHKNB_RS09285"
                     /old_locus_tag="pgaptmp_001857"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HGU4195384.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(19978..20640)
                     /gene="atoD"
                     /locus_tag="ACHKNB_RS09290"
                     /old_locus_tag="pgaptmp_001858"
     CDS             complement(19978..20640)
                     /gene="atoD"
                     /locus_tag="ACHKNB_RS09290"
                     /old_locus_tag="pgaptmp_001858"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HGU4195385.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20836..22221)
                     /gene="atoC"
                     /locus_tag="ACHKNB_RS09295"
                     /old_locus_tag="pgaptmp_001859"
     CDS             complement(20836..22221)
                     /gene="atoC"
                     /locus_tag="ACHKNB_RS09295"
                     /old_locus_tag="pgaptmp_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HGU4195386.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22218..24044)
                     /gene="atoS"
                     /locus_tag="ACHKNB_RS09300"
                     /old_locus_tag="pgaptmp_001860"
     CDS             complement(22218..24044)
                     /gene="atoS"
                     /locus_tag="ACHKNB_RS09300"
                     /old_locus_tag="pgaptmp_001860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HGU4195387.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24211..27060
                     /gene="rcsC"
                     /locus_tag="ACHKNB_RS09305"
                     /old_locus_tag="pgaptmp_001861"
     CDS             24211..27060
                     /gene="rcsC"
                     /locus_tag="ACHKNB_RS09305"
                     /old_locus_tag="pgaptmp_001861"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HGU4195388.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27260..27910)
                     /gene="rcsB"
                     /locus_tag="ACHKNB_RS09310"
                     /old_locus_tag="pgaptmp_001862"
     CDS             complement(27260..27910)
                     /gene="rcsB"
                     /locus_tag="ACHKNB_RS09310"
                     /old_locus_tag="pgaptmp_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HGU4195389.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(27955..29244)
                     /locus_tag="ACHKNB_RS09315"
                     /old_locus_tag="pgaptmp_001863"
     CDS             complement(27955..29244)
                     /locus_tag="ACHKNB_RS09315"
                     /old_locus_tag="pgaptmp_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4195390.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITAN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(29278..29532)
                     /gene="xisR"
                     /locus_tag="ACHKNB_RS09320"
                     /old_locus_tag="pgaptmp_001864"
     CDS             complement(29278..29532)
                     /gene="xisR"
                     /locus_tag="ACHKNB_RS09320"
                     /old_locus_tag="pgaptmp_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4195391.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(29540..29686)
                     /locus_tag="ACHKNB_RS09325"
                     /old_locus_tag="pgaptmp_001865"
     CDS             complement(29540..29686)
                     /locus_tag="ACHKNB_RS09325"
                     /old_locus_tag="pgaptmp_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195392.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(29707..29949)
                     /locus_tag="ACHKNB_RS09330"
                     /old_locus_tag="pgaptmp_001866"
     CDS             complement(29707..29949)
                     /locus_tag="ACHKNB_RS09330"
                     /old_locus_tag="pgaptmp_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195393.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(29942..30844)
                     /locus_tag="ACHKNB_RS09335"
                     /old_locus_tag="pgaptmp_001867"
     CDS             complement(29942..30844)
                     /locus_tag="ACHKNB_RS09335"
                     /old_locus_tag="pgaptmp_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195394.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(30841..31065)
                     /locus_tag="ACHKNB_RS09340"
                     /old_locus_tag="pgaptmp_001868"
     CDS             complement(30841..31065)
                     /locus_tag="ACHKNB_RS09340"
                     /old_locus_tag="pgaptmp_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195395.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
BASE COUNT         7531 a         7676 c         8318 g         7694 t
ORIGIN      
        1 attatgacgc actgatacta ttcatcaaat aataacataa tagccattgc atcgggttgg
       61 acaatttaca aaagaaagat aacccatatt gtataatgcg tttaaatatg ccgtcttata
      121 taaaatgatg gttagatgaa tatgcggatt atctttctac gcaaggagta tttatctcta
      181 ctcccgtcaa tgattgcatc tcttttctct gctaacggtt tcgcggcggc cattgattta
      241 tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct ttcaggcatt
      301 acgcaggact ggagtgtcgc cgatgggcaa tggctggttt tttcggatat gaccaataac
      361 gccagcggag gagccgtatt tttgcaacaa ggagcggaat tttcactatt accagaaaat
      421 gaaactggaa tgactctgtt tgccaataac accgttacag gagaatataa taacggcggg
      481 gccatatttg ctaaagaaaa ctcaacgctg aatcttactg atgttatttt ttctggtaac
      541 gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaatgatac tggtgccgtc
      601 gatttacgtg tcactaacgc catgtttcgc aataatatcg ctaatgatgg caaaggtggc
      661 gcaatttata ccattaataa tgacgtctat ttaagtgatg ttatttttga taacaaccag
      721 gcatatacat taacaagtta cagtgatggc gatggcgggg caatcgatgt taccgataat
      781 aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc ctttacaaat
      841 aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc tccctatctt
      901 attgatattt ctgttgatga cagctacagc cagaacggcg gcgtgttagt ggatgagaac
      961 aacagcgcag caggctatgg agatggtcct tccactgcgg cgggtggctt tatgtatctc
     1021 ggcttaagtg aagttacctt tgatattgcc gacggaaaaa cgctggttat tggcaataca
     1081 gagaatgacg gagctgttga ctctattgct ggtaccgggt tgatcaccaa aacaggttcc
     1141 ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca gattgaaaac
     1201 ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac gcattgccag
     1261 gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta ccagaatcag
     1321 gcagagctaa acgttggctc cacccaacaa acctttgtgc actcattgac gggctttcag
     1381 aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg cagttttgct
     1441 ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta cgtgctgtcg
     1501 ggggcgcagt cgatggcgct aaccggcgat atagtcgttg atgatggtgc ggtgctttcg
     1561 ctggaaggcg acgcggcaga tctcgccgct ctccaggacg atccgcagtc gatcgtgtta
     1621 aacggcggtg tgctcgatct ctctgatttc tccacctggc agagcggcac atcatacaat
     1681 gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga tgtggtagat
     1741 cttgcaggtg gcgacaattt gcatatcggc ggcgacggga aagatggcgt ctacgtggtg
     1801 gtcgatgcga gcgacgggca ggtaagtctg gcaaacaata atagttattt gggcacaaca
     1861 caaatcgcct ccggtacgct gatggtgagc gacaactcgc agcttggaga tacccactat
     1921 aaccgccagg ttatctttac cgataagcaa caagaaagcg tgatggagat tactgccaat
     1981 gtcgatactc gctctacgac gactgagcat gggcgtgata ttgaaatgcg cgccgacggt
     2041 gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc tgacagcagc
     2101 gggcagcatc aggatgaggg tagcacattt actaaaacgg gggcgggtac gctggagctg
     2161 accgccagcg gtacaacgca gtcggcagta cgtgtcgaag aaggcaccct gaaaggcgat
     2221 gttgcggata tctttcctta tgcttcgtca ctgtgggtcg gtgatggggc gacgttcgtt
     2281 actggcgcgg atcaggatat tcagtctatc gatgctactt ccagcggcac tatcgatatc
     2341 agcgatggta cggttttgcg tctgaccggg caggatactt ccgtcgccct taatgcctca
     2401 ttgtttaacg gcgatgggac gctggtgaat gccaccgatg gcgtgacgtt gacaggtgag
     2461 cttaatacca accttgaaac tgacagcctg acctatcttt ccgacgtgac ggttaatggc
     2521 aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg cgatacgctg
     2581 acggtaaacg gtgattatac cggcggcggt acgctactgc tcgatagcga attaaacggc
     2641 gatgactcgg taagcgatca attggtgttg aacggtaata ctgctggcaa cacaactgtg
     2701 gtggttaact ccattacagg gattggtgag ccgacatcga caggcattaa agtggttgat
     2761 ttcgcagccg atcccacgca gtttcaaaac aatgcgaagt tcagtctggc aggcagcggc
     2821 tacgtcaata tgggagcata tgactacacg ctggtggaag ataacaacga ctggtatctg
     2881 cgatcgcaag aagtaacgcc accattgcca cctgatccag accctgaccc aacacccgat
     2941 ccaatccccg cttaccagcc ggtgctgaat gccaaagttg gcggttatct caataatctg
     3001 cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc atgcaggtgg caatggtcag
     3061 acgctgaatt tacgtgttat cggcggacgt tatcattaca cggcggcagg gcaactggct
     3121 cagcatgaag acacttctac ggtgcagctt agcggtgacc tgtttagcgg gcgctggggc
     3181 gatgatggcg agtggatgct tggggcggtt ggtggctaca gtgataatca gggcgagagc
     3241 cgctcgaata tgaccggaac tcgcgccgat aaccagaacc acggttatgc cgttgggctg
     3301 acctcaagct ggtatcagca cggtaatcag aagcaggggg cctggctgga tagctggctg
     3361 caatacgcgt ggtttaacaa tgatgtttcc gaacaagacg atggcacaga tcattaccac
     3421 tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt ggttaccggg gcgtggtgtg
     3481 gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc agcaggatga ttttaccgcc
     3541 gctaaccgtg cgcgcgtgtc acaatcgcag ggtgatgata ttcagatgcg gctgggttta
     3601 cacagcgaat ggcgtaccgc tgttcatgtc ataccaacat tagatctgaa ttattatcac
     3661 gatccccatt cgacggaaat tgaagaggat ggcagcacta tcagtgacga tgcggtgaag
     3721 caacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt gtccctgcgc
     3781 ggcagcgtgg cgtggcagaa agggagtgat gattttgccc agatggcagg gtttttgtcg
     3841 atgacggtga aatggtaatc actgtctagt cggcacggtt gccggatgcg gcgtaaacgc
     3901 cttatccggc ctacggggag catttgtagg cctgataaga cgcggcaagc gtcgcatcag
     3961 gcattggctc ggccaaacaa caacctcact tattctgcgt gtgcagcata tagttcacat
     4021 ccacgccggg gccgagttta aaagtattag tgagcgggtt gtaatgtagc ccggttatat
     4081 gtcgctcttt aaggcttgtc tgatctaccc agccaagcag ttctgccggt ttaataaact
     4141 tcttcacatc atgcgtgcct ttgggcacca tgcgcaaaat atattccgcg ccaaccaccg
     4201 ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa aaagacatcg ccgcctggtt
     4261 tcaccagttg cgcacaggct ctgaccactg actgcggatc ggggacgtgc tccagcatct
     4321 ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct tccacggttt
     4381 cctgcacata atccacctga atgccgcttt ccagcgcgtg cagttttgcc acctgcaatg
     4441 gctcaaagcc catatccaga ccggtcaccg tcgcgccttc gcgcgccata ctctcggcca
     4501 gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataaaccg ccagcacgct
     4561 cggcaatata gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac tcaccttcaa
     4621 gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg tctacgttat
     4681 ggttttccgg cgatttttcg gcattcattg gcacttctac tccgtaattg gcaagacaaa
     4741 cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa ctgcgcggct
     4801 gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg ttagatgagc
     4861 gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag ctcctatctg
     4921 gattatgcga tgtcggtcat tgttggccgt gcgctgccag atgtccgaga tggcctgaag
     4981 ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg gaacaaagcc
     5041 tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc ccatggtgac
     5101 tcggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg ttacatgctg
     5161 gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc aatgcgttat
     5221 acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga aaaagagacg
     5281 gtcgatttcg ttgataacta tgacggcacg gaaaaaattc ccgacgtcat gccaaccaaa
     5341 attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc aaccaacatc
     5401 ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga tgatgaagac
     5461 atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac ggcggcaatc
     5521 attaacggtc gtcgcggtat tgaagaagct taccgtaccg gtcgcggcaa ggtgtatatc
     5581 cgcgctcgcg cagaagtgga agttgacgcc aaaaccggtc gtgaaaccat tatcgtccac
     5641 gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga actggtaaaa
     5701 gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga cggtatgcgc
     5761 atcgtgattg aagtgaaacg cgatgcggtc ggtgaagttg tgctcaacaa cctctactcc
     5821 cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca tggtcagccg
     5881 aagatcatga acctgaaaga catcatcgcg gcgtttgttc gtcaccgccg tgaagtggtg
     5941 acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat ccttgaagca
     6001 ttagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca tgcgccgacg
     6061 cctgcagaag cgaaaactgc gctggttgct aatccgtggc agctgggcaa cgttgccgcg
     6121 atgctcgaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc agagttcggc
     6181 gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct ggatctgcgt
     6241 ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa agagctgctg
     6301 gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtttgat ggaagtgatc
     6361 cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac tgaaatcacc
     6421 gccaacagcg cagacatcaa cctggaagat ctgatcactc aggaagatgt ggtagtgacg
     6481 ctctctcacc agggctacgt taagtatcag ccgctttctg aatacgaagc gcagcgtcgt
     6541 ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga ccgactgctg
     6601 gtggcgaaca ctcacgacca tattctgtgc ttctccagcc gtggtcgcgt ctattcgatg
     6661 aaagtttatc agttgccgga agccactcgt ggcgcgcgcg gtcgtccgat cgtcaacctg
     6721 ctgccgctgg agcaggacga acgtatcact gcgatcctgc cagtgaccga gtttgaagaa
     6781 ggcgtgaaag tcttcatggc gaccgctaac ggtaccgtga agaaaactgt cctcaccgag
     6841 ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgacgg cgatgagctg
     6901 atcggcgttg acctgaccag cggcgaagac gaagtaatgc tgttctccgc tgaaggtaaa
     6961 gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac cggtgttcgc
     7021 ggtattcgct taggtgaagg cgataaagtc gtctctctga tcgtgcctcg tggcgatggc
     7081 gcaatcctca ccgcaacgca aaacggttac ggtaaacgta ccgcagtggc ggaataccca
     7141 accaagtcgc gtgcgacgaa aggggttatc tccattaagg ttactgaacg taatggttta
     7201 gtggttggcg cggtacaggt agatgactgc gaccagatca tgatgatcac cgatgccggt
     7261 acgctggtac gtactcgcgt ttcggaaatc agcatcgtag gccgtaacac ccagggcgtg
     7321 atcctcatcc gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt tgctgaaccg
     7381 gttgacgagg aagatctgga taccatcgat ggcagtgccg cggaagggga cgatgaaatc
     7441 gctccggaag tggacgttga cgacgagcca gaagaagaat aattttactt cttcatgcca
     7501 aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc tgggcttttg
     7561 tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc tatacacaag
     7621 gaatgtggca atgagtggtg aaaaaaaggc gaaaggctgg cggttctatg gtcttgtagg
     7681 ttttggcgca atagcactgc tttccgctgg cgtctgggcg ttgcaatatg ctggcagtgg
     7741 gccagaaaaa acgttgtcgc cgctggtggt gcacaacaat ctgcaaatcg atctcaatga
     7801 gccggacctc tttctcgaca gcgactctct gagccagctt cccaaagatc tcctcaccat
     7861 tccgtttctc cacgatgttc tgagcgaaga tttcgttttc tattatcaga atcatgccga
     7921 tcgtctgggc attgaaggca gcattcgtcg tattgtctat gaacacgatc tcacgctgaa
     7981 agataagctc tttcgtcact cttagatcag cccgcgcagg cagcgctgtg gcacgataaa
     8041 caaggccatc tttcacatta tatggtgctg atccagcgca gtggtttaag caaactgctg
     8101 gagccattgt tgtttgccgc taccagcgac agccagttaa gcaaaacgga aatcagtagc
     8161 atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg caataacgcc
     8221 ctgatgttcg cgacttatca ggacaagatg ctggtgtttt ccagcacgga tatgttgttt
     8281 aaagatgatc agcaggatac cgaagccacg gcgatcgcag gtgatttgtt gagcggcaaa
     8341 aaacgctggc aagccagctt cggtctggaa gagcgtactg ccggaaaaac accagtacgc
     8401 cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcgatt aatgccttct
     8461 tttgccggag tgcgtttcga aatgagcaac gacggctggc atagctttct ggcgttaaat
     8521 gatgaatccg cgagtgtgga tgccagtttc gattttacgc cggtctggaa cagtatgcct
     8581 gccggggcca gcttctgtgt ggcggtgccg tattcacacg gtattgccga agagatgctt
     8641 tcgcacatca gccaggaaaa cgacaagctg actggggcgt tagacggtgc cgcggggctg
     8701 tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt tgatggcact
     8761 gccgaacaag cgcaattgcc agggaaactg tttacgcaaa atattggtgc gcacgaaagc
     8821 aaagcgccag aaggtgtttt gccggtaagc cagactcagc agggcgaagg gcaaatctgg
     8881 cgtcgcgaag tgagttcccg atacggccag tatccgaaag cgcaggcggc gcaacccgat
     8941 caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt
     9001 ttctccctcg atgacacgct ggttaataac gcgctgcaaa cactgaataa aacccgcccg
     9061 gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa tccacaaggc
     9121 gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct cgaaccggtt
     9181 ttttataacg ccgcacaaac tttattaatg ccgaagctgg acgctttatc tcaacaaccg
     9241 cgttatgtca tgaagctggc ccagatggaa cccggtgccg cctggcagtg gttacccata
     9301 acctggcagc cgctatgagg cacgggctgc tggcgctgat ttgctggctg tgttgtgttg
     9361 ttgcccatag cgaaatgctg aatgtcgaac aatccgggct gtttcgcgcc tggtttgtac
     9421 gcattgcaca agaacagctc cgccaggggc caagtccacg ctggtatcag caggattgtg
     9481 cgggcctggt gcgatttgcg gcgaacgaga cgctgaaagt tcacgatagt aaatggctaa
     9541 aaagtaacgg tttctctagc cagtatttgc cgccagagat gacgctaaca cctggacagc
     9601 gtcaactggc gcaaaactgg aatcagggga acgggaaaac cggcccctac gtgaccgcga
     9661 ttaatttgat tcagtacaac agccagttta ttggccagga cataaaccag gcgctgcctg
     9721 gcgatatgat ttttttcgat cagggcgatg cccagcactt aatggtctgg atggggcgtt
     9781 acgtcatcta ccacaccgga agcgccacga aaactgacaa cggaatgcgc gcagtcagtc
     9841 tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
     9901 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
     9961 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgcgccgcc
    10021 cgccggggga acattctttt tgcttgctga cagcagtttt agcagcagtg aagaggcgaa
    10081 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
    10141 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
    10201 cctgcatcgc attgtggtgc aaccgcaata tctgggcgac gggctgaaca atacgctgac
    10261 ctggctgtgg gataactggt acggcaaatc tcgccgcgta atgcagcgta ctttctcttc
    10321 tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca atgccattat
    10381 taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat atcccttggt
    10441 ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc aaggggtaaa
    10501 actggaaggc gcatccagca atttcatctc gccgcagccg ggtaacattt atattcctct
    10561 cggccaacaa gagccgggac tgtacctcgt cgaggcgatg gttggtgggt atcgggcgac
    10621 gacggtggtg tttgtttccg ataccgtggc gcttagcaaa gtgtcaggca acgagcttct
    10681 ggtgtggacc gcgggtaaaa aacagggtga agcgaagccc ggctcagaga tcttgtggac
    10741 tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac agcggtacct tgcagttaca
    10801 acatatatcg ccagaacgct catacattct gggtaaggat gctgaaggcg gcgtttttgt
    10861 ctccgagaac ttcttttacg aaagtgaaat ctacaacacc cgcttgtata tctttaccga
    10921 tcgcccgcta tatcgcgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
    10981 cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg tgctggacgc
    11041 caacggcagt ctgttgcaaa ccgtcaatgt cacgctggat gcgcgcaatg gcgggcaggg
    11101 aagtttccgc ctgccagaaa atgccgttgc cggaggttat gagttacgtc ttgcttaccg
    11161 caatcaggtc tatagcagca gttttcgcgt ggcaaactac atcaagccac atttcgagat
    11221 tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
    11281 actgctctac ccggatggcg agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc
    11341 tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct
    11401 ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc
    11461 cgataaaccg agccgttatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgtgt
    11521 caccaccacc aaagagatcc tcattgaacg cggtctggcg cattactcat taagtactgc
    11581 cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc
    11641 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg
    11701 agagctaccg tcaggcggca aatcctttac cgtcaatttc gctaaacctg gcaactacaa
    11761 tctgacatta cgcgataaag acggcttaat tctcgctggg ttaagtcatg ccgtcagcgg
    11821 taagggcagc acggcgcata ctggtacggt agatatcgtg gcggataaaa cgctgtacca
    11881 gccaggcgaa actgcgaaga tgctgattac ctttccggag ccaattgatg aagcattatt
    11941 gacgctggaa cgcgatcgcg tggaacagca gtcgctgctt tcgcatccgg caaactggct
    12001 gacgttacaa cgtttaaacg atacccagta tgaagcccgg gttccagtga gcaattcctt
    12061 tgcgcctaac atcacttttt cggtgctgta tacccgtaat ggtcagtaca gttttcagaa
    12121 cgccgggatc aaagttgccg ttcctcagct tgatatccgg gtgaaaacgg acaaaaccca
    12181 ttaccagcct ggtgaactgg tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt
    12241 ttctgcgcag ctaacggtag gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat
    12301 cgcgccgaat atcggcaaat ttttctatcc gctggggcgt aacaatgtgc gtaccagctc
    12361 cagtctgtcg tttatcagct acgaccaggc gctctccagc gagccggttg cgcctggcgc
    12421 aactaaccgc agcgagcggc gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga
    12481 taccgcggca tggatgccgt cactcacaac cgataaacaa ggcaaagcgt atttcacgtt
    12541 cctgatgcct gattcgttaa cccgctggcg tatcaccgcg cgtgggatga acggcgacgg
    12601 gctggtcggg caggggcgtg cttatctgcg ttcggaaaaa aatctctaca tgaagtggag
    12661 tatgccaacg gtgtatcgcg tgggcgacaa accggcggca ggactgttta tcttcagtca
    12721 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggcgctgaga tgcgccagac
    12781 gctgacgctg cacaaagggg cgaattatat ttcgctggcg cagaacattc agcaatctgg
    12841 cttgttaagc gcagaactgc aacaaaatgg gcaagtgcag gacagcatta gcacaaaact
    12901 gtcttttgtg gataacagct ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg
    12961 cgagaacgcg ctgatgttgc ccgagcaggc tagcaatatc cggctacaaa gtagcgaaac
    13021 gccgcaggag attttccgca acaatcttga tgcgttagtc gatggaccat ggggtggcgt
    13081 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
    13141 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
    13201 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggtgacgc
    13261 cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
    13321 gcaacaaccg gaatactggc agcatatgct cgacagttat gccgagcagg cggataacat
    13381 gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa
    13441 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaag atgaagattt
    13501 ctctgaggaa gacatccgcg atatcaatga cagcctgatc ctcgatacac cggaatctcc
    13561 actgggagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa
    13621 gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagcaatca
    13681 gccgctggcg cacacggttg tgctgctcaa tagcggtggt gacgctaccc agacggccgc
    13741 tattttaagt ggtttgaccg ctgagcaatc cactattgag cgcgcgctgg ccatgaactg
    13801 gctggcgaaa tatatggcga caatgcctcc agttgttttg cctgcgcctg cgggcgcatg
    13861 ggctaaacat aagttaactg gagggggcga atactggcgt tgggttggtc agggtgtgcc
    13921 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
    13981 accggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
    14041 gcttatcccc ggtgaagaag agatgagctt tactctgcag ccggtgacca gcaatgagat
    14101 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
    14161 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
    14221 gggcatttca gtcaataaac ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc
    14281 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagaactga cgggaacggt
    14341 cactttccgc catttgctgc gcttctcgca aaaagggcaa ttcgttctgc ctcctgctcg
    14401 ttatgtgcgt tcctatgcac ctgcgcagca aagtgttgcg gcagggagtg aatggaccgg
    14461 gatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactct
    14521 gccaacgctt gcggaggaga cgccgttaca actggtgctg cgcggtgcgc agcacgatca
    14581 actgtatcag ctgtcgtcat caggcgtgac caaagtctca gcattacctg actcgctcac
    14641 cacgccgtta gggagtttgt ggaaactgta tgtttacgcc tggctggagg atacccatca
    14701 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
    14761 tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct attttgcgcc
    14821 gcagcggttg catattggcg cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc
    14881 tgcgtggtta gcgtctctgc ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct
    14941 gcttgattca ttagcgacgt tacccgcgca aaacaaagct caggaagtct tactggatgt
    15001 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
    15061 aacctggagc tgtttgcccg ctatccgctg aagaaaatca cagcggagaa gagtacgacg
    15121 gcagtaaaac ccggcgtact taatgggcgc tatcgtgtga cgtttaccaa tggtaaccac
    15181 attacgtttg tcagccacgg tgagacgacc ttactgagcg aaaagagcaa actgaaattg
    15241 cagtcgcatc tcgatcgtga agagtacgtt gccagagttc tggatcgcga ggcgaaaagt
    15301 acgccccctg aggcagcgaa agccatgacc gtggcgattc gcactttttt acagcaaaac
    15361 gccaatcggg aaggcgattg cctgaccatc cccgacagca gcgccacgca gcgtgtttct
    15421 gcttcgccag cgaccacggg ggcgcgaacg atgaccgcct ggacgcagga tctcatctat
    15481 gcaggcgatc cggttcatta tcatggcagt cgcgctaccg aagggacact ttcctggcgg
    15541 caagccacgg cccaggcggg gcagggggag cgttacgacc agatactcgc gtttgcttat
    15601 cccgacaaca gccttagccg ctggggagcg ccacgctcaa cctgccagtt attacccaaa
    15661 gcgaaagcct ggctggcgaa aaaaaagccg cagtggcggc gtatattaca agctgagacg
    15721 gggtacaacg aaccagacgt gtttgcggtt tgccatttag tctcaggttt cccctatacc
    15781 gatcggcagc agaaacggct gtttattcgt aacttcttta cgcttcagga tcggctggat
    15841 ttaacccatg agtatctgca cctggccttt gatggttatc ccaccgggct tgatgagaac
    15901 tatatcgaaa cgctgacccg acaattatta atggactaat gctatgcgaa agatttttct
    15961 tccgttgtta ctggtggcac tttcgcctgt cgttcacagt gaaggtgtgc aggaagtcga
    16021 gattgacgca ccgctctctg gctggcatcc ggtagagggc gaagatgcca gtttttcaca
    16081 atcgatcaat tacccggcgt cgtccgtcaa catggcagat gatcaaaata tctcagcgca
    16141 gatccgcggc aaaataaaga attatgctgc ggcgggtaaa gttcagcagg gccggctggt
    16201 ggtcaacggt gccagtatgc cgcagagaat tgaatccgat ggttcatttg cacgccctta
    16261 tattttcact gaaggcagca acagcgtgca ggtcatcagc ccggatgggc aaagccgaca
    16321 aaaaatgcag ttttactcaa cgccgggtac aggaacgatt cgtgcacgtt tacggctggt
    16381 tctctcgtgg gatacggaca ataccgatct cgatctccat gttgtcacgc ccgatggcga
    16441 acacgcctgg tacggtaaca cggtgctgaa aaacagtggt gcactggata tggatgtcac
    16501 gacggggtac ggacccgaga ttttcgccat gccagcgcca gttcacggac gctatcaggt
    16561 gtatatcaac tactatggcg gacgcagcga aacggaatta accaccgccc aactgacgct
    16621 gatcaccgat gaaggatcgg tcaatgagaa acaggaaaca tttattgtac cgatgcgtaa
    16681 tgctggcgag ctgacgctgg tgaaaagttt tgactggtga ttaatggggt taccttcgct
    16741 gtcgatgttc aagaaaacgc ccgataactt tcgctatcgg gcgtttttgt tcgttaattc
    16801 aaccgttcaa tcaccatcgc aattccctgg ccgccaccaa tgcacagtgt tgccagcccc
    16861 agcgttttat cgcgtgcctg catggcatgt aatagtgtga ccagaatacg agcaccactg
    16921 gcaccgatag gatgcccgag cgcgatggcc ccgccgttga cattcacttt ctcaggatca
    16981 aagcccaagg ttttcccaac ggcaaggaac tgtgcagcaa atgcttcatt agcttcgatg
    17041 agatcaatgt ctgccagttg cagccccgcc agttgtaacg ctttttgcgt ggcaggtact
    17101 ggccccatac ccatcaatgc gggaggcacg ccaccgctgg cataactttt aatgcgagcc
    17161 aggggggtaa ggcctgctgc cagcgccgca gattcttcca taatcaccag agcggcagca
    17221 ccgtcgttaa taccagacgc gttcccagcg gtgactgttc ctgctttatc gaaggccggg
    17281 cgcaatgcac ctaacgcttc agccgttgaa ttcgctttcg ggaattcgtc ttgactgaag
    17341 acgaagattt tcttccgagt gacaacattt accgggacga tttcggctgt aaaagcaccg
    17401 gactcaattg cggctgccgc tttacgctgt gaatgtagcg ccagttcatc ctgcatttca
    17461 cgggtaattc cgtactcttt agccacgttt tcggcggtaa tccccatatg ataaccatgg
    17521 gtggcgcaca tcaggccatc gcgcaggatt acgtcataaa cctgtccgtc tccaagacga
    17581 taaccagagc gtgcttttgc atcgagtaag tagggcgcta aactcatatt ttccataccc
    17641 cccgccacaa tgctctgcgc ctgacctgcc tgaattgcct gggcggcaag cgccacactt
    17701 ttaagacccg aaccacacac tttattgacc gtgaatccgc acaccgtttc tgccagcccg
    17761 ctttttaaca gtgcctgacg tgccggattt tgccccaacc cggcttgcaa cacgttaccc
    17821 ataatcactt catcaatgtg tagtgaatcg atttttgcac gttcaatggc ggctttaatt
    17881 actgtcgccc ccaggtcgat ggcgctggtg gaagcgagtg aaccgttaaa actaccgata
    17941 gcagtacgta ccgcactgac gatgacacaa tttttcattt tatattcctt ctgtctgtag
    18001 ggttgccgtc agaacagcgt taaaccaatg acgaaaatga cgccggagaa gagcagggca
    18061 gtgatgcaat agcccatgat gtcgcggaca ccgagtccgg cgattgccag tgctggcagc
    18121 gcccagaatg gttgtgccat gttcatccat tgctcgccgt aggcgatcgc cattaccgat
    18181 ttaccgagat cagcgcccag cgcctgggct gcgggtatca cgaaaggtcc ctgaataacc
    18241 cagtgaccgc cgccagacgg aacggcgaag ttaatcagtg cagaactaaa aaaggtcatt
    18301 accgggaagg tgtctttgtt cgcaacattg atgaagaatt cggtaatgag tccacccaga
    18361 ccagaatgct ccatcatcag ttggatccca gcgtagaagg ggaattgcac cagaataccg
    18421 gcagtactgc gtgctgccgc gctgatagca cgcatataag ccattggcgt tttatgtagc
    18481 aacagacccg caatcataaa catcaggttg acggtattga tggtgatgtt gaagccatgt
    18541 tcgctgaagt acatcgccag gtaagcgata ccgagtgcgc cgatgatcaa cgtcagaatg
    18601 cggctttctt ccagtcgctc ggatggtggg gcatctttcg gtagctgctt ttgaaaatca
    18661 gcctcttcca tgagtagttt cggatcgata ctcaccacgt cagacggttt tggcatcatc
    18721 atgcgggtga taaatggcat caccacaatc aacgccacag tgatgaaaat gttaaaacca
    18781 ctgaacagag tatcgcccac cgggatcagc ccggcgatat gctcaaccgg gttgcccggt
    18841 gttgcagcca acagaggcat tgagccagag aagccgccac cccaggtgag aaaaccaatg
    18901 taggcgcagg caatgagcaa cggataatca gaaccgggta ctcggcgggc gacttcacgg
    18961 gcaaacattg cgccgacaac caaaccaaat ccccagttga tgacacaagc gactgaaccg
    19021 aagaaagtga ccagcatgac gccctgtacg ggcgtctttg cggcggaggc ggcagtacgc
    19081 agcaaacttt tcaccggagc agagctggca agggcatgac cggtaacgat gataagcgcc
    19141 atctgcatac caaacgccag caagttccag aaaccgtcac cccacatttt caccatgctg
    19201 atcggcgttt gtggtgttaa ccaaagcgcg atcacgaatg ttagcaatgt cagcaacatg
    19261 gcaaagatca gtggatcggg aagccaccgg ctgacaaaac gcgtcataaa acgcgatatg
    19321 cgaccaatca taaatcaccc cgttgcgtat tcagatcggc ggcgacttca aaccgagctt
    19381 ctgttttggc acgcacggtg gctaaatcac acccgtcggc aatttcggtg agccacattt
    19441 tgccgtcaat aaaacgaaag acagccagtt cagtaaccag catatgcacc gcatgttgcg
    19501 cagtgagtgg catggtgcag cggcgcaaaa tttttgctga accatctttg gcgcaatgtt
    19561 ccatggcgat gatcactttg cgcgacccgg tcaccagatc catcgcgcca cccataccgg
    19621 gcaccatttt cccaggcact acccagttcg cgaggtttgc ttcttcgtct acttgcaaac
    19681 cgccgagcac gcaggcatca atatgaccgc cacggattag cgcaaatgac atggcgctat
    19741 caaacatggc tgcaccgggt aaaacaccgc acggttgccc gccagcgttc accagatctg
    19801 gatgcgctgt cgtgaccggg cctaaaccga ggaagccgtt ttccgattgc agagtgatat
    19861 gaataccctc cggtaaataa ttggcgacca ttgtgggtaa accgatccct aagttaacga
    19921 tgtcaccatc acgaagctct tgcgccacac ggcgcgcaat acgttgtttc gcatccatta
    19981 tttgctctcc tgtgaaacga tgatgtggtc gataacggca ccaggggtga caatatggtc
    20041 aggttgcagc tcgccggttt cgaccagttc atctggctct accagcgtga tatcagccgc
    20101 aagggctatc agggggttaa agttgcgggc gctaagttga taggtcaggt tgccaagtgt
    20161 gtcgcaacga tgagcgcgaa ttagcgccag gtcggcgcgc agtgggcgtt cgagcagcca
    20221 agttttaccg tcgagtgtca gtgtctgttt gccttcctct acgacggtgc cgacacccgt
    20281 tggggtgaga aaaccaccaa gtccagctcc accacagcga atttgctcga ttagcgtacc
    20341 ttgcggcacc agaacgacgt ccatctcacc agatatcatg cgccgacctg tttccgggtt
    20401 ggtgccgata tgtgaagcaa tcactttgcg gactcgacca ttgacgatga gcggaccgat
    20461 gccggtatca acaaacgcgg tatcattggc tatcaatgtc aggtcgcgaa caccagattc
    20521 cagtaatgct tcaaccaggc gggatggagt gccaatcccc ataaatccgc ccaccatgat
    20581 ggtcatgccg tcacgaaaga agccggtggc gtcttgtaat gtcatcaatt ttgttttcat
    20641 tttttatccc tcttgcatac accgttgtgg gtacagtcag gcaatagcaa ggggagtgcc
    20701 agaatgagat tattggattt agttatttaa ctaattgaat atgaagggat aaattggatg
    20761 gggatttata aggagaatga gcggctatgc agaaaattgc gcactgtgca aatttctgca
    20821 tagcaagttt tggtgttata catccgccgg atcgataccg tattcctgga gtttatacat
    20881 caatgcacgg cgactgatgc ccagcattaa agcagtgcgg gttcggtttc cttcttgttg
    20941 ttccagcact tccataatga tgcgtttttc gacgcgttta atttcctctt ttaaattacg
    21001 ctcaccgaca ggggctgttt ttacctcgcc agcattacag actggctgac gaatctgtgg
    21061 cggaagatcc tcagaaaaaa tgatcgggcc tgaattcatc acgacggcgc gttcaataac
    21121 gttggaaagc tctcgaatat ttcccggcca tgaccaggcg gtaagcagtg acattgccat
    21181 cggatcgatg tcgataatat cgcgctgatt ctcactactg aatttttgca aaaagtgatt
    21241 agctaacagg gaaatatctt cccggcgatc gcgcagaggc ggcagtatta aatgaataac
    21301 gttaaggcga taaaagagat cttcacggaa ggtaccttct tttaccattg cctgcaagtc
    21361 gcggttggtg gcagcaatga tgcggatatc aacttttatg gtctgatggc cgccaatccg
    21421 ttcaaattcc cgttcctgta gaatgcgtag taatttggct tgtagtacca gcggcatttc
    21481 gccaatttcg tcgaggagca gagtaccttc gttggctcgt tcaaataatc cctgacgcaa
    21541 ggtttgtgca ccagtaaatg cacctttttc atgaccaaac agttcacttt cgagcaacga
    21601 ttccggcagc gccgcgcagt tgactttaat gaacggcccc tttgcccgcc gcgaattgta
    21661 gtgaatcgct ctggcaatca actctttccc ggtgccgctt tcaccgctaa tcaagacgct
    21721 ggcctgagaa agggcaattt tggcggtgtc tttgcagatg tccatcatcg ccgggctgtt
    21781 ggtgagaatg tgcccccatt gccagctggt gctcagtgcc tggtgcagat gacggatctc
    21841 ttttttcatt gactggagtt gtaaagcgcg ctgaacgatt aaattcaact catcgagatc
    21901 aaacggttta ataacatagt cgaaggctcc gcagcgtagc gcttcgacgg cggtttccac
    21961 ttccgcatag gccgtcatca gaataacggg tgtccgggtc tcatggctgc gcatctcctt
    22021 tagtgccttg atgccgtcca tctctggcat gcggatatcc atcaacacca catcagggtg
    22081 aatatcggca aacaggtgta atgctgtgcg tccgttgttc gcacaatgtg tttcgaatcc
    22141 ttgtagtgca aaagcggtgc tcagcatacg gcgaacatta tcttcatcat ccacaataag
    22201 gatgcgatta atagcagtca tacagtctga tttccctgcg ggttgatcgg taaaataagc
    22261 gtgaaggttg cgccgtagcc cggcaaactg gcgacgcgaa tatcaccctg atgggcatta
    22321 atgatgcgtt gacttaacgc cagaccaagc ccggttcctg aggctttggt ggtgaaaaag
    22381 ggatcgaaga tctttttttg cagcgagaga tcaatgccac agccgttgtc ctctatcgaa
    22441 atggcctgtt gtgagtcgct gtattgccag gtttgaatgc gaattttccc tcgtgcgctg
    22501 atagcctgga cggcattgat caggatattc agtagtacct gtttgagcag ttcacgatcg
    22561 gcgttaatcg ggcttaattc attatccagt tcgcttatga agtcgacccg cgcttgtacg
    22621 ccggcggttt gtaccagtac cagagtttct tcaaccaatg cattgaggct gacttgttgc
    22681 cattgactgt ggcgtggacg tgaaaattcg agcaattgct gaataacttt gttaattgaa
    22741 tcgatttctt tgagtactac ggacagatat tcctgatgta ttgggtcact ggtttgttgg
    22801 cgcaagatct gtacataacc acgaatagcc gttaacggat tacgtacttc atgcgcgacg
    22861 ccagccatca gctcacccag tgtggcgagg cgttctgctt gcgccatgcg gcgctgggtt
    22921 tctttgcggg cagttaaatc agagaaaatc accaaagcac ctatcatttc accgtgcgtg
    22981 ttatgaatac gactggtagt gacactgagt tcaatggtgc ggtcacgacc tggaaaactg
    23041 atctccagcg ccacatgttc ggtgccatgt tccagcgtat ccagtactgg actgtagaac
    23101 tgagtattgt cgaacaacat ggagtaaggc tgccctacca gttcatggcg ttgatagcca
    23161 gtgataactt ctgctgctgg gttcatggtg gttacatcac cctggcggtc aatggcaatg
    23221 acgccatcgg cagcgttttc aataatcaga tcgttaagtg tccgcgtttc acgcagtgcc
    23281 tgggcgaggt tattaacact ctgactgatt tgccccattt caccgggcaa ttgtggtaat
    23341 cgagtgggaa tattttgtgc cagagtcgag aggccatcgg tgatgatatc aatattggcg
    23401 ctcaggcgac gggagaaaag gacaatcaat agcaggctta tcagcaaacc ggcggtgagc
    23461 acaatgataa tcctcacatc cattttccag gcctggcggc gaatatcttc ggttaattca
    23521 ttggcccaga tatagccgag gatttcacca ttacgctcaa tggggagcat tgaattcaaa
    23581 atatcgccgc gcacctgcct gcctgaataa accaaagggg tatttgtacg catgacttcg
    23641 cgaccagggt gatctgcggc aatggtaacg ccgacattat tctgatatag cgctgaaggc
    23701 gcgtaggtga ttatcgcatc cagcattttg ttgtaataac cagcaccgat gccagggaag
    23761 gcgtgagtga tattttcggt aatgggggca agttctgcat ttaatgcgcg gatacgctcc
    23821 tcacgtggta agtcgatgta gagatcatag cgatcgccta gtgcctgatt aagcaggttg
    23881 accacggcag aaagtttttt ctctttttca gataagactg ctgaacgtcc ttccgtttct
    23941 acgatataac caatagtaag cgttgggaca atgaccatca ggattgccat caggatcatt
    24001 tgattgcgta agcggcgtgg ataaatccac ttcatataat gcatgctgaa atccactagt
    24061 cttgtccggt atatgacgat tatcagaggt taaggtgatg atttctcggc ggtgtatcat
    24121 attccagaga agagagaaca ttgcggtaac acgcttttac cgctacctta accacactcc
    24181 atcggtcacc tgaggcggag cttcgcccct ttgaaatacc ttgcttcttt tcgtacaacc
    24241 ctgaaagcct cgcgctacat gttcagagca ttggcgttag tgctctggct gttgattgct
    24301 ttttcatccg ttttttacat cgttaatgcg ttacatcagc gagaatcgga aattcgtcag
    24361 gaatttaatc tgagttccga tcaggctcag cgctttattc aacgcacctc tgatgtgatg
    24421 aaagagctga agtacatcgc cgaaaatcgc ttatcggcag aaaacggtgt gctttccccg
    24481 cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac cgctgtttgc cgactccgat
    24541 tgttccgcaa tgagtaacac ctggcgaggt tctctggagt cattggcgtg gtttatgcgc
    24601 tactggcgcg ataatttttc tgcggcttac gatctcaacc gggtattttt aatcggcagc
    24661 gataacctct gcatggccaa tttcggtctg cgtgatatgc cagtggaacg cgataccgcg
    24721 ttgaaagctt tgcatgaacg catcaataaa tatcgaaatg caccacaaga tgatagcggc
    24781 agtaacctct actggatcag cgaaggtccg cgccctggcg tcgggtattt ttacgcgttg
    24841 acgccagttt atctggcgaa ccggttgcag gcgcttttgg gtgtcgagca gaccatccgg
    24901 atggagaact ttttcttacc gggtacgttg ccgatggggg ttaccattct tgatgaaaat
    24961 ggtcataccc tgatttcgct taccggacca gaaagtaaaa ttaagggcga tcctcgctgg
    25021 atgcaggaac gctcctggtt tggctatacg gaagggttcc gggagctggt gctgaagaaa
    25081 aatctgccac cctcatcgct aagcatcgtg tattcggtgc cggttgataa ggtgctggaa
    25141 cgcattcgca tgttgatcct taacgcaatt ttgctgaatg tgcttgccgg agctgcattg
    25201 tttactctcg cgcggatgta cgagcgacgt attttcattc cggcggaaag cgacgccctg
    25261 cgactggaag aacatgagca gttcaatcgc aagattgtcg cctccgcgcc agtgggtatc
    25321 tgcattttgc gtaccgctga tggcgtcaat attttaagta acgaactggc gcatacctat
    25381 ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc aaattatctg tgggcagcag
    25441 gtcaattttg ttgatgtcct gaccagcaac aataccaatc tgcaaatcag cttcgtccat
    25501 tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg tggatgtttc ttcgcgcgtg
    25561 aagatggaag agtcgttgca ggagatggca caagcagcgg aacaggcgag ccagtcaaaa
    25621 tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc cgctgtatgg cattatcggt
    25681 aacctggatc tgttgcaaac caaagagtta ccgaaaggcg tcgatcggct ggtgacggca
    25741 atgaacaact cttccagcct gttgttgaaa attatcagcg atattctcga tttctcgaag
    25801 attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt caccgcgtga agtgatgaac
    25861 cacatcaccg ccaactattt accgctggtg gtacgcaagc agttaggctt gtactgcttt
    25921 attgaaccgg atgtgccagt ggccttaaat ggcgacccga tgcgtttaca gcaggtcatc
    25981 tccaacctgt tgagtaacgc cataaaattc accgataccg gctgtatagt tttgcatgtc
    26041 cgcgctgatg gcgattatct ctctatccgt gttcgcgata ccggcgtggg gattccggcg
    26101 aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg gaacgggcgt acagcgaaac
    26161 ttccagggga ccggtctggg tctggcgatt tgtgaaaaac tgatcagcat gatggacggc
    26221 gatatctcgg tagattcaga accgggaatg ggcagccagt ttaccgtgcg tattccgttg
    26281 tacggcgctc agtacccgca gaaaaaaggc gtagaagggt tgagtggtaa acgctgctgg
    26341 ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa ccagtttgca gcgcagcggc
    26401 atcgtcgtta caacatacga agggcaggaa ccgactcccg aagatgtgtt aattactgac
    26461 gaggtagtga gtaaaaaatg gcagggtaga gcggtagtga ccttctgtcg tcgccatatt
    26521 ggtattccgc tggagaaagc gccaggggag tgggtacaca gtgtggctgc cccgcatgag
    26581 ctaccggcat tgttggcgcg tatttatttg atcgagatgg agagcgacga tcctgctaac
    26641 gctctgccgt cgacggacaa agcggtcagc gataatgacg atatgatgat tctggtcgtg
    26701 gatgatcatc cgattaaccg acgtctgctg gcagatcagt tgggatcgtt gggctatcaa
    26761 tgtaaaaccg cgaatgatgg tgtcgatgcg cttaatgtac ttagcaagaa tcatattgat
    26821 atcgtgctta gcgacgtcaa catgccaaat atggatggtt accgcttgac gcaacgcatt
    26881 cgtcagttgg gactgacgtt gccggtaatc ggagtaactg ctaatgcgtt ggctgaagag
    26941 aagcagcggt gtctggagtc cggtatggac agctgcctgt cgaagccggt aacgctggat
    27001 gtgataaaac agacgctgac ggtatatgcc gagagggtca ggaaatcgcg ggaatcgtag
    27061 gacggataag gtgtttatgc cgcatctggc attcagtgca aatgccagat gcgacgctga
    27121 cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat aatgggaatc gtaggccgga
    27181 taaggcgttt acgctgcatc cggcaatcgg tgcaaatgcc agataagaca ctaacgcgtc
    27241 ttatctggcc tacagatgat tagtctttat ctgccggact taaggtcaca gaagagagat
    27301 aattcagcag ggcgatatcg ttctcgacac ccagcttcat catcgcagat ttcttctggc
    27361 tactgatggt tttaatactg cggttcagct ttttagcgat ctcggtcacc aggaagcctt
    27421 ccgcaaacag gcgcagaact tcactctctt ttggcgagag acgcttgtca ccgtaaccac
    27481 cagcactgat tttttccaac aggcgagaaa cgctttccgg ggtaaatttc ttccctttct
    27541 gcagcgcagc gagagctttc ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt
    27601 cgatatccag atccaatacc gcactaagaa tcgccgggtt gttgttcatg gtcagaacaa
    27661 tgatcgacag gcttgggaaa tggcgcttga tgtacttgat taaggtaatg ccatcgccgt
    27721 acttatcgcc aggcatggag agatcggtaa tcaacacatg cgcatccagt ttcggcaggt
    27781 tgttgatcag tgctgtagag tcttcaaatt cgccgacaac attcacccac tcaatttgct
    27841 caagtgattt gcgaataccg aacaagacta tcggatggtc atcggcaata attacgttca
    27901 tattgttcat gtacttgtac ctatgtatta actatttgat tttaaaaata tattctatgc
    27961 tttgtttagt cgttggggca cctgtgggac ataagtacat agctttctgt ttagtacttc
    28021 tacctgggac tggttattgt ctttcatcca tgcaccgtaa acattgtaaa ccatctgggc
    28081 attcgcatgt cccatctggt tggctatgaa gtttggattt gctccggcag ttaaagccca
    28141 gcaagcataa gtatgccgag actgatacgc ttttctatgg cgaatttttg ctcgttttaa
    28201 tgcagattcc catgttctgt tgatggatgt tacggcataa tgaactcctg agttacctgt
    28261 gcgatctaat atctgaggat tgaaaacaaa gctgcatgag tgagtgcttg tccggccata
    28321 ctctcgcagc tttacttcta tctgatattg tttaccaagc ctggttaatg tcgcctgatt
    28381 ttttaatacg tcaattgctg gctgtatgag ttgtattacc ctgtttgttc ctgcatctgt
    28441 tttgggtagt gtaaattcct ttgtctgagt gtaattcctt ttgacggtca aggtacccgc
    28501 ttccagatcg atatcctccc atgccagccc acacagttcg ccatgccgca tcccggtata
    28561 tacagccagc gaccagaaat ttttagtttg ctggtggtgg cacgcatcaa tcagtctggc
    28621 aaactcatct tttgttagtg gatcgggagg tgttttcgct cttttcagtg atggaagctc
    28681 actgaaaggg tttttgccta tatacccatt gtttgccgca aaacggaaca tcccggagat
    28741 aattgtcata tagctattta cagtaggtac actacgacct ttaataggct tcctctgatt
    28801 ttttcctggc aactgatatc ccgttaataa ttctttacga aaaatgagaa catcttcctg
    28861 tgtaattgat gatgccagtt tgttccctcc aaggcagtca aggcaatttc ggatagctga
    28921 tttgtatctg accatggcat ttgctgttat ttccattttc ttaagctcaa gccatttttc
    28981 cgaaagtgcc ttaatggtta tctctttttt tcccagacca aaatgtttca ggttagggga
    29041 attagggaat tgcgcggcgt agtcgaagct ccccattctt attgcaaaac aaacggaagt
    29101 gcgaagttca cctgcgatct tccggttttt ggcggtgtca ggaacaccga ggttttctct
    29161 gacacgtttg ccgttatagt gaaaccatat gcggagtgat ccaccatggt tttcaacgcc
    29221 tgtcgggtat gatgcgttac tcattaaacc tcccagacgt ccaggagcat taacaggtta
    29281 accggaactt gcatttttgg cacctggttg tttctggttt tcgatccagc gcataatttc
    29341 ctcaatgttg tatacacatt cactgaaagg cccgggctct ccttctgctg cgtaatgacg
    29401 gtattctttc cccagtaacc aggatttttt tcttgctctg gcaatggttc ctgatttcag
    29461 tccagttgca gcaataaggg ttctttctgt gcaccatttg ctgggcgtta tctgatagat
    29521 gattgtttcc attcctgact cacactatct tcaggccacg gcagtggcac cacacgtcaa
    29581 acattcgctt tacaacttca cggcagtaga agccgtgagc atctctggtt aaatcgtatc
    29641 ggtacccgta tctgagtcgt atccatattt caaactcgcg attcacgact tattctcctg
    29701 ctgtctttat gccagttcat ccggttgcgc tggggcccag gccttccgtg gcctgtattt
    29761 gcggcgtttt ctggcttcaa tgcgtatctt tgtcaccctg actggctttg gttcgtgccg
    29821 gaacactttg tttctgatat tacgccgtgt attcatcatc cagataaaac gctgggtatg
    29881 gtcgcatccg tcatcaacgg taattactgt gcggaccagt tcatcgttaa tatcaaccat
    29941 ttcacccatt cactgagccc tcatacgcga atttcgtggc ttccatcagt tgcgtccata
    30001 cccattcaag aaattgtgcg ttttctcctg agcggtcata ttcatcgacg ctgcggggct
    30061 tgatggtcgc cagtagtggc acaatccggt acttcatctg ttcggttaac ttgtccggta
    30121 caagtcgcca gccgttatat ttcgatggaa catggtggta ttcagccagg aagcgtcgta
    30181 tatcgttttt catttcctcg catgagttct gatcatgaag tgcagcggaa agccagcgtc
    30241 cgatatattc agggattcgc acatcaagta ctttttctgc cgtcgtcagc attgcctgtc
    30301 ggcaggcgtt ccagccgttg acatagtgac gtgttgcatc ccatgaaacc tgcacatcag
    30361 ggtaagcatc ctgttctgtt ctcactggcg gtatctgagg aacagtgaat agtggactga
    30421 cgatatattt atccagagag cctccccgct cttctgcaag actttcccac gatgtcattg
    30481 tggggaccct gtaacctgct tttttgtgcg tatacatcca tgccactctg ccgttatcat
    30541 ctgcggcggc aatgcgctgc caggcaggat aaagcggggt gtatgtttct ctgtcattac
    30601 gggcgtcagg catggcatca atactgcaaa cttcccccgt aaggttattc agccaggcct
    30661 tcgggctaat ctctgacgtt acgatttcat ccagtttttt ctccagattt tctatgtgac
    30721 tgattagcgc aaggataact ggcggagtgg caagggagtg aaatgcttcc agatcacatc
    30781 ccttgtctcc atatacggta ttttgtgcgg cgtcacgcag tgctttcaga tcagcttctt
    30841 tcatcgttca gactcccttg ttgctgtttt gtaggcgcgc aggatgtcgc gtgtttttcc
    30901 tgataatgac gacttaacca ggaaaaagcc gctccggctt tctgtgatgt ccggcgtgca
    30961 cagaagtacc gcatcaacga cacgattatg ttttcgcaat gtcaggcggg aactggtgat
    31021 aatcagtttt gcctgttcgc cgtaatcggt gaatgaaata atcatctgat ggcgatctcc
    31081 gttatgaaag tgttcctgct ggttcaatgc tgataatttc tgctgcaata agcatacctg
    31141 ccagccatgc ttcgccgagc atgtcctcat ccgtacatgc aagatcaccg atctgtattg
    31201 tggccataat gttagtttc
//
LOCUS       DAZFVO020000044        30842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFVO020000044 DAZFVO020000000
VERSION     DAZFVO020000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.044"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            135..704
                     /gene="yadM"
                     /locus_tag="ACHKNB_RS09345"
                     /old_locus_tag="pgaptmp_001869"
     CDS             135..704
                     /gene="yadM"
                     /locus_tag="ACHKNB_RS09345"
                     /old_locus_tag="pgaptmp_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4195396.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            719..1321
                     /gene="yadL"
                     /locus_tag="ACHKNB_RS09350"
                     /old_locus_tag="pgaptmp_001870"
     CDS             719..1321
                     /gene="yadL"
                     /locus_tag="ACHKNB_RS09350"
                     /old_locus_tag="pgaptmp_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HGU4195397.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            1348..1944
                     /locus_tag="ACHKNB_RS09355"
                     /old_locus_tag="pgaptmp_001871"
     CDS             1348..1944
                     /locus_tag="ACHKNB_RS09355"
                     /old_locus_tag="pgaptmp_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4195398.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIVLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            1928..3271
                     /locus_tag="ACHKNB_RS09360"
                     /old_locus_tag="pgaptmp_001872"
     CDS             1928..3271
                     /locus_tag="ACHKNB_RS09360"
                     /old_locus_tag="pgaptmp_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HGU4195399.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            3384..4178
                     /gene="panB"
                     /locus_tag="ACHKNB_RS09365"
                     /old_locus_tag="pgaptmp_001873"
     CDS             3384..4178
                     /gene="panB"
                     /locus_tag="ACHKNB_RS09365"
                     /old_locus_tag="pgaptmp_001873"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HGU4195400.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            4190..5041
                     /gene="panC"
                     /locus_tag="ACHKNB_RS09370"
                     /old_locus_tag="pgaptmp_001874"
     CDS             4190..5041
                     /gene="panC"
                     /locus_tag="ACHKNB_RS09370"
                     /old_locus_tag="pgaptmp_001874"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HGU4195401.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(5115..6016)
                     /gene="rpnC"
                     /locus_tag="ACHKNB_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /pseudo
     CDS             complement(5115..6016)
                     /gene="rpnC"
                     /locus_tag="ACHKNB_RS09375"
                     /old_locus_tag="pgaptmp_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
     gene            6290..6670
                     /gene="panD"
                     /locus_tag="ACHKNB_RS09380"
                     /old_locus_tag="pgaptmp_001876"
     CDS             6290..6670
                     /gene="panD"
                     /locus_tag="ACHKNB_RS09380"
                     /old_locus_tag="pgaptmp_001876"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HGU4195402.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(6674..7903)
                     /gene="yadE"
                     /locus_tag="ACHKNB_RS09385"
                     /old_locus_tag="pgaptmp_001877"
     CDS             complement(6674..7903)
                     /gene="yadE"
                     /locus_tag="ACHKNB_RS09385"
                     /old_locus_tag="pgaptmp_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HGU4195403.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(7967..8407)
                     /gene="yadI"
                     /locus_tag="ACHKNB_RS09390"
                     /old_locus_tag="pgaptmp_001878"
     CDS             complement(7967..8407)
                     /gene="yadI"
                     /locus_tag="ACHKNB_RS09390"
                     /old_locus_tag="pgaptmp_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4195404.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(8512..9282)
                     /gene="yadH"
                     /locus_tag="ACHKNB_RS09395"
                     /old_locus_tag="pgaptmp_001879"
     CDS             complement(8512..9282)
                     /gene="yadH"
                     /locus_tag="ACHKNB_RS09395"
                     /old_locus_tag="pgaptmp_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4195405.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(9279..10205)
                     /gene="yadG"
                     /locus_tag="ACHKNB_RS09400"
                     /old_locus_tag="pgaptmp_001880"
     CDS             complement(9279..10205)
                     /gene="yadG"
                     /locus_tag="ACHKNB_RS09400"
                     /old_locus_tag="pgaptmp_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4195406.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            10314..10976
                     /gene="can"
                     /locus_tag="ACHKNB_RS09405"
                     /old_locus_tag="pgaptmp_001881"
     CDS             10314..10976
                     /gene="can"
                     /locus_tag="ACHKNB_RS09405"
                     /old_locus_tag="pgaptmp_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HGU4195407.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(11017..11553)
                     /gene="hpt"
                     /locus_tag="ACHKNB_RS09410"
                     /old_locus_tag="pgaptmp_001882"
     CDS             complement(11017..11553)
                     /gene="hpt"
                     /locus_tag="ACHKNB_RS09410"
                     /old_locus_tag="pgaptmp_001882"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGU4195408.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            11759..14149
                     /gene="gcd"
                     /locus_tag="ACHKNB_RS09415"
                     /old_locus_tag="pgaptmp_001883"
     CDS             11759..14149
                     /gene="gcd"
                     /locus_tag="ACHKNB_RS09415"
                     /old_locus_tag="pgaptmp_001883"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HGU4195409.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(14196..15746)
                     /gene="cueO"
                     /locus_tag="ACHKNB_RS09420"
                     /old_locus_tag="pgaptmp_001884"
     CDS             complement(14196..15746)
                     /gene="cueO"
                     /locus_tag="ACHKNB_RS09420"
                     /old_locus_tag="pgaptmp_001884"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HGU4195410.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            15912..16259
                     /gene="yacC"
                     /locus_tag="ACHKNB_RS09425"
                     /old_locus_tag="pgaptmp_001885"
     CDS             15912..16259
                     /gene="yacC"
                     /locus_tag="ACHKNB_RS09425"
                     /old_locus_tag="pgaptmp_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HGU4195411.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            16365..17231
                     /gene="speE"
                     /locus_tag="ACHKNB_RS09430"
                     /old_locus_tag="pgaptmp_001886"
     CDS             16365..17231
                     /gene="speE"
                     /locus_tag="ACHKNB_RS09430"
                     /old_locus_tag="pgaptmp_001886"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGU4195412.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            17247..18041
                     /gene="speD"
                     /locus_tag="ACHKNB_RS09435"
                     /old_locus_tag="pgaptmp_001887"
     CDS             17247..18041
                     /gene="speD"
                     /locus_tag="ACHKNB_RS09435"
                     /old_locus_tag="pgaptmp_001887"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HGU4195413.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(18079..18441)
                     /gene="yacL"
                     /locus_tag="ACHKNB_RS09440"
                     /old_locus_tag="pgaptmp_001888"
     CDS             complement(18079..18441)
                     /gene="yacL"
                     /locus_tag="ACHKNB_RS09440"
                     /old_locus_tag="pgaptmp_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HGU4195414.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(18617..21214)
                     /gene="acnB"
                     /locus_tag="ACHKNB_RS09445"
                     /old_locus_tag="pgaptmp_001889"
     CDS             complement(18617..21214)
                     /gene="acnB"
                     /locus_tag="ACHKNB_RS09445"
                     /old_locus_tag="pgaptmp_001889"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HGU4195415.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            21569..23326
                     /gene="yacH"
                     /locus_tag="ACHKNB_RS09450"
                     /old_locus_tag="pgaptmp_001890"
     CDS             21569..23326
                     /gene="yacH"
                     /locus_tag="ACHKNB_RS09450"
                     /old_locus_tag="pgaptmp_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HGU4195416.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(23397..24821)
                     /gene="lpdA"
                     /locus_tag="ACHKNB_RS09455"
                     /old_locus_tag="pgaptmp_001891"
     CDS             complement(23397..24821)
                     /gene="lpdA"
                     /locus_tag="ACHKNB_RS09455"
                     /old_locus_tag="pgaptmp_001891"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGU4195417.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(25146..27038)
                     /gene="aceF"
                     /locus_tag="ACHKNB_RS09460"
                     /old_locus_tag="pgaptmp_001892"
     CDS             complement(25146..27038)
                     /gene="aceF"
                     /locus_tag="ACHKNB_RS09460"
                     /old_locus_tag="pgaptmp_001892"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HGU4195418.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(27053..29716)
                     /gene="aceE"
                     /locus_tag="ACHKNB_RS09465"
                     /old_locus_tag="pgaptmp_001893"
     CDS             complement(27053..29716)
                     /gene="aceE"
                     /locus_tag="ACHKNB_RS09465"
                     /old_locus_tag="pgaptmp_001893"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HGU4195419.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(29877..30641)
                     /gene="pdhR"
                     /locus_tag="ACHKNB_RS09470"
                     /old_locus_tag="pgaptmp_001894"
     CDS             complement(29877..30641)
                     /gene="pdhR"
                     /locus_tag="ACHKNB_RS09470"
                     /old_locus_tag="pgaptmp_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HGU4195420.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         7610 a         8202 c         7504 g         7526 t
ORIGIN      
        1 taaatggctc gaagaaagta aacccgtaag ttgtcttgcg cattatcaac aaagttcaga
       61 agcagaaaaa atagcgcaat ctattattct gaatggaatt cggtgtcaga ttcagtaact
      121 acaagggacg tcaaatgata aaaataacgc cacataaaat agcgatactg atgggaatat
      181 tattatcacc atcagtattt gcaacggata ttaatgtaga gtttacagcc actgtcaaag
      241 cgacaacctg taacatcaca cttactggta ataacgtcac gaatgatggc aataataact
      301 acacattgag aatccctaag atgggtctgg ataagatcgc gaataaaacg acagaatctc
      361 aggctgattt taaactggtt gctagtgggt gcagcagtgg catcagttgg attgatacca
      421 ctctgaccgg aaatgcatca tcaagctcac ctaagcttat tataccgcag tctggtgatt
      481 catcttcgac gacaagtaat atcggtatgg gtttcaaaaa acggactact gatgatgcca
      541 ctttccttaa acctaacagt gcggaaaaga tacgctggag cacagacgag atgcagcccg
      601 ataagggtct tgaaatgacc gttgcgctac gtgaaacaga tgcagggcaa ggcgtaccgg
      661 ggaattttcg tgcactggca acgtttaatt tcatctatca ataagaagga taattaagat
      721 gacatttaaa aatttacgtt atggattatc cagcagtgtc gttttggctg cctcattgtt
      781 cagcgtaccc tcttatgcgg caacagacag cattgggctg accgtgatta ctaccgtaga
      841 aataggtact tgtactgcta cattagtaaa tgactctgat caggacattt ctgttgttga
      901 ctttggcgat gtatatattt ctgaaatcaa tgccaagacc aaagttaaaa cattcaaact
      961 caaattcaaa gactgtgcgg gtatccccaa taaaaaagca caaataaaat taaccaagcg
     1021 agccacatgc gaaggtactg ctaatgacgg tgcggggttc gcaaatggat ccacagccgc
     1081 agataaagca agtgccgtcg ccgttgaagt ctggagcacc gaaactccgg caacagggag
     1141 cgcaacacaa tttagttgtg tgacaccagc atctcaagag gtcactatct ccaatgcagc
     1201 caacgcggtt gtttattatc cgatgagtgc tcgactggtc gtcgaaaaaa ataaaaccgt
     1261 aagcaatgtc actgcgggta aattttctgc acctgccaca tttacagtaa cctataacta
     1321 acagactgac agatccagga actatatatg catcccactc aacgtaagct gatgaagaga
     1381 ataattctgt ttctgtcact acagtttccc attacctatc cagccatcgc tgggcaggat
     1441 attgaccttg ttgccaatgt aaaaaacagc acttgcaaaa gcggaatcag taaccagggt
     1501 aatattgttc ttggcgtcgt tggggtggga tatttttcag ataatgttac tcctgaaagc
     1561 tatcaaccag gtggaaaaga gttcactgtc acagtatcag actgtgcatt acagggaact
     1621 ggcgatgtgc ttaatcagtt acatattgat tttagagccc ttagcggtgt catggctgca
     1681 ggttcaaggc aaatatttgc caatgaagtc gcaaccgggg ctaaaaatgt cggtgtcgtt
     1741 ctcttttcca ttcaggaccc gaccaatata ttcaatgtta ttagttcagc aggaaattct
     1801 cgttccgtgt atccagtaat gacttctgcc ctgcataatt cttcatggaa attttatgcc
     1861 agaatgcaaa aaatcgatcc cgcgctggat gtcatatctg gccaggtaat gagtcatata
     1921 ttagttgatg tgtactacga ataatagatc aaaacaaata actgcgactg ttacagcagt
     1981 agatggtgga gtgataatga agaaattctt cagacatttt ctttttttaa ttctgtgctt
     2041 gagttgttat accgcgagtg ctggcactga tgataacgta ggatatattg ttgggaatag
     2101 ttacggcgtt ggtccgtcag atcaaaaatg gagagaaacg gggccgaatg gcgatgctac
     2161 tgtcatattc agatatgcaa caagtacaaa taatttagtt ttctacaaac cgacgcagct
     2221 tggacctaca ggtgtaaaat tacagtggag tcagttagat accgcttctg gtggtggttt
     2281 tctttattgc aacagaagtg atagcacaag tggtagcgca atgcgtattg aaaatgcaat
     2341 ggttgactca ggtaaaatgt atggctccca taaattattt aatacatcag ttcctggttt
     2401 gtattacaca ttattaattt caaacatgtg gtcagcttac ggtaccgtaa ctaacgttag
     2461 ttcacctggg atatacattg gtgactctgc agaacaatat ttttcgtggt ataatccaag
     2521 cgaagacgtg ttatattgga gttgcaataa tgcgaatagc acccgtaaat actgggctgt
     2581 aggtggtatt tatcagaccc ttacaattga attctataca gatacaaact ttgatccaac
     2641 agtaactcaa cagatcaaat tatccagctc atcaaattat ctgtattcat ttaaagccta
     2701 tggcgcaggc caaggtataa atgagcatag ttattttatc aaaatcgatt tcgatctgct
     2761 caatgtcaag ttgactaatc ccacttgttt taccgctatg cttagtggaa cttctgtgac
     2821 tggttctacg gtaaaaatgg gtgaatatag tgcagaacaa atcagaaacg gtgccacacc
     2881 ggttcctttt gatatttcac ttcaaaactg cgttcgtgtg actaatattg agacaaaatt
     2941 agtttcaaca aaggttggta ctgaaaacgg gcaactcctt ggtaatactc tcacaggtaa
     3001 tgacgcagca aaaggagtcg gtgtactcat agaaggttta gcaactagta aaaatcctct
     3061 aatgacattg aaacctaatg attcaaattc tgtttataaa gattacgacc cgagaggcaa
     3121 agacgataca acaggaggag tttacccgga tcaagataca ggtataacat accctctcca
     3181 tttccaggcc acgctacaac aggatggaac tataccaata gaagctggtg agtttaaagc
     3241 caccagtact ttccaggtaa cctaccctta ataagtccac cgcaccgcca tcatctggcg
     3301 gtgcggtaat tgataaatca ttgcccccac caatgtgact gataaaatgc caggcaacac
     3361 gacatcattt atcaggacac gttatgaaac cgaccaccat tgcttcactt cagaaatgta
     3421 agcaggataa aaagcgtttc gcgaccatca ccgcttacga ctacagcttc gcgaaactat
     3481 ttgctgatga aggacttaac gtcatgctgg taggcgattc gctgggcatg acggttcagg
     3541 gccacgactc cacccttccc gttaccgtcg aggatatcgc ctatcatacc gccgccgtac
     3601 gtcgcggcgc gccaaactgc ctgctgttgg ctgacctgcc gtttatggcg tatgccacgc
     3661 cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc cggtgccaat atggtcaaaa
     3721 ttgagggcgg tgagtggctg gtcgaaaccg taaaaatgct gaccgaacgt gccgttcctg
     3781 tatgtggtca cttaggttta accccacagt cagtgaatat tttcggtggc tacaaagttc
     3841 aggggcgcgg cgatgaagcg ggcgatcaac tgctcagcga tgcattagcc ttagaagccg
     3901 ctggggcaca gctgctggtg ctggaatgcg tgccggttga actggcaaaa cgtattaccg
     3961 aagcactggc gatcccggtt attggcattg gcgcaggcaa cgtcactgac gggcagatcc
     4021 tcgtgatgca cgacgccttc ggcattaccg gcggtcacat tcctaaattc gctaaaaatt
     4081 tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca gtatatggct gaagtggagt
     4141 ccggcgttta tccgggcgaa gaacacagtt tccattaagg agtcacgttg tgttaattat
     4201 cgaaaccctg ccgctgctgc gtcagcaaat tcgccgcctg cgtatggaag gcaagcgcgt
     4261 ggcgctggtg cctactatgg gtaatctgca cgatggccat atgaagctgg tcgacgaagc
     4321 caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt aacccgatgc agttcgaccg
     4381 cccggaagat ctggcacgtt atccacggac cttgcaggag gactgcgaga agctaaacaa
     4441 acgtaaagtg gatttagttt tcgccccttc ggtaaaagag atctacccga acggtactga
     4501 aacccatact tacgttgacg ttcccgggct ttcgaccatg ctggaaggcg cgagccgtcc
     4561 ggggcatttt cgcggcgttt caactatcgt cagcaagctg tttaacctgg tccagccgga
     4621 catcgcctgc ttcggtgaaa aggactttca gcaactggcg ctgatccgca aaatggttgc
     4681 cgatatgggc ttcgatattg agattgtcgg tgtgccaatt atgcgcgcca aagatggcct
     4741 tgcgctcagt tcccgtaatg gttatctgac ggcggaacaa cgcaaaattg cgcccggtct
     4801 gtacaaagtt ttaagttcaa ttgccgacaa attgcaggct ggggaacggg atctcgatga
     4861 aattattacc attgcggggc aagaactgaa tgaaaaaggc ttccgcgccg atgatattca
     4921 gattcgcgat gccgacacat tgctggaagt ttctgaaacc agcaaacggg cagtaattct
     4981 ggtagccgcc tggcttggcg atgctcgcct gatcgacaac aaaatggtcg agctggcgta
     5041 atacttaact ggcgctacgg ctgatggcgc cagttattaa tttaccccac gtcatctgtc
     5101 ataacacagg taacttaaat aaggttaatt accttatcaa tctcagcaag aggtaaattt
     5161 gccatctctg caacgtcttc ccgagacatt cctttactta gaagacgcag ggcgaattct
     5221 tgcctttcct cctgtcttcc ctgctgaatc cccttctcaa tccctttctc ttcaaaccac
     5281 tgcgccagcg tcatcataga ctcccctccc gtttccctgt ctctcagcac accataaaac
     5341 aaatccgctt gttcagtatg accgcgttgc agcatatagt tttgcatggc aactaactga
     5401 cttccgctag tgtacccttc gtcaatcagc gtgaccagtt gctccagcaa taacattaag
     5461 tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg caatccgccg atgttgcatg
     5521 atttcgtcat ccggtgtgat ggtgatatcc accagcggga aaggactgtt atagacgcgt
     5581 cgtgccagct ccggcgagta aaatatatca aaccagcaca ttgagagcgg ataaggtgtg
     5641 gcctcgccct gataaaacag tatcggcacc accagcggca gcttatcgtg aaccgcctcc
     5701 agatgccggt gcatggcagc aatagaataa cgcatcatgc gaaaggccat tttcttatcc
     5761 ggcttgcttt ggtgttcaat cacaacatgc agataaccgg gattgccctg catttgcacg
     5821 gaatagagca cgtccgtgct gtgtcctttc aggctctctt caatgaaact ccccgactct
     5881 aaatgaagcg tgttgaggtc acaaagttct cgtaattcca ctggcaaatg tatctccaga
     5941 aagtcgcgag ccgtctccgc atgcattaaa aattgttaaa taccgcgtca tgcggtgtgg
     6001 tactcggtgc atccatcttc cctgtcttcc gtgttgtctt gcgtgaaggc agtatccagt
     6061 caaggcgcgc cgtcgtcagc cgcctcttta gtgtttaccg agcagcgttc agagaaattt
     6121 caattcgtta caggcgatac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa
     6181 tgccatcaga tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca
     6241 aagcaggcac cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac
     6301 gatgctgcag ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg
     6361 ttcttgcgcc attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat
     6421 cgatatctgg aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg
     6481 cggttcgaga attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat
     6541 tgtcatcatc gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa
     6601 tgtcgcctat tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca
     6661 ggttgcttga ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg
     6721 aatccgttct taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt
     6781 tgcctttcat ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt
     6841 tgtcattaaa tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca
     6901 gagcgcggcg tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata
     6961 tgggtcggcg atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa
     7021 catcgcgaat ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact
     7081 tctgcgggtg acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt
     7141 gtttcaatac aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca
     7201 ccaccgctcg cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc
     7261 tcagtgtcgc gtatcccctg tcgcgcagcc aggccatctg gttattgaaa gcgcgtaccg
     7321 aggtggtcgt cgaagtatgg cgaaaacggg tgttttcttc atcgcgcaga atatggtgat
     7381 aggttagcac cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggcgagtc
     7441 gatcgccaat acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa
     7501 tcgggtagcg caaattgtcc gccagtaccc caaatggcgc actgcccgca ctcggcgcgt
     7561 tatagaccgg cgtatctttc caggtaatta agttctgatt actcagcggc ttgttgaggt
     7621 cacccaaacc gtcttcaact ttttgtcgcc cctgaactgg ctcgagatgc cctttatcga
     7681 taaacccttt accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg
     7741 caatgatttg cccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc
     7801 agatcgcagc attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg
     7861 cggtaaacag catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg
     7921 tgaaacaacg cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca
     7981 aattatgttt gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct
     8041 ccggcccacc cagttcgaca atacgctcgc gaaactctga actagtcatg gtttcacggc
     8101 aagccatcat ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt
     8161 gtttgtggct taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga
     8221 agatgacacc ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca
     8281 tattagagct taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt
     8341 ttttctccag cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac
     8401 ctaacatcgc cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg
     8461 tgatagccgt caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg
     8521 caaaccacgt ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac
     8581 caccagtacg ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg
     8641 gaaaccactg atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg
     8701 caacaaagtc agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt
     8761 tggcaccagg ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag
     8821 ggagaacaac accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg
     8881 aaacggcaca aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc
     8941 cacgccaccg ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag
     9001 ctcttcaata ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc
     9061 attggtgatc accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc
     9121 atgcatatcg ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat
     9181 ggtgatgact ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt
     9241 cgcccagatg ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt
     9301 tcattaacca gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat
     9361 acctgaatgc cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc
     9421 acttcaactt ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt
     9481 aacgggcttt tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc
     9541 gccttcatcg aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac
     9601 agcatttctg cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt
     9661 aaatccttca aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt
     9721 tcgtcgagaa tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc
     9781 ttcatcccgc cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt
     9841 ttaagatact tttcgctgcg gatgtacgct tctttgcgct ccacgccata gtaccctgcc
     9901 tgattcacca caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc
     9961 agtcccaact gacgtttagc gttcacgaca tccttctcga gatcgtaacc aaatacgctg
    10021 acccgcccgg aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg
    10081 gccccgttcg gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctatccca
    10141 cgaagcgcct gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg
    10201 gtcataaatt tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg
    10261 agttgcccta tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag
    10321 acatagatac actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc
    10381 ccgggttttt tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt
    10441 ccgacagtcg cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc
    10501 accgtaatgt tgctaacctg gtcattcaca ctgacctgaa ctgcctttcc gtggttcagt
    10561 atgcagtgga tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg
    10621 gcgtacaagc cgcagttgaa aacccggaac tggggcttat caacaactgg ctgctgcata
    10681 tccgcgatat ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc
    10741 tggatacctt gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca
    10801 ttatgcaatc agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca
    10861 ttcacgacgg cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc
    10921 aacgttaccg tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat
    10981 gccatgccgg atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt
    11041 gccgatatac ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa
    11101 ctcatccggg atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg
    11161 tttatccagc agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg
    11221 cactttcgac agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc
    11281 acggatatct tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct
    11341 accgtagctg gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag
    11401 gtccgccata aacataaatg agccacgcag cagaccaacc agcaccatat cgctgccgct
    11461 gtctttgtaa cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc
    11521 ttcggggatc attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa
    11581 atcacttcgc acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag
    11641 taaaactatt cattcgcgga atctgttaat aaagctcgaa ttgtgatgac gatcacacat
    11701 gttaaaacct ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat
    11761 ggcaattaac aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc
    11821 gctttgcggg ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg
    11881 gtactaccct atcgctggtc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa
    11941 acgtgccgcg ctttggctgt acgcagccct gctgctcggc accatgattt ggggcgtctg
    12001 ggaagttggt ttcgacttct gggcgctgac tccgcgcagc gacattctgg tcttcttcgg
    12061 catctggctg atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt
    12121 tgccgcactg gtggtggcac tgctgattag cggtggtatc ctgacctggg ccggatttaa
    12181 cgatccgcag gaaatcaacg gcaccttaag cgccaatgcc acacctgcgg aagctatctc
    12241 acccgttgcc gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc
    12301 acctctgaaa caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg
    12361 tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat
    12421 taaagtgggc gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc
    12481 cgccagcggc aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca
    12541 gcacgtaacc tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga
    12601 agtgatggcg gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc
    12661 gattaacgct gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct
    12721 gcaaagcaat atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat
    12781 caccgataaa accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac
    12841 gtctggcgtg atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc
    12901 cggcgcgaaa gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc
    12961 aaactcctgg gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg
    13021 cgtgaccacg ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag
    13081 ctcgattctg gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca
    13141 ccacgacctg tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa
    13201 tggtcagaaa gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga
    13261 tcgtcgcaat ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc
    13321 gaaaggcgat tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa
    13381 agatttgagc ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt
    13441 gatgttccac cagttgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct
    13501 ggtcttcccg ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg
    13561 tgaagtggca attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg
    13621 tccgggtaac ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat
    13681 tcagccacag tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg
    13741 tctgccgtgt aaacagccag catggggtta tatctcggcg ctggacctga aaaccaatga
    13801 agtggtgtgg aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgcctgt
    13861 accggtgccg ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa
    13921 cgtgctgttt atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg
    13981 tgaaaaactg tggcagggtc gtttaccagc gggtggtcag gctacgccaa tgacctatga
    14041 agtgaatggt aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa
    14101 gatgggcgac tatattgtgg cttatgcgct gccggatgat gtgaagtaac cataatgccc
    14161 ggagaaatcc gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc
    14221 cccgtatctt catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg
    14281 tcgtgattaa acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc
    14341 cagcccgcgc gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgggtg
    14401 ccgtgaatat ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg
    14461 tactgccctt tcgccgccgc aaacatcggc ttattcatat caaacgcctg accattgatt
    14521 ttattggcat ggtggaaatc gaacttcccg ccgtggttca tatgattcat attaccgtgc
    14581 cccatatggc ccatcatctg gctgtgatcc attccagcca tcgcctgatc gccatatttc
    14641 tccattagca tctgcatacc catcatatcg agcatcgggt ccatagaaag ttgcagcttg
    14701 cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtctggcaaa
    14761 gcaccggagg cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac
    14821 ggcgcaatcg ccatccccat ctggctgacc ggtagcgtca ccaggtcaaa gggtttgttg
    14881 tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc
    14941 ttcaccggtt caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg
    15001 ctggtggcga aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa
    15061 ccacgcgggg cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac
    15121 cagcccacgg cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat
    15181 tttttatcct gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat
    15241 ttcaggatct cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc
    15301 ccggtcttgc catgttgatg tggatggaac cagcaagtag cggcaggttg atcaacgttc
    15361 aacgtcaccg agcgcttgcc acctggcaga ataattccct gcgggccgcc gtcgacttca
    15421 cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt ccgtcagttg gttatagata
    15481 tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta
    15541 tagccccagg tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac
    15601 tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc
    15661 gccgcaaaga ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa
    15721 tattttaaga aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa
    15781 atcaaacgtt gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa
    15841 tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc
    15901 tggtagaagc aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgttt
    15961 gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag
    16021 tttttgtctt tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc
    16081 gcgcactggt ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg
    16141 acatgaaagc cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg
    16201 ctaaaaagtg caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat
    16261 ccctgatacc tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg
    16321 ttgcgccctt tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac
    16381 agtggcatga aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt
    16441 atcatgaaaa gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg
    16501 taatggcgct ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga
    16561 tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg
    16621 gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga
    16681 tggtggaaat cgatgcgggt gtcgtgtcgt tctgccgtca gtatctgccc aaccataacg
    16741 ccggtagcta cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta
    16801 atcaaaccag ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg
    16861 gcgaaagtct gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg
    16921 gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagcc
    16981 atcgcaaact cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct
    17041 attacggcgg tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct
    17101 caaccgaaat tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc
    17161 cggcaatcca tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac
    17221 agccgtccta aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc
    17281 tgaccaaaag tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc
    17341 gcgacggtta tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc
    17401 tgtcagaaac ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg
    17461 aaccacaggg tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca
    17521 tcgacaaaac agaacacccc ggcccactgc cagaaacggt ggttgcccat ctcgataaaa
    17581 gtcatatttg cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc
    17641 gcgccgatat tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc
    17701 tgatccacca gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttactc
    17761 gcgacattaa cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta
    17821 tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata
    17881 tcttccatac caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca
    17941 aaccggaaga cttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaga
    18001 tgcgcgagat ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc
    18061 aggctccgcc agatttattt acttctgctg cacgaaatta cggtaagccg ccacgacctg
    18121 cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccctc
    18181 ttccatttca tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc
    18241 catccacaga gtgtactcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc
    18301 gtgcgctgct tgttccactt catcaagcaa ggccaggttt tctttcacct cttcattaaa
    18361 ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc
    18421 gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca
    18481 ttgcgccatt gatgtaatta tcgcagcctt accggagaaa aacagcggaa cggcgatggt
    18541 ttagaaataa aaaaatgccc gggaatctca ttcaccgggc attgtgtcgt ttatgcgcag
    18601 cgcgtgcgct tacttattac accgcagtct ggaaaatcac cccatcggct ttttcggtgt
    18661 actgagaaag ctggttgaag ttcagataac gataagtatc aacggcagtt ttatctactt
    18721 gcgccacgta ggtctggtac tcttccggcg tcggcagttt gccaatcagc gccgcaacag
    18781 ccgccagttc cgcagaagcc aggaagacat tcgcgccagt acccagacgg ttcgggaagt
    18841 tacgggtaga ggtggaaacc accgtcgcac cgtctgctac acgcgcctgg ttacccatac
    18901 acagggaaca gccagggatc tcgatacgcg caccactctt accgaagacg ctgtagtagc
    18961 cttcttcagt caactgcgcg gcgtccatac gggttggcgg tgccacccac aggcgggtcg
    19021 gcagttggcc tttgtgcgca tccagcagtt tacccgcagc acggaagtga ccgatgttgg
    19081 tcatgcagga accgataaat acttcgtcga tcttctcacc ctgtaccgca gacagcggac
    19141 gcgcgtcatc cgggtcgttc ggtgcacaga ggattggctc tttaatatcc gccagatcga
    19201 tgtcgatcac tgccgcgtat tcagcatctg catcggcttc cagcagctca ggattcgcca
    19261 gccatttttc catgccctga atacgacgtt ccagggtgcg acgatcgccg taaccttccg
    19321 cgatcatcca cttcagcagg acgatgttag agttcaggta ttcgatgatc ggttctttgt
    19381 tcagcttgat ggtacaacca gcggcagaac gctcggcgga cgcatcggtc agttcaaatg
    19441 cctgctcaac tttcagatcc ggcagacctt caatttccag gatgcggcca gagaagatgt
    19501 ttttcttgcc tttcttctca acggtcagca gaccttgttt gatcgcatac agcggaatgg
    19561 catgtaccag atcgcgcagg gtgatgcccg gctgcatttt gcctttgaag cgcaccagaa
    19621 cggattccgg catatccagc ggcattacgc ctgttgcggc tgcaaatgcc accagaccag
    19681 aacccgccgg gaaagagata ccgatcggga aacgggtatg ggaatcacca ccggtaccga
    19741 cggtatccgg cagcagcata cggttcagcc aggagtgaat gacgccgtca cccggacgca
    19801 gcgacacacc gccacggttc ataatgaagt ccggcagcgt gtggtgcgtg ttcacgtcaa
    19861 ctggcttcgg atacgccgcg gtgtggcaga aagactgcat caccaggtca gccgagaagc
    19921 ccaggcacgc caggtctttc agttcatcac gggtcatcgg gccggtggtg tcctgggaac
    19981 ctacagaagt cattttcggt tcacagtacg cgcccggacg aatgcctttc acgccacagg
    20041 cacggcctac cattttttgc gccagcgaga agccgcgatc gctctcagcg acatctttcg
    20101 cctgacggaa cacatcactg tgcggcagac caagtgcttc acgcgctttg gtggtcaggc
    20161 cacgcccgat aatcagcgga atacggccac cagcacgcac ttcatcaatc agcacgtcgg
    20221 ttttcagttc gaaggtcgcc agcagttcgc cggtttcgtg gttacgcact tcacctttgt
    20281 acgggtaaac gtcaatcacg tcgcccatgt tcaggttaga gacgtcgact tcgattggca
    20341 gtgcacccgc gtcttccatc gtgttaaaga agatgggtgc aattttaccg ccgaggcaca
    20401 aaccaccgcc gcgtttgttc ggcacatgtg gaatatcatc gcccataaac cacagaacgg
    20461 agttagtggc ggatttacgc gaagaacccg tacccacaac gtcaccgacg tacgccagcg
    20521 ggaaaccttt ctgttgcaga gcttcgattt gcttgatcgg accaacaaca ccaggctggt
    20581 ctggctcaat accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg
    20641 ggcgtgacca cgcatccggt gccggagaaa ggtcatcggt gttagtttcg ccagtgactt
    20701 tgaagacggt aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat
    20761 ccgcccagga ctgcataacc tgcttcgcat attcgttgcc tgctttcgct ttctcttcta
    20821 cgtcatagaa gttatcgaac atcagcagcg tgtgagaaag tgctttggca gcaataggtg
    20881 ccagtttggc atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatgg
    20941 tgcccagcag ttcgatggct ttttccggag tcagcagagg ggatttggct tcgcctttcg
    21001 cgatagcagc caggaagcct gctttgacat aggcggcttc atcgacgcct gggggaacac
    21061 ggttggttaa cagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct
    21121 ctacaagtgc ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac
    21181 gctcagctac gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca
    21241 tagtgcggca ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc
    21301 cagtaccgca acgggcagaa tagcaggatt ttgacgatgt gttaatctgt ttacaaaaaa
    21361 gcaacataaa aaagtggctg aatcgttaag gatggtctag agattgtttc cctccgtaaa
    21421 aacttcgtta attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa
    21481 atttaaacta ccgcctcttt atactcggat tcacagcacc tgcgggtggc agttcgccga
    21541 ccattgcgat ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt
    21601 gctggcgcta atttgcagtg ccgggctttg tgccgcctct accgggctat atataaaaag
    21661 ccgcacagtg gaagcgcctg tggaaccgca atcgacacaa cagactgcgc ctgacatcac
    21721 cgcagttacg cttcctgcaa cggtttccgc accgcccgta acgcccgccg tcgtcaaatc
    21781 cgcattcagc actgcacaaa tcgatcaatg ggttgcgcct gtcgcgctgt accccgattc
    21841 tctgctttca caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtaca
    21901 atggtcgcac gataatccac ttaaacaagg cgatgctgct attcaggcgg tatctgacca
    21961 gccgtgggac gccagcgtta aatcactggt ggcctttcca caattgatgg cattgatggg
    22021 cgaaaacccg caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt
    22081 gatggactcg gtacagcgat tgcggcaact ggcgcaacaa accggctcgc tgaagtcatc
    22141 aaccgaacag aaagttatta ccacaacgaa gaaagctgta ccggtaacac agacagtcac
    22201 ggctcccgtc ataccatcca ataccgtttc aactgccagc cccgtcatta cagagcctgc
    22261 aacaaccgtc atttccattg agcccgccaa tcctgatgtg gtctatattc ccaactacaa
    22321 cccaaccgtg gtttacggga actgggccaa tactgcgtat ccgccggttt atctgccacc
    22381 accagccgga gaaccgttta ttgacagctt tgtgcgcgga ttcggctata gcatgggtgt
    22441 tgctaccacg tacgcactat tcagcagcat cgactgggac gacgacgatc atgaccatca
    22501 tcatcatgac gatgatgatt atcatcacca cgatggcggt catcgtgacg gtaatggctg
    22561 gcaacacaac ggcgacaaca tcaatatcga cgtcaacaat ttcaaccgta tcaccggtga
    22621 gcatcttact gataagaata tggcatggcg gcacaatcca aactaccgta atggtgtgcc
    22681 ctatcatgat caggatatgg caaagcggtt tcatcaaacc gatgtcaacg gcggaatgag
    22741 cgccacgcag ctacctgctc caacacgcga cagccagcgt caggcggcag caagtcagtt
    22801 tcagcaacga acacacgccg cccccgtcat tacacgagat acccaacgtc aggcagcggc
    22861 acagcggttt aatgaagctg aacactatgg gagctatgac gacttccgcg acttcagccg
    22921 tcgccaaccc ctgacccagc aacaaaagga cgccgctcgt cagcgttatc agtccgcctc
    22981 gcctgagcag cgccaggcgg ttcgcgagaa aatgcagact aacccacaga accagcagcg
    23041 aagagaggca gcgcgtgagc gtattcagtc tgcaactccc gaacagcgcc aggtatttaa
    23101 ggaaaaagta cagcagcgcc cactgaacca acagcaacgt gataacgccc gccagcgtat
    23161 acaatcggca tcacctgaac aacgtcaggt ttttcgggaa aaagttcagg agagccgccc
    23221 acaacgtcta aacgacagta accatactgt caggctgaat aacgagcaac ggtcggcagt
    23281 acgcgaacgt ctctctgagc gcggagcaag gcgacaggaa aggtaaattg cagacgtaaa
    23341 aaaagcggcg tggttagccg cttttttaat tgccggatgt tccggcaaac gaacaattac
    23401 ttcttcttcg ctttcgggtt cggcaggtcg gtaatgctac cttcgaacac ttctgccgcc
    23461 aggcccacag actcgtgcag agtcgggtgc gcgtggatgg tcagtgcgat gtcttcagca
    23521 tcacaaccca tttcgattgc caggccgatt tcacccagca gctcgccgcc gttggtaccg
    23581 acaatcgcac caccgatcac acggtgagat tctttgtcga aaatcagctt ggtcataccg
    23641 tctgcgcagt cggaagcgat agcacgacca gaagcagccc acgggaaggt ggcggtttca
    23701 tagctgatgc ctttctcttt cgcttctttc tcagtcagac ctacccatgc aacttctggt
    23761 tcggtatagg cgatggacgg gataactttc ggatcgaagt agtgtttctt accggcgata
    23821 acttcagcgg caacgtgacc ttcgtgaaca cctttgtgtg ccagcatcgg ctgaccgacg
    23881 atatcgccga tagcaaagat gtgcggtacg ttggtacgca gctgtttgtc aacgcggatg
    23941 aaaccacggt cgtccacttc cacgccagct ttgcctgcgt cgaggttttt accgttcggc
    24001 acacgaccaa tcgctaccag cacggcgtcg taacgctgcg gttcagcggg tgcttttttg
    24061 ccttccatcg tcacataaat accgtcttct ttcgcttcaa cggcggtaac tttggtttcc
    24121 agcatcaggt tgaatttctt gctgatacgc ttggtgaaga ctttaacgat gtctttgtca
    24181 gctgccggga taacctggtc gaacatttca accacgtcaa tctgtgaacc cagcgcgtgg
    24241 tatacggtgc ccatttccag accgatgata ccgccaccca ttaccagcag gcgttctggt
    24301 acttctttca gttccagcgc gtcagtggag tcccagatac gcggatcttc atgcggaata
    24361 aacggcagtt ggatcgggcg agaacccgct gcaatgatcg cgttgtcgaa gttgatcacg
    24421 gttttaccgt tctcaccttc aacttccagg gtgttagccc cggtaaattt acccagaccg
    24481 ttgaccactt tgactttgcg gcctttcgcc ataccagcca gaccaccggt cagctgattg
    24541 atcactttct ctttccaggt acgaatcttg tcaatatcgg ttttcggttc gccgaagacg
    24601 ataccgtgtt cagccagcgc tttggcttct tcgataactt ttgctacgtg cagcagtgct
    24661 ttagaaggga tacagccgac gttcaggcaa acaccgccaa gggtgttgta acgttctacg
    24721 attacggttt ccagacctaa atcagcgcaa cggaaggcag cggagtaacc tgcggggcct
    24781 gccccaagta ccacgacctg agttttgatt tcagtactca tcatgacctc tatatattta
    24841 tctccggcgg tcatacccgt cgtctttcag gccgcagcgg cgttagcttc acgccctcac
    24901 cccagtcact tacttatgta agctcctggg gattctggcg cttgctacct tgctgcaccc
    24961 agaaatccat agggtatgtg aagtaatacc ctaaccacca ccgggtcgtt ctatccgtcg
    25021 gtattttaca aaattgttaa caatttttaa acaacaaacg gcaaccgatt tgtctattcg
    25081 ctaataacct caatacattc atgagattac cagaaaaaag ccggccgttg ggccggctct
    25141 tttacttaca tcaccagacg gcgaatgtca gacagcgtgt tgttaatgat ggtaatgaaa
    25201 cgggcaccat cagcaccgtc gatcacgcgg tggtcgaagg agagagaaat cggcagcatc
    25261 agacgcggca cgaactcttt accattccac accggctcca tcgcggactt ggaaacgccg
    25321 aggatagcca cttccggcgc gttcacaatc ggcgcgaagt gggtagtacc caggccgccg
    25381 atgctggaga tggtgaagca accgccctgc atttcgcccg cagtcagctt accgtcacgc
    25441 gctttcttag aaatagtcat cagctcgcga gacagctcga tgatgccttt cttgttgacg
    25501 tctttgaata ccggaacaac cagaccgttc ggggtatcca ccgccacacc gatgttgatg
    25561 tatttcttca gggtcagacg ctgaccgtct tccgacaacg aactgttgaa gcgaggcatc
    25621 tgctcaagag ctgcagcaac ggctttcatg atgaagacaa ccggggtgat cttcacatcc
    25681 agcttacgtt tcgccgcttc ttcgttctgc tgtttacgga acgcttccag ctcggtgata
    25741 tcggttttgt cgaagtgagt aacatgcggg atcatcaccc agttacggct caggttcgca
    25801 ccagaaattt tctggatacg gcccagttcc acttcttcga tttcaccaaa cttgctgaag
    25861 tccaccttcg gccacggcag catgcctggg ataccgccgc cagtcgccgc cggagctgct
    25921 tctgcacgtt tgatagcttc tttcacgtaa gcctgaacgt cttcgcgcag gatacgacct
    25981 ttacggccag tgcccttcac tttcgccagg ttaacgccaa actcgcgtgc cagacggcgg
    26041 atcagcggag tcgcgtgaac gtaagcgtcg ttttcagcaa attcagattt gccttcggct
    26101 ttcgcagccg gagctgctgc cggagcttca gcttttgctg ccggagccgg cgctgccgct
    26161 tcctgtttcg ccggagccgc cgcaggcgct gcgccttcaa cttcgaagat cataatcagc
    26221 gagccagttt tcactttatc gccaacgttg actttcagtt ccttcacgac gcctgcaaac
    26281 ggagccggaa cttccataga agctttgtcg ccttctacgg taatcagtga ctgttcagcg
    26341 gcaactttgt cgcccacttt caccatcact tcggtcactt caacttcgtc accgccgata
    26401 tccggaacgt taacttcttt cacgccagcc gctggtgcag gggccgctgc cggagccgct
    26461 tcctgtttag ccgccggagc tgccgcgcct gcttcacccg cgacttcgaa gaccataatc
    26521 agcgagccgg tagacacttt gtcacccacg ttcactttga tctctttcac ggtgccagca
    26581 aacggagccg gaacttccat agaagcttta tcgccttcta cggtgatcag cgactgttca
    26641 gcttcaactt tatcgccaac tttcaccagg atttcggtca cttcaacttc gtcgctgccg
    26701 atatccggaa cgttaacgtc ttttgccgcc gcagccgcgg gtgctgctgc cggagctgct
    26761 tctttcttct cttctgcctg agcaggtgca gcgtctgctg caccgtcggc ggaatcgaaa
    26821 atcataatca gtgcgccggt ctgggtttta tcgccaacag agactttgat ctctttaacg
    26881 atacccgcct gcggagacgg aacttccata gaggctttgt cgccttctac ggtgatcagc
    26941 gactgttcgg cttcaacttt gtcgcccact ttgaccagga tctcggtgat ttcaacttca
    27001 tcagccccga tgtccggtac tttgatttcg atagccatta ttcttttacc tcttacgcca
    27061 gacgcgggtt aactttatct gcatcgatgt tgaatttggc gattgcgtca gcaaccactt
    27121 tcttatcgat ttcgccacgt ttagccagtt cgcccagcgc cgcaaccacg acataagaag
    27181 catcaacttc gaagtggtga cgcaggttct cacggctgtc ggaacgaccg aagccatcag
    27241 tacccagtac gcggtagtcg tcagccggta cgtaagtacg gacctgctca gcgaacagtt
    27301 tcatatagtc ggtagatgcc actgccggag cgtcgttcat cacctgagcg atatacggaa
    27361 cgcgcggagt ttccagcgga tgaagcatgt tccagcgttc acaatcctga ccatcacgcg
    27421 ccagctcggt gaaggaggtc acgctataaa cgtcagaacc tacgccgtaa tctttcgcca
    27481 ggatctctgc cgcttcacgg acgtgacgca ggatagaacc ggagcccagc agctgaactt
    27541 tacctttgct accttcaatg gtttcgagtt tgtagatacc tttacggata ccttcctcag
    27601 caccttccgg cattgccggc atgtggtagt tttcgttcag cgtagtgatg tagtagtaaa
    27661 cgttctcttg tttttcaccg tacatacgct ccagaccgtc atgcatgatg acagcaactt
    27721 cgtaagcgta agccgggtcg taagagatac agttcgggat agtcagcgac tgaatgtggc
    27781 tgtgaccatc ttcgtgctgc agaccttcac cgttcagggt ggtacgaccg gaagtaccgc
    27841 cgatcaggaa gccacgcgct tgctggtcgc cagccgccca gcacagatcg ccgatacgct
    27901 ggaagccgaa catcgagtaa tagatgtaga acgggatcat cggcagattg ttggtgctgt
    27961 aagaggtcgc cgctgccagc caggaacaac ctgcgcccag ctcgttgatc ccttcctgca
    28021 gaatctgacc tttctcgtct tctttatagt aagcaacctg ctcgcggtcc tgcggggtgt
    28081 actgctgacc gttcgggctg taaataccaa tctgacggaa cagaccttcc ataccgaaag
    28141 tacgcgcttc gtcggcgatg atcggtacca gacgatcttt gatcgacttg ttcttcagca
    28201 ttacgttcag ggcacgaacg aaagcgatag tggtagagat ctctttgctc tgctcttcca
    28261 gcagcgcgcc gaagtcttgc aggctcggca gctcaagctt ctcggtgaag ttcggctgac
    28321 ggcttggcag ataaccgtgc agtttctgac gctgtgcatg cagataggta tgctcttcag
    28381 aaccttccgg gaaggtgatg tacggcagtt tttcgatatc tgcatcagac accggcacat
    28441 tgaaacggtc gcggatgtga cgcacgccgt ccatgttcat tttcttaacc tggtgcgcga
    28501 tgtttttacc ttcagccgcg tcgcccatgc cgtaaccttt aatggtatga gcaaggatta
    28561 ctgtcgcttt gcctttggtt tcctgcgctt tcttgaatgc agcgtagatt ttcttcggat
    28621 cgtgaccacc acggttcagt gcccagatct gctcgtcagt ccagtctgca accagtgctg
    28681 cggtttcagg atatttaccg aagaagtgtt cacgaacgta cgcaccatct ttcgatttga
    28741 aggtctggta gtcgccgtca acggtttcgt tcatcagctg gatcagttta ccgctggtat
    28801 ctttacgcag cagttcatcc caacggctac cccacatcac tttgatcacg ttccagccag
    28861 caccttcgaa gatgccttcc agttcgttga tgatcttgcc gttaccggtg accgggccgt
    28921 caagacgctg caggttacag ttgataacga agaccaggtt atccagtttt tcacgggtag
    28981 cgatggtgat cgcacctttg gattccggtt cgtccatttc accgtcgccg aggaacgcgt
    29041 aaacggtctg tttagaggta tctttcaggc cacggtgttc cagatatttc aggaatttag
    29101 cctggtaaat agcaccaatc ggccccagac ccatagatac ggtcgggaac tgccagaatt
    29161 ccggcatcag tttcgggtgc ggataggaag agaggccatt gccgtgaact tcctgacgga
    29221 agttatccag ctgctcctga gtcagacgac cttccaggaa agcacgagcg tacacgcccg
    29281 gggagatgtg gccctggaag taaaccaggt cgccgccatc ctgctcgttg cgtgcacgga
    29341 agaagtggtt aaagcacaca tcataaatgg ttgcggaaga ctggaaggac gccatatggc
    29401 cgcccagttc gaggtctttt ttcgacgcac gcagcaccgt catgatggcg ttccagcgga
    29461 tagctgaacg aatacggcgt tccagttcca gattacccgg atactccggt tgttcttcaa
    29521 cggggatggt gttgatgtag ttgctgatac ctgtgcctgc ggctacgttt acaccgcctt
    29581 tgcgggcttc agcaagcagt tggtcgatca gatactgagc acgctcaaca ccttcttcac
    29641 ggatgaccga ttcgatcgcc tggagccagt cgcgagtttc gatcggatcc acgtcatttg
    29701 ggaaacgttc tgacatgggt tattccttat ctatctaata acgttgagtt ttctggaacc
    29761 tgtcccattg aactctcgcc ggaaagctca ataagacagg ttctacgttt agttgccgcg
    29821 cttttatatg cgcttgattt acaacatctt ctggataatt tttaccagaa aaatcactaa
    29881 ttctttcgtt gctccagacg acgcagagaa cgctcacggc ggctctcttc acgacttctg
    29941 tcgagcaaaa tttcttcgat aaaggccaga tggcgatgcg atgcttcgcg cgcttcttcc
    30001 ggcttaccgg ccataatcgc ttcaaatatg cgggtgcggt gactactcac cagcggcagc
    30061 atctcgcgac gcgaatagag caattcgaag ttctggcgga cattctgggc caacatcggc
    30121 tccatacacc ttagcagatg aagcagaacc acattgtggg ccgcttcggt gacggcaatc
    30181 tgatactgga gtacggcgtt tgattccgcg tccagatcgc cagactgctg cgccagctct
    30241 atggcgtggt ggagttcacg gatgcgttcc ttgtcttcat cggtactacg cagcgcggcg
    30301 taataagcgg cgataccttc cagggcgtgt cgtgtttcga gcaagtcata ctgtgactca
    30361 ggatggtcgg agagcagctc caccagcgga tcgctgaagc tttgccatag gctgctctgg
    30421 acaaaagtgc cgccaccctg gcgacgaagc aacaagccct tcgcttcgag acgttgaatc
    30481 gcctcacgca aggagggacg ggagacgtca aactgttttg ccagttcgcg ttccggtggg
    30541 agtttttcgc ccgggcggag agtgccttcg aggatcaaaa actccagttg ctgctcaatc
    30601 acatcggaga gttttggttg gcggattttg ctgtaggcca tgagttcctg tcttaagcca
    30661 cttgccgaag tcaattggtc ttaccaattt catgtctgtg acgctaaagt aacaaagtat
    30721 tcaccttatg tccatacagg ttttgattga aatcatgaaa ctgtgcacat tttaacaact
    30781 tgacatatat aacgtttcaa agttgtaact atgcacaaat gtagacttta cgtaggaaag
    30841 ga
//
LOCUS       DAZFVO020000045        30347 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFVO020000045 DAZFVO020000000
VERSION     DAZFVO020000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 30347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.045"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(91..912)
                     /gene="bacA"
                     /locus_tag="ACHKNB_RS09475"
                     /old_locus_tag="pgaptmp_001895"
     CDS             complement(91..912)
                     /gene="bacA"
                     /locus_tag="ACHKNB_RS09475"
                     /old_locus_tag="pgaptmp_001895"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGU4195421.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(1002..1370)
                     /gene="folB"
                     /locus_tag="ACHKNB_RS09480"
                     /old_locus_tag="pgaptmp_001896"
     CDS             complement(1002..1370)
                     /gene="folB"
                     /locus_tag="ACHKNB_RS09480"
                     /old_locus_tag="pgaptmp_001896"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HGU4195422.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1475..2092
                     /gene="plsY"
                     /locus_tag="ACHKNB_RS09485"
                     /old_locus_tag="pgaptmp_001897"
     CDS             1475..2092
                     /gene="plsY"
                     /locus_tag="ACHKNB_RS09485"
                     /old_locus_tag="pgaptmp_001897"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGU4195423.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2105..3037)
                     /gene="ttdR"
                     /locus_tag="ACHKNB_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /gene_synonym="dan"
     CDS             complement(2105..3037)
                     /gene="ttdR"
                     /locus_tag="ACHKNB_RS09490"
                     /old_locus_tag="pgaptmp_001898"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HGU4195424.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3244..4155
                     /gene="ttdA"
                     /locus_tag="ACHKNB_RS09495"
                     /old_locus_tag="pgaptmp_001899"
     CDS             3244..4155
                     /gene="ttdA"
                     /locus_tag="ACHKNB_RS09495"
                     /old_locus_tag="pgaptmp_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HGU4195425.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4152..4757
                     /gene="ttdB"
                     /locus_tag="ACHKNB_RS09500"
                     /old_locus_tag="pgaptmp_001900"
     CDS             4152..4757
                     /gene="ttdB"
                     /locus_tag="ACHKNB_RS09500"
                     /old_locus_tag="pgaptmp_001900"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HGU4195426.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4805..6268
                     /gene="ttdT"
                     /locus_tag="ACHKNB_RS09505"
                     /old_locus_tag="pgaptmp_001901"
     CDS             4805..6268
                     /gene="ttdT"
                     /locus_tag="ACHKNB_RS09505"
                     /old_locus_tag="pgaptmp_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HGU4195427.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6311..7324)
                     /gene="tsaD"
                     /locus_tag="ACHKNB_RS09510"
                     /old_locus_tag="pgaptmp_001902"
     CDS             complement(6311..7324)
                     /gene="tsaD"
                     /locus_tag="ACHKNB_RS09510"
                     /old_locus_tag="pgaptmp_001902"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGU4195428.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7562..7777
                     /gene="rpsU"
                     /locus_tag="ACHKNB_RS09515"
                     /old_locus_tag="pgaptmp_001903"
     CDS             7562..7777
                     /gene="rpsU"
                     /locus_tag="ACHKNB_RS09515"
                     /old_locus_tag="pgaptmp_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGU4195429.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7888..9633
                     /gene="dnaG"
                     /locus_tag="ACHKNB_RS09520"
                     /old_locus_tag="pgaptmp_001904"
     CDS             7888..9633
                     /gene="dnaG"
                     /locus_tag="ACHKNB_RS09520"
                     /old_locus_tag="pgaptmp_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4195430.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQKPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9828..11669
                     /gene="rpoD"
                     /locus_tag="ACHKNB_RS09525"
                     /old_locus_tag="pgaptmp_001905"
     CDS             9828..11669
                     /gene="rpoD"
                     /locus_tag="ACHKNB_RS09525"
                     /old_locus_tag="pgaptmp_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGU4195431.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSNAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11747..12253)
                     /gene="mug"
                     /locus_tag="ACHKNB_RS09530"
                     /old_locus_tag="pgaptmp_001906"
     CDS             complement(11747..12253)
                     /gene="mug"
                     /locus_tag="ACHKNB_RS09530"
                     /old_locus_tag="pgaptmp_001906"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HGU4195432.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12378..12453
                     /locus_tag="ACHKNB_RS09535"
                     /old_locus_tag="pgaptmp_001907"
     tRNA            12378..12453
                     /locus_tag="ACHKNB_RS09535"
                     /old_locus_tag="pgaptmp_001907"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12411..12413,aa:Ile,seq:cat)
     gene            complement(12507..13271)
                     /gene="nfeF"
                     /locus_tag="ACHKNB_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /gene_synonym="yqjH"
     CDS             complement(12507..13271)
                     /gene="nfeF"
                     /locus_tag="ACHKNB_RS09540"
                     /old_locus_tag="pgaptmp_001908"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HGU4195433.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13548..14171
                     /gene="nfeR"
                     /locus_tag="ACHKNB_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /gene_synonym="yqjI"
     CDS             13548..14171
                     /gene="nfeR"
                     /locus_tag="ACHKNB_RS09545"
                     /old_locus_tag="pgaptmp_001909"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HGU4195434.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14325..15845)
                     /gene="aer"
                     /locus_tag="ACHKNB_RS09550"
                     /old_locus_tag="pgaptmp_001910"
     CDS             complement(14325..15845)
                     /gene="aer"
                     /locus_tag="ACHKNB_RS09550"
                     /old_locus_tag="pgaptmp_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HGU4195435.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16263..17642
                     /gene="ygjG"
                     /locus_tag="ACHKNB_RS09555"
                     /old_locus_tag="pgaptmp_001911"
     CDS             16263..17642
                     /gene="ygjG"
                     /locus_tag="ACHKNB_RS09555"
                     /old_locus_tag="pgaptmp_001911"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HGU4195436.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17684..18016)
                     /locus_tag="ACHKNB_RS09560"
                     /old_locus_tag="pgaptmp_001912"
     CDS             complement(17684..18016)
                     /locus_tag="ACHKNB_RS09560"
                     /old_locus_tag="pgaptmp_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HGU4195437.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18235..19218
                     /gene="ebgR"
                     /locus_tag="ACHKNB_RS09565"
                     /old_locus_tag="pgaptmp_001913"
     CDS             18235..19218
                     /gene="ebgR"
                     /locus_tag="ACHKNB_RS09565"
                     /old_locus_tag="pgaptmp_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HGU4195438.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19402..22494
                     /gene="ebgA"
                     /locus_tag="ACHKNB_RS09570"
                     /old_locus_tag="pgaptmp_001914"
     CDS             19402..22494
                     /gene="ebgA"
                     /locus_tag="ACHKNB_RS09570"
                     /old_locus_tag="pgaptmp_001914"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HGU4195439.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22491..22940
                     /locus_tag="ACHKNB_RS09575"
                     /old_locus_tag="pgaptmp_001915"
     CDS             22491..22940
                     /locus_tag="ACHKNB_RS09575"
                     /old_locus_tag="pgaptmp_001915"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HGU4195440.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            23003..24436
                     /locus_tag="ACHKNB_RS09580"
                     /old_locus_tag="pgaptmp_001916"
     CDS             23003..24436
                     /locus_tag="ACHKNB_RS09580"
                     /old_locus_tag="pgaptmp_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4195441.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24570..25640
                     /gene="ygjJ"
                     /locus_tag="ACHKNB_RS09585"
                     /old_locus_tag="pgaptmp_001917"
     CDS             24570..25640
                     /gene="ygjJ"
                     /locus_tag="ACHKNB_RS09585"
                     /old_locus_tag="pgaptmp_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HGU4195442.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            25657..28008
                     /gene="ygjK"
                     /locus_tag="ACHKNB_RS09590"
                     /old_locus_tag="pgaptmp_001918"
     CDS             25657..28008
                     /gene="ygjK"
                     /locus_tag="ACHKNB_RS09590"
                     /old_locus_tag="pgaptmp_001918"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGU4195443.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            28334..>30347
                     /gene="fadH"
                     /locus_tag="ACHKNB_RS09595"
                     /old_locus_tag="pgaptmp_001919"
     CDS             28334..>30347
                     /gene="fadH"
                     /locus_tag="ACHKNB_RS09595"
                     /old_locus_tag="pgaptmp_001919"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HGU4195444.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         7335 a         8205 c         7858 g         6949 t
ORIGIN      
        1 ccagcaccgg tgcgaacaat cacccccacc ccggccctct ccctacagag cgagggagaa
       61 aaccaggcct gctcccgaac cttttctcaa tcaaaagaac acgacgtaca ccgcagccgc
      121 cacaataaag cgataaatgg cgaacgggat aaacgaaatg cgcttaatca attgcaggaa
      181 ggttttaatc gctatcagcg ccaccacaaa agcggtgata aacccaacgg caaacatcgg
      241 gatatcgccg gttgtcagga agccccagct tttgtagaga tcgagcgccg ttgcgcccat
      301 catcatcggt accgccagca ggaacgagaa ctcggaagca gcgtaacggc tcacgcccat
      361 cagcatccca cctgaaatgg tggccccgga acgggagaaa cccggccaca gcgccagaca
      421 ctggaaacag ccaatcataa acgcctgacg ataggtcata tcatcaagac ccggcgcacg
      481 cggctctttc ggcttcaggc attcggcggc aatcagcaac aaaccgccaa cgaccagtgc
      541 atacatcaca tttatcgggt taaacaatga cttaatcgtg tcgtggaaca acagccccag
      601 taccaccgcc ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac ctttgctttc
      661 tccttcgtgc tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca gacgccgcca
      721 gaacatcact actaccgcca gaattgatcc taactggatc acaacttcaa aggttttcgc
      781 cgtgtcgccc tcgaacccca acaagtgacc gacaataatc atatggcccg tgctggatac
      841 cggcagaaat tctgtcaatc cttcgaccac acccaatatt gccgctatca gcagcgagtg
      901 catatcgctc atcaataaac ccctaaatta ttaaaatgta ccgcttgtcc gaactactgc
      961 gtatgaccag gttataaccg tttggtttaa cagctgtaaa attaattatt ttctttcaga
     1021 ttattgccac gctcaatgat tacgccaaca ttcgccgccc gcgccactgc gcctggcttg
     1081 ctgagtttga tacgcaccca cggcgagttg aagcgtgcta acagcagctc cgccacctct
     1141 tcagccacgc gttccaccag cgcaaaacgc gccccctcga cgtggctgac caccgtttct
     1201 gcaatgtcag cgtaactgag gcaatccgcc acatcatcac ttttcgccgc tttacggtta
     1261 tcccatgcca tttcgatatc gaacactaac ttctgttcga tggtctgttc ccagtcgtaa
     1321 acaccaatag tggtgattac cgaaagttgc tctataaata caatatccat cacgtcctgc
     1381 ctgctttttg gctaaccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca
     1441 tcgttgaaca cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat
     1501 cctcatcgcg tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg
     1561 gctgcccgat ccgcgaacca gcggctccgg caatccaggc gcaaccaatg tgttacgtat
     1621 cggtggcaag ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc
     1681 cgtctggggc gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc
     1741 cgcctgtctt ggacacatct ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc
     1801 taccgctttt ggtgccatcg cacccattgg ctgggatctc accggagtaa tggcgggaac
     1861 ctggttactg accgtgctat tgagcggata ctcgtcgctg ggagcgattg tcagtgcact
     1921 gattgctccg ttttatgtat ggtggtttaa gccacaattc accttcccgg tttcgatgct
     1981 ctcttgcctg atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgtcagga
     2041 gacaaaaatc tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt
     2101 ggatctacat gacctgatag ccttcatcgg gcttgcccag ccgttgctgg caccatgccg
     2161 ccagaaattc cacgcagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag
     2221 cccagatatt ggcgctttgt gcatactccg gcaatacttg caccagtttg ccactctcca
     2281 gaaacggcaa cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt
     2341 gcaacactat ctcgccgcta ttggaggaaa gatgcccgct aaccttcacc gattttttct
     2401 cctgaccgtt ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccagac
     2461 agtcatgacg acttaattcc tgtaatgatt gtggctgcgg atatttttgc agatactcag
     2521 gtgctgcgca taatattctt ttatttttcg ttaacagatg cgcaatataa taatcaggaa
     2581 tttcgtcatt aatacgaata tccagatcaa tattatcctg cactaaatca atttgccgat
     2641 cgaacaattc aaaatgcacc tgtaactcag gataattgcg cataagttcg gtaatggctg
     2701 gcgcaatatg gctgcgccca aaaccaaaac tacagccaat acgaatcatc ccctccgggc
     2761 gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg
     2821 catgttcgta acaacgttgg ccactttcag tcagcgccac gccgcgagcg gagcggttca
     2881 gcagcgttgt tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg
     2941 tctgcccaag cgtcgccgct gcggcgctaa aactcccgct gtggacgatt tctaccagta
     3001 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
     3061 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
     3121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
     3181 acatttttag tggtagtttg tggcggtgaa tttttccaga caaatacaaa aactggagtt
     3241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
     3301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
     3361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
     3421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
     3481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
     3541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
     3601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
     3661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacgctacct
     3721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtagtgaa attcgtcttc
     3781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
     3841 gccacctcgg tggaaaccgc cgccgtgctc tcgcgaaaag ccattttgcg cccgattggc
     3901 agccgccatc ccaatccaaa agcggcagag ctggagctac gcctggaaga aggactcaac
     3961 cgtctgggaa ttggcccaca agggctgact ggcaacagtt cagtgatggg cgtgcatatc
     4021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tttctacagg ctgctgggcg
     4081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     4141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     4201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     4261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     4321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     4381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     4441 aactggtggt tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca
     4501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag
     4561 agattgaaga agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg
     4621 tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg
     4681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     4741 tccattacat caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa
     4801 cacgatgaaa ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat
     4861 tattgctcta cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt
     4921 ttttactggc gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat
     4981 ggtgggtatc tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct
     5041 cgcccagcca ggctttaaat tcactgcaaa atccctctcg tgggcggttt ccggtttttc
     5101 taattcggtt atctggctga ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac
     5161 cgggcttgga cgccgcattg cgctgtttct ggtgaaaaag atgggacatc gcacgctgtt
     5221 tctcggctat gcggtgatgt tctccgagct gatcctcgca cctgtaacac cgtccaactc
     5281 ggcgcgtggt gcggggatta tctatcccat catccgtaac ctgccaccgc tctatcaatc
     5341 acaaccaaac gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt
     5401 tgccgactgc gtgaccagcg ccattttctt gacggcgatg gcaccaaact tgctgttaat
     5461 tggactgatg aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat
     5521 gttgccgctc agtattttgc tggtgctgct ggttccctgg ctggcttatg tgctgtaccc
     5581 gccggtactg aagtctggcg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat
     5641 gggcccgctc tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct
     5701 gtggattttc ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcact
     5761 gatgctgctt ctgcgcatta tcagttggga cgacattgtc agtaataaag cggcgtggaa
     5821 cgttttcttc tggctggcct cgcttatcac cctcgctacc ggactcaaca acactggttt
     5881 tattagctgg tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatggt
     5941 gatggtggcg ttgattgtgg tgttttatct gctgcgctac tttttcgcca gcgccacggc
     6001 gtatacctcc gccctcgcac cgatgatgat tgccgctgcg ctggcgatgc cggaaatccc
     6061 gctgccggtg ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc
     6121 atacgccacc ggacccagtc cgatttacta cggtagtggt tatctgccaa cggtggatta
     6181 ctggcgactg ggggcgattt ttggactgat attcctcgtg ttgctggtga ttaccggctt
     6241 gctgtggatg cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca
     6301 ccggatagtt ttacgcagcc ggtaactctg ccagtggcca gcgcggacgc acgctaacgc
     6361 cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc
     6421 cgttatcagt acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca
     6481 tttccgccag cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc
     6541 gcttaaagcc cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg
     6601 catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa
     6661 tcgtgttcgc cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg
     6721 tcatcggacg cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta
     6781 acggtccgcc aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg
     6841 cggcatcatc gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta
     6901 actgcgtatg accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt
     6961 ccagcatcgg cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc
     7021 aggcaaacgc cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag
     7081 ggcctgcggt ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg
     7141 ccgcctggat caacggtacg gttttgcgca cgtgatcgcg ggaggccagt tcaggcacga
     7201 cgccgccgta gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt
     7261 tttcatcgtc gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac
     7321 gcatgacttg ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt
     7381 aaaactttgt tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc
     7441 tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     7501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     7561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     7621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     7681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     7741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     7801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     7861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     7921 gatctgctgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     7981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     8041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     8101 gacttcctga tgaactacga caagctcgag ttcgttgaaa cggtcgaaga gctggcagca
     8161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     8221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     8281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     8341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     8401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     8461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     8521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     8581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     8641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     8701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     8761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     8821 cgtgcaggcc gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     8881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     8941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccgct ttccgcattt
     9001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     9061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     9121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     9181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     9241 cgtatactta tagggttgct ggtgcaaaaa ccagaattag cgacgttggt cccgccgctt
     9301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     9361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     9421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     9481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     9541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     9601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     9661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     9721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     9781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     9841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     9901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     9961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
    10021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
    10081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
    10141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
    10201 acgggatcaa ccaagttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
    10261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
    10321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
    10381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
    10441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
    10501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt aacgctgccg
    10561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
    10621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
    10681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
    10741 tgtttaccgg caatgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
    10801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
    10861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
    10921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
    10981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
    11041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
    11101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
    11161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
    11221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
    11281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
    11341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
    11401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
    11461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
    11521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
    11581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
    11641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
    11701 ccggcactag tccctctgca caaacgccac cttttcggtg gcgtttttat cgcccacgca
    11761 ctaccagcgc ctggtccagc tcgcgatatg cttcaaccag tttctctagt gaaacgcgac
    11821 ttaaaccgct gggatttggc agcacccaaa tctgcgtcga accaatggtg agcgtttgtt
    11881 tcccccactg tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg
    11941 ccaacgcctg cggctgataa tcttcaattt tttcaatcag cttacgccca cctgcgtgca
    12001 gctcctgctt tgaaacttca ttggcttgca ccgttggacg gtctaccagt ttggtgacgc
    12061 cacaacgata atccagcaga tgctgtgcct cctgcggctt caactgacgg tcggtaaacc
    12121 cggcctgata tatcaccttc cagaagcgat ttgctggatg agcaaaggga aaaccagtcc
    12181 cggcggatga aagcccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa
    12241 tatcctcaac catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa
    12301 tacattgttt ataaaaacag caggcgcacg gtaatggctg gattgcgaca cggagttact
    12361 ttataatccg ctaccatggc cccttagctc agtggttaga gcaggcgact cataatcgct
    12421 tggtcgctgg ttcaagtcca gcaggggcca ccagatatag caaaggctga cgagaaatcg
    12481 tcagcctttt tctttttata tatcagttac tttgcgtgcc agtaagccgc tgcacgtacc
    12541 cgctgtgggt catactgttc cgcttcaaag cggcggctta aattcttaac gactttacct
    12601 tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca
    12661 tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga
    12721 taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca
    12781 agtttgctca acgtttccag gcggcggcgc agtgcgggca ttccggattc atcgcagaca
    12841 tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc tgccaccgta
    12901 agtttatcgc ccggttgcgc ctgcatcgcc cagccgctgg cgaccccacc gtcgtgaata
    12961 aagaaatcga tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac
    13021 ggtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag
    13081 tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgatgtaaaa
    13141 ccgtccagcg cctcgccgcc gaggacaatg cgctgaaaac cggcgctgat gcgctcaacg
    13201 cgtaacacag tcagttcacg gaagcgcaga tcattgcgaa cgcgctgcgg gtagcggggg
    13261 gagttattca ttgttatcgc cttcgtgata gcaatcagat atatctaaat aattttcgta
    13321 aatgataatg attgttaatc atgataaatg caagcgattt gtagaactga tatatctgaa
    13381 gtatgtgaag gcattcagtg ccatagcaat gctgccggat gcggcgtgaa cgtcttatcc
    13441 ggcctggatg tgaggtgctt ccacgtttaa acatcatttt tgtacttgca gtaatgctca
    13501 catcagatat aaattagata tatctaatca agcaaaagga ggctgatatg agccatcatc
    13561 acgaagggtg ttgtaaacat gaaggccagc cacgccatga gggctgctgc aaaggtgaga
    13621 agtcagaaca cgagcactgc ggacacggtc accagcatga acacggtcaa tgctgcggtg
    13681 gtcgccacgg tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc
    13741 tggtgattct ggatattctc tcgcgcgatg acagccacgg ttacgaattg attaaagcga
    13801 ttgagaacct aacccagggg aattacaccc caagcccggg cgtcatctac ccaacgctgg
    13861 attttctgca ggagcagtcg ctgattacca tccgcgaaga ggaaggtggt aagaagcaga
    13921 ttgcgctgac cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga
    13981 ttgaagaacg catcaaagcg cgttgcgttg gcgcggcgct gcgccagaac ccgcaaatga
    14041 agcgggcgct ggataatttt aaagcggtgc tggatttaca cgtcaaccag agcgatatca
    14101 gtgatgcaca aataaaaaag atcattgcgg tgatcgaccg cgccgctttt gatattacgc
    14161 aactggatta atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg cgacgcttga
    14221 cgcgccttat ccggcctaca cccgctacac accccgcagg cctgataaga tgcgccagca
    14281 tcgcatcagg cattgtgctc caaccgccgg atccggcata ccgattaatg cagcaccgtc
    14341 accgcgtctt ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg cgcactctct
    14401 tccaccagct cggcattttt ctgggtgatc aggttaagct catccactgc acgggtcagg
    14461 ctggaaagcc catcggcctg ttccagcgtt gaatggctaa tctgggcgat caactgggtg
    14521 acgtttttca cctgtgccac aatatcttcc atcgtccgtc cggcggcgtg tacctgctgc
    14581 gaaccggatt gcaccttatc agcactggca tcaatcagct tgcgaatatc gttggcagca
    14641 ttagcgctgc ggctggctaa atgacgcact tcccctgcca ccactgcaaa acctttgccc
    14701 tgttcgccgg cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt ggtctgaaac
    14761 gcaatatcgt taatcagcga agtaatggtg ccaatgcgct gggtactgtc ggcgatatcg
    14821 tccattgtct tgatcaccgt ggtcatcgcc tccccaccct gcactgccgc attactggcg
    14881 gtgatagaaa gtttatcggc agccgacgcc gtggcagagt tctgtttcac cgacgccgcc
    14941 atttggttca tggtcgccac cgtttgctga acgttatcaa ctgtctgctg ggtatgttcg
    15001 ttcagttcat cggtgccttt cgccagcgtc tcactgccat ttctgacact ggacacctgg
    15061 cttgagacat cgttaattag ccaacggcac atcaggccaa gctgccctac cgcgcgtaat
    15121 gtcagcccca gctcatcgct gcgattcagg tgctcaacac tattacgctc tccggtcgcc
    15181 accttcagtg cctgacgggc gacattttct atcgggcgca caatctgcca ttcaaaacaa
    15241 gcgcttgcca acaataccac taacacacag aggaaatacg tcaccaccgg ggcagcaaca
    15301 aaccaaagca tggccgccag caagataaac atcagggtca tcactccacg cgcccgccag
    15361 cgaagcggta atgaaggcag tttacccagc cagcctttac gcaccaccag gcctttatga
    15421 atacgcttac tggtacgtcc ggcgttcagc gctttgtaca gcggctccac cgccgcaatc
    15481 tcttcatccg tcgcccgggt acgaatcgac atatagccac tgatttttcc ctcgcgcacc
    15541 atcggtaccg cattggcccg cacccaataa tggtcaccat ttttgcggcg atttttcacg
    15601 atgccgctcc agggctcccc ttttttcagg gtgaaccaca tatccgcaaa cgccgctttt
    15661 ggcatatccg ggtgacgcac catgttgtgc ggctgccctt gtaactcttg caaggtaaag
    15721 ccgctcacct gcacaaaagt gtcattagca tgagtgatat agctttgcag atcggtagtg
    15781 gacatcagag tggtatcgtc cgccagcggg gtattttgct gggtgacata cggatgagaa
    15841 gacatgttcg cgtcctgtgc aggttatatg gttgttaact tcttgtcaga gtttatgtcg
    15901 gccccgctgc ggttatcttt aaccgattaa tttgatttag atcgcaattt gcgattaaag
    15961 cacaaatcta attctttgat ttaaaatact ttcactctgt tactatacga aaacgttaat
    16021 tatcttgccc aaaaatcagg caattattgc cctgaaaacg tgcatttgcg cagcaatcat
    16081 caaatccata cccgacaaaa accgtgcaaa ataacaacaa atgttaacag atagcattaa
    16141 atattgcaca aatgataacc gaatttgtgt ttatcccgat tttcgcgatc gcagccggag
    16201 tggcgcaatc cctgcaatac ttaaatcggt atcatgtgat acgcgagcct ccggagcata
    16261 ttttgaacag gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc
    16321 tcattgagaa gcgaacgctg gatcatgagg agatgaaagc acttaaccga gaggtgattg
    16381 aatacttcaa agagcatgtc aatccggggt ttttagagta tcgcaaatct gttaccgccg
    16441 gcggggatta cggagccgta gagtggcaag cgggaggttt aaatacgctt gtcgacaccc
    16501 agggacagga gtttatcgac tgcctgggag gttttggaat tttcaacgtg gggcaccgta
    16561 atccagttgt ggtttccgcc gtacagaatc aacttgcgaa acaaccgctg cacagccagg
    16621 agctgctcga tccgttacgg gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta
    16681 aactgaaata cagcttcttc tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc
    16741 tggcgaaagc ttaccagtca ccgcgcggca agtttacttt tattgccacc agcggcgcgt
    16801 tccacggtaa atcacttggc gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt
    16861 ttatgccact gctgccgggc ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca
    16921 cggctcttaa cgagtgcaaa aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga
    16981 ttcagggtga aggtggcgta attctgccgc cgccgggcta tctcaccgcc gtacgtaagc
    17041 tatgcgatga gttcggcgca ctgatgatcc tcgatgaagt acaaacgggc atggggcgca
    17101 cgggcaagat gttcgcctgc gagcatgaga acgtacagcc ggatatcctc tgccttgcca
    17161 aagcgctcgg cggcggcgtg atgccgattg gcgcgaccat cgccactgaa gaggtgtttt
    17221 cagttctgtt cgacaaccca ttcctgcata ccaccacctt tggcggcaac ccgctggcct
    17281 gtgcggcggc gctggcgacc atcaatgtgt tgctggagca gaacttaccg gctcaggctg
    17341 agcaaaaagg cgatatgttg ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc
    17401 tggtacagga agcgcgtggt aaagggatgt tgatggcaat tgagtttgtt gataacgaaa
    17461 tcggctataa ctttgccagc gagatgttcc gccagcgcgt actggtggcc ggaacgctca
    17521 ataacgccaa aacgatccgc attgaaccgc cactgacact gaccattgaa cagtgtgaac
    17581 tggtgatcaa agcggcgcgt aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt
    17641 aatatcaaat cggatggcga tacgacgtcg ccatccgatc tttttatacg acgcgtacgc
    17701 ccgcaggcat catccgctcc ggcgttaaca acacgctttc actgccgtca tcggtttccg
    17761 cacacaacag catgcattcc gacgtttcac cacgcatttt cgctttttgc agattgcata
    17821 agaccaccac cgttttcccc atcagctctt cttcgctgta gtacggcacc aggctggtca
    17881 cggtttgcag tgttttttcc cccacatcaa cctgtacgat gtacagcttg tcggcgtttt
    17941 catggcgttt cacttccaca atctttccga cgcgcatttc cagacgtgca aaatcagcgt
    18001 aagccacggt ttccattgtt tcctcccttc gggtaaaatt ttactaaact atagcaaaag
    18061 tttttctcaa tcctgtaggc taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg
    18121 ctgtgttacc gttacagcgt caaagaaacg cgctttattt actgaaaaca ggtgacccga
    18181 taagcacttc ctctacaatg ggggcgcaca tcagggaaag taaaaaaggt aaacatggca
    18241 acactaaaag acatcgcaat cgaagctggc gtatccctgg cgacagtatc cagggtctta
    18301 aatgacgatc cgacattgaa tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc
    18361 gaaaagctgg agtacaagac cagtagtgcc cgtaaactcc agacaggtgc agtcaaccaa
    18421 caccatattc tggctatcta cagctaccaa caggagctgg agatcaacga tccttactat
    18481 ctggcgatcc gccacggcat tgaaactcag tgcgaaaagc tgggcattga gctcaccaac
    18541 tgttatgaac acagcggatt accagacatt aaaaacgtca ccggtatttt aattgtcggc
    18601 aaacccacgc ccgccctgcg cgccgccgcc tgtgcgttga ccgacaatat ctgttttatc
    18661 gactttcacg aacccggcag cggttacgat gcggtggata tcgatctggc acgcatcagt
    18721 aaagaaatca tcgacttcta tatcaaccag ggcgttaatc gtattggttt tattggcggt
    18781 gaagatgagc ctggcaaggc ggatattcgt gaggtcgcct ttgcggaata tggccgactg
    18841 aaacaagtgg tacgcgaaga ggatatctgg cgcggcggtt tttccagttc gtcgggttat
    18901 gaactggcaa aacaaatgct ggcgcgagaa gactatccga aggcactgtt tgttgcttcc
    18961 gattccattg ctatcggcgt actgcgggca attcatgaac gaggcctgaa catcccacag
    19021 gatatttcgc ttatcagcgt taacgatatc cccaccgcgc gatttacctt tccaccgctc
    19081 tccaccgtgc gcatccattc cgagatgatg ggaagtcagg gcgttaacct ggtgtatgaa
    19141 aaagcccgcg atggtcgcgc gctgccgctg ttagtcttcg ttcccagcaa attaaaactg
    19201 cgcggcacga cccgttaaat ccccttacac actgtccggc aatcgttttt gccggacagt
    19261 gctgccgttt attttcgtga tccagttaaa gtaaatgcat ttacctgcta ctttttagta
    19321 aaaattttac taaactcccc agcaattaca caaactacca tcaccatgaa tggttccgat
    19381 ttctctctac cgggaggccc tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac
    19441 cgacttgcgc cgcgtgcgta ctttttttca tatgattctg ttgcgcaagc gcgtaccttt
    19501 gcccgcgaaa ccagcagcct gtttctgccc ttaagcggtc agtggaattt ccactttttt
    19561 gaccatccgc tgcaagtacc agaagccttc acctctgagt taatggctga ctgggggcat
    19621 attaccgtcc ccgccatgtg gcaaatggaa ggtcacggca aactgcaata taccgacgaa
    19681 ggttttccgt tccccatcga tgtgccgttt gtccccagcg ataacccaac cggtgcctat
    19741 caacgtattt tcaccctcag cgacggctgg cagggtaaac agacgctgat taaatttgac
    19801 ggcgtcgaaa cctattttga agtctatgtt aacggtcagt atgtgggttt cagcaagggc
    19861 agtcgcctga ccgcagagtt tgacatcagc gcgatggtta aaaccggcga caacctgttg
    19921 tgtgtgcgcg tgatgcagtg ggcggactct acctacgtgg aagaccagga tatgtggtgg
    19981 tcagcgggga tcttccgcga tgtttatctg gtcggaaaac acctaacgca tattaacgat
    20041 ttcactgtgc gtaccgactt tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg
    20101 gtgctggaaa atctcgccgc ctcccctgtc gtcacgacgc tggaatatac cctgtttgat
    20161 ggcgaacgcg tggtgcacag cagcgccatt gatcatttgg caattgaaaa actgaccagc
    20221 gccagctttg cttttactgt cgaacagccg cagcaatggt cagcagaatc cccttatctt
    20281 taccatctgg tcatgacgct gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa
    20341 cgcgttggct tccgtgatat caaagtgcgc gacggtctgt tctggatcaa taaccgttat
    20401 gtgatgctgc acggcgtcaa ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga
    20461 atggatcgcg tcgagaaaga tctccagttg atgaagcagc acaatatcaa ctccgtgcgt
    20521 accgctcact acccgaacga tccgcgtttt tacgaactgt gtgatatcta cggcttgttt
    20581 gtgatggcgg aaaccgacgt cgaatcgcac ggctttgcta atgtcggcga tatcagccgt
    20641 attaccgacg atccgcagtg ggaaaaagtc tacgtcgagc gcattgttcg ccatattcac
    20701 gcgcagaaaa accatccgtc gatcatcatc tggtcgctgg gcaatgaatc cggctatggc
    20761 tgtaacatcc gcgcgatgta ccacgcagcg aaggcgctgg atgacacgcg actggtgcat
    20821 tacgaagaag atcgcgatgc tgaagtggtc gatattattt ccaccatgta cacccgcgtg
    20881 ccgctgatga atgagtttgg tgaatacccg catccgaagc cgcgcatcat ctgtgaatat
    20941 gctcatgcga tggggaacgg accaggcggg ctgacggagt accagaacgt cttctataag
    21001 cacgattgca ttcagggaca ttatgtctgg gaatggtgcg accacgggat ccaggcgcag
    21061 gatgacaacg gcaacgtctg gtataaattc ggcggcgact acggcgacta tcccaacaac
    21121 tataacttct gtcttgatgg tttgatctat tccgatcaga cgccgggacc gggcctgaaa
    21181 gagtacaaac aggttatcgc gccggtaaaa atccacgcgc tggatctgac tcacggcgag
    21241 ctgaaagtcg aaaataaact gtggtttacc acgcttgatg actacaccct gcacgcagag
    21301 gtgcgcgtcg aaggtgaaac gctcgcgacg cagcagatta aactgcgcga cgttgcgccg
    21361 aacagcgaag cccccttgca gatcacgctg ccgcagctgg acgcccgcga agcgttcctc
    21421 aacattacgg tgaccaaaga ttcccgcacc cgctacagcg aagccgggca ttctatcgcc
    21481 acttatcagt tcccgctgaa ggaaaacacc gcgcaaccag tgcctttcgc accaaataat
    21541 gcgcgtccgc tgacgctgga agacgatcgt ttgagctgca ccgttcgcgg ctacaacttc
    21601 gcgatcacct tctcaaaaat gagtggcaaa ccgacatcct ggcaggtgaa tggcgagtcg
    21661 ctgctgaccc gcgagccaaa gatcaacttc ttcaagccaa tgatcgacaa ccacaagcag
    21721 gagtacgaag ggctgtggca accgaatcat ttgcagatca tgcaggaaca tctgcgcgac
    21781 tttgccgtag aacagagcga tggtgaagtg ttgatcatca gccgcacagt tattgccccg
    21841 ccggtgtttg acttcgggat gcgctgcacc tacatctggc gcatcgctgc cgatggccag
    21901 gttaacgtgg cgctttccgg cgagcgttac ggcgactatc cgcacatcat tccgtgcatc
    21961 ggtttcacca tggggattaa cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg
    22021 ggcgaaaact acgccgacag ccagcaggct aacatcatcg atatctggcg cagcaccgtc
    22081 gatgccatgt tcgagaacta tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc
    22141 tggacggcac tgactaaccg ccacggcaac ggtctgctgg tggttccgca gcgcccaatt
    22201 aacttcagcg cctggcgcta tacccaggaa aacatccacg ctgcccagca ctgtaacgag
    22261 ctgcagcgca gtgatgacat caccctgaac ctcgaccacc agctgcttgg cctcggctcc
    22321 aactcctggg gcagcgaggt gctggactcc tggcgcgtct ggttccgtga cttcagctac
    22381 ggctttacgt tgctgccggt ttctggcgga gaagctaccg cgcaaagcct ggcgtcgtat
    22441 gagttcggcg cagggttctt ttccacgaat ttgcacagcg agaataagca atgaggatca
    22501 tcgataactt agaacagttc cgccagattt acgcctctgg caagaagtgg caacgctgcg
    22561 ttgaagcgat tgaaaatatc gacaacattc agcctggcgt cgcccactcc atcggtgact
    22621 cattgactta ccgcgtggag acagactccg cgaccgatgc gctatttacc gggcatcgac
    22681 gctattttga agtgcattac tacctgcaag ggcagcaaaa aattgaatat gcgccgaaag
    22741 agacattaca ggtagtggaa tattatcgtg atgaaactga ccgtgaatat ttaaaaggct
    22801 gcggagaaac cgttgaggtc cacgaagggc aaatcgttat ttgcgatatc catgaagcgt
    22861 atcggtttat ctgcaataac gcggtcaaaa aagtggttct caaagtcacc atcgaagatg
    22921 gttatttcca taacaaataa caactacggc ggcaaaagga gtttgccgcc accgctaccc
    22981 tacacatttt cggagatgtg ttatgtctga taccaaacgt aatacaatcg gcaaattcgg
    23041 cttgctctcg ctgacttttg ccgccgttta cagctttaac aacgttatca ataataatat
    23101 tgagcttgga ctggcctcgg caccgatgtt tttcctcgcg acgatttttt attttattcc
    23161 cttctgtctg atcatcgcag aatttgtttc gttaaataaa aactcagaag ccggtgtcta
    23221 cgcgtgggta aaaagttcgc tgggcggacg ttgggcattt attactgcct atacctactg
    23281 gttcgtaaac ctgttctttt tcacctcact gttgccgcgc gttattgctt atgcttcgta
    23341 tgccttcctc ggatacgaat atattatgac gccggttgcc accaccatta tcagtatggt
    23401 gctgttcgcc ttctccacct gggtttccac caacggggcg aaaatgttgg ggccaattac
    23461 ctccgtcact tcaacgctga tgctgctgtt aacgctctcc tacattttac tggcaggtac
    23521 ggcgctggtt ggcggcgtac agcctgctga ccccatcacc gttgacgcga tgatcccgaa
    23581 cttcaactgg gcgttcctcg gcgttaccac ctggatcttt atggccgcag gtggcgcgga
    23641 gtccgtcgct gtgtacgtta acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat
    23701 catcctcgcc gggattttta tcggcgtact gtattccgtc tcctcggtgc tgattaacgt
    23761 cttcgtcagc agcaaagagt tgaaatttac cggcggatcg gtgcaggtat tccacggcat
    23821 ggcggcgtat tttggtctac cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt
    23881 taccgcgatg ttcggttccc tgctgatgtg gaccgcaacg ccggtgaaaa ttttcttctc
    23941 cgaaatcccg gaaggcatct ttggtaagaa aaccgtcgaa ctgaacgaaa acggcgttcc
    24001 ggcgcgcgca gcgtggatcc agttcctgat cgtcatcccg ctgatgatta tcccgatgct
    24061 cggttccaat accgtgcagg atctgatgaa tactattatt aatatgaccg ccgcagcgtc
    24121 catgcttccg ccgttattca tcatgctggc ttacctgaat ttacgcgcca aattagatca
    24181 cctgccacgc gatttccgta tgggctcccg ccgcaccggt attatcgttg tttcaatgct
    24241 gattgcgata tttgccgtag ggtttgtcgc ttcgacattc ccgactggcg cgaatattct
    24301 gaccatcatt ttttataacg tcggcggtat tgttatcttc cttggctttg cgtggtggaa
    24361 atacagtaaa tatataaagg gattaacggc tgaagagcgc catattgaag cgacgccagc
    24421 cagcaatgtt gattaagcag aataataaaa aatgagcagg cattttccct cttcctggat
    24481 gaggagaggg aaaatagttt ctgcctttat atttttataa ttacaacgat aaaaggctgt
    24541 actttttctt tagctcatgg attaacacaa tgaaattaat cactgcacca tgcagagcat
    24601 tacttgctct gccgttttgc tacgcctttt ctgcggcagg agaagaagca cgtccggcag
    24661 aacatgacga cacaaaaaca cctgcaatta cctcgacatc ttctccttca tttcgttttt
    24721 acggcgaatt aggggttggt ggatatatgg atttagaggg tgagaataaa cataaataca
    24781 gcgacggtac ctatattgaa ggtggcctgg agatgaagta cggctcctgg ttcggcctga
    24841 tttacggcga aggctggacc gtgcaggccg accacgacgg caatgcctgg gtgccagacc
    24901 atagctgggg tggtttcgag ggcggaatta accgtttcta tggcggttat cgtaccaatg
    24961 atggcaccga aatcatgctc agtctgcgtc aggattcctc gctggatgac ctgcaatggt
    25021 ggggcgactt cacccccgat ctgggctacg tcattcccaa tacccgcgac attatgaccg
    25081 cgctgaaggt gcagaactta agcggcaact ttcgttatag cgtcaccgcc acacctgccg
    25141 gacatcatga cgaaagcaaa gcctggctac attttggcaa gtacgatcgc tatgacgaca
    25201 aatacaccta tccggcaatg atgaacggtt acatccagta tgaccttgcc gaaggcatca
    25261 cctggatgaa cggtctggaa atcaccgacg gcacaggaca gctctatctc acgggcctgc
    25321 taactcctaa ctttgccgct cgcgcctggc accataccgg acgcgccgac gggctggacg
    25381 taccgggaag tgaaagtggg atgatggtga gcgccatgta tgaagcgtta aagggcgttt
    25441 atctctccac cgcttacacc tacgccaaac atcgccctga ccacgctgac gatgaaacca
    25501 cctctttcat gcagtttggt atctggtacg aatacggcgg cggacgtttc gccacggctt
    25561 ttgatagccg cttctacatg aaaaatgcct ctcacgatcc cagcgaccaa atcttcctga
    25621 tgcaatattt ctactggtaa taaggactgt aatttcatga aaataaaaac tattttaacg
    25681 ccagtaacct gcgctctgct gataagtttt tccgcccatg ccgctaacgc cgacaattat
    25741 aaaaacgtga ttaaccgtac tggcgcgccg cagtacatga aggattacga ttacgacgat
    25801 caccagcgtt ttaatccgtt tttcgatctc ggagcctggc atggtcatct gttgccagac
    25861 ggccctaaca ccatgggcgg ctttccgggc gttgcgctgc tgacggaaga gtacatcaac
    25921 tttatggcca gcaatttcga ccgcctgacc gtctggcagg acggcaagaa agtcgacttt
    25981 acgctggagg catacagtat tcccggtgcg ctggtgcaaa aactgacagc aaaagatgtg
    26041 caagtcgaaa tgactctgcg cttcgccacg ccgcgcacgt cactactgga aaccaaaatc
    26101 accagcaata aaccgctgga tctggtgtgg gacggcgaac tgctggaaaa actggaagcg
    26161 aaagaaggga aaccgctttc cgataaaacc attgctggcg aataccctga ctatcagcgc
    26221 aaaatcagcg ccacccgtga tggcctgaaa gtcacctttg gcaaagtgcg cgccacctgg
    26281 gatctgctga cctccggcga atcggaatat caggtgcata aatccctgcc ggtgcagact
    26341 gaaatcaacg gtaatcgctt taccagtaag gcgcatatca acggttcgac cacgctctac
    26401 accacctatt cccatctgct gaccgctcag gaagttagca aagagcaaat gcagatccgc
    26461 gatattctgg cgcgtccggc gttttatctc accgcctcgc agcaacgctg ggaagaatac
    26521 ctgaagaaag ggttaaccaa tccggatgcg acgccggaac agacgcgcgt cgcggtgaaa
    26581 gcgatcgaaa cgctcaacgg taactggcgc tcaccgggcg gtgcggtgaa atttaacacc
    26641 gttacaccgt cggtgaccgg gcgctggttc tccggcaatc agacctggcc gtgggatacc
    26701 tggaagcagg cgtttgcgat ggcgcatttc aatccggaca tcgccaaaga gaatatccgc
    26761 gcggtcttct cctggcagat ccagcctggc gatagcgtgc gtccgcagga tgtgggcttt
    26821 gtccccgacc tgatagcgtg gaaccttagc cccgagcgtg gtggcgatgg cggcaactgg
    26881 aacgaacgta ataccaaacc cagccttgcc gcctggtcgg tgatggaagt gtacaacgtc
    26941 acccaggata aagcctggtt ggcagagatg tacccgaaac tggtggccta tcacgactgg
    27001 tggttacgta accgcgatca caacggcaac ggcgtgccgg aatatggcgc gacccgcgac
    27061 aaagcccaca acactgagag cggcgagatg ctgtttacgg tgaagaaagg caacaaagaa
    27121 gagacgcagt ctgggctgaa caactacgcc cgcgtggtgg agaaaggcca gtacgacagt
    27181 ctggaaattc cggcacaggt cgctgcgtcg tgggaatcgg ggcgtgatga tgccgccgtc
    27241 tttggtttta tcgacaaaga acagttggat aaatatgtcg ctaatggcgg caaacgtagc
    27301 gactggacgg tgaaattcgc cgaaaaccgc agtcaggacg gaacgttgct gggctactcg
    27361 ctattgcagg agtcggtgga tcaggccagc tatatgtaca gcgataacca ttatctggcg
    27421 gagatggcaa ccatcctcgg taagccagaa gaggccaaac gctatcgcca gttggcacag
    27481 cagctcgcgg actacatcaa cacctgtatg ttcgacccga ctacccagtt ctactatgac
    27541 gtacgtattg aagataaacc actggcgaac ggctgcgcgg gcaaaccgat tgttgagcgc
    27601 ggtaaagggc cggaaggctg gtcgccgctg tttaacggtg cggcaacgca ggccaatgcc
    27661 gacgcggtgg tgaaggtgat gctcgatcct aaagagttca acacctttgt cccgctggga
    27721 acggcggcgt taaccaatcc ggcttttggc gctgatatct actggcgtgg gcgcgtatgg
    27781 gtggatcagt tctggtttgg tctgaaaggg atggagcgtt acggttatcg cgatgatgcc
    27841 ctgaaactgg cggatacgtt cttccgtcac gccaaaggat taaccgccga tggcccaatt
    27901 caggagaatt acaacccgct gactggcgca cagcaaggcg caccaaattt ctcctggagt
    27961 gccgcgcatt tgtatatgtt gtataacgat tttttccgta agcaataatt tgcaactgct
    28021 ggcggatgcg gcgtaaacgc cttatccgcc ctacatgtgc aatcctcctg gccggataaa
    28081 acgcgacaag cgtcgcatcc ggcatctgca ccgtgccact ggcggatgcg gcgtgaacgc
    28141 cttatccgcc ctacatgtgc aatcccccag gccggataaa acgcggcaag cgtcgcatcc
    28201 ggcgttacca ccgggcgtat tctttttgaa tcccatcaca aaccccgcac tccccttttc
    28261 ccttttctcc ggcgacggct aaattagaac tcatccgacc acataacaat tattttacat
    28321 actggacaat tttatgagct acccgtcgct gttcgccccg ctggatttag gttttaccac
    28381 gttaaaaaac cgcgtgttga tgggctcaat gcacaccggg ctggaggaat acccggacgg
    28441 tgccgagcgg ctggcagcgt tttatgccga acgcgcccgt cacggcgtgg cgctgattgt
    28501 cagcggcggc attgcaccag atttaacagg cgttggcatg gaaggtggcg caatgctcaa
    28561 cgacgccagc cagatcccac accatcgcac cattaccgaa gcggtacatc aggaaggcgg
    28621 caaaatagcc ctgcaaattt tgcataccgg gcgctacagc taccaaccgc atctagtcgc
    28681 tccctccgca ttgcaggccc ccatcaaccg tttcgttcct cacgaactca cccatgaaga
    28741 gatcctgcaa ctgatcgacg atttcgccca ctgcgcgcaa ctggcacggg aggcaggata
    28801 cgacggtgta gaggtaatgg ggtccgaagg gtatttgatc aacgaatttc tgacgctgcg
    28861 caccaatcag cgtagtgacc agtggggcgg cgattaccgc aaccggatgc gatttgccgt
    28921 agaagtagtg cgtgcggtgc gcgaacgcgt cggcaacgac ttcattatta tctaccgact
    28981 gtcgatgctc gacctggtcg aagacggcgg gacttttgcc gaaacggtag agctggcgca
    29041 ggccattgaa gcggcgggcg cgaccattat caacaccggc attggctggc atgaagcacg
    29101 tattccgacc attgccacgc ccgtgccgcg cggcgcattt acttgggtca cgcgcaaact
    29161 gaaaggccac gtttcgctgc cgctggtgac caccaaccgg attaacgatc cgcaggttgc
    29221 cgacgacatt ctctcgcgcg gcgatgccga tatggtgtcg atggcacgac cgtttcttgc
    29281 tgatgcggaa ctgctgtcaa aagcgcaatc ggggcgagcc gatgagatca acacctgcat
    29341 tggctgcaat caggcctgcc tcgatcagat cttcgttggc aaagtcacct cgtgcctggt
    29401 gaatcctcgc gcctgccacg aaaccaaaat gccaatcctt cctgccgtgc agaaaaaaaa
    29461 tctggcggtg gtcggtgcgg gacctgctgg gctggcgttt gccattaacg cggcggcgcg
    29521 tgggcatcag gtaacattgt ttgatgccca cagcgagatc ggcgggcagt ttaatatcgc
    29581 caaacagatc cccggcaaag aggagtttta cgaaacgctg cgctattacc accggatgat
    29641 cgaagtgacg ggcgtgacgc tgaaactcaa tcacaccgtg acggcggatc agttacaggc
    29701 gttcgatgaa acgatcctcg ccagtgggat cgtgccgcgc gttccgccta tcgacgggat
    29761 cgatcatccg aaggtattga gttatctcga tgtactgcgc gacaaagcgc cggtaggcaa
    29821 caaggttgcc atcatcggtt gtggcgggat tggttttgat acggcgatgt atttaagtca
    29881 gccgggcgaa tccaccagcc agaatatcgc cgggttctgt aatgagtggg ggatcgacag
    29941 cagcctacaa caggctggtg gcttaagccc gcagggaatg cagatcccgc gtagcccacg
    30001 gcaaattgtg atgctccagc gcaaagccag caaaccggga caggggttag gcaaaaccac
    30061 cggctggatc catcgcacta ccctgctctc gcgcggtgtg aaaatgatcc caggcgtcag
    30121 ttaccagaag atcgacgatg acgggctgca tgtggtgatc aacggcgaaa cacaggtatt
    30181 cgcggtggac aatgtggtga tctgcgcagg gcaagagcca aaccgcgcgc tggcgcaacc
    30241 gctgattgat agcgggaaaa ctgtgcattt aattggcggc tgcgatgtgg ctatggagct
    30301 ggacgcacga cgggcaattg cccagggaac acggctggcg ctggaga
//
LOCUS       DAZFVO020000046        29963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFVO020000046 DAZFVO020000000
VERSION     DAZFVO020000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.046"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKNB_RS09600"
                     /old_locus_tag="pgaptmp_001920"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKNB_RS09600"
                     /old_locus_tag="pgaptmp_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HGU4195445.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKNB_RS09605"
                     /old_locus_tag="pgaptmp_001921"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKNB_RS09605"
                     /old_locus_tag="pgaptmp_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HGU4195446.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKNB_RS09610"
                     /old_locus_tag="pgaptmp_001922"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKNB_RS09610"
                     /old_locus_tag="pgaptmp_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HGU4195447.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPMYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKNB_RS09615"
                     /old_locus_tag="pgaptmp_001923"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKNB_RS09615"
                     /old_locus_tag="pgaptmp_001923"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HGU4195448.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKNB_RS09620"
                     /old_locus_tag="pgaptmp_001924"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKNB_RS09620"
                     /old_locus_tag="pgaptmp_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HGU4195449.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKNB_RS09625"
                     /old_locus_tag="pgaptmp_001925"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKNB_RS09625"
                     /old_locus_tag="pgaptmp_001925"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HGU4195450.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKNB_RS09630"
                     /old_locus_tag="pgaptmp_001926"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKNB_RS09630"
                     /old_locus_tag="pgaptmp_001926"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HGU4195451.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKNB_RS09635"
                     /old_locus_tag="pgaptmp_001927"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKNB_RS09635"
                     /old_locus_tag="pgaptmp_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HGU4195452.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKNB_RS09640"
                     /old_locus_tag="pgaptmp_001928"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKNB_RS09640"
                     /old_locus_tag="pgaptmp_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HGU4195453.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKNB_RS09645"
                     /old_locus_tag="pgaptmp_001929"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKNB_RS09645"
                     /old_locus_tag="pgaptmp_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HGU4195454.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKNB_RS09650"
                     /old_locus_tag="pgaptmp_001930"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKNB_RS09650"
                     /old_locus_tag="pgaptmp_001930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HGU4195455.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKNB_RS09655"
                     /old_locus_tag="pgaptmp_001931"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKNB_RS09655"
                     /old_locus_tag="pgaptmp_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HGU4195456.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKNB_RS09660"
                     /old_locus_tag="pgaptmp_001932"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKNB_RS09660"
                     /old_locus_tag="pgaptmp_001932"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4195457.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(10883..11308)
                     /gene="exbD"
                     /locus_tag="ACHKNB_RS09665"
                     /old_locus_tag="pgaptmp_001933"
     CDS             complement(10883..11308)
                     /gene="exbD"
                     /locus_tag="ACHKNB_RS09665"
                     /old_locus_tag="pgaptmp_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HGU4195458.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11315..12049)
                     /gene="exbB"
                     /locus_tag="ACHKNB_RS09670"
                     /old_locus_tag="pgaptmp_001934"
     CDS             complement(11315..12049)
                     /gene="exbB"
                     /locus_tag="ACHKNB_RS09670"
                     /old_locus_tag="pgaptmp_001934"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HGU4195459.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12301..13488
                     /gene="metC"
                     /locus_tag="ACHKNB_RS09675"
                     /old_locus_tag="pgaptmp_001935"
     CDS             12301..13488
                     /gene="metC"
                     /locus_tag="ACHKNB_RS09675"
                     /old_locus_tag="pgaptmp_001935"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGU4195460.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13628..14287
                     /gene="yghB"
                     /locus_tag="ACHKNB_RS09680"
                     /old_locus_tag="pgaptmp_001936"
     CDS             13628..14287
                     /gene="yghB"
                     /locus_tag="ACHKNB_RS09680"
                     /old_locus_tag="pgaptmp_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HGU4195461.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14327..15226)
                     /gene="yqhC"
                     /locus_tag="ACHKNB_RS09685"
                     /old_locus_tag="pgaptmp_001937"
     CDS             complement(14327..15226)
                     /gene="yqhC"
                     /locus_tag="ACHKNB_RS09685"
                     /old_locus_tag="pgaptmp_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HGU4195462.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15420..16583
                     /gene="yqhD"
                     /locus_tag="ACHKNB_RS09690"
                     /old_locus_tag="pgaptmp_001938"
     CDS             15420..16583
                     /gene="yqhD"
                     /locus_tag="ACHKNB_RS09690"
                     /old_locus_tag="pgaptmp_001938"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HGU4195463.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16688..17515
                     /gene="dkgA"
                     /locus_tag="ACHKNB_RS09695"
                     /old_locus_tag="pgaptmp_001939"
     CDS             16688..17515
                     /gene="dkgA"
                     /locus_tag="ACHKNB_RS09695"
                     /old_locus_tag="pgaptmp_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HGU4195464.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17715..18641
                     /locus_tag="ACHKNB_RS09700"
                     /old_locus_tag="pgaptmp_001940"
     CDS             17715..18641
                     /locus_tag="ACHKNB_RS09700"
                     /old_locus_tag="pgaptmp_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HGU4195465.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18692..18949
                     /gene="yqhH"
                     /locus_tag="ACHKNB_RS09705"
                     /old_locus_tag="pgaptmp_001941"
     CDS             18692..18949
                     /gene="yqhH"
                     /locus_tag="ACHKNB_RS09705"
                     /old_locus_tag="pgaptmp_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HGU4195466.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(18992..21211)
                     /locus_tag="ACHKNB_RS09710"
                     /old_locus_tag="pgaptmp_001942"
     CDS             complement(18992..21211)
                     /locus_tag="ACHKNB_RS09710"
                     /old_locus_tag="pgaptmp_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HGU4195467.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21322..22734)
                     /gene="ftsP"
                     /locus_tag="ACHKNB_RS09715"
                     /old_locus_tag="pgaptmp_001943"
     CDS             complement(21322..22734)
                     /gene="ftsP"
                     /locus_tag="ACHKNB_RS09715"
                     /old_locus_tag="pgaptmp_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HGU4195468.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22809..23546)
                     /gene="plsC"
                     /locus_tag="ACHKNB_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /gene_synonym="parF"
     CDS             complement(22809..23546)
                     /gene="plsC"
                     /locus_tag="ACHKNB_RS09720"
                     /old_locus_tag="pgaptmp_001944"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HGU4195469.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(23779..26037)
                     /gene="parC"
                     /locus_tag="ACHKNB_RS09725"
                     /old_locus_tag="pgaptmp_001945"
     CDS             complement(23779..26037)
                     /gene="parC"
                     /locus_tag="ACHKNB_RS09725"
                     /old_locus_tag="pgaptmp_001945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGU4195470.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSCDSEE"
     gene            complement(26583..27065)
                     /locus_tag="ACHKNB_RS09730"
                     /old_locus_tag="pgaptmp_001946"
     CDS             complement(26583..27065)
                     /locus_tag="ACHKNB_RS09730"
                     /old_locus_tag="pgaptmp_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HGU4195471.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27118..27510)
                     /gene="ygiW"
                     /locus_tag="ACHKNB_RS09735"
                     /old_locus_tag="pgaptmp_001947"
     CDS             complement(27118..27510)
                     /gene="ygiW"
                     /locus_tag="ACHKNB_RS09735"
                     /old_locus_tag="pgaptmp_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HGU4195472.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27662..28321
                     /gene="qseB"
                     /locus_tag="ACHKNB_RS09740"
                     /old_locus_tag="pgaptmp_001948"
     CDS             27662..28321
                     /gene="qseB"
                     /locus_tag="ACHKNB_RS09740"
                     /old_locus_tag="pgaptmp_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HGU4195473.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28318..29667
                     /gene="qseC"
                     /locus_tag="ACHKNB_RS09745"
                     /old_locus_tag="pgaptmp_001949"
     CDS             28318..29667
                     /gene="qseC"
                     /locus_tag="ACHKNB_RS09745"
                     /old_locus_tag="pgaptmp_001949"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HGU4195474.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29713..>29963)
                     /locus_tag="ACHKNB_RS09750"
                     /old_locus_tag="pgaptmp_001950"
     CDS             complement(29713..>29963)
                     /locus_tag="ACHKNB_RS09750"
                     /old_locus_tag="pgaptmp_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HGU4195475.1"
                     /translation="FNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTPLIILI
                     LRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7365 a         8025 c         7593 g         6980 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacatcgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac gaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acaggctctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagt gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacagcgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcaacgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 tcagacgctg tagcgacacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gagccatcac gccgatgtag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc agtgccgatc agcaggtcaa acgcgatcca cacgccccac ggaaagccgc
     4321 cgttcaggtc ggagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttttggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aaattgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 acccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tttgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcacgggtga ccaatcgcca
     5701 ccggccacac accgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcgcg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cggtttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatggct gctgcgcctt
     6121 ccgccgcttt gcggatgtga tccacaatag gctcaccggc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacca ctaacacata ctgccctttg tacttctcga
     6241 gagcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agtacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatattgtca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tcccgtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca ttacccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc accaccacgg
     7741 ctggcgtttt gccgctcatc cttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ctaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt cgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcagtacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cgtacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgcatc gtgaagggaa
     8941 taaagttcgt ggtctgacgc cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gctgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagaacgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattcctga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct attctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat ttttatttcg ctttggcggt ttcttcgccg accagaccta
    10921 tcttcaggta acccgcctga tgcagcgtat ccattacctt catcaacgtc tcgtaatcga
    10981 cggttttatc cgctcggaag aagatggtgg tgtctttctt gccttcggtt aacgcattca
    11041 acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc gagttgtctg
    11101 ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg gtagaagcag
    11161 gcaaattcac cttcacatct accgtcgcta acggtgccgc caccataaag ataatcagca
    11221 gaaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg ccgttatcat
    11281 cgaggttttc gttaagatgc attgccatcg gacattatcc tgcgcgtaat ttttgtgcga
    11341 cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt tgcagcaaca
    11401 atacctgcgc tgcaacatca cccagcatcg ctttaaagcc gccaatctgg cgtgcaaata
    11461 cgttatagat aacgaccgca ggaatcgctg ccacgaggcc gattgccgtt gctaacagag
    11521 cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc gcgataccaa
    11581 taaagctgtt catgatgccc cagaccgtac caaacagacc aacgaacgga gaaatcgcgc
    11641 cgatggttgc cagatagccg ttaccgcggc ccatttgacg acccactgcg gcgacccgac
    11701 gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg ccttctgaca
    11761 gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg ctaccaaaat
    11821 cagcggcgat atcgttggcc tggtttaagg aacgcgcttc agccagcagt tgctgctcgc
    11881 gcttaaggcg acgcttctga ttgaagaact ctacgctctt actgaagaag attgcccagg
    11941 tgactacgga ggccaaaata agcccaatca tcacgcactt aacgacaata tcggcgtgct
    12001 gatacatacc ccagacggaa aggtccgtct gcattaaatt attacccact gtgtatctcc
    12061 aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa cgacgtcgaa ttgatagtcg
    12121 ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg tttacgcagt
    12181 aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat tcttcaggat
    12241 gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag gaatcccgac
    12301 atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact
    12361 ctcggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtctttga cagtgtggaa
    12421 gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg acggcgcgga
    12481 acgttaaccc atttctcctt acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgc
    12541 gtgctatttc cctgcggggc ggcagcggtt gctaattcca ttcttgcttt tgtcgaacag
    12601 ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa
    12661 atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg tgccgatatc
    12721 gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccagg ctccatcacc
    12781 atggaagtcc acgacgttcc ggcgattgtt gccgccgtgc gcagtgtggt cccggatgcc
    12841 atcattatga tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc
    12901 atcgatgttt ccattcaggc cgctaccaaa tatctggttg ggcattcaga tgcgatgatt
    12961 ggcactgccg tgtgcaatgc ccgttgctgg gagcagctgc gggagaatgc ctatctgatg
    13021 ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg cacattaggt
    13081 gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ctgaatggct ggcagaacat
    13141 ccgcaagttg cgcgagttaa ccaccctgct ctgcctggca gtaaaggcca cgaattctgg
    13201 aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcagt
    13261 aatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc ctactcgtgg
    13321 ggcgggtatg aatcgttgat cctggcgaat caaccagaac atatcgccgc cattcgccca
    13381 caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct ggaagatgtc
    13441 gacgatctga ttgccgatct ggacgccggt tttgcgcgaa ttgtataaca ttgccacttt
    13501 tggacaattt tgcagacatt taattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag
    13561 gcgtcccgga cgaatcagga gtacaatagg caaataaagg cttaaacgct gttccacagg
    13621 aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg actttgccgc
    13681 gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca cgctgttttt
    13741 agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga tactggcagg
    13801 cgcattgatt gctcaggggg ttatggattt tctgcctacg attgcgattc tgaccgccgc
    13861 agcaagtctg ggctgctggc taagttatat tcaggggcgc tggttaggga ataccaaaac
    13921 ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca cctgcatgtt
    13981 tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg tccgtacgct
    14041 gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgcttccagt ttttcaactg
    14101 gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt ggctatgcct taagtatgat
    14161 tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc tgccaattgc
    14221 cttgttaacc gctggcttgt taggcacgct gtttgtggtg attaaaaaaa aatactgtaa
    14281 cgcctgacga ttttccccgt tcccggctgc tgtaccggga acgtatttaa ttcccctgca
    14341 tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac
    14401 ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc tgctgcgcta gccttcatgc
    14461 cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa tactgcaacg
    14521 gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc cgatacgctc atgttggctt
    14581 ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatc cgtttcagca
    14641 cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac gcgccgccgc
    14701 aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg agaatacgcg
    14761 catccagtgg tcgctccatc acgtcgagta accgctccgc cgcgcataaa atctcttctg
    14821 ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc tcatcttcgc
    14881 caatgtccat caacagttcc tgtaactgca aaatatcgac attgagacgc aaccctgcca
    14941 gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc gtcagcagca
    15001 ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt ttatgcccgg
    15061 aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa ggcgtctcgc
    15121 catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta tttttcagtt
    15181 tattaacttt atccgccagc aagcggcaaa tctcttcacg tttcatatcg cgtaatttct
    15241 taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca gcactctgga
    15301 gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg cgatcacaat
    15361 gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg gagcaagtaa
    15421 tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc gcaatcgctg
    15481 gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggc ggcggcagcg
    15541 tgaaaaaaac cggcgttctc gatcaagttc tggatgccct gaaaggcatg gacgtgctgg
    15601 aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc gtgaaactgg
    15661 ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg cggttctgta ctggacggca
    15721 ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg cacattctgc
    15781 aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag
    15841 caaccggttc agaatccaac gcaggcgcgg tgatctcccg taaaaccaca ggcgacaagc
    15901 aggcgttcca ttctgcccat gttcagccgg tatttgccgt gctcgatccg gtttatacct
    15961 acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg
    16021 aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca gaaggcattt
    16081 tgctgacgct aatcgaagat ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc
    16141 gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg tttgattggc gctggcgtac
    16201 cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac ggtctggatc
    16261 acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc gagaccaagc
    16321 gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt tccgacgatg
    16381 agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta ggcgtgccga
    16441 cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg aaaaaactgg
    16501 aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgctggat gtcagccgcc
    16561 gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt cgggcatttc
    16621 gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc atataaagga
    16681 ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca tgccccagtt
    16741 gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc aaaaagcgtt
    16801 agaagtgggt tatcgctcga ttgataccgc cgcggcctac aagaacgaag aaggtgtcgg
    16861 caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca ctaagctgtg
    16921 gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa aactccagct
    16981 tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc attatgtcga
    17041 agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca tcggcgtgtg
    17101 caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga cgcctgtgat
    17161 aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct ggaacgcgac
    17221 acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag gcgttttcga
    17281 tcagaaagtc attcgcgatc tggcagataa atacggcaaa accccggcgc agattgttat
    17341 ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac cttcacgtat
    17401 tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg gcgaaattgc
    17461 aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg gctaacatgc
    17521 aaattctccc ggtggcggta atgttccgct accggacttt tcagaaatca tttattcccc
    17581 tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa ggacatcatc
    17641 atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa acgcaacgga
    17701 gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca agttttaccg
    17761 tacacgcaca gcccccctca ctgaccgtag aacaaacagt ccggcatatt tatcagaact
    17821 ataaatcaga tgccactgcc ccttattttg gtgaaaccgg agagcgggcg ataacttctg
    17881 cgcgtattca acaggcgctt accctgaacg acaatcttac gctgccgggc aatattggct
    17941 ggctggatta tgatccggtt tgtgattgtc aggattttgg cgatctggtg ctagaaagcg
    18001 ttgcgataac ccaaactgac gccgatcatg ccgatgccgt tgtgcgcttt cgtatcttta
    18061 aagatgataa agaaaagacc acgcagacac tgaaaatggt ggcggaaaat ggtcgttggg
    18121 tcattgacga tattgtcagc aatcatggca gcgtcttaca agcagttaat agcgagaatg
    18181 aaaaaacgct ggccgcttta gcttcgttgc aaaaagaaca gccggaagcc tttgttgccg
    18241 aactctttga acatattgct gattatagct ggccgtggac gtgggtggtt tccgactctt
    18301 accgccaggc ggttaatgcc ttctataaaa ccaccttcaa gacggccaat aatcccgatg
    18361 aagatatgca aatagaacgg caatttattt acgacaatcc gatctgtttt ggcgaagagt
    18421 cgctattttc acgcgttgat gaaattcgag tcctggagaa aaccgccgat tccgcccgca
    18481 ttcatgttcg ttttacgctg accaatggca acaacgaaga gcaagaactg gttttacagc
    18541 ggcgcgaagg caagtgggaa atcgctgatt ttatccgccc gaacagcggc agcctactta
    18601 agcagattga ggcaaaaacc gccgccagat taaagcaatg agctgaatta aataacaatt
    18661 agccggaaca ataaataaaa gggaacacta tatgaaaacg attttcaccg tgggagctgt
    18721 tgttctggca acctgcttgc tcagtggctg cgtcaatgag caaaaggtca atcagctggc
    18781 gagcaatgtg caaacattaa atgccaaaat cgcccggctt gagcaggata tgaaagcact
    18841 acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc gtcttgatgc
    18901 tcaggactat tttgattgcc tgcgctgctt gcgtatgtac gcagaatgat aaaaaaatcc
    18961 ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct ttagggcgtt
    19021 tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag
    19081 gcgtctgacg ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt
    19141 tctggcgacg agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac
    19201 ggctgccaat cagatgcttt ttgcccatcg cttccagcgc ctggcggatt aacggccagt
    19261 ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg tcgcccttcg
    19321 gtacgaagac gtcttcgctc ttataaccaa tcttcgccag cgggtttttc ccggtgtaat
    19381 acatggtggt tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga
    19441 aacgatgctt tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt
    19501 gcgcggagat gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat
    19561 cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg
    19621 gcccttcttc ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca
    19681 actctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct gaggcgatga
    19741 ggattttttt aatgcctttc agatcacgcg cacggcgata gaggttgatc gtcggttcgt
    19801 ggttagtgtc catgtgcgga caaatatccg gataaacgca cgacaaacgg cgacaagttt
    19861 gttcagcgcg tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat
    19921 cggaaatcac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga
    19981 tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg ctcggtaata gaacagaaag
    20041 aacagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg
    20101 gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc gcaaaaacgc
    20161 tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca tagcggtcac
    20221 cgtgtttttg catcaatgcg cgggcgcagc ctgggttggt ttcgtggtgc agaatgcgcg
    20281 aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt
    20341 aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct
    20401 ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga
    20461 ttgggtcgat ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca
    20521 gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc
    20581 ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca
    20641 tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat
    20701 gtgcggtacg gcgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg
    20761 cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt
    20821 tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca
    20881 tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcatga
    20941 aatcgtcttt gctgctccag tctggctggg cgatgatccc gacgcgaaag ccctgtgctt
    21001 ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag
    21061 taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca cgagacatcg
    21121 gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc
    21181 gatccggttg gatcagggag atagagctca tattgcttcc agaaatgata aaaaaataat
    21241 caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaggtattc catactcgcc
    21301 ctcctcgggc gagtatgaag attacggtac cggattgacc aaaagttgcc ctatcgagcc
    21361 acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg
    21421 ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc
    21481 tctgtcttcc gggaacggca tcgcgccatt cacgttacgg atctggaaca ttacgccttc
    21541 aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg
    21601 cgcggtgaca tcaatacggt tgacgtccca cagctgtcca ttaatacccg ggtcatcacc
    21661 cagactgata tcgcgactgc gaattggcga accagccatg atttcagttg gcagcaagcg
    21721 catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag
    21781 ggtagaaacc agaatactgg atggctcaaa gaagccgcga atacgatcaa caatgctcgc
    21841 cgcttcgcca caggtgatcg acacttcatc gccgttgctc atatccacca gaatctcgcg
    21901 gcgctcgccc ggtgccagcg aaagttgctt caccgataca ggagcaggca ggaatccctg
    21961 atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat agcgacgaga
    22021 gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct
    22081 ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta
    22141 ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac
    22201 accataatgg ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc
    22261 ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag
    22321 agtagctgcg ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat
    22381 ccgtgccgga ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac
    22441 attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat
    22501 agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa
    22561 tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag
    22621 cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc
    22681 tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc
    22741 tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga
    22801 ttgccgactt aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg
    22861 attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca
    22921 tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga
    22981 ttaagattaa tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc
    23041 gcaattgccg cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg
    23101 gttccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc
    23161 gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac
    23221 cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc
    23281 ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata
    23341 tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg
    23401 aacagcggcg caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc
    23461 gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta
    23521 ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga ctggcattgt
    23581 acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt
    23641 taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg
    23701 aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa
    23761 caggccctcc ccggcatatt actcttcgct atcacagctg ctggcacggc gaggagagtc
    23821 gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc
    23881 gccagtgact ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat
    23941 ggtcagcgtg ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc
    24001 ggcttctgcc gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag
    24061 atcgcttacc gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga
    24121 agcatcttca atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc
    24181 cttacctgca cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc
    24241 atcggaagcc atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc
    24301 cccaggcggc aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag
    24361 cgtaatcggg tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt
    24421 gctcttacct ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc
    24481 gtcgatatca tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac
    24541 aggttcagac ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa
    24601 cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag
    24661 gttattcatt ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct ctttttccag
    24721 ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag
    24781 ttcgaggatc gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt
    24841 cggttcatct tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag
    24901 caaaccttcc aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg
    24961 gcgcacggta tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac
    25021 cgccggacga ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc
    25081 ggtggtagcg aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac
    25141 aatcaccaga cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg
    25201 cagctttttg ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg
    25261 atgcggtaat gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg
    25321 cactgaacca cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc
    25381 cgcttcagtc ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt
    25441 cggctggtcg attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat
    25501 atcggtcgcc atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg
    25561 agcaggcagc attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt
    25621 cccctgcccc agctcgctca atagcagctc ggaatatttc gacaaccggg attcggtgta
    25681 acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac
    25741 cagcggataa cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcgct
    25801 atcgccgtgc ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa
    25861 tttggcgctg gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg
    25921 tttaagacca tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt
    25981 taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa
    26041 ttctgattcc tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca
    26101 ttatctgccg cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga
    26161 cggcgataaa ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca
    26221 tagagggcag cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg
    26281 cgattcgaga agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac
    26341 tgtaagtata cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc
    26401 cataagtgcg gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa
    26461 tttcgcgtat acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg
    26521 tggcattgtt cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc
    26581 catcacgcca acggcacata aacatccgtt tccagtcgct gctctgtcac cccttcggca
    26641 agattggtgt agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc
    26701 cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg
    26761 gccacggcat aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga
    26821 atcggttcgc taacgctgcc gcagatatca aagcgaaacg cttccggtgc ggtggtggca
    26881 gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct
    26941 ttacgccaca tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc
    27001 ggcaacatcg ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt
    27061 gtcattttgc ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta
    27121 cggatttact ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac
    27181 ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg
    27241 gtcgatatca acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat
    27301 gcgttcaacg atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct
    27361 ttctacggtc gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc
    27421 cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc ggtgcgctgc acagggccat
    27481 tactgcgatt actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg
    27541 agcatttaac aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttagtgctg
    27601 tacagagcgc gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa
    27661 aatgcgaatt ttactgatag aagatgacat gctgattggc gacggcatca aaacgggcct
    27721 tagtaaaatg ggttttagcg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct
    27781 ttatagcgcg ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg
    27841 cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag ccggtactga tcctgaccgc
    27901 gcgcgatgcg ctggaggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg
    27961 taaacctttt gcgttgatag aagtcgccgc caggctggaa gctctgatgc gccgaaccaa
    28021 cggccaggcc agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat
    28081 cgccacgctg gctggcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt
    28141 actgatgcgt aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac
    28201 ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa
    28261 actcggtagt gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg
    28321 aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc
    28381 tcggtgacct ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat
    28441 gaattgttcg acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac
    28501 gaaatcaacg cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt
    28561 gatgacgatg cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaacgat
    28621 ggcgataacg gagaagatat tccttatagc tatcaacggg aaggttttgc tgacgggcaa
    28681 ctggtgggcg ataaagatca atggcgtttt gtctggatga cctcacctga tggcaaatat
    28741 cgcatcgttg ttggtcagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc
    28801 gggcaattga tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta
    28861 ttgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg cgctgcgtat gcgtgaccct
    28921 gactcggaaa aaccactaaa cgcaactggc gtacccagcg aagtgcgtcc gttggttgag
    28981 tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg ttcgtgaacg acgctttacc
    29041 tccgacgcag ctcacgaact acgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt
    29101 gcgcagcttt ctgacgatga tccgcaagcg cggaaaaaag cgctgcttca attacattac
    29161 gggatcgatc gcgctactcg tctggttgat caactgctca ctctatcgcg gctggactca
    29221 ctggataacc ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg
    29281 atggatattt accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgcc
    29341 cacggcatca aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg
    29401 gataacgccg tgcgctacag tccacagggc agcgtgatag acgtcacgct gaatgctgat
    29461 aatttcatcg tgagggataa cggccccggt gtgacgccag aggctctggc gcgaattggc
    29521 gaacgcttct atcgcccccc cggacaaacc gctaccggca gtggacttgg gctatcgatt
    29581 gtccagcgaa tcgccaaatt gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt
    29641 ggatttgagg cgaaggtaag ctggtaactt ctctgtctat gagagccgtt aaaacgactc
    29701 tcatagattt tactaatagc aaaatataaa ccgtccccaa aaaagccacc aaccacaaac
    29761 cagacaaata ccgagcgaaa atacatatac ccaaaactcg aacatttccc gcataaagag
    29821 ttttcttaag ataagaataa taagtggcgt aagaagaaaa aatgctgcaa tcattgcttg
    29881 caatatacga tcttcgcctg aaacggaagg tatactgcaa atattgtctc ccgactcaag
    29941 catccagtca tacttattga aag
//
LOCUS       DAZFVO020000047        29798 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFVO020000047 DAZFVO020000000
VERSION     DAZFVO020000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.047"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            9..1298
                     /gene="uraA"
                     /locus_tag="ACHKNB_RS09755"
                     /old_locus_tag="pgaptmp_001951"
     CDS             9..1298
                     /gene="uraA"
                     /locus_tag="ACHKNB_RS09755"
                     /old_locus_tag="pgaptmp_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HGU4195476.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1393..2094
                     /gene="hda"
                     /locus_tag="ACHKNB_RS09760"
                     /old_locus_tag="pgaptmp_001952"
     CDS             1393..2094
                     /gene="hda"
                     /locus_tag="ACHKNB_RS09760"
                     /old_locus_tag="pgaptmp_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HGU4195477.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2232..2591)
                     /gene="arsC"
                     /locus_tag="ACHKNB_RS09765"
                     /old_locus_tag="pgaptmp_001953"
     CDS             complement(2232..2591)
                     /gene="arsC"
                     /locus_tag="ACHKNB_RS09765"
                     /old_locus_tag="pgaptmp_001953"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HGU4195478.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2612..4075)
                     /gene="bepA"
                     /locus_tag="ACHKNB_RS09770"
                     /old_locus_tag="pgaptmp_001954"
     CDS             complement(2612..4075)
                     /gene="bepA"
                     /locus_tag="ACHKNB_RS09770"
                     /old_locus_tag="pgaptmp_001954"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HGU4195479.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4288..5349
                     /gene="yfgO"
                     /locus_tag="ACHKNB_RS09775"
                     /old_locus_tag="pgaptmp_001955"
     CDS             4288..5349
                     /gene="yfgO"
                     /locus_tag="ACHKNB_RS09775"
                     /old_locus_tag="pgaptmp_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4195480.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5387..6235)
                     /gene="focB"
                     /locus_tag="ACHKNB_RS09780"
                     /old_locus_tag="pgaptmp_001956"
     CDS             complement(5387..6235)
                     /gene="focB"
                     /locus_tag="ACHKNB_RS09780"
                     /old_locus_tag="pgaptmp_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HGU4195481.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(6257..8275)
                     /gene="hyfR"
                     /locus_tag="ACHKNB_RS09785"
                     /old_locus_tag="pgaptmp_001957"
     CDS             complement(6257..8275)
                     /gene="hyfR"
                     /locus_tag="ACHKNB_RS09785"
                     /old_locus_tag="pgaptmp_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HGU4195482.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVTLRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADADAWRSMTDLQESLQQENH
                     QLSEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQ
                     LSPRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEI
                     GDLPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFY
                     RLNVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNV
                     RELENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEE
                     ERQRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8305..8718)
                     /gene="hyfJ"
                     /locus_tag="ACHKNB_RS09790"
                     /old_locus_tag="pgaptmp_001958"
     CDS             complement(8305..8718)
                     /gene="hyfJ"
                     /locus_tag="ACHKNB_RS09790"
                     /old_locus_tag="pgaptmp_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HGU4195483.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8711..9469)
                     /gene="hyfI"
                     /locus_tag="ACHKNB_RS09795"
                     /old_locus_tag="pgaptmp_001959"
     CDS             complement(8711..9469)
                     /gene="hyfI"
                     /locus_tag="ACHKNB_RS09795"
                     /old_locus_tag="pgaptmp_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HGU4195484.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLHDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(9466..10005)
                     /gene="hyfH"
                     /locus_tag="ACHKNB_RS09800"
                     /old_locus_tag="pgaptmp_001960"
     CDS             complement(9466..10005)
                     /gene="hyfH"
                     /locus_tag="ACHKNB_RS09800"
                     /old_locus_tag="pgaptmp_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HGU4195485.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(10015..11730)
                     /gene="hyfG"
                     /locus_tag="ACHKNB_RS09805"
                     /old_locus_tag="pgaptmp_001961"
     CDS             complement(10015..11730)
                     /gene="hyfG"
                     /locus_tag="ACHKNB_RS09805"
                     /old_locus_tag="pgaptmp_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HGU4195486.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11720..13300)
                     /gene="hyfF"
                     /locus_tag="ACHKNB_RS09810"
                     /old_locus_tag="pgaptmp_001962"
     CDS             complement(11720..13300)
                     /gene="hyfF"
                     /locus_tag="ACHKNB_RS09810"
                     /old_locus_tag="pgaptmp_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HGU4195487.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13305..13955)
                     /gene="hyfE"
                     /locus_tag="ACHKNB_RS09815"
                     /old_locus_tag="pgaptmp_001963"
     CDS             complement(13305..13955)
                     /gene="hyfE"
                     /locus_tag="ACHKNB_RS09815"
                     /old_locus_tag="pgaptmp_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HGU4195488.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13967..15404)
                     /gene="hyfD"
                     /locus_tag="ACHKNB_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /pseudo
     CDS             complement(13967..15404)
                     /gene="hyfD"
                     /locus_tag="ACHKNB_RS09820"
                     /old_locus_tag="pgaptmp_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(15421..16368)
                     /gene="hyfC"
                     /locus_tag="ACHKNB_RS09825"
                     /old_locus_tag="pgaptmp_001965"
     CDS             complement(15421..16368)
                     /gene="hyfC"
                     /locus_tag="ACHKNB_RS09825"
                     /old_locus_tag="pgaptmp_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HGU4195489.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16379..18396)
                     /gene="hyfB"
                     /locus_tag="ACHKNB_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /pseudo
     CDS             complement(16379..18396)
                     /gene="hyfB"
                     /locus_tag="ACHKNB_RS09830"
                     /old_locus_tag="pgaptmp_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(18396..19013)
                     /gene="hyfA"
                     /locus_tag="ACHKNB_RS09835"
                     /old_locus_tag="pgaptmp_001967"
     CDS             complement(18396..19013)
                     /gene="hyfA"
                     /locus_tag="ACHKNB_RS09835"
                     /old_locus_tag="pgaptmp_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HGU4195490.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRREPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(19266..19736)
                     /gene="bcp"
                     /locus_tag="ACHKNB_RS09840"
                     /old_locus_tag="pgaptmp_001968"
     CDS             complement(19266..19736)
                     /gene="bcp"
                     /locus_tag="ACHKNB_RS09840"
                     /old_locus_tag="pgaptmp_001968"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HGU4195491.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19736..20308)
                     /gene="gcvR"
                     /locus_tag="ACHKNB_RS09845"
                     /old_locus_tag="pgaptmp_001969"
     CDS             complement(19736..20308)
                     /gene="gcvR"
                     /locus_tag="ACHKNB_RS09845"
                     /old_locus_tag="pgaptmp_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HGU4195492.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20454..21332
                     /gene="dapA"
                     /locus_tag="ACHKNB_RS09850"
                     /old_locus_tag="pgaptmp_001970"
     CDS             20454..21332
                     /gene="dapA"
                     /locus_tag="ACHKNB_RS09850"
                     /old_locus_tag="pgaptmp_001970"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGU4195493.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21349..22383
                     /gene="bamC"
                     /locus_tag="ACHKNB_RS09855"
                     /old_locus_tag="pgaptmp_001971"
     CDS             21349..22383
                     /gene="bamC"
                     /locus_tag="ACHKNB_RS09855"
                     /old_locus_tag="pgaptmp_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HGU4195494.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            22596..23309
                     /gene="purC"
                     /locus_tag="ACHKNB_RS09860"
                     /old_locus_tag="pgaptmp_001972"
     CDS             22596..23309
                     /gene="purC"
                     /locus_tag="ACHKNB_RS09860"
                     /old_locus_tag="pgaptmp_001972"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGU4195495.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23463..23690
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS09865"
                     /old_locus_tag="pgaptmp_001973"
     CDS             23463..23690
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS09865"
                     /old_locus_tag="pgaptmp_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HGU4195496.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23690..24088
                     /locus_tag="ACHKNB_RS09870"
                     /old_locus_tag="pgaptmp_001974"
     CDS             23690..24088
                     /locus_tag="ACHKNB_RS09870"
                     /old_locus_tag="pgaptmp_001974"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4195497.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24235..25098
                     /gene="ypfJ"
                     /locus_tag="ACHKNB_RS09875"
                     /old_locus_tag="pgaptmp_001975"
     CDS             24235..25098
                     /gene="ypfJ"
                     /locus_tag="ACHKNB_RS09875"
                     /old_locus_tag="pgaptmp_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HGU4195498.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25113..27128
                     /gene="tmcA"
                     /locus_tag="ACHKNB_RS09880"
                     /old_locus_tag="pgaptmp_001976"
     CDS             25113..27128
                     /gene="tmcA"
                     /locus_tag="ACHKNB_RS09880"
                     /old_locus_tag="pgaptmp_001976"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HGU4195499.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            27202..27900
                     /gene="ypfH"
                     /locus_tag="ACHKNB_RS09885"
                     /old_locus_tag="pgaptmp_001977"
     CDS             27202..27900
                     /gene="ypfH"
                     /locus_tag="ACHKNB_RS09885"
                     /old_locus_tag="pgaptmp_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4195500.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(28010..28210)
                     /gene="ypfN"
                     /locus_tag="ACHKNB_RS09890"
                     /old_locus_tag="pgaptmp_001978"
     CDS             complement(28010..28210)
                     /gene="ypfN"
                     /locus_tag="ACHKNB_RS09890"
                     /old_locus_tag="pgaptmp_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HGU4195501.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28238..29365)
                     /gene="dapE"
                     /locus_tag="ACHKNB_RS09895"
                     /old_locus_tag="pgaptmp_001979"
     CDS             complement(28238..29365)
                     /gene="dapE"
                     /locus_tag="ACHKNB_RS09895"
                     /old_locus_tag="pgaptmp_001979"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HGU4195502.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29369..29725)
                     /gene="yffB"
                     /locus_tag="ACHKNB_RS09900"
                     /old_locus_tag="pgaptmp_001980"
     CDS             complement(29369..29725)
                     /gene="yffB"
                     /locus_tag="ACHKNB_RS09900"
                     /old_locus_tag="pgaptmp_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HGU4195503.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         7202 a         7772 c         8006 g         6818 t
ORIGIN      
        1 ataatactat gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa
       61 tcccgcttag tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct
      121 tatttcatat taacccggcg actgtactgt tatttaacgg tattggaacg ctgctgtatc
      181 tcttcatctg taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac
      241 cggtattgtt actgttgccg ttagggtatg aagtcgcgct gggcggcttt attatgtgcg
      301 gcgtgctgtt ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg
      361 tgctgtttcc acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg
      421 gcgtagctgc cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa
      481 ccatcatcat ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg
      541 gtttcctggc aattatcccg attttaattg gcgtgctggt gggctacgcg ctctctttcg
      601 caatgggaat tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccaaccc
      661 tctatacgcc gcgcttcgag tggtttgcca ttctgactat tctgccagcg gcgttagtgg
      721 ttattgccga acacgtaggg cacctggtag taacggctaa tatcgtcaaa aaagatctgc
      781 tgcgcgatcc aggtctgcac cgttcgatgt ttgctaatgg cttgtcgacc gtgatctccg
      841 gcttctttgg ctctacgcca aataccactt acggagaaaa cattggcgtg atggcgatca
      901 cccgtgttta cagtacctgg gttatcggcg gggcggcgat tttcgctatc ctgctttcct
      961 gcgtcggtaa actggctgcc gctatccaga tgatcccatt gccggtgatg ggcggcgttt
     1021 cgctgctgct ttatggtgtc atcggtgctt ccggtattcg tgttttgatc gaatcgaaag
     1081 tggattacaa caaagcacag aacctgatcc tgacttccgt gattttgatc atcggcgtca
     1141 gtggcgcgaa ggtaaacatc ggcgcggcgg agctgaaagg tatggcgctg gcgaccatcg
     1201 tcggtatcgg cttaagtttg attttcaaac tgatcagtgt gctgcgccct gaagaagtgg
     1261 tgctggacgc agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc
     1321 tgcccggttc aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg
     1381 gttgaggtat ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac
     1441 gaaacctttg caagtttctg gccgggggat aactcctctt tactggccgc gctgcaaaac
     1501 gtgctgcgtc aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc
     1561 agccatctgc tgcacgcggc ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat
     1621 gtcccgctgg ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg
     1681 tcactggtct gtatcgacaa cattgagtgt attgccggcg atgagttgtg ggagatggcg
     1741 attttcgatc tctacaatcg aattctggaa tcgggcaaaa cacggttgtt gatcaccggc
     1801 gatcgtccac cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg actcgactgg
     1861 gggcagatct acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta
     1921 cgcgcgcgtt tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg
     1981 ctcgacagag aaatgcgcac gctatttatg acgttggatc agttggatcg tgcgtcgatt
     2041 accgcgcaac gtaagctgac cattccgttt gtgaaagaaa ttctgaagtt gtagataagg
     2101 tgtttattgt cggatgcggc gcgaacgcct tatccgaact acgcgaagtt aatccatagt
     2161 attcgtaggc cggataagac gtggcaagcg tcgcatccgg caatgtgctt caactagcga
     2221 tggagcacat cttaacccac gatttccagt acctgctccg gtggacgacc aatccgcgct
     2281 ttgccattcg cgaccacaat cgggcgctcc atcagcttcg gattatccac cattgcctga
     2341 atcagcgcct cttcgctaag cgaactgtca gccaggttca gttctttata aagatcctct
     2401 ttctggcgca tcaattcacg ggcgctattc atccccagca ttttcagcaa atcgcgcagc
     2461 gttgccgcat cggcgggtgt ctcaaggtag agtaccactt ctggctccac gccgttttct
     2521 ttcagcaggt tcagcgtttc ccggctcttt gaacagcgtg ggttatggta gatttttacc
     2581 tgtttggtca ttgttcttcc tttaatgcga attacatctt ggtataaggc ttaaagcgtt
     2641 cctgcagctg gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta
     2701 atttcacctg cgaactggcg ctactcaaca gcgaaatggc ctgatcgagt cgtccggcga
     2761 gcgcataacc ttctgctcgc gcagccagct cctgatcgcg gttatttagc gcggcttccg
     2821 cctgtgccag taaatcccag ccgttgctgt catctttatt attaaaggtg tagcgattca
     2881 gaatattggc cgcttcttgt ggttgaccgc cttgtagata agcgttcgcc aggttgagct
     2941 gcaacaccgg attggtgcgc aaatcacggg catttttcag gcgattgatt gcctcattgg
     3001 ctttgttttg cccaagatcg atatcggtag ccagatcgag ataccatgcg ttgccaggtt
     3061 ctgccgccag taacggttgc agcgttttac gcgcctcgtc gtatttattg gcttccatcg
     3121 cctgtaaagc acgaccatat tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt
     3181 catccagcaa atcactggtg agctggttac gtccggaatt atacatcccc agtgtgcgcg
     3241 ctttcgccag atagaaatct tccgacgact gcaccaccat cggtcgcatc tgattagcac
     3301 ggttgcgggc atctgccaga cgactttccg gcaacgggtg agtcaataaa atttccggcg
     3361 ggcgcgagga gtaacgcgcc tgatcgagta atttttcgag gaaggttggc atcgcctgcg
     3421 gatcgaatcc cgagcgttgc agcacctgaa taccaatgcg atccgcttcc tgttcatttt
     3481 gctgggtgaa gctgatcatc ccctggcgcg ttcccgccag tgtaccggtc agcgctgcca
     3541 tccccgcctg cggactggcc atcgccagta aaatagaacc taacgcgccg acccaggtca
     3601 gcggcgcgct gcgctgttga tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg
     3661 agatttcatg cgccattact gaagccagtt ggctttcgtt atcggaataa cggaacaggg
     3721 cagagtgcag taccacgttg ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga
     3781 tcagaaaaaa atgaaacggt gtcttaaccg aattggcatg cgaaaccaga cgcattccta
     3841 gtgaattaat gtactgcgtt aatagtggat cattaattaa cggcgcgctg ccgcgtagct
     3901 ggcggacata atagtcgccc atctgcattt cctgaccaat ggaaagcgtg cttcctgcgg
     3961 aggttcccat atccggcaag gtgtctgcac tatcggcaaa cgccggggct acctgaccaa
     4021 tggtcatggc agcaatgagg gttgcaacca ggtttttttt caactgcctg aacataacct
     4081 ctatcctgta tttctggaga tagtcatttg accgatactg tgaaatatcg ttcccaccgc
     4141 gccgcctttg agtgtaacgg cgaagattag tccactcaaa tgacttttca gtttcctgaa
     4201 tccgtcataa aaagcgaagt cttttttgtg acatttcgat acaattgcgg atcgcaatcc
     4261 cgctcttgag gttcagggaa ggtctttatg ctcgaaatgt tgatgcaatg gtatcgccgc
     4321 cgttttagcg acccggaagc gattgccttg ctggttattt tagttgccgg atttggcatt
     4381 atctttttct ttagtggcct gcttgctccg ctgttggtgg ctattgtgct ggcctatttg
     4441 ttggaatggc caaccgtgcg cctgcaatct attggctgct cccgccgctg ggcgacgtcg
     4501 attgtattgg tggttttcgt cggtatattg ctattgatgg cgttcgtggt actgcctatc
     4561 gcctggcagc agggcatcta cttaattcgc gatatgccgg ggatgctcaa taagctttct
     4621 gactttgccg ccacgttgcc gcgccgctat ccggcgttga tggatgcggg cattattgat
     4681 gcaatggccg aaaatatgcg cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt
     4741 tccctcgcct cgctggtcgg tttgctgacc attgccgtct atctggtgct ggtaccattg
     4801 atggtcttct tcctgctgaa agacaaagag cagatgctga acgccgttcg ccgggtgctg
     4861 ccgcgtaacc gtggactggc aggacaggtg tggaaggaga tgaatcaaca aatcaccaac
     4921 tatatccgcg gcaaagtgct ggagatgatc gtggtgggga ttgccacctg gctgggattc
     4981 ttactcttcg ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt
     5041 attccgtaca ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc gctattccag
     5101 tttggtgcag gcacggaatt ctggagctgc ttcgcggtgt atctgattat tcaggcgctg
     5161 gacggcaacc tgttagtacc ggtgttgttc tccgaagcgg ttaacctgca tccgctggtg
     5221 attattttat cggtggtgat cttcggtggt ttgtggggat tctggggcgt attcttcgcc
     5281 attccgttgg cgacgctgat caaagccgtg atccacgcct ggcccgatgg gcaaatcgcg
     5341 caagaataat cttcagcccg acgaactgtt tcgccgggct gatttttcag ggttcctgac
     5401 gtaaataaat agcccgataa caaatactca ccagcactgc accgccgata atattaccca
     5461 gcatcaccgg gagcagattg gcggtaataa aatggctgac cgtcagtgcc ggaaaattgt
     5521 ctgcgctact gtgcgccagt tgccagaagg aggtaggggc gaaatggcga atggcaatgg
     5581 cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa gccactggcg acaaacaggg
     5641 tgatgggcaa aatcatggcg acgattttgt cgcataacga acgcccgcaa taactcatcc
     5701 acagcgccag gcaaaccatc agattgcaca taatgccgag gctgacagat tcagtaaatg
     5761 tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac tccccacatc gcgttttcac
     5821 tcatcaccag cccggaaaac cagattaaca aactgaaaca ggcaatacct gccagattac
     5881 cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca actaataacg ccccgacttt
     5941 ttgccattac cgtcattacc gacgaggtga acaggttggt gccgcaaacc gccagcaaaa
     6001 taaagccaag tgtaaagcaa aggccgccca ccagatgggt taatgcctgt gaagacgggg
     6061 catcggcgat aactgaaagg taaaaagtaa atccgatcgc cataaacacg ccagcggaca
     6121 tcgccattaa aaagaccgac actttactgc gggcaatttt atgggcggca atccgttcag
     6181 cgattgccgc ttttctggcg ctcaactgca agtcgaaaga gagtttgttt ctcatgcata
     6241 ctcctgcaaa agcagattac aacacctcgc gaaccgagat tcccagacgc tgcattcgtg
     6301 acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc tccacggggc ccggcaacaa
     6361 tgccattggt ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg
     6421 gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg
     6481 tcggcagtaa acggctttgt cggacattaa gatgtaaatt cagactgtta ccgcgagtca
     6541 gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac gttgcccggc cagtcccacg
     6601 acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc atatggcgcg
     6661 ccattttttg cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg
     6721 gcggcaattc cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat
     6781 cttcaaccat ttgccacaaa tcacggttgg tggcggcaat gactctgaca tttaccggga
     6841 tcgttctact cccgccgaga cgctcaatct cccgttcctg caatacgcgc agcagtttag
     6901 gctgaagttc taacggcaga tcgccaattt catcgagaaa caacgtgccg ccatcggcaa
     6961 tttcaaaacg accacgatgt gtattaatcg caccagtaaa cgcccctttg tcatgaccga
     7021 ataactcact ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct
     7081 tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat cacctctttg ccggttccgg
     7141 tttcaccgca aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagca
     7201 ggtcttccat tgcctggctt tgatagataa tgtcaccgac gcccagattc gaaaggagct
     7261 gctcgctaag ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctgcgcc
     7321 aggcatcggc atcggcattg ccaacggcaa tagcgatgcg atcggctatg tgttgtagta
     7381 gctggcagtt ttcctcacta aagagagtgg aagaggtatg cgccagcaac aatgcacccg
     7441 gtgtatggtt gccaaaggta agcggtatca ggagcgcaga ttcgcagccg ttagatgcaa
     7501 gtaagagtaa taacgggtcg cgttgccaga gaaacagatc atctgcacga tgcgtcaagg
     7561 tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact gtcgccaggc atattgcgtg
     7621 gcagacacgc acaatgtgag gcagaaagat cgctgcacca caggctgaac ttctcgttct
     7681 ttcgatgatc gcccagtacc atactgactg aagccagacc gaaaaaatga tggatctcac
     7741 gggcgacgtc agcgatcaga tcgtcgagat caagatgtga cagcactgca ttagtgatat
     7801 ccaccagtac ccgatgatga tcgcgatctt tctccagcaa acgcagcgtc acctgctgtt
     7861 cttcatcatt gagccatgat tgcagaaccg gggtgataat gcgtgccagc cagttaaaca
     7921 gtgtgcaatc ctggggagta aaacggcagc cgtgggtacg aataaagcgt agctccgcca
     7981 ttccactact gccgagaagg acggcgtgac gcgaataatc gaccatctcg ccctgatact
     8041 ctgttgttgc cgtcacatcg ctgccatcat catggtagcg gtaatagaag ttgcttttga
     8101 gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg cggcagcggg atgaaggcgc
     8161 gtaataaaga cgccttaccg tgtttctgcg ctaaccgctc cgcgagcatt ccgtttaccg
     8221 cttcaattgt tattccttgc ggcggggcaa acatcgcctc gtctgacata gccatcaaca
     8281 gtccttatta atgagaaatc cttctcacgc aatttttctg gccatcagat agatggccgg
     8341 ttcctgcaca atttcatcca acagatgaat gaattgaatg ctacgcgcct gccacacaga
     8401 cgtcgcgtca tccgccagtg gcgcgaatgc ccgggtcaac agggcggtgt ggctggcgtc
     8461 aataacaatc tcaccaaaag tcatcacccc cagcatctta cgtcgggctt gctcctcttc
     8521 gacctgggca agccaatctt cgtattccgt cagtgggcag gggaaggcga cattcagaca
     8581 atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa tacattacct gagagctgtg
     8641 ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc agcgtccaga aaacaatttc
     8701 cccgcactct tcagtcatgt aacccccgga gtacgcgaaa gagttgctga acgatactat
     8761 tgagacgtgg atcgtcggca tcgtgcaacc aggtgttaac ccgatttcct gtaggatcgt
     8821 cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc ctgacgatag cccgccagcc
     8881 gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg gatctgtggc cacaacggtt
     8941 gcatagtcac cccggtggga tcgcgataat ccacagcgtg aatcttctgt tgcagcaaac
     9001 cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt tggcgggcag ccggggatcc
     9061 aaacatcaat ggggacaatg gtgtcgctgc cgccccagac gctgtagaga tcgtggaaaa
     9121 taccgccgcc gacaccgcac gcgccgtagg aaacacagat tttatgatcg ggggcagact
     9181 catacgcccg aagtgcaggc atacgcatcg cccgggtgac tgcgccagta aataacaaaa
     9241 tatcggcgtg acgcggtgat gaaacaacct taatgccaaa acgttctgcg tcgaatactg
     9301 gtgtgatggc agcaaaaatt tcgatttcac aggcgttgca gccgccgcaa tcgacgcgat
     9361 aaacgtaagc cgagcgacgg atatcctgta gcaaatgctg cttcatcttt tgcgtttgct
     9421 cgtccagcgt gatgggctgg ctgacatatt gcgtaagcac tggactcata gctgctcctt
     9481 agccaccagc ggtacatctg tatcgtcgtt gatcagcgtc gcgcgttgtt tgcactccgg
     9541 gcagacgctc gcttgcgccc gcaacatttc gcggttttgt ggcgcatttt gttgctgtgc
     9601 caacaattca gcagccagtg cgacggtttt ttgcggggca aacgggcgtt cgcaacggct
     9661 gcaacgttgt agatggaacg tcgcgcgggt atagagatcg gctttattgg tgacggtcag
     9721 ttcaaagtta ttggtaagct ggatggctct ggtcgggcac acttcttcac aacgtccgca
     9781 gtaaatacaa cgcctcagat agagctgcca ggtgcgcgaa ttttgctggt cgtcggtctg
     9841 gatagtcagc gcatttgccg gacaagcaca ggcgccgcag gcaatacatt ggctgggcat
     9901 caggtccggt ttcccgcgaa agcctgggct gacctccagt ggcgcgaagg gatatttcac
     9961 cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc agcatcttct gtccttactt
    10021 cagcggcgaa cggttacgat caatgccgta gcgttcgatc tctttatacg gcacggtttt
    10081 tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg gtacaggagt agcagggatc
    10141 aaggctaccg ataatcagcg gtgcgtcaga aacggtattg ccgcgcagca tgtaacgcaa
    10201 caccggccag ttggcgtagg tggcggcacg acagcgccag cggaacaatt tttggttatc
    10261 accgagcatg ctccagtgca cgtcttcacc gcgtggcgct tcaacaaagc ccagcgcgaa
    10321 tgcgtgaggt ttatagctaa agccttcggt cagcagcggg gtatccggca tgttgtcgag
    10381 ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg acacggacca tcacgcggga
    10441 gaaaacatcg ccgccggtga aggtaaacag tgtttttgga atattgccgt agtcggcgta
    10501 ggggtgatca aagcgcaaat cacgggcaaa accactgccg cggatcagcg gccctacagg
    10561 gctataatca cgggcgattt gtcggtcgag aatgccaatg ccctgagtgc gttgttccat
    10621 attcggcata gcaagcagca tctctaccag ctccgacacg tcggcgcgca tctcgcgcac
    10681 cagtttcagc gtttgcagac gttgctcttt gagaatatcg cggcgaacac caccaatcag
    10741 attcagaccg taggttttac gcgacccggt cagcaattcc gccatcgtca tcgacttttc
    10801 ccgcacgcgg aaaaattgca taaagccggt atcaaaacca acaaaatggc aggagaggcc
    10861 aaggttgagc aaatgactgt gtagccgttc gacttccagc agaatcgagc gaatggtatg
    10921 cgctcgttgc ggcacctcaa tccccagtgc attttcaacc gagttggtat aggccacact
    10981 gtgggcaaaa ccgcaaatcc cacacacgcg gtccgataag aaggtcactt cgttgtagcc
    11041 catccgcgtt tctgccagtt tctccatgcc gcgatggaca taaaacaggc ggtaatcagc
    11101 atcgacaatt tgctcgccat ccacaaacaa gcggaagtga cccggttcat cggaggtgat
    11161 atgcagcggg ccgacaggga tcacccgcgc atcgctgttg ccctcattga taaacgcata
    11221 cgtttcggaa tctgtcgtcg gttcagggcg cagtcgataa tccatcgcat ctttgcgcag
    11281 cggatgcata tcttccggcc agtcatcggg caacaccagg cgacgctgat ccggcaggcc
    11341 aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg ccccagaccg cggcagggac
    11401 gcgcggtgtg actgacggaa actcccgact gtcggcatcg accagtgcct tcaccacgat
    11461 ccagcatttt tcggcccctt ccattgaaag ggcataataa accgcgtaat gaccgttaag
    11521 tgtccgctcg tcgttgccaa acaggactgg aagccagcca tcatgttgat aataaagata
    11581 atgtacaacg tcaggcagca gattgatttt caccgtaatg gtgacctgtt caggcgtttg
    11641 tcgctcttca tccagcaccg cgccggggaa ctgcgttttc agggcggcga gaatcgcttc
    11701 gccacgattt gacgatgaat taacgttcac tgtgtttctc cgaaacagat gcggtgcctg
    11761 aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag gtcgtgcgtc cctgagagga
    11821 ctatagtgga agcgcccgcc aggatcctga tgacaggttg tggaatatgc gttcccatcg
    11881 ccagcatcat gaccagcaga atcaccattg gcgaggtggt caaccagccg agatcacccc
    11941 ggttaacggc ctgcggcggt ttcgccatta acacccgcgc agccatccgt accaggcccg
    12001 ccagcaccag cgttaacagc aataacagca ggacgataat cagcaggtga ttacgtgcca
    12061 gtcctgcggt aacggtcata aattcgctaa gaaaaatgtt gaagggcggc atccctgcca
    12121 gcgccagcgc accgccgcca aacagcacgg cggtaaatgg catgattttg agcatcccac
    12181 agacgacgtt gagatcgcgc gtgccgtact tgagcagtac attgccggaa ccgcagaaca
    12241 gcagcgtttt tgccagactg tggtttaagg tgtgcagcag cgcggcaaaa attcccagcg
    12301 gcccgccaat gcctagcgcc accgcgacca gccccatgtt ctccacgctg gagtacgcca
    12361 gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc cgccacggca accgacaaca
    12421 tgccgaagat gagcaacaac cggttgggga aatcgctgcc gatggcctgg cagataatga
    12481 tgtaatagcg aattagcacc aacagcgcgc agttcagcaa tacggcggag agcagggcgc
    12541 tgaccggact cggtgcttca ctgtgagcat ccggcagcca ggcgtgcatg ggaaatagcc
    12601 cggttttggt gccaaagcca attagcacaa acacaaaggc caacagcatt aatgttgggt
    12661 caagcaagga cgattgctta agaacctcgc tccagaatat cgccatttct gcctgcggca
    12721 taacgctggc gacgttggcg tataccagca cggtaccgaa cagaccaaaa gcgacaccaa
    12781 cagtacaaat aatgatgtac ttccatgctg cttccagcga tgaacgctga ccgtaaatgc
    12841 ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc cgcccacatc acaatcaggt
    12901 tattgctggt aacaaccagc agcatggtaa acaaaaacag atggaagaaa ccgtagtaat
    12961 cgcacagcgt aacgggggaa agctcgccgt gtgccacttc atgacgcatg tagccaatcg
    13021 agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa ccgcccagac
    13081 catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg gcagtttgga
    13141 ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat acagtcaccg
    13201 tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa aaaagcagcg
    13261 gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc cttcagcgcg
    13321 gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc cagtaacacc
    13381 atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag ttccggtgct
    13441 cgccaggcaa gaagcgccag caccagatgg gagccgtttt ccatcaggca gtaaccaaaa
    13501 atttgccgca ggatattgcg ctggctgaca atgcacagca ggccaagcag aaaatgacct
    13561 aacgctaccg ccagcgccgg tttcagcccg gtagccatcg gtagcttcac gggctgaacg
    13621 acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg ccatcattgc cggaccgaat
    13681 aacgcttttt ccgggatgtt ctggggaata tttcgtgcag cgtaagtcat gattaacggt
    13741 accagcagca ctttggtgat aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg
    13801 aacaggcacg agagagtggc gaaaatagac accagcacca gcgactggca ggcgtaaaat
    13861 ccgcatgaca ggcgatagct tttgacgcta atcacaaaca gcgatgtcag catcatcagt
    13921 cccgccagat tatttacgat catagaaccg gtcattcatc atctccttac tgcaaccagg
    13981 tcgcggcgat tacgctggaa atcagcgaca tcacaatcag tacaatcaac accaggcgca
    14041 ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct cggtttgcca ggcacaacgc
    14101 gaccaaacca gcgaataaac caggcgaaac tggcgaccga ttcaatcatc agcagaatca
    14161 tggcgggcag caggatccag tactccactg acaacgcaaa accggcagca aacagcggga
    14221 atttactgaa gaagccgttg aacggcggca cgccggtaat cgccagcgct gccacgcaga
    14281 aacccacgcc tggcaacggc agggtgtgca atacgccacg cagacgcggc aacaagcgcg
    14341 tgccgcagct gtaactcagc gcacctgcta caaggaaaaa caggctttta gcgaacgcgt
    14401 ggttgacgat gtaggcgatg ctaccctcca gcgccagccg cgagccgaag atggagagcg
    14461 acaagccgaa gaacatccag ccaagttgag tgatggtcga ccaggccagc aaccgcttca
    14521 tatcctgctg tggcaaatac atcagaaagc cataaagaat ggtgaccagt gccatgacca
    14581 tgccaacgcc gccaatcaca tgcgggatat tgccgccgtc gataattgcg cgggcaaaaa
    14641 tgtaaacgcc cactttcacc atcgatgcgg cgtggagata ggcgctgatc ggtgttggcg
    14701 cttccattgc atccggtagc cacgcttgca tcggtagctg ggccgatttc ccccacgcgg
    14761 caaacaggat cccgccataa accagataac gtgcgtcgcc gtgtaactcg ctcattgcgc
    14821 taagcgcaaa cgttccggtc tgcaaaaaca gcgtggcggc ggcaagatac aaccccaacg
    14881 aaccgatatg ggtgataagc aacgctttta gtgctgaacg ctgcgcttta tcgctctggt
    14941 aataactgat caacgcccag gagcagccgc cctaatttca aaaaacaaca actgaccgag
    15001 cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca aaaatgcgta
    15061 ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat aacccgtgga
    15121 gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg tactcacgcg
    15181 atcaatgacc aggccaaaca atgccacatc ccccacgttg accaacgtca acgtgacggc
    15241 aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg tggtcagtgc
    15301 ggcgaacaaa acccccattc ggcggcccga cgttgtggcg aaaacgaaac gaccagtgcg
    15361 ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg tcagtgctcc
    15421 ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc caagccaggt
    15481 cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga ttgaggccag
    15541 tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca ggcaggcgag
    15601 agaaagttct tgcgcgcgcc caaagggcag aaacagggcc acaaacagtg ctgccatcac
    15661 gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg aatattcggt
    15721 cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa tttttcccat
    15781 ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta gcggcgagtt
    15841 ccagcccgtt gccagcgtat tgctgatcat ctcgatatgc gtggaacctg ctatcagcgc
    15901 caataccagc agtgagagaa tgagcatggg ttcgaccaga atgccgagcg tcaactcgcg
    15961 actggcaccg actcccgcaa acgggcttcc ggtatccagc ccggaaagag caaagaaaaa
    16021 acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg caaaagggga
    16081 aacggtaata aaaagtggta gggccatcgc cagcaccagc atgctgctga ttaataccca
    16141 cggcatcagg cggaacatca gacctgaaga tataggcgca acttcctggc gtttaaacag
    16201 tttgtggata tcgcgataat cctgccagat ccccggtccg cggcgggagt gcattcgcgc
    16261 gcgtatctgc cgggaaatac ccgtaaaaag tggggttagc atcagcagaa tcacggcctg
    16321 tgctaacgca aaaatgacca gatatccgtc gcaaagagtt tgtctcatgg tgatttcctt
    16381 agacggcaat agcgattagc agcacaacca gtgcggcaac cacatacagg caatagagac
    16441 gaaagtcgcc gctttgcaga tgctgtattt ctttggccag ccgttgggtg gcgctcacga
    16501 tggggcggat cacccgctca tcccagaagg gttctgtgct ctgagccctg gcggtgacgt
    16561 gcgcaagacc tttatttagc ctcagcgtag ggtcgagctg ttgtcgtaga cgaaatagcg
    16621 cagaaaagac cacacgcagc ggctgcatca cgccattgcc tgacggggcc atcgcatttt
    16681 cccagccgta gccgcatgcc caggcatctc ctgtgcgacg aaacgctgcg cggcgcgaac
    16741 gacaccacag ccagtaaagg ccaggcagca aaggcatcgc cagtagtaac agcaacagta
    16801 atgagggggt gacccgtgta tgaaacgtgc cgggtacaag tgctattccg ctggcgacag
    16861 tgacgggagg ggtattggta aacgcatggg caatatgcat tatcttcggt gccagccaac
    16921 tggcactaag cgcaattaat acgcagagtg ccgcgagcag tagcatggcg acgatcatcg
    16981 tatttggcac ttcctgtgcc tcttcagcgt gtgtactgcg cggcgcacca cagaaagtaa
    17041 taccgtacat tttgacgaag cacattactg ccagcccacc agtgactgcc agcattacca
    17101 tagcaatagg acccgcaagt tgtagcgcta cggcttccac acgacttagt gagaacagcg
    17161 actgccaggt gtaccattcg ctgataaaac cattcagcgg aggaatggct gatatcgcga
    17221 ggcaaccaat caggcatgct gcggctgtcc acggcatccg tttcgccagt gcccccattt
    17281 tttccatgtc gtgggtatgc aaacgcgaaa taatcgcacc cgcgccgaga aatagcagcc
    17341 ctttgaacag cgcatggttg agcagatgaa acagtgcgcc gagcagtcca accacggtga
    17401 gcagcgggtc gtgcagtgac agaccgtcca tcgccacacc gaccgccagc aaaataatgc
    17461 cgacgttttc gacggtactc caggccagca gccgtttgat atcctgttcc gccagtgcat
    17521 acagcacgcc caggagcgcg gagattgcgc cgaccgccat caccagagtg ccccaccaca
    17581 gaggcaaacc cgtttgcgcc agcagatcca tcgctacttt caggatgccg aaaataccga
    17641 ttttgaccat tacgccagac atcaacgccg aagcgtgcga tggtgcggca gggtgagcgc
    17701 gcggcaacca gctgtgcaac ggcatcatcc cggctttcgc gccaaaaccg aaaaaggcca
    17761 gcagaaacac cgccgacgcc agccccggag aaagtgaaag cgtgcgaaac tggcaaaatc
    17821 gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc ataatcagca ccgatccggc
    17881 gtgggcgata aaaaagtaga gcatgccagc gttgatcgac gttttatcct gcctggcaat
    17941 gaccagaaac caggaagaca gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt
    18001 gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc
    18061 cgccgcgcct ttgccctcgt attcgcgcat ataagtcaat gaatagagcg aacaaaccac
    18121 caccagcaag gagataacca gcaccataaa tgccgagagg ctatccattc gcagggttaa
    18181 atcggcaaag tcaaaagggg tggcaaaatg ggcgactaaa gttacaccag catgtaattg
    18241 cgtaattccg ctgatgatgc caatcacgcc gcccaccagc gatgtgatgc cggaaagctt
    18301 aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc agactggcaa acagatagag
    18361 gatcagcgac caggttaata attgcaaggc atccattagt gttgctcctg agtgagggaa
    18421 aggggatcct ccccgccatt ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc
    18481 tgacgttgca ggctatcgtc ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg
    18541 caggccggac cttctggcaa gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca
    18601 cccggttccc ggcgcagcaa cgcatgttgg ggcagcacgt tttcttcgcc gtctttgagt
    18661 gagccttcag cctgaaaaac atattgcgca tgggcattca ccggacggct tcctgaagcg
    18721 ctgattgcgc caaatgggca gaccacggcg caaagcttgc agccaataca gaggctttcg
    18781 ttgagttgga tcgcatcatc cctctgggag atggcattaa ccgggcagac ctgcaggcaa
    18841 ggggcttcct cacagtgatg acacacgaca ggggcagtga gtgttgacgt cttcgccagg
    18901 gccaggcgcg ggtgttgctg taaaccttgc gttttatgca cgtccgaaca ggcagcgaga
    18961 caggtattac atcctgtaca ccacagtggt tcggccacca caaagcggtt catgtcggct
    19021 ccttgaaaat gatatgaaac attgcatttc atattgcata aaggatgcca ttctgattaa
    19081 ctcaatatat tcaataagtt atgtattgac tggattctga acggtacaaa aatgatggtc
    19141 attaacgttc agcaaaaaat ggattcgaca cccgtgtcgc aggggaaagc gagaatgtga
    19201 tcttgatcac gagaagtgag gtatgctggc cgcaagcgca gccagcacgg aatggagcaa
    19261 agtaatcagg cgtgttcttt cagccagttc agcacaacgt cgtggtgatt gctggttttg
    19321 aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa tcaggaagct gatgcgatga
    19381 atgccatcgt aggttttgcc catgaaggac ttttcacccc agacgccgaa ttgttcgcac
    19441 acctggtggt cctcatcaga caggagcgta aagttaagca gctctttttc cgcaaaacgg
    19501 gagagttttt cgggtttatc ggtgctgata cccagcacat caacgcccgc ttttttcaac
    19561 tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac agccgggggt catggctttc
    19621 gggtagaaat aaaccagaac acgctgtccc tggaagtcgg tcaaattaac ttgttctccg
    19681 tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg ctttcagtgg attcattact
    19741 taactccatc ctgttcatca tgttgggaat aattgacgac gttaatactg ccttgtgcat
    19801 tgagttctgt acatagggct ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg
    19861 ggctgtgggc ggttatctga atatgcaact gcgcagcccg ttcattttca gcaggttgcg
    19921 tgcgcgacac cagctccgca atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt
    19981 caattaaatg cggggagtct gccacatcga cctgaaccca gacagatgct ggcattggcg
    20041 gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag atccagttcg gcacctttca
    20101 acggtaacgt tgattcaatc agagtaatgg cattccatga accggaaagc agcataataa
    20161 acgtgaactc ttctcccagc atcgccaggc gactgtcttc aatattacag ccgcaactac
    20221 tgacatgacg ggtgatggtg ttcacaattc cagggcgatc ggcacccaac gcagtgatca
    20281 ccagataatg ttgcgatgac agtgtcaaac tggttattcc tttaaggggt gagttgttct
    20341 taaggaaagc ataaaaaaaa catgcataca acaatcagaa cggttctgtc tgcttgcttt
    20401 taatgccata ccaaacgtac cattgagaca cttgtttgca cagaggatgg cccatgttca
    20461 cgggaagtat tgtcgcgatt gttactccga tggatgaaaa aggtaatgtc tgtcgggcta
    20521 gcttgaaaaa actgattgat tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg
    20581 gcaccactgg cgagtccgct accttaaatc atgacgaaca tgctgatgtg gtgatgatga
    20641 cgctggagct ggctgatggg cgcattccgg taattgccgg gaccggcgct aacgctactg
    20701 cggaagccat tagcctgacg cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg
    20761 taacccctta ctacaatcgt ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg
    20821 ctgagcatac tgacctgccg caaattctgt ataatgtgcc gtcccgtact ggctgcgatc
    20881 tgctcccgga aacggtgggc cgtctggcga aagtaaaaaa tattatcgga atcaaagagg
    20941 caacagggaa cttaacgcgt gtaaaccaga tcaaagagct ggtttcagat gattttgttc
    21001 tgctgagcgg cgatgatgcg agcgcgctgg acttcatgca attgggcggt catggggtta
    21061 tttccgttac ggctaacgtc gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag
    21121 aagggcattt tgccgaggca cgcgttatta atcagcgtct gatgccatta cacaacaaac
    21181 tatttgtcga acccaatcca atcccggtga aatgggcatg taaggaactg ggtcttgtgg
    21241 cgaccgatac gctgcgcctg ccaatgacac caatcaccga cagtggtcgt gagacggtca
    21301 gagcggcgct taagcatgcc ggtttgctgt aaagtttagg gagatttgat ggcttactct
    21361 gttcaaaagt cgcgcctggc aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc
    21421 tgtagttctg actcacgcta taagcgtcag gtcagtggtg atgaagccta cctggaagcg
    21481 gcaccgcttg cggagcttca tgccccggct ggaatgattt tgccggtgac ctccggtgat
    21541 tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta aggcgctgga cattcgtcca
    21601 ccagcccagc cgctggcact ggtttctggc gcgcgtaccc agttcacggg cgataccgct
    21661 tcattgctgg tggaaaatgg tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg
    21721 caggcgaaaa actacaccat cacccaacgt gatgatgctg gtcagacact gaccaccgat
    21781 tgggtacaat ggaaccgtct ggacgaagac gagcagtatc gtggtcgtta tcaaatctct
    21841 gttaagccgc agggttatca gcaggcggtt acggttaaac tgctgaacct ggaacaggcg
    21901 ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca gcacggagat gatgaacgtt
    21961 atttccgccg gtctggataa atctgccact gacgccgcga acgctgcgca aaatcgtgcc
    22021 tccaccacta tggacgtaca aagtgcagct gatgacaccg gtttaccgat gctggtcgta
    22081 cgcgggccgt tcaatgtggt ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg
    22141 aaagtgaccg acagcacccg ttcgcagggt aacatggccg taacttataa gccgctgtct
    22201 gacagcgact ggcaggaact gggcgcgagc gatccaggcc tggcatccgg tgactataaa
    22261 ctgcaggttg gcgatttaga taaccgcagc agcctacagt tcatcgatcc gaaaggtcat
    22321 actctgactc agagtcagaa cgacgcgctg gtatctgtct tccaggctgc gtttagcaag
    22381 taaaaataca gggctggaat catccggccc ttttttctga tatgatacgc aaacgtgtgc
    22441 gtaggcagga aaacgcgatt ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagt
    22501 tcgaataagg cgttcacgcc gcatccgaca aaacatccgg cacaccagac agcaaaagat
    22561 tttaaaacgt taattcacac ccaggagtga taaagatgca aaagcaagct gagttgtatc
    22621 gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga cctgttggtg ctcgaattcc
    22681 gcaatgatac gtcagcaggg gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg
    22741 tgaacaacaa gttcaactac ttcattatga gcaaactggc tgaagcgggt atcccgactc
    22801 aaatggagcg tctgctctcc gataccgaat gtctggtgaa aaagctggat atggtgccgg
    22861 ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag
    22921 aaggtattga gctgaacccg ccgctgttcg atctgttcct gaaaaacgac gccatgcacg
    22981 atccgatggt caacgaatct tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg
    23041 cgcgtatgaa agagctgacc tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg
    23101 ctggtctgat tctggtcgac ttcaagctgg agtttggcct gtacaaaggt gaagtggtac
    23161 tgggcgatga gttctccccg gacggcagcc gcctgtggga caaagaaaca ctggagaaaa
    23221 tggacaaaga ccgtttccgc cagagcctcg gtggcctgat cgaagcctat gaagccgtcg
    23281 cccgccgcct gggtgtacag ctggactgat ttttctggtt gcttgtttct ttagccggag
    23341 ggtactccgg ctaaatgtct ttgatacccc cgctacaata cttctcatta tctccctttg
    23401 acaggaaagt cataccaggc caatatgtat atacataacg tatatcccaa aaaggggggg
    23461 ctatggaaac tactgtattt ttaagtaatc gcagtcaggc cgtcagatta ccgaaagcgg
    23521 tcgctctgcc agaagacgtt aagaaagtag aaattattgc gattggtcgt acaagaatca
    23581 tcactcccgc cggtgagtcc tgggacagtt ggtttgatgg cgaaaatgtg agtgcggatt
    23641 ttatggatat ccgtgatcaa cccgcaatgc aggaaaggga atcattttga tgctgaaatt
    23701 catgctggat accaatatct gtattttcac tattaagaat aaaccagtac atgtcagaga
    23761 gcggtttaac ctgaattctg gccggatgtg tataagttct gtaacattga tggagcttat
    23821 ctatggggca gaaaaaagtc agatgccaga acgcaatttg gcggttattg aaggtttcgt
    23881 atctcgtttg gtagtgcttg attatgatag tgctgctgcg acgcataccg ggcaaatcag
    23941 ggccgagctt gcccggcagg gaaggcctgt tggacctttt gaccagatga tagcgggaca
    24001 tgctcgcagt cgcggtttga ttgtggttac caacaatacc cgagagtttg agcgagtggc
    24061 gggaatcaga ctagaagact ggagctaaca ttctctttta cttctaacat tcgtaaatta
    24121 tataacgtgg catgaagcct taattcctcc gccattttat cccgctttcc tgtggtaatc
    24181 ttcctctgaa ccacctacga tcatttctat aatgaatata ttgttgaggt atctatgcgt
    24241 tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc gcaacagctc tggcggtccg
    24301 tcaatgggcg gtcccggttt ccgtctgccg agcggtaaag gcgggctgat tttactgata
    24361 gtcgtgctgg ttgcaggcta ctatggcgtt gatttaaccg ggttgatgac cgggcagccg
    24421 gtttctcaac aacaatcaac gcggtcaatt agccctaatg aagacgaagc cgcaaaattc
    24481 acctcggtga ttctggcaac cacggaagac acctggggac aacagttcga gaagatgggt
    24541 aagacctatc agcaaccgaa gctggtcatg taccgtggaa tgacgcgcac cggctgcggg
    24601 gctggtcagt ccattatggg gccgttctat tgcccggcgg atggcacagt ttatatcgat
    24661 ctctccttct atgatgacat gaaagacaaa cttggcgcgg atggcgattt tgcccagggg
    24721 tacgttatcg cccatgaagt cggtcatcat gtgcagaaac tgttaggcat cgagccgaaa
    24781 gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtaa accgcttatc tgtgcgtatg
    24841 gaactccagg ccgactgttt tgccggtgtc tgggggcata gtatgcagca gcaaggcgtt
    24901 ctggaaactg gcgatctgga agaggcgctg aatgcggcgc aggccatcgg cgatgaccgt
    24961 ttacaacagc aaagtcaggg gcgagtagta ccagacagtt tcacacatgg cacttctcag
    25021 caacgctaca gctggtttaa acgtggtttc gacagcggcg atccggcaca atgcaatact
    25081 tttggtaaaa gcatttaatt tccggggcag ggatggctga actgactgcg ctttacacat
    25141 taacagcgca aatgaaacgt gaagggatcc gccgcttgct ggtgttgagc ggggaagagg
    25201 gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt acctggcgac tggctgtgga
    25261 tttcgccgca gccagatgct gaaaaccact gttcaccctc ggcgctacaa actttacttg
    25321 ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg ctttgatgcc gctgcctttg
    25381 cagcacttag cggaacgttg aaagcgggaa gctggctggt tttgttactc cctgtatggg
    25441 aagagtggga aaaccaacct gatgccgact cgttgcgctg gagtgattgc cctgacccta
    25501 ttgcgacgcc gcattttgtc cagcatctca aacgcgtact tacggcggat aacgacgcta
    25561 tcctctggcg gcaaaaccag ccgttctcgt tggcgcattt tactccccgt actgactggc
    25621 accccgcgac cggcgcacca caaccagaac aacagcaact cttacagcag ctactgacca
    25681 tgccgccggg cgtggcagcg gtaacggctg cgcgtgggcg cggtaagtcg gcgctggcag
    25741 ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa
    25801 cggatgtact ggcacaattt gcgggcgaga agtttcgctt tattgcgccg gatgccttgt
    25861 tagccagcga tgagcaagcc gactggctgg tggtcgacga agccgcagcc atacctgcgc
    25921 cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt gttaaccact acggtgcagg
    25981 gctacgaagg caccggacgt ggttttttgc tgaaattttg cgctcgcttt ccgcatttac
    26041 accgttttga actgcaacag ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg
    26101 ttagtgaggc actggttttt gacgatgaaa acttcaccca tacaccacaa ggcaatatcg
    26161 tcatttccgc atttgaacaa acgttatggc gaagcgagcc agaaacgccg ttaaaggttt
    26221 atcagttatt gtctggtgcg cactaccgga cttcgccgct ggatttacgc cggatgatgg
    26281 atgcaccagg gcaatatttt ttacaggcgg ctggcgaaaa cgagattgcc ggagcgctgt
    26341 ggctggtgga tgagggggga ttatctcaac aactcagtca ggcggtatgg gcaggttttc
    26401 gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc gcacggtagc aatccactgg
    26461 cagcgacatt gcgtggacgg cgggtcagcc ggatagcagt tcatccggcg cgtcagcggg
    26521 aaggcacagg gcagcaactt attgctggtg ctttgcaata tacgcgtgac ctcgactatc
    26581 tttcggtgag ttttggttac accggggagt tatggcgttt ctggcaccgc tgcggttttg
    26641 tgctggtgcg gatgggtaat catcgtgaag ccagcagcgg ttgctatacg gcgatggcgc
    26701 tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg tgagcattac cgtttacgtc
    26761 gcgatgcgca agctctcgcg cagtggaatg gcgaaacact tcctgttgat ccactaaacg
    26821 atatcgtgct ttctgacgac gactggcttg aactggccgg ttttgctttt gctcatcgtc
    26881 cgctattaac gtcgttaggt tgcttattgc gtctgctaca aaccagtgaa ctggcattac
    26941 cggcgctgcg tgggcgttta cagaaaaatg ccagtgatgc gcagttatgt accacactta
    27001 aacttgcagg ccgcaagatg ttactggtcc gtcagcggga agaggccgcg catgcgctgt
    27061 tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt
    27121 ttcactgact ccttcaattt tctcccatgg tcattgtgag ccagcggcgt aaaattaatg
    27181 acatcagtta aggagttcgc catgaaacat gaccattttg ttgttcaaag cccggataaa
    27241 cctgcgcaac agttgttgct gcttttccac ggtgtcgggg ataacccggt ggcaatgggg
    27301 gaaataggta gctggtttgc accgctgttt cctgatgcgc tggtggtgag tgtcggtggc
    27361 gcggagccga gcggtaatcc ggcggggcgt cagtggtttt cggtgcaggg tattacggaa
    27421 gataatcgcc aggcgcgtgt ggatgcgatc atgccgacgt ttattgaaac ggtgcgctac
    27481 tggcagaaac agagcggagt aggggctaac gccacggcgc tcatcggttt ctctcaaggc
    27541 gcgattatgg cgctggagag cattaaagcc gaaccgggcc ttgcttcacg cgtcattgct
    27601 tttaacggac gctatgcgag cctgccggaa actgcgtcta cagccacgac aattcacctg
    27661 attcatggcg gtgaagatcc ggttatcgat ctggcgcatg cggttgcggc gcaggaagcg
    27721 ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc
    27781 gacaatcgca gtatgcaatt tgccctggat cacttgcgtt ataccattcc gaaacattat
    27841 ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg atgtgattga aatgatgtaa
    27901 aagacagatt acctggcctg ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt
    27961 gccggatgcg gcgttaacgc cttatccggc ctacggttga gcactcagct tacttcttcg
    28021 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
    28081 ggggaagctc cggcttgttg gcgagaaatt tcttatggtc gacgcgcttg agatctttaa
    28141 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
    28201 gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc tgttccatga
    28261 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
    28321 tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg cgagcaataa
    28381 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
    28441 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
    28501 aaagccacca atccaccgta tagcgcagtt gatgtttttc aagcagggca agcacctgcg
    28561 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
    28621 cacccggaat aacattgtta ctgcccgttc ccgcctgaat attagcaatc tgcatactgg
    28681 tcgccgggaa aaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
    28741 gtgctgcgcg atgtactgga ttgtcagcca gatgcgggta ggccacatgc ccctgaacgc
    28801 catgaatggt aaggttgcag gttaacgatc cgcgacgacc atttttcacc acatcaccaa
    28861 ccacttcgat actcgacggt tcgccgacca ggcagtaatc gagacgctca ttacgtgcca
    28921 tcaacgcttc gacgactttt accgtaccgt tgtgggcact ggcttcttca tcagaggtga
    28981 tcagaaatgc cagtcgcccc gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta
    29041 ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccat
    29101 cacgaatggt gggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
    29161 cgtcggtatg cccggcaaag gctaacgttt caccttgccc acgccatgcc caaaaattct
    29221 gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca
    29281 acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
    29341 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc tgataactgg
    29401 aatcactgaa acccagcagc ataggcttac caggcgcgca gagcaatgga cgtttgataa
    29461 ttgcaggcat ttcagtcatt aatgccgccg cagaggccgc atcggtgatt ttattgcggg
    29521 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt cagtaacgct tcccagccta
    29581 attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    29641 aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg gtgtcacaat
    29701 ttttgatgcc gtaaagtgta accataatga atcctttttc gatctatgag aataaatact
    29761 catttaaggg tgaaaacatt ttgcctgcaa atgattat
//
LOCUS       DAZFVO020000048        29649 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFVO020000048 DAZFVO020000000
VERSION     DAZFVO020000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.048"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(50..1879)
                     /gene="glmS"
                     /locus_tag="ACHKNB_RS09905"
                     /old_locus_tag="pgaptmp_001981"
     CDS             complement(50..1879)
                     /gene="glmS"
                     /locus_tag="ACHKNB_RS09905"
                     /old_locus_tag="pgaptmp_001981"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGU4195504.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="ACHKNB_RS09910"
                     /old_locus_tag="pgaptmp_001982"
     CDS             complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="ACHKNB_RS09910"
                     /old_locus_tag="pgaptmp_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGU4195505.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLLDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3762..4181)
                     /gene="atpC"
                     /locus_tag="ACHKNB_RS09915"
                     /old_locus_tag="pgaptmp_001983"
     CDS             complement(3762..4181)
                     /gene="atpC"
                     /locus_tag="ACHKNB_RS09915"
                     /old_locus_tag="pgaptmp_001983"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGU4195506.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4202..5584)
                     /gene="atpD"
                     /locus_tag="ACHKNB_RS09920"
                     /old_locus_tag="pgaptmp_001984"
     CDS             complement(4202..5584)
                     /gene="atpD"
                     /locus_tag="ACHKNB_RS09920"
                     /old_locus_tag="pgaptmp_001984"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGU4195507.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5611..6474)
                     /gene="atpG"
                     /locus_tag="ACHKNB_RS09925"
                     /old_locus_tag="pgaptmp_001985"
     CDS             complement(5611..6474)
                     /gene="atpG"
                     /locus_tag="ACHKNB_RS09925"
                     /old_locus_tag="pgaptmp_001985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGU4195508.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6525..8066)
                     /gene="atpA"
                     /locus_tag="ACHKNB_RS09930"
                     /old_locus_tag="pgaptmp_001986"
     CDS             complement(6525..8066)
                     /gene="atpA"
                     /locus_tag="ACHKNB_RS09930"
                     /old_locus_tag="pgaptmp_001986"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGU4195509.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8079..8612)
                     /gene="atpH"
                     /locus_tag="ACHKNB_RS09935"
                     /old_locus_tag="pgaptmp_001987"
     CDS             complement(8079..8612)
                     /gene="atpH"
                     /locus_tag="ACHKNB_RS09935"
                     /old_locus_tag="pgaptmp_001987"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGU4195510.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8627..9097)
                     /gene="atpF"
                     /locus_tag="ACHKNB_RS09940"
                     /old_locus_tag="pgaptmp_001988"
     CDS             complement(8627..9097)
                     /gene="atpF"
                     /locus_tag="ACHKNB_RS09940"
                     /old_locus_tag="pgaptmp_001988"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGU4195511.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9159..9398)
                     /gene="atpE"
                     /locus_tag="ACHKNB_RS09945"
                     /old_locus_tag="pgaptmp_001989"
     CDS             complement(9159..9398)
                     /gene="atpE"
                     /locus_tag="ACHKNB_RS09945"
                     /old_locus_tag="pgaptmp_001989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGU4195512.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9445..10260)
                     /gene="atpB"
                     /locus_tag="ACHKNB_RS09950"
                     /old_locus_tag="pgaptmp_001990"
     CDS             complement(9445..10260)
                     /gene="atpB"
                     /locus_tag="ACHKNB_RS09950"
                     /old_locus_tag="pgaptmp_001990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGU4195513.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10269..10649)
                     /gene="atpI"
                     /locus_tag="ACHKNB_RS09955"
                     /old_locus_tag="pgaptmp_001991"
     CDS             complement(10269..10649)
                     /gene="atpI"
                     /locus_tag="ACHKNB_RS09955"
                     /old_locus_tag="pgaptmp_001991"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HGU4195514.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11266..11889)
                     /gene="rsmG"
                     /locus_tag="ACHKNB_RS09960"
                     /old_locus_tag="pgaptmp_001992"
     CDS             complement(11266..11889)
                     /gene="rsmG"
                     /locus_tag="ACHKNB_RS09960"
                     /old_locus_tag="pgaptmp_001992"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGU4195515.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11953..13842)
                     /gene="mnmG"
                     /locus_tag="ACHKNB_RS09965"
                     /old_locus_tag="pgaptmp_001993"
     CDS             complement(11953..13842)
                     /gene="mnmG"
                     /locus_tag="ACHKNB_RS09965"
                     /old_locus_tag="pgaptmp_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGU4195516.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVGGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14221..14664)
                     /gene="mioC"
                     /locus_tag="ACHKNB_RS09970"
                     /old_locus_tag="pgaptmp_001994"
     CDS             complement(14221..14664)
                     /gene="mioC"
                     /locus_tag="ACHKNB_RS09970"
                     /old_locus_tag="pgaptmp_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HGU4195517.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14754..15212)
                     /gene="asnC"
                     /locus_tag="ACHKNB_RS09975"
                     /old_locus_tag="pgaptmp_001995"
     CDS             complement(14754..15212)
                     /gene="asnC"
                     /locus_tag="ACHKNB_RS09975"
                     /old_locus_tag="pgaptmp_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HGU4195518.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15364..16356
                     /gene="asnA"
                     /locus_tag="ACHKNB_RS09980"
                     /old_locus_tag="pgaptmp_001996"
     CDS             15364..16356
                     /gene="asnA"
                     /locus_tag="ACHKNB_RS09980"
                     /old_locus_tag="pgaptmp_001996"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGU4195519.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16361..17812)
                     /gene="viaA"
                     /locus_tag="ACHKNB_RS09985"
                     /old_locus_tag="pgaptmp_001997"
     CDS             complement(16361..17812)
                     /gene="viaA"
                     /locus_tag="ACHKNB_RS09985"
                     /old_locus_tag="pgaptmp_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HGU4195520.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17806..19302)
                     /gene="ravA"
                     /locus_tag="ACHKNB_RS09990"
                     /old_locus_tag="pgaptmp_001998"
     CDS             complement(17806..19302)
                     /gene="ravA"
                     /locus_tag="ACHKNB_RS09990"
                     /old_locus_tag="pgaptmp_001998"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HGU4195521.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19525..21393
                     /gene="kup"
                     /locus_tag="ACHKNB_RS09995"
                     /old_locus_tag="pgaptmp_001999"
     CDS             19525..21393
                     /gene="kup"
                     /locus_tag="ACHKNB_RS09995"
                     /old_locus_tag="pgaptmp_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HGU4195522.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21560..21979
                     /gene="rbsD"
                     /locus_tag="ACHKNB_RS10000"
                     /old_locus_tag="pgaptmp_002000"
     CDS             21560..21979
                     /gene="rbsD"
                     /locus_tag="ACHKNB_RS10000"
                     /old_locus_tag="pgaptmp_002000"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HGU4195523.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21987..23492
                     /gene="rbsA"
                     /locus_tag="ACHKNB_RS10005"
                     /old_locus_tag="pgaptmp_002001"
     CDS             21987..23492
                     /gene="rbsA"
                     /locus_tag="ACHKNB_RS10005"
                     /old_locus_tag="pgaptmp_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HGU4195524.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23497..24462
                     /gene="rbsC"
                     /locus_tag="ACHKNB_RS10010"
                     /old_locus_tag="pgaptmp_002002"
     CDS             23497..24462
                     /gene="rbsC"
                     /locus_tag="ACHKNB_RS10010"
                     /old_locus_tag="pgaptmp_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HGU4195525.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGININK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24487..25377
                     /gene="rbsB"
                     /locus_tag="ACHKNB_RS10015"
                     /old_locus_tag="pgaptmp_002003"
     CDS             24487..25377
                     /gene="rbsB"
                     /locus_tag="ACHKNB_RS10015"
                     /old_locus_tag="pgaptmp_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HGU4195526.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25503..26432
                     /gene="rbsK"
                     /locus_tag="ACHKNB_RS10020"
                     /old_locus_tag="pgaptmp_002004"
     CDS             25503..26432
                     /gene="rbsK"
                     /locus_tag="ACHKNB_RS10020"
                     /old_locus_tag="pgaptmp_002004"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HGU4195527.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26436..27428
                     /gene="rbsR"
                     /locus_tag="ACHKNB_RS10025"
                     /old_locus_tag="pgaptmp_002005"
     CDS             26436..27428
                     /gene="rbsR"
                     /locus_tag="ACHKNB_RS10025"
                     /old_locus_tag="pgaptmp_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HGU4195528.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27394..28821)
                     /gene="mdtD"
                     /locus_tag="ACHKNB_RS10030"
                     /old_locus_tag="pgaptmp_002006"
     CDS             complement(27394..28821)
                     /gene="mdtD"
                     /locus_tag="ACHKNB_RS10030"
                     /old_locus_tag="pgaptmp_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HGU4195529.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28844..29536)
                     /gene="yieP"
                     /locus_tag="ACHKNB_RS10035"
                     /old_locus_tag="pgaptmp_002007"
     CDS             complement(28844..29536)
                     /gene="yieP"
                     /locus_tag="ACHKNB_RS10035"
                     /old_locus_tag="pgaptmp_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HGU4195530.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7126 a         7608 c         7569 g         7346 t
ORIGIN      
        1 tataaaaaag ggccaccggt cggcagccct tttttatcta tgatagtgat tactcaaccg
       61 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
      121 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
      181 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
      241 agacatacaa ctgaccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
      301 acagttcgtt gttcggtgca acgacgataa ccggcatatc cgcgtcaatc agcgccagcg
      361 ggccgtgttt cagctcgcct gcagcgtagg cttcagcgtg aatgtaagag atctctttca
      421 gtttcaatgc gccttccagc gcgattgggt actgatcgcc acggcccagg aacagcgcgt
      481 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gacagcatct
      541 gctcaatacg gctcggcagc gcctgcagac catgcacgat gtcatgttca atggaggcat
      601 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
      661 tgaatgcttt agtggatgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
      721 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
      781 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
      841 aggttatcat caggctgtta cgacgcacgg cagatttgcg atagcggaat tcagaggcga
      901 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtaacgggaa accataccgg
      961 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
     1021 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
     1081 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
     1141 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
     1201 cagttttatc gaagatgttt accgaacggc gagtaatttc cgcaatatcg ccctcttcaa
     1261 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
     1321 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
     1381 ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcggg atagcacgca
     1441 gaacagcctc acgcagagta ccgccctgtt tcagctccca gttcaccaga tgggcaatta
     1501 cttcggtgtc tgtttcagaa acgaaggtat agccacgcgc ttttaactct tcgcgcagcg
     1561 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
     1621 gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg agtatgagca atacccgtac
     1681 cgccatgcag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
     1741 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
     1801 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
     1861 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
     1921 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
     1981 cttgtgggag ggagattatg ttatctcctc atcccatgtg accggattac ccggccagaa
     2041 tcactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
     2101 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
     2161 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
     2221 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
     2281 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag ctttcgagcc
     2341 tttacccaga cgcgcttttt tcatctcaac aaagttaccg acgtgtgcac cttccagcaa
     2401 ctcagcacca ggacgcagtc gggcaaacgg gccaatagta caggccgctg ccagattcgc
     2461 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
     2521 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
     2581 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcaaat caaaacgcgc
     2641 cggatcgagc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
     2701 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
     2761 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
     2821 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
     2881 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
     2941 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
     3001 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
     3061 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
     3121 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
     3181 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
     3241 gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
     3301 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
     3361 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
     3421 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
     3481 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
     3541 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
     3601 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
     3661 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
     3721 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
     3781 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
     3841 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
     3901 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
     3961 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
     4021 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
     4081 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
     4141 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
     4201 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
     4261 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
     4321 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
     4381 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
     4441 catccatacc caggatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
     4501 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
     4561 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
     4621 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
     4681 ccgcaggtac gtatactgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
     4741 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
     4801 tacggcccag cagtgcggat acttccgtac ctgccagggt gtaacgatag atgttgtcaa
     4861 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
     4921 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
     4981 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
     5041 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
     5101 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
     5161 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
     5221 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
     5281 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
     5341 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
     5401 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
     5461 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
     5521 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
     5581 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
     5641 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
     5701 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
     5761 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
     5821 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
     5881 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
     5941 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaggc
     6001 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
     6061 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
     6121 gatcattgcg aggtcgcatt gaacgccttt gtcggtccag gtcttcattt ccgccagcag
     6181 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
     6241 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
     6301 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
     6361 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
     6421 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
     6481 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
     6541 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
     6601 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
     6661 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
     6721 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
     6781 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
     6841 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
     6901 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
     6961 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
     7021 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
     7081 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
     7141 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
     7201 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
     7261 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
     7321 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
     7381 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
     7441 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
     7501 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
     7561 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
     7621 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
     7681 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
     7741 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
     7801 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
     7861 tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
     7921 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
     7981 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
     8041 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
     8101 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
     8161 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
     8221 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
     8281 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
     8341 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
     8401 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
     8461 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
     8521 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
     8581 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
     8641 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
     8701 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
     8761 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
     8821 cagctttcgc ttcgtccaga atctgcgagc ggcgtttatt cgcctgctcg atgattacct
     8881 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
     8941 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
     9001 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
     9061 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
     9121 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
     9181 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
     9241 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
     9301 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
     9361 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
     9421 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
     9481 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
     9541 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
     9601 cagctcaccg gcatacatgt taccgaacag tcgcaaacca agtgaaactg gtttggacag
     9661 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
     9721 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
     9781 caggataaat acgcccaatg ccatagacag cgttacgttc acgtccgcag acggaaccac
     9841 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
     9901 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
     9961 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    10021 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    10081 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    10141 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    10201 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    10261 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    10321 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    10381 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    10441 cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    10501 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    10561 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    10621 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    10681 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    10741 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    10801 aaatattttt ccagtgcata caattgcgac ttttctgcta accctgttcg atcatgaaaa
    10861 actgtaaata acgcgtaaac actggtgata aagcgtgctt cagatcacat attgcgcatg
    10921 ttcgcgcaca gcatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    10981 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    11041 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    11101 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgatg
    11161 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ctggttgttg
    11221 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    11281 tttaatcacc accagatgac gttcgccatc cagggctgga acctgaagtt taaccactga
    11341 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    11401 cgcgtagaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    11461 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    11521 cctgctctgt actggttcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    11581 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    11641 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    11701 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    11761 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    11821 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    11881 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    11941 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    12001 aatggagatg gccgcaggcg tgacgccaga aatacgcgaa gcttggccga tagaggctgg
    12061 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    12121 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    12181 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    12241 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    12301 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    12361 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    12421 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    12481 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgtagca tcagacgata
    12541 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    12601 aactagtacg ccgagatacg cctgagaacg tgccggagcc caaccttctt tgtcagcaga
    12661 cagacgggca gcgttaagac cggccagcaa accttgcgca gcggcttctt cgtaaccggt
    12721 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    12781 tttcaggtcg cgaggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    12841 cgcgttttcc atcccctgca tagagcggac gatttgcatc tgcacatcga acggcaggct
    12901 ggtggagata ccgttcggat aaatttcatt agaggtcagt ccttccggtt caaggaagat
    12961 ctgatgctga tttctgtcgg cgaagcgcat aactttgtct tcgatcgacg ggcagtagcg
    13021 tgggccgaca ccttcgatca cccctgcata cattgggcta cgatcgaggt tactgcggat
    13081 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    13141 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    13201 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    13261 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    13321 acggccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    13381 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgcccac
    13441 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    13501 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    13561 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    13621 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    13681 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    13741 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    13801 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    13861 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    13921 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    13981 tgtgatctct tattaggatc gcactgccct gtggataaca aggatccggc ttttaagatc
    14041 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    14101 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    14161 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    14221 ttatttgagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    14281 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    14341 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac tgccaatacc
    14401 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    14461 aggagaaagg ttgtccggaa tatctccggc accgtgggtg gagctgataa tcagccagat
    14521 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    14581 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    14641 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    14701 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgattatggc
    14761 ttgatggtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    14821 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatc
    14881 acttttataa agatgctgta gtggccggtt gtgtaatagg cttcagtgac ttcatcaagg
    14941 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    15001 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    15061 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    15121 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    15181 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    15241 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    15301 ttattgcatg tgtgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    15361 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    15421 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    15481 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    15541 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    15601 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    15661 cgccccgatg aagagcgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    15721 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    15781 atctgggcgg gaattaaagc aaccgaagct gcagttagcg aagagtttgg tttggcaccg
    15841 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    15901 ctcgatgcca aagggcgtga gcgggcgatt gcgaaagatc ttggcgcggt attcctcgtc
    15961 gggattggcg gcaagctgag tgatggtcat cgtcacgacg tgcgcgcacc ggattatgat
    16021 gactggagca ccccgtcaga gctgggccat gcgggtctga acggcgatat tctggtatgg
    16081 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    16141 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    16201 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    16261 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg tgtatggcca
    16321 gctgctgttc gcgagaacgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    16381 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    16441 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    16501 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    16561 ccaccgcgtc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    16621 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    16681 tgccttgtgg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    16741 agcgccggtt ttcagcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    16801 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    16861 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    16921 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    16981 ccagttccgt tatccctagt gtcgccagtt ctggcggcag gagacgtaaa atatcatcgc
    17041 tttgttgcag accatcaacc tgctcaggaa cagttgccgg ttcgcgcacc atggtgcgga
    17101 aggcttccat ctgcgcatcg ttacgcggta ttgatttggc ttcccgggaa cgccccagct
    17161 gttctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    17221 actgatagtc gccacgttta agctgaccgg cgctcatatc ccacagacga ccagctgcgg
    17281 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcctgaactt
    17341 cacttaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    17401 cgatcagact taaacgccaa cgctggagga aaagcgtatg taacgctgaa gtgatcgtgc
    17461 tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg ggaattcaga cgatgcagta
    17521 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    17581 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    17641 gcagcgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagt cgtgggaatt
    17701 tttcaaagaa gactgccagc tgcggtgagg ccagcagcgc gatgatcatc tcttcgatca
    17761 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    17821 ctggcgaatc tgtgcgccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    17881 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcact
    17941 ttcaagctct tccagttgtt gttttatttc acctggcaga ccttcagccg atgaaccggg
    18001 aagtgccagc gtactgcctt gtaaacttac atcgcgtaca acaagatgtt gggcgctatc
    18061 cacttccaga ttcaatttct gggcaaagcc gataccgttc agtttgccgc gaatttcacc
    18121 acctttactc agccactgtt ccagcgcgtt acgctcaaag gagatatgaa ccacttccat
    18181 atcatgcaat tttaacggtt tttgcagcag cagagtcaga gtggaagcag taacgttaac
    18241 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    18301 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    18361 agtcaacatc ccttgctgtt gccaggcgtg accggtcatc aatacatcaa tttgttgttg
    18421 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    18481 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    18541 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gctggcgcag
    18601 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    18661 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    18721 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    18781 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gcagctcgtt
    18841 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    18901 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    18961 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    19021 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    19081 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    19141 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    19201 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    19261 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
    19321 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
    19381 tcaaaggggg ggtatttttc tttgagccag gttaatgtgg ccgcatttag gagtacgatt
    19441 ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc aagggactaa gcacacattt
    19501 catatttcaa cgaaagacta gtctatgagc actgataata agcaatcatt gcccgcgatt
    19561 accctcgcgg cgattggagt tgtctacggc gatattggta ccagcccgtt atatacactt
    19621 cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt gtttggcttt
    19681 ttatcgctga tcttctggct gctaatcttt gtggtttcca ttaaatatct caccttcgtg
    19741 atgcgggcag ataacgccgg tgaggggggg atcctgacgt tgatgtcgct tgccgggcgt
    19801 aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg cggcagcttt
    19861 ttctatggtg aagtcgtcat aacacccgct atttcggtga tgtcagccat tgaaggtctg
    19921 gaaatcgtcg ccccgcagct ggatacctgg atagttcccc tctcaattat cgttctcact
    19981 ttattattta tgattcaaaa acatggcacg gctatggtcg gtaagctgtt tgcgccgatc
    20041 atgctgacct ggtttttgat tctggcagga ctggggttac gtagcattat tgctaacccg
    20101 gaagtgctgc atgcgctgaa tccaatgtgg gcggtgcatt tcttccttga atacaaaacg
    20161 gtttctttta ttgcattagg ggcagtggtg ttgtcgatta cgggggtcga ggcgctgtat
    20221 gctgatatgg ggcactttgg taagttccct attcgcctgg cgtggttcac cgtcgtatta
    20281 ccttccttga cccttaatta cttcggtcag ggagcgctgt tgttaaagaa cccggaagcg
    20341 attaagaacc cgttcttcct gttggcaccg gactgggcgc tgatcccgct gctgatcatc
    20401 gccgcactgg cgacggtaat cgcctcgcag gctgtcatct ctggcgtctt ctcattgacg
    20461 cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca ttattcacac ctccgaaatg
    20521 gagtcagggc aaatctatat tccgtttgtg aactggatgc tctatgtcgc ggtcgtgatt
    20581 gtgattgtca gctttgagca ctccagcaac ctggcggcgg cgtacgggat tgcggtgacc
    20641 ggaaccatgg tgctgacgtc tattctctcg actactgtgg cacgtcagaa ctggcactgg
    20701 aataagtatt ttgttgcgct gatcctgatt gctttccttt gtgtcgatat tccattgttc
    20761 accgctaacc tcgataaact gctctccggc ggctggttgc cattgagcct cggtactgtg
    20821 atgtttatcg tgatgaccac ctggaagagc gagcgtttcc gcttgctgcg gcggatgcat
    20881 gaacatggta actctctgga agcgatgatt gcttcgctgg agaaatcacc gcctgttcgc
    20941 gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg tcattccctt tgcgctgatg
    21001 cataacctta aacataacaa ggtattgcat gagcgggtga ttctgttaac tctgcgcacc
    21061 gaagacgcgc catatgtcca taacgtccgt cgggtacaga tagaacaact gtcgcccact
    21121 ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc caaacgtgga agaagttttc
    21181 caccgctgcg gtctggaagg attaagttgc cggatgatgg aaacctcctt ctttatgtcg
    21241 catgagtcgt tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg caagctgtac
    21301 ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat ttgaaattcc gccaaacagg
    21361 gttatcgaac tgggtactca ggtcgaaatc taacgccaga cgcctccttt ctccataagg
    21421 gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg atcacatttc
    21481 cgtaacgtca cgatggtttt cccaactcag tcaggattaa actgcgggtc agcgaaacgt
    21541 ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata tttcatcgat
    21601 gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt tacccatccc
    21661 caaaagtaca acgcgtatcg atatggcatt aacccagggt gtaccttctt ttatgcaggt
    21721 gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg aagagatcaa
    21781 acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc tgcaaaaaca
    21841 ccagggaaat accattgaaa ttcgttacac cacgcatgaa caattcaaac aacaaaccgc
    21901 agaaagtcag gcggtaattc gcagcggaga atgttctccg tatgcgaata tcattctctg
    21961 tgctggcgtg acgttctgag gccgtcatgg aagcattact tcagcttaaa ggcatcgata
    22021 aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat ccgggccgcg
    22081 tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa gtgcttactg
    22141 gtatctatgc tcgcgatgcc ggcacgcttt tatggctggg gaaagaaacg acatttaccg
    22201 ggccgaaatc ttcccaggaa gccgggattg ggattatcca tcaggaactg aacctgatcc
    22261 cgcagttgac cattgccgaa aacattttcc tcggtcgtga gtttgttaat cgctttggca
    22321 aaattgactg gaaaaccatg tatgccgaag cggataaatt gctggctaaa cttaacctgc
    22381 gctttaaaag cgacaagctg gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa
    22441 tcgccaaagt gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc
    22501 tgaccgatac cgaaaccgaa tccctgttcc gcgtcatacg cgagctgaaa tcgcaaggcc
    22561 gcggtattgt ctatatctcc caccgcatga aagaaatctt cgagatttgc gatgacgtta
    22621 ccgtttttcg tgacgggcaa tttattgctg agcgcgaagt ggcatcactg accgaagatt
    22681 cgctgattga gatgatggtg ggccgcaagc tagaagatca atatccacac ctggacaaag
    22741 cgccgggaga tatccgcctg aaagtcgata atctctgcgg acctggcgtt aacgatgtct
    22801 cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta
    22861 ccgaactgat gaaagtgctc tacggcgcac taccgcgcac cagcggttac gtcaccctgg
    22921 atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaagcggc attgtgtata
    22981 tctccgaaga ccgtaaacgt gacggtttag tgttgggcat gtcagtaaaa gagaacatgt
    23041 cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac
    23101 agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacaccgtcg atggaacagg
    23161 caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga
    23221 cacgccccaa agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc ggcgcgaaaa
    23281 aagagatcta tcaactgatt aaccagttca aagccgatgg cttgagcatc attctggtgt
    23341 catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg catgaagggc
    23401 atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg gctgccgctg
    23461 tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac tgtctctggt
    23521 cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc tctgctggtg
    23581 ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa cttattcaat
    23641 attctccagc aaacctcggt gaacgccatt atggcggtcg ggatgacgct ggtgatcctg
    23701 acgtcgggca tcgacttgtc tgtaggttct ctgttggcgc taaccggcgc agttgctgca
    23761 tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgctgctct cgcgttaggt
    23821 gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgttca ggcgtttatc
    23881 gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac caacggtagc
    23941 ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg tattggtcgt
    24001 ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc ggcctggtac
    24061 atgctgcatc acacgcgtct ggggcgttac atctacgcgc tgggcggcaa cgaagcggca
    24121 acgcgtcttt ctggtatcaa catcaataaa atcaaaatca tcgtctattc tctttgtggt
    24181 ctgctggcat cgctggccgg gatcattgaa gtggcgcgtc tctcctccgc acaacccacg
    24241 gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg tacgagtctg
    24301 gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct tggcttcctt
    24361 aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt caaagcggtg
    24421 gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact acaggacatc
    24481 ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct aagcgccacc
    24541 gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac gcttaacaac
    24601 ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct tggctataac
    24661 ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt gcaggactta
    24721 accgttcgcg gcaccaaaat tctgctgatt aacccgaccg actccgacgc agtaggtaat
    24781 gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg ccaggcaacg
    24841 aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa aatcgccggt
    24901 gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca aggcattgct
    24961 ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc tgctcacaag
    25021 tttaatgttc ttgccagcca gccagcagat tttgatcgca ctaaaggttt gaacgtaatg
    25081 cagaacctgt tgaccgctca tccggatgtt caggctgtat tcgcgcagaa tgatgaaatg
    25141 gcgctgggcg cgctgcgcgc actgcaaact gccggtaaat cggatgtgat ggtcgtcgga
    25201 tttgacggta caccggatgg cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc
    25261 gctcagctac ccgatcagat tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa
    25321 ggcgagaaag ttcaggctaa gtatccggtt gatctgaaac tggttgttaa gcagtagttt
    25381 taatcaggtt gtatgacctg atggtgacat aaatacgtca tcgacagatg aacgtgtaat
    25441 ataaagaaaa gcagggcacg cgccacccta acacggtggc gcattttatg gacatcccga
    25501 atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat taatgctgac cacattctta
    25561 atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg taaccactat caggttgcat
    25621 ttggcggcaa aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg aatatcgcgt
    25681 ttattgcctg tacgggtgat gacagcattg gtgagagcgt tcgccagcag ctcgccactg
    25741 ataacattga tattactccg gtcagcgtga tcaaaggcga atcaacaggt gtggcgctga
    25801 tttttgttaa tggcgaaggt gagaatgtca tcggtattca tgccggcgct aatgctgccc
    25861 tttccccggc gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca gcattattaa
    25921 tgcagctgga atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc catcaaaata
    25981 agactatcgt tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa ctgctggcgc
    26041 tggtggacat tattacgcca aacgaaacgg aagcagaaaa gctcaccggt attcgtgttg
    26101 aaaatgatga agatgcagcg aaggcggcgc aggtactgca tgaaaaaggt atccgtactg
    26161 tactgattac tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa ggtcagcgcg
    26221 ttcctggatt ccgggtgcag gctgtcgata ccattgctgc cggagatacc tttaacggcg
    26281 cgttaatcac ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg tttgcccatg
    26341 ctgccgctgc gattgccgta acacgtaaag gcgcacaacc ttccgtaccg tggcgtgaag
    26401 agatcgacgc atttttagac aggcagaggt gacgcttggc tacaatgaaa gatgttgccc
    26461 gcctggcggg cgtttctacc tcaacagttt ctcacgttat caataaagat cgcttcgtca
    26521 gtgaagcgat taccgccaaa gttgaagcgg cgattaaaga actcaattac gcgccatcag
    26581 ccctggcgcg tagcctcaaa ctcaatcaaa cacataccat tggcatgttg atcactgcca
    26641 gtaccaatcc tttctattca gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg
    26701 gttatagtct cgtcctttgc aataccgaag gcgatgaaca gcggatgaat cgcaatctgg
    26761 aaacgctgat gcaaaaacgc gttgatggct tgctgttact gtgcaccgaa acgcatcaac
    26821 cttcgcgtga aatcatgcaa cgttatccga cagtgcctac tgtgatgatg gactgggctc
    26881 cgttcgatgg cgacagcgat cttattcagg ataactcgtt gctgggcgga gacttagcaa
    26941 cgcaatatct gatcgataaa ggtcataccc gtatcgcctg tattaccggt ccgctggata
    27001 aaactccggc gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca
    27061 acattcctga tggctatgaa gtcactggtg attttgaatt taacggcggg tttgacgcca
    27121 tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta
    27181 tggctgttgg cgtttaccag gcgttatatc aggcagagtt acaggttccg caggatatcg
    27241 cggtgattgg ctatgacgat atcgaactgg caagctttat gacgccacca ttaaccacta
    27301 tccaccaacc gaaagatgaa ctgggggagc tggcgattga tgtactcatc catcggataa
    27361 cccagccgac ccttcagcaa caacgattac aacttactcc gattctgatg gaacgcggtt
    27421 cggcttagat ttacgctgtc ttttgatcaa attattacca tcggttgttt tcagaagcat
    27481 gaacattgct gctgaagcaa cagtaataat gcccatcgtg ataaacgtat agtggaattg
    27541 ttcgacagtc gttgtgcctt ccatcccttc ataaacgcga aggacggccg cacttacagc
    27601 aacgcctaaa ctaatcgaca gttgctgcgt gaccgccaga acactgttac cgctgctggc
    27661 gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc gcggtaaatt gcgtcgacat
    27721 agccatccct aatataaaca acggcaagat cagcatccat atcgccattg ccggtgattg
    27781 caaagagaac tgagcgatca ttagcccaat aatcaccgtg atccccacta acgtatggcg
    27841 atagcccaga cgacgtaaga cttgggtaac catcgatttt gcaataatgg aacctaacgc
    27901 tgtcggtgcc atcatacagc cggcaataaa cgcctgataa ccaaatccta cctgtaacat
    27961 caatggcata aggaacggta cacaaccggt ccccagacgg gttgcaatat tgcctacgat
    28021 accgatcgag aaagtgcggg ttttaaataa atctaatgaa attaatgggt ttggcgtgtg
    28081 tcgcgcatgg agaatataga gaagcagtaa cccgatgctg gtgacaatta ccgtcaaggc
    28141 aatccagctg gcgacaatct tttccccgaa tagctctatt ccgcttgaga agagaacaag
    28201 gctgaggcca aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg tggtgaaatt
    28261 gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat tgattaaaaa
    28321 tatccagtgc caggttgccc aggtgaccag cacgccgcca agaacggggc ctaaaattgg
    28381 ccccaccaga cccggcatgg cgacaaaatt caatactgga agaagttcat tacgaggata
    28441 agcgcgcagt aaggccagcc gagcaacagg catcatcatt gcgccgccta tcccctgaat
    28501 aacccggaag acaaccagct gtggtagcga attagaaagt gcgcaggcca gagaacccaa
    28561 tgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat cggctagcca
    28621 tccgcttacc ggaataagca tcgccaccgt cagcgtataa ctgatgatgg ctgattgcat
    28681 cgcgagagga gaacgattaa ggctatgagc gattgcgggt aaggcggtat tcagaatagt
    28741 ggcatcaagt gcctgcatga agaaggccat cgccgcgatc cacggcaaac ccgccatact
    28801 gcgattcttt ttatcgctca ttcaatgtcc tgttatcggg ttgtcactta tcaggtgagc
    28861 gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc gctttggaca atcgcatcga
    28921 caatcgcctg gtgcagatcc agctttatca ctgtgtcgct ggtaattgac gtgaagtaag
    28981 tgtgataaac cgaatggaat agcgaggcga atgaggtcaa aaacggattg gcgctcattt
    29041 catagatatg ctcatgccag gccatatcga cttcgatcca gcgttcacgg cgaaagttct
    29101 cttttaatgc cgccatttcg gccattaacg tattgagatg cgccttctgt tccgcggtgc
    29161 caaccgttgc agccagtagg caagcttgcg gctccagaca gatacgcata accagaaagt
    29221 gatcgatgac ctgatgaaag ttctcttctg tcatccacca ggtaagcaat tcctgatcaa
    29281 gaaaattcca gtttgattgt ggcatgaccc gggtaccaat tcgcggtcgc ggtaaaacca
    29341 tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc actccaaatt
    29401 gctcgcccag ctcaatctca ccaggcaaaa tggtgccggg ttcatattca ccttttaaga
    29461 tgcgttgcgc cagcttctca gccagaacat acgaaaggtt tttctgtgca gctaactgtt
    29521 gtgcgcttaa aggcattact aatcttcctt tttcttttta ttcctcctta gtatgccacc
    29581 aggaagtgtg attacggttg caaaaacggc aaattgcttg ttttatggca cattaacggg
    29641 gcttttgct
//
LOCUS       DAZFVO020000049        29252 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFVO020000049 DAZFVO020000000
VERSION     DAZFVO020000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 29252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.049"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..287)
                     /locus_tag="ACHKNB_RS10040"
                     /old_locus_tag="pgaptmp_002008"
     CDS             complement(<1..287)
                     /locus_tag="ACHKNB_RS10040"
                     /old_locus_tag="pgaptmp_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4195531.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSL"
     gene            complement(288..593)
                     /gene="ccdB"
                     /locus_tag="ACHKNB_RS10045"
                     /old_locus_tag="pgaptmp_002009"
     CDS             complement(288..593)
                     /gene="ccdB"
                     /locus_tag="ACHKNB_RS10045"
                     /old_locus_tag="pgaptmp_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGU4195532.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(595..813)
                     /gene="ccdA"
                     /locus_tag="ACHKNB_RS10050"
                     /old_locus_tag="pgaptmp_002010"
     CDS             complement(595..813)
                     /gene="ccdA"
                     /locus_tag="ACHKNB_RS10050"
                     /old_locus_tag="pgaptmp_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGU4195533.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            1660..2898
                     /locus_tag="ACHKNB_RS10055"
                     /old_locus_tag="pgaptmp_002011"
     CDS             1660..2898
                     /locus_tag="ACHKNB_RS10055"
                     /old_locus_tag="pgaptmp_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4195534.1"
                     /translation="MEVFMKDVSILNEMIFSASTENELQEKFKDIYHRINNESEYDDS
                     GSVEFLPLPRLNSRSERSEVQDIISVVIESGFFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHRIARKADIPVIVDAAQCFGIRSLINHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSRMDN
                     LQAATLSVKLHHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPILLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFSDSEKIHSALLHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            2882..3711
                     /locus_tag="ACHKNB_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /pseudo
     CDS             2882..3711
                     /locus_tag="ACHKNB_RS10060"
                     /old_locus_tag="pgaptmp_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            3711..4727
                     /locus_tag="ACHKNB_RS10065"
                     /old_locus_tag="pgaptmp_002013"
     CDS             3711..4727
                     /locus_tag="ACHKNB_RS10065"
                     /old_locus_tag="pgaptmp_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4195535.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVNPDKVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            4729..4998
                     /locus_tag="ACHKNB_RS10070"
                     /old_locus_tag="pgaptmp_002014"
     CDS             4729..4998
                     /locus_tag="ACHKNB_RS10070"
                     /old_locus_tag="pgaptmp_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGU4195536.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            4995..6527
                     /locus_tag="ACHKNB_RS10075"
                     /old_locus_tag="pgaptmp_002015"
     CDS             4995..6527
                     /locus_tag="ACHKNB_RS10075"
                     /old_locus_tag="pgaptmp_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4195537.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWTPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESTRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPLAERFTGEGAIEFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(6560..7012)
                     /locus_tag="ACHKNB_RS10080"
                     /old_locus_tag="pgaptmp_002016"
     CDS             complement(6560..7012)
                     /locus_tag="ACHKNB_RS10080"
                     /old_locus_tag="pgaptmp_002016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGU4195538.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
     gene            7341..7598
                     /locus_tag="ACHKNB_RS10085"
                     /old_locus_tag="pgaptmp_002017"
     CDS             7341..7598
                     /locus_tag="ACHKNB_RS10085"
                     /old_locus_tag="pgaptmp_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195539.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDR"
     gene            7598..8188
                     /locus_tag="ACHKNB_RS10090"
                     /old_locus_tag="pgaptmp_002018"
     CDS             7598..8188
                     /locus_tag="ACHKNB_RS10090"
                     /old_locus_tag="pgaptmp_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195540.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEEAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAERR
                     AAEVVVLPLVPATGLPADGTSATDLAVELNGMTYQACRGDFVVRLDGSTCLQLWNKEG
                     KVVRREGDPLEVAQWLQACHDAGMEVRVQVNESITP"
     gene            complement(8206..8424)
                     /locus_tag="ACHKNB_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /pseudo
     CDS             complement(8206..8424)
                     /locus_tag="ACHKNB_RS10095"
                     /old_locus_tag="pgaptmp_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            8582..8782
                     /locus_tag="ACHKNB_RS10100"
                     /old_locus_tag="pgaptmp_002020"
     CDS             8582..8782
                     /locus_tag="ACHKNB_RS10100"
                     /old_locus_tag="pgaptmp_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195541.1"
                     /translation="MKLDISVKYFLNIMIADFIIPAPEKISTTWHSDTKMNRFACPIH
                     STNSDYYSGFYHTDVLIPGELA"
     gene            8822..9004
                     /locus_tag="ACHKNB_RS10105"
                     /old_locus_tag="pgaptmp_002021"
     CDS             8822..9004
                     /locus_tag="ACHKNB_RS10105"
                     /old_locus_tag="pgaptmp_002021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195542.1"
                     /translation="MIFNWNVLFYVSLEKFPVGNPYYGEIPSIPVDEEAKFFNPFPPR
                     AFENKPLYTEEITKYD"
     gene            8997..9863
                     /locus_tag="ACHKNB_RS10110"
                     /old_locus_tag="pgaptmp_002022"
     CDS             8997..9863
                     /locus_tag="ACHKNB_RS10110"
                     /old_locus_tag="pgaptmp_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4195543.1"
                     /translation="MIKGIMFAFLAAFSWGAAIVMSKKGLENMDAGELFFWQVGSAAL
                     LSWFVLAISRKKLPVTKKSTLAYSTGIFEPFLAYTFTLYGLKFISAGITSVIFSLESV
                     FILILSVIFFSVKINSLLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTDTSLQLPIESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            10104..10289
                     /locus_tag="ACHKNB_RS10115"
                     /old_locus_tag="pgaptmp_002023"
     CDS             10104..10289
                     /locus_tag="ACHKNB_RS10115"
                     /old_locus_tag="pgaptmp_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195544.1"
                     /translation="MLTQLHRVLYRCPGHYVYGSATQSTGTGTGSLASRNTSQSPLPA
                     QQAGFPRPELYSLSVCQ"
     gene            10347..10952
                     /locus_tag="ACHKNB_RS10120"
                     /old_locus_tag="pgaptmp_002024"
     CDS             10347..10952
                     /locus_tag="ACHKNB_RS10120"
                     /old_locus_tag="pgaptmp_002024"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4195545.1"
                     /translation="MTLYQIKPSFQALLRPLMFWLNRRGVTANHITLCAMVLSLITGV
                     VLIIHPQPGLFVLLPVVLFVRMALNALDGMLARECNQRSRLGAILNETGDVISDISLY
                     LPFMLLPQSSIPLVLIVVFCALMTEFCGVLAQTINGVRSYAGPLGKSDRALVFGAWGL
                     SLSIWPQIVAWNNVIWSVATLLLLWTIFNRCRSALREEGVK"
     gene            10949..11866
                     /locus_tag="ACHKNB_RS10125"
                     /old_locus_tag="pgaptmp_002025"
     CDS             10949..11866
                     /locus_tag="ACHKNB_RS10125"
                     /old_locus_tag="pgaptmp_002025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGU4195546.1"
                     /translation="MTLLEKSLVVIFSLLLIATVINGLLVWLRPGKDWRELTLRIRTW
                     WVIIVLFSFALLSPSWLALTFFALVSFMALKEFLTLVPSRQSDRMPLLWMFIAIPLNY
                     WLIGIGWYGMFVVFIPVYVFLFLPARMVIAGDTQGFLRTVSQLHWSLMTTVFAFSHVA
                     FLLVLPADGMQTSALLVLFLVGLTEFNDIAQYLWGKSLGRIKVIPKVSPNKTLEGLLG
                     GVATTALAATVLGPLLTPLSWPMALLAGVIIGVTGFCGDVVMSAIKRDIGVKDSGTLL
                     PGHGGILDRLDSLIFTAPVFFHFVRYFCY"
     gene            11870..12511
                     /locus_tag="ACHKNB_RS10130"
                     /old_locus_tag="pgaptmp_002026"
     CDS             11870..12511
                     /locus_tag="ACHKNB_RS10130"
                     /old_locus_tag="pgaptmp_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGU4195547.1"
                     /translation="MMTRLLRTLFTLCIVWPVIWLWLGLRIRHRERLPKHGPSIIVAN
                     HNSHMDVFALLSLFPLRYQASVHPVAAADYFLRDKRMAWFALKILNIIPISRKGGYTD
                     PLAQCEQALREGKTLILFPEGTRGEPGVLAPLKSGVWHLSQRMPEVPIIPVGLRGTEH
                     VMAKGNRIPLPLFIDVNIGDALSTHPEKKTFMDDLEQRLTALLQPSQGNHPHD"
     gene            12504..14264
                     /locus_tag="ACHKNB_RS10135"
                     /old_locus_tag="pgaptmp_002027"
     CDS             12504..14264
                     /locus_tag="ACHKNB_RS10135"
                     /old_locus_tag="pgaptmp_002027"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HGU4195548.1"
                     /translation="MTNSRIPDERQFLTSDNTELFYRHWPAQEASSTPKVVVLFHRGH
                     EHSGRLQHIVDELAMPDTAFYAMDARGHGRSPGLRGYSPSLVRSVQDVDEFVRFAAAD
                     SQAGMTDVVVVAQSVGAVLAATWAHDYAPDIRGLVLASPAFKVKLYVPFARQGLELMY
                     RLRGLFFVNSYVKGKYLTHDPERVASFNKDPLITRPIAVNILLDLYKTAERVVSDASA
                     ITLPVQLLVSGEDYVVHRQPQLDFYQNLRNPQKELHVLPGFYHDTLGEKERHLAFDRM
                     RSFIDTLYARPVQRFDYSQEDRWSPGADTWRILSGGPALYSLDDISYRCLRYGMKVLG
                     TQSTGVRLGFETGFDSGSTLDYIYRNQPQGSTSLGRLIDKNYLNSVGWKGIRQRKIHL
                     QMLIQQAVARLTEQGSAVRIVDIAAGHGRYVLDALQGETAVSEILLRDYSELNVAKGQ
                     EMIASRGMAGRARFEQGDAFNRNELAALEPRPTLGIVSGLYELFPENGPVRDSLAGLA
                     AAIEPGGILIYTCQPWHPQLKTIAWSLTSHKNGKAWVMRVRTQGEMDALVRDAGFEKC
                     TQLIDEMGIFTVSMAVRRQP"
     gene            14261..14443
                     /locus_tag="ACHKNB_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /pseudo
     CDS             14261..14443
                     /locus_tag="ACHKNB_RS10140"
                     /old_locus_tag="pgaptmp_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14476..15956)
                     /locus_tag="ACHKNB_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /pseudo
     CDS             complement(14476..15956)
                     /locus_tag="ACHKNB_RS10145"
                     /old_locus_tag="pgaptmp_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(15987..16337)
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS10150"
                     /old_locus_tag="pgaptmp_002030"
     CDS             complement(15987..16337)
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS10150"
                     /old_locus_tag="pgaptmp_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4195549.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRRNSLTML"
     gene            complement(16334..16696)
                     /locus_tag="ACHKNB_RS10155"
                     /old_locus_tag="pgaptmp_002031"
     CDS             complement(16334..16696)
                     /locus_tag="ACHKNB_RS10155"
                     /old_locus_tag="pgaptmp_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4195550.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNEGRVSRRLP
                     VTTSSDTGVELLPVEITPDEQKEPVVAIAPSLSTTTQTRVSASSCKVEFRHGNMTLEN
                     PSPELLTVLIRELTGRGR"
     gene            16916..17341
                     /gene="nudI"
                     /locus_tag="ACHKNB_RS10160"
                     /old_locus_tag="pgaptmp_002032"
     CDS             16916..17341
                     /gene="nudI"
                     /locus_tag="ACHKNB_RS10160"
                     /old_locus_tag="pgaptmp_002032"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HGU4195551.1"
                     /translation="MRQRVIVCPVIQNEGEFLLCKMASDRGVFPGQWALPGGGMEPGE
                     TMETALRREIKEELGKELHITDVKPWAFRDDIRTKTYPDGTHEDIYMIYLIFDCISTN
                     RTVTFNEEFQGIVWASPDTLKYMDLNEATRITFSQKGIL"
     gene            17399..17565
                     /locus_tag="ACHKNB_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /pseudo
     CDS             17399..17565
                     /locus_tag="ACHKNB_RS10165"
                     /old_locus_tag="pgaptmp_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            18023..18166
                     /locus_tag="ACHKNB_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /pseudo
     CDS             18023..18166
                     /locus_tag="ACHKNB_RS10170"
                     /old_locus_tag="pgaptmp_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            18238..18510
                     /locus_tag="ACHKNB_RS10175"
                     /old_locus_tag="pgaptmp_002035"
     CDS             18238..18510
                     /locus_tag="ACHKNB_RS10175"
                     /old_locus_tag="pgaptmp_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195552.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFIAGHSSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            18491..19697
                     /locus_tag="ACHKNB_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /pseudo
     CDS             18491..19697
                     /locus_tag="ACHKNB_RS10180"
                     /old_locus_tag="pgaptmp_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            20160..20384
                     /locus_tag="ACHKNB_RS10185"
                     /old_locus_tag="pgaptmp_002037"
     CDS             20160..20384
                     /locus_tag="ACHKNB_RS10185"
                     /old_locus_tag="pgaptmp_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4195553.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            20381..20767
                     /locus_tag="ACHKNB_RS10190"
                     /old_locus_tag="pgaptmp_002038"
     CDS             20381..20767
                     /locus_tag="ACHKNB_RS10190"
                     /old_locus_tag="pgaptmp_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4195554.1"
                     /translation="MKFVSSQEVIEFHDRLISRDGGVAGMTEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGVHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(21051..21204)
                     /locus_tag="ACHKNB_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /pseudo
     CDS             complement(21051..21204)
                     /locus_tag="ACHKNB_RS10195"
                     /old_locus_tag="pgaptmp_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(21313..21594)
                     /locus_tag="ACHKNB_RS10200"
                     /old_locus_tag="pgaptmp_002040"
     CDS             complement(21313..21594)
                     /locus_tag="ACHKNB_RS10200"
                     /old_locus_tag="pgaptmp_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4195555.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPASRL
                     HGHADRYKIKLRTSGYRLVYQVIDEKVVLLVISVGKREGNEVYQIADMR"
     gene            complement(21584..21835)
                     /locus_tag="ACHKNB_RS10205"
                     /old_locus_tag="pgaptmp_002041"
     CDS             complement(21584..21835)
                     /locus_tag="ACHKNB_RS10205"
                     /old_locus_tag="pgaptmp_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4195556.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVVILNRNEPAFYC
                     VPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
     gene            23028..23228
                     /locus_tag="ACHKNB_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /pseudo
     CDS             23028..23228
                     /locus_tag="ACHKNB_RS10210"
                     /old_locus_tag="pgaptmp_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(23275..24381)
                     /locus_tag="ACHKNB_RS10215"
                     /old_locus_tag="pgaptmp_002043"
     CDS             complement(23275..24381)
                     /locus_tag="ACHKNB_RS10215"
                     /old_locus_tag="pgaptmp_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4195557.1"
                     /translation="MNKTITALTVMMSSFAANASVLPETPVPFKSGTGAIDNNTVYIG
                     LGSAGSAWYKLDTQAKDKKWTTLAEFPGGARDQATSVFIDGNLYVFGGVGKNNEGLTQ
                     VFNDVHKYNPKNNTWVKLMSHAPMGMAGHVTFVHNGKAYITGGVNQNIFNGYFEDLNQ
                     AGKDSKTTDKINANYFNKKSEDYFFNKFLLSFDPATQQWRYAGESPWYGTAGAAAVSS
                     GDKTWLINGEVKPGLRTDSVFELDFTNTGVNWSKLPSVSSPDGVAGSFAGISHDALIF
                     AGGAGFEGSRKNYQNGKNYAHEGLKKSYSADIYLWYNGKWGKSGELPQGRAYGVSLPW
                     NDSLLIIGGETTGGKAVADSVLISVMGNKVTVQN"
     gene            complement(24401..25114)
                     /locus_tag="ACHKNB_RS10220"
                     /old_locus_tag="pgaptmp_002044"
     CDS             complement(24401..25114)
                     /locus_tag="ACHKNB_RS10220"
                     /old_locus_tag="pgaptmp_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGU4195558.1"
                     /translation="MKRYQVVSGILLCASFSVISNAATLDIRGGYRSGSHAYETRLKV
                     SEGWQNGWWASLESNTWNTIHDNKKENLALNDVQAEINYAIKLDDKWSVRPGMLTHFS
                     SNGTRYGPYIKLSWDVTKDLNLGIRYRYDWKTYRQEDLSGKMDRDNAHRWDGYLTYKI
                     NKDFTFAWQTTVYSKQNNYRYGNHKKDATENAFVLQYHMTPDITPYIEYDYLDRQGVY
                     NGKDNLSENSYRIGVSIKL"
     gene            complement(25258..25362)
                     /locus_tag="ACHKNB_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /pseudo
     CDS             complement(25258..25362)
                     /locus_tag="ACHKNB_RS10225"
                     /old_locus_tag="pgaptmp_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462246.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(25362..25747)
                     /locus_tag="ACHKNB_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /pseudo
     CDS             complement(25362..25747)
                     /locus_tag="ACHKNB_RS10230"
                     /old_locus_tag="pgaptmp_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25771..27258)
                     /locus_tag="ACHKNB_RS10235"
                     /old_locus_tag="pgaptmp_002047"
     CDS             complement(25771..27258)
                     /locus_tag="ACHKNB_RS10235"
                     /old_locus_tag="pgaptmp_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195559.1"
                     /translation="MNTLQNKTPWYKHLNRSQWRAFSAAWLGYLLDGFDFVLITLVLT
                     EIQGDFGLTTVEAASLISAAFISRWFGGLVLGAMGDRYGRRLAMVVSIILFSLGTLAC
                     GLAQGYITLFIARLVIGMGMAGEYGSSVTYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRVLFFIGILPIIFALWLRKNIPEAEDWKEKHEGKAPVRTMVDILY
                     RGEYRIANIAMTLVAAVALWFCFAGNLQNALIIAGLGVLCAVIFISFMVQSSGKRWPT
                     GVMLMIVVLFAFLYSWPIQALLPTYLKTELSYDPSTVAQVLFFSGFGAAAGCCVGGFL
                     GDWLGTRKAYVFSLLASQILIIPVFTIGASSVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGAVAPVLGAVVAQRLDLGTALCSLSFGLTFVVILLI
                     GLDMPSRMQRWLRPEALRTHDAIDGKPFSGAGVIKNESSLEMKSR"
     gene            complement(27339..28232)
                     /gene="nanA"
                     /locus_tag="ACHKNB_RS10240"
                     /old_locus_tag="pgaptmp_002048"
     CDS             complement(27339..28232)
                     /gene="nanA"
                     /locus_tag="ACHKNB_RS10240"
                     /old_locus_tag="pgaptmp_002048"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015962808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4195560.1"
                     /translation="MTADLKGIMSALLTPVDAEQKLDQDSLRRLTRFNIEQGIDGLYV
                     GGSTGEAFVQSVQERESVLEIVADEAKGKLKLIAHVGCVSTNESQSLARAASRLGYDA
                     VSAVTPFYYPFSFEEHCEHYRAIIDAADGLPMVVYNIPALSGVKLSLEQISTLVTIPG
                     VRALKQTSGDLYQMEQIRRSHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYK
                     RIAGALQNGDIKTAKYLQSECNKVIDLLIRFGVFRGLKTVLHYMGIIDAPLCRKPFSG
                     VNAEYMPELKSLAETLMKEKT"
     gene            28678..29252
                     /locus_tag="ACHKNB_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /pseudo
     CDS             28678..29252
                     /locus_tag="ACHKNB_RS10245"
                     /old_locus_tag="pgaptmp_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382922.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT         7565 a         7003 c         6891 g         7793 t
ORIGIN      
        1 agcgaaaaat ccccccgcgt cagcgccagt gcttcattaa tgcgcgcacc ggtgttccac
       61 agtgtggcca acagcatctt gcggcgcaga tccgggacgt aatggagcag ggcactcact
      121 tccggagcca gcagatattt cggcagttca tcatgaacca tcgacatctg gcgaagtgcc
      181 agagctgccg gataatcaat gacaacagga agttgtggag actgtccggc agaatacact
      241 gccggtacca tggctgcaga ctggctgtgt ataacggagc ctgacattta tattccccag
      301 aacatcaggt taatggcatt tttgatgtca ttttcccggt ggctgagatc agccacttct
      361 tccccgataa cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca
      421 tccccgacat gcaccaccgg gtaaagttca cgggagactt tatctgacag caggcgggca
      481 ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa taatgtcact ctggacatcc
      541 acaaacagac gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca
      601 gtccctgttc tcatcggcaa aagagccatt catttcaata aacctggcga cctctgccat
      661 cccttcctga ttttcagcct gccagcgttc agcacgcagt cgccgggctt cgttctgcat
      721 ggtcgtactg accagaccgg agatattcac gtcatacgcc ttgagcagct gatagctgtc
      781 gctgtcaact gtcactgtaa tacgctgctt catatcacac ctctttttaa catacttctg
      841 gtatgcatat cagtatatat tcttatgccg caaaaatcag cgcgttaata cacatactgt
      901 tatcttgctt ttagtaagcc ttatttattt taccttgcat tatgttaacc aataaaaatt
      961 aaaatcagac ttataaaaac aaagcgtaat tatcgaagga ccatttccgg cagatccgat
     1021 ttttctgtta ttgacgggtt tccggaagtg taccacgctg aggaaatcat catatcgctg
     1081 ttttccgccc ctgcgtggcg catcacaggt cagggaaata acacaacata tagtgtcatg
     1141 agcagccgaa tgaagcacta catgttgtac ttacaggagg tggcgttgtg tgggggagag
     1201 gaagatccgt gaccaggggt tttcggatct ctggagcaga aaagaaaatt attgccagat
     1261 aatcacaaaa aatggtggat tattgtgatg aagtgattat tgcaatcaga acaataactg
     1321 gaattgtaaa gatcatattc atcaataaat agcagagtca cagatctgct gaaagccaaa
     1381 ccatgaagat actacccgag tccgcttctg gaagatccat ttcaaatatc cggatacaca
     1441 ggcaactgtg gcgacgattg taatgacatt aaccatatcg gcttccctga agcattggtt
     1501 atggcaacca cgttatcaat atctttccat cattttcagt aaatatcact aacagaccga
     1561 tattaccgga cctgtaagtt atttgtgact gatatcacca atttgtaaat caaatgtgaa
     1621 aatatggttt atctgtgctt tatttttccc cgacataaaa tggaggtctt tatgaaagat
     1681 gtcagtattt tgaatgaaat gatcttcagc gcatcaacag aaaatgaact acaggaaaaa
     1741 tttaaagata tatatcaccg tatcaacaac gagagtgaat atgatgattc tggttcagtc
     1801 gaatttctgc cacttccaag actgaattcc cgttctgaac gctcagaagt acaggacatc
     1861 atttcagttg taattgaaag cggatttttt accagtggcc cctatattgg aaaagtagaa
     1921 gagacattaa gattatttta tggtgctcat acctgtattg ccaccagtag cggcaccgat
     1981 gcgttaaaaa tagctcttaa atctgttgga gtggaacccg gagatgaggt catcgttccg
     2041 ctgaactcct ttgcagctac ggagaatgca gtgatggccg tgggagccgt tccggtcttc
     2101 gctaatattg acagttcttt caatatgctt ccggatgaag tggatcgcct caggacttca
     2161 aagacaaaag ccgtgttgcc ggtctgcctt tatggctcat gcatgtacat tgatgccatt
     2221 caccggattg cccgcaaagc cgacattcca gttatcgtgg atgcagcaca gtgctttggt
     2281 atcaggtcac tcatcaacca ctgtgatctg ctggcgttga gctttaatcc attcaagaat
     2341 attggttctc ttggtaaatc cggtgcagtt ctaacccttt ctcctgaact ggcacgcctg
     2401 gcaaggcaat attcatatca cggatttgct gaaggtaaaa agaatattaa ggcccagaac
     2461 tggggattaa acagccgtat ggataatctg caggctgcca cgcttagtgt caaacttcac
     2521 cattttgaaa ataatgcaaa aaaacgctgc cttcttgcag cccgttacca tcttttactg
     2581 tctgacctgt cacataacat aatacttccg acagagaccc atcagaatac atggcacctt
     2641 ttccctattc tgctctgcaa aggcgaacgt gattctctgt tcgcctttgc tcgtgaaaag
     2701 ggagtggagt tagatattta ctatcccgtc ctttcacatt gcggagagca tccattggca
     2761 accggatatt cccgtcgaga gcagttcagt gattctgaaa aaatacattc tgcattactt
     2821 catctgccgc tgcataacca tatgtcgctc caggagcaaa acatcgttat cgaggtactg
     2881 catgactatt ttaaataaag actggcccgt cgaattcccg ttcccgacag taatccgcac
     2941 tgtggtgtgc agcgatcttg atgaaactta tattccttcg gacagcgata aaaaaaactg
     3001 agtgggatcg ttcagctgga atcctatatt acatcccatg ccgaaaaaaa ggggattctc
     3061 gctggctgga tcacaggaac aaatctggat tcagcctggc gaaaatccag gggatatata
     3121 tcgcgaagcc ctcactttat atgttgcagt ctcggcaccg aattttactg ggtgaaaaat
     3181 ggtcgtctca tcccctctga aacctgggca gaaagaataa gccgttcagg ctacagtcgt
     3241 cagaatgtca actgtctggt taagattctg atggaaaaag agatccccct tctgaaacaa
     3301 ccagaggatt atcagggccc ctttaaagtg agctattact accctgagag cccatctatt
     3361 gccagcgatt ttgccaccat ccaggaactg gctgacgagc gacgaattcg tgttgtgttt
     3421 acccgctgta acccggcggc tggcgatccc tctggctgtt atgacgttga atttatcccc
     3481 ctctgttgtg gcaaggatca ggccgtgtct tttctgatgg aggaaaccgg gctgccgaaa
     3541 gaagccatta ttgcctttgg tgacagtgcg aatgattttg ccatgtttgc acaggccgga
     3601 agtggctatc tggtgggtaa tgccgacccg tttgcagtca gccagtacgg aaacagactg
     3661 aacagtgctt actgccacgg gatactgagc gttcttaagg agctacagtg atgattatcg
     3721 gaattgtcgg ctgtgggggg attgcccgtg cccatgtcaa cgcactttgt ctcaatccgc
     3781 tagtaactgg tctggccctg tacgatgtga atccagacaa agtccgacag ctcagcgaaa
     3841 taagctccct gccggtaaca aactgccagg atctggagca gcttgccgga gtcagtcaag
     3901 ggttcattat ctgcacacca aaccatctcc acgttagcgt ggcagaagaa gtgcttcgct
     3961 acaaacagct cccgtttgtg tgtgaaaaac ccctctcaac agatctcaac tctgcacgat
     4021 atctacagga gattgctcca aaaggtagta tcgtgtcttt taactaccga tataaccccg
     4081 tcatcgacat gataatgcgt ctgagaaatg agaggaaatt aggtgaactg catttcttct
     4141 cagcagaatt taataaaaat tctgcactga cccgccatca cctgacctgg cgagactccg
     4201 cacaacagag caaaagcagc ggtgctctgg gcgatctatc ctgtcatctg cttgatttat
     4261 tttgttttat aggggaaagc ccggtagtgg ttcatggcat caaaacggta aaggggacac
     4321 gggtgggtac aaaatcagat ggtcaggttg aagtggatga taacggctat gtaatgggca
     4381 gttcagaaaa gggagcttat ttcagagttc atgccagtaa atcggaaaca gatcagaatt
     4441 tgggactgca tatacagctt gtatttgaaa atggtgaaat cagatattca acccaccatg
     4501 aaaatcgcct gctcctgatt ctatttaatg atacgaatac ggaaacaatt ggcttcgacg
     4561 ctctgaaacg cttgcctgat ccaccacggg agcttccatt ctggtctgat tcgtttattc
     4621 atcttcatga cgactggtgt gctcttatta aatatggtca ctcgtctcct aagctggcgg
     4681 acctgtcatc cggccttcat atacaggaaa taattgaggc attctgatat ggatacaatt
     4741 cttttaaccg ggctgtttgc tgcatttttc acaacgtttg cgttcgctcc ccagagcata
     4801 aaaactatca ggacacgcaa taccgaaggt atctccgttg ttatgtatat catgttcctg
     4861 acaggtgtaa tctcatggat agcgtatggc attatgcgat ctgattttgc cgtactgatc
     4921 gccaatattg tcacactgtt tctggctgca ccagtgcttg taataacact tatcaaccgc
     4981 aggaaaaaac acgtatgaat attgcaatca tcggcgcggg tcctgccggc atcatttctg
     5041 cccgtaatgc gattaaagca ggtcactccg tggttctgtt tgagaagaat acccgaattg
     5101 gggggatctg gaccccctgg agtggtggcg cttaccgtaa tgcctgtatg cagaactccc
     5161 gttatacatt tcattatact ggctttcccc ctggcgatat cgatgaattt ccaggagtgg
     5221 aacaggtatt cagatatctt tcggccgtag ccggagagga tgccctccgt gagtcgaccc
     5281 gactgaatac tgaagttgtt tcactcagaa aagacgccgg acactgggtg atccgctgcg
     5341 cttctgaagg aaaagacacg gaagacattt ttgaccgggt catcattgca acaggtgaac
     5401 tctggcaacc ccgccggccc cccctgccag gtgaggaaaa cttctccgga acgttgatca
     5461 cgtcgagaga ttatcaggag ccagaggcat ttaaaggaaa aaatatcctc atcattggcg
     5521 gcggtgtcag cggtgcggat attgcctcag accttgttcc ctttgccaga agcgtaagtc
     5581 tgtccgtcaa aaagatggga ctttatctgc cgagacaatt cccgactggc ccgaatgaca
     5641 tgatgcactc ctatctgggc aggtgtctgc tgagccaaat gaattatgaa gattttatcg
     5701 gttatctcga caccatgatg cctgactaca tgcaggccta ccgagcatcc ggtctgttgc
     5761 ccgacatggc gaacaataac gcggtacatg ttaacgaaaa aatcataccg aatgtagccg
     5821 ccggtctgat aaaagttaaa cccctggcag agcgtttcac cggagaaggc gctattgagt
     5881 tcgtcgacgc atctcaggaa aaatatgatg taatcataac ctgtacaggc tatgaaatgc
     5941 ctgattactc gttcattcag ggatttgaca gaacacagct ctatgagcac tttttctgga
     6001 cagaggaccc ttctctggcc gtcattaatc ccccggtgga taccgccggt ttcggggctg
     6061 cgtttcccta ttttgatatc atcagccagt gggtaatgaa cgtattctcc gggaagactt
     6121 cccttccgga aaaagaagct atgcgcaaat ggtgtgctga gcacatggct tcacttcatg
     6181 ttaagcgatt ttatgacagc tggctggaaa ccatccggat tgggctgctg tcaggtcttc
     6241 tcccggaccc ggcccgcgac ttttcccgat actggaacat catcagcagt atggtcaaac
     6301 ctgcctatct ggccactcct cccgcatttc cggagcatgg tatgatggac agcctgtttg
     6361 atttccggat cgccagaata cgcatactgt cagggctggg aaacgatgct ctcggttatt
     6421 tactgaaaaa aggtgatatc actgacgcag aataccgggc ggccctggaa atcgatcccc
     6481 ggcagtcgat ttctgtacac ctgccgtatt cacagaccta cctctgataa aaagggccag
     6541 taacggccct tttctttttt cataatattc tgatcagtct tgccgggttt cctgcataaa
     6601 tgcctttgtt ctcaaccggt tttactacaa ctgcccccgc accgataaca gctccgctgc
     6661 atatatacgg agacagaata atggctccac tgccaacagt aaccgaatcc cccaggataa
     6721 tgctgatcca gttatccggt gacggatctg gtgctcctga acgaaacaga tcgttggcga
     6781 acgtcacgtt atgaccaatg aagcagttct cccccagtgt tacgttctca caaataaacg
     6841 tgtgcgactg tatccgactt ccactaccaa tgacacagcc tttctgtatc tcaacgaagg
     6901 ggccgacaaa cacattgtcc ctgagttcac attcataaat gttgaccggt ttgacaatac
     6961 ggacattggt cccataaatc acatcccgga cagcacattc cataaatttc atactcacac
     7021 gtcctttaaa tacagatgac acttgccgga attatacagc ccagattcag ttgccatgtt
     7081 tcagaatagc ttataagccg cacttttacc gatactcagc aacaaatcag gtctggatcc
     7141 acttcagccg gaaaccggat ctgcgcggca gaaaagacgc aaatgatatg ataattaaga
     7201 aatatacata aaatcatagc agatatgatt ttcatcatta aaaatgaaac aatatctgat
     7261 attatcagaa atcaaaagca caaaaatcat acatgatatt atttttacct gtcagagcat
     7321 tatcagatgg agcgtgcaga atggatcagg aaatgacgtt ttcactcagc tatgaacaac
     7381 tgacccggtt tgcggaacga cgcatccggg aatgcaatct ggacagccag ggcgcgattt
     7441 atctctgcga gtcagccaaa gccggagccg tgctgatctt ctggcatgaa ctggccataa
     7501 atggctatgc ctcaatgaat gccataaaga gacaggagct cattgacgcc gactttcagc
     7561 gcctcagaaa gctcatctgg ccggaggatg acaggtaatg acccagagcc gccgaccgtc
     7621 gccactgcag cggagagtgc tgattgtgct ggctgcgctt gatgaaaaac ggccgggccc
     7681 cgtgctgacg cgggacatag aacgggtgct ggagcgaagc ggggaagccc cggtctacgg
     7741 gccaaacctg cgcgcctcct gccgtcgtct ggaggaggcc ggctggctgc gtaccctgcg
     7801 cgccccaaac ctgcagctgg cagtagagct gacggacgcc ggcagggccg tggcacagcc
     7861 tctgctgctg gcagaacagg accgcctccg ggcagaacgg cgtgcggcag aagtcgtcgt
     7921 actgccgctg gtcccggcaa caggtctgcc cgcagacggc acttctgcca ctgatctggc
     7981 ggtggagctg aacggcatga cgtatcaggc ctgccgtggg gattttgtgg tacgtctcga
     8041 cggcagcacc tgtctgcagt tatggaataa agaaggcaag gtggtacgcc gggagggcga
     8101 tccgctggaa gtggcgcagt ggctgcaggc ctgtcacgat gcaggaatgg aggttcgtgt
     8161 gcaggttaat gaaagcatca ccccgtaaaa acatgcctgc acggtttaca aatcatccca
     8221 tggtggcagc cggttaactt tcctgcgcca ccagtagata aaccacattg cacatcccca
     8281 gaaaatgccc tataagctcc ccatcaacag cgtgacctgc cagaacggca taaagggtaa
     8341 tggcagaagc cggataccca gccgccagag aatacgcaga agaggagggg cataattact
     8401 ccgccacatc tttttgctgt ccatcagggc aatggcccgg gcttttttct actcaaaggt
     8461 cacaggagca cctccgttcc atgatgtaaa cgtgacacag cttattgtta ttgattatta
     8521 ttatcatgct gtgattatgt tatcacgcac cggtacacga taacaataaa ggagaaacca
     8581 gatgaaactg gatatatctg taaagtattt tctgaatatt atgatagcag atttcatcat
     8641 tcccgcacca gaaaaaatat caaccacatg gcactcagat actaaaatga atcgctttgc
     8701 ttgccctata cattctacta atagtgatta ctattcaggt ttttatcata cagatgtatt
     8761 aattcccgga gaactggctt aggacgccat cataatccat gatgttgata gtaatgatcc
     8821 tatgattttt aactggaatg tattgtttta tgtctcactg gaaaagtttc ctgttggcaa
     8881 tccttattat ggggaaattc caagtattcc tgtggatgaa gaagcaaagt tttttaatcc
     8941 atttccacca agagcgtttg aaaataaacc tctttatact gaggagataa ctaaatatga
     9001 ttaagggaat tatgtttgca tttcttgctg ctttttcatg gggagcagca atagtgatga
     9061 gcaaaaaagg tctcgaaaac atggatgccg gagagttgtt tttctggcaa gttggtagtg
     9121 ctgcactgtt atcatggttt gttctcgcaa ttagcagaaa aaaactcccg gtaacaaaaa
     9181 aatcaacact tgcctattca acaggaattt ttgagccatt tctggcgtat acattcactc
     9241 tctatggcct aaaatttatt tctgctggta taacatccgt tattttttca cttgagtcag
     9301 tatttattct tatactatct gttatattct tttccgttaa aattaactct ctattacact
     9361 ttatattatt gcttactgga gcaatggcag gcagtataat ggttgcccta cctgatatca
     9421 atgaccgcat tgataatatt acaggatata tacttattgt tgcaggtgta ctctcagcat
     9481 ccttttatgt tgttatctca tcacgactta ttgtgaattt tgagcctgtt acgttgttaa
     9541 ctgggcagtt gactttctct tttattcttg cttgtttttt tattgttcta actgacacat
     9601 ccctgcaact gccaatagaa tctgtatttc ttgtgttttt gtcaggaatt ttacaatatt
     9661 ttttggcttt ttgtttctat ctttattccc tacgctggat tcccgttcac atagcaggag
     9721 caatgctcta ttttattcct ctggtagcat tgctgttgtc ctggttcttc ctgcaggaag
     9781 atatatcatt tattcaggga ctgggcgttt ttataactat tttatgcgta tatattctca
     9841 ataagaaata cgccatcgaa tgactttatt agggtcgcgt tatcgcggta gggtacaccc
     9901 tgacaccata accaaaaacg tgatgccaga cgatgaatga cataacctta atgcgataaa
     9961 gtggtaaaaa atgttcatca gagctgattt aaaagaagta aagcaatagc ccggaccaga
    10021 gacaaggcat catgcaacaa atgtaacgga tattttgaaa tccttgcctc cgcacatgac
    10081 tgggcataat taaagaaaag cgtatgctga cacagctgca cagagtgctg taccgttgtc
    10141 caggacacta tgtttatggt tcagccacac agtctaccgg cacaggtact ggctctctgg
    10201 cctcccgtaa taccagtcag tcgcctttac cggcgcagca ggcaggattt ccgcgcccgg
    10261 aactgtattc actttcagtg tgccagtgac agccccaata aaaggccaga ctcaaccccg
    10321 tcaaatatgc ttattaagga gtgatgatga ccctttacca gataaagccc tcgtttcagg
    10381 cgctgttaag accgctgatg ttctggctga acaggcgcgg cgtcaccgct aatcacatta
    10441 cactgtgcgc gatggtgctt tcccttatca ccggcgtggt gctcattatt catcctcagc
    10501 ccggattatt cgtccttctg cccgtggtgt tgtttgtacg aatggcgctc aatgcgctcg
    10561 atggtatgct ggcgcgtgaa tgtaatcaaa gatcgcgtct gggggccatc ctcaatgaga
    10621 cgggtgatgt aatttccgac atctcgcttt atttgccctt tatgctactc ccgcagagca
    10681 gtataccgct ggtgctgatc gtggtgtttt gtgcgctgat gaccgagttt tgcggcgttc
    10741 tggcgcaaac gattaacggc gtgcgcagct atgccggtcc gctgggcaaa agcgacagag
    10801 cgctggtgtt tggtgcatgg gggctgtcac tgtcgatctg gccgcagatt gtcgcctgga
    10861 ataatgtcat atggagtgtc gcaacactgt tgttgttatg gacgattttt aatcgctgtc
    10921 gcagcgcatt gcgtgaagag ggtgtgaaat gacgctactt gaaaaatcac tggtcgttat
    10981 tttttccctg ctgttgattg ccaccgtaat caacgggttg ctggtgtggc tgcgtccagg
    11041 taaagactgg cgcgagctga cattacgtat tcgcacctgg tgggtgatta tcgtcctgtt
    11101 ttccttcgca ctattaagtc ctagctggct ggcactgacg ttcttcgcgc tggtcagctt
    11161 tatggcgcta aaagagtttt tgacgctggt gccttcccgc cagtccgacc gcatgccgct
    11221 gttatggatg tttattgcaa ttccccttaa ctactggctg ataggtatcg gctggtacgg
    11281 catgtttgtg gtgtttatcc cggtctacgt gttccttttt ttaccggcgc gaatggtgat
    11341 tgctggggat acacagggct ttttacgtac cgtttcccag ttgcactgga gtctgatgac
    11401 caccgtattt gccttcagcc atgtcgcgtt tttactggtg ttaccggcgg atggaatgca
    11461 aaccagcgcg ctgttggtgc tgtttttggt cggcctgacc gaatttaacg acatcgcgca
    11521 atacctgtgg ggcaagtcgc ttggtcgtat aaaagtcata ccgaaggtca gcccaaacaa
    11581 aaccctggag ggattgctgg gtggggtggc aacgacggcg ctggcggcaa cagtcctggg
    11641 gccactgttg actcccctga gctggccgat ggcactgtta gccggggtta ttatcggcgt
    11701 tactggcttt tgcggcgacg tggtgatgtc ggcaatcaag cgtgatatcg gcgtcaaaga
    11761 cagtggtacg ctgctacccg gccatggagg catccttgat cggctggatt cgctgatttt
    11821 taccgcaccg gtcttcttcc attttgttcg ctatttctgc tactaaccta tgatgacacg
    11881 actactccgt accctgttta cgctgtgtat cgtctggccg gtcatctggc tgtggcttgg
    11941 tctgcgtatc cgccaccgcg agcgactgcc gaagcacggg ccttcgataa tcgtggcaaa
    12001 tcacaacagc catatggatg ttttcgcctt gctttcgctt tttccgctgc gttaccaggc
    12061 ttctgttcat ccggttgctg ccgcggatta ttttctgcgg gataaacgaa tggcttggtt
    12121 tgcgctgaag atccttaaca tcattcctat ttcaagaaag ggcggttata ccgatccgct
    12181 ggcgcaatgt gaacaggcgc tgcgtgaagg taaaacactg atcctgtttc cggaagggac
    12241 gcgcggtgag ccaggcgtac ttgcgccatt gaaatccgga gtgtggcatt tgagtcagcg
    12301 catgccggag gtgcccatta tccccgttgg gttgcgcggt actgagcatg tgatggcgaa
    12361 aggtaatcgt atccctttgc cgctgtttat tgatgtaaat attggcgatg cgctaagtac
    12421 gcatccggag aagaaaacgt ttatggacga cctggagcaa agactgacgg cgcttttgca
    12481 accctcgcaa ggaaatcatc cccatgacta attcccgcat acccgatgaa cggcagtttc
    12541 tgaccagtga taatacagag ctgttttatc gccactggcc agcacaagaa gccagttcaa
    12601 cgccaaaagt ggtggtcctg tttcatcgtg ggcatgagca ctccgggcgt ttgcaacaca
    12661 ttgttgatga gctggcgatg ccggatacag cgttttatgc catggatgcc cgaggtcatg
    12721 gtcgctcacc gggtctgcgt ggttacagtc cgtcgctcgt gcgttcggtg caggatgttg
    12781 acgagtttgt gcgtttcgcc gccgcagata gccaagctgg gatgacggat gtggtcgtcg
    12841 tggcgcaaag cgtcggggcg gttctggcgg caacctgggc gcatgattat gccccggaca
    12901 ttcgtggcct ggtgttggca tcaccggcgt ttaaggtcaa attgtatgtg ccgtttgctc
    12961 gccaggggct ggaattgatg tatcgcctgc gcggtctttt cttcgtcaac tcgtatgtga
    13021 aaggcaagta tctgacgcac gaccccgagc gggtagcgag cttcaataag gatccgctga
    13081 ttacccgacc aattgctgtg aatattctgc ttgatctcta caaaacggct gagcgagtcg
    13141 tcagcgatgc cagcgccatt acgctacctg tgcagttgct ggtctccggg gaagattacg
    13201 ttgtgcaccg ccagccgcag cttgattttt atcagaacct gcgaaatccg cagaaagaac
    13261 tgcatgtact gccgggattt tatcatgaca ccttaggcga aaaagagcgg catctggcat
    13321 ttgacaggat gcgcagtttt attgatacct tgtatgcccg tcccgtgcag cgttttgatt
    13381 acagccagga agatcgctgg agccccggcg ccgatacctg gcgcatactg agcggtggcc
    13441 ctgcgctgta ttcgctggac gatatctctt accgctgttt gcgctacggg atgaaagtgc
    13501 tgggcacgca gtccacgggg gtgcgtctgg gatttgagac tggtttcgat tccggcagta
    13561 ccctagacta catctatcgc aaccagcctc agggcagcac ctcgttgggg cgcctcattg
    13621 ataaaaacta cctgaatagc gtgggctgga aggggatccg ccagcgtaaa attcatctgc
    13681 agatgttaat tcagcaggct gttgctcggt taacagagca gggtagcgcg gtccggatcg
    13741 tggatattgc cgccgggcat ggacgctatg tgcttgatgc cctgcaaggc gaaacggcgg
    13801 taagcgagat ccttctgcgc gattacagcg aattaaacgt ggcgaaaggt caggagatga
    13861 tagcctctcg cggtatggcg ggccgtgcgc gatttgagca gggggatgcc tttaatcgta
    13921 atgaactggc ggcactggaa ccgcgtccga cgctggggat cgtgtccggt ctgtatgaac
    13981 tgttcccgga aaacgggccg gtacgagatt cgcttgccgg gctggctgca gcgattgaac
    14041 cgggaggcat tctgatctac acctgtcagc cctggcaccc tcagttaaaa accatcgcct
    14101 ggtcactcac cagtcacaaa aatggcaaag cctgggtaat gcgcgtacgt actcagggcg
    14161 agatggacgc gctggtccgt gacgccggtt ttgagaaatg cacgcaacta attgatgaaa
    14221 tgggcatctt cacggtctct atggcagtac gacgtcaacc atgacaacgc ggcgtgcggt
    14281 gattattcag ggaatcggct ggctaatggt gctggccccg ttcttctttc ttacttatgg
    14341 catgacgaat cagtttaccg ccgggagaga aaacatcagc agcctggtgt ttgactggga
    14401 gcaccggatc ccgttaatac cgctgacgat tattccgtac tggaggtaag cgtcaacgga
    14461 gcaccgtatt gacgcttatt tattggtgag tactacgttc catggcagga gttcatcaac
    14521 tcggttggaa ggccattccg gcagtacgct caggatatgg cgcagatacg cttccggatc
    14581 gataccgttc agacggcagg tgccgatcag cccgtacaac agtgctccac gctcgccgcc
    14641 gtgatcgctg ccaaagaaca taaagttttt ctttccgaga cagactgcac gaagcgctct
    14701 ttccgcagca ttattatccg cctctgccag accgtcatca ctgtaataac agagggcgtc
    14761 ccactgattc agtacatagc tgaacgcttc gcccagtctg gattttttcg acagcgtgcc
    14821 attcttctcc accatccatt catgcagcga cgtcagtaac gatttgcttc gctgctgcct
    14881 gcaagacgct ctgactctgg taatccccgt atttcatcct cgatggcgta cagttcactg
    14941 attcgcttca gggcttcttc tgccgtcgca cttttgctgc tgatgtatac atcgtgaatt
    15001 tttctccggg catgggccca gcacgcaact tctgtcagcg caccaccttc acgttctgca
    15061 ctgaacagcc tgtcgtaacc tgtgaacgca tccgcctgca ggataccccg gaaggggcgg
    15121 aggtgctgct ccgggtgttt cccctgccgg ttcggtgagt acgcgaacca gaccgccgga
    15181 ggagatgacg aacccgcatt gcgatcatcc cggacatacg tccagatgcg ccctgttttc
    15241 gtctttttct ggcccggtgc cagtaccttt accggtgtgt cgtcagtgtg aagcttgcgg
    15301 gtattcatca cataacggta cagggcatca ttcagcggcg tcattaactg gcagcacgcg
    15361 tcaacccagt tggagagtaa tgcacggctc agttcgacac cctgtcaggc aaagatttca
    15421 ctctgacgat acagtagcag gtgttcgcag tattttcccg ttaacacgcg ggcaagtaat
    15481 ccggggcccg caataccacg ctctatcggg cgggacggtg ccggtgcttc aacgatgcag
    15541 tcacattttg tacaggcttt ttttacccgt tctgtgcgga tcactttcag ggcgctgctc
    15601 accagttcca gttgttctgc actgacctcc cccagataat ccagctcacc gccacactcc
    15661 gggcaacagc tttcttcaga ctccagacgg tgtatttcac ggggaaggtg tgccggtaac
    15721 ggacgacgat ggcacgactg tcgcaactgg cgcagttttt ctatctgagc ccgcagatgt
    15781 tctgcttccc gttcatcttc ttcgatcttt tcttcggcac gtgtcagtgc agagcgcagg
    15841 aaggcttccg tctcttcaac cagactcagt tgctggtctt tctgacggag ggcttcagcc
    15901 tgctcagaga gcaacctttc cagctctgcg atgcgaatga ggtatttctg actcatgacc
    15961 gtttttataa tgcggtcagg agttttttac aacattgtca gtgagttacg gcgggatgtt
    16021 tttggctgac gccagtccag cttatcgagg agcattgcca gttgcgagcg ggtaatggat
    16081 accttgccgt cacgtaccgc aggccagata aactggcctt cctccaggcg tttggtgaac
    16141 aggcacagac catcagcatc agcccaaaga attttaacgg tgtcaccccg tcggccacgg
    16201 aagataaaca ggtgaccgga gaagggatta tcattcagca catgttgtac ctgttctccc
    16261 agtccgttga aggatttacg catatcggta acgccggcaa cgagccagat acgggtacct
    16321 gatgggagtg agatcatctt cccctcccgg tcagttcacg gatcaacacc gtgagcagct
    16381 ctggcgatgg attttccagc gtcatgttac cgtgacggaa ttccaccttg caggagctgg
    16441 cactgactct ggtctgagtg gtagtggata aagatggcgc aatggccact acaggttctt
    16501 tctgctcatc cggcgttatt tctacaggta ataattcaac gccagtgtca gaagaggtcg
    16561 ttaccggaag acgccgcgaa acacgccctt cgttctgcca gagcctgagc catttgaaca
    16621 ggaggttatc attgatatcg tgttcccggg cgatacgggc aacagaggct cctggttgca
    16681 aagccagttt aaccatttga agtttaaaat tatttgaaaa tgatctgcgg ggttctgcgg
    16741 ataatgtttt ttgctccata acaggcgtcc acaggatggc atgcggaata tcaacatttt
    16801 actgataatc ttccgttaca ccttcgtgag cggattatgg tatgtgctca cccggatgac
    16861 attgatgatt tttctgataa ggttatccaa aaagccatga tgttgaggac ataaaatgcg
    16921 ccagagagta atagtgtgcc ctgtgataca aaatgaaggt gagtttctac tttgtaaaat
    16981 ggcctctgat cgtggagtat tcccgggcca atgggcactg cccggtggag gaatggaacc
    17041 cggagagact atggaaacag cattgaggcg tgaaataaag gaagaactgg gaaaagaact
    17101 gcatatcact gacgtaaaac cctgggcctt tcgtgatgat atccgtacaa aaacgtaccc
    17161 ggatggtacc cacgaggaca tttacatgat ttacctcata ttcgactgta tcagtacaaa
    17221 ccgtacagtt acatttaatg aggagtttca gggtatagtc tgggcatctc ctgacaccct
    17281 aaaatatatg gatttaaatg aagcaacccg gatcacattt tctcagaaag gtatccttta
    17341 aactccaatg ctaatcgtgc aactgataac ggcttccaca acagggagcc gttttcttat
    17401 gccactactc aaggatctgc tcgatttcag tgaccatccg cttatgcctc cacactctgc
    17461 acaactgttt gctgaacacc ttcccgtcga gtggatacag cactgcctga cgcgtcctgc
    17521 tcatgagacc gttcggttcg ccgtcgtcgt ttaccggggg acatgggatc cgcttggaat
    17581 tcggaaattt ccccacgcta agagaattga gttgaacaag tgcttacatc ccggcaagct
    17641 aactcactga atgtcaaggc tatacgaaat gttctgaccg tctgataacg accgggcccc
    17701 gtgctgacac gggacattga acgggtgcta gagcgaagcg gggaagctcc ggtctacgga
    17761 ccaaaatgaa aaacagggct tctggagaaa atggttccac taaatagctg cgccgaatag
    17821 tagatcactt tgagggaact cagcccggat tgcgcgatct gatcaattgc caaaccaatc
    17881 aaaatcacca accggactga gcgatgccga tcatagcacc aattccccgt gacgaactgt
    17941 cacgaacggt gcaatagtga tccacaccca acgcctgaaa tcagacctag gggtaatctg
    18001 ctctcctgat tcaggagagt ttatggtcac ttttgaaaca gttatggaaa ttaaaatcct
    18061 gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac
    18121 ggttaaacgt tatttgcagg caaaatctga gccgccagaa ccacggcagc ctggcggctg
    18181 caaatcgggg tgtggcagaa tatgagcttt cagagtaacc atccagaatg agttcccatg
    18241 tcacacaacc tggagcacca gaaagtccac accagaatgg taaaagaggt tctgaaagcc
    18301 gtcgccagag caaataatca cccttatcag tccgtatttg cagactttat cgccggtcat
    18361 tcctcctgca cggtctgttt ctgggaaacc ttccacaaaa tgtacccgga ttcaccgtac
    18421 gaatacgtca cgttctgcca tacctgccgt cgttttgatt tatacgaaac agaagcggag
    18481 atgaaggcgg atgacccgaa gtggtggtga ttttcagccc cgaccgctga aacgcctgtt
    18541 cacagccaac cagtgctgga cgtccttcct ggatgcgggc ggtctgcgcg atatcgaagt
    18601 ggaagccgtc accaaaatgc tggcctgcga cacgcggata ccgggcgtga aggaatacaa
    18661 ctgcgataaa cctgactgcc cccacgtcag atacgtcact aactcatgcg gctgccgtgc
    18721 ctgcccgtcc tgcggaaaga aggccacaga cctgtggata gcgacacagc tgaatcgtct
    18781 tcctgactgc gactgggtac acctggtctt caccctgccg gacacgctgt gccggtgttc
    18841 gaaagcaacc gttggctgct gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat
    18901 gccgcccgaa aacgggggct ggaaaccggt atcttctgcg ccatccacac gtatggccgt
    18961 cgtctcaact ggcatccgca tgtacatatg tctgtaacct gtggaggtct gaataagcat
    19021 ggtcagtgga aaaagctgag cttcctgaaa gacgcgatgc gttcgcggtg aatgcggaat
    19081 atgcggcagc tgcttctgaa agcgtggtct gaggggctgg cgatgccgga gtcgttgtca
    19141 catatcacga cggaatcaca gtggagaagc ctagtgctga aagccggagg aaaatactgg
    19201 catgtgtaca tgtcgaagaa aacggccggc gggaggaata cggcgcgcta cctgggtcgt
    19261 tatctgaaga agccgccgat agcagcctcc cggctggcac attacaacgg aggggcgagc
    19321 ctgagcttcc gttacctgga ccacaaaacg ggagaaacgg cgacggaaac gctgacacaa
    19381 catgagctgg tcgcgaggct gaaacagcat atcccggaga agttttttaa gatggtgagg
    19441 tacttcgggt tccttgtcaa ccgggtgtgt ggagagaagc tgccgcaggt gtaccgtgca
    19501 ctggggatgg ataaaccgga agcagtggcg aaagtgtgct atgaacaaat ggtgaaacag
    19561 ttcctgagtc gtgacccgtt cgaatgcgtg ctgtgtgcgg ccggatggta taccgccagg
    19621 cccttgcggg gctgaatgta tcagggctga agaaaaacgc gcgggatatc agtctgctga
    19681 ggtatatgcc ggcctgacgc aggctaagtg tgcccgggaa ttggtgcagg aataaaaaac
    19741 agcctgtcgc ggtataaaaa ccgcccatac acagagagaa aacaccggag tggagggaaa
    19801 agcagaagag ctcacgtggc ataaggcgtg gacagcggta tcgtctgatt tttcaatttt
    19861 gtttttcaat ttcctatacg tgaacttcac ttttccttag cgtgtattat cgcagaaatg
    19921 cccacggaac ccttacaata ttgtacgttt cccaaacgca gcccatcagg cgcggaactc
    19981 atcgaggcgc gggaataaaa tcccattcag acgcaggata gattcaagca agctcactta
    20041 tcgtcaaaat cggtgttgca aaaacgggag tgaccataga ttcgtttccc aaaccgcccc
    20101 cttttatatt gaccagaagt gttgtacagt ttaattgtac aactcaaaga ggaagcacta
    20161 tgtttacatg taactatagc catgctcgcg ataacctggc ctcgttgctc gagctggcgg
    20221 ttgatgaaaa tcagcctgtt gagattcagc gccgtaaagg tggcgcggct gtactagttg
    20281 ataaagacgc ttttgaacga taccgtaaag cggctcttga tgctgagttt gctgagatca
    20341 tgcaaagtca tgggacggcc tatacggagc tttctaagcg atgaaattcg ttagttcaca
    20401 ggaagtgatt gagtttcatg accgcttgat ttcgcgtgat ggtggcgtgg cgggtatgac
    20461 agaacccggt cgcgctgacg cgatcattca tcgtgttttg aacatgtatc attatgaagg
    20521 tgtgacagat attatggatc tggctgccgt atatttagtt gctattgctc gtggtcacat
    20581 tttcaacgat gcaaataagc gaacagcctt gtttgttgca caggtttttt taaaacggaa
    20641 tggtgtacat attattagtt cacgtatttc gtttgatgaa atgcaaatta tcgctttgaa
    20701 tgctgcaaca ggcgaatata actggaagat ggtttctgac catcttaaag ccattattct
    20761 aaattagata tagatatcca atcagcagca ttttacaatc tcctgagcaa tgtaaaaatc
    20821 atatatcttt ttactatata atttctggcg tatgtgctca attcagacct tacacccttc
    20881 cgcttcctcg cccactcgct cactaccgtt cggtcgctgg ggctgcggcg agcgtattgt
    20941 aagtttttca atacagacga cgttctccct ggctgattaa tattgtgtaa taatacatat
    21001 cgttttcagg aataatgcgg tcaattactc tgatgggcaa ggccagatta cctggcagct
    21061 tcggaaattc cccctagtgc ctcgattacc tggtgtaacg caacaaaaaa aaccgcctaa
    21121 accaccaggc tcatttattt cttccagggc attagcaaga taagcgtttg cataatcagg
    21181 atctgcgcgc aattcttcaa tcatggcgac gttgtgatca acggttgtcg aatagcatga
    21241 aagattcaag tgagcctcca gagaactaat tcagggcttt caaaaaagaa tccttgcttg
    21301 attatcagga tttcagcgca tatctgcgat ctgataaact tcgttacctt ccctttttcc
    21361 aacggaaata acgagtaaaa caactttttc atcaatgact tgatatacaa gtctgtaacc
    21421 agatgtacga agtttgattt tgtaacggtc agcatgacca tgtaacctgg atgcaggcac
    21481 gcgtggattt tccaggcgtt cagacagttt ctttttgaat tgttcgcgga tggtgtggcc
    21541 cagcttctgc cattccttca gtgccctgcg gtcaaaagcc agttcatagg tcatccaggt
    21601 taaccttcac aatttccgga tttttcatgc gctcatcagc aacagcgttt aattcggcat
    21661 cttcagcgag ttcccgataa taggcgtaaa gttttggggg gacgcagtag aacgctggtt
    21721 cgtttcggtt caggatcaca actgcgtctc cctcgccttc agctacggtt cccataggat
    21781 tttttttcag gtcagtaata ctggctgctg tagttgtcag aatttgatag ctcataagcc
    21841 ctccgtttgt ctcgttgaga taatagcatc tttaaaaaca ctcttaaaag acctttattt
    21901 tgaagggggg atgaaactga tggaataggg ggtccaggcc gcaaccgccg aaatttccgg
    21961 gatgcacaaa ttttatgttc agcaacctgt acgtcgaatt atctccttaa cgaacgtttt
    22021 cgtacggttg gccctcaacc tcaatttcaa gcagagccct ttttgatgcc gcgccagcgg
    22081 catggaaggc ggcactctgt ggttacctgt ggcacaggtg taaaaccgcc gaagcccggc
    22141 gtaagccggt acgtattgaa aggttttgcc tatcccattc ccatcctgct aagccatacc
    22201 cgctttcagt catgatgaag taagagtgcg cggtcggagt ggtcccgacg agggtttacc
    22261 cgaagtcggg gcgtatctcc gcgttagcgg gccgtcaggg ccgcttacga gcgcgtattc
    22321 ggaacatcca gccagaagac tgacagcgat gactatgctt agttataaca ttcataatta
    22381 aaagcgatgt tgttcccgcc cttcggacgg gggcggcctc gcttttcagt tatgaggggt
    22441 gggcacggtg ctgcgtttgt gtggtgtttt tattcctgat gttttttgtt gtggtttcag
    22501 accggtgagg ttcggggccc gcgcgttagc gtgggaggtg cttttagatt ctgagcggcg
    22561 cggccttggc cgctgcaagc gaaggcctct ttgtatttgt tcacagtggt ttcagagata
    22621 aagactttcc ctgcggcgaa gcaacgcagg ggattaaata atgaggttaa gactatattg
    22681 cagctttgct gtttcaagtt tgcagcttct taaagttctt tgtttgcgga tttatatgga
    22741 taactgaaac gacgtggcca cgttctgatg actgatgcgt agttttatgg gcatataggg
    22801 gtcagtttgg atatagagaa ttattgtacg gcaagcctgt tttttgaaca gacttgcctg
    22861 tcgcaataga atgtactccc gtgctatacg gcgtttgcag ggaatttgac cttaatcgga
    22921 taacctaaag tgcgacgact taaaaagcga tagccgctga cgcaaaatca atccgttacg
    22981 cttctcgatt gattaaataa tgctcttaac gtacaaaatt ttccgatatc caaaccgcca
    23041 ccgagcctag tggtttaggt agttttcttg ttgcgttaca ccaggtaatt gaggcgcgaa
    23101 gaagggattt ctgaagctgc caggaaatct ggccttgccc gccagagtat ttacccgcat
    23161 tatccccgaa tggaaccccg accatcacaa cacttgcaca actaacatct ggaaccttat
    23221 ttaaatgaca ccaaaacatt gataacaggg ctccctaacc ggagccctga tgagtcagtt
    23281 ttgtaccgta actttattac ccatcactga gatcaaaacg gaatccgcaa cagctttacc
    23341 tccagtcgtt tcacctccaa taatcaataa gctgtcattc cagggtaatg acactccgta
    23401 ggctcgcccc tgtggtagtt cacctgattt accccatttt ccgttatacc agagataaat
    23461 atcagcactg taggattttt taagaccttc atgcgcataa ttcttaccat tctggtaatt
    23521 tttccgcgaa ccttcgaagc cagctcctcc ggcaaatata agcgcatcat gacttattcc
    23581 tgcaaagcta cctgcaacgc catcaggtga tgagacggaa ggtagtttac tccagtttac
    23641 ccctgtgttc gtaaagtcca gttcaaacac gctatcggtt cgtagcccgg gtttgacttc
    23701 gccattaata agccaggttt tatcaccgga actcactgct gctgcaccag ccgtaccata
    23761 ccaaggggat tcaccagcgt aacgccattg ctgtgtcgca gggtcaaaag acaacagaaa
    23821 cttattaaag aaataatctt ctgacttttt attgaaataa ttagcattaa tcttatctgt
    23881 tgtttttgaa tcttttccgg cttggttaag atcctcaaag tatccattaa atatattctg
    23941 gttaacgcca cctgtaatat aagctttacc gttgtggaca aaggtcacat gccccgccat
    24001 ccccattggc gcatgcgaca tcaatttaac ccaggtattg ttttttgggt tatatttatg
    24061 gacgtcattg aatacctgtg ttaatccctc attgttttta ccaacgccgc caaatacata
    24121 caggttacca tcaataaaaa cagaggttgc ctgatctcgc gccccccccg ggaactcagc
    24181 caaggttgtc cattttttat ctttagcctg agtatccagt ttgtaccatg cggaaccagc
    24241 gctacctaaa ccaatgtaaa ctgtgttatt atcaattgca ccagtaccac ttttaaacgg
    24301 tactggtgtt tccggtagta cggacgcgtt tgccgcaaat gaactcatca tgacagtaag
    24361 cgctgttatt gttttattca ttgtaacagt ctcccgttcg ttacagtttt attgacacac
    24421 caatacgata actattttca gataaattat ctttgccgtt gtaaacaccc tgacggtcaa
    24481 ggtaatcata ttctatatat ggcgtaatat cgggtgtcat atggtattgc agaacaaatg
    24541 cattttcagt cgcatctttc ttatggttac catagcgata attgttttgt ttgctataaa
    24601 cagttgtttg ccatgcaaag gtaaagtctt tatttatttt atacgttaaa tatccatccc
    24661 aacgatgcgc attgtctcga tccattttgc cagaaaggtc ttcctgtctg taggttttcc
    24721 agtcataccg gtaacggatc ccaaggttca aatcttttgt aacatcccac gaaagtttaa
    24781 tatatggtcc atagcgtgtg ccattactgc tgaagtgtgt cagcatccct ggacgaacag
    24841 accatttatc atcaagttta atagcataat taatttctgc ttggacatca ttcagtgcaa
    24901 gattttcttt tttattatca tggattgtat tccacgtatt actttcaaga cttgcccacc
    24961 atccattctg ccacccttcg ctcactttta atcgggtttc ataggcatga ctgccacttc
    25021 tatatcctcc ccggatatcc agtgttgcag cattagatat aacagaaaat gaggcgcaca
    25081 ataatatacc agaaactact tgatatcttt tcatatatat accttttgtc gaacacatga
    25141 ttagttatgt ggagctataa aatcaacata attctttata aaaaatgtcc tttttattat
    25201 ttttctgcca gtaatgcagc accaattatt cctgcattgt tacggtaagt ggcaggaact
    25261 tcccggagtg ggaatttcac gccgggatga aatgttaagg gtatcatcaa ccagtgcagc
    25321 tgccagtttc gtaccaccga tatctattgc cagagtacgc attatctgag agcctcctga
    25381 atggctttac aataccagtg acagatgtgt tcctgatgag taatggcagc ccccactgtt
    25441 acagcccagg ttccattgct gcagccaaag ccgggggctt atcagtaata acgcggcaac
    25501 ctttcttgct taatctcctg actaaatcca gatagggagt atgatcacct gtgtaaccag
    25561 acaggctggt tacaataatt tctgtgccct gtctgtaaca tttcgaacca tcctgttctg
    25621 aagaacagtc agccattgcc gtgatcatct ctggatcatc cattggagca gcctgaacaa
    25681 agtggcagga aacgatcacc catccctgtt tttttacttt ttcgtccata agctgtggta
    25741 atgtcataac tactccgttt gttacagggg tcagcgggat ttcatttcca gagaagactc
    25801 atttttaatc acaccggcac cactgaatgg cttcccgtca atagcatcat gtgtacgcag
    25861 ggcttcgggt cttaaccagc gctgcatccg ggaaggcata tccagaccta tcagcaggat
    25921 gactacaaag gtcagaccga acgagagcga acacagtgct gttccgagat caaggcgttg
    25981 agcaacaacg gcccccagta caggagcaac tgctcccccc agagcgccta cgttataagt
    26041 gaatccaagg ccggcagcac gctgatcagt atcaaaataa ccgccaataa gttttggaag
    26101 aataccggca ataccttgcc ccaacatttg ctgaaaaaag agtaataaac ccagtaccca
    26161 tacactggat gcaccaatgg taaagacagg aatgatcaga atctgggaag ccagcagact
    26221 aaatacatag gctttacggg ttccaagcca gtctcccaga aaacctccga cgcaacatcc
    26281 agctgcagca ccaaatccac tgaaaaaaag aacctgagcg actgtggaag ggtcatatga
    26341 caactctgtt ttcagatatg tgggtaacag agcctgaatt ggccaggaat aaagaaacgc
    26401 aaacaggaca acgatcatga gcatgacgcc tgttggccag cgtttaccac tactctgaac
    26461 cataaatgaa ataaagataa ctgcacacaa aacgcccaga ccagcaatta tcagagcatt
    26521 ttgtaggttc ccggcaaaac agaaccaaag agcaacagca gcaaccagag tcatggcaat
    26581 atttgcaatc cgatattcac cacggtacaa aatatccacc attgtacgga caggtgcttt
    26641 cccttcatgt ttttctttcc agtcctctgc ttccggaata tttttacgca gccagagagc
    26701 aaaaataata ggaagaatcc ctataaagaa cagcactcgc catccccata ctggtacaac
    26761 cagactataa acctgagctg caacaactgc acctactgaa aagccagaaa tcagaaagcc
    26821 acttgcttta ttgcggagat gctttggcca gctttcgatg acgtatgtta cactactgcc
    26881 atattcacct gccatcccca taccaataac cagacgagca ataaagaggg ttatatatcc
    26941 ctgagcaagg ccacacgcca gtgttccaag ggaaaaaaga ataatactga caaccatcgc
    27001 cagacggcgt ccatatctgt ctcccattgc tcccagaaca agtccaccaa accaacggga
    27061 aataaatgca gcggatataa gactggctgc ctcgaccgtt gtcaggccaa agtctccctg
    27121 aatctcggta agaacaagag ttatcaacac aaaatcgaaa ccatcgagta aataacctag
    27181 ccaggcggca gaaaatgcac gccactggct acgatttaaa tgcttatacc atggcgtttt
    27241 attttgtagg gtattcataa ataacaacct cacatacaga tgttcctgtt tattcagtta
    27301 tcttaaactt tatataacaa agaatatgta accgtacttt atgttttttc tttcattaaa
    27361 gtttctgcca gagatttaag ttcaggcata tactctgcat taacaccaga gaatggtttt
    27421 ctgcataatg gggcatcaat gattcccata tagtgaagta ctgtttttaa tccccggaac
    27481 acaccaaacc tgattaaaag atcaataact ttattacatt cactctgaag atattttgcc
    27541 gtttttatat caccattctg cagtgcacca gctattcttt tatatctcca tcccataatg
    27601 ttatatgtac tacctatccc gccatctgca cctgccagca atccggatgc aaatatctca
    27661 tcataaccat tgtaaagaac caagtctgga tgagaccgac gaatctgctc catctgatat
    27721 aaatctccgg aggtttgttt aagtgctcta acaccgggta tagtcaccag tgtgctaatt
    27781 tgctcgagag ataactttac tccactcagt gcagggatgt tatataccac catcggtaat
    27841 ccatctgcgg catcaataat ggcacgataa tgttcacaat gctcttcaaa agaaaaggga
    27901 taataaaaag gtgtcactgc tgaaacagca tcatagccca gtctgcttgc tgcccgggcg
    27961 agactctgac tttcgtttgt actcacacaa ccaacgtgag caatcaactt cagttttcct
    28021 tttgcttcat cagcaacgat ctccagcact gactcacgtt cctgtacact ctgtacaaag
    28081 gcttctcccg tagagccacc aacatacaat ccatcaatcc cctgctctat attaaatctt
    28141 gtcaacctgc gcagactgtc ttgatccagc ttttgttctg catcaacagg tgtcagcaga
    28201 gcagacataa tgccttttaa atcggcagtc attgtttacc tcttcaacaa ttcagtatac
    28261 ctttatacct attacaccag tggttctgta caaatccata tggaaaaaat ggttttgtga
    28321 taatggttac aaaatttgtc tgccagttgc cagaaacgta caaatatatc aatgcaaata
    28381 ttgaaatctc atctggataa aacaatttaa atcgaccctc aggccaattc taaacattag
    28441 catatctaat gatttgttat tttatacttg tataaccgtt gtataaggct ggggttccgg
    28501 aatgatatta ataatgatga ctgttatttg tagtttgaat ttttagccac gatgaacgag
    28561 aatattaatg cataaactat gctcaatcag atgaatgatt cagtctctga tgtatctgta
    28621 aaattatatt ttgtttcatt catgtaactt aagtgttgat ttttaaactg gtaaattatg
    28681 tttgataaac gaccgttaaa aagaaaaaaa ctctctgagt tagttgagga agaactggag
    28741 tttatgatca gaaaaggaat ttttcaggag ggtgaacata taccgtcaga gcggacactg
    28801 atggaaatgt ttaaagttgg cagaccatct gttcgtgaag ccttggctac tttacgacgt
    28861 aaaggactta ttgaagttaa taatggtgaa agaacgaagg tttgtcgacc cactcctttt
    28921 actcttataa atgagttatc tgggattgcc agagattatc tggccagcga cgagggaatg
    28981 caacattttg aacaattacg actttttttc gagttaatgt tagtcagata tgcttcggtt
    29041 tatgcaaaca aggagcaaaa aaaataaact taatcaggct gcaaacattt gttggaaata
    29101 tgttgatgat aatatcaact ttattcgtgc agatattaac ttccatcggg ttttatctga
    29161 aatccctgaa aaccctgtat ttgttgctat tcatacagca ctaacagact ggttgataaa
    29221 tacacattct gaagagcaca gtaatataac ac
//
LOCUS       DAZFVO020000050        28993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFVO020000050 DAZFVO020000000
VERSION     DAZFVO020000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.050"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(72..1736)
                     /gene="glnS"
                     /locus_tag="ACHKNB_RS10250"
                     /old_locus_tag="pgaptmp_002050"
     CDS             complement(72..1736)
                     /gene="glnS"
                     /locus_tag="ACHKNB_RS10250"
                     /old_locus_tag="pgaptmp_002050"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HGU4195561.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPLL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1939..3885)
                     /gene="nagE"
                     /locus_tag="ACHKNB_RS10255"
                     /old_locus_tag="pgaptmp_002051"
     CDS             complement(1939..3885)
                     /gene="nagE"
                     /locus_tag="ACHKNB_RS10255"
                     /old_locus_tag="pgaptmp_002051"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HGU4195562.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4218..5018
                     /gene="nagB"
                     /locus_tag="ACHKNB_RS10260"
                     /old_locus_tag="pgaptmp_002052"
     CDS             4218..5018
                     /gene="nagB"
                     /locus_tag="ACHKNB_RS10260"
                     /old_locus_tag="pgaptmp_002052"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4195563.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5078..6226
                     /gene="nagA"
                     /locus_tag="ACHKNB_RS10265"
                     /old_locus_tag="pgaptmp_002053"
     CDS             5078..6226
                     /gene="nagA"
                     /locus_tag="ACHKNB_RS10265"
                     /old_locus_tag="pgaptmp_002053"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4195564.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6235..7455
                     /gene="nagC"
                     /locus_tag="ACHKNB_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /gene_synonym="nagR"
     CDS             6235..7455
                     /gene="nagC"
                     /locus_tag="ACHKNB_RS10270"
                     /old_locus_tag="pgaptmp_002054"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HGU4195565.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7503..8255
                     /gene="nagD"
                     /locus_tag="ACHKNB_RS10275"
                     /old_locus_tag="pgaptmp_002055"
     CDS             7503..8255
                     /gene="nagD"
                     /locus_tag="ACHKNB_RS10275"
                     /old_locus_tag="pgaptmp_002055"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HGU4195566.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8512..10176
                     /gene="asnB"
                     /locus_tag="ACHKNB_RS10280"
                     /old_locus_tag="pgaptmp_002056"
     CDS             8512..10176
                     /gene="asnB"
                     /locus_tag="ACHKNB_RS10280"
                     /old_locus_tag="pgaptmp_002056"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HGU4195567.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10557..10633
                     /locus_tag="ACHKNB_RS10285"
                     /old_locus_tag="pgaptmp_002057"
     tRNA            10557..10633
                     /locus_tag="ACHKNB_RS10285"
                     /old_locus_tag="pgaptmp_002057"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10591..10593,aa:Met,seq:cat)
     gene            10642..10726
                     /locus_tag="ACHKNB_RS10290"
                     /old_locus_tag="pgaptmp_002058"
     tRNA            10642..10726
                     /locus_tag="ACHKNB_RS10290"
                     /old_locus_tag="pgaptmp_002058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10676..10678,aa:Leu,seq:tag)
     gene            10750..10824
                     /locus_tag="ACHKNB_RS10295"
                     /old_locus_tag="pgaptmp_002059"
     tRNA            10750..10824
                     /locus_tag="ACHKNB_RS10295"
                     /old_locus_tag="pgaptmp_002059"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10782..10784,aa:Gln,seq:ttg)
     gene            10859..10933
                     /locus_tag="ACHKNB_RS10300"
                     /old_locus_tag="pgaptmp_002060"
     tRNA            10859..10933
                     /locus_tag="ACHKNB_RS10300"
                     /old_locus_tag="pgaptmp_002060"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10891..10893,aa:Gln,seq:ttg)
     gene            10949..11025
                     /locus_tag="ACHKNB_RS10305"
                     /old_locus_tag="pgaptmp_002061"
     tRNA            10949..11025
                     /locus_tag="ACHKNB_RS10305"
                     /old_locus_tag="pgaptmp_002061"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10983..10985,aa:Met,seq:cat)
     gene            11074..11148
                     /locus_tag="ACHKNB_RS10310"
                     /old_locus_tag="pgaptmp_002062"
     tRNA            11074..11148
                     /locus_tag="ACHKNB_RS10310"
                     /old_locus_tag="pgaptmp_002062"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11106..11108,aa:Gln,seq:ctg)
     gene            11186..11260
                     /locus_tag="ACHKNB_RS10315"
                     /old_locus_tag="pgaptmp_002063"
     tRNA            11186..11260
                     /locus_tag="ACHKNB_RS10315"
                     /old_locus_tag="pgaptmp_002063"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11218..11220,aa:Gln,seq:ctg)
     gene            complement(11414..12589)
                     /gene="ubiF"
                     /locus_tag="ACHKNB_RS10320"
                     /old_locus_tag="pgaptmp_002064"
     CDS             complement(11414..12589)
                     /gene="ubiF"
                     /locus_tag="ACHKNB_RS10320"
                     /old_locus_tag="pgaptmp_002064"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HGU4195568.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVMLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12735..14159
                     /gene="miaB"
                     /locus_tag="ACHKNB_RS10325"
                     /old_locus_tag="pgaptmp_002065"
     CDS             12735..14159
                     /gene="miaB"
                     /locus_tag="ACHKNB_RS10325"
                     /old_locus_tag="pgaptmp_002065"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HGU4195569.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14312..15352
                     /locus_tag="ACHKNB_RS10330"
                     /old_locus_tag="pgaptmp_002066"
     CDS             14312..15352
                     /locus_tag="ACHKNB_RS10330"
                     /old_locus_tag="pgaptmp_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGU4195570.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15349..15816
                     /gene="ybeY"
                     /locus_tag="ACHKNB_RS10335"
                     /old_locus_tag="pgaptmp_002067"
     CDS             15349..15816
                     /gene="ybeY"
                     /locus_tag="ACHKNB_RS10335"
                     /old_locus_tag="pgaptmp_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGU4195571.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15906..16784
                     /gene="corC"
                     /locus_tag="ACHKNB_RS10340"
                     /old_locus_tag="pgaptmp_002068"
     CDS             15906..16784
                     /gene="corC"
                     /locus_tag="ACHKNB_RS10340"
                     /old_locus_tag="pgaptmp_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HGU4195572.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16809..18347
                     /gene="lnt"
                     /locus_tag="ACHKNB_RS10345"
                     /old_locus_tag="pgaptmp_002069"
     CDS             16809..18347
                     /gene="lnt"
                     /locus_tag="ACHKNB_RS10345"
                     /old_locus_tag="pgaptmp_002069"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HGU4195573.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18425..20302)
                     /locus_tag="ACHKNB_RS10350"
                     /old_locus_tag="pgaptmp_002070"
     CDS             complement(18425..20302)
                     /locus_tag="ACHKNB_RS10350"
                     /old_locus_tag="pgaptmp_002070"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGU4195574.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPCAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20757..21665
                     /gene="gltI"
                     /locus_tag="ACHKNB_RS10355"
                     /old_locus_tag="pgaptmp_002071"
     CDS             20757..21665
                     /gene="gltI"
                     /locus_tag="ACHKNB_RS10355"
                     /old_locus_tag="pgaptmp_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HGU4195575.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDEPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21835..22575
                     /gene="gltJ"
                     /locus_tag="ACHKNB_RS10360"
                     /old_locus_tag="pgaptmp_002072"
     CDS             21835..22575
                     /gene="gltJ"
                     /locus_tag="ACHKNB_RS10360"
                     /old_locus_tag="pgaptmp_002072"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HGU4195576.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22575..23249
                     /gene="gltK"
                     /locus_tag="ACHKNB_RS10365"
                     /old_locus_tag="pgaptmp_002073"
     CDS             22575..23249
                     /gene="gltK"
                     /locus_tag="ACHKNB_RS10365"
                     /old_locus_tag="pgaptmp_002073"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HGU4195577.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23249..23974
                     /gene="gltL"
                     /locus_tag="ACHKNB_RS10370"
                     /old_locus_tag="pgaptmp_002074"
     CDS             23249..23974
                     /gene="gltL"
                     /locus_tag="ACHKNB_RS10370"
                     /old_locus_tag="pgaptmp_002074"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HGU4195578.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24092..25027
                     /gene="rihA"
                     /locus_tag="ACHKNB_RS10375"
                     /old_locus_tag="pgaptmp_002075"
     CDS             24092..25027
                     /gene="rihA"
                     /locus_tag="ACHKNB_RS10375"
                     /old_locus_tag="pgaptmp_002075"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HGU4195579.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25111..26781
                     /gene="hscC"
                     /locus_tag="ACHKNB_RS10380"
                     /old_locus_tag="pgaptmp_002076"
     CDS             25111..26781
                     /gene="hscC"
                     /locus_tag="ACHKNB_RS10380"
                     /old_locus_tag="pgaptmp_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HGU4195580.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26841..28292)
                     /gene="djlC"
                     /locus_tag="ACHKNB_RS10385"
                     /old_locus_tag="pgaptmp_002077"
     CDS             complement(26841..28292)
                     /gene="djlC"
                     /locus_tag="ACHKNB_RS10385"
                     /old_locus_tag="pgaptmp_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HGU4195581.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28289..>28993)
                     /locus_tag="ACHKNB_RS10390"
                     /old_locus_tag="pgaptmp_002078"
     CDS             complement(28289..>28993)
                     /locus_tag="ACHKNB_RS10390"
                     /old_locus_tag="pgaptmp_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4195582.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
BASE COUNT         7129 a         7534 c         7316 g         7014 t
ORIGIN      
        1 cgtttgggat tgtttgctat ttcttgccgg atgcgacgta aatgcctcac ccggcctggt
       61 agaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta
      121 aataccggtt tttccgctgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca
      181 aactggaatg ctttacccgc aaccgcatct ttcagcaacg gttcagcaaa gccctgtttg
      241 atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc
      301 acgctgaaca gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc
      361 cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg
      421 tcataagtac agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttctgcc
      481 ttaatgacgt aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc
      541 ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc
      601 tggcggctac ccatttccgg tttgttcgga tggttcggca tggtaaccat ttcgccttcg
      661 ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca
      721 ttttcgttga gatcttcacg gatgcaggat tccagcgatg ccatctcaat ggtgttgtcc
      781 tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagcagcagt gtaaccacga
      841 cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg
      901 gtcaccagca ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag
      961 aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg
     1021 cgacggttgt cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg
     1081 ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg
     1141 tgttcagcaa atttaatacg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc
     1201 atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt
     1261 tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt
     1321 tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc
     1381 aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag
     1441 taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca
     1501 acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct
     1561 ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga
     1621 tagccattcg gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga
     1681 tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg
     1741 gattcctcaa agcgtaaata acgtataacg gcgtatgatc ttataagtgg gacaggctga
     1801 caactgttag ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa
     1861 aaaaagcggc agagaagatc tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg
     1921 catcaggcat aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac
     1981 atggccctga gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg
     2041 gctaatcatc gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg
     2101 ccctgcgctt acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc
     2161 tacggtgtcg atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa
     2221 cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt
     2281 atctgtcggt ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg
     2341 aacctgatcc agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac
     2401 cgcgtttggt acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc
     2461 tgacgcagcg gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc
     2521 tgctttcgcg ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc
     2581 acccagacgt ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag
     2641 acgggtgata caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata
     2701 gttggtcgcc agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat
     2761 ctcgtcttct ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact
     2821 gaacaccacg aagtagatag cgaagaagac aacacccata accagcagca tccagacgtt
     2881 ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga
     2941 gaagccagca tggataccca gcagtgttgc cacaaacagg ctgataccgg tcagcagtgc
     3001 gtgcaggagg tacagcagcg gtgcaaggaa catgaacagg aattccagcg gctcagtcac
     3061 accggtcagg aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg
     3121 ctctttcggt gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcataat
     3181 cgggaagaag ccggacatga acatccccgc ggtgccgtca ccggcataga agcggttgat
     3241 atcaccgtgg aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat
     3301 ggtgttcagc acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat
     3361 accggaaccc agcgcgcccg cagaaacgat ccactcgcct cctgcatgga tagcgtgctg
     3421 taccggcggc cagacgtaac caaaaatggc cgccagcacc aggcagaaga agccggtggc
     3481 aatcggcaca aagcgtttgc cgccgaagaa actcaggaag tccggcagtt taatatcgga
     3541 ccaacggtta taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat
     3601 gttaatttct gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc
     3661 cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa
     3721 gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag
     3781 taaatctggc tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag
     3841 ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg
     3901 agaaccctat ttggctcgtt taaagccgta tttttatttt gctgcaaatt gtactgccga
     3961 tgttctgtaa tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgtat
     4021 cgcaaattaa acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc
     4081 ttttactggc taaagcagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc
     4141 ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt
     4201 acttattgag gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat
     4261 gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa accgactgcc gatcgtccgt
     4321 ttgttctggg cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa
     4381 tgcataaagc aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg
     4441 tcggtctgcc gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg
     4501 atcacgttga tattccagca gaaaacatca accttctcaa cggcaacgcc ccggatatcg
     4561 acgccgaatg ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta
     4621 tgggcggtgt aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt
     4681 ctcgtactcg tatcaaaacc ttgactcatg acactcgcgt cgcgaactct cgtttctttg
     4741 ataacgatgt caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acattgctgg
     4801 atgctgaaga agtgatgatt ctggtgctgg gtagccagaa agcactggca ctgcaggctg
     4861 cagttgaagg ttgcgtgaac cacatgtgga ccatcagctg tctgcaactg catccaaaag
     4921 cgatcatggt gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt
     4981 tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc
     5041 ttgctcaggg caatattttt taaaatcggg ggtcagaatg tatgcattaa cccagggccg
     5101 gatctttacc ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat
     5161 taaaagcgtc tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg
     5221 ggccattctc tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt
     5281 taacgacacc gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga
     5341 gaaatcaggc tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa
     5401 acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct
     5461 gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt
     5521 gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa
     5581 agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg
     5641 gattgtggtt tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg
     5701 cgcggggatt acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga
     5761 accgggcctg gcgggcgcga tcctcgacga agctgacatt tattgcggta ttattgctga
     5821 tggcctgcat gttgattacg ccaacattcg taacgctaaa cgtctgaaag gcgacaaact
     5881 gtgtctggtt accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc
     5941 gggtaaaaca atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg
     6001 ttcatcctta accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact
     6061 ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg
     6121 tctcggcaca ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa
     6181 aatcaccaag accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca
     6241 ccaggcggac aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg
     6301 gtttatcgcc tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc
     6361 cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc
     6421 aaagaagttg atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa
     6481 acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg
     6541 tttgatctca gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag
     6601 caaacgctgg aacatgccct gttgaatgcc attgctcagt ttattgatag ctaccagcgc
     6661 aaactgcgcg agctgatcgc gatttcggtg atcctgccag ggcttgttga cccggacagc
     6721 ggcaaaattc attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg
     6781 gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg
     6841 gagcactact tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc
     6901 ggaaccgggg ccgggattat ctctaacggg cgcattttta ttggccgcaa cggcaacgtc
     6961 ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt
     7021 ggctgcctgg aaactatcgc tgccaacgcc gctattgaac aacgggtgtt gaatctgtta
     7081 aagcagggct accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa
     7141 gccgcgaaca aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg
     7201 ggcaaaacca tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt
     7261 gaaatcaccg aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccag
     7321 gcgctgaagg cgtttcgcac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg
     7381 gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat
     7441 ttgctggaaa attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt
     7501 ccatgaccat taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg
     7561 ccgtaccggg tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt
     7621 tgctgaccaa ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg
     7681 gtgtcgatgt gccggatagc gtgttttata cctctgcgat ggcgactgcc gatttcctgc
     7741 gtcgccagga aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt
     7801 acaaagccgg tttcactatt accgatgtga accctgattt tgtgattgtt ggcgaaacgc
     7861 gttcctacaa ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct
     7921 ttatcgccac caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt
     7981 gtgcagggat tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt
     8041 ggatcatccg cgcggcatta aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg
     8101 gcgataactt gcgtaccgat attctggcgg gcttccaggc gggtctggag acgattctgg
     8161 tgctttctgg tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga
     8221 tttacccgtc ggtcgctgaa atcgacgtta tctgaatagc aaccacgtct cagggcgtgg
     8281 tttcttattt ttacccatca ttaaaatcat cccattcaat aaaactcgcc aaaaattatc
     8341 gatccatcaa taaatacatc accacaccat cccctttttc acaaatcagc aatcaccatt
     8401 acgtttttta tttttgcact gccaaatcgc ttgcgcacgg tgctgctttg cggcattttt
     8461 ttaaacaagc aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca
     8521 atttttggcg tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag
     8581 ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac
     8641 gccattctcg ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc
     8701 tacaaccaac aaaaaaccca cgtactggcg gtaaacggtg aaatctacaa ccaccaggca
     8761 ttgcgcgctg aatatggcga tcgttatcag ttccagacag ggtctgactg tgaagtgatc
     8821 ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc
     8881 tttgccctgt acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc
     8941 atcccactgt atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa
     9001 tcgctggtgc cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagc
     9061 caggacggcg aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa
     9121 gataacgtga ccgacaaaaa cgagctgcgt caggctctgg aagattccgt taaaagccat
     9181 ctgatgtctg atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt
     9241 tccgctatca ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc
     9301 tggtggccgc agttacactc ctttgctgta ggtctgccgg gttcaccgga tcttaaagca
     9361 gcccaggaag tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag
     9421 gaaggtctgg atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact
     9481 attcgcgctt caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa
     9541 atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccataaa
     9601 gcgcccaacg ctaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg
     9661 tatgactgcg cgcgcgccaa caaagcgatg tccgcctggg gcgtggaagc gcgcgttccg
     9721 ttcctcgaca aaaaattcct tgatgtggcg atgcgtatta acccacagga taaaatgtgc
     9781 ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcctgca
     9841 agcgtggcct ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac
     9901 accctgaaag aagtggcggc gcagcaggtt tctgatcaac agctggaaac tgcccgcttc
     9961 cgcttcccgt acaacacgcc gacgtccaaa gaagcgtatc tgtaccggga gatctttgaa
    10021 gaactgttcc cacttccgag cgccgctgag tgcgtaccgg gcggtccttc cgtcgcgtgt
    10081 tcttccgcta aagcgattga gtgggatgag gcgttcaaga aaatggacga tccgtctggt
    10141 cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa
    10201 atccggggct gtttcagtta ttgaggtgct tgttaattct gatatgcagt aaggaaatgt
    10261 tccgggtttg ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt
    10321 aatttcccac cactattgtt gaattccgaa tttcatcctg tacgacaaaa gaatggctgt
    10381 agggtgggga aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc
    10441 aacctaacca aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag
    10501 gctctatacg cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct
    10561 acgtagctca gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc
    10621 gtcgtagcca ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt
    10681 tctggcgccg caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg
    10741 ttgtacggat ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg
    10801 gttcgaatcc aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg
    10861 gggtatcgcc aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca
    10921 ggtaccccag ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca
    10981 ctcataatga tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc
    11041 gaatacgttc ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc
    11101 ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaat ttattcaaga
    11161 cgcttacatc gtaagtgcgc ccagttgggg tatcgccaag cggtaaggca ccggattctg
    11221 attccggcat tccgaggttc gaatcctcgt accccagcca cattaaaaaa gctcgcttcg
    11281 gcgagctttt tgcttttctg cgttcattca atgtcgaatg cgatgttgac acgtcttatc
    11341 cttcaatgtc ggatgcgacg ctgccgcgtc ttatccgacc tacggttggc acgcatccgg
    11401 caatgttgta aggctacaac cctaacgcat atttcagcgc ctgacgtttc aacacgccag
    11461 cacgctctgc cgccattaac ccgagattac gcacaaaacg cagcggcggc agattattac
    11521 tgaatccggc ataaaacaga tccataccac tttgcataat gaagttatcc gccatgcgcc
    11581 gcatctggta acgcttgagg acaggataac tggcccacgc ttcaccgtag ctgcgggcat
    11641 tcaccagaac atcaatcagg gcatcgacat cacgataacc aagattcacc ccctgccccg
    11701 ccagcggatg gatggtatgc gcggcatcgc ccaccagcgc aagccccggc tgcacatact
    11761 gcaacgcatg tcggcgcgtc agcggaaacg caccagcggc aagcggtgta acgtaaccca
    11821 gacgcgacgg gaaatgcttc gcgatttccg cctggagttg cgccatattc atattctgca
    11881 actggcgaat acgtaccgga gagtcatacc acaccagcga tgcccagtta tcaaacaacg
    11941 gcagaaacgc acgtggtccg tccggggtaa attgctgcca ggtgctgtcg ccgggatcgt
    12001 tctcacactg tacgctaatc aacatacacg actgcgcata ctgccaggca tgaacgccaa
    12061 ttcccgccat ctgccgcacc tgcgaatttg cgccgtcagc accaatcacc agcttcgcgc
    12121 gaatcacttc accgcccttc agttccagct cctgaagatc attatctcga tgtaatgcaa
    12181 tcagcgagcc tggcacacgt aacattactt tcggatgcgc ttccagcgcc tgccacaatg
    12241 cctgttgcag gacggtgttt tccaccatat agccaagcag cggcagctta agttcagcgg
    12301 cgtcaaacat cacatgcgcc gtttcccact cccacgtttc cagtctgcgg taaggatggc
    12361 aacgcatagc ctgtactgca tcccagaccc ctaacccttt aagcaatgat accgatgcgg
    12421 cgctgatcgc cgagatccgc acgtccggtt ggctatcagc gacaaacggt gcaggttctg
    12481 cgtgctcgat caccgttacc gaaaatccgt gctgtgccag ccccagcgcc agtgcgccgc
    12541 cgaccattcc tccgccgaca atggcaattt ccgttggttg atttgtcatg gtcaatcatc
    12601 ctgttagcaa tatgctttaa gtttacagga tttttacccc caagggctga taacgctcat
    12661 actggtcaca acggcagcaa agcattacac tatgcgccct gcattcctgg ctactatttc
    12721 gcaagagcaa gtcgatgacc aaaaaactcc atattaaaac ctggggctgt cagatgaacg
    12781 agtacgattc atcgaagatg gccgatctgc tggatgccac ccacggctat caactgaccg
    12841 acgtggcgga agaagcggat gtgctgctgc tgaacacctg ctcaatccgc gagaaggctc
    12901 aggaaaaagt cttccatcag ttgggtcgct ggaaactgtt aaaagagaag aatccagacc
    12961 tgattatcgg cgtcggtggc tgcgtggcat cgcaagaagg cgagcacatt cgccagcgcg
    13021 cccactatgt cgatattatt tttgggccgc aaacgctgca ccgtctgccg gagatgatca
    13081 actccgtgcg cggcgaccgc agcccggttg tagatatcag cttcccggaa atcgagaagt
    13141 ttgaccgtct accggaaccg cgcgccgaag ggccgaccgc gtttgtctcc atcatggaag
    13201 gctgcaataa atattgtacc tactgcgtgg tgccttacac ccgtggtgaa gaggtaagcc
    13261 gtccgtctga cgatattctg tttgagattg cccagcttgc tgctcagggc gtgcgtgaag
    13321 tcaacctgct cggtcagaac gtgaacgcct ggcgtggtga aaactacgac ggcaccaccg
    13381 gatcgtttgc cgatctgctg cgtctggttg ctgcgatcga cgggatcgat cgtattcgct
    13441 ttaccaccag ccatccgatc gaattcaccg acgatatcat cgaagtgtac cgcgacacgc
    13501 ctgagctggt gagtttcctg catctgccgg tacagagcgg ttccgatcgc attctgaacc
    13561 tgatgggccg tacccatacg gcgctggagt acaaagcgat catccgtaaa ctgcgtgcgg
    13621 cgcgtccaga tattcagatc agttctgact tcattgttgg cttccctggc gaaaccaccg
    13681 aagacttcga gaaaacgatg aagctgattg ccgacgtcaa tttcgacatg agctacagct
    13741 ttatcttctc tgcacgtccg ggtacaccag ccgccgatat ggttgatgat gttccggaag
    13801 aagagaagaa gcaacgtctg tatattctgc aagaacgtat caatcagcaa gcgatggcat
    13861 ggagccgccg gatgctcggc accacccagc gtattctggt agaagggact tcgcgtaaga
    13921 gcatcatgga gctttccggg cgtaccgaaa ataaccgcgt ggtcaacttc gagggcacac
    13981 cggatatgat cggtaaattc gtcgatgtag aaattaccga cgtctacccg aactctctgc
    14041 gcggtaaagt ggtgcgtact gaagatgaaa tgggtctgcg cgtggcagaa acaccggaat
    14101 cggtgattgc ccgtacccgc aaagaaaacg accttggcgt gggttattat cagccgtaat
    14161 tctcaggccc gccgttccgg tgggccttgt tttttctctc tttatcccca tctttatcgc
    14221 aatgcttgcg cctttttcct gtggcgtgaa taatttcctt ataggccggt gaatgttcag
    14281 cttccggcga catacagata agaggaacgg tttgaacata gacactcgcg aaatcaccct
    14341 ggagccagca gacaacgcgc gtctgttgag cctgtgcggc ccgtttgatg acaacatcaa
    14401 gcagctcgaa cgccgtctcg gcatcgagat caatcgccgc gacaaccact ttaaactgac
    14461 cggccgtccg atttgcgtca ccgctgcggc agacattctg cgtagcctgt atgtcgatac
    14521 agccccgatg cgcggtcaga ttcaggatat cgaaccggaa cagatccacc ttgcgattaa
    14581 agaagcgcga gtactggagc aaagtgcgga gagcgtgccg gagtacggca aagcggtcaa
    14641 tatcaaaacc aaacgcggcg taattaagcc gcgcacgcca aaccaggcgc agtacatcgc
    14701 caatattctc gaccatgaca ttaccttcgg cgttggcccg gcgggtacgg gtaaaaccta
    14761 cctggcagtg gctgcggcag ttgatgccct ggagcgtcag gagattcgcc gtattctgct
    14821 gactcgtccg gcggtcgaag ccggtgagaa actgggcttc ctgcctggcg atttaagcca
    14881 gaaagtagac ccgtatctgc gcccgctgta cgacgcgctg tttgaaatgc tgggctttga
    14941 gaaagttgag aaactgattg agcgcaacgt tattgaagtc gcgccgctgg cctatatgcg
    15001 tggtcgtacg ctgaacgacg cgtttatcat tctcgatgag agccagaaca ccaccatcga
    15061 acagatgaag atgttcctga cccgtatcgg ttttaactca aaagcggtta tcaccggcga
    15121 cgtcacacag atcgacctgc cgcgtaatac taaatcaggc ttacgtcacg ccatcgaagt
    15181 gctggccgat gtcgaagaga tcagctttaa cttcttccac agcgaagacg tggttcgtca
    15241 ccctgtggtg gcgcgtatcg ttaacgccta tgaagcctgg gaagaagccg aacaaaaacg
    15301 aaaagcggcg ctggcggcag aacgcaagcg cgaagaacag gaacaaaaat gagtcaggtg
    15361 atcctcgatt tacaactggc atgtgaagat aattccgggt taccggaaga gagccagttt
    15421 caaacatggc tgaatgcggt gatcccgcag tttcaggaag aatcggaagt gactattcgc
    15481 gtggtcgata ccgccgaaag ccacagtctg aatctgacct atcgcggtaa ggataagccg
    15541 accaacgtgc tctccttccc gtttgaagtg ccgcctggca tggaaatgtc gctactgggc
    15601 gatctggtta tctgccgtca ggtggttgag aaggaagctc aggagcaagg caaaccactg
    15661 gaggcgcact gggcgcatat ggtggtgcac ggcagtctgc atttgttagg ttacgatcac
    15721 atcgaagatg acgaagcaga agaaatggaa gccctcgaaa cagagattat gcttgctctg
    15781 ggctatgagg atccgtacat tgccgagaaa gaataatttg ccaggcattt gactggcaga
    15841 cgtccccgcc gttggtgatt aacgacggcg ttttgattaa ctaacatgag aacccatacg
    15901 acgccatgag cgacgacaat tcacacagta gtgacacgat aagcaacaag aagggatttt
    15961 tctccctgtt actcagccaa cttttccacg gtgaaccgaa aaaccgtgac gaactgctgg
    16021 cgctgatccg tgattccggg cagaacgacc ttatcgacga agatacgcgc gatatgctcg
    16081 aaggggtgat ggacatcgca gaccaacgcg ttcgcgacat catgatcccc cgctcccaga
    16141 tgattaccct gaaacgcaac cagacgctgg acgaatgcct tgatgtcatc atcgagtccg
    16201 cccactcacg tttcccggtg attagcgaag acaaagatca cattgaaggg attctgatgg
    16261 cgaaagactt gctgccgttt atgcgcagcg atgctgaagc cttcagcatg gacaaagtgt
    16321 tacgtcaggc ggttgtcgtt cctgaaagta agcgcgtaga ccggatgctg aaagagtttc
    16381 gctctcagcg ttaccacatg gcgatcgtta ttgacgaatt cggtggggtt tccggtctgg
    16441 tgaccattga agacatcctg gaactgattg ttggtgagat tgaagacgag tatgacgaag
    16501 aagatgatat cgacttccgt cagctgagtc gtcatacctg gaccgtgcgc gcactggctt
    16561 ccattgaaga cttcaacgaa gcgttcggca cccactttag cgatgaagaa gtcgacacta
    16621 tcggtggtct ggtgatgcag gcgtttgggc atcttccggc gcgtggcgaa actatcgaca
    16681 tcgacggtta ccagttcaaa gtggcgatgg ccgatagtcg gcgtattatt caggttcatg
    16741 tcaaaatccc ggatgactca ccccagccaa agctggatga ataaaaccga aactggatag
    16801 ataactacat ggcttttgcc tcattaattg aacgccagcg cattcgcctg ctgctggcgt
    16861 tattattcgg tgcctgcgga acgctggcct tctctcctta cgatgtctgg cctgcggcga
    16921 ttgtttcgct gatggggctt caggcgctga cctttaaccg ccgcccgctc cagtctgccg
    16981 ctattggctt ctgctgggga tttggcctct ttggcagcgg tattaactgg gtctatgtca
    17041 gcatcgcgac ctttggcggg atgcctggcc cggttaacat cttcctggtg gtgctgctgg
    17101 cggcgtattt gtcgctgtat accggactgt ttgctggcgt gctgtcgcgc ctgtggccga
    17161 aaaccacctg gctgcgcgtg gcgattgccg cccctgctct ctggcaagtg accgagtttc
    17221 tgcgcggttg ggtgctgact ggcttcccgt ggttacagtt cggctatagc cagattgatg
    17281 gcccgttaaa agggctggca ccaataatgg gcgtggaagc cattaacttc ctgctgatga
    17341 tggttagcgg cctgctggca ctggcgttag tcaaacgcaa ctggcgtccg ctggtggtgg
    17401 ccgtcgtgct gtttgccctt cccttcccgc tgcgttacat ccaatggttt accccgcaac
    17461 cggagaaaac cattcaggtt tcgatggttc agggcgatat tccgcaatca ctgaaatggg
    17521 acggagacca gcttcttaat acgctgaaga tttactacaa cgcaacggca ccgctgctgg
    17581 gcaaatcatc gttgattatc tggccggagt cggcgataac cgatctggaa attaatcagc
    17641 aaccgttcct caaagcactg gacggtgagt tgcgtgataa aggtagctcg ctcgtaaccg
    17701 ggattgtcga cgcgcgtctc aataagcaga accgctacga tacctacaac accatcatca
    17761 cactgggtaa aggtgcaccg tacagctacg aatcagccga tcgctataac aaaaaccatc
    17821 tggtgccgtt tggcgagttt gtcccgctgg agtcgattct gcgtccgtta gcaccgttct
    17881 ttgatctgcc gatgtcgtcg ttcagccgtg ggccatatat ccagccgccg ctgtcggtaa
    17941 atggtattga gcttactgcg gccatttgct acgagatcat tcttggcgag caagtgcgcg
    18001 ataacttccg cccggatacc gactatctgc tgactatctc caacgatgcg tggtttggta
    18061 aatctattgg tccatggcaa cacttccaga tggcgcgaat gcgtgcgctg gagctggcgc
    18121 gcccactgtt gcgcagcacc aacaacggca ttacggcggt gattggcccg cagggtgaga
    18181 ttcaggcgat gatcccgcag ttcacccgcg aggtgttaac cactaatgtg acgccgacca
    18241 ccggactcac accatacgca cgtaccggca actggccgct gtgggtgctg acggcactgt
    18301 ttggttttgc cgctgtgctg atgagtctgc gagcacgtaa acgttgatgt gcctgatgta
    18361 tcctgcatga gcaaaacgta ctttctcatt gagctttggg gcatggttat catgcccctt
    18421 tttcttacgg ttcagggata caagccacat tgccatattg gggatgacga ataattgcct
    18481 gcggatcaag cgagcgcgtc ctgtccagtt ggatcaaccc gttcttcgcc ataacaaata
    18541 tttttctacc atccgctgaa aaacgaaggt gattaacgta gctggcagga taaacgccaa
    18601 taatctgccc ggcattaata ttgagcagga tgacataacc ttgcaggcca tatgacacca
    18661 acgctaaata ctgaccgtcc gggctaatac cctcaacgga ataaacgctc ctgctacacc
    18721 attgcgtcat aatgctggct tgttgatccc ctacgcttat acgtgaaatc ccctggctgt
    18781 cggcaaaaat tacatccccg ttttcaggtc gaaaacgcat cgccgatatc gttcctcgcg
    18841 catcactagc cggatagctt tctttctgat gcgtgatggt attgatgcgt acaatgtcac
    18901 ctctgaccat accgggtatc aacaatattt tttcatccgg ggaaagagcc ataacccagg
    18961 cacaaggttt attactcgtc cccgtcccgt cgtatttctc cagcgggata tccgccacat
    19021 gctgataagt caccagatca atcgctgaca cactattact ggtgcgattc atcacccagg
    19081 cttttgtacc atcacgcgag aggataaggc tgtcaggatg tgcaccggtg gtaatcgact
    19141 gaataatctc ccctgtcgcc acgttaatga ccgataacgc attcgcttca aatgaagtcg
    19201 cataaatcaa cttttcatcc gggcttaatg ccagcgatct gacgccctga tctttacagc
    19261 caccacaatg tttgactgac ttttctttcg ctatgggcgc tgtaaaggta tgcaggattt
    19321 tcttttgtcc gaggtcgaaa acaacaatgc tgttggtgtt ttcaattgcg gcatacaaca
    19381 tattaccatc ccggctgatc gccatatcat ggatatcggc aatctcagcg agcaaatcac
    19441 cactggcctg ttcacgactt accggagcgg ataatgtttc cctgagggcg ttgcgttcct
    19501 ccgctaactg ctgctttttt tgccgtcgct cttgttcaat ctcctgtgga taaaacgctt
    19561 cacgcaggct ttgcctgact tgtaaaactg actcatccat ctgctgctgc gcaagccaga
    19621 tagccccggt cagaagaaga ctgcccgcaa caataatccc gccttccgta acgagtcggt
    19681 tttgcccaga taaacgcgcg ctcagccaac ccaacgcgcc acccgcaatc aggccgccaa
    19741 tgtggcaagc gttatctatt cctgtttgcc gggcaccgta caacagtgtc agcgccacca
    19801 tacccaacag aggaaatact cgccgctggt ttttatggta tgtgcccgta cctgaagcaa
    19861 actgtgttgc tatcgctgca ccagctattc ccagaatcgc cccggatgcc ccaacgctga
    19921 tgactatctg ctcactttcg cgtaacgcat aacaggcgct gatgagccca ccaaagacgc
    19981 cggagaataa ccaggtaatc aggagtcgcc atttccccag aatctgttcc gccacgcctc
    20041 ccacgaccag gaatgcaaac atattcatca gcagatgaga aacactactg tgcagaaaga
    20101 tactgctcaa taagcgccag ggttcacccg acaaagtaag aggcgcaacg tttgcgccga
    20161 gatggataag tgcggattga tcgacctctt cataactcat ccattgtgcg agataacaaa
    20221 atatcccaat acagagtgca aaaaggataa gggttattgc gggtaattga acatttaacg
    20281 gcttaaccga agatgcagac ataaggtgtt tcttccttga aaagacaggt aaaaagcatt
    20341 tttcccgtgt gcagttatca tcccggcaaa cgtcccatgc cgaaaagata attctgtccg
    20401 aaatatgact ttttcagcct aaaattacaa tcgtaattaa ttcgccccta agtatttttt
    20461 ggcatgtaag ttgctttatt tactgtgtaa gcgatgaaat cagccgcgac gcaaccaggt
    20521 tgcaccacca caaatcagcg gtagcaccaa aaaggtgcgg tttaagaatt ttgcttcgga
    20581 atggtgcggc acatctcgca aaaacaaaca ataaaacagc aatatcttta catttagcca
    20641 aactaaatgt tacctaacag gcacaacact gcacaataaa gttgcagacg ataacaacac
    20701 aaacactcac aacgggtatc catgcgttct taacgcagaa gataaaggag ttggatatgc
    20761 aattacgtaa acctgccaca gcaatcctcg ccctggcgct ttccgcagga ctggcacagg
    20821 cagatgacgc cgccccggca gcaggcagta cgctggacaa aatcgccaaa aacggtgtga
    20881 ttgtcgtcgg tcaccgtgaa tcttcagtac ctttctctta ttacgacaat cagcaaaaag
    20941 tggtgggtta ctcgcaggat tactccaacg ccattgttga agcagtgaaa aagaaactca
    21001 acaaaccgga cttgcaggta aaactgattc cgattacctc acaaaaccgt attccactgc
    21061 tgcaaaacgg cactttcgat tttgaatgtg gttctaccac caacaacgtc gaacgccaaa
    21121 aacaggcggc tttctctgat actattttcg tggtcggtac gcgcctgctg accaaaaagg
    21181 gcggcgatat caaagatttt gccgacctga aagacaaagc cgtagtcgtc acttccggca
    21241 ctacctctga agttttgctc aacaaactga atgaagagca aaaaatgaat atgcgcatca
    21301 tcagcgccaa agatcacggt gactctttcc gcaccctgga aagcggtcgt gccgttgcct
    21361 ttatgatgga tgatgccctg ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact
    21421 gggaaatcgt cggcaagccg cagtctcagg aagcctacgg ttgcatgttg cgtaaagacg
    21481 agccgcagtt caaaaagctg atggatgaca ccatcgctca ggtgcagacc tccggtgaag
    21541 cggaaaaatg gtttgataaa tggttcaaaa atccaattcc gccgaaaaac ctgaacatga
    21601 atttcgaact gtcagacgaa atgaaagcac tgttcaaaga accgaatgac aaggcactga
    21661 actaattaca agaaccaggg gcggaaattc cagccctctc gattgttacg tagcacggac
    21721 agactatacg cctgatggtc gttccccatc gggcctgaaa accgcaatac gctgggtaac
    21781 aatcttcgag ggtagcagtt aacgctgcta ccctcttttt tctggagtag atttatgtct
    21841 atagactgga actggggtat ttttttacaa caagccccgt tcggcaacac cacctatctt
    21901 ggttggatct ggagtggttt tcaggtgacg atcgctttgt cgatctgcgc ctggattatc
    21961 gctttcctcg tcggttcatt tttcggcatt ttacgtaccg ttccaaaccg ttttctctcc
    22021 ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc cgttgattgt gcaattcttt
    22081 acctggtatc tggtgatccc ggagctgttg ccggagaaaa tcggcatgtg gtttaaggcc
    22141 gagctggatc ccaatattca gtttttcctt tcatccatgc tctgcctggg gctgtttacc
    22201 gccgcccgtg tttgcgaaca ggtacgcgcc gccattcagt cgttgccgcg tgggcaaaaa
    22261 aatgccgcgc tggcgatggg cctgacgcta ccgcaggctt atcgatacgt actgctgcct
    22321 aatgcttatc gcgttatcgt cccgccgatg acttccgaga tgatgaacct ggtgaaaaac
    22381 tccgccatcg cctcgactat tggtctggtg gatatggcgg cgcaggcggg taaattgctt
    22441 gattactcag cccacgcctg ggagtcgttt accgctatta cgctggcgta tgtgttgatt
    22501 aacgcggtta tcatgctagt aatgacgctg gttgaacgta aagttcgcct gcctggcaac
    22561 atggggggca aataatgtac gagtttgact ggagttccat tgtcccttcc ctgccatatc
    22621 tgctcgacgg gctggtgatc acgctgaaaa tcaccgtcac ggcagtcgtg attggtattt
    22681 tgtggggcac catgctggcg gtgatgcgct tgtccagctt tgcgcccgta gcgtggtttg
    22741 ccaaagccta cgttaacgta ttccgctcga ttcctttagt tatggttttg ctgtggtttt
    22801 acctgatcgt gccaggtttt ctgcaaaacg tgctgggatt atcaccaaaa aatgatatcc
    22861 gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc ggcctactat tcagagatta
    22921 tccgtgctgg tattcaaagt atttctcgtg gtcagtcgag cgccgcgctg gcgttgggaa
    22981 tgactcactg gcagtcgatg aaactgatta ttctgccgca ggcgttccgc gcgatggtgc
    23041 cgcttctgct cactcagggc atcgtactgt tccaggatac ctcactggtg tatgtgttat
    23101 ctctggccga tttcttccgt accgcctcaa ccattggtga gcgtgatggt acgcaggttg
    23161 agatgatcct gtttgccgga tttgtttatt tcgttattag tcttagcgcg tcgttgttgg
    23221 tcagctactt gaaaagaagg acagcataat gattaccctg aaaaatgttt caaaatggta
    23281 tggtcacttt caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt
    23341 ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt aaaaccgtca acggcctcga
    23401 accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg gttaatgaca agaaaaccga
    23461 tctggcaaag ctgcgttccc gcgtcgggat ggtattccag catttcgagc tgttccctca
    23521 tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa gtgcttaaac gcgataaagc
    23581 gcccgctcgt gaaaaagcat tgaaattgct ggagcgcgtg ggactttccg cacatgccaa
    23641 taaatttccg gcgcagcttt ccggcggtca gcagcagcgt gtggcaatcg ctcgcgcgtt
    23701 gtgtatggat cctattgcga tgctgttcga cgaaccgaca tcggcgctgg atccggagat
    23761 gatcaacgaa gtgctggacg tgatggtgga actggcaaac gaaggaatga ccatgatggt
    23821 ggtgacccac gaaatgggtt ttgcccgtaa agtggcgaat cgggtgatct ttatggacga
    23881 gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg
    23941 cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc tggcgcgcat acccaattgc
    24001 gcgccatctc acgctttatc gtttcgcccc tcgtatctgt tacatcctgg cgttaccctt
    24061 gtcgcaaaga agcacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga
    24121 cccaggtcat gatgacgcta tcgcaatagt tctcgccctc gcctcaccag agcttgatgt
    24181 caaagcaatt acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct
    24241 gcgtatgctg accttgctta atcgcaccga tattccggta gccggcggcg cggtaaaacc
    24301 gttaatgcgt gagttgatta tcgcggataa tgtgcacggc gaaagcggac ttgacggccc
    24361 ggcactaccg gaaccgacgt ttgcaccgca aaagtgtacg gcggtggagc tgatggcgaa
    24421 aacgctacgt gaaagtgcgg aacctgtcac gattgtgtct accggaccgc aaactaacgt
    24481 tgccttgctg ctcaatagcc atccggaact gcatagcaaa attgctcgta tcgtgattat
    24541 gggcggcgca atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga
    24601 cccggaagcg gcagaaattg tcttccagtc agggatcccg gtggtgatgg ccggtctgga
    24661 tgttactcat aaagcacaaa tccacgttga agacaccgag cgtttccgcg cgattggcaa
    24721 ccctgtttca accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga
    24781 aaaatggggc tttgtcggtg caccgctgca tgacccatgc accatcgcct ggctgttgaa
    24841 acccgagttg tttaccactg ttgagcgctg ggttggcgtg gaaacacagg ggaaatatag
    24901 ccagggtatg acggttgttg attattatta tctgactggc aataaaccga atgccactgt
    24961 aatggtcgat gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta
    25021 cgcttaacac acgtcattga ttaagatcac cacgcgttac ctgggtggtc ttaatctgtt
    25081 tatctctttt tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc
    25141 gatctcggca ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt
    25201 ccaaataagt tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat
    25261 attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg
    25321 ttatttaaac gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac
    25381 gcgccagaac tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg
    25441 caacgtccga ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc
    25501 aagcataccc gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa
    25561 cccacagcag ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt
    25621 tttgatctcg gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt
    25681 gaagttcacg cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg
    25741 gtcgatgacg ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg
    25801 gcagccttgt acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt
    25861 cgctggcagt accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat
    25921 ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca
    25981 cgtctgaagc cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg
    26041 ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg
    26101 agcaccattg tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat
    26161 attgaagagg tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc
    26221 cagggcgttt ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg
    26281 cgtgtagaaa cttactcgac catgcacccg gaacaggatt caattacggt taacgtctat
    26341 cagggagaaa accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg
    26401 aagaaaaccg gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg
    26461 cttgaagttg acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc
    26521 ccggtaacat tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa
    26581 atttatccgc gcgatatgct catcaaccgc acctttaagg ccaaactgga agagttgtgg
    26641 gcgcgggcgc tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg
    26701 gcgttgcagt caaacgatat ggcccgcgtc gatgaggtca ggcggcgggc gagcgtttat
    26761 ttagccattg agacctcata atcacggctg ccggatgcgg cgtaaatgct gcatccggca
    26821 tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga ttttgccgag agcataacct
    26881 ataaccagca ccgttaaact gattttgata atgctggtaa tgctctgtgg tttttcaccc
    26941 tcatcatttt tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc
    27001 tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg cctctaatgg atgagcatct
    27061 ccgccttcca gccttgtatt aagacgggcc agcatctctt gtttcggaaa cgcttcaagt
    27121 actacctgtt ccgctacccg atacaaatcc agtaacggtg aatcttcttc tgtctcgcta
    27181 ccgactctcg atttccacca cgcccatgct ttgcgtttga agaataacga ctcaataaac
    27241 gattgaccta acggcgataa ttttccgcta tgtaaaattt ccgcccataa cagccgcgtc
    27301 cgggcgctct caccaggaac atatttcagc ggttgacctt gcgcatctac catgctccgc
    27361 gcttcgataa ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc
    27421 aacaacaaat tatcagcttg tgtagatggg taactttccc ggtacgcgca gagatcggca
    27481 agcaggcttt ccccctcgcc gcagtacaac ctttgagcac acaagtaata tcgggcatct
    27541 tcgctttctg gttcttccat ttgccactgt ttggctacag gaatgagttc cgccatacct
    27601 gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg
    27661 aaccacggac cttccatcgc caaaaacgca gtgacgtagt caggatggta acggcagcaa
    27721 cgttccagcg cgaagtaata ctcaacagtc tggttctgca acgcaacggg atattccaga
    27781 aggctaagga aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct
    27841 tcctcatcca ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccagcaaa
    27901 gaagcacaat ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt
    27961 aattgatcga catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg
    28021 gagggctgaa aacgatcact ttccgaatcc agtaatgtcc tgaatgcacg cagtatttca
    28081 tgttcagcgg cggcatcttt ttcttcatca tctgcttcct cgacaggatt taccgccagt
    28141 cgcagcgcct cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga
    28201 tgacataacg gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt
    28261 tcaatttcga ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg
    28321 ggcattcagg ctggacagtt aatggctgcc agggaagcgt ggcatagaaa cgatcgcttc
    28381 ccgctgccag ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg
    28441 cctcgcgtag cgaagtgttg tcttcgcact gagaagattg ccagtagaga cgtcctaacg
    28501 aataggcgga aaaatagtgc atccagctgt gatagtaatg gtgagctcgt acatagacgc
    28561 gcgattgcag ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg
    28621 acagaaaccc cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag
    28681 cggtttgggc aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg
    28741 ctttccactc ttcaggggaa gagcgatgcc atgtcagata taagccagct aaatcattgg
    28801 catgaccatc atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc
    28861 cccaggatga tgagagatcg aattctttta taccggtata catttttggc gagtcatgcg
    28921 cgggtttgca accctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt
    28981 gttcttcctt att
//
LOCUS       DAZFVO020000051        28834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFVO020000051 DAZFVO020000000
VERSION     DAZFVO020000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.051"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKNB_RS10395"
                     /old_locus_tag="pgaptmp_002079"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHKNB_RS10395"
                     /old_locus_tag="pgaptmp_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGU4195583.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKNB_RS10400"
                     /old_locus_tag="pgaptmp_002080"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHKNB_RS10400"
                     /old_locus_tag="pgaptmp_002080"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HGU4195584.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKNB_RS10405"
                     /old_locus_tag="pgaptmp_002081"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHKNB_RS10405"
                     /old_locus_tag="pgaptmp_002081"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HGU4195585.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDPQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMSEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKNB_RS10410"
                     /old_locus_tag="pgaptmp_002082"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHKNB_RS10410"
                     /old_locus_tag="pgaptmp_002082"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HGU4195586.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDT
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKNB_RS10415"
                     /old_locus_tag="pgaptmp_002083"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHKNB_RS10415"
                     /old_locus_tag="pgaptmp_002083"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGU4195587.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHKNB_RS10420"
                     /old_locus_tag="pgaptmp_002084"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHKNB_RS10420"
                     /old_locus_tag="pgaptmp_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HGU4195588.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11390
                     /gene="ftsK"
                     /locus_tag="ACHKNB_RS10425"
                     /old_locus_tag="pgaptmp_002085"
     CDS             7245..11390
                     /gene="ftsK"
                     /locus_tag="ACHKNB_RS10425"
                     /old_locus_tag="pgaptmp_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGU4195589.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQLYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQY
                     QQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            11545..12156
                     /gene="lolA"
                     /locus_tag="ACHKNB_RS10430"
                     /old_locus_tag="pgaptmp_002086"
     CDS             11545..12156
                     /gene="lolA"
                     /locus_tag="ACHKNB_RS10430"
                     /old_locus_tag="pgaptmp_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HGU4195590.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12167..13510
                     /gene="rarA"
                     /locus_tag="ACHKNB_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /gene_synonym="mgsA"
     CDS             12167..13510
                     /gene="rarA"
                     /locus_tag="ACHKNB_RS10435"
                     /old_locus_tag="pgaptmp_002087"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HGU4195591.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13601..14893
                     /gene="serS"
                     /locus_tag="ACHKNB_RS10440"
                     /old_locus_tag="pgaptmp_002088"
     CDS             13601..14893
                     /gene="serS"
                     /locus_tag="ACHKNB_RS10440"
                     /old_locus_tag="pgaptmp_002088"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGU4195592.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15132..17576
                     /gene="dmsA"
                     /locus_tag="ACHKNB_RS10445"
                     /old_locus_tag="pgaptmp_002089"
     CDS             15132..17576
                     /gene="dmsA"
                     /locus_tag="ACHKNB_RS10445"
                     /old_locus_tag="pgaptmp_002089"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HGU4195593.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17587..18204
                     /gene="dmsB"
                     /locus_tag="ACHKNB_RS10450"
                     /old_locus_tag="pgaptmp_002090"
     CDS             17587..18204
                     /gene="dmsB"
                     /locus_tag="ACHKNB_RS10450"
                     /old_locus_tag="pgaptmp_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HGU4195594.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18206..19069
                     /gene="dmsC"
                     /locus_tag="ACHKNB_RS10455"
                     /old_locus_tag="pgaptmp_002091"
     CDS             18206..19069
                     /gene="dmsC"
                     /locus_tag="ACHKNB_RS10455"
                     /old_locus_tag="pgaptmp_002091"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HGU4195595.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19105..19731)
                     /gene="ycaC"
                     /locus_tag="ACHKNB_RS10460"
                     /old_locus_tag="pgaptmp_002092"
     CDS             complement(19105..19731)
                     /gene="ycaC"
                     /locus_tag="ACHKNB_RS10460"
                     /old_locus_tag="pgaptmp_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HGU4195596.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     HLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20046..21194
                     /gene="ycaD"
                     /locus_tag="ACHKNB_RS10465"
                     /old_locus_tag="pgaptmp_002093"
     CDS             20046..21194
                     /gene="ycaD"
                     /locus_tag="ACHKNB_RS10465"
                     /old_locus_tag="pgaptmp_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195597.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21404..22834
                     /gene="ycaM"
                     /locus_tag="ACHKNB_RS10470"
                     /old_locus_tag="pgaptmp_002094"
     CDS             21404..22834
                     /gene="ycaM"
                     /locus_tag="ACHKNB_RS10470"
                     /old_locus_tag="pgaptmp_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4195598.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22835..23743)
                     /gene="ycaN"
                     /locus_tag="ACHKNB_RS10475"
                     /old_locus_tag="pgaptmp_002095"
     CDS             complement(22835..23743)
                     /gene="ycaN"
                     /locus_tag="ACHKNB_RS10475"
                     /old_locus_tag="pgaptmp_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4195599.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            23843..24433
                     /gene="ycaK"
                     /locus_tag="ACHKNB_RS10480"
                     /old_locus_tag="pgaptmp_002096"
     CDS             23843..24433
                     /gene="ycaK"
                     /locus_tag="ACHKNB_RS10480"
                     /old_locus_tag="pgaptmp_002096"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HGU4195600.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPFWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(24515..25255)
                     /gene="pflA"
                     /locus_tag="ACHKNB_RS10485"
                     /old_locus_tag="pgaptmp_002097"
     CDS             complement(24515..25255)
                     /gene="pflA"
                     /locus_tag="ACHKNB_RS10485"
                     /old_locus_tag="pgaptmp_002097"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HGU4195601.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(25447..27729)
                     /gene="pflB"
                     /locus_tag="ACHKNB_RS10490"
                     /old_locus_tag="pgaptmp_002098"
     CDS             complement(25447..27729)
                     /gene="pflB"
                     /locus_tag="ACHKNB_RS10490"
                     /old_locus_tag="pgaptmp_002098"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGU4195602.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(27784..28641)
                     /gene="focA"
                     /locus_tag="ACHKNB_RS10495"
                     /old_locus_tag="pgaptmp_002099"
     CDS             complement(27784..28641)
                     /gene="focA"
                     /locus_tag="ACHKNB_RS10495"
                     /old_locus_tag="pgaptmp_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HGU4195603.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7163 a         7095 c         7525 g         7051 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tggccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agctctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc actcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg ggatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgcccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccaa gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 tgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaat agtaatagcg agcagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggtatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca tcccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 agcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgctcacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgagga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cagcagatgt aatagcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc cagggatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac gcccatctga tcgccggtca cctcttccag cgtacggttg
     5461 gtgtgtagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt agcacaagca gaaactccac ggcctttaaa ggcttcttca
     5701 gatggcaggc cgagatagcg tgcagaagct ccagtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgc tatcgccagt cagacggaac ggtcgattct gcaaatcaac tttgttgatg
     5821 tgatcaaaga tgatttcagt ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgctgca
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 atagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagagtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctg aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatccggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggctggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagtact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgctgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg aatatgaaga tgacgaagag tatgaagatg
     7921 aaaaccacgg caaacagcat gaatcacgcc gtgctcgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgacgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattatgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccacttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgttgaaagc gccttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaagtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ctggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagagaa agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaactcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgttgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccgcagc agcagtatca gcagccacaa cagccggttg
     9601 cgccacagca gcaatatcag cagccgcaac agccggttgc gccgcagccg cagtatcagc
     9661 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
     9721 cacagccgca atatcagcag ccgcaacaac cagttgcgcc gcagcagcag tatcagcagc
     9781 cgcaacaacc ggttgcaccg cagccgcagt atcaacagcc gcaacaaccg gttgcaccgc
     9841 agcagcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
     9901 ataaaccgac gacgccgctg ccttctctgg atttgctgac accgccgccg agcgaagtgg
     9961 agccggtaga tacctttgcg cttgaacaaa tggctcgcct ggtggaagcg cgtctggctg
    10021 atttccgtat taaagccgat gtcgtcaatt actctccggg gccggttatc actcgctttg
    10081 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    10141 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    10201 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    10261 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    10321 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    10381 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    10441 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    10501 gcattccgca tctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    10561 gctggtgtgt taacgagatg gagcgtcgtt ataaactgat gtctgcgctg ggtgtgcgta
    10621 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    10681 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    10741 catacattgt ggtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    10801 tggaagagct gatagcacgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    10861 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    10921 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    10981 gcgcggaatc actgctgggg atgggggata tgctctactc tgggccgaac tccacgttgc
    11041 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    11101 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ttccgacagt gaaagcgaag
    11161 gtggcgcggg cggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    11221 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    11281 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    11341 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    11401 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    11461 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    11521 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca tcacctgtgc attactctca
    11581 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    11641 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    11701 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    11761 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    11821 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    11881 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    11941 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    12001 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    12061 tatcaactga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    12121 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    12181 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    12241 agcacagtat atcggccagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    12301 cgaagccggg catttacatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    12361 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    12421 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    12481 tcgccgcact attctttttg ttgacgaagt gcaccgattc aacaaaagcc agcaggatgc
    12541 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    12601 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtctatctgt tgaaatccct
    12661 gggtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaga cccgtggtta
    12721 tggtggtcag gatattgttc tgccagatga aacacgacgc gccattgctg aactggtgaa
    12781 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcaa
    12841 tgatagcggt aagcgggtcc tgaagcctga attattgacc gaaatcgccg gtgagcgaag
    12901 cgcccgcttt gataacaaag gcgatcgctt ttacgatctc atttccgcac tgcataagtc
    12961 ggtacgtggt agcgcacccg atgcggcgct ctactggtat gcgcgaatta ttaccgctgg
    13021 tggcgatccg ttatatgtcg cgcgtcgttg cctggcaatt gcttccgaag atgtcggcaa
    13081 tgccgaccca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    13141 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    13201 aagcaacgct gtctacactg cgtttaaagc tgcgctggcg gatgctcgcg aacgtccgga
    13261 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    13321 cgggcaggaa tatcgttacg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    13381 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    13441 caagattggc gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    13501 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    13561 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    13621 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    13681 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    13741 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    13801 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    13861 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    13921 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gtcgaagtca gccgctgggg
    13981 caccccgcgt gagtttgact ttgaagttcg tgaccatgtg acgctgggtg aaatgcactc
    14041 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    14101 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    14161 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    14221 gggtcagctg ccgaaatttg ctggcgatct gtttcatact cgtccgctgg aagaagaagc
    14281 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    14341 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    14401 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    14461 cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac tcaatggcgg cgctggaaga
    14521 gatgactggt catgcagaaa aagtcctgca gttgttgggc ctgccgtacc gtaaaatcat
    14581 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    14641 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    14701 gcgtcgtatg caggcacgct gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    14761 cctgaacggt tctggactgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    14821 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    14881 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctgcgggc gctttttttg
    14941 tctccctttg ataccgaaca ataattactc ctcacttaca cgtaatacta ctttcgagtg
    15001 aaaatctacc tatctctttg attttcaaat tattcgatgt atacaagcct atatagcgag
    15061 ctgctataga aataattaca caatacggtt tgttactgga atcaatcgtg agcaagcttg
    15121 agtgagccat tatgaaaacg aaaatccctg atgcggtatt ggctgctgag gtgagtcgcc
    15181 gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat ggccagcagc gcattaacat
    15241 taccttttag tcggattgcg cacgctgtcg atagcgccat tccaacaaaa tcagacgaaa
    15301 aggttatctg gagcgcctgt acagttaact gtggtagtcg ctgcccgcta cgtatgcacg
    15361 tcgtggacgg tgaaatcaaa tatgtcgaaa cggacaatac cggcgatgac aattacgacg
    15421 gcctgcacca ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcgccgt gtctacaatc
    15481 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    15541 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    15601 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    15661 cccgctcctg gccgccggga aataccctgg tcgcgcggct gatgaactgc tgcggcggct
    15721 atctgaacca ttacggcgac tactcctccg cgcaaattgc ggaaggtttg aactatacct
    15781 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaag ctggtagtgc
    15841 tgtttggtaa taaccctggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    15901 aacaggcacg ccagaaatct aatgcccgca tgatcatcat tgatccgcgc tataccgaca
    15961 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc gggaacagat gccgcactgg
    16021 ttaacggtct ggcgtacgtc atgatcactg aaaacctggt ggatcaggca ttcctcgata
    16081 aatattgcgt tggctacgat gagaaaaccc tgccagccag tgcgccgaaa aatggccact
    16141 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    16201 cacaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    16261 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    16321 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aacggaggca
    16381 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    16441 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    16501 aaatgacggc gctgcgtgat ggtgtacgcg ggaaagataa gctggatgtg ccgatcaaaa
    16561 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    16621 atgaaatcct tcaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    16681 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    16741 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    16801 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    16861 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    16921 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    16981 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    17041 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    17101 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    17161 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    17221 ttacgggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    17281 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcgcaaaaa cgcggtatca
    17341 acaacggcga taaagtcagg atctttaacg atcgtggtga agttcatatt gaggcgaaag
    17401 tgacgccacg aatgatgccg ggtgtggtcg cactgggtga aggtgcctgg tatgacccgg
    17461 atgcaaaacg tgtcgataag ggcggttgta ttaacgtact gaccactcaa cgtccgtctc
    17521 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    17581 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    17641 acctgcgagc tggcctgtaa agactacaaa gatttgacgc cagaagtcag cttccgccgc
    17701 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    17761 tttgcctact atctgtcgat ttcatgtaac cactgcgaag atccggcttg tactaaagtc
    17821 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    17881 tgcattggct gccgctactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    17941 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    18001 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggtcctat cgacgagctg
    18061 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    18121 ccgaatattg tgatcaaacc caatgccaac agccgcccga ccggggatac cactggctac
    18181 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    18241 cttcacggtc ttcgggcaat gtgtagcggg tggttttatc gttctggctt tggcgctgct
    18301 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    18361 ggtgctgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    18421 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    18481 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    18541 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    18601 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    18661 gatgggcttc ttcctgacga tgttcatggg cggcccgtta ctgggttacc tgttgctgag
    18721 tctggctggc gtcgatggct gggcgatgcg tctgctgcca gccatttctg tactggcact
    18781 ggtggtgagt ggtgtgatgt cagtgatgca gggcacagag ctggcaacta ttcatagttc
    18841 tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg ctgatgtcct ggcggatcgt
    18901 gcttttggcc gttgccctgt gcttgtggat tgcaccacag ctaaaaggtt atcagcctgc
    18961 ggttccgcta ctatccgtct cattcattct gcttctggca ggggaattaa tcggtcgcgg
    19021 cgtattctat ggtttgcata tgaccgtggg gatggccgtc gcaagctaat cagtaacaac
    19081 cggggtttcg gccccggttc tcttttattt ctgcttcgtt aacgtgtcat aactggtcat
    19141 caaattacga taatcaggaa tatggttaga gaacaacgtc gccagtcctt caatatcatt
    19201 acgccagtca cggtgcagct cgcaagccac gccgaaccat gtcatcaaat gcgcacctgc
    19261 ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca ttaaaggtcc ctgacgcgtc
    19321 agtcacaaca aagacatcaa acccttcttc aatggctgaa agcgccggga atgccacgca
    19381 aacttcggtt accacaccgg caataattaa ctgtttttta cctgtcgctt tgaccgcttt
    19441 tacaaaatct tcgttatccc aggcgttaat atttcccggg cgagcaatgt aaggtgcatc
    19501 ggggaattgt gctttcagtt ctggaactaa tgggccgtta ggaccggttt caaaactggt
    19561 ggtaagaata gttggtaagt tgaaatactt agccaggtca cccagcgcca gcacgttatt
    19621 tttaaactta tcgggttcga tatcccgtac aagggaaagt aaaccagctt ggtgatcaac
    19681 aagcaaaacg gcagcatcat ttttatcaag acgaacatac ggtttggtca tcctcttctc
    19741 ctttcgagag tcagtggtac gtctgaaatc ggtcagacgc gatgcattgc tctgaaagca
    19801 tagacgggaa atatgagttt gctgtgacca tgaaattttt cgactgaacg aagcgtatga
    19861 aatttgtgtt agatcaataa aaacaatcag atgagattaa tcgtctcttc agcattagcg
    19921 ttatgaatga gtaatataaa tcgataatac cgccgctgcg atatgcgcgt attttcaccc
    19981 attgacaatg tttttggcgg tggcatgatg cgcatgaaat ttgaacttcc tcacggtttt
    20041 aattcatgtc cacgtatacc cggcctgtca tgcttttgct gtctggcctg cttttgttga
    20101 ctctggcgat cgcggtgtta aatacactcg tgccgctttg gctcgcccag gaacacatgt
    20161 ccacatggca ggtaggcgtt gtcagctcat cctattttac cggcaacctt gtcggtacat
    20221 tgctgacagg gtatgtcatt aagcgcattg gctttaaccg cagctattat ctggcctcct
    20281 tcatttttgc cgctggctgt gccggccttg gcctgatgat tggattctgg agctggttgg
    20341 cttggcgttt tgtcgcgggc gtcggctgtg ccatgatttg ggtggttgtt gagagcgcgc
    20401 tgatgtgcag tgggacgtca cgtaaccgtg ggcgtttgct tgctgcgtat atgatggttt
    20461 attacgtggg aacgttttta ggccagttac tggtcagcaa agtttcaacc gagctgatgt
    20521 ccgtattgcc gtgggttaca ggtttgacgt tggcagggat cttaccgctg ttgtttacgc
    20581 gtgtgctgaa tcagcaggct gaaaaccatg attcgacgtc aattacgtca atgctaaaac
    20641 tccgtcaggc gcggcttggc gtgaatggct gcattatctc aggaatcgtt ctgggatctc
    20701 tatatggcct gatgccgctg tacctcaatc acaaaggggt gagcaatgcc agcattggtt
    20761 tctggatggc ggtactggtc agtgcgggta tccttggaca atggccgatt ggacgtctgg
    20821 cggataagtt tggtcgactg ctggtgttgc gtgttcaggt ctttgtcgtc attctcggca
    20881 gtatcgcgat gcttagccag gcggcgatgg ccccagcgtt attcatcctc ggtgccgctg
    20941 gctttacgct atatccggtg gcgatggcat gggcttgcga gaaagttgaa catcatcaac
    21001 tggtggcgat gaaccaggcc ttactgttga gttatactgt gggaagtctg cttggcccgt
    21061 catttaccgc tatgctaatg cagaatttct ccgataattt attgtttatc atgatcgcca
    21121 gcgtatcgtt tatctatttg ctgatgctgc tgcgcaacgc cggtcatacg ccgaaacccg
    21181 ttgctcacgt gtaaatgaat tcaagcagag tgtgaactta ctgtttcaca ctctgctttt
    21241 ttgtttcttc tatctgactt gctttattcc aaattttatt cgtttaaaaa taaaatgtgc
    21301 agcaggttat aattttgcat ttcgctattt ccgcacttct tatttgccgc gcataatccc
    21361 tcgttttaca gatgcccctt taattttggc gaaggatttg tctatggctg ggaatgttca
    21421 ggaaaaacag ttgcgatggt acaacattgc gctgatgtct tttatcactg tctggggttt
    21481 tggcaacgtt gttaataact atgccaacca ggggctggtg gttgtttttt catgggtgtt
    21541 tatctttgca ctctatttca caccttatgc gctaattgtt ggtcagttag gctcgacctt
    21601 caaagatggg aagggcgggg tcagtacctg gattaaacac acgatgggac ccggactggc
    21661 ttatctcgcc gcgtggacct actgggtggt gcatattccc tatctggcac aaaaacccca
    21721 ggcaattctg attgcgctcg gttgggcgat gaaaggcgac ggttcgctaa tcaaagaata
    21781 ttcagtcgta gcgttacagg ggttaacgct ggtgctgttt atcttcttta tgtgggttgc
    21841 ttcacgcggt atgaaatcgc tgaaaatcgt cggttctgtg gcagggattg cgatgtttgt
    21901 tatgtcgctc ctgtatgtgg cgatggcggt aaccgcgcct gcgattactg aagtgcatat
    21961 tgcgaccaca aacattacct gggaaacgtt cattcctcat atcgacttta cctacattac
    22021 cactatttca atgctggttt tcgcggttgg cggagcagag aagatttctc cttacgttaa
    22081 tcaaacgcgc aacccaggaa aagaatttcc aaaagggatg ctatgcctgg cggtgatggt
    22141 tgcggtttgt gccattctgg gctcactggc gatggggatg atgtttgact cgcgtaatat
    22201 cccggatgac ttaatgacta acggtcaata ttacgccttt cagaagctgg gcgagtatta
    22261 caacatgggt aatactttaa tggtgattta cgccattgcg aataccctgg gacaagtagc
    22321 tgcgctggta ttctcgattg atgccccgct taaagtgcta ttaggtgatg ctgacagcaa
    22381 atatattcca gccagtttat gtcgtaccaa cgcttctggt acgcccgtta atggctattt
    22441 tctgaccctg gtgctggtgg caattctgat tatgctgccg actctcggca tcggtgatat
    22501 gaacaatctc tataaatggc tgttgaatct taactcggta gtgatgccgc tgcgttattt
    22561 atgggtattt gttgcattta ttgcagtcgt tcgcctggcg cagaaatata aaccagaata
    22621 tgtctttatt cgtaataagc ctctggcaat gaccgtcggg atttggtgtt ttgcctttac
    22681 cgcctttgcc tgcttgacgg ggatcttccc gaaaatggaa gccttcactg cagagtggac
    22741 cttccagttg gcgctgaatg ttgcaacgcc gtttgtgctg gtaggattag gactgatatt
    22801 cccgctgctg gcgcgtaaag cgaatagtaa ataattattg tggtcggtga cgctgactct
    22861 ggcagatctg acccgttttt acataatcga gaaatgcgcg caagccgcag gacatatact
    22921 ggcggtttgg ataatagatc tggaagcctg aacgctctgt gctccagtct tccagtacac
    22981 attctaaacg accagtatca aggtactcct tgatctgttc atagagcaga tacccgatac
    23041 cagcacccat caaaacggcc tccagttcag catcaacatc atccagaatg atatttcctg
    23101 cgacagcaat ttccagctcc tttgcaaatt gccagtggaa aggtttgccg ctgggataac
    23161 gaaaaacaac acattgatga ttaagcagat catgaggatg gcggggtttg ccatatcttg
    23221 caaagtactc tggcgttgct gctacacata atttcactgg tgggccgatc gccacgctga
    23281 tcatatcttt ttcgacgatg ccactaagtc gaacgccagc gtcaaagccc tgttggacga
    23341 tatcgacaag agaatcgtca gttgtgagct cgactttaat atcaggatat tcacgtgtga
    23401 aaccaaccag taaaggcatt aaaaatatac gtgcagctac ccgggcggca ttgattttga
    23461 gcgttcctgt cggtgtcaga cgaaaatcat tcatctcatc taacatgatc tgaatttcgt
    23521 cgaaagctgg gcgcaatcgt tcatagagat tagaacccgc ttccgttaac gatacgcttc
    23581 gggttgtccg attgaataag cgaattttaa gacgttgttc cagtgtttta atactatggc
    23641 taatggcgga cgaggataag cctaattcat cgcccgctgc acgaaagctt tgattacggg
    23701 ccacggcaaa gaaagtggca aagtcagaca tattcatccg cattagtgaa aatccttcat
    23761 taactcatcc tgaatcatca cgtttacagg attatgccgc ctgcgtaaag tgcgctccag
    23821 aacttaacgt ggaggtaaaa ttatgcagtc tgaacgtatt tatttggtat gggcccatcc
    23881 tcgtcatgat tcattgaccg cacatattgc tgatgcgatc catcagcggg caatggagcg
    23941 gaaaatacag gtgacggaac tcgatttata tcggcgtaat ttcaacccgg tgatgacgtc
    24001 ggaagatgaa ccagactgga agaatatgga taaacgttat tctccagagg ttcatcagct
    24061 ttattcagag ctgcttgaac atgacacgtt agtggtggtt tttcctttct ggtggtacag
    24121 cttcccggca atgctaaaag gatatattga cagagtatgg aataatgggc tggcttatgg
    24181 agatgggcac aaattaccat tcaataaagt tcgttgggtg gcgctggttg gaggagacaa
    24241 agaatcattt gtccagatgg gctgggaaaa aaatataagc gattatttga aaaatatgtg
    24301 cagttatctt ggtattgaag atgccgatgt cactttcttg tgtaatacag tggtattcga
    24361 tgggcaagaa cttcatgcga gctattatca gtcgttatta tctcaggtac gggatatggt
    24421 agatgcacta taaaatgtgt taaaaacgct gtagcagaat gaagcgcgga ataaaaaagc
    24481 ggcaactcaa taaagttgcc gctttacggg gaaattagaa cattacctta tgaccgtact
    24541 gctcaagaat gcctttcacg cgttccatgg tctctttctt aggtggttta acaccgtcga
    24601 gtttgtactc ttcacccatt gccacccatt tgtgtttgcc cagctcgtgg taggggagga
    24661 gttcgatttt ctcaacgttg cccatatcac gggtaaattc accgaggcga tgcgctgaat
    24721 cgtcatcgtc agaccagcct gggacaacaa cgtagcggat ccacaccttc acatttttgt
    24781 tcgccagata tttggcgaac tccagcgtgc ggtggttgga aactccaacc agattttggt
    24841 ggatctcgtc gttcatctgt ttgagatcga gcattaccag gtcggttact tccagcagtt
    24901 catcaatcac cggatcgtaa cgacgaacaa aaccgttggt gtccagacag gtatgaatgc
    24961 cttctttttt gcaggcgcgg aaccagtcac gaacaaactc agcttgtagg attgcctcac
    25021 cgccggatgc ggtaacgccg ccgccggaag cgttcataaa gtggcgatag gtcaccacct
    25081 ctttcattaa atcttcaacg gtaacttctt taccgccatg cgtatcccag gtgtcgcggt
    25141 tatgacaata caggcagcgc atcaggcagc cctggaaaaa ggtgataaag cgaatacctg
    25201 ggccgtctac ggttccacag gattcaaagg agtgaatgcg accaataact gacattgcgg
    25261 tgtttctcca gatgtggccc atctgaggcc gtgttggtgc gcagctcgaa ggctacgtcg
    25321 agtctgtttt ggcagtcacc ttaaagtata gatagctgac aaaaaaggct ctcgtgctaa
    25381 aaaaggcccc actttcgtgg agcctttatt gtacgctttt tactgtacga tttcagtcaa
    25441 atctaattac atagattgag tgaaggtacg agtaataacg tcctgctgct gttctttagt
    25501 cagcgagttg aaacgtactg cgtagccaga tacacggatg gtcagctgcg gatatttttc
    25561 cgggttttcc atcgcgtcga gcagcatttc acggttcatc acgttaacgt tcaggtgctg
    25621 accaccttcg atggatgctt cgtggtggaa gtaaccatcc atcagaccag ccaggttggt
    25681 cttacgaact tcgtcgtctt tacccagtgc gttcggaacg atagagaagg tgtaggagat
    25741 accatcttta gcgtaagcaa acggcagttt agcaacggaa gtcagagacg ctacagcacc
    25801 tttctggtca cgaccgtgca tcgggttagc acccggtccg aacggcgcgc cagcacgacg
    25861 accgtctggg gtgttaccag ttttcttacc atacacaacg ttagaagtga tggtcagaac
    25921 agactgagtc gggatagcgt cacggtaggt gtgcagtttc tgaattttct tcatgaaacg
    25981 ttctaccagg tcaacagcca ggtcatctac acgcggatca ttgttaccaa actgcgggta
    26041 ttcgccttcg atttcgaagt cgatagccag accgtcttcg tcacgaatcg gtttaacttt
    26101 cgcatatttg attgcagaca gggagtcagc agcaacggac agaccagcga taccacacgc
    26161 catggtgcgg ataacgtcac ggtcgtgcag cgccatcaga gaggcttcgt agctgtactt
    26221 gtcgtgcatg tagtggatga tgttcagtgc agtgatgtac tgtttagcca gccagtccat
    26281 gaagtgatcc atgcgctcca tcacttcatc atagttcagg acatcgcctt tgatcggttc
    26341 agacttcgga ccaacctgca ttttcagttt ttcgtcaacg ccgccgttga ttgcgtacag
    26401 catggttttc gccaggtttg cacgcgcacc gaagaactgc atttgtttac caacgatcat
    26461 cgggcttacg cagcaagcaa tagcgtagtc atcgttgttg aagtccggac gcatcaggtc
    26521 atcgttctca tactgcagag aagaggtgtc gatggacact ttagcggcga atttcttgaa
    26581 gttcagcggc agtttttcag accacagaat ggtcatgttc ggttccggag acggacccat
    26641 ggtgtacagg gtgttcagga aacggaagct gtttttggta accagggtac gaccgtcgag
    26701 gcccatacca ccgatagatt cggttgccca gatcgggtcg ccagagaaca gttcatcgta
    26761 ttccggagta cgcaggaagc gaaccatacg cagtttcatg accaggtggt caaccatttc
    26821 ctgcgcttct tgttcggtga tcttgccagc tttcaggtca cgttcgatgt acacatccag
    26881 gaaggtggag gtacgaccga aggacattgc agcaccgttc tgagacttaa cagcagccag
    26941 gtagccgaag taagtccact ggatagcttc ctgagcgttg gtagccggac cagagatgtc
    27001 gtagccgtat ttcgcagcca tttctttcat ctgacccaga gcgcggtgct gttcagcgat
    27061 ttcttcgcgc agacggatag tctgttccag gtttacgccg ttttccagat cagcctgcag
    27121 agaagtgaac tgtgccagtt tgtctttcat caggtagtcg ataccgtaca gcgcaacgcg
    27181 acggtagtca ccgatgatac ggccacggcc atatgcatct ggcagaccgg tcagaacacc
    27241 agatttacgg caacgcagga tgtccggagt gtaaacgtcg aacacgccct ggttgtgagt
    27301 tttacggtat tcagtgaaga tttttttgat catcggatcc agttcgcggt tgtacgcttt
    27361 gcaggaacct tcgatcattt tgataccacc gaacgggata agagcacgtt tcagcggagc
    27421 ttcagtctgc agaccaacga ttttctcaag ctgcttgttg atgtagccag cgtcgtgaga
    27481 ggtgatggtg gaagcaacag cggtgtcaaa gtcaactggc gcgtgagtgc ggttttccag
    27541 tttaacgcct tccattactt tgtcccacag ggtggtggtc gcttcagtag cgccagccag
    27601 gaaggactcg tcaccctcgt acggagtgta gtttttctga atgaagtcac ggacgtttac
    27661 ttcattctgc cagtcacctt tggtaaaacc ttcccaggct gtggctaact tttcattaag
    27721 ctcggacatg taacacctac cttcttaagt ggatttttta tttactgcgt acttcgacaa
    27781 ccattaatgg tgatcgtttt cacgcaggta aatgacccag tatgtcaacc caaccaacaa
    27841 accaccgccg ataatattac cgatcgtaac cggaatcagg ttatcagtga tgaagttcat
    27901 cacggtcagg tgagaaaaat tttccggtgc agaaccgaca gcggtccaga attccggaga
    27961 tgcgaagtcg cggattacaa tacccatcgg gatcataaac atgtttgcga tactgtgctc
    28021 aaaaccgctg gcaacaaaca tcgcgaccgg cagcaccata atgaacgctt tgtccatcag
    28081 gctacggcca gaataactca tccatactgc cagacatacc atcaggtttg ccaggatacc
    28141 aaggcagacg gcctcaataa aagtatggtg cactttgtgg tcggcggttt gtaggacgtt
    28201 tagtccccat tgaccatttg cggtcatata ctcgccggaa agccacatta aaagtacaaa
    28261 cagcagtgcg ccgaccaggt tgccaaaata gacatttagc cagtttttcg ccaactgacc
    28321 ccaggtgatg cgcccactcg ccttagcaac aacaatcaac actgtggaag taaagagatc
    28381 ggctccgcag acaacacaaa gaatcagccc cagagagaag caaatgccgc caaccagttt
    28441 tgccatgccg aagggcattg tgcctgtgcc agtggttgct gtgatataga agacgaatgc
    28501 gattgagatg aaaacaccgg cggtaatcgc cagatagaaa gtcttaagcg gatgtttcgt
    28561 tgctttatag acacccgcct cttcggccac tttggccatt gcagcaggaa gtaaaagatc
    28621 aaaagggttg tcagctttca cactaactct ctctttatta agtcggcgac gagatactaa
    28681 caaagcatta tagatgagaa attgatatag atcatatctc gcctggctta taggcccgta
    28741 actcgcatgg tttttatgca aatacggagt aaatatttga ttatccaaat aaaaataaat
    28801 tttaaaaatt aacaaatgag ttgaattttt tccg
//
LOCUS       DAZFVO020000052        28657 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFVO020000052 DAZFVO020000000
VERSION     DAZFVO020000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.052"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHKNB_RS10500"
                     /old_locus_tag="pgaptmp_002100"
     CDS             complement(<1..242)
                     /locus_tag="ACHKNB_RS10500"
                     /old_locus_tag="pgaptmp_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4195604.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKNB_RS10505"
                     /old_locus_tag="pgaptmp_002101"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKNB_RS10505"
                     /old_locus_tag="pgaptmp_002101"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HGU4195605.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHKNB_RS10510"
                     /old_locus_tag="pgaptmp_002102"
     CDS             2255..3904
                     /locus_tag="ACHKNB_RS10510"
                     /old_locus_tag="pgaptmp_002102"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4195606.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGVYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3985..4557)
                     /locus_tag="ACHKNB_RS10515"
                     /old_locus_tag="pgaptmp_002103"
     CDS             complement(3985..4557)
                     /locus_tag="ACHKNB_RS10515"
                     /old_locus_tag="pgaptmp_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGU4195607.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRT"
     gene            5077..5976
                     /locus_tag="ACHKNB_RS10520"
                     /old_locus_tag="pgaptmp_002104"
     CDS             5077..5976
                     /locus_tag="ACHKNB_RS10520"
                     /old_locus_tag="pgaptmp_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4195608.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKNB_RS10525"
                     /old_locus_tag="pgaptmp_002105"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKNB_RS10525"
                     /old_locus_tag="pgaptmp_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HGU4195609.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="ACHKNB_RS10530"
                     /old_locus_tag="pgaptmp_002106"
     CDS             7449..8771
                     /locus_tag="ACHKNB_RS10530"
                     /old_locus_tag="pgaptmp_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195610.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9005..11065)
                     /locus_tag="ACHKNB_RS10535"
                     /old_locus_tag="pgaptmp_002107"
     CDS             complement(9005..11065)
                     /locus_tag="ACHKNB_RS10535"
                     /old_locus_tag="pgaptmp_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HGU4195611.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHKNB_RS10540"
                     /old_locus_tag="pgaptmp_002108"
     CDS             complement(11135..11902)
                     /gene="pdeH"
                     /locus_tag="ACHKNB_RS10540"
                     /old_locus_tag="pgaptmp_002108"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HGU4195612.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHKNB_RS10545"
                     /old_locus_tag="pgaptmp_002109"
     CDS             12134..13063
                     /gene="kdgK"
                     /locus_tag="ACHKNB_RS10545"
                     /old_locus_tag="pgaptmp_002109"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HGU4195613.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13159..14655)
                     /locus_tag="ACHKNB_RS10550"
                     /old_locus_tag="pgaptmp_002110"
     CDS             complement(13159..14655)
                     /locus_tag="ACHKNB_RS10550"
                     /old_locus_tag="pgaptmp_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4195614.1"
                     /translation="MQGTKIQLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLADSTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHKNB_RS10555"
                     /old_locus_tag="pgaptmp_002111"
     CDS             complement(14876..16162)
                     /gene="dctA"
                     /locus_tag="ACHKNB_RS10555"
                     /old_locus_tag="pgaptmp_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HGU4195615.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHKNB_RS10560"
                     /old_locus_tag="pgaptmp_002112"
     CDS             complement(16345..18333)
                     /gene="hmsP"
                     /locus_tag="ACHKNB_RS10560"
                     /old_locus_tag="pgaptmp_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HGU4195616.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHKNB_RS10565"
                     /old_locus_tag="pgaptmp_002113"
     CDS             complement(18415..21888)
                     /gene="bcsC"
                     /locus_tag="ACHKNB_RS10565"
                     /old_locus_tag="pgaptmp_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HGU4195617.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHKNB_RS10570"
                     /old_locus_tag="pgaptmp_002114"
     CDS             complement(21870..22976)
                     /gene="bcsZ"
                     /locus_tag="ACHKNB_RS10570"
                     /old_locus_tag="pgaptmp_002114"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HGU4195618.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHKNB_RS10575"
                     /old_locus_tag="pgaptmp_002115"
     CDS             complement(22983..25322)
                     /gene="bcsB"
                     /locus_tag="ACHKNB_RS10575"
                     /old_locus_tag="pgaptmp_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HGU4195619.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="ACHKNB_RS10580"
                     /old_locus_tag="pgaptmp_002116"
     CDS             complement(25333..27951)
                     /gene="bcsA"
                     /locus_tag="ACHKNB_RS10580"
                     /old_locus_tag="pgaptmp_002116"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HGU4195620.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27948..>28657)
                     /gene="bcsQ"
                     /locus_tag="ACHKNB_RS10585"
                     /old_locus_tag="pgaptmp_002117"
     CDS             complement(27948..>28657)
                     /gene="bcsQ"
                     /locus_tag="ACHKNB_RS10585"
                     /old_locus_tag="pgaptmp_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HGU4195621.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
BASE COUNT         6757 a         7907 c         7239 g         6754 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagtgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg cgtctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctaggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgga taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5401 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5461 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taaacagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gtgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt gtcgatgatc ccgattttga tggtatcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcac tttattccgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6601 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttc agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggctgatgcc tttggtcgcc
     8401 gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cgctgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatgggact gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccgacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8941 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     9001 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     9061 gtgagatcag tgccagtaac gccgccgccg gtgtaaccag cgttgccagt gccgcagcaa
     9121 cagcaccacg ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc
     9181 cacgcacata cagcggcgaa cgcagtgtga taatccgaat tcctttactc tccggatcaa
     9241 tagtcaaatc cagctgttcg gaagcaaaac ttgccgtgcc ggtaacgtta atcaacgcgt
     9301 tctcagtatc gaaagcaaag atctgcgggc gcgccacgcc gttggcaata tccagattcg
     9361 ccgccgcgca gttcacccgc acctcatcat caccaaatat cgcaccgaca atgtagttgc
     9421 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9481 gtttcaggtt gccgttactg ttgcctaaaa gtgccgccac cgagttaccg ctaccgcgca
     9541 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9601 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9661 cgccttccag atgaatattc gccgcaatgc tgccacccgc catgccaaat ttcagcggtt
     9721 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9781 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9841 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9901 ccttcttctg ttcagaccgt ttcgactttt cagccccttt cccggaatca acgccaatca
     9961 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
    10021 gtggcttgcc ggtggtgtag atcagagaac cgtggatatc gctatcgcca atgcgcccat
    10081 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
    10141 catctgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
    10201 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
    10261 tcacgacgcc atcaaaagca acacgggtat tacctgaacg gaaatcagcc tgtaccggaa
    10321 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta ccagtgagcg
    10381 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10441 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggctta cctaacggat
    10501 cgacaaaaat ttccagatcc gctttgctca ctttgtcatc aatggcgatc cgcccttgat
    10561 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10621 tatcatcgtt ggcgagatta aacgtccagt tattgttctt ttcagagagg cgaatcaggc
    10681 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10741 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10801 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccatggcacc cagctgcgcc
    10861 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10921 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10981 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagcccct gaaatcgcag
    11041 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    11101 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    11161 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    11221 tctgaacatc acgccactct tccggtgttt ctacgccctc gacaatcacc ccgcgacaat
    11281 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg gccttccggc gactgacgca
    11341 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag
    11401 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11461 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11521 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11581 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11641 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11701 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11761 ccacggccat taaccgcccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11821 actgcaacca aaaacgccgt tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11881 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11941 tcgacaaaga ttatcggcgc gcaagggcag aactttaccg cctggccggg gtaaatttcc
    12001 cctctccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    12061 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    12121 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    12181 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat acactgaata cttccgtcta
    12241 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    12301 ggacagtttt agccagcaga tgctggacgc ctggcacggc gagaacgttg atacttccct
    12361 gacccaacgg atggaaaacc gtcttccggg cctttactac attgaaaccg acagcaccgg
    12421 cgaacgtacg ttctactact ggcgtaacga agccgccgcc aaattctggc tggagagcga
    12481 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12541 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12601 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12661 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12721 gacgctggac gatgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12781 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12841 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12901 gatcgatacc accgcagctg gcgactcttt cagtgccggt tatctggcgg tacgtttgac
    12961 gggcggcagt gcggaaaacg cagcgaaacg cgggcacctg accgcaagta ccgttattca
    13021 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    13081 ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta
    13141 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    13201 atccgtggcg acagaggtgg cggcagcagc agtcgatggg gccatgattt gatcccaggc
    13261 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    13321 catatcattc gacaactgct gacgcagatc ctgatttaac atctccaccg tcaggctgtt
    13381 aaggaaatcc tgccgcagtt tctgatactg ctccggcgcg atatcgacga cctgattttg
    13441 caacgaacgc atccgctgac ccatcagaat atcggtatca gcccgcgcat aggcggcaaa
    13501 cagtttctgc aactccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13561 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13621 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13681 aaatccaaga ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa
    13741 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13801 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13861 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13921 tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc gcgcatccac
    13981 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    14041 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    14101 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgccac
    14161 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    14221 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    14281 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    14341 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    14401 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactatcgg cgagcgaacc
    14461 ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14521 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14581 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagttgaat
    14641 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14701 gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14761 tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc
    14821 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag
    14881 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14941 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    15001 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    15061 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    15121 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    15181 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    15241 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    15301 atttgcggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    15361 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    15421 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15481 tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15541 tggtaaacgc cattgccccg aacgcaccaa taggcgccag acgcatgatc atattgatga
    15601 tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc
    15661 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15721 cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15781 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15841 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15901 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15961 tcatgctttc catgcccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    16021 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    16081 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    16141 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    16201 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    16261 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    16321 agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc
    16381 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    16441 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16501 cgccagcatg ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat
    16561 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16621 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16681 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16741 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16801 tgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16861 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16921 cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16981 ttgctggata cgcagtaaca cttccgcact gaccagtttt ccgctggtca tctcgacctg
    17041 tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    17101 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    17161 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    17221 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    17281 aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga ttgcgtgcca
    17341 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    17401 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    17461 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17521 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17581 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17641 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17701 cagacgcggt aatgccagtt ggtgaccaac aagctcctgg gctgggatgg cgttaagttc
    17761 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17821 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17881 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17941 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    18001 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    18061 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    18121 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    18181 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgttgga ccagatggaa
    18241 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    18301 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    18361 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    18421 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18481 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18541 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18601 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18661 gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc gtgaatgcga
    18721 ccacgagcca gacgccccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18781 cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18841 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18901 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18961 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    19021 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    19081 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    19141 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    19201 gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc agctccagac
    19261 gtcatttcgc cagccgaccg ccacgctggc accggaatcc gactggctgc ggttgccgct
    19321 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    19381 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    19441 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19501 gctcgaaccc cagtaatcgt gctcaagggt gacgttaaga tcctgctggc gatagaggtc
    19561 cgccgcatcg ctgcgcacgc cgcgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19621 tcgggtgaat gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19681 gtctttgtag gtttccagcg cctgctttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19741 gtcgcgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19801 cttattaaac gtccgctgcg ctgctgcggt atcgccaagc tgcgcctgtg ccagcgccac
    19861 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19921 tgccgccgct ttgtcaccgg cagcaatatc cacttccgtc agaccaagaa tggcgtcggc
    19981 gttagctggc tcccgcgtca ggacattctg atatgcagca cgggcggcgg tgtaatcacg
    20041 tcgttgttgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctgacg
    20101 cagcatcgct tccgcttcag cttctttgcc gctttctcgc aggcgattag cggtttccag
    20161 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcgcg
    20221 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agaggtacag
    20281 cccgtaagcg taaacctgct ccgggtcgtt cggcttatgc tgcgccagat tgcgcattaa
    20341 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    20401 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    20461 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20521 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20581 tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt tagtgttgcc
    20641 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20701 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20761 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20821 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20881 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20941 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    21001 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    21061 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    21121 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    21181 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    21241 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    21301 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    21361 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    21421 caccgtactc cagtactcga cggcaatgtc accttccggc ggcgcaccgt tgaacagttt
    21481 gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc gtgcctgttg
    21541 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21601 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21661 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21721 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21781 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21841 ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21901 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21961 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    22021 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    22081 ccgaccggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    22141 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttttgc
    22201 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc gtagctgctg
    22261 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg cacccagtct
    22321 ggcgaaaaac ctttcggggc ggtttccagc aataaacgtt gattggtttc gcgcagcgtg
    22381 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    22441 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22501 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22561 tcggtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22621 acatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22681 ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt gttctgcgtc
    22741 cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaaa gaacatgccg
    22801 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22861 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22921 acactaaagg cagccagcag cagcatcgtc acgattccac tacgcaacac attcatcttc
    22981 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    23041 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    23101 gccagcgcat accacacgcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    23161 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    23221 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    23281 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    23341 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    23401 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc
    23461 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23521 atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23581 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23641 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23701 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23761 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23821 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23881 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23941 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcaggttg
    24001 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    24061 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    24121 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    24181 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tacccagttc
    24241 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    24301 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacag gtctttgtcg
    24361 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    24421 atcacagggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24481 gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa gttctgccca
    24541 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24601 acatccggcg cacccgcaaa gaccatcggc aaggtgttag tacggttatc gcgcgggtca
    24661 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24721 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24781 acgtcctgat aatggccgac aaactccagc cgcacacggt tgaagtcggt aataaacagt
    24841 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24901 acgcccatca gctcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24961 ggcgatggcg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcata
    25021 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggc
    25081 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    25141 gcatcagcgc cctgcacgcc cggcatcact tgccccacct gcggattttg ttccgtctgg
    25201 gcggctacag ctggctcagc attgatcagt ggttgcgttg ctggcgtcgc ctgcgtcatg
    25261 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    25321 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    25381 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    25441 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25501 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25561 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25621 gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct gggtcgggaa
    25681 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25741 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25801 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25861 tcggcgtacc tgtttgcttt ctaccgatac cgcaactgcg ccgccaagaa caatcaggtt
    25921 gtagaacacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25981 gtagcgccag atgcctaccg caacgcccac caggttgagt agaacaagga agatgtaggg
    26041 ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    26101 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    26161 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttcga
    26221 gttggtcagg ctggcatgga tcatatgcgg cagcacgaac agggcgatca tcaacgctgg
    26281 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    26341 tcgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgggcgaa
    26401 tttcagccct ttaccggtaa gcgggttatc gagacggaag atttgcacca tcccgcgtgc
    26461 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26521 cggaatacgc atatatgcag aggtgtagcc acgacggtgc aggcgcagag acgtgtgcgc
    26581 atcttcggtt accgtttcga cagcaatacc gccaatttca tccaatggtt tgcgacgaat
    26641 tactgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26701 accatagaac agcgtgcctt cgttcggtgt tttacgaaaa cgccccaggt tgcgttcaaa
    26761 cgggtccggc gagaagaagt ggtgtggagt ctgcatcatc gccagctgtt tctctttcag
    26821 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26881 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26941 ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    27001 ttccctgccg ccgtcatcga ggatccagat gttcagtttg tctttcggcc agtcgatacc
    27061 cagcgaggcg taaatggtat ttttcaccac gttgagatct tcgttgtaag tcgggacaaa
    27121 gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct gacgattcag
    27181 cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg tttcagcgaa
    27241 gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg tagaggtgta
    27301 acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca ttagcgccga
    27361 gaagcgcccc ggcatccgcc gtacgatcag cgctaccccc cacagcagca tcaggaagat
    27421 aaactgcgcc agcgggttaa acggctgagt aacgcagatt aacgccagaa tcagcgagaa
    27481 ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac cgagctcttt
    27541 tttctcatcc agatgctgtg ttttatggct aacgcgctca ggcagctcgt tcatccattg
    27601 atggtaacgt ccacgaatat tttgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt
    27661 ttctttgcgc gatgcaccga tcaataacca gcatgtttga atgagataac ggaccgggtc
    27721 cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt ctgcgcgaat
    27781 acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca tccagaaaca
    27841 gccgagcgtc gcgctgaaag ccgacgcacc gtgacgacga taatcgcgat aacgcccgat
    27901 aagccgcgcg ttgaccggcg ggataagcaa ccaccgggtc aggatactca tgatgcactc
    27961 ccgactggcg ttttcagccc ggagtagttc aacaggcacc agttcgccag cgtcagtatc
    28021 tcttcagccg ccagcgcatc actgcgatat tcacctactg gttgcttagc cgccaggcat
    28081 tcagccatcg cttcatcacg atgaatgagc atcggcagta atcggcgctg gctttgcaac
    28141 caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagtcatt aatcaaaata
    28201 tgtgcgccat ccggcagcgc ttgctgatgc agtcggatat ggcagttggc atccacattg
    28261 acgattgcca gcgagtgatc gcacaaactc agcagctggt gggttatctg cgaggcatca
    28321 cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg ctgtaagccg
    28381 gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc ttcaatggat
    28441 aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccagc gtcacgccag
    28501 tcctggccat ccagcatcgc tctggcccag ccctgacggt gggtaaaatc aacattaaat
    28561 gacaggcgca acaagttgtc cgggcaggca tcgaccacca gaacattttc tcccaacatt
    28621 tgtaatgacc aggctaatgc ggcggtgatg gttgttg
//
LOCUS       DAZFVO020000053        28638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFVO020000053 DAZFVO020000000
VERSION     DAZFVO020000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.053"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            51..1217
                     /locus_tag="ACHKNB_RS10590"
                     /old_locus_tag="pgaptmp_002118"
     CDS             51..1217
                     /locus_tag="ACHKNB_RS10590"
                     /old_locus_tag="pgaptmp_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4195622.1"
                     /translation="MFQKKTRKMSLSRALDKYLKTVSVHKKGHLQEFYRVNVIKRHPM
                     ADRYMDEITTIDIAGYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGTC
                     RMNPVELVRKPKISNGRDRRLTSGEERRLSRYFRDKNPQLYVIFHLALETAMRQGEIL
                     SLRWEHLDLQHGVAHLPETKNGSPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFKS
                     AWRTALLDLKIENLHFHDLRHEAISRLFELGTLNVMEIAAISGHRSLNMLKRYTHLRA
                     YHLVSKLDARRKQTSKISPYFVPYPATVGCRNGLFVVTLHDFDLETRAETRELAISHA
                     SVLLLRALAQAAQRGERVPTPGELPANIDERVMICPLTNSQFMTKANSIFISSS"
     gene            1259..1549
                     /locus_tag="ACHKNB_RS10595"
                     /old_locus_tag="pgaptmp_002119"
     CDS             1259..1549
                     /locus_tag="ACHKNB_RS10595"
                     /old_locus_tag="pgaptmp_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195623.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(1979..2314)
                     /locus_tag="ACHKNB_RS10600"
                     /old_locus_tag="pgaptmp_002120"
     CDS             complement(1979..2314)
                     /locus_tag="ACHKNB_RS10600"
                     /old_locus_tag="pgaptmp_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195624.1"
                     /translation="MKIKQHKEKLEIIRNIIRETLLGNAAIVVLVSMIFVMLEKPWSV
                     MSVLCIFVYCISLMIVSLWWFFIVFIYISELTESVNIMRVLVAIWVLSSESFIIYNII
                     GVFHKVFMY"
     gene            2576..3397
                     /locus_tag="ACHKNB_RS10605"
                     /old_locus_tag="pgaptmp_002121"
     CDS             2576..3397
                     /locus_tag="ACHKNB_RS10605"
                     /old_locus_tag="pgaptmp_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HGU4195625.1"
                     /translation="MKPDSTGIATRMMLSLDRERICECLLSNRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEEALSEMFLPADNIVRRLLCRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGDILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PVTQEAFCRIISSGENVDIILWHGFPFAGTHSGEYCGVLLRQKKEINNAIIYPVLSLY
                     PVRMDMEKEGWFQMVRKISPDIPVRAVTLTPAQAEYLITGKILPTVLFRVSLSPW"
     gene            3501..4703
                     /locus_tag="ACHKNB_RS10610"
                     /old_locus_tag="pgaptmp_002122"
     CDS             3501..4703
                     /locus_tag="ACHKNB_RS10610"
                     /old_locus_tag="pgaptmp_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HGU4195626.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            4843..5013
                     /locus_tag="ACHKNB_RS10615"
                     /old_locus_tag="pgaptmp_002123"
     CDS             4843..5013
                     /locus_tag="ACHKNB_RS10615"
                     /old_locus_tag="pgaptmp_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195627.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            5062..5514
                     /locus_tag="ACHKNB_RS10620"
                     /old_locus_tag="pgaptmp_002124"
     CDS             5062..5514
                     /locus_tag="ACHKNB_RS10620"
                     /old_locus_tag="pgaptmp_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HGU4195628.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            5511..6347
                     /locus_tag="ACHKNB_RS10625"
                     /old_locus_tag="pgaptmp_002125"
     CDS             5511..6347
                     /locus_tag="ACHKNB_RS10625"
                     /old_locus_tag="pgaptmp_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HGU4195629.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQIQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            6331..7479
                     /gene="traJ"
                     /locus_tag="ACHKNB_RS10630"
                     /old_locus_tag="pgaptmp_002126"
     CDS             6331..7479
                     /gene="traJ"
                     /locus_tag="ACHKNB_RS10630"
                     /old_locus_tag="pgaptmp_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HGU4195630.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTVQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            7476..7766
                     /locus_tag="ACHKNB_RS10635"
                     /old_locus_tag="pgaptmp_002127"
     CDS             7476..7766
                     /locus_tag="ACHKNB_RS10635"
                     /old_locus_tag="pgaptmp_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGU4195631.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            7830..11861
                     /locus_tag="ACHKNB_RS10640"
                     /old_locus_tag="pgaptmp_002128"
     CDS             7830..11861
                     /locus_tag="ACHKNB_RS10640"
                     /old_locus_tag="pgaptmp_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HGU4195632.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAE
                     AGTDLSLLKRWLPRPQEMSMDAGDPVTEFRQVLENAGLVLEKDPEMDGEIHRVPTRDD
                     RRGSQSGAYKAYLDGRPSGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNR
                     DEKERKLQQQYNKQAGYARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHE
                     ATGHPVLMTIDADNMINVAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQHVMNAQLKHIGIPTPNSDIADHREA
                     VVIGNLIFTPVKDEKPQMSPENKQSTAPETVLATPDTPYDIHTEETENQAYEDDYAAY
                     ASLQSVTTETLSPDKPLENIASAADTGTENTSAATAGDQEDLNTDATGTDTAHNTNTA
                     ETGTLPEPAEIQPVQKSSPEPTAEDKPQESVIPAADTGEKNASATTADNQSVQAEELS
                     PGDDTPETSVSDATGTQHTTTTQTEEQLQVSMASADDWSAFAEDLNADATVMTTAHHT
                     DTTETASPSEPAGTQPVQQATPEATAENKPQEEDVLSATDTSEKSASAVPDNNRDAPP
                     EELNADATVAATVHNTGTAETASPSEPAETQPVRQATAEPTAEDKPQEERLVSATETS
                     TANEAARPETPSLFSRLRRFLTGGKSRTSEPSVIQEKKPTEAETATETAAPPSPPETS
                     PEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLEHKEFPDRTALYSVDGEPAFLDRL
                     YCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKL
                     PAQRAALEKLRKEKEMVHDAIVTHRPTPELNRQSPEETTMAQPEPPMSPKAEAEQTAG
                     CTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLS
                     PGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGKGLEELVKNHQKGDPVTL
                     TLQARERSPSSPGKPEWVRNIWTMKPVCNGISVSHDNPHEGQYIREYPAATFRQMMTL
                     LQQGWPQLMQDVRLPDKVPDTLYLGEDRQPAAAPRDPALRVPLNGTPPATLTPLIFSV
                     DKQSQRLDLLLLQSADEYMQGVVRLNGTLYPALATPTADSRQLVINAMTDRGLQFAGY
                     GEAINRDADGPKRPAPQLMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWS
                     DSQKAEDRQEKTLQQAQSHSPGL"
     gene            11878..12228
                     /locus_tag="ACHKNB_RS10645"
                     /old_locus_tag="pgaptmp_002129"
     CDS             11878..12228
                     /locus_tag="ACHKNB_RS10645"
                     /old_locus_tag="pgaptmp_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HGU4195633.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            12240..12935
                     /locus_tag="ACHKNB_RS10650"
                     /old_locus_tag="pgaptmp_002130"
     CDS             12240..12935
                     /locus_tag="ACHKNB_RS10650"
                     /old_locus_tag="pgaptmp_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HGU4195634.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            12946..13920
                     /locus_tag="ACHKNB_RS10655"
                     /old_locus_tag="pgaptmp_002131"
     CDS             12946..13920
                     /locus_tag="ACHKNB_RS10655"
                     /old_locus_tag="pgaptmp_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HGU4195635.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQAPGNA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            13924..15255
                     /gene="traO"
                     /locus_tag="ACHKNB_RS10660"
                     /old_locus_tag="pgaptmp_002132"
     CDS             13924..15255
                     /gene="traO"
                     /locus_tag="ACHKNB_RS10660"
                     /old_locus_tag="pgaptmp_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HGU4195636.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAAIRKQNEMRQRYGIQQESHP"
     gene            15252..15965
                     /gene="traP"
                     /locus_tag="ACHKNB_RS10665"
                     /old_locus_tag="pgaptmp_002133"
     CDS             15252..15965
                     /gene="traP"
                     /locus_tag="ACHKNB_RS10665"
                     /old_locus_tag="pgaptmp_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HGU4195637.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     VAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVSPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            15962..16492
                     /gene="traQ"
                     /locus_tag="ACHKNB_RS10670"
                     /old_locus_tag="pgaptmp_002134"
     CDS             15962..16492
                     /gene="traQ"
                     /locus_tag="ACHKNB_RS10670"
                     /old_locus_tag="pgaptmp_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HGU4195638.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            16541..16939
                     /locus_tag="ACHKNB_RS10675"
                     /old_locus_tag="pgaptmp_002135"
     CDS             16541..16939
                     /locus_tag="ACHKNB_RS10675"
                     /old_locus_tag="pgaptmp_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HGU4195639.1"
                     /translation="MSRFRAMTARLCAAFIYVQLYLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            16996..17247
                     /locus_tag="ACHKNB_RS10680"
                     /old_locus_tag="pgaptmp_002136"
     CDS             16996..17247
                     /locus_tag="ACHKNB_RS10680"
                     /old_locus_tag="pgaptmp_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HGU4195640.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            17162..17980
                     /locus_tag="ACHKNB_RS10685"
                     /old_locus_tag="pgaptmp_002137"
     CDS             17162..17980
                     /locus_tag="ACHKNB_RS10685"
                     /old_locus_tag="pgaptmp_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HGU4195641.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILDDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGMALILLPDSFPDKKYVM"
     gene            18035..18394
                     /locus_tag="ACHKNB_RS10690"
                     /old_locus_tag="pgaptmp_002138"
     CDS             18035..18394
                     /locus_tag="ACHKNB_RS10690"
                     /old_locus_tag="pgaptmp_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195642.1"
                     /translation="MDTLTAGYQFSADAFRHFIIRTLTDDIPPDGVEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            18394..18774
                     /locus_tag="ACHKNB_RS10695"
                     /old_locus_tag="pgaptmp_002139"
     CDS             18394..18774
                     /locus_tag="ACHKNB_RS10695"
                     /old_locus_tag="pgaptmp_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195643.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(18747..19292)
                     /locus_tag="ACHKNB_RS10700"
                     /old_locus_tag="pgaptmp_002140"
     CDS             complement(18747..19292)
                     /locus_tag="ACHKNB_RS10700"
                     /old_locus_tag="pgaptmp_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195644.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTNAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            19517..22561
                     /locus_tag="ACHKNB_RS10705"
                     /old_locus_tag="pgaptmp_002141"
     CDS             19517..22561
                     /locus_tag="ACHKNB_RS10705"
                     /old_locus_tag="pgaptmp_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGU4195645.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            22561..23178
                     /locus_tag="ACHKNB_RS10710"
                     /old_locus_tag="pgaptmp_002142"
     CDS             22561..23178
                     /locus_tag="ACHKNB_RS10710"
                     /old_locus_tag="pgaptmp_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HGU4195646.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            23139..24344
                     /gene="traW"
                     /locus_tag="ACHKNB_RS10715"
                     /old_locus_tag="pgaptmp_002143"
     CDS             23139..24344
                     /gene="traW"
                     /locus_tag="ACHKNB_RS10715"
                     /old_locus_tag="pgaptmp_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HGU4195647.1"
                     /translation="MQRQPATTLLALLIGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            24341..24910
                     /gene="traX"
                     /locus_tag="ACHKNB_RS10720"
                     /old_locus_tag="pgaptmp_002144"
     CDS             24341..24910
                     /gene="traX"
                     /locus_tag="ACHKNB_RS10720"
                     /old_locus_tag="pgaptmp_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HGU4195648.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCHHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            24983..27148
                     /locus_tag="ACHKNB_RS10725"
                     /old_locus_tag="pgaptmp_002145"
     CDS             24983..27148
                     /locus_tag="ACHKNB_RS10725"
                     /old_locus_tag="pgaptmp_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HGU4195649.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAGVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREDKLKNINRDDKDGIKQ"
     gene            27219..27869
                     /gene="excA"
                     /locus_tag="ACHKNB_RS10730"
                     /old_locus_tag="pgaptmp_002146"
     CDS             27219..27869
                     /gene="excA"
                     /locus_tag="ACHKNB_RS10730"
                     /old_locus_tag="pgaptmp_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HGU4195650.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSTSLFILAYGCYFLLFIPPLWLYLKTRAKKKKVLQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTIHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(27934..28143)
                     /locus_tag="ACHKNB_RS10735"
                     /old_locus_tag="pgaptmp_002147"
     CDS             complement(27934..28143)
                     /locus_tag="ACHKNB_RS10735"
                     /old_locus_tag="pgaptmp_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4195651.1"
                     /translation="MSMSPAIANTFLLEMMKDKSKDVTLAVIYALGEGRCQADNIKRE
                     LRRLSESDDMEIKVAAIKALGRLYR"
     gene            28238..28369
                     /locus_tag="ACHKNB_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /pseudo
     CDS             28238..28369
                     /locus_tag="ACHKNB_RS10740"
                     /old_locus_tag="pgaptmp_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
BASE COUNT         7171 a         8171 c         7176 g         6120 t
ORIGIN      
        1 tttctgtatc cagcctccta ccttccccgt ttttatcgtg gaaggcagat atgtttcaaa
       61 aaaaaactcg caaaatgtcc ctcagccggg cactggacaa atacctgaag actgtttccg
      121 tccataaaaa aggtcatctg caggagtttt atcgcgtcaa tgtgattaaa cgtcacccta
      181 tggccgatcg ctatatggat gaaatcacta ccattgatat cgccggttac cgggatcagc
      241 gtttagctca aattaaccca cgaacagggc gtcaaatcac cggaaatacc gtccgtcttg
      301 aacttgccct tttatcatca ttattcaata tcgcccgtgt ggaatgggga acatgccgta
      361 tgaatcctgt tgaactggtc cgaaaaccga aaatcagtaa tggccgcgac cgccgactga
      421 cttctggtga agaacgccgc ctttcccgct atttcaggga caaaaatccg cagctctacg
      481 ttatcttcca tctggctctg gaaacagcaa tgcgccaggg agaaattctc tcattacgct
      541 gggaacacct cgatttgcag cacggcgtgg cgcatcttcc tgaaacgaaa aatggctcac
      601 cacgcgacgt accactctcc cggaaagcac gtaactacct gcaaattctg ccacaacaga
      661 taaacggcaa tgtgttcagt tacacctcgt ccggtttcaa aagcgcctgg cgtaccgctc
      721 ttctggatct gaaaatagaa aatctgcatt ttcacgatct ccgccatgag gccatcagcc
      781 gtctgttcga gctcggcaca ctgaacgtta tggagattgc agccatttcc ggtcaccgct
      841 ccctgaacat gttgaaacgg tacacacatc tccgggcata tcacctggtc agtaaactcg
      901 atgcaaggcg taaacagacc agcaaaattt ccccttattt tgttccctac cctgcaactg
      961 tcgggtgcag aaacgggctg tttgtcgtga cactgcacga tttcgacctt gaaacccgtg
     1021 cagaaacccg ggaactggca atatctcacg ccagcgtatt gttgctgcgc gcactggccc
     1081 aggctgctca gcggggtgaa cgcgtcccca caccaggcga gctacccgca aacattgatg
     1141 aacgggtcat gatttgtccg ttaacaaaca gccagttcat gacaaaagcg aacagtattt
     1201 tcatatcctc ctcctgaata aataattttt gttcaaaatt aacgctatga gatcctttat
     1261 gaaacactta tcctcccgtg cagccactgc ggataataat cgtatcgtta caattgaaga
     1321 cgtttacaca atcgcattct cgcaaccaat tcacaccgaa tcagcgttgg ggcaaaggtg
     1381 gatgttgatc catactggtg aatatgaaag tgccagcgat gcaaaacgct tccttgatac
     1441 gctcgtcacc gggcactgtg cttgcatggt aacaccatca cctgttctgg atcatgacac
     1501 ctggctgcgt atccgcctgc tgatgggcga gataagggat gccaaataaa cattcatggc
     1561 cccggaacat aaaaatagag ccgggaacgc cgggcactgc gtgctgctca gcggagtaaa
     1621 cacgttccca catcaggcga gctgcccgca aacattgatg aacgggtcat gatttgtccg
     1681 ttaacaaact gaaggcatat cagaatgaaa ataaactatt gcttccgggt taaattaatc
     1741 acggtcatcc tgtctcattt cctgatacag atatatatgt ggccagattt tctgacagcg
     1801 aaaaatattc cccgtctcat tgccgctatg gtagctctgt acctgtctgc agtaactgtc
     1861 attccggcat ttttactgtc agatgacagt gagagattta aaaacctctc acctttaaag
     1921 caaacaatat tattagttct gtgtacagtt ggagtaacat ttttactctt acatggattt
     1981 aatacataaa caccttatgg aaaaccccta ttatgttata aataataaaa gactcagagg
     2041 ataacaccca tattgcgaca agcactcgca ttatatttac actttctgta agttcactta
     2101 tataaataaa aacaataaaa aaccaccaca gagaaacaat cattaaagag atacagtaaa
     2161 caaatataca caacacactc atcaccgacc agggtttctc cagcatgaca aaaatcattg
     2221 ataccagcac cacaatagct gcattaccta acagagtttc acgaataata ttccttataa
     2281 tttccagttt ttccttatgc tgttttattt tcatactaac ctcattgatt ttatcccgaa
     2341 aaggaaacga tgatacacca gaatactttc acggcgactc tgcagcataa tgccagacca
     2401 gcggcatgct cagtcacttc ctgtccgaat ccttttcatt cgcccttaaa acagagttgc
     2461 aaacactctt gaaacacact cgatcataac gatcgaaata actcgtttcc gtttaaaaaa
     2521 caaactctct agactcaatt tatgaacaaa acgtgctgcc ccgcatggag gtgaaatgaa
     2581 acctgacagt acaggtattg ccacccgcat gatgctctcc ctggacagag agcgaatttg
     2641 cgaatgcctg ctcagtaatc gccagttgca gtccactccc cttcaggtgc gttatccgca
     2701 gggagtcagg gatgccctgg gtatcatgag cgaacagctt tctctttccg tctctgacct
     2761 gacgcgtatc ctggtcgaag aggcgttaag tgaaatgttt ctccctgcag ataacatcgt
     2821 ccgccgtctt ctgtgcagaa tggaacacat catgcaggca catgatatca gcgcaacaac
     2881 gatggctgca ttgctggccc cctggaatat ccgccctgcc gtcttccggg agccggacag
     2941 actcactgac tatctgaccg gggatattct tgccgcactg gctgactggt tttatctcag
     3001 cccggaatgg ctgaacggca gggtgcatta tccgctgtat cgtccggggg actggcctgt
     3061 cacacaggag gcattctgca ggattatttc ttccggtgag aatgtggaca ttattctctg
     3121 gcatggtttt ccgtttgccg gaacgcattc cggggaatat tgcggtgttc tgctcaggca
     3181 gaaaaaagag attaataatg ccattattta tccggtactt tcgttatatc cggtcagaat
     3241 ggatatggaa aaagaaggct ggtttcagat ggtacgaaaa atatctccgg atatccctgt
     3301 acgcgccgta acgctcactc cggcacaggc agaatatctg ataacaggga aaatattacc
     3361 cactgtgtta ttcagggtct cattatcacc ctggtaataa aactgagtaa taacccgaaa
     3421 taaataacac accaactttt taatggcaca accgtgccct ctgttgttat acccaaaata
     3481 actcaataca ggaaactgaa atgatgatga aaaagcatat tacccgtact ctcatcgcgt
     3541 ctgccgtttt attttccttt aattcgactg cagcaacgtc atattttgaa gcccgcaatg
     3601 atgcgatggg aggaacgggg gttgcctcct ctcactatgg tgtggcacct ctggcaaacc
     3661 cggccctgct gacaaaagcc ggtccggaag atgacttcag tcttctgatt ccgtccgttg
     3721 gtgggcaatt gtccgatccg ggtaatatca gtgacaacgc agacaaaatc agtgatgact
     3781 ggaaagcctt tgatcgtgcc atcgacagtc agagcaacgt gtcgcagact gctgcacgtc
     3841 tgaagacgcg acttcaggac ttccgtcaca cgcatgctga cgcacaggtg ggaacatctg
     3901 ctgtggcgac tcttcccggt gacaccctcg ctgccgctct gatgctgaaa agctacggta
     3961 cggtcagtgt cgacggtaaa gtcagtgatg ccgacctgaa ctatctggaa agcattgcaa
     4021 acagtaacgg tcaggatgtg gataaagaca gactgacctc tcaggctttt gcccgtgccg
     4081 cactggtcac cgatgtgggt attgctctgg ctactgaact ggaaacggcc ggacaaaagt
     4141 ggtctctggg cttcacacca aaattccagc gtgttgacct gtttaactac aacgtgctgg
     4201 ttaaaaatta cgacagcagc gctttcaaag gcgaccgtta ccacaacacc agaaacggca
     4261 ttaacgctga tatcggggcc agcatggacc tggatgacaa ctggacactg ggtctggtcg
     4321 cacagaacct gctcccccgc agtattgaga caaaggaagt gaacggtgtc actgaaactt
     4381 tcaggatccg cccgcaggtg actgccggtg tctcctggca caacgacatg ttcactacag
     4441 cactggatgt tgacctgacc ccagccagtg gcttcacatc agacagtaaa cgtcagtttg
     4501 ctgccattgg tgctgaattt aatgcctgga aatgggcaca gttacgtgca ggctaccgcc
     4561 agaatctggc cagcaatgat ggcagtgcgt tcaccgccgg gctgggtatc tcaccatttg
     4621 atgtcgtcca tattgatgtg gcgggtatag caggcacaga caacaactac ggtgccgttg
     4681 cacaactgca gttcacattc tgatcatctc tgccccggta ctgaccgggg tatccagacc
     4741 tgcatcctcg cgttttccga ccatcaaacg acacagccag acctgtagtg ctgcctctga
     4801 tctggctgtt atctgacata tcattttcgc atgaggaaat gcatgtttaa caaaaataaa
     4861 acgcatatcg aaaacaaagc ctttatggtg gaaccgttcc gggatccgac agctaacgaa
     4921 tgtaatgtcg tgatagcaaa cgatatttgt atccgggtag cactgtcacc ctctgaggtg
     4981 ctccagccat tcagcaaaaa cacgaaccag taaatcctcc ccacaacgct ttcttctttc
     5041 actgctcagg aaaaatacac catgaaccgt atgatacctg cgggatgtct cgccgtactc
     5101 tgcctgaacg gctgccatct ccccccggat aatacagctc cgaccagaaa gtatggtgct
     5161 gcctctgatg tcgccgtcgc ccggcatgcg cagtatcagc tatgggggca gggaatttac
     5221 tctgcagccc ttccggagcg tcagtccggc ggcagtctga aggctcacag cgaccgtgtt
     5281 tcctttgact gggaaggcga tgccctggaa ttgctcaacg aactggcccg tctgcgcggc
     5341 cagcagttca gctacagcgg cgtccgtctc cccttacccg tctcactgca cgtccgcgac
     5401 atgacctttg ctgatgtcct tcgtctcact gaagcccaga ctgcctggcg ggcaacactc
     5461 tcgcagtcag ccgggcgttt acatctgtct tttatgacgc cggagaaaaa atgaaaacac
     5521 acctgtgtct cctgctgtct gctctcctgc ttacaggctg tgccggacag catcaccgtt
     5581 cttctgccgt catgacagaa aacgcaccgc ccccggatat ccgtgcatgg ctgacgccct
     5641 ccggcaacca ggatgatggt atttcagata cccgtcgtca gatgctcacg gatgccggca
     5701 gaacgctggg attccgtggt ggaaaagcac agcgctcatg ggaactgatt caggcactga
     5761 atgcccggga aagtaccctg aatgccctgt atgatttccg gcctctcatc agcaaggaag
     5821 gctggctgcc tcctgttatc gatgaagctc aggatgttgc acacatcaga ccggaccaga
     5881 taagaacagc ctcccgtgtc tggactatcc tccgcccgga acgctttgtc agtaatccac
     5941 ctggatggcg tgactggctg ctgaaaggac tctcaaccac cgccacacca ggcactgaag
     6001 gccgggttgt gccggaagac cgggcacaac gcagactatg ggaaaacgcc ttacgtcagg
     6061 gatggcagga agggcgcctc aacgcagatc tcacactgga agctaaccag aagcgcctga
     6121 cccgtgatta ccggggcatg atgctttacg cccttctgtg gcgtcagggc atgatcacac
     6181 gtccagatgt cacagagcag atacagacgg ttaccggcaa cgggcaaaaa ctcatcactg
     6241 gcgatcgcgt ccgccgcctg aaaacacatg ctgaatttac gctgcagaaa tcgcgctggc
     6301 gtcctcttgt cagcaccgaa ggagagaaaa atgatatcac cagatgactt cggtaccttc
     6361 cccttctccc gttttacggc agaagagttt cgccattttt ttgcctggtg tgcccgccac
     6421 cgcgtcagtg atattgatct gaccggcggt tcaccggtat ccgtcagtcg ctttggacgg
     6481 cgggttcgct catccgtaaa tccccttccg tcctccatca tgatggcact gagtgatgag
     6541 ctgtttgggc gggaggttct tccccgggtt ctgtcaggtc atcccgtgga tcgtaccgtt
     6601 caggtcagcg gagatgcctc cggtcgctat ggcctgcaac gaggtcagcg tgttcgcctg
     6661 cgctgccacc tgatacaggg aacatcggca acagaagaaa aagccatttc cgtcaccatg
     6721 cgggtgatcc cgtcagatat tccggacatc ctcactatga atattgaacc ggatctgctg
     6781 gatgccatgc tcagaaaaac cggcctgggc ttcatctgcg gagaaaccgg ctccgggaaa
     6841 tccacactgg cggcggcact gtatcgccac attctgaaaa atttccccga tcgcaaaata
     6901 gtgacctatg aagatccggt ggaatacatt ctgggcggag agaatgacct tctgcctccc
     6961 catcaggccg aaatcgggcg cgatgtggaa agttttgctg caggacttcg ctctgccgtt
     7021 cgccgtaatc cggacatcat tggtatcggt gaaatcaggg attatgaaac agcagatgcc
     7081 gcagtccagg cgggtaatac cggacacttt tgtatcggca ccatgcacac aaaatcaccc
     7141 ggagaaacct ttgcccgcct gctgggattg tttcctcctg aaatcaggga ttcgatggct
     7201 gcagccacgc tttccctcgt acagttcatt ctggtgcagg tactggtcag aacaaatgac
     7261 ggcggcaggc aggctgtccg ggaatacatc gtcatgactg acgaactgcg caatacgctc
     7321 tccggacaat cccatgcaac atggggacat tacattgacg aaatcattcg tcaggaaaaa
     7381 cggcggataa gggatcaggt tctgaccatg tatcagaacg accgcattga gaaatcagaa
     7441 gcagcgctct tcatcccggc cggagagttc ccccgatgaa acgcgaaggc ttctggcggg
     7501 atgtaacacc accgctcaac atttatggtg tccccctgcc gtttaccatg atttacctgc
     7561 tgttgtttcc gtatccgtca aaaacgacat tctgggtgtg cagcggcatt ctgctgtttt
     7621 ttatgctact gggatattcc ggctggacag tccgcaccct ctgtatacgt ctgtacagcc
     7681 tgcttcaggg taagcaggca tcgggacgtc cctggtggta ccgacatcgc acagaattac
     7741 cccgtgaatg gcacggacca gaatagccct cttttgtcca tcatcccggc gtatcacaac
     7801 gccctcatat ttaatccccg gagaacgaca tgaagtccag atatcgtacc tggcttgctg
     7861 tcccgcctga ggagacagaa gccgtaaaaa atgcagtccc cccgttaaat ggccgcaaag
     7921 cggtagcatg ggatccagag aaaaaactct ggtatgccga ggcgggtacc gacctttcac
     7981 tgctgaagcg ctggttaccc cgtccacagg aaatgtcgat ggatgccggc gatccggtca
     8041 cagaatttcg tcaggttctg gaaaacgccg ggctggttct tgagaaagat ccagaaatgg
     8101 acggagaaat acacagagtg cccacacggg atgacagaag aggctcccag agtggcgcat
     8161 acaaggccta tcttgatggc cgcccctccg gctggtaccg cgattaccgg agcgctgata
     8221 acagtcccac aacctggaca ttctcccgcg cagaaaatat cgatcctctc gccagactgc
     8281 atctgaaggc atccgctcag caaaatcgcg atgagaaaga acgtaaacta cagcaacagt
     8341 acaacaaaca ggccggctat gcccgctcct atcttaacgg gttgccccag gcaacagcac
     8401 atgaatacct gacacgcaag ggcatccggg ccgcgcctgg cgtgcggctg aacaataaaa
     8461 atgagctggt tattcccttc agcaacggcc ggggggaaat acgttcgtac cagcgtattc
     8521 cggtcaccgg cgggaaggat gcccgcattc tgaaaggctc tgagaaaagc ggcaactggt
     8581 tcgccttcgg aaccccggaa aatggccgtc ccctgttatt tgcggaaggt tacgctactg
     8641 ccgcctccct gcacgaagcc accggccatc cggttctgat gacaattgat gccgacaata
     8701 tgattaatgt ggcaaaaaat gcccgcaaaa tctggactga cagccccttt gtcttctgtg
     8761 ccgacaacga ccatcaacgg aaaattaata aaggtgtttc aagtgcaaaa aaagctgccg
     8821 caagcacgca tggcgaagtc attattccgg cctttacgga ggcagaaaaa gcacagggac
     8881 tgaccgattt caatgacctg gacgccagcc ggggacgaga cgcctttcag catgtcatga
     8941 acgcacagtt aaaacatatc ggtatcccca cacccaacag tgacattgcc gaccaccgtg
     9001 aagctgtcgt gatcggcaac ctgatattca caccggtaaa agatgagaag ccacaaatgt
     9061 cccctgaaaa caaacagagc acagccccgg aaacggtgct ggcgacaccg gatacgcctt
     9121 atgacatcca cactgaagag acagaaaacc aggcatatga agatgactat gccgcctatg
     9181 catccctgca gtcagtaact acggagacgt tatccccgga taaaccactg gagaatattg
     9241 cgtctgccgc tgataccggc acagagaata cctctgcggc taccgctggc gaccaggaag
     9301 acctcaatac agacgccaca ggtacagaca ccgctcacaa caccaacaca gcagaaacag
     9361 gaacgcttcc tgaacctgca gagattcagc ctgttcagaa gtcctcccct gaaccaacag
     9421 cagaagacaa acctcaggag agcgttatac ctgccgctga taccggtgaa aaaaatgcct
     9481 ctgccaccac cgctgacaac cagagcgtac aggcagaaga gctctcccca ggtgacgaca
     9541 caccggaaac ctccgtctcc gacgcgaccg ggacacagca caccaccaca acgcagactg
     9601 aagaacaact gcaggtctcc atggccagcg ctgacgactg gagcgcattt gcggaagacc
     9661 tcaacgctga tgccactgtg atgaccacgg ctcaccacac cgatacaaca gaaacagcct
     9721 caccttctga acctgccggg actcagcctg ttcagcaggc aacccctgaa gcaacggcag
     9781 aaaataaacc gcaggaagag gatgttctgt ctgcaacgga taccagtgaa aagagtgcct
     9841 ctgccgtccc cgataacaac cgtgacgcac cgcctgagga gctcaatgcc gatgccacgg
     9901 ttgctgccac tgttcacaac accggcacag cagaaacagc ctcaccttct gaaccagcag
     9961 aaactcagcc agtccggcag gccaccgctg aaccgacggc tgaagataaa ccgcaggaag
    10021 agcgtcttgt gtccgccact gagaccagca cagcgaatga agcagctcgc cctgaaacgc
    10081 catcgctttt cagccgtctg cgccgttttc tgaccggtgg gaaatcacgt acctcagaac
    10141 cttccgttat tcaggagaaa aagcccacgg aggccgaaac tgcgacagag actgcagcac
    10201 caccgtcacc tccggaaacc agcccggaag acacatcgtc agcggaaccg gacacgatta
    10261 tctttgcgcc ccgccgtccc gacagtccgc agcggcacaa cctggacgag atccttaaaa
    10321 atcttgaaca caaagagttt cccgacagga cagcgctcta cagcgttgac ggtgaaccgg
    10381 cattcctgga cagactctac tgcctcgaaa tggtggatgg cgccagcgcg gatgacaaaa
    10441 aggttctggc tgcccttgct gtggccacca acttctatgg cggcgttatc gagctgaccg
    10501 gcagtgatgc ctttaaacaa aaagccatgc agctgattgt cgaatacgac atcaaggttc
    10561 gcatgaaact cccggcccag cgggcagcac tggaaaaact ccgcaaggaa aaagagatgg
    10621 tgcatgatgc cattgtcacc cacagaccca cgccggaact caaccgccag tcaccggaag
    10681 aaacaacaat ggcacaaccg gaacccccta tgtcaccgaa ggcagaggct gaacaaacag
    10741 caggctgtac ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa gctgaagccc
    10801 cccgggccga cagaccggaa caaccagccg ccccgtcgtc agcgccagaa aaagcccctg
    10861 tcagtgcaca gccgcagcaa cccgacacac cggaggaaga gccgcccggc aagctcagcc
    10921 ccggtgaaag tgtgactgcc gtcctgacaa actacggaga aaaagagtat gcgcccggaa
    10981 aagggcgctg cttttttgtg gagctcacca accgcagtgg caaacgggaa tactggggaa
    11041 aaggcctgga agaactggtc aaaaaccacc agaaaggcga ccccgtcacg ctgacattgc
    11101 aggcccggga gagatcacct tcatcaccgg gcaaaccgga atgggtgcga aatatctgga
    11161 ccatgaaacc ggtctgtaac gggatcagcg tatcgcatga taacccgcat gaaggccagt
    11221 acatccggga atatccggcg gccacattcc gccagatgat gaccctgtta cagcagggat
    11281 ggccacagct catgcaggat gtacgtctcc ccgacaaagt acccgacacc ctgtatctgg
    11341 gggaggaccg ccagcccgcc gctgcaccgc gagacccggc actgcgggtt ccacttaacg
    11401 gaacaccgcc cgccacactg acgccgctca tcttctccgt cgacaaacaa agccagcgtc
    11461 tggatctgct gctgttacaa agcgccgacg aatacatgca gggcgtggtc cgcctgaacg
    11521 gcacccttta cccggcactg gccaccccga cagcggacag ccgtcagctg gtcatcaacg
    11581 ccatgaccga caggggcctt cagtttgccg gttacggtga ggccatcaac cgggatgctg
    11641 acggccctaa gcgtcctgcc ccacagttga tgcagtttca cctgaagcaa caggactccc
    11701 cgttgtttgc cgccatccac aaaccggaag agcaaccgga caagcttttc cgctctctgg
    11761 gttttgagca gacctggaaa gagtggtctg acagccaaaa ggcggaggac aggcaggaaa
    11821 aaactctgca acaggcacaa tcccacagcc ccggcctgtg agccggtaag gacgaccatg
    11881 aacatgaaaa aaacgctgct ggtcatactg accagcatct cgctggcttc tccggctgcc
    11941 gtagcagcaa cctgtcgggc aggtgctgcc gcagaagccg gcagcaaagc cgcctgggag
    12001 cgggcgcgca cagccagcca ggcatggtca cagcgagaaa acgacgtcgc ctcctccctg
    12061 cagtcctgcc tgtcccgcat taaggacgtc agcatcactc ttccgcaatt ccccagcctg
    12121 gacaaaatcc ttcgcgatct ggaaaaccag gtctgtgatg ccgtcgtgga tcgggtgaat
    12181 gaacacctgc cggggaatat cgacccctgg aaagactata acctctgaac aggagcccca
    12241 tgagcgaagc acaccccaca tcccctgtct ctccggaaac agataacagc ggagatattt
    12301 ataaacaaac gctcagtacc attgcacagt cagaacaacg ggccgcactg gttcccggac
    12361 tggtcagggc cttgctgtgt agcagcgcag ctctcgtcat cagtctgacc ggcaatatca
    12421 tacagtactg gcataacagc aacgtaacgc gtgaatactt tgccaccgac aatggtcggc
    12481 tggtccgcct ggcccccacc agtcagccag cctggagtca gagcgacgtc atgacgtttg
    12541 gcagcatgac gctgagcgaa gcttttaatc tggattttgt ccactacagg aaacaaatca
    12601 cgacactggc cccccgcttt tccgaagccg gttttgccgg ctatgtgaat gctcttcagg
    12661 cctccaacat tctggacacc attaaaaagg agaaatttaa cctcacagcc accatcgggg
    12721 ccggcacgct ggtgaatcag ggccagatgg aaaacggcat ctggttctgg aaattccagt
    12781 atccggtcag gatgcgtctg gtcggtcaga ccagcacccg ccccgagcag gccttcacct
    12841 ttgaaatcac tgtccggcgc gttgacccac gaatcaaacc cggcggcatg gaaatcagcc
    12901 agatggtttc ccgtaacgcc gcatcaggaa cctgagactc atatcatgca aatacatcat
    12961 cttcttggcg cactgcttat tgtgtgcggc actgtcacgg ctgcagataa cccgggctgg
    13021 cagaacgcac gaaccccaca ggctggcgga aagacaggac aggctcccgg gaacgccccc
    13081 gcaggcggcc ccccgcctgc cacacaactc acaggccccc tgccgccacc tgcctctccc
    13141 cttgttcagg aagccgcatc actggattcc cccctgaatg caggtgaaat tcgggaactg
    13201 cggggacgaa tggccgataa cgaacgggcc atcagcgcac ctgtcaccag tgtggtgccc
    13261 cgtatcagct ccctcacggt aaatctgtca cccggggcca gtctgccgct tgtccgaacc
    13321 gcactgcata acgtcagcgt ggtaacgttc acggacatca acggctcccc gtggcctcag
    13381 tcggatccgc cttataacgc ggcacccggt cactttgatg tgcagttcaa cggcaatatg
    13441 gtctccgtca ctcccctgcg cgcctgggct gcagggaatg taacggtcta cctcaaagga
    13501 ctgtctgtac cggtcattct gaatatcacc agtggtgaaa ccgacaccaa taccctgagt
    13561 caggagatcg acagccgtct ggatctgcgc attccccgtc agggacctgc cagtcctgtg
    13621 gtagccgtac catcagagaa gattgccctg catgatgcca ctctgcaggc ctttctggac
    13681 ggtgttcctc ccgatgatcc caccgtgcgt cggctgaaat tctccggaaa tgttccggac
    13741 accaccatct ggcagcacgg cgatgatctg ctcgtgcgat cgcgggccat gctgcgggat
    13801 gaatttgaac agacgctggc atctgctgac ggtacgcgtc tgtggaaact gccggtgacc
    13861 cccttactca ccttctccgt gaatggtcag tccattcacg tcacccctga actggagtaa
    13921 atcatgagcg cagaacagga cacagccgca aagtccggca aaaaactgtc agccctgctg
    13981 gttctcgggg gcgtgattat catcgggggc ggatacctgc tgctcacctg gctttccccc
    14041 gaaaacagcg catcgctctc gtcagtcaat atcaacagca cgacaacagg cagcgggcgg
    14101 accgtggtgg agtcacagca ctaccgggat ttactgcgtg ctgacaatga gcgcggggcc
    14161 gcagaagcat tacgtaataa ccagacgttc atcgccagtc tgcccagggg actggatact
    14221 gatcacggaa agaatcaaag aagggatact cagcctgctg accaggccga ggggtctggt
    14281 caaccatcaa cgccccctca ggcagatcaa acagcccggg aaaaacagcg ggaagcgtta
    14341 caaaaactgc ttgcccgcat caacacacag catcctgacg cccgcccccc ggtagtggcg
    14401 gctgcaatgt ggggcaccac tggtgctccc gggataagct cacatgcctc accacccggt
    14461 accggcacat caccacaacc acgtctgcag gaggccagtc tggcaacccc ggcagctcgt
    14521 acagggatac agctgatacc ggcactgacc cgcgtacctg ccttcattga tacggaagtg
    14581 gactctgaca atgctgtctc ccgcgtggtg gcctccgtac ctgccggccc atgggccggg
    14641 gccgtcctct acagccagga cgccagactc gccggcaagg gaatggatat ccactttgac
    14701 cggatgatgt ggcggggcat gtttctgaaa gtcaatgcat atgccctgaa tgaacatacc
    14761 ggcatgtcat ccgtggccag cgacgtgaac caccgctggt ttaagcatat cgttctgccg
    14821 tctgtgctcg gtggggtggg cagtgtcggg agtctttaca aagacgccaa cacacaggtc
    14881 attcagggaa actacggcag cattaccggc agggtgggaa tgcctgatgg cgaggcgctg
    14941 gctggcgtca ttgcaggcgg catggccgaa cggggcagtc agttgctgac ccgccaggcg
    15001 gagagcgaac cttaccggca ggtgacagtg tcacgcggag aggttgtctc cattctgttt
    15061 gttgagccgg tcatgagcaa cgatatcagt cccccctcct ccggcactgc cccttctgtg
    15121 ccaccgccct ccggacgccc gctcacccag gaagaagcag agcgtcaggc gtctgacagg
    15181 atccaggcag ctgccatacg taaacagaac gaaatgcggc aacgttacgg aatacaacag
    15241 gaaagtcatc catgaaaaac gaacatgaag atcccggcag ttttggcgaa acaacgacac
    15301 cggcatccgg caaatccttc ctgaaacgcc agtatatggg atacagcctg ccagtctggg
    15361 ccggctgtgt cctcatcatc ctggtcgcag tctggtacaa gtttctgcgg acaccctcag
    15421 agccccctgc agatgcaggc ttcgctgtca caccgcccgc agccgaaagc tgggcagagc
    15481 ccatgcccat gacggagacc gggagcggaa acaccatgtc cgcgcctcct gtctccccac
    15541 aggcagacat gcagcagctt gcccgtgacc tgaaagcaga gctggacagg cgggatgagg
    15601 aaatccgacg taccctgacc atgctgaaag agagcatcac aacccttggc gacaccatac
    15661 ggaaagatga ggcctgggca caggaaaccc gtcagttgct tgatgaaacc cgtaagcagg
    15721 tgcatgccct gcagatgcag ggaccactgc aaatcccgac cgggaataac ggccagtcaa
    15781 cgaaaacccc gccttcccgc ctgcagggca tgcaaatcat gtcggtggaa aaaggaatgg
    15841 cctggatccg ctggcagggt agcgcctggg cagtacggga aggtgacagg ctggggaata
    15901 tcaccatcac ccggattgaa ccgtccagcc gctcagtcat cacctctgcc ggtacattac
    15961 gatgatgagc attgatgccc tgacagccat agagaacttt gccagcggta tattcagtgc
    16021 aggcatggag tttctcttca cctggggaga gctgctgggg attatcggcc tgattggtca
    16081 tctgatgcgt gcccgggctg aagggcgtca cagtatggga ccgggaaaat ttatcgccgg
    16141 tatcgtcatc tgcggcatgc tggtcgccct gccatccttc atcaatgccg ggggtaccca
    16201 gatgggattc agggctgatt ccttcggccc tatcgcttat gtacaaccaa ccacctttgg
    16261 tgccgctgcc ggtgccgcca atgccatgct ctcactggta aaactggccg gagtggggtt
    16321 tgccatgaac ggcatttcca tctggcgtaa atccctgctg gacgggcata ccgccctgag
    16381 cagcagtgag tccatcagca aaggaaacgt caagtttgtt gccggcgttc tgctggtttt
    16441 tatcgatcgc gtcatcgatg cctctctggc gagtcttggt ctcgccttct gatccacatt
    16501 cattttcagt aaatcagtct gttagttaag gagacactat atgtcacgat tccgtgccat
    16561 gacagcacgt ctgtgcgctg catttattta tgttcagtta tacctggtgg cctgccgtga
    16621 ccggctgata cgcctgttac ttctgcttcc cgtgatactg ctgccccaca cagcgatggc
    16681 aaacaccctc ttcgatatga tcaaaaccgt tggtgatggt gcaaaaggct ccagcagttc
    16741 atttgtggct atcggtcagg ttattggcct ggtgctcttt atcggcggcc tgcttggcct
    16801 caaacaaatc ggtaaaaaac ccggagcagg gctggcctgc ctgctgtcaa ttctggtcgg
    16861 cctctgcctt gccgtcgtac cggaactgat gggtagcgga cagaaaacgc tcggtctttc
    16921 tccggtttca atcagttaat ccgcaaaggc accgggaaac cggtgccgtc ttcacactat
    16981 gaggaggacc agaccatgtc ctgtgagctg ccccctttca ccctgctcag aacagagcac
    17041 ggcaacgtga ttgcccgcga caaccgggat aatcaggagt ttgtgtttgc agagtgttat
    17101 tccaccgcca tcgcagcact gattctggct gccgtcaccc tgtatacaga gagaaaaacg
    17161 tatgaacaaa acaccgccac tcatcgcccc ggcccgggaa cgctggcttt tcccgctccc
    17221 gcaaccggac acggtattca gcggtgaatc cctgctgcca ccacccgtgc tctcaacacc
    17281 cggacgctgt ccctgctgtc gcaggaccgt cactcaccgc ttcattctgg atgattcatg
    17341 gccattacag cagatggctg acacctgccg ggatacggtc gtcctgctgg aaaaaaacct
    17401 cacccgggtg atgcgcctga aaaaacatcc ggtaccggaa aacgctgacg aaaaaaagaa
    17461 acacacccga accctgcagg atgcggagcg gtccctggca caggcccgcc tgtcggccag
    17521 gcgactggcc ctgcgtcatg tggaaaagtc acaaatcgtc acaacagatg cactcagcga
    17581 aaatgaaagt gaactgctgc agcctgaagg cccgcccttt catctctgtg ccttctgcca
    17641 cgcctggcac tgcctgaacg gctacgctgc cgcacagggg gtgatggtct ggctccctga
    17701 tttgcatccg gcctcggtag ttgctctcaa tgcacgggca ctgaaggaaa ttttcagcga
    17761 tgagcgtaag cgaatcagac agggacgggc cgtactgaat gccctggtac agaaccgcct
    17821 ggccgttgag gagaaatttc gcacctggcg tccggcggat tttgctgatg cactgcgccg
    17881 ctggccgccg gcacagcgtc aaatgttgcg ggagaaaatg gacggaatgg cgcttatcct
    17941 gctgccggat tcgtttcctg acaaaaagta cgtcatgtga cacacaccgc tgccggtgac
    18001 gttctgccgg ccccaactga cagaggaaca aaaaatggat accctcactg ccggatatca
    18061 gttttccgca gatgcattcc ggcactttat tatccggacg ctgacggacg atattccgcc
    18121 agatggagtt gaaaaagaga ttgcccggtt cattctccgc cacccggcat ttaacggcgg
    18181 ctggctgctc tttctggctg cagaaattca tgaaagatcc cgtacccata acagcatcac
    18241 cgtcacagtg agcgtcgata actatctctc atggatggat attgccgtgt ccggtctccc
    18301 ggaattaccg gatatgccct tctgtgattt tttcattcgc cgccagcagt ttatttatcc
    18361 gggtgtaccc ccgacaaaat caggagaaga atgatgaaaa ttcaccgcat atccccggag
    18421 acgcttataa cactcatcct cgcacacctg gccggaaaag ctgacagtac cgccaaagaa
    18481 gagcaccggc tactccgtcg gttccttcgg gatgatgacg gcaggctggc cggcatactg
    18541 ctgaatattg ccggaatact tcagttcaac agggaattaa gcgcccggca taattatccg
    18601 gccacacccc tgactgaatt ttcattaagg aaacggggaa aacaacttca tctctgtctc
    18661 tgcagtctcc gtttctttta tattcctccg gtatttatac aaaacaaaag aagaaaatct
    18721 attgttgtac atcttaataa gatcacttat aaacaaaccc attcaatacg ctgacgaagt
    18781 gattcatact gattatactc atctttgcct atatgttttt taagcgtatt aacaatatca
    18841 tattgaggat gtcctgaatt gtcagtatac agtgttgttg caggaaggac tgcataatct
    18901 gatatttcac aacctgtata cagttctttg tcagttcggc aggaatggca aagagaaaga
    18961 catgtataca ggtctgtgtt ttctgcctcc tcaggagata accacgacac atattgcaaa
    19021 tcatctgtat caggataaca attttcttcc tgaacctgaa ctctgaaaat atagaaaaat
    19081 aattcatctt ttgaaaggca tgctatttta ttcccccaat gcaccgcatt atcaagttga
    19141 tccgataaat aaatccatcc tggtgtcgtc tctaacgacc agcatataac agaaggagca
    19201 ttagttctta ttttcccatc ttttattatt tgcaggcctc tgctcattgt tgtcgcatga
    19261 aaaagattta atattttatt ttcagccttc actctatttc ctcatttccc aagaaacata
    19321 ttaaaaacgg aaaacattac acataacctg gaggacaaat catgacttta aatttccctg
    19381 ccggggaaac tacactattc tcagcaaaaa acatcctgac agcaatactc actctgcaca
    19441 acttacacgc atagcgttcc catttgaata cagttgcagc ccgcgattta ttttgtcttc
    19501 cggagccacc tctgccatga atatcaaccg cattattttt gccattgaag actgtatcag
    19561 taccctgtca cggtattccg tttccgcatt cttccccgat tactgtgatc tgcacaccgt
    19621 catcggtctg gaccagcagg accgcgaacg ccgcccctgg ctgaaagccc cctacattgc
    19681 caccacaaaa caggggtatt acctttccgt ctttgaaatc agcggtgccc tgaaagaaat
    19741 ggatgaaaac ccggacctga cagcccccgg cacactggaa tcactgatta cagcgctggc
    19801 agagcgaatg aacactgcct ataaaaacat cggtcacaaa atcagttttg ttttcgaacg
    19861 cgatccggag cgtggaagga atgaaatcga agccatgctc ttaccccagc gaaaatccat
    19921 cgcacgcacg gggatcctgc ttcaggacgt tctggatgaa aaagtcacca cgctcacccc
    19981 ctggcttgtc cgggaacgct gctggctgac cgtctggagc tctgaagaac tgctgagccg
    20041 gaccgatcgc aaggaccacc agacccgtat caggaaactc gctgaacacg ctccgccagc
    20101 ccgatttgcg caggatccat ggcgctggac actttccgca ctcaaaatac ggcatgacgc
    20161 ctttctggac acgctggagc aggccctgac acatgattct gatggtctgc tggttcgcct
    20221 gatggatatt catgaagtgg gacgggaaat ccgccgtcaa ctggagcgca acagcacacc
    20281 tgcagtatgg cagcctcatc tgcctgaaga tgcccgtcct gccggctggc gacagggcga
    20341 ggatacctct gtattccacg caccgtcgct gaatctgcag ttattcacca cacagcctga
    20401 aacacacggc agcctgataa aggccggtga cctctggcac ggtatggtcg ccattaccct
    20461 gcccccccag aatctgcgga cattcaacca gctggtcagg gatgtgcccc gcgcaatccc
    20521 ctggcgtatc cggatggatc tgatgcccgg cggcatgaag gcgcttgggg tcaagaaaac
    20581 cctgctgacc tacagcagct ttatttcccc tctccggccc atgtatgact cagtgatgat
    20641 gctgaatgac atcaacaaac atgatccggt ctgtatcatg actattgttg ccaccacatg
    20701 gggaaacagc cgggaagtct gtacccgcaa ccaggcgctc ctgaaatctg cgctggaagg
    20761 ctggggagta tgcggcacca ccaccacctt cggtgatccg agacgtgcct gggtgaacac
    20821 ggttctggcg gcctcccacg gttccggtcc agtccccctt tacccgcccc tttcacacgc
    20881 cctttccctg tttcccctga acagggccgg ctctgtctgg cggggacagg gaaatctgat
    20941 gatgcacacc gaagacggct ctgcatggga ggtcgcgctg gcatcgtcac aacagaacaa
    21001 gcacacggaa ctgacgcccg gcgcacccgg tctgggcaaa tcggtactca tcaacgcact
    21061 gtcggagatc cagatagcct cagcccagaa gaacctgcct ttcattgcct atatcgacaa
    21121 gggattcagt gcacagggac tggtacagct tatccgtgac agcctgccgg agcagagaaa
    21181 ggacgaagcc gtggggatca ttctgagcaa tgatccggaa cacacccgta acctgtttga
    21241 tgtgatgtac ggtgctaaaa aacccgccac cccggaaaag aacttcatgg tatctgtact
    21301 ctgcgcactg tgcgtggata ccggcaccgg tcagccctgt aatcccggtg acacccggca
    21361 aatcataagc aacctggtgg atctggcctt cagggagtat ggcgaaaaca accccagact
    21421 gtaccgtgca ggaacagagc cccttgtgga tctcgccctg gaagagagtg gcatcgcaga
    21481 acaacatgac gctggatggt ggaatgctgc cacctggttt gaaatcaggg acatgctgca
    21541 tatggctggc aatatccccg cagcccagcg ggcacactac caggccatgc ccctgctggc
    21601 tgaaatgtcc gccctgctgg gacagccatc catccgggat gtgttcggca cagttcagcg
    21661 cgataactca gcagaattgt tactggacta catccgccgt gccctcgatc agggacacag
    21721 tgactacccg atgatgtccg gctgtacccg gtttatgctc agccccgaca cccgcgttgt
    21781 ggccgttgat ctgaacaatg tggccggtga caagacacct gccgggcgtc tgcgcactgg
    21841 catcatgtac ctgctggcag gacaaatcgc cggcggggat tttgtcctgc cgcagtatca
    21901 ggaggaaata cggaaaaatc tcgacccacg ctaccatgag gtgattttcc gccgcattga
    21961 acagcttgat caggaggtca agaccaaagt ctatgacgaa ctgcacaatg caaaagggat
    22021 caatttcatc tgggaagccc tggacaccca ggagctggag cagcgtaagt tcgggatacg
    22081 cacagtgctg agcacccagc tactgcagga ctatccaccg gcagttctca aatccgccaa
    22141 caccctctgg ttgctgcgtt accgccctga cgaaattccg tttctgcggg ataattttgg
    22201 tgtccctgaa gtcaccctgc ggcgcttcct gaaaatgccg gaaggggctg caccggatgg
    22261 cagcggtgtc ccggtactgg ctgtgttccg tgtaaaaaac ggtacgctgg cccggatcct
    22321 gaaattcact ctcggtccac tggagctgtg ggcactgaat tcctcaccca aagacagcgc
    22381 cctgagacgt gcgctgacgc aggagctcgg gtcactgcgg gcacgccaga tcctggcaga
    22441 aaactttccc cgtggttcag ccacctcact gatagaacac cgcgcccgta cacatgacag
    22501 tgaaaacgtc atccatgatc tggctgcaga actcatccgt aagcagggat atcacctctg
    22561 atgtacagca tacacatcac tccccgctgc cggcgtcctg cacggggaca ggtcttcctg
    22621 aaaacgctgc gcgtggacgg tgacagtttc agcttcaccc tgccgtccgc ctgcttcacg
    22681 ctgcgtccct cccccaatcc ccgggtcatt ttcgccagtg acagacgggc tgacggggaa
    22741 atgagtggcg ttcgcctggc tctgcccgga gagacaggca cgctgattac aacggccacc
    22801 tggcaggtca acgtgccgga gcggcctcct tttgatatac agcatgtcat tcactggctc
    22861 ctgccctcgc ctctcacggc gctgatgtcc gacaacacct ggctgtggcc ggatagcccc
    22921 ggcacaccgg cactgacggc gggagactgg ccggtaatgg aaaccgatac cccgacgctg
    22981 aaacgcgtga agcgtcgtac cttcacgctc atcgataacc gcaaccgtca gggatggatc
    23041 acccgtcgct tccagtgctt cccgctggct ccgctgccgg aatcacacag tcaccgcgta
    23101 ccagacatcc tgtatcccct gaaaacggag aaaaaagaat gcaaagacaa ccagcaacca
    23161 cactgcttgc actcctgata ggctgtgcgg ttatcccgtc cacccacgcc tattctgtga
    23221 ccgtagtggc cagccagcct gtcaccacac aaatcatgcc ggagctggcc gccattcagg
    23281 gcgtgatggc agagatggtc cgtatgcaga cagccaccgg cacagccatc aaccagagtt
    23341 cagagaaact ggcctctgtc attgcgcagg atgggcaggc cacccgtcag cagatgatat
    23401 tcagtaatga aacccgccgg cttgaggaag cccggaagag cttcagcgtg ccggactcca
    23461 tctgcagtga atccgcctcc ggagttgcag ctgaaagtcg ccgtgcagca gcgtctgcgg
    23521 ctgcacgtct gagtcaggga ggaggcgtca gcagtaaacc aatccgtgaa cgtctctccc
    23581 gggcagcaga ctcaccaacc cgcgaggcat atgacagtgc aggcattcat gccggttact
    23641 gcacggaagc tgaatatgcc cgctttggcg gaaccgatgt ctgccctgcc gtgggcgagc
    23701 tgccgggcgg agacagccag gtgcgatcgc tctatcaggg tgcaggcaca gcagataccc
    23761 ctgccgccct cacctgggat cagaaacaga ttgatgctgc cacggcttac atgaaaaaca
    23821 ccgccagacc gtcagcgggt cgtgccccgg ggaaaggtga agtgggaaca cagaccgggc
    23881 gcacgtatgt gggcctgcag aatgaataca acggcatcat cgatgccgct tctcatccgc
    23941 aactgtcact gatagctgac agtaccccta atgaggccac ccgtggagcg ctgactgaag
    24001 ccctgcagtc accatccgct gcggcatatt ttgaccggac cgcatcatca gaggcccgca
    24061 cccgtggtca catgagccag cgggagtttg aggcctttga agctggcaga cgctatgcaa
    24121 acaccgactg gcagcaggat ctgcagggca tggaaggcga taaccttctc cgtgaactgc
    24181 tgcgcactac cgccctgctg aactggcaga tgaacgacct gaaagagcag atacgccagg
    24241 gtaatgttat cgccgggcag cagctggcac tggcggcacg acagtattac ggacagcgtc
    24301 tcggtgaact gtcacaggcc atgagccagg ggagcgtaag atgaaagacg acaacagcac
    24361 cgacaaaaaa aaaccggccc cccgtctccg ccgcatcggt gacatcggga taaacctgtt
    24421 cgcaccactg gcagaaaccc ggtggatgct gcgcagctgc caccatatgc tgggccagca
    24481 taaaacgcgc ctgaagcgcc tcatgcccga ccggcaaccg gagagtccgg tctgtatgac
    24541 ctgggaagaa gccgtcaggg ccagtggcct tacggtcaac gaactggatc ggcgtctgcg
    24601 acgccgcagg atgttctggc ggacaggatt ctggctgatg atgataccgg tacttctgct
    24661 gtgcggcatg gcccttgctg ccacagcgct tccggccatc accctgatac gtatactggc
    24721 ctgcctgtta ctgatgagtg ccattgcagc actgtgtgcc tcaaaaaccc tgattgtgtc
    24781 ataccgcctc tggcaactga atgaacggcg ggtcagcgag gacgaacagg gcaccttccc
    24841 cgattacctg caggcccggc acggctggaa acaggccaca tggctggcca ttacccaccg
    24901 gcagtactga ccgtccggtc tgcaattcct tttcctcatt ccggaggctg atgccctgca
    24961 tcccttcggt tccggagtta tcgtgaaaat catacccaga gcactgagtg cgggtctggc
    25021 gctggctgcc gtacctgcac aggcattcgt cacctatcag gacattgtga gcgccgccac
    25081 caatccggat gacctgtcgc gccaggcact ggtcaccatt tttggcgacg tggtcacaaa
    25141 tcccctgaca gccagcagca acaccattat cggtaacctg ttcatggtgt ttaacgcaat
    25201 tatcgccatt atggggatag tctggttcct gtttattgga atccgccatg ttgtccgcac
    25261 cggccaccag ggcaatgtct ttaacaacgg acgggacgtc gtcggaatgc tcagtattgt
    25321 gagcggtttt atgatgattg tgcccaccgc ttccggctgg agcctggcac aactggtcat
    25381 gctctggggc gcatccatca tgggcattgg ttccgccaat atcatggttc agacggctgc
    25441 agaaaacatc gctgaaggct attcaatgac cgtacagcca gttcatgtct ccacccgcac
    25501 ggcagcacgg ggaatactgg agatggcgct gtgcaaacac gccatgaatg caggcctcga
    25561 tgactttaac cgtactgccc gttcatcaac accccccatg agcgaatcct cacggacaga
    25621 gaacggcagg tataccgtca ccgtttcaaa tggcagtgcc acctgcggga gtgtgagtct
    25681 caccgtgaaa gggaatggca gcaacaacca gtccgcgctg gactattttt tcaatccctt
    25741 cattgaaacg gagtatcagg gagtcctgac ggcacaacgg tctgccatgg ataccatgct
    25801 ccgtgatctg gacagcgcag cacaggaatt tgtgtcagcg taccggaccc gacgcgacac
    25861 gggtaacggc aacctgccgg atattgaaag ccgtattcag aaggctgcca accggtatga
    25921 agaaacggtg cagaaggcgt tgccaacaga taacgcagaa actgagcgca gggagcagct
    25981 gaaatcctac ctgaccacct acggctgggt tgccctgggg gcctggtacc agacctttgc
    26041 caccgccaac cagcgactgg cagaactggc cgaccgtgcc cccactgtga gtggagtttc
    26101 atcgcagggc gaagccggag atactgaatt cctgaaggaa gtaaaaggag cttaccaggc
    26161 acagttgcaa aactcaccat atacacctgc actgggaaca gccacctcag ccagtgaata
    26221 ccaggcatct aatacacaga atcctcaatc agccctgtta gagttcatag ggccagggtt
    26281 aactcgattt tcaaataaag tagcaaccat acattcagga acaggaacaa cgagtcagca
    26341 ggcaaatcct ctgctgaaaa tgaaggacat tggtgattac accatggtca caaccgaaac
    26401 catctgggcg ggttatacca ctgtcagggt gctttcaact gccggcagca actccgtttt
    26461 tggccgactg gtcaatgccg tcagcggctt atctgatgca gcaaaggctt ttctggaagc
    26521 ctccgcacca ccactctact tcctgctgtt tttgctgttc tgtgccggtt tttcgttgtc
    26581 catttacctg ccgtttatcc cgtttatctt ctggatgacc ggcatcggca actggatagt
    26641 cagcgtactg attggatgcg ccgccggccc cctgtgggcc gcaacccacc tgggaacaag
    26701 tcaggacaga ggaagtcggg ccgcttacgg ttacatctac ctgatagaca gcatgatcag
    26761 gcctctgttg atggtgttcg ggttcttttt tgcatcggtc gccatcgtgg ctggcggcac
    26821 catactgaat gctctgtacg ctgcagcact aatgaatgtc cagataaatt ctgtgacggg
    26881 ggtgttcagc atggcgggat ttttgatgat ttatgcccgc gcctgtacca cactggttgc
    26941 gggcgttttt gcacttcagg cttatctgcc agaccatgtg attaattttc tgggtggacg
    27001 tgacggcgtt aatatgctgg ggaatatggt ttcttccgtc aaagatatta ttgccggcgg
    27061 aaacaccaat atacgcagaa cacctggcgt tcgtgaagat aaactgaaaa atattaatag
    27121 agatgataag gacggaataa aacaatagag tacaaaacag tattgccata ttcacctgaa
    27181 gagtttaaaa tatattcagg tgaatatgga ggtttattat gaacaaaacg aaaagcagat
    27241 atccttcctg gtgggcatat taccacttgg cccgcaacgc ttatttctta ttaggaatac
    27301 cgtgtttact tttatactca gccgcttata catcagattt attagatgat aatctaagac
    27361 attcaacaag cctttttatt ttggcatatg gatgttattt tcttcttttt attcccccac
    27421 tctggctata tctgaaaacc agagcgaaga aaaagaaagt cctgcaggtt gttcagcaga
    27481 taaaagaaag cagtcccttt gcgccgacca gcgattacga gcaactgtca ttctcgaaga
    27541 gttgttattt cgggatcgac attaaaaacg gcaccatgct gtatgtgcgc atttacccaa
    27601 acaatgtcat ggatgtgatt ggtctggata ttcacaactt cacccgaaca gttgctgaag
    27661 acggcaagct ggaaatccac accacctatg tgagtctgcc gatgatccct cttgaaatct
    27721 ccggcatctc agcaaggacg ctggccaaca ccatacacac catggctgca cgtggatatg
    27781 agtacaaaga gcgtttcccg cagatgattc ggtatcgggt taaagaatgg gaaaaagttg
    27841 ccggtgtgcc tgttgcagaa gtgttttaat acggctcata catccggttc atctgaaccg
    27901 gattttcttt actacaacag ataacttata tcgttatcga taaagtcgcc caagagcctt
    27961 gatagctgca actttaatct ccatatcatc actttcactt aatctacgta attccctctt
    28021 tatgttatct gcctggcatc gaccttcacc taatgcatag atcacagcaa gtgtaacgtc
    28081 tttcgattta tccttcatca tttcaagcaa gaaagtatta gctatcgcag gagacatact
    28141 cataataatt tcctctttat aaaattaaca aagatactca tttagatgta taaacaccgt
    28201 ataataaaat aattcttgct aaacaagaaa ttaaaatctt gcatccacag tctcgcacac
    28261 catgaagaca ctcggatatg agtacaagga acgtttcccg cagatgatcc ggtatcgggt
    28321 taaagagtgg gaaaaagttg ccggtgtgcc tgttgcagaa gtattttaag gtagcgacca
    28381 actgttagac ggtaatccgt cagccggttt tctctgaacc ggctttttct tttcaggcaa
    28441 aatgcctgtc tgccatccct gtatcagact gtaattagca caatacggca atttgccgtt
    28501 cagtatattc caggaggtac aaatcatgcc atctgcaaca caaactgccc gactggaagc
    28561 aaggatcagc acagaactgc atgctatgct gaaacgcgct gcagaaatcc aggtaaaaaa
    28621 gggcgcattg aggtttgc
//
LOCUS       DAZFVO020000054        28419 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFVO020000054 DAZFVO020000000
VERSION     DAZFVO020000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.054"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1209
                     /locus_tag="ACHKNB_RS10745"
                     /old_locus_tag="pgaptmp_002149"
     CDS             <1..1209
                     /locus_tag="ACHKNB_RS10745"
                     /old_locus_tag="pgaptmp_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4195652.1"
                     /translation="TVNDDANDVAVTESYKPVWDWKISQFNVSDIKFETMINPQFADR
                     TYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLRIMAEGENFLSQLLNIEDE
                     VLNILLRENFNEQLDTNVNTIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGD
                     RFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGI
                     KDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPITVSIDEMTNALDPVELAAIINKK
                     HHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGG
                     RERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMP
                     LIRRRTMLQL"
     gene            1399..1512
                     /gene="yhiY"
                     /locus_tag="ACHKNB_RS10750"
                     /old_locus_tag="pgaptmp_002150"
     CDS             1399..1512
                     /gene="yhiY"
                     /locus_tag="ACHKNB_RS10750"
                     /old_locus_tag="pgaptmp_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HGU4195653.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1575..2642
                     /gene="yhiI"
                     /locus_tag="ACHKNB_RS10755"
                     /old_locus_tag="pgaptmp_002151"
     CDS             1575..2642
                     /gene="yhiI"
                     /locus_tag="ACHKNB_RS10755"
                     /old_locus_tag="pgaptmp_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4195654.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2639..5374
                     /gene="rbbA"
                     /locus_tag="ACHKNB_RS10760"
                     /old_locus_tag="pgaptmp_002152"
     CDS             2639..5374
                     /gene="rbbA"
                     /locus_tag="ACHKNB_RS10760"
                     /old_locus_tag="pgaptmp_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HGU4195655.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5374..6498
                     /gene="yhhJ"
                     /locus_tag="ACHKNB_RS10765"
                     /old_locus_tag="pgaptmp_002153"
     CDS             5374..6498
                     /gene="yhhJ"
                     /locus_tag="ACHKNB_RS10765"
                     /old_locus_tag="pgaptmp_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4195656.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6602..6832
                     /locus_tag="ACHKNB_RS10770"
                     /old_locus_tag="pgaptmp_002154"
     CDS             6602..6832
                     /locus_tag="ACHKNB_RS10770"
                     /old_locus_tag="pgaptmp_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4195657.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6829..7188
                     /locus_tag="ACHKNB_RS10775"
                     /old_locus_tag="pgaptmp_002155"
     CDS             6829..7188
                     /locus_tag="ACHKNB_RS10775"
                     /old_locus_tag="pgaptmp_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4195658.1"
                     /translation="MIKLKTPNFMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7308..7709)
                     /gene="nikR"
                     /locus_tag="ACHKNB_RS10780"
                     /old_locus_tag="pgaptmp_002156"
     CDS             complement(7308..7709)
                     /gene="nikR"
                     /locus_tag="ACHKNB_RS10780"
                     /old_locus_tag="pgaptmp_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HGU4195659.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7715..8521)
                     /gene="nikE"
                     /locus_tag="ACHKNB_RS10785"
                     /old_locus_tag="pgaptmp_002157"
     CDS             complement(7715..8521)
                     /gene="nikE"
                     /locus_tag="ACHKNB_RS10785"
                     /old_locus_tag="pgaptmp_002157"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HGU4195660.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8518..9282)
                     /gene="nikD"
                     /locus_tag="ACHKNB_RS10790"
                     /old_locus_tag="pgaptmp_002158"
     CDS             complement(8518..9282)
                     /gene="nikD"
                     /locus_tag="ACHKNB_RS10790"
                     /old_locus_tag="pgaptmp_002158"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HGU4195661.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9282..10115)
                     /gene="nikC"
                     /locus_tag="ACHKNB_RS10795"
                     /old_locus_tag="pgaptmp_002159"
     CDS             complement(9282..10115)
                     /gene="nikC"
                     /locus_tag="ACHKNB_RS10795"
                     /old_locus_tag="pgaptmp_002159"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HGU4195662.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10112..11056)
                     /gene="nikB"
                     /locus_tag="ACHKNB_RS10800"
                     /old_locus_tag="pgaptmp_002160"
     CDS             complement(10112..11056)
                     /gene="nikB"
                     /locus_tag="ACHKNB_RS10800"
                     /old_locus_tag="pgaptmp_002160"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HGU4195663.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11056..12630)
                     /gene="nikA"
                     /locus_tag="ACHKNB_RS10805"
                     /old_locus_tag="pgaptmp_002161"
     CDS             complement(11056..12630)
                     /gene="nikA"
                     /locus_tag="ACHKNB_RS10805"
                     /old_locus_tag="pgaptmp_002161"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4195664.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12741..13328)
                     /gene="acpT"
                     /locus_tag="ACHKNB_RS10810"
                     /old_locus_tag="pgaptmp_002162"
     CDS             complement(12741..13328)
                     /gene="acpT"
                     /locus_tag="ACHKNB_RS10810"
                     /old_locus_tag="pgaptmp_002162"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HGU4195665.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13383..14432)
                     /gene="yhhT"
                     /locus_tag="ACHKNB_RS10815"
                     /old_locus_tag="pgaptmp_002163"
     CDS             complement(13383..14432)
                     /gene="yhhT"
                     /locus_tag="ACHKNB_RS10815"
                     /old_locus_tag="pgaptmp_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGU4195666.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14564..15781
                     /gene="yhhS"
                     /locus_tag="ACHKNB_RS10820"
                     /old_locus_tag="pgaptmp_002164"
     CDS             14564..15781
                     /gene="yhhS"
                     /locus_tag="ACHKNB_RS10820"
                     /old_locus_tag="pgaptmp_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195667.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15785..16342)
                     /gene="dcrB"
                     /locus_tag="ACHKNB_RS10825"
                     /old_locus_tag="pgaptmp_002165"
     CDS             complement(15785..16342)
                     /gene="dcrB"
                     /locus_tag="ACHKNB_RS10825"
                     /old_locus_tag="pgaptmp_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HGU4195668.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16415..17080)
                     /gene="yhhQ"
                     /locus_tag="ACHKNB_RS10830"
                     /old_locus_tag="pgaptmp_002166"
     CDS             complement(16415..17080)
                     /gene="yhhQ"
                     /locus_tag="ACHKNB_RS10830"
                     /old_locus_tag="pgaptmp_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HGU4195669.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17301..17546
                     /gene="tusA"
                     /locus_tag="ACHKNB_RS10835"
                     /old_locus_tag="pgaptmp_002167"
     CDS             17301..17546
                     /gene="tusA"
                     /locus_tag="ACHKNB_RS10835"
                     /old_locus_tag="pgaptmp_002167"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HGU4195670.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATPRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17648..19846)
                     /gene="zntA"
                     /locus_tag="ACHKNB_RS10840"
                     /old_locus_tag="pgaptmp_002168"
     CDS             complement(17648..19846)
                     /gene="zntA"
                     /locus_tag="ACHKNB_RS10840"
                     /old_locus_tag="pgaptmp_002168"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HGU4195671.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLQLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19920..20546)
                     /gene="yhhN"
                     /locus_tag="ACHKNB_RS10845"
                     /old_locus_tag="pgaptmp_002169"
     CDS             complement(19920..20546)
                     /gene="yhhN"
                     /locus_tag="ACHKNB_RS10845"
                     /old_locus_tag="pgaptmp_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HGU4195672.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20687..21046
                     /gene="yhhM"
                     /locus_tag="ACHKNB_RS10850"
                     /old_locus_tag="pgaptmp_002170"
     CDS             20687..21046
                     /gene="yhhM"
                     /locus_tag="ACHKNB_RS10850"
                     /old_locus_tag="pgaptmp_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HGU4195673.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFSLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21049..21318)
                     /gene="yhhL"
                     /locus_tag="ACHKNB_RS10855"
                     /old_locus_tag="pgaptmp_002171"
     CDS             complement(21049..21318)
                     /gene="yhhL"
                     /locus_tag="ACHKNB_RS10855"
                     /old_locus_tag="pgaptmp_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HGU4195674.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21308..21904)
                     /gene="rsmD"
                     /locus_tag="ACHKNB_RS10860"
                     /old_locus_tag="pgaptmp_002172"
     CDS             complement(21308..21904)
                     /gene="rsmD"
                     /locus_tag="ACHKNB_RS10860"
                     /old_locus_tag="pgaptmp_002172"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HGU4195675.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22054..23550
                     /gene="ftsY"
                     /locus_tag="ACHKNB_RS10865"
                     /old_locus_tag="pgaptmp_002173"
     CDS             22054..23550
                     /gene="ftsY"
                     /locus_tag="ACHKNB_RS10865"
                     /old_locus_tag="pgaptmp_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGU4195676.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23553..24221
                     /gene="ftsE"
                     /locus_tag="ACHKNB_RS10870"
                     /old_locus_tag="pgaptmp_002174"
     CDS             23553..24221
                     /gene="ftsE"
                     /locus_tag="ACHKNB_RS10870"
                     /old_locus_tag="pgaptmp_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGU4195677.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24214..25272
                     /gene="ftsX"
                     /locus_tag="ACHKNB_RS10875"
                     /old_locus_tag="pgaptmp_002175"
     CDS             24214..25272
                     /gene="ftsX"
                     /locus_tag="ACHKNB_RS10875"
                     /old_locus_tag="pgaptmp_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGU4195678.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25517..26371
                     /gene="rpoH"
                     /locus_tag="ACHKNB_RS10880"
                     /old_locus_tag="pgaptmp_002176"
     CDS             25517..26371
                     /gene="rpoH"
                     /locus_tag="ACHKNB_RS10880"
                     /old_locus_tag="pgaptmp_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HGU4195679.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26642..27745
                     /gene="livJ"
                     /locus_tag="ACHKNB_RS10885"
                     /old_locus_tag="pgaptmp_002177"
     CDS             26642..27745
                     /gene="livJ"
                     /locus_tag="ACHKNB_RS10885"
                     /old_locus_tag="pgaptmp_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HGU4195680.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27944..28327)
                     /gene="panM"
                     /locus_tag="ACHKNB_RS10890"
                     /old_locus_tag="pgaptmp_002178"
     CDS             complement(27944..28327)
                     /gene="panM"
                     /locus_tag="ACHKNB_RS10890"
                     /old_locus_tag="pgaptmp_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HGU4195681.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6767 a         7363 c         7845 g         6444 t
ORIGIN      
        1 acagtaaatg atgatgctaa tgatgttgca gttactgagt cgtacaaacc cgtctgggac
       61 tggaaaatat cgcaattcaa tgtatctgat attaaattcg aaacaatgat aaacccccag
      121 ttcgctgaca gaacctattt ctccaattgt tcacctgttg atcccacaag taccagacca
      181 acctattttg gcgataccga tggctcagtc ggtgcagttt tgtacgcgct ttttgcaacg
      241 ggacatttga gaatcatggc tgaaggagaa aatttcctta gccagttgct taatatagaa
      301 gatgaagttc tcaacatatt gctaagggag aactttaatg agcaacttga cactaatgtt
      361 aatacaataa tcagtattct gaacagaaga gatatagttt tagaaagtct tcagccttat
      421 ttagttatta ataaagacgc tgtgacgcca tgtacgtttc ttggcgacca aaccggcgat
      481 cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg cataatgagt
      541 atcaatgaaa atgttcatgt attagctgga aatcatgaaa cgaattgcaa tggtaactat
      601 atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgctgg gataaaagat
      661 tatcctgtat gtttctatga ccccaaatac aaaataatgg caaatcatca tgggataact
      721 tttgatgagc agcgaaaacg ttacattata ggaccgataa cagtctctat tgatgaaatg
      781 accaatgctc tcgatccggt cgaattagcg gcaattatca ataaaaagca tcatgctata
      841 attaatggca aaaagtttaa aactagccgg gctatctctt gtcgatcctt taatcgttat
      901 ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg ttcacaaatg
      961 ttaggtataa atcaagttgt tgcacataac ggaaatggtg gaagagaacg cataggcgaa
     1021 acggggacag ttttaggtct taacgcccgt gacagtaaac atgctggaag aatgttcagc
     1081 atgcataatt gccaaattaa ccctggtgca gggcctgaga taaccactcc ctggaagtct
     1141 taccagcatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg cacaatgtta
     1201 cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag actgttgata
     1261 aaacgtaaaa aggaatgctc tgttcctgca actgaacagg cgtgaaacgc tatccaacag
     1321 gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg cgtgtttcat
     1381 ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa gccttccccg
     1441 ttggcgctca atgctctgcg cgctggtcgt atttgccgtt tccttcttat cccggatgga
     1501 agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct tacagggatt
     1561 atggcgggtc gattatggat aagagtaagc gccatctggc gtggtgggtt gtcgggttac
     1621 tggcggtggc ggctatcgtg gcgtggtggc tgttgcgccc ggcaggtgtg ccggaaggct
     1681 ttgctgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc aaaattgccg
     1741 ggcgtattga caccattctg gtgaaagaag gccagtttgt tcgagaaggt gaagtgctgg
     1801 cgaagatgga tactcgcgtg ttgcaggaac agcgactgga agccatcgcg caaatcaaag
     1861 aagcacaaag cgccgttgct gccgcgcagg ctttgctgga gcaacgccaa agtgaaactc
     1921 gcgccgctca gtcgctggtt aatcaacggc aagctgaact ggactccgta gccaaacgtc
     1981 atacccgttc ccgctcgctc gcccatcgtg gggctatttc tgcgcaacag ttggatgacg
     2041 accgcgccgc cgctgagagc gcccgagctg cgctggaatc ggcaaaagct caggtttcgg
     2101 cttctaaagc cgctatagaa gcggcacgca ccaatatcat tcaggcgcaa actcgcgtcg
     2161 aagccgcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc gaactgaaag
     2221 ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggcgaagtg ctggcggcag
     2281 gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgactttc ttcctgccaa
     2341 ccgaacaggc gggcacgctg aaactgggcg gtgaagcccg gctgatcctc gatgccgcgc
     2401 cagatctgcg tattcctgca accatcagtt ttgtcgccag tgtcgcccag ttcacgccaa
     2461 aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa gcgcgtatcc
     2521 caccggaatt actccagcag catctggaat atgtcaaaac cggtttaccg ggcgtagcgt
     2581 gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg ttgccgcaat
     2641 gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg tgagccagca
     2701 ttatggaaaa accgttgcgc tgaacaatat cacactcgat attccggccc gttgcatggt
     2761 cgggctgatt ggcccggacg gcgtcgggaa gtcgagcttg ttgtcgttga tttccggtgc
     2821 ccgcgtcatt gaacagggca atgtgatggt gctgggcggc gatatgcgcg acccgaagca
     2881 tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca aaaatctcta
     2941 ccacacctta tcggtgtatg aaaatgtcga ttttttcgct cgcttgttcg gtcacgataa
     3001 agcggagcgg gaagtccgaa ttaatgagct gctgaccagc accgggttag caccgtttcg
     3061 cgatcgtccg gcaggtaaac tctccggcgg gatgaagcaa aaactggggc tgtgctgcgc
     3121 gttaatccac gacccggaac tgttgatcct tgatgagcca acaacggggg ttgacccgct
     3181 ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga gcaatatgag
     3241 cgtgctagtc gccaccgcct atatggaaga ggccgaacgc ttcgactggc tggtagcgat
     3301 gaatgccgga gaagtgctgg caactggcag cgccgaagag ctgcggcagc aaacgcaaag
     3361 cgccacgctg gaagaggcgt ttataaatct gttaccgcaa gcgcaacgcc aggcgcatca
     3421 ggcggtagtg atcccaccgt atcaacctga aaatgcagag attgccatcg aagcgcgcga
     3481 tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc gcattccacg
     3541 cggggagatt tttggttttc ttggatcgaa cggctgcggt aaatccacca ccatgaaaat
     3601 gctcaccgga ctgctgcccg ccagcgaagg tgaggcgtgg ctgtttgggc aaccggttga
     3661 tccaaaagat atcgataccc gccgtcgggt gggctatatg tcgcaggcgt tttcgctcta
     3721 taacgaactc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc acatcccgga
     3781 agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt tttaagctca acgacgttga
     3841 agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc tggcggtggc
     3901 ggtgattcat cgcccggaga tgttaatcct cgatgaacct acttctggcg tcgatccggt
     3961 ggcgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca aagtaaccat
     4021 ttttatctcc acccacttta tgaacgaagc ggaacgttgc gaccgcatct cactgatgca
     4081 cgccggaaaa gtgctcgcca gcggtacacc gcaggaactg gttgagaaac gcggagccgc
     4141 cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcaggacaga gcaacgaagc
     4201 cgaagcgccg cccgtgatac acgacaccac ccacgcgccg cgtcagggat ttagcctgcg
     4261 ccgtctgttt agctacagcc gccgcgaagc actggaactg cgacgcgatc cggtacgttc
     4321 gacgctggcg ctgatgggaa cagtgatcct gatgctgata atgggttacg gcatcagtat
     4381 ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac cagaccgtca gtagccaggc
     4441 gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgccgc tcaccagtta
     4501 tgacgagctt gatcgtcgga tgcgtgcggg cgatataacg gtggcaattg agatccctcc
     4561 caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggcgtct ggatcgacgg
     4621 agcgatgccg agccgtgccg aaacggtaaa aggttacgtg caggcaatgc accagagctg
     4681 gctacaggat gtggcgagcc gacaatccac acccgccagc caaagcgggc tgatgaatat
     4741 tgagacgcgc tatcgctata acccggacgt aaaaagcctg ccagcgattg ttccggcggt
     4801 gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg tggtgcggga
     4861 aaaagagctt gggtcgatta tcaaccttta cgtgaccccc accacgcgta gtgaattttt
     4921 gcttggtaaa cagttgccat acatcgcgct ggggatgctg aactttttcc tgctctgcgg
     4981 cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc tcaccctggc
     5041 ggcgctgctg tatatcatca ttgccaccgg aatggggctg ctgatctcca cctttatgaa
     5101 aagccagatt gccgccattt tcggaacggc gattatcacg ttgatcccgg cgacacagtt
     5161 ttccgggatg atcgatccgg tagcttcgct ggaagggcct ggacgttgga tcggcgaggt
     5221 ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag cgctggatct
     5281 gactgatttg tggcaacttt ttatcccgtt gctgatagcc atcccgctgg tgatgggctt
     5341 aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa tatttttaat
     5401 ctgggtatca aagagttgcg cagtctgctc ggtgataaag cgatgctgac gctgattgtc
     5461 ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta cgccaggatc gttgaacctc
     5521 gcgccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat cgttaacagc
     5581 ttctatcgtc cgtggttttt gccaccggag atgatcaccg ccgatgagat ggatgccgga
     5641 ctggacgccg gacgctatac cttcgcgata aatattccgc ctaattttca gcgtgatgtc
     5701 ctcgccggac gccagccgga tattcaggtg aacgtcgatg ccacgcgcat gagccaggca
     5761 tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag ctttgtcgcg
     5821 cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg ctttaacccg
     5881 aacctcgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat taccatgctg
     5941 gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac ggtggaacac
     6001 ttactggtga tgccgataac gccgtttgag atcatgatgg cgaagatctg gtcgatgggg
     6061 ctggtggtgc tggtggtatc gggattatcg ctggtgctga tggtgaaagg tgtactgggc
     6121 gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgctcag tctgtttgcc
     6181 accacgtcaa tcggcatttt tatggggacg atagcgcgtt caatgccgca actggggctg
     6241 ctggtgattc tggtgctgct gccgctgcaa atgctttccg gtggatccac gccgcgcgaa
     6301 agtatgccgc agatggtgca ggacattatg ctgaccatgc cgacgacaca ctttgttagc
     6361 ctcgcgcagg ccattctcta ccggggtgcc ggattcgaaa tcgtctggcc gcagttcctg
     6421 acgctgatgg caattggcgg cgcgtttttc accatcgcgc tgctgcgatt caggaagacg
     6481 attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc ataaagtgaa
     6541 gtcataaggg aatgtcatgt aaacacaggt acaggcttta cgaaagaaac aaaaaaacac
     6601 actggatcag ctatttaaat cacctttacc tcaaggaatc aaatggactg atatagaatc
     6661 tctggttaaa gcgttaggcg gagaaattaa ggaaggtagg gggtcgcgct gtaagttcat
     6721 actaaatatg agcgttgcgt gtttccatcg gcctcatccg tcgccagata ccgataaagg
     6781 cgctgtagaa agcgtgcgtg actggttact aagtataggg gtaaaaccat gatcaaactg
     6841 aagacgccaa actttatgga gattgccggg caacctgctg ttatcactta cgtgccagaa
     6901 ctgaatgcat ttcgtggcaa attcctgggc ttgtcaggtt attgtgattt tgtctctgac
     6961 agcattcagg ggctgcaaaa agaaggggaa ctttccctgc gagagtatct tgaggattgt
     7021 aaagctgcgg gtattgagcc gtatgcgcgt acagaaaaga ttaagacctt tacgttacgc
     7081 tatccagaat cgctgagtga acgccttaat aatgcggctg cgcagcaaca agtctctgtt
     7141 aatacctata ttattgaaac actgaatgag cgtttgaatc atttataaat tttagcggcg
     7201 tacattacgc agaatatctg cctttacagg atctatctat gcaactgaac ttaattttat
     7261 aaattaagtt cagttaatat cttttctttc ctcgtttcag aaataactca atcttccttc
     7321 ggcaggcact gcaaatgccc gtgccgcacg ccgcgctggg cgataacgtc atcggcaaaa
     7381 tgctgcacgt cgcccatatc acctttcagc acggcgattt ccagacagtc gtcgtgattg
     7441 atatgcacat gcagcgtggc gacggagaga tcgtggtgat ggtgctgggt ggagacaatg
     7501 cggctggcta agtcgcgttt ttcgtgttca tacacatagg agagcaccgc gaaaccttgc
     7561 gtgccgtgct gctgagtggc ctcctgggcc agggcgctac gcagaatatc gcggatagct
     7621 tcggaacggt tgttataacc acgacgctgg ctcaggctgt ccagcgtctc cagtaaatcg
     7681 tcatcaagcg tgatggtgac tcgttgcatt tgcgttaaac cttttctgtg gtgcgacggc
     7741 gcacggggaa tgcgggtaat accgcgtttt gtagcacacg tccggcgtca gaggaaaagg
     7801 ttaatttatc tcccaccacc tgggtttcga cgatttgtcc gttgtccatc accatcaccc
     7861 gctgacagaa gcgttccacc aggcgtaagt cgtgggtgat gaacaggcag gcggtgccaa
     7921 actgttgttg tagctttttc agcaggcgaa tgacgcccgc ctgtaacacg agatcaaggt
     7981 tagaaacggc ttcatccaga atcagtagtt tcggttcgac cgccagcgcg cgagccaggc
     8041 agacgcgctg gagctggccg ccgcttaact gcggtgggcg tttgtcgaga acgctgtcat
     8101 cgagatcgac cgccttcagc atttcgctgg cgcgcgccag ttgttcggat tttttcagtg
     8161 atagcaggtg gcgcatcggt tcacgcagga tctcgcgcac ggttttgcgc ggattcacgg
     8221 cgctgatgga gtcctgaaat accatctgaa tatcgcggcg gaacgcttta cgctgggcgc
     8281 gattgagttt cgccagcggt tcgccgcgcc agctaatatt cccttgtgag ggagactcta
     8341 agcctaccag cagccgcgcg agggtgcttt ttccgcagcc gctgcgtccc agcagggcga
     8401 cggtttcgcc gcttttcagg gtcagggaaa cgttattcag caccgcctga tgttgatgtt
     8461 ttccgctaaa tccaccgtgg gcatagtgat gggaaaggcc gcagacgtta agtaaggtca
     8521 tgatgccagc tccataccgt agagggcgag atgagcggaa accaggctgc gcgtcaccgt
     8581 atgtttgggg gcgttaaaca gcgtttctac atcgccctgt tcgacaattt taccttgtga
     8641 catcaccgcc acgtcatccg ccagacgcgc cacaacgccc atatcatggg tcaccagcag
     8701 cattcccggc gcttgttttt gcataatgct ttccagcaga tcgaggatgc gcgcctgtgc
     8761 taccacgtcg aggtcggtgg tcggttcatc ggcgatgata aacggtgatt cacacagcac
     8821 cgccatcgca atcatcatgc gctgcaacat gccgccgctc atctcgaacg ggtacagctt
     8881 cagcacgcgc gcggcgtttt ccagccccac cgcttctatg gcagcggtaa gcgtagcgtc
     8941 atcggcgggt ttccctaacg ccaggcaggt ttcccgcgcg tgggtgtgca tggtgtgcag
     9001 tggattaaag gcgctgcgcg ggttctgcat gatggtggca attttgatgc cgcgcagggc
     9061 gcaaggcgaa accggtttgc catcggctaa aatttccccc gccgtctggc gaacgccagc
     9121 gggcaaaatg cccagcgtcg cggcgcaggt taatgatttc ccgctgccgc taccgccgac
     9181 taacgccagc acgcgcccgc gttgcagggt taacgataca ccgtgtacca gcggctgcgc
     9241 ggcctgtagc gcgatattac gtagttcaat ctgttgcggc attagtgtgc gtgctccgtc
     9301 accagatgag gatccagatg atcgcgcagt gcgtcaccca ccaggttaaa ggccatcacg
     9361 ctgataaaca gcgccagccc cggccagaac atttgcagcg gctgggtcca gatatactgg
     9421 cgcgcgtcgt taatcatcac gccccattcg gcggtcggcg cggtcacacc gaggccaagg
     9481 aaagacatcc ccgcgacgtg cagcatcata tggccgatat ccagcgttgc cagcaccagc
     9541 agcgaaggga tcaccgcgcc tgccagatga tcgacaaaca cccgcacatg gcccgcaccg
     9601 gaaagccgtg acgccagcac aaactcgcgt tggcgtagtg aaatcaccag gctgcgcacc
     9661 atgcgtgcat accacgccca gtgcgacagg gcgatggcga taattacgtt ggtcagcccg
     9721 gtgccgagca cgccaaccat aaagaacgac agaatcgagg tcgggaaggt cataaacata
     9781 tcggcgacgc gcatggtggc ctgatcaacg cgtccgccaa tcagtccggc gctgccgcca
     9841 atcaccagcc ctaatgtcag caccagcagc aggcaggcca ttaccgaacc gagcgacacg
     9901 cgggtcgccg ccatcagccg cgagaaaata tcacgcccta agtgatcggt gcccagccag
     9961 tgctgcgcat ccggcgaaag caggcgcgac ggcagatcaa tagcctgagg gtcatacggc
    10021 agccaccact ggctggtaag cgcaatcagc gccagcaggg cgataatgat cagcgccagg
    10081 cgtaccgacc agcgggaaga gaggaaaaag ttcacgcgtg cgctccttca tgacgacgaa
    10141 tgcgcgggtc cagcgcggcg ttgagcaaat cgacaatcaa attacagacc acaaaaacca
    10201 ccaccatcat cagcgtaaag cactggatca ccggatagtc acggttaaaa atcgccgaca
    10261 ccgcatagcg cccgacgccc ggccaggcaa agatgttttc gataatcatc gtcccgccga
    10321 tcagttcgcc gatgtgcatc cccacggcgg tgatcatcgg cagcgaggca ttgcgcagga
    10381 tgtggcgacg ttcggtctgt ttgtcgttca ggccgcgcag acgcgcccag gtgacgtgac
    10441 gctgaccggc gacgtccagc atactggcgc gcagtaaacg cgcgttaatc gccagcgaca
    10501 taaaggcaat ggaaaccgca ggcaaaatga tgtgctgcca gccgccgtaa cccatcgcgg
    10561 gtagccattg cagatacacc gaaaacgcca tcaccagtaa aaacgccagc cagaagttag
    10621 gcatcgacac gccaagaaac gcgatgaaac gtacggcgaa atccggcaga cggtcgcggt
    10681 ggcgcgccgc ccagataccg agcggtacgg aagtgagcag aattaatacc agcgccgcac
    10741 ctgcaagttc cagcgtggcg ggcaggaagt tcagcatatc gtccagtacc gggcgttggc
    10801 tggcgaatga gataccaaag tcaagatgca gcgccttcca caaccaggtg ccgtactgga
    10861 cgtacagcgg ctgatccagt cccagcatgg tgcgggtaga ggccagcatc tccggcgtcg
    10921 gcggcaggtt agacagacgc aaataatcga gcgccgggtc gccggtaccg aggcgcagca
    10981 tcagaaaaat gatcaccgag gcggcgagca ccatcgggat cagcagcaga aagcggcgta
    11041 atacgtaacg caacattaag gtttcaccgg tttaatctgt tcgaacggaa tttcggtggc
    11101 gatcggcgcg taggggatat tacccagctc cggttttgat accaccatca tcgagatgta
    11161 gctgataggc agataaaccg cctcgtcatg cagacgggtc agaatgtcgc gatacagcgc
    11221 ctgacgttgc gtttcgtcat gggtcgccag cacttcgccg atctctttat caatcagcgg
    11281 cttatcggct aatccttgct gtgcctggaa gtcagcgtgc gacggtacgc gcattgaact
    11341 caggaaggcg tgtggatcgt atggcgcgcc ccaggtgcgg tggaaaatca tgccaaaacg
    11401 accgtcgcgc tgacgagcat agatactgct ctcttcttcg ccaatcagcg agacatctgc
    11461 gccaatctgg cgcatatcag cctgaatgat ttccgccatc gatttgctta acgcatcggt
    11521 gccgatgaac gacagttcaa tgcgcagcgg ctgaccattt ttctcgcgga tgtctttgcc
    11581 cgcaggcagc gtccaaccgg ctttttccag caacgctttc gctttctgcg gatcgtactg
    11641 gcgcggtttc aggccgagat tagcgtaagg cacagaaggg gcaaacaggg tgtcggcgac
    11701 ctgctgggtg ccatataacg cgttatcaat cagcgatttt ttgtttaccg cgtaattaag
    11761 agcttcacgt actgccagct cgttggtggg ggctttggcg gtattgagcg ccagcatcac
    11821 ggtttcgatc ggctgtgaca gttgggtgtg gtaagccgga ttctggctaa agcgggcgaa
    11881 ggtatcgagc ggtaataacc cttcgtttcc gtacagcagg tcgatatcgc cagtttcaaa
    11941 cgccaccgcg cgggtagtcg ggtccgggat gacgttaaag gtgatctttt taatcgctgg
    12001 cttttcgccc cagtagtttt cgttacggac gaagacatcg tactgattca gtttcgattc
    12061 ctgcaaaatc catggtccgg tgccaatcgg cgctttaatt ccgttcatgg tttcatggtt
    12121 tttaaactgc gagggagcga taaaacggaa aggacggggc agggccagtt cttgcaggaa
    12181 aggatagtag gcgcttttca gggtaatttg cagctctgtt ttactgagtg ctttaacatc
    12241 aacaatctgg tttgccagct ccagccaggc gtgacgttga cggttatcga gcactgcgcg
    12301 gaagttttct gccgccgcct cggcatcgaa cggttcaccg ttggagaatt tcacgtcatc
    12361 acgcagggtg aaggtccagg ttttaccatc ttctgaatga gtccagcttt ttgccagcca
    12421 cgggatcacc gaaccgtctg cctgatattt caccaatggt tcataaacca tgctctgggc
    12481 gaacatctgg ttaggcgtgt aaaggtgcgg gtttagtggc ccgacattca ccggccaggc
    12541 ggtagtgatt tcatctggtg cagcggcatg gacgataaaa gacgcacaag ccagcagcgc
    12601 aaatagagtg cggcggagtg tggagagcat gataacccca atggattaaa atagatggcg
    12661 gaaataagta tgacgatttt aagtattcgt catactgatt acctgttctg gatcaataga
    12721 tgggcagtcg ggtgggcgga tcagttaact gaatcgatcc attgcacact gtcggcggtg
    12781 agcgtaaagg gggtgggggt gcagatcgcc aggcttaaat tttcaagctg acaatggctg
    12841 actgacagcg aggagtgata ggtgctgtct acgctgacga tttgccaggc gctgccgcca
    12901 cgctgtttaa cgatggcttc tttgcgcgtc cagatgcgcc agaacatttc cagttgctga
    12961 tcaggatgca cggcgtccat ctcagcgtgt tccccgaggc tgaatacggc gttcgccagc
    13021 cagcgccagt tggcgcgcgg gcgaatcact tcgatatcgc agccgacttc gccttcatca
    13081 ctcaacagca gagcgatatc gtcaccgcta tggcttaagt tgaaccatag cggcgtttcc
    13141 ggcgcgaatg caggtttgcc ttgttcgcca tagatgatct ccggtagcgg ggaaagcgtg
    13201 tgcgaaagca atgcacgccc cgccagccag cgttcgcgtc gtggaccttg cggtgcttgc
    13261 tcgcgtaaac ccggtggcag tggagctgcg cttaaggtcg aaactttccc cagaactatc
    13321 cgatacatat cagggccaac gtttaatgga aaatgaaagt gcgtatcgta tcacttgtcg
    13381 cctcatcccg gtaaccgact tttcggtctg cccggcccca gtaaaatcgc cagtttgctg
    13441 ccacctttcg tcgtttccat ccagatttta cacacgctgg ttaatggcac cgaaagtagc
    13501 atccctaccg ggccgagcag ccatccccag attaacaatg aaagaaatac caccatggtg
    13561 gacatcccca ggcgatggcc catcatccgt ggctctaaaa tattgccgat gaccatatgg
    13621 acaactaaaa acaatgcgcc gaccagaata cattcgtaaa taccattaaa cagcagcacc
    13681 tgaatcattg gcggtacggc ggaaattacc gcgccgatat tgggcacgta gttaagcaaa
    13741 aacgccagta ctgcccacat cagcgcaaac tgtaccccca tcagctccag ccccagccag
    13801 acgatgacac ctgtccataa actgagtagc gtcttcaatg caagatagtg cgaaacgcct
    13861 ttaagtgcgc ggtgtaatcc cgcgatgtga atctgtggat tattcagcgc aaaacgcatt
    13921 ttgtaaggga cgtggcgcac ttcaaacagc ataaaaacta cggtcatcac cagcaaaagc
    13981 acgctcgcca ttgccccgga aagcccggtc attagcgctg tggtgaaggt aaccactttt
    14041 tccgagtcca tccgctgcag cattcgctcc ggcgacatat gcaaattaag aaaaggcaac
    14101 atctcctgca atttaaaaag tttgcgcgtc agctccttat taaacttcgg cagcatagag
    14161 ataaattcgt taaacgatgc cgccagtacg ccgaccagcg cggttagtgc gatcagcatc
    14221 accaccacta caatcgtaat ggcaacgggg cgttgtactc cccgacgaat aaaccaggtg
    14281 acgagcgggt tgaggacgat ggcaaaaaac agcgccagta acagctgcac aatgatatct
    14341 gccgctgcgt gaatgcccgc gaggatcact accagcgagg ccagcttgag cagaatgtgc
    14401 atgcccgttt tatcgggttg aggggtttcc attggggctt ccttgtgact ttttgtatta
    14461 agtgtagcgg gagtcacgcc agcaatattc tgattcttac tgctgatttt cacgtcagca
    14521 catggtaaaa atgaaacact gttgtaaaaa tgtggtgatc ctcatgcccg aacccgtagc
    14581 cgaacccgcg ctaaacggat tgcgcctgaa tttgcgcatt gtctccattg tcatgtttaa
    14641 cttcgccagc tacctcacca tcgggttgcc gctcgctgta ttaccgggct atgtccatga
    14701 tgtgatgggc tttagcgctt tctgggcagg attggttatc agcctgcaat atttcgccac
    14761 cttgctgagc cgccctcatg ccggacgtta tgccgatttg ctgggaccca aaaagattgt
    14821 cgtcttcggt ttatgcggct gctttttgag cggtctgggg tatctgacgg caggattaac
    14881 cgccagtctg cctgtcatca gcctgttatt actttgcctg gggcgcgtca tccttgggat
    14941 tgggcaaagt tttgccggaa cgggatcgac cctgtggggc gttggcgtgg ttggctcgct
    15001 gcatatcggg cgggtgattt cgtggaacgg cattgtcact tacggggcga tggcgatggg
    15061 tgcgccgtta ggcgtcgtgt tttatcactg gggcggcttg caggcgttag cgttaatcat
    15121 tatgggcgtg gcgctggtgg ccattttgtt ggcgatcccg cgtccgacgg taaaagccag
    15181 taaaggcaaa ccgctgccgt ttcgcgcggt gcttgggagc gtctggctgt acggtatggc
    15241 gctggcactg gcttccgccg gatttggcgt catcgccacc tttatcacgc tgttttatga
    15301 cgctaaaggt tgggacggtg cggctttcgc gctgacgctg tttagctgtg cgtttgtcgg
    15361 tacgcgtttg ttattcccta acggcattaa ccgtatcggt ggcttaaacg tagcgatgat
    15421 ttgctttagc gttgagataa tcggcctgct actggttggc gtggcgacta tgccgtggat
    15481 ggcgaaaatc ggcgtcttac tggcgggggc cgggttttcg ctggtgttcc cggcattggg
    15541 tgtagtggcg gtaaaagcgg ttccgcagca aaatcagggg gcggcgctgg caacttacac
    15601 cgtatttatg gatttatcgc ttggcgtgac tggaccactg gctgggctgg tgatgagctg
    15661 ggcgggcgta ccggtgattt atctggcggc ggcgggactg gtcgcaatcg cgttattact
    15721 gacgtggcga ttaaaaaaac ggcctccgga acacgtccct gaggccgcct catcatctta
    15781 aaacttactg aataaccagc gtattaatga tgttttctgc ggtggtctgc gctttttgct
    15841 gatcgtcagc gggcagcgta atttgcatgg tcagcagttg attacccacg ttacccagaa
    15901 taacggaaga gtacgccgtc tggcctttcg cggagataat actgtctaac tgctgcattt
    15961 tgtgaccttt cagctcaatg gctttattgg ttaccacttg cagctgcgga tcgcggctac
    16021 gttgctgatc ttccagacgc ttcgccagca ccgccagatc ttctttcgga tcatcgccca
    16081 tgatgacgat gactgctttc tgcccggtgg cgtcggacca gacatgcatg ttattggcct
    16141 gcgttcccag cttaccgctc tggtcggtca tatccgctgg cagcgagaaa cttaacttgc
    16201 catcaagcag gttgacggga ttcccggtag cgttactttc cgcgaccgaa ccctgcgccg
    16261 tagcgttagt gtctttatca tcacaggccg caagccccat aaccagcagg ccaattccga
    16321 catatttaac cagattgcgc attgacttct tcctttcgat aaacggccat aacggctcat
    16381 tcatccatct tatcacaact ctgataacga acctttaact cgcctgcaaa gcgttgattt
    16441 cggatttatc tgccagtctt ttcaacagca tattgagtaa tacgccatac attggcagga
    16501 agaaaacgat actgattaac actttgaaac agtaatcgac cagcgcgatt tccatccagt
    16561 gttcagccat aaaggcatcc gggctacgcc agaaggcaat gaagaaaaag gccagcgtgt
    16621 cactgacgtt accgaacagt gtggacgctg tcggtgccag ccaccagcgg cgactttgac
    16681 gcaggcggtt aaaaacgtgc acgtcgagga tttgccccag cgcgtaggcc atgaaactgg
    16741 cggtggcgat acgggcgaca aacaggttga agtgggcgag tgcgccgaat ccctgccatg
    16801 aacccatata gaacagcgac gagatgacat aggagattaa taacgcaggg atcattaccg
    16861 cgaagataat gcgtcgggcc agcggtgcgc caaaaatacg cacggtcagg tcggtcgcga
    16921 gaaagataaa cggaaaacta aacgcgcccc aggtggtatg aaaaccaaaa atggagaccg
    16981 gaagctggac cagatagtta ctggaggtga tcaccagcag atgaaataac gataaccaga
    17041 acaacgcctt atagcgttga gtttgcgaga aaacgttcat attgtacctt tttgattaac
    17101 cattggggtg agggaaccca atacgtacga cacgtctgtt atcttaacat aataacgtgc
    17161 ttaccctttt ttcgagccgc cgcatgatac tgctttacgt tgacaatgca atggttaatt
    17221 ttcacgcaat cgttaacctg gtttgcttac ggactcacag ggcgtaaact agcgccgttt
    17281 ttttaagtga tgagaagaaa atgaccgatc tcttttccag ccctgaccac acactcgacg
    17341 cgcttggcct gcgctgcccg gaaccggtga tgatggtgcg caaaaccgtg cgcaatatgc
    17401 agcctggcga aactttactg attatcgccg acgatccggc gacgccccgc gatattcctg
    17461 ggttttgtac ctttatggaa cacgaactgg ttgcgaaaga gacggatgga ctgccttatc
    17521 gttatttgat tcgtaaaggc ggttgatggg gaaagattgg cttcgatgcc gccttttccc
    17581 ctcaccctaa ccctctcccc agaggagcga ggggaccgat cgcgctcaat gttgcgatcg
    17641 gtttgcctta tctcctgcgc aacaatctta acgcattcgc tgttaccagc acggtcgcac
    17701 cggtatctgc cagcaccgcc agccatagtc cggtcatccc tagcagcgtg gtgacgagga
    17761 agatcccttt cagccccagc gcaatagtga tgttctggcg gatattggcg tgagtggcgc
    17821 gtgccagttc aatcatttgc accaggccgc gcaggtggtt atgggttaat gctgcgtcgg
    17881 cggtttccag cgccacgtct gtgccactgc ccattgcaat cccaatggcg gcagctttca
    17941 tcgctggcgc gtcgttaata ccatcaccga ccatcgccag gggcgcatgt tgattcagct
    18001 tggtcaccgc tttgacttta tcttccggca ataggcccgc tttaaactcc agccccagct
    18061 ccccggcaat tgccgccgct gcgcgtggat tatcgccggt gaggatcact cctttaaccc
    18121 ccagcgcgtt cagttcactg atggcagttg ctgcgtcggc gcgcagggta tcctgcaatg
    18181 caatgatacc cagcacgtca tcattacgta ctaccagcac aaccgtttgc ccggcgcttt
    18241 ccagttcatt aatcaaacca gcaaatgcat cagcgggatg tttcccggca gcgcaaatca
    18301 atacgcgctc accgttaacc tgcgcttcaa tgccagaccc gaccagcgcc cgctgtgatt
    18361 cggcggtggg aatggcgagt tcagcaacct gtgcttcgcg tacgatggcc tgcgccagtg
    18421 gatgcgtcgc gccttgctcg accgccgccg ccagtgtcag cagttcagat tcactaatac
    18481 ccgttgccgg atgaatcgcg gtaacgcgcg gtttaccgac ggtcagcgta ccggttttat
    18541 caaacgccac ctgagtaaca cgacccagct gttccagcgc cgctccgcct ttaatcaacg
    18601 ccccacgacg cgctgccgcc gccagcccgg aggtaatcgc cgcaggcgtg gagataacta
    18661 acgcacacgg gcagccaatc agcagcagcg tcagcccttt ataaatccac tcctgccagc
    18721 tggcggcaaa cagcagcggt ggcaccagcg tcaccagcag ggcgacggcc ataatcgctg
    18781 gcgtataaat acggctgaaa cggtcgataa accgctcaat gggggcgcgg cgctcttcgg
    18841 cttcttcgat cagtttcaga atccggtcaa tggcgctggc acccggttct gacagcactt
    18901 ccagcgttac cagacggtct acgctggtgg ctcctgcagg aactttatcg cccgtcgcgc
    18961 gctccaccgg aatggattct ccggtcaggg cgctttcatc aaaactggca aacggtgaga
    19021 gcagtttacc gtcggcaggc aaacgcccac ctgcggcgac ttcaatcaca tcgccaggcc
    19081 gcaggctgtt aatcgccacc tcttcccgct caccgttacg caggcgcgtg gcggtttctg
    19141 gtttcagcgc cattaacgcg ctaacgccct gacgcgcgcg gctggcggcc cagccttcca
    19201 gtcgttcacc aatcaaaaac agcagcaaca ccatcgcagc ttcagccgtt gcgccaataa
    19261 acagtgcacc aatagcggct acgctcatta aggtttcaat ggcgaagtag ctgccggatt
    19321 tgatcagccg taatgcctga cgagcaatcg ggtacagccc aaccagcgtg gtcgcgataa
    19381 acgccagttg cccaaacgga tgattaaatt gctccagacc ccagctgatt gccatcatca
    19441 cgattagcgt aatcagctgc agattctctt tcaggcgcga tgcttgtggc tcgtcggcgg
    19501 cctgctcatc gcgcagggaa tagcccgctt tttgcaccgc agattcaacc tgggcacgga
    19561 tgtcattgtc ggcatcgacc accagttttt cggtggcgaa cagtacctgc acctgattca
    19621 cacctgcaag ctggcgcacg gcattttcta ccttgcgcgc acaggcggcg cagtccatgc
    19681 cgctgacttt ccagctatag cgggtgccgg agacgttttc agagagcgtt ggcgagctgg
    19741 agcatgcgcc gtcgcagcaa cagtcgttgg cgttctgtac cgtggttagc ggtttgaacg
    19801 cagcaaattg aggggctttc ttgccgtgat tgtcaggtgt cgacatggca tcctccggtt
    19861 aagttttttc tcattaaccg aaggatacac tctggagtcg actccagagt caagttttat
    19921 cagagataca gtgagcggac gatgaggaag tgaccggcaa agtagcaggc cgcagcaatc
    19981 gcgttatccg cacggaagcg tcggcgatag tggctcccca gccagacaaa gttactgata
    20041 aacagcaacg aagcgccaac aaacgcagag agcgccggag cggtcggacg gaagaaccac
    20101 agttcacctg ccagccacac catcaccagc gtcatgccga taaacgtgca gataggccaa
    20161 cgatactctt ccagccgcgt ccagataatc gccagtaaca gcgcacccag aaccagcaac
    20221 accagtggca gaggccagaa gaaagagagc gtcatctgac tggcgaaata gatggtgtac
    20281 agcaggtgcg agaggaaaaa cgcgccgatg gcgtacatca gacgttgacg tggcaacagg
    20341 gttagcgcat cgcccagcag tgaggcgcac agccctgcca gcaccagata gctaatggcg
    20401 tcgaacatcg gcgcttgcca ggccagtaac agcaggagaa gaagggttaa cggtttaaac
    20461 acccagcgtt gccaggttgg cccacgatac gatgcatcca cagatagcca tgcggaaaga
    20521 cagacagcga taaacgacca aagcatctta gttccttgaa ttcgttattt tttcgtaagc
    20581 ggaatgccta cggtcttgct tccagtttag tggcgagggt ggacggatga caacaccata
    20641 actggcaggg tatgcttatt tccgcatttt ccgatgaggg ataaagatga gcaaaccacc
    20701 tcttttcttt attgttatca tttgcttaat tgtcgtcgcc gcatcgtttc gttttatgca
    20761 acagcgacgg gaaaaagctg ataatgatat ggctccgctc cagcaaaagc tggtggtggt
    20821 gagcaacaag cgggaaaaac cgattaacga tcgccgttcg cgccagcagg aagtgactcc
    20881 ggcaggcacc agtatgcgct atgaggcaag cttcaaaccg caaagcggag gaatggagca
    20941 gacgtttagc ctcgacgccc agcagtacca cgccctgaca gtgggcgata aaggtacgct
    21001 gagctataaa ggaacgcgct ttgtcagctt tgtaggcgaa caataaggtt attttttaac
    21061 tttaaatttc ttctgccaga ccagcagttc aaacacgccg aaaaggaaaa tccgcacctt
    21121 ttcggcggtg gtcatctgcg ggccatcttt cggtaaagtg gatttcaata gcgccagttg
    21181 cataccatgc atcagcacgg taaaaatcag cgccacgtta acgaaaatat tcagcgggcg
    21241 cgggaaggga tgcaccagat tgaggattaa aaatccccaa acgcagagca ttaacaaacg
    21301 accaatatta atcagcatca ctttctcctt gtgcttcgcg ttgatacagc cgataagcca
    21361 cctgacccgc cactttttcc cgatgtaatg accagtttgc cggaacagtg ggcagaccgt
    21421 tttcgacttc gctttcgaca taaatcaggg cttcgtcagc cagccagccg ttatcttcca
    21481 gtaaatttat cgtctcttct aacaagccac ggcggaacgg tggatcgaca aacacgatat
    21541 tatgcggtgt acctttttgc gccaggaatg acattgcgtt gctgttcacc acgcgtgcat
    21601 tgcctgcttt tagtgtcgcc agattcttaa ttaactgctg agaaaccgcg cgatccatct
    21661 caatcaacgt tgcccccgca gcatagcgcg acaacgcttc cagccccagc gcgccgcttc
    21721 cggcgaagca atccagacat tgggcgtcaa caatgaccgg agccagccag ttaaacaacg
    21781 tttcgcgtac gcggtcggtg gtggggcgca gacctgggct atcaggaacc gggagtttac
    21841 ggcctcgcca ctgcccgcca ataatgcgga tttggccgct gccggaatga ttcggttttt
    21901 tcatgataaa ttgctcaatc cgccagataa cacatacttg caggcggtga tgtgaattaa
    21961 gttaagtgat agactatttc atcatttttt tagctgctat gtacatagcg ttaacgctgt
    22021 gccatgaagc aacagcgagg agtgtagtcg caaatggcga aagaaaaaaa acgtggcttt
    22081 ttttcctggc tgggctttgg tcaaaaagag cagaccccgg aaaaagagac agaagttcag
    22141 aatgaacaac cggttgtaga agaaatcgtt caggcgcaag agcctgtgaa ggcttcggaa
    22201 caagccgttg aagagcagcc gcaggcgcat actgaagccg aggcggaaac ttttgctgcc
    22261 gacgttgtgg aagtcactga acaggttgct gaaagtgaaa aagcgcagcc tgaagcggaa
    22321 gtcgttgcac agccggaacc ggtcgcagaa gaaacgccgg agccagtggc tatcgaacgt
    22381 gaagagctgc cgttgccaga agacgtcaac gccgaagagg tttcgccaga agagtggcag
    22441 gctgaagcgg aaaccgtaga gattgtcgaa gcggcagaag aagaagcggc taaagaagaa
    22501 atcaccgacg aagagctgga agcacaggcg ctggctgccg aagcggcaga agaggcggtg
    22561 atggtagttc ctccggcaga agaagagcag ccggttgaag aaattgccca ggagcaagaa
    22621 aaaccgacca aagaaggttt cttcgcgcgc ctgaaacgca gcctgttaaa aaccaaagaa
    22681 aatctcggtt ccggatttat cagcctgttc cgcggtaaaa aaatcgacga tgatctgttt
    22741 gaggagctag aagaacagtt gttgatcgcc gatgtgggcg tggaaaccac acgtaaaatt
    22801 atcaccaatc tgacggaagg cgcatcccgc aagcagcttc gtgatgccga ggcgctctat
    22861 ggcctgctga aagaagagat gggcgagatt ctggcgaaag tcgatgagcc gctgaatgtt
    22921 gaaggtaaaa cgccgttcgt gatcctgatg gtgggcgtca acggtgtggg taaaactacg
    22981 acgattggta agctggcacg tcagtttgag cagcagggta aatcggtgat gctggcggcg
    23041 ggcgatactt tccgtgcagc agcggttgaa cagcttcagg tctggggtca gcgcaacaat
    23101 attccggtga ttgcccagca taccggtgcg gattccgcct ctgttatctt cgacgccatt
    23161 caggcagcta aagcgcgtaa tatcgacgtc ctgattgccg ataccgccgg acgcctgcag
    23221 aacaaatcgc acctgatgga agagttgaag aaaatcgtcc gcgtgatgaa gaaactcgac
    23281 gttgaagcgc cgcatgaagt tatgctgact atcgatgcca gtaccgggca gaacgcggta
    23341 agccaggcca aactgttcca tgaagctgtt ggcttaaccg gcatcacgct aacgaaactg
    23401 gacggcacgg cgaaaggcgg ggtaattttc tcggtggctg accagtttgg tatccctatc
    23461 cgctacattg gtgtcggcga acgtattgag gatttgcgtc cgtttaaggc ggacgacttt
    23521 atagaggcac tttttgcccg agaggattaa caatgattcg ctttgaacat gtcagcaagg
    23581 cttatctcgg tgggagacag gcgctgcagg gcgttacgtt ccatatgcag ccgggtgaga
    23641 tggcgtttct gaccggtcat tccggcgcag ggaaaagtac cctcctgaag ctgatctgtg
    23701 ggattgagcg gcccagcgcc gggaaaatct ggtttagcgg ccatgacatc acgcgtctga
    23761 aaaaccgtga agttccgttt ctgcgccgcc agattggcat gattttccag gatcaccatc
    23821 tactgatgga ccgtactgtc tacgataacg tggcgatccc gctgattatc gctggtgcca
    23881 gcggtgacga tattcgtcgc cgggtgtcgg cggcgctgga taaagtcggg ctactggaca
    23941 aagcgaagaa cttccctatt cagctttccg gcggtgaaca acagcgtgtt ggcattgccc
    24001 gcgcggtggt gaacaagccc gcggtactgc tggcggacga accgactggt aacctggacg
    24061 acgcgttgtc ggaagggatt ctacgtctgt ttgaagagtt taaccgcgtt ggggtaaccg
    24121 tattgatggc aacgcacgac atcaacctga tttcgcggcg ttcctatcgc atgctcaccc
    24181 tgagcgatgg tcacttgcat ggaggcgtgg gccatgaata agcgcgatgc aatcaatcat
    24241 attcggcagt ttggcgggcg tctggatcgc ttccgtaaat cggtcggcgg ctcaggcgac
    24301 ggcggtcgta acgcaccgaa acgcgcgaaa tcctcgccaa aaccggtaaa tcgcaaaacc
    24361 aacgttttca acgaacaggt gcgctatgcc ttccacggcg cattgcagga tctgaaaagc
    24421 aaaccgttcg ccacgttttt aacggtgatg gttatcgcca tctccctgac gctgcccagc
    24481 gtctgttata tggtgtacaa aaacgttaat caggcggcga cgcagtatta tccgtcaccg
    24541 caaatcactg tttacttgca aaaaacgctg gacgatgacg ccgctgcggg cgtggtggca
    24601 cagttgcagg ccgagcaagg cgtggagaaa gtgaactatc tttctcgtga agacgcactg
    24661 ggcgagttcc gtaactggtc tggttttggt ggtgcgctgg atatgctgga agaaaacccg
    24721 cttccggcag tggcggtggt gatcccgaaa ctcgattttc aggggacgga atcactcaat
    24781 acgctgcgtg atcgtatcac ccagattaac ggcattgacg aagtgcggat ggatgacagc
    24841 tggtttgccc gtctggcggc gttgaccggg ctggtcgggc gcgtttccgc gatgatcggc
    24901 gtgttgatgg tggcagcagt gttcctcgtc atcggcaaca gtgtgcgtct gagcatcttt
    24961 gctcgccgtg actccattaa cgtgcagaaa ctgattggtg cgacggatgg tttcatcctg
    25021 cgcccgttcc tgtacggagg cgcattgctg ggattttctg gcgcattgct gtcattaatt
    25081 ttgtcagaaa ttctggtact gcgtttgtca tcggcggttg cagaagtggc acaggttttc
    25141 ggaacgaagt ttgatatcaa tggtttatcg ttcgatgaat gcctgctatt gctgctggta
    25201 tgctcgatga ttggctgggt ggcagcgtgg cttgccacgg tacaacattt acgccacttt
    25261 acgcctgaat aataaaagcg tgttatactc tttccctgca atgggttccg tagcagggaa
    25321 agagatcccg ttgtctcttc ccggtatttc atctctatgt cacattttgt gcgtaattta
    25381 ttcacaagct tgcattgaac ttgtggataa aatcacggtc tgataaaaca gtgaatgata
    25441 acctcgttgc tcttaagctc tggcacagtt gttgctacca ctgaagcgcc agaagatatc
    25501 gattgagagg atttgaatga ctgacaaaat gcaaagttta gctttagccc cagttggcaa
    25561 cctggattcc tacatccggg cagctaacgc gtggccgatg ttgtcggctg acgaggagcg
    25621 ggcgctggct gaaaagctgc attaccatgg cgatctggaa gcagctaaaa cgctgatcct
    25681 gtctcacctg cggtttgttg ttcatattgc tcgtaattat gcgggctatg gcctgccaca
    25741 ggcggatttg attcaggaag gtaacatcgg cctgatgaaa gcagtgcgcc gtttcaaccc
    25801 ggaagtgggt gtgcgcctgg tctccttcgc cgttcactgg atcaaagcag agatccacga
    25861 atacgttctg cgtaactggc gtatcgtcaa agttgcgacc accaaagcgc agcgcaaact
    25921 gttcttcaac ctgcgtaaaa ctaagcagcg tctgggctgg tttaaccagg atgaagtcga
    25981 aatggtggcc cgtgaactgg gcgtaaccag caaagacgtt cgtgagatgg aatcacgtat
    26041 ggcggcacag gacatgacct ttgacctgtc ttccgacgac gattccgaca gccagccgat
    26101 ggcaccggtg ctctatctgc aggataaatc atctaacttt gccgacggca tcgaagatga
    26161 taactgggaa gagcaggcgg caaaccgtct gaccgacgcg atgcaaggtc tggacgaacg
    26221 cagccaggac atcatccgcg cgcgctggct ggacgaagac aacaagtcca cgttgcagga
    26281 actggctgac cgttacggtg tttccgctga acgtgtgcgc caactggaaa agaacgcgat
    26341 gaaaaaactt cgcgccgcta ttgaagcgta atttccgcta ttaagcagag aaccctggat
    26401 gagagtccgg ggtttttgtt ttttgggcct ctacaataat caattccccc tccggcaaaa
    26461 cgtcaatccc cacgcagatt gttaataaac tgtcaaaata gctattccaa tatcataaaa
    26521 atggggtatg ttttagcaga gtatgctgct aaagcacggg tagtcatgca taaaacgaaa
    26581 taaagtgctg aatcacaaca ccacaacaca cgtaataacc agaagaatgg ggattctcag
    26641 gatgaacata aagggtaaag cgttactggc aggatgtatc gcgctggcat tcagcaatat
    26701 ggctctggca gaagatatta aagtcgcggt cgtgggcgca atgtccggtc cggttgcgca
    26761 gtacggtgac caggagttta ccggcgcaga gcaggcggtt gcggatatca acgctaaagg
    26821 cggcattaaa ggcaacaaac tgcaaatcgt aaaatatgac gatgcctgtg acccgaaaca
    26881 ggcggttgcg gtggcgaaca aagtcgttaa cgacggcatt aaatatgtga ttggtcacct
    26941 ctgttcttca tcaacgcagc ctgcgtctga catctacgaa gacgaaggca ttttaatgat
    27001 caccccagcg gcaaccgcgc cggagctgac cgcccgtggc tatcagctga tcctgcgcac
    27061 caccggcctg gactccgacc aggggccgac ggcggcgaaa tatattcttg agaaagtgaa
    27121 accgcagcgt attgctatcg ttcacgacaa acagcaatac ggcgaaggtc tggcgcgagc
    27181 ggtgcaggac ggcctgaaga aaggcaatgc taacgtggtg ttctttgatg gtatcaccgc
    27241 cggggaaaaa gatttctcaa cgctggtggc ccgtctgaaa aaagagaata tcgacttcgt
    27301 ttactacggc ggttatcacc cggaaatggg gcaaatcctg cgtcaggcac gcgcggcagg
    27361 gctgaaaact cagtttatgg ggccggaagg tgtggctaac gtttcgctgt ctaacattgc
    27421 gggcgaatca gcggaagggc tgctggtgac caagccgaag aactacgatc aggttccggc
    27481 gaacaaaccc attgttgacg ccatcaaagc gaaaaaacag gacccaagcg gcgcattcgt
    27541 ttggaccacc tacgccgcgc tgcaatcttt gcaggcgggc ctgaatcagt ctgacgatcc
    27601 ggctgagatc gccaaatacc tgaaagcgaa ctccgtggat accgtgatgg gtccgctgac
    27661 gtgggatgag aaaggtgatc tgaaaggctt tgagttcggc gtatttgact ggcacgccaa
    27721 cggcacggcg accgatgcga agtaatcatt aatcggcaac tttgggttgc cgccaaattg
    27781 ctaatatcga gtacgttgct tcatgccgga tgcggcgtaa acgccttatc cggcctacaa
    27841 gatccaaaga aatcagtaaa ttgcaacaca cattgtaggc ctgataagcg tagcgcatca
    27901 ggcaatttct cgttggtcat cacttttgaa atcaacttga cgattaacgc ttctcccagc
    27961 cgccctgttg cgccgtaaac cccagcgcct gcataaacgc cgtcatcaca ccgcgatctt
    28021 ccacgcctgc atccgccatc caccagcatg aaacggcagg attgttacgc aaaacctctt
    28081 ccagcagata ttgccccaca ccgcgacggc gggtgacttc ccgcacgcgc agggaatcca
    28141 gtgctccctc ggtgccgctt aaggttaccc gcacggccgc tagcaggcgc tcgttaaaac
    28201 gcgcggcgta gatgcggtgg ttatcgtcaa cctgtaaaga ggaaggggaa tactccggcc
    28261 agatcttttg caggtcaatc cggtcctggt cgctaaagtt ttctaatcga atgatggtca
    28321 gcttcatggg gaacccgtgt aaatcacaaa agtggaacca gtgtagcgaa ataatttaat
    28381 cggaggcttt ctctttttta tttcttttgg caggtgatt
//
LOCUS       DAZFVO020000055        28405 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFVO020000055 DAZFVO020000000
VERSION     DAZFVO020000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 28405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.055"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            22..96
                     /gene="tnaC"
                     /locus_tag="ACHKNB_RS10895"
                     /old_locus_tag="pgaptmp_002179"
     CDS             22..96
                     /gene="tnaC"
                     /locus_tag="ACHKNB_RS10895"
                     /old_locus_tag="pgaptmp_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HGU4195682.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            317..1732
                     /gene="tnaA"
                     /locus_tag="ACHKNB_RS10900"
                     /old_locus_tag="pgaptmp_002180"
     CDS             317..1732
                     /gene="tnaA"
                     /locus_tag="ACHKNB_RS10900"
                     /old_locus_tag="pgaptmp_002180"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HGU4195683.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1823..3070
                     /gene="tnaB"
                     /locus_tag="ACHKNB_RS10905"
                     /old_locus_tag="pgaptmp_002181"
     CDS             1823..3070
                     /gene="tnaB"
                     /locus_tag="ACHKNB_RS10905"
                     /old_locus_tag="pgaptmp_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HGU4195684.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3202..4377
                     /gene="mdtL"
                     /locus_tag="ACHKNB_RS10910"
                     /old_locus_tag="pgaptmp_002182"
     CDS             3202..4377
                     /gene="mdtL"
                     /locus_tag="ACHKNB_RS10910"
                     /old_locus_tag="pgaptmp_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HGU4195685.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4352..5311
                     /gene="yidZ"
                     /locus_tag="ACHKNB_RS10915"
                     /old_locus_tag="pgaptmp_002183"
     CDS             4352..5311
                     /gene="yidZ"
                     /locus_tag="ACHKNB_RS10915"
                     /old_locus_tag="pgaptmp_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HGU4195686.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5468..6217
                     /gene="yieE"
                     /locus_tag="ACHKNB_RS10920"
                     /old_locus_tag="pgaptmp_002184"
     CDS             5468..6217
                     /gene="yieE"
                     /locus_tag="ACHKNB_RS10920"
                     /old_locus_tag="pgaptmp_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HGU4195687.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6239..6805
                     /gene="yieF"
                     /locus_tag="ACHKNB_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /gene_synonym="chrR"
     CDS             6239..6805
                     /gene="yieF"
                     /locus_tag="ACHKNB_RS10925"
                     /old_locus_tag="pgaptmp_002185"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HGU4195688.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6859..8196)
                     /gene="adeP"
                     /locus_tag="ACHKNB_RS10930"
                     /old_locus_tag="pgaptmp_002186"
     CDS             complement(6859..8196)
                     /gene="adeP"
                     /locus_tag="ACHKNB_RS10930"
                     /old_locus_tag="pgaptmp_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HGU4195689.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8363..9028
                     /gene="yieH"
                     /locus_tag="ACHKNB_RS10935"
                     /old_locus_tag="pgaptmp_002187"
     CDS             8363..9028
                     /gene="yieH"
                     /locus_tag="ACHKNB_RS10935"
                     /old_locus_tag="pgaptmp_002187"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HGU4195690.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9095..9568
                     /locus_tag="ACHKNB_RS10940"
                     /old_locus_tag="pgaptmp_002188"
     CDS             9095..9568
                     /locus_tag="ACHKNB_RS10940"
                     /old_locus_tag="pgaptmp_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195691.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9617..10204
                     /gene="cbrC"
                     /locus_tag="ACHKNB_RS10945"
                     /old_locus_tag="pgaptmp_002189"
     CDS             9617..10204
                     /gene="cbrC"
                     /locus_tag="ACHKNB_RS10945"
                     /old_locus_tag="pgaptmp_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HGU4195692.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10266..10988)
                     /gene="yieK"
                     /locus_tag="ACHKNB_RS10950"
                     /old_locus_tag="pgaptmp_002190"
     CDS             complement(10266..10988)
                     /gene="yieK"
                     /locus_tag="ACHKNB_RS10950"
                     /old_locus_tag="pgaptmp_002190"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGU4195693.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11003..12172)
                     /gene="yieL"
                     /locus_tag="ACHKNB_RS10955"
                     /old_locus_tag="pgaptmp_002191"
     CDS             complement(11003..12172)
                     /gene="yieL"
                     /locus_tag="ACHKNB_RS10955"
                     /old_locus_tag="pgaptmp_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4195694.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12199..13815)
                     /gene="bglH"
                     /locus_tag="ACHKNB_RS10960"
                     /old_locus_tag="pgaptmp_002192"
     CDS             complement(12199..13815)
                     /gene="bglH"
                     /locus_tag="ACHKNB_RS10960"
                     /old_locus_tag="pgaptmp_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HGU4195695.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13901..15295)
                     /gene="bglB"
                     /locus_tag="ACHKNB_RS10965"
                     /old_locus_tag="pgaptmp_002193"
     CDS             complement(13901..15295)
                     /gene="bglB"
                     /locus_tag="ACHKNB_RS10965"
                     /old_locus_tag="pgaptmp_002193"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HGU4195696.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15314..17191)
                     /gene="bglF"
                     /locus_tag="ACHKNB_RS10970"
                     /old_locus_tag="pgaptmp_002194"
     CDS             complement(15314..17191)
                     /gene="bglF"
                     /locus_tag="ACHKNB_RS10970"
                     /old_locus_tag="pgaptmp_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HGU4195697.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17324..18160)
                     /gene="bglG"
                     /locus_tag="ACHKNB_RS10975"
                     /old_locus_tag="pgaptmp_002195"
     CDS             complement(17324..18160)
                     /gene="bglG"
                     /locus_tag="ACHKNB_RS10975"
                     /old_locus_tag="pgaptmp_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HGU4195698.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18446..19171)
                     /gene="phoU"
                     /locus_tag="ACHKNB_RS10980"
                     /old_locus_tag="pgaptmp_002196"
     CDS             complement(18446..19171)
                     /gene="phoU"
                     /locus_tag="ACHKNB_RS10980"
                     /old_locus_tag="pgaptmp_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGU4195699.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19186..19959)
                     /gene="pstB"
                     /locus_tag="ACHKNB_RS10985"
                     /old_locus_tag="pgaptmp_002197"
     CDS             complement(19186..19959)
                     /gene="pstB"
                     /locus_tag="ACHKNB_RS10985"
                     /old_locus_tag="pgaptmp_002197"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGU4195700.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20050..20940)
                     /gene="pstA"
                     /locus_tag="ACHKNB_RS10990"
                     /old_locus_tag="pgaptmp_002198"
     CDS             complement(20050..20940)
                     /gene="pstA"
                     /locus_tag="ACHKNB_RS10990"
                     /old_locus_tag="pgaptmp_002198"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGU4195701.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20940..21899)
                     /gene="pstC"
                     /locus_tag="ACHKNB_RS10995"
                     /old_locus_tag="pgaptmp_002199"
     CDS             complement(20940..21899)
                     /gene="pstC"
                     /locus_tag="ACHKNB_RS10995"
                     /old_locus_tag="pgaptmp_002199"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HGU4195702.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21986..23026)
                     /gene="pstS"
                     /locus_tag="ACHKNB_RS11000"
                     /old_locus_tag="pgaptmp_002200"
     CDS             complement(21986..23026)
                     /gene="pstS"
                     /locus_tag="ACHKNB_RS11000"
                     /old_locus_tag="pgaptmp_002200"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGU4195703.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23274..24347)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKNB_RS11005"
                     /old_locus_tag="pgaptmp_002201"
     CDS             complement(23274..24347)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKNB_RS11005"
                     /old_locus_tag="pgaptmp_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HGU4195704.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(24358..26880)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKNB_RS11010"
                     /old_locus_tag="pgaptmp_002202"
     CDS             complement(24358..26880)
                     /gene="lpfC-O113"
                     /locus_tag="ACHKNB_RS11010"
                     /old_locus_tag="pgaptmp_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HGU4195705.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26905..27636)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKNB_RS11015"
                     /old_locus_tag="pgaptmp_002203"
     CDS             complement(26905..27636)
                     /gene="lpfB-O113"
                     /locus_tag="ACHKNB_RS11015"
                     /old_locus_tag="pgaptmp_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HGU4195706.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(27684..28256)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKNB_RS11020"
                     /old_locus_tag="pgaptmp_002204"
     CDS             complement(27684..28256)
                     /gene="lpfA-O113"
                     /locus_tag="ACHKNB_RS11020"
                     /old_locus_tag="pgaptmp_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HGU4195707.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDVVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASLQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         7275 a         7102 c         6605 g         7423 t
ORIGIN      
        1 tctttcgagg ataagtgcat tatgaatatc ttacatatat gtgtgacctc aaaatggttc
       61 aatatcgaca ataagattgt cgatcaccgc ccttaattta cccttctgta gccatcacca
      121 gagccaaacc gattagattc aatgtgaacg attagtttgc tatatcttaa ttttgccttt
      181 tgcaaaggcc atctctcgtt tatttactta ttttagtaaa tgatggtgct tgtatatata
      241 catggcgaat taatggatat tgcagatgta atattcacag ggatcactgt aattaaaata
      301 aatgaaggat tatgtaatgg aaaactttaa acatctccct gaaccgttcc gcattcgtgt
      361 tattgagcca gtaaaacgta ctactcgcgc ttatcgtgaa gaagcaatta ttaaatccgg
      421 tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc gatttactga ccgacagcgg
      481 caccggggcg gtaacccaaa gtatgcaggc agcgatgatg cgcggcgacg aagcctacag
      541 cggcagccgc agctactatg cgttagccga gtcagtgaaa aatatctttg gttatcaata
      601 caccattccg actcaccagg gccgtggcgc agagcaaatt tatattccgg tactgattaa
      661 aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg gtggcattct ctaactattt
      721 ctttgatacc acgcagggcc atagccagat taacggctgt accgtgcgta acgtctatat
      781 caaagaagcc ttcgatactg gcgtgcgtta cgactttaaa ggtaactttg accttgaggg
      841 attagaacgc ggtattgaag aagttggccc gaataacgtg ccgtatatcg ttgcaaccat
      901 caccagtaac tctgcaggtg gtcagccggt ttcactggca aacttaaaag cgatgtacag
      961 catcgcgaag aaatacgata ttccggtggt aatggactcc gcacgctttg ctgaaaacgc
     1021 ctatttcatc aagcagcgtg aagcagaata caaagactgg accatcgagc agatcacccg
     1081 cgaaacctac aaatatgccg atatgctggc gatgtccgcc aagaaagatg cgatggtacc
     1141 gatgggcggc ttgctgtgca tgaaagacga cagcttcttt gatgtgtaca ccgagtgcag
     1201 aaccctttgc gtggtgcagg aagggttccc gacatatggc ggcctggaag gcggcgcgat
     1261 ggagcgtctg gcggtaggtc tgtatgacgg catgaatctc gactggctgg cttatcgtat
     1321 cgcgcaggta cagtatctgg tcgatggtct ggaagagatt ggcgttgtct gccagcaggc
     1381 gggcggtcac gcggcattcg ttgatgccgg taaactgctg ccgcatatcc cggcagacca
     1441 gttcccggca caggcgctgg cgtgcgagct gtataaagtc gccggtatcc gtgcggtaga
     1501 aattggctct ttcctgttag gccgcgatcc gaaaaccggt aaacaactgc catgcccggc
     1561 tgaactgctg cgtttaacca ttccgcgcgc aacatatact caaacacata tggacttcat
     1621 tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat attaaaggat taacctttac
     1681 ctacgaacca aaagtattgc gtcacttcac cgcaaaactg aaagaagttt aattaatact
     1741 acagagtggc tataaggatg ttagccactc tcttacccta catcctcaat aacaaaaata
     1801 gccttcctct aaaggtggca tcatgactga tcaagctgaa aaaaagcact ctgcattttg
     1861 gggtgttatg gttatagcag gtacagtaat tggtggaggt atgtttgctt tacctgttga
     1921 tcttgccggt gcctggtttt tctggggtgc ctttatcctt atcattgcct ggttttcaat
     1981 gcttcattcc gggttattgt tattagaagc aaatttaaat tatcctgtcg gctccagttt
     2041 taacaccatc accaaagatt taatcggtaa cacctggaac attatcagcg gtattaccgt
     2101 tgccttcgtt ctctatatcc tcacttatgc ctatatctct gctaatggtg cgatcattag
     2161 tgaaacgata tcaatgaatt tgggctatca cgctaatcca cgtattgtcg ggatctgcac
     2221 agccattttc gttgccagcg tattgtggat aagttcgtta gccgccagtc gcattacctc
     2281 attgttcctc gggctgaaga ttatctcctt tgtgatcgtg tttggttctt tcttcttcca
     2341 ggtcgattac tccatcctgc gcgatgccac cagcaccact gcgggaacgt cttacttccc
     2401 gtatatcttt atggctttgc cggtgtgtct ggcgtcattt ggtttccacg gcaatattcc
     2461 cagcctgatt atttgctatg gaaaacgcaa agataagtta atcaaaagcg tggtatttgg
     2521 ttcgctgctg gcgctggtga tttatctctt ctggctctat tgcacgatgg ggaatattcc
     2581 gcgcgaaagc tttaaggcga taatctcctc aggcggcaac gttgattcgc tggtgaaatc
     2641 gttcctcggc accaaacagc acggcattat cgagttttgc ctgctggtgt tctctaactt
     2701 agctgttgcc agttcgttct ttggtgtcac gctggggttg ttcgattatc tggcggacct
     2761 gtttaagatt gataactccc acggcgggcg tttcaaaacc gtgctgttaa ccttcctgcc
     2821 acctgcgttg ttgtatctga tcttcccgaa cggctttatt tacgggatcg gcggtgccgg
     2881 gctgtgcgcc accatctggg cggtcattat tcccgcagtg cttgcaatca aagctcgcaa
     2941 gaagtttccc aatcagatgt tcacggtctg gggcggcaat cttattccgg cgattgtcat
     3001 tctctttggt ataaccgtga ttttgtgctg gttcggcaac gtctttaacg tgttacctaa
     3061 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
     3121 cttatgtcca aatggcaact cgcctgattc tccttcacca cgtatgcttt gcgtcacctt
     3181 actattagga cgctttagcc catgtcccgc tttttaattt gtagttttgc cctggtttta
     3241 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgacgatctc
     3301 aatgccagcg aagcgcagtt gcatattgcg ttctccgttt atctggcggg catggcagct
     3361 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagcctgt cgccatcccc
     3421 ggcgcggcgc tatttattat cgcttcggtg ttctgttcac tggctgaaac cagcacgtta
     3481 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
     3541 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
     3601 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
     3661 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
     3721 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
     3781 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
     3841 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
     3901 ggttttgagc gcggagaata cgccaccatt atggcgttga ctgctggcgt cagcatgacc
     3961 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
     4021 tcgcaggtgt tattccttgc agcggggatc acccttgccg tttcaccttc ccatgcggtt
     4081 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     4141 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     4201 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tatcggcgca
     4261 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     4321 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     4381 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     4441 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     4501 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     4561 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     4621 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     4681 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     4741 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     4801 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     4861 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     4921 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     4981 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     5041 gattgctatg agcctgccgg aatttgagca gtcactgttt atggcagcgc aacccgacaa
     5101 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     5161 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     5221 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     5281 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     5341 acaataaaat gtaaaaaagt tgtaataagc ttgtctgaat cgaactttta gccgctttag
     5401 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     5461 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     5521 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     5581 ttttctggcg tctagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     5641 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     5701 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     5761 atgtggtctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     5821 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     5881 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     5941 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     6001 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     6061 ggtcacgccc acgattgaaa agctcagtgt ctgggagttc gatggcaaac acggctggaa
     6121 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     6181 ccagctctct accgtgaagg ctttttcgcc aaattgatag acaaccacag gagtcatcat
     6241 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     6301 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     6361 ttccattgcc gacattccct tgtatgacgc tgacgtacag caggaagaag gttttccagc
     6421 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     6481 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     6541 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     6601 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     6661 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg
     6721 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     6781 gtttattcag cgagttaaga tctaaataaa aaacccgcca gcaatcatgc atggcgggtt
     6841 tttaacgcgc tatcgatttt agtgagcgtc gataaagaca atcttcagga taaacagcag
     6901 cgcaacgatg attacgcacg ggcttagatc acgcagacgt ccggtaccga ttttcatcac
     6961 gcagtaggag ataaagccca gcgcgatacc ttcggtaatc gagaagctga acggcatcat
     7021 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgtgccag
     7081 actggaagtc atcagcacgc caacgtaaat cagcgcacca gctgcagcgt agcctggcac
     7141 catccccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     7201 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     7261 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     7321 cttcatgcgc gggaatttac ccttctcatc cgccaggcct gctttatcgg tcacgccaat
     7381 cagcgtaccg gaggagtcaa acaggttgac cagcatgaaa gagaaaatca cccctgccag
     7441 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     7501 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     7561 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa
     7621 gaagccgagg atccccagca gcacgctgtg agaagtcagg ttaccgatgc tgaccagcgt
     7681 ttccgggtta gcgacaatca cacctgcatt cttcagtccc atcataccaa tgaacagacc
     7741 gataccgctg gtgatgccca cacgcagact taccggaatg ttggcaatca tccagtagcg
     7801 aacgcggaaa atcgtcagta acagcagacc aatcgcaccc cagaagattg cgcccatccc
     7861 gacttgccac ggtaagccca tcgcctgtac aacgacaaaa gcgaagaacg cattcaggcc
     7921 catagcgggt gccagtgcaa ctggcaggtt agcaaacagt cccatcataa tactgccgaa
     7981 tgcagcgatc agacaggtag tgacgaagac ggcgctggta tccatgccag caacgccaag
     8041 aatttgcggg ttaacaaaaa cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat
     8101 cacttcggtc cgtgccgtcg tgccatgttc gcgcagttta aacacgcgtt ccagcatccc
     8161 ctggccagaa gcctgggttg tgtgttgttg actcattatc tatttccgaa caaggaggga
     8221 aaatccgtcg ctatcgtata ccaaaatgag acaatatgga cggctgaaga taactttttt
     8281 tcattgtatt tgcttatacg gcaacgattg cgtcatgcga attggcatga cgaaaacgtt
     8341 aaactaacga taaaggaaag acatgtccca gatagaagcg gtatttttcg actgcgacgg
     8401 tacgctggtc gacagtgaag tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt
     8461 tggtattacg ctcgatcctg aagaggtatt caaacgtttc aaaggtgtaa aactgtacga
     8521 aattatcgat attgtttccc ttgaacatgg tgttacgtta gcgaaaacag aagctgaaca
     8581 cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa ctggaagcca tcgaaggggc
     8641 tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg gcccaaataa
     8701 caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg ataaactgtt
     8761 cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc atgcggcaaa
     8821 agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg ccggtgcgca
     8881 atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc acaataagcc
     8941 gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg aactgtggaa
     9001 agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta ccccgcttaa
     9061 attggcgctc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc gggtaataca
     9121 tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc ttgtcctcta
     9181 catattcttc gcaaaagaac cgctggttct tttgcgaaga atacaggtac ttccattgtt
     9241 tctcttattg tcgattacta cgggagttat tcctgcattg ttaaccggcg tgatggtcgc
     9301 ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg gtggcatagg
     9361 tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg gcatggcttc
     9421 ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca tcattcctgc
     9481 attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat tggatatttc
     9541 atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc tactcaaaag
     9601 cagacaggat gttactatga ctcaaaatat caggccgtta ccccaattca aatatcatcc
     9661 caagccactg gaaacaggcg catttgaaca ggataaaacc gtagagtgcg attgctgtga
     9721 acaacagacg tcagtttatt actcgggtcc cttttattgc gttgatgaag ttgaacatct
     9781 ctgtccgtgg tgtattgcgg acggttctgc tgctgaaaag tttgcaggta gttttcagga
     9841 tgatgccagc atagaaggtg ttgaatttga gtatgatgaa gaggacgaat ttgccggtat
     9901 taagaacaca tatcctgatg aaatgctgaa agagttggtt gaacgcacgc caggttatca
     9961 tggatggcaa caggaattct ggcttgcgca ttgtggcgat ttctgtgctt ttatcggcta
    10021 tgtgggctgg aatgatataa aagatcgcct cgatgaattt gccaaccttg aagaagattg
    10081 tgagaatttc ggtattagaa attctgatct agctaaatgc ctgcaaaagg gtggtgattg
    10141 tcagggttat ctcttccgct gtctccactg cggcaagctg agactgtggg gtgatttttc
    10201 gtagttattt aaataatgag aacaggccgg agcgtaattc atacatccgg ccttatttct
    10261 tcagcttaac cgagcgctaa ttctgcggca gcggctttat cagcgattac catcagcgaa
    10321 ggatgcagct gcaacacgga agctggaaca tcttcggtaa ccggaccttg cagaacattt
    10381 ttcagtgcct gcgctttacc tgcgccactg acaattatca gcaagttttt cgccgccatg
    10441 atgcttttcg gccccatcgt cacgtagctg tcaggcacta acgaaaagtc gccgcccaac
    10501 tcaccatgcg ccacaatatc aaccatctct ccctggatcg ggaactccac ggtttgctca
    10561 tggaaatggg tcgtattcgg caaattgcca caaaaatgac catctgcacc taatcccagc
    10621 accaccaaat ccaggccgcc ttcacgcgcc agtttctgat catgctcgcg gtagttatca
    10681 atagtgagct tctggatatt ctcttctttg atccccgcag gggtgaaaaa cagattacgc
    10741 agattggtaa tcgttacacc ttctccctct ttgccgcgaa agggaatttc atcgaaatta
    10801 tagaaatagc agttatcgta ccagggctta cctttaacca gggtggtgag gtattcatac
    10861 atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa cacgacgcgt cttcgacata
    10921 taaccaagca gatggtgtgc cgcgacacgg ctcatttcct ggtaatcttc ggtaatgatt
    10981 aatttcatct tagtgcctta ttttatttaa ataatttctg tacgaaggct gcataggcag
    11041 gccgccagac atccatttca tggttcaggc cgggatattc ctggtaatca aacttaattt
    11101 ttttctgctc aagctcagtt ttcagcccgg cgatatcctt gccggttacg acatctttat
    11161 caccaacgac cacagtaaaa ttacgtagtt gctggttaat agctgccgga tcgttcagcc
    11221 gggccgcgac accttcatcc ggtacggttg tcgtggtaac accactgaat gtggccagcc
    11281 agccaaagct ttccagatga ttcattcccg ataccagcgc ctgataccca ccttgtgaaa
    11341 gccctgccag tgcgcgacca tcggcatctt tactgacgtt aaaacgcttg ctaatcagcg
    11401 ggataatatc gttcatcagt tcgcgatctg ccgctttagc attcagcgga taaaagactt
    11461 tacgtctttc ctgaggcacg aaatcttcgg gaataatgcc cttcgcatcg gtttcggtat
    11521 ccgggatcac caccagcatc ggtttaattt tcccttcagc cagcaggtta tccatgattt
    11581 gcgggatacg cccctgatcg atagcggaac gtccggtatc accaaaaccg tgatagaaat
    11641 agagcactgg caaaggctcg cccatgccgg tgtatcctgg cggggtccag acatacatct
    11701 gacgttcaga ttgcaatgcg ttggagtggt aagttatggc gatcaaatca ccatgcgcaa
    11761 cagaacgcgt atccagataa ctgcctggca ccagaatcat actggagtta acctggcgct
    11821 gaggcttagt cattgcggtg cctgtatcaa tgctgcgtac accatcaaca ttgaaaaaat
    11881 actcgtacag gttgcctttc aggataggtg tgcgccacga ccagactcct gcttcgtctt
    11941 tggtcatcgg gtgaatattg tccagaaccg gaacacccac cactacagag acattttttg
    12001 ccccaggggc aaagtagcga aaggtcacgc tgttatcggc attgacctga gtcacatact
    12061 gtttcaccgg gatagtgggt gccgggctgg ctggcatatc cgctgctatg gcccactgag
    12121 cagaaatacc gctggcaata gccagcgtta aagcagcaat ttttatattc atcgtaatat
    12181 tcctttcatt aagttccatt accaccagat ttcagcctgg gcaccgacag caaactggtc
    12241 gtctttattg tcttcgaagg tgaagccatc cagttcgttt tccagggctt tgatatacgt
    12301 ggcgtagaaa cgaatttccg gacgcgaggt caacatactg gtattgactt taaaggtatg
    12361 gaagagcgtg gttttataac cagactcatt aaatttatta ctattcgcat ctttgttttg
    12421 ctgggtgaaa tagcccagtt caacacctgt ctggttatat tggtcccaaa tataggccgg
    12481 gcgaacaacc gcacgaatag attcgaaatc agagtgtgcg cctgtttcgt agctataaat
    12541 atcgttaccg aaggagtaaa caatcgcgtt agccacaatg aaatgatcgc cgatataggc
    12601 ttcgccctgt gaagtcagac gaactgctgt tccgccggtg tgatcaccat aataacgacc
    12661 attaaaggtg gtaaatggac ttgcgccagc ataacggcca aagttactgg cgatagagtt
    12721 attcgcaacc aggaaggaga attcgttgaa gccaccttta tcaaatttct gcgttaaaga
    12781 tgtgccaaac atccaggtat ctttccagtc ataatagccg ttattatcct gattatcttt
    12841 ttccgatgcg ctttcgtttg ccgtgacata acgaccactc accattaagg tcgctttatc
    12901 ccataacggg atatctttat agcgtaaatc aatagtattg gtattaatct gctgtttgtt
    12961 ttgcaggctg cgatcgtaat catcgacatc ttcgcgaacc agcgcgatat caattttacc
    13021 cggaccgact ttccagtttt ccagacctac acccgctgcg gcatcagtac gctgcgtttt
    13081 ccagtccagc atctggattt caattttcgg cgcaccgtgt ttacccaccc agaaatcagc
    13141 ctctggcgca aagggcagga aacctttggt ggtaacgtac atatcggaga actgcataaa
    13201 gttctcgcca ccagcgttat cgccaaacca gccggtagag tactgctgac caacgttacc
    13261 atccatcatc acaacggcat caacccgttt gccgttttcg ttgtagacac gttgttttaa
    13321 ctgcaaatca aaccagccgg agtattcgtt accaaagcgg cccagagaac caatcgccca
    13381 tgatttaggt gaaccatgag aggcggtccc ccaaccagaa cggtaatagc cgttgtaact
    13441 aaagccaatt tcatctttca caaacttact gaaatctttc agagtcatgg aagaataaat
    13501 cgagcctgtc tcactggcat ttttctcttc atttttgacc agtacagcat caggttttgc
    13561 ggcactactg gtcgggaacg gattggcggc atacccttga tcattcgtac ttacgctacg
    13621 attcaccgtc gctggcgtat attttttctt ctcttcatct ttatactttt tgagttcgct
    13681 ttgcgtttct cttaacgctt tttctaataa ctcaaggcga tgttccaccg ttaatgattc
    13741 agccaatgat tgtgcagaaa aggctaacgg tgccattaat aagatggcag aggtaataag
    13801 atttcgtcta aacataatat ccctttatgg tgcaaagaaa gaattaacgt atcgcatcca
    13861 gactgttctg aatgcgacga taattaaggt gctttattgg ttattttttt aatgacagcc
    13921 cccgcgtttt gatcacttct gcataccatc cgaagctttt cttacgcgtt cttgtgaggc
    13981 ttccttcgcc attatcatca cgatccacat aaataaagcc gtagcgctta gacatttgtg
    14041 aatgagatgc actgactaaa tcaattggcc cccaactggt gtaccccata atatccacac
    14101 catcggcaat cgcttcattt acctgtacca ggtgatcgtt taaataggca attcgataat
    14161 cgtcctgtat cgaaccatcc gcttcaacgc tgtcttttgc gcctaatccg ttctcgacaa
    14221 taaataacgg tttttgataa cgatcccaaa gcgtatttaa caaaacccgt aatccaaccg
    14281 gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc atattcagta
    14341 tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca gtcatgtaat
    14401 aactaaaaga gatgaaatct acggtatgtt ttaaatcttc tgcgtcactt tcagtcatct
    14461 caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatac tgaccacgcg
    14521 cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctcc aacatatcct
    14581 gtggctggca ggtgaggggg taaaccagcc cgccgagaag catattgccg attttcgctt
    14641 cggggatcag gctatgacag gctttaactg cccgcgcact ggcaaccagt tgatgatgga
    14701 tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc gtgaatggcg
    14761 cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta tgttggtagc
    14821 gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga ttagcccaac
    14881 cgccgtagtt tttcaccagc ccatatggca tttcgtaatg ggataacgtt accagcggct
    14941 tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac cccgcttcat
    15001 tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac
    15061 aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtga taaaaatcga
    15121 tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt cccattacgc
    15181 catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag gcaccttcca
    15241 cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct ttcataatta
    15301 actcctttta tcgttagcga atgatggata acagcggctc accagcgctt atctgcgccg
    15361 tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc ggcgtcgtca
    15421 gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga tcgcctgtat
    15481 tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt tttacggtgt
    15541 cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca atagcgtgta
    15601 atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca ccaaccgagg
    15661 gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa
    15721 tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca ggtggtgtaa
    15781 taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta gcggtgatga
    15841 aatgaagcat caccgtaccg acaaatgcgc aaccgatggc aatgacaccg ccaataacgc
    15901 tggcccagac ggtgaaatca attcccgttg acgggatggt ttgcatgaag gtgaaaatac
    15961 ttggcaaacc aaaggagtac actttcgttt gcgcgtagcc aatgatggtg gcccccaaag
    16021 ccccactgat acaggcgata acaaaggggt acttacgcgg cagattaacg ccatataccg
    16081 ctggttcggt gataccaaac aggcccgtca acgccgctga tcccgccacc acttttttct
    16141 gcgcatcacg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc ataatggcgg
    16201 gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg atacacagcg
    16261 gcaccaggcc ccagtgcagt ccgaacatga cgaagatttg ccagaagccg cccattaccg
    16321 cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca atcagttcgc
    16381 ttatccaggt tgatagcggc cccaccagca gaaaggtgac gggtgtgata accatcagac
    16441 atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta agtcggcgtt
    16501 ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca
    16561 acaatgtgac cggaataccc aggaaatcca gccccagctc atccgctttt tgcccgttct
    16621 cgaaagcagt cagaattaat ggatgcacta acgctccacc aatcaccatg gcagtaaatg
    16681 gattaccgcc gaagcgtttc cccgcggtgt atcccaggat tatcgggaag aaccaaaaca
    16741 aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa
    16801 aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc aaaccgatca
    16861 gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta cctttctcat
    16921 cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgttg accgccagga
    16981 agacatcggc cacatggtta cctatgacca cctgaaactg gccaccgctt tccaccacca
    17041 taataatacc gggggtgttt ttcagtacct cagcttgcgc tttgctttca tcctttaact
    17101 taaaacgtaa tcgcgttgcg caatgcatca gactaacaat gttatctgcg cccccgactc
    17161 ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa
    17221 ctccaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt ttcaggtttt
    17281 gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt tctttgcgca
    17341 cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta cgttgatact
    17401 gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg taattttgtt
    17461 ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca agaatacgcc
    17521 aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttct tcctggtaat
    17581 taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc gtgacacctg
    17641 caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca ataaagccca
    17701 cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac gcttcttccc
    17761 ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacggg ttgggcagca
    17821 acacgttttg ctgaaagcgt ttaatcgcaa actggcaatg gtcagttagc gagatataaa
    17881 tactgtcctg taattttccc aagcgctcct gcgctaaaga gataatacga tcacaggttg
    17941 ccatcacctc aagaggaata tgacttaaga gttcgcttaa tcgcccgttc agttcatgac
    18001 tgctcaaggc atactctttt tctattccac ttgagttaat tctttcgcca gcgcgttttt
    18061 gaaagccaat tcctcgcccc atgacgactt tttcccgctg ttgatcatca ataaccacca
    18121 caacattatt gttgagaatt ttggtgattt gcatgttcat agcaaggacc ttttttataa
    18181 acaaaaaaac ccgacttcac cagtattctc tggttatgtc aggttttgcc tgcgaatgca
    18241 gtaacaatcc agtcatttat taatggtttt tataacgaac atccaggttc ggaaattaat
    18301 ttaattgcgt gcagagaaaa tagcaatgcg ctattgataa aaatatgacc atgctcgcag
    18361 ttattaactt tgtgtaattt taggaattta taaagttata tataacaaat cccaataatt
    18421 aagttattgg gatttgtctg gtgaattatt tgtcgctatc ttttcccgcc agcagtttat
    18481 ccagctcatc accgcctaca tgacggaaat cctgcccctt cacgtagtag aagataaact
    18541 cacaaatatt ctggcagcgg tcgccaatac gttcgataga acgcgcgcag aacagcgcag
    18601 taagtacgct cggaatggta cgcgaatctt ccatcatgta ggtcatcagt tgacgaacaa
    18661 taccttcgta ttcctgatcg acttttttat cttcacgata aatacgtacc gcttcgtcaa
    18721 tgtccatccg cgcgaacgcg tccagcacgt cgtgcagcat ctggatggta tgacggccca
    18781 gcgactccag acttaccagc aacggctgat gctgctggga gaatttctcc agcgcagtac
    18841 ggcagatttt gtccgccacg tcgccaatac gctccagctc ggcaatggtt ttactgatca
    18901 ccataaccag tcgcaggtcg ctcgccgtcg gctgacgttt ggcgataatg cgcacgcagg
    18961 cttcatcgat cgccacttcc atcatgttga cgttcttgtc gccttcgatc acgcgcttcg
    19021 ccagatcgct gtcctggtta tgcatcgcgg tgattgcatc agaaagctgc tgctccacca
    19081 tgccgcccat ggtcatcacc tgcgtgcgga tactttccag ttcggcgttg aactggccgg
    19141 aaatatgttt attaagattg agactgtcca taacgcactc ctgaatcaac cgtaacgacc
    19201 ggtgatgtag tcttctgttt gtttcttcgc tggcttggtg aacagatcgt ccgtgttgct
    19261 gaactcaatc aattcgccca ggtacataaa cgccgtgtgg tcggaacaac gcgcagcctg
    19321 ctgcatgttg tgggtgacga tcaccacggt gtaatcctgc ttcagttcgg tgatcagctc
    19381 ttcaatacgc ccggtagaga tagggtcgag cgccgaacac ggttcgtcga gcagcagcac
    19441 ttccgggcga atggcgatac cacgcgcaat acacagacgc tgttgctgac caccagagag
    19501 agagtaaccg ctctggtgca atttatcttt ggtttcgttc cacaatgcgg ctttggtcaa
    19561 tgcccactgc acgcgctcgt ccatgtcggc acgggagagc ttctcaaaca gacgaacgcc
    19621 aaaagcgatg ttgtcgtaga tggacatcgg aaacggcgtc ggtttctgga acaccatgcc
    19681 cactttcgca cgcagcaggg cgatatcctg agagttggtc aggatgttgt cgccatcaag
    19741 cagaatttca ccttccgcac gctgctccgg gtacagttca aacattttgt tgaaggtacg
    19801 cagcagcgtc gatttaccgc agccggacgg cccgataaac gccgttacct ggtttttagc
    19861 gatatccagg ttgatgtttt tcagggcatg gaatttgccg tagtagaagt tcaaattacg
    19921 aacctgaatt ttactcgggg cagtttcaac catactcatt gcaatctttt cctcatcgtg
    19981 cctgatgcac ttcgcgaatc aggcttaccg taggccggat aaggcgttca cgccgcatcc
    20041 ggcaaagtgt taaccgtgtt tattcttcgc aaaaacaacg cgcgccagaa tgttcagcag
    20101 cagtacgcac agggtaataa tcaacacccc ggcccaggcc agttgctgcc attctgcaaa
    20161 cgggctcatc gcaaacttaa agatcgtcac cggcaggttg gcgatcggct gcatcatgtc
    20221 cgtgctccag aactggttgg agagcgcggt aaacagcagc ggcgcggttt caccggcaat
    20281 acgggcaatc gccagcagga taccggtcat gatcccggac accgacgctt tcagcgtaat
    20341 cgcggagatc attttccact tcggtgtacc cagcgcataa gccgcttcac gcaggctgtc
    20401 cggcaccagt ttcagcatgt tctcggtggt gcggataaca atcggcacct gcaacagcgc
    20461 cagcgcaatt acacccgccc agccagagaa gtgctccatc tgcgccacca cgatggtgta
    20521 aacaaacaga ccaaccacaa tcgacggcgc ggagagcaga atgtcgttaa tgaagcgaat
    20581 cacttctgcc agccaggatt tacgaccata ttccgccaga taaatccccg ccataatgcc
    20641 cagcggcgta ccgaataccg tggcccacaa aattaacagc ccgctacccg ccagggcgtt
    20701 cgccagacca ccaccttccg tattgggcgg cggcgtcatt tcagtgaaca gcgccagcga
    20761 cataccgtcg ataccgcgag tgatggtgga cattaaaatc cagatcagcc agaacaggcc
    20821 gaaggccatc gtcgccatcg agagcgtcag cgcaatacgg tttttgaggc ggcgacgcgc
    20881 ctgcattttg cggcgagatt cagccagcgc cgcagtggtt tgcatttcaa ccatagccat
    20941 tagcgtgccc cctcattctt agccaggcgc ataatcataa acttcgatgc ggcgaggacg
    21001 atgaaggtaa tcacaaacag gatcaggccc agttccatca gtgcggcaac gtgcagaccg
    21061 gattccgctt ccgcaaattc gttcgccagc gcagaggtga tactgttgcc cggcatatac
    21121 agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa aggtcaccgc catggtttca
    21181 ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa caccattttt ggtgaacgga
    21241 agaacgatac gccagataac ttcccaggtg gtgcagccaa taccgtaggc cgactctttc
    21301 atcatcaccg gggtttgttc gaacacatca cgcattaccg ccgcaatgta cggaataatc
    21361 atgatggcga ggatcacgcc tgccgcgagg ataccgatac caaatgcggg gccagagaac
    21421 agcgcgccaa caatcgggat attcgacatg atattgccga ccggctcctg aaagtaaacg
    21481 gcgaacagcg gcgcaaagat aaacaggccc cacatgccgt aaacgatact tggaatggct
    21541 gccagcagct caatggcgat acccagcggg cgtttcagcc agccaggcgc aagctcagtc
    21601 aggaacaggg cgataccgaa actcaccggg acggcgatca gcagcgcgat aaacgaagtc
    21661 accaacgtac cgtagatcgg caccagcgcc ccgtagatat cgttcggtgc atcccactct
    21721 ttggtccata ggaaagccag accaaatttc tgaatgctcg gccaggagga gatgatcaga
    21781 gagacaataa tgccacccaa catcaatagc acaatcagcg ccgccagttt taccagcacg
    21841 ctgaaaatta tgtcgccctt tttacccggt gggttaaaag caggcttggt tgcagccata
    21901 agttactctt cagttaaacg cgtttacaaa gtcattttgt aatgccggat gcggcgtaaa
    21961 acaccgtacc cggcctggag ttttattagt acagcggctt accgctactg tctttaatat
    22021 tggtcttcca cgcagcgcga acctgttcaa ctacgctatc cggcaggctg gcgtaatcca
    22081 ggtcgttcgc ctgtttagcc ccggttttgt aagcccagtc gaagaacttc agcacttctg
    22141 tgccttgttc tggtttcttc tgatctttgt ggatcagaat gaacgtggta gaggtaatag
    22201 gccatgcatc ttcgcctttc tggttggtca gatcctgagc gaaggttttg ctccagtctg
    22261 caccttttgc tgcattagcg aagttttctt cggtcggact aaccggttta ccatcagcgg
    22321 agatcagttt ggtgtacgcc aggttgttct gcttcgcgta agcatattca acataaccaa
    22381 ttgcacccgg cagacgctga acgaacgcgg cgataccgtc gttaccttta ccgcccagac
    22441 cgatcggcca ttttacggta gagccagtac caacgttgtt tttccactct tcgttcactt
    22501 tcgccaggta gctggtgaag acgaaggaag tcccggagcc atctgcgcgg cgtactacag
    22561 caatgttttg tgaaggcagt ttcagacccg gattcagttt ggcgatggct tcatcatccc
    22621 acttcttgat tttgcccagg tagatgtcgc cgagggtttt accatccagc accagttcgc
    22681 cagacttcag ccctggaatg ttaaccgcca gcaccacgcc gccaatcacg gtcgggaact
    22741 ggaacagacc ttcctgagcc agtttttcgt cagacagcgg cgcgtcagag gcaccaaaat
    22801 caacggtatt agcgataatc tgttttacgc caccggaaga accgataccc tggtagttaa
    22861 ctttattacc ggtttctttc tggtaagtgt cagcccattt ggcatacacc ggcgcaggga
    22921 aggttgcacc tgcacctgtc aggcttgctt ctgcaaacac agagaaagcg ctcatcgata
    22981 aggtcgcggc gacaacagtt gcgacggtgg tacgcataac tttcataatg tctcctggga
    23041 ggattcataa agcattgttt gttggctacg agaagcaaaa taggacaaac aggtgacagt
    23101 tatatgtaag gaatatgaca tttttatgac agaaaggaat taccaaataa cttactgatt
    23161 ttaaatgtga agatgaaaat cccacatgtc atctttttgt catgatgcat ttaaaagtga
    23221 taaagaaatg ggtgagatga caacgatgtg atgaacaatc gttgtcagac ctattagcgc
    23281 acaatctcaa cagttatcgt cgctgttgca gtaaattgcc ccggggctgg gcgagctcca
    23341 gtaaggctcg caggagcagc tgaaaaagtt acactacctg atttttcacc atacactgtt
    23401 gatttaccca tgtctgttgg caactcacca ccgttcactt tgatttcctt attatttttg
    23461 tcgtaaattt ttatccccac gttagagttt gaagttacga ccattgaatt gtccgtattg
    23521 ggttcggtag cataaaaaga cgtactgata tcatcggtat tcagtatccc caggttttca
    23581 cactcaatag tgaccgtttt agtaatttta tgtgttgtta ccgcacttcc aacaacagat
    23641 gaaaaatctg cagctggtat ttctttaaaa tcaaaataaa ctggctgtaa attatttatt
    23701 tcgcaacttt gcggagctgt aatatcgccc cgaatataaa catcagccat cggctgtgag
    23761 taatccaccg gggtgtcacg gctaattgtt ccatacaatg tcactatctt tgtttcagga
    23821 ataatcaatt ttcctgcaaa tgtcttcttg atataaaaat atactatcgc tttagcccca
    23881 ctagatatac ttaaaggttc aatgcggttg caattgtact ttgtattacc agacgggttg
    23941 ttagcatgat attcaaaagg tgcattcaca tacccaaccc caagaatatc tatagcaatc
    24001 ccaacatcaa ggttcggatt aagatcataa taattaatat tagagtgaac cccagcataa
    24061 gttaatgcct ggtttatttt tgacgtataa tagatatagg ggaattctcc tacgttacca
    24121 gtatcacaat gacagcgtcc aggatatgag gatccattag ttacagtggt gttaaatgtc
    24181 gttccaacct tattttcgtt cgcggtaata agatatcccg agaaatcaag cgtactgtga
    24241 aatacgccat tatcaggggt acagatacct ttaggagctg caagtgctac actacttaac
    24301 gagagcataa acacagcaaa gcaccactgt ttgatatact tgttcataaa aatcctttta
    24361 atgacatgtc acaccctgtt caattaatcc gctctcactt tctggtgtcg aaagtgaata
    24421 attgaagatg cattttgttg aggcgttttc tccccaggat aacgtaacct gtcctttttc
    24481 tggtagacca gaaagataaa gttcgccacg gtcaccgaca attcctgaat ctgccaaact
    24541 tgcatcttgt atcgcagcaa tggctccaaa aggtaccggt ttaccatttg catttaacac
    24601 tctgaagaga acgcgataac ccacatgagt cacaaactcc gctttaacca ctgcaccacg
    24661 cgtgggaacc atcgtaacga cggtattatc aagttctata ttcgacttcg agaaatagtt
    24721 atcgctgatt gctacccgat tttcatcata agggtttagc tgtgtcttga ccgtatagcc
    24781 gcgccagtcc gtcgcagcac cgcgcatatt atctatttct aacccggcgg ccccaggtgc
    24841 tttaacaaga gcaatagttt cacctaactc ctgacttaac gttacccctt cgctgtgtgc
    24901 cagaattccc cctctgatac cataattcag cctctgggaa ttttggctgt agttatagct
    24961 gccgttcacc gtgccataag agccatcata ggtcagggac atattaccgc caacctcatc
    25021 atcatgactg ttataggact gcattaaatt ccatgaaagg ttatttcttt ccagcgccgt
    25081 accgctgagt ccgagttgat gtgagctacc ggaattatcg ttttgcgaaa cagaataggt
    25141 ggcgtaacta ttaccccacc agcccgataa aggaacagat aaagagagca tgtagatctg
    25201 ctcactggtg ttatcactgg catggttgtt ccccgtcccc tgtctggcag tattggaata
    25261 actccaggag acgttccacg agagacgctt ccagctactg ctgtagccaa attgcattaa
    25321 tcgttcgaca tcatcggtat gccagtattg ttgccggctt aatgtcaggt ataaattgcc
    25381 ataatctctc cccaacgact gcgttagcgt tccctccacg cgactacgtc ggttacctgt
    25441 ttcccaaaag tcttcagggc tgttgcgccg cgatgcccac tcattgaggg tatagtagcc
    25501 ctcggtggag taacgatagc caaccaggcg caagctagtg tctgtgtcgt caaatcgttt
    25561 tgaatagaga aagcgatatg actgacccgt ttctgtatca tcatggctaa aattcgcacg
    25621 agcatgtgtc acatcgaaag aaattgcgcc caaagcgcca agatctttac cgacacccag
    25681 agctgcagaa ccataatttt cagcagcctg catacctcca tataaagtga tcccgtggga
    25741 aaaaccatga agtatttgcg cctgaaggac gtccggtgta aatccgtctt catcgcgcaa
    25801 ttcacccacg ctgacatcaa catctgtctg accttcacgt ttaagaatcg ccaatgaagc
    25861 ataaggttgg gtgaatcgtt gttcgctacc gtcttcttct ttgatggtta cgtccaaatc
    25921 gccacctgta ctggcggtgt tgagatcggt gatctcaaaa gcaccttgtg gaacggcaga
    25981 ctgataaatc acgtagccat tctgccgaat gatgacggtg gcgttggact ttgcaatccc
    26041 acgaaccaca ggagcaaagc cattctggct ggcgggtagc atatcgttat cagtatacaa
    26101 acgcgcgccg cgaatttgcg tactgtcgaa aatatcgctc gccgtccacg tatcaccaat
    26161 cataatctgg cttcttaacg cggcaatatc gcgctgtatc caggtactaa tggagttgta
    26221 ttccgcacgg ccatcgctgg tgtttaaagt actgtaatta cgtagccgcc acggtccaat
    26281 attcagtcca ttgcgcagat tgagataact ggtatctgtc tcttgtgaac cataatctga
    26341 acgtagtgta tgtgaaccag aaaaatcgta gttgatggtg agcgcgttaa tcccctcatc
    26401 ccaacgcgtg gggctgatgt aatcataagc catcgcgtcc atgtagattt ggggaatgga
    26461 tatattcagc tgcaatttat cactgtcaaa ctgccaggat gagccttcaa tacgtggagc
    26521 aggatcgagg caaaccgtct catccacatc attgaacgat ttgagcgcat caacttttat
    26581 gccaaaggag gacagtgttt ttttaatggc aataaagcat gcgtgcagat cagcatctgg
    26641 attatcctga acaaatgtca catcctgacg ttcaaacatt ttaccgttga taaaaatatc
    26701 aacgctataa acacctgggg gcatcaggtt tgcccgatta aactggcgaa tatcgactcc
    26761 ggattgatgg tcgatttcca gaagcgccgg attgaataca ctgacctcgg ccatcaggtt
    26821 ttgcgagaaa atcagacacg tccctgccgt gaggccaacc actattctgg tcgtcatcat
    26881 aatacgccta tccttatccc ttgcttacag cgtcattttt atcgggtcac tgactgcgcc
    26941 gtaatcatta ataaatcgcc aggtcatttg gcctcccgca gaattagcag gtaaaggtaa
    27001 cgtgagcgtt tcaaaaggac ggatcatatt gaggggcaca tcatggccat caacgatgac
    27061 atccatcaca ctgatatgaa ttgggttggg attctctgct atcagatttc gcccttcccg
    27121 gcgccatttc actttcggcg cagcatcttc gggaataagg cggatatttg ctggtcgcca
    27181 gaacagcttg atacgcgttt ttactgctat ttccagcgag ttcgtcgctt tgttgctcgc
    27241 tgatggcgga atatttttaa tattcagcca gaagactgac tcgcgatcat caggcaatga
    27301 cacacctttg gtgtgaataa tgcgtaatat tttttcttgc gaaggttcta atcgtgatac
    27361 tggcggaata acggtaaacg gcgctttgct tttatcctct ttattaaatg gatctaccca
    27421 agcctggatg agataaggta ttttttcttc tttgctaaat acagaaatag atttatcagc
    27481 attattactt aagtaaacca cccgcgtacc accaatgaca acgcctgcat aagttaatgc
    27541 tggttggcct aaaaaaacgc tgagtataat cgcggtaaat aacttacatg aaactgttgt
    27601 tttaaagagc ctgattaaac aactttttct ggacataacg actccctggc caaagccaac
    27661 tgaataaaaa ggcccttgat aaattatttc ttatattcga cagtaaattg tgaatcagcg
    27721 ttcgccgtac cagccgttac ggtcgcattg gttgccacat agcgtgcaat aaacttcatt
    27781 tcagccttgc cttcagcatc gatagcggta taagcagagt cgttataaag tttaacctgc
    27841 gaattatcgg actggttaaa aatacgaata ccaacgccgg ttgccgcaat cgcttgtcct
    27901 gtaccggtaa aatcaccagg gttgcctgct gtaagcggcg tacccacttt caggtcgccg
    27961 tcgccgccag gcgcttctgt accatcaaaa cgaacggctg cttgagtgta cgttgccggg
    28021 caattttcaa gcttaatact gaacttctgt agcgacgcca gactacctac acctgaaaat
    28081 gcatttttac tgactttacc cagtgggaca atttgatcgg ctgtatcgga ggtaacttca
    28141 caagtagtgt cgacaatttc accgacgaag tgaacaacaa catcttctgc cagtgcatgc
    28201 ccggccagca ttagagatgc cagagtgaat gcaccgccta taatattacg cttcataaaa
    28261 actccattga tataatatat cctttaccat gaatcatcac ttatattaat aagtgtattt
    28321 ttgattcaaa ggaatagtac accaaagaga cggctttatc taaaaataat attaattacg
    28381 caaaaataaa atataaattt attgt
//
LOCUS       DAZFVO020000056        27928 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFVO020000056 DAZFVO020000000
VERSION     DAZFVO020000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 27928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.056"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            40..363
                     /locus_tag="ACHKNB_RS11025"
                     /old_locus_tag="pgaptmp_002205"
     CDS             40..363
                     /locus_tag="ACHKNB_RS11025"
                     /old_locus_tag="pgaptmp_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HGU4195708.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(466..1716)
                     /gene="icd"
                     /locus_tag="ACHKNB_RS11030"
                     /old_locus_tag="pgaptmp_002206"
     CDS             complement(466..1716)
                     /gene="icd"
                     /locus_tag="ACHKNB_RS11030"
                     /old_locus_tag="pgaptmp_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HGU4195709.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1888..2541
                     /gene="rluE"
                     /locus_tag="ACHKNB_RS11035"
                     /old_locus_tag="pgaptmp_002207"
     CDS             1888..2541
                     /gene="rluE"
                     /locus_tag="ACHKNB_RS11035"
                     /old_locus_tag="pgaptmp_002207"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HGU4195710.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2551..3012
                     /gene="nudJ"
                     /locus_tag="ACHKNB_RS11040"
                     /old_locus_tag="pgaptmp_002208"
     CDS             2551..3012
                     /gene="nudJ"
                     /locus_tag="ACHKNB_RS11040"
                     /old_locus_tag="pgaptmp_002208"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HGU4195711.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3066..4172
                     /gene="mnmA"
                     /locus_tag="ACHKNB_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /gene_synonym="trmU"
     CDS             3066..4172
                     /gene="mnmA"
                     /locus_tag="ACHKNB_RS11045"
                     /old_locus_tag="pgaptmp_002209"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGU4195712.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4208..4849
                     /gene="hflD"
                     /locus_tag="ACHKNB_RS11050"
                     /old_locus_tag="pgaptmp_002210"
     CDS             4208..4849
                     /gene="hflD"
                     /locus_tag="ACHKNB_RS11050"
                     /old_locus_tag="pgaptmp_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HGU4195713.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4853..6223
                     /gene="purB"
                     /locus_tag="ACHKNB_RS11055"
                     /old_locus_tag="pgaptmp_002211"
     CDS             4853..6223
                     /gene="purB"
                     /locus_tag="ACHKNB_RS11055"
                     /old_locus_tag="pgaptmp_002211"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGU4195714.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6391..7062
                     /gene="phoP"
                     /locus_tag="ACHKNB_RS11060"
                     /old_locus_tag="pgaptmp_002212"
     CDS             6391..7062
                     /gene="phoP"
                     /locus_tag="ACHKNB_RS11060"
                     /old_locus_tag="pgaptmp_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HGU4195715.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7062..8522
                     /gene="phoQ"
                     /locus_tag="ACHKNB_RS11065"
                     /old_locus_tag="pgaptmp_002213"
     CDS             7062..8522
                     /gene="phoQ"
                     /locus_tag="ACHKNB_RS11065"
                     /old_locus_tag="pgaptmp_002213"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HGU4195716.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8598..9719
                     /gene="roxA"
                     /locus_tag="ACHKNB_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /gene_synonym="ycfD"
     CDS             8598..9719
                     /gene="roxA"
                     /locus_tag="ACHKNB_RS11070"
                     /old_locus_tag="pgaptmp_002214"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HGU4195717.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9865..11094)
                     /gene="pepT"
                     /locus_tag="ACHKNB_RS11075"
                     /old_locus_tag="pgaptmp_002215"
     CDS             complement(9865..11094)
                     /gene="pepT"
                     /locus_tag="ACHKNB_RS11075"
                     /old_locus_tag="pgaptmp_002215"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGU4195718.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11344..12480
                     /gene="potA"
                     /locus_tag="ACHKNB_RS11080"
                     /old_locus_tag="pgaptmp_002216"
     CDS             11344..12480
                     /gene="potA"
                     /locus_tag="ACHKNB_RS11080"
                     /old_locus_tag="pgaptmp_002216"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HGU4195719.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12464..13321
                     /gene="potB"
                     /locus_tag="ACHKNB_RS11085"
                     /old_locus_tag="pgaptmp_002217"
     CDS             12464..13321
                     /gene="potB"
                     /locus_tag="ACHKNB_RS11085"
                     /old_locus_tag="pgaptmp_002217"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HGU4195720.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13318..14112
                     /gene="potC"
                     /locus_tag="ACHKNB_RS11090"
                     /old_locus_tag="pgaptmp_002218"
     CDS             13318..14112
                     /gene="potC"
                     /locus_tag="ACHKNB_RS11090"
                     /old_locus_tag="pgaptmp_002218"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HGU4195721.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14109..15155
                     /gene="potD"
                     /locus_tag="ACHKNB_RS11095"
                     /old_locus_tag="pgaptmp_002219"
     CDS             14109..15155
                     /gene="potD"
                     /locus_tag="ACHKNB_RS11095"
                     /old_locus_tag="pgaptmp_002219"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HGU4195722.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            15213..15674
                     /locus_tag="ACHKNB_RS11100"
                     /old_locus_tag="pgaptmp_002220"
     CDS             15213..15674
                     /locus_tag="ACHKNB_RS11100"
                     /old_locus_tag="pgaptmp_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HGU4195723.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15671..16459
                     /locus_tag="ACHKNB_RS11105"
                     /old_locus_tag="pgaptmp_002221"
     CDS             15671..16459
                     /locus_tag="ACHKNB_RS11105"
                     /old_locus_tag="pgaptmp_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HGU4195724.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16598..17419)
                     /gene="cobB"
                     /locus_tag="ACHKNB_RS11110"
                     /old_locus_tag="pgaptmp_002222"
     CDS             complement(16598..17419)
                     /gene="cobB"
                     /locus_tag="ACHKNB_RS11110"
                     /old_locus_tag="pgaptmp_002222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HGU4195725.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17435..18346)
                     /gene="nagK"
                     /locus_tag="ACHKNB_RS11115"
                     /old_locus_tag="pgaptmp_002223"
     CDS             complement(17435..18346)
                     /gene="nagK"
                     /locus_tag="ACHKNB_RS11115"
                     /old_locus_tag="pgaptmp_002223"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HGU4195726.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18375..19619)
                     /gene="lolE"
                     /locus_tag="ACHKNB_RS11120"
                     /old_locus_tag="pgaptmp_002224"
     CDS             complement(18375..19619)
                     /gene="lolE"
                     /locus_tag="ACHKNB_RS11120"
                     /old_locus_tag="pgaptmp_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HGU4195727.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19619..20320)
                     /gene="lolD"
                     /locus_tag="ACHKNB_RS11125"
                     /old_locus_tag="pgaptmp_002225"
     CDS             complement(19619..20320)
                     /gene="lolD"
                     /locus_tag="ACHKNB_RS11125"
                     /old_locus_tag="pgaptmp_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HGU4195728.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(20313..21512)
                     /gene="lolC"
                     /locus_tag="ACHKNB_RS11130"
                     /old_locus_tag="pgaptmp_002226"
     CDS             complement(20313..21512)
                     /gene="lolC"
                     /locus_tag="ACHKNB_RS11130"
                     /old_locus_tag="pgaptmp_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HGU4195729.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21774..22847
                     /locus_tag="ACHKNB_RS11135"
                     /old_locus_tag="pgaptmp_002227"
     CDS             21774..22847
                     /locus_tag="ACHKNB_RS11135"
                     /old_locus_tag="pgaptmp_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGU4195730.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLTLWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            22975..26421
                     /gene="mfd"
                     /locus_tag="ACHKNB_RS11140"
                     /old_locus_tag="pgaptmp_002228"
     CDS             22975..26421
                     /gene="mfd"
                     /locus_tag="ACHKNB_RS11140"
                     /old_locus_tag="pgaptmp_002228"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGU4195731.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDKLFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26566..27528
                     /gene="ldtC"
                     /locus_tag="ACHKNB_RS11145"
                     /old_locus_tag="pgaptmp_002229"
     CDS             26566..27528
                     /gene="ldtC"
                     /locus_tag="ACHKNB_RS11145"
                     /old_locus_tag="pgaptmp_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HGU4195732.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27611..27868)
                     /gene="bhsA"
                     /locus_tag="ACHKNB_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /gene_synonym="ycfR"
     CDS             complement(27611..27868)
                     /gene="bhsA"
                     /locus_tag="ACHKNB_RS11150"
                     /old_locus_tag="pgaptmp_002230"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HGU4195733.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6864 a         7208 c         7025 g         6831 t
ORIGIN      
        1 aatgaactat attaaatacc gtgggataag acataacaaa tgaagtggat agtaattgac
       61 acgataattc aaccttcatg cggcatatct ttttcagcca tatggggtaa tatgaaaatg
      121 atcatctggt atcaatctac tatatttctc cctcctggca gtatatttac accggttaaa
      181 tctggaatta tccttaaaga taaagaatat cctattacta tttataacat cgcaccattc
      241 aacaaggatt tatggagtct actcaaaagc agtcaagagt gtcctccagg agaaagcaaa
      301 ataacaaata aatgtttgca taatagttgc attataaaaa tatgcccata tgggctcaag
      361 taaagggttt tatataaata tgctcgatat gctattaata atacctacat aaataaaaca
      421 acgggcgtgt tatacgcccg ttgcaatatt aaacacatgt agtgattaca tgttcttgat
      481 gatcgcgtca ccaaattctg aacatttcag cagtttagcg ccttccatca gacgctcgaa
      541 gtcataggtt acggtcttcg cattgattgc gccttccata cctttaacaa tcaggtctgc
      601 ggcttcagtc cagcccatat ggcgtaacat catctcagcg gagagaataa tagagccagg
      661 gtttactttg tcctgaccgg cgtatttcgg cgcagtaccg tgggtggctt caaacagggc
      721 gcattcgtca ccgatgtttg ctccaggggc gataccgata ccgccaacct gcgctgccag
      781 agcgtcagaa atgtagtcac cgttcaggtt catacaggcg ataacatcat attcagccgg
      841 acgcagcagg atctgttgca ggaatgcatc agcaatcacg tctttaatga cgatctcttt
      901 gccggtgttc gggtttttaa ctttcagcca cgggccgccg tcgatcagtt caccgccaaa
      961 ctcttcacgc gccagctggt agccccagtc tttaaacgcg ccttcggtga acttcatgat
     1021 gttgcctttg tgcaccagag tcacagagtc acgatcgtta gcaattgcgt attcgatcgc
     1081 tgcacgaacc agacgtttgg tgccttcttc cgaacacggc ttaataccga taccacaatg
     1141 ttccgggaag cgaattttct tcacccccat ctcttcacgc aggaatttaa tcactttctc
     1201 ggcgtcggca gagtctgctt tccattcgat acccgcataa atgtcttccg agttttcacg
     1261 gaagataacc atatcggtca gttcagggtg tttaaccggg cttggagtgc cctgatagta
     1321 acgtaccgga cgcaggcaga tgtagagatc cagttcctgg cgcagggcaa cgttcagaga
     1381 gcgaataccg ccaccaacag gagtggtcag cggaccttta atggcaacgc gatattcacg
     1441 aatcagatca agggtttcag caggcagcca gacatcctga ccataaacct gtgtggattt
     1501 ttcaccggtg taaatttcca tccaggagat tttacgctcg cctttatagg ctttctcgac
     1561 tgcagcgtcg accactttca gcatggctgg ggttacatct acaccgattc catcaccttc
     1621 aatgtaaggg ataatcggat tttcaggaac gttgagtttg ccgttttgca gggtgatctt
     1681 cttgccttgt gccggaacaa ctactttact ttccattcac ctctccttcg agcgctactg
     1741 gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg gatgctttag
     1801 agcaattttt tgttaatgat ttgtaattgg cttgtcaata ctaccctatt gtttgccgtc
     1861 atgaaagatg cgcgttatta ggctataatg cggcaattca taatctctga aaataccatg
     1921 caaaaaactt cttttagaaa tcaccaggtt aagcgattca gctcgcaacg ttctaccagg
     1981 cgtaaacctg aaaaccagcc cacgcgtgtg atcctgttca ataaacccta cgatgttctt
     2041 ccgcagttca ccgatgaagc cggacgcaaa acattaaaag aattcatccc ggttcagggc
     2101 gtttatgcag caggtcgtct tgaccgcgat agcgaagggt tactggtgct gaccaataac
     2161 ggcgcgttgc aggcgcgttt aacccagccg ggcaaacgca ccgggaaaat ctattatgtg
     2221 caggtggaag gtattcccac acaagacgca cttgaagcct tgcgcaatgg cgtaacctta
     2281 aatgatggcc ctaccctgcc cgcaggcgcg gagctggttg acgaacccgc gtggttatgg
     2341 ccgcggaatc caccaattcg tgaacgcaaa agtattccca ccagctggct gaagatcacc
     2401 ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg tttccccacg
     2461 ctgcgactga ttcgctatgc gatgggagat tactctttgg ataatcttgc caatggcgaa
     2521 tggcgagaag tgacagatta aggatgcaaa atgtttaaac cgcacgttac cgttgcttgc
     2581 gtggtgcacg cagaaggcaa atttttagtc gttgaagaga cgattaatgg taaagcgtta
     2641 tggaaccaac ctgccgggca tctggaagcc gatgaaacct tagtggaagc cgccgcccgt
     2701 gaactgtggg aagaaaccgg catcagcgcg cagccgcaac actttattcg tatgcatcag
     2761 tggattgcgc cagataaaac gccgtttttg cgtttcctct ttgccattga gcttgagcaa
     2821 atatgcccga cccagcctca tgacagcgat atcgactgct gtcgttgggt cagcgccgaa
     2881 gaaattttac aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat ccgttgttat
     2941 caaagcgggc aacgctatcc gctggagatg attggcgatt ttaactggcc ttttacaaag
     3001 ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt gaagttcaat gtcgtgagtg
     3061 atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt gtcgattcct
     3121 ccgtttctgc ctggctgttg caacaacagg gatatcaggt tgaaggcctg tttatgaaga
     3181 actgggaaga agacgacggt gaggaatatt gcacagcggc agcggatctg gctgatgccc
     3241 aggctgtctg cgacaagctc ggcattgaac tgcacaccgt taactttgct gccgagtact
     3301 gggacaacgt cttcgaactg ttccttgccg aatataaagc cggtcgcacg ccgaatccgg
     3361 atattctgtg caacaaagag atcaaattta aagccttcct cgaatttgcc gccgaagatt
     3421 taggtgccga ttatatcgct accggtcatt acgtacgtcg ggccgatgtc gatggcaaga
     3481 gccgcctgct gcgtggtctg gacagcaata aagaccagag ctacttcctt tatacgctca
     3541 gccatgagca gattgcgcaa agcctgttcc cggtcggcga actggaaaaa ccgcaggtgc
     3601 gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa aaaagactct accggcattt
     3661 gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg ttatctcccg gcgcaaccgg
     3721 gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca ccaggggctg atgtatcaca
     3781 ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa agaaggtact gaagaaccgt
     3841 ggtatgtggt ggacaaagac gtcgaaaaca acattctggt tgtcgctcag ggccatgaac
     3901 acccgcggct gatgtctgtc gggttgattg cccagcagtt gcactgggtc gatcgcgaac
     3961 cattcaccgg cactatgcgt tgcacggtaa aaacccgcta tcgccagacc gatatccctt
     4021 gcaccgtcaa ggcgctggac gatgatcgca ttgaagtgat tttcgatgaa ccggttgccg
     4081 ccgtgacgcc gggccagtct gccgtcttct ataacggtga agtgtgcctc ggtggcggta
     4141 ttattgagca gcgtctgccg ctgccggtct gattattatc tttacttaaa acaaggaagc
     4201 agtgaacgtg gcaaagaatt actatgacat caccctcgcc ctggccggta tttgtcagtc
     4261 ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt gatgccgatg cgctacacgt
     4321 ctcactcaac agtattattg atatgaaccc cagctcgacg ctggcggttt ttggcggtag
     4381 cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc gtgctcaatg ccagcagtcg
     4441 ccagggctta aacgctgagt taacccgcta cacactcagc ttgatggtgc ttgagcgcaa
     4501 actctcctca gcgaaaggcg cgctcgacac tctgggcaac cggatcaacg gcctgcaacg
     4561 ccagctcgaa cacttcgatt tacagtccga aacgctgatg agcgcgatgg ctgctatcta
     4621 tgttgatgtg atcagcccgc ttggcccgcg cattcaggtc accggttccc ctgctgtact
     4681 gcaaagccca caagtgcagg cgaaagttcg cgcaaccctg ctggcaggca ttcgcgccgc
     4741 cgtgctctgg caccaggtcg gcggcggacg tctgcaactg atgttttctc gtaatcgcct
     4801 gaccactcag gcaaaacaaa ttcttgctca tttaaccccg gagttgtgat ctatggaatt
     4861 atcctcactg accgccgttt cccctgtcga tggacgctac ggcgataaag tcagcgcgct
     4921 gcgcgggatt ttcagcgaat atggtttgct gaaattccgt gtacaagttg aagtacgttg
     4981 gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt cctgcttttg ctgccgacgc
     5041 aatcggttac cttgatgcaa ttgtcgccaa tttcagcgaa gaagatgccg cacgcatcaa
     5101 aaccatcgag cgtaccacta accacgacgt taaagcggtt gagtatttcc tgaaagaaaa
     5161 agtggcggag atcccggaac tgcacgcggt ttctgaattc atccactttg cctgtacttc
     5221 ggaagatatc aataacctct cccacgcatt aatgctgaaa accgcgcgtg atgaagtgat
     5281 cctgccgtac tggcgtcaac tgattgatgg cattaaagat ctcgccgttc agtaccgcga
     5341 tatcccgctg ctgtctcgta cccacggtca gccagccacg ccgtcaacca tcggtaaaga
     5401 gatggcaaac gtcgcctacc gtatggagcg ccagtaccgc cagcttaacc aggtggagat
     5461 cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc cacatcgccg cttacccgga
     5521 agttgactgg catcagttca gcgaagagtt cgtcacctcg ctgggtattc aatggaaccc
     5581 gtacactacc cagattgaac cgcacgacta cattgccgaa ctgtttgatt gcgttgcgcg
     5641 cttcaacacc attctgatcg actttgaccg tgacgtctgg ggttatatcg cccttaacca
     5701 cttcaaacag aaaaccattg ctggtgagat tggttcttcc accatgccgc ataaagttaa
     5761 cccgatcgac ttcgaaaact ccgaagggaa tctgggcctt tccaacgcgg tattgcagca
     5821 tctggcaagc aaactgccgg tttcccgctg gcagcgtgac ctgaccgact ccaccgtgct
     5881 gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg tatcaatcca ccctgaaagg
     5941 cgtgagcaaa ctggaagtga accgtgacca tctgctggat gagctggatc acaactggga
     6001 agtgctggca gaaccaattc agacagttat gcgtcgctat ggcatcgaaa aaccgtacga
     6061 gaagctgaaa gagctgactc gtggtaagcg cgttgacgcc gaaggcatga agcagtttat
     6121 cgacggtctg gcgttgccag aagaagagaa agcccgcctg aaagcgatga cgccggcaaa
     6181 ctacattggt cgcgccatca ccatggttga tgagctgaaa taaactcgta tcagtgccgg
     6241 atggcgatgc tgtccggcct gcttattaag attatccgct ttttattttt tcactttacc
     6301 tcccctcccc gctggtttat ttaatgttta cccccataac cacataatcg cgttacacta
     6361 ttttaataat taagacaggg agaaataaaa atgcgcgtac tggttgttga agacaatgcg
     6421 ttgttacgtc accaccttaa agttcagatt caggatgctg gtcatcaggt cgatgatgca
     6481 gaagatgcca aagaagccga ttattatctc aatgaacatt taccggatat tgcgattgtc
     6541 gatctcggat tgccagacga ggacggtctg tcactgattc gccgctggcg tagcaatgat
     6601 gtttcactgc cgattctggt attaaccgcc cgtgaaagct ggcaggacaa agtcgaagta
     6661 ttaagtgctg gtgctgatga ttacgtgact aaaccatttc atattgaaga ggtgatggcg
     6721 cgaatgcagg cattaatgcg gcgtaatagc ggtctggctt cacaggtcat ttcgctacca
     6781 ccgtttcagg ttgatctctc tcgccgtgaa ttatctatta atgacgaagt gatcaaactg
     6841 accgcgttcg aatacaccat tatggaaacg ttgatacgca ataatggcaa agtggtgagc
     6901 aaagattcgt taatgctcca actctatccg gatgcggagc tgcgggaaag ccataccatt
     6961 gatgtactga tgggacgtct gcgcaaaaaa attcaggcac aatatccgca agaagtcatt
     7021 accaccgttc gcggccaggg ctacctgttc gaattgcgct gatgaaaaaa ttactgcgtc
     7081 tttttttccc gctctcgctg cgggtacgtt ttctgttggc aacggcagcg gtagtactgg
     7141 tgctttcgct tgcctacgga atggtcgcgc tgatcggtta tagcgtcagt ttcgataaaa
     7201 ctacgtttcg gctgttacgt ggcgagagca atctgttcta tacccttgcg aagtgggaaa
     7261 acaataagtt gcatgtcgag ttacccgaaa atatcgacaa gcaaagcccc accatgacgc
     7321 taatttatga tgagaacggg cagcttttat gggcgcaacg tgacgtgccc tggctgatga
     7381 agatgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
     7441 acgataccag cctcttgctg agtggagatc attcgataca gcaacagttg caggaagtgc
     7501 gggaagatga tgacgacgcg gagatgaccc actcggtggc ggtaaacgtc tacccggcaa
     7561 catcgcggat gccaaaatta accattgtgg tggtggatac cattccggtg gagctaaaaa
     7621 gttcctatat ggtctggagc tggtttatct atgtgctctc agccaatctg ctgttagtaa
     7681 tcccgctgct gtgggtcgcc gcctggtgga gtttacgccc catcgaagcc ctggcaaaag
     7741 aagtccgcga actggaagaa cataaccgcg aattgctcaa tccagccaca acgcgagaac
     7801 tgaccagtct ggtacgaaac ctgaaccgat tgttaaaaag tgaacgcgaa cgttacgaca
     7861 aataccgtac gacgctcacc gacctgaccc atagtctgaa aacgccactg gcggtgctgc
     7921 aaagtacgct gcgttctctg cgtagtgaaa agatgagcgt cagtgatgct gagccggtaa
     7981 tgctggagca aatcagccgc atttcacagc aaattggcta ctacctgcat cgtgccagta
     8041 tgcgcggcgg gacattgctc agccgcgagc tgcatccggt cgccccactg ctggacaatc
     8101 tcacctcagc gctgaacaaa gtgtatcaac gcaaaggggt caatatctct ctcgatattt
     8161 cgccagagat cagctttgtc ggtgagcaga acgattttgt cgaggtgatg ggcaacgtgc
     8221 tggataatgc ctgtaaatat tgcctcgagt ttgtcgaaat ttctgcaagg caaaccgacg
     8281 agcatctcta tattgtggtc gaggatgatg gccccggtat tccattaagc aagcgagagg
     8341 tcattttcga ccgtggtcaa cgggttgata ctttacgccc tgggcaaggt gtagggctgg
     8401 cggtagcccg cgaaatcacc gagcaatatg agggtaaaat cgtcgccgga gagagcatgc
     8461 tgggcggtgc gcggatggag gtgatttttg gtcgccagca ttctgcgccg aaagatgaat
     8521 aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt gcagatacca
     8581 gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat tttcttgaac
     8641 gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt attgacccga
     8701 tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt cgactggtca
     8761 gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac gatcatctcg
     8821 gtgaaaccaa ctggtcatta ctggtacagg cagtaaacca ctggcatgag ccgaccgccg
     8881 cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg atgatctctt
     8941 tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg tttatcattc
     9001 agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa cagcactgcc
     9061 cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa gagctggagc
     9121 ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg ctggaaaatg
     9181 cgatgaacta ttccgtgggc tttcgcgcgc caaatacgcg ggaactgatt agtggatttg
     9241 ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatcct gatgttccac
     9301 ctcgcgctca tcctgcggat gttctgccgc aagagatgga taaactgcgt gagatgatgc
     9361 tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt atatcccagt
     9421 cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat gaaatctacg
     9481 atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg
     9541 gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg gcactggatg
     9601 cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg gaagatccgt
     9661 cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc gaagggtaag
     9721 tttggattta agccggatgc ggcgctgatt gcgccttatc gtgcctacat gcactgaacg
     9781 cctgtaggca cgataagtat gttgaccagt atcaatataa cggtttcatc ccggtaattg
     9841 gcagtccaca tacgcatcgg gcaatcacga cttccgttgc gccgttaact cggcaatacg
     9901 gacaatcacc tgcaccgctt tttccatacc ttccagagtc acaaactcat gcttaccatg
     9961 atagttgtaa ccgccagtga acaggttcgg gcacggtaat cccataaacg acaactgcgc
    10021 gccgtcggta ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc
    10081 ctgctgggcg atatcgataa tatgcggatg ctcaaccact ttctcgcgca tattgtagta
    10141 actgtcttca atcaccagtt caatgtagca atcaggatgt aaccctttgc ccactttttt
    10201 ggcgatctcc atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat
    10261 gatgtagtgc atatcggctc ggtcaacggt gcctttcatg ctcgccaggt gatagaaacc
    10321 ttcatagcct tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc
    10381 cgccagcgac agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc
    10441 gacaattttg atattgaccg atgcggcgtt gaagttttca aactccagtt cacctacgcc
    10501 accaccgtca acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc
    10561 ccctttgccc acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt
    10621 tttctgttgc aataccgcca gcgcggtcat gatttctgca ataccggctt tgtcatcggc
    10681 acctaacaag gttttaccat cggtagtaat cagcgtctga cccagtagct gatgcagcac
    10741 cgggaacata accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg
    10801 atagttttca acaatttgcg gattcacatt tttgccgctg caatccggtg aggtatccac
    10861 atgagaaata aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat
    10921 caaagtgccc ttctcactta aggtcacatt gataagcccc atctcttcga gctgctcttt
    10981 cagcagatgc aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt
    11041 tgattgggta tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc
    11101 accctcactt tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt
    11161 cacttttacc cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa
    11221 gccagtaatt cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc
    11281 attaagagtc accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt
    11341 tacatgggac agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa
    11401 ttggcgggaa ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact
    11461 atcaacaatg gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt
    11521 cttcgcctga ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag
    11581 gacatcaccc acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctacgca
    11641 cttttccccc acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc
    11701 cccgctgctg aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca actggaaacc
    11761 ttcgctcaac gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct
    11821 cgcgcggtgg ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat
    11881 tacaaactgc gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggtatt
    11941 accttcgtct ttgtcactca cgatcaggaa gaagcgctca ccatgtcaga caggattgtg
    12001 gtgatgcgcg agggtcgtat tgagcaagat ggtacaccgc gcgaaattta cgaagagccg
    12061 aaaaacctgt ttgttgccgg cttcattggc gaaatcaata tgtttaacgc cactgtcatc
    12121 gaacgtctag acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac
    12181 gttaacttcg ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta
    12241 cgtgttgaag agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag
    12301 cgtaactaca aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg
    12361 atggtcagcg aattcttcaa tgaagacgac cctgactttg accactctct cgaccaaaaa
    12421 atggccatta attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa
    12481 gttccagaat gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc
    12541 caacctgatg atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat
    12601 ggtctttacg ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt
    12661 gcactcgctg aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctacccgtt
    12721 tgcctggttt ctagcgaagc taccacacaa ggtgcgtccg ctgctgctgt ttctgctgat
    12781 tgttcccttc tggaccaact cactgattcg catctacggg ctgaaaattt tcctcagcac
    12841 caaaggctat ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caattcgtat
    12901 catgttcacg cccagtgcgg tgattatcgg tctggtttac attctgctac cgtttatggt
    12961 gatgccgctg tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga
    13021 tctcggtgcc agcaagttac agacttttat ccgtatcatt attccactga cgatgccggg
    13081 aattattgcc ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtatccga
    13141 cctgatgggc ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtcc agttcctcaa
    13201 tattcgtgac tggccgtttg gtgcagccac cagcattaca ctgactatcg taatgggcct
    13261 gatgttgctg gtttactggc gcgcttctcg tctgctgaat aaaaaggtgg aactcgaatg
    13321 atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca
    13381 atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt
    13441 tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag
    13501 cattcactaa caatggcggt gttttcggcg acatttgcta cgcttatcgg ttcactgacg
    13561 gcagttgcgc tgtaccgtta tcgctttcgt ggtaagccgt tcgttagcgg aatgctgttt
    13621 gtggtgatga tgtcaccaga tatcgtgatg gcgatttctc tgctggtgct gtttatgctg
    13681 ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgccg
    13741 tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg
    13801 gcgaaagatc tcggtgccag tgaatttacc attctgcgaa aaatcattct gccactggca
    13861 atgcctgcgg tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta
    13921 gtttcttcgt tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg
    13981 gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg
    14041 ctggtgatgg ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg
    14101 gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca
    14161 tgagcgccgc tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg
    14221 tgccgccagg actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga
    14281 cttacgagtc gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg
    14341 acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagaaggg atgatccaga
    14401 agatcgacaa gtcgaagtta agcaatttca gcaacctcga tccagacatg ctcaacaagc
    14461 cgtttgaccc gaataacgac tactccattc cgtatatctg gggggcgacg gcgattggcg
    14521 ttaacggtga tgcggtagat ccgaaatccg ttaccagctg ggccgatctg tggaagcctg
    14581 agtacaaagg tagcctgctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc
    14641 gtaagctggg ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg
    14701 agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt
    14761 acatggaagg cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc
    14821 aggcgggtac gcctattgac gtggtgtggc cgaaagaagg cggcattttc tggatggaca
    14881 gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc
    14941 tgctgcgccc ggatgtggca aaacaggttg ccgaaactat cggttatcca acgccaaacc
    15001 ttgcggcgcg taagctgtta agcccggaag tggcgaacga taaaacgctc tacccggatg
    15061 ctgaaaccat taaaaatggt gaatggcaga atgacgttgg cgcagccagc agcatttatg
    15121 aagagtatta tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggcattac
    15181 cgcccgtttt ttatttagca aggatgtggc taatgagtca ggacagtaaa gtgttatccc
    15241 gcgcttttct cggtatcggt ctgatattgc tcgttatttc tgcaatcatc ttttattacc
    15301 attttacttt taccaaaagc gcggttcata cagaaggaat cattgttgat gccgtttggt
    15361 acaacaatca ctcaaacgac gtcgatgata atggttcctg gtatccagtg gttgcctttc
    15421 gccctacgcc agattacacg ctaattttca attcgaatat cggtagtgat ttttatgaag
    15481 atagcgaagg tgacagagtc aatgtctatt atccgccagg gcatccagaa caagccgaaa
    15541 taaacaatcc ctgggttaat ttttttaaat ggggctttgt tggcatagca ggcattattt
    15601 tcggtagtgt cggtctgata atcacgctcc cctccaccaa aaaatctcgc aggaaacgga
    15661 agtcccgccc atgaaaaaat tcattatact gctcagttta ctgatactcc tgcctctggt
    15721 agcaaccagt aaaccattga ttccaataat gaagacactt tttacggatg ttaccggtac
    15781 tgtaccggat gctgaagaga tcgcacgtaa agccgagctt tttcgccaac aaacgggaat
    15841 tgcaccgttt attgtgattt tacccgacat taataatgaa gccagtctca gacaaaatgg
    15901 taaagcgatg ctggcgcatg cgtcatcttc attgagtaat gtaaaaggaa gtgttctgtt
    15961 actatttact acccgcgaac cacggttaat tatgatcacc aacggccagg ttgaaagtgg
    16021 tctggacgat aaacatctcg gccttctgat agaaaatcac acgctggctt atttaaatgc
    16081 agatctctgg tatcagggaa tcaataatgc attggctgtt ctacaagcac agatattaaa
    16141 acaaccgacg ccgccactga cgtattatcc gcatccagga caacagcatg aaaatgctcc
    16201 ccccggcagc accaacacat taggttttat cgcctgggct gcgacgttta tcttattttc
    16261 ctggatcttt tgctacacga ctcgttttat ttatgcgtta aaatttgcag tggcgatgac
    16321 tattgccaat atgggttatc aggctttatg tttgtatatc gataatagct ttgcgattac
    16381 cagaatatcg ccactctggg cagggctaat tggtgtctgt acgtttattg ccgcattgtt
    16441 attgacgagt aaacgataaa agaggccgga aaagtgcgcg tgtattatgc cggatggggc
    16501 gcgaacgcct tatccggccc acaaaacccg caaattcaat taattgcgtc cccttgtagg
    16561 cctgataagc gtagcgcatc aggcaatgct tcctgcttta taatcccttc agcaactttt
    16621 caacaaactc aggcaccacc tggcttgccg ggccgtaata tttctcggca aattcattac
    16681 cgacctgact tggttcgaga ttcagttcca cggtgtgcgc gccatgcagt ttcgcttcgt
    16741 gaacaaaccc agccgccgga taaacatgcc cggaagtacc aatggcaatg aaaatatcag
    16801 ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca aaccaaacga
    16861 cgtgcgggcg caagggtgcc gggaactggc aacaatggca tttatcttct ggcgtaacgt
    16921 ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg aactttcagc aattccccat
    16981 gcatatgaat gacattggta ttacctgcgc gttcatgcag gttgtctata ttctgcgtta
    17041 ccagcaaaaa gcgatcgccg agggcgtctt gcagtttagc cagcgcaaga tgcgcggcgt
    17101 taggctgaat ttctggctgc tgcagctgtc gacgacgggc gttataaaac gcttgcacca
    17161 gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc
    17221 acaggccatc tgcggcgcga aaggtacgaa tacctgattc cgcagaaatc cctgcccctg
    17281 tcagtacgag tactcttggt ttttccattg cttccggcac cactttatct ctgaaaaaaa
    17341 tacgctgacg caaacgctcg cgcaggcggc gtttattttt acgaaaacga cttaaccgat
    17401 gaccccgacg cgacagcata gcaacctctg ttgtttaatc ggttagatgt aggaaggccg
    17461 caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc aatgcgcgga acacgagcta
    17521 caggtaagag atgacgaggc agcctgtccg ccagttgcgt tgtgattgcc gggaaattcg
    17581 ataagccacc accaatgacg accaggtcag ggtcaacaat ggtcaggata tttcccagac
    17641 aaaccgctaa taaatccaga taacgctcaa cgtgcgccct tgcctgctca tcgccttgat
    17701 cataaagcgc aataatttcg ggagcctgca acggttgatg ataatagtgt tgatacagcc
    17761 acgcaaaacc gcgaccagac agataatttt caatgcagcc atgctgacca cagccgcagc
    17821 ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc aaccggcaga cgcatatggc
    17881 caaactcgcc ggtaatgtag cttttaccgg taatcggttt gccgttgaaa atcagcccgc
    17941 cgccaacgcc ggtgccgaga atcaacccca tcaccagtgg atattgagta aattcatcat
    18001 cccaggcttc tgaaagggca aaacagttgg catcgttatc aaggcgtaca tcgcgatcaa
    18061 gacgtgcgct caggtcggca cgcagcggtt taccgctggc agcggggaca ttggcggcat
    18121 acagcgtacc atcttctgtt tccggcattc ccggaatacc gatgccgaca gaacctttac
    18181 agccaaaacg ttggtcagct tcagctacca gctcacacac tgcatctaaa aatgcgtcat
    18241 agctgtcacg cggtgtcggc acccgctttt cccactgcaa ctgccgaccg ctatcaaaca
    18301 cgccaagcgc aatttttgtt ccaccaatat caaacccgta atacattgcc gctccttgtt
    18361 ttaatgtact gcctttactg gccgctaagg actcgcgcag ggtcaatatt actggcgcgc
    18421 cgcgccggat accaacttgc caaaagactc agcaacaatg ctgtgaccag tacgtagaag
    18481 acgtccagcc agtgcaattc cgatggcagg aaatcaataa aatagatatc gctggagagg
    18541 aactgatgac cgatcaactt ttcaatccac tcaataatcg gggtaagttg cagtgaaacc
    18601 accacaccga taatcacccc acacaggctg ccgaacagcc ctgccagcaa tccataccag
    18661 acaaagatgg cgcgaattaa accatctttc gcccccagcg ttcttaatac tgcgatatcg
    18721 ccactcttgt ctttcaccgc catcactaag gtggagacga tgttgaaaca ggccacgcca
    18781 atcaccagta ccatcgccag atacataatg gcgcggatca tctggatatc gcgatacata
    18841 tagccgtaag taccaatcca acttttaata taaacatagc tgttggtcac ttcacccgca
    18901 tcgcgtacca gcttattggc gttgaaaaca tccgtcattt taagggcaat acctgacacg
    18961 ctggaaccca tatcaagata ttgttgggca tccgccagcg ggatcatggc aaaactgtga
    19021 tcgagttgac cactcaactg caaaataccg gcaacgtgca aacgcacacg ttttggctgc
    19081 atcagtttat gctcgggatt cgagttgggg atcataatcg acacccaatc cccctgcttc
    19141 actttcagcg catccgccac gcctttgccg atgataattt gctgttcgcc cgctttaaaa
    19201 ttgcgccacg catcgccctg gacaaacgag ggtaatgcgc tcagacgctg ttcctgttgc
    19261 gggttaacgc ccttcacctg gattgcgcga agattcacgc cactttccac cagcccggtg
    19321 aaattgatat acggcgcggc agcggcaata cccggcactt tttgcacgtg atccagtgct
    19381 tcctgccagt tagtccacgg ctgatccacc gcctcgattt caccatgcgg caccaccgcc
    19441 agaatacggt tgttcagttc gcgttcaaag ccgttcatcg cgcttaagcc gacgatcaat
    19501 accgccacgc cgagggcaat gccaatggta gaaataacgg agatcagcga caccatgccg
    19561 ccgcgccgcc gtccgcgact aaaacgcagg ccaattaata acgataaagg catcgccatt
    19621 actccgcccc catcaggctc agttccgccg tcagacgacc atcacgcatt tctaactggc
    19681 ggctcatacg tttcgccagt tgcaggtcgt gagtaaccac caggaaggcg gtgccctgca
    19741 agcgattcaa ttccccaagc aactggaaga tgctgtcggc gttacgcgca tcgaggttac
    19801 cggtaggttc atccgccagt accaggcgcg ggttattcac cagcgcacgg gcaatcgcca
    19861 cacgctggcg ttcgccgcca gaaagttcag atgggcggtg attcgcacga tgatccagcc
    19921 ccaccgcttt taacatctca agtgcacggc tgttgatttc agcgggcttt ttcttgccaa
    19981 tcagcagcgg catagccacg ttttccaggg cggtaaaatc cggcagcagg tggtgaaact
    20041 gataaataaa gcccagcttc tggttgcgca gctctgcttt cgccgccgaa gacagtttgc
    20101 tcatcggttg accgttaaag atcacatcgc cggaggttgg cgtatccagc ccgcccaaca
    20161 agtgcagcaa ggtgctttta ccggaaccag agctaccgac gatcgccatc atttcacctt
    20221 cgccgacgct gaaactgaca ttgtgcagca catcggtttg cacactgcct tcctgatagc
    20281 gtttgcacag gttgtcgcat tgcaacagga tcttattcat aacgtaaagc ctcagcgggt
    20341 tgagtggcgg cagcgcgcca tgaagggtaa agcgtagaca gcagcgcgat agccatcgcc
    20401 accagcgcaa taacaatgac ctgtaaaggt tcgatagcga ccggcagcgc cgcgccatca
    20461 agcaggacgc cgattatcgg catcagatta tttaactggc tggcaagcag ggcgccaagc
    20521 gccgctccga ggatcgcacc gataatcccg gcgctggccc cttgcaccat aaagaccatc
    20581 atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg ctacttcgcc ctgcttctcc
    20641 attaccatca gccctagtga ggtaataatg ttaaacgccg caacggcgac aatcaggctc
    20701 agcagtaaac ccatcatatt tttttccatg cgtacggcct ggaacaactc gcctttacga
    20761 tcacgccagt cctgccattt gctgccttca ggcagttttt gctgacttaa tgagtcgact
    20821 ttcagcggct catccagcca caaacgccag ccggtaatat tgcctgccgg ataacgcatc
    20881 agacgcgagg catcctcaat attcaccagc atttcattgc catcgacttc actgttggcg
    20941 gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa tacgccccat cggcgtgaac
    21001 tggctggcag atggtaccat catgcggatt tgatcaccgc gattaacgcc tagctgtgag
    21061 gcaagttgtt cgccgaggat gacattatat ttccccggct cgaggtcagt ttgtttcaca
    21121 ttgaccagat acggcgttag tggatctttt tgcgccgggt cgataccgag catcaccccg
    21181 accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg tagtaatagg tgcgacgcga
    21241 ttaacgccgt ccagtttgac tgccgtttcc gggagttgct gcgggttaag agagccatgc
    21301 tcagaagaga gaattgcctg tggcatcagg ccaaggatgt tgttttgcag ctcgcgctca
    21361 aagccgttca tcactgacaa tactgtgacc agcgccatca ccccgagggt aatgccgatg
    21421 gtagaaagcc aggagacgaa acgaccgaag cgatccgctg cacgcccacg catgtaacgc
    21481 aggccaataa atagagcgac aggttggtac atgaaatccg tctggttgct gtagcaaaga
    21541 cacgagtata taagcgaaag caagatgggt aaacgtctga actgtaagtg atcgtcaggg
    21601 ctaatctgaa aagcccccgt gaatcaatcg gctaaattgc catgttttat catcaatctt
    21661 gccgcctgat cttaatctga aaaatcatcc aatacagaca gatacccatt acacataggg
    21721 tcataacccg caggatcaac cgcatgtaaa ttaaaaaaga gcgtgttgtc ctgatgaaac
    21781 aaaaagagct atggattaac cagatcaaag ggttatgtat ttgtctggtg gtgatttatc
    21841 actcggtcat taccttttat ccgcatctga ccacttttca gcatccgtta tcggaagtcc
    21901 tgagcaaatg ctggatctat ttcaatcttt accttgcacc ctttcgtatg ccggtttttt
    21961 tctttatttc cggctatttg attcgccgct atatcgacag cgtaccgtgg ggaaattgtc
    22021 tcgataaacg catctggagc atcttttggg tgctgacgct ttggggcgtg gtgcagtggc
    22081 tggcgttaag tgcgcttaac cagtggctgg cacctgagcg cgatttaagc aacgcttcca
    22141 atgccgcgta tgccgattcc accggtgagt tcctgcacgg gatgatcaca gccagcacca
    22201 gcttgtggta tctgtatgcg ttaattgtct atttcgtggt atgtaaaatt tttagccgtc
    22261 tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca
    22321 cgccgtggtg gggaatgaac agtgtgatcc gcaatttgcc ttattacagc cttggcgcat
    22381 ggtttggcgc aacattaatg acctgtgtta aagaggtgcc gttgcgccgc catctgctga
    22441 tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt
    22501 tgtcgctggt atcgattgtg gtgatcatga agctgtttta tcaatacgag caacaattcg
    22561 gtatgcgctc caccagcctg ctgaatgtga ttggctccaa caccattgct atctacacca
    22621 cccatcgcat tctggttgaa atattcagct taactctgct tgcgcaaatg aacgcagcac
    22681 gctggtcgcc gcaagtcgaa ctgacactcc tgctgggtta cccctttgtt agtttgttca
    22741 tctgtactgt tgcgggcttg ctggtaagaa aactttcaca gcgcgcattc agcgatctgt
    22801 tgttctcccc gccttctctg cccgcggcca tcagttactc ccgctaagcc atcgccccag
    22861 ccggggcgat tcaatttgct aaaccatgtc gattacggat aatgaacagc attgcttatc
    22921 agagatatgc ccccatatgt tgaggcatat cctaacgaga atctgacaac cgttatgcct
    22981 gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta
    23041 accggcgctg cctgcgcgac gctggtagcg gaaattgccg aacgtcacgc cggtccggtg
    23101 gtactcattg caccagatat gcaaaatgct ctgcgtttgc atgatgaaat cagccagttt
    23161 accgatcaaa tggtgatgaa tctggcggac tgggaaactc ttccctacga cagtttttcg
    23221 cctcatcagg acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag
    23281 cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc gcacagtttt
    23341 cttcacggtc atgcgctggt gatgaaaaaa ggtcagcgtc tgtcacgaga tgcattaaga
    23401 acccaactgg acagcgccgg ttatcgccat gttgaccagg taatggagca cggcgaatac
    23461 gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt
    23521 gatttctttg atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg
    23581 ctggaggaag tagaagcgat caatctgctg cccgcgcacg aatttccgac cgataaagcg
    23641 gcaattgaac tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccggaa
    23701 catatttacc agcaagtgag taaaggcaca ttacctgccg ggatcgagta ctggcagcca
    23761 ttgttcttca gcgaaccact gccgccgctg ttcagttatt tccctgccaa taccttgctg
    23821 gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc aggctgacac gctggcgcgt
    23881 tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc caccacaatc gctctggctg
    23941 cgggtggaca agctcttctc agagctgaaa aactggcccc gggtgcagct aaaaactgaa
    24001 catttaccga caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc
    24061 gttcaggcac aacaaaaagc gccgctggat gcgctgcgta agttcctcga gactttcgac
    24121 ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc
    24181 gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg atgaagccag cgaccgtggg
    24241 cgttatctga tgattggcgc tgccgaacat ggttttgtcg ataaggtgcg taatctggcg
    24301 ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc gccgtcgtca ggattctcgc
    24361 cgcaccatca accccgatac actgatccgt aaccttgcgg agctgcatat tggtcagccg
    24421 gtggtccatc tggagcacgg tgtcgggcgc tatgccggaa tgaccacgct cgaagcgggc
    24481 ggcattactg gcgagtattt gatgctcacc tatgccaacg acgccaaact gtatgttccg
    24541 gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg cggaagaaaa cgccccgctg
    24601 cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga aagcggcgga aaaagtgcgt
    24661 gatgtggcgg cggaattgct ggatatctac gcgcaacgcg ccgccaaaga gggcttcgcg
    24721 tttaaacacg atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg
    24781 ccggatcagg cgcaggccat taatgcggta cttagcgaca tgtgtcagcc gctggcaatg
    24841 gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat gcgcgccgct
    24901 ttcctggcag tagataacca caagcaggtg gcggtgttgg tgcctaccac ccttctcgcg
    24961 cagcagcatt acgacaactt ccgcgaccgt ttcgccaact ggccggtacg tatcgaaatg
    25021 atctcccgtt tccgcagcgc caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg
    25081 aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa gtgacgtcaa gtttaaagat
    25141 ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgtatt
    25201 aaagcgatgc gcgcgaacgt ggatattctg acgcttactg caacgccgat cccacgtacg
    25261 ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt
    25321 cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc tggtggtccg ggaggcgatc
    25381 ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct acaatgatgt ggaaaacatt
    25441 cagaaagccg ccgaacggct ggcagaactg gtgccagaag cgcggatcgc catcggtcac
    25501 gggcagatgc gcgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc
    25561 aacgtgctgg tttgtacaac cattatcgaa accgggatcg acatcccgac agccaacacc
    25621 attatcattg aacgcgcgga tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc
    25681 gtcggacgtt cacatcatca ggcatatgca tggttgctga cgccgcatcc aaaagcgatg
    25741 actaccgatg cacaaaaacg tcttgaagcg attgcctcgc tggaagatct cggggcaggt
    25801 tttgcgctgg caacgcacga tctggagatt cgcggcgcgg gtgaactgct tggcgaagaa
    25861 caaagcggct caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc
    25921 gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca
    25981 gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt tcattcctga cgtgaatacg
    26041 cgtttgtcgt tctataaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc
    26101 aaagtcgagc ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac cctgctggat
    26161 gttgcccgtc tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag
    26221 aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt
    26281 ttgctgcaaa aacagccgca gcattaccgc cttgatggtc cgacgcgcct gaaatttatt
    26341 caggatttga gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg
    26401 gaagagaacg cgatcgctta atggtaaatc gggggcgttt ctgcgccccc atctgcaaca
    26461 tttacaaatt ctttgcactt ccctgcacta tccgacactg tcaccatcca taattcaggc
    26521 ttatctgttt attacaataa ccttatattt attatggatt ttttggtgat gatcaaaacg
    26581 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    26641 gcaaaagcca acacctggcc gctgccgcca gcgggcagtc gtctggttgg cgaaaacaaa
    26701 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    26761 ggctttctcg ctctgttaca ggctaaccct ggcgttgatc cttacgtacc gcgcgcgggt
    26821 agcgtgttaa cgatcccgtt gcaaacccta cttccagatg cgccgcgcga aggcattgtg
    26881 atcaatattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    26941 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    27001 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    27061 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    27121 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    27181 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    27241 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    27301 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    27361 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    27421 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    27481 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    27541 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    27601 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    27661 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    27721 gcgatcccag atttgtcccc gcgttagcac tgattgtacc gactttttgt tggccttctg
    27781 gcgttgactg aacttcgaca gccgcaaagc tggcaaatga catggagctt aaaatcgccg
    27841 cagcgatgag ggtttttacg tttttcataa tagtggcctt atgcagatga atgacgtcgc
    27901 aagcaattac ttaacgatcg ataactaa
//
LOCUS       DAZFVO020000057        26922 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFVO020000057 DAZFVO020000000
VERSION     DAZFVO020000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.057"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            110..517
                     /gene="gloA"
                     /locus_tag="ACHKNB_RS11155"
                     /old_locus_tag="pgaptmp_002231"
     CDS             110..517
                     /gene="gloA"
                     /locus_tag="ACHKNB_RS11155"
                     /old_locus_tag="pgaptmp_002231"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HGU4195734.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            620..1267
                     /gene="rnt"
                     /locus_tag="ACHKNB_RS11160"
                     /old_locus_tag="pgaptmp_002232"
     CDS             620..1267
                     /gene="rnt"
                     /locus_tag="ACHKNB_RS11160"
                     /old_locus_tag="pgaptmp_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HGU4195735.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            1360..5977
                     /locus_tag="ACHKNB_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /pseudo
     CDS             1360..5977
                     /locus_tag="ACHKNB_RS11165"
                     /old_locus_tag="pgaptmp_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(6028..6375)
                     /gene="grxD"
                     /locus_tag="ACHKNB_RS11170"
                     /old_locus_tag="pgaptmp_002234"
     CDS             complement(6028..6375)
                     /gene="grxD"
                     /locus_tag="ACHKNB_RS11170"
                     /old_locus_tag="pgaptmp_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HGU4195736.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            6710..7525
                     /gene="mepH"
                     /locus_tag="ACHKNB_RS11175"
                     /old_locus_tag="pgaptmp_002235"
     CDS             6710..7525
                     /gene="mepH"
                     /locus_tag="ACHKNB_RS11175"
                     /old_locus_tag="pgaptmp_002235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HGU4195737.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            7653..8234
                     /gene="sodB"
                     /locus_tag="ACHKNB_RS11180"
                     /old_locus_tag="pgaptmp_002236"
     CDS             7653..8234
                     /gene="sodB"
                     /locus_tag="ACHKNB_RS11180"
                     /old_locus_tag="pgaptmp_002236"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HGU4195738.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(8380..9549)
                     /locus_tag="ACHKNB_RS11185"
                     /old_locus_tag="pgaptmp_002237"
     CDS             complement(8380..9549)
                     /locus_tag="ACHKNB_RS11185"
                     /old_locus_tag="pgaptmp_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4195739.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(9715..9804)
                     /gene="cydH"
                     /locus_tag="ACHKNB_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /gene_synonym="ynhF"
     CDS             complement(9715..9804)
                     /gene="cydH"
                     /locus_tag="ACHKNB_RS11190"
                     /old_locus_tag="pgaptmp_002238"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HGU4195740.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            10103..11128
                     /gene="purR"
                     /locus_tag="ACHKNB_RS11195"
                     /old_locus_tag="pgaptmp_002239"
     CDS             10103..11128
                     /gene="purR"
                     /locus_tag="ACHKNB_RS11195"
                     /old_locus_tag="pgaptmp_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HGU4195741.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(11125..12057)
                     /gene="punR"
                     /locus_tag="ACHKNB_RS11200"
                     /old_locus_tag="pgaptmp_002240"
     CDS             complement(11125..12057)
                     /gene="punR"
                     /locus_tag="ACHKNB_RS11200"
                     /old_locus_tag="pgaptmp_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HGU4195742.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            12170..13381
                     /gene="punC"
                     /locus_tag="ACHKNB_RS11205"
                     /old_locus_tag="pgaptmp_002241"
     CDS             12170..13381
                     /gene="punC"
                     /locus_tag="ACHKNB_RS11205"
                     /old_locus_tag="pgaptmp_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HGU4195743.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            13672..14820
                     /gene="cfa"
                     /locus_tag="ACHKNB_RS11210"
                     /old_locus_tag="pgaptmp_002242"
     CDS             13672..14820
                     /gene="cfa"
                     /locus_tag="ACHKNB_RS11210"
                     /old_locus_tag="pgaptmp_002242"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HGU4195744.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(14860..15501)
                     /gene="ribE"
                     /locus_tag="ACHKNB_RS11215"
                     /old_locus_tag="pgaptmp_002243"
     CDS             complement(14860..15501)
                     /gene="ribE"
                     /locus_tag="ACHKNB_RS11215"
                     /old_locus_tag="pgaptmp_002243"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGU4195745.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            15716..17089
                     /gene="mdtK"
                     /locus_tag="ACHKNB_RS11220"
                     /old_locus_tag="pgaptmp_002244"
     CDS             15716..17089
                     /gene="mdtK"
                     /locus_tag="ACHKNB_RS11220"
                     /old_locus_tag="pgaptmp_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HGU4195746.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(17130..18386)
                     /locus_tag="ACHKNB_RS11225"
                     /old_locus_tag="pgaptmp_002245"
     CDS             complement(17130..18386)
                     /locus_tag="ACHKNB_RS11225"
                     /old_locus_tag="pgaptmp_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4195747.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            18694..18770
                     /locus_tag="ACHKNB_RS11230"
                     /old_locus_tag="pgaptmp_002246"
     tRNA            18694..18770
                     /locus_tag="ACHKNB_RS11230"
                     /old_locus_tag="pgaptmp_002246"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18728..18730,aa:Val,seq:gac)
     gene            18775..18851
                     /locus_tag="ACHKNB_RS11235"
                     /old_locus_tag="pgaptmp_002247"
     tRNA            18775..18851
                     /locus_tag="ACHKNB_RS11235"
                     /old_locus_tag="pgaptmp_002247"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18809..18811,aa:Val,seq:gac)
     gene            18959..19264
                     /locus_tag="ACHKNB_RS11240"
                     /old_locus_tag="pgaptmp_002248"
     CDS             18959..19264
                     /locus_tag="ACHKNB_RS11240"
                     /old_locus_tag="pgaptmp_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HGU4195748.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTVRLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            19390..20994
                     /locus_tag="ACHKNB_RS11245"
                     /old_locus_tag="pgaptmp_002249"
     CDS             19390..20994
                     /locus_tag="ACHKNB_RS11245"
                     /old_locus_tag="pgaptmp_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HGU4195749.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(21006..21818)
                     /gene="ydhT"
                     /locus_tag="ACHKNB_RS11250"
                     /old_locus_tag="pgaptmp_002250"
     CDS             complement(21006..21818)
                     /gene="ydhT"
                     /locus_tag="ACHKNB_RS11250"
                     /old_locus_tag="pgaptmp_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HGU4195750.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(21822..22607)
                     /gene="phsC"
                     /locus_tag="ACHKNB_RS11255"
                     /old_locus_tag="pgaptmp_002251"
     CDS             complement(21822..22607)
                     /gene="phsC"
                     /locus_tag="ACHKNB_RS11255"
                     /old_locus_tag="pgaptmp_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HGU4195751.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFAPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(22604..23272)
                     /locus_tag="ACHKNB_RS11260"
                     /old_locus_tag="pgaptmp_002252"
     CDS             complement(22604..23272)
                     /locus_tag="ACHKNB_RS11260"
                     /old_locus_tag="pgaptmp_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HGU4195752.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(23336..23974)
                     /locus_tag="ACHKNB_RS11265"
                     /old_locus_tag="pgaptmp_002253"
     CDS             complement(23336..23974)
                     /locus_tag="ACHKNB_RS11265"
                     /old_locus_tag="pgaptmp_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HGU4195753.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(23987..26089)
                     /locus_tag="ACHKNB_RS11270"
                     /old_locus_tag="pgaptmp_002254"
     CDS             complement(23987..26089)
                     /locus_tag="ACHKNB_RS11270"
                     /old_locus_tag="pgaptmp_002254"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HGU4195754.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSGNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(26110..26736)
                     /locus_tag="ACHKNB_RS11275"
                     /old_locus_tag="pgaptmp_002255"
     CDS             complement(26110..26736)
                     /locus_tag="ACHKNB_RS11275"
                     /old_locus_tag="pgaptmp_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HGU4195755.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         6522 a         7105 c         6584 g         6711 t
ORIGIN      
        1 aaggctatac cgattacccg acgttgtaat ccaacattgc gagcggcgta aagccgccgc
       61 tatactaaaa caacattttg aatctgttag ccattttgag gataaaaaga tgcgtcttct
      121 tcataccatg ctgcgcgttg gcgatctgca acgctccatc gatttttata ccaaagtgct
      181 gggcatgaaa ctgctgcgta ccagcgaaaa cccggaatac aaatactcac tggcgtttgt
      241 tggctacggc ccggaaaccg aagaagcggt gattgaactg acctacaact ggggcgtgga
      301 taaatacgaa ctcggcactg cttatggtca catcgcgctt agcgtagata acgccgctga
      361 agcgtgcgaa aaaatccgtc aaaacggggg taacgtgacc cgtgaagcgg gtccggtaaa
      421 aggcggtact acggttatcg cgtttgtgga agatccggac ggttacaaaa ttgagttaat
      481 cgaagagaaa gacgccggtc gcggtctggg caactaatct cctgccgggc gtgaactcat
      541 cgcgcccgca tctttactgc atcgacaagt aatatttgtc ataatgcgcg ctgcaattta
      601 tccgtattaa gagaatcaga tgtccgataa cgctcaactt accggtctgt gcgaccgttt
      661 tcgtggtttt tatcctgttg tgatcgatgt tgaaacagcc ggatttaacg ccaaaaccga
      721 tgcgctgctt gagattgccg ccatcaccct gaaaatggat gaacaaggct ggctgatgcc
      781 ggacaccaca ttacatttcc acgtcgaacc atttgtcggc gcaaatttgc agccagaggc
      841 cctcgccttc aacggcattg acccgaacga tcccgatcgc ggcgcggtca gcgaatacga
      901 ggcgctgcac gaaattttta aagttgtacg taaaggtatt aaagcgagcg gctgtaaccg
      961 cgccattatg gtggcgcaca atgccaattt tgatcacagc tttatgatgg ccgccgcaga
     1021 acgcgcctca ctgaaacgta acccgttcca ccctttcgcc acttttgaca ctgctgcact
     1081 ggccgggctg gcactcggac aaaccgtatt gtcaaaggct tgccagaccg ctggcatgga
     1141 cttcgacagc acccaggcgc actccgcgct gtacgacacc gaacgcactg ctgtgctgtt
     1201 ttgtgaaatc gtcaaccgct ggaaacgtct gggaggctgg ccgctacctg ccgccgaaga
     1261 ggtgtaatcg agtcgatgcc tgatgacatg caatgattca ggcatctata gtgaggctat
     1321 tccacgcatc aagcatgata ttcacgggga atagcgttaa tggcagataa tccagaccct
     1381 tcatcgctcc tgccggacgt gttttcaccg gcgatccgcg actggtttct tcgcgccttt
     1441 aaacagccga ccgctgtcca gtcgcaaacc tggcatgtgg cggcgcgaag cgaacatgcg
     1501 ctggtgattg caccgaccgg ctccgggaaa acgctggcag catttctcta cgccctcgat
     1561 cggctcttcc gcgaaggcgg cgaagatacc cgcgaggcgc ataagcgtaa aacctcacgc
     1621 atcctctata tttcaccgat aaaagctctg ggcaccgacg ttcagcgcaa cttgcagctc
     1681 ccgttgaagg gtattgccga tgaacggcgg cggcgcggcg aaacggaagt caatcttcgc
     1741 gtagggatcc gtactggcga tacgcctgca caggaacgca gcaaactcac ccgtaatccg
     1801 ccggatattc tgatcaccac acccgaatca ctctatctga tgctgacctc ccgcgcgcgc
     1861 gaaacgctac gcggcgtcga aacggtaatt attgatgaag tccacgcggt agcgggcagt
     1921 aaacgtggtg cgcatctggc gttaagtctg gagcggctcg atgcgctgct ccacacctca
     1981 gcacagcgaa ttggcctttc tgccactgtg cgctcagcca gcgatgtggc agcatttctt
     2041 ggtggcgatc gcccggttac ggtagtcaac ccgcccgcaa tgcgccatcc gcagatacga
     2101 attgtcgtac cggtcgccaa tatggatgat gtctcatcgg tcgccagcgg caccggcgaa
     2161 gacagccatg ccggccggga aggctccatc tggccatata ttgaaacggg tatccttgat
     2221 gaagtgttgc gccatcgctc gaccattgtc tttactaatt cacgtgggct ggcggaaaaa
     2281 ctgacggcac gattaaatga gctttacgcc gcacgcttac agcgttcccc gtctatcgcc
     2341 gttgatacgg cccatttcga gtcgacctcc ggcgcaacct ctaaccatgt acaaagtagc
     2401 gacgttttta ttgcccgctc acaccacggc tccgtctcta aagaacaacg agcaatcacc
     2461 gaacaggcgc tgaaatcggg tgaattacgc tgcgtggtcg caacctccag tctcgaactg
     2521 gggattgata tgggcgcggt ggatctggtg attcaggtgg caacgccgct ttctgttgcc
     2581 agtgggttac aacgcattgg tcgcgccgga catcaggttg gcggtgtatc taaagggctg
     2641 tttttccctc gtacccggcg tgacttagtc gattccgcag tcattgtaga gtgtatgttc
     2701 gcaggcaggc tggaaaacct gacaccgccg cataatccac tcgacgtcct tgcgcagcaa
     2761 accgttgccg ccgcggcgat ggatgcatta caggtagacg aatggtactc ccgcgtacgc
     2821 cgtgccgcac cgtggaaaga tctgccaaga cgtgtttttg acgccacgct ggatatgctt
     2881 tccgggcgct atccctctgg cgatttttct gcttttcgcc ccaaactggt ctggaacagg
     2941 gagaccggga tattgaccgc ccgacctggc gctcaattgt tggcggttac cagcggcggc
     3001 accattccgg atcgtggcat gtatagcgtg ttattacccg aaggtgaaga acaggccggt
     3061 tcgcggcggg tgggtgaact ggatgaggag atggtatatg agtcgcgggt gaacgacatt
     3121 atcactctcg gcgctacctc atggcggatc cagcaaatca cccgcgatca ggtgattgtg
     3181 actcctgctc cgggtcgttc tgcccggctc cccttctggc gtggtgaagg taacggacgt
     3241 ccggctgaat taggcgagat gatcggcgat tttcttcatt tactggcgga tggcgcgttc
     3301 ttttccggga ctattccccc gtggctggca gaagaaaata cgaacgccaa tattcagggg
     3361 ttgattgacg agcaacgcaa cgcgacgggc atcgttccgg ggagtcgcca tctggtcctc
     3421 gaacggtgcc gtgatgaaat tggcgactgg cgtattattt tgcactctcc ctatggaaga
     3481 cgggtgcatg aaccctgggc gctggcgatt gccgggcgaa tacatgccct atggggcgct
     3541 gacgcgtctg tggtcgccag tgatgacggc attgttgcgc gcattcctga cactgatggt
     3601 aaattgcccg acgccgcgat ttttttgttt gaaccagaaa agttgctgca aattgtccgc
     3661 gaggcggtag gcagctcggc acttttcgcc gcccgttttc gcgaatgcgc cgcgcgggca
     3721 ttattaatgc cggggcgcac tccgggccat cgcacccccg ctttggcaac aacggctgcg
     3781 cgccagtcag ttgctggaaa tcgctcaggg atatccggat tttccggtca ttctcgaaac
     3841 cctacgcgaa tgtctgcaag atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg
     3901 cctgaacggt ggcgaaattc aaatatccga tgtaacgacc actacgccct cgcctttcgc
     3961 cacaagttta ttgttcggct atgtcgcgga atttatgtac cagagcgacg ccccgctggc
     4021 agagcgccgg gcatccgtac tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca
     4081 ggtcgatccg ggggaattac tcgacccgca ggtcattcgc caggtggaag aagagttgca
     4141 acgactggct cctggcagaa gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga
     4201 actggggcca atgaccgttg aagacctggc gcaacggcat acaggcagca gtgaagaggt
     4261 tgcgtcgtat ctggaaaatc ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg
     4321 acaggagcgt cttgcctgta tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg
     4381 actaccagag tcattgccag agatttattt acatagagtc agttacccgc ttcgcgatct
     4441 ctttctgcgc tatctccggg ctcatgctct ggtcacggct gaacaactgg ctcatgagtt
     4501 tagtctcggt attgccattg tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt
     4561 gatgaatctg caacaagaca tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg
     4621 ctcgctgcaa gccgccagag aagcgacgcg tcccgttgca gccacgacct atgcgcgatt
     4681 gctgctggaa cgtcagggcg tattacccgc caccgatggt agcccggcgc tctttgcctc
     4741 aacatcgcca ggcgtttatg agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc
     4801 cggagtcggt ttacccgcct cactctggga aagccagatc ctgcctgccc gcgtacgcga
     4861 ctattcgtca gaaatgctcg atgaattact ggcaaccggt gcggttatct ggtcggggca
     4921 aaaaaaactg ggtgaagatg acggcctggt ggcactgcat ctacaggaat atgctgcaga
     4981 atcgttcact cccgccgaag cggatcaggc gaatcgttcg gcgctgcaac aagcgatagt
     5041 cgctgttctg gctgacggag gagcctggtt tgcacaacaa atcagccaac ggatacgcga
     5101 caaaatcggc gaatcggttg atctctctgc cctgcaagag gcgttatggg cgctggtctg
     5161 gcaaggcgtc atcaccagcg acatttgggc accgttacgc gccctcaccc gcagcagttc
     5221 caacgcacgc acctcaactc gccgcagtca ccgggctcgt cgtggacgtc ctgtctatgc
     5281 gcaacccgtc tcgccgctgg tatcttacaa cacaccaaat ctggctggac gctggtcgtt
     5341 attgcaggtg gagccactaa acgataccga aaggatgctg gcgctggcgg aaaatatgct
     5401 cgaccgctac ggcatcatca gtcgtcaggc ggtgatagcc gaaaatatcc ctggcgggtt
     5461 tccatcgatg caaacgcttt gtcgaagtat ggaagactcc gggcgaatta tgcgaggtcg
     5521 ttttgtagaa ggtctgggtg gcgcgcaatt cgctgaacgt ctgactattg accgattgcg
     5581 cgatctggcg acacaagcca cgcaaacgcg ccactataca ccagtggcgc tctctgccaa
     5641 cgatccggct aatgtgtggg gaaatcttct gccctggcct gcacatccgg caacgctggt
     5701 tccaacgcgt cgggcgggtg cgctggtggt cgtttctggc ggcaaattgt tactctatct
     5761 ggcgcaaggt ggcaaaaaaa tgctggtctg gcaggaaaaa gaggaattac tcgccccaga
     5821 ggttttccac gcgctgacta ccgcactgcg tcgcgaacca cggctgcgct ttacgctaac
     5881 agaagtgaat gatctaccgg tccggcaaac gccgatgttt acgctgctgc gtgaggcggg
     5941 attttcaagt tcgccacaag ggctggattg gggatagaga aaggactgac ggatgcccgt
     6001 tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag acttgtattt
     6061 agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt cgatcacgat
     6121 atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg gccagttagc
     6181 atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat aggcaaaacg
     6241 ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc tcggcagttt
     6301 cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt ggattttttc
     6361 gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat ctgacattgt
     6421 agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta ttttatccct
     6481 ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc aaactggcgt
     6541 atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat
     6601 tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt
     6661 gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat
     6721 aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat
     6781 ggcccatgct tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc
     6841 agataaaaag aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc
     6901 cagtaaaagt acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc
     6961 ttctaaaaac gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt
     7021 taccgaaaag tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc
     7081 cgcgtcagga actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc
     7141 aatgaataaa ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg
     7201 taccggtttt gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg
     7261 tattccgcgt acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag
     7321 tgaactgaaa aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca
     7381 tgtcggcgtg tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat
     7441 tcaaatcact tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt
     7501 aatgacccca aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt
     7561 catcttttgt ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta
     7621 cgtatgcaaa ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat
     7681 atgctaaaga tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca
     7741 agcatcatca gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag
     7801 gtaaatcact ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc
     7861 aggtctggaa ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga
     7921 ctggaaaagt cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc
     7981 agtttactga tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca
     8041 gcgatggcaa actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg
     8101 cgactccgct gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg
     8161 cacgtcctgg ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa
     8221 atctcgctgc ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat
     8281 gccaacgcgg atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa
     8341 ctgtaacgca gaaggttatc cttctgcgtt tttgtttaat tagctgttag caacgcaaac
     8401 tgtttcaggt tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat
     8461 cgctcccatc accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc
     8521 agctgctccc agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc
     8581 cggcgcttca ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt
     8641 tgctgcgccc cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg
     8701 gattgccagc ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga
     8761 acgatctgcc agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac
     8821 cagcattgcc gtgacgaaca ctggtgttgc gtgggtaata ctttgtagta ccggagagat
     8881 ataggtgtag agagtaaaca ttgcaccagc tcccagtacc gtcgtcagca atgcagacag
     8941 cacctgcgga cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgcacc
     9001 acctttaggt aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc
     9061 cagaaatgac atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc
     9121 gatatttgcc agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt
     9181 tggcaccacg cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct
     9241 ggtcaaaatg cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt
     9301 gccgagcgtg aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag
     9361 tagaagcgtc atcagcggcg cgccaaccat tacgccaact gcataggcac tgattaacat
     9421 tccggcagcg ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg
     9481 cgagaactcc gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt
     9541 aattttcatg ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga
     9601 aagcatgaca gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt
     9661 ttgctaacaa acaggaagga gatgcgaggg agaacgcgct ccctcgagtg gaaatcagtg
     9721 cagcgcggca gtcaaaccca cggccacgat caaaccgagg acgataatcg ttgttaccag
     9781 tgaaaattta aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaatagtg
     9841 atattacgca tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg
     9901 tttttaccac ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg
     9961 tactggccat tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt
    10021 ttgttgtgcg taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag
    10081 ttagggtctg gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt
    10141 ttccactaca actgtgtcac acgtaatcaa caaaacacgt ttcgtcgctg aagaaacgcg
    10201 caacgccgtg tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag
    10261 cctgaaggtt aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta
    10321 ttttgccgag atcattgaag cggttgaaaa aaattgcttc cagaaaggtt acaccctgat
    10381 tctgggtaat gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca
    10441 aaaacgcgtc gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat
    10501 gctggaagag tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga
    10561 cttcaccgat gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct
    10621 gattgaacgc ggtcaccgcg aaatcggcgt catccccggc ccgctggaac gtaacaccgg
    10681 cgcaggccgc cttgccggtt ttatgaaggc gatggaagaa gcgatgatca aggtgccgga
    10741 aagctggatt gttcagggtg actttgaacc cgaatctggt tatcgcgcca tgcagcaaat
    10801 actgtcgcag ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg
    10861 cgcactttgt gctgctgatg aaatgggtct gcgcgtcccg caggatgttt cgctgatcgg
    10921 ttatgataac gtgcgcaacg cccgctattt tacgccggcg ctgaccacga tccaccagcc
    10981 aaaagattcg ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg
    11041 tgaagaaccg cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga
    11101 cggcccgttc cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg
    11161 cagccactcc ttattcagcg tctcactatc gccgagatac tcaagcagcc aggttaacgc
    11221 aggcgacata tcattttgct gccatgtcag acaacatgcc gaatccggga aggggttttc
    11281 caattcaagt gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac
    11341 catccctatg cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac
    11401 aactcttttt tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc
    11461 ttcgcgcacc aacgacggcc agttgcgcaa cgtatcatcg ctgaatgggc catccatcaa
    11521 cgccagcggg tggtggctgg caacaacgca actccagctt agcatcccca tatcccggaa
    11581 ggcataacga ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc
    11641 gtcggaaagc gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc
    11701 atcaaaatgg cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt
    11761 atccactgcg atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg
    11821 ggtgatctgc atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc
    11881 ggtcagctcc acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag
    11941 ctgacgcacg gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct
    12001 aaaactacca ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt
    12061 ctgcctgcaa aatttttgaa accagtcatc aaatattacc gtttcacaac actaatttca
    12121 ctccctacac tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg
    12181 gaaaagattt ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat
    12241 gtatctgcct gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag
    12301 tgccagcctt agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccact
    12361 ctccgaccgt tatggtcgta aaccggtgtt attaatcggc ctgacaattt ttgcgttagg
    12421 tagtctgggg atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca
    12481 ggctgtgggt gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc
    12541 ttcacagaaa gttaaccgaa tttttgcgac catcatgccg ctggtgggtc tatctccggc
    12601 cctggctcct ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc
    12661 caccctgttt gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac
    12721 gaaggcccgt aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac
    12781 ctatcgcggc aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac
    12841 cggttcaccg ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta
    12901 tgtcccgcaa actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa
    12961 atggcaaggc aagcagttat taccgtggtt gctggtgctg tttgcagtca gcgtcattgc
    13021 gacctgggct gcaggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg
    13081 tgtgatggcg attgccaatg gcgcgatcta cccgattgtt gtcgcccagg cgctgcgtcc
    13141 cttcccacac gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg
    13201 cttcctcgca agtctggtag tttcctggct gatcagtatc agcacgccat tgctcaccac
    13261 caccagcgtg atgttatcaa cagtagtgct ggtcgctctg ggttacatga tgcaacgttg
    13321 tgaagaagtt ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg
    13381 atctatatcg atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt
    13441 agcggcctat actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga
    13501 taggatcgtt cccgactcac tatggatggt catttcggca aaggttcctc ctttccctct
    13561 gttctacgtc ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa
    13621 agcgtctacg ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca
    13681 tcgtgtatag aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta
    13741 cttagccgtg ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac
    13801 cccgattttt ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg
    13861 gatggctggt gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt
    13921 ctcgagaacc aactccccca tcatttcaaa gacacgctgc gtattgccag cgctcgtctc
    13981 ttcaatctgc agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt
    14041 aatgacttgt tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa
    14101 gatgccgata atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg
    14161 cagttaaaac cagggatgcg cgtactggat attggctgcg gctggggcgg actggcgcac
    14221 tacatggcat ctaactatga cgtaagcgtg gtcggcgtca ccatttctgc cgaacagcaa
    14281 aaaatggctc aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt
    14341 gacctgaacg accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg
    14401 aaaaattacg atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc
    14461 ctgctccata ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa
    14521 tatatttttc cgaacggttg cctgccctct gtacgccaga ttgctcaatc cagcgaaccc
    14581 cacttcgtga tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg
    14641 tggtatgaac gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt
    14701 aaacgaatgt ttacctatta tctgaatgcc tgtgcaggtg ctttccgcgc ccgtgatatt
    14761 cagctctggc aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa
    14821 aggctattct atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg
    14881 attcatggca ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt
    14941 ttgtggatca atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg
    15001 ttccagagtt tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt
    15061 caggctaata ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact
    15121 atcctggact ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc
    15181 agcggtggtc ataatatgac ctgacattaa gtgtccgcca atttcatcac tgaatttcgc
    15241 cgcacgctca acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa
    15301 cgtttctttc atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca
    15361 gcaaccgtta tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa
    15421 ctccaccaca tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc
    15481 ctgtacaatc cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag
    15541 cacaatagca ggtggcaaac gcccttacca gtgaaggggt aagaatgact attttttcac
    15601 tggagaatta ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct
    15661 gcccattcag gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca
    15721 gaagtatatc agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca
    15781 aatcgcccaa actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac
    15841 cgacatggcg gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca
    15901 cggactgctg ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga
    15961 gcgcattgcg catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat
    16021 tatgcttgtg ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc
    16081 tctggcggac aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc cgggctatct
    16141 gttcttccag gttgcccgta accaatgtga aggtctggca aaaaccaagc cgggtatggt
    16201 aatgggcttt atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca
    16261 tttcggtatg cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg
    16321 ggtcatgttc cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatattcg
    16381 taacgaaaaa ggcaccgcga aacccgatcc tgcggttatg aaacgactga ttcaactcgg
    16441 tttgccgatt gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt
    16501 agtgtctccg ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc
    16561 actgatgttt gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg
    16621 tctgggtcag ggctcaacgc tggatgcgca aaccgctgcg cggaccgggc ttatggtggg
    16681 tgtctgtatg gcaaccctga cggccatatt cacggtttca ctgcgggagc aaatcgccct
    16741 gttgtacaac gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc
    16801 ggtatatcag atttctgact caatccaggt gattggcagt gggattttgc gtggttataa
    16861 agatacgcgt tccattttct atatcacctt tacggcttac tgggtgctgg gcttgccaag
    16921 cggctatatt ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg
    16981 gataggcttt attattggcc tgacgtcggc agccattatg atgatgctgc gtatgcggtt
    17041 cctgcaacgt ctgccgtcag ttatcattct gcaacgagca tcccgctaat aaagacaagg
    17101 cgcaaccttc acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg
    17161 tcttcgtctg gctgtaattt cagagcgttg cacagagttt gccgactggc atctgttatc
    17221 tcaacgtgta tatccccggt aacttcccct ttcacattga tgaagtcagg tgagatttct
    17281 ttcgcactgg tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc
    17341 acatgcgttg ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca
    17401 acaataaaag tgacaccgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc
    17461 tgctgtacag taatcgttga ttgttgcaga tctgcgccat agccaataat cattgaagcg
    17521 ccgtccagta atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca
    17581 aaattttcgt ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca
    17641 ttttccagcg taccggcatg cacttcagcc cgaccaccca cattcacgga caggttttgc
    17701 gtcttaccgg aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc
    17761 tcaacaatag tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac
    17821 gtatcactgg ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg
    17881 gcttccccac ctggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt
    17941 gtcccattaa ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct
    18001 tccagtacac gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca
    18061 acgctgaatt caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt
    18121 gcgcctccct cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg
    18181 ccattgttac cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca
    18241 gcccccgtga ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca
    18301 gtaagttgcg tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc
    18361 atcaagtttt tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat
    18421 tgctacctaa gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa
    18481 acaactctgt ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc
    18541 ctgaataaaa atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc
    18601 aaacggtcga aatagcgcag aaaattacgt tttgcctctt gccaccttcc cactctgccg
    18661 ctaatattcg tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca
    18721 ccaccttgac atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc
    18781 gtagctcagt tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact
    18841 cggacgcacc agattttctt aatctggtct tctccttttt ccctctgttt cttctctgta
    18901 tccaatacgt taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat
    18961 ggcaactttg ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga
    19021 gcagcttaaa ccgcttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg
    19081 gacagaaagt gaaaagaacc acgaagccgg tgggatctat ctttttactg atgaaaaaag
    19141 cgcccttgcc tatctggaaa aacatactgt ccgacttaaa aatctcggcg ttgaggaagt
    19201 tgtcgccaaa gttttcgatg tcaatgagcc actgagtcaa atcaatcagg caaaactcgc
    19261 ctgacagaat ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa
    19321 attctaaaaa tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg
    19381 taagtaacaa tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta
    19441 ttctcgcttc tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag
    19501 gccggtgtcg ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt
    19561 gccagtattg aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa
    19621 gccagccatc tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta
    19681 ccgcgaattc tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca
    19741 cgacagcaaa aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt
    19801 acaaatgctg gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta
    19861 gcggtgattg ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt
    19921 cccagtccgt ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg
    19981 ggaacatcct tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc
    20041 attgaagatg ataaacaaca cgtcgttttt aaccgcgata acgcaagtga aaagctaaat
    20101 attacgttga tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc
    20161 tacgagccct tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggtgaa
    20221 gagggccttt tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca
    20281 gactggagcc aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg
    20341 tttgccgagc gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc
    20401 gaacgcaata aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat
    20461 gaagccgtac aggaaattgt tcctcatctg aatgaacacg accataaacg gttcagtaaa
    20521 ggccttgcca gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgc
    20581 ctacttgcct tacgtgaagc gggaatcatt catattctcg ccctcggtga agactacaaa
    20641 atggaaatta atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac
    20701 gtttttattg atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg
    20761 ctacgcgagc aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg
    20821 ttacagcaac ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg
    20881 cacgaccagc ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct
    20941 cgggcggtcg taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa
    21001 agctatcagc actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga
    21061 atattgtttt caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc
    21121 agtgcggcgc ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc
    21181 gttgaatttt caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg
    21241 tttgcgccaa agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca
    21301 cccaatgtac aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg
    21361 ctgcttatcc gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca
    21421 atgtgactgt taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc
    21481 gcggcatacc cagcggttgc gattcgggcg ttatcctcac aacagccgat gcgagcatga
    21541 tcgtcgctgc gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt
    21601 tgcatagagg aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca
    21661 tactcaacca gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata
    21721 tcggcataac taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg
    21781 atacgccatt ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca
    21841 tcgaccatgc ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatga
    21901 ccgaagataa aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat
    21961 cttacgccag gaaacacatc tcccacggct tgcggataga gacacagcag ccccgtcaat
    22021 agtaacaacg gcagcaatcc atacataaca ccaacatagg cgacctgctg taagggatta
    22081 aatttagact gggttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa
    22141 taaaatcgcg tttgttttgc cgctcgctcc agccacccct gacgacgaat gcgatagtgg
    22201 tgaccattac ccccaacggc attgatcagc acaaagccga gccagcatgc gagtaacaaa
    22261 aatccgcaaa cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccagcgca
    22321 aagtgattta tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc
    22381 cataatctga ccgctttgca gtaaagataa atcttctcac cataatcggt gactgatttc
    22441 ttcacgccac gagcacgaag caaggcatgc agccccagca ccaaccacat cccaaccagc
    22501 aatactccgg caatgatcaa ccacaccggc caaaattcgg gagcgaactg tggcacatag
    22561 ttcgctaact ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt
    22621 taatcaaatg ttgaccgaac cggcgataca gatgcggttt cccggcacca ggtagctgat
    22681 attgatagta tttattttgc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa
    22741 agatcaatgc gtgttccggg caggcgctga cgcaaatggg cggaaagcct tttgccagtc
    22801 gggactcagc gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa
    22861 cctggtacgg acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc
    22921 gcacgatccc ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag
    22981 catcttcgca gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat
    23041 cggtgactgg aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt
    23101 tacggcatgc gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg
    23161 cataccgaac ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa
    23221 aaaagattac tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat
    23281 cccctaatgc agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg
    23341 ggttctgaaa ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca
    23401 taaatcgctc gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg
    23461 catattcagg tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt
    23521 aatatggcgc ttgcatcagc atggcgactt tgtaaggacg aggatatgtc tggcactcaa
    23581 aacgaacggc gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg
    23641 ccgcgtagtt ttcactcatg gcctgcgttg agtaatagta atcatcctgg cttcccgtca
    23701 tacaagcgat atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagct
    23761 cctcttctgc aaccgaatag ggtggcgaga gaaacacggc gcgcgccacc agctgcccag
    23821 ccgcagaatg ctggcgaatg aactctgcca ggatttcagc cggttctggt tcggttaccg
    23881 tatcaacggg gtgacgcatc ccctgtaacc attgccgctt cgcctcgtca acttcgctga
    23941 ctttcgctaa cggcaattca tcccgatggt tcatctcacc cattccttac acaggcagta
    24001 gattgtgtgc tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc
    24061 gggtcggaca accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag
    24121 catgcatatc gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat
    24181 ccttgtcgaa tacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca
    24241 tcagttttac cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt
    24301 tttcctgagt catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat
    24361 cgccccggta attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca
    24421 gggtgacggc gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg
    24481 cgtcattgat tggcgtgtag tttttggttt catcgtaggc atcttcagaa ccaaaaagct
    24541 ctttcgccac ttcacgctgc agtttcaatg gcaaacccga accaataaaa ttgatatggg
    24601 tatgcgtcat gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg
    24661 cttcattggc atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc
    24721 cccagtactc ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt
    24781 gactcagctc acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt
    24841 cacccgcttc cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct
    24901 tgaacacccc tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc
    24961 ataggccgta gtcatcgaac aaattcagac ccaccaggtt accaatcaca cggccatcgt
    25021 ctttatcttc aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta
    25081 cacaagtgtt accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt
    25141 gggtcataca acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg
    25201 ccgccggacc taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc
    25261 ctggcggaat ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt
    25321 tacgtgctgt ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg
    25381 gcacgacatg gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct
    25441 tcatctcctg acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca
    25501 ggtttttcga acccataatt gcgccagttc ccgcaccgcc gctgtggtta ccgctattca
    25561 acatgccaga gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt
    25621 ccggactggt caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga
    25681 aatcggcttt ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg
    25741 ccttcccttc gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc
    25801 cacccatatg ggcatcgaca actaaatttc ctttggtaaa agtagaaagt gaggtgatat
    25861 ttacgcgaga actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt
    25921 tattcgcttc atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc
    25981 cgaagcccat gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc
    26041 ctgtcgtgag attgactctt aatatattac ctgtccaacc gttagccatg attttttcct
    26101 ttgcaagatt cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca
    26161 ggcgtttgcg cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac
    26221 ggtggccatc atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg
    26281 atcgacggta atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt
    26341 catgcattgc ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata
    26401 caggccgccg ccagagccaa ccccgttgtc gccaaagaaa taattgcggt ggattttgat
    26461 acgggagaag aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg
    26521 gtggcaaccc gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa
    26581 ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg
    26641 ggcaattgtt agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa
    26701 gccaatatct aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac
    26761 acagtgctga caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg
    26821 tccccaacac cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat
    26881 aaactaatcc tttatggtta ggagaaaaaa taatgtgatt at
//
LOCUS       DAZFVO020000058        26690 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFVO020000058 DAZFVO020000000
VERSION     DAZFVO020000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.058"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            86..1684
                     /gene="rtcR"
                     /locus_tag="ACHKNB_RS11280"
                     /old_locus_tag="pgaptmp_002256"
     CDS             86..1684
                     /gene="rtcR"
                     /locus_tag="ACHKNB_RS11280"
                     /old_locus_tag="pgaptmp_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HGU4195756.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1666..2424)
                     /locus_tag="ACHKNB_RS11285"
                     /old_locus_tag="pgaptmp_002257"
     CDS             complement(1666..2424)
                     /locus_tag="ACHKNB_RS11285"
                     /old_locus_tag="pgaptmp_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HGU4195757.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2441..3271)
                     /gene="glpG"
                     /locus_tag="ACHKNB_RS11290"
                     /old_locus_tag="pgaptmp_002258"
     CDS             complement(2441..3271)
                     /gene="glpG"
                     /locus_tag="ACHKNB_RS11290"
                     /old_locus_tag="pgaptmp_002258"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HGU4195758.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3316..3642)
                     /gene="glpE"
                     /locus_tag="ACHKNB_RS11295"
                     /old_locus_tag="pgaptmp_002259"
     CDS             complement(3316..3642)
                     /gene="glpE"
                     /locus_tag="ACHKNB_RS11295"
                     /old_locus_tag="pgaptmp_002259"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HGU4195759.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3832..5337
                     /gene="glpD"
                     /locus_tag="ACHKNB_RS11300"
                     /old_locus_tag="pgaptmp_002260"
     CDS             3832..5337
                     /gene="glpD"
                     /locus_tag="ACHKNB_RS11300"
                     /old_locus_tag="pgaptmp_002260"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HGU4195760.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5391..5996)
                     /locus_tag="ACHKNB_RS11305"
                     /old_locus_tag="pgaptmp_002261"
     CDS             complement(5391..5996)
                     /locus_tag="ACHKNB_RS11305"
                     /old_locus_tag="pgaptmp_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195761.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5999..6766)
                     /locus_tag="ACHKNB_RS11310"
                     /old_locus_tag="pgaptmp_002262"
     CDS             complement(5999..6766)
                     /locus_tag="ACHKNB_RS11310"
                     /old_locus_tag="pgaptmp_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195762.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(6769..7479)
                     /locus_tag="ACHKNB_RS11315"
                     /old_locus_tag="pgaptmp_002263"
     CDS             complement(6769..7479)
                     /locus_tag="ACHKNB_RS11315"
                     /old_locus_tag="pgaptmp_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195763.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7495..9003)
                     /locus_tag="ACHKNB_RS11320"
                     /old_locus_tag="pgaptmp_002264"
     CDS             complement(7495..9003)
                     /locus_tag="ACHKNB_RS11320"
                     /old_locus_tag="pgaptmp_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4195764.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9128..11575)
                     /gene="glgP"
                     /locus_tag="ACHKNB_RS11325"
                     /old_locus_tag="pgaptmp_002265"
     CDS             complement(9128..11575)
                     /gene="glgP"
                     /locus_tag="ACHKNB_RS11325"
                     /old_locus_tag="pgaptmp_002265"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HGU4195765.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11594..13027)
                     /gene="glgA"
                     /locus_tag="ACHKNB_RS11330"
                     /old_locus_tag="pgaptmp_002266"
     CDS             complement(11594..13027)
                     /gene="glgA"
                     /locus_tag="ACHKNB_RS11330"
                     /old_locus_tag="pgaptmp_002266"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGU4195766.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13027..14322)
                     /gene="glgC"
                     /locus_tag="ACHKNB_RS11335"
                     /old_locus_tag="pgaptmp_002267"
     CDS             complement(13027..14322)
                     /gene="glgC"
                     /locus_tag="ACHKNB_RS11335"
                     /old_locus_tag="pgaptmp_002267"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGU4195767.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14340..16313)
                     /gene="glgX"
                     /locus_tag="ACHKNB_RS11340"
                     /old_locus_tag="pgaptmp_002268"
     CDS             complement(14340..16313)
                     /gene="glgX"
                     /locus_tag="ACHKNB_RS11340"
                     /old_locus_tag="pgaptmp_002268"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HGU4195768.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16310..18496)
                     /gene="glgB"
                     /locus_tag="ACHKNB_RS11345"
                     /old_locus_tag="pgaptmp_002269"
     CDS             complement(16310..18496)
                     /gene="glgB"
                     /locus_tag="ACHKNB_RS11345"
                     /old_locus_tag="pgaptmp_002269"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HGU4195769.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18769..19872)
                     /gene="asd"
                     /locus_tag="ACHKNB_RS11350"
                     /old_locus_tag="pgaptmp_002270"
     CDS             complement(18769..19872)
                     /gene="asd"
                     /locus_tag="ACHKNB_RS11350"
                     /old_locus_tag="pgaptmp_002270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGU4195770.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20065..20658
                     /gene="yhgN"
                     /locus_tag="ACHKNB_RS11355"
                     /old_locus_tag="pgaptmp_002271"
     CDS             20065..20658
                     /gene="yhgN"
                     /locus_tag="ACHKNB_RS11355"
                     /old_locus_tag="pgaptmp_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HGU4195771.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20715..22055)
                     /gene="gntU"
                     /locus_tag="ACHKNB_RS11360"
                     /old_locus_tag="pgaptmp_002272"
     CDS             complement(20715..22055)
                     /gene="gntU"
                     /locus_tag="ACHKNB_RS11360"
                     /old_locus_tag="pgaptmp_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HGU4195772.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22059..22586)
                     /gene="gntK"
                     /locus_tag="ACHKNB_RS11365"
                     /old_locus_tag="pgaptmp_002273"
     CDS             complement(22059..22586)
                     /gene="gntK"
                     /locus_tag="ACHKNB_RS11365"
                     /old_locus_tag="pgaptmp_002273"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4195773.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLMVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22725..23720)
                     /gene="gntR"
                     /locus_tag="ACHKNB_RS11370"
                     /old_locus_tag="pgaptmp_002274"
     CDS             complement(22725..23720)
                     /gene="gntR"
                     /locus_tag="ACHKNB_RS11370"
                     /old_locus_tag="pgaptmp_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HGU4195774.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23944..24639)
                     /gene="yhhW"
                     /locus_tag="ACHKNB_RS11375"
                     /old_locus_tag="pgaptmp_002275"
     CDS             complement(23944..24639)
                     /gene="yhhW"
                     /locus_tag="ACHKNB_RS11375"
                     /old_locus_tag="pgaptmp_002275"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HGU4195775.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24762..25799)
                     /gene="yhhX"
                     /locus_tag="ACHKNB_RS11380"
                     /old_locus_tag="pgaptmp_002276"
     CDS             complement(24762..25799)
                     /gene="yhhX"
                     /locus_tag="ACHKNB_RS11380"
                     /old_locus_tag="pgaptmp_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HGU4195776.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26132..26620
                     /gene="yhhY"
                     /locus_tag="ACHKNB_RS11385"
                     /old_locus_tag="pgaptmp_002277"
     CDS             26132..26620
                     /gene="yhhY"
                     /locus_tag="ACHKNB_RS11385"
                     /old_locus_tag="pgaptmp_002277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4195777.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         6651 a         7140 c         6658 g         6241 t
ORIGIN      
        1 ttaaatcttt acgtccgtaa acggagattt tccacaaaac caattcaccg ataatgaaat
       61 atcgtctttt ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggtac
      121 cgtgctggat tatgccggac gcggcagtca gcgctggtcg aaatggcgtc cgacgctttg
      181 tttatgccag caagaatcgt tagtcatcga tcggctggaa ttgttgcacg acacccgctc
      241 gcgctcgcta ttcgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaacagaagt
      301 cgtgggcgtt gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct
      361 gcatgatttc gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat
      421 caccaccggc acccacgtcg cgcagatttg ctggtttctg ctggcggaag cgcgttacct
      481 gcccgcccgg ctgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcgcagg
      541 tgaagtgacg attatcgatc tcgatttaag ccgttataac gccatcgcca gccgctttgc
      601 cgaggaacgc cagcaaacgc ttgattttct taagtccggc attgccacgc gtaaccccca
      661 cttcaaccgc atgattgagc agatcgaaaa agtggcgatc aaatcccgcg cgccgattct
      721 gcttaacggt ccaaccggcg cgggcaagtc atttctggcg cgacgcatct tcgagttaaa
      781 acaggcgcgg catcagttta gcggcgcgtt tgtggaagtg aactgcgcca ccctgcgcgg
      841 cgataccgcc atgtcgacgc tgtttggtca tgtaaaaggc gcgtttaccg gggcgcggga
      901 atctcgtgaa ggtttattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg
      961 cgaactgggc gcagacgaac aagcaatgct gctaaaagcc attgaagaga aaacctttta
     1021 cccgtttggc agcgatcgcc aggtgagcag tgattttcag cttatcgccg gaacggtgcg
     1081 cgatttgcgc cagctggttg ccgaaggcaa atttcgcgaa gatctgtacg cgcggatcaa
     1141 tctctggacc ttcaccctgc cgggtctacg ccagcgccag gaagatattg aaccgaacct
     1201 tgattatgaa gtggagcgcc acgccacact cactggcgac agcgtgcgtt ttaacaccga
     1261 agcgcggcgc gcctggttgg cttttgcgac ctctccccag gcaacatggc gcggtaactt
     1321 tcgcgaactt tctgccagcg tcacgcggat ggccaccttt gccactagcg gacgcatcac
     1381 tctggacgtg gttgaagatg agataaaccg tctgcgctat aactggcagg agagccgccc
     1441 ctccgcgctt acggcgttgc tgggcgcaga ggcagaaaac atcgaccttt ttgacaggct
     1501 gcaactggaa cacgtgatag ctctctgtcg ccaggcaaag tcgctttccg ctgccggacg
     1561 cctgcttttt gatgtttcgc gccagggcaa agccagcgtc aatgacgcgg atcggctacg
     1621 caaatacctg gcgcgttttg gtctgacgtg ggaagccgtg caggatcagc acagctccag
     1681 ttgaatatga tggtccgtca gcacctgcat tacgcttact ggcggcgggg cgtcggtgta
     1741 gacggcatct accatgctga tgctgcccat attgaccatc gcgttacggc caaatttcga
     1801 gtgatcgaca accagcataa cgtggcgcga gttctcaata atggcgcgct tggtgcgaac
     1861 ttcgtggtaa tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat
     1921 gccgaaatca aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc
     1981 atcgcggctg cgtaattcgc caccggcgag aataatgcgg aaatcttctt ttaccatcaa
     2041 cgtgttagca acattgagat tgttggtgac aatacgcaaa ttgctgtgat taagcagcgc
     2101 gtgcgccacc gcttccggcg tggtgccgat atcgataaac agtgtcgagc cgttggggat
     2161 ttgctccgcc actttgcggg caatcctctc tttttcttcg gtctgggtgg ctttgcgatc
     2221 gtgccacggc gtgttaaccg aactggaagg cagcgccgca ccgccatggt gacgcaggat
     2281 caggttttgc tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa
     2341 atgctctacc agctcttcag tactgacata accctgctgt ttaaccagtt cgataatacc
     2401 gttgtgacgt tgtgtttgtt tcatttataa atccctggaa ttattttcgt tttcgcgcat
     2461 tgagcgaatc aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt
     2521 tcgccatcga catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga
     2581 taattaaccc acgttgcagg taaatgccac tttgcggatc gcgttcacca cgtagccaga
     2641 cgtagcccat cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt
     2701 tttgctgcac atagccgctt aacagggcgc taatgagagt aataacaatt agcttaccgc
     2761 taccgaggcg tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga
     2821 tatgcatcag cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca
     2881 gtgttggatc gaatggccag gccagccata acatcacttc ctgatcgccg agaatttgca
     2941 tggcgataaa caccaccacg caggcgatca tcaccaccca ggttaccgga cctgcgcgtt
     3001 cacgcaaggc ggcaaagaaa ggataacggc gataatgcag gccactgccg gtatggcctg
     3061 cctgccagct ggccgccaga taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct
     3121 ccgcccgtac gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt
     3181 gttgaatcgt gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc
     3241 gggggttagc aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg
     3301 ggacagtata aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gttgccaggc
     3361 ttcaaagccg ccgtcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc
     3421 cgcgcctttg ctgctattgc cgtgataaca catcaccatc accggagtgt caaagtcgtt
     3481 atcacgcata aacgcgccca gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc
     3541 catagcgaaa ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt
     3601 ctggtgcgcg tcggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac
     3661 aaacaagtgg gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg
     3721 ttatacatat cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat
     3781 atgttcgata acgaacattt atgagcttta acgaaagtga atgagggcag catggaaacc
     3841 aaagatctga ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct
     3901 ggacgcggtt tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc
     3961 gccagttcaa aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg
     4021 gtcagcgagg cgctggctga acgtgaagtg ctgctgaaaa tggctccgca tatcgccttc
     4081 ccgatgcgtt ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcggatt
     4141 ggtctgttta tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aaccggtttg
     4201 cgttttggcg caaactcagt gttaaaaccg gaaattaagc gcggattcga atattctgac
     4261 tgttgggtag acgacgcccg tctggtactc gccaacgccc agatggtggt gcgtaaaggc
     4321 ggcgaagtgc ttactcggac tcgcgccacc tctgctcgcc gcgaaaacgg cctgtggatt
     4381 gtggaagcgg aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt
     4441 aacgccaccg gcccgtgggt gaaacagttc ttcgacgagg ggatgcatct gccttcgcct
     4501 tatggcattc gcctgatcaa aggcagccat attgtggtgc cgcgtgtgca tacccagaag
     4561 caggcctaca ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac
     4621 gaattttcca tcatcggcac caccgacgta gagtataaag gcgatccgaa agcggtgaag
     4681 attgaagaga gtgaaatcaa ctatctgctg aaagtgtata acgcgcactt taaaaaacag
     4741 cttagccgtg acgatatcgt ctggacctac tccggtgtgc gtccgctgtg tgatgatgag
     4801 tccgactcgc cgcaggctat tacccgtgat tacacccttg atatccatga tgaaaacggc
     4861 aaagcaccgc tgctgtcggt attcggcggt aagttgacca cctaccgcaa actggcggaa
     4921 catgcgctgg aaaaactgac gccgtattat cagggcattg gcccggcgtg gacgaaagag
     4981 agcgtgctgc cgggtggtgc cattgaaggc gaccgcgacg attatgccgc tcgcctgcgc
     5041 cgccgctatc cgttcctgac tgaatcgctg gcgcgtcatt acgctcgcac ttacggcagc
     5101 aacagcgagc tgctgctcgg caatgcggga acggtaagcg atctcgggga agatttcggt
     5161 catgagttct acgaagcgga gctgaaatac ctggtggatc acgaatgggt ccgccgcgcc
     5221 gacgacgccc tgtggcgtcg cacaaaacaa ggcatgtggc taaatgcgga tcaacaatct
     5281 cgtgtgagtc agtggctggt ggagtatacg cagcagaagt tgtcgctggc gtcgtaagga
     5341 tgatgcccgg ttgtgaagtg caaccgggct atttggattc cttagactat tcattgtaaa
     5401 taattgaata acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc
     5461 cacttcttcc tctggtttct attttttctg caccaatggc aataatatcc caatgcaaaa
     5521 ccaagctgtg cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa
     5581 aaattcaggg gaagccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct
     5641 gatttctctg gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc
     5701 gaaaagacca gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat
     5761 tgccaaccga tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt
     5821 tgtagttcct gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc
     5881 agaaaagtta acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat
     5941 ggcacgataa caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt
     6001 attttcctgg tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc
     6061 agtcgtctac ccagacctga tgctgtgtgc cctgttctgg ctgctttggt gggttctcaa
     6121 tcgcttgccg cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt
     6181 gggtggtaat ggcgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc
     6241 tgttcaataa acaggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat
     6301 ccagcaatgg cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt
     6361 cgacagcttc atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa
     6421 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
     6481 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
     6541 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
     6601 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
     6661 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
     6721 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
     6781 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
     6841 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
     6901 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
     6961 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
     7021 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
     7081 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
     7141 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
     7201 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
     7261 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
     7321 accgacgaac ttttcccctt catccagggc ctggttaata agttgctgaa caatgccttc
     7381 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     7441 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     7501 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     7561 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     7621 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     7681 gttaatcgct agcagtttta tatttttatc gtcgttcatt tgtgttgcgt aataacggaa
     7741 ggtgtaacct attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc
     7801 catcatactt gccacttctg tttcctgcgg cgagatcatg cggacatttt tcatgacctg
     7861 ggattgcatc accgtctggc taccagagtc ttccgggcgt tgccaggtct ggatctcctg
     7921 atcgttaccg ccaaccgtgc gccagttggt aattgcacca ctgaagatgt cacgcacttg
     7981 ttgttcggtc agggaattaa ccgggttatc tacattgacg atgaaaacaa acgcttcacg
     8041 ggcaaaaggc gtgtaaatca aagtgacgcc cgattcctcc gcgcgttttt tctgcccacc
     8101 ggaaggttga gccacaaaga taatatcggc attaccttta acaatcttgt tatatgcttc
     8161 gggagtacga gagttcgcca gatattccca ttcgtgaaaa tcttccggca aaacgcttag
     8221 tgcataaaag gcagaggcat aaatggggta cgccgccgtt gcgccatcaa gtcgcggcca
     8281 gttttgcgtg aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt
     8341 atcgggtcgc caggcccagg tatcaatatt ttcacgcaca ccgacgccgg gattaagctg
     8401 tttatataaa taagcctggt aaccggcgac aaaaccaagc atgagaagga taaacaagag
     8461 agaattacgg caacgtaacc cggacgtgcc cgttaaagga tatctggtcg gccagccgta
     8521 ccccagcgaa aaactgatat gcgcgccaac aggtataatg actaaccacc agtactcacc
     8581 accgactaat gaggctaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc
     8641 cgctcccatc tggcgaaatt gataatctgc cagatgcagc gcgattatcc agacccccag
     8701 ggttaataat gcgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc
     8761 tggtgcacta tcgtccgccc acactcgccc cgagaaaaat agcattaatg cgcccagcgc
     8821 cagcataaaa atgttgagca gaaggtaaat tgttggcgtg ataaagatat tacaaacttc
     8881 aataataccg gcaagtccga cacccagcat ggcaataacc gccgccaaaa actgcgccag
     8941 aatggggatg ccgaaaaaag tcattaccag cgaggtcaaa atccattttc tgttttgcat
     9001 tattctttcc attctttctg aatggcgaaa ttatactccc cgagtcccct tgccccttct
     9061 ggacactttt ccgaaatgat ggcgaaaaaa acgggaccct ttggccccgt tctatttatt
     9121 agtgaaatta caatctcacc ggatcaatat gccagatatg atcggcgtac tctttgatag
     9181 tacggtcaga agagaagtag cccatattgg caatgttcag catcgctttt gcggtccact
     9241 cttcctgaag ctcgtagagt tcgtcgactt tatcctgaca atcgacatag ctgcgataat
     9301 ccgccagaac ctgatagtga tcgccgaagt tgatcagcga atcaaccaga tcgcgatagc
     9361 gacccggatc ttccggactg aatacaccgc tgccgatttg cgtcagcacc tgatgcagct
     9421 cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt tcttccactt
     9481 cttccgcagt gttaccaaag ataaagatat tgtcagcacc gacatgatcc agcatctcga
     9541 cattcgcacc gtccaacgta ccgatagtaa gcgcaccgtt aagcgcaaac ttcatgttac
     9601 tggtgccgga agcttccgtc cccgccagcg aaatctgttc agacagatcg gccgccggaa
     9661 tgatcaactg cgccaggcta acgctgtagt tcgggatgaa cacgactttc agcttgtcgc
     9721 caatctgcgg atcgttgttg atcactttcg ctacgtcatt gatcaaatga ataatgtgct
     9781 tcgccatgta ataggccgaa gccgccttac cgccaaaaat attcacgcgc ggtacccact
     9841 tcgcatccgg gtcggccttg atgcggttat agcgggtaat cacatgcaac acattcatca
     9901 attgacgttt gtattcgtga atacgtttga tttgtacatc gaacaacgcc tttggattca
     9961 ccaccacatt cagctgctgg gcgatatact ctgccagacg ctttttgttc tccagcttcg
    10021 cctgatgcac agcgtgatta accattggga aatcacagtg ttgttgcagc tcattaagca
    10081 ggctaaggtc ggtgcgccag ttacggccca ggtgttcgtc cagcacggct gaaagcgatg
    10141 ggttcgctac cgctagccag cgacgcggcg tcacaccgtt ggtgacgttg gtgaaacgac
    10201 ccgggaagat tttcgcaaag tcggcaaaca acgattgcac catcagatta gagtgcagtt
    10261 ccgatacacc gttaactttg tggctcacaa caaccgccag ccaggccata cgcacacgac
    10321 gaccgttgga ttcatcaatg atcgacgccc gtcccagcag atcggtatcg ttcggatact
    10381 gttcctgcaa ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc aggtgacgcg
    10441 gcagaatttt acccagcata tcaaccggcc aggtttccag cgcctcgctc atcagcgtgt
    10501 ggttagtgta ggagaagacc tgacaacaca cctcaaacgc gtcgtcccag ctaaattggt
    10561 gctcatcgat cagcagacgc atcatctcag gaatcgacag taccggatgg gtatcattga
    10621 gatgaatcgc gattttatcc gctaggttat cgtaggtttt atgcaactga taatggcggc
    10681 ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc ctgacgcagg cgcagctcac
    10741 gcccggagta ggtggagtca tccggataca gtacgcgaga tacgttctcg gagtggtttt
    10801 tatcttccac tgccgcgaag tagtcaccct ggttgaattt accgaggtta atttcgctac
    10861 tggcttgcgc actccacaaa cgcaacgtgt tggtcgcgtc ggtgtcgtaa ccagggatta
    10921 tctgatcgta agcgactccc agaatctctt cggtttcaat ccaacgcgtt tttttacctt
    10981 cctgctgaat gcgaccacca aaacggactt tatagcgcgt gttgtggcgt ttgaattccc
    11041 acgggttacc gtattccagc cagtagtctg gcgactcttt ctggctaccg ttaacgatgt
    11101 tctgtttgaa cataccgtag tcatagcgga tgccgtaacc gcgccccggc aaccctaacg
    11161 tcgccagaga atcaaggaag caagccgcca gacgtcccag gccaccgtta ccgaggcctg
    11221 ggtcattttc ttcatcaatc agctcttcga gatttaaccc catcgcttcc agtgcgccct
    11281 gtacatcttc gtaaattcct agcgacaaca tggcgttgga gagcgtacgg ccaatcaaaa
    11341 actccatcga caggtagtaa acctgacgag tttcttgcga caactgggca cggtttgaac
    11401 gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc agccattcat
    11461 gtttattggc gacgaccggg tcctttccaa tcgtaaacat cagcttgtaa gcgatagagt
    11521 gcttaagagc ttctacgcta agcgtgggcg atgaatatgt aaacggagca ttcatatagg
    11581 cgtttcctga aaactatttc aagcgatagt aaagctcacg gtacgacttc gccgcgacct
    11641 gccagctaaa atccattgcc atagcctgac gttgcacaaa ccgccacagt gaaggacggg
    11701 accacagtac aaaagcacgt cgaatagccc gtaacagcga ccaggcatta ctatcttcaa
    11761 agacaaaccc actggcgacg ccatctgcaa ggttctcaag agaacagtca gaaaccgtat
    11821 cagcaagccc accggtgcgc cgcactaacg gcagcgtacc gtacttcaat ccataaagtt
    11881 gcgttaagcc gcacggttca aaacggctgg gcaccagaat gacgtccgcg ccgcccataa
    11941 tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc cacctgaccg gggtattccg
    12001 ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt agcgccagct
    12061 gcccgccctg ctccagaaga cccggtaagg cttccagcac caggtcgaga cctttctggc
    12121 tggtcagacg gctcaccact gcaaaaagcg gcactttatc gtcaacctta agccccattg
    12181 cgatttgtaa ctggcgctta ttttccgctt tatcttccaa cgtatcgcgg gtgtaacgcg
    12241 aggccaacag taagtccgtc tctggactcc agattttctc gtccacgccg ttcagtacgc
    12301 cggaaagacg cccttcacgg tgacgctgtt gcaacagacc ttccataccg taggcaaact
    12361 gcggttcggt gatctcgcga gcgtaggttg gactgaccgc cgtaatgtga tcggcatagt
    12421 acagaccggc cttcaggaaa gagatttgtc cgttgaattc cagcccatga atattaaaga
    12481 atgaccatgg caattggatg tcattcatat gatgtgcata aaacatgcct tgataggcca
    12541 ggttgtgcac agtaaacacc gacttcgccg gacgcccgcg cgccgccaga tacgcaggcg
    12601 caaggcctgc atgccagtcg tgcgcatgca ccacatcagg acgccagaat gggtcaagcc
    12661 cgctggccat ttctgcccca acccacccca gcagcgcaaa acgcaatacg ttgtcggtat
    12721 aggcaaataa gttggtatcg tgatacgggc ttcccggacg atcatagaga tgcggcgcgt
    12781 caatcaggta tatgccaacc ccgttgtaat gaccgaacaa cagcgtgata tgtccggcga
    12841 aggtatcacg acgggatact acctgcgcat cggtaacgcc acggcgaata tcgggaaatg
    12901 caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt aatgccccaa
    12961 taacatcagc cagaccgccg gttttaagca gcgggaacat ctctgaacat acatgtaaaa
    13021 cctgcattat cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg ttaccagcac
    13081 gatgccttct tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt caccaatcac
    13141 catgccttcc ggaataacac aagcacgatc gatgacgcag cggcgcagac ggcacgagcg
    13201 acctacccat acttccggta acaatacggc ggaatcaatg ttgcagaatg aattcacgcg
    13261 aacgcgcgag aacagaacgg actgcaccac caccgaaccg gagatcacac agccgccgga
    13321 taccagtgag ttaagggtca tcccgtggct accggagcga tcctgcacga atttcgctgg
    13381 cggtaatgat tcattgtagg tgcgaattgg ccaattgcga tcgtacatat ccagttccgg
    13441 caccacagag gccagatcga ggttcgcttt ccagtaagct tccagcgtac ccacatcgcg
    13501 ccagtacggc tcggcatccg ggtcggattg tacgcaagag agcgggaacg ggtgcgcata
    13561 ggccagaccg gcttcggtga tcttgggaat caaatctttg ccaaagtcgt ggctggagtt
    13621 ctcatcacga tcgtcttctt ccagcagttc atacagatag tcggcgtcaa agacgtagat
    13681 acccatactc gccagagatt tgctcggatc gttcggcatt gacggtgggt tagcaggttt
    13741 ttcaacgaat tcgataattt tatcgttctc atcaaccgcc ataacgccaa atgcggaggc
    13801 ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt gcgccttttt cgacgtgatc
    13861 gataagcata cgcgagtagt cttgcttgta gatatggtcg cccgccagga tcaccacgta
    13921 ttccgcttta taacggcgga taatgtcgag gttttgggtg accgcatctg cggtgccgcg
    13981 ataccagttt tcccctttca ttctctgctg tgctggcagc agatcgacaa actcgttcat
    14041 ttcttcattg aagaatgacc agccgcgctg aatgtgctgc accagagtgt gggactggta
    14101 ctgggtgatc acgcccatac gacggatccc ggagttgatg cagttagaca gcgcaaagtc
    14161 gataatgcgg aacttaccgc cgaagtgtac ggccggtttt gctcgcttat tggttaaatc
    14221 cttcaggcgg gtaccacgtc ctcccgccag tatcagggca acagatttca atggcagctg
    14281 gcgcgccaac attaagtgat cgttcttctc taaactaacc atgactaact ccttttttat
    14341 catctctgga acacacacaa tccgtgtgca ggtccctgcc agacagccgt aatcactggg
    14401 ttatcctctc cagcgaatgg gggaatggcg tgccactccc cagcaggtaa aacaatctct
    14461 gttacctcaa gcgtggcgtt aattgcgatc aaaaagcgat ccgagagcag aatttgcagc
    14521 tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg atttagccaa
    14581 cggacattgc cgtcgccttc ttcccaccag cgattctcca ccaaagcggg aatgcgcttg
    14641 cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg cgaccagtcc
    14701 aaccaggtta attggttatc ctgacagtag gcattgttat tgccatgctg gctgtgacca
    14761 tgttcgtcac cggccagtaa catcggcgta ccctgggaga gcaacaacgt tgttaacagg
    14821 gcgtgaatgc tgtcgcgccg ccgttcaacc aggtcaagag aaccgcctaa cccttcttta
    14881 ccatgattgt tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc gtttgcttca
    14941 ttgtgtttat ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg cgcggtgacg
    15001 agattaatcg cggcactcgg cagacgacca ttacgtttaa aaacatcgct ggaggcagca
    15061 aaacgcccgg caaacgcccc cagaggcaaa tcataatgta gccagaaacg acgggcagca
    15121 tcgcggaaat gatcgttcca ctcggcaaac agcggcggga aatttcccac ctgataacca
    15181 ccaggagcga tatcccacgg ttcagcaatt aacttcacct gcgagagcac cgggcagttc
    15241 tggatagcgg taaacaacgg cgcatcctga cggaactctg gcgtacggcc catgactgcc
    15301 gccagatcaa agcggaaacc atcgacgtgg caggtttcta cccaataacg caggcaggcg
    15361 ctggcataat ccaccaccgc cggatgactc aaattgagcg tgttgccgca accggtccag
    15421 ttgtgataat cgccgtcttc tcttatccaa taatagctac ggttatcgat cccacgcagc
    15481 gagaataacg ggccgtcgag gtccagttcc gcactatggt tgagcacgat atcaagaatg
    15541 acttcgatac ccgctttatg cagtgctttg attgcatcgc gaaactcatc cagcgccgtt
    15601 tctggcgagc aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt gtaaccccag
    15661 tagttactta gccccatgcg ttgcagacgt ggttcactgg caaactgcgc cactggcagc
    15721 agttccagcg cggtaatgcc caattgtttc aaatagttga tcatcaccgg atgcccgagg
    15781 gctttataag tgccacggat ctcgaccggg atctccgggt gcaagtacgt taatcctttg
    15841 acatgggctt cataaatgat ggtgctgccc cacggcgtgc gcggcggggc atcatcttcc
    15901 cagtcatagt gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt gtcgcgatag
    15961 tcaggttcat tatgaccggc gtgcagcagc gggttatctt taaactcccc ctcaatttgc
    16021 cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc gatgcccctc ggcgggttgc
    16081 caggggccat gaacgcgata accataacgc aaacccgggc gcgcatccgg cagataaccg
    16141 tgccaaatgt cgccactgtg ccctggcaag tcatagcgat gttcctggcc attggcgtca
    16201 aagacacaca gttctacccg ctcggcatga gcggagaaaa gtgtgaagtt gacgccctga
    16261 ccgtcgtaat gcgcgccgag gggagcgggt ttgccaatgg cgagttgtgt cattctgcct
    16321 cccgaaccag ccagatagtg gccagcggtg gtagcgtcag gcttagtgaa tgctgacgac
    16381 cgtggctggc aatctcatcg ctgtgtaccg tgccgccatt gcctgcatta ctgccgtgat
    16441 agtgcatgga atcggtattg aggatttcac gccatttgcc cggctggttt atgccgaagc
    16501 gataatcatg acgcggcacc ggcgtaaagt tactggcaac gatgatttcg ttaccctctt
    16561 tatcgcgacg cacaaagatc agcaccgagc gttctttgtc atccaccacc agccattcaa
    16621 agccgtacgg gtcaaaatcc agttcatgca ttgctttatg gtggcggtag gtgaggttca
    16681 gatcgcgcac cagacgctgg acaccgtggt gccagttatc gccgccttcc aacagatgcc
    16741 agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc aaattcgtta cccatgaaca
    16801 gtagtttctt gcccgggaat gcccacatcc agccatagta ggcgcgcagg ttcgcgaatt
    16861 tctgccatgc gtcgcccggc atgcggtcga gaatcgattt tttaccgtgg accacttcat
    16921 catgcgacaa cggcaggacg aagttttcag tgtagttgta gagaatcccg aaggtcagtt
    16981 tatcgtgatg atactgacga taaaccgggt cgagcttcat gtagtccagg gtgtcatgca
    17041 tccagccgag gttccacttg taccagaagc ccagaccgcc catatcctgc ggacgagaaa
    17101 cgccagggaa atcggtagac tcctcagcca ttgtcaccgc accggaaacc tgctcaccaa
    17161 gaatacggtt ggtattacgc aagaattcaa tcgcttcaag attctcgcgc ccgccaaatt
    17221 cgttcgggat ccactccccc tctttacggc tgtagtcgcg ataaatcatt gacgccaccg
    17281 catcgacgcg cagcgcatca ataccaaaac gttcaatcca gtaaagcgcg ttaccgacga
    17341 ggaagttact gacttcacgg cgaccatagt tgtagatcag cgtgttccag tcctgatgat
    17401 agccttcacg cggatcgctg tgttcataca agttcgtgcc atcaaattcg gcaagcgcaa
    17461 agtcatcagt cgggaagtgg cctggcaccc agtcgagaat cacgttcaga ccagctgcgt
    17521 gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg gttggtgcat
    17581 acaggccggt tggctgataa ccccaactgc catcgaaggg atgctcgtta atgggcagta
    17641 gttcgaggtg ggtaaagccc atccatttag cataaggcac cagttgatcg gccagctcgc
    17701 ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca ggaacccagg tgaacttcat
    17761 aaatagagat tggcgcatca aactgattcg cttttttgcg ctcttcagtc tgtacaacct
    17821 tttccggcag cccgcaaata agagacgcgg tttccgggcg catttgcgct tcgaaggcat
    17881 aagggtcgga cttcagacgc aagttgccat tggcatcaat catctcgtat ttatagagct
    17941 gaccgttatg cgccccaggg ataaacagtt cccagatgcc gctctcttta cgcaggcgca
    18001 tcgggtgacg gcgaccgtcc cagtagttga attgcccaac caccgagacc cgacgggcgt
    18061 ttggagccca gacagagaaa cgcgtacctg tgacgccatc catagtatct gcatgcgcgc
    18121 ctaaggtttc atacgggcgc aggtgagtac cttcagataa tagccaggca tccatttcct
    18181 ggattagcgg accaaaacgg taaggatcat caatcaggtt ttgctgacca tgccagacaa
    18241 cagccaactg atagcggaaa aaattcttac gtcgcggaat gacgccgcta aagaatcccc
    18301 gtgagtcgag acactccagt tttgcgagtt tgcgcccggt tttcggttca atcacccaca
    18361 catcggtagc gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt ttatgcattc
    18421 ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc acgtctctat
    18481 cgatacgatc ggacatgctt gtcttcctgt tttattgtgt caccccatcc aattggattt
    18541 ttgacttctg gttgtgacat ttttttgacc tgaacggcgc agcactctgt gcatcctctc
    18601 tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg tattcagcca cccttaaaga
    18661 atagccaatg ctctatttaa ctcccggtaa atcatgaaac atctgcgctt actcctgtat
    18721 tacgcactaa caggggcggc atcgcgcccc agatttaatg aataaagatt acgccagttg
    18781 acgaagcatc cgacgcagcg gctccgcggc cccccacagc agctggtcgc ccacggtaaa
    18841 ggctgacagg aactctggtc ccatattcag cttacgcagg cggcctaccg gcgtggtcag
    18901 cgtgccggta acggcagctg gggttagctc acgcatagtg atttcccgat cgttcggaac
    18961 gactttcgcc cacggattgt gcgcagccag cagttcttcc acggtcggaa tagacacatc
    19021 ttttttcaat ttaatagtga atgcctggct gtggcagcgc aatgccccga cacgcacaca
    19081 taaaccatct accggaatta cggaagatgt gttgaggatc ttgttggttt ccgcctgccc
    19141 tttccactct tcgcggctct gaccgttatc gagctgtttg tcgatccacg gaatcaggct
    19201 acccgccagc ggcacgccaa agttatccac cggcagctca ccgctacggg ttaaggttgt
    19261 gactttgcgt tcgatatcga gaatagcaga ggacggggtc gcgagttcat ctgccacatg
    19321 gccatacaga tggcccatct gggttaataa ctcacgcata tgtcgcgcac caccgccgga
    19381 agcggcctgg taggttgcaa cggacaccca atcaacaaga tcattggcga ataaaccacc
    19441 caacgacatc aacatcaggc ttacggtaca gttaccgcca acaaaagtcc tgatgccatt
    19501 atttaatccg tcggtaatga cgtcctgatt gacggggtca agaatgatga tggcgtcatc
    19561 tttcatgcgc agagacgatg ctgcgtcaat ccagtaacct tgccatccgc tttcacgaag
    19621 ctttggatag atttcgttgg tataatcgcc gccctgacag gtcacaatga tatcgagggc
    19681 ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca gtggttccgc caaaagacgg
    19741 cgcagcctgg ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt cgaagtcgcg
    19801 ctcttcaacc atgcgttgca tgagaacgga gccgaccata ccgcgccagc cgataaaacc
    19861 aacatttttc ataagcgttt ttttcctgca aagatgtgtg ctgtataaat gtgccggtct
    19921 cctcttggca catctttcac catacaaaaa gcagccaaag tcgcaagtga aattaatcaa
    19981 tgatagcgaa gccatcagta atgcgactta tcctgctttg ttagcacgca gaaagtccgc
    20041 ggcaattatc agggaatttg agttatgaat gaaatcattt ctgcagcagt tttattgatc
    20101 ctgattatgg atccgctcgg aaacctacct attttcatgt ccgtactgaa acatactgaa
    20161 ccgaaaagac ggcgggcaat catggtgcga gagttgctta ttgctctcct ggtgatgctg
    20221 gtgttcctgt ttgcgggtga gaaaattctg gcatttctta gcctacgagc agaaaccgtc
    20281 tccatttctg gcggcatcat tctgtttctg atcgccatta aaatgatttt ccccagcgct
    20341 tcaggaaata gcagcgggct tccggcaggt gaagagccat ttatcgtgcc gttggcaatt
    20401 ccgttagtcg ccgggccgac tattctcgcc acgctgatgt tgttgtctca tcagtacccg
    20461 aatcagatgg ggcatctggt gattgctctg ctgctggcct ggggcggcac ctttgtcatc
    20521 ctgctacagt cttcgctatt tttacgtctg ctgggcgaga aaggggtgaa cgcacttgaa
    20581 cgcctgatgg gattgattct ggtgatgatg gcaacccaga tgttcctcga cggcattaga
    20641 atgtggatga aggggtaacg aggagagttt ttcgaaaaga acccggtagc gtcacaacta
    20701 ccgggcgaac aaacttaact caacagctgg aacgcaatca tcccaacgat ggcaccgaca
    20761 gtgccgagga tggtttccat catggtccag gttttcagcg tttcggcttc ggtcgcgccg
    20821 gtaaatttac cgaacaacca gaaaccggcg tcgttaacgt ggctgacaac aatcgaacca
    20881 ccagcgatac aaatcgacag cgccgccatt tgcgcaccgg agtagttcag ttgttcaata
    20941 accggcatca ccagtcctac cgccgttaaa caggctacgg tggcagaacc ctgaatgatg
    21001 cgcactgcag ctgccagcac gaagcaggtg atggctatcg gcaggcccat gccggttaac
    21061 gcttcgccca gtgccggacc tacgccagag tcaaccagca cctgtttgaa cacgccgcct
    21121 gcaccaatca ccagcagaat gatccccgcc ggttgcagcg cgtgaccgca aatctccatc
    21181 actttgtctt ttggcatgcc ctgacgcatt gccaggccat aaatcgctac cagacaagca
    21241 accagaatcg cggtaaacgg atgaccaata aactcgaacc attcgtaagc ggtagagcct
    21301 tctggcacaa aacgcgcggc aatggttttc agccccacca gcaccagcgg caacaggatc
    21361 agcgacaggc tgaatccgaa agatggcatt ttgccttcgc cgagatgcgg ttcgctgatg
    21421 tcgtcaggaa tatgcaactc cacgtagcgg ctgatgaaat taccccacag cggcccggca
    21481 ataatcattc ccggaattgc cgcacacagg ccaatcagga tcatccagcc aaagtcggca
    21541 ttcatctgcg atgccagcag catcggcgct ggtccaggca ccagaaatgc cgccgctgcc
    21601 gccacgcctg caaataatgg gattaccagc ttcaccaggt tcgtaccggt gtggcgcgcc
    21661 attgagaaag caacgctaat cagcagaact atcgccactt caaagaacag cggtagcgca
    21721 cagaccagcc ccgcaaggcc gatggcataa tgcgcgcggc tgtgaccgaa ggatttgagc
    21781 attttgacgg caatctgatc gactgcgccg gtttcatgta agatcttgcc aaacatagct
    21841 cccagggcga caaccaccgc caggaagccg agggtgcctc ccatcccttt ttccatcgtc
    21901 gctgcgattt tatcgagcgg cataccggaa aaaaggccag cccccatgga caccaccatc
    21961 aaagccagga aagcgtgcat acgcgccttc atgactaaaa acagcagcag taaaacagac
    22021 cctactgctg ttaaaacaag cgttaatgta gtcactactt atttgccttt tttaataacc
    22081 tcaatggtgc ttgccacaac accttccagc ggttgatcga tatccaccat cagtacatcg
    22141 gtttcgtccg cacccggctc ctgcagcgtt tcaaactgcg tcaccaacat ttgggtttta
    22201 aagaaatggc ctttgcgcgc tttcaggcgg ctttcaatca catcaaaatc gcctttcagg
    22261 tagatgaaag agagattcgg attaccttca cgcagcaagt cgcgatagtg ttttttcaat
    22321 gcagaacaga cgatcagtga cactttatta gtgcgctgca tagcaaacgc ggcgtcgttc
    22381 agcgcctgca accacggttt gcgatcgtcg tcattcagcg gttcgccaga cgccattttt
    22441 tcgatattgc gccgtggatg gaggaaatcg ccatcaagaa acgcggcatg aagttgatgc
    22501 gccacttcac tggcgaccgc agatttgccg ctgcctgaga cgcccatcaa gacgtaaatg
    22561 tggtgatcat ggttagtcgt gctcaaagtg gtgcccccac aatacaagaa ttaaaaatgt
    22621 tacgggtaac tgttatcggt aacattgtcc ggccggacaa tatcagaagc aatatccatc
    22681 cgtgccttaa gtgtataagt gtgagctact tcaaatttgt gggcttaaat agatccgccc
    22741 ggtgacaagg tgaaacctaa atctaacatt ttcggtgtca cagattcgcc acgaatacgc
    22801 gccagcaggc gttcagcgcc aatactgccc atccgctcac gcggcgtcag cacgctcgcc
    22861 agtcgtggct ccatcacctg accaatgtca tgaccgtgga aaccggcaat cgccatatcg
    22921 tcaggaactt ttaaccccag acgctgacat tcaaacgccg cgccgaccgc cagatcatca
    22981 ttagtacaga acacgccatc cagctgcgga tattcccgcc tcgcctggcg aatcagttca
    23041 ataccggaag agtaagaaga agattgctca accatcacgc tatatggcac caggtctgca
    23101 tccagcatcg cctgttcgta tcccttctgt ttgatgatag tacgttcgtc gagacgtgcg
    23161 ccgagataag caatgtggcg atgcccgcga gcaataatgg cggtggtcat ctggcgtgcc
    23221 gcttcaaagt tatcaaaacc gacggcaata tcgaggcacg gcgacttgct gtccatcaac
    23281 tccaccaccg ggatccccgc cacttcaatc atctttaagg tgcgtggcgt gtgagtacgt
    23341 tcggtgagga tcagtccatc gatattccat gagagcatgg attcgaggcg ttcttgctcc
    23401 atttccggtt tataaccgta gtgcgccagc atggtttgat aaccgtgcgc gtcggtgacg
    23461 ctttcgattc cgcgtaatac ttccgcgaaa acctggttgg tgagagaagg taacaggacg
    23521 ccaatcgccc ggctggtggc gttagagagg atatcgggcg cacgattggg aatataaccc
    23581 agttcatcaa gagcggcggc aatcttgccg cgtagagcga cggaaacctg ctccgggtta
    23641 cgcaaaaagc ggctgaccgt cattttggtc acgcctacac ggtcagccac atcctgaagt
    23701 acgggtcttt tctttttcat cgtcctgaag gtacaaaaga gatagattct cttagtttaa
    23761 cacggacgca tcagaacctt cccggggaaa acagtgcttg tttacaatta ttagatatac
    23821 actaaatcaa ttgcatcgca ttgtgctaac gacgcggcaa aaccccggaa gcgtgcatca
    23881 gcacgcgacc ggagcgagcg aggaagcctg caaaaaggca ggcttcaaaa atgccgttga
    23941 gttttaaacc ggcggcagat cgaacagtaa cacttcgctg tcgctatccg catggatgga
    24001 gattgcctgc tcatcccaga ttgccagacc atcgctggtc gaggctttca cgccgttaat
    24061 ggtgacattg cctttcacca cctggatcca gacgcggcgt tcagcggcaa tctgatgcac
    24121 cgactgctca tctttcagca acgcccaacg atacagttcc atatcctgat gcactttcag
    24181 cgaaccatct cgcgcatccg gcgagagcac cagctgtttg ccctgcacgg catcgaagcg
    24241 acgctgttca taacgcggcg taatgccatt ttcttcaggc atgatccaga tctgatacag
    24301 atgcagacgc tcggtgctgc ttgggttgta ctctgagtga cgaatacccg tacccgcact
    24361 cataatctgg aactcacccg ccggaacctg ctctttattg cccatgctgt cctgatgctc
    24421 aacagtacct tccagcacgt aggtcaaaat ttccatatct ttatgcgggt gagtgccgaa
    24481 gccctgccct gcttcaatca cgtcgtcgtt aatcacgcgc agcgcggaga agcccataaa
    24541 gttcggatcg taatagttgg caaaagagaa agtatgccag gagtccagcc agccatgatt
    24601 tgcatgacca cgttcatttg ctttgcgtaa gtagatcatt tgtattcgcc ccctgaatga
    24661 tttcgatggg cttagtgtgg acccgatctg cctgggatga tagagggtga aaactgaccc
    24721 ctctgttcaa aaaaattgaa ctattcgagg ggccatcaaa cttacttagc gagagttact
    24781 gtggagggag aggcttgctc aaatccgcgt tcaaggattt ccagattggt aagaacttca
    24841 gattccttga cgtaatttgg cgcaccgtgg gtgatggttt gatacaacgc atcataaacg
    24901 cgcccgtaat cgcccatctc cggcttcatc tcttctctga ccgtcacgcc ctcgtcattg
    24961 acatactcca gcacaccgac cgaattatcc gctgcgaatc ccggttcgcc cggcataata
    25021 ttagccttca ggctggtttc ctgctggtcg ataccgtatt taataaacga acctttctta
    25081 ccgtgaacga taaatttcgg ataatcgatt ttcaccagat ggctggtttt gacgatggct
    25141 ttcaggtcgc cataaaacag ttgcgcttca aaggtgtcgt caggattggc tttattacgc
    25201 aggctgcgga tgtcataagc gacgtgatcc gggcgaccga acagagaaat aatctggtcc
    25261 atcgtatgca caccaaggcc ataaaacgcg ccatcctgcg gcagcccagg tttggtttct
    25321 gccaccgggc ggtaatagtc aaaatggctt tccacttcaa caatctctcc caacttgcca
    25381 ctttcaatcg cttttttcgc tgtcaggaag caggagtcaa agcgacgatt ctgatacggc
    25441 gtgacggtca gccctttgct tttcgccaac gcaaacagct ctttcgcctg cgcaagtgtc
    25501 ggagtgaacg gtttttcgac cagcacattt ttcccggctt ccagcgcgcg cttcgcgtac
    25561 tcgaagtggc tgtccgcgtg ggtgcagaca acaaccagct taacatcggg atcgtttagt
    25621 acttcgtcga gatcgctggt gaaatggata tgggaataaa tgggagcctg ttcttccggc
    25681 ttcgcatggc gacgaaaaat atgcgcgaca tgccaactat ccttgcggtt aagtacatac
    25741 ggcagatggt aacgggtggt gcttttgccg aagccaataa aggcgcagtt gatgaccatg
    25801 atttcgtcct ttttaaggtg gttatttaca ccttagcgca aagcggacgt ggttcctact
    25861 ggagtgcgca taacgaacac aagcactccc gtggataaat tgagaacgaa agatcaaaaa
    25921 aaaagccagc acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt
    25981 caggttctcc gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact
    26041 tgtccaacac tttttgcaaa aaaatgcatt tgactcgcat ttgaaagtca atgatgttga
    26101 aagggacatt taccccaaag aggacaaagg aatgagtgag atagtaatac gccacgcaga
    26161 aacacgggat tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac
    26221 actacaggtg cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcccgg
    26281 catcaagcaa ctcgtcgcct gtattgatgg agacgtcgtg ggccatctca ccattgacgt
    26341 gcaacagcgc ccacgccgca gtcatgttgc cgattttggt atctgtgtcg actctcgttg
    26401 gaagaaccgc ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg
    26461 gttgcgggta gatcgcattg aactaaccgt gtttgtcgat aacgccccgg caattaaggt
    26521 ctataaaaaa tacggctttg aaattgaagg gactggtaag aagtacgcat tgcgtaatgg
    26581 tgaatatgtc gatgcatatt atatggcgcg ggtgaagtaa gatagtgccc tttttctgag
    26641 atggaaaaag ggtgtcattc aaaatcgaca taccttcctt taaggtattt
//
LOCUS       DAZFVO020000059        26085 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFVO020000059 DAZFVO020000000
VERSION     DAZFVO020000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.059"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..1787)
                     /locus_tag="ACHKNB_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /pseudo
     CDS             complement(1..1787)
                     /locus_tag="ACHKNB_RS11390"
                     /old_locus_tag="pgaptmp_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2311..4239
                     /gene="thrS"
                     /locus_tag="ACHKNB_RS11395"
                     /old_locus_tag="pgaptmp_002279"
     CDS             2311..4239
                     /gene="thrS"
                     /locus_tag="ACHKNB_RS11395"
                     /old_locus_tag="pgaptmp_002279"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGU4195778.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4243..4785
                     /gene="infC"
                     /locus_tag="ACHKNB_RS11400"
                     /old_locus_tag="pgaptmp_002280"
     CDS             4243..4785
                     /gene="infC"
                     /locus_tag="ACHKNB_RS11400"
                     /old_locus_tag="pgaptmp_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGU4195779.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4882..5079
                     /gene="rpmI"
                     /locus_tag="ACHKNB_RS11405"
                     /old_locus_tag="pgaptmp_002281"
     CDS             4882..5079
                     /gene="rpmI"
                     /locus_tag="ACHKNB_RS11405"
                     /old_locus_tag="pgaptmp_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGU4195780.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5132..5488
                     /gene="rplT"
                     /locus_tag="ACHKNB_RS11410"
                     /old_locus_tag="pgaptmp_002282"
     CDS             5132..5488
                     /gene="rplT"
                     /locus_tag="ACHKNB_RS11410"
                     /old_locus_tag="pgaptmp_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGU4195781.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5605..5729
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5611..5655
                     /gene="pheM"
                     /locus_tag="ACHKNB_RS11415"
                     /old_locus_tag="pgaptmp_002283"
     CDS             5611..5655
                     /gene="pheM"
                     /locus_tag="ACHKNB_RS11415"
                     /old_locus_tag="pgaptmp_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HGU4195782.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5939..6922
                     /gene="pheS"
                     /locus_tag="ACHKNB_RS11420"
                     /old_locus_tag="pgaptmp_002284"
     CDS             5939..6922
                     /gene="pheS"
                     /locus_tag="ACHKNB_RS11420"
                     /old_locus_tag="pgaptmp_002284"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGU4195783.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6937..9324
                     /gene="pheT"
                     /locus_tag="ACHKNB_RS11425"
                     /old_locus_tag="pgaptmp_002285"
     CDS             6937..9324
                     /gene="pheT"
                     /locus_tag="ACHKNB_RS11425"
                     /old_locus_tag="pgaptmp_002285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGU4195784.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVTVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9329..9628
                     /gene="ihfA"
                     /locus_tag="ACHKNB_RS11430"
                     /old_locus_tag="pgaptmp_002286"
     CDS             9329..9628
                     /gene="ihfA"
                     /locus_tag="ACHKNB_RS11430"
                     /old_locus_tag="pgaptmp_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HGU4195785.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9729..10709
                     /gene="btuC"
                     /locus_tag="ACHKNB_RS11435"
                     /old_locus_tag="pgaptmp_002287"
     CDS             9729..10709
                     /gene="btuC"
                     /locus_tag="ACHKNB_RS11435"
                     /old_locus_tag="pgaptmp_002287"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HGU4195786.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10772..11323
                     /gene="btuE"
                     /locus_tag="ACHKNB_RS11440"
                     /old_locus_tag="pgaptmp_002288"
     CDS             10772..11323
                     /gene="btuE"
                     /locus_tag="ACHKNB_RS11440"
                     /old_locus_tag="pgaptmp_002288"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HGU4195787.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11323..12072
                     /gene="btuD"
                     /locus_tag="ACHKNB_RS11445"
                     /old_locus_tag="pgaptmp_002289"
     CDS             11323..12072
                     /gene="btuD"
                     /locus_tag="ACHKNB_RS11445"
                     /old_locus_tag="pgaptmp_002289"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HGU4195788.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12150..12614
                     /gene="nlpC"
                     /locus_tag="ACHKNB_RS11450"
                     /old_locus_tag="pgaptmp_002290"
     CDS             12150..12614
                     /gene="nlpC"
                     /locus_tag="ACHKNB_RS11450"
                     /old_locus_tag="pgaptmp_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4195789.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12861..13574
                     /gene="ydiV"
                     /locus_tag="ACHKNB_RS11455"
                     /old_locus_tag="pgaptmp_002291"
     CDS             12861..13574
                     /gene="ydiV"
                     /locus_tag="ACHKNB_RS11455"
                     /old_locus_tag="pgaptmp_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HGU4195790.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13637..15073
                     /gene="selO"
                     /locus_tag="ACHKNB_RS11460"
                     /old_locus_tag="pgaptmp_002292"
     CDS             13637..15073
                     /gene="selO"
                     /locus_tag="ACHKNB_RS11460"
                     /old_locus_tag="pgaptmp_002292"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HGU4195791.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15077..15268)
                     /gene="hemP"
                     /locus_tag="ACHKNB_RS11465"
                     /old_locus_tag="pgaptmp_002293"
     CDS             complement(15077..15268)
                     /gene="hemP"
                     /locus_tag="ACHKNB_RS11465"
                     /old_locus_tag="pgaptmp_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HGU4195792.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15400..16446)
                     /gene="aroH"
                     /locus_tag="ACHKNB_RS11470"
                     /old_locus_tag="pgaptmp_002294"
     CDS             complement(15400..16446)
                     /gene="aroH"
                     /locus_tag="ACHKNB_RS11470"
                     /old_locus_tag="pgaptmp_002294"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HGU4195793.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16603..17436)
                     /gene="ppsR"
                     /locus_tag="ACHKNB_RS11475"
                     /old_locus_tag="pgaptmp_002295"
     CDS             complement(16603..17436)
                     /gene="ppsR"
                     /locus_tag="ACHKNB_RS11475"
                     /old_locus_tag="pgaptmp_002295"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HGU4195794.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17769..20147
                     /gene="ppsA"
                     /locus_tag="ACHKNB_RS11480"
                     /old_locus_tag="pgaptmp_002296"
     CDS             17769..20147
                     /gene="ppsA"
                     /locus_tag="ACHKNB_RS11480"
                     /old_locus_tag="pgaptmp_002296"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HGU4195795.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(20204..21850)
                     /gene="fadK"
                     /locus_tag="ACHKNB_RS11485"
                     /old_locus_tag="pgaptmp_002297"
     CDS             complement(20204..21850)
                     /gene="fadK"
                     /locus_tag="ACHKNB_RS11485"
                     /old_locus_tag="pgaptmp_002297"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HGU4195796.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYNYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(21907..22200)
                     /gene="ydiT"
                     /locus_tag="ACHKNB_RS11490"
                     /old_locus_tag="pgaptmp_002298"
     CDS             complement(21907..22200)
                     /gene="ydiT"
                     /locus_tag="ACHKNB_RS11490"
                     /old_locus_tag="pgaptmp_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4195797.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(22197..23486)
                     /gene="ydiS"
                     /locus_tag="ACHKNB_RS11495"
                     /old_locus_tag="pgaptmp_002299"
     CDS             complement(22197..23486)
                     /gene="ydiS"
                     /locus_tag="ACHKNB_RS11495"
                     /old_locus_tag="pgaptmp_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4195798.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(23542..24480)
                     /gene="ydiR"
                     /locus_tag="ACHKNB_RS11500"
                     /old_locus_tag="pgaptmp_002300"
     CDS             complement(23542..24480)
                     /gene="ydiR"
                     /locus_tag="ACHKNB_RS11500"
                     /old_locus_tag="pgaptmp_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HGU4195799.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(24500..25264)
                     /gene="ydiQ"
                     /locus_tag="ACHKNB_RS11505"
                     /old_locus_tag="pgaptmp_002301"
     CDS             complement(24500..25264)
                     /gene="ydiQ"
                     /locus_tag="ACHKNB_RS11505"
                     /old_locus_tag="pgaptmp_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HGU4195800.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            25580..>26085
                     /locus_tag="ACHKNB_RS11510"
                     /old_locus_tag="pgaptmp_002302"
     CDS             25580..>26085
                     /locus_tag="ACHKNB_RS11510"
                     /old_locus_tag="pgaptmp_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGU4195801.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNA"
BASE COUNT         6519 a         6208 c         6778 g         6580 t
ORIGIN      
        1 gaacagctga catgttttca tgatttttag cggccaacag tgtgaataat tgacgttgac
       61 taaaagaata tttttttgca aaagtactca gtgttgatat ataagataac acgacgtctt
      121 tattaccctt gctcatggcg atgtataaag caggggttcc atgtgctgtc gcgcctttta
      181 ataagtccat gatgttgatt ttgctgagtt tatatttaac ggcgatccca taaactttag
      241 agaggaaccg tgtgacacac aaagggtgat tattttccat tgcagcaaat aaccctggtt
      301 caccatcacg tgttttcatg gctaaaatat ccatcaccat tttttttgtt taaaagttct
      361 tgtgctattg gagtggttaa tgcattcaga aagatatcga ttatttcatc gaatccatac
      421 aacatggaga tatagagtcc aggtgtttcg ttagcacttt tagtttgcag taatttaacc
      481 agattatctt catgaatgat atgattgtat accagtgact ggatttcttg cataaatatt
      541 tttacgttgt cagtttgtcc gttcatcata gccagaaata aagcaggtgt accaacgcta
      601 ttctgcgcag ttattatttc aatgatttct ttttcaggga gtatgggcaa ctgtttgaat
      661 cgtgtaataa gctattgagt ctggcctgtg gccattatta acaacattgc cgatggatgt
      721 ataggggcaa atggcattcg tgtcgtgata cccgctaaaa tagcaactgg aactccctca
      781 ggtaatagag gaagaatagc aattgcacag ttgcgaacat acctatcagc ccaggtctga
      841 gcatcgggag ctgaactgat gaacttatca agcgagagaa agcgccgtgc tgtcctgtgg
      901 gtgcctctga cggcaacgtt ggtatcgttc gggtcgtaaa cagacacaac ataatttaat
      961 tgtccataca ctgactgctt tattcgtagc cttactgaca aagtatgatt gtctatatat
     1021 aaaatcgcgg acatatgact gacattgttt gccatcattc gacaaaagaa aatatctaaa
     1081 gcgaagccaa aatccttact cgcgataacg cgactccctg caggaggatg gcgaagtaat
     1141 gctctgaact ccgcatcccg gactaagggg atatgctgct gtatggattc aacggaggag
     1201 taatgtttaa tcaggttgac tttgcctttt ccatttctca atgaatcaag ggcgtattgc
     1261 aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcaaaaaaca tgcactacca
     1321 ttgagattta ctgttgggtg tatagttttt tctctatgga tttttttaga cagtttcata
     1381 caatggttac gatcttcagc agttaactgt tcctgttcaa tataatccag tatgcttcgc
     1441 aaatggttag ctttaatagg cgtggtttcc agtaataagg aaatgaaatc ttttttatag
     1501 tgtaatgtaa tgagtatatt ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa
     1561 agggcagtgg aataacaaat ttgtgatatt tttcgacagt ttgcggtaaa ttcctgaata
     1621 acacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat gagctggcgc
     1681 gcaaatttat taataggctc tgatgaaggt gttggtatgt cgctatcgat gtgcaatcta
     1741 tgaggagact tataatgagt tctgataatg atatcgtttt gactcataat ctattcgcct
     1801 tatatatatc tggaggtttt gaggatgatg attaattatt atcgccagaa cgaaatgtgc
     1861 acgaaaaatt gttataagaa ttgtgtcact tttatcattg tgatatgacc acttcgtcat
     1921 taatctcatg agcgtttaaa atactaccgc caaatcacgc aacgaacttt gctggattca
     1981 gtttatgctg ctgtaaatcc gctcgcagta aacctttcag acgcaccgtg atgttatcag
     2041 tggttcttta agcgttttgc tggtgtactc actacaaacg aattgcgaat cgaatcaatg
     2101 tgaaacggaa aggtacaatc tccctttttt tagtgttaac atcgctcaac cgggtggaag
     2161 gtgagaggtt aatgtctttc atttgtgtac ctgattgcga accaatttag catttgttgg
     2221 ctagatggtt tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt gatctttcgt
     2281 gtgggtcacc actgcaaata aggatataaa atgcctgtta taactcttcc tgatggcagc
     2341 caacgccatt acgaccacgc tgtaagcccc atggatgttg cgctggacat tggtccaggt
     2401 ctggcgaaag cctgtatcgc agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg
     2461 attgaaaacg acgcacaact gtccatcatt accgccaaag acgaagacgg tctggagatc
     2521 attcgtcact cctgtgcgca cctgttaggg cacgcgatta aacaactttg gccgcatacc
     2581 aaaatggcaa tcggcccggt tattgacaac ggtttttatt acgacgttga tcttgaccgc
     2641 acgttaaccc aggaagatgt cgaagcactc gagaagcgga tgcatgagct tgctgagaaa
     2701 aactacgacg tcattaagaa gaaagtcagc tggcacgaag cgcgtgaaac attcgccaac
     2761 cgtggggaga gctacaaagt ctccattctt gacgaaaaca tcgcccatga tgacaagcca
     2821 ggtctgtact tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt accgaacatg
     2881 cgtttctgcc atcatttcaa actaatgaaa acggcagggg cttactggcg tggcgacagc
     2941 aacaacaaaa tgttgcaacg tatttacggt acggcgtggg cagacaaaaa agcgcttaac
     3001 gcttacctgc agcgcctgga agaagccgcg aaacgcgacc accgtaaaat cggtaaacag
     3061 ctcgacctgt accatatgca ggaagaagcg ccgggtatgg tattctggca caacgacggc
     3121 tggaccatct tccgtgaact ggaagtgttt gttcgttcta aactgaaaga gtaccagtat
     3181 caggaagtta aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac cggtcactgg
     3241 gacaactaca aagatgcaat gttcaccacg tcttctgaga accgtgaata ctgcattaag
     3301 ccgatgaact gcccgggtca cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat
     3361 ctgccgctgc gtatggccga gtttggtagc tgccaccgta atgagccgtc aggttcgctg
     3421 catggcctga tgcgcgtgcg tggatttacc caggatgacg cgcatatctt ctgtactgaa
     3481 gaacaaattc gcgatgaagt taacggatgt atccgtttag tctatgatat gtacagcact
     3541 tttggcttcg agaagatcgt cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc
     3601 gacgaaatgt gggatcgtgc tgaggcggac ctggcggttg cgctggaaga aaacaacatc
     3661 ccgtttgaat atcaactggg tgaaggcgct ttctacggtc cgaaaattga atttaccctg
     3721 tatgactgcc tcgatcgtgc atggcagtgc ggtacagtac agctggactt ctctttgcct
     3781 tctcgtctga gcgcttctta tgtaggcgaa gacaatgaac gtaaagtacc ggtaatgatt
     3841 caccgcgcaa ttctggggtc gatggaacgt ttcatcggta tcctgaccga agaattcgct
     3901 ggtttcttcc cgacctggct tgcgccagtt caggttgtta tcatgaatat taccgattca
     3961 cagtctgaat acgttaacga attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa
     4021 gcagacttga gaaatgagaa gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc
     4081 ccatatatgc tggtctgtgg tgataaagag gtggaatcag gcaaagttgc cgttcgcacc
     4141 cgccgtggta aagacctggg aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa
     4201 gagattcgca gccgcagtct taaacaattg gaggaataag gtattaaagg cggaaaacga
     4261 gttcaaacgg cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta
     4321 acaggtctgg aaggcgagca gcttggtatt gtgagtctga gagaagctct ggagaaagca
     4381 gaagaagccg gagtagactt agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt
     4441 ataatggatt acggcaaatt cctctatgaa aagagcaagt cttctaagga acagaagaaa
     4501 aagcaaaaag ttatccaggt taaggaaatt aaattccgtc ctggtacaga tgaaggcgac
     4561 tatcaggtaa aactccgcag cctgattcgc tttctcgaag agggtgataa agccaaaatc
     4621 acgctgcgtt tccgcggtcg tgagatggcg caccagcaaa tcggtatgga agtgcttaat
     4681 cgcgtgaaag acgatttgca agaactggca gtggtcgaat ccttcccaac gaagatcgaa
     4741 ggccgccaga tgatcatggt gctcgctcct aagaagaaac agtaaggcct tcaagtagca
     4801 ctgtctgtgg agccttcggg cttcacaggc tttgttcgcc tttgtttcgt ttattaacaa
     4861 tgcgaagtgg aagttattaa aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc
     4921 ttcaaaaaaa ccggtaaagg tggttttaag cacaagcacg ctaacctgcg tcacattctg
     4981 accaaaaaag cgaccaaacg taaacgtcac ctgcgtccaa aagccatggt ttccaaaggc
     5041 gatctgggcc tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt ttttaacttt
     5101 ttaattagaa tatagataca ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc
     5161 acgcgcacgt cacaagaaaa ttttgaaaca agctaaaggc tactacggtg cgcgttctcg
     5221 cgtataccgc gttgccttcc aggctgttat caaagctggt cagtatgctt accgtgaccg
     5281 tcgtcaacgt aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg cagcagcacg
     5341 tcagaacggt atttcttaca gcaaattcat caatggcctg aaaaaagcct ctgttgaaat
     5401 cgaccgtaag atcctggctg atatcgcagt attcgacaaa gtagcgttca ccgctctggt
     5461 tgaaaaagcg aaagcagctc tggcataagc cagttgaaag agggagctag tctccctctt
     5521 ttcgtttcaa cgccatcaaa acattgactt ttatcgccgt agccttttca ataaaggtct
     5581 tttgaagagt accaaaaggt aacgcaagca atgaatgctg ctattttccg cttctttttt
     5641 tactttagca cctgaatcca ggaggctagc gcgtgagaag agaaacggaa aacagcgcct
     5701 gaaagcctcc cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa aagcctgtct
     5761 catcaggtgt aattgtcgca gccagtctgg acacggccag cgcggagaaa ccggagcgta
     5821 tttaagtacg tgagaatttc gagcactgcc cgggaccaaa atggcaagta aaatagcctg
     5881 atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga ggaaaaccat
     5941 gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg cgtcagatgt
     6001 tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact taacccttca
     6061 gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg cggttatcaa
     6121 cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc
     6181 tgcactgaat gcgcgtctgg cggcggaaac gattgatgtc tccctgccag gtcgtcgcat
     6241 tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa gtttcttcgg
     6301 tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc ataacttcga
     6361 tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt tctggtttga
     6421 cactacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca tgaaagccca
     6481 gcagccaccg attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact acgaccagac
     6541 tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca tcagctttac
     6601 caacctgaaa ggcactctgc acgatttcct gcgtaacttc tttgaggaag atttgcagat
     6661 tcgcttccgt ccttcctact tcccgttcac cgagccttct gcagaagttg acgtcatggg
     6721 taaaaacggt aaatggctgg aagtgctggg ttgcgggatg gtgcatccga acgtgctgcg
     6781 taacgttggc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg ggatggagcg
     6841 tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa acgatctgcg
     6901 tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga actgtggtta
     6961 cgcgaatggg tgaacccggc gattgatagc gatgcgctgg cgaatcaaat cactatggcg
     7021 ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt ggtcgttggt
     7081 gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac aaaagtgaat
     7141 gttggcggcg atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg tcagggcctg
     7201 cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat taaagcggcg
     7261 aaactgcgtg gtgaaccgtc tgaagggatg ctgtgctcct tttctgagct gggaatttct
     7321 gacgatcata gcggcattat cgagctgcct gcggatgcgc cgattggcac cgacatccgt
     7381 gaatacctga aacttgatga caacaccatc gaaatcagcg tgacgccaaa ccgtgccgac
     7441 tgtttaggta tcattggtgt tgcgcgtgac gttaccgtgc tgaatcagct gccgctggtt
     7501 gaaccggaaa tcgttccggt tggtgcgacc atcgacgaca cgctgccgat tacagtcgaa
     7561 gcgccggaag cctgcccgcg ttatcttggc cgtgtggtaa aaggcattaa cgttaaagca
     7621 ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca
     7681 gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca cgctttcgat
     7741 aaagatcgca ttgaaggcgg cattgtggtg cggatggcga aagagggcga aacgttggta
     7801 ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc cgaccacaac
     7861 aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa tgacgaaacg
     7921 caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg tcgtgctcgt
     7981 cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga tccggcactg
     8041 cagcacaaag cgatggaacg tgcgacccgt ctactgatcg acatctgcgg tggtgaggct
     8101 ggtccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac catcacttta
     8161 cgtcgtagca aactggatcg cctgatcggc catcatattg cggatgagca ggtaactgac
     8221 attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgagtggca ggcagttgcg
     8281 ccgagctggc gtttcgatat ggagattgaa gaagatctgg tcgaagaagt cgcgcgtgtt
     8341 tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat gggtactcac
     8401 cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa aggctatcag
     8461 gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca tccaggcgtt
     8521 gaagccttac tgctgccaag cccgatctct gttgaaatgt cagcaatgcg tctttctctg
     8581 tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa ccgtgtgcgc
     8641 attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg cattcgtcag
     8701 gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg gaacctggca
     8761 aaagagaccg ttgatttcta tgatttgaaa ggcgatcttg aatccgttct cgacctgacc
     8821 ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca tccggggcaa
     8881 tccgcagcga tttatctgaa aggtgaacgt attggttttg ttggggttgt tcatcctgaa
     8941 ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga gtggaacaag
     9001 ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt
     9061 gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc cgaatgtaag
     9121 aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg cggtaagggt
     9181 gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac cagccgtaca
     9241 ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga
     9301 ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata
     9361 tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg aactgttttt
     9421 cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg gttttggtaa
     9481 cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat
     9541 tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa aaagccgggt
     9601 cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg ctctgcggcc
     9661 ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac ttcagcagaa
     9721 cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc tggttattat
     9781 gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt gaacaatgga
     9841 tctcgccagg tgactggttt tctcctcgtg gcgaactgtt cgtctggcag attcgcctgc
     9901 cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc gcagtaatgc
     9961 aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct aacggcgcag
    10021 gcgtagggct tatcgccgcg gtattgcttg ggcaagggca actcccaaac tgggcactag
    10081 ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt ttcgcccgtc
    10141 gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt atctgtagcg
    10201 cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag ctgatgtact
    10261 ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg ctggcattga
    10321 tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta gcacttggcg
    10381 agatctcggc gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg ctggtggcag
    10441 cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc tttattggtc
    10501 tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta ttacttcccg
    10561 gctgcgcgct ggcaggggcg agcgcattgc tgctggccga tgttgtagcg cgcttggcat
    10621 tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta
    10681 tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga ttaaaagcta
    10741 agatttttac tgaccacacc caggagaaac gatgcaagat tccattctga cgaccgtagt
    10801 gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg tgctgttgat
    10861 tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg agaatattca
    10921 gaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc agtttctgga
    10981 acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat ggggggtgac
    11041 gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga cgccatccgc tgtatcaaaa
    11101 attgattgcc gcagcgccga ccgcagtcgc gccggaagag agcggattct atgcccgtat
    11161 ggtcagcaaa ggtcgtgcac cgctgtaccc tgatgatatt ttatggaatt ttgagaaatt
    11221 tttagttggc agggacggat tggtcatcca gcgtttttcc ccggatatga cgccggaaga
    11281 ccccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat tgtgatgcag
    11341 ttacaggatg ttgcggaatc tacccgcctg gggccacttt ctggcgaggt tcgggctggg
    11401 gagatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttgct tgcacgaatg
    11461 gccggaatga ccagcggtaa gggaagcatt cagttcgcgg ggcaacccct ggaagcatgg
    11521 tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac gccgccgttt
    11581 gcaatgccgg tctggcacta cctgacactg catcagcacg ataaaacgcg taccgaacta
    11641 ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggtcgtag caccaatcaa
    11701 ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgctgca aatcacacca
    11761 caagccaatc ccgcaggtca attgctgctt cttgatgagc cgatgaacag tcttgatgtt
    11821 gcgcaacaaa gtgcgttaga caaaattctg agcgctctgt gtcagcaagg actggcgatt
    11881 gtgatgagca gtcacgatct caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta
    11941 aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc gccaaatctg
    12001 gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag aatgctgatt
    12061 tcgaccatct gatttcatta gtatcttgaa aatcgggtaa ttacgacgct aaattaatgc
    12121 aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg atcacagcac
    12181 tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga ctttctgatt
    12241 cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc acgccgtatc
    12301 gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt gtaacgatgc
    12361 gcgatcgttt cgatttgcag ctgccccgag aaaccaaaga acaagcctct atcggcacgc
    12421 aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg ggttccggac
    12481 aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac gcctctacca
    12541 gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat ttctggcagg
    12601 cgagacgaat ttagtatgtc gaaaaatgga cggcgatgat acatcgccgt ccatttactc
    12661 ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg tttataatca
    12721 ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca tggaacaaat gaaagttact
    12781 ccttatgtaa ttgttatgtc ttatggcctc atccctttta gccggatact gaaaaacatc
    12841 cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat tctgattgct
    12901 atttcctgcc gataagagat aaccagcaag accttgtcgg cgttgagtta attacccatt
    12961 tctccagtga agatggtacg gttcgtatcc cgacgagtcg ggttatagcg caacttaccg
    13021 aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca tgccaacatt
    13081 tttttattca acataaactc tttgcctggc ttaatttaac gccacaagtg gctacattat
    13141 tattagagag ggataattac gcaggcgaac tattaaaata tccgtttatt gaactgttga
    13201 ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa tcgcgatctt ttatccttat
    13261 cgcaaatgta tccccttgtg ttaggcaatt tgggagcagg taatagcaca atgaaagcgg
    13321 tttttgatgg tcttttcacc cgcgtcatgc tggataaaag ttttattcag caacaaatca
    13381 cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc gcaaatttcc ccttgttgca
    13441 attgcatcat cgcggggggc attgatacga cagaaatatt ggcacagatt acccccttcg
    13501 atttccatgc tctccaggga tgcctgtggc ctgccgttcc tataaatcag ataacgacgt
    13561 tggttcagcg ataaccctcc tgtttgctgg tgtttaagac gagagtaacc gtctacacta
    13621 tcaaacagga ggatctatga ccctgtcttt tattacccgc tggcgcgatg aattgccaga
    13681 aacctataca gcactttccc ctacgccttt aaataatgcc cggctgattt ggcataatac
    13741 cgaactcgct aacacgctga gtattccatc gtcgctgttt aaaaatgctg caggtgtctg
    13801 gggcggcgaa accctactgc ctggtatgtc accactggcg caggtttaca gtggtcatca
    13861 gttcggcgtc tgggcgggcc aactgggtga tgggcgcggc attttacttg gcgaacaact
    13921 gcttgctgat ggcactacaa tggactggca tctgaaaggt gctggcctga cgccttattc
    13981 gcgaatgggt gatggacggg cggttttacg ttcgacgata cgagaaagtc tcgccagtga
    14041 ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta agtatcgtca ccagcgattc
    14101 gccagtgtat cgggaaacgg tagagccagg tgcgatgctg atgcgtgtgg caccaagtca
    14161 tctgcgcttt ggtcatttcg aacattttta ctatcgccgc gagccggaaa aggttcgtca
    14221 gttagctgac tttgccattc gtcattactg gtcacatctt gaagatgatg aggacaaata
    14281 ccgtctctgg tttaacgatg ttgtcgcacg taccgcatcg ttaattgccc aatggcagac
    14341 ggtcggcttt gctcatgggg tgatgaatac cgacaacatg tcgctgctgg ggctgacgct
    14401 tgattacggg ccgtttggtt ttcttgatga ttacgaaccc ggttttattt gtaatcactc
    14461 ggatcatcaa gggcgttaca gctttgataa tcaacctgcc gtcgcgttgt ggaatttaca
    14521 gcgtctggcg cagacattgt caccatttgt tgccgtagat gccctgaatg aggccctgga
    14581 cagctatcag caggttttgt tgacgcatta tggacaacgg atgcggcaga aactgggctt
    14641 catgacggag caaaaagagg ataacgcgct actgaatgaa ttattcagtc tgatggcgcg
    14701 agagcgcagc gattataccc gcacattccg catgctgagt ctgaccgagc agcacagcgc
    14761 ggcgtcaccg ctacgtgatg agtttattga tcgtgcggca tttgatgact ggtttgcccg
    14821 ttatcggagg cgtttgcaac aagacgaggt tagcgatatt gagcgtcagc aactgatgca
    14881 aagcgttaac ccagctctgg tgttgcgcaa ctggttggcg caacgggcga ttgaagcggc
    14941 agaaaagggt gatatgacgg aattgcaccg cctgcatgag gcgttgcgaa atcctttcag
    15001 cgacagagct gatgactatg tcagtcgtcc acctgactgg ggtaaacggc tggaagtcag
    15061 ctgctcgagt taaaggctac ttggtcaaca gcagcttgcc agcctgagtt ttacggagca
    15121 gatattcttg cccgtcatga tcgataatca gtttgccatc cggacctaac agtgtctggc
    15181 tggtaattcg ccggatgggc tgcggggaag cggtcttgtg attggcatcc gtttcaggcg
    15241 tgagttttct gctatccgta taacgcataa aaattaataa taattgatat aacaatgatt
    15301 cttattatca atattatggc aggccagagc aagcagtaat gtgttgaaaa gacaacaagt
    15361 tgcgcagaaa tgcccatccc atcaggaatg ggcacgcatt cagaagcggg tatctaccgc
    15421 agaggcgagt ttttcgacca ggcgttcggt atcttcccag cccagacacg ggtcggtaat
    15481 ggattgaccg taagtgagcg gctgaccgcc gacgattttt tgcgttcctt cgcgcaggaa
    15541 actttccgcc ataattccag caatcgccgt agagccattg cggatttgct gacaaatatc
    15601 ctcacaaact tctaactggc gacggtgctg cttctggcag ttaccgtggc tgaaatccac
    15661 caccagatgt tcaggtaaat caaactcgtg cagcgtatcg caggctgcgg cgatatcatc
    15721 ggcatgataa ttcggttttt tgccgccacg cataataatg tggccatacg ggttgccgct
    15781 ggtctgatag atggtcatct gaccattttt gtctggcgag agaaacatat ggctggcgcg
    15841 ggctgcgcgg atagcatcca cggcaatccg cgtgttgcca tcggtaccat ttttaaaacc
    15901 taccggacag gagagtgccg aagccatttc acggtggatc tgactttcgg tagtacgtgc
    15961 gccaatcgcg ccccaactga ttaaatcagc aataaactga ccggtcacca tatcgaggaa
    16021 ctcagtcgcg gtagggacac ccagctcatt tacctgtaaa agtaatttgc gcgccagctc
    16081 cagaccgtga tttacccgat agctgccgtt taaatccgga tcggagatta gccctttcca
    16141 gccgacaaca gttcgtggtt tttcaaaata ggtgcgcatt acgatttcca gccgtgactg
    16201 gtactggttg cgcagcgact gcagacgggt ggcgtactcc attgcagcgg tgagatcgtg
    16261 gatcgagcag gggccaataa taaccaacag tcgcttatct tcaccattca gtattttttc
    16321 aattctgcgg cgggagtcgg tgacatgggt ggcgacgcca ggcgttacgg gataccgtag
    16381 cgcgagttcg gcgggcgtta ccaggctctc aatacgcgca gtacggagtt cgtcagttct
    16441 gttcattaca agtctcagaa atttgttgct tctacggcag gactaccggg aagtgatgcg
    16501 catcacgata aaccaatcgc agctgatttc aagtgcgagg tgtgtcacac ctcgccgggt
    16561 ggttagcatg ataacaaaaa aataagctaa tgcactagtt ctctagtaca ttcggcggct
    16621 aagccccatg atatcgagga tcttggtggc aatctcttct accgaataat tggtactgtt
    16681 aatccacggg atctgatttt tacggtacaa cgcttctact tccgcgactt ccatcctgca
    16741 ctgacgaagc gaggcatagc ggctgttctc ccgacgttcc tcgcgaatcg ccgccagacg
    16801 ttccgggtcg atagtcaggc cgaacaattt atgctgaagc ggtttgagcg acgcgggtag
    16861 caccagatta tccatatcgt cggcaataaa ggggtagttt gccgcgcgga taccaaattg
    16921 cattgccaga tacagactgg tgggggtttt accacagcga gaaacgccga gcaggatcac
    16981 ctgagcctgg tccagattgc gcaacgaaat accgtcatcg tgggcaaggg tgtaatcaat
    17041 cgccgcaatg cgcgcatcat atttattgag attattaggg ttaaggccat gggtacgatg
    17101 agcaatcggc gttggatcca gtttcatctc ttgttgtagc ggggcaacca gcgcctgaac
    17161 gatatcctgg caaaagcctt cactttgcaa gatgatggcg cgaatctccg gcaacacgat
    17221 ggagtagaag accagcgggc gcacacctgt ctggtgataa atggcgtcaa tctgatcctt
    17281 cactgcccgt gcacggctct cattttcgac aaacggcagc gtaatgctgc tgatagtgac
    17341 gggaaattgt gacattactg cgtgtcctaa tacctccgca gttattgccg taccatcaga
    17401 aatataaaaa acgtggcgat caacagcatt atccattttg ttcttcccgt gatgcagaca
    17461 taattctatg aaagcataaa ttaaaaacgc acagaagcgt aggacgtaat gtctggttta
    17521 taaaatgaac cttcaatttt attttttatg aaaacagtat ttcattttta tggtttcgtt
    17581 tataccgatg gtttatgtgg aaattgtcga agagagcaga tttgcgcaac gctgggatca
    17641 gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt catccggtta
    17701 aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta tcacaaaagg
    17761 attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac caactcggca
    17821 tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg attactaacc
    17881 tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac gcgtttaacc
    17941 agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat aaaacggata
    18001 ttgacgatgt tactcagctt gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca
    18061 ctcccttcca gcctgagctg gaaaacgcca tccgcgaagc ctatgcacag ctttccgccg
    18121 atgacgaaaa cgcctcgttt gcggtgcgtt cctccgctac tgcagaagat atgccggacg
    18181 cttcttttgc cggtcagcag gaaaccttcc tcaacgttca gggttttgac gccgttctcg
    18241 tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc
    18301 accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcagcgg atggtgcgct
    18361 ccgacctcgc atcttctggc gtgatgttct ccattgatac cgaatccggc tttgaccagg
    18421 tggtgtttat cacttccgca tggggccttg gggaaatggt cgtgcagggt gcggttaacc
    18481 cggatgagtt ttacgtccat aaaccgacac tggcggcgaa tcgcccggct atcgtgcgcc
    18541 gcaccatggg gtcgaaaaaa atccgcatgg tttatgcgcc gacccaggag cacggcaagc
    18601 aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg accaacgaag
    18661 aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt cgcccaatgg
    18721 atattgagtg ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag gcgcgtccgg
    18781 aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca cagggtaaga
    18841 ttatcgccga aggccgtgct atcggtcatc gcatcggtgc gggtccggtg aaagtcatcc
    18901 atgacatcag cgaaatgaac cgcatcgaac ctggtgacgt gctggtcact gatatgaccg
    18961 acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac cgtggcggtc
    19021 gtacctgtca cgcggcgatc atcgctcgtg aactgggcat tccggcggta gtgggctgtg
    19081 gagatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg
    19141 ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa
    19201 cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggac cgtgctttcg
    19261 acttcgcctg tctgccgaac gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc
    19321 gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc
    19381 aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc
    19441 gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc
    19501 gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag cgttacgagc
    19561 cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg ccgctatgtt tccgacagct
    19621 tccgcgactg tttcgcgctg gagtgtgaag cagtgaaacg tgtgcgcaat gacatggggc
    19681 tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg
    19741 ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa atcatcatga
    19801 tgtgtgaaat cccgtccaac gccttgctgg cagagcagtt cctcgaatat ttcgacggct
    19861 tctcaattgg ctcaaacgac atgacacagc tggcgctcgg tctggaccgt gactccggag
    19921 tggtgtctga actgttcgat gagcgcaacg atgcggtgaa agcactgctg tcgatggcga
    19981 ttcgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc
    20041 atgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct ctgaacccgg
    20101 acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg
    20161 cggcgttcag tcgccgggtt tatgtgatcc ccgaagatga aacttattca atctcttcac
    20221 agacatcctg cgttaatcgc cgcataatat cttttcttaa caaaaacttt tgtattttac
    20281 ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat ttatattttg
    20341 cgacccgttt acggctaaaa aaagccacca cctcttccag cgataatgaa tgatgcggcg
    20401 ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc attgcaacca
    20461 cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca cggctgctaa
    20521 tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt atatagccag
    20581 cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc agcgcacggg
    20641 cggttaattc aggttcatca aaatatccca taaacacatt ggggccacgc gaggcttctt
    20701 caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta atctctacac
    20761 ctgcggcagc gtaaccatcg gtgtgcataa agcgcgacaa aggatcatcg agattcacca
    20821 ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg ccgagctgct
    20881 ggcattcacg cgccactttt ttggggattg tggttccgcc gcaaagaaag aaacgcagcg
    20941 ctgacaggtc cgcgggttgt ttctctagta cattcaaaag atcatagaca aacggcgttg
    21001 cgcctagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca tcaggagtga
    21061 aaatatctaa caacacgctg cgagcgccaa ttaagaatgg tgccgttacg ccatgcagaa
    21121 agcccgttgc gtggccgagt ggcgcaggca tcataaagac atcctgccag gtcagattca
    21181 gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc atcacgccct
    21241 ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg
    21301 ttatcgccgt ggtcagtggg gtattgtcgg cgataatctg acttaatgag agggaagagg
    21361 tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc tgattttgca
    21421 gcggcaggat taaatctacc gggcgcgttt gtttaaacaa cgtcggtgca aagaacattt
    21481 ttgcctgaca cttattgagc acccacacca gttctgcttc ccgccaggaa ggcaacagcg
    21541 gcacggaaac cgcaccgatt ttcaggcagg caagatagat aacggtaaat tcacaccagc
    21601 caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac atccagtttg
    21661 ccagacagct cgcggcgtga tcgagcgcgc tatagttgta cgatgcacca tgattatcga
    21721 ccacggcaat tttgtctggc atcgcacgag cggtctgctg ccagtaatcg gccagcgaag
    21781 catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg
    21841 tcactttcat gttggactct cgaaacagaa catcaggccc gagatgcggg cctgtgggat
    21901 gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt gccactgttc
    21961 gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac cggcagaatc
    22021 aaaatgaatg ttgccggcgt catcctgctt ataaagtccg gcagggcagg ctttcattaa
    22081 tttacggaat tcattgatat cgggatttgc cgccagaatg atatgcgggt ggccctcatc
    22141 aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt tctggctcat
    22201 agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat tttcttcgca
    22261 tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt gaacatctcg
    22321 ttcatgatgt cggcgaccat tcgtgggtat tggctaaaca ggcgcgggtt ttccatcagc
    22381 gccgggatct tgcggaaatg ctgcatatcg cgcatgacgc agctttgttc cagctcacgt
    22441 ttgtattgcg ccagactgct ggcggagaaa tcctcgcgtt ctttggcggc gatcaccgtt
    22501 gtggcggcag cctgagccga tgcaatggct aaatccatgc cacgaactgt aaaacccaaa
    22561 ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc catcgttaac catctgcggc
    22621 accattgcca gaccgccttc tggcaccata tgcccggaat attcaagcag tttgccgccg
    22681 ctaattagtg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt
    22741 ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat ggaatccttg
    22801 ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc aaacagccag
    22861 gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg tgtgaggcca
    22921 ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac cattcccagc
    22981 gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc ttcgagaata
    23041 tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc atcgacgcga
    23101 acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga
    23161 cgattacgca atacggtata agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa
    23221 tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt
    23281 tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg ggcataaaga
    23341 cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac caggacatcc
    23401 agcccggctc gcgccatgac cagtgcggca acgctaccag caacgcccgc accgaccaca
    23461 atggcatcaa atttgtcttc cgacatgcgt tatgctccct tcccgctgcg tttacagcgg
    23521 gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag ggacgacttt
    23581 gtagatatcg cccaccagac cgtagtcggc ataattgaag attggcgcat ttttatcttt
    23641 attgatggcg acaatcactt ttgcgccgtt gccgccaacc atatgctgga tctgcccgga
    23701 gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac cgatataacg
    23761 ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga cttcagcatt
    23821 cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca gaccacgacc
    23881 gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt tcgcacggcg
    23941 ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg tcgggcactg
    24001 atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa tgatcgccag
    24061 cgggctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt gttcggcgca
    24121 aatgtgacca tcgacaatat ccaccgccgt ggcatcattc accagcgccg cattcagttg
    24181 cacacttaac cgtgctgcca gagctttacc acgtttcgtc gcggccaaca gcaacatagc
    24241 ggggtgttta tctttcagca gggcagcaat gctttcggcg taattttcag tgcgttgcag
    24301 cgcgtcgttt tgctcaagaa cataaataca ttttggacca taaggcataa ctgcctgcgc
    24361 ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag cgccgccaaa
    24421 cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta attgactcat
    24481 cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc agctcggcaa
    24541 tggcctccgg cgaatcgtta tcgataatga tgtgcttacg ttctgtttgc ggcggtacgc
    24601 gaatgccgac cagttcagca agtggcgcgc tctggctcca gtcaatatca cttgcctgcc
    24661 actgatttac cggtttttta cccgcgccga gaatggcttt catcgaagga atgcgtggca
    24721 cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata acttcaacat
    24781 catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata gcactcactg
    24841 cattaatcac cggaagttgc agaatttctc cgaccagcaa gccagcctgc tgggcataaa
    24901 ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca actgctgctg
    24961 ccagcgcttt tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc tgaaccaaat
    25021 acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg
    25081 agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcaagctgg cttgcagctt
    25141 caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt
    25201 attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag gttattattt
    25261 tcatgatttc tcctgtgagt cgcatacgga accatctccg atggaaggta aaaatggcct
    25321 gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag caagctgttt tccttgccaa
    25381 caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc aatttgcggc aatatagtta
    25441 ttccatgacc gtacaatctt gcattcagtg taaataatct tgtttagctc tcgctaaacg
    25501 cggttgtata accgcaatac acgccgtatt ttatccggca tatattgttt ttgcttgctc
    25561 atggaatagg gaggttgtca tgtatcaacg ctgttttgat aatgccagtg aaacgctgtt
    25621 tgtcgccggt aaaacgccac ggctttcacg ttttgcaatt agcgatgatc caaaatggga
    25681 gtctggacat cacgttcatg acaatgaaac cgagctgatt tacgtcaaga aaggggtagc
    25741 aaggtttacc atcgattcat cgttatatgt cgcacatgcc gatgatatag tggtgataga
    25801 acgcggcagg ctgcatgcgg tggcctctga cgttaacgat ccggcaacga cgtgtacctg
    25861 tgcgctgtac ggctttcagt ttcagggggc tgaggaaaat cagctactgc aaccgcattc
    25921 ttgtccggta attgccgcgg ggcagggaaa agaagtcata aaaaccttat ttaatgagct
    25981 aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct tcgttatggg atgcatttgc
    26041 ctatacgtta gccattcttt actacgaaaa ctttaaaaat gctta
//
LOCUS       DAZFVO020000060        26060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFVO020000060 DAZFVO020000000
VERSION     DAZFVO020000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.060"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKNB_RS11515"
                     /old_locus_tag="pgaptmp_002303"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKNB_RS11515"
                     /old_locus_tag="pgaptmp_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HGU4195802.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKNB_RS11520"
                     /old_locus_tag="pgaptmp_002304"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKNB_RS11520"
                     /old_locus_tag="pgaptmp_002304"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HGU4195803.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKNB_RS11525"
                     /old_locus_tag="pgaptmp_002305"
     CDS             complement(941..1408)
                     /locus_tag="ACHKNB_RS11525"
                     /old_locus_tag="pgaptmp_002305"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGU4195804.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKNB_RS11530"
                     /old_locus_tag="pgaptmp_002306"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKNB_RS11530"
                     /old_locus_tag="pgaptmp_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGU4195805.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKNB_RS11535"
                     /old_locus_tag="pgaptmp_002307"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKNB_RS11535"
                     /old_locus_tag="pgaptmp_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGU4195806.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKNB_RS11540"
                     /old_locus_tag="pgaptmp_002308"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKNB_RS11540"
                     /old_locus_tag="pgaptmp_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGU4195807.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKNB_RS11545"
                     /old_locus_tag="pgaptmp_002309"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKNB_RS11545"
                     /old_locus_tag="pgaptmp_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGU4195808.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKNB_RS11550"
                     /old_locus_tag="pgaptmp_002310"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKNB_RS11550"
                     /old_locus_tag="pgaptmp_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGU4195809.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKNB_RS11555"
                     /old_locus_tag="pgaptmp_002311"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKNB_RS11555"
                     /old_locus_tag="pgaptmp_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGU4195810.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKNB_RS11560"
                     /old_locus_tag="pgaptmp_002312"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKNB_RS11560"
                     /old_locus_tag="pgaptmp_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGU4195811.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKNB_RS11565"
                     /old_locus_tag="pgaptmp_002313"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKNB_RS11565"
                     /old_locus_tag="pgaptmp_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGU4195812.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKNB_RS11570"
                     /old_locus_tag="pgaptmp_002314"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKNB_RS11570"
                     /old_locus_tag="pgaptmp_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGU4195813.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKNB_RS11575"
                     /old_locus_tag="pgaptmp_002315"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKNB_RS11575"
                     /old_locus_tag="pgaptmp_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGU4195814.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKNB_RS11580"
                     /old_locus_tag="pgaptmp_002316"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKNB_RS11580"
                     /old_locus_tag="pgaptmp_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGU4195815.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKNB_RS11585"
                     /old_locus_tag="pgaptmp_002317"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKNB_RS11585"
                     /old_locus_tag="pgaptmp_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGU4195816.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKNB_RS11590"
                     /old_locus_tag="pgaptmp_002318"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKNB_RS11590"
                     /old_locus_tag="pgaptmp_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGU4195817.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKNB_RS11595"
                     /old_locus_tag="pgaptmp_002319"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKNB_RS11595"
                     /old_locus_tag="pgaptmp_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGU4195818.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKNB_RS11600"
                     /old_locus_tag="pgaptmp_002320"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKNB_RS11600"
                     /old_locus_tag="pgaptmp_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGU4195819.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKNB_RS11605"
                     /old_locus_tag="pgaptmp_002321"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKNB_RS11605"
                     /old_locus_tag="pgaptmp_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGU4195820.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKNB_RS11610"
                     /old_locus_tag="pgaptmp_002322"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKNB_RS11610"
                     /old_locus_tag="pgaptmp_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGU4195821.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKNB_RS11615"
                     /old_locus_tag="pgaptmp_002323"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKNB_RS11615"
                     /old_locus_tag="pgaptmp_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGU4195822.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKNB_RS11620"
                     /old_locus_tag="pgaptmp_002324"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKNB_RS11620"
                     /old_locus_tag="pgaptmp_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGU4195823.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKNB_RS11625"
                     /old_locus_tag="pgaptmp_002325"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKNB_RS11625"
                     /old_locus_tag="pgaptmp_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGU4195824.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKNB_RS11630"
                     /old_locus_tag="pgaptmp_002326"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKNB_RS11630"
                     /old_locus_tag="pgaptmp_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGU4195825.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKNB_RS11635"
                     /old_locus_tag="pgaptmp_002327"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKNB_RS11635"
                     /old_locus_tag="pgaptmp_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGU4195826.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKNB_RS11640"
                     /old_locus_tag="pgaptmp_002328"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKNB_RS11640"
                     /old_locus_tag="pgaptmp_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGU4195827.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKNB_RS11645"
                     /old_locus_tag="pgaptmp_002329"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKNB_RS11645"
                     /old_locus_tag="pgaptmp_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGU4195828.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKNB_RS11650"
                     /old_locus_tag="pgaptmp_002330"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKNB_RS11650"
                     /old_locus_tag="pgaptmp_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGU4195829.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKNB_RS11655"
                     /old_locus_tag="pgaptmp_002331"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKNB_RS11655"
                     /old_locus_tag="pgaptmp_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGU4195830.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKNB_RS11660"
                     /old_locus_tag="pgaptmp_002332"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKNB_RS11660"
                     /old_locus_tag="pgaptmp_002332"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGU4195831.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKNB_RS11665"
                     /old_locus_tag="pgaptmp_002333"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKNB_RS11665"
                     /old_locus_tag="pgaptmp_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGU4195832.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKNB_RS11670"
                     /old_locus_tag="pgaptmp_002334"
     CDS             15548..15916
                     /locus_tag="ACHKNB_RS11670"
                     /old_locus_tag="pgaptmp_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HGU4195833.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKNB_RS11675"
                     /old_locus_tag="pgaptmp_002335"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKNB_RS11675"
                     /old_locus_tag="pgaptmp_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HGU4195834.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKNB_RS11680"
                     /old_locus_tag="pgaptmp_002336"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKNB_RS11680"
                     /old_locus_tag="pgaptmp_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HGU4195835.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKNB_RS11685"
                     /old_locus_tag="pgaptmp_002337"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKNB_RS11685"
                     /old_locus_tag="pgaptmp_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGU4195836.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKNB_RS11690"
                     /old_locus_tag="pgaptmp_002338"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKNB_RS11690"
                     /old_locus_tag="pgaptmp_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGU4195837.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKNB_RS11695"
                     /old_locus_tag="pgaptmp_002339"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKNB_RS11695"
                     /old_locus_tag="pgaptmp_002339"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGU4195838.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKNB_RS11700"
                     /old_locus_tag="pgaptmp_002340"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKNB_RS11700"
                     /old_locus_tag="pgaptmp_002340"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGU4195839.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKNB_RS11705"
                     /old_locus_tag="pgaptmp_002341"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKNB_RS11705"
                     /old_locus_tag="pgaptmp_002341"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGU4195840.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKNB_RS11710"
                     /old_locus_tag="pgaptmp_002342"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKNB_RS11710"
                     /old_locus_tag="pgaptmp_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HGU4195841.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHKNB_RS11715"
                     /old_locus_tag="pgaptmp_002343"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHKNB_RS11715"
                     /old_locus_tag="pgaptmp_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HGU4195842.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHKNB_RS11720"
                     /old_locus_tag="pgaptmp_002344"
     CDS             23095..23637
                     /locus_tag="ACHKNB_RS11720"
                     /old_locus_tag="pgaptmp_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HGU4195843.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKNB_RS11725"
                     /old_locus_tag="pgaptmp_002345"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKNB_RS11725"
                     /old_locus_tag="pgaptmp_002345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HGU4195844.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKNB_RS11730"
                     /old_locus_tag="pgaptmp_002346"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKNB_RS11730"
                     /old_locus_tag="pgaptmp_002346"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGU4195845.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHKNB_RS11735"
                     /old_locus_tag="pgaptmp_002347"
     CDS             25034..25291
                     /locus_tag="ACHKNB_RS11735"
                     /old_locus_tag="pgaptmp_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HGU4195846.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHKNB_RS11740"
                     /old_locus_tag="pgaptmp_002348"
     CDS             complement(25267..25821)
                     /locus_tag="ACHKNB_RS11740"
                     /old_locus_tag="pgaptmp_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HGU4195847.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6697 a         6184 c         6751 g         6428 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgccaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aggtataaaa
    19861 atgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttattg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
    20101 actttaaccg gttgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
    20161 atgcagcgtt caagctgcgc tgccgaaagc gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcaca gctttaggca gaattaaacc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaat cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccatctg
    21001 cctccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcata catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa ctgggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg aaccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag
//
LOCUS       DAZFVO020000061        25642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFVO020000061 DAZFVO020000000
VERSION     DAZFVO020000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.061"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            232..651
                     /locus_tag="ACHKNB_RS11745"
                     /old_locus_tag="pgaptmp_002349"
     CDS             232..651
                     /locus_tag="ACHKNB_RS11745"
                     /old_locus_tag="pgaptmp_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4195848.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            651..1919
                     /gene="umuC"
                     /locus_tag="ACHKNB_RS11750"
                     /old_locus_tag="pgaptmp_002350"
     CDS             651..1919
                     /gene="umuC"
                     /locus_tag="ACHKNB_RS11750"
                     /old_locus_tag="pgaptmp_002350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HGU4195849.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1965..2495)
                     /gene="dsbB"
                     /locus_tag="ACHKNB_RS11755"
                     /old_locus_tag="pgaptmp_002351"
     CDS             complement(1965..2495)
                     /gene="dsbB"
                     /locus_tag="ACHKNB_RS11755"
                     /old_locus_tag="pgaptmp_002351"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HGU4195850.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2641..4182)
                     /gene="nhaB"
                     /locus_tag="ACHKNB_RS11760"
                     /old_locus_tag="pgaptmp_002352"
     CDS             complement(2641..4182)
                     /gene="nhaB"
                     /locus_tag="ACHKNB_RS11760"
                     /old_locus_tag="pgaptmp_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HGU4195851.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4403..5122
                     /gene="fadR"
                     /locus_tag="ACHKNB_RS11765"
                     /old_locus_tag="pgaptmp_002353"
     CDS             4403..5122
                     /gene="fadR"
                     /locus_tag="ACHKNB_RS11765"
                     /old_locus_tag="pgaptmp_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HGU4195852.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5174..6706)
                     /locus_tag="ACHKNB_RS11770"
                     /old_locus_tag="pgaptmp_002354"
     CDS             complement(5174..6706)
                     /locus_tag="ACHKNB_RS11770"
                     /old_locus_tag="pgaptmp_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HGU4195853.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7036..8334
                     /gene="dadA"
                     /locus_tag="ACHKNB_RS11775"
                     /old_locus_tag="pgaptmp_002355"
     CDS             7036..8334
                     /gene="dadA"
                     /locus_tag="ACHKNB_RS11775"
                     /old_locus_tag="pgaptmp_002355"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HGU4195854.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8344..9414
                     /gene="dadX"
                     /locus_tag="ACHKNB_RS11780"
                     /old_locus_tag="pgaptmp_002356"
     CDS             8344..9414
                     /gene="dadX"
                     /locus_tag="ACHKNB_RS11780"
                     /old_locus_tag="pgaptmp_002356"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HGU4195855.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9800..11536)
                     /locus_tag="ACHKNB_RS11785"
                     /old_locus_tag="pgaptmp_002357"
     CDS             complement(9800..11536)
                     /locus_tag="ACHKNB_RS11785"
                     /old_locus_tag="pgaptmp_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HGU4195856.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11631..12545)
                     /gene="ldcA"
                     /locus_tag="ACHKNB_RS11790"
                     /old_locus_tag="pgaptmp_002358"
     CDS             complement(11631..12545)
                     /gene="ldcA"
                     /locus_tag="ACHKNB_RS11790"
                     /old_locus_tag="pgaptmp_002358"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HGU4195857.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12645..13256
                     /gene="emtA"
                     /locus_tag="ACHKNB_RS11795"
                     /old_locus_tag="pgaptmp_002359"
     CDS             12645..13256
                     /gene="emtA"
                     /locus_tag="ACHKNB_RS11795"
                     /old_locus_tag="pgaptmp_002359"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HGU4195858.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13258..13992)
                     /locus_tag="ACHKNB_RS11800"
                     /old_locus_tag="pgaptmp_002360"
     CDS             complement(13258..13992)
                     /locus_tag="ACHKNB_RS11800"
                     /old_locus_tag="pgaptmp_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HGU4195859.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14193..14447
                     /locus_tag="ACHKNB_RS11805"
                     /old_locus_tag="pgaptmp_002361"
     CDS             14193..14447
                     /locus_tag="ACHKNB_RS11805"
                     /old_locus_tag="pgaptmp_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4195860.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14625..15065
                     /gene="ycgY"
                     /locus_tag="ACHKNB_RS11810"
                     /old_locus_tag="pgaptmp_002362"
     CDS             14625..15065
                     /gene="ycgY"
                     /locus_tag="ACHKNB_RS11810"
                     /old_locus_tag="pgaptmp_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HGU4195861.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSCRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15144..16841)
                     /gene="treA"
                     /locus_tag="ACHKNB_RS11815"
                     /old_locus_tag="pgaptmp_002363"
     CDS             complement(15144..16841)
                     /gene="treA"
                     /locus_tag="ACHKNB_RS11815"
                     /old_locus_tag="pgaptmp_002363"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HGU4195862.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GSLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17161..18579)
                     /gene="dhaM"
                     /locus_tag="ACHKNB_RS11820"
                     /old_locus_tag="pgaptmp_002364"
     CDS             complement(17161..18579)
                     /gene="dhaM"
                     /locus_tag="ACHKNB_RS11820"
                     /old_locus_tag="pgaptmp_002364"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGU4195863.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(18590..19222)
                     /gene="dhaL"
                     /locus_tag="ACHKNB_RS11825"
                     /old_locus_tag="pgaptmp_002365"
     CDS             complement(18590..19222)
                     /gene="dhaL"
                     /locus_tag="ACHKNB_RS11825"
                     /old_locus_tag="pgaptmp_002365"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HGU4195864.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19233..20303)
                     /gene="dhaK"
                     /locus_tag="ACHKNB_RS11830"
                     /old_locus_tag="pgaptmp_002366"
     CDS             complement(19233..20303)
                     /gene="dhaK"
                     /locus_tag="ACHKNB_RS11830"
                     /old_locus_tag="pgaptmp_002366"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HGU4195865.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20531..22450
                     /gene="dhaR"
                     /locus_tag="ACHKNB_RS11835"
                     /old_locus_tag="pgaptmp_002367"
     CDS             20531..22450
                     /gene="dhaR"
                     /locus_tag="ACHKNB_RS11835"
                     /old_locus_tag="pgaptmp_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HGU4195866.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22550..25417)
                     /locus_tag="ACHKNB_RS11840"
                     /old_locus_tag="pgaptmp_002368"
     CDS             complement(22550..25417)
                     /locus_tag="ACHKNB_RS11840"
                     /old_locus_tag="pgaptmp_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4195867.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNG
                     VKANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
BASE COUNT         6339 a         6377 c         6593 g         6333 t
ORIGIN      
        1 caatgtttaa tgtcattatg gcgaatgctt ctattctatt tttttgccgg gtgatatttt
       61 tcatttctgc tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg
      121 aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg
      181 gcaagaacag actactgtat ataaaaacag tataacttca ggcagattat tatgttgttt
      241 atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag
      301 tgtggctttc cttcaccggc agcagattac gttgaacagc gcatcgatct gaatcaactg
      361 ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat
      421 ggtggaatta gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt
      481 gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg
      541 acggtacagc ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat
      601 acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgctc
      661 tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg
      721 gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg
      781 caaaggcgct gggtgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc
      841 gctgtggcgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg agcaatcggg
      901 tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat
      961 tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac tgattttggc agagaaattc
     1021 gcgcaacggt gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa
     1081 cgttggctaa gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggtggggtgg
     1141 tggatttatc aaatctggaa cgccagcgta aattaatgtc tgctctcccc gtggatgacg
     1201 tctgggggat tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc
     1261 tcgatttggc ggatacagat atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa
     1321 gaacggtgcg tgaactgcgc ggcgaaccct gtttgcaact ggaagagttt gcaccgacga
     1381 agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat cacggattat ccatcgatgc
     1441 ggcaggccat ttgtagttac gctgcccggg cggcggaaaa acttcgcagc gagcatcaat
     1501 attgtcggtt tatctccaca tttattaaga cgtcaccatt tgcgctcaat gaaccttatt
     1561 acggcaatag cgcgtcggta aaactgctga cgcccactca ggacagcagg gatatcatta
     1621 acgctgctac gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg
     1681 gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca
     1741 acgcaccgcg ccccgggagt gagcaattga tgacggtaat ggatacactg aatgctaaag
     1801 agggcagagg aacactctat tttgccgggc aggggatcca gcaacaatgg cagatgaagc
     1861 gtgccatgct ttcaccacgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa
     1921 tatagcggca ggaaaaaagc gctcccgcag gagcgctgaa gggattagcg accgaacagg
     1981 tcacgttttt tcgctttaaa cggctgggaa atcaccacca gcaccgcgac aatcaggtaa
     2041 gcgataaaaa taccgagcag ccactgcggc atttccatac ctaaaaaatc ccactgacgc
     2101 tcggcacaat cgccagaggc gacaaacact tgcggcaccc atttatccag cggcagccat
     2161 tccgggaaac gaaccataaa atcacaggtg gcaaacggcg aaggatagag ctgaagcatg
     2221 gtgtgctcgt aagttaactg cacaccgcgg aacgcactat acaaccagat aaccatcgct
     2281 acataacgca gcggagtttt cggggcgatc gcgccaatca gcgcagcacc cagaacgccg
     2341 aataacgcac aacgttcata aatacagagc acgcaaggtt tcagtaacat cacatgctgg
     2401 aaccacagcg ccgtcagttc cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc
     2461 cggccttgtg aacattggtt caaaaatcgc aacataatca tttccctgca atatgcatag
     2521 agtgcgcagt ttaaaccaat tcattcgctg cgccaccagg aggcagcaaa aataggaagc
     2581 aattggacaa ttatcgggcg aaaaggcaaa ccgggcattt atatgcccgg taagttgtta
     2641 tcaaagcgtt gctatccagc ccatttgcat aaaccattcg gttacagggg caagcgtaaa
     2701 ctcgacgcag agcaagccga cgagtgtcag tacgagggtg taaggcaggg ccatccacac
     2761 catgcggcca taagagaggc gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa
     2821 cgcagcctga ccgttcggcg tagcgacgga gggcagattg gtaccggtat taatggcgac
     2881 cgccagcagc tcgtattgct tcaacgtgat agcgccactt tccattgccg ctttcgcttc
     2941 gttgatataa atcgtcccca cgaagacgtt atccgaaatg gatgacagca gaccgttgaa
     3001 aatatagaac agcgacagct gagcatgttc cgatgcctgc aacacaaact gaataattgg
     3061 cgaaaacagt tgttggtcga taatcaccgc gactaccgag aaaaacaccg tcaacagtgc
     3121 ggtgaatggc agagattcgg tgaaggcttt accgatagca tgttcatcgg tgacaccggt
     3181 caatgatgtc gccagaataa tgactgacaa accaatcaag ccaacttccg ccagatgcaa
     3241 cgccagcgca gtcaccagcc agacgccaat aatcgcctgg acaatcagac ggattttatc
     3301 ctgacgggta cgctggtggc ggctttgatc gtcaaactgt tgcaacactt cgcggacttt
     3361 ctccggcagc gtttcaccgt agccaaacca acgcagcttc tctaccagca ggcaggttaa
     3421 caggccacaa atcagaaccg gaacggtcac cggcgacatg cgcaggaaga aatcgccaaa
     3481 atgccagcca gccgctttag cgatgatcag gttctgtggt tcgcccacca tggtcatcac
     3541 gccgcctaat gcagtgccga caccggcatg catcatcagg ctacgcagaa agccacggaa
     3601 ctgttccaga accactttgt aatgcttgtc gatatgacta tcgtcttgca ggtcggtatc
     3661 ttcagtacgg gaagaggcta cgcggtgata aataccataa aaaccgactg caacgctgat
     3721 caccaccgcc acgacggtta aggcatcgag gaacgcggag aggaacgccg ccgccacgca
     3781 aaaagagagc gacagcagca ttttggagcg aatgcttaac agcaaacggg taaatatgaa
     3841 cagcaacagc tgtttcataa aatagatacc cgccaccata aacatcaata acagcaagac
     3901 ttcaagattt gccgccacct cttcacggat gtgttccgcg ctggtcatgc cgatgaatac
     3961 cgcttcgata gccaacagac caccggggag tagcgggtag catttcaggg ccatcgccag
     4021 agtgaaaata aattccgcta ccagcaacca gcccgcgacg aaagggctga tgaggaaaat
     4081 taacgggttt acgattaaga aaatgatgag ggcgagtttg taccagtcgg gggactggcc
     4141 caaaaaattg cgccatagcg cgcggcccca ggagatctcc atgatggttt cccttacctt
     4201 acaaataatc aatgatgttt ttatgtttaa acgcaaagct taacggtcag gcaggagtga
     4261 ggcaagtctt gatagtcaag gggaaagaga tgcggaaaat gaagccttga tccctttttc
     4321 ttctttttgt ctgctatcag cgtagttagc cctctggtat gatgagtcca actttgtttc
     4381 gctgtgttat ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga
     4441 agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc
     4501 agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca
     4561 gcgtctggca cgagatggct ggttgaccat tcaacatggc aagccgacga aggtgaataa
     4621 tttctgggaa acctctggtt taaatatcct tgaaacactg gcgcgactgg atcacgaaag
     4681 tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcca ctatttttat
     4741 tcgcaccgcg tttcgtcagc atcccgataa agcgcaggaa gtgctggcta ccgctaatga
     4801 agtggccgat cacgccgatg cctttgccga gctggattac aacatattcc gcggcctggc
     4861 gtttgcttcc ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac
     4921 gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta
     4981 ccacaaactg tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg
     5041 tcgctatggg catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga
     5101 tttagccatt caggggcgat aatcccttcc gtttaaagag caaacccctc aaacgagggg
     5161 ttttttgttg tttttacaga tttcccattc ttggcgggca acgttccagc aactcgatgc
     5221 tgccgtcttc gttttgctgt tcgagcatca catcaaatcc ccacaggcga tgcacatgct
     5281 tcagcacttc tttgcgcccc cgatccagcg gtgcgcgatt atgtggaatg taacgcagcg
     5341 tcagcgaacg gtcgccgcgc aaatccacgt tccagatctg aatattcggc tccagattac
     5401 ttaagttata ttgcgacgat aaccggttac ggatctcccg ataaccttct tcattatgaa
     5461 tagcggaaat ctccagataa ttatgccgat catcatccag cacggtgaag aagcggaaat
     5521 cacgcatcac tttcggtgac agaaactggc tgataaaact ctcatctttg aaatcacgca
     5581 tcgcgaaatg cagcgtttcc agccagtcgg aaccggcgat atccgggaac cagtatttgt
     5641 cttcttccgt tggcgactga caaatccgtt taatatcctg gaacatggcg aacccgaggg
     5701 catacgggtt gatgccgctg taccacgggc tgttataggg gggctggaag accacattgg
     5761 tgtggctgtg caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat
     5821 ggttaaggat ggtatagtgc cagaaggtcg cccagccttc gttcatcacc tgagtctgtt
     5881 tttgcggata aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg
     5941 attccagcag tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg
     6001 gatagcggcg cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga
     6061 gcatattgac ctgactttgt agatactctt cgcgactttt ctgccgggct ttctcttctt
     6121 gcagcgagat tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc
     6181 acgagtccag aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat
     6241 atttacgggc aaaaatcaga taatcgacaa tcgaactggc gtcggtccaa ctgcggaata
     6301 agtaattgtt tttaaagaaa gagttatgcc catagcaggc gtgtgccatc accagcgcct
     6361 gcatggtaat ggtgttctct tccatcaggt aagcgataca cgggttagag ttaatgacga
     6421 tttcataggc cagtccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt
     6481 taccgaatga ccagtgcgga tagttaattg gcatgccgac gctggagtag gcatccatca
     6541 tctgttctga ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg
     6601 ccacccggtc tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat
     6661 cgctcaaacg tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt
     6721 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
     6781 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct
     6841 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
     6901 tattgccgca gagtcaggga gatgtgagcc agctcaccat aaaaaagccg tatgttgaat
     6961 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
     7021 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
     7081 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
     7141 gccctggaaa ccagcgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
     7201 gcggcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg
     7261 gcggttcgtc tcgacggtac gcagttccag ttgaaatgga tgtggcaaat gttacgtaac
     7321 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc
     7381 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
     7441 gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc
     7501 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
     7561 gagcccgcgc tggcagaagt agcgcacaaa ctgacgggtg gtctgcaact gcccaatgat
     7621 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcaga gcaggcgggg
     7681 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
     7741 ggcgtgaagt gtggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
     7801 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
     7861 tcgctgacta ttccaattgc gcaggaagat ggtgcgccag tatccaccat tcttgatgaa
     7921 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa
     7981 attgttggtt ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga aatggtggtt
     8041 cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtttgcgc
     8101 ccgatgacgc cagacggtac gccggttgtc gggcgtacac gctttaaaaa tctgtggctg
     8161 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
     8221 gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac
     8281 agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac
     8341 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
     8401 attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac
     8461 gggcatggta ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattactt
     8521 aacctggaag aggcaataac gttacgtgag cgcggctgga aggggccgat cctgatgctg
     8581 gaaggatttt tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc
     8641 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
     8701 atttatctta aagtgaacag tgggatgaat cggttgggct tccagcccga tcgcgtgctt
     8761 accgtctggc agcagttgcg ggcaatggcg aatgttggcg agatgaccct gatgtcgcat
     8821 tttgccgaag cggaacatcc tgatggaatt tccagcgcga tggcgcgtat tgagcaggcg
     8881 gcggaagggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg
     8941 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
     9001 cagtggcgtg atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt
     9061 attggtgtcc agacgctaaa agcgggtgag cgtgtgggct acggcggtcg ctatactgcg
     9121 cgcgatgaac agcgaatcgg cattgtcgcc gcagggtacg ccgacggtta tccgcgccac
     9181 gcgcctaccg gtacccctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc
     9241 tcgatggata tgctagcggt cgatttaacg ccttgcccgc aggcggggat tggtacgccg
     9301 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
     9361 ggctatgagt tgatgtgcgc gctggcgtta cgcgtcccgg ttgtgacagt gtaacttgtt
     9421 gtaagccgga tcggagacag cgtcttctgg ttgcgaaaaa atcatccatc cggctggtca
     9481 gcaactgtag ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaagaatg
     9541 gacactattt ccccggaacc tgaactcacc gcacaggcgt tctacataaa acgcttacgc
     9601 ttcattgttg actcgaactc gacttcagat aaatcacgct ttacccttga tggagcctgt
     9661 acatagattt gtgtaattgc ctgattttga tatgttcaat tcaacatcaa atgaaggtta
     9721 aattatggac gacaaacaat tgcaggctca ggctgcgttc agcaaagcat cgcaaccggc
     9781 gatagatgct tcattaaatt taagattcag cttcctcttc agccacccgt acgccaatct
     9841 tcaacacttc attatcttct ttctcggcca ccgtccagat catcccggca aactctacct
     9901 ggtcaccgac aaccggtgcc gcgcctaaca actgctggac aatttcaccc agcgtttgct
     9961 gcttatcacg atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt
    10021 tggcgctggc ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggcggcg
    10081 actggctgaa cagtttaccg agcgccggaa gatcgcgttc ccgaccaatt acacacaaca
    10141 catcgccttc gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaaca
    10201 gtgccgcaat acgcgtctct tttggcatat gcaaatcgcg cagtgccgcg cccacgcacc
    10261 atttatcggc actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca
    10321 ggccaacgcg tgacaccgga cgtcccactg gcggaaccac cactttggct tttttagccg
    10381 cccacgagag tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat
    10441 taaagaacag acgtgcattc tccagccccg ccatcatcgg gaacacggcc aggatgattg
    10501 gcaccgcgcc gcgtaatcct acccagctga taaacacgcg ctcacgcaga ttgaaaccgc
    10561 ggaaggggag caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg
    10621 acaaaatgag cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca
    10681 gcaccaggaa catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc
    10741 cgtagcggtt gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa
    10801 taccgctgcc ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca
    10861 gcaatggata taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac
    10921 caccgccaag tccgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca
    10981 tccaactgac actgctttca tgttgctgga tcatcgcaat cagggtaatc gtcagaaaga
    11041 ccgccattgg atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa
    11101 gccccttacc acccagcaga gaaaagaccg ctgcagcatc ggtagagccg acgatagcgc
    11161 cgattaataa gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta
    11221 aaccagaggt gataagcacg cccagcgtcg ccagcgacag tgccggtcct aacgccacac
    11281 gaaaggaact ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agtgccaggt
    11341 tacttaccat gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc
    11401 ctgccagcat gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa
    11461 atgaactaag taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta
    11521 ttgttgtggc atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac
    11581 catattagcg gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta
    11641 agaacaggat gaccgctaat cgtcaactga gtgccttccc gggtgttatt cagaatggca
    11701 tgtgcgccca acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg
    11761 agtggaatcg acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg
    11821 tcataatcat tgggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc
    11881 aaaattcccg catgatagag ctgcaacagc atacgttcga cgcggaaagg gtgctcgtta
    11941 atatcttcca gcaccagaat accgttctca atttttggca tccacggtgt accaatcagt
    12001 gaaatcagca tcgcaagatt gcctccccac aacgtgcctt cggtctgaca tgtcgggcct
    12061 tccccttgcc attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg
    12121 gtaaaggcgt tcagttcatc cgcgccaaag tttgctacca gcatggggcc gctaaaggtg
    12181 atgacattgc cttgcgccag aagaccgcac tgaatggcgg taaaatcgct atgcccgcat
    12241 atgagcaacg ggtcatattg ttggcgagca accagtgcct gccagtcaat atctgccagt
    12301 aaacgactgg caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtggtcagt
    12361 ctggcaaggg aattgagatc ttccaggcgc tccgtttccg tacccgcaaa acgctcacaa
    12421 cgacgggcaa taacctcgac gttattgacc tgatgcccct cgtcggtaag gcgctggata
    12481 ccacgcagcg cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga
    12541 gacatggcaa ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc
    12601 cgttgtcacc tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt
    12661 ttgccttttt gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc
    12721 cgtggaacgc gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag
    12781 ccggtgcagc ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg
    12841 gtggtaatcc gaatgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt
    12901 caacctccgg acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg
    12961 agctgaaaaa tccagagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa
    13021 ccggcccgct ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat
    13081 acgctaacgg ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca
    13141 gcaaaatcaa cgatttggat gctgacgagt tcctcgatca cgtagcgcga aatcaccctg
    13201 cgccgcaggc tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca
    13261 gtcgcgcact ttgtccgctt tttcccgggc ttctcgctcg agagagaaaa taatccgctg
    13321 taattgccgc tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt
    13381 gatggtttca ttcttgccat caatcacttt gcgctcgctg atggagatta actgggcgtc
    13441 aaagtgaaaa acaccccatt gcctcatgtt gacttcaatc tgagcgaagc gcatgccttc
    13501 ttgtaattcg gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc
    13561 atacaggcgg aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg
    13621 atggagtggg gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg
    13681 cggtacggtg ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt
    13741 aaactcgact ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac
    13801 ggcgatgttg tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc
    13861 cagtattttg ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt
    13921 gtgcaaatcg cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg
    13981 gtaatgactc acgcggaaaa actcctgact gataaactgt ctttggttta tcggtcacag
    14041 ttaacaaaac ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga
    14101 catacgccca gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct
    14161 tgcgtccgat tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg
    14221 gcctgatagc cggcattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt
    14281 tcctgacctg tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt
    14341 ttggcattgg cggctccatc agtggtttta atctgcacag cttcctggtg gcggtggtgg
    14401 gagctattct cgttctgggc atattccgcc tcctgcgaag agaataagat tttcataagg
    14461 cggatagcga tacagatgcc gctatccgct ttcacatcag aacgtatact cgacacctgc
    14521 tttacgggtg aaaaaaatca atatagcact tcgatatccg ataaagtcgg ttcccggtaa
    14581 caacaacaga atcctttttt aattattgtt tcgttgtttt tggtgtgatt cagaaaataa
    14641 atattttaga cagtagccag tcaggcgtta aaaatggggc ggatagtgag gcggttttgg
    14701 ctgcgcttat tgaacattta gaattgatca atccttcttg cagattaagc caaaataccc
    14761 gttcggcaat gcttcagtta cgcgaagagg aatggtctga attctttttt tggttgctaa
    14821 attctctgga gtgtctggat tatgtgatta ttaatttaac tccagaaagc aaaaaaacgt
    14881 tgatgagtga acaccgtaac aatattcagg tagcaatcga tgctttatat aggcaacgtc
    14941 ggtataaatc accgggggat gatagtgaaa cacttacccg tagaaatgac gctatcttcg
    15001 gcaaccacgt atggcaaaca tttgcgcaat acttcccgcc aggacttgaa aaaccgtctg
    15061 tgtagaaaac accatagcta tggtgttatc tgccgacaac gccatccgga gaagccgaat
    15121 gacgttgtcg gagtaagcgc tggttaaggt gtgggttgtg cctctttggt tgagggttgc
    15181 gtcgttgctg acttaacggt cggacgcgtc gccggaacat tgtcacacgg ttgctctttc
    15241 gggcagatca aatccagcat tttcagcgtc acgccattgg tccagccaaa gccatcctgt
    15301 aatggatatt cgccaccgcc gccccccgtt ccggtggtgc tgacatcata tttttccacc
    15361 agctttttct cccggtcata ggtgtgctga acattggtca ggaagtgcca gctaatgtcc
    15421 atcgccacct ctttttgccc gtagttttgt aatccttctg tcgcgaccca ctgtaacggt
    15481 gcccagccat ttggcgcatc ccattgttgc ccacttttca ccgacgtggt gttcaggccg
    15541 ccgggttgca gcagatgtgt tttcgtcgcc gtcgccattt tgttggcgcg atctttcgct
    15601 gccgcattga cgtacagcgg gaacagggcg gccgcggtta actgattgcg cactttatga
    15661 cttttcaggt cgtaatcggc ataccagcct tgttgatcgt tccacaggta tttttcgatc
    15721 cctttttgac gggcatttgc cagcgtttcg tactggtttg ccatcgcgtt atctccggca
    15781 gctttgctgg cgcgggcgag gattttttcc attttaaaca tcaggctgtt cagatcgacc
    15841 ggtacgatgc tggtggtgcg taaggtattt aactgctgcg ggttgtccat ccagcgcgag
    15901 ctgaaatccc agccagacgc agcggcagag cgcaggtcgc ggtaaatttc agtggcaggt
    15961 cgattcggat tgcttttggc ggtggcaata tcttccaccc atgactctgg tcgtggcgta
    16021 tcgcgatcgt cccagtagcg gttgagaagg gtaccatcct gaagtttgac aacgcgtttt
    16081 tcctgttgtc cggcttgcag gttttcaaca ccgtccatcc agtaagcata ttctttttgc
    16141 atttgcggca ggtattgctt caacgcggca tcgccttcat gctgcgccag taactctacc
    16201 atcatggcaa agaagggcgg ttgcgagcgg cttaaatagt aactgcggtt gccgttggga
    16261 atatgaccgt aagtgtctat ttcatgagca aaattggcca ccatatccgc gactttatcc
    16321 cagtgaccgc tttcggcaag tcctaacatg gtgaagtaac tgtcccagta atatacctcg
    16381 cgaaaacgtc cgcccggcac gacataaggt tccggcagcg gtaacagaga atcccatttt
    16441 tctgtgtttt cggtagaacg cgttaatacc ggccaaagtc cgtcaatatg ttcgcgcagt
    16501 gactgcccct ctggcggaac atatttctcg ccttctttcg gcagggtgaa attgacgtta
    16561 acgaaatggc gcagatcaaa tccgctctgg ttttgctgca tccgataatc agcaaggatc
    16621 atcagcggat cgctgttcgg cacggcatcg gcaaaggttt tttggtccgg aaaaagtttg
    16681 gcgttttgca catcattaaa cagcgaccct aataaaatat caggcggctg tggtgttacc
    16741 ggtgtttctt ctgcctgcac cgatagcgca gcgaaacaca aaaagataca ggctggaatt
    16801 aacgccattt tttgcgggcg agaaggtgcg ggggatttca tcaatcattc tcctttggcg
    16861 aaaccgaata aaacggtatt caagtatcag aaaaccttag ttcaggatcg cactagctgc
    16921 atgatcgaaa tctcattctg cgaactgtca ggcattttag aaaagggaat taaagggata
    16981 aataacggcg aaatagagtg atcaaataac gtctgtttat tagccagcca gcggcgttgg
    17041 gttcgtgatt agcgaagaaa aatcatcata aattggtgaa aaatataaca ggtagttaaa
    17101 aaccattagt gctgagtaaa ttgccggatg acatcagaac gatgccatcc gaacagtggc
    17161 ttaaccctga cggttgaaac gttgcctttt aacgtccagc gttagcgttt cttctggttg
    17221 tatcgcatac agtttctcac cctgctggca aatccagcca atccccagtt cacgggcgat
    17281 tagggcgctg tgggataccg gacttccggc gctaaggcag atacctttta caaccgccgg
    17341 atccagttgc agtactgtgg aaggataaat gttctccgcc agtagaatag ttggcgagtt
    17401 aaactgcggg agttcttctt tcgtttgggt caggtggacc agggtgcgat gcagaagatc
    17461 gtccacatca atatagcgag cttgtagata ttcatcatcc agttgctggt attgctggct
    17521 aagttcttta agaacttgct gccaggcata ttctgccgtg caatgttcat gctgaaggag
    17581 ttcgcttgcc gccgccagca gttccggatc atctaacagt gtatggtgac cagaaaagat
    17641 tgcggcaata tcgtcaagcc cgctggcttc tgctttcgct gttaacgtca tcagatctaa
    17701 taacgtgaag tcaatagcct ggcgtaatcg atcttgttct tcttccacgg tcagggttga
    17761 ttttgcctgt accgtacata aaactggttg ataataaaag gctttacccg aaacaggcgg
    17821 aacgggacgc agagtaggtg gagcgacttc ctccgtttca ccaaagttat cttcagccag
    17881 ctgacggaaa gcgatcagtg cctcttcagc ttctggccct ttcgcaatca ggcgcagcgt
    17941 atcgttatag cgaacttgta gtaacgcaat ctggttaata ctctctggtg tgacgcattt
    18001 gccgtttttt tccagcaaca tatcggcatt aaatgtcgat aaggtataaa ccagccggga
    18061 ggccggacgt acatgcaggc cgttacggtt ttttatgacc accgccagag aacgggcttc
    18121 ttcatcgtac gcaggacatg tgtcagagat tttagtgtcg gaggacggta aacccagttg
    18181 ttcacgtttg gcttccagcg catgcatagc gtcaaagata actttgtcga tatccgctcc
    18241 cgaggccgcg ctgaccgttg ctgccagtgt accttcgacc aacggcgcag cacacaaacg
    18301 tacttttgcg gcgatcttgg gagccagcaa ttccagcgca gtttcagcac tcaataatgc
    18361 gctacccata tccatcatga ccagcacatg gtcggcatca gcaacagatt cgatggcctc
    18421 catcactttg acggcatcgg taccaatagg attttgtgga tcgtcaattc ccgcggcaat
    18481 ggcgatttta caactatcac tcattaacat ctgacgggct aattcaccga caccttctcc
    18541 cagtcggctg ctatgtgaaa ctatgaccag gtttaccatc accaattcct tactcttttg
    18601 cggctaacgc caacatttgc atcataaaca tcaccgaggt cgcgccggga tcctggtgac
    18661 caatactgcg ttcaccgaga taactggcgc ggcctttgcg ggcttgcatc gtaatcgtac
    18721 tttgtgcagc ggattcggcg attctactgg cagcttcgag cgccaccgga acagagagat
    18781 tttgctcgct ggactgacgt aacgattcca ccaccggcac ccacacatca cacatggttt
    18841 tatcgccagg ttcggctttc ccgcgactga ttacgccgtc cgcgccatcg cggaacatct
    18901 gataaagctc ttccagtgtc aggctttgcc gtgcctgggt cgcctgtgcg gcgcggataa
    18961 agaaggtacc gaacagcgga ccactggcac caccgacgct ggaaagcagc gtcataccgg
    19021 tattcttgag aatgaaaccg atatctttat ctgcgatagc agggagtttt tccaccactt
    19081 tgctaaagcc tcggttcata tttagcccgt ggtcagcgtc gccaatttcg cgatccagtc
    19141 cggtaagata ctcgctctcg gtgctgaaaa tatcgccaca acgagtgagc cagttaacaa
    19201 tttgagttct gctcagtgac attgctttct ccttatttac cccagttaag ggccggggtg
    19261 tggaccgggg cgtcccagag tgccagcgtt tcgtcatcaa ctttcagtaa ggtgattgag
    19321 aaaccggtca tatccagtga ggtgcagtac gcgccaatta aattacgttc gatagtcaat
    19381 cccgcttgct ggcaacgtgt ggtcaggcgg ttatagacgc cgtacagctc agaaagcgga
    19441 gttgcgccaa gattgttaac cagcgcaatc acccgatcgc cagactggag cggttgtttg
    19501 gtttgttgtt cttcctgcca actgccttgt tgataatccc agaaacgcaa agtgcgatgg
    19561 tatgagccat ttaccagcag ggtgtcgaac atttcatcga cggtttgatc aagggaagag
    19621 aaggggcggc ggtcaatacc cggctcacca tgaatgccga cgccaaactc catctcatta
    19681 tccgccaggg taaaagaagg tttgcccgcg gcaggaacgg tacaggcacc gagagcgata
    19741 cctattgagt ggccttgatt attcaactta cgccccagtt ccgcacaggc gtccagtgag
    19801 tcgccacgct ccgccgctgc gcctacgagt ttttcaatta atacggtgtt ggcaacgccg
    19861 cgtcgcccgg cagtataaag actgtctttt accgcaacgt cgtcatcaat gaccacagtg
    19921 gtcactttta cgccgctatc gtgcagtaac tcggtcgctg tttcaaagtt aagaatatcg
    19981 ccggtgtaat ttttgataat catcagtaca ccttcgccgc catcaatttg catggcgcat
    20041 tcaaagattt tatcgggcgt cggtgaggtg aaaatttcgc ccggacaggc ccccgaaagc
    20101 atcccctgac cgatataacc acagtgcatc ggctcgtgtc cgctgccgcc acccgacagc
    20161 agggcgactt ttcctgcaac gggggcatca gctcgggtga catacaccgg atcctgatgc
    20221 agtgtcagag atggatgcgc tttcgccagt cctgccagtt gttcgtccag tacgtcttgc
    20281 acatcattga tcaatttttt cattattttg ctccagcaat tacggtaggg catggatgat
    20341 gttcaacgac acggcgacca ctgactgccg atgaatccat tgtgcatcag gcaagggaaa
    20401 agaaaattag cccaaattat gtttcatagt gaaacatatg ctttaatgaa tgttccatat
    20461 tgaaactttt acgtgtatta atacttaaaa ttgcgagccg gaacaccttt tgtcataagg
    20521 gatgcgggat atgagtggcg cttttaacaa cgatggtcgg ggcatatctc ccttaattgc
    20581 aacctcctgg gagcgatgca ataagctgat gaaacgggag acatggaacg taccacatca
    20641 ggcccagggc gtgacatttg cttctattta tcggcgtaag aaagcgatgc tgacgctcgg
    20701 tcaggctgcg ctggaagatg cctgggaata tatggcaccg cgagagtgtg cgctgcttat
    20761 cctcgatgaa accgcctgca ttctcagccg taatggcgat ccgcaaacct tgcagcagct
    20821 aagtgcactg ggattcaatg acggcacgta ttgcgccgag ggaattattg gtacttgtgc
    20881 gctatcgtta gcggctatct ctggtcaggc cgtgaaaacg atggccgatc aacatttcaa
    20941 acaggcactc tggaactggg ccttttgtgc aacgccgttg tttgacagca agggccgatt
    21001 gacgggaaca atagcgctgg cgtgtccggt tgagcaaact accgcagctg atttgccgtt
    21061 gacgttggca atcgcccgcg aggtcggtaa tttactgctg acggacagtt tgctcgctga
    21121 aactaaccgt catttaaatc aacttaatgc cctgttagaa agtatggatg atggcgtgat
    21181 tagctgggac gagcagggta atttgcaatt tattaatgcc caggcggcgc gggtcttgcg
    21241 ccttgacgcg acggcaagtc agggacgggc aatcactgaa ctcttaacgt tacccgccgt
    21301 attgcaacaa gcaataaaac aggcacatcc gctcaaacac gtagaagcaa cctttgaaag
    21361 ccagcaccag tttattgatg cggtgataac ccttaaaccg ataatagaaa cgcagggaac
    21421 cagctttatt ttgttgctcc atcctgtgga acagatgcgg cagttgatga ccagtcaatt
    21481 aggaaaagtc agccatacct ttgctcatat gccacaggac gatccgcaaa cccgccgctt
    21541 gattcatttt ggtcgccagg cggcgcgcag tagctttcct gtcctgcttt gtggagaaga
    21601 gggcgtgggc aaggcactgc taagtcaggc aattcataat gaaagcgagc gtgctgcagg
    21661 tccttatatc gccgtcaatt gtgagttata tggtgatgct gcgctggcgg aagaatttat
    21721 tggtggcgat cgcacggaca atgaaaatgg ccgtctgagt cggctggaac tggcgcacgg
    21781 cggcacgctg tttcttgaaa agattgaata tctggcggtg gagttacagt ctgctttgct
    21841 tcaggttatc aagcagggtg ttatcacgcg actggatgcg cggcgtttaa taccagttga
    21901 tgtcaaagtg attgccacaa cgaccgcgga cctcgcaatg ctggtggaac aaaatcgttt
    21961 tagtcgccag ctgtattacg cgttgcatgc atttgaaatt accatcccgc ctctgcgtat
    22021 gcggcgtggc agcattccgg cgctggtgaa taacaaatta cgcagtcttg aaaaacgctt
    22081 ctctacgcgg ctgaaaattg atgacgatgc cctcgctcgc ctggtttcct gtgcatggcc
    22141 tggcaacgat tttgaacttt acagcgtcat cgagaatctt gctctgagta gtgataatgg
    22201 gcgcattcgc gtcagtgatt tgccggaaca tctgtttacc gagcaggcaa cagatgatgt
    22261 cagcgccaca cgcctttcca ccagtctgtc atttgcggaa gttgaaaaag aggcaattat
    22321 taacgcagcc caggtcacag gcggtcgcat tcaggaaatg tcggctttac ttgggatcgg
    22381 ccgcactacg ctgtggcgga aaatgaagca acatggcatt gatgcagggc agtttaagcg
    22441 ccgggtatga aagacagaga cgatttctga tacatcagag tgatctatat ttcattccgg
    22501 cgcacgctaa catttttcag catcgtttaa gggctggtct atcccgcact taaaagctga
    22561 agcgatatcc tccgttgact tgcttctgat caaagaggtt actctgcgtg taatccgctt
    22621 cgaggtagaa tgtgtgctgt ttgttatact gtgcactgac tcccacgccg ttattccagc
    22681 cattaccttt gaaactgtac ttctcccggg agttgttcaa cagatagtct gtatcccctg
    22741 aaaactcgcg gatagctcca gtcttcacat acatattcag ctggctgttg cctgcggtga
    22801 tgtcatatcc cagtatcatg ctggcacgcc ccagcagcga ttcgtagtga ttcagatgta
    22861 tattgagacc attactcgcc ttcatagcca tctcattctg gtggctgtat gtgagctggg
    22921 tttgcggctc tatatagaac ccataaccag taggggtcag gttgaacctc tgcccggctt
    22981 ccagggagat gctcaatcca ttcgcagtgc cgttggcttt aacgccgttg ttctgactgt
    23041 ccagtacgtg aaaactattt ttctggcgcg atgcttttac aacgagatcg ctgtaaaaac
    23101 cgttgtgtgc catgtaactg gcatacattc ccatgtagtc tgaacgtgcg gtaccgtcgc
    23161 ctccgctata gtccggcgat gcatgtgttg agccaatata cagaccgaca tacaacggca
    23221 ttacatcaga gagacgttta tccccaccaa actggatacc gctgtaaccc atgtcaaagc
    23281 cgctcagttt gccactggca aaggagtcca ggcttccccc ataactgcgc aaccagatat
    23341 taccgtcttt actctgattt cgcaggtcac ccatccgttg catcagcgta cggttttcaa
    23401 catagttcaa taagtaaccg acattgagat aattgccgcc agcatctgcg gtcgtggtgg
    23461 gtttaggcgt gggagcgggt tcaggcgtag gatcggggtt gactatggga ggctgagcgg
    23521 gagctggtgt gggttcagga ttaggagtcg gttccggaac tgtcccggaa gcgtaaagct
    23581 cccagttagt gccattttta cgcacatcgt acagatatcc ccccaactca acctgagaag
    23641 acgcgctgaa cgaggccgcg ccgtcagtgg tttttaccac tgtcagaact tcatttcccg
    23701 ttgtggcctc gctgccctgg ttgcggatag ccaatacgtg attaccagca ctactcccgc
    23761 tgatattcaa taaatcccct ttattattaa cgccattacc ctcgccaaca acatcagcac
    23821 gcataataaa ggtactgtta ccgctcaggt tctctacgtt taatgtggca aatgtgccgg
    23881 cagttgaccc gtggctggca aaatcgacag ttgaatggct cagcgccagc gtgtcgagat
    23941 tagaattact tgttacgttc cagacgctgt tattcattgc aacgtccagt tttccgccat
    24001 tgacattatc gctgagggag gaacctgtcc aaaccgaccc agggtgcata tccagattga
    24061 ttagcccacc tttggaaaga acgctaccgt tgataagcat acgacccgag gcgttgatgc
    24121 ggctggcggc ataaccatcg tcatgttgcg ttgcaatggc catctggtcg ggtgtactca
    24181 tatcaatgac cagatcgctc gtgaggtcga tctggctgtt ggtcatggca taaattcccc
    24241 tggctcctgc cgcgccggtg atagccaaac tgtctccggt tattctaccg ccgctgagcg
    24301 cccacaaacc cagcgccaga ctgccattac gatcaactgt aatatcggta ttttgcatgt
    24361 tgacaacagc cgttgccgtc tgggccgagg caccataaga accgccggaa aagatgctgt
    24421 ttcgttgcgc cgccgtgcca gaaaaattga ttgtcgcgtc tgaaccactg gtgacgaggc
    24481 caccgccctg cgcggaagaa atatggctat ctgcaccgat agtggttgta ccaccacgaa
    24541 cttcgacgcc attggccgcg gctccagtta catcaacagt gagtgcattc gcgctaacct
    24601 ggccaaagct ccagaggccg tgtgcattat cgccattggt tttaatggaa ctgtttgttc
    24661 cgagatcgac aacagagttt ttctgtacgt ttatccccat ggcgctgtag ccctgaacat
    24721 cgattgtcag gtctgtagcc gtaaaacgcg cagcaccatt ggcgttattg ccgttgagac
    24781 caccgatata aacacctgta cttccatcgg tcgtgatttt acttccgctt ccaagatcga
    24841 cactggtgcc atagtcattg atggttaggc ctataccgtt cgagttttca atggtgaatt
    24901 gagtggctgt tagtgttgag ctatgcccaa taatgatgcc gtcatcgtta ctcttaatgg
    24961 tgctgcctgt gcctaagtca gcatgggtat agtcaccaat taagttaatg ccgatggcag
    25021 aggtttgacc aacaacatct actgtgagtc ggttagctgt cagggagctg tttttgccct
    25081 gaataatgat tccttttgca tatccagagg cgtcgttgac attgacggtg acatcattac
    25141 ccaggttgat attgccaggc gtgtttcctg ctggggtcat tacaccatag aggtttcctg
    25201 atggatcggc ggtatctcct gtaattctgt caccatcatt aatagtaata ttggtgccat
    25261 attgacttcc tgtatcagca gctatcagca ggggggaaaa gataattgca ttcaaaccaa
    25321 tagcgccaaa ttttatcagt tgaattgagg gcgaacggat ttttaattca gcgagacgat
    25381 ctgctttggt attcccattg tgttgtttga tgcccatacc actcctatat agtacccaga
    25441 ctatgggtct atggattttg tcattttgtt taaatcaatg aataatatcc tcttatcatc
    25501 atgatatttg tatggtgcaa tcatgatgag cttctcagag aataattctc tattaccggg
    25561 caaaatcagg aacacaggaa ttaatatata tttaacatcc cccttacaaa ggtgggttta
    25621 tatcgagtta ttccctaagt tg
//
LOCUS       DAZFVO020000062        25562 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFVO020000062 DAZFVO020000000
VERSION     DAZFVO020000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.062"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..204
                     /locus_tag="ACHKNB_RS11845"
                     /old_locus_tag="pgaptmp_002369"
     CDS             <1..204
                     /locus_tag="ACHKNB_RS11845"
                     /old_locus_tag="pgaptmp_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4195868.1"
                     /translation="YLAYMEANGYRNVLSLKMFGLGLPVMLKEYGLNYEKRHTKQGIQ
                     TNLTLKEESYGDWLPKCDDPATA"
     gene            409..744
                     /locus_tag="ACHKNB_RS11850"
                     /old_locus_tag="pgaptmp_002370"
     CDS             409..744
                     /locus_tag="ACHKNB_RS11850"
                     /old_locus_tag="pgaptmp_002370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195869.1"
                     /translation="MSHIGRMPEVKNRMFTLHPLFTTYHSEIKGENRKVNSVNSSFEK
                     KFFMWIKDALVWIRATDPNLLIKNDLTFRFIDGLKIDWSEKQWGHKRGHISLLCFIIC
                     FLSLTYFDV"
     gene            960..2225
                     /locus_tag="ACHKNB_RS11855"
                     /old_locus_tag="pgaptmp_002371"
     CDS             960..2225
                     /locus_tag="ACHKNB_RS11855"
                     /old_locus_tag="pgaptmp_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4195870.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(2426..3895)
                     /locus_tag="ACHKNB_RS11860"
                     /old_locus_tag="pgaptmp_002372"
     CDS             complement(2426..3895)
                     /locus_tag="ACHKNB_RS11860"
                     /old_locus_tag="pgaptmp_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HGU4195871.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(3892..4434)
                     /locus_tag="ACHKNB_RS11865"
                     /old_locus_tag="pgaptmp_002373"
     CDS             complement(3892..4434)
                     /locus_tag="ACHKNB_RS11865"
                     /old_locus_tag="pgaptmp_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195872.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            complement(4442..6127)
                     /locus_tag="ACHKNB_RS11870"
                     /old_locus_tag="pgaptmp_002374"
     CDS             complement(4442..6127)
                     /locus_tag="ACHKNB_RS11870"
                     /old_locus_tag="pgaptmp_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HGU4195873.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            complement(6117..7274)
                     /locus_tag="ACHKNB_RS11875"
                     /old_locus_tag="pgaptmp_002375"
     CDS             complement(6117..7274)
                     /locus_tag="ACHKNB_RS11875"
                     /old_locus_tag="pgaptmp_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195874.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            complement(7277..8125)
                     /locus_tag="ACHKNB_RS11880"
                     /old_locus_tag="pgaptmp_002376"
     CDS             complement(7277..8125)
                     /locus_tag="ACHKNB_RS11880"
                     /old_locus_tag="pgaptmp_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195875.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            complement(8136..12422)
                     /locus_tag="ACHKNB_RS11885"
                     /old_locus_tag="pgaptmp_002377"
     CDS             complement(8136..12422)
                     /locus_tag="ACHKNB_RS11885"
                     /old_locus_tag="pgaptmp_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195876.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            complement(12419..13705)
                     /locus_tag="ACHKNB_RS11890"
                     /old_locus_tag="pgaptmp_002378"
     CDS             complement(12419..13705)
                     /locus_tag="ACHKNB_RS11890"
                     /old_locus_tag="pgaptmp_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195877.1"
                     /translation="MDSITTRQNNDPVNSEAALNLCLQLWHQGGLTASKAALLLAAVP
                     ALRSLLQPIIQPKKKDAETDIISAFSLTAPLLDAFSDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMSDPMVLVKLIQQLGNASEWVLAQLENADFSPQIIASPLAQTER
                     DLLGHSLNDNAAIPALCRILRTSHTLFTVSEQNEPPASIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHNLKPNADWLLVSRSGHDNVPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGESGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRAIKQLAQNCYANKKRAANRINLQGSSL"
     gene            complement(13723..15249)
                     /locus_tag="ACHKNB_RS11895"
                     /old_locus_tag="pgaptmp_002379"
     CDS             complement(13723..15249)
                     /locus_tag="ACHKNB_RS11895"
                     /old_locus_tag="pgaptmp_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4195878.1"
                     /translation="MDKFFRTNSSILVERLDPNFYQPSLVENDKLIKSKGWVELQSLY
                     EASGIGNTSAVERYYSEAADSIPYISGKVIKSFNIDLDECQRISLDSHKNELTKSALK
                     PTDVLVIRKGDMGNACVVPSEVNEANCSSEVIYLKMKASSDPYYLVSYLNCDQGQKAF
                     KRLGRGTIIPGVSLLDVPRLPIPKVSEFVQKYIGDKVRQAEQLRAWAKLLRTSVDAHL
                     NSLNLPINEPPALLNRVSAQTMEDRLDPRPYRTHYLCLVREIEKLPHDSISTLVELAS
                     GCPVSSNDFLENSGIPLVRIRNIGFDDFIGLDTGVSQDVYQDATKYQAKDKMIVVGMD
                     GIFRSQFFISDELPMLVNQRVAMLSPQNIRGELLTHWLNRPEGQMQLNQWAVKTTVEH
                     TSLSDIGRVLIPRLDKSLENKLADYLLNARLAYRYAKFLTQVAKTLVEALIEGQLTEQ
                     QLIQAQQALEDGDNSFDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
                     "
     gene            complement(15249..17396)
                     /locus_tag="ACHKNB_RS11900"
                     /old_locus_tag="pgaptmp_002380"
     CDS             complement(15249..17396)
                     /locus_tag="ACHKNB_RS11900"
                     /old_locus_tag="pgaptmp_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4195879.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            complement(17447..17833)
                     /locus_tag="ACHKNB_RS11905"
                     /old_locus_tag="pgaptmp_002381"
     CDS             complement(17447..17833)
                     /locus_tag="ACHKNB_RS11905"
                     /old_locus_tag="pgaptmp_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195880.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            complement(17830..19173)
                     /locus_tag="ACHKNB_RS11910"
                     /old_locus_tag="pgaptmp_002382"
     CDS             complement(17830..19173)
                     /locus_tag="ACHKNB_RS11910"
                     /old_locus_tag="pgaptmp_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGU4195881.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAAIYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPFRIMAKRGERMMWPNDVSCTMSPQLDRMAASVSYSGGMR"
     gene            complement(19166..21241)
                     /locus_tag="ACHKNB_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /pseudo
     CDS             complement(19166..21241)
                     /locus_tag="ACHKNB_RS11915"
                     /old_locus_tag="pgaptmp_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
     gene            complement(21411..22283)
                     /locus_tag="ACHKNB_RS11920"
                     /old_locus_tag="pgaptmp_002384"
     CDS             complement(21411..22283)
                     /locus_tag="ACHKNB_RS11920"
                     /old_locus_tag="pgaptmp_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HGU4195882.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            22383..22487
                     /locus_tag="ACHKNB_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /pseudo
     CDS             22383..22487
                     /locus_tag="ACHKNB_RS11925"
                     /old_locus_tag="pgaptmp_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(22495..23475)
                     /locus_tag="ACHKNB_RS11930"
                     /old_locus_tag="pgaptmp_002386"
     CDS             complement(22495..23475)
                     /locus_tag="ACHKNB_RS11930"
                     /old_locus_tag="pgaptmp_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4195883.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(23540..24646)
                     /gene="nanM"
                     /locus_tag="ACHKNB_RS11935"
                     /old_locus_tag="pgaptmp_002387"
     CDS             complement(23540..24646)
                     /gene="nanM"
                     /locus_tag="ACHKNB_RS11935"
                     /old_locus_tag="pgaptmp_002387"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4195884.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(24666..25382)
                     /gene="nanC"
                     /locus_tag="ACHKNB_RS11940"
                     /old_locus_tag="pgaptmp_002388"
     CDS             complement(24666..25382)
                     /gene="nanC"
                     /locus_tag="ACHKNB_RS11940"
                     /old_locus_tag="pgaptmp_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HGU4195885.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
BASE COUNT         6541 a         6058 c         5738 g         7225 t
ORIGIN      
        1 tatctggcct acatggaggc aaacggctac cggaatgtac tcagcctgaa aatgttcggg
       61 ctggggctgc cggtgatgct gaaagaatac gggctgaatt acgagaagcg ccataccaaa
      121 caggggatac agaccaacct gacgctgaaa gaggaaagct acggcgactg gctgccaaaa
      181 tgtgacgacc ctgcaacagc ctgacccacc tgaccggcat ctgccggtct ttttttatcc
      241 ctgaattccc cgaaggtgaa caatccactg ttcacccttc accgtatatt cacccgttat
      301 cacactgaaa ttaaaagaga aaaacgaaag gtgaacagtg tgaacaatca aatcaaaaaa
      361 aacttttttc ttcctgagtg atttcagtgc ggaggattaa tcaccggtat gagtcacatc
      421 ggcagaatgc cggaggtgaa gaatcgaatg ttcacccttc acccattatt caccacctat
      481 cactctgaaa taaaaggaga aaacagaaag gtgaacagtg tgaacagttc tttcgaaaaa
      541 aaatttttta tgtggataaa agatgcactg gtttggatcc gagctacgga tccaaactta
      601 ctgattaaaa atgatttaac tttccgattt attgatgggc tgaaaataga ttggtcagaa
      661 aaacagtggg ggcacaaaag ggggcatatt tcattgttgt gttttattat ttgtttttta
      721 tcattaactt attttgatgt ttgagtccgg ccttcgcacc aaaagtatgt aaatagacct
      781 caactgaggt ctttttttat gcctgaaatc cagtatccgc aaggctttcc tcgctttttc
      841 actcaaccta agtcaacctg attcaatcta catcaactta ctgatgcggg tacaactgcg
      901 ggtatttccc ggttcgatta tgtttgtacc cacacagggc ccttgaaagg atactcatca
      961 tggctctgag tgatgtgaag gtccgttcgg ctaagccgga agcaaaagcc tataaactta
     1021 ctgacggtga aggcatggtt ttgttggttc accctaatgg ctccaaatac tggcgacttc
     1081 gttatcgttt tggcggtaag gagaagatgc tggcgctggg gaaataccct gaagtctcgc
     1141 tggtcgatgc cagaggtcgc cgggatgaag cccgtaagtt gttagctaac ggcgtcgatc
     1201 ctagtgagaa caagaaagct gttaaggtag agcatgagca agaggtgata acatttgaag
     1261 tggtagccag agagtggcac gccagcaatc agaagtggtc tgcatcgcat agcgctcgtg
     1321 tattgaaaag cctcgaagat aatctctttg ctgctatcgg taagcggaac attgctgatc
     1381 tgaagactcg ggatctgctt gtccccatca aagcggtgga gtcgtctggg cgtctcgaag
     1441 tcgctgctcg tttgcagcag catactaccg cgattatacg ctttgctgta cagagtggat
     1501 taatcgacta caaccccgct caagagattg ctggtgcggt tgctacggcg aaaagacagc
     1561 atcgtgctgc tctggaactt aaccgcattc ctgaattgct tcaacgcatc tatcactatt
     1621 ctggcaggcc gttaacccga ctggcggttg aactcacatt gttagttttc atccgttcaa
     1681 gcgagttgcg ttttgctcgc tggtcagaag ttgattttga tactgccatg tggacgattc
     1741 cgggagaacg tgagccactg gagggagtta agcattctca gcgtggttcg aagatgcgga
     1801 ctccacatct tgtaccctta tctcgacaag ctctcaccat tctggaaaaa atcaaaagca
     1861 tgagtggaaa ccgtgagctg atcttcgttg gtgatcacga tccgcgtaag ccaatgagtg
     1921 agaatacgct taacaaggcc ttgcgagtga tgggctatga cacgaaaacg gaagtgtgtg
     1981 gacatggttt caggacgatg gcgtgtagtt ctttgattga atcgggattg tggtcgaggg
     2041 atgcagtaga gcggcagatg agtcatcagg aacgtagctc agtacgggcg gcttatatcc
     2101 ataaggcgga acatcttggg gagcgaatgt tgatgctgca gtggtgggct gattatctgg
     2161 atgcgaatag aatggcggct atcactccat ttgactatgc aaagattaat agttcttttc
     2221 gataattcta gctaagtata tgaatattaa ctttttttaa ttaaaggctt ataactgaga
     2281 agcctccgcc ctaatgggga gaggcttttg tcttaactca atcaagaagg aaatcgttat
     2341 gtaaaacttt atttttttgt ccggtatgtg aacggaagtg ttaaatatcc actatgcctt
     2401 gttcgcgcaa tggtcatcct ttcaatcaaa taactccaag ctttgcatac aattcttttt
     2461 tctctttcat gctgttatcc aatggtgcta ctcgcaatgc cgctttcaaa taagccgctg
     2521 caccagagta atcacgcatt tgctcgcaaa ctaaaatgag ttggcttaac acatggaact
     2581 tcattggagt tgtttggttt actttctcca atagtactct tgcttcagaa tatttcgctt
     2641 caaggccaag gatagatgat tctaaataca tggtggccgc gttttctgct tcagcttgct
     2701 ttcgttgcaa cagccagaaa tttgcttgac tgatattgcg taactgtagg tttgccttaa
     2761 ctatcaactc gaacaaagct gctggttgag tcaatttacc ctcaagtaag atcgctttat
     2821 tatcttctac aactttttca aagtcgaata agcgattggc gcgatacaag gcaataagtt
     2881 gctgatgtaa agcttgtaga tctttactgc ggctatactc ctgcgacgct aattgagcag
     2941 ctttttggta agattgatcg gacattgctt caagtaccgc acttgaccag tcctgagcag
     3001 gtttagtttg aaaaacggga gcaatagcac caacttttaa ttgcttagtg gcagcagaat
     3061 caaatgcatg aattaagtcg gaaatagtgg aaaagcgatc ctccgctcgt ttttgtaaac
     3121 aagtaagaac cacttgttcc aggtccgtat gaatagagct attaatctct gtcggaagct
     3181 tgaaaggtgc gtggcgctga acttgtgctg gcgtatcatg gggcagcttt cctgtcagca
     3241 gctggtatag caaaacacct aagctataga tgtcgcccaa tagtagctct tccaggctgc
     3301 ctctacggct atcgacgttg gagaactctg gggccgaata cagcacagtc ccgccatttt
     3361 cacgggtacg aacgtaaatt tcttctggta aagctgagct gccaaagtcc gtcaacttca
     3421 ccaggtcccg ctctccaacc aaaatgttat gtggcttaat atctccatga gatattggta
     3481 ctggtaattc ggccaaataa tgcacagcac agagcacttg acgatatatt ctgaacaacc
     3541 gttcataggt ctggccaaaa ttacgctgcc tgtcatcgag cttttgcgcc agggtttggc
     3601 tgggaaaata ctccatttca atatagaacc attcattatc tatacggccc atatcaaaaa
     3661 tttgcacaat attttcgtgc ggctcgacgt ttaaaagctc tttaccttcg gcaagctgac
     3721 ggtcttcccg agtaatatcc ttagggattt ttactgcgac aatttttcca tctttcaggc
     3781 gttccgcccg ccatacccag ccatggctgc catcaccaag gcattcaagt aacaggtatt
     3841 tgtcaccaag gcgtttattc gtccctggca aaaaatgtgc tgattttata ttcacatcaa
     3901 cacctcatag tgaattctgt ccaaaacaat catgttcagg tttttgataa ataaaaagcg
     3961 actaaatacc acctcaacat caggagcggc gattacctgg gtacccgtgg acgaagtaaa
     4021 acccatacgg catatgtctt tcagtttttg agtatcaggt acacctacta agccctggct
     4081 ccaggcactg aggaccaggg ttttggggca atgattccag cgctgtgctg cttccagtaa
     4141 gttttttaat ttttcttcca gcttttgctg ccccttcgct gaaccatcaa agcgttcaaa
     4201 ttcgattaaa cacacaggcg atgcagaaga tttatgaaac cagcccgagt cagagcgaat
     4261 atcagcacca aatgtccctg gagccgggca ttccactacc gccataaatg actgttcatg
     4321 cccgaatgta ttccaaacgc tacagcccag gctgtggacc aatacatcct ggtccaatag
     4381 cttaccgctt gctaaaagtg gaaatgtctc atttacccag gcaacttcag gcataggcac
     4441 cttagccact gaaagagccc caaacacgca gtacctctgg tgattcacca ccgtgaatta
     4501 atgccttact accaacttta acaagcagtg gtgcagggat tgaacgacca cacaccaaag
     4561 cttcaccggt tgataaactc gggagttcct ccagatcagc tttgctcacc atatctgatg
     4621 ctttagctat aaatcgttga tcgtcagggt tctttaagcg catcgtaatt agcgtattgc
     4681 actgagaggt cacatctgaa tctaatttcg atggacgctg gctcacaatg ccaaatccaa
     4741 caccaaattt acgaccttca ccagcgatct ttttaataat gcggtgactg actgcgttgc
     4801 cgccggccgg ggcaaaatta tgaccctctt cgtaaatgat aaagcatggg cgcagcgggt
     4861 ccgttttgct cgaggctgct tttaatattt cactagataa cagtgcacaa atgacttgct
     4921 tagcggtatc actcaaacct tgtaagtcaa caacgactaa acgcccctgt tgattgctcg
     4981 ggcgacccac cattttgtaa atatcagttg gctcagccat tgttgcgctg taaaaacttt
     5041 tagcttcatt aagcacacgt gaaagcttca tcgaggcgac ggcggcacta cggcctgata
     5101 acgccttcgc ctcagcttca gaaaggtcgt cccactcttt taactcttca ataccatcac
     5161 ctaataagaa gcgtaggcgg ttaatatctc gtggctcagt cttatctgca tagcgccaat
     5221 aacgaattgc aacatcgagt actcgttgct gcggttccgt taagccaggt aatatttcag
     5281 caatatcgtc catttcaaaa tggtcaaact gaagcgctaa tggcgtattc ttactggcgt
     5341 atttatgttt aaaagattca tgctgcggcg agtaaacctg aatacctgca ccggcggctt
     5401 gcagcttttc aaaggtctgt tttatcagtg gcagtgcttc ttgatcacgc ttatcgtcag
     5461 tggcttctaa gaagcttgag aattgcagat taccgccagc gagggcttta ccgtattcac
     5521 catgtgggtc aaatactact accgtaccgt tattaaccgc aactaaacgc tcaataatgc
     5581 ggccaactgt ataagactta cctgaacccg tcattgccag aacagccaaa tgctcagtga
     5641 ccaacttgtt cacgtcaata aaaacaggaa ctgtattctc acctttgtcg tagcctacta
     5701 agttacctaa gtgcaaactg gagtgttcat taaattcgta aaaatcactc aaaaactgaa
     5761 aatcgacggt ttcaacagcg gtacccgggt taagcgggcg acgtggaatt ttgatttgac
     5821 gagtaacagg atcgcggtaa ccaaccagct cgacctgagc aaacatattc tcaccagtta
     5881 cattcgcacc gggcaaaagc tcaagctcgg ttacaccatc gccaaagccg gcgttataga
     5941 gcatattagt acggcttatc ttggtcactc gccccagtac ggcgacttgc ccctcatctt
     6001 tgcgttcctg gtgcataatg cgtacaaact caccacggcg tactgaaaac gagtcttgta
     6061 caaccatagt taactgactg gcatcaccag tgttaccgac taatttacct aaaactttat
     6121 aagccattat tcaatccctc atcgaggcct gccaaccacc ctgcctcaat atcattattc
     6181 tttagtgcct ggtgtaaacc ctggcgataa aacgtagcaa atttaggtaa aataccaagc
     6241 tgttgataac caagtaattg tggcaacggc attgcattac ctttgttttg catatccagc
     6301 accatcaggc gagaggcaac taaatcgaga tcagcacttt tccattctgg ttcgtcaccc
     6361 gctaattgca ccatttttat ctgtccacct cgggcactac ggaagtgaaa accaattaga
     6421 ccttgcttag cttcaatatt gggttcaatg cgcttttctt gttcagacaa acgaaatgcg
     6481 atggtgcgcg aatagttgcc taaaatgcca ttgataaatc gcaaatctcc cacgccaacc
     6541 aaactttcat ccagtccttc cagattttgc atcaactctt tagaaaaccc atcagatgct
     6601 aaaatttgtt tgcgtccttg ttcggtacgt aaatcttgtt tggcgatact tacaatggca
     6661 ctaaagcgct cagccaaaat gctcgcaagt actacaccat tttgattcca gggaaaaatt
     6721 ctggagcgat ggttttgcca aaaagtttct atcacttgtc tggttttttc gtactcttgt
     6781 ttatgacgga catttctctc cagcggagcc atatcccgtg ctaaaaacaa cggcgtatcc
     6841 agcaaaatta aatcgccagg ctcataacct aaaagttttt tctcaactaa ctgataagcc
     6901 tgacgaattt caattaaatc aagccgttta cgctgagctt caaaatcaat atcatccagt
     6961 tctgataccg agtcgctacc caccgtgctg actttattgt catctattaa caaatctgtg
     7021 cgcactgctg cactggcata aataaaacca cccaatacct tgtctaagca cgatttagtc
     7081 gcaataccgg ctacgcgtaa attaacaatt gtgggcttgg ggaccgcttt cactaagcca
     7141 gcatcacgga atagcggcgc cacttgctca atatcacgga cctgtttaag caagcgtcct
     7201 gcacttcccg gtaagcgaag ctggctatcg atggcgagcg attgcgctaa attcatcgtt
     7261 ttgttctcat tcatgcttat agcgttcctg tgtgtagctg ctcgccaagt aatggggcat
     7321 aagcccaata ttgcgaggct tgtaggcatg ttttaccggt agctgttatg cccgcgtcgt
     7381 cgataaaatc gtgctgccgc aacatctcac cccgcttgac aatatcaagg ctattaacat
     7441 caaacatcag cttaaccgca ttatgctcag acagcgcttg taagttcatg ccaccgccag
     7501 cgtagagtaa tagcaataca accagatcgt gcttatctgg cgtgtagtca gttgatggcg
     7561 ctttaggttc gattattcgt tcagcaatgc ctgcatggtc cacaaaccaa ttcaaccagc
     7621 tcaccaggag ctcactatga tcgagtaaag tcagcgacca caaaggtaac gtggtaaagg
     7681 ctaatagccc actgttgctg tgttttcgta tgtagcgggt atgcactaca tcaccggcct
     7741 ggtgctggta gcgatctgcg ctgccagtaa agccagcaat agcctggtct gtttcactgg
     7801 ctagcaaagc ggttagcccc ggttcctgcc ccctcaagct cgctggtact aactgccaat
     7861 ccagtggata gggcaattgc gctaataagg taaattgttc tgctttcagg tttattacca
     7921 aagcggcaca acctgcctgc atagcccagt gatacaggtg ctgcatagta ttagcgtcag
     7981 tcaactcttc tgcttgcact attaacaaac cgctggctgg cggcgagcta atccaatcct
     8041 tcattaaggg ggtagccccc gcaacagttt gcaaaaaacg gcctttacga ttactggcca
     8101 gtgccgcaga taaataacga ctcatcgcgt cacctctaca aaccagtggc aaaggtaagg
     8161 cgcattttta caatgccttt gtctacaagt cctttctgtt gctcaggtgt taacacgggt
     8221 tcctgatttt ggctcaaagc cttataaatc ggttgccaat ccgccagtgt taagcctgta
     8281 tcatttaata tttgctcacc ttgactctgc catttggcct gggtgctttc acacacctgt
     8341 tgaagttctc tgacacccaa attgctcaca tcctccagag tcaacgctgc tggttccgct
     8401 tgcttttgag aagccaatag cggttttaat aacggcagcc agctgtttac ctgtgcgatg
     8461 gagttgcgtc ttacttggtt tagacgctct tcaattttaa gtagctggcc ttgaatcggt
     8521 gtaagttgac cgcgtgccgg ttttaaaagc tcttctggta aatcccgtaa cgatgaaaac
     8581 tgcccattgc gcaaactgtt ttgcatactt tgacgcttgc tctctgcatc actggggaga
     8641 tcggcaatag catcttcaat gagctgtaat ttcattacca gttttggtag ctctgcaatg
     8701 tcatcggcga actgatactc agccgtggcg ctactaagcc attcttgcag ttgttgtgca
     8761 cgtagttttt gctctgtgag gttattaacc agcttactaa atcgttcttt gcagttgcgg
     8821 tagctggaca aaatatgccc ctgaatctcc gctatcggca actgttgatc attgtgcagg
     8881 tttaaaccca cttcttgcgc taaggctgaa agcttgtcat gcgctctacc taaatcgagt
     8941 ttacgcaggt aatgcagtaa ggtatcgcta tctgcttgct gctcttttcg gccagtggca
     9001 ctgctggtat cgtcttttaa cgctccatct aaaatatgac ctatggtgcg cagcgtgttg
     9061 gtgtacagac gtttcggaaa attcgtcagc ggggcacact ctgtttcaac atctatgatt
     9121 aaatcaacga gtcggttgtt gatacgctca gcgctgcact caacaaactc ggcaaataaa
     9181 caggcctgtt caatccgttg ataaagcgag gtcgttttgt cttctaagct gagaatatgg
     9241 gcatctttta gcataggcct ggaactatta agcggtttaa cctttgctac cagctccagc
     9301 acttcagcgc gtttgtggat aagccctgcc aatactttcg tgtcgttggc ctcgaccata
     9361 tcagccaatt gttcttcttg ctgtttgagc acgttaaata gcgattgtgc tttttccagc
     9421 tgctctttag cttccacggt gacgaaaccc agcttatttt tacccagtgg cgcgaacatt
     9481 tcattaatgc gtgcataacc atacacgcgg ctcatgactt ccacattggc tgcatagctg
     9541 cccatatcgt taccacggaa ccagttttgc gcttctctaa tggcattttc taagcgggag
     9601 cgcgggtagt gctcccaaat tgcctgtttt tctgttggat tggttaaagt gagtaagttc
     9661 agctctccac accaccacat ccagtcatta aacacgcctt tggcggtaac actcgtttca
     9721 gccacatagg cgaaataaaa ctgttgctta aagccttcga attgccactt gtgcttttta
     9781 ttagggtgag caatacgggc taaaaataat gggatctgcg cctggcttcg ctgcacatta
     9841 ttaagttcag taaatagccc ggcatgttca tcggcagtgt aacctttggc aatatagctg
     9901 cccggcacct taagtttatc caacaagctg ctcagctcat tcactggcac gccgtgttgc
     9961 tgctgtaaat cgagaatgcc atttaactca gggtgagcaa tggccagctg ataccagcct
    10021 ttaaacaaca ggtctcggtc attcggtgat aatttaccat ctacctttaa cggccaggca
    10081 attaaaccgc gctgctgcaa acgctggcgc cacttgtgaa tggcatccgt ggcaaaggtt
    10141 gtcagggcat gcagcttgtt tttgaatttc gcggtaaagc tgtcggcagt taaattcacg
    10201 ccgtgcaact gacatgctgc caaacttaag ccaatacgct ctaactggtc tgcttcgctt
    10261 gggttgacat aatgcagcag tatcgcatca cgcaactcat ttctttcatc cagtcggctg
    10321 tattgctcca tgagatgtgc agaagcagtt aacgctaata ctggcgtgcg accattttgg
    10381 tggctgtggt ttgccgcaaa ggtgtgaaga ttgttcagct caattaggtt gctgacccag
    10441 gcaaacaagg ctttaccttc tgggtgaagc ttaaatccat ccagggtaag taaaccaccc
    10501 tggcggccac ggggctcggt taaaatggcc aaaggtgcat cgtcgctgcc cgctttatta
    10561 ctcagcaagt tttcatcata ggtccagttg ttatcaagta atctcacaaa gcccgttaca
    10621 cgggctaatg ccttgatgag cggcttctgg ctactttgtt tgctaagttc actcattgcc
    10681 tgttcatgag cgtcgcccat ggcgggatct ttaaaaatca gtgagttttg ctgggcactg
    10741 cgatagcgca aatccaggcg cagtaacatc gccacactgg ggccaatatg cgttgcttcg
    10801 ctggcgtcca gttcggtagt ttcacccact agcttgtgcc ataaggcatc ggcagcgaat
    10861 tcaatcgctg ggtggtcata cagcaagctg agtaagtatt taaactcacc ttttgacaga
    10921 ggtagtaata ccgcgccttt atcgccgctt gcggcttgtt ctttaattga aaaagtacgc
    10981 aggttatcca gcaaagtatc taagttcagc gcttgatcta ctgcagggta gtaccattca
    11041 tctttatcgc ggcggaattt ggcttgctcc agggcttgtc ggcactgtag gcggtcaatg
    11101 ctcagtttgc ggtaatgagc ggctatgggt tccagatctt cgtttttcag cgccagcaat
    11161 tctgtggcat aggccatttc agctaagcgc acgggtaatt ggccgtagag caaactacga
    11221 cattcggtca atagtgcctg gtcgcgagtt tgaatgcctt ctatcgcgcc tttatccagc
    11281 acatgcctgg ctacgcggcc agagcctgcc agcacagctt caaataaatc gcccacatcc
    11341 ttgatactgc ggcctgcttg ttggtattgg ctcagcactg catccacatt agccatgacc
    11401 acgttaaacc aaccgaagtt ggcaccactc atggcgtatg cagcttccac taaaccagca
    11461 gggtaattaa aacccagctt gccgtctttt aagaggcttg ccacatagtc tgatacatcg
    11521 gcaaaggcat tatgctcaag ttccactaac tcaaagcggc gtgcgaccga ttgaatttcg
    11581 cgcaattgct ggccaagcag tggtgaacac agcgctacat agcgcaacca tggaagttta
    11641 cggcgagggt cttcctcttt aattgcttta ccaatcaggc gaatggcctg gccatcgagg
    11701 cgtttatttt gttcatcttc caggccaaag gtggcggctt ctgcaacggt ttccagctca
    11761 tccagcacca ccaacatata ctgcacgcca aagctcttta atactttata ggcggcttgc
    11821 accaaactga ctaccagcgt ttggtcagta tagaggtctt cttcgctgtg gttggcgttc
    11881 agctgtaagg catctgctaa cagttcagat ttaaagccaa atggcattaa gcgttcatag
    11941 gcttgtttgg caatttcact ttgaatcgat ccatcaaagg tttgggtggc cagtggtaat
    12001 agcgcctgat ataaaccaaa agcaaaccag ttatccgagt tttgataatc tgatgccact
    12061 tgcgagtagc gaatgtagag cgccaggtat ttgtcttgcg tgtcgcgatt aaacagctgc
    12121 ttatcgtgca gttggccttg ttcgtcgcgg acaaaccagc cccttgaaca gtcgttaatt
    12181 tgtgcgacca attcatggcc taaacgtgat ttaccccgac cccattcggc ttcgacggca
    12241 aacacatggg caaagcgatc atcgtcgtga tccaccgtat gcaaaaaggt ttgaaagcgt
    12301 ttaaaaaagc ggctctggcc cactaacggg tcgcgaggtg gataatctga tacgcccgtc
    12361 gccgcccagc gatgttgttt tgccaggcta tcaaccatct gttcgtttaa ttcaaaactc
    12421 ataacgacga tccttgaagg tttattctat tggcggcgcg ttttttattg gcatagcagt
    12481 tttgcgccag ttgcttaatg gcccgttgta acgtttggct tttgtagcca aatagtggtg
    12541 ctaacggcag gctataacag gcatcgctga agcttgtatg aattcgatat tggccggact
    12601 caccacgacc cgcctcggta aattgccaga ttttttttgc caacaattcg gcaatgaatg
    12661 agctcaggac gcggtttagg cggttgatgc tttccgtgtt cgcatcgagt gccggataga
    12721 gggggatatt gagctcaccg agcagaagca caagaaactc ggccaaacta cccagactca
    12781 cctcatcacc ttcaatgccc tgtaccgcca catttatttg accgggggca aaggcatttt
    12841 gcccggcagg taaggtgagc aacaaaccgc cacgttgctc ggcagcccag gcatcttgca
    12901 caaagataat tagactgagt aaaaataacc agggctgttg ggcgaatagc tcggtaagcg
    12961 gcacgttgtc atgacccgag cgggacacca ataaccaatc ggcattgggt ttaaggttat
    13021 gttcatcaag caaggtatta gggagtgcta acaaccgccc actacaccag ttatttgtca
    13081 gttgttttgc ggtgacatcc accgcttgta tagatgccgg tggttcattt tgttcgctca
    13141 cggtaaacag ggtatggctg gtgcgcaaaa tacggcatag agcgggaatg gcagcattgt
    13201 cgtttaggct atgaccgagt aaatcccgtt cggtttgcgc caatgggctt gcaatgattt
    13261 gtggtgagaa gtctgcgttt tccaactggg ctaagaccca ttcactggca ttgcccagtt
    13321 gctgaatgag tttaaccaat accattgggt cactcatcgc gccagcttcc tggcagcgag
    13381 cggcggctaa atttatccaa tgttggcgaa catctgggtt taagcccaac aacgctaatt
    13441 gccattcacc cgactggctt aaatcactaa aggcatcaag taatggtgca gtgaggctaa
    13501 aggcgctaat gatatccgtt tcagcgtctt tctttttcgg ctgtattatt ggttgtaaca
    13561 gtgacctgag ggcaggcaca gccgccagta acaacgctgc tttactggcg gttaaaccgc
    13621 cttgatgcca aagctgcagg cagaggttaa gtgccgcttc gctatttacg gggtcgttat
    13681 tttgcctggt tgtaatgctg tccatgcccc ttgtctccat ctttactctt cagcctccgc
    13741 ttgcgccgcc tcttccaaca aactgtataa ctcatccaca tcgctaaata gcggtgtggc
    13801 gccttctatg gcataaccct cagcgctcag tttacttaaa atcgcttgat caaagctgtt
    13861 gtcgccatct tccagggctt gttgagcctg gattaattgt tgctcggtca gttgaccttc
    13921 gatgagggct tcgacgaggg ttttggctac ttgtgttaaa aatttggcat aacgataagc
    13981 cagcctcgca ttgagcaaat aatcagcaag cttgttttct aagcttttat ccagtcgagg
    14041 aattagtacg cgaccaatat cagataggga agtatgttct acagttgttt taactgccca
    14101 ttgatttagc tgcatttgtc cttcggggcg atttagccaa tgtgttaata gttcgccacg
    14161 aatattttga ggtgagagca ttgcaacccg ttgattaacc agcataggta gctcgtcaga
    14221 tatgaaaaac tgcgagcgga aaataccgtc cataccgaca acgatcattt tgtctttagc
    14281 ttgatactta gtcgcatcct gatatacatc ttggctaacg ccagtatcta aaccaataaa
    14341 atcatcaaaa ccgatattgc ggatgcgaac taaaggtatg cctgagtttt ccaaaaagtc
    14401 gttactggat actgggcaac cagatgccaa ttcaaccaaa gtgctaatgc tgtcatgagg
    14461 tagtttttca atttcacgca ctaaacatag gtagtgagtc cgataaggac gtggatcgag
    14521 tcgatcctcc attgtttgcg cgctgactcg gtttaatagt gcggggggct cgttgatcgg
    14581 cagatttaga ctattaagat gagcgtcaac tgatgtgcga agcagcttcg cccaagcgcg
    14641 cagctgctcg gcttggcgaa ctttatcccc gatgtatttt tgaacaaact cagacacttt
    14701 cggaataggt aaacgtggaa catctaacaa gctcactcca ggaataatag tcccccggcc
    14761 tagtcgctta aaagcctttt gtccttggtc acagttcagg tagctaacga gatagtaagg
    14821 atcgctagag gccttcattt tcaggtatat gacttctgaa gaacagtttg cttcgttaac
    14881 ttcagatgga actacgcacg catttcccat atctcctttt ctaataacta aaacatctgt
    14941 tggtttgagc gctgattttg taagctcatt tttatgtgaa tcgagagata ttcgttggca
    15001 ttcatctaaa tcaatattga agcttttaat tacctttccc gaaatatatg gaatactatc
    15061 agcagcttca gagtaatacc gttcgactgc tgacgtgttt ccaataccac tcgcttcata
    15121 aagtgactgg agttccaccc agcctttgga ttttatcagt ttgtcatttt ctactaggct
    15181 cggttgataa aagttaggat caagtctttc cacaagaatt gagctgtttg ttcgaaaaaa
    15241 tttgtccatc actcaccccg cttataagcc gatacaatct tatccagatc gtttgcgaca
    15301 ccagcgttat agtcttcaat attatttttc accacgtagc ccaaggtttc agccactgcc
    15361 ataaacacgt cagtttgtgg ctcgggcaaa aattgctctg gctggttggg gttagcatgg
    15421 cgtttttgca aatacaaaat cgaggttttg gcgcccgtgc cagagagctt aaagcagtcg
    15481 ctcggcaatg aaattactgc ttttacaatg gctttaccgc caacaaactc gccggttttt
    15541 tcgtcttttt tccccatgat gtactcgcgt acataacggt cgccggagtt acataaaata
    15601 ccatcgggca gcactattaa cagacggcct ccgggtttaa gcagttgcag gcagcggtcg
    15661 ataaaaagta ctgctgggtc aatagtggca cctaccggtt gccagttgcc cttgctatcg
    15721 ggcttgctgc ccagcgcgag ccctgcggtt gtaggctgca ggctgtaaaa cggcttttta
    15781 tcagtcccgc ccgttctgcg caaatcggta cgatagcccg cccatttagg ctcgccgttt
    15841 tcatccagct ctaaatcacc gatatcctcc cacttcattt ttacgtggtc ataccaggcg
    15901 ttgtagctat caacgacttt tttggtcgga cgaaaaccac cgagcacttg ctccatattg
    15961 gcttcgtaat tggcttttga gttttgacca tttttgcctt tatcaaattt aggcgtaccg
    16021 aacggcgggt tggtgcaaat aaggtcaaag ctattcggct tcagggcgtt agtggtgagt
    16081 gagttgtcgg tatagaaaat ctgtgctttg ggggcacctt gcaatgccat attcacgcgg
    16141 gccagcatta ccatgcgtgg tgcagcatcg gcaccggtaa atgcacagtc gcgtaatttt
    16201 tgtttcaatg attctttttt atcgtcggcc agcaccagct taaaatcgat ccatttatca
    16261 atctggctta acgccaccga gccaaaacca aaggagccac aggtggggtc gcagaaacga
    16321 aaatcgccat tggctaagcg cgatcgcatt tcatcgtcat cgtcgatgtc gtgcatagca
    16381 atttctagca tggcctgttt tactgggtta ggtgttaaat aaacgccaag gccacctttg
    16441 gattcaaagt tagcgcgtag gaatacatca aacactctgc cgagtaaatc tcctgacaca
    16501 tcagccaacg tgccgtgttc tttagcgatt ttaccgtcgt tggtttttac cggtcccagg
    16561 ttttgtattg cttccagcaa atcctgatag ttttttgcaa cagacagccg caggtgtgtt
    16621 tctttactaa agatggggtt acgtgagcca tcatctgaga tcacaacgta gttttcgtgg
    16681 cttttaaatt cctcaaaggc ggctttgatt tgggcaacgg ctttatcgcc gtggctctcc
    16741 acatattgcc aattaaagac atcacgaaaa cgcagggtat ttccctcatc atctttaaag
    16801 gtaaggtctt catcgtgatg caggcggaaa gtttctaaaa acagaagctt agctacttcc
    16861 tcaataatgt cgttcttgtc gtttaccttg tctttggtaa cgcgataaac attttcatgg
    16921 aaggcatcaa atcgctgcat taagcgtgta taaacacgca tcgaccagcg aaaggttgga
    16981 tcacgctcca tgcgatcaaa gctacgtaat tcttccagac caggcagggt atctgcaccg
    17041 tgggtaccgt cagatccatc ggcaccgaac aaggccaaaa tacggtggtt caagccatcg
    17101 tccacaacgg catatactgg ccagtcacct tcgatatcac ttaacagctg ataggctaaa
    17161 tcatcaaact ttgcagtgtc agcggcgtat tcccaatggc cttctgtcag cgcaatcaac
    17221 atgactggtg gcgttaagtt gccttcaacc aggccttgaa tgagaccagc cttggcgaat
    17281 tcatgctcgg gtaattcgac caatggctga gtaaactcgg agtgctgttt gaccagttta
    17341 ttgaacagtg cttctagctg ctgttgcggg tcttgttttt ttgcttcttt tgccatgatg
    17401 ttttctaaaa tcctttaata cagccaatgg ttactacaaa tgccggctaa tttatcagta
    17461 ccagcaaggg gcctttaatc tctatctgcg cgtgcttctg tgccagtttg cgtatctgct
    17521 gttcactggt cgtaatacca gcagggagct ttttcaatac ttgtgcaagg ggcatgcgcc
    17581 caccagcttg atgcaacaat gataccaaat gtgccgcgat atcaggtata acctggtacg
    17641 ttgccgttgt gggccctttt agatgattac tttgaggcaa tgaggcaagc catagcgaaa
    17701 cttgagactg gctaaagcca agtgtttgcc agagggtttg gttctcatta aatacaacgg
    17761 cggcgcttct gggagttgct gtttgctgag ttattagttg ttccaggctt tgtcgctggt
    17821 attcatgggt catctcatgc ctccactata ggatactgaa gcagccatcc gatccagttg
    17881 ggggctcatt gtacaggaca cgtcattcgg ccacatcatc ctctcaccgc gttttgccat
    17941 tatccggaat ggcacaaccc ataattttaa agtctccgag aaagcaaaat gctgcggtat
    18001 cggttggcta aagtcatcat gcgcagggta aatcagatac atttcgccat tcccaccaag
    18061 atatttttgt ccgtaggcaa acatttgata gaagtcagct tgtgccagtc catagagtga
    18121 ttttgtctgc tggctgctat tcaccagctt ccattttgta tccatcacca tttcggtttg
    18181 aactggttta tttgattgaa tcagcaagtc ggggcgaagc ttaaagcaat ctttaactcc
    18241 atgctttacc aaagaataaa ttgctgcttg aggtaaaact ttgaggtgag gtggtaactc
    18301 gtcgggtagg gtctgtgcca caaaggattc aaataccgct tccataggga acaaaagtga
    18361 tatcgctttg gtgtttccct gcaaagcact cgggctcatt cccctcagga tcaattgcgc
    18421 ccatgccatt ggctcgttgt aatgagccat accacgctcg aggcgtaagt tgtttatatc
    18481 acgttcaata tcccggctaa gtggaatacc gtcaaacgca aaacgcagtt cgtaaagcca
    18541 gcgttgatta tctgatgaca gctgcagact aagaagctta tctagtgcgg agtgtaagag
    18601 ccgattggct gcacaatcag gcatgtagtc atcataatcg acacaaaact tatggcgatt
    18661 caccgcgtta tgtcgcagct gtgcagaaag catcagcttg cccttcataa aagccagatt
    18721 accttgcttg ctcacatagt cggagcgtaa accctgttta agcaattggc tgacgctatg
    18781 caaaaactgg ttaataaaaa tctcaagcaa aggcatacgc tgagcctgaa gagtggcttg
    18841 ctgggtttga atatgtcgga acccaggcag atggcttagc atcgtcagca atgtttgacg
    18901 tgcattagcc gctgtgaggt ttttaccaac cttgggcagt atctcaagct ggaaaccgtg
    18961 aggtgttgaa agcatccctg cgtagttttg aacctgaagc aatttgaagc ctgagcgaga
    19021 ggtgagtttg aggaactggc tcccttgatt acttgccagg ctaagcgctt ctaaataatt
    19081 aaagacctgt ggaggcaccg gttttgcgcc aactgaagcc gcttttccac tccccaagag
    19141 gtcatattca aaaacggaga taacttcacc catgatttac tgcctgctca tcaaggggag
    19201 tctgttgggg ctgataaatt gaacgataag cctgtggaat attccagatc tcttgatcga
    19261 aatctttgag ctcataagct gttgattgct gatcatggcg tcgtaaacca tggttgttac
    19321 caaaaagcgt atcgagatcg tcggttttct caatcacgaa ttgcaggttg tcgtcttgct
    19381 tttgattgtc tgccagcacc aaccggatct tgttccagtc atcgaaaaag tattcctgta
    19441 aaagcggaat gatctttttc tggaatgcga tcttcaattg tttaaacgca gcttcttcat
    19501 caccggcatc gagtgcattt tttaccggca taaagaacgc atgccccagc gtatgttcac
    19561 gatcgtaaag agcctcgatg cggctattga gtttctctaa caacgactcg agctctatgc
    19621 ctttcacctt agcttcactc agtaaagaga gatcaggcat catttcgaca aagtcaaagc
    19681 gacggcgcaa agccgtgtcc atcagagcta aagaacggtc cgctgtattc atggctccga
    19741 tgatatcgac attgccgggt acgctgaagt gatcaccgct ataagccagt tgcaggctca
    19801 tcgcattggg catgcctgca cgcttgtcta cttcaatgag tgagattagt tcaccaaaga
    19861 tcttggatat gttaccgcga ttgatctcat caatgaaaat ggcgtagcga tgtcctggat
    19921 cggcattcgc acgttggcaa aggcgcataa agatacccgg ctcaatggga taagagatat
    19981 tgccactctc gtcagagcga gcgcgtatac cttcaataaa ttcttcatag ccgtaagatt
    20041 ggtggaacgt aaccaccgca aaacgctgga tggcttcggc agatttaggg ccacgtttaa
    20101 gttcggcgta gagctctacc aaatcctcca cttgctcgag ctgtgaatca actagaaacc
    20161 attcgctgtt atctgttttg ttaaatacgg caggctcacg ccgattctta taatcaacgg
    20221 ttaacgactc gggaactgta taggattgca aagctgccca ggcagtattc gatagattgc
    20281 cattacgacc gtttaataat gccttacgtt gaaaccacat atgttcaata atttggcgaa
    20341 ctttcgctcg tttcccaaga tcgagcagca ccagcgttat aacctgcatc cagttcaacg
    20401 attcaaggcg agaatccagc taggcatcac gatcagcggg aacagcatga gaagtgtact
    20461 ctttcatttt ttgctgcaac ctgaaggtct tgccagtgcc aggggggcca aagaagatca
    20521 cgttagttgg tgaatgtagg atgatcttgt tctcgatggg ctcactgcgg gttatggatg
    20581 tagtgctagc tactttctcc tgcttttgtt cattatcgcg ttcacggagc aattcataga
    20641 gatgccagat ggccatattc acataataag gatctgtatc aggagattgg gcgtgtatgg
    20701 cttgtttcaa ttcatagttt ttctgtaacc aatttccggt cagtaccaaa ccgagatgga
    20761 agtgctgatt taagaattct gctgctttat taaaagcatt ctcatccaca gtactggtgt
    20821 agttttctgg cgagaacgcg gcaaagacgc gatttctcaa actccaatac atacgcttga
    20881 gaagtccgtt ttctttagct tgttgaagtg cattgccaac atattggtaa gtttcttcat
    20941 ccggctgttg ccgaattcgt tcagttagtt ctctaagcag tggcaggctt tgctgatatt
    21001 ctgctaagga tggaacgccc tggcgaatac ttgcaatgcc gttgtcacgc tcataccaaa
    21061 gctgtttgac cagaccatct gttgcaggct cagcaaaagt tgaggtttgc tgtagcaaag
    21121 tcgtaaatgc acggtatcgc tgataccatc tggaagctgt gtaattttta tctttcgcca
    21181 gggcatcaat caattcacta atcgttagcc tacctgcttc gacactatct acaagagtca
    21241 tcgtgacttt tcctctgttg tgacattact tattttttaa ttaatatgag gtattctaac
    21301 tatattgagt taatcgttgt gacaagtgcc tgttatatat acaactatct gttaactcag
    21361 ctctaattta aatacaatgt aaggttgtag ggtgaatgct gttatcaacc tcacccccta
    21421 aaaacctctt tccgatgcca ctctacaaac acctcataag ggtaattttt acgcacctgc
    21481 ggcaacgcaa tagttttccc gtcaaaatcc cagaataatc tctcaacgat ccctccgccg
    21541 tttaccgcat ctgataccag tacccgcata ttttcatcca ggccaatcga tcctttatca
    21601 aaggctttat ggtgaattgc gcaaagcgcc aggccattag gaatttcaca aggacctcct
    21661 tgctgtttcc attttatatg cgccgcttcc aacgctacgg tggtgtcgtc gtggcgcata
    21721 ttaaagccac atatcgcgca ctggtagtta taagcgcgta atacattttt gcgaaataaa
    21781 gggtcgcgct gtttacggat ttgctggaga tcaaaaccca gctcgtcagc aatttcttcc
    21841 tgaatactct cggtgaagtg cgcttcgagt atctgttgcg ccagagtatt gatgagtttt
    21901 ttattgccgg ttaccagggc gtagtgctgt tcatcgaaac cgcctgcaac gtggtattcg
    21961 ttcagttcct tcactggcgg ttgtcggctg ctccctgctg ttgagcaaag ctcggcattg
    22021 tgtaattgcc agaatccgtc gccttgtaat cgccagaacg gcatatcagg tcggtaatgt
    22081 gaacgctgtg gcccaaaacg ttccagtaaa ctgtgcagag gttcgtagat ttccgatcca
    22141 taatcgaaaa ggcgtggatg tccattcagg tatcccgcta atacgtatag caataacaat
    22201 ggcttatgcg gcgcgcgctg ttcacctttg tgccagattt ttatgttggc aattgcctgc
    22261 tgtagcgatt tactggaagc cattgatatc cctggcagtg aaataattgc gatcatgctc
    22321 gcaaattttc ggctgttcaa gcctgcaagt gaagatatat tactttgatc tcatttgttt
    22381 ttttgctgcc tgtgtaccct aattgccatg cgatgttgca ccgacgcaaa ccacctttta
    22441 cgccggagga gttaaaagcg ttgatggatg cgaataaatc aaattaatga acgctcactt
    22501 ttcgcgccaa aactgcaaaa ttgcttctac aaacctgtca gaaataaccc cccgacgggc
    22561 tgccgtgctg aaatgactgc ttcgcagtgc cgtcgtccag ttctccggtg accggtacgc
    22621 tgaaccgtaa tatcccgtgc ttaaatcgtc cgggtcttca tcaggcgcgt tcgtcagtcc
    22681 tctttcacct tgttgctgga agtcgacgaa aataatattg gctaaaacat tattttgata
    22741 attgccataa atagcttcat acgaatgcgg gaaattttct ttccagtacc aggtggtatc
    22801 gccgcaaaac cacggcgcgt cagtaatatt attaagctga gaatggtatt gttttagatc
    22861 cctacgaaag gcttcaacca tatgattaaa gtgttgaggg tgtgacgcgt agtcactggt
    22921 cattaagtca aattcgcctt gcatccagca tacgccgagg aatttgttct gcggattttt
    22981 tgccagtgcg gctcgcgttc tgctgactaa atcctggtat agcggagtat ccgatcccca
    23041 acgacaagca tcatggctgg ctccgtgccg ttctgaatat gtcccttcgc tgcccgcgat
    23101 aaaagccgag ccgccacgac aacatggaac gatgagaacc cctgcattat caggaataga
    23161 gggcagtaat ttccgtgcaa tatgcagtgc ctggccaacg gtgccgtact gtgtttgatg
    23221 attcgttgcc agaggatggt gataaccctg catatcctga acatcgtgtg ggcagtgagt
    23281 cagtggaata atgtcgttaa agtgacatga cgggcctccg ggatgcgtat gcgcaaatct
    23341 cgctaattgt ttaattctgg gatgaggcgc atcttccctg tccggtaatg gcagtccttc
    23401 gccatacgcc atggcattag actgaccagc aacggtaaga acatagtaat aatcgggcga
    23461 tattattgcg ttcatataaa tttcgcgtaa ttgaaatgac aaattgatag cagggcccct
    23521 ttaccggggc cctgaagcgt taattttgta ctgtgacttt attatccttc acagagatca
    23581 aaactgaatc cgtcaccgct ttgccgcctg cagtttcacc gccaataatc aatagactat
    23641 tattccaggg caatgatact ccgtaggccc gaccttgcga taattcaccc gatttatccc
    23701 atttcccgtt atgccaaaga tgaatatcag tgctatatga tttttttagg ccttcatgcg
    23761 catagttctt accgttctgg taattttctc gtgaaccttt gaatccggcc cctccggcaa
    23821 atataagaga atcattgctt atccccgcaa aaccgccagc gacgccatct ggtgatgaga
    23881 cgggagcaag cttattccat tttaaattat taccggtgaa atcaagttca aatacggcat
    23941 ccgttcgcaa tcctggtttg gcttcgccat taataagcca ggttttatca cctttattca
    24001 caagcgccgc accagccgtt ccgtaccagg gagattcgcc agcgtaactc cattgctgtg
    24061 ttgagggatc aaaagacaac agaaacttat tgaagaaata atcttctgct tttttgtcaa
    24121 aataatgggc attgatcttg tctatagttg ctgaatcttt tccagcctcg ttgagatctt
    24181 caaaatagcc attgaagata ttctggttaa caccgccagt aacataagcc ttgccattgt
    24241 gtacaaaagt cacatgaccc gccatgccca tcggtgcatg cgacatcaat ttaacccaac
    24301 tattggtttt ggggttgtat ttgtgtacgt cattaaatac ctgagtcaag cccttgctat
    24361 ttttgccaat gccgccaaac acatacagat tgccatcaat aaatgccgag gttgcttgat
    24421 ctcttggtcc accagggaat gcagctaacg ctgtccattt tttatctttg gcctgtgtat
    24481 ccagcttgta ccatgccgta cctgcgctac ctaaaccaat gtagacagtg tcgttatcaa
    24541 ttgctccggt accactttta aatggcacag gagtttcagg taatacagac gcgtttgcgg
    24601 caaatgaagc catcatgata gcaagcgccg ttattgtttt attcattgtg actgtctcct
    24661 gtctactaca gtttaaatga cacaccaatg cgataactgt tttccgataa attatctctg
    24721 ccgttgtaaa caccctgacg gtccaggtag tcatattcta tgtatggcgt aatatcgggc
    24781 gtcatatggt attgtagaac aaatgcattt tccgtcgccc atttcttatg gtttgcatag
    24841 cgataatcgt tctgtttgct gtatagcgtc gtttgccatg cgaaggtgaa atcactatta
    24901 atatggtaag tgacatatcc atcccaacga tgaacgttat cacgagacat atcaccggat
    24961 aagtcttgtt gtcggtaagc tttccagtcg taacgatagc gaatgccaaa attaagatct
    25021 tttgtcgcgt cccaggacag ttttacgtag ggtccgtagc gtgtgccgtt gctgctaaaa
    25081 tgcgttaaca ttcccgggcg caccgtccat tgatcatcaa gtttaatcgc gtaattaact
    25141 tcaacctgaa catcattgag tgcggcattt tcctttttat tatcatgaat ggtattccag
    25201 gtattacttt ccatgcttgc ccaccatcca ttttgccatc cctcactgac tttgagtcga
    25261 gtctcatagg cgtggcttcc actacgatat ccaccacgta cgtccagtgt tgcagcctga
    25321 gaaattaatg gtgacgaaaa gcacagtaat aatacgccag aaagtatttt agcctttttc
    25381 ataaatttca ctcatttgta ggatacagaa agcaatacaa agcccgcata aacaattagc
    25441 atttatgttg tgtagtattt tttgccaggc ttatagtgtc tttggcaacc gatagctgta
    25501 ttttatattt tttgtataat gtctcctgtg aaaaatctct ttccacatta tttaaataaa
    25561 ca
//
LOCUS       DAZFVO020000063        25162 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFVO020000063 DAZFVO020000000
VERSION     DAZFVO020000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 25162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.063"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKNB_RS11945"
                     /old_locus_tag="pgaptmp_002389"
     CDS             complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKNB_RS11945"
                     /old_locus_tag="pgaptmp_002389"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HGU4195886.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            747..2897
                     /gene="dinG"
                     /locus_tag="ACHKNB_RS11950"
                     /old_locus_tag="pgaptmp_002390"
     CDS             747..2897
                     /gene="dinG"
                     /locus_tag="ACHKNB_RS11950"
                     /old_locus_tag="pgaptmp_002390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HGU4195887.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKNB_RS11955"
                     /old_locus_tag="pgaptmp_002391"
     CDS             2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKNB_RS11955"
                     /old_locus_tag="pgaptmp_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HGU4195888.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKNB_RS11960"
                     /old_locus_tag="pgaptmp_002392"
     CDS             4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKNB_RS11960"
                     /old_locus_tag="pgaptmp_002392"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HGU4195889.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKNB_RS11965"
                     /old_locus_tag="pgaptmp_002393"
     CDS             5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKNB_RS11965"
                     /old_locus_tag="pgaptmp_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HGU4195890.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHKNB_RS11970"
                     /old_locus_tag="pgaptmp_002394"
     CDS             complement(5342..5602)
                     /gene="ybiJ"
                     /locus_tag="ACHKNB_RS11970"
                     /old_locus_tag="pgaptmp_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HGU4195891.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHKNB_RS11975"
                     /old_locus_tag="pgaptmp_002395"
     CDS             complement(5867..6133)
                     /gene="ybiI"
                     /locus_tag="ACHKNB_RS11975"
                     /old_locus_tag="pgaptmp_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HGU4195892.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNLRGSKDSQLR"
     gene            complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHKNB_RS11980"
                     /old_locus_tag="pgaptmp_002396"
     CDS             complement(6207..6884)
                     /gene="ybiX"
                     /locus_tag="ACHKNB_RS11980"
                     /old_locus_tag="pgaptmp_002396"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HGU4195893.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHKNB_RS11985"
                     /old_locus_tag="pgaptmp_002397"
     CDS             complement(6926..9208)
                     /gene="fiu"
                     /locus_tag="ACHKNB_RS11985"
                     /old_locus_tag="pgaptmp_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HGU4195894.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHKNB_RS11990"
                     /old_locus_tag="pgaptmp_002398"
     CDS             complement(9473..9733)
                     /gene="mcbA"
                     /locus_tag="ACHKNB_RS11990"
                     /old_locus_tag="pgaptmp_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HGU4195895.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHKNB_RS11995"
                     /old_locus_tag="pgaptmp_002399"
     CDS             10009..10935
                     /gene="rlmF"
                     /locus_tag="ACHKNB_RS11995"
                     /old_locus_tag="pgaptmp_002399"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HGU4195896.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHKNB_RS12000"
                     /old_locus_tag="pgaptmp_002400"
     CDS             complement(10932..13157)
                     /gene="ybiO"
                     /locus_tag="ACHKNB_RS12000"
                     /old_locus_tag="pgaptmp_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HGU4195897.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHKNB_RS12005"
                     /old_locus_tag="pgaptmp_002401"
     CDS             complement(13274..13996)
                     /gene="glnQ"
                     /locus_tag="ACHKNB_RS12005"
                     /old_locus_tag="pgaptmp_002401"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HGU4195898.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHKNB_RS12010"
                     /old_locus_tag="pgaptmp_002402"
     CDS             complement(13993..14652)
                     /gene="glnP"
                     /locus_tag="ACHKNB_RS12010"
                     /old_locus_tag="pgaptmp_002402"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HGU4195899.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHKNB_RS12015"
                     /old_locus_tag="pgaptmp_002403"
     CDS             complement(14791..15537)
                     /gene="glnH"
                     /locus_tag="ACHKNB_RS12015"
                     /old_locus_tag="pgaptmp_002403"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HGU4195900.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHKNB_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /gene_synonym="pexB"
     CDS             complement(15941..16444)
                     /gene="dps"
                     /locus_tag="ACHKNB_RS12020"
                     /old_locus_tag="pgaptmp_002404"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HGU4195901.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHKNB_RS12025"
                     /old_locus_tag="pgaptmp_002405"
     CDS             complement(16743..17630)
                     /gene="rhtA"
                     /locus_tag="ACHKNB_RS12025"
                     /old_locus_tag="pgaptmp_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HGU4195902.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKEIKIKELDIN"
     gene            17865..17930
                     /locus_tag="ACHKNB_RS12030"
                     /old_locus_tag="pgaptmp_002406"
     CDS             17865..17930
                     /locus_tag="ACHKNB_RS12030"
                     /old_locus_tag="pgaptmp_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195903.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            17983..18498
                     /gene="ompX"
                     /locus_tag="ACHKNB_RS12035"
                     /old_locus_tag="pgaptmp_002407"
     CDS             17983..18498
                     /gene="ompX"
                     /locus_tag="ACHKNB_RS12035"
                     /old_locus_tag="pgaptmp_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HGU4195904.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHKNB_RS12040"
                     /old_locus_tag="pgaptmp_002408"
     CDS             complement(18547..20130)
                     /gene="opgE"
                     /locus_tag="ACHKNB_RS12040"
                     /old_locus_tag="pgaptmp_002408"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HGU4195905.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHKNB_RS12045"
                     /old_locus_tag="pgaptmp_002409"
     CDS             complement(20402..20530)
                     /gene="mntS"
                     /locus_tag="ACHKNB_RS12045"
                     /old_locus_tag="pgaptmp_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HGU4195906.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20716..21183
                     /gene="mntR"
                     /locus_tag="ACHKNB_RS12050"
                     /old_locus_tag="pgaptmp_002410"
     CDS             20716..21183
                     /gene="mntR"
                     /locus_tag="ACHKNB_RS12050"
                     /old_locus_tag="pgaptmp_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HGU4195907.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHKNB_RS12055"
                     /old_locus_tag="pgaptmp_002411"
     CDS             21180..22298
                     /gene="ybiR"
                     /locus_tag="ACHKNB_RS12055"
                     /old_locus_tag="pgaptmp_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HGU4195908.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHKNB_RS12060"
                     /old_locus_tag="pgaptmp_002412"
     CDS             complement(22357..23277)
                     /gene="ldtB"
                     /locus_tag="ACHKNB_RS12060"
                     /old_locus_tag="pgaptmp_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4195909.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHKNB_RS12065"
                     /old_locus_tag="pgaptmp_002413"
     CDS             23496..25088
                     /gene="ybiT"
                     /locus_tag="ACHKNB_RS12065"
                     /old_locus_tag="pgaptmp_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HGU4195910.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLHSKGIE"
BASE COUNT         6129 a         6207 c         6696 g         6130 t
ORIGIN      
        1 tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc
       61 ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt
      121 taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat
      181 taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc
      241 gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagcg cacgcagttc
      301 tggatgctgt tcgaatttag cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc
      361 ccagttttta cgcagaggct tagaacgatc gcgccccatg cgtgcggcga ccatagggga
      421 agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata
      481 gtgttctgag gtgggccagg ttttcccgtc aaccttgatg ggccaggcgg caaagttaga
      541 aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg
      601 ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc
      661 gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct
      721 gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg
      781 ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc
      841 ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcga catctggcga
      901 ttgaagcccc caccggcgtt gggaaaacgc tctcttattt gattcccggc atcgccattg
      961 cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcattg caggatcaga
     1021 tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg
     1081 cttttgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc agtactgaac
     1141 ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccgaac aatcaggaag
     1201 agcaaaaacg ttgtgcgaag ctgaagggcg atctcgatac ttataaatgg gatggtctgc
     1261 gcgatcatac ggatatcgct ataaatgacg atctctggcg tcgtttgagt accgacaaag
     1321 ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt gttgctcgtc
     1381 gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg
     1441 aaagcgaagc ggtattgcct gacccgaaaa atttactgct ggtgctggac gaaggccatc
     1501 acctgccgga tgtggcgcgg gatgcgcttg agatgagcgc cgaaatcacc gcgccgtggt
     1561 atcggctaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc
     1621 cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt
     1681 atgagcttat cgcctcgtta aacaacattc tcaatctcta tatgcctgcc gggcaggagg
     1741 cagagcaccg ttttgcgatg ggcgaactgc cagatgaagt gctggagatc tgccagcggc
     1801 tggcaaaact caccgagatg ctgcgtggcc tggcggagtt atttcttaac gatttaagtg
     1861 agaaaaccgg cagccatgac attgtacgtc tgcatcggtt gattttgcag atgaaccgcg
     1921 cgttggggat gttcgaggcg caaagcaaac tctggcggct ggcttcgctg gcgcaatctt
     1981 ccggtgcacc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctacacctct
     2041 ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta
     2101 ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaacagtttt tcgcgtttgc
     2161 aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct
     2221 ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgcgttgag ccttccatcg
     2281 acaacgaaga gcagcatatt gccgaaatgg cggccttttt ccgtaagcag gtggagagca
     2341 aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg
     2401 actatgtgac ggatttacgt ctgatgttgc tggttcaggg cgatcagccg cgttaccgtt
     2461 tagttgaact gcaccgcaaa cgcgtcgcca acggtgagcg cagcgtgctg gtgggcttac
     2521 agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca
     2581 aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa
     2641 gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc
     2701 agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca
     2761 aacgcttgct gaccaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga
     2821 tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac
     2881 gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa
     2941 tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc
     3001 gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat
     3061 tgattgcgct gcgtatcaaa ggagaagggg aagcagagat gctcggcttt tacgaagcca
     3121 tgcaaaatca caccatcaag ctgacgccgc cagcaggcaa gccgatgccg attgtcatcc
     3181 ccagttacaa cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc
     3241 acaaactcgg ttttcctgtg gtggttcacg gggttagcga agatccaacc cgcgtgctga
     3301 ctgaaaccat ttttgaattg atgggtatta cgccaacgtt gcacggcggg caggcgcagg
     3361 cgaagctcga cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg
     3421 aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga
     3481 aactggcgac gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg
     3541 aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc
     3601 atggtactga aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg
     3661 accgtgaagg gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc gagttactgc
     3721 cacaagcaaa agatccggaa accacggcgc agtggattga gcgttgcctt gctggcagcg
     3781 aaccgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag
     3841 cggcaactat cagcgacggc ctggcgcgcg ttaatcaggc attttaattc ttttctcagc
     3901 cggatgagcc atgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat
     3961 tgaccttccc acgcccggcg tgcagcataa aaatacaaca aacacataac ataaacagga
     4021 gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc
     4081 tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat
     4141 cgcggcaaac ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg
     4201 ctcctggagt caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta aagaggcggg
     4261 ggcggcggtc acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat
     4321 ggcgctgggg attgagaaag cgcatcagca cggtattgcc gccgtggcgc tacataactc
     4381 gcatcatatc ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc
     4441 tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag
     4501 ccgctttggc accaatccgt tctgtgtggt tttccctcgt aaagataatt tcccgctgtt
     4561 gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg
     4621 cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc
     4681 ggtaatgcag gagtcgccgt tgggttcgct gttgaccttt gccgaacata aaggctacgc
     4741 ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttcc ggcggtaaaa cgacgcatca
     4801 ggaaacgtta caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa
     4861 cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt
     4921 gaaagcctcg ccgcatgatg atgataagcc gattttgcta ccgggcgagt gggaagtgaa
     4981 cacgcgtcgc gaacggcaga agcaggggat tccactggat gcgggaagct ggcaggccat
     5041 ttgtgatgca gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt tctgtcagca
     5101 gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga
     5161 tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg
     5221 acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg ataagacgcg
     5281 gtagcgtcgc atcaggcatc tgttgccgga tgcggcgtaa acgccttatc cttcctacga
     5341 attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg gaagtgatgc
     5401 tgtatccact ggcaccggct gccgcggctt tctcagccag tttcgcttcc agggcatcga
     5461 gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt gcctgggatg
     5521 ccgttaccgg ttccgcggca aacacgccaa atgacagagt tgaaagagcc atagcagcaa
     5581 caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta taagtgacgt
     5641 tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg
     5701 accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg
     5761 tacttatgga cttgttttat gcagcgtggc agtcacgaca gcgttaaagg cacggaatgc
     5821 cacgcaaaat acaaattata ctcgctatac atctcgccag ttgtagttaa cgtaactggc
     5881 tgtctttcga acctctgaga ttatatcctg tatacgctgg tttttgtaaa tctttctcct
     5941 gctgacaatg aatgcataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg
     6001 gggcaccgca ctcttcacat tcatccaggc tttcgccacg cggaatttca ccccgagcgc
     6061 gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc
     6121 aaccggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatcc
     6181 agggacgaac ttgggcagtt caagcatcag atctccgacc attcccgcag cagattatga
     6241 taaagattaa gcagcgacag gatctcttca ttttcaccat agcggctttt cagcgactga
     6301 atattgttgt ccagttcaaa cagcatggcg cgctttttat catcacggat catcgactgg
     6361 atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg
     6421 ctggagggat acaacacgag atcgcctgcc gggagtttta cccgatgttg tccgaaggtg
     6481 tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca
     6541 gaaaggtcag tacgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatgg
     6601 aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc
     6661 aaggccgcgg caaagaataa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg
     6721 gcggcgtata acgtgctgcg ggtgtcgacc tgttgattgt tcttaacttg cgcaccttgt
     6781 gcgccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaccgg
     6841 gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa
     6901 gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc aagaaggttc
     6961 ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg taatcggtat
     7021 caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga taccccagtt
     7081 tggcatcggc gacccagtaa ccttcggtaa acgctggcgt tcccaccgcg ccgtctgaac
     7141 ctttatgcat actgccgata tagcgtgcgc ccgcgccaac agagatatca tctgttgcct
     7201 gatactggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat gaggaaccat
     7261 cctgggcaac atctttgccg tttttgatgg ttgctttttg ctgggtatag ccgccaatca
     7321 cctgccacgc gggcgtgata ttcccggcca cggatatctc atagccttcg acacgtttct
     7381 taccgtattg cgagtaagtt ccgtcatcat tttgctcaac ttcattttcg atatcagtac
     7441 ggaatagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg gtgccaatct
     7501 cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta ccgctgccag
     7561 actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca tagttaatat
     7621 agacattacc gttttccgtc aggtgataca gcgccccggc tttccagttc accagattgc
     7681 ccgacttggc ggtgtcgacg gtggtgaccg gagaaccttt tgccacacca gctgggcagg
     7741 tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca gtatgataat
     7801 tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc gtatcgaagg
     7861 cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc gtcaggccgc
     7921 cgggatgaat gctgctgtca ggatgataaa tatttaccgc gggtaacgtc accggattaa
     7981 cgccgtagtt agtctgcgtt tcacgggtaa attccacgcc ggtactgaca tcatgaccga
     8041 tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt ttattactca
     8101 catctttggt attcgccgtg cgtgaccagg tccagctatt cacatcgctg gtgggctgag
     8161 taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt acgcgcgacc
     8221 aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa cgcatggtgg
     8281 cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta tgagtatcaa
     8341 cttttccgga atgattcaga gccgctgttc ccgcagatgg ggcagaataa cccggcaaac
     8401 cgatggtcgg aatgccgccg tctggcgtgt tgtgctgggt gacatgcaga taattaagat
     8461 acaaacgatt cgctgtacca aggccaaaag cgatagaagg ggcgacgccg taacgctcat
     8521 ttttgacttt gtcgcgtccg gcatcgtgcg ttttttcgcc catcacattc aggcgcaccg
     8581 cagtggtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac caggcgctgc
     8641 caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg atcatattga
     8701 tcgagcccgt cggcgcgctg cgcccgtagt cggtgccgga aggcccttta atcacttcga
     8761 cctgctcagt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaatg ccatcaatat
     8821 aaatactgtt agaggtatca gcaccgcgca tataaatggc gtcgccagtg gtggagttac
     8881 cgttctcacc cgcaaaaaac gcacccacgc cggggacgtt tttgagcgcg tcggtaaggt
     8941 tggttgcgcc ctgatcttta atcacttgtt cagaaatcac cgtcatcgtg cgggtagtat
     9001 ccgctaccgg acgcgagaat ttcggatcgg cagattgttg tggcgcataa agcgaaggcg
     9061 ttgatgcttc gacaaccagc gtgtcatccg cgttagtttg cccttcggcg gcgagtgcct
     9121 gagccacagg cgtgatgcca atacaaagac cggcaaagaa cgtgagcgaa tgaaattgtc
     9181 tggcagggaa attgcgattg ttttccattt tgcaggtgac ttttttttat atgttgaaat
     9241 tttcactgcc acgagaaagc ctctctgcgg atgcgatgcc actgttaatc tcgtgatagt
     9301 gttcgtgcaa gtgataataa ttttgataag cattatcact tggcgcgatt ttctatcacc
     9361 cattgcgcga aaaagaaata catttattta cattgcgttc atatttgatg ttttttgatt
     9421 acaaaacagg cgaaaactgc atccagaggc gaatgcagtt acgttggtgg ggttatttgt
     9481 agatggttgc agtaccgaac atctggtcat ttccgcctgc ggaattaatg acataacctt
     9541 tcgcgccttg ctcgcgcgct ttttctgcca gtttatcctc cagatcgctt aggtttgatg
     9601 cgccagttgc cgaaacggta ccggcgggcc gtagctgacc gctgtccagg ttactcatcg
     9661 gttgcgcggc ataagccgtg agagagattc cgctcaggac ggtggcaatc agtagtgtga
     9721 ggcacttttt cataatgaca tcctcataaa tacaacgggt agtgcctgat aagtgtaggt
     9781 cgcctgggcg atgaaaatga cgcaaaaaat tgacagagat gtgcttattt tttgaacgat
     9841 atatttttac aaaataagac aaatcaatga catgcataat gctgattttt atgatgaatt
     9901 gccataaacc atagccatgt taaggttttg gggaatgata aacgcagcca gatatttacc
     9961 ctgtcgcgtt atgcgggtat cttacgccgc tgcttaaagg agaatgctat gtccgcccag
    10021 aaaccggggt tgcatccgcg caaccgtcat cacagccgct acgatctcgc cacgctttgt
    10081 caggtcaatc ctgaactcag gcaattcctc acgcttacac ccgcagggga gcaaagcgta
    10141 gactttgcca atccgctggc ggtgaaggcg ctcaataagg cattgctggc ccatttttac
    10201 gccgtagcga actgggatat cccggacggt tttctctgcc cgccagtacc gggccgggcg
    10261 gattatattc atcaccttgc cgatttactg gcagaagcaa gcggaacaat tccagctaat
    10321 gccagcattc tggatatcgg cgttggtgcg aactgtattt atccgctgat tggcgtacat
    10381 gaatatggct ggcgttttac cggtagtgaa accagcagtc aggcgttaag tagtgcgcag
    10441 gcgatcatca gtgctaatcc ggggcttaac cgtgccattc gtctgcgtcg gcaaaaagag
    10501 agtggggcga tttttaacgg catcatccat aaaaacgagc aatacgacgc gaccttgtgt
    10561 aacccgccat tccacgattc cgccgctgcg gcacgggcag gtagtgagcg taaacgccgt
    10621 aacttggggc tgaacaaaga cgatgcactg aactttggtg gtcagcaaca ggagttgtgg
    10681 tgtgaaggtg gtgaagtcgc ctttatcaaa aagatgattg aagagagcaa aggcttcgcg
    10741 aagcaggtga tgtggtttac ctcgctggtt tctcgtggtg aaaacttacc gccgttgtat
    10801 cgtgctctga cggacgtggg cgcggtgaag gtggttaaaa aagagatggc ccaggggcaa
    10861 aagcagagtc gctttattgc ctggaccttt atgaacgacg agcagcgccg ccgttttgtt
    10921 aatcgccaac gttaaagcgt tggctccccc ggcggcagcg gttcagccgg ggccgcgccc
    10981 ggagcaggca gcacctgata agtctgcact ggcgcgcgaa cacctgccag gtcgaaatgc
    11041 tttttcacct ggctgtcgag ggcaaagcgg accgtccact gtttgagtgg cagcgtggtg
    11101 aacgaaacac gcagtgtaaa cgcggtattg cttaagccga caatcccggc aaagtttggt
    11161 tcaccaataa ccagcccgcg aatttcttcg ttttccatca attccgctac cgcatctttc
    11221 agtgcctgat tggctttatc agcatcttca tggcgatcaa catcgtagtt tgccaccacc
    11281 gaaccaatgc cgcggacgaa gttagcaaag gtggttatcg aagaccaagg aatgatgtga
    11341 tacgccccgg tatcctgacg cacgcccacg gagcgaatcg acatccgttc cacagtgccg
    11401 gtcaacggcc cgatagtaac caaatctcca gtgttcatgc cgttttcaaa ctgaataaat
    11461 accccggtga taatatcttt caccagcgtt tgcgaaccaa acgagatagc cagacctaat
    11521 gccccagcac ctgccagcaa tggcgcgata ttgacgccga tttccgacaa cacaatcatg
    11581 atggtgatgg tactgataat caccgccagc gcgttacgga acagcgtcag cagggtacgc
    11641 gtacgggcgc tgggtagcgg gcggccatga atatccgaag ccagccggtt ttcgatcaaa
    11701 ctggcgagca ctgtccagcc aaccgccgag aagaaaagaa tgagtgcgat gcggatcagg
    11761 atatctacgg ttttctgccc cgcgccgttt tgcagccagt tccagaaatc gaacaatccc
    11821 catgcgctca acagcaacat taccgccacg cagacggtca gaatacgcgc cgttttcagc
    11881 gccgccgaaa gccagccatt cagccgtttt tgtagctccg gatagttacg ctgagtatgt
    11941 ggcgagaggg tgatggtttt cgccagccag cgggaaaaca taccggaaac aaacgctgcg
    12001 ataccaataa tcgccaggct gcgcaccgtt gcacccatca taaatttcag gctgttgccc
    12061 ggatcgaaca acgaaaagaa aaacagcacg ataaaatagg cgcttgccag ccagtgccac
    12121 accagcgcaa aggcgcggat aaacaggctg aaaaaggcca gcgaatgctc cgcaaagttg
    12181 agcaaatgtt gggtaatctc ttttttattg cgaaagatca ggtacaacgc ccagacggtc
    12241 atgcacagca taatgatgac gttcgccagc gcacctatct gtacattcac ctgattagag
    12301 ataatcggca cggccacaat caggccataa cctatcaaac tgcttaacca gctcaggcga
    12361 cgactccagt aacgggcgct ctcgtcctga atcgtgaacg ggcgcagttc agccacgttt
    12421 gggcaaaaaa tcaggcgtag tacggctttg aaaaattcaa tcagggcaaa ggcattgaga
    12481 aacaaacttt gttggaaagc gatggtgcga ctgcctgcat tcaggttgtc gcttaatacc
    12541 tggccgacaa ataatgtcag ggccagtaac agcaggtcga taataaacgc cccgataatc
    12601 atcgccggaa gctgcaacca gttgctgcgc tcacgatttt tttgcctcgc ccactgaccc
    12661 attttgcgat acagcggcag tgcgcacagg cgaatcagcc agtaaaaacc aaacactaat
    12721 accgctaaca ttgaaaaatg ggtcagcgca ttgctgaagg tttgtggatt aaatggctta
    12781 tgcggggagc cggtgatatt gcgataaagt tgcccgaagc gggcggaaag ggcttcacca
    12841 taatggcggc tgacttcggt gaccttttgc agcacggttt gctcttcaac cagcgtcggc
    12901 ggcacgatct tcgggaccgg ttccgcaggg ggcgtcgcgg cgacagtacg caactggtcg
    12961 atcaattctt tacgcgaggt gtcgttatcc agcacatccg ccagtgcgcc ataggccgct
    13021 tttttttgtt cgatatccgg ttccggggcc ggttcagtcg ttgagtccgt tgtcgttgtg
    13081 gttgtaacgc cgggtatgga taccgcgtgg gcaggtgcgc ccagcaggca gaagaggatg
    13141 aacaggatcc accgcatgac tcctccagtg agaaaacaga gcaaaaagat aagtatagat
    13201 gctgggggag gggcgatttt aaatgagagg aatctggtgt gcctcccttt cgggtgaaag
    13261 ggaggcagag aagttaagag acgtgctgca aaaattcctg caagcgctgg ctcggcgggt
    13321 tcttgatcaa tacctgcgga tcgccatctt ccgcaatccg gcctttgtcg ataaagatca
    13381 gccgcgaagc tactttctcg gcaaaaccga tttcgtgggt cacgatcacc atcgtcatcc
    13441 cttcttcagc cagatcctgc ataaccttca gcacttcatg gcgcagttcc gggtcaagag
    13501 cggaagtcgg ttcatcaaac agcatcattt tcggcttcac cgccagcgcg cgggcaatcg
    13561 ccacacgctg ctgttgacca ccagaaagtt cggaagggta gtgatgtgca cgttctgcca
    13621 gaccgacttt cgccagcagc tcacgcgcca gtttttccgc ctcttctttg ttcgcgccac
    13681 gcacgcgtag cggaccaaac atgacgtttt ccagcgccgt cagatgcggg aagaggtaaa
    13741 actgctggaa caccatacct gcttcctggc gaatcaggcg ctcgtcaact ttcggatcgt
    13801 taaccttcag gccatcgaca atcagatcgc cggaggtgat ttcttccagt ttgttgatgc
    13861 agcgcagcag ggtcgattta ccggaaccgg acggcccgat aatcaccacg acttcgccct
    13921 gggcaatgtt caaatcgata ttgtgcagca cctgggttgg gccaaagtgc ttggagacgt
    13981 ttttaaattc aatcacagga ttttcatcct tctttccaga cgacgcagaa taaagctcag
    14041 caccagggta ataatcagat agaacaccgc cacggcgctc cagatctcaa gggcgcggaa
    14101 gttaccggca ataatttctt gcccctgacg ggtcagttcc gccacgccga tcacaataaa
    14161 cagcgaggtg tctttaatgc tgatgatcca ctggttaccc agcggcggca gcatacgacg
    14221 cagtgccagc ggtaaaatga cgtagcgaat ggtttcccaa cgtgaaagac cgagcgccag
    14281 tcctgcttca cgaaaacctt tgtggataga cagcaccgca ccacgcgtga tttccgcaat
    14341 gtacgcgccg gagttgatca tgatggtgac caccgccgca gtaaatgggt cgatgcgtaa
    14401 gtcgttaaac gccatcggca gggcgaaata gataaacatc acctggacga cgataggtgt
    14461 gccgcggatc acttcaataa agaccagcgc gacgtggttg gctatccaac caccgaaggt
    14521 gcgtgcaaaa cctgccagca atccgattac cagaccgcct gccagaccga ggaccgaaat
    14581 ccacagggtc attttggcac cttcaatcag aagcggaatg gcaggccaga tggcactcca
    14641 gtcaaactgc atatgttgtt cctgttaccg tggtgtaaat aatcaaattc agggtaatta
    14701 atggccccgg agcgggtttg cgcccctcac cctaatcctc tccccagagg ggagagggaa
    14761 ctgccagtgc gttttacagg tgtagcgtta ttatttcggt tcagtaccga accatttttt
    14821 gtagatttcg ttgtaagttc cgttctcgcg cagggttttc aacgcgccgt tgactttgtc
    14881 acgcagctcg tcgctacctt tcgggaacgc aataccgtat tgctgcgctt ccagagagtc
    14941 acctaccgct ttgaactgac cgttaccggc ggttttgatg aagtacagaa tgtttggcgt
    15001 atcgtgcaga acggcgtctg cgcggttggt gcccagttcc atataggcgt tatcgatgtt
    15061 cgggaactga cgcagatctt tagttttgat gtttgctttc gcgtaatcaa cggagccagt
    15121 accgctcttc acagcaacca ctttcccgtc gagatctttc acgcttttca catcgttatt
    15181 gttagctttc accatcacta acaggccgct tttgtagtag ccgtcagaga aatcgatcgc
    15241 ttttttacgc tcgtcggtga tggtaatgcc cgccagcgcc agatcgacgt ttttggtttg
    15301 cagtgccgga atgatcccac tgaaatccat cggcttcagt tcgtaatcca gcttcagctc
    15361 tttagcgatg gccgcccaca gatcaacgtc aaagcccaca tatttatcgc cctgtttaaa
    15421 ttcaaacgga acgaaggcgg tatccgtcgc gacaactaat tttttatccg cggcatgaga
    15481 agaaaccgca aaagccaggg tcagtgcagc cagtgaaact tttaatacag acttcatagc
    15541 atttcctttt ttctatccac gggacgatcc cctgcgtgac attcacatat atgaaaaaat
    15601 cgtgccagtt ttgcaactcc ttgatttttc gagatgcggc aaaaacgtga tgcacgattt
    15661 atagggcaat accctgaaga tgcaccattc tggggcacca atctggtgcg ctaaaattgt
    15721 gcactcaaca cagtgattat ttagcgtaaa acctgatgaa aaaacaatct ttatgtaacg
    15781 attgtgtgat gatgtggata caaaaaattt acttaatcag ctggagatag cagatggatg
    15841 cactaaataa gtgcgttgag gtgggctgca aaacaaaacg gcctcctgtc aggaagccgc
    15901 ttttatcggg tactaaagtt ctgcaccatc agcgatggat ttattcgatg ttagactcga
    15961 taaaccacag gaatttatcc aggtcgcgag acgcggcggt caggatatct gcggtgtcgt
    16021 catctttcgc ttcgccaatc gctttgcgta cgtcattagc gacgattgcg taacggtcag
    16081 ccagttcttt caggtgatcc tgaacgttgt ggatgtccag cgggtaactt ttcagcgggg
    16141 ttttgctgtt gataacttga gtggtcccca gagctacacc gcccagctgc actgcacgtt
    16201 ctgccatggt atccagatga tcgatcagtg cggtgcggaa gccatccagc atttcatgta
    16261 cggcaatgaa gttagcgccg cgcatgttcc agtgcgcttg tttggtaatc aaagaaagat
    16321 caataaactg gataacctgg cgattcagca actctactgt tgcttttttc tcgctgtcgg
    16381 agacatcgtt gcgggtataa agcagattgg tcgcttttga tttaactaat ttagcggtac
    16441 tcataatttc atatcctctt gatgttatgt cccagtaatt aacgagacta agtatagcac
    16501 cggctatgtg ttccgctatt ctggctgttc ctatcacact aatagtggta acaagcgtga
    16561 aaaacaaaac taataaagat tcaatgagtt agatatattg ataagaacaa ttcttattta
    16621 tctgctcgcc aggaattttc ggaataaata tgcttttgca taattcgata tataaagacg
    16681 gtgtagagga aaagtagcga gaaattctgc atggttatgc ataaccatgc agaaatgtaa
    16741 atttaattaa tgtctaattc ttttattttg atctctttgc gtactgtcag cgtagacccc
    16801 attgaagcgg cgatgatagc gccgagcgcc agcagctgta tgggtgtcag tgtttctccg
    16861 aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcatcag cgtaccaaat
    16921 gtccgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag agcggtcgag
    16981 agaatagcga cagccagacc caatggaata accgaccagt gccagagtgc ttcaccagcc
    17041 tgaagcgctc caattggcac gaaaattaac gctgcaatca acgaaccaat tgccaccgtc
    17101 gcagggccat gttccgctcc tgcgcgttgc ccacttaaaa tgtaaatagc ccaacaagcc
    17161 ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc ttgccccagc
    17221 ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc taccgggcga
    17281 cgagaagaga acagcgccac cgccagtggt ccggtgaact ccagcgccac cgcaataccc
    17341 agcggtactg tctgaataga aagataaaaa agataattca tcccacccag cgaaacgccg
    17401 taaaacaaca gcggtaaccg ttgctctttg gcaaagcgca gtcgccatgg cttaaagaac
    17461 gcgatgagga tcagcgttcc taatgccaga cgcagcgcag tgacacccgg tgcgcccacc
    17521 agaggaaaaa gtgacttagc taacgaggct ccaccctgaa tagacgccat ggcaacgagc
    17581 aatatgatta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat cctttctccc
    17641 acaaatatct agacttaagt aaagcgtgga gtgtactgga tatacccaat gctggttgag
    17701 catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg atggaatcat
    17761 tagtctggtg attaggaata atctggatga atgacaggga aaacatgcgt aatacttacg
    17821 cagttctctg aaaaagtgat ttaaatttag atggatagcg gtgtatggaa acgttctgtt
    17881 acatgaaatg gcccgttaga catcacaaat cgcgaagagt ttcccattaa tttttgatat
    17941 atttaaaact taggacttat ttgaatcaca tttgaggtgg ttatgaaaaa aattgcatgt
    18001 ctttcagcac tggccgcagt tctggctttc accgcaggta cttccgtagc tgcgacttct
    18061 accgtaactg gcggttacgc acagagcgac gctcagggcc aaatgaacaa aatgggcggt
    18121 ttcaacctga aataccgcta tgaagaagac aacagcccgc tgggtgtgat cggttctttc
    18181 acttacaccg agaaaagccg tactgcaagc tctggtgact acaacaaaaa ccagtactac
    18241 ggcatcactg ctggtccggc ttaccgcatt aacgactggg caagcatcta cggtgtagtg
    18301 ggtgtgggtt atggtaaatt ccagaccact gaatacccga cctacaaaca cgacaccagc
    18361 gactacggtt tctcctacgg tgctggtctg cagttcaacc cgatggaaaa cgttgctctg
    18421 gacttctctt acgagcagag ccgtattcgt agcgttgacg taggcacctg gattgccggt
    18481 gttggttacc gcttctaatc actttggtga tataaaaaat ccgcctctcg gggcggattt
    18541 ttgtttttaa ggcttcgggt cgaaaatatc ggttccgaga tggttgtaat ccaccttctg
    18601 taactggaag ttggtgatgt atatcgaccc ggctttcttc tcagatataa acgggtattt
    18661 gatgttaatt tccttcgctt taattcccgt ccactgggag aaaaaaccta agaagtcatt
    18721 ggctgagcgg cgggctttaa tcacccgatg cgctttatcg tcgctggaaa tgaccataaa
    18781 aggcacctgg aaattttgct gatacttatc atcatgggca aggtattgca cgtctttgcc
    18841 gcgctcttta aaggccagac cgtggtcaga aaagtaaacc agcgagaagc tgctgccgct
    18901 gttgcgtaac tgatcgtaca gcttgcgcag taaatcgtcc gtttgcgtca tggtatagag
    18961 atagcacgac gtttctttcg actgcacaaa ggtttcgtat ttgccttgtg tcctgtcgca
    19021 ggcctgcgga tgtgagccca tcagatgtag aacaatcagc tgcggttgcg agtgctcttg
    19081 cgccagcact tgagcggtca tatccagtaa cgcttcgtct ttcgtgtttt tatctgcttc
    19141 aaaattacct tctttcagga agtacacttc atctgcacgt ttggcgatgc tggcgatagc
    19201 agtatcgtat tcgccgattt ggccctggtt ggaaaaccac caggtctgga agcccgcgcg
    19261 atttgccagg gtgacaaagt tatcctgaaa ctgtggtttg ccatcgacta cgcgattgag
    19321 cgttaagcca agcgatttct gcgtggagcc actggcggca atgtagtcag caaatatcaa
    19381 accgttaacg ctgctggcaa acggggtatt gtcccagtga ccgccaaagg caccgagggc
    19441 atcgcgacgc gcgctttcac cgatcaccac cacataggtc tgatacttcg gcttaacggc
    19501 agtgaccgtc caggtatctt tcattccgga aagttttgcc atccgttctt gctcttcaat
    19561 cacctcgtta ttattgacga tgacatcctt agcaaaacga aataccggat agccagtatc
    19621 tttcagttta aatacgccac cccaggccag gttttgcacg ggagcaacaa aaaatgtcgc
    19681 cacactgaat accagacaca ggctgtcgaa ggtattccag cgcgcttttt cactctcttt
    19741 ttttcgcctg attgctatta cgccgagcac aaaaataaat atgccgacca tgtagctgta
    19801 ccacggaaaa atcgtcagga tttcggtcga ctcttccata ttggtggagt gcaatgccag
    19861 caatgtattg aagttaggcg cgccgtaggc ctgagcaaaa gggaaataac aagccgccac
    19921 cagcgaactg ataccgacca gaactttttg tacgcgaggc aatgttcgcc atagcaaaag
    19981 caaaatagcc gtaaacgcag cggtgtagag caaactgaag gggtaaccta agccgaggtt
    20041 aattaatagc gactgtaaaa agtagaacgc agtccacggg ctaaataccc ggctacgggt
    20101 aacaagcgat tctttgaggg ttaaattcat atgccactat cgtaacaaac gccatgtgct
    20161 taccctggcg tcaagggtca atacctgcaa gagtgcgtga agaggggaaa ggggtccgca
    20221 tccgcgagcc gcaatatacg cagggctgca agaagataga gcgagccgcc gttcaggtca
    20281 actactgacg aacaaatctt ttgcgaaggg gattgcaaat ccgacaaaaa gtggggtttt
    20341 tcagaccaaa acgcgttatg cctgcatgaa agcaggagtg aagcggcgta ccgcgacgcc
    20401 gctatttatc ggaaggttta tcttgctgcg gtttgttgtt gaccatatcg cacaacatag
    20461 agagcagcat taaccgtact ttaaagggag aatgactaaa cacgcgcata cacctcttga
    20521 actcattcat aagacctcct gacttgctaa tcccgtcgat ccttgaggga tgattgcatt
    20581 acatacagat atagcacagg ctatattata tagctattgc taaaacgtta attttttgta
    20641 ccttcgcaac tctggtttac aatgtgcgca cgaaatgaga acgctatgta tgcgtcacca
    20701 taatgaggaa gcacaatgag tcgtcgcgca ggtacgccaa cagcaaaaaa agtgacgcag
    20761 ttagtgaacg tggaagagca cgttgaaggg ttccgccagg tcagagaggc gcatcggcgc
    20821 gagcttattg atgattacgt tgagctgatt tctgacttga tcagggaagt gggggaagct
    20881 cgtcaggtgg acatggctgc tcgtctggga gtttcgcaac cgacggtggc taaaatgctt
    20941 aagcggctgg caaccatggg gctgattgaa atgatcccct ggcgtggcgt gtttttaacg
    21001 gcagaaggag agaagctggc gcaggaaagc cgcgagcgac atcagatagt cgaaaatttc
    21061 ttgttggtgt tgggcgtcag tccggaaatc gcccgtcgcg acgcggaagg catggagcac
    21121 catgttagtg aagagacgct cgacgctttt cgtttgttta cccagaaaca cggtgccaaa
    21181 tgagcctgcc ttttttacgc acgctgcaag gcgatcgttt ttttcagtta ttaattcttg
    21241 ttggtatcgg attaagcttt ttcgtgccct ttgcaccgaa atcctggcct gctgctatcg
    21301 actggcacac catcatcacc ttaagcggcc tgatgctgct gaccaaaggt gtggagttaa
    21361 gcggttattt tgatgtgctg gggcgcaaaa tggtgcgccg ctttgctacg gagcgtcggc
    21421 tggcgatgtt tatggtgctg gcggcggcgc tgctttctac ctttctgacc aacgatgtcg
    21481 cgctgtttat tgttgttccg ctgactatca cgctaaaaag actgtgtgag atcccggtta
    21541 atcggctgat tatttttgag gcgctggcag tcaacgctgg ttcgctactg acgccaattg
    21601 gcaacccgca aaatattctt atctggggac gttctggtct ttcgtttgcc ggatttattg
    21661 cccaaatggc accgctggct ggcgcaatga tgctgacgct cctgctcctg tgctggtgtt
    21721 gtttccctgg aaaggcgctg caataccata cgggggtgca aacaccggag tggaaaccgc
    21781 ggctggtgtg gagttgtctg gggctgtata tcgtctttct gacggcgctg gagttcaaac
    21841 aagagctgtg gggactggtg attgtggcgg caggctttgc gctgctggca cgtcgcgtgg
    21901 tgctcagtgt ggactggacg ctgctgctgg tgtttatggc gatgtttatc gacgtccatt
    21961 tactgaccca gcttccagcg ttgcaaggcg tgttgggtaa cgtgagtcat ctatctgaac
    22021 ccgggttatg gttaacggca atcggtttat cgcaggtgat cagtaacgtg ccgagtacca
    22081 tattgttgct gaactatgtg ccgccgtctt tattactggt atgggcggta aacgtaggtg
    22141 gctttgggtt attacccgga tcgctggcaa atttgattgc gctacgtatg gcgaacgatc
    22201 gccgcatctg gtggcgtttc catctctatt caataccgat gctgttgtgg gcggcgttgg
    22261 tgggatatgt tttgttagtt atactcccgg ccaactaggg tctggcaaat aaaaaaggcg
    22321 gattatgagt ccgccttttg ctttaagttt tgaagattaa ttcagacgaa ccggcatccc
    22381 ggagcggttt ttaatcgctt catcaagaac aacctggtca acatctggct gaccggtcac
    22441 tgtttgcacg ctcttcgtca gggtaattgg cacaatttcc tgaccttcaa actgggcttc
    22501 ggtggtagac agcgggttat ggacttcaat ataacggctg ccgtctggct cggtggtcgc
    22561 ttttaccggc tcatcaataa actgtacgcg ggtaccgacc ggtactttct cgaacaggaa
    22621 tttgatgtct tcgttacgca gacgcacaca accatgactt acacgcaggc cgataccgaa
    22681 gttggcgttg gtgccatgga tagcatacag gcgaccgatg tagagtgcat acagacccat
    22741 cgggttatcc ggacctgccg gaacgacagc cggaagcggt tcgcccgcag cgcggtactc
    22801 tgcgtgcatt ttggcggtcg gcgtccaggt cgggcctgct ttcttacgct caactttggt
    22861 ggtccagttg ataggcgtat ctttgcctaa ctgaccaatg ccgatcggca gcacgataac
    22921 ggtgttggtc cctttcggat agtaatacag acgcatctct gcactgttaa tgacgatgcc
    22981 ttcatgaacg gtatccggca ggatcagctg ctgcggaatg ttcagtacag taccgccttt
    23041 cggcaggaag gtatccacac ccgggttcgc ttccatcata ttggaaagcc ccatctggta
    23101 ctccgcggca aaatactcca gcggctgagt gttaccttca ggaatggtga tcacctgatt
    23161 ctgaccaacc aggcgactcc cgtcggttgg cagaggataa gttaccgcag aggcggtact
    23221 gcaaaagccg acaacagcga aggccgctgc gaataatgtt ttcaatttca tattcatgtt
    23281 aagcgagatt ttgtgcctgg caggccattg ggttgagaat attagagtat tggaagcgca
    23341 ttataagttc attccagctc acagtgaaat cagatgtgta cgaaatcaca ttttttgcct
    23401 ttggcttgag tgtagacctt aagcgaggag caggatcttc tttcagactt atggcataat
    23461 gcgcggtttg tcatatctct tttcaggata cgcctgtgtt agtttccagt aacgtcacca
    23521 tgcagttcgg cagtaagccg ttgtttgaaa acatttccgt caaatttggc ggcggcaacc
    23581 gttacggcct gattggcgcg aacggtagtg gtaaatccac ctttatgaag atcctcggcg
    23641 gcgacttaga gccgactttg ggtaacgttt ccctcgatcc caacgagcgc attggtaagc
    23701 tgcgtcagga tcagtttgcc tttgaagagt tcactgtgct ggatacggtg atcatggggc
    23761 ataaagagtt gtgggaagtg aagcaggagc gtgaccgcat ctatgctttg ccggaaatga
    23821 gtgaggaaga cggctataaa gtggccgatc tggaagttaa atacggcgaa atggacggtt
    23881 actctgcgga agctcgcgcc ggtgaattgt tgcttggcgt gggaattcca gtggaacagc
    23941 actacggccc gatgagtgaa gttgctcctg gctggaagct gcgtgtgctt ctggcgcagg
    24001 cgctgtttgc tgatccggat attctcctgc tcgacgaacc gaccaacaac ctcgacatcg
    24061 acaccattcg ctggctggaa caggtgctga acgagcgtga cagcaccatg atcatcatct
    24121 cgcacgaccg tcacttcctt aacatggtct gtacccacat ggcggatctg gattacggcg
    24181 agctgcgcgt ttatccgggt aactacgatg agtacatgac ggcggcgacc caggcgcgtg
    24241 aacgtctgct ggccgataac gccaagaaga aagcgcagat tgctgagttg caatctttcg
    24301 ttagccgctt tagcgccaac gcctcgaaat ctcgccaggc aacttcgcgc gcgcgccaga
    24361 ttgataaaat caaactggaa gaggtgaaag cctccagccg tcagaacccg ttcatccgtt
    24421 ttgaacagga taagaaactg ttccgtaacg cgctggaagt ggaaggtctg accaaagggt
    24481 ttgataacgg tccactgttt aaaaatctca acctgctgct ggaggtcggt gaaaaacttg
    24541 cggtactggg taccaacggc gtcggtaaat caacgctgct gaaaacgctg gtgggcgatc
    24601 tgcaaccgga cagcggcacc gtaaaatggt ctgaaaacgc gcgcattggt tactacgccc
    24661 aggaccacga atatgagttt gaaaatgatc tgaccgtgtt cgaatggatg agtcagtgga
    24721 agcaggaagg cgatgacgag caggcggtac gcagcattct cggtcgtttg ctgttcagcc
    24781 aggacgacat caaaaagcca gctaaagtgc tttccggtgg ggaaaaaggg cggatgctgt
    24841 ttggtaagtt aatgatgcag aagccgaaca ttctgatcat ggacgaaccg accaaccacc
    24901 tggatatgga atccatcgag tcactgaaca tggcgctgga actgtatcag ggcacgctga
    24961 tctttgtttc acacgaccgt gagttcgtaa gctccctggc gacccgcatt ctggaaatca
    25021 ccccggaacg cgtgatcgac tttagcggta attacgaaga ttacctgcat agtaaaggga
    25081 tcgagtaagt gattatccgt caccccgata ctgtcggggt gacggcggct gatgacaaat
    25141 gcaaaatagc ctgatgcgct ac
//
LOCUS       DAZFVO020000064        24669 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFVO020000064 DAZFVO020000000
VERSION     DAZFVO020000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.064"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..592
                     /locus_tag="ACHKNB_RS12070"
                     /old_locus_tag="pgaptmp_002414"
     CDS             <1..592
                     /locus_tag="ACHKNB_RS12070"
                     /old_locus_tag="pgaptmp_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGU4195911.1"
                     /translation="SAYLVGDPLEFGKKLGNAAADYFIANKIDQPKIAVINCEAFEVC
                     VQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLNAIMGESGGATL
                     DAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKMGNAVFAQTLKV
                     INKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            615..2126
                     /locus_tag="ACHKNB_RS12075"
                     /old_locus_tag="pgaptmp_002415"
     CDS             615..2126
                     /locus_tag="ACHKNB_RS12075"
                     /old_locus_tag="pgaptmp_002415"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HGU4195912.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQTLGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2151..3149
                     /locus_tag="ACHKNB_RS12080"
                     /old_locus_tag="pgaptmp_002416"
     CDS             2151..3149
                     /locus_tag="ACHKNB_RS12080"
                     /old_locus_tag="pgaptmp_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4195913.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3182..4218
                     /locus_tag="ACHKNB_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /pseudo
     CDS             3182..4218
                     /locus_tag="ACHKNB_RS12085"
                     /old_locus_tag="pgaptmp_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            4230..5102
                     /locus_tag="ACHKNB_RS12090"
                     /old_locus_tag="pgaptmp_002418"
     CDS             4230..5102
                     /locus_tag="ACHKNB_RS12090"
                     /old_locus_tag="pgaptmp_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HGU4195914.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5150..5572)
                     /locus_tag="ACHKNB_RS12095"
                     /old_locus_tag="pgaptmp_002419"
     CDS             complement(5150..5572)
                     /locus_tag="ACHKNB_RS12095"
                     /old_locus_tag="pgaptmp_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4195915.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(5669..6871)
                     /gene="hcaD"
                     /locus_tag="ACHKNB_RS12100"
                     /old_locus_tag="pgaptmp_002420"
     CDS             complement(5669..6871)
                     /gene="hcaD"
                     /locus_tag="ACHKNB_RS12100"
                     /old_locus_tag="pgaptmp_002420"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HGU4195916.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(6881..7693)
                     /gene="hcaB"
                     /locus_tag="ACHKNB_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(6881..7693)
                     /gene="hcaB"
                     /locus_tag="ACHKNB_RS12105"
                     /old_locus_tag="pgaptmp_002421"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HGU4195917.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(7690..8010)
                     /locus_tag="ACHKNB_RS12110"
                     /old_locus_tag="pgaptmp_002422"
     CDS             complement(7690..8010)
                     /locus_tag="ACHKNB_RS12110"
                     /old_locus_tag="pgaptmp_002422"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HGU4195918.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8010..8528)
                     /gene="hcaF"
                     /locus_tag="ACHKNB_RS12115"
                     /old_locus_tag="pgaptmp_002423"
     CDS             complement(8010..8528)
                     /gene="hcaF"
                     /locus_tag="ACHKNB_RS12115"
                     /old_locus_tag="pgaptmp_002423"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HGU4195919.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(8525..9886)
                     /gene="hcaE"
                     /locus_tag="ACHKNB_RS12120"
                     /old_locus_tag="pgaptmp_002424"
     CDS             complement(8525..9886)
                     /gene="hcaE"
                     /locus_tag="ACHKNB_RS12120"
                     /old_locus_tag="pgaptmp_002424"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HGU4195920.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10022..10912
                     /gene="hcaR"
                     /locus_tag="ACHKNB_RS12125"
                     /old_locus_tag="pgaptmp_002425"
     CDS             10022..10912
                     /gene="hcaR"
                     /locus_tag="ACHKNB_RS12125"
                     /old_locus_tag="pgaptmp_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HGU4195921.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11072..12211
                     /locus_tag="ACHKNB_RS12130"
                     /old_locus_tag="pgaptmp_002426"
     CDS             11072..12211
                     /locus_tag="ACHKNB_RS12130"
                     /old_locus_tag="pgaptmp_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HGU4195922.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12203..13483)
                     /gene="csiE"
                     /locus_tag="ACHKNB_RS12135"
                     /old_locus_tag="pgaptmp_002427"
     CDS             complement(12203..13483)
                     /gene="csiE"
                     /locus_tag="ACHKNB_RS12135"
                     /old_locus_tag="pgaptmp_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HGU4195923.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13674..14528)
                     /locus_tag="ACHKNB_RS12140"
                     /old_locus_tag="pgaptmp_002428"
     CDS             complement(13674..14528)
                     /locus_tag="ACHKNB_RS12140"
                     /old_locus_tag="pgaptmp_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4195924.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(14673..15476)
                     /gene="suhB"
                     /locus_tag="ACHKNB_RS12145"
                     /old_locus_tag="pgaptmp_002429"
     CDS             complement(14673..15476)
                     /gene="suhB"
                     /locus_tag="ACHKNB_RS12145"
                     /old_locus_tag="pgaptmp_002429"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HGU4195925.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            15595..16332
                     /gene="trmJ"
                     /locus_tag="ACHKNB_RS12150"
                     /old_locus_tag="pgaptmp_002430"
     CDS             15595..16332
                     /gene="trmJ"
                     /locus_tag="ACHKNB_RS12150"
                     /old_locus_tag="pgaptmp_002430"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HGU4195926.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            16633..17121
                     /gene="iscR"
                     /locus_tag="ACHKNB_RS12155"
                     /old_locus_tag="pgaptmp_002431"
     CDS             16633..17121
                     /gene="iscR"
                     /locus_tag="ACHKNB_RS12155"
                     /old_locus_tag="pgaptmp_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HGU4195927.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            17233..18447
                     /gene="iscS"
                     /locus_tag="ACHKNB_RS12160"
                     /old_locus_tag="pgaptmp_002432"
     CDS             17233..18447
                     /gene="iscS"
                     /locus_tag="ACHKNB_RS12160"
                     /old_locus_tag="pgaptmp_002432"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGU4195928.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            18475..18861
                     /gene="iscU"
                     /locus_tag="ACHKNB_RS12165"
                     /old_locus_tag="pgaptmp_002433"
     CDS             18475..18861
                     /gene="iscU"
                     /locus_tag="ACHKNB_RS12165"
                     /old_locus_tag="pgaptmp_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HGU4195929.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            18878..19201
                     /gene="iscA"
                     /locus_tag="ACHKNB_RS12170"
                     /old_locus_tag="pgaptmp_002434"
     CDS             18878..19201
                     /gene="iscA"
                     /locus_tag="ACHKNB_RS12170"
                     /old_locus_tag="pgaptmp_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HGU4195930.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19297..19812
                     /gene="hscB"
                     /locus_tag="ACHKNB_RS12175"
                     /old_locus_tag="pgaptmp_002435"
     CDS             19297..19812
                     /gene="hscB"
                     /locus_tag="ACHKNB_RS12175"
                     /old_locus_tag="pgaptmp_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HGU4195931.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            19829..21679
                     /gene="hscA"
                     /locus_tag="ACHKNB_RS12180"
                     /old_locus_tag="pgaptmp_002436"
     CDS             19829..21679
                     /gene="hscA"
                     /locus_tag="ACHKNB_RS12180"
                     /old_locus_tag="pgaptmp_002436"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HGU4195932.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            21681..22016
                     /gene="fdx"
                     /locus_tag="ACHKNB_RS12185"
                     /old_locus_tag="pgaptmp_002437"
     CDS             21681..22016
                     /gene="fdx"
                     /locus_tag="ACHKNB_RS12185"
                     /old_locus_tag="pgaptmp_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HGU4195933.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22028..22228
                     /gene="iscX"
                     /locus_tag="ACHKNB_RS12190"
                     /old_locus_tag="pgaptmp_002438"
     CDS             22028..22228
                     /gene="iscX"
                     /locus_tag="ACHKNB_RS12190"
                     /old_locus_tag="pgaptmp_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HGU4195934.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22406..23689
                     /gene="pepB"
                     /locus_tag="ACHKNB_RS12195"
                     /old_locus_tag="pgaptmp_002439"
     CDS             22406..23689
                     /gene="pepB"
                     /locus_tag="ACHKNB_RS12195"
                     /old_locus_tag="pgaptmp_002439"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HGU4195935.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            23831..24607
                     /gene="sseB"
                     /locus_tag="ACHKNB_RS12200"
                     /old_locus_tag="pgaptmp_002440"
     CDS             23831..24607
                     /gene="sseB"
                     /locus_tag="ACHKNB_RS12200"
                     /old_locus_tag="pgaptmp_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HGU4195936.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
BASE COUNT         5749 a         6188 c         6943 g         5789 t
ORIGIN      
        1 ctcggcgtat ctggtcggcg atccactgga atttggtaaa aaactgggta acgctgccgc
       61 cgattatttt attgccaata aaattgacca gccgaaaatt gccgtcatca attgcgaagc
      121 ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa gtattaaaat cccgcgttcc
      181 cggcgcgcaa attgtcgcta atcaggaagg gactgtttta gataaagcga tttccgttgg
      241 tgaaaaactg attatctcca cgccggatct caacgccatt atgggggagt cgggcggtgc
      301 gacactcgac gcggtaaaag cggtacgtaa tcaaaatcag gccggaaaaa ttgctgtttt
      361 cggttcggat atgacaaccg aaattgctca ggagctggaa aacaatcagg tgctgaaagc
      421 ggtagtggat atttccggta agaaaatggg caatgctgtt ttcgcgcaaa cattgaaggt
      481 tatcaataaa caagccgacg gtgaaaaagt gattcaggtg ccaatcgatc tctataccaa
      541 aacggaagat ggtaaacagt ggctggcaac gcacgttgat ggtctgccct aatcgctgat
      601 tcgcagaggt ccctatgttc acggcaacag aggcagtccc ggtagcaaaa gtggtggcag
      661 gaaataagcg ttaccccggc gtcgttgcgc tggataacgt taacttcacg ctcaataaag
      721 gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa atcgactctc attcgaatgc
      781 ttaccggcag cgaacgcccg gatagcggtg atatctggat tggcgagacg cgactggaag
      841 gtgacgaagc tacgttgact cgccgtgccg ctgaactggg ggttcgtgcg gtttatcagg
      901 aattaagtct ggtggaaggg ctgacggtgg cagaaaacct ctgccttggt caatggcctc
      961 gccgcaacgg catgattgat tacctgcaaa tggcgcagga tgcccaacgt tgcttacaga
     1021 cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac gctaagcccg gcgcaaaagc
     1081 aactggtgga aattgcacgg gtgatgaagg gcgagccgcg cgtggtcatt cttgatgaac
     1141 ccaccagctc gcttgccagt gcagaagttg aactggtgat cagtgcggtg aaaaagatgt
     1201 cggcactggg cgtggcggtg atttatgtca gccaccggat ggaagaaatc cgccgcattg
     1261 cctcctgtgc caccgttatg cgcgatggtc aggtggcggg cgatgtgatg ctcgaaaaca
     1321 cctccacgca tcatattgtg tcgctaatgc tagggcgtga tcacgttgat attgcgccgg
     1381 tagcccctca ggaaattgtg gatcaggccg tgctggaagt ccgtgcatta cgccataagc
     1441 ccaagctgga ggatatcagc tttacgttac gtcgcggcga agtgcttggc attgctggcc
     1501 tgctgggggc agggcgcagt gaattgctga aggcgattgt tgggctggag gagtatgaac
     1561 agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc cgattacggc gacatgctga
     1621 aacgcggcat tggctatacg ccagaaaacc gcaaagaagc ggggatcatt ccctggttgg
     1681 gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat cagcgccaac ggcgtactgc
     1741 aatggtccac catccgccgc ctgaccgaag aggtgatgca gcggatgacg gtcaaggccg
     1801 ccagtagcga aacgcccatc ggcacgcttt ctggcggcaa tcagcaaaaa gtggtgatcg
     1861 gtcgttgggt ctatgccgcc agccagattt tgttgctcga cgagccaacg cgcggcgtcg
     1921 atatcgaagc caaacagcag atttaccgta ttgtccgtga gctggctgcc gaaggaaaaa
     1981 gcgtggtgtt tatctccagt gaagtggagg agttgccgct ggtgtgcgac cgcatcctgt
     2041 tgttacagca cggtacgttc tcgcaggagt ttcactcgcc ggtcaatgtg gatgagctga
     2101 tgtccgccat tctgtctgtg cactgatgaa ttttaccgag gaacctgaag atgtctgctt
     2161 cgtcattatc attgccgcag ggcaagagcg tctcgctcaa acaatttgtc agtcgccata
     2221 ttaatgagat cggtttgctg gtggtaatcg ccattcttta tctggtcttc tccctgaacg
     2281 cgcctggctt tatctcattg aataaccaga tgaacgtgct gcgcgatgcc gccaccattg
     2341 ggattgccgc ctgggcgatg acgctgatta ttatctccgg tgaaattgat gtcagcgttg
     2401 ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt gctgcaattt gaagttccgc
     2461 tggcggtggc gtgtctgctg gtgttgctgt taggcgcgct gatgggaacg ctggccgggg
     2521 tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac actggggctg tggagcgccc
     2581 tgcgcggaat ggggctgttt atgacgaacg cactgccagt gccgattgac gaaaacgagg
     2641 tgctggactg gctgggcgga caatttctcg gtgtgccggt atccgcgctg atcatgattg
     2701 tgttgtttgc gctgtttgtg ttcatcagcc gcaaaaccgc cttcgggcgc tcggtttttg
     2761 ccgttggcgg taatgctacg gcggcgcagt tgtgcggcat caacgttcgt cgggtgcgca
     2821 ttcttatctt taccctttcg ggattattag cggcggtgac cggcattttg ttggcggcgc
     2881 gcctcggttc tggtaacgca ggtgccgcaa acggtctgga gtttgacgtc atcgccgcgg
     2941 tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc cctgttcggt acattgcttg
     3001 gtgtgctggt gattacgcta atcggtaacg gtctggtgct gctcggtatt aactcctttt
     3061 tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt gctggcgaat atcttgctga
     3121 cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc cctggcgcgg gtgcggcagg
     3181 gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa acgatgctgg cagcttattt
     3241 accaggaaat tcaaccgtcg atctgcggga agttgcggaa gcgatgtcca ctatatctat
     3301 catcaacacc gtgccacggc ggcggcaccc gataaaccgt tataccaggg atttatcaac
     3361 ggtcatgaac cgtgcgggca gattgtggcg atggggcaag gctgtcgcca ttttaaagag
     3421 ggtgatcgcg tgctggtgta tcacatttct ggctgtggtt tttgcccgaa ctgccgccgc
     3481 ggctttccta tctcttgtac tggcaaagga aaagcggctt acggctggca gcgtgacggc
     3541 ggtcatgccg aatatctgct ggcggaagaa aaagatctga tcctcctgcc ggatgcgctg
     3601 agctacgaag atggtgcgtt tatcagttgc ggcgttggca cggcctatga agggattttg
     3661 cgcggcgaag tttccggcag tgataacgtg ctggtggtcg gtctggggcc agtcggcatg
     3721 atggcgatga tgctggcgaa aggtcgcggt gcaaaacgga tcatcggcgt tgatatgctg
     3781 ccggaacgtc tggcgatggc aaaacagtta ggggtgatgg atcacggcta tttagcaacc
     3841 accgaaggtc tgccgcagat tatcgccgaa ctcacccacg gtggcgcgga tgttgcgctc
     3901 gattgttctg gtaatgccgc aggtcgcttg ctggcactgc gatccaccgc tgactgggga
     3961 cgggtggttt acatcggaga aaccggaaaa gtggagttcg aggtcagcgc cgatctgatg
     4021 caccatcaac ggcggattat cggctcctgg gtgaccagtc tgttccatat ggaaaaatgc
     4081 gcccatgatc tgacggactg gaaactgtgg ccgcgtaacg ccattaccca tcgcttctcg
     4141 ctggaacagg caggtgatgc ctatgcgctg atggcgagcg gcaaatgcgg gaaagttgtg
     4201 attaacttcc cggattaagg agttatcgga tgaccatcta taccttatcc cacgggtcgc
     4261 taaagctgga cgtttctgac cagggcggcg taatcgaagg cttctggcgc gatacgacgc
     4321 cgctcttacg tcctggtaaa aaaagcggtg tggcaaccga tgcctcatgt tttccgctgg
     4381 tgccatttgc aaaccgggtg agcggcaatc gttttgtctg gcaggggcgt gagtatcaac
     4441 tgcaaccgaa tgttgagtgg gatgcgcact atctacacgg cgatggctgg ctgggcgagt
     4501 ggcaatgtgt ctcgcacagc gatgatagtt tgtgtctggt gtatgaacat cgcagcggtg
     4561 tctatcacta tcgggtaagt caggcgtttc atttaacggc ggatacgctg acggtgacgc
     4621 tctctgtcac caatcaaggg gcagagacgc tgccatttgg taccggctgg catccttatt
     4681 ttccgttgtc gccgcaaacg cggattcagg cacaggcgag cggttactgg ctggagcggg
     4741 agcagtggct ggcgggtgag ttttgcgagc agctaccgca ggaactggat tttaaccagc
     4801 ccgcgccgtt gccgcgccag tgggtgaaca atggttttgc cggatggaat ggtcaggccc
     4861 gcatcgagca gccgcaggaa ggttatgcca tcatcatgga aacgacgcca cctgcaccgt
     4921 gttatttcat ctttgtttcc gaccctgcgt ttgataaagg atatgcgttt gatttcttct
     4981 gtctggaacc gatgagccat gcgccggatg atcatcatcg cccggaaggg ggtgatctca
     5041 ttgcgctagc accaggggaa tcaacaactt cagagatgtc gttgcgggta gagtggctgt
     5101 aatttattaa atgccggata aggcgttcac gccgcatccg gcatctttcc taacgccgat
     5161 cgagagaaat tgcccccggc ccggtaattg ccagcagcag gaatgcgcca gcgatactga
     5221 cattcttcca gaaattaatc atatttggcc caaccgcatc gccggtcata tcccagtaat
     5281 ggtgaccaat caccgccgta cccagcgtgt agaaaataaa cagcaccgcc agcggacggg
     5341 tgaaaaagcc aagcacgatt aatatcgcgg cgggcacttc cataactacc gcaataatcg
     5401 ctgccagcat cggcattggc gcgcccaacg aggccatata ttggaccgta ccgtcaaagc
     5461 ccatcatttt gggaaaacca aaaataatga aaattaagac cacggcgata cgggcaatta
     5521 ataacaaaac ggggcgggca gctccaaaat caaaataacg tagtgtgttc ataaccaatc
     5581 cctttgcaag ataatgtgat ttaaacgtaa tacacactct tgcacctcgc cacgccccag
     5641 gcattgagat attcgctaat tatcctggtt acagtgattt aagcgcgatg ttctcatcta
     5701 tcagcagttt cgcatcaaac gttttgccgc tctggatcca tttgcgaatt gggcgaatct
     5761 cacgcccctg attcagcgtg accgcaccga taagcacgcc gttttgcaga ttaaaccaaa
     5821 tcgccttctg agtttccggg ttgccacgac aaagccagtc atcgccacgc atatcgccaa
     5881 taaactgtaa gttatcactg tactgatcgc tccagaacca cggcggcggc agtagcggta
     5941 gcggtagccc taacattgcg gcagcggcaa tttgcgcctg gttattggcg ttttcccagc
     6001 tttcgcagcg gtgtagtgca ccattatcaa gacgagtgat tgccacatcg ccaccggcaa
     6061 agatcgcggg atcgcaggtg cggcaagcct catcaatgac aatgccattg gcagtatcaa
     6121 ggttggcctc gcgagccagt tgctcgttgg cgctgatacc aataccgtaa atcaccacat
     6181 cagcctgaag cgtttcccca ctttgcagcg tcagttctac tttttcacca tcgaccacat
     6241 gttcaatggc attattgagc agaatgcgca caccagcctg ctggtggcgc tgtaaaagat
     6301 agcgttgcac gggcggtggt gcattacggc ccatgacggt tgccgccagt tcaatcactg
     6361 tcaccttaca tctgcgctgc gtggcgctgg cagccagttc cagaccaata gttccggcac
     6421 cgacaatcac gactgaccgt tcgggctgca gaacttctcg cagtctggcg gcatcaccgg
     6481 catggcgtag ggtaaagcag cgttctccca gtgcatcaag caacggcagc ggtcgagctg
     6541 ccgcgccggt tgctataaaa agctgatccc agtgccagct ttcgccgttg gttaacacta
     6601 actctcgtgt gtcgcgaccc agcgttttga tggttacacc ggaatgcaga tgaacattgt
     6661 tttcctgcca ccagttagcg ggtaacacct gctgtaactg cggggaatct tccagcaaca
     6721 tggatttcga gagtggaggt cgttcataag gaagatgtcg ctcatcggaa aacagatgca
     6781 gctcaccggt gaacccttgc tggcgtagcg aggccgcagc cattgccgcc gcttgcccgc
     6841 caccgacaat aatgatcgtt ttttctttca tcgtgcttcc ttaaagatcc agcccagccg
     6901 ctacgtggcg aatgccgcga atcgctaaac ccgcatcagc gttgatcatc acaccgctta
     6961 atgcgcgatt attgcgccgc gatgccaaca tcacatacgg ccccgtaaaa tccgccggtt
     7021 gcgggaaaaa ttgcagcggt aaaatggcgg caattttctc cggcgtcaga gactgcatta
     7081 tcgaggtttc actttgcccg agcgcctgtg ggccgcgcag gtcgctggcc ataccacacg
     7141 ggccgacgcc attcacccgc actttcggtg ccagttcata agccagttgg cgaataagtc
     7201 cggttgcggc atgtttactg gcggtgtaca gcgggccacc gccgccagga taccaggcgg
     7261 catttgacag tgtgaaaatc atgctgcctt cactggcgat taacgccgga gcgcaggctt
     7321 ttgcgcccag caggtaaccg agaacattga cgttaaatag ctcgtggaag ccggtttcga
     7381 gcgtctctgc gggagtatta accagtgagg cattgtgatc ccagatgccc gcattgccga
     7441 taaaacaatc cagcttgccg gaacgagtca ggatctgatc gaccgcgcgt tgataatcgg
     7501 cataacaggt tacgttacct tccaccgcca gaatatgttc gccaaatcgc tgacgcagac
     7561 tggcgacttt tgccgccgac agttccagcg tggcaacctg cgcgccttct tcgataaatc
     7621 gctcgaccag cgccagccct aatcccgatc cgccgccggt aataaaaatg gactcgttat
     7681 gcagatcgct catggctgcg cctccggtaa gtcgatgaaa atgtcaccac cttcaacgtg
     7741 tactggataa gtggtgagcg gatcggtggc gggcaggcat aacgctttcc ccgttttcag
     7801 gcaaaaactg gcggcgtgta gcgggcactc caccgtggcg tcatcttcca gatacccttc
     7861 tgacattgac gcattaccat ggctgcaacg atcgttaatg gcataaaact cgccgccaac
     7921 gttgaacagg gcgatgacgg gcgaggtatc gatccggaga gcctcaccct ccggcacatc
     7981 cgcaacggga cacgcataaa ttcgattcat cagaacagta cactcaggtt atgggaagtg
     8041 attaccgctt gatccaggac gatatcccgt tccagcaagc gccagttgtc atcttccaga
     8101 cggcgaactt tgtcaaaacg agtcccgaca tagaacgttt catcgcgctc tttttgtgcc
     8161 cgataaagca gataattcac ccgaacagca aatacgtttg ggatgtcggt ttcgcttatc
     8221 tggcagttgc tgattaagtg gcgggtgcgt gacggcggct cttctgccca tgccatgccc
     8281 gtttccagac gggcgattcg ccgctccagc tggtctttgg tgtcattaaa aatccaggtt
     8341 gtcggtggct ggacgccttt gcggcggtcg cgtgtttgcg cgttaactgt ggtgcgcatg
     8401 gtgtaacgaa tctcttcgtc gagctgcgcc agccagtcac gaaatttcca gtcgtccagt
     8461 aagctggctt cgtgaaagag aaactggcta atgcggtgat gtaactctag tgaaacttgc
     8521 gcactcattt catcacctcc tgctggtaag cggcggtttt atcgagcact tcctgccagc
     8581 tttcgctact cagaagatcg gcccagcgtt ggtacattcc acgagcggca gtttctgaaa
     8641 agatatagtt agtaatgcca ggaatgccgt cgtcgcggcg cttttcctga ccaagcccca
     8701 tttccagaca cagtttgctg ttgcgggcgc ggtggccttt aagcaatttc tggatttcac
     8761 accagttctc cgagtcatcc tgctcgagaa aaccagcagg accaaaagca cgagtggcgc
     8821 tgttttcaaa agcggcttta acttcatcgg aggcggcttt gtcagtaata cagaacgccc
     8881 acacttcaac ttgatcaggg ccgcgcggat gccagacgcg gagtgtggca gtgccgttta
     8941 gccatgaaag cgtggggaat atattgttat gacccgccag gcgcagggcg cgaacttcac
     9001 ctaaacgttg ttctgcttcg gcataggttt cgcggtaata gcttgaaact gcgccatcga
     9061 cccagacatt agcatccggt ttttcagtaa agaagaaacc gctaccgtga ccgtcctgac
     9121 caaattgcag cgcatctttg gcggtttccc acaccgggcg ggcggtttgt ccatcaccga
     9181 ggcgcttatc gctgccatca tcttttgccc ctaatacctg aacggcagaa gcatggctga
     9241 acagagcatg atactggtca ctggcgaact gctctgccgg gaatttccag ttacagttga
     9301 tcacccactt ttgtacgccg ccgacaattt cggtgccgcc ttcgcgacga tccagcatgc
     9361 catccagata ccaggcaatg tcacccaggt aatcacgcag gcccggtgcg ctggtatccc
     9421 agttgccaaa aatcagccct ttataactct ccacacaagg aacttcgttt agtccccagt
     9481 gggatttaca caacccttgt gggtaggcgc gaggttccag cggtacatcg atcaactcgc
     9541 cgttaatgcc ataagaccag ccgtgatacg ggcaggtaaa ggcgcgagtg ttgccgcaat
     9601 ctgcataact cacacgcatg gcccggtggc ggcattggtt gagaaacgcc ttgatgctgc
     9661 cgtctttctg acgcactacg acaaccgcat cttctcccat gtaggtgtta aagaaatcac
     9721 cgggttttgg gatctggctt tcgtgggcga gaaatagcca gcaacgaccg aaaatacgct
     9781 caagctccag ttggtaaata tctgggtcgg tataaatacg cggagtgacc cgaccatttt
     9841 gggtgtcaat cagttggtaa atgttcaaat ctgagggtgt ggtcatattt tttaatcctg
     9901 aaagataaga tgtattgacc ttctattttt gtgccataag gatgttgctg gttggttgct
     9961 aatatgtgaa atagttaatt tctaccagct gagacgtttt ttcgataaca aggggaaggt
    10021 gatggaacta cggcatttac gctatttcgt cgcagtggcg caggcactga actttacccg
    10081 tgcggcggaa aaactgcata cctcacagcc ttcgttaagc agccagatcc gcgatcttga
    10141 aaactgtgtc ggtgttccgt tactggtgag ggataagcgc aaagtcgcgt tgacggcggc
    10201 gggagaatgt tttctccagg acgcgctggc aatcctcgaa caagcggaaa atgccaaatt
    10261 acgggcgcgg aaaattgttc aggaagacag acaattaacc attggctttg tgccatcggc
    10321 ggaagtgaat ttactgccaa aagtattacc gatgtttcgt ctcagacagc cagacacctt
    10381 gattgagctg gtgagtttaa tcaccacgca acaggaggaa aaaattcgcc gtggtgaact
    10441 cgacgtcggc ttaatgcgcc atcctgttta tagcccggag attgattatc tggagctttt
    10501 tgacgaacca ttagtggttg tgttaccggt tgatcaccct ttagcacatg aaaaagagat
    10561 caccgccgcg caactggatg gcgtgaattt cgtcagtacc gatccggcgt attccggttc
    10621 gcttgcgccg atcgttaaag cgtggtttgc gcaagaaaac agccagccaa atatcgtcca
    10681 ggtggcaacg aatattctgg tgaccatgaa tctggtgggc atggggctgg gcgtcacttt
    10741 gatacccggt tatatgaata attttaatac cgggcaggtt gtttttcgtc ccattgccgg
    10801 aaatgtgcct tccattgctt tactgatggc gtggaagaaa ggggagatga agccggcatt
    10861 acgcgatttc atcgccattg tgcaggaacg tttggcaagc gtaacggcat aattacgcat
    10921 tatttttgca acatgcgtcg cggatcgcag ttttttagtg cttgtgaact ggcgttacag
    10981 acatcgggaa gcaggctcga atattcttat ttttcaactt gcaccaacat tcgattaacc
    11041 ccagactaac gcctcctgac gggagggact catggttttg caatccacgc gctggttggc
    11101 gctcggctat ttcacatact tttttagtta cggcattttt ctacctttct ggagcgtctg
    11161 gcttaaaggg attggtttaa cgccagaaac catcggcctg ttattggggg caggtctggt
    11221 tgcccgtttc ctcgggagtt tgctcatcgc gccccgcgtc agcgatcctt cccgcctgat
    11281 ttccgccttg cgcgtgctgg cactgctgac acttctcttt gctgtcgcct tctgggcggg
    11341 ggcgcacgta gcgtggctga tgctggtgat gattggcttt aacctctttt tctcaccact
    11401 ggtaccgttg accgatgcac tggcgaatac gtggcaaaag cagttcccgc ttgattacgg
    11461 caaagtgcga ctgtggggct cggtggcgtt tgtcattggc tcggcgctga cgggcaaact
    11521 ggtcactatg tttgattatc gggtgatcct cgcgctgttg acgttgggcg tggcatccat
    11581 gctgctcggc tttctcatcc gtccgacgat tcagccacaa ggggcaagtc gccagcagga
    11641 gagcaccggt tggtctgcgt ggttggcgct ggttcgccag aactggcgct ttctggcctg
    11701 cgtttgttta ttgcaggggg cacatgcggc ctattacggt tttagcgcca tttactggca
    11761 ggctgctggc tactcggcct cggcggtggg gtatttgtgg tcgctgggcg tggtggcgga
    11821 agtcattatc tttgcgctga gtaataaact tttccgccgt tgtagtgcac gcgatatgct
    11881 gttgatctcg gcgatttgcg gcgtagtgcg ctggggcatt atgggagcaa ctacggcgtt
    11941 gccgtggttg atagtggtgc aaattctgca ttgcggcacc ttcacggtct gccacctggc
    12001 cgccatgcgt tatattgctg ctcgccaggg tagcgaagtc atccgtttac aggcggttta
    12061 ctctgccgtc gcgatgggcg gcagtatcgc tatcatgacc gttttcgccg gtttcctgta
    12121 tcaatatctg ggccacggcg tgttctgggt aatggcgctg gtggcgctgc cggcaatgtt
    12181 tttgcgcccg aaagttgttc cctcatgctg attcgagcat tttgcggatc tgctcctgtt
    12241 gatgtgttgt cagcgtcagg tccgtataga tcagcggtgg tgaaaagagc ggtaatggca
    12301 tggtataagg cgcaataatc agtgccgcgc cacgcggagc gcctgtctgc aaaaatgcct
    12361 ttaccgacat atgcttaatg ttgagcggca gtaacgttag ttcgcgtaac tgctgctcaa
    12421 tctgcgcttc tcgctcgcta tcattcccgg tcagtagaat aatctgtttt tcatgcagat
    12481 cgttttcctg cattagccag gcaccgaaaa tcaccgcgac cagaccactt tcctcatcag
    12541 aaagatggac gccgtattca ctttcaaatc cggccagcgc cgcgcgggtg gtgcgcacca
    12601 ggcgtgggta cagtctggcg aactcttccg gcagggtatt atcaataccg atggcaaaca
    12661 aactgcgatt taacgcctgg gcgaggtggg tataaagctg atcacataac ccctgttcat
    12721 cataaaaacg aacatttccc agctcacgaa aatggtttac cagacgtttg atagactgac
    12781 gcaattgtct gtctcgctga tgcgcatccc gcagtggatc gggaacccgc aacatggaaa
    12841 aaagtagtgc cataaacagt ggctcatcag gcggtacagg ctggagcgcc cgacgctgcc
    12901 agtggcgtcc aatttcctgc gctacctgaa attcaaggca ggattccgcc cagcgacgtt
    12961 ggagcggatt aaactgaggc gtaattccag cgtggtgttg cagcaaacaa tattgcagat
    13021 acagacacag gaaatgaata tcgcgcgttt caaagcgttt ttgcagtcgt cgggagcaga
    13081 gattcactaa cgcttgtaga ttggtatcgt cgtaaaagtt acgcgcaatt ccgcgccgct
    13141 tcagttcact cttcagggcg ggggtgaaat ggctggtaat aaatgatgga cacagacgga
    13201 aaccacgtcg tagccagtga aataaacaca aacgttggtt aagcactgta ccttcaaccc
    13261 ggtagctacc gtcataacca gccgtcagtg tgagttgatg atagcgcagg atctcctgtc
    13321 ctgtttcgct gatatcaaga ctggcaatat catcatccac accattaagc tcgctgaaag
    13381 ttgccgtggt ggcggtcaac cccggctgaa agagcgtcag caagatctgg cagcggcgct
    13441 ggggagccga aaggacagat ggtggagcaa gcgtaggcat catctgtaga tctcccataa
    13501 gcataattga taagaatagc taaagtttgc tcagggaaat ggcgaaggga atgctaatca
    13561 tcagaaatgt tggcaagcgt cacagaattt ttctcatctt aaactgttct gaccagtgga
    13621 ttagcatgtc ttaaggtggt tttgtgaatg attatcgtgc tagcgatcat gggttagttc
    13681 tgtggattca acgcgctaag gataaagtcc accatctgtt cgcgataaac atcgccgtga
    13741 cgatcggaaa aagcatcaat gtgttcgcca tccgggatta ggatcaaccg ttttggctct
    13801 tttgccaggc tatacaactt ttcgctgtgc tgccatggga taacgtgatc agctttaccg
    13861 tgaatgagta aaagcgggat cgggctgacg ctggcgatat aattttcgcc gctgtaactc
    13921 tcatcaagta agtagccact gccggggatc atctggttgg cgatggttgc ataagaggca
    13981 aatgtggagt cgaggatcac cgcacgtatg ccttcacgat caccccgacc aataacatcc
    14041 agaatattcg ccccgccaat actctgcccg aacagcacca gacgttgtgg gtttacatca
    14101 ctgcgatggc gcaccacatt gatggcactt tgcgtatcgt ccagcaatcc ggcttgggac
    14161 ggcgtgcctt ttgatttacc aaacccgcga taatcaaaca taaaaacgtt gaaattacgc
    14221 tcaggtaacc aactgaccag cggccagtgg gcggacatat ttccggcatt gccgtgagca
    14281 tgaatgatgg ttgcgatggc gttgtcagca gggcccgtcg aagaaggaat aaaccagcct
    14341 tgcagacgag taccgtcctt agccgtaaat tcgacggatt ccgccgacca ggggtcggga
    14401 ccgtaaattt tatcgtcagg ataatagaag aagttgatgg caacgcgcgg cactaaataa
    14461 acgcagaagg caacaacaaa gagaataaac agaattttgg gaacgcgttt gttcactggc
    14521 agtgccataa cctttccttg cgacttcttt atcatccatt aatcggggaa agatggtggg
    14581 agagtgacga aagaaagtaa agcggtgagg aagtatttca gaatagcgcc tgaaaggcga
    14641 gggcgggtga gtgacatcac ccgcctgagt cattaacgct tcagagcgtc gcttaactcg
    14701 tcacgcatgt tcgccagcat ggctttcaca acacgtgggt taccagcaac gatgttaccg
    14761 gtcagcatat agttatgacc accggtgaag tcgctgacga tgccgcccgc ttcacgaacc
    14821 agcagctcgc ctgccgcgaa atcccacgga cgcagaccga tttcaaagaa accgtcaaca
    14881 cgacccgcag cgacgtaagc cagatccagc gccgcagaac cggtacgacg gaagtctgca
    14941 cattcgttga acagtttgcc gacgatgttg atgtaggtag tggcgtactg ttttgctttg
    15001 aacgggaagc cggtcgccag aatggtaccg tcgagatcgc gagcggtgct accgcgcaga
    15061 cggtagccgt tcagctgtgc gccctgaccg cgagtggcgg tgaacagttc gttacgcata
    15121 ggatcgtata ccacagcaac ttcggtgcgg cctttgatac gaacggcgat agataccgcg
    15181 aagtgcggca gacgtttgat aaagttggta gtaccatcca gtggatcgat aacccattga
    15241 acatcctgat cagtaccttc aagttcaccg ctttcttcgg tgatgatggt gtgctgtggg
    15301 taagatttac gaatcgtgtc gataatcacc gcttcggcag ctttatctac gttggtcacg
    15361 aaatcgttac tgcctttctg gctcgcttct acagcgtccg gggtttcata gtttttggca
    15421 attaaattac ccgccttgcg cgctgcgcgc acggcgatgt tcagcatcgg atgcatcggt
    15481 ctctctcact ggatgttaaa gaacgggaaa acggcgcaga gtatagcagc gagatcggca
    15541 tctgtcttcg ttttatgata agatgcgtga ataatcttta gatgaagaaa gacaatgctg
    15601 caaaatattc gaattgtgct ggtggagacg tcacacaccg gcaatatggg ttctgttgcc
    15661 cgtgccatga aaacaatggg attaaccaat ctgtggctgg ttaatccact ggtgaaaccc
    15721 gactcccagg cgattgccct ggcagcaggg gccagcgatg tgattggtaa tgctcacatc
    15781 gtcgatacgc tcgacgaagc gttagccggt tgtagcctgg tggtgggcac cagcgcacgc
    15841 tcccgcacgc tgccgtggcc gatgctcgac ccgcgcgaat gcggtctgaa aagcgtcgct
    15901 gaagcggcaa atacccctgt ggcgctggtg tttggtcgtg agcgcgtcgg cttgaccaat
    15961 gaagagttgc agaaatgcca ttatcatgtc gcgattgcgg ctaacccgga atacagctcg
    16021 ctgaacctgg cgatggcggt tcaggttatt gcctatgaag tgcgcatggc ctggctggca
    16081 actcaggaaa acggcgagca ggtcgagcat gaagagacgc cgtatccgct ggtcgatgat
    16141 ctggagcgtt tttatggtca tctggaacaa acgctgctgg caaccggttt tatccgtgaa
    16201 aaccatccgg ggcaggtgat gaataaattg cgccgtctgt ttacccgtgc gcgcccggaa
    16261 agccaggaat tgaatatcct gcgcgggatt ctggcttcta ttgagcagca gaataaaggt
    16321 aataaggcct gagggctaag ccagataaat gcacggacgg tcccctcgcc ccttcgggga
    16381 gagggttagg gtgaggggaa gaggccagcg ctaatgcgaa taattgccct caacccgccc
    16441 atggtctgga aggacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa
    16501 tacccgacta aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga
    16561 ccctgctatg caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag
    16621 gtgaagtaag acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac
    16681 gttgcgctca actctgaagc gggcccggta ccgttggctg atatttccga acgtcaggga
    16741 atttcccttt cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc
    16801 agcgtacgtg gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt
    16861 ggcgaagtga ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc
    16921 ggctgccagg gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt
    16981 ctcaccggtt ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg
    17041 gatgtgtctg gtcgtcagca tactcacgac gcgccacgca cccgcataca agacgcgatc
    17101 gacgttaagt tacgcgctta ataaaaagaa ttcagaatca ggccggggcg tatcgcgctc
    17161 cgtaaacacg gtcgtacatc cagccggtag cctggttcct tgcattgagt gatgtacgga
    17221 gtttatagag caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac
    17281 ccgcgtgttg ccgagaaaat gatgcagttt atgacgatgg acggaacctt tggtaacccg
    17341 gcctctcgtt ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat
    17401 cagattgccg atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc
    17461 gaatctgaca acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac
    17521 atcatcacca gcaaaaccga acacaaagcg gtactggata cctgccgtca gctggagcgc
    17581 gaaggttttg aagtcaccta cctggcaccg cagcgtaacg gcattatcga cctgaaagaa
    17641 cttgaagcag cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa
    17701 atcggcgtgg tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc
    17761 tatcacgttg atgcaaccca gagcgtgggc aaactgccta tcgacctgag ccagttgaaa
    17821 gttgacctga tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg
    17881 tatgtacgtc gtaaaccgcg cgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag
    17941 cgcggtatgc gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaggcctat
    18001 cgcatcgcaa aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt
    18061 ctgtggaacg gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt
    18121 gcgccgaaca ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg
    18181 ctgaaagacc tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc
    18241 tacgtgctgc gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct
    18301 ttaggtcgtt ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc
    18361 atcggtcgtc tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg
    18421 aacagcatcg aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct
    18481 tacagcgaaa aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgacaac
    18541 aacgacgaga acgtcggcag cggcatggtg ggggcaccgg cctgtggcga cgtgatgaag
    18601 ttgcagatta aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc
    18661 tgcggttctg ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc
    18721 gacgaagcgc aggcgatcaa aaataccgat attgctgaag aacttgaact gccgccggtg
    18781 aaaattcact gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa
    18841 agcaaacgtg aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag
    18901 tgcagcagcg cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct
    18961 gggcgtgaga acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc
    19021 gacgccggaa gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag
    19081 cctgcaattt ctggacggta cgcagctgga cttcgtaaaa gaaggcctga acgaagggtt
    19141 taaattcacc aacccgaacg tcaaagatga gtgtggttgc ggcgaaagct tccacgtttg
    19201 atgcgcatac cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc
    19261 gccgttatcg cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc
    19321 ttgcctgccc gttatcaact cgatacccag gcgctgagcc tgcgttttca ggatctacaa
    19381 cgtcagtatc atcctgataa attcgccagc ggaagccagg cggaacaact cgccgccgta
    19441 cagcaatctg caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg
    19501 gaatatttgc tttctttaca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac
    19561 accgcgttcc tgatggaaca gttggagctg cgcgaagagc tggacgagat cgaacaggcg
    19621 aaagatgaag cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc
    19681 catcagttga tggttgaaca gttagacaac gaggcgtggg acgcggcggc ggataccgtg
    19741 cgtaagctgc gttttctcga taaactgcgg agcagtgccg aacaactcga agaaaaactg
    19801 ctcgattttt aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt
    19861 gagtgctgcg ccgcatcagc gtcgtctggc ggctggtatt gacctgggca caaccaactc
    19921 gctggtggcg acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca
    19981 cctgctgcca tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg
    20041 tactaacgca gcgcttgata ccgccaacac cattagttct gttaaacgcc tgatggggcg
    20101 ctcgctggct gatatccagc aacgctatcc gcatctgcct tatcaattcc aggccagcga
    20161 aaacggcctg ccgatgattg aaacggcggc ggggctgctg aacccggtgc gcgtttctgc
    20221 ggacatcctc aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg
    20281 tgtagttatc accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc
    20341 ggcgcgtctg gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat
    20401 cgcctacggg ctggattccg gtcaggaagg cgtgatcgct gtttatgacc tcggtggcgg
    20461 gacgtttgat atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg
    20521 cggtgattcc gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga
    20581 gcaggcgggc attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc
    20641 cattgcagcc aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg
    20701 gcagggcgaa atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac
    20761 cttactggct tgtcgtcgcg cgctgaaaga cgcgggtgta gaagctgatg aagtgctgga
    20821 agtggtgatg gtgggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt
    20881 tttcggtcgt ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc
    20941 gattcaggcg gatattctgg tgggtaacaa gccggacagc gaaatgctgc tgcttgatgt
    21001 gatcccactg tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg
    21061 taataccacg attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac
    21121 ggcgatgtct atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact
    21181 ggcgcgtttt gcgctgcgtg gtattccggc gctaccggct ggtggtgcgc atattcgcgt
    21241 gacgttccag gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg
    21301 cgttgaggcg tctattcagg tcaaaccgtc ttacggtctg accgatagcg aaatcgcttc
    21361 gatgatcaaa gactcaatga gctatgccga gcaggacgta aaagcccgaa tgctggcaga
    21421 acaaaaagta gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc
    21481 cgcgctgtta agcgccgcag aacgtcaggt cattgacgat gctgccgctc acctgagtga
    21541 agtggcgcag ggcgatgacg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca
    21601 aacccaggat ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca
    21661 ttccgtggac gaggtttaat atgccaaaga ttgttatttt gcctcatcag gatctctgcc
    21721 ctgatggcgc tgttctggaa gctaatagcg gtgaaaccat tctcgacgca gctctgcgta
    21781 acggtatcga gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca
    21841 tcgttcgtga aggttttgac tcgctgccgg aaagctcaga gcaggaagac gacatgctgg
    21901 acaaagcctg gggactggag ccggaaagcc gtttaagctg ccaggcgcgc gtcaccgacg
    21961 aagatttagt ggtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga
    22021 ggttagtatg ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc
    22081 gtatcccgat cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga
    22141 tttggaagat ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt
    22201 gttagtctgg ctggacgagg ccgaataatt tccgcgtcat gcttcacgcc gcagatgcgt
    22261 tggctgcgct cgttcacccc agtcacatag ttatctatgc tcctggggct tcactcactt
    22321 gccgccttcc tgcaacgcga atcatttagc ggaaaaatcc tggggctgcc aactggcggc
    22381 ccttttacaa agaaggataa ctaaaatgac agaagcgatg aagattaccc tctctaccca
    22441 acctgccgac gcgcgctggg gagaaaaagc aacttacagc attaataatg acggcattac
    22501 cctgcatttg aacggggcag acgatctggg gctgatccag cgtgcggcgc gcaagattga
    22561 cggtctgggc atcaagcatg ttcagttaag cggtgaaggc tgggatgcgg atcgctgctg
    22621 ggcattctgg caaggttaca aagccccgaa aggcacgcgt aaagtggagt ggccggatct
    22681 ggacgatgcc cagcgccagg aactggataa ccgcctgatg atcatcgact gggtgcgtga
    22741 caccatcaac gcaccggcag aagaattggg accatcgcaa ctggcacagc gtgctgttga
    22801 tctgatcagc aacgtcgcgg gcgatcgtgt gacttatcgg atcaccaaag gcgaagatct
    22861 gcgtgagcaa ggttatatgg ggctgcacac tgtcgggcgc ggttcagaac gttctccggt
    22921 attgctggcg ctggattaca acccaactgg cgataaagaa gcaccggtgt acgcgtgcct
    22981 ggtaggtaaa ggtatcactt ttgactccgg cggctacagc ataaaacaga ctgcgtttat
    23041 ggactcgatg aagtcggaca tgggcggcgc ggcaacggtt accggggcgc tggcatttgc
    23101 cattacgcgc ggactgaaca agcgcgtgaa gctgttcctc tgctgtgcgg ataacctgat
    23161 tagcggcaat gcgttcaagc tgggcgatat catcacctat cgcaacggta aaaaagttga
    23221 agtgatgaac actgatgccg aagggcgtct ggtgctggcc gatggtctga ttgatgccag
    23281 tgcgcagaaa ccggaactga tcattgatgc ggcgaccctc accggggcgg cgaaaactgc
    23341 gctgggtaat gattatcacg cgctgttcag ttttgacgat gcgcttgccg gtcgtttgct
    23401 ggcgagtgcc tcacaagaga acgaaccgtt ctggcgtctg ccgctggcgg aattccaccg
    23461 cagccagctg ccgtctaact ttgccgaact gaacaatacc ggaagcgcgg cgtatccggc
    23521 aggcgcgagc acggcggcgg gcttcctgtc gcactttgtt gagaactatc agcaaggctg
    23581 gctgcatatc gactgctcgg cgacttaccg taaagcgccg gttgaacagt ggtctgcggg
    23641 cgctacggga cttggtgtgc gcacgattgc taatctgtta acggcgtaaa ctaatatgcc
    23701 ggatgcggcg taaacgcctt atccggccta caaattctgc aaattcaaca aattgcgaat
    23761 cccttgtagg cctgataagc gtagcgcatc aggcatttgc gccccgaacg ttgaatgaat
    23821 tgtggttctt atgtccgaaa caaaaaacga acttgaagac ctgctggaaa aagcagcaac
    23881 cgaaccggcg caccgcccgg cctttttccg tactctactg gaatccaccg tctgggtgcc
    23941 tggtacggcg gcgcagggcg aggctgtggt tgaagatagt gcgcttgatt tacagcactg
    24001 ggaaaaagaa gacggcacca gcgtcattcc ttttttcacc tcgttagaag cgcttcaaca
    24061 ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt cgcacattgt ttgagatgac
    24121 ccttggcgaa acgctcttcc ttaatgccaa actgccgacc ggtaaagaat ttatgccgcg
    24181 tgaaatcagt ttgttgattg gtgaagaggg aaatccgctg agcagccagg aaatcctgga
    24241 aggcggtgaa tcgctgatat tatcggaagt cgcagagccg ccagcacaaa tgattgattc
    24301 actcaccacc ttatttaaaa ccattaagtc ggtgaagcgt gcttttattt gttcaattaa
    24361 agagaacgaa gaggcacagc ctaatttact tattggcatt gaagccgatg gtgatatcga
    24421 agaaattatt caggcgacgg gaagtgtagc gaccgataca ttacctggcg atgaaccaat
    24481 cgatatttgt caggtgaaaa aaggggaaaa aggaattagc cactttatta ccgaacatat
    24541 tgtgccattc tatgaacgtc gctggggtgg ttttttgcgt gactttaaac agaatcggat
    24601 aatctaaaat atgccgcccc aaagggcggc attcgggctt acttgctgct ctgaaggttg
    24661 ttaaccacc
//
LOCUS       DAZFVO020000065        24484 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFVO020000065 DAZFVO020000000
VERSION     DAZFVO020000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.065"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..462
                     /locus_tag="ACHKNB_RS12205"
                     /old_locus_tag="pgaptmp_002441"
     CDS             <1..462
                     /locus_tag="ACHKNB_RS12205"
                     /old_locus_tag="pgaptmp_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4195937.1"
                     /translation="AINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEI
                     AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEA
                     TDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            618..2153
                     /gene="gadC"
                     /locus_tag="ACHKNB_RS12210"
                     /old_locus_tag="pgaptmp_002442"
     CDS             618..2153
                     /gene="gadC"
                     /locus_tag="ACHKNB_RS12210"
                     /old_locus_tag="pgaptmp_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HGU4195938.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2284..3603
                     /locus_tag="ACHKNB_RS12215"
                     /old_locus_tag="pgaptmp_002443"
     CDS             2284..3603
                     /locus_tag="ACHKNB_RS12215"
                     /old_locus_tag="pgaptmp_002443"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HGU4195939.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGIIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3980..5362
                     /gene="dosC"
                     /locus_tag="ACHKNB_RS12220"
                     /old_locus_tag="pgaptmp_002444"
     CDS             3980..5362
                     /gene="dosC"
                     /locus_tag="ACHKNB_RS12220"
                     /old_locus_tag="pgaptmp_002444"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HGU4195940.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5387..7776
                     /gene="dosP"
                     /locus_tag="ACHKNB_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /gene_synonym="pdeO"
                     /pseudo
     CDS             5387..7776
                     /gene="dosP"
                     /locus_tag="ACHKNB_RS12225"
                     /old_locus_tag="pgaptmp_002445"
                     /gene_synonym="pdeO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
     gene            8035..8616
                     /gene="ddpX"
                     /locus_tag="ACHKNB_RS12230"
                     /old_locus_tag="pgaptmp_002446"
     CDS             8035..8616
                     /gene="ddpX"
                     /locus_tag="ACHKNB_RS12230"
                     /old_locus_tag="pgaptmp_002446"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HGU4195941.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIY
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8630..10180
                     /locus_tag="ACHKNB_RS12235"
                     /old_locus_tag="pgaptmp_002447"
     CDS             8630..10180
                     /locus_tag="ACHKNB_RS12235"
                     /old_locus_tag="pgaptmp_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4195942.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10182..11204
                     /locus_tag="ACHKNB_RS12240"
                     /old_locus_tag="pgaptmp_002448"
     CDS             10182..11204
                     /locus_tag="ACHKNB_RS12240"
                     /old_locus_tag="pgaptmp_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4195943.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11201..12097
                     /gene="ddpC"
                     /locus_tag="ACHKNB_RS12245"
                     /old_locus_tag="pgaptmp_002449"
     CDS             11201..12097
                     /gene="ddpC"
                     /locus_tag="ACHKNB_RS12245"
                     /old_locus_tag="pgaptmp_002449"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HGU4195944.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12094..13080
                     /locus_tag="ACHKNB_RS12250"
                     /old_locus_tag="pgaptmp_002450"
     CDS             12094..13080
                     /locus_tag="ACHKNB_RS12250"
                     /old_locus_tag="pgaptmp_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4195945.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13073..13999
                     /locus_tag="ACHKNB_RS12255"
                     /old_locus_tag="pgaptmp_002451"
     CDS             13073..13999
                     /locus_tag="ACHKNB_RS12255"
                     /old_locus_tag="pgaptmp_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HGU4195946.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14055..14486)
                     /gene="osmC"
                     /locus_tag="ACHKNB_RS12260"
                     /old_locus_tag="pgaptmp_002452"
     CDS             complement(14055..14486)
                     /gene="osmC"
                     /locus_tag="ACHKNB_RS12260"
                     /old_locus_tag="pgaptmp_002452"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HGU4195947.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14832..15047
                     /gene="bdm"
                     /locus_tag="ACHKNB_RS12265"
                     /old_locus_tag="pgaptmp_002453"
     CDS             14832..15047
                     /gene="bdm"
                     /locus_tag="ACHKNB_RS12265"
                     /old_locus_tag="pgaptmp_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HGU4195948.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15149..15286
                     /gene="sra"
                     /locus_tag="ACHKNB_RS12270"
                     /old_locus_tag="pgaptmp_002454"
     CDS             15149..15286
                     /gene="sra"
                     /locus_tag="ACHKNB_RS12270"
                     /old_locus_tag="pgaptmp_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HGU4195949.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15443..17140
                     /gene="maeA"
                     /locus_tag="ACHKNB_RS12275"
                     /old_locus_tag="pgaptmp_002455"
     CDS             15443..17140
                     /gene="maeA"
                     /locus_tag="ACHKNB_RS12275"
                     /old_locus_tag="pgaptmp_002455"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HGU4195950.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17274..18284
                     /gene="adhP"
                     /locus_tag="ACHKNB_RS12280"
                     /old_locus_tag="pgaptmp_002456"
     CDS             17274..18284
                     /gene="adhP"
                     /locus_tag="ACHKNB_RS12280"
                     /old_locus_tag="pgaptmp_002456"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGU4195951.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18431..18715
                     /locus_tag="ACHKNB_RS12285"
                     /old_locus_tag="pgaptmp_002457"
     CDS             18431..18715
                     /locus_tag="ACHKNB_RS12285"
                     /old_locus_tag="pgaptmp_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HGU4195952.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18766..19419)
                     /gene="fdnI"
                     /locus_tag="ACHKNB_RS12290"
                     /old_locus_tag="pgaptmp_002458"
     CDS             complement(18766..19419)
                     /gene="fdnI"
                     /locus_tag="ACHKNB_RS12290"
                     /old_locus_tag="pgaptmp_002458"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HGU4195953.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19412..20296)
                     /gene="fdnH"
                     /locus_tag="ACHKNB_RS12295"
                     /old_locus_tag="pgaptmp_002459"
     CDS             complement(19412..20296)
                     /gene="fdnH"
                     /locus_tag="ACHKNB_RS12295"
                     /old_locus_tag="pgaptmp_002459"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HGU4195954.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20309..23356)
                     /gene="fdnG"
                     /locus_tag="ACHKNB_RS12300"
                     /old_locus_tag="pgaptmp_002460"
     CDS             complement(20309..23356)
                     /gene="fdnG"
                     /locus_tag="ACHKNB_RS12300"
                     /old_locus_tag="pgaptmp_002460"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22769..22771),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HGU4195955.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23588..24469
                     /gene="yddG"
                     /locus_tag="ACHKNB_RS12305"
                     /old_locus_tag="pgaptmp_002461"
     CDS             23588..24469
                     /gene="yddG"
                     /locus_tag="ACHKNB_RS12305"
                     /old_locus_tag="pgaptmp_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HGU4195956.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5974 a         6221 c         6211 g         6078 t
ORIGIN      
        1 gccatcaact tctcccgccc ggcgggtcag gtaattgcac agtactatga attcctgcgc
       61 ctcggtcgtg aaggctacac caaagtacag aacgcctctt accaggttgc cgcttatctg
      121 gcggatgaaa tcgccaaact ggggccgtat gagttcatct gtacgggtcg cccggacgaa
      181 ggcatcccgg cggtttgctt caaactgaaa gatggtgaag atccgggata caccctgtac
      241 gacctctctg aacgtctgcg tctgcgcggt tggcaggttc cggccttcac tctcggcggt
      301 gaagccaccg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      361 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      421 ctgcagggta ttgcccagca gaacagcttt aaacatacct gataacgttt aacggtaacg
      481 gtgtcccgaa acgaacccgt ttcgggacaa tttccaaagt ctgttcactg gcattagcaa
      541 cggaaaatat tgttctgaat acgcttcaga acaaaacagg tgcggttccg acaggaatac
      601 cgttttaggg ggataatatg gctacatcag tacagacagg taaagctaag cagctcacat
      661 tactcggatt ctttgccata acggcatcga tggtaatggc tgtttatgaa taccctacct
      721 tcgcaacatc gggcttttca ttagtcttct tcctgctatt aggcgggatt ttatggttta
      781 ttcccgtggg actttgtgct gcggaaatgg ccaccgtcga cggctgggaa gaaggtggtg
      841 tcttcgcctg ggtatcaaat actctggggc cgagatgggg atttgcagcg atctcatttg
      901 gctatctgca aatcgccatt ggttttattc cgatgctcta tttcgtgtta ggggcactct
      961 cctacatcct gaaatggcca gcgctgaatg aagaccccat taccaaaact attgcagcac
     1021 tcatcattct ttgggcgctg gcattaacgc agtttggtgg cacgaaatac acggcgcgaa
     1081 ttgctaaagt tggcttcttc gccggtatcc tgttacctgc atttattttg atcgcattag
     1141 cggctattta tctgcactcc ggtgcccccg ttgctatcga aatggattcg aagaccttct
     1201 tccctgactt ctctaaagtg ggcacactgg ttgtatttgt tgccttcatt ttgagttata
     1261 tgggcgtaga agcttccgca actcacgtca atgaaatgag caaccccggg cgcgactatc
     1321 cactggctat gttactgctg atggtggcgg caatctgctt aagctctgtt ggcggtttat
     1381 ctattgcgat ggtcattccg ggtaatgaaa tcaacctctc cgcaggtgta atgcaaacct
     1441 ttaccgttct gatgtcccat gtggcaccgg aaattgagtg gacggttcgt gtgatctccg
     1501 cactgctgtt gctgggtgtt ctggcggaaa tcgcctcctg gattgttggt ccttctcgcg
     1561 ggatgtatgt cacagcgcag aaaaacctgc tgccagcggc attcgctaaa atgaacaaaa
     1621 atggcgtacc ggtaacgctg gtcatttcgc agctggtgat tacttctatc gcgttgatca
     1681 tcctcaccaa taccggtggc ggtaacaaca tgtccttcct gatcgcactg gcgctgacgg
     1741 tggtgattta tctgtgtgct tatttcatgc tgtttattgg ctacattgtg ttggttctta
     1801 aacatcctga cttaaaacgc acatttaata tccctggtgg taaaggggtg aaattggtcg
     1861 tggcaattgt cggtctgctg acttcaatta tggcgtttat tgtttccttc ctgccgccgg
     1921 ataacatcca gggtgattct accgatatgt atgttgaatt actggttgtt agtttcctgg
     1981 tggtacttgc cctgcccttt attctctatg ctgttcatga tcgtaaaggc aaagcgaata
     2041 ccggcgtcac tctggagcca atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc
     2101 acccgcgtgc acgttcacca cactatattg tgatgaatga caagaaacac taaccatgtc
     2161 tgaatggtta taagacaagg gagcgataag tcatcgctcc ctttttcgtg cttgctgtcg
     2221 tattgactta accgggataa gtacgagaat gagcgcacat ctgtttaccg gaaaccagca
     2281 catatggata tctgctcccg aaacaagaaa ttaacgatta gaagaccagc gatactagtt
     2341 gcactggcac ttttactgtg tagttgtaaa agcacgcctc cagagtccat ggtgacacca
     2401 ccagcaggtt caaagccacc agccacgacg caacaatcgt cacaaccgat gcgtggcatc
     2461 tggctggcca cggtttctcg gctcgactgg ccaccggttt cctcggttaa cattagtaac
     2521 cccaccagcc gggcccgtgt acaacaacag gcgatgatcg acaaactgga tcatctgcaa
     2581 cgtctcggca taaacacggt ctttttccag gtcaagccgg acggtaccgc cctgtggcca
     2641 tcgaaaattt tgccgtggtc cgatcttatg accggtaaga ttggtgaaaa tccgggttac
     2701 gatccgctgc aatttatgct cgacgaagcc cacaagcgtg ggatgaaagt acacgcctgg
     2761 tttaacccct atcgcgtatc ggttaatacg aagcccggta ttatcaggga attgaatagc
     2821 actctgtctc aacaaccggc gagcgtctat gtgcaacacc gtgactggat cagaacgtct
     2881 ggcgatcgct ttgtcctcga cccgggcatc cctgaggttc aggactggat cacatcaata
     2941 gtcgctgaag tggtttcccg ctatccggta gatggcgtgc agtttgacga ctatttctat
     3001 actgaatcac cgggttcacg gctaaatgat aacgaaacgt accgtaaata cggaggcgca
     3061 tttgcgtcaa aagcagactg gcggcgcaac aatactcagc agttaattgc aaaggtatca
     3121 cacaccatta aaagcattaa gccgggagtc gaatttggcg ttagcccggc aggcgtgtgg
     3181 cgtaaccgat cacacgatcc gctcggttcc gatacccgag gcgcggcagc ctatgacgaa
     3241 tcctacgctg acacccgtcg atgggtggaa caaggattgc tggattacat tgctccccaa
     3301 atttactggc cgttctcacg gagtgccgcg cgttatgacg tgttggcaaa atggtgggcg
     3361 gatgtcgtta aaccgaccag gacccgcctg tatatcggta tcgccttcta taaagtgggt
     3421 gaaccttcaa agatagagcc agactggatg attaacggcg gcgtaccgga actgaaaaag
     3481 cagctcgatc ttaacgatgc ggtaccagaa attagcggca ccatcttgtt ccgtgaggac
     3541 tatctgaata aaccgcagac tcaacaagcg gtcagctatc tgcaaagtcg ttggggcagt
     3601 taaactccca tgcttacttt gtaacaagcc ggtgctttac ccaccggcgt gttacacctt
     3661 gtgaaatatc ccactagtaa tcatgcttac ataagtcaaa ttaaccacgc aattaaccac
     3721 acttagttaa acagctataa atccctaata aaaatgtatg aatatgaaaa accatacgat
     3781 attcgctgtc accgaaaaat aattacgatg ggctatactc ttctgaagaa cagtagcgta
     3841 gattgaaact gtcctaagtc gcttatttct tttcagtata tcttcatatt tcaggagaat
     3901 atggtgcagt tagtcgatct cgcacgttgc gtttcgtttt gattccgtct gatttcccag
     3961 ccttataagg gtgtgaatta tggagatgta ttttaaaaga atgaaagatg agtggaccgg
     4021 acttgtcgaa caggcagatc ctctcattcg tgctaaagcc gcagaaattg ccgttgcgca
     4081 tgctcattat ctgagtatcg agttttatcg aattgtccgc atcgacccgc atgccgaaga
     4141 attcttgagt aatgaacaag ttgagcggca gttgaaaagt gcgatggaac gctggattat
     4201 taacgtgctt tctgcccagg ttgacgatgt cgaaaggcta atacaaatcc agcataccgt
     4261 cgcggaagtg catgcccgca taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt
     4321 gctgaaaaaa atcctctatc cggtaatctt ctcttcggat tattccgccg cagaaaaact
     4381 tcaggtctac catttctcga ttaacagtat tgatatcgct atggaagtga tgacccgcgc
     4441 gtttaccttt agtgacagta gtgcctcaaa ggaagatgaa aactatcgta tcttctcgtt
     4501 actggaaaac gccgaagaag aaaaagaacg gcaaatagcc tcaatacttt catgggaaat
     4561 agatattatc tataaagtcc tgctggattc tgatttaggc agcagtttgc ctttaagcca
     4621 ggctgatttt ggcctgtggt ttaaccataa aggtcgacat tattttagcg gcattgccga
     4681 agtaggccat atctcccgtc tgattcagga tttcgacggt attttcaatc aaaccatgcg
     4741 taacaccagg aatttgaata acagaagttt gcgggtgaag tttctattac agataagaaa
     4801 taccgtatcg caaattatta ccttgctgcg tgaattgttt gaagaagtat cgcgccacga
     4861 agtcggtatg gatgtactga cgaaattact taaccgccgt ttcctgccga ctatcttcaa
     4921 acgcgaaatt gcccatgcca accggaccgg tacaccgctg tcagtgctga ttattgacgt
     4981 tgataaattc aaagagatca acgatacgtg gggccataac actggcgatg aaattctgcg
     5041 taaagtctct caggcctttt atgacaacgt ccgcagtagt gattatgttt tccgctacgg
     5101 gggcgatgaa tttatcattg ttttgactga agcttctgaa aacgaaacgt tacgtaccgc
     5161 agaacgtatt cgcagtcggg tggagaaaac caaactgaaa gccgcaaacg gcgaggatat
     5221 tgccctctca ctttccatcg gtgccgccat gtttaatggt catcctgact atgagcgcct
     5281 cattcaaata gccgatgaag ctctgtatat cgccaaaaga cgaggtagaa accgtgttga
     5341 actctggaaa gccagtcttt agatgcgcca ggatgcagag gtaatcatga agctaaccga
     5401 tgcggataat gccgccgatg gcattttttt ccccgccctt gagcaaaata tgatgggtgc
     5461 ggtgttaatt aacgaaaata atgaagtgat gtttttcaac cccgccgcag agaagctctg
     5521 ggggtacaaa cgtgaagaag tcattggcaa taacattgat atgctgattc cgcgggattt
     5581 gcgtcctgcg catcctgaat acattcgtca taaccgtgaa ggcggtaaag cgcgtgtcga
     5641 ggggatgagt cgggagctgc agctggagaa aaaagacggc agtaaaatct ggacccgttt
     5701 tgcgctatcg aaagtgagcg ccgaggggaa agtttattac ctggcgctgg tacgggatgc
     5761 cagcgtagaa atggcgcaaa aagaacagac tcgacaattg attattgccg ttgaccatct
     5821 cgaccgaccg gtgattgtcc tcgatccgga acgccatatt gtgcagtgca atcgcgcatt
     5881 taccgaaatg tttggttact gcattagcga agccagcggt atgcagcccg atacactcct
     5941 gaacattcct gaattccctg ccgataaccg cattcgttta caacagttgc tatggaaaac
     6001 cgcccgcgat caggatgaat ttctgctgtt gacgcgcacc ggtgaaaaaa tctggattaa
     6061 agcctctatc agcccggttt atgacgtgct cgcgcatctg cagaacctgg taatgacctt
     6121 ctcggatatc accgaagaac ggcaaatccg ccagcttgaa ggcaatattc tcgccgccat
     6181 gtgcagcagc ccgccatttc atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt
     6241 tctcaacgaa tcgcatgttt cgctgttcgc actgcgcaac gggatgccga tacactgggc
     6301 gtcatcttcg cacggtgcag aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca
     6361 gcgtgatggc gcgcctgcgg ggatcctgca aattaaaacc tcgtcaggag cagaaaccag
     6421 cgcctttatc gaacgcgtgg cagatatcag tcagcatatg gccgcgctgg cgctggaaca
     6481 ggaaaaaagc cgccagcata ttgaacaact catccaattt gatccgatga ccggtctgcc
     6541 aaatcgcaat aacctgcaca attacctcga tgacctggtc gacaaagccg tctctccggt
     6601 ggtgtatctc atcggtgttg accatattca ggatgtgatt gatagccttg gctatgcgtg
     6661 ggccgatcag gcattgctgg aagtggtcaa tcgctttcgt gaaaaactca aaccggatca
     6721 gtatctctgt cgtatcgaag gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag
     6781 taacattacc caaatcgccg atgagctacg gaatgtggtc agcaagccga taatgattga
     6841 cgataaaccc ttcccgctta ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg
     6901 cgattacctg ctctccactg ctcacaatgc aatggattat attcgcaaga atggcggtaa
     6961 cggctggcag ttcttcagcc cggcgatgaa cgaaatggta aaagagcgtt tggttttagg
     7021 cgcagcgctg aaagaagcga ttagcaataa ccaactgaaa cttgtttacc agccgcaaat
     7081 cttcgcagaa acgggtgaac tgtacggcat cgaagccctt gcgcgctggc acgatcccca
     7141 gcatggtcat gtgccccctt cacggtttat tcctctcgca gaagagattg gtgaaatcga
     7201 aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca
     7261 gaatattcat atcccggcgc tttccgttaa cttgtcggca ctgcactttc gcagtaatca
     7321 actgcctaat caggtgtctg atgcaatgca cgcctggggt attgacggcc ggtagaaatc
     7381 acggaaagca tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgt
     7441 gatatgggcg tgggcttatc ggtagatgat tttggtacgg gcttttccgg attatcccgc
     7501 ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg
     7561 accgaaaaac gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat
     7621 ttaaccgtcg tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc
     7681 cactgtcgcg ttattcaggg atatttcttt tcccgccccc tacccgccga agaaattcca
     7741 ggctggatga gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc
     7801 gggttttcat ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg
     7861 agattccttc cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga
     7921 tcaaaagtta aaaaaatata actttaagcc agcattctgg cgcttatccc ggcatggcat
     7981 gagatctgca taagcggaaa gcgcagcaat ttttgtctta tacaggaacc gtttatgtcg
     8041 gataccaccg aactggttga tttagccgta atcttccctg atctggagat cgaattgaaa
     8101 tacgcctgcg ctgataacat cacaggtaaa gctatttatc agcaagcgcg ttgtctgcta
     8161 cacaaggatg cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta
     8221 caactggtga tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc
     8281 tgcccagacc cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg
     8341 gcgatcgacc tgacgcttcg tgatgagcac gggaacatcc tcgatatggg cgcagggttc
     8401 gatgaaatgc acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc
     8461 aatcggctgt tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctacagcgaa
     8521 tggtggcact tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattcacc
     8581 tgttttatat cccctggcac acaacacgtc agttaatcag gaaactgtca tgaagagatc
     8641 gataccgttt cgtcccacat tgctcgcgct cgtccttgcc acaaatttcc cggttgcgca
     8701 cgccgccgta ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga
     8761 cccggcggta acaatagata ataacgactg gacggtgacc tacccgtctt atcagcggct
     8821 ggttcagtac aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag
     8881 tagctggaaa gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa
     8941 atttgccgat ggcacaccgg tcactgccga agcagtaaaa ctctcttttg agcggctact
     9001 aaaaatcggc caggggccag cagaagcatt tcccaaagat ttaaagattg atgctcccga
     9061 cgaacatacg gtgaagttta cccttagcca gccattcgca ccgttcctct acacgctggc
     9121 gaatgacggt gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc
     9181 tcgcggcttc ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca
     9241 aaaaggtcag caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt
     9301 taagcgagta tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg
     9361 tggcgacatt gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga
     9421 aaataaagtc aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa
     9481 cagcaaagcg cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta
     9541 tcagggcatg gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc
     9601 ggaaggcatg tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc
     9661 caaagccgaa tgggataaag taacgagcaa acccaccagc ctgacgtttc tctactccga
     9721 taacgatccg aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg
     9781 catcaatgtg aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg
     9841 tgattacgac attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat
     9901 gaattactgg tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa
     9961 cagtgaggtc gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac
    10021 ccgggactac cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt
    10081 ccagaaaaac taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat
    10141 gctggaacag gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt
    10201 tacgccaacg ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta
    10261 ttatctcgca tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg
    10321 atgctatcgt ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt
    10381 tttaccgcta cgtcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc
    10441 gtccggtgct ggaagagttg cgtatatttt tcccggcgac gctggagctg gcttttggtg
    10501 ccctgctgct ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa
    10561 atcgctggct ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt
    10621 tctggcttgg gctgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg
    10681 gcggaaggct tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg
    10741 atgcgctgct tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac
    10801 cggcattaac gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga
    10861 tgctggaaca attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct
    10921 ggtatatcgt tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg
    10981 gtttggcgct gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc
    11041 ccggaatggg tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg
    11101 gctttgccgt cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct
    11161 atttgtggat tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac
    11221 gtccgccgta cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct
    11281 gaaaggcagt ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat
    11341 gattttttca ccgtggctgg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct
    11401 tttgccgcct tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag
    11461 ccgcgtactg gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc
    11521 gggtatgatt ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc
    11581 catcatcatg cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc
    11641 actggctgcc gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg
    11701 aattcccttt tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtatactta
    11761 tgttcaggcg gcgaaaacct ttggcgcgtc tcgttggcat ctgatcaact ggcatatttt
    11821 acgtaactcc ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt
    11881 aatggccgcc acgttgggat ttattggcct gggtgcacaa caaccgagtg ctgaatgggg
    11941 cgcgatggta gcgaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc
    12001 gggggcagcg attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga
    12061 tctgcttgac ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa
    12121 cagctgcatt tgagtttccc cggttttaac ggtgatgttc acgcgctcaa caatgtgtcc
    12181 ttgcagatta accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca
    12241 gtcaccgcaa tgctgattat gcgtctgcta cagacgggca gttattgcgt acatcggggg
    12301 cagatttcac tgctgggaga cgatgttctt aacgctcggg aaaaacagct tcgtcagtgg
    12361 cgcggcgcac gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacactt
    12421 cgaataggtc ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa
    12481 gccagagcta aagcgattgc cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt
    12541 atgtcgcgct atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg
    12601 gcattctcct gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc
    12661 acggtgcagt tgcaggtact gcgtttgctt aaacataaag cccgcgccag tggaactgcg
    12721 gttctgttca tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg
    12781 atgtatgccg gaagcgtaat tgaaagcggc gtgacggcag atgttatcca tcatccccgg
    12841 catccgtata ccattggttt gctccaatgc gcaccagaac atggagtacc acgccagcta
    12901 ttacccgcca ttccagggac ggtaccaaac ctcacccatt tgcctgaagg ctgcgctttt
    12961 cgcgatcgtt gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt
    13021 ggtgacaaca accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtgtctga
    13081 cacgttatta acgttacgcg acgtccatat caatttcccg gcccgtaaaa actggcttgg
    13141 taaaactacg gaacatgttc atgccattaa tggtattgat ttacagatcc gccgtggtga
    13201 aaccttaggg atcgtcggcg agtcaggctg cggcaaaagc accctcgcac agcttttaat
    13261 gggcatgctg caaccgagcc acgggcagta catccgttct ggctcacaac gcattatgca
    13321 gatggtgttt caggacccgc tctcttcgct caatccgcgc ttaccggtgt ggcgcatcat
    13381 cacagaaccg ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaga
    13441 ggagctggct gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt
    13501 ctccggcggg cagcggcaac gcatcgccat tgccagagca ctctcttcgc agcctgacgt
    13561 gattgtgctt gatgagccaa cctcggcgct ggatatctcc gtgcaggcgc agatcctcaa
    13621 tttactggta acgctacagg aaaatcacgg gctgacttat gtgctgattt cacacaatgt
    13681 ctcggtgata cgtcatatga gcgatcgggt ggcggtgatg tatctcgggc agattgtgga
    13741 actaggggaa gcgcaacagg tgttgactgc tccggcacat ccatataccc gattattgct
    13801 ggattccctc cccgccattg ataaaccgct ggaggaagaa tgggcattac gtaaaacgga
    13861 tctgccggga aaccgcacgt tgccgcaagg ctgttttttc tacgaacgtt gccctctggc
    13921 aacccacgga tgtgaagtcc ggcaatcatt aacaataagg gaggacggac gtgagatccg
    13981 gtgctggcgg gcgctgtaga gatgctaaca acgcatcagg cattcacgcg cctgacgcgt
    14041 catccggcaa tgctttacga tttcaactgg taatcaagcg taatttccgc tttcagtacc
    14101 tgagaaaccg ggcatcctgc ttttgctttc tggatgatgc cgtcaaaggt agaggcatca
    14161 atacccggca ccgcaacttc actcttcagt gcgattttcg taatcgcaaa accggcatcc
    14221 actttatcca gcgacacatc ggcggtggta tcaatcgatg ttggcgtaaa tcccgcttcc
    14281 cccagcatta atgaaagcgc cattgagaaa catgcggcat gcgctgcgcc aatcagttct
    14341 tcagggttag ttcctttttc gccttcaaaa cgcgtgttaa atccatacgg ctgttggttc
    14401 agcacaccac tctcggtgga tactgttccc ttcccgcgtt tgatatcgcc ttcccagtgt
    14461 gcctgacctt tcttatggat tgtcattgtt gctctcctgt gggcttaaaa aggaaattat
    14521 agactacgtg agaaacagca cgaggataag caggatattc cgaataaaaa tccggcagaa
    14581 atattacttc tgcacttaaa ttaaatttcc acaatagata accgggaata tataacccaa
    14641 gagatattgc attaatatta atcaactcac cacaataata atacctatca atatcatgag
    14701 gttaaaatat aaaaatcatc aaccaggact aatcttaaca acgaagcggc aaatatttgc
    14761 catgctgaat gtgctttata aatctgcgat ccgtagcaga caccataaat acacagacac
    14821 ggagaatcac tatgtttact tattatcagg cagaaaattc aacagcagaa cccgctctgg
    14881 ttaatgccat tgagcaaggc cttcgggcag agcacggtgt tgtcactgaa gatgacattc
    14941 tcatggagct gaccaaatgg gtggaagctt ctgataacga catcctcagt gatatctacc
    15001 agcaaacgat caattatgtg gtcagtggcc agcaccctac gctttaaggt gctatgcttg
    15061 atcggcaacc taatttaggg gtttagcacg tgtttcttcg ctacggcgat gttgtcctta
    15121 aaactagcaa caggattgag gagttaaaat gaaatcgaac cgtcaggcac gtcatattct
    15181 tggactggac cataaaattt ctaaccagcg caaaatagtt accgaaggtg acaaatccag
    15241 cgtggtaaat aatccaaccg gcagaaaacg ccccgctgaa aagtaattca taaccatcag
    15301 tcctcaatga cgattaaaca ccattgcctg cgcaatggtg tttttgtttt tatctgctct
    15361 atacttgggg ccgacgccct ggcggtaaag caaagacgat aaaagcgtgc cagggatgga
    15421 tattcaaaaa agagtgagtg acatggaacc aaaaacaaaa aaacagcgtt cgctttatat
    15481 cccttacgct ggccctgtac tgctggaatt tccgttgttg aataaaggca gtgccttcag
    15541 catggaagaa cgccgtaact tcaacctgct ggggttactg ccggaagtgg tcgaaaccat
    15601 cgaagaacaa gcggaacgag catggatcca gtatcaggga ttcaaaaccg aaatcgacaa
    15661 acacatctac ctgcgtaaca tccaggacac caacgaaacc ctcttctacc gtctggtaaa
    15721 caatcatctt gatgagatga tgcctgttat ttatacccca accgtcggcg cagcctgtga
    15781 gcgtttttct gagatctacc gccgttcacg cggcgtgttt atctcttacc agaaccggca
    15841 caatatggac gatattctgc aaaacgtgcc gaaccataat attaaagtga ttgtggtgac
    15901 tgacggtgaa cgtattctgg ggcttggtga ccagggcatc ggcgggatgg gcattccgat
    15961 cggtaaactg tcgctctata ccgcctgtgg cggcatcagc ccggcgtata cccttccggt
    16021 ggtgctggat gtcggaacga acaaccaaca gctgcttaac gatccgctgt atatgggctg
    16081 gcgtaatcca cgtatcactg acgatgaata ctatgaattc gttgatgaat ttatccaggc
    16141 tgtgaaacaa cgctggccgg acgtgctgtt gcagtttgaa gactttgctc aaaaaaatgc
    16201 gatgccgtta cttaaccgct atcgcaatga aatttgttct tttaacgatg acattcaggg
    16261 cactgcggca gtaacagtcg gcacactgat cgcagccagc cgcgcggcag gtggtcagtt
    16321 aagcgagaaa aaaatcgtct tccttggcgc aggttcagcg ggatgcggca ttgccgaaat
    16381 gatcatctcc cagacccagc gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt
    16441 tatggtcgat cgctttggcc tgctgacgga caagatgccg aacctgctac ctttccagac
    16501 caaactggtg cagaagcgcg aaaacctcag tgactgggat accgacagcg atgtgttgtc
    16561 actgctggat gtggtgcgca atgtaaaacc agatattctg attggcgtct caggacagac
    16621 cgggctgttt acggaagaga tcattcgtga gatgcataaa cactgtccgc gtccgatcgt
    16681 gatgccgctg tctaacccga cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg
    16741 gaccgaaggt aacgcgctgg tcgccactgg cagcccgttt aatccagtgg tatggaaaga
    16801 taaaatctac cctatcgccc agtgtaacaa cgcctttatt ttcccaggca tcgggctggg
    16861 tgttattgct tccggcgcgt cacgtatcac cgatgagatg ctgatgtcag caagtgaaac
    16921 gcttgctcag tattcgccgc tggtgctgaa cggcgaaggt ctggtactac cggaactgaa
    16981 agatattcag aaagtctccc gcgcaattgc gtttgcggtt ggcaaaatgg cgcagcagca
    17041 aggcgtggcg gtgaaaacgt ctgccgaagc tctgcaacaa gccattgacg ataatttctg
    17101 gcaagccgaa taccgcgact accgccgtac ctccatctaa gcctgcgccc ggtagtgaag
    17161 gctaccgggc tatttccctc tccctttttc agatctcatc catactgggt agtggcgaat
    17221 aaatctcatt tgcctcacct gctatgcaga acatcatccg aaaaggagga actatgaagg
    17281 ctgcagttgt tacgaaggat catcatgttg acgttacgga taaaacactg cgctcactga
    17341 aacatggcga agccctgctg aaaatggagt gttgtggtgt atgtcatacc gatcttcatg
    17401 ttaagaatgg cgattttggt gacaaaactg gggtaattct gggccatgaa ggcatcggtg
    17461 tggtggcaga agtgggtcca ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg
    17521 cgtggttcta cgaaggatgc ggtcattgcg aatactgtaa cagtggtaac gaaacgctct
    17581 gccgttcagt taaaaatgcc ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg
    17641 tggtcgccga ttacgcggta aaagtgccag atggtctgga ctcggcggcg gccagcagca
    17701 ttacctgtgc gggggtcacc acctacaaag ccgttaaact gtcaaaaatt cgtcccggtc
    17761 agtggattgc tatctacggt cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga
    17821 atgtctttaa cgccaaagtg atcgccattg atgtcaatga tgagcagtta aaactggcaa
    17881 ccgaaatggg tgcagattta gcgattaact cacgcaccga agacgccgcc aaaattgtgc
    17941 aggagaaaac cggtggtgca cacgctgcgg tggtgacagc ggtagctaaa gctgcgttta
    18001 actcggcagt tgatgctgtc cgtgcaggcg gtcgtgttgt ggctgtcggt ctgccgccgg
    18061 agtctatgag cctggatatc ccacgccttg tgctggatgg cattgaagtg gtcggttcgc
    18121 tggtcggcac gcgccaggat ctaaccgaag ccttccagtt tgccgccgaa ggcaaagtgg
    18181 tgccgaaagt cgccctgcgt ccgttagcgg acatcaacac catctttacc gagatggaag
    18241 aaggcaaaat ccgtggccgt atggtgattg atttccgccg ttaagaggcc tttgctgcga
    18301 gtgccagtcc tggtgtcgca gcatcgcact ctctccgaca ttaaatggct aaatggatta
    18361 ttgttccttc ctacgcaaca ataatgatag tgttcctgat gaaggaacac tactgagagt
    18421 aaagacactg atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
    18481 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac
    18541 ggcaagccga ttgctgaccg gaaaagcagc tttgacacca gaaatggcga ttaaactttc
    18601 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagtctggc
    18661 agaggcagaa aaaacggtgg atgtgtcgcg actacgccgt ctggttacgc aataaaaaaa
    18721 tggcgcttct taaagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc
    18781 actctctttt ttcgcttctg ccttctcgat ttcacgatac cagcgcggat ggtgtttctt
    18841 cgcccagcga cggctcacct tcccttcgat catcccttta atcgaccctt tcacccagaa
    18901 tgccatatac atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat
    18961 cagcaggctg tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat
    19021 aatcaccccg gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt
    19081 ttgcccggcg ttgtacttac cgacatccgc cactttatgc tcattgcctt tcaatacttc
    19141 gacaatgttc aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg
    19201 cacaaacata aacatcagtg cgacgaaaat cgcaatgccg aaaaacgggt gcaaaatgcg
    19261 tcccatctgc ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga
    19321 aatcccggac agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc
    19381 aataaatttg gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt
    19441 ccacttcctt attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa
    19501 taaagccagc cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga
    19561 tcttcggatc tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt
    19621 acataacgtg cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac
    19681 gcgctttcag tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga
    19741 agtggatagc cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc
    19801 gatcgacgca gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat
    19861 taaacggaca cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga
    19921 caatcccgtt agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagccgg
    19981 gatcttcaca gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt
    20041 cggtttcgct aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt
    20101 aaaccccgac gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct
    20161 gacaggcttt acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt
    20221 aatcacgcac ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct
    20281 gcgtttccat agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt
    20341 tatattccgg cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat
    20401 aacctttacg cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct
    20461 gctgaccatt tacattcagt ggtttcagac gacgcgttac cacagccacc gcgcggataa
    20521 agccacgctt gcttgagaca gtgacacgat cgccattatt aatgcctttc gccgccgcca
    20581 gcgtttcgct gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct
    20641 tggtccaggt gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct
    20701 cttttttacc catccgcagt gcgtcttgtt catacagacg aacaaccggg ttagacacca
    20761 cgttcggatg cagcgggtta gtgcccagcg gcgtttcaat cggctcgtag tgttccggga
    20821 acggaccttc cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga
    20881 taaacggccc ggttggcgta cccggtgcgg cattgccgaa gtcaggaata tcgttacccg
    20941 tccacttgct gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga
    21001 tatccgccga agcacggttg tacagcacgc gacggttgag cggccacgcc caggcccatc
    21061 ccagcgtatt ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct
    21121 gctctgtcca gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca
    21181 gatgcgcaaa gctactcagc aactgacctt tcttcgcaat cagcacgcca ttagcatcat
    21241 agagatcttc cagcgcatag ccgttgttct ctttagctac ttcgtcagat tgcggttcgt
    21301 gcggctgctt gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccttcgg
    21361 cctggtacag ctcgcgcagg tgatggtaga tacccgccag aatttcaccg tcgttacgcg
    21421 cttcgcccgg cgcatcctga cctttccagt gccactgcag ccagcgaccg gagttagcaa
    21481 tagaaccatc ttcttcagca aagcaggtcg aaggcagacg gaatacttca gtctgaatag
    21541 acgccggatc gacatcgttc gactcgccgt ggttctgcca gaaggttgag gtttcagtca
    21601 ccagcggatc gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt
    21661 tgtccgggaa ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttgcctt
    21721 catccatcat gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt
    21781 catagcccca gttgttctct ttctgcgcgg catcgccata gaaagatttc atcaggctaa
    21841 cgaagaactt cggatagttg ctccagtagt tcacctgacc agccagcgtc gctttcggcg
    21901 tgttcgcttc cagatacgac tgcaaatcaa cctgtttttc tgacggcagc gtcagataac
    21961 ctggcaggct ggtagagagc agacctaagt cagtcagacc ctgaatgttg gagtgaccac
    22021 gcaatgcgtt cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg
    22081 ccatagtacg gatgttctgc gcacccacgg tgtgctgcgt ccagcccagc gcgtacagga
    22141 aggtggttgt gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt
    22201 cggcttttgg cgtaccacag atgttttcaa caacgtccgg cgtgtagcgg gaaacgtgcg
    22261 ctttcagcag gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt ttcgcatagc
    22321 cgttttcatc gaactgatag ttccaggacg atttatcgta ttgccgtttt tcagcgtcgt
    22381 agccgctgaa cagaccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg
    22441 tgtaatgttt aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc
    22501 cagacaggaa cgtaatgtcc gtaccggaac gaataggtgc gtaaatatcc gccacagaag
    22561 cggtacgcgt aaaacgggga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt
    22621 ccatcgccca gcggaaaccg acgggatgcg cttcagcagc gttaccgccc attaccatta
    22681 cgacgttagc gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg
    22741 gagcaagact tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca
    22801 tcccgaggga gcgggcaaat ttctgtgtca gcatcccggt ttcgttgctg gcaccggagg
    22861 cacacagcat accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa
    22921 taaagttagc gtcacggtca gctttcatca gctttgcaat acgggagaat gcttcttccc
    22981 agctaatgcg ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt
    23041 tttcgctgtt gacgtaatcc agcaaaccgg ccccttttgg gcacagcgca ccacggctta
    23101 ccggatgatc cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt ttcgcgccat
    23161 cacccaggct atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga
    23221 tctctttagc gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa
    23281 agcccaatgc cgctaccgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc
    23341 tgcgactgac gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa
    23401 cagtaaaatt ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata
    23461 atccgcctgc gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca
    23521 ggccagttaa aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac
    23581 agcgagcatg acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag
    23641 cacgatggta ggattgattc gcggtgtcag cgaggggctc ggcccggtcg gcggcgcagc
    23701 tgctatctat tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg
    23761 gcagattccg aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg
    23821 tctggcgctt tccttagggt atgcggcgac ccgtcatcag gcgattgaag tgggtatggt
    23881 gaactatcta tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac
    23941 caactggctg attgtaccag gattattatt agccctggtc ggcgtctgct gggtgttagg
    24001 cggtgataat gggttacatt atgatgaaat catcaataat attaccacca gcccattgag
    24061 ttatttcctg gcgtttattg gtgcgtttat ctgggcagcc tattgcacag taacgaataa
    24121 atacgcacgc ggatttaacg gaattaccgt ttttgtcctg ctaacgggag caagtctgtg
    24181 ggtttactat tttctcacgc cacaaccaga aatggtattt agcacgcccg tcatgattaa
    24241 actcatctct gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatact
    24301 gcatggcaat gtcaccataa tggcggtagg ctcgtatttt acgcccgtac tttcctcagc
    24361 gcttgcagcc gtgctgctca gcgccccgct gtcgttctca ttctggcaag gcgcgctaat
    24421 ggtctgcggc ggttccctgc tctgctggct ggcgacacgt cgtggttaaa ataattgccg
    24481 ggtc
//
LOCUS       DAZFVO020000066        24340 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFVO020000066 DAZFVO020000000
VERSION     DAZFVO020000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.066"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            78..377
                     /locus_tag="ACHKNB_RS12310"
                     /old_locus_tag="pgaptmp_002462"
     CDS             78..377
                     /locus_tag="ACHKNB_RS12310"
                     /old_locus_tag="pgaptmp_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4195957.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(424..1311)
                     /gene="rarD"
                     /locus_tag="ACHKNB_RS12315"
                     /old_locus_tag="pgaptmp_002463"
     CDS             complement(424..1311)
                     /gene="rarD"
                     /locus_tag="ACHKNB_RS12315"
                     /old_locus_tag="pgaptmp_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HGU4195958.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAISAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1363..1830)
                     /gene="yigI"
                     /locus_tag="ACHKNB_RS12320"
                     /old_locus_tag="pgaptmp_002464"
     CDS             complement(1363..1830)
                     /gene="yigI"
                     /locus_tag="ACHKNB_RS12320"
                     /old_locus_tag="pgaptmp_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HGU4195959.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1995..2864
                     /gene="pldA"
                     /locus_tag="ACHKNB_RS12325"
                     /old_locus_tag="pgaptmp_002465"
     CDS             1995..2864
                     /gene="pldA"
                     /locus_tag="ACHKNB_RS12325"
                     /old_locus_tag="pgaptmp_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HGU4195960.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2997..4826
                     /gene="recQ"
                     /locus_tag="ACHKNB_RS12330"
                     /old_locus_tag="pgaptmp_002466"
     CDS             2997..4826
                     /gene="recQ"
                     /locus_tag="ACHKNB_RS12330"
                     /old_locus_tag="pgaptmp_002466"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HGU4195961.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4890..5510
                     /gene="rhtC"
                     /locus_tag="ACHKNB_RS12335"
                     /old_locus_tag="pgaptmp_002467"
     CDS             4890..5510
                     /gene="rhtC"
                     /locus_tag="ACHKNB_RS12335"
                     /old_locus_tag="pgaptmp_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HGU4195962.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5572..6192)
                     /gene="rhtB"
                     /locus_tag="ACHKNB_RS12340"
                     /old_locus_tag="pgaptmp_002468"
     CDS             complement(5572..6192)
                     /gene="rhtB"
                     /locus_tag="ACHKNB_RS12340"
                     /old_locus_tag="pgaptmp_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HGU4195963.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6303..7325
                     /gene="pldB"
                     /locus_tag="ACHKNB_RS12345"
                     /old_locus_tag="pgaptmp_002469"
     CDS             6303..7325
                     /gene="pldB"
                     /locus_tag="ACHKNB_RS12345"
                     /old_locus_tag="pgaptmp_002469"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HGU4195964.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7333..8133
                     /gene="yigL"
                     /locus_tag="ACHKNB_RS12350"
                     /old_locus_tag="pgaptmp_002470"
     CDS             7333..8133
                     /gene="yigL"
                     /locus_tag="ACHKNB_RS12350"
                     /old_locus_tag="pgaptmp_002470"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HGU4195965.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8209..9108
                     /gene="bioP"
                     /locus_tag="ACHKNB_RS12355"
                     /old_locus_tag="pgaptmp_002471"
     CDS             8209..9108
                     /gene="bioP"
                     /locus_tag="ACHKNB_RS12355"
                     /old_locus_tag="pgaptmp_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HGU4195966.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQLHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8996..9949)
                     /gene="metR"
                     /locus_tag="ACHKNB_RS12360"
                     /old_locus_tag="pgaptmp_002472"
     CDS             complement(8996..9949)
                     /gene="metR"
                     /locus_tag="ACHKNB_RS12360"
                     /old_locus_tag="pgaptmp_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HGU4195967.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10067..12328
                     /gene="metE"
                     /locus_tag="ACHKNB_RS12365"
                     /old_locus_tag="pgaptmp_002473"
     CDS             10067..12328
                     /gene="metE"
                     /locus_tag="ACHKNB_RS12365"
                     /old_locus_tag="pgaptmp_002473"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGU4195968.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEVQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12367..13182)
                     /gene="ysgA"
                     /locus_tag="ACHKNB_RS12370"
                     /old_locus_tag="pgaptmp_002474"
     CDS             complement(12367..13182)
                     /gene="ysgA"
                     /locus_tag="ACHKNB_RS12370"
                     /old_locus_tag="pgaptmp_002474"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HGU4195969.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13444..14205
                     /gene="udp"
                     /locus_tag="ACHKNB_RS12375"
                     /old_locus_tag="pgaptmp_002475"
     CDS             13444..14205
                     /gene="udp"
                     /locus_tag="ACHKNB_RS12375"
                     /old_locus_tag="pgaptmp_002475"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HGU4195970.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14346..15773
                     /gene="rmuC"
                     /locus_tag="ACHKNB_RS12380"
                     /old_locus_tag="pgaptmp_002476"
     CDS             14346..15773
                     /gene="rmuC"
                     /locus_tag="ACHKNB_RS12380"
                     /old_locus_tag="pgaptmp_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGU4195971.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15868..16623
                     /gene="ubiE"
                     /locus_tag="ACHKNB_RS12385"
                     /old_locus_tag="pgaptmp_002477"
     CDS             15868..16623
                     /gene="ubiE"
                     /locus_tag="ACHKNB_RS12385"
                     /old_locus_tag="pgaptmp_002477"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HGU4195972.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16637..17242
                     /gene="ubiJ"
                     /locus_tag="ACHKNB_RS12390"
                     /old_locus_tag="pgaptmp_002478"
     CDS             16637..17242
                     /gene="ubiJ"
                     /locus_tag="ACHKNB_RS12390"
                     /old_locus_tag="pgaptmp_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HGU4195973.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17239..18879
                     /gene="ubiB"
                     /locus_tag="ACHKNB_RS12395"
                     /old_locus_tag="pgaptmp_002479"
     CDS             17239..18879
                     /gene="ubiB"
                     /locus_tag="ACHKNB_RS12395"
                     /old_locus_tag="pgaptmp_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HGU4195974.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18958..19227
                     /gene="tatA"
                     /locus_tag="ACHKNB_RS12400"
                     /old_locus_tag="pgaptmp_002480"
     CDS             18958..19227
                     /gene="tatA"
                     /locus_tag="ACHKNB_RS12400"
                     /old_locus_tag="pgaptmp_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HGU4195975.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19231..19746
                     /gene="tatB"
                     /locus_tag="ACHKNB_RS12405"
                     /old_locus_tag="pgaptmp_002481"
     CDS             19231..19746
                     /gene="tatB"
                     /locus_tag="ACHKNB_RS12405"
                     /old_locus_tag="pgaptmp_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HGU4195976.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19749..20525
                     /gene="tatC"
                     /locus_tag="ACHKNB_RS12410"
                     /old_locus_tag="pgaptmp_002482"
     CDS             19749..20525
                     /gene="tatC"
                     /locus_tag="ACHKNB_RS12410"
                     /old_locus_tag="pgaptmp_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HGU4195977.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20567..21349
                     /gene="tatD"
                     /locus_tag="ACHKNB_RS12415"
                     /old_locus_tag="pgaptmp_002483"
     CDS             20567..21349
                     /gene="tatD"
                     /locus_tag="ACHKNB_RS12415"
                     /old_locus_tag="pgaptmp_002483"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HGU4195978.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21346..21834)
                     /gene="rfaH"
                     /locus_tag="ACHKNB_RS12420"
                     /old_locus_tag="pgaptmp_002484"
     CDS             complement(21346..21834)
                     /gene="rfaH"
                     /locus_tag="ACHKNB_RS12420"
                     /old_locus_tag="pgaptmp_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HGU4195979.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22001..23494
                     /gene="ubiD"
                     /locus_tag="ACHKNB_RS12425"
                     /old_locus_tag="pgaptmp_002485"
     CDS             22001..23494
                     /gene="ubiD"
                     /locus_tag="ACHKNB_RS12425"
                     /old_locus_tag="pgaptmp_002485"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HGU4195980.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23540..24241
                     /gene="fre"
                     /locus_tag="ACHKNB_RS12430"
                     /old_locus_tag="pgaptmp_002486"
     CDS             23540..24241
                     /gene="fre"
                     /locus_tag="ACHKNB_RS12430"
                     /old_locus_tag="pgaptmp_002486"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HGU4195981.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
BASE COUNT         5733 a         6034 c         6667 g         5906 t
ORIGIN      
        1 atggggtata ttttaattaa atataattga aatgtatttt tattctataa acaatatctt
       61 gggtgaatgg attctatatg ggaaatggta acttttggca gttgttcttg ggtaaagaac
      121 cttacagcaa aaaatctgat tttgttaagg catggttatt gtggtgggct attaccatcc
      181 ttgttctatt tattttaagc ctgactacga cattaaattt aagaagtata ggtatagggt
      241 taagttttgg gttttgtcaa atccttggta gatatacgat gtctctagga tggagtaagt
      301 actggggagt tttgggggta tttcctgtaa caaatattgt tctttttctt atcttaattt
      361 ttttcaaaaa aagataaata agtcagtggg taggctcttt aaacggccta cccatgttaa
      421 ttcttaactt tttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac
      481 ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac
      541 agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcctaacg ttgagagacg
      601 caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg gtagtgacaa taccggcggc
      661 gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg agctgtcggc
      721 aatagcaaac aggtaaattg ccgccacggg cagcagccac atggtttcga ttaacatgcc
      781 ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg tagaaggcaa aactaaatgc
      841 cagtcccagc gcgataatag gtagcgaacc aaaagtccac agctggacta acacgccaca
      901 tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat
      961 ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg
     1021 gtgattgttc accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagat
     1081 tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg
     1141 gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat
     1201 ttcatcggca ggcacgtagt aaatcaactt gaagtacgct ggcgctatac cccaaataaa
     1261 ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac
     1321 tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt
     1381 ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc gggcgacggc
     1441 gactttattg cctgcacgca acaggctact ggtagcagta aaacgctcgc ccctgcctgg
     1501 gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct ggcgtagttc
     1561 atcttcactg atggtttcgt ggcgggttaa ggtacttccc acgcacacca gaccggcggc
     1621 gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc agttgcccac
     1681 catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac gctccagttc
     1741 catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca ctaatttcag
     1801 ggcttgttca gcggtcagta cggcagacat atcatcctta cacttcattg gttaatgaaa
     1861 tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa ctaacggcta
     1921 tggagttaag tatgtaaaat agcccgcaga aaaatattca ccttatcaat aattcgttac
     1981 ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt atgttgccta
     2041 tggcagtata tgcgcaagag gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg
     2101 gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc tatccttatg
     2161 acaccaacta cctcatttac acccaaacca gcgatctgaa taaagaagcg attgccagtt
     2221 acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc
     2281 cgctgtggcg tgggatttta ggcccgaact cggtgttggg tgcgtcttat acgcaaaaat
     2341 cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac
     2401 cgcaattgtt cctcggtttt gccaccgatt accgttttgc aggttggacg ctgcgcgatg
     2461 tggagatggg gtataaccac gactctaacg ggcgttccga cccgacctcc cgcagctgga
     2521 accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa gtgaagccgt
     2581 ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg ggttactacc
     2641 agcttaaaat cggctatcac ctcggtgatg cggtgctcag tgcgaaagga cagtacaact
     2701 ggaacaccgg ctacggcggc gcggagttag gcttaagtta cccgatcacc aaacatgtgc
     2761 gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat aacttcaacc
     2821 agacccgtgt cggtgtgggg gttatgctaa acgatttgtt ttgatgaacg gttgagtggt
     2881 tggcaaatct ggaatccagc atccaggatt accctctcag agactaaaag cattgcagtt
     2941 tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg
     3001 cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa gaaacctttg
     3061 gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt tccggtcgcg
     3121 attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa attcctgcct
     3181 tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg aaagatcagg
     3241 tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg
     3301 aacagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgtctg ctttatatcg
     3361 ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg aatccggtgt
     3421 tattagccgt tgatgaagcg cactgtatct cccaatgggg ccacgatttc cgcccggaat
     3481 atgccgcgct cggtcagttg cgccagcggt tcccgacgct gccgtttatg gcgctgaccg
     3541 ccacagccga cgacaccacg cgccaggata tcgtgcgcct gctggggctg aacgatccgc
     3601 tgattcaaat cagcagtttt gaccgtccga atattcgcta catgctgatg gagaagttca
     3661 aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtcc ggcattatct
     3721 actgcaacag ccgggcgaaa gtagaagaca ccgctgcgcg cctgcaaagc aagggtatta
     3781 gcgcggcggc ctatcatgcc gggctggaaa ataatgtccg cgccgacgtg caggagaaat
     3841 tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg ggcatcaata
     3901 aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa tcctattatc
     3961 aggaaaccgg tcgcgccggg cgtgatggcc tgcccgcgga agcgatgctg ttttacgatc
     4021 cggccgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg cagttgcagg
     4081 atatcgaacg tcataaactc aatgcgatgg gcgcttttgc cgaagcgcaa acttgccgtc
     4141 gtctggtact gctgaactat tttggcgaag ggcgtcagga gccgtgcggg aactgcgata
     4201 tctgcctcga tccgccgaaa cagtacgacg gttcaaccga tgctcagatt gccctttcca
     4261 ccattggtcg tgtgaatcag cgttttggta tgggctatgt ggtggaagtg attcgcggcg
     4321 ctaataacca gcgtatccgc gactatggtc atgacaaact aaaagtctac ggtatgggcc
     4381 gtgataaaag tcatgaacat tgggtgagcg tgatccgcca gctgattcac ctcggtctgg
     4441 tgacgcaaaa tattgcccag cattctgccc tacaactgac agaggctgcg cgtccggtgc
     4501 tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag
     4561 cgatgcagaa atcgttcggc ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac
     4621 tgcgtaaatc gatagccgat gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa
     4681 ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc agcgttaacg
     4741 gcgttgggat gcgcaagctg gaacgctttg gcaaaccgtt tatggcgctg attcgtgcgc
     4801 atgttgatgg cgatgacgaa gagtagtcag cagcataaaa aagtgccagt atgaagactc
     4861 cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg
     4921 tgcacattgt ggcgcttatg agccccggtc ccgatttctt ttttgtctct cagaccgctg
     4981 tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg
     5041 tttgggctgg gattgcgctg cttggcctgc atttgattat cgaaaaaatg gcctggctgc
     5101 atacgctgat tatggtgggc ggtggcctgt atctctgctg gatgggttac cagatgctac
     5161 gcggtgcact gaaaaaagag gcggtttctg cacctgcgcc acaggtcgag ctggcgaaaa
     5221 gtgggcgcag tttcctgaaa ggtttactga ccaatctcgc taatccgaaa gcgattatct
     5281 actttggctc ggtgttctca ttgtttgtcg gtgataacgt tggcactacc gcgcgctggg
     5341 gcatttttgc gctgatcatt gtcgaaacgc tggcgtggtt taccgtcgtt gccagcctgt
     5401 ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc gaagtggatt gatggttttg
     5461 ccggggcgtt atttgccgga tttggcattc atttgattat ttcgcggtga tgccagacgc
     5521 gtcttcagag taagtcggat aaggcgttta cgccgcatcc gacattattt ttcacgcatg
     5581 cctcgccgat gctaacagcg ctcccaccag cataaacaac gagccgaaaa tcttattcag
     5641 cgccttcatc tgctttggtc ctttaatcca tagagcaatc cgttgagcaa gggtggcgta
     5701 accgatcatc acaataatat cgaccacaat agtggtgacg ccgagcacga tatactgcat
     5761 cagttgcggc tgttgcggca tgatgaattg cggaaatagc gccgccagaa acacaatact
     5821 tttgggattg gtgagattca caaaaactgc gcgctggaac aaatgtcgac gcgattgagt
     5881 agaggccagc gatttaaggt caattgcacc agcggcgcgc cactgctgga ttcccagcca
     5941 aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac gcaatcactg agcgggaaaa
     6001 tagcgtcccc aaccccacgc caaccaacac aatatgaatc gccagtccgg tctgaagccc
     6061 agcaatagac gccaccgcgc cgcgataacc gtggttgagc gaggtggtca tagtgttgat
     6121 tgcaccagag cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg caaaccacca
     6181 ttctaaggtc atgatgaact cccggtgtgt ctatttttgt gccacaatac gctactgtcg
     6241 cagcgttgtg tcaggcacgc taaaaaaaac gattttacgt ggtttaagag gcagattacc
     6301 cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa cgcgtttgct gcttttacca
     6361 tgggaccgct gactgatttc tggcgtcagc gtgatgaagc agagtttact ggtgtggatg
     6421 acattccggt gcgctttgtc cgttttcgcg cacagcacca tgaccgggtg gtagtcatct
     6481 gcccggggcg tattgagagc tacgtaaaat atgcggaact ggcctatgac ctgttccatt
     6541 tggggtttga tgtcttaatc atcgaccatc gcgggcaggg acgttccggt cgcctgttag
     6601 ccgatccgca tctcgggcat gttaatcgct ttaatgatta tgttgatgat ctggcggcat
     6661 tctggcagca ggaggttcag cccggtccgt ggcgtaaacg ctatatactg gcacattcga
     6721 tgggcggtgc gatctccaca ttatttctgc aacgccatcc aggtgtatgt gacgccattg
     6781 cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc gtcatttatg gcacggcaga
     6841 tcctcaactg ggccgaagcg catccacgtt tccgtgatgg ctatgcaata ggcaccgggc
     6901 gctggcgcgc gttgccgttt gctatcaacg tactgaccca cagcagacag cgatatcgac
     6961 gtaacttacg cttctatgct gatgacccaa cgattcgcgt cggtgggccg acctaccatt
     7021 gggtacgcga aagtattctg gctggcgaac aggtgttagc cggtgcgggt gatgacgcca
     7081 cgccaacgct tctcttacaa gctgaagagg aacgcgtggt ggataaccgc atgcatgacc
     7141 gtttttgtga actccgcacc gccgcgggcc atcctgtcga aggaggacgg ccgttggtaa
     7201 ttaaaggtgc ttaccatgag atcctttttg aaaaggacgc aatgcgctca gtcgcgctcc
     7261 acgccatcgt tgattttttc aacaggcata actcacccag cggaaaccgc tctacagagg
     7321 tttaaatttc ttatgtacca ggttgttgcg tctgatttag atggcacgtt actttctccc
     7381 gaccatacgt tatcccctta cgccaaagaa actctgaagc tgctcaccgc gcgcggcatc
     7441 aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg ggcaaattcg cgataatctg
     7501 gagattaagt cttacatgat tacctccaat ggtgcgcgcg ttcacgatct ggatggtaat
     7561 ctgatttttg ctcataacct ggatcgcgac attgccagcg atctgtttgg cgtagtcaac
     7621 gacaatccgg acatcattac taacgtttat cgcgacgacg aatggtttat gaatcgccat
     7681 cgcccggaag agatgcgctt ttttaaagaa gcggtgttca aatatgcgct gtatgagcct
     7741 ggattactgg agccggaagg cgtcagcaaa gtgtttttca cctgcgattc ccatgaacaa
     7801 ctgctgccgc tggagcaggc gattaacgct cgctggggcg atcgcgtcaa cgtcagtttc
     7861 tctaccttaa cctgtctgga agtgatggcg ggcggcgtgt cgaaaggcca tgcgctggaa
     7921 gcagtggcga agaaactggg ctacagcctg aaggattgta ttgcgtttgg tgacgggatg
     7981 aacgacgccg aaatgctgtc gatggcgggg aaaggctgca ttatgggcag tgcgcaccag
     8041 cgtctgaaag accttcatcc cgagctggaa gtgattggta ctaatgccga cgacgcggtg
     8101 ccgcattatc tgcgtaaact ctatttatcg taatcgttct ttatttggtc agttgtcaac
     8161 ctgatacttc gctacaatgg atactcgtta atcaaagagt tttccattgt ggcgctactt
     8221 atcatcacca cgattctgtg ggccttctcc tttagctttt atggcgagta ccttgcgggg
     8281 cacgtcgata gctattttgc ggtgctggtg cgcgttggcc tggcggcact cgtttttctg
     8341 ccgtttctgc gtacccgtgg caatagcctg aaaacggtcg gcctgtatat gttggtgggc
     8401 gcgatgcagc tcggcgtgat gtatatgctg agtttccgcg cgtatctcta cctgacggtc
     8461 tccgaactgc tgctgttcac cgtactgacg ccgctctaca tcacgctgat ttatgacatc
     8521 atgagtaagc gccgtctgcg ctggggctat gcttttagcg ccttgctggc ggtgattggt
     8581 gccgggatta ttcgctatga tcaggtcacc gaccatttct ggactggctt gttgctggtg
     8641 caactctcca atatcacttt tgccatcggc atggtgggtt acaaacgcct gatggaaact
     8701 cgcccgatgc cgcagcataa cgcctttgcg tggttctatc ttggcgcgtt cctggtggcg
     8761 gtaattgcat ggttcttgct gggaaatgcg cagaaaatgc cgcaaaccac gttgcaatgg
     8821 ggcattctgg tgtttcttgg cgtggtggct tccgggattg gctactttat gtggaactac
     8881 ggcgcgacgc aggtggacgc cggaacgctg ggcattatga ataatatgca cgttccggca
     8941 gggctgctgg ttaacctggc tatctggcac caacagctgc actggccaac gtttattaca
     9001 ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta agtgggtcgc tccgcgctct
     9061 tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga gcgaataaac gcttccgtaa
     9121 ctggctgacg ctgctcgcca tcgcgcacgg cggcgtacag tcggctccac aagccttcgc
     9181 ccagggtttt ggtcaccacc agaccctggc gctcaaaact ctctactacc caatgcggta
     9241 gcgcggcaat acccatccgc gcggcaacca tctgaatcaa caataaggtg ttatcgacgc
     9301 ttttcagtga cgggctgacg cctgccggct gaagaaaatg ccgccagaca tccagtcgac
     9361 tacgctgcac cggataaatt aatagcgtct cgctggcgag atcttccggt gtaatacgcg
     9421 tttttgccgc cagtggatgg tcaggtgcta acaccaggcg tacttcataa tcgaacatcg
     9481 gcgaataatg caggccactg cgcggcagga tatcggaggt catcaccaga tccagctcgc
     9541 cctgttgcaa cgcgggctgc gggtcaaacg tcacgcccga tttaaaatcc atctctacct
     9601 gcggccagtt cttatggaaa ttttctaacg cgggtgtcag ccactgaata cagctatggc
     9661 actcaatggc aatgcgcaga cgcgtctgct gcggttcatt gcaggcttgt agtgcctggc
     9721 tgatttgcgg cagcacctgg tttgccagtt gcaacaggat ttctccctgc ggtgtaaagc
     9781 gtagcggctg gctcttacgc acaaatagcc ggaaaccaag gcgttgttcc agatcgctaa
     9841 actggtgaga cagggcggat tgcgtctgat gcaacgtcgc cgcagcggct gcgagcgagc
     9901 cgcagttccg caacgcttgt agcgttttca ggtgttttac ttcgatcatg aaagtccttc
     9961 acttcggcat gaataatttg cgcttgagga atatacagta accgccaatt atggatgtgt
    10021 aaacatctgg acggctaaat actaaaaatt ccataagggg catataatga caattcttaa
    10081 tcacaccctc ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag
    10141 ttattgggcg gggaactcca cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc
    10201 tcgtcactgg gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg gcgattttgc
    10261 ctggtacgat catgtactga ccaccagttt gctgctgggt aatgttccgc cacgtcatca
    10321 gaacaaagac ggttcggtag atatcgacac cctgttccgt attggtcgtg gacgtgcgcc
    10381 gactggcgaa cctgcggcgg cagcggaaat gaccaaatgg tttaacacca actatcacta
    10441 catggtgccg gagttcgtta aaggccaaca gttcaaactg acctggacgc agctgctgga
    10501 ggaagtggac gaggcgctgg cgctgggcca caacgttaag cctgtgctgc tggggccggt
    10561 tacttacctg tggctgggga aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa
    10621 cgacattctg ccggtttatc agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg
    10681 ggtacagatt gatgaacccg cgctggtact ggaactacca caggcgtggc tggacgcata
    10741 caaacccgct tacgacgcgc tccagggaca ggtgaaactg ctgctgacca cctattttga
    10801 aggcgtaacg ccaaatctcg acacgattac tgcgctgcct gttcagggtc tgcatgttga
    10861 cctcgtacat ggtaaagatg acgttgctga actgcacaag cgcctgcctt ctgactggtt
    10921 gctgtctgcg ggtctgatca atggtcgtaa cgtctggcgc gccgatctta ccgagaaata
    10981 tgcgcaaatt aaggacattg tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt
    11041 gctgcacagc cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg
    11101 gtttgccttc gccctacaaa aatgccatga actggcactg ctgcgcgatg cgctgaacag
    11161 tggtgacacg gcagctctgg cagagtggag cgccccaatt caggcgcgtc gtcactctac
    11221 tcgtgtacat aatccggcgg tagaaaagcg tctggcggcg atcaccgccc aggacagcca
    11281 gcgtgcgaat gtctatgaag tgcgtgctga agtccagcgt gcgcgtttta aactgccagc
    11341 gtggccgacc accacgattg gttccttccc gcaaaccacg gaaattcgta ccctgcgtct
    11401 ggatttcaaa aagggcaatc tcgacgccaa caactaccgc acgggcattg cggaacatat
    11461 caagcaggcc attgttgagc aggaacgttt gggactggat gtgctggtac atggcgaggc
    11521 cgagcgtaat gacatggtgg aatactttgg cgagcacctc gacggatttg tctttacgca
    11581 aaacggttgg gtacagagct acggttcccg ctgcgtgaag ccaccgattg ttattggtga
    11641 cgtgagccga ccggcaccga ttaccgtgga gtgggcgaaa tatgcgcagt cgctgaccga
    11701 caaaccggtg aaagggatgc tgactggtcc ggtgacgatt ctctgctggt cgttcccgcg
    11761 tgaagatgtg agccgtgaaa ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt
    11821 ggcggacctg gaagccgctg gaattggcat catccagatt gatgaaccgg cgctgcgcga
    11881 aggtttaccg ctgcgtcgta gcgactggga cgcctatctc cagtggggcg tagaggcctt
    11941 ccgtatcaac gccgccgtgg cgaaagatga cacacaaatc cacactcaca tgtgttattg
    12001 cgagttcaac gacatcatgg attccattgc ggcgctggat gcggatgtga ttaccatcga
    12061 aacttcgcgt tctgacatgg agttgctgga gtcgttcgaa gagtttgatt atcccaatga
    12121 aatcggtcca ggcgtctatg atattcactc gccaaacgta ccgagcgtgg aatggattga
    12181 agccctgctg aagaaagcgg caaaacgcat tccggcagag cgcctgtggg ttaacccgga
    12241 ctgtggcctg aaaacgcgcg gctggccaga aacccgcgcg gcactggcga acatggtgca
    12301 ggcggcgcag aacttgcgtc gggggtaaat ccaaaccggg tggtaatacc acccggtctt
    12361 ttctcattac agcgacttct tcccaccata ctgcttaaac cattccagca tacgctgcca
    12421 gccatctttc gcagattcgg catgatagct cgggcgataa tcagcgttga atgcatgccc
    12481 ggcatccggg tacacgataa tctctgcttt cgcattagca gcccgcagcg cctggcgcat
    12541 ggtttcaacg ctctcctgcg gaatgctgtt atcctgacca ccatataagc cgagaaccgg
    12601 cgcgttaaga tcggttgcga tatcaacagg ttgtttcggt gaattcagcg acttgtcgcc
    12661 cgtcagtttg ccgtaccacg ccactgcggc ttttagctgt ggattatgcg cggcatacag
    12721 ccaggtgata cgtccacccc agcagaatcc ggtgatcatt aaacgatgaa catcgccgcc
    12781 gttgcgggaa gcccaactgg cgacatgatc gagatcggcc agcacctgcg agtcaggcac
    12841 ttttgctacc agaccgctaa gcaacgtggg gatatcggca aaatcattcg gatcgccttc
    12901 gcggaagtaa agttcaggtg cgatagccag atacccctcc agcgccagac ggcgacaaat
    12961 gtcgcggata tgttcatgca cgccaaaaat ttcctgcact acaatgacca ctggcagtgg
    13021 gccatcgctt tgctttggtc tggcatggta agcaggcatg ttatcccctt gtgaagggat
    13081 agaggtgaag cccgccacaa tcgcgtcgtc cggggtctga acgatggtcg aagcgagagg
    13141 cgatgcagca ggtgcaaatc cagattgttg tgttgttgcc atggtattct ccgtaccctt
    13201 ataaaaatgt tgcgcaatgt taactatagt cagcatgcaa caaatcacat tgcctgaatc
    13261 ggctcatctt ttatgcagtc ctgcagaatg aagagtgatt tatgtgattt acatcacttt
    13321 tgatgggtaa atttatgcaa cgcgtttgcg tcatggtgat gagtatcacg aaaaaatgtt
    13381 aaacccttcg gtaaagtgtc tttttgcttc ttctgactaa accgattcac agaggagttg
    13441 tatatgtcca agtctgatgt ttttcatctc ggcctcacta aaaacgattt acaaggggct
    13501 acgcttgcca tcgtccctgg cgacccggat cgtgtggaaa agatcgccgc gctgatggat
    13561 aagccggtta agctggcatc tcaccgcgaa ttcactacct ggcgtgcaga gctggatggt
    13621 aaacctgtta tcgtctgttc taccggtatc ggcggcccgt ctacctctat tgctgttgaa
    13681 gagctggcac agctgggcat tcgcaccttc ctgcgtatcg gtacaacggg cgctattcag
    13741 ccgcatatta atgtgggtga tgtcctggtt accacggcgt ctgtccgtct ggatggcgcg
    13801 agcctgcact tcgcaccgct ggaattcccg gctgtcgctg atttcgaatg tacgactgcg
    13861 ctggttgaag ctgcgaaatc cattggcgcg acaactcacg ttggcgtgac agcttcttct
    13921 gataccttct acccaggtca ggaacgttac gatacttact ctggtcgcgt agttcgtcac
    13981 tttaaaggtt ctatggaaga gtggcaggcg atgggcgtaa tgaactatga aatggaatct
    14041 gcaaccctgc tgaccatgtg tgcaagtcag ggcctgcgtg ccggtatggt agcgggtgtt
    14101 atcgttaacc gcacccagca agagatcccg aatgctgaga cgatgaaaca aaccgaaagc
    14161 catgcggtga aaatcgtggt ggaagcggcg cgtcgtctgc tgtaattctc ttctcctgtc
    14221 tgaaggccga cgcgttcggc cttttgtatt tttgcgtagc gcctcgcagg aaatgccttt
    14281 ccaactggac gtttgtacag cacaattcta ttttgtgcgg gtaagttgtt gcgtcaggag
    14341 gcgttgtgga tttctcaatc atggtttacg cagttattgc gttggtgggt gtggcaattg
    14401 gctggctgtt tgccagttat caacatgcgc agcaaaaagc cgagcaatta gctgaacgtg
    14461 aagagatggt cgcggagtta agcgcggcaa aacaacaaat tacccaaagc gagcactggc
    14521 gtgcagagtg cgagttactc aataacgaag tgcgcagcct gcaaagtatt aacacctctc
    14581 tggaggccga tctgcgtgaa gtaaccacgc ggatggaagc cgcacagcaa catgctgacg
    14641 ataaaattcg ccagatgatt aacagcgagc agcgcctcag tgagcagttt gaaaacctcg
    14701 ccaaccgtat ttttgagcac agcaatcgcc gggttgatga gcaaaaccgt cagagtctga
    14761 acagcctgtt gtcgccgcta cgtgaacaac tggacggttt ccgccgtcag gttcaggaca
    14821 gcttcggtaa agaagcacaa gaacgccata ccctgaccca cgaaattcgc aatctccagc
    14881 aactcaacgc gcaaatggcc caggaagcga tcaacctgac gcgcgcgctg aaaggcgaca
    14941 ataaaaccca gggcaactgg ggcgaggtag tattgacgcg ggtgctggag gcttccggtc
    15001 tgcgtgaagg gtatgaatat gaaacccagg tcagcatcga aaatgacgcc cgctcgcgga
    15061 tgcaaccgga tgtcatcgtg cggctgccgc agggaaaaga tgtggtgatc gacgccaaaa
    15121 tgacgctggt cgcctatgaa cgctatttca acgccgaaga tgactacacc cgcgaaagcg
    15181 cgctacagga acatatcgcg tcggtgcgta accatatccg tttgctggga cgcaaagatt
    15241 atcaacagct gccggggctg cgaactctgg attacgtgct gatgtttatt cccgttgaac
    15301 ccgctttttt actggcgctt gaccgccagc cggagctgat caccgaagcg ttgaaaaaca
    15361 atatcatgct ggttagcccg actacgctgc tggtggcgtt gcgtactatt gccaacctgt
    15421 ggcgttatga gcatcaaagc cgcaacgccc agcaaatcgc cgatcgtgcc agcaagctgt
    15481 acgacaagat gcgtttgttc atcgatgaca tgtccgcgat tggtcaaagt ctcgacaaag
    15541 cgcaggataa ctaccgccag gcaatgaaaa aactctcttc aggtcgcgga aatgtgctgg
    15601 cgcaggcaga agcgtttcgc ggtttaggag tagaaattaa acgcgagatt aatccggatt
    15661 tggctgaaca ggcggtgagc caggatgaag agtatcgact tcggtcggtt ccggagcagc
    15721 cgaatgatga agcttatcaa cgcgatgatg aatataatca gcagtcgcgc tagcccatta
    15781 ggggtagtta agccgggtag aaatctaggg catcgacgcc caatctgtta cacttctgga
    15841 acaatttttt gatgagcagg cattgagatg gtggataagt cacaagaaac gacgcacttt
    15901 ggttttcaga ccgtcgcgaa ggaacaaaaa gcggatatgg tcgcccacgt tttccattcc
    15961 gtggcatcaa aatacgatgt catgaatgat ttgatgtcat ttggtattca tcgtttgtgg
    16021 aagcgattca cgattgattg cagcggcgta cgccgtgggc agaccgtgct ggatctggct
    16081 ggtggcaccg gcgacctgac agcgaaattc tcccgcctgg tcggagaaac tggcaaagtg
    16141 gtccttgctg atatcaatga atccatgctc aaaatgggac gcgagaagct gcgtaatatc
    16201 ggtgtgattg gcaacgttga gtatgttcag gcgaacgctg aggcgctgcc gttcccggat
    16261 aacacctttg attgcatcac catttcgttt ggtctgcgta acgtcaccga caaagataaa
    16321 gcactgcgtt caatgtatcg cgtgctgaaa cccggcggcc gcctgctggt gcttgagttc
    16381 tcgaagccaa ttatcgagcc gctgagcaaa gcctatgatg catactcctt ccatgtgctg
    16441 ccgcgtattg gctcactggt cgcgaacgac gccgacagct accgttatct ggcagaatcc
    16501 atccgtatgc atcccgatca ggataccctg aaagccatga tgcaggatgc cggattcgaa
    16561 agtgtcgact actacaatct gacggcaggg gttgtggcgc tgcatcgtgg ttataagttc
    16621 tgacaggaga ccggaaatgc cttttaaacc tttagtgacg gcaggaattg aaagtctgct
    16681 caacaccttc ctgtatcgct cacctgcgct gaaaacagcc cgctcgcgtc tgctgggtaa
    16741 agtattgcgc gtggaggtaa aaggcttttc gacgtcattg attctggtgt tcagcgaacg
    16801 ccaggttgat gtactgggcg aatgggcagg cgatgctgac tgcaccgtta tcgcctacgc
    16861 cagtgtgttg ccgaaacttc gcgatcgcca gcagcttacc gcactgattc gcagtggtga
    16921 gctggaagtg cagggcgata ttcaggtggt gcaaaacttc gttgcgctgg cagatctggc
    16981 agagttcgac cctgcggaac tgctggcccc ttataccggt gatatcgccg ctgaaggaat
    17041 cagcaaagcc atgcgcggag gcgcaaagtt cctgcatcac ggcattaagc gccagcaacg
    17101 ttatgtggcg gaagccatta ctgaagagtg gcgtatggca cccggtccgc ttgaagtggc
    17161 ctggtttgcg gaagagacgg ctgccgtcga gcgtgctgtt gatgccctga ccaaacggct
    17221 ggaaaaactg gaggctaaat gacgccaggt gaagtacggc gcctatattt catcattcgc
    17281 acttttttaa gctacggact tgatgaactg atccccaaaa tgcgtatcac cctgccgcta
    17341 cggctatggc gatactcatt attctggatg ccaaatcggc ataaagacaa acttttaggt
    17401 gagcgactac gactggccct gcaagaactg gggccggttt ggatcaagtt cgggcaaatg
    17461 ttatcaaccc gccgcgatct ttttccaccg catattgccg atcagctggc gttattgcag
    17521 gacaaagttg ctccgtttga tggcaagctg gcgaagcagc agattgaagc tgcaatgggc
    17581 ggcttgccgg tagaagcgtg gtttgacgat tttgaaatca agccgctggc ttctgcttct
    17641 atcgcccagg ttcataccgc gcgattgaaa tcgaatggta aagaggtggt gattaaagtc
    17701 atccgcccgg atattttgcc ggtcattaaa gcggatctga aacttatcta ccgtctggct
    17761 cgctgggtgc cgcgtttgct gccggatggt cgccgtctgc gcccaaccga agtggtgcgc
    17821 gagtacgaaa agaccttgat tgatgaactg aatttgctgc gggaatctgc caacgccatt
    17881 cagcttcggc gcaattttga agacagcccg atgctctaca tcccggaagt ttaccctgac
    17941 tattgtagtg aagggatgat ggtgatggag cgcatttacg gcattccggt gtctgatgtt
    18001 gcggcgctgg agaaaaacgg cactaacatg aaattgctgg cggaacgcgg cgtgcaggtg
    18061 ttcttcactc aggtctttcg cgacagcttt ttccatgccg atatgcaccc tggcaacatc
    18121 ttcgtaagct atgaacaccc ggaaaacccg aaatatatcg gcattgattg cgggattgtt
    18181 ggctcgctaa acaaagaaga taaacgctat ctggcagaaa actttatcgc cttctttaat
    18241 cgcgactatc gcaaagtggc agagctacac gtcgattctg gctgggtgcc accagatacc
    18301 aacgttgaag agttcgaatt tgccattcgt acggtctgtg aacctatctt tgagaaaccg
    18361 ctggccgaaa tttcgtttgg acatgtactg ttaaatctgt ttaatacggc gcgtcgcttc
    18421 aatatggaag tgcagccgca actggtgtta ctccagaaaa ccctgctcta tgtcgaaggg
    18481 gtaggacgcc agctttatcc gcagctcgat ttatggaaaa cagcgaagcc tttcctggag
    18541 tcgtggatta aagatcaggt cggtattcct gcgctggtga gagcatttaa agaaaaagcg
    18601 ccgttctggg tcgaaaaaat gccagaactg cctgaattgg tttacgacag tttgcgccag
    18661 ggcaagtatt tacagcacag tgttgataag attgcccgcg agcttcagtc aaatcatgta
    18721 cgtcagggac aatcgcgtta ttttctcgga attggcgcta cgttagtatt aagtggcaca
    18781 ttcttgttgg tcagccgacc tgagtggggg ctgatgcccg gctggttaat ggcaggtggt
    18841 ctgatcgcct ggtttgtcgg ttggcgcaaa acacgctgat tttttcatcg ctcaaggcgg
    18901 gccgtgtaac gtataatgcg gctttgttta atcatcatct accacagagg aacatgtatg
    18961 ggtggtatca gtatttggca gttattgatt attgccgtca tcgttgtact gctttttggc
    19021 accaaaaagc tcggctccat cggttccgat cttggtgcgt cgatcaaagg ctttaaaaaa
    19081 gcaatgagcg atgatgaacc aaagcaggat aaaaccagtc aggatgctga ttttactgcg
    19141 aaaactatcg ccgataagca ggcggatacg aatcaggaac aggctaaaac agaagacgcg
    19201 aagcgccacg ataaagagca ggtgtaatcc gtgtttgata tcggttttag cgaactgcta
    19261 ttggtgttca tcatcggcct cgtcgttctg gggccgcaac gactgcctgt ggcggtaaaa
    19321 acggtagcgg gctggattcg cgcgttgcgt tcactggcga caacggtgca gaacgaactg
    19381 acccaggagt taaaactcca ggagtttcag gacagtctga aaaaggttga aaaggcgagc
    19441 ctcactaacc tgacgcccga actgaaagcg tcgatggatg aactacgcca ggccgcggag
    19501 tcgatgaagc gttcctacgt tgcaaacgat cctgaaaagg cgagcgatga agcgcacacc
    19561 atccataacc cggtggtgaa agataatgaa gctgcgcatg agggcgtaac gcctgccgct
    19621 gcacaaacgc aggccagttc gccggaacag aagccagaaa ccacgccaga gccggtggta
    19681 aaacctgctg cggacgctga accgaaaacc gctgcacctt ccccttcgtc gagtgataaa
    19741 ccgtaaacat gtctgtagaa gatactcaac cgcttatcac gcatctgatt gagctgcgta
    19801 agcgtctgct gaactgcatt atcgcggtga tcgtgatatt cctgtgtctg gtctatttcg
    19861 ccaatgatat ctatcacctg gtatccgcgc cattgatcaa gcagttgccg caaggttcaa
    19921 cgatgatcgc caccgacgtg gcctcgccgt tctttacgcc gatcaagctg acctttatgg
    19981 tgtcgctgat tctgtcagcg ccggtgattc tctatcaggt gtgggcattt atcgccccag
    20041 cgctgtataa gcatgaacgt cgcctggtgg tgccgctgct ggtttccagc tctctgctgt
    20101 tttatatcgg tatggcattc gcctactttg tggtctttcc gctggcattt ggcttccttg
    20161 ccaataccgc gccggaaggg gtgcaggtat ccaccgacat cgccagctat ttaagcttcg
    20221 ttatggcgct gtttatggcg tttggtgtct cctttgaagt gccggtggca attgtgctgc
    20281 tgtgctggat ggggattacc tcgccagaag acttacgcaa aaaacgcccg tatgtgctgg
    20341 ttggtgcatt cgttgtcggg atgttgctga cgccgccgga tgtcttctcg caaacgctat
    20401 tggcgatccc gatgtactgt ctgtttgaaa tcggtgtctt cttctcacgc ttttacgttg
    20461 gtaaagggcg aaatcgggaa gaggaaaacg acgctgaagc agaaagcgaa aaaactgaag
    20521 aataaattca accgcccgtc agggcggttg tcatatggag tacaggatgt ttgatatcgg
    20581 cgttaatttg accagttcgc aatttgcgaa agaccgtgat gatgttgtag cgcgcgcttt
    20641 tgacgcggga gttaatgggc tactcatcac cggcactaac ctgcgtgaaa gccagcaggc
    20701 gcaaaagctg gcgcgtcagt attcgtcctg ttggtcaacg gcgggcgtac atcctcacga
    20761 cagcagccag tggcaagctg cgactgaaga agcgattatt gagctggccg cgcagccaga
    20821 agtggtggcg attggtgaat gtggtctcga ctttaaccgc aacttttcga cgccggaaga
    20881 gcaggaacgc gcttttgttg ctcagctacg cattgccgca gaattaaaca tgccggtatt
    20941 tatgcactgt cgcgatgccc acgagcggtt tatgacattg ctggagccgt ggctggataa
    21001 actgcctggt gcggttcttc attgctttac cggcacacgc gaagagatgc aggcgtgcgt
    21061 ggcgcgtgga atttatatcg gcattaccgg ttgggtttgc gatgaacgac gcgggctgga
    21121 gctgcgggaa ttgttgccgt tgattccggc ggagaaattg ctgatcgaaa ctgatgcgcc
    21181 gtatctgctc ccgcgcgatc tcacgccaaa gccatcatcc cggcgcaacg agccagccca
    21241 tctgccccat attttgcaac gtattgcgca ctggcgtgga gaagatgccg catggctggc
    21301 tgccaccacg gatgccaatg tcaaaacact gtttgggatt gcgttttaga gtttgcggaa
    21361 ctcggtgttc ttcacactgt gcttaatctc tttattaata agattaagca atagcatgga
    21421 gcgagcctca ccatcgggtt cggtgaaaat ggcctgaaag ccttcgaacg cgccttcggt
    21481 aataatcacc ttatcacccg gataaggggt tgccggatcg acaatgtctt tcggtttata
    21541 taccgatagc tgatgaatca ccgccgatgg gactatcgct ggcgacgcgc caaagcgcac
    21601 gaagtggctg acaccgcggg tcgcgttgat agtcgtggta tgaatcactt ctggatcaaa
    21661 ttcgacaaac aggtagttgg gaaacaatgg ctcactgact gcagtacgtt ttccacgcac
    21721 gattttttcc agggtgatca tcggtgccag gcaattcaca gcctgtcttt cgaggtgttc
    21781 ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa taccaggatt gcataatgac
    21841 tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt tacgctaagt taattatatt
    21901 ccccggtttg cgttataccg tcagagttca cgctaattta acaaatttac agcatcgcaa
    21961 agatgaacgc cgtataatgg gcgtagatta agaggctaca atggacgcca tgaaatataa
    22021 cgatttacgc gacttcctga cactgcttga acagcagggt gagctaaaac gtatcacgct
    22081 cccggtggat ccgcatctgg aaatcactga aattgctgac cgcactttgc gtgccggtgg
    22141 gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg ccggtgctgt gcaacctgtt
    22201 cggtacgcca aagcgcgtgg caatgggcat ggggcaggaa gatgtttcgg cgctacgtga
    22261 agttggtaaa ttattggcgt ttctgaaaga gccggagccg ccaaaaggtt tccgcgacct
    22321 gtttgataaa ctgccgcagt ttaagcaagt attgaacatg ccgacaaagc ggctgcgtgg
    22381 tgcgccctgc caacaaaaaa tcgtctctgg cgatgacgtc gatctcaatc gcattcccat
    22441 tatgacctgc tggccggaag atgccgcgcc gctgattacc tgggggctga cagtgacgcg
    22501 cggcccgcat aaagagcggc agaatctggg catttatcgc cagcagctga ttggtaaaaa
    22561 caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg ctggattatc aggagtggtg
    22621 tgcggcgcat ccgggcgaac gtttcccggt ttctgtggcg ctgggtgccg atcccgccac
    22681 gattctcggt gcagtcaccc ccgttccgga tacactttca gagtatgcgt ttgccggatt
    22741 gctgcgcggt accaagaccg aagtggtgaa gtgtatctcc aatgaccttg aagtgcccgc
    22801 cagtgcggag attgtgctgg aagggtatat cgaacaaggt gaaacggcgc cggaagggcc
    22861 gtatggcgac cacaccggtt actataacga agtcgatagt ttcccggtat ttaccgtgac
    22921 gcatattacc cagcgtgaag atgcgattta ccactccacc tatactggtc gtccgccgga
    22981 tgaacccgcg gtgctgggtg tcgcactgaa cgaagtgttt gtgccgattc tgcaaaaaca
    23041 gttcccggaa attgtcgatt tttacctgcc gccggaaggc tgttcttatc gtctggcggt
    23101 agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc gtcatgatgg gcgtctggtc
    23161 gttcttacgc cagtttatgt acactaaatt tgtgatcgtt tgcgatgatg acgtcaacgc
    23221 acgcgactgg aacgatgtga tttgggcgat taccacccgt atggacccgg cgcgggatac
    23281 tgttctggta gaaaatacgc ctattgatta tctggatttt gcctcgcctg tctccgggct
    23341 gggttcaaaa atggggctgg atgccacgaa taaatggccg ggggaaaccc agcgtgaatg
    23401 gggacgtccc atcaaaaaag atccagatgt tgtcgcgcat attgacgcca tctgggatga
    23461 actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg tttgttttgc cctatttatc
    23521 gatccgacag agaaagcgca tgacaacctt aagctgtaaa gtgacctcgg tagaagctat
    23581 cacggatacc gtatatcgtg tccgcatcgt gccagatgcg gccttttctt ttcgcgctgg
    23641 tcagtatttg atggtagtga tggatgagcg cgacaaacgt ccgttctcaa tggcttcgac
    23701 gccggatgaa aaagggttta tcgagctgca tattggcgct tctgaaatca acctttacgc
    23761 gaaagcagtc atggaccgca tcctcaaaga tcatcaaatc gtggtcgata ttccccacgg
    23821 agaagcgtgg ctgcgcgatg atgaagagcg tccgatgatt ttgattgcgg gcggcaccgg
    23881 gttctcttat gcccgctcga ttttgctgac agcgttggcg cgtaacccaa accgtgatat
    23941 caccatttac tggggcgggc gtgaagagca gcatctgtat gatctctgcg agcttgaggc
    24001 gctttcgttg aagcatcctg gtctgcaagt ggtgccggtg gttgaacaac cggaagcggg
    24061 ctggcgtggg cgtactggca ccgtgttaac ggcggtattg caggatcacg gtacgctggc
    24121 agagcatgat atctatattg ccggacgttt tgagatggcg aaaattgccc gcgatctgtt
    24181 ttgcagtgag cgtaatgcgc gggaagatcg cctgtttggc gatgcgtttg catttatctg
    24241 agatataaaa aaacccgccc ctgacaggcg ggaagaacgg caactaaaac tttattcagt
    24301 ggcatttaga tctatgtcgc atccggcatt cggcacacga
//
LOCUS       DAZFVO020000067        24036 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFVO020000067 DAZFVO020000000
VERSION     DAZFVO020000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.067"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            53..571
                     /gene="yfgH"
                     /locus_tag="ACHKNB_RS12435"
                     /old_locus_tag="pgaptmp_002487"
     CDS             53..571
                     /gene="yfgH"
                     /locus_tag="ACHKNB_RS12435"
                     /old_locus_tag="pgaptmp_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HGU4195982.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITRTYNET
                     RIQPNTKCPEKS"
     gene            587..1126
                     /gene="yfgI"
                     /locus_tag="ACHKNB_RS12440"
                     /old_locus_tag="pgaptmp_002488"
     CDS             587..1126
                     /gene="yfgI"
                     /locus_tag="ACHKNB_RS12440"
                     /old_locus_tag="pgaptmp_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HGU4195983.1"
                     /translation="MKKVFLCAILASLCYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKNB_RS12445"
                     /old_locus_tag="pgaptmp_002489"
     CDS             complement(1221..2798)
                     /gene="guaA"
                     /locus_tag="ACHKNB_RS12445"
                     /old_locus_tag="pgaptmp_002489"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGU4195984.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKNB_RS12450"
                     /old_locus_tag="pgaptmp_002490"
     CDS             complement(2867..4333)
                     /gene="guaB"
                     /locus_tag="ACHKNB_RS12450"
                     /old_locus_tag="pgaptmp_002490"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGU4195985.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKNB_RS12455"
                     /old_locus_tag="pgaptmp_002491"
     CDS             4495..5865
                     /gene="xseA"
                     /locus_tag="ACHKNB_RS12455"
                     /old_locus_tag="pgaptmp_002491"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGU4195986.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5862..6077)
                     /gene="yfgJ"
                     /locus_tag="ACHKNB_RS12460"
                     /old_locus_tag="pgaptmp_002492"
     CDS             complement(5862..6077)
                     /gene="yfgJ"
                     /locus_tag="ACHKNB_RS12460"
                     /old_locus_tag="pgaptmp_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HGU4195987.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKNB_RS12465"
                     /old_locus_tag="pgaptmp_002493"
     CDS             complement(6146..7618)
                     /gene="der"
                     /locus_tag="ACHKNB_RS12465"
                     /old_locus_tag="pgaptmp_002493"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGU4195988.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKNB_RS12470"
                     /old_locus_tag="pgaptmp_002494"
     CDS             complement(7736..8914)
                     /gene="bamB"
                     /locus_tag="ACHKNB_RS12470"
                     /old_locus_tag="pgaptmp_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HGU4195989.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKNB_RS12475"
                     /old_locus_tag="pgaptmp_002495"
     CDS             complement(8925..9545)
                     /gene="yfgM"
                     /locus_tag="ACHKNB_RS12475"
                     /old_locus_tag="pgaptmp_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HGU4195990.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKNB_RS12480"
                     /old_locus_tag="pgaptmp_002496"
     CDS             complement(9563..10837)
                     /gene="hisS"
                     /locus_tag="ACHKNB_RS12480"
                     /old_locus_tag="pgaptmp_002496"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGU4195991.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKNB_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /gene_synonym="gcpE"
     CDS             complement(10948..12066)
                     /gene="ispG"
                     /locus_tag="ACHKNB_RS12485"
                     /old_locus_tag="pgaptmp_002497"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HGU4195992.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKNB_RS12490"
                     /old_locus_tag="pgaptmp_002498"
     CDS             complement(12093..13106)
                     /gene="rodZ"
                     /locus_tag="ACHKNB_RS12490"
                     /old_locus_tag="pgaptmp_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGU4195993.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13391..14545)
                     /gene="rlmN"
                     /locus_tag="ACHKNB_RS12495"
                     /old_locus_tag="pgaptmp_002499"
     CDS             complement(13391..14545)
                     /gene="rlmN"
                     /locus_tag="ACHKNB_RS12495"
                     /old_locus_tag="pgaptmp_002499"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HGU4195994.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKNB_RS12500"
                     /old_locus_tag="pgaptmp_002500"
     CDS             complement(14695..15126)
                     /gene="ndk"
                     /locus_tag="ACHKNB_RS12500"
                     /old_locus_tag="pgaptmp_002500"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGU4195995.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKNB_RS12505"
                     /old_locus_tag="pgaptmp_002501"
     CDS             complement(15275..17587)
                     /gene="pbpC"
                     /locus_tag="ACHKNB_RS12505"
                     /old_locus_tag="pgaptmp_002501"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HGU4195996.1"
                     /translation="MPRLLTKRGYWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKNB_RS12510"
                     /old_locus_tag="pgaptmp_002502"
     CDS             complement(17588..22549)
                     /gene="yfhM"
                     /locus_tag="ACHKNB_RS12510"
                     /old_locus_tag="pgaptmp_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HGU4195997.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGTAEDLLIVRP"
     gene            22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKNB_RS12515"
                     /old_locus_tag="pgaptmp_002503"
     CDS             22756..23601
                     /gene="sseA"
                     /locus_tag="ACHKNB_RS12515"
                     /old_locus_tag="pgaptmp_002503"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HGU4195998.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23937..>24036
                     /gene="timP"
                     /locus_tag="ACHKNB_RS12520"
                     /old_locus_tag="pgaptmp_002504"
     CDS             23937..>24036
                     /gene="timP"
                     /locus_tag="ACHKNB_RS12520"
                     /old_locus_tag="pgaptmp_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HGU4195999.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLV"
BASE COUNT         5723 a         6637 c         6001 g         5675 t
ORIGIN      
        1 cttttgatga tcttaaatgt cttatttttc gtaatgtgta taacaaggaa tagtgatgaa
       61 atttaaaaaa tgtcttctgc ctgtggcaat gttagcgtca ttcactctgg caggatgcca
      121 gtcaaatgct gacgatcatg ccgccgatgt ttatcaaacc gatcaactga ataccaaaca
      181 agaaactaaa accgttaata ttatttccat tcttcccgca aaagttgccg tagacaactc
      241 ccaaaataaa cggaacgcac aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt
      301 tatcggccac aacgtcggtt ctggcagcaa ttccggaacg acggcagggg cagttggcgg
      361 cggagctgta ggcgcggcag tgggttctat ggtgaatgat aaaaccttag tggaaggtgt
      421 ttctttaaca tataaggaag gcaccaaagt gtatacctct acccaggtgg gtaaagagtg
      481 ccagtttacg acaggtttag ccgttgttat taccagaacg tataacgaaa cgcgtattca
      541 gccaaatact aaatgtcctg aaaagagcta ataatcagga ggagtcatga agaaagtttt
      601 tctttgcgcc atcttggcct ccttatgcta tccggctatc gcctcatcat tgcaggatca
      661 actctctgct gtagcagaag ctgaacagca aggtaaaaat gaagagcaaa ggcagcatga
      721 cgaatgggtc gcggagcgca acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc
      781 ccaggccgcc gctaacaaaa gagcggcaac ggcagcggca aataagaaag ctcgtcagga
      841 taaactggac gccgaagcct ctgcggacaa aaaacgcgat caaagttatg aagatgagct
      901 acgcagctta gagattcaga aacaaaaact ggcgctggca aaagaagaag cccgcgttaa
      961 gcgagaaaac gaatttatcg atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt
     1021 gcaatctgaa gctgacgcca acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag
     1081 cgtgggcaaa gcagaagaga acaaatcgga cagctggttt aattaatcga tgttagtaac
     1141 ttcaagccta tgattcttga agataaaaaa ccctctgtag taacagaggg ttttgttcat
     1201 tcatagtgca gggattaaaa tcattcccac tcaatggtag ctggcggctt gccgctgatg
     1261 tcatacacca cgcgggaaat accgttcact tcattgataa tgcggttgga aacgcgaccg
     1321 aggaaatcgt acggcagatg cgcccagtgt gcggtcataa agtcgatggt ttcgacagca
     1381 cgcagagaga caacccagtc atacttacga ccatcgccca ttacgccaac ggaacgtacc
     1441 ggcaggaaca cagtgaacgc ctggctgact ttgtcgtaca ggtccgcttt acgcagttct
     1501 tcaatgaaga tggcgtcagc acggcgcagc aggtcacagt actctttctt cacttcaccc
     1561 agcacacgaa cgccaaggcc tggtcccggg aacgggtgac ggtacagcat gtcgtacggc
     1621 aggcccagct ccagaccaat cttacgcact tcgtctttga acagctcttt cagcggttca
     1681 accaggccca tcttcatctc tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg
     1741 tgtgctttac cggttgcaga agccgcagat tcgataacgt cagggtagat ggtgccctgc
     1801 gccagccact tcacgtcttc cagtttcagc gcttcttcat cgaatacttc aacgaaaacg
     1861 cgcccgatga ttttacgttt tgcttccgga tcgttttcgc cagccagcgc tgacaggaag
     1921 cgatcttctg ccggtacgtg aacaatgtta agaccaaagt gatcgccaaa catatccaga
     1981 acctgctctg cttcgttgag acgcagcagg ccgttgtcga cgaatacgca agtcaggttt
     2041 ttaccgatag cgcggtgcag cagcattgcg gttacggagg aatccacacc accagagagg
     2101 ccgaggatga ctttatcgtc gcctacctgc tcgcggatgc gagctacagc atcgtcgata
     2161 attttcgctg gcgtccacag ggcttcacac tggcagatat cacgcacaaa acgctccagc
     2221 atgcgcatac cctggcgggt gtgagtcact tccgggtgga actgtacgcc atagaagcgt
     2281 ttttcttcgt tagccataat ggcaaacggg cagctttcgg tgctggctac ggtgacgaag
     2341 tcggacggga tagcggtaac tttatcgccg tggctcatcc agacatcgag cagcggttta
     2401 ccgtctgcgg tcagcgcatc ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca
     2461 acctgcgcgt agccaaattc acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt
     2521 gccatggttt gcatgccata gcaaacgccg aataccggta cgcctgcttc aaagacatac
     2581 tgcggcgcac gcgggctgtt ttcttcggtg gtgctttccg ggccgccgga aagaataatg
     2641 ccgcttggat tgaagtcacg aatttgtgct tctgtcacat cccacgccca cagttcgcag
     2701 taaacaccca gctcacgcac gcggcgcgca accagttgag tgtactgaga accgaagtcc
     2761 agaatgagga tgcgatgctt atgaatgttt tccgtcattg acgcttattc cgaggcaagt
     2821 gaaacagata atataaatcg cccgacatga agtcgggcga agagaatcag gagcccagac
     2881 ggtagttcgg ggactcttta gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg
     2941 caccgctgat acgtacaaac tccgctttag tacgcagttc gtcgatagta ccacagccgg
     3001 tcagacccat acaggagcgc aggccgccca tctgctggtg aatgatctct ttcaggcgac
     3061 ctttataagc tacgcgacct tcgatacctt ccggcaccag tttgtcggca gcgttatcgc
     3121 tctggaaata acggtcagag gaacctttgg acatcgcgcc cagggaaccc ataccacggt
     3181 aagatttgta agaacggccc tggtagagtt cgatttcacc cggagattct tcagtacccg
     3241 ccagcatgga acccaccatt accgcgcttg cgccagcggc gatagctttg gcgatgtcgc
     3301 cggagaagcg aataccacca tctgcgataa ccggaatacc ggtgccttcc agggcttcaa
     3361 ctgcgtcagc aacggcagtg atctgcggaa cacctacgcc agtaacgata cgagtcgtac
     3421 agatagaacc agggccgata ccgactttaa ccgcgctgca accagcttct gccaaagcgc
     3481 gtgcacctgc agctgttgcc acgttgccgc cgatgatttg cagatccgga tatttagcac
     3541 gggtttcacg gatacgctgc aaaacgcctt ctgagtgacc gtgggaggag tcgatcagca
     3601 gaacgtcaac gccagcggca accagcgcgt caacacgctc ttcgttaccc gcacctgcgc
     3661 caactgcagc accaacacgc agacggcctt gctcgtcttt acaggcgttc ggtttacgtt
     3721 ccgctttctg gaagtctttc acggtgatca tgccgatcag gtggaattcg tcatcaacca
     3781 ccagcgcttt ttcaacgcgt ttttcgtgca tttttgccag caccacttca cgggcttcac
     3841 cttcacgcac ggtgaccaga cgctctttcg gcgtcatgta aacgctaacc ggctggttca
     3901 ggtcggtaac aaaacgcacg tcacgaccgg tgataatacc caccagttcg ttttcttcgg
     3961 taacgaccgg atagcccgca aaaccgttac gctcagtcag ttctttcact tcgcgcagcg
     4021 tcgtggttgg cagaacagtc tgcggatcag tcaccacacc agattcgtgt tttttcacac
     4081 ggcgaacttc ttctgcctgg cgttcaatgg acatgttttt gtggataaag ccgataccgc
     4141 cttcctgagc cagagcaata gccaggcgcg cttccgttac ggtatccatt gctgcggaaa
     4201 gcatagggat attcagacga atagttttcg tcagctgggt gctgaggtca gcagtattcg
     4261 gcagaacggt agaatgagca ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag
     4321 cgatacgtag catgggcaat atctcgacca gagtggttaa taaatattgc cgcggcatta
     4381 tacagagcgt aaccgattgc atctacccct ttttgcaaaa aatgcttgct atccccgaag
     4441 ggcgggttac tatcgactga ataacctgct gatttagaat ttgatctcgc tcacatgtta
     4501 ccttctcaat cccctgcaat ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg
     4561 cttgagcatg agatgggaca ggtttggatc agcggcgaaa tctctaattt cacacaaccg
     4621 gcttccggtc actggtactt tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg
     4681 ttccgcaaca gcaaccgccg ggtgaccttc cgtccacagc atggacaaca agttttagtt
     4741 cgcgccaata ttacgctcta cgagccgcgc ggtgactacc agataatcgt tgagagtatg
     4801 cagccggccg gtgaagggct gctgcaacag aagtacgaac agctcaaagc gaagttgcag
     4861 gctgaaggtt tgttcgatca gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt
     4921 ggtgtgatca cctcaaaaac cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt
     4981 cgcgatcctt ctctaccggt gatcatctac cccaccgccg ttcagggcga tgacgcaccg
     5041 gggcaaattg ttcgcgccat tgaactggcg aatcagcgca atgagtgcga cgtgttgatc
     5101 gttgggcgcg gcggcggttc gctggaagat ttatggagtt ttaacgacga acgcgtagcg
     5161 cgggcgattt ttgccagccg cattccggtc gtcagcgccg tcgggcatga gacggatgtg
     5221 accattgccg attttgttgc cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta
     5281 gtgagccgta atcagcaaga gttactgcgc caggtgcaat cgacccgtca acggctggag
     5341 atggcgatgg attattatct cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga
     5401 ttacagcaac agcatccgca gctccggctg gcacgccagc aaaccatgct tgagcgcctg
     5461 caaaagcgga tgagctttgc gctggaaaat caacttaagc gtaccgggca acagcagcag
     5521 cggttaacac agcggctgaa tcagcaaaat ccacagccga agattcatcg cgcgcaaacg
     5581 cgcattcagc aactggaata tcgtttagca gaaaccctgc gcgtacagct tagcgccacg
     5641 cgtgaacgtt tcggtaatgc agtaacgcac ctcgaagccg taagcccact gtcaacgctg
     5701 gcgcgtggat acagcgttac tactgctact gacggcaatg tactgaaaaa agtgaagcaa
     5761 gttaaagcgg gtgaaatgct aaccacacgt ctggaagacg gctggataga aagtgaagtt
     5821 aaaaacatcc agccagtaaa aaaatcgcgt aaaaaggtgc attaagccag cacgaattca
     5881 acgcgttttt tcgatatcaa gccatgcccg tgctgacaga agtaatctac tgcaccgcag
     5941 gcttttaaca cctgcaacgg ttgatggcag tctggacaaa gagctttcat ttcaataatt
     6001 tccccgcagg atgggcaacg cgcatggtcg ttatcctgat caagcacatg ctgacattgt
     6061 ggacaatgca gttccatatg aattcctccg gtagccatcc ctctacattc atagagggaa
     6121 tggcagataa aatacttacg gataattatt tatttttctt gatgtgcttc atcagacgct
     6181 tacgtttacg catctgggtt ggcgtcaggg tgttacgctt gttcgcatac gggttttccc
     6241 cttctttgaa ctgaatacga atcggcgatc ccattacgtc cagcgatttg cggaagtagt
     6301 tcatcaagta gcgcttgtag gaatcaggca ggtctttcac ctgattaccg tgaatcacca
     6361 caatcggcgg gttataacca ccagcgtggg catatttcag cttcacacga cgaccgcgta
     6421 ccagcggcgg ttggtgatct tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc
     6481 ccacacgacg ggtggagctg tcatacgctt cacgtactga ttcaaacaag ttaccaacac
     6541 cactgccgtg caaggcagag ataaagtgca cacgagcaaa atcgataaag cccagacgga
     6601 agtccagcgt ttctttcacc tgctctttca cttcctggct caggccatcc cacttattca
     6661 ccacaatgac aagtgagcgc ccactattga gaataaagcc cagcagcgag agatcctgat
     6721 cggaaatacc ttcgcgcgca tcaatcacta acatcaccac gttggcatct tcaatggcct
     6781 gcaacgtttt gattacggag aatttctcta cagcatcggt gattttgccg cgtttacgta
     6841 cgccagcggt gtcaatgagc acatactcac gtccatcgcg ttccattgga atgtagatac
     6901 tgtcacgcgt cgtgccaggc atgtcataaa caacaacgcg ctcttcacca agaatacggt
     6961 tagtgagtgt agacttacct acgttcggac gacccacaat cgccagtttg atcggcagac
     7021 tttgcgggtc gaagtcgtct tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg
     7081 cccagtattc agcgtcttcg tcgacttcct cttgcggtgc gagatcttcc atccacggca
     7141 gcagcacatg ctccagcaga cttaatacgc cacgaccgtg agacgcggcg atcgggtaga
     7201 tttcacctaa accaagcgag tagaaatcaa ccactgcctg atcgggatcc agaccgtcag
     7261 ttttgtttgc caccaggaag gtcggttttt cacgggagcg cagatgtttg gcaatcgctt
     7321 catctgccgg catcaggccc gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt
     7381 caatcgccag cagcgactgt tccgccatgc gggtttctac accgtcttct gtgccatcaa
     7441 tcccgccggt atcaatacag ataaactcac ggccttcaat ttccgcacga ccgtacttac
     7501 ggtcacgagt cagacccggg aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt
     7561 taaataacgt ggattttcct acgttagggc gcccgacaag cgcgaccaca ggtaccatgt
     7621 ttaaagcctc atttttataa atcatcagac aacgcacgct atattcgcgt cgttgttaaa
     7681 aacaggaaaa cggcccctgt ccaggagccg ttttcaaagt gaacgacaga gacgattaac
     7741 gtgtaataga gtacacggtt ccgtcttttg cctggatcag cagtttgccg tcagcggcaa
     7801 ccggttcagt ctggaaaccg gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt
     7861 cgacgttaat ccagtgcaga taaccttcac tgtcaccgac caccaggttg ccattataca
     7921 gcaccggaga agtcagcagg cgatgcagca gatcgctttg tgcccacagc gtaacgccgc
     7981 catcaatggt caacgccatc acccggtcat tttgatcgac cagatagatg cgattgccgt
     8041 cgacgatgaa atcattcacc gaacccagtt cgcgtttcca cataatctga ccactgcgca
     8101 gatcaagcgc cgtcaggtta ccattatagg ccagcgcgaa aacaacgccg ttaacaacga
     8161 cgggagtcgt gtcaacatcg ctcagacggt caatttcggt agaaccggtc gcctgggaaa
     8221 tacgctgctg ccaaatcatc tggccctgtt ccatcagcac tgcgctgacg cgaccattat
     8281 cgccccccac gacggccgca ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga
     8341 gcgaaggcat atcgaggtta actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt
     8401 gtaactgacc gttactggtg tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa
     8461 gtgcttcacc cgcgacttta gtttgccatg ccacagtacc atcgctggta ttcagcgcgt
     8521 aaacctgcgc cttttcgctg ccaatgtaga catgcccacc agacacggtc acaccgccag
     8581 aaagtaatgc aggctcttta gagaaccagc catctttctc ggccaagctg acagaccaga
     8641 tttctttgcc atcatccgca ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga
     8701 caacgttgtc cgccagtgcc ggatgaagat tggaatagaa gttgccaatg ccgctaccaa
     8761 cggaagtact ccacgccgtg gtcggcgtaa actggttttc aacggttggc aatggggaca
     8821 tctttaccac atcttcttcg ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa
     8881 gcagtcctgg cagcagtaat ttacgcaatt gcatcgggtc cctctcagat ggacaaatta
     8941 ttaattttca tctgcatcat ttcgctcagt gccggagtaa catcgctttt cacgcctgct
     9001 tcccatgcac tacgcgcacc ttgcttatca cctttgctca gcaatgcttc accacgcagg
     9061 tcggcaacaa tggcagccca cccttcacct ttgatggtat caagggtttt cagcgcggca
     9121 tcagcctgct tgagctgtac ctgaacgcga gcaagacgca gatttatcac ggctttgaga
     9181 ttttcatcgc tcgtgtctgc cagcccctgt tgtaactggg cggcagcttt ctctagttca
     9241 tttttgtcaa caaattgctg cgcaagttcc aaagaagcca gcgcaccata agtattttta
     9301 ttttcagcag caaatttttc cgccgccggg atgctatccg gtttgccttc gctcactgcg
     9361 gtaaccgcat tttgataggc aagagaagcg gagcgtgcag aatcaacctg atggctgttc
     9421 cagtagcgcc agccaatcag tgcgccaacg cccaaaatca ccccaacagc cagtgctttg
     9481 ccattttcag caaaaaagcg tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt
     9541 tccacgctgt ccttctcctt ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat
     9601 cctgcgcaac tgccgtttgc tcaccagagc gcaaatcctt cactactgct gtgccgttag
     9661 ccacttcaga ctcacccagc accacagcaa cgcgggcacc ccatttatca gcacgggcaa
     9721 actgtttctt aaagttgccg ccgccgtggt tggtcatcaa tttcacgccc ggtaattcat
     9781 cacgcagacg ctcagctaat gccatagccg cagattgtgt atcagcacct gaagccacca
     9841 ggtatatatc gacaacagga tcggctttaa attccggatt aacggcctgt actaacaata
     9901 caagacgttc gaggcccatc gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt
     9961 ccacaagacc gtcataacga ccgcctgcac acacggtgcc ctgggagccg agactgttag
    10021 tcacccactc gaaaacggta cggttatagt aatccagacc acgcaccaga cgctggttta
    10081 cggtgtaagc gatccccgcg ctctcaagca gtttgcacag accggcaaaa tgctcacgag
    10141 attcctcgtc cagataatca cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg
    10201 gattttttga atccagcacg cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt
    10261 ccagcttttc tttatgctgc tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg
    10321 cttccagcga accgatagaa ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc
    10381 gccaccagcg ggcggtgagc ataatcagtt cagcgtcgat atccggacct tgcagaccga
    10441 aaacttcgca gcccaactga tggaactgac gataacgccc tttctgcgga cgctcgtgac
    10501 ggaacatcgg cccgatatac cacagacgct gttcctgatt gtacagaaga ccatgctcga
    10561 tgccggcgcg tacacagccc gccgtccctt cagggcgcag agtcaggctg tcgccattgc
    10621 gatcctcaaa ggtgtacatc tctttttcaa ccacgtcggt gacttcacca atcgcacgtt
    10681 tgaatagcgg ggtctgctct acaatcggca agcggatttc actgtaaccg tagctgccga
    10741 gcacgttttt cagtgtgcct tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat
    10801 cgttcatgcc gcgaatggct tgaatgtttt ttgccacgtt tattctcttt ctgaatataa
    10861 aaatgaaccc tcaacgcttc cctcaatgtt tcgggagcta tgcgggttca atcatacacg
    10921 ggaagcgagg cgcttcccat cacgttatta tttttcaacc tgctgaacgt caattcgacg
    10981 cgcttcgtcc agctgactgg ctttcgcacg aatgcgtgct tccagctggt cgatcatatc
    11041 gttgttgtcc agacggtctt tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc
    11101 gccggtgaca ccgagtgtag aaaccagcgc ctcacctggg ccattcacca cgcagccgat
    11161 aatcgaaacg tccatcggag tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt
    11221 accgataaca tcaaattcct gacgcgaaca ggtcgggcag gcgatgaagt tgatcccgcg
    11281 cgaacggata cgcagcgatt tcaaaatatc gaaaccgact ttgatctctt cgaccggatc
    11341 ggccgccagt gatacgcgca gcgtgtcgcc gatgccttca gacagcagca gacctaaacc
    11401 aatggcggat tttactgccc cgctgcgtgc accaccggct tcggtgatcc ccagatgcag
    11461 cggctgatcg atctgttttg ccagcaaacg ataagactca acagcgagga agacgtcaga
    11521 cgctttcacg ctgactttga actgatcgaa gttcaggcga tcgagatgat caacatggcg
    11581 catggcggat tccagcaacg cctgcggcgt cggttcgcca tacttttctt gcagatcttt
    11641 ttccagcgat ccggcgttaa cgccaatacg gatcggaatg tttttatcgc gcgcacagtc
    11701 aaccaccatg cgaatacgct cttcattacc gatattgcca gggttaatac gcagacaatc
    11761 gacgccgtat tccgctactt tcagtgcaat gcgatagtcg aagtggatgt cagccaccag
    11821 cggcacgtta acctgctgtt tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac
    11881 ggagacacgg acgatatcag cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc
    11941 ttcgacgtct gtcgtacgcg tattggtcat ggactgtacg gcgatgggag caccatcgcc
    12001 aatcggcaca ttcccaacgt aaatacgtgt tgattttcta cgttgaattg gagcctggtt
    12061 atgcatgaaa aatctcccgc gttacccgtc tgttactgcg ccggtgattg ttcggcattg
    12121 agggtcagac gcgcaacctg gttagttctg ataaaacgac tcagatcgac aggtttccct
    12181 tgatactgga tctgtactgc ggctggcgca ccaattttca gtttgtatgg tgcctggccg
    12241 gttaagttca aattaccgtc tttacgctgc ataccgctaa acaatttttt accggtagca
    12301 tcagtaacct ccagccagca atcggcagta aagttcatca ccagcgcatt cggatcagcc
    12361 gccggcgtag tcacgcctgc ctgatcggtt ggcaacggcg cagcgccatc tggcgttgtt
    12421 gttgccgttg gtgccggggt cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc
    12481 gcattctgtt gcggatcaac agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg
    12541 gttgcggtag tatccacaga cgctggcggc gtggacgttg tagccgggtc tgtagttgtc
    12601 gacgtattta acggaacact ctgcccctgc tcgctattac tgctcagttc cgccgaagat
    12661 tgatcggcca tagtggtgat ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca
    12721 ccgctcaggc cgataaccac aaacaacacc agccaggtga aggtcatcag ccagccgtca
    12781 cgttttttgc ggcgtttacc gagggaaaaa ctctgcatag gcgcaacttt tgcagcccgt
    12841 agtggagcct gcttttccag ccctggcagc agttcttctt ctggaatatg taccagacgc
    12901 gcataagagc ggatatatcc gcgcaggaat gttgaagcaa gatcggcggg tgccttatct
    12961 tcttcaatgt cgcgtaccgt ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga
    13021 ctaagtccta gttgttcgcg agcattacgc aggcgagcgc cggtagtaag tgcttcattt
    13081 tggtcgtgcg tggcttcagt attcattcgc tgcaggtacg tttaaatgag aattgagatg
    13141 ccggtgaacc atcatgctca cccacaccgc gaaacatccg ttaagttaac cgttatcata
    13201 cagtataaga ccgtctgtgt gctcgtgaca aagcccacat tcactaaggc taacttactg
    13261 ttgcatcgtt acatactgcc ttaaagtcag caaaaacgca ccgttaatat tgaccagaca
    13321 attgcaactt aatacttcat tcataaaaga ttacgccacg gtacataaag taaccgtggc
    13381 gtaatggcta tcagaccgct ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg
    13441 tacgtttcgt acggtcgata acatcgcccg ccaactgacc acaggcagca tcgatatcat
    13501 caccacgggt tttacgcaca atagtggtga aaccgtagct catcagcact tttgagaaac
    13561 ggtcgatacg gctgttcgag ctgcgtccat acggcgcgcc cgggaagggg ttccacggga
    13621 tcaggttgat cttacacggc gtatctttca gcagttccgc cagttggtgc gcgtgttcag
    13681 tgccgtcgtt aacgtggtca agcatcacat actcaatagt gacacggccc tgattggcgt
    13741 tggatttctc cagataacgg cgcaccgcag caaggaacgt ttcgatattg tactttttgt
    13801 tgatcggcac aatttcgtca cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg
    13861 caacgtcgat catatcgccc agtttatcca gcgccggaac tacaccggaa gtagaaagcg
    13921 tgacgcgacg tttagacagg ccaaaaccga aatcatcaag catgatttcc atcgccggaa
    13981 cgacgttgtt caggttgagc agcggctcgc ccatgcccat catcactacg ttagtgattg
    14041 ggcgctgacc ggtgactttt gctgcgccga cgattttcgc cgcacgccac acctggccaa
    14101 taatttccga tacccgcagg ttgcggttaa agccctgctg ggcggtggaa cagaatttac
    14161 actccagcgc acaccccacc tgcgaagaga cgcagagcgt ggcacggtcg tcttccggga
    14221 tatacaccgt ttcgacgcgc tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg
    14281 atgaacgctg ttcttcaacc acttccggtg cgcggatttc cgccacctct ttcagtttgc
    14341 cgcgcaacac tttgttgatg tcggtcatct catcaaagtt gtcgcagcaa tagtgataca
    14401 tccacttcat cacctgatcg gcgcggaagg gtttttcacc taaatcttta aaaaactccc
    14461 gcatctgctg acggttgaga tccagcaggt tgatttttcc atctttcgtg gtgacgtttt
    14521 caggtgtgac taattgttca gacatatgct attccggcct cgttattaca cgttatggcc
    14581 cctggaggat tgaaaaaaga aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat
    14641 tgtacaaatt ctggcgcacg gatgccacgt ttgcacgcgg catttacgaa attattaacg
    14701 ggtgcgcggg cacacttcgc cttcgccaaa gaaataagcg atttcgcgag cggcagattc
    14761 gacggaatca gaaccgtggg taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag
    14821 agtaccagcc agtgcgtttg ccggattggt cgcgcccagc agatcgcggt gacgctgaac
    14881 ggcgttttca ccttccagca cggaaaccac gatcgggcca gaggtcatga attcaaccag
    14941 accatcaaag aacggttttc catcgtgttc agcataaaag ccacgtgcct gttcaacggt
    15001 caggtgcagc attttggtgc cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat
    15061 attaccaatg acgttttttg ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat
    15121 agccattttt acctctgtaa attgttctgt tgttgtctgt accagcgtac gagatggcgc
    15181 ggattataat gagcaacagg gccgttgact attgatgaag gtaacatttt tttaaaataa
    15241 aatgagtttt agcaacaatc aggggcaaaa cagactattg catgacaaat ttcactgtcg
    15301 cgatttgccc cacatcatcc atcaccagca attgataatc acctttatcc gtcaaatgca
    15361 gggtcacgtt gcgcccgcgt tcagttaacg gttcgccatt cagaaaccac cagcgttcac
    15421 ctgcccctcc acttgattgc aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa
    15481 taatcgcgcc atcgcgaacg cctgtcagct gcagagggag cttggcatcg tgaccgtatg
    15541 gcgggcaaat tgtcgaggcc ggtggtaagc gcacagcgcg acgttctgat gcgggcagcc
    15601 agggttccag cggtagcggc cagacattta tcatttcttg tcgcgcttgc gggcaatcag
    15661 cggcaacacg tttgccattt tcatccagcc agatggggaa acgaatgcca ttaatgcctt
    15721 cctgctccgg cagtaataga gttggcggct gactcccgtc cagcagccag gttgccaggc
    15781 ggcggcgaca gttaccgtca ccttccggca aagactgtcc gcccggccag cagataacgc
    15841 cacgagtgac tgagttcggg cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga
    15901 tattattgac ctgattcaac aatggtacgg cactggcaaa gccaaactga ccaacaacgg
    15961 gcgtgccgtc cggtctgcca gtccagatcc caatgacata gcgagcgtta accccaatcg
    16021 cccaggcgtc acgatagcca tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg
    16081 gcaaggcact atccggcaag ggttgcgctt catcagccat aatccggcga atgatccacg
    16141 ccgcccccga cgacattaaa ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt
    16201 tgcctgcctt gccgtggcga gcaaacgcgg tatacgctgc cgccatatct tccagttttg
    16261 caccagcgcc gccgagaatg agtgaaagat tcggcgcagc accgttgggc aaatataacg
    16321 gcaatccaac attgcgtaac tttgccgcaa accgtttcgg tccataggct tccagcacct
    16381 gcacagcagg taagttcagc gagcgcacca gcgcctcgct catgctgatc gggccatgaa
    16441 aaccgctatc aaagttacct ggtcgataat caccggtgcg ccgggggacg tcttgcagca
    16501 gtgatgccgg gtggatcaag ccttcatcca gcgccagacc ataaacaaac ggtttgagca
    16561 ccgatcctgg cgatcggatc gcattgacca tatcaacatg accaaagcgt gaatcatcgt
    16621 tgagatcaac cgatcccacc cagccgcgaa cacgcatatc ggtatgatca accacgatca
    16681 tcgccagtga gctgcgcggt ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac
    16741 gtcgttgaag accggcatcc agcgtagtaa cgattttgtc gcttttgctt ttaccgagca
    16801 tcatgcgcga aaacagcggt gccagttgcg gcatttgtcg gggtgccagc cagatgggtt
    16861 cttcccttga ctcttttacc tgctcacggg accacacacc ttgcacggcc atccgttcga
    16921 gcactttatt acgcgcggct tcggcacgct ccggccaacg atccgggcga agacggctgg
    16981 gcgcttgcgg caaaaccgcc agcattgccg cctcggaata gcttaaattc gcaggcgact
    17041 ttccgagata agcccaactt gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc
    17101 ggttaagata caaggtcaga atttcacgct tagacagatg ccattccagt tgcaacgcgc
    17161 gccagagctg gcgaattttg ccgccaaatg ttttggggtg aggatcaagc agacgagcaa
    17221 cctgcatagt gagcgtgctg ccaccggaaa taacccgtcc cgaagtgaga tcttgccatg
    17281 ctgcgcgcgc caccgagaat ggattcaccc ccggatgctt ccagaaccag cgatcttcat
    17341 aattgatcag cgcttcaagg taacgtggag aaacatcttc gattgttacc ggataacgcc
    17401 agatgccgtc agcatcggcg aagcgccata gcggcgtacc atcctgcgcc acgaccactc
    17461 gtgcgggatt gacttcatgc agcggtagag gccagagttt atctgctccc cacgctgcaa
    17521 gaaaaaggag aaatggcgca gctgccagcg ttatccagta gccgcgtttg gttaacaagc
    17581 gaggcattta cggtcggaca atcagcagat cttcagccgt gccggtcgcc cgccattggg
    17641 gaacatacat tgattccacc atcggttgcg gtacctgata cgttcccggc gtcaccgccc
    17701 gcgccagata caccagcgtt accggttggt attcatcaac ggcaaccgcc gccacaaagc
    17761 gatcgtcacg gaactcaatg tgcttaatac tcgcctgctg catctggttc agtaagtttt
    17821 gcacttcgcc accactttgc tccaggctgg cgctaccgtt cgccagattc tgattttcca
    17881 gttccagacc cgcaggcagc agatccacga ctaacgcatc cggcacgctg ttactggctt
    17941 ttacctgcaa ccagaccagc accagatcgc cgctacgtaa cgagtccagc gatttgctct
    18001 taccatcagt accaagaata tgacgctcga tttgcagcac attgttcgcc ggtaaaggtg
    18061 cggattgcgg ataaccgctg gcatccatac gcaaccataa cggctgatcg ccactgtttc
    18121 tcacctgcaa ggtggcaagt tgatcgctat tcagattgct gtttagcgtt ttctcgcctg
    18181 tcagcggctc agctgagaaa gaggtttgcg cctgccattt acccggtaaa tcctgaatcg
    18241 tacgggcagc caggaacaac gcgttacttt cctgcgtcga tagccagcgt tcaccaaacg
    18301 cctgctgcga aagtgtgttc agcaaagtgt actgctcatc gggtagtagt ttattttctt
    18361 ccagcaagga gagcattaac gcgttgtcgc gcagtggact accgtaatca cccagccata
    18421 tccgctcatc actatttctc ggcgttttca gcgccagcac aatcgcttct tcaccacgcg
    18481 ttgcatcacc catggttttc agcgcaacgc caagttgcag cagcggtaaa ccagaagcgg
    18541 catctgcacg atgctcccag atttcacgca gcgcacccag tggtgctttt tgctgacggg
    18601 ccagcaccag cgcagcgtaa gactgaacgg cgaatttact ggctttgaga ttatccgcgt
    18661 acgggatcga catcatgccc ggatcttgta aatagcgcag cagacgctca ttaccccggt
    18721 taatggcgtc tgtcggcacg ctgtaaccct gttcgcctgc gcggaccagg aaatccatca
    18781 cgtaagccgt cagccagtac tcttcgtcac cgtttttatc ccacagcgca aagccgccgt
    18841 tatcacgttg catttgcagc aaacgggaaa tgccgatatc gaccgatgca cggcgtttct
    18901 catcactgtc gcctttgatg cccaacgcct gcagttgggc tgcgttggta taaagtgacg
    18961 ggaacaggcc gctggcggtt tgctcaagac agccgtacgg atacgctttt aactctttga
    19021 tataacgtgc aatgttcagc ggtggtttgc cgctcaacaa caattgccct tccagcgtaa
    19081 caggcgagaa gttttgcaat ccatccgccg gaatcgccca tgtctcacca ggctgtaacg
    19141 ccgtaccgta attaaccgtt tgtgccggga acgccggacg gacgccgatt ttccactgct
    19201 tatgctgatc ggcaacggtt tcacccggta acgctaaccc gctaatggtg gcctgaattt
    19261 ctccatcgcc ataacccggc aatgctcgca ccgggataaa cagcgtagta cgcacacctg
    19321 gcgctaattc aacggctgcg ggtgaatcgc tgaccagttc aagcaaacca ctggcggtca
    19381 gggcaacgtt cagtttttgc ggtttatcgg taagattagt gatatccagc gtcagacgcg
    19441 aggtatcgcc actcgccata aagcgcggca tgttcagttc agcaatcacc ggtgcggcaa
    19501 ctatcacttt gctttcgttg ctaccgaaat catctgccgt ccaggcttgc gccatgacgc
    19561 gcaattcacc gttaaaatcg ccaatcggca gtgtaaccga gccttcgccc tgttcgttga
    19621 gcgttaccgg cagcgcctgc tgcgcgacaa tattgacgtg attgaccggc ggtttaccac
    19681 cacgtttcag ctcatcacca tcgccaccga aacgcagagc tgccagacgc ccctgacctt
    19741 caataacctg accgtaaata tcgtaaatgt ctgcgccata gcgtttctga ccaaagaacg
    19801 cctgccacgg atctggcgtg acgtagtcag taatattcag cacaccacta tcaacggcag
    19861 acaccagcac attcacctgt ttaggcttct cgccattttt agtgctggct ttaattttca
    19921 cggttaatgg ttgattggga cgcatttttg ctggtgtttc cagcgccaga tcgaggcgac
    19981 ggttttcatc gccaagcggc agatgcaaca caccaaccgc gcgttttggc gtcgcggagc
    20041 gagatttatc gccaggacgt accaccagcg tacttaaata cagatcatga cgattccagg
    20101 ttttatcgac cggaatcgtc agatccagcc cttgagcccg aacatcaatc tcttgccacc
    20161 acagcggccc ttcactggac tcgaccatcg cataaccttt acccgccgtt ggcgcggcga
    20221 tatgcaactt aatggtgtcg ccagggcgat aactggcttt atccagtttc agcgtgacac
    20281 ggtcgggtcg cactgcgccg ctaccgtcgc tgttgtcctg ccagctatag ccagcccaga
    20341 aacgaacact actgaccgct tcattcggcg ctttgacttc cagacgataa gcgccccact
    20401 ctaccggaaa actgaccttg ccggtttcgt ccgctttcag atccagagtt tgttcatttt
    20461 cgatcagatc tttttgatca aactgtgact gccagccttc atcttctgac cagttccagt
    20521 agtaatcgcg gcgttcgcga atcaggcgca cctgcaagcc cgacacggct tttttcacgc
    20581 cttgcgcatc gctataaacg atgtcaaaag cagcgttact gccttcatca acaatgggct
    20641 gtttcaccgt gctgtcagta cgataatcgt aaaccgattt cgaggcgaac tgcggacgga
    20701 tccccggcaa tgcatcggca ggccagatag cctgctcagc gcggcgcgtc accgggcgac
    20761 cgcccgattc cagcaaacta ccctggaaaa taacctgtaa tggggaatgc gtttccttcc
    20821 actggctttc tgtagaaact tcgccgcgcc ctttatcatc cagcgtcaac tgaacttcat
    20881 ccagcgtgcg ggaaagattt tcggcagcta tatcgccgaa ttcaaaacca ggtaaggctg
    20941 acacagcttc acgcagtggg cgcaggaaaa gttgcccttg caaagtatta ccattagcag
    21001 gtgcaccata caggtagtac cccaccacgg agaatttcac ttcatctttc ggcgttagcg
    21061 gggttttctc accggtcaga ttcagcgcca tgcgctctgg cataaaatct tcgacgtgga
    21121 aatcccacat ccgatactgg ttatcgcccg tgttagcgcg aatatgccac ataccggttg
    21181 ccgcattgct atcgagtggc caggtaaagt ggtagaggcc attctccggc tgactaacga
    21241 cgctcctgag tacctgccca tcgggtttaa tcacgtctaa cttaatgggt tgattgggca
    21301 acgctttacc gtctgcatca cgcagcaaac cattgaggat taccgtttca cctgggcgat
    21361 aaagatcgcg tgggccaaac atgaaaaact gtttgctata gcctggcgcg ccagcaatgt
    21421 taaattctgc taagtccagc gccggaagtt ttaaatcgag tagcgttgtc tgaccgtctt
    21481 tacgcgccaa cagtaatgcc gcgtttttat cattttccag ctgcacatgc ccctgagcgt
    21541 cactggttgc ctgagtcaga gtctgccctt tctcatttaa taaagagact tcaattcctt
    21601 gctgggccgc gccgttttcc agactttggg taaagatatc cagacgattg tgataacggt
    21661 gagctgaaac gccgatatca cttaacgtaa acagcgtcgc gggattactg taatcgtaac
    21721 gtccagcctg attcatcaca gccagataca cgcccgcctg ctgaagcggt ttgatatcgc
    21781 ccagcggcag caataatttt tcacgggtgt tacgcgcagg attgagatca aaccgtccgg
    21841 tgtagaccag atccgccatc tgcagcagtt tgtctgactg ccagttcgcc agcgaattgc
    21901 ggtattccca ttggctaatg aatgctggca gagattctgg cttaacgcgg aagaagttaa
    21961 catcgacatt attaacgttg agcgccatta ccggcagccc ttcaacgact ttgccaggca
    22021 gcagcgaacc acggctggca aaaccgacgc tgggttggat gtcgcgggta gttatagttt
    22081 tttcgtaatc tttactgaag gttgcgttgt tgagtgcttt gacctccttg ccaatagtaa
    22141 cgatcaaatc acgtttcggt tcgaggtggc gtaaacgtaa ctctttaaga ttatctgaca
    22201 gctcccaggc accatccact ttgccgcttt ttttatcgac gacatgaata acgcgtgaga
    22261 aatcctgatc cgggtcgaga gggatggaga acgtcagcac cagcgtggct gcaccatcaa
    22321 gttggacttc agagagatcc agcaatgtca gcaccttacc ggcactctgt tgggccagtt
    22381 tttgtctctc cggcacggag agttttgctg aactcgcgtt ttctgaagag gccgctttag
    22441 taacttcaga aggcgcatct tttttcaccg ctgttggcgc gttatcgttg ttgtcgcacc
    22501 ctgccagcgc cagcattagc atgcaggcgg ctacgcgtaa ctttttcatt tttcatccct
    22561 gccacaatgg cccgttagca acgtcgaata attattatgc gtgagaattt acgttatgta
    22621 attcagtatc acgcccatac aatccgaatt ttagtgagaa ttgtggctca gacagccaaa
    22681 tcagcctctt tcgcttgtcg cttttcacta aacatccgac aatttagcct aaccccggca
    22741 aaaatggaga tgcctatgtc cacgacatgg tttgtaggag ccgactggct cgccgaacat
    22801 attgatgacc cggaaattca gattattgat gcccgcatgg cgtcgcctgg acaggaagat
    22861 cgtaacgttg ctcaggagta tctgaatgga catattcccg gcgcagtgtt ttttgatatc
    22921 gaagcgcttt ctgatcacac ttccccgctt ccgcacatgc tgccgcgccc ggaaacgttc
    22981 gccgtggcga tgcgtgaatt aggcgttaac caggataagc acctgattgt ctatgacgaa
    23041 ggtaatcttt tctcagcccc acgagcatgg tggatgctgc gcacctttgg tgtagagaaa
    23101 gtgtcgattc tggggggtgg acttgcaggc tggcagcgcg atgatctgct gttagaagaa
    23161 ggtgcagtag agctgccgga aggagagttt aacgccgcgt ttaatcctga agccgtggtg
    23221 aaagtaaccg atgtattatt ggcaagccat gaaaatacgg cgcaaattat tgatgcccgc
    23281 ccggctgcac gttttaacgc agaagttgat gaacctcgcc caggtttacg tcgcggacat
    23341 attcccggag cactgaatgt tccgtggacg gaactggtgc gcgaaggcga actaaaaacg
    23401 accgatgaac tggatgcgat attttttggt cgcggcgtca gctacgacaa accaattatc
    23461 gtcagctgcg gctctggtgt aacggcagcc gtggttttgt tagcactcgc gacgctggat
    23521 gtgccaaacg tgaaactgta cgacggcgca tggagtgaat ggggcgcgcg ggcagattta
    23581 ccggttgagc cagtgaaata agtattttac aggcaataaa aaaccgccga atttggcggt
    23641 tttttattgc tggtctggtt cgcggccttt ccagcaggtt gtattaccgt agtaatgcaa
    23701 gcgcgtctca gcggagacaa tactcgccag taactctctt tttgtcaagc aaaagagagt
    23761 tattattgtt ctgttagtgt attatccact gcggcccttt ccgccgtctc gcaagcgggc
    23821 gctggcttaa ggaaaggatg ttccgcgacc gttagagcaa gcgcgtctca gcgcttgcta
    23881 tcgcggcaat atcgccagtg gtgctgtcgt gatgcggtct tcgcatggac cgcacaatga
    23941 agatacggtg cttctgtatc gtacttattg tttctggtgc gctgttttcc caagtaaata
    24001 ataaccggag tctctccggc gacaatttac tggtgg
//
LOCUS       DAZFVO020000068        24032 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFVO020000068 DAZFVO020000000
VERSION     DAZFVO020000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 24032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.068"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            181..684
                     /gene="ftnB"
                     /locus_tag="ACHKNB_RS12525"
                     /old_locus_tag="pgaptmp_002505"
     CDS             181..684
                     /gene="ftnB"
                     /locus_tag="ACHKNB_RS12525"
                     /old_locus_tag="pgaptmp_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HGU4196000.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(763..1014)
                     /gene="yecJ"
                     /locus_tag="ACHKNB_RS12530"
                     /old_locus_tag="pgaptmp_002506"
     CDS             complement(763..1014)
                     /gene="yecJ"
                     /locus_tag="ACHKNB_RS12530"
                     /old_locus_tag="pgaptmp_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HGU4196001.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1129..1215)
                     /gene="azuC"
                     /locus_tag="ACHKNB_RS12535"
                     /old_locus_tag="pgaptmp_002507"
     CDS             complement(1129..1215)
                     /gene="azuC"
                     /locus_tag="ACHKNB_RS12535"
                     /old_locus_tag="pgaptmp_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HGU4196002.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1478..1801
                     /gene="yecR"
                     /locus_tag="ACHKNB_RS12540"
                     /old_locus_tag="pgaptmp_002508"
     CDS             1478..1801
                     /gene="yecR"
                     /locus_tag="ACHKNB_RS12540"
                     /old_locus_tag="pgaptmp_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HGU4196003.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1972..2469
                     /gene="ftnA"
                     /locus_tag="ACHKNB_RS12545"
                     /old_locus_tag="pgaptmp_002509"
     CDS             1972..2469
                     /gene="ftnA"
                     /locus_tag="ACHKNB_RS12545"
                     /old_locus_tag="pgaptmp_002509"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HGU4196004.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2507..2746)
                     /gene="yecH"
                     /locus_tag="ACHKNB_RS12550"
                     /old_locus_tag="pgaptmp_002510"
     CDS             complement(2507..2746)
                     /gene="yecH"
                     /locus_tag="ACHKNB_RS12550"
                     /old_locus_tag="pgaptmp_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HGU4196005.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            2937..4148
                     /gene="tyrP"
                     /locus_tag="ACHKNB_RS12555"
                     /old_locus_tag="pgaptmp_002511"
     CDS             2937..4148
                     /gene="tyrP"
                     /locus_tag="ACHKNB_RS12555"
                     /old_locus_tag="pgaptmp_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HGU4196006.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4210..4875)
                     /gene="yecA"
                     /locus_tag="ACHKNB_RS12560"
                     /old_locus_tag="pgaptmp_002512"
     CDS             complement(4210..4875)
                     /gene="yecA"
                     /locus_tag="ACHKNB_RS12560"
                     /old_locus_tag="pgaptmp_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HGU4196007.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKLGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5071..5157)
                     /locus_tag="ACHKNB_RS12565"
                     /old_locus_tag="pgaptmp_002513"
     tRNA            complement(5071..5157)
                     /locus_tag="ACHKNB_RS12565"
                     /old_locus_tag="pgaptmp_002513"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5121..5123),aa:Leu,seq:taa)
     gene            complement(5170..5243)
                     /locus_tag="ACHKNB_RS12570"
                     /old_locus_tag="pgaptmp_002514"
     tRNA            complement(5170..5243)
                     /locus_tag="ACHKNB_RS12570"
                     /old_locus_tag="pgaptmp_002514"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5209..5211),aa:Cys,seq:gca)
     gene            complement(5297..5372)
                     /locus_tag="ACHKNB_RS12575"
                     /old_locus_tag="pgaptmp_002515"
     tRNA            complement(5297..5372)
                     /locus_tag="ACHKNB_RS12575"
                     /old_locus_tag="pgaptmp_002515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5337..5339),aa:Gly,seq:gcc)
     gene            complement(5524..6072)
                     /gene="pgsA"
                     /locus_tag="ACHKNB_RS12580"
                     /old_locus_tag="pgaptmp_002516"
     CDS             complement(5524..6072)
                     /gene="pgsA"
                     /locus_tag="ACHKNB_RS12580"
                     /old_locus_tag="pgaptmp_002516"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGU4196008.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(6129..7961)
                     /gene="uvrC"
                     /locus_tag="ACHKNB_RS12585"
                     /old_locus_tag="pgaptmp_002517"
     CDS             complement(6129..7961)
                     /gene="uvrC"
                     /locus_tag="ACHKNB_RS12585"
                     /old_locus_tag="pgaptmp_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGU4196009.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7958..8614)
                     /gene="uvrY"
                     /locus_tag="ACHKNB_RS12590"
                     /old_locus_tag="pgaptmp_002518"
     CDS             complement(7958..8614)
                     /gene="uvrY"
                     /locus_tag="ACHKNB_RS12590"
                     /old_locus_tag="pgaptmp_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HGU4196010.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8756..8827
                     /locus_tag="ACHKNB_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /pseudo
     CDS             8756..8827
                     /locus_tag="ACHKNB_RS12595"
                     /old_locus_tag="pgaptmp_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8910..9086
                     /gene="yecU"
                     /locus_tag="ACHKNB_RS12600"
                     /old_locus_tag="pgaptmp_002520"
     CDS             8910..9086
                     /gene="yecU"
                     /locus_tag="ACHKNB_RS12600"
                     /old_locus_tag="pgaptmp_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HGU4196011.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9073..9297
                     /gene="yecF"
                     /locus_tag="ACHKNB_RS12605"
                     /old_locus_tag="pgaptmp_002521"
     CDS             9073..9297
                     /gene="yecF"
                     /locus_tag="ACHKNB_RS12605"
                     /old_locus_tag="pgaptmp_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HGU4196012.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9364..10086)
                     /gene="sdiA"
                     /locus_tag="ACHKNB_RS12610"
                     /old_locus_tag="pgaptmp_002522"
     CDS             complement(9364..10086)
                     /gene="sdiA"
                     /locus_tag="ACHKNB_RS12610"
                     /old_locus_tag="pgaptmp_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HGU4196013.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10316..11068)
                     /gene="tcyN"
                     /locus_tag="ACHKNB_RS12615"
                     /old_locus_tag="pgaptmp_002523"
     CDS             complement(10316..11068)
                     /gene="tcyN"
                     /locus_tag="ACHKNB_RS12615"
                     /old_locus_tag="pgaptmp_002523"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HGU4196014.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11065..11733)
                     /gene="tcyL"
                     /locus_tag="ACHKNB_RS12620"
                     /old_locus_tag="pgaptmp_002524"
     CDS             complement(11065..11733)
                     /gene="tcyL"
                     /locus_tag="ACHKNB_RS12620"
                     /old_locus_tag="pgaptmp_002524"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HGU4196015.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11748..12734)
                     /gene="dcyD"
                     /locus_tag="ACHKNB_RS12625"
                     /old_locus_tag="pgaptmp_002525"
     CDS             complement(11748..12734)
                     /gene="dcyD"
                     /locus_tag="ACHKNB_RS12625"
                     /old_locus_tag="pgaptmp_002525"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HGU4196016.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12839..13639)
                     /gene="tcyJ"
                     /locus_tag="ACHKNB_RS12630"
                     /old_locus_tag="pgaptmp_002526"
     CDS             complement(12839..13639)
                     /gene="tcyJ"
                     /locus_tag="ACHKNB_RS12630"
                     /old_locus_tag="pgaptmp_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4196017.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13727..14278)
                     /gene="fliZ"
                     /locus_tag="ACHKNB_RS12635"
                     /old_locus_tag="pgaptmp_002527"
     CDS             complement(13727..14278)
                     /gene="fliZ"
                     /locus_tag="ACHKNB_RS12635"
                     /old_locus_tag="pgaptmp_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HGU4196018.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14324..15043)
                     /gene="fliA"
                     /locus_tag="ACHKNB_RS12640"
                     /old_locus_tag="pgaptmp_002528"
     CDS             complement(14324..15043)
                     /gene="fliA"
                     /locus_tag="ACHKNB_RS12640"
                     /old_locus_tag="pgaptmp_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HGU4196019.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15364..17016)
                     /gene="fliC"
                     /locus_tag="ACHKNB_RS12645"
                     /old_locus_tag="pgaptmp_002529"
     CDS             complement(15364..17016)
                     /gene="fliC"
                     /locus_tag="ACHKNB_RS12645"
                     /old_locus_tag="pgaptmp_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HGU4196020.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITSSNGDKLYIDTTGRLTKNGFSASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPNTITYSVTGAKVDQAAFDKAVSTSGNDVDFTTAGY
                     SVDGATGAVTKGVAPVYIDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            17282..18688
                     /gene="fliD"
                     /locus_tag="ACHKNB_RS12650"
                     /old_locus_tag="pgaptmp_002530"
     CDS             17282..18688
                     /gene="fliD"
                     /locus_tag="ACHKNB_RS12650"
                     /old_locus_tag="pgaptmp_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HGU4196021.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTIS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            18713..19123
                     /gene="fliS"
                     /locus_tag="ACHKNB_RS12655"
                     /old_locus_tag="pgaptmp_002531"
     CDS             18713..19123
                     /gene="fliS"
                     /locus_tag="ACHKNB_RS12655"
                     /old_locus_tag="pgaptmp_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HGU4196022.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            19123..19488
                     /gene="fliT"
                     /locus_tag="ACHKNB_RS12660"
                     /old_locus_tag="pgaptmp_002532"
     CDS             19123..19488
                     /gene="fliT"
                     /locus_tag="ACHKNB_RS12660"
                     /old_locus_tag="pgaptmp_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HGU4196023.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            19566..21053
                     /gene="amyA"
                     /locus_tag="ACHKNB_RS12665"
                     /old_locus_tag="pgaptmp_002533"
     CDS             19566..21053
                     /gene="amyA"
                     /locus_tag="ACHKNB_RS12665"
                     /old_locus_tag="pgaptmp_002533"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGU4196024.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(21087..21500)
                     /gene="yedD"
                     /locus_tag="ACHKNB_RS12670"
                     /old_locus_tag="pgaptmp_002534"
     CDS             complement(21087..21500)
                     /gene="yedD"
                     /locus_tag="ACHKNB_RS12670"
                     /old_locus_tag="pgaptmp_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HGU4196025.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21687..22892
                     /gene="yedE"
                     /locus_tag="ACHKNB_RS12675"
                     /old_locus_tag="pgaptmp_002535"
     CDS             21687..22892
                     /gene="yedE"
                     /locus_tag="ACHKNB_RS12675"
                     /old_locus_tag="pgaptmp_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HGU4196026.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22889..23122
                     /gene="yedF"
                     /locus_tag="ACHKNB_RS12680"
                     /old_locus_tag="pgaptmp_002536"
     CDS             22889..23122
                     /gene="yedF"
                     /locus_tag="ACHKNB_RS12680"
                     /old_locus_tag="pgaptmp_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HGU4196027.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23231..23902
                     /gene="yedK"
                     /locus_tag="ACHKNB_RS12685"
                     /old_locus_tag="pgaptmp_002537"
     CDS             23231..23902
                     /gene="yedK"
                     /locus_tag="ACHKNB_RS12685"
                     /old_locus_tag="pgaptmp_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HGU4196028.1"
                     /translation="MCGRFAQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(23942..>24032)
                     /locus_tag="ACHKNB_RS12690"
                     /old_locus_tag="pgaptmp_002538"
     CDS             complement(23942..>24032)
                     /locus_tag="ACHKNB_RS12690"
                     /old_locus_tag="pgaptmp_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196029.1"
                     /translation="GHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         5933 a         6150 c         5800 g         6149 t
ORIGIN      
        1 acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt tcagatcatc
       61 gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag
      121 taattatggt gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt
      181 atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca
      241 tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc
      301 actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt
      361 atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg tgaaaaactg
      421 aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg ttctagtacg
      481 ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg
      541 cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat tcttgatgaa
      601 gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat
      661 gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc cgatgaaggt
      721 tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg
      781 tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca tggtgaccgg
      841 gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg acattttttc
      901 acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg
      961 gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa
     1021 ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac
     1081 atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt atcggaacat
     1141 attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat
     1201 tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat tgtctttgcg
     1261 cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc acataaatac
     1321 ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag
     1381 atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc actgtaacgc
     1441 gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct
     1501 ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga gcgaagttga
     1561 cgcggcaaca ggtattgtac ggttagttta tgaccaggct tttttgcagc atgcccatac
     1621 cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca
     1681 cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt
     1741 gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta
     1801 aatattttaa tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat
     1861 tcccattcgt atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa
     1921 caaattatag tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa
     1981 ccagaaatga ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt
     2041 tatcagcaaa tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg
     2101 cgccgtcacg cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat
     2161 accggcaatt taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt
     2221 gatgaattat tccaggaaac ctataaacac gaacaattaa tcacccagaa aattaacgaa
     2281 ctggctcatg ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat
     2341 gtttctgagc agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg
     2401 gcaggcaaaa gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca
     2461 caaaactaat gctctccggc ggcaggttta ctgccgccgt ttcaaatcag tgacggcaaa
     2521 tcttactctg atcggtcgaa aaaccgtctt tcgaaggtat aaatttgcct tttgctgcca
     2581 gaaacgctac cagctctccc gctgtcatcc cttctgccga gcaggtgtga aaacgtgcct
     2641 gttcaccaaa acgcgcttta atcgcagctt ccagactggc atgcgtatat tgctcgcctg
     2701 attcaatcat catatttaac acttcatgac cgtgaataga gtccatcgtc cctcctcaaa
     2761 aaaagcctag cgtagcgatt gccgcttatg aagactttgc gccagcgcag gactgaatgc
     2821 tttttattgt acatttatat ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta
     2881 tgccttaacc tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga
     2941 aaaacagaac cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc
     3001 tggcaatgcc gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg
     3061 ggctttgggc gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc
     3121 cggcagatac cggtctgggc acgctggcaa aacgctatct gggacgctac ggtcaatggc
     3181 tgacgggctt cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg
     3241 ccggtgaatt gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg
     3301 ctggcgtgct gttgttcact tttgttgccg gtggcgtggt ttgtgtcgga acttcactgg
     3361 tcgatttatt taaccgtttt ctgttcagcg ccaagattat ttttctggtg gtaatgctgg
     3421 tactactgct gccgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc
     3481 tggctctgtc tgcaatcccg gtgattttta cctcatttgg ttttcacggt agcgtgccga
     3541 gtattgtcag ctatatggat ggcaacgttc gtaagctacg ctgggtgttt ataaccggta
     3601 gtgcgatccc cctggtggca tatattttct ggcaggtggc aacgcttggc agcattgatt
     3661 caacaacctt tatgggattg ctggctaatc atgccggatt aaacgggctg ttacaggcat
     3721 tacgcgaaat ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag
     3781 ccctcgccac gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttat
     3841 ttcagcgttc aaataccgtt ggtggacggt tgcaaactgg tgcaattacc tttctgccgc
     3901 cgttggcgtt tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg
     3961 tggcgctggc ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcagaaagc
     4021 acaatcctca ggcgggttac cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc
     4081 tctgtggtat tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag
     4141 tggggtgatc agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa
     4201 tttgacctct taatgcaggc agcactgctt aaatttctta ccactaccgc acgggcaagg
     4261 atcgttacgc cccagtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg
     4321 atgcgccatc cagtacgcat gtagatcaag cgccgccagt cgaatggcat ctacgctctc
     4381 ttcaaacgct tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag
     4441 cgcaatcgcc tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc
     4501 cacgccccgc atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac
     4561 ttctcgcaag ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt
     4621 atcggccata tgttgaaaag ccagattcat aaagcgcgtc atctctttct ctgacgccca
     4681 gcgcggcaca tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc
     4741 ttgcggagaa ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc
     4801 gtggtcagtg ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa
     4861 cggtcccgtt ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat
     4921 gccggtccgt atcagagata ctttttgagt ggctttgctg gtgattaaaa attaaggagg
     4981 gtgtaacgac aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt
     5041 attgctttga ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac
     5101 agcgcgaacg ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg
     5161 ggaagaaagt ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta
     5221 cataaccgct ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgctgacg
     5281 ccgatgtctt ttaaactgga gcgggaaacg agactcgaac tcgcgacccc gaccttggca
     5341 aggtcgtgct ctaccaactg agctattccc gcattcatca agcaatcagt taatcacttg
     5401 attttattat cgtctggcaa tcagtgccgc cgttcgatgc gttgcattct acttacctgg
     5461 cgcgatgagt caacgatatt tttcaccact tttgattgtt tgctgaaaat tacgccgaaa
     5521 cgatcactgt tcaagcaaat ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag
     5581 agtcagtacc gcagccacaa agaaaagtgc aataccggcg tactcaaccc aaatgttcgg
     5641 acgccacagc agccaggcca acgccaccat ctgggcagtg gttttcactt tcccaatcca
     5701 ggagacagcc acgctactgc gtttacccaa ctccgccatc cattcgcgta gcgcagaaat
     5761 aataatttca cgggcgatca tcgttgccgc cggtaaggtc acccaccagc tgtgataatg
     5821 ctcggttacc agcaccatgg cgatagccac gagaacttta tctgcgacag ggtcaaggaa
     5881 agcaccaaac cgggtactct ggttccagcg gcgtgccaga aaaccatcga accagtcagt
     5941 caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc gaccaggtga caggcagata
     6001 aaagaccaat acaaagaatg ggataaggat gacacggaac agtgtaagca acgtagggat
     6061 attaaattgc ataatgacgg gtaactatct gttgtcagta agattacccc tatgttgcta
     6121 cagagacatc aatgtttcaa cgaccagaag atcttttctg ccagaccttg cgaaataccc
     6181 ggcacttttg caatttcctc gacgctggcg ttacgtaaac cttgcaaacc gcccatatat
     6241 ttcaacaaca tttgccggcg ttttggcccg acgccttcaa tggtttccag ggaactggta
     6301 tttttgacct tcgcccgttt tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg
     6361 cgaatatgct ggataacatg cagcgcggga gaatctggcg gcaaactgaa tccctcacct
     6421 tccggctcaa agaacagcgt ttccagccca gccttacgat ctgctccttt ggcaacgcca
     6481 agtagcagcg gatgattttt atcccatgag acatccagtt cggcgaagac atttttcgcc
     6541 tgcgcaagct ggcctttgcc gccgtcgata agtatcacat ccgggatctt actgtcgtcg
     6601 atggctttac cataacgccg acgcagcacc tgattcatcg ccgcataatc atcgcccggc
     6661 gtgatgccag taatgttata gcgccgatac tccgcacgca gcgggccgtt agcatcaaac
     6721 accacacagg aagcgacggt ttgttcgccc atggtatggc tgatgtcaaa gcactccatc
     6781 cgcttcactt ccggcaattt caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta
     6841 gattgctgcg aaagtttgct ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga
     6901 taacgcgccc tatcgccgcg aggtttggtt tgaacattaa tcttgcgtcc cgccagttct
     6961 gaaagggaat cggcgagcag cgttttatcg ctaagattaa aatcgagcag gatctcaccc
     7021 ggtaaggtgc gcatctggct gccttgtaaa tagaactggc ctacgaaggt ttctaccacc
     7081 tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc tgcggctgcc gagcactttg
     7141 ccctgacgaa tgaacaatac gtggacacaa gccatgcccg catcgaacgc cacaccaata
     7201 acgtcgaggt cgtcgccggt attggaaaca aactgttttt cggtgacgcg tcgcaccgcc
     7261 tgaatttggt cgcgaatacg tgcagcttct tcaaactcca gattctggct ggcagtttcc
     7321 atacgactaa tgagttgcgt aagcacctga tcatctttgc cagacaaaaa caggcgcaca
     7381 tactcgacct gctgagcgta ttcttcttca ctcaccagtc cttcaacgca cggtcccaga
     7441 cagcgcccta tctggtattg cagacacgga cgcgagcgat tgcgataaac actattttcg
     7501 cactggcgaa tggggaaaat cttttgtagt agcgccagtg tttcacgtac ggcatagcca
     7561 ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg caccacgatg catcgccaga
     7621 cgcgggtggg tatcaccact caggaagata aaaggatatg atttatcatc gcgtagcaaa
     7681 acgttgtaac gcggctgata gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt
     7741 tctgtgtgag taaccgttac atcaatttgc tggatctggg cgaccagcgc ttcggttttg
     7801 cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg
     7861 ccgacataga taaccgtacc accagcatcg tacatgcgat aaacgcctgg ctggctggtt
     7921 acggttttta aaaacgcttt tgcgtcaaac tgatcactca ctgacttgat aatgtctccg
     7981 cattacacag accatggcga attgccaggt gagtcagctc aacatcgcca tgaatgttta
     8041 gtttactgaa catacgatag cggtagctgt tcaccgtttt cggactgaga ttgagctgtt
     8101 ctgagatctc attgaccttc tggcccttgg tgatcatcag cataatctgc aattcacgtt
     8161 cagacaaact ggcaaatggg ctttctgttt tttctggttc gatctggctt aacgccattt
     8221 gttgagcgat gtcagaagca atgtaacgct gccctgaata gacagaacga atcgcactca
     8281 cgacttcctg cggagccgcg cctttgctga ggtagcccgc agcaccggcc tgcatgactt
     8341 tcgctggtaa agggttttct gtatggacgg taagcatgat gattttgaca tcagctgtgg
     8401 aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc cggcatactc atgtccatta
     8461 gcaccacgtc aacggcattt gtccggcacc acttaacggc gtcttcaccg cacgatgcct
     8521 caccgacgac ttttataccc tttatatctt ccagaatgcg tcgtatccct gcgcgcacca
     8581 gttcgtggtc atcaacaagt agaacgttga tcaaaggaat atctccagaa atagggataa
     8641 cgctactgat agttagtcat tcgtatatta gcggttttta ttgcaacttt gaaacgttaa
     8701 aaatggtggg tattcgattt ttctctcgtt tttgtcaatt cgattgtcca cagttatggt
     8761 cacgcccgtc accaacggct taccggatcg tttcacagta agccctacga tgaaatgtga
     8821 caaaaattga ctttattcag caaaaatgaa aatcagcccg ctgaatatgt aacattaatt
     8881 aacccgaaca atacgggcaa aaaacattca tgataaaaat atttattggt cattatatta
     8941 acgtatttta tagcactgcc gatatcacgc tcaaaaaaca accactgcta tttttagcaa
     9001 agcttatggt atactccgcc gccttaacat ttttcaccgc aaattttcat tgcaacatga
     9061 cgaggaaaat aaatgagtac gcctgatttt tctactgccg agaataatca agaactggca
     9121 aatgaagtct cctgcctgaa agcgatgctg acgctgatgc tgcaggcgat gggacaagct
     9181 gacgcgggcc gcgtgatgtt aaagatggaa aaacagcttg cgctgatcga agacgaaacc
     9241 caggctgcag tattttccaa aacggttaag caaattaaac aagcctaccg tcagtaatat
     9301 aaaaccggct gagagcgtgc ctttcagccg gtttttgcat ctggcacgca gaaagaaaag
     9361 cgatcaaatt aagccagtag cggccgcgta acaggccacc tgggtcttat ttggtgcatt
     9421 gatttttttc tgcatgtttt tctgatggaa attgaccgta ttctcagaga ttgacaaaat
     9481 catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcagaattt ctttttcgcg
     9541 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
     9601 cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
     9661 ttcgcgcgtg ctgcaacggg aaaaggacaa aaagcccagc gcccgatttg gcagcattaa
     9721 atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc
     9781 ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag
     9841 caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg cctcagggta
     9901 attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga
     9961 atagtaatcg tactccagct gctgagcctg aagctctatt tcatggtaga cctcttctgc
    10021 ggcctccatc ctctgaaaac gcaacagcat cgtgcgacgc cagctgaaaa aatccgtatc
    10081 ctgcatagta aaccgcaacg cccctgagag tgagtatcat ttataatgat aattcaaact
    10141 atcacataaa taagatttat atataattta tattattcag gcaatgaatt acttttgcaa
    10201 gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca gttttatcac
    10261 gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt tcttattatt
    10321 gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatca gcaaataacg
    10381 cttttgctgc cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
    10441 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
    10501 gctggcggat tgtgttcagg acttcgccca ccagctctgg atccagcgct gacgttggct
    10561 cgtcaaacaa aatcacctca ggacgcattg ccagcgcacg cgcaatcgca acacgctgct
    10621 gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc ccaactttcg
    10681 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
    10741 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag ttctggaaga
    10801 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
    10861 cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt tccagcagat
    10921 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt gccaccactt
    10981 cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga aattttttca
    11041 ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat taagttgatt
    11101 ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga tgagcgaagc
    11161 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cttgacggaa
    11221 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc tgataaagct
    11281 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
    11341 ctgccacggc gtcataccaa tactggcagc ggcttcccac tgacctttat caatagaaga
    11401 aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcagcgaca gaccaatcat
    11461 tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat agatcataaa
    11521 cagttgtgcg atgagcggtg taccacgaaa aatagagata taaaaacgcg ccagccagcg
    11581 cacgggccag attggcgaca gacgcatcag cgcgagaata aaccctagca gtaagccaaa
    11641 aaacatgccg ccgatactga gttgcagggt atacccagcc cctttcaaca ggaacggcaa
    11701 ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta aacgtgggga
    11761 tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg cccttcatct
    11821 ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc ggtatacaca
    11881 ggatcaagca gaatgccttc cagacgcgcc agcaatttca ctgcttccat gccttcgtcg
    11941 ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc cgctgatgcg
    12001 gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg caattgatcg
    12061 gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag gtgttccagc
    12121 ccaacagcca atccggcgtg agttccggca ctgcccgatg cgaccaccac cgacgaaata
    12181 ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata acccagcgcg
    12241 cccagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg tgcttcgact
    12301 cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca catttcaatc
    12361 tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt ttctgcggtt
    12421 gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc cgcgactgcg
    12481 gcagtctggc gcacatggtt agactggatc gcgccggcag taatcagcgt atcggcacct
    12541 tcgcgcaggg catctgccgc gagaaattcc agcttacgta atttattgcc gcccattgcc
    12601 atgggtgtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc agaaaagcgc
    12661 ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa acgggttaaa
    12721 ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat tatgcacgct
    12781 gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat tatgctgatt
    12841 atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg ccgtcttttt
    12901 gcatttccgc aattgcatca ttcaccgctt tcagcaggtc ttcatttcct ttacgcagcg
    12961 ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta tcgttggttt
    13021 tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata cggcctacgc
    13081 gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg ccctgaacat
    13141 tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt ttgcctttca
    13201 gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc tgaataccgg
    13261 aaatggtgta aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa atggtgacct
    13321 gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg tcccatttgg
    13381 tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca aattccactt
    13441 caaaaccggt taatttgccg tcatctccct gaaaactgaa cggcggataa gtgccttcca
    13501 gccctaccag cagcgtgccg cgctctttaa ctttattaag cagaccttca tctgcaaaac
    13561 ttttaacgct catgcccgca accagcgcta cggccatcac acccatcaat gcctgacgtc
    13621 ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc agtgtagagc
    13681 cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa tatatatcag
    13741 aagcaggcag gctggaggat ttctgcacaa gtccggtaca cgtttgacgc tgatagtgtt
    13801 gatacttccg taatgcgatg cggtaattgt tggtgctggt ggtgggcagc cacggggcga
    13861 ggatttcatc aaggaaaccg tcctgcagcg gatcgaggga aatattttgc tcgtgtagat
    13921 gattccccaa acggcgcaga cgaacgacat attcacgcac cgtaccaggg ctcatttcag
    13981 tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc tgcgttttgc
    14041 aatgtaaatc accgcagaat cggcacaatg ccgcccatga tttttgttcc acttgccagc
    14101 cattttcatc aagcagcatg tccaggcggg aaatctcgat tttattcact attttgccgt
    14161 cgcgaacgag ggtaatgcgg tcgagtaatt tacggcaatg cgcgcaatgg gtctggctgt
    14221 gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc accatcatta
    14281 agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac ttacccagtt
    14341 tagtgcgtaa ccgtttaatg gcctggctgt gtaactgact gacccgcgat tccccgacct
    14401 ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg gttaatacca
    14461 gtttttcgcg ctccggcaac gtttcgatag cttccatcac tcgctggcgc agattactgt
    14521 ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt tcgatgctat
    14581 cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg gtgtcgagca
    14641 acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca gtttccgtgg
    14701 cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca cgcgcgttgc
    14761 gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata gcgccacgga
    14821 tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag cgttcgacgg
    14881 cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg ctcgcgggca
    14941 gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc tgccacagcg
    15001 agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac agccctgcgt
    15061 tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga cgattagtgg
    15121 gtgaaatgag gggttatttg ggggttaccg gtaaattgcg gacagaaaaa aaccccgccg
    15181 gtggcgggga agcacgttgc tgacaaatta ccattcatgt tgccggatgc ggcgtaaacg
    15241 ccttatccgg cctacaaaaa tgtgcaaatt caataaattg cagttcacct tgtaggcctg
    15301 ataagcgcag cgcatcaggc aatttggcgt tgccgtcagt ctcagttaat caggttacga
    15361 cgattaaccc tgcagcagag acagaacctg ctgcggtacc tggttagctt ttgccagcac
    15421 ggagttaccg gcctgctgga tgatctgcgc tttcgacatg ttggacactt cggtcgcata
    15481 gtcggcgtcc tgaatacggg actgcgcttc ggacaggttg gtagtggtgt tgttcaggtt
    15541 ggtgaccgcg gaatccagac ggttttgcac cgcaccgagg gaggagcgga atttgtcgat
    15601 ggagctgatg gcttcgtcca gagctttcag tggatcggcg gtggttgcag ctttagtttc
    15661 agcatctgtc gtcaatttac cgtcagcatc tttataaact gccttaccgc tgccgttagt
    15721 cactgaacca tcatcctgta gataaaaatc tacagtatca gatgtggtca acgccccgtt
    15781 gttatcaata taaaccggag caacaccttt tgttacagcg ccagttgcgc cgtcgacgct
    15841 ataacctgcg gtagtgaaat caacatcgtt tccagaggtt gatacagctt tatcgaaagc
    15901 tgcctgatca acttttgcac ctgttactga ataagtaata gtgttcggcg tagtactatt
    15961 accggtaaag gagatagagg taccgccaat gtcaatggtt gtcgctttgg tattattggc
    16021 tgcaagtgtg gacagactag cctcagtcaa agaagcacta aagccgtttt tcgttaagcg
    16081 cccagttgta tcaatgtaaa gtttatctcc attgcttgac gtaattttac catcactgct
    16141 gatctgaaca tcctgtgcag aaccatcgat agtaatagtg cccttagcgg tatcaccaac
    16201 gcccggggtc aaatatttct gaacatcggc agctgaagcc gttgtggtat caaaagagta
    16261 agacttactt gcactgtcat acttgtaatt cgttgcacta gccgccgttc cgaagccgtt
    16321 attaatggtt gcctgaacag tatcaccatc actaactcca gccagcaaat cagacgcttt
    16381 agcagcatcg taacccgcac tcacagtata tttgttgcct accacagttg cattagcagc
    16441 taccaagtca gctttagatg cagcagtgtt agcaacagcc ccgccaccat tcacattaaa
    16501 cccactcagc cccagcgtat ctgagtcaat tttcttcaga tcaatcgtga tagtctggcc
    16561 gtcattcgca ccaacctgaa ttttcatgga accgtctttc gccagtacgt tcacgccgtt
    16621 gaactgggtc tggccagata cgcggtcaat ttcgtccaga cgggatttga tttcgtcctg
    16681 aatggagtcc agatccgaat cggagttagt cccggtagaa gcctgaaccg tcagttcacg
    16741 gatacgctgt aagttgttgt tgatttcgga cagcgcgcct tcggtggtct gcgcaacgga
    16801 aataccgtcg ttggcgttac gtgcagcctg agtcaggcct ttaatgttag aagtaaaacg
    16861 gttagcaatc gcctgacctg cggcgtcatc cttcgcgctg ttaatacgca agccagaaga
    16921 cagacgctcg atagaactcg acagcgcaga ctggttcttg ttgatattat tttgagtgat
    16981 cagcgagagg ctgttggtat taatgacttg tgccatgatt cgttatccta tattgcaagt
    17041 cgttgattac gtattgggtt tccacccgtc ggctcaatcg ccgtcaaccc tgttatcgtc
    17101 tgtcgtaaaa caacctttag aatttttttc acaaacagcc attttttgtt agtcgccgaa
    17161 atactctttt ctctgcccct tattcccgct attaaaaaaa acaattaaac gtaaactttg
    17221 cgcaattcag gccgataacc ccggtattcg ttttacgtgt cgaaagataa aaggaaatcg
    17281 catggcaagt atttcatcgc tgggagtcgg gtcaggtctg gatttaagtt caatccttga
    17341 tagcctcacc gccgcgcaaa aagcgacgct aacccccatt tcaaatcagc aatcgtcgtt
    17401 taccgctaaa cttagcgcct acggtacgct gaaaagcgcg ctgacgactt tccagaccgc
    17461 caatactgca ttgtctaaag ccgatctttt ttccgccacc agcaccacca gcagcaccac
    17521 cgcgttcagt gccaccactg cgggaaacgc tatcgccggg aaatacacca tcagcgtcac
    17581 ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc agagacgata cgaaaacggc
    17641 gatcgccacc agcgacagta aactcaccat tcaacaaggc ggcgacaaag atccgattac
    17701 cattgatatc agcgcggcta actcgtcgtt aagcgggatc cgtgatgcca tcaacaacgc
    17761 aaaagcgggc gtaagtgcga gcatcattaa cgtgggtaac ggtgaatatc gtctgtcagt
    17821 cacatcaaat gacaccggcc ttgataatgc gatgacactc tcggtcagcg gtgatgatgc
    17881 gctacaaagt tttatgggct atgacgccag tgccagcagc aacggtatgg aggtctcggt
    17941 tgccgcccag aatgcgcagc tgacggtcaa caacgtcgcc atcgagaaca gcagcaacac
    18001 catcagcgac gcgctggaaa acatcaccct gaacctgaac gatgtcacca cgggcaacca
    18061 gacgctaacc atcactcagg acacctccaa agcgcaaacg gcgattaaag attgggtgaa
    18121 tgcctataac tcgctaatag ataccttcag cagcctgacc aaatacaccg ccgtagatgc
    18181 gggagctgat agccagagtt ctagcaatgg cgcactgctc ggcgactcca cgttgcggac
    18241 gattcagacg cagttgaaat cgatgctgag taataccatc agttcttcca actataaaac
    18301 gctggcgcag attggtatca cgaccgatcc cagcgatggc aaactggaac tggatgccga
    18361 caaactcacc gctgcactga aaaaagatgc cagcggcgta ggtgcattga ttgttggcga
    18421 tggtaaaaaa accggcatca cgaccaccat cggcagcaac ctgaccagtt ggctttcgac
    18481 aacgggcatt attaaagccg ctaccgatgg cgttagtaaa accctgaata agttaactaa
    18541 agactacaac gccgccagcg atcgcattga tgcgcaggtc gcgcgctata aagaacaatt
    18601 tacccaactg gacgttttaa tgacctcgtt aaacagcaca agcagctact taacgcagca
    18661 gttcgaaaac aacagtaatt ccaaataagc aatattcatc gggagacagg tcatgtacgc
    18721 ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag
    18781 ccagcagcag ctggtcacca tgctatttga tggagtgctg agcgcactgg ttagagcgcg
    18841 cctgtttatg caggacaaca atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa
    18901 catcattgag aacggactgc gggtgagtct tgatgaagag agcaaagacg aattaaccca
    18961 aaacttgatt gctctttata gctatatggt caggcgtttg ctgcaagcca atttacgcaa
    19021 cgatgtctcc gcagtcgaag aagtggaagc attaatgcgc aatattgccg atgcctggaa
    19081 agagtcgtta ctctcccctt ctttgattca ggacccagtc tgatgaacca tgcaccgcat
    19141 ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc tcatgttacg cctggcaacg
    19201 gaagaacaat gggacgaact catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag
    19261 attgcacatc tgactgaaga gattaaccca tccaccacga tgcaggagca gctccgcccg
    19321 atgctgcgcc tgattctcga caacgaaagc aaggtaaagc agttattaca gattcggatg
    19381 gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat
    19441 ggcgatcagg gcggctttgt gctggctccg caagataact tctcgtgaat cttaacaagc
    19501 taatggctcg cgaataatct gattacggct acgcttctaa tgttcccctt gaatggagtc
    19561 gaagaatgcg taatcccacg ctgttacaat gttttcactg gtattacccg gaaggcggta
    19621 agctctggcc tgaactggcc gagcgcgccg acggttttaa tgatattggt atcaatatgg
    19681 tctggttgcc gcccgcctat aaaggcgcat cgggcgggta ttcggtcggc tacgactcct
    19741 atgatttatt tgatttaggc gagtttgatc agaaaggcag catccccact aaatatggcg
    19801 ataaagcaca actgctggcc gccattgatg ctctgaaacg taatgacatt gcggtgctgt
    19861 tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc
    19921 gtgtaaatgc tgatgaccgt acgcaaattg acgaagaaat cattgagtgt gaaggctgga
    19981 cgcgttacac cttccccgcg cgtgccgggc aatactcgaa gtttatctgg gatttcaaat
    20041 gttttagcgg tatcgaccat atcgaaaacc ctgatgaaga tggcatattt aaaattgtta
    20101 acgactacac cggcgaaggc tggaacgatc aggttgatga tgaattaggt aatttcgatt
    20161 atctgatggg cgagaatatc gattttcgca atcatgccgt gacggaagag attaaatact
    20221 gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac
    20281 atattccggc gtggttttat aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc
    20341 cgctgtttat tgtggcggag tactggtcgc atgaagtcga taaactgcaa acgtatattg
    20401 atcaggtgga aggcaaaacc atgctgttcg atgcgccgct gcagatgaaa ttccatgaag
    20461 catcgcgcat ggggcgtgac tacgacatga cgcagatttt cacgggtaca ttagtggaag
    20521 ccgatccttt ccacgccgtg acgctcgttg ccaatcacga cacccaaccg ttgcaagccc
    20581 tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcata tgccttaatt ttgttgcggg
    20641 aaaatggcgt tccttcggta ttctatccgg acctctacgg tgcgcattac gaagatgtcg
    20701 gcggtgacgg gcaaacctat ccgatagata tgccaataat cgaacagctt gatgagttaa
    20761 ttctcgcccg tcagcgtttc gcccacggtg tacagacatt atttttcgac catccgaact
    20821 gcattgcctt tagccgcagt ggcactgacg aatatcccgg ctgcgtggtg gtcatgtcga
    20881 acggggatga tggcgaaaaa accattaatc tgggagagaa ttacggcaat aaaacctggc
    20941 gtgattttct ggggaaccgg caagagagcg tagtaaccga cgaaaacggt gaagcgacct
    21001 tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga agaggtgatt taaattcatc
    21061 cccggcggca agccggggag atttcattac ggcagtggcg tcggtaacgg cgctttatcc
    21121 agcgcggcgg cacactctgc cgtcgggcga tccacacgct ccatcgtcat accatcgtac
    21181 tcaatggtgt taccgtcacg ctcaacctcg tacagctcac gttttacggt aacgttagtg
    21241 agatcatctg acatcaatgt caacttgccc ggcacggcaa tcacccgctg ccactgacgg
    21301 caatccaggg tatcgccctc tttagtgaca atcaggctgc ctatcgcttc cggactgacc
    21361 agcgcacgtt gcggcccttt ggtctgccag tagcccgcca gccagtccgg cgcgggggtt
    21421 ttcacgacat tgttatagtt ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct
    21481 gcaattgcga gttttttcat cattcgtcct gcatgagaag aaaagaggat tgtggcatta
    21541 aagccctgat gtcgccagcc tttccaggtt cagagagaaa tttgatctaa ctattatttt
    21601 tgtgaattat taccccgaaa ataaatttgc atgatgtatg attcgcgact cttgtttcaa
    21661 accttctgag ttcagaggct acattcatgt catggcagca attcaaacac gcctggttga
    21721 ttaaattctg ggcacccatc cctgcggtca tcgcggcggg tattctctcc acttactatt
    21781 ttggcattac tggcaccttt tgggctgtca cgggtgaatt tacccgttgg ggcggccagc
    21841 tcctgcagct gtttggcgtc catgctgaag agtggggtta ttttaaaatt atccatctgg
    21901 aaggatcgcc attaacccgc atcgacggga tgatgatcct cggcatgttt ggcggctgct
    21961 ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat gccacgcagc cgtatccgca
    22021 ttatgcaggc cattattggc ggtattatcg ctggttttgg cgcgcgtctg gcaatgggct
    22081 gtaacctggc ggcgttcttt accggtattc ctcagttctc gctgcatgcc tggttctttg
    22141 ccatcgccac tgccattggt tcatggtttg gcgcgcgctt taccctgctg cccatcttcc
    22201 gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc
    22261 aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt cggcatgctg ggctgggcgc
    22321 tgctcacggc gatgaaccaa cccaaactgg ggctggcaat gctgtttggc gtcggctttg
    22381 gtttactgat tgaacgtgcg caaatctgct ttacttcagc gttccgcgat atgtggatca
    22441 ccggacgtac ccatatggcg aaagcaatca ttattggtat ggcggtgagt gccatcggga
    22501 tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg
    22561 taattggtgg tttactgttt ggttttggca tcgtgctggc aggcggctgc gaaaccggct
    22621 ggatgtaccg cgcggtagaa ggccaggtgc actactggtg ggtcggtctg ggcaacgtga
    22681 tcggctcaac gattctggcg tattactggg atgatttcgc tccggcgctg gccaccgact
    22741 gggacaaaat caacctgctg aaaacctttg gcccgatggg tggcctgctg gtgacatatt
    22801 tgctgttgtt tactgcgctg atgttgatta tcggctggga aaaacgcttc ttccgccgtg
    22861 cggcaccgca gactgctaag gagatcgcat gaaaaatatc gttcctgatt accgccttga
    22921 tatggtgggt gaaccctgcc cttatccggc agtcgcaact cttgaggcga tgccgcagtt
    22981 aaaaaaaggg gaaatcctgg aagtggtgag cgactgtcca cagtcgatca ataatattcc
    23041 actggatgcg cgtaatcacg gctatacggt gctggatatt cagcaagacg ggccgaccat
    23101 tcgttattta attcagaagt aatccctcat tcccgctgtg gcccacagcg ggaacatcgt
    23161 tacctttgta tcacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca
    23221 ggaggtttct atgtgtggac gctttgccca atcccaaacg cgtgtagatt accttgcgct
    23281 tctcgcggaa gatattgaac gcgatattcc ctatgatccc gaacccattg gcagatacaa
    23341 cgtcgcgccg ggaaccaaag tccttctgct cagtgaacgt gacgaacacc ttcatctgga
    23401 tccggttttc tggggatatg ctcccggatg gtgggataaa ccgccactga ttaacgcccg
    23461 cgtagaaact gccgcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat
    23521 ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc gacaaaaaac agccttattt
    23581 tatctatcgc gctgatggac aacctgtttt tatggccgcg ataggtagca caccgtttga
    23641 gcgtggtgac gaagccgaag gatttttaat tgtcactgct gcagcagacc agggtctggt
    23701 agacattcat gaccgccgcc cactggtact gtcgccagaa gctgcgcggg aatggatgcg
    23761 gcaagagatt ggcggtaaag aagcatcaga aattgctacc aacggctgtg tgccagcaaa
    23821 ccagttcacc tggcacccgg tatcgcgcgc ggtaggtaat gtcaaaaacc agggcgcgga
    23881 gttaattcaa cctgtttgtt gacatcctcc acgccctgaa ggacgaggat ccctgctacg
    23941 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgggcgg cctgactgca
    24001 ccatctctcc acaggcaaga acggcgtgcc cc
//
LOCUS       DAZFVO020000069        23997 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFVO020000069 DAZFVO020000000
VERSION     DAZFVO020000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.069"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..685)
                     /locus_tag="ACHKNB_RS12695"
                     /old_locus_tag="pgaptmp_002539"
     CDS             complement(<1..685)
                     /locus_tag="ACHKNB_RS12695"
                     /old_locus_tag="pgaptmp_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4196030.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHKNB_RS12700"
                     /old_locus_tag="pgaptmp_002540"
     CDS             complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHKNB_RS12700"
                     /old_locus_tag="pgaptmp_002540"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HGU4196031.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHKNB_RS12705"
                     /old_locus_tag="pgaptmp_002541"
     CDS             complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHKNB_RS12705"
                     /old_locus_tag="pgaptmp_002541"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HGU4196032.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHKNB_RS12710"
                     /old_locus_tag="pgaptmp_002542"
     CDS             complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHKNB_RS12710"
                     /old_locus_tag="pgaptmp_002542"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HGU4196033.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHKNB_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /gene_synonym="priS"
     CDS             complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHKNB_RS12715"
                     /old_locus_tag="pgaptmp_002543"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HGU4196034.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHKNB_RS12720"
                     /old_locus_tag="pgaptmp_002544"
     CDS             complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHKNB_RS12720"
                     /old_locus_tag="pgaptmp_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HGU4196035.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHTTEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACHKNB_RS12725"
                     /old_locus_tag="pgaptmp_002545"
     CDS             complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACHKNB_RS12725"
                     /old_locus_tag="pgaptmp_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HGU4196036.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAELAAGFSELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8876..10534
                     /gene="ybjD"
                     /locus_tag="ACHKNB_RS12730"
                     /old_locus_tag="pgaptmp_002546"
     CDS             8876..10534
                     /gene="ybjD"
                     /locus_tag="ACHKNB_RS12730"
                     /old_locus_tag="pgaptmp_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HGU4196037.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACHKNB_RS12735"
                     /old_locus_tag="pgaptmp_002547"
     CDS             complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACHKNB_RS12735"
                     /old_locus_tag="pgaptmp_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HGU4196038.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11638..12753
                     /gene="macA"
                     /locus_tag="ACHKNB_RS12740"
                     /old_locus_tag="pgaptmp_002548"
     CDS             11638..12753
                     /gene="macA"
                     /locus_tag="ACHKNB_RS12740"
                     /old_locus_tag="pgaptmp_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HGU4196039.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12750..14696
                     /gene="macB"
                     /locus_tag="ACHKNB_RS12745"
                     /old_locus_tag="pgaptmp_002549"
     CDS             12750..14696
                     /gene="macB"
                     /locus_tag="ACHKNB_RS12745"
                     /old_locus_tag="pgaptmp_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HGU4196040.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACHKNB_RS12750"
                     /old_locus_tag="pgaptmp_002550"
     CDS             complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACHKNB_RS12750"
                     /old_locus_tag="pgaptmp_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HGU4196041.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15143..15217
                     /gene="yljB"
                     /locus_tag="ACHKNB_RS12755"
                     /old_locus_tag="pgaptmp_002551"
     CDS             15143..15217
                     /gene="yljB"
                     /locus_tag="ACHKNB_RS12755"
                     /old_locus_tag="pgaptmp_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HGU4196042.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15316..15636
                     /gene="clpS"
                     /locus_tag="ACHKNB_RS12760"
                     /old_locus_tag="pgaptmp_002552"
     CDS             15316..15636
                     /gene="clpS"
                     /locus_tag="ACHKNB_RS12760"
                     /old_locus_tag="pgaptmp_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HGU4196043.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15667..17943
                     /gene="clpA"
                     /locus_tag="ACHKNB_RS12765"
                     /old_locus_tag="pgaptmp_002553"
     CDS             15667..17943
                     /gene="clpA"
                     /locus_tag="ACHKNB_RS12765"
                     /old_locus_tag="pgaptmp_002553"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HGU4196044.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18287..18374)
                     /locus_tag="ACHKNB_RS12770"
                     /old_locus_tag="pgaptmp_002554"
     tRNA            complement(18287..18374)
                     /locus_tag="ACHKNB_RS12770"
                     /old_locus_tag="pgaptmp_002554"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18338..18340),aa:Ser,seq:gga)
     gene            18608..19546
                     /gene="ghrA"
                     /locus_tag="ACHKNB_RS12775"
                     /old_locus_tag="pgaptmp_002555"
     CDS             18608..19546
                     /gene="ghrA"
                     /locus_tag="ACHKNB_RS12775"
                     /old_locus_tag="pgaptmp_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HGU4196045.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFSGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            19601..20338
                     /gene="ycdX"
                     /locus_tag="ACHKNB_RS12780"
                     /old_locus_tag="pgaptmp_002556"
     CDS             19601..20338
                     /gene="ycdX"
                     /locus_tag="ACHKNB_RS12780"
                     /old_locus_tag="pgaptmp_002556"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HGU4196046.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            20362..20916
                     /gene="ycdY"
                     /locus_tag="ACHKNB_RS12785"
                     /old_locus_tag="pgaptmp_002557"
     CDS             20362..20916
                     /gene="ycdY"
                     /locus_tag="ACHKNB_RS12785"
                     /old_locus_tag="pgaptmp_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HGU4196047.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            21018..21509
                     /gene="ycdZ"
                     /locus_tag="ACHKNB_RS12790"
                     /old_locus_tag="pgaptmp_002558"
     CDS             21018..21509
                     /gene="ycdZ"
                     /locus_tag="ACHKNB_RS12790"
                     /old_locus_tag="pgaptmp_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4196048.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(21573..22406)
                     /gene="csgG"
                     /locus_tag="ACHKNB_RS12795"
                     /old_locus_tag="pgaptmp_002559"
     CDS             complement(21573..22406)
                     /gene="csgG"
                     /locus_tag="ACHKNB_RS12795"
                     /old_locus_tag="pgaptmp_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HGU4196049.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(22433..22849)
                     /gene="csgF"
                     /locus_tag="ACHKNB_RS12800"
                     /old_locus_tag="pgaptmp_002560"
     CDS             complement(22433..22849)
                     /gene="csgF"
                     /locus_tag="ACHKNB_RS12800"
                     /old_locus_tag="pgaptmp_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HGU4196050.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(22874..23263)
                     /gene="csgE"
                     /locus_tag="ACHKNB_RS12805"
                     /old_locus_tag="pgaptmp_002561"
     CDS             complement(22874..23263)
                     /gene="csgE"
                     /locus_tag="ACHKNB_RS12805"
                     /old_locus_tag="pgaptmp_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HGU4196051.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(23268..23918)
                     /gene="csgD"
                     /locus_tag="ACHKNB_RS12810"
                     /old_locus_tag="pgaptmp_002562"
     CDS             complement(23268..23918)
                     /gene="csgD"
                     /locus_tag="ACHKNB_RS12810"
                     /old_locus_tag="pgaptmp_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HGU4196052.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         5919 a         5918 c         6347 g         5813 t
ORIGIN      
        1 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
       61 cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt
      121 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
      181 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
      241 cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg
      301 tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
      361 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct
      421 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
      481 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
      541 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
      601 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
      661 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
      721 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
      781 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
      841 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
      901 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
      961 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
     1021 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
     1081 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
     1141 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
     1201 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
     1261 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
     1321 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
     1381 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
     1441 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
     1501 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
     1561 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
     1621 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
     1681 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
     1741 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
     1801 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat
     1861 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
     1921 agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg
     1981 gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct
     2041 ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc
     2101 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
     2161 cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg
     2221 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt
     2281 agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg
     2341 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
     2401 ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga
     2461 aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt cgacttgata
     2521 tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca
     2581 atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt
     2641 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg
     2701 gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
     2761 acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
     2821 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
     2881 aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg cgtggttgcc
     2941 ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg
     3001 ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa
     3061 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
     3121 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
     3181 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg
     3241 gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact
     3301 tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga
     3361 ccgttcagag agtcgcctgt gactccccag atgcgtttca cccctgccga ttcgagtgtt
     3421 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
     3481 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
     3541 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
     3601 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
     3661 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
     3721 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
     3781 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
     3841 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
     3901 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
     3961 acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg
     4021 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
     4081 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
     4141 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
     4201 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
     4261 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
     4321 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
     4381 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
     4441 cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
     4501 gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
     4561 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
     4621 cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
     4681 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct
     4741 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct
     4801 ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga cctgcatcta
     4861 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
     4921 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
     4981 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
     5041 ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga ccaaagccca
     5101 cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
     5161 tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc
     5221 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
     5281 tcacgatggg gccagggaaa cgagcgaact ctacttgctg attctgccag ccgctgccgt
     5341 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
     5401 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
     5461 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct
     5521 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
     5581 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
     5641 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
     5701 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
     5761 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
     5821 ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
     5881 tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg
     5941 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
     6001 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
     6061 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
     6121 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
     6181 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
     6241 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
     6301 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
     6361 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
     6421 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
     6481 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
     6541 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
     6601 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
     6661 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
     6721 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
     6781 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
     6841 taacgtgtat tcactggctt cggtcgtgtg ttgtaagaaa gccagtccac tcagaccaat
     6901 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
     6961 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
     7021 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
     7081 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
     7141 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
     7201 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
     7261 gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcta tgacacacta ctgaaagcgg
     7321 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag tagagtattc
     7381 gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt tggtggcgtc
     7441 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
     7501 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agtcgagggc
     7561 accgcgcagc ggcgattttg ttcgccagag cccgggggtg catggggcgg cggcgattgg
     7621 ccgccccctg cgcgctcctt gcgccagtgg caatatgtgg cttagctcat gaaaggagcg
     7681 caacaagatg atgaatcaac atataacaac atcttaaaaa aaggcctgac attacgccag
     7741 gccttctgcg ttaattaatc acgcttttcc agcagggtcc ggtaaatcag accaccgata
     7801 atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc
     7861 tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
     7921 ctaattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccggc
     7981 gcgaatttgt cggttgcgcc gtggatcacc aacaggaaac ctgcactcaa taccagttca
     8041 actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa
     8101 gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc
     8161 gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
     8221 ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
     8281 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaacgccaa cgccacgccg
     8341 gcaaaaccaa tgcctaattc cgagaagcct gcggccagtt cagcactacc acagccacca
     8401 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
     8461 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaagtgac
     8521 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
     8581 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
     8641 cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
     8701 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
     8761 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcaagg
     8821 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
     8881 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctcga
     8941 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
     9001 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
     9061 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
     9121 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
     9181 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
     9241 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgtcgagga
     9301 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
     9361 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gttcccaatg tggaagtcac
     9421 cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa atctctctga
     9481 tggacagatt cgtcagggac tttccgcaat ggtgcagttg cttgagcatt atttctctga
     9541 gcagggggct ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
     9601 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
     9661 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgagact
     9721 ggataaagac gcgcgtccgt tattgctaat tgaagatcca gaaacccgcc tgcatccgat
     9781 tatgctttcc gttgcctggc atctgttgaa tcttttgcct ttacagcgta ttgctaccac
     9841 caactctggc gagctgcttt cgttaactcc agtggaacat gtttgccgcc tggtgcgtga
     9901 atcttctcgt gttgccgcct ggcgtctggg gccaagtggc ttgagcaccg aagacagccg
     9961 ccgcatctct tttcacattc gttttaatcg tccgtcatcg ctgtttgctc gctgctggtt
    10021 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
    10081 tcattttgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    10141 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    10201 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    10261 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    10321 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa
    10381 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    10441 ggaggccgga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
    10501 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    10561 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    10621 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    10681 tacgccgcca accgattccc agaaagcgtt gtaatcggca tggatcttgc cttctttatc
    10741 acgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    10801 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    10861 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    10921 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    10981 tgccagagga ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat
    11041 tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc
    11101 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg
    11161 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    11221 aaggtaaggg cgatgcagac gcacaggcag tcgcggttgg cgcgttaaca acacattgag
    11281 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
    11341 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
    11401 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    11461 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
    11521 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tatggttttg
    11581 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
    11641 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    11701 ctgattacgt tgtggagaat tcttaacgca cccgtgccga cttatcagac gctgattgtg
    11761 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    11821 gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat
    11881 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
    11941 aaggaggtcg aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag
    12001 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggctgtt
    12061 tcacagcagg atctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    12121 accattgacg cgcaaatcaa gcgcaatcag gcttctctcg atacggctaa aaccaatctc
    12181 gattacactc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
    12241 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
    12301 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    12361 aaagcctggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    12421 gtactaccga cgccggaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc
    12481 cccaatccca atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc
    12541 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    12601 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    12661 gcacgtaacg ataccgatgt tgagattgtc aaagggcttg aagcgggcga tgaagtggtg
    12721 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    12781 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc agcctcgata
    12841 tttatgcggg tgagatggtc gcgattgttg gcgcttcggg ttccggtaaa tcgaccctga
    12901 tgaatattct cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg
    12961 atgttgccac gctggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    13021 ttttccagcg ttaccatttg ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    13081 ccgtctatgc tggtcttgag cggaaacagc gactgcttcg tgcccaggag ttgctgcaac
    13141 ggctggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    13201 agcgcgtcag catcgcgcgg gcattgatga acggtggtca ggtaattctt gccgatgaac
    13261 caaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    13321 gcgatcgtgg gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    13381 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    13441 tgaatgttgc tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg
    13501 tcagcggttt taacgaggcg ctgacgatgg catggcgggc actggcagcg aataaaatgc
    13561 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    13621 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctata ggtacgaata
    13681 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    13741 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    13801 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    13861 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    13921 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    13981 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    14041 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    14101 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggcttaact
    14161 ccattactgt cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    14221 gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg gacggcgtct
    14281 tgaaaactgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
    14341 tttcgctggt ggtgggtggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    14401 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    14461 agttcctgat cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
    14521 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    14581 cgttggcact gctgctggcg tttctctgct cgacggccac cgggatttta tttggctggt
    14641 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt
    14701 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    14761 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    14821 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    14881 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    14941 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    15001 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    15061 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcatt
    15121 tgacagggga cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    15181 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtgaagtag
    15241 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
    15301 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
    15361 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
    15421 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
    15481 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
    15541 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
    15601 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
    15661 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
    15721 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
    15781 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
    15841 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
    15901 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
    15961 ggtcgcaatg aggtaaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
    16021 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc
    16081 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
    16141 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
    16201 cttgcgcgcg tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
    16261 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
    16321 ggtaaaaccg cgattgcgga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa
    16381 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcactaaa
    16441 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
    16501 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
    16561 ggccaggttg atgcggctaa cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta
    16621 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
    16681 cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc
    16741 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
    16801 cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
    16861 attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
    16921 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag
    16981 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
    17041 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
    17101 gcaggtttag gtcacgaaca taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg
    17161 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc
    17221 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
    17281 ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa gcatccacat
    17341 gcggttctgc tgctggacga aatcgagaaa gcgcaccctg acgtgttcaa tattctgttg
    17401 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
    17461 gtggtgctgg tgatgaccac caacgccggg gtacgtgaaa ctgagcgtaa atccattggt
    17521 cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat ctttacaccg
    17581 gaattccgta accgtctcga caacattatc tggtttgatc atctgtcaac cgacgtgatc
    17641 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    17701 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    17761 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    17821 gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag
    17881 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    17941 taatctgatt gttaggtagg ttggtcaagt ccgtaatctc gaaagaggtt acggactttt
    18001 tgtttatggg gtggaggagg ttcagaccct ttttttaatg atgatggtaa gttgttgata
    18061 attagtgctg cgggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg
    18121 ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag
    18181 aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca gacagataaa
    18241 atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg
    18301 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
    18361 tcggacacct caccaaattg tcgttcctgt cttgctggaa cgggcgctaa tttagggaaa
    18421 tcacgacctg aggtcaacaa actttttgaa aaaatcgcgc gtttattcaa acttcaatca
    18481 atgtgtggtt ttaataagcg aaatctgctt tttttgccac cgaccacgga tttgttatgc
    18541 tggtggcctt tgtagatcat aacgataagt gcgaataaat ttcgcacaac gcttttcggg
    18601 agtcagtatg gatatcatct tttatcaccc aacgttcgat acccaatggt ggattgaggc
    18661 actgcgcaaa gctattcctc aggcaagagt cagagcatgg aaaagcggag ataatgactc
    18721 tgctgattat gctttagtct ggcatcctcc tgttgaaatg ctggcagggc gcgatcttaa
    18781 agcggtgttc gcactcgggg ccggtgttga ttctattttg agcaagctac aggcacaccc
    18841 tgaaatgctg aacccttctg ttccactttt tcgcctggaa gataccggta tgggcgagca
    18901 aatgcaggaa tatgctgtca gtcaggtgct gcattggttt cgacgttttg acgattatcg
    18961 catccagcaa aatagttcgc attggcaacc gctgcctgaa tatcatcggg aagattttac
    19021 catcggcatt ttgggcgcag gcgtactggg cagtaaagtt gctcagagtc tgcaaacctg
    19081 gcgctttccg ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg tgcaaagctt
    19141 ttccggacgg gaagaactgt ctgcatttct gagccaatgt cgggtattga ttaatttgtt
    19201 accgaatacc cctgaaaccg tcggcattat taatcaacaa ttactcgaaa aattaccgga
    19261 tggcgcgtat ctcctcaacc tggcgcgtgg tgttcatgtt gtggaagatg acctgctcgc
    19321 ggcgctggat agcggcaaag ttaaaggcgc aatgttggat gtttttaatc gtgaaccctt
    19381 accgcctgaa agtccgctct ggcaacatcc acgcgtgacg ataacaccac atgtcgccgc
    19441 gattacccgt cccgctgaag ctgtggagta catttctcgc accattgccc agctcgaaaa
    19501 aggggagagg gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa gcatcaggat
    19561 tcctgctatc cttggcggga attgaataca ggagagagtt atgtatcccg tcgaccttca
    19621 tatgcatacc gttgccagca cacatgcata tagcacattg agtgattaca ttgcccaggc
    19681 caaacaaaag ggcattaaac tttttgcgat caccgatcat ggcccggata tggaagatgc
    19741 tccgcatcac tggcacttca ttaacatgcg tatctggccg cgagtggttg atggggtagg
    19801 gatcctgcgc ggcatcgaag ctaacattaa aaatgttgat ggtgaaattg attgcagcgg
    19861 taaaatgttt gactcgctgg atctgattat tgccggtttt catgagccgg tttttgcacc
    19921 acatgacaaa gcgaccaata cacaagcgat gatcgccact atcgccagcg gcaatgtgca
    19981 tataataagc catcccggaa atcccaaata tgaaatagat gtgaaagccg ttgctgaagc
    20041 agccgcgaaa catcaggtgg cgctggaaat caataattct tcatttttac actcacgtaa
    20101 gggcagtgaa gacaactgtc gtgcggtagc cgcagcggta cgtgatgctg gaggttgggt
    20161 ggcattaggc tcggattctc acaccgcgtt taccatgggg gaatttgaag agtgtcttaa
    20221 aatcctcgac gcggtagatt ttccgccaga gcgcattttg aatgtttctc cgcgccgctt
    20281 actgaacttc cttgaatctc gcggtatggc accgattgcg gaatttgcag acctttaatt
    20341 actagtaatg gaaatatatt aatgaacgag ttttctatcc tctgtcgtgt gctgggttcg
    20401 ctctattacc gccaaccgca agatccttta ctggtgccgc tgtttaccct gattcgtgag
    20461 gggaaactgg ctgcgaactg gccactggag caggatgagt tactgacgcg tttacagaaa
    20521 agctgtgata tggcgcaagt ctctgccgat tacaatgcgt tgtttatcgg cgatgaatgt
    20581 gccgtgccgc catatcgtag cgcatgggtt gaggacgcaa cggaagcgga agtgcgcgct
    20641 tttctttccg aacgagggat gccactggcg gatacgccag ccgatcacat cggcacattg
    20701 ctgcttgcgg cttcctggct ggaagatcag tcaacggaag atgagagcga agcactggaa
    20761 acactgttca gtgagtatct gttaccctgg tgtggtgcgt tcctcggcaa agtggaggcc
    20821 catgcaacca cgcctttctg gcgcaccatg gcaccgctaa cccgtgatgc tattagtgca
    20881 atgtgggacg agctggaaga agattctgaa gagtaaatgt gatcatcgtc actcataact
    20941 gcaacagata atttgttgtt gcataaaatg tgtgctcgat ctcattcatg gccgcgtttt
    21001 ctgctatcat gcgcggcatg aacatacttc tctctattgc aatcacaaca ggcattctct
    21061 ccggtatctg gggatgggtg gctgtttccc tgggcttact aagctgggcg ggcttcctgg
    21121 gctgtacggc ctactttgcc tgcccgcaag gtgggctgaa agggctggcg atctccgctg
    21181 caaccctgct tagtggcgtg gtgtgggcga tggtcattat ttacggtagt gcactggcac
    21241 cacatctgga aattctcggt tatgtcataa ccggtattgt cgcgtttctg atgtgtattc
    21301 aggccaaaca gctgctgctt tcatttgttc cgggaacgtt tataggcgca tgcgcgacgt
    21361 ttgccgggca gggtgactgg aaactggtgt taccttcgct ggcgcttggg ctggtatttg
    21421 gttacgcaat gaaaaacagc ggcctgtggc tggcggcgcg tagtgcaaag acggcgcacc
    21481 gtgagcagga aatcaaaaat aaagcgtgag gggcactcac gctttcgctt aaacagtaaa
    21541 atgccggatg ataattccgg cttttttatc tgtcaggatt ccggtggaac cgacatatgg
    21601 cggtatttca ccagaatgtc gttctgccgt tctgctttgt tctgcaaatc ccacagacca
    21661 cggtcgatac catcattaat caggaaaatg acccctgttt cgatagccga catcagacac
    21721 agcataacgg gttcgtttga ggtgtaaccc acttcccctt ccagcaagcg ctggtagtca
    21781 ataaagcgga aaaccccggc ctgaacttca taggaaagta tcgtcttact ggtgttcacc
    21841 gaggagagga tctcaccggt actcacattg acgacacgca ggttcacggc aatctgatcg
    21901 agctggtatt gcgtgtctgc accgatccca aaatatcttg ccccaacacc gcccgattta
    21961 acgttgcttt cataaccgat aatcgaacct tcaaccataa tatttgccgc cgttaaagat
    22021 tgcagcggga ttcggttatt aatggcaacc gtgccgtttt cttgtgccgc acgaataatc
    22081 ttgcgctcgt taagcaggtt ttgtaagccc tggcgctcca gcggtataaa ccagcgagaa
    22141 tctttcaatg ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt ggagaagtta
    22201 cttgccgggt agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac cgaaacaaag
    22261 atcttacccg tcggcgctgg cagatgggtc aaatctttgt agctctgagc tcgaggcatt
    22321 aatgtcggtc tggcggcttc tttaggcggg gcggttaagc atccgctcag taacatgacg
    22381 gcaaccaaaa gaaataagcg ctgcatgatt attttcctta tgaagctggg gcttaaaaat
    22441 cggttgagtt attttgtaaa cccgaaacct ggatggtcga ggtttgtccg gttttacgat
    22501 ctgtcacgtt caactgcaat tgaccatcgc ggttagcaat atcaacaata taatcgttgg
    22561 tcaccatgcg gcccggttta ccggtattaa tattcgacag tagcccacct aaaatttgtg
    22621 actggatggc ctgagtaaag ttatctaacg ctgagggtgt ttcaatacca aagtcatcgt
    22681 tatagctcgg atctttataa gagttttggg cctgagcgct atttaataaa aaagcgccat
    22741 tatttgggtt accaccaaag tttggattac ggaactggaa agtcatggtt ccagcccaac
    22801 ttaatggcga aataagcatg agtagaacta ctgcatgttt gacacgcatt gcagcctccg
    22861 aacaattttt tatttagaat tcatcatgcg ccaaatcgcc cgtacttaat aacgcctgat
    22921 ttatctggcg acgatttagt gcttcttcag tttgaatcag tgcaaagacg acagttttct
    22981 cgaagtctct tttcaacgga aataaaaaag tctggaaaat aacgtcctga ttgaccgtta
    23041 tagtgatcca gcttccccat cgtgcactgg gcctttcatt aatcgttaag ttacccgtat
    23101 agtcactttc ccatttatca ctaaaggctc ggtaaaaatc atggccaata gatgaaacag
    23161 tatggtcagt taacaatccc gggacttcta cctcaacggc gtgaagattc cctgcggcga
    23221 acagaaattc tgccgccaca atccagcgta aataacgttt catggcttta tcgcctgagg
    23281 ttatcgtttg cccaggaaac cgcttgtgtc cggtttttta cggctatctt cttgaaaaga
    23341 ttataaagat gcgttttaac cgtattttcg ctgatgaaca acgaacgagc gatctcgtta
    23401 ttagacgcgc cgatacgcag cttattcagg atctcttttt cccgatgagt aaggagggct
    23461 gattccgtgc tgttataacg atagttacca gaatgtgtaa tcaggtaact ggcaagcttt
    23521 tgcgtaaagt agcattcgcc gcgcaggacg ccttgcaacc cattgacaac acgttcttga
    23581 tcctccatgg cataaaaaac gccgttgata tgaggccagt tttcaatgtc gcggtacggg
    23641 taatcttcag gcgtatttag caacaatatt ttgatattgt tgtttttcct gctcaaagta
    23701 tcctgccaat aatggataag ctttttatct gcttccatca tatccagaag aataatagag
    23761 cctgaagata tatcgtccag agaacgttga atattatgta attttcctgt aattgccagc
    23821 gattgtttaa ggtgctgcaa gagagctgtc gcttgcaaag acggtttagt gatcaacaat
    23881 aatgtatgac catgaatact atggacttca ttaaacatga tgaaaccccg ctttttttat
    23941 tgatcgcaca cctgacagct gcctctaaaa tagaagcacc agaagtactg acagatg
//
LOCUS       DAZFVO020000070        23897 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFVO020000070 DAZFVO020000000
VERSION     DAZFVO020000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.070"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            50..229
                     /gene="ccgAI"
                     /locus_tag="ACHKNB_RS12815"
                     /old_locus_tag="pgaptmp_002563"
     CDS             50..229
                     /gene="ccgAI"
                     /locus_tag="ACHKNB_RS12815"
                     /old_locus_tag="pgaptmp_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CcgAI"
                     /protein_id="HGU4196053.1"
                     /translation="MTTVTTPSQLKKEVESQKELLLRACLEAFNQLPNQRLQGAFPST
                     YALAAKLDQLLQQTK"
     gene            284..604
                     /gene="ccgAII"
                     /locus_tag="ACHKNB_RS12820"
                     /old_locus_tag="pgaptmp_002564"
     CDS             284..604
                     /gene="ccgAII"
                     /locus_tag="ACHKNB_RS12820"
                     /old_locus_tag="pgaptmp_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CcgAII"
                     /protein_id="HGU4196054.1"
                     /translation="MTTQTLEQTLEDFRRQCESFAREQQPRCGLIYELYQRRLSADID
                     GYLAGVPAEYREELIAVARREFDYLTQDEIAEEIRQDRENGYCSHGIERNCCPLGCGD
                     LDYY"
     gene            complement(719..1063)
                     /locus_tag="ACHKNB_RS12825"
                     /old_locus_tag="pgaptmp_002565"
     CDS             complement(719..1063)
                     /locus_tag="ACHKNB_RS12825"
                     /old_locus_tag="pgaptmp_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196055.1"
                     /translation="MNNRQRELARIRQARRRARLKEEGTSVTVTITKQEEAMLQELCR
                     VRRPGRTPYSTNEFFQLLLIRNWQQWQEQKAQLGKCQACGKLKAEGGCGGERKGETFN
                     CWLAVEANELNL"
     gene            complement(1263..1631)
                     /gene="stbC"
                     /locus_tag="ACHKNB_RS12830"
                     /old_locus_tag="pgaptmp_002566"
     CDS             complement(1263..1631)
                     /gene="stbC"
                     /locus_tag="ACHKNB_RS12830"
                     /old_locus_tag="pgaptmp_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbC"
                     /protein_id="HGU4196056.1"
                     /translation="MEKQPDKLEVLMDWFLGDAKEITATQKEMTQKLSELSEKLAKDT
                     ESLGETADSLKRALVENQRSISLAISDDAKAREEFLTKFRRAQASSAETFTRQILFIT
                     AGCTIVGAAVGAAIAILLLR"
     gene            complement(1633..2349)
                     /gene="stbB"
                     /locus_tag="ACHKNB_RS12835"
                     /old_locus_tag="pgaptmp_002567"
     CDS             complement(1633..2349)
                     /gene="stbB"
                     /locus_tag="ACHKNB_RS12835"
                     /old_locus_tag="pgaptmp_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HGU4196057.1"
                     /translation="MKVAVINYSGSVGKTLISSYLLAPRLTGAKFYAVETINQSASDL
                     GIEDVTSFKGDDFSRLIEDIVFEDAGIIDIGASNVEAFLMAMSRFDSGANEFDKYVIP
                     VTPDNKAIDESLKTAHTLSKAGVSSKKIIFVPNRISPDSEVEDVLAPVFEFVKETKIG
                     KISKKAVIYNSEVFEYLAFHRISFEVLTAEDPEEFKSRAKQTTDADERKKLARRYTYM
                     KQAIPVKANLDKAYAALMGE"
     gene            complement(2358..2789)
                     /gene="stbA"
                     /locus_tag="ACHKNB_RS12840"
                     /old_locus_tag="pgaptmp_002568"
     CDS             complement(2358..2789)
                     /gene="stbA"
                     /locus_tag="ACHKNB_RS12840"
                     /old_locus_tag="pgaptmp_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="HGU4196058.1"
                     /translation="MNKPADLKPRNLSAAVRLRLDEIENWLDRGITRHEIAEILGSEY
                     SFSVTAKGLEMALYRARQNRKSVLHNTQDSKPDDADSVLHNTQGKSSAKGTEESVLHN
                     TQPSEPEAQESEKVESPGIIDKEFFNKIGEDFDPKKFNKKF"
     gene            3451..3741
                     /locus_tag="ACHKNB_RS12845"
                     /old_locus_tag="pgaptmp_002569"
     CDS             3451..3741
                     /locus_tag="ACHKNB_RS12845"
                     /old_locus_tag="pgaptmp_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001471781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traK protein"
                     /protein_id="HGU4196059.1"
                     /translation="MKIVADQLSDVNRKLDYLFDRASDADFGSLRDELKAITETLSGV
                     KFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER"
     gene            3743..5272
                     /locus_tag="ACHKNB_RS12850"
                     /old_locus_tag="pgaptmp_002570"
     CDS             3743..5272
                     /locus_tag="ACHKNB_RS12850"
                     /old_locus_tag="pgaptmp_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4196060.1"
                     /translation="MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYL
                     VKNTFSLWPLWSALIAGWFIGIGGLVAFIIYDKSRVFKGERFKKIYRGTELVRARTLA
                     DKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKTTIFNELLFKSIIRGGKNIAL
                     DPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQESPDMATEE
                     WFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLIGTPAEAIFSGSEK
                     AVGSARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWL
                     DSIFSIVLGMGEKESRINVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLV
                     KVYGRDMAQTILANMRSNIVLGGSRLGDETLDQMSRSLGEIEGEVERKESDPQKPWIV
                     RKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKAKHVKYHRKNPVPGIELRE
                     I"
     gene            5272..8508
                     /gene="mobF"
                     /locus_tag="ACHKNB_RS12855"
                     /old_locus_tag="pgaptmp_002571"
     CDS             5272..8508
                     /gene="mobF"
                     /locus_tag="ACHKNB_RS12855"
                     /old_locus_tag="pgaptmp_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HGU4196061.1"
                     /translation="MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEAL
                     GLSGDVESARFKELLVGEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGD
                     KTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLH
                     THAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNT
                     FDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREE
                     IHQEWASRAKTLGIDFDNREWQGHGKPLEADIARNAAPDFTSPEVKADRAIQFAVKSL
                     SERDASFERQKLIQIANKQVLGHATMADVEKAYLKAVQKGAIIEGEARYQSTLKVGAS
                     VMAETLTRKEWIDSLTNSGMRADKARFAVDDGIKNGRLKKTSHRVTTVEGIRLERSIL
                     TIESRGRGQMPRQLTTEIAGQLLAGKTLKQEQMRAVTEIVTSKDRFVAAHGYAGTGKS
                     YMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLDEKSVVF
                     IDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKAGMQTNYMK
                     DIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQD
                     ATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIII
                     PEQDYKNGMKRGESYQVLDTGPGNKLTVESSSGEQIAFSPRTHTKLSVYQAVSAELAP
                     GDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVELDKRQASYLSYAYATT
                     VHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVSRDSP
                     KTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGT
                     DYQPVSNAEDAFHLKQNPMDDSVGLRRHETQQNDAELAHDYAAADDQQWSAQEYADYE
                     HYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI"
     gene            8508..9134
                     /locus_tag="ACHKNB_RS12860"
                     /old_locus_tag="pgaptmp_002572"
     CDS             8508..9134
                     /locus_tag="ACHKNB_RS12860"
                     /old_locus_tag="pgaptmp_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6710 family protein"
                     /protein_id="HGU4196062.1"
                     /translation="MEQTDKRKQDKLKFDRVINLARRLPQPAIHDLLRALILPIQADY
                     LLAVGTEGQDARPDMNEREFFFTKIIWAMDYTHMKSLRLAAEDFPLALATAKILPWPW
                     GESSYRSALADIGSAKGNPWVQDINHRVTLWLPWRIGFVRGGNHSIASGVLAGEGEVI
                     PDTVYDMRYLLDIVSTDGYYWYMSGKICERVSDYRTAAFFEIGRLLTL"
     gene            complement(9308..9841)
                     /locus_tag="ACHKNB_RS12865"
                     /old_locus_tag="pgaptmp_002573"
     CDS             complement(9308..9841)
                     /locus_tag="ACHKNB_RS12865"
                     /old_locus_tag="pgaptmp_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HGU4196063.1"
                     /translation="MKKLATWLLVATFTTAALPAFALEPSVQVGYSPEGSARVLVLSA
                     IDSAKTSIRMMAYSFTAPDIMKALVAAKKRGVDVKIVVDERGNTGRASIAAMNYIANS
                     GIPLRTDSDFPIQHDKVIIVDNVTVETGSFNFTKAAETKNSENAVVIWNMPKLAESFL
                     EHWQDRWNRGRDYRSSY"
     gene            complement(9841..10836)
                     /locus_tag="ACHKNB_RS12870"
                     /old_locus_tag="pgaptmp_002574"
     CDS             complement(9841..10836)
                     /locus_tag="ACHKNB_RS12870"
                     /old_locus_tag="pgaptmp_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HGU4196064.1"
                     /translation="MTDAAFYQLGPLREYLEDPTVFEIRINRFQEVICDTFSGRRVVQ
                     NAAITADFIRNLAKSLVSSNKLTMQAINDVILPGGIRGVICLPPAVIDGTTAVAFRKD
                     LAADKNLEQLTSEGIFSDCRKITGSKQSLTDDDFFLKELHSSEKWPAFLQTAVEKKRT
                     IVICGETGSGKTVLTRALLKSLHKDERVIILEDVHEVTVDHVIEAVYMMYGDAGKIGR
                     VSATDALRACMRLTPGRIIMTELRDDAAWDYLKALNTGHPGGVMSTHANSARDAFNRI
                     GLLIKATPIGRMLDMSDIMRMLYSTIDVVVHMEKRKIKEIYFDPEYKMQCVNGSL"
     gene            complement(10878..12038)
                     /gene="virB10"
                     /locus_tag="ACHKNB_RS12875"
                     /old_locus_tag="pgaptmp_002575"
     CDS             complement(10878..12038)
                     /gene="virB10"
                     /locus_tag="ACHKNB_RS12875"
                     /old_locus_tag="pgaptmp_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HGU4196065.1"
                     /translation="MARKSVDVDQELDENTGDGEFESERGGFKGSNRRSAPGMKAFVI
                     LMALLALVFIGITVMGKIRAPAKAEADKDGGKAQQANTLPNYSFNSDPDVNKPATAQN
                     SATDARAVQAAAQADADAGSSNTGARTSNKRKEPSPEELAMQRRLGGELAQTNQAATS
                     NSPGVQPQDNETSEGSSALAKNLTPARLKASRAGVMANPSLTVPKGKMIPCGTGTELD
                     TTVPGQVSCRVSQDVYSADGLVRLIDKGSWVDGQITGGIKDGQARVFVLWERIRNDQD
                     GTIVNIDSAGTNSLGSAGIPGQVDTHMWERLRGAIMISLFSDTLTALVNQTQSNNIQY
                     NSTENSGEQLASEALRSYMSIPPTLYDQQGDAVSIFVARDLDFSGVYTLADN"
     gene            complement(12038..12922)
                     /locus_tag="ACHKNB_RS12880"
                     /old_locus_tag="pgaptmp_002576"
     CDS             complement(12038..12922)
                     /locus_tag="ACHKNB_RS12880"
                     /old_locus_tag="pgaptmp_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HGU4196066.1"
                     /translation="MKKLLLSAVVLSVLGGAATNVMALEVGRNSPYDYRIKSVVYNPV
                     NVVKIDAIAGVATHIVVAPDETYITHAFGDSESWTFAHKMNHFFVKPKQAMSDTNLVI
                     VTDKRTYNIVLHFIGEETKKNADGTVSKSFIETPWAVRQAVLQLTYEYPFEQQEKAKS
                     AADKKRITQKLKQTAFAGAKNYQYVMSEQPEMRSIQPVHVWDNYRFTRFEFPANAELP
                     QVYMISASGKETLPNSHVVGENRNIIEVETVAKEWRIRLGDKVVGVRNNNFAPGAGAV
                     ATGTASPDVRRVQIGEDN"
     gene            complement(12933..13631)
                     /locus_tag="ACHKNB_RS12885"
                     /old_locus_tag="pgaptmp_002577"
     CDS             complement(12933..13631)
                     /locus_tag="ACHKNB_RS12885"
                     /old_locus_tag="pgaptmp_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HGU4196067.1"
                     /translation="MRANKKTGLTREAIKEFNESRKGLEVDLMDEVLKSRRTAWMVAT
                     GSAVVTVFALSLVGYVVHKYSQPIPAHLLTLNEATHEVQQVKLTRDQTSYGDEIDKFW
                     LTQYVIHRESYDFYSVQVDYTAVGLMSTPNVAESYQSKFKGRNGLDKVLGDSETTRVK
                     INSVILDKPHGVATIRFTTVRRVRSNPVDDQPQRWIAIMGYEYKSLAMNAEQRYVNPL
                     GFRVTSYRVNPEVN"
     gene            complement(13621..13767)
                     /locus_tag="ACHKNB_RS12890"
                     /old_locus_tag="pgaptmp_002578"
     CDS             complement(13621..13767)
                     /locus_tag="ACHKNB_RS12890"
                     /old_locus_tag="pgaptmp_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HGU4196068.1"
                     /translation="MRSLLLMGVLLISACSSGHKPPPEPDWSNTVPVNKTIPVDTQGG
                     ANES"
     gene            complement(13850..14890)
                     /locus_tag="ACHKNB_RS12895"
                     /old_locus_tag="pgaptmp_002579"
     CDS             complement(13850..14890)
                     /locus_tag="ACHKNB_RS12895"
                     /old_locus_tag="pgaptmp_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HGU4196069.1"
                     /translation="MAFTLVADIFAKVDGAITSMVSANVATIISDVTPLIATCLTIKL
                     MVQGMYSAFNPGAGDSLSSLIKEYLSIALILSFATAGGWYQQELVNVALHLPDDFAGI
                     LSAPNKVGASGVPAIIDSGIEKGIKIVNTAWEAADVFSSSGLAAYAIGGIMMIATVVL
                     GGLGAGFVIMAKILLAVTLCFGPIAIFCLLWGATKNIFARWLASVINYGLVVVILALV
                     FGFIMQMFDNLLSSMNSDAAYSSITGSIAALLLTVISVFVLFQIPQIAASWGSGISAG
                     VADAARSTGSSMQALGNMGSHGMFGGNAFRGGNSGGGQQSAGGGSGSNSGGNSGSNLS
                     GKARGSRGKKAA"
     gene            complement(14906..15133)
                     /gene="eexN"
                     /locus_tag="ACHKNB_RS12900"
                     /old_locus_tag="pgaptmp_002580"
     CDS             complement(14906..15133)
                     /gene="eexN"
                     /locus_tag="ACHKNB_RS12900"
                     /old_locus_tag="pgaptmp_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncN-type entry exclusion lipoprotein EexN"
                     /protein_id="HGU4196070.1"
                     /translation="MKKLLLVIPFLLVACDASHDVEWYKKHEKERKATIQECKKDADE
                     LQKPDCKNAREADRQLFVFGKKDGEINSPKI"
     gene            complement(15141..15854)
                     /locus_tag="ACHKNB_RS12905"
                     /old_locus_tag="pgaptmp_002581"
     CDS             complement(15141..15854)
                     /locus_tag="ACHKNB_RS12905"
                     /old_locus_tag="pgaptmp_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HGU4196071.1"
                     /translation="MKKTLTAVLLTTGLILGGAQSASAGIIVTNPTELAKQVEQLQQM
                     AQQLEQLKSQLQTQKNMYESMAKTTDLGDLLGTSTSTLANNLPDNWKEIYSDAMNSSS
                     SVTPSVNSMMGQFNAEVDDMTPSEAITYMNKKLAEKGAYDRVMAEKAYNNQMQELTDM
                     QELTEQIKTTPDLKSIADLQARIQTSQGAIQGEQAKLNLMNMLQQSQDKLLRAQKERA
                     THNFVFGTGGDVTASPSIN"
     gene            complement(15872..18472)
                     /locus_tag="ACHKNB_RS12910"
                     /old_locus_tag="pgaptmp_002582"
     CDS             complement(15872..18472)
                     /locus_tag="ACHKNB_RS12910"
                     /old_locus_tag="pgaptmp_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HGU4196072.1"
                     /translation="MRAATATKPKKIDAFRKEPSVNKKYLPYSYHLNDYVISMENGDL
                     MAFFKLDGRTHDCASDRELVTWHKDLNTLVKSFGTDQVELWTHEYHHEAKEYPDGEYD
                     QFFPAYVDQYNRKLHGDSKQLINDLYLTVIYKQVGDQTQKFLAKFEKPTRDQIQQMQN
                     EALEGLEDVSEQILEAMKPYGIQQLGIYYRDKRGVEIPAPDKKEREELAEVDESDVFD
                     EAVVIERNEPEPSQAHAYSKALEFLYFLANMEWAIVPVCRERIRDYIMDNRPVSSLWG
                     DVVQIRTVDHNFYTTGIEFRDYEEDTEPGQLNMLKEADFEYLLTQSFSCLSESSAKTF
                     LTHQEKSLQETRDRALSQLAQLGTALDMLTSREFVMGYHHGTVHVWDNDQNAVQRKAR
                     RVKVMLTGCGVVGGTISLASEAAYYAKLPGNQKWAPRPVPVNSWNFLHFSSFHNFMRG
                     KPDNNPWGPALTMFRTISGTPLYFNFHVTPLEELSYGKRPLGHALITGMSGEGKTTLL
                     NFLLAQSMKYNPRLFVYDRDRGMEPFIRSVGGYYKVLQQGMPSGFAPLQIEPTKRNIA
                     LIKNLFRICVETTNNGPISATMATELAEGVDAVMGEGSLIPREARTVTILDGYVNEVV
                     ENGVSLKGLLREWTREGQYGWLFDNDKDSLDLSTNDIFGFDLSEFIAAKEEVSSPART
                     PLMMYLLYRVRDSIDGKRRVIQCFDEFHAYLDDPVIEREVKRGIKTDRKKDAIYVFAT
                     QEPNDALSSRIGRTIMSQTVTKICLRDPEAIREDYAFLTDAEYDALMSITEHSRQFLV
                     KQGQQSAIASFNLYPRNSDDIDADIKTMDNVLSVLSGEPQNAEIAHELVERLGNDPEV
                     WLKEYWRLTA"
     gene            complement(18472..18789)
                     /locus_tag="ACHKNB_RS12915"
                     /old_locus_tag="pgaptmp_002583"
     CDS             complement(18472..18789)
                     /locus_tag="ACHKNB_RS12915"
                     /old_locus_tag="pgaptmp_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000496058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HGU4196073.1"
                     /translation="MFVDGKRPLFKGATRLPRALGVPRNVAMLIFMISASLFMIIHMW
                     AILVFVFLWIPSAALTKYDDRMFRIMGLWLKTKFSNWFDSPFKQWGGSSYSTADYKRK
                     GLK"
     gene            complement(18839..19132)
                     /locus_tag="ACHKNB_RS12920"
                     /old_locus_tag="pgaptmp_002584"
     CDS             complement(18839..19132)
                     /locus_tag="ACHKNB_RS12920"
                     /old_locus_tag="pgaptmp_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HGU4196074.1"
                     /translation="MTTLFKKYGPAVVMGVLSIALPQIALAAGTDTGESTATSLQTWL
                     NTWIPLGCAIAIMVSCFMWMLHVIPASFIPRIVIALIGIGSASYLVSLTGVGS"
     gene            complement(19142..19423)
                     /gene="korA"
                     /locus_tag="ACHKNB_RS12925"
                     /old_locus_tag="pgaptmp_002585"
     CDS             complement(19142..19423)
                     /gene="korA"
                     /locus_tag="ACHKNB_RS12925"
                     /old_locus_tag="pgaptmp_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor KorA"
                     /protein_id="HGU4196075.1"
                     /translation="MERLYLVLQSQILELFEAGKVKEVTIERVSLKKWYPVFQIDDEQ
                     LGQIACSIRVNKEHELRTWADLRLLAEFLKDKCGVEECRLNLKSTEDSE"
     gene            complement(19432..20031)
                     /locus_tag="ACHKNB_RS12930"
                     /old_locus_tag="pgaptmp_002586"
     CDS             complement(19432..20031)
                     /locus_tag="ACHKNB_RS12930"
                     /old_locus_tag="pgaptmp_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGU4196076.1"
                     /translation="MAYIVGHESSNGPYRININGSTQLKQQPRTEAEAVSIAKVLLKD
                     NKSFDMGLAQINSNNLVGLGLSVDDIFKPCINLRASQTILKACYDSAMKSYPAGQVAL
                     RHALSCYNTGSLTNGISNGYVTKVINVARQSNDLKIPTLLPDGQTSEDSTATDPQQAK
                     STAPQYDGEKDVFGSGDGDAFSRNNTDAFLTKQETAKGE"
     gene            20209..20580
                     /locus_tag="ACHKNB_RS12935"
                     /old_locus_tag="pgaptmp_002587"
     CDS             20209..20580
                     /locus_tag="ACHKNB_RS12935"
                     /old_locus_tag="pgaptmp_002587"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HGU4196077.1"
                     /translation="MIRANEIVSRRNEKKTAEGRKVMLVPLKSEKRPKGEPVYRDPDN
                     PFNTWTGIGKRPAWLTAKLDAGISLEAMKMQGVANPREHRPAKYRDPRNAENTWSGTG
                     RRPTWLKELLDSGLSLDDLKI"
     gene            20596..20940
                     /locus_tag="ACHKNB_RS12940"
                     /old_locus_tag="pgaptmp_002588"
     CDS             20596..20940
                     /locus_tag="ACHKNB_RS12940"
                     /old_locus_tag="pgaptmp_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196078.1"
                     /translation="MGEIIDFAERQKSRRKKKASSIPPIFRKFRAHAIRLLASIIKSG
                     SYSIAYIVKKITGKLIKFFTILTIFVFVVEYIAGAIGYKSLYNAALLLMLLTVINILA
                     GVCLNKLLRTKQ"
     gene            20937..21251
                     /locus_tag="ACHKNB_RS12945"
                     /old_locus_tag="pgaptmp_002589"
     CDS             20937..21251
                     /locus_tag="ACHKNB_RS12945"
                     /old_locus_tag="pgaptmp_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HGU4196079.1"
                     /translation="MKKLLIPLIAAGSLLYLPASHAEDPCKVIMCMAGKLTGDSGGSE
                     CNSAEAAFFNIVKKNKHGFLPNHTKDARKAFLNECPDNGEGGSNQSMISQIISKYGKV
                     RL"
     gene            21297..21608
                     /locus_tag="ACHKNB_RS12950"
                     /old_locus_tag="pgaptmp_002590"
     CDS             21297..21608
                     /locus_tag="ACHKNB_RS12950"
                     /old_locus_tag="pgaptmp_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196080.1"
                     /translation="MKKIIFGALACTALLTGCVSQDKGNAMQSQLNNQQRQINELSVR
                     LQSAESRLSKQEGKLRNELLQSSGYCYLNGARYSTGTVLYGRICQNQSGSASWQVYNR
                     R"
     gene            21657..22229
                     /locus_tag="ACHKNB_RS12955"
                     /old_locus_tag="pgaptmp_002591"
     CDS             21657..22229
                     /locus_tag="ACHKNB_RS12955"
                     /old_locus_tag="pgaptmp_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HGU4196081.1"
                     /translation="MNMIPFPTVENLVLWVCGALVLLATLFFRRSVQHRRHKRKQQSA
                     RRVLERIKTLPGFPQKISYLRKIDPFVFEELLLHAFEDAGFEITRNAKYTGDGGIDGR
                     FYVDGRKFLIQAKRYSSWVSTAHIHQLDALAKKDGSLAVFCHTGKTRKQTLDTYKAHV
                     RVLIISGERLISLVEGDTRLANRLRSFAGK"
     gene            22829..23395
                     /locus_tag="ACHKNB_RS12960"
                     /old_locus_tag="pgaptmp_002592"
     CDS             22829..23395
                     /locus_tag="ACHKNB_RS12960"
                     /old_locus_tag="pgaptmp_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4196082.1"
                     /translation="MIIGYARKSTHLQDVAHQVDELTKAGCEQIYQEQTSRGGPKRDK
                     NGAPELENCLKALREGDTLVVWALDRLGGSLGQVITLLDDLKKRGITFIAIKDKIDTS
                     APVIGEIYTHLMAIFSSFERNRNIERTRSGLAAARARGRVGGRKPSLSEEDIKQIKIL
                     LADPEMTVGAVAKRFNVSRMTIYRYTTK"
BASE COUNT         6000 a         6091 c         5834 g         5972 t
ORIGIN      
        1 aataccgcca cccgaaaaat tgccggtatc ggatcactaa ggagtaagca tgaccactgt
       61 taccacccct tcccagctga aaaaagaagt cgaatctcag aaagaattat tgctgcgcgc
      121 ctgcctcgaa gcgttcaacc agctgcctaa tcagcgcctg caaggcgctt tcccatcgac
      181 gtatgcactg gcggcaaaat tagaccagct gctgcaacaa actaaatagc actgacgacc
      241 cggccaagcc gggtctttaa cagaaagatc gaggacaaac gacatgacca cacaaaccct
      301 tgagcagact cttgaagatt ttcgtcgcca gtgtgagagt tttgcgcgcg aacagcagcc
      361 ccgctgcggc ctgatttatg aactgtatca gcgccgcctg agcgcggata ttgacggcta
      421 tcttgctggc gttccggccg agtatcggga agaactgatc gcagtagcgc ggcgtgaatt
      481 tgactatctg acccaagacg agatcgcgga agaaatccgg caggatcggg aaaacggtta
      541 ttgcagccac ggtattgagc gtaattgttg cccgctgggc tgcggagatc tagactatta
      601 ctgagccgcc gaaagaaaaa agcccggatt gaccgggctt ttttctttcg gctgcgcggc
      661 cgttcctgag atagccggtg gttctttgtc tctgtgtaaa tatatattgc acaatacact
      721 acaaatttag ttcgttggct tcgacggcaa gccagcagtt aaaggtttcg cctttccgtt
      781 cacccccgca acctccctcc gctttcagct ttccgcaagc ctggcatttt cccagctgtg
      841 ccttttgctc ctgccactgc tgccagttgc ggatcagcag cagctggaaa aattcgttcg
      901 ttgaataagg cgttcgtcca ggacggcgaa cccggcacag ctcctgcaac attgcttctt
      961 cctgttttgt tatcgtgact gtcacgctag tgccttcttc cttgagccgc gcgcggcggc
     1021 gggcctggcg tatacgggcc agttctcgct gtcggttatt cattattttt ctccccgtga
     1081 tggtcacgcg tgactgtcac gctgaattaa aacgggcatt gcccctgcgg ggccgttctg
     1141 ccgtgctgcg catcgagccg gttcccgtct cgccccgctt acgcgggcgg cggccactaa
     1201 tgcctccggc cttcggcctc cgcgctccgc ttgaaaaaaa accgggacac gcccggtttg
     1261 ctttatctaa gtaggagtat cgctatcgcg gcgcctactg cggcgcccac gatggtgcag
     1321 ccagctgtaa taaaaaggat ctgacgggta aacgtctcag cactggacgc ctgcgcgcgg
     1381 cggaatttag ttagaaattc ctcacgcgcc ttagcatcat cactaattgc caggctaatt
     1441 gaacgctggt tttctactaa agcccgttta agggagtctg ccgtctctcc taaactctcg
     1501 gtgtcttttg ccagcttttc cgaaagctca gaaagtttct gcgtcatttc tttctgagtt
     1561 gcggtgattt ccttcgcgtc acctaaaaac cagtccatca gaacttctaa tttgtccggc
     1621 tgcttttcca ttttattctc ccattaaagc cgcatatgct ttatcgagat tagcttttac
     1681 cggaatcgct tgtttcatgt atgtataacg gcgtgccagt tttttgcgct cgtcagcatc
     1741 ggttgtttgt tttgcacggg atttgaattc ttctggatct tcagcggtca atacttcgaa
     1801 tgagatacgg tgaaacgcca ggtattcgaa aacctcactg ttataaataa cagccttctt
     1861 gcttattttg ccaatcttcg tttctttgac aaactcaaac accggcgcca gcacatcttc
     1921 tacttcactg tctggactaa tacggtttgg aacaaagata attttcttgc tgctcacgcc
     1981 agctttactt aacgtgtgtg ccgttttcag actttcatca atcgccttat tatccggcgt
     2041 taccgggatt acatatttat caaattcgtt cgcgccactg tcaaagcgag acatagccat
     2101 caggaacgct tcaacgtttg acgcgccaat atcaataatc cctgcatctt caaaaacaat
     2161 atcctcaatc aaacgtgaga agtcgtcacc tttaaaactg gtcacatcct caatacccag
     2221 atcggaagca gactggttga tagtctctac cgcatagaac tttgcaccag tcaggcgcgg
     2281 ggctaacagg tacgatgaaa ttaaggtttt accaacactg ccactgtaat taattaccgc
     2341 tactttcata aatcacctca gaattttttg ttgaacttct tagggtcgaa atcctcaccg
     2401 attttattga agaactcttt atcaataatg ccgggactct ctaccttttc actttcctgc
     2461 gcttcaggct cagacggttg tgtattgtgc aatacacttt cctctgtacc cttcgcgcta
     2521 ctcttgcctt gtgtattgtg caatacacta tccgcatcat ccggcttact gtcttgtgta
     2581 ttgtgcaata cacttttccg gttttgccgc gctctataca gtgccatctc aagccctttg
     2641 gccgttaccg aaaagctgta ttcgctgccg aggatttcag caatttcatg ccgggttatt
     2701 cccctgtcca gccagttctc gatttcatcc aggcgcaatc tgacagcagc cgataagttg
     2761 cggggtttca gatcagcagg tttattcata acccctccgt tgtatgaagc tgactctaca
     2821 gcacccgtaa aaaggggtca atgctatttt gtagtttctc tgtagtttct ctgtagtttt
     2881 tgagtagttt atcacggaat actgtagttt ttttgtagtc gattagtagt ctatcatcac
     2941 ctgtataaag cggtttttgt agtctttctg tagtttctcc gtagtttctg gctgctcaga
     3001 tggtcatgca agcatgacca tcaaagaata ggacgctttt tagccagata gtccttaacc
     3061 tacggagatc gctcctacca aaaaagatga ttttttatcc cttaacctgc tatacaccta
     3121 acgcagcgtg ataaatcacg ctatcccact gatttaaaat aaaaaaaacc atatttagcc
     3181 atactgtagt attatcacgg tattacaaaa ataatataac tttctgattt ataacatgtt
     3241 tgatttatta ctttggtaat actatgattc caccgtatta gatacccttt ttttcgttta
     3301 aaaaatgcac aaagtgaggg ctgaatgcca acaataaccg caaaagtatc agatgaactt
     3361 ttggcctata tagaccgggt ttcaggcggt aatcggtcag agtatctgcg gcgctgcctt
     3421 gaagctggcc caggtgatcg ggagtccggg ttgaaaatag ttgccgacca gctgagcgac
     3481 gtaaatcgca aactggatta cctttttgac cgtgcttcag atgcggattt tggctcactg
     3541 cgcgatgagc tgaaggcaat aaccgaaacg ctatctggcg taaaattccc accggcagga
     3601 cagatgatgt tgcatgaatc actcgctatt gaaacgctga tcctcctgcg ttcgatagca
     3661 gaaccgggca aaaccaaagc ggcaaaagca gaggttgaac gtaacggcta caaagtctgg
     3721 gaaccaaaaa aggagcgctg aaatggacga tagagaaaga ggcttagcat ttttatttgc
     3781 aattactttg ccgccagtaa tggtatggtt tctagttgct aaatttacct acggtattga
     3841 cccatcaacg gctaaatacc tgataccgta tcttgttaaa aacacttttt cgctatggcc
     3901 tttatggtca gctttaattg ctggctggtt tattggtatt ggcggtctgg tcgcttttat
     3961 catttatgat aaatcacgtg tgtttaaagg cgaaagattc aaaaagattt atcgtggtac
     4021 agagcttgtt cgcgccagaa cactcgctga taaaacacgc gaaagaggtg tcaaccagtt
     4081 aaccgtggct aatatcccca tacctacata cgctgagaac ttgcattttt cgattgccgg
     4141 gacaaccggt actggtaaaa ccacaatttt caatgaactg ttatttaaga gcattattag
     4201 aggcggcaaa aatattgctt tagatccaaa tggggggttc ttaaaaaatt tctatcgtcc
     4261 cggcgatgtt attttaaacg cctatgataa acgcactgaa ggctgggtgt ttttcaatga
     4321 aatccgccgt tcatatgatt acgagcgctt agtgaactct attgttcagg aaagccctga
     4381 tatggctact gaagaatggt tcggctatgg ccgtcttatt tttagtgaag tttcgaaaaa
     4441 acttcacagc ctatacagca cagtaactat ggaagaagtt attcactggg cctgtaacgt
     4501 tgaccagaaa aaattaaaag aatttttaat agggacgcct gctgaagcta ttttttccgg
     4561 gtctgaaaaa gcagttggaa gcgcgcgatt tgttctcagt aagaatcttg ccccacattt
     4621 aaaaatgccg gaagggaatt tttccctgcg tgactggctt gatgatggaa agccgggaac
     4681 cctgtttatc acctggcagg aagaaatgaa aaggtcactt aatccgctaa tttcctgctg
     4741 gctggattcg attttttcta tcgtcctggg tatgggggaa aaagaaagcc gcattaatgt
     4801 atttattgac gagctggaat cactccagtt tctgccaaac ctcaacgatg cactgaccaa
     4861 agggcgtaaa agcggtctgt gtgtttatgc tggctatcaa acctattctc agctggttaa
     4921 ggtttatggt cgggatatgg ctcagaccat tctggctaac atgcgttcta acatcgtgct
     4981 gggcggcagc cgtctcggtg atgaaacgtt ggatcaaatg tcgcgctcac tcggtgagat
     5041 agaaggcgaa gttgagcgta aagaatccga tcctcagaag ccctggattg tccgtaaacg
     5101 ccgcgacgtt aaagttgttc gtgccgtaac gcctaccgaa atatctatgt tgccaaacct
     5161 caccggctat ctggcgttgc ctggtgatat gcccgtcgct aagttcaagg ctaaacacgt
     5221 taaataccat cgcaaaaacc ctgttcctgg cattgaactg agggaaatct gatgcttgat
     5281 ataaccacga ttacccgcca gaacgtcact agcgttgtgg gctactactc tgatgcaaag
     5341 gatgattact acagtaagga ttcatcattc acgtcctggc agggaaccgg agctgaagcc
     5401 ctcggcttat ccggggacgt tgaatcagcc cggtttaaag agctgcttgt cggtgagatc
     5461 gataccttca cgcatatgca gcgacacgtg ggtgatgcca aaaaagagcg tctgggctac
     5521 gacctgacgt tttcagcgcc taaaggggta tccatgcagg cgctgatcca cggcgacaaa
     5581 accattatcg aggctcatga aaaagctgtt gcagctgccg tgcgtgaagc tgaaaaactc
     5641 gcgcaggccc ggacgactcg ccaggggaaa tcagtaaccc agaataccaa taaccttgtc
     5701 gtcgctactt tccgccacga aacgtccagg gcgctagatc ctgatttgca cacccacgct
     5761 tttgtcatga acatgaccca gcgcgaggac ggccagtggc gcgcgctcaa aaatgatgag
     5821 ctgatgcgta acaaaatgca cctgggcgat gtttacaaac aggagctggc gctggagttg
     5881 accaaagccg gttatgagct gcgttacaac agcaaaaata acacgttcga tatggcccat
     5941 ttttcagacg aacagattcg cgctttttcc cgccggtcgg agcaaattga aaagggactc
     6001 gctgcaatgg gcctgacgcg ggaaaccgcc gatgctcaga cgaaaagccg cgtctcgatg
     6061 gcgacgcgtg aaaagaaaac agagcattcc cgtgaagaaa ttcaccagga atgggcaagt
     6121 cgcgccaaaa cgctcggcat tgattttgat aaccgggaat ggcagggaca cggtaaacct
     6181 ctggaggctg atattgcgcg caatgcggcc ccggatttta ccagcccgga ggttaaagct
     6241 gaccgggcta tccagtttgc agttaagtcg ctgtcagagc gcgatgccag ctttgaacga
     6301 cagaagctga ttcagatcgc taataagcag gtgctgggcc atgccacaat ggccgatgtt
     6361 gaaaaagcat acctgaaggc ggtacagaag ggcgccatca tcgagggtga ggcccggtat
     6421 caatcaacgc tgaaggtcgg tgcttcagtt atggccgaga cactgacgcg taaagagtgg
     6481 atcgactcgc tgacgaatag cgggatgcgc gcggataaag cccggtttgc agtagatgat
     6541 ggtattaaaa acggcaggct taaaaagacc agccaccgcg tcactaccgt ggagggcatt
     6601 cgccttgagc gttccattct gactatcgaa tcacgcggcc gggggcagat gccgcgacag
     6661 ctgaccacag agattgcagg ccagctgctc gccgggaaaa ccctcaaaca ggaacaaatg
     6721 cgcgcggtta cagaaattgt gacaagcaag gatcggtttg tggccgcaca tggttacgcc
     6781 ggtaccggta aaagctatat gacgatggcc gctaaggagc tgctggagtc acaagggctg
     6841 aaggtcacgg cgctggcccc ctatgggacg cagaaaaaag cgcttgaaga tgatggatta
     6901 ccggcccgca ccgttgccgc cttcctcaaa gcaaaggata aaaagctgga tgaaaaatca
     6961 gtcgtattca ttgatgaagc cggggttata cccgcgcggc agatgaaaca gctgatggag
     7021 gttatcgaga aacataatgc ccgcgcggta ttcctggggg atacgtcaca aacgaaagcg
     7081 gtagaagccg gtaagccttt tgagcagctg attaaagcag gtatgcagac caactacatg
     7141 aaagacattc agcgacagaa gaatgaagtt ttgcttgagg cggttaagta tgccgctgaa
     7201 ggtaatgccg cgcgcgccct gaaaaatatc actggcgtga acgaactgaa ggaagaagcg
     7261 ccccgactcg ctcaacttgc cgatcgctac ctgtctttgt cctcagaaca acaggatgcc
     7321 accctgataa tctctggtac gaacgcctca cgcaaaaccc tcaatgacta catccggggc
     7381 aatctggggc ttgccggaac cggcgaaacg ttcacgctac ttgaccgcgt ggattcgaca
     7441 caggcggaac gccgcgacag ccgttatttc agtaaaggcc aaatcattat ccccgaacag
     7501 gattacaaaa atggtatgaa gcgtggcgag tcttaccagg ttcttgatac cgggccgggc
     7561 aacaaattaa cggttgaaag cagcagcggt gagcagatcg ctttcagccc gcgtacacac
     7621 accaagctgt ctgtatacca ggcagtcagc gccgaactgg cgccgggcga taaggtcatg
     7681 gtaacgcgta acgataaaac gctggacgtt gccaacggcg accgcttcac ggtgaaaacc
     7741 gtggaaggtg aaaaactgac gcttgaggac aaaaaagggc gcacggttga gctggacaaa
     7801 agacaggctt cttatctttc ctatgcttat gcaaccaccg tccacaaatc ccaggggctt
     7861 acctgtgatc gcgtgttgtt caacatagat accaaatcgc ttaccacttc taaggacgtt
     7921 ttctatgttg gtatttcgcg tgcgcgtcat gaggtggaaa tttttaccga cgataaaaaa
     7981 tctttggcat cgagcgtgag ccgcgacagc ccgaaaacca cggccgcaga aattgaccgt
     8041 ttctttgggc ttgaggccag gtttaaggat attggccgcg ataccagttt agaaacccga
     8101 tcagctgaaa aaggtctgcc agaagccacg ggggaaagca tggcgtttaa ccagaaaccc
     8161 gatgaacata acatgacaac cggcactgat tatcagccgg tgagcaacgc agaggacgcc
     8221 tttcatctta agcagaaccc tatggatgat tccgttggcc tgcggcgtca tgaaacacag
     8281 caaaatgatg cagagctggc ccatgattat gctgctgccg acgatcagca gtggagcgcg
     8341 caggagtatg ctgattacga gcattatgcc gaagcatcag actacgactt tgacagcagc
     8401 atttacgacg attacgccat gccgcagacg tctcaggcgg aacagagcca taccggaaaa
     8461 gaacacaccc atgagcatga gcatgaagaa gggggccatg aaatctgatg gagcagaccg
     8521 acaagcgcaa gcaggataaa ctgaagttcg accgggtaat taatctggcg cgcagactgc
     8581 cgcaaccggc gatccatgac ttactacgcg cgctgatact gccaattcag gccgattact
     8641 tgctggccgt tggcacggag ggccaggacg cgcgcccgga catgaacgag cgcgagttct
     8701 tcttcacaaa aatcatctgg gcgatggatt acacgcacat gaaatcactc aggcttgctg
     8761 ctgaagattt tcccctggcg cttgcgacgg ccaagatcct gccgtggccg tggggtgaat
     8821 caagctaccg gagtgcttta gccgatatag gcagcgctaa gggcaatccg tgggtacagg
     8881 atattaacca ccgcgttact ttgtggttgc cctggcggat aggctttgta cgaggaggaa
     8941 accattctat tgccagcggc gttctggccg gtgagggtga ggtaatacca gatacggttt
     9001 atgatatgcg gtatttgctc gatattgtca gcactgacgg gtattactgg tacatgagcg
     9061 ggaagatctg cgagagagtc agtgactacc gtacagccgc cttttttgaa ataggtcgcc
     9121 tgcttacact gtgattcctt caaaatatct caacgaaatt tcccgaccac tgtttaagct
     9181 aaattgctga tatttctgtt tttcgatttt tgccggcgcg atgccggcga cgttggccag
     9241 ctgtcccggg cgcgatactg aaatcagttt ctttgaacaa aaaaaaccgc ctttcggcgg
     9301 tcattattca atagctggaa cggtagtctc tcccccgatt ccagcggtct tgccagtgtt
     9361 cgaggaatga ttcagcgagc ttaggcatgt tccagatcac caccgcattc tccgagtttt
     9421 tcgtttcggc cgctttggtg aaattaaagc tgccagtttc aacggtcaca ttatccacga
     9481 tgatcacctt gtcatgctgg ataggaaaat cactgtcagt acgcaaaggg atgccgctgt
     9541 tcgctatgta gttcatggcc gcaatgctgg cacgtcccgt attgcccctt tcatcaacca
     9601 cgatttttac atcaactccc cgttttttgg ctgctaccag tgccttcata atatccgggg
     9661 cggtaaaaga ataagccatc atccgtatag aggtttttgc ggagtcaatg gcgctcagga
     9721 cgagaacgcg ggcgcttcct tcaggcgagt agccaacctg aacggatggt tccaacgcaa
     9781 aggcgggcag ggcggctgtc gtaaatgttg cgaccagaag ccaggttgct aactttttca
     9841 ttacaggctc ccgttcacac actgcatttt atattcaggg tcaaaataaa tttctttgat
     9901 tttccgcttt tccatatgca ccacaacgtc aatggtggag tagagcattc gcataatatc
     9961 gctcatatcg agcatacggc cgataggggt cgccttgata agcagcccaa tacggttaaa
    10021 ggcatcgcgc gcagagttag cgtgcgttga cataacaccg cctggatggc cggtattaag
    10081 tgctttaagg taatcccacg cagcatcatc cctaagctcg gtcatgatga tacggcccgg
    10141 tgtcagacgc atacaggctc gcagggcatc agtggcgctg acgcggccga tctttcctgc
    10201 atcgccgtac atcatataaa cggcctcgat aacgtgatcg accgtgactt cgtgaacgtc
    10261 ctctaaaata attacgcgct cgtctttatg tagcgatttt aacaacgcgc gcgtgagtac
    10321 cgttttcccc gacccggttt caccgcagat cacgatagtg cgtttcttct caacggcggt
    10381 ttgcaggaat gcgggccatt tttcgctgct gtgcagctct ttaagaaaaa aatcatcatc
    10441 cgttaggctt tgcttgctgc cggtaatctt ccggcagtca ctgaaaatcc cctcgctggt
    10501 cagctgctcc agatttttat ctgccgccaa atccttacga aacgctacgg ccgttgtacc
    10561 gtcaatcacc gcagggggca gacagataac gcccctgatc ccgccaggca ggatcacgtc
    10621 attaatggcc tgcatggtca gcttgttgct gctcaccaac gatttagcaa ggttcctaat
    10681 aaaatctgcc gtaatcgccg cgttctgcac aaccctgcgg ccgctgaacg tatcacagat
    10741 aacttcctga aagcggttaa tgcgaatttc aaaaacagta ggatcttcta aatactcgcg
    10801 cagtgggcca agttgataga aagctgcatc agtcatgatt actcctgaag aaaagcgggc
    10861 gctaagcgcc cactttttta gttgtctgcg agcgtataaa cgccgctgaa atcgaggtcg
    10921 cgggcaacaa aaatgctcac cgcatcaccc tgctgatcgt agagggtagg ggggatagac
    10981 atgtaagagc ggagtgcttc agacgccagc tgctcaccgc tgttttctgt gctgttgtac
    11041 tgaatgttat tactctgcgt ctggttaacc agcgccgtta aggtgtcaga gaacaacgaa
    11101 atcatgatcg caccacgcag acgctcccac atatgggtat ccacctggcc cggaatcccc
    11161 gcgctgccga gtgagttcgt tccggcactg tcaatattaa cgattgtccc gtcctggtca
    11221 ttgcggatac gctcccagag aacaaacacg cgcgcctggc cgtctttgat accaccggta
    11281 atctgcccgt caacccatga gcctttatca atcagtctaa cgagtccatc agctgagtaa
    11341 acgtcctgtg aaacccggca ggaaacctga cccggaacag tggtatccag ctcggtgccg
    11401 gtaccacagg ggatcatttt gcctttcgga acagtcaggc tgggattagc catgactcca
    11461 gcgcggctag ccttcagcct tgcaggagtc aggtttttag cgagtgccga actaccttcg
    11521 cttgtttcgt tgtcctgggg ctgcactccg ggactattgc ttgtggccgc ctgattagtt
    11581 tgggccagct cgccgcccag acgacgctgc atagccagtt cttcaggcga aggttcttta
    11641 cgcttattag aggttcgcgc gccggtattg ctgctgcccg catctgcatc tgcctgtgcg
    11701 gcagcctgca cagcacgggc atcagtggcg ctattctgcg cagttgcagg cttattaaca
    11761 tcaggatcgc tgttaaagct gtagtttggc agtgtatttg cctgttgcgc tttaccgccg
    11821 tctttatcag cttcagcttt agccggggcg cgaattttac ccatgaccgt aatcccgatg
    11881 aataccaaag caagcagcgc catcagtatg acaaaggctt tcataccagg agccgaacgg
    11941 cggttactgc ctttaaatcc gccacgctcg ctttcaaatt caccgtctcc ggtgttttca
    12001 tcgagttcct gatctacatc gacactttta cgggccatca gttatcctcc ccaatttgaa
    12061 ccctgcgcac atccggggaa gccgtaccgg ttgctaccgc accggcgccc ggcgcgaaat
    12121 tattattacg aacgccaacg actttatcgc ccagacgaat acgccactct ttagcgacgg
    12181 tttccacctc gatgatgttg cggttctcac ccacaacatg agagttaggc agcgtttctt
    12241 tgccactggc cgagatcatg tagacctgcg gtaactccgc attggccgga aactcaaacc
    12301 gggtaaagcg gtagttatcc cagacgtgaa ccggctggat gctgcgcatt tcaggctgtt
    12361 cgctcattac gtactgataa ttcttcgccc ccgcaaaagc cgtctgcttc agcttctgcg
    12421 taatgcgttt tttatcagcc gcgcttttgg ctttttcctg ctgctcaaac ggatattcat
    12481 aggtcagctg aagaacggcc tggcgcacag cccacggcgt ttcaataaag gattttgata
    12541 ccgtaccgtc tgcatttttc ttcgtttctt caccgatgaa atggaggacg atgttatagg
    12601 tgcgcttatc ggtgacgatc accaggttgg tatcactcat ggcctgtttc ggcttcacaa
    12661 aaaaatggtt cattttgtgc gcaaacgtcc agctttcaga atcgccaaaa gcatgagtga
    12721 tataggtttc gtcaggcgcg acaacaatgt gggtagccac accggcgata gcgtcaattt
    12781 tgaccacgtt aacagggtta taaacaacgc ttttaatgcg gtagtcataa ggagaattgc
    12841 ggccaacctc aagcgccata acgttagtgg ccgcgcctcc caggactgac aaaacgactg
    12901 ctgaaagaag tagttttttc atggggcaga cctcagttaa cttccgggtt gacgcgataa
    12961 ctcgtcacgc ggaaacccag cgggttgaca taacgctgct cagcattcat cgccagcgat
    13021 ttatattcat accccataat ggcaatccag cgctgcggct gatcatcaac gggattgctg
    13081 cgcacgcggc gaaccgtagt aaagcgtatc gttgctacgc cgtgcggttt atcgaggatc
    13141 acagagttaa tcttcacacg ggtcgtttca ctgtcgccca gaaccttatc aagaccgttg
    13201 cggcccttga acttgctctg gtaagactct gccacgttcg gcgtggacat taagccgacg
    13261 gccgtataat cgacctgaac tgaatagaag tcatagctct cacggtgaat gacatattgt
    13321 gtcagccaga acttatcaat ttcgtcacca taagaggtct ggtcgcgggt cagcttgacc
    13381 tgctgtactt cgtgagtggc ctcgttgagc gttagcagat gtgcggggat tggctggctg
    13441 tacttatgca ccacgtaacc aactaaagag agtgcaaaaa cagttaccac cgctgaaccg
    13501 gtggcaacca tccaggcggt acgccgggac ttcagcactt catccatcag atcaacttca
    13561 agccctttac ggctttcgtt gaactcttta atggcttcac gtgtaagccc tgttttttta
    13621 ttagctctca tttgcaccac cttgcgtatc aaccgggatt gttttgttta ctggaacggt
    13681 gttgctccag tccggctccg gtggcggttt atgcccgctg gaacaggcgc taatcagaag
    13741 aactcccatg agtaataagc tgcgcattac ggcatccatt atcggttgtt gaacgtacta
    13801 attataataa gtcgtcgaaa ttgcaatttc aacgactttt gttttaattt tatgcagcct
    13861 tcttcccgcg actgcccctt gccttaccac ttaaattaga accactgttt cctccgctgt
    13921 tgctgccact tcctccacct gccgattgct ggccgccgcc actgttaccg cctctgaacg
    13981 cattaccgcc aaacatgccg tggctgccca tattgccaag cgcctgcatg gaagaacccg
    14041 tagagcgtgc ggcgtcagca actccggcgc tgataccgct accccagctg gcggcaattt
    14101 gcgggatctg gaacagaacg aaaacggaaa tgaccgtcag taataaggcg gcgatagaac
    14161 cagtgattga tgagtaagcg gcatcagagt tcatcgagga caggaggttg tcgaacatct
    14221 gcatgatgaa accaaacacg agcgcaagaa tgacgacgac aaggccatag ttaatgaccg
    14281 acgccagcca gcgagcaaag atgttttttg tcgctcccca cagcaggcag aagattgcaa
    14341 tcgggccaaa acaaagcgta acggccagaa ggattttagc catgatcaca aagcccgcac
    14401 cgaggccgcc cagcaccacg gtagcaatca tcataatgcc gccaatggca tacgcggcca
    14461 ggccgctcga tgaaaacacg tctgcggctt cccatgcggt gttgacgatc ttgatacctt
    14521 tttcaatacc gctatcaata atcgccggta cgccacttgc accgacttta ttaggggcag
    14581 acagtatccc ggcaaaatca tccggcaggt gaagcgccac gttgaccagt tcctgttgat
    14641 accagccgcc cgccgttgca aagctcagga taagggctat ggaaagatac tctttaatca
    14701 gcgaactcag gctgtcgccc gcccccggat taaacgctga gtacatcccc tgcaccatca
    14761 gcttgatggt cagacaggtg gcaatcagag gcgttacatc tgagataatg gtggcaacat
    14821 tggcgctcac cattgacgta atcgccccgt ctacttttgc gaaaatgtct gcgactaggg
    14881 tgaatgccat attgcctcct tactcctaaa ttttcggtga attgatttcg ccgtcttttt
    14941 tgccgaacac aaacagctga cgatcggctt cgcgcgcgtt tttgcaatca ggtttctgaa
    15001 gttcatccgc gtctttcttg cattcctgaa ttgttgcctt gcgctctttc tcatgttttt
    15061 tgtaccactc cacgtcatgc gaggcatcgc aggccaccag gaggaaaggg ataacaagca
    15121 gtagtttttt cataattacc tcagttaatt gaaggtgacg cggtaacgtc cccgccggtt
    15181 ccaaaaacaa aattgtgggt ggcacgttct ttctgcgcac gtaatagctt gtcctgagac
    15241 tgctgcaaca tgttcatcag attcagcttc gcctgctcac cctgaatagc accctgtgac
    15301 gtctggatac gggcctgtaa gtcagcaatc gatttcaggt ctggcgtcgt tttaatctgc
    15361 tccgtcagct cctgcatatc ggtcagttcc tgcatctggt tgttgtaggc tttttctgcc
    15421 ataacgcggt cataagcgcc tttttcagcc agctttttgt tcatgtaggt aattgcttcg
    15481 ctgggcgtca tgtcgtcaac ttccgcatta aactggccca tcatgctgtt aactgaaggc
    15541 gtgacggaag aactggagtt catggcgtcg ctgtagatct ccttccagtt gtccggcaaa
    15601 ttatttgcca gcgtgctggt agacgtcccc agcagatcgc ccaggtcggt tgtctttgcc
    15661 atcgactcat acatattttt ctgcgtttgc agctggcttt taagctgttc cagctgctgc
    15721 gccatttgct gaagctgctc gacctgttta gccagctcag tagggttggt cacgatgatg
    15781 cctgcggaag cgctttgcgc gccccccaga atcaggccgg tggtcagcag tactgccgtc
    15841 agtgtttttt tcatggtgtt ttgcctcatt gttaagccgt caggcgccag tattctttga
    15901 gccatacttc agggtcatta ccgagccgtt caaccagctc atgcgcaatt tcggcgtttt
    15961 gtggttcacc ggacaacacg ctaagaacgt tgtccattgt cttaatatct gcatcaatat
    16021 cgtcgctgtt gcgtgggtag agattgaaag aagcaatcgc agactgttgc ccttgtttaa
    16081 ccaggaactg tctggagtgt tcggtaatcg acatcagcgc gtcgtattca gcatcagtaa
    16141 ggaaggcata atcctctcgg atagcttccg gatcgcgcag gcagattttc gtgacggtct
    16201 gcgacatgat cgtgcggcca atacggctgg acagcgcatc gttcggctcc tgcgtggcaa
    16261 acacatagat agcgtctttc ttacggtcag ttttgatacc acgcttaact tcacgctcga
    16321 taaccggatc gtcaaggtag gcgtggaact cgtcaaagca ctgaatgacg cggcgtttgc
    16381 cgtcgatgga gtcacgtacc cggtacagaa ggtacatcat gagcggagta cgggccgggc
    16441 tggatacttc ctctttggct gcgataaact cggacaaatc gaagccaaaa atatcattcg
    16501 tgctgagatc caggctgtct ttatcattgt caaaaagcca gccatactga ccttcgcgcg
    16561 tccattcgcg cagcagccct ttcagtgata cgccattttc cacaacttca ttcacgtacc
    16621 cgtccaggat agtaacggtg cgcgcctcgc gtggaataag tgagccttcc cccataaccg
    16681 catcaacgcc ttcagccagt tcggtagcca tcgttgcgct gataggcccg ttattggtgg
    16741 tttccacaca aatgcggaac aggtttttaa tgagggcaat attgcgtttg gtcggctcaa
    16801 tctgaagcgg ggcaaacccg gacggcatac cctgttgcag aactttatag tagccaccaa
    16861 cgcttcgaat gaacggctcc ataccgcggt cacggtcata aacaaaaagc cgtgggttgt
    16921 acttcattga ctgcgccagc aggaagttaa gcagtgtggt tttaccttcc cccgacatac
    16981 ccgttattaa cgcgtggccc agcgggcgtt taccgtaaga aagttcttca agcggggtca
    17041 catggaaatt aaaatagagt ggcgtaccgc tgatcgtgcg gaacatggtc agcgctggcc
    17101 cccacgggtt attgtcaggc ttaccacgca taaaattgtg gaacgagctg aagtgcagga
    17161 agttccatga gtttaccgga accgggcgcg gcgcccattt ctggttgcca ggcagtttcg
    17221 cataatatgc agcctcagag gccaggctga tagtaccgcc aaccacgcca cagcctgtca
    17281 gcataacctt cacacgacgc gctttgcgct gtacagcgtt ttggtcatta tcccagacat
    17341 gcacagttcc atgatggtag cccatcacga actctctgga cgtcagcata tcaagcgcgg
    17401 taccaagctg tgccagctgg cttagcgcac ggtcgcgcgt ttcctgcaaa gatttttcct
    17461 gatgcgtcag aaacgtttta gctgaagatt cagagaggca agaaaaactc tgcgtcagaa
    17521 ggtattcaaa atcggcttct ttaagcatat tgagctggcc tggctctgta tcttcttcgt
    17581 agtcacgaaa ttcaatgccg gtggtgtaga agttgtgatc taccgttctg atctggacaa
    17641 catcacccca cagggagctt acagggcggt tgtccatgat gtaatcacgg atacgctcac
    17701 ggcaaacagg cacgatggcc cattccatat ttgcgaggaa ataaaggaac tccagcgctt
    17761 ttgaataagc gtgagcctgc gaaggctcag gctcgttacg ttcgataaca acagcttcgt
    17821 caaaaacgtc tgattcatcg acctcagcca gttcttcacg ctctttttta tcaggcgccg
    17881 gaatttcaac accgcgtttg tcacgataat agatacccag ctgctgaata ccatacggct
    17941 tcattgcttc caggatttgt tcagaaacat cttccagacc ttcaagcgcc tcattctgca
    18001 tttgctgaat ttggtcacga gtcggctttt caaatttcgc cagaaacttc tgtgtttgat
    18061 cccctacctg tttatagata acggtcagat aaaggtcatt aataagctgt ttggaatcgc
    18121 cgtgcagctt acggttatat tggtcaacat aagcagggaa aaactggtca tactcaccat
    18181 ccgggtactc tttagcctca tggtgatatt catgcgtcca cagctctacc tggtctgttc
    18241 cgaagctctt aaccagcgta ttaaggtctt tatgccaagt gaccagttcc cgatctgatg
    18301 cgcagtcatg tgtgcggcca tccagcttga aaaaagccat cagatcgccg ttttccatcg
    18361 aaatcacgta atcattgagg tgataagaat agggcaaata ctttttattt accgatggtt
    18421 ccttacggaa ggcatcaatt ttttttggct tcgtagcggt ggcagctctc attattttaa
    18481 acccttacgt ttgtagtcag cagtggaata agacgatcct ccccactgct taaacggaga
    18541 atcaaaccaa ttactgaatt tggttttcaa ccacaggccc ataattctaa acatgcggtc
    18601 gtcatatttt gttaatgcag ctgaaggaat ccacaaaaag acgaacacca ggatcgccca
    18661 catatgaata atcataaaga gcgaagcaga gatcatgaat atcaacatag ctacattacg
    18721 tggtacaccc agcgcgcgag gtaagcgagt cgcacctttg aaaagtggtc ttttcccgtc
    18781 aacgaacatc tttgtagtcc tcactttagg ggacaaaagt cccccatttt cgcgttggtc
    18841 agcttcctac acccgtcagg gaaaccagat atgatgcaga accaatacca atcagtgcga
    18901 ttacgatacg aggaataaag ctggctggga ttacgtgaag catccacata aagcaactaa
    18961 ccatgatcgc aatagcacaa ccaagtggaa tccatgtgtt cagccacgtc tggagtgatg
    19021 tagcggttga ttcaccagta tcggtgccag cggccagcgc gatttgcggc agggcaattg
    19081 acaaaacgcc cataactacc gcagggccat acttcttaaa caacgtggtc atactttctc
    19141 cttactcact atcttctgtt gatttcagat ttaaccggca ttcttcaacg ccacacttat
    19201 ctttcaaaaa ctctgccagt agccttaaat cagcccaggt tcgtagctca tgctctttgt
    19261 taacccgaat ggaacatgcg atctggccca gctgttcatc gtctatctga aaaacgggat
    19321 accacttctt taatgaaacc cgttctatcg ttacctcctt cacttttcct gcttcaaata
    19381 gctccaggat ctgtgattgc aatacaaggt acaaacgttc catccataac ctcactcccc
    19441 cttcgctgtt tcctgtttgg ttaaaaaggc atccgtatta tttcggctga aggcatcgcc
    19501 atcacccgaa ccaaaaacat ctttttcacc gtcatactgc ggcgccgtac tttttgcctg
    19561 ctgaggatca gtcgcggtgc tgtcctcact ggtctggccg tcaggtagca gcgtagggat
    19621 tttcaaatca tttgattgac gcgccacgtt gataactttc gtgacgtacc cgttagaaat
    19681 cccgtttgtg agtgagccgg tgttgtagca ggaaagcgcg tgtctcagcg caacctgccc
    19741 ggctgggtag gatttcatgg cgctatcata acaggcttta aggatggtct ggctcgcccg
    19801 caggttgatg cagggcttga aaatatcgtc aaccgaaaga cccaggccca ctaaattatt
    19861 tgagttaatt tgtgcaagac ccatatcaaa acttttatta tccttcagca gaactttcgc
    19921 aatgctgacg gcctcagctt cagtacgcgg ttgctgtttt aactgggtac taccgttaat
    19981 gttgatcctg tacggcccat ttgaggactc atggccgacg atgtacgcca ttgttaaggg
    20041 tgaaacatca ggcgcacatc tctgcgcaag cctggcaact tcatcggaag caggcgcagc
    20101 actggcacgg ccagcacaag caaggcaggc cagagcgaga accaggagtt ttggatgttt
    20161 actcatttac gtttcaccgc gtaattgtaa tttcgacgac ttttgtttat gataagggct
    20221 aatgagatcg tgtcaagaag aaacgaaaag aaaaccgctg aaggaaggaa ggtgatgctg
    20281 gttcccctga aatcagaaaa acgacccaaa ggcgaacccg tgtatcgtga cccggataac
    20341 ccttttaata cgtggactgg tatagggaag cgcccggcct ggctaactgc aaaattggac
    20401 gctggcatta gcctggaagc catgaaaatg cagggcgtag ccaaccccag agaacataga
    20461 ccagcaaaat accgcgaccc taggaacgca gaaaatacct ggtccgggac tggccgccga
    20521 ccaacatggc tcaaagagct gcttgatagt ggtttatcac ttgatgatct gaagatataa
    20581 ccggagggta taaaaatggg cgaaattatt gattttgcgg aaaggcaaaa aagcagaagg
    20641 aagaaaaagg catcttccat tccgcccatt tttagaaaat ttcgtgccca tgcgatcagg
    20701 ttgctcgcca gcatcatcaa atccggctct tattcaattg cttatatcgt taagaagatt
    20761 acaggaaagt taatcaagtt tttcacaatt ttaacgattt tcgtttttgt tgtcgaatat
    20821 atcgcaggcg ctataggtta caaatctctc tataacgctg cgttattatt aatgttactt
    20881 accgttatca acattctggc gggtgtatgt ctgaacaaac tgttaaggac aaaacaatga
    20941 agaaactctt aatccctctg atagcagctg gtagtctgct ttatcttcct gccagccatg
    21001 ctgaagatcc ctgcaaagtt attatgtgca tggcgggcaa gctcaccggc gatagcggcg
    21061 gaagcgagtg taacagtgct gaagctgctt tcttcaatat cgttaaaaag aacaagcacg
    21121 gctttttacc caaccacacg aaggatgcca ggaaggcttt tcttaatgaa tgcccggata
    21181 atggtgaagg tggaagcaac cagtcaatga ttagtcagat aatcagcaaa tacggaaaag
    21241 ttcgtttata agcgaacaga atttaattag gaataatctc aatataagga gccagcgtga
    21301 aaaaaattat atttggtgca ttagcctgta cagccctttt aaccggctgc gtcagccagg
    21361 ataagggtaa tgctatgcaa agtcagctga acaaccaaca acgccagatt aacgaattat
    21421 ccgttcgttt gcagtctgcg gagtcccggc tgtcaaagca ggaaggaaag ctgcgcaacg
    21481 aactgctgca atccagcggc tattgctatc tgaacggcgc ccgctattcg acgggaaccg
    21541 tgctttacgg gcgcatttgc caaaatcagt caggcagcgc ttcatggcag gtttacaacc
    21601 gtcgctaatc actagggcgg ttaaaccgcc cttttcttca ccctcagaaa accactatga
    21661 acatgattcc cttccctact gtagaaaacc ttgttctctg ggtttgcggc gcccttgtgc
    21721 tgctcgctac tttattcttc aggcgatcag tacagcaccg gcgccacaaa cggaaacagc
    21781 aaagcgcgcg gcgggtgttg gaacgtataa agacattgcc gggcttccct caaaaaatta
    21841 gctacctgcg gaaaattgac ccctttgtat tcgaagaact gctgttacat gcttttgaag
    21901 atgccgggtt tgagatcacc agaaatgcaa aatacaccgg cgatggcggc attgatggtc
    21961 ggttctacgt tgacggccgc aaatttctca ttcaggccaa acgttattca tcctgggttt
    22021 cgacggcaca cattcaccag ctggatgcac tggcgaaaaa agacggcagc cttgctgttt
    22081 tctgtcacac tggcaaaacc agaaaacaga cgctggatac ttataaagcg catgttcggg
    22141 tgttgattat aagcggtgaa aggttgataa gcctggtcga aggcgatacc aggctggcga
    22201 accggctccg gtcattcgcg gggaagtgat ttcttgcgcg tcttaaattc ttcagcgaac
    22261 ttcatagccg cagtaacttc gaaggtaaga gaatttgggc ctttataacg gacgttcttt
    22321 atacctcgct gactaggtgg cagctcaagc ccttctagca tattatcgct ttttatagcc
    22381 cagactatct cgccgggtga gacgtttatt aactcggcca gtacatcaac gttaatcctc
    22441 atataaccac ctaattatac agaacttacc agcgccgcaa tgcggctcgg tcaacccctc
    22501 acggccgcaa aacaagtttt gcagccgtga ggggttaaat ttattttagc tgctcccagc
    22561 ggatcttact tcactcagtc ttgcagcact aggcccgtca ctcggcaggc cagcgaggca
    22621 cagcgcctcc caaatgttga tattttgatt gtcactcaaa cgatggtttt tgtgacaccc
    22681 cacaaaagga gttgatgatc cacgaaaaca cctttacagg tatcacaaaa cccgtcacag
    22741 tatcaatgta acaaaaatga tgtaacaaat taggcattta agtgatttag caacatcgta
    22801 tgtattttat taaggatgca gaacgtaaat gataatcggc tatgcacgta aatcaacgca
    22861 tcttcaggat gtagcgcacc aggttgacga actgacaaaa gcaggttgcg agcaaattta
    22921 tcaggaacag acctcgcgcg gcggccctaa acgcgacaaa aatggggcgc cggaactgga
    22981 gaactgcctg aaggcgttgc gcgaaggtga tactctggtt gtttgggcac ttgatcggct
    23041 cggcggttca ttgggccagg taatcacttt gctggacgac ctcaaaaagc gcgggataac
    23101 gttcatagcc atcaaagata aaatagatac gagcgctccg gttatagggg aaatctatac
    23161 tcatttaatg gcgatattct ccagctttga acgcaaccgc aacattgaac gaacccgatc
    23221 tggcctcgca gctgctcgcg cgcggggccg tgtaggggga aggaaaccgt cactttcgga
    23281 agaggatatt aagcaaataa aaattctgct tgctgatcct gaaatgacag taggagcagt
    23341 tgcgaagcgc ttcaatgttt ctcgcatgac tatttatcgc tacacgacta aataaattac
    23401 cgccgcaagg cggttttttt ctgttttatg cctggcaggg catggaaggc gaccgcgcca
    23461 gacttcaccg gacgccgggg ccaaatcgcc ggaaactgcg gcgctgaccg gagcaacagg
    23521 ccaaccccct ccctgctaag ccataacccg ctcacagcca cgcagctgcc gcacgtcccc
    23581 cacgggggtg cgcagtgggc gccgcgcgcc tgcgggtcgc ggcgccgtac tgcgagttac
    23641 cggacgctat gcggccggtt acgggggaca ccgcaccgtc acggccagcg acccgctgag
    23701 ctgcacaatc cacggataat acagacgata tatgggtagt tatggaaacc cgcccacctt
    23761 gccggacgac gctgccgcgt gggaatccgc ccaccttgcc gggcgccgct gccgcgtggg
    23821 aatccgccca ccttgccggg cgccgctgcc gcgtgggaat ccgcccacct taccgggcgc
    23881 cgctgccgcg tgggagt
//
LOCUS       DAZFVO020000071        23882 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFVO020000071 DAZFVO020000000
VERSION     DAZFVO020000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.071"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..334
                     /locus_tag="ACHKNB_RS12965"
                     /old_locus_tag="pgaptmp_002593"
     CDS             <1..334
                     /locus_tag="ACHKNB_RS12965"
                     /old_locus_tag="pgaptmp_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4196083.1"
                     /translation="REKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEA
                     KWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEF
                     PQPVSLAG"
     gene            complement(377..1528)
                     /gene="ydiO"
                     /locus_tag="ACHKNB_RS12970"
                     /old_locus_tag="pgaptmp_002594"
     CDS             complement(377..1528)
                     /gene="ydiO"
                     /locus_tag="ACHKNB_RS12970"
                     /old_locus_tag="pgaptmp_002594"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HGU4196084.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1542..3137)
                     /gene="ydiF"
                     /locus_tag="ACHKNB_RS12975"
                     /old_locus_tag="pgaptmp_002595"
     CDS             complement(1542..3137)
                     /gene="ydiF"
                     /locus_tag="ACHKNB_RS12975"
                     /old_locus_tag="pgaptmp_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HGU4196085.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3281..4039)
                     /gene="aroD"
                     /locus_tag="ACHKNB_RS12980"
                     /old_locus_tag="pgaptmp_002596"
     CDS             complement(3281..4039)
                     /gene="aroD"
                     /locus_tag="ACHKNB_RS12980"
                     /old_locus_tag="pgaptmp_002596"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGU4196086.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4070..4936)
                     /gene="ydiB"
                     /locus_tag="ACHKNB_RS12985"
                     /old_locus_tag="pgaptmp_002597"
     CDS             complement(4070..4936)
                     /gene="ydiB"
                     /locus_tag="ACHKNB_RS12985"
                     /old_locus_tag="pgaptmp_002597"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HGU4196087.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4948..6213)
                     /gene="ydiN"
                     /locus_tag="ACHKNB_RS12990"
                     /old_locus_tag="pgaptmp_002598"
     CDS             complement(4948..6213)
                     /gene="ydiN"
                     /locus_tag="ACHKNB_RS12990"
                     /old_locus_tag="pgaptmp_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4196088.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6440..7654)
                     /gene="ydiM"
                     /locus_tag="ACHKNB_RS12995"
                     /old_locus_tag="pgaptmp_002599"
     CDS             complement(6440..7654)
                     /gene="ydiM"
                     /locus_tag="ACHKNB_RS12995"
                     /old_locus_tag="pgaptmp_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4196089.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7754..8110)
                     /gene="ydiL"
                     /locus_tag="ACHKNB_RS13000"
                     /old_locus_tag="pgaptmp_002600"
     CDS             complement(7754..8110)
                     /gene="ydiL"
                     /locus_tag="ACHKNB_RS13000"
                     /old_locus_tag="pgaptmp_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HGU4196090.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8246..8353)
                     /gene="rprA"
                     /locus_tag="ACHKNB_RS13005"
                     /old_locus_tag="pgaptmp_002601"
     ncRNA           complement(8246..8353)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKNB_RS13005"
                     /old_locus_tag="pgaptmp_002601"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8539..9651)
                     /gene="ydiK"
                     /locus_tag="ACHKNB_RS13010"
                     /old_locus_tag="pgaptmp_002602"
     CDS             complement(8539..9651)
                     /gene="ydiK"
                     /locus_tag="ACHKNB_RS13010"
                     /old_locus_tag="pgaptmp_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HGU4196091.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10040..13096
                     /gene="ydiJ"
                     /locus_tag="ACHKNB_RS13015"
                     /old_locus_tag="pgaptmp_002603"
     CDS             10040..13096
                     /gene="ydiJ"
                     /locus_tag="ACHKNB_RS13015"
                     /old_locus_tag="pgaptmp_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HGU4196092.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13093..13503
                     /gene="menI"
                     /locus_tag="ACHKNB_RS13020"
                     /old_locus_tag="pgaptmp_002604"
     CDS             13093..13503
                     /gene="menI"
                     /locus_tag="ACHKNB_RS13020"
                     /old_locus_tag="pgaptmp_002604"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HGU4196093.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13603..13791
                     /gene="ydiH"
                     /locus_tag="ACHKNB_RS13025"
                     /old_locus_tag="pgaptmp_002605"
     CDS             13603..13791
                     /gene="ydiH"
                     /locus_tag="ACHKNB_RS13025"
                     /old_locus_tag="pgaptmp_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HGU4196094.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14339..14707
                     /gene="sufA"
                     /locus_tag="ACHKNB_RS13030"
                     /old_locus_tag="pgaptmp_002606"
     CDS             14339..14707
                     /gene="sufA"
                     /locus_tag="ACHKNB_RS13030"
                     /old_locus_tag="pgaptmp_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HGU4196095.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14716..16203
                     /gene="sufB"
                     /locus_tag="ACHKNB_RS13035"
                     /old_locus_tag="pgaptmp_002607"
     CDS             14716..16203
                     /gene="sufB"
                     /locus_tag="ACHKNB_RS13035"
                     /old_locus_tag="pgaptmp_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGU4196096.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16213..16959
                     /gene="sufC"
                     /locus_tag="ACHKNB_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /pseudo
     CDS             16213..16959
                     /gene="sufC"
                     /locus_tag="ACHKNB_RS13040"
                     /old_locus_tag="pgaptmp_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
     gene            16934..18205
                     /gene="sufD"
                     /locus_tag="ACHKNB_RS13045"
                     /old_locus_tag="pgaptmp_002609"
     CDS             16934..18205
                     /gene="sufD"
                     /locus_tag="ACHKNB_RS13045"
                     /old_locus_tag="pgaptmp_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGU4196097.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18202..19422
                     /gene="sufS"
                     /locus_tag="ACHKNB_RS13050"
                     /old_locus_tag="pgaptmp_002610"
     CDS             18202..19422
                     /gene="sufS"
                     /locus_tag="ACHKNB_RS13050"
                     /old_locus_tag="pgaptmp_002610"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HGU4196098.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19435..19851
                     /gene="sufE"
                     /locus_tag="ACHKNB_RS13055"
                     /old_locus_tag="pgaptmp_002611"
     CDS             19435..19851
                     /gene="sufE"
                     /locus_tag="ACHKNB_RS13055"
                     /old_locus_tag="pgaptmp_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HGU4196099.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20000..21004
                     /gene="ldtE"
                     /locus_tag="ACHKNB_RS13060"
                     /old_locus_tag="pgaptmp_002612"
     CDS             20000..21004
                     /gene="ldtE"
                     /locus_tag="ACHKNB_RS13060"
                     /old_locus_tag="pgaptmp_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HGU4196100.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21068..21304)
                     /gene="lpp"
                     /locus_tag="ACHKNB_RS13065"
                     /old_locus_tag="pgaptmp_002613"
     CDS             complement(21068..21304)
                     /gene="lpp"
                     /locus_tag="ACHKNB_RS13065"
                     /old_locus_tag="pgaptmp_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HGU4196101.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(21615..23027)
                     /gene="pykF"
                     /locus_tag="ACHKNB_RS13070"
                     /old_locus_tag="pgaptmp_002614"
     CDS             complement(21615..23027)
                     /gene="pykF"
                     /locus_tag="ACHKNB_RS13070"
                     /old_locus_tag="pgaptmp_002614"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HGU4196102.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(23035..23229)
                     /locus_tag="ACHKNB_RS13075"
                     /old_locus_tag="pgaptmp_002615"
     CDS             complement(23035..23229)
                     /locus_tag="ACHKNB_RS13075"
                     /old_locus_tag="pgaptmp_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196103.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            23584..23793
                     /gene="fumD"
                     /locus_tag="ACHKNB_RS13080"
                     /old_locus_tag="pgaptmp_002616"
     CDS             23584..23793
                     /gene="fumD"
                     /locus_tag="ACHKNB_RS13080"
                     /old_locus_tag="pgaptmp_002616"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HGU4196104.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         6227 a         5755 c         6010 g         5890 t
ORIGIN      
        1 tcgcgaaaaa atcactttag aacagttatc gaaaaaattt cgtgccagcg tcagttatat
       61 ttgccatgaa tttaccaaag agtatcgtat ttcccctatt aactatgtta ttcaacggcg
      121 tatgacggaa gcgaaatggt cactcactaa tactgaatta tcacaggcag agatctcctg
      181 gcgtgtgggt tatgaaaatg tcgatcactt tgccaaactg tttttgcgcc atgtcggttg
      241 ttcgcccagc gattaccgca ggcaatttaa aaactgtttt gcggaacaag aaatcctatc
      301 tgaatttcct caaccggtaa gtcttgccgg gtaaatctgg cggcgcagtt tgttaagaag
      361 ctgcgccgcc tgcagattat ttgttctgat aatctttcag gatctgccga cccgcaacgt
      421 aaatcataat ttcgtctgta ccgccgccga tacgttcaca acggacatca cgccagaagc
      481 gggagacgcg cgcctcatcg gtatagccca gaccgcccat gatttgaatc gcatcatcaa
      541 tgacttccat tgcggtacgt gcacagtaca gttttgccag cgccgcgctg gtgcgcagtg
      601 actgatgctg atcggcttgc catgccactt tcagcaccat gttgcgcatg ttgtcgatct
      661 taatcgccat cagcgccagt ttttcctgga tcatctggtt atgaccaatg ggcttaccaa
      721 aagcgatacg ttggttggca tagcgggcgg catcttcaaa ggcgcattcg gcaaagccgg
      781 tgctgcgcgc ggcgttgatc aggcgctcca tctcaaagtt gtacatcaca ttgaggaaac
      841 ccattccttc ttcgcccacc atgtcgctct cttcaacctc cacgttgtcg agatagactt
      901 cgcaggtgct gagcatatgc cagccgattt tatgcagcgg gttaatctta atgccgggct
      961 tactggagtc gacccaccac agggtgaagg cttttttggg atctttcggt tgcggatcgc
     1021 gcgccaacac cagcatatac gggtactctt tcgcaccggt aataaaggtt ttttgtccgt
     1081 tgatgtagac cttgccgttt ttacgcgtgt aagtggtagt ggcactgttg ttatctgagc
     1141 cagcacctgg ttccgtcaac gccagggcat aggcgggatc acccgtttcc agggtgcttt
     1201 ctgccgtttt acgtagctgc tctgcagaac cgaaacggcg catactgtga atacattgac
     1261 cgttggtgat caaaaatgcc ggagcaccgc attttgacac ttccatcagc gccagcattt
     1321 gggtgacgta atccgcaggg ataccaccaa attcttccgg cacgccaagc atggaaatac
     1381 cgttatccgc cagcgcccgc ataaactcac gcggatatgt cccgttttga tcgcaggtgc
     1441 ggaaatactc ttccggaaag ttagtcgtaa tcagttcgcg aatactggcc agcagcagtt
     1501 cttgttcttc agttaaagaa aaatccatca ttatactcct gttaatgagc cgcttcaggc
     1561 aggacaaaac ccatcgccgc atcgataaat aatctttcgt ccatcagttt gagttctggc
     1621 gaaatcactg gggtgaagtc cattttgtcg agaatatctt tttgtaaatc gacgccaggg
     1681 gcgatttcga ttaaatgcag gccgtcttct ttcagcgtga atacagcacg ttcggtgatg
     1741 taacgaacat ccagttcccg ttcgagcgcg atttttccgc taaaagtaat ttccggtagt
     1801 tcccgaataa atttatttac ccgtccttcc tggacgatat gcaatttgcc atcggcaatt
     1861 tctgttttta aactgcccgc agttaacgtg ccgcaaaaaa taattttctt cgaggtggca
     1921 ctgatatcaa taaatccacc ggtacccatg attttgccat tgaatttatg cacgccgacg
     1981 ttaccgtgct ggtcgacttc agcaaaactc aaataacaaa catccagacc accaccgtga
     2041 taaaaatcaa actgggacgt catatccaga atggcgcggg tattcacgtt cgcgccaaag
     2101 gcgataccct gtgaagtaat tccgccaatc ggacctgttt ctaccgtcag aataaagtca
     2161 tcagcacaac cttcttcccg ggcgactagg ccaataccgt cagcaatacc gacgccgaca
     2221 ttccccaccg cgcctttacg catttcgaat aatgcacgcc tcgcaactaa tttacgttgg
     2281 tttaggggca ggctaagttt ggtgctgtca tcaagggtga agtcaccaga aataaagcgg
     2341 ttaaccggcg caccgccata cagttggctt tgatccggat cgaccaccac aatatccacc
     2401 agataacccg gaatacggac agatttagga tgcagcgtgg ctttcttaac cattttctgc
     2461 acctgcatca tcacaatacc gccgttattg tgcaccgcct gggcaataac caatgcgtcg
     2521 agatacatca cctcatcttc aaaagtggcg tagccttcac tgtcgcaggt ggtagcgcga
     2581 atgaaggcga tatctggcgc aatcgctttg taatagagat attctttgtt atcaaactcg
     2641 accagtttaa tcaggtcttc tttcgtgact tcattcagtt tgccgccttg ctggcgtgga
     2701 tcgacaaatg tcccgatgcc aatatcacta ataataccag gctggtgggc tgcggcggcg
     2761 cgtaaggttt gtgtaagtac accttgtgga tagttataag caataatttt attttgttct
     2821 gcgagttcag aaatacgcgg cgattgtccc cagtgaccac ataatgccca tttcaccaga
     2881 ccttcttgcg ccagaggact aataccacgg tcggcgcgat cgccaagccc tgtcggacta
     2941 ataatcgata aattacgtgg cgtttgagtc tgtttatatt tatcagcaag agctgtaatt
     3001 aacgtggtgg cttccagaat accgccgcca gcgcctaaca cacaaagtgt tgcttcgtcg
     3061 ggaatataat tcaccgcttc ctgtgccgac aggaccggca ctcgtccatt aatacgaggt
     3121 ggttttacag gtttcattgt taattcctga ttcagatgtg attttcccta cgccagatgg
     3181 agaattagcg cacagagact cacgaaatat ttttattaaa tacggttaaa tatatttttt
     3241 aagttcggcg gggagggtgt tcccgccgaa atattattgc ttatgcctgg tgtaaaatag
     3301 ttaataccgt gcgtaaatca tttaccgaga tttgccctgg tgcagaggct ttttttaccg
     3361 caccaaaagt tgccgccgag ccaaatactt caccagccag acgagaaatt acgccagttt
     3421 ttgccatcga catcgtgatg attggacgat cggcatactg ctcctgcatc tccagggtcg
     3481 cggtaagcaa cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc ttaggaatat
     3541 cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat ttcttcggct tccggcgttt
     3601 tatggaagtc atggttggac atgacaactt tcacatcatg cgcgtgggcg taggcgacgg
     3661 tttctttaac ctgatcatcg ccggtaaata actccagatc gatcatatca accaggccgc
     3721 tgtcgatggc tgcacgattg agagcaatat aagcctcggt ggaaatcgcc tgctcgccgc
     3781 cttctttggc actgcggaag gtaaacagca gcggtttttc tggcatggtt tcacggagaa
     3841 tttttgccgc cgccatgaca gactccacat tggagaggtc ggcaaagtgg tccacacgcc
     3901 attccagaat atcaaagtcc gcttcacgat aggcgagagc ttcggatttc acgcgggcga
     3961 tatctttcgc catcagcgag acgatgattt taggtgcgcc cgtaccaatg acgagatctt
     4021 ttacagttac ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt caggcaccga
     4081 accccatgac ctgtttaaca tattccagag ggaaatcttt gccagtccat aacgtgaact
     4141 gttcagcccc ttgccacaac aacatgccgt atccatcaat cgttttgcaa ccagcttgtt
     4201 gcgcctgctg caataacttc gtcatatgcg ggttatacac gcattcagtg accagtagtc
     4261 ccggatgtaa cagactgata tcattaacta atgattcatt ctcaaggggt ttcataccca
     4321 cttttgtgcc attggttaaa atgtcggcgg aagccagggc ttcagcaaag gcttgctgat
     4381 cggcgagatc ggtaaccgtg acgacacaat cggtgttttc gttaacccgc tgcgcgaagg
     4441 cgagggcttt atcgaagaac tcatcccgac ggttaaagag tttaatttct tttaaacctt
     4501 caattgcccc ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac agcaccattg
     4561 ttttgccttt gatatcaaaa ccgctctctt taatggcgcg aatatggccc gtgccgtcgg
     4621 tgttatagcc gcgcaggtag ccatcatcat taacgatggt gttgatggca cccaccagtt
     4681 tggctgctgg cgttaattca tcaacatatt cacacgccag ttgtttgttc ggcattgata
     4741 taccagttcc gcgcattttg agggctttta atccttcaat tgctccggga aaactatcgt
     4801 tatccacttc aaaggccata taggtaaatg gcaatcccgc tttttctaag gctttattct
     4861 gcatttcagg cgataaactg tggcggatag gataggccat cagcccaatc aattcgtatt
     4921 ttgcggtaac atccataatt aactccttta acctctatgc ttaattgtgt ttaaccgggt
     4981 gctaaatttg cgctcgccaa accgcacatc attctcagga ataataaaaa cgcggtaata
     5041 gcggataaaa acaataattg cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac
     5101 aatgatatat tgcaggccga tgttcgacag ataaccggta atcagcggaa taacaaagtt
     5161 agccagtcca cccatcatca tataaatact ggtgactttg gctttgcttt ttgggaaaaa
     5221 ctctgacatg accgaaacac cgagctgtaa aatgccgcca gctgctgaga aaccgataac
     5281 aaaggcgccg gcattacaca ccagtggaga agggtacagg tagataatgg ctgctgttat
     5341 tgttgccagt gcagagttaa atacattagc ccagatgggc cggaccattt ttttcagtag
     5401 tgcggcaaaa ataaagacac agaccaacga gcccatactg taataagaga tggtttttaa
     5461 tgcctcagct tctgacatac cagcaaaagc catcgcatat ttgggcatcc acaccacaat
     5521 cacataaaag gtcgagaatg cggctacacc gaacagtacc gatgaaacac cttccagcca
     5581 gactaacggt ttgctgttca tttgcggtaa ttcattagct acgctggcgt ccaccaactg
     5641 gctggggaat ttgcttttca acagcatcag cgtgatcagt acaaacagaa tacccggaat
     5701 aatcagccca tagccgtacc agatattatt gagcaacata tagctcacca gcattgggta
     5761 gaacatttgc ccaaatgaca ccatcgcttt aaccagtatg accgccgaac cagaggcttt
     5821 cggaaagcat tccatgagcg cggggtagcc acccgtatcc agcgctgagt tagcgatacc
     5881 tacgcacact gccagaccgt aggcgagagt taaattcggg caagcgggaa taccaaagaa
     5941 gaatagcaga tacattatta ctgccattaa tatcaccgcc cgacgaccaa acttatcgga
     6001 gatcacaccg aagaataaaa tactgatcaa tcgccccaaa ccgataccgg aaattaagta
     6061 ggcaatgccc gcgttgtcag tggaaaactt ttctgccaga gatgacatat tttgggcaag
     6121 cgtaataaca ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa cagccaggat
     6181 aaatggcgtg ctgaaagcct tattttgaga cattttcaca gctccttgct gaaagataat
     6241 ttcgttaccg tacgtattgt tctccgacgg tttacataat tactatactg aagatgattt
     6301 tccctgggta catggcataa agtttatgtt tccaggaata ttgcatgtca tagtttgttt
     6361 ttgatcgtga ttttccttag ttagcgcaga aagagttatt tatccgtaag atcttgcatg
     6421 acctgaaaag aatattgcat tacccacccg gagcgacatt ttcctttagc gagtgatgcc
     6481 gcgttttttt gcggctgcgt aagccgataa acagtgccag taaaaagccg atggcagcga
     6541 tggcggtatc gaaccacata atatcggcaa tacttctttg ggacagatga gccgtaatca
     6601 acggaatagt aaaggtcgca atactgcccg cactgtaata gatccctgta gctttacctt
     6661 tagcgtaggg gaaacgttca gccattaacg tcaggccaat ttgcacaaca cctccagcag
     6721 aggtaaaacc aatgacaaaa gcaaatatta tcaccacata aaatgtggga tgcaggcaga
     6781 cggtaaacag agcaataaat gagataaagg tgtacagcat cagtaatgtt gttgggcgaa
     6841 cggtatttcg aatgagtgga gcggtaataa atacacaaag cagcgaaccc acggtgtaga
     6901 tactgagtag tttgatcgac atagtgtatg acatgcctgc aacaaattgt ccgtactgtg
     6961 acagccactg gctaaccaga taaaacgttg ccattgagat atagccatat aaggtataac
     7021 tggctaaatc gataattgaa cagcgatgtt ccgtagagcg ggtggttttc tttatgacag
     7081 gtaagcgacg acccggatgg ggtgggaacg tacaacggta taaaaacaga gcgttaataa
     7141 gcataatgcc tgcagcaatc ataaaggacc aaccgaacca cagttcagcc cacactaaca
     7201 ggctaatgat tagcggtaat aaaaattgtc cgctggaaac aaatgcttta attaaaatat
     7261 tggctgtccc aggtgagcgt ggaaaagctt ccatcaaact gggataagtg cctgcatcga
     7321 gaaaactgtt tgccattccc gccagaaagc caaaaacata agcgataatg atgttattgg
     7381 tctgcaggat gccaaaaaag aaggccatat agcaacacat cccgagcatg ataaaagggc
     7441 ggcgaccaaa gcgatcggat aataatcctg caaaaagcaa gacacttaat cgaccaatgc
     7501 ccagcgatga gataactatc gagacacccg cggcattagt ctgccaaagt gtctccagcg
     7561 aggccatatt caggctcatc aaaatgacgc ccataccatg caccaggtaa ttaaaataca
     7621 acccaagtgc ggtagggtaa tagggatttt tcataggttc tacttagcaa aggtgcttta
     7681 aaaagtgtag cgatgttgtg tcagtatcca ttcattattg ttgtaacaac gacaattcct
     7741 ttctgtggaa aaattagcaa agccgctcca gatcgtgtgc atataattca gcggctaccg
     7801 attgatagat cttccagtca ataaaattgc catcagggta gactcgttga aatgcggtta
     7861 agtcggggaa aaaacgcatg gtgttgttgc cgatgcggtt atttattttc tctatgatgg
     7921 catgaagatg tttttttctt tgctgacgca tagccaacag ttgctcgact acacgatcag
     7981 gaatggcaca cttgccattt tcccattgac gccagctttc actgtcaccg gtctgggcaa
     8041 tccaggtcgc gcattcatca atagtcatgg caaaaatatg gcggagtgcc tggagttcat
     8101 aagcgttcat cgtgtaatgg ctgcatgaaa taaacaataa aatataaata acattatcag
     8161 cctgctgacg gcttgaagag agtcacagta tcttgtgcaa cattattgga aagagtgagg
     8221 ggcgaggtag cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga tgggcaaaga
     8281 ctacacacag caattcgttg tttcactcag gggatttcca tggttataaa tcaatatgtt
     8341 gatttataac cgtgagctaa tagtaggcat acggactttt tgcgtcgatc agattcgtct
     8401 cattagttta aaaactgtaa agaatttgcg acaggtacta ccagctgaat ggcggtaagc
     8461 aggaaaatat ggggaagagc aagcaaatat caattagcgg tggttaacaa tcaggcggca
     8521 gagccgcctg atgagaaatt acttattcgg tttctcgatt tcgccaagtt cttcgagaat
     8581 ttcttccggt tggtccgtcg gcggcgggac ttcttccacc cacgcggcaa acagacgcca
     8641 ggaaacggct aacagaaccg gaccaataaa cagaccgatc atcccgaaag caatcaaacc
     8701 accaataacg ccagagagga tcaggatcag cggtaaatcg gcacccatgc gaattaacat
     8761 tgggcggatg acgttatcca gcgtgccaac cacaccgctc cacactaaca ataccgttcc
     8821 ccaggtggta tcgccggtcc agtagagcca gataatcgcc ggaatcagta ccggcaacgg
     8881 gccaagctgg acaaggcagg agaggatcat taacaccgtt agcaacgttg cataaggtac
     8941 gccggatacg gcgaggccga taccgccaag cactgcctgt actaacgccg tcaccaccac
     9001 acccagcgcc accgcgcgga tagcctgtgc cgccagcagc acggcggcat ctccgcgaac
     9061 gcctgccaga cgggttgcaa aatggcgaat gccttgtgcc acctgttcac cgcgccagta
     9121 cagcagggca ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc cgatatgcgc
     9181 cgcctgccca acgaaccagg tggtggtggt gccaatataa gggcggactt tcgccatgat
     9241 cgccgtcccc cccatatcca gcaagttgtg ccagcctgca tacagcttcg cgccaatcac
     9301 cggaatggta ttaagccacg ccagatcggg taacgtcatg tcgccgctgg aaatggcttt
     9361 aattagcggg ccgctgccgt cgacgatact gttaaccagc aaagcaatag ggatgataaa
     9421 caccatcact aataacagcg tcatcaccag aacggcgaga gagcggcggc caaacatgat
     9481 cttttgcaaa cgtaacaata ccggccaggt ggcgataacc accgtaccgg cccatgcaaa
     9541 gccgagaata aagggttgaa caatccacag acatgccaca atcatgatgg ctaaaaacag
     9601 caccgaaagc agaatttgtg cgacatccct gggctgacga acatttacca taactacttt
     9661 tcaccttttt tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact cacctgcata
     9721 atgatgagca atttcagcgg tttttaacag gccgattctg cccttaattc tgatgggcgt
     9781 gttagaaaaa aatgtggtac aacaactcag gcaacacgca aacgatttac tcgtcgtatt
     9841 tcaagttgca tgtgctgcgg ctacgttcgt tcaccccagt cacttactat tgtaagctcc
     9901 tggagttcac tcgcttgccg ccttcctgca actcgaaatc catagagtaa ataaacatca
     9961 cctttcagtg cgctattgca gccgcctgaa agaatagtgt atcgacctac atcacagacc
    10021 gcaggaaagg gtcaatataa tgattccaca gatttcccag gcacccggcg tcgttcaact
    10081 ggtgcttaat tttttgcaag agctggagca acaaggtttt accggcgata cggcgacaag
    10141 ttatgccgat cgtctgacaa tgtcgaccga caacagtatt taccaacttc tccccgatgc
    10201 ggtggtattt ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg ccgcgcagga
    10261 acgctattca tcgctgatct ttaccccccg cggcggcggc accggcacta acggtcaggc
    10321 gctcaaccag gggattattg ttgatatgtc ccgccatatg aaccgcatca tcgaaattaa
    10381 ccctgaagag ggctgggtgc gcgttgaggc cggggtgata aaagatcaac tcaatcagta
    10441 cctgaaaccg ttcggctact tttttgcgcc ggaactttcg accagcaacc gggcaacgct
    10501 cggtgggatg atcaataccg atgcatccgg tcagggatcg ctggtctatg gcaaaacgtc
    10561 ggatcacgta cttggcgtac gcgcggtgtt gttggggggc gatattctcg atacgcaacc
    10621 tttacccgtc gaactggcgg aaacgctggg taaatccaat accacaatcg ggcgaattta
    10681 taacacggtt tatcaacgtt gccgtcagca acgccagtta atcatcgaca acttccccaa
    10741 acttaaccgc tttcttaccg gttacgatct gcgtcatgtc tttaacgatg agatgaccga
    10801 gttcgacctg acgcgcattc tgacgggttc agaagggacg ctggccttta ttaccgaagc
    10861 gcggctggat attacgccct tgcctaaagt gcgccgtctg gtgaacgtca aatatgactc
    10921 ttttgactcc gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc tttcggtaga
    10981 gacggtggac tcaaaagtgc tgaatctggc gcgggaagat attgtctggc attccgtcag
    11041 cgagttgatt accgatgtgc ctgacaaaga gatgctcggg ctgaacattg tggagtttgc
    11101 tggtgatgat gaggcgctga ttgacgagcg agttaatgct ctctgtgtgc ggcttgatga
    11161 gctgattgcc agccaacagg caggtgtgat tggctggcag gtgtgccgcg agctggcggg
    11221 tgttgaacgt atctatgcga tgcgtaaaaa agccgttggt ctgcttggca atgccaaagg
    11281 tgccgctaag ccaattccgt ttgctgagga tacctgcgta ccgccggaac atctggccga
    11341 ttatattgct gaatttcgcg cgctgctcga cagccacggc ttaagctacg gtatgttcgg
    11401 tcacgtcgac gcaggtgtct tgcacgtccg tccagcgctg gatatgtgcg atcctcaaca
    11461 agagattttg atgaagcaaa tctctgatga cgtagtggcg ctgactgcga aatacggtgg
    11521 tttgttgtgg ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg cgtttttcgg
    11581 tgaggaactt tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc ataaccgact
    11641 caacccaggg aagatttgcc cgccagaagg tctcgatgcg ccgatgatga aagtggacgc
    11701 ggtgaagcgc ggtacattcg atcggcagat ccccattgcg gtacgccagc agtggcgcgg
    11761 tgcgatggag tgtaacggca acggtttatg cttcaacttt gatgcccgta gtccgatgtg
    11821 tccgtcgatg aagatcaccc agaaccggat tcattcaccg aaagggcgcg caacgctggt
    11881 gcgtgaatgg ctgcgtttgt tggcggatcg cggcgttgat ccactcaaac tggaacaaga
    11941 actgcctgaa tccggcgtca gtttgcggac gttaattgcc cgcacgcgca atagctggca
    12001 tgcgaataaa ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt cgggctgtct
    12061 ggcctgtaaa gcgtgttcga cccagtgccc catcaaaatt gatgtgccgg agtttcgctc
    12121 tcgttttctg cagctctatc acacccgtta tttacgcccg ctgcgcgacc acctcgtcgc
    12181 tacggtcgag agctacgcgc cgctgatggc acgcgcgccg aaaaccttta acttcttcat
    12241 taaccagccg ctggtgcgca aactctcgga aaaacatatc ggcatggttg atttgccgct
    12301 gctgtcggtc ccctcgctac aacaacaaat ggtggggcat cgctcggcaa acatgacgct
    12361 ggaacagctt gaatctctca atgcagagca gaaagcgcgc acagtgttgg tggtgcagga
    12421 cccctttacc agctattacg atgcgcaagt ggtggcggat tttgtccgtc tggtcgaaaa
    12481 attaggtttc cagcctgtgt tactgccatt ttcgccaaat ggcaaagccc agcatattaa
    12541 aggttttctt aatcgttttg cgaagacggc gaaaaagacg gcggatttcc tcaaccgtat
    12601 ggcgaagctg ggtatgccaa tggtgggcgt cgatccggcg ctggtacttt gttatcgcga
    12661 tgaatataaa ctggcgctgg gcgaggaacg tggcgagttt aacgtcttac tggcgaatga
    12721 atggctggca agcgcacttg agtcacagcc agtggctaca gtcagcggtg aatcatggta
    12781 tttctttggt cactgtactg aagttaccgc cttgccgggt gcgccagcac aatgggccgc
    12841 gatatttgcc cgttttggcg cgaaactgga aaatgtcagc gtgggttgct gcggcatggc
    12901 agggacttac ggacatgaag cgaaaaacca taaaaattcg ctcgggatct atgagttatc
    12961 ctggcatcag gctatgcagc gactgccgcg taaccgctgt ctggcgaccg gatattcctg
    13021 ccgtagtcag gtcaaacggg ttgaaggcac gggggtacgc catcctgtgc aggctttact
    13081 ggagattatt aaatgatatg gaaacgaaaa atcaccctgg aagcactgaa tgccatgggt
    13141 gaaggaaaca tggtggggtt actggatatt cgctttgaac atattggtga tgacaccctt
    13201 gaagcgacaa tgccagtaga ctcgcggaca aagcagcctt tcgggttgct gcatggaggc
    13261 gcatccgtgg tgctggccga aagtatcggt tccgttgccg gttatttatg taccgaaggt
    13321 gagcaaaaag tggttggtct ggaaatcaat gctaatcacg tccgctcggc acgagaaggg
    13381 cgggtgcgcg gcatatgtaa accgttgcat ctcggttcgc gtcaccaggt ctggcagatt
    13441 gaaatcttcg atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac cgccattttg
    13501 tgacgttgtc accagaaaag tgtgacgcat attgcttttg gtcattatct gcacagtaaa
    13561 cggtggtata ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac tcaacttgat
    13621 cccacccagc tggccattga atttttacgc cgtgaccaga gcaatctttc acctgcccaa
    13681 tatctcaaac gtctgaaaca actagaactt gaatttgctg acctgcttac tctctcttct
    13741 gctgaattaa aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg atcctgactt
    13801 aatgccgcaa gttcgccaaa tcgacctgga ttaacctccg tccaggttcg atgaccttct
    13861 taatactgct tatctaccgc ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt
    13921 aaaacggcgg gttagcttta tgagattatt cttatagccc cttcaagagc taagccactg
    13981 agagtgccgg agataagcgc cggatggggt agaaaccctt aagcctgtgt tgcacagact
    14041 taagggtttt cttatttctg aacgctgcac ttttttacct atctcttaaa tagtcatttt
    14101 caatacaact ttttcttttt catcacagcc ttaatgctcc ttagataata accattatct
    14161 aacaatgaga tacctaattc ttagcgtgcc tgttaaccca cacatcaggg tctatgctta
    14221 ttaaaagcag gcaaaaagca tgttttacgg taaagcccct gcgtttgctg ggttgtactg
    14281 ataatcatta tcactaacat gctgttatac gctgaaagcg atgaagtgag gtaaatcgat
    14341 ggacatgcat tcaggaacct ttaacccaca agatttcgcc tggcaaggct taacgctgac
    14401 acccgcagcg gcgatacaca tccgtgagct ggtggcaaag cagccgggta tggtcggcgt
    14461 gcgcttaggc gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg acagtgttag
    14521 cgagccggac aaagacgatc tgctgtttga acacgacggc gcgaagctgt ttgtcccgct
    14581 gcaagcgatg ccgtttattg atggcacgga agtcgatttc gttcgtgaag gacttaatca
    14641 gatattcaaa tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg
    14701 ggtataggcg gtactatgtc tcgtaatact gaagcaactg acgatgtcaa aacctggacc
    14761 ggcggcccgc tgaattataa agaaggattc ttcacccagt tagccaccga tgagctggca
    14821 aaggggataa acgaagaggt ggtgcgcgca atttcggcga agcgtaatga gccggagtgg
    14881 atgctgaagt ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga accgcactgg
    14941 ttgaaagcgc actacgacaa gctgaattat caggattaca gctactactc agcaccatcg
    15001 tgcggtaatt gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca aactggcgcg
    15061 aacgcctttt taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg
    15121 gaaggcaaag aggtggcggt ggatgccatt ttcgactcag tttcggttgc cactacttat
    15181 cgcgaaaaac tggcggagca gggaattatt ttctgttcct ttggtgaggc gatccacgat
    15241 cacccggaac tggtgcgtaa atatctcggc accgtggtgc cggggaatga caacttcttt
    15301 gccgcgctta atgcggcggt agcctctgac ggtacgttta tttatgtgcc taaaggcgtg
    15361 cgctgcccga tggaactttc cacctatttt cgcattaacg cggaaaaaac cgggcagttt
    15421 gagcgcacca ttctggtggc cgacgaagac agctacgtca gctacattga aggctgttcc
    15481 gctccggtgc gtgacagcta tcagttacac gcggcagtgg tggaagtcat catccataaa
    15541 aacgccgagg tgaaatattc cacggtacaa aactggttcc ctggcgataa caacaccggc
    15601 ggtattctca acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca
    15661 tggacgcaat cagaaaccgg gtccgcgatt acgtggaaat atcccagctg cattttgcgc
    15721 ggcgataact ccattggtga gttttactca gtggcgctga ccagcggtca tcagcaagcg
    15781 gataccggca ccaagatgat ccacatcggt aaaaacacca aatcgaccat tatctcgaaa
    15841 gggatctctg ccggacatag tcagaacagt tatcgcggct tagtgaaaat catgccgacg
    15901 gcaaccaatg cgcgcaattt cactcagtgc gactcaatgc tgattggcgc taattgtggg
    15961 gcgcatacct tcccgtatgt cgaatgtcgc aataacagcg cacaactgga gcacgaggca
    16021 acgacatcac gtattggtga agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa
    16081 gaagacgcca tctcgatgat tgttaacggt ttctgcaaag acgtgttctc agaactgccg
    16141 ctggaatttg ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga
    16201 taaggaataa acatgttaag tattaaagaa ttacacgtca gcgtggaaga taaagctatc
    16261 ctgcgtggat taagcctcgc cgttcgtccc ggcgaagttc acgccattat ggggccaaac
    16321 ggttcgggca aaagtacctt atcggcaacg cttgccgggc gcgaagatta tgaagtgacg
    16381 ggcggcacgg ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga agatcgtgca
    16441 ggcgaaggca tctttatggc cttccagtat ccggtggaga ttccaggcgt cagtaaccag
    16501 tttttcctgc aaacggcact taatgcagtg cgcagctatc gcggccagga aacgctcgac
    16561 cgctttgatt ttcaggattt gatggaagag aaaatcgctc tcctgaagat gccggaagat
    16621 ttattaaccc gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg caacgatatt
    16681 ttgcaaatgg cggtgctgga accggagtta tgcattcttg atgagtcgga ctccgggctg
    16741 gatattgacg cataaaaagt ggtcgccgat ggcgtgaact cgctgcgtga tggcaagcgc
    16801 tcattcatca ttgttacgca ctaccaacgc attctcgact acatcaagcc tgattacgtc
    16861 catgtgctat atcagggacg aattgtgaaa tccggcgatt tcacgttggt caaacaactg
    16921 gaggagcagg gttatggctg gcttaccgaa cagcagtaac gcgctgcaac agtggcatca
    16981 cttgtttgaa gctgaaggga caaaacgctc accgcaagca cagcagcatt tacaacaatt
    17041 gctgcgtacc ggactgccga cacgtaaaca tgaaaactgg aaatatacgc cgctggaagg
    17101 gctgaccaat agccagtttg tcagcattgc gggagagata tccccacagc agcgagatgc
    17161 cttagcgtta acgttagacg ctgtgcggct ggtatttgtc gatggacgtt acgtgtcagc
    17221 actgagcgat gcgactgaag gcagcgggta tgaagtgagc attaacgacg accgtcaggg
    17281 ggtacccgac gctattcagg cggaagtgtt tctgcatttg acggaaagcc tggcacaaag
    17341 cgtgacgcat atcgccgtga agcgcggtca acggccggca aagccattgc tgttaatgca
    17401 tattacccag ggcgtggcag gtgaagaggt caacactgcc cattaccgac atcatctgga
    17461 tctggcggaa ggtgccgaag caacggtgat cgaacatttt gtcagcctta atgatgctcg
    17521 tcacttcacc ggcgcacggt tcactatcaa tgtcgcagcg aacgcccact tgcagcatat
    17581 caagctggcg tttgaaaacc cggtcagtca ccactttgcc cataacgatt tgttgctggc
    17641 tgacgatgcc accgcattta gccatagttt cctgctgggt ggcgcagtgt tacgacacaa
    17701 caccagtacg caactcaatg gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc
    17761 ggtgaaaaac gaggtgtgtg atacccgtac ctggctggaa cacaataaag gtttttgtaa
    17821 cagccgacag ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt
    17881 gatcaacgtc gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca ataatctgct
    17941 gatgggcaaa ctggcagaag tggatacgaa accgcagctg gaaatctatg cagatgatgt
    18001 gaaatgcagc cacggcgcga cggtggggcg tattgatgat gaacagatgt tctatctgcg
    18061 ctcgcgcggg atcaatcagc aggatgccca gcagatgatc atttacgcct ttgctgccga
    18121 actgacggaa gcactgcgtg atgaggggct taaacagcag gtgctggccc gaatcggtca
    18181 acgactgcca ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc cgactttccg
    18241 gtgctttcgc gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc cgccagtgcg
    18301 cagaaaccaa gccaggtgat tgactccgaa gccgagtttt atcgtcacgg ctacgcggcg
    18361 gtgcatcgcg gtattcatac cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc
    18421 aagcgggcat cgctgtttat taatgcccgt tcggcggaag agctggtgtt cgtccgcggc
    18481 acgacggaag ggatcaatct ggtcgccaat agttggggca acagcaacgt gcgggcgggc
    18541 gataacatca tcatcagtca gatggagcac cacgctaaca ttgttccctg gcagatgctt
    18601 tgcgcacgcg ttggcgcaga gctgcgtgtg atcccgctca atcccgacgg tacgctgcaa
    18661 ctggagacgc tacctaatct gttcgatgag aaaactcgcc tgctggcaat tactcatgtc
    18721 tccaacgtgc ttggcacaga aaatcccctg gcggaaatga tcacgcttgc gcatcagcat
    18781 ggcgcaaaag tgctggtgga tggcgctcag gcggtgatgc atcatccggt ggacgttcag
    18841 gcgctggatt gcgattttta cgtattctcc gggcataaac tgtatggccc caccggaatt
    18901 ggcattcttt atgtgaaaga agccttgttg caggagatgc cgccgtggga agggggcggt
    18961 tctatgatcg ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc accatggcgg
    19021 tttgaagccg gtacacccaa taccgggggc atcattggtc ttggcgcggc gctggagtat
    19081 gtttcggcgc tggggcttaa taacatagcc gagtatgaac agaatctgat gcactacgcg
    19141 ctatcacagc tggaatctgt accggatctc actctctatg gcccgcggaa caggcttggc
    19201 gttattgctt ttaatcttgg taaacaccac gcctatgatg ttggcagttt tctcgataac
    19261 tacggcattg ctgtgcgtac cggacatcac tgcgctatgc cattaatggc ctattacaac
    19321 gtccctgcta tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga agtggatcgt
    19381 ctggtgaccg gactgcaacg tattcaccgt ctgctgggat aacagggagg cactatggct
    19441 ttattgccgg ataaagaaaa attgctgcgt aattttttac gctgcgccaa ctgggaagag
    19501 aaatatctct acattattga gctgggccag cgtctgccag aattacgcgc cgaagaccga
    19561 agcccacaaa atagcattca gggctgtcag agtcaggtgt ggattgtcat gcgccagaat
    19621 gcgcagggaa ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa agggcttatt
    19681 gcggtcgtct ttattctcta cgatcagatg acgccgcagg atattgtcaa tttcgatgtg
    19741 cgtccgtggt ttgaaaaaat ggcgctcacc caacatctca ccccatctcg ttcacaaggt
    19801 ctggaagcga tgattcgcgc aattcgcgcc aaagccgctg cacttagcta aactaaaggg
    19861 acagctttca tccggttggc tcacattgcc ggatggtcaa tgtttacact gacctgcaca
    19921 ggagtgtttt ggtttcaggt gaacataagc gccatccgac gtttcagcgt gagtctccgg
    19981 caaatacagg aggtttacaa tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc
    20041 ttttagcgcc atccaggctg cctgggcggt tgattatccg ctaccaccaa ccggaagccg
    20101 actggttggg caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc ttcaggctat
    20161 tgcccgacgt tttgatactg cggcaatgtt gatccttgaa gccaataaca ctatcgcccc
    20221 ggtgccaaaa cctggtacga cgataactat tccttcacaa ctgttattac ctgatgcacc
    20281 gcgtcagggg attatcgtta accttgcaga gctgcgcctt tattattatc cgccgggaga
    20341 aaatattgtg caggtttatc caataggtat tggattgcag gggctggaaa cgccggtgat
    20401 ggaaacgcgt gttgggcaga aaatccctaa cccaacctgg acgcctacgg caggcattcg
    20461 tcaacgttcg ctggagcgtg gcattaaatt accgccagtc gttcctgccg gaccaaataa
    20521 cccgctagga cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc tcattcatgg
    20581 taccagtgcg ccggacagcg tcggtttgcg cgtcagttca gggtgtattc gcatgaatgc
    20641 accggatatt aaagccttgt tctccagcgt gcggacggga acgccggtga aagtgatcaa
    20701 cgaaccggtg aaatattccg tggaacctaa cgggatgcgt tatgttgaag tacatcgacc
    20761 actatcagca gaagaacagc aaaacgttca gacaatgcca tacacactgc cagcaggctt
    20821 tacgcaattt aaagacaata aggctgtaga tcagaagtta gtcgataaag cgttgtatcg
    20881 tcgggcaggg tatccggttt cggtgagcag tggagcaact cccgcagcca gcaatgcgcc
    20941 ttcagtagag tcagcgcaga atggtgaacc agagcaaggg aatatgttac gcgcgacgca
    21001 gtagcggtaa acgggagaca aaaaaaatgg cgcacaatgt gcgccatttt tcactgcaca
    21061 ggtactatta cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg
    21121 cgtcatcttt agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt
    21181 caactttagc gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg
    21241 agcaacctgc cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt
    21301 tcattattaa taccctctag attgagttaa tctccatgta gcgttacaag tattacacaa
    21361 agttttttat gttgagaata tttttttgat gggaaggcac ttatttttga tcgttcgctc
    21421 aaagaagcat cgaacgctgg gttttagata aaaaaattga gagaaaacag gatgcttcca
    21481 tcggattcat cttagataaa gtgagattat atagtgaaat ccgatttgat tttttattgc
    21541 ttctggttat cgattaaata aaaaaagcgc ccatcagggc gcttcgatat acaaattaat
    21601 tcacaaaagc aatattacag gacgtgaaca gatgcggtgt tagtagtgcc gctcggtacc
    21661 agtgcaccag aaaccataac tacaacgtca cctttgtgtg ccagaccgct ctgcagagcc
    21721 agttctttac ccagacggta gaaatcatca gtagaagtga tctctttaac cagctgcggc
    21781 acaacgcctt tgctcagtac caactgatga gccgtttttt cgttggtggt cagtgccagg
    21841 atggtggcat ccgggaagta tttacgtact gcgcgagcag atttaccgcc ctgagtagca
    21901 accacgatca gcggagcatc cagtttttca gcagtttcaa cggcaccacg gcataccgct
    21961 tcggtaatgc gcagtttacg gttgtcattg ttgaactcga gacggctgtt catcacgcgg
    22021 tcggtacgtt cgcaaatggt cgccatgata gaaaccgctt ccagcgggta tttacctttt
    22081 gcggattcac cagacagcat cactgcatca gtaccgtcga ggatggcgtt tgcaacgtca
    22141 ccggcttctg cgcgagtcgg gcgtgggttt ttgatcatgg aatccagcat ctgggtcgca
    22201 gtgataacga ctttacgtgc acggatacat ttttcgatca tcatcttctg ggcgaagata
    22261 acttcttcta ccgggatttc tacacccagg tcgccacgcg caaccatgat gccgtcagag
    22321 gcttcgagga tttcgtcgaa gttgttgagg ccttcctggt tttcgatttt ggagatgatg
    22381 tggatgtttt cgccgccgtg cgctttcagg tgctcacgga tttcgataac gtcagaacgc
    22441 ttacgaataa aggaagcagc aacaaagtct acgccttgtt cgcaaccaaa gatcaggtcc
    22501 tgtttgtctt tttcagccag tgctggcaga gcaatggaaa cgccaggcag gttcacacct
    22561 ttgttttcgc ccaggtcacc gttgttcagc actttacaga taactttgtt accttcaatg
    22621 gcggtaactt ccataccgat cagaccatcg tcaaccagta cggtgttgcc aactgacagg
    22681 tcagtagtga aaccttcata cgttaccgca accatttcgc tgttgccgat aacagattta
    22741 tcagtggtga aagtaaaggt ctgaccagct ttcagagaaa cgtcgttacc gccttccagt
    22801 ttcatggtgc ggatttccgg acctttggta tcaagcagga tagcggcggt tttaccagtt
    22861 ttgctcatca cgttgcgcag attctgaatg cgctgaccgt gttctgcata gtcaccatga
    22921 gagaagttca gacgcataac gttcatgcca gcgtccagca ttttagctaa catctcttca
    22981 gattcggttt tcggtccgat ggtgcaaaca attttggtct ttttcatgac agtcttagtc
    23041 tttaagttga gaaggatggg agaaacttgc tttctgggcg cactgccgga aagcgaatcc
    23101 ggtattacga aagtggtggt gcctcgctct aaggataggt gacatcgaaa aagcgtgcag
    23161 aggaatgtgt gcttgtgtct cagcccaacg tgagtgcctg tttttgagtc gttgagttct
    23221 acagtccaaa gtgctgaaac cattcaagag tcaattggcg cgcattatac gctgaaatta
    23281 atccaaaagg aaaacaaaaa cagcgtttca aaaaatatac aacttgttag acaataaatt
    23341 gttatcaaag atttaacgtt ccagggaaaa ggttacgcaa aaacttcact gtttgcgcaa
    23401 cgtctggtgc cgaagttgtc agtgtgagga taagtgaaag ttcatccggt gtataaatac
    23461 atcatcgctc aattgctaca gttaaaataa tttatcgcgt tatttgtctg attttgtttg
    23521 atttttgttt aattggttag attgatatat gctgatagtt ggcatgataa ataggtataa
    23581 agaatgggaa atcggacaaa agaggatgaa ctgtaccgag aaatgtgcag agttgtcggt
    23641 aaagttgtgc tggaaatgcg cgatctgggg caggaaccaa agcatattgt tatcgcaggc
    23701 gtattacgta cagcattagc caataaacgc attcagcgca gtgagctgga aaagcaggcg
    23761 atggaaaccg tcattaacgc actggtaaaa tgaccattca ggatgaggcg gtaagcttca
    23821 tcctgatatt tttgcttcag ttgcggtcca gttaatcatt ctaaataaca actgctgaaa
    23881 tt
//
LOCUS       DAZFVO020000072        23690 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFVO020000072 DAZFVO020000000
VERSION     DAZFVO020000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.072"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..82)
                     /locus_tag="ACHKNB_RS13085"
                     /old_locus_tag="pgaptmp_002617"
     CDS             complement(<1..82)
                     /locus_tag="ACHKNB_RS13085"
                     /old_locus_tag="pgaptmp_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196105.1"
                     /translation="MRYNGLNNMFFSLCQINDNHSFTSSSH"
     gene            complement(260..1909)
                     /gene="actP"
                     /locus_tag="ACHKNB_RS13090"
                     /old_locus_tag="pgaptmp_002618"
     CDS             complement(260..1909)
                     /gene="actP"
                     /locus_tag="ACHKNB_RS13090"
                     /old_locus_tag="pgaptmp_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HGU4196106.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1906..2220)
                     /gene="yjcH"
                     /locus_tag="ACHKNB_RS13095"
                     /old_locus_tag="pgaptmp_002619"
     CDS             complement(1906..2220)
                     /gene="yjcH"
                     /locus_tag="ACHKNB_RS13095"
                     /old_locus_tag="pgaptmp_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HGU4196107.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2420..4378)
                     /gene="acs"
                     /locus_tag="ACHKNB_RS13100"
                     /old_locus_tag="pgaptmp_002620"
     CDS             complement(2420..4378)
                     /gene="acs"
                     /locus_tag="ACHKNB_RS13100"
                     /old_locus_tag="pgaptmp_002620"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HGU4196108.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4771..6207
                     /gene="nrfA"
                     /locus_tag="ACHKNB_RS13105"
                     /old_locus_tag="pgaptmp_002621"
     CDS             4771..6207
                     /gene="nrfA"
                     /locus_tag="ACHKNB_RS13105"
                     /old_locus_tag="pgaptmp_002621"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HGU4196109.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6252..6818
                     /gene="nrfB"
                     /locus_tag="ACHKNB_RS13110"
                     /old_locus_tag="pgaptmp_002622"
     CDS             6252..6818
                     /gene="nrfB"
                     /locus_tag="ACHKNB_RS13110"
                     /old_locus_tag="pgaptmp_002622"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HGU4196110.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6815..7486
                     /gene="nrfC"
                     /locus_tag="ACHKNB_RS13115"
                     /old_locus_tag="pgaptmp_002623"
     CDS             6815..7486
                     /gene="nrfC"
                     /locus_tag="ACHKNB_RS13115"
                     /old_locus_tag="pgaptmp_002623"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HGU4196111.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7483..8439
                     /gene="nrfD"
                     /locus_tag="ACHKNB_RS13120"
                     /old_locus_tag="pgaptmp_002624"
     CDS             7483..8439
                     /gene="nrfD"
                     /locus_tag="ACHKNB_RS13120"
                     /old_locus_tag="pgaptmp_002624"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HGU4196112.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8459..10177
                     /gene="nrfE"
                     /locus_tag="ACHKNB_RS13125"
                     /old_locus_tag="pgaptmp_002625"
     CDS             8459..10177
                     /gene="nrfE"
                     /locus_tag="ACHKNB_RS13125"
                     /old_locus_tag="pgaptmp_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HGU4196113.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFHFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10170..10553
                     /gene="nrfF"
                     /locus_tag="ACHKNB_RS13130"
                     /old_locus_tag="pgaptmp_002626"
     CDS             10170..10553
                     /gene="nrfF"
                     /locus_tag="ACHKNB_RS13130"
                     /old_locus_tag="pgaptmp_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HGU4196114.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10550..11146
                     /gene="nrfG"
                     /locus_tag="ACHKNB_RS13135"
                     /old_locus_tag="pgaptmp_002627"
     CDS             10550..11146
                     /gene="nrfG"
                     /locus_tag="ACHKNB_RS13135"
                     /old_locus_tag="pgaptmp_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HGU4196115.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11488..12801
                     /gene="gltP"
                     /locus_tag="ACHKNB_RS13140"
                     /old_locus_tag="pgaptmp_002628"
     CDS             11488..12801
                     /gene="gltP"
                     /locus_tag="ACHKNB_RS13140"
                     /old_locus_tag="pgaptmp_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HGU4196116.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12879..13568)
                     /gene="yjcO"
                     /locus_tag="ACHKNB_RS13145"
                     /old_locus_tag="pgaptmp_002629"
     CDS             complement(12879..13568)
                     /gene="yjcO"
                     /locus_tag="ACHKNB_RS13145"
                     /old_locus_tag="pgaptmp_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HGU4196117.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13662..15809)
                     /gene="fdhF"
                     /locus_tag="ACHKNB_RS13150"
                     /old_locus_tag="pgaptmp_002630"
     CDS             complement(13662..15809)
                     /gene="fdhF"
                     /locus_tag="ACHKNB_RS13150"
                     /old_locus_tag="pgaptmp_002630"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15390..15392),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HGU4196118.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(16007..17473)
                     /gene="mdtP"
                     /locus_tag="ACHKNB_RS13155"
                     /old_locus_tag="pgaptmp_002631"
     CDS             complement(16007..17473)
                     /gene="mdtP"
                     /locus_tag="ACHKNB_RS13155"
                     /old_locus_tag="pgaptmp_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HGU4196119.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLALDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17470..19521)
                     /gene="mdtO"
                     /locus_tag="ACHKNB_RS13160"
                     /old_locus_tag="pgaptmp_002632"
     CDS             complement(17470..19521)
                     /gene="mdtO"
                     /locus_tag="ACHKNB_RS13160"
                     /old_locus_tag="pgaptmp_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HGU4196120.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAKGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19521..20552)
                     /gene="mdtN"
                     /locus_tag="ACHKNB_RS13165"
                     /old_locus_tag="pgaptmp_002633"
     CDS             complement(19521..20552)
                     /gene="mdtN"
                     /locus_tag="ACHKNB_RS13165"
                     /old_locus_tag="pgaptmp_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HGU4196121.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20571..20846)
                     /gene="ytcA"
                     /locus_tag="ACHKNB_RS13170"
                     /old_locus_tag="pgaptmp_002634"
     CDS             complement(20571..20846)
                     /gene="ytcA"
                     /locus_tag="ACHKNB_RS13170"
                     /old_locus_tag="pgaptmp_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HGU4196122.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(21055..23040)
                     /gene="yjcS"
                     /locus_tag="ACHKNB_RS13175"
                     /old_locus_tag="pgaptmp_002635"
     CDS             complement(21055..23040)
                     /gene="yjcS"
                     /locus_tag="ACHKNB_RS13175"
                     /old_locus_tag="pgaptmp_002635"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HGU4196123.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23293..23610
                     /locus_tag="ACHKNB_RS13180"
                     /old_locus_tag="pgaptmp_002636"
     CDS             23293..23610
                     /locus_tag="ACHKNB_RS13180"
                     /old_locus_tag="pgaptmp_002636"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGU4196124.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         5398 a         6157 c         6435 g         5700 t
ORIGIN      
        1 tatgtgatga acttgtgaaa gagtggttat cgttaatctg gcaaagagag aaaaacatat
       61 tatttaaacc attatagcgc ataaaatatc cttataatta acaagaaaga aaaggcatat
      121 tctctgcatt atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt
      181 gtttaatatt atgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc
      241 gaccggggaa ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg
      301 agcggataaa ctgtgcacgg aacagttcac gctcgcgcgc accttccgct gagttatcgg
      361 ttgccgagaa gaaccagatg ccgaggaatg ccacgctgat agagaacagc gccgggtatt
      421 catacgggaa gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga
      481 tcatcagtac cactgcggta atcagcccca gccagccacc catcatcgcg ccacgagtgg
      541 tcagtttcga ccagtacata gaaagcagaa tgatcgggaa gttacagctc gctgcgatgg
      601 caaacgccag gcctaccatg aaggcgatgt tctgattctc aaacagcacg ccgaggataa
      661 tggcaatcac acctaaaatc agcacggtga ttttcgatac ccgcagttct tcacgttcgg
      721 tcgcgccttt tttgaagacg ttagcgtaca ggtcatgcga aaccgctgat gcgcccgcca
      781 gcgtcagacc agcaaccacc gcaagaatgg tggcaaaagc aaccgctgaa ataaaaccga
      841 ggaacaggtt gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa
      901 tcagatggcc cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga
      961 agccgataat aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc
     1021 tcttacgtgc ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc
     1081 ccgccgtacc aaacatcagt cccagaccca gagagagcgc ggagatcgga tctttcacca
     1141 gcccgcccgg cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca
     1201 gattgttgaa gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga
     1261 acagcaacag cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca
     1321 ggacgtacat catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa
     1381 acagcagctc gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca
     1441 ccaccagaga accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg
     1501 ccacatcggc aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa
     1561 tgatcggcca gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa
     1621 acaccagcgc ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca
     1681 gcccgttctg gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta
     1741 cgcgttttga tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa
     1801 taatcgcctg ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt
     1861 tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac
     1921 ctcatgcagg acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat
     1981 accggtgaga acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac
     2041 gctggtgttc ggattcagcg gcgtgcccag ccagccgggc gcgaaggcga tcagtaaaat
     2101 aaagccgata taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt
     2161 ttcgactaac tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat
     2221 cacagattct ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag
     2281 gttaacgctc gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg
     2341 gcaatcaatg cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact
     2401 cgcatccggc aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct
     2461 tctcgactac gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg
     2521 ccgcaatttt gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt
     2581 cggtccagtg cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc
     2641 ggacttctgc gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt
     2701 agatcgcctg acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg
     2761 gatgcgccac cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca
     2821 gcacgtcgtc cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt
     2881 cgccgctgaa atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat
     2941 cgccaaacag cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg
     3001 tagcgccttc cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg
     3061 gacgtgttgc cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac
     3121 cgccagtttc ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt
     3181 tccagtacca ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca
     3241 gcgacgaacg gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg
     3301 ccgtgggtgc ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg
     3361 caggcgtcgg ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggccagcg
     3421 ggccgtacag caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga
     3481 tatcacccgg atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac
     3541 cgccggtagt atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa
     3601 acagcggatc ttcggcgttc atctcttccg cctggtgctg atcgctcgct tgctcaacca
     3661 ggtcgtgcca ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca
     3721 gtaccaccac atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt
     3781 tcttcagcgg aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg
     3841 agttggaatc aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg
     3901 aatgcaccgc gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg
     3961 gcatataaat cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga
     4021 agcggcagac gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt
     4081 cgccttccca gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt
     4141 ttgccgccag attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa
     4201 aggaggtgtt tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc
     4261 cccagaaggt atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt
     4321 ttatcaggca acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc
     4381 ttttgttctc cttgtaggat gttaataata tgtggcataa gcgttaaatg taggggtatt
     4441 ggcagttttg tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac
     4501 ggcagggtaa tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag
     4561 ttatgcatgc tgttgaaaaa gaggaagata ctgactaact ctaaagtggt attttacatg
     4621 cacttacaat tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga
     4681 gcaatgtcat gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg
     4741 caacaatggc gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt
     4801 atcttcagct tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggct
     4861 gctcccgcaa aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc
     4921 gatcaatatc tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg
     4981 gaagatccac ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag
     5041 ccgcgtggac atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg
     5101 aaaaacgctg aagatggtcc gctaccgatg gcatgctgga gttgtaaaag cccggatgtg
     5161 gcgcgtctga tccagaaaga cggcgaagat ggctacttcc acggtaaatg ggcgcgcggc
     5221 ggcccggaaa tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag
     5281 ttcgccaaag gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa
     5341 gccattggta aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt
     5401 cagtgccatg tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat
     5461 gacggcatga aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg
     5521 actaactccc tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg
     5581 acagcgggca ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg
     5641 cagaacgccg aaggcaaact ctacaccgac cataaaattg gtaatccgtt tgataacttc
     5701 gcccagactt gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg
     5761 gaacgtaagc agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct
     5821 cacttcgaag cgaaagcagc gctggatgca ggcgcgacgg aagccgaaat gaagccaatt
     5881 caggacgata tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt
     5941 catatgcacg caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg
     6001 gatgcacgca ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag
     6061 atcccggata tctcaaccaa agagaaagcc cagcaggcta ttggcctgaa catggaacaa
     6121 atcaaggccg agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca
     6181 cgtaaaaacg gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca
     6241 atggagtgaa tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc
     6301 tgttgtggag cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct
     6361 acgaagttac ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aagccagata
     6421 ccgaaggtat gcatggaaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca
     6481 cctgcaccaa ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga
     6541 tgcgctttaa cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc
     6601 atctgccgga acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg
     6661 cgtgcgccag ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca
     6721 aaggacggat taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta
     6781 acccggcgtc tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct
     6841 caccggcgtc ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac
     6901 gttgaatatc aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg
     6961 taccgcctgt atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct
     7021 gacgattatt cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg
     7081 taagtcttgc cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc
     7141 ttttcgtgat gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca
     7201 gtactgcatc gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc
     7261 ggacaaatgc gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgcgt
     7321 tgaggcttgc ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat
     7381 ttcgcaactg ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc
     7441 gaagctgtac cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca
     7501 tttcattttg aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt
     7561 atttctgccg gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc
     7621 ggtgcagaca gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc
     7681 ggtctgttga tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc
     7741 cactacagtt ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg
     7801 gtgctggtgc tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc
     7861 tggttgccga agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc
     7921 ggactggaaa cattgatgct ggtgttggcg gtgttgttgg gggcttatac cggctttctg
     7981 ctgtcggcgc tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc
     8041 ttctccggca tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc
     8101 agtaacccgc attccacgga agcgcagttt gtacaccgta tggaaatccc cgtggtatgg
     8161 ggtgaaatct tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa
     8221 gtgcgtgcgc tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt
     8281 gtcgccgggc tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc
     8341 ggcattcctg ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc
     8401 tttttcattc tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct
     8461 ggacgtattc cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt
     8521 gttgaccccg ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact
     8581 cacttgcatc ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac
     8641 gtattgtttt ctcatcagcg atttctcggt catttatgtc gcgcaacata gctatagcct
     8701 gctgtcgtgg gaactcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct
     8761 ttgggtgctg ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac
     8821 cgatccgcta tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact
     8881 gtttgtggtg ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg
     8941 cgatctcaat ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct
     9001 tggctatggc ggtttgatgg tagcggcgag cgtggcgctg gcgagtttac tgcgcggcga
     9061 gtttgatggt gcctgcgcca gaatttgttg gcgctgggca ctacctggct ggagcgcatt
     9121 aacggcgggg atcatcctcg gttcctggtg ggcctattgc gaactgggct ggggcggctg
     9181 gtggttctgg gatccggtgg aaaacgcctc tttattaccc tggctttctg ccactgcgct
     9241 gctgcacagt ttgtccctga cacgccagcg ggagattttc cgccactggt cgctgttgct
     9301 ggcgatagta actctgatgc tgtcgctact gggtacctta attgtccgtt ctggcattct
     9361 ggtttcggtt catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt
     9421 tgcactgatt agccttgcgt ctctggctct gtatggctgg cgagcgcggg acggtggccc
     9481 ggtggtgcgt ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt
     9541 ttgcgcagtg ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct
     9601 gggctgggga cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg
     9661 cctgttgatg ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgtgcagct
     9721 tccggcgctg gtagctcatg caggcgtgct gttatttgcc gcagggatcg tggtttccag
     9781 cgtcagccgt caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg
     9841 atacaccttc cattttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa
     9901 agcgatagtg gcactgtttg accatcagca acgcattggt gaactgatgc cggagcggcg
     9961 tttttacgaa gcacgtcgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca
    10021 tgactggtat gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta
    10081 tgtacaaagc ggtgtgcgct ggatctgggg gggaggattg ttgatgattg cgggcgcatt
    10141 gttaagcgga tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt
    10201 tatttacctg ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac
    10261 aacagcaaca ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa
    10321 acttactgga atccaacgcg ccggtggcag tcagtatgcg ccatcaggtt tacagcatgg
    10381 tggcggaggg gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt
    10441 ttgttcgcta taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg
    10501 tgttgttact gctgatggca ctgatcctct ggcgagtgag ggcgaagcga tgaaacagcc
    10561 caaaataccg gtgaaaatgc tgacaacact cacgatattg atggtatttc tctgtgtcgg
    10621 cagttatctg ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga
    10681 tccgctacat cagtttgcca gcaagcaaac cccggaagcg cagcttcagg cattacagga
    10741 taaaatccgc gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct
    10801 gtggcaaaac gattacagca attcgctgct ggcgtaccgt caggcgttgc aactacgtgg
    10861 tgagaacgct gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca
    10921 tatgaccgcc cagactcgcg caatgatcga caaagccctc gcgctggact ctaacgaaat
    10981 caccgctctg atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat
    11041 cgaactatgg caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt
    11101 cgagtcgatt aatatggcga aattgttgca gcggagatca gattaatcat ctggtgatga
    11161 agattatgtt attgagttgt ataaaaaata atcgaatgaa catatgccaa aaataatcac
    11221 caatcagtat tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat
    11281 taatcctgcc ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa
    11341 gggttgcgca acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac
    11401 caacgcaaca caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa
    11461 cggcgctcca ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag
    11521 attctgtttg ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac
    11581 agccgcgact ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg
    11641 attaaaatga ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtg
    11701 ggtgatgcca aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc
    11761 accaccgtcg ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg
    11821 gtcgatatgt cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg
    11881 gtacaaagca gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt
    11941 gtggcgtcga tggcgaaagg cgaaatgctg ccgatcatct tcttctcggt gctgtttggt
    12001 ctggggcttt cctccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc
    12061 atctctgaaa ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg
    12121 tttgcgctga ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg
    12181 aaactggtgc tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt
    12241 gtggcgcgcc tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg
    12301 attctggcgt actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg
    12361 gaagcctacg gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt
    12421 aaccttgatg gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat
    12481 ggcattgacc tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg
    12541 aaagggattg ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc
    12601 gtaggtattc cgctggaagg cctggcgttt attgctggtg ttgaccgtat cctcgacatg
    12661 gcgcgtactg cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg
    12721 gaacacaaat ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt
    12781 gataaaactg cggatcaata attgaagatt gccggggtta tccacccggc aatgtgtgga
    12841 tgcctgatgc gacgctggtg cgtcttatca ggcctacctt aaccgttggt taatttttca
    12901 aactcttcac acccggtatc aaacccttcc atacagctca ggttcaacca gtgcagcgcc
    12961 ttttgtttgt tcttctcgat aaaaccttcc tcaccgttta agaacatcat tcccgcccag
    13021 tactcggaat aaccggttcg ggaaattgca gagctgcgtt tgaaatacca ggttgccttg
    13081 tcatcgtcgg ccttaatgcc cacgccgttg gcgtaaatca aaccaagcag catttgggca
    13141 tcgacggcgg agtcattctc cagatcttcc gaggcgtttt ctaacagcga aatcgccttt
    13201 ggataatccg gtttacccgc ttgagtattt accagaatat gcgccagcgt tacttcacct
    13261 tctttactgc ccgcttgcgc cgctttctct gcaagaactt ttgcttgtgg atagtccaga
    13321 ctgaccggat tggtgatttt gatctgcgcc agcagcgcgc aggcatcagc atcaccgctg
    13381 tcggcggctt tctgtgccca atattcggct ttgctcaaat cgccggagct aaaccagctg
    13441 tcggcaagaa aatattgtgc gcgtcggtcc ccggcctctg ctgcctttaa atactggctg
    13501 ccaggctcgg agtcgttcgc gtgggcaaag aatgtcaaaa acaacatcaa tgcgataatt
    13561 tttttcattt tgataatacg attgggtagg gagtaaccag tataaagagt tcgaatggat
    13621 aaaaaaacag cctccgaaag gaggctgtag aaaggactat attacgccag tgccgcttcg
    13681 cgcaggcgag ttttcaactt gttgtactcg tcaatcacgt actgctcggc ggcgcgctga
    13741 tcggcgatcg gctcgacgcg cacggcgcag tatttgtatt ccggcgtttt cgtaatcggg
    13801 cttaagtttt cggtaaccag ctcgttacag gcaccaatcc accactggta ggtcatgtaa
    13861 atcgcccctt tgttcggacg atcgctgacc tgcgcacggg tgataatttt gcctttacgc
    13921 gagtgcaccc aaaccaatgc ctcatcttca atacccagac gtttggcgtc ttcggtattg
    13981 atttgtgcgt agccaggttc atcagccagc gccgccagtg ccgcacagtt accggtcatc
    14041 gaacggcaag agtagtgacc aacttcacgc accgttgaca gtaccatcgg gtactcgtcg
    14101 gtgagtttgt cgattggcgc tacccagtcg caggtgaaga actgtgccag accgttcggg
    14161 gtatcaaact tctctttaaa cagataagaa gtcccctgat cggcatctga agtatcgcgg
    14221 caaggccact gaatgaagcc cagttcgccc attttctcgt aagtcgcacc gtagaaatcc
    14281 gggcacagat gacgcaactc atcccagatc tcctgggtgt tgttgtagtg catcggataa
    14341 cccatacggg tggcgatttc actgatgatt tgccagtccg ttttcagatc ccatttcggt
    14401 tcaactgctt tgaagaaacg ctggaagcca cggtcagccg cagtaaacac gccttcatgc
    14461 tcgccccacg acgttgacgg caaaatgaca tccgccgccg acgcggtttt ggtcataaag
    14521 atgtcctgaa cgataaccag ttccagatct tcaaaggctt tacgtactgc cgacagctcc
    14581 gcgtcagttt gcagcggatc ttcgcccata atgtacgcgg cacgcacttc gccatgcgct
    14641 gcgcggtgcg gcagctcgct gatgcgataa ccggtatgag caggcaggct ttccacgccc
    14701 caggctttgg cgaatttctc gcggttagcc ggatctttca cgtactggta tcccggatac
    14761 gtatccggca gcgcgcccat atcgcaggca ccctgcacgt tgttctgacc acgaaccggg
    14821 tttacacccg catgcggctt accgaggtta ccggtcagca tcgcgaggct ggtcagagaa
    14881 cgcacggttt ccacgccctg gtagaactgg gttacaccca tgccccacag gatggcggcg
    14941 cttttcgcct gggcatacat ccgtgccgcc tgacgaatct cactggcgct gacgccggtg
    15001 atatcttcaa ccgactccgg cgtgtagcct tcaacgattt tacgatactc ttcaaagcct
    15061 tctgtacggg aagcgacgaa cgctttgtcg tacagatttt cttcaataat gacatggccc
    15121 atcgcattca acagcgcgat gttcgagccg tttttcagtg caatgtgcat gtcagcaatg
    15181 cgcgcggttt caattttgcg cggatcgcag acgataattt tcgccccgtt acgtttagcg
    15241 ttaattacgt gattcgccac gattgggtgg gaatccgccg ggttgtaccc gaaaacgaac
    15301 actaaatcgg tattatcaat ttcgttaata gcattgctca ttgcgccatt accgaccgat
    15361 tggtgcagac ctgcaaccga tgggccgtgt cagacacgag cgcagcagtc aacgttattg
    15421 gtaccaataa cggcgcgcgc aaatttttgc attacatagt tggtttcgtt acccgtacca
    15481 cgcgaggagc cggtcgtctg gatggcatcc ggaccgtact tctctttgat ggcgctcagg
    15541 cgctcggcaa cgtaattcag tgcctcatcc caggaaacag gttcgagttt gccgccacgc
    15601 tggcgacgga tcatgggggt tttcaggcgc ggggtcagga tctgggtatc gttaatgaaa
    15661 tcccagccat aataaccctt cagacacagg gtaccctggt tggttttccc ctgcgctgcc
    15721 tccgcccgga cgattttgcc gttatcgacg accaggttga ttttgcaacc tgatgcgcaa
    15781 taggggcaaa ccgtgacgac ttttttcatc ggtctcgctc cagttaatca aatcacgcat
    15841 acgcgctctc gactacagta tgcatctttt atgccacatt ttatttgggg tcattccctg
    15901 atattacggg cactatttat tcaaaactct gacgaaaaac aggctgtcgt cagttttgac
    15961 gtgacgaaac gaaataccgc gtgacagcca tcacgcggca gacattttat tttttctcga
    16021 cgacgggagc cgcctgatac ccgccgccca gcgatttcat caactgaatg ctttggatca
    16081 cccggcggct gtccagcatc agtaatgaca tctcttcggc aagtactggc aaccgggctt
    16141 cagtggcctg taagcggctg gttaagccgc gctgataggc ggcctcggca gcgcgctggg
    16201 tgaagcgcgt tgcttccacg cgttcagcct gcatttctcg ctcgtcgttg agcgtttgca
    16261 gacgcgtgcc gttgacggca acgtcacgca ccgcgttcag tactgactgg ttgtaacgtt
    16321 caatcatcat gttgctggcg gcgcgcgtgc cttcgagatt ggcattcaac cgtccaccgt
    16381 caaacagcgg caatttcaga cccgggatga agttgaactg gcgactggtt tttttgaata
    16441 aggtatccag gtggatggca tcaaggccga aaaacgcttt gatatcaaag ctcgggtaga
    16501 acagcgcccg cgcggaatcc acctgatcta atgacgcctg aacataccag cgcatggctt
    16561 gcagatccgg gcgtctggcg agcaactcat aagagagtgt cgccggaatg ccggtctgga
    16621 ctcgtggtaa tgccaccggt ttgatctccg gcatatcgct ggcccccgcg ccaatcaatg
    16681 cacgcagaga ttctcgcgtt tcggtgattt gccctttgac ggcagcaatt tgtttatcga
    16741 ccgccagaat ctgtgcccgc gcgccgtgga aaggcacttg tgcttccaga ccgtgcgcca
    16801 ctttactctg gtgcgctttc accgcgtaat caatcacatc gcgagtttgt tctaacagat
    16861 cgagcatctg atagctggcc tgcatactgt aataaagctg cgctacgccc gtggtcagcg
    16921 atagctctac tgctgcggtt tctgccagcg cggcattatg cgcgccaatg gcggcggcaa
    16981 ccgctgagcg atgcacaccc cacaaatcaa gagccagtcc ggcaaacaaa cctactgtgg
    17041 cttccgtata gtacggcccg tccataccca gtgcgggcgc atccatcgca taagggctta
    17101 aaaagccgtt cgccgagacg cgttggcggt tgagcatccc taacgctgcg acctgtaatt
    17161 gtgaaccggc atctaacaaa tcggcctggg actgcgcttt ttcttcccgc agtttcgctt
    17221 cggcgagggt gtgtgaacca cttagcgtcc gttggatcag cgcatccagc tgcgggtcat
    17281 tgagttgttt ccaccactgc gcctgcggcc agccggagct ggcaagatga atatcgtcgg
    17341 ccagtttgat ttgttccggt ttgagctgtt gatgaggtgc agaatcctta cgtaccaggg
    17401 cacagccgga aatcagcgtc gtgctgccga gaatgctgca caacagcaga cgtgaaagtt
    17461 gacgattgat cattgcgtgg ctccttgtgc ctgttccgtg gcgggcgtta atgccggggc
    17521 tgtccagtct ggcaagcggg cgaaaagctg gcagacgtgt tgctcctgtt ttaataaggt
    17581 gggcaggatg gcctgcgagg cgggtgtctc ttcaagcgtt atttgaggag aacggctgag
    17641 tgcggttgcc agaccggcag cgtatttctc cagggcgtcg gcaaactgag ctgctctgtc
    17701 cggctgtagt gcgttatcca gcgcctgcgc cgagttccag gtggcatccc aggcgtgaag
    17761 aatatcgcgg ccctgacgaa taaccgtttg cgaacgttca atcagtaagg cgcgttcttc
    17821 gctgtccagt tgacgctcca gcgccacgcg ttggcacatc tcttcacagg cattaaacgc
    17881 cgcatgcaga ccgatacgaa tttgcagata agtgcgcaga gccgtgactt cctgctggcg
    17941 tgggatccgc attactttac ttaacatacc cagcgtgcca gccagttttt gcggcagtgt
    18001 gcgcgcttca ctttcaggcc agacaaaggt gtaaatcacc gcggacacca cggtaccaat
    18061 gatgataccc atggcgcgat cgcgaatttc caccaggtcg tacaccgggc caaaaacgtt
    18121 ttcgagcgtg gcgagcgcga aggtgaccac catctgtgtg ccgatataag aagagcgttc
    18181 agagctggtg gcaatccatg cgcccaacag gaaaatcggt gccagcacaa acagcaattc
    18241 gacaatattg tccagccagg gcatgaccag tagcgtgaat aacagcgcca gaatcgcgcc
    18301 gcaaaaggcc ccgccaaaac gcagcaccat cttctggtac gacgaaccga catttgggtt
    18361 agcgacgatc acgcatgtca gcatacaggt gtgaatgcct tcccagtcca cgccgctgta
    18421 gaaggtgtaa cagatcaaac aggcgagcag tgtttttacc gcgtagcgca tatagtctgg
    18481 attggtaaaa gcatcggcga ccattgatgg cggtttggcg gcgggcgttg gcggcgtatt
    18541 cgggtccatc tggcccagtt gtaacaacgt ctggcagata ttctccaggt tacattcccg
    18601 tgccgccatc gcttcacttt cagtgatccg ccagtcgctt tgccagcact gacctttagc
    18661 aacggcatgc tgcagcttat tgatttctga ggctaatttt tgccgaaatt caataattgc
    18721 ctgagaatca gcaaaagagg tgggatcgta gcgattcagc gtcgagtaaa tgtaggttac
    18781 cgttgccacg cagctttgcc accatgcgct ttgagttcgc cagttggcat cgtccgcgag
    18841 acaaaagaca ttcagttttt gcagcgccag cgcctctctt tcaatccgcg tttcgggtag
    18901 cggtgcgagg ctgtccgtca ggtgactaat ggcatcatca agccgatcat taagcgcttg
    18961 atgcatttgc gtaatggcac gactgggaaa ccacagcacg ccgattaacg tcatcagcaa
    19021 ggttggatag aggccaacaa cgatacacca cagcgttaag cgcacgacca cttccggata
    19081 gtcgagcatg gcggggaagg tttgcccgta aatggcgaca atggcgacag cgaaaaagac
    19141 cagtcccagc cgatgggtgc gcatcaaaaa catacagccc atcaggatcg gcccggcgat
    19201 gatcaatcgg atcaacggtt cgccgtatga ccatttgtag atcaaaaaca ggctgccgat
    19261 ctccagcacc gtggcaacca caaacaagat cgcgacaaat ttggtgtaaa acgcgttcga
    19321 ctgaataccg taaaacagca ctgccagcga taacgccaca aaagggatct caaaagtcat
    19381 cgagatcaga atcaccagca ggcagcctac ccagagttgc acgatctgcg gcacgcggcc
    19441 gggtcgccgc tcgcttaact cttcatgaaa gaacgccagc agcctgacca ccggtaatgg
    19501 cagggagttg agcgcgctca ttattgcggc tcaagattag cgactgccga agcgccgatg
    19561 cggaacattt ccgggtcagg tttatcgacc atgattttga ccggaaaacg ctgggcaacg
    19621 cggacccagt taatagagcg agaaactttc ggcaggccgc ccagcaccag gccgccgtca
    19681 tccggtagca cgccgtagcc aatcgaatcc actttaccct cgaaggtttt gccgctgtca
    19741 ctcatcaggc gaatcgttgc gggtgtacct gagcgaatat ttttcagatc ggtttcgcgg
    19801 aagttggcga tcacgtacca gtgacgagtg tcgattaggg taaaaatagg gcgcatggca
    19861 gaagcaaatt gcccgacgga ggttttgagg gaaatgaccc ggccatcaaa cggcgcgcga
    19921 acggtcgcca tttccagatg aagtttggtc agggcaatat ccgcttcgac cgccgcacgt
    19981 tgcgccacca gcgcatcgac gccgctgacg gcgctggcgg ctgactgcgc ctgtaacaat
    20041 acggcattaa gatcggcttc tgcggcgcgc tgcgcagttc ttgcacggtc aacgtcttcc
    20101 gctgagacaa aaccctcttt cagtaatggc tcggtgcggc gtaatgtatc tgtggcctgt
    20161 ttcgcggcgg cacgggcttt ttctaccgtg gcattaatcg agtcggcacc aaactgttgc
    20221 gcatcaacgc tacgctgggt gagcataatt tgcttatcca gcgccgcgag ggaggcttca
    20281 gctttcgcca gattggcttc gaacgggcgc gggtcaatgc ggaacagcaa atcgccctgt
    20341 ttgactgcct ggttgtcggt gaccgccagt tctacaatgc ggccgctgac ttccggcacc
    20401 acatcaatgg tatctgctga cgcgtaagcg tcattagttg atggcgcact gtctacgcgc
    20461 cagataacga aaacaagggc aaccagcgcc aatgcaacca ctaacagagc agggaattta
    20521 ctgcgaggag cttttttcgg cgtactttcc atgagcgcgt ctcaaataga ttagaaaaat
    20581 gccagccaga acagcatggc gtagagagca aaaagggcgg tataaataat tccgacaaat
    20641 gccagattga tatttgcccg ctgaataact cgcctcgtga tgagcatcaa aataaggctg
    20701 gcaatggcgc agaaaaacca gctgggataa taagcaccga tcaccgggat tgccggagat
    20761 aatgagcatc ccgaaaccat gaagacggga attatccagg ctgttttttt gagttgcatc
    20821 gccatgcgag agagaactgt tggcatatta aatacatcct gtctgctgcg aacaatatct
    20881 gtagtgaatg tcaatatcca ttgaaggcgt cagtatggct attttctcga tgaataaaaa
    20941 tagctttatt cagccaccgc attcataact tatatatttt atttattaat tggctgtata
    21001 tatttttaaa ataaatgctt ttgggggctt cagcaccccc aaaagcgagt gacattaatt
    21061 tggggttacg atattcaccc acaaatcgaa attatccaga caggcgataa tttcttccag
    21121 tttcgcgcca ttgccaataa ttttggcttt cttcgctttt accagatccg ccattttggc
    21181 ttgcccggtc agcacggcgt gcagatcttc acggctgatg tagaaagagg cgtcagcttg
    21241 ggattgcagt gttttacggt agttaagcac gctatcgttc agcgtcaggt tgaggttatc
    21301 gccattgctc atattgaagt tcaggctgat atttttaccc gcggctttcg cgctatcgag
    21361 gcgaacggac ataaagtcga acagcatttc gaccgacatc ccgcgaatgg tgtccgggga
    21421 accggtggtg ccgtggctga acttatgcac cccttcgcgc agctctttcg cgccggtcag
    21481 gtagaaaccg cgccaggtgg cggactcggc ctgatagccc agctgttcaa agttattcgc
    21541 ttgcagattc ttcgcgacct ggtcacccgg gttggcggca atcacctgtt tcagcagttc
    21601 tgccgaccag cggtaatcac cttgcttgtt cgcttcttgc gccaggttga tgacacgggc
    21661 agaaccgccc agcgcctgca cgtaacgttt acccatctcc acctgaccat acggatgcag
    21721 gttagccggg ttaccgtcgt aatagccaag atagaagtta tacaccgcgc gggcgttgtg
    21781 gctgacagaa ccgtaatagc cacggctggc ccagttattg gcaagtgcag gcggcagttt
    21841 aatcatgtcg ccgatttcat tcatggtgta gccctggttc gccaggtgca gggtctggtc
    21901 gtgaatgtac ttgatagtat cgcgatattt accaatataa tcattgatat gcttattgcc
    21961 ccagaccggc caggtgtgcg gcataaacag gacttccgcg tcgttacccc acatatccag
    22021 cgtttcgttc agatactcgg tccacttgct ggtgtcgcgg gttttcgcgc cgcgcagagt
    22081 gtagaagttg tgcagggtat gcgtggcgtt ctcggcggta cacagggctt tcagggccgg
    22141 aatatagaag tgcatttcgg ctggcgcttc gctacctggg gtcatcagga agtcaaactc
    22201 caggccgtcg ataatcatct tctcaccggt tttggtgatg gttttgttcg gtgcgatgat
    22261 gctcgggtag cccgatgcca gcgttacgcc aagaccattg ccgatgttac cctgagggtt
    22321 atgcgccagc agcaggccgt aggagtaaag tgcacggcgg ctcataatat tacccgccag
    22381 tacgttttcg ctgatggctt cgtccataaa gccagcaggg gcgataacct gcactttacc
    22441 ggatttcaca tcggcttcag agataatgcc tttcacgccg ccatagtggt cggtatggct
    22501 gtgggtataa ataaccgcaa caatcggttt ttgcgggcga ttttggaagt aaaggtcaag
    22561 ggcggctttt gcgctcggtg gcgtcaccag cgggtcgatg acaataatcc cggtatcacc
    22621 ttcgataaag gtgatgttgg agatatcctg accgcgaacc tggtacattc tgtcggtgac
    22681 tttaaacagg ccggaaatac cgtttaactg cgactgacgc cacaggctag ggttaacggt
    22741 ttcgggcgcg ggcgcattga tatcaaattt ataatcttcc ccgcgataat atggtttgcc
    22801 attagcatcg cgcagaatac cttcatccag cagcggagcg ataaatccac gctgagcgtc
    22861 gtcaaaatcc tgacgatcgc taaacggtaa tgatttggcg taattctgat taacctgttg
    22921 ggtatattga gtggcggctt tcgcttcatc tgttgcgtaa gcggtattta ccatcatgcc
    22981 gctggcggca gttaacgcaa taacaattct gctcaaacgg aataaccgag agttattcat
    23041 ataaatctcc atttatcatt tatgtcgtaa atatgtaatc catgcaaaat acgtcattta
    23101 aagctggggt tatcataatt gggaatttgc gactttaata agtggaagtg tgagcggaac
    23161 gatccatttt attaggcatt tgtgctgaag ggggaaaatt acgcgaattt attatctctg
    23221 taaataatat gaggtgtttt atatatttat tatatatatt gtacttcggc aaatactgag
    23281 cacaatttat caatgcaacc ggtgtttctt tttaaaaaac gtaccggagc aatgggcggt
    23341 catgtcgctg gcaccgcagg attctgcgtt gctgtgccag ccttttattc atggtcaatg
    23401 agtgaggtga ttgataaaga caccaggtcg tcagagcgca ctgattatcc gcattatgcc
    23461 agtgaagttg cactggcttt tggttcacga gtggttggcc tcgaactggc aaaaatgatt
    23521 gtggatgcgt ggctgggcgc acagtacgaa ggcggtcgtc atcaacaacg cgtggaggcg
    23581 attacggcaa tagagcagcg gagaaattga gattcatcca ctacttgcat ggatgagtaa
    23641 tgactaatgt ggatagagtt tctttttgag gttggctaat gaaacgcttt
//
LOCUS       DAZFVO020000073        23416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFVO020000073 DAZFVO020000000
VERSION     DAZFVO020000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.073"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            14..1063
                     /gene="yeiH"
                     /locus_tag="ACHKNB_RS13185"
                     /old_locus_tag="pgaptmp_002637"
     CDS             14..1063
                     /gene="yeiH"
                     /locus_tag="ACHKNB_RS13185"
                     /old_locus_tag="pgaptmp_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGU4196125.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1137..1994
                     /gene="nfo"
                     /locus_tag="ACHKNB_RS13190"
                     /old_locus_tag="pgaptmp_002638"
     CDS             1137..1994
                     /gene="nfo"
                     /locus_tag="ACHKNB_RS13190"
                     /old_locus_tag="pgaptmp_002638"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HGU4196126.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1998..3086
                     /locus_tag="ACHKNB_RS13195"
                     /old_locus_tag="pgaptmp_002639"
     CDS             1998..3086
                     /locus_tag="ACHKNB_RS13195"
                     /old_locus_tag="pgaptmp_002639"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGU4196127.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3142..4392)
                     /gene="nupX"
                     /locus_tag="ACHKNB_RS13200"
                     /old_locus_tag="pgaptmp_002640"
     CDS             complement(3142..4392)
                     /gene="nupX"
                     /locus_tag="ACHKNB_RS13200"
                     /old_locus_tag="pgaptmp_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4196128.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGSLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4492..5433)
                     /gene="rihB"
                     /locus_tag="ACHKNB_RS13205"
                     /old_locus_tag="pgaptmp_002641"
     CDS             complement(4492..5433)
                     /gene="rihB"
                     /locus_tag="ACHKNB_RS13205"
                     /old_locus_tag="pgaptmp_002641"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HGU4196129.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5563..6261
                     /gene="yeiL"
                     /locus_tag="ACHKNB_RS13210"
                     /old_locus_tag="pgaptmp_002642"
     CDS             5563..6261
                     /gene="yeiL"
                     /locus_tag="ACHKNB_RS13210"
                     /old_locus_tag="pgaptmp_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HGU4196130.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6332..7582)
                     /gene="psuT"
                     /locus_tag="ACHKNB_RS13215"
                     /old_locus_tag="pgaptmp_002643"
     CDS             complement(6332..7582)
                     /gene="psuT"
                     /locus_tag="ACHKNB_RS13215"
                     /old_locus_tag="pgaptmp_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HGU4196131.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7676..8614)
                     /gene="psuG"
                     /locus_tag="ACHKNB_RS13220"
                     /old_locus_tag="pgaptmp_002644"
     CDS             complement(7676..8614)
                     /gene="psuG"
                     /locus_tag="ACHKNB_RS13220"
                     /old_locus_tag="pgaptmp_002644"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HGU4196132.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8602..9543)
                     /gene="psuK"
                     /locus_tag="ACHKNB_RS13225"
                     /old_locus_tag="pgaptmp_002645"
     CDS             complement(8602..9543)
                     /gene="psuK"
                     /locus_tag="ACHKNB_RS13225"
                     /old_locus_tag="pgaptmp_002645"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HGU4196133.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10000..11691)
                     /gene="fruA"
                     /locus_tag="ACHKNB_RS13230"
                     /old_locus_tag="pgaptmp_002646"
     CDS             complement(10000..11691)
                     /gene="fruA"
                     /locus_tag="ACHKNB_RS13230"
                     /old_locus_tag="pgaptmp_002646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HGU4196134.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWQGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVDASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11708..12646)
                     /gene="fruK"
                     /locus_tag="ACHKNB_RS13235"
                     /old_locus_tag="pgaptmp_002647"
     CDS             complement(11708..12646)
                     /gene="fruK"
                     /locus_tag="ACHKNB_RS13235"
                     /old_locus_tag="pgaptmp_002647"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGU4196135.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12646..13776)
                     /gene="fruB"
                     /locus_tag="ACHKNB_RS13240"
                     /old_locus_tag="pgaptmp_002648"
     CDS             complement(12646..13776)
                     /gene="fruB"
                     /locus_tag="ACHKNB_RS13240"
                     /old_locus_tag="pgaptmp_002648"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HGU4196136.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14144..15325
                     /gene="setB"
                     /locus_tag="ACHKNB_RS13245"
                     /old_locus_tag="pgaptmp_002649"
     CDS             14144..15325
                     /gene="setB"
                     /locus_tag="ACHKNB_RS13245"
                     /old_locus_tag="pgaptmp_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HGU4196137.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15322..15576)
                     /gene="yeiW"
                     /locus_tag="ACHKNB_RS13250"
                     /old_locus_tag="pgaptmp_002650"
     CDS             complement(15322..15576)
                     /gene="yeiW"
                     /locus_tag="ACHKNB_RS13250"
                     /old_locus_tag="pgaptmp_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4196138.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15731..16303
                     /gene="yeiP"
                     /locus_tag="ACHKNB_RS13255"
                     /old_locus_tag="pgaptmp_002651"
     CDS             15731..16303
                     /gene="yeiP"
                     /locus_tag="ACHKNB_RS13255"
                     /old_locus_tag="pgaptmp_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HGU4196139.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16526..17992
                     /gene="yeiQ"
                     /locus_tag="ACHKNB_RS13260"
                     /old_locus_tag="pgaptmp_002652"
     CDS             16526..17992
                     /gene="yeiQ"
                     /locus_tag="ACHKNB_RS13260"
                     /old_locus_tag="pgaptmp_002652"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4196140.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18110..19096
                     /gene="yeiR"
                     /locus_tag="ACHKNB_RS13265"
                     /old_locus_tag="pgaptmp_002653"
     CDS             18110..19096
                     /gene="yeiR"
                     /locus_tag="ACHKNB_RS13265"
                     /old_locus_tag="pgaptmp_002653"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HGU4196141.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            19135..19848
                     /gene="lpxT"
                     /locus_tag="ACHKNB_RS13270"
                     /old_locus_tag="pgaptmp_002654"
     CDS             19135..19848
                     /gene="lpxT"
                     /locus_tag="ACHKNB_RS13270"
                     /old_locus_tag="pgaptmp_002654"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HGU4196142.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVTGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20260..20826
                     /gene="mepS"
                     /locus_tag="ACHKNB_RS13275"
                     /old_locus_tag="pgaptmp_002655"
     CDS             20260..20826
                     /gene="mepS"
                     /locus_tag="ACHKNB_RS13275"
                     /old_locus_tag="pgaptmp_002655"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HGU4196143.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21007..22563
                     /gene="pdeN"
                     /locus_tag="ACHKNB_RS13280"
                     /old_locus_tag="pgaptmp_002656"
     CDS             21007..22563
                     /gene="pdeN"
                     /locus_tag="ACHKNB_RS13280"
                     /old_locus_tag="pgaptmp_002656"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HGU4196144.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22645..>23416
                     /locus_tag="ACHKNB_RS13285"
                     /old_locus_tag="pgaptmp_002657"
     CDS             22645..>23416
                     /locus_tag="ACHKNB_RS13285"
                     /old_locus_tag="pgaptmp_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4196145.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDD"
BASE COUNT         5801 a         6078 c         5643 g         5894 t
ORIGIN      
        1 aaaggagagg gttatgacga atatcacctt acagaaacaa catcgtacac tgtggcattt
       61 tattccgggg ttagccctga gtgcagttat caccggggtc gccctgtggg gtggttccat
      121 tcccgcggtt gcgggtgccg ggtttagtgc cctcaccctc gcaatcttgt tggggatggt
      181 tttaggcaac accatctatc cgcacatctg gaaaagctgt gacggtggtg tgctgtttgc
      241 caagcaatat ttattacgtc tgggtatcat tctttatggc ttccgtctga cgttctcgca
      301 aattgccgat gtcggtatca gtgggatcat cattgacgtg ttgacgctgt ccagtacctt
      361 cctgctggct tgcttcctgg ggcagaaagt gtttggtctg gataagcaca ccagctggtt
      421 gatcggtgcc ggtagcagta tctgtggtgc tgccgcggta ctggcgactg agccggtagt
      481 gaaagcggaa gccagtaaag taaccgtggc tgttgcaacc gttgttatct tcgggaccgt
      541 cgcgattttc ctctacccgg cgatatatcc gctgatgtct caatggttta gtccggaaac
      601 tttcggtatc tacattggtt ctactgtgca cgaagtggcg caggtggtgg cggcaggtca
      661 tgccatcagc ccggatgcgg aaaacgcagc agttatttcc aaaatgctgc gcgtgatgat
      721 gctggctccg ttcctcatcc tgctggcggc gcgtgttaaa cagctgtctg gggcgaacag
      781 cggcgagaaa agcaaaatca ctattccgtg gtttgccatc ttgttcatcg tagttgccat
      841 ctttaactcg ttccacctgt taccgcagag cgtggtgaac atgctggtaa cgctggatac
      901 cttcctgctg gcaatggcga tggcggcgct gggtctgacc actcacgtca gcgcgctgaa
      961 aaaagccggg gcgaaacctc tgctgatggc actggtactg tttgcctggc tgattgttgg
     1021 tggtggtgcg attaactatg tgattcaaag cgtcatcgca taaaccacta catcttgctc
     1081 ctgttaaccc gctatcatta ccgttttcct ccagcgggtt taacaggagt cctcgcatga
     1141 aatacattgg agcgcacgtt agtgctgctg gcggtctggc aaatgccgca attcgcgccg
     1201 ccgaaatcga cgcaaccgcg tttgccttgt tcaccaaaaa ccaacgtcag tggcgtgccg
     1261 caccgctcac gacgcaaacc atcgatgaat tcaaagcggc ctgtgaaaaa tatcactaca
     1321 catcggcgca aattcttccc cacgacagct atctgattaa cctcggacat ccggtcactg
     1381 aagctctgga aaaatcgcgc gatgccttta tagatgaaat gcagcgttgc gaacaactgg
     1441 ggctttcttt gctcaacttc caccctggca gccatctgat gcagatttca gaagaggatt
     1501 gccttgcgcg tattgccgaa tccatcaaca ttgcgctgga taaaactcaa ggtgtaacag
     1561 cggtgattga aaacaccgcc ggtcagggca gtaacttagg gtttaaattc gaacatctcg
     1621 cggcgattat cgacggcgtg gaagataaat cccgtgtcgg cgtctgcatt gatacctgcc
     1681 atgctttcgc tgccgggtat gatttgcgta ctccagccga atgcgaaaaa acgttcgctg
     1741 attttgcccg tattgtcggc tttaagtatc tgcgcgggat gcaccttaac gatgcgaaaa
     1801 gtacctttgg cagccgcgtt gaccgccatc atagcctcgg tgaaggcaat atcggtcatg
     1861 atgcgttccg ctggatcatg caagacgacc gtttcgacgg cattccgctg atccttgaaa
     1921 ccatcaaccc ggatatctgg gcagaagaga tcgcctggct gaaagcgcaa caaactgaaa
     1981 aagcggtggc ctgatcgatg aacgacaggg aaaagcagat tcttaaaatt ttacggcgta
     2041 acccgctgat tcaacagaat gaaatcgccg atattctgca aatcagccgc tcacgtgttg
     2101 ccgctcatat tatggatttg atgcgcaagg gtttgattaa aggcaaagga tatatcctta
     2161 ccgagcagga ttactgtgtg gttgtggggg caatcaatat ggatattcgc ggaatggcgg
     2221 atatccgtta cccgcaagca gcctctcatc caggttctgt tcactgttcc gcaggcggcg
     2281 tcggacgtaa tatcgcgcat aacctggctc tgcttggtcg ggatgtgcat ttaatatcag
     2341 ctattggcaa cgatttttac ggcgagacat tactggaaga aacgcgccgc gcgggcgtga
     2401 acgtttcaaa ctgcattcgt ctgcatggac acagcactgc aacttatctc gctatcgcta
     2461 ataagcagga agaaactatc ctggcaatca atgacacaca tattctgcaa cagctaacgc
     2521 ctcaactgct caatacttca cgggacttga ttcgccatgc tggtgtggtg ctggctgatt
     2581 gtaatctcac acctgaagcc ctggagtggg tctttaccat tgctgatgaa attccgatgt
     2641 ttgtcgatac cgtttccgaa tttaaagcaa acaaagtaaa aaactggtac agccggatcc
     2701 acactctgaa accaacacaa aatgaactgg aaattttgtg ggggcaaccg attaaagacg
     2761 acaacgatcg catcagagct gtaaattcgc tgcatcaaca aggtgttaaa cgcatttttg
     2821 tttatctgaa agatgaatct gtattttgca gcgataagga tggagaacaa tttttgctga
     2881 ccgcgccagc gcatacgaca gtagacagtt ttggtgctga cgatggtttt atggcgggcc
     2941 tggtatatag ctttcttgaa ggatacagtt tccgcgacag cgcccgtttt gcggtagcct
     3001 gcgcggcaat ttcgcgcgcc agcggcagct taaacaaccc taccctgtct gccgataacg
     3061 cgctttcatt agtgccaatg gtgtaacaat gttgccggat tcgacgcttg accgcatctt
     3121 atccggctca taaattgcgc actacgctaa accaataaag aatcctgcaa tagtagcact
     3181 catcaggtta gaaagtgtcg ccgccgccag cgcgcgtaaa ccaagttggg cgatttccgg
     3241 cgcacggtgt ggcgcaaccg cagaaaacgc ccccaccacc accccgatag aaccaaagtt
     3301 agcgaaaccg cacaacgcga aagaaataat cgccacggtt ttagcatcca gagagccacc
     3361 cgtttgcaga tatggcgaga aattgagata agcgacaaat tcattgatcg ccagcttctg
     3421 cccaatcaaa ctcccggcaa gatttgcatc actccagtca acccccatca cccacgccaa
     3481 tggggccaac aggtaaccta aaatggactc cagcgaggca tgtgcaaaac caaaccagcc
     3541 gccaacgccg ccgataatac cattaattaa cgcaatgatg gcgacaaatg ccataacaac
     3601 tgtcgctaca ccggcggcga ttttcagccc ggtcattgcc cctgtcgcag cggcttcaat
     3661 aatgcttttt ggcggtgttt cggtgaaaga gaggttatta aaggaaacct gcgaagattc
     3721 cgtagccggg cttaacaggc gggcaaacaa gatcccgcct gggatcgcca ttaacgatgc
     3781 cgccagcaaa tattccacag gtacgcccag ggcggcgtaa ccaatcattg tcgaaccagc
     3841 aatcgaggcc atgccactac aaatcgctgt aaataattca ttgcgattca gacgatcgat
     3901 aaagggtttg acgatcgccg gaatttcgtt ttgcccgagg aaaatggtgg tgaccgcgac
     3961 gaatgactca atcttgctga tatttaatgc tttctggaat ataccgccga gaatgcgaat
     4021 taaaatcccc atcacaccga tgtagtagag aatactcacc agtgcagtga cgaagataat
     4081 tgccggtaat accctgaaac caaagataaa tcctgcgcca tcaaacagcg tgtccatttt
     4141 cggcccgacc agcgaaccga agataaatgc gctacccgcg tcgctgtacg ccatcacttt
     4201 atgcacgcca aaagcgactt tttcagcgac ccaacgccct ggcggtaacc aaagcataat
     4261 cccgccaatc acgacctgta acactaacgc cgcgccaacg gtacgcaggc tgatcttctt
     4321 cttgtttact gacagcaaaa acgcaatcgt cagcaatacc accattccca gaacacttct
     4381 catgacatcc atagttatta tctcttcatg tcatattggg cgacggcttt ctgcccatca
     4441 gaaatagccg ggcttggatc ttgcgatact gacccggctt gttatcaaca cttaatgggt
     4501 tttgatgtag ccgcgcacgc actcttcgac taagccccag aaccagtcgg tatcaatagt
     4561 gatgccgact ttggtattag cgggcttgcc aagaacgccc agctcgtcgc agacggtacg
     4621 cccatagcaa gggccactgt tgacgtccac ttcgacgtac atctcctgtg ttttaatgcc
     4681 atcagggtta atcagataac cgatgcaggt ggcgtcgtgc accgggccgc cagccaggcc
     4741 gtagttttcg aactgcgttt tgagagtgaa gttcatgatg tcgctgaaca gctctccggc
     4801 agggccgcct gccctttcca tccgtgcaat cacgtccggg gtgcaaacgg tctggttggt
     4861 gagatcgagg cccatcatca ctaatggaac gccggaggtg aacaccacgc gggctgcttc
     4921 cggatcagca aagatgttga actcggcaga tggcgtgaag ttgcctgtac cgtaagcgcc
     4981 gcccattagc acaatttcac ggattttggg caggatcgcg ggttgcatac gcattgccac
     5041 cgcgatattt gaaagcggac caaccggcac cagagtgata tcgccatcgc ttgccatcag
     5101 ggtatcaatg atgtatttca ccgcgtgggt actttctgcc tggcgggtca gcggctcgaa
     5161 taccgggcca tccagtccgg tttcaccgtg aatattatcg gcaacgattt gtttacgcat
     5221 aatgggttgc ggcatccccg cataaaccgg aacattaatc tccagtttct ggcaaacatt
     5281 caggccatta attaatgttt tatcaagcgt ctgattaccc gctacaatag tgatgcctaa
     5341 taaatctatt gccggatgtt tcgctgccat catcattgca atagcatcat catgacccgg
     5401 atcacaatcc agaataattt ttctcttttc cattgtttat ttcctctgtt tccagttgcg
     5461 ttattttttc tacagcaaag aaaattcgac cgggccgatg attgaatctt accaaccgcg
     5521 tacgtatgct aaatatgaga aatctcatag cggataaaca tcgtgaaaga aatccacaat
     5581 aatgatctta agcagcaatt gatgagtgaa tccgcgttta aggattgctt tttaacggat
     5641 gtttcagccg atacgcggct gtttcatttt ttagcgcgtg actacattgt gcaggaaggg
     5701 caacagccgt cctggctgtt ttacctgacg cgaggccgcg ccaggcttta cgccacgcta
     5761 gctaatggtc gcgtgtcgct gatcgatttc tttgccgccc cctgttttat tggcgagatt
     5821 gagttaatcg acaaagacca tgaaccgcgt gcggtgcagg ctattgaaga gtgttggtgc
     5881 cttgcgctcc ctatgaaaca ttaccgcccg ctgttattaa acgacacgct atttttacga
     5941 aaactctgcg tcaccttaag tcataaaaat tatcgtaata ttgtttcttt aactcagaat
     6001 caatcatttc cgttagttaa tcgcctggca gcttttatat tactctcgca ggaaggtgat
     6061 ctttatcacg aaaagcatac gcaagcggca gagtatttag gcgtttctta tcgacatctt
     6121 ttatatgttc tcgcgcaatt cattcacgac ggtttattaa ctaaaagcaa gaaagggtat
     6181 ctcattaaaa acagaaagca gttgtcagga ctggcgctgg agatggaccc ggagaataaa
     6241 ttctccggga tgatgcagta aaaattattt gcaatagcgc gattgccgga tgcaacgctt
     6301 aatacgtttt atccggtcta caaatcgagc attacgccag accaataaag aacccggcaa
     6361 tagtcgcact catcaggttg gaaagcgttg ctgctgccag cgcccgtaaa ccaagctggg
     6421 cgatttctgg agcacgtttt ggcgaaatag ccgaaaatgc gccaacgaca acaccgatag
     6481 aaccaaagtt tgcaaaccca cagagggcga acgagataat ggcaatggtc ttgacatcca
     6541 gcgtgccgct cgtttgcaga tagggcgaga aattgagata agcaacaaac tcattaatcg
     6601 ccagtttctg cccaatcaaa cttccggcaa gatttgcatc actccagtcc acccccataa
     6661 tccatgccaa cggggccagc acatagccga aaatgctttc cagagagaca ttggcaaaac
     6721 caaaccagcc gccaactcca ccaataatgc cgttgatcag cgcaataatt gccacaaatg
     6781 ccattaccac cgtcgccaca ccggcggcga ttttcagccc ggtcattgca ccgttcgccg
     6841 cagcttcaat aatgcttttt ggcggtgttt cgctaaacga cagattttca aacgtaactt
     6901 gcgaaggttc cgtcgccgga ctaagaatac gtgcaaacag gataccgcca ggaatagcca
     6961 tcagcgaagc ggctaacaaa tagtcaatcg gcacgcccat tccggcatag ccaatcatca
     7021 tcgaacccgc aatagacgcc atgccgctac aaatagccgt aaatagctcg ttgcgattca
     7081 tgcgatcaat aaaaggttta acgatcgccg ggatctcatt ttgcccgagg aaaatagtgg
     7141 tgactgcgac aaaagattcg attttgctga tgttgagggc tttctgaaaa atactgccaa
     7201 gaatacgaat cagcagcccc attacgccaa tgtagtacag caagctgata agcgcggtaa
     7261 cgaaaataat cgccgggagt acgcgaaagg cgaagataaa gcccgcaccg tcaaacagaa
     7321 catccatttt gggaccgacc agcgaaccaa aaataaaggc acttcctgcg tcactgtagg
     7381 acatcacttt atggacgcct aatgccgcct gctctactgc ccattttccc gggggaaaat
     7441 agagcatgat gccaccaatg gcaatttgca gcagcagcgc agcgccaacc gtgcgcaagc
     7501 tgatgctctt tttatttacc gacaacacaa aggcaattgc cagcaatacc accatcccca
     7561 caacacttct cattatatcc ataatgattt tcccttcatg ccagtaaacc cggcgtcagc
     7621 gccaggtttt ggtatgtttg atgagtacgg gcgacggctt tctgcccgcc agaaattaac
     7681 ccacgagacg ctgatattct ttggcaattt cgctcgccag aatggcgtta ttaaagacca
     7741 gttgaatatt ggatttcagg ctatcaccgc ctgttagctc cgcaacacgc gccagcagga
     7801 acggtgtact ctctttccca ataacaccct gttcttcggc ttcagcaacg gcttgatcaa
     7861 ttgctgcatt gatggtttct tctggcatcg caaactgttc cgggatcgga ttcgccacta
     7921 ccaggccgcc gttcaaaccg gtttgccact tcactgccat cgcgcgagca atttcgcggg
     7981 cactgtcaag acgaatgctg acttcaaatg ggctggttcg gcagaaaaac gcaggcagcg
     8041 cttttgtctg ataaccaatt aacggtacac cgaaggtttc taaatattct gttgttaatc
     8101 ctaaatcgag aatagatttt gcccccgcac aaacaacagt gacattggta tttgccagtt
     8161 cctgtaaatc ggcagaaata tcgaaggtat gttccgcccc gcgatgcaca ccaccaattc
     8221 ccccggtggc aaatacttta attccggcaa gcgccgcaat aatcatcgtt gaagccacag
     8281 tggttgcgcc attttttccg gcggcaacaa caaaaggtaa atcgcgacga ctaactttgg
     8341 tcacgttatg tccttcacgg cccagcaatt cgatttcttc tttacttaag cccactttca
     8401 tcacaccgcc aataatggcg atagtggctg gtactgcgcc ctgtttacga atagtttctt
     8461 ctacttcaat tgcggtttgg gcattttgtg ggaatggcat tccgtgagaa attatggtcg
     8521 attccagcgc aacaaccggt tttttatttt ttaaagcatc ctgtacttca gcggatattt
     8581 gtaataattc aggggacaga gttaattcag acattctgtg ttctccacta acgatctaac
     8641 attggcaatt gataattcag ggttgttggt atattcacag gcaagtgcca ttgacgaaca
     8701 tccctgcgca aatcgaacag aatcgataaa cggcataccg tctacccaac aggaggccag
     8761 ccccgccatc atggcgtcgc ctgccccagt aacattgatg acattcgttt taataggggc
     8821 cgaccaccca ctttcaccgt tgatatcact gtagtaaacg ccatcccctc ccatgctcag
     8881 cactaggcgg ttcaggccgt gttgatgaaa ccaggcggcg acctttgcga catcttcacg
     8941 tccggaaagt gcaattccgc tcaacgtttc ggcttcaaga cggttaggtt ttaacgtatg
     9001 gattttgctc agatgatcgc ggattttgac gcatttccat gccgataccg gatcaataaa
     9061 tacaggcgtt tccccgctat tttccagaac ccagaccagc gcctcttcac taaggttgca
     9121 atccgcgaca ataacctttg caccgcgaat aaactcctga tgctgggcaa gaaattctgc
     9181 agaaatacag tcactaatac tcatatcatt tatcgcgaca agcatttctc ctgtattatc
     9241 gagcagtgat aaatagctgg atgtattctc acccggaaca attaagcatt tatcgacata
     9301 aacaccggat tgattagttt gcgccagtaa tgattgccca taaaaatcac cacctactgc
     9361 acttaaaagc caggcatttt tccccagtaa cgccagatta tgagcgatat tccttcccac
     9421 gccacctggg gtaaatttaa tttttccggg attcgaatcc gcataattca atggatgatg
     9481 tgaataccca gccacatcaa tattcgccga accaataatt actatgtagt ccttttcgcg
     9541 catagcacgc cccctggcaa taaattgcca acgaagaaat tgggtaaaat agtcattaca
     9601 gtcaataaga ctattagagc acgtgttcat atttaaacac atgttcataa taagcatatt
     9661 taacgtaatg taaatcgatc agaaaaaacg aaatcggcca gatatgaagg cggtcacaaa
     9721 gtttgtctat ttatgcagca acaaaagaga aagttgtgat gtgtaacaca caatatggca
     9781 ggcaagaaaa agggcagaga tgtttctgcc ctccagattg ttgacaacgc gcgctttgtt
     9841 catcctggat gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa ttcaatatat
     9901 tgcagggttc atgtaggtct gataagcata gagcatcagg caattttgcg tttgtcatca
     9961 tcggggcaga gatgtctcat ccccgataat gaagataaat tatgcgtttt tttctactat
    10021 ttgtgtttcc gggcgtttca ggaaggcata ggccagaccc gccaccagcg taccggcaat
    10081 aatggctacc aggtaaccca gtaccggcgt aatcgcgcca gggatcagca gaacaaacag
    10141 accaccgtgc ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc
    10201 cccacccacg atacagcacg gcagcacacg catcggatca cgagcagcaa acggaattgc
    10261 accttccgaa atgaagcaca gtcccaatac cagagcggct ttgccacctt cctgctgcgc
    10321 tttgtcgaat ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat
    10381 acctgccgcc ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc
    10441 gaacgcgtac gctgctttat ttaccggacc gcccatgtca gtacacatca tgccaccgag
    10501 gatcgccccc agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcagccc
    10561 ttcgagaatg ccagcaactg gtttaccgat caggtagatc atcgccagac cgaccaccag
    10621 actggaaatt agcggaatga tcaggattgg tttcagcgcc tccatactct gtggcagttt
    10681 cagttgcgta ctgattaact tcgcaatgta accagccagg aagcccgcaa taataccgcc
    10741 aatgaagcca gaaccggtgc tgacagccag cataccgcca atcagacccg gcgtgaggcc
    10801 cggacgatcg gcaatggaga aggcaatata acctgccagt accggcacca tcagcgcaaa
    10861 ggctgaacca ccgccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc
    10921 ttcgatacca aaagcaaaag aaagcgcgat acacagacca ccagcaacga ccatcggcag
    10981 catgtaagaa acgcccgtca gcaagtgacg gtaagcgccc gcactctctt tcttaccttc
    11041 agtggtcgcc gtttgagctt tgcccgccgg ttcatacggc gttgcttcag caaccgcttt
    11101 atccagttcc tgcgcggttt tcttcagcgc cagaccggta gaggtgcgat acatcggttt
    11161 accagcaaat ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc
    11221 ggctacttct tcgggagtga ttgcattacc cgcgccaaca gaaccacggg tttcaacttt
    11281 cacccaccag ccacgttttt tcgcttcggt ttcaatggct tcagcagcca taaaggtgtg
    11341 tgctacgcca gtcgggcaag cagtcaccgc aactacgcgt ttcgggccgc tggcgtcaac
    11401 cggtgctgtc gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag
    11461 gaacagctca gggtgcgcaa ctgcccggga aatatcgccc tgccagacat ttttaccgtt
    11521 cagcgcgctg tcgttcggga tggaatcacc gagaacaatc gccatttcag cgtcgttcgg
    11581 attgtcgatg atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat
    11641 ataggcgcgt gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct
    11701 cctgctgtca gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac
    11761 gatcggtaat acccacattg ctttgactta ccgccagggc tgcaacagca gtcgccagac
    11821 gcagtgtgtg ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag
    11881 aatcccctgc gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc
    11941 cggaggcatt aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac
    12001 cttgttcacg tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc
    12061 ctgcccagat ttccagctcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac
    12121 ctgctactaa cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc
    12181 gagtcatcca gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac
    12241 agaccatatc gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg
    12301 cgggggtgac ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca
    12361 gcttaacgtt aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct
    12421 cgctgaacag ttgctgaaaa ccatcctgat tgtctttacc gaggaagccg ccaacggtga
    12481 catcaattcc caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca
    12541 gaccggtggt tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa
    12601 ggtcataagc cggattaagg gtgatcgtag caacacgtct gctcattatg cgccctcccc
    12661 aagaccagca gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg
    12721 ggcggtaaag cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact
    12781 acgtccgttt gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa
    12841 ttgtttaatg gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg
    12901 caccacaaac tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc
    12961 cagcaacgtt gccgcatccg ctttcagcaa gcgatcagct ttattgtcga gcaacaaatc
    13021 agcgagacgc ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat
    13081 ggctgccgtt tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct
    13141 acgcagattg ccttcggcgc tatcgctcag ccagataccc tgtccgaggt tcagcggttg
    13201 ttcattgatg gctttggtga cgaaagtggc gtcaactgcc cccgcctctt tcagacgcgc
    13261 agcgttcagc gcctgaagag tcagcagatc gctggcgacg atatccagcg tcagcatttc
    13321 gttgtcgagc ttcagctgct cactctgctt ttcgcccatc agtaatgcgc gaagttcttc
    13381 tgctgttgtt gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag
    13441 ctggcgtagc aggcccagat gttcatccga gctggcagca ataccgattg ccacgtacgc
    13501 tacctgaccg tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt
    13561 cagcacctga tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa
    13621 cgttgaggtt tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacattacc
    13681 ggcctgcacc agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc
    13741 gcccggatgg atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga
    13801 aacgattcag cctctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc
    13861 gcgctgaaac gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa
    13921 agttatgagc aaaagtttga tgtgctgcac aattttttgc catttttcct aattgctgat
    13981 gggaaaatcc gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg
    14041 aaatggcatt ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa
    14101 gcagcgtaaa ctccgcgtct tcctcttcca gtgatcgacc agcatgcata actcccccgc
    14161 agtctccagc gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg ttgcctttct
    14221 caccggtatt gcgggcgctc tgcaaacccc gacactcagt atttttctta ctgatgaagt
    14281 acatgctcgt ccggcgatgg tgggattctt ctttaccggc agcgctgtca ttgggattct
    14341 ggtaagtcag tttctcgccg ggcgctctga taagcgcggc gatcgcaaat cgctgattgt
    14401 cttttgctgc ctgttaggcg tgctggcctg cacccttttt gcctggaatc gcaactactt
    14461 tgttttgcta ttcgttggcg tctttcttag cagctttggc tcgaccgcta acccgcaaat
    14521 gtttgccctt gcccgtgaac atgccgacaa aaccggacgt gaggcggtga tgttcagctc
    14581 ttttttacgc gctcaggttt cactggcatg ggtcattggc ccaccgctgg cttatgcctt
    14641 agcgatgggt ttcagcttta cggtaatgta tctgagcgca gcggtagcgt ttattgtttg
    14701 cggtgtgatg gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg
    14761 cacgatcgaa gcgccgcgcc gtaaccgtcg cgatacgctg ctgctgtttg tcatttgtac
    14821 attgatgtgg ggctcgaaca gcctgtacat catcaacatg ccgctattta ttatcaacga
    14881 actgcatctt cccgagaaac tggccggtgt gatgatgggg accgccgccg ggctggaaat
    14941 cccgacgatg ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg
    15001 cgttgctgcc gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc attcacctgt
    15061 cattctgttg ggcttgcagc tgctaaatgc tatttttatt ggcattctgg gcggcatcgg
    15121 gatgctctat tttcaggatc tgatgcccgg tcaggcgggt tcagccacca cgctctatac
    15181 caacacgtcg cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg tcgccgagat
    15241 ctggaattat cacgctgtgt tctggtttgc gatggtgatg attatcgcca ctctgttttg
    15301 cttactgcgg attaaagatg tttaaggcgc ggtcagcatt tccagatcga taagccaggt
    15361 catggcctgc tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc ctgcacagac
    15421 tttcggtcgc agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca
    15481 gggcgtattg gcgggcttgc catctggcat accgggaata gggctggaaa ttgaaggggc
    15541 agtgcaacac gccccgcaac ccggacggca tttcatacgt tgttctctta aggttaacca
    15601 ccggattgat gagcgcgcac agtaccacct tttttgcacc agcaaaagtg cgaataccac
    15661 ttgcccgaaa ggcccgtcgc gagtactttg tcgcgatatt tttgacattt tcgactacag
    15721 gaatttttcg atgccaagag cgaacgaaat taaaaaaggt atggtactga attacaacgg
    15781 caaactgctg ctggttaagg atattgatat tcagtcgccc actgcccgcg gtgccgctac
    15841 gctgtacaaa atgcgttttt ctgatgtccg taccgggctg aaagtagaag agcgtttcaa
    15901 aggtgatgat atcgttgaca ccgtgacgct gacccgccgt tacgttgact tctcctatgt
    15961 cgatggcaac gaatatgtct ttatggataa agaagactat accccgtata ccttcaccaa
    16021 agatcagatt gaagaagagt tgctgtttat gccagagggc ggcatgccgg acatgcaggt
    16081 gctgacctgg gacggtcaac tgctggcgct tgagctgccg cagaccgttg atctggaaat
    16141 cgttgaaacc gcaccaggca tcaaaggggc atctgccagc gcccgtaaca aaccggcgac
    16201 attgagcact ggtctggtga ttcaggtacc agaatactta agcccgggcg aaaaaattcg
    16261 tatccatatc gaagaacgcc gttatatggg gcgtgctgac taacttcagc cgcatgcaga
    16321 aaagggatag ctcaggctgt ccctttttta atttattata ccaatcttct attttgcgct
    16381 tcctgaacac cttacgccct caattttcac tcgttgattg atcgccctca cacttcatcg
    16441 cattaacaat ccagaccaat ttcaattgct gtcatataac tttacactgt cattgttaat
    16501 ttatcgttac taagacgtga ctcctatgaa gacaattgcc tccgttacgc tcccgcatca
    16561 tgtacacgct ccacgctacg atcgccagca gttgcaatca cgtatcgttc attttggctt
    16621 tggcgccttt caccgcgctc atcaggcgtt actgaccgat cgtgtgctga atgcccaggg
    16681 cggcgactgg gggatctgtg aaatcagctt gttcagcggt gatcaactga tgagccagct
    16741 ccgcgcacag aaccatttat ataccgtgct ggaaaaaggt gcggacggca atcaggtgat
    16801 aattgtcggt gccgttcacg aatgccttaa tgcaaaactg gattccttag cggcaattat
    16861 tgagaaattt tgcgagccac aggtggcaat tgtttccctg acgattaccg aaaaaggcta
    16921 ttgtattgac ccggccaccg gtgcactcga caccagtaat ccgcggatta ttcacgatct
    16981 acaaacccct gaagaacctc actccgcacc gggtattctc gtcgaagcac tgaaacgccg
    17041 ccgtgagcgc ggccttacac cgtttaccgt gctctcctgc gacaatattc ccgacaatgg
    17101 ccatgtggtg aaaaacgcgg tgctgggaat ggcagaaaaa cgttcgccag aactcgccgg
    17161 gtggataaaa gaacacgtca gttttccggg aaccatggtc gaccgcattg ttccggctgc
    17221 aaccgacgaa tcactggcag aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat
    17281 tagctgcgaa ccgtttatcc agtgggtggt ggaagataac ttcgtcgctg ggcgtcctgc
    17341 ctgggaagtc gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag agatgaaact
    17401 gcgtatgctt aatggcagcc actcttttct cgcttatctg ggttaccttt caggattcgc
    17461 ccatatcagt gattgcatgc aggatcgcgc atttcgccat gccgccagaa cattaatgct
    17521 gaatgagcaa gcgccgacac tacaaattaa agatgtcgat ttaacacaat atgcggacaa
    17581 gttaattgca cgttttgcta atccggcgct gaaacataag acctggcaaa tcgcaatgga
    17641 tggtagccag aaattaccgc aacgtatgct ggcaggtatt cgcatacatc tggggtgcga
    17701 aacggactgg tcgttgctgg cattaggcgt tgctggctgg atgcgttacg tcagcggcgt
    17761 tgatgatgcc ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact
    17821 tgttgcgggc agcagcagtg aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt
    17881 cggtgatgat ctgccagata acccgcattt tgtgcaggcc atcgaacaag cctggcaaca
    17941 aatcgtacaa ttcggcgcac atcaggcgct attaaacacc ctcaaaattt aacgatttct
    18001 gcggttaaag cggatgaagc tcaccttcgt ccgctctccc cttctctttt ctgccttttt
    18061 tagccaggat taacgctcag ttaacttacc agagtattgc ggagccgttg tgaccaggac
    18121 caacctcatc accggttttc tcggcagcgg gaaaaccacg tcgattcttc atctgttagc
    18181 ccataaagat cccaacgaaa aatgggcggt actggttaat gaatttgggg aagtcggaat
    18241 tgatggtgct ttgctcgccg atagcggcgc attgctgaaa gagatccccg gcggctgcat
    18301 gtgctgcgtt aatggtttac ccatgcaggt agggttgaat accttactgc gtcagggaaa
    18361 accagaccgc ttgttgatag agccgaccgg gctgggccat ccgaaacaga tcctcgatct
    18421 gttaaccgca ccagtctatg aaccgtggat agatctgcgc gccaccttgt gcattctcga
    18481 tccacgcctg ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg accagctggc
    18541 tgccgcagac atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc
    18601 gctacagcgt tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg
    18661 gaaagttgac ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag
    18721 cgccgcgcat tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga
    18781 gcatcaacgc tggcgtcgca gtctgaacag cgggcaagga tatcaggcct gcggctggat
    18841 attcgacgct gatacggtat tcgacaccat tggcattctg gaatgggcgc gacttgcacc
    18901 ggtggaacgc gtcaaaggcg tgctgcgtat tcccgaaggg ctggtgcgaa tcaaccgtca
    18961 gggcgatgac ctgcacattg aaacgcaaaa cgttgcgcca ccggacagtc gtattgagct
    19021 gatttccagc agcgaagctg actggaatgc tttgcagagc acgctgttga agcttcgttt
    19081 agcgactacc gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga aaatatgatt
    19141 aaaaatttgc cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg
    19201 tatattcccg ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt
    19261 aatcagaaac tggtcgaaag taaggccttt ttgtggctgg ttgcattgac caacaatcgc
    19321 gccttcgacg gttgttcact gctggcgatg ggtatgttga tgctgagttt ctggctgaaa
    19381 gaaaacgccc caggcagacg acgtatcgtg attatgggtc tggtcatgct attaactgca
    19441 gtggtattaa accagctggg tcaggcatta attcctgtaa aacgggccag cccaacattg
    19501 acttttaccg acattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca
    19561 cgagatagct ttcccggcga tcacggcatg atgctgctta ttttttcggc attcatgtgg
    19621 cgttatttcg gcaaagttac aggtcttatc gcccttatta tttttgtggt ttttgcgttt
    19681 cccagagtaa tgattggcgc acactggttt actgacatca ttgtcggttc gatgaccgtg
    19741 atattgatcg gtttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt
    19801 tttgacaaat cactaccggg aaaaaacaaa catttccaaa acaaataact cacagtaatt
    19861 aacatcatca ggtttatttt tatagtgagg ataatcctga tgatgcgcac cgtgctttca
    19921 tctatcgaac gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa
    19981 tcgtacttta attgaccaat cccgcttatt aactttctgt atcacttttt cttataaaaa
    20041 atcatgtaaa accgctcgcc aagaccgcac caatcgggta atctcgaact cgttttgcct
    20101 cggcggtaga ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta
    20161 aggaattgtt tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt tttttataac
    20221 gatatttgtc gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt
    20281 ttgagatata tcttgcgcgg gattcccgcg attgcagtag cggttctgct ttctgcatgt
    20341 agtgcaaata acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca
    20401 tcatcactgc aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa
    20461 tcgcgaatta tggatcagta tgctgactgg aaaggcgtac gttatcgtct gggcggcagc
    20521 actaaaaaag gtatcgattg ttctggtttc gtacagcgta cattccgtga gcaatttggc
    20581 ttagaacttc cacgttcgac ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt
    20641 aatttgcgta cgggtgattt agttctgttc cgtgccggtt caacgggacg ccatgtcggt
    20701 atttatatcg gtaacaatca gtttgtccat gcttccacca gcagtggtgt tattatttcc
    20761 agcatgaatg aaccgtactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc
    20821 agctaataaa ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact
    20881 gcttaggcag tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat
    20941 ccaggctata gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac
    21001 ggaatcatgt tcatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgta
    21061 gcaggcgtga tgattgcgat actggttagc tgccttcagt ttttagtggc ctggcataag
    21121 cacgaagtca aatacgacac actgattatc gacgtacaaa agtatctcga tacctatttt
    21181 gccgacctga aatccactac tgaccggctt cagccgctga ccttagatac ctgccagcag
    21241 gctaaccccg aactgaccgc tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg
    21301 gtgaaagata aaaaaacatt ctgttcatct gcgactggtg agatggacat tccactcaat
    21361 gaattgattc cggcgctcga cattaataaa aacgtcgata tggcgatctt acccggcacg
    21421 ccgatggtgc cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat
    21481 agcggcgtct ttgccgctct gaatctcaac ctgacgcctt cactctttta tagttcacgg
    21541 caggaagatt acgatggcgt cgccctcatt attggcaata ctgcgctatc taccttttct
    21601 tcacgtttga tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac taaaattgcg
    21661 ggcattcctc tgaccgttcg gctttatgca gatgactgga catggaacga tgtgtggtac
    21721 gcatttttac tgggcggcat gagtggaact gtcgttggcc tgctctgcta ttacctgatg
    21781 agcgtacgta tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga acaattttac
    21841 gtggcgtatc aaccggtggt ggatacacaa gctttacgag tcacgggcct ggaagtactg
    21901 ctacgctggc ggcatcctgt cgcgggagaa attcccccgg atgccttcat taactttgcc
    21961 gaatcgcaaa agatgattgt gccgctgact cagcacctgt ttgagttaat tgcccgcgat
    22021 gccgcagaat tagaaaaagt actgccggta ggcgtcaaat ttggcattaa cattgcgccg
    22081 gaccatctgc acagcgaaag ctttaaagca gatatccaga aactgctcac ttccctgccc
    22141 gcacaccatt tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcaagaa
    22201 gccacacaac tcttcgcctg gctgcactcg gtcggcgtag aaattgctat tgatgacttc
    22261 ggcaccgggc acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt
    22321 gaccgtggat ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg
    22381 gtgctgacgc tggcgaaacg tctcaatatg ctgacagttg ctgaaggggt cgaaacgccg
    22441 gaacaagcgc gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt
    22501 cgcccgttac cgctggacga ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg
    22561 taaggtgtgc ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga
    22621 tgagtaaaag gaaatccgtt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg
    22681 ttcacctttg gtgtgcaggc gcaggctatc aaggaaagct atgcctttgc cgtgctgggc
    22741 gaaccccggt acgcgtttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa
    22801 ggtgggcaga taacgttgtc agccctcggc accttcgata atttcaaccg ctatgcactg
    22861 cgcggcaacc cgggcgcacg caccgagcag ctgtacgaca cgctatttac gacttccgat
    22921 gacgaaccag gcagttatta cccgctgatt gctgaaagcg cacgctatgc tgacgattat
    22981 tcctgggtgg aggtcgctat taatccgcgc gcccgttttc atgatggttc gcccattact
    23041 gcccgcgatg tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt
    23101 ctggtctaca aaggcaccac cgtcaaagcc attgcgccgt taaccgtgcg cattgagtta
    23161 gctaaacccg gcaaagaaga tatgttgagt ctgttttcgc tgccggtatt tccagaaaag
    23221 tactggaagg atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg
    23281 taccgcatta cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac
    23341 tgggcagcaa acttaccggt aaaccgtgga cgctggaatt tcgacaccat tcgctacgat
    23401 tattacctcg atgata
//
LOCUS       DAZFVO020000074        23397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFVO020000074 DAZFVO020000000
VERSION     DAZFVO020000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.074"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHKNB_RS13290"
                     /old_locus_tag="pgaptmp_002658"
     CDS             complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHKNB_RS13290"
                     /old_locus_tag="pgaptmp_002658"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HGU4196146.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAA"
     gene            complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHKNB_RS13295"
                     /old_locus_tag="pgaptmp_002659"
     CDS             complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHKNB_RS13295"
                     /old_locus_tag="pgaptmp_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HGU4196147.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1127..1954
                     /gene="nadE"
                     /locus_tag="ACHKNB_RS13300"
                     /old_locus_tag="pgaptmp_002660"
     CDS             1127..1954
                     /gene="nadE"
                     /locus_tag="ACHKNB_RS13300"
                     /old_locus_tag="pgaptmp_002660"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGU4196148.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2184..3071
                     /gene="cho"
                     /locus_tag="ACHKNB_RS13305"
                     /old_locus_tag="pgaptmp_002661"
     CDS             2184..3071
                     /gene="cho"
                     /locus_tag="ACHKNB_RS13305"
                     /old_locus_tag="pgaptmp_002661"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HGU4196149.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHKNB_RS13310"
                     /old_locus_tag="pgaptmp_002662"
     CDS             complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHKNB_RS13310"
                     /old_locus_tag="pgaptmp_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HGU4196150.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3809..4294)
                     /gene="spy"
                     /locus_tag="ACHKNB_RS13315"
                     /old_locus_tag="pgaptmp_002663"
     CDS             complement(3809..4294)
                     /gene="spy"
                     /locus_tag="ACHKNB_RS13315"
                     /old_locus_tag="pgaptmp_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HGU4196151.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4624..5592)
                     /gene="astE"
                     /locus_tag="ACHKNB_RS13320"
                     /old_locus_tag="pgaptmp_002664"
     CDS             complement(4624..5592)
                     /gene="astE"
                     /locus_tag="ACHKNB_RS13320"
                     /old_locus_tag="pgaptmp_002664"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HGU4196152.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDHRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5585..6928)
                     /gene="astB"
                     /locus_tag="ACHKNB_RS13325"
                     /old_locus_tag="pgaptmp_002665"
     CDS             complement(5585..6928)
                     /gene="astB"
                     /locus_tag="ACHKNB_RS13325"
                     /old_locus_tag="pgaptmp_002665"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HGU4196153.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6925..8403)
                     /gene="astD"
                     /locus_tag="ACHKNB_RS13330"
                     /old_locus_tag="pgaptmp_002666"
     CDS             complement(6925..8403)
                     /gene="astD"
                     /locus_tag="ACHKNB_RS13330"
                     /old_locus_tag="pgaptmp_002666"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HGU4196154.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8400..9434)
                     /gene="astA"
                     /locus_tag="ACHKNB_RS13335"
                     /old_locus_tag="pgaptmp_002667"
     CDS             complement(8400..9434)
                     /gene="astA"
                     /locus_tag="ACHKNB_RS13335"
                     /old_locus_tag="pgaptmp_002667"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HGU4196155.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9431..10651)
                     /gene="astC"
                     /locus_tag="ACHKNB_RS13340"
                     /old_locus_tag="pgaptmp_002668"
     CDS             complement(9431..10651)
                     /gene="astC"
                     /locus_tag="ACHKNB_RS13340"
                     /old_locus_tag="pgaptmp_002668"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HGU4196156.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11097..11903
                     /gene="xthA"
                     /locus_tag="ACHKNB_RS13345"
                     /old_locus_tag="pgaptmp_002669"
     CDS             11097..11903
                     /gene="xthA"
                     /locus_tag="ACHKNB_RS13345"
                     /old_locus_tag="pgaptmp_002669"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGU4196157.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12070..12780
                     /locus_tag="ACHKNB_RS13350"
                     /old_locus_tag="pgaptmp_002670"
     CDS             12070..12780
                     /locus_tag="ACHKNB_RS13350"
                     /old_locus_tag="pgaptmp_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4196158.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12785..13462
                     /gene="ydjY"
                     /locus_tag="ACHKNB_RS13355"
                     /old_locus_tag="pgaptmp_002671"
     CDS             12785..13462
                     /gene="ydjY"
                     /locus_tag="ACHKNB_RS13355"
                     /old_locus_tag="pgaptmp_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HGU4196159.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13477..14184
                     /locus_tag="ACHKNB_RS13360"
                     /old_locus_tag="pgaptmp_002672"
     CDS             13477..14184
                     /locus_tag="ACHKNB_RS13360"
                     /old_locus_tag="pgaptmp_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGU4196160.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14184..14732
                     /locus_tag="ACHKNB_RS13365"
                     /old_locus_tag="pgaptmp_002673"
     CDS             14184..14732
                     /locus_tag="ACHKNB_RS13365"
                     /old_locus_tag="pgaptmp_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGU4196161.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14742..15908
                     /locus_tag="ACHKNB_RS13370"
                     /old_locus_tag="pgaptmp_002674"
     CDS             14742..15908
                     /locus_tag="ACHKNB_RS13370"
                     /old_locus_tag="pgaptmp_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4196162.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15881..17416
                     /locus_tag="ACHKNB_RS13375"
                     /old_locus_tag="pgaptmp_002675"
     CDS             15881..17416
                     /locus_tag="ACHKNB_RS13375"
                     /old_locus_tag="pgaptmp_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGU4196163.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17416..18069
                     /locus_tag="ACHKNB_RS13380"
                     /old_locus_tag="pgaptmp_002676"
     CDS             17416..18069
                     /locus_tag="ACHKNB_RS13380"
                     /old_locus_tag="pgaptmp_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4196164.1"
                     /translation="MLCVKNVSLHLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18136..19443
                     /gene="ynjE"
                     /locus_tag="ACHKNB_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /pseudo
     CDS             18136..19443
                     /gene="ynjE"
                     /locus_tag="ACHKNB_RS13385"
                     /old_locus_tag="pgaptmp_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(19452..20072)
                     /locus_tag="ACHKNB_RS13390"
                     /old_locus_tag="pgaptmp_002678"
     CDS             complement(19452..20072)
                     /locus_tag="ACHKNB_RS13390"
                     /old_locus_tag="pgaptmp_002678"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HGU4196165.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20159..20566
                     /gene="nudG"
                     /locus_tag="ACHKNB_RS13395"
                     /old_locus_tag="pgaptmp_002679"
     CDS             20159..20566
                     /gene="nudG"
                     /locus_tag="ACHKNB_RS13395"
                     /old_locus_tag="pgaptmp_002679"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HGU4196166.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20532..20804)
                     /locus_tag="ACHKNB_RS13400"
                     /old_locus_tag="pgaptmp_002680"
     CDS             complement(20532..20804)
                     /locus_tag="ACHKNB_RS13400"
                     /old_locus_tag="pgaptmp_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HGU4196167.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21040..22383
                     /gene="gdhA"
                     /locus_tag="ACHKNB_RS13405"
                     /old_locus_tag="pgaptmp_002681"
     CDS             21040..22383
                     /gene="gdhA"
                     /locus_tag="ACHKNB_RS13405"
                     /old_locus_tag="pgaptmp_002681"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGU4196168.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22500..>23397)
                     /locus_tag="ACHKNB_RS13410"
                     /old_locus_tag="pgaptmp_002682"
     CDS             complement(22500..>23397)
                     /locus_tag="ACHKNB_RS13410"
                     /old_locus_tag="pgaptmp_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4196169.1"
                     /translation="VVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFK
                     AKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQY
                     HVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQV
                     IALLNKFAVFSSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAW
                     HYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEH
                     YSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5592 a         6197 c         5966 g         5642 t
ORIGIN      
        1 cgcagcctta agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc
       61 aaccggtttg ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc
      121 taataacacg acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc
      181 aataatgacc ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga
      241 ggtagacatg cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc
      301 atcgacgatt atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg
      361 taacttgcga aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg
      421 agcaatagta tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc
      481 ttcacatttc gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg
      541 cgcaatggcc ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct
      601 gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat
      661 catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct
      721 ggcaagtacc gcgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg
      781 caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt
      841 ctttagtacg atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca
      901 gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag
      961 cctggaatac attgggcgct ttttcaagcc cgtgaacgaa acagctccgc tttcagagga
     1021 ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtagcaac
     1081 gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca
     1141 acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg
     1201 tagtgtcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct
     1261 cgggatcagc ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa
     1321 tgagctgcgc caggaaactg gcaacgaatc actgcaattt attgccgtac gcctgcccta
     1381 tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg
     1441 cgtattaacc gttaatatca agggcgcggt attggctagc gagcaggcat tgcgggaagc
     1501 aggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc
     1561 acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga
     1621 agccattacc ggattcttca ctaaatatgg tgacggcggt acggacatta atccgctgta
     1681 tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct
     1741 ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt
     1801 ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga
     1861 acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc
     1921 aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacctattg gattgtgcga
     1981 aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta
     2041 tgcaaattca atacattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt
     2101 gtaacctttg tcatcaaccc gcctcttttt taatcgcctc ccgcctgtta cactggatag
     2161 ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg
     2221 aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca
     2281 cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca
     2341 aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca
     2401 tgctacggca atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc
     2461 tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca
     2521 atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag
     2581 aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt
     2641 tgcaagcatt gcagagcatc gctgatgaac aaaaactttg ttatggcctg ttgggactgg
     2701 aaccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat
     2761 gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt
     2821 tgcgggtggt gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa
     2881 tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag
     2941 aagcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt
     3001 gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc
     3061 gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg
     3121 cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca
     3181 cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc
     3241 ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga
     3301 catctgccct gccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg
     3361 taacggtttt aaggtatggt taaagcggtc tgcgctttca aggaacatct cgccgccttc
     3421 cagcaacgtc actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat
     3481 ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc
     3541 gcgcgtttca ccggcagcgt ttcgccacag attcaccgac attttacgca tatcaaagta
     3601 ttccatactc gctcccggcc cgttcttttt attgttatcg ttcaggtaac cgtggatggt
     3661 tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag
     3721 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc
     3781 ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg
     3841 ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg
     3901 gcgtcaggat gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc
     3961 gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg
     4021 caatgatgtc atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct
     4081 ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga
     4141 acatcatgtc ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag
     4201 ccggtgctgc ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg
     4261 tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt
     4321 tattacggct ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg
     4381 tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta
     4441 tggagaaaaa aagaaaattt gatggagagt gatgagagaa taatacaaca cgatgatttt
     4501 gcagagatta tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat
     4561 taatctgtat taatttataa gaaagcaact taatacccgc agaatgattt ctgcgggtaa
     4621 gtattagctt attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt
     4681 agggaataac acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc
     4741 cagcaatgtt cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc
     4801 gaaggacggc gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct
     4861 cacgataccg acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa
     4921 ctggcgatga tcgttttgcc caaagggcaa cgctttgcca agttccagcg tacaggccag
     4981 cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca ccaggttcct gatggaacac
     5041 cagcgcctcc agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc
     5101 gcgttgcggt aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc
     5161 aaggtgccag cgcacagatt ctttgcccag gtcataaaaa tcttccaggc actgttccag
     5221 ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat
     5281 tcgattcata tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag
     5341 gatcaccagc aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc
     5401 gtccagcatc tccacaggtg ccgtctcatt accgtgtatt cccgctgaaa tcaccagtgc
     5461 gccttgcggt ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg
     5521 aacgccgttg atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag
     5581 aaaattatcc attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta
     5641 attggctcaa tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat
     5701 cggcagcagt aaggcgatcg cggtagtaac gatccaccca gtcattgagc gcattaaaca
     5761 gcgtatcgtt catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaatacca
     5821 cccgcaaccg caggcacgcc gggccgccgc cattcgccat gctttcacgt aaatcaaaga
     5881 cttttagttc gctaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc
     5941 cggcgtgttc ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca
     6001 acagttggct gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg
     6061 gaacttctat cgccataaaa ccgttcaccc gcgcacgcag gtttgccagt aactgtgact
     6121 ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg gttactcacg gcaatcacgt
     6181 cattatgaaa aacgccctgg tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt
     6241 ggggattcac ctgattcagc cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg
     6301 gataacggga aggccgggta tcattgcctt cttctcgccc gtagacaaaa agttgcatac
     6361 ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc cgccccctca tcaccgagca
     6421 acgctacctg cggcaacgcc gaatgaacgc taaatttctc ttcgtcgtta aaaatcgctt
     6481 ttaaaagtga ttcggtgacg tgcgcttcca gcgagcggtg aaatttattg ttcaggttgg
     6541 caacggtgag atgcactttg ccatccagcg tatcggcaga tggcgcgatc gttgccgcat
     6601 tggctaccca cattggcgaa gcggaactga cgctggaaag ccagtgcggt gcctggcgtg
     6661 caactttttc cagtacctgc tcatcgctac cactgaatcc caactgacgc agcaccggaa
     6721 taaacggacg ctcgtgcggc gggatcacgg cctgggggaa tcccgcatcg gcaagggctt
     6781 tcattttcag taagccctgt tttgccgcct gtcgcgggtt agacacctga aaacggtgac
     6841 gggtagaggc ttcattacca aacgacaggc cagcgtaatg atgcgtcagc cctaccagcc
     6901 cgtcgaaatt aacttcccag gcgttcatcg caccacctca tcggaaaaat ccagcccggg
     6961 gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg ctcgccatag gccatgcgca
     7021 gtaatctgcg gcataccagg cgctggggcg atggttaccg gatgcaccaa tgccgccgaa
     7081 tggcgcggta ctggcagcac cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc
     7141 ctccagcaac agttgatcga atttttcccg ctcgggggaa accagaccgc aagagagtcc
     7201 gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa gaatcataac gccagacgcg
     7261 caataacggt ccgaacacct cttcatctgg tacgccagca acgcctgtca tttcaatgat
     7321 ccccggcgtc agcaacgatg tctctgattg taataagcgc ggcgcaagca gggttcgtcc
     7381 gcccatcgct tccagttgct gccaggcagt aaccacctgc tgtgcggcct gttcagaaat
     7441 cagcccgcca ataaacggct gcggttcgtc atcccagttg cccggcgtta atcgctggct
     7501 gacggcaacc agacgagcaa gaaacgcatc gccctgcgct ccgcttttga gcaataaacg
     7561 gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc gactgaatgg tcagatggac
     7621 agccgcgtcg atatccgcca cctcatcgat aattagcgga ttattaccgc ccatctcaag
     7681 ggcgagaatt ttctccggct gaccggagag ctggcgatgc agctggtagc cggtattggc
     7741 gctaccggta aacagcaaac cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc
     7801 acgcccgccc tgcaccaggt tcagtacgcc cggcggcaag ccagcctgct gccataaacg
     7861 cattaccgct tcgccactcc acggtgtcag ttcgctgggt ttaaagataa tggtgttacc
     7921 tgccagcaat gccggaacga tatgtccgtt cggcaaatga ccagggaaat tatacggccc
     7981 aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc
     8041 actacgctgc tcgccggtac gagcgtgata cgccttaatt gatatcgcga ttttattgat
     8101 catcgccgtc acttcggttg ccgcttccca gcgcggcttg ccggtttctc gggcaatgat
     8161 tgcggttaat tcgcctttat tcctttccag caatccggca aagcgttcga caacgacctg
     8221 acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc
     8281 ctgctcgacc tgagcggcat cggcatcatt gccttgccat aacacctcgc ccgataccgg
     8341 attacgcttc acacgcgatg cgccctggcc cgttatccag tcaccgttaa tccataaagt
     8401 catgctgttt tctcctctgc gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat
     8461 ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct cggttgccgg atcggtacgc
     8521 gccagcacca cgcggaaatg gtgataattt tcattggcga ccaggcaggc cggaaaatcg
     8581 ccctgcgcag gctgcccttc tgccacttcc accagccgac ttttacgaat ggcgcgcacg
     8641 cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa
     8701 cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg
     8761 atgacgtcct gggcttcctg ggataaaaag tgggtataga tcggatgttt cggcatcagt
     8821 tctgcaataa atgccttttg cccggtgccg cagagaaaat cggcgcggct aaaatccatc
     8881 gaaaagaagc gtttaccgag gctttgccag aacggtgaat agccgtgttc gtcaatcacc
     8941 ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc gaaaagccgc cataaacata
     9001 aagcgcgatt tcgacagcaa atagccgttg ccctctttgc gccagtccgg gtcgagaaac
     9061 agcgtgcaca gctcgctgct gccggtgtga tcgttactga gaaacagcgt cggcaatgcg
     9121 ttatagacat tcagctcttt tgaggcgtga accaacgtgc cgacgcgata gttgtaccag
     9181 ggatcgttca gcccaaccgc cacctcaatg gcacaaatcc ccgccacggt gcctgtctcg
     9241 ctatcttcca gcacgaacac atagccctgc tcacttttgg gcagttcgcc ttgccaggtt
     9301 ttgattgccc tttcgatacg cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg
     9361 ccgccgcccg ttttgctggc aagctgcatc agcgccgaga catctgatcg ctcaacggga
     9421 cggatgacca tcatgatgaa cctcggctaa caaagtgttc gcaagcagct gcaaagcgat
     9481 ccagtccggt cgtcacctct tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt
     9541 tgccacccgc aatcagtacc atcacgcctg ctttcgccgc ttcctgagag atctgtttcg
     9601 cttgcccggc gtaatcggca ttcagtacac agccaatcag caaacctaag ccgcgaactt
     9661 cactgaacaa accatagcgg tgattaatag tattaagacg ctcaacaaac cagtcgtgac
     9721 gctgtttaac gccattaagc atctctggtg tgttgatgag ctccagcact ttgcctgcca
     9781 ccgccgaggc cagcggatta ccgccatagg tggtgccatg agtgccaacg gtcatcacgc
     9841 gggcgcactc ttcggttgcc aacaacgcac cgaccgggaa accgccgccc agcgctttgg
     9901 cggtagttaa cagatcaggc gtcacgccgt agtgcatata ggcatacagt tccccggtgc
     9961 gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc attgtggcgg ttacacaatt
    10021 cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac accgccttcc ccctggatgg
    10081 gttcgacaat caccgcacag gtagagtcgt caatcagcgc gctggcagag ttaatatcgt
    10141 tatatgcagc atgacgaata tccgccggca gtggcgcaaa atcctgtgaa taggctggct
    10201 gcccacccgc actgacagta aacagcgtgc gaccatgaaa cgcatttttg aacgccacga
    10261 tgccgctctt atggctgccg tagcggtcgt gagcgaattt acgcgccagt tttagcgccg
    10321 cttcgttggc ttccgcaccg gagttacaaa agaagacgcg atcggcaaac gtggcgtcga
    10381 tcaatttttt cgccagtcgc agtaccggct cgttggtgta accgttgccg gtatgccaga
    10441 acttactcgc ctgttcgttc agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt
    10501 tcaccgcaat gccacccgcg aagtcgatat actctttccc ctgctgatcc cacaagcgcg
    10561 aaccttcgcc acgtaccggt ataaagggtg ccggagcgta aacaggtatc atccattcat
    10621 caaagttttc acgcgtaatt ggctgagaca tagcgacctc tacagtaaat aattcgttat
    10681 ttatatgtta acaataagta atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag
    10741 ccagtgataa aagtgcataa acggcggagg ctaactggaa atcaaggagt tataaccaaa
    10801 ccatatgcat ttaaagtgca tataaagtga atacgtttgc gatgtgggtg aataaaaaga
    10861 ataaaaaacg caatgttatg cagaagtaaa atataattct ggaattgtga tcattgacga
    10921 aatttactgg aaattactgc gccattctga cgcagcgcgc accaaaagcg ggcatttttt
    10981 gcgccatcgt tgacatcatt aacaaccatc gatcaaatca cttaacaaca ggcggtaagc
    11041 aacgcgaaat tctgctacca tccacgcact ctttatctga ataaatggca gcgactatga
    11101 aatttgtctc ttttaatatc aacggcctgc gcgccagacc tcaccagctt gaagccatcg
    11161 tcgaaaagca ccaaccggat gtgattggcc tgcaggagac aaaagttcat gacgatatgt
    11221 ttccgctcga agaggtggcg aagctcggct acaacgtgtt ttatcacggg cagaaaggcc
    11281 attatggcgt ggcgctgctg accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg
    11341 gtgacgacga agaggcgcag cggcggatta ttatggcgga aatcccctca ctgctgggta
    11401 atgtcaccgt gatcaacggt tacttcccgc agggtgaaag ccgcgaccat ccgataaaat
    11461 tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta cctggaaacc gaactcaaac
    11521 gtgataatcc ggtactgatt atgggcgata tgaatatcag ccctacagat ctggatatcg
    11581 gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg
    11641 aagagcgcga atggatggac aggctgatga gctgggggtt ggtcgatacc ttccgccatg
    11701 ctaatccgca aacagcagat cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg
    11761 ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca accgctggca gaatgttgcg
    11821 tagaaaccgg catcgactat gaaattcgca gcatggaaaa accgtccgat cacgcccccg
    11881 tctgggcgac cttccgccgc taatttagca gctctcctgg ctcaaactgg gtcaggagaa
    11941 ttaaccttga gaaaaatcaa caaactgtca gtaatgattt gttgcctgcc gtcctttgtt
    12001 ataccgtcac tgcgttttta gttgtctgac cacttctcta ttatcaagtt tgatatagga
    12061 aactccacga tgaacgctga gcgtaacttt ctgtttgcct gtcttatttt tgcgctggtc
    12121 atttacgcta tccacgcttt cggtttattc gatctgctca ccgatttacc ccacttacag
    12181 acactcattc gccagagcgg attgttcggt tatagcctct atattctgtt attcatcatt
    12241 gccaccctct ttctgttacc aggaagcata ctggtgatcg ctggaggaat agtttttggc
    12301 ccgctcttag ggacactact ctcattaatt gccgcaacgc tggcctcctc gtgctcattc
    12361 ctgctggcgc gctggctggg acgtgattta ctgctgaaat acgtgggcca tagccatacc
    12421 ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg attttcttat tctgacccgc
    12481 ttaatcccgt tgtttcctta caatattcaa aattacgctt acggattaac caccatcgcc
    12541 ttctggcctt atacccttat ttcggcactc acgaccctgc ccggtattgt tatttatacc
    12601 gtgatggcaa gcgatctcgc caatgaaggc attacgctgc gctttatttt acaactctgt
    12661 ctggcgggcc tggcgctgtt tattctcgtc cagctcgcaa aactctacgc ccgacacaaa
    12721 catgtggatc tgtctgcttc gcgccgcagc ccacttactc acccaaaaaa tgaaggatag
    12781 aacgatgtcg caacattatt cagtgtcatg gaaaaaagga ctggctgcac tctgcttact
    12841 ggctgttgcc gggcttagcg gctgcgatca aaaagagaat gcggcggcaa aagtggaata
    12901 cgacggactt tcgaacagcc aaccgctgcg cgtcgatgcc aataaccata cggtaaccat
    12961 gctggtgcaa attaatggtc gtttcctcac cgacgacact cgtcacggta ttgtgtttaa
    13021 agatggctcc aacggacata aatcgctgtt tatggcttat gcgaccccga aagcatttta
    13081 tgaagccctg aaagaggcag gtggtacgcc gggcgaaaac atgacgatgg ataataaaga
    13141 aacgactcat gtcacaggca gcaaactgga tatttcggtc aactggcaag gggcggcaaa
    13201 agcgtattcc ttcgatgaag tgattgttga cagtaatggc aagaaactgg acatgcgctt
    13261 tggcggtaat ttaacggcag cagaagagaa gaaaacgggt tgcctggtgt gtctggatag
    13321 ctgcccggtc ggcatcgtca gcaatgcaac atacacttat ggtgcggtgg aaaaacgcgg
    13381 cgaagtgaaa ttcaaaggca atgcctcagt tctcccggcg gataacacgc tggcaacggt
    13441 tacctttaaa atcaccgaat aaagccagga taaaggatga tgaagataca atcgcgtaaa
    13501 atctggtact accgtataac cctcatcatc ctgttgttcg ctatgctgct cgcatgggcg
    13561 ctgcttcccg gcgtccatga gtttatcaat cgcagcgttg cggcgtttgc cgccgtggac
    13621 caacagggta tagaacgctt tattcagtct tacggcgcac tggcggcagt tgtctcgttc
    13681 ttgttgatga ttttacaggc cattgctgca ccgctacctg cgtttttgat cacctttgcc
    13741 aatgcgtcgc tgtttggcgc gttctggggc ggcttgctgt cgtggaccag ttcgatggcc
    13801 ggcgcggcgc tgtgcttttt tatcgccaga gtgatgggcc gcgaagtggt ggaaaaatta
    13861 accggcaaaa ccgtgcttga cagtatggac ggctttttca ctcgctacgg caaacacacc
    13921 atcctggtct gtcggttatt gccttttgtc cctttcgatc caatcagcta tgctgccggt
    13981 ctgacttcaa tacgttttcg ctcgtttttt atcgccaccg ggcttggtca attaccggcg
    14041 actattgttt attcctgggc gggcagcatg ttaacaggcg gtactttctg gtttgtcacc
    14101 ggactgttta ttctgtttgc cctgaccgtg gtgattttta tggcgaagaa aatatggctt
    14161 gagcgccaga agaggaatgc ctgatgggtt taccgccgct tagcaaaatt cctttcattt
    14221 tacgtccgca ggcgtggctg catcgtcgcc attacggcga ggtgctaagc ccgattcgct
    14281 ggtgggggcg gatcccgttt atcttttatt tggtgtcaat gtttgttggg tggctggagc
    14341 gcaaacgctc accgctcgat ccggtagtgc gatcgcttgt cagcgcgcgc attgcgcaaa
    14401 tgtgcctgtg tgagttttgc gtggatatca ccagtatgaa agtcgccgag cgtaccggca
    14461 gcaccgataa actgctggca gtggctgact ggcggcaaag cccgctcttc agcgatgaag
    14521 aacggctggc gctggagtac gccgaagccg caagcgtaac gccgccaacg gtcgatgatg
    14581 ccctgcgcac ccgactggct gcgcattttg acgctcaggc gctcaccgaa ctgacggcat
    14641 tgatcggcct gcaaaacctg tcggctcgtt ttaattctgc catggacatt cccgctcagg
    14701 ggctgtgccg tattccggaa aaacgttctt aaggagagaa gatgcgccat tgtgggtggt
    14761 tgctgggatt gttatcgctg ttttctctgg caacacatgc cagtgactgg caggaaatta
    14821 aaaatgaggc caaagggcaa accgtctggt ttaacgcctg gggcggcgat accgcaatta
    14881 accgctatct cgactgggtt agcggcgaga tgaaaaccca ttacgctata aacctgaaga
    14941 ttgttcgtct ggcggatgcc gcagacgcgg tgaagcgcat tcagaccgaa gctgctgccg
    15001 gacgtaaaac gggcggctcg gtggatctgc tctgggtgaa cggcgaaaac ttccgcacct
    15061 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
    15121 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc tacagaaggg gctgaatcgc
    15181 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
    15241 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acctatccgc
    15301 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgcccg
    15361 atcccgccgc gttaaaagtt gccccggata aagccacttt cgcccgcgtc actgcgccct
    15421 tgtggcaata tcttgacgcg ctacatccct atttatggcg caaaggaaag gatttcccgc
    15481 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
    15541 ttaaccctgc tcatgcccag caaaaaatcg ccagcggcga tttgcctgca agcagttaca
    15601 gttttggttt taaccagggg atgattggca acgtgcattt cgtcaccatt cctgctaacg
    15661 caaatgccag tgctgcggcg aaggtggttg ccaacttcct gctctcaccc aatgcgcaac
    15721 tgcgtaaagc ggatcccgct gtctggggcg atccttctgt tctcgatccg caaaaactgc
    15781 ctgatgggca gcgcgaaaca ttgcaatcaa gaatgccgca agatctgccg ccggtactgg
    15841 ctgaaccgca cgcaggttgg gtaaatgcgc tggaacaaga atggctacac cgttacggta
    15901 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccac tgatcccggc
    15961 agctctcacg ctgatatcgc ctgctttgtc gttgacacac tggcaggcgt tatttgccga
    16021 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
    16081 ggcgttgttg attgccctgc tggtgattgt ggcgctgtgg ccttggccga aatggcagcg
    16141 tatgtgtgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
    16201 ccttctgctc tttgctgagg gagggctgct ttatgactat ttcccgtatt tcactccgct
    16261 aatggacaaa ttgggtattg ggctgggcct cacccttgca gtgaaagaaa gcgcatttct
    16321 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
    16381 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctcttgc cctccgtcgc
    16441 acctgcactg gcaatggcga tgttggcgat tgttgcctgg tcgctttcgg tcgtggatgt
    16501 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
    16561 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
    16621 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
    16681 tccccgcgta gatggcattc gcaagcctgc cacaccttta ttaccaggca ctacgctggc
    16741 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    16801 gtcgacgatc aatagtgaag cactcatcaa cagcctgaca atggggctgg tggcgacatt
    16861 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    16921 gctctggttg cccattctat tgcccgctct gccactggtg gcgggccagt acacgctggc
    16981 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    17041 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    17101 tttgattgcg caaacactga gctggtcgcg ggccaaaatc ttcttttacg tgaaatgtcc
    17161 gcttatgcta cgccctacgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    17221 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    17281 ggcattaagc agcggcggca gcaatggtat tttcgccgcc caggctttat ggcaactgct
    17341 attaccactt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    17401 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacatttacc agaaagccgc
    17461 ttgctgacaa acgttaactt tacggtggat aaaggtgaca ttgtcacgtt aatggggccg
    17521 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    17581 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    17641 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    17701 aatttactgc tggcgctacc gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    17761 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    17821 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    17881 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    17941 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    18001 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    18061 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    18121 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    18181 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacacc tgaccagctt
    18241 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    18301 caaaccttaa atggcccttc tggtcatgaa cctgccgcct aaaacctctc tgccagctgg
    18361 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    18421 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    18481 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    18541 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    18601 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    18661 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    18721 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    18781 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    18841 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    18901 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    18961 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    19021 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    19081 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    19141 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    19201 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    19261 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    19321 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    19381 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    19441 taacattgca attactgacg ctggtgcgac ttcagcgtca gatacccact ccacacccgc
    19501 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    19561 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taacccgccc
    19621 agataataaa acgatttatg cgcataaccg ggattatcaa tctgatgttt cgccgccagc
    19681 gcagcaaaag cgagaaaact gctgcctgtg ccgataaacg caaacagcag ccagccgcct
    19741 gccaaggcgt tttgctgcgg tgcggcgagg ataaacccaa aaggtaccag cgcgtaaaaa
    19801 agaaaatcga gagaaatatc aagaaagccg cccgcgtcgg taagccctct gcgccgcgcc
    19861 agcgcaccgt ccagaccatc aagcagcctg ttcaacaaaa tgacgacaag cgccgccaga
    19921 taccagccca gcgccagaaa aggcagtgcc agcacgccga tggcaaatcc cactaatgtt
    19981 aaaccatctg gcgtaatgcc cggtttatca agaacccgca cgcactgatg caacaacggt
    20041 ttaatccggg gatgaagatg gcggtctagc acaggcactc cttaaatata aagcctttct
    20101 gattgagcaa cagtgcggat attatggcat ttttcgctta tctgcccgtg tgtaatttat
    20161 gaaaatgatt gaagttgttg ccgccatcat tgaacgtgat ggcaaaattt tactcgcgca
    20221 acgccccgcc cagagcgatc aggcgggatt atgggagttt gccggtggta aagtcgagcc
    20281 ggatgaaagc cagcggcagg cgctggtgcg tgagttacgc gaagaactgg gcatcgaagc
    20341 aactgtgggt gagtatgtta ccagccatca gcgagaagtt tcgggccgga ttatccatct
    20401 tcatgcctgg cacgtacccg acttccacgg gacgttacag gcacatgaac atcaggcgct
    20461 ggtctggtgc tcacctgaag aggcgctgca atatccgctg gcccctgctg acattccatt
    20521 attagaggcg tttatggctt tacgcgccgc cagaccagcg gattagtgct aagggttttg
    20581 tcatcccgct ggcattgcag cagtattccc tcggctttaa ttaccgcgcc ttcagaataa
    20641 tttttatcct gataaacgca gcactgagta cagggctgcg ctgactgccc gcctgaactg
    20701 aacacttctg gcggtacgtt tacctccacg tccggacgat aatgcgggtt agccagtgca
    20761 attaatggaa atgctaatac tacggcgaac aatgctcgac tcacagggaa ctccttaacg
    20821 ttattgtctc tgctactgat aacggtagcc gggtggcaaa actttagcgt ctgagttatc
    20881 gcatttggtt atgagattac tctcgttatt aatttgcttt cctgggtcat ttttttcttg
    20941 cttaccgtca cattcttgat ggtatagtcg aaaactgcaa aagcacatca cataaacaac
    21001 ataagcacaa tcgtattaat atataagggt tttatatcta tggatcagac atattctctg
    21061 gagtcattcc tcaaccatgt ccaaaagcgc gacccgaatc aaaccgagtt cgcgcaagcc
    21121 gttcgtgaag taatgaccac actctggcct tttcttgaac aaaatccaaa atatcgccag
    21181 atgtcattac tggagcgtct ggttgaaccg gagcgcgtga tccagtttcg cgtggtatgg
    21241 gttgatgatc gcaaccaggt acaggtcaac cgtgcatggc gtgtgcagtt cagctcagcc
    21301 atcggcccgt acaaaggcgg tatgcgcttc catccgtcag ttaacctctc cattctcaaa
    21361 ttcctcggct tcgaacaaac cttcaaaaat gccctgacta ccctgccgat gggcggtggt
    21421 aaaggcggta gcgattttga cccgaaaggt aaaagcgaag gcgaagtgat gcgtttctgc
    21481 caggcgctga tgaccgagct ttatcgtcat ctgggcgcgg ataccgacgt tccggcaggt
    21541 gatatcggcg ttggtggtcg tgaagtcggc tttatggcgg ggatgatgaa aaagctctcc
    21601 aacaataccg cctgcgtctt caccggtaag ggcctttcat ttggcggtag tcttattcgc
    21661 ccggaagcta ccggctacgg tctggtttat ttcacagaag caatgttaaa acgccacggt
    21721 atgggttttg aagggatgcg cgtttccgtt tctggctccg gcaacgtcgc ccagtacgct
    21781 atcgaaaaag cgatggaatt tggtgctcgt gtgatcactg cgtcagactc cagcggcact
    21841 gtagttgatg aaagcggatt cacgaaagag aaactggcac gtcttatcga aatcaaagcc
    21901 agccgcgatg gtcgagtggc agattacgcc aaagaatttg gtctggtcta tctcgaaggc
    21961 caacagccgt ggtctgtacc ggttgatatc gccctgcctt gcgccaccca gaacgaactg
    22021 gatgttgacg ccgcgcatca gcttatcgcc aatggcgtta aagccgtcgc cgaaggggca
    22081 aatatgccga ccaccatcga agcgactgaa ctgttccagc aggcaggcgt actgtttgca
    22141 ccgggtaaag cggctaatgc tggcggtgta gcaacgtcag gccttgaaat ggcgcaaaac
    22201 gctgcgcgcc tgggctggaa agccgagaaa gttgacgcac gtttgcatca catcatgctg
    22261 gatatccacc atgcctgtgt tgagcatggt ggtgaaggtg agcaaaccaa ctacgtgcag
    22321 ggcgcgaaca ttgccggttt tgtgaaggtt gccgatgcga tgctggcgca gggtgtgatt
    22381 taagttgtaa atgcctgatg gcgctacgct tatcaggcct acaaatgggc acaattcatt
    22441 gcagttacgc tctaatgtag gccgggcaag cgcagcgccc ccggcaaaat ttcaggcgtt
    22501 tatgagtatt taacggatga tgctccccag ggaacatttc ttatgggcca acggcatttc
    22561 ttactgtagt gctcccaaaa ctgcttgtcg taacgataac acgcttcaag ttcagcatcc
    22621 gttaactttc tgcgatagca gcagatatgc cagtaaagaa atcccattag actatttttt
    22681 tgataatctt cttcgctttc gaacaactcg tgcgcctttc gagaagcaag cattatataa
    22741 tgccaggcca gttcttcttc aattgtcccg ttttgaaaag ctgtgcttga tatcgagatc
    22801 atccatgata attccgccgc ccatattagc ttcgccgagg atttaccgga gcgattatta
    22861 gcgcaatcag agatatagtc tgaggaaaaa acagcaaatt tattcaacaa ggcgataacc
    22921 tgctctgggg cttcctccat gtttgcttta aaggtattgg ctccatggtc gccagaaaga
    22981 aaatgctcca ttaaggcaca ataactttcg ctatcttcga taccccattg atcctctaaa
    23041 gactcgcgtc ttttacttat gatatcgatc gagtcaaaag gaagcacatg atattggaag
    23101 gtatctttgc caggttcagg ctttcgcggc cagaactcca gcgtttcaga ccattgctta
    23161 tgatagaatc gataaggtgc gatcaattgt agcgcctgta acttctcgat actgagcggc
    23221 tcaatacctt tagcctgata ataatgcagt tgttcttttt ttgctttaaa accggcccga
    23281 acaataagcc ccatcataat taatagataa agaaaagagc atcccgcagt aatcaggcct
    23341 ctttcattca aaccgttgga tgttatcgct gcgaatacaa acattacagc gacaaca
//
LOCUS       DAZFVO020000075        23333 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFVO020000075 DAZFVO020000000
VERSION     DAZFVO020000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.075"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..523
                     /locus_tag="ACHKNB_RS13415"
                     /old_locus_tag="pgaptmp_002683"
     CDS             <1..523
                     /locus_tag="ACHKNB_RS13415"
                     /old_locus_tag="pgaptmp_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4196170.1"
                     /translation="LSFPGGRKESIAERLKRLIGSRSVRAAARDWGLSFSTLNNYLSR
                     GTEPSLSVALQIASIEGVSVEWLCGFSDASQVNRCEIKENQDESKKIIMTIISALDEK
                     DLEQLSKNLVLNGAKYLARLLEPENQDLIRLEGRKRIAALQLEGMSDERVREILEETE
                     RSRKSNAVEPKAG"
     gene            complement(692..1405)
                     /locus_tag="ACHKNB_RS13420"
                     /old_locus_tag="pgaptmp_002684"
     CDS             complement(692..1405)
                     /locus_tag="ACHKNB_RS13420"
                     /old_locus_tag="pgaptmp_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HGU4196171.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAYNQVNH
                     LIPTRGLVKGGEITCKILK"
     gene            complement(1407..2195)
                     /gene="paaG"
                     /locus_tag="ACHKNB_RS13425"
                     /old_locus_tag="pgaptmp_002685"
     CDS             complement(1407..2195)
                     /gene="paaG"
                     /locus_tag="ACHKNB_RS13425"
                     /old_locus_tag="pgaptmp_002685"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HGU4196172.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(2195..2962)
                     /gene="paaF"
                     /locus_tag="ACHKNB_RS13430"
                     /old_locus_tag="pgaptmp_002686"
     CDS             complement(2195..2962)
                     /gene="paaF"
                     /locus_tag="ACHKNB_RS13430"
                     /old_locus_tag="pgaptmp_002686"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HGU4196173.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSAGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(2959..4029)
                     /gene="paaE"
                     /locus_tag="ACHKNB_RS13435"
                     /old_locus_tag="pgaptmp_002687"
     CDS             complement(2959..4029)
                     /gene="paaE"
                     /locus_tag="ACHKNB_RS13435"
                     /old_locus_tag="pgaptmp_002687"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HGU4196174.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGT
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(4037..4534)
                     /gene="paaD"
                     /locus_tag="ACHKNB_RS13440"
                     /old_locus_tag="pgaptmp_002688"
     CDS             complement(4037..4534)
                     /gene="paaD"
                     /locus_tag="ACHKNB_RS13440"
                     /old_locus_tag="pgaptmp_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HGU4196175.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(4549..5295)
                     /gene="paaC"
                     /locus_tag="ACHKNB_RS13445"
                     /old_locus_tag="pgaptmp_002689"
     CDS             complement(4549..5295)
                     /gene="paaC"
                     /locus_tag="ACHKNB_RS13445"
                     /old_locus_tag="pgaptmp_002689"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HGU4196176.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(5304..5591)
                     /gene="paaB"
                     /locus_tag="ACHKNB_RS13450"
                     /old_locus_tag="pgaptmp_002690"
     CDS             complement(5304..5591)
                     /gene="paaB"
                     /locus_tag="ACHKNB_RS13450"
                     /old_locus_tag="pgaptmp_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HGU4196177.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(5603..6532)
                     /gene="paaA"
                     /locus_tag="ACHKNB_RS13455"
                     /old_locus_tag="pgaptmp_002691"
     CDS             complement(5603..6532)
                     /gene="paaA"
                     /locus_tag="ACHKNB_RS13455"
                     /old_locus_tag="pgaptmp_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HGU4196178.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            6817..8862
                     /gene="paaZ"
                     /locus_tag="ACHKNB_RS13460"
                     /old_locus_tag="pgaptmp_002692"
     CDS             6817..8862
                     /gene="paaZ"
                     /locus_tag="ACHKNB_RS13460"
                     /old_locus_tag="pgaptmp_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HGU4196179.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            9110..11383
                     /gene="tynA"
                     /locus_tag="ACHKNB_RS13465"
                     /old_locus_tag="pgaptmp_002693"
     CDS             9110..11383
                     /gene="tynA"
                     /locus_tag="ACHKNB_RS13465"
                     /old_locus_tag="pgaptmp_002693"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HGU4196180.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVDDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(11441..12940)
                     /gene="feaB"
                     /locus_tag="ACHKNB_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /pseudo
     CDS             complement(11441..12940)
                     /gene="feaB"
                     /locus_tag="ACHKNB_RS13470"
                     /old_locus_tag="pgaptmp_002694"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
     gene            13176..14081
                     /gene="feaR"
                     /locus_tag="ACHKNB_RS13475"
                     /old_locus_tag="pgaptmp_002695"
     CDS             13176..14081
                     /gene="feaR"
                     /locus_tag="ACHKNB_RS13475"
                     /old_locus_tag="pgaptmp_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HGU4196181.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(14253..14582)
                     /locus_tag="ACHKNB_RS13480"
                     /old_locus_tag="pgaptmp_002696"
     CDS             complement(14253..14582)
                     /locus_tag="ACHKNB_RS13480"
                     /old_locus_tag="pgaptmp_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HGU4196182.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(14587..14772)
                     /locus_tag="ACHKNB_RS13485"
                     /old_locus_tag="pgaptmp_002697"
     CDS             complement(14587..14772)
                     /locus_tag="ACHKNB_RS13485"
                     /old_locus_tag="pgaptmp_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HGU4196183.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(14769..17408)
                     /locus_tag="ACHKNB_RS13490"
                     /old_locus_tag="pgaptmp_002698"
     CDS             complement(14769..17408)
                     /locus_tag="ACHKNB_RS13490"
                     /old_locus_tag="pgaptmp_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HGU4196184.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            17616..18605
                     /gene="ldhA"
                     /locus_tag="ACHKNB_RS13495"
                     /old_locus_tag="pgaptmp_002699"
     CDS             17616..18605
                     /gene="ldhA"
                     /locus_tag="ACHKNB_RS13495"
                     /old_locus_tag="pgaptmp_002699"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4196185.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            18716..19138
                     /gene="hslJ"
                     /locus_tag="ACHKNB_RS13500"
                     /old_locus_tag="pgaptmp_002700"
     CDS             18716..19138
                     /gene="hslJ"
                     /locus_tag="ACHKNB_RS13500"
                     /old_locus_tag="pgaptmp_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HGU4196186.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(19135..19401)
                     /locus_tag="ACHKNB_RS13505"
                     /old_locus_tag="pgaptmp_002701"
     CDS             complement(19135..19401)
                     /locus_tag="ACHKNB_RS13505"
                     /old_locus_tag="pgaptmp_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HGU4196187.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            19675..23199
                     /gene="nifJ"
                     /locus_tag="ACHKNB_RS13510"
                     /old_locus_tag="pgaptmp_002702"
     CDS             19675..23199
                     /gene="nifJ"
                     /locus_tag="ACHKNB_RS13510"
                     /old_locus_tag="pgaptmp_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HGU4196188.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         5727 a         6098 c         5816 g         5692 t
ORIGIN      
        1 tctttctttt cctggtggta gaaaagaaag cattgctgag cgtctgaaaa ggctgattgg
       61 ctcccgcagc gtcagagctg cagcaagaga ttgggggctt tcattttcaa cactaaacaa
      121 ttatctgtct cgtggcactg agccatcctt aagtgtcgcc cttcagattg cgtctatcga
      181 aggagtaagt gttgagtggc tatgtggatt cagtgatgca tcacaagtga atcggtgtga
      241 gatcaaggaa aatcaagatg aaagcaagaa gataattatg acgattatca gcgcacttga
      301 tgaaaaagac ttagagcagc tatcgaaaaa tttggttctg aatggagcca agtacttagc
      361 tcggttgtta gagcctgaaa atcaagatct aatccgctta gaaggaagga aaagaatagc
      421 tgcattacag cttgaaggta tgtctgacga acgcgtcaga gagattttgg aagaaactga
      481 gagaagtcgc aaatctaacg cggtagaacc aaaagcgggt taatagcatt agcccgcatt
      541 ttgcactata tcgagtattt cgcattgttt ggaacgcctt aaaagactaa aaccacagta
      601 tcaaatgagt tgccgatttt ataataaatg actaattatt gcacaatcgg tatcaaatag
      661 accttatccg cacatctagc catcacctgt tttatttcag tatcttacag gtgatttcac
      721 ctcctttcac taaacccctt gtcggtatca aatgattcac ctggttatat gcagcaacct
      781 gctcttccag tgcccgccag gcctcggaat aataaggacg cgcaacacgg ttaacgataa
      841 atccgggggt cgaatgacag cgcactggct gcttacccca actcaatgtc agttcacaca
      901 actgctcgac aacttccgct gccgttgcca gcccactgac cacctccacc aacttcatca
      961 ccggtgccgg gttaaaaaaa tgcagccccg caacacgttc aggatttttt acctccgcag
     1021 caatcgcggt tatagagatt gacgaagtgt tagtggtcaa tagcgtttgt ggcgggcaaa
     1081 cttccgccag ttgtgcaaag agcgcttttt taacttccag acgttcagac gccgcttcaa
     1141 taaccagatt cgctgctgcc agcgcgtgaa tatcggtcac cgggatcagg cgtttcaatg
     1201 tgcgttcaca ggtttcagcg gtcagttttc cccgcgtcac gcgtgaattt agccgcgcgt
     1261 gtatcccgtc gattgcgcgg gtcagcgctt cagcagaaat gtcatacagt aaaacctgat
     1321 gtccatgact ggcggcaact tcagcaatgc ctgcccccat ggtgccgctc ccaatcactg
     1381 ccacagtttg cacatttatc atcatgctat ttccccgtga actgcggtga gcgtttagcc
     1441 aggaacgcac tgacaccttc acgataatct gcgctccgtc cggcaagtcg ctgatagtca
     1501 cgttccagat ccagttgcgt atcgagcgta ttggtttcag cgctatttat cgcttgcttg
     1561 ataagtccaa gaccaaatgt cggttgtgtc gccagatgcc gtgccagctg ttgcgcggta
     1621 tctgccagcg tttcatcatc aacaacctgc cagatcatcc cccattcgtg cgcctgttca
     1681 gcactcagtt gattccccag cagtgccagc cccatggcgc gcgctcgccc agcaacgcgt
     1741 ggcagtaacc aggttccacc gcaatcgggt actaaaccta acttactaaa cgccatgacg
     1801 aattttgctg aacgggcagc aataacgatg tcgcatccca gtgccagtgt tgcgccagcc
     1861 cctgccgcca cgccattgac tgcacagatc accggttttg gcagttttgc caggcgacgt
     1921 accagtgggt tatagaaacg ttcaactgac atccctaaat ccggtgcggg gcctgtggga
     1981 tcaacgttac gatcgttaag atcctgacca gcacaaaatc cgcgcccggc acctgtaagt
     2041 aacaggcaac ggatagtgtc gtcgcgctcg acctgtttca ggcactctgc cagttgtgcg
     2101 tgcatctcat cattaaaact gttcaggcgt tccgggcggt tgagcgttag tgtcatcacg
     2161 cccttttcta catgactgag gatgaattcc atcattagcg tcctttaaag tcgggcgtgc
     2221 gtttttgcaa gaaagcggag atgccttcat gacgatcttc tgttgccgcc agcaaggtga
     2281 ataactgtcg ctcctgggca agtccggctt gcaaagccac ttcctgcgac tggcgcagcg
     2341 cttgctttgc cgcttgtaag gccagcggcg agtgacgtgc cattttcgac gccagctgta
     2401 aggcgtattc gagggttaaa tcgctgggga agacgtcgct aaccagcccg gcctgctgtg
     2461 cttgctgagc ggtgatactt tctccgctca gcaccatttt gctggctaac gatttaccgg
     2521 cactacggat taaacgttgc gttcctcctg cgcctggcat gatgccgaga gtgatttccg
     2581 gcaaaccaaa acgcgcgttc tctccggcaa ccaccacatc gcacaacaat gccagttcgc
     2641 aacccgcacc aagcgcgtaa ccgttgacgg ctgcgatgag aggtttgttg aaggcctgca
     2701 atcgcgccca tagctgcggg cgtgtatcgt ttaaggtggc cgcgagatct ttttctgcca
     2761 tttcgttgag atcggcccca gcggcaaaaa agcgtgcatt accggtaatc acacagaccg
     2821 aaatgctgct atcggtagcc gcagcttcca gttcatttac cagttgcgtc agaagggcat
     2881 tatttagcgc attacgtgcg gcgggacggt taagggtcag caacaatact tgttgctgac
     2941 ggctgacgat cagttcgctc atgccatccc cttcgcgtca aagtcaacca ccacatcgct
     3001 ggtcagcggc agtgcctggc aactcaacac atagcctgcg gccagttcat ccggttccag
     3061 actgtaattg gtttccatcg ccactttgcc acgcagcact ttgcatttgc aggtcgcaca
     3121 gacgccgcct ttgcaggcat agggcagatc agccccctgg cgcaatgccg catcgagaat
     3181 gctttcatcg tcggcattaa gcacgatttc ccgatcccgc ccatcctgac gtacagtcac
     3241 tttttgtccg tcactttgca cgttaacgct acgtttgacg cgcgtgccag gcgtattaaa
     3301 ccgctccaga tgaatggttt tatctggcat tcccagtgct tttaaggcgg cttccgtctc
     3361 atccatcatc gccgccgtac cacaaataaa tgcctcatca taaagacgaa aattaatgag
     3421 cgaggcccca agtgactgta atttttcacc gtcaatacgc ccgtgaagca gatcgctgtc
     3481 gagggtttcc tgactgaaaa tgcacaacaa ctgtaaacgc tgaggatatt tgtctttcag
     3541 gtctgccagt gcctggcgaa acatcatgct ctggctggta cggttaccgt agatcagggt
     3601 gaactgactt tcaggctcgg tttgtaaagt ggtggcgata atcgccagca ttggcgtaat
     3661 acctgatcct gctgcaatag ccagatagcg gccctggcgt tcggcctgtg gctgatagcc
     3721 gaaatgcccc tgcggcacca tgacctccag cgtcataccc tggcggatgt gttcgcgggc
     3781 atagcgggag aaacgtccgc cttcaatggc tttcaccgcc acactaattt cgccaggcag
     3841 atagctgcgg caaatggagt aacaacggcg taattcttca ccatcaaagc tggcttttaa
     3901 ggtcaaatgt tgaccggggc gaaagcgata cgcctcctgc aaaggctggg gcaccgcaaa
     3961 ggtaatggtc accgcatcac gggtttccgg ctccactttt gccaccgtta aggaatgaaa
     4021 cgttgtcatg gcatcctcaa atacatttga aataatcgaa aggttcgcgg caactatcgc
     4081 agcgatacaa tgctttgcag gccgtggaac caaattcact gataagtgtg gtatggacgc
     4141 tggcgcagcg cgggcaacgt acttctggcg gcaaatgggc atggcaactg tgtccggcgg
     4201 gcgggctaat gccatactgt cgcagacgct cacgggcatc gggggtcatc cagtcggtgg
     4261 tccatgccgg gtcgagttgc agcacaacct gaacgggggt aaacccgtgg gttgtcattg
     4321 cctcacgtat cgcgccaatc aaatgttccg ttgccggaca accggaataa gtcggcgtaa
     4381 agccgatcac ccatccttct cccatctgtg tcacattacg caccatgcct aaatcggtaa
     4441 tggtcagcac cgggatctcc gggtccggga tctggctgag tagcgcccat atctcatgaa
     4501 cctgtggcgg tgcaatggtg gccagacgtt gcatacccat ctcctctgtt accattgctg
     4561 accgggcaag acacgctgga gatactgcat ttctgccagc atgggtccaa ggtgttcggt
     4621 atgcagtcct tttttaccgc cagtgcgata cgcctgctct tgcggtacgt tcaatgtggc
     4681 ttcgttgatt ccggcaaaaa cttcggcttc ccacgctgcg cgtaaagtgc gtggatcaac
     4741 cgcaataccc tcttcactca gtgcaatatc aatctcgtcg gcatcgaaca gttcggcagt
     4801 aaaacgccac aacttgtcga tggcctgctg catcttttgc cctgatacgt cagtaccatt
     4861 gcccagtcgc tccagccagc cacgactaaa acgcaggtga taacgcgctt ctttaattgc
     4921 tttggcagaa atcgccgcca gttgcggatc acggctttcc atcagacggg taaagagcgc
     4981 cacatgccat gcatcgatga aatactggcg tgcaatggtg tcggcaaaat tgccgtttgg
     5041 ttgttcaacc aacaataagt tgctgaactg gcgctcgtct cgggtgaagg ccagggtatc
     5101 ttcatcacct tctcccgcta attcagcggc atacgataag aagttacgtg cctgacctaa
     5161 taaatcgagg ccaatgtttg ccagtgcgag atcgatttcc agttccggtg cgtgaccgca
     5221 ccattccccc aaccgctggg agagcaccag acagttatcg cccaggcgca aggtgtaagc
     5281 cgttaactga ttcatttccg acctcacatg tgctcaatgc catcagggat ggtgtaaaac
     5341 gttggatggc gatagacctt gctttcagcc ggatcaaaaa attcaccgcg ttcttccggt
     5401 tgcgaggcaa caatttcact cgccttcacc acccaaattg aacatccttc gctacgacgg
     5461 gtgtaagcat cacgggcatt ttccagtgcc atccgctcat cggcagcatg taaactgcca
     5521 acatggcggt gtgataagcc ctgtttgcca cgcacgaaaa cttcgtataa cggccagtaa
     5581 acattactca ttttgaatct ccttatgcga ccttacgggc aagttgtttt tgtgcatggg
     5641 ccagcgctgc ttcccgtacc caggtacctt cttcccaggc tttacgtttg gcgtcgagcc
     5701 tctcctgatt acaaattccg cgaccgttaa ttacttcgtt aaactcctgc cagtcgatct
     5761 caccaaagcg gtagtgaccg ctttcagtgt caaaatgcag atccgggtca ggaacggtca
     5821 taccgagcat ttcaacctgt ggaatggtgt tatccacgaa acgctggcgg agttcgtcgt
     5881 tggtgaaacg tttgattttc caggcgagac ttctggcgct gtttggcgag ttatcatcgt
     5941 ttggcccgaa catcattaag gctggccacc agaaacggtt aatggcgtct tgcaacatct
     6001 gcttttgagc ttcgctgccc tgcgcgagtg ccatgcaggc ttcaaacccc tgacgctggt
     6061 gaaaactctc ttctttgcag attttcacca ttgctctggc atacgggcca taagaagtgc
     6121 gacataacgc cacctgattg acgatcgctg cgccatccac cagccagccg ataacgccga
     6181 tatcagccca actcaatgtc ggataattaa agatggagga atatttcatc cgtccgtcga
     6241 gcattttttg gtagatgtct tctctggcgc agcccagtgt ttcggcggcg ctatagagat
     6301 acagaccatg accggcttca tcctgcactt tggccaacag aatggcttta cgccgcaagg
     6361 ttggtgcgcg agtgatccag ttaccttcag gcaacatacc aacaatctcg gagtgcgcat
     6421 gctgcccaat ctggcggatc agtgtctttc ggtaagcatc gggcatccag tcctgtggct
     6481 cgatagccgt ttcctgggct atccgttgct caaagcgttc ttcttgggtc acgtcatcac
     6541 ctttacgatt ccagttttcg tgaattaatt aaaaacacga atcacattgt tttgtgaaag
     6601 ttacacaatg gttaactaaa atcacaaatt ttgtatgctt gaactgtgac ggatttcgca
     6661 ccctattttt ataaatcatt atttatcagt gcattaatgt tttccgcagt tgctatacag
     6721 atcgcatagt taacatttcg ttaaaagaac ccttgctttt tatgattcgc gatttaacta
     6781 ttagcaacag aaatgtgaaa catctggaga gtagcgatgc agcagttagc cagtttctta
     6841 tccggtacct ggcagtctgg ccggggccgt agccgtttga ttcaccacgc tattagcggc
     6901 gaggcgttat gggaagtgac cagtgaaggt cttgatatgg cggctgcccg ccagtttgcc
     6961 attgaaaaag gtgcccccgc ccttcgcgct atgaccttta tcgaacgtgc ggcgatgctt
     7021 aaagcggtcg ctaaacatct gctgagtgaa aaagagcgct tctatgctct ttctgcgcaa
     7081 acaggcgcaa cgcgggcaga cagttgggtt gatattgaag gtggcattgg gacgttattt
     7141 acttacgcca gcctcggtag ccgggagctg cctgacgata cgctgtggcc ggaagatgaa
     7201 ttgatcccct tatcgaaaga aggtggattt gccgcgcgcc atgtactgac ctcaaagtca
     7261 ggcgtggcag tgcatattaa cgcctttaac ttcccctgct ggggaatgct ggaaaagctg
     7321 gcaccaacgt ggctgggcgg aatgccagcc atcatcaaac cagctaccgc gacggcccaa
     7381 ctgactcagg cgatggtgaa atcaattgtc gatagtggtc ttgttcccga aggcgcaatt
     7441 agtctgatct gcggtagtgc gggcgacctt ttggatcatc tggacagcca ggatgtggtg
     7501 actttcacgg ggtcagcggc gaccggacag atgctgcgag ttcagccaaa tatcgtcgcc
     7561 aaatctatcc ccttcactat ggaagctgat tccctgaact gctgcgtact gggcgaagat
     7621 gtcaccccgg atcaaccgga gtttgcgctg tttattcgtg aagttgtgcg tgagatgacc
     7681 acaaaagccg ggcaaaaatg tacggcaatc cggcggatta ttgtgccgca ggcattggtt
     7741 aatgctgtca gtgatgctct ggttgcgcga ttacagaaag tcgtggtcgg tgatcctgct
     7801 caggaaggcg tgaaaatggg cgcactggta aatgctgagc agcgtgccga tgtgcaggaa
     7861 aaagtgaaca tattgctggc tgcaggatgc gagattcgcc tcggtggtca ggcggattta
     7921 tctgctgcgg gtgccttctt cccgccaacc ttattgtact gtccgcagcc ggatgaaaca
     7981 ccggcggtac atgcaacaga agcctttggc cctgtcgcaa cgctgatgcc agcacaaaac
     8041 cagcgacatg ctctgcaact ggcttgtgca ggcggcggta gccttgcggg aacgctggtg
     8101 acggctgatc cgcaaattgc gcgtcagttt attaccgacg cggcacgtac gcatgggcga
     8161 attcagatcc tcaatgagga atcggcaaaa gaatccaccg ggcatggctc cccactgcca
     8221 caactgttac atggtgggcc tggtcgcgca ggaggcggtg aagaattagg tggtttacga
     8281 gcggtgaaac attacatgca gcgaaccgct gttcagggta gtccgacgat gcttgccgct
     8341 atcagtaaac agtgggtgcg cggtgcgaaa gtcgaagaag atcgtattca tccgttccgc
     8401 aaatattttg aggagctgca accaggcgac agcctgttga ctccccgccg cacaatgaca
     8461 gaggccgata ttgttaactt tgcttgcctc agcggcgatc atttctatgc gcatatggat
     8521 aagattgctg ctgccgaatc tattttcggt gagcgtgtgg tgcatgggta ttttgtgctt
     8581 tctgcggctg cgggtctgtt tgtcgatgcc ggtgtcggtc cggtcattgc taattatggg
     8641 ctggaaaact tgcgctttat cgaacccgta aagccaggcg ataccatcca ggtgcgtctc
     8701 acctgtaagc gcaagacgct gaaaaaacag cgtagcgcag aagaaaaacc aacaggtgtg
     8761 gtggaatggg cggtagaggt attcaatcag catcaaaccc cggtggcgct gtattcaatt
     8821 ctgacgctgg tggccaggca gcacggtgat tttgtcgatt aatcggtgaa tgaagggcaa
     8881 cggcgaatag ttgccctttt atttcaccaa attttgtgac gttgtcacgt tatgcatgat
     8941 gtgtatctgt attttcaggg cacccactgt atgaaaagct ggcacacctg ccaaacaacc
     9001 tggcaggtgc aggcaatccc ctttacatca gtactgataa tgtgaacctg actaaaccgc
     9061 ccacagagcg cggttgctaa caagaacaca acatctgacg aggttaataa tgggaagccc
     9121 ctctctgtat tctgcccgta aaacagccct ggcgttggca gtcgccttaa gtttcgcctg
     9181 gcaagcgccg gtatttgccc acggtggtga agcgcatatg gtgccaatgg ataaaacgct
     9241 taaagaattt ggtgccgatg tgcagtggga cgactacgcc cagatcttta ccctgattaa
     9301 agatggcgcg tacgtgaaag tgaagcctgg tgcgcaaaca gcaattgtta atggtcagtc
     9361 tctggcactg caagtaccgg tagtgatgaa agacaataaa gcctgggttt ctgacacctt
     9421 tattaacgat gttttccagt ccgggctgga tcaaaccttt caggtagaaa agcgccctca
     9481 cccacttaat gcgctaactg cggacgaaat taaacaggcc gttgaaattg ttaaagcttc
     9541 cgcggacttc aaacccaata cccgttttac tgagatctcc ctgctaccgc cagataaaga
     9601 agctgtctgg gcgtttgcgc tggaaaacaa accggttgac cagccacgca aagccgacgt
     9661 cattatgctc gacggcaaac atatcatcga agcggtggtg gatctgcaaa acaacaaact
     9721 gctctcctgg caacccatta aagacgccca cggtatggtg ttgctggatg atttcgccag
     9781 tgtgcagaac attattaaca acagtgaaga atttgccgct gccgtgaaga agcgcggtat
     9841 tactgatgcg aagaaagtga ttaccacgcc gctgaccgta ggttatttcg atggtaaaga
     9901 tggcctgaaa caagatgccc ggttgctcaa agtcatcagc tatcttgatg tcgatgatgg
     9961 caactactgg gcacatccca tcgaaaacct ggtggcggtc gttgatttag aacagaaaaa
    10021 aatcgttaag attgaagaag gtgcggtagt tccggtgcca atgaccgcac gcccatttga
    10081 tggccgtgac cgcgttgctc cggcagttaa gcctatgcaa atcattgagc ctgaaggtaa
    10141 aaattacacc attactggcg atatgattca ctggcggaac tgggattttc acctcagcat
    10201 gaactcgcgc gtcggaccta tgatctccac cgtgacttat aacgacaatg gcaccaaacg
    10261 caaagtcatg tacgaaggtt ctctcggcgg catgattgtg ccttacggtg atcctgatat
    10321 tggctggtac tttaaagcgt atctggactc tggtgactac ggtatgggca cgctaacctc
    10381 gccaattgca cgtggcaaag atgcaccctc taacgcagtg ctcctgaatg aaaccatcgc
    10441 cgactacact ggcgtaccga tggagatccc tcgtgctatc gcggtatttg aacgttatgc
    10501 cgggccggag tataagcatc aggaaatggg ccagcccaac gtcagtaccg aacgccggga
    10561 gttggtggtg cgctggatca gtacagtggg taactatgac tacatttttg actggatctt
    10621 ccatgaaaac ggcactattg gcattgatgc cggtgctacg ggcatcgaag cggtgaaagg
    10681 tgttaaagcg aaaaccatgc acgatgagac ggcgaaagat gacactcgct acggcacgct
    10741 tatcgatcac aatatcgtgg gtactacaca ccaacatatt tataatttcc gcctcgatct
    10801 ggatgtagat ggcgagaata acagcctggt ggcgatggac ccggtggtaa aaccgaatac
    10861 tgccggtggc ccacgcacca gtaccatgca agttaatcag tacaacatcg gcaatgaaca
    10921 ggatgccgcg cagaagtttg atccgggcac gattcgtctg ttgagtaacc cgaacaaaga
    10981 gaaccgcatg ggcaatccag tttcctatca aattattcct tatgcaggtg gtactcaccc
    11041 ggtagcaaaa ggtgcccagt tcgcgccgga cgagtggatc tatcatcgtt taagctttat
    11101 ggacaagcag ctttgggtaa cgcgttatca tcctggcgag cgtttcccgg aaggcaaata
    11161 tccgaaccgt tctactcatg acaccggtct tggacaatac agtaaagata acgagtcgct
    11221 ggacaacacc gacgccgttg tctggatgac caccggcacc acacatgtgg cccgcgccga
    11281 agagtggccg attatgccga ccgaatgggt acatactctg cttaaaccat ggaacttctt
    11341 tgacgaaacg ccaacgctag gggcgctgaa gaaagataag tgattgtttc agacaaaaaa
    11401 acggcaccag gtgccgtttt ttacttatga gcgaaccaga ttaataccgt acacacaccg
    11461 acttagtttc acaccaaccg tccagccagt cggggccaaa atcacggccc gttcctgact
    11521 gcttcatccc accaaacggt aagttagcgt caattaaggt atggctgttt acccacaccg
    11581 tccctgcctg taagcgatcg ctatattcca gagcctggga gagattttgc gtccagacac
    11641 tggcagttaa gccatattcc gtgtcgtttg ccagttgtaa cgcctcttct ccatccgcta
    11701 ctcgcaccag gtttaccacc ggaccaaaca cctcttcacg agttaagcgc aatttagcat
    11761 cgggatttac caccagcgtt ggcgcaacat aatacccctc tccggctggt ccattcgacc
    11821 cgcgaatcag ctctgcttgc tgtgcctgcg catcgtcgag gaatgaacac actttgtcgc
    11881 agtgcgcacg agaaaccaaa gggttaatct gtgcaacagg tgacatcccc ggtcccactt
    11941 acaacgattt taccgcctgc tcaaatccac taaccagcgt gtcaaacaac ggcgcttcaa
    12001 tataaattcg cgaactggcg gcgcatactt gcccttgatt caggaagctt ccggtcatca
    12061 agccttcaat aacccattgc ggatcagcat cttttaatac aattgccggg tttttaccgc
    12121 ccagttccag cgttacacgc gttaagtgat cagcagcagt tctggcaata ccttttcccg
    12181 tcgcggttga accggtaaaa ctgattttcg caacatgagg atgtgacgtc agggccgcgc
    12241 cgcatacagc acctgacccg gtgacgacat taaaaacgcc atcagggata ccagcctcgc
    12301 tggccagttc cgccacgcgc aacatcgtca gtggcgtggt ttccgaaggc ttaatcacga
    12361 ttgaacagcc tgctgccagt gctggcatca ccttccacat accaatcatc aacggaaagt
    12421 tccatggcac aattcccgcc actacgccaa ccggctcttt acgcgtccag gcctgataac
    12481 gcgccccctg gggtaaggga atcgacaagt ccagcgtttt acccgcgatt ttggtcgtta
    12541 acccggcggt ataacgcatc cagttcagcg tacagcccac ttcaaaagca cgggaaatgg
    12601 caattgactt gccttgctcc agggtttcca gttgcgccag ctcctcactg tgctgttcca
    12661 ccagatcggc aaaacgtagc agaatacgtt cacgctctgc gggtaatcgc ccggcccagc
    12721 gacgcgagac aaaggcccgc caggcagaca tgactgcgtt atctacatcc gcttcgttgg
    12781 catcagcagt agacgcaatt tcttgcccgg tggccggatc aaagatcgcc aatcgttttt
    12841 cactttgtgc ggggcctgga cgaccatcaa tataaagacc gtgttgacga tcgagaaact
    12901 gttggacctg gcttaatact gctacatgcg gctctgtcat cacttttcct tattatttac
    12961 ccagtgtgat ttcagtgtac gcagcgacaa gaaaagtcac ttttttcgct gtgacattcg
    13021 ctttgtcagc tgtgacaagc tccgcaaatc gtgacaataa ccgcttatgt ctctatgcct
    13081 gagaagactt atttcctata gtcaggctca ttagcttcgt ttactgcaac acaaatgcaa
    13141 caataaaaat acatttcaca gagcgaaaac gtgccatgaa ccccgcagtg gataatgagt
    13201 ttcagcaatg gctttcccaa atcaatcagg tatgcggaaa ttttaccgga cgcctgctga
    13261 ctgagcgtta cacgggtgta ctggacacgc attttgccaa aggactaaag ctgagtaccg
    13321 tgacaaccag cggggtgaat ttatcccgca cctggcagga agtaaaaggc agcgacgatg
    13381 cctggtttta caccgttttt cagcttagtg gtcaggcaat aatggagcag gatgagcgtc
    13441 aggtgcagat tggcgctggc gatattacgt tactcgatgc ctcacgcccc tgttcgcttt
    13501 actggcagga gtcttctaaa cagatttcat tacttttgcc acgcactctg ctggaacaat
    13561 attttcccca tcaaaaacct gtctgcgcag aaagactgga cgctgactta cccatggtgc
    13621 aactcagtca tcgcctgtta caggagagca tgaataatcc ggcactttct gaaacagaaa
    13681 gtgaagctgc gctacaggcg atggtgtgtc tgctgcgccc ggtacttcat cagcgggaat
    13741 ctgttcaacc tcgtcgtgaa cgtcagtttc aaaaagtggt tacgttgata gacgataata
    13801 ttcgcgaaga gatattacgc ccggagtgga tagccggaga gacaggtatg tcagtacgta
    13861 gtttgtaccg aatgtttgcc gataaaggtt tggtagtcgc gcaatatatt cgtaaccgtc
    13921 gtctcgattt ttgtgcagat gcgattcgcc atgccgcaga tgatgaaaaa ctggcaggca
    13981 tcggctttca ttggggattt tctgaccaga gtcatttttc aacggtattt aagcaacgct
    14041 ttgggatgac gccaggcgag tatcgacgta aattccgcta actaattggc gttgtgtgta
    14101 ctgcaacgcc aattagttaa aacagatatt tgactatgca tttttcaggg cgaaaggcaa
    14161 tttgacagga gtttttgaat attcaccttg tttcaggaaa atagatcaat cgctttctcc
    14221 tgtagcgtta tcccgatatt gcggttttgc tattaaaatt ttcgcaccca tttaccgttt
    14281 atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc ggcgagtttc
    14341 gcgatatcat ctacagacac attattttgc tttgccagtt gttggtaact cgctttacgt
    14401 tcggcattaa tgtcggttac taatttctct gtttcagcat ccgttttcag cgcaaccaga
    14461 taaccataaa atgtttcacc tacccgcccc tgagttctgg cttcatccag agtcaatgcc
    14521 attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt tttcttcatc
    14581 atcgcctcag aaaagatcgc tacgagtttc aagcagctct tcgacatctt tgtctgcctt
    14641 gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt ccttgggcgc
    14701 agcgacttca atacgcggcg tacaggcagc cagcataaaa gatgacgtca acgcagccag
    14761 taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt tcccggcaga
    14821 cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact gcgccacagc
    14881 gtaaaaatat tctcttcatg atagtaatta aggtttaccg taccgctttt accatcaacg
    14941 cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc taagttaatt
    15001 tttgtcgatg accggctaat ttccatatag cgcaaccagt taattgctga acccgcagtc
    15061 atattgtctt tcaccaccgc atctgcggtg tctttatcca gccgcaaggt cattggcccg
    15121 ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca taacggcaat
    15181 gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct aaccagttcg
    15241 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
    15301 ttcatcagta cgttaccgcc taacacatcg acgctaacat cagtcaacag caagggttct
    15361 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
    15421 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
    15481 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
    15541 gcactaccgc ctttcaacac gccgtgtcct cccgcgcgga atccttgttc aggcgcagca
    15601 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
    15661 accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc gcgcagacga
    15721 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
    15781 ttaaagagga aatacgtcgg atcgcgccca tcgacgctaa attttaaggt tgatggcggt
    15841 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
    15901 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
    15961 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagaggtc ggtcagcgta
    16021 atcgtgctgt catgccattc accttttcct gcgacatccc aggtggcatt catcggtgta
    16081 aaactgcctt ttccccagta gcgccactgc cagcggcccg cgtcagggag aaaatcattc
    16141 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
    16201 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
    16261 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
    16321 tcaaaggtca gcgttgggtc agacagactt ccacttagct gcgcaggtaa acgggcatat
    16381 aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagctg actgttatcc
    16441 atgcttaatt ttcctgggcc aaaatttagc accgcgttgc ctttacccgc ttgcccctgg
    16501 gtcagcacac tcagtcgtcc gctgatcaga gcgttctcaa gccctgcctg ccagttgtcg
    16561 actttgacac ccagtcggcc actcaaagga aaacctgcat acggccaact ccagcgacca
    16621 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
    16681 ttatcccgtg ccagcacaat caattgcccg ctattttcct gccagtccag ctctgcatcc
    16741 accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact tacaggaagt
    16801 ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
    16861 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
    16921 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
    16981 tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat ccaggtgtta
    17041 ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
    17101 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
    17161 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
    17221 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
    17281 cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc gagcgtcatc
    17341 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
    17401 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
    17461 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
    17521 tttttcaata tcgccatagc tttcaattat atttgaaatt ttgtaaaata tttttagtag
    17581 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
    17641 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
    17701 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
    17761 gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg
    17821 ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag
    17881 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
    17941 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
    18001 acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa acggcaggcg
    18061 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
    18121 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
    18181 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
    18241 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
    18301 tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa
    18361 atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctattctttg
    18421 aagataaatc caacgacgta attcaggatg acgtattccg tcgcctgtct gcctgccaca
    18481 acgtgctatt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
    18541 agactacgct gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg
    18601 tttaatcttg ccgctcccct gcattccagg ggagctgatt cagataatcc ccaatgacct
    18661 ttcatcctct attcttaaaa tagccctgag tcagaaactg taattgagaa ccacaatgaa
    18721 gaaagtagcc gcgctcgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
    18781 aattgctgta acgccagaac agttacagca tcatcgtttt gtgctggaaa gcgtaaacgg
    18841 taagcccgtg accaacgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
    18901 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
    18961 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
    19021 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
    19081 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
    19141 gctgccacag ctcccggcgg caagtgactg ttcactacag cgtttgccgt tgggtaatgc
    19201 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
    19261 tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc
    19321 cgcagtcgcc tgctgaacag gttcactact acacgccgac aacaataaag cggcacaccc
    19381 tacccaaaac gctgctcgca tcttttcttc ctctgatctt caagccaaac gacaccgcca
    19441 taaataatag gcagcacaga gggcgacgtc gagagctgtc ctgcgcgttg ccccgcattt
    19501 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg
    19561 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aggattacta
    19621 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
    19681 actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc
    19741 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
    19801 ggcttaaaga acgtttgggg agacacacca cgcgtagttg aaatgcagtc ggaagcgggt
    19861 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
    19921 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
    19981 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactttctat ttttggcgat
    20041 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc ggctaacgtc
    20101 caggaagcgc aagattttgc tctcatttcg cacatcgcaa cgctgaagag ccgcgtgcca
    20161 tttattcatt tctttgatgg tttccgcacg tcccacgaaa tcaataaaat tgtcccgctg
    20221 gccgatgaca ctattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
    20281 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
    20341 cagtctcgcg aagccaccaa cccatggtac gacgcggtct atgaccatgt tgaacaggcg
    20401 atgaatgatt tctctgccgc gacaggtcgt cagtatcagc cgtttgaata ttacgggcat
    20461 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
    20521 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg cctgtaccgt
    20581 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
    20641 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtaatgacc
    20701 gcactggcag aagcctttaa taatggcgag cgcgaaactc tgccccgtgt cattggtggg
    20761 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
    20821 ctcaacgcgg ctaaaccgaa agcgcgcttt acggttggta tttacgatga tgtgaccaat
    20881 ctgtcactgc cgttgccgga aaacaccctg ccaaactcgg cgaaactgga agccttgttt
    20941 tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt
    21001 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc
    21061 ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta tctcatttcc
    21121 caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca gatggctgag
    21181 cgtttaaaac ctggcggcat tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg
    21241 tcgcgcttgc cgcaggaagt tcaggccgtg ctaaatcaga aaaaagcgcg cttctatgtg
    21301 attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
    21361 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
    21421 cagggtgcga ttgccaaaag ttacagtagc aaaggccagg atctggtgga acgcaactgg
    21481 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
    21541 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
    21601 accgcagcga tgctcgcagg gcttggtgat gccctccccg tttcggcgct gccgccagac
    21661 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca atatcgccga agagatcccc
    21721 atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg cccacactca
    21781 gctattcgcg caaaagtggt accgcctgaa gcgatggaaa acgcccctgc cagcctgcat
    21841 tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggctccg
    21901 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
    21961 ccagagatta aagccatcaa tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc
    22021 aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga acgtattgat
    22081 attcgtacat cgcagctgat tacaccgctg tttgaatatt caggtgcttg ctccggttgt
    22141 ggcgagacgc cgtatattaa attactgact cagctctatg gcgaccggat gttgatcgct
    22201 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
    22261 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
    22321 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
    22381 caatttgccg ataaaatccc ggcggaatta ctgacagcgt tgaaatcaga cgctacgcca
    22441 gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt tgccgaagca
    22501 catgaactgc tgcgtgacgc agatgcactg gtggaaaaat caatctggct gattggtggc
    22561 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
    22621 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
    22681 aaagcgacac cgctgggtgc agtaactaag ttcggcgagc acggcaaacg taaagcgcgt
    22741 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
    22801 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccggggcca
    22861 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc actcagccat
    22921 gaccagatgc gccaactcac agctaccggc ttctggccgc tatatcgctt cgatcctcgt
    22981 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
    23041 gaagaaacgt tacttcacga gcaacgtttc cgtcgtctga attcgcagca gccagaagtg
    23101 gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca
    23161 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
    23221 caattctaaa aaaaaagccc ggacgactgt tcgggcttgt ctttttatat gttggaaaat
    23281 cagtggcaat gcaatggccc aacagaaaat aaaatctgtg acaataaagg cat
//
LOCUS       DAZFVO020000076        23330 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFVO020000076 DAZFVO020000000
VERSION     DAZFVO020000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.076"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..594)
                     /locus_tag="ACHKNB_RS13515"
                     /old_locus_tag="pgaptmp_002703"
     CDS             complement(<1..594)
                     /locus_tag="ACHKNB_RS13515"
                     /old_locus_tag="pgaptmp_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4196189.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIG"
     gene            complement(612..1544)
                     /locus_tag="ACHKNB_RS13520"
                     /old_locus_tag="pgaptmp_002704"
     CDS             complement(612..1544)
                     /locus_tag="ACHKNB_RS13520"
                     /old_locus_tag="pgaptmp_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4196190.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(1544..2464)
                     /gene="ycjY"
                     /locus_tag="ACHKNB_RS13525"
                     /old_locus_tag="pgaptmp_002705"
     CDS             complement(1544..2464)
                     /gene="ycjY"
                     /locus_tag="ACHKNB_RS13525"
                     /old_locus_tag="pgaptmp_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4196191.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2602..3501
                     /gene="pgrR"
                     /locus_tag="ACHKNB_RS13530"
                     /old_locus_tag="pgaptmp_002706"
     CDS             2602..3501
                     /gene="pgrR"
                     /locus_tag="ACHKNB_RS13530"
                     /old_locus_tag="pgaptmp_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HGU4196192.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3838..5451
                     /gene="mppA"
                     /locus_tag="ACHKNB_RS13535"
                     /old_locus_tag="pgaptmp_002707"
     CDS             3838..5451
                     /gene="mppA"
                     /locus_tag="ACHKNB_RS13535"
                     /old_locus_tag="pgaptmp_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HGU4196193.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(5502..6533)
                     /gene="ynaI"
                     /locus_tag="ACHKNB_RS13540"
                     /old_locus_tag="pgaptmp_002708"
     CDS             complement(5502..6533)
                     /gene="ynaI"
                     /locus_tag="ACHKNB_RS13540"
                     /old_locus_tag="pgaptmp_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HGU4196194.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            6777..7034
                     /gene="ynaJ"
                     /locus_tag="ACHKNB_RS13545"
                     /old_locus_tag="pgaptmp_002709"
     CDS             6777..7034
                     /gene="ynaJ"
                     /locus_tag="ACHKNB_RS13545"
                     /old_locus_tag="pgaptmp_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HGU4196195.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(7084..8034)
                     /gene="uspE"
                     /locus_tag="ACHKNB_RS13550"
                     /old_locus_tag="pgaptmp_002710"
     CDS             complement(7084..8034)
                     /gene="uspE"
                     /locus_tag="ACHKNB_RS13550"
                     /old_locus_tag="pgaptmp_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HGU4196196.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(8186..8938)
                     /gene="fnr"
                     /locus_tag="ACHKNB_RS13555"
                     /old_locus_tag="pgaptmp_002711"
     CDS             complement(8186..8938)
                     /gene="fnr"
                     /locus_tag="ACHKNB_RS13555"
                     /old_locus_tag="pgaptmp_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HGU4196197.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(9133..9648)
                     /gene="ogt"
                     /locus_tag="ACHKNB_RS13560"
                     /old_locus_tag="pgaptmp_002712"
     CDS             complement(9133..9648)
                     /gene="ogt"
                     /locus_tag="ACHKNB_RS13560"
                     /old_locus_tag="pgaptmp_002712"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGU4196198.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(9659..11185)
                     /gene="abgT"
                     /locus_tag="ACHKNB_RS13565"
                     /old_locus_tag="pgaptmp_002713"
     CDS             complement(9659..11185)
                     /gene="abgT"
                     /locus_tag="ACHKNB_RS13565"
                     /old_locus_tag="pgaptmp_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HGU4196199.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDVLESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(11219..12664)
                     /gene="abgB"
                     /locus_tag="ACHKNB_RS13570"
                     /old_locus_tag="pgaptmp_002714"
     CDS             complement(11219..12664)
                     /gene="abgB"
                     /locus_tag="ACHKNB_RS13570"
                     /old_locus_tag="pgaptmp_002714"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HGU4196200.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(12664..13974)
                     /gene="abgA"
                     /locus_tag="ACHKNB_RS13575"
                     /old_locus_tag="pgaptmp_002715"
     CDS             complement(12664..13974)
                     /gene="abgA"
                     /locus_tag="ACHKNB_RS13575"
                     /old_locus_tag="pgaptmp_002715"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HGU4196201.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            14150..15058
                     /gene="abgR"
                     /locus_tag="ACHKNB_RS13580"
                     /old_locus_tag="pgaptmp_002716"
     CDS             14150..15058
                     /gene="abgR"
                     /locus_tag="ACHKNB_RS13580"
                     /old_locus_tag="pgaptmp_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4196202.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            15388..15951
                     /gene="smrA"
                     /locus_tag="ACHKNB_RS13585"
                     /old_locus_tag="pgaptmp_002717"
     CDS             15388..15951
                     /gene="smrA"
                     /locus_tag="ACHKNB_RS13585"
                     /old_locus_tag="pgaptmp_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HGU4196203.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(15972..17204)
                     /gene="dgcM"
                     /locus_tag="ACHKNB_RS13590"
                     /old_locus_tag="pgaptmp_002718"
     CDS             complement(15972..17204)
                     /gene="dgcM"
                     /locus_tag="ACHKNB_RS13590"
                     /old_locus_tag="pgaptmp_002718"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HGU4196204.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            17398..17445
                     /gene="ydaN"
                     /locus_tag="ACHKNB_RS13595"
                     /old_locus_tag="pgaptmp_002719"
     CDS             17398..17445
                     /gene="ydaN"
                     /locus_tag="ACHKNB_RS13595"
                     /old_locus_tag="pgaptmp_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HGU4196205.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            17459..18442
                     /gene="zntB"
                     /locus_tag="ACHKNB_RS13600"
                     /old_locus_tag="pgaptmp_002720"
     CDS             17459..18442
                     /gene="zntB"
                     /locus_tag="ACHKNB_RS13600"
                     /old_locus_tag="pgaptmp_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HGU4196206.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            18717..18890
                     /gene="ynaL"
                     /locus_tag="ACHKNB_RS13605"
                     /old_locus_tag="pgaptmp_002721"
     CDS             18717..18890
                     /gene="ynaL"
                     /locus_tag="ACHKNB_RS13605"
                     /old_locus_tag="pgaptmp_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HGU4196207.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            18920..20293
                     /gene="dbpA"
                     /locus_tag="ACHKNB_RS13610"
                     /old_locus_tag="pgaptmp_002722"
     CDS             18920..20293
                     /gene="dbpA"
                     /locus_tag="ACHKNB_RS13610"
                     /old_locus_tag="pgaptmp_002722"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HGU4196208.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGVDIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(20422..21357)
                     /gene="ttcA"
                     /locus_tag="ACHKNB_RS13615"
                     /old_locus_tag="pgaptmp_002723"
     CDS             complement(20422..21357)
                     /gene="ttcA"
                     /locus_tag="ACHKNB_RS13615"
                     /old_locus_tag="pgaptmp_002723"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HGU4196209.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(21533..21967)
                     /gene="uspF"
                     /locus_tag="ACHKNB_RS13620"
                     /old_locus_tag="pgaptmp_002724"
     CDS             complement(21533..21967)
                     /gene="uspF"
                     /locus_tag="ACHKNB_RS13620"
                     /old_locus_tag="pgaptmp_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HGU4196210.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(22108..23241)
                     /gene="ompN"
                     /locus_tag="ACHKNB_RS13625"
                     /old_locus_tag="pgaptmp_002725"
     CDS             complement(22108..23241)
                     /gene="ompN"
                     /locus_tag="ACHKNB_RS13625"
                     /old_locus_tag="pgaptmp_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HGU4196211.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLINGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         6001 a         5590 c         5672 g         6067 t
ORIGIN      
        1 acctatatta tcaccaatgt gtttctcagg tttatcgatg atatctgtga ttaatgctgc
       61 gacactctta cgtgatacga tcgtaccttt gaatggttca ttgcgtgagg taagctcata
      121 atcaataata tcttcatcag tcagccaggc agggcgaaga attgtatatt ccagtccaga
      181 agcctcaatt gcatccgcag ccctgcggaa tggttttagg ggctcaccaa taactgcatt
      241 attccattcg ccaaattttc ctggaacttc atcataaata ccgagggaaa gaacaaagat
      301 cagacgttta acatcacagg ctttcatcgc agcgatcact gaatttgcct gaatatctaa
      361 atcttccccc gtaaggttgg catacacaac gtcctgtccc tgcatggcct gcttcagtgc
      421 tgcatgattc agtacatctc ccataataat cttgctatta gttggatagg gtttgtggat
      481 ttttgctggc tgacgggcaa ataaagtttg tttaatggtt tgtttatctg ccagttgatt
      541 aatcacatgg cgggctatct gaccgccagc accaagaatc agtacatttt tcataatatt
      601 tctcatgaaa cttatcccag atgggtgttg aaaaaaggaa taactttttc cagcgcctcc
      661 tgaactgctt ccggtttgtc atacagttca taatgtgaga aatcgggtaa tgaaactaac
      721 tgacgatcct gcgaaaccat cgcccggcca tagacttcca gtccatcacg ataagcaccg
      781 aaggcaccga ctttttcacc aactactacc atcaccggct gagtcagtaa cacttcagta
      841 aatgcgaagg catcccaggc cagcgttttc tgtgcatgtg agaacaacat ttttgtcgca
      901 cctcccggtt gttgcccgcg tggtgtttta tagtagtcag ttgcttcata aacatcgcgt
      961 tcggtcagtc catgggcttt tgctgcatct aggctggcgg gaagtaattc gttgatctgt
     1021 aattctccac cacgagcctc tttggttcgt tgcgacgcca ttgcattcaa tgcgccaatc
     1081 ggatcgtaat tgctgaaacc ttcccgaaaa agacgcccga tattaacacc cgtaatactc
     1141 actactgctt taattcgttt ttctgtcagc gcggcattaa tggcatagcc accaccccca
     1201 cacactccca gcacgccaat acgttcagca ttaacatatg gcagagttac tgcataatcg
     1261 ataacacggc taatatcctc tacgcgctgt gtcggatctt ctatccagcg aggctcccca
     1321 ccagattctc cctggaaact ggcgtcataa gcgagaacga gatagccttt ttcagccaat
     1381 gctttgccat agatattgcc agacgtctgc tccttacaac tgccaaaagg atggacgctg
     1441 ataatcgccg gatactgttt tgactcatca aaatttggtg gaaactgaat attggcagca
     1501 atatgccagg ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa
     1561 agaacggagc taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca
     1621 tatggtttgc gccttcaaca atgtgatagc gtttatcctg gcttgatgcc cgatcgtaca
     1681 ggtcgtcact catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag
     1741 tcaggtacac ttccgccata tggtaagcat cataggtaat aatctggtta aggctgcgca
     1801 aagtagcgta acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg
     1861 cctgacgcag ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg
     1921 catattctcc gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca
     1981 gggcatcaat agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga
     2041 ccgcactaac ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat
     2101 atcctgcacc ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg
     2161 ttgtcaggta atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact
     2221 gacgcggctc gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc
     2281 ccttttccgc cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg
     2341 ggtgagataa cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga
     2401 tcactgcaga caaagagatg gttggattat tgctgttagt gaagctgact ttattattca
     2461 tcattccgtt cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat
     2521 attgatcatt aagttgatta gacccaaaat catgattaga cctatcattt aaatgattaa
     2581 tagattgcat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc
     2641 gtcgttgcag aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca
     2701 gctttaagcc agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga
     2761 accacgcgca gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct
     2821 atgttgcatg acatagattc agccatggca tccctgagcg atctgcagaa ccgcccatcc
     2881 gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagcaatg
     2941 cgcacattcc tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg
     3001 accgatgtcg tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa
     3061 gatatgatcg ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat
     3121 tatttttctc gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt
     3181 aatttgtatc ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt
     3241 gaagttcgtg ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat
     3301 gctgcaattg atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt
     3361 aaagaaaaaa aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac
     3421 ctgtactatc cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg
     3481 aagtataaag gtgctgttta gcactacttg ctgatacatt aatttaattt ctctcttaac
     3541 gtattctcag ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga
     3601 tgctgatttt gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg
     3661 ttctatgtgc tttgccgcat tttggcgtcg attatttaca ctaaatctga tttaatatat
     3721 tgatacataa aacattttat gcttcctgtg tcactttttt gatggaagtt gtttgcattt
     3781 ctttaaggcg aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg
     3841 aagcactctg tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat
     3901 gctgcagaag ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt
     3961 aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc
     4021 attcgcgatc tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc
     4081 gttgcgactc agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac
     4141 gcaaaatggg cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcaacgt
     4201 ctggtggacc cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac
     4261 aacgcacagg cgattattga tggtaaagct acgcctgacc agcttggcgt caccgcagtt
     4321 gatgcccata ccttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc
     4381 gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg
     4441 aaacccggaa atctgatcgg caatggcgct tatgttctta aagaacgcgt agtcaatgaa
     4501 aaactggtcg tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa
     4561 gtgaccttcc tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat
     4621 attgatatca ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg
     4681 gggcaggttt atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa
     4741 gggccgacgg cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg
     4801 atgaccgaaa aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt
     4861 accgccggat ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat
     4921 gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg
     4981 acgcttttgt ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg
     5041 tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc
     5101 gatagccgta acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat
     5161 gaaccctcca ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt
     5221 aacaatccgg catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg
     5281 cgtaatgccg attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca
     5341 atttatcaat ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat
     5401 aacccggaag atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg
     5461 aactggcgtt acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttcctgc
     5521 ggtgtaatgt tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac
     5581 tgcacaatat cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat
     5641 accgtggttt tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg
     5701 aaataaacca gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt
     5761 acggcttcga caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg
     5821 gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag
     5881 ttcggtacgt acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt
     5941 tccgctactg taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa
     6001 ggacggtcga aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc
     6061 ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg
     6121 aaatgttcgc cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac
     6181 ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta
     6241 atcagcaaaa atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg
     6301 ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt aataatgcta
     6361 ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa taaaaaacct
     6421 tttcttttac gatttccgcg gcgaaaccaa aagctcatca gaatcaatgc tgcgcagcta
     6481 ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat cattgtttta
     6541 tcaggctcct ccagataatt gtcgtcatgc cggaaacccc tggcggggct attttaccgc
     6601 gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag ctaactaatc
     6661 aatgcgttga ttgtaaatcc agctaagagg tgaggttttc agagcagaca acggtgaaat
     6721 gtcatggtat tgttacgttt aggtaacaag aaatttgtct gcacaaggat tacatcatga
     6781 ttatggcgaa actgaagtca gcgaaaggga agaaatttct ctttggtttg ttggcggttt
     6841 tcattattgc ggcgtcggtt gtgactcgcg cgactatcgg cggcgttata gaacagtaca
     6901 atattccgct gtctgagtgg acgacatcaa tgtatgtgat tcagtcatcg atgatttttg
     6961 tttatagcct ggtctttact gtgttgctgg caatcccgtt gggaatttat ttccttggcg
     7021 gcgaagagca gtaagtaaaa aataggcccg ataactcggg ccttgtcagt tattgaagag
     7081 tcgttaatcg tcttcttcgt catccagttc aacgggtgtc tgatactggt caggtttaat
     7141 gaccaacaga tcgcagcgaa gatgatcaat cacctgttcc gccgtgttgc cgaggaatgc
     7201 tgctgaaata ccggtgcgtc ctaccgtgcc cagaaccaca atccccgcct gtaagtgctc
     7261 cgccaaatca ggaatcacct cttctggcag acctttttct acgtgcgtca tgttttcatt
     7321 aatgccgaat ttctgccgca gagctttcat tgccagcaaa tgttgcccac gaatggcatc
     7381 gttataaacg ctcggatcaa attccggcag ttcaatcgcg atattaattg gcgttaccgg
     7441 ataagcgcca accagatgaa cttcggtatg gttgacttgt tctgccagtt cgatcgtctc
     7501 tttgaccagt ttttcattga gcgcattatg atacggctct tcactggcga gattcaccgc
     7561 caccagcgcc ttgcctcctt ccggccacgg ctggtctttc accatccaca ccgggcttgg
     7621 gcatttgcgt aacagatgcc agtccgttgg cgtaaaaatc accgcttcca gacggtcatg
     7681 ttggtgcgcc atttttagca ccaaatcgtg cccgccgctg atcacttcct gaatgatggc
     7741 ttcgaaggga cggttatgcc agaccacttt aatttcaatg ggaacgccag cattgagata
     7801 atattttgcc tgctcgtgga tccaggctgt acgctggctg atgacgccct gacgcatagc
     7861 ggtacgttcg tccggggaga gcagggtggt catttcgtat gagaagtcat agatcggcaa
     7921 aaaggcttta attttgccac caatccgttg atgtaaataa acagctcgcc gcaatgctgg
     7981 ttggtcgtcc tggttaggat cgataacaac gagcatgttc tgatacatag ccatacaggg
     8041 tctccttaca acaactgtca acgcagtttg taattaaaag attaacccat atctggtgaa
     8101 tgaaacagtg atgaaccttc tgccagatca ataaatcaga aaaatttaat gatatgacag
     8161 aaggatagtg agttatgcgg aaaaatcagg caacgttacg cgtatgacca gcaagctggg
     8221 ccagcgcatc gttattttcg atggtgatgt atttaccttt gactgccagc ataccgcttt
     8281 tctggaagcg acccagcaga cggctgatgg tttctaccgt caggcccaga tagttaccga
     8341 tatcgccacg agtcatcgtc aggcggaatt cacgagggga gaagccgcgt tgggcaaaac
     8401 gacgggacag gttgtagatg aatgcagcca gacgttcctc ggcatttttc ttcgacaaca
     8461 gcaggatcat gtcctgatcg cctttgattt caccgctcat cagacgcatc atctgctgac
     8521 gcagattcgg cattttacca gacaaatcgt ccagcgtttc gaacgggatt tcacatacca
     8581 tcgaggtttc cagcgcctgc gcgaagctcg ggtgatggcc gctgccgatg gcgtcaaatc
     8641 ccaccaggtc gcctgctaaa tggaaaccag tgatttgctc gtcgccttgc tcagtgatgg
     8701 tataactttt aatcgtaccg gagcggatgg cataaagcga tttaagttca tcaccagcct
     8761 taaacagcgt ctggcctttc tgaataggct tcttccgctc aatgatatta tcaagctgat
     8821 caagctcatg ttcgttgagt gtgaacggga tgcaaagctg gctgatgctg caatcctggc
     8881 aatggatagc acaaccgcca gactgaatgc gccgtataat tcgcttttcc gggatcatag
     8941 gtctgctcaa gccgtaattg atatttgtca attttaacat ctttttaggg agcaagtaag
     9001 tctaagcaaa ccttaacagc agagaattcc gatattagat gtaaatatat gtctatctat
     9061 ttgaaaaccc ttaagttgtt aagggtaact ttacataaaa gtgtgaacaa gctggcacaa
     9121 attgtttaat gtttacagta aaagataacc ttcgtggcgc aataaccact cttttcgctg
     9181 aactccgcct gcatatccgg tcatggtgcc gtttcggcca ataacccgat ggcaaggtac
     9241 gacgatgctg atgggattcg atccgtttgc cgcaccaacg gcacgcgccg cgccagggcg
     9301 gcccaattgt tcagccagtt ggccgtaatg cattacctgc ccgcagggga tagtgcgtag
     9361 agttttccag acttcgcgct gaaatggcgt cccccctgtg gcagtgggaa gcgtatcaat
     9421 aatgctaaga ttaccggcaa aataatcacg aagcttgtcg cttaaaccgc ctgggttggt
     9481 ggcagaaatg cgctcatagc cttctttgcg ataatggatg tccagtagct gcaccatgcg
     9541 ttcgctgtac tcttcccatt caaccgcccg caggcgaaat tgctcatcgc aaatcaccca
     9601 cagtggaccc agtggcgtgg caattttttc ttcaagtaat ctcagcatcc gttctctctt
     9661 aagacaaacg tgggtaaatc cccggaccta tcggcagacc aacaagatac cacgccagca
     9721 acatcagcag ccataccacc aaaaagataa gcggataggg caagactaac gaatagtaag
     9781 tacccagttt cgcgtctggt ttgtagcgtt gcaggaatcc aagaaacagt ggaacaaaag
     9841 gagataccgg cgctaaaggc aatacggatg agtcggcaat acgaaagagg atttgcgcaa
     9901 atgccgggtg aaagccaagt agcataaaca ttggtacgaa aatgggggcc agaatcgacc
     9961 agattgcgga accgctggca ataaacatgc ataagaaaga ggaaagcaac gccagaccga
    10021 caaacgccgg gatgccgcta agccctgaac tttcaagtac atcggtcagc cccacggcga
    10081 tgaatttccc catgttgctc cagttaaaca tggcgacaaa ctgggcgagg ggaaaaacca
    10141 tcacgataaa tcccgccatc tctttcatcg gttcaatcat taaatgcggt aaatccgcct
    10201 gacgtcgaat tgtgcgggta gcgatgccat aagccagcga aaccacaaag aaaaaaagaa
    10261 tgattagtgg cacgatacct ttaataaagg gtgatggcat cacggtgtga ttaatcggat
    10321 cgcgcaatat cccgttttcc gggatcacca tcagcgcgat cgcagcaata aaaagtagcg
    10381 atacgacacc tgctatgcgt aaaccaaaac gctgactttc ggtcaatgtc tgcagtttct
    10441 catcgctgtt tccctgccat tgacctaacc gtggctcgat gattttgtcg gttatcaagc
    10501 cgccaacaat cgtcagtacg actacggagc tggccataaa ataccagtta tcaattacac
    10561 tgacgtgcat ttgcggattg aacgcagccg ccgcttccgt gctgatcccc gacagcaaca
    10621 cgtcggttgt gacaatcagt aaattagccg taaagccgca acctacgcct gcaatggcag
    10681 ccagtaaacc tgcaactgga tgcctgccca ccgccagaaa aatcagcgca cccatcggtg
    10741 gcatgatcac taacgccgca tcggaagaaa tgtggctgaa aaaagcaata aacagcacca
    10801 tataactggc gtagcgggca ttaacatgcg atgccatttt aaccattagc gctggcagta
    10861 agccgacgcg ctccgctagg ccggcaccta aaaccagcgc caggatcgct ccaagtggag
    10921 caaaaccgct aaagttttta atcacattgg gtaaaaacca gtgtaatcct tccacactga
    10981 gcaggttttt caccaccacc ggcgtaccat cggtcgggtt tttcgcactg acgccaaagg
    11041 ctgacaaaat tgccgtcgtc accatgagta cgattatcaa atagataaag agcaaaaaag
    11101 gatggggaac cttgttacca attctttcga cccagccata gagcttcccg gattgggagg
    11161 acgacggtat ggatgacata ctcatgggca ttcctcggtt gttgtgtttg cgttgttgtt
    11221 attttaaagg tgacggtgtc acgtttttcg ggatagggca gtgatacggt tgcgtgtccg
    11281 taacttgctg atgctcttgt tggcattctt gcaatagccc tgaatcaatg aagagattga
    11341 ctgtggttgc tgccatagtt ttcgccgcca gcagcattcc tttatgagca atagatgttc
    11401 gcccctggct aaccagttgc cacgtatgta gcggtgtacc gacggcaaaa caggggctga
    11461 aacactgggc aacaggcagt ttccaactga cgtcgccgac atcagtcgat gccgcaagca
    11521 cgttatcggt ggcggcatat ggagcgacct cattcgccag taccgtttca cgatgacgta
    11581 gtgcaaaaac cttgccgttt tcgccaccgg ttgcggcgat attattcaga ctgttttgcc
    11641 gatcgttggg ggtgagcgta gcctgaattt gtttcgcaaa agccagttct tcggagttcc
    11701 attccggggt accaaaatgg gatagggcat ggtacatggc attttctaag gtgcgattcg
    11761 ggagataact ggaacaggct ttgtcgaagc ggcattcaac cgtggtttcg gtcatcaatg
    11821 ccgcaccttc ggcgattttg gcgacccgat cataaatatg ctgcacgtcg gtcatttcgg
    11881 gggcacggat aagataaagc acttctgcct gcgcctggac cacgttgggc gagatcccgc
    11941 cgctatttgt gatggcatag tgtacgcgcg ctttttcaat aatatgttcg ttgaggaagt
    12001 tggtgccagt ggtcatcaac gttacggcat caagggcgct gcgtcccaaa tgaggggaat
    12061 tcgcggcatg tgctgcgatc cctttaaagc accatgatgc ctgaatgttt gccagcgtgc
    12121 gggtattgaa catgccggca aaggcttccg ggtgccaggt gagtgccgca tccacatcat
    12181 caaatacccc ctcgcggacc atgaacgttt tacccgagcc gccttcttcg ccaggacaac
    12241 cataaaagcg cactgtgccg ccttgcccat attgttccag ccatttcttg acggctattg
    12301 cagcggcaaa ggcggcggtt cccagcaaat tgtgtccgca accgtgacca ttttcaccgg
    12361 gcgtcgcgga tgtaggttgc gcgcaaccag cttgctgact taaacctgcc agggcgtcat
    12421 attctcccag cagggcgata accggtttgc cttgaccaaa cgaagcaata aatgcatttg
    12481 ggatattgcc tacgttgcgg gtaacggtga agcttgcaga ttccagcgcc gaagccagat
    12541 gctccgctga ccagaactct tcaaaacgtg tttctggatg atcccagatt tgatcggcaa
    12601 tatcggtata acgttggcga tcggcttcaa tcgcatcgtc gataaaacga tagatttcct
    12661 gcatcagata cctcgcgtcc agggaaaatt gagcgcggtg cgcgccagcg tttcgacggc
    12721 aatagcgaga acctgctcgt caaaatcgaa tttttcgttg tgatgacctg ccgccagctg
    12781 tgtgccaaac accacgtagg aggcttgccc ttgatgttgc tgcacgcggg ccatcattaa
    12841 tgtggcatct tcggaacccg caggcgcttc aacacgttca atggcctgat tgacccccgc
    12901 gacctgagcc gcctgacttt gcaaccatgc gacccattgc ggcgaaggag aactggcggt
    12961 agctgcaccc atcagacgag tttcaacgcc gacaccatac atggttgctg cgccctgaat
    13021 cgcttgttgt gcacggtcaa aaacatattg attaatgacg tcgctggccc cgcgtgtttc
    13081 cactttcagc aacgccgagg caggaacaac gttacggccg cttcctgcct gcataacgcc
    13141 cacgtttact ctggaagctc cttcgctgtg cggggcgatt gcatgcagtg caagagttgc
    13201 ttgtgctgcc gccaacaagg cattgtgacc gtcttctggt tttgcgcctg cgtgagcggc
    13261 ggtcccggtg aagtgcgcgt caaatttggt ggttgccata aaattatcac tgccgcacac
    13321 cacggtgccc gcaggtacgc cagtgccaat gtgcacggca gtaaaataat caacatcatc
    13381 tacgacacct gcatcgacca tcgcccgcgc gccacgcgta ccttcctctg caggctgaaa
    13441 aatcagtttg atgacgccat gtagtccgga ctcgaattgt ttaagggtat gcgccagccc
    13501 aagcccaatg gcggtatgtc catcatgacc acaggcatgc atcattccgg cgttacatga
    13561 cgcaaaaccg tcgcggtagg ggcgatggct gacatcctgc tcttcactga gatccagcgc
    13621 gtccatatcg acacggaaag ccatcaccgg accggggcga ccggtatcca gggtggcgac
    13681 gataccagtg aaaccacctt caaaaaccgc aatccattgt gctagcgcac cctgttgacg
    13741 agcgcgctcg aattcgcgtt gtagagtgag ttcatcaggt agtcccatcc ggctactttc
    13801 attaactact tcgcggccca gcgccagtga atagccgagc tgctgcaatt cttccgcaac
    13861 aagggtggca gtgcggaatt ccacccagcc agactctgca tagtgatgaa aatcacgtcg
    13921 ccagtgcgat aattttgggg caagcgaatt aacaaattga ttcaaagact ccataaccgt
    13981 tcccgtcatc agtaaaaagt gtgacccggt tcacgtagcg atagttttta cttatcacta
    14041 actgattttt cacagtttta accgttcata aattaccctg acacaatcat ctgcattaaa
    14101 gtagatgcca gtttctttgg tctgataaat aacggttatc ggtggcgtca tggcttttca
    14161 ggtaaaaatt catcaaattc gtgcttttgt tgaagtggct cgtcagggca gcattcgcgg
    14221 agcgagccga atgttgaata tgtcgcaacc ggcactgagt aaatctattc aggagctgga
    14281 agaaggatta gcggcgcaac tcttttttcg ccgtagtaaa ggcgtgacgt taactgatgc
    14341 cggtgaaagt ttttatcagc acgccagtct tattcttgaa gagctgcgcg cagcgcaaga
    14401 ggaaattcgc caacgacaag ggcaactggc agggcagatt aatatcggca tgggggccag
    14461 tatttcccgc agtctgatgc cagctgtcat atctcgtttt catcagcagc atccgcaggt
    14521 aaaagtacgc attatggaag ggcaactggt gtcgatgatt aatgaattgc gtcagggaga
    14581 attggatttc accatcaata cctattatca gggaccgtac gaccacgaat ttacttttga
    14641 gaaattactg gaaaagcaat tcgcgatctt ttgccgcccg ggacaccccg ccattggtgc
    14701 ccgttcgatc aaacagttac tggattacag ctggacaatg ccgacgccac acggcagtta
    14761 ctacaaacag ttgagtgaat tgcttgacga tcaggcgcaa acgccacagg tcggtgtagt
    14821 ctgcgagacg ttctcagcct gtatcagtct ggtggcaaaa agcgattttc tcagcatact
    14881 gcctgaagaa atgggctgcg atcccttgca cggacagggg ctggtgatgt tgccggttag
    14941 cgaaatttta ccgaaagcgg cctattattt gattcagcgg cgtgatagtc gccagacacc
    15001 actgaccgcg tcattaatca cgcaattccg gcgagaatgc ggctatctgc aaagttaaaa
    15061 ctgcataaaa aaatagagtc tgtcgacatc cgccagactc tacagtacac acagcagtgc
    15121 atccgcgtct taagtccggc attgtctcct ctgcgccggt gactgtgtta taacggtttt
    15181 aacctttagt tgccaatttt cttcagtgac agatttcacg aaaatataag ttacattatt
    15241 aatatcgtga atgaataatc atgcataagc attttgctta aaatatcggc aatatttgga
    15301 acttattact ggaattttgg gtaatacgtt gttggaccga cccggtctgg ttatcatatc
    15361 gcgctcttaa ttgcgggagg atgtaacatg aaccttgacg acaaatcgct gtttcttgac
    15421 gccatggaag atgtccagcc gctgaaacgt gctaccgatg tccactggca tccaacgcgt
    15481 aaccaacgtg cgccgcagcg tatcgacacg ctgcagcttg ataatttcct caccaccgga
    15541 tttctcgaca tcatcccact aagtcagccg ctggagtttc ggcgggaagg gttgcaacat
    15601 ggggtgctgg ataagctgcg cagtggtaaa tatccgcaac aggcgagcct gaatcttttg
    15661 cgccagccgg tggaagagtg ccgcaaaatg atgttcagtt tcattcaaca agccatggct
    15721 gatggtttgc gtaacgtgct gattattcat ggcaaaggac gggatgataa atcgcatgcc
    15781 aatattgtcc gcagctatgt ggcgcgctgg ctgaccgaat ttgatgatgt tcaggcatat
    15841 tgcactgcgc taccgcatca tggcggcagc ggggcgtgtt acgtcgcact acgtaaaacg
    15901 gcgcaggcga agcaagaaaa ctgggagcgc cacgctaagc gcagtcgttg atctcgagac
    15961 gcatccgcgg cttatgccgc cagcacgcgg ttgcgtccat catttttcgc ccgatacaaa
    16021 gcatcatcaa cgcgtttaaa cagttcatcg atgctttcat ttccttcgtg atgcgccaca
    16081 ccaatgctga cggtaaagcg tggtaagccc gaaatactca cttttgccac gcttacacgg
    16141 atagtttcag ccagcgaaag cgcggtatcc agtggggttc ttggtagcaa taagacaaac
    16201 tcttcgcctc cccaacgaaa caccaaatcg cctttgcgag cgcaactttc aagggtgcgg
    16261 gcgagggcgc ataacacctc atcaccttta gaatgcccat agagatcgtt aatgtgttta
    16321 aaacgatcgg tgtcgatgag caacaagctg taatcctgag cgatggcgag atgctgcatt
    16381 tggcctggct cggtaatgtg ataaaactgt cgccgattca gtaatccggt catcgcgtca
    16441 tggtgagcag catgttccag ctgctcctcc agccgttttt gctcagtaat atcatgcaca
    16501 atacataaca tgagcttgtc gccataaatt tcaatcggcc cggcataggt ctgcacatga
    16561 cgagtagatc catccgccag tttatgaaca aaattcaaag gtttatgacc gccgggtaaa
    16621 tgcgaaattt catgcatgat aggcatgacg cgacgcccga gcatattaat ttcccaggta
    16681 tgtttctggc acatcgtttc atggttataa ccatagaaat tgagcgcggc gaggttagca
    16741 tcgacgattt ggccatctcg tgaggggtca atcaacaaca ttggtgcaga gttagtcaga
    16801 aaaaagcgcg cataaaaacc ttgttttttg cgctgataat ttgccgagcg actggctttt
    16861 aaacccagcg ttgccggcgc ttcgatacct tcgaaaataa tcaccggttc tgtttctgtc
    16921 agctctcgca aaacaagccg acagcttaat gctgtttcct cttctttacg ctgaacagtg
    16981 aggatttcga taatatcgtg ttggttttgc agatcggaga ggtatttcgg cagttctttt
    17041 tgtgaggaga cggaataggg tccggttcgt agctgactaa acgtgaggtc ttgcatcaac
    17101 agtcgcgccg cgctattggc ataaattaac tgttcctcaa agggcgaaac aatccagaca
    17161 ggactggtga gtaagtccag ggtattgaag ttgtgcgtaa tcattgagat cccgttattt
    17221 ttatcaattt ttgttgctat ccgatcgcaa aaaagccacg tcatatgatc agataattct
    17281 gataatgata gacgctattt aacacttcac acggtttgta tacggaaaag cattttgctt
    17341 tttgtattca atttagacag aattttatta atcatttcag ggtaatgggg tgatgagatg
    17401 ttgcgtaaca gggccagaag gctagactac aaaataatgc gttgatgatg gaggcactgt
    17461 ggaagcgatt aagggatcgg acgttaatgt cccggatgca gtatttgcct ggatgctgga
    17521 tggtagaggc ggcgttaaac cgctggaaaa tacagatgtg attgatgaag cgcatccctg
    17581 ctggctccac cttaattatg tacaccatga tagcgcccaa tggctggcga caacaccgct
    17641 gcttcccaat aacgtacgtg atgcgctggc gggcgagagc acacggcccc gagtcagccg
    17701 tctcggtgaa ggcacgctta ttacattgcg ctgtataaac ggcagcaccg atgaacgccc
    17761 cgatcaactg gtcgccatgc gtgtatatat ggacgggcgg ttaattgttt cgacccgaca
    17821 acgcaaagtg ctggcgctgg acgatgtggt gagcgatctg gaagagggca cgggtccgac
    17881 cgattgcgga gggtggctgg tggatgtgtg cgatgcgttg accgatcatt ccagcgaatt
    17941 tatcgagcag ctgcacgata aaattatcga ccttgaagat aatctccttg atcagcaaat
    18001 tccaccgcgt ggattcctgg ctctgctgcg caaacaatta atcgtgatgc gtcgctatat
    18061 ggcaccgcaa cgtgatgttt atgctcgtct tgccagtgaa cgtttgccgt ggatgagcga
    18121 tgaccaacgc cgtcggatgc aggatattgc cgatcgcctt gggcgcggtc ttgacgaaat
    18181 cgacgcctgt atagcacgga ctggcgtgat ggcggatgaa atcgctcagg tgatgcagga
    18241 aaatttagct cgtcgtacct atacaatgtc attgatggca atggtctttt tacccagtac
    18301 ctttctgaca gggttatttg gcgtcaacct tggtgggatc cctggcggcg ggtggcaatt
    18361 cggattttca attttttgta ttctgttagt tgttcttatt ggtggtgttg ctttatggtt
    18421 gcaccgtagt aaatggttgt aacaaaagca atttttccgg ctgtctgtat acaaaaacgc
    18481 cgcaaagttt gagcgaagtc aataaactct ctacccattc agggcaatat ctctcttgca
    18541 ggtgaatgca acgtcaagcg atgggcgttg cgctccatat tgtcttactt ccttttttga
    18601 attactgcat agcacaattg attcgtacga cgccgacttt gatgagtcgg cttttttttg
    18661 cctgttattt atcagcgtct accctttaag agtccaccca atgaccggag ggaaatatga
    18721 cgacacttat ttatttgcaa attcctgtcc ctgaaccgat tcctggcgat cctgttccag
    18781 tgcccgatcc gatccctcgc ccgcaaccca tgcctgaccc accacctgat gaagaaccga
    18841 ttaaattgtc gcatcgtgag cgtagatctg cgaggatacg cgcctgctaa ctttgcgtcg
    18901 atgaccacga gaatagattg tgaccgcttt ttctaccctg aatgttttgc ctcccgccca
    18961 actcacgaac cttaatgagt tgggttattt aaccatgacg ccggtgcagg ccgccgcgct
    19021 tccggcgatc cttgccggaa aagatgttcg cgtgcaggcg aaaaccggca gcggcaaaac
    19081 ggcggctttt ggcctcggct tgttacagca aattgatgcg tcgctatttc aaacccaggc
    19141 tttagtgctg tgtcctacgc gtgaactggc ggatcaggtg gcaggtgaat tgcgtcggct
    19201 ggcgcgtttt ctgccaaata ccaaaatttt gacgttgtgc ggtggtcaac cgttcggtat
    19261 gcagcgtgat tcgttgcaac atgcgccgca tattatcgtg gcaacgccgg gtcgtttgct
    19321 ggatcacttg caaaaaggca cggtatcact ggatgcgctg aatactttgg tgatggatga
    19381 ggccgaccgc atgctggata tgggatttag cgatgccatt gatgatgtca tccgttttgc
    19441 gcctgcatct cgacagacgc ttctgttttc ggcaacctgg ccggaagcca tcgccgcaat
    19501 cagcggacga gtgcaacgcg atcctttggc gattgaaatt gactcaacag atgctttgcc
    19561 acccattgaa caacaatttt atgagacatc cagtaaaggc aaaattcctc tgttgcaacg
    19621 gttattaagt ttgcatcagc catcctcttg tgtggtgttt tgcaatacca aaaaagattg
    19681 ccaggctgtc tgcgacgcgc tgaatgaagt agggcaaagt gcattgtcgt tacacggcga
    19741 tctggagcaa cgcgatcgcg atcagaccct ggtacgtttt gctaacggta gcgcccgtgt
    19801 actggttgcg actgatgttg ctgcgcgtgg tctggatatt aaatcgcttg agctggtggt
    19861 gaactttgag ctggcgtggg accctgaagt tcatgtacat cgcatcggtc gtacagctcg
    19921 tgcaggaaat agcggtctgg cgatcagttt ctgtgctccg gaagaagcac agcgggccaa
    19981 tatcatttct gacatgttgc agataaaact taactggcaa acgccgccag ctaatagttc
    20041 cattttgccg ctggaagcag aaatggcaac gttgtgtatc gatggcggga aaaaagccaa
    20101 aatgcgcccg ggtgatgtat taggtgcgct gacaggagac atcgggcttg atggcgtaga
    20161 tattggcaaa atcgccgtgc atccggcgca tgtctatgtc gcggtccgtc aggctgttgc
    20221 tcataaagca tggaaacagt tacagggcgg gaagattaaa ggaaaaacgt gccgggtgcg
    20281 gttattaaaa taatgaaatg ttgaattgcc gggtgcaaga gtaaacatct tatgcgggat
    20341 tgccggatgc gacgctggcc gcgtcttatc cggcctccat aagagtagcc cgatacgctt
    20401 gcgcatcggg cgctatcctg gttatttcac ttcaaccaca ttcagccgta actcatccaa
    20461 ctgattttca tcttcttctg gctgccagcc cgccggttgt agtgggatct cttcgcgatc
    20521 aaacgccaga tcacccccgt taaccacttc agaaccgtgg gtaatgcctt tgaaatcgaa
    20581 caggttggta tcgcacagat gcgacggcac cacattctgc atcgcgctga acatcgtctc
    20641 gatacgccct ggataacgtt tatcccagtc acgcaacatg tcagcaatca cctgacgttg
    20701 caggttaggc tgtgaaccgc acaggttgca cggaataatc gggaacgctt ttgcatcggc
    20761 aaatcgctga atatctttct cgcggcagta ggccagcgga cgaataacga tatgtttgcc
    20821 atcatcgctc atcagtttcg gaggcatacc tttcatcttg ccgccgtaga acatatttaa
    20881 gaacaacgtt tgcaggatat cgtcacgatg gtgacccaac gcgatcttcg tcgcccccag
    20941 ttccgttgcg gtacgataaa ggataccgcg acgaaggcga gaacacagtg agcaagtggt
    21001 tttgccctct ggaatcttct ctttcacgat accgtaagta ttctcttcaa caatcttgta
    21061 ctcaacgccc agcttttcaa gatactcggg cagaacgtgt tccgggaagc ccggttgctt
    21121 ttgatcgagg ttaacagcca ccagcgaaaa attgattggc gcgctttgct gcaaattgcg
    21181 cagaatctcc agcatggtat agctgtcttt acccccggag aggcaaacca tgatgcgatc
    21241 gccttcttca atcatattga agtcagcaat ggcttcgccc acgttacgac gcagacgttt
    21301 ttgtaatttg ttcaggttgt attgttcttt cttactaatt tcttgatttt gcgacattta
    21361 aaagcgactc aattcgttat atggcatcag aagagtatgc gtcatgccgg aatgcccagc
    21421 ataagaaatc tgatataaaa acagtggcgt gtatggtacg gattagaggg gaaaatgtca
    21481 gcacatttgc gaaatgaatc aaaaagcccg cagcaatgtg cgggcgttag tgtcagcgca
    21541 caaccagcac ggagcactct gcgtgacgca ctacagctgc ggcgttggaa ccgagcagat
    21601 aagtggtgat atccggtcga tgggaagcaa tgatgatcat atgagcgggg atcttcttcg
    21661 ccatttccag aatgcggtct ttgggcgagc cttcctcaac atggacatgc actctgtcgg
    21721 ttggcagttt aaatttttta atgatctctt ccagttgcga tttggcttcc gctttcaggt
    21781 catccattgc cggtaattct gcggaatacg ctaaacccag agaggcatag tagggcagtg
    21841 aaggtattac cgtcaggaaa tgaacctctg catcatcaat ctttgcctct gcctcaacgt
    21901 ggctaatcac gcgttgagtt aattctgaat cggaaatatc gatagggaca agaatcgttc
    21961 tgttcataaa acctcctgtt ttagtatccg cataaagtgt aacgccagat gacacttttt
    22021 gtgtaatgac ggagttcaca tttttaattt agatcaaagg aggaagaata agcagaaaaa
    22081 gcccgccata acagcgggca ggaggattta gaactgataa accagaccta aagcgacaat
    22141 atcatcggta gagatgccat tggcagcgta gaaactgtca tcttcatcca acaggttgat
    22201 tttatagtca acataagtgg acatgttttt attgaaatag taagtcgcgc caacatcggc
    22261 gtatttaacc agatctttat catcaacacc tgccgggttg tctgcaccac cggcagcgtg
    22321 caggtcacgg cctttagaca tcaggaaaga gactgccgga cgcagaccaa aatcaaactg
    22381 gtactgtgca gtgacttcaa agttctgggt tttgtttgca acagcataat cgctgtcgcc
    22441 aaacggggtc atattacgcg tttctgaata catggttgcc aggtaaatat tgttagcatc
    22501 gtattttagc ccagcagtcc acgcgtctgc tttatcacca cccgccgcag tatggttaac
    22561 ctgatcattg gtgcggtcag aagaggtgta tgccgcacca gcgctaaagc ccatgcctaa
    22621 atcatatgtt gtggaaagac cccagccgtc accgttttca tggcgaacat cacgtccgtt
    22681 gttggtgcct tcctgaccat tactggcacc ttcgttgtta ccttgatact gcaccgcgaa
    22741 gttcagacca tttatcagac cgaagaaatc agtattacga taagtcgcga cgccattggc
    22801 tcgaccagtc ataaagttgt ctgcattggt ataagagtca ccgccaaatt caggcagcat
    22861 atcggtccag ccttcgatgt cgtacattac gccataatta cgtccgtaat cgaaagaacc
    22921 gtaatctgca aatttcagcc cggcaaatgc cagacgggtc catgactggt tttttgaaga
    22981 ttcagtgttg tttgcctgaa tattgtattc ccattgaccg tagccagtga gttgatcgtt
    23041 aatttgggtt tcgcctttaa aacccagacg cgcatagctc tggtcgccat ctttcgccga
    23101 attatcagaa aaataatgca ggccatcaac tttgccatac agatctaatt tgttgccgtc
    23161 tttattataa acttcggctg catgtgcagc acctgcggcg agcagggcag gaattaaaag
    23221 tgccagtact ttgcttttca ttgaataaat cctttagtta ttttatttgc cttttatccc
    23281 catttcgggg agtgaaaaca tcctaaagga gaagttcagt tagccgataa
//
LOCUS       DAZFVO020000077        23212 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFVO020000077 DAZFVO020000000
VERSION     DAZFVO020000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 23212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.077"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            113..1477
                     /locus_tag="ACHKNB_RS13630"
                     /old_locus_tag="pgaptmp_002726"
     CDS             113..1477
                     /locus_tag="ACHKNB_RS13630"
                     /old_locus_tag="pgaptmp_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4196212.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1605..2096)
                     /gene="yajQ"
                     /locus_tag="ACHKNB_RS13635"
                     /old_locus_tag="pgaptmp_002727"
     CDS             complement(1605..2096)
                     /gene="yajQ"
                     /locus_tag="ACHKNB_RS13635"
                     /old_locus_tag="pgaptmp_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HGU4196213.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2264..3175
                     /gene="panE"
                     /locus_tag="ACHKNB_RS13640"
                     /old_locus_tag="pgaptmp_002728"
     CDS             2264..3175
                     /gene="panE"
                     /locus_tag="ACHKNB_RS13640"
                     /old_locus_tag="pgaptmp_002728"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HGU4196214.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3138..3728
                     /gene="yajL"
                     /locus_tag="ACHKNB_RS13645"
                     /old_locus_tag="pgaptmp_002729"
     CDS             3138..3728
                     /gene="yajL"
                     /locus_tag="ACHKNB_RS13645"
                     /old_locus_tag="pgaptmp_002729"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HGU4196215.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3782..5230)
                     /gene="thiI"
                     /locus_tag="ACHKNB_RS13650"
                     /old_locus_tag="pgaptmp_002730"
     CDS             complement(3782..5230)
                     /gene="thiI"
                     /locus_tag="ACHKNB_RS13650"
                     /old_locus_tag="pgaptmp_002730"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGU4196216.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5436..5678
                     /gene="xseB"
                     /locus_tag="ACHKNB_RS13655"
                     /old_locus_tag="pgaptmp_002731"
     CDS             5436..5678
                     /gene="xseB"
                     /locus_tag="ACHKNB_RS13655"
                     /old_locus_tag="pgaptmp_002731"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGU4196217.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5678..6577
                     /gene="ispA"
                     /locus_tag="ACHKNB_RS13660"
                     /old_locus_tag="pgaptmp_002732"
     CDS             5678..6577
                     /gene="ispA"
                     /locus_tag="ACHKNB_RS13660"
                     /old_locus_tag="pgaptmp_002732"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HGU4196218.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6602..8464
                     /gene="dxs"
                     /locus_tag="ACHKNB_RS13665"
                     /old_locus_tag="pgaptmp_002733"
     CDS             6602..8464
                     /gene="dxs"
                     /locus_tag="ACHKNB_RS13665"
                     /old_locus_tag="pgaptmp_002733"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HGU4196219.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8644..9618
                     /gene="yajO"
                     /locus_tag="ACHKNB_RS13670"
                     /old_locus_tag="pgaptmp_002734"
     CDS             8644..9618
                     /gene="yajO"
                     /locus_tag="ACHKNB_RS13670"
                     /old_locus_tag="pgaptmp_002734"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HGU4196220.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9672..10190)
                     /gene="pgpA"
                     /locus_tag="ACHKNB_RS13675"
                     /old_locus_tag="pgaptmp_002735"
     CDS             complement(9672..10190)
                     /gene="pgpA"
                     /locus_tag="ACHKNB_RS13675"
                     /old_locus_tag="pgaptmp_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HGU4196221.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10168..11145)
                     /gene="thiL"
                     /locus_tag="ACHKNB_RS13680"
                     /old_locus_tag="pgaptmp_002736"
     CDS             complement(10168..11145)
                     /gene="thiL"
                     /locus_tag="ACHKNB_RS13680"
                     /old_locus_tag="pgaptmp_002736"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HGU4196222.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11223..11642)
                     /gene="nusB"
                     /locus_tag="ACHKNB_RS13685"
                     /old_locus_tag="pgaptmp_002737"
     CDS             complement(11223..11642)
                     /gene="nusB"
                     /locus_tag="ACHKNB_RS13685"
                     /old_locus_tag="pgaptmp_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGU4196223.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11662..12132)
                     /gene="ribE"
                     /locus_tag="ACHKNB_RS13690"
                     /old_locus_tag="pgaptmp_002738"
     CDS             complement(11662..12132)
                     /gene="ribE"
                     /locus_tag="ACHKNB_RS13690"
                     /old_locus_tag="pgaptmp_002738"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGU4196224.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12221..13324)
                     /gene="ribD"
                     /locus_tag="ACHKNB_RS13695"
                     /old_locus_tag="pgaptmp_002739"
     CDS             complement(12221..13324)
                     /gene="ribD"
                     /locus_tag="ACHKNB_RS13695"
                     /old_locus_tag="pgaptmp_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGU4196225.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13328..13777)
                     /gene="nrdR"
                     /locus_tag="ACHKNB_RS13700"
                     /old_locus_tag="pgaptmp_002740"
     CDS             complement(13328..13777)
                     /gene="nrdR"
                     /locus_tag="ACHKNB_RS13700"
                     /old_locus_tag="pgaptmp_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGU4196226.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13928..14467
                     /locus_tag="ACHKNB_RS13705"
                     /old_locus_tag="pgaptmp_002741"
     CDS             13928..14467
                     /locus_tag="ACHKNB_RS13705"
                     /old_locus_tag="pgaptmp_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4196227.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14766..15650
                     /gene="tsx"
                     /locus_tag="ACHKNB_RS13710"
                     /old_locus_tag="pgaptmp_002742"
     CDS             14766..15650
                     /gene="tsx"
                     /locus_tag="ACHKNB_RS13710"
                     /old_locus_tag="pgaptmp_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HGU4196228.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15827..16174)
                     /gene="yajD"
                     /locus_tag="ACHKNB_RS13715"
                     /old_locus_tag="pgaptmp_002743"
     CDS             complement(15827..16174)
                     /gene="yajD"
                     /locus_tag="ACHKNB_RS13715"
                     /old_locus_tag="pgaptmp_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HGU4196229.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16303..17274)
                     /gene="secF"
                     /locus_tag="ACHKNB_RS13720"
                     /old_locus_tag="pgaptmp_002744"
     CDS             complement(16303..17274)
                     /gene="secF"
                     /locus_tag="ACHKNB_RS13720"
                     /old_locus_tag="pgaptmp_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HGU4196230.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17285..19132)
                     /gene="secD"
                     /locus_tag="ACHKNB_RS13725"
                     /old_locus_tag="pgaptmp_002745"
     CDS             complement(17285..19132)
                     /gene="secD"
                     /locus_tag="ACHKNB_RS13725"
                     /old_locus_tag="pgaptmp_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HGU4196231.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19160..19492)
                     /gene="yajC"
                     /locus_tag="ACHKNB_RS13730"
                     /old_locus_tag="pgaptmp_002746"
     CDS             complement(19160..19492)
                     /gene="yajC"
                     /locus_tag="ACHKNB_RS13730"
                     /old_locus_tag="pgaptmp_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGU4196232.1"
                     /translation="MSFFISDAVAATGAPAQGSSMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19515..20642)
                     /gene="tgt"
                     /locus_tag="ACHKNB_RS13735"
                     /old_locus_tag="pgaptmp_002747"
     CDS             complement(19515..20642)
                     /gene="tgt"
                     /locus_tag="ACHKNB_RS13735"
                     /old_locus_tag="pgaptmp_002747"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGU4196233.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20698..21768)
                     /gene="queA"
                     /locus_tag="ACHKNB_RS13740"
                     /old_locus_tag="pgaptmp_002748"
     CDS             complement(20698..21768)
                     /gene="queA"
                     /locus_tag="ACHKNB_RS13740"
                     /old_locus_tag="pgaptmp_002748"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGU4196234.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21861..22442
                     /gene="acpH"
                     /locus_tag="ACHKNB_RS13745"
                     /old_locus_tag="pgaptmp_002749"
     CDS             21861..22442
                     /gene="acpH"
                     /locus_tag="ACHKNB_RS13745"
                     /old_locus_tag="pgaptmp_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HGU4196235.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22447..>23212)
                     /locus_tag="ACHKNB_RS13750"
                     /old_locus_tag="pgaptmp_002750"
     CDS             complement(22447..>23212)
                     /locus_tag="ACHKNB_RS13750"
                     /old_locus_tag="pgaptmp_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HGU4196236.1"
                     /translation="MQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYR
                     GFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARF
                     KTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTAL
                     FALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEVVLP
                     ASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
BASE COUNT         5617 a         6210 c         5915 g         5470 t
ORIGIN      
        1 aacatacctg acacacaaat tactctcctc tgtaatattt tctcattaac actctgttta
       61 cccaacatgc gctccgcact acactatgtg ggcatttttt taaggggtgg taatgaacga
      121 ttataaaatg acgccaggtg agaggcgcgc tacctggggt ttagggaccg tattctcgtt
      181 gcgcatgctg ggcatgttta tggttctgcc ggttctgacc acgtacggca tggctctgca
      241 aggtgccagc gaagcattaa tcggtattgc cattggtatt tatggtctga ctcaggccgt
      301 ttttcagatt ccgtttggcc tgctttccga ccgcattggt cgcaaaccat taattgtcgg
      361 tgggctggcg gtgtttgccg ccggtagcgt tatcgctgcg ctttccgact ccatctgggg
      421 aattattctg ggccgggcgc tacaaggctc tggagcaatt gccgccgccg ttatggcgct
      481 gctttccgat ctcacgcgcg aacaaaaccg caccaaagcg atggcgttta tcggcgtgag
      541 ctttggcatt acctttgcca ttgcgatggt gcttggcccg atcatcactc acaaacttgg
      601 gctgcacgcg ctgttctgga tgatcgctat tctggcaaca accggcattg cgttgaccat
      661 ttgggttgtg cccaacagta gcactcacgt acttaatcgt gagtccggaa tggtgaaagg
      721 cagtttcagt aaagtgctgg cggaaccgcg gctgctaaaa ctcaactttg gcattatgtg
      781 tctgcacatg cttctgatgt ctacgtttgt tgccctgccc ggacagctag ctgatgcggg
      841 gttcccggcg gctgaacact ggaaggtcta tctggcgacg atgttaatcg cctttggctc
      901 ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa atgaagcaag tctttgtctt
      961 ctgcgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg aacgcacaaa cgcagttctg
     1021 gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt aatttgatgg aagcccttct
     1081 gccctcactt atcagtaaag agtcgccagc aggttacaaa ggtacggcga tgggtgtcta
     1141 ctccaccagc cagtttcttg gcgtggcgat tggcggttcg ctgggcggct ggattgacgg
     1201 catgtttgac ggtcaggggg tatttctcgc tggcgcaatg ctggccgcag tgtggctggc
     1261 agtcgccagt accatgaaag aaccgccgta tgtcagcagt ttgcgcattg aaatcccggc
     1321 ggacattgcc gcaaacgaag cgttaaaagt gcgtttgctg gaaactgaag gcatcaaaga
     1381 agtgttgatt gcagaagaag aacattcagc ttatgtgaaa atcgacagca aagtgacgaa
     1441 tcgctttgag atagaacagg caattcgtca ggcataaaaa acgcggcgaa ttgcctgctg
     1501 cgctacgctt atcaggccta caaagtttct gaattacaaa cctttgtagg ccgaatcagg
     1561 cgttcatgcc gcatctgaca tgaacaaaac gcacatagtc gcgattaatc gcggaagttt
     1621 ttgaactgga acggctgacc gagatcgcca ccacgtacca tcgccatgac agcctgcaaa
     1681 tcatcacgag atttgcccgt tacgcggatt tcatcgccct gaatttgcgc ctgcactttc
     1741 agtttgctgt ctttgatcat cttgacgatt ttcttctggg tcgcgctctc aatgccctgt
     1801 ttcagtttcg cttccacaaa ccaggtttta ccgctatgaa cgatattctc cggtacatcc
     1861 agcgaactgc cttcaatgcc gcgcttcagc agcttggcac gcagaatatc cagcaactga
     1921 ttgacctgga aatcggactc gctcaacact ttgatggttt tgctggcgtc gttcagctca
     1981 aatgaggctt caacgttacg gaagtcaaaa cgggactcca cttcgcggct cgcgttatcg
     2041 accgcgttac gtgcttcctg aagatcaact tcagagacaa tatcgaaaga tggcatcttt
     2101 tcctctccct tcatttttga tgcgaagcat aatacccgca aagttaaata accgacagtc
     2161 ctgcaccagg acgtatccct taattgtgca tagtcgcaac agcatgcaca attaagggat
     2221 agccttattg aacacccgga gtggttgcgg gtgaggagga acaatgaaaa ttaccgtatt
     2281 gggatgcggt gccttagggc aattatggct tacagcactt tgcaaacagg gtcatgaagt
     2341 tcagggctgg ctgcgcgtac cgcaacctta ttgtagcgtg aatctggttg agacagatgg
     2401 ttcgatattt aacgaatcgc tgaccgccaa cgatcccgat tttcttgcca ccagcgatct
     2461 gctcctggtg acgctgaaag catggcaggt ttacgatgcc gtcaaaagcc tcgcgtccac
     2521 actgcctgta accacgccaa tactgttaat tcacaacggc atgggcacca tcgaagagtt
     2581 gcaaaacatt cagcagccat tactgatggg caccaccacc catgccgccc gccgcgacgg
     2641 caatgtcatt attcatgtgg caaacggaat cacgcatatt ggtccggcac ggcaacagga
     2701 tggcgattac agttatctgg cggatatttt gcaaaccgtg ttgcctgacg ttgcctggca
     2761 taacaatatt cgcgccgagc tgtggcgcaa gctggccgtc aactgtgtga ttaatccact
     2821 gaccgccatc tggaattgcc cgaatggtga attacgtcat catccgcaag aaattatgca
     2881 gatatgcgaa gaagtcgcgg cagtgatcga gcgcgaaggg catcatactt cagcagaaga
     2941 tttgcgtgat tacgtgatgc aggtgattga tgccacagcg gaaaatatct cgtcgatgtt
     3001 gcaggatatc cgcgcgctgc gccacaccga aatcgactat atcaatggtt ttctcttacg
     3061 ccgcgcccgc gcgcatggga ttgccgtacc ggaaaacacc cgcctgtttg aaatggtaaa
     3121 aagaaaggag agtgaatatg agcgcatcgg cactggtttg cctcgcccct ggtagtgaag
     3181 agactgaagc cgtcaccact atcgatctgc tggttcgcgg cggtatcaaa gtcaccactg
     3241 ccagcgtcgc cagcgatggc aacctggcca ttacctgctc gcgcggcgtg aagctgctgg
     3301 cggatgcgcc gctggtcgag gtggctgatg gcgaatatga cgtgatcgta ctgcctggtg
     3361 gcattaaagg cgcagagtgt tttcgcgata gtaccctgct ggttgaaacc gttaaacagt
     3421 tccaccgttc cgggcgtatc gtcgcggcta tttgcgccgc gccagccacc gtgctggtgc
     3481 cgcacgatat cttcccgatt ggtaatatga ccggcttccc gacgctgaaa gacaaaattc
     3541 ccgctgaaca atggcaggac aagcgcgtcg tctgggatgc acgggtaaaa ttgctgacca
     3601 gccaggggcc ggggacagct atcgactttg gtctgaaaat tatcgacctg ctggttgggc
     3661 gcgagaaagc ccatgaagtg gcatcacaac tggtgatggc ggcagggatt tataattatt
     3721 acgagtagtg tcggatgcgg taagcgtcgc atctgaccag atgcgacgtt acaaccacaa
     3781 attacgggcg atataccttc acattgttaa agccctgctc gcgcagatag agcgcctgca
     3841 gacggctcat caccccgcgc tcacaccaca gtagccaggt tctgttctgg tcgagatcgc
     3901 caaacttggt gctcagttta tagaacggca gagaaactac atcaatccct tcgactttca
     3961 gtggcttatc ttcctgttca tcgatagaac ggatatcgag gatcacgtcg ttcgggccga
     4021 agccattgac ggtttccact tccaccactt cctgctcggt ctgctgggcg atttcgcgga
     4081 tatcaacgtt attcgcttct tcaaccactt tatcgagaat gctgaagtcg aatttctctt
     4141 cttccgcttc aatcttcgac ttaaccgctt tcaccgtcgg gcttttggag ataacaccgc
     4201 aatattccgg catcgtgcga gcaaagtctt cggtgccaat ctggcgagcc aggttgatga
     4261 tgtgctcttt gtcgtaagaa atcagcggac gcaggatcag cgtatcggag acgttatcaa
     4321 tcaggcgcag gttggtcagc gtctgactgg acacctggcc gagcgcttcg ccggtgacca
     4381 gcgcctgtac gccgtaacgt tcagccactt tagacgcggc acgcaccatc atacgtttga
     4441 ggataacgcc catctgaccg tcgtcgattt tctcgagaat ttccccgacg accggttcga
     4501 aattaatagc gacaaaacgc acgcggtggg agctgccaaa acggttccac agataatgcg
     4561 ccacctgacg cacgccaatt tcatgcgccg cgccgccaag gttaaagaaa cagtaatgca
     4621 cgcggcagcc gcgacgcatc aacatataac tggaaacacc ggagtcgaaa ccaccggaaa
     4681 tgagcgacag cacatcttcc tgggtgccga tcgggaaacc gccaatacct tcgtagcggc
     4741 ctttaatcag caggagacga tcgtcttcca cttccagatg gacagtcact tccggattgg
     4801 tcagcttcac gcgcgcggac tcaatatgct gatttaaacc gccgccgacg taacgttcca
     4861 catcaatcga gctaaaatca tgtttgccac ggcgcttcac gcgtacgcag aaggttttgc
     4921 cttccagctg atcgcgatac tgaaccaacg ctttctcaaa aatatcgtgc atgtcggtaa
     4981 acggcacgtc ttcgacttcg agaatatggt ggatacccgg aatacgggtc agggcgtcgc
     5041 gaatagccag acgctggttt tcatcttttg cgcgaacttc gatgttatcc cagtggcgga
     5101 cgacagcgag cgtctcatca tagtgcttta aaacgttacg aatgttcccg gtaaggattt
     5161 ttataaagcg caagcgcaca gattggcttt tgatggtgat ttccgggaac aatttaatga
     5221 taaacttcat ggcggcaatg gttcgttggc aagccttaag cgacttgtat agggaaaaat
     5281 acagcagccc acacctgcgg ctgcatccag gcgcggaagt ataccactaa catcgctttg
     5341 ctgcgcacat caccttacca ttgcgcgtta tttgctattt gccctgagtc cgttaccatg
     5401 acggggcgaa aaatattgag agtcagacat tcattatgcc gaagaaaaat gaggctcccg
     5461 ccagctttga aaaggcgctg agcgagctgg aacagattgt aacccgtctg gaaagtggcg
     5521 acctgccgct ggaagaggcg ctgaacgagt tcgaacgcgg cgtgcagctg gcacgtcagg
     5581 ggcaggccaa attacaacaa gccgaacagc gcgtacaaat tctgctgtct gacaatgaag
     5641 acgcctctct aacccctttt acaccggaca atgagtaatg gactttccgc agcaactcga
     5701 agcctgcgtt aagcaggcca accaggcgct gagccgtttt atcgccccac tgccctttca
     5761 gaacactccc gtggtcgaaa ccatgcagta tggcgcatta ttaggtggta agcgcctgcg
     5821 accttttctg gtttatgcca ccggtcatat gttcggcgtt agcacaaaca cgctggacgc
     5881 acccgctgcc gccgtagagt gtatccacgc ttactcatta attcatgatg atttaccggc
     5941 gatggatgat gacgatctgc gtcgcggttt gccgacctgc catgtgaagt ttggcgaagc
     6001 aaacgcgatt ctcgctggcg acgctttaca aacgctggcg ttctcgattc taagcgatgc
     6061 tgatatgccg gaagtgtcgg atcgcgacag aatttcgatg atttctgaac tggcgagcgc
     6121 cagcggtatt gccggaatgt gcggtggtca ggcattagat ttagacgcgg aaggcaaaca
     6181 cgtacctctg gacgcgcttg agcgtattca tcgtcataaa accggcgcat tgattcgcgc
     6241 cgccgttcgc cttggtgcat taagcgccgg agataaaggg cgtcgtgctc tgccagtact
     6301 cgacaagtac gcagagagca tcggccttgc cttccaggtt caggatgaca tcctggatgt
     6361 ggtaggagat actgcaacgt tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag
     6421 tacctaccct gcacttctgg gtcttgagca agcccggaag aaagcccggg atctgatcga
     6481 cgatgcccgc cagtcgctga aacaactggc tgaacaatca ctcgatacct cggcactgga
     6541 agcgctagcg gactacatca tccagcgtaa taaataaaca ataagtatta ataggcccct
     6601 gatgagtttt gatattgcca aatacccgac cctggcactg gtcgactcca cccaggagtt
     6661 acgactgttg ccgaaagaga gtttaccgaa actctgcgac gaactgcgcc gctatttact
     6721 cgacagcgtg agccgttcca gcgggcactt cgcctccggg ctgggcacgg tcgaactgac
     6781 cgtggcgctg cactatgtct acaacacccc gtttgaccaa ttgatttggg atgtggggca
     6841 tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac aaaatcggca ccatccgtca
     6901 gaaaggcggc ctgcacccgt tcccgtggcg cggcgaaagc gaatatgacg tattaagcgt
     6961 cgggcattca tcaacctcca tcagtgccgg aattggtatt gcggttgctg ccgagaaaga
     7021 aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc gcgattaccg ctggcatggc
     7081 gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat atgctggtgg tcctcaacga
     7141 caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaat aaccatctgg cacagctgct
     7201 ttccggtaag ctttactctt cgctgcgcga aggcgggaaa aaagttttct ctggcgttcc
     7261 gccaattaaa gagctgctca aacgtaccga agaacatatt aaaggcatgg tagtgcctgg
     7321 cacgttgttt gaagagctgg gctttaacta catcggcccg gttgacggtc acgatgtgct
     7381 ggggcttatc accacgctga agaacatgcg cgacctgaaa ggcccgcagt tcctgcatat
     7441 catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa gaccccatca ctttccacgc
     7501 cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa agtagcggcg gtttgccgag
     7561 ctattcaaaa atctttggcg actggttgtg cgaaacggca gcgaaagaca acaagctgat
     7621 ggcgattact ccggcgatgc gtgaaggttc cggcatggtc gagttttcac gtaaattccc
     7681 ggatcgttac ttcgacgtgg caatcgccga gcaacacgcg gtgacctttg ccgccggtct
     7741 ggcgattggt gggtacaaac ccattgtcgc gatttactcc actttcctgc aacgcgccta
     7801 tgatcaggtg ctgcatgacg tggcgattca aaagctcccg gtcctgttcg ccatcgaccg
     7861 cgcgggcatt gttggtgctg acggtcaaac ccatcagggc gcttttgacc tctcttacct
     7921 gcgctgcata ccggaaatgg tcattatgac cccgagcgat gaaaacgaat gtcgccagat
     7981 gctctatacc ggctatcact ataacgatgg cccgtcagcg gtgcgctacc cgcgtggcaa
     8041 cgcggtcggc gtggaactga cgccgctgga aaaactacca attggcaaag gcattgtgaa
     8101 gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg ctgatgccag aagcggcgaa
     8161 agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt tttgtgaaac cgcttgatga
     8221 agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg gtcaccgtag aagaaaacgc
     8281 cattatgggc ggcgcaggca gcggcgtgaa cgaagtgcta atggcccatc gtaaaccagt
     8341 acccgtgctg aacattggcc tgcctgactt ctttattccg caaggaactc aggaagaaat
     8401 gcgcgccgaa ctcggcctcg atgccgccgg tatggaagcc aaaatcaagg cctggctggc
     8461 ataatcccta ctccactcct gctatgctta acaaattatt catagactct aaataattcg
     8521 agttgcagga aggcggcaaa cgagtgaagc cccaggagct tacataagta agtgactggg
     8581 gtgaacgaat gcagccgcag cacatgcaac ttgaagtatg acgagtatgg caggagtggc
     8641 agcatgcaat acaacccctt aggaaaaacc gaccttcgcg tttcccgact ttgcctcggc
     8701 tgtatgacct ttggcgagcc agatcgtggt aatcacgcat ggacactgcc ggaagagagt
     8761 agccgtccca tcattaaacg cgcgctggaa ggcggcataa atttctttga taccgccaat
     8821 agctattccg atggcagcag cgaagagatc gttggtcgcg cactgcggga tttcgcccgt
     8881 cgtgaagacg tggtcgttgc gacgaaagtg ttccatcgcg ttggtgattt accggaagga
     8941 ttatcccgtg cacaaatttt gcgctctatc gacgacagcc tgcgccgtct cggcatggat
     9001 tatgtcgata tcctgcaaat tcatcgctgg gattacaaca cgccgatcga agagacgctg
     9061 gaagccctga acgacgtggt aaaagccggg aaagcgcgtt atatcggcgc gtcatccatg
     9121 cacgcttcgc agtttgctca ggccctgaag ctacaaaaac agcacggctg ggcgcagttt
     9181 atcagtatgc aggatcacta caatctgatt tatcgcgaag aagagcgcga gatgctgcct
     9241 ctgtgttatc aggagggcgt ggcggtgatt ccgtggagcc cgctggcgcg ggggcgactg
     9301 acgcgtccgt ggggagaaac taccgcacga ctggtgtctg atgaggtggg gagaaatctc
     9361 tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc ggttaacggg cgtcagtgaa
     9421 gaactcggtg caacacgagc acaagttgcg ctggcctggt tgttgagtaa accgggcatt
     9481 gccgcaccga ttatcggtac atcgcgggaa gaacagcttg atgagctgct gaacgcggtg
     9541 gatatcactt tgaagccaga acagattgcc gaactggaaa cgccgtataa accgcatgcg
     9601 gtcgtaggat ttaaataaga gtccaggcct gatgagacgt gacaagcgtc acatcaggca
     9661 tcggtgcaca actacgacag aatacccagc ggccagtgat gaccgataaa gtacaggatg
     9721 cctgcggaaa tcaccccggc gacaatatcg tcgatcatga tccccatgcc gccatgtaca
     9781 ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg gaaaatcaca
     9841 aacccggcgg caacccactg ccagtcattg gtcggcagcg ccatgagcgt gatccacata
     9901 ccaataaatt cgtcccagac aatgctgccg tgatcgtgca cgcccatatc tttcgccgtt
     9961 tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga gtagagctgc
    10021 cagggtaaaa aggtcatcag ataccagaac ggaatcgctg ccagcgatcc catcgtgcca
    10081 ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca cggattactc
    10141 atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat atcctttcca
    10201 gtcaaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat cggcggtcat
    10261 ttgcccgata caggtaaacg gtacgcccag gtgtccgaga gccacatcca gcgcgccacg
    10321 gttcagttcc ggcacagtga aacacaactc gtaatcttca ccgccagaga gcgcccagcg
    10381 cagcgcctgt tccggttcaa catggcgaga aagcgcatca gaaaacggca gcaatgccag
    10441 gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg aaatcagacc
    10501 gtcagagaga tcgatagctg aatttgccag atcgcgcagt gcctgtccct gtaaaatacg
    10561 cggcgatgga cggagatgac gtttgatcaa gtagtccgca tctttagcat cggcaacctg
    10621 caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac cggtcacata
    10681 gatccagtca cccggtttcg ctccagagcg cgttaaggct cgtcccatcg gaacaaagcc
    10741 gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa tgagttgcat
    10801 atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc acgcttcgtc
    10861 tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca tcgctgccag
    10921 atcgcttagg ttcaccgcca gtgctttata cgccagatca gcaggatcga tatcatggag
    10981 gaagtggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt tctcggggat
    11041 attgagaaga gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag aacttcttac
    11101 acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt atgcctcagc
    11161 aggtaaaaaa gaaaaggccg gcgatcgcgg aacggtcttc cgtgaatcta ccggcctgga
    11221 tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc gttgacgaac
    11281 ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt aatggccact
    11341 ttgtatggca catcgctacg tttagacagc tcgtacagcg caatgcgcag tactgctttt
    11401 tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag tccgtcgagg
    11461 tatgcggtgt tagtcgccac cccggccagc agctcacgga agtacaggac gtcaacgtct
    11521 tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt ctgggacaac
    11581 tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc agcaggtttc
    11641 acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa tcatttcaag
    11701 cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac gttcgatcgc
    11761 ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc tgtcctgggc
    11821 aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaagtggg cagtgccacc
    11881 acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag ccagtgcacc
    11941 cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt tttcatcttt
    12001 tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc agcaggctgt cattgataaa
    12061 gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa cgttagcttc
    12121 aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg cggattttag
    12181 cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc actaaatgca
    12241 ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg gcgtcggcta
    12301 atttctcaag ccctggcagc gtgcataatc cgcgggcgtc gctgcctaat agtttaggtg
    12361 cgatatagac aatcagctca tcgactaaac ccacctgcag caatgcgcca gcgagcgttg
    12421 gccccgcttc cacccagatg ctgttaattt gctgtttacc cagttgcatc atcagtacaa
    12481 ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt tccggccact
    12541 cactagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc acaatgcgat
    12601 gttccggcgt cacgcgattt tggctatcaa tcacaatacg tactggctgg cggagatttt
    12661 gttgcgggta gagcgcctga gtttgttcat ccagttcaga ccaacgcact gttaaggctg
    12721 gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc atgactttgt gcgcgctgtc
    12781 gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg ctcgccatcg
    12841 ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag ccggtgcgca
    12901 tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc ccgtggctga
    12961 cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc agcgacctgc gggttaggat
    13021 cttgcatcga ggcaaccacg cgcgctacgc cagcggcgat gagtgcgtca cagcacggtg
    13081 gcgtacgacc atgatggcta cagggttcga gtgtcacata cgccgttgcg cctttggctt
    13141 tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgct cgttggtggt
    13201 aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc gggttggggt
    13261 gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa tactcgtcct
    13321 gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata tcttcgaaac
    13381 tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc aattgctcca
    13441 tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg cgcagctgcg
    13501 atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt ttttccagcg
    13561 cccgcagcat tccgctacgc aatttctctt cattaaacgg ttcacgcacg tcgttgcttt
    13621 ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt tcattacaca
    13681 ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga gagtcaatta
    13741 ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac caggtggtta
    13801 acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct tttgagataa
    13861 aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga cgagaaaggg
    13921 acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt tcagccttag
    13981 cgcctgtgtg cagcagagcg aagtgcgaca gatgaaacac agcgtcagca cgctgaacca
    14041 ggagatgacg caactcaata aagaaacggt caaaatcact cagcaaaaca ggctgaatgc
    14101 aaaatccagc agtggggttt accttctgcc aggagcgaaa acaccggcaa gactggaaag
    14161 ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatgcag atggcaccac
    14221 cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca gcggaactgt
    14281 tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg tgcagaatca
    14341 attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc cgttacagtt
    14401 aaagggtatt tctgtagaac agttagactt cgtacgtatc cacgatattc aacctgttat
    14461 gcagtaaacg tatttcgggg cgattttgtg cgtcccgcaa catctttccc cgtcattttg
    14521 ttactccgct tacatcaccc agattgatag taaaagtttg caacaagggt gaaagtcagt
    14581 acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa tgatagaact
    14641 gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc tgtatacgaa
    14701 atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca aacagtggca
    14761 tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct tcgtctttta
    14821 ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg caccagagcg
    14881 ttaacgttgt cggaagctat cacacccgtt ttggaccgca gatccgcaac gatacctacc
    14941 ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat gcggatgcgc
    15001 cggtattctt cggcggtaac tccgatgcca aaggtatctg gaaccacggc tctccgctgt
    15061 ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac cttagcttcg
    15121 gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt cgtaataaag
    15181 atggtcgcca gagcacctgg tatatgggtc tgggtaccga catcgatact ggtctgccga
    15241 tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc gcagcgaacg
    15301 aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt accgatctgt
    15361 ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc gatttagggg
    15421 atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct atcgcttcca
    15481 gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct cgttactggc
    15541 acgacggtgg tcagtggaac gacgatgcag aactgaactt cggcaacggc aacttcaacg
    15601 ttcgctctac cggctggggt ggttacctgg ttgtaggtta caacttctga ttatgaaaat
    15661 gccgggattt attcccggca tttcagattg ttaacaacgt gcgctttgtt catgccggat
    15721 gcggcgtaaa cgctttgtcc gacccacaaa agcacgtaaa ttcaatatat tgctgaaata
    15781 acgtagtcct gataagcgta gcgcatcagg caattttacg ttttaatcac ttcttcttgt
    15841 tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca tctttctgcg
    15901 catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat ttcgaatgct
    15961 catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc gggttattgg
    16021 tatggtcgtg atcaatgtgg tgaacagtaa gttcacgcag gttggaatag acaaactcgc
    16081 gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga tagccgcttt
    16141 ctaaccgcgc atagtttttt gggatgatag ccatgaaaag ttacctggat gaaatgtagg
    16201 gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc
    16261 ccgatcttct gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg
    16321 ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag
    16381 tttcagagcc aacgcagatg ccacatagat ggaagatgca gtaccgatgg aaacaccgat
    16441 aagcatggtc agcgagaagc cttccagtac cggaccaccg aagaggtaca gcatcaggat
    16501 aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac
    16561 gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac gaatacggtc
    16621 cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat
    16681 ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg
    16741 cgccagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac
    16801 gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc
    16861 cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt
    16921 aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg
    16981 catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc
    17041 ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc
    17101 gataaccgta ccaccggtaa aatccagccc ccagttaaag ccgcgcacgc ccataataac
    17161 gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa
    17221 gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca
    17281 ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac
    17341 gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga
    17401 accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga
    17461 tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa
    17521 cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga
    17581 ccgccaccgc aagggttaag acgatacccg cgatacctgg catactcagc gttgcgcctg
    17641 gcaacagcga cataatgccg acgattaaga tcaagttggc aatcagagca ctggtcgcaa
    17701 tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa
    17761 ggcaagcctc cagcccctgt tcaatgttct gcatacccag ggttgggcca atggtacgtt
    17821 cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac
    17881 gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga
    17941 tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt
    18001 tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct
    18061 tagtgaagtt agacatgatg ttaccaccag cgctatcgag cgagatgtta acctgcggtt
    18121 ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc agaattacgc
    18181 gtttgtacag cacaactggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta
    18241 cgcgaccgga tgccgcagcg gcctggtcaa cgttggtgtt taccagacgg aattccagcg
    18301 ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc ctgaatacct ggcagttcaa
    18361 caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta
    18421 cacggttacg caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac
    18481 gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg
    18541 gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtaa
    18601 tgctcaggcc gtagttgttt tctttacgaa cggtggtata cgggatgccc ttttcacgca
    18661 gatcgctgcg caggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat
    18721 ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag
    18781 cgtgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt
    18841 tgtcacccat aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca gtggagtcga
    18901 agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt
    18961 gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag
    19021 tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga
    19081 caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc
    19141 ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta
    19201 cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt
    19261 tttccgctac tttggttacg cggccaacca ggccaccgtt cgtcagaact tcatcacctt
    19321 tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga
    19381 tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgagctac
    19441 cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc
    19501 cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa
    19561 aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc
    19621 aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg
    19681 caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac
    19741 tcaggatcga gtgggccagt atcgctctta tacttcgcat tgcggatttt caccacgcca
    19801 tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata
    19861 tcgataccac gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc catcaggtaa
    19921 cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg catatctgct
    19981 ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat atctaccaga
    20041 cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat accaaacagc
    20101 gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac
    20161 atctccatgg agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag
    20221 atcatgacga tatccgaacc aagatcgtac tgaatctcca ttgatttttc aggatcgagg
    20281 aaaatcggat cgccgttgat cgggttacgg aagtgcacgc cctgttcggt gattttacga
    20341 atatcgccaa ggctgaagac ctggaaaccg ccggagtcgg tgaggatcgg ccccttccac
    20401 tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac
    20461 aggtggaagg tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc
    20521 atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca
    20581 cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc
    20641 attttttctc cactacgtca gaaaaacagt ccaacgtttt aaaccagcgc cgcggaatta
    20701 ctccccgacg cgctcattaa ttgcctgcgg attgtacgtg ataaacatcg catcaccgta
    20761 actaaaaaag cgatatttct cttctaccgc tgctttatag gcgttcatgg tgtgttgata
    20821 accggcaaag gccgaaacca gcataatcag cgtcgactct ggcaagtgga agttcgtcac
    20881 cagcgcatcg accactttgt actggaagcc cggatagata aagatttggg tatcgtcgaa
    20941 gaacggttca atgagatcgt ttttcgctgc ctgagccgcg ctttccagcg aacgtactga
    21001 agtggtgcca accgcaatca cccggttacc gcgcgctttc gccgccagta ccgcgtctac
    21061 cacatcctgc ggtacttcag cgtattccga gtgcatgatg tgatcttcaa tggtgtcgac
    21121 gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc acaaacgcca tctccacgcc
    21181 tttggcgcgc aatttttcca gcaaaggctc gtcaaaatgc agaccagcgg tcggggctgc
    21241 aaccgcgccc ggtttttcgc tataaacggt ttgataaagt tcgcggtcag cgtcttcgtc
    21301 cggacggtcg atatacggcg gcagcggcat atggccgata ctgttgagga tatccagtac
    21361 cgaacgatca tcattgaatt cgacttcaaa cagtgcgccg tggcgcgcgg tcattgttgc
    21421 gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca ggttttggcg ctttcgaggc
    21481 gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgc tcaaccagca cttcaatctt
    21541 gccgccgctg gctttacgcc caaacaggcg cgccgggatc acgcgggtat tattaaaaac
    21601 cagaagatcg ccggggttga gcttatcaag taaatcggtg aaagtaccgt gcgtcagcgc
    21661 gcccgtcggc ccgtccagcg acagtaaacg acagctactg cgttcaggca tgggatagtg
    21721 ggcaatcagg gattcgggca attcaaagga gaaatcggta acgcgcatga cactgactca
    21781 gactaaaata agaggcgggt agtctagtgc cggggcgctc tccctgcaac ctttaccccc
    21841 ttttggatag aatataatcg atgaattttt tagctcacct gcatttagcc catctcgcgg
    21901 aaagctcgct ttccggcaat ttactggctg atttcgtacg cggaaatcct gaagaaagtt
    21961 ttccgcccga cgtcgtggct ggcattcata tgcatcgacg tatcgacgta ttaaccgaca
    22021 atctgccgga agtccgcgaa gcacgggaat ggtttcgtag tgaaactcgc cgcgttgcgc
    22081 ctattacgct ggatgtcatg tgggatcact ttctttcccg ccactggtcg cagctgtcgc
    22141 cggactttcc gctacaggaa tttgtctgtt atgcccgcga gcaagtgatg acgattttgc
    22201 cggactcacc gccacgtttt atcaatctga acaattactt gtggtcagag cagtggctgg
    22261 tgcgctatcg cgatatggat ttcatccaga acgtgttaaa cggcatggca agccgccgcc
    22321 cacgtctgga tgccctgcgt gactcctggt acgatttaga cgctcattat gacgccctcg
    22381 aaacccgctt ctggcagttt tatccgcgga tgatggcgca ggcgtcacac aaggcgttat
    22441 aaaacgttag ttcatccata ccgtagccga aatggcaggc aaagccagaa tcccgtttgt
    22501 cagttgccca tggccttctt tgcgttgcca ttgcgcgaca ttaagaaacg gtgacgcggg
    22561 gagcaccact tcacaggcct cgccacggtt gattgccacc agtacacgtt gctgattcag
    22621 cacgcggaca aataccacta cgttatcttc cgcatacagc acctgacagc cgccacgacg
    22681 cagcgcctga cttttcttac gcagcgcaat cattcgctgg tacagcgcga ataacgccgt
    22741 atcctgcttt tccacctgcc aggggaacgg tttacggcaa aacggatcgt ttttgccatc
    22801 cagtcctact tcatcaccgt aataaatgca cggtacacca ggccaggtga acagccagac
    22861 caccgccagc ggcaggcgcg caatatcccg accgagcagc gttttaaatc gcgcagtatc
    22921 gtggctgtcg agctgattaa acatacgtaa ttgttgttga tgagaaagcc ctgcgcggta
    22981 attatccatc caggccatac aggtttgggc atcaatttgc tgcggatcgt aagagatatc
    23041 ggtattggca agaaatcccc acaacgggaa tgtgaagcca cgatagttca tggcggcatc
    23101 ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa tgttcgccga caatatacgc
    23161 ttccggctgg gtttctttcg ccgcttcggt gatcccagca acgtgctgca ta
//
LOCUS       DAZFVO020000078        22892 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFVO020000078 DAZFVO020000000
VERSION     DAZFVO020000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.078"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(171..2162)
                     /gene="tkt"
                     /locus_tag="ACHKNB_RS13755"
                     /old_locus_tag="pgaptmp_002751"
     CDS             complement(171..2162)
                     /gene="tkt"
                     /locus_tag="ACHKNB_RS13755"
                     /old_locus_tag="pgaptmp_002751"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGU4196237.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2440..3198
                     /gene="loiP"
                     /locus_tag="ACHKNB_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /gene_synonym="yggG"
     CDS             2440..3198
                     /gene="loiP"
                     /locus_tag="ACHKNB_RS13760"
                     /old_locus_tag="pgaptmp_002752"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HGU4196238.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3254..3997)
                     /locus_tag="ACHKNB_RS13765"
                     /old_locus_tag="pgaptmp_002753"
     CDS             complement(3254..3997)
                     /locus_tag="ACHKNB_RS13765"
                     /old_locus_tag="pgaptmp_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGU4196239.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3984..5093)
                     /gene="dgaE"
                     /locus_tag="ACHKNB_RS13770"
                     /old_locus_tag="pgaptmp_002754"
     CDS             complement(3984..5093)
                     /gene="dgaE"
                     /locus_tag="ACHKNB_RS13770"
                     /old_locus_tag="pgaptmp_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HGU4196240.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5097..5957)
                     /locus_tag="ACHKNB_RS13775"
                     /old_locus_tag="pgaptmp_002755"
     CDS             complement(5097..5957)
                     /locus_tag="ACHKNB_RS13775"
                     /old_locus_tag="pgaptmp_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGU4196241.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5954..6703)
                     /locus_tag="ACHKNB_RS13780"
                     /old_locus_tag="pgaptmp_002756"
     CDS             complement(5954..6703)
                     /locus_tag="ACHKNB_RS13780"
                     /old_locus_tag="pgaptmp_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGU4196242.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6729..7214)
                     /locus_tag="ACHKNB_RS13785"
                     /old_locus_tag="pgaptmp_002757"
     CDS             complement(6729..7214)
                     /locus_tag="ACHKNB_RS13785"
                     /old_locus_tag="pgaptmp_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HGU4196243.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7225..7653)
                     /locus_tag="ACHKNB_RS13790"
                     /old_locus_tag="pgaptmp_002758"
     CDS             complement(7225..7653)
                     /locus_tag="ACHKNB_RS13790"
                     /old_locus_tag="pgaptmp_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4196244.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7772..10570)
                     /gene="dagR"
                     /locus_tag="ACHKNB_RS13795"
                     /old_locus_tag="pgaptmp_002759"
     CDS             complement(7772..10570)
                     /gene="dagR"
                     /locus_tag="ACHKNB_RS13795"
                     /old_locus_tag="pgaptmp_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HGU4196245.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10829..11749)
                     /gene="speB"
                     /locus_tag="ACHKNB_RS13800"
                     /old_locus_tag="pgaptmp_002760"
     CDS             complement(10829..11749)
                     /gene="speB"
                     /locus_tag="ACHKNB_RS13800"
                     /old_locus_tag="pgaptmp_002760"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HGU4196246.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11885..12616)
                     /locus_tag="ACHKNB_RS13805"
                     /old_locus_tag="pgaptmp_002761"
     CDS             complement(11885..12616)
                     /locus_tag="ACHKNB_RS13805"
                     /old_locus_tag="pgaptmp_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196247.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12762..14738)
                     /gene="speA"
                     /locus_tag="ACHKNB_RS13810"
                     /old_locus_tag="pgaptmp_002762"
     CDS             complement(12762..14738)
                     /gene="speA"
                     /locus_tag="ACHKNB_RS13810"
                     /old_locus_tag="pgaptmp_002762"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HGU4196248.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14747..14878)
                     /gene="yqgB"
                     /locus_tag="ACHKNB_RS13815"
                     /old_locus_tag="pgaptmp_002763"
     CDS             complement(14747..14878)
                     /gene="yqgB"
                     /locus_tag="ACHKNB_RS13815"
                     /old_locus_tag="pgaptmp_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HGU4196249.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14972..15115)
                     /gene="yqgG"
                     /locus_tag="ACHKNB_RS13820"
                     /old_locus_tag="pgaptmp_002764"
     CDS             complement(14972..15115)
                     /gene="yqgG"
                     /locus_tag="ACHKNB_RS13820"
                     /old_locus_tag="pgaptmp_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HGU4196250.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15226..15477)
                     /gene="yqgD"
                     /locus_tag="ACHKNB_RS13825"
                     /old_locus_tag="pgaptmp_002765"
     CDS             complement(15226..15477)
                     /gene="yqgD"
                     /locus_tag="ACHKNB_RS13825"
                     /old_locus_tag="pgaptmp_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HGU4196251.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15533..16687
                     /gene="metK"
                     /locus_tag="ACHKNB_RS13830"
                     /old_locus_tag="pgaptmp_002766"
     CDS             15533..16687
                     /gene="metK"
                     /locus_tag="ACHKNB_RS13830"
                     /old_locus_tag="pgaptmp_002766"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGU4196252.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            17123..18517
                     /gene="galP"
                     /locus_tag="ACHKNB_RS13835"
                     /old_locus_tag="pgaptmp_002767"
     CDS             17123..18517
                     /gene="galP"
                     /locus_tag="ACHKNB_RS13835"
                     /old_locus_tag="pgaptmp_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HGU4196253.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            18594..19091
                     /gene="yggI"
                     /locus_tag="ACHKNB_RS13840"
                     /old_locus_tag="pgaptmp_002768"
     CDS             18594..19091
                     /gene="yggI"
                     /locus_tag="ACHKNB_RS13840"
                     /old_locus_tag="pgaptmp_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HGU4196254.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            19186..19893
                     /gene="endA"
                     /locus_tag="ACHKNB_RS13845"
                     /old_locus_tag="pgaptmp_002769"
     CDS             19186..19893
                     /gene="endA"
                     /locus_tag="ACHKNB_RS13845"
                     /old_locus_tag="pgaptmp_002769"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HGU4196255.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            19973..20704
                     /gene="rsmE"
                     /locus_tag="ACHKNB_RS13850"
                     /old_locus_tag="pgaptmp_002770"
     CDS             19973..20704
                     /gene="rsmE"
                     /locus_tag="ACHKNB_RS13850"
                     /old_locus_tag="pgaptmp_002770"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGU4196256.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            20717..21667
                     /gene="gshB"
                     /locus_tag="ACHKNB_RS13855"
                     /old_locus_tag="pgaptmp_002771"
     CDS             20717..21667
                     /gene="gshB"
                     /locus_tag="ACHKNB_RS13855"
                     /old_locus_tag="pgaptmp_002771"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HGU4196257.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            21776..22339
                     /gene="yqgE"
                     /locus_tag="ACHKNB_RS13860"
                     /old_locus_tag="pgaptmp_002772"
     CDS             21776..22339
                     /gene="yqgE"
                     /locus_tag="ACHKNB_RS13860"
                     /old_locus_tag="pgaptmp_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HGU4196258.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            22339..22755
                     /gene="ruvX"
                     /locus_tag="ACHKNB_RS13865"
                     /old_locus_tag="pgaptmp_002773"
     CDS             22339..22755
                     /gene="ruvX"
                     /locus_tag="ACHKNB_RS13865"
                     /old_locus_tag="pgaptmp_002773"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGU4196259.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
BASE COUNT         5577 a         6227 c         5805 g         5283 t
ORIGIN      
        1 tataacaaca aatgtcaata cgcatatcgt gatgcatatt acataattga tatttattgc
       61 atataaatat ttgtgatcta caacacgctt tatctattgc ttgtccgcaa acggacatat
      121 caaggtaata aaaaaggtcg ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt
      181 tcttttgctt tcgcaacaac gttatcaaca gtgaagccga actcttcaaa cagcagctct
      241 gccggagcag attcaccgaa ggtggtcata ccgacgatag caccgttcag gccaacatac
      301 ttgtaccagt agtcagcaat acccgcttct acagcaacgc gtgcagtaac cgctttcggc
      361 agtacggatt cacggtaagc agcatcctgc ttgtcaaatg cgtcggtaga cggcatggac
      421 accacgcgcg ctttcacgcc ttcggcagtc agtttttcgt aggcagcaac agccagttca
      481 acttctgaac cggtagcgat gaaaatcagt tccggctgac cggcgcagtc tttcagcaca
      541 taaccaccgc gcgcgatgtt tgccagttgc tcttcagttc gttcctgctg cgccaggttc
      601 tgacgggaga ggatcagtgc ggtcgggccg tcctgacgct caacaccgta tttccacgcg
      661 accgcggatt caacctggtc acacggacgc catgtagaca tgttcggggt tacgcgcaga
      721 gaagcgacct gctcaaccgg ctggtgagtc gggccgtctt cgcccagacc gatggagtcg
      781 tgggtgtaaa ccatcacctg acgctgtttc atcagcgcag ccatacgtac ggcgttacgt
      841 gcgtattcca cgaacatcag gaaggtggag gtgtacggca ggaaaccacc gtgcagggag
      901 ataccgttag caatcgcggt cataccgaac tcgcgaacac cgtagtggat gtagttaccc
      961 gcagcatctt cgttgattgc tttagaacca gaccacaggg tcaggttaga cggtgccagg
     1021 tcagcagaac cgccgaggaa ttccggcaac agcggaccga aagcttcgat agcattctga
     1081 gacgctttac ggctggcgat tttcgccggg ttagcctgca gtttagcaat gaactctttt
     1141 gctttcgcgt cgaagtcaga cggcatttcg cctttcatac ggcgggtaaa ttcagcggct
     1201 tcctgcggat aagctttcgc gtaagcagcg aatttctcgt tccatgcgga ttctttcgcc
     1261 tggcctgctt ctttcgcatc ccactgagca tagatttcag acgggatttc gaacggtgca
     1321 tatttccagc ccagctgttc gcgggtcagg gcaatttcag cgtcgcccag cggcgcaccg
     1381 tgggagtcgt gagtaccggc tttgttcggg gaaccgaaac cgatgatggt tttgcacatc
     1441 agcagggacg gtttgtcagt cactgcgcgc gcttcttcta ctgcgcgttt gatggatgcc
     1501 gcgtcatgac cgtcgatgtc gcgaataacg tgccagccgt aagcttcgaa acgcattgcg
     1561 gtgtcgtcgg tgaaccagcc ttcaacgtga ccatcaatag agataccgtt gtcatcgtag
     1621 aacgcaatca gtttacccag cttcagcgta cccgccagag agcaaacttc gtgggagatg
     1681 ccttccatca tgcagccgtc gcccatgaag gcataggtgt agtggtcgac aatgtcgtgg
     1741 cccggacggt taaactgcgc cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg
     1801 gcaataccct gacccagcgg accggtggtg gtttccacac cagcggtgta acccacttcc
     1861 gggtgacccg gagttttaga gtgcagctga cggaagtttt tcagttcttc catcggcaga
     1921 tcgtaaccgg tgaggtgcag caggctgtag atcagcatgg agccgtggcc gttggacagc
     1981 acgaagcggt cacggtcagc ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc
     2041 cacaggactt cggcaatgtc agccataccc ataggggcac ccgggtgacc ggatttggct
     2101 ttctgtactg cgtccatgct cagcgcacga atagcattgg caagctcttt acgtgaggac
     2161 attttgactc cagatcggat gatgaagggc acgcccttaa cgacttgacg acagcgcgtt
     2221 ttgggctacg ccggaaaatt tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga
     2281 acatcctttt gccgcttcag aaatctctgg atcatgctcg catgttgcgc aatctactcg
     2341 cccgtccgct gcgcttttcc ttatactgag actgagcgtc gattcacctg caaacggcgc
     2401 atttttagaa taatcctgac cttgtgcgga agagaaaaca tgaaaattcg cgccttattg
     2461 gtagcaatga gcgtggcaac ggtactgact ggttgccaga atatggactc caacggactg
     2521 ctctcatcag gagcggaagc ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc
     2581 ctgagcgatc aggcatgtca ggagatggac agcaaggcga cgattgcgcc agccaatagc
     2641 gaatacgcta aacgtctgac aactattgcc aatgcgctag gcaacaatat caacggtcag
     2701 ccggtgaatt acaaagtgta tatggcgaag gatgtgaacg cctttgcaat ggctaacggc
     2761 tgtatccgcg tctatagcgg gctgatggat atgatgacgg ataacgaagt cgaagcggtg
     2821 atcggtcacg agatggggca cgtggcgtta ggccatgtga agaaagggat gcaggtggcg
     2881 cttggtacaa atgccgtgcg agtagctgcg gcctctgcgg gcgggattgt cggcagttta
     2941 tctcaatcac aacttggtga tctgggcgag aaattagtca attcgcaatt ctcccagcgc
     3001 caggaagcag aagccgatga ttattcttac gatcttctgc gccaacgcgg catcagcccg
     3061 gcaggtcttg ccaccagctt tgaaaaactg gcaaaactgg aagaaggtcg ccaaagctca
     3121 atgtttgacg accatcctgc atccgccgaa cgcgcccagc atattcgcga tcgtatgagc
     3181 gcggatgggg ttaagtaagt cagacatacc gatctacaac ataattgtag gtcggtaacg
     3241 cctgaatccc cgatcagagt agtgatttca ctttatttag caaaacggct acatcatccg
     3301 gtcgggtata tcccgtatca gggtctataa tcgagctgta aacatgtggc ataatgcgcg
     3361 gcacccccgc ctccagacaa gttttcagaa tcacactgaa gttatccaaa tcaatcccgc
     3421 cagtcggctc aatcagcgtc ataccattgc gcgccgccgt ggtcgccagc gcgtatagct
     3481 ccggaagcga cctctcgccg cccatcggaa agaacttcgc cgcgtgtgcg cccatatcta
     3541 gcatcattcg caccgccgta tcgcaggaga cgcgggcagg cgtaccctgg ctgctgctaa
     3601 cgccggtgga aatcagcact tcgcccggcg tccccgtcgg gctaaccagc gcgttgacat
     3661 gggtctgctc accgcccgta gccgcaagcg cgcctgccgc gaaaccacaa ccggtaaagg
     3721 tctggttaac gtgtgctgga tgaatctgcg ccgcaatcat cgcagctttg taatactgcg
     3781 ccgggtcacc cgcccccaga cctactgaaa tggacggtat ttccgccatc caacgtttaa
     3841 cttcctggat cccctcatcc accgaggaga actgcgcgga aagcacgcca atcactgcgt
     3901 ggccttcggc ggcgtcaaag atttcacggg cattggcgat atctttcgcc agcacattaa
     3961 ttgccacgcg attacgatag aaattaatct tttgcatgat ctgcaatctc cttcagtcgc
     4021 gccacgataa gtgccatttc cccttccgcc accgtacgcg gatcaaggct aaaaacgccc
     4081 tggtggaggt tataccgccg tgcataaatc gcgatatcgc caccacgcag ttgcgcttcg
     4141 acgacgcgag cgtcaactcc caattcctgc gcatttactc gaatacgaat acgccagatt
     4201 gcacgcccgg cttcatcctg ctcaatatca gccgttaacc cgtgaatcgc agaaatcgct
     4261 tcaaccactg gctggagctg ttccgcggtg atgactgcct gcccctggtg ataattctcc
     4321 agcgcgtaaa ccagcccaac catgttctcc ttgccgattt tcatcgccct ggcaatgccg
     4381 tgatgctgcg ctttgcaggc tgcaatccag atttttttac cagtgataaa cccggaggtc
     4441 ggcgcgttaa aggcttttgc gccgctgtag accaccatat ccgctccgct caccacccag
     4501 gcacgtaaat cctcttcggc ggcggcatcg acaatcagcg gcaggttgta catctgcgcg
     4561 acatgtgcga agtcctcgat gttcaacatt cccttctgca cgcagtgatg cgacttcaca
     4621 tacagcagcg cggcggtcgc gtcggtaatg gcactctcca gctgccagag agccgccagg
     4681 ttacttgaac ccacctccac aacgcgcccg ccacctaagc ggatcgcgct gaggatcgat
     4741 gcaccgtaat cgacgttatg gccgcgcagc atcaccactt cattggccat gccggagcta
     4801 tccggcatga gcgccacccg ggccttgtcg ccgcgtgtaa tggcggcggc gacagcgatg
     4861 gcgatccctg ctgaggcaca ggaggtaacg tagctatcct ccgccccggt aaagcgagag
     4921 accagctcgc cggttttgtc caccagctgg tcaatctcca cgaaagagga tgccgcgcgc
     4981 gcggtggcct gcatcacctc cggcgcgacg ctggagacgc cgagaatcgt catcttgccg
     5041 caggcgttaa tcacctgttt cagtcccagt tgttgataga tattctgcgt catggcttac
     5101 agcactccca gcagcgagca gaccacgctc aacgccacaa tcgacagcag aatcgtggtg
     5161 taacgcggac cctttttcac caaatagaag tagatggcga acactgccgc cagcggcagc
     5221 aggccgggcg cgatggagtc cagaatctgc tgtaccacca cttcagagcc tttcagtgcg
     5281 ctaattttta aaggtgaggt tatcttgaca aagctcgcgg atagcgcgcc catcatgata
     5341 agccccagta cgttagcgcc gtagataagc tccttgatgc gccctccctg cagcagactg
     5401 ataatcgaat cgcgccccag cgtatagccc ttgtgcacca tgccgtagct gattgccagc
     5461 gtaatcgccg ggtagagaat cagcggcaaa ataccgccca tcgcgctgcc attggcggcg
     5521 aacgggataa aaatagcaat caacagcggc atcaccgccg cccagatgat ggagtcgccc
     5581 atcccggcca gcggccccat caggccggtt ttaatcccgg tgatagaggc atcgttaatc
     5641 gcctcgccgc gggtcttttg ctcttccatg gcgatggaga tcccctggat caccgcgcca
     5701 aacgtctgct ctgagttgaa gaagttcaga tggcgtttta acgcctccac ctgctcctcc
     5761 ttttgcggat agagcttttt gataatcggc gtcatcgagg cgcagaaaat caggctctgt
     5821 aaacgctcat aggagctgga aacttccgcg cccagccagt agataaacca cgctttggtg
     5881 atatccgctt tggtcagcgt cccggactcc cgcgcgcgct caaccagttc atgctgcatt
     5941 acgtcgtcgc tcattatgcc gctccttcat ttttcgccag gcctttaatc aggaaggcca
     6001 cgcaggtgcc gaaaatcgcc atcgccatga tgtccacctt gaggtacagc accgcgaaga
     6061 atccgccgat aaaccacggc agcaggctct ttttaccaat caccataatg gtgatggcga
     6121 agcccagcgc cgggagaatc ccgcccatga tttcaaacga gtgggtcagc cagtgcggca
     6181 tcagcttgag gaaactttcc actacgttct ggccgaagta gttggcggcg aacaccactg
     6241 ggaaacgtag taccagcccc agcagcgccg ggtagagaaa ggcgcagcgc ataatggctg
     6301 acatattggc cgtttccgcg tgtttatcgg ccatatgcac ccaagccgca tttagcgtgc
     6361 gacgcaactg gtcgaggaat acgccgataa cgccaaatgg aatcgccagt gcaatcgcca
     6421 ggttcgggtc catattggcc ttaacggcga taggaatggc gatacaggcc gccagcgccg
     6481 ggtctgacgg cacattgccg cctggcgttg aggtcacacc cagatagacc aactgcatcc
     6541 ccgcgccgat aatcatcgcc gcaggcatat tgccgagcag caggccgaca aagaccgcca
     6601 ccaccactgg ctgtaacagc atggcggaga aggtatagcc aaggcgcaac cgggcaaacc
     6661 agtaatacag ccccatcagg cttgcaaaaa gtaaggtatc cataatttgt tatcccatgt
     6721 tcagagggtc agaatttttt caggatatcg tccagcgact gcggtttatc ttccgggatg
     6781 gtctggaaaa cagccttaat gccgcgactt ttcaaatcgt taagaatgcc aacgtctttt
     6841 tcatccagcg ttatgttctg gaacaccgct ttacggtttg gcccgccgcc taaaccgcca
     6901 acctgaatat cagccacgtc aaagccctgc tgcaccgctt cctgaatcgc cgccagcgac
     6961 gggaacagca ccagcacgtt gccgtcgccc agctggttct ctttccacgc ggccgcgaag
     7021 ctctggttgc taaagcagtc cactttgata ttcggcggcg cggccatcag gtagatattt
     7081 ttcataaacg gatcggcgtc cagctcgtcg ctgaccaccg caatacggtt ggcctgagac
     7141 tgccctaccc acttcgtcac cacctgtccg tggataagac ggctgtcaat gcgacataac
     7201 acgatgtttg ccatacgatt ttccttactg ttgtaattga cgaatatggg agacgacgtc
     7261 caggcaactg ttgcgcccag cttccaccag atcggccacg caggcggtca gtgggccatg
     7321 ttcgcgcttg tccagcgcct ccagcagcag cgaggcatta agcccggaga tcaccgccac
     7381 cggataatcg gcgctcagcc gcgccgccac gttagaggtg gtgccgccga taaaatcggt
     7441 cacgataagc gaaccttccg gcatggtttt caccacggct tcgacgcgct ggtaaaactc
     7501 gcccagcgta tcgacgggca tcagcgcgat ttccgtcacg ccttcgattt cgcccatcac
     7561 catgcgcaga ctgttgcaga gctgcagccc ccagccgccg tgagttagca gcagaatttc
     7621 aggtaacgga tgaataggta cagttgtggt caaaatagcc tcctttgctc cagtgtttac
     7681 atttcatcag gagcaaagga cgtgccagaa tttgtgtcac gcacagagga aatgagattg
     7741 gatggggcgt taagccccgt aaaccagggg atcagctgta gagcagttcg tagatataaa
     7801 aatattctgc gtccgataaa cggatagcgt aagcctcttc aatcggcaaa aaggaggatt
     7861 tgatgacgct aaaggcacga gcgtccagat caagccggtt ttctagcgcc atctgtaacg
     7921 gtttgcggtt aatcacgata cgctcgacca tgcagcagca gtggatcaga aaacgtagtg
     7981 tcacctggcg gctcggtttc agggagagcg tgttggttaa gtagttcaga acatcctcga
     8041 tctctttcag aattcgctgc gggttgagca ccgaaatatg gttgatgatg ctctccatgg
     8101 tcagcgcgct gataaagcgc atggcgctgc gctccatctc cagtcggcgc tcgctgcttg
     8161 agagatccgg cgtcagcagg ctgagcacca gctccggccc ttgctcggaa aacagttcct
     8221 ccagcgagat aaacggtata tccggcagtc ccggctggaa ggtgccgacg atccctgcca
     8281 gccgttcact ggcattaagc gcctgctgaa tacgctccag gctgcgcacc tcgttatagt
     8341 cgagaatcac catacgcgtg tcctgcgaca tcagctcgcc aaagctctcc tccagcacct
     8401 ttttgatctt ttccgccgtc cccattccgg ttatacatga gataaccagc actttgccac
     8461 cgctctcttg ctgcggcgtg cagagccgac aagggatatt tttactctgc ataagcacag
     8521 ccaattgcga tagatcgcca gtttcgtagc ttaaatccag cccaacctcc agcaggctgg
     8581 tcagagtgat atttggcaac aacagaacgt caatctggaa cagcttgctg atggtgctgc
     8641 caaaatggac cagcgatccg atatccacca tcagtatcag ccgctgccag caccggtcct
     8701 gaatcatccg ggtcagggtt tccagggtgt catgcaccga ctgctcaaat ggcatatcaa
     8761 ttgcgtagaa cagatcgcgc tccagcaccc ggttcacata ttgcgcctgg ctcgttgcgg
     8821 tggtcgcacc gtgagcgatt agaatcacgc cgcaatccgg gctggtgtcg atgcgctggc
     8881 ggtagtggcg acactctttc aggaacaggc acagccagac cacctccgtc gccgggcact
     8941 gaatatgcaa caactcattg attttgcggc acagaagcgt ggcattgtca tactcatctt
     9001 tacagcgatc cagaatcagg ctggatgaat agagttgagg tataagccct cgccgcacat
     9061 agccaatcag cgcaagaaag tgtttacgta acgggttcgc caggttttct ggcaatgtaa
     9121 atcctaatac ctgctccacg taaccgatca gcagcgtcac acgctcctca atttgatcgc
     9181 catagcgcgg tggatgcgcc gcactatggc ggctataaag accatattca aaaatcgaac
     9241 tgagtttgtt tttcaggata gccagcgttt ctgcgggcgg cacgttactg ttgcgcagat
     9301 tgacatattc acgagtcagg aaactgtaaa acaggtcgct ctcttctatt tccgcgctgg
     9361 tcgccaacga attttttaac gcgggcaacg tacgcgcatc cacgttcaga cgatctttac
     9421 cgccaaacaa cgcatcgacc agcagacgct gctctggcgt ggtattaaac gacatttccg
     9481 ccagtcgttt atctaactct agcgtatcat tacgctcggt cattcccgac gcccacgcct
     9541 gcgcgcacaa gaactgaata tcacttttaa gctggccgat attgccttcc aggggtttac
     9601 ttaacaacca atgcaagagc gttttatcaa ggcttatggt acgttcaatt ttacgacttt
     9661 cacgctgaaa gaagcttaca atcaattcga gctgctcctc gaccgaacgc tggcgaatac
     9721 ccggtaagtc aatacatacc tggatacgcc gctggaaggt acgcaatagt gccgagttga
     9781 ccgactctgt agtggcgcaa ataaggcgca ccgaaattga atatgttggc ccacttgaac
     9841 caagtgggcg gtattcacct ttatcgagaa tagaaaagag cttctcttgt ccttcgtacg
     9901 ggaggcgatg cacttcatcc agaagtaaat aacctccgtc agcttgctcc accaatcccg
     9961 ttttattctc acttgccccg gtaaatgccc ccttatggtg accaaacaga tgtgaggaaa
    10021 gcaactccgg attatgagca tattcggcac agttaaagta aaccaacgga ggtggcgaac
    10081 ctatggtgtg cttgcaagcg aagcaatgca ttaactcagc aaagaatgtt ttgccaacac
    10141 cagatgcacc ggttaacagc acatgtagtc cgtggggata aagcaccgct gcgcggcctt
    10201 tttctaccgc atcacggaga ctacggtcgt atccgataag tccggaaaac ggatcatcat
    10261 ctgtatattt ttctttcttt ggcaataaac tagccactga atgaactatg cattcgcaat
    10321 catccagttt tcgattaatg agcaattcta gtgcgtgacg atgtagaaaa aaaacaggac
    10381 gtccctgact ttttaccacc aggccttcat tccataattg attaagatct ttactaaccg
    10441 agttgcgtgc cagcccaagg ttaaaaccaa tcgcctcagc agtaaatgct ttctccttcg
    10501 ctaaatcaga cagttccagc ccatacgtca gccgctccag ctccttgtga ataacatcaa
    10561 tccgtctcat ttctttaccg cttaaaaaac aatagttgtt cagaacatac actatttgta
    10621 aacgtaagaa tgagaactat cgggaagaat taatgttcct tgtggcaatc tggtttctga
    10681 atattcgtgt tttaaatatt acttatggtc tgaaacaacc aggcaggtga acagagctaa
    10741 gaaaaagtag cgtagaaata taaaaacata tatcgcactg atgaatgttt cccggcatga
    10801 ctatgcacac cgggaaatat ttagtaaatt actcgccctt tttcgccgcc tgaatataca
    10861 gcatctccag cgccagcgtc gccgcagcca gagcggtgat ttccgactga tcatatgccg
    10921 gagccacttc cactacgtcc atcccaacga tgttgagatc tttcaggccg cgtaccagtt
    10981 taatagcgcg atcggaggtc aggccgccaa tcactggcgt accggtgcct ggtgcaaaag
    11041 caggatccag gcagtcgata tcaaaagtca ggtaaaccgg catatcaccc acaatctgtt
    11101 tcacttgggc gataacgtca tccacgctgc gatcgttcac ctggcaggcg tccagcacgg
    11161 taaagccgtt gtctttatca aactcggtac gaataccaat ctgcacggaa tgattcgggt
    11221 cgatcagacc ttctttcggc gcggtataga acatagtgcc gtggtcaaat tcacaaccgt
    11281 tcgcataggt atcggtgtgg gcgtcaaagt gtaccagcgc cattttgccg aaatgcttcg
    11341 catgagcacg cagcagcggc agcgtaacaa agtggtcacc accgaaagag agcatacgct
    11401 taccggcagc cagcagcttc tcggcgtgcg cctgcagctt ctcgctcatc tcacgggcat
    11461 cgccgaaagc atataccaga tcaccgcagt ccacgacgtt cagacgctca cgcatgtcga
    11521 aattccacgg gaagcggttg tgttcccagg ccagattcgt cgaaacctga cggatagctg
    11581 ccggaccgtg gcgaccaccc gcacgaccag aagtggccat atcgaacggc acgccagtaa
    11641 tcacccagtc agcatcgctg tcatatggct ggaagttcat cggcaggcgt aaaaaaccaa
    11701 aggcgttgga aaccagtgag ttatcgtatt gatgacctaa ggtgctcatg gatggactcc
    11761 tattacaaag tcgatataaa aaaacccttc cgcgtcgtta ggcccgacga ggaagggttg
    11821 gatttgcgaa acacaagttt gtgctaatta tcgccttaaa tgggcgaggc atcaagcgag
    11881 gttgctattt ccgacacggt ggcggcgcat agccattgtt atctgccgga aaggcttgtc
    11941 cagagcacgg cggcagcgcg ttttgcgccg ggccttgtaa ttcaaaggcg gcaaagccga
    12001 ttaccccggt attacgcaca tcgccttgcg ccgaattggc tgcgtaggaa tcagcaacgt
    12061 tggcgaactg aaaagccgct tcagtatgtt tatctttgcg gaatccttta atcgctaacg
    12121 aatctccggc attcacaata tagccgttgt tattcagcga accttgcttg ccgttcagca
    12181 catccaggcc atcaaccgta gcaacgatct cgtagttggt gttacgactg taattacgaa
    12241 catataattg ataattcatt ccgactctgg cgtggacaat atagcgtggt tcattattgg
    12301 atgaatcata aacacgcgta ataggtagag aattaaaatt accatcgcga atagcatatt
    12361 ccagttcgtt aatacgaatg ctataaacgc ggtcgtagcc ggaaggaaca ttagtcgaat
    12421 aactaataac gatcatctcc gccggatcgc gccaggctcg ctcagcagat acgccctgaa
    12481 ccgttgagtg cacatcccca ccccaggcgg tgccgacatt acccgatgca ctgctattat
    12541 actgcgcatt actggaacaa ccggccagca gcacgattaa tgccaccaac gcgctacgta
    12601 ctttccattt tttcataacc gttccctctg catttaatta atgcagcgat tgtagggaga
    12661 ttatttaata agaaaagcag ctaaattatt tttaagaata tataaaaaga aagcggaccg
    12721 atttacgtaa ataaatcgac ccgccagcag aataatctta attattcatc ttcaagatag
    12781 gtataaccgt acaaacctgc ctcgaactct tcaaggaact gttgttgcag ttcagcatca
    12841 agatcggttt tcttcacttg atcacggaac tgagttaaca gcgttttcgg atcgagctgt
    12901 acatattgca gcatgtccgc cacggtatcg ccttcgtcag acagttccac ttcgacacta
    12961 ccgtcaggga acacgaacac gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg
    13021 ccgaggatct cctgatatgc gccgaccata aagaaaccga gcatcggcgg attctctgga
    13081 tcgtactccg gcataggcat tgtcgtggca ataccgtcac catcaatata gtggtcgata
    13141 gcaccgtcag agtcacaggt aatatccagc agcacagcgc ggcgttccgg cacttgatcc
    13201 agcccttcca gcggcagaac cgggaacaac tggtcgatcc cccacgcatc cggcatcgac
    13261 tggaacagcg agaagttgac gtacattttg tccgccatac gttcctgcag ctcgtcgata
    13321 atcggacgat gagcacggtt ttgcggatcc agctgctttt gcacttcatg gcacatgctc
    13381 aaataaagct gctcagccca tgcgcgttct tgcaggctaa aggtgccgga agagtagccg
    13441 atatgaatgt cgtgcagatc catctgactg tcgtgtaacc attcacgcag agaacggcga
    13501 gttcccggtt cgtgcatctc ctgccaggtt tcccacatgc tttgcagcgc acgcggcgca
    13561 tcttctgcag gcgcggtcgg caccgtgtat tcgttacgtt ccacgccgat gatattagac
    13621 accagcacgg tgtgatgcgc agtcactgca cgacccgatt cagtgattac cgtcggatgc
    13681 ggcagaccat tttcttcaca cgcatcgcca atcgcccaga tgatgttgtt ggcgtattca
    13741 ttgaggccat agtttacgga gcagtcagac tgcgaacggg tgccttcata gtccacgccc
    13801 agaccgccgc cgacgtcgaa gcactgaata ttgacgccca gcttgtgcag ttccacatag
    13861 aaacgcgccg attcacgaac gcctgtggcg atatcgcgaa tattcgccat ctgcgaaccg
    13921 aggtggaagt gcagtagttg caggctgtcg agacgcccgg cttcacgcag ggtttcaacc
    13981 agttgcagta cctgagtcgc agccaggccg aacttcgatt tttccccgcc ggaggactgc
    14041 catttacccg agccctgcga agccagacgt gcacgcacgc ccagacgagg aacgacattc
    14101 agacgttctg cttcatccag cacaatggcg atttctgaca tcttctcaat gaccagatag
    14161 accttgtgcc ccatcttctc gccaattaat gccaggcgga tatattcgcg gtctttataa
    14221 ccgttgcaga cgatgacgct acgggtcatg ccagcatgcg ccagcaccgc catcaactct
    14281 gctttcgagc ctgcttccag ccccaacggt tcgccagaat gaatcaggga ctcaatcacg
    14341 cggcggtgct ggttaacttt gatcggataa acaaggaagt aatcgccgtt atagccgtag
    14401 gattcccgcg cacgtttgaa cgcggcgtta atggaacgca aacggtgctg caggatctgt
    14461 gggaaacaga acagtgcagg cagacgctga ccctgtgctt cacgagtttt cactaactgc
    14521 gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc acacgctgat gtggcccagc
    14581 tcgttaacgt catagtagtt attgccccac caggcaatat tgtaagtacg cagcatcttg
    14641 ctggcttcct gggagctcat tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc
    14701 gctgacgaag gcaaacccat agacatgtcg tcagacatag cgaacctcaa attattttat
    14761 taagtgtaaa acagttaacg actatcgcag cctggaactg cgataacaac ccataagcac
    14821 atggattttc cagcagtgaa tgctgacgct ccaactgcgc aaccggtttc tttttcataa
    14881 cattattaag cacataaccg aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc
    14941 ttgtttttac aagagcgccc ttgttcagtc ctcagtaact gtaaccagct cttgaatcct
    15001 gagaagcgcc gagatgggta taacatcggc aggtatgcaa agcagagatg cagagtgcgg
    15061 ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag cagacaacgg ttcattattt
    15121 cgtatcacct ccacggccgc ctgttaagac gaacccacaa gccaaaactc tgatttcaac
    15181 ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg
    15241 cgttttatac cccacaatgg cagaaaattg caaaagataa atacgcagaa tgccggcatt
    15301 gtcaggaaaa atttccagcc acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg
    15361 aaatcgggtg aagaattgac ctaaaatagc catccagatg ttaatccatc cataccgatt
    15421 aacactcaga ctgccagtgt ttttaacttg cagagtcgtg gtaggatccg ctaccacaga
    15481 aaatccacac aacagtttga gctaaccaaa ttctctttag gtgatattaa atatggcaaa
    15541 acaccttttt acgtccgagt ccgtctctga agggcatcct gacaaaattg ctgaccaaat
    15601 ctctgatgcc gttttagacg cgatcctcga acaggatcca aaagcacgcg ttgcttgcga
    15661 aacctacgta aaaaccggca tggttttagt tggcggcgaa atcaccacca gcgcctgggt
    15721 agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt ggctatgtgc attccgacat
    15781 gggctttgac gctaactcct gtgcggttct gagcgctatc ggcaaacagt ctcctgacat
    15841 caaccagggc gttgaccgtg ccgatccgct ggaacagggc gcgggtgacc agggtctgat
    15901 gtttggctac gcaactaatg aaaccgacgt gctgatgcca gcacctatca cctatgcaca
    15961 ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc
    16021 ggacgcgaaa agccaggtga ctttccagta tgacgacggc aaaatcgttg gtatcgatgc
    16081 tgtcgtgctt tccactcagc actctgaaga gatcgaccag aaatcgctgc aagaagcggt
    16141 aatggaagag atcatcaagc cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt
    16201 cttcatcaac ccgaccggtc gttttgttat cggtggcccg atgggtgact gcggtctgac
    16261 tggtcgtaaa attatcgttg atacctacgg cggcatggca cgtcacggtg gcggagcatt
    16321 ctctggtaaa gatccatcaa aagtggaccg ttccgctgcg tacgcagcac gttatgttgc
    16381 aaaaaacatc gttgctgctg gcctggccga tcgttgtgaa attcaggttt cctacgcaat
    16441 cggcgtggct gaaccgacct ccatcatggt agaaactttc ggtactgaga aagtgccttc
    16501 tgaacaactg actctgctgg tacgtgagtt cttcgacctg cgcccatacg gtctgattca
    16561 gatgctggat ctgctgcacc cgatctacaa agaaaccgca gcatacggtc actttggtcg
    16621 tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg ctgcgcgatg ctgccggtct
    16681 gaagtaatct ttcttcacct gctttcaaag gccagcctcg cgctggcctt tttcttttac
    16741 ctcaccgcgg tggataggca ttcacgccgc atccggcaaa aagctgcccg cacaataaca
    16801 tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac tactgaaacc gattacacca
    16861 ataacaccaa ccaaatattt gtaatatttt catattatta ttcagttttc acagttgtta
    16921 catttctttt cagtaaagtc ttaattgcag ataacagcgt ttaatctatg atgatataac
    16981 tcaattattt tcatgcactt aaaccataac taagataaat gttagtgtaa gcgattacac
    17041 tgatgtgatt tgcttcacat ctttttacgt cgtactcacc tatcttaatt cacaataaaa
    17101 aataaccata ttggagggca tcatgcctga cgctaaaaaa caggggcggt caaacaaggc
    17161 aatgacgttt ttcgtctgct tccttgccgc tctggcggga ttactctttg gcctggatat
    17221 cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa ttccagatta cttcgcacac
    17281 gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca gtcggtgcgg tgggcagcgg
    17341 ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg atcggcgcaa ttttgtttgt
    17401 tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa gtactgattc tttcccgcgt
    17461 tctactgggg ctggcggtgg gtgtggcctc ttataccgca ccgctgtacc tctctgaaat
    17521 tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat cagttgatga tcactatcgg
    17581 gatcctcggt gcttatcttt ctgataccgc cttcagctac accggtgcat ggcgctggat
    17641 gctgggtgtg attatcatcc cggcaatttt gctgctgatt ggtgtcttct tcctgccaga
    17701 cagcccacgt tggtttgccg ccaaacgccg ttttgttgat gccgaacgcg tgctgctacg
    17761 cctgcgtgac accagcgcgg aagcgaaacg cgaactggat gaaatccgtg aaagtttgca
    17821 ggttaaacag agtggctggg cgctgtttaa agagaacagc aacttccgcc gcgcggtgtt
    17881 ccttggcgta ctgttgcagg taatgcagca attcaccggg atgaacgtca tcatgtatta
    17941 cgcgccgaaa atcttcgaac tggcgggtta taccaacact accgagcaaa tgtgggggac
    18001 cgtgattgtc ggcctgacca acgtacttgc cacctttatc gcaatcggcc ttgttgaccg
    18061 ctggggacgt aaaccaacgc taacgctggg cttcctggtg atggctgctg gcatgggcgt
    18121 actcggtaca atgatgcata taggtattca ctctccgtca gcgcagtatt tcgccatcgc
    18181 catgctgctg atgtttattg tcggttttgc catgagtgcc ggtccgctga tttgggtact
    18241 gtgctccgaa attcagccgc tgaaaggccg cgattttggc atcacctgct ccaccgccac
    18301 caactggatt gccaacatga tcgttggcgc aacgttcctg accatgctca acacgctggg
    18361 taacgccaac accttctggg tgtatgcggc tctgaacgta ctgtttatcc tgctgacatt
    18421 gtggctagta ccggaaacca aacacgtttc gctggaacat attgaacgta atctgatgaa
    18481 aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct cctcaggctt cctcccgtcg
    18541 cggaggaagc cacctcttgc agtcatcttt tcttcgttct atcctctgcc gctatgaaaa
    18601 catcccgtct ccctatcgcc atccaacagg ccgttatgcg ccgcctgcgg gaaaaactcg
    18661 cccaggccaa cctgaagcta gggcgtaact acccggagcc aaaactctct tacacccagc
    18721 gcggaacctc cgccggaacg gcctggctgg aaagctatga aattcgcctc aatcccgttt
    18781 tgctgttgga aaacagtgaa gcttttattg aagaagtggt accgcacgaa ctggcacatt
    18841 tgctggtatg gaaacatttc ggccgcgtag cgccacatgg caaagagtgg aagtggatga
    18901 tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca gttcgaactg caatccgtgc
    18961 gtcgcaacac cttcccctac cgctgcaagt gccaggagca tcagcttacc gtacgccgcc
    19021 ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt tcactgcggt gaacagctgg
    19081 ttgcgaaata accatctgaa ctatcaggaa ctttcctgat ctggctgatt gcataccaaa
    19141 acaactttcg ctacgttgct ggctcgtttt aacacggagt aagtgatgta ccgttatttg
    19201 tctattgctg cggtggtact gagcgcagca ttttccggcc cggcgttggc cgaaggtatc
    19261 aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc acgctgacgc gcccggtacg
    19321 ttttattgcg gatgtaaaat taactggcag ggcaaaaaag gcgttgttga tctgcaatcg
    19381 tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc gcgtagagtg ggaacacgtc
    19441 gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc aggacggtgg acgtaaaaac
    19501 tgcgctaaag atccggtcta tcgcaagatg gaaagcgata tgcataacct gcagccgtca
    19561 gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca gccagtggaa tggcggcgaa
    19621 ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag aaaaagctgc cgaaccacca
    19681 gcgcgtgcac gcggtgccat tgcacgcacc tacttctata tgcgcgacca atacaacctg
    19741 acactctctc gccagcaaac gcagctgttc aacgcatgga acaagatgta tccggttacc
    19801 gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc aaggcaatca taatccgtat
    19861 gtgcaacgcg cttgccaggc gcgaaagagc taacctacac tagcgggact ctttttgtta
    19921 acccctaccc cacgcgtaca accgcgtggg gagacgacgc ggatttttaa ctatgcgtat
    19981 cccccgcatt tatcatcctg aaccactgac cagccattct cacatcgcgc tttgcgaaga
    20041 tgccgccaac catatcgggc gcgtactgcg catggggccg gggcaggcgt tgcaattgtt
    20101 tgacggtagc aaccaggtct ttgacgccga aattaccagc gccagcaaaa aaagcgtgga
    20161 agtgaaggtg ctggaaggca agatcgacga tcgcgaatct ccgctgcata ttcacctcgg
    20221 tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc cagaaatcga tcgaactcgg
    20281 tgtaagcctc attacgccac ttttttctga gcgctgcggc gttaaactgg atagtgaacg
    20341 tctgaacaag aagctccagc agtggcagaa gattgcaatt gctgcctgtg agcagtgtgg
    20401 tcgtaaccgg gtgccggaaa tccgcccagc gatggatctg gaagcctggt gtgcagagca
    20461 ggatgaagga ctgaaactga atcttcaccc gcgcgccagt aacagcatca atacgttgcc
    20521 gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa ggcggtttat cggcagatga
    20581 aattgccatg actgcccgct atcaatttac tgatatcctg ttgggacctc gcgttttgcg
    20641 tacagagaca actgcgctca ccgccattac cgcgctacaa gtacgatttg gcgatttggg
    20701 ctaacggaga agaataatga tcaagctcgg catcgtgatg gaccccatcg caaacatcaa
    20761 catcaagaaa gattccagtt tcgctatgtt gctggaagca cagcgtcgtg gttacgaact
    20821 tcactatatg gagatgggcg atctgtatct gatcaatggc gaagcccgcg cccatacccg
    20881 cacgctgaac gtgaagcaga actacgaaga gtggttttca ttcgtcggtg aacaggatct
    20941 gccgctggcc gatctcgatg tgatcctgat gcgtaaagac ccgccgtttg ataccgagtt
    21001 tatctacgcg acctatattc tggaacgtgc cgaagagaaa gggacgctga tcgttaacaa
    21061 gccgcagagc ctgcgcgact gtaacgagaa actgtttacc gcctggttct ctgacttaac
    21121 gccagaaacg ctggttacgc gcaataaagc gcagctaaaa gcgttctggg agaaacacag
    21181 cgacatcatt cttaagccgc tggacggtat gggcggcgcg tcgattttcc gcgtgaaaga
    21241 aggcgatcca aacctcggcg tgattgccga aaccctgact gagcatggca ctcgctactg
    21301 catggcgcaa aattacctgc cagccattaa agatggcgac aaacgcgtgc tggtggtgga
    21361 tggcgagccg gtaccgtact gcctggcgcg tattccgcag gggggcgaaa cccgtggcaa
    21421 tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg gaaagtgact ggaaaatcgc
    21481 ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt tttgttggtc tggatatcat
    21541 cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg agattgaagc
    21601 agagtttccg gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac gtttacagca
    21661 gcagtaaccc accttagcga gaaggatctc gttgagactc tgagtgacag cgcccttctt
    21721 tccacgcata ctgggcgctg ttgctttttt gaaccaggaa acagaacctc tgacaatgaa
    21781 tttacagcat cactttctta ttgccatgcc tgctctccag gatccgattt tccgtcgttc
    21841 cgtggtctac atttgcgaac ataataccaa tggtgcaatg gggatcatcg tcaacaagcc
    21901 gctggaaaat ctcaaaattg aagggattct ggaaaagctg aagatcacgc cggagccgcg
    21961 tgatgaatca atccgtctgg ataaaccggt tatgctcggc ggtccgctgg ctgaagatcg
    22021 cgggtttatt ttgcatactc cgccctccaa ttttgcttcc agcattcgca tttcagacaa
    22081 cacagtgatg accacttccc gcgatgtgct ggaaacgctc ggcaccgata aacaaccgtc
    22141 tgacgtattg gtggctctgg gttatgcctc ctgggaaaaa ggtcaactgg aacaagaaat
    22201 tctcgataac gcgtggctaa cggccccggc ggatctgaat attctgttca aaacgccgat
    22261 tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg gatattctca ccatgcctgg
    22321 tgtggcaggg cacgcctgat gagtggaacc ttactcgcct tcgacttcgg caccaaaagc
    22381 attggcgtag cggtcggcca acgcattacc ggcactgctc gccctttgcc tgcaattaaa
    22441 gcacaggacg gtacgccgga ctggaacctc atcgaacgtt tactgaaaga gtggcagccg
    22501 gacgaaatca tcgtcggttt gccgctgaat atggacggca ccgagcaacc attgactgcc
    22561 agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt aaagctccat
    22621 gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt ttgaacaggg cggctatcgg
    22681 gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta ttattctcga aagctatttc
    22741 gagcagggat attaaggcga tctaaaagcc tggcggatag tcgttaccgc catcttgccg
    22801 gatgcggcgt gaacgcctta tccagtacac atctggcagc ggctgtaggt ctgataagac
    22861 gcgttagcgt cgcatcagac atttattgcc tt
//
LOCUS       DAZFVO020000079        22334 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFVO020000079 DAZFVO020000000
VERSION     DAZFVO020000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.079"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            188..871
                     /locus_tag="ACHKNB_RS13870"
                     /old_locus_tag="pgaptmp_002774"
     CDS             188..871
                     /locus_tag="ACHKNB_RS13870"
                     /old_locus_tag="pgaptmp_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HGU4196260.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            915..5765
                     /gene="ehaG"
                     /locus_tag="ACHKNB_RS13875"
                     /old_locus_tag="pgaptmp_002775"
     CDS             915..5765
                     /gene="ehaG"
                     /locus_tag="ACHKNB_RS13875"
                     /old_locus_tag="pgaptmp_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HGU4196261.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVSNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6133..7788
                     /gene="lldP"
                     /locus_tag="ACHKNB_RS13880"
                     /old_locus_tag="pgaptmp_002776"
     CDS             6133..7788
                     /gene="lldP"
                     /locus_tag="ACHKNB_RS13880"
                     /old_locus_tag="pgaptmp_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HGU4196262.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7788..8564
                     /gene="lldR"
                     /locus_tag="ACHKNB_RS13885"
                     /old_locus_tag="pgaptmp_002777"
     CDS             7788..8564
                     /gene="lldR"
                     /locus_tag="ACHKNB_RS13885"
                     /old_locus_tag="pgaptmp_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HGU4196263.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8561..9751
                     /gene="lldD"
                     /locus_tag="ACHKNB_RS13890"
                     /old_locus_tag="pgaptmp_002778"
     CDS             8561..9751
                     /gene="lldD"
                     /locus_tag="ACHKNB_RS13890"
                     /old_locus_tag="pgaptmp_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HGU4196264.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKEFPAALAPMAK
                     GNAA"
     gene            9906..10379
                     /gene="trmL"
                     /locus_tag="ACHKNB_RS13895"
                     /old_locus_tag="pgaptmp_002779"
     CDS             9906..10379
                     /gene="trmL"
                     /locus_tag="ACHKNB_RS13895"
                     /old_locus_tag="pgaptmp_002779"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HGU4196265.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10432..11253)
                     /gene="cysE"
                     /locus_tag="ACHKNB_RS13900"
                     /old_locus_tag="pgaptmp_002780"
     CDS             complement(10432..11253)
                     /gene="cysE"
                     /locus_tag="ACHKNB_RS13900"
                     /old_locus_tag="pgaptmp_002780"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGU4196266.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11333..12352)
                     /gene="gpsA"
                     /locus_tag="ACHKNB_RS13905"
                     /old_locus_tag="pgaptmp_002781"
     CDS             complement(11333..12352)
                     /gene="gpsA"
                     /locus_tag="ACHKNB_RS13905"
                     /old_locus_tag="pgaptmp_002781"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGU4196267.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPPMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12352..12819)
                     /gene="secB"
                     /locus_tag="ACHKNB_RS13910"
                     /old_locus_tag="pgaptmp_002782"
     CDS             complement(12352..12819)
                     /gene="secB"
                     /locus_tag="ACHKNB_RS13910"
                     /old_locus_tag="pgaptmp_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGU4196268.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12882..13133)
                     /gene="grxC"
                     /locus_tag="ACHKNB_RS13915"
                     /old_locus_tag="pgaptmp_002783"
     CDS             complement(12882..13133)
                     /gene="grxC"
                     /locus_tag="ACHKNB_RS13915"
                     /old_locus_tag="pgaptmp_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HGU4196269.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13275..13706)
                     /locus_tag="ACHKNB_RS13920"
                     /old_locus_tag="pgaptmp_002784"
     CDS             complement(13275..13706)
                     /locus_tag="ACHKNB_RS13920"
                     /old_locus_tag="pgaptmp_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGU4196270.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13951..15495
                     /gene="gpmM"
                     /locus_tag="ACHKNB_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /gene_synonym="pgmI"
     CDS             13951..15495
                     /gene="gpmM"
                     /locus_tag="ACHKNB_RS13925"
                     /old_locus_tag="pgaptmp_002785"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HGU4196271.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15529..16788
                     /gene="envC"
                     /locus_tag="ACHKNB_RS13930"
                     /old_locus_tag="pgaptmp_002786"
     CDS             15529..16788
                     /gene="envC"
                     /locus_tag="ACHKNB_RS13930"
                     /old_locus_tag="pgaptmp_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HGU4196272.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16792..17751
                     /locus_tag="ACHKNB_RS13935"
                     /old_locus_tag="pgaptmp_002787"
     CDS             16792..17751
                     /locus_tag="ACHKNB_RS13935"
                     /old_locus_tag="pgaptmp_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HGU4196273.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17738..18772)
                     /gene="waaH"
                     /locus_tag="ACHKNB_RS13940"
                     /old_locus_tag="pgaptmp_002788"
     CDS             complement(17738..18772)
                     /gene="waaH"
                     /locus_tag="ACHKNB_RS13940"
                     /old_locus_tag="pgaptmp_002788"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HGU4196274.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19011..20036)
                     /gene="tdh"
                     /locus_tag="ACHKNB_RS13945"
                     /old_locus_tag="pgaptmp_002789"
     CDS             complement(19011..20036)
                     /gene="tdh"
                     /locus_tag="ACHKNB_RS13945"
                     /old_locus_tag="pgaptmp_002789"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HGU4196275.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20046..21242)
                     /gene="kbl"
                     /locus_tag="ACHKNB_RS13950"
                     /old_locus_tag="pgaptmp_002790"
     CDS             complement(20046..21242)
                     /gene="kbl"
                     /locus_tag="ACHKNB_RS13950"
                     /old_locus_tag="pgaptmp_002790"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HGU4196276.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPYLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21517..>22334)
                     /gene="yibB"
                     /locus_tag="ACHKNB_RS13955"
                     /old_locus_tag="pgaptmp_002791"
     CDS             complement(21517..>22334)
                     /gene="yibB"
                     /locus_tag="ACHKNB_RS13955"
                     /old_locus_tag="pgaptmp_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HGU4196277.1"
                     /translation="GRGDWTANKGFREKLARSVDVYFSYFERLAALENEMIIFTSPDL
                     KPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQLKNPEYWSPE
                     YVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHLF
                     TIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVD
                     DDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5693 a         5747 c         5663 g         5231 t
ORIGIN      
        1 aaataatata ttcatacaaa cacagacatc ttttgaaacg aatgatacat ttttatgatg
       61 aatgcttaaa acaatattta atcgacacac atcactgtca ttaatgtaaa gcaacataga
      121 tcaccaataa tacaatggac aactctaccg gcattgatcg ctggtcggag gaaataagga
      181 tttttacatg aacttgaaga agattttttt cagtgctgtc actgtttcag ttctctgcgc
      241 attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttat tgaaacaaca
      301 agaagaacat aacaataaaa ttgatctact tgaaaaacaa caagcacagc taaagagtca
      361 actggaaact attcaaaaac aacaaactgg cataataagc agtacaaaga ctttaactca
      421 cgtaattaaa tccgttaaag atcaacaaaa cacttttatt tttaccgagt ttaacccagc
      481 aaaaacgaag tatttcatcc tgaacaatgg ttcggtggct ttagcgggtc gagtgttatc
      541 cattgacgcc acagagaatg gcagtgttat tcatatttca ctggtcaact tattaagtac
      601 acctatctca aatattggtt ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa
      661 agagtttgcc cggtggcaac aattactttt caacacatca atgaaatcca cattgaaatt
      721 attaccaggt caatggcaag acattaattt gaccctgaag ggtgtatcgc ccaataacct
      781 gggatatctg aagttagcca tcaacatgga aaatattcag ttcgacaatc ttccctctgc
      841 tgaaaatcgg cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat aacaaggaat
      901 agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt
      961 atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt
     1021 taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg
     1081 ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg
     1141 ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg
     1201 tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg
     1261 cgattggcgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta
     1321 tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt
     1381 caaaagcatt gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa
     1441 acgccattgc cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg
     1501 acaccgccaa tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg
     1561 aagaaaacgc aattgccctg gggcgtagca gtgtagctag cggtactgac agcctcgcat
     1621 ttggcagaca atcacttgcc agcgcagcga acgctattgc gataggtgct gagaccgaag
     1681 ccgctgaaaa tgcaactgct attggcaata atgcgaaggc aaaagggact aatagcatgg
     1741 caatggggtt cggaagcctt gccgataaag tcaatactat cgcattagga aatggcagcc
     1801 aggctctggc agataatgca atcgccatag gccagggcaa caaagctgat ggcgtggatg
     1861 ccatcgctct gggtaatggt agccagtcga gaggcttaaa caccattgcc ttaggcacag
     1921 ccagtaatgc aactggtgat aagagtcttg cgcttggtag taatagcagt gccaacggta
     1981 ttaactctgt cgcgctgggc gcagattcca ttgcggattt agacaatacc gtctctgtca
     2041 gcaatagttc attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca
     2101 gttacgatgc cattaatggt tcacagcttt atgccattag cgactcggta gcaaaaaggc
     2161 ttggaggagg ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt
     2221 taaaaaatgg tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc
     2281 tgcaatggga ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg
     2341 ccagcgtaat caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta
     2401 acggttccca actgaaagct accaatgacg atgtcgaagc caacaccgcc aatatcgcta
     2461 ctaataccag caacattgcc acgaatacgg caaatattgc caccaatacc accaatatca
     2521 ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg aacgaaacta
     2581 aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc aacgttaaag
     2641 atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg aaaaccacca
     2701 acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat attgccacta
     2761 acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     2821 aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc accagcaaaa
     2881 tcaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt aacggctctc
     2941 agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc actaacacca
     3001 ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg
     3061 ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc aaaatcacca
     3121 atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt agccagcttt
     3181 atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg aatactgacg
     3241 gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg
     3301 cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg ctttgggatg
     3361 ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc gtgatcactg
     3421 atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc tcacaactcc
     3481 acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg
     3541 gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat gtcggtgatg
     3601 ccctgaatgc tatcgatacc actcttgacg acgctctgct ctgggatgcg gacgccggtg
     3661 aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta atcactaacg
     3721 tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca caactctata
     3781 ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac gctgacggca
     3841 ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc ggtgacgccc
     3901 tggatgcgct tgatgataac gccctgctgt gggatgagac tgccaatggc ggtgctggag
     3961 cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct aatggcagta
     4021 ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg aatatgatga
     4081 ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca acctatatcc
     4141 aagaaaacgg tgcgggtatt aactatgtgc gtactaacga cgacggctta gcgttcaacg
     4201 acgccagcgc acagggtgtt ggcgctacag ctataggtta taactctgtc gccaaaggcg
     4261 atagcagcgt agctattggt cagggcagct acagcgacgt tgatacgggt atcgccctgg
     4321 gtagcagctc tgtttccagc cgagtgattg ccaaaggctc ccgtgacacc agcataacgg
     4381 aaaatggcgt tgttattggt tacgacacca cggatggcga actgctcggt gcattgtcta
     4441 tcggtgatga cggtaaatat cgtcaaatca tcaacgtagc cgatggttcc gaagcccatg
     4501 acgccgttac ggttcgtcaa ttgcagaatg cgattggtgc ggtcgcaacc acgccgacta
     4561 aatacttcca cgctaattca acggaagaag attcactggc agtgggaact gactcgctgg
     4621 caatgggtgc gaaaaccatc gtgaatggcg ataaaggtat tggtatcggt tatggtgcct
     4681 acgtggacgc gaatgcactt aacggcattg ccattggtag caatgcgcaa gtcattcatg
     4741 tcaacagtat tgcgataggt aatggttcta cgaccactcg tggcgctcaa accaattata
     4801 ccgcctacaa catggacgca ccgcagaact ctgtcggtga attctcagtc ggtagtgcgg
     4861 atggtcaacg tcagatcact aacgtcgcag caggttcggc tgataccgat gcggtcaacg
     4921 tgggtcagtt gaaagtaacg gatgcgcagg tttcccagaa tacccagagc attactaacc
     4981 tggataatcg ggtaacgaat cttgattcac gcgtcaccaa tatcgaaaac ggtattggcg
     5041 atatcgtcac caccggtagc accaagtact tcaagaccaa taccgatggt gtagatgcca
     5101 gcgcgcaggg taaagatagc gtcgcgattg gttctggctc cattgctgcc gcggataaca
     5161 gcgtcgcact gggtacaggg tctgtggcaa ccgaagaaaa tacgatctct gtaggctcat
     5221 ctactaatca acgtcgtatc accaacgtag ccgcaggtaa aaacgacacc gatgctgtta
     5281 acgtggcaca gttgaagtct tccgaagctg gcggtgtgcg ttacgacacc aaagctgatg
     5341 gttctatcga ctatagcaat atcaccctcg gtggcggcaa cggcggtacg actcgtatca
     5401 gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ttacgcgcag ttgaagcaaa
     5461 gcgtgcagga aacgaagcaa tacaccgatc agcgaatggt tgagatggat aacaaactgt
     5521 ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc
     5581 cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact tacaacggtg
     5641 aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat
     5701 tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt
     5761 ggtaatgatc cattaagtta gtgtgactaa gggaaaacgc caaattgcct gatgcgttac
     5821 gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc tctgatgagg
     5881 attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc
     5941 ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt gccaatacat
     6001 aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc agtgccactt
     6061 ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc
     6121 aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt
     6181 tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa
     6241 ctgaaaggat acgtcgccgc ctcgtggacg gtggcaatcg cccttgccgt ggctttgctg
     6301 ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggttt cttctacggg
     6361 ttgtggccca tcgcgtggat cattattgca gcggtgttcg tctataagat ctcggtgaaa
     6421 accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga ccagcgtctg
     6481 caaatgctga tcgtcggttt ctgtttcggc gcgttccttg aaggagccgc aggctttggc
     6541 gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc
     6601 gccgggctgt gcctgattgt taacaccgcg ccagtggcat ttggtgcgat gggcattcca
     6661 atcctggttg ccggacaggt aacaggtatc gacagctttg agattggtca gatggtgggg
     6721 cggcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc
     6781 tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc
     6841 gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat ctcttcgctg
     6901 gtatcactgc tctgcctgac gctgttcctc aaacgctggc agccagtgcg tgtattccgt
     6961 tttggtgatt tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacactgcg
     7021 ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga cagctaccgt aacactgtgg
     7081 agtatcccgc cgtttaaagc cctgttcgca tcgggtggcg cgctgtatga gtgggtgatc
     7141 aatattccgg tgccgtacct cgataaactg gttgcccgta tgccgccagt ggtcagcgag
     7201 gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg caccgccatt
     7261 ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga cgctatcagc
     7321 accttcggca gcacgctgaa agaactggct ctgcccatat actccatcgg tatggtgctg
     7381 gcattcgcct ttatttcgaa ctattccgga ctgtcatcaa cactggcgct ggcactggcg
     7441 cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg
     7501 accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa
     7561 caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt
     7621 aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa
     7681 gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc
     7741 gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac
     7801 ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc
     7861 tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc ggcgtatcac
     7921 gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac
     7981 gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc
     8041 agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc
     8101 gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca
     8161 aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac
     8221 aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc
     8281 aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga
     8341 tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg
     8401 ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg
     8461 ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc
     8521 tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag
     8581 cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg
     8641 tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcact
     8701 gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc tgtttaatga
     8761 gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg
     8821 tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta ctctctcgac
     8881 ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca
     8941 gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc
     9001 gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga
     9061 tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac
     9121 gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt taggtaatat
     9181 ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa
     9241 cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc
     9301 gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc
     9361 tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc
     9421 tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag
     9481 cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact
     9541 gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct
     9601 gctaaatctg atcgagaaag agatgaaagt ggcgatgacg ctgactggcg cgaaatcgat
     9661 cagcgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagtttc ctgcggcact
     9721 ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcccc ctaaccctct
     9781 ccctcaggga gctgtctctt gatcagatct cctgatcaag agacagggag agggccgggg
     9841 tgaggggaaa aggccgcact gctcaccaat tttctgctat cctgcccccg cactaagggg
     9901 gcagtatgct aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca
     9961 tccgtctttg cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct
    10021 gggacgataa gcgcctgcgc cgcgcggggt tggactatca cgagtttacc gccgttacgc
    10081 gtcatcatga ctttcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca
    10141 ccacgaaagg tacgcctgct cacagcgccg taaactatca ggatggcgat tatctgatgt
    10201 tcggcccgga aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa
    10261 aaattcgcat tccgatggtg ccggacagcc gcagcatgaa cctgtccaat gcggtgtcag
    10321 ttgtggtgta tgaagcctgg aggcagctgg gatatccggg agcgttgttg agagattagt
    10381 tactgtatgc cggatagatg attacatcgc atccggcacg atcacagcac atcagatccc
    10441 atccccatac tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg
    10501 cttatcgctg tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg
    10561 cggcaccggt tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat
    10621 attgccgagg attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg
    10681 gtcaccacca gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt
    10741 ttcaattacc gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg
    10801 gccaattttt gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag
    10861 aaagattgcc aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg
    10921 caaggcatga aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc
    10981 gcgggtacgc accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc
    11041 gtaggcttct tccaccactt cacggatagc aatagcaggc ataattggcg atgacagctt
    11101 gttcgccagc atgtagctca gtgcactacc aaggttttcg tgcttgagta gcgtcgcgtg
    11161 gtaaaaactg gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt
    11221 gttccagaca atttccagtt cttcacacga cattgcttac tccacacgat gagataatga
    11281 ccggcccgtt ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc
    11341 tgctgcgctc gtccttgcgt gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt
    11401 ttccgcaata taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat
    11461 gcgccagttc gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct
    11521 cctgcgcgct ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg
    11581 actggttgtc ggtacaggta agcaccagat cgcccagccc ggccatgccc ataaaggtgg
    11641 caggatcggc acccagcgcc gcgccaagac gcgacatttc agccagccca cgggtgatca
    11701 gcgccgtacg cgcattcgca ccaaaaccga taccgtcgga catccccgca ccaatggcaa
    11761 tgacgttttt caccgcgccg ccaagctgca cgccaatgaa atccggattg ctgtaaacgc
    11821 ggaaactttt gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg
    11881 aggccagcga aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag
    11941 agataaccgc cagcggaatt tgatcgccta acgcctcgcg ggccacgtcc tgtaacagac
    12001 gcccggtttc cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatcg
    12061 gcggtttaat ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat
    12121 tacggctggc tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag
    12181 gcacatcggg gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag
    12241 ggtcatggcc ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg
    12301 tgccgtacga gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc
    12361 ctgatgttct tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc
    12421 gaagttaacc ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat
    12481 gcactcacga gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat
    12541 ctgggtgcct tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa
    12601 cgcggtttct tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtatacgtc
    12661 atctgccagt tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt
    12721 ctggaaaacg tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg
    12781 gatctggaaa gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt
    12841 tgtccttaaa tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc
    12901 agtccaccac gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca
    12961 ataaaaatct ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg
    13021 gcattgccat cgatcggcaa ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt
    13081 gcacgatggc aatacgggca ggtttctttg gtatagattt caacattggc cataatttag
    13141 ctcctgtttt tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc
    13201 agaagcttat aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa
    13261 agacgacagg taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca
    13321 acgccctctt tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc
    13381 tcctggcact gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca
    13441 agctcaccaa cattgttggc tttgatttca ctcggcaaca gattgataga acctgcgata
    13501 tggcctttac ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg
    13561 atcagacgcg tagcttcacc acgactaatc accttcactt tcgaggtcag gctcttaaac
    13621 gtagtcacaa gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg
    13681 ccaacaaatt gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa
    13741 aaccaggaaa ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat
    13801 gcaattttgc ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg
    13861 gtcgtatatt ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa
    13921 attttgactc ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg
    13981 gtgattctgg atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct
    14041 aaaaccccgg taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc
    14101 ggtctggaag tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac
    14161 ctgggtgccg gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat
    14221 cgcgctttct ttgctaatcc agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt
    14281 aaagcagtac acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac
    14341 atcatggcga tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca
    14401 ttccttgacg gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa
    14461 gagaaatttg ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg
    14521 atggaccgcg ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg
    14581 cagggtgagt tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac
    14641 gaaaacgacg agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca
    14701 atggaagacg gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc
    14761 actcgtgcat tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc
    14821 gatttcgtga tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc
    14881 gcttccctgg ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt
    14941 atttccgaaa ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag
    15001 tcgttcaaag gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg
    15061 caaccggaaa tgagctccgc agagctgacc gaaaaactgg ttgcggcaat caagagcggc
    15121 aaatacgaca ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg
    15181 atggaagcag cggttaaagc ggttgaagcg ctggatcact gcgtggaaga agtcgcgaaa
    15241 gcggttgagt ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag
    15301 atgcgcgatc cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg
    15361 atttacgttg gcgataagaa cgtgaaagcg gttgaaggcg gcaaactttc tgacatcgcg
    15421 ccgaccatgt tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg
    15481 ttcatcgtgg aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc
    15541 gtgaaaccgc gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc
    15601 gtattgttgt gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag
    15661 gccgatatcg ccgcgaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg
    15721 ctcgcacaat tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa
    15781 acgcaaaaca cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc
    15841 aaactggagc agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca
    15901 ttccgtcagg gcgagcatac cggtattcag ctgattctca gcggtgaaga aagccagcgt
    15961 ggacagcgtt tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc
    16021 cagttgaagc aaacgcgtga agaagtcgcc atgcagcgtg ctgaactgga agagaaacag
    16081 agcgagcaac aaacgctgtt atatgagcag cgcgcccaac aggcgaaact gactcaggcg
    16141 ctgaacgagc gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag
    16201 cagctaagtg aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa
    16261 gccgcggcga aagcgcgtgc agaacgagaa gcacgtgagg cccaggcggt tcgtgaccgc
    16321 cagaaagaag cgacgcgcaa aggcaccacc tataaaccga ccgaaagcga aaaatcgctg
    16381 atgtcccgta ccggtggtct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg
    16441 ccgacgctgc atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtg
    16501 attggtgcct ctgaaggtac tgaagttaaa gcgattgccg acggtcgggt gattctggct
    16561 gactggctgc aaggttacgg cttggtggtg gtggttgagc atggtaaagg cgacatgagt
    16621 ctttacggct ataatcagag cgcgctggtg agcgttggtt cgcaggttcg cgcgggccag
    16681 ccgattgcac tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt
    16741 cgccgccagg gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca
    16801 tttcgtcgta acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct
    16861 ggcaaacttg ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg
    16921 ctggcgatgc cctccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag
    16981 atggcgacca aagcgcataa cagcgggcac gaagtgttga ttcatctccc gatggcacca
    17041 ctgagtaaac agccgctgga gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt
    17101 gagcgcatta ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac
    17161 atgggtagca agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcactggag
    17221 cgttacaatc tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc
    17281 gcgcaaggta ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac
    17341 gaagcggaca tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca
    17401 accattgcca ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat
    17461 aacctgccgc cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt
    17521 gatacttcta caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc
    17581 gtgaagctgt gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa
    17641 gtattaagcg aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa
    17701 ggctggggca aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac
    17761 gcgtaatgcg atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca
    17821 gtaacgcctg gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac
    17881 cggaagtaaa tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcgca
    17941 ccgcatggca aacacgcaat gcttcgtaag ttatttgctg atgaaattcc ggataaatcg
    18001 taattttgtc ggcataattt cgatttaatt tctccagcag gcgggtaatc ttaatatagt
    18061 gacgttgata attaaggttt ttattccctt gtctatgcaa ccgactcact gacgtattat
    18121 gcagataata tttatataat gattgctcgg tatatcgcgc acgcagcgcg ttaaacatga
    18181 attctgttgt ccagacaata tcctgatgat gtaatccggc aataaattta atgttatttt
    18241 taacaataac atcacggcga taaaccccca tccagacaac gtgagtccaa cggcgcgaag
    18301 aaagccccat ccgcagccag tccgggccgg ttaatacgcc ggttgagcga aggcgatcgc
    18361 tggggatgga ttgccaggtt tctcccgttt cacgaaaaca ccagtcagcg ttgcactgcg
    18421 ccacgtcgag gtcatcctct aacgccatgg tcatcagcgt ttcgtacatg gtgggataga
    18481 cctcatcgtc agcatcgaca aaagcgacat atttgcccgt cgccacctcg atcccacgat
    18541 tacgcgctac cgatgcgcca gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt
    18601 ctgcgtaatg ctttgctatt tcaacagaat tatccgttga accatcgtta ataataatga
    18661 tttccagagc agtccaggtt tgcgtaatta aagattccat acaagtgcgg aaatcatcgc
    18721 ccgcattata taacggaata ataacactaa gtttattggt gctgttcatc ataaattacc
    18781 gacgacctct gaagttatcc tgacgtttta caggctttgt attaagaaac tattaagcct
    18841 gtgagatatt atttttacag gtgagagagt cacttcaggg tagagagata atgagagcag
    18901 ctatataaga gatcagcgca attacgatat ctttattaca ctgacaggat tttacatatt
    18961 taacggatta tcctcaggca aaagggctgg aataccagcc cttgttcgtg ttaatcccaa
    19021 ctcagaatta ctttcccgga ctggcccgaa cgcatagcgt caaagccctt ctggaaatca
    19081 tcgatagaga aacgatgggt aatgatcggc gagagatcca gacctgactg aatcagcgcc
    19141 gccatcttgt accaggtttc aaacatctca cgaccgtaaa tacctttaat gaacaagcct
    19201 ttaaagatta ctttggtcca gtcgatagac atatcggacg gtggaatacc cagcatcgca
    19261 atacgaccgc cgtggttcat ggtatcgagc atggtacgaa acgctggcgg cgcgccggac
    19321 atttccagac cgacatcaaa gccttcggtc atgcccagtt cagccatcac atcattaagg
    19381 ttttctttcg cgacgttaac cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg
    19441 tattcgttaa catcggtgat caccacattg cgtgcaccaa cgtgtttcgc caccgccgca
    19501 gccataatac ctatcggacc tgcaccagaa accagcacat cttcgcccac cagatcgaac
    19561 gacagcgcgg tatgcacggc gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag
    19621 atattgtctg ggattttgaa ggcgttgaac gccgggatca ccagatattc ggcgaagcag
    19681 cccggacggt taacgccgac gccgatcgtg ttgcggcaca gatgggtacg accgccacga
    19741 cagttgcgac agtgaccgca ggtgatatgg ccttcgccag aaacgcgatc gccgattttg
    19801 aagcctttca cttcctgacc aatacctacc acttcaccga catattcatg gcctacgacc
    19861 atcggcaccg ggatggtttt ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc
    19921 ccgcagatgg ctgttttacg gattttaatc agcagatcgt tatgcccgag ttccggtaca
    19981 ggaacgtcgg tcatccagat gccctcttcc gctttcagtt tggataacgc tttcatctca
    20041 catcctcagg cgataacgcc cagttgttta ccaatacgcg tgaatgcttc tactgcacgc
    20101 gtaatttgct caggggtatg cgccgcagac atctgggtac gaatacgcgc ctgacctttc
    20161 ggaacgaccg gatagaagaa accggtgacg taaatgccct ctttttgcag ctcacgggca
    20221 aatttctgcg ccactactgc atcgccaagc atgaccggaa taatggcatg atcggctccc
    20281 gccagggtaa agcccgccgc cgacatttgc tcacggaact ggcgcgcgtt cgcccacaga
    20341 cggtcacgca gttcgctgcc cgcttcgacc atctccagta ctttgatgga cgcggcaaca
    20401 atggccggtg ccagcgagtt ggagaacagg tacggacgag aacgctggcg cagccactca
    20461 accacctctt tgcgcgccgc ggtataacca ccagaagccc cgcccagcgc tttaccaagt
    20521 gtgccggtga taatatcgac ccggcccatc acatcgcagt attcatggga accacgaccg
    20581 ttttcaccga caaaaccgac cgcgtgggag tcgtctacca tcaccagggc atcgtattta
    20641 tctgccagat cgcaaacgcc cttcaggttg gcaatcacgc cgtccatcga gaacacacca
    20701 tcggtggcaa tcagcacatg acgcgcaccg gcttcacgcg cttctttcag acgagcttca
    20761 agctcctgca tatcgttgtt ggcatagcga tagcgtttag ctttacacag acgcacacca
    20821 tcaataatag acgcgtggtt cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc
    20881 gtttcaaaca ggccaccgtt agcatcaaag caggaagagt agagaatcgc atcttccatc
    20941 cccaggaatg ccgccagttt ttgttcaagc tctttatggc tgtcctgagt gccgcaaata
    21001 aaacgcaccg aagccatgcc gaaaccgtga gaatccattc ccgcctttgc cgccgcaatc
    21061 agatacggat gattcgccag cccgagatag ttattggcac aaaagttaat gacgtggctt
    21121 ccatcagcca cagtgatatc tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac
    21181 aacccttccg cccgtgcggt ttccagatcg ttggttaact gctgataaaa ttctccacgc
    21241 attgcgattc tccagacagg gcaaattcca gcacatatta cccaaactta taggtcgcga
    21301 cgagatgacg cgttaacact tctgcaaaat tcaggataaa taacgtgtaa cttgatgtct
    21361 ctcaacacct acagatagta gaaaaactgt acagttttgg ctgggcataa tgatgattga
    21421 ttttactgca ttgatataaa aagaaaaaat gatgccaaag catcattttt cctatctcag
    21481 ttggtatcaa aacgctaccc actaatggag ggaaacctat tttcttgaaa ggaaaattct
    21541 cagcgcctgc atttttgccc ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca
    21601 ttttcctctc cccagatagt ttaggttgaa gagatcgggg cgtttgtaat agcacatgac
    21661 aaaaatgccc tggtcatcat ccacaatgtt attatttagt gttatttttt gactttccag
    21721 aactaattta tagaattctt tccatttatg ctgtgaacca acaattgcgc ccccaataat
    21781 atatacatga ttgccaatca tgaaatcaaa aacctgttgt tgagatgtga cagtaagccc
    21841 tttttttata gtgaaaagat gcatcttact ttcatcaaat gggaaatccc aaattttcaa
    21901 tcctcgagtt acatttggct tacggcaata gccaaaatct atccacgcaa caagaggcgt
    21961 tttcactaag cccatgttga tagctttatt tacaaaatag gccttaaggt tacataccaa
    22021 aacatactct ggtgaccagt actctgggtt ttttaattgt cgaggctcaa gtctatttgt
    22081 aaacgactca tctttttgaa ttttttcgat acgacttctg atatatctaa atttcttttt
    22141 tatatcgata acgataaccg ttgttggttt tccgttgcga atcgcttcaa cccttggttt
    22201 caaatcggga gaagtgaaaa taatcatctc attttcaagt gcggccagac gctcaaagta
    22261 actaaaataa acatcaactg aacgagctag tttttcacgg aaccctttat tagcagtcca
    22321 atcccctcta ccaa
//
LOCUS       DAZFVO020000080        22139 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFVO020000080 DAZFVO020000000
VERSION     DAZFVO020000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 22139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.080"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1007)
                     /gene="malZ"
                     /locus_tag="ACHKNB_RS13960"
                     /old_locus_tag="pgaptmp_002792"
     CDS             complement(<1..1007)
                     /gene="malZ"
                     /locus_tag="ACHKNB_RS13960"
                     /old_locus_tag="pgaptmp_002792"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HGU4196278.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RL"
     gene            complement(1163..2536)
                     /gene="proY"
                     /locus_tag="ACHKNB_RS13965"
                     /old_locus_tag="pgaptmp_002793"
     CDS             complement(1163..2536)
                     /gene="proY"
                     /locus_tag="ACHKNB_RS13965"
                     /old_locus_tag="pgaptmp_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HGU4196279.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2612..3931)
                     /gene="brnQ"
                     /locus_tag="ACHKNB_RS13970"
                     /old_locus_tag="pgaptmp_002794"
     CDS             complement(2612..3931)
                     /gene="brnQ"
                     /locus_tag="ACHKNB_RS13970"
                     /old_locus_tag="pgaptmp_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HGU4196280.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4338..5633)
                     /gene="phoR"
                     /locus_tag="ACHKNB_RS13975"
                     /old_locus_tag="pgaptmp_002795"
     CDS             complement(4338..5633)
                     /gene="phoR"
                     /locus_tag="ACHKNB_RS13975"
                     /old_locus_tag="pgaptmp_002795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HGU4196281.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5691..6380)
                     /gene="phoB"
                     /locus_tag="ACHKNB_RS13980"
                     /old_locus_tag="pgaptmp_002796"
     CDS             complement(5691..6380)
                     /gene="phoB"
                     /locus_tag="ACHKNB_RS13980"
                     /old_locus_tag="pgaptmp_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HGU4196282.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6570..7772
                     /gene="sbcD"
                     /locus_tag="ACHKNB_RS13985"
                     /old_locus_tag="pgaptmp_002797"
     CDS             6570..7772
                     /gene="sbcD"
                     /locus_tag="ACHKNB_RS13985"
                     /old_locus_tag="pgaptmp_002797"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HGU4196283.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQQYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKTQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7769..10912
                     /gene="sbcC"
                     /locus_tag="ACHKNB_RS13990"
                     /old_locus_tag="pgaptmp_002798"
     CDS             7769..10912
                     /gene="sbcC"
                     /locus_tag="ACHKNB_RS13990"
                     /old_locus_tag="pgaptmp_002798"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HGU4196284.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQTLQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11038..12222
                     /gene="araJ"
                     /locus_tag="ACHKNB_RS13995"
                     /old_locus_tag="pgaptmp_002799"
     CDS             11038..12222
                     /gene="araJ"
                     /locus_tag="ACHKNB_RS13995"
                     /old_locus_tag="pgaptmp_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HGU4196285.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12365..13273)
                     /gene="mak"
                     /locus_tag="ACHKNB_RS14000"
                     /old_locus_tag="pgaptmp_002800"
     CDS             complement(12365..13273)
                     /gene="mak"
                     /locus_tag="ACHKNB_RS14000"
                     /old_locus_tag="pgaptmp_002800"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HGU4196286.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13398..14309
                     /gene="rdgC"
                     /locus_tag="ACHKNB_RS14005"
                     /old_locus_tag="pgaptmp_002801"
     CDS             13398..14309
                     /gene="rdgC"
                     /locus_tag="ACHKNB_RS14005"
                     /old_locus_tag="pgaptmp_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HGU4196287.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14387..14470)
                     /locus_tag="ACHKNB_RS14010"
                     /old_locus_tag="pgaptmp_002802"
     CDS             complement(14387..14470)
                     /locus_tag="ACHKNB_RS14010"
                     /old_locus_tag="pgaptmp_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196288.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(14467..14808)
                     /locus_tag="ACHKNB_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /pseudo
     CDS             complement(14467..14808)
                     /locus_tag="ACHKNB_RS14015"
                     /old_locus_tag="pgaptmp_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14956..15240)
                     /gene="ppnP"
                     /locus_tag="ACHKNB_RS14020"
                     /old_locus_tag="pgaptmp_002804"
     CDS             complement(14956..15240)
                     /gene="ppnP"
                     /locus_tag="ACHKNB_RS14020"
                     /old_locus_tag="pgaptmp_002804"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HGU4196289.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15312..15989)
                     /gene="aroM"
                     /locus_tag="ACHKNB_RS14025"
                     /old_locus_tag="pgaptmp_002805"
     CDS             complement(15312..15989)
                     /gene="aroM"
                     /locus_tag="ACHKNB_RS14025"
                     /old_locus_tag="pgaptmp_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HGU4196290.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16247..16438)
                     /gene="yaiA"
                     /locus_tag="ACHKNB_RS14030"
                     /old_locus_tag="pgaptmp_002806"
     CDS             complement(16247..16438)
                     /gene="yaiA"
                     /locus_tag="ACHKNB_RS14030"
                     /old_locus_tag="pgaptmp_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HGU4196291.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16488..17012)
                     /gene="aroL"
                     /locus_tag="ACHKNB_RS14035"
                     /old_locus_tag="pgaptmp_002807"
     CDS             complement(16488..17012)
                     /gene="aroL"
                     /locus_tag="ACHKNB_RS14035"
                     /old_locus_tag="pgaptmp_002807"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HGU4196292.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQSAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17195..17653)
                     /gene="yaiI"
                     /locus_tag="ACHKNB_RS14040"
                     /old_locus_tag="pgaptmp_002808"
     CDS             complement(17195..17653)
                     /gene="yaiI"
                     /locus_tag="ACHKNB_RS14040"
                     /old_locus_tag="pgaptmp_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HGU4196293.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17773..18582
                     /gene="proC"
                     /locus_tag="ACHKNB_RS14045"
                     /old_locus_tag="pgaptmp_002809"
     CDS             17773..18582
                     /gene="proC"
                     /locus_tag="ACHKNB_RS14045"
                     /old_locus_tag="pgaptmp_002809"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGU4196294.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18599..19714)
                     /gene="adrA"
                     /locus_tag="ACHKNB_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /gene_synonym="dgcC"
     CDS             complement(18599..19714)
                     /gene="adrA"
                     /locus_tag="ACHKNB_RS14050"
                     /old_locus_tag="pgaptmp_002810"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HGU4196295.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19816..20136)
                     /gene="psiF"
                     /locus_tag="ACHKNB_RS14055"
                     /old_locus_tag="pgaptmp_002811"
     CDS             complement(19816..20136)
                     /gene="psiF"
                     /locus_tag="ACHKNB_RS14055"
                     /old_locus_tag="pgaptmp_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HGU4196296.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20255..21670)
                     /gene="phoA"
                     /locus_tag="ACHKNB_RS14060"
                     /old_locus_tag="pgaptmp_002812"
     CDS             complement(20255..21670)
                     /gene="phoA"
                     /locus_tag="ACHKNB_RS14060"
                     /old_locus_tag="pgaptmp_002812"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HGU4196297.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21771..22031)
                     /gene="iraP"
                     /locus_tag="ACHKNB_RS14065"
                     /old_locus_tag="pgaptmp_002813"
     CDS             complement(21771..22031)
                     /gene="iraP"
                     /locus_tag="ACHKNB_RS14065"
                     /old_locus_tag="pgaptmp_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HGU4196298.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         5565 a         5937 c         5522 g         5115 t
ORIGIN      
        1 agccgccagc cgtccatact ccacggcgct ttcagccaat ggcggacaat actgtcttcc
       61 ccgcgataaa tttcattcac cagactttcc gactgataat ccagcttcgg caagctggca
      121 tagccaagcc agtcgagcgc cgtgccatca tcactaaacg agtaccagtc gcgccagggc
      181 gattcagggt tgtgacaagc tccccccgtg ccacgattgt gcctgtcaaa ccaggcatgg
      241 gaatcgccac tgtggttaaa cacgccatcc agcaccagcc gcattcccag ctgctgcgta
      301 ttgtggcgta agcgcagcaa cgccccatca ccgccaaact gcggatcgac atggcgataa
      361 tcctcggtat cgtatttatg tacgctggga gctttaaaca ccggattgag atacagcgcc
      421 gtcacgccaa gctttttcag atacggcagt ttttcgctta tcccgtccag atcgccgcca
      481 tagaacgttg atccgcccgc ctgcgccgtg accggttcat cccagtcacg caagatgatc
      541 tcttgtccgg ctgcatgatg gtaatagaca tgatcctgtt cagcttcacg aggaagacta
      601 cgcgcaaaac gatcagggaa gatctgataa aaaatctgat ccgcagccca ttgtgggccg
      661 atatccggta catcgacggc aaactgctcc agtcgtgccg gcggcattcg gctgaagccc
      721 tgcggtgtaa accaacgctg gcgatcgtgc cacagcagtt tgaaactgta acgccgccgg
      781 ggttgtccgc tggagagatc aatcgccgca cgccatgcgg tgacgccagg ctgctgctga
      841 ctgcgctgct tatgcatcga tactgacatt tcttcgttat cgtgttctgt acgcagcata
      901 atgcgctgcg gtgggtcttc gcccgtcagc cacagtgtaa tgagcagttg atctttgctt
      961 tgtttaacaa atgggggcac cggcaggtgc catgcattta acatcataat tcccctgtga
     1021 tgaaaatctg atcaccttgc catagcctaa tgcaggatca ctcatcatct gaaggatatt
     1081 tgggggagga ggacgggggc ggataacttt cggaatagcc ggatcaggat ttcctcctga
     1141 accggcttac agaacgcaag gattactggt tttcagccag ctgacgatcg tggcggcgtt
     1201 taaacatcca gccaatcaac agcacaacaa tccacgcgaa accgacatac agcgagatac
     1261 gcgtatccgg gtgataacca atcaacccga taataaagag caggaaaatc aaaccgccga
     1321 tggtcgttgc taccccaccc ggcactttaa atttcagcgc cttaacttct tctggcggca
     1381 aacggcgacg gaaggcaatt tgcgacagca ggatcataat ccacacccac accgtggcga
     1441 aggttgccag cgaagcgatc accaggaaga cgttttccgg catgatgtag ttcagataca
     1501 ccgcaaacag cagcgcggta gtcatcacca gcaccgtaac ccacggaata ccgcgacgcg
     1561 acgttttgct gaaaattttc ggcgcgctgc cctgctctgc cataccgtgg agcatacggc
     1621 ctacgccaaa tacgtcactg ttaattgccg acagcgaagc agtcagcaca acaaagttaa
     1681 gaatgctggc ggcaaaggta atgcccatat gctggaacgt cagcacgaac gggctaccgg
     1741 cagtgccaac ctgattccac gggtagatag acataatgac gaacagcgta ccgacgtaga
     1801 acaccagaat acgcatcggc acggagttaa tcgcacgcgg tatcgatttc tcaggatctt
     1861 tcgcttcacc ggcggtaatc ccgataattt cgatcccacc gtaagcaaac atcaccattt
     1921 gcaacgacat caccatccca agccagccgt tactgaagaa gccgccgttg ctccacaggt
     1981 tatgaatacc ggtcggttgc ccgccgttgc caatccccca gatgatgatg ccgaaaccgg
     2041 cgacaatcat gatgatgatg gtggcgactt taaagaacga gaaccagaat tccagctcac
     2101 cgaagacctt cacgctcatc aggtttacgg cgcaaatgat cagcaccacg ctcagtaccc
     2161 aaatccagtg cggcaccgtc gggaaccaga cacccatata gataccaaaa gcggtcacat
     2221 cggcgatggc gacaataagg atttcaaagc aataggtcca gccggtaatg taacctgcca
     2281 gcgggccgag gttttcctgc gcataacgcg agaaagagct agctgccggg ttatgtaccg
     2341 acatttcccc cagcgcacgc ataatgatat acgccgcgat accaccgata atataggcca
     2401 acaacacgct cggaccggcc attttgatgg cgtctgccga accgtaaaac agcccggtgc
     2461 caattgctga acccagtgcc ataaagcgta tgtggcgggt acttagccca cgctttagct
     2521 tgttcttact ttccatcaat tcctaaccca tcaacacaat aaaaaaccac ggggcatcgc
     2581 agccccgtgg ttaaaacaaa tgttcagtga tttagtgagc gctggaggtc acctgacgac
     2641 ctgccgcgcg atcccagata atggccagaa ccaccatcac cactgttggc attaaccacg
     2701 ccagaccttg ttcggccagc ggtaaacgct gcgcccagga cggtaagata tcgctgaatg
     2761 cagatgcttt gatcccgtcg agaataccaa aaagcaggct gataaacatc ggcggagcaa
     2821 tcacgcggga cgaattatgc caccatgagc gtgtaaaact taatacaacc agtgcgatac
     2881 acggcggata aatagcggtc agcaccggta cggagatctg gatcagctgg cttaagccga
     2941 ggttagaaac caccatcgag aagccgccga ggataaacac cagcgtacga taagagagcg
     3001 gtacgtactg ggcaaagaat tctgcacaag cacaggtcag gccaactgcc gttaccaggc
     3061 aggcgatgaa gattaacgcc gccagcagga agctaccgcc gccgccaaag gtgtgctgaa
     3121 cgtaagcatg cagaatagca gcgccgtttg cagactgatc gaccagcgac gcgctgtctg
     3181 aacccagacg gaacagcgcc aggtacagca gagtcagacc aacacccgcc atcaggccag
     3241 cccagacggt ataacgggtc agcagacgcg cttcggtaac gccacgagaa cgcgccgcgt
     3301 taacaataac gataccaaac accattgcgc ccagcgtatc catggtcaga tagccgttaa
     3361 cgaagccgtt agaaaacgca gcgttttgat aagcctcagt cgccgtgctg atagaacccg
     3421 ccggccagac aatcgcggca acagacagga tgaccagcgc gataattttc agcggcgcaa
     3481 ggaagttgcc cacggtatcc agcagcttgc ccggatagag cgaaaccaga ataacgatag
     3541 cgaaatagac caggctgtag ataaacagcg gcagcgcgga atcaccagtc agcggcgcaa
     3601 tccccacttc aaaggaaacg gtagctgtac gcggcgtagc gaaaagcggc cccaccgcca
     3661 ggtaacaaac cgttgccagc agtacgccag cgactttacc gattggcgtg ctgaggctgt
     3721 caacaccgcc gccaactttt gccagcgcca ctaccgttag taccggcaga ccaacggcag
     3781 taatgaggaa gccgaatgcc gcagtccaga cgtgttcgcc tgcctgcaag ccgaccattg
     3841 gagggaaaat aatgttacct gcgccgacga acaacgcaaa tgtcataaag cccagagcga
     3901 tgatatcgcg cgatcttaat tgatgggtca taaatcatac tgcctgtgga tgtggtgttg
     3961 taacgttaaa tttttgtgcc aatccctggg gatgatacag cggtatattt aaacaatttt
     4021 agcgggaaat actcccaacc aggcgcggcg aagaatagtt tttcgattta ccgcgttaat
     4081 gcagtcagta tgacagcacc tgaagcgcaa tttaaacgct tataacgttt taaagcaagg
     4141 agggatctaa aaaccagagg tatatagcga taagaaatca ccaaccagta taaaacacta
     4201 tttccgtgaa aaagacatgg ctaatcatgc gaacaaaaaa tcaccaaccg ttgtaaaatc
     4261 taacgcccgt attttgactg caaagaaaat aagccagcat cggctggctt atggcaataa
     4321 aagatgacaa aggcggatta atcgctgttt ttggcaatta aacgttccgg gataacaaaa
     4381 ctgaaacgtg ttccttttcc tactgtactc tcaatattca ggcgactttc gtgatgattc
     4441 accgcatgtt tcacgatcgc taaccctaat ccgctaccgc cggtttgccg ggaacgcgct
     4501 ttatcaacgc gataaaaacg ctcggtcagg cgcggaatat gctccggtgc aatgcccggt
     4561 ccgttatctt caacgctaaa ttcggcaccg tgcggcactc gctgccagcg tacggtgata
     4621 tgcgtgcctt ctggagtatg attcacggcg ttatagacca ggttcgaaat cgcactgcgt
     4681 agctgatctt cgttgccaga cactttgagg ccgttatcta tctcaaaggt aaatgtctgt
     4741 tttttctgac tcagagtctg agcctcacgc tcaacaacgc gcagcatcat cggcacatca
     4801 accttttcat tgagcaaatg cgtcggtgcg gcttctattt tcgacagcgt cagcaactgc
     4861 ttcaccagcc cttccatccg ctgggtctgc tcgcgcatgg tgtgcaacgc tttttcgcgt
     4921 accgcgcctt ccagcggctg ctcatccatc atctccaggt aaccctgtaa cacggtcaat
     4981 ggcgtgcgta actcatggct cacgttggca aaaaagttac gccgcgcccc ttccagttga
     5041 tgcatttgcg tgacatcacg cgccaccatc agcaactgtt tgtgggtata aggcatgacg
     5101 cgaatttcca gatgccgccc ggtgttgagc accagattga gcgggcgaga aaaatcacgc
     5161 gttttcagat attgcgtaaa ctccgggtaa cgcagtaggt taaggatgtt ctgcccgtta
     5221 tcttccggcc agcgcaaacc aagaatttgt tgcgccagac cgttacacca gaaaataccg
     5281 ccctcttccg tggtcagcac caccgcgtcg ggcagcgact ccgcgccgct acgaaagcgt
     5341 ttaatcagat tgcccagttc acggcggcgt tttttatttc gcagctgcat ctggtgtaag
     5401 ccgtatagta gcggttccca gctaccacgc cccggtggcg gggtcatact gcgatccacc
     5461 cacagccacc atgaaaggcg caataaattc cagaaatgcc agataagcag tcctgttacc
     5521 gatgccagca aaaaccaggg caggtaacca aaaaatgcac ccaggatgaa agccgggagg
     5581 cagcaaagta gcagctccag caccagcctt ttccacgaca gccgttccag cacgtaagat
     5641 actccagtta agaaatcata agccctgctc tgcgtccgat gagcaaggcg ttaaaagcgg
     5701 gttgaaaaac gatatcctgt accgcgcacg gtctgcacca tgcggtcatg cccgccgggc
     5761 tccagtgctt tacgcaggcg acgaatgtgg acatcgaccg tgcggtcttc cacatacacg
     5821 ttagttcccc agacgtggtt taacagctgc tcgcggctgt acacgcgctc aggatgcgtc
     5881 ataaaaaagt gcagcagttt aaattctgtc ggccccatct ccagcggctc ttcgcccgcc
     5941 atcactcggt gagatgtcgg gtcgagactt aatccctgca tctcaatcac ctcttccacc
     6001 gccattggcg aaatacggcg cattaccgct ttgattcgcg ccaccagctc cttcggcgaa
     6061 aacggcttgg tgatatagtc atccgcgccg gtttcaaggc cgcgcacgcg atcttcttct
     6121 tcccctctgg cggtcaacat caccactgga atatcccggg tcatcgactc gcgcttgagg
     6181 tgtttgatga actggatacc ggagccgcca ggtaacatcc agtcaaggag aattaaatcc
     6241 ggccagggtt cattcagttg attcacagca ctgtcataat cttccgcttc gaccggctga
     6301 aagccatttt gttcgagcac gaagcagacc atttcgcgaa ttggagcttc atcttctacg
     6361 accagaatac gtctcgccat gatttgccct gttgtaataa ataggttgcg atcattaatg
     6421 cgacgtcatt atgcgtcaga tttatgacag atttatgaaa agctcgtcgc acatatcttc
     6481 aggttattga tttccgtggc gcagaaaaaa gcaaatggca catctgtttg ggtataatcg
     6541 cgcccatgct ttttcgccag ggaatcgtta tgcgcatcct tcacacctca gactggcatc
     6601 tcggccagaa cttctacagt aaaagccgcg aagctgaaca tcaggctttt cttgactggc
     6661 tgctggaaac agcacaaacc catcaggtgg atgcgattat tgttgctggt gatgttttcg
     6721 ataccggctc gccgcccagt tacgcccgca cgttatacaa ccgttttgtt gtcaatttac
     6781 agcaaactgg ctgtcatctg gtggtactgg caggaaacca tgactcggtc gccacgctga
     6841 atgaatcgcg cgatatcatg gcgttcctca atacgaccgt ggtcgccagc gccggacatg
     6901 cgccgcaaat cttgcctcgt cgcgacggga cgccaggcgc agtgctgtgc cccattccgt
     6961 ttttacgtcc gcgtgacatt attaccagcc aggcggggct taacggtatt gaaaaacagc
     7021 agcatttact ggcagcgatt accgattatt accaacaaca atatgccgat gcctgcaaac
     7081 tgcgcggcga tcagcctctg cccatcatcg ccacgggaca cttaacgacc gtgggtgcca
     7141 gtaaaagtga cgccgtgcgt gacatttata ttggcacgct ggacgcgttt ccagcacaaa
     7201 actttccacc agccgactac atcgcgctcg ggcatattca ccgcgcacag attattggcg
     7261 gcatggaaca tgttcgctat tgcggctctc ccattccact gagttttgat gaatgcggaa
     7321 agagtaaata tgtccatctg gtgacatttt caaacggcaa attagagagc gtggaaaacc
     7381 tgaacgtacc ggtaacgcaa cccatggcgg tgctgaaagg cgatctggcg tcgattaccg
     7441 cacagctgga acagtggcgc gatgtatcac aggagccgcc tgtctggctg gatatcgaaa
     7501 tcactactga tgagtatctg catgatattc agcgcaaaac ccaggcatta accgaatcat
     7561 tgcctgttga agtattgctg gtacgccgga gtcgtgaaca gcgcgagcgt gtgttagcca
     7621 gccaacagcg tgaaaccctc agcgaactca gcgtcgaaga ggtgttcaat cgccgtctgg
     7681 cactggaaga actggatgaa tcgcagcagc aacgtctgca gcatcttttc accacgacgt
     7741 tgcataccct cgccggagaa cacgaagcat gaaaattctc agcctgcgcc tgaaaaacct
     7801 gaactcatta aaaggcgaat ggaagattga tttcacccgc gagccgttcg ccagcaacgg
     7861 gctgtttgct attaccggcc caacaggtgc ggggaaaacc accctgctgg acgccatttg
     7921 tctggcgctg tatcacgaaa ctccgcgtct ctctaacgtt tcacaatcgc aaaatgatct
     7981 catgacccgc gataccgccg aatgtctggc ggaggtggag tttgaagtga aaggtgaagc
     8041 gtaccgtgca ttctggagcc agaatcgggc gcgtaaccaa cccgacggta atttgcaggt
     8101 gccacgcgta gagctggcgc gctgcgccga cggcaaaatt ctcgccgaca aagtgaaaga
     8161 taagctggaa ctgacagcga cgttaaccgg gctggattac gggcgcttca cccgttcgat
     8221 gctgctttcg caggggcaat ttgctgcctt cctgaatgcc aaacccaaag aacgcgcgga
     8281 attgctcgag gagttaaccg gcactgaaat ctacgggcaa atctcggcga tggtttttga
     8341 gcagcacaaa tcggcccgca cagagctgga gaagctgcaa gcgcaggcca gcggcgtcgc
     8401 gttgctcacg ccggaacaag tgcaatcgct gacagcgagt ttgcaggtac ttactgacga
     8461 agaaaaacag ttacttaccg cgcagcagca agaacaacaa tcactaaact ggttaacgcg
     8521 tctggacgaa ttgcagcaag aagccagccg ccgtcagcag accttgcaac aggcgttagc
     8581 cgaagaagaa aaagcgcaac ctcaactggc ggcgctcagt ctggcacaac cggcacgaaa
     8641 tcttcgtccg cactgggaac gcatcgcaga acacagcgcg gcgctggcgc atactcgcca
     8701 gcagattgaa gaagtaaata ctcgcttaca gagcacaatg gcgcttcgcg cgagcattcg
     8761 ccaccacgcg gcgaatcagt cagcagaatt acagcagcag caacaaagcc tgaatacctg
     8821 gttacaggaa cacgaccgct tccgtcagtg gaacaacgaa ctggcgggtt ggcgtgcgca
     8881 gttctcccaa caaaccagcg atcgcgagca tctgcggcaa tggcagcaac agttaaccca
     8941 tgctgagcaa aaacttaata cgcttgcggc gatcacgttg acgttaaccg ccgatgaagt
     9001 tgctagcgcc ctggcgcaac atgccgagca acgcccactg cgtcagcgcc tggtcgcgct
     9061 gcatgggcag attgttcccc aacaaaaacg tctggcgcag ttacaggtcg ctatccagaa
     9121 tgtcacgcaa gaacagacgc aacgtaacgc cgcacttaac gaaatgcgcc agcgttataa
     9181 agaaaagacg cagcaacttg ccgatgtgaa aaccatttgc gagcaggaag cgcgcatcaa
     9241 aacgctggaa gctcaacgtg cacagttaca ggcgggtcag ccttgtccac tttgtggttc
     9301 caccagccac ccggcggtcg aggcgtatca ggcgctggag cctggcgtta atcagtctcg
     9361 attactggcg ctggaaaacg aagttaaaaa gctcggcgaa gaaggtgcgg cgctgcgcgg
     9421 gcaactggat gcattaacga agcagcttca gcgcgatgaa aacgaagcgc aaagcctccg
     9481 acaagatgag caagcactta ctcaacaatg gcaagccgtc acggccagcc tcaatatcac
     9541 cttgcagcca caggacgata ttcaaccgtg gcttgatgca caagatgagc acgaacgcca
     9601 gctgcggtta ctcagccaac gacatgaatt acaagggcag attgccgcgc ataatcagca
     9661 aattatccag tatcaacagc aaattgaaca acgccagcag cagctcttaa cggcattggc
     9721 gggttatgca ctgacattgc cacaggaaga tgaagaagag agctggttgg cgacacgtca
     9781 gcaagaagcg cagagctggc agcaacgcca gaacgaatta accgcgctgc aaaaccgtat
     9841 tcagcagctg acgccgattc tggaaacgtt gccgcaaagt gatgatctcc cgcacagcga
     9901 agaaactgtg gcgctggata actggcggca ggttcatgaa caatgtctcg cattacacag
     9961 ccagcagcag acgttacagc aacaggatgt tctggcggcg caaagtctgc aaaaagccca
    10021 ggcgcagttt gacaccgcgc tacaggccag cgtctttgac gatcagcagg cgttccttgc
    10081 ggcgctaatg gatgaacaaa cactaacgca gctggaacag ctcaagcaga atctggaaaa
    10141 ccagcgccgt caggcgcaaa cactggtcac tcagacagca gaaacgctgg cacagcatca
    10201 acaacaccga cctgacgggt tggctctcac tgtgacggtg gagcagattc agcaagagtt
    10261 agcgcaaact caccaaaagt tgcgtgaaaa caccacgagt caaggcgaga ttcgccagca
    10321 gctgaagcag gatgcagata accgtcagca acaacaaacc ttaatgcagc aaattgctca
    10381 aatgacgcag caggttgagg actggggata tctgaattcg ctaataggtt ccaaagaggg
    10441 cgataaattc cgcaagtttg cccaggggct gacgctggat aatttagtcc atctcgctaa
    10501 tcagcaactt acccggctgc acgggcgcta tctgttacag cgcaaagcca gcgaggcgct
    10561 ggaagtcgag gttgttgata cctggcaggc agatgcggta cgcgataccc gtaccctttc
    10621 cggcggcgaa agtttcctcg ttagtctggc gctggcgctg gcgctttcgg atctggtcag
    10681 ccataaaaca cgtattgact cgctgttcct tgatgaaggt tttggcacgc tggatagcga
    10741 aacgctggat accgcccttg atgcgctgga tgccctgaac gccagtggca aaaccatcgg
    10801 tgtgattagc cacgtagaag cgatgaaaga gcgtattccg gtgcagatca aagtgaaaaa
    10861 gatcaacggc ctgggctaca gcaaactgga aagtacgttt gcagtgaaat aactattcag
    10921 caggataatg aatacagagg ggcgaattat ctcttggcct tgctggtcgt tatcctgcaa
    10981 gctatcactt tattggctac ggtgattggt agccgttctg gtagttgtga tggtggtatg
    11041 aaaaaagtca ttttatcttt ggctctgggc acgtttggtt tggggatggc cgaatttggc
    11101 attatgggcg tgctcacgga gctggcgcat aacgtaggaa tttcgattcc tgctgccggg
    11161 catatgatct cgtattatgc gctgggagtg gtggtcggtg cgccaatcat cgcactcttt
    11221 tccagccgct actcactcaa acatatcttg ttgtttctgg tggcgttgtg cgtcattggc
    11281 aacgccatgt tcacgctctc ttcgtcttac ctgatgcttg ccattggtcg cctggtttcc
    11341 ggctttccgc atggcgcatt ttttggcgtc ggcgcgatcg tgttatcaaa aattatcaaa
    11401 cccggaaaag tcaccgccgc cgtggcgggg atggtttccg ggatgacagt cgccaatttg
    11461 ctgggcattc cgctgggaac gtatttaagt caggaattta gctggcgtta caccttttta
    11521 ttgatcgctg tttttaatat tgcggtgatg gcatcggtct atttttgggt gccggatatt
    11581 cgcgacgagg cgaaaggaaa gctgcgcgaa caatttcact ttttacgcag cccggccccg
    11641 tggttaattt tcgccgccac gatgtttggc aacgcaggtg tgtttgcctg gttcagctac
    11701 gtaaagccat acatgatgtt tatttccggt ttttcggaaa cggcgatgac ctttattatg
    11761 atgttagttg ggctagggat ggtgttggga aatatgctaa gtggcaggat ttcaggacgt
    11821 tattcaccac tgcgcattgc agcagtgact gactttataa ttgtactggc actgctgatg
    11881 ctctttttct gcggcggcat gaaaacaacg tcgcttattt ttgcttttat ttgttgcgcg
    11941 ggattatttg ccctttcagc acctctgcaa atattattac tacaaaatgc caaaggcgga
    12001 gagttattag gtgccgcagg tgggcaaata gcgtttaacc ttggtagcgc cgtcggcgca
    12061 tattgcggtg gtatgatgct gacgctgggg ctggcatata attacgtggc gctgcctgcc
    12121 gccctgcttt cgtttgctgc gatgtcgtca ttgctgctgt atggtcgcta taagcgccag
    12181 caagcggcgg atagtccggt gctggcgaaa ccactggggt agattgcagt cacgttgcgc
    12241 gttgcttatt gccggatgcg gcgtgaacgc cttatccgcc ctacgcggtt ccggcacatt
    12301 ttgtaggcct gataagacgc ggcaagcgtc gcatcaagca tgatgcgcca attgcctacg
    12361 ttttttactc ttgtggccat aaccacgcag cgccgcgtac gccgctggaa tcaccgtgct
    12421 tcgccttacg caccggcgtt tcacattcgc cgccgaagac aaattgttta atcaactgcc
    12481 caaccgtttg atataaacgg tctacattgc tcatcccgcc ccccaggaca atcacatccg
    12541 gatcgagaat attcacgaca tgtgccagcg attttgccag ccgcagctcg tagcgacgca
    12601 atgccagttc cgctaccgga tcgctttctt caaccaggcg gataatttca ctgcctttca
    12661 gcgcatgtcc gctcaaacga cgataatcag tcgcgaatcc cgtgcccgaa ataaaggttt
    12721 caatacaacc ttgtttaccg caataacaag ggacttcctc gcgataacgc agttcgtctt
    12781 cgtccatcca cggtagcgga ttgtgtcccc actcacctgc cgtgccattg ccgccgatat
    12841 gcgcccgccc attgaatgcc acgcccgcgc cgcatcccgt gccgataatc acggcaaata
    12901 ctgtctgcgc tcccgctgcc gcgccatcta ctgcttctga aaccgccaga cagttggcgt
    12961 catttgccag ccgcacttcc cgctgcaacc tcgcgcttaa gtctttatcg aatggctgac
    13021 cgttgagcca ggttgaattg gcattcttca ccacaccggt gtaaggcgaa attgagccag
    13081 gaatgcccat acctaccgtt ccgcgctgcc ctgtcgcctg ctccgccata tcaaccaacg
    13141 tggcgatcgt ttcaatagtt tgccggtaat catcacgcgg cgtgggcaga cgatggcggt
    13201 acaactgctc ccctgcatcg cccagtgcaa tcacttcagt tttggtgccg cctaaatcga
    13261 tacctatacg cacggtactc tccttatttt tttcaatatc aatagcgtag agacggacaa
    13321 ccggattggc aatgcaaggc cgcctacaat tcgttatcat gcccgctaaa tttaacgaca
    13381 aggccgtgga aattatcatg ctgtggttca aaaatttaat ggtttaccgt cttagccgcg
    13441 agatttcgct gcgtgcagaa gagatggaaa aacagctagc ctcgatggca tttaccccat
    13501 gcggcagcca ggacatggcg aagatgggct gggttcctcc gatgggatcg cacagcgatg
    13561 cgttaacgca cgttgccaat ggtcaaattg ttatctgcgc gcgcaaagaa gaaaaaatcc
    13621 tcccgtctcc ggtgattaaa caggcgcttg aagcgaaaat cgccaaactg gaagcggaac
    13681 aggcgcgtaa gctgaagaaa accgaaaaag attcgctgaa agacgaagtg ctgcactctc
    13741 tgctgccgcg tgctttcagc cgttttagcc agacaatgat gtggatcgac acggttaacg
    13801 gtttgattat ggtggactgc gccagtgcca aaaaagcgga agatacgctg gcattactgc
    13861 gtaaaagcct ggggtcgtta ccggttgtac cattgagcat ggaaaacccg attgaactga
    13921 cgctgaccga atgggttcgc tccggtagtg cggcacaggg cttccagctg cttgatgaag
    13981 ccgagctgaa atcgttgctg gaagatggcg gcgtgatccg cgcgaagaaa caagatctga
    14041 ccagcgaaga gatcaccaat cacattgaag ccggaaaagt ggtgactaaa ctggcgctcg
    14101 actggcagca gcgcattcag tttgtgatgt gcgacgatgg ttcgcttaag cgtctgaagt
    14161 tctgcgacga gctgcgcgat caaaacgaag atatcgaccg tgaagatttc gcccagcgtt
    14221 ttgatgccga tttcatcctg atgactggtg aactggcagc gttaattcaa aacctgattg
    14281 aaggattagg tggcgaagca caacgttaat tgctgatttt cctttaatgc cggatgcgac
    14341 gcctgccgcg tcttatccgg cgtacgaagc cacaccaggc atataattat tcgctacggc
    14401 gggcaataat ttttagcgca gcaatattat gcgttttatg ctgtaacttg ctccatggac
    14461 gttgtgtcat tgtttttcct caagccagtg acgcgcttca ctgcgaattt tccgcattgc
    14521 cccggttttc actaaatccc gtaacagcga aagatcgggt tgttcgacaa ataagagtaa
    14581 tgatggatcc tcttttaacc acgtcgtttt cacatcggca gcgagctgtg gattattcat
    14641 agctagcgac cgatgccgag actcggtcaa tgtcgttaac aacgacgccg gggtatgtgc
    14701 gttgcgtaat gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca
    14761 ttcatcatct gatttatctt ttatcgtagc cagttccgca acaatatctg gagatcttca
    14821 ctgatattta tatcaacctt gatgaattat gaatatatcg gaaattgaaa aaatcgcagc
    14881 aaaggaaata ttattatggg ttaaagagag gatatttcag aaaaatgccc tcccgttcaa
    14941 ggggaaggcg aggaattaca gatagcggca cagataagag gtgggttcgg caacttgcag
    15001 atgaaactca ctgtgaccgg gaacattaaa caccgaaccg gcttcataca cctgccagtc
    15061 ggtcgcgtca ggcagtaaca cattcagcgc gccactgatt accgtcatct cttccggctc
    15121 agcggtgctg aaggtgtatt cgccttcaac cataacaccc acgctggcgc gaccagtgct
    15181 gctgctggaa aagccgattg atttcacttt gccggaaaag tactcattac tttgaagcat
    15241 aaactggccc tgtctggtag ttgaacatct acttcactat aggggccaga ggcgctggct
    15301 gtcacgcaaa attacaccag taattccgca gccagccgtg caatcaatac gttagacagc
    15361 aagacaggaa catcgagctg tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa
    15421 caatccagca tgatgacatc agcccctttt gccagtaatt ctttcccggc atcaatgatt
    15481 ttttgttctg aatcatgaat ggggttgccc aatgaaaata ccggcgattt ctgcaaaatt
    15541 tgccattttt gcgcctgaac ggtcagcatc tcctcaaccg gaacgataac ccccacctga
    15601 tgatcttcaa caatagagga aaccagtgga ggcaatattc gcgacggctc aagaaagatc
    15661 gtattacgcg cagtcatact actaatgttt gctgtactca ttaataaaat gacgtcataa
    15721 ccctgattat cgagcacttc aaccacacct tgcaggtctc gctccacttt acgacgcgaa
    15781 acatgggcca gctggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc
    15841 gcgtactccg ccatcacttc ttcacgactt aacttcccca gcaagctatg atgggaaata
    15901 ttatcttcgt caatgtattc cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg
    15961 atggtcagga tcgccaacga cgcactcatt gttcatccac cttacttttc tttcgtcgtt
    16021 aattaccggg caagtgtgaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa
    16081 agcgtagcag cagacgtgca cggcataatg taaagattcc aaatgattcc agtaatggat
    16141 tgttattgtt taatattcta attattagaa aaacatgaat tatgaaaaat gtgacgcaga
    16201 tcccttgtag tggcactcag aatcccttcc gagcagtctg atgcggtcac tctttgtcag
    16261 ggtcctcata gcgttttttc gcatccatcg cttcctgagc cgtcggatgt tcactgatca
    16321 acgagtctgg tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggct
    16381 ttcctttttc aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg
    16441 ttcttcctta tttcacggga tgaacgttaa gtataggcgc tctaaaatca acaattgatc
    16501 gtctgtgcca gggcgctacg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg
    16561 atgataatat gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact
    16621 tcttcgctca gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagattgc
    16681 agtcggttaa ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc
    16741 atgaagtgac gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat
    16801 ggcgcagtta ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc
    16861 ctttcgacga tctccgcgac cgtcatattg agctgtgatt gcaaccactg atcggtatcg
    16921 acaaaccgac ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc
    16981 cgaggcccga tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg
    17041 cggcgaaagc cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt
    17101 gtaaagaaaa aattccactt aaagtgaaaa tctcaataca tcccttacta taccaataaa
    17161 tattcaagaa tgaagtgtaa ataataaatt acatttagcc acgactacgt tgcacttcca
    17221 gccaccactt ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa aggctatctg
    17281 gtccgccggt ctggatccca ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc
    17341 gctcacgaat ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc gctgcgcctt
    17401 tctcgatggc ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg cccgcttcac
    17461 actgccggac aatttcgtta tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa
    17521 taaatcgcga tggcggcacg cgtaaactct ggtttgctac cagtaccagc ggcatctgca
    17581 tacgttccgc cgcgcgatac aaaatctctt taattacatt gggacacgcg tcggcatcca
    17641 cccaaattgt cataaagtca tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc
    17701 ttccggcata gttctgttta tgcttctgcc agcgattatc aaaacaatga atttcacggc
    17761 aggagtgagg caatggaaaa gaaaatcggt tttattggct gcggcaatat gggaaaagcc
    17821 attctcggcg gtctgattgc cagcggtcag gtgcttccag ggcaaatctg ggtatacacc
    17881 ccctccccgg ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg
    17941 gcgcaagaag tggcgcaaat cgccgacatc atttttgccg ccgttaaacc tggcatcatg
    18001 attaaagtgc ttagcgaaat cacctccagc ctgaataaag actctctggt cgtttctatt
    18061 gctgcaggtg tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatt
    18121 cgcgccatgc cgaacactcc cgcgctggtt aatgccggga tgacctccgt aacgccaaac
    18181 gcgctggtaa ccccagaaga taccgctgat gtgctgaata ttttccgctg ctttggcgaa
    18241 gcggaagtaa ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca
    18301 gcctacgtat ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca
    18361 cgcgcccagg cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg
    18421 gaaacgggag aacatccggg ggcactgaaa gatatggtct gctcaccggg aggcaccacc
    18481 attgaagcgg tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg
    18541 acgaagtgta tggaaaaatc agaaaaactc agcaaatcct gatgactttc gccggacgtc
    18601 aggccgccac ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg
    18661 ccgatttcaa ccactcacga tagtgactca tttgtgggtt cagcggcgca acccccacac
    18721 taatccgtaa agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca
    18781 cccgtaacat ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt actgcaaact
    18841 catcgccgcc aaaccgacca atcacatcgc taccgcgcag ggtaatttgt aactgtcggg
    18901 taagcgccac aatcgcttca tcgcccacat catgacccca ggtatcgttg atgctcttga
    18961 aatggtcgat atcgataatc agtaacgttg catcgcgatt atgccgccga cagttatcaa
    19021 attcattgcg taacatagtt tcccaatgac gtcggttata cacgcccgtc atgccgtcgc
    19081 gggtactcat gacctgcaac ctgcgtttat gttccgccag tttggttgcc gtctggtagc
    19141 tgacccagcc aaacagcaga ggataaacga caataatggg aagggagagc caccattcca
    19201 gcggcgcact attgaacgac acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa
    19261 ccaccatcaa caccagaccc gcgacaaaca gacgggggcc gcctgccccc atcaaattca
    19321 gacacataat catcaacata gcggtggaag gcagcacgtt tacgcccatt acgcctaccc
    19381 acattcccgc taatactgca tcggttttta agttgtaaat ttcccggcta agcggatcga
    19441 cggccctgct cgctatctgc caggctaaat gcggccagac gaacgcccag ccgaccaaca
    19501 ccagccacca ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca
    19561 tgccagccag gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc
    19621 gctgatgttc gttttgagga gttaaaggag gttcctcccc atgcgccgcc gcttttttga
    19681 aaaagttttc atcattcatt atttttggga acattcagaa acaattttcc caaattatag
    19741 agacggggct aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta gctcacccgt
    19801 tagcactatc aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc
    19861 atcacctttc agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg
    19921 tggcgtcaaa cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta
    19981 ggtcttacga gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggatgtcat
    20041 acgctgttgt tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag
    20101 accaaaaagc aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta
    20161 accatgagcg tatgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc
    20221 acccggcagc gaaaattcac tgccgggcgc ggttttattt cagccccagg gcggctttca
    20281 tggtgtagaa gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat
    20341 acgccgcaat acgcaactga ctgccggtat gttcttgtga atcctcttcg gagttcccgt
    20401 agctcatcac catcactgcg ccatctttgg tatttagcgc ctgggtgagg cccggagctt
    20461 tggtatccgg cgcgacaatc tggctggcgt gggcgtgatc agcggtgact atgaccagcg
    20521 tattgccatc ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg
    20581 tctcgccaat ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt
    20641 caacttgcag gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca
    20701 tctgcgccag ggttggtaca ctgtcattac gttgcggatt aggcgtacag gtgactgcgg
    20761 gcttatcgat attgccgtgg tacgttgctt tcggtcctag ccagcgcact ggcatattgc
    20821 cgtcagcaaa cagtccaagc agaggttttt gctgattcgc ttccgtcacc gaattcagtg
    20881 aggcagcatc gctgaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc
    20941 cctgccattc accggcggtt gccgtttcag caaaggtttt tgcgccaccg ccaagcgtaa
    21001 cgtcggcacg agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag
    21061 cgttacccgg acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat
    21121 gtgccaccag cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg
    21181 ccagacctgc ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga
    21241 cgcccagcgc gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg
    21301 aatcggtgac gtagtccggt ttgccggttt ttttattcag cgcatagtga gtgtattgcc
    21361 cggtaagcgg taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac
    21421 gtgcggctgt aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg
    21481 caggtttatc gctaagagaa tcacgcagag cggcagtctg atcacccgtt aaacggcgag
    21541 caccgccggg tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg
    21601 gtgtccgggc ttttgtcaca ggggtaaaca gtaacgataa gagtgccagt gcaatagtgc
    21661 tttgtttcac tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta
    21721 taagtctcgg ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga
    21781 ggatgcttca ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg
    21841 ctggcatcgg gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg
    21901 tcattttgcg ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc
    21961 gcacacagtt ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg
    22021 agatttttca tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac
    22081 aacttcatag tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatat
//
LOCUS       DAZFVO020000081        21953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFVO020000081 DAZFVO020000000
VERSION     DAZFVO020000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.081"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..528)
                     /locus_tag="ACHKNB_RS14070"
                     /old_locus_tag="pgaptmp_002814"
     CDS             complement(<1..528)
                     /locus_tag="ACHKNB_RS14070"
                     /old_locus_tag="pgaptmp_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4196299.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDA"
     gene            complement(620..696)
                     /locus_tag="ACHKNB_RS14075"
                     /old_locus_tag="pgaptmp_002815"
     tRNA            complement(620..696)
                     /locus_tag="ACHKNB_RS14075"
                     /old_locus_tag="pgaptmp_002815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:tct)
     gene            complement(710..786)
                     /locus_tag="ACHKNB_RS14080"
                     /old_locus_tag="pgaptmp_002816"
     tRNA            complement(710..786)
                     /locus_tag="ACHKNB_RS14080"
                     /old_locus_tag="pgaptmp_002816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(750..752),aa:Arg,seq:tcg)
     gene            complement(796..871)
                     /locus_tag="ACHKNB_RS14085"
                     /old_locus_tag="pgaptmp_002817"
     tRNA            complement(796..871)
                     /locus_tag="ACHKNB_RS14085"
                     /old_locus_tag="pgaptmp_002817"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(836..838),aa:Ile,seq:cat)
     gene            complement(912..1970)
                     /locus_tag="ACHKNB_RS14090"
                     /old_locus_tag="pgaptmp_002818"
     CDS             complement(912..1970)
                     /locus_tag="ACHKNB_RS14090"
                     /old_locus_tag="pgaptmp_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4196300.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(2121..2318)
                     /locus_tag="ACHKNB_RS14095"
                     /old_locus_tag="pgaptmp_002819"
     CDS             complement(2121..2318)
                     /locus_tag="ACHKNB_RS14095"
                     /old_locus_tag="pgaptmp_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4196301.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2545..3366)
                     /locus_tag="ACHKNB_RS14100"
                     /old_locus_tag="pgaptmp_002820"
     CDS             complement(2545..3366)
                     /locus_tag="ACHKNB_RS14100"
                     /old_locus_tag="pgaptmp_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4196302.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDCVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVSKFDIEEAWAERQLKKVTR"
     gene            complement(3363..3743)
                     /locus_tag="ACHKNB_RS14105"
                     /old_locus_tag="pgaptmp_002821"
     CDS             complement(3363..3743)
                     /locus_tag="ACHKNB_RS14105"
                     /old_locus_tag="pgaptmp_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4196303.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYLRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GKPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(3744..4802)
                     /locus_tag="ACHKNB_RS14110"
                     /old_locus_tag="pgaptmp_002822"
     CDS             complement(3744..4802)
                     /locus_tag="ACHKNB_RS14110"
                     /old_locus_tag="pgaptmp_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4196304.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISSTQERE
                     LSLAELSWWAVCNQVVDALPEAVVRRSLGLPAEKIRSVYRESDIIPGEQAATSILKQR
                     TKNIALPLHVHQQQNPPQEKTLVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            complement(4804..5082)
                     /locus_tag="ACHKNB_RS14115"
                     /old_locus_tag="pgaptmp_002823"
     CDS             complement(4804..5082)
                     /locus_tag="ACHKNB_RS14115"
                     /old_locus_tag="pgaptmp_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196305.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            5218..5475
                     /locus_tag="ACHKNB_RS14120"
                     /old_locus_tag="pgaptmp_002824"
     CDS             5218..5475
                     /locus_tag="ACHKNB_RS14120"
                     /old_locus_tag="pgaptmp_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4196306.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            5481..5780
                     /locus_tag="ACHKNB_RS14125"
                     /old_locus_tag="pgaptmp_002825"
     CDS             5481..5780
                     /locus_tag="ACHKNB_RS14125"
                     /old_locus_tag="pgaptmp_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4196307.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(5983..6513)
                     /locus_tag="ACHKNB_RS14130"
                     /old_locus_tag="pgaptmp_002826"
     CDS             complement(5983..6513)
                     /locus_tag="ACHKNB_RS14130"
                     /old_locus_tag="pgaptmp_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4196308.1"
                     /translation="MSTITREWLQQAINDYESVRDELPFGLDDYQGNILASLRIALAS
                     LEAEPVAWKVTFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAKPVP
                     VTPDGWISCSERMPDTKTAVLVAREFDRKGDWRMKWATYIPGHPDANDGWVVPGASWI
                     PSHWMPLPEPPQEVRQ"
     gene            complement(6510..7187)
                     /locus_tag="ACHKNB_RS14135"
                     /old_locus_tag="pgaptmp_002827"
     CDS             complement(6510..7187)
                     /locus_tag="ACHKNB_RS14135"
                     /old_locus_tag="pgaptmp_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4196309.1"
                     /translation="MSKIDYQALRAKAEKATCGEWSLEYGEGRFDGDDALIHREVAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELEAAEKRIAELQARDVKPVAWMRNANVTSFMSRFTTDEKYAVEQ
                     WGDDAVALYPLPVAFIPACFTDERNLMHINERGRETSLIWSKQNSDSGDIKLFRIAAA
                     AGKGEES"
     gene            complement(7184..7747)
                     /locus_tag="ACHKNB_RS14140"
                     /old_locus_tag="pgaptmp_002828"
     CDS             complement(7184..7747)
                     /locus_tag="ACHKNB_RS14140"
                     /old_locus_tag="pgaptmp_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4196310.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVIAEEVGRDGVLVILEAMKATGNMPATDAFLAEVRAQGVEM
                     FAECAYTLEHHDHAVAFAAELRKGGNQ"
     gene            complement(7734..8039)
                     /locus_tag="ACHKNB_RS14145"
                     /old_locus_tag="pgaptmp_002829"
     CDS             complement(7734..8039)
                     /locus_tag="ACHKNB_RS14145"
                     /old_locus_tag="pgaptmp_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4196311.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(8036..8317)
                     /locus_tag="ACHKNB_RS14150"
                     /old_locus_tag="pgaptmp_002830"
     CDS             complement(8036..8317)
                     /locus_tag="ACHKNB_RS14150"
                     /old_locus_tag="pgaptmp_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4196312.1"
                     /translation="MILRQCAGTMTVESIGSLIGRSGAAVRTKARELGICMMLRGDYH
                     QSAKCSQRDIELARQLHQRGVPRREIAEKFGMKLGAVNNYVYFDRRVQA"
     gene            complement(8350..9111)
                     /locus_tag="ACHKNB_RS14155"
                     /old_locus_tag="pgaptmp_002831"
     CDS             complement(8350..9111)
                     /locus_tag="ACHKNB_RS14155"
                     /old_locus_tag="pgaptmp_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4196313.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAVPVTENDGKA
                     FPQPAGVALPVQESATQEDIKTETVADIVQSLPSFTETQADELIFLSLCRANLALRRA
                     KSDVQKWERVCAALRELNKHRDILRDITATRELLR"
     gene            complement(9145..9606)
                     /locus_tag="ACHKNB_RS14160"
                     /old_locus_tag="pgaptmp_002832"
     CDS             complement(9145..9606)
                     /locus_tag="ACHKNB_RS14160"
                     /old_locus_tag="pgaptmp_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4196314.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPKGPTPIEWLKQEYERRKAAGFI"
     gene            complement(9599..10636)
                     /locus_tag="ACHKNB_RS14165"
                     /old_locus_tag="pgaptmp_002833"
     CDS             complement(9599..10636)
                     /locus_tag="ACHKNB_RS14165"
                     /old_locus_tag="pgaptmp_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HGU4196315.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARMRESAPTGESNGASLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAIQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTF
                     EALDCPDWCDD"
     gene            complement(10705..11130)
                     /locus_tag="ACHKNB_RS14170"
                     /old_locus_tag="pgaptmp_002834"
     CDS             complement(10705..11130)
                     /locus_tag="ACHKNB_RS14170"
                     /old_locus_tag="pgaptmp_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4196316.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(11114..11386)
                     /locus_tag="ACHKNB_RS14175"
                     /old_locus_tag="pgaptmp_002835"
     CDS             complement(11114..11386)
                     /locus_tag="ACHKNB_RS14175"
                     /old_locus_tag="pgaptmp_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4196317.1"
                     /translation="MLDSTRKKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVECQANTQPETGVIHENQA"
     gene            11495..11896
                     /locus_tag="ACHKNB_RS14180"
                     /old_locus_tag="pgaptmp_002836"
     CDS             11495..11896
                     /locus_tag="ACHKNB_RS14180"
                     /old_locus_tag="pgaptmp_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4196318.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVTGHPLHWFLLPPEEGEQMFTPNTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            11924..12115
                     /locus_tag="ACHKNB_RS14185"
                     /old_locus_tag="pgaptmp_002837"
     CDS             11924..12115
                     /locus_tag="ACHKNB_RS14185"
                     /old_locus_tag="pgaptmp_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HGU4196319.1"
                     /translation="MSTALSPIVSEFETIEQENSYNEWLRAKVASSLADPRPAIPHDE
                     VIAEMENLIAQIAVTNRSE"
     gene            12115..12402
                     /locus_tag="ACHKNB_RS14190"
                     /old_locus_tag="pgaptmp_002838"
     CDS             12115..12402
                     /locus_tag="ACHKNB_RS14190"
                     /old_locus_tag="pgaptmp_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4196320.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHARRQFPFSI"
     gene            12676..12828
                     /locus_tag="ACHKNB_RS14195"
                     /old_locus_tag="pgaptmp_002839"
     CDS             12676..12828
                     /locus_tag="ACHKNB_RS14195"
                     /old_locus_tag="pgaptmp_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4196321.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            13347..14198
                     /locus_tag="ACHKNB_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /pseudo
     CDS             13347..14198
                     /locus_tag="ACHKNB_RS14200"
                     /old_locus_tag="pgaptmp_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            14209..14397
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS14205"
                     /old_locus_tag="pgaptmp_002841"
     CDS             14209..14397
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS14205"
                     /old_locus_tag="pgaptmp_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4196322.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            14394..14582
                     /locus_tag="ACHKNB_RS14210"
                     /old_locus_tag="pgaptmp_002842"
     CDS             14394..14582
                     /locus_tag="ACHKNB_RS14210"
                     /old_locus_tag="pgaptmp_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4196323.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            14675..17119
                     /locus_tag="ACHKNB_RS14215"
                     /old_locus_tag="pgaptmp_002843"
     CDS             14675..17119
                     /locus_tag="ACHKNB_RS14215"
                     /old_locus_tag="pgaptmp_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4196324.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LYEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNHNQQETEQ
                     KGHKAEQHSPNAQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQ
                     ETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREI
                     TSTSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVD
                     ISTIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVI
                     LRRSYERQEIPCPWRYTNDRDVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAK
                     YVSAIWQKLFPNQADF"
     gene            17178..17381
                     /locus_tag="ACHKNB_RS14220"
                     /old_locus_tag="pgaptmp_002844"
     CDS             17178..17381
                     /locus_tag="ACHKNB_RS14220"
                     /old_locus_tag="pgaptmp_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HGU4196325.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCRNTLPPREAEPDFGAI"
     gene            17381..18406
                     /locus_tag="ACHKNB_RS14225"
                     /old_locus_tag="pgaptmp_002845"
     CDS             17381..18406
                     /locus_tag="ACHKNB_RS14225"
                     /old_locus_tag="pgaptmp_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4196326.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNHHDHAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVL
                     ADSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPVPVFIAMEIAYLCAARISDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWTPRLQSVIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNLAGQIS
                     GIATDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKISPTLDVPLPQN
                     IPTKYSK"
     gene            complement(18459..18548)
                     /locus_tag="ACHKNB_RS14230"
                     /old_locus_tag="pgaptmp_002846"
     tRNA            complement(18459..18548)
                     /locus_tag="ACHKNB_RS14230"
                     /old_locus_tag="pgaptmp_002846"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18512..18514),aa:Ser,seq:cga)
     gene            18642..19439
                     /gene="mtfA"
                     /locus_tag="ACHKNB_RS14235"
                     /old_locus_tag="pgaptmp_002847"
     CDS             18642..19439
                     /gene="mtfA"
                     /locus_tag="ACHKNB_RS14235"
                     /old_locus_tag="pgaptmp_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HGU4196327.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            19540..19615
                     /locus_tag="ACHKNB_RS14240"
                     /old_locus_tag="pgaptmp_002848"
     tRNA            19540..19615
                     /locus_tag="ACHKNB_RS14240"
                     /old_locus_tag="pgaptmp_002848"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19573..19575,aa:Asn,seq:gtt)
     gene            19928..21953
                     /gene="yeeJ"
                     /locus_tag="ACHKNB_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /pseudo
     CDS             19928..21953
                     /gene="yeeJ"
                     /locus_tag="ACHKNB_RS14245"
                     /old_locus_tag="pgaptmp_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         5580 a         5766 c         5094 g         5513 t
ORIGIN      
        1 tgcatctctg gtcatggcat ttccactaaa ctccattaac gccagatatg atgaaaccag
       61 tgagtgacga ctgatttgca ttccggaacg ttccagcgct gcgacacgtt gcagagtggt
      121 ataactgctg tccgttgtca tggaaacagt tgtcacaccg ggcactgata tatgtgcaaa
      181 atcagaaaat ctgtagaaag tatttgttgc cgtattaacg aatccggcca catataaatt
      241 attttgctca acaatcagac gaagatggtc aaaacgcgcc tgatagacat caagccctcg
      301 tatatccaca gcaaaataac tgcccggtgg ggtatggtta ataacagaca ccgatgtggt
      361 cccctgagat atatgctcaa gaggagtcga tatttctgtc cgtatactat ttaacgaaga
      421 gacataactt tgttgagtcg aaaagtctat cgtaaattcc cgggaatagg ataccgaaga
      481 aaagcccagc aacaggcaca gtatccattt taacaatata cacttcatat acaggtgttc
      541 cttttggctg aagtaatacg gcaccagacc cggcgcagat ataaaaaagg cccgcaaaag
      601 cgagccagaa aaataagtat ggcgcgttgt actggattcg aaccagtgac cgattgctta
      661 gaaggcaatt gctctgtccg gctgagctaa caacgctgaa taccgataat ggaccgccat
      721 cgaggactcg aaccccgcgc aaccagcttc gaaggctgac gctctgtccc gatgagctaa
      781 tggcggtatg tgatatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      841 tcgctcgctc tcaccactga gctaaagggc cgggtgcagg ataataacgg tacgtaacta
      901 atcctgcaat atcatccgtt ctgactggct aaatcctgaa cttccctgac cgtctgctca
      961 aaacgttcag tctccagctc aacaccagtt gcacgacgcc ccagtgccat cgcggctttg
     1021 actgtcgaac ccgaccccat gaaaaaatct gcaaccaggt cccccggacg actgctcgcg
     1081 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagtac
     1141 tgcaccggtt tatgcgtcca cacatcggtg tacggcacct gcgccgtcac gccaaaatac
     1201 cgccgcagat gcttatattc actctgcagt tccgcatact gccggttcag tgaagtatac
     1261 gtctccagca gctggtggtg gggcttctcc agttcacccc gctgatgctt ctcttctgcc
     1321 acccgggcaa acagcgcctg taatttcaga taatcacttt cgttcggcag ttgccactga
     1381 ctggcactga accagtgcga caccatgttt ttctttcctg tggcatccac tatctgtttt
     1441 gccgttatcc ccagggcagc acgcgcatca cgaaagtaag caatcagcgg agccatcaca
     1501 tgctgtttca gtgccctgcc cttcgcctca tacccggcat ctttcggacg atacggcccc
     1561 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgccct caggctttcc
     1621 ttgttgcacc cgttccagcg tccggacggc tttgcccaga taatatggtt cagcacactg
     1681 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatggccaca gaacaggtaa
     1741 agacttccgg caggtttcag cacccgccag aactgcgcaa gacactggtc cagccacttc
     1801 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1861 ggcggatccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1921 cagtcggcgt tgattaactc acaactggat atttttacag tattagccat agatcaataa
     1981 gcacttctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     2041 tgatccggac gcatgagcag atggctggtg agtgccccta acacccacca gccgcccatt
     2101 taccacaaat aaaaaagcct tcaggactga aggcgtctgt aacaaccgaa ctgatagtct
     2161 gccagacccg ccataacaag ctgggtcagt attaactgac agcgttcgcg tgaaaggtac
     2221 gtattctgcg caatctcccc gactgtcgcc ggttcggtga cgcttaattc attaaacacc
     2281 actctggcgg tttcggtcat atcctgctgt ttcagcatgt ctttttccct tttccggtta
     2341 acgtgacaca ccaataactc ttgtcgaaaa agccagcaag ctgaaagacc ggtattcacc
     2401 gccgccagcg cgtttactgt actggagtga tttcagtcat aaaaaaaccc gcctggcgac
     2461 gggtgtaaaa agtcttctaa cgtcaggcat aaaacgccca tcgttagggc aaatttacca
     2521 cagattccgg aaaaatcaac cttgttacct ggttaccttt tttaactgcc tctcagccca
     2581 tgcttcctca atatcaaact tgctcaccag cgcatcatag aatttcttaa ctgttttttc
     2641 ccatgacgcg cgtgttatct ggtttgtcac ctcgcatata gcattaaatg cctctgttga
     2701 cggcaatctt tcatagccac gaccaccaca acgctggcag tccctgataa caggcatacc
     2761 ccgtttttca gactcttcac gatgaatggc aacaccgcgc ccacgacaat ctttacaggc
     2821 ggtggaaacc tccccctttc cgccacactc cggacaggca acttttacca cctccctgac
     2881 ttttttccat tcctcccagt aagacggata cacgcctttt gtgcactttg cccacactgg
     2941 cggcttacca tccggatact ggatcttgtt tgtaaaaacc tcgctttcaa taaatttttt
     3001 tccatgacaa caggagcact gttttttgct cgccgcgcta cgggcataat cttcaaacgc
     3061 atacgaagcc ataatacgca tcactgccgg ttttatttct gccgggagtt ttcttaacgc
     3121 cgccacgcaa tcacaccgac tgagtgcata ttctgtcagc aattctgttg cccgttctct
     3181 gtcattcata ctaatgccca ttttcccaag aaacgcagaa aaccccatct cagcccgatt
     3241 ctgtgtcatg ccctgcgcgg ccatcacatc agtgatactc agcgcatctt tcgacgttga
     3301 ggccgatgca tcagtcaggc cgggggattt tggggagtag tatttcggta aatcttccag
     3361 tttcattttt tgacctgccc ttcaagcatt atggggtaaa tcttcacccc cagccgtcca
     3421 ccagatactg gcttgccacg tacaatattg atttcatcaa actgctcgtc atcaataagc
     3481 actcccgcat gcgtcagcgc atccagcggt gctttcagaa tattgtccag gtcacgacga
     3541 cgcttatccg gtggctctgc aataattttt attgccagcc gtccggacag gcttaatttc
     3601 agccgctgct ggcgaacaat aagcgccact gcccggagat agcgctttcc ctcctccgat
     3661 ataaaatatg tgctgccacg gcgtcgccag taagtgttca ccgtcggcgg gtaaggcaaa
     3721 acaaattcta tgcgctcggt catttatgct ttccacttca gaacacccga atttctcgcg
     3781 tgcattaaaa aacgaatcag caacaacagc tgactgccgt gtttctcttc aaaatctttt
     3841 accccggcgt gtagttcgtt atggcattta cggcataacg gaataacaaa caaatcgtca
     3901 gcctttgttc ccatacctcc cagtccatga ccaatgatgt gatgcggatc atctgcctga
     3961 ttgccacacg tcatgcattt ctgcgttttt acccagcgcg tgtatacagg catctcttcc
     4021 cgttgtggtt tctggcgctg gaggtactga gccggagact ccggatcaac ggcgatgctt
     4081 actaacgtct tttcctgggg gggattttgt tgctggtgga cgtgaagtgg cagcgcaata
     4141 ttttttgtgc gctgcttcag tatgctggtg gcggcctgct ctcccggtat gatgtcgctc
     4201 tcacggtata cggagcggat tttttccgct ggtaatccca gcgaacgacg cactactgcc
     4261 tcaggtagtg catccaccac ctgattgcag accgcccacc aggataattc ggccagcgat
     4321 aactccctct cctgcgtact gcttattgcg tgacggatga cgtcaatcat ccaggcaacc
     4381 agattctgct gagcaagttg atcgagtgat tctgatgtct ggtcgcgcag ctggttgtca
     4441 cagtgccagc acaacaccat cgcgccggta ccgtaacggt gaatgacggt ttcgctgtga
     4501 tgataatcac cgtgtggcca ctggcaggat ttcacgtgac gtaataacca gtcagacagt
     4561 gcacctgcac cacctgctgc acgaataacc cgctcatcgc tgaaaaatgg cagtaatgtt
     4621 ttatcctctg ccagcggctg gcgaacggca ggaacgactc cggacggcag accgcgcatg
     4681 tttttcggtt ccggttccac caatattctg ccgttatgga atgctgacat tgattcacgg
     4741 cccggcttaa ggagcaccat cccgagttcc ggtaccagaa caggtcgaag taatacccgc
     4801 acgttacctc cagatgcgct gctggaatgt gcgggacgga cgcggtgggc gttcggaata
     4861 agggagcctg acggagatta tccagtgacg acgatcgaag ctgagatctt tctgaaactc
     4921 gtaaccacgt ctgcggtagc actggatcag ccattcggcc tgttcttcag tgcatgggtc
     4981 atgctggaac cagtcagatt tgaatgcatg agaacgccgc ccgtgcctgc tggcaggggc
     5041 ggtggagtta tccgaattgt gtaatttggt atcttgcgcc atcggttgtc tctgctggcg
     5101 cagcaggtgc cagttgttca ggctggcgtg cggcaatatt gtctctgatt tctgttgtcg
     5161 tcaacaggca gcgtgctatc atcgaatagt gttctatcct actccgtgag gtttaccatg
     5221 cgtacaaccc aacaattcag cattacatta actaacgaga tggctgacat ggtgcgcgcc
     5281 cgtgtggctt ccggcgccta tgcttcagaa agcgaggtca ttcgtgaagg gcttcgcgca
     5341 ctgaatgagc gcgataaagc aatcgaagcg tggttaacgc attcagccgc cccctctctt
     5401 gattctatcc gcgaaaaccc aaacaacgga cgctccattt cacaggttcg cgtcgcgatt
     5461 cgatccggga agtaatctgc atgacatatg aagtcatcat tactcctgag gccgaacaac
     5521 aaataatcaa cctgcacaga tatataacgg agaaagcagg gaacatcatt gctgacaatt
     5581 atgccaatgc gcttcttgat tatcttgatg ggttttctac attcccgcat cggggcaata
     5641 aacgcgatga tattcgccag gggatgcggg taactcattt ccgccacaga acgattattg
     5701 cttttgccgt tgatggcaga aaagtcttta ttgtcggtat ctatcatggt gggcaaagtt
     5761 atgaaaccga tttcttataa acttttaccc acatcattcc ggtgttagaa taaaccgtct
     5821 gcccctctct tactgcggat tcgtaggcta tataaatcaa agatcccggc tcatgtttgt
     5881 ggcgggatct tttttcggcg atttatcccc agcggcaaat cgaatacacc accagcgcca
     5941 ccgccattgc aataccaaca tttgagaagg cctcaggcca gctcattggc gcacctcctg
     6001 cggcggttct ggtagcggca tccagtgtga tggtatccac gacgcaccag gtactaccca
     6061 cccatcatta gcgtcaggat gccccgggat gtaagtcgcc catttcattc gccagtcacc
     6121 tttcctgtca aactccctgg caacaagaac ggctgttttg gtatccggca ttcgctcact
     6181 acagcttatc caaccatccg gagttaccgg cactggcttg gcggtataaa gcggtgttat
     6241 atctgcccga aaattacatg ctttatgcag ccgcacccac cgttcgactt ctgctttgtc
     6301 agaatacata ccagtgaacg tgttatattc acggtcaatt tgcgtgaatg ttaccttcca
     6361 cgccaccggc tctgcttcca gagatgccag tgcgatacgc agggaagcca ggatattccc
     6421 ctggtaatca tcaagcccga aaggaagctc atcacgaacg ctttcataat cgttgatagc
     6481 ctgctgcagc cattctcttg tgatagtgct catgattcct ctcctttacc ggctgcggcg
     6541 gcgatacgaa aaagtttaat gtcgccgctg tctgagttct gcttactcca gataagtgac
     6601 gtttctcgcc cgcgctcgtt gatatgcatg agatttcttt cgtctgtaaa gcatgcaggg
     6661 atgaaagcta cagggagtgg atagagggca actgcatcat caccccattg ctcaactgca
     6721 tatttctcat cggttgtgaa cctcgacatg aatgatgtga cgttagcatt cctcatccat
     6781 gcaactggct taacgtcccg cgcctgcagc tcagcaatcc gcttctctgc tgcttccagc
     6841 tcaacacgca gcttccctac cgttagcgca atatcctcgt tctcctggtc gcggcgtttg
     6901 atgtattgct ggtttctttc ccgttcatcc agcagcgcca gcacggtagc tggattggct
     6961 gcggcgatga attcagcatt ggcctgctgt tccatttgga aatcttcatc gaaaccgctt
     7021 tcaggatgcg ctccttcaat tctgcaaatg ggaatatatc cagcaacttc acgatgaatt
     7081 agcgcatcat caccatcaaa tcgtccctct ccatattcga gcgaccactc gccacacgtt
     7141 gctttttctg ccttagcacg caatgcctga tagtcaatct tgctcactgg ttgcctcctt
     7201 tgcgaagctc agcggcgaag gctactgcgt gatcatgatg ttcaagtgtg tatgcacact
     7261 ccgcaaacat ctccacgccc tgcgctcgta cttcagccag gaaagcatcg gtggcaggca
     7321 tatttcctgt tgctttcatg gcctccaaga taaccagaac gccatctcgc ccaacctcct
     7381 cagcgataac ctcggtgttg tcgccaacaa catcgcagaa tgcctgaact gctttacgag
     7441 caagtgcatt ctccgccgcc agcgccacaa gattagcttc cagctctgca atacgtttgc
     7501 tttgggcttc ccgttcatcc agcagtgcct gtactacttc agggttgaaa gctgcaataa
     7561 attctgcgtt gttctctgca tttttctggt catcaaagcc aggccatttg ataatgtctc
     7621 cgcaacgttc atcccccggc gtatgcaccg catatgtatc agtgcccggc gaaatgaatg
     7681 cgacccattc gccctgggtt gccagttttg ctatctcacg tagcgtctga taattaattt
     7741 ttctcactgg ttgcctcctg gaaaataacc gcatgtccca gtttctccgc cagtgccagt
     7801 tctgccttag cgcctgccga ccgctgccag cctttcagca tgtaaatcgc atccacgcag
     7861 cgtatcatcg ccatgcaaat atccatgtag tgtggctgtg tcagtccgtc cggaagtact
     7921 gccgggttta aaacggtatg cccttcccgt ttcagtgctt cttccgccct gtgaaacgcc
     7981 tcacggttga aattttcata tcccgtcatc ggaccggcga tataaattct caccctcacg
     8041 cctgaaccct cctgtcgaaa taaacgtagt tattcactgc gcccaacttc atcccaaact
     8101 tttcggcaat ttcccgtcgg ggtacgccac gctgatgcag ttgccgcgcc agctcaatat
     8161 cacgctgtga acatttggct gactggtgat aatcaccccg taacatcatg cagataccca
     8221 gttcccgcgc tttcgtcctg acggcagccc cactacggcc aatcagactg ccgatgcttt
     8281 cgactgtcat cgttcccgca cactgccgga gtatcataat ttccgcccag cgccacttct
     8341 tccagccact caccgcagca gctctctggt ggcggtaata tcccggagaa tatccctgtg
     8401 tttgttcagt tcccgcagcg cggcacagac tcgctcccac ttctgaacat cacttttcgc
     8461 cctgcgcagc gccaggtttg ccctgcacag ggacagaaaa atcagctcat ctgcttgcgt
     8521 ttcggtaaac gatggcaacg actgcacaat gtccgccact gtttctgttt taatatcttc
     8581 ctgtgttgca gattcctgta ctggtaacgc aacacctgct ggctgaggaa aggccttacc
     8641 atcattttcc gttaccggca cggctttcgg ctctgctggt aaattatcgc ccggcatgca
     8701 gtaacgaaat ttaccgttct gatttacgcg tgccaggcgt cccgttgcgg ttaccaccgc
     8761 caacgtggaa gcaaccttgc gaatgctaac accgaactta tccgccagtt cctcacacgt
     8821 tttagccccc tcctgaccga taaactcaat catcatgtct gcggtaactt tttgttcgac
     8881 ctccccggtc agcacatccg gtacttcaga ctgttctggc tgctcttcgg ttaccccgga
     8941 ttcaccttcg ccagccagaa accagatgtg acccgcttta tcaacaacgc catttctttt
     9001 gagttcccac agttcgttga gaacctcttc acgactgata tcaagccgct cagccagtcc
     9061 aacagagctg gcttttccca ttgctttcag tgcgtcaaaa acggtttcca ttaaaatttc
     9121 ctcctacaaa atcgcttctc agattcaaat aaaaccagct gccttccggc gttcgtattc
     9181 ctgtttcagc cattcaattg gcgttggccc tttcgggtgt ttcgcccctt ccagttgtcg
     9241 tcgcactggc ggaacactca tcccgttacc aacatgcttt gcccatttcg tcagttgccg
     9301 ttccgcaagt cgttttaact caccctgcgt catctggcgc tcaatccctc tggtacgcat
     9361 ttcgaggcag atgtggtaca gcacaggctg tggccacggg tatttatcac tcccgtcgta
     9421 tcgccaggat tcattgcgcc agcgccggta ctcttccatc acggcatcca ccgtaagacc
     9481 aaatggattt gccccactct ccgaaatcag cgcaacaaac tcagcaaggt ccgggggcca
     9541 cgtttcaccc gcccggcagc ggtccatgca ctgacgacag acctgccgga tttgctgttc
     9601 agtcatcgca ccaatctggg caatccagag cttcgaaggt gcggccccgt tcttctgagt
     9661 ccagcggttc gaatacacct cccccataag ctcccacagc ttccaggccg tttccgttgc
     9721 tgataaatcc gttgtctcgt tcccactgtt cgcgtgctgc ccggatttcc tgaattgccc
     9781 gtgatgccgt gccacctgat gctgcatggc ttaccccctt gctgactggt tttacctgtg
     9841 ccctgacgtg ctgcacgtgg cgggcaaatt tctgctccca ctgaacctgc gtgaaaacct
     9901 tcccctccgc catccagtaa tcccggaatg cggcaagctc tgcaggtgta aattccggct
     9961 caggcagagc cataccccac actgctgccc gttgtcgaaa atccggcgac ggctgccaga
    10021 cagtggtcat cgaaaatttc ccgatcggtt cgctcaggcc gtccaggtat tcaggttcgg
    10081 ctgtctgcaa cgacgcacca ttcgactcac cggttggagc actctcgcgc atgcgcgcgt
    10141 tatgtgtggg gtttaattct gtatctgtat ctttatctgt cgtgacttgt cgtgacagat
    10201 gcgtgacacg tcgtgactca tcgtgacaat cagcatcata tttccgcagc ttttcgcgct
    10261 cccgctgcgc tctcttgcgc tctgccgggg attttgccgt ttgcgaaacg ttaccattgt
    10321 cctctttcag cacctgacgt ttttcccatc cggaaataag gtcaccatcc aaaacccgcc
    10381 cctgcattgc atgcaaaatt gaatcaatta cgtcttccgt cacatcaagc gcacttgcta
    10441 aatcttccgt cgtgacatca atgtgaccac gtagtgacac gccgtgacat gtcgtgacat
    10501 ttcgtgacgc gctcaccaga aggtggatat acactgccat cactgttgcg attggctgtc
    10561 ctgataccct ggcaatcgtt cgccacttgg ggtcatttgg catgtcatgc cacaatctga
    10621 gccaggcatt agccatactc acctcttctg ataccgaact ttacccacga acttccggaa
    10681 gaaatccggt ataaatattg ttggtcaatg cacaacaaca gcattaccag gctgaccacc
    10741 actgttagtc agggtgcccc aggcgatcgc tacagcgaca aaatcatcca catctttcac
    10801 cagccgatcc cgtcgttcga cgatctcccg gtaatattca gaactgtgac tgcgcatacg
    10861 ggccaccagc aaaggcggca tcgccttttc gatcgccggt aacagcgcct gaattttttc
    10921 aacagcatca gacgtgtcct tctccaccca gcggaaaatt ttctgggtat tacgagccag
    10981 ggcttccgga tggctgtcgt cgtacagttc cgggaacgtc atccccagct cgaaataagt
    11041 ccgggctatt tcagctgcgg gaactttctc accatcagga tatgcccagg cattcatcgc
    11101 catacggatg tgttcatgct tgattttcat gaatcacccc cgtctctggt tgtgtgttag
    11161 cctgacactc gacaggtaaa ccgtcggtcg gattagggta aatgtctggg cgaatttcat
    11221 gcggggtaac ctcccacttc attagctgac atagcggaat tacctgcttt gggggaacgc
    11281 caaagctaaa ccattgccaa actgtctgtt gagcgacccc catataacga cctatttcag
    11341 cctgagtgta tttctgccta atttttttgc gagtgctatc tagcattttg ccctcctcta
    11401 aaaactatag gcaaaggcta caataaaaaa ctgtacgcga tcaacagttt tttattgtga
    11461 tgcttttaac agtatttacc tgtaaaattg aataatgatg agcgccctag aagtatcgat
    11521 gtacagaatc agcaagcttc ttcaggaaac tggatggagc caggctgagc tggcccgtag
    11581 aattggtgtg acacaacaaa ctgttcaaca atgggtcagc ggtaaggcta cacctaaagc
    11641 ctcaagtttg gataaactgg ttgaggttac agggcatccg ttgcattggt ttttattgcc
    11701 tcctgaagag ggggagcaaa tgttcacccc taacacgatg aaaattggtc ctcgccaacg
    11761 cgaattactc caggctttta gtgcgttccc tgaggaggac caagaaaaaa tgcttcaaga
    11821 aatcaaagac aaaaaaaaat caatggaaga aaccattgcc cggtggctgg cggcacaaaa
    11881 aagccgccgg gcgtgaccac agtacaagaa gaggagttat gccatgagta cagccctttc
    11941 tccgatagtt tcagaattcg aaactatcga acaagaaaac agttacaacg aatggttgcg
    12001 cgctaaagta gcgtcaagcc ttgcagaccc tcgccccgca attccacatg acgaggtaat
    12061 agctgaaatg gaaaatctta ttgctcaaat tgctgtaact aacaggagcg agtaatgttg
    12121 cccattttat ggctaccatc tgctcgcgat gatttgcgtc agatcataac ttacatcgcc
    12181 aaggagaacc caccggcagc gcgtagactt aaaatacgta ttgaaacatc ggtattacct
    12241 ctatctgagc atccgtacct atatccacca agcgaacggg tttctgggtt gagagagata
    12301 gtgacccacc ctaactacat aatcctgtac agagtagctg cttcaagcat tgagattgta
    12361 agcgtgacac atgctcgccg acaatttccc ttctctatct gagctgaact attttcatac
    12421 tccctctttc gagggatttt tttgcttaag ggcacaatta aaaactgttg acacaaaaca
    12481 gtttttaatt gtagattgca ctcaccaacc cactccgccc cacagaacgc caggcaatac
    12541 ttcgagttac ccggcagtgg tcaggggtta agtagccagc ccgaggcgta tgaacatgac
    12601 ggcgggaaca ctttgtataa cagcgcagca ggtttttagt tccgcgaccc ggcgttaagg
    12661 ggaaatgagg tcaacatgga tacgctcaat cttggcaaca acgaatctct ggtatgcggt
    12721 gtgttcccta accaggacgg cacgtttacc gcgatgacgt ataccaaaag taaaaagttt
    12781 aaaaccgaag ctggcgcgcg tcgctggtta gccagaaaca ctaactgatt agcaccagta
    12841 aaaacaggtt gccactggtt aatttaccct gaaaagtcag ggcacaacag aaagcacacg
    12901 gcgaaggcgt cttttcctta gaaggcttgt cgttagattt cttcgaccgt gtgcttccgg
    12961 ttgtggcaat ccgcgaaatg gcgcggcggt aaatatggct gggggttttt atttcttttc
    13021 ccctcattga ggacaccggg ttgtcaggtt gaccatatgc ctgagtgaca accccgccac
    13081 aacatctcca tgtttcaatg gcagtggtac ctttggcggc atcagtttat tagctttctg
    13141 atgtccgccc tttttaaagt gaattttgtg atgcggtgaa tgcggctaag cgcacgcgga
    13201 acagttaaaa ccgtaaagtg gtcttttacg gggcgtaacg ggcatacttc tgtattccgg
    13261 cgttaattgt taactggtta acgtcacctg gaggcaccag gcactgcatc gacaaagttc
    13321 acttcggtga tgaaaggtaa gagaaaatgt tgaatgtagc tattgaaaac cagaacgggt
    13381 ggaattatag tgcacctgca cctcataaag cgggtgccgg gattgctacc ccgatgttca
    13441 tcatggcgca taaccgcgct caggcggttt ttttatgcgt aatgcacagc cacattcaga
    13501 ttatggtggg gcgtgcaggg cagccgcaag gctggccggg ttccatgatg accggttgta
    13561 gcaaccctgt gcgtctcacc acccatgaga ttgctacctc cggtggtgag ttaatgaaat
    13621 tcatcatgga ggctgccatc atggctacta tccctgccct ttctcaccct gaaattacta
    13681 tcgccaatgg gcgtgctgtt acatcctctt tagctgttgc cgagtatttc cggaaaccac
    13741 acaaagatgt actggcaaaa atttcacgtc tagactgttc agcagaattc actgagcgaa
    13801 atttttcgcc cagtgaatat accgactaaa ccgggcgcaa actccctatg taccaaatca
    13861 ccaaaaacgg cttcgttttc ctggtgatgg gcttcaccgg caaaaaagcc gctgcattta
    13921 aagaagccta catcgctgag ttcgatcgca tggagaaaga actgcgccag aataacgccc
    13981 cgtctcccga caaaatgatt cacggggacg gacgtaccct ggttatccgt ctcgacgaac
    14041 acggcaatat caaattcact gaaaccgttc cggacggcgc aatggtctgc accctggata
    14101 ccttccagtt ttatctggag aaacaaggct ggactcttgt aaaccggagc gcaattaaaa
    14161 atatgactgt ggagcaatta ctaaaaattc attgttgagg acgcgataat ggaaacgtta
    14221 ttaccaaacg ttaatacgtc tgaagggtgt tttgatattg gtgttctgct cagtaaccgg
    14281 gagtttactg aagatgccat taagatgaga aaatatgagc cttatcttct caatgataat
    14341 tccatactct ccagaattgc ccttcttaaa cttggtattt tcggagggca gcagtgactt
    14401 ctgcatttgc actggtgatg acggtttttc ttataacggg tgagccacag aatgtgatta
    14461 ccggaattta tgccagtaaa gaatcatgcc tccgtgcaag agacgagcaa aaaatttctg
    14521 gcgaatgcct cccgttaaaa aaagtatcgc tgtacctgaa taacgaaata ccggctggat
    14581 aatccaccag ccatattaac gccatacccg ttgattaagc atgccagcaa tggcaggggt
    14641 tcgtacaacc ttaaaatagt tatgaggttt atccatgagc actgataaag aagaattttc
    14701 gctatattgc gaagcaaaaa atgacaaggt cagaaaacgc cttgggatta aaggaggttt
    14761 ttactggact acagcaaaaa aattatctgt tgccatctca cggtgcgttg ttgcaatgga
    14821 cgaggcaggc tacgacgctg atgatttcaa aaaacctgtc cgcgtccatt tccccgttgt
    14881 gaatgacctt ccaccggaag gcgtgtttga taccgaattc tgcaaccgct atgaaaaagg
    14941 cggggaagat ggcatcacaa tgatatttat agcgccttcc ccctcagttc aggacaaacc
    15001 agccagcact gacaatacca acgtcaatgg cgaagacatg gctgagattg aggataatat
    15061 gctcctgccg atttccggtc aggaactgcc cattcgctgg cttgcgcaac atggcagcga
    15121 aaaaccggta acgcacgttt cacgggaaga acttcaggca ttacatatcg cacgagctga
    15181 agaactgccg gctgttactg ccctggctat ttcccacaac acaaagctgc tcgacccgct
    15241 ggagattcgc gaccttcaca aactggtacg cgacacagac aaagttttcc ctaatcccgt
    15301 taattccagt ctggggttaa tgactgcttt tttcgaagca tacctggacg ctgactatac
    15361 cgatcgaggt ctgctgacaa aagagtggat gaaaggaaat cgtgttttac gcatcagccg
    15421 cacgccatcc ggcgctaatg ctggcggagg aattcttacc gatcgcggtg aaggttttgt
    15481 ccacgatgat gcgtcagtgg aacgtgacgt tgccgctggc gttctggccc gttcaatgga
    15541 catcgatatt tacaatccac atccggcaca cgccaaacgc attgaagaaa tcgtttcaga
    15601 gaataagccg cccttttctg tttttcgtga caaattcatc gccatgcctg gtcacctgga
    15661 ttattcccgc gcgatagtgg ttgcgtccgt gaaagaagca ccaattggta tcgaggctac
    15721 tccccaccgt gttaccgaat atctgaacaa agtactgacc gaaaccgacc atgccaaccc
    15781 tgatccagaa atcgtggata ttgcctgcgg tcgctcctct gctccaatgc cgcagcgtgt
    15841 aacaaaagaa ggaaaacagg atgatgaaga aaaaccgcag ccatctggcg caatggcaga
    15901 tgaacaggca acgactgaag cagtggaacc ggatacaact gaacataatc aggacacgca
    15961 gtcgatggat gctcagccac agataaattc tgttgatgcg aaatatcaga aactgcgtgc
    16021 agaactctat gaagccagga aaaacattcc acccaaaaat cctgtcgatg cagataaatt
    16081 actggctgct tctcacggag aatttgttga agggattagc gacccgaatg atccaaaatg
    16141 ggtgaagggg attgaaaccc gcgattctgt gaaccataac cagcaagaaa cggaacagaa
    16201 aggccataaa gcggaacaac acagtccaaa tgcgcaacaa aacgagccag aaacgaaaca
    16261 gcctgaacca gtagcgcaac aggaaccgga aaaagtctgc acagcctgcg gtcagaccgg
    16321 cggcggcaac tgtcctgact gtggcgcggt gatgggcgac gcaacatacc aggagacatt
    16381 caacgaagaa agccaggatg aagcccggga aaaagatccg gaggaaatgg aaagtgccgg
    16441 actcccgaac aaggagtgca ccgaaggcga tcaacatgcc aatggcaata atgaaacagg
    16501 cgagacagca aatcccttaa ttaaggtgaa cggtcatcgt gaaatcacat ccaccagcag
    16561 gttgtggcac catctgatga ttgaccttga aacaatgggc aaaaatcctg atgcgccaat
    16621 agcctcaata ggcgctgtat ttttcgatcc acaaaccgga gagcaggggc ctgaattcag
    16681 caaaataatt gatatgggta catgtggcgg cactgtagac ataagcacca tcgaatggtg
    16741 gcttcaacgc tctggcgaag cccgtgccgc cattttagct gatcgaatac cgcttgatga
    16801 tgcgctttta caattacggg aatttataga cgaaaactcc ggtgagtttt ttgttcaggt
    16861 ctggggaaat ggagccaact tcgacaacgt gattttacgc cgttcatatg aacggcagga
    16921 gatcccctgc ccgtggcgtt acaccaatga tcgcgatgta agaacgatgg tcgctctggg
    16981 gttggtgatg gatttcgatg cccgcaacgt caccacattt gagggtgaac gccataatgc
    17041 cctgcacgat gcgcgttacc aggcaaaata cgtttcagct atctggcaaa aactgttccc
    17101 aaatcaggct gatttttaat gttcaaccct gatcgccgct aaccgcatat agttagcggc
    17161 ggttatgaga tatagctatg agcagcttat ttttaaccga agatgaattg ctaatattaa
    17221 cgggctgcaa atatgcaagc caccagcgca actggttaat aaaaaatggg cttccgttct
    17281 ataccaatcg tagtggaaaa ccgatcgtca gtcgggaact gtttacctgt agaaacactt
    17341 taccaccacg tgaggctgaa cctgatttcg gtgcgatcta atgggaagac gaaggaaaaa
    17401 tcccgaacac gaaaaattac cgccaaaggt atatcccaat aaatatagtt atgtatggaa
    17461 accaacatcc agagaatctg taaccttaac tgcaatcaag gatggtttag ccgcattatg
    17521 gaaaaaatat gaagaaacgg ttaaccatca tgatcacgca atgacatttg ggcgtttgtg
    17581 ggaaaaattt ctcgccagcg cctattacag cgatcttagt cccagaacgc aaaaagatta
    17641 tctgcaacat caaaaaaaac tgctggccgt attcggtaag gtgctggccg attctgtaaa
    17701 accagagcac atcagacgat acatggacaa aaggggcgag cagagtaaaa cgcaggcaaa
    17761 ccatgaaaaa agcagcatgt cacgcgttta cagttggggg tatgaacgag ggtatgtaaa
    17821 ggctaatcct tgtgccggtg taagtaaatt caaggccaaa aaccgagagc gctatgtgac
    17881 tgacaaagaa taccaggccg tattaagcgt ggctcctgtg cctgttttta tcgcaatgga
    17941 aattgcatac ctgtgcgcag caaggatttc cgatgtgttg tctctgaaat gggaacagat
    18001 cggaaacgac gggatcttta tccagcaagg taaaacagga aagaaacaga taaaagcctg
    18061 gactccccgt ctgcaatcag ttatagaaaa agctaaacag ctacccaagt cagcctatgt
    18121 aatcagcaat cagtacggca accgttacat gtataaagga tttaatgaaa tgtgggttga
    18181 ggcaagaaat ttggctggac aaatatcagg gatcgcgacg gattttacat tccatgacct
    18241 taaagccaag gggatatcag attacgaagg tagtagccgg gataagcaac ttttctctgg
    18301 tcacaagacc gaaggacagg tgctaatcta tgatagaaaa gtaaagatct cacccacact
    18361 ggatgtacca ttaccccaga atattccaac aaaatattcc aagtaattcc aagtgtgatt
    18421 ttcatcattg acttaatgat gtgtaagtga ttgaattctg gcggagagag ggggatttga
    18481 acccccggtg gagttgcccc cactccggtt ttcgagaccg gtccgttcag ccgctccggc
    18541 atctctccgt tcagatggtt gccatgatgc caggaaattt ggcattttaa cagtccctgt
    18601 ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac gatgattaag tggccctgga
    18661 aagtacaaga atcagcacat caaactgccc ttccctggca ggaagcacta tcgatccccc
    18721 ttttaacgtg tctgacggaa caggaacaaa gcaaattagt cactcttgcc gaacgttttt
    18781 tacagcaaaa gcggcttgtt cctttacagg gctttgaact ggattcatta agaagctgcc
    18841 ggatagcact tctattttgc ctacccgttc tggagttagg actggaatgg ctggatggtt
    18901 ttcatgaagt cttaatttat cctgcgccat ttgtggtcga tgatgaatgg gaagacgata
    18961 tcggtctggt gcataaccaa cgtattgttc agtcaggtca gagctggcag caagggccta
    19021 tcgttttgaa ctggttggat atacaagatt cttttgatgc ttctggtttt aacctgatta
    19081 ttcatgaagt cgctcataag ctggacaccc gtaacggcga tcgcgccagc ggagttccct
    19141 ttattccgtt gcgtgaggtt gctggctggg aacacgatct tcatgctgca atgaacaaca
    19201 ttcaggaaga aatcgaattg gttggcgaga atgcggcgag cattgatgct tatgctgcca
    19261 gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt ctttagcgcc ccagaacttt
    19321 ttgctcctcg tttcccttca ttgtggcaac gtttctgcca attttatcaa caagatcctt
    19381 tgcagagact gcatcgcact aatgatacag actcgttttc ggcgacgaat gttcattaat
    19441 taacaacttt gcagattaat taaccaattg aaatgactta tgaaatttag tgttgacaga
    19501 caaggtaccg ctaagtaata ttcgccccgt tcacacgatt cctctgtagt tcagtcggta
    19561 gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc
    19621 taaaaattcg cttcttagcg caatgtcact aaccttagtt gaacattgtt tattaacgga
    19681 tagcgggttt ttaacatctt aagcgccctc gacctttatg gttgagggcg ttttgctatg
    19741 aacgccatca ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct
    19801 tgcaactcac acccaaaact gcctaacgaa aagttattaa ttttcaatca tattgctatc
    19861 aggatttaca ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa
    19921 ggtaagcatg gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatcttatg
    19981 cgggatggga attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc
    20041 gttccctatg gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc
    20101 tgcaccgtat tgccattgca aatgccaata cggtgcccta cacccttgga gcgctggaat
    20161 cggcccaaag cgttgccgaa cgtttcggta tttcggtggc tgagttacgc aaactcaacc
    20221 aatttcgtac gtttgctcga ggttttgata atgtccgcca gggtgatgaa ctggatgtcc
    20281 cggcacaagt tagtgaaaat aatttaaccc cgccaccggg taatagcagt ggcaaccttg
    20341 agcaacagat agccagtact tcacagcaaa tcgggtcttt gctcgccgag gatatgaaca
    20401 gcgaacaagc ggcaaatatg gcgcgtggat gggcctcttc tcaggcttca ggcgcaatga
    20461 cagactggtt aagccgcttc ggtaccgcaa gaatcacgct gggcgtggat gaagatttta
    20521 gcctgaagaa ctcccagttc gattttctcc atccgtggta tgaaacgcct gataatctct
    20581 ttttcagtca gcatactctc catcgtactg acgagcgtac gcagattaac aacggcttgg
    20641 gttggcgtca tttcactccc acatggatgt cgggcatcaa cttctttttc gaccacgatc
    20701 ttagccgtta ccactcccgc gccggcatta gcgcggagta ctggcgcgac tatctaaaat
    20761 taagcagtaa cggctatttg cgactgacca actggcgcag cgcacctgag ctggacaacg
    20821 attatgaagc ccgcccggcc aatggctggg atgtacgcgc agaaggctgg ttacccgcct
    20881 ggccgcacct tggcggtaaa ctggtctatg aacagtatta tggcgatgaa gtggccctgt
    20941 tcgataaaga cgatcggcaa agtaatcctc atgccataac cgctggactt aactataccc
    21001 ccttcccgct gatgaccttc agcgcggagc aacgccaggg taaacagggc gaaaatgaca
    21061 cccgttttgc cgtcgatttt acctggctac ctggcagcgc aatgcagaaa cagcttgacc
    21121 cgaatgaagt cgctgcacgg cgtagccttg caggcagccg ttatgatctg gtggatcgca
    21181 acaacaatat cgttctggaa tatcgcaaaa aagaactggt tcgcctgacc ctgacagacc
    21241 ccgtgacagg gaagtcagga gaagtgaaat cactggtttc gtcgctacaa accaaatatg
    21301 ccctgaaagg ctataacgtc gaagccaccg ctctggaagc tgccggtggt aaagtggtta
    21361 caacgggtaa agatattctg gttaccctgc cggcgtaccg gttcaccagt acgccagaaa
    21421 ccgataacac ctggccgatt gaagtcaccg ctgaagatgt caaaggcaat ttgtcgaatc
    21481 gtgaacagag catggtggtc gttcaggcac ctacgctaag ccagaaagat tcctcagtat
    21541 cattaagtac ccaaacattg aacgcggatt cccattcaac cgccacacta acttttattg
    21601 cgcatgatgc agcaggtaat cctgttatcg ggctggtgct ttcgacgcgt cacgaaggtg
    21661 ttcaggacat caccctgtcg gactggaaag ataatggtga cggaagctat acccaagtcc
    21721 tgaccacagg agcgatgtct ggcacgctga cgcttatgcc acagctgaac ggtgtggatg
    21781 cggctaaagc ccccgccgtg gtgaatatca tttctgtttc gtcatcccga actcactcgt
    21841 caattaagat tgataaggac cgttatctct ccggcaatcc tatcgaggtg acggtagaac
    21901 tgagagatga aaatgacaga cctgttaagg agcaaaaaca gcaactgaat acc
//
LOCUS       DAZFVO020000082        21880 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFVO020000082 DAZFVO020000000
VERSION     DAZFVO020000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.082"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            317..988
                     /locus_tag="ACHKNB_RS14250"
                     /old_locus_tag="pgaptmp_002850"
     CDS             317..988
                     /locus_tag="ACHKNB_RS14250"
                     /old_locus_tag="pgaptmp_002850"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HGU4196328.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            998..2194
                     /gene="yaiP"
                     /locus_tag="ACHKNB_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /pseudo
     CDS             998..2194
                     /gene="yaiP"
                     /locus_tag="ACHKNB_RS14255"
                     /old_locus_tag="pgaptmp_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2172..2753
                     /locus_tag="ACHKNB_RS14260"
                     /old_locus_tag="pgaptmp_002852"
     CDS             2172..2753
                     /locus_tag="ACHKNB_RS14260"
                     /old_locus_tag="pgaptmp_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4196329.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIESEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2755..3528
                     /gene="yaiO"
                     /locus_tag="ACHKNB_RS14265"
                     /old_locus_tag="pgaptmp_002853"
     CDS             2755..3528
                     /gene="yaiO"
                     /locus_tag="ACHKNB_RS14265"
                     /old_locus_tag="pgaptmp_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HGU4196330.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3713..3988
                     /gene="frmR"
                     /locus_tag="ACHKNB_RS14270"
                     /old_locus_tag="pgaptmp_002854"
     CDS             3713..3988
                     /gene="frmR"
                     /locus_tag="ACHKNB_RS14270"
                     /old_locus_tag="pgaptmp_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HGU4196331.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4023..5132
                     /gene="frmA"
                     /locus_tag="ACHKNB_RS14275"
                     /old_locus_tag="pgaptmp_002855"
     CDS             4023..5132
                     /gene="frmA"
                     /locus_tag="ACHKNB_RS14275"
                     /old_locus_tag="pgaptmp_002855"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HGU4196332.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5225..6058
                     /gene="fghA"
                     /locus_tag="ACHKNB_RS14280"
                     /old_locus_tag="pgaptmp_002856"
     CDS             5225..6058
                     /gene="fghA"
                     /locus_tag="ACHKNB_RS14280"
                     /old_locus_tag="pgaptmp_002856"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4196333.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6183..6722)
                     /gene="yaiL"
                     /locus_tag="ACHKNB_RS14285"
                     /old_locus_tag="pgaptmp_002857"
     CDS             complement(6183..6722)
                     /gene="yaiL"
                     /locus_tag="ACHKNB_RS14285"
                     /old_locus_tag="pgaptmp_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HGU4196334.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6824..8035)
                     /gene="mhpT"
                     /locus_tag="ACHKNB_RS14290"
                     /old_locus_tag="pgaptmp_002858"
     CDS             complement(6824..8035)
                     /gene="mhpT"
                     /locus_tag="ACHKNB_RS14290"
                     /old_locus_tag="pgaptmp_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HGU4196335.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8310..9323)
                     /gene="mhpE"
                     /locus_tag="ACHKNB_RS14295"
                     /old_locus_tag="pgaptmp_002859"
     CDS             complement(8310..9323)
                     /gene="mhpE"
                     /locus_tag="ACHKNB_RS14295"
                     /old_locus_tag="pgaptmp_002859"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HGU4196336.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9320..10270)
                     /gene="mhpF"
                     /locus_tag="ACHKNB_RS14300"
                     /old_locus_tag="pgaptmp_002860"
     CDS             complement(9320..10270)
                     /gene="mhpF"
                     /locus_tag="ACHKNB_RS14300"
                     /old_locus_tag="pgaptmp_002860"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HGU4196337.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10267..11076)
                     /gene="mhpD"
                     /locus_tag="ACHKNB_RS14305"
                     /old_locus_tag="pgaptmp_002861"
     CDS             complement(10267..11076)
                     /gene="mhpD"
                     /locus_tag="ACHKNB_RS14305"
                     /old_locus_tag="pgaptmp_002861"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HGU4196338.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11086..11952)
                     /gene="mhpC"
                     /locus_tag="ACHKNB_RS14310"
                     /old_locus_tag="pgaptmp_002862"
     CDS             complement(11086..11952)
                     /gene="mhpC"
                     /locus_tag="ACHKNB_RS14310"
                     /old_locus_tag="pgaptmp_002862"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HGU4196339.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSLNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11970..12914)
                     /gene="mhpB"
                     /locus_tag="ACHKNB_RS14315"
                     /old_locus_tag="pgaptmp_002863"
     CDS             complement(11970..12914)
                     /gene="mhpB"
                     /locus_tag="ACHKNB_RS14315"
                     /old_locus_tag="pgaptmp_002863"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HGU4196340.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFSQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAVKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12916..14580)
                     /gene="mhpA"
                     /locus_tag="ACHKNB_RS14320"
                     /old_locus_tag="pgaptmp_002864"
     CDS             complement(12916..14580)
                     /gene="mhpA"
                     /locus_tag="ACHKNB_RS14320"
                     /old_locus_tag="pgaptmp_002864"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HGU4196341.1"
                     /translation="MAIQHPDIQPAVNHSVQVAISGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14771..15604
                     /gene="mhpR"
                     /locus_tag="ACHKNB_RS14325"
                     /old_locus_tag="pgaptmp_002865"
     CDS             14771..15604
                     /gene="mhpR"
                     /locus_tag="ACHKNB_RS14325"
                     /old_locus_tag="pgaptmp_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HGU4196342.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     H"
     gene            15681..16763
                     /gene="lacI"
                     /locus_tag="ACHKNB_RS14330"
                     /old_locus_tag="pgaptmp_002866"
     CDS             15681..16763
                     /gene="lacI"
                     /locus_tag="ACHKNB_RS14330"
                     /old_locus_tag="pgaptmp_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HGU4196343.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQVQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16886..19960
                     /gene="lacZ"
                     /locus_tag="ACHKNB_RS14335"
                     /old_locus_tag="pgaptmp_002867"
     CDS             16886..19960
                     /gene="lacZ"
                     /locus_tag="ACHKNB_RS14335"
                     /old_locus_tag="pgaptmp_002867"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGU4196344.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20012..21265
                     /gene="lacY"
                     /locus_tag="ACHKNB_RS14340"
                     /old_locus_tag="pgaptmp_002868"
     CDS             20012..21265
                     /gene="lacY"
                     /locus_tag="ACHKNB_RS14340"
                     /old_locus_tag="pgaptmp_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HGU4196345.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21331..>21880
                     /gene="lacA"
                     /locus_tag="ACHKNB_RS14345"
                     /old_locus_tag="pgaptmp_002869"
     CDS             21331..>21880
                     /gene="lacA"
                     /locus_tag="ACHKNB_RS14345"
                     /old_locus_tag="pgaptmp_002869"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HGU4196346.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIR"
BASE COUNT         5174 a         5891 c         5624 g         5191 t
ORIGIN      
        1 atattacatt cacataatgt atataaacaa tacttgttca cagaacatat tttttaaatg
       61 caattaattg acaaactaaa tcacatctaa atacaatgta taaaacaaaa taactataca
      121 taccaaacat acaaacactt aacatatgaa ttgcataaaa acattaccca gtcgaacctc
      181 cgggtctgaa agcccttgct tttcagttat tagcaacatt taacatttat aaattaacat
      241 ctggctgcgc cagagattac gtctctgagt gcatcgttgt gcttgtagta taataaataa
      301 aatggagtgt cattaagtgg ataaggtttt agattcagcc ctcctttctt cggcaaataa
      361 aagaaagggt atccttgcta ttggcgcaca tcctgacgat atagaattag gctgtggcgc
      421 atcgcttgct cgtcttgcgc aaaaaggaat ttatatcgca gcagtggtga tgactaccgg
      481 caactctggt acagatggaa taatcgatcg ccatgaagaa tcgcgcaacg ccctaaagat
      541 attagggtgc caccaaacta ttcatcttaa ttttgctgac acccgcgctc atttacagct
      601 caatgatatg atttccgccc tggaagacat cattaaaaat caaattcctt ctgatgttga
      661 aatcatgcgg gtatatacca tgcatgatgc cgaccgccat caggatcatc tcgctgttta
      721 tcaagcttca atggttgcct gccgcactat tccacaaatt ctcggctacg aaaccccaag
      781 tacctggctt tcatttatgc ctcaggtttt tgaatccgtt aaagaagaat atttcacagt
      841 caaacttgct gcattaaaaa aacataaaag ccaggaacga cgtgattata tgcgtcatga
      901 tcgcctgcgt gcagttgcac aatttcgcgg gcaacaggtc aatagcgatc tgggtgaagg
      961 ctttgtaatt cataaaatga ttctttgagc aaattgcatg aaaacctgga tatttatctg
     1021 tatgtccata gcaatgttgc tatagttttt aagtacgcta agacgtaaac ccagtcaaaa
     1081 gaaaggctgt attgacgcca ttatacctgc gtataactaa ggcccgtgtc tggcgcagtc
     1141 actggataat ctactgcgta acccttactt ttgccgggta atttgcgtta acgacggctc
     1201 cacggacaat accgaagcgg tcatggcgga agtcaaacgc aaatggggcg accgctttgt
     1261 tgccgtcacg caaaaaaata ccggtaaagg tggtgcgctg atgaatggcc tcaattatgc
     1321 cacctgcgac caggtttttt taagtgatgc cgacacctat gttccgcccg atcaagacgg
     1381 aatgggctat atgctggcag aaattgagcg cggtgctgat gccgtaggcg gcattccctc
     1441 tactgcgttg aaaggcgcgg ggctgttacc gcacatccgc gcgaccgtaa agttgccgat
     1501 gattgttatg aagcgcacgc tacagcagct cctgggcggc gcaccgttta ttatcagcgg
     1561 tgcctgcggg atgttccgta ctgatgtatt gcgtaagttc ggtttctcgg atcgtactaa
     1621 agtcgaagac cttgatctca cctggacatt agtggcaaac ggctaccgta ttcggcaggc
     1681 gaatcgctgc atcgtatacc cacaggaatg caacagtccg cgtgaggagt ggcgtcgctg
     1741 gcggcgttgg attgtgggat acgcggtctg tatgcgcctg cataaaagac ttttatttag
     1801 ccgcttcggt atcttcagta tatttcctat gctgttggtt gtgatttatg gcgttgggat
     1861 ttatctcact acctggttta atgaattcat caccaccggg ccgcatggtg tggtgttggc
     1921 aatgtttccg cttatctgga tcggcgtagt ttgtgttatt ggtgctttta gcgcctggtt
     1981 tcatcgttgc tggttgttgg tgcctttagc gccgctttcc gttgtgtatg tattattagc
     2041 ttatgccatc tggattattt atggacttat tgcctttttt actggacgcg aacctcagcg
     2101 cgacaaaccc acccgctatt ccgcactggt ggaagcgtca accgcttatt cccaaccttc
     2161 tgtcacagga actgaaaaac tatctgaagc ttaatgttaa agagaaaaat attcagattg
     2221 cagaccaggt aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta
     2281 tttgtcatgg tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta
     2341 attatgcgtt tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca
     2401 ccgaaattaa aaatgcggtt attgaatcgg aagcaacgat tggtccgcaa tgttttattg
     2461 ccgactcggt agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc
     2521 gtctggatga acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg
     2581 ataaattagg ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt ataattttgc
     2641 ctgggcgaat tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt
     2701 tacctgccgg aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata
     2761 aaacggacgt tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact
     2821 tccattaccg cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta
     2881 gcgtatgctg aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag
     2941 gggcgtcgtg attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg
     3001 tataaatgga ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg
     3061 gtctttgccc gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt
     3121 ttcacgaccg gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa
     3181 ggcggcgtat cactctatac tggcccggtg atcaccagct accgctatac ccattatgac
     3241 tccagcgatg caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc
     3301 ggcactggtt atacgcaact atggctaagc cgcggaacag gtgcttacac ctatgactgg
     3361 acgccagaaa cacgctacgg cagcatgaag agcgtcagtc tgcaacgtat tcaaccgctg
     3421 actgagcaac ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat
     3481 gattataacg gtctgcaact tgcagcccat ctgacctgga aattctgatt ccttctgccg
     3541 cccgctatcc ggggcggcct tccctgccga ttagcccccc ctttcctctt tgttttccga
     3601 ccacattcac cggataaatt ttattctcca gtgttatata ctataggggg gtatgcattg
     3661 acatatagca taccccccta tagtatattg cgtgcagata atgaggtgcg aaatgcccag
     3721 tactccggaa gagaagaaaa aggtccttac tcgagttcgt cgtattcgag ggcagattga
     3781 tgctctggaa cggtcgctgg agggtgatgc cgaatgccgt gccatactcc aacagatcgc
     3841 tgccgttcgg ggcgcggcta atgggctgat ggcagaagtg cttgaaagcc atatccggga
     3901 aacgtttgac cgaaatgact gctacagccg cgaagtcagc caatccgttg acgacactat
     3961 tgaactggtt cgagcctatc ttaaatagct gaatctatta ccatattgag gaagagcgag
     4021 agatgaaatc acgtgctgcc gttgcatttg ctcccggtaa accgctggaa atcgttgaaa
     4081 ttgacgttgc accaccgaaa aaaggtgaag tgctgattaa agtcacccat accggcgttt
     4141 gccataccga cgcatttacc ctctctgggg atgacccgga aggtgtattc ccggtggttc
     4201 tcggtcacga aggggccggc gttgtggttg aagtcggtga aggcgtaacc agcgtcaaac
     4261 ctggcgacca tgtgatcccg ctttacaccg cggagtgcgg cgagtgtgag ttctgtcgtt
     4321 ctggcaaaac taacctctgt gttgcggttc gcgaaaccca gggtaaaggc ttgatgccag
     4381 acggcaccac ccgtttttct tacaacgggc agccgcttta tcactacatg ggatgctcaa
     4441 cattcagtga atacaccgtg gtcgcggaag tgtctctggc caaaattaat ccagaagcaa
     4501 accatgaaca cgtctgcctg ctgggctgtg gcgtgaccac cggtattggc gcggtgcaca
     4561 acacagctaa agtccagcca ggtgattctg ttgccgtgtt tggtcttggc gcgattggtc
     4621 tggcagtggt tcagggcgcg cgtcaggcga aagcgggacg gattatcgct atcgatacca
     4681 acccgaagaa attcgatctg gcgcgtcgct tcggtgctac cgactgcatt aacccgaatg
     4741 actacgacaa accgattaaa gatgtcctgc tggatatcaa caaatggggt atcgaccata
     4801 cctttgaatg catcggtaac gtcaacgtga tgcgtgcggc gctggaaagt gcgcaccgcg
     4861 gctggggtca gtcggtgatc atcggggtgg caggttctgg tcaggaaatc tccacccgtc
     4921 cattccagtt ggtcactggt cgcgtatgga aaggttccgc gtttggcggc gtgaaaggtc
     4981 gttcccagtt accgggtatg gttgaagatg cgatgaaagg tgatatcgat ctggaaccgt
     5041 ttgtcacgca taccatgagc cttgatgaaa ttaatgacgc cttcgacctg atgcatgaag
     5101 gcaaatccat tcgaaccgta attcgttact gatttcccgc aggtataccc cgtccacttc
     5161 agacggggtt cttaatactc tccctgggca gccgtccggg ggattaaccg tgagataatg
     5221 actgatggaa ctcattgaaa aacatgccag ctttggcggc tggcaaaatg tgtatcggca
     5281 ttattcccaa tcactgaaat gtgaaatgaa tgtcggcgtc tatctcccac caaaagccgc
     5341 gaatgaaaaa ttgccggtgc tgtactggct ttcgggcctg acctgcaacg agcagaattt
     5401 cattactaaa tcggggatgc agcgttacgc ggctgaacac aacattattg ttgttgcgcc
     5461 ggacaccagt ccgcgaggca gtcatgtcgc agatgccgac cgttacgatc tcgggcaagg
     5521 tgccgggttt tacctgaacg caacgcaagc gccgtggaat gaacattaca aaatgtatga
     5581 ctatatccgc aacgagctgc cggatttagt gatgcaacat tttccggcaa cgaccagaaa
     5641 gtctatctct ggtcattcta tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc
     5701 agatgaatat gtcagcgtct cggcgttttc gcccattgtc tccccatcgc aagtaccgtg
     5761 gggacagcaa gcctttgccg catatcttgg tgaaaataaa gatgcctggt tgaattacga
     5821 tccggtgagt cttatttcac aaggtcaacg cgttgcggaa atcatggttg atcaggggtt
     5881 gagtgatgat ttttacgcgg aacagctgcg gactccaaat cttgaaaaga tctgccagga
     5941 gatgaatatc aagacgttaa tccgttatca cgagggttac gaccacagct attattttgt
     6001 ctccagtttt attggcgagc atattgccta ccacgccaat aaactgaata tgcgttgata
     6061 atagtgcacg actgccggat gcggcgtgaa cgccttatcc gcctacattt cgcccgcagg
     6121 ccgtaggcat gataagacgc gcatagcgtc gcatcaggca tctgcgcacg acttatctat
     6181 gtttaccaca tcaaatcatc aggcactttg aagtcggcat atgggtcgtc ttcatcctgc
     6241 tcttcggcac tgagcgcgct gtgcaacaca atactgctgg catcacgctg agcaatttta
     6301 tcggcaacgc tggcgggaat aatggcgtat tcaccttcgc tattgttatc gaccaacaag
     6361 cgggcaatcg ccagacgacc attaatcaac tgcgcctgtg tgagcttatc gacaaaaatc
     6421 ttcttaatca gattgccgtc agtgaagtta aaaccaatat caccattggc aatggtgatt
     6481 cggttcattt caataagctg cttcacctgg gctttatatt ctttcgccaa cgccgcttgt
     6541 ttttgctgtt cgctaagctg tttatcacgc tcaagctgtg cctttttatt ttcttctacc
     6601 gccgcccgag cttcacgcgc ctgaacgcgc gattttttcg ccgttctctc gaccttcgcc
     6661 gcttttttgc tggtgactaa tcctgctttg agcaactgct cttgtaaggt aagttttgcc
     6721 atctttgttt ccaaacccgc tgaataattt ttagcgagta tacctgtaaa cgccgtgggt
     6781 gtattggggg caagtccctt tcgcccctgc taaataaggc gcgtcaggca tcggcacacg
     6841 gctgcattct tgatctccga ctcatcaaaa taaacaccgc caacccagca acaagaatac
     6901 ccggtgcaga agcggccatt acgccgaccg tgccagtgcc taatgccagc attttcccgg
     6961 ccagtaacgg accgctcata gcccccagac gccctaccgc cacggctgtt cccacacctg
     7021 ttgcgcggat ctgcgaactg taaaacaacg gtgccagggc atacaaaacg ctttgcccac
     7081 ctgtcgcaaa caaccccgcg acaaatcccg ccagcaacat accgttaaat gacgacaccg
     7141 ttccaagcgc cagcagcgaa gctaacatgc cgctataaat cagtagcgac atggttactg
     7201 gacgcagctt atccatcaat gcgcccaaca ttaacgtccc gcttgccgcc cccatttgca
     7261 gggcaaacat cacccctgcc gcctgcgatg gctggaatcc ttgctccacc aaaagtagcg
     7321 gtagccagtt gatcaacata taaaccacca gcagagtgaa gaaataacac aaccacagca
     7381 gcagcgtcgc ggttgccgtt tctggcgcaa ataaggcacg cagtggtggc gcagcctgtt
     7441 tttcgccagc gaaaaccgct gactccggca gccagcgcat taatagcggc accagaatca
     7501 acggcaccac accacctacc caaaacaccg tttgccatgc taagtttgcc cccgcgaaac
     7561 ccagtgtcgc cgccagcgcc gcgccaatgg gaacaccgca atacatcagg ctcactgccg
     7621 tcccacgaaa acgtggaccc gcggcttcag acgtcagggc gataagattc ggcaacgccg
     7681 cccccagccc gacaccggtc atcagccgcg caaagaccag tgaggggaaa tcccaggcaa
     7741 tcgccgttgc cagtgagaac aaaccaaaca gcgcaactga gccaatcaaa atactcttac
     7801 gaccataacg gtctgccagc atcccgccaa ccaacgcgcc gggtagcaaa ccgagtattc
     7861 cggcgctaaa tatccagccc atttgcattt tatcgagtgc gaaagcctgg gcgatgccac
     7921 ccgccgcaat gccagccgcc tgaagatcca gcccttccat cagagcgacc aaaaaacaaa
     7981 gcccgatggt cagcatcagg cgggatgaag atgatgaagg ggtacgagtc gacataagat
     8041 gaacctcgtt gcgagtgcag gtgaatcgcg actgatacat tgccagccgc gtaatcagaa
     8101 ctatttaatg actgcctacg ggcgaaatgt aggccggaca agacgtttat cgcatccggc
     8161 agtcgtgtgc agatgcctga tgcgacgcta acgcgtctta tcaggcctac aaattctcac
     8221 gccacccgta ggccggataa ggtgtttacg ccgcatccgg cagtcgtgct gggatgggca
     8281 tacaaaatgc acgttctcgg cagtcatcat tatttgttgt tgcgcagatc cagcgccacg
     8341 tcaacgatca tatcctcctg gccgccaacc atccggcgtt tgcccagctc aacgagaata
     8401 tccaccgcac ttaagccata acgcgccgcc gccgtttcac agtgacgcag gaagctcgag
     8461 taaacaccag cgtatcccag cgccagcgtt tcgcgatcga ctcgtaccgg tcgatcctgc
     8521 aacggacgca ccaggtcgtc agcggcatcc attaacgcat agagatcggt cccatgctgc
     8581 cagcccagtt tatccgcggc ggcaataaac acttccagcg gtgcgttacc tgcgcccgcg
     8641 cccattcccg cgaggctggc gtcgattcgg tcgcagccct cttccaccgc cgcgatagag
     8701 ttcgccacgc caagactcag gttatggtga gcgtgcatgc cagtttgcgt ttctggtttc
     8761 agctctgctt tcagggcgcg gaaacggtca cggatatcgc tcatgttcat cgcaccgcca
     8821 gaatccacca cataaataca ggtcgcaccg tagccttcca tcagctttgc ctgctttgcg
     8881 agattctccg gcgtggtcat atggctcatc atcagaaaac caacggtgtc cattccgagc
     8941 tcgcgggcat actgaatatg ctgggcggaa acatcagctt cggtacagtg cgttgccaca
     9001 cgaaccaccc gcgcgccagc ctgccaggca tttttcagat cgtgaatagt gccgattcct
     9061 ggcagcaaca acgtcgcgat tttggcgtgc ttcaccacat ccgccgctgc ttcaatccat
     9121 tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg aaccctgcaa accgtcgcca
     9181 tgggccactt caatcgaatc cacgcgggca tcgtccagtg ctttggcaat ctggcgaacg
     9241 ttttccagcg aatactgatg acgaatggcg tgcataccgt cacgcaacgt gacgtccgag
     9301 atataaagtt ttttaccgtt catgccgctt ctcctgcctt gcgcgccagt gactgggcca
     9361 ttttttccgc tgtcgccagc gcactggaag tcataatgtc gaggttgccc gcataggcag
     9421 gcagataatg cgctgcgcct tcgacttcca gccagaccgc tgttttcagt ccggagaatt
     9481 gccccacgcc cggtaaattg accggtttat cctgcgggat aacttcaaac tgcacgcgct
     9541 gtttcaggcg ataacccggt acgtaagcct gcaccgcctc agccatttca ttgattgagg
     9601 cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata caccgtgtca cgcatcatca
     9661 acggtggctc tgctgggtta agcacaataa tcgccttccc ttttgccgcg ccgcccacca
     9721 cttcaatggc tcgggaagtg gtttccgtaa attcatcgat attggcacgc gtgccaggtc
     9781 cggcagattt actggcgata gaagcgataa tttcggcgta atgaacacgc gccacgcgtg
     9841 aaactgccgc caccattgga atggtggcct ggccgccgca ggtgaccatg ttgacgttca
     9901 gttgatcgac gttcgcctcg aggttaacca ccggcacgca gtaagggccg atggcagcag
     9961 gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg taaagcggca tcgtttttca
    10021 catgcgcgcc cgcgctggtc gcatcaaata caatgtcgat atcagcaaat tcaggcatgt
    10081 tcatcagccc gatcacccct tcatgggtgg tggcgacgcc catacgtctg gcacgcgcca
    10141 gaccgtcgga ctgaggatca atgccgacca tcaccgccat ctccagatgc tgaccgtgac
    10201 gcaaaatttt aatcatcaga tcggtgccaa tgttgccaga accgataatg gcgactttac
    10261 gcttactcat gacagacttc cttttggggc tgcgcttgaa aatgtcgcag caactgaacc
    10321 tatgccttca atatgggctt caaaacgatc gcccgcattc actgccacca tcggacctaa
    10381 tgccccggta agaatgatat ctccggcgcg cagcggttca cccaggctgg ccattttgcg
    10441 tgccagccag acggcggcat taagcggatg tcccaggcat tcgctgccgc gcccgctgga
    10501 aacctcttcg ttattacgcg tcatcttcat ggcgcagttt ttcaggtcta accccgccgg
    10561 acgttgcgcc ggaccgccga tgacatacac cccgcaggaa gcgttatctg ccacggtatc
    10621 gacaaactga atcgaccagt cgcgaatgcg gctacccacc acctccagcg ccggaagtac
    10681 ccattcaatg gcgttataca gttcgtcgaa ggtgatatcg gttgcgggca aatcgcggtt
    10741 caacaccagt gcgatctccg cttcaatgcg ggcttgcaga acacgggaaa aaggaatgat
    10801 ttcgttatcg ccataacaca tgtcggcaaa taatgtccca aaatccggtt gatcaacgcc
    10861 cagttgttgc tgcactttcg gatgcgtcag gcccacttta cgccctacca cgcgacgccc
    10921 ctgcgcaacg tcatattgca catttatgtg ctgaatggcg taggccgctt cagcgttatc
    10981 gataccaatc agatcgcgca gcggcgcaat cgcttcgccc tgctctgcgg cgcggcgtaa
    11041 atccgccgcc agttgctcaa gagtatgctt cgtcatgacc attccttaag ggcgtgcgag
    11101 gaaattcagc accaattgat tgaaagcgtc ggcatgttcc cactgcgccc agtgaccaca
    11161 gtcgcggaag atatgcagtt cagaaccggc aatgccggac agcagacgca gacccgcatc
    11221 catcggcaca aagcggtcgt tgcgccccca gacaatcagg gtttgcgctt tgatttccgc
    11281 cagacgtggg ccaaaatccg ggaactgttt cggattagct tccaggctct taacgaagtt
    11341 ttccaggtga tcgcggcgcg acagcatatt attcaggcgc gcttcaaaca gggcgtcggt
    11401 caaatcgctg gtatcgaaaa cgaagatatc catcatcagc ttcaggtttt cgatagtcgg
    11461 ctgacgataa agctgattca gtcgcttaat accttcggtt ggcatcggcg taaacaaact
    11521 catgccgccc gtaccgccgc ccatcagcac cagtttgccg acgcgctccg gccagttaag
    11581 ggtgaacgcc atagaactat ggccgcccat cgagttgccc agcaggtgga ttttggcgat
    11641 atccagttga tccaccacgc ttttcaggat tcgtgcatta agatccgatc gtgaaccact
    11701 attaacgatc gaatcgcttt tgccccaacc cggacaatcc agcaggatta cccgatagcc
    11761 cgcctctacc agcggatcga tattgaggct gaagttcgcc cagccagtag cacccgggcc
    11821 ggaaccgtgc agcaggacaa cggtttcgtc gccttgtccg cagtcattaa aatggatgcg
    11881 cagcgtttta cccgcttctt ctacattcag aaaacggctg gtggcggctt cggtttgtgg
    11941 ctgataactc atcatcttct cctgcatatt cagttctctg ttctggcgct taacgagcca
    12001 aatccggcaa tccactccgg gattgggcgg taataacgcc cttcgctacg ccagttgcca
    12061 aatgcagaaa tagcggcaaa agcggcgacc caggttttga tttcatgtgt cgactttccg
    12121 gcaatggcgg aaagctcttc gttactgacg gcatccagtt cctgtatgcg tccctgctcc
    12181 agcaaagtca tgaactggtt atcccaaatc gggttgagcg gatgcagagt tctctgatcc
    12241 tcaacaaact tcacagcggc gctaatcacc cgttgctgac gcaattcgcg ctcactggcg
    12301 ggtaaatctt tcccgctccc caacagacgg tcgcgcatat gggcatcggc tttcgccagt
    12361 tcgggcaccg gcggctgatg ggaaagccca ccggaaccca ggaacagcac gcgtttattg
    12421 agagtgctgg tgaaacgtcc aatggcttca cccaacatgc gggtacgctg gaaaccgggc
    12481 agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg gcaccttatc cagcccaccg
    12541 agcaggaact ccagcggctg ggagaacccg tggtccacct gcatacagta agaaacggca
    12601 agatcgatcc cgctcttcat gacggcatgc gcacaggcct ccgccagctc cacaggcacg
    12661 ggcagctctc ctgccgcact gccgaaatca ccaattgccg tcgctccaac gcctaaacag
    12721 aacggtggca tcacgtcata gaaaaagccg ttgtagtgat ctggcgcaaa cagcaccacc
    12781 agttcagggg agaaagccgc aatacgctcg cgggcgctgg caatcacgcc attgacctca
    12841 tcgagtacct cttgtgccgg gtcgacatac cccaccagcg gcgagtggga aagacaatga
    12901 agataagcgt gcatatcagg ctaccttttc gacagaaacg tcggcatcag ggcgggtcag
    12961 cgtcatcacc gacgccagtt tattcagggt cttgcccagg gtttgcggaa tggcggtggc
    13021 ggcaacaaag cgatccgggc gcatcaccac cagcgaagca ttgtgttgtg cgaaccagct
    13081 acgcagacga ccttgcgtat cgcccacgcg tagcacgccg tcgtggttat cctgtgccgt
    13141 atgaatttgc acttccggca ccacctgaat aaagcgtgtg cccaacgcgc gccactgctg
    13201 gatttgctcg tcgctcatcc cccacagtgg attgcatccc cagccaatta ccgcgaagtt
    13261 cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt tccagcgtga ctttcggctg
    13321 aataaacatc ttgccgaccg gagagtgctt cgcttcgccc tcacgtacca gcgcaccgcc
    13381 gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag tagcgtttta ctggcggcag
    13441 ataattcagc agccaggaaa cgccgtcacg taacgtaccc tgccagcgtt tcggcggagc
    13501 cagcacgttg cccgccgtca cggacaggtc aatcatcgct ttggcgtgat cgcgacgttc
    13561 ttgttgatag gtattgagca gcgcatcgcg ggctttcccc tggataacca acgccagttt
    13621 ccaggcgagg ttaaaggcgt cgcgcatacc actgttatag ccctgcccct gccacaccgg
    13681 catgatgtgc gcggcatcgc ccgccagcag tacgcgatca atacggaaac gttgcgccag
    13741 tcgcgcgtta tgggtgtaga cacgctggcg aatcaattca acattgtccg gattaggcag
    13801 cactttgctt aacagcttgc gcatattttg cggctcacgc agctgctctt cggtttctcc
    13861 cggcatcacc ataaattcaa agcgacgtac cgcatgaggc agcgcggcag aaacatacgg
    13921 gcgcaccgga tcgcaacaca aatagatatg cggcgtactt aacggatcgt tggcgatatc
    13981 taccacaatc cactgatttg gcgcagtttt accttcaaac ggcacattca gggtgcgacg
    14041 aacaaaactt gccccaccat cacaggccac cagccactgg gctttgatcg tttcccgctg
    14101 cccttctgcc gttttcaggt gcaaggtcac ttcgtcatct tgctggctga aggcctccag
    14161 ctcgcgggaa aacaagcagc gcacattcgg aaaacgcgac aacccttcca gcatcaccgc
    14221 atcgacctgt ggctgaataa aggcgttacg gcgcggccag ccaaattcat cggtcattgg
    14281 ctgaatatca gcaaaacagc ggccttttgg ggtgagaaaa cgcatcgcgt gccacggtgt
    14341 agtgtgcggc agaacattct cgaccaggcc gaccgactgc atggtgcgca gcgcctcgtc
    14401 atcaatacca atcgcacgcg ggtagtcgat caacttatcg agtttctcca ccaccagcac
    14461 gtcaatgccc atctgaccga gatagttcgc catcatcagc ccgaccggac cggcaccaga
    14521 gatcgccacc tgaacgctat ggttaacagc aggctggatg tcagggtgtt gtattgccat
    14581 ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca cagtacggcg caacttattt
    14641 tgttaaaaac atgtaaatga ttttttattg tgcgctcagt ataggaaggg tgttttcggc
    14701 tacaatcaaa acatgcccga atgtgcacca ggtgcacaac gttgttttaa ctatagaaat
    14761 gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg gcttaacccg
    14821 cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg tcgggctgct
    14881 ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga cgctgcagga
    14941 agagggatat gtccgccgta gcccctccga tgacagtttt cgtctgacca tcaaagtgcg
    15001 gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg ccccgctgct
    15061 gggcgatctg ttgcgcgaag tggtatggcc aacagatgtg tccaccctgg atgttgatgc
    15121 aatggtggta cgcgaaacca cccaccgttt cagccgctta tcctttcacc gggcaatggt
    15181 cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg ccttttgccc
    15241 ggaacaagaa cgcaaggaat taatcgaaat gttagccgcc cgccccggtg atgactatca
    15301 actggcacga gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca aagagggtta
    15361 cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg ccgtaccgct
    15421 gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga gcgcaatgac
    15481 cattgaacag gccgcggaaa agcatcttcc ggcgctacaa cgggtagcaa aacagatcga
    15541 agaaggggtt gaatcgcagg ctattctggt ggccggacgg cgaagcggcg tgcatttaca
    15601 ttgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
    15661 gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca gagtatgccg
    15721 gagtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
    15781 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
    15841 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
    15901 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
    15961 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
    16021 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
    16081 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
    16141 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
    16201 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
    16261 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
    16321 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
    16381 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
    16441 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
    16501 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
    16561 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcaggtc caggcggtga
    16621 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
    16681 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaagttt
    16741 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagtcagct cactcattag
    16801 gcaccccagg ctttacactt tatgcgtccg gctcgtatgt tgtgtgaaat tgtgagcgaa
    16861 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtatt
    16921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    16981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    17041 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggttccgga
    17101 aagctggctg gagtgcgatc ttcctgtcgc cgatactgtc gtcgtcccct caaactggca
    17161 gatgcacggt tacgacgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
    17221 gccatttgtt cccacggaga atccgactgg ttgttactcg ctcacattta atgttgatga
    17281 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
    17341 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agccgtttgc cgtctgaatt
    17401 tgacctgagc acatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
    17461 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
    17521 cgtctcgttg ctgcacaaac cgagcacaca aatcagcgat ttccatgttg ccactcactt
    17581 taatgatgat ttcagccgcg cggtactgga agcagaagtt cagatgtatg gcgagctgcg
    17641 cgatgagctg cgggtgacgg tttctttgtg gcagggtgaa acgcaggtcg ccagcggcac
    17701 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
    17761 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
    17821 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcca gcgatgtcgg
    17881 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
    17941 tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
    18001 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
    18061 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtaga
    18121 tgaagccaac attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
    18181 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
    18241 gagtgtgatc atctggtcgc tgggaaatga gtcaggccac ggcgctaatc acgacgcgct
    18301 gtatcgctgg atcaaatctg tcgatccatc ccgcccggtg cagtatgaag gcggcggagc
    18361 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
    18421 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
    18481 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gcttcgctaa
    18541 atactggcag gcgtttcgtc agtacccccg tttacagggc ggcttcgtct gggactgggt
    18601 ggatcagtcg ctgattaaat atgatgaaaa tggcaatccg tggtcggctt acggcggtga
    18661 ttttggcgat acgccgaacg atcgccagtt ctgcatgaac ggtctggtct ttgccgaccg
    18721 cacgccgcat ccagcgctga cggaagcaaa acaccagcaa cagtttttcc agttccgttt
    18781 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
    18841 cctgcactgg atggtggcgc tggatggcaa gccgctggca agcggtgaag tgcctctgga
    18901 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
    18961 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
    19021 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
    19081 ctccgcgtcc catatcatcc cgcaactaac caccagcgaa acggattttt gcatcgagct
    19141 gggtaataag cgttggcaat ttaaccgtca gtcaggcctt ctttcacaga tgtggattgg
    19201 cgatgaaaaa caactgctga cgccgctgcg cgatcagttc acccgcgcgc cgctggataa
    19261 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
    19321 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
    19381 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
    19441 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
    19501 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
    19561 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
    19621 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
    19681 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
    19741 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
    19801 ccatcgccat ctgctgcacg cagaagaagg cacatggctg aatatcgacg gtttccatat
    19861 ggggattggt ggcgacgact cctggagccc gtcagtgtcg gcggaattcc agctgagcgc
    19921 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
    19981 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
    20041 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt
    20101 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
    20161 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
    20221 cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt
    20281 ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat cgattgttgg
    20341 tggtatttat ctaggctttt gttttaacgc cggtgcgcca gcagtagagg catttattga
    20401 gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg
    20461 ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc atcaataatc agtttgtttt
    20521 ctggctgggc tctggctgtg cactcatcct cgccgtttta ctctttttcg ccaaaacgga
    20581 tgcgccctct tctgccacgg ttgccaatgc ggtaggtgcc aaccattcgg catttagcct
    20641 taagctggca ctggaactgt tcagacagcc aaaactgtgg tttttgtcac tgtatgttat
    20701 tggcgtttcc tgcacctacg atgtttttga ccaacagttt gctaatttct ttacttcgtt
    20761 ctttgctacc ggtgaacagg gtacgcgggt atttggctac gtaacgacaa tgggcgaatt
    20821 acttaacgcc tcgattatgt tctttgcgcc actgatcatt aatcgcatcg gtgggaaaaa
    20881 cgccctgctg ctggctggca ctattatgtc tgtacgtatt attggctcat cgttcgccac
    20941 ctcagcgctg gaagtggtta ttctgaaaac gctgcatatg tttgaagtac cgttcctgct
    21001 ggtgggctgc tttaaatata ttaccagcca gtttgaagtg cgtttttcag cgacgattta
    21061 tctggtctgt ttctgcttct ttaagcaact ggcgatgatt tttatgtctg tactggcggg
    21121 caatatgtat gaaagcatcg gtttccaggg cgcttatctg gtgctgggtc tggtggcgct
    21181 gggcttcacc ttaatttccg tgttcacgct tagcggcccc ggcccgcttt ccctgctgcg
    21241 tcgtcaggtg aatgaagtcg cttaagcaat caatgtcgga tgcggcgcga gcgccttatc
    21301 cgaccaacat atcataacgg agtgatcgca ttgaacatgc caatgaccga aagaataaga
    21361 gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg aaaaaagact tcgtgggaaa
    21421 acgttaatgt atgagtttaa tcactcgcat ccatcagaag ttgaaaaaag agaaagcctg
    21481 attaaagaaa tgtttgccac ggtaggggaa aacgcctggg tagaaccgcc tgtctatttc
    21541 tcttacggtt ccaacatcca tataggccgc aatttttatg caaatttcaa tttaaccatt
    21601 gtcgatgact acacggtaac aatcggtgat aacgtactga ttgcacccaa cgttactctt
    21661 tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa acggcgagat gtactctttt
    21721 ccgataacga ttggcaataa cgtctggatc ggaagtcatg tggttattaa tccaggcgtc
    21781 accatcgggg ataattctgt tattggcgcg ggtagtatcg tcacaaaaga cattccacca
    21841 aacgtcgtgg cggctggcgt tccttgtcgg gttattcgcg
//
LOCUS       DAZFVO020000083        21795 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFVO020000083 DAZFVO020000000
VERSION     DAZFVO020000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.083"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..739
                     /locus_tag="ACHKNB_RS14350"
                     /old_locus_tag="pgaptmp_002870"
     CDS             38..739
                     /locus_tag="ACHKNB_RS14350"
                     /old_locus_tag="pgaptmp_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4196347.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            764..3364
                     /locus_tag="ACHKNB_RS14355"
                     /old_locus_tag="pgaptmp_002871"
     CDS             764..3364
                     /locus_tag="ACHKNB_RS14355"
                     /old_locus_tag="pgaptmp_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4196348.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     IWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSLGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            3355..4425
                     /locus_tag="ACHKNB_RS14360"
                     /old_locus_tag="pgaptmp_002872"
     CDS             3355..4425
                     /locus_tag="ACHKNB_RS14360"
                     /old_locus_tag="pgaptmp_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196349.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4437..4979
                     /locus_tag="ACHKNB_RS14365"
                     /old_locus_tag="pgaptmp_002873"
     CDS             4437..4979
                     /locus_tag="ACHKNB_RS14365"
                     /old_locus_tag="pgaptmp_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196350.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4987..5502
                     /locus_tag="ACHKNB_RS14370"
                     /old_locus_tag="pgaptmp_002874"
     CDS             4987..5502
                     /locus_tag="ACHKNB_RS14370"
                     /old_locus_tag="pgaptmp_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196351.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5468..6205
                     /locus_tag="ACHKNB_RS14375"
                     /old_locus_tag="pgaptmp_002875"
     CDS             5468..6205
                     /locus_tag="ACHKNB_RS14375"
                     /old_locus_tag="pgaptmp_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4196352.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6316..7326
                     /gene="pyrD"
                     /locus_tag="ACHKNB_RS14380"
                     /old_locus_tag="pgaptmp_002876"
     CDS             6316..7326
                     /gene="pyrD"
                     /locus_tag="ACHKNB_RS14380"
                     /old_locus_tag="pgaptmp_002876"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HGU4196353.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7500..8042
                     /gene="zapC"
                     /locus_tag="ACHKNB_RS14385"
                     /old_locus_tag="pgaptmp_002877"
     CDS             7500..8042
                     /gene="zapC"
                     /locus_tag="ACHKNB_RS14385"
                     /old_locus_tag="pgaptmp_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HGU4196354.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8039..9148)
                     /gene="ycbX"
                     /locus_tag="ACHKNB_RS14390"
                     /old_locus_tag="pgaptmp_002878"
     CDS             complement(8039..9148)
                     /gene="ycbX"
                     /locus_tag="ACHKNB_RS14390"
                     /old_locus_tag="pgaptmp_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HGU4196355.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9392..11500
                     /gene="rlmKL"
                     /locus_tag="ACHKNB_RS14395"
                     /old_locus_tag="pgaptmp_002879"
     CDS             9392..11500
                     /gene="rlmKL"
                     /locus_tag="ACHKNB_RS14395"
                     /old_locus_tag="pgaptmp_002879"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HGU4196356.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11512..13419
                     /locus_tag="ACHKNB_RS14400"
                     /old_locus_tag="pgaptmp_002880"
     CDS             11512..13419
                     /locus_tag="ACHKNB_RS14400"
                     /old_locus_tag="pgaptmp_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4196357.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13549..14802
                     /gene="pqiA"
                     /locus_tag="ACHKNB_RS14405"
                     /old_locus_tag="pgaptmp_002881"
     CDS             13549..14802
                     /gene="pqiA"
                     /locus_tag="ACHKNB_RS14405"
                     /old_locus_tag="pgaptmp_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HGU4196358.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14807..16447
                     /gene="pqiB"
                     /locus_tag="ACHKNB_RS14410"
                     /old_locus_tag="pgaptmp_002882"
     CDS             14807..16447
                     /gene="pqiB"
                     /locus_tag="ACHKNB_RS14410"
                     /old_locus_tag="pgaptmp_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HGU4196359.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16444..17007
                     /gene="pqiC"
                     /locus_tag="ACHKNB_RS14415"
                     /old_locus_tag="pgaptmp_002883"
     CDS             16444..17007
                     /gene="pqiC"
                     /locus_tag="ACHKNB_RS14415"
                     /old_locus_tag="pgaptmp_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HGU4196360.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17263..17430
                     /gene="rmf"
                     /locus_tag="ACHKNB_RS14420"
                     /old_locus_tag="pgaptmp_002884"
     CDS             17263..17430
                     /gene="rmf"
                     /locus_tag="ACHKNB_RS14420"
                     /old_locus_tag="pgaptmp_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HGU4196361.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17500..18018)
                     /gene="fabA"
                     /locus_tag="ACHKNB_RS14425"
                     /old_locus_tag="pgaptmp_002885"
     CDS             complement(17500..18018)
                     /gene="fabA"
                     /locus_tag="ACHKNB_RS14425"
                     /old_locus_tag="pgaptmp_002885"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HGU4196362.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18087..19847)
                     /locus_tag="ACHKNB_RS14430"
                     /old_locus_tag="pgaptmp_002886"
     CDS             complement(18087..19847)
                     /locus_tag="ACHKNB_RS14430"
                     /old_locus_tag="pgaptmp_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4196363.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20033..20485
                     /gene="matP"
                     /locus_tag="ACHKNB_RS14435"
                     /old_locus_tag="pgaptmp_002887"
     CDS             20033..20485
                     /gene="matP"
                     /locus_tag="ACHKNB_RS14435"
                     /old_locus_tag="pgaptmp_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HGU4196364.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20561..21601)
                     /gene="ompA"
                     /locus_tag="ACHKNB_RS14440"
                     /old_locus_tag="pgaptmp_002888"
     CDS             complement(20561..21601)
                     /gene="ompA"
                     /locus_tag="ACHKNB_RS14440"
                     /old_locus_tag="pgaptmp_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HGU4196365.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         5539 a         5144 c         5750 g         5362 t
ORIGIN      
        1 tggaggggat attcatattt tttaagagtg actatttatg aaaacttgca taacaaaggg
       61 aattgtgacc gtaagtttaa cggcaatatt actcagctgc tcatccgcat gggcggcagg
      121 taaaggcggg attggacttg cagcaacacg tcttgtttat tcagaaggtg aagagcaaat
      181 ttcactgggt gtacgtaaca ccagtccgga tgttccttat cttattcagt catgggtgat
      241 gaccccagat aataaaaaat cagcagattt tattattaca ccaccgctat ttgtgctgaa
      301 tccggctaat gagaatctgt tacgcattat gtacattggt gcgccgttgg cgaaagacag
      361 agaaaccctt ttcttcacta gcgtacgggc agtcccttca acaacgaagc gggaagaggg
      421 aaataccctg aagattgcca cacaaagcgt catcaaactt ttctggcgac caaaaggttt
      481 agcgtatccc ttaggcgagg ctccggcgaa actgcgttgc acttcgtcag ctgacatggt
      541 tacggtcagt aacccaacac cttatttcat taccctgaca gacctgaaaa taggtggaaa
      601 agtagttaaa aatcaaatga tttccccctt tgataaatac caattttctc tgccaaaggg
      661 ggccaaaaat agcagcgtaa cgtatcgaac catcaatgac tacggggcgg aaacgccgca
      721 actcaactgt aaatcgtaag ccgtcttcag ttaagagagc gagatgtata gaactcatcg
      781 acaacacagc ctgttaagct ctggtggagt gccatcgttt attggtgggc tggtggtgtt
      841 tgtgtcggca gcgttcaatg cacaagctga aacctggttc gatcctgctt ttttcaaaga
      901 tgatccctca atggtggccg atttgtctcg tttcgaaaaa ggacaaaaaa taacgccagg
      961 ggtttatcgt gtcgatattg ttctgaatca gacaattgta gatacgcgca acgtcaattt
     1021 tgttgagata acgccagaga aggggattgc cgcctgtttg acgactgaaa gcctggatgc
     1081 aatgggtgtt aatactgatg cgtttccggc ttttaaacaa ctggacaaac aagcgtgtgt
     1141 gccattggcg gagattattc cggatgccag cgtaacgttt aatgtgaata aactccgtct
     1201 ggaaatttca gtaccgcaaa tcgccatcaa aagtaatgcc cgtggttatg tcccccctga
     1261 acgttgggat gaagggatca acgcgctatt actgggatat tcgtttagcg gggctaacag
     1321 tattcatagc agcgcagaca gtgattctgg cgacagctat tttctgaatt taaacagtgg
     1381 cgttaattta ggcccatgga gattgcgcaa caattcaata tggagtcgca gtagtggcca
     1441 aaccgcagaa tggaagaatc tcagcagcta tttgcagcgg gcggttattc cactaaaagg
     1501 cgaactgacc gtaggtgatg attatactgc aggcgatttt ttcgatagtg tcagctttcg
     1561 tggtgtgcag ctggcgtcag atgacaacat gctgccagac agcctgaaag ggtttgcgcc
     1621 tgtggtgcgt ggtatcgcca aaagcaatgc ccagataacg attaagcaaa atggttacac
     1681 catttaccaa acttatgtat cgcctggtgc ttttgaaatt agtgatctct attccacgtc
     1741 gtcgagcggt gatttgttag ttgaaatcaa agaagcggac ggcagcgtca atagctacag
     1801 cgtaccgttt tccagcgtgc cattactcca gcgtcagggg cgaatcaaat acgcggtgac
     1861 actggcgaaa tacagaacca atagtaatga acagcaggag agcaaatttg cccaggccac
     1921 gttgcagtgg ggcggaccgt ggggaacgac atggtatggt ggtggacaat atgctgaata
     1981 ttaccgtgcc gccatgtttg gtctgggatt taaccttggc gatttcggag caatttcgtt
     2041 cgatgtgacc caggcgaaga gtacgctggc agaccaaagc gaacataaag gtcagtcata
     2101 tcgttttctg tatgccaaaa cgctcaacca attgggcact aattttcaat tgatgggcta
     2161 tcgctattcg acgtcgggtt tctacaccct ttccgacacc atgtataaac atatggatgg
     2221 ctacgaattt aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt attacaattt
     2281 gttttacacc aaacgtggca aactgcaggt caatatctcc cagcaattag gcgagtacgg
     2341 ttcgttttat ttaagtggta gccagcaaac ttactggcat accgatcaac aggatcggct
     2401 attacagttt ggctacaaca cgcaaattaa agatctctcg ctgggggttt cctggaacta
     2461 cagtaagtcc cgtggtcaac ctgatgctga tcaggtgttt gcactaaatt tttccctgcc
     2521 gctcaatctg ttgctcccca gaagtaatga tagctatacc aggaaaaaaa attacgcctg
     2581 gatgacctct aacaccagta tcgataacga agggcacatt acacaaaacc tgggtttaac
     2641 ggagacacta ctcgatgacg gtaacctgag ctacagcgtg caacagggat ataacagcga
     2701 ggggaaaacg gctaatggta gcgccagcat ggactacaaa ggggcgtttg cagatgcccg
     2761 agtgggctac aactacagcg ataacggcag tcaacaacaa ctgaactacg ctctttcagg
     2821 cagtttagtt gcccattcac tgggcattac cctggggcaa tcgctggggg aaactaacgt
     2881 cctgattgca gcaccaggcg cagagaatac tcgtgtggcg aacagcaccg ggctgaaaac
     2941 tgactggcgc ggatataccg ttgtccccta tgccacttct tatcgggaaa atcgaatcgc
     3001 acttgatgcg gcatcgttaa aacgtaacgt ggatcttgaa aatgcagtag tcaacgtggt
     3061 tcccaccaaa ggggcgttgg ttctggcgga gttcaatgcc catgcgggag caagggtatt
     3121 aatgaaaaca tcaaagcagg gtataccgct gcgttttggc gcgatagcga cgctggacgg
     3181 catacagact aatagcggca ttattgatga tgatggctcg ctctatatgt ccggtttgcc
     3241 cgctcaggga gcgataactg tacgctgggg cgaagctccc gatcaaattt gtcatatcag
     3301 ttaccagctt accgaacaac aaattaactc tgcgattacg cgaatggatg ccatatgcag
     3361 ataatctttg gagaaaaatg cgtgttatta ctacgactat tttttgccgc cgtcttaatg
     3421 ctatggtgcg ctcaaactgc tgcttatagc gggcagtgtc ataccactca ggggaatccg
     3481 tatattggcg tcaattttgg cgttaaaacc ctggaggaag aagaaaatac ggctggagta
     3541 gttaaagaca aattttatca gtggaacgaa tcgaatgatt attatgtttc ctgtgattgc
     3601 gataaagaca atgtcagaag tggccgatgg gcattcgccg cggattcacc gttagtctat
     3661 ttaggcgaca actggtacaa aattaatgac tatcttgccg ccaaagtttt attgcaggtt
     3721 aaaggcagtt ctcctactgc ggttcctttc gaaaacgtgg gcacaggggc agatacacga
     3781 tggcatattt gcgatcccgg cggtcaacgt ttaggtggcc agggggctag cggtaatagc
     3841 ggtagctttt ccctgaaaat attgcagccg ttcgttggtt cggtcgtcat tcctcctatg
     3901 gcgctggcgc gattatttga atgctacaac atacccgcag gtgattcctg cacgactaca
     3961 ggtacaccgg ttttagtgta ttacctgtct ggtactatca attcacttgg ctcatgttcc
     4021 gtcaatgccg gagaaacaat cgaggtcgat ctgggcgacg tatttgcggc taactttcgt
     4081 gttgtagggc ataagcctct tggggccaga acggcagaac ttgcaattcc agtcaggtgt
     4141 aacacgggaa acgcggggtt agttaacgtc aacctgagtc tgacggcaac cacagacccc
     4201 agctatcccc aggcgattaa gacgtcacgt cctggcgtgg gcgtggtggt gaccgatagc
     4261 cagaacaaca ttatttcccc tgctggtgga acattaccgc tctctattcc tgatgatgca
     4321 gacagtatcg cgcgaatgaa tgtctatcca gtcagcacga caggtgtacc accagaaacc
     4381 gggcgatttg aagccacggc aacggtgaga ataaattttg attaacgcgt gaacgtatga
     4441 agaaaaaaac gatatttcag tgcgttatct tgttctttag cattcttaac atccatgtcg
     4501 ggatggctgg gcctgaacaa gttagtatgc atatttatgg gaatgtggtc gatcagggct
     4561 gtgatgtcgc caccaaaagt gcattacaaa atattcatat tggtgatttt aatatcagtg
     4621 attttcaggc cgcgaatacc gtaagcactg ctgctgattt gaatatcgat atcaccggtt
     4681 gtgccgctgg tattactggc gcggacgtcc tttttagcgg cgaggctgac acccttgcgc
     4741 cgacactgct caaactaact gacacaggcg gaagcggtgg tatggcaacg gggattgccg
     4801 tgcaaattct tgatgcgcaa agtcagcaag aaatcccgct caatcaggtc cagcctctta
     4861 cgcccttaaa agccggggat aacacactca aatatcaact tcgttataag tccacaaagg
     4921 cgggagcaac gggcggtaat gcgacggcgg ttctctattt tgatctggtt taccagtgaa
     4981 ggagtgatgt tgaaacggat aatctggatt ctgttcttac tgggattaac ggggggctgt
     5041 gagctatttg cccatgatgg cacagtcaac attagcggat cgtttcgccg taatacatgc
     5101 gtgctggcac aggatagcaa gcaaatcaat gtgcagttag gcgatgtctc gctgacacgt
     5161 ttttttcatg ggaattatgg cccggagaaa tctttcatca ttaatttgca ggattgcgga
     5221 actgatgtca gcacggttga cgtcaccttt tcaggaaccc ccgacggagt gcagagcgaa
     5281 atgctgtcca ttgaaagtgg tacggatgca gccagcgggc tggcaattgc gattctggat
     5341 gatgcgaaga tattgattcc gcttaaccag gcgagtaaag actacagcct acatagcggt
     5401 aaggtgccat taacttttta tgcgcaattg cgacctgtca atagcgatgt gcagtcgggt
     5461 aaagtgaatg ccagcgcgac atttgtactt cattatgact aatacgtgga acagattggc
     5521 gctcttgatt ttcgccgttt tatcgctgct ggtggcgggt gaattgcagg caggagtggt
     5581 ggtcggcgga acgcgattta tctttccggc agacagagaa tcgatatcta ttttactgac
     5641 taataccagt caggaatcct ggcttattaa cagcaaaatc aaccgcccaa cgcgttgggc
     5701 cgggggggaa gcgtcgacag tgccagcacc attactggcc gctccgccac ttattctcct
     5761 gaagcccggt actacaggca cgttgcgctt gctgagaacg gaaagcgaca tcttgcctgt
     5821 ggatcgcgaa acgctatttg agttaagcat tgccagcgtg ccatccggca aagttgaaaa
     5881 tcagagcgta aaagtggcga tacgctcggt atttaaactg ttctggcgac ccgaaggttt
     5941 gccgggcgac ccgctggaag cttatcaaca attacgctgg acatggaatt cgcagggtgt
     6001 acaactcact aacccaacgc cttactacat taacctgatt caggtgagcg taaatggtaa
     6061 agcgttaagt aatgcgggag ttgtgccgcc taaaagccag cgtcagacaa gctggtgtca
     6121 ggcaatcgca ccgtgtcatg tcgcctggcg ggcgataaat gattacggcg ggttgagtgc
     6181 aaagaaggag caaaatctgc cctgaaacag gttcggaaaa cgtttgcgtt ttttttgccg
     6241 caggtcaatt cccttttggt ccgaactcgc acataatacg cccccggttt gcacaccggg
     6301 aatccaggag agttcatgta ctaccccttc gttcgtaaag cccttttcca gctcgatcca
     6361 gagcgcgctc atgagtttac ttttcagcaa ttacgccgta ttacaggaac gccgtttgaa
     6421 gcactggtgc ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct gacgtttaaa
     6481 aatccgcttg gtctggcagc cggtcttgat aaagacgggg agtgcattga cgcgttaggc
     6541 gcgatgggat ttggatcgat cgagatcggt accgtcacgc cacgtccaca gccaggtaat
     6601 gacaagccgc gtctctttcg tctggtagat gccgaaggtt tgatcaaccg tatgggcttt
     6661 aataatcttg gcgttgataa cctcgtagag aacgtaaaaa aggcccatta tgacggcgtc
     6721 ctgggtatta acatcggcaa aaataaagat acgccagtgg agcagggcaa agatgactat
     6781 ctgatttgta tggaaaaaat ctatgcctat gcgggatata tcgccatcaa tatttcatcg
     6841 ccgaataccc caggattacg cacactgcaa tatggtgaag cgctggatga tctcttaacc
     6901 gcgattaaaa ataagcaaaa tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca
     6961 gtgaagatcg cgccggatct ttctgaagaa gaattgatcc aggttgccga tagtttagtt
     7021 cgccataata ttgatggcgt tattgcaacc aataccacac tcgatcgttc tcttgttcag
     7081 ggaatgaaaa attgcgatca aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc
     7141 accgaaatta ttcgccgctt gtcactggaa ttaaacggtc gcttaccgat catcggtgtt
     7201 ggcggcatcg actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg
     7261 caaatttatt ctggttttat ttttaaaggt ccgccgctga ttaaagaaat cgttacccat
     7321 atctaattat ttcttcgaat tcctctttat aagataatca gggctttatt ttcagccctg
     7381 gttgttttat attcattgct gttgcttatt tagacatttt gtacttttat tgttgaggtt
     7441 attaagcgaa gcgacaatgg attgtgttgc tgcggtttta tagggtaggg gagaggcaga
     7501 tgcgaattaa accagacgat aactggcgtt ggtattacga tgaagagcac gatcgaatga
     7561 tgctcgattt agccaatggt atgctatttc gctcacgttt tgcgcgcaag atgttgactc
     7621 cagatgcctt ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat ttctcttttg
     7681 aagaaaagtg ccgcgatttt aatttatcta aagaacaaaa agccgaactg gtgctgaatg
     7741 cactggtagc aattcgctat ctcaaaccgc aaatgccgaa aagctggcat tttgtttccc
     7801 atggtgaaat gtgggtgccc atgccgggcg atgcggcctg cgtttggttg agcgatactc
     7861 acgagcaggt caatttgctg gtcgttgaat ctggcgaaaa tgccgcacta tgcctgctgg
     7921 cacaaccctg cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca
     7981 tgaacgacag gctgaaaccg caggttaatg ttgacagctt cagcctcgaa caggcagtct
     8041 aacgcgccaa cttaagtgca gtcttcggta cacagctaca gcaaagaatg gtgccatcat
     8101 cgcccattgc tgattttttc agcggcgtga cttcgccttc taaaagctga acacggcaac
     8161 ttccacaaat gcccgcgcgg caagaataag ggatacgaat tccctgattt tctaattgct
     8221 ccagcaacac ctgttggtta tttccacgaa atgcctgtcc ctgccagtca atatctacat
     8281 ttgcgtccgg ttgttgcgtg atgttggcgg tatcatcagc ggcagctgcg ccgtaaattt
     8341 ttgccggagc cgttgccaga atttccacct catcgccaac gcgaatcacg ccgctattac
     8401 gggcaattaa attctgacca aaatcgacat cgccgttatc ctgggcagtg cggaaagatt
     8461 gcaatgtttt taatggttcg cctgccggat gtttttgccc tttttctggg ctgacggtgg
     8521 tgaaaataca gcggctacaa ggtttaacca catcaaacac cacatcacca atgcgaatca
     8581 ctttccagcg atcttcttcc caggctgacg cgccggaaac caccagattg gggcggaatt
     8641 gctccatttt tacactggcc ggacaacgtt gttggagatc acgtaacgag gcttcgttag
     8701 caagaaggta aggatagcca tcagcaaatg acagaggtac agtgttgtgg cgtttcacgc
     8761 gccgggtcat ttgtggcccc acccagcgta attgcacttc gcgggagaaa aatccactta
     8821 gccatttgtt gatcgcgtct ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg
     8881 ttggtgcgtc ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg ccatctggtg
     8941 cggtgagatg caagccatca tgcacaggtg aaggagtaaa ccgtaccatc tggggaaact
     9001 ggcgagcggt aataaacgta ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca
     9061 gaccactgac atcagccaga gcatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat
     9121 gaataaaaag ccgggttaat gtcgccacgg tgcggtcctc aaatgaaaat aagccctcaa
     9181 ctttatgaca tgacgcgctt attagctata atgcgcaaca attttcttag cgactgtccc
     9241 attaggctat tttatttgtc agtttggcct tgggttgtgc tcgaaatcct cacgtacttg
     9301 tgtacgctcc ggtttctccg cgctgtccat gtccagactg actgcaacaa ttacgcctac
     9361 tgcggtaggc tcttagagta aaagtgacga tatgaattct ctgtttgcca gtacggcccg
     9421 tgggctggaa gagctgttaa aaactgaact ggaaaacctg ggggccgttg aatgccaggt
     9481 ggttcagggt ggggtccatt tcaagggcga cacacggctt gtttaccaga gcctgatgtg
     9541 gagccgcctg gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt acagcgattt
     9601 agacctctat ctcggtgttc aggcgatcaa ctggacagag atgtttaatc ctggcgcgac
     9661 cttcgctgtc cacttcagtg gtttgaatga caccatccgc aacagtcagt acggcgcgat
     9721 gaaagtgaaa gacgcgatcg tcgatgcttt cacgcggaaa aatctgccgc gtccaaatgt
     9781 tgatcgcgat gcgccggata tccgcgttaa cgtctggctg cataaagaaa ccgccagtat
     9841 cgctcttgat ctgagtggtg atggtttaca tctgcgtggc tatcgcgatc gtgctggtat
     9901 tgcgccgatc aaagaaaccc tggcagccgc tattgtgatg cgatccggct ggcagccagg
     9961 aacaccgctg ctcgatccga tgtgtggttc cggtacgttg ctgattgaag cggcgatgct
    10021 agcgaccgat cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg gctgggtgca
    10081 gcatgatgaa gatatctggc aggaagtgaa agcggaagcg caaactcgtg cccgtaaagg
    10141 cctggctgag tatagctctc atttttacgg ttcggacagc gacgcacggg tgattcaacg
    10201 tgcgcgcact aacgcccgtc ttgcggggat tggtgaactg atcacctttg aggtgaaaga
    10261 tgtcgcgcaa ctgaccaatc cgctgccgaa agggccgtac ggtacagtgt tgagcaaccc
    10321 gccatacggg gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata gcctgctggg
    10381 tcggatcatg aaaaaccagt ttggtggctg gaatctctct ttgtttagtg cctcgccgga
    10441 tctgctaagc tgcctgcagc tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct
    10501 ggactgcgta cagaaaaatt accacgttgc cgaaagcact ccagacagca aaccggcgat
    10561 ggtagcggaa gactacgcca accgcctgcg taagaacctc aaaaaattcg agaagtgggc
    10621 tcgtcaggaa ggaattgaat gttaccgcct gtatgacgcc gatctgccgg aatataacgt
    10681 tgccgttgac cgttatgccg actgggtggt ggtgcaggag tatgcaccgc caaaaactat
    10741 tgatgctcac aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca tttcggtact
    10801 ggggattgcg ccaaacaaac tggtgctgaa aacccgtgaa cgtcagaagg gcaaaaatca
    10861 gtaccagaaa ctgggcgaga agggcgagtt tctggaagtt accgaatata acgctcactt
    10921 gtgggtgaat ctgacggatt acctcgatac cggtctgttc ctcgatcacc gtatcgcccg
    10981 tcgtatgctc ggccagatga gcaaaggcaa agatttcctc aacctgttct cttataccgg
    11041 cagcgccacc gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg tggatatgtc
    11101 gcgtacttat ctggagtggg cagaacgcaa cctgcgtctg aatggcctga ccgggcgtgc
    11161 gcatcgcctg attcaggccg attgcctggc gtggctgcgt gaggcaaatg aacagttcga
    11221 tctgatcttt atcgatccgc caaccttctc taactcaaaa cgaatggaag atgcgtttga
    11281 tgttcagcgc gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg
    11341 gacgatcatg ttctcgaaca ataaacgtgg cttccgtatg gatctcgacg gactggcaaa
    11401 actgggactg aaagcacaag aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa
    11461 ccgccagatc cacaactgct ggctgattac cgcagcctga aaggaatagt aatgtcatta
    11521 atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa
    11581 ctgcatatcg aagataacga acgtgtttgt ctggtaggcc gtaacggcgc gggcaaatcg
    11641 acgttaatga agatcctcaa ccgtgaacag gggctggatg acgggcgcat tatttacgag
    11701 caagatttga tcgtggctcg tctgcaacag gatccgccgc gtaacgttga gggtagcgtt
    11761 tatgatttcg ttgccgaagg cattgaagaa caagcggaat atctgaaacg ctatcacgat
    11821 atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt
    11881 caggaacagc tggatcacca caacctgtgg cagctggaaa accgcatcaa cgaagtgctg
    11941 gcgcaactgg ggttagatcc taacgttgca ctgtcgtcgc tttccggcgg ctggttgcgt
    12001 aaagcggcat taggacgcgc gctggtgagt aatccgcgcg tgctgttgct tgatgaaccg
    12061 actaaccacc tggatatcga aaccatcgac tggctggaag ggttccttaa aaccttcaat
    12121 gggacgatta ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt
    12181 gtcgatctcg atcgcggcaa gctggtgacc tatccaggga attacgacca gtacctgctg
    12241 gaaaaagaag aagccctgcg cgtggaagaa cttcaaaatg ccgagttcga tcgcaaactg
    12301 gcgcaggaag aggtgtggat ccgccagggg atcaaagcac gccgtacccg taatgaaggc
    12361 cgcgtacgcg ccctgaaagc aatgcgtcgt gagcgtggcg aacgtcgcga agtgatgggt
    12421 accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt cgaaatggaa
    12481 gacgtttgct accaggttga cggtaagcaa ctggtgaaag atttttcggc ccaggttcta
    12541 cgtggcgaca aaattgccct gattggtccg aatgggtgcg gcaaaaccac gctgctgaaa
    12601 ctaatgctcg gtcagcttca agcggacagc gggcgtattc acgttggcac caaactggaa
    12661 gtggcttatt tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt gatggataac
    12721 cttgccgaag gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt attgggctat
    12781 ttgcaggact ttctgttcca tccgaaacgg gcgatgacgc cggtacgtgc gctttctggc
    12841 ggtgagcgga accgcttgct gctggcgcgt ttgttcctca aaccaagcaa cttattgatt
    12901 cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg aactgctgga agaactgatc
    12961 gacagctatc agggcacggt attgctggtt agccacgatc gtcagtttgt cgataacacc
    13021 gttacagaat gttggatctt cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat
    13081 catgatgccc gtggtcagca agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa
    13141 accgaagaag ccgccgcggc aaaagcagaa actgtaaaac gcagcagtag caaactaagc
    13201 tataaattgc agcgcgaact ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag
    13261 ctggaagccc tacagacgca ggtggcggat gcttccttct tcagtcagcc acatgagcag
    13321 acacaaaaag tgctcgccga tatggctgct gcagagcagg agctggagca agcctttgaa
    13381 cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa
    13441 cagagacagc tgacaacggt aaaattgcct gatgaactgg gtttatcagg catgaaaacc
    13501 aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat gtgcgaacat
    13561 catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc
    13621 cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg
    13681 gatgcccctc ggcagcgtcc caccgcctat gcgttggctg cactgttcat gctgttgctg
    13741 tccaacttgt ttccttttgt gaatatgaac gttgcaggag ttaccagtga aattacatta
    13801 ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac ctttttcctg
    13861 ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact ggtgaatcgc
    13921 gcggaattac cggtccgttt aaaagagcaa ctggcacggg tgctttttca actcaaaacc
    13981 tggggaatgg cggagatttt cctcgctggt gtgctggtca gtttcgttaa actgatggct
    14041 tacggcagca ttggggtagg cagcagcttt ctcccctggt gtttattttg tgtcctgcaa
    14101 ctgcgcgctt ttcagtgcgt tgatcgtcgc tggttatggg acgacatcgc cccgatgcca
    14161 gaactgcgcc agccgctaaa accaggcgtc acggggatac gtcaggggct gcgttcgtgc
    14221 tcctgttgta cggcaatcct tcctgctgat gaacccgtgt gcccgcgttg tagtaccaaa
    14281 gggtacgttc ggcgtagaaa cagcctgcag tggacactcg cgctgcttgt aacgtccatc
    14341 atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt aggctcgaag
    14401 atgccgtcga cgattctcgc tggggtcatt ctgttatgga gcgaaggctc ttatcccgtc
    14461 gctgcggtga tctttctggc cagtattatg gtgccaacgt taaagatgat cgccatcgcg
    14521 tggctgtgtt gggatgccaa agggcatggc aagcgcgaca gtgaaagaat gcatttgatt
    14581 tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt tatcgcggtg
    14641 ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat gggtgcatta
    14701 atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga cccgcgtttg
    14761 tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg aatctaataa
    14821 tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt
    14881 cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg gaccggaagt
    14941 gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg
    15001 tagcgttgac gtcggcgtgg ttgaaagcgc cacactggct gatgatttga cgcacgttga
    15061 aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagaca ccgtcttttg
    15121 ggtggtgaaa ccgcagattg gtcgcgaagg gattagcggc ctgggaacgc tgctgtctgg
    15181 agtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat acgatttgct
    15241 ggactcgcca ccgttggccc cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa
    15301 aaaagccggg cagctctcgc caggagatcc ggtgctgttc cgtggctatc gggtaggttc
    15361 ggttgaaacc agcaccttcg atacacaaaa acgcaatatc agctatcaac tgttcatcaa
    15421 tgcaccttat gaccgactgg tgaccaacaa tgttcgcttc tggaaagata gtggcattgc
    15481 ggttgatctg acgtcagcag ggatgcgtgt ggagatgggc tcattgacaa cgctgctgag
    15541 tggcggtgtc agctttgatg tgccggaagg tctggattta gggcagccag tggcaccgaa
    15601 aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt acaccgatca
    15661 cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt
    15721 agagttccgg ggtattcgcc tgggtaccgt aagcaaagtg ccattctttg cgccgaatat
    15781 gcgtcagaca tttaacgatg attaccgtat tccggtactg attcgtatcg agccagagcg
    15841 gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg aattgttgaa
    15901 acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggtgcac tgtatgttga
    15961 tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta atggttatca
    16021 gattatcccg accgttagcg gcggcctggc gcaaatccag caacgactga tggaagcgtt
    16081 ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca gtacgctttc
    16141 tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga acaagatcct
    16201 cgctagccag tcgatgcagc agttgccgac ggatatgcaa tcaacgttgc gtgaattgaa
    16261 tcgcagcatg cagggcttcc agcctggctc cgcagcctac aacaagatgg tggcggatat
    16321 gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa
    16381 gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga agagggcgaa
    16441 acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg cagctccggc
    16501 gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca aagtaccgcc
    16561 agccagggca atcgtctgtt atgggtagag caggtcactg ttcctgacta tctggcgggg
    16621 aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg
    16681 gccagcccgt tggatcaaca gttgcgcaac accctggttg ccaacctgag tacgcaactg
    16741 cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggacacgct caatgttacc
    16801 gtaacggagt ttaacggtcg ctatgatggc aaggtcattg tcagtggtga gtggctgttg
    16861 aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggagtgca aactcaggat
    16921 ggttacgatg agatggttaa agtactggcc agtgtctgga gtcaggaagc cgcttctatt
    16981 gcgcaagaga taaagcgtct accttaatta taaagatttg taaatataac cgtctccggt
    17041 atgttgcctg aggcggtttt tttgtttcta acgtgcggaa aaatttgttc ctcttcacat
    17101 tttttgtaca accgacatgc ccgtgtagct cacaaatatg acagtggcgt gaattttgcg
    17161 cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt tagtaatcac
    17221 tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag acaaaaacga
    17281 gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa
    17341 gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa
    17401 gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa acctccgcat
    17461 tgcggaggtt tcgccttttg atactctgtc tgattataat cagaaggcag acgtatcctg
    17521 gaacagaccg actttcaggt cgctggcggt atagataaga cgaccatcaa ccagcacttc
    17581 gccatccgcc aggcccataa tcagacgacg gttaacaatg cgtttaaagt gaatacggta
    17641 ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc caacgcccag
    17701 cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca gctgccacat
    17761 tgcgtccagg cccaggcatc ccggcataac cggatcgcca ataaagtggc atccgaagaa
    17821 ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgtcga agttaccacc
    17881 cgtttcggtc attttgacca cacggtccat catcagcatg ttcggtgctg gcaattgcgg
    17941 gcctttagcg ccaaacagtt caccgcgacc agaggcaaga aggtcttctt ttgtatagga
    18001 ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg caggatagct
    18061 aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag ccaacgtaat
    18121 ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg
    18181 gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa taacggcagt
    18241 gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac cgctttcacc
    18301 agttcactgt gaagacttaa atggcgaacg ttagctgtgg ggataatgac accttgtttc
    18361 ccggttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc atttaaacca
    18421 ccgaccggct gggcgcgacc gaactgatcg actgaacctg tgatagcgat actctgattc
    18481 accggcacat cggcgagggc gcttatcagg gcgcagagtt cagccatcga ggcactatct
    18541 ccatcaactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc
    18601 tcaagctgta gttccgacat caggaacgct tgcatgatca tcatcccttt cgcatggata
    18661 ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca
    18721 acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc
    18781 gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat ttgctcctga
    18841 aggatctcat cctgcatacg ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc
    18901 attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc gacctcttta
    18961 cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt
    19021 gctgcttcgc ggataagtac cggccaggca tccgctcccg gtgcaggtaa gtgattatgt
    19081 ctggcggtaa atgtcaccca gcgacaccac tgggttactg actctgcatc gacaatctgc
    19141 agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc catctcctgg
    19201 aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa
    19261 ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg
    19321 ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt acgcaaagag
    19381 atgatgagaa taccgccatt tgcctgatgc accagaccag gctgcagggt aatgtcgcca
    19441 ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc agcgacaact
    19501 tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg gatggagtgg
    19561 ccggaaacct catagtgacc gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg
    19621 gcattggcaa taagattcag atactcagac tcttccgggg ccttcgccag cataaaagag
    19681 gaggatgctc gcgtatgcag caactgctcc agtgcgaatt gcagtcgcgg ttgagtatca
    19741 ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc ctgatagcta
    19801 tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt tgatgttttt
    19861 tagtctgttg tcaaagccgc gattataccg taaccggcac tacagcacac gtagaaagca
    19921 ccgacaatac tcctggcatg ggcgttaaag ctcacaggat ggagattctt ttcttcactg
    19981 gcctaaaaag ctgatattct gtaaagagtt acacggtaac attgagatcg ctatgaaata
    20041 tcaacaactt gaaaatcttg aaagcggttg gaaatggaag tatctggtga agaagcatcg
    20101 cgaaggggag ttaatcaccc gttacataga agccagtgcc gcccaggaag ccgtcgatgt
    20161 gttgctctcg ctcgaaaatg aacccgtgct ggtaaatggc tggattgaca agcatatgaa
    20221 tccggaactg gtcaatcgca tgaagcaaac cattcgggca agacgaaagc gccattttaa
    20281 tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg gaatttattg tctggcaacg
    20341 tctggctggt cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg ttcagctgat
    20401 tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg tcttctctga agcaggatct
    20461 gcaggcattg ctgggtaagg aataactgac gaaagtcagt tcaatttact aaaggcaaaa
    20521 aaaaccccgc agcagcgggg tttttctacc agacgagaac ttaagcttgc ggctgagtta
    20581 caacgtcttt gataccttta acttcgatct ctacgcgacg atccggagcc aggcagtcga
    20641 tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc agtaaccggg ttggattcgc
    20701 ccataccacg tgcggagatc ttgtctgccg ggataccttt ggagatcagg taatcaacaa
    20761 cagactgagc acggcgctcg gacagaccct ggttgtaagc gtcagaaccg atgcggtcgg
    20821 tgtaacccag aacaactacg gaaccgtctt tcggatccag gttgctcagc tggctgtaca
    20881 gctgatccag agcagcctga ccttccggtt tcagggttgc tttgttgaag ttgaacagaa
    20941 cgtcagactt cagagtgaag tgcttggtct gtacttccgg tgccggagct ggagccggag
    21001 caactactgg agctgcttcg ccctgaccga aacggtagga aacacccagg ctcagcatgc
    21061 cgttgtccgg acgagtgccg atggtgtgtg cgtcaccgat gttgttggtc cactggtatt
    21121 ccagacgggt agcgatttca ggagtgatcg cgtactcaac accgccagcg aagaccggag
    21181 aaacgccggt gtcgtggttt ttaccataaa cgttggattt agtgtctgca cgccatacca
    21241 taccacccag acgagtgtag atgtccaggt cgtcagtgat tgggtaaccc agtttagcgg
    21301 tcagttgaac gccctgagct ttgtatgcac cgttttcaac gctgcctttg tacggcatac
    21361 gacctaacca gtcgtaaccc atttcaaagc caacatacgg gttaacctgg taaccaccaa
    21421 aagcaccagc gcccagttgg ttttcatggg tcgggccatt gttgttgatg aaaccagtgt
    21481 catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct ttcggagcgg
    21541 cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct gtctttttca
    21601 ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga gataacacgg
    21661 ttaaatcctt caccggggga tctgctcaat attaactcta ccgatatctt cggcttatgc
    21721 cgagcacccc tggcgatgta aagtctacaa cgtagttgaa aacttacaag tgtgaactcc
    21781 gtcaggcata tgaaa
//
LOCUS       DAZFVO020000084        21538 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFVO020000084 DAZFVO020000000
VERSION     DAZFVO020000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.084"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..136)
                     /locus_tag="ACHKNB_RS14445"
                     /old_locus_tag="pgaptmp_002889"
     CDS             complement(<1..136)
                     /locus_tag="ACHKNB_RS14445"
                     /old_locus_tag="pgaptmp_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4196366.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
     gene            complement(201..1212)
                     /locus_tag="ACHKNB_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /pseudo
     CDS             complement(201..1212)
                     /locus_tag="ACHKNB_RS14450"
                     /old_locus_tag="pgaptmp_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
     gene            complement(1243..1593)
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS14455"
                     /old_locus_tag="pgaptmp_002891"
     CDS             complement(1243..1593)
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS14455"
                     /old_locus_tag="pgaptmp_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4196367.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(1590..2015)
                     /locus_tag="ACHKNB_RS14460"
                     /old_locus_tag="pgaptmp_002892"
     CDS             complement(1590..2015)
                     /locus_tag="ACHKNB_RS14460"
                     /old_locus_tag="pgaptmp_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4196368.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            complement(2407..3450)
                     /locus_tag="ACHKNB_RS14465"
                     /old_locus_tag="pgaptmp_002893"
     CDS             complement(2407..3450)
                     /locus_tag="ACHKNB_RS14465"
                     /old_locus_tag="pgaptmp_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4196369.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLRRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            complement(3706..4971)
                     /locus_tag="ACHKNB_RS14470"
                     /old_locus_tag="pgaptmp_002894"
     CDS             complement(3706..4971)
                     /locus_tag="ACHKNB_RS14470"
                     /old_locus_tag="pgaptmp_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4196370.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSTLIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            complement(5169..5244)
                     /locus_tag="ACHKNB_RS14475"
                     /old_locus_tag="pgaptmp_002895"
     tRNA            complement(5169..5244)
                     /locus_tag="ACHKNB_RS14475"
                     /old_locus_tag="pgaptmp_002895"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5209..5211),aa:Phe,seq:gaa)
     gene            complement(5350..6057)
                     /gene="yqgA"
                     /locus_tag="ACHKNB_RS14480"
                     /old_locus_tag="pgaptmp_002896"
     CDS             complement(5350..6057)
                     /gene="yqgA"
                     /locus_tag="ACHKNB_RS14480"
                     /old_locus_tag="pgaptmp_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HGU4196371.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6210..6293
                     /gene="yqgH"
                     /locus_tag="ACHKNB_RS14485"
                     /old_locus_tag="pgaptmp_002897"
     CDS             6210..6293
                     /gene="yqgH"
                     /locus_tag="ACHKNB_RS14485"
                     /old_locus_tag="pgaptmp_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HGU4196372.1"
                     /translation="MHAISLHSLAYRHFARNVSLSMLLLMR"
     gene            6455..8590
                     /gene="speC"
                     /locus_tag="ACHKNB_RS14490"
                     /old_locus_tag="pgaptmp_002898"
     CDS             6455..8590
                     /gene="speC"
                     /locus_tag="ACHKNB_RS14490"
                     /old_locus_tag="pgaptmp_002898"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4196373.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(8640..9896)
                     /gene="nupG"
                     /locus_tag="ACHKNB_RS14495"
                     /old_locus_tag="pgaptmp_002899"
     CDS             complement(8640..9896)
                     /gene="nupG"
                     /locus_tag="ACHKNB_RS14495"
                     /old_locus_tag="pgaptmp_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HGU4196374.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10098..11177)
                     /gene="mltC"
                     /locus_tag="ACHKNB_RS14500"
                     /old_locus_tag="pgaptmp_002900"
     CDS             complement(10098..11177)
                     /gene="mltC"
                     /locus_tag="ACHKNB_RS14500"
                     /old_locus_tag="pgaptmp_002900"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HGU4196375.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11242..11517)
                     /gene="yggX"
                     /locus_tag="ACHKNB_RS14505"
                     /old_locus_tag="pgaptmp_002901"
     CDS             complement(11242..11517)
                     /gene="yggX"
                     /locus_tag="ACHKNB_RS14505"
                     /old_locus_tag="pgaptmp_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HGU4196376.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11545..12597)
                     /gene="mutY"
                     /locus_tag="ACHKNB_RS14510"
                     /old_locus_tag="pgaptmp_002902"
     CDS             complement(11545..12597)
                     /gene="mutY"
                     /locus_tag="ACHKNB_RS14510"
                     /old_locus_tag="pgaptmp_002902"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGU4196377.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            12758..13477
                     /gene="trmB"
                     /locus_tag="ACHKNB_RS14515"
                     /old_locus_tag="pgaptmp_002903"
     CDS             12758..13477
                     /gene="trmB"
                     /locus_tag="ACHKNB_RS14515"
                     /old_locus_tag="pgaptmp_002903"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGU4196378.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13477..13803
                     /gene="yggL"
                     /locus_tag="ACHKNB_RS14520"
                     /old_locus_tag="pgaptmp_002904"
     CDS             13477..13803
                     /gene="yggL"
                     /locus_tag="ACHKNB_RS14520"
                     /old_locus_tag="pgaptmp_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HGU4196379.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            13987..14706
                     /gene="yggN"
                     /locus_tag="ACHKNB_RS14525"
                     /old_locus_tag="pgaptmp_002905"
     CDS             13987..14706
                     /gene="yggN"
                     /locus_tag="ACHKNB_RS14525"
                     /old_locus_tag="pgaptmp_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HGU4196380.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            14882..15928
                     /gene="ansB"
                     /locus_tag="ACHKNB_RS14530"
                     /old_locus_tag="pgaptmp_002906"
     CDS             14882..15928
                     /gene="ansB"
                     /locus_tag="ACHKNB_RS14530"
                     /old_locus_tag="pgaptmp_002906"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HGU4196381.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16045..17052
                     /gene="yggM"
                     /locus_tag="ACHKNB_RS14535"
                     /old_locus_tag="pgaptmp_002907"
     CDS             16045..17052
                     /gene="yggM"
                     /locus_tag="ACHKNB_RS14535"
                     /old_locus_tag="pgaptmp_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HGU4196382.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(17207..18343)
                     /gene="hemW"
                     /locus_tag="ACHKNB_RS14540"
                     /old_locus_tag="pgaptmp_002908"
     CDS             complement(17207..18343)
                     /gene="hemW"
                     /locus_tag="ACHKNB_RS14540"
                     /old_locus_tag="pgaptmp_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGU4196383.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(18336..18929)
                     /gene="rdgB"
                     /locus_tag="ACHKNB_RS14545"
                     /old_locus_tag="pgaptmp_002909"
     CDS             complement(18336..18929)
                     /gene="rdgB"
                     /locus_tag="ACHKNB_RS14545"
                     /old_locus_tag="pgaptmp_002909"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HGU4196384.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(18937..19227)
                     /gene="yggU"
                     /locus_tag="ACHKNB_RS14550"
                     /old_locus_tag="pgaptmp_002910"
     CDS             complement(18937..19227)
                     /gene="yggU"
                     /locus_tag="ACHKNB_RS14550"
                     /old_locus_tag="pgaptmp_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HGU4196385.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(19224..19790)
                     /gene="yggT"
                     /locus_tag="ACHKNB_RS14555"
                     /old_locus_tag="pgaptmp_002911"
     CDS             complement(19224..19790)
                     /gene="yggT"
                     /locus_tag="ACHKNB_RS14555"
                     /old_locus_tag="pgaptmp_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HGU4196386.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(19808..20512)
                     /gene="yggS"
                     /locus_tag="ACHKNB_RS14560"
                     /old_locus_tag="pgaptmp_002912"
     CDS             complement(19808..20512)
                     /gene="yggS"
                     /locus_tag="ACHKNB_RS14560"
                     /old_locus_tag="pgaptmp_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HGU4196387.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            20530..21510
                     /gene="yggR"
                     /locus_tag="ACHKNB_RS14565"
                     /old_locus_tag="pgaptmp_002913"
     CDS             20530..21510
                     /gene="yggR"
                     /locus_tag="ACHKNB_RS14565"
                     /old_locus_tag="pgaptmp_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HGU4196388.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
BASE COUNT         5349 a         5307 c         5591 g         5291 t
ORIGIN      
        1 gttgtgattc atacaggctg agttcacggg ctgcggcagt aacaccgatg cgttcagcaa
       61 gcttcagggc ttcactgcga aattcaggcg aatgctgttt acggggtttt ttactggttg
      121 atactgtttt tgtcatgtga gtcacctctg actgagagtt tactcactta gccgcgtgtc
      181 cactattgct gggtaagatc actgaacaac ctgtcgtaac ctgtgaacgc atccgcctgc
      241 aggatacccc ggaaggggcg gaggtgttgc tccgggtgtt tcccctgccg gttcggcgag
      301 tacgcgaacc agaccgctgg aggagatgac gaacccacat tgcgatcatc ccggacatac
      361 gtccagatac gccctgtttt cgcctttttc tgacccggtg ccagtacctt taccggtgtg
      421 tcatcagtgt gaaccttgcg ggtgttcatt acataacggt acagggcatc attcaccggt
      481 gtcattaact ggcagcacgc gtcaacccag ttggagagta aggcccggct cagttcgaca
      541 ccctggcggg caaagatttc actctgacga tacagtggca gatgttcgca gtattttccc
      601 gttaacacgc gggcaagtaa tccggggccc gcgataccac gctctatcgg gcgggacggc
      661 gccggtgctt caacaataca gtcacatttt gtacaggctt tttttacccg ttctgtgcgg
      721 atcactttca gggcactgct caccagttcc agctgttcag cgctgacttc ccccagataa
      781 tccagctcac cgccacactc cgggcaacag ctttcttctg gctccaggcg gtgtatttca
      841 cggggaaggt gtgccggtaa cggacgacga tggcgtgact gtcgcaactg gcggggaacc
      901 tgaggatcgt cttcccgccc actgtaacga tcgctgtccc agcagagcct cagccagttc
      961 aacttcacga cgcagttttt cagaacgggt accgaacagc atccggcgca gtttttctat
     1021 ctgagcccgc agatgttcta tttcccgttc atcttcttcg atcttttctt cggcacgtgt
     1081 cagtgcagag cgcaggaagg cttccgtctc ttcaaccaga ctcagttgct ggtctttctg
     1141 acggagggct tcagcctgct cagagagcaa cctttccagc tctgcgatgc gaatgaggta
     1201 tttctgactc atgaccgttt ttataatgcg gtcaggagtt ttttacaaca ttgtcagtga
     1261 gttacggctg gatgtttttg gctgacgcca gtccagctta tcgaggagca ttgccagttg
     1321 cgagcgggta atggatacct tgccgtcacg taccgcaggc cagataaact ggccttcctc
     1381 caggcgtttg gtgaacaggc acagaccatc agcatcagcc caaagaattt tgacggtgtc
     1441 accccgtcgg ccacggaaga taaacaggtg accggagaag ggattatcat tcagcacatg
     1501 ttgtacctgt tctcccagtc cgttgaagga tttacgcata tcggtaacgc cggcaacgag
     1561 ccagatacgg gtacctgatg ggagtgagat catcttcccc tcccggtcag ttcacggatc
     1621 aacactgtga gcagctctgg cgatggattt tccagcgtca tgttaccgtg acggaattcc
     1681 accttgcagg aactggcact gactctggtc tgagtggaag tggataaaga cggcgcaatg
     1741 gccgccacag gttctttctg ctcatccggc gttatttcta caggtaataa ttcaacgcca
     1801 gtgtcagaag aggtcgttac cggaagacgc cgcgaaacac gcccttcgtt ctgccagagc
     1861 ctgagccatt tgaaaataac attatcattg acgccatttt cacgtgcaat ctgtgcaaca
     1921 caagctccag gttgtgatgc cagttccacc atacgaagtt tgaattcatt cgaatagttt
     1981 ttacgaggtt cttttcgcca gtcctgtaat tccatactta gatgtccgtc tatatcagat
     2041 gggcgtctaa gttaccaatt ctcgtctgat ggctacatac ggcggtcagt ttacgcttac
     2101 gattatcttg ctaattacca gactggagta accagaccaa ccgaaaacac cggggaggtg
     2161 attacccgct ggcagaggaa atccggtttt tttgatgtca gccataacgt gtttgacaat
     2221 aaagcattat gaaagggtgt taatgagtcc atgggataat tcataaaaat aatttactgt
     2281 gttatccttt tcaaaagaaa tattcagaag ataacccctg gctgacaacc aggggaaggg
     2341 gacattaaca gtgttattat tggtggataa attattactt gtgtgcagga tacactaatg
     2401 caatatctac tgatactgta tgccagtatg tttcatgcct gccgctgaaa acggcaatat
     2461 ccagggccag acatattcac cattcatatt tctgcatttc tgtacaattt tctcagaaag
     2521 cgtctcatca attctttcac gcaacttctc ctgctgtctt aaaatacttt cgcatatttc
     2581 gaaatcatct tcctgcatca gcatttcggc gcatttaatg atttctgccc gttcatgcag
     2641 cacagaggaa tgattataga gccagtttac aaacagcaat gcttccggat attgccccca
     2701 taacgggtgg tatgtggcat aataatcttc ggcagtatcc ggaccgcctg aaaagttatg
     2761 ccatacagca agtggaaatg gatgcagatt tccgcacagt atttctgcga tgtaagtatt
     2821 tgccgcaaag ccgcgacgaa atgcagttgc agcttttacc agttccccat taagaaaatg
     2881 cagaaggccg agttcatacc agtaaggtga aaattcatct gcatgttcct gcagtatatg
     2941 gcgtgcctgt tcatgagcgc cagtgcgtaa cagttccggt cccagtagcc agcgtgcacc
     3001 atgattatct tcgggattgt aatccagtat tttttcaatg agcatgatgg catcctgatg
     3061 acgccgtaaa tgtgcatttg ccaggattgc cgcatagagg gcacgaagaa aaggtcggtt
     3121 gtcaggatgt atccagatga ttctgccact gaaaccttca gggataagac gattgccaac
     3181 tgccagccct ttaagggcag cattcagcgc cttacgcggg gcattctgct ccagaaatac
     3241 gtatgccaag tgagcgtgta catcaataaa gtcattctcc tgtcgggcaa ggcgcctgag
     3301 tttgttgatg taacttgttt cgctgatttc atccgcatcg tatgcatcaa tcagttcttc
     3361 aaactcatcc agcaacgagc caaaccaggt ttccggaaat atgaaacagc cctggttatc
     3421 gttcacttca aagcgtaatt tgccagtcat attctgaacc tgtaaaaaag gatggaccat
     3481 aatctgcagg ctataaaaat tgtgatgcct ggcatcggtg tccttttatt gtccgggatt
     3541 aacgttgccc atgataatac agtgaatcct gttctgtggt aagacgttga gttattgaat
     3601 gagaacctca gcctggctga tggttccgcc ggcataaaaa agaaattgat tgaggcgggt
     3661 gctcttcccc ttaagaggaa gagcatgtgt gtaaatggtg ttcagtcacc ttttcagtgg
     3721 attgttaact tttgcatatt caaacggact gatacactcc tccctgtttg catccagaaa
     3781 atctgcccac cattgcagca taagccgacg ttcttccaga tgttctgctt tatgaatata
     3841 cgcggcacgt actccgttgc gctcctgatg actcatctga cgttcaaccg catctcttga
     3901 ccataagccg gattcaatca atgtactgca cgccatcgcc cggaatccat ggccacagac
     3961 ttccttggtt gtgtcatacc ccatcactct gagggcctta tttacggtat tttcactgat
     4021 tggtttacgc gggtcatgtg ctcccgtaaa gatcagacca ttttcaccag cccatgtctg
     4081 aagttctgtc agtatggcta cagcctgttt gctgagtggc acaagatgtg gtgttcgcat
     4141 cttggagccc cggtgtgaga attttacccc tggcagaggt ttgcgttcgg ctggaatagt
     4201 ccacatggca ttgtcaatat caatttcaga ccagcgggcg tatctgagtt cactggaacg
     4261 aataaaaacc agtaaattca gcataacggc aagccgggtg acaggctgac ctttataact
     4321 gttaatacgc gacagaagtt caggcagacg tgaaagatca agagcggggc gatgctggcg
     4381 ttttaccgta gtcaatgcgc cagccatatc cagagcagga ttatatctga tgatgccaga
     4441 ctgtacggca taacgcatta cggcagatat tctttgctgc agacgactgg cgatttcata
     4501 aatttgttta gcttctgcag cacgaacagg gataagcaga tccggggtat tgagtgttgt
     4561 gatatcccgg ttgccgataa aggggaaaac gtgtgtttcg aggcttttga gtactttatc
     4621 gctgtgtgat tgtgcccatt gtttattact ggcatgccag cgtcgtgcaa tatgttcaaa
     4681 agagagctgt aaatcaggga ctactttttt agctcttttc tgttcgcaag ggtcaatccc
     4741 ctccgaaatc agttttcggg cctcatcccg ttttgtacga gcttcggaga gagaaacctc
     4801 cgggtataca ccaagtgcca gagtcttctc cttacccaga atacgataac ggagacgcca
     4861 gtaacgggaa ccattaggat gtaccagcag gaacatgcca gccccgtcag tgagtttata
     4921 agccttgtca gtaggctttg ctgcccggat ttttgcgtca gtcagtgcca tagttcagcc
     4981 ttctgaatgc gttaaagagg gttcaaattc tgtcgaactg gggtatgccc acaattatac
     5041 ccacacaatg aggttgatgt ggggggaatc tggttgagtt cggtagaatt gatttggagg
     5101 caggacgctt aaatcgtggc gtcctggaat gaaaaacgga cctccgtgga ggtccgttga
     5161 tatgaatttg gtgcccggac tcggaatcga accaaggaca cggggatttt caatcccctg
     5221 ctctaccgac tgagctatcc gggcaacggg gcgcattaaa cctgattcgc ctcgtctcgt
     5281 caatcaaatt tcttcaattt actgcagact gcacaatcta tcatcacttt gccgctattg
     5341 cacgcattgt caggcaaacc aggcggtcca ggcggcggaa agcggcattg ccagaaggag
     5401 tgctggaagc atgttgacca ccgggaacat tttaatgcca cagatgcgta atccggttgc
     5461 cagcagcaat aaaccgccta cggcgctgaa gtctgccatc atcgacggtg tggtcagcgg
     5521 taatatcagc gcggcagccc acgccagcgt taactggatg atcagtaatg ggatactaat
     5581 taccgacacc gcaataccca gcgagcaggc gaagatcatc gccgtaaaga aatcaagaaa
     5641 tgacttggcg attaaaatac tcggatcgcc ggtcatccct tcgttcatcg ccccgaagat
     5701 cccggtgccg ctggcgcaaa acaggacaat aatcgcgaca taattctgaa taaaagattc
     5761 atgcgctggc ttcttacgtg agtgacgaaa cagattttgt gctttggcga ctgctgtatt
     5821 gacacctttc tccagcaaac aaatttcgcc aattagagcg ccaagtagcg tagctaaaac
     5881 catcgctgga aggttggcgc atttcacaac cagtaaaata ccaatcccca gcgatgccag
     5941 accaaaaata gatgtcatgg agacgcggat acgttccggt aagcgttggc tgagcagtgc
     6001 gccgagaacg ccacccagta agactgcact agcgttgata aaagggccga tgaccacaag
     6061 agttcctgtt agctgtattg tttgattttc ttattatgaa ctttttgcct tgttgatggc
     6121 ttgtctggtc gcaaagtcct ggcttgcacg ctttagcgaa aggtgccatg attgcgcgaa
     6181 ttttctcctc tctgtacgga gtttgcccga tgcacgccat ctccttacat tctctcgctt
     6241 atcgccattt cgcgcgaaac gtttcccttt ctatgttact gctcatgcgg tgaagttaac
     6301 gcacgctcac tgcaggacaa cagtaaaatc agagcgtttc tgcttttact gatgtctggc
     6361 ggtcggagct ggtgaccagt ttgacccata tctcatgggg cagggttttc caccttgtcc
     6421 ggtattctta cttccccgaa acgggtttgc gcttatgaaa tcaatgaata ttgccgccag
     6481 tagtgaactg gtatcccgac tttcttctca tcgtcgcgtg gtggcgttgg gagatactga
     6541 ttttacggac gtcgcggcag tcgtcattac cgctgcggat agtcgcagtg gcattcttgc
     6601 gttgcttaag cgcaccggtt ttcatctacc ggtgtttttg tattccgaac atgctgttga
     6661 attacctgcg ggcgttacgg cggtaatcaa cggcaacgag cagcagtggc tggagctgga
     6721 atccgcagcc tgtcagtatg aagagaattt gctgccaccg ttttatgaca cgctgacgca
     6781 gtacgttgag atgggcaaca gcacctttgc ttgccctgga catcaacatg gtgcgttttt
     6841 taaaaagcat cctgccggac gccattttta cgatttcttt ggtgagaacg tctttcgcgc
     6901 cgatatgtgt aacgctgacg taaaattggg cgatctgctt attcatgaag gatcggcgaa
     6961 agatgcgcag aaattcgcag ccaaagtctt tcatgccgat aaaacctatt ttgtgctgaa
     7021 cggcacatcg gcagcgaata aagtggtgac gaatgcgctg ttaacgcgtg gcgatctggt
     7081 gctcttcgac cgtaacaacc ataagtcgaa tcatcacggc gcgctgattc aggcgggggc
     7141 gacgccggtc tatctggaag cttcacgcaa cccgtttggt ttcattggcg gtattgatgc
     7201 gcactgtttt aatgaagagt atctgcgcca gcaaattcgc gacgttgcgc cagaaaaagc
     7261 cgagctgccg cgcccgtttc gcctggcgat tattcagctg ggaacctatg acggaactgt
     7321 ctataacgcc cgtcaggtga tcgataccgt tgggcatctg tgtgattaca ttctgtttga
     7381 ttccgcgtgg gtcggttacg aacaatttat cccgatgatg gcggatagct cgccgctgct
     7441 gttagaactt aacgaaaacg atccggggat ttttgtgacc cagtcggtgc acaaacagca
     7501 ggctggattc tcacagacgt cgcagatcca taaaaaagat aaccatatcc gcggacaggc
     7561 gcgtttttgc ccgcataagc ggttgaataa cgcctttatg ctccatgctt ctaccagccc
     7621 tttctatccg ctgtttgctg cactggatgt taacgccaaa attcatgaag gggagagtgg
     7681 gcgtcggctg tgggctgagt gcgttgagtt gggtattgag tcgcgcaagg ctattcttgc
     7741 gcgctgtaag ctgttccgcc cgtttatccc gcccgttgtt gatggcaaat tgtggcagga
     7801 ttatccgaca tctgtgttag ccagcgaccg ccgttttttc agttttgagc cgggggcgaa
     7861 gtggcacggc tttgaaggat atgccgcgga tcagtatttt gttgatccgt gcaagctgtt
     7921 actcactaca ccaggtatca atgccgaaac tggcgaatat agcgactttg gcgttccggc
     7981 gacgattctg gcgcactatc tgcgtgagaa cggcattgtg ccggagaagt gcgatctcaa
     8041 ctccattctg ttcttattaa ctccggcgga aagccacgag aagctggcac aactggtggc
     8101 gatgctggcg caatttgaac agcatattga ggatgactcg ccgctggctg aggtgttgcc
     8161 gagcgtttat aacaaatatc cggtgcgcta tcgcgactac accctgcgcc agttgtgtca
     8221 ggagatgcac gatctgtatg tcagtttcga cgtcaaagac ctacaaaaag cgatgttccg
     8281 ccagcagagt ttcccgtcag tggtgatgaa cccccaggat gcgcatagcg cttatattcg
     8341 cggtgaagtg gagttggtgc ggattcgtga tgccgaaggg cgaattgcgg cagaaggggc
     8401 gttgccttat ccacctggcg tgctttgcgt ggtacccggg gaagtctggg gtggggcggt
     8461 tcaacgttat ttccttgcac tggaagaagg ggtgaatttg ttgccgggat tttcgccgga
     8521 gctgcaaggt gtttatagcg aaaccgatgc ggatggcatg aaacggttgt acggttatgt
     8581 gttgaagtaa gaataaaaaa aacgggtcac cttctggcga cccgtttttc tttgcgtaat
     8641 tagtggctaa ccgtctgtgt gcctgtcggg acacgaacgt gtttatattt gaacatcgcc
     8701 atgaacgcga aggccagaac cacggagtaa ccagcgaaaa tcaaccatac ggtctgccag
     8761 tcggtaatgc cgttttgggt gtacatctca acaactttac cgctcacgat gccgccgagg
     8821 atacagccga agccgttagt catcatcagg aacatccctt gtgcactggc gcgaattgcc
     8881 gggctaactt ctttttcgac aaacaccgaa ccagagatgt tgaagaagtc gaatgcgcaa
     8941 ccgtaaacga tcatcgacag taccagcagt acagtaccga acggagtcgg gtcgccgtaa
     9001 gcaaacagcg caaaacgcag gatccacgcc acaatactga tcatcattac gttcttaata
     9061 ccgtagcggc ttaagaagaa cgggatggtc agaatgaaca gggtttcaga gatctgcgaa
     9121 atcgacatga tgattgacgc atgctgcaca ataaagctgc tggcaaacat cggatctttg
     9181 tcgaagctgt gcaggaaggt attaccgaac atgttggtaa tctgcagttc cgcgcccagc
     9241 agcattgaga agataaagaa gattgccata cgcttgtttt taaacagcgc gaatgcatcg
     9301 aggcccagca gggttgtcca gctctgattc gcttgctgtt tagcaaccgg aatatgcggc
     9361 agagtcaggg taaacagaac cagaatggcg gaaagtgctg cgccaatata cagctgcatg
     9421 tggcttaatt cgaagccaga caggctcacc acccacattg ccatgataaa gccgatggtg
     9481 ccccagatac ggattggcgg gaagtcagta acgatatcca tcccggcatt ttgcaggcga
     9541 tagtaagaga tggtgttgat taacccaagc gttggcatat aagcaaacga gttaatcaat
     9601 atcacaagga acatcgcctc cggtgtcgtg acctgtgccg ccatgaacag cgtgatagcg
     9661 ccaatggtgt ggcaaatggc atatacccat ttcgcactta accatttgtc ggccacaatc
     9721 cccagcagcg caggcataaa gaccgcggcg atacccagtg agctataaac tgcgccaata
     9781 gaagcaccgt caaacttcag ggtaacaaac atataggagc cgagggtcgt cagccaactt
     9841 ccccacagac agaactgcag aaaagagagg attttcagct gcagcttaag attcatgtta
     9901 atttcctcac atcgtgatgc ggatgagtgt ttgaaaaagc acatttgacc gtgcgagatg
     9961 atgggattct atcaacttcg cggactggtt ttttgttacc tgtggcaaat aattgcaaac
    10021 attcccaatt tgcaaaacga aattgtgatg tggataacat ttttgcccct gagcatcgtc
    10081 aggggcggtt aatggaatta tcggcggcgg taggattttt gcgcggtatt cactttataa
    10141 agataacggc gagattccgc agagggatgg cgggtcgtca gcgtctgata aacatcgccc
    10201 ggcgtcatgg tgttaataat attggcagcc tgaatcttat cattcgaaaa gactcgcagc
    10261 acgctgcctg cgccgccgtt ataggcggtg atgacggcat aacgccgcga tgttgggtta
    10321 tcaattccgc cgagataaac attgttcagc atcgccagat acgcggtgcc ggtatcaata
    10381 ttgctggcag gatcaaacaa gaaactgcgg ctcggcgtgc cggatttccc ctgcgagcgg
    10441 aacacatctt tcccggcagt atgttgtacc acctgcatta atcccagcgc atcggaacgg
    10501 ctgaccgcat acgggttaaa ggaagattcg gtctgcataa ttgccagaat cagcgactca
    10561 tcaacgccat atttccgtga cgcctggcgg accatgccga gatatttgtg cgcacgttta
    10621 tcaaggtggt tcggtaccat gttaatggtg acgctgtaga tgatacgcag cccgttattg
    10681 cggctttgca gacggttttt cagcagataa tccgcgaagt tgcttgcgcg accttcccag
    10741 cgaatcggct gcccggtgtt gtccaccacc tgaccgtaaa ggaaaggttc tttcgaaatc
    10801 gtaatatcat caacgtcgga atagagatcg accgaactcg gatcgtcacc catcagcaac
    10861 gttttgataa ttgcccggcg caaatgcgcg gcaggttctg tcccggcgat agtttcgata
    10921 gtaatcgtac cgtcatcgaa gttgatgtgg ctgcgggtct gatattgatc ggtgtatttc
    10981 acgtagtcct taggaccggc gatcaccacc tctttgaatc cccagatgtt ctcaatattg
    11041 tgggcaaatt gccccatcag aatatcaaaa ccgttggtat ctttgaccca ggcttcgtta
    11101 taggtatcgc cttttttggt cgtcgaacag gagatgagca acggcgcaat caaagccagc
    11161 gcgagatatt ttttcatcat tccgggtgcg tgttgtgttt gtttttatgc aagttgccgg
    11221 aggcgtgctc cggcactgtt tttatttttt atcttccggc gtatagccct cgatatgcac
    11281 ctctttaccc tcgaacagga agttgaccat ctcctgctca agcagcttgc ggtgctcggc
    11341 attcatcatg ttgagtttct tttcattaat cagcatggtt tgcttgtgct gccactgcgc
    11401 ccaggcttct ttggagatct cgttatagat gcgttttccc agctcgccgg ggtacagctg
    11461 aaaatcctga ccttctgctt cacgttgcag gaaagtacaa aaaatcgttc tgctcataaa
    11521 tcatcctctt tatcgactca cgcgctaaac cggcgcgcca gtgcgtaact gctgtaacaa
    11581 acgctccacg ggagccgcca ggccaactga cggcggttgc gctaagttat accagagcgc
    11641 attgccttca tccatgcagc cggtgaatga cgacacggga agccacatag gcacaatatc
    11701 taagtggaaa tggctgaagg tatggcgaaa cgcggtcagc tgcgtcaggt tatcggcagc
    11761 aatctgccgt tgcgccagcc aatgccgcaa actttcttcg tcggcaaact gcgggaaaca
    11821 gtataaaccg ccccacaatc cgctcggcgg acgctgcgcc agcaatactt catcttcgtg
    11881 ctgtaatagc aaaaagtagc cggtgcgctc cggcagcgtc tgtttcggtt ttttgcccgg
    11941 ataaagcgac cagctattgt tggcggcggc aatacatccg ttttgtagcg gacagagcga
    12001 acatttcggt ttcgagcgcg tacaaatcat cgcgcccaaa tccatcatcg cctgattaaa
    12061 ccgttccacg ccgaccgcgg gcgtcacctg ctcgcttaaa ctccataatt tattctcgac
    12121 ctcttttttc ccaggccagc cgcttacagc atagcagcgc gccagcacgc gtttgacgtt
    12181 accgtcgaga atcggaaagt gcttacccag agaaagcgag agaatcgcgc ctgcggtgga
    12241 acgcccgacg cccggcagtg ctgcaacttc ctcaaaggtt tccgggaatt taccgccgtg
    12301 taaggtcgcc acttgttgtg ccgctttatg cagattgcgc gcgcgggcgt aatagccaag
    12361 cccggtccac aagtggagaa cttcgtcgag cggcgcattg gcgagatcgg tcaccgtcgg
    12421 gaagcgcgcc ataaagcgtt caaaataggg gataacggtc gcaacctgag tttgttgcaa
    12481 catcacttct gagagccata ctttgtaggg cgtcttgtca atttgccagg gcagagtttt
    12541 tcgcccgtat ttatcgtacc agtccagaac ctgggctgaa aattgcgacg cttgcatggt
    12601 caccgaattc actgttgttg ggggcaagat tgcagcacag cgacggcagg gtgtaaaccg
    12661 gaactttccg cagccacggc cttatcatgc ttgcatccgg caactaactt tggataatgc
    12721 ccgttttcag aacactttca caagcgacta aatctttatg aaaaacgacg tcatttcacc
    12781 ggaatttgat gaaaacggcc gcccactgcg ccgtatccgt agttttgtgc gccgccaggg
    12841 gcgactgacc aaaggccagg aacatgcgct ggaaaactac tggccggtga tgggcgttga
    12901 gttcagcgaa gatatgctgg atttccccgc gctttttggc cgtgaagcgc cggtgacgct
    12961 tgagattggt tttggcatgg gggcgtcgct ggtggcaatg gctaaagatc gccccgagca
    13021 ggacttcctc ggcattgaag tgcattcacc gggcgttggt gcgtgcctgg cttctgcgca
    13081 tgaagagggc ttaagcaacc tgcgcgtgat gtgtcacgat gcggttgaag tgctgcataa
    13141 aatgattcct gacaattcat tgcgcatggt gcagctcttt ttccctgacc cgtggcacaa
    13201 agcgcgccat aataaacgcc gtatcgttca ggtgccgttt gccgaactgg taaaaagcaa
    13261 actgcagctg gggggcgtat tccatatggc gaccgactgg gaaccttatg cggaacatat
    13321 gcttgaagtg atgtcttcta ttgacggtta taaaaacctg tcagagagca atgattacgt
    13381 accgcgtccg gcatcacgtc cagtgacgaa atttgaacaa cgtggtcatc gtcttggtca
    13441 cggagtatgg gacttaatgt tcgagagggt gaaataatgg caaagaaccg tagccgtcgt
    13501 ctgcgtaaaa aaatgcacat cgacgaattc caggaattag gattttcggt ggcatggcga
    13561 ttcccggaag gtacatcgga agaacagatt gataaaaccg ttgatgattt tattaacgag
    13621 gttatcgaac cgaacaaact ggcctttgac ggcagcggtt atctggcctg ggaaggtctg
    13681 atctgcatgc aggaaatcgg caaatgcacc gaagaacatc aggcaattgt gcgtaagtgg
    13741 ctggaagagc gcaaactgga tgaggtacgc accagcgaac ttttcgacgt ttggtgggac
    13801 taagaaagca tacgggcgat gacaaatgca aaactgcctg atgcgctacg cttatcatgc
    13861 ctggaaagat gcacgatcga gtaggccgga taaggcgttt acgccgcatc cggcatggaa
    13921 aacgcgtact ttgttatcaa tctggggcca gcaaatgctg gcctgatttg ttcttgaggg
    13981 aagactatga tgcgcaaaat gctgctggcg gcagcacttt cagtgacggc aatgaccgct
    14041 cacgccgact accagtgcag cgtcacgccg cgtgacgatg tgattgtcag cccgcaaacc
    14101 gtgcaggtga agggcgaaaa cggcaatctg gtgatcacgc cagacggcaa cgtgatgtat
    14161 aacggtaagc aatattccct gaatgccgcc cagcgcgagc aggcgaagga ttatcaggct
    14221 gaactacgta gcacgctgcc gtggattgat gaaggcgcga aaagccgcgt cgagaaagcc
    14281 cgtattgctc tggataaaat tatcgttcag gagatgggcg aaagcagcaa aatgcgcagc
    14341 cgtctgacca aacttgatgc gcagctgaaa gagcagatga accgcattat cgaaacgcgc
    14401 agcgatggcc tgacgtttca ctataaagcc attgatcagg ttcgcgccga aggccagcaa
    14461 ttagtgaatc aggcaatggg cggaatttta caggacagca ttaatgaaat gggcgcgaaa
    14521 gcggtgctga aaagcggcgg taacccatta cagaatgtgc tgggaagcct gggggggctg
    14581 caatcctcaa tccaaaccga gtggaaaaag caggaaaaag atttccagca gtttggcaaa
    14641 gatgtttgta gccgtgttgt gactctggaa gatagccgca aagccctggt cgggaattta
    14701 aaataattct ctattttaag acggcataat acttttttat gccgtttaat tcttcgtttt
    14761 gttacctgcc tctaactttg tagatctcca aaatatattc acgttgtaaa ttgtttaacg
    14821 tcaaatttcc catacaaagc taagggataa tgcgtagcgt tcacgtaact ggaggaatga
    14881 aatggagttt ttcaaaaaga cggcacttgc cgcactggtt atgggtttta gtggtgcagc
    14941 attggcatta cccaatatca ccattttagc aaccggcggg accattgccg gtggtggtga
    15001 ctccgcaacc aaatctaact acacagcggg taaagttggc gtagaaaatc tggttaatgc
    15061 ggtgccgcaa ctgaaggaca ttgcgaacgt taaaggcgag caggtagtga atatcggctc
    15121 ccaggacatg aacgataatg tctggctgac actggcgaaa aaaattaaca ccgactgcga
    15181 taagaccgac ggcttcgtca ttacccacgg taccgacacg atggaagaaa ctgcttactt
    15241 cctcgacctg acggtgaaat gcgacaaacc ggtggtgatg gtcggcgcaa tgcgtccgtc
    15301 cacgtctatg agcgcagacg gtccattcaa cctgtataac gcggtagtga ccgcagctga
    15361 taaagcctcc gccaaccgtg gcgtgctggt agtgatgaat gacaccgtgc ttgatggccg
    15421 tgacgtcacc aaaaccaaca ccaccgacgt agcgaccttc aagtctgtta actacggtcc
    15481 tctgggttac attcacaacg gtaagattga ctaccagcgt accccggcac gtaagcatac
    15541 cagcgacacg ccattcgatg tctctaagct gaatgaactg ccgaaagtcg gcattgttta
    15601 taactacgct aacgcatccg atcttccggc taaagcactg gtagatgcgg gctatgatgg
    15661 catcgttagc gctggtgtgg gtaacggcaa cctgtataaa tctgtgttcg acacgctggc
    15721 gaccgccgcg aaaaacggta ctgcagtcgt gcgttcttcc cgcgtaccga cgggcgctac
    15781 cactcaggat gccgaagtgg atgatgcgaa atacggcttc gtcgcctctg gcacgctgaa
    15841 cccgcaaaaa gcgcgcgttc tgctgcaact ggctctgacg caaaccaaag atccgcagca
    15901 gatccagcag atcttcaatc agtactaatc acctcgcccc ggtatcgtgc cggggctttt
    15961 tcacttcaga cttacgtcca ttgccaattt tcattaccct aatgataatc accggaataa
    16021 attattccgc gtgaggtttt tcgggtgaaa aagcaatgga ttgttggcac ggcactgctt
    16081 atgttgatga ctggtaatgc ctgggcagat ggcgaaccgc caactgaaaa tatcttaaaa
    16141 gatcaattca aaaagcagta tcacggcatt ctcaagcttg atgctatcac cttaaaaaat
    16201 cttgatgcta agggtaatca ggccacctgg tcagcggaag gcgatgtctc ttccagtgac
    16261 gatctctata cctgggtcgg tcagttggca gattacgaac tgctcgaaca gacctggacg
    16321 aaagataaac cggtaaaatt ctcggcgatg ttaaccagta aaggaacgcc cgcgtctggc
    16381 tggtcggtga acttttactc ttttcaggcg gcagccagcg atcgtgggcg ggtggtcgac
    16441 gatatcaaaa cgaataataa atatctgatc gtgaatagcg aagatttcaa ttatcgcttt
    16501 agtcagcttg aatctgcgtt gaatacccag aagaactcga ttcctgcgct ggaaaaggaa
    16561 gtgaaagcgc ttgataagca aatggtggcg gcgcagaaag cagcggatgc atattgggga
    16621 aaagatgcaa acggtaagca aatgacccgc gaagaagcct ttaaaaaaat tcaccaacag
    16681 cgtgatgagt ttaataaaca gaacgatagc gaggcgttcg cggttaaata tgacaaagag
    16741 gtttatcaac cggcgattgc ggcatgtcat aaacagagtg aagagtgtta tgaagtgccg
    16801 attcagcaga aacgagattt cgatatcaac gaacaacggc gacagacttt tctgcaatca
    16861 caaaaactca gccgtaaatt gcaggatgac tgggtaacgc tcgaaaaagg gcaatatccg
    16921 ttaacgatga aagtttcaga aataaacagt aaaaaagtcg cgattttgat gaaaattgac
    16981 gatatcaacc aagctaatga acgctggaaa aaagataccg aacaactgcg gcgcaatggg
    17041 gtgattaagt aagtttgata tagtgcacga tttgtagcag gatgcggcgt tgaccccgca
    17101 tccggcaatc aacgtctgat gcgacgcttg cgcgtcttat cagacctaca accgttgcca
    17161 catgtaggct ggataaggcg ttcacgccgc atccggcaat acaagtttac tcagccagaa
    17221 aaagctccag cagcgaattt aaaaacagct tcccatgttc cgttatctgc cagtaatccg
    17281 cacattctgt gagataaccc tgggcaatcg cctcgtctaa ctgtgggcga atcacatctt
    17341 cgcaaagccc ggtatacgca ataaactcca cgcgcggcgc ggcctccagc agacggaagc
    17401 gattcataaa gaactcaaac ggcttatctg cggcttcgac atcacgctgg ctttccagat
    17461 accttccttg cataaaacca cgcggatgac gcgttttggt ggtacgcaga atgcgcccat
    17521 ccgggaaggt cactttcccg tgcgcgccgc agccaatacc gatgtagtcg ccaaagcgcc
    17581 agtagttgag attgtgctgg cactgataac cgggtttggc gtaagcggaa gtttcatatt
    17641 gctgataacc cgctgcggtt aataactgat gcccctgttc gaaaatatcc cacaacgcat
    17701 cgtcgtccgg cagcaccggt ggtcgcgaac caaacagcgt attgggttcg atggtcagtt
    17761 gataccagga aagatgcggc ggattcagtt caatggcctg gcgtagatcg ccaagcgcct
    17821 cttccagtga ttgatccggt agtccatgca tcaaatcaag gttaaagcta cgtaacccta
    17881 aaccgctcgc cagctttgcc gctcgtttcg cttcttgcgg gccatgaata cgcccaagtc
    17941 gtttcagctt ttcttcgcta aaactctgca caccaataga gatgcggttc acaccagcac
    18001 gctgataatc gacaaagcga tccgcttcta ccgtgcctgg gttcgcttcc atagtaattt
    18061 ctgcatccgc tgccagcggc aaacgcgcac gcacgccgtc cagcagcgtt tgcatcgccg
    18121 ggccggaaag caggctcggc gtaccaccgc caataaagat tgtctttact tcacggccct
    18181 gagcgtaagc cacatcgttg tccaggtcgt taagcagatg ctgaacataa tcgtcgtgcg
    18241 gcacttctcc tttcaacgcg tgagagttga aatcgcagta cgggcatttc tgcacgcacc
    18301 acgggatgtg aatgtagaga cttagcggcg gtaatttaac cattacgtaa agcgtccagc
    18361 agcagtttca atgcctgacc acggtgggaa atggcgctct tttcttcgcg ggtcagttcg
    18421 gcagcggttt tcccttcgga aggtacgaag aagattggat cataaccaaa gccgccagta
    18481 cccgctggtt cacgagtaat catgcccggc cagctgccgt ggcacaccag cggagtggga
    18541 tcttccgcgt gacgcagata taccagtacg cagtggaaac gcgcctgacg ttggttgtcc
    18601 ggtacgtctt ttagtgtttc cagcagtttt tgcagattct tttgatcggt cgcgtcttca
    18661 ccggaataac gcgcggagta aatgcccggc gcgccgccaa gtacatctac cgccagacca
    18721 gagtcgtcgg caattgccgg taaaccggtc acttttgccg catggcgcgc tttcagaatc
    18781 gcgttttcga taaaggtcag gccggtttct tcagcggaat ctacgccgag gtctgtttgg
    18841 gccacgatat caagaccgaa gtcgctaagc agcgacgcca gctcacgcac tttaccggca
    18901 ttgccggttg cgaggacaac tttttgcata ggatacctaa ttaattaacg ccgcgatttc
    18961 tggcgggatt tgttgcggat taatgatttt aatttgtttg tggcggccaa gttcgccttt
    19021 ttcaatcacc acctggcttt tggcaacccg gaattgctta ccgagaaact tcaccagatg
    19081 gctgttggcc tgaccgtcaa ccggcggcgc ggtaatggcg actttaactt cgtcgccatg
    19141 taaaccgaca atagaatcac ggctggcttt cggctgaata taaagccgta aaaccagacc
    19201 gtcatcatta actgttacgg cactcataac gccatccaca gccccggcag cagcatgttt
    19261 ccggttgcct gtaatacttc tgcgacaccc atattgatga catacagcag cagaacgagg
    19321 atcatcggcg agaaatcaat cccacccatt gccggtagca ggcggcgaat cgggcgcagc
    19381 agcggatcgg ccagctgaat cagcacgtat tcaatcggac tacgaccctg gcttacccag
    19441 ctcataatcg ccatcaccag caggacccag aaaatcagca ggccgatggt tttcagcaga
    19501 atcagtaaac cggcaatcca gatgattggc aggaaggtca ccactttaaa cagcacgatg
    19561 gctttgataa aactgagaat gtacgcaacc agcagcgagg cgctatcaat gggacccatt
    19621 gccggaataa cgcggcgcag tggcccgata attggctgcg ttaccttcac tacaaactgt
    19681 gagaaggggt tgtaaaaatc acaatgagcc cactgcatcc agatgcgtaa taacagcacc
    19741 atggtataca gctcaatgac cgttgaaagc aggaaagtca acgtattcat ggcgttcctt
    19801 taattcctta ttttttagag taatcacgcg caccaaaaat tgcagtgccg atacgaacca
    19861 tcgtgctacc tgccgcaatg gcggcttcca tatcgtccga cattcccaga gagagcgtgt
    19921 cgatatgcgg gtagcgcgtt ttcagtccgg caaatgctac agccatttgg cgtgcaactt
    19981 caaactgcct tacatattct gactcaggcg cagggattgc catcagcccg cgcagacgta
    20041 aacgcggtag ttcagcgacc gccgctgcca gctcgtccag ttctgccagt tgaatcccgg
    20101 acttactgtt ttcatcacta atattaattt gaatcagtac gttaagaggg ggaagttctg
    20161 ccgggcgctg atcgttgagg cgggtagcaa tgcgcaaacg gtcgatggta tgacaccagt
    20221 cgaaatgctc tgccaccagg cggcttttat tagactgcaa cgggccaata aaatgccatt
    20281 ctaatcctgt tacgcccagt tcctgaaagt ggcgaatttt atctacccct tcctgaacgt
    20341 agttttcacc aaattgacgc tgcccggcat caatggcttc tgcgatggcg ctcgcaggtt
    20401 ttgttttact gactgcaagc agcgtaattt cttctggaga acggccgcaa cgcgttgcag
    20461 ccgctgagat tttgtcccgg acctgtgcca ggttatgcgc aatatcgttc attttccgag
    20521 gatcttagta tgaatatgga agaaattgtg gcccttagtg taaagcataa cgtctcggat
    20581 ctacacctgt gcagcgcctg gcccgcacga tggcgcattc gcgggctaat ggaagctgcg
    20641 ccgtttgatg cgccggacgt cgaagagcta ctgcgggagt ggctggatga cgatcagcgg
    20701 gcaatattgc tggaaaatgg ccagctggat tttgccgtgt cgctggcgga aaaccagcgg
    20761 ttgcgtggca gtgcgttcgc gcaacggcaa ggtatttctc tggcattacg gttgttacct
    20821 tcgcactgtc cacagctcga acagcttggc gcaccaacgg tattgccgga attactcaag
    20881 agcgagaatg gcctgattct ggtgacgggg gcgacgggga gcggcaaatc taccacgctg
    20941 gcggcgatgg ttggctatct caatcaacat gccgatgcgc atattctgac gctggaagat
    21001 cctgttgaat atatctatgc cagccagcga tgtttgatcc agcagcggga aattggtttg
    21061 cactgtatga cgttcgcatc gggattgcgg gccgcattgc gggaagatcc tgatgtgatt
    21121 ttgctcggag agctgcgtga cagcgagaca atccgtctgg cactgacggc ggcagaaacc
    21181 gggcatttgg tgctggcaac attacatacg cgtggtgccg cgcaggcagt tgagcgactg
    21241 gtggattcat ttccggcgca ggaaaaagat cccgtacgta atcaactggc aggtagttta
    21301 cgggccgtgt tgtcacaaaa gctggaagtg gataaacagg aaggacgcgt ggcgctgttt
    21361 gaattattga ttaacactcc cgcggtgggg aatttgattc gcgaagggaa aacccaccag
    21421 ttaccgcatg ttattcaaac cgggcagcag gtggggatgt taacgtttca gcagagttat
    21481 cagcagcggg tgggggaagg gcgtttgtga cgttgccatg cccgatgcgc aagtttat
//
LOCUS       DAZFVO020000085        21273 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFVO020000085 DAZFVO020000000
VERSION     DAZFVO020000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.085"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            250..750
                     /gene="tssB"
                     /locus_tag="ACHKNB_RS14570"
                     /old_locus_tag="pgaptmp_002914"
     CDS             250..750
                     /gene="tssB"
                     /locus_tag="ACHKNB_RS14570"
                     /old_locus_tag="pgaptmp_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4196389.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            785..1009
                     /locus_tag="ACHKNB_RS14575"
                     /old_locus_tag="pgaptmp_002915"
     CDS             785..1009
                     /locus_tag="ACHKNB_RS14575"
                     /old_locus_tag="pgaptmp_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196390.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1060..2535
                     /gene="tssC"
                     /locus_tag="ACHKNB_RS14580"
                     /old_locus_tag="pgaptmp_002916"
     CDS             1060..2535
                     /gene="tssC"
                     /locus_tag="ACHKNB_RS14580"
                     /old_locus_tag="pgaptmp_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4196391.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2542..2955
                     /gene="tssE"
                     /locus_tag="ACHKNB_RS14585"
                     /old_locus_tag="pgaptmp_002917"
     CDS             2542..2955
                     /gene="tssE"
                     /locus_tag="ACHKNB_RS14585"
                     /old_locus_tag="pgaptmp_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HGU4196392.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2959..4809
                     /gene="tssF"
                     /locus_tag="ACHKNB_RS14590"
                     /old_locus_tag="pgaptmp_002918"
     CDS             2959..4809
                     /gene="tssF"
                     /locus_tag="ACHKNB_RS14590"
                     /old_locus_tag="pgaptmp_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HGU4196393.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4773..5855
                     /gene="tssG"
                     /locus_tag="ACHKNB_RS14595"
                     /old_locus_tag="pgaptmp_002919"
     CDS             4773..5855
                     /gene="tssG"
                     /locus_tag="ACHKNB_RS14595"
                     /old_locus_tag="pgaptmp_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HGU4196394.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5880..7160
                     /gene="tagH"
                     /locus_tag="ACHKNB_RS14600"
                     /old_locus_tag="pgaptmp_002920"
     CDS             5880..7160
                     /gene="tagH"
                     /locus_tag="ACHKNB_RS14600"
                     /old_locus_tag="pgaptmp_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HGU4196395.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFMQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7157..7681
                     /gene="tssJ"
                     /locus_tag="ACHKNB_RS14605"
                     /old_locus_tag="pgaptmp_002921"
     CDS             7157..7681
                     /gene="tssJ"
                     /locus_tag="ACHKNB_RS14605"
                     /old_locus_tag="pgaptmp_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HGU4196396.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7684..9015
                     /gene="tssK"
                     /locus_tag="ACHKNB_RS14610"
                     /old_locus_tag="pgaptmp_002922"
     CDS             7684..9015
                     /gene="tssK"
                     /locus_tag="ACHKNB_RS14610"
                     /old_locus_tag="pgaptmp_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4196397.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9020..9781
                     /gene="icmH"
                     /locus_tag="ACHKNB_RS14615"
                     /old_locus_tag="pgaptmp_002923"
     CDS             9020..9781
                     /gene="icmH"
                     /locus_tag="ACHKNB_RS14615"
                     /old_locus_tag="pgaptmp_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HGU4196398.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9790..12555
                     /gene="tssH"
                     /locus_tag="ACHKNB_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /gene_synonym="clpV"
     CDS             9790..12555
                     /gene="tssH"
                     /locus_tag="ACHKNB_RS14620"
                     /old_locus_tag="pgaptmp_002924"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4196399.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12552..13295
                     /gene="vasI"
                     /locus_tag="ACHKNB_RS14625"
                     /old_locus_tag="pgaptmp_002925"
     CDS             12552..13295
                     /gene="vasI"
                     /locus_tag="ACHKNB_RS14625"
                     /old_locus_tag="pgaptmp_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HGU4196400.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13300..14712
                     /gene="tssA"
                     /locus_tag="ACHKNB_RS14630"
                     /old_locus_tag="pgaptmp_002926"
     CDS             13300..14712
                     /gene="tssA"
                     /locus_tag="ACHKNB_RS14630"
                     /old_locus_tag="pgaptmp_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HGU4196401.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14821..18255
                     /gene="tssM"
                     /locus_tag="ACHKNB_RS14635"
                     /old_locus_tag="pgaptmp_002927"
     CDS             14821..18255
                     /gene="tssM"
                     /locus_tag="ACHKNB_RS14635"
                     /old_locus_tag="pgaptmp_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HGU4196402.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18266..19715
                     /locus_tag="ACHKNB_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /pseudo
     CDS             18266..19715
                     /locus_tag="ACHKNB_RS14640"
                     /old_locus_tag="pgaptmp_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19642..20124
                     /locus_tag="ACHKNB_RS14645"
                     /old_locus_tag="pgaptmp_002929"
     CDS             19642..20124
                     /locus_tag="ACHKNB_RS14645"
                     /old_locus_tag="pgaptmp_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4196403.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20168..21082
                     /locus_tag="ACHKNB_RS14650"
                     /old_locus_tag="pgaptmp_002930"
     CDS             20168..21082
                     /locus_tag="ACHKNB_RS14650"
                     /old_locus_tag="pgaptmp_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196404.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLTSFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21092..>21273
                     /gene="ykfM"
                     /locus_tag="ACHKNB_RS14655"
                     /old_locus_tag="pgaptmp_002931"
     CDS             21092..>21273
                     /gene="ykfM"
                     /locus_tag="ACHKNB_RS14655"
                     /old_locus_tag="pgaptmp_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4196405.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMS"
BASE COUNT         5097 a         5725 c         5795 g         4656 t
ORIGIN      
        1 aaattattgc cataatacac ctgacgcgaa ttgcacaggt tcacacaatc tatttaacgc
       61 ttgcagaata cccctcttta cgatcgataa aaagtccttt tcttatttat tttgaagcgt
      121 aaaatgcggg gtgatgaagg agtggtgatt ttattttcac cgccattagg gcggcattgc
      181 cgatattttt ttgcggtaca aatatgccgt gttcatggcc tgtacgagat aacatgacgg
      241 atcactttta tgagcaaaaa atttgaaggc agcgtcgcgc ctcgcgagcg tattaatata
      301 tcgtatgtcc caaaaacaga tggccagacg gctgaagtgg aacttccgct taacatgctc
      361 gttgtcggcg ataccggaaa tacgcaggaa acatccccac tggatgaacg tcaggcagtt
      421 tccgttaaca agcacaattt cggtgcagtg atggctgaag cagccattgg cctcaatttt
      481 actgtccccg ccacgctgaa gggaagcaca acagacgatg aactgaatgt ggcgctgaat
      541 atcaaatcac tggatgattt ttcaccagat agcgtggccc ggcaggttcc ggaagtgaat
      601 aaattgctgg aactgcgtga agcactgact gcactgaagg ggccgatggg taaccttccg
      661 gcattccgta cccagctcca ggcattgctg gaaaatgaag agtcccgcga gcagttgctc
      721 aaagaaatcg gtctggtgag caataaataa ccaccactat ttcattaaac agggttatcc
      781 aggcatgacg gcacacaaaa atatctccgg gacataccac ctctctgttg cagacattct
      841 gcaggtggtt taccaggtat gtttcagccc gtccgttgaa atcaaccagg atggtgtggc
      901 tgcgctgatt acaacactgg atcgccgtat cagtgatcta ttggatgaga ttatccattt
      961 ttgtgagttt cagcagagcg cgtcgcactg gcagcgcgta cttcactgac ggcgaaaagc
     1021 cggtttcaga aacacagcta aaacagaagg attttcagta tgtctttaca ggaagaggaa
     1081 ctcgtatcca gtcatgccgg gcagcctgag caggcatcgt cgctgcttga tcaaatcatg
     1141 gcacagaccc gtattcagcc cggttcggaa ggttatgacg ttgcccgcca gggtgtgaca
     1201 gcctttattg ccagcatcct gcaatccact gcatctgccg aaccggtcaa taaactggcc
     1261 gtggacagca tgattgcaga tattgatgaa cgcatcagcc gccagatgga tgtcatcatc
     1321 catgcgcctg cctttcagca ggtcgagtcg ttctggcgct cactgaaaac catggtggat
     1381 cgcgttgatt tccgtgaaaa catcaaggtc aacgtgcttc atgtcaccaa gcaggaactt
     1441 ctggaagact ttgagtttgc gccagaaatt attcagtccg gattttataa gcacgtttat
     1501 tcgtccggtt tcggacagtt tggcggtgaa cccatcgctg ccgtgctggg cgcttatgag
     1561 ttcaaaaata ccgcgcctga catgaaactg ctgcaatacg tcagtgccgt gggcgcaatg
     1621 gcgcacgcac cgttcctgtc ctccgtttcc ccggagttta tggggctgaa ctcgtggacc
     1681 gaactgccta atatcaaaga tctttatgcc atctttgaag gtccggccta caccaaatgg
     1741 cgcgccctgc gtgactcgga agattcgcgc tatttagggc ttacggcacc ccgcttcctg
     1801 cttcgccagc cctattcacc aacagataac cctgttaaga actttaacta ttacgaagat
     1861 gtcagccaga accacgaaga ttatctgtgg ggtaatacgg cctggatgct ggcatgcaat
     1921 atcgcagaca gttttgccaa ataccgctgg tgtccaaaca ttatcggtcc gcaaagcggc
     1981 ggcgcagtga aagatctgcc ggtgcatctg ttcgaaacga tggggcagat tcaggccaaa
     2041 atcccaaccg aggtgctggt caccgatcgc cgcgaatttg aactggctga agagggtttt
     2101 atcaccctta ccatgcgtaa agactctgat aatgcagcct ttttctctgc aaactcggta
     2161 caaaaaccga agcactttcc gggaaaagat gcagaaacca actataagct gggtacgcag
     2221 cttccttatc tcttcatcat caatcggtta gcgcactaca tcaaagtgtt gcagcgtgaa
     2281 caactggggt catggaaaga aaggagcgac ttagaaagag agcttaatac ctggatccgt
     2341 cagtatgttg ccgaccagga aaacccgcct gcagacgtgc gcagccgcaa acctctgcgc
     2401 gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg gctggtacca ggttgcgcta
     2461 agcgtgaggc ctcattttaa atttatgggg gcaaattttg agctttccct ggttggccgg
     2521 ttagacaggg agtaacacac catgataccg gatcgggaaa gaaccacagg ttctcttttt
     2581 gagcgtatgg aagcctcttc ggccagaaac cggcagggag gcagtattca ttcgctcagg
     2641 caatccattc gccagaacct acgaaacatt cttaataccc ggtccggtag ttgccggggt
     2701 gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa acttccgcga atccatgagt
     2761 cgggccatcg agcaatgtat agaacgctat gaaccccgga tctcacacgc tgaagtgcag
     2821 gcagtggttt catccgcctc cagcccgctg gatatgacgt ttcatattac agcctgggtg
     2881 acatttaacg aaacgcatga ggtgcttgaa tttgatatgg caccgaatgg cagccagcat
     2941 taccgggtgg actgacaaat ggaatttgaa gaacgctatt tccgggaaga actcgattac
     3001 ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc atctggcccg cttcctggcc
     3061 gaaaaagatg cggatccgga tattgaacgc ctgctggaag gggtggcttt tcttaccggc
     3121 aatctccgcc agaaaattga ggatgaattc ccagaactga cgcacgggct tattaagatg
     3181 ctatggccta attacctgcg tccggttccg gcaatgaccc ttattgaata tacgccggat
     3241 atggataagt cttctgtacc ggtgttaatc ccccgtaatg agcagtttac aaccaacgcc
     3301 ggggaaatca gagttgatga agtgctgccc tctgatgcta aaaaggagga gccgcctccc
     3361 tgtaccttca ctctctgccg ggatatctgg ctgctgcccg ttcgcctgga gcagattgaa
     3421 aaccgcagta cgacccgtaa tggtgttatc aacatcacct tttcggtcgc accgggaacg
     3481 gacttccgca cgctggatct gaacaaactt cgcttctggc tcggcaatga cgacaactat
     3541 acccgtgacc agctttattt atggttctgc gaatacttgc agggtgccga cctgactgtg
     3601 ggtgaacagc atattcgcct gcctgagttt atgctaaaag ctgtcggttt tgagccgcag
     3661 gatgccatgc tgccctggcc gaaaaacgtc cacagcggct accggatcct tcaggagtat
     3721 ttctgttacc ccgatgcgtt tctctttttt gatctttgtg gttgtccggc tttgcctgac
     3781 ggattgcagg cggaattctt taccctgcaa ctgcgttttt cgcgcccttt gcccgtggac
     3841 atccggctgc gccgcgattc cctgcgcctg tattgcgcac ctgccattaa tttatttatc
     3901 caccatgcag aagccatcac gctggacaac cggcgggcag actatccgct ggttcccagc
     3961 cgccattacc cacaacatta cgatgtattt tccgttaaca gtgtggtgag ccaggtccag
     4021 gatatgttca ggaaaaaaga tctggggcgt cctgtttcga cgcaggccgc gcgccagtgg
     4081 ccagcctttg aaagtttcag ccatcagatg gaatacagcc ggaagcggga agtggtgtac
     4141 tggcatcacc ggaccaaaac atccctgttc catcgcggct ttgatcatac ccttgccttt
     4201 atacatgctg atggcagtta tccgtcagac gaatctctgc tcagtaatga agtggtttcg
     4261 gtatcgctga cctgtaccaa ccgtgagctt ccgtcacaaa ttcgttccgg cgatatcacc
     4321 ggcacaaccg gtaaaaatgc ggctgttgct tcatttcgca acattacccg cccgacgcaa
     4381 ccactctggc cggtcattga tggcagcctg cactggtccc tactctccgc catgaacctg
     4441 aattatctgt cattactgga tacggacgcg ctgaagcagg tcatcgccaa ctttgatcgc
     4501 cacgcaatcc atcatccgca gacggcacgg ctgtcacaac aaaagctgga tgccattgag
     4561 cgtctggaga cccgccccgt tgatcgcctg tttacgggta ttcccgtccg gggactggcc
     4621 tccacgctgt atctgcaccc ggagccgttt gtctgtgaag gggaaatgta tctgctcggt
     4681 acggtgcttt cgcattttct gtcgctgtac gccagcgtta actcattcca catgctgacc
     4741 gttgtgaaca cagaaagcca ggagacatgg aaatggacgg aaagaatcgg gcagcatcct
     4801 cttatctgag tccgggaaat cccccggcag ataaagagca gaatgatccg ctggcacagg
     4861 ttttccataa tgcctgctct tacaattttt ttgcgatggc ggagctgttg caccgcctgg
     4921 caaagggtga aaaggggacg ccagaattat ccctgcgtga cgatccggca caggaaaccc
     4981 tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag tgatatcagt gcactgaaac
     5041 gggatacgtc aggggcattc aggatgacca ccacctttat ggggttacag gggagccagt
     5101 cgcctctccc cggttattac ctcgatcacc tggcctggaa agcggtacat gaacaaagtc
     5161 cggtaggtga ttttctggat atgttcagcc accgcctgac ccagtttgtc tggcatatct
     5221 ggcgtaagta ccgctatcac atcagttttc gtaacggcgg tgtggatgcg ttctcgcagc
     5281 gcatgtactc ccttgtcggg ctgggccacc gccagcttcg cgataagctg gcgattaatc
     5341 acagcaaaat gctggcgtat tccgggatcc tggcaaatcc gggacgatcc ccggaaataa
     5401 tctgtggcct ggtgtcccac tgctttgatt tgtcagaagt gacgctgcaa aactggcaac
     5461 gtcgcaaggt ggatattgaa ccggatcagc aaaatagtct ggggagctat tctcttaaaa
     5521 atggcgaaaa actggcggga agatcggtac tgggcaactt tgttctgggt acacgagtgc
     5581 ccgatctcag cggaaaattc cagctcagta ttaccagcct gacccgaaaa cagttcctct
     5641 cttttctgcc gtccggcgaa aacttcctgc cactgacgat gttcgtgtcc ttcatcctgc
     5701 gcgatcagct tgcctgggac ttacatctgg ggctggcgcc ggagcaggtg ggcgcaatgc
     5761 gcctgggcga taacaaaagt gcgctgctgg gctggaccag cttcctcggc acaccggaag
     5821 aacgcccctc agtcaccatc agggtacggt cgtaaattat gataatcacg gagcagaata
     5881 tgccggaaga gaaactacag acgctttcat tgcaggtcat taacggcagt gagctggaaa
     5941 gcggacgggc ggcgcgctgt ctgttcacac agcagggaaa tgtcggccat ggccccgaat
     6001 gccactggtc ggtacaggat cgtcagcaga gcattccggc ccaggctttt accgttatcc
     6061 tgcacgatgg cacattttgt ctgcgcccgc agacggcaca actgtggctg aatcaggcaa
     6121 aagtcacagc aacatcagac ctaatacagt tgcgccaggg cgatgagatc cagatcggac
     6181 ggctgatggt gagggttcat ctgaaccggg gagatattcc ccattacgat gaggaaatgg
     6241 ccactcccga aaccatcgtt accaatcgcg atatgctcac ggataccctg ctatcaacgg
     6301 agggtacgcc acactatccg ggaatgactc accggcacca acttgcagac accgtggtaa
     6361 acggtttttc tgccgatcca ctccaggcac ttcagtccga aagcctgatt accacgggcg
     6421 atccgctttc aggcattgcg gctgtccggc catcggcacc gctgtccgat ccggcaagta
     6481 atggggggat caatactccg tttatggatc tgccgcccat ttatgccagc cccggcgatc
     6541 gtaatgacga cgtctctgcg gcagaaatgg cgcaacgcca ccttgcggtc acccccttac
     6601 tgcgcggtct tggcggctcg cttaccatga gcaattccga cgatgcggat gattttctgg
     6661 aggaggccgg acgaacgtta caggccgcaa taaaaggtct gctcgatttg cagcagcagc
     6721 gtaacagcct ctcagacaaa catttgcgcc cgctggaaga taacccgctg cgcctgaaca
     6781 tggattacgc caccgcgctc gacgtaatgt ttgccgaagg taaaagcccg gtacatttgg
     6841 cggctcccgc cgccgtcagt gaaagcctgc gcaatgtccg ccaccacgaa gaagctaacc
     6901 gggcagcgat tgtggagtcg cttcgtgtcc tgctggatgc tttctcacca caaaatctgc
     6961 tgcgccgctt tatgcagtac cgccgcagcc atgaactgcg ccagccgctg gatgatgccg
     7021 gagcatggca aatgtacagc cattattacg aagaactggc ctccgatcgc cagcaggggt
     7081 ttgagatgct gtttaacgag gtctacgccc aggtctatga ccgggtgctt cgtgaaaaac
     7141 agcgggagcc ggaagcatga acaggaaggc gtttctggcc tgcgtgctga tgtgcgtcat
     7201 attaaccggc tgcgaaacgg cgaaaaaaat cagtgaggtg atcaaaaacc cggatatcca
     7261 ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc accctgctga ccgaaccgga
     7321 caccaatacc aatgcggaag gtgaatcagc cgccgtggat gtgcaactgg tgtacctgac
     7381 tgacgactca aaattgctgg cagccgacta tgaccagata gccagtacgc cgctaccgga
     7441 tgtgctgggt aaaaactata tcgaccacca ggatttcaac ctgttgccgg acaccatcaa
     7501 aaccctgccg ccggtaaagc tggatgagaa aacgcagttt attggtgtcg tggcctattt
     7561 ctccgatgac caggccactg aatggaaaca aatcgagacg gtggaaggaa ccggccacca
     7621 ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa atgaaaaaag aggatgaata
     7681 attatggcaa ccacccgcaa caaggtgatg tggcaggaag ggatgctgat gcgtccacac
     7741 catttccagc agcagcagcg ttacaacgac tacctggata accagcgttt ccgggccatg
     7801 aatgatttat cctggggatt taccgaactc actctcaaca atgaactgct ggcacagggt
     7861 aagatcatga ttgacagcgc gtcaggcaca ctgcccgacg gcaccgtctt ttctatcccc
     7921 gaccaggacg cactgcccga tccgctgcac ccgcaacatt ttccggacga gagaagccgc
     7981 aatatctacc tcgctctgcc ggtcgccagt gatgtgagaa atgaaatcag cgacgggcgg
     8041 cgaatcgggc gttaccggct gaattatgcc gatgtccggg atttgcattc agaagaaggt
     8101 gacacgcgaa cactgacgct gggacaactg acgccgcgca ttatgagcgg tgcagaagat
     8161 atgagcgcct atattacgct cccgctttgc cgtatcagcg atcgccatgc cgacggctcc
     8221 ctgacgctgg atgacgattt tatcccctcc tgccagaata ttcaggtcag taagaaactg
     8281 cgtgtttatc tcaaagaggt acagggggcc attggcggac gggcaagcga tctggcaaac
     8341 cgcattggct ctccggcgca gagcggcatc gcggatgtgg cggaatttat gatgttgcag
     8401 ttacttaacc gtaaccagac ccggtttacc catcacgccc gtcgatccca gctccacccg
     8461 gaagatttct accttgatct tgccgggttg ctgggtgaac tgatgacctt taccgagccg
     8521 tcgcgcctgc cctgcccgct tgatgtgtat gatcatcatg acctgaccaa aacatttaaa
     8581 acgctgttac cggaagtcaa acgggcgctg cataccgtac tgtcgccaag agccgtcaat
     8641 ctgccgctgc atctgcgtga cggtatctgg caggccgata tccatgacac agaactgctg
     8701 caatctgcca cctttgtgct ggccgtggcg gcaaacatgc cggtcgatca gatccagcgc
     8761 cagtttatcc agcagtcgaa aatttcctcg ccggaaaaaa tccgcaatat ggtcagtgtg
     8821 cagatccccg gcattccatt gcgcgcactg atggtggctc cccgccagct tccttaccat
     8881 tccgggttca gctatttcga actcgacaag agcggacagg cctggacaga aatggctgcc
     8941 gccggggccg ttgcactgca tgtatccggc agtttcccgg atctgaacat gcaactgtgg
     9001 gcgataagag ggtaagacga tggatgaggg gtcactgtct ctgccaccat ttaccggata
     9061 cgacgaaaaa agccagcgca actaccatct cgcgcttcgt ggcaacagcc tgaatccgat
     9121 gatcgacgcg gccacgccgc tactcggtat ggtaatgcgc ctttcaacca tgaacagcca
     9181 gacgatgccg gagcatcttt ttgctcaggt ggtgacggat gttcaggccg tggagcaact
     9241 gcttcaggag cagggctacg aaccgggcgt gattatctcg tttcgctaca tcctctgcac
     9301 gtttattgat gaagcggcgc tcggtaacgg ctggtcaaac aagaacgagt ggatcaagca
     9361 gtcattgctg gtccatttcc acaacgaagc ctggggtgga gagaaagttt tcatcctgct
     9421 ggagcgcctg atccgtgaac ccaaacgtta ccaggatctg ctggaatttc tctggatctg
     9481 tttttctctg ggatttcgcg ggcgctacaa agtggcagca caggatcagg gggaatttga
     9541 gcagatctac cgccgtctgt accacgttct gcataaactg cgcggcgatg cccctttccc
     9601 gctgttacac caggacaaaa aaacccaggg cgggcgttat cagctcatca gtcgcctgac
     9661 tgttaaacat attttttgtg gtggcgttgt cgtgctggcg ttgttttacc tgttctatct
     9721 gttgcggctg gacagccaga cgcaggacat cctgcaccag ctaaacaaat tacttcgata
     9781 aggacgttta tgatccagat tgatcttccc acgctggtaa aacggctgaa cctgttctcc
     9841 cgccaggcgc tggagatggc cgcctctgaa tgtatgagtc agcaggcagc ggaaattacc
     9901 gtcagccatg tgctcattca gatgctcgcc atgccacgca gtgacctgcg ggttattacc
     9961 cgccagggcg atattggcat ggaagagttg cgccaggcgc tgacggtaga gaactacaca
    10021 accgcccgtt ctgcggacag ctacccggcg ttttccccga tgctggttga gtggctgaaa
    10081 gagggctggc tgctggcgtc ggctgagatg cagcacagcg aactgcgcgg cggcgtgttg
    10141 ctgctggccc tgctgcattc gccgctgcgt tatataccgc ctgctgccgc ccggctgttg
    10201 accggcatta accgtgaccg tctgcaacag gactttgtgc agtggacaca ggagtcggcg
    10261 gaatcagtcg tgccggatgc agacggtaaa ggcgcaggca caatgacgga cgcctctgac
    10321 accctgcttg cccgctatgc caaaaacatg accgcagacg cccgtaacgg caggcttgac
    10381 ccggtactgt gccgcgacca cgaaatcgac ctgatgatcg acattctctg ccgccgccgt
    10441 aaaaacaacc cggtggtggt gggcgaagcg ggcgtgggca aaagcgcact gattgaaggg
    10501 ctggcgctgc gcatcgtggc aggccaggtg ccggacaagc tgaaaaacac cgatatcatg
    10561 acccttgacc tgggcgcatt gcaggccggg gcgtcggtga agggtgaatt cgaaaaacgt
    10621 ttcaaagggc tgatggcgga ggtcatttcc tccccggtgc cggtcattct gtttatcgac
    10681 gaagcacata ccctgattgg cgcgggcaac cagcagggcg ggctggatat ctccaacctg
    10741 ctcaaaccgg cgctggcgcg cggcgagctg aaaaccatcg ccgccaccac ctggagcgag
    10801 tacaaaaaat acttcgaaaa agatgccgcc ctgtcgcgcc gcttccagtt ggtgaaggtc
    10861 agcgaaccca acgctgccga agccaccatt attctgcgcg gtctgtcggc ggtctatgaa
    10921 cagtctcacg gcgtgctgat tgatgatgac gccttgcagg ccgctgcgac attaagcgag
    10981 cgttatctct ccgggcgtca gttaccggac aaagcgattg atgtgctgga taccgcctgc
    11041 gcccgtgtgg ccatcaacct gtcgtcgccg ccgaagcaaa tctcggcgct gaccactctg
    11101 agccaccagc aggaggcgga aattcgccag cttgagcgcg agcttcgcat cggactgcgt
    11161 accgacacat cacggatgac cgaggtgctg gtgcagtatg atgaaacgct gacggcgctg
    11221 gatgaactgg aagcggcctg gcaccagcag cagacgctgg tccgggagat tattgcgctg
    11281 cgccagcagt tactgggcgt ggcagaggac gatgcggcgc cgttgccgga cgcagatacc
    11341 gtggaggata cgcagccaga gtcagagtca gaacaggata ataccggtgc cgtaccggct
    11401 gatgaggccg acagagaaca gccggaagag accgctgaaa cagtttcccc ggtacaacgt
    11461 ctggcacatc tcactgccga actggacgcc ctgcataacg accggttgct ggtttccccg
    11521 cacgtcgata aaaaacagat tgcggcggtg attgccgaat ggaccggcgt gccgcttaac
    11581 cgcctgtcgc agaatgaaat gtcggtcatc accgacctgc caaaatggct cggcgacacc
    11641 atcaaaggcc aggacctggc gattgccagc ctgcacaaac acctgctgac cgcacgcgcc
    11701 gacctgcgcc gtccgggacg cccgctgggc gcgttcctgc tggctggccc cagcggcgtg
    11761 ggtaaaactg aaaccgtcct gcaactggca gagctgctct acggcggtcg ccagtacctg
    11821 accaccatca atatgtccga gttccaggag aaacataccg tctcgcgact gattggttcg
    11881 cctccgggct acgttggcta cggtgaaggc ggcgtactga ccgaagcgat tcgccagaaa
    11941 ccctactcgg tagtactgct cgatgaagtg gaaaaagcgc acccggatgt gctcaacctg
    12001 ttctaccagg cgttcgataa gggcgaaatg gcagacggtg aaggccgcct gattgactgc
    12061 aaaaatatcg tcttcttcct gacgtccaac ctcggctacc aggtaatagt cgagcatgcc
    12121 gatgacccgg aaaccatgca ggaagtactg tatccggtgc tggccgactt cttcaaacct
    12181 gccctgctgg cgcgtatgga agtggtgccg tacctgccgc tgtcgaaaga gacgctcgcc
    12241 accatcattg ccgggaaact ggcccgcctg gataacgtgc tgcgcagccg ctttggcgcg
    12301 gaggtgatta tagaaccgga agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa
    12361 aacggcgcga ggatgctgga atcggtcatc gacggcaata tgctgccgcc gctctccctg
    12421 ctgctgttgc agaaaatggc ggcgaatacg gcggttgccc ggattcggtt gtcggcagtg
    12481 gacggcgcat ttacggcaga cgtggaagat gctcagaacg acgagtccgt cacaaaggat
    12541 gaaacggttt tatgagcagg ttgcgcgttt tccggcagac aggatggttt atcgcgggcc
    12601 tgatgacggg cttacccgcc actgccgcgc ccgcagaagc atcgtcaatg gccggtgtgg
    12661 cggtcgccgt cgccaccacg accccgccgg atgcgacagc cacgttacag gccatgcagt
    12721 cctgtcggcg ggagtccgcg gcgctggagc ggcttgactg ctatgaccgc ctgctggcac
    12781 cgctgtcccc gtcagggttt gacggggcgc tggtcaaagc cggttttgtg ggcgaagcct
    12841 ggacccgcgc cacggaacag gaaaaacacc gccagggcaa caccacagag ctgctggtga
    12901 cgcaggtgcc gggggagcgc ccgacggtgg tgataaccac cccggctatc ggtcatgttc
    12961 cgccacgccc ggtgctgatg ttcagttgcg tggacaatat cacccggatg caggtggcgc
    13021 tgatgcaccc tctggatgtc catgatatcg ccgtcactct aaatgccgac aaccgggcat
    13081 tgcgcagcca ctggtttgtg cgcgagaacg gcaccctgct ggagtccagc cgggggttat
    13141 ccgggattga tgaaattaag caactgttcg gcgcaaaaac gctgacggtg gataccggcg
    13201 cagacaatgc cgccggaaag ctgaccttta acattgacgg gctggcaagg gcgattgctc
    13261 ccctgcgcga tgcctgccac tgggcgggag aataaaaata tggcaatgga cttacgcgat
    13321 ccgaatgtct ggatatcgca cctgctggaa aacctgccgg aagagaaact ggcatcggcg
    13381 ctgaaagatg acaacccgaa ctgggagtat atcgacggcg aaatcgtcaa gctgggatcg
    13441 ctggcccaca gtcaactgga tatcccggaa ctccagcgcc gggggctggt cattctggct
    13501 tcggagagta aggattttcg tctgctggcg catctgctga gaaccctgca acacgccggt
    13561 gatcctcatc tcgcattgcg cctgctggcg ctgtatgtgg agcattactg ggcggtggct
    13621 gcgccgcaga atatggcgca taaaaaacgc tttgccagcc aggtcattaa acgctttgaa
    13681 acaggtattg aagttttttc acagaacgcc gccacagcgc agcgcgatgc gctgtcaggg
    13741 gaactggcaa aactggcgca atgctggcag tcacataacg ccccggaact ggcacaggct
    13801 actgatgatc tttttgccct gtaccagcgt gcgtttaatc gtgcggcacc ggctcctgtc
    13861 cccactccgg cggcctccgg tagttcacca cagaccaccg ccacgtctga aagcggcgtg
    13921 acgcaaccca gtgctccggc tccccaaatc gtcatcgaca gtcacgacga caaagcctgg
    13981 cgcgacacgc tgttaaaagt ggcggccatt ttatgtgaac gccagccgga ttcaccgcag
    14041 ggctaccgtc tgcgccgcca tgcgctttgg cagaacatca ccagtacgcc ccaggcggaa
    14101 agcgatggcc gtactccact ggctgcggtc tccgccgata tggtggcaga ttaccatgct
    14161 cagctcggta gtgccgatat ggcgctgtgg cagcaggtgg agaaaagcgt actgctggct
    14221 ccttactggc tggatggtca ctgcctttct gcacagacgg cgctgcgtct gggttacaaa
    14281 caggtggcag acgccatccg cgatgaggtc atccgcttcc ttgagcgtct gccccagctt
    14341 accgggctgc tgtttaatga ccacacaccg tttatcagtg agcagacgaa acaatggctg
    14401 gcagcttcgc ccgacgccaa agttgcaccg gttgcgcaaa tcggtgagga atcgaaggca
    14461 gccagagcct gttttgctga gcagggtctg gaggcggcgc tgcgatatct ggacatgcta
    14521 cccgaaggcg atccccgcga tcagtttcac cgccagtacc ttgccgcaca gttgacggaa
    14581 gaggcggggc tggtacagct tgcgcagcag cagtaccgga tgttgttcag gatgggattg
    14641 cagatgatgg tggcagactg ggagccgtcc ctgcttgagc agctagaaca aaaattcacg
    14701 gcagaacaat aaaagagaac aggagttttt gtgttcagat taccaacacc ccgactactc
    14761 agcggactca aatcagccct gcgaccggcg atgccccggt ttaaagtctc tgctttctgg
    14821 ctgctgatac tggcgtggat ttttctgctg gtgtggatct ggtggaaagg cccgacgtgg
    14881 acgctgtatg aagaacagtg gctcaaacca ctggcgaacc gctggctggc aacggcggcg
    14941 tgggggatta ttgccctgat gtggcttacc gtccgggtga tgaagcgcct gcaacagctt
    15001 gaaaaaatgc aaaagcaaca gcgcgaggaa gccgttgatc cgctcagcgt ggaactgaac
    15061 gcccagcagc gttatcttga ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc
    15121 cgtttcctgt ggcagttgcc gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg
    15181 acactgctgc gcgaggggtt tccgtccgac attatttatg ccccggaggg cgcacgcggc
    15241 gcagaacaac gcctgtacct cacgccccat gtcggtaaac aggcggtgat ctttgatatc
    15301 gacggcacac tctgcgctcc cgctgatgcg gatatcctgc atcgccgtct gtgggaacat
    15361 gccctcggct ggctgaaaga aaagcgcgcg cgccagccgc tgaatgggat tattctgaca
    15421 ctcgatttac ccgatctgct taccgcagac aaacgccgcc gcgagcatct gttacaggcg
    15481 ctgcgcagcc gcttgcagga tatacgccag catcttcact gccagttacc ggtttacgtg
    15541 gtacttaccc ggcttgattt attgcagggt tttgccgccc tgtttcagtc cctgaacaga
    15601 caggatcgcg atgcgattct gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac
    15661 tggcgaacag agttgaatgc tttctggcag acatgggtgg atcgaatgaa tctggcgttg
    15721 ccggatctga tggtcgctca gactcacacc cgcgcgtctt tattcagttt ttcccgccag
    15781 atgcagggaa gccgtgaacc gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat
    15841 atgaacgtga tgctgcgtgg tgtctatctc acctcttcgc ttcagcgtgg acagatggat
    15901 gatatattca cccagtctgc cgcccgccag taccggctgg gcaataaccc actggcgtcc
    15961 tggcccctgg tggacaccgc gccttatttc acccgcagcc tgttcccgca ggcattactc
    16021 gcagagccta atctggcaac agagagccgc gcctggctga tacgttcccg tcgccgcctg
    16081 acggttttct ctgccacagg cggcgtggca gcactgctgc tcatcaccgg ctggcatcac
    16141 tattacaacg gtaactatca gtctggcatc accgtgctta agcaggccaa agcctttatg
    16201 gacgtgccgc ctccgcaggg ggaagatgac tttggcaacc tgcaactgcc gctcctgaac
    16261 ccggtacgcg atgccacact ggcctatggc gactggggcg accgcagccg tctggccgat
    16321 atgggactgt accaaggacg acgtatcggg ccttatgtgg aacagaccta tctgcaactg
    16381 ctggagcaac gttacctgcc ctcgctgttt aacgggctgg tcaaagagct gaacgccgcg
    16441 ccgccggaga gtgaagaaaa actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa
    16501 agcggacgta acaatcaggt ggtgaagcag tatatggcaa aacgctggag cgaaaagttc
    16561 cacggtcagc gcgatatcca ggcacaactg atgtcccatc ttgactacgc gctggctcat
    16621 actgactggc acgcagagcg tcaggcgggc gacggtgacg ccatcagccg ctggacgcca
    16681 tatgacaagc ccgtggtatc agcacagaaa gaactgagca aactgcctgt ctaccagcgg
    16741 gtttaccaga gcctgaaaac gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt
    16801 gaccaggtag ggccaacctt tgaccaggtg tttacatctg ccgatgacaa caaactggtt
    16861 gttccacagt ttcttacccg ttacggcctg caaagctatt ttgtaaaaca gcgcgatgaa
    16921 ctggttgaac tgacggcgat ggattcctgg gtacttaacc ttacccgcag cgtgaaatac
    16981 agtgacgccg accgcgcgga aatccagcgc cagttgaccg agcagtatat cagcgactac
    17041 accgccacct ggcgggccgg gatggacaat ctgaatatcc gcaattttga gtccatcgga
    17101 caactgaccg gggcgctgga gcaggttatc agcggcgacc agcctttgca gcgggcgcta
    17161 accgtgctgc gtgacaacac acagccaggc gtcttttctg aaaaactctc tgccaaagaa
    17221 cgggaggaag ccctggcaga gccggattac cagttactca cccgcctcgg gcatgaattc
    17281 gccccggaaa acagtaccct ggcagtacag aaagacaaag aaagcacgat gcaggccgtg
    17341 tatcagcaac tcaccgagtt gcaccgctac ctgctggcaa tccagaacgc gcctgtacca
    17401 ggaaaatcgg cgctgaaagc cgtgcagtta cggcttgatc agaacagcag cgatccgata
    17461 ttcgccaccc gccagatggc aaaaacgctg cctgctccgc tcaaccgctg ggttggcagg
    17521 ctggctgacc aggcctggca tgtggtgatg gtggaggctg ttcattatat ggaagtggac
    17581 tggcgcgaca gcgtggtgaa accgtttaac gagcaactgg caaataacta tccgtttaat
    17641 ccgcgttctg cacaggatgc ctcactggat gccttcgaac gcttctttaa accggatggc
    17701 atactggata ccttctacca gcagaacctg aagctgttta tcgataatga cctgagtctg
    17761 gaggatggcg ataacaacgt cattattcgc gaagatatta ttgcgcaact ggaaactgcg
    17821 cagaaaatcc gtgacatctt cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg
    17881 gaaacggtat cgctttcagg caataaacgc cgcagtgtac tgaaccttga cggtcagtta
    17941 gtcgattaca gccagggccg taactatacc gcccatctgg tctggcctaa caacatgcgc
    18001 gaaggcaacg aaagtaagct gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc
    18061 agcttcagcg ggccgtgggc gcagttccgc ctgttcgggg ccggacaact gaccggagta
    18121 caggatggca actttaccgt gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg
    18181 cataccgaca cggaagataa cccgttcagc ggtgggttgt tcagccagtt tggtctgtca
    18241 gacacactgt actgaggagg cagggatgaa tagtaacgta ctgacacaaa ctatcgttac
    18301 cggcagtgac ccgcgcgggc tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc
    18361 cagccacccg tcacagcctg agctgaactg gaaactggtg gagtcgctgg cgctggcgat
    18421 ttttaaagcc aacggtgtgg atttacacac cgccacctac tatacgcttg cccggacacg
    18481 gacccaggga ctggcgggat tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca
    18541 cgactgggat aagttctggc cgcagggcgg cccggcgcgt actgaaatgc tggactggtt
    18601 taactcccga accggcaata ttctgcgtca gcaaatctcc tttgcggaat ccgacctacc
    18661 actgatctac cgcacagagc gggcattgca gcttatctgc gataagctcc agcaggtgga
    18721 actgaagcgc gttccgcgcg tggagaatct gctctatttt atgcagaaca cgcgtaaacg
    18781 gcttgaaccc cagctgaaga gtaacactga gaacgccgca cagaccacgg tcagaacgct
    18841 gatttatgtc ccggaaacac aggcatcttc cacaccagaa gcggtagtgc ctcccctgcc
    18901 cggcctacct gagatgaaag tggaagtgcg cagtctgaca gagaatcccc cacaggccag
    18961 tgtgataaag caaggcagta cggtaagagg gtttatcgca gggatcgcct gttcagtggc
    19021 tgtcgcctca gcattgtggt ggtggcaggt ctatccggtg cagcagcaac tgttacaggt
    19081 taacgacacc gctcagggcg cagcaacggt gtggatggcc tcacctgaac tcgaaaacta
    19141 tgagcgcagg ctgcaacaac ttcttgatac ctccccggta cagccgctgg aaaccgggat
    19201 gcagatgatg cgtgttgccg acagtcgctg gccggaaagc ctgcaacagc aacaggcctc
    19261 gacacaatgg aatgaggcac tcaaaacccg cgcacagagt agcccgcagt tgcgtggctg
    19321 gttgcagacc cgccaggact tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga
    19381 gggactaacc ctttcctata tcaaaaatgt catctggcag gcggagcggg gactggggca
    19441 ggaaacaccc gttgagtctc tgttgacgca gtaccatgat gcccgtgcgc agaagcagaa
    19501 tacagatgcg ctggaaaaac aaattaatga gcgactcgaa ggcgtgttaa gccgctggct
    19561 gctgctgaag aataacgtca tgccagaggc ggcaaccggt accacggccg aaaaataatt
    19621 atttaattaa aggagattat catggcaaat ataagttatt tatctttgag tggagagaca
    19681 caagggctaa tttcagcagg ttgctcaacg cttgattctg tcggaaacaa agcgcagcca
    19741 gaacataaag atcagatcat ggtttatgcg ttaatgcatt ccatctcaag aagtcaaaat
    19801 gtcaatcatc atgaattaat tatcaccaaa ccggttgata aatcctcacc attgctggca
    19861 aaagcactta gtgacaatga aaaaatggca atatgtgaat ttatactcta ccgaaccagt
    19921 aaagcgggta tttaccagcc ttattataaa ataaatctgt caaaagcccg aatatcaagt
    19981 attgattttg tcacaccgca tgctgtcctg gaaaaagaac tggaaccaca ggagagaatt
    20041 gcctttattt atgaagatat ttcctgggag catacgttag ccggtacgaa tgcaatgagt
    20101 aaatggcagg atcgtgtaca gtaacttact gtaataacat aataaattac tattgcggag
    20161 agataaaatg gctggtttaa ggaataacaa caacacacag aatgcacagt gggctgatta
    20221 tgtgggtgac attcttcggg gggctcagcc gataaaccag ctagtacctc agcaccctta
    20281 tcttaacgac gtcccactca ttgatgagtt aaggcatcag aacactcatc atgttattct
    20341 cttgacactt gatgttgcaa aaaaaatact cagcccaata acatcatttg attacattca
    20401 ttttatcact acacatcctt caggtataaa agatacactc gcctggttag ttaacgcgag
    20461 taaattaatg actgagtttg atgacaatgg gaaaattata tttaacctta atgccttaaa
    20521 gtatacaaaa gcaagttact ttgaaatact tggagagaaa tatataaaaa tcacaacatc
    20581 cagcccctgg cttttagaaa agctcggtaa atatattttc tcttcacgag cgcctcaagt
    20641 tctggaactc gctattggct ggcgaggagc tttatctgaa tcaataaaag gagttaagtt
    20701 ctgtatctgg ttttcagtag cctggagaac catagaattt attatgagtt cagagcgaga
    20761 tctggttaat tttcttggcg atttttcaat ggatgtggcc aaagcagtta tagccggtgg
    20821 tgttgcaacc gctattggaa gcctgacttc ttttgcctgt gttagctttg gctttccagt
    20881 aattcttgtc ggaggagcaa ttttactgac agggatagtg tgtacagttg ttttaaatga
    20941 aatcgatgct caatgccatt tatcagaaaa attaaaatat gcaattagag atggactaaa
    21001 acggcaacag gaacttgata aatggaaaag ggaaaacatg actccattta tgtatgttct
    21061 taacactcca cccgtgatat gaggattaac catgcgttta catgttaaat taaaagagtt
    21121 tctttcgatg ttttttatgg ccatattatt tttccctgca ttcaatgctt ctttgttttt
    21181 taccggagtt aaacctctat attcaataat aaaatgctcc acggaaatat tttatgattg
    21241 gagaatgttg attctgtgct ttggtttcat gtc
//
LOCUS       DAZFVO020000086        21227 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFVO020000086 DAZFVO020000000
VERSION     DAZFVO020000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 21227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.086"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(251..1255)
                     /gene="argF"
                     /locus_tag="ACHKNB_RS14660"
                     /old_locus_tag="pgaptmp_002932"
     CDS             complement(251..1255)
                     /gene="argF"
                     /locus_tag="ACHKNB_RS14660"
                     /old_locus_tag="pgaptmp_002932"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGU4196406.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1417..1833
                     /gene="rraB"
                     /locus_tag="ACHKNB_RS14665"
                     /old_locus_tag="pgaptmp_002933"
     CDS             1417..1833
                     /gene="rraB"
                     /locus_tag="ACHKNB_RS14665"
                     /old_locus_tag="pgaptmp_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HGU4196407.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1879..2382)
                     /gene="yjgM"
                     /locus_tag="ACHKNB_RS14670"
                     /old_locus_tag="pgaptmp_002934"
     CDS             complement(1879..2382)
                     /gene="yjgM"
                     /locus_tag="ACHKNB_RS14670"
                     /old_locus_tag="pgaptmp_002934"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4196408.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHINYALGCTGHVDCE
                     VRMLREL"
     gene            2575..3771
                     /gene="yjgN"
                     /locus_tag="ACHKNB_RS14675"
                     /old_locus_tag="pgaptmp_002935"
     CDS             2575..3771
                     /gene="yjgN"
                     /locus_tag="ACHKNB_RS14675"
                     /old_locus_tag="pgaptmp_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HGU4196409.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKLWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3827..6682)
                     /gene="valS"
                     /locus_tag="ACHKNB_RS14680"
                     /old_locus_tag="pgaptmp_002936"
     CDS             complement(3827..6682)
                     /gene="valS"
                     /locus_tag="ACHKNB_RS14680"
                     /old_locus_tag="pgaptmp_002936"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGU4196410.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYRETLDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6682..7125)
                     /gene="holC"
                     /locus_tag="ACHKNB_RS14685"
                     /old_locus_tag="pgaptmp_002937"
     CDS             complement(6682..7125)
                     /gene="holC"
                     /locus_tag="ACHKNB_RS14685"
                     /old_locus_tag="pgaptmp_002937"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HGU4196411.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7259..8770)
                     /gene="pepA"
                     /locus_tag="ACHKNB_RS14690"
                     /old_locus_tag="pgaptmp_002938"
     CDS             complement(7259..8770)
                     /gene="pepA"
                     /locus_tag="ACHKNB_RS14690"
                     /old_locus_tag="pgaptmp_002938"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HGU4196412.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8924..8974
                     /gene="ytgA"
                     /locus_tag="ACHKNB_RS14695"
                     /old_locus_tag="pgaptmp_002939"
     CDS             8924..8974
                     /gene="ytgA"
                     /locus_tag="ACHKNB_RS14695"
                     /old_locus_tag="pgaptmp_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HGU4196413.1"
                     /translation="MSIIILATISTTQENK"
     gene            9037..10137
                     /gene="lptF"
                     /locus_tag="ACHKNB_RS14700"
                     /old_locus_tag="pgaptmp_002940"
     CDS             9037..10137
                     /gene="lptF"
                     /locus_tag="ACHKNB_RS14700"
                     /old_locus_tag="pgaptmp_002940"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4196414.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10137..11219
                     /gene="lptG"
                     /locus_tag="ACHKNB_RS14705"
                     /old_locus_tag="pgaptmp_002941"
     CDS             10137..11219
                     /gene="lptG"
                     /locus_tag="ACHKNB_RS14705"
                     /old_locus_tag="pgaptmp_002941"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4196415.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11338..12840)
                     /gene="yjgR"
                     /locus_tag="ACHKNB_RS14710"
                     /old_locus_tag="pgaptmp_002942"
     CDS             complement(11338..12840)
                     /gene="yjgR"
                     /locus_tag="ACHKNB_RS14710"
                     /old_locus_tag="pgaptmp_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HGU4196416.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGNNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12918..13916)
                     /gene="idnR"
                     /locus_tag="ACHKNB_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(12918..13916)
                     /gene="idnR"
                     /locus_tag="ACHKNB_RS14715"
                     /old_locus_tag="pgaptmp_002943"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HGU4196417.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            complement(13983..15302)
                     /gene="idnT"
                     /locus_tag="ACHKNB_RS14720"
                     /old_locus_tag="pgaptmp_002944"
     CDS             complement(13983..15302)
                     /gene="idnT"
                     /locus_tag="ACHKNB_RS14720"
                     /old_locus_tag="pgaptmp_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HGU4196418.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYVNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15367..16131)
                     /gene="idnO"
                     /locus_tag="ACHKNB_RS14725"
                     /old_locus_tag="pgaptmp_002945"
     CDS             complement(15367..16131)
                     /gene="idnO"
                     /locus_tag="ACHKNB_RS14725"
                     /old_locus_tag="pgaptmp_002945"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGU4196419.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(16155..17186)
                     /gene="idnD"
                     /locus_tag="ACHKNB_RS14730"
                     /old_locus_tag="pgaptmp_002946"
     CDS             complement(16155..17186)
                     /gene="idnD"
                     /locus_tag="ACHKNB_RS14730"
                     /old_locus_tag="pgaptmp_002946"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HGU4196420.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17403..17966
                     /gene="idnK"
                     /locus_tag="ACHKNB_RS14735"
                     /old_locus_tag="pgaptmp_002947"
     CDS             17403..17966
                     /gene="idnK"
                     /locus_tag="ACHKNB_RS14735"
                     /old_locus_tag="pgaptmp_002947"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HGU4196421.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(17970..18989)
                     /gene="ahr"
                     /locus_tag="ACHKNB_RS14740"
                     /old_locus_tag="pgaptmp_002948"
     CDS             complement(17970..18989)
                     /gene="ahr"
                     /locus_tag="ACHKNB_RS14740"
                     /old_locus_tag="pgaptmp_002948"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HGU4196422.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIKSAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19185..19269
                     /locus_tag="ACHKNB_RS14745"
                     /old_locus_tag="pgaptmp_002949"
     tRNA            19185..19269
                     /locus_tag="ACHKNB_RS14745"
                     /old_locus_tag="pgaptmp_002949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19219..19221,aa:Leu,seq:caa)
     gene            19433..20701
                     /locus_tag="ACHKNB_RS14750"
                     /old_locus_tag="pgaptmp_002950"
     CDS             19433..20701
                     /locus_tag="ACHKNB_RS14750"
                     /old_locus_tag="pgaptmp_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4196423.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKVVQKEARAENSGAYTFEA
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MMQWWADYLDMNRNKYITPYDYSKYKEILK"
     gene            20808..>21227
                     /locus_tag="ACHKNB_RS14755"
                     /old_locus_tag="pgaptmp_002951"
     CDS             20808..>21227
                     /locus_tag="ACHKNB_RS14755"
                     /old_locus_tag="pgaptmp_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HGU4196424.1"
                     /translation="MAELLREQEVKIALIEWLYKKGMLSDATIINEMVVANWSRRADL
                     AIANGSLQAFEIKSDFDSLKRLDGQLKIFKSSFEKVTIVCAPKFTSEVSKRVSSDVGV
                     VEFQSNNSSVRFKIIQKGRVCSRLNKFVYLGFLLKSEL"
BASE COUNT         5231 a         5293 c         5217 g         5486 t
ORIGIN      
        1 ttaggatatg tttatggtaa tgaagaaaaa acaaatgaaa ttgtaaaaag caatccgggt
       61 tatgtcgtta agatttttga agaaaagatg gttaatgaat taaaaattag aacaagaatg
      121 atgaaaatcc tccggaagat ttacgtatag caatagaaaa atatggatgg aataattgaa
      181 ctattactgc ataaaataat gtgatgccgg gatgtttttg tatttcccgg catctttata
      241 gcgatatcaa ttatttactg agcgtcgcga ccatcaccgc tttgatggta tgcatgcggt
      301 tttccgcctg atcaaaaaca atgctggcgg cagattcgaa gacctcatca gtcacttcca
      361 ttccgccatg taggccaaac tcttccgcca ttttcttacc aagcgtcgtc tggtcgtcat
      421 gaaacgcggg caggcagtgg aggaatttga cctccgggtt accggtcaac tgcatcatct
      481 tgctgttcac ctgataatca cgcagtaatg caatccgttc tgcccatttc tcttttgctt
      541 cccccatcga cacccataca tcggtataga taaagtcagc accttcaact cctttggcta
      601 catcttccgt cagcgtaata ttcccgccat tttgctgtgc cagggcgcgg cattccgtaa
      661 ccagcgcagc ttccggccag catgcctgtg gcgcgaccag acgcaaatcc agaccggtaa
      721 gcgccgcagc ttcgagcatc gaattgccca tgttgttacg tgcgtcacct gcatagacca
      781 gcgtcatttc gttgaatgct ttaccgggta aatgctcctg catggtgagg agatccgcca
      841 gcaactgcgt ggggtgaaac tcattggtca ggccgttcca taccggcacg ccagcgtatt
      901 ccgccagtgt ttcgacaatc tcctgaccat agccgcgata ctgaataccg tcatacatgc
      961 gacccagcac gcgggcagtg tctttaatcg actctttatg accaatctga ctgccgcttg
     1021 ggccgagata agtaacgcga gcaccctggt catatgcggc aacttcgaaa gagcatcggg
     1081 tacgggtcga gtctttttcg aagatgagcg cgatgttttt accagtgagt ttggcttctt
     1141 ctttgccgct tttcttatcg gctttcagct tcgcggctaa ctgcagcagg ctgttgagtt
     1201 cagctggcgt gaaatcgagt aatttcagga aatgcttatg ataaaacccg gacatagatc
     1261 cctcctgtgg ctaacgcctt aatgaattaa aattcaattt atatggatga ttattcattt
     1321 gcaagtctaa agcataaatc tttgtcacaa aggtggaggc aatgtcagtg gtgtgtgaca
     1381 ataagagtat cggcaggaca ttaagaggaa tgagccatgg caaacccgga acaactggaa
     1441 gaacagcgtg aagaaacacg tttgattatt gaagaattac tggaagatgg cagcgatccg
     1501 gacgcgctgt acaccatcga acaccatctt tccgcagacg atctggaaac cctggaaaaa
     1561 gcagcagttg aagcgtttaa actcggttac gaagtgaccg atccagaaga gctggaagtt
     1621 gaagatggtg atatcgtgat ttgctgcgac atcctcagcg agtgcgcgtt gaatgccgat
     1681 ctgatcgatg cccaggttga acaactgatg acgctggcgg agaaatttga cgttgagtac
     1741 gacggttggg gcacttactt tgaagatccc aatggcgaag acggcgacga tgaagatttt
     1801 gtcgatgaag acgatgacgg tgttcgccac taattaattg attaaaatgc ccgatagcac
     1861 acactagcgg gcattttttt agagttcgcg tagcatccgc acttcacaat cgacatggcc
     1921 cgtgcagcca agcgcatagt tgatatgctc aaagcccaaa tgctcataaa gcgcaatggc
     1981 ttcctttaaa aaagcggtcg tttccagata gcagcgtttg aagcccatct ctcgtgcctg
     2041 ctccatcgcc attaaggcca gttttttcgc cagcccttta ccgcgaatag ccggaagaaa
     2101 atacatcttt tgcagttcgc aaatgtccga ttcactcccg gttaatggcg caatcccacc
     2161 gccgccgacc acttcaccat cgaactcaac cacccaatat gcatggccag gctgactata
     2221 cacttgatac aactcgtcaa ggttcggatc ggcgacggtg tagcctttat cagcggtaag
     2281 accgtattcg gcggatacct gacgaatgac gcgggcgata gcgggattat cctgctgcgt
     2341 caggcgacgc attacaggtg attgcggcgc aatgttattc atagtttaac ccaataaaaa
     2401 taaagtaatt atacatttgt ttaataccac tctcttctta cgtgcgcaag cgacttataa
     2461 agtaataaag tttttgccag cctattactg ataagagtat tgtaacgtgg cagaacattt
     2521 tgctgtctgt atatataaat tatatcaatg gattttttaa ttcagggaat ttttatggct
     2581 caagttatta atgaaatgga tgttccgtcc cattcgtttg tttttcatgg tacaggtgag
     2641 agatattttc ttatttgtgt ggtgaatgtg ttgttaacga ttataacgct aggtatctat
     2701 ttgccatggg cattaatgaa atgtaagcgt tatctctatg ctaatatgga agttaacgga
     2761 caacgatttt cttatggaat tactggtggg aatgtttttg ttagttgtct tgtttttgtt
     2821 ttttgctatt tcgcaatctt aatgacagtg tcagcagata tgccacttgt tggctgtgtt
     2881 ttgactttgt tactgttggt tttgcttata tttatggcag caaaaggact gcgttatcag
     2941 gccttgatga ccagtctcaa cggcgtaaga tttagtttta attgctctat gaaagggttc
     3001 tggtgggtaa cctttttctt gccgatttta atggccattg ggatggggac tgttttcttt
     3061 atctcgacaa agatgctaca tgccaatagt tcaagtagtg ttattatatc tgtggttctg
     3121 atggcaatag ttggtattgt ttccattggt atttttaatg gtactttata tagcctggta
     3181 atgagttttc tctggagcaa taccagtttc ggtatacatc gtttcaaggt gaaattagat
     3241 actacgtatt gtataaaata tgccattctc gcatttttag ctttattacc ttttctcgct
     3301 gttgctggtt atattatctt cgatcaaata ttaaatgcat atgatagttc tgtgtatgca
     3361 aatgatgata ttgagaattt acagcaattt atggaaatgc aacgtaaaat gataatcgcg
     3421 cagttaatct attattttgg gattgctgtt agcaccagtt atttaacggt gtcgttgcga
     3481 aatcatttta tgagcaacct gtcactgaat gatgggcgta ttcgttttcg ctcaacttta
     3541 acgtaccacg gtatgcttta tcgcatgtgt gcgttggtgg tgatatccgg gattacgggc
     3601 ggtctggctt atccactgct gaaattatgg atgattgact ggcaggcaaa aaatacgtat
     3661 ttgctgggcg atttggatga ccttccttta atcaataaag aagaacaacc agataaaggc
     3721 ttcttagcca ggatttcacg gggaattatg ccttctttac catttctgta agagataaaa
     3781 aaggccggag catgctccgg ccttcgtttt cattactgtg ttttgattac agcgcggcga
     3841 taacagcctg ctgttcaatc agcttcgctt tcgcttctgc atagccttcc agcttttcac
     3901 gctctttcgc gatgaccgct tccggtgcgc gggcgacaaa gccttcattc gccagcttgt
     3961 tctcgatacg gctgatttca ccttcaatct tcgccacttc tttcgccaga cgcgccagct
     4021 catcttcttt gttgatgagg ccagccatcg ggatcagcag ctctgcaccg tcgatgatct
     4081 tagtaacgga aaccggacct ttgtcatcgg caggcagcac ggtgatgctt tccagacgcg
     4141 ccagcgtttg caggaagcca cggttttcat ttacgcgacg ttctgcatcc gcgctgcaac
     4201 cacgcagtag cagctccagc ggtttgcccg gcgcgatgtt catttctgca cggatgttac
     4261 gtaccgcaac gatcgcctgc ttcagccact ctgtgtcggc cagtgcggct tcatcaacct
     4321 gagatgcatc gtactgcggg aacggctgca gcatgatggt gtcggcagtg ataccgcaaa
     4381 gtactttcac acgctgccag atggtttcgg tgatgaacgg aatgatcgga tgcgcgaggc
     4441 gcagcagacc ttccagcacg gtcaccagcg tatggcgagt accgcgcagt tcggcttcgg
     4501 tgccaccgtt cattaccggc ttagtcagct ccagatacca gtcacagaac tggttccagg
     4561 tgaactcata cagaatgcct gcggcgatat cgaagcggaa gctgtccagc gtttcgcggt
     4621 acgctttgat ggtctggttg aactccgcca gaatccagcg atccgccagc gacagcgtca
     4681 tctcgccgcc gttgaagccg caatcctgac cttctgtgtt catcagcaca aagcggctgg
     4741 cgttccacag cttgttacag aagttacggt aaccttccag gcgcttcata tcccagttga
     4801 tgtcacgacc ggtagacgcc agtgccgcca gggtgaagcg cagggcatcg gtgccgtgcg
     4861 gctcaatgcc gttcgggaac tgtttctcgg tgcgcttacg gattttgtcc gccagctgcg
     4921 gctgcatcat attgccggta cgtttttcca gcagttctgg cagcgaaata ccgtcaacca
     4981 tatccagcgg gtcgataacg ttacccttgg atttggacat cttctggcct tcgtcgtcac
     5041 gaatcagacc ggtcatgtaa acagtgtgga acggcacctg cggtttgcca ttttcatctt
     5101 tgatgaagtg catggtcatc atgatcatgc gggcaatcca gaagaagatg atgtcgaaac
     5161 cagataccat cacgctggtt gggtggaact gacgcagggc gtcggtgttt tccggccagc
     5221 caagggtaga gaaggtccac agcgcagaag agaaccaggt atcgagaacg tcttcgtcct
     5281 gacgcaggac aacatcagca ccgaggttat tttctttacg cacttcgtct tcgttgcggc
     5341 caacataaac gttacccgct tcgtcatacc atgccgggat acggtgaccc caccacaact
     5401 gacgagagat acaccagtcc tgaatatcgc gcatccagga gaagtacatg ttttcgtact
     5461 gcttcggtac gaactgaatg tcgccgttct caaccgcttc aactgccggt ttcgccagga
     5521 catcggcacg cacgtaccac tggtcggtca gcattggttc gatgaccacg ccgccacggt
     5581 cgccgtaagg aacggtcagg tcatgtggtt taatttcttc cagcaggccg agtgcgtcaa
     5641 ccgcagcaac gaccgcttta cgtgcagcaa aacgctccag tttctggaat tctgccggga
     5701 tctcgctgga ataaacgtca gattcgttac ctttggtatc gaacacctgg gcactttcac
     5761 ggatatcgcc gtcaaaggtc aggatgttga tcatcggcag ggcgtgacgt ttaccgactt
     5821 catagtcgtt aaagtcgtgc gccggggtga tcttcacgca gccggtgcct ttttccatgt
     5881 cggcgtgttc gtcgccaacg atcggaatac gacggttaac cagcggcaga atgacatatt
     5941 tgccaatcag atctttgtaa cgcggatctt ccgggttaac ggctacgcca gtatcgccca
     6001 gcagggtttc cggacgggta gtcgcaacca ccagataatc tttaccgtct gcggttttcg
     6061 cgccgtcagc cagcggatag cggatgtgcc acatcgaacc tttcgattcg cggttttcca
     6121 cttccaggtc agagatagcg gtgcgcagtt tcgggtccca gtttaccagg cgtttgccac
     6181 ggtaaatcag gtcttcttta tacagacgaa cgaaaacttc tttcaccgca ttggacaggc
     6241 cttcgtccat ggtgaagcgt tcacgctccc agtccacgga gttgccgaga cggcgcatct
     6301 gacgggtaat ggtgccgcca gattccgctt tccattccca gattttgtcg atgaaagctt
     6361 cgcggccgta gtcgtgacgg gttttacctt cttctgcggc aatcttgcgc tcaacgacca
     6421 tctgggtagc gatcccggcg tggtcagtac cgacctgcca cagggtgttt ttgccctgca
     6481 tgcgctgata gcggatcatg gtatccatga tggtttgctg gaaggcgtga cccatatgca
     6541 aactgccggt gacgttcggc ggcgggatca tgatgcagaa actttcctgg ctttcatcgc
     6601 cattaggctt aaagtagccc tgcttttccc agtgctcgta aagcggctgt tcgatatctt
     6661 gtgggttata tgtcttttcc attatttcca ggttgccgta ttcaggttga aaccagccac
     6721 gcggtaggct ttatagcgtt cgcgcgccag ttgtttcaga gaatcttcat aaggaacgaa
     6781 gtctaccact tctgtgaaag cggtggcaaa atctgcaaag cttgttcgca gactaatcaa
     6841 tatatcgcgc gggctgctgc tgcgtttttg cggccaggcg atctccaccg gcgcaccgcc
     6901 gcgcggtcct tctcccgcta aattatgcgg aacaaagctt tctgccggac gcgcccacag
     6961 ggcttcatcc agccggtaag cctgcttttc atcttcacag gcgatgagca cgcgcttacc
     7021 actgcgccaa cgttctgcgg caatttcaca caccagctgc tcaacggcgc ttaagccatc
     7081 gacggtggtg tcattgtcca gaaggtagaa cgtcgcgttt ttcataaatg gggcttcttg
     7141 tggtggattt aaatattgct gcattcctca ccctaaaaga tgagcaatga atttctattg
     7201 cgctcgatcg gttccctctc ccttagggag agggttaggg tgagggtaaa acgcaaactt
     7261 actcttcgcc gttaaacccg gcgcggttta acaggaactg tgccagcaac gctaccggac
     7321 gaccggttgc gcctttcgct ttaccagaac gccaggcggt accggcgata tccaggtgcg
     7381 cccagttgta cttacgggta aagcgtgaca ggaagcaacc tgcggtaatc gccccaccag
     7441 gacgaccgcc aatgttcgcc atatcggcaa aattggactc cagttgttcc tgatactcgt
     7501 cacccagcgg taagcgccat gcgcggtcac cggattgttc agacgcggca atcagttcat
     7561 gggccagcgg attgtggttc gccatcagac cagtaatgtg atgacccagc gcgatcacgc
     7621 aggcaccggt cagcgtcgcc acgtcaatca ccgcttccgg ctcaaaacgc tcaacgtaag
     7681 ttaacacgtc gcacagtacc aggcggcctt cagcgtcggt gttgagcact tcaacggttt
     7741 gaccagacat ggtggttaac acatcgcccg gacgataggc gcgaccgcct ggcatgtttt
     7801 cacaacctgc caacacgccg ataacgttaa tcggcagttg tagctccgcg accatccgca
     7861 tcacgccgta aaccgctgcc gcaccgcaca tatcgtactt catctcatcc atgccttctg
     7921 aaggcttgat cgagataccg ccggagtcga aggttaaacc tttacccacc agcacgattg
     7981 ggcgtgcatc ttccgacgcg ttgcctttgt actcaatcac cgacatcagc gattcgtttt
     8041 gcgaaccctg accgaccgcc agataggaat gcatccccag ctctttcatc tgctgttcgc
     8101 cgataacgcg ggtgataaca ttcttgctgt agctgtcagc cagctggcgc gcttgtgaag
     8161 cgaggtaagc ggcgttacag atattcggcg gcatattgcc aagatccttt gctgctttaa
     8221 tcccggcggc aatcgccaga ccgtgctgga tcgcgcgctc accgctggtc agttcacggc
     8281 gggtcggcac gttgaacacc attttacgca gcggacgacg cggttcgctc ttgttcgttt
     8341 tcagctgatc gaaactgtag agcgtctctt ttgccgtttc gacagcctga cgcactttcc
     8401 agtagttgtt acggccttta acgtgcagtt cagtcagaaa gcagaccgct tccattgagc
     8461 cagtatcatt cagcgtatta atggttttct gaataacctg cttgtactga cgctcatcca
     8521 gctcacgttc tttgccgcaa ccaataagga gaattcgctc ggaaagtaca ttcggaacat
     8581 ggtgcagcaa caatgtctgc cccggttttc cttccagttc gccccgacgt agcagggcgc
     8641 tgatgtaccc atcgctgatt ttatcgagct gttctgcaat cggagaaagg cgacgtggtt
     8701 cgaagacgcc cacgacgatg caggcactcc gctgtttctc cgggctaccg ctttttacac
     8761 taaactccat gcactacgct cctgaatctt aaagacaacg gcggtggcta cagatagaat
     8821 tgcaagcttt cgtaactcat gtccgctgtt gcgatgactt cgtgttaatc ttaacgttat
     8881 tacggcattg gcacgtcaga acaaaatctg agaggtgaat ccgttgagta taattatctt
     8941 agcgacgatt tcgacgactc aagagaataa atgacgttta agccatgaaa caagctaaaa
     9001 tcctgcaaaa gacgagtttt tacgggcgta tttaaagtga taatcataag atatctggtg
     9061 cgggagacgc tcaaaagcca gctggcgata ctcttcatct tgcttttgat cttcttctgt
     9121 caaaagttag tgaggatcct cggcgcagcg gttgacggcg atattccggc gaatctggtg
     9181 ctctcccttc tcgggttggg cgtgccggaa atggcgcagc ttatcctgcc attaagcctg
     9241 ttcctcgggc tgctgatgac gctgggcaaa ctgtataccg aaagtgaaat tacggtaatg
     9301 catgcctgcg gcctgagcaa agcggttctg gtgaaagcgg caatgatcct tgcggtattc
     9361 acggcaatcg ttgcggcggt taacgtgatg tgggcgggac cgtggtcatc gcgtcatcag
     9421 gatgaagtgt tagcagaagc gaaagcgaac cctggcatgg cggcgctggc gcaagggcaa
     9481 ttccagcaag cgactaatgg cagttcggtg ctgttcatcg aaagcgttga cggcagcgat
     9541 ttcaaagatg tgttcctcgc gcaaattcga ccaaaaggta atgcacgtcc ttctgtggtg
     9601 gtggccgatt ccggacattt aacccagctg cgcgacggct cccaggtcgt cactctcaac
     9661 cagggaacgc gcttcgaagg cactgcactg ttacgtgatt tccgcattac ggacttccag
     9721 gattatcagg cgatcattgg tcaccaggcg gtggcgctcg acccgaacga taccgaccag
     9781 atggacatgc gtacattgtg gaacactgac accgatcgtg cccgcgcaga actgaactgg
     9841 cgtatcacgt tggtattcac cgtgtttatg atggcactta tggtcgtacc gttgagcgtg
     9901 gttaacccac gtcagggacg cgtactgtcg atgctgccag ccatgctgct gtatctactt
     9961 ttcttcctga tccagacctc cctgaaatcg aacggcggta aaggtaagct ggacccgacg
    10021 ctgtggatgt ggaccgttaa cctgatttat ctggctttgg cgattgttct caacctttgg
    10081 gacactgtgc cggtccgccg cctgcgcgcc agtttttcgc gtaaaggagc ggtgtgatgc
    10141 aaccttttgg cgtacttgac cgctatatcg gtaaaactat tttcaccacc atcatgatga
    10201 cgctgttcat gctggtgtcg ctgtccggca ttatcaagtt tgtcgatcag ctgaaaaaag
    10261 ccgggcaggg gagttacgac gcgttaggcg caggaatgta taccttgctg agcgtgccga
    10321 aagatgtgca aatcttcttc ccgatggcgg ctctgcttgg ggcgttgctt ggtcttggga
    10381 tgctggcgca gcgcagcgaa ctggtggtga tgcaggcttc tggttttacc cgtatgcagg
    10441 tggcgctgtc ggtgatgaaa accgccattc cgctggtctt gctgacgatg gcgattggtg
    10501 aatgggtcgc gccgcagggc gagcagatgg cgcgtaacta ccgtgcgcag gcgatgtacg
    10561 gcggctcgtt gctctctacc cagcaaggct tatgggcgaa agatggcaac aacttcgtct
    10621 acattgagcg ggttaaaggt gacgaagagt taggtggcat cagcatttat gcctttaacg
    10681 agaatcgtcg tctacaatcc gtacgctatg ccgctactgc gaagtttgac ccggaacata
    10741 aagtctggcg tctgtcgcag gttgatgaat ctgatttgac caatccgaaa cagattaccg
    10801 gttcgcagac ggtgagcggc acctggaaaa ccaacctcac gccagacaaa ctgggcgtgg
    10861 tggcgctgga cccggatgca ctctcaatca gcggtttgca caactacgtg aagtatctga
    10921 agtcgagcgg tcaggatgcc ggacgttatc agctcaatat gtggagcaaa atcttccagc
    10981 cgctatccgt ggcggtgatg atgctgatgg cgctgtcgtt catctttggc ccactgcgta
    11041 gcgtaccgat gggcgtgcgt gtggtcaccg gtatcagctt cggttttgtc ttctacgtac
    11101 tggaccagat ctttggcccg ctgacgttgg tttatggcat cccgccgatc atcggcgcac
    11161 tgttgccaag cgccagcttc ttcttaatca gcctgtggct gttaatgaga aaatcgtaac
    11221 caagcaaggg agcgaaagct ccctttcaca ttgttgataa agtcaaatac gagttcgatc
    11281 ggtcccctcg cccctttggg gagagggtta gggtgagggg cacagaccgc acctgactta
    11341 ccttcttctt ccccccagca aactccccaa catcccgcgt acaatctgat tcgtcacttg
    11401 gcgggcggcg cttttcgcca ttgtttgcac cacaccatct ttcttcccgc cgcgtggtcc
    11461 ggtagtgcca aacaaaatat cctttaatcc accaagaatg ccgtcatcca ccgccacctc
    11521 tttccctttc gcggggggat tattttgctg ctcggcgctt gcctgtacgc ctttttgcaa
    11581 catctcatag gcggattccc ggtccacctc atcctcgtac ttgccataca ccggagagtg
    11641 attaatcaag ccattacgct catcttccgt caccggtccc atccgcgaac aaggcgcaat
    11701 caccatcgcc cgttccacca ctgaaggact tcctttcaca tcaagaaacg agattaacgc
    11761 ctcgccggtc cccagttcct ggattgcctt ttcggtatca aatgccggat tggcccgcat
    11821 ggtttgcgcc gcagctttta ccgctttctg atctttgggc gtaaaagcac gcaaagcgtg
    11881 ttgaacgcga ttaccgagct gcccgagcac attatccgga atatcagacg ggttttgcga
    11941 aacgaaccag acgcctacgc cttttgagcg aataagccgt atcacctgct caatcttatc
    12001 cagcagtacc tgcggtgcat cgttaaacag cagatgtgcc tcgtcgaaga aaaacaccag
    12061 ttttggtttc tccagatcgc ctgcttccgg caattgttca tacaactctg aaagcatcca
    12121 cagcaggctg gcggcgtaca gtttcggcat ctgataaagc ttctcggcgc tgaggatatt
    12181 gataacgcct ttaccgttgg tatcggtgcg catccagtct ttgatatcca gcatcggctc
    12241 gccaaagaag tgtgctgcgc cttgctgttc cagcgataac agcccgcgct ggatggcacc
    12301 aaccgatgcg ctactaatat taccgtactg attctggaaa gatttggcgt tattgccgat
    12361 gtactgggta atcgcccgca aatctttaaa gtcgagcagt aacagcccct gatcgtcagc
    12421 aatgcggaag atgatattca gcacgccaga ttgcacatcg ttgagattca acagccgcgc
    12481 cagcaacagc ggccccaggt ctgaaaccgt cgcccgcacc ggatggcctt tctcgccaaa
    12541 gatatcccac accaccaccg gattggcatg cggttgccag tcattgacgc cgatattttt
    12601 aagccttgcg agcagttttt ccgacgccgt tcctgcctgc gcgataccgg tcagatcgcc
    12661 tttcacatca gccataaaca ccggcacgcc gatttccgac aatgactccg ccagtttttg
    12721 cagcgtaacg gttttacccg tccccgttgc gccagtaatc agcccgtgac ggttagccat
    12781 tcccggcagt aaaaacagtt ctgtgtccgg cgtgcgggca attaacaggg gttcgctcat
    12841 gagatttcct ccagtttcct gcctggagta taggcaacgt ccggcaaaga gaatacgtgt
    12901 aatgaagaga aaataaccta aagcgtgttg ccttgataaa tctgataccc gaggtcaaca
    12961 gtgttgtggt tgtgatcgtt atttttaatt ttgctcagca acatttgggc ggccatccga
    13021 ccaatatcaa aacggggagt aattacgctg gcgaggcttg ggatcatttg cctgccaatc
    13081 tccagcccat gaaaacccgc aatggaaatt tgttctggca cggcgaggtt tcgctcgcgg
    13141 cacagcagaa gcgcacccat cgcaatatcg tcattggtgc aaaatacacc gtctaaatcc
    13201 gggtttgcgc taagggcatc acgcattaac tgcattccta aatgaatgga tgagatggcg
    13261 cgtggattca tgcgtaatgg ggaaagatta tgcagcatca tcgcatcgca atacccctga
    13321 taacgctgtt catcgcgggt gtcatctttg gaaccgagat acagaatttt gtgcctgact
    13381 cgcttctcca gcatggtaca caccatgtca aaagcggcct gccgattatc aaaaccgacc
    13441 tccatatcca gccgttctcc ctgtacatcc atcaattcca cgaccggaat ggtggctgaa
    13501 cgcagaaatt tcaccgtcct gatggtgtga tatttttccg aaagtataat cccgtcaatg
    13561 ttataagaga gcaggttaat caccgactct tcttcggaat cgcgatcgta attgtaatta
    13621 gcaataaggg tctgataatt atgctcagag gtgacggatt ctattcccgc gagaatgtca
    13681 gcgaagagtt gattttgaaa tgacggaatc aagataccaa gggtataact ttgtgcattc
    13741 aacaacatgc cgggagcgcg attagggata taattgatct cttccataat tttggcgatg
    13801 cgctcgcctg tttcctttgc cacctttttc ggcgagcgga tataacgact cacggtcatt
    13861 tttgttacgc cagccagcgt agcgatatcc tgtaaagaaa ttctgtgatt gcgcataact
    13921 tctcccaacg tcgggggaat cttcaatctt cccccgaaac tacatcacaa tattttattc
    13981 tttcagtgca atacggcgtt aatggctaac acgcccagca aacccataat agaaataagg
    14041 gtttccatca ccgtccaggt acgcaacgtt tcaccaaccg tcagattaac atacccttta
    14101 aatagccaga agccagggtc gtttacgtgt gacgcgatca cgctgcccgc accggttgcc
    14161 agtaccatta aagcgggatc ggcatgggta acgttgataa tcggcaacac cacacccgcg
    14221 gtggtaatgg ccgcgacggt ggcagagccc agagcgatac gcaacagcgc cgcaacagtc
    14281 cagcacatca ataacggcga aagcgtagtt ccggtcatta agtgtgaaat atagtgcccg
    14341 acaccgctat ctactaatac ctgcttaaac gcgccgccgc cagcgataat aaacacaatc
    14401 atcgcgatag cgcctataga atccccaatg atatccatga tttgctcgat ggtgcgtcca
    14461 ttacgtcggc ccagtgtgaa aatcgcaata acaatggcaa taaacagcgc aacggcaggg
    14521 ttaccgacaa attcaaagaa gaggcgcacg gtgttagttt tcggtaacgt aatttcacaa
    14581 acggcggcga tagccatcag gatgaccggg atcacggcag cgaaaatact gttccagaag
    14641 gagggcatct cctcttcgct aaacagatga ggattaaata agccttccgg tggtgctttc
    14701 tcaaagcgag ttagcagttt agaaaacagc ggtcctgcga caataactgt cggaatggta
    14761 atgataaatc catacagtaa agtcgttccg agattagcct caaagatagt cgcgatggca
    14821 gtaggccctg gatgtggcgg cagaaaacag tgggttacag agagcgctgc taccattggt
    14881 acgccaacat acaacagggg taatcctgat gatgctacga tggtaaatac caacggcaac
    14941 agcaggacaa aacccacttc aaaaaacatg gcgaggccca caaccagacc ggtgatcact
    15001 agcgcccatt gcacgcgttt tttaccaaaa gtagcaatca gcgtagtggc gatacgttgt
    15061 gccgcacccg tatcagaaat cagcttgcct aacatggccc cgaaaccgag aatcattgcc
    15121 agcccgccga gcgtgctgcc gataccattt tgtatagagt gcaggacggc ctgtgcatcc
    15181 atcccttcgg caaatccgac gacggcagct accagaacga gggcaataaa gccgttaact
    15241 ttaaagccga tcatcaggat aagaagcagc gcgacgcctg ccgcaataat gattaatggc
    15301 atagtcttta tctcttatta gtgccagaaa agcagcgctt tcgcgctgca cgtagggtag
    15361 taggtgttaa acagccacta acatgccgcc atcaacaaac aacaggtggc cgtttacgaa
    15421 atcagaggct tttgaagaaa ggaacaccgc agcaccaatc agttcctgcg gatctcccca
    15481 gcgtgcggcg ggggtccgtt tgcacaacca ggcggtgaag gcttcgtcct caaccagtgc
    15541 tttagtcatt tctgttttga aatagcccgg cgcaataccg ttgacctgaa tattgtggcg
    15601 cgccagctcg acgcacatgc cgcgggtgag catttttacc gcccctttcg atgcggcata
    15661 aggggtgatg gtgtcacgtc ccagttcgct ttgcatcgag caaatattaa taactttacc
    15721 tgccttgcgt tcaaccatgt gacgagttac cgcttgcgat accaggaaca cggcggtctg
    15781 gtttactgcg atcacatcat tccactcttg ttcagggaac tcagtaaaag gatgacggcg
    15841 ctggataccg gcgttattca ccagcacatc aatggggccg atgtcctttt cgatatgttc
    15901 aacggcggca tcaatttcat gtttatgagt aacattaaaa ggtgcggcaa cggcctgaat
    15961 accctcctgg tggagttttt ctacagcaag ttctgcgcgt tcggcagtaa tatcattaat
    16021 aattatttgt gcgccatatt tacccaggcc ggttgccagt aaaaagccaa tgccctgtgc
    16081 tgaaccggta atcaagatat tttttcctgc cagtgaaaat agatcgttca ttgtattatt
    16141 cctttattta tctcttagaa aacaagctgg acttttgctg cctgggtttt atcaccggcg
    16201 aaacgtagcg cctcttccag gtcagtgaag ggatattcag cactcagtaa aggcagtgga
    16261 ttgataacgc cattcgccag ccatgacact gcggtattaa attcgctggt aaaacggaaa
    16321 gagcctctga gtgaaatctc cttaccaatc aacgtcatca ttgggaattc tgccatcgcg
    16381 cctcccatac ctacctgcac cattacgccg cgtgcacgag tgacctccag acaggtattc
    16441 actgatgaag gatgaccgga cacttcaaag ctgacatcga aatagccttt ttccgctttc
    16501 cagtgatcca tgtcgtcgtt ttgtgggttt acgagcacat ccgcccccat ctctttgccc
    16561 agcgaaaggg aacggggact cacatcagca cagacaattt ccgcggcccc cagtgttttc
    16621 actgcactga caatcaggca gccaatgggt ccaacaccgg aaataaatac tcgcttgccc
    16681 tgtaactcgc cggcctgatg tgcggcatga atcgcgacgg ctaaaggttc ggcaaaagcc
    16741 ataacctttt cgtcagcttt ggccggataa gggacacatt gcgacgtttc gaccatttta
    16801 taacgggtaa aaccaccatc aacatgaggg aaatacatgg cactgccaaa aaaacgcatc
    16861 tctgtacact gattctcgtt atgttcaatg cagtatttgc agtgaccgca cggtttagac
    16921 ggattaatgg ctaccgtttg cccttcatgt aattctgatg agtcgctatg aataacttta
    16981 ccgataactt catgacctaa caccatcggt gcctttatca tgaaattacc tacttttcct
    17041 tcctgataat aatgtaaatc ggaaccgcaa attccacctc gggttatttg tactaatgtt
    17101 ccattattat tccaatctat cgtctgctcg gtaacggcaa cagttttctt gcccgcaaca
    17161 acgcaggact gtgttttcac ttgcataaat tcctcactgg tcaggtagac acctcggaag
    17221 catttaagcg gttttaattg tcattatttg tgatgaagat cacgtcagaa aattgttaca
    17281 ttactatgtt acgcataacg tgatgtgcct tgtaattctt atcagcagaa ataaaaaaac
    17341 gtgaaattat tatgccacca ggcgtagtat cgcagcaggt aagatgattc aggagatttt
    17401 aaatggcggg tgaaagcttt attttgatgg gcgtttcagg gagtggtaaa acattaattg
    17461 gtagcaaggt tgccgcgtta ttatctgcta aatttattga tggtgacgat cttcatccag
    17521 ccaaaaatat agataaaatg tcgcagggta ttccattatc tgatgaagat cgacttccct
    17581 ggctggaacg cttaaatgat gcttcataca gtctttataa aaagaatgaa acaggattta
    17641 ttgtctgttc atcattaaaa aaacattatc gtgatatttt acgcaagggt agcccccatg
    17701 ttcatttcct ctggttagat ggcgactatg aaactatcct cgcgcgaatg cagcgtcggg
    17761 ctgggcattt tatgccggta gcgttactaa aaagtcagtt tgaggcgctg gagcgtccac
    17821 aagcagatga acaggatatt gtgcgcattg atatcaacca tgatattgcc aatgtcaccg
    17881 aacaatgtcg gcaggctgtg ctggcgatac gacaaaacag aatatgtgcg aaagagggca
    17941 gcgcctcaga tcagcgctgc gaatgatttt caaaaatcgg ctttcaacac cacgcggtaa
    18001 cgcgccttac cgtcgcgcac atgctggatg gcgtcgttaa ttttcgacat cgggaacagt
    18061 tcggttgtcg gcgcaacctt gctgcgggcg gcaaagcgca tcagcttacg cagttcataa
    18121 ggcgtgccgg tagcagagcc agagacgctg cgatcgcccg caattaacgt aaaggccgga
    18181 acagacagcg gcgtgagaac cgcaccgaca gtgtggaaat taccgccgta cgtcagcgcc
    18241 tcaaaataag gctgccagtc gaggctgacg ttcacggtat tgataatgag atcaaactgc
    18301 cccgccagtg ctttcagtgc ctgcggatcg cggctattca ccactttatc ggcacccatc
    18361 gccagcaatt cctgctcttt cgccggatta gaactaaagg ccgtcacctc acatcccatt
    18421 gcgtgcagaa gttttatagc gatatgcccc agcccgccaa taccaattac cccaacgcgg
    18481 ctggtagcag tgatatggtg catcaacagt ggtttaaaga ccgtgatacc gccgcacaac
    18541 agcggcccgg cagatttaat gtcgatattt tccggcagtg gaataaccca ttgccagtct
    18601 acacgcaact tctcggcaaa acctccgcga ttcataattg ttggcaccgc accttgctca
    18661 cagttgatct gatttccgct aatacaggcg tcgcagtgac cacagctacg cgctgtccag
    18721 ccaatcccga cacgctgacc gacctgcaaa cctttatcct gcgcggcact cccgagcgcc
    18781 accacgcgac caatcacctc atgcccggca accagcggat attgtgaaaa tccccattcg
    18841 ttatcgatca tcgacagatc ggaatggcag atcccgcagt aatccacctg cacttcaaca
    18901 tcttgtggct tcagctcacc gggatcgtac tcataaactt ccagttcgcc gcccgcttct
    18961 tttgcggcat agctttttat cattgacatt ttttggtcct tctctggtgt ggtctgggaa
    19021 gtgtagagca tggcagggcg gggtgcatgg agtgtgacaa aggttacaca tcgctgtacg
    19081 caatgctgaa aatttcagca cttagcgaga tgcgagcaag ctggcgcttg catggtggcg
    19141 tgcgacaggt ataatccaca acgttttccg catacctctt cagtgccgaa gtggcgaaat
    19201 cggtagacgc agttgattca aaatcaaccg tagaaatacg tgccggttcg agtccggcct
    19261 tcggcaccat tcttacttcc aagaccatcc gagaaagtcc aaatatccct ttaaaatcaa
    19321 tgcttgcagc gattttttcg tcctgcgtct tccgaggttg tccgttgaaa tccggatatc
    19381 attgggggca taattggggg catcttaact tcgattagaa atgtgccccc aaatgaagct
    19441 caacgccaga caggtcgaga ccgcaaagcc aaaagacaaa acctacaaaa tggccgatgg
    19501 tggtggtttg tatcttgagg tttcggccaa aggttccaaa tactggcgca tgaaatacag
    19561 acgtccctct gacaaaaaag aggatcgtct tgcgtttggt gtttggccta ctgtgacgct
    19621 tgctcaggca agagcaaaac gcgatgaagc taaaaagctt ttagtacagg gcatagaccc
    19681 aaaagtcgta caaaaagaag ctcgggccga gaattcgggg gcatatactt ttgaagctat
    19741 cgcccgagaa tggcatgcca gtaacaagcg ctggagtgaa gaccatcgat cgcgcgttct
    19801 tcgctatctt gagctttata tattccctca tatcggttcg tccgacattc ggcagcttaa
    19861 aaccagccac ctgttagccc cgattaaaaa agttgatgcc agtggcaaac acgatgtcgc
    19921 tcagcgcctg caacagcgcg tcacagccat tatgcgttat gccgtacaga acgattacat
    19981 cgactcaaat ccagccagcg atatggctgg tgcgctatcg acaaccaaag cgcgacatta
    20041 ccctgcttta ccttctagcc gattccctga gtttcttgca cgtcttgctg catatcgtgg
    20101 ccgtgtaatg acacggattg cggtcgagct ttccttgcta acttttgtgc gttccagtga
    20161 attacgtttc gcacgttggg atgaattcga cttcgataaa tctctttggc gtatacctgc
    20221 aaagcgagaa gaaattaaag gtgtgcgtta ttcgtaccga ggcatgaaga tgaaagagga
    20281 acatatcgtt ccgcttagtc gacaggcgat gattttatta aaccagttaa agcagattag
    20341 tggtgataaa gagctgcttt ttccggggga tcatgacgca actaaggtta tgagtgaaaa
    20401 cacggtaaac agcgcattgc gtgcgatggg ctatgatact aaaaccgagg tgtgtgggca
    20461 tgggtttagg actatggcgc gtggtgcgtt gggggagtcg gggttatgga gtgatgacgc
    20521 gatagagagg caactgagcc actcagagcg taataatgta cgtgcggcat atatccatac
    20581 ctccgagcat ttggatgagc ggcgtttaat gatgcagtgg tgggctgatt atttggatat
    20641 gaaccgtaat aaatatatta cgccttatga ttattcaaaa tacaaagaaa tacttaaata
    20701 aaaatagtta ttggttgatt tttaaaatga gtgttaagta acataatctg gagtttttaa
    20761 ttgttagttg ctataataaa aatcaacgtg aagtgagtgt gattaacatg gctgagttat
    20821 tgcgagagca ggaagttaaa atagccctaa ttgaatggtt atataaaaaa ggcatgctta
    20881 gtgatgcaac gatcattaat gaaatggttg ttgctaactg gagcagaaga gctgatttgg
    20941 ctattgccaa tggaagccta caagcttttg aaataaaaag cgattttgac tctcttaagc
    21001 gcttggatgg tcagttaaaa atttttaaat ctagcttcga aaaagttaca attgtgtgcg
    21061 cgccaaaatt taccagtgaa gtatctaaac gggtttcatc agatgttggc gttgtagaat
    21121 ttcaatctaa taatagtagc gtacgcttta aaataattca aaaaggaagg gtttgctctc
    21181 gcttaaataa atttgtttat ttgggttttt tgcttaagtc tgagctt
//
LOCUS       DAZFVO020000087        20741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFVO020000087 DAZFVO020000000
VERSION     DAZFVO020000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.087"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            26..550
                     /locus_tag="ACHKNB_RS14760"
                     /old_locus_tag="pgaptmp_002952"
     CDS             26..550
                     /locus_tag="ACHKNB_RS14760"
                     /old_locus_tag="pgaptmp_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HGU4196425.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            634..1320
                     /gene="lpfB"
                     /locus_tag="ACHKNB_RS14765"
                     /old_locus_tag="pgaptmp_002953"
     CDS             634..1320
                     /gene="lpfB"
                     /locus_tag="ACHKNB_RS14765"
                     /old_locus_tag="pgaptmp_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HGU4196426.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQCQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1344..3875
                     /gene="lpfC"
                     /locus_tag="ACHKNB_RS14770"
                     /old_locus_tag="pgaptmp_002954"
     CDS             1344..3875
                     /gene="lpfC"
                     /locus_tag="ACHKNB_RS14770"
                     /old_locus_tag="pgaptmp_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HGU4196427.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLLSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3886..4941
                     /gene="lpfD"
                     /locus_tag="ACHKNB_RS14775"
                     /old_locus_tag="pgaptmp_002955"
     CDS             3886..4941
                     /gene="lpfD"
                     /locus_tag="ACHKNB_RS14775"
                     /old_locus_tag="pgaptmp_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HGU4196428.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4947..5471
                     /gene="lpfE"
                     /locus_tag="ACHKNB_RS14780"
                     /old_locus_tag="pgaptmp_002956"
     CDS             4947..5471
                     /gene="lpfE"
                     /locus_tag="ACHKNB_RS14780"
                     /old_locus_tag="pgaptmp_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HGU4196429.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5724..7415
                     /gene="eptB"
                     /locus_tag="ACHKNB_RS14785"
                     /old_locus_tag="pgaptmp_002957"
     CDS             5724..7415
                     /gene="eptB"
                     /locus_tag="ACHKNB_RS14785"
                     /old_locus_tag="pgaptmp_002957"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HGU4196430.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7507..7583
                     /locus_tag="ACHKNB_RS14790"
                     /old_locus_tag="pgaptmp_002958"
     tRNA            7507..7583
                     /locus_tag="ACHKNB_RS14790"
                     /old_locus_tag="pgaptmp_002958"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7541..7543,aa:Pro,seq:cgg)
     gene            7618..7751
                     /locus_tag="ACHKNB_RS14795"
                     /old_locus_tag="pgaptmp_002959"
     ncRNA           7618..7751
                     /ncRNA_class="other"
                     /locus_tag="ACHKNB_RS14795"
                     /old_locus_tag="pgaptmp_002959"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8496..10103
                     /gene="dppA"
                     /locus_tag="ACHKNB_RS14800"
                     /old_locus_tag="pgaptmp_002960"
     CDS             8496..10103
                     /gene="dppA"
                     /locus_tag="ACHKNB_RS14800"
                     /old_locus_tag="pgaptmp_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HGU4196431.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10254..11273
                     /gene="dppB"
                     /locus_tag="ACHKNB_RS14805"
                     /old_locus_tag="pgaptmp_002961"
     CDS             10254..11273
                     /gene="dppB"
                     /locus_tag="ACHKNB_RS14805"
                     /old_locus_tag="pgaptmp_002961"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HGU4196432.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11283..12185
                     /gene="dppC"
                     /locus_tag="ACHKNB_RS14810"
                     /old_locus_tag="pgaptmp_002962"
     CDS             11283..12185
                     /gene="dppC"
                     /locus_tag="ACHKNB_RS14810"
                     /old_locus_tag="pgaptmp_002962"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HGU4196433.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12196..13179
                     /gene="dppD"
                     /locus_tag="ACHKNB_RS14815"
                     /old_locus_tag="pgaptmp_002963"
     CDS             12196..13179
                     /gene="dppD"
                     /locus_tag="ACHKNB_RS14815"
                     /old_locus_tag="pgaptmp_002963"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HGU4196434.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13176..14180
                     /gene="dppF"
                     /locus_tag="ACHKNB_RS14820"
                     /old_locus_tag="pgaptmp_002964"
     CDS             13176..14180
                     /gene="dppF"
                     /locus_tag="ACHKNB_RS14820"
                     /old_locus_tag="pgaptmp_002964"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HGU4196435.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14210..15481)
                     /locus_tag="ACHKNB_RS14825"
                     /old_locus_tag="pgaptmp_002965"
     CDS             complement(14210..15481)
                     /locus_tag="ACHKNB_RS14825"
                     /old_locus_tag="pgaptmp_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HGU4196436.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15957..16064
                     /locus_tag="ACHKNB_RS14830"
                     /old_locus_tag="pgaptmp_002966"
     CDS             15957..16064
                     /locus_tag="ACHKNB_RS14830"
                     /old_locus_tag="pgaptmp_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196437.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16440..16547
                     /locus_tag="ACHKNB_RS14835"
                     /old_locus_tag="pgaptmp_002967"
     CDS             16440..16547
                     /locus_tag="ACHKNB_RS14835"
                     /old_locus_tag="pgaptmp_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196438.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16923..17030
                     /locus_tag="ACHKNB_RS14840"
                     /old_locus_tag="pgaptmp_002968"
     CDS             16923..17030
                     /locus_tag="ACHKNB_RS14840"
                     /old_locus_tag="pgaptmp_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196439.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17406..17513
                     /locus_tag="ACHKNB_RS14845"
                     /old_locus_tag="pgaptmp_002969"
     CDS             17406..17513
                     /locus_tag="ACHKNB_RS14845"
                     /old_locus_tag="pgaptmp_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196440.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17600..19279)
                     /gene="bcsG"
                     /locus_tag="ACHKNB_RS14850"
                     /old_locus_tag="pgaptmp_002970"
     CDS             complement(17600..19279)
                     /gene="bcsG"
                     /locus_tag="ACHKNB_RS14850"
                     /old_locus_tag="pgaptmp_002970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HGU4196441.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19276..19467)
                     /gene="bcsF"
                     /locus_tag="ACHKNB_RS14855"
                     /old_locus_tag="pgaptmp_002971"
     CDS             complement(19276..19467)
                     /gene="bcsF"
                     /locus_tag="ACHKNB_RS14855"
                     /old_locus_tag="pgaptmp_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HGU4196442.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19464..>20741)
                     /gene="bcsE"
                     /locus_tag="ACHKNB_RS14860"
                     /old_locus_tag="pgaptmp_002972"
     CDS             complement(19464..>20741)
                     /gene="bcsE"
                     /locus_tag="ACHKNB_RS14860"
                     /old_locus_tag="pgaptmp_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HGU4196443.1"
                     /translation="LQCSIDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNH
                     CSLLVINPGNNNDKQFSLLLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQL
                     SVQQQNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEA
                     RTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLAC
                     GANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCN
                     AVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLS
                     FCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQ
                     SSKPVINAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         5238 a         5064 c         5495 g         4944 t
ORIGIN      
        1 tttatctata taaatggagt tataaatgaa aaaggttctg tttgccttat ctgcgcttgc
       61 gctgacctct acttccgtat tcgcagctga tgcgggcgac ggtactgtta aattcaccgg
      121 tgagattgtt gacgccgctt gtgttgtgtc tccagacact caaaaacaag aagttgttct
      181 gggtcaggtg aacaagtctg tatttactgc tatgggtgat aaatccacag caacgccgtt
      241 caaaatcaaa ctggaaaact gcgatatctc cactttcaaa aatgtggaaa tcagctttaa
      301 cggcgttggc gatgcagaca acagcaaact ggtttctgta agcactgaac caggtgccgc
      361 aactggcgta ggtatcggta tttatgataa caccaatacc ctggttgacc tgaatactgg
      421 taaatctgct actgtcctga aagaaggtca gaccgttctg tatttcaccg ctaactatgt
      481 ctctaccaaa gatgcagtaa ccacgggtta cggtaacgct gaagtcgact tcaacctgac
      541 ttacaactaa tcgatcctga cggaacgatc atctctcaat ggcaacagag actgtgttgc
      601 cattattcac accaatgact cagggaccga atcatgaaac gtattagctt tgctgtgctg
      661 gcacttttaa tgaccgcaca acagtgtttt gctggtggcg tagccttaag cacgacgcgt
      721 gttatttatg atggcggcaa gaaagaagcg tcgctgaccg ttgaaaacca taataaaaat
      781 gaagagtttt tgatccagtc atggattgat gatgtgaatg gcaataaaaa aacaccgttt
      841 attattacac cgccattatt caaacttgat cctgataaaa ataacgtctt acgtatcgtc
      901 aatattgaag ataccttacc taaagatcgt gaatccgttt actggattaa tgtaaaggcc
      961 attcctgcga aaagtgctga aaatgaaaat aaaaacgtct tacagatagc cgttcgcacc
     1021 cgtttgaagt tgttctatcg tccaaaaggt ttgccgggaa gcaacctcga ggccatgaaa
     1081 cagttacgtt tccagtgtca aaataaccaa attgtggtta ataacgactc cgcttataac
     1141 ctgaccttta atcagttttc cattaatgga caaaagattg aacgggcggg aatggttctc
     1201 gcgaaaggaa agttaaacat taaccttcca gccggtacag gcaacgcgca cgaagtgaaa
     1261 tacagcatta ttaatgattt cggtgttgct ggcgaaatgc tgacgaagcc gattaactga
     1321 tccttttaca aggcgctttt actatgcatt ggatgcattt acctctctat cactacagag
     1381 cgcatttttc cttctctctg ctggcattaa cgattgccag ctcgttacct gcgtatggcg
     1441 gaaaatttaa ccctaaattt ctcgaaaacg ttcagggtat cgatcaacac atcgacttgt
     1501 cggtatacga ctcacctgtc ggccagcaaa tccccggtaa ataccgggtt tcggtgtttg
     1561 taaacgaaga aaaaatggct tcccggacgc tggattttag taccgccagc gaagcgaaac
     1621 gcaaagccag cggtgagtcg cttatgcctt gccttagccg ggtgcaactg gaagaaatgg
     1681 gcgtgcgcgt tgacagcttt ccggcgctga agatgtcacc accagaagcc tgcgtagcgt
     1741 ttgatgaaat cattccccag gccaccagcc gctttgattt taatactcag acgctgcatc
     1801 tttcctttcc acaggcggca atgatgatga ccgctcgcgg cacggttgat ccttctcgct
     1861 gggacgaagg aatacctgcg ctgttgctgg attacagctt tagtggcagc aatggacgca
     1921 atgaaggtac aggttcatct tcagattcga ccagtgacag ttattacctg aacctgctca
     1981 gcggtttgaa cgttggtccg tggcgtttac gtaataacag tatctggaac cgtaccgatg
     2041 gcaaaaacca atgggataac gttggtacat cgttaaaccg ggcaattatt ccacttaaat
     2101 cacagataac tctgggtgat accgccacgc ctggcgagat ttttgatagc gtacaaatgc
     2161 gtggggcgtt actggcatcc gacgatgaaa tgttgccgga cagccagcgt ggctttgctc
     2221 cggtagtgcg cggcatcgcc aaaagcaacg ctgaagtgag tatcgaacag aacggctatg
     2281 tcatttatcg cacttttgtt cagccgggtg catttgagat taacgatctc tacgcaacct
     2341 ccggcagcgg cgacctgacg gtgatcatta aagaagccga cggaagtgaa caacgtttta
     2401 tccagccgtt ttcggcggtg gcgattttcc agcgtgaagg gtatctgaaa tacagtctgg
     2461 ctgccggtga atatcgtgct ggtaattacg acagcgataa accacatttc gggcagttta
     2521 ccgccatgta cggcttgccg tggggtatga ccgcctacgg tggtgcattg ttgtcggcag
     2581 attacaacgc tttagctctg ggcctgggga aaaacttcgg taccattggc gcagtatcgg
     2641 tggatgtcac tcaggcgaaa agtcagttac gcaacaatga aaaagaggaa ggccagtctt
     2701 atcgcttcct ctattcgaaa agttttgagg gtggcactga cctgcgtctg ttgggctaca
     2761 aatactcaac cagcggctac tacaccttcc aggaagcaac tgatgttcgc agtgacgccg
     2821 acagtgatta ccgccgttat cacaagcgca gccagataca gggcaatatt acccagcaac
     2881 tgggagacta cggctcggtt tatttcaata tgacgcagca ggattactgg aacgttgacg
     2941 gtaaggaaaa ctccttaagc gccggttatc acggtcacat tggtcgggtg aattacagcg
     3001 tggcctatac ctggacgaga agtccggaat gggaagaaga cgatcgcttg tggtcattca
     3061 gtgtttccat ccctctgggg ggcgcgtgga gcagttatcg catgactacc gaccagaatg
     3121 gcaagacgtc gcaacaggcc tctgttagcg gcacgttact ggaagatcgt aacctgagct
     3181 ataacgttca gcagggttac accagtaatg gcgtaggcta cagtggcagc gtcaacatgg
     3241 ggtatatggg tggctctggt aacattgacg taggctataa ctattctaaa gataaccaac
     3301 aggttaacta tggcgtgcgc ggcggtgtca tcgttcacag tgaaggtata acgctttcgc
     3361 agcctttagg cgaaagtctg gcgattgttt ctgcaccggg cgcgcgtggt ggacatgtgg
     3421 ttaacagcag cggcgtagaa gttgactgga tgggcaacgc cgtggtgcct tatctcacac
     3481 catatcgtga aaccattgtg gaattgcgta gcgacacgtt ggggcaaaac gttgaactac
     3541 aggaagcgtt ccagaaagtc gtgcctactc gtggtgcagt tgtgcgttcc cgctttgata
     3601 cccgcgtcgg ttaccgtgtg ctgatgagct taaaacaggc gaatggcaac gcagtaccgt
     3661 ttggtgcaac cgcagcgtta attgatgaga gcaaacctgc cagcagcatt gtgggtgaag
     3721 aaggacagct ttatatcagc gggatgccag aagagggcga attgcaggtg agctggggga
     3781 acgagcaggc gcaacgttgc cgtgtgccat tccgcttgcc agagaacaag gacaatgcgg
     3841 ctatcgtgat ggttaatgcg gtatgcgaga aataaggata tttagatgaa aattaaaaat
     3901 ttgttggcag gtatggtgct gttagggacg agcacttttg ctggaaatat atgggctgcg
     3961 gattggggac catgtcagac atcaaatggg gtggcccatg agtatagttt tgatttcgta
     4021 cagactatcc aggtaccttc agaaaataag gctgggaaaa tattaactca gccctttgct
     4081 ttaggtacaa aatattccgc ttattgtgaa tgcccggatc ccattccaga taatggagtt
     4141 gtgacttatt ttaaaggtgt aacgcttctg cctgagcctg gaactgtaga tgggtactat
     4201 aaattcaata attacattga tattctcacc aaaatatatg tctacacaca attagatatc
     4261 ccggtgcctt ttaccgatcg aagcaatggc acggcacaga aagaatgtac gccctatact
     4321 gctaacaatt gggggacagg tggtaaagga agtatcagta tttatatttc acatcctttt
     4381 gttgggcaaa tgattattcc taaaacacga gttgcgtccc tctttgggac aaaaaagaaa
     4441 ggtgtttaca atgatagtca gcccatggcc aatgtcagta tttccgggag tattacagtt
     4501 actcaaggct gtgaacttgc cgctggcacc gtactggata ttccattcgg agaatatcag
     4561 gcccatgatt ttaaagggcg tgccggacaa ccaccacaaa atgttcagaa ggtacaaaaa
     4621 gaattaagct ttgactgcaa taatatttca gatggcgtca aaatttatct cagtattgat
     4681 gcaacaccaa atactgccta tcccagcgca attgatttag gcaacgccga tgttggtgca
     4741 gtcattgagg acggtaaagg taatattctt aagccgaatg acagtaatag cttattagaa
     4801 atgaacccag gctcgctgta tgaagatgtc aaacgcaaag cgacaaccac aattaccgct
     4861 tatcccgtta gcactaccgg aaaattaccc gctgccgggg attatagcgg tattgctacc
     4921 atacatgtcg aactggagta acagtcatga agcgaacaat acttgcgtta tgcctgctgc
     4981 ctctttcctt ttcgtcattc gcggcaacca cggatttagg gccgaaagga acattgaaat
     5041 tcacccttaa aatcagccag ggtgcttgcg aactggtgaa agacagcgtt gaggttgaca
     5101 tgggcaccgc ggttctcaaa aagccggtaa gaatcggaac ggaaattaat cccactcctt
     5161 tttccatcgg cctgaaaaac tgtagcgaag tggtcagagc ctacgtgact atggacggta
     5221 cacccaatgc agacgactca aatctttttg cgctggatgc gggtggcgct acaggcgttg
     5281 ggctgaaaat caaaaccgct gctggagtac aacagttccc ggaaaataca acgccgacac
     5341 cggttgagtt tgcaattaac ggtgatggcg cacatcaact gaactatatc gccagctacg
     5401 tgcctattcg cgccgatgca actattggtc gcgcagatgc aacggtcgac tttactgtac
     5461 agtacgaata atttcacatc ttaagggcca gttaactggc cctttttact gctttttgta
     5521 acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg cacactcttt
     5581 ccccacactt tttcgctttg ctgtggtcta cttattcgcg cgtgtagatt ttacttatct
     5641 gactacctcc gcacttttcc cctgccgggc ctgaaaagcc gctaagcagg gtgttatcac
     5701 ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag cagaagctga
     5761 gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt tatcgccgct
     5821 tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt ttctgctgtt gttgaactgg
     5881 ccgccaccgt actggtgacc ttctttttac tacgtcttct atcgctgttt ggccgccgca
     5941 gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc aggtgccagc tattacatga
     6001 ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc accgatatcg
     6061 acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc gttagtgcat
     6121 tgcctcttat ccttatctgg aataaccgct gtcgctacac cttgctccga caactgcgaa
     6181 ccccagggca gcgtattcgc agcctggcgg tcgtcgtact ggcgggtatt atggtttggg
     6241 caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgacc ggcgttgatt
     6301 tgccgagtta tggcggtgtc gtagcgaact cttatctgcc atcaaactgg ctttctgcgt
     6361 tggggctgta tgcctgggcg cgggtcgatg aatcttccga taataattca ttgcttaatc
     6421 cggcgaagaa attcacttat caggcaccgc aaaacgttga tgacacttat gtcgtgttta
     6481 tcatcggtga aaccacgcgt tgggaccata tgggtatttt cggctatgag cgtaatacca
     6541 cgccgaaact ggcccaggag aaaaatctgg cggcgttccg tggttactcc tgtgataccg
     6601 caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc
     6661 agcgcacatt aaaagaacag aacattttcg cggttctgaa gcagttagga ttcagttctg
     6721 acctctacgc tatgcagagc gaaatgtggt tctacagcaa cacgatggcg gacaacattg
     6781 cttatcgtga gcagattggt gcggagccac gtaatcgtgg caagccggta gatgatatgt
     6841 tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc ggatggtaag catctgatca
     6901 ttctgcatac caaaggttcg cattttaact acacccagcg ttatccgcgt agcttcgcgc
     6961 agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag atgatcaact
     7021 cctatgacaa ctcggtgacc tatgtggatc actttatctc cagcgtgatt gatcaggttc
     7081 gcgataagaa agcgattgtg ttctacgcag ctgaccacgg tgagtcaatt aatgaacgcg
     7141 agcacctgca cggcacgccg cgtgaactgg caccgccgga gcagttccgc gtaccgatga
     7201 tggtctggat gtcagataaa tatctggaaa atccggccaa tgcgcaggca tttgcgcagc
     7261 tgaaaaaaga agccgacatg aaagtgccac gccgtcacgt agagctgtac gataccatca
     7321 tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac aactggtgtc
     7381 acatcccgca ggcaaaagag gcagcggcta actaagcggc ctgctgactt tctcgccgat
     7441 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
     7501 cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag gggtcggagg
     7561 ttcgaatcct ctatcaccga ccaaattcga aaagcctgct cagcgagcag gcttttttgc
     7621 atttgaagcc catgaggatg agaacctccg ggggctggga ggttcgactc gagcgaagcg
     7681 agagaacgtt gcgccagcaa cggcccgcag ggcgagccac gaagtggcga gtaatcctct
     7741 atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat ctgcagctca
     7801 tgaggatgag aacctccggg ggcagggagg ttcgactcga cgtaggcctg ataagacgcg
     7861 tcagcgtcgc atcaggcaat ggcaccgcac ttattgtcgg atgcggcgtg aacgccttat
     7921 tcgacctaca cgccacacat ttccctgcca aatttgcgcc ccatatctca ctatccatta
     7981 acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt tttttgcgtt
     8041 acttatggct ggcgctggca ttttccgctg tgcgttttag cgttcatggt attaatcgct
     8101 cagatatcca ccttttcctc agattttttt cactaaatgc ataaaactta atcaccaaat
     8161 gttgctaaat attaagctga ttgttctggt catgttggag tagcacaaat ttctctgctg
     8221 caaatagctg tttgaagttt ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat
     8281 cccggttggc tgtattgacg ttttcacatt ctgttgacag attgtaggtc acgaggggca
     8341 ttttatggag gatccgcact gttacactga tgttaattag tacggcatcc ccacctcata
     8401 acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca gcgacaaccc actgcaaagg
     8461 gttaaaacaa caaacatcac aattggagca gaataatgcg tatttccttg aaaaagtcag
     8521 ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta
     8581 aaactctggt ttattgctca gaaggatctc cggaagggtt taacccgcag ctgtttacct
     8641 ccggcaccac ctatgacgcc tcttccgtac cgctttataa ccgcctggtt gaatttaaaa
     8701 tcggcaccac tgaagtgatc ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta
     8761 aaacctatac cttccatctg cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac
     8821 cgacgcgcga actgaacgcc gatgatgtgg tgttctcgtt tgatcgtcag aaaaacgcgc
     8881 aaaacccgta ccataaagtt tctggcggca gctacgaata cttcgaaggc atgggcttgc
     8941 cggagctgat cagcgaagtg aaaaaggtgg acgacaacac cgttcagttt gtgctgactc
     9001 gcccggaagc gccgttcctc gctgacctgg caatggactt cgcctctatt ctgtcaaaag
     9061 aatatgctga cgcgatgatg aaagccggta caccggaaaa actggacctc aacccaatcg
     9121 gcaccggtcc gttccagtta cagcagtacc aaaaagactc ccgtatccgc tacaaagcgt
     9181 ttgatggcta ctggggcacc aaaccgcaga tcgatacgct ggtcttctct attactcctg
     9241 acgcttccgt gcgttatgcg aaattgcaga agaatgaatg ccaggtgatg ccgtacccga
     9301 acccggcaga catcgctcgc atgaagcagg ataaatccat caacctgatg gaaatgccgg
     9361 ggctgaacgt cggctacctc tcctataacg tgcagaaaaa accgctggat gacgtgaaag
     9421 ttcgccaggc actgacctac gcggtgaaca aagacgcgat catcaaagcg gtttatcagg
     9481 gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat gtggggctat aacgacgacg
     9541 ttcaggatta cacctacgat cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg
     9601 aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca gcgcccatat aacccgaacg
     9661 ctcgccgcat ggcggagatg attcaggctg actgggcgaa agtcggcgtg caggccaaaa
     9721 ttgtcaccta cgaatggggt gagtacctca agcgtgcgaa agatggcgag caccagacgg
     9781 taatgatggg ctggactggc gataacgggg atccggataa cttcttcgcc accctgttca
     9841 gctgcgccgc ctctgaacaa ggctccaact actcaaaatg gtgctacaaa ccgtttgaag
     9901 atctgattca accggcgcgt gctaccgacg accataacaa acgcgttgaa ctgtacaagc
     9961 aagcgcaggt ggtgatgcac gatcaggctc cggcactgat catcgctcac tccaccgtgt
    10021 ttgaaccggt acgcaaagaa gttaaaggct atgtggttga tccattaggc aaacatcact
    10081 tcgaaaacgt ctctatcgaa taattaaaag ccatacccgg tggcgctgtg cctctgcgtg
    10141 cagcgccatc cggcagcaat acttcattcc cagcccgacc tgggcgggaa ttgatttgtg
    10201 agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg gttatgttgc
    10261 agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt acccttctca
    10321 catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggcg ggcgaacgtg
    10381 ggatctcccc agagcgtcac gcgcagctgc tggctgaact cggcttagat aaaccgatgt
    10441 ggcagcagta tctccattac atttggggcg ttatgcatgg cgatctaggc atttcaatga
    10501 aaagccgcat cccggtttgg gaagagttcg tgccgcgcct ccaggccacg ctggaacttg
    10561 gcgtctgcgc gatgattttt gcgactgcgg tcggtattcc ggtcggcgtg ctggctgcgg
    10621 ttaaacgcgg ttccattttc gatcacacag cggttggcct ggcgctgacc ggctactcga
    10681 tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac tggaacctga
    10741 cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat ccgttaaccg
    10801 gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc gatgccgtcg
    10861 cccatatgat cttgcctgcc attgtgctgg gtactattcc gctggcggtc attgtgcgta
    10921 tgacacgctc ctcgatgctg gaagtgctgg gcgaggatta catccgcacc gcgcgcgcca
    10981 aagggctaac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg atgctgccgg
    11041 tggtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt ctgaccgaaa
    11101 ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa cgccgcgact
    11161 atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc gtcaacttgc
    11221 tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag taaggggaca
    11281 tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg accccgttac
    11341 aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg gtttacgtcg
    11401 tcatcgtgct gttcatcgcg atctttgcca actggattgc accctataac ccggcggaac
    11461 agttccgcga tgcactgctc gccccgccag cctggcagga aggcggcagc atggcgcact
    11521 tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg cctgatgtac ggtgcgcgcc
    11581 tgtcgctgct ggttggctgt ctggtagttg tgttatcgct gattatgggc gttattctcg
    11641 gcctgatcgc cggttacttt ggcggcctgg tcgataacat cattatgcgc gtggtcgata
    11701 tcatgctggc gctgccaagt ctgctgctgg cgctggtgct ggtggcaatt ttcggcccgt
    11761 cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat gtgcgcttaa
    11821 cccgcgccgc cgtgctggtg gaagttaacc gcgattacgt caccgcgtct cgcgtggcgg
    11881 gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctt gcgccgctga
    11941 ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct cttggtttcc
    12001 tcggcatggg ggcacagccg ccaacgcctg agtggggcac catgctctcc gacgtgttgc
    12061 agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc ctgctgacgg
    12121 tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc aaactgaagc
    12181 agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac
    12241 gaaagcgcgc cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca gggcgaagtg
    12301 gtcgggattg tgggtgagtc cggctccggt aagtcggtca gttcactggc gattatgggg
    12361 ctgattgatt atccgggccg cgtaatggca gaaaaactgg agtttaacgg ccaggatttg
    12421 cagcgtatct cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc gatgatcttc
    12481 caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat tatggaagcg
    12541 attaaggtgc atcagggcgg caacaaaagt acccgccgtc agcgagcgat tgacctgctg
    12601 aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca tcagctttcc
    12661 ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc aaaactgctg
    12721 attgccgatg aaccgaccac cgcgctggac gtgaccattc aggcgcaaat catcgaacta
    12781 ctgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca tgacctggcg
    12841 ctggtggcgg aagcggcaca taaaatcatc gtgatgtatg caggccaggt ggtggaaacc
    12901 ggtgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc attgctgcgt
    12961 gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg tgtcgttccc
    13021 ggtaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta tgccactgac
    13081 agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg atgggcgtca gtccaaatgc
    13141 cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag gccacctcgc
    13201 aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag aaaggcatgt
    13261 tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt gaacgtggca
    13321 aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt cggttgctga
    13381 cgatgattga aatgcccacc ggtggcgagc tgtattacca ggggcaggat ctgcttaagc
    13441 acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc cagaaccctt
    13501 acggttcgct gaatccgcgt aaaaaagttg ggcaaattct cgaagagccg ctgcttatta
    13561 acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg gcgaaagtcg
    13621 gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcgga caacgtcagc
    13681 gtatcgctat tgcccgtggt ctgatgctcg acccggatgt ggtgattgcc gatgagccgg
    13741 tttctgcgct ggacgtctca gtgcgtgcgc aagtgctgaa tctgatgatg gatttacagc
    13801 aggaactggg gctttcttat gtctttatct cccacgacct gtcggtggtg gagcacattg
    13861 ccgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg aaagaccaaa
    13921 tcttcaataa cccgcgccat ccgtacactc aggcgctact ctccgcgacg ccgcgcctga
    13981 acccggacga tcgccgcgaa cgcatcaaac tcaccggtga actgccaagc ccgctgaatc
    14041 caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc tgcacccagt
    14101 tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct gttgatcagg
    14161 atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt tactcaatca
    14221 gcttcaacag tggcgacaag catagcaaaa tgccgtaaag cagaattagc caggctttaa
    14281 atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga atgaggcacg
    14341 acacaatgcc atataacggg cttcccaact gaaagaacac cagcaccgat acacgaaacg
    14401 aaacccaaat cgtcaatgta atgacgataa aagcgcagat cgccagagtc agcatgcgtg
    14461 agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg cctttaatag
    14521 cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag atattcagca
    14581 ccgtagaggt gatatgaatg atatgtccag ggatcacctg cgcggccagc gccagtgccg
    14641 agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa
    14701 aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg gtacgcagcg
    14761 cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata ttcattgggt
    14821 taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc aagacatcgc
    14881 ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca
    14941 tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc ccggaaatct
    15001 taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa atagcgactt
    15061 tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc aggtacgagg
    15121 cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt
    15181 agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt
    15241 agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc actacccagg
    15301 tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt
    15361 gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc
    15421 cgaagtcgta gcgtgtaaac ggcaatttct gattgtgttt cgatagtgtg ttgtgctgca
    15481 taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag tttctcttgc
    15541 gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg
    15601 gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt ctggagtcag
    15661 cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc atcgcgcaat
    15721 gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg caagccgaaa
    15781 gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag
    15841 agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg ctaatcttga
    15901 acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc ggctatatga
    15961 cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc attgctggca
    16021 ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc atgtgggcgt
    16081 caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa ctctgcggcc
    16141 tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa tgcgcagcac
    16201 agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag cgtcaagccg
    16261 gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca ttggttgctg
    16321 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg gggccattct
    16381 tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca ggcggctata
    16441 tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg gtcattgctg
    16501 gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt gccatgcggg
    16561 cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcaggggtg aaactctgcg
    16621 gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag
    16681 cacagagtga agacgtgacg gattccggaa cagaaaaaca gtcgtagaat cagcggcaag
    16741 ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg
    16801 ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca cgggggccat
    16861 tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac acaggcggct
    16921 atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct ccggtcattg
    16981 ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa cgtgtcatgc
    17041 gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg gtgaaactct
    17101 gcggcctttt tcgtaagaaa aatgcaacgg ttgcaggcgg ataatattct gcggaatgcg
    17161 cagcacagag tgagtaagtg atggatttcg gaacagaaaa acagtcgtag aatcagcggc
    17221 aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    17281 ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca taacgggggc
    17341 taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttgtcattac aacacaggcg
    17401 gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagcg gctccggtca
    17461 ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag taacgtgtca
    17521 tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca gaggttaaac
    17581 tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg ttcaggcgaa
    17641 cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag accggtgctg
    17701 tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg aaaatcttgc
    17761 catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt tggtcaatga
    17821 caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca acggggacgt
    17881 ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg tcgcccttca
    17941 gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat ttctccagtt
    18001 cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg taatccgctg
    18061 ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg tagaacgtgg
    18121 cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc acggcggtat
    18181 cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt gtttgatcca
    18241 tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg ccgaactggc
    18301 cgttatgccc catcatcagg tgctgggtaa agcccagttt cgaaaggtta tcaaacagat
    18361 agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg cagctggcgc
    18421 gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag ttcttgaact
    18481 caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct atatccgacc
    18541 aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca gcgggcagcg
    18601 aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc agccaggcgt
    18661 taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact accggtgcgc
    18721 cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca gtggtcgtcg
    18781 gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg ttcagccata
    18841 gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat aaccaggcca
    18901 ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg acaaggtcga
    18961 ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg ctttccgggc
    19021 caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca atccagtggc
    19081 gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgcggca aacaccaaat
    19141 tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta accagaaaat
    19201 agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc atggcggtat
    19261 tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc gtaacgtccc
    19321 ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc gaatgcgtcg
    19381 caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa cgacaataat
    19441 ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc taacagtcgc
    19501 atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat gaccggttta
    19561 gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa gcgcatctgc
    19621 accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga gaaaatgtcg
    19681 ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat ccgacagaat
    19741 gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt acggttaggg
    19801 cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag ggcaaccaga
    19861 acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac ggcattacag
    19921 aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga cagcaaggta
    19981 gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc gatcatcgtc
    20041 agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca ggccaataac
    20101 aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat tttcatcgca
    20161 ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat ttgcgcattt
    20221 tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt gaacaggact
    20281 tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc cagtacagca
    20341 acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc ttcgctttga
    20401 actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc gctgacccct
    20461 ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa acgcaaactg
    20521 gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt atcgttatta
    20581 tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt attcatttta
    20641 tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt tgcgcaaaca
    20701 agaataaaaa ggtaattatg gggatcaata gaacactgca a
//
LOCUS       DAZFVO020000088        20669 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFVO020000088 DAZFVO020000000
VERSION     DAZFVO020000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.088"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..309)
                     /locus_tag="ACHKNB_RS14865"
                     /old_locus_tag="pgaptmp_002973"
     CDS             complement(<1..309)
                     /locus_tag="ACHKNB_RS14865"
                     /old_locus_tag="pgaptmp_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4196444.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     I"
     gene            complement(380..2494)
                     /gene="fusA"
                     /locus_tag="ACHKNB_RS14870"
                     /old_locus_tag="pgaptmp_002974"
     CDS             complement(380..2494)
                     /gene="fusA"
                     /locus_tag="ACHKNB_RS14870"
                     /old_locus_tag="pgaptmp_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGU4196445.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2591..3061)
                     /gene="rpsG"
                     /locus_tag="ACHKNB_RS14875"
                     /old_locus_tag="pgaptmp_002975"
     CDS             complement(2591..3061)
                     /gene="rpsG"
                     /locus_tag="ACHKNB_RS14875"
                     /old_locus_tag="pgaptmp_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGU4196446.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3158..3532)
                     /gene="rpsL"
                     /locus_tag="ACHKNB_RS14880"
                     /old_locus_tag="pgaptmp_002976"
     CDS             complement(3158..3532)
                     /gene="rpsL"
                     /locus_tag="ACHKNB_RS14880"
                     /old_locus_tag="pgaptmp_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGU4196447.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3658..3945)
                     /gene="tusB"
                     /locus_tag="ACHKNB_RS14885"
                     /old_locus_tag="pgaptmp_002977"
     CDS             complement(3658..3945)
                     /gene="tusB"
                     /locus_tag="ACHKNB_RS14885"
                     /old_locus_tag="pgaptmp_002977"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HGU4196448.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3953..4312)
                     /gene="tusC"
                     /locus_tag="ACHKNB_RS14890"
                     /old_locus_tag="pgaptmp_002978"
     CDS             complement(3953..4312)
                     /gene="tusC"
                     /locus_tag="ACHKNB_RS14890"
                     /old_locus_tag="pgaptmp_002978"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HGU4196449.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4312..4698)
                     /gene="tusD"
                     /locus_tag="ACHKNB_RS14895"
                     /old_locus_tag="pgaptmp_002979"
     CDS             complement(4312..4698)
                     /gene="tusD"
                     /locus_tag="ACHKNB_RS14895"
                     /old_locus_tag="pgaptmp_002979"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HGU4196450.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4698..5420)
                     /gene="yheO"
                     /locus_tag="ACHKNB_RS14900"
                     /old_locus_tag="pgaptmp_002980"
     CDS             complement(4698..5420)
                     /gene="yheO"
                     /locus_tag="ACHKNB_RS14900"
                     /old_locus_tag="pgaptmp_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4196451.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5587..6399)
                     /gene="fkpA"
                     /locus_tag="ACHKNB_RS14905"
                     /old_locus_tag="pgaptmp_002981"
     CDS             complement(5587..6399)
                     /gene="fkpA"
                     /locus_tag="ACHKNB_RS14905"
                     /old_locus_tag="pgaptmp_002981"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4196452.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6620..6838
                     /gene="slyX"
                     /locus_tag="ACHKNB_RS14910"
                     /old_locus_tag="pgaptmp_002982"
     CDS             6620..6838
                     /gene="slyX"
                     /locus_tag="ACHKNB_RS14910"
                     /old_locus_tag="pgaptmp_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HGU4196453.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMEKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6887..7477)
                     /gene="slyD"
                     /locus_tag="ACHKNB_RS14915"
                     /old_locus_tag="pgaptmp_002983"
     CDS             complement(6887..7477)
                     /gene="slyD"
                     /locus_tag="ACHKNB_RS14915"
                     /old_locus_tag="pgaptmp_002983"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGU4196454.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7572..7772)
                     /gene="yheV"
                     /locus_tag="ACHKNB_RS14920"
                     /old_locus_tag="pgaptmp_002984"
     CDS             complement(7572..7772)
                     /gene="yheV"
                     /locus_tag="ACHKNB_RS14920"
                     /old_locus_tag="pgaptmp_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HGU4196455.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7782..9587)
                     /gene="kefB"
                     /locus_tag="ACHKNB_RS14925"
                     /old_locus_tag="pgaptmp_002985"
     CDS             complement(7782..9587)
                     /gene="kefB"
                     /locus_tag="ACHKNB_RS14925"
                     /old_locus_tag="pgaptmp_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HGU4196456.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9587..10138)
                     /gene="kefG"
                     /locus_tag="ACHKNB_RS14930"
                     /old_locus_tag="pgaptmp_002986"
     CDS             complement(9587..10138)
                     /gene="kefG"
                     /locus_tag="ACHKNB_RS14930"
                     /old_locus_tag="pgaptmp_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HGU4196457.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10269..12182
                     /gene="yheS"
                     /locus_tag="ACHKNB_RS14935"
                     /old_locus_tag="pgaptmp_002987"
     CDS             10269..12182
                     /gene="yheS"
                     /locus_tag="ACHKNB_RS14935"
                     /old_locus_tag="pgaptmp_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4196458.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12182..13204
                     /gene="yheT"
                     /locus_tag="ACHKNB_RS14940"
                     /old_locus_tag="pgaptmp_002988"
     CDS             12182..13204
                     /gene="yheT"
                     /locus_tag="ACHKNB_RS14940"
                     /old_locus_tag="pgaptmp_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HGU4196459.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13198..13416
                     /gene="yheU"
                     /locus_tag="ACHKNB_RS14945"
                     /old_locus_tag="pgaptmp_002989"
     CDS             13198..13416
                     /gene="yheU"
                     /locus_tag="ACHKNB_RS14945"
                     /old_locus_tag="pgaptmp_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HGU4196460.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13470..14339
                     /gene="prkB"
                     /locus_tag="ACHKNB_RS14950"
                     /old_locus_tag="pgaptmp_002990"
     CDS             13470..14339
                     /gene="prkB"
                     /locus_tag="ACHKNB_RS14950"
                     /old_locus_tag="pgaptmp_002990"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HGU4196461.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14394..14798)
                     /gene="yhfA"
                     /locus_tag="ACHKNB_RS14955"
                     /old_locus_tag="pgaptmp_002991"
     CDS             complement(14394..14798)
                     /gene="yhfA"
                     /locus_tag="ACHKNB_RS14955"
                     /old_locus_tag="pgaptmp_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HGU4196462.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15100..15732
                     /gene="crp"
                     /locus_tag="ACHKNB_RS14960"
                     /old_locus_tag="pgaptmp_002992"
     CDS             15100..15732
                     /gene="crp"
                     /locus_tag="ACHKNB_RS14960"
                     /old_locus_tag="pgaptmp_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HGU4196463.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15783..17873
                     /gene="yhfK"
                     /locus_tag="ACHKNB_RS14965"
                     /old_locus_tag="pgaptmp_002993"
     CDS             15783..17873
                     /gene="yhfK"
                     /locus_tag="ACHKNB_RS14965"
                     /old_locus_tag="pgaptmp_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HGU4196464.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLANY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17940..19160)
                     /gene="argD"
                     /locus_tag="ACHKNB_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /gene_synonym="dapC"
     CDS             complement(17940..19160)
                     /gene="argD"
                     /locus_tag="ACHKNB_RS14970"
                     /old_locus_tag="pgaptmp_002994"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HGU4196465.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19246..19809)
                     /gene="pabA"
                     /locus_tag="ACHKNB_RS14975"
                     /old_locus_tag="pgaptmp_002995"
     CDS             complement(19246..19809)
                     /gene="pabA"
                     /locus_tag="ACHKNB_RS14975"
                     /old_locus_tag="pgaptmp_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HGU4196466.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVELGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19841..20443)
                     /gene="fic"
                     /locus_tag="ACHKNB_RS14980"
                     /old_locus_tag="pgaptmp_002996"
     CDS             complement(19841..20443)
                     /gene="fic"
                     /locus_tag="ACHKNB_RS14980"
                     /old_locus_tag="pgaptmp_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HGU4196467.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYKL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20433..20600)
                     /gene="yhfG"
                     /locus_tag="ACHKNB_RS14985"
                     /old_locus_tag="pgaptmp_002997"
     CDS             complement(20433..20600)
                     /gene="yhfG"
                     /locus_tag="ACHKNB_RS14985"
                     /old_locus_tag="pgaptmp_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HGU4196468.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         5069 a         5654 c         5127 g         4819 t
ORIGIN      
        1 gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc
       61 cgggcagtct acgtgtgcgt agtgacgggt cggggtgtca tattcaacgt gagaagtgtt
      121 gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga atgcacgagc
      181 agcaccgccg taggttttag ccagtacggt ggtgattgca gcggtcagag tagttttacc
      241 gtggtcaacg tggccgatag taccaacgtt aacgtgcggt tttgtacgtt caaatttttc
      301 tttagacacg gctatattcc ttactatagt gctctcccct tcaggagaga gcacgggact
      361 ttggtattaa cccttaggct tatttaccac gggcttcaat tacggcctga gcaacgttac
      421 tcggcgcttc atcatacttc aggaattcca tagtgtatga tgcacgacct ttggtcagag
      481 aacgcagctg agttgcgtat ccgaacattt cagacagcgg tacttcagcg tggatcttaa
      541 cgccagtaac ttcagattcc tgacctttga gcataccacg acgacggctc aagtcaccga
      601 taacgtcacc ggtgttctct tccggagttt ctacttcaac cttcatgatc ggctcaagca
      661 gaactggttt cgctttctta aagccttctt taaaggcgat agaagcagcc agtttaaacg
      721 ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg cagacgaata cccatgtcta
      781 ctaccgggta gcctgccagc ggacctgctt tcagctgttc ctggatacct ttatcaacgg
      841 ccgggatgta ttcgccaggg attacaccac ctttaatgtc gttgatgaac tcgtagcctt
      901 tcgggtttga acccggctcc agcgggtaca tgtcgataac aacatgacca tactgaccac
      961 gaccaccaga ctgtttcgcg tgtttacctt caacatcggt aactttctgg cggatagttt
     1021 cacggtaagc aacctgcggt ttacctacgt tcgcttcaac gttgaattca cgcttcatac
     1081 ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc gataatggtc tggttagatt
     1141 cttcgtcagt ccatacacgg aaagacgggt cttctttagc cagacggccc agagccagac
     1201 ccattttttc ctggtcagct ttggttttcg gttcaactgc gatggagatt accggctcag
     1261 ggaattccat acgttccaga atgatcggcg catccgggtc acacagggtg tcaccagtgg
     1321 ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc gcgaacttct ttgatctctt
     1381 cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg ctcacgtgca gctttcacgg
     1441 agttcagtac ggtatcacca gagttaacca caccggagta aacacggaag aaggtcaggt
     1501 taccaacaaa cgggtcggta gcgattttga acgccagtgc agagaacggc tcgtcatcac
     1561 ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag gataccgttg atcgcaggta
     1621 cgtcaaccgg ggatggcagg taatcaatta ccgcatccag catcgcctga acacctttgt
     1681 tcttgaacgc agaaccacag gttaccagga tgatttcgtt gttcagaacg cgctgacgca
     1741 gagcaccttt gatttctgct tcagtcagtt cttcaccacc caggtatttt tccatcagct
     1801 cttcagaagc ttcagctgcg gattcgatca ggttctggtg ccattcgtta gccagttcaa
     1861 ccatgtctgc cgggatatct tcgtattcga aggttacgcc ctggtcagcg tcgttccagt
     1921 tgatagcttt cattttcacc aggtcaacaa caccggtgaa atgttcttca gcaccaatcg
     1981 ccagctgcag cggaaccggg ttcgcgccca gacgggtttt gatctggtta acaactttca
     2041 ggaagttcgc acccatgcgg tccattttgt taacgaacgc aatgcgcgga actttatatt
     2101 tgtttgcctg acgccatacg gtttcagact gcggctgaac accaccaact gcgcagtaaa
     2161 ccattaccgc accatcgaga acacgcatgg aacgttctac ttcgattgtg aagtcaacgt
     2221 gccccggggt gtcgatgatg ttgatgcgat gcggctcata ctgcttagcc ataccagacc
     2281 agaatgcagt agtcgcagcg gaagtgatgg taataccacg ttcctgctcc tgctccatcc
     2341 agtccatggt tgcagcgccg tcatgaactt caccgatttt atggtttaca ccggtgtaga
     2401 acagaatacg ttcggtagta gtggttttac cggcgtcgat gtgcgcactg ataccgatat
     2461 tacggtagcg tgcgatgggt gttgtacgag ccatttgttt cctcgtttat cttttaggcg
     2521 ttcaatttaa gtagcccaaa gcgggctgct tactggaagc gcccgcctgg tgactaaaac
     2581 tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt ggcttcggcc atacggtgaa
     2641 cgtcttcacg tttcttaact gcagtacctt tgttttctgc agcatcagaa agttcgttcg
     2701 ccaggcgcag agccatggat ttatcaccgc gtttacgagc agcttcaacg atccaacgca
     2761 ttgccagagc attacgacga accggacgga cttcaactgg tacctgataa gtagaaccac
     2821 caacgcggcg agacttaact tctacagtcg ggcgcacgtt ttcgagagct acttcgaatg
     2881 cttccagttc agatttacca gagcgctgag ccagggtctc cagcgcgctg tatacgatag
     2941 attcagcagt agatttttta ccatctacca tcaggatatt tacaaattta gccagcagtt
     3001 ctgatccgaa cttcggatcc ggcagaattt tacgctgacc aatgacgcga cgacgtggca
     3061 tggaaatact ccgttgttaa ttcaggattg tccaaaactc tacgagttta gtttgacatt
     3121 taagttaaaa cgtttggcct tacttaacgg agaaccatta agccttagga cgcttcacgc
     3181 catacttgga acgagcctgc ttacggtctt taacgccgga gcagtcaagc gcaccacgta
     3241 cggtgtggta acgaacaccc gggaggtctt taacacgacc gccacggatc aggatcacgg
     3301 agtgctcctg caggttgtga ccttcaccac cgatgtagga agtcacttcg aaaccgttag
     3361 tcagacgaac acggcatact ttacgcagcg cggagttcgg ttttttagga gtggtagtat
     3421 atacacgagt acatacgcca cgtttttgcg ggcatgcttc cagcgcaggc acgttgcttt
     3481 tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt aactgttgcc attaaatagc
     3541 tcctggtttt agcttttgct tcgtaaacac gtaataaaac gtcctcacac aatatgagga
     3601 cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat atacaacgat cccgccatca
     3661 ccaggccatc tggctggagt gcttaaccgt aagtctgacg aaatcagtat agtcaatggg
     3721 aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca ataaggtctt cgttcagggc
     3781 atagacctta atgggggcat tacgcagact ttcaaggtag cggttaccgt caactgcggc
     3841 agttacgcca tcttgcaata atagcagttc gtctccttca ctgagcagac gcagcagcgc
     3901 agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc agcataaacg cctcaaaacc
     3961 tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc atctgcttcg agcggcgtgg
     4021 cttcgacaac aaagggtgtc tgcggatcta acccgcgttc gcgcagtgaa gccgcacaaa
     4081 cccagcactg ttcaatgtcg tacagaccca acaatttaaa agtggcaatg taatcacgcg
     4141 ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa aacgccatca gcaataaaga
     4201 agacggccag atcgtcagtt aatgcggaag ttgccagtaa agcatctaaa ccttcccgac
     4261 cagcagctgt accatgaggt gcagtagaaa aaacaaacgc aattcgtttc atcagaactg
     4321 taccaccctg tcacaggtca gcgaggcttc cgccagcgcg ccaagtccgc ttaaggtaaa
     4381 tcccggctga aggtttgacg aagccagccc cagccttcct gcttcagttt catcgacgat
     4441 accgcggcgt aacgctgccg ctacgcagat attcagcgcc acaccatgtt gcgcattcaa
     4501 ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc ggtgaggtca attggttagc
     4561 gttatagacc ccttcccgat agaaaaagac gctgcttaac tcatggcctt ctactatcag
     4621 cgcctgcgca aactgaaaag cactactcgc ctgttgcgta ccgtatgctg gcccggtcac
     4681 cacgatggca aaacgcatta cttatcttgc ccctggaaat caccgctttt gaactggcgg
     4741 atgtagagat agacagtgtg tttggagatg ttcaggcggt cagcaacctg gttgatcgcg
     4801 tctttaatat cgaagatccc tttctcgtag agattcagca cgatctgacg atttttggcg
     4861 ttattagaaa cattgcgatc ggcattcact tcttcgatgg tgaactccag cgtttgggta
     4921 accagatctt caacagaaga ggcaaagttg acgcttgaac cgacatccgg ggtttctggc
     4981 ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa gattcatatt gatgcacagc
     5041 agaccaatta cacgctgttc gcggttacga atcgcgatag tcagggactt cattaatacg
     5101 ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc tatccgctcc cgtcatatcg
     5161 tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa tcttccggcc tgtatgttca
     5221 ccgttagcaa tgcgaatggc tgaacatttt agatcctgca aagagtgcaa aacgatttca
     5281 cagtgggagc caataagcat cgctaacccg tccaccaccg cttcgtagga tttcagaata
     5341 tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat ccaactcact ggtttcgttg
     5401 gttaaaagcg acctggacat gaaaaaaaac actccttttc aggagcctgt cgttaacttt
     5461 tcagggcagg ctcattaatg atgcgggtaa ctaaattaat acagcggagg ttccgctttc
     5521 cagcactaat tatatccggc ctgtaataaa aaaaccgccg cctggtcagg cggcggttct
     5581 taatgcttat tttttagcag aatctgcggc tttcgcatca gcttccggct ttgcatcagc
     5641 cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac accagggtag aattcggtgg
     5701 gatccccgga acacccgctt tgccgtaagc cagttctggt ggaataacca gtttgatctt
     5761 accgcctttc ttgatgttct tcagaccttc tgtccaaccc gggataacac cgtccagacg
     5821 gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac tctttaccgt cgatcagagt
     5881 acctttgtag ttcacgacaa cagtatcgct gtctttcggt gcttcgcctt taccggcttc
     5941 tactacctga taaaccagac cagttgaaga ggttttcaca cctttctctt tggcaaattt
     6001 ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg tctttttcca tcttcgcctg
     6061 agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc tgttcgatct cttggtcgga
     6121 gagtttgctc ttatcagcaa atgcatcctg aacaccagcg atcagctgat ctttatccag
     6181 tttgatgccc agtttttctt gttcttttag agagttttcc atgtaacgac ccagcgaggc
     6241 acccagtgca taagctgact tctgatcgtc atttttgaac gctgctttgc tgtcagcagt
     6301 tgtagcaggt tttgcagctt cagcagcaaa agtgattggt gcatgcaggg caacggccat
     6361 tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc catatctcca ggatcggggc
     6421 atctcacccc agggttaact attatcagaa gggtactata aagcgttgtc gaacaaatct
     6481 acatacagac acgccctatt atcatctatt ttcagactct ttttgtttaa attagtttcg
     6541 atgaccgcga aatgagtgct gtctcgggca gcaaagttaa gtagaatccg cggcaaccat
     6601 tcgacaaaag aggtgaatca tgcaggattt atcattggaa gcacgcctgg cagagctgga
     6661 gagccgactg gcttttcagg aaatcaccat tgaagaactg aacgtcacgg tgaccgctca
     6721 tgaaatggag atggagaaac tgcgcgatca tctgcgtctg ctgactgaga agttaaaagc
     6781 cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg ccaccgccgc attattgagg
     6841 cgaaaaaaaa gcggggaatc cccgcttttt tgtcaccttt tcggtattag tggcaaccac
     6901 aaccgccgtt gcctttaccg ccacagcagc cttcgccacc gtgttcatga ccgtgatcgt
     6961 ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg gtggtgatcg tgcgcgccgt
     7021 gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg aatcgccaca acttcaacgt
     7081 tgaatttcag gttctgaccg gccagcatgt ggttaccatc aaccacgacg tgatcgtctt
     7141 caaccgcagt gatttcaacc ggtaccggac cctggtcagt ttcagccagg aaacgcatac
     7201 ctacctgcag ttcatcaacg cccataaata cgtctttagg aacacgttgc accaggtttt
     7261 cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac atcaaatttg tcgccaactt
     7321 catgaccttc cagcgccgtt tccaggccag agatcaggga accgtgacca tgcaggtagt
     7381 ccagcggcgc actcaccgga gactcatcaa ccaacacacc gtcttctgta cgtacctgat
     7441 aggccaggct gaccaccagg tcttttgcta ctttcatgat atctcctgag catgggaaga
     7501 atagtggcgc agattgtagc ggaattctgc agccgtgtac tcactagctt aaaaaaactc
     7561 ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt gctcatcttt gcgaacgtga
     7621 tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc acttaacgca ttcaacaata
     7681 tcaatattat tttcgcgcca catcgccatt gaatcctgcg cctgacaggc cgggcatttt
     7741 gcgcccgcaa taaaacgttt tcggattgcc atctttaccc tctactcaaa ttcatcccag
     7801 ccgtccagct ggcgtcgttc ttgttgcatt tcacgttgga aaatctcttc cagttcgcgt
     7861 cgggcttccc tggcgcgaga aatttgtacg gtatcagcat gcatcgggat taactcccgc
     7921 agcattcgca tatccaggcg gcgaaaatgc agttgcgccc gctgtgcctg atgcggatgc
     7981 atgccaagcg tgaccagcgt cttgcgcccc agctctaacg cactggagaa tgtttcacgg
     8041 gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt ccacacgtcc gcgcgctcgc
     8101 gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt ccaccagctt catggtgtct
     8161 tccggctcgt tacaggtaat gacgatagac tcagcggcct ctgcacccgc agaacgtaaa
     8221 agatcgacct gcgtggcgtc gccgtaataa actttgtagc catatttacg catcaggtta
     8281 acggcgctga tatcccgctc cagcacggta atgcgcattt tatttgccat cagcaaacga
     8341 ccaatcacct gaccaaaacg cccgaagccc acgacaatga cctggggttt atcatcgttg
     8401 acccacggtt tttcgtcttc ttcttccggt ccgttaaact ggcgggatag ccatttatcc
     8461 accagcttca tcagcagcgg cgtggtcatc atggaaagcg tcaccgtcac cagcaacagc
     8521 gccatctggt cgccctggaa taagcgttgt gaagaagcgg tagaaaagag gacaaaggca
     8581 aactcaccgc cctgactcaa cacgccagca aactgcatcc gctctgagct acgcacgcca
     8641 tacaatcgcg ccagcagata caacacgaga attttcaccg ccaccagcac aaccacgctt
     8701 atcactaccc acaacagatg ggtataaagc accccgaggt tgagcgacat gccgacagag
     8761 ataaagaaca aaccgagcag caagcctttg aagggatcga tagccgtttc cagttcatgg
     8821 cgatattcac tttccgccag cagcacgccc gcaataaacg taccgagcgc catcgacagc
     8881 cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca gcagcgtcgc ggcggtgaac
     8941 acttcccgca cgccagaagc tgcaataaaa cggaataccg gacgcagtaa atagcgcccg
     9001 ccaatcagca tgccgacaaa cgccagcacc ttcatgccga tcttcatcca gtcgaaatgt
     9061 tcgtctgccg aacccgccaa caacggcact aacgccagtg ccgggatcac cgccagatcc
     9121 tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc tgcgattcat ccctttctca
     9181 cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa ggccaatgcc accgacaacc
     9241 gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg ccagcaacgc cgcgcttaac
     9301 agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca gttgccaaag tttggaggga
     9361 ttcaactcaa gaccgatgat aaacatcagg aataccacgc cgagttccga aaagtggagg
     9421 atctcatcga cgtcgctaat aaaccccagc ccccatgggc caattgcaat ccctgccagc
     9481 aaatatccca acacagcgcc aatacccagc cgcgatgcca gcggcaccgc agccaccgcc
     9541 gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac cttccatcag cggcctcctg
     9601 gagacagcgg atttgccagc cagtcaccgt aggctctggc gtgactcgcc agctcctgtg
     9661 cgctttgccg tctcgcccag taaataatga tgggacttaa ccaatgcatc cggcacatgc
     9721 ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg gtagcgattc aatgcgtcat
     9781 aacggtaagc actttccggc tcgccggtgg taatcacgct acgccagtac tttcccgcca
     9841 gttggtttcc tcccggcccg ctggcaaaac cacgacttaa tacccggtcc agccactctt
     9901 tcagtagcgc cgggcagcta taggtataaa gaggatgctg aaagacaatc acctcgtgct
     9961 cgcgcagtaa tgcctgctca cgggggatat caataaaaaa atcgggatag tgcgcataaa
    10021 ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt aagcagtacc cggtttgcca
    10081 ccgagtcctg agattccgga tgggcataca gcagcaaaac tttcgctggc tgagacatca
    10141 tccccctccc ggtatggttt ttgtgtatag tcgctgtttt gggctaccat tgcgcccggt
    10201 gcggcagctc gcccatacat tacattatca taatgataag ttaacatagt ctgaacatac
    10261 ggcaccttat gattgttttc tcctcgttac aaattcgtcg cggcgtgcgc gtcctgctgg
    10321 ataatgccac cgccaccatc aaccctgggc agaaagtcgg cctggtgggt aaaaacggct
    10381 gtggtaaatc taccctgctg gcattgctga aaaatgaaat cagcgccgac ggcggcagct
    10441 acacctttcc gggaagctgg caactggcgt gggtgaatca ggaaacgccg gcgttaccgc
    10501 aagcggcgct ggaatatgtc attgacggcg accgtgaata tcgtcaacta gaagcgcagc
    10561 tacacgacgc caacgaacgt aacgacgggc acgccattgc gaccattcat ggcaagctgg
    10621 atgctattga cgcatggagt attcgctccc gtgccgccag cctgctgcac ggcctcggtt
    10681 tcagcaatga acaactggag cgcccggtaa gtgatttctc cggtggctgg cgtatgcgtc
    10741 ttaaccttgc ccaggcgctg atttgccgtt cagacttgct gctgctcgac gaaccgacta
    10801 accacctcga tctcgatgcc gttatctggc tggaaaaatg gttgaagagc tatcagggca
    10861 cgctgatcct gatctctcac gaccgcgact tcctcgatcc gatcgttgat aaaattattc
    10921 atatcgaaca acaaagcatg ttcgagtaca ccggcaacta cagttcgttt gaagtacagc
    10981 gcgccacccg tctggcgcag caacaagcga tgtatgaaag ccagcaggaa cgcgtagcgc
    11041 atctgcaaag ttatatcgac cgtttccgtg ccaaagccac caaagcgaag caggcccaga
    11101 gccgcattaa gatgctcgag cgtatggagc tgattgcccc cgcgcacgtc gacaacccgt
    11161 tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc gttactgaag atggaaaaag
    11221 tcagcgcggg ctatggcgat cgcattattc tcgactcgat taaactgaac ctggtgcccg
    11281 gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa atcgacatta atcaaactgt
    11341 tagccggtga acttgcgcca gtcagcggtg aaattggtct ggcgaaaggg atcaagctcg
    11401 gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga cgaatcacct attcaacatc
    11461 tggcacgttt agcgccgcag gagctggaac aaaaactgcg tgactacctc ggcggctttg
    11521 gtttccaggg cgataaagta accgaagaaa cgcgccgctt ctcaggtggg gaaaaagccc
    11581 gcctggtgct ggcattaatt gtctggcagc gtccgaatct gctgctgctc gacgaaccga
    11641 ctaaccacct tgacctcgac atgcgtcagg cactcaccga agcattaatc gagttcgaag
    11701 gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg ttccaccact gacgacctct
    11761 acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga tctggaagat tatcaacagt
    11821 ggttgagcga cgtacaaaag caggaaaacc agaccgacga cgcgccaaaa gagaatgcga
    11881 acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc ggagctgcgt gcgcaaaccc
    11941 agccactgcg taaagagatt gcccgtctgg aaaaagagat ggagaagctg aacgcgcaac
    12001 tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta tgaccagagc cgtaaagcgg
    12061 agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc cggcctggaa gagtgcgaaa
    12121 tggcgtggct ggaagcccag gagcagcttg agcagatgct gctggaaggc caaagcaact
    12181 gatggcgcag ataacgacga ccgatgccaa tgaattcagc agcagtgctg aattcatccc
    12241 catgcgcggc tttagcaact gccatctaca aactatgctg ccgcgtctgt ttcgtcgtca
    12301 ggtgaaattc accccgtact ggcagcggct ggagttgccc gacggcgatt ttgtcgatct
    12361 tgcgtggagt gaaaaccctg cacaggcgca acataaacct cgtctggtgg tgtttcacgg
    12421 gctggaaggc agtctcaata gcccttacgc ccacggtctg gttgaggcgg cgcaaaagcg
    12481 cggctggctg ggcgtggtga tgcattttcg cggatgcagc ggtgaaccaa accgtatgca
    12541 ccgcatttac cattcgggcg aaaccgaaga cgccagttgg tttttacgct ggctgcaacg
    12601 cgaatttggt catgcgccaa cggctgccgt cggctattcg ctcggcggta atatgctggc
    12661 ctgtttgctg gcaaaagaag gcaatgatct ccctgttgat gcggcggtga ttgtctctgc
    12721 gccgtttatg ctggaagcct gtagctatca tatggaaaag ggcttttccc gcgtttatca
    12781 gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc aagctggcag cctaccccgg
    12841 aacactgccg attaatctcg cgcagttaaa atcggtacgt cgcatccgtg aatttgacga
    12901 tctaatcacc gccagaattc acggctacgc tgacgctatc gactattatc gtcagtgtag
    12961 cgccatgccg atgctgaacc ggatcgccaa acctacgctg attattcacg ccaaagacga
    13021 tccgtttatg gatcatcagg tgatcccgaa accggaaagt ctccccccgc aggttgagta
    13081 tcaactgact gaacatggcg gtcatgttgg ctttattggc ggtacattac ttcatccgca
    13141 aatgtggctg gagtcacgca ttcctgactg gttaacaacg tatctggagg cgaaatcatg
    13201 ttgattccgt ggcaagacct ctcccccgaa acgctggaaa atttaattga aagctttgtg
    13261 ttacgtgaag gcaccgatta tggtgaacat gagcgtacac ttgaacagaa agtcgccgac
    13321 gtcaaacgcc agctacagtg cggagaagcg gtgctggtat ggtcggagct gcacgaaacg
    13381 gtcaatatca tgccgcgcag tcagtttcgc gaataacctg caaccgtcgg cggacgagat
    13441 aataatgata gctaagtcat ggagttacta tgtctgccaa acatccggtc attgcggtaa
    13501 caggatccag cggcgcgggg accaccacca ccagcctcgc gtttcgtaaa atattcgcgc
    13561 agttaaatct gcatgcagct gaggtggaag gcgacagttt tcaccgttac acccgcccgg
    13621 aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg gcatatcagc tacttcggcc
    13681 ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat tgaatacggt cagagcggca
    13741 aagggaaatc tcgcaaatat ctgcatacct acgacgaagc cgtaccctgg aatcaggtac
    13801 cggggacatt caccccctgg caacctttac cggaacccac tgatgtactg ttttatgaag
    13861 gtttacacgg cggcgtagtc acgccacagc ataacgttgc gcagcatgtg gacttactgg
    13921 tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa actgatccgc gacaccagcg
    13981 agcgcgggca ctcacgagaa gcagtgatgg actcagtagt gcgttcaatg gaagactata
    14041 tcaactacat cacaccgcag ttttcccgca cccatcttaa cttccagcgc gttcccaccg
    14101 tcgacacttc aaacccattc gcggcaaaag gtatcccgtc gctcgatgaa agctttgtgg
    14161 tgatccattt tcgtaatctg gaagggatcg atttcccctg gctgctggcg atgttgcaag
    14221 gctcattcat ttcccacatc aatacgttag tggtaccggg cggcaaaatg ggtctggcaa
    14281 tggaattaat tatgctgccg ctggtgcaac gattgatgga aggaaagaaa atcgagtaac
    14341 tctgctatta cgccggataa aatactatcc ggcttcacaa cgggatagtt aaatcacgcg
    14401 gcaaccactt catacgagtg agtaatattc accgcttttt ccagcatcaa cgccactgaa
    14461 caatatttct cagcagagag atcaaccgca cgcgcaaccg ctgcgtcttt caggtcgcga
    14521 ccggtgacga taaaatgcag attaatgtgc gtaaacaggc gtggtgcctc ttcgcggcgt
    14581 tcagaggtca attttacttc acaatcgacc acatcctgac gccctttttg caggatcgaa
    14641 accacatcga tggcactgca accacccgcc gccatcaaca ccatttccat cggacttggt
    14701 gctttatcgc ctgagttgcc gtccattaaa atctggtgac cagaggcgga ttcgcccaga
    14761 aaagttaacc cttcgaccca cttcactcgc gcttgcattt ttgctactcc actgcgtcaa
    14821 ttttcctgac agagtacgcg tactaaccaa atcgcgcaac ggaaggcgac ctgggtcatg
    14881 ctgaagcgag acaccaggag acacaaagcg aaagctatgc taaaacagtc aggatgctac
    14941 agtaatacat tgatgtactg catgtatgca aaggacgtca cattaccgtg cagtacagtt
    15001 gatagcccct tcccaggtag cgggaagcat atttcggcaa tccagagaca gcggcgttat
    15061 ctggctctgg agaaagctta taacagagga taaccgcgca tggtgcttgg caaaccgcaa
    15121 acagacccga ctctcgaatg gttcttgtct cattgccaca ttcataagta cccatccaag
    15181 agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt actacatcgt taaaggctct
    15241 gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga tcctctccta tctgaatcag
    15301 ggtgatttta ttggcgaact gggcctgttt gaagagggcc aggaacgtag cgcatgggta
    15361 cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca aaaaatttcg ccaattgatt
    15421 caggtaaacc cggacattct gatgcgtctg tctgcacaga tggcgcgtcg tctgcaagtc
    15481 acttcagaga aagtgggcaa cctggcgttc ctcgacgtga cgggccgcat tgcacagact
    15541 ctgctgaacc tggcaaaaca accagatgct atgactcacc cggacggtat gcaaatcaaa
    15601 attacccgtc aggaaatcgg tcagattgtc ggctgttctc gtgaaaccgt gggacgcatt
    15661 ctgaagatgc tggaagatca gaacctgatc tccgcacacg gtaaaaccat cgtcgtttac
    15721 ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta gcgcgccatt ttgtttcccc
    15781 cgatgtggcg cagactgatt tatcaccccg atatcaacta tgcacttcga caaacgctgg
    15841 tgctatgttt gcccgtggcc gttgggttaa tgcttggcga attacgattc ggtctgctct
    15901 tctctctcgt tcctgcctgt tgcaatattg cgggccttga tacgcctcat aaacgttttt
    15961 tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg tagcttactg acacagctgc
    16021 tactggcaaa agatgttccc ctgccctttt tgctgaccgg attaacgctg gtacttggcg
    16081 tcactgctga gttggggcca ttgcacgcaa aattgcttcc cgcatcgctg ctggcagcca
    16141 tttttaccct cagtctggcg ggatacatgc cggtctggga accgttgctc atctatgcgt
    16201 tgggcactct ctggtacgga ttgtttaact ggttttggtt ctggatctgg cgcgaacaac
    16261 cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc agattattgt gaagccaaat
    16321 acagcctgct tacccagcac accgaccctg aaaaagcgct gccgccgctg ctggtgcgcc
    16381 agcaaaaagc ggtcgatcta attacccagt gctatcagca aatgcatatg ctttccgcgc
    16441 aaaataatac tgactacaag cggatgctgc gtattttcca ggaggcgctg gatttacagg
    16501 aacatatttc ggtcagtttg catcagccgg aagaggtgca aaagctggtc gagcgtagcc
    16561 atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc cgctcgcctg cgcgtgctgg
    16621 ctgatgacat tctttaccat cgcctgccaa cgcgttttac gatggaaaag caaattggcg
    16681 cactggaaaa aatcgcccgc cagcatccgg ataatccggt tgggcaattc tgctactggc
    16741 atttcagccg catcgcccgc gtgctgcgca cccaaaaacc gctctatgcc cgtgacttac
    16801 tggccgataa acagcggcga atgccattac ttccggcgct gaaaagttat ctgtcactaa
    16861 agtctccggc gctacgcaat gccggacgac tcagtgtgat gttaagcgtt gccagcctga
    16921 tgggcaccgc gctgcatctg ccgaagtcgt actggatcct gatgacggta ttgctggtga
    16981 cacaaaatgg ctatggcgca acccgtctga ggattgtgaa tcgctccgtg ggaaccgtgg
    17041 tcgggttaat cattgcgggc gtggcgctgc actttaaaat tcccgaaggt tacaccctga
    17101 cgttgatgct gattaccacc ctcgccaact acctgatatt gcgcaaaaac tacggctggg
    17161 cgacggtcgg ttttactatt accgcagtgt ataccctgca actattgtgg ttgaacggcg
    17221 agcaatacat ccttccgcgt cttatcgata ccattattgg ttgtttaatt gctttcggcg
    17281 gtactgtctg gctgtggccg cagtggcaga gcgggttatt gcgtaaaaac gcccatgacg
    17341 ctttagaagc ctatcaggaa gcgattcgct tgattcttag cgaggatccg caacctacgc
    17401 cactggcctg gcagcgaatg cgggtaaatc aggcacataa cactctgtat aactcattga
    17461 atcaggcgat gcaggaaccg gcgtttaaca gccattatct ggcagatatg aaactgtggg
    17521 taacgcacag ccagtttatt gttgagcata ttaatgccat gaccacgctg gcgcgggaac
    17581 accgggcatt gccacctgaa ctggcacaag agtatttaca gtcttgtgaa atcgccattc
    17641 agcgttgtca gcagcgactg gagtatgacg aaccgggtag ttctggcgat gccaatatca
    17701 tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg tacgctggag cagcatttac
    17761 agcgggttat tggtcatctg aacaccatgc acaccatttc gtcgatggca tggcgtcagc
    17821 gaccgcatca cgggatttgg ctgagtcgca agttgcggga ttcgaaggcg taatgcaggc
    17881 taaaccatcg gataaggcat tcacgccgca tccgacatct tttgcctgat gcttcgtatt
    17941 tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt gcatcccttc atcgatatcc
    18001 gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat ccggtccggc attcagcacc
    18061 attacgccag cccctgcgcc cgcatacagg aaatcacgcg cccgaccttt gaactgtggc
    18121 ttaagctccg cgccaatcaa cagccccata ccgcgaatat cgctaaacac atcgtactgc
    18181 tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt tcgcctgaat gccttccagc
    18241 acttcagggg tattgatgat atcaaacgcc gcgcccgcta ctgcacagga cagaggatta
    18301 ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag aagcaatttc tgccgtggtc
    18361 agcatggcgc taatcgggaa gccgccgcct aacgctttcg cagaggtcag aatatccggc
    18421 gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc gccccatccc gcactgtact
    18481 tcatcaaaca ccaataatgc ctgatgttga tcgcacagct cgcgcaagcc ctgcaaaaac
    18541 tctggcgtcg ctgccgtcac accgccctcg ccctggatcg gctcaaccac caccgcacag
    18601 gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt taaaaggaac gtggatgatg
    18661 tctgccggtt ttggcccaaa gccgtcggaa tattttggct gcccgcccac cgagacggta
    18721 aacagcgagc gaccatgaaa agcgttatgg aaggcaataa ttttggtttt gaacggacta
    18781 tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg tttcgttagc ttccgtgccg
    18841 gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa tcagtttgcg cccaagacgc
    18901 agcgccggtt cattggtgaa aacgttactg atatgccaca gagtttcgcc ctgggttttt
    18961 aacgcgttca ccagcgcagg atggcaatgg cccaacgccg taactgcaat gccacccgcg
    19021 aaatcgacat actccttgcc ttgctgatcc cagattcggc tgccctgacc ttttaccgga
    19081 atgaactctg ccggagcata aatcggcagg atcacttcat cgaaagtcgc gcgtgtaatt
    19141 gctgtttgtt caattgccat ctcatgatca ccctgttacg cataaataaa tgtgaaatta
    19201 taatcacaaa atatgcataa aaaatcattg aatggcaatc agaaatcagc gatgcaggaa
    19261 attagccagc agttgatgtc cttgttcgct aagaatactt tctggatgga actgcacacc
    19321 ttccagatcc cactggcgat ggcgaatccc cataatctcg cgggtttcgc tccaggccgt
    19381 cacgtcaaag catgctggta atgagccaag ttccaccacc agcgaatggt agcgtgtcac
    19441 ggtaagtgga tttgccagcc cccgaaatac gccctcaccg ttatgtgtaa tcggcgaggt
    19501 tttgccgtgc atgacctttg cggcgcgcac aactttgccg ccaaatgcct gcgccattgc
    19561 ctgatgaccg aggcagacgc caagaatcgg caagcgcccg gcatagtggc gaataacgtc
    19621 aagggagatc ccggcttcat ctggcgtaca ggggccgggt gatatgacaa ttttttgtgg
    19681 tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg cgcttaacca gcacatccgc
    19741 ccccagttca caaaagtact ggtagaggtt ccaggtaaaa gaatcgtagt tatctataag
    19801 caggatcatg gcggctccgg tacgaaagaa ccgcgctatt ttactcagat tccccggctt
    19861 cgcttaccac tttgctaaat atcatctgca gcgcggtgag atcccccatt gccccactct
    19921 gatttgcctg attccaggct tctttttcga taccctgcca gctcagttta tatccggcat
    19981 gaatcgccag ttgctcgaag aagatccgct gtgccagtcc gcttcccaca cggaagggat
    20041 gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc gacgaacttc gctttctcca
    20101 ggccaaccag ataaccttct tcctccagat cctgcatcag ggcattgccc tctttttcga
    20161 tataagcaaa gtggcagaac ggcgtatcgc cctgataaat atcaacttca cgcagttgcc
    20221 ccgcccagtc gaaaatatcc tgatacagct ggcgatggat agtgcgcaaa tgcggtaaac
    20281 cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag cgccgtcatt tcgtaagcgg
    20341 cctgttccag ccgctgctgc tggtggatgt tcagccggtt acgcatgata tcaaggccag
    20401 gataaagata cggatcgcgc ccttcgccga atttatcgct catagtgcct cctcagctct
    20461 tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta aaggcatctc gacgccttca
    20521 agacggcgac tggcctggaa attacgatta cgctgaagct cccagagacg ggacttttgc
    20581 ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg tccatttgcc acaagtataa
    20641 gcggcaaatc cgggttacgc cgggagagc
//
LOCUS       DAZFVO020000089        20223 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFVO020000089 DAZFVO020000000
VERSION     DAZFVO020000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.089"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHKNB_RS14990"
                     /old_locus_tag="pgaptmp_002998"
     CDS             complement(138..884)
                     /gene="cutC"
                     /locus_tag="ACHKNB_RS14990"
                     /old_locus_tag="pgaptmp_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGU4196469.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHKNB_RS14995"
                     /old_locus_tag="pgaptmp_002999"
     CDS             complement(898..1464)
                     /gene="yecM"
                     /locus_tag="ACHKNB_RS14995"
                     /old_locus_tag="pgaptmp_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGU4196470.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1680..3413
                     /gene="argS"
                     /locus_tag="ACHKNB_RS15000"
                     /old_locus_tag="pgaptmp_003000"
     CDS             1680..3413
                     /gene="argS"
                     /locus_tag="ACHKNB_RS15000"
                     /old_locus_tag="pgaptmp_003000"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGU4196471.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPANELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3455..3499)
                     /gene="yecV"
                     /locus_tag="ACHKNB_RS15005"
                     /old_locus_tag="pgaptmp_003001"
     CDS             complement(3455..3499)
                     /gene="yecV"
                     /locus_tag="ACHKNB_RS15005"
                     /old_locus_tag="pgaptmp_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HGU4196472.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3590..4078
                     /gene="yecT"
                     /locus_tag="ACHKNB_RS15010"
                     /old_locus_tag="pgaptmp_003002"
     CDS             3590..4078
                     /gene="yecT"
                     /locus_tag="ACHKNB_RS15010"
                     /old_locus_tag="pgaptmp_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HGU4196473.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHKNB_RS15015"
                     /old_locus_tag="pgaptmp_003003"
     CDS             complement(4198..4590)
                     /gene="flhe"
                     /locus_tag="ACHKNB_RS15015"
                     /old_locus_tag="pgaptmp_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HGU4196474.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMMLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHKNB_RS15020"
                     /old_locus_tag="pgaptmp_003004"
     CDS             complement(4590..6668)
                     /gene="flhA"
                     /locus_tag="ACHKNB_RS15020"
                     /old_locus_tag="pgaptmp_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HGU4196475.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHKNB_RS15025"
                     /old_locus_tag="pgaptmp_003005"
     CDS             complement(6661..7809)
                     /gene="flhB"
                     /locus_tag="ACHKNB_RS15025"
                     /old_locus_tag="pgaptmp_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HGU4196476.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHKNB_RS15030"
                     /old_locus_tag="pgaptmp_003006"
     CDS             complement(8011..8655)
                     /gene="cheZ"
                     /locus_tag="ACHKNB_RS15030"
                     /old_locus_tag="pgaptmp_003006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HGU4196477.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHKNB_RS15035"
                     /old_locus_tag="pgaptmp_003007"
     CDS             complement(8666..9055)
                     /gene="cheY"
                     /locus_tag="ACHKNB_RS15035"
                     /old_locus_tag="pgaptmp_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HGU4196478.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHKNB_RS15040"
                     /old_locus_tag="pgaptmp_003008"
     CDS             complement(9070..10119)
                     /gene="cheB"
                     /locus_tag="ACHKNB_RS15040"
                     /old_locus_tag="pgaptmp_003008"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HGU4196479.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHKNB_RS15045"
                     /old_locus_tag="pgaptmp_003009"
     CDS             complement(10122..10982)
                     /gene="cheR"
                     /locus_tag="ACHKNB_RS15045"
                     /old_locus_tag="pgaptmp_003009"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HGU4196480.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHKNB_RS15050"
                     /old_locus_tag="pgaptmp_003010"
     CDS             complement(11001..12602)
                     /gene="tap"
                     /locus_tag="ACHKNB_RS15050"
                     /old_locus_tag="pgaptmp_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HGU4196481.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHKNB_RS15055"
                     /old_locus_tag="pgaptmp_003011"
     CDS             complement(12648..14309)
                     /gene="tar"
                     /locus_tag="ACHKNB_RS15055"
                     /old_locus_tag="pgaptmp_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HGU4196482.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14454..14957)
                     /gene="cheW"
                     /locus_tag="ACHKNB_RS15060"
                     /old_locus_tag="pgaptmp_003012"
     CDS             complement(14454..14957)
                     /gene="cheW"
                     /locus_tag="ACHKNB_RS15060"
                     /old_locus_tag="pgaptmp_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HGU4196483.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14978..16942)
                     /gene="cheA"
                     /locus_tag="ACHKNB_RS15065"
                     /old_locus_tag="pgaptmp_003013"
     CDS             complement(14978..16942)
                     /gene="cheA"
                     /locus_tag="ACHKNB_RS15065"
                     /old_locus_tag="pgaptmp_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HGU4196484.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELIITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16947..17859)
                     /gene="motB"
                     /locus_tag="ACHKNB_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /pseudo
     CDS             complement(16947..17859)
                     /gene="motB"
                     /locus_tag="ACHKNB_RS15070"
                     /old_locus_tag="pgaptmp_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
     gene            complement(17856..18743)
                     /gene="motA"
                     /locus_tag="ACHKNB_RS15075"
                     /old_locus_tag="pgaptmp_003015"
     CDS             complement(17856..18743)
                     /gene="motA"
                     /locus_tag="ACHKNB_RS15075"
                     /old_locus_tag="pgaptmp_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HGU4196485.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18870..19448)
                     /gene="flhC"
                     /locus_tag="ACHKNB_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /pseudo
     CDS             complement(18870..19448)
                     /gene="flhC"
                     /locus_tag="ACHKNB_RS15080"
                     /old_locus_tag="pgaptmp_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
     gene            complement(19451..19801)
                     /gene="flhD"
                     /locus_tag="ACHKNB_RS15085"
                     /old_locus_tag="pgaptmp_003017"
     CDS             complement(19451..19801)
                     /gene="flhD"
                     /locus_tag="ACHKNB_RS15085"
                     /old_locus_tag="pgaptmp_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HGU4196486.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4843 a         5565 c         4931 g         4884 t
ORIGIN      
        1 cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa
       61 aaaaaggccc ctgttgaaat tgcgggggcc tggtacgagc aagcatcata ttgggcgaca
      121 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
      181 accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg
      241 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
      301 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg
      361 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
      421 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
      481 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
      541 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg
      601 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
      661 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
      721 tgcacaggga tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag
      781 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
      841 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
      901 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
      961 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
     1021 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     1081 acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt cccaaccttc
     1141 gtgtgggtaa cgtttttccc cgggccacgg tagctccaca atagaaaact gccagtgcgc
     1201 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc
     1261 tgacaaaagc tcgccacact gttcaaaccc gcgacgccag cgttcagcag tggcgttttg
     1321 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggag
     1381 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     1441 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     1501 ccctgttgcc ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     1561 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc
     1621 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     1681 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
     1741 ctgcggattg cgaaccgcag gttcgtcagt cagcaaaagt tcagttcggc gactatcagg
     1801 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
     1861 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
     1921 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
     1981 cgtccgatcg tctcggtgtt tctacgccag aaaaacagac cattgtggtt gactactctg
     2041 cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg ctctaccatt attggtgacg
     2101 cagcagtgcg tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
     2161 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
     2221 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
     2281 acgaagatga agagttcgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
     2341 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaga
     2401 tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc gaaagcctct
     2461 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
     2521 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
     2581 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
     2641 cgaaatatcg ttatgaatca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
     2701 agcatcaaca cctaatgcag gcatgggcga tcgtccgtaa agcaggctat gtaccggaat
     2761 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
     2821 aaacccgcgc gggtggtaca gtgaaactgg ccgatctgct ggatgaagcc ctggaacgtg
     2881 cacgccgtct ggtggcagaa aagaacccgg atatgccagc taacgagctg gaaaaactgg
     2941 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
     3001 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
     3061 agtatgctta cacgcgtgta ttgtccgtgt tccgtaaggc agaaattaac gaagagcaac
     3121 tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
     3181 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtgatgtgtg
     3241 cttacctgta cgatctggct ggtctgttct ctggcttcta cgagcactgc ccgatcctca
     3301 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
     3361 cgctaaagct gggtctggat acgctgggta ttgagacagt agagcgtatg taatcgattt
     3421 ttcgtgagag taaagcctga tcagggttac ctgatcaggc ttttttattg ccatctaaat
     3481 gtattctgaa aatggacatg ccattgtttt ctgactgttg gataagaggc cagaagcgta
     3541 atatccggcc ccagggaaac gataacggtt gaatttaagg aataccgcag tgtttaaatt
     3601 tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata cggttatagt
     3661 aaacgaccat gacatttcag ccatcaaaga ttgttggcaa aaaaattcag atgatgatac
     3721 tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg atgcgcaatt
     3781 aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgtaaa
     3841 aaaacctgat accgaacaac ttaacttgct taaaaaatca cagcgagcct ggctggattt
     3901 tagggacaaa gaatgtgaat taatcctttc aaatgaggac gttcaggatt taagtgaccc
     3961 ttattctgaa tcagaatggc tctcatgtat gatcatacag accaatacgc gaactcgcca
     4021 gttgcagcta taccgtaact ctgaagattt ttatccaagc cctttgacaa gaggataatt
     4081 cacatctttt tggcatgttt tgttgcaagc tattcctgat aaataattgc aacaagacat
     4141 cgagcctttt tcactgagtt attaaacata ctcgcgagcg cgtaattttt ttgtccttca
     4201 gcgataattc acaatcactt cattacgttg tacctttagc ggtggaatta accgcccacc
     4261 gcctggcact tcccagataa atcgcaacgg ttctgctgcc gctataccgg aaaaggccac
     4321 ggtggttccg ctctgcccct ctaattcgac acaacgagat tgcgaacaca agcgaacccg
     4381 cagtcctgaa ggtgtcgggc cgataagctg ataacgccac gctaccagca tcatcaatcc
     4441 tgaagcaggt tgtcgcgtag aaagaggcgc agacgacatc gactcaccgc gatgatttag
     4501 cgtaattcct acactacttg cctgccacat cccctccccg gcggcttgca ccagcagcgg
     4561 aaacaataat attgctaata aggttctcat tatttgccgc caattgtcgc cgtcatgcgg
     4621 atatgtcggt tatcagacag ttccagattc gacaggacca ctaactgcgg caagctgcgg
     4681 cgcaggaagc gagacaataa tggtcgcagc gcgtggttca ccaacaatac tggcggcgca
     4741 cccagcatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg atccgccagc
     4801 cctggctcca gtcctccccc gccctgcagc gcctgtagta acaaacgttc cagcggtgta
     4861 tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt aatcgcccgt
     4921 cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cgctttggat gggcgcatgt
     4981 tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg gtactttttc atcgaggaga
     5041 ttttgcagca ctttatgcag tgtggtgagc gtgacgacgc caggaacgag atcttccgtc
     5101 agctttggca tctcctgggc gacgcgatcc aacagctgtt gcgcctcctg acgaccaaac
     5161 agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac cgtgctggcc
     5221 tcaaccactg tgtacccctg aatctgcgcc tgttctttta gcgcactttc aatccagata
     5281 gcattcaggc caaatgccgg atcgacggtc gcctcaccag gtaacgtccc ggcagcggtt
     5341 ccagggttaa tcgccagcca gcgccccgga taagcatcac cactgccaat ctccacgcct
     5401 ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat gtgcaccact
     5461 ggcggcagaa atcccatctc ctgggcaaat ttcttgcgga tactgcgtat acggcccaac
     5521 aactcaccat cctgctggaa atcgaccatc gggatcagtc gataacccac ttccattccc
     5581 agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggtatt attctctgcc
     5641 atttttaccg gtttgggttc gacaggcgct ttttgttcgc gtccgcgtat ccaccaggcc
     5701 agccccagca atccggcagt gaacagcaaa aataccaggt tcggcattcc aggcaccagg
     5761 ccgagtaaac cgagcacggc ggcgcttaac aacataacgc ttgggttact gaaaagctga
     5821 ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacgat gacccccgcg
     5881 gcggtagaaa tcaccagcgc cggaatttgt gccaccagac cgtcgccaat ggtcaatagt
     5941 gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc gaccagcaac
     6001 ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc gcgaacaaac
     6061 ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtcacttc ggagcggcgt
     6121 tttttcgcct catcttcacc aatcaatcca gcgttaaggt cggcgtcaat cgccatctgc
     6181 ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg cccggcacct
     6241 ttggtaatga ccataaagtt gatgatcacg agaatgacaa acactacgat accgatagcg
     6301 aaattgccac caacgaggaa gtgaccgaat gcttcgacca ccttcccagc cgccgccgcg
     6361 ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc cagacgcaac
     6421 aacgtagtaa acaacagaat ggtcggaaac gcagcaaact caagcgtgcg ctgggtaaac
     6481 atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa caggtcgagt
     6541 atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa aatcggtccg
     6601 gcaaggatct gccattgtgt cgatttcagg tttgcgggca ggcgcagcat cgcggccaga
     6661 ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg gaagatgagt
     6721 aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     6781 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     6841 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     6901 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     6961 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     7021 atccgctttc ggcacatcgg ccatcatccg acgccgtgca gcagctcgct gcatctgacg
     7081 gatccgccct ttaacatgag ggtcaccttc gctttgtttg aactcatcac gaatatcctg
     7141 ccgtgacata cgcagctttt tcaggtggct gaatatttgg aaaaagacgt caaatcccac
     7201 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     7261 acccatggcg gtaatcggag actcggccat caagcgcatc atctgcggcc agtgatgcca
     7321 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgctt taagcaactc
     7381 cgcgccagtc tgagccgaga acatccgttt aatgcccggt agcgggttga gtttggaaaa
     7441 cttcggctgc aaggatttgc cgctaaatac cagccctccc agcatgaccg gagaaatgag
     7501 cgccaccagc accacgccgc taatcagcgg cagcagcgcc agcatggctt ctctgatcag
     7561 cagaataatc tgcccgagga tcagattcgg gtcattgata atactgtgat caaaatgcag
     7621 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     7681 actaacgccc accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc
     7741 ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc
     7801 gtcagacacg tcgccaatcc tggaaagagt attaagcgtg aatgatgcca gagcgcaaag
     7861 cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg ctgccacaca
     7921 tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc gcctgatatg
     7981 acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca acaaatcgtc
     8041 cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag gtccattaag
     8101 caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt ccaacagcac
     8161 catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa tgacctgccc
     8221 ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt tagtaaagct
     8281 ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct gttaccagtt cacgggcgtc
     8341 ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg cttttgctga
     8401 tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg cccgctccgc
     8461 agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg ggatggcttc
     8521 cgccgcttcg gcaatggcct gatccagccc cagttcccgc aaactgtcgc gcagcatacg
     8581 cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag caggtttgat
     8641 tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt ttgttgagtt
     8701 tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc cccgcttgcg
     8761 ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact ggcaatgccg
     8821 acatcgcgcc atccgcacga attgttttca gcaattccag gccatccata ttgggcatgt
     8881 tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga gcgtcgacgc
     8941 catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta cgcactatgc
     9001 gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc gccatttcac
     9061 actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca tttgctggct
     9121 tacctggcta agatcgacca cttcgcagac accacccata ttgatggcct cgcgcggcat
     9181 gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgcccccg cctgacgcat
     9241 cgccaacatt cccgccgcgc cgtcgttgcc cataccggtc aggatcaccc caaccgcatt
     9301 acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc gatgacggtt
     9361 aaccgccggg ccatcgtgaa ttttgatttg gtaatttgcg ccactacgcg acagctccat
     9421 atgccgatcg cccggcgcga tataggcatg tcccggcaaa acacgttctc cgtcttcggc
     9481 ttctttaacc ccgatctgac aaagcttatt aagtctgtcg gcaaaagagc gggtgaaacc
     9541 aggcggcata tgctgggtaa ttaacagtgc cgggctggaa agcggcaacg gttgcagtac
     9601 gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt tttcagaact
     9661 caacaacggc cctgccttca gcgttgtcgg tgccgacaat ggcttatgtg ctgcaaggct
     9721 cgcctttgct gctgtacgca ccttttcagc aatcatttcg ctatacgcca gcattccttc
     9781 gcgaataccc agttgcggtt tggtgacaaa atctatcgcc cccagctcca gcgcgcgcag
     9841 cgtgacttct gaccctttgc cggtcaagga agaaaccatc acaacgggca ttggacgcaa
     9901 acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa catccagcgt
     9961 cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat caggcgcggt
    10021 cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct ggcgcatcag
    10081 tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt acttagcgca
    10141 tacaccgtct gaccacgcag cgtgaagcgg cgctcaaggt ggctaaagtt ttcagagtga
    10201 cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa aatctcctgc
    10261 tgggtatttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc aaacggcccc
    10321 ggcacggtgt actgtttcgc cagtagattc agcggggcaa aatcaacata gttcgccagc
    10381 tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa ataccgttgc
    10441 agttgctgcg gcgtcaggtt tttcaactct tcatggcgat aaataccgct tctggctttt
    10501 tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg cgcggtgccc
    10561 aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga agccgccgcg
    10621 ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag agggaaatgg
    10681 tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa cgcctgccac
    10741 tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc cgtcagtccc
    10801 agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg gtcagccaga
    10861 acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc gtcggaaagc
    10921 gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag agatgaagtc
    10981 atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg cgccgactca
    11041 tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga aagatggtcg
    11101 gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga ggcgttctgc
    11161 tgagtcacct gatccatctg gctgacagcc tgtgcaacct gctcaatccc ccgctgttgt
    11221 tcttccgacg cagaggcaat ttctcccata atgtcgttca cgcgggtcac cgaactgaca
    11281 atatcgatca tggtcgcggc ggcgttattc accagtttcg aaccctgctg gacacgattg
    11341 actgactctt cgatcagccc tttgatctct tttgctgcct gcgcgctacg gctggcaaga
    11401 ttgcgcactt cacctgccac taccgcaaaa ccacgcccct gctctccggc gcgagccgct
    11461 tccactgccg cattcagggc cagaatattg gtctggaaag caattccgtc gataacgctg
    11521 ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg agtcatggta
    11581 ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgccag ttccgacgcc
    11641 tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat actggcggcc
    11701 gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt attgcctgcg
    11761 acaatctccg cgataccaat gtgcatctcc tggcttccct tacgcacatc acttaccgtc
    11821 ccacgcaaag cctgctgcat ggtcttcaga ctggcaaaaa tggcggtgat ctcattacga
    11881 ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa atggctaccg
    11941 ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact gctgatgtag
    12001 atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata gttacgctgg
    12061 ctttgcgcac tggcggcttc cagcacatgg ttgatctcca gctgccaggc ctcaaagtta
    12121 acgtcaaacg cattctgtga ttcctgcacc ggcgcagtga ggaaatccga aagttgatta
    12181 ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa acttttctcc
    12241 gtctcttttt gcaacgccct gacgtgctcg ttgcccgcag tcatcgccat aaaactttta
    12301 aacagcgtgg tggattgcgt cagactggcg cgcgccgtcg tcatcaacgt tttaatgtca
    12361 tccgccggat agctaagcgc cgtcagagtg cccgctttgt tcagcgcggt actggcctgt
    12421 aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg ctcgacctga
    12481 ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact gccaatctgc
    12541 aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat acgattaaac
    12601 atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca aaatgtttcc
    12661 cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg ctcactggca
    12721 ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg gaacgcggaa
    12781 accgcttgcg ttaaacgact cgcctgttct tccagcgcag cggcggcggc agctgattcc
    12841 tgcaccagcg atgcgttctg ttgcgtgacg cgatccattt ccgaaaccgc caatgcgact
    12901 tgatcgatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat aatgtcagtc
    12961 acgcgagtga cagcattgac gatattgttc attgtttccc cggcgctttc gaccagcacc
    13021 gaaccggtat caacgcgtga gacggagtct tcaatgaggg ctttgatctc ttttgccgcc
    13081 tgggcgctac gactggcaag attacgcact tcacccgcca ccacggcaaa accacggccc
    13141 tgttcacccg cacgcgcggc ttcaaccgcg gcattcagcg cgaggatatt agtctggaag
    13201 gcaataccgt cgataacgct gataatgtcg gcaattttct tcgaactatc ggcgatctca
    13261 tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt gtcggaggca
    13321 ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac tgtcgcggtg
    13381 agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc agtacgggag
    13441 gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc atctgaacct
    13501 tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac gctctgcgcc
    13561 aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt accaccggcg
    13621 atttcgcgaa tgtgagcaat aatttttgcc agcggagtaa gcaacatacg gcgaatgccg
    13681 taccacgcca ccagcagaat caataccacc accagcgcga taaccgccag ttgccactgg
    13741 gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt ttcactgctg
    13801 agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt tggctgagcg
    13861 aaataagctc cagtattgcc ataatctaga taatcaatca gttcagttaa cgctgtgtaa
    13921 tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg taacggtgcc
    13981 atgcttttga attttttata atgcgtcgct gcctgcgcca atgttttcct ggcgctatcg
    14041 agcaattcaa ctttggcgtt actttgttga ttggaggaat ccatcatcat ccgtaccgct
    14101 gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt cagctcgccc
    14161 tgctgttccc gtaattgatt ggaaaccaca aagctcttct ggctatggtg aagggaagaa
    14221 aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac cattaccaac
    14281 agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa gttgatctcg
    14341 ttatcggcaa ggagggggga aactttattg ctgatgccac ccgccgcgaa attgaaataa
    14401 aaaacccgat gcgcagatca tcgggttcat ttcaattgag gaaatcggga gaattacgcc
    14461 acttctgacg ccgcgctatc taacagcgcc atctcttcgc tgttcagcag tttttcgatg
    14521 ttcaccagaa tcaacatccg gtcgcccagt gcgcccagtc cagtgagata ttctgttgaa
    14581 agcgtcacgg caaattccgg tgccggacga atttgctccg ccgtcaatga aagcacgtct
    14641 gagacgccgt caaccacgat gccgaccacc cgctgtccga gattcaggac gataactacc
    14701 gtgttgtcgt tatagtccac atccacctgg ctgaacttaa ttcgtaagtc aacaatcggc
    14761 acaataaccc cgcgcagatt cgtgacgcct ttgataaacg ctggcgtgtt cgcaatccgt
    14821 gttacctgat cgtagccacg gatctcctgc actttcagga tatcaatacc gtactcttca
    14881 tcaccaaggg taaataccag aaattcctgg cctgacggct cgctggccag ctttgttaca
    14941 ttcgtcatac cggtcatatt gttacctttt tactcattca ggcggcggtg ttcgccatac
    15001 gttgttcgcg gtttatcgcc tgcaaggcgg agacatcaac aatcagtgcc acgctgccgt
    15061 cgccaagaat ggtcgcagca gaaatgccgg ggactttgcg atagttactt tcaaggtttt
    15121 taaccacaac ctggtgctga ccaattaatt gatccaccag caaggcgtag cggcgaccgc
    15181 cactttgtaa gatcaccaca attccctggg tggcttcggt tttcgcgccc gcgacgttga
    15241 acactttcca cagttcgacg atgggcagat attcaccccg cacttccagc acccgctcgc
    15301 cgccggccag tggatgcaga tcggcttcac ggggttgcag tgattccata acagcattca
    15361 gcggcagaat gaaaacttca tccgcaacgc gtacggacat gccgtcgagg atggccagcg
    15421 tcagcggcag taaaatgcgg atcgtagtgc cagtaccctg cttcgactgg atttcgacat
    15481 gaccgcccat ctcctggata ttacgtttaa cgacgtccat gccgacgccg cgcccggaga
    15541 cgtcggtgac ctgctctgcc gtggagaagc ctggtgcaaa tatcagcatc gcgacttcgt
    15601 cgtcgctcat gttttcgctg acagtcaaac cttgcgaggc cgcttttgcc agaattcgct
    15661 cacggtttag ccccgcccca tcgtcggtca cttcaatgca aatgttgccg ccctgatgtt
    15721 cggcagacag aattaaattt ccgacgctgt ttttacctgc ggcgagccgt ttttctggca
    15781 gttcaatacc gtgatcgagg ctattgcgta ccaggtgggt cagcgggtcg ataatgcgtt
    15841 ctatcaggct cttgtcgagc tcggtggaac tgcccaccag cgtcagttct acctgcttgc
    15901 cgagttttcc cgccagatca cgcaccagcc ggggatagcg actaaagaca tattccatcg
    15961 gcatcatgcg aatcgacatc actgattcct gcaaatcacg ggcgttacgt tgtaactgcc
    16021 ccatgctggt aatcaaatca ccatgattaa ccgggtccag ttcgctggaa cgctgggcaa
    16081 gcatggactg ggtgataatc agctcgccga cgaggttaat taattgatca accttttcta
    16141 ccgctacacg gatgctggtg gattcactgc tgcgcgtcgt tttttcccgc tccacgcgac
    16201 cggttggcgc ttgttcggct gccagtttaa gcactggtgg ggtggatatt tttggcgaga
    16261 cttctactgt ttcaaaggta atctgatcgg cttcaatcac aaaacagagt accgctgtga
    16321 tgtcatcttc ggcgatatcg cccggtaata ttgccgagag agaatccgcc cctttcacca
    16381 cgtcagttaa cgttgtcaga tgccccagct cttcttccag caggtcgact tcgccggcct
    16441 tcaggcgcga aaggataatt cgtcgcggcg actgactgcg actctgctca tcttgcggtt
    16501 cacttttggc aaccacactt aatcgggtca ctgcggatgg cgtttcgcct ttcgcttcta
    16561 atgccagttg acgcaaggcc tggcagatat aatcgaagct ggcggcatcc ggctcttgcg
    16621 actgtttata agcgtcgagc tgttcttgca tgatgtcctt cgtttccaaa aacagattga
    16681 taatgtcggt gttgagttgc atctcacctc gtctggcttc atcgagcagg ttttccatca
    16741 gatgcgtggt ttcctgcaaa acgctgaagc caaaagttcc tgcccctcct ttgatcgagt
    16801 gggcagcccg aaagatggca ttcaattgtt cggcatctgg cgcttccggc tgcaaaacca
    16861 gcagatgctg ctccatatca gccaacagtt cgtccgcttc atcaaaaaat gtctgataaa
    16921 aatcgcttat atccatgctc acgctgtcac ctcggttcgg ctgatggcat tgtgggaaca
    16981 ctgacctgtg gtgcaacctc aggtttttcc agggcgctta ctggctcatt ctggctttcg
    17041 gcgttttcat gcaaaatggc ctgttcggct tgtttgttca gtaccagcag gctgatgcga
    17101 cggttgacgg catcatcagg tccgcgatcg cttaagcgca tcgttgccgc catgccgacg
    17161 acacgtaaca ctttgccgct atccaaccct ccgaccatca gttcgcggcg ggatgcattg
    17221 gcccgatcgg cagaaagctc ccagttgcta tatcctttct caccgctggc gtaggggaaa
    17281 tcatcggtat gacctgaaag gctaatacgg ttgggaatac cgttcagtac aggcgcaatg
    17341 gcacgcagaa tgtcgcgcat atagggttcg acatcggcac tgccggttct aaacatcggg
    17401 cgattctggc tatcgatgat ctgaatacgt agaccttcct ggaccagatc gattttgaga
    17461 tggggacgta acgcccgcag tttcggatcg gacgagatca ccccgcaatt tccgcaatcg
    17521 actttgctcc atgcgttttt tcagctcttc gatgttcggc tgcttattca cttccccctg
    17581 gctttgggtg taatcatcac caccgccggg aattgggctt tcactattag aaatgcgatc
    17641 gccgcccgta accgcagtcg ccagtggagt ccggaagtac tccgcaatct gaatcagctc
    17701 ttttgggctg gagatggaga tcagccacat caccagaaaa aaggccatca tcgcagtcat
    17761 aaagtcggca taagcaatct tccacgatcc atgtgctgcc ccgtggcttt tggctttgcg
    17821 tcgtttgacg acaataatcg gatgcgcttg attcttcatg cttcctcggt tgtcgtctgt
    17881 tgttgcggat ttttcaccgc acgcacatgc tcttccagtt caataaacga cggacgttcg
    17941 ctggaataga gcgttttgcg accaaactca acggcgatag gcggtgcgta accgttcaga
    18001 ttagaaagca gagtgacttt gacgcactgc atcattttgc tggtttcggc gcttttctga
    18061 cgtaaaacac tcgctaatgg ggaaataaat ccgtaagcca ataaaatgcc gaggaaagtc
    18121 cccaccatcg catgtgcgat aagcgcaccc agctcggcgg caggacgatc ggctgaacct
    18181 aacgcgtgaa cgacccccat tacagccgcg acaataccaa acgccggaag tgagtccccg
    18241 accagcgcca gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc aatctcttca
    18301 tccatcaggg cttcgatttc gaaggtgttc atatgaccgc tgataatcag acgcagataa
    18361 tcgacgataa aatcaagcat gacgctatcc gcgaggatgc gtgggtagct ggcgaagatc
    18421 tcgctctcac ggggattttc aatatcacgt tccagcgaaa acatccccat ctgccgcgat
    18481 ttcgccatca accgataaag cagagccagc agatccatat acattgcttt ggtgtatttg
    18541 gagcgacgaa acagcaacgg cagcgccttc agcgtgcctt taatcgcttt gccattattg
    18601 ccgacgataa acgacccaat ccctgcaccg gcaataatca ccagttcagc gggttgatag
    18661 agtgctccaa ggcttccacc ggtcatcaaa taaccgccga aaactgtacc gagaacaacc
    18721 aggtaaccta ataagataag cacgacatca tccttccact gttgaccatg acaggatgtt
    18781 cagtcgtcag gcgttaacgc gcgattgggg caaaaaaaag cagcggtacg tcgttaccgc
    18841 tgctggaatg ttgcgcctca ccgtatcagt taaacagcct gtactctctg ttcatccagc
    18901 agttgtggga taatatcggc aggattctgg gaaagtttac gtctttttac tgcccgggat
    18961 ggcggttgac attagctgca ggcaaagctg ccaacaggct ggtgagcgtg ggtaataaaa
    19021 ttgccgccgc agcagttgca gctggaaagt tgcagtaatc cactttcaac aaaccgcacc
    19081 aatgtccagg cacgggttaa tgccagcagt ggtccttctt ctgcttgtgg gcactgttca
    19141 aggtataaac ggtaggcttt gatcaccgca tcgacgccat tacacaaacc ggttttcagt
    19201 aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt tttgttccca ggtcataaac
    19261 cagtcggttg agaatggcag catgcctttc ggcggtgggc ttccgcgcag ttctttataa
    19321 agttttatca ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg caaacgagct
    19381 cccagggtga tcaattccat tgccagctga atatcccgcg cttcctgaac aatgcttttt
    19441 tcactcatga tcaggccctt ttcttgcgca gcgcttcttc aggctgatta acatcattca
    19501 gcaagcgtgt tgagagcatg atgccggtat gaatttgctg gagatcgtca acgcgggaat
    19561 cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa accagttgat
    19621 tggtttctgc cagcttaacc atttgcggaa gagtcagtgc cgctaacgtt gtcgccattt
    19681 cttcatttat gccgagacga aacatagcgg acgctttgtc ctgaacaatc aaacgctgtg
    19741 caagtagtaa atatgacaag ttgatgtcat aaatgtgttt cagcaactcg gaggtatgca
    19801 ttattcccac ccagaataac caactttatt tttatgcggt ttcaccgcac cccgtgatgt
    19861 cgccgggaag ccccggtaaa aaataattag cattagaata gttgcgataa gctgcaataa
    19921 gcagaaccac ctttttggtt taatatgtcc ttacaaatag aaatgggtct ttacacttat
    19981 ctaagatttt tcctaaatcg acgcaactgt actcgtcact acacgcacat acaacggagg
    20041 ggggctgcga ttttcaataa tgtgtgatgc agatcacaca aaacactcaa ttacttaaca
    20101 taaatgtata agtcatactt ttgttttggg tgtatttcaa tctgttaaaa agtttttcgc
    20161 tacgctagca agcaaaaatg aaacaggaat aatcgaaatg ggatgttgcg cacagttaaa
    20221 ata
//
LOCUS       DAZFVO020000090        20210 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFVO020000090 DAZFVO020000000
VERSION     DAZFVO020000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.090"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..882
                     /gene="yieE"
                     /locus_tag="ACHKNB_RS15090"
                     /old_locus_tag="pgaptmp_003018"
     CDS             1..882
                     /gene="yieE"
                     /locus_tag="ACHKNB_RS15090"
                     /old_locus_tag="pgaptmp_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HGU4196487.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1087..2556
                     /gene="lysP"
                     /locus_tag="ACHKNB_RS15095"
                     /old_locus_tag="pgaptmp_003019"
     CDS             1087..2556
                     /gene="lysP"
                     /locus_tag="ACHKNB_RS15095"
                     /old_locus_tag="pgaptmp_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HGU4196488.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPNTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2849..4840
                     /gene="cirA"
                     /locus_tag="ACHKNB_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /pseudo
     CDS             2849..4840
                     /gene="cirA"
                     /locus_tag="ACHKNB_RS15100"
                     /old_locus_tag="pgaptmp_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="ACHKNB_RS15105"
                     /old_locus_tag="pgaptmp_003021"
     CDS             complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="ACHKNB_RS15105"
                     /old_locus_tag="pgaptmp_003021"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HGU4196489.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5966..6634
                     /gene="folE"
                     /locus_tag="ACHKNB_RS15110"
                     /old_locus_tag="pgaptmp_003022"
     CDS             5966..6634
                     /gene="folE"
                     /locus_tag="ACHKNB_RS15110"
                     /old_locus_tag="pgaptmp_003022"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGU4196490.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6651..7808
                     /gene="yeiB"
                     /locus_tag="ACHKNB_RS15115"
                     /old_locus_tag="pgaptmp_003023"
     CDS             6651..7808
                     /gene="yeiB"
                     /locus_tag="ACHKNB_RS15115"
                     /old_locus_tag="pgaptmp_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HGU4196491.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7950..8990
                     /gene="galS"
                     /locus_tag="ACHKNB_RS15120"
                     /old_locus_tag="pgaptmp_003024"
     CDS             7950..8990
                     /gene="galS"
                     /locus_tag="ACHKNB_RS15120"
                     /old_locus_tag="pgaptmp_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HGU4196492.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9270..10268
                     /gene="mglB"
                     /locus_tag="ACHKNB_RS15125"
                     /old_locus_tag="pgaptmp_003025"
     CDS             9270..10268
                     /gene="mglB"
                     /locus_tag="ACHKNB_RS15125"
                     /old_locus_tag="pgaptmp_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HGU4196493.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10329..11849
                     /gene="mglA"
                     /locus_tag="ACHKNB_RS15130"
                     /old_locus_tag="pgaptmp_003026"
     CDS             10329..11849
                     /gene="mglA"
                     /locus_tag="ACHKNB_RS15130"
                     /old_locus_tag="pgaptmp_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HGU4196494.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11865..12875
                     /gene="mglC"
                     /locus_tag="ACHKNB_RS15135"
                     /old_locus_tag="pgaptmp_003027"
     CDS             11865..12875
                     /gene="mglC"
                     /locus_tag="ACHKNB_RS15135"
                     /old_locus_tag="pgaptmp_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HGU4196495.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12946..14181)
                     /gene="preA"
                     /locus_tag="ACHKNB_RS15140"
                     /old_locus_tag="pgaptmp_003028"
     CDS             complement(12946..14181)
                     /gene="preA"
                     /locus_tag="ACHKNB_RS15140"
                     /old_locus_tag="pgaptmp_003028"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HGU4196496.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14175..15413)
                     /gene="preT"
                     /locus_tag="ACHKNB_RS15145"
                     /old_locus_tag="pgaptmp_003029"
     CDS             complement(14175..15413)
                     /gene="preT"
                     /locus_tag="ACHKNB_RS15145"
                     /old_locus_tag="pgaptmp_003029"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HGU4196497.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15732..15971)
                     /locus_tag="ACHKNB_RS15150"
                     /old_locus_tag="pgaptmp_003030"
     CDS             complement(15732..15971)
                     /locus_tag="ACHKNB_RS15150"
                     /old_locus_tag="pgaptmp_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HGU4196498.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15974..16693)
                     /gene="sanA"
                     /locus_tag="ACHKNB_RS15155"
                     /old_locus_tag="pgaptmp_003031"
     CDS             complement(15974..16693)
                     /gene="sanA"
                     /locus_tag="ACHKNB_RS15155"
                     /old_locus_tag="pgaptmp_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HGU4196499.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16843..17727)
                     /gene="cdd"
                     /locus_tag="ACHKNB_RS15160"
                     /old_locus_tag="pgaptmp_003032"
     CDS             complement(16843..17727)
                     /gene="cdd"
                     /locus_tag="ACHKNB_RS15160"
                     /old_locus_tag="pgaptmp_003032"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGU4196500.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17857..18552)
                     /locus_tag="ACHKNB_RS15165"
                     /old_locus_tag="pgaptmp_003033"
     CDS             complement(17857..18552)
                     /locus_tag="ACHKNB_RS15165"
                     /old_locus_tag="pgaptmp_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HGU4196501.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18549..18947)
                     /locus_tag="ACHKNB_RS15170"
                     /old_locus_tag="pgaptmp_003034"
     CDS             complement(18549..18947)
                     /locus_tag="ACHKNB_RS15170"
                     /old_locus_tag="pgaptmp_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HGU4196502.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19186..20133
                     /gene="dusC"
                     /locus_tag="ACHKNB_RS15175"
                     /old_locus_tag="pgaptmp_003035"
     CDS             19186..20133
                     /gene="dusC"
                     /locus_tag="ACHKNB_RS15175"
                     /old_locus_tag="pgaptmp_003035"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HGU4196503.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4938 a         5001 c         5273 g         4998 t
ORIGIN      
        1 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
       61 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgaccgac
      121 ctggaagggc agcttggcgt gcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
      181 gaacacgggc ggctgctcta tccgcgtgcg ttggcattgc ttgaacaggc ggttgaaatc
      241 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
      301 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
      361 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
      421 attggtttta ttgaaggacc gtgccacagc actgaaatta tttctgaacc gtggctggaa
      481 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg cccgtggtcc ggtcacctta
      541 gagcagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
      601 gtcgattatc tattgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
      661 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgg
      721 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
      781 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
      841 cgctttctgg actattgcga tcccgcgaat gtaccgcgtt aagttgctgt acaagaacat
      901 gctggtgctg tgtcgatttc gtgacgcagc gccctcagca tgcattcgcc agaaaagaga
      961 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
     1021 tgccggaagg attgccaatc gtctgctaca atcgcgcctc atttttaaga tggatagcat
     1081 ttttgtatgg tttccgaaac taaaaccaca gaagcgccgg gcttacgccg tgaattaaag
     1141 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
     1201 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
     1261 ggcctgatgg tttacttcct gatgaccagt ctcggtgaac tggctgcata tatgccggtt
     1321 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
     1381 ggctggaact actggtacaa ctgggcggtg actatcgccg ttgacctggt tgcagctcag
     1441 ctggtcatga gctggtggtt cccgaataca ccgggctgga tctggagtgc gttgttcctc
     1501 ggcgttatct tcctgctgaa ctacatctca gttcgtggct ttggtgaagc ggaatactgg
     1561 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
     1621 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
     1681 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
     1741 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
     1801 ccacgcgcgg tacgtcaggt gttctggcga atcctgttgt tctatgtgtt cgcgatcctg
     1861 attatcagcc tgattattcc gtacaccgat ccgagcctgc tgcgtaacga tgttaaagac
     1921 atcagcgtta gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
     1981 gtgatgaacg cagttattct gacggcggtg ctgtcagcgg gtaactccgg tatgtatgcg
     2041 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
     2101 ctgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtctg
     2161 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
     2221 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
     2281 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
     2341 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
     2401 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
     2461 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgttac
     2521 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
     2581 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
     2641 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgccgca atcaaaaaag
     2701 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
     2761 accgcgatgc tgccgtacgc aaggggacgt gaagaagatg tgagcgataa cccattttat
     2821 tttcgtagtt acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg
     2881 gctgtgtttg tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga
     2941 aacgatggtt gtcactgcat cttccgttga acaaaacctc aaagatgcac ccgccagtat
     3001 cagcgtcatt acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct
     3061 caaagaagtg cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat
     3121 tcgtggtctg gacagcagct acaccctgat tctcgtcgac ggtaaacgcg ttaactcccg
     3181 caatgccgtc ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga
     3241 acgtattgaa gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg
     3301 tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga
     3361 taccaccatt caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag
     3421 tggaccatta attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga
     3481 aaaggatgac ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg
     3541 attctccagc cgcgacggca atgtcgaatt tgcctagaca ccgaatcaaa atcacgattt
     3601 tactgccgga tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg
     3661 cctggaacgc cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga
     3721 actgaaatac tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac
     3781 ttccgaaagc aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt
     3841 tctcacggtt ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg
     3901 gggaaccagc tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg
     3961 gatcttcgag ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg
     4021 tgaacactgg agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa
     4081 agggggctgg gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac
     4141 gagcaattcc tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac
     4201 cagcgaaagt tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggt
     4261 tgaatccagc gttaccgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac
     4321 gtctgacgtc aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa
     4381 cggacggcgc ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt
     4441 ggaaaccgaa ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta
     4501 caacgatggt cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca
     4561 tactgctaac ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt
     4621 ttctggtcac tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg
     4681 ttataccatc tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc
     4741 aggcgtgctg aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga
     4801 cggacgtcgt tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac
     4861 cgggcggact ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa
     4921 gaggcgatga agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac
     4981 gctttctggc gcgcggcttc tgccagtacc gcaggttgca actggtcggc aagaaactga
     5041 tcattatcgc cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc
     5101 agcgcgcaac tgtcccattc cagccatgca tttttgtcct cacctaaata gctgctaaac
     5161 gctttgattc cccacgggac gctgcacgga ttcacaattg gcgcaaaggc cgaaacgctg
     5221 gtgtatttac ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga
     5281 ccgctaatgg cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca
     5341 tcgcgcagat aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga
     5401 taaaagcctg cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc
     5461 gggctggtgt ctggcatcac cagtacaatc cccagttctg ccgccacgcg ctgggcaccc
     5521 gccttggtgg tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt
     5581 ggcggggtgt gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag
     5641 gtactggagt cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc
     5701 atttccatgc gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca
     5761 atgtgctgtg agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat
     5821 gaaaggcagc ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc
     5881 acgcacaata atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc
     5941 gtcacacctg caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca
     6001 tgaagcgtta gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga
     6061 taacgaaacg cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa
     6121 tctcgacctg gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt
     6181 cgatgaaatt ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa
     6241 caaaatgaag gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga
     6301 acaccatttt gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt
     6361 gatcggtctg tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca
     6421 ggaacgtctg acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt
     6481 ggctgtctcg atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac
     6541 cagtgccaca acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca
     6601 cgagtttctg cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca
     6661 acgtcacgct cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca
     6721 gcgcctttgg gctaccaaag gcggcttatc tcaatcccgc ctggtacggt gctattacgc
     6781 cgcaggatgc atggacctgg gcatttcttg atctcatcgg ccaggtgaaa ttcctcacgc
     6841 tttttgcgct gctgtttggt gctggactgc aaatgttgct gccccgtggc agacgctgga
     6901 tccagtcgcg gttaacgctg ttagtcttat tgggctttat tcacggttta ttgttctggg
     6961 acggcgatat tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg
     7021 atgcgccatc ggtaaaaagc ctgtttaata ccggcgtcat gctttatctg gtggggcttg
     7081 gcgttttgct gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg
     7141 atgcatcggc tattttgtat gaaaaatact ggaagcttca cggcggcgtt gatgcgatca
     7201 gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc
     7261 aactggctgg gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc
     7321 agttcagctt acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca
     7381 ttaaccttcc tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct
     7441 tcttacttca aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc
     7501 tgttttatgg cttctggccg caattaagcc gctttaagct ggtgctggca atcgcctgcg
     7561 tcggacggat ggcgctgacc aactatctat tgcaaacgct gatttgtacc acgctttttt
     7621 accacctcgg tttgtttatg cattttgatc gcctggagct gctggcgttt gttattccgg
     7681 tatggctggc gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg
     7741 tggaatggct ctggcgtcag ttaactttgc gtgctgccgg aacggcaata tctaaaacat
     7801 caagataacg atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt
     7861 gactcgattc acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat
     7921 ttactgcaat ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg
     7981 ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg
     8041 acacgcgtga agcagtaatg aaagccgtga gtgagctgga ttatcggcca aatgccaatg
     8101 cccaggcgct ggcaactcag gttagcgata ccattggcgt ggtggtgatg gacgtttctg
     8161 atgctttttt cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat
     8221 acgtgctaat cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt
     8281 taattcgcca gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac
     8341 tggcgcaatt tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt
     8401 acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt
     8461 tgctgaataa cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg
     8521 acgccatgcg taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg
     8581 aaagctggat tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac
     8641 tgctggggcg caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg
     8701 gcgcactgac agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg
     8761 gtttcgatga tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc
     8821 ccattgcttc aatgactaaa ttagccaccg aactggcctt gcagggggca gcaggcaata
     8881 ttgatcctcg tgccagccac tgttttatgc cgacgttagt gcgtcgtcat tctgtcgcaa
     8941 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
     9001 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg
     9061 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
     9121 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
     9181 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
     9241 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
     9301 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
     9361 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaagctatt gagcaagatg
     9421 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
     9481 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
     9541 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
     9601 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
     9661 ttggcactga ttccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
     9721 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcagttcgta ctactgaaag
     9781 gtgagccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gagctgaacg
     9841 ataaaggcat caaaactgaa cagttacagt tagataccgc aatgtgggac accgctcagg
     9901 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
     9961 ttgctaacaa cgatgcgatg gcaatgggtg cggttgaagc gctgaaagca cacaacaagt
    10021 ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc tctggcgctg gtgaaatccg
    10081 gtgcactggc gggcaccgta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    10141 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    10201 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca
    10261 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    10321 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    10381 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    10441 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    10501 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    10561 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    10621 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    10681 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    10741 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    10801 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    10861 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    10921 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    10981 aagttaccgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    11041 tggacaagat catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    11101 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    11161 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    11221 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    11281 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    11341 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    11401 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    11461 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    11521 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    11581 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    11641 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    11701 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    11761 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    11821 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    11881 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    11941 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    12001 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    12061 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    12121 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    12181 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    12241 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    12301 atggaatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    12361 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    12421 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    12481 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    12541 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    12601 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    12661 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    12721 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    12781 tcggcgtaaa cccatactgg cagtacatca tcaaaggggc gattattatc ttcgccgtag
    12841 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgtggatat tgccggatgc
    12901 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    12961 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    13021 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcgggt
    13081 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    13141 acagccaaca catttatcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    13201 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcagcg aatcaaatcc
    13261 ctgatcggcg agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta
    13321 ctgcatgatg ccggtggtca cctgtaacgt tgctgcgccg agcaataaaa actcagccgc
    13381 atcctcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    13441 ggtgcgcatc tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    13501 ggaaatactc gattttccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    13561 ggtgatggat ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc
    13621 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    13681 ggaaccccgt ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc
    13741 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    13801 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    13861 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc tgacgcaggg cggccagatt
    13921 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    13981 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    14041 tttaaacacc acgccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    14101 gcctacaggc gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat
    14161 cgaaagatct ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg
    14221 gcttctttcc cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca
    14281 gcagcaaaga cttgcggatc gcgggtctga taattttgtg ttttgatggt gttacgctgc
    14341 ggctctaact ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta
    14401 tctgccgcca tcgtcagttc gcccggtaac cgtacatgct taaacgtgac tttattgcct
    14461 tccacggcta ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca
    14521 aactcttttt cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc
    14581 tgacagccga gaactttcag cgtgctggct acgtccatcg caacatcacc gccgccgata
    14641 attaatgcgc tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaggaag
    14701 tcgacggcaa tctcaacgtc gctatgctca aacagcgata acccggaacc gctcgataac
    14761 cccacggtga ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta
    14821 ccgacttcgt tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca
    14881 tccagcactg attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg
    14941 tgcgcttctt tctcataaat cgtgacgtca taaccctggt ttgtcagtgt tacactggcc
    15001 tgcaatccgg caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc
    15061 tgataaattt ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcgaccg
    15121 atatcaatgg gagtatcaac accggcacgg gtacaaccgc tttgacataa tttctccgtc
    15181 gggcacactc tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg
    15241 cctttaaaat tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg
    15301 caagcctgac tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc
    15361 agtaaaggcg taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct
    15421 tccttaatgc tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc
    15481 atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc ctggggactc
    15541 actcacttgt cgccttcctg caactcgaat tatttagagt ataaatattg cgtgctgata
    15601 ttaaggctat gcgcgtgggg aaatttatac tgtgaaagga ttcacaaata gctgcggttc
    15661 agaatgttat atccgcagct tattgagtga agtaataatt attctcaata attaacagaa
    15721 aagagaaaat attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg
    15781 aagccgatat atgccaccat ataagcgaaa aagagtccgg gattttttgc tcgccagaca
    15841 tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc
    15901 catgcttcgc ggaaacaaaa tatcggaata aggaaaaaaa cggcaacgac aaaaaactgc
    15961 tgtacatcca taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga
    16021 cggcgggata cccctgcgca tcttccggca cctggtgcat agccggaata gggaccagcg
    16081 gccctaaaaa acgcggttca cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg
    16141 cggcaaattc gcgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact
    16201 gagcctgaat ccccatatgc agcgcaataa ataatgcccg ctcacagtgg aaacgttggg
    16261 tgataataat gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg gaatccagcg
    16321 tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc gacaccagca gcgattaaat
    16381 ctttgcgcat ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata
    16441 gataatttac cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat
    16501 actgattaat tacgccagta cgataatatt ttgctgttcc gagcaccaca ccgacctggc
    16561 ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc
    16621 gatcgaggcc gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca
    16681 cgcgctttaa catgcaggag actcgatcta aggaaaattt ccatcaggct actttacccg
    16741 ctggttttgc gcaagaaacg gcaaactgtt tgccgggaat ttcgacacca ggagagggct
    16801 ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagcactcg
    16861 gtcgatactg tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa
    16921 cggcgcatcg gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt
    16981 gagattcaac agaattaacg ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt
    17041 ttcagcgtag ctgccactga aaatacgacc gtctttacat tccagcgcga caccgcttgg
    17101 cgacttactg taaggcatgt gcgaacggtt tgccgctgca atcgccgcct gagaaagcgc
    17161 atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc agcagcgttt taatctccag
    17221 atctttcggc ccaaaggcat ccggcagata gtcacgcagc gcgtgtgcct cgcggcccgg
    17281 cagatgaata cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc
    17341 acaaggcgtg tagttaacgg tgatggctgc aagcgctttt tcaccactca accaggcgtg
    17401 gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt gtcgcaccaa taaattccat
    17461 attggcaccg aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt
    17521 cgacaatggc gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc
    17581 ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg
    17641 gaagtacttg tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc
    17701 aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca
    17761 gtttacgaac ccgttatggc tttatatgtg atctgaatct cattaattat gcaacgcgtg
    17821 aaacttacat caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat
    17881 cgggaaaagg aacggtgcga tcagcgaagt aattatcccg cataacacca gcgctagcga
    17941 actaaatgca ccttcctgat aatccagctc ggcgcagcgc gccgtaccga gggcgtgcga
    18001 ggcagtcccc attgccagac cgcgcgcagc tttggtacga atacgcatcg cattaagcaa
    18061 tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc acgcaaactg cgctgattgc
    18121 cggaataccg ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg
    18181 caggattgac gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt
    18241 caccatcgca accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg
    18301 gatctggtgt agctgctcat ataaaggata ggccagcgcg acgaccgccg gttgcagcag
    18361 atcgtttagc acttcgctac ctttaaagta gctgtcgtaa gagatgccgg tcagcattaa
    18421 aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa acttataccg
    18481 tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg accaccagat
    18541 attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc gtgtaccagt
    18601 tgcgaactcc agctcaccac cagaaaaacc accagcgtac tgattgcaca ggaaaccact
    18661 accgggccaa actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc
    18721 acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac ccatttcgcc
    18781 ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga
    18841 atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg
    18901 acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca
    18961 gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa
    19021 tcagcgctta ttttttcgct ccccggtatt acgcaatgag cagcgaactc aggcatacat
    19081 caagcataga gagggattgc agtttgcagc aatccctctt tgcagacctt taatccgttg
    19141 accataggcg ctaacatagc gcctcatttt tttgcgggtg atgatatgcg tgtgttactg
    19201 gcaccgatgg agggagtgct tgactctctg gtgcgtgaat tgctgaccga agttaacgac
    19261 tacgatctgt gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc
    19321 tttcatcgca tttgccctga gctacaaaac gccagccgga caccatctgg tacgctggtg
    19381 cgcgtgcagt tgttaggtca gttcccacaa tggctggcag agaacgccgc ccgtgcggtg
    19441 gagttaggtt cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac ggtcaacggt
    19501 agcggtggtg gggcgacgtt actcaaagat cctgaactca tctaccaggg tgcaaaagcg
    19561 atgcgtgaag ctgtaccggc gcatttgccc gtcagcgtga aagtgcgtct gggctgggac
    19621 agcggtgaga agaaatttga aatcgccgat gcggttcaac aggctggtgc tacggagctg
    19681 gtggtgcatg gacggacaaa agagcagggt taccgcgcgg agcatattga ctggcaggcg
    19741 attggcgaga ttcgccagcg gctgaatatt ccggtgattg ccaacggtga aatctgggac
    19801 tggcagagtg cgcaacaatg tatggcgatc agcggctgcg acgcagtgat gattggtcgc
    19861 ggggcgctca atattcccaa cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg
    19921 tggccggagg tggttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc
    19981 gggttatatc acgttgcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat
    20041 gaagcaacgg aattatttca gcatgttcgg gtgttgaata attcccctga tattgcaagg
    20101 gctattcagg caattgatat cgagaaactc taaaaaacca tagtgatgtt ttttgacaat
    20161 gtgttagctc tttatcaata ggccgcacgg ttgtttggtg gcgtcaaaat
//
LOCUS       DAZFVO020000091        20037 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFVO020000091 DAZFVO020000000
VERSION     DAZFVO020000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 20037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.091"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1048
                     /locus_tag="ACHKNB_RS15180"
                     /old_locus_tag="pgaptmp_003036"
     CDS             <1..1048
                     /locus_tag="ACHKNB_RS15180"
                     /old_locus_tag="pgaptmp_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGU4196504.1"
                     /translation="DNVAFEAFKAGAFDLRMENDAKNWATRYTGKNFDKKYIIKDEQK
                     NESAQDTRWLAFNIQRPVFSDRRVREAITLAFDFEWMNKALFYNAWSRTNSYFQNTEY
                     AARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPVSKGDGYDRDNLLKADKLLNEAGWV
                     LKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQHSLQRLGINMDIRKVDNSQITNRM
                     RSRDYDMMPRVWRAMPWPSSDLQISWSSEYINSTYNAPGVQSPVIDSLINQIIAAQGN
                     KEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAWWDKFSQPAVRPVYSLGIDTWWYDV
                     NKAAKLPSASKQGE"
     gene            1049..2143
                     /locus_tag="ACHKNB_RS15185"
                     /old_locus_tag="pgaptmp_003037"
     CDS             1049..2143
                     /locus_tag="ACHKNB_RS15185"
                     /old_locus_tag="pgaptmp_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HGU4196505.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            2143..3168
                     /locus_tag="ACHKNB_RS15190"
                     /old_locus_tag="pgaptmp_003038"
     CDS             2143..3168
                     /locus_tag="ACHKNB_RS15190"
                     /old_locus_tag="pgaptmp_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4196506.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            3170..4759
                     /gene="yejF"
                     /locus_tag="ACHKNB_RS15195"
                     /old_locus_tag="pgaptmp_003039"
     CDS             3170..4759
                     /gene="yejF"
                     /locus_tag="ACHKNB_RS15195"
                     /old_locus_tag="pgaptmp_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HGU4196507.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(4763..5115)
                     /locus_tag="ACHKNB_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /pseudo
     CDS             complement(4763..5115)
                     /locus_tag="ACHKNB_RS15200"
                     /old_locus_tag="pgaptmp_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
     gene            complement(5448..6638)
                     /gene="bcr"
                     /locus_tag="ACHKNB_RS15205"
                     /old_locus_tag="pgaptmp_003041"
     CDS             complement(5448..6638)
                     /gene="bcr"
                     /locus_tag="ACHKNB_RS15205"
                     /old_locus_tag="pgaptmp_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HGU4196508.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(6666..7361)
                     /gene="rsuA"
                     /locus_tag="ACHKNB_RS15210"
                     /old_locus_tag="pgaptmp_003042"
     CDS             complement(6666..7361)
                     /gene="rsuA"
                     /locus_tag="ACHKNB_RS15210"
                     /old_locus_tag="pgaptmp_003042"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HGU4196509.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            7510..9270
                     /locus_tag="ACHKNB_RS15215"
                     /old_locus_tag="pgaptmp_003043"
     CDS             7510..9270
                     /locus_tag="ACHKNB_RS15215"
                     /old_locus_tag="pgaptmp_003043"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGU4196510.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            9395..9679
                     /gene="rplY"
                     /locus_tag="ACHKNB_RS15220"
                     /old_locus_tag="pgaptmp_003044"
     CDS             9395..9679
                     /gene="rplY"
                     /locus_tag="ACHKNB_RS15220"
                     /old_locus_tag="pgaptmp_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HGU4196511.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(9818..10825)
                     /gene="yejK"
                     /locus_tag="ACHKNB_RS15225"
                     /old_locus_tag="pgaptmp_003045"
     CDS             complement(9818..10825)
                     /gene="yejK"
                     /locus_tag="ACHKNB_RS15225"
                     /old_locus_tag="pgaptmp_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HGU4196512.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            11007..11234
                     /locus_tag="ACHKNB_RS15230"
                     /old_locus_tag="pgaptmp_003046"
     CDS             11007..11234
                     /locus_tag="ACHKNB_RS15230"
                     /old_locus_tag="pgaptmp_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HGU4196513.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            11254..13014
                     /gene="yejM"
                     /locus_tag="ACHKNB_RS15235"
                     /old_locus_tag="pgaptmp_003047"
     CDS             11254..13014
                     /gene="yejM"
                     /locus_tag="ACHKNB_RS15235"
                     /old_locus_tag="pgaptmp_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HGU4196514.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            13089..13165
                     /locus_tag="ACHKNB_RS15240"
                     /old_locus_tag="pgaptmp_003048"
     tRNA            13089..13165
                     /locus_tag="ACHKNB_RS15240"
                     /old_locus_tag="pgaptmp_003048"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13123..13125,aa:Pro,seq:ggg)
     gene            13384..14625
                     /locus_tag="ACHKNB_RS15245"
                     /old_locus_tag="pgaptmp_003049"
     CDS             13384..14625
                     /locus_tag="ACHKNB_RS15245"
                     /old_locus_tag="pgaptmp_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HGU4196515.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLIKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALIRAGTDPQDHERQKRETEARR
                     QGNTFENVATAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRINEVMDYAANSGMIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            15132..15338
                     /locus_tag="ACHKNB_RS15250"
                     /old_locus_tag="pgaptmp_003050"
     CDS             15132..15338
                     /locus_tag="ACHKNB_RS15250"
                     /old_locus_tag="pgaptmp_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4196516.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTTA"
     gene            complement(15460..15663)
                     /locus_tag="ACHKNB_RS15255"
                     /old_locus_tag="pgaptmp_003051"
     CDS             complement(15460..15663)
                     /locus_tag="ACHKNB_RS15255"
                     /old_locus_tag="pgaptmp_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196517.1"
                     /translation="MTNKESTNTPSQKTNREHENMALEHESERFAPCAFVLDEFLKKY
                     SRSERMKMAAQYGPNKQDNCPPA"
     gene            complement(15721..15900)
                     /locus_tag="ACHKNB_RS15260"
                     /old_locus_tag="pgaptmp_003052"
     CDS             complement(15721..15900)
                     /locus_tag="ACHKNB_RS15260"
                     /old_locus_tag="pgaptmp_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4196518.1"
                     /translation="MPTGTTNNKSQQKTARVPLDVLSEMNLVKEENETDAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            16085..16285
                     /locus_tag="ACHKNB_RS15265"
                     /old_locus_tag="pgaptmp_003053"
     CDS             16085..16285
                     /locus_tag="ACHKNB_RS15265"
                     /old_locus_tag="pgaptmp_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4196519.1"
                     /translation="MVAVNHTPRLAHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            16275..16535
                     /locus_tag="ACHKNB_RS15270"
                     /old_locus_tag="pgaptmp_003054"
     CDS             16275..16535
                     /locus_tag="ACHKNB_RS15270"
                     /old_locus_tag="pgaptmp_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196520.1"
                     /translation="MHNKTTPDAAAAALTTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            16508..16729
                     /locus_tag="ACHKNB_RS15275"
                     /old_locus_tag="pgaptmp_003055"
     CDS             16508..16729
                     /locus_tag="ACHKNB_RS15275"
                     /old_locus_tag="pgaptmp_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196521.1"
                     /translation="MRDDRFNSLKQEFSGVSDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEVILRKATDGD"
     gene            16731..16964
                     /locus_tag="ACHKNB_RS15280"
                     /old_locus_tag="pgaptmp_003056"
     CDS             16731..16964
                     /locus_tag="ACHKNB_RS15280"
                     /old_locus_tag="pgaptmp_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196522.1"
                     /translation="MRDIYHETTDRVFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVIMELQEIDQLHDRYNEQHTGE"
     gene            16970..17269
                     /locus_tag="ACHKNB_RS15285"
                     /old_locus_tag="pgaptmp_003057"
     CDS             16970..17269
                     /locus_tag="ACHKNB_RS15285"
                     /old_locus_tag="pgaptmp_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4196523.1"
                     /translation="MKFKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQAVPRNVPTSREKARANIQKMKNMINGFRGKK"
     gene            17266..19020
                     /locus_tag="ACHKNB_RS15290"
                     /old_locus_tag="pgaptmp_003058"
     CDS             17266..19020
                     /locus_tag="ACHKNB_RS15290"
                     /old_locus_tag="pgaptmp_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HGU4196524.1"
                     /translation="MKLAPNVKQQPRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGEQQLSELDKLQIVPEGRKSVRIFRAGYLAPVMIKAIGQKLAAAGVQDA
                     NFYPDGMHGQKVENWREYLASERQNLSDGLVIELPVKQKAQLSQMADSERAQLLAGRF
                     DGVCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAE
                     IPVIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDECDAFL"
     gene            19309..19587
                     /locus_tag="ACHKNB_RS15295"
                     /old_locus_tag="pgaptmp_003059"
     CDS             19309..19587
                     /locus_tag="ACHKNB_RS15295"
                     /old_locus_tag="pgaptmp_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196525.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKNVEVES"
     gene            19584..20006
                     /locus_tag="ACHKNB_RS15300"
                     /old_locus_tag="pgaptmp_003060"
     CDS             19584..20006
                     /locus_tag="ACHKNB_RS15300"
                     /old_locus_tag="pgaptmp_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4196526.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLTCSQADDGT
                     ATMWLSVLAFGRQADALAKHRKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         5166 a         5019 c         5120 g         4732 t
ORIGIN      
        1 tgataatgtc gcctttgaag cgtttaaagc aggtgccttt gatttgcgta tggaaaacga
       61 cgctaaaaac tgggccacgc gttataccgg taaaaatttc gataaaaaat acatcatcaa
      121 agatgagcaa aagaacgaat cagcccagga tacgcgctgg ctggcgttta atatccaacg
      181 tccggtattc agcgatcgcc gggtccggga agcaatcacc ctcgcctttg actttgaatg
      241 gatgaacaag gcgttgtttt acaatgcctg gagtcgcacg aacagttatt ttcagaatac
      301 cgaatacgcg gccagaaatt accccgacgc cgcggagctg gtgcttctgg caccaatgaa
      361 aaaagatcta ccgccagaag tcttcacaca aatctaccag ccgccggtat ctaaaggcga
      421 tggctacgat cgtgacaacc tgttaaaagc cgacaaactt ctcaacgaag cgggctgggt
      481 gctgaagggt cagcaacgcg ttaatgccac aacgggtcag ccactcagct ttgaattatt
      541 gcttcccgca agcagcaata gtcagtgggt attgccgttc cagcacagcc tgcaacggct
      601 gggtatcaac atggacattc gcaaggtgga taactcgcaa atcaccaacc gcatgcgcag
      661 tcgcgactat gacatgatgc cgcgcgtatg gcgggcgatg ccgtggccca gttccgattt
      721 acagatttcc tggtcatcgg aatatatcaa ttccacttat aatgcccccg gcgtgcaaag
      781 tccggttatc gactcgctga tcaatcaaat tattgccgcg cagggaaata aagaaaaatt
      841 actgccgtta gggcgagcac tggatcgcgt attaacgtgg aattattaca tgctgccaat
      901 gtggtacatg gcggaagacc gtctcgcctg gtgggataaa ttctcccaac ccgctgtacg
      961 ccctgtttac agcctgggta tcgatacctg gtggtatgac gttaataaag cggccaaact
     1021 gccgtccgcc agcaaacagg gagagtagat gggcgcttac ctgattcgcc gtctgttgct
     1081 ggtgatccca acattatggg cgattatcac catcaacttt ttcatcgtgc aaattgcgcc
     1141 tggcggtccg gtcgaccagg ccatcgccgc cattgagttt ggtaatgccg gagtattacc
     1201 cggcgcaggc ggtgaaggtg ttcgtgccag ccatgcgcaa acgggtgtcg gcaatatcag
     1261 cgacagtaat taccgtggcg gacgcggatt agatccagaa gtgatcgctg agatcactca
     1321 tcgctacggt tttgataagc cgatccacga acgttacttc aaaatgctct gggactacat
     1381 ccgctttgat tttggcgata gcctgtttcg cagcgcctcg gtgctgacgc tgattaaaga
     1441 cagtctgccg gtttccatca ccctcggatt gtggagcacg ctgattatct atctggtgtc
     1501 gattccgtta ggcattcgca aagctgttta taatgggagc cgctttgacg tctggagtag
     1561 cgcatttatc atcatcggct acgccattcc ggcctttttg tttgccatcc tgctgattgt
     1621 cttcttcgcg ggcggcagct atttcgacct gttccctcta cgcggcctgg tttccgctaa
     1681 ctttgattcg ctgccgtggt atcagaaaat caccgattat ctgtggcata tcacgctgcc
     1741 ggtgctggcg acagtgattg gtggctttgc ggcgctgacc atgctgacca aaaactcatt
     1801 ccttgatgaa gtgcgcaagc aatacgtggt gaccgcgcgt gcgaaagggg taagtgaaaa
     1861 aaatattctc tggaaacatg tgttccgcaa cgccatgctg ctggtgattg ccggttttcc
     1921 ggcgacgttt atcagcatgt tttttaccgg ctcgctgctg attgaagtga tgttttcact
     1981 caatggcctt ggcttactgg gctacgaagc gaccgtctcg cgcgattatc ctgtaatgtt
     2041 tggtaccttg tatattttca ccctgattgg cctgctgctg aatattgtca gtgatatcag
     2101 ctatacgctg gtcgatccgc gtatagattt tgagggacgt taatgtcgcg actcagcccc
     2161 gtcaatcagg cccgttgggc gcgttttcgc cataaccgtc gcggctactg gtcgttatgg
     2221 attttcctcg ttttgtttgg tttgagtttg tgttctgaac ttatcgccaa cgataaaccg
     2281 ttgctggtac gttatgacgg cagttggtat ttcccgttgt tgaaaaacta cagcgaaagc
     2341 gattttggcg gcccgctggc aagtcaggct gattatcagg acccgtggct gaaacaacgg
     2401 ctggaaaata acggctgggt actgtgggca ccgattcgct ttggtgctac cagtatcaac
     2461 tttgctaccg ataagccctt cccttctcca ccctcccggc aaaactggct gggaacggat
     2521 gccaacggcg gcgatgtgct ggcacgcatt ctctatggca cgcggatctc ggttctgttt
     2581 ggcctgatgc tgactctctg ttccagcgtg atgggcgtgc tggcgggggc gctacaaggc
     2641 tattacggcg gtaaagtcga tctctgggga caacgcttta ttgaagtatg gtcggggatg
     2701 ccgacgctgt ttttgattat tttactttcc agcgtcgtac agcctaactt ctggtggctg
     2761 ctggcaatta ctgtcttgtt tggctggatg agtctggtcg gcgtggtgcg ggcggagttt
     2821 ttacgtactc gtaatttcga ctacattcgt gcggcacagg cgcttggcgt cagcgatcgc
     2881 agtatcatcc tgcgtcatat gttgcctaat gccatggtcg cgaccctcac ctttttaccg
     2941 tttattttat gtagttcgat aaccaccctg acctcgctcg atttcctcgg cttcggtctg
     3001 ccgctcggtt caccgtcact cggtgaactg ctgttacaag ggaaaaataa ccttcaggct
     3061 ccgtggcttg ggatcaccgc cttcttgtcg gtggcgatat tattgtcttt gctgatcttt
     3121 attggtgaag ccgtccgcga cgcatttgat cctaataagg cggtgtagca tgacgcaaac
     3181 tctgttagcg attgaagatt tgtcggtggg ttttcgccat cagcaaaccg tacgtacagt
     3241 agtcaatgat gtttcactac agattgaggc tggcgaaacg ctggcgctgg tgggtgagtc
     3301 aggttcaggc aaaagcgtta ccgcgctgtc aattttacgc ctgctccctt ccccgccggt
     3361 tgaatatctc tccggcgata ttcgttttcg tggcgaatcg ctgcttcatg ccagcgatca
     3421 aacgttgcgc ggtgtacgcg gtaataagat cgccatgatt tttcaagagc cgatggtgtc
     3481 gttaaatcca ttgcataccc tggaaaaaca gctttatgaa gtgctttcac tccaccgcgg
     3541 gatgcgtcgg gaagcggctc gaggcgaaat tcttaactgc cttgatcgcg ttggtatccg
     3601 ccaggcggca aaacggctga cagattatcc gcatcagctc tccggcggcg aacgccagcg
     3661 ggtgatgatt gcgatggcgc tgttaacgcg accggaatta ttaattgccg atgaaccgac
     3721 caccgcactg gacgtctctg tccaggcgca gattttacag ctgttgcgcg aactgcaagg
     3781 cgagctgaat atgggcatgc tgtttattac tcataacctc agcattgtca gaaaactggc
     3841 ccaccgcgtg gcggtaatgc aaaacggtcg ctgtgtcgag caaaataacg ccgctacgct
     3901 atttgcctca cccactcatc cttacacaca aaagctactc aacagtgaac cgtcaggcga
     3961 cccggtgccg ttgccagaac ctgcctcaac gttgctggat gttgaacagc ttcaggttgc
     4021 cttccccatt cgcaaaggga ttttgaagcg cattgtggat cataatgtgg tggtgaaaaa
     4081 catcagtttt acgctacgag cgggtgaaac actgggttta gtgggcgagt ccggttccgg
     4141 gaaaagtacg acgggactgg cgctgctgcg actgattaat tctcagggca gcatcatctt
     4201 tgacggtcag ccactgcaaa atttaaatcg ccgccagctg ttacctattc gtcatcgcat
     4261 tcaggtggta tttcaggatc caaactcctc gctcaaccca cgactcaacg ttttgcagat
     4321 tattgaggaa ggcttacggg ttcaccagcc gacgctttct gccgcacaac gcgaacaaca
     4381 agtgatagcc gtgatgcatg aagtgggatt agatcctgaa acacgccacc gttatccggc
     4441 ggagttctct ggtggtcagc gacaacgtat tgcgattgcc agggcattaa ttcttaagcc
     4501 ctcgctgatc atacttgatg aaccgacatc atcactcgac aaaacggtac aggcgcaaat
     4561 attgacgcta ttgaaatcat tgcaacaaaa gcatcaactg gcctatttgt ttatcagcca
     4621 cgatttgcac gttgtccgcg cgttatgtca tcaggttatc gtactgcgac aaggggaagt
     4681 agtggaacaa ggaccgtgcg cgcgcgtgtt tgccacaccg cagcaggagt atacgcgtca
     4741 gctactggcg ttgagctgac gcttaaaaag gattgtagtt tgaaaagggt tcggcaattg
     4801 ccacaccaaa atttttcaat cggcatgttg cagcaaactc gtcctggcga tttacaaaca
     4861 ggcacggctc accttcacac tccagcaccg aacatggaac ttcgatgtcg cttaacgcct
     4921 ggcttagttt atacatcacc gaccatgcat tatcgccatc cgggctaagc aatttaagcg
     4981 ccaccaggct gttgtcaccg cacggtccat tttgcggaat cggttcaacc ttcgaacctt
     5041 cgaacctgcg aaaccagcag accagcgata gttctgcggc agtcgatgga cgattgagag
     5101 ttgtaatgaa gtcacttatt ttccccggaa gtacattcac ttcacaattt gtttacatca
     5161 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
     5221 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
     5281 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
     5341 acataaattg attttacata aaataaacat atatcgggga aatgatagat ttgtggttga
     5401 catgcatcaa caaaggaagc cttttacctt cctcgttgtg caatagatca ccgttttttc
     5461 ggccgactgg cgtacagaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa
     5521 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
     5581 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
     5641 tcaagaatga ccgccatcgc attggatgac accatcgaca cgcagcccac aaacgccgca
     5701 acgccaacca ccagcgacca gaatcccagc cccagcagcg cactgatgac catccacgct
     5761 gccataataa attgtatcca caaccccgag cggaacatat ttaacgcgcc aatgcggcga
     5821 acgaagcggc tgttaaagat ggtcatcacg aacaggaaaa caatgtttag tgcaaagtaa
     5881 taaccaaagt tttccggcgc gacgtggtta atttcaatgt agacaaacgg tccggcgctc
     5941 aggaacgaga acattccggc aaagctgaaa ccgctggcca gcatataact caggacacgc
     6001 ttatgacgga acagcgccgc aaagttacca atagtggtac gaatgtgaaa tggctgacga
     6061 cgctccggtg gtaaggtttc tttaatcagg aagaaaatca ttgccgaagc cagaatcgcc
     6121 gctaatgcca ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccaaccgccg
     6181 acaatcggtg ccatcaacgg tgcaatagtg gtcaccagca tgacaaacga catcatccgc
     6241 gagaactctt ctttcgggta aatatcgcgc atcaaagcgt tgataaccac gctggccgca
     6301 gccgccgcca gcccgtggaa gaaacgcatc acaatcagct gatcgatggt gtttgccaac
     6361 gcacacgcca ccgcggcggc ggcaaacacc agcgtaccgc cgagcaccac cggcttacgc
     6421 ccgaagctgt ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa
     6481 gtactgaggg tcatctgcgt actgcccgca ggtacgccaa actgcgctga aattaccggt
     6541 agcgcgggca gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata
     6601 aaaacaatag caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcatgg
     6661 aaaagttaga cgacgctggc aatttcttct tcagttaacg gacgatattc accgggggct
     6721 aaatcagcat ccagcgtaat accgccaata cgttcacgat gcagctcaac cacgtggtta
     6781 cccacggcgg cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga
     6841 acctgcgttg gggtaatcac ttccagcacc gcaggcttag tgagatcttt ttcgttatgc
     6901 agctgcacgc ctttagcaaa ttgctctgcc gtatcgtcag ctacaggtga ttccagtgtc
     6961 accagatagg tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga ccactgacca
     7021 tcatcagtca tcagcaccag accggtggta tcgatatcca gacgtcctgc ggcgtgcagt
     7081 ttccaggcta ccggttcatc cagaaaatag agcaccgttg ggtgatcagg gtcgtccgtg
     7141 gagcaaacat agccctgagg cttattgagc atgaagtaac gtggaccatg ttgctgcgcc
     7201 agcgggttgc catcgtaagc gacatcatgt tcaggaagca gtttgaacgc tgcattacga
     7261 acaatttcgc catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg
     7321 ctaacgccga gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta
     7381 aaaacggagg tcgggcattg acccgaaaat ctgaactgtt gtctgcccag tatagcggtc
     7441 taataacatc cctcaaggga aaaagatcca tggcatacta ttagcagaat aatctaccta
     7501 cgcgagacca tgatttttac actccgccca tatcagcaag aagccgtgga tgccacgctc
     7561 aaccattttc gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg cgcaggtaaa
     7621 agcctggtga ttgccgaact ggcacggctg gcgcgtggtc gtgttctggt gctggcacac
     7681 gtcaaagaac tggtggcgca aaaccatgca aagtatcagg cgctggggct ggaagccgat
     7741 atttttgccg ctgggttaaa gcgcaaagag agccacggta aagtggtatt tggcagcgtg
     7801 cagtcggtcg ctcgtaatct tgatgccttt cagggtgaat tttcgctgtt gattgtcgat
     7861 gaatgccacc gtattggtga cgatgaagag agccagtatc agcaaatcct cactcacctg
     7921 actaaagtga atccccactt acgcctgctg gggctgactg ccacgccttt tcgattgggc
     7981 aaaggctgga tctaccagtt ccattatcac ggcatggtac gcggcgatga gaaagccctt
     8041 ttccgtgact gcatttatga gctgccgctg cgttatatga ttaaacacgg ctatctgacg
     8101 ccgccagaac gactggatat gccagtagtg caatacgatt tcagccgctt gcaggcacag
     8161 agtaacgggc tgttcagcga agctgatctc aaccgtgagc tgaaaaaaca gcaacgcatt
     8221 acgccacata tcatcagcca gatcatggag ttcgccgaaa agcgcaaagg ggtaatgatt
     8281 ttcgccgcca ccgttgaaca cgcaaaagag attgtgggat tactgcctgc cgaagatgct
     8341 gcactgatta ctggcgacac ccccggcgct gagcgcgatg tgttaattga agattttaaa
     8401 gcccagcgtt ttcgctatct ggtcaacgtc gcagtactga ccaccggatt tgacgccccg
     8461 cacgtcgatc ttatcgccat tctgcgccct accgaatcag tgagtcttta ccaacaaatt
     8521 gtcgggcgcg gtctgcgtct cgctccgggc aagactgatt gtttaattct tgattatgcg
     8581 ggtaatcctc acgatctcta cgcgccggaa gttggtacac cgaaaggcaa aagtgacaac
     8641 gttccggtac aggttttctg ccctgcctgc ggttttgcca acaccttttg ggggaaaacg
     8701 accgccgacg ggacattgat tgaacacttt ggtcgccgct gtcagggatg gtttgaagat
     8761 gacgacggtc atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg cccgcaatgt
     8821 aacgcagaaa atgatattgc cgctcgccgc tgccgcgaat gtgacacggt actggttgat
     8881 ccggatgata tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt acgctgtagc
     8941 ggcatgtctt tgcagcatga gcacgacgag aaaggcgaat ggttgaaaat cacctattac
     9001 gatgaagacg gcgcggatgt gagtgagcgt ttccgtctgc aaacgcccgc ccagcgaact
     9061 gccttcgagc agctttttat ccgcccgcat acgcgcacac cgggcatccc tctgcgctgg
     9121 atcaccgccg ccgatatcct cgcccagcaa gccttattgc gacacccgga ttttgtcgtt
     9181 gcccgcatga aaggtcagta ctggcaagtg cgtgaaaaag tgttcgatta cgaaggtcgt
     9241 tttcgtcggg cgcacgaatt acgcggttaa tggtagtttt gattgatgta taaagcgatt
     9301 gagtatagaa tctcgcccgc ttttgcatac gcaaagcaga tcacttacct gttgctgggt
     9361 cgcctgtagc aggattaatt taagagagaa agaaatgttt actatcaacg cagaagtacg
     9421 taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt tcccggcaat
     9481 catctacggt ggcaaagaag caccgctggc tatcgagctg gatcacgaca aagtcatgaa
     9541 catgcaagct aaagctgaat tctacagcga agttctgacc atcgttgttg acggtaaaga
     9601 aatcaaagtt aaagctcagg acgtacagcg tcacccgtac aaaccgaagc tgcagcacat
     9661 cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc ggcggggttt
     9721 tttgtatctg cagattatgc ctgattacgg tattgctatt ttttgcaggc cagataaggc
     9781 gttcacgccg catctggcat tcagaacaca aaaccgatta attgccgcca gacgtccggc
     9841 gctgcaattg gtcacgcaaa ttcggcggcg tgcctttaat ggtcagggta tcggttgccg
     9901 gatcccagaa gatgcgttca cctaacagca tcgcatcaaa gttaatagtc aagccaccgc
     9961 cgctaccggc aaacttcgtc aactgacgca gtgtgctacg gtctgccggg aagctctctt
    10021 ccagttcgta gcctttttca gccgcaaact ccgtgaaact cacttcgctg acgcctgcca
    10081 gctctttcga cagcgactcc agttcgatct cttcgcctgc ctgcagctgc tcattacagt
    10141 agctataaac ctgctggcgc acgttctgtc gctctgcttt atccagttgt gcttcggcag
    10201 tgaaatcatc aaccgcttgc aacagcccac ggttttgcgc tttcgcgttt aacccttcgc
    10261 tggccccgag gaaatccata aagaaatccg ccactttgcg ccctactcgc cctttcagga
    10321 aagtgagata gcgggtggac tctggattgg tttcccattc ggttaaatcg atacgcgcaa
    10381 caatatccgc atgattgata tcaagataat gggttggatt gatgtcgaga ttttcgttaa
    10441 cgcgcatact gctcaggttg ctcagaactg ccaccagcag atactccacc gccagataac
    10501 ggtagtggca aaacagcacg aatccaccgt cagcaaaagg atatttcgct aattcgtcac
    10561 gcaggcgccc ggttgccgcg cggctaaatg ccagaaagtc ctcttcgccc tgacgctgta
    10621 agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa gccgtaagct ttatttttgg
    10681 cgctatagac ccgatgcagt tctgccacca tctcaacgac ggtttctgtc ggttccagca
    10741 atgaatcgcg caacaccagc tcaaggttct gctcatcacg cttgataagc tggtgcagag
    10801 caatctggtt gatatccaga ctcatgataa actctccttt aagaccgggc ggtattcaac
    10861 caccgccggt taagcgacgc aataaaagat ggcggtcatt ttacagaagg cagcgtaacg
    10921 agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa tatgttgccc
    10981 tttcatcaac taactgattt cgatttatgc cacaaatttc ccgctacagt gatgaacagg
    11041 ttgaacaatt gctcgcagag ctgctcaacg tactggaaaa acataaggct ccgaccgacc
    11101 tttccctgat ggtgttaggc aacatggtca ctaaccttat caataccagc attgccccgg
    11161 cccaacgcca ggcaattgcc aactcttttg cccgcgcctt acagtcctct atcaacgaag
    11221 acaaagcgca ctaagggaaa cagataacag gttatggtaa ctcatcgtca gcgctaccgt
    11281 gaaaaagtct cccagatggt cagttggggg cactggtttg cactgttcaa tattctgctt
    11341 tcgctcgtca ttggcagccg ttacctgttt atcgccgact ggccgacaac gcttgctggt
    11401 cgcatttatt cctacgtaag cattatcggc catttcagct tcctggtgtt cgccacctac
    11461 ttgctgatcc tcttcccgct gacctttatc gtcggctccc agaggctgat gaggtttttg
    11521 tccgtcattc tggcaacggc gggaatgacg ctattactga tcgatagcga agtctttact
    11581 cgtttccatc tccatcttaa tcccatcgtc tggcaactgg ttatcaaccc agacgaaaat
    11641 gagatggcgc gcgactggca gctgatgttc atcagcgtgc cggttatttt attgcttgaa
    11701 ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg tcggcgcttc
    11761 gcgcgcccgc tggccgcatt cttatttatc gcctttatcg cctcgcatgt ggtgtatatc
    11821 tgggccgatg ccaacttcta tcgcccgatc accatgcagc gcgctaacct gccgctttcg
    11881 tacccgatga cggcgcgacg ttttcttgag aagcatggtc tgcttgatgc gcaggagtat
    11941 caacgccgtc ttattgagca aggtaatcca gacgccgttt ccgttcagta tccgttaagc
    12001 gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac tgtcgatggc
    12061 ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat
    12121 atttcgttca cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg
    12181 ttctatggca tctcgccgag ctatatggac ggcattctgt cgacccgtac ccctgcggcg
    12241 ttaattactg cgcttaatca gcaaggctat cagctgggat tattctcatc agatggcttt
    12301 accagcccgc tgtatcgcca ggcattgttg tcagatttct cgatgccgag cgtacgcacc
    12361 caatccgacg agcagaccgc cacgcagtgg atcaactggc tgggccgcta cgcacaagaa
    12421 gataaccgct ggttctcgtg ggtctctttc aatggcacta acattgacga cagcaatcag
    12481 caggcatttg cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaccgc
    12541 gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggtggtgat tatcactgcc
    12601 ggtcggggta ttccgctgag cgaagacgaa gaaacctttg actggtccca cggtcatctg
    12661 caggtgccat tagtgattca ctggccaggc acgccggcgc agcgtattaa tgctctgact
    12721 gatcataccg atctgatgac gacgctgatg caacgcctgc tacatgtcag cacacctgcc
    12781 agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgtcatta ctgggttact
    12841 gcagcggata acgatacgct ggcaattacc accccgaaaa agacgctggt gctgaacaat
    12901 aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag
    12961 ttaagtttgt tattgcaagt gctgacagac gagaagcgtt ttatcgctaa ctgattaatt
    13021 ataaatcagt tagcaaaata tcttacttgc aatcggtgtg gaaaacggta gtattagcag
    13081 ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga
    13141 ggttcaaatc ctctcgtgcc gaccaaatcg cccaccggat accaccggaa atcaccgaat
    13201 aagacaggga tagcccgcca attacttgat tggcgggttt ttttatggtt tttagtcacc
    13261 gaacatcacc ggatttataa gctatcagcc ggatttttta gttatacgtt tagttatacg
    13321 ggctgacgta taactaaagc cgtataacta aaaccctgtt ttttcgtttg gaatcataag
    13381 catatggcga gaaaaacacc cccattaacc acagtacaga tcaaagcagc cagaccagca
    13441 gaaaaggaat acaccctaca ggacggcgga gggctttttc tcctgatcaa accgtccgga
    13501 tcaaaactct ggagattttc ctactaccga ccatcggaca aaaaaagaat attgctgagt
    13561 tttggatcgc ttgatgatgt ttccctggct gatgccagaa aacgccgtag cgagtacagg
    13621 gcgttaattc gtgccggaac tgacccgcag gaccacgaga ggcaaaaaag agagacagag
    13681 gcccgaagac aagggaacac gttcgaaaat gtggcaacag catggtacca ggtgaaaatc
    13741 agccagaatc tggcccccaa tacgattaaa gacatctggc gatcgctgga aaaatatgta
    13801 ttcccgttca tcggcaacac gccaatagac accctgaccg ctcgcaggtt cgttgaggtg
    13861 cttaccccca tcaaggaacg tggcaaccta gaaacgctca agcgagtttt acagcgtata
    13921 aatgaggtaa tggattacgc cgccaatagt gggatgattg atgccaatcc ggctatgaat
    13981 gtgcgtaagg cgttcccctc ccctgtaaaa aaacatatgc caacaatccg ccccgaacag
    14041 ctgccggagc ttatgcaggc tttatcagta tcggcaacag aacggcagac cagattactg
    14101 attgaatggc agttactgac cgtaacccgt cccgttgaag catcgtcggc acgctgggaa
    14161 gaaatcgaca tggaagcgca acgctggacc atacccgccg gacgcatgaa gatgcgccgc
    14221 gaccacgtta tcccgctttg tgaccaggct atggcagtgc tggaggctat gaaaccaatc
    14281 agccaccacc gcgattatgt tttcccgagt ctgaaagacc cacagcagcc gatgaacagc
    14341 cagaccgcta acgcagcatt acgacgcatg ggattcgctg gcgtgctggt gtctcatgga
    14401 ttacgcgcca tattcagcac agcagcgaac gaagaaggct ttgagccgga cgtaatagag
    14461 gccgcacttg cccacgtcga caccaacgaa gtcagacggg catacaaccg gagcaactac
    14521 atagaaaaac gcatcgtgct tatgcgctgg tggggcgaat ttgtcgaggc tgcggcgacg
    14581 ggcgtaaccc tcgccagtgg taaaaggggt atccgagccg tttagctgta cagaaaacca
    14641 gtaaaaacta cgaaaaccat ttaaaaccgt cgtataattg catcacattt aacgacgagg
    14701 ccttgaaaaa tgaaactgtt aagatgcaaa caaatttcag atgcaattgc gacgggctgc
    14761 aactggccct gatggttcag catgaatttt ggtcaaccta cgatccggag gacagaacga
    14821 cggccccatc aaaaaaacag gtagtagatt tcctggtatc ccgtggggct tccagaaatc
    14881 tggctgtaag tattgataag gttgtacgtc cggcatccat gaagaccgga ggcaggccaa
    14941 aaaaatggcg gtaacaatcc tgaaagcggc agaaatgccg ctttttttat aaatcccttt
    15001 caaatcatca atataaaaaa tggtgtatac cgtttaaaaa cggtgagtac tgtttttccc
    15061 tgcatccgat gatttaccgt atttctcacc ggaatacacc ggaatacacc ggattaatga
    15121 ggtaaatcac gatggaagca atcaaaaaag tcatctttcg ccaggaagta aaaaaactta
    15181 tccacattaa ggcagacagc acgctgcaaa gcatgatcaa cgccggagaa ttcccgcagg
    15241 gttttcgtgt cggtttgcgc cgtcgcggat ggtatgagga agacgtaatc gcatggatga
    15301 aagaacgcga acaagaagcg cgcggaacga ccgcttaaca tatatagggc aaatgatgaa
    15361 cataaaaaaa gcggccccga aatggagcca cctttctgaa caattaaccc gctgcgccgt
    15421 atgtgtcaac aatcataagc ccgggcatga taaccgcgat caggctggtg ggcaattatc
    15481 ttgcttgttg gggccgtatt gtgctgccat tttcatccgt tcagaacgtg aatatttttt
    15541 tagaaactca tcaaggacaa aagcgcatgg tgcgaatctt tctgattcat gctcgagcgc
    15601 catattttcg tgctctctgt tcgttttttg cgatggtgta ttggttgatt ctttgttggt
    15661 cattgtgtgt tcctgtaaag caatgcgccg gagtacctca caccacggcg ctgatagtga
    15721 ttattctgat tctttggcct tgcgacgctg gcggcgtttg atttcgccgc gcatggcagt
    15781 aacgataaat tgtgcgtctg tttcgttttc ttcttttacc aaattcattt cgcttaaaac
    15841 gtctagaggt actctagcag ttttttgttg tgacttgttg ttggttgtac ctgttggcat
    15901 ctctgttctc cttactgtta ggtggtgttc agtatacgga gaaaaaaaaa taaaaaaaga
    15961 cttgaagtgc aagtcaccta aaagtaacct agatttcaag gtgacttgca ccaaaaaaca
    16021 acgccccaca gtgctggaac acatgcaggg cgtctaacca caaacactat agagaggtaa
    16081 atttatggtt gccgtaaatc ataccccacg actagcacac acacaaacgg cctttgtgtg
    16141 gcgttttctg gcactgagtg ccggagaatc tcaaatcatc cacgtaaccg cctggacgga
    16201 acgcgaagcg cgtagccgtt gcccgtccgg ttgtgttgct gtatttgcag cccgtattcg
    16261 ccagggagaa acctatgcac aataaaacca caccggacgc agcagccgcc gcgctcacta
    16321 cgctgatgca cgcgcttatt gatatttctg ttattgctga cagggcgcat aagcacgcca
    16381 ccagtgaaac agaatatgca ggggctttcg ttcctcattc gctggcggtt atgcaactta
    16441 gtgctgatat ggcgctgaat gaggccaaag ctatcctgat tgctgattgt gaaaatgggg
    16501 gggggttatg cgtgatgatc gttttaattc cctgaaacaa gaattttccg gcgtttctga
    16561 tgatgcggct gatgcgcttt cgtcaatatc tgaaattatg cgggtggctt ttttctttct
    16621 ttgcactgat gagcacagag atacagggct aaatattctt gatattgccg ctaactatgc
    16681 tgatttcgtg acagaggtta ttttaagaaa agcgacggac ggggattaat atgcgtgata
    16741 tttaccacga aacaacagat cgcgtatttc ttgcactttc tcacagtgaa aatatgatgg
    16801 aaatattgcg catatggctt gaaacacttg gcgacaatga acgcgacaaa caaaaatcaa
    16861 gaattgtcac ggcattaata acgcttcttg agcctgtaat aatggaacta caagaaatag
    16921 accaattgca cgacagatat aacgaacagc acaccggaga ataaaaataa tgaaatttaa
    16981 atattctggc ttaactgcca gtggcaacac tcaccctaaa tttacgcgcg gtgatattta
    17041 ccgcgaccag tacggcggca cggtaatgat taagggcgtg gcgggacggt gcgtaactta
    17101 ccgccgtgaa ggttacgaat atgattgcgt gatgcctgtt tatcagttcc ggcgtgattt
    17161 ttctcttgtg caggcagtac cgcgtaacgt gcccaccagc agggagaaag cacgcgccaa
    17221 tattcagaag atgaaaaaca tgattaacgg attcaggggc aaaaaatgaa actggcaccg
    17281 aacgtaaaac agcagccacg cggcataaaa cacaaagaaa cagaagtcat tatttttgcg
    17341 ggtagtgatg cctggtcaca cgcgaaacaa tggcaggaac atgacgcgcg tatggccgga
    17401 gataatgagc ctcctgtgtg gcttggggag cagcagttat cagaactgga taagctgcaa
    17461 attgtgccgg aaggcagaaa atccgtgcgc atattcaggg ccggatatct tgcgccagta
    17521 atgataaagg cgattggtca gaagctggcg gcggcaggcg tacaggatgc aaatttttac
    17581 cctgatggta tgcacggtca gaaggtggag aactggcgcg aatatctggc cagtgagcgc
    17641 cagaatcttt ctgatgggct ggttattgag cttcctgtaa agcaaaaggc gcaactttcg
    17701 cagatggcgg acagtgagcg cgctcaactt cttgccggac gatttgatgg cgtttgcgtg
    17761 catcctgaaa gtgaaatcgt tcacgtatgg cgcggcgggg tatggtgtcc ggtcagcaca
    17821 atggaactta gccgcgaaat ggtggcgatc tattcagagc acagggccac tttcagcaag
    17881 cgcgtaatca ataacgccgt ggaagcgtta aaagttattg ccgaaccaat gggcgagccg
    17941 tccggcgatt tgctgccgtt tgccaatggt gcgcttgacc tgaaaacggg ggaattttcc
    18001 ccgcacacgc cggagaactg gatcaccacg cacaacggta ttgagtacac gccaccagca
    18061 ccaggggaga acatccgcga taacgcgcca aactttcata aatggcttga gcacgcagcc
    18121 ggaaaagacc cgcgcaagat gatgcggata tgtgccgcgc tgtacatgat tatggcgaac
    18181 cggtacgact ggcagatgtt tattgaggcc accggagacg gcgggagcgg taaaagtaca
    18241 ttcacacaca tagccagcct tctggcaggg aaacagaaca cggtaagcgc tgaaatgaca
    18301 tcgcttgatg atgctggtgg acgtgcgcag gttgtcggga gtcgccttat cgtcctggct
    18361 gaccagccga aatatacagg cgaaggaacg ggcatcaaga aaatcacggg cggtgacccc
    18421 gtggaaatta acccgaaata tgaaaagcgt tttacggcgg taatcagggc ggtggtgctg
    18481 gcgaccaaca acaacccgat gatattcacc gaacgggccg gaggtgtggc acgtcgtcgc
    18541 gtgattttcc gtttcgacaa tatcgtcagt gaggccgaaa aagacaggga gctaccggaa
    18601 aagattgcgg ctgaaatccc tgtcattatc cgccgattgc tggcgaactt taccgactct
    18661 gagaaagcac gggctttact actggaacag cgtgacggtg atgaagctct ggcaataaag
    18721 caacagacgg atccggttat tgagttttgc cagttcctga attttctgga ggaagcacgc
    18781 ggcctgatga tgggcggcgg tggcgattca gtgaagtaca cgaccagaaa cagcctttac
    18841 cgcgtctatc tggcgtttat ggcgtacgca ggcaggagca aaccgctaaa cgtaaatgac
    18901 tttggcaagg ctatgaagcc agccgcgaaa gtttacggac atgaatatat tacgcggaaa
    18961 gttaagggag taacgcagac taacgcaata acaacagacg agtgcgacgc gtttttataa
    19021 ttttttgtaa ttgctgtcta ccctgtctac ctgagtaaag aaagatacat ttaattcagt
    19081 gcattaagtt aggtagatag ccttttttta ctgtctaccc actatctacc ctctctacct
    19141 gattttacct gaatcagaca gggaggtaga cacggggtag atagcggata aaagcactct
    19201 accccgctga aagccgcgcc attactggca tgagggccac taaggtagat aaggtagaca
    19261 aggggaggca caactcaaaa ctttttaaac gagggggtaa aaataaatat gcacacttca
    19321 gggaaattta ataaatcact caaaaaacgc agagacagaa cagaaccgaa atatcgcgcg
    19381 ttagacatga cagagcacgc tttaaaggtg gcaatcagaa cgatagaccg ccacgcgggg
    19441 gaaggatacg cgaaagcaca tcccgaactg ataagcgcat tcatgaccac gacggcggca
    19501 aattttgcca cgctgacaga gcgggagatt gcggaagctg aacaggtgac aaccatcaac
    19561 gttaaaaacg tagaggtgga atcatgacag cacagatagc ggcttacggg cggctggtgg
    19621 ctgacccaca gttaaagacc accagcaagg gcacacaaat gacgatggcg agtatggcgg
    19681 ttcccctgac atgcagccag gctgatgacg gaacggcgac gatgtggtta tccgtcctgg
    19741 cgtttggcag acaggccgac gcactggcaa aacaccgcaa aggcgagctg gtgagcgtgg
    19801 cgggtaacat gcaggtaagc cagtggacag gccagaacgg cgaaacgcgg cagggctggc
    19861 aggttatcgc agacagcgta atcagtgcga gaacggcgcg accgggcggt aaaaaaggcc
    19921 agcaggggca ggctactgac gcactgaaca gggcaaaaca acaggcgggg aatgatgatc
    19981 cgtacgggga taacataccg ttttaagcaa cgagtgacag aagccggagc aatccgg
//
LOCUS       DAZFVO020000092        19944 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFVO020000092 DAZFVO020000000
VERSION     DAZFVO020000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.092"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(39..3920)
                     /gene="entF"
                     /locus_tag="ACHKNB_RS15305"
                     /old_locus_tag="pgaptmp_003061"
     CDS             complement(39..3920)
                     /gene="entF"
                     /locus_tag="ACHKNB_RS15305"
                     /old_locus_tag="pgaptmp_003061"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HGU4196527.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGG
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
     gene            complement(3917..4135)
                     /gene="ybdZ"
                     /locus_tag="ACHKNB_RS15310"
                     /old_locus_tag="pgaptmp_003062"
     CDS             complement(3917..4135)
                     /gene="ybdZ"
                     /locus_tag="ACHKNB_RS15310"
                     /old_locus_tag="pgaptmp_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HGU4196528.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4138..5340)
                     /gene="fes"
                     /locus_tag="ACHKNB_RS15315"
                     /old_locus_tag="pgaptmp_003063"
     CDS             complement(4138..5340)
                     /gene="fes"
                     /locus_tag="ACHKNB_RS15315"
                     /old_locus_tag="pgaptmp_003063"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HGU4196529.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5583..7823
                     /gene="fepA"
                     /locus_tag="ACHKNB_RS15320"
                     /old_locus_tag="pgaptmp_003064"
     CDS             5583..7823
                     /gene="fepA"
                     /locus_tag="ACHKNB_RS15320"
                     /old_locus_tag="pgaptmp_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HGU4196530.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQS
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7863..7961
                     /locus_tag="ACHKNB_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /pseudo
     CDS             7863..7961
                     /locus_tag="ACHKNB_RS15325"
                     /old_locus_tag="pgaptmp_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7998..8618
                     /gene="entD"
                     /locus_tag="ACHKNB_RS15330"
                     /old_locus_tag="pgaptmp_003066"
     CDS             7998..8618
                     /gene="entD"
                     /locus_tag="ACHKNB_RS15330"
                     /old_locus_tag="pgaptmp_003066"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HGU4196531.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8740..8892)
                     /gene="hokE"
                     /locus_tag="ACHKNB_RS15335"
                     /old_locus_tag="pgaptmp_003067"
     CDS             complement(8740..8892)
                     /gene="hokE"
                     /locus_tag="ACHKNB_RS15335"
                     /old_locus_tag="pgaptmp_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HGU4196532.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            8861..9130
                     /locus_tag="ACHKNB_RS15340"
                     /old_locus_tag="pgaptmp_003068"
     CDS             8861..9130
                     /locus_tag="ACHKNB_RS15340"
                     /old_locus_tag="pgaptmp_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196533.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPYLIN"
     gene            9334..10452
                     /gene="ybdK"
                     /locus_tag="ACHKNB_RS15345"
                     /old_locus_tag="pgaptmp_003069"
     CDS             9334..10452
                     /gene="ybdK"
                     /locus_tag="ACHKNB_RS15345"
                     /old_locus_tag="pgaptmp_003069"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HGU4196534.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10518..10766
                     /gene="ybdJ"
                     /locus_tag="ACHKNB_RS15350"
                     /old_locus_tag="pgaptmp_003070"
     CDS             10518..10766
                     /gene="ybdJ"
                     /locus_tag="ACHKNB_RS15350"
                     /old_locus_tag="pgaptmp_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HGU4196535.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            10831..11199
                     /gene="ybdF"
                     /locus_tag="ACHKNB_RS15355"
                     /old_locus_tag="pgaptmp_003071"
     CDS             10831..11199
                     /gene="ybdF"
                     /locus_tag="ACHKNB_RS15355"
                     /old_locus_tag="pgaptmp_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4196536.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11293..11946
                     /gene="nfsB"
                     /locus_tag="ACHKNB_RS15360"
                     /old_locus_tag="pgaptmp_003072"
     CDS             11293..11946
                     /gene="nfsB"
                     /locus_tag="ACHKNB_RS15360"
                     /old_locus_tag="pgaptmp_003072"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HGU4196537.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12054..13301
                     /gene="ybdG"
                     /locus_tag="ACHKNB_RS15365"
                     /old_locus_tag="pgaptmp_003073"
     CDS             12054..13301
                     /gene="ybdG"
                     /locus_tag="ACHKNB_RS15365"
                     /old_locus_tag="pgaptmp_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HGU4196538.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13369..14745)
                     /gene="pheP"
                     /locus_tag="ACHKNB_RS15370"
                     /old_locus_tag="pgaptmp_003074"
     CDS             complement(13369..14745)
                     /gene="pheP"
                     /locus_tag="ACHKNB_RS15370"
                     /old_locus_tag="pgaptmp_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HGU4196539.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(14847..17990)
                     /gene="cusA"
                     /locus_tag="ACHKNB_RS15375"
                     /old_locus_tag="pgaptmp_003075"
     CDS             complement(14847..17990)
                     /gene="cusA"
                     /locus_tag="ACHKNB_RS15375"
                     /old_locus_tag="pgaptmp_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HGU4196540.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18002..19225)
                     /gene="cusB"
                     /locus_tag="ACHKNB_RS15380"
                     /old_locus_tag="pgaptmp_003076"
     CDS             complement(18002..19225)
                     /gene="cusB"
                     /locus_tag="ACHKNB_RS15380"
                     /old_locus_tag="pgaptmp_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HGU4196541.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNISKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19241..19573)
                     /gene="cusF"
                     /locus_tag="ACHKNB_RS15385"
                     /old_locus_tag="pgaptmp_003077"
     CDS             complement(19241..19573)
                     /gene="cusF"
                     /locus_tag="ACHKNB_RS15385"
                     /old_locus_tag="pgaptmp_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HGU4196542.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(19606..>19944)
                     /locus_tag="ACHKNB_RS15390"
                     /old_locus_tag="pgaptmp_003078"
     CDS             complement(19606..>19944)
                     /locus_tag="ACHKNB_RS15390"
                     /old_locus_tag="pgaptmp_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGU4196543.1"
                     /translation="GRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLND
                     QISAQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNE
                     ISLYTALGGG"
BASE COUNT         4797 a         5484 c         5071 g         4592 t
ORIGIN      
        1 ttatttccta taattatggg ttaatagata atgttaattt acctgtttag cgttgcgcga
       61 ataatcggcc caattttttc aaacgcctct gaagatataa tatccacatg cgcacaatcc
      121 tgacgataaa tatccagctc ggctatccac ggcgaccagg cgcgttcggg actcatacct
      181 tcctgaagcg tgcgttcagc aacaaacagc gtcgctttac cgtcaaacgg tacgctatga
      241 gcagtcgtca gcaggcgcac tgcatcagcg tagttgcctt caatggtggt aaacaactcc
      301 gttgaagtac ctccctgctg tgccgccagg aaggcctcgc gttcgcggtt aatctccgcc
      361 agcacttccg ggtccagacc attagcttct ttttcctgcc agttttgcgt ttctggcggc
      421 caggtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca
      481 ataccctgca ccagcgtacc gccaagggaa taccccagca ggtaataagg gccgtgcggt
      541 tgttgctcaa gtaacgttgc cagatgcgct tcgcagactt catccaggtt tgtcgctgtc
      601 tgcatgggtc catgagggcg cggcgactga atgccaataa tcgaccattg tggatcgaga
      661 taacgcgaga gcacgctaaa ctgccaggca aaaccggatg ccggatggaa acaaaacagc
      721 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg ccgggagctg
      781 tcctcttcac catcaataat cgttgccagt ttggcgacgg ttgacgcgac catcacctgc
      841 cccggcgtca cctgacgagc aaactgtcga cttaactgcg ctgccagttt cattgccagt
      901 agcgaatgac cgccaagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc
      961 aacgacgcga atgccgcggc gataatcgtt tcactgcccg ctttcggtgc acgtcccgac
     1021 gcttgtgtct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
     1081 agaggtaact gtggaagttg cagcagaacc accggcacca tatgcggcgg caatgtttcg
     1141 cgaagctgtg cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag
     1201 cccaccaact gacgcgcatc accaccggtg gctgccgcca ggttaatcac acaggcgtgg
     1261 gtaacggctt gtttgacatc cggcagcgcc tgcatcacgc gatcgatttc gcccagttcg
     1321 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc caccgcgccg
     1381 ttatccagcc agcgggcgac gtctccggta cgatacatcc gttcacctgg ggcaaaagga
     1441 tcggcaataa aacggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttgg
     1501 atgccggtga gatagagatc acccgccaca cccggcggca ccggatgcat catcgcatca
     1561 agaatacgca gacccgtatt ccataccgga taaccaatcg gcacactgct gccgcgcacc
     1621 tgtgccagtt cctcgccaaa agccggatac cagctgacat ctaccgccgc ttccgtcggg
     1681 ccatacaggt tatgcaacgg cgcgccggtt aactgttgcc attcgcggca taaatcagcc
     1741 ggtaaggcct caccactaca gaaaacctgt ttcaacgtcg cgcaattctg gcgagcggtt
     1801 tgcggcgtca gcgatgcaac aaatgccgcc agcatcgacg gcacaaagtg cgtggtcgtt
     1861 acgccatatt cggcaaagaa ttgctgcata gcgagcgggt cacggtgcgc ttccggttca
     1921 gccatcacca gttttgcccc tgcgataaac ggccagaaaa actcccacac cgagacatca
     1981 aaactgcacg gcgttttttg ggcaacgaca tcttcgcctg taagcgggta gtgattttgc
     2041 atccataaca ggcggttaac gatagccgtc tgcccgacca ttaccccttt cggcctgccg
     2101 gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag ttgcagcggc
     2161 gcactgccct ggggggtaag cggggcgtta tagcaaaggc ttgttaaatt gggaacatcg
     2221 ctaaagcgcg gcagttgatc gtcggtggta attaacagcg acggacgcgc atcttccagc
     2281 atcattttca ggcgatcgtc cggatagccg gtatccagcg gtagccaggc cgcgccagct
     2341 tcaactattg catggagtgc cagggtcaaa aagaccgagc gcggtagtgc caccgccacg
     2401 ctgtcccctg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctcg
     2461 cgcatttccc gatagctgaa caggtaacgc gcatctgcca gcgccggagc atccggtgtt
     2521 tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc aacctgagtg
     2581 gcgttgatct gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac
     2641 aacagcgccg gatccgcggc gaactgggca atcagcattt tcaggcgttc agcatgctgg
     2701 attaacgttg gctcatcgta acgctgttta ttggcgagga tctcaatact caaatcaccg
     2761 tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc cagggtatgg
     2821 gtttgcgtct gaacaccagg aatatccagt tggtaatcaa ataccttgat attgagtacc
     2881 ggaccgaaca gcggttcctc tcctgccgct cgcccgctgt cacggacaat ttgttcggca
     2941 tcgtaacgtt gatgacgacg catttttttc agttgtgctg ccagtcgggt tgccagttcc
     3001 ggcagcgttt cttgcgccgc aatgtgaata cccaacggta aaacgttgag cacgggtccg
     3061 ctagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc ggcatagtcc
     3121 atgcgattgc acaatcgccc cagccacaag gctgccagcg caagggctaa atcggtacgc
     3181 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgtcggtaaa ttccagtttc
     3241 aggcgcagaa tatctgccga agcgctgcgc cccgctaaag gtgccggaga aagtgacgcg
     3301 ggcggcggca gttgacgacg ctgttctgcc cagaatgccg catcacgctg ccaggcttcg
     3361 ctttcgcggt attgctggta ctcttccact acatcagcga aaggcgtaaa cggcgaagca
     3421 ggcgttggtt cgccacgcag ccatgtgcag taaatattgg cgatctggcg agtaatggcc
     3481 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
     3541 tccgcaacat gtatcagctg atgaaagacc agtggtttac cgctatcgac gcgcaaatct
     3601 tgttgcaaat ccgccagcat taatgcctgc gcagtaccgt gcggatcaat attggttcgc
     3661 aggtcgataa tttctggcag ttcgaatatc agcgcatcat cgacccactg ccagacttcg
     3721 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc gacaaccacc
     3781 gcgcgggcca gtaatggcgc atcaacatct ccagttaact caacgtaatg cgccacgctc
     3841 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
     3901 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattggcc ggtgtcagag
     3961 tacgccagtg gacttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
     4021 tgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
     4081 tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca
     4141 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
     4201 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttatggg
     4261 gtgtaattgc gcatacagcg cctgattggc ccgcataatc atcggctcgc gaatacccgc
     4321 ttccagcaca atgcgcagac cttcggcgct aacttcacca gctttaagct tttcaagtaa
     4381 cacgccctct tgctgcccgc cccgatgcgg ccaccagtac gatcctgact ggcttaatac
     4441 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggacagcc caccaaaact
     4501 ctgcccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcaccag
     4561 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgggc
     4621 gcggtgcgtg gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa gttgctgacg
     4681 atgggtcagc gaagtcagca caggccagac gggcatactt tgcgcccaaa attcgccatc
     4741 gagcaaaact gccagcggac gttcttctgc tgttgcatcg ccggtggtaa aaatccatac
     4801 acgacgtgaa tttttcaacc gttcactttt ccagataatt tctttggctg ctatttctgg
     4861 cgcttgcgga caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac
     4921 agcgtgccct cgcccgcctt tccagctttg tggattcagc ggatcggcta ttgcctgggg
     4981 taatagtttt cgccagcctt cacgtaattc gaggcgatcg ggggatggtg cagaaaaaat
     5041 gtcatcgcgt tcggtgggaa taaagcagta gctgccgcgc cagttggcat tgagttgtgt
     5101 cgtccactgc cagacatcag tacctgcaat tcgctgcatc gactggggct ggctgttctg
     5161 atgatgatcg gtcacaccag tgatgtagat ccatacgcgc tttatcgtcg agtattcttc
     5221 agaaccttgg ggatcacgcc accagaaagt gacctcaaac atttcgtcat tcagacgctg
     5281 ccattccggg ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac
     5341 accataaccc catgtttact gtgcaatttt tcattgattg cagaaatata ttgataatat
     5401 tattgataac tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt
     5461 tattaaccaa ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg
     5521 tcgtgttgag gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa
     5581 caatgaacaa gaagattcat tccctggcct tgttggtcaa tctggggatt tatggggtag
     5641 cgcaggcaca agagccgacc gatactcctg tttcacatga cgataccatt gtcgttaccg
     5701 ccgccgagca gaacttacag gcgcctggcg tttcgaccat caccgcagat gaaatccgca
     5761 aaaaccccgt tgcccgcgat gtgtcggaga tcatccgtac catgccaggc gttaacctga
     5821 ccggtaactc caccagtggt cagcgtggga ataaccgaca gattgatatt cgcggtatgg
     5881 gtccggaaaa cacgctgatt ttgattgacg gcaagccggt aagcagccgt aactcggtgc
     5941 gtcagggctg gcgtggcgag cgcgataccc gtggtgatac ctcctgggtg ccacctgaaa
     6001 tgattgaacg tattgaagtt ctgcgtggtc cggcagctgc gcgttatggc aacggcgcgg
     6061 cgggcggcgt ggttaacatc attaccaaaa aaggcagcgg tgagtggcac ggctcttggg
     6121 acgcttattt caatgcacca gaacataaag aggaaggtgc cactaaacgc accaacttca
     6181 gcctgaccgg tccgctgggc gacgaattca gcttccgctt gtatggcaac ctcgacaaaa
     6241 cccaggctga cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc ggaacgtatg
     6301 ccacgacgtt accagccggg cgcgaagggg tgatcaataa agatattaac ggcgtggtgc
     6361 gctgggactt cgcaccattg caatcgctgg aactggaagc gggttacagc cgccagggta
     6421 acctgtatgc gggcgatacc cagaacacca actctgacgc ttacactcgc tcgaaatatg
     6481 gcgatgaaac caaccgcctg tatcgccaga actacgcgct gacctggaac ggtggctggg
     6541 ataacggcgt gaccaccagc aactgggtgc agtatgaaca cacccgtaac tcgcgtattc
     6601 cggaaggtct ggcgggcggt accgaaggga aatttaacga aaaagcgaca caggatttcg
     6661 tcgatatcga tctcgatgac gtgatgctgc acagcgaagt taacctgccg attgatttcc
     6721 tcgttaacca gacgctgacg ctgggtacgg agtggaatca gcaacggatg aaggacttga
     6781 gctccaatac ccaggcgctg accggggcga acaccggcgg cgctattgat ggtgtgagtg
     6841 ccaccgaccg tagcccgtat tcaaaagcag aaattttctc gctgtttgcc gaaaacaata
     6901 tggagctgac tgacagcacc atcgtaacgc cggggctgcg tttcgatcat cacagtattg
     6961 tcggcaataa ctggagcccg gcgctgaaca tatcgcaagg tttaggcgat gacttcacgc
     7021 tcaaaatggg cattgcccgt gcttataaag caccgagcct gtaccagact aacccgaact
     7081 acattctcta cagtaaaggt cagagctgct atgccagcgc gggcggctgc tatctgcaag
     7141 gtaacgatga cctgaaagca gaaaccagca tcaacaaaga gattggtctg gagttcaaac
     7201 gcgacggctg gctggcgggc gtcacctggt tccgtaacga ttatcgcaat aagattgaag
     7261 cgggctatgt ggctgtaggg caaaacgcag tcggcaccga tctctatcag tgggataacg
     7321 tgccgaaagc agtggttgaa ggtctggaag gatcgttaaa cgtaccagtt agcgaaacgg
     7381 tgatgtggac caataacatc acttatatgc tgaagagtga aaacaaaacc acgggcgacc
     7441 gtttgtcgat catcccggag tacacgttga actcaacgct gagctggcag gcgcgggaag
     7501 atttgtcgat gcaaacgacc ttcacctggt acggtaagca gcagccgaaa aaatacaact
     7561 acaaaggtca gccagcggtt ggcccggaaa ccaaagaaat cagtccttac agcattgtgg
     7621 gtctgagcgc gacctgggat gtgacgaaga atgtcagtct gaccggcggc gtggacaacc
     7681 tgttcgacaa acgtttgtgg cgtgcgggta atgcccagac cacgggcgat ctggcagggg
     7741 ccaactatat cgcgggtgcc ggggcgtata cctataacga gccgggacgt acgtggtaca
     7801 tgagcgtaaa cactcacttc tgatgctaac gtcagattgt tgacaaagtg cgcgtcgttc
     7861 atgccggatg cggcgtgaac gccttatccg gcctacaaaa atcttgccaa ttcaatatat
     7921 tgcaggacca tgtaggcctg ataagcgtag cgcatcaggc agtttagcgt ttgtcatcag
     7981 tctctaatat ggtcgacatg aaaactacgc atacctccct cccctttgcc ggacatacgc
     8041 tgcattttgt tgagttcgat ccggcgaatt tttgtgagca ggatttactc tggctgccgc
     8101 attacgcgca actgcaacac gctggacgga agcgcaaagc cgaacattta gccgggcgga
     8161 tcgctgcaat ttatgcgctg cgggaatatg gctataaatg tgtgcccgca atcggcgagc
     8221 tacgccaacc ggtgtggcct gcggaggtat acggcagtat tagccactgc ggggctacgg
     8281 cattggccgt ggtttcccgt caaccgattg gcgttgatat cgaagaaatt ttttctgcgc
     8341 aaacagcgac cgaattgaca gacaacatta ttacaccagc agaacacgag cgactcgcag
     8401 actgcggttt agccttttct ctggcgctga cactggcatt ttccgccaaa gagagcgcat
     8461 ttaaggcaag tgagatccaa actgatgcag gttttctgga ctatcagata attagctgga
     8521 ataaacagca ggtcatcatt catcgtgaga atgagatgtt tgctgtgcac tggcagataa
     8581 aagaaaaaat agtcataact ctgtgccaac acgattaatt gacaacatct ggtacgattc
     8641 gcccgcagcc atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac cactctgacc
     8701 tgacaaccgg atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc
     8761 gagtacagcc ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgagtctcc
     8821 gaccagaagc gtaaatccca gtaccgtcaa acacagcact atgactgcca caagggcata
     8881 tttcgtcagc atgtttatat tgcctccatg cgaagaaggg actaccatcc aaattgttca
     8941 ggtttgggta ggtagcccca gggttgatag aaatatcgtc ctggggcttc atctttccag
     9001 acctcagagt aaacctgaaa ccggaaagct tcaggcaccc gcagttatct tacccttgct
     9061 tagcaaaata gctgtttatt tatacagcaa gtcctgtttc ggacttctca ctccctattt
     9121 gatcaactga agctgaaccc gtttttttct gcatgcctca atcgtcaaaa ttcttcgcca
     9181 ccagccagcc gtgccataac ttgtcacatt gcaaaagtag ccaaattgct tcagtcacca
     9241 tcaccaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc cgacttctat
     9301 actgaataga aaacgccaac ttaagagaaa cctatgccat tacccgattt tcatgtttct
     9361 gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc gggctatgac
     9421 ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac ggccggagag
     9481 gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg ccgtgatatc
     9541 aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg tattgcaggc agccgcagac
     9601 catcatctgg aaatttgcgg cggtggcaca cacccgtttc agaaatggca gcgacaggag
     9661 gtatgcgata acgagcgcta tcaacgaacg ctggaaaact ttggttatct cattcaacag
     9721 gcgaccgttt ttggtcagca tgtccatgtt ggctgcgcca gtggcgatga cgccatttat
     9781 ttgctgcacg gcctgtcacg gtttgtgccg cacttcatcg ccctttctgc cgcgtcgccg
     9841 tatatgcagg gaacggatac gcgttttacc tcctcacgac cgaatatttt ttccgccttt
     9901 cctgataatg gcccgatgcc gtgggtcagt aactggcaac aatttgaagc cctgtttcgc
     9961 tgtctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga tattcgcccc
    10021 agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac ccttagccac
    10081 gcggtaaata tggcgggatt aattcaggct accgcccact ggttactgac agaacgcccg
    10141 ttcaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca ggcctgtcgc
    10201 tatgggctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc gctaacggaa
    10261 gacaccttgc gattgctgga aaaaatcgcc ccttccgcac ataaaattgg tgcatcgagc
    10321 gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga atgaagcgca gctgatgcgc
    10381 gatttcgtcg ccgatggtgg ctcgctgatt gggctggtga aaaagcattg tgagatctgg
    10441 gccggtgact aagtagggaa ttgccttaca gaagaatatc cctgacaatg gtgttttaac
    10501 ttacatttaa caatgttatg aaacaccctt tagaaacctt gactaccgca gcaggcattt
    10561 tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg ctgacgctgg
    10621 cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac actttattgt
    10681 tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt tgttctgcgc tttatctggc
    10741 gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgtacttcgc ggcatacttt
    10801 gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca cgaaacggcg
    10861 aaacgcctgg cccttgagtt accctttgtc gagctttgct ggccttttgg cccggagttc
    10921 gatgttttta aaattggcgg caaaattttt atgctgtcgt cggagctgcg cggcgtcccc
    10981 tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcagat atacccgagc
    11041 attaagccag ggtatcacat gaataaaaag cactggattt cggtgtatcc cggcgaggaa
    11101 atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt ggttgatggt
    11161 ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcggaaatct atagcgcatt
    11221 tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa attactttca
    11281 catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac taaggcattt
    11341 gatgccagta aaaaacttac cccggaacag gccgagcaga tcaaaactct cctgcaatac
    11401 agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac ggaagaaggt
    11461 aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacgaacg caaaatactt
    11521 gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc ctggctgaag
    11581 ctggttgttg accaggaaga tgctgatggc cgctttgcca cgccggaagc gaaagccgcg
    11641 aacgataaag gtcgcaagtt cttcgctgat atgcaccgta aagatctgca tgatgatgca
    11701 gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg cgtggcggcg
    11761 ctgggtctgg acgcggtacc catcgaaggt tttgacgccg ccatcctcga tgcagaattt
    11821 ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca tcacagcgtt
    11881 gaagatttta acgctacgct gccgaaatca cgtctgccgc aaaacatcac cttaaccgaa
    11941 gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc tcctgatttg
    12001 cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac gttatgcagg
    12061 atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc acctcgatgg
    12121 tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt ttgcattggg
    12181 tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg ttgcaaatca
    12241 ttacccagaa taaactcttc caccgtttag cttttaccct gcaggggatt atcgtcaata
    12301 ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg actacctgcg
    12361 cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg gatgttattt
    12421 tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg atatttcagg
    12481 ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg ctgattggtc
    12541 agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg atgttggttt
    12601 ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac gatatgctga
    12661 aactgggcga ctggctggag atgccgaaat acggcgcgga tggcgcggtg atcgatattg
    12721 ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt cccacctggt
    12781 ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc gggcgacgta
    12841 ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa gacgaaatgc
    12901 aacgtctgaa taaagcgcac ttgttaaaac cttatttaac cagccgccat caggaaatta
    12961 atgagtggaa tcgccagcaa ggttctacgg agtcgatatt aaacctgcgc cgaatgacca
    13021 atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg cggattcgta
    13081 aagatatgac tttaatggta cgccaactgg ctccaggtga taacggttta ccgctcgaga
    13141 tctatgcgtt taccaacacc gtggtgtggc tggaatatga aagcattcag gctgatatat
    13201 tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag tcgccaaccg
    13261 gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa ccgctctcat
    13321 cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt atttccgacg
    13381 cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga tcgctgacaa
    13441 gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg caatgcaaag
    13501 atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct gacgtcgcat
    13561 cgctgcacga aaacgcagat gcgccaggca gatcataatc cagttcaaca gcagcgttgc
    13621 taccaccagc gccatcagca gaccaaacgc tttttgcggc agcagatagt tgattaatac
    13681 caccagcgaa gtgatcgctc cggaaagcat cagcgaatta atcggcacac cgcgacggct
    13741 gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca gcatgcggct
    13801 gttagagtaa accccgctgt tatacactga cagcgatgct accagaatga cgaagttcag
    13861 cgcagaagct accacgttgc tgtcgagatt atggaaaatc atcacaaacg ggctactgtt
    13921 ggatttcact tccacccacg gatagagcgc cagtaaaacc accagtgagc cgatgtaaaa
    13981 cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt ccggatcgcg
    14041 cgcttcagcg gcagtaatcc caatcagctc cagaccgccg aaggagaaca taattaccgc
    14101 cagcgacaaa atcagcccat tccagccggt ggcaaagaaa ccaccgtagc gccagaggtt
    14161 gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc caaagccgat
    14221 cataccgatg atcgccagca ctttaatcaa cgcaaaccag aactcggttt cgccatataa
    14281 gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag cccaaatcca
    14341 cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca gctctgccat
    14401 ccccaccagc acgaacatca cccagtagtt ccagccagag aggaagcccg cgaacggtcc
    14461 ccagtattta taggcaaagt gggcaaacga accggatacc ggctcttcga ccaccatctc
    14521 gccgagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc ccagcaatac
    14581 agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag taccaattgc
    14641 gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa gcgtcggctc
    14701 ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt tcccctgtgt
    14761 gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa gacacaatcc
    14821 acacggttaa acggggtatc ctgcttttat ttccgtaccc gatgtcggtg cagccacatc
    14881 agcttatacg ccgccgggat aataaacagc gacagcaaag gtgcggtgat catgccgcca
    14941 atcatcggtg cggcaatccg gctcatcacc tctgaccctg ctcctgttcc ccacagaatc
    15001 ggcagcagac cagcgataat caccgccacc gtcatcgctt tcgggcgcac gcgcagtacc
    15061 gcgccgtgat acaacgcctc atccagcttc tgctcgctga atgtttgcgg attattcaac
    15121 gacggctcgg cctctatggc gtgacgtaaa tacatcagca tcaccacgcc aaattcggcg
    15181 gcgaccccgg cgagggcgat aaagccagtg cccgtcgcca cggaaagatg aaagcccatc
    15241 caccacagca accagatgcc gcccaccagc gcaaacggta cgctgctgat aatcagcaac
    15301 gcttcgccca cccgacggaa cgccagatac aacagcacga agataatcat caacgtcatc
    15361 ggcaccatga gtttaagctt atggttggcg cgctccagta gctcgaactg cccggagaat
    15421 gccacgctgg tgcccggttt taactgcact ttttcagcta tcgctttttg caaatcgtga
    15481 accaccgaca ccatgtcacg atcgcgggca tcgatataaa tccagctcgt cgggcgcgca
    15541 ttctcggttt tcagcattga cggtccggta gagactttaa tgtcggccac gtctgccagg
    15601 gtgatttgct gcttcatcgg cgtcagaatc ggcagctggc gcagtgcctg cggactatcg
    15661 cgccagcttt gcggataacg cagattaatt ggataacggg caattccttc caccgtttcg
    15721 ccaaccatcg ccccgcccac agcagaagtc acaaacaact gcacatccgc caccgtcata
    15781 ccgtaacgcg cggctttttc acggttaatc tcaacgttga tatagcgccc accttccagc
    15841 cgctcggcaa gagcagaagc tacgcctggc accgttcgcg ccacttcttc aatttgctca
    15901 gccatcgcgt cgatatccgc cagcacagtg ccggaaactt taatgccgat ggggctttta
    15961 atgccggttg agagcatatc gatacggtta cgaattggcg gcacccacag attcgccagc
    16021 cccggcagcc gcacggtgtt atccagttcc tcaatgattt tgtccatcgt catgcctggc
    16081 cgccactgct cctgcggctt aagctggatg gtcgtttcta ccatctccag cggagcagaa
    16141 tcggtggcgg tttccgcttt cccggttttg ccaaataccc gcgccacttc aggtacgctc
    16201 ataattagct tgtcggtttt ttgcagcata ctcgccgcct ctgctgcgga aatccccggc
    16261 agcgtcgatg gcatatacaa caagtcgcct tcattgatct gcggtaaaaa ttccccgcca
    16321 actttattga gcggccagag aaccgtcagc accgaaagcg ccgccaccag cagcgtggtt
    16381 ttcggccagt gcagtacttt cagcaacagc ggatgataaa cacgaatcaa aaagcgattg
    16441 agcgggttac tgctttccgg cggaattttg ccacggatcc agtagcccat caggatcggg
    16501 atcactacga tcgccagcag cgccgcaccc gccatcgcat acgttttggt gaacgccaac
    16561 ggtccaaaca gacgcccttc ctgcccttcc agggtgaaga tcgggataaa cgacaacgtg
    16621 ataatcagca gactgataaa tagcgccggc cccacttcaa cagacgcatc ggtgatcacc
    16681 tgccagcgcg ttttattatc cagcgtggcg tcaggatgct ggtgctgcca ctcttccagc
    16741 cgtttatgtg cattttcgat catgacgata gcggcatcga ccatcgcccc gacggcaatc
    16801 gcaatgccac ccaacgacat aatattggca ttcagtcctt ggaagtgcat gacaataaaa
    16861 gcaatacaca accccagcgg caacgaaata atcgccacca gcgccgagcg cacatgccag
    16921 agaaacagcg cacagactac cgccaccaca ataaactctt ccagcaactt gccgctgagg
    16981 ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg ttgtaactat ctccacgcct
    17041 tccggcagac tacttttcag cgtttccagt ttgtccttca cggcggcgat cacttctcgg
    17101 gcgtttttgc cggatcgcag gatcaccacc ccgcccgcca cttcgccttc gccgtttagt
    17161 tcggcaatgc cccggcgcat ctccgggcca atctggacct tcgcaacatc gcgcagataa
    17221 acgggcacgc cattttcact ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgc
    17281 agatagccgc tggcgcgcac catatattcc gcttccgcca gttcgatcga cgaaccgccc
    17341 gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg cgagactgat gccatactgc
    17401 gccaggcgct ggggatcgat aaccacctga tactctttca ccacaccgcc caccgacgcc
    17461 acttccgcaa cgtcagggat ggttttcagc tcatatttga gaaaccagtc ctgtaatgag
    17521 cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca gtgcatattc atagatccag
    17581 ccaacacccg tggcatctgg tcccagctcg gcgctgactc ccgcaggcag cttaccctgt
    17641 acctggttga ggtactccag cacccgcgaa cgcgcccagt acggatcggt gccatcttcg
    17701 aaaatgacat acacataaga gtcgccaaac tgtgagaaac cgcgcacagt cttcgcgcca
    17761 ggcaccgaca acatggtggt ggttagcgga taagtcacct gattttcaac gatttgtggt
    17821 gcctgaccgg gatagctggt tttaataata acctgcacat cggagagatc cggcagcgca
    17881 tccactggcg tattaatgat ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc
    17941 agcaccaaaa aacggttcgc caccgagcga cgaataatcc attcaatcat tggttattcc
    18001 ctcaatgcgc atgggtagca ctttcagagc gcatccgctc cagtgcgcca gaaatattgg
    18061 cttcggaatc aatcaggaac aggccgctgg aaaccacctt ttcaccttcc gccagaccag
    18121 agcgtaatgc ggtgacgcct tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc
    18181 gcccgtcggc atcaacggta atcacccgct gttcgctgcc ggtatcaatc agcgcttgtg
    18241 acggaatgag cagcatcggt tcgctggcgg tgttgagttg caaccaggcg ttcatgcccg
    18301 gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag ctgcagcgtg cgggtcgcgg
    18361 catccacgcc aggtagcagc gtccatttgc ggatggtgag tgttttatcc ggtcgcgccg
    18421 gaacggtgag ggtaaactgc gaggcatctt tcaccagcca ggcgatagac tccgggatcg
    18481 cagcagtgac ccacaccggg tccataccct gaattttcgc taccacgtta tctttggaga
    18541 tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg
    18601 taaagcgagt ctggattttt tgcgtggcga tcaggcggcg aatatccgcc tccggcattc
    18661 ccgccagtcg cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc
    18721 gcagcagtaa atactcactc tgcgcttcca cccagtcagg aatggtcagg tcgagaagcg
    18781 gtgtgccctt ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg
    18841 cagcgcgagc ctgcacaatg gcatactgat actcgttgta actgacattc gccgggaaac
    18901 tctgggcaaa agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg
    18961 tctgagtcgg gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca
    19021 ccagatccat atccataaac ggcgatttac ctggtttatc gaaccgcgta ttggggtaca
    19081 tcgggtcgta ccagaataag attttacgtt ctgcggtcga cgttttttct gcgggtggtt
    19141 cagcctttgc aaaccaggta aaacctgccg cagaaataat accgcccgcg atcatgctgc
    19201 cgataataag cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat
    19261 cctgtaataa agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt
    19321 taatttcact cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt
    19381 tcacagcagc aatcggatcg tgatggatgg tgattttttt gctttccaga tcaataccct
    19441 ttacctcgcc agtggcacta ataacctgtg gttgtgcttc gctcatggtt tcatgatgat
    19501 gttcgttagc ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca
    19561 gtgctttttt catttattct ctcctggagt taaaagttat tgctgctaac cgccaccaag
    19621 tgcggtatat aaagaaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg
    19681 tcgggttgca aataacgaac gttcggcatc cagcacttcc agataactta ctgcgccgtg
    19741 ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gatgccagat aacgctgctg
    19801 ggcgctaatt tgatcgttca ggctttgacg taatgccagc gcatctgcca cttctttaaa
    19861 ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa tttcggcgat
    19921 atccagattg gcctggttgc gtcc
//
LOCUS       DAZFVO020000093        19685 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFVO020000093 DAZFVO020000000
VERSION     DAZFVO020000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.093"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..547)
                     /locus_tag="ACHKNB_RS15395"
                     /old_locus_tag="pgaptmp_003079"
     CDS             complement(<1..547)
                     /locus_tag="ACHKNB_RS15395"
                     /old_locus_tag="pgaptmp_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4196544.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYN"
     gene            complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHKNB_RS15400"
                     /old_locus_tag="pgaptmp_003080"
     CDS             complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHKNB_RS15400"
                     /old_locus_tag="pgaptmp_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HGU4196545.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHKNB_RS15405"
                     /old_locus_tag="pgaptmp_003081"
     CDS             complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHKNB_RS15405"
                     /old_locus_tag="pgaptmp_003081"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HGU4196546.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHKNB_RS15410"
                     /old_locus_tag="pgaptmp_003082"
     CDS             2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHKNB_RS15410"
                     /old_locus_tag="pgaptmp_003082"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HGU4196547.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(4227..4409)
                     /locus_tag="ACHKNB_RS15415"
                     /old_locus_tag="pgaptmp_003083"
     CDS             complement(4227..4409)
                     /locus_tag="ACHKNB_RS15415"
                     /old_locus_tag="pgaptmp_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HGU4196548.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDY"
     gene            complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHKNB_RS15420"
                     /old_locus_tag="pgaptmp_003084"
     CDS             complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHKNB_RS15420"
                     /old_locus_tag="pgaptmp_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HGU4196549.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHKNB_RS15425"
                     /old_locus_tag="pgaptmp_003085"
     CDS             complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHKNB_RS15425"
                     /old_locus_tag="pgaptmp_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HGU4196550.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHKNB_RS15430"
                     /old_locus_tag="pgaptmp_003086"
     CDS             complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHKNB_RS15430"
                     /old_locus_tag="pgaptmp_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HGU4196551.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHKNB_RS15435"
                     /old_locus_tag="pgaptmp_003087"
     CDS             complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHKNB_RS15435"
                     /old_locus_tag="pgaptmp_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4196552.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHKNB_RS15440"
                     /old_locus_tag="pgaptmp_003088"
     CDS             complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHKNB_RS15440"
                     /old_locus_tag="pgaptmp_003088"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4196553.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHKNB_RS15445"
                     /old_locus_tag="pgaptmp_003089"
     CDS             complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHKNB_RS15445"
                     /old_locus_tag="pgaptmp_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HGU4196554.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHKNB_RS15450"
                     /old_locus_tag="pgaptmp_003090"
     CDS             complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHKNB_RS15450"
                     /old_locus_tag="pgaptmp_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HGU4196555.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9952..10002
                     /gene="hisL"
                     /locus_tag="ACHKNB_RS15455"
                     /old_locus_tag="pgaptmp_003091"
     CDS             9952..10002
                     /gene="hisL"
                     /locus_tag="ACHKNB_RS15455"
                     /old_locus_tag="pgaptmp_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HGU4196556.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    9978..10101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            10148..11047
                     /gene="hisG"
                     /locus_tag="ACHKNB_RS15460"
                     /old_locus_tag="pgaptmp_003092"
     CDS             10148..11047
                     /gene="hisG"
                     /locus_tag="ACHKNB_RS15460"
                     /old_locus_tag="pgaptmp_003092"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HGU4196557.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            11053..12357
                     /gene="hisD"
                     /locus_tag="ACHKNB_RS15465"
                     /old_locus_tag="pgaptmp_003093"
     CDS             11053..12357
                     /gene="hisD"
                     /locus_tag="ACHKNB_RS15465"
                     /old_locus_tag="pgaptmp_003093"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HGU4196558.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILCAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRQIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            12354..13424
                     /gene="hisC"
                     /locus_tag="ACHKNB_RS15470"
                     /old_locus_tag="pgaptmp_003094"
     CDS             12354..13424
                     /gene="hisC"
                     /locus_tag="ACHKNB_RS15470"
                     /old_locus_tag="pgaptmp_003094"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HGU4196559.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            13424..14491
                     /gene="hisB"
                     /locus_tag="ACHKNB_RS15475"
                     /old_locus_tag="pgaptmp_003095"
     CDS             13424..14491
                     /gene="hisB"
                     /locus_tag="ACHKNB_RS15475"
                     /old_locus_tag="pgaptmp_003095"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HGU4196560.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELMKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            14491..15081
                     /gene="hisH"
                     /locus_tag="ACHKNB_RS15480"
                     /old_locus_tag="pgaptmp_003096"
     CDS             14491..15081
                     /gene="hisH"
                     /locus_tag="ACHKNB_RS15480"
                     /old_locus_tag="pgaptmp_003096"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HGU4196561.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVHKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            15081..15818
                     /gene="hisA"
                     /locus_tag="ACHKNB_RS15485"
                     /old_locus_tag="pgaptmp_003097"
     CDS             15081..15818
                     /gene="hisA"
                     /locus_tag="ACHKNB_RS15485"
                     /old_locus_tag="pgaptmp_003097"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HGU4196562.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            15800..16576
                     /gene="hisF"
                     /locus_tag="ACHKNB_RS15490"
                     /old_locus_tag="pgaptmp_003098"
     CDS             15800..16576
                     /gene="hisF"
                     /locus_tag="ACHKNB_RS15490"
                     /old_locus_tag="pgaptmp_003098"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HGU4196563.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITHLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHKNB_RS15495"
                     /old_locus_tag="pgaptmp_003099"
     CDS             16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHKNB_RS15495"
                     /old_locus_tag="pgaptmp_003099"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HGU4196564.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHKNB_RS15500"
                     /old_locus_tag="pgaptmp_003100"
     CDS             complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHKNB_RS15500"
                     /old_locus_tag="pgaptmp_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HGU4196565.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHKNB_RS15505"
                     /old_locus_tag="pgaptmp_003101"
     CDS             complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHKNB_RS15505"
                     /old_locus_tag="pgaptmp_003101"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGU4196566.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         5072 a         5132 c         4843 g         4638 t
ORIGIN      
        1 gattatactc ggtcagactt ttcgccagtt gaatgcgcca gtggatgaac gcccgttgtg
       61 gccagatacc ggtaaacaac attgccagta aagagccgag gatcacatcg ccgcttcgcc
      121 ataacgccgt atcaatttca cctgtcgggg aaccaacaac aattgccagc gtcaccccaa
      181 tcaataaacc ttgatacggt ttcttgccca gcgccagcca accgcaaaga aacatcgccg
      241 ccgcgcacca gactaacatc agcggtaacg aaattaactc cagttgcaga gcgataagac
      301 ctaaaatcga acccaacacc gtaccgccaa tacgttcgaa ggcgcgaggg acaacgttac
      361 cccagaacga gattggcccc ataatcacca ccatggtgac cagcggccag gtgctttccg
      421 ggatagtaaa caggcggata atgagaaaag tgagcaggaa tgccagcgcg acccgagtac
      481 catgcacaat gcggtaatgg cggtataccc ggatttcaaa cgggcttaat gacttatcgg
      541 cacgcacgca gaaactccgt aatgaagatt aaaagcgctc ataactcatc tgtcggccag
      601 aaggcgcacc tctgatttat ccgacggaca taccgggaac agtgtaattt cccggcagga
      661 gcgaaaaatt tgaaaggcgc atcttactct tttcgctttc aaaaaaagag tggtcttcgc
      721 gtcaacacac cgccctgaga tgaattagtg atgttttggc tgcaccgcaa catacatttc
      781 gatatcccag tacccatctt ccgcgccatt gttcaagtaa acctcgaagc agggctttgg
      841 taacatttca taagcactgt cctgcaagag actattaaag aactgatacc agggtttagc
      901 aaaatcatca ccgactacac gagctaccgc caccgcatac tgaccacctg taatttctgt
      961 cagaatgacg ccctcactgt tttcgggaag cgtaaagtaa cccggcaccg tcacgacggt
     1021 gtcgcagcgt aatttttcgg cgggtgtttc atctggattg tcgtaataga cagcaaccca
     1081 ctccttcggc acaatatttt tgttatctac ccacatcatc aactgctcaa agcctttctt
     1141 taccgtctgt tcccacgggc caacgagatg gaaacctgca acggtacgtt tctcttcctg
     1201 cttaatctcg tagttcatga cgcctccatt aacaccgttt ttatatacag tatagttgca
     1261 atattaaaac cacaaggaat gagtgttgat tatgcgagca gactcgcact cctgccagtc
     1321 tgctgcaaaa gaaaggtcag gccttatggt ggaaataatc actcaggcgg gaaaacatgc
     1381 cgccttcccc gacagattcc agggtaacca gcggccagtg cgccacctgt ttatcacggt
     1441 cgtaaagttc aatttcccct acccgctgat gggcgctaat tggcgcggtg agctctttac
     1501 catcaagggt atatttggct ttgatatgtg gaatttcggc tttcggtagc accatccaga
     1561 actcttgttc cgttcccagg tcgatatttt ctttatcgcc ataccagatg cgttccgttc
     1621 cgaccttttt cccacggtgc aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca
     1681 gtaattttct tgcctcttcc tcacgacctt ttgcactgtc agcccccatt accactgcaa
     1741 tgagacgacg ctgcccatct acagccgaag caatgagatt aaacccggca ccagaagtat
     1801 gacccgtttt caggccgtca acattcatgg ttttatccca caacaacccg ttacggtttt
     1861 gctgggtgat accgttccag gtgagacttt tctcactgta catatgataa aactcgggct
     1921 cgccgtggat gatagcgcga gaaagcacag ctaaatcata agccgagcta tgctggccag
     1981 gtgcatccag accatgcact gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat
     2041 agttgttcat catttcaaca aactgccgtt gcccaccggc aatatagtca gccagagcaa
     2101 cacaagcgtc atttccggaa tccacaatta aaccacggct taaatcacgt accgatacgc
     2161 gatcgccctc tttcaaaaac atcagtgaag aaccgacaaa caccggatta tctttcgccc
     2221 acgcatcgcg ccccacggtg acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc
     2281 gatccacgac ataacccgtc atcagctttg tcaggctggc gggattgcgc tgttgatgct
     2341 cattacccgc ggtgaggatc tgaccggtgg tgtaatccat caatacccag gacccggcat
     2401 gaatctctgg aggctgaggt gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt
     2461 taaaaacgaa caaagaagca gcaataataa gacggcgttt caacagcaaa ccctcaggag
     2521 tttcaaatag ctgttctttt tacggaaata cttatgaact ggctggaata aagtgcaaga
     2581 aaatgtgact accctctcat ttttatctga catgatctgt tgccactcgc tgccaaattg
     2641 tggcgctaaa gctgattagc acggtgatat ttgatactct ggcagacagc agaaataacg
     2701 gatttaacct aatgatgaat gacggtaagc aacaatctac ctttttgttt cacgattacg
     2761 aaacctttgg cacgcacccc gcgttagatc gccctgcaca gttcgcagcc attcgcaccg
     2821 atagcgaatt caatgtcatc ggcgaacccg aagtctttta ctgcaagccc gcggatgact
     2881 atttacccca gcctggagca gtattaatta ccggtattac cccgcaggaa gcacgggcga
     2941 aaggagaaaa cgaagccgcg tttgccgccc gtattcactc gctttttacc gtaccgaaga
     3001 cctgtattct gggctacaac aatgtgcgtt tcgacgacga agtcacacgc aacgtttttt
     3061 atcgtaattt ctacgatcct tacgcctgga gctggcagca tgataactcg cgctgggatt
     3121 tgttggatgt tatgcgtgcc tgttatgccc tgcgcccgga aggaataaac tggcctgaaa
     3181 atgatgacgg tctaccgagc tttcgccttg agcatttaac caaagcgaat ggtattgaac
     3241 atagcaacgc ccacgatgcg atggctgatg tgtacgccac tattgcgatg gcaaagctgg
     3301 taaaaacgcg tcagccacgc ctgtttgatt atctctttac ccatcgtaat aaacacaaac
     3361 tgatggcgtt gattgatgtt ccgcagatga aacccctggt gcacgtttcc ggaatgtttg
     3421 gagcatggcg cggcaatacc agctgggtgg caccgctggc gtggcatcct gaaaatcgca
     3481 atgccgtaat tatggtggat ttggcaggag acatttcgcc attactggaa ctggatagcg
     3541 acacattgcg cgagcgttta tataccgcaa aaaccgatct tggcgataac gccgccgttc
     3601 cggttaagct ggtgcatatc aataaatgtc cggtgctggc ccaggcgaat acgctacgcc
     3661 cggaagatgc cgaccgactg ggaattaatc gtcagcattg cctcgataac ctgaaaattc
     3721 tgcgtgaaaa tccgcaagtg cgcgaaaaag tggtggcgat attcgcggaa gccgaaccgt
     3781 ttacgccttc agataacgtg gatgcacagc tttataacgg ctttttcagt gacgccgatc
     3841 gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa tttaccggca ctggatatca
     3901 cttttgtcga taaacggatt gaaaagctgt tgttcaatta tcgggcgcgc aacttcccgg
     3961 ggacgctgga ttatgccgag cagcaacgct ggctggagca ccgccgccag gtcttcacgc
     4021 cagagttttt acagggttat gcagatgaat tgcagatgct ggcacaacaa tatgccgatg
     4081 acaaagagaa agtggcgctg ttaaaagcgc tttggcagta cgcggaagag atcgtctaat
     4141 ttgaagcctc cgccgctggt acggcggagg acaatgttga gtcaggcttt ttgaacggtg
     4201 atgctccacg ctgcatcgcc aatctgctaa taatcggtga tggcatgtcc ttcttctgcc
     4261 gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa actcaattac caactcatcg
     4321 ccgctggcca tttctgccag tgctgctttt gcttctatga gtggaaacgg acagacctgc
     4381 gtcaccacat ccagcttttt aatcgccatc tttacacctt acttaatttg ccgcagcagt
     4441 tgccagtcgc gccttacgct gcggtcgaac atacacaagc caggacgcag tccagactcc
     4501 gagaatcata aatacaaggc caatccagcc ctgccaggtc atcattgcag tcataaccag
     4561 tccattaccg atagagcaac cacctgcaat actggcaccg aagcccatca gtacaccacc
     4621 taacccgcta cgtaatgttg tttgtgcatc agctgcgcga acgcggaact cacggctcgc
     4681 tttggctgca ataaatgacc ccacgaagat ccctaacacc aggaaaacgc cccagttaat
     4741 gtatttcacg tcacccgcga ccagaaattg cagaatattg gccgttgggg aagtgattcc
     4801 taacccaaac atgcgtccgg ttgcttcact caggggccag gctaaaagcg caatcaaacc
     4861 gatgagtaca gcagtgacaa agggatgcca gcgtttttca aacagaatat gagcgatccc
     4921 ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc ttcttcaact ctttcatcac
     4981 cacccagagc gttatcacca gcaaaacggc aaccaacggc cacacagaca aattaaaagt
     5041 ctcagcaata gagttatgtt cagtactgta gtgctgcaag gtttgattta aaccactggc
     5101 atgtggagaa cgcatcaccg cactcatcac catataagtg aaaagcgcga tccaactgcc
     5161 gatcaatccc tcacccgcgc gataccaggt cccggtggca catccacccg ccagaacaat
     5221 tcccagcccg aagatatacc cacctataac agtaccaagc cacgggaacg ccccggcttc
     5281 gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc acgctttgta ccgaaattgc
     5341 aatcagcaag gcgtaaaaca tgcgattatt tttcacgata tacatatcgc gaaaaccacc
     5401 tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc agagcaccac aaattagccc
     5461 gcttaatatc attgaaaaca taaattaatt aaccagatga atgttaatag ggaaattatt
     5521 catgactggt ggaatgcaga ccaataacca aattctctaa attagaacaa atggttatta
     5581 atgaggatgt cgataaaaaa gaataaaaaa accggagcca aatattcatt cgactccggt
     5641 ttattattac aaaagatgat tacgctacgt cttcgtactg cggaaccggg ttacggaaac
     5701 tcttggtgac gcaagccagg taaatcagac cgatagccgc ccagatcaga cccagaacca
     5761 ttgagctttc ttccaggtta acccacagcg caccaacggt cagcgcacca cacatcggca
     5821 ggaacagata ctggaagtga tctttcagcg tcttattacg cttctcacgg atccagaact
     5881 gcgagatgac cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg
     5941 ctgtagccat taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga
     6001 tgttcatcgc cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaata
     6061 cgccgtcacg acccattacg tacatcagac gcgctacgcc tgcatgcgcc gccataccgg
     6121 acgccagtac ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa
     6181 cgtacagcat gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga
     6241 agtacagctg caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca
     6301 ggaaaatcgc acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga
     6361 tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa
     6421 catgcgcatc gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt
     6481 caaatacgcc ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga
     6541 cgattacagt gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcatga
     6601 aggccaccag cgccaccaca aacatccacg atgggatgga aggcactaga gcttcaaaat
     6661 agattttcgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc
     6721 aacccaccat aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc
     6781 cagcagaagg atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa
     6841 tcaatgcgaa cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa
     6901 aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta
     6961 acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg
     7021 tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat
     7081 tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct
     7141 cttggtatga atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt
     7201 tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga
     7261 gaggcttgtc ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata
     7321 aacaccggcg atcgcactat ttgctaaaat ttcatcccgc caccagccgc tggcaagccc
     7381 tgcggtctgt tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt
     7441 cttctcgatt aacgcgccac tatcgagaaa ccgttgcgcc agataacggg gtaaatagcc
     7501 gcagccgagg ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc
     7561 ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tatcccccac
     7621 cacaatcgcc cgatatcgct taataatacg acggtttaac ggctcttctt ccagagctaa
     7681 cggatgatga ggcgcgaccg caaagacttg ttccagatcg cccaaccgtg aaaaaccaaa
     7741 ctcactggaa gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt
     7801 cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg
     7861 atgttgataa aaagcttcaa tcagtggcgc aagcagagaa aaaggaaacg tgtcgtccac
     7921 gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc
     7981 tcgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg tacgggtgaa
     8041 tttagcgcgg tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg
     8101 aacggtgtaa ctcagagcgg acggcgtctt atagagtttg gccgacgccg ccgcaaaact
     8161 gccttctttt tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc
     8221 ccccttgcag tgtatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag
     8281 cggatcggga tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat
     8341 cttacctttg ccgctgtcca gactatttct gaactgcgga ggctccagcc ccagtaaacg
     8401 ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat
     8461 gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg
     8521 gactaaatta acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg
     8581 atgacgtccc ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa
     8641 attgtgcagc cagtcttcga gttcttctaa tactcgtcca ctgttggtta ccggattacg
     8701 cggggtagtt tctttcaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa
     8761 aataatgcgg ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa
     8821 atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc
     8881 atccatcagg gcatccagat cgtcagaatc gcaaactagc tcaggctcca tgcgaagcag
     8941 atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc
     9001 agtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa
     9061 cccgacaatt gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct
     9121 acgctagcgt atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac
     9181 gacatgctgc aatgagcagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg
     9241 taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag gggttctggc ttccccttac
     9301 cttcaaatgg cgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct
     9361 tatctgtttc ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca
     9421 ctcaataatg tccttttctg ttcctttgcc tgatttcagg ctatcgattg agtccatcaa
     9481 tctccgggcg ttagcggggg aacgcagtag ataagccgtc tcttccagcg agttgtattc
     9541 ttcgagtgac atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg
     9601 atcttcaacg gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat
     9661 tgtacgcatg tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa
     9721 ctatttatta attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg
     9781 ctatttttgg tgccgtcagc taagaggaca gtcctcttag ccccctcctt tccccgctca
     9841 ttcattaaac aaatccatcg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa
     9901 agtggtttag gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc
     9961 gttcaattta aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg
    10021 ctggaagaca ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc
    10081 accttccggg ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa
    10141 taacaaaatg acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag
    10201 tgatgactca cgtgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg
    10261 cctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat
    10321 tcccggtctg gtaatggacg gcgtggtaga tcttgggatt atcggcgaaa acgtgctgga
    10381 agaagagctg cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccttgcgtcg
    10441 tctggatttc ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg
    10501 cccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta
    10561 tctcgaccag aaaggcatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc
    10621 cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga
    10681 agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg
    10741 cgatggcgaa atggaagaat ccaaacagca actgatcgac aaactgctga cccgtattca
    10801 gggtgtgatc caggcacgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct
    10861 ggatgaagtc atcgccctgc tgccaggcgc cgaacgccca actattctgc cgctggcagg
    10921 tgatcaacag cgtgtagcga tgcacatggt cagtagcgaa accctgttct gggaaacgat
    10981 ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc ctgccgattg agaagatgat
    11041 ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa
    11101 caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt
    11161 aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg ccctgcggga atacagcgcg
    11221 aagtttgata aaaccacggt taccgcgctg aaggtgtctg ctgaggagat cgccgccgcc
    11281 agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa
    11341 accttccaca ctgcgcaaaa actgccgcca gtagatgtag aaactcagcc aggcgtacgt
    11401 tgtcagcaag tcacgcgccc ggtggcttcg gtaggtttgt atattcctgg cggctccgcc
    11461 ccgctcttct caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa
    11521 gtggtgctgt gctcaccgcc gccgattgcc gatgagatcc tttgtgcggc gcagctgtgc
    11581 ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt
    11641 acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa
    11701 gcgaaacgtc aggtgagcca gcgtctggac ggtgcggcta tcgatatgcc cgcaggcccg
    11761 tcggaagtgt tggtcattgc cgacagtggc gcaacgccgg atttcgtggc ttctgatttg
    11821 ctttctcagg ctgaacacgg cccggactca caggtgattt tactgacgcc cgatgctgat
    11881 atggcgcatc aagttgccga ggccgtcgaa cgccagttgg cagaactgcc gcgtgccgaa
    11941 accgcccgtc aggcacttag cgccagccgc cagatcgtga ctaaagattt agcgcagtgc
    12001 gtagagatct ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgt
    12061 gaactggtcg atagcattac cagtgccggt tcggtatttc ttggtgactg gtcaccggaa
    12121 tcggcaggtg attacgcctc cggcaccaac cacgttctac cgacttacgg ttacaccgcc
    12181 acctgttcca gcctcgggct ggcagatttc cagaagcgca tgaccgtgca ggaactgtcg
    12241 aaagaaggct tttctgcgct ggcttcgacc atagaaacac tggccgccgc cgagcgcctg
    12301 accgcccata aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca
    12361 ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg
    12421 cgcgtcgtct gggcggtaat ggcgacgtct ggctgaacgc caacgaatat cccacagccg
    12481 tggagtttca gcttactcag caaacgctca accgctaccc ggaatgtcag ccgaaagcag
    12541 tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg
    12601 gcgcggacga aggtattgaa ctactgattc gcgctttttg cgaaccgggt aaagacgcca
    12661 tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggcgtcg
    12721 agtgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca
    12781 agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga
    12841 tcaacccgca ggattttcgc accctgctgg agttaacgcg cggtaaggcg attgtggttg
    12901 ccgatgaagc ctatatagag ttttgtccac aggcatcgct ggctggctgg ctggcggaat
    12961 atccgcacct ggctattttg cgcacactgt cgaaagcttt tgctctggcg gggcttcgtt
    13021 gcggatttac gctggcaaac gaagaggtca tcaacctgtt aatgaaagtg atcgccccct
    13081 acccgctctc gacgccggtt gccgacattg cggcccaggc gttaagcccg caggggatcg
    13141 tcgccatgcg tgagcgggtg gcgcaaatta ttgcagaacg cgaatacctg attgccgcac
    13201 tgaaagagat cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc
    13261 gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg
    13321 atcagaataa acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag
    13381 aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct
    13441 ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg
    13501 ttttgataaa ctcgcctttg aaccgggcgt gatcccggag ctgatgaagc tgcaaaaagc
    13561 gggctacaag ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca
    13621 ggcggatttt gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca
    13681 gtttgatgaa gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc
    13741 gaaagtaaaa ctggtggagc gttaccttgc tgagcaagcg atggatcgtg ccaacagtta
    13801 tgtgattggc gatcgcgcga ccgatattca actcgctgaa aacatgggca ttactggttt
    13861 acgctacgac cgcgaaaccc tgaactggcc gatgattggt gagcaactca ctaaacgaga
    13921 ccgttacgcc catgtagtgc gcaacaccaa agagacgcag attgacgttc aggtgtggct
    13981 ggatcgcgaa ggtggcagca agattaacac cggtgttggc ttctttgatc acatgctgga
    14041 tcagatcgcc acccacggcg gtttccgtat ggaaatcaac gtcaaaggcg acctctatat
    14101 cgacgatcac cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc
    14161 cctcggcgat aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct
    14221 tgcccgctgt gcactggata tctctggtcg cccgcacctg gaatataaag ccgaatttac
    14281 ctaccagcgc gtgggcgatc tcagcaccga aatgatcgag cacttcttcc gttcgctctc
    14341 atacaccatg ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc accaccgtgt
    14401 agagagcctg ttcaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga
    14461 caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc
    14521 gccaatctga actcggtgaa gtcagccatt gcgcgccacg gttatgaacc aaaagtcagc
    14581 cgtgacccgg acgtcgtgtt gctggccgat aaactatttt tacccggcgt tggcactgcg
    14641 caagcagcga tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc
    14701 caaccggtac tgggcatctg cttagggatg caactgctgg gtcggcgcag cgaagagagc
    14761 aacggcgtcg acttgctggg catcattgac gaagacgtgc cgaaaatgac cgactttggt
    14821 ctgccactgc cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt
    14881 caggggattg aagacggcgc gtacttttac tttgttcaca gttacgcaat gccggtcaat
    14941 ccgtggacca ttgcccaatg taattacggc gaaccgttca ccgcggcggt acacaaagat
    15001 aacttctacg gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg
    15061 aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg
    15121 tgcgtctcca tcagggcgat tacggcaaac agcgcgatta cggtaacgac ccgctgccgc
    15181 gcttacagga ttacgccgcg cagggtgccg aagtgttgca tctggtggat ctgaccgggg
    15241 caaaagatcc ggctaaacgt caaatcccgc tgattaaaac cctggtggcg ggcgttaacg
    15301 ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg ttactggaag
    15361 cgggcgttgc gcgcgtggtg gtcggctcca ccgcggtgaa atcaccagaa atggtgaaag
    15421 gctggtttga acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg
    15481 agcaaggcaa caagcaggtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg
    15541 aacaactggt ggaaacctat ttgcccgtcg gcctgaaaca tgtgctgtgt accgatatct
    15601 cgcgcgacgg cacgctggca ggctctaacg tctctttata tgaagaagtg tgcgccagat
    15661 atccgcaggt ggcatttcag tcctccggcg gtattggcga cattgatgat gtcgctgccc
    15721 tgcgtggtac tggtgtacgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca
    15781 ccgtgaagga ggccatcgca tgctggcaaa acgcataatc ccatgtctcg acgttcgtga
    15841 tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc
    15901 gctggcaaaa cgctacgccg aggaaggtgc agacgaactg gtgttctacg atatcaccgc
    15961 ttccagcgat ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga
    16021 cattccgttt tgtgtggcgg gtgggattaa gtctctggat gacgccgcca aaattctttc
    16081 ctttggcgcg gataaaatct ccattaactc ccctgcgctt gcggacccga cgttaattac
    16141 tcacctggcg gatcgctttg gcgtgcagtg tattgtggta ggtattgata cctggtacga
    16201 cgctgaaacc ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt
    16261 cactcaatgg gaaacgctcg actgggtaca ggaagtgcaa aaacgcggtg ccggagaaat
    16321 cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacaactgaa
    16381 aaaagtgcgt gaagtttgcc acgtcccgct gattgcttcc ggtggcgcgg gcaccatgga
    16441 acacttcctc gaagccttcc gcgatgccga cgttgacggc gcgctggcag cttccgtatt
    16501 ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga
    16561 gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga
    16621 ctgatgccgg tgattgtgca acacgcggta tccggcgaag tgttaatgct gggctatatg
    16681 aacccggaag ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact
    16741 aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt tcctcaatgt ggtgagtatt
    16801 gccccggact gcgacaacga cacgttactg gtgctggcaa atccaatcgg gccaacctgc
    16861 cacaaaggca ccagcagctg cttcggcgac accgctcacc agtggctgtt cctgtatcaa
    16921 ctggaacaac tgctcgccga acgtaaatct gccgacccgg aaacctccta caccgccaaa
    16981 ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc
    17041 gcactggccg cgactgtaca tgaccgcttt gagctgacca acgaggcgtc tgatttgatg
    17101 tatcacctgt tggtattgtt gcaggatcag gatctggatt taacgacggt gattgagaac
    17161 ctgcgtaaac ggcatcagtg agttgcgggg taagcggatg cgatattgtt gtcgcatccg
    17221 gcaaaaaaaa acgggcaagg tgtcaccacc ctgcccattt tctttaaaac cgaaaagatt
    17281 acttcgcgtt gtaattacgc agagcgttac gccccaacac aatccccgcg ccaaccatgc
    17341 cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag
    17401 gtaacgtcgg tttcattaca tagcggtaag catgaatatc aagtttatca acgtcaagat
    17461 tgtcgatatc cagcaggttt tgacgagtct gatagtagtt tgatgagaac accaacggac
    17521 gagtcgcctc gtgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacatagtat
    17581 cctgggttac gtcctgtgtc tgctgaatct gcggctttgt cacctgcgcc tgattcgcat
    17641 actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcaaccactt
    17701 cctgggtctt cagagaatcc tgcaagtttt tcatttgcag cgcgatgttg tctttcaggt
    17761 cttgttccag ttcttcattt acctggtcgt caacttgctg gatatactgc gccagctgtt
    17821 tctgcgcacc ttcaggtgtt tcacccacat aagaaaccgc taaaggtaat gactgatttt
    17881 taacggtagg ctcaatggtc agtttttcag gttcttcctg attatccagc gtttctgcta
    17941 atgcagaaaa tgtcgtgctg aaacgaccaa ttaacatcga ctggatctca gacactttcg
    18001 gcgctgccga accataaatc acatttatgg cattgttata accagcgatt tgtcccacat
    18061 caggttgagt aacgatagct gtagaagtcc acttttcttt ggcaacggcc agatacccta
    18121 tagccagggc aatagcgaca atgacagaga tgataattgt catcttgcca cgccacaact
    18181 gcaccagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat
    18241 tattttctac tctcatcatt accctaacgg acgaaataat tttaagtcgc catagtttag
    18301 cgataatcgc catgaatgct ataggataaa tgataaaaat aatgaattat tacaaaggga
    18361 gcataagaga atcagggcga aaatcgccct gataacagga tgttagtcac tgccaaagag
    18421 atcgcgggtg tagactttgt ctgccacatc cttaagctct tctgccatac ggttggagat
    18481 aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccaggcgag agttgaagaa
    18541 tgagtcttct ttcatcactg gctcgtatat gatcacttca acgcctttcg ccttgatacg
    18601 tttcataatc ccctgaatgg aagacgcacg gaagttatct gatccgctct tcataatcag
    18661 acgataaata cccactactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt
    18721 acgtgtacgg tttgcatcga caattgccga aatcaggtta ttcggcacag actggtagtt
    18781 cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa acgacggatt
    18841 gttgtaatgg ttgccaattc gcggatcgag acaaacgcct tcgattattt gacgggaatt
    18901 cagacctaaa ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgccag
    18961 gtaggtgttt gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtagg
    19021 gatattttgc ttaatcgctc cttcctgtaa taacgcagcg aaacgttctg cgcgttctga
    19081 acgctcacca atgacaatac gtgaaggatg gagattatcg taaagggctt taccttcacg
    19141 gagaaattcc ggagagaaaa taaggttatc gatacccaga cgttctttaa ttgatttggt
    19201 gaaacctacc ggaattgtag atttaatcac catgacagca ttaggattaa tttctgtcac
    19261 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    19321 ggtaggcgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    19381 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    19441 tgactgcttc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    19501 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    19561 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    19621 cacaacttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    19681 tcctg
//
LOCUS       DAZFVO020000094        19642 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFVO020000094 DAZFVO020000000
VERSION     DAZFVO020000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.094"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(368..1165)
                     /gene="ybcM"
                     /locus_tag="ACHKNB_RS15510"
                     /old_locus_tag="pgaptmp_003102"
     CDS             complement(368..1165)
                     /gene="ybcM"
                     /locus_tag="ACHKNB_RS15510"
                     /old_locus_tag="pgaptmp_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4196567.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1175..1726)
                     /gene="ybcL"
                     /locus_tag="ACHKNB_RS15515"
                     /old_locus_tag="pgaptmp_003103"
     CDS             complement(1175..1726)
                     /gene="ybcL"
                     /locus_tag="ACHKNB_RS15515"
                     /old_locus_tag="pgaptmp_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HGU4196568.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1895..2227)
                     /gene="emrE"
                     /locus_tag="ACHKNB_RS15520"
                     /old_locus_tag="pgaptmp_003104"
     CDS             complement(1895..2227)
                     /gene="emrE"
                     /locus_tag="ACHKNB_RS15520"
                     /old_locus_tag="pgaptmp_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HGU4196569.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2561..2875)
                     /gene="fliE"
                     /locus_tag="ACHKNB_RS15525"
                     /old_locus_tag="pgaptmp_003105"
     CDS             complement(2561..2875)
                     /gene="fliE"
                     /locus_tag="ACHKNB_RS15525"
                     /old_locus_tag="pgaptmp_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HGU4196570.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3090..4748
                     /gene="fliF"
                     /locus_tag="ACHKNB_RS15530"
                     /old_locus_tag="pgaptmp_003106"
     CDS             3090..4748
                     /gene="fliF"
                     /locus_tag="ACHKNB_RS15530"
                     /old_locus_tag="pgaptmp_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HGU4196571.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4741..5736
                     /gene="fliG"
                     /locus_tag="ACHKNB_RS15535"
                     /old_locus_tag="pgaptmp_003107"
     CDS             4741..5736
                     /gene="fliG"
                     /locus_tag="ACHKNB_RS15535"
                     /old_locus_tag="pgaptmp_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HGU4196572.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVYDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5729..6415
                     /gene="fliH"
                     /locus_tag="ACHKNB_RS15540"
                     /old_locus_tag="pgaptmp_003108"
     CDS             5729..6415
                     /gene="fliH"
                     /locus_tag="ACHKNB_RS15540"
                     /old_locus_tag="pgaptmp_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HGU4196573.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6415..7788
                     /gene="fliI"
                     /locus_tag="ACHKNB_RS15545"
                     /old_locus_tag="pgaptmp_003109"
     CDS             6415..7788
                     /gene="fliI"
                     /locus_tag="ACHKNB_RS15545"
                     /old_locus_tag="pgaptmp_003109"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HGU4196574.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7807..8250
                     /gene="fliJ"
                     /locus_tag="ACHKNB_RS15550"
                     /old_locus_tag="pgaptmp_003110"
     CDS             7807..8250
                     /gene="fliJ"
                     /locus_tag="ACHKNB_RS15550"
                     /old_locus_tag="pgaptmp_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HGU4196575.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8247..9374
                     /gene="fliK"
                     /locus_tag="ACHKNB_RS15555"
                     /old_locus_tag="pgaptmp_003111"
     CDS             8247..9374
                     /gene="fliK"
                     /locus_tag="ACHKNB_RS15555"
                     /old_locus_tag="pgaptmp_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HGU4196576.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            9479..9943
                     /gene="fliL"
                     /locus_tag="ACHKNB_RS15560"
                     /old_locus_tag="pgaptmp_003112"
     CDS             9479..9943
                     /gene="fliL"
                     /locus_tag="ACHKNB_RS15560"
                     /old_locus_tag="pgaptmp_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HGU4196577.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9948..10952
                     /gene="fliM"
                     /locus_tag="ACHKNB_RS15565"
                     /old_locus_tag="pgaptmp_003113"
     CDS             9948..10952
                     /gene="fliM"
                     /locus_tag="ACHKNB_RS15565"
                     /old_locus_tag="pgaptmp_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HGU4196578.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVHQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10949..11362
                     /gene="fliN"
                     /locus_tag="ACHKNB_RS15570"
                     /old_locus_tag="pgaptmp_003114"
     CDS             10949..11362
                     /gene="fliN"
                     /locus_tag="ACHKNB_RS15570"
                     /old_locus_tag="pgaptmp_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HGU4196579.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11365..11730
                     /gene="fliO"
                     /locus_tag="ACHKNB_RS15575"
                     /old_locus_tag="pgaptmp_003115"
     CDS             11365..11730
                     /gene="fliO"
                     /locus_tag="ACHKNB_RS15575"
                     /old_locus_tag="pgaptmp_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HGU4196580.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11730..12467
                     /gene="fliP"
                     /locus_tag="ACHKNB_RS15580"
                     /old_locus_tag="pgaptmp_003116"
     CDS             11730..12467
                     /gene="fliP"
                     /locus_tag="ACHKNB_RS15580"
                     /old_locus_tag="pgaptmp_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HGU4196581.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12477..12746
                     /gene="fliQ"
                     /locus_tag="ACHKNB_RS15585"
                     /old_locus_tag="pgaptmp_003117"
     CDS             12477..12746
                     /gene="fliQ"
                     /locus_tag="ACHKNB_RS15585"
                     /old_locus_tag="pgaptmp_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HGU4196582.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12755..13540
                     /gene="fliR"
                     /locus_tag="ACHKNB_RS15590"
                     /old_locus_tag="pgaptmp_003118"
     CDS             12755..13540
                     /gene="fliR"
                     /locus_tag="ACHKNB_RS15590"
                     /old_locus_tag="pgaptmp_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HGU4196583.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13830..14453
                     /gene="rcsA"
                     /locus_tag="ACHKNB_RS15595"
                     /old_locus_tag="pgaptmp_003119"
     CDS             13830..14453
                     /gene="rcsA"
                     /locus_tag="ACHKNB_RS15595"
                     /old_locus_tag="pgaptmp_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HGU4196584.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14497..14685)
                     /gene="dsrB"
                     /locus_tag="ACHKNB_RS15600"
                     /old_locus_tag="pgaptmp_003120"
     CDS             complement(14497..14685)
                     /gene="dsrB"
                     /locus_tag="ACHKNB_RS15600"
                     /old_locus_tag="pgaptmp_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HGU4196585.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14848..15075
                     /gene="yodD"
                     /locus_tag="ACHKNB_RS15605"
                     /old_locus_tag="pgaptmp_003121"
     CDS             14848..15075
                     /gene="yodD"
                     /locus_tag="ACHKNB_RS15605"
                     /old_locus_tag="pgaptmp_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HGU4196586.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15373..16188
                     /gene="yedP"
                     /locus_tag="ACHKNB_RS15610"
                     /old_locus_tag="pgaptmp_003122"
     CDS             15373..16188
                     /gene="yedP"
                     /locus_tag="ACHKNB_RS15610"
                     /old_locus_tag="pgaptmp_003122"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HGU4196587.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(16185..17879)
                     /gene="dgcQ"
                     /locus_tag="ACHKNB_RS15615"
                     /old_locus_tag="pgaptmp_003123"
     CDS             complement(16185..17879)
                     /gene="dgcQ"
                     /locus_tag="ACHKNB_RS15615"
                     /old_locus_tag="pgaptmp_003123"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HGU4196588.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18117..18299)
                     /gene="yodC"
                     /locus_tag="ACHKNB_RS15620"
                     /old_locus_tag="pgaptmp_003124"
     CDS             complement(18117..18299)
                     /gene="yodC"
                     /locus_tag="ACHKNB_RS15620"
                     /old_locus_tag="pgaptmp_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HGU4196589.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(18378..19295)
                     /gene="yedI"
                     /locus_tag="ACHKNB_RS15625"
                     /old_locus_tag="pgaptmp_003125"
     CDS             complement(18378..19295)
                     /gene="yedI"
                     /locus_tag="ACHKNB_RS15625"
                     /old_locus_tag="pgaptmp_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HGU4196590.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKVKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            19468..>19642
                     /locus_tag="ACHKNB_RS15630"
                     /old_locus_tag="pgaptmp_003126"
     CDS             19468..>19642
                     /locus_tag="ACHKNB_RS15630"
                     /old_locus_tag="pgaptmp_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HGU4196591.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLR"
BASE COUNT         4943 a         5049 c         4926 g         4724 t
ORIGIN      
        1 agtggcaatt tctgctgtag ttgcatctta acgtataaac cagatttaat aacaacgaac
       61 tagatttaat aacaacgata tccgtaccta tccggatatc taatgtcata agcatccctt
      121 taattatccc ctcaacctct tgtaactttc ccactcttga actccacata gtccaaacgc
      181 cggatgcaaa aaatcacacg tgcatttctg aaattgtgat ggaggacgat agtatcaatt
      241 atatgtgctt ataaacgtcc ccgattagcg cagaagtcac cggattgttc aggctccaat
      301 aggttgagta tgccgtattt attttccaaa ataaaaatcg atgctgttgc tccttcataa
      361 aatactttta ctctctggac gcttttctta gctctcttgg tgttatctta aaacgattat
      421 ggaacctttc agtaaaacga gaaggacact tgtaaccatt ttccctggca atctcgctga
      481 tgggttttac tgtcgtttgt atagcagaca acgcattatt taacctcacg tcgtccaata
      541 tactttgaaa acttactccc tcgcttgcca gacggcgatg caatgtggaa acagaaatgt
      601 agagatatct cgctaccttg tttgctgtcc attttgttcc aggttcggat agcattaggt
      661 tataacaacg acttattaat gattgtttac tatctgataa aagtaaacaa ttaacatgat
      721 gtgctcctaa tgaaagtaga acgcccattg ctaagtgttc ctgaatttgc gttgagaagc
      781 ctcgggaaat agatgttttt agttgctccc aacaatatat taactcggga ttctgaggta
      841 agcagaaacc tgtcgtgtta cgaatttgat cggttatcgc ataaagattt tggaactttt
      901 caattagatc aatgggataa taaagcattt ctgcaagata aagacctgct tctgggtaat
      961 tcgcaatata aaattcatat ccacagggaa ataatattat ctgattatta tcaacagtta
     1021 gactatgcgt ctcccaattg ataacttttt ttccctgacg gatacgacat aaagctggca
     1081 taagaggctt aaccctgtga atctcatgat gtttatgcat ccgtatttct tcaatcttta
     1141 ggttagtcct atcttttgcc agcatactct tatcctactt tacctcataa acgggtatta
     1201 tctcagcggt tgcaatttta ttggcattaa gcatataacc aactaacgct ccgctggaat
     1261 tagaatccac aggaatctta tcagttttta gagcccatat tttaaactgg taatgatggg
     1321 gtttatctcc tttgggagga catgctccac caaatcccgc atagccaaaa tcatttcggc
     1381 cttggacagc accagtcgga agttttgtac catcacgtct tcctgcatca gcgggcaaat
     1441 atgttaccgt tgctggaata ttagcaacag tccagtgcca ccatccgctg cctgtaggcg
     1501 catctggatc atacacagtt acggcaaagc ttttggtgcc ttctggagca ccagaccagg
     1561 ttaatgaggg cgatgtatta cccccctcac acccaaatcc agaaaataca tgagacgttg
     1621 taagttgctc tcctgttttt atttcattac tagtgacctg aaatgctgcg gcctgcgcag
     1681 aaaatgttat gaatgccaat acacttgaaa cgataagttt tttcataaaa acctctttgt
     1741 tgtgacctat cgttatttta tttgatgtgc attgatctca ttatgcaaaa aggcgcaatg
     1801 atcgcgcaaa tgcaatcata attataaagt tgccccgaac attagccata atatgcacaa
     1861 atcagattcc tgttatctca aggatgatta ttttttaatg cggtgcgctt cgtgacaaca
     1921 agttaatgac taacacaccg gcacaaatca acatcatgcc tatgatggct ggcaagtcca
     1981 gtcgttggcc aaaaaatccc caggaaagca aactaatcag aacgataccg actcctgacc
     2041 agatagcata agcaatccct gtaggtatat aagccagtgt ctgagctaat aaccaaaatg
     2101 atgcacaata gcaaataatt gtgccaacag agggccataa ccgtgtaaac ccctctgaaa
     2161 acttcattaa ggttgtacca atgacctctg caagtattgc gccaccaaga taaatataag
     2221 ggttcatagc atattctttc ctgttcatac tggagagaat tgtactacag tttgaacaca
     2281 actcaccgtt ttcatcctgg tgtatgtgta tccagggggc tctggcgtgg caagagataa
     2341 aggcaggaac aataatgatc agaaggaacc ccaggctaac aaaagagttc actcttccta
     2401 tgagcaaagc gaagacaaaa catgttatcg cgtatacgga tcgtggatgg cagaaaactt
     2461 tcaggatcag gtatccattt taaaccagaa atagggtgag tttgccccat ccatgcccca
     2521 cgcgggcaga tgggatgtaa ttaacaacag gttacaaaac ctacacttgc atggacatca
     2581 cttcctgata cgccgccacc agcttattac gcacctgaat ccccatttgc atagaaactg
     2641 aggctttttg catatcggtc atcacatcgt ttaacgccac gccgggttca ccgagggtga
     2701 atttttctgc ctgcgtgcgg gcagctgttt gtgtatcgct aatgcgatcg agcgcggcgt
     2761 gcagctgccc ggcaaaacta atggtcggtt gcggcagtga atcctgcgca cgcgcactca
     2821 tcgccgtagc ctgtaactgg cttataaccc cttcaatccc ctgtatcgct gacattttcg
     2881 tctcctggat aatttctggt agcaaagcct accagtaagt caataagaca aaggcgctaa
     2941 atagcaacaa aaaaacgggt ttattggcgg atagaaaaaa acgaaagcac aaataatggc
     3001 agcgtcaatt tttcgagttt gctgacccgg gagtgagtct tgttccactt tgccaataac
     3061 gccgtccata atcagccacg aggtgcgcga tgaatgcgac tgcagcccag acaaaatctc
     3121 ttgagtggct taatcgcctg cgtgcgaatc cgaaaattcc attgattgtt gccggttccg
     3181 cggcagtggc ggtcatggtc gcactgatcc tgtgggcgaa agcccccgac taccgcacat
     3241 tattcagcaa tctttccgat caggatggtg gcgcaattgt cagccaactg acgcaaatga
     3301 atattcctta ccgcttcagc gaagccagcg gcgctattga agttccggca gataaagttc
     3361 acgaactgcg tctgcgcctg gcacaacaag gtttgcccaa aggcggcgcg gtcggtttcg
     3421 aactactcga tcaggaaaag tttggtatca gccagttcag cgaacaggtg aattatcagc
     3481 gggcgctgga aggcgagctt tctcgtacca tcgcaactat cggcccggta aaaggggcgc
     3541 gcgtacatct ggcaatgccg aaaccgtctt tattcgtccg tgaacaaaaa tccccttctg
     3601 catcggtgac ggtaaatctg ttacccggcc gcgcactcga tgaagggcaa attagcgcca
     3661 ttgtgcatct ggtttccagc gccgttgctg gtctgccgcc gggaaacgtc acgctggtgg
     3721 atcagggcgg acatctgtta acccagtcca ataccagcgg gcgcgatctt aatgatgctc
     3781 agttgaaata tgccagcgat gtcgaaggcc gtattcagcg gcgtattgaa gcaatcctgt
     3841 cgcctattgt tggtaacggt aatattcacg cccaggtcac ggcgcagctg gacttcgcca
     3901 gtaaagaaca aacggaagaa cagtatcgcc ctaacggtga tgaatctcat gcggcgcttc
     3961 gttcacgcca gcttaatgag agcgagcaaa gcggttccgg ttatccgggc ggcgtaccgg
     4021 gggcgttgtc gaatcaaccg gcacctgcga ataacgcgcc aatcagcacg cctccggcaa
     4081 atcaaaataa ccgccagcag caggcgagca ccaccagcaa tagcgggccg cgtagcacac
     4141 agcggaatga aaccagtaac tacgaagtcg atcgcaccat tcgtcatacc aaaatgaacg
     4201 tgggcgatgt gcaacgtctg tcagtcgcgg tagtggtgaa ttacaaaacc ttgccagacg
     4261 gtaaaccatt gcctctcagc aacgaacaga tgaagcaaat tgaagatctg acccgcgagg
     4321 cgatgggctt ttctgaaaaa cgcggtgact cgctcaatgt cgttaactca ccgttcaata
     4381 gcagtgacga aagcggcgga gaactgccgt tctggcaaca gcaagcgttt atcgatcagt
     4441 tgctggctgc cggtcgctgg ttgctggtac tgctggtggc gtggctgctg tggcggaaag
     4501 cggtacgccc gcagttaaca cgtcgcgccg aggcgatgaa agctgtacag caacaggcgc
     4561 aggcccgtga ggaagtggaa gatgcggtgg aagtccgcct gagcaaagac gaacaactcc
     4621 aacaacgacg cgctaaccaa cgtctggggg cagaagtcat gagccagcgt atccgtgaaa
     4681 tgtctgataa cgatccgcgc gtggtggcgc tggtcattcg ccagtggata aataacgatc
     4741 atgagtaacc tgacaggcac cgataaaagc gtcatcctgc tgatgaccat tggcgaagac
     4801 cgggcggcag aggtgttcaa gcacctctcc cagcgcgaag tgcaaaccct gagcgctgca
     4861 atggcgaacg tcacgcagat ctccaacaag cagctaaccg atgtgctggc ggagtttgag
     4921 caagaagctg aacagtttgc cgcactgaat atcaacgcca acgattatct gcgttcggta
     4981 ttggtcaaag ctctgggtga ggaacgtgcc gccagcctgc tggaagatat tctcgaaact
     5041 cgcgataccg ccagcggtat tgaaacgctc aactttatgg agccgcagag tgccgccgat
     5101 ctgattcgcg atgagcatcc gcaaattatc gccaccattc tggtgcatct gaagcgcgcc
     5161 caggccgccg atattctggc gttgttcgat gaacgtctgc gccacgacgt gatgttgcgt
     5221 atcgccacct ttggcggcgt gcagccagcc gcgctggcgg aactgaccga agtactgaat
     5281 ggcttgctcg acggtcagaa tctcaagcgc agcaaaatgg gcggcgtgag aacggcagcc
     5341 gaaattatca acctgatgaa aactcagcag gaagaagccg ttattaccgc cgtgcgcgaa
     5401 ttcgacggcg agctggcgca aaaaattatc gacgagatgt tcctgttcga aaatctggtg
     5461 gatgtctacg atcgcagcat tcagcgtctg ttgcaggaag tggattccga atcgctgttg
     5521 atcgcgctga aaggggccga gcagccactg cgcgagaaat tcctgcgcaa tatgtcgcag
     5581 cgtgccgccg atattctgcg cgacgatctc gccaaccgtg gtccggtgcg tctgtcgcag
     5641 gtggaaaacg aacagaaagc gattctgctg attgtgcgcc gccttgccga aactggcgag
     5701 atggtaattg gcagcggcga ggatacctat gtctgataat ctgccgtgga aaacctggac
     5761 gccggacgat ctcgcgccac cacaggcaga gtttgtgccc atggtcgagc cggaagaaac
     5821 catcattgaa gaggccgaac ccagccttga gcagcaactg gcgcaactgc aaatgcaggc
     5881 ccatgagcaa ggttatcagg cggggattgc cgaaggtcgc cagcaaggtc atgagcaggg
     5941 ctatcaggaa ggactggccc gggggctgga gcaaggtctg gcagaggcga agtctcaaca
     6001 agcgccaatt catgcccgga tgcagcaact ggtcagcgaa tttcaaacta cccttgatgc
     6061 gcttgatagt gtgattgctt cgcgcctgat gcagatggcg ctggaggcgg cacgtcaggt
     6121 catcggtcag acgccaacag tggataactc ggcactgatc aaacagatcc aacagttgtt
     6181 gcagcaagaa ccgttattca gcggtaaacc acagctgcgc gtgcacccgg atgatctgca
     6241 acgtgtggat gatatgctcg gcgcaacctt aagtttgcat ggctggcgct tgcggggcga
     6301 tcccacactc catcctggcg gctgtaaagt ctccgccgat gaaggcgatc tcgacgccag
     6361 tgtcgccact cgctggcaag aactctgccg tctggcagca ccaggagtgg tgtaatgacc
     6421 acgcgcctga ctcgctggct aaccacgctg gataactttg aagccaaaat ggcgcagttg
     6481 cctgcggtac gtcgctatgg gcgattaacc cgcgctaccg ggctggtgct ggaagccacc
     6541 ggattacaat tgccgctcgg cgcaacctgt gtcattgaac gccagaacgg cagcgaaacg
     6601 cacgaagtag aaagcgaagt cgttggcttt aacggtcaac ggctgttttt aatgccgctg
     6661 gaggaagtcg aaggtgtcct gcccggcgcg cgggtttatg ccaaaaacat ttcggctgaa
     6721 ggactgcaaa gcggcaagca gttgccgctc ggtccggcgt tattaggtcg cgttctggac
     6781 ggcagcggta agccgctcga tggcctgccc tcccccgata cgacggaaac cggtgcgctg
     6841 attaccccgc catttaaccc gttgcaacgt acaccgattg aacatgtgct ggacaccggc
     6901 gtgcgcccaa tcaatgccct gcttaccgtt gggcgtgggc agcgtatggg actgtttgcc
     6961 gggtccggcg ttggtaaaag tgtgctgctg gggatgatgg cacgttacac ccgcgccgat
     7021 gtcattgtcg tgggtttgat tggtgaacgc ggacgcgaag taaaagattt tattgagaat
     7081 atcctcggtg ccgaagggcg tgcacgctcc gtggtgattg ccgctccggc ggatgtttct
     7141 cctctcctgc gaatgcaggg tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat
     7201 cgtggtcagc atgtgttgct gattatggac tccctcaccc gctacgcgat ggcccagcgt
     7261 gaaattgcgc tggcgattgg cgaaccaccc gccactaaag gttatccccc gtcggtgttt
     7321 gccaaattac cggcactggt cgagcgtgcc ggaaatggca ttagcggcgg cggatcgatt
     7381 actgcatttt ataccgtgct cactgaaggc gatgaccagc aggaccccat tgccgactct
     7441 gcgcgggcaa tcctcgacgg tcacattgtg ctgtctcgcc gactggcgga agccgggcac
     7501 tatccggcta tcgatattga agcgtcgatc agccgcgcaa tgacggcgtt gatcagtgag
     7561 caacattacg cgcgagtgcg caccttcaaa cagctgttgt cgagttttca gcgtaaccgc
     7621 gatctggtta gcgtcggcgc gtatgccaaa ggcagcgatc cgatgctcga caaagccatc
     7681 gccctgtggc cgcagctgga gggctatttg caacaaggca tttttgaacg cgcggactgg
     7741 gaagcgtctc tccaggggct ggagcgtatt ttcccgacag tgtcataacc caggagataa
     7801 cggcagatgg cagaacatgg cgcgctggcg accctgaaag atctggcaga aaaagaggta
     7861 gaggatgccg cgcgcctgct gggtgaaatg cgtcgcgggt gtcagcaggc ggaagaacag
     7921 ctcaaaatgc tgatcgatta tcagaatgaa tatcgcaata acctcaacag cgatatgagt
     7981 gccgggatga ccagcaaccg ctggatcaac tatcagcagt ttatccagac gctggaaaaa
     8041 gccattactc agcatcgcca gcaacttaat cagtggacgc agaaagttga cattgccctg
     8101 aacagctggc gagaaaagaa acaacgtttg caggcctggc agacactgca ggaacggcaa
     8161 tccacggcgg cactgcttgc agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc
     8221 cagcgcgccg ccatgaggaa acctgaatga ttcgcttagc gcccttaatt accgccgacg
     8281 ttgacaccac cacattgcct ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt
     8341 tgttgagcga agcattagca ggcgagacaa ctaccgacaa agcggccccc cagttgctgg
     8401 tggcaacaga taagcccacg acaaaaggcg agctgctgat cagcgatatt gtttccgacg
     8461 cgcaacaagc ggatttactg atccctgtgg atgaaacact gcctgtcatc aacgtcgaac
     8521 aatccacatc aacaccgtta accaccgctc agacgatgac gttggctgcg gtggctgaca
     8581 aaaatacgac aaaagacgaa aaagcggatg atctgaatga agacgtcacc gcaagcctga
     8641 gcgccctttt tgcgatgttg ccgggttttg acaatacgcc caaagtgact gatgtgccgt
     8701 caaccgtgtt accggcagag aaaccaacgc tcttcacaaa actgacttct gcgcaactca
     8761 caacagcaca gcctgatgat gcccccggca cgccagctca gccattaaca ccgctggtag
     8821 cagaagccca gagtaaagcg gaagtcatca gcacaccgtc accggtgacc gctgccgcca
     8881 gcccgctaat tactccacac cagacacagc cactgcccac cgtcgccgcg cctgttttga
     8941 gtgcaccgct gggttctcac gaatggcaac aatcattaag ccagcatatt tcgctattca
     9001 cccgccaggg gcaacaaagt gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc
     9061 aaatctccct caaagtggat gataaccagg cacaaatcca gatggtttca ccgcatcagc
     9121 atgtacgcgc cgcgctggaa gcagcgcttc cggtactgcg tacgcagctg gccgaaagtg
     9181 gcattcagtt agggcaaagc aacatcagtg gcgaaagctt tagtggtcag cagcaggccg
     9241 cttcccaaca acagcaaagc caacgcacag taaaccatga acctctggcg ggggaagacg
     9301 acgatacgct tccggttccc gtctctatac aagggcgcgt aacaggcaac agcggcgttg
     9361 atattttcgc ctaacgtcag aggtagcacc gtaatccgcg tcttttcccc gctttgttgc
     9421 gctcaagacg caggataatt agccgataag cagtagcgac acaggaagac cgcaacacat
     9481 gactgattac gcgataagca agaaaagcaa gcgatcgctt tggatcccga ttctggtatt
     9541 cattaccctc gcggcctgtg ccagcgcagg ttacagctac tggcattcgc atcaggttgc
     9601 cgctgacgac aaagcgcagc aacgcgtcgt accctccccg gtcttctacg cgctggatac
     9661 cttcacggtc aatttgggcg atgcggatcg cgtgctttat atcggcgtaa ccctgcgcct
     9721 gaaagatgaa gctacccgct cgcggctgag tgagtatttg ccggaagtcc gtagccgttt
     9781 gctgttactg ttttcgcgtc aggatgctgc cgtactggcg acagaagaag gcaagaaaaa
     9841 cctgattgcc gagattaaaa ccacgctttc caccccgctt gttgccgggc aaccgaaaca
     9901 ggatgtcacc gacgtgctgt ataccgcttt tattctgcga taacgacatg ggcgatagta
     9961 ttctttctca agctgaaatt gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac
    10021 cgacagccag tgttagcggc gaaagtgaca ttcgtccgta cgatccgaat acccaacgac
    10081 gggttgtgcg cgaacgtttg caggcgctgg aaatcattaa tgagcgcttt gcccgccatt
    10141 ttcgtatggg gctgttcaac ctgctgcgtc gtagcccgga tataaccgtc ggggccatcc
    10201 gcattcagcc gtaccatgaa tttgcccgca acctgccggt gccgaccaac ctgaacctta
    10261 tccatctgaa accgctgcgc ggcactgggc tggtggtgtt ttcaccgagt ctggtgttta
    10321 tcgccgtgga taacctgttt ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg
    10381 agtttaccca taccgaacag cgcgtcatca accgcatgtt aaagctggcg cttgaaggct
    10441 atagcgacgc ctggaaggcg attaatccgc tggaagttga gtacgtgcgt tcggaaatgc
    10501 aggtgaaatt taccaatatc accacctcgc cgaacgacat tgtggttaac acgccgttcc
    10561 atgtggagat tggcaacctg accggcgaat ttaatatctg tctgccattc agcatgattg
    10621 agccgctacg ggaattgttg gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga
    10681 actggcgcga taacctggtg caccaggtgc agcattcaca gctggagttg gtcgccaact
    10741 ttgccgatat ctcgctacgc ctgtcgcaga ttttaaaact gaaaccaggc gacgtcctgc
    10801 cgatagaaaa acccgatcgc atcatcgctc atgttgacgg cgttccggta ctgaccagtc
    10861 agtacggcac cctcaacggt cagtatgcgt tacggataga acatttgatt aacccgattt
    10921 taaattctct gaacgaggaa cagcccaaat gagtgacatg aataatccgg ccgatgacaa
    10981 caacggcgca atggacgatc tgtgggctga agcgttgagc gaacaaaaat caaccagcag
    11041 caaaagcgct gccgacgcgg tgttccagca atttggcggt ggtgatgtca gcggaacgtt
    11101 gcaggatatc gacctgataa tggatattcc ggtcaagctg accgtcgagc tgggccgtac
    11161 gcggatgacc atcaaagagc tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg
    11221 tctggcgggc gaaccactgg atattctgat caacggttat ttaatcgccc agggcgaagt
    11281 ggtggtcgtt gccgataaat atggcgtgcg gatcaccgat atcattactc cgtctgagcg
    11341 aatgcgtcgc ctgagccgtt agtgatgaat aaccacgcta ctgtgcaatc ttccgcgccg
    11401 gtttctgctg cgccactgct gcaggtcagc ggcgcactca tcgccattat tgccctgatc
    11461 ctcgctgctg cctggctggt aaaacggttg ggatttgccc cgaaacgcac tggcgttaac
    11521 ggtctgaaaa ttagcgccag tgcttcgctg ggcgcgcgtg aacgggttgt ggtggtcgat
    11581 gtggaagatg cacggctggt gctcggcgtt accgcagggc aaatcaatct gctgcataaa
    11641 cttccccctt cagcaccaac agaagagata ccgcagaccg attttcagtc ggtcatgaaa
    11701 aatttgctta agcgtagcgg gagatcctga tgcgtcgttt gttgtctgtc gcacctgtcc
    11761 ttctctggct ggttacgccc ctcgccttcg cgcaactgcc gggtatcacc agccagccgc
    11821 tgcctggcgg tggacaaagc tggtcgctcc cggtgcagac gctggtgttc atcacctcgt
    11881 tgacgtttat tccggcaatt ttactgatga tgaccagttt cacccgcatc atcattgttt
    11941 ttggtttatt gcgtaacgcg ctgggaacgc cctccgcgcc acctaaccag gtattgctgg
    12001 ggctggcact atttttgacc ttttttatta tgtcaccggt gatcgacaaa atttatgtcg
    12061 atgcgtacca gccattcagc gaagagaaaa tatcaatgca ggaggcgctg gaaaaagggg
    12121 cgcagccgct acgtgagttt atgctgcgtc agacccgtga ggcagattta gggttgtttg
    12181 ccagactagc gaataccggc ccgttgcagg gacctgaagc cgtgccgatg cgcattttgc
    12241 tcccggccta cgtgaccagc gagttgaaaa ccgcatttca gataggcttc acgattttca
    12301 tccctttttt gattatcgac ctggtgattg ccagcgtgtt gatggcattg gggatgatga
    12361 tggtcccccc cgccaccatt gctctgccct ttaaactgat gctgtttgta ctggtggatg
    12421 gctggcaatt gctggtcggt tcgctggcgc agagctttta cagctagaga ggcaaaatga
    12481 cacctgaatc ggtcatgatg atggggactg aagcgatgaa agtcgcgctg gcactggctg
    12541 ccccgctgtt gctggtggcg ctggtcacgg gccttatcat cagtattttg caggccgcca
    12601 cccagatcaa cgaaatgacg ctgtcgttta ttccaaaaat catcgccgta tttattgcca
    12661 ttattattgc cggaccgtgg atgctcaatc tgttgctgga ttacgtccgc accttgttca
    12721 ctaacctgcc gtatatcatc gggtagcccg tacaatgatg caggtgacaa gcgatcaatg
    12781 gctttcctgg ctaagtctgt acttctggcc gttactgcgc gtgctggcgc tcatttctac
    12841 cgcgccaatt ctgagcgaac gcagcgtgcc gaaacgggta aaactgggtc tggcaatgat
    12901 gatcacgttc gctattgccc catcattacc tgccaacgat gttcctgttt tttcgttctt
    12961 tgctctgtgg ctggccgtgc agcagatcct gatcggcatt gcgcttggtt ttaccatgca
    13021 atttgccttt gccgctgtgc gaaccgctgg cgaaattatc ggtctgcaaa tggggctgtc
    13081 atttgcgacg tttgtcgatc cggccagcca tctcaatatg cctgttttag cgcgtatcat
    13141 ggatatgctg gcgttactac tgttcctgac atttaacggt catttatggt tgatttcact
    13201 gctggtcgat acctttcaca ccctgccaat tggtggcgaa ccgttgaaca gcaatgcgtt
    13261 tctggcactc accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc
    13321 gctcattact ctgctgctga cactgaatct ggcattaggt ttacttaatc gtatggcccc
    13381 gcaattatcc atttttgtta ttggatttcc attaactctg actgtcggta tctctttaat
    13441 ggcggcatta atgccgttaa ttgcaccttt ttgcgaacat ttattcagtg agatgtttaa
    13501 tttgctggct gatattatta gtgaattgcc attaatataa ttccgtaacg tttatcatgt
    13561 tatcctaagg attatccgaa aaataatacc tgcgaacatc ttccaggata ctcctgtggc
    13621 gaaatatttg ttttaagctc actcacatat cgcaacattt actttacttt aagacaattc
    13681 caggcaaatt atacaacact ttacgggata gtaagtccgc ctgaaaaatc gcgagagtgg
    13741 cgcattaggt gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat
    13801 tatcgttacg cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt
    13861 acacccgact aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca
    13921 acgacattga aaccgttgat gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg
    13981 tgtttattaa tgaggactgt ttcatccatg atgcttctaa cagtcagcac atcaagcaca
    14041 tcattaatca acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt
    14101 ttgatgaata tctattggtc agaaaaaatt tattgataag ttctaaatcg attaaaccgg
    14161 aatctctcga cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcttttt
    14221 taaatatgcc gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg
    14281 caggtcaggg aacgattcaa atctctgacc aaatgaatat caaagccaag accgtttcat
    14341 cgcataaagg taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg
    14401 tcgtccgact gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc
    14461 tgactggtgg tttcccacca gtcaggctga ataagattac tctgctttct ccacaaagat
    14521 accgtcctga tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga
    14581 aaccaggtac attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg
    14641 cggaccgcca tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg
    14701 gctttgtgcc agtgtagaac aatttcgttt ttctggcagc gccaggcgcg cgcgagtgct
    14761 gattttctcg acggtctata cttaagagat gccaggcgga cttaacgact ggcggcaaca
    14821 acagagtaac ggttgcgagg aaagatgatg aaaaccgcaa aagagtacag cgataccgca
    14881 aaacgtgagg tcagcgtcga tgtcgatgcc ctgctggcgg cgatcaatga aattagcgaa
    14941 agcgaagttc atcgcagcca gaacgattct gaacacgtta gcgtcgatgg acgtgaatat
    15001 catacatggc gtgaattggc ggatgccttc gaactggata ttcatgactt cagcgtctct
    15061 gaagtgaatc gttgaatgca caataaaaaa atcccgaccc tgagggggtc gggatgaaac
    15121 ttgcttaagc aagaagcact taaaaaattc gttacaccag gaaatctgat gtgttcatca
    15181 ccttatccgc aatttttttc gctgacaaga aaatattcgc gatatgaatg attaacgcta
    15241 cttatgaata gaaatgtgac gtgcgtcacc ccttggtggc tctggcgtca acgggatcgc
    15301 tcccgaaaaa atcatctttg attatttgtg ctgttttgag aatcatcctg gtaaggggag
    15361 aactgcgcga ccatgttttc aattcaacaa ccactactgg tttttagcga tcttgatggc
    15421 accctgctgg acagtcatag ttatgactgg caaccggctg ccccctggct cagccgttta
    15481 cgcgaagcga atgttcccgt cattctctgt agcagtaaaa cttcagcgga aatgctgtac
    15541 ttgcaaaaaa cgttggggct acaagggtta ccactgattg ccgaaaatgg cgcagtgatc
    15601 cagcttgctg aacaatggca ggatatagac ggttttccac gcatcatctc aggtattagc
    15661 catggcgaaa tcagccaggt tttaaatacg ctacgcgaga aagaacattt taaattcacg
    15721 actttcgatg atgtcgacga tgcaaccatc gccgaatgga cgggattaag ccgtagccag
    15781 gcggcgctga cgcagctgca tgaggcgtcg gtaacgctaa tctggcgcga cagtgacgag
    15841 cgtatggcac aatttaccgc tcgtctgaac gaactgggct tacagtttat gcagggtgcg
    15901 cgcttctggc acgtactgga tgcctctgcc ggaaaagatc aggctgccaa ctggattatc
    15961 gcgacctatc aacaattgtc aggcaaacgc ccaaccacac ttggcctggg cgatgggcca
    16021 aacgatgcgc ccttactgga ggtaatggat tacgcggtga ttgtgaaagg gctaaaccgt
    16081 gaaggggtgc atctgcatga tgaggatccg gcccgcgtct ggcgaacgca gcgtgaagga
    16141 ccggaaggct ggggtgaagg gctggaccat tttttctccg cccgttaagc gttatcgctc
    16201 gcgcataccc gattacgccc ggcctgttta gcgagataaa gtcgacggtc agccagtgac
    16261 tggagttgtt caaaatcata atcaccggtt tcctcgctgc tacttacccc cagcgaggca
    16321 ctgatgcgta tcgtcgtact cttagcaatc aacatctctt tttcatttaa cttcaggcga
    16381 atacgttctg cgacttccgc agcctccgtc agactcgcgc ctggcagaat cacacaaaac
    16441 tcctcaccac cgacccgccc ggcaacgtcc tgcgcacgca aggaactgct aattaatccg
    16501 gcagcatgag aaagaacacg gtcgcccgcc tgatgaccaa agcggtcatt aatcgcttta
    16561 aaatggtcaa gatcgacctg aatgacagaa aaaggatgtt ggtgcgtctg atacaattta
    16621 gcgagcggac gggctttttc gaacagtgcg ccacggttat ataaacgcgt taaggtgtcg
    16681 tgccacgcct gccactgcaa cgagctttgc agaacataca tgttgctaac catccggcga
    16741 atcacatacc aggagatgag taacatggtg gtaaagagcg cccacagcag ggttaatgca
    16801 atgctgatac tgccgaaatc gccgcgcacg ccttcgctta acgtatggac gcgcaccagc
    16861 acaccgtcga aatggtccag acgttcccag ctaacatagc gactgtccat acgaatgccg
    16921 ccccgcgtgt catgttccat cgcctgcgcc agcaaggcca gttcacgagg atcaaaaata
    16981 ttccctgttg gatggtcagg attggaagag gtcaaaaatc tcagcttact gtcatagagc
    17041 tgatactcac catcgaggtt tttatcgatg gcgtttctta aaaattgctg catggtacgc
    17101 acgggaatac tcatccccag cacgccatac cagtagttat tactgtctac tggaacactg
    17161 acggtaacct gcggttcagt attgctggcg tgttccggtt gcgaagtaaa ccagcgtacc
    17221 gcgcggtgac gattttctcg ttgcgaatgg ccgataaacc agggttgggt gacatagccg
    17281 taataacgcg ttggtacatt gcgcgtaaac aaggtcggct gcgtcgaaac gtaaaatccg
    17341 gcacgcgaga catacatcgc ctgttcaacc atcgacgagg tgttgtgcgc cagtcgcagc
    17401 aagtaaccaa cttccagtgc agcggtaatt tcattgtcga ggctttcatt ttcgcgagac
    17461 aggagattcc cctcgctgac taatgcatcc gatacgccat tgactgacag ggtgcgtcgt
    17521 cggttgagtt cgattttcca ggcgtgctcg tcgcgatgct gttcgaactc ggttacagca
    17581 tcacgcagag aggtgaaatc cagtggcgct acgagagctt cgcgcatgcc attacgcaaa
    17641 aagatcagtt tgtcgacgtt atactgcaaa tgcttatcga gcgcgttggc tacgttttcc
    17701 agatgattac gctggctgga gatataggca tcttccagca ccactacttc acgccaggtg
    17761 agcaaggtgg aaaaaagcaa taccacgata aagcagagat taacgacatg accaggcccc
    17821 aggcggcgtg cgagtttttt caaccagctc tggttttcca tttttgtctc gtgctgcacc
    17881 ctgacgactc ccaacctgct tttttatgat tctggcataa cctggcgata gcgtaccgca
    17941 aaacagcgat aattttataa tggtggcatg ataagacgcg acagacatcg atagttttat
    18001 aacgtaggca tgataagacg cgatagcgtc gcatcaggca attcagtgca gacactggca
    18061 cacccgaccg gatgcgacgt gagcgccaca tccggtcggg tgttaatgat accctttcaa
    18121 acttcttccg caggacgact tccctcccct ggaacaagct cggtttcatg aaaagcttcc
    18181 cgcttgaccc cgtaaccgtc ataccaacga cactcaacca taccgctgga gtatccggtg
    18241 acaatcatcc gcgggccgcc ctctttaacc gtaacttcct cactaaccat aaagctcata
    18301 ctcgcctcct tttttcgagt gaaacttgtt caccttagtt gaagatggcg aattttgcat
    18361 aatagccagt gcgagtatta atgtgcctga ccgcgtattt tcgctacggc tttcactccc
    18421 agcaccacga tgccgccgat gataaatcca agaatcagat ttaaaacagt cggtaatatc
    18481 atcgccacca ctgcactttg ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca
    18541 ataccatgta ccacaatccc gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat
    18601 aacgccttca tcaaccaggg cgcaatgatc aataaccctt tacctaatgc ctgcatcagc
    18661 gcgctggatt tttccgccag ccaatacccc aggtcatcaa tcttaacgat aacccctacc
    18721 agaccgtaga cgcccacggt cactaccagc gcgatgcctg aaagcaccag cacctgatta
    18781 agcaacggcg cttcggccac aatccccagc gtgatggcga cgatttcggc agacaatata
    18841 aaatcggtac gaatcgcccc ttttactttg tccttttcaa acttcagcgg atcctgcgcc
    18901 gccagcttct ccagacgctg ctggctctgc gccggatctt ctttatgttt acgcgcctcc
    18961 agcatatgca gcactttctc tactccttca aagcagagaa acgcgccacc aatcatcaac
    19021 agaggcgtaa tcgcccacgg gataaacgca ctgatgatca gcgccagcgg caccagaatc
    19081 actttattaa tcagcgatcc tttcgccacg ccccagacca cgggaagttc ccggttggcc
    19141 cgcacgcctg aaacttgttg cgcattgagc gataagtcat cccctaatac accggcggtt
    19201 ttcttcgccg ccagtttgcc catcacggag atatcgtcca gtagtgtggc gatatcatcg
    19261 agcaacgtca gtaaactact cccggccaaa agcattccct ctcattttta ttgttgaagg
    19321 gtgtaagtat gaagcaaaaa cggcagctct gatacttgct tcacaggtca acattttttt
    19381 aacaattaag tgcaattata tctctcgcca gcagaatgat tttcacgttt actatgaacc
    19441 acctttttat ttccaccgtg agggattatg cgtttccgcc agttgttacc gctttttggc
    19501 gcgctgtttg cgttgtatat catttggggc tcaacctatt ttgtcattcg gattggcgtg
    19561 gaaagctggc ctccgttaat gatggcaggc gttcgattcc tggcagccgg tattttattg
    19621 atggcatttt tgctactgcg cg
//
LOCUS       DAZFVO020000095        18919 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFVO020000095 DAZFVO020000000
VERSION     DAZFVO020000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.096"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(29..2029)
                     /gene="yehQ"
                     /locus_tag="ACHKNB_RS15635"
                     /old_locus_tag="pgaptmp_003127"
     CDS             complement(29..2029)
                     /gene="yehQ"
                     /locus_tag="ACHKNB_RS15635"
                     /old_locus_tag="pgaptmp_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HGU4196592.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2026..3162)
                     /gene="yehP"
                     /locus_tag="ACHKNB_RS15640"
                     /old_locus_tag="pgaptmp_003128"
     CDS             complement(2026..3162)
                     /gene="yehP"
                     /locus_tag="ACHKNB_RS15640"
                     /old_locus_tag="pgaptmp_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HGU4196593.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3155..5434)
                     /gene="yehM"
                     /locus_tag="ACHKNB_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /pseudo
     CDS             complement(3155..5434)
                     /gene="yehM"
                     /locus_tag="ACHKNB_RS15645"
                     /old_locus_tag="pgaptmp_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(5445..6533)
                     /gene="yehL"
                     /locus_tag="ACHKNB_RS15650"
                     /old_locus_tag="pgaptmp_003130"
     CDS             complement(5445..6533)
                     /gene="yehL"
                     /locus_tag="ACHKNB_RS15650"
                     /old_locus_tag="pgaptmp_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HGU4196594.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(7108..7428)
                     /gene="yehK"
                     /locus_tag="ACHKNB_RS15655"
                     /old_locus_tag="pgaptmp_003131"
     CDS             complement(7108..7428)
                     /gene="yehK"
                     /locus_tag="ACHKNB_RS15655"
                     /old_locus_tag="pgaptmp_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HGU4196595.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            complement(7489..11121)
                     /gene="yehI"
                     /locus_tag="ACHKNB_RS15660"
                     /old_locus_tag="pgaptmp_003132"
     CDS             complement(7489..11121)
                     /gene="yehI"
                     /locus_tag="ACHKNB_RS15660"
                     /old_locus_tag="pgaptmp_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HGU4196596.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            complement(11131..14924)
                     /locus_tag="ACHKNB_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /pseudo
     CDS             complement(11131..14924)
                     /locus_tag="ACHKNB_RS15665"
                     /old_locus_tag="pgaptmp_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(15065..17098)
                     /gene="metG"
                     /locus_tag="ACHKNB_RS15670"
                     /old_locus_tag="pgaptmp_003134"
     CDS             complement(15065..17098)
                     /gene="metG"
                     /locus_tag="ACHKNB_RS15670"
                     /old_locus_tag="pgaptmp_003134"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGU4196597.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            17230..18339
                     /gene="apbC"
                     /locus_tag="ACHKNB_RS15675"
                     /old_locus_tag="pgaptmp_003135"
     CDS             17230..18339
                     /gene="apbC"
                     /locus_tag="ACHKNB_RS15675"
                     /old_locus_tag="pgaptmp_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HGU4196598.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            18602..18883
                     /gene="yehE"
                     /locus_tag="ACHKNB_RS15680"
                     /old_locus_tag="pgaptmp_003136"
     CDS             18602..18883
                     /gene="yehE"
                     /locus_tag="ACHKNB_RS15680"
                     /old_locus_tag="pgaptmp_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HGU4196599.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         4454 a         4851 c         4924 g         4690 t
ORIGIN      
        1 agtagaactc ttagtaaaaa gcactatttc atccttgttg ctgaagcatg gggaataatt
       61 gttcgcaaag taaaacaccg ttattcattg cttctacccg tgcctcgctt tctgtattac
      121 gaaattgtcc caacacatgt gccagccgat aaaaaccgac cgcggagagg tcattcgcca
      181 gcaactctgc ctgactaata gcgctctgtt cctgatagcg ccagccgtta tggagcagtt
      241 gaataagtaa cgcctggcag cgcatcagca actgatgagc ggtagacggc acaggcaaaa
      301 cgctggcaga aggtagcggt gccacaggcg cagtttccgc gtccagcgcc caggcgcggg
      361 tttttgtcat catcacccgt ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc
      421 cagaaaccag actcacgggg tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg
      481 caaaaggaga acaggcagat gccggaaggg ataacgtcag gagattatct tcaccttcgc
      541 cgctgattac ctgcgcatcc agcgtctggc ggctgctatc ccaaccgagc gaaatacact
      601 cagcgaccgg cagaataaat aagttatcga cctgattaag aggccgtatg caggcggggg
      661 gacgctggcg taaatattcc cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca
      721 acatttgcca ggcatcaggc gacagcggca caacgctgct taagcggttg cgggtagcta
      781 acagcagctc gccatcggca ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca
      841 gtgcgccagc ctgaaaacta aacagccgac gcgtagctgc cggtgagttt tcctgttcac
      901 ttcgcggcca actgcgcgaa aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga
      961 tgcgtaaacc ataatgctca atatcctgcc agcaacgcat acctaaagac accagccgca
     1021 gatgatcaag ctttgcttct ccggcaatgc cagagccaac gaccgtgcgc cacggcacag
     1081 gaggaacttc accaacactg tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt
     1141 ttaatgccgc aagctgacgt aagcattctc cggcatgata gtggctggcg cgagtgtgga
     1201 aggcatcaac gcttgcccgt agctgtcgta gtgattcact cacccagcgc cagttgcagg
     1261 cctctgccgc ctgcaatgcg cggttgaatg ctgcctcgta gtgaataagc ggctggctga
     1321 tgccgccaag ccataatgcc tggcttaatt gctgaacata ttgacgacac gtttggcctt
     1381 cttcgctggc aaacggatcg tcagatgatg tgacgtgttc gctgcgcatc tgccagatta
     1441 aatggttaaa ttctgcttgc tgcgctttgg cctcgacgaa ggcctgcaca gccagtacga
     1501 catgttcgca aagtgtgcct tcaatacaat cacaacgggc gaaacgaata ctgctacggg
     1561 aataaaaacg cacatcgctc atcggtaagc gggcagaggg aatttcgccc ggcgcacaga
     1621 acaactcaat ggtgatgcct ttagcgacca gcgcctgcgc gcgtttgcgg gtggcatcgg
     1681 gcagggtagc cagttcttcc agccagattg ccggatccca ctcttcttct ttttccgtgg
     1741 gctggacggt ggcacaaagt cgttgataac ttaacaccag catcacgcga tgacggcaca
     1801 tgccgctggc cccgcagctg cactgagcct ctttcagtgc ctggctgttc gccagctggg
     1861 tacggacacc gtcactgaag gtggcgatta aagcgccgtt ctcatggctg atttccggga
     1921 cgttgccatt ttccagttcc ttaagactgc gcttaacaaa accggcattg cttaacgccg
     1981 tcagtgcctg cggtgtcagt tctaataatt ccggacgtag tgaattcatg actgaagatt
     2041 ctccgcaagc catgatgcca gctcgcccgg cgtcatggcg gctatttgtg cgccgacatt
     2101 aaccagcgcc tgggccgtat cgcggtcata gcaaggcgtt gcggtgctat cgagcgctgc
     2161 cagtcccagc actttgatgc cgctctggac acactttttc acctgatgcg tcagcaatga
     2221 tgatgaaccc ccttcgtaaa aatcgctcac gaggataatg acgctttttg ctggttgttc
     2281 aataagttgc cgaccatact ccacggcact ggcgatattg gtcccgccgc ccaactgtac
     2341 tttcattaat aactctaccg gatcggcaac gtctgccgtg agatcaacca cgcttgtgtc
     2401 aaacgccacc agatgggtac gaatgccggg taactgccac aaacaggccg ccatcaccgc
     2461 agagtggatc accgagtcca ccatcgatcc gctttgatca accagtaaga ccagttgcca
     2521 ttgttcgctt tggcgtttaa tgcggctgtt aaagcggggg gattcgatat acaacttgcc
     2581 gtgttgcggg tgccagtgtt gcaggttggc gcgcagagta cttttgaaat caaagtttcg
     2641 cgccagtgga attaatgagc ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac
     2701 ttcctttgcc agtcgcgcca taatttcttc aacaacctgg cgcactatct ggcgggcggc
     2761 agccagtact tcgggattca tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc
     2821 cgaaggctgc atacgttcca gcacgtcgag atttgtcacc acatcttcaa tgccgtagcg
     2881 cagtacggca tcgctttcca gccgctcaat cacctgttgc ggaaacagcg tgtggatact
     2941 gttgatccac tcaggagtgg tgagatttga gccacctaat ccaccagagc gttcaccacg
     3001 ctggagccgt tcaggatcgc gcccatacag ccactccagc gcgtgatcta tctgccgggc
     3061 gttgtcatcc agcccacaaa gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg
     3121 ttgtagctca cgggtggtca gaagatcgtt cagttcagac atggaaaact ccccagtgtt
     3181 gcagcgatgc cagtgcctgc tgttcgagtt gttgatgatg tgcaatggct tgcggtggac
     3241 aatgcaacgg catttgcagt gccgaaacgg gaagttgcgc cagttgataa tgctcaagca
     3301 cctgatgcgc cagcgttccg cgttctcgtg gcggtagcca ggccatcgcc gcgcgtaaat
     3361 cgggcagggc attgataaaa ttggcatcac tcagttgatt caaatggctg ctgaaaccgg
     3421 cgataaatgc aggttgacag gccagttgat gacgggctaa tgccagcaaa ccgtgcagcg
     3481 cctcgccgga ttgtgccggg gataactgcg ccagcatcgt cagcgccgct ttggcactgg
     3541 cgttgggatg ttccagacgc attagtgcgc caagagccgc gccgcgatcc agcgccgggg
     3601 catgaatcgc ctgactgcgt cgctccagta aggctaccgc cgcagaaagc gaaacgccgg
     3661 ataaattaac gccgctatgt agatcgcgca gaatatggca aagcgcttgc cagctatgca
     3721 ggtgagcgtg aaactccttt tcatccggtc tgccgttaga ttcacacagc cacagcgtgc
     3781 gatcgatagc cgcgcataac gtcgtttgta atatctgcgc gccttgcata ccgctaattt
     3841 catccagccg ccataaggca tatagcactt ccagcgcctg gcccatttcg gcaaattgat
     3901 tttcctgggc gattaataat gtgagttgct ccagtaattg ttgactgaag gacgccagac
     3961 ccgctaacgc cgcctggcta agacatgtgg tgatactgcc gattccaccc gcgtccagca
     4021 tatcggcttc taatttatgg cgtgcggctt cctgaagtgt tgcaccaaag catgcggcct
     4081 caatcaatgc cgcatgctgg ctaagcgggc gggttaattt ccagtgttct tcaccgttgc
     4141 ctgcaagtgt cagtgtactt cactgctggc gtacaatccc agggatctcc agtattgcca
     4201 gccgatgtaa cacctgactt tgcgccagcc catcgggggt aaagcgattt aaggtaagct
     4261 ccgccggaag agagattgcg gtacgttcca gttcgcaggt gacatctttt ggcagaggcg
     4321 gttgtggcgt ggaaggggca agtttaccga atccgtcacc cgctaatgtg tctatcaacg
     4381 ttagcagaat cggatcggta tcgggatgaa taacgccgcg atagctccac ggcaacggtg
     4441 cattcagggc ttctttaatc aacgagcctg ctatggcatc cagccagtca gtgcgtaacg
     4501 gtagtgtatg accgcgcaac tgtgccagtg ccattgcatg cagatgtgcg gcagccatat
     4561 ccgcggtcga agcaggcaag ttgtgctggc gcaaacgggt gagaaccgtt tttagcagtt
     4621 gttcaccggc ctgctgtaag ccccactgcc agcaccagtt ttgccagacc ggggcaggca
     4681 taccggaaag gtatcctgcc agcacatcaa ggcgcttttc actgtagggc gtgagataac
     4741 aacctgtaat ggcatctgcc agcgagggca attccggcgt gttaatgtcc tgcgggcatt
     4801 cgcgccacat ttttgccagt gccggagcgt gccagccacc gcagacgact aacacgtcgc
     4861 cattattttg ctgcaccgcc catgcaatcc agcgagccat aaaggcttcg cgctgacgat
     4921 tgagtgcatc gccggggaaa tcgccccgca gttgggcaaa atagtgcgcc agcgcagagg
     4981 gtaatgcagt ttgctggctt tcatcttcga acaagtgatc ccacagggta tcgctgttat
     5041 ccatgcgggt ggcacgcagc agtaaggcct ggctttcatc ttgcgtatca ggcgaatcgt
     5101 cctcttcttc actctgcgcc cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac
     5161 gacgtgcggc ttgtagcgcc tgccactccg gcgaaaattc agcaaatggc gtccaggcac
     5221 cacgcccggg ggctgcaccg tcctgatact ggcaataact gtaaatagct accggaagct
     5281 ggtgggaaaa aaaaagttcg tctacccggt cattaaaatc agccgggcct tcaatcaaca
     5341 cgtatcgtgg ccgctggctt tcgattaacg atttcaccag ccgggcgcag gccggactat
     5401 gatgccggat gccgacaatt aacggctcgc tcatgcctct ttcctcacgg caggcggtgg
     5461 cgggcttgat aataagcctg ccagtgagct tctttatgtg tggcgacacg ctgttcgaag
     5521 taacggcgca gacgagcgcg atcttcttcg ttatctttga cgatggttcc ggcaatacag
     5581 tccaccaggt cggcaggctc ccccgcgcga ttcgctaaaa accaggcgcg gacgcctacg
     5641 gcgtgggcca cgttcacggc ttcagcggtg gacataatcg ctgtcagtgt atccattgac
     5701 gttttctttt cgccgttggc acgcagatcg cggaaggtgc gtaccagtaa ctccagtact
     5761 gcgtcaggca ctttatgcgg aataccgcta tgggccagca aacgcgccga agcactggcg
     5821 accagttcca gctcctgggc gaaatccata atcggaaaga cggtttcaaa atcgaagcga
     5881 cgctttagcg cggcactcat ctcattgacg ccgcgatcgc gggtattggc agtggcaata
     5941 atattaaagc cttcccgcgc atagagctga ctggcttcac cggtgagttc cggcaccgtc
     6001 atcaccctgt cagaaagcat acctaacaga cagtcttgta cttccagcgg tgtacgcgtt
     6061 atctcttcaa aacggacgat tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc
     6121 agtgcttccg ttgacggacc atggttgata agcagggcgt agttccagcc gtacttgatt
     6181 tgatcttcgg tagtagatgc gccgccctgg atggttaacc cggcatcgcc gctaatagcg
     6241 gttgccagta attcagacag gagagatttt gcggtgccag gttcccccac caacatcaaa
     6301 ccgcgcccgg tggcaagagt gactaacatg cgttcgacag cggagggact aatcaccact
     6361 ttacggctaa tattttgcgc gctatcgcca aggataaaag cacgggccgc aggcaaactt
     6421 aactgccaac cgggcgggca aggtgcgtta tcattttgtt ttaattttgc cagttcatcg
     6481 gcgtataaca cagcagcagg cggacgctgc agatgattat tctgcggtga catatattca
     6541 tccctgaaat gtattaatga aataaacgat attttacgcg caataaatgt ttggcgcatt
     6601 agttgtttga gcgaaaaata tctggaaagc agatcacagt aaataaaaaa acctgctggt
     6661 gaggcaggtt cttttattta ctactttcag gcctaacacc acagcctgtg caacatcgtg
     6721 ttccaacgaa cgaattataa ccacggctgg accccgttgc aattaagata atgagattta
     6781 taatccagac cgttgggacg ttgcgccgga tcgaatctat ttcaagtcct ggctaccgga
     6841 cgggtggtgc tgcaggtggt gccctgatcc ggagtgcaac atcgtggtcc aacatacgta
     6901 aaccagtatc gacaggcagt gtaagggtaa ggtgctggtg ttgcgctcct taataaggag
     6961 agcggatgga cacgctgaca caaaagttaa ccgtgctcat tgctgtactg gagctattag
     7021 tggctctgtt acggttgatt gatttgttga agtaacgggc aaattgaaac gcatctaagg
     7081 gggaaaaccg cttccccctt taataaacta atattctatt agttggttga atatattctg
     7141 tagttcacga ttaaaaattg ctattacagt ttcaggtgat tcacctcgta atttccgctc
     7201 atcaatacac tcatctctta tataaatcaa cgcatcattt attagcgggt cagcatcttc
     7261 tcgatcacgt tgatgaaatg ttggacgtag ccggaatcgc tccagtcgat agacggaacc
     7321 tgtaaataat tcagagcatt tatggatctc aagtctgaag gaaaacggat cttcaggtac
     7381 agggacctca taatcgtaaa tagcaaccag ctctttttgc acgatcatcg ctttccccgc
     7441 tggtaagtcg tgttgataac gggcagttgc atcactgccc gctgtgtttt aatggaaaat
     7501 gacgtcaggt gcgcttaacg cctcactcaa atcaaacaca tcaagttcgg caaaagtgac
     7561 ggatttattg tcactgtaca ctcgaagaga acggaagctc attagcgctt tgctatcaac
     7621 attgtagtgg ttaaaaccgg gtgaaaactt aaggaccagc cggtactcac ccaggtcata
     7681 gacaaatgcg tcctcatagc ctgactccca gcctttgtat cgcattccca taagttgccc
     7741 aacggtagca gatttacctt cccagcgatt caaactatta ctggctgact cgtcaggcgt
     7801 gagcagcacc gtgcgccgtg tcaactggcg aagtggcggt ataatttcgt aatcggcaaa
     7861 aagctgggca aattcactgc gcatttctgc tgtcatttct aacgggtggg caatgccaat
     7921 cagagcatcc gcaggcaggt caattggctc atcctgcgca ttgcagaact ccccctctgc
     7981 ggccacacga aaggcgttga gtaaacgacg cggttcattt gccggataca cgccccatat
     8041 taatcgctgg gtaaccaggc gggtaaaggg atgattaaca aacagagtat gaaaatctgc
     8101 cagcgaccag cgtcgggtgg tacgtagggc agcttcaaga cgcgggagca ggtttttgct
     8161 cacctgagta gcatcttttt ttagcccttt tagtcgggcc agtgcctcgg gcgctttcag
     8221 ttgatcgtca tcggcgcgca accggggaac gcttttctgg cgaacgtttt gctgatcaaa
     8281 gataaccggg ttgaggtttt catcgaagcg aacggtaaac tgccggggac caaaatcaaa
     8341 actcaacgcc tgcggatcat caaggcccag ggttgggact aatctgtccg ctagctcttc
     8401 ctggcttagc ccgcgatttt ctgcgaccac ctgaagaaat tccgccgcgt tatcacgtaa
     8461 ggggcgggat ttagcccgtt gcgatatatg atgcagctgt atcagcgcca tatcattatt
     8521 catcagggta aggatgttca ggccgctgac agcacgggca gatttgcctt cttgtggcca
     8581 ggcgaggatc tgtgtcgtca gatcccgtgc cgtgctttcg tcaccaaaga gacttaacgc
     8641 cagaaatgcc cagttgtctt ttgcgggggc accagcttgt tgccacgcag tgaagagatc
     8701 ccaggcaaaa gctgccagcg tttgtggctg gcagaacgtt ttcagttgtt ccagcccgct
     8761 ataaaaacgt cccccctggg taaagcgcag catttcgccg ataatttcca gagcgtcacc
     8821 ggtaacaggc tgattattag tgataagtcg aggcctggac cacattgccg gatgccagaa
     8881 atcaggggct tttggaatgc gtgccgggta aatgtccatt ggaccctgtt taagcaactg
     8941 ctccagggca gaccatacat ctgtacgctg ccagcggttt gccacggttt gtagcaattc
     9001 gccatgtccc tgttcgtaca gtaaacgcag ggcaagtaat gcggcttcgc tattatcgct
     9061 gggtttggta aagacaagag ggataagtgc actagccgta tgttccggcc attgtaaaat
     9121 ccactggcga gccagatcac gctgcgccgc gaaacgtcgc cagacgcggg caacaggcag
     9181 cgccagttct gttgcgccaa aatttaaaat taacggaaat gtttcttttg gacgatgtga
     9241 aaaagccaac agcagacctg gtagcgcatc caatccgagg atactgagaa aatagtcctc
     9301 acccgcgaac agatgctttt tctcaatgat acgcagccag aaggtcaaag ccatctcacg
     9361 cggcaactgt gccaataaat acaggttcca gacactcttt tccggacgat aacgtgagaa
     9421 gttgtcattt tcccatatgt taattaacgc ttctatgtga ccgctgcgga gcgcctgttc
     9481 tgctaattgt gcttgcgggg agttccagcc attcaggatc tcttcaaccc gcccctggta
     9541 gtaatgccac tgaacaatga gtgaattgcg tgcagcatcc agttttccgg ggagtatata
     9601 attcagaaac agccatacat catccccaaa acccagttcc tgtaatacaa ctcgtccact
     9661 tttttgttcc atgcgttccg caagagattc cgtgctgaaa taacgggcag actcagtggc
     9721 ggcaagcctc tcttgttcgc ctggttgcca gtaaatttgc ggagttaatt cgagcggtgc
     9781 caaatcaaga cgtggagctg tcattttttt cttactacgc cacggtggtg aaacgagcat
     9841 ttcaggcaga ctgctgttat cggtgctatc aataaccggt tttggcggtg ccgataaata
     9901 actattcagc actgccctgg cttgagtact taaccacggc atcacccttt ctgctagttg
     9961 tggctgcgat gctatcagcg tttgtaacag cgtacgccag tattcatcgc gtgctttttg
    10021 ctgtgcaggc agctttttgt cttcgatgat tggataacct gggcgggctg gtggttcttt
    10081 taacgccagc agttctgcca gtgcggcgag tgtcgcatgg gggaagtttt tactgtattt
    10141 agccacacgt tgcaggctgg gtttgttttt atcagcaact aacagcaagg tgcggatgac
    10201 ttgcggatgg ttaatttgta ccagcagact gccgcaatct tctttatggg catatatcgc
    10261 gaggcgcgga acggctgcca gtccttgttc gcgcaaaagg gcggcgcagg ccgcgtagcc
    10321 gaagtggttt tcatgcgaca tgtagctggc ttctcgatcg ctaaatacgt ccaggcccca
    10381 gcatcgttcg agttttttta ccgccgtatt atcagtagca atgaccttta accactcctt
    10441 tgaatggaga cttgaccggg agctttcaag actggcgagt tcattggcta tttcaggttt
    10501 ttccgggagg agtagtgcaa taaaaggacg gcgaattttg ggtattcctg gcagtgcggc
    10561 aatgagtttg tcggcgcaac gctgccagct gctttcctct gccagagaga gatgcttacg
    10621 cagtcggaga tcgaattcgt tataggtttc tgagcgataa ccatagccat aatcctcgtc
    10681 ctgatactgc aatgtcacta gcagaggatc actctcataa catcgtgtaa taaactggcg
    10741 tgcaatcacc acctcagtcg catattccag tccctcttgt tggacgattt cgtcgagcaa
    10801 ctgttggctg tcattttgcg cgatacagac tagcgccgcc attgtccggg ccgggattga
    10861 gggtttgtgt tcgccgatca agttgatggc ctgcgtaatg gcggactgcc actccagtga
    10921 aaaattctca taacgccaga caatgttttt tttcttctca gctttttgta gcaattcatc
    10981 acccagtgtc tgaatgatgc tttcagtgat aacaggcact ggttcgcccg gccaattgcg
    11041 atggctgagt ggtgttttgc cttttttata tttcagttcc agttgggcgt tatccgccag
    11101 ccacggtaat tccttgtcca tgctctttcc ttattcaaac agggcttcaa tatcgttaat
    11161 cagctcgctg gcggtgatgg catcgagtac ggagaactgc gcttcctgtg ttgaattact
    11221 cccccagcca tagctttctg ctttgccttc ccatagccac agcttgctta agagttgctc
    11281 ggcgctaagt tcggctggcg acataccaac tgcaaagcct tcatcgattt ccatgattaa
    11341 cgaccagcgg cccagaggtt tgatcatcca gccgatccag ccaccatcct gcggttcacc
    11401 acgcacccaa cctttattcg ccagtcccat gacccgaccg ctcgggcatt ttctgcctgc
    11461 ccagcgggtc agttcactgg cattgcgctc ggcttctgtc agggcgtagc tgttacggtc
    11521 gagctggcgg aacggtggta gcagttcgta gtcggcaaaa agctgaccaa aggcggcagc
    11581 atccgttggt gatatttcca gaacgtgagg agtgccgata gagatatcgc cttccggcag
    11641 ggtgaaaaga tcatcgtcag cggtgctgta gctgttatct tctgctacgc gaaagcaagc
    11701 cagtagctgg ttttcggcgc tataaacgcc ccaaatcaga cggcgggtta agtgacgaac
    11761 cagcggatgc tcaaccagga agagctggaa gttttccagc gaccagcggc ggcgcaggca
    11821 catggcggat tccagccttg ctacctgctg ggcggcgatg gtacgcgcat cttttttcag
    11881 caatttgtag cggttaaccg catcgttcgc ccgcgtttca tcatcgcttt tgttcggttt
    11941 gggcaggtct ttcaggcggc tgccggaaac atcacgcaca aacggtttta aggtttcatc
    12001 aaagctgacg gtgaactgcc gtgggccaaa atccagcagc agcgaaccgt tatcatccag
    12061 accgagatcc ggtgctaacc gatcttcaag ctccgccacc gtgagttcgc ggctctcggc
    12121 aatgtcggca attttttctt ttgcccgctc ctgtaatgct ttgaatttca gtttctgggc
    12181 gatgccgtta agctgcataa gggcgatatc actaccgata gcagcgagaa tatccaaccc
    12241 aacggtggcg cgtttatgct gggattcacc aggccaggcg cgtattaatg gcgtcagttt
    12301 gcgggcggtg tcatcgttac cgagaacgcc taacgcagtg aacgcccaac tctctttcga
    12361 cggcgcgcca gcggtctgcc aggcggtaaa cagatcccag gcaaatcccg ccagtgagtc
    12421 ggcggaacag acgtctttca cctgcaataa tcccggatac agagcctctt cctgagggaa
    12481 gcggagcatt tcaccgaggt ggaggagggc gctatctggc agtgattggg catttgcttt
    12541 taataccggg cgcgtccaga gcgatggctg ataaaaggcc ggaagagtgg ggattttgtc
    12601 gggtgattat ctaagggatc gagcgcaagc agagcgttca ccgcatcggt tacttccggc
    12661 tggttataac gtcgggcgat ttcttgcagt aatggctgat gaccgttttc ggtaagcata
    12721 cgcaaggcag cgcgggcgtt atcctgtgct tcaccggctt tgccgagcgc cgcaggcagc
    12781 aggccggtaa gggcatgttc cgggtatttc aacagccagc tacgggcgtt ttcgcgtagc
    12841 gttttcagtt tgttgaaggc acgggcgacg gcaggagcca gctcactcgc tgcgaagtaa
    12901 ttcgtgatgg gcaaggcttc ttgtggatag cgtgcaagtg agtttactaa cccaggtaac
    12961 cctttgagtc caaaatgtaa catggcgtat tcggtatcgg tatgtggaga tagggtaagg
    13021 gcattccatg cttttactgc tttctccagc ggcaatgttg tcaaaatccc caactgccaa
    13081 taatgcccgg taggtgaatg aaacacctta aattcactta gtaaggttga ataatcttca
    13141 tttagccatg cctcaactac cgcttcggga gctttaatat atgtaccagc gatccgtcgt
    13201 tgaaaaccca tccttgccaa caggttggta gtactttctt catggctaac cgtgatgtgt
    13261 tttgaatacc agatatattt cgccaaaagt tgattactga tttcttctgt cagataacag
    13321 attggctcaa tgcctaatgg cgctaaatcc agcaccggaa tcggcgattt tttctttttc
    13381 gaaagccagg gaggggagac tactacggct ggcagtagat cggcgctggc atggtttgag
    13441 ggctgtgtca gttgttgctg gcatgatttc agtacggcaa ctgcgggtgt cgagagccag
    13501 ggaatgacct gttctgccag tgctggttgt gagataagca ttgtcattag cataatgcgc
    13561 caactgttct cttctttttg cccaagaagt tccgtcagtg ccgccattgc tgcatgtggg
    13621 aacgcagcgc aggctttcgt catccgatcg tgacagcgtt tagtatgccc ggctacacgt
    13681 ataagcagtg ttaatgcgaa cggatgattg atatgacgca acacatcggc gcagtagtca
    13741 cttgcggcat agggagcaaa tcggggcagg gctgcaacac cttgctcctg taatacggtg
    13801 gctgaccata tcgcaccaca atagtaatca tcaaaaatgg catatggctc gtgatatttt
    13861 tctaatgagg caagaatgtg ctcatcagtc gccactattt ttaaccactc aagcgagggt
    13921 aatttttttt gccccagtag acgctgggca atttcatgtg caatttctgg tttttcgggt
    13981 aataacaaag caattaatgg ttgccgccat tctggaatat gtggaattgc ggcaataagt
    14041 ttttgtgcac atttttgcca gagagactct tccgccagtg ataaatgttt acgtaggcgt
    14101 aattcaaatc tggaatattg ctccaagtag tcaggtgcaa tgccagacgg cagaataata
    14161 atgacattat tcgcataatc ccatttaata tcaatctgtt gaaggtgtat tattacatca
    14221 gtagcatatt ccagaccgcc ttcctggacg atctgatcca taacatcctc acgcacttta
    14281 aatcttgtgc atttcatttc aagtgccgtg agtacagcca ttactggtga tggtagctgt
    14341 gttgttttca ggccgtcgat ctgactgatc gcctgggcga cggtgttttg ccattctaaa
    14401 gaacaggcgc tgaagtcaaa tgtggctgtt ttattatccc gttgcgacac actacaggct
    14461 aagcgacgta attgggtcaa tttgtctgct tgctgaacag gaactccggg aaagcggcga
    14521 tgacttaacg tggtgtgtac aacttcgggc ggaaaaacga cagcaatatc tttcgccagc
    14581 cacggtaaaa cagcatctgt atttttgtca ggcgcacggg tttctgctac gaaagagggt
    14641 tgttcctgaa taatggtgga taagtccgct accttgagag agcccgttac cgtttgggaa
    14701 ggttgcaaag aattatcctt cgcttgctcc acatacccct tcttcacctt ctccgcaatc
    14761 agcttaagct ccgcttttgc cactgccgca gcatctgaaa aactttttat ctggctttgc
    14821 cctttggtgc caacttttcc ccaactgata tgcaactcgt ttccctgttg ctcaactgcc
    14881 cagaatttat gtgatttttc gtcctgatag ataaagtgtc tcattctgca tccctgtaat
    14941 tttatccagt tgaatgcaga tgctaccagt atttatgcgg gttagagaga gacaaaatcg
    15001 caagaaaaca actttaggaa tttataaaga aaaggcgctg tcgatgcagc gccttaaagg
    15061 gggattattt cacctgatgg cctggtttag caccagcatc cgggcttaac aggaagatat
    15121 ctttcccgcc aggaccagca gccattacca tgccttcgga gataccaaag cgcattttac
    15181 gcggtgccag gttggccacc ataatggtgt gacgcccgat cagtgcctgc ggatccgggt
    15241 aggcagaacg aataccggag aagacattgc gtttttcacc gccgagatcc agtgtcaggc
    15301 gcagcagttt gtcagaacct tcaacaaact ctgcgttttc gatcagcgcc acgcgcaggt
    15361 caacttttgc gaagtcgtca aaggtgatgg tttcctgaat cggatcatct gccagtgggc
    15421 cagtaactgg cgcggcagct gctttcactt cttctttaga cgcttccacc agtgcttcaa
    15481 cctgcttcat atcgatgcgg ttatacagcg ctttgaacgg attcactttg tggcccagca
    15541 gcggttgctg gataccatcc caggtcagtt ctgtattgag gaatgcttct gcgcgctcgg
    15601 tcagtttcgg cagtaccggc ttcaggtaag tcatcagcac gcggaacagg tttatgccca
    15661 ttgagcaaat tgcctgcagg tcggcatcgc ggccttcctg ttttgccacc acccacggag
    15721 cctgctcgtc gacatagcgg ttagccagat cagccagcgc catgatttcg cgcactgctt
    15781 taccaaattc gcggctttcc cacgcttcac caatcacttc agcggcatca gtgaaggttt
    15841 tgtacaactg cgggtcagcc aattcgcttg ccagcacgcc gtcaaaacgc ttgttgataa
    15901 agcccgcatt acgagaggcc aggttaacca ctttgttcac gatatcggca ttcacacgct
    15961 gaacgaaatc ctccaggttg agatcgatat catcaatgcg cgaagagagt ttcgcagtgt
    16021 agtagtaacg caggctgtca gcgtcaaaat gattcagcca ggtgctggct ttaataaagg
    16081 tgccgcgaga cttggacatc tttgcgccgt tcaccgtcac atagccatga acaaacaggt
    16141 tggtcggctt gcggaagttg ctgccttcca gcatggcagg ccagaacagg ctgtggaagt
    16201 aaacaatatc tttaccgatg aagtggtaca gctcggcggt ggagtctttc ttccagtatt
    16261 catcgaagct tacgctgtcg ccgcgcttgt cgcacagatt cttgaaagaa cccatgtagc
    16321 caatcggtgc gtccagccag acgtagaaat atttgcccgg cgcgttcgga atttcaaaac
    16381 caaagtatgg cgcgtcgcgg gagatatccc actgttgcag gccggattca aaccactcct
    16441 gcattttatt tgccacctgc tcctgcaacg caccgctgcg ggtccatgcc tgcaacattt
    16501 cgctgaaaga gggcagatca aagaagaagt gttcagaatc acgcattacc ggcgtagcgc
    16561 cagaaaccac cgatttcggc tcgatcaatt cagtcgggct gtaggtcgcg ccgcagactt
    16621 cgcagttatc gccgtattga tccggggatt tacatttcgg gcaggtgcct ttcacaaaac
    16681 ggtccggcag gaacatgcct ttttccggat cgtacagctg agagatggtg cggtttttaa
    16741 taaaaccgtt ctctttcagg cgagagtaga taagttctga caactggcgg ttctcttcgc
    16801 tgtgcgtcga gtgatagttg tcatagctga tgttaaagcc tgcgaaatca gtctgatgct
    16861 cctgactcat ttcgccaatc atctgctccg gggtgatacc aagctgctga gctttcagca
    16921 tgatcggtgt accgtgggca tcgtcggcgc agatgaagtt gacctcgtgg ccgcgcattc
    16981 gctggtaacg gacccagaca tcagcctgga tgtgctccag catatggccg aggtggattg
    17041 agccgttagc gtacggcagt gcgcacgtca ccagaatttt cttcgcgact tgagtcatag
    17101 taggcattac ttcttaatag tgaaaagggg ttagatagta ccaaatggga aaatgttaag
    17161 taagtacttc tctatattct tcaggtaaac tatcagctga tggtttatct atacaaaaaa
    17221 ggagtcggga tgaacgaaca atcccaggcc aaatcaccag aagccttgcg cgcaatggtc
    17281 gccgggacac tggccaattt tcagcaccca accctgaagc ataacctcac cacgctgaaa
    17341 gcgttgcacc atgtcgcctg gatggacgac acgttgcatg tggaactggt tatgccattc
    17401 gtctggcata gcgcgtttga agagttaaaa gagcaatgta gcgccgaact gctgcgtatc
    17461 accggggcga aagctatcga ctggaaactg tcgcacaata tcgccacctt aaaacgcgtg
    17521 aagaatcagc cgggtattaa tggtgtgaag aacatcattg cggtcagctc cggtaaaggc
    17581 ggcgtgggta aatcctccac ggcggtaaac ctggcgctgg cactggcggc ggaaggggcg
    17641 aaagtcggca ttctggacgc cgatatctac ggcccatcaa ttcccaccat gctgggcgca
    17701 gaaaatcagc gtccaacgtc gccggacggt acccacatgg caccgattat gtctcatggc
    17761 ctggcaacca attcaattgg ttatctggtc actgacgaca atgcgatggt gtggcgtgga
    17821 ccgatggcga gcaaggcgtt aatgcagatg ttgcaggaaa ccttgtggcc ggatctcgac
    17881 tatctggtgc ttgatatgcc gccaggcacc ggtgacattc aattgacgct ggcgcaaaac
    17941 attccagtaa ctggtgcagt cgtggtcacc accccgcagg acatcgcgtt gatcgatgcg
    18001 aaaaaaggca ttgtgatgtt cgagaaagtc gaggttccgg tgcttggtat tgtcgagaac
    18061 atgagtgtgc atatttgcag taactgtggt caccatgaac cgattttcgg taccggtggt
    18121 gctgagaaac tggctgaaaa ataccacaca cagctgctgg ggcagatgcc gttacacatc
    18181 tccttacgtg aggatctcga taaaggcacg ccaacggtta tcagccgtcc ggagagcgaa
    18241 tttaccgcta tctaccgcca gctggctgac cgcgttgcag ctcagctcta ctggcagggt
    18301 gaagtcattc caggcgagat ttccttccgc gcggtctaac gcctattaac cattctggtt
    18361 attcttcata caccgcagag atgttacatt gatgcggtgt attagttttt gccgcattaa
    18421 tgaacactta tttaatactg cgaatattct gcggaccttc gtccgaaact ctacaaagtc
    18481 gattgatcat acaggcaatg cttcattatc atccttcgtg gctgtgtagt ggatgtacag
    18541 ctatagtttg ttatgcactc attctatcca ggcataacct ttactacagg gataacgttc
    18601 aatgaataag tattggctgt caggaattat ttttcttgcc tatggattgg cttctcccgc
    18661 attctcttca gagactgcca cattagcgat taatggcagg atcagtcccc caacatgcag
    18721 tatggctatg gttaatggtc aacctcaaca gcattgtggt cagttaacgt ataatgttga
    18781 cacccgtcat ctgttttctt cgcccgtcaa aggtgtaacg acagaagtgg ttgttgcagg
    18841 tagcgatagc aaacgtcgaa tcgtgttaaa tcgctacgat taatttggca acctgatttt
    18901 aattgcattt agaaagccg
//
LOCUS       DAZFVO020000096        18522 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFVO020000096 DAZFVO020000000
VERSION     DAZFVO020000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.097"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            81..1301
                     /locus_tag="ACHKNB_RS15685"
                     /old_locus_tag="pgaptmp_003137"
     CDS             81..1301
                     /locus_tag="ACHKNB_RS15685"
                     /old_locus_tag="pgaptmp_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4196600.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARRMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1274..1903
                     /locus_tag="ACHKNB_RS15690"
                     /old_locus_tag="pgaptmp_003138"
     CDS             1274..1903
                     /locus_tag="ACHKNB_RS15690"
                     /old_locus_tag="pgaptmp_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4196601.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1904..3064)
                     /gene="ybdL"
                     /locus_tag="ACHKNB_RS15695"
                     /old_locus_tag="pgaptmp_003139"
     CDS             complement(1904..3064)
                     /gene="ybdL"
                     /locus_tag="ACHKNB_RS15695"
                     /old_locus_tag="pgaptmp_003139"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HGU4196602.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3172..4260
                     /gene="hcxA"
                     /locus_tag="ACHKNB_RS15700"
                     /old_locus_tag="pgaptmp_003140"
     CDS             3172..4260
                     /gene="hcxA"
                     /locus_tag="ACHKNB_RS15700"
                     /old_locus_tag="pgaptmp_003140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HGU4196603.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4270..4467)
                     /locus_tag="ACHKNB_RS15705"
                     /old_locus_tag="pgaptmp_003141"
     CDS             complement(4270..4467)
                     /locus_tag="ACHKNB_RS15705"
                     /old_locus_tag="pgaptmp_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HGU4196604.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4643..6748)
                     /gene="cstA"
                     /locus_tag="ACHKNB_RS15710"
                     /old_locus_tag="pgaptmp_003142"
     CDS             complement(4643..6748)
                     /gene="cstA"
                     /locus_tag="ACHKNB_RS15710"
                     /old_locus_tag="pgaptmp_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HGU4196605.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6929..7342)
                     /gene="entH"
                     /locus_tag="ACHKNB_RS15715"
                     /old_locus_tag="pgaptmp_003143"
     CDS             complement(6929..7342)
                     /gene="entH"
                     /locus_tag="ACHKNB_RS15715"
                     /old_locus_tag="pgaptmp_003143"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HGU4196606.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7345..8091)
                     /gene="entA"
                     /locus_tag="ACHKNB_RS15720"
                     /old_locus_tag="pgaptmp_003144"
     CDS             complement(7345..8091)
                     /gene="entA"
                     /locus_tag="ACHKNB_RS15720"
                     /old_locus_tag="pgaptmp_003144"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HGU4196607.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8091..8948)
                     /gene="entB"
                     /locus_tag="ACHKNB_RS15725"
                     /old_locus_tag="pgaptmp_003145"
     CDS             complement(8091..8948)
                     /gene="entB"
                     /locus_tag="ACHKNB_RS15725"
                     /old_locus_tag="pgaptmp_003145"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HGU4196608.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8962..10572)
                     /gene="entE"
                     /locus_tag="ACHKNB_RS15730"
                     /old_locus_tag="pgaptmp_003146"
     CDS             complement(8962..10572)
                     /gene="entE"
                     /locus_tag="ACHKNB_RS15730"
                     /old_locus_tag="pgaptmp_003146"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HGU4196609.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10582..11757)
                     /gene="entC"
                     /locus_tag="ACHKNB_RS15735"
                     /old_locus_tag="pgaptmp_003147"
     CDS             complement(10582..11757)
                     /gene="entC"
                     /locus_tag="ACHKNB_RS15735"
                     /old_locus_tag="pgaptmp_003147"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HGU4196610.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12132..13088
                     /gene="fepB"
                     /locus_tag="ACHKNB_RS15740"
                     /old_locus_tag="pgaptmp_003148"
     CDS             12132..13088
                     /gene="fepB"
                     /locus_tag="ACHKNB_RS15740"
                     /old_locus_tag="pgaptmp_003148"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HGU4196611.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAAQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13092..14342)
                     /gene="entS"
                     /locus_tag="ACHKNB_RS15745"
                     /old_locus_tag="pgaptmp_003149"
     CDS             complement(13092..14342)
                     /gene="entS"
                     /locus_tag="ACHKNB_RS15745"
                     /old_locus_tag="pgaptmp_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HGU4196612.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14453..15457
                     /gene="fepD"
                     /locus_tag="ACHKNB_RS15750"
                     /old_locus_tag="pgaptmp_003150"
     CDS             14453..15457
                     /gene="fepD"
                     /locus_tag="ACHKNB_RS15750"
                     /old_locus_tag="pgaptmp_003150"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HGU4196613.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15454..16446
                     /gene="fepG"
                     /locus_tag="ACHKNB_RS15755"
                     /old_locus_tag="pgaptmp_003151"
     CDS             15454..16446
                     /gene="fepG"
                     /locus_tag="ACHKNB_RS15755"
                     /old_locus_tag="pgaptmp_003151"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HGU4196614.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16443..17258
                     /gene="fepC"
                     /locus_tag="ACHKNB_RS15760"
                     /old_locus_tag="pgaptmp_003152"
     CDS             16443..17258
                     /gene="fepC"
                     /locus_tag="ACHKNB_RS15760"
                     /old_locus_tag="pgaptmp_003152"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4196615.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEKAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17255..18388)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKNB_RS15765"
                     /old_locus_tag="pgaptmp_003153"
     CDS             complement(17255..18388)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKNB_RS15765"
                     /old_locus_tag="pgaptmp_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HGU4196616.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4350 a         5242 c         4786 g         4144 t
ORIGIN      
        1 actctgcttt gcagtgttta ctttataatt aacagactta tctcactgat caccctgtaa
       61 cgttcagaga gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg
      121 aagctgcacg agagcgtatt acctggacac tcaacaccct accccgcgtt tgtgtctcat
      181 tttctggcgg caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacgaa
      241 tgggcaaaaa aatctgcgtt ttgtttatcg actgggaggc gcaattctct tgcaccatta
      301 actatgttca gtccctgcgc gagttgtaca ccgatgtcat cgaagagttt tactgggttg
      361 cgctcccgct tacgacgcaa aattcccttt cacaatacca acccgaatgg cagtgctggg
      421 aacctgatgt cgaatgggtg cgtcagcccc cgcaagatgc gataaccgat cctgactttt
      481 tctgctttta ccagcctggc atgaccttcg aacaatttgt acgtgagttt gccgaatggt
      541 tttcacaaaa acgtccggcg gcgatgatga tcggcatccg tgcggatgag tcctacaacc
      601 gttttgtcgc catcgccagt ttaaataaac aacgttttgc cgacgataaa ccatggacta
      661 cggccgcacc aggcggtcat agctggtaca tttaccccat ttacgactgg aaagtggctg
      721 atatctggac ctggtatgca aatcatcaaa gtctctgcaa cccactgtat aacctaatgt
      781 atcaggcagg cgttcctctg cgtcatatgc gaatttgcga accttttggc ccggagcaac
      841 gacaaggatt atggctctat cacgttatcg aaccggatcg ctgggcagct atgtgcgcac
      901 gagtcagcgg ggtaaaaagt ggcggcattt acgccggaca tgacaatcat ttctatgggc
      961 atcggaaaat cctcaagcca gaacatttag actggcaaga atatgcatta ttgctgctca
     1021 atagcatgcc ggaaaaaaca gctgagcatt accgcaataa aatcgccatt tatttgcact
     1081 ggtatcagaa aaaaggcatc gaggttccac aaacccagca aggggacatt ggcgcgaaag
     1141 atatcccctc ctggcggcgg atatgcaaag ttttactcaa taacgattac tggtgtcgag
     1201 cattatcatt tagtcctacg aaagcgaaga actatcagcg ttataacgaa cggataaaag
     1261 gaaaacgtca ggaatggggg atactatgca acaacgatta acgcaggatt taacgcaatt
     1321 tctcgccagc ttgccggaag atgatcgcat caaagcgatt aatgaaatcc gcatggcgat
     1381 ccatcaggtt agcccctttc gtgaagagcc agtggattgc gttctgtggg ttaaaaacag
     1441 ccaacttatg cctaatgatt acaacccaaa caacgtggca ccacccgaaa aaaaactgct
     1501 gaaaaaatcc attgaaatag atggttttac ccaacccatt gtggtcacgc atacggataa
     1561 aaatgccctg gaaatcgtgg atggttttca ccgccatgaa attggtaaag gctcatcatc
     1621 attaaaatta cgcttaaaag gttatttgcc ggtgacctgt ctggaaggta cgcgtaatca
     1681 acgcatcgcc gcgactattc gtcataatcg cgctcgtgga cgccatcaaa tcaccgccat
     1741 gtcggaaatc gtccgtgaac ttagccagtt gggatgggac gataataaaa ttggcaaaga
     1801 gctaggaatg gacagcgacg aagtgctgcg cctgaagcaa attaacggcc tgcaagagct
     1861 gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa taactaaagc tggcgcaggc
     1921 gttcagctgc tgccagcaac gtcgattcct tcttggcaaa acagagacga atcagtttat
     1981 gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc cgctacgccg tgctcctgcg
     2041 tcagccactg gcaaaactca acatcatcca gggtagaaac ctcgctgtaa tccaccagca
     2101 aaaagtatgt accttcacac ggtaaaatct ccagccggct ttcatttaaa gcattcacca
     2161 gaatatcgcg cttctggcga taaaagtccg gtaacgcaag ataatgctca ggttctgcac
     2221 gtagcatatc agcaagcgcc agctgtgccg gggtattcac cgaaaaggtc agatactgat
     2281 gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac acaataaccc actttccagc
     2341 cggtcatatg ataggtcttg ccaaatgaag aaaccgccac tgcccgctca cgcagctgcg
     2401 gatgcgccag cacactggca tggccctgtt gtgaaaagtt gatgtgctcg tagacttcat
     2461 cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca caaagcggcg aaatcagcct
     2521 gctgccagac agttgcactg gggttatgcg gagtgttgag gatcaccagt ctggtgcgct
     2581 cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa atgcggtggt tgcagtgcca
     2641 tacgcttcac tattccccca gaaagcgcga tggcggggga gtaactgtca tagctgggat
     2701 caaaacaaat cacttcatcg ccattgcgca ccagtgcggt aatcgccgcg tataacgctt
     2761 ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc tggttgatag ccatacaaac
     2821 gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac gccggtcatg ggcgcgtatt
     2881 ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg taaatagcgc ggaccatcaa
     2941 aatcaggaaa gccttgcgac aggttaatcg cctggtgttg ctgcgccagc gcgctcatct
     3001 gggtgaaaat agtggtgcca agttgtggaa gtttgctttg tggaatcaga gggttatttg
     3061 tcattttatt gtacctgacg aagttgcgtg ttgaaggcac tataacacga tgctagtatt
     3121 tggcaatcaa gacgtttaga tgtctaaata aaacaataag gacaacacaa catgcctcac
     3181 aatcctatcc gcgtggtcgt cggcccggct aactactttt cacatccagg aagtttcaat
     3241 cacctgcacg attttttcac tgatgaacaa ctttctcgcg cggtgtggat ctacggcaaa
     3301 cgcgccattg ctgcggcgca aaccaaactt ccgccagcgt ttggacttcc aggggcaaag
     3361 catattttgt ttcgtggtca ttgcagcgaa agcgatgtac agcaactggc ggctgagtcc
     3421 ggtgacgatc gcagcgtggt gattggcgtc ggcggcggcg cactgctcga caccgcgaaa
     3481 gccctcgccc gccgtctcgg tctgccgttt gttgccgttc cgacgatcgc cgccacctgc
     3541 gccgcctgga caccgctctc cgtctggtat aacgatgccg gacaggcgct gcattatgag
     3601 attttcgacg acgccaattt tatggtgctg gtggaaccgg agattatcct caatgcaccg
     3661 caacaatatc tgctggcggg gatcggtgac acgctggcga aatggtatga agcggtggtg
     3721 ctggctccgc aaccagaaac gttgccgcta accgtgcgac tggggatcaa taatgcgcag
     3781 gccattcgcg acgtattgtt aaacagtagc gaacaggcgc tgagcgatca gcaaaatcaa
     3841 cagttaacgc aatcattttg cgatgtggtg gatgctatta ttgctggtgg tgggatggtc
     3901 ggtggtctgg gcgatcgttt tacgcgtgtg gcggcggctc atgccgtgca taacggtctg
     3961 actgtcctgc cgcaaaccga gaaatttctc cacggcacca aagtcgctta tggcattctg
     4021 gtacaaagcg ccttgctggg tcaggatgat gtgctggcgc aattaaccgg ggcgtatcag
     4081 cgttttcatc tgccgaccac gctggcggag ctggaagtgg atatcaataa tcatgcggag
     4141 atcgacaaag tgattaccca caccctgcgt ccggtggaat ccattcatta cctgccggtc
     4201 acgctgacac cagatacgtt acgtgcagcg ttcgaaaaag tggaatcgtt taaagcctga
     4261 cgtacatatt tagcagcagc gcgcgccgcc ttttccaccg tagcgcgcgt cctgccgctc
     4321 acggaaaaac tcctcgtagg tcatcggcgt ttgatcggga tggttaaccc gcatatgttc
     4381 gacatagttg tcgtaatcag gcatgccaat catcagcttc gccgcctgac ctaaatattt
     4441 tccggctttt gccagtgaat caaacatctt ggcctccgct aaccatgttg ctgaaagagt
     4501 gccggatgcg gcgtgaacgc cttatccggc ctacaaatgc tatcaacgcg ctcggtcagt
     4561 cccctcgccc caccggggag agggtgaggg ggaaccctcg gcgccatgcc aacattcgcc
     4621 aacattcccc ctcactctga ctttagtgtg cgccttttgc ctgcgccacg atctcctcga
     4681 cattttccgg cattggctca tacggcgttt ctttcgccgt tggcttcggc tctttcaatg
     4741 ccgcaagtgc cgtcttaatc gagaacagtg ccagaaccac cacgaccacc ataaagaaga
     4801 tggttaaccc ggcatccaga cggttgttga acaccagttg cgccagctgt gactcagtat
     4861 actgcgacgg aatattgccg ctgtcgatca ttgcctggaa cttgttagca atggccagga
     4921 agccgacttt cgcatccggg ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca
     4981 gtagccaggc cgttggtacc aacgccaccc aggcgtaacg ttgacgtttc atcttgaaca
     5041 acaccacggc acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca
     5101 gcggccacag agtgttaata ccgcccagcg gatcgaccac gccctgatgg aggaagtagc
     5161 cccacgccag tacgcacagc gccgttgcca gcaggttagc aggcagtgaa tcggtccgct
     5221 tcaggccagg agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac
     5281 ccgcatctac cgccgtcaga ataaacagag cttcaaacag aatggcgaag tgataccaga
     5341 acgccacatc catcatacca cccagcgcac cgtgcaggat gtaagccatc cccaccgcca
     5401 gcgtcggcgc accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct
     5461 ggttgagcgt atctggtgta atgctaaagc cccagctact caccacctgc gcggcagaag
     5521 cgactacatc cgccgtccct gccggagcca gcaccgccat tgggctgttc atggcaaaat
     5581 acacgcccgg atcgatgata caggcagaaa ccagcgccat aatcgccacg aaggattcca
     5641 tcaacatccc accgtagccg ataaagcacg cctgcccttc gttcgccagc atcttcggcg
     5701 tggtgccgga agagatcagc gcatggaagc cagacaccgc accacaggcg atggtgataa
     5761 acaggaacgg gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttggtca
     5821 gcgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga ccgacgatag
     5881 tcccgatttt caggaaggtc gagagatagt cacgcggggc cagcagcaac cacaccggca
     5941 gcaccgccgc cacaaaaccg tagcccacca gcatccaggt cagctgcacg ccggtaaagt
     6001 caaagtacgg tgcccaggtc ggactttctg ccacccagcc gccagagata atggcgaaaa
     6061 tcaggaacac caggccaatc actgacactt cgccaatgcg gcccggacgc agatagcgca
     6121 ggtagattcc cataaaaatc gccagcggaa tggtgaacgc aacggtatac gttccccacg
     6181 ggctatgagt cagggctttc accacgatca tcgccagtac tgcaaggata atgaccatga
     6241 tcataaagca ggctaccagc gcaatcaccc cggcagttgg ccccatctct tctttgacca
     6301 gctcacccag cgatcgaccg tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca
     6361 ccgcaccagc gagaaccacc ccggcgagca gccagatcat ccctggcagg taacccattt
     6421 gcgccgccag taccggccca accaacggac ctgctccggc aatggccgca aaatggtgac
     6481 cgaacagcac tttcttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg
     6541 gcgtcatacg cgtcgggtca accgccagca catttttggc gatatacagc ccataaaaac
     6601 ggtaagcgat cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt
     6661 ccccacgatt taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc
     6721 agacgaggta tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagagatg
     6781 ttacattttg tttctataac atctttgtac gatttaacaa cacggtaacc atgtaaatcg
     6841 gagttgaaac caatatttaa cttaacaatg ttaactcgat cactccgtta accgagtttt
     6901 cctgcaatct catttaattc tgtcgggctc atcccaaaac tgccgtaccc agccgacaag
     6961 tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc
     7021 caagatgcag cggctggcag acgccgcgta ccttgccttc agacaccggg cgatgatggg
     7081 tggcgttaag ttccgtaccc accacacact gcccgtcacg ggtcatcata aatccggcca
     7141 tcgatcccag cgtttcagcc agcgccgccg acgcgccgcc atgcagcagg ccaaacggct
     7201 gatgagtacg ggtatcaacc ggcatttcgg cttccagcac atcatctccc agacgggtat
     7261 acacaattcc cagatgcgcc accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg
     7321 ttaaatggcg tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat
     7381 atcctgtagg gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat
     7441 ctcttgtgga cgggcgattt tccccagcgg aatgccgagt ttaaactgct cgccaaagcc
     7501 gcgaatacgc tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc
     7561 ggtggagcca ggcgaaacca cattacagcg cacgccgcta cccgccagtt ccagcccgac
     7621 gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca taagcactca taccaatacg
     7681 cggcgtgtgt gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct gacggcgaaa
     7741 ctggttcatg gtttgctgga acaggttaaa cgcaccgccg acgttaaccg caaaagtctg
     7801 ctgccagtcc tctttactga gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt
     7861 gatcagcacg tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgccacctg
     7921 cccggcgtcg gcaacatcca ttacttcggt cgcaaaggga tattgctcct gagtgaacgc
     7981 ttgatcaaaa cctgtaactt tcgctcccgc ctcaacaaac gccagcgccg tggcgtagcc
     8041 gataccttta cctgcgccgg ttacccagac atttttaccg ctgaaatcca ttatttcacc
     8101 tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt ttttcgccag catgacaaag
     8161 tcgatatcac catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc
     8221 agaccgtagt cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc cagcaacggc
     8281 aggataacct cacgcagcgc cgctttgctg gcggggacag gtgctggcag taattcttca
     8341 gtcatcacca cccggccaga ccgcccggcc acatatttca gcgacatcaa atgctcgtca
     8401 cggctgaaat cggccagcgc atccgccacc ataaacggtt taatatcgcg cataaatgcg
     8461 tcggttgcgg tggtcataca gccaatgtgg gcatataccc cggtaataat cagctggtta
     8521 cgtccactct ctttgagcat ttgctccagc ggagaacgat gaaacgcgct gtagcgccac
     8581 ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc
     8641 ggcgaacggg tgaggcccgg cccccacata tcattcaaca gcgcccgatc ttcatcgctc
     8701 tgctctttcg gctgggcggt gtaataaacc gggatattgt gctgtttgca gtagtcgcgc
     8761 agcgcagcaa tattcgcgat cacctgctcc atcatcgggc agttctcgcc ccagaagctg
     8821 acaaaatagt cctgcatatc atggattaac aacgcggcac gttgcggttc aaaggcccag
     8881 tcgactttat tcttagggat atcgtgagac tccggcagtg cgtaagcctg taattttgga
     8941 atagccatcg tgttctctcc ttcaggctga tgcgcgtgac gccagccact gacgtaattg
     9001 ttttttatcg actttcccga ccgccgtcag cggaagtgaa tctacacact ccacgcgatc
     9061 cggtaattta aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg
     9121 cagcggttct ttcaccacca gataagcgca gcttttttcg cccatcagct catcttccat
     9181 gctcaccagt gcggcgtaga tcaccgccgg gtggcgcagc agcaggtttt cgatctcttc
     9241 ggcagcgatc ttctcaccgc cacggttgat ctgatctttc tcgcgcccct gcacggtgat
     9301 gtacccctct ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa
     9361 ggcactggca ttgtgctgcg gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt
     9421 catcaggcgt ccaacttccc cttgcggcag tggatttcct tcagcatcag caacccatac
     9481 ttcatcgtcc ggacacattg ggtaaccctg ggtatggata attttctccg cgctatcatc
     9541 aagtcgggtg tagttcacca gcccttccgc catgccaaac acctgctgca acaggcagcc
     9601 aatctcagcg ggaatacgcg ccgcaagggt ggctgaaaga cgtgcgccgc cgacctgtaa
     9661 cagtttcagc gaggcaagct gcgcccggct ttcaccttcg gtcagcgcct gcaaccacag
     9721 gctgactgcc ggtggcacca gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa
     9781 gcaaagcgtg gcgctgggat cggccgccag aacaaccgtt cctccggcaa gaaagacgcc
     9841 cagcgatccc ggcgaactca tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg
     9901 tgtctgttgt gtgaactgac aaatctcgac gctacgacgc acgctgtagt agtagtcgtt
     9961 atgagtgcgc gggatcagtt tcggtgtgcc ggtggtgccg ccggaaagct ggaaataggc
    10021 cacttcatca gcaggtgatg gcgtggcagt aaaatcctca gccggatggt taatcgcatc
    10081 ctgcaagtta tgctcaccgc tgtcgttgtg cagttgcacc acgcgaatgg aggaatgttc
    10141 tgtgacgaaa gtattgagga aatcatcccc gctaaacagc gcatgttggc gatcggcaat
    10201 cagcaatgcg ggttcaatct ggctggcata ggcgttcagt tcactacgct gatggctgaa
    10261 cagcgccagc accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa tatacaattc
    10321 agcgacgtta cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgtaa
    10381 actacacgcg aggttatccg ccgcctgatt cagctcccgg taactcaact gtcgctcgcc
    10441 gtcgataacc gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa
    10501 atcctgccag tagccttttt cccgatagcg acgggcaaac tcttccggcc agcgggtgaa
    10561 tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt
    10621 gacgcctgtt tcgcgccact cacccaacgg tgacgacgca ggcacaatcc ccgctccggc
    10681 aaacagacgc acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcgcc
    10741 gttaccttcg ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaatggctc
    10801 cagttcagca ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg cgggggttgg
    10861 atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg
    10921 agttgcgaga tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa
    10981 ctcactactg cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg
    11041 gcgatctttt tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg
    11101 ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag
    11161 cagcagttcc gggctggccc ccagcaggac gccaccatca gccagcggga catggaagtt
    11221 gtaactaacc gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc
    11281 gtcagtggtg atatcaatca atcgtgacaa caccactttg tcgacctgcg gcgtggcggt
    11341 aagtgcggcg gcgcgagcaa ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg
    11401 ttccaccacg ttcagcgact ggctgcgggt aaaacggcgg gctgaggtct gtttttcctg
    11461 gcgggagaac gactgccagg attcgggaat atacagcgac gaaggctgac gtggatcgaa
    11521 gggaatcgcc ccgaccatca ccggattttt gatgccctgc gctttggcat cggcaaacag
    11581 cgcggcgagt ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc
    11641 gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg
    11701 attgggcgca agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc
    11761 atcctccaca aaatgataaa ggctttaata atgattatca tttatatttt cggtcgctaa
    11821 cctaaaagca aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt
    11881 gcatctggtt gtggttacaa tgaaaatgag aagcattatt gatggattcg cataagcgca
    11941 atgtgatgtc ctgcgccgtt ctgccccctc tcccttccag ggtgagggct ggggtgaggg
    12001 ttaatgttcg caccagtgct ggctgttccc ctcaccctaa ccctctcccc aaaggggcga
    12061 ggggacggat tgtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag
    12121 gaagctgatt tgtgagactc gccccgctct accgcaacgc ccttctatta acaggacttt
    12181 tgctttcagg aatagccgca gctcaggccg ctgactggcc gcgtcagatt actgacagcc
    12241 gtggcacaca tacactggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga
    12301 ccggctcact gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata
    12361 accgcgtcgc ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca
    12421 aactgcaacg gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg
    12481 atctgatttt aattagcgca accggcgggg attcggcgct ggcactgtat gatcagcttt
    12541 ccaccatcgc cccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa
    12601 cgcagcttgg cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg
    12661 ataagcaact ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca
    12721 ttgtctatac cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc
    12781 agatgctgga acaactcggc tttacgctgg cgaagttgcc cgcagggtta aacgccagcc
    12841 aaagccaggg caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgctgggt
    12901 taaatggcga gtcactattc cttttcgccg gtgatcagaa agatgccgat gcgatttatg
    12961 ccaatccact gctcgcgcac ctgcccgcag tacaaaacaa gcaggtttat gcgctgggaa
    13021 ccgagacgtt ccgcctggat tactacagcg ccatgcaagt gctggaacga cttaaggcgc
    13081 tgttttaagc attaactgtc agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc
    13141 aactccacca gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg
    13201 cttgcggaag caaccggtgt catcatcgcg cccaaaccac ccagcagcgc cgcgcctatg
    13261 gcatcgcccg tcacgttctg cgccgtccac aaaccgttaa tccgccctaa catcgcttcc
    13321 ggggtttgcg tttgcagcat tgtatattgc agcaacgaac taaccgcgct cagccagccg
    13381 aacagcgcca gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg
    13441 gcgaggaacg atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc
    13501 agcttcccgc tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa
    13561 ccaatctgtg ccgctgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc
    13621 gcgctcgcca tcgtcaataa accacccagc agcgcaatcc ctcctaccag cgggctggcg
    13681 agcagaaaac gaaatcctgc cagtaatgat ttcaacggat gctcgcgcgg ctgcggtggc
    13741 ggtggcaacg ccggaaggct taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc
    13801 agcccgtagt tccaggctac gccgccggtc gccagcaata aaccgccaat catgggcgaa
    13861 atcaccgacc ccagacgcac ggtcaacatg gtgatcgccc cggcctgcat taagttttca
    13921 cgccctacca gtgcaggtgt tgccgccagt agcgccgtaa caccaagtga tgcgaaaaaa
    13981 ccatcccata aaccaagtaa atagattgcc agcaatgacg gctccggcag cagcgcgttc
    14041 aggcacagtc caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg
    14101 cgttcatagc gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg
    14161 gtcagcgtca ccgaaagccc cacctgccag gtagaatgcg tcatcatctg gatctgcacc
    14221 ggcaccgcga cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact
    14281 gcgcgaaacg ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc
    14341 attacaatgc cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca
    14401 tatccaaata agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg
    14461 ttctgttgcc gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc
    14521 gacggcatta agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc
    14581 cctctccggc acctgccaga gcgccgactg caccatcgtg ctcgacgcgc gactgccgcg
    14641 cacccttgcc ggtttacttg caggcggcgc gcttggcctt gccggggcgt tgatgcaaac
    14701 cctcacccga aatccacttg ccgaccccgg cttgcttggc gtgaatgccg gagccagctt
    14761 tgccattgtg ctgggtgcgg cgctgtttgg ttactcatcc gcgcaggaac aactggcgat
    14821 ggccttcgcc ggggcgctgg tggcctcatt gattgttgcc tttaccggca gtcagggcgg
    14881 cgggcagtta agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga
    14941 aggactgacc agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg
    15001 gcaagccggt tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt
    15061 tctgatcgcc ggagcaactg cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg
    15121 cagtgacacc gcgacggcgc tgggcagtcg cgtggcgcgc acacagttaa ttggtctgct
    15181 ggcgattacc gtgctttgtg gtagtgcgac agcggtagtt ggcccgattg cctttattgg
    15241 cctgatgatg ccgcatatgg cgcgttggct ggtgggtgcc gatcatcgct ggtcgctgcc
    15301 cgtcacgcta ctcgccaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat
    15361 tgtgcccggc gagttgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat
    15421 cttcctcgtg cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc
    15481 atcacctgtt tgctgcttat ttccgcctgt gtggttgcag gtatctgggg attacgcagc
    15541 ggtgccgtca cgctggaaac ctcgcaggta ttcgccgcgc tgatgggcga tgcgccgcgc
    15601 agtatgacga tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt
    15661 ggcgcggcac tgggcgtcag cggtgcgatt tttcagtcgc tgatgcgtaa cccgctcggc
    15721 agccctgacg taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg
    15781 ctgtttggtc aggacctgac ggctatcgcg ctggcagcaa tggtgggcgg cattgtcact
    15841 tctctgttgg tctggctgct cgcctggcgt aacggtatcg acacctttcg gttgattatt
    15901 atcggcatcg gcgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg
    15961 tctttagaaa cggcgctaac agcgggtttg tggaatgccg gatcgctcaa tggcctgacg
    16021 tgggcaaaaa cttcgccttc cgcacccatc attatattga tgctcattgc cgccgccttg
    16081 ctggtgcggc gaatgcgctt gctggagatg ggcgatgata ccgcctgcgc gctgggcgtc
    16141 agcgtcgaac gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca
    16201 acagcgcttg ccgggccgat ttcctttatt gctttagtcg cgccgcacat ttcccgacgc
    16261 attagcggca ccgctcgctg ggggctaacc caggcggcgc tatgcggggc gctgttactg
    16321 ctggcggccg atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc
    16381 gttaccgtca gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa
    16441 aaatgaccga atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat
    16501 ataccgttgc ggaaaatctg actgtagaaa ttcctgatgg tcacttcacg gcaattatcg
    16561 gaccaaatgg ctgtggtaaa tccacgttac tgcgtacctt aagccgcctg atgacgcctg
    16621 ctcacgggca tgtctggctg gatggcgagc acattcaaca ttacgccagt aaagaagttg
    16681 cacgccggat tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg
    16741 agctggtggc gcgtggacgt tatccgcatc aaccgctgtt tacccgctgg cgcaaagagg
    16801 atgaaaaagc ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg gcagatcaaa
    16861 gcgtggatac cctttctggc ggacaacgcc agcgagcgtg gatcgcgatg gtgctggccc
    16921 aggaaacagc aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga
    16981 ttgatctact ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg
    17041 tgctgcacga tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag
    17101 ggaaaattgt tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca
    17161 tttatggtct gcgatgcatg atcattgacg atccggtagc cggaacgccg cttgtggtgc
    17221 cgctcggacg aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg
    17281 catcctgttt tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc
    17341 cgccgatcag cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg
    17401 gcaatgacgg gcttaactga tatttaaacg gcgtaaaatt cacatcgttg atatttgctt
    17461 ttgttaactg ctcaacaaga tactgacgat tacgtaattc accgttcagt tccgcaacgt
    17521 cagtgaccgc tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag
    17581 agaaatcggg gtcatcttta acggcctgac cattactgta tacgggcttt ttaattcccg
    17641 ccgcgttggc aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt
    17701 tcgttttaat gcgatcctga gccagttttt ctttttcaaa ctgggttttg atctccagtt
    17761 tattacggac gttttctatc gactctttca ccaccaacgc agagatataa tcgatatacc
    17821 cgctcaaaac ggtctgcgcc tctttactgg ttggcgcggt aaaacttagc gtccaggagg
    17881 tatacagtga cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt
    17941 cgctcaatgc aacaatagcg cgatgcaaat ccagttcatc gattttcgcc tcttttaatt
    18001 ggtccatcac ataaggtgat gaacgcaggt actcttccag caagctaaca gactgaaact
    18061 tcttgataaa caggttaaat gcaccgccac ggtcgatatt aacatcgaga tcaagaacgc
    18121 ggagtttggt aaaggttttc tccaggtcct gccactgaat ggcttcggcg ggagtaataa
    18181 cggctgagct ggtccatttt tgcggcagga tgaaagagat cagtaaacct gcgcaggcaa
    18241 atgcaaaaac gaccgccatg accgtttttt tggcccgcca taaaacttcg attagattaa
    18301 gtaaatcaat ttcattatta ctgggcgacg ccagaggata ctcagaaaaa tgagcgtcac
    18361 ttccctgttt aatattcaat gatgacatgg ggtataacct gaaaatgagt ccaatagggg
    18421 aaaatctcca cggcgctcac tttaccagtt ggtgagaatt atccgaagct gaagtttgta
    18481 aattccttcc actgaatgca acattcactc tatttaagag aa
//
LOCUS       DAZFVO020000097        18230 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFVO020000097 DAZFVO020000000
VERSION     DAZFVO020000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.098"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(190..1782)
                     /gene="lsrK"
                     /locus_tag="ACHKNB_RS15770"
                     /old_locus_tag="pgaptmp_003154"
     CDS             complement(190..1782)
                     /gene="lsrK"
                     /locus_tag="ACHKNB_RS15770"
                     /old_locus_tag="pgaptmp_003154"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HGU4196617.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1861..2814)
                     /gene="lsrR"
                     /locus_tag="ACHKNB_RS15775"
                     /old_locus_tag="pgaptmp_003155"
     CDS             complement(1861..2814)
                     /gene="lsrR"
                     /locus_tag="ACHKNB_RS15775"
                     /old_locus_tag="pgaptmp_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HGU4196618.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3063..4598
                     /gene="lsrA"
                     /locus_tag="ACHKNB_RS15780"
                     /old_locus_tag="pgaptmp_003156"
     CDS             3063..4598
                     /gene="lsrA"
                     /locus_tag="ACHKNB_RS15780"
                     /old_locus_tag="pgaptmp_003156"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HGU4196619.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVEAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4592..5620
                     /gene="lsrC"
                     /locus_tag="ACHKNB_RS15785"
                     /old_locus_tag="pgaptmp_003157"
     CDS             4592..5620
                     /gene="lsrC"
                     /locus_tag="ACHKNB_RS15785"
                     /old_locus_tag="pgaptmp_003157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HGU4196620.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            5620..6612
                     /gene="lsrD"
                     /locus_tag="ACHKNB_RS15790"
                     /old_locus_tag="pgaptmp_003158"
     CDS             5620..6612
                     /gene="lsrD"
                     /locus_tag="ACHKNB_RS15790"
                     /old_locus_tag="pgaptmp_003158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HGU4196621.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVLLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6624..7646
                     /gene="lsrB"
                     /locus_tag="ACHKNB_RS15795"
                     /old_locus_tag="pgaptmp_003159"
     CDS             6624..7646
                     /gene="lsrB"
                     /locus_tag="ACHKNB_RS15795"
                     /old_locus_tag="pgaptmp_003159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HGU4196622.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVTPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7673..8548
                     /gene="lsrF"
                     /locus_tag="ACHKNB_RS15800"
                     /old_locus_tag="pgaptmp_003160"
     CDS             7673..8548
                     /gene="lsrF"
                     /locus_tag="ACHKNB_RS15800"
                     /old_locus_tag="pgaptmp_003160"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HGU4196623.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEICWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8572..8862
                     /gene="lsrG"
                     /locus_tag="ACHKNB_RS15805"
                     /old_locus_tag="pgaptmp_003161"
     CDS             8572..8862
                     /gene="lsrG"
                     /locus_tag="ACHKNB_RS15805"
                     /old_locus_tag="pgaptmp_003161"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HGU4196624.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8919..9677
                     /gene="tam"
                     /locus_tag="ACHKNB_RS15810"
                     /old_locus_tag="pgaptmp_003162"
     CDS             8919..9677
                     /gene="tam"
                     /locus_tag="ACHKNB_RS15810"
                     /old_locus_tag="pgaptmp_003162"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HGU4196625.1"
                     /translation="MSDWNPSLYVHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9681..10595)
                     /locus_tag="ACHKNB_RS15815"
                     /old_locus_tag="pgaptmp_003163"
     CDS             complement(9681..10595)
                     /locus_tag="ACHKNB_RS15815"
                     /old_locus_tag="pgaptmp_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HGU4196626.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10802..12253)
                     /gene="uxaB"
                     /locus_tag="ACHKNB_RS15820"
                     /old_locus_tag="pgaptmp_003164"
     CDS             complement(10802..12253)
                     /gene="uxaB"
                     /locus_tag="ACHKNB_RS15820"
                     /old_locus_tag="pgaptmp_003164"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HGU4196627.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGVHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAQGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12480..13898)
                     /locus_tag="ACHKNB_RS15825"
                     /old_locus_tag="pgaptmp_003165"
     CDS             complement(12480..13898)
                     /locus_tag="ACHKNB_RS15825"
                     /old_locus_tag="pgaptmp_003165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HGU4196628.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14037..14396)
                     /locus_tag="ACHKNB_RS15830"
                     /old_locus_tag="pgaptmp_003166"
     CDS             complement(14037..14396)
                     /locus_tag="ACHKNB_RS15830"
                     /old_locus_tag="pgaptmp_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HGU4196629.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14396..15322)
                     /gene="glsB"
                     /locus_tag="ACHKNB_RS15835"
                     /old_locus_tag="pgaptmp_003167"
     CDS             complement(14396..15322)
                     /gene="glsB"
                     /locus_tag="ACHKNB_RS15835"
                     /old_locus_tag="pgaptmp_003167"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HGU4196630.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15386..16774)
                     /gene="sad"
                     /locus_tag="ACHKNB_RS15840"
                     /old_locus_tag="pgaptmp_003168"
     CDS             complement(15386..16774)
                     /gene="sad"
                     /locus_tag="ACHKNB_RS15840"
                     /old_locus_tag="pgaptmp_003168"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HGU4196631.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16875..17756
                     /locus_tag="ACHKNB_RS15845"
                     /old_locus_tag="pgaptmp_003169"
     CDS             16875..17756
                     /locus_tag="ACHKNB_RS15845"
                     /old_locus_tag="pgaptmp_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4196632.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17834..>18230
                     /gene="yneK"
                     /locus_tag="ACHKNB_RS15850"
                     /old_locus_tag="pgaptmp_003170"
     CDS             17834..>18230
                     /gene="yneK"
                     /locus_tag="ACHKNB_RS15850"
                     /old_locus_tag="pgaptmp_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4196633.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNP"
BASE COUNT         4599 a         4642 c         4525 g         4464 t
ORIGIN      
        1 cgctataaaa taagggttgt gcttaatgct ttaagaaaaa tagcaatttt cccttgaata
       61 tagtactggt gatggaacga tgaatctgca agctggcttt taacaagcca gctctaaaag
      121 aagggaaata agaataacta tactcaaaaa ctaacagcca cggtcatcat gatgtggctg
      181 tcaatgaaac tataacccag gcgctttcca taacgacgtc gtcagtccat gatcaaccag
      241 ccccagctga tcctgataaa ctgcctgcca cttatcgcgt gaatcctgat aaagttcatg
      301 cttttccggg tctggtgtgt gcgtccgttc ccagcgaacc aggcgttctc cggtttctgc
      361 cattgatgaa aaaattccgg caccgacgcc agctgcaatg gcacatccta atgcagtggc
      421 ttctttgacc accggaatat tgacgggtaa tcccgagaca tcagcgagaa tttgactcca
      481 taatttccct tttgaacctc cgcctgcaaa gactaacgat gaaggatgaa tattcgagaa
      541 atcagcaatt tgctgcaagt tacacgctga tacaatcgcc gcattttctt ccagcgcacg
      601 gaacaatgtc gctttgttac atttatccgg gtcaatggac aagttaataa aggaaggcgc
      661 agcgtgatac caggttttaa agcgcattct gtcggagaag atcggcatta cgccccacga
      721 cccaggcggc acccgactgg ccatctcttc cagcagcgta taggtgtcga tgcctaaacg
      781 ttcggcaatc agtttttctt cggcacagaa agcatcgcgg aaccagcgca tggtgagtcc
      841 ggtaaaaaag cttatagatt cagcttgtac catgccagga ataacatgag gattaacgcg
      901 cacgttcatt tctgggtctg tcaccggcgc ggctaaattt acaacttgct gccagaatgt
      961 gccgccaaga accgcggttt gtgccggacg cacaacgcct aacccaaggc aaccaagctg
     1021 cacgtcgcct cctccaacga ccaccggagt gcccgccttc agaccgcaga gttccgccgc
     1081 ttgtgaactt accacgccca gcaatgtgcc ggtttctttg acaggagaaa gaatatcggc
     1141 acgtaggcca gccatatcca gcaatgcagg tttccagtca cgggtggtta gatcaagaag
     1201 tcccgtggtg ccagcgttag agggatccac cgccagttcg ccgctgagca tataggccag
     1261 ccagtcgctg atcatggtga tggttgatgc ctgacggtaa atatcggaac gatggtgcgc
     1321 cagccaaagt aatctgggga tggcacttaa agccagtgtt tgtccggtcg cgcgataaac
     1381 ttcgttttca aaggtattgt tgtgcagttc tttaagttcg ctaacttcgc gtgccgctct
     1441 ggcatccaca ttggcgcagg cccagatcgg ggctccttca ttattatata aaacaatgcc
     1501 ttcacgcatc gaacatgccg aaacggcagc gatatactcc ggggctatgc cggcgttgtg
     1561 cagcgcctgg cgcatacact cacacgccag ttgccagttt ttgttgagat caaattccat
     1621 agaaccagga acgtccggta ctgccaggtg ccgccactcc gcctgtccca ctgctatttg
     1681 attgccttcc aggtcgaata tcacagcccg aatacttccg gtacctgcat ccagcgccat
     1741 caggtaatac tttgattctg aaggggtaaa gagtcgagcc attagattat cctcggctta
     1801 tgcgttctgt ctgttcctct atacgttctc catcattccc ggtaataagg tcatgcaaat
     1861 ttaactacgt aaaatcgccg ctgctgtgtc ctgatcggta accagtgcgt tgatataacc
     1921 gcctttcatt gcagcggcaa ttgcttcggc tttattttct ccccctgcca cgccaacccg
     1981 gacgggtatg gtcttcagcg agcttaaagg taagccaatc agttcgttat gtattttaat
     2041 atccgtgaca acgtcacctt ttgcatcaaa aaagtagcct aaaatgtcgc caaccgcccc
     2101 ttttcgacca atcattaact gttcgccctg gctgatataa ccggagcgaa tgatcgtcgc
     2161 gtcgtcctgt tgactgacgg ccccaatgcc gacaatcgcc acatcggctg cttgcgcggc
     2221 taacagaaca tctttgacgc aattttcatt ttttagcgta cgggcaatgt cagcggagga
     2281 tgcccgcaac ggagccggaa taatattcac actgcacgcc gcgttaagct gcccgatacc
     2341 cgtcatataa gaaccgacgc caccggagag cgtgaccagg cgaatttgct gtgacgaaat
     2401 aaaaccactt aagcgttgca gcgtattcat ggttgcctcg ccaaaaccaa tcgccagcat
     2461 ctgttgtggt tgaagtaaac tcatcaacat atgcgccgcg cctatcccca gtcgcccgcc
     2521 gacatcagca tccgcaagcc cagggatcac ccggacatgt tgcagcgaaa actgacgacg
     2581 taattgagtt tcatattcca gacagccttc aaagcgagaa ttaatctgta cgcgaataat
     2641 gccggactga tgccctttct ccagcaagcg cgacactttc aaacgggtca ggccgagacg
     2701 atcgctgatc tcgctctggg tcagcccgtc gtgatagtaa aaccacgcga tccgcgcgac
     2761 ctgttcttct tcacacattc cctgttctga aattgccgaa tcgttgattg tcataattca
     2821 ttcttcactt tgaacatatt taaatcttta atgcaattgt tcagttcttg cgcatttata
     2881 tctgtgatgg cagccacagt ttgactctac gagcatgaac aaacgcaacc gtgaaaatca
     2941 aaatagcata aattgtgatc tattcgtcgg aaatatgtgc aatgtccacc taaggttatg
     3001 aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg aacgggggaa
     3061 atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg gtttataaac
     3121 agtattcagg ggtcaatgtc ctgaaaggca tcgattttac gttgcatcag ggggaggtcc
     3181 acgccctgct cggcggcaat ggtgccggta aatcgacgtt aatgaagatt attgccggta
     3241 ttacccctgc tgatagcggt acgctggaga ttgggggcaa caactacgcc agattaacgc
     3301 cagttcatgc tcatcagctg ggcatttatc tcgttcccca ggaaccgctg cttttcccaa
     3361 gcctgtcgat aaaagaaaac atcctgtttg ggatggcaaa aaaacagctc tccatgcaaa
     3421 aaatgaagaa cttgctggcg gcgctgggct gccagtttga tctgcatagt ctggcaggat
     3481 cgctggatgt cgccgatcgc caaatggtgg aaatcctccg cgggctgatg cgcgactcgc
     3541 ggattctgat cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc gaacgcttgt
     3601 ttagtcgctt gcaagagctg cttgctactg gcgtgggtat tgtttttatc tcgcataagc
     3661 tgccggaaat tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga accatcgcct
     3721 taagcggcaa aaccagcgaa ctgtctaccg acgacattat tcaggccatc accccagcgg
     3781 tacgggaaaa atcgctctct gccagccaaa aattatggct ggagttacct ggtaaccgcc
     3841 cacaacatgc tgccggaacg ccggtgctga cactggaaaa tctgaccggc gaaggtttca
     3901 ggaatgtcag cctgacgctc aatgccggag aaattctggg cctggctggg ctggtggaag
     3961 ccggacgcac agaactggcc gagacgctct atggtctgcg tactttgcgt ggcggacgca
     4021 ttatgctgaa tggtaaagag atcaataaat tatccactgg agaacgttta ctgcgcggtc
     4081 tggtttatct gccggaagat cgccagtcat ccggactgaa tctcgatgct tcgctggcct
     4141 ggaacgtctg cgcccttact cataaccttc gtggattctg ggcgaaaacc gcgaaagata
     4201 atgccaccct ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa ccggaacaag
     4261 ctgcacggac attatccggt ggcaaccagc aaaaaatcct cattgccaaa tgcctggaag
     4321 cttcgccgca agtattgatt gtcgatgagc cgacgcgcgg cgtggatgtc tcggcccgta
     4381 atgatatcta ccagctgttg cgcagcatcg ccgcacaaaa tgtggctgtg ctgcttatct
     4441 cctccgacct ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg catcagggcg
     4501 aaattgccca ctctgcactg accgggcgcg atattaatgt cgagactatt atgcgcgttg
     4561 ccttcggcga tagtcagcgt caggaggcat catgctgaag tttattcaga acaaccgtga
     4621 aatcacggca ttgctggcgg tggtgctgct gtttgtatta cccggttttc tcgaccgcca
     4681 gtatttaagt gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc tgatcctgct
     4741 ggcaatgggc gcgacgctgg taatgcttac gcgcaatatt gatgtttcag tgggttcgat
     4801 taccggaatg tgcgcggtgc tgttggggat gttactgaac gcaggatatt cactacctgt
     4861 tgcctgtgtc gcgactttac tgcttggttt gctcgcggga tttttcaacg gtgtcctggt
     4921 cgcgtggcta aagatccctg ccattgttgc cacccttggc acgttagggt tgtacagagg
     4981 catcatgttg ctgtggactg gcggtaaatg gattgaaggg ttacccgccg aactgaaaca
     5041 gctctccgca ccgctgctgt ttggcgtttc cgcgattggt tggttgacga taattctggt
     5101 ggcatttatg gcctggctgc tggcaaagac ggcgtttgga cgcagttttt atgccacggg
     5161 cgataattta cagggcgctc gtcaactggg tgttcgtacg gaagccattc gcattgtggc
     5221 attttcgttg aacggctgca tggcggcact ggcgggaatt gtgtttgctt cgcagattgg
     5281 ttttatcccc aaccagaccg gtaccgggct ggagatgaaa gcaattgcag cctgcgtgct
     5341 gggcggcatt agtttgctcg gcggttccgg tgcgatcatc ggtgcggtac tcggcgcatg
     5401 gttcctgacg cagatcgata gcgtactggt gctgttgcgc attccggcat ggtggaacga
     5461 ttttatcgcg ggcctcgtgc tgctggcggt gctggtgttt gatggacgcc tgcgttgtgc
     5521 gctggaacgt aatctacggc ggcaaaaata tgcccgcttt atgacgccac cgccatccgt
     5581 taaacccgct tcgtcaggta aaaatcggga ggccgcataa tgcgtattcg ctacggttgg
     5641 gaactggctc ttgccgcact gctcgttatt gagattgtcg catttggtgc aattaacccg
     5701 cggatgttag atctcaatat gttgctgttc agcaccagtg actttatctg cattggcatt
     5761 gtcgccctac cgctgacgat ggtgattgtc agtggcggga tcgatatttc gtttggttcg
     5821 accatcggcc tctgcgccat tgcattgggc gtactgtttc aaagtggtgt gccgatgccg
     5881 ctggcgatac tcctgacctt actgctcggc gcattgtgcg ggctgatcaa cgccggatta
     5941 attatctata ccaaagttaa cccgctggtg attacgcttg gcacgctgta tctgtttgcc
     6001 ggaagcgctc tgctgctttc cggtatggcc ggagcgacgg ggtacgaagg tattggtgga
     6061 ttcccgatgg cgtttacaga tttcgctaac ctggatgtgc tgggactccc cgttccgctg
     6121 attatcttcc tgatatgtct cctcgttttc tggctctggc tgcataaaac ccatgccgga
     6181 cgtaatgtgt ttttgattgg gcaaagcccg cgcgtggcgc tttatagcgc gattccagtt
     6241 aaccgcacct tatgtgcgct ctatgccatg acggggctgg cgtctgcggt cgccgctgtg
     6301 ctgctggtgt cgtattttgg ttcagcacgt tccgatctcg gtgcgtcgtt tctgatgccc
     6361 gccatcaccg ccgtgctgct tggcggtgcc aatatttatg gtggttccgg ttccattatc
     6421 ggcaccgcca ttgcggtttt attagtggga tatttgcaac aaggtttgca aatggcagga
     6481 gtgccaaatc aggtgtccag cgccctttcc ggtgcgctac ttatcgtcgt tgtcgtaggt
     6541 cgttccgtta gcctgcatcg ccagcaaatt aaagagtggc tggcgcgtcg ggccaataac
     6601 ccattgccat aaaggatatc ttcatgacac ttcatcgctt taagaaaatc gccttactta
     6661 gcgctcttgg cattgccgca atctctatga atgtgcaggc cgcagagcgt attgcattta
     6721 ttcccaaact ggttggcgtg ggatttttta ccagcggtgg caacggcgca caacaagcgg
     6781 gtaaagagct gagcgttgat gtgacctacg acgggccgac agaacccagt gtttctggtc
     6841 aggtacagtt gattaataac ttcgtcaatc aaggttataa cgccattatc gtttctgcgg
     6901 ttacgcctga tggcttgtgt ccggcactga aacgcgccat gcaacgtggt gtgagagtgc
     6961 tgacctggga ctctgatact aaaccggagt gccgctctta ctacattaat cagggaacgc
     7021 ccgcccagtt gggaggtatg ttggtggata tggcggcgcg tcaggtgaat aaagacaaag
     7081 ccaaagtcgc gtttttctac tcaagcccca ccgttacgga ccaaaaccag tgggtgaaag
     7141 aagcgaaagc gaaaatcgcc aaagagcatc ctggctggga aattgtcact acgcagtttg
     7201 gctataacga tgccactaaa tcattacaaa ccgcagaagg aatattaaaa gcgtatagcg
     7261 atctcgacgc cattatcgcc cccgacgcca acgccctgcc cgctgccgca caagccgcag
     7321 aaaacctgaa aaatgacaaa gttgcgattg tcggattcag tacgccaaac gtgatgcgtc
     7381 catatgtgga acgcggcacg gtgaaagaat ttggcctgtg ggatgtggtt cagcaaggca
     7441 aaatttcagt gtatgtcgcg gatgcattat tgaaaaaagg atcaatgaaa acgggcgaca
     7501 agctggatat ccagggcgta ggtcaggtgg aagtctcgcc aaacagcgtt cagggctatg
     7561 actacgaagc ggatggtaat ggcatcgtac tgttaccgga gcgcgtgata ttcaacaaag
     7621 agaatatcgg caaatacgat ttctgatgtg cattacttaa ccggagtaag ttatggcaga
     7681 tttagacgat attaaagatg gtaaagattt tcgtaccgat caaccgcaac aaaatatccc
     7741 ttttatcctg aaaggttgcg gtgcgctgga ttggggaatg cagtcacgct tatcgcggat
     7801 atttaatccg aaaacgggta aaaccgtgat gctggccttt gaccatggtt attttcaggg
     7861 gccgactacc ggacttgaac gcattgatat aaatatcgcc ccgctgtttg aacatgccga
     7921 tgtattaatg tgtacgcgcg gcattttgcg cagcgtagtt cccccagcga ccaataagcc
     7981 ggtggtactg cgggcgtcag gtgcgaactc tattctggcg gaattaagta atgaagccgt
     8041 ggcgttatcg atggatgatg ccgtgcgcct gaacagttgc gcggtggcgg cgcaggttta
     8101 tatcggcagc gaatatgaac atcagtcgat caaaaatatt attcagctgg tcgatgccgg
     8161 aatgaaagtg ggaatgccga ccatggccgt gactggcgtg ggcaaagata tggtgcgcga
     8221 tcagcgttat ttctcgctcg cgactcgaat cgccgctgaa atgggggcgc aaattatcaa
     8281 aacctattat gtcgaaaaag gttttgaacg gattgttgcc ggatgtccgg tacccattgt
     8341 tattgctggc ggtaaaaaat taccggagcg cgaggcgctg gaaatttgct ggcaggctat
     8401 cgatcagggc gcttctggtg tggatatggg gcgtaatatt ttccagtctg accatccggt
     8461 ggcgatgatg aaagccgtac aggcggtggt tcaccataac gaaacggctg atcgggcata
     8521 tgaactctat ctgagtgaaa aacagtaact gcggatttaa ggagaagaat tatgcacgtc
     8581 acactggttg aaattaacgt tcatgaagac aagattgacg agtttatcga agtttttcgc
     8641 cagaaccacc tgggctctgt acaggaagaa ggcaatttgc gcttcgatgt cttacaggac
     8701 ccggaagtga attcgcgctt ttatatctac gaagcctata aagatgaaga cgcagtggca
     8761 ttccataaaa ccacgcccca ctacaaaacc tgtgtcgcga aactggaatc tttaatgacc
     8821 gggccgcgta aaaaacgtct gttcaatggg ttgatgccgt gaggcgaatt tatcattttt
     8881 atctacaatt ggggtaacgc gctgacggga gtaaataaat gtctgactgg aacccctctt
     8941 tatatgtgca ctttgccgct gaacgatcgc gtccggcggt ggagctgctt gccagagtac
     9001 cgctggagaa tatcgaatac gttgccgatc ttggctgtgg cccaggtaac agcaccgccc
     9061 tgctgcatca acgttggcct gcggccagga taacaggcat cgactcgtct ccggcgatga
     9121 ttgctgaagc gcgcagtgct ttgccagact gccagttcgt ggaagcagac atccgcaact
     9181 ggcaaccgga acaggcactc gatctgattt ttgctaatgc ctcactgcaa tggctgcccg
     9241 accactacga attgtttcct catctggttt ctttacttaa tccgcagggc gtgctggcag
     9301 tacagatgcc agataactgg ctggagccga cccatgtgct catgcgcgaa gttgcctggg
     9361 aacaaaacta cccggatcgc gggcgtgagc cgttggctgg cgttcatgct tactacgata
     9421 ttttgagcga agccggatgt gaggtcgata tctggcgaac cacctactat caccagatgc
     9481 cgtcacacca ggcgattatc gattgggtga ctgccactgg attacgtccg tggttacagg
     9541 atctgaccga gagcgaacag cagctttttc ttaagcgcta ccatcagatg ctggaagagc
     9601 agtatccact gcaagagaac ggacagatac tgctggcatt tccgcgtctt tttattgttg
     9661 cccggcgtat ggagtaaata tcacgtcagc tggtaatgac gatcgggaag aatttttgct
     9721 ggaatttcgg cttcatcgtt catctgtaac aggtcaattt caatagcgtt gcagatggca
     9781 tccagcggta aatcattgtt ttcagtaccg aacggatctt ccagttcttc cgccagacaa
     9841 tccagcgaaa taaaagtgta ggaaatcagc acagagataa aaggcgtcat gtaatgcagg
     9901 tccacgacca gcgcgaacgg cagcatgata caaaacagat aaacggtacg atgcaaaatc
     9961 agggtgtagg caaagggaat tggcgtatag gcaatgcgct cgcatcctgc caggaccgct
    10021 gaaatatcat taagccgatc gttgaggcta ataaacagga tatctgaaag ctgcccattg
    10081 cggcgctgaa ccgccaacca ctcccccatt attaacaaga tacggttagc tggagagttc
    10141 gaagccagta caggctgaag atcttcagtc ttgagataat gagccagcac ttccgcctgt
    10201 ggctgtttgc gtaatgtcat gcgtaaacag tgggcgaagg cgatttgcaa ccgggcaaac
    10261 tcccttacac ttgccgaatc cggcaatgtc gtttttacct cgcgcagtaa cgaccgtgag
    10321 gcaatcatca actgccccca aagttttcgc gcttcaacgt aacgggcgta cccggcatta
    10381 ttacgaaaac caagaaaaat ggcgatggcg acaccgagaa tgctgaacgg tgcgagggtg
    10441 aatttgatgc ccagatgcgt gtaccagggc agcatgaaaa taacagcgat agaaaagaga
    10501 aaattgagta gtaagcgcga tgatatcttg gataatactg agccgtgcca gacaaaaata
    10561 cggcgcagcc agtgttgttg tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc
    10621 gccctattaa acgtgattac gatcacattc tcaagacgct acttacaaat tacctacaag
    10681 cttacaacat taacaggcgc tgcatgtggc atctgttaat gataaaggca cataggatgt
    10741 tgtaactaat atagtgaaat aagaaacccg gtcgaaaccg ggttcagaag taacggtgtt
    10801 attagcacaa cggacgtaca gcttcgcgca tccctttttc aagaatcgca tccaggtcat
    10861 tagcaacctg ctcgaccaga ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt
    10921 ctgccaacac aatcgctacc agttcctggg taccgataac acggtcacga tgctggctcc
    10981 agagttgctg ataacgttcc agccagtgtg catcgtcctg taccggatat gtttctccgt
    11041 tacgctcacc acgatagaag gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa
    11101 gtgtgccttg tgccttctgc cctgccagca gctgcggcag gatgcgagtg cggaacttgg
    11161 tcataccgtt gagcgcgata gacagcagct gatgcttaat gtacgggtta cggaaacgcc
    11221 cggtgactgc actggcgaaa gattccagtt catcacgagg caaatccagt accggaataa
    11281 tttcttcgta gatagctttt tcaacgaatg cgcaaatttc agcatcgttc atcgcttcac
    11341 ctacggtatc cagccctgcc tggaacgcca ccggcaccag cgcggtgtgc acaccgttga
    11401 ggattgccac tttgcgttct ttgtacggtt taatatcgtc aacaatcagc acgttgagcg
    11461 gatatttgtc cagacgcagt tcagtcgcta aggatttcgg cccctgaatc acaaacaggt
    11521 aaaagtgttc agcggtgtcg aggaaaccat cgtgataacc cagttcttct tccagtttag
    11581 ccacttcatc gcgcggataa ccggtaacga tacggtccac cagcgtagaa cagaagctgt
    11641 tagcctgatc caaccattga ataaatgctt ctggcagtgc ccactcttga gcatagcgca
    11701 gcaccagttc acgcaacgcg tcgccattgt agtcgatcaa ctcacacggg atgatgatcc
    11761 aacctttatc cagcgcaccg ttgaaatggc taaaacgttc gaacagcaga cgggtcagtt
    11821 ttgcgggata gcttaccgct ggcgcgtcat cgaatttatc gcccgcatgg tagctgatac
    11881 ccgcttcggt ggtgttagag aaaacaaagc gcatttccgg attgtgcgcc agtttcagga
    11941 attcatcgta ttcactgtag acgctgattt cacgattaac agagcgaatc agacgcgcgt
    12001 cgctgaccgc ttcccccttc tcattcaggc cacggataat ggtggtgtac agaccatcct
    12061 gcgtgctcag tgacggcggg aatgaagttt caatcggacg aacaacgacc acgccagaat
    12121 tcagatcggt gtgctcattc aggagatcga tttgccagtc aacaaaggcg cgcaggaagt
    12181 taccttcacc aaactgaatg atacgttctg gatactgtgc accgggaaaa tcgcgacgat
    12241 ttagtgtttt cacaatgggt tcccttctga ttagtcatac aacctgtttg aattggtacg
    12301 acaggttagc aaactttaat acgccgaacc cctgttttga tcaactcctg atgattaatg
    12361 agcagtttta tgagaaaagt gtggcgcgga tcatggttta atcgaggaaa tacgcctttt
    12421 cctggatcat aaagtggtag aacacattgc attcaaatcg cgcggaatga ataaagatgt
    12481 cagacaactt cctcaccgta acgcatagtg ctggtacggt tgcgcccatc tttcttcgag
    12541 cgatacagac acgtatcggc ttcgaccatc aatttattaa aatcatcggt cagcgttcgg
    12601 tacgatgcgc gaccactacc gacgccaata cttaccgtga gataaagcgt tttttgttgc
    12661 caggtgaatg gttgcagttc aacgccttta cggatttttt ccgccattag cagaccatcg
    12721 acaggattca ccgacggcac tgcaacagca aattcttcgc cgcccatgcg cgccaccagc
    12781 cccttatcac cgacaatctt ctgaatatgc cgggcaaaca cgcttaacac tttatcgcca
    12841 cattcatgtc catagttatc gttaatgctt ttgaaatagt cgatatcaag cagcatgaca
    12901 gtcagatgtt gtgtctgttt cagcgatgga cttttcagcg cctcataaag accggaccgt
    12961 gagtaaacct gagtcagaaa atcaaagtcg gctcgcagcg caacttgctt cattagcgaa
    13021 ttgatcgctg ccacgctaaa agaaaccata attgggcata tcgccatcgt ggcaataccg
    13081 agacgtgcgg agaacatttg cggaatggag aacggcgaac cgaccgagat atcaatcacc
    13141 gaatttgcca ccagtacgat ttccaccgca ccggtgacaa aggtcaacag acatgttacc
    13201 tgcggcgtat agcgcactgc acaccagatt aatgcaggca gcgggaacgc cagactcccc
    13261 gctccgccaa tgactaccga ggcaataacc gacacaataa gcgcgatggc gggcatcatc
    13321 tgttctgctt taaagcgcgg cagcactcca ggaatagcca acgtcagcat acaaggcacg
    13381 atcaaaacgc ccgttgagaa ttgctcactg aaccagtccg caagcaaagg ccagaaatcc
    13441 agactatcaa tactgaccga accaatcgcc ccgacaatag cgcataataa tgcggcaatc
    13501 agacagtaat taaatagccg taaggcactt actggctcat acttattttt ccccaaacgc
    13561 ttatcccgca cgaccagtaa ggcgacggta acaataaaca tcatattgga gaaattgata
    13621 gccagtgaaa ccagtcccca ttcggtggtt atggcatcat aaaccagcat cgccacataa
    13681 ctgatcgcat agtaatgcag tcgattaagc cagacatagc gggcaaacac ccccgccatt
    13741 acgccattca gcggccagaa aagagaaagt tcgtgaacca gccgtaactc tgcaccaata
    13801 aaatagaaca gcgtggttag cgcaaaaata gcgatgctat tacgtagcag atgaccttcc
    13861 tggaacaaac ggaaagttga aatggggtgt acatgcatta attatctgtc catacaattg
    13921 cgggtggcaa ttatctcaac gtaataagta aattctcata aaggatagca tgttcgctga
    13981 cagcggcggc aaataaaggt accctgggaa tgctatttta tcgttttccg caattctcat
    14041 ggctgattca tctgtacaac cagccagtga tagattaccg ccacgatata acgttgttgt
    14101 tcttcgctta acgaaacacc ctgcggaatg ttatgccact tagccagaca accgcgacaa
    14161 caggttgcag tggcgtgctg agcgataaac actggatgcc cgcgcatggg ggtttgctta
    14221 ccgtcattag ccggtaatgc cggagcaaga cgtttagcga caaaatccgc cgcatgttgt
    14281 tcgatgactg gcgcgccttt ctccagacaa tactgacgct ctttcatgcc caaacgaaag
    14341 cgagatctaa attttgaacg ggataaacgc gcgaagagtg gatcgagaga ctgcattaat
    14401 aaaccgaacg ccctaactgt ttcgttaatt gttcaagaac ggcaatgccc gcaagcgagt
    14461 tacctgcatc atccagttcc ggactccaga ctgcgatggc catttcatgc ggaacaatcg
    14521 ccacaatacc gccaccaacg ccagatttcg ccggtagtcc cacccgccag gcaaactccc
    14581 ccgcgttctg gtacatacca ctggtcgcca tcagcgcgtt aatttgccgc gcctgcattg
    14641 gcgtcaccac aggttcatca atatgaatag ctttcccctg attagccaga aagacaaacg
    14701 tccgggccag ctctacacag ctcattttca gagcgcagta atgaaagtag ttttgcagaa
    14761 cggttgtcac gtcatgatgg aaattgccaa acgacttcat cagccaggcg atagccgcat
    14821 ttcgcgcgga atgttcaaat tcggaacgcg ctaccaccgt atcgtatgaa atatcagaca
    14881 caccgcttaa gccgcgcacg acttccagca tacgttgccg tggtgcgctt agtcgccctt
    14941 gcaacatatc gcagaccacc agcgcaccgg cattaatgaa cggattacgc ggaataccct
    15001 gttccatttc cagttgcact aaagaattga acggtgatcc agacggatct ttgccgacgc
    15061 gttgccagat ttcctcttcg gagtaatgac gcatggcgac aacgagactc agcactttgg
    15121 aaatagactg aatggaaaaa cgttcttgcg cgtctccggc ctgaaaaagc tgtccgtcaa
    15181 cggtacagat agcaatgccc aatcgggaac cgtctactgt agccagcgcc ggaatataat
    15241 ccgcgacttt accctgacca atgagcggcc gcacttgccg caagatgttt tctaaaattg
    15301 cattatccat ggcgactgcc actttctact cctggaccgc aggtctgaaa agacctgcga
    15361 gtatatcaga gctgaatatg tcgcgtcaga tccggtcttt ccacaccgtc tggatattac
    15421 agaattcgtg taagccgaaa tgggaaagct cacgaccaaa gccactcttt ttcacgccac
    15481 caaaggccac tcgcgcgtcg ctggcacaat aaccattgat aaacacccca ccgcattcca
    15541 gacgtgccgc catctgtctg gcctgtgttt cgtcggtggt aaaaatggtc gctgaaaggc
    15601 cgaactcact atcattagcc agttccagcg catgttctgc atctttcgca acggtgattg
    15661 ccgcaacagg gccaaacatt tcttcccgaa acgcggtcat ttctggggta acattcgcca
    15721 gaaccgttgg cggatagtag ttacctgccc cagccatctt ttccccgccc agtaacaaac
    15781 gcgcaccctg cgccagggtt ttctccacct gatgatgcag ctcatcacgt aaatcaaaac
    15841 gagccattgg tccgagagca ttctcttcat cacggggatc gcccattttc aaggctgccg
    15901 cagctgccac aaaacgttcg gtaaatgcag aagcaattcc ctcttcgata ataaagcgtt
    15961 ttgccgctgc acaaacctgt ccggtattct gataacgtcc ggctaccgcc gctttaaccg
    16021 ccagttccag atcggcatcg ttaagcacaa taaatggatc cgaaccgccc agttccagta
    16081 cgcatttttt cagtgccgct ccagcctgtg cgccaatagc cgctcccgca cgaacacttc
    16141 cggtcaccgt gacagcagca atgcgcgaat ctttaatcat ttgactgaca ccgtcgttgt
    16201 cggcattcag ccagccatat acgccttgcg ggattcccgc atctttaaac acctgggcaa
    16261 tgagctgagc acagcccatc acattcggcg catgtttaag taagtagccg ttacctgcaa
    16321 gaatgatggg aaccgcgcca cgcatcacct gccataacgg aaagttccac ggcataatcg
    16381 ccagaatcgt ccccaacggt cgatactcaa taactgcctg ctgattttcc accagcgtag
    16441 gttccgcctt cagcattgcc ggaccatgtt ctgcatacca gtcacacaaa ttcgccgatt
    16501 tcgccacttc agcgcgcgcc tgattgattg gtttgcccat ttcacgggtg atcatttgcg
    16561 ccatttcttc gctacgggcg cgcagagcct taccgatacc acgcagtttt tcagcacgat
    16621 aatctatatt tgtctcgcgc cagtcgcgaa agcctgctgc cgccagctga agtgcgtttt
    16681 cgatatcgtt agcgccagcc caaggcagca cagaaagttg ttcacccgtg gcaggattta
    16741 ttgaaattgc atgagttgcc ggagtaatgg tcatcggggt atctccttta tgagtcatgg
    16801 tatgaagata cgcagattta ctcttgcttt aaaatgaata atattaagcc acttattcac
    16861 gaatcgagaa tgttatggat ctgacccaac tggagatgtt caacgcggtt gccgaagctg
    16921 gcagcattac ccaggctgca gcaaaagtgc atcgcgtgcc gtcgaatctc actacccgtc
    16981 tacgccagct ggaaacagaa ctgggggtag atctgtttat tcgcgagaat cagcgtttac
    17041 gtctctctcc tgccgggcat aactttttac gctatagcca acaaattctc gcgttagtgg
    17101 atgaagcgcg gagcgttgtc gctggcgatg agccgcaagg tttattttct cttggttcgc
    17161 tggaaagcac cgcagcagtg cgcattccgg ccacgctggc ggaattcaac catcgttatc
    17221 ccaaaattca gttttcactt tccaccggcc cttccggcac tatgctggaa ggagtgctgg
    17281 agggaaaact gaatgcggca tttattgatg ggcccattaa ccatactgcc attgacggga
    17341 taccggtata ccgcgaggag ctgatgatcg tcacgccaca aggtcatgcg ccagtaatcc
    17401 gcgccagtca ggttaacggc agtaacattt atgccttccg cgccaattgt tcgtatcgtc
    17461 gccacttcga gagctggttt catgctgacg gtgccgctcc gggaactatt cacgagatgg
    17521 agtcgtatca cgggatgttg gcctgtgtgg tcgcaggagc aggcattgcg cttattccac
    17581 gctctatgct ggaaagtatg ccggggcatc accaggttga agcgtggccg ttagctgagc
    17641 aatggcgttg gttaacaacc tggctggtct ggcgtcgtgg tgcgaaaacg cgtccgcttg
    17701 aggcatttat tcaactactg gatgtgcctg actcggcaaa acagggttac caatgagcta
    17761 tttttgatag ttcttgcagc tcaaatgccc tatatagtga tgttccgatg acttatgact
    17821 atatggggca aatatggtta cgccagtaag catcagcaat tatatatctc ttcctgatga
    17881 ttttcccgct cgtaatattg cacctcaggt aaaagaagtt ttaaaggatt ttattgatgc
    17941 actcagtacc ataatatgtg atgaagagtg gcgcacgtct ttaaacatca actcagccac
    18001 aaaaaagata tttaataacc ttgataactt atcatatatt cagaggactt ctttccgggg
    18061 taacgacacg ctatacaatg aaaaggttca gtttaaactc acttatcccg tcaagaatgg
    18121 aagacacaaa gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga
    18181 taattttcgc catgatggag aaattaatat tttttgcgct cccaacccaa
//
LOCUS       DAZFVO020000098        18049 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFVO020000098 DAZFVO020000000
VERSION     DAZFVO020000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.099"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..374)
                     /locus_tag="ACHKNB_RS15855"
                     /old_locus_tag="pgaptmp_003171"
     CDS             complement(<1..374)
                     /locus_tag="ACHKNB_RS15855"
                     /old_locus_tag="pgaptmp_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196634.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFD
                     KLDNDSQIDNDFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPIT"
     gene            758..1810
                     /gene="yhiM"
                     /locus_tag="ACHKNB_RS15860"
                     /old_locus_tag="pgaptmp_003172"
     CDS             758..1810
                     /gene="yhiM"
                     /locus_tag="ACHKNB_RS15860"
                     /old_locus_tag="pgaptmp_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HGU4196635.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(2126..3328)
                     /gene="yhiN"
                     /locus_tag="ACHKNB_RS15865"
                     /old_locus_tag="pgaptmp_003173"
     CDS             complement(2126..3328)
                     /gene="yhiN"
                     /locus_tag="ACHKNB_RS15865"
                     /old_locus_tag="pgaptmp_003173"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGU4196636.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DFIAAKSS"
     gene            3560..5059
                     /gene="pitA"
                     /locus_tag="ACHKNB_RS15870"
                     /old_locus_tag="pgaptmp_003174"
     CDS             3560..5059
                     /gene="pitA"
                     /locus_tag="ACHKNB_RS15870"
                     /old_locus_tag="pgaptmp_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HGU4196637.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(5130..5465)
                     /gene="uspB"
                     /locus_tag="ACHKNB_RS15875"
                     /old_locus_tag="pgaptmp_003175"
     CDS             complement(5130..5465)
                     /gene="uspB"
                     /locus_tag="ACHKNB_RS15875"
                     /old_locus_tag="pgaptmp_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HGU4196638.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5856..6290
                     /gene="uspA"
                     /locus_tag="ACHKNB_RS15880"
                     /old_locus_tag="pgaptmp_003176"
     CDS             5856..6290
                     /gene="uspA"
                     /locus_tag="ACHKNB_RS15880"
                     /old_locus_tag="pgaptmp_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HGU4196639.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6389..6442)
                     /locus_tag="ACHKNB_RS15885"
                     /old_locus_tag="pgaptmp_003177"
     CDS             complement(6389..6442)
                     /locus_tag="ACHKNB_RS15885"
                     /old_locus_tag="pgaptmp_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196640.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6608..8077
                     /gene="dtpB"
                     /locus_tag="ACHKNB_RS15890"
                     /old_locus_tag="pgaptmp_003178"
     CDS             6608..8077
                     /gene="dtpB"
                     /locus_tag="ACHKNB_RS15890"
                     /old_locus_tag="pgaptmp_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HGU4196641.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8126..8878)
                     /gene="rsmJ"
                     /locus_tag="ACHKNB_RS15895"
                     /old_locus_tag="pgaptmp_003179"
     CDS             complement(8126..8878)
                     /gene="rsmJ"
                     /locus_tag="ACHKNB_RS15895"
                     /old_locus_tag="pgaptmp_003179"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HGU4196642.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8886..10928)
                     /gene="prlC"
                     /locus_tag="ACHKNB_RS15900"
                     /old_locus_tag="pgaptmp_003180"
     CDS             complement(8886..10928)
                     /gene="prlC"
                     /locus_tag="ACHKNB_RS15900"
                     /old_locus_tag="pgaptmp_003180"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HGU4196643.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            11131..11973
                     /gene="rlmJ"
                     /locus_tag="ACHKNB_RS15905"
                     /old_locus_tag="pgaptmp_003181"
     CDS             11131..11973
                     /gene="rlmJ"
                     /locus_tag="ACHKNB_RS15905"
                     /old_locus_tag="pgaptmp_003181"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HGU4196644.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATDIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            12045..13397
                     /gene="gorA"
                     /locus_tag="ACHKNB_RS15910"
                     /old_locus_tag="pgaptmp_003182"
     CDS             12045..13397
                     /gene="gorA"
                     /locus_tag="ACHKNB_RS15910"
                     /old_locus_tag="pgaptmp_003182"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGU4196645.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13451..13534)
                     /gene="dinQ"
                     /locus_tag="ACHKNB_RS15915"
                     /old_locus_tag="pgaptmp_003183"
     CDS             complement(13451..13534)
                     /gene="dinQ"
                     /locus_tag="ACHKNB_RS15915"
                     /old_locus_tag="pgaptmp_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HGU4196646.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            14043..14396
                     /gene="arsR"
                     /locus_tag="ACHKNB_RS15920"
                     /old_locus_tag="pgaptmp_003184"
     CDS             14043..14396
                     /gene="arsR"
                     /locus_tag="ACHKNB_RS15920"
                     /old_locus_tag="pgaptmp_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HGU4196647.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14450..15739
                     /gene="arsB"
                     /locus_tag="ACHKNB_RS15925"
                     /old_locus_tag="pgaptmp_003185"
     CDS             14450..15739
                     /gene="arsB"
                     /locus_tag="ACHKNB_RS15925"
                     /old_locus_tag="pgaptmp_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HGU4196648.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15752..16177
                     /gene="arsC"
                     /locus_tag="ACHKNB_RS15930"
                     /old_locus_tag="pgaptmp_003186"
     CDS             15752..16177
                     /gene="arsC"
                     /locus_tag="ACHKNB_RS15930"
                     /old_locus_tag="pgaptmp_003186"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HGU4196649.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16300..16596)
                     /locus_tag="ACHKNB_RS15935"
                     /old_locus_tag="pgaptmp_003187"
     CDS             complement(16300..16596)
                     /locus_tag="ACHKNB_RS15935"
                     /old_locus_tag="pgaptmp_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4196650.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16704..17705
                     /locus_tag="ACHKNB_RS15940"
                     /old_locus_tag="pgaptmp_003188"
     CDS             16704..17705
                     /locus_tag="ACHKNB_RS15940"
                     /old_locus_tag="pgaptmp_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4196651.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPIVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         4234 a         4568 c         4483 g         4764 t
ORIGIN      
        1 gtaatgggag acgataattt taacacatgg ttattgtctg gtataattgt aaagataaac
       61 gtatcagtag tattgtcggc gaatctatta tattcttgaa tatctataga aggttcacta
      121 ttataaaacc atactttctc catctggtga ctacgatcat ttaccttcca gtgaccatgt
      181 tgcaggaaat agatgatatg caggccaaaa tcgttatcta tttgcgaatc attatccagt
      241 ttatcaaatt caactatctt tgcacgatgt gttttatcaa ccttgccatg gtgttgatga
      301 ctgttaactt tatctgaatg cttatcttga tggtttgaag agaagtgctt aaatatattg
      361 tgaaatactt tcataaataa cctgtaatat ttttacttgt tagtaacaaa ataaacaata
      421 catgtctgaa agacatattt acgatgatac aaagcaataa tacttatatg ttcgatgatt
      481 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
      541 ataattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
      601 ggccttttaa ccggtaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
      661 gcatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
      721 catttcatta ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtggct
      781 tttcaaatta atccctttga ttatgggatt aatttgcatc gcgttaggtg gctttgtgct
      841 ggaaagttca gggcaaagcg agtatttcgt cgcgggtcat gtgctgattt ctctggcggc
      901 catatgcctg gcattattca ctaccgcatt tattatcatt tcgcagctca cgcgcggcgt
      961 taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat
     1021 gatatggggt tgggcactgt tagcaggcaa tgatgtgatg gcagacgagt ttgtcgccgg
     1081 ccatgttatt ttcggcgttg gtatgattgc cgcctgtgta tcgacggtgg cagcgtcatc
     1141 cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgatg gaacgccggt
     1201 acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct
     1261 gctcggtttc atttggtcta ttacgctgtt acgtagtgct gacataactc cgcattatgt
     1321 cgcgggtcac gtattgcttg ggttaaccgc aatctgtgcc tgtctaattg gccttgttgc
     1381 cacaattgtc catcaaacac gtaatacgtt ttcaactaaa gaacactggc tgtggtgtta
     1441 ttgggttatt tttctcggct caatcacggt actgcagggg atatacgtct tagtcagttc
     1501 cgatgcaagc gcccgactgg ctcccggcat tattcttatt tgcctcggaa tgatctgtta
     1561 cagcatattc tcaaaagtct ggctactggc actggtatgg agacgtacct gttcgttagc
     1621 caacagaata ccgatgattc ccgtcttcac ctgcctgttt tgccttttcc tggcatcgtt
     1681 tcttgcggaa atggcgcaga ccgacatggg atattttatt ccttcgcggg ttttggtcgg
     1741 tttaggagcg gtatgcttta cgttgttctc aatcgtttca atattagaag caggttctgc
     1801 taaaaaataa ttgcaacgta ccggataaaa ccagcgttga ccatttgcgt aacgctggtt
     1861 tttcttaggc atcatgaaat aacgcagcat taatgcatag tggttaagga taaaaaaaca
     1921 acaaagttac tgtttttttc aacctgttca tattttataa agatctggcc actgggtgag
     1981 ttttcaatca cctttccttc caccttatat taagcctgga gggtttcagt tcacgggctc
     2041 attagaaaat aatcacaaga tagccactat caaaattagc tcatttttaa tgcggctcta
     2101 ataattttca tctttaggta acaggtcagg acgacttcgc tgcaataaaa tcctgcgcac
     2161 aagcccacgc actcgaccac gcccactgga agttatagcc ccccagccag ccggtaacgt
     2221 ccatcacttc gccgatgaag tacagcccag gcactttgcg cgcttccatc gtccgtgaag
     2281 agagttcgtt ggtgtccacg ccgccgagcg tcacttcggc agtgcgatag ccttcagtgc
     2341 cgttgggttg tacgcgccag tcggtcaatg tgctaatcag tgcctgttgg tcacgcacgt
     2401 tgagctgttt tagcgaaaca tccgggattt gcccgagttg ctgtaagcgt tcaaccaacc
     2461 gcttcggtag atgaaccgcc agtgtgtttt tcaggctctg attcggatgt gcgttacgct
     2521 gctcattcag gaaggtttcg agatccacat ccggtagcag attgatactg acaaattcac
     2581 ccggttgcca gtagctggaa atctgcaaca ccgccggtcc agacaagccg cgatgggtga
     2641 agagtaagtt ctcacggaaa acgatgccgt tttcagcggt aatcacggaa ggcaccgcca
     2701 cgcccgccag cacctgtaac tcttcgagca acggtttatg cagagtgaat ggcaccagac
     2761 ccgcgcgggt cggcagcacg ttgaggccaa attgttcggc aatcttataa ccaaacggcg
     2821 acgcgcccag ccccggcatt gacagcccac cggtcgcgat gaccagcttt tcgcaaccga
     2881 cagtcatgcc gttcagttca agcgtgaagc ctgtttcatc cttcgccaca ctcagcactt
     2941 cgctacgcaa tctgaaggtt acattgccct tctcgcactc atccaccagc atgtcgacaa
     3001 tctgctgcgc ggagtcatcg cagaagagtt gtcctaacgt tttctcgtgc caggcgatgc
     3061 cgtgtttatt gaccagatca atgaaatccc actgggtaaa acgcgcgagt gcagacttac
     3121 aaaaatgcgg attctggctc agataagcgc ctggttcgac ataaaggttg gtaaagttgc
     3181 agcgcccacc gccagacata aggattttgc gccctggttt tttaccatta tcgatcagca
     3241 gaacccggcg tcctgcctga cctgccagcg cagaacagaa catacccgcc gcaccagcgc
     3301 ctataataat ggcatcaaac ctttccacgt tgcgctcctc ttagaaaaaa cgggcgtgaa
     3361 ttgtaaagat tcctcagtgg tcgcaccagc atcaatatta ctaaaaggaa gtatttgcct
     3421 gaattatata agataattat tttttgagtg aaatccatac aggaggcaaa tcaaaaaaag
     3481 tctatatttc actttgcccg cgccgcgaaa gtcactgata atgcgccgcg ttcatgtcct
     3541 caaaatggcg taacgtccta tgctacattt gtttgctggc ctggatttgc ataccgggct
     3601 gttattattg cttgcactgg cttttgtgct gttctacgaa gccatcaatg gtttccatga
     3661 cacagccaac gccgtggcaa ccgttatcta tacccgcgcg atgcgttctc agctcgccgt
     3721 ggttatggcg gcggtgttca actttttggg tgttttgctg ggtggtctga gtgttgccta
     3781 tgccattgtg catatgctgc cgacggatct gctgcttaat atgggatcgt ctcatggcct
     3841 tgccatggtg ttctctatgt tgctggcggc gattatctgg aacctgggta cctggtactt
     3901 tggtttacct gcatccagct ctcatacgct gattggcgcg atcatcggga ttggtttaac
     3961 caatgcgttg atgaccggga cgtcagtggt ggatgcactc aatatcccga aagtattaag
     4021 tattttcggt tctctgatcg tttcccctat tgtcggcctg gtgtttgctg gcggtctgat
     4081 tttcttgctg cgtcgctact ggagcggcac caagaaacgc gcccgtatcc acctgacccc
     4141 agcggagcgt gaaaagaaag acggcaagaa aaagccgccg ttctggacgc gtatcgcgct
     4201 gatcctttcc gctatcggcg tggcgttttc gcacggcgcg aacgatggtc agaaaggcat
     4261 tggtctggtt atgttggtat tgattggcgt cgcgccagca ggcttcgtgg tgaacatgaa
     4321 tgccactggc tacgaaatca cccgtacccg tgatgccatc aacaacgtcg aagcttactt
     4381 tgagcagcat cctgcgctgc tcaaacaggc taccggtgct gatcagttag taccggctcc
     4441 ggaagctggc gcaacgcaac ctgcggagtt ccactgccat ccgtcgaata ccattaacgc
     4501 gctcaaccgc ctgaaaggta tgttgaccac cgatgtggaa agctacgaca agctgtcgct
     4561 tgatcaacgt agccagatgc gccgcattat gctgtgcgtt tctgacacta tcgacaaagt
     4621 ggtgaagatg cctggcgtga gtgctgacga tcagcgcctg ttgaagaaac tgaagtctga
     4681 catgcttagc accatcgagt atgcaccggt gtggatcatc atggcggtcg cgctggcgtt
     4741 aggtatcggt acgatgattg gctggcgccg tgtggcaacg actatcggtg agaaaatcgg
     4801 taagaaaggc atgacctacg ctcaggggat gtctgcccag atgacggcgg cagtgtctat
     4861 cggcctggcg agttataccg ggatgccggt ttccactact cacgtactct cctcttctgt
     4921 cgcggggacg atggtggtag atggtggcgg cttacagcgt aaaaccgtga ccagcattct
     4981 gatggcctgg gtgtttaccc ttccggctgc ggtactgctt tccggcgggc tgtactggct
     5041 ctccttgcag ttcctgtaat cgtacacacc aaaacgagcg ggtcagttta ctggcccgct
     5101 ttttttatgc gttattattg ctgaagtgct taatgccaaa tcatcaatgc aatcaggctg
     5161 accaccacca gaccacacaa tgcgctggtc agaataaact gccgacgcac ccgctcacag
     5221 cggcgaataa attcatcatc gtgatgatcg cgataacgtt gggcatagat ataccaaacg
     5281 agacgcacct gtttgttggg ttggccatgt gaggtaaaaa agccccctcc atcaacatat
     5341 tgatagagca atggatcgca gttacgcagt accactaaca acgcgcgtag tgatgagaaa
     5401 tagcgcgcca tgttaacaat gcaaacgaca cataaagccc aaaataatgc gacggtgctt
     5461 atcatacctc ctccccggcg acctgcccgc ggagttccac cccggggcta ccgctcccga
     5521 tacgctgcca atcagttaac accaggtcct ggagaaaccg cttttgtggt gaccaacatc
     5581 cgagcggctc tatagatagt gtaggagatc aggttgtttt ttttccagaa ggttaaccac
     5641 tatcaatata ttcatgtcga aaatttgttt atctaacggg taagcaaggc ggattgacgg
     5701 atcatccggg tcgctataag gtaaggatgg tcttaacact gaatctttac ggctgggtta
     5761 gccccgcgca cgtagttcgc aggacgcggg tgacgtaacg gcacaagaaa cgctagctgg
     5821 ccagtcatcg acaactttat ggaaggagta acactatggc ttataaacac attctcatcg
     5881 cagtcgatct ctccccggaa agcaaagttc tggtagagaa agcagtctct atggctcgcc
     5941 cctacaatgc gaaagtttct ctgatccacg tagatgtaaa ctactctgac ctatacaccg
     6001 ggcttattga tgtgaatctg ggtgatatgc agaaacgcat ctctgaagag acacatcatg
     6061 cgctgaccga gctttccact aatgcaggct acccaatcac tgaaaccctg agcggcagcg
     6121 gcgacctggg ccaggttctg gtcgatgcaa tcaagaaata cgatatggat ctggtggttt
     6181 gtggtcacca ccaggacttc tggagcaaac tgatgtcttc cgcacgtcag ctgatcaaca
     6241 ccgttcacgt tgatatgctg attgttccgc tgcgcgacga agaagaataa tcatccctcc
     6301 acgacgtgtt cctgaacgcc cgcatatgcg ggcgttttgc tttttggcgc gccttgttac
     6361 ctgatgcagc gtaaacccct tatctggcct acgatctgcg tacgcaatca aaatctcctg
     6421 ccaacacaac atttaacacc atcatatttt ccatcattag tgtgatcagc tggttatttt
     6481 ctgttgtaat agtgtattaa tctattcacc gcatcaatat taagaatctc tgacagatgt
     6541 aaactttttc gcgcgttatc ccttacgcgt tcatactttt caggatggta ttggaaggtt
     6601 aataaatatg aatacaacaa cacccatggg gatgctgcag caacctcgcc catttttcat
     6661 gatctttttt gtcgagttat gggagcgatt cggctactac ggcgtgcagg gcgtactggc
     6721 ggttttcttc gttaaacagc ttggattctc gcaagagcag gcttttgtca cttttggtgc
     6781 ttttgctgcg ctggtctatg gcctcatttc cattggcggc tatgtcggcg accacctgct
     6841 ggggaccaaa cgcaccattg ttctcggagc acttgtgctg gcgattggct acttcatgac
     6901 cggcatgtcg ctacttaagc ctgacctgat tttcatcgcc ctggggacta tcgctgtcgg
     6961 taacggcctg tttaaagcta acccagccag cttgctttcg aagtgctatc cgccgaaaga
     7021 tccgcggctt gatggcgcat tcaccctgtt ctatatgtcg atcaatatcg gctcgttgat
     7081 agcgttatcg ctggcccctg tgatcgctga tagattcggt tattcagtca cctacaacct
     7141 gtgcggggcg gggttaatta tcgcattact ggtttacatc gcctgtcgtg gaatggtgaa
     7201 agacattggt tctgaacccg acttcaagcc gatgagcttc agcaaactgt tgtacgtatt
     7261 acttggcagc gtggtgatga tcttcgtatg tgcatggctg atgcacaacg tagaagtcgc
     7321 caatctggtg ctgattgttc tctccatcgt cgtcaccatc atcttctttc gtcaggcatt
     7381 caagctggat aaaaccgggc gcaataaaat gtttgtcgcc tttgtcctga tgctcgaagc
     7441 ggtggtgttt tacattctct acgcccagat gccaacatcg ctgaacttct ttgccatcaa
     7501 caacgtgcat catgaaattc tcggtttttc catcaacccg gtcagcttcc aggcgcttaa
     7561 cccgttctgg gtggtactcg ccagcccaat actggcaggc atttacacgc atctgggtaa
     7621 caaaggcaaa gacctctcga tgccgatgaa atttactctc ggcatgttta tgtgctctct
     7681 gggctttttg acggctgcag ctgcgggaat gtggtttgcg gatgcccagg ggctgacatc
     7741 gccatggttt atcgtgctgg tgtacttatt ccagagctta ggtgaactgt ttattagcgc
     7801 ccttggcctg gcgatgattg ctgccctggt gccgcagcat ttgatgggct ttattctcgg
     7861 gatgtggttc ctgacgcagg ctgccgcgtt cttgctgggc ggctatgtgg caacatttac
     7921 cgcggtgccg gacaacatta ccgatccgct tgagacgttg cccgtctata ccaacgtgtt
     7981 tggtaagatt ggtctggtca cactgggcgt tgcagtagtg atgctgttga tggtgccgtg
     8041 gctgaaacgc atgattgcga cgccggaaag ccattaatta ttcttgcaga aagcagggta
     8101 gcgttatcgc taccctggtt tacttttaca ctggcgtacc tgcataaata tcaaacctat
     8161 gccctttagt gactaccgcg tttggcgtgg cgacattcgc cagcggtggc gcgtagtccg
     8221 ggcgctttac caccacgcgt ttggtcgcca gcaagcgtgc aggctccagt aatccatcgg
     8281 catcaagatc cggtcccacc agcgactgaa agacacgcat ctcttttttc actagcgcgc
     8341 ttttctgctt atgcgggaac atcgggtcga gataaaccac ctgcgggcgc ggggtaatat
     8401 cagtcagcgc cgtcaggctg gaggcgtgaa ttaactgcaa ccgctcctgc aaccagccgc
     8461 cgatttccgc atccgcataa ccacgcgcca ggccgtcgtc gagcaatgcg gcaacgactg
     8521 gattacgctc cagcatccgc acgcggcagc cgactgaagc cagcacaaag gcatcgcgcc
     8581 ccagccctgc ggtggcatcc accacatccg gcaaataatc gcctttaatg cccaccgctt
     8641 tcgccaccgc ctcaccgcga ccaccgccga atttgcgtcg gtgcgccatc gctccgccaa
     8701 caaaatcaac aaagatgccg ccaagttttg gctcatcacg cttgcgcaat tccagatgtt
     8761 ccggcgttaa caccagcgcc atcaggttgt cttcatcgtg ctccagcccc cagcggaccg
     8821 ccagaacaga taaggcaccg tctccggtgc ctgtttcatc aattaagcag attttcactg
     8881 aatgatcagc ccttaatgcc gtaatgctcc agcatcgcat ccagctgcgg ttcacgacca
     8941 cggaagcgtt tgaacagatc catcggctct tctgaaccgc cacggctcag aatgttgtcg
     9001 aggaacgact gcccggtttc acggttgaaa atgccctctt cctcaaagcg cgagaaagca
     9061 tctgccgcca gtacgtcagc ccacaggtag ctgtagtaac ctgcggcata accaccggcg
     9121 aaaatatggc tgaaagcgtg cgggaaacgg ccccaggatg gagacggtac tacggcaacc
     9181 agtttcttga tttctgccag agtttcgagg attttcgctc cctgatccgg gcggaactcg
     9241 gcatggaggc ggaaatcgaa caggccgaac tccagctggc gcagaataaa cagcgccgcc
     9301 tggtagttct tcgccgccag cattttatcc agcaactctt tcggcagcgg ttcgccggtt
     9361 tcgtagtgac cggagataaa cgccagcgcc tccggctccc agcaccagtt ttccataaac
     9421 tgactcggca gttcgaccgc atcccacggc acaccgctga taccggaaac accagcggtt
     9481 tcgatgcggg tcagcatatg gtgcaggccg tgaccgaact cgtggaacag ggtgatcact
     9541 tcgtcgtgag tgaacagcgc cggtttacca tttaccgggc ggttgaagtt acaagtcaaa
     9601 tacgcgaccg gtttttgcag cgaaccatca gctttacgca tctggcctac gcagtcatcc
     9661 atccacgccc cgccgcgctt gttttcacgg gcatacagat cgaggtagaa gctgccgcgc
     9721 agttcgttat tttcgtcata cagttcgaag aaacgtacat ccggatgcca gacatcaaca
     9781 tctttacgct ctttagcggt gatgccgtaa atacgtttca ccacttcaaa caggccgtta
     9841 accgctttgt tttctgggaa gtacggacgc agctgctcgt cgctgatgct gtagaggtgc
     9901 tgtttttgtt tttcgctgta gtacgcgata tcccacggct gcaactcatc gacgccaaat
     9961 tccgctttgg cgaaggcgcg cagttgtgcc agctcttttt cgccttgcgg acgcgcgcgt
    10021 tttgccagat cggttaagaa atccagcacc tgctgcgggt tttctgccat tttagtggca
    10081 agggatttaa aggcgtagtt ttcaaagccc agcagttgcg ccagttcgtg acgcagagcg
    10141 aggatctctt ccatcacctt gctgttatcc catttaccgg cgttcgggcc ttgatcggag
    10201 gcgcgggtgc tgtaagcgcg atacatctct tcacgcaagg cctggttgtc gcagtaggtc
    10261 attaccggca ggtagcttgg gatatccagc gtcagcaggt agccttccag ctctttcgct
    10321 tcggcctggg cttttgccgc agccagcgcg ctttcaggca tccccgccag ctccgcttcg
    10381 tcggtaacga gtttggtcca gcccatcgtc gcatcgagga cgttgttgct gtactggttg
    10441 cccagctcgg aaagacgagt ggcaatctca ccgtagcgtt gttgtttctc tattggcaga
    10501 ccgatgccag agagttcgaa gtcgcgcagt gcgttatcaa ccgctttttt ctgagccgtg
    10561 ttcagcgtgg cgtaatgatc gccatcgcgc aggtcgcgat acgctttata cagcccttca
    10621 tgttgcccta cccaggtgct gtattccgac agcagcggca gggtttgttc gtacgcttca
    10681 cgcagttccg ggctattttt caccgagttc aggtggctga ccggggagaa gatacgcccc
    10741 agcacatcgt ccacttccgc caacggctgg cagagatttt cccaggtgta cggtgctcct
    10801 tgcgctacta cgcgctccac attttcgcgg cagtcgttca gcgccttagt cacggctgga
    10861 acgacatgtt ccgggagaat tttagaaaac ggaggcaatt caaagggagt cagtaacgga
    10921 ttcgtcattc gcgcagtcct ggttaaagag gttaaggaag cgctcaacag gcgctttaca
    10981 taatgtgtgt agcatggggt taagtgtagt gaatttcaat gagaaacgtt acgctttcgc
    11041 ggcggcggcc tcttttcggt atactgtcct gatacgcttt tgtgcgcccc gaatacgggc
    11101 cgatttttac ttaccggaac acctttaccc atgctcagtt atcgccacag ctttcacgct
    11161 ggcaaccacg ccgacgtcct taaacatacc gttcagagcc tgatcatcga gtcgctgaaa
    11221 gagaaagata aaccgtttct ctatctcgac acccacgcag gggccgggcg ttatcagtta
    11281 ggcagcgaac atgccgagcg taccggcgaa tatctcgaag gcatcgcccg tatctggcag
    11341 caggacgatt tgcccgcaga actggaggcg tacatcaatg tggtaaaaca cttcaaccgt
    11401 agcggtcagt tgcgttacta ccccggttcg ccgttgattg ctcgccagct actgcgtgaa
    11461 caggacagcc tgcaactgac cgaactgcac ccgagcgatt acccgttgtt gcgttctgaa
    11521 tttcagaaag atagccgtgc gcgtgtcgaa aaagccgacg gtttccagca gcttaaggcc
    11581 aaactgccgc cggtttcccg ccgtggttta atccttatcg acccgccgta tgaaatgaaa
    11641 actgactatc aagcggtggt cagcgggata gcagaaggtt acaaacgttt cgccactggt
    11701 acttacgcac tgtggtatcc ggtggtgctg cgtcagcaaa ttaagcgcat gatccacgat
    11761 ctggaagcga ccgatattcg caaaattctg caaattgaac tggcggtact gccagacagc
    11821 gatcgccgtg gcatgaccgc ttccggcatg attgtgatta acccgccgtg gaaactggaa
    11881 cagcagatga ataacgtgct gccgtggctg cacagcaaac tggttccggc aggcaccggg
    11941 cacgccaccg taagctggat cgtgccggag taattgcagc cattgctggc acctattacg
    12001 tctcgcgcta caatcgcggt aatcaacgat aaggacactt tgtcatgact aaacactatg
    12061 attacatcgc catcggcggc ggcagcggcg gtatcgcctc catcaaccgc gcggctatgt
    12121 acggccagaa atgtgcgctg attgaagcca aagagctggg cggcacctgc gtaaatgttg
    12181 gctgtgtgcc gaaaaaagtg atgtggcacg cggcgcaaat ccgtgaagcg atccatatgt
    12241 acggcccgga ttatggtttt gataccacta tcaataaatt caactgggaa acgttgatcg
    12301 ccagccgtac cgcctatatc gaccgtattc atacttccta tgaaaacgtg ctcggtaaaa
    12361 ataacgttga tgtaatcaaa ggctttgccc gcttcgttga tgccaaaacg ctggaggtaa
    12421 acggcgaaac catcacggcc gatcatattc tgatcgccac aggcggtcgt ccgagccacc
    12481 cggatattcc gggcgtggaa tacggtattg attctgatgg cttcttcgcc cttcctgctt
    12541 tgccagagcg cgtggcggtt gttggcgcgg gttacatcgc cgttgagctg gcgggcgtga
    12601 ttaacggcct cggcgcgaaa acgcatctgt ttgtgcgtaa acatgcgccg ctgcgcagct
    12661 tcgacccgat gatttccgaa acgctggtcg aagtgatgaa cgccgaaggc ccgcagctgc
    12721 acaccaacgc catcccgaaa gcggtagtga aaaatgccga tggtagcctg acgctggagc
    12781 tggaagatgg tcgcagtgaa acggtggatt gcctgatttg ggcgattggt cgcgagcctg
    12841 ccaatgacaa catcaacctg gaagccgctg gcgttaaaac taacgaaaaa ggctatatcg
    12901 tcgtcgataa atatcaaaac accaatattg aaggtattta cgcggtgggc gataacacgg
    12961 gtgcagtgga gctgacaccg gtggcagttg cagcgggtcg ccgtctctct gaacgcctgt
    13021 ttaataacaa gccggatgag catctggatt acagcaacat tccgaccgtg gtcttcagcc
    13081 atccgccgat tggtactgtt ggtttaacgg aaccgcaggc gcgcgagcag tatggcgacg
    13141 atcaggtgaa agtgtataaa tcctctttca ccgcgatgta taccgccgtc accactcacc
    13201 gccagccgtg ccgcatgaag ctggtgtgcg ttggatcgga agagaagatt gtcggtattc
    13261 acggcattgg ctttggtatg gacgaaatgt tgcagggctt cgcggtggcg ctgaagatgg
    13321 gtgcaaccaa aaaagacttc gacaataccg tcgccattca cccaacggcg gcagaagagt
    13381 tcgtgacaat gcgttaaatg ttaaagggct aagagtagtg tgctcttagc ccttaattac
    13441 gtttccgcta tcagttcaga agctgaagca gaaagcggat cagttccagc agcgcaatta
    13501 acgccccaag aacgatgatt gctttatcaa tcacccgttt tctccatgcg atggagtgag
    13561 aatgcatccg cttactcatc cactgcctgt cacggcacat gccctattgt tagataatgg
    13621 cattctcact ccggccagag catcagttaa cggcaccacc cgtacttctg accaggactt
    13681 tgaaagcgtt tatgcgcatt accagagtga aaatgcctca gagctaactg gataatcata
    13741 cagtacattc aggttataaa accagcatgt ccttgcaata gtttcagtat ggtattagca
    13801 ttgatgcgtt agataatggc attctcactc cagccagtgc caccaactca gggctggaaa
    13861 gtaaaaaacc gacgcaaagt cggttttttt acgtcctgat tcagacctcc tttcaaatga
    13921 atagccaact caaaattcac acctattacc ttcctctgca cttacacatt cgttaagtca
    13981 tatatgtttt tgacttatcc gcttcgaaga gagacactac ctgcaacaat caggagcgca
    14041 atatgtcatt tctgttaccc atccaattgt tcaaaattct tgctgatgaa acccgtctgg
    14101 gcatcgtttt actgctcagc gaactgggag agttatgcgt ctgcgatctc tgcactgctc
    14161 tcgaccagtc gcagcccaag atctcccgcc acctggcatt gctgcgtgaa agcgggctat
    14221 tgctggaccg caagcaaggt aagtgggttc attaccgctt atcaccgcat attccagcat
    14281 gggcggcgaa aattattgat gaggcctggc gatgtgaaca ggaaaaggtt caggcgattg
    14341 tccgcaacct ggctcgacaa aactgttccg gggacagtaa gaacatttgc agttaaaaat
    14401 ttagctaaac acatatgaat tttcagatgt gttttatccg ggtggcatta tgttactggc
    14461 aggcgctatc tttgtcctga ccatcgtatt ggttatctgg cagccgaaag gtttaggcat
    14521 cggctggagt gcaacgctcg gcgcagtact ggcgttagtt acgggcgtgg tccatccggg
    14581 tgatattccg gtggtgtgga atatcgtctg gaacgcgacg gctgcgttta tcgccgtcat
    14641 tatcatcagc ctgctgctgg atgagtccgg cttttttgaa tgggcggcgc tgcacgtctc
    14701 acgctggggt aatggtcgtg gtcgcttgct gtttacctgg attgtcctgc tcggtgctgc
    14761 cgttgccgcc ctgtttgcca atgatggcgc ggcgcttatt ttgacaccga ttgtcatcgc
    14821 catgctgctg gctttagggt tcagtaaagg cactacgctg gcgttcgtga tggcggccgg
    14881 attcattgcc gataccgcca gcctgccgct tattgtctcc aacctggtga atatcgtttc
    14941 cgctgatttc tttggcctcg gctttcgcga atacgcctcg gtgatggtgc cggtggatat
    15001 cgccgcgatt gttgccacgc tggtgatgtt acatctctat tttcgcaaag atattccgca
    15061 gaactacgat atggcgctgc tgaaatctcc cgcagaagcg atcaaagatc ctgctacgtt
    15121 caaaactggc tgggttgttt tactgcttct gctggtggga tttttcgtcc tggaaccgct
    15181 cggcattccg gtgagcgcca ttgcagctgt gggcgcgctg atattatttg tcgtcgctaa
    15241 acgcggtcat gcgattaata cgggtaaagt cctgcgcggt gccccctggc agattgtcat
    15301 cttctcgctc ggcatgtatc tggtggttta tggcctgcgc aatgccggat taacggaata
    15361 tctttctggc gtactcaacg tgctggcgga taacggcctg tgggccgcga cgctcggcac
    15421 cggattcctc accgccttcc tctcttctat tatgaacaat atgccgacgg tactggttgg
    15481 cgcgttgtcc attgatggca gcacggcatc tggcgttatc aaagaagcga tggtttatgc
    15541 caatgtgatt ggctgcgatt tgggaccgaa aattacccca attggtagcc tggctacgct
    15601 actctggctg cacgtacttt cgcagaagaa tatgactatc agctggggat attacttccg
    15661 tacagggatt atcatgaccc tgccggtgct ttttgtgacg ctggctgcgc tggcgctacg
    15721 tctctctttc actttgtaat gagatattga tatgagcaac attaccattt atcacaaccc
    15781 ggcctgcggt acgtcgcgta atacgctgga gatgatccgc aacagcggta cagaaccgac
    15841 cattatctat tatctggaaa caccgccaac gcgcgatgag ctggtcaaac tcattgccga
    15901 tatggggatt tccgtacgcg cgctgttgcg taaaaacgtc gagccgtatg aggaactggg
    15961 ccttgcagaa gataaattta ctgacgatcg gttaatcgac tttatgcttc agcacccgat
    16021 tctgattaat cgcccgattg tggtgacgcc gctgggaact cgcctgtgcc gcccttcaga
    16081 agtggtgctg gaaattctgc cagatgcgca aaaaggcgca ttctccaagg aagatggcga
    16141 gaaagtggtt gatgaagcgg gtaagcggtt gaaataaagc ggcgatatcc actgcccata
    16201 gttggtaaag tgcgctttgt ttatgtcgga tgcgacgcta acgcgtctta tccgacctac
    16261 aaaaccgtgc atggcatgaa atctgtagcg cgtagcgcat caggcaattt tgctatttac
    16321 gcagcactcc tcgcgtaaga attcaattat cgcttccagc attgcgtatt gcgaaacaca
    16381 catcagcgtc cgactctcac ggttttgttt aaccagccct accgatacca gtgctgaaat
    16441 atggtgactg agtgtagagc cgggtatccc aagctgcttt tgcaactcac cgaccggcaa
    16501 accttgctcc cccgctttga ccaggtgttt gaatatgaac aaacgggtcg ggtgacctag
    16561 ctctttcagc gctttagcaa cttcttccaa ctgcatatga cacccccttt gtctggttga
    16621 tatttcgata ataccagaaa tatcttgatc tgaatttcat ttcgattatt ctggaaatat
    16681 agatttatcc aatgaggtta cgtatgagta gttggcttgc gatgctgcaa gatgccgcag
    16741 agatgtttgt gtttctcgcc gtcgagcttt cgttgctgtt tatagtgatt agtgccggtg
    16801 tcagcctgat aagacaaaag gtgccagacc ataaaatcca gcagatgatg ggggccagaa
    16861 aagggagagg ctatctcctg gctgctctgt tgggagccgt taccccgttc tgtagttgct
    16921 cgacaatacc catgttacgt ggattgttgt cagcgaaagc cgggtttggt ccgactctca
    16981 cttttttatt tgtttcacca ttacttaatc ccattatcgt cggactaatg tggatgacct
    17041 ttggttggaa agtcaccttg ttgtacgcga ttatcgccgc cggcgtctcc gttcttgcca
    17101 gtattatcct ggattctctg gggtttgaac gtcatatcat tgccagtaaa agctcatcag
    17161 caaattgttg tgctccagca aaaacttcgc cgggaacgac atatacgccc ataaaagtga
    17221 gttgttgtag tccagcggct aaagccattg agaaccccat agttacctgt tgcagtacca
    17281 aagctgtagt aagtattaat cccataaaac tagccaccaa agatgcgttg caacagttta
    17341 aagatgtact gccatatctt ttgttaggag tattaatagg atcttttatt tatggcttta
    17401 ttccctcagc gtggattgcc gctcatgcag gagcagataa tcccttcgcc atcccattaa
    17461 gcgccgttgt cggtattccg ctgtatatcc gggctgaggc agttatccct ctggcatctg
    17521 ttctgatgac aaaaggaatg ggtctgggag cattaatggc attaatcatc ggcagtgccg
    17581 gcgcaagcct gacggaagtg atattactta aatcaatgtt cagaatgccg atgatagctg
    17641 cattcctgac ggttatatta ggtatggcta tcttgatggg ctatttgact caattgctat
    17701 tttaaataga tgccagcagt aaactataag atgcatagtt agtgccatat ccttctaagt
    17761 agaaaatcat agacaggata atccttaaaa atcacaaatg atttaactaa tgcattgaga
    17821 atgatgacgg ctcggtcaac aagtcatcaa cagacaataa ccacgtaatc atgcttctct
    17881 gacattttat cggagaagca atgactcttt gtctcagtac cctcctcctt tttaaaaggc
    17941 agatttcagt cgctaataca acacattttt aaatacactt taacctggtt cattattaca
    18001 catctaaaac atcacaaaaa tattacttca cacactatcg gagaacata
//
LOCUS       DAZFVO020000099        18029 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFVO020000099 DAZFVO020000000
VERSION     DAZFVO020000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 18029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.100"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            99..560
                     /gene="yehR"
                     /locus_tag="ACHKNB_RS15945"
                     /old_locus_tag="pgaptmp_003189"
     CDS             99..560
                     /gene="yehR"
                     /locus_tag="ACHKNB_RS15945"
                     /old_locus_tag="pgaptmp_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HGU4196652.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(600..1070)
                     /gene="yehS"
                     /locus_tag="ACHKNB_RS15950"
                     /old_locus_tag="pgaptmp_003190"
     CDS             complement(600..1070)
                     /gene="yehS"
                     /locus_tag="ACHKNB_RS15950"
                     /old_locus_tag="pgaptmp_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HGU4196653.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(1117..1836)
                     /gene="btsR"
                     /locus_tag="ACHKNB_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /gene_synonym="yehT"
     CDS             complement(1117..1836)
                     /gene="btsR"
                     /locus_tag="ACHKNB_RS15955"
                     /old_locus_tag="pgaptmp_003191"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HGU4196654.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1833..3518)
                     /gene="btsS"
                     /locus_tag="ACHKNB_RS15960"
                     /old_locus_tag="pgaptmp_003192"
     CDS             complement(1833..3518)
                     /gene="btsS"
                     /locus_tag="ACHKNB_RS15960"
                     /old_locus_tag="pgaptmp_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HGU4196655.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3740..4471
                     /gene="mlrA"
                     /locus_tag="ACHKNB_RS15965"
                     /old_locus_tag="pgaptmp_003193"
     CDS             3740..4471
                     /gene="mlrA"
                     /locus_tag="ACHKNB_RS15965"
                     /old_locus_tag="pgaptmp_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HGU4196656.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4531..4638
                     /gene="yohO"
                     /locus_tag="ACHKNB_RS15970"
                     /old_locus_tag="pgaptmp_003194"
     CDS             4531..4638
                     /gene="yohO"
                     /locus_tag="ACHKNB_RS15970"
                     /old_locus_tag="pgaptmp_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HGU4196657.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4619..5350)
                     /gene="yehW"
                     /locus_tag="ACHKNB_RS15975"
                     /old_locus_tag="pgaptmp_003195"
     CDS             complement(4619..5350)
                     /gene="yehW"
                     /locus_tag="ACHKNB_RS15975"
                     /old_locus_tag="pgaptmp_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HGU4196658.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5355..6281)
                     /gene="yehX"
                     /locus_tag="ACHKNB_RS15980"
                     /old_locus_tag="pgaptmp_003196"
     CDS             complement(5355..6281)
                     /gene="yehX"
                     /locus_tag="ACHKNB_RS15980"
                     /old_locus_tag="pgaptmp_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HGU4196659.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6274..7431)
                     /gene="yehY"
                     /locus_tag="ACHKNB_RS15985"
                     /old_locus_tag="pgaptmp_003197"
     CDS             complement(6274..7431)
                     /gene="yehY"
                     /locus_tag="ACHKNB_RS15985"
                     /old_locus_tag="pgaptmp_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HGU4196660.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7438..8355)
                     /gene="osmF"
                     /locus_tag="ACHKNB_RS15990"
                     /old_locus_tag="pgaptmp_003198"
     CDS             complement(7438..8355)
                     /gene="osmF"
                     /locus_tag="ACHKNB_RS15990"
                     /old_locus_tag="pgaptmp_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HGU4196661.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8566..10863)
                     /gene="bglX"
                     /locus_tag="ACHKNB_RS15995"
                     /old_locus_tag="pgaptmp_003199"
     CDS             complement(8566..10863)
                     /gene="bglX"
                     /locus_tag="ACHKNB_RS15995"
                     /old_locus_tag="pgaptmp_003199"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HGU4196662.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11059..12774
                     /gene="dld"
                     /locus_tag="ACHKNB_RS16000"
                     /old_locus_tag="pgaptmp_003200"
     CDS             11059..12774
                     /gene="dld"
                     /locus_tag="ACHKNB_RS16000"
                     /old_locus_tag="pgaptmp_003200"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HGU4196663.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12812..13744)
                     /gene="pbpG"
                     /locus_tag="ACHKNB_RS16005"
                     /old_locus_tag="pgaptmp_003201"
     CDS             complement(12812..13744)
                     /gene="pbpG"
                     /locus_tag="ACHKNB_RS16005"
                     /old_locus_tag="pgaptmp_003201"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HGU4196664.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13918..14505)
                     /gene="yohC"
                     /locus_tag="ACHKNB_RS16010"
                     /old_locus_tag="pgaptmp_003202"
     CDS             complement(13918..14505)
                     /gene="yohC"
                     /locus_tag="ACHKNB_RS16010"
                     /old_locus_tag="pgaptmp_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HGU4196665.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLDIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14675..15253
                     /gene="yohD"
                     /locus_tag="ACHKNB_RS16015"
                     /old_locus_tag="pgaptmp_003203"
     CDS             14675..15253
                     /gene="yohD"
                     /locus_tag="ACHKNB_RS16015"
                     /old_locus_tag="pgaptmp_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HGU4196666.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15383..16144)
                     /gene="yohF"
                     /locus_tag="ACHKNB_RS16020"
                     /old_locus_tag="pgaptmp_003204"
     CDS             complement(15383..16144)
                     /gene="yohF"
                     /locus_tag="ACHKNB_RS16020"
                     /old_locus_tag="pgaptmp_003204"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4196667.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16197..17633)
                     /gene="mdtQ"
                     /locus_tag="ACHKNB_RS16025"
                     /old_locus_tag="pgaptmp_003205"
     CDS             complement(16197..17633)
                     /gene="mdtQ"
                     /locus_tag="ACHKNB_RS16025"
                     /old_locus_tag="pgaptmp_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HGU4196668.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGCSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17857..17940
                     /gene="yohP"
                     /locus_tag="ACHKNB_RS16030"
                     /old_locus_tag="pgaptmp_003206"
     CDS             17857..17940
                     /gene="yohP"
                     /locus_tag="ACHKNB_RS16030"
                     /old_locus_tag="pgaptmp_003206"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HGU4196669.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4394 a         4926 c         4471 g         4238 t
ORIGIN      
        1 ctccagttcc ggactgctca cgtcacggta ttaggcatat tctatatagc ccctggtgag
       61 agtcaccagt ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt
      121 tttccctcgt tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag
      181 aatcgaagaa attcagcgcc aatctgaacg gcactgaaat tgccattacc tatgtctaca
      241 aaggtgacaa ggtgcttaag caatcttctg aaaccaaaat tcaatttgct tctattggtg
      301 caaccaccaa agaagacgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca
      361 tcgcgggtgt tgaagaaaaa ttaacctata cagataccta cgcgcaggaa aacgtgacta
      421 tcgatatgga aaaagtggat tttaaagccc tgcagggtat ttcaggaatc aacgtttctg
      481 ctgaagatgc caaaaaaggt atcactatgg cgcaaatgga actggtgatg aaagccgctg
      541 gttttaaaga agtgaaataa tcggttggcg gtcatgctct aaacatgacc gccaattttt
      601 tagccttttt tcacatgctg gcgcgctgcc agtccacgca gaaaataacg taaaaattga
      661 tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgctgt aatttccggc
      721 atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt tttcagcgaa
      781 aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc cggctccagt
      841 gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag gaatgacgac
      901 aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag ccagacggcg
      961 atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag gtcattatta
     1021 ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat gaggccttca
     1081 ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg cctcttttaa
     1141 gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa tcaactcggc
     1201 ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca gatactgacg
     1261 atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt cggtaaagcc
     1321 ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga caaatgccac
     1381 atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa atttcagcgc
     1441 ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgttcctgac gcaaacgcgc
     1501 cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa aggcatgttc
     1561 ttcaaaggct ttaatagcgt attcgtcaaa cgcggtgaga aaaacaatat acggacgatg
     1621 ttccgggtcg agcatcccca ccatttccag accactgatg cgcggcatct ggatatcgag
     1681 aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg cgtttgaaca
     1741 ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt tctcccgtgc
     1801 taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca tggtagtcgt
     1861 aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata gtcatcgcca
     1921 aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc attggttacc
     1981 ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc ctcacgtcgg
     2041 gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt aatggcgttt
     2101 tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc ttgcggaata
     2161 gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag ataagcgttc
     2221 acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa gtttttgcgg
     2281 aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg gcggatcacc
     2341 gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc gtgaagcagt
     2401 ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc aagaatctgc
     2461 gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact gaataaacgg
     2521 tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc acgcaacgga
     2581 attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata aggtacttcg
     2641 ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta agtcgaagaa
     2701 atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag caatttctct
     2761 cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg agccactttc
     2821 atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc tgccactttc
     2881 agcgcagtgg cagaaaaagc agaagtgtat ttttcaaaca tcgcgcgttt atcgagcaat
     2941 atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggtgc agcaatatta
     3001 ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg caaggatgat cagcatttgc
     3061 accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac tttatcagtg
     3121 cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc aacgatggtt
     3181 gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa gccgccggtc
     3241 agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac cgcgcctatc
     3301 gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc catgatgcag
     3361 aagatggaaa agacgatgta acagagaaat ttatgcggca gacgaaccgt gacctgcatt
     3421 aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa aacgcacatc
     3481 tgctgaagca gcagcaacac cagattaaaa tcgtacatac tcgcaaacca cacttccctt
     3541 taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc agaaaaatga
     3601 gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc ctatagatgc
     3661 acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa accgggacct
     3721 cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt tgctttgtga
     3781 tattaaccct gtcacgttac gcgcgtggca gaggcgttac gggttgctga aaccgcaacg
     3841 gacagacggc ggtcatcgac tgttcaacga tgccgatatt gaccgtatcc gcgagatcaa
     3901 acgctggatc gacaacggcg tgcaggtcag caaagttaaa atgctgctca gtaatgaaaa
     3961 tgttgatgtg cagaacggct ggcgcgatca gcaagaaaca ttactgacct acctgcaaag
     4021 cggcaatctg catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
     4081 gacactcacc acacatctgt ttattcctct gcgccgacga cttcaatgcc aacaaccgac
     4141 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
     4201 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
     4261 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
     4321 cctcgcccac tcgctcaatc aactacgccc tgaactattc gaaggccgta cattgctggt
     4381 gtggtgcggt gaaaatcgaa cctccgccca acagcagcaa ctcaccagtt ggcaagaaca
     4441 aggccatgat attttcccac tcggcattta atgattcgtt aacaaatgcg ctttactgta
     4501 caatcctttc gttaacataa ggagtgcatt atgcgcatag ctaaaattgg ggtcatcgcc
     4561 ctgttcctgt ttatggcgtt cggcggaatt ggtggcgtca tgctcgcagg ttataccttt
     4621 attttgcgtg ctggctaagc gcctgcacca gcctttcaaa caggcggtct gcgatgatcg
     4681 ccgccagtgc caccagtaac gccccctgga tcacatacgc ggtattaaat ccgctaagcc
     4741 cgatgatgat gggcgtaccc agcgtgctgg ctcctaccgt tgaggcgatc gtcgccgtac
     4801 caatgttgat aatcaccgaa gttcgcacgc ccgccagaat caccggagcc gccagcggta
     4861 gctcgacctt acgcagtcgc tgaccacgac tcattcccat acctttcgca acttctgtca
     4921 cgctggcatc gatcgctccc agcccggcaa gtgtcgcctg caggacgggc agcacaccgt
     4981 aaaggatcaa ggcgataatc gctggttgca gaccaaagcc gatcaccgga acggcaatcg
     5041 ccagcactgc gacaggcgga aaagtctgtc caacggcggc aatagtttcc accagtgggc
     5101 gaaattccgc gccccacggg cgagtgacag caattccggc accagtgcca atgatcaccg
     5161 caaacaaact cgaaattccc accagccaga aatgagccag tgccagagct gcaaaacttt
     5221 cttgctgata aacgggtcgt ggcagttgtg ggaacaaggc agcaaacagc ggctggctgt
     5281 aaggcagcca gaaaatcagt gccacaaaca aagcaatgag ccagaacagc ggatcgcgca
     5341 acatcttcat acgcttacgc ctccaccagc agatcctgaa aatgcagcgt accgcaaggc
     5401 tggccctgcg tgttcaccac cggcagcacc tcgcaacccc gcgcgacaaa cagggagagc
     5461 gcatcgcgta gcgtcatctc ttctaccagt gcctcacctt ctgcccgttc ttcgcgacgc
     5521 acgtaatccg ccacactacg taacgaaagc agacgcacac ccagttcgct acgtccaaaa
     5581 aactggcgga caaaatcatt cgccggacga gtcagcatcg tcagcggatt gccctgctgc
     5641 accacttcac cgtgatccat caataccaga tgttcagcca gccgtagcgc ctcatcaata
     5701 tcatgagtga ccagcacaat agtgcgcccc agcaaacggt gaatgcgcgt catctcttgt
     5761 tgcaacgcgc cgcgcgttac cgggtccagt gcgccaaaag gttcatccat cagtaagact
     5821 tgcggatcgg cagccagtgc gcgcgccact cccacacgtt gctgctgacc accggaaagc
     5881 tgatgcggat aacgctcacg caaatttgac tccagcccca gtagcgtcat taattcgtcg
     5941 atacgatcgt caatccgtgc ccgcgaccat ttttgtaatt gcggcacggt agcgatgttt
     6001 tgcgccacgc tccagtgggg gaacaggcca atagattgaa tggcatagcc catccggcgg
     6061 cgcaactcca gtactggcag cgagcgaatt tcttctccgg caaagcggat ctctccgctg
     6121 tcatgttcca ccaggcggtt aatcattttc aaggtggtgg atttgccgga gccagatgtg
     6181 ccaatcagca ccgaaaaact cccttcctga aaattgagat taagatcgtt aacggctttt
     6241 tgtgcgccaa acagtttgct gacatggcta aattcaatca ttacgtttca ccttcagcag
     6301 tgcgataagc aaatcgaaca gggcatcgat cagcaccgcc agaacaatta ccgggatcac
     6361 ccccagcaac actaaatcaa tggcgctgct tagcagcccc tggaaaacca gcgcaccaaa
     6421 accgcctgcg ccgattaacg ccgcaatcac cgccatacct acagtttgca ccatcaccac
     6481 ccgcaggctg cgcagaaata ccggtaacgc cagtggtaac tgaacatgca ggaatcgctg
     6541 cgccccgctc atgcccatcg ctctggcgct ctccagcaca tcgcgcggga tctggttcaa
     6601 accgactacc acgccgcgca ccagcggcag caaggcatag agcaccagcg caatcagtgc
     6661 gggtgtcatt ccggttcctg ctatgccgag cttccccagc cacggaaagg ccgtaaccag
     6721 cgcggcaagc ggcgcaatca acaggccaaa gagcgccacc gaaggcacgg tctgaatgac
     6781 attgagcaga gaaaaaattg ccccctgccg cgcagtggaa aagtagcacc agatgcccaa
     6841 cggcacacca atcactaacg caggcagcac cgcaccaaac agcaacgtca gatgttgtgc
     6901 cagcgcgtcg tcaaacacat cctgacggtt ggcgtattct ttcattagtg agagatcgtt
     6961 aagcgtgccg gagtacagca accacagcgg aataatggca atctgcatat gcaacaacca
     7021 gcgccacagc ggatgcgtgg agattcggcg gatggcatcg ctacaggcca gcaatgccag
     7081 cgccgcagcc agccagaaac cactgccgag gctggtacgc gccagcgcac tgccattttg
     7141 cgccagttgg gtcgccgcct ttccagctcc ccacaccaac aatacgaaga cgaattgcgc
     7201 cagaatgagt gcacaaatgc tgcctttttt agcggggaca aaacaggctg tcagccaggc
     7261 gcaaccaacg cccaccagca tccagatcgt ttgcggccac agttgccaga gatgacgccc
     7321 ttcacccgag actaaacgat taggcgcgta gctgataaac ggcagcgctg ctgcgattgc
     7381 cgtcagcaac agcagcagcg ccagaacagg attaatacgt agataagtca cgggaaatta
     7441 cttcgcccac cctttttgtt tcaggtagtc ggcagccact tttttggcat ccagtccttc
     7501 cactgcaatg ctggcattca gttgctgcaa tgtttttgca tcgaggctgg cgaagactgg
     7561 ctgtagccac tgtgccattt gcggatactc cttcaacacc gattcacgca ccactggtgc
     7621 aggcgcatag ataggttgca cgccttgcgg atcgcttaag gtttgcagcc ccagcgccgc
     7681 taccgggccg tcagtgccgt aagccattgc agcattaacg ccagaggttt gctgggcagc
     7741 ggctttgatc gtcaccgccg tgtcgccacc agccagtgac agcaactgat cctgaccgag
     7801 cttaaagcca taggcttttt caaacgcggg taacgcatcg gcgcgttcga taaactctgc
     7861 cgaggctgcc agtttgaagg tgccgccctc ttgcagataa cgactcaggt cggcaagcga
     7921 agtgagttta tttttctctg ccacatcctg acgcacggcg atagtccagg tgttatttgc
     7981 aggcgcgggc gtcagccaga ttaacttgtt gtgctccgaa tcgagctttt tgactttctc
     8041 gtaaccttgc tgcgcgtttt tccacgctgc atcgttttca tctttaaaga agaaagcgcc
     8101 attgccggta tattccggat agatatccag ttcaccggaa gtaatcgccc cgcgcaccac
     8161 aggagtcgtt ccgagttgca ctttattcac cgtcggtacg ccgtggcttt ccagcacctg
     8221 caaaatgata ttgccgagta gcgcaccttc ggtatcgatt tttgaaccga ctttaacggg
     8281 ggaagccgcc tgtagcggca ggctcacggc tgccaacata accagtgaac ctgcccagag
     8341 ctttaagagt ggcatgatgc tttcctcatt cttttactgt tgttttcagc gaattaagag
     8401 aaaagcatag ttgataatgg cggggttagc ttgagcggaa ggattcagtt gcagaatcag
     8461 ataaatactt aagaggcata ttcggtccgg cgtttcctgc cggatgcggc gcgagcgcct
     8521 tatccggcct acaaagggcg caaacgtcgc gcccttacta aagcattaca gcaactcaaa
     8581 ctcgcctttc ttaacgcgtg cggaatcagt gccgataaat acattgaact tgccaggctc
     8641 ggcgtcatat ttcatctgtt gattccagaa cttcagcgcc tcaatatcga tcgggaagct
     8701 gacagtctga gtttcgcccg gtttcagggt gattttctca aagcctttca gctgtttcac
     8761 agggcgactc atggaagccg tcacatcctg caagtacatc tgcactaccg tggccccctc
     8821 gcgcttaccg gtgttcgtca cctgcacgct ggcagtcact ttgccgtcac gcttcatggt
     8881 cggcgcagaa agtttcacat cagagacggt gaaagtggtg tagctcagcc catagccgaa
     8941 cggatacaac gccccgttag cttcatcaaa ataacgcgaa gtgtatttgt tcggcttgtc
     9001 ggcattatac gggcgaccgg tattcagatg gctgtagtac accgggatct gcccgacaga
     9061 acgcgggaag gacattggca gcttgccgga cgggttgtaa tcgccaaaca atacatcggc
     9121 aattgcatta ccgccttcag tccccgcaaa ccaggtttcc agaatcgcat cagcctgctg
     9181 atcttctttc accagcgcca gcggacgccc gttcatcagc accagcacca gcggtttacc
     9241 ggtggctttc agcgccgcaa tcaagtcacg ttggctttgc ggaatagtga tatcggtccg
     9301 gctggaggct tcgtgcgcca tcccctgtgc ttcaccgact acagccacca ccacatcaga
     9361 ttgtttcgcc gtctgcaccg cttcatcaat catctcttgc ggcgaacgcg gatcgacttt
     9421 gaccgcttct tcatactgat tcaggaaatc gataatgcct ttgtcactgg taacgttcgc
     9481 ccctttggca tacagcactt taccgttttc accgacggaa tttttaatcc cggtcagtac
     9541 ggtcacggat tgatcggcaa caccggctgc ggaccagctg cccatcacgt cacgtttact
     9601 gtccgccagt ggcccaacca ccgcaatggt ggccgatttt ttcagcggta acgtttcgag
     9661 acggtttttc agcaacacca ggctttcgcg cgccacttca cgcgcttctt tacggtgcag
     9721 gcggctttcg gcattggtat ccaccgggtc agactctttc ggccccaaat ggctgtatgg
     9781 gtcgttaaac aatcccatat catatttaac gttcagtaca tggcgggcag catcgtccag
     9841 ctcttccatc gtcactttgc cggatttgat caacccaggc agatacttcg agtaatactc
     9901 gtcgctcata ctcatgttga ttccggattt cagcgccacg cgcaccgcat cttccgggtc
     9961 tgccgccgtg ccatgtttaa tcagctcttt gattgcaccg tgatcggaaa cggtgatgcc
    10021 tttaaagccc cactggtcgc gcagaacatc tttcagcagc caggaatcgg aggtggctgg
    10081 cgtgccgttc agcgagttca gcgccaccat caccgcgccg ctgcctgcgt ccagccccgc
    10141 tttgtacggc ggcatataat cattaaacag gcgctgcgga ctcatatcga cggtgttgta
    10201 ctctttaccg ccttctaccg cgccgtatgc ggcaaagtgt ttgacgctgg tcatcaccga
    10261 gtagcgatct gccgggcttt taccctgcat cgcttccacc atggttttac ccattgttga
    10321 ggtgagatac gtatcttcgc caaaaccttc ggaagcacgt ccccagcgcg gatcgcgcga
    10381 gacatcgacc atcggtgccc aggtcatatt caggccatca tctgccgctt cataagcaga
    10441 gacacgtccg accgttttca ctgcatcgag gttaaaagac gaggccagac cgaggctaat
    10501 cgggaacacc gtgcgctgac cgtgcagcac gtcgtaagca aagaaaagag gaattttcag
    10561 gcggcttaat tccatcacct gatcctgcat ggcgcggata tcctgacggg ttacggtgtt
    10621 gaaaatcgcc ccaacctgac cgtctttgat catctcgcgg atcgcctctt tcgggttatc
    10681 cgggccgacg ctgattaagc gcagctgacc aattttctca tcaactgtca ttttcttaag
    10741 cagttcggtg acgaacgcat cccgcgcttc gggcgttaat ggatggttgc cgaataaatc
    10801 atccgccagt gctggctgca gggccagact caccgcgatt cctactgaac atagccattt
    10861 catatggatt tttcttcctc tgttgcccga cgaggcagcg aaaatggtgc aaaaaccgta
    10921 gtttgccata agcatgatgg agagagaaaa agaatgctca gtttattgtc tgaattttca
    10981 aaatattcac tcgctgaatt gtcatacaag gcgctattct agtttgtgat attttttcgc
    11041 caccacaagg agtggaaaat gtcttccatg acaacaactg ataataaagc ctttttgaat
    11101 gaacttgccc gtctggtcgg tcattcacac ctgctcaccg atcccgcaaa aacggcccgc
    11161 tatcgcaagg gcttccgttc tggtcagggc gacgcgctgg ctgtcgtttt ccctggctca
    11221 ctactggaat tgtggcgggt gctgaaagcc tgcgtcaccg ccgacaaaat tattctgatg
    11281 caggctgcca atacaggcct gaccgaagga tcgacgccaa acggtaacga ctatgatcga
    11341 gatgtcgtta tcatcagcac cctgcgtctc gacaagctgc acgttcttgg caagggcgaa
    11401 caggtgctag cctatccggg caccacgctc tattcgctgg aaaaagccct caaaccgctg
    11461 ggacgcgaac cgcactcggt gattggatcg tcgtgtatag gcgcatcggt tatcggcggt
    11521 atttgtaaca actccggcgg ctcgctggtg caacgtggcc cggcgtatac cgaaatgtcg
    11581 ttattcgcgc gtataaatga agacggcaaa ctgacgctgg tgaaccatct ggggattgat
    11641 ctgggcgaaa cgccggagca gatccttagc aagctggatg atgatcgcat caaagatgac
    11701 gatgtgcgtc acgatggtcg tcacgcccac gattatgact atgtccaccg cgttcgtgat
    11761 attgaagccg acacgcccgc acgttataac gccgatccgg atcggttatt tgaatcttct
    11821 ggttgcgccg ggaagctggc cgtctttgcg gtacgtctag ataccttcga agcggaaaaa
    11881 aatcagcagg tgttttatat cggcaccaac cagccggaag tgctgaccga aatccgccgt
    11941 catattctgg ctaacttcga aaatctgccg gttgccgggg aatatatgca ccgggatatc
    12001 tacgatattg cggaaaaata cggcaaagac accttcctga tgattgataa gttaggcacc
    12061 gacaagatgc cgttcttctt taatctcaag ggacgcaccg atgcgatgct ggagaaagtg
    12121 aaattcttcc gtccgcattt tactgaccgt gcgatgcaaa aattcggtca cctgttcccc
    12181 agccatttac cgccgcgcat gaaaaactgg cgcgataaat acgagcatca tctgctgtta
    12241 aaaatggcgg gcgatggcgt gggcgaagcc aaatcgtggt tggtggatta tttcaaacag
    12301 gccgaaggcg atttctttgt ctgtacgccg gaggaaggca gcaaagcgtt tttacaccgt
    12361 ttcgccgctg cgggcgcagc aattcgttat caggcggtgc attccgatga agtcgaagac
    12421 attctggcgt tggatatcgc tctgcggcgt aacgacaccg agtggtatga gcatttaccg
    12481 ccggagatcg acagccagct ggtgcacaag ctctattacg gccattttat gtgctatgtc
    12541 ttccatcagg attacatagt gaaaaaaggc gtggatgtgc atgcgttaaa agaacagatg
    12601 ctggaactgc tacagcagcg cggcgcgcaa taccctgccg agcataacgt cggtcatttg
    12661 tataaagcac cggagacgtt gcagaagttc tatcgcgaga acgatccgac caacagcatg
    12721 aatccgggaa tcggtaaaac cagtaaacgg aaaaactggc aggaagtgga gtaaaaatta
    12781 cggatggcag agtatcgcca tccgaattca cttaatcgtt ctgtgccgtc tgccccgccg
    12841 ccgccatttg ggcggctttt tgttttttat agctcaacgc tgctgccggc acaggcatca
    12901 ctttaccggt ttcaatccag gtacgcaggc ggctggcatc ggcaaaatgg gtatatttgc
    12961 caaacgcgtc catcactacc agcgccaccg gtttattatt gataaccgta cgcatcacca
    13021 gacaatggcc cgccgcattg gtaaagccgg ttttggttaa ctgaatattc cagttatcgc
    13081 gatacaccag atgattagta ttgcggaacg gcagcgtata cgtcgggtta gagaaggttg
    13141 ccatatcttc ccgggtagta cttaactgcc cgatcaacgg atactgtttg ctggcaatga
    13201 gcagtttggt taagtcacgg gcagttgaaa cgttatgcac cgacaatccg gtaggttcaa
    13261 caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc attcattgcc ttaataaagg
    13321 ctttgtaacc accgggataa tggtgcgcaa ggcttgccgc cgcgcggttt tctgaagaca
    13381 tcagcgccag caacagcata tctttacggc tgatttcgct attcagtcgt acgcgcgaat
    13441 agaccccttt catctccggc gtctggctga tatccacttt tagtttttca tccagcggca
    13501 gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct gatagacgca atcggacgca
    13561 ccagatccgg gtggttcgaa tagatcactt tgttggtatt cagatcaaca atcatcgcgc
    13621 taccggaggc aatttccggt tgtgaagcgg tggtagcggc tgccgttttc gcaacggcct
    13681 gcggtgcaaa aggcacagcc agcatcaggg ccaggctaaa taaagaaact cgaaatttcg
    13741 gcatgatgag cattcagata gtggttcacg cgcacgggtt gcgcaccgcc ggagtaagga
    13801 tttactgagg ctagcgacgc catcataacg agcaaaaagt gcgatcgtca aaggagaatc
    13861 gtgaggaaat gctgcattgc tgacatttac gccagcaatg caacgtcaaa gaactttcta
    13921 gaacaaacga taaccgtagc cccatagtat aacggttagg gcgagcagca cttccagtac
    13981 cagcacgcca atcgccagcg tcgaactaga gaagctaagg ccttcctctt tgttgatatt
    14041 caggaagctc ggaatatcaa ggtaaagcag gtagccggtg taaaacagcg ccaccgtgcc
    14101 gaccagcgca cacaaccaga ccagtggata aagcgccacc agaccgctta aaaacagcgg
    14161 agttgcaacg tagcccgcga agaccatgca gtgcgcaagt gacggacgct gcggataatt
    14221 acgcgccatc caccagatga cccgccccat caccgcgacc ccagccagca taacgccgta
    14281 aaacaagaca gccagcgcca gtccggtaaa ccaggataac ttcaggatag tgccatcgcc
    14341 aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa atcaccggaa tcgccgccat
    14401 cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg ttttcgcgat taatcacctg
    14461 catttcacga tcgggatggg aaaacagtcc ccagacatgg ctcataccgc ccccttgttg
    14521 tgagttcatg aacctgacag ttcaagtata agtcagcttg tgattatttt ttgttcgccc
    14581 atgtaatttt cactgtctga tgaacgtcct ttttcagcgt gaatgattca cagggtgtat
    14641 gcttactggc aaccaaaggg agacagactg gcctatggat ctcaatacac ttatctcaca
    14701 atatggttat gccgcgctgg tgatcggtag cctggcggaa ggtgaaaccg tgactttgct
    14761 gggaggcgtt gcggcgcatc aggggctatt aaagttcccg ctggtggtac tttctgtggc
    14821 gcttggcggc atgattggcg accaggtgct ctatctgtgc gggcggcggt ttggcggcaa
    14881 gctgttacgc cgtttctcga aacatcagga taaaattgag cgggcgcaga aacttatcca
    14941 acgccatccg tatctgtttg tcattggtac gcgctttatg tatggctttc gggtgattgg
    15001 cccgacgctg attggtgcca gccagctgcc gccgaaaatc tttctgccgc tgaatattct
    15061 cggcgcattt gcctgggcgt tgatttttac cactattggt tacgctggtg gtcaggtgat
    15121 tgcgccgtgg ttgcacactc tcgaccagca tttgaagcac tgggtctggt tgattctggt
    15181 cgtggttctg gtggtaggcg tgcgctggtg gctgaaacga cgcgggaaga aaaagccgga
    15241 taatcaggcg taaaaccatt gccctggata aggcgttcgc gccgcatccg acatccgggg
    15301 caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct accaaaattt tacccattga
    15361 aataaccacg cacctcactg cgctattctg ggttgaactg cggattcgcc aacataaagc
    15421 cgccatccac tatcaacgac tgcccggtgg tgtaatttgc gccctctgag cacagccacg
    15481 ctaccaggct ggcaatctca tgcgttgcgc caaaacgccg cagaggaatc gaaggctccg
    15541 cgtcgggctt cacgtagctg tcatccatgc cattcattgg cgtggcgatc gccccaggcg
    15601 cgactgcgtt caccaaaatc ttatgcctga ccagctccag cgccatcgtt ttggttaacc
    15661 cgccgagcgc atgtttagcg gctgtgtaag cgctggcatc cggcagcggc gtatgttcat
    15721 gtaccgacgt aatgttgatg atgcgaccgc cctgcccttg tttcaccatc tggcgggccg
    15781 caatttgtga gcataaaaat gcaccatcga catcaacggt aaaaatcttg cgccactcat
    15841 cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc cgcattattc accagcacat
    15901 caatacgccc cagccgttga atgagtttct ccagcgcctg tgcaccttct ggcagtttac
    15961 cgagatccag ctgcacaatc tccgcacgta cgccgtggct aactacctca cgcgcggtat
    16021 cttttgcccc ttcttcatct gagtgccagg taatgccaat atcaaacccc tgctgcgcca
    16081 gtaataacgc gcactctttg ccgatccccg aatcggaggc ggtaataatc gcaacctgtg
    16141 ccatcgagtt ctccacttaa cgctgaataa acgttaagta tagaaggcgc atatcatcag
    16201 cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg aagcatccag ccactgccct
    16261 tgcaataaca ggccattggc ccgctcacgc agcgcgggga ttctggcttc gctgacgcgg
    16321 gaaccagcaa tgatgcccgc gttaaagcgc gcctgcgcaa gacctaccac acgcaaggca
    16381 tcgcgctcaa tttgcgcctg atgctggttt ttctccgcaa gtgtctgaac ctgactggct
    16441 gcccgcgcca cgtcattcac cgcttcaacc accgctttgt tgtagctggc gatagacaag
    16501 ttgctttcgg cttttgcgat atcgagattg gcgttaagac gaccactatc gaaaatgggt
    16561 agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat gacggaacag atcgcttaag
    16621 tgcaacgcat cctgttgcag gaaggccatc aggttgatgt caggataaaa tgccgctttt
    16681 gccgcatcaa tggtgcttag cgatgactca acgtaccagt gcgccgcctg caaatctgcc
    16741 cgccgggcca gtaaggagca ccccagttca tcaggaagct ggcttgccac tttcggcaac
    16801 gcgaccggtt taagcttcaa tgactttgtc tggttatttg taagtgcgct taaccgtgcc
    16861 tcaataattt tcattttccc cgcgacatcg ttgagctgct gccgggtttt gctggcatta
    16921 atatcggttt ccacaccttc aactgaagaa gtaatcccgt tctgatatag ctggcgatcg
    16981 gtcgcgataa tggtgttctg ctctttttct atttgctgca agaccgtgtt taacgccgcc
    17041 tgggtttgcc actcccagta caggcgggct acgctgccag ccagcaattg gcgggtttgc
    17101 tcgcgttccg ccgcccgtgc tttaaccgta cccaggcggg cagtaacctc cgcccgattc
    17161 tttccccaga tatcgagatg ccagcccgcc gttaagccaa aagttccgtt ggtgtaccac
    17221 ggaccggtcg tacctgcggc cggatcgttc agagcaaacg gccccattaa gccttctgcc
    17281 gacatttttt gccgctccat atccgcagaa aagtcgatct gcggaccatc ctgagtagca
    17341 attgccttcg cctgggcttc agctagctga atgcgctgtt cagccacctg catatccggt
    17401 gcgttctgta gtgcattgtt aattaaggaa gtgagttgat tatcgtgata ctccagccac
    17461 cattggctgt ctggccaacc atttttcagc gccgtgggta atgcggtgtc aacttgtgca
    17521 gcgggcgttt gctggcttaa cgcctggcgg gtttcatgca taggcgcaca cccggccagc
    17581 atcagtaaca gcggaaaaca ggcgatggcg ggataaaagg aatcacgatt catgggggaa
    17641 taatcaggta agaaaaggtg cgcggagatt accgtgtgtt gcgatatatt ttttagtttc
    17701 gcgtggcaat acatcagtag caataaaacg acatatccag aaaaatatac actaagtgaa
    17761 tgatatcttc cgatttatct taatcgttta tggataacgg caaagggctt cgttttttcc
    17821 tatacttatt cagcactcac aaataaaggt acgccaatga aaattatact ctgggctgta
    17881 ttgattattt tcctgattgg gctactggtg gtgactggcg tatttaagat gatattttaa
    17941 aattaattaa tgtcatcagg tccgaaaata acgagaatat ttcagtctct catcctgttg
    18001 cgctcctgtc atgtgtattg cttcatata
//
LOCUS       DAZFVO020000100        17997 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFVO020000100 DAZFVO020000000
VERSION     DAZFVO020000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.101"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(57..470)
                     /gene="hns"
                     /locus_tag="ACHKNB_RS16035"
                     /old_locus_tag="pgaptmp_003207"
     CDS             complement(57..470)
                     /gene="hns"
                     /locus_tag="ACHKNB_RS16035"
                     /old_locus_tag="pgaptmp_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HGU4196670.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1075..1692
                     /gene="tdk"
                     /locus_tag="ACHKNB_RS16040"
                     /old_locus_tag="pgaptmp_003208"
     CDS             1075..1692
                     /gene="tdk"
                     /locus_tag="ACHKNB_RS16040"
                     /old_locus_tag="pgaptmp_003208"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGU4196671.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(1978..3306)
                     /gene="insG"
                     /locus_tag="ACHKNB_RS16045"
                     /old_locus_tag="pgaptmp_003209"
     CDS             complement(1978..3306)
                     /gene="insG"
                     /locus_tag="ACHKNB_RS16045"
                     /old_locus_tag="pgaptmp_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HGU4196672.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGRGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(3432..6107)
                     /gene="adhE"
                     /locus_tag="ACHKNB_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /gene_synonym="adhC"
     CDS             complement(3432..6107)
                     /gene="adhE"
                     /locus_tag="ACHKNB_RS16050"
                     /old_locus_tag="pgaptmp_003210"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGU4196673.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            6500..6553
                     /gene="ychE"
                     /locus_tag="ACHKNB_RS16055"
                     /old_locus_tag="pgaptmp_003211"
     CDS             6500..6553
                     /gene="ychE"
                     /locus_tag="ACHKNB_RS16055"
                     /old_locus_tag="pgaptmp_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HGU4196674.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            6584..7231
                     /gene="ychE"
                     /locus_tag="ACHKNB_RS16060"
                     /old_locus_tag="pgaptmp_003212"
     CDS             6584..7231
                     /gene="ychE"
                     /locus_tag="ACHKNB_RS16060"
                     /old_locus_tag="pgaptmp_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HGU4196675.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(7389..7685)
                     /locus_tag="ACHKNB_RS16065"
                     /old_locus_tag="pgaptmp_003213"
     CDS             complement(7389..7685)
                     /locus_tag="ACHKNB_RS16065"
                     /old_locus_tag="pgaptmp_003213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196676.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            7969..9600
                     /gene="oppA"
                     /locus_tag="ACHKNB_RS16070"
                     /old_locus_tag="pgaptmp_003214"
     CDS             7969..9600
                     /gene="oppA"
                     /locus_tag="ACHKNB_RS16070"
                     /old_locus_tag="pgaptmp_003214"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HGU4196677.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9686..10606
                     /gene="oppB"
                     /locus_tag="ACHKNB_RS16075"
                     /old_locus_tag="pgaptmp_003215"
     CDS             9686..10606
                     /gene="oppB"
                     /locus_tag="ACHKNB_RS16075"
                     /old_locus_tag="pgaptmp_003215"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HGU4196678.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            10621..11529
                     /gene="oppC"
                     /locus_tag="ACHKNB_RS16080"
                     /old_locus_tag="pgaptmp_003216"
     CDS             10621..11529
                     /gene="oppC"
                     /locus_tag="ACHKNB_RS16080"
                     /old_locus_tag="pgaptmp_003216"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGU4196679.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            11541..12554
                     /gene="oppD"
                     /locus_tag="ACHKNB_RS16085"
                     /old_locus_tag="pgaptmp_003217"
     CDS             11541..12554
                     /gene="oppD"
                     /locus_tag="ACHKNB_RS16085"
                     /old_locus_tag="pgaptmp_003217"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HGU4196680.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            12551..13555
                     /gene="oppF"
                     /locus_tag="ACHKNB_RS16090"
                     /old_locus_tag="pgaptmp_003218"
     CDS             12551..13555
                     /gene="oppF"
                     /locus_tag="ACHKNB_RS16090"
                     /old_locus_tag="pgaptmp_003218"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HGU4196681.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(13608..13937)
                     /gene="yciU"
                     /locus_tag="ACHKNB_RS16095"
                     /old_locus_tag="pgaptmp_003219"
     CDS             complement(13608..13937)
                     /gene="yciU"
                     /locus_tag="ACHKNB_RS16095"
                     /old_locus_tag="pgaptmp_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HGU4196682.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(13972..15432)
                     /gene="cls"
                     /locus_tag="ACHKNB_RS16100"
                     /old_locus_tag="pgaptmp_003220"
     CDS             complement(13972..15432)
                     /gene="cls"
                     /locus_tag="ACHKNB_RS16100"
                     /old_locus_tag="pgaptmp_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGU4196683.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            15575..15748
                     /gene="yciY"
                     /locus_tag="ACHKNB_RS16105"
                     /old_locus_tag="pgaptmp_003221"
     CDS             15575..15748
                     /gene="yciY"
                     /locus_tag="ACHKNB_RS16105"
                     /old_locus_tag="pgaptmp_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HGU4196684.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(15803..17056)
                     /gene="kch"
                     /locus_tag="ACHKNB_RS16110"
                     /old_locus_tag="pgaptmp_003222"
     CDS             complement(15803..17056)
                     /gene="kch"
                     /locus_tag="ACHKNB_RS16110"
                     /old_locus_tag="pgaptmp_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HGU4196685.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(17355..17651)
                     /gene="yciI"
                     /locus_tag="ACHKNB_RS16115"
                     /old_locus_tag="pgaptmp_003223"
     CDS             complement(17355..17651)
                     /gene="yciI"
                     /locus_tag="ACHKNB_RS16115"
                     /old_locus_tag="pgaptmp_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HGU4196686.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT         4602 a         4184 c         4392 g         4819 t
ORIGIN      
        1 gacaataaaa aatcccgccg ctggcgggat tttaagcaag tgcaatctac aaaagattat
       61 tgcttgatca ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca
      121 gctggagtac ggccttggcc agtccaggtt ttagtttcgc cgttttcgtc aacgtagcta
      181 tattttgccg gacgctgagc acgtttagct ttggtgccag atttaacggc agcaaggcta
      241 ttcagcagtt cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagc
      301 ttacgagtgc gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta
      361 acaacaactt ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc
      421 tgcgcacgaa gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc
      481 tcaaacttat attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca
      541 atagccagga atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct
      601 tttcataaaa ttagccagaa aagacgcggc atatagccct atttacaccg atgatttcgc
      661 agcacgtgag gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc
      721 tgacgacctt tcagtcttct gtattagttg tgtttacgag aattccctat taagcgaatg
      781 atgaaaagta gaacagtcgc aataagagca tggacttagt attgcactat ctcctggagg
      841 tcaacagagg gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca
      901 tttacccgct tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc
      961 ttcgtaaggg agaacgcata tacccacttc tgtgcatact gttgagctga aaaactgacg
     1021 aattatgata aactccagcc aactttattt catatcattg agggcctgtg gctgatggca
     1081 cagctatatt tctactattc cgcaatgaat gcgggtaagt ctacagcatt gttgcaatct
     1141 tcatacaatt accaggaacg cggcatgcgc actgtcgtat atacggcaga aattgatgat
     1201 cgctttggtg ccgggaaagt cagttcgcgt ataggtttgt catcgcctgc aaaattattt
     1261 aaccaaaatt catcattatt tgatgagatt cgtgcggaac atgaacagca ggcaattcat
     1321 tgcgtactgg ttgatgaatg ccagttttta accagacaac aagtatatga attatcggag
     1381 gttgtcgatc aactcgatat acccgtactt tgttatggtt tacgtaccga ttttcgaggt
     1441 gaattattta ttggcagcca atacttactg gcatggtccg acaaactggt tgaattaaaa
     1501 accatctgtt tttgtggccg taaagcaagc atggtgctgc gtcttgatca agcaggcaga
     1561 ccttataacg aaggtgagca ggtggtaatt ggtggtaatg aacgatacgt ttctgtatgc
     1621 cgtaaacact ataaagaggc gttacaagtc ggctcattaa cggctattca ggaaaggcat
     1681 cgccacgatt aataagaatt tctttactga cagggtgagc agggcacttt tatcctgtca
     1741 gttcgtttta cgcacttctt ccgggctata tacccttctc ggcagttttt taacgccgct
     1801 atacgcctca cagggctctt aagcaccgac gttgacttgt gacctgtaaa gtacaatatc
     1861 cctgtgttta ggcgttatac atcgtcgcaa atatgatgaa ggctaatgct gtcggtttat
     1921 ggaaaagttg ctttgggtaa acaaaaaaac ggccccagaa ggggtaatgc cagtcagtta
     1981 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
     2041 tctcgggaag gccctttccc ttcttgtcgg taatttcaca agttgtccca tacttgcaag
     2101 atcgtgcatc agctccggta tacgtcccgg tgaagcgccc tgcaatgtca tcagcattct
     2161 catcaccatt ccgcatgatt ctgagaaact cagttgattc ggccagtaac ctttcagatg
     2221 ttccgccatt ttaatcatct gatatctcac cagattataa gccagtaaga caccccacag
     2281 ctcttgctcc acaagctccg gctttttact tctcagcgtc agcctgctca gttgcatcgt
     2341 ctgttttatc tccctgtatc ccagttcgat ttcccagcga tgactgtaca gatccgccat
     2401 ttctcctccg gggaagcgca tggcgtccgt catcgacgtc agcagatggc agacttttcc
     2461 tttgcgcgtc acggtcagca ggcgggctgt cacctcattt ccccgtcccg gccacttttt
     2521 tcgtgcctgc gggctggttt tcagcttcac cagatgatcg cctttaccca gttttctgat
     2581 ctcttcatat tgcgctccct ttctgagagg gatcatccag tggcggtgtt ctcccgccag
     2641 gctccaggca tttaacagtc ccagtgagta ataacctttg tccattaacg tcagagtgtt
     2701 atcgccggtt tgttctataa gttgctcagc aagctcattt tcgctgttct tcatcgtgcc
     2761 gaaggctgca gccgtcagca gatggctggt cagttccatc tggcagacca ttttgacctg
     2821 cgggtagagc gccgggttcc cggcatgtgt ctggcggggg aaggctgcat cgttctctgg
     2881 tgtatccggt gtgcgccaga acacaccatc gatggccagc agggtcaggc cgcaccattg
     2941 cggatgcggc gtggcgttat gccagagctg cgctgttttc gtgaacacgc ggcggacagc
     3001 ctcacttccc aggcgctggc gggcctgaat aacggcactg ggggcaacga aggggcgatt
     3061 gcccggcagc atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag
     3121 cgccatgcca acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt
     3181 tacagtacct gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag
     3241 agaagtcagt gggttacgca gagaatcgta acgggatacc aaatcaaggg cctgtccaat
     3301 gtgcataaaa aaatccggaa acgagtgagc gtttccggat tcttacacag ccactggatc
     3361 ggtcaactga tccttaactg atcggcatta ccccagaagg ggccgtttat attgccagac
     3421 agcgctactg attaagcgga ttttttcgct tttttctcag ctttagccgg agcggcttct
     3481 tttttcgctg cagtttcacc ttctacataa tcacgaccgt agtaggtatc cagcaggatc
     3541 tgtttcagct cggagatcag cgggtaacgc gggttagcgc cggtgcactg gtcatcgaac
     3601 gcatcttcag acagtttatc cacgttcgcc aggaagtctg cttcctgaac gccagcttca
     3661 cggatagatt tcggaatacc cagttcagct ttcagcgttt ccagccatgc cagcagtttc
     3721 tcgatcttag cagcagtacg gtcgcccggt gcgctcagac ccaggtggtc ggcaatttca
     3781 gcataacgac ggcgagcctg cggacggtca tactggctga atgcagtctg cttggtcggg
     3841 ttgtcgttcg cattgtagcg aataacgtta caaatcagca gggcgtttgc cagaccgtgc
     3901 ggaatatgga actgggaacc cagtttgtgc gccattgagt gacatacacc caggaaggcg
     3961 ttcgcaaacg cgatacccgc gatagtcgct gcactgtgaa cacgttcacg cgctaccgga
     4021 tttttagacc cttcgtggta ggacgctggc agatattctt tcagcagttt cagtgcctgc
     4081 agagcctgac catcagagaa ctcagatgcc agtacagaaa cataagcttc catggcgtga
     4141 gttactgcgt ccagaccacc gaaagcacac agggacttcg gcatgtccat aaccaggttg
     4201 gcgtcgacaa tcgccatatc cggagtcagc gcatagtctg ccagcggata tttctgacca
     4261 gtagcgtcgt cagttacaac cgcaaacgga gtgacttcag aacctgtacc agaagtggtg
     4321 gtgacagcga tcattttcgc tttcacgccc attttcggga acttgtagat acgtttacgg
     4381 atatccataa agcgcagcgc cagctcttcg aagtgagttt ccggatgttc gtacataacc
     4441 cacatgatct tcgcggcgtc catcggggaa ccaccaccca gcgcgataat cacgtctggt
     4501 ttgaaggagt ttgccagttc tgcaccttta cgaacgatgc tcagggtcgg gtccgcttct
     4561 acttcgaaga agacttcagt ttcaacgcct gctgctttca gtacggaagt gatctgatca
     4621 gcataaccat tgttgaacag gaagcggtca gtcacgatga gcgcacgttt gtggccatca
     4681 gtaatcactt catccagcgc gattggcagg gagccacggc ggaagtagat agatttcgga
     4741 agtttgtgcc acaacatgtt ttcagctcgc ttagcaacgg ttttcttgtt gatcaggtgt
     4801 ttcggaccaa cgttttcaga gatggagtta ccaccccaag aaccacaacc cagagtcagg
     4861 gaaggtgcga gtttgaagtt atacaggtca ccgataccac cctgagacgc tggggtgtta
     4921 atcaggatac gagccgtttt cattttttga ccgaagtaag aaacgcgagc cggttggtta
     4981 tcctggtcag tgtacaggca agaggtatga ccgataccgc ccatagcaac cagtttctct
     5041 gctttttcta ccgcgtcttc gaaatcttta gcgcggtaca ttgccagagt cggggacagt
     5101 ttttcatgtg cgaacggttc gctttcatca acaacggtca cttcaccgat cagaatcttg
     5161 gtgttttctg gtacagagaa gcctgccagt tcagcaattt tataggctgg ctgaccaacg
     5221 atagccgcgt tcagcgcacc gtttttcagg ataacatcct gaacagcttt cagctcttta
     5281 ccctgcaaca gatagccgcc gtgggttgca aaacgttcac gtacagcgtc ataaacagag
     5341 tcaacaacaa caacagactg ttcagaagca cagattacgc cgttgtcgaa ggttttggac
     5401 atcagtacag atgcaactgc acgtttgata tcagcagttt catcgataac aactggagtg
     5461 ttgcccgcgc ctacaccgat agctggttta ccggagctgt atgcggcttt aaccatgccc
     5521 ggaccaccag tcgcgaggat caggttgatg tctgggtggt gcatcagtgc gttagacagt
     5581 tcaacagaag gttgatcgat ccagccgatc agatctttcg gagcaccggc agcgatagca
     5641 gcctgcagaa cgatatcagc cgctttgttg gtggcatctt ttgcacgcgg gtgcggggag
     5701 aagataatgg cgttacgggt cttcagactg atcagcgatt tgaagatagc agttgaagtc
     5761 gggttagtgg tcggaacgat accgcaaata ataccgattg gttcagcgat agtgatggta
     5821 ccaaaagtgt cgtcttcaga cagaacacca caggtttttt catctttata ggcgttgtag
     5881 atatattcag aagcaaagtg gtttttgatc actttatctt cgacgatacc catgccggat
     5941 tcggcaacgg ccattttcgc gagtgggatt cgagcatctg cagcagccag agcggcggcg
     6001 cggaagattt tgtctacttg ctcttgagtg aaactggcat attcacgctg ggcttttttt
     6061 acacgctcta cgagtgcgtt aagttcagcg acattagtaa cagccataat gctctcctga
     6121 taatgttaaa cttttttagt aaatcatctg ctcgaatacg agagtatagt cagtgcggtg
     6181 atgatttgct taacctatga aaatcaaaag cttactcgcg ctcacactca ctgtgattta
     6241 ctaaaagagt ttaaacatta gagttattat ctctaatgcg tcacttccag gtggcgtaag
     6301 caagattact cacttctggg tactgattac gtgatccaaa tcaaattttt gcaaagctga
     6361 cacctttcag catcgctttt cgccattata gctaacagtt aataaattgt agtatgattt
     6421 ggtggctaca ttagcatgtt ttgcacaact agataacaat aacgaatgat agcaatttta
     6481 agtagttagg aggtgaaaaa tgctgtcaaa aggcgtattg tcagcgcgtc ttttcaacct
     6541 tatttatggc taacattatc cggcttttgc ttcggagcta accgtgattc agaccttttt
     6601 tgattttccc gtttacttca aatttttcat cgggttattt gcgctggtca acccggtagg
     6661 gattattccc gtctttatca gcatgaccag ttatcagaca gcggcagcgc gaaacaaaac
     6721 taaccttaca gccaacctgt ctgtggccat tatcttgtgg atctcgcttt ttctcggcga
     6781 cacgattctg caactttttg gtatatcaat tgattcgttc cgtatcgccg ggggcatcct
     6841 ggtggtgacg atagcgatgt cgatgatcag cggcaagctt ggcgaggata aacagaacaa
     6901 gcaagaaaaa tcagaaaccg cggtacgtga aagcattggt gtggtgccat tggcgttgcc
     6961 gttgatggcg gggccagggg cgatcagttc taccatcgtc tggggtacgc gttatcacag
     7021 cattagctat ctgtttggtt tctttgtggc tattgcattg ttcgctttat gttgctgggg
     7081 attgttccgc atggcaccgt ggctggtacg ggttttacgc cagaccggca tcaacgtgat
     7141 tacgcgtatt atggggctat tgctgatggc attggggatt gaatttatcg ttactggtat
     7201 taaggggatt ttccccggcc tgcttaatta attcctttca aatgaaacgg agctgccatg
     7261 ctccgtttac ttcgtcatta tttttacttt gttcccgcgc agttatcaaa agcaaaagga
     7321 ataggtaaaa atattcttct caaattacag ttggttataa ggatttcctt aactgcttct
     7381 cctcaccatc atgttatttt cgccacatca taatcctggg cttgctgaag aataattgaa
     7441 atgatattag taattccact gcctttggta gaggaaagtg ctaaataata atcaattgtt
     7501 aaattattgt gcatttcact actggaactg taatcagaaa agatagacat gcttagccaa
     7561 tctctatttg attgaattga aagatgtttg ttaaggcatg gatgcaagct atagattctg
     7621 atacggtcaa taaaagagaa ttgcttaaca attttgcaaa atgtattggc gagtaagaac
     7681 cgcatttggt actttccggg caaccgccag acgattcttt attggtaatg agaataatta
     7741 acaattaaag agcgtcgcga aagaataatg tgtctcgaca ggggagacac agtacgaatc
     7801 gacataaggt gatcgtctga atcaccagaa taaataaagt cggtgatagc aatccgtaac
     7861 gataaagtaa cctgacagca gaaagtctcc gagcctgtgc agggtcccaa tccgggatta
     7921 cacatgctgg ttaataccag taattataat gagggagtcc aaaaaacaat gaccaacatc
     7981 accaagagaa gtttagtagc agctggcgtt ctggctgcgc taatggcagg gaatgtcgcg
     8041 ctggcagctg atgtacccgc aggcgtcaca ctggcggaaa aacaaacgct ggtacgtaac
     8101 aatggttcag aagttcagtc attagatccg cacaaaattg aaggtgttcc ggagtctaat
     8161 atcagccgag acctgtttga aggcttactg gtcagcgatc ttgacggtca tccggcacct
     8221 ggcgtcgctg aatcctggga taataaagac gcgaaagtct ggaccttcca tttgcgtaaa
     8281 gatgcgaaat ggtctgatgg tacgccagtc acagcacaag actttgtgta tagctggcaa
     8341 cgttctgttg atccgaacac tgcttctccg tatgccagtt atctgcaata tgggcatatc
     8401 gccggtattg atgaaattct tgaagggaaa aaaccgatta ccgatctcgg cgtgaaagct
     8461 attgatgatc acacattaga agtcacctta agtgaacccg ttccgtactt ctataaatta
     8521 cttgttcacc catcaacttc accggtgcca aaagccgcta tcgagaaatt cggcgaaaaa
     8581 tggacccagc ctggtaatat cgtcaccaac ggtgcctata ccttaaaaga ttgggtcgta
     8641 aacgaacgaa tcgttcttga acgcagcccg acctactgga acaacgcgaa aaccgttatt
     8701 aaccaggtaa cctatttgcc tattgcttct gaagttaccg atgtcaaccg ctaccgtagt
     8761 ggtgaaatcg acatgactta taacaacatg ccgatcgaat tgttccagaa gctgaaaaaa
     8821 gagatcccgg acgaagttca cgttgatcca tacctgtgca cttactatta cgaaattaac
     8881 aaccagaaac cgccattcaa cgatgtgcgt gtgcgtaccg cactgaaact aggtatggac
     8941 cgcgatatca ttgttaataa agtgaaagcg cagggcgaca tgcccgccta tggttacact
     9001 ccaccgtata ctgatggcgc aaaattgact cagccagaat ggtttggctg gagccaggaa
     9061 aaacgtaacg aagaagcgaa aaaactgctg gctgaagcgg gttataccgc agacaaaccg
     9121 ttgaccatca acctgctgta taacacctcc gatctgcata aaaagctggc gattgctgcc
     9181 tcttcattgt ggaagaaaaa cattggtgta aacgtcaaac tggttaacca ggagtggaaa
     9241 acgttcctcg acacccgtca ccagggcact tttgatgtgg cccgtgcagg ctggtgtgca
     9301 gactacaacg aaccaacttc cttcctgaac accatgctgt cgaacagctc gatgaatacc
     9361 gcgcattata agagcccggc ctttgacagc attatggcgg aaacgctgaa agtgactgac
     9421 gaggcgcagc gcacagctct gtacactaaa gcagaacaac agctggataa ggattcggcc
     9481 attgttcctg tttattacta cgtgaatgcg cgtctggtga aaccgtgggt tggtggctat
     9541 accggcaaag atccgctgga taatacctac acccggaata tgtacattgt gaagcactaa
     9601 tggcaatacg tggggcagga gtgtcctgct ccacggtgtc tgatttttat cgcattacag
     9661 aaggcacagg ccagaaggta gggcaatgtt aaaatttatt ctacgtcgct gtctggaagc
     9721 gattccgacg ctatttattc ttattactat ttcgttcttt atgatgcgcc tcgcgccggg
     9781 aagccctttt accggcgaac gtactttacc gccggaagtg atggccaata tcgaagcgaa
     9841 atatcatctt aatgatccga tcatgacaca gtatttcagt tacctgaaac aactggcgca
     9901 cggtgatttc ggtccatcgt ttaaatataa agattattcg gtcaatgacc tggttgcatc
     9961 cagttttccc gtttctgcca aactgggagc cgcagcattt ttccttgcgg taatactggg
    10021 tgttagtgct ggcgttattg ccgcattaaa acaaaacacc aaatgggact ataccgtgat
    10081 ggggctggca atgaccgggg ttgttatccc cagttttgtg gttgcgccat tattagtcat
    10141 gatatttgcg atcattttgc attggctgcc gggcggtggc tggaatggtg gggctcttaa
    10201 attcatgata ttgccgatgg tggcgttgtc actcgcttat atcgccagta ttgcgcgtat
    10261 tacccgtggc tctatgattg aagtattaca ctccaatttt attcgtactg cccgggcgaa
    10321 agggttacct atgcggcgga tcattttacg tcacgcatta aaacctgctc tgttacccgt
    10381 gctctcttat atgggccccg catttgtcgg cattattacc ggttctatgg ttatcgaaac
    10441 catttatggt ttgccgggga ttggacaact gttcgttaat ggcgcattga accgtgacta
    10501 ttccttagtg ttaagcctga ccatcctggt tggtgcttta accattttgt ttaatgccat
    10561 tgtcgatgtg ctatatgcgg ttatcgaccc gaaaatccgt tactgatact ggagctcgcg
    10621 atgatgttaa gtaagaaaaa cagcgagacg ctggaaaatt tcagtgaaaa gctggaggtc
    10681 gaagggcgca gcttgtggca ggacgcacgt cgacgtttta tgcataaccg tgcggcggtt
    10741 gccagtctga tagtgctggt gctgatcgcg ttatttgtaa tcctggcacc gatgctttcc
    10801 cagtttgcct atgacgatac tgactgggcg atgatgtcca gcgccccgga tatggagtcc
    10861 ggtcactact ttggtactga ctcatccggt cgcgacctgc ttgtgcgcgt tgcgattggc
    10921 gggcgtatct cactcatggt cggtgttgct gcggcactgg tggctgtggt cgtcgggaca
    10981 ctttatggtt cgctttccgg ttatctgggc ggtaaagtgg attcggtaat gatgcgtctg
    11041 ctggaaatcc tcaactcctt cccattcatg ttcttcgtca ttttgctggt gacctttttc
    11101 ggtcaaaaca tcctgctgat tttcgtggcg ataggcatgg tttcctggct ggatatggct
    11161 cgtattgtgc gtgggcaaac cctgagtctg aagcgcaaag agtttattga ggcggcacaa
    11221 gttggcggtg tatcgacatc gggcattgtt attcgccaca ttgtgccgaa tgtactcggt
    11281 gtggtggtgg tctacgcatc acttctggtg cccagcatga tcctctttga atctttcctt
    11341 agcttcctgg ggttgggtac gcaagagccg ttaagcagct ggggtgcatt gctgagtgat
    11401 ggcgcgaact cgatggaagt ctctccatgg ttattgttgt tcccagcggg attcctcgtg
    11461 gtgacgctgt tttgtttcaa ctttatcggc gatggcttgc gtgatgccct cgacccgaaa
    11521 gatcgttaag gagtgcagcc atgagcgtaa ttgaaactgc aactgtgccg ctcgcacaac
    11581 aacaggctga cgcactgctg aacgtgaaag atttgcgtgt cacctttagt accccggacg
    11641 gcgacgtcac ggcggtcaat gatttgaatt tttccctacg tgccggagaa acgctgggta
    11701 ttgtaggtga gtctggttcg ggtaaatcgc aaactgcatt tgcgttgatg ggcctgttgg
    11761 ctgccaacgg acgtattggc ggatcggcaa ccttcaatgg gcgtgaaatc ctcaatttgc
    11821 cagagcatga actcaataaa ctgcgcgctg aacaaatctc aatgattttt caggacccaa
    11881 tgacttcgtt gaatccctat atgcgcgtcg gtgagcagtt aatggaagtg ctgatgctgc
    11941 ataagaacat gagcaaagct gaggcgtttg aagagtcggt gcggatgctc gatgcggtaa
    12001 aaatgccgga agcgcgtaaa cgcatgaaaa tgtacccgca cgaattttct ggcggcatgc
    12061 gtcagcgagt catgattgcg atggcattgc tatgtcgacc taagctactg attgcggatg
    12121 aacccactac tgcgctggac gtcaccgtac aggcgcagat catgacgcta ttaaatgaac
    12181 tgaagcggga atttaatacc gccatcatta tgatcaccca cgatcttggc gtggtggcgg
    12241 ggatctgtga caaagtgctg gtaatgtacg ccgggcgcac gatggaatat ggcaatgcgc
    12301 gcgatgtctt ttatcaaccc gttcatcctt attctatcgg tttgctcaac gcggtgccgc
    12361 gtcttgatgc ggaaggtgaa acaatgttga ccatcccggg taatccgcca aacttgctgc
    12421 gattaccgaa aggttgcccg ttccagccac gttgtccaca tgcgatggaa atttgtagta
    12481 gcgcaccgcc gctggaagag tttacgcctg gccgtctgcg tgcttgcttt aaaccggtgg
    12541 aggaactgtt atgaatgctg taactgaagg aagaaaagtc ctccttgaaa tcgccgatct
    12601 taaagtgcac tttgaaatca aagatggcaa acagtggttc tggcaaccgc cgaaaacgct
    12661 caaagccgtc gatggtgtca ctcttcgcct gtatgaaggg gaaacattag gtgtggtagg
    12721 ggaatcggga tgcggtaagt ccacctttgc tcgcgccatc atcggtttgg tcaaggcgac
    12781 cgacggtcat gttgcctggt taggtaaaga gttgctgggc atgaagcccg atgaatggcg
    12841 tgccgttcgc agtgatattc agatgatttt ccaggatccg ttggcatcgc taaacccgcg
    12901 tatgaccatc ggcgagatca tcgctgaacc actgcgtact tatcatccga aaatgtcacg
    12961 ccaggaagtt cgcgagcgcg tgaaggcgat gatgctgaaa gtcgggttat tgcctaacct
    13021 gattaaccgc tatccgcatg agttctccgg tgggcagtgc cagcgtatcg ggattgctcg
    13081 tgctcttatt cttgaaccga agctgattat ctgcgatgag ccggtttcgg cgctggacgt
    13141 gtcaattcag gcgcaggtgg tcaacctgct ccagcagctg caacgtgaga tgggattgtc
    13201 attaattttt atcgctcatg acctggccgt ggtaaaacac atttccgatc gtgtgttggt
    13261 gatgtatctc ggccatgcgg tagaactggg gacctatgat gaggtctacc acaatccact
    13321 acatccttac accagggcat tgatgtcggc agtccccata cctgatccgg atctggagaa
    13381 gaacaaaacc atccagttac tggaagggga attaccgtcg ccgatcaacc cgccttccgg
    13441 ttgtgttttc cgtacccgtt gcccgattgc cggtccggag tgcgccaaaa cacgtcctgt
    13501 tctggagggg agtttcagac acgccgtttc ttgcctgaaa gtcgatccgc tttaaacaca
    13561 ataagggctg acagttgtca gccctttttc acgctaaaag cgattattta ttcccgccag
    13621 atgatatggc aaagtttgtg atctttttcg cggcataaca gaatgcgggc gaaaacatcg
    13681 ttgatttcac catcttcact gtccgccaga ccaatcacca cttcggcaaa aaagtctggg
    13741 ttcaaatcga aatcaacatg ttcctgccag tcttccgccg gatcgaataa ctccgcgccg
    13801 ccgcgctctt caaactgaag attgaacagc agaacatcag ctggatcgag attgtcggca
    13861 gccagttcga gaaaaatatc gtaggcctgc tcaagcgttt catcttcagt caggcgattg
    13921 tttagatcca tatccatagt cactacctgt ttaacctctg ttggcgacgt tttacagcaa
    13981 cggactgaag aagtaaaaca gtcgctcggc gacacgttgc cataatggac gttttagcca
    14041 taaacgggca tcgagcagac gtgaacgcga aatataatcg tcctgaacgg cggcaaggtc
    14101 tgcaccaaaa cctttatcgt cgattgccag ggtaatctcg aaattcagcc acagactccg
    14161 catatcaagg ttaacggtgc caaccagact tagttcgcca tcgaccagca cactcttggt
    14221 atgcagtaac ccgccttcaa actgataaat tttaacccca gcagcaagaa gttccgtaaa
    14281 gaatgcgcga ctggcccagc cgaccagcat cgagtcattt tttcgcggaa ggataatact
    14341 gacatccacc ccgcgctgcg ccgccgtgca aatcgcatga agtaaatcat cgcttggcac
    14401 aaagtagggc gtggtcatga tcaaatattc acgcgccgaa taagccgcag tcaataatgc
    14461 ctggtgaatg agatcttccg gaaagccggg gccagaagca attgtgtgaa tggtgtgacc
    14521 gctggcctgt tcaaacggca taatattgac atctggtggt ggcggcagaa tacgttttcc
    14581 ggtttcaatc tcccagtcgc aggaataaat aatccccatc gcggtggcga tggggccttc
    14641 catacgcgcc atcagatcaa tccattgccc tacgcccgca tcttgtttga agtagcgagg
    14701 atcgaccata ttcatgctgc cggtgtacgc gatgtaatta tcgatcatga tcatcttgcg
    14761 atgttggcgc aggtccatac ggcgtaaaaa cacacgcatc agattgacct ttaaggcttc
    14821 gaccacttca ataccggcat tacgcattag ctcgggccac gggctgcgga aaaaagccac
    14881 actcccggcg gagtcgagca tcaatcggca atgaatacca cgccgtgcag ccgccattaa
    14941 tgattcagcc acctggtccg ccatgccgcc gggctgccag atataaaaca ccatctcaat
    15001 attatggcgc gcgagctgga tgtcgcggat taacgcctgc atcacatcat ctgactcggt
    15061 catcagttgt agctgattcc ctttgacccc agcgatcccc tgacgacgct cgcaaagctt
    15121 gaataatggc gcagcgacac tgctattttc ttcggcgaag atatgcttac aggctttaag
    15181 gtcgttaagc cattttgcgg tggaaggcca catcgctctg gcgcgctcag cgcggcgttt
    15241 gcctaaatgg agctcgccaa cggcaagata ggcaataatt ccgactaacg gcagaatgta
    15301 aataatcaac agccaggcca tcgcggaggg aactgcgcgt cgtttcatta gaatgcgtaa
    15361 agttacgcct gcaatgagca accagtatcc cagaatggcc aaccaactca ccaacgtata
    15421 aacggttgtc atagattaga aatccttttg aaagcgcatt gttatgagtt tacgcatatg
    15481 gattcatctg gcaaataaaa acgcgggaaa agcactggtc agcgtcgggg gtgggggtat
    15541 aatgaccatt ctgttattgc atagagtagt taacatgaag cggagtagaa cggaagtggg
    15601 gcgctggcgc atgcagcgtc aggctagccg acgtaaatcg cgttggcttg aggggcaatc
    15661 gcgccgaaat atgcgtatcc acagcatcag gaagtgcatt ctaaacaaac agcgtaactc
    15721 gttattgttt gcgatctaca atatctaaat atactgggca ccattgcggt gcccaataag
    15781 tttactcttt cgtctttaaa ctctattttt gcactgattc tttactgtca gctttcgttt
    15841 cttgttcatc gttatcgctg aaaatgccat gaccggagtt caacaacatt gaaacgagca
    15901 tatcattatt aatctcttca ccatttaaaa ctcgcgccag aatttcgctg ccaaacagtt
    15961 gcggtgagag aatgatatcc ggatgtacca tcttaatctt attcaggttt ttgctatcac
    16021 tgacggcgag aactgttttg acatcactgc tcatatcttt tgccgagagt acaacaaacg
    16081 cgttatctgc atcgttatca ctcagcgcca gaatggcccg gcaacgatcg attcccgctt
    16141 tctttaatac tgaactgtca ttactgtcac cggggataac atcagcgtta tcgcctaagc
    16201 gttgctcaag ttgcttgata tcatcttcag gcaagttgct gataaccgtt acgttttgtc
    16261 cgcgttgatt cagttgcagg atcgtattga tggcgagaat cgaatgtccg caaacaataa
    16321 aatgatcttt acgatgcatt gtatgattgt ttccttttac aagtttgttg aatcccccgc
    16381 ggataagcgg accaaaaatt gaggtcatag atgtggcaaa aacggtaatg ccggaaataa
    16441 tgaccgaaat agtgaacaat cgtgctgatt cagaaacagg gacaatatcg ccatagccga
    16501 cggttgacat ggtttctatc gaaaaataga acgcggtcat caaactttct attcgcggat
    16561 taaaaccttc gcttaaataa agcgcaccgt aggttgagta aaacagtaat gtcgtgaaac
    16621 taataaatgc aaaaattgtc ccggctgcgg cgctactgtg ggagaagtct ttgcgcagta
    16681 tcagcaaaaa caccagcgta aaaatgcaaa atccaatact aaatttcagc cagggataaa
    16741 aatgcagggt gtaaattagc gctatcaaca acaaaatgat actgattgcc catgcgagct
    16801 ttgcccggaa cagtaagcca atggagttca ggacaagaaa gacgccgagc ataaatagtg
    16861 gggcgtttgc cagcaaggag agatccaact ctgaaaatgc tttgatatgg aaaatatcaa
    16921 gcaaattgac cgacatacca tagattgttt taaaaataag caatccattt aaaaagacgg
    16981 ccagcgcgag aatgtcgtgc cgtagggtca cccataaatt tgttgctgtt tgtttgaatg
    17041 tagcccagtg actcactgat acctctgtaa ccacaatttt gtttaattat cttagcgtgc
    17101 acattttctg aacatacatg cagcgcgtga atgtgttaaa caatagtaga tgactttatc
    17161 ggcagtaatc aaaggttttg agcgagcaac tctgcgtcag gaaagggcat tttcctgaat
    17221 atgttcacat catataaaga aatacagata aagttattat ctgcttgtgg tggtgaatgc
    17281 actgaccggc tataaggaaa ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa
    17341 tattgaatct ctattcagaa cactttctta aatggtttca ctgaaacgtg ttcatagact
    17401 cctgccgcta cgtacgggtc agcatcggcc caggcctgag ctgcttccag cgattcaaat
    17461 tcagcaataa cggttgagcc agtaaatccc gcagcccctg gatcgttact gtctaccgct
    17521 ggcattggac cagctgtcaa caaacgacct tcatcatgca gtaactgtaa acgtgctaaa
    17581 tgtgccggac gaacggaaag gcgtttttcg aagctatcag ctttgtcttg agcgtaaata
    17641 acatacaaca cgggcacaac tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg
    17701 gcgaagatct gcaacggaaa gatgatgtct ttgttaagac catgcataaa gtaaggtcaa
    17761 ttacgcccaa aatgacattt tcactgatcc tgatcgtctt gccttattga atatgattgc
    17821 tatttgcatt taaaatcgag acttggtttt tctactgaaa tgattatgac ttcaatgacc
    17881 cttgaatgaa gtctattcgg ttttctaaga gacctgttat taacaggctt tatcaggtgt
    17941 agttgggctt gttccaaccc ctatgactat gcaatctaat aatagatttc caagtat
//
LOCUS       DAZFVO020000101        17967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFVO020000101 DAZFVO020000000
VERSION     DAZFVO020000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.102"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            19..573
                     /gene="ybeT"
                     /locus_tag="ACHKNB_RS16120"
                     /old_locus_tag="pgaptmp_003224"
     CDS             19..573
                     /gene="ybeT"
                     /locus_tag="ACHKNB_RS16120"
                     /old_locus_tag="pgaptmp_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGU4196687.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLRVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /gene="djlB"
                     /locus_tag="ACHKNB_RS16125"
                     /old_locus_tag="pgaptmp_003225"
     CDS             complement(583..2010)
                     /gene="djlB"
                     /locus_tag="ACHKNB_RS16125"
                     /old_locus_tag="pgaptmp_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HGU4196688.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2714)
                     /gene="ybeR"
                     /locus_tag="ACHKNB_RS16130"
                     /old_locus_tag="pgaptmp_003226"
     CDS             complement(2007..2714)
                     /gene="ybeR"
                     /locus_tag="ACHKNB_RS16130"
                     /old_locus_tag="pgaptmp_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HGU4196689.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWHSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHKNB_RS16135"
                     /old_locus_tag="pgaptmp_003227"
     CDS             2878..3855
                     /gene="ybeQ"
                     /locus_tag="ACHKNB_RS16135"
                     /old_locus_tag="pgaptmp_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HGU4196690.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3925..4407)
                     /gene="ybeL"
                     /locus_tag="ACHKNB_RS16140"
                     /old_locus_tag="pgaptmp_003228"
     CDS             complement(3925..4407)
                     /gene="ybeL"
                     /locus_tag="ACHKNB_RS16140"
                     /old_locus_tag="pgaptmp_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HGU4196691.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4642..7224
                     /gene="leuS"
                     /locus_tag="ACHKNB_RS16145"
                     /old_locus_tag="pgaptmp_003229"
     CDS             4642..7224
                     /gene="leuS"
                     /locus_tag="ACHKNB_RS16145"
                     /old_locus_tag="pgaptmp_003229"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGU4196692.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7239..7820
                     /gene="lptE"
                     /locus_tag="ACHKNB_RS16150"
                     /old_locus_tag="pgaptmp_003230"
     CDS             7239..7820
                     /gene="lptE"
                     /locus_tag="ACHKNB_RS16150"
                     /old_locus_tag="pgaptmp_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HGU4196693.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7820..8851
                     /gene="holA"
                     /locus_tag="ACHKNB_RS16155"
                     /old_locus_tag="pgaptmp_003231"
     CDS             7820..8851
                     /gene="holA"
                     /locus_tag="ACHKNB_RS16155"
                     /old_locus_tag="pgaptmp_003231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGU4196694.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8853..9494
                     /gene="nadD"
                     /locus_tag="ACHKNB_RS16160"
                     /old_locus_tag="pgaptmp_003232"
     CDS             8853..9494
                     /gene="nadD"
                     /locus_tag="ACHKNB_RS16160"
                     /old_locus_tag="pgaptmp_003232"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGU4196695.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9518..10129
                     /gene="cobC"
                     /locus_tag="ACHKNB_RS16165"
                     /old_locus_tag="pgaptmp_003233"
     CDS             9518..10129
                     /gene="cobC"
                     /locus_tag="ACHKNB_RS16165"
                     /old_locus_tag="pgaptmp_003233"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HGU4196696.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHKNB_RS16170"
                     /old_locus_tag="pgaptmp_003234"
     CDS             10389..10706
                     /gene="rsfS"
                     /locus_tag="ACHKNB_RS16170"
                     /old_locus_tag="pgaptmp_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGU4196697.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHKNB_RS16175"
                     /old_locus_tag="pgaptmp_003235"
     CDS             10710..11177
                     /gene="rlmH"
                     /locus_tag="ACHKNB_RS16175"
                     /old_locus_tag="pgaptmp_003235"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGU4196698.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHKNB_RS16180"
                     /old_locus_tag="pgaptmp_003236"
     CDS             11208..13109
                     /gene="mrdA"
                     /locus_tag="ACHKNB_RS16180"
                     /old_locus_tag="pgaptmp_003236"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HGU4196699.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHKNB_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /gene_synonym="rodA"
     CDS             13112..14224
                     /gene="mrdB"
                     /locus_tag="ACHKNB_RS16185"
                     /old_locus_tag="pgaptmp_003237"
                     /gene_synonym="rodA"
                     /EC_number="2.4.99.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HGU4196700.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHKNB_RS16190"
                     /old_locus_tag="pgaptmp_003238"
     CDS             14235..15323
                     /gene="rlpA"
                     /locus_tag="ACHKNB_RS16190"
                     /old_locus_tag="pgaptmp_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HGU4196701.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPIGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15462..16673
                     /gene="dacA"
                     /locus_tag="ACHKNB_RS16195"
                     /old_locus_tag="pgaptmp_003239"
     CDS             15462..16673
                     /gene="dacA"
                     /locus_tag="ACHKNB_RS16195"
                     /old_locus_tag="pgaptmp_003239"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HGU4196702.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHKNB_RS16200"
                     /old_locus_tag="pgaptmp_003240"
     CDS             16784..17047
                     /gene="ybeD"
                     /locus_tag="ACHKNB_RS16200"
                     /old_locus_tag="pgaptmp_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HGU4196703.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17148..17789
                     /gene="lipB"
                     /locus_tag="ACHKNB_RS16205"
                     /old_locus_tag="pgaptmp_003241"
     CDS             17148..17789
                     /gene="lipB"
                     /locus_tag="ACHKNB_RS16205"
                     /old_locus_tag="pgaptmp_003241"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HGU4196704.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4564 a         4502 c         4573 g         4328 t
ORIGIN      
        1 aaatcgaagt attcacatat gaataaaaaa ttaatgtata tattcgcaat atttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacaacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagctcacca ggcagtctac cgttaaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      361 agagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      601 tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      661 acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg gctcgacggt
      721 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttcggttgg
      841 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccaga gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagcgg ttttccctta tcgttaacca gatcacgcgc
     1081 ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc accattgcaa
     1141 cattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccag
     1201 caagctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc agaagttcgg ggatatccag
     1321 gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggatgat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaata tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatttcgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     2041 catgcagaat agtaagccaa tggttgccag gacaaggtag ggtaatacgc attatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat
     2161 tcacgtaata actgcagatt atcatcttcg ggagaatgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacga taataacgta gagcccgtag atgaatgcgc
     2281 gactggatcc atagactttc ttcttcgctc aaccagttat taagtacgcc cattcggctt
     2341 aaaaaaccca tccggacata atcccaggct ttaattcctc cctcaccaca gcattcggcg
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtta
     2461 cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttgggtga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc
     2701 gactccatgt ccatataatt tatccttaaa tattcattaa atgaatatta gccgcattgc
     2761 gaggctcgtt tatttatact tttctgacgc gacataatac gcataaaata aggcttacgc
     2821 aaaaatggaa cacataaaaa tataatatgt tttatctgca accataagga aatgataatg
     2881 attttcacgt caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa
     2941 aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt
     3001 gattctccgg acgacgaaaa agccttttac tggctgaagc tggccgccga gcaaggtcat
     3061 tgtgaagcac aatattcctt aggacaaaag tatactgagg ataaaagccg tcataaagat
     3121 aatgagcatg ccatcttttg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc
     3181 aacgcccttg gctggatact ggatcgtgga gaagacccca attataaaga agcggttgtc
     3241 tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct tgggtggatg
     3301 tacagaaatg gcaacggagt cgcaaaagac tatgcgctgg catttttctg gtacaaacaa
     3361 gctgcattac aaggccatag tgacgcgcaa aacaatctgg ccgatcttta tgaagacgga
     3421 aaaggcgttg ctcaaaacga gacactcgcc gcattctggt atttgaaaag cgcacagcag
     3481 ggtaatcggc atgctcagtt tcaaattgca tgggattata acgctggcga aggggtggac
     3541 caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcaggg gagcgtcggc
     3601 gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa agattatcag
     3661 gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc ctggtataac
     3721 ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca ggctctcgac
     3781 ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat tcgtgagact
     3841 gaagatttac tgtagagata aaaacaaggg ccggggaatt tcattcggcc catattctct
     3901 ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga actggtcatg
     3961 accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat ggaagtgaca
     4021 tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa ccccatgatg
     4081 attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga tatctgccag
     4141 ctcctgccat aagctttctt taatcacccg cataaagacg ctgtcagatt cttctttcag
     4201 gctctcttca tagctcatgg cgaactcttc caggtcacgt cttacagctc gcgtcagctc
     4261 atcgacctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg cctgttccac
     4321 cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ctcagtgacg cgaccagttc
     4381 acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa ctgactataa
     4441 taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt aaattaatgt
     4501 atgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt gacgcggacg
     4561 tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt agccgtattg
     4621 aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat agaatccaaa
     4681 gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga gagcaaagag
     4741 aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat gggccacgta
     4801 cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg caaaaacgtc
     4861 ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc ggtgaaaaac
     4921 aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa ccagctcaaa
     4981 atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc ggaatactac
     5041 cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata taagaagact
     5101 tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca agttatcgac
     5161 ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca gtggtttatc
     5221 aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga tcactggcca
     5281 gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt ggagatcacc
     5341 ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc ggacaccttt
     5401 atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa agcggcggaa
     5461 aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt tgccgaagct
     5521 gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt tcacccatta
     5581 acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta cggcacgggc
     5641 gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc taaatacggc
     5701 ctgaacatca aaccagttat cctggctgct gacggctctg agccagatct ttctcagcaa
     5761 gccctgactg aaaaaggcgt gctgttcaac tctggcgaat tcaacggtct tgaccatgaa
     5821 gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga gcgtaaagtg
     5881 aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc gccgattccg
     5941 atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct gccggtgatc
     6001 ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga tccggagtgg
     6061 gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt cgacaccttt
     6121 atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga aggtatgctg
     6181 gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg tatcgaacac
     6241 gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga tgcaggcatg
     6301 gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct ggcagatgcc
     6361 ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga tgctatcgtt
     6421 gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca tgaactggtt
     6481 tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc gcaggtgatg
     6541 gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc tccggctgat
     6601 atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct gaaacgtgtc
     6661 tggaaactgg tttacgagca cacagcaaaa ggtgatgttg cggcactgaa cgttgatgcg
     6721 ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc taaagtgacc
     6781 gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat ggagctgatg
     6841 aacaaactgg cgaaagcacc aaccgatggc gagcaggatc gcgctctgat gcaggaagcg
     6901 ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt cacgctgtgg
     6961 caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc tgacgaaaaa
     7021 gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt ccgtgccaaa
     7081 atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg ccaggaacat
     7141 ctggtagcaa aatatcttga tggcgttacc gtgcgtaaag tgatttacgt accaggtaaa
     7201 ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg gcaacattgt
     7261 tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt gataccacgc
     7321 aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac gggccattaa
     7381 gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat aaagaaacca
     7441 cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa gataccgcat
     7501 cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg aatgcgaccg
     7561 tgttgatccc cggccgtgat atctacccga ttagtgccaa agtcttccgt tcgttctttg
     7621 ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta aaagagatgt
     7681 acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct gcggatattc
     7741 gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct gcacgcgtct
     7801 ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg cgcagctcaa
     7861 tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat tgcaggaaag
     7921 ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc acactttttc
     7981 cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta tgagtctgtt
     8041 tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg cggcgatcaa
     8101 tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga tcgtccgcgg
     8161 taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg cgaatcgcag
     8221 cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg ttgctgcgcg
     8281 cgcaaaacag ctcaacttag aactggatga cgctgcaaat caggtgctct gctactgtta
     8341 tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc tctggccaga
     8401 cggcaaattg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc atttcacccc
     8461 ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc atattcttca
     8521 gcaactgcgt ctggaaggca gcgaaccggt tattttgttg cgcacattac aacgtgaact
     8581 gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg cgttgtttga
     8641 taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa atcgcttaag
     8701 tcagacgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca ccctcaaaca
     8761 agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt tgtgccataa
     8821 acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct ctgtttggcg
     8881 gcacctttga tccggtgcac tatggtcatc ttaaacccgt ggaaacgctg gcgaatttga
     8941 ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt ccccagccgg
     9001 aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac aagccattat
     9061 ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg caaacactga
     9121 aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt ggtcaggatt
     9181 cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat gcacatttga
     9241 tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac cagcaatggc
     9301 tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc ggtaaaattt
     9361 atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa cgtttgcaaa
     9421 acggtgaatc gtgtgaggat ttattgccgg aaccggtact gacttacatt aaccaacaag
     9481 gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt taattcgtca
     9541 tggtgaaacg caagcgaata tcgatggtct atacagcggt catgcgccca cccccctgac
     9601 cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg tttcctttga
     9661 tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg ttctcagtga
     9721 ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg gcgactggga
     9781 gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg cgtggtgcaa
     9841 tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt cgcaacgtgt
     9901 ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt tagtcgtcag
     9961 ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg ccgaggctat
    10021 gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc aaaaattcgc
    10081 gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac ttttctgttt
    10141 ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat tctccgcagc
    10201 cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg gcgatgcaat
    10261 ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga caattgtccc
    10321 gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga ttcacccagg
    10381 gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt gatgacctca
    10441 aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc gactgcatga
    10501 tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac gttgtgcagg
    10561 agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc gccgactgga
    10621 ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc cgtcgcctgt
    10681 atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc gtgggaacga
    10741 aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt ccgaaagata
    10801 tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg gacatcaagc
    10861 gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac cgcattgtca
    10921 ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag ctggaacgct
    10981 ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg ttgtcgcctg
    11041 cctgtaaagc ggcggcggag cagagctggt cgctgtcgac gcttaccctc ccccatccgc
    11101 tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc accaaccatc
    11161 cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg aaactacaga
    11221 actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg ctggtcgcct
    11281 ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat ctgcaaattg
    11341 ttcgctttac cgattaccag acccgctcta atgaaaaccg cattaagctg gtgcctatcg
    11401 cgcccagccg cggcattatc tacgaccgta acggtatccc tctggcactc aaccgcacta
    11461 tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg ctggacgctt
    11521 tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa gagcgcgcac
    11581 gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta caagtagctc
    11641 gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat aaacgtcgtt
    11701 actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa atcaacgata
    11761 aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca acgcatgata
    11821 tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag accggttatg
    11881 aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa gtaccaccgc
    11941 aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat attgaaacgc
    12001 tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt ggggtgctgg
    12061 cactggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc tccagcaaag
    12121 attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc acacaggggg
    12181 tttatcctcc tgcgtctaca gttaaaccct atgtggcggt ttcggcatta agcgccgggg
    12241 tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca gggtcggaaa
    12301 aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca agatcgctgg
    12361 aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc gatcgcctct
    12421 ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg gcggaagaac
    12481 gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa ccgtggtatc
    12541 agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc ccaatccaga
    12601 tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg catttgctga
    12661 tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa ccgcccgtcg
    12721 gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt gttgctaacc
    12781 gccctaacgg tacggcacat aaatactttg ccagcgcacc gtacaaaatt gcggcgaaat
    12841 ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg cacaaaattg
    12901 ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac aatccgcaag
    12961 tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt acgctgatgc
    13021 gccagatcct cgaccacatt atgctgggtg ataacaatac cgatctgcct gcggaaaatc
    13081 ctgcggttgc cgcagcggag gaccattaat catgacggat aatccgaata aaaaaacatt
    13141 ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat tgctggttta
    13201 cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg agcgtaaaat
    13261 aggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc ctccacgcgt
    13321 ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg tggcggtaga
    13381 tgctttcggt gccatctcta aaggcgctca acgctggctg gacctcggta ttgttcgttt
    13441 tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct ttatcaaccg
    13501 cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga tatttatgcc
    13561 cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg cgctttccgg
    13621 tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg cagtagtgct
    13681 ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc agcgccagcg
    13741 cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc acattattca
    13801 gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc atggcactca
    13861 gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg tactggcgga
    13921 agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc tgatcatgcg
    13981 cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg ctggtggctt
    14041 aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg gtattctgcc
    14101 ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa ttgtgctgat
    14161 ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt cgaaaagcgt
    14221 gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca ggaatgctcg
    14281 cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag cctgcggtat
    14341 gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca ctgaacgcga
    14401 cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag gatccgtctc
    14461 gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt aacctgacgg
    14521 cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg cttccgatcc
    14581 ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg cgcattaatg
    14641 atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca gctgaccgtc
    14701 ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc caggatggtt
    14761 cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac gccctgcctg
    14821 cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg cagggtgaca
    14881 ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gatcggcgcg ccggtaacca
    14941 gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa ggcagcgaac
    15001 cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca acctcgcctg
    15061 caatggtgac accgcaagcc gcctcgcaaa gcgccagtgg caactttatg gtgcaagtcg
    15121 gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga cagaagttcg
    15181 gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt ggcccattcg
    15241 ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc caattacagt
    15301 catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat ttctgtaaat
    15361 gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt gctatagtag
    15421 ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    15481 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    15541 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    15601 agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag aacgcagatg
    15661 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    15721 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    15781 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    15841 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    15901 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    15961 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    16021 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    16081 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    16141 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    16201 acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc ttgatctctg
    16261 cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    16321 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    16381 aaccggtttg gtttggtgat tctgatcgcg cttcgttagg tgttgataaa gacgtgtacc
    16441 tgaccattcc acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    16501 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    16561 gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    16621 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    16681 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    16741 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    16801 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    16861 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    16921 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    16981 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    17041 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    17101 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    17161 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg
    17221 aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc
    17281 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    17341 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga caacaggtga
    17401 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    17461 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    17521 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgaattcgac
    17581 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    17641 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    17701 aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca
    17761 atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg
    17821 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tattttcttt
    17881 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    17941 ttgaaatagt tgattatggt gagcaaa
//
LOCUS       DAZFVO020000102        17958 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFVO020000102 DAZFVO020000000
VERSION     DAZFVO020000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.103"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            23..586
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS16210"
                     /old_locus_tag="pgaptmp_003242"
     CDS             23..586
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS16210"
                     /old_locus_tag="pgaptmp_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4196705.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIEILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            947..1657
                     /locus_tag="ACHKNB_RS16215"
                     /old_locus_tag="pgaptmp_003243"
     CDS             947..1657
                     /locus_tag="ACHKNB_RS16215"
                     /old_locus_tag="pgaptmp_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4196706.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1699..4350
                     /locus_tag="ACHKNB_RS16220"
                     /old_locus_tag="pgaptmp_003244"
     CDS             1699..4350
                     /locus_tag="ACHKNB_RS16220"
                     /old_locus_tag="pgaptmp_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4196707.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            4364..4894
                     /gene="ydeS"
                     /locus_tag="ACHKNB_RS16225"
                     /old_locus_tag="pgaptmp_003245"
     CDS             4364..4894
                     /gene="ydeS"
                     /locus_tag="ACHKNB_RS16225"
                     /old_locus_tag="pgaptmp_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196708.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAEMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4907..5410
                     /gene="ydeR"
                     /locus_tag="ACHKNB_RS16230"
                     /old_locus_tag="pgaptmp_003246"
     CDS             4907..5410
                     /gene="ydeR"
                     /locus_tag="ACHKNB_RS16230"
                     /old_locus_tag="pgaptmp_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196709.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5469..6383
                     /gene="ydeQ"
                     /locus_tag="ACHKNB_RS16235"
                     /old_locus_tag="pgaptmp_003247"
     CDS             5469..6383
                     /gene="ydeQ"
                     /locus_tag="ACHKNB_RS16235"
                     /old_locus_tag="pgaptmp_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196710.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            6717..6842
                     /locus_tag="ACHKNB_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /pseudo
     CDS             6717..6842
                     /locus_tag="ACHKNB_RS16240"
                     /old_locus_tag="pgaptmp_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6846..7424
                     /gene="ydeO"
                     /locus_tag="ACHKNB_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /pseudo
     CDS             6846..7424
                     /gene="ydeO"
                     /locus_tag="ACHKNB_RS16245"
                     /old_locus_tag="pgaptmp_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
     gene            7826..9508
                     /gene="ydeN"
                     /locus_tag="ACHKNB_RS16250"
                     /old_locus_tag="pgaptmp_003250"
     CDS             7826..9508
                     /gene="ydeN"
                     /locus_tag="ACHKNB_RS16250"
                     /old_locus_tag="pgaptmp_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4196711.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            9560..10717
                     /gene="ydeM"
                     /locus_tag="ACHKNB_RS16255"
                     /old_locus_tag="pgaptmp_003251"
     CDS             9560..10717
                     /gene="ydeM"
                     /locus_tag="ACHKNB_RS16255"
                     /old_locus_tag="pgaptmp_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HGU4196712.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQCYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(10683..10805)
                     /locus_tag="ACHKNB_RS16260"
                     /old_locus_tag="pgaptmp_003252"
     CDS             complement(10683..10805)
                     /locus_tag="ACHKNB_RS16260"
                     /old_locus_tag="pgaptmp_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196713.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            11008..12693
                     /gene="yddA"
                     /locus_tag="ACHKNB_RS16265"
                     /old_locus_tag="pgaptmp_003253"
     CDS             11008..12693
                     /gene="yddA"
                     /locus_tag="ACHKNB_RS16265"
                     /old_locus_tag="pgaptmp_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HGU4196714.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     IAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEILHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            12731..15103
                     /gene="yddB"
                     /locus_tag="ACHKNB_RS16270"
                     /old_locus_tag="pgaptmp_003254"
     CDS             12731..15103
                     /gene="yddB"
                     /locus_tag="ACHKNB_RS16270"
                     /old_locus_tag="pgaptmp_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HGU4196715.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDTWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            15160..17943
                     /gene="pqqL"
                     /locus_tag="ACHKNB_RS16275"
                     /old_locus_tag="pgaptmp_003255"
     CDS             15160..17943
                     /gene="pqqL"
                     /locus_tag="ACHKNB_RS16275"
                     /old_locus_tag="pgaptmp_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGU4196716.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         5247 a         3883 c         3932 g         4896 t
ORIGIN      
        1 taaatataaa ttttggagaa ttatgaaact caaacatgtt ggtatgattg tcgtttctgt
       61 gttggcgatg tcgtctgctg cggtaagcgc agccgagggt gatgaatcag taacgaccac
      121 tgttaatggc ggtgttattc attttaaagg tgaagtggta aatgccgctt gtgcaattga
      181 ttcagaatca atgaaccaaa cggttgagct gggtcaggtt cgttcttctc gcctggctaa
      241 agcgggtgac ctcagctccg ccgttggctt caatatcaag ctgaatgatt gtgataccaa
      301 tgtttccagt aatgcagctg ttgcattcct gggtactact gtcaccagta atgacgatac
      361 gttagcgctg cagagttcag cggcaggctc tgcccaaaat gtcggtattg aaattttgga
      421 ccgtacgggt gaggtattaa tacttgatgg ggccactttt agtgctaaaa ccgacttgat
      481 tgatggcacg aatatactgc cattccaggc tcgttatatt gctctcgggc agtccgtagc
      541 tggtactgca aacgctgatg cgaccttcaa agttcaatat ctataattca atcttaaggt
      601 ggctatacgt catctgaaat tgaaacaggg tgtgtgtggc atggctacgc acacccgagc
      661 aatttaccaa tattcatctc atttttaatg agaaatggtt tgtatttgga gcgctatgaa
      721 aaatcacata atatctaaac tatcaataaa caacagtagt taaacttact gttatttaaa
      781 atatttactt tgtagcaagg ttttcctatt aaaagagaac caacatttat tacaaacgca
      841 ttaagtctgg aaacccggcg actcgccaat gtattagcaa cattaatcga ttgtctgatg
      901 taacggtttt cccgcttatc ctcttaacac atggagaatg catcagatgc aaactacgcg
      961 taccccctac tctatttcct ttatggcgac agtgttactt ttattgctct ttgcttgcca
     1021 tagcaccgtt gccaacgcag ctgtcgcact gggtgcgacg cgggtaattt atccggcaaa
     1081 ccagaaacag gttttattgc ctgtcacgaa taatgatcct gccagtgtct atctcattca
     1141 gtcatggata gaaaatgcag gtgaccaaaa agacacccag tttgtcatta ccccaccgct
     1201 tttttccatg caaggtaaaa aagagaatac gctgcggata atcaacgcta ctaatcatca
     1261 gttaccaggc gaccgtgaaa gcctgttctg ggtgaatgtt aaagctattc ctgctatgga
     1321 aaaagaccag aagaatgaga atacattaca gctggcaatt attagccgca taaaaatgtt
     1381 ttatcgcccc acgaacctgg caatggcacc tgaagaagcg cctgcaatgc ttcggtttcg
     1441 tcgcagcggt agcaaactca cgcttattaa tccaacgccc tatttcatta ccgtaacaaa
     1501 tatgaaagca ggaaatagca atctcccaaa caccatggtg ccgccaaaag gtgaagtatc
     1561 ggtggatatc acgcatgctg caaccgggga tatcagtttt cagacgatca atgattacgg
     1621 tgcattaact ccgcgtatta aggcaaccat gcagtaagcc agtgacctaa ttaataataa
     1681 actcaatcgg aattaataat gacagccttt cgtgccgcat ttaaggcata tcgtatgcat
     1741 caggtgttaa taatgcctcg ctttgcacgg cttaccattg cccttggctt agcaactgcg
     1801 gtatttcccg ttgacgcaga gtattatttt aatccacggt ttttaagcaa tgatcttgct
     1861 gaatctgtcg atttatccgc ctttactaag ggacgcgagg caccacctgg tacatatcgg
     1921 gtcgacattt atttgaacga cgaatttatg actagtcggg atattacttt tattgcagat
     1981 gataataacg cagaactcat cccatgcctg agtacagatc ttcttgtcag tcttggtata
     2041 aaaaaaatag cgttattaga caataaagaa cattcggcag aaaaacatgt gccggacaac
     2101 agcgcatgca caccacttca ggatcgtttg gccgacgcat caactgagtt tgatgttggt
     2161 cagcaacatt tgtcactctc cgttccgcaa atttatgttg gcagaatggc tcgcggctat
     2221 gtttctcctg atctgtggga agaaggaata aatgctgggc ttctaaacta tagttttaat
     2281 ggtaattcta ttaataatcg tagtaaccat aatgcaggaa aatccaacta tgcatatttg
     2341 aatttacaga gtggcatcaa cattggtagt tggcgactgc gcgataactc aacgtggagt
     2401 tattacagtg ggagtagcaa ttcatctgac agcaataaat ggcagcatat caatacgtcg
     2461 gctgaacgtg acattattcc cttacgctca cggttaacgg taggtgatag ttataccgat
     2521 ggcgatattt ttgatagtgt gaactttcgt ggcctcaaaa taaattcaac agaagcgatg
     2581 ttgcccgata gccaacatgg tttcgctccg gtgattcatg gtattgcccg tggaaccgca
     2641 caagtgagtg taaaacagaa cggatacgat gtttatcaga ctactgttcc acccggccct
     2701 tttactattg atgatatcaa ctctgcggcc aatggtggtg atctgcaagt aaccataaaa
     2761 gaggcagacg gcagtattca gacattatat gttccttatt cgtctgttcc ggttctccaa
     2821 cgtgctggat atacgcgtta tgcgcttgcc atgggggaat atcgtagtgg aaataacctg
     2881 caaagctctc ccaagttcat acaaggtagc ttgatgcatg gtctggaagg aaactggaca
     2941 ccttatggcg gaatgcaaat tgcagaagat tatcaggcct tcaaccttgg tattgggaaa
     3001 gatttaggac tttttggtgc cttttctttc gatatcacgc aggccaatac gacacttgca
     3061 gatggcaccc gtcacagtgg gcaatcggtt aaatccgtct acagcaaatc cttctaccag
     3121 acaggaacca atatccaggt cgcaggatat cgctattcta cgcaaggttt ttataactta
     3181 tccgacagtg cctacagtcg aatgagtggt tacaccgtca agcctcctac cggagacacc
     3241 aatgagcaga cacaatttat tgattatttt aatctgttct acagtaagcg tggtcaggaa
     3301 caaataagca tctctcagca gcttggaaat tacggtacga catttttcag tgccagtcgc
     3361 caaagttact ggaacacgtc acgcagcgac cagcaaatat catttggatt aaatgtgccg
     3421 tttggtgata ttacgacttc gctgaattac agctattcca ataatatatg gcaaaacgat
     3481 cgggatcatt tactcgcttt tacgcttaat gttcccttca gtcattggat gcgtacagac
     3541 agtcagtcgg catttcgtaa ttcaaacgcc agttacagta tgtcaaacga tttgaaaggc
     3601 ggcatgacca atctatcggg ggtttatggc actctgctgc cggataataa cctgaattat
     3661 agcgttcagg tcggtaacac ccacggaggt aatacatcgt ctggcaccag tggttacagt
     3721 tctcttaatt atcgtggagc ttatggtaat actaatgtcg gttacagtcg gagtggtgac
     3781 agcagccaga tttattacgg aatgagtggt gggattattg ctcatgctga tggcatcacc
     3841 tttggacagc cgctgggcga cacaatggtt ctggttaagg ctcctggtgc tgataatgtc
     3901 aaaatagaga accagaccgg aattcatacc gactggcgtg gctatgccat attaccattt
     3961 gcgacagaat atagagaaaa ccgtgttgct cttaacgcga attcccttgc agataatgtt
     4021 gaactggatg aaaccgtggt cactgtcatc ccaactcacg gtgctattgc cagagcaaca
     4081 tttaatgcac aaatcggcgg gaaagtatta atgacgttga agtacggtaa taagagcgtt
     4141 ccattcggtg caattgtcac acacggagag aataaaaatg gcagcattgt cgcagaaaac
     4201 ggtcaggttt atctgactgg acttccacag tcagggaaat tacaggtttc atggggcaaa
     4261 gataaaaact caaactgtat tgtcgagtac aagcttcctg aagtttctcc tggtaccttg
     4321 ctgaaccagc agacagcaat ctgtcgctaa ggaacaatag attatgaaat acaataacat
     4381 tattttcctc ggtttatgtc tggggttaac cacctattct gctttatccg cagatagcgt
     4441 tattaaaatt agcgggcgcg tcctcgatta tggctgcaca gtctcatcgg attcgcttaa
     4501 ttttaccgta gatctccaaa aaaacagtgc cagacaattt ccaacgaccg gtagcacaag
     4561 tccagccgtc ccttttcaga ttacgttaag tgaatgcagc aaagggacaa cgggggttcg
     4621 ggttgcattt aacggtattg aggatgcaga aaataatact ttgttgaaac tggatgaagg
     4681 aagcaatacg gcctccggtt tgggtataga aatattggac gcaaatatgc gtccggtgaa
     4741 actgaatgac cttcatgccg agatgcagtg gatcccactg gtaccagaac agaacaatat
     4801 tttgccttac tccgctcgtc tgaagtcaac tcagaagtcc gtcaatccgg gactggtgag
     4861 ggcttcggca acctttaccc ttgaatttca ataatcagag tcagatatga aaagacttca
     4921 caagaggttc ctgttagcta cgttttgcgc gttattcaca gcaactctcc aggccgccga
     4981 tgtcactatc actgttaatg gtcgggtagt cgctaaaccc tgcactattc aaaccaaaga
     5041 agctaacgtt aatctcgggg atctttatac gcgcaatctg caacaacctg gttctgcatc
     5101 tggctggcac aatattactt tgtcattaac cgattgtccg gttgaaacaa gtgcagtgac
     5161 ggcaatcgtg acaggttcaa ctgacaatac gggttattac aaaaatgaag gtactgccga
     5221 aaatattcag atagagctga gggatgacca ggatgctgcg ttaaaaaatg gcgatagcaa
     5281 aacggttatt gttgatgaga tcactcgtaa tgcacagttt ccacttaagg caagagctat
     5341 cacggtgaat ggaaacgcaa gccagggaac gatcgaggcg ctaatcaatg tgatctacac
     5401 ctggcaataa cgtcgattcg attttcttag ccttcttttt aaatcaattt ttaaccttag
     5461 gatatatcat gggtaaaaca atcagtataa aggtcctgtt cggtatttat cttctattaa
     5521 tggcaggcaa agtttttgcc ttcagttgta atgtcgatgg cgggtcaagt attggagcag
     5581 gtacaacctc tgtttatgtt aatctcgacc ctgtaataca gccgggccag aatctggttg
     5641 tagacttgtc tcagcatata agttgctgga atgattacgg cggctggtac gacactgatc
     5701 atataaacct ggtacaaggc tcagctttcg caggctccct acagtcatat aaaggatcgc
     5761 tgtactggaa taatgtgacc taccccttcc cgttgacgac aaataccaac gtattggata
     5821 ttggtgataa gacaccgatg ccattaccgt taaaactcta tattacccca gttggggcgg
     5881 ccggtggagt tgtcataaaa gcgggggaag tcattgcccg tattcatatg tataaaattg
     5941 caacattagg aagcggaaat ccgcgtaatt ttacgtggaa tattatttca aataacaatg
     6001 ttgttatgcc aacaggcggc tgcaccgttg atagtcgtaa tgtcaccgtg gatctcccgg
     6061 actttcctgg aagcgcagaa ataccccttg gcgtatattg ctcaagcgaa caaaagctct
     6121 cattttatct ttcgggtgcc accactgaca gtagccgaca agtgtttgct aacacagcac
     6181 ctgatgcaac aaaagcatcg ggagtgggtg tcactctaat gcgtaatgga aaaatactgg
     6241 ctacgggaga aaatgtctcc ctggggacag taaataaaag caaagtacct ctgggtttaa
     6301 gcgcaaccta tggacaaaca ggaaacaagg tttcagccgg aaccgtccag tcagtcattg
     6361 gtgtcacttt tatctatgaa taattgtgat tttgacctta gtctatgcgc tccattcacg
     6421 aaaattgcgc ggataattat tttgtgaagg ctattagcct acacctgtaa gaaaatccgc
     6481 gcacaattga agccagagtc ttatttttta tattgaaaaa tatgttaatt taaacactat
     6541 gagagatgat gcttaactta tcgtatttaa tctattgttt aacgataata gattaaaaat
     6601 agaaagagag tagtccccta ctcttatata tccatgttgg cgataatccc tttgtatgta
     6661 ctgtgcatca tcgctattac aaatcctaat aattcatttc cacacaggat aagtagatga
     6721 agaaaaaaat tgaatcctac cagggtgctg caggtggttg gggtgctgtc aaatccgtag
     6781 cgaatgcagt acgtaagcag atggatatac gccaggatgt tattgccatg tttgacatga
     6841 ataatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg aagtttttac
     6901 gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc aactcggaag
     6961 aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct atttttgccg
     7021 catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc ggaaaagtga
     7081 gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt tgtgactgcc
     7141 tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg acattctcac
     7201 agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta gaaggttcag
     7261 tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt tatgcgttcc
     7321 gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt caaagtcaca
     7381 cgggtatgaa tacgggcaac acgatgagtg ctttagctat ttgattattt gctaacgagt
     7441 agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc cccgcgttgt
     7501 ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt tatgcacttg
     7561 cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt aaaatgcaat
     7621 aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta atagaacaga
     7681 aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat ctgctatctc
     7741 tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta cttcggaatt
     7801 aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg tcgtaagtac
     7861 ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg cggcagatga
     7921 tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc cgactgaata
     7981 cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg gttatggaca
     8041 acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg aagttgtcga
     8101 tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa cgccgacgct
     8161 cctttcatta atggatgaag gcgtacggtt tactaacggc tatgtggcac acggtgtttc
     8221 cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg gtgtctattc
     8281 caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg aattattcca
     8341 gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa tcagtaatgt
     8401 gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca cattttctgc
     8461 ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc acgctgcagg
     8521 aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc ccgcaaaagg
     8581 ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg ccaaaacact
     8641 tgaccagcct tttatgcttt acctggctta taatgctccg cacctgccaa atgataatcc
     8701 tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag ataactacta
     8761 cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac tgaagaaaaa
     8821 cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg ttatcgatgg
     8881 tcctctgccg ctgaacgggg cgcaaaaagg ctataagagc cagacctatc ctggcggtac
     8941 tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt atgacaagct
     9001 gatttccgca atggatttct acccgacagc tcttgatgca gccgatatca gcattccaaa
     9061 agaccttaag ctggatggcg tttccttgct gccctggttg caagataaga aacaaggcga
     9121 gccacataaa aatctgacct ggataacctc ttattctcac tggtttgacg aggaaaatat
     9181 tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt acccgcataa
     9241 ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg attattcgct
     9301 tgtgtataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc tacagcaaaa
     9361 agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg tggtaagaga
     9421 gtttatcgac agcagccagc caccgcttag cgaagtaaat caggagaagt ttaacaatat
     9481 caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggaat tttccgccgc
     9541 cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc aatgtaatct
     9601 caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg aaaaatggat
     9661 ggatgacagc actctgaaag agttcatcaa acaatatatc gcagcgtctg gcaatcaggt
     9721 ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt tccgtaaagt
     9781 tattcactat caacaatgct atgcaggcca aaaacgtatt tttaatgcat tacaaacgaa
     9841 tggcatttta ttgaataatg aatggtgtgc ttttctcaaa gaacatgagt ttctggttgg
     9901 tatctcgatc gatggccccc aggagctaca tgaccgttac agacgcagta attcaggtaa
     9961 cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc aagttgagtt
    10021 taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg tttatcattt
    10081 tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctcgaaa ccgggacgcc
    10141 gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt tttctgtgcc
    10201 tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta aaaacgatgt
    10261 gggtgaaatt ttcatccgtc agtttgaaag ctttgtcagc cgttttttag ggaatgggca
    10321 taccagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa gtaatggaga
    10381 tatttatgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca ttaataaatc
    10441 tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga tttcagcgaa
    10501 atgtctgaaa tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac
    10561 taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac
    10621 catggtacct tatatgaacg ccatggtgga gttagctaag aacagagtac cgctttacca
    10681 cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt
    10741 cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc
    10801 gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc
    10861 aatacccttg attatcaata gcataaccat aaccactgaa aatgaaatat tcctttatat
    10921 ttcatattgt taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc
    10981 attggatggc acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg
    11041 atagcaaagt atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca
    11101 gtgttgttaa tcattattat cctcgccatg atccttggcg tggttaaaat tcaggtctgg
    11161 ttaaacgact ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt
    11221 tggcagcttg tcctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac
    11281 aaaacatggt taattaaact actgactatt cgctggcgcg aatggttaac agattactac
    11341 cttaatcggt ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa
    11401 aatacagata accctgacca acgtatcgct gaagacattc tcttactgat cagcaaaacg
    11461 ctcagccttt catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt
    11521 ctttggcaaa gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag
    11581 gggtatatgg tctataccgt tgtgctcatt gtgatcggtg gaactttatt tacccataag
    11641 gttggtaaac gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga
    11701 actaatcttg tgcagcataa taaacaggca gaactgattg cgctaagcaa tgctgagtct
    11761 ctacaacgcc aggagttgag cgacaatttt cacaccatta aagagaactg gcatcgttta
    11821 atgaatcgtc agcggtggct tgattactgg cagaatattt attcgcgttc gcttagcgtt
    11881 cttccgtact ttctgttatt gccgcagttt attagcggac agatcaatct gggcggactt
    11941 atgaaatcgc gccaggcatt tatgctggta tcgaacaatt taagctggtt tatttataaa
    12001 tatgatgaaa ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc
    12061 accgaacagc gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctgac
    12121 gcgagtattc gtacccctga taataagatc atattagaga acctgaactt tcatgttccg
    12181 ccaggcaaat ggctattact gaaaggttac tctggtgcgg gaaaaaccac actgcttaaa
    12241 acattatccc actgctggcc gtggtttaaa ggtgatattt cttctcctgc cgacagttgg
    12301 tatgtgtcac aaacaccgtt aatcaaaacc ggcttactga aagagattat ttgtaaagca
    12361 cttcctctgc ccgtagacga taaatcgttg agcgaaatac tgcatcaggt tggtctgggg
    12421 aaattggctg cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa
    12481 aaacaacgta tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac
    12541 gaaactacct ctcatcttga ggaacaagag gctatccgct tactgcgttt agtgcgtgaa
    12601 aaactaccca caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggcc
    12661 gatgatattt gtgacattag cgcggtttta taaaaacaat aaaagccctg ctgtacggag
    12721 atataaataa atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc
    12781 gcaggccgtc gatgacaaaa acatgtacat gcatgttttt gaagagatga cggtctatgc
    12841 tcctgtccct gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt
    12901 accgaccggg aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga
    12961 ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc ccggagaaaa tctctattca
    13021 cggtgcgtcg ccctaccaga atgcctattt gattgatggt attagtgcca ctaataacct
    13081 gaacccagcg aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg
    13141 gtattatctt gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt
    13201 tgaattcggt cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga
    13261 tgatagcagc gtgaaactgg gttatcgcac tacgcgttcg gactggttaa catcgcatat
    13321 cgatgagaat aacaagagcg catttaatca aggttcttca ggaagtactt attactctcc
    13381 agattttaaa aagaactttt ataccttgtc gtttaatcag gaactcgctg ataactttgg
    13441 cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa
    13501 tgacggcatt gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa
    13561 atttacctgg tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc
    13621 cagccgtgat tataatacca gcaccttccc gcagtctgat cgcgaaatgg gtaataaatc
    13681 ctatggtctg gcatgggata tggatacgca gctcgcatgg gccaaacttc gtaccaccgt
    13741 tggttgggat catattagtg attatacccg tcacgatcat gacacctggt acaccgaact
    13801 ttcatgtaca tatggtgata ttacagggcg ttgcacccgt ggcggattag gacacatttc
    13861 ccaggctgta gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg
    13921 taatgtttcg catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga
    13981 acgccataac cagtctgaat cctatgtgat taatgctgcc ggaaagaaaa ctaaccatac
    14041 catttaccat aaaggtaaag gcagcctggg aattgacaac tacacactgt atatggcgga
    14101 tcgcattagc tggcgtaatg tgtcattaat gcccggcgtg cggtatgact atgacaacta
    14161 tctgtcaaac cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca
    14221 aacctcaatg attaccgcag gttataaccg ttactatggt gggaatattc ttgatatggg
    14281 attacgtgat atccgcaata gctggacgga atcggtatca ggcaataaaa ccctgacgcg
    14341 ttatcaggat ttgaaaacgc cttataacga tgaactggca atgggattgc agcagaaaat
    14401 cggtaagaac gttattgcgc gtgcaaacta tgtttaccgt gaagcgcatg atcaaatcag
    14461 caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg
    14521 caaaaccaaa acgcattcgt tcaacctcag ttttgagctg gccgaacccc tgcatatccg
    14581 ccaggtagat attaacccac aaattgtctt tagctatatc aagagcaagg gcaacttgtc
    14641 gttaaacaat ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa
    14701 tcttgtctct tacgatagcg ttccagtggc agattttaat aacccattaa agatctcctt
    14761 aaacatggat ttcacgcatc aaccgagcgg gttggtgtgg gcgaatacgc tggcctggca
    14821 agaagcgcgt aaagctcgca ttatcctggg taagacaaat gcgcaataca tcagcgaata
    14881 ttcagattac aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg
    14941 cttgtcctgg acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct
    15001 caatgtattg gatagcaaaa ccgctgttga tacaacgaat accggtgtgg cgacctacgc
    15061 cagtggccgt actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt
    15121 aaccgggtaa attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta
    15181 ctgacgttag tggcaactct gttgctcccc gggcggctga ttgccgccgc cttaccgcag
    15241 gatgaaaagt taattaccgg gcaactgggc aatggcttgc gatatatgat ttatccgcat
    15301 gctcatccaa aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa
    15361 gaagacaatg agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcacaaaa
    15421 acatggccgg gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtcgc
    15481 gatgttaatg cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg
    15541 cagaaacaaa atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc
    15601 tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta ctgaggaatg gcgtgcccat
    15661 caggatgcga aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt
    15721 aatttagacc gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa
    15781 ttgcgccaat tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc
    15841 gatatcgaca gtaaagaagc gctggcgctg ataaaggata atttaagtaa gcttccggct
    15901 aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat
    15961 atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg
    16021 gtacaagtga acgatgagca aagctttatc gaacaagctg aatggagcat gttagttcag
    16081 ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc
    16141 ggcactgcgc gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat
    16201 gcacgagacg ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt
    16261 gatcagcatg gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg
    16321 ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg
    16381 gcatccagct cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg
    16441 aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga
    16501 aagaaccagg acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca
    16561 ttgtctcctg cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct
    16621 tacatcttcc caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt
    16681 agcagcaaag aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccagg
    16741 gttattctgg caaaatccgc gggtgaagag caaaagctac aaattactgc cgtatcgaat
    16801 aaaggcgatt taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca
    16861 gtaagcggaa gcggcgttgg tgagctctcc tcttccagcc tgaaacgctg gagtgcggaa
    16921 aattcggtaa ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg
    16981 cggactaata accctgaacc tggttttcag ttgattaacc agcgaatcac ccacagcacg
    17041 attaacgaca atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc
    17101 gaccagcgtc cagcggagaa attcgcccag cagatgtatg agacgcgcta tgctgatgac
    17161 cgcacgaaat tactgcaaga aaatcagatt gtacagttta ctgccgcaga tgcgctggct
    17221 gccgatcgcc agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc
    17281 tcagaagaca aactcgtggc gttaattacg cgttacttag gatcaatcaa acactctgat
    17341 tcgccattag ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta
    17401 aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca
    17461 cctgttaatc tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat
    17521 ctacgtgtta atattcgtga acaggcatct ggagcataca gcgtttcttc tcgcctctcg
    17581 gttgatcctc aggccaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga
    17641 catgatgaac tgttaacgtt agcgaatgaa gtgatggtta agcgtctggc taaagggatc
    17701 agtgagcaag aactgaatga ataccagcaa aacgttcagc gcagcctcga tatccaacag
    17761 cgtagcgttc aacaattagc gaacactatt gtaaatagtc ttattcaata tgacgatcct
    17821 gcagcatgga ctgagcagga gcaattgttg aaacaaatga cggtagagaa tgttaacact
    17881 gccgttaaac aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa
    17941 taataacagc cccgtcaa
//
LOCUS       DAZFVO020000103        17931 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFVO020000103 DAZFVO020000000
VERSION     DAZFVO020000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.104"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            63..1202
                     /gene="arnB"
                     /locus_tag="ACHKNB_RS16280"
                     /old_locus_tag="pgaptmp_003256"
     CDS             63..1202
                     /gene="arnB"
                     /locus_tag="ACHKNB_RS16280"
                     /old_locus_tag="pgaptmp_003256"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HGU4196717.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1206..2174
                     /gene="arnC"
                     /locus_tag="ACHKNB_RS16285"
                     /old_locus_tag="pgaptmp_003257"
     CDS             1206..2174
                     /gene="arnC"
                     /locus_tag="ACHKNB_RS16285"
                     /old_locus_tag="pgaptmp_003257"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HGU4196718.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2174..4156
                     /gene="arnA"
                     /locus_tag="ACHKNB_RS16290"
                     /old_locus_tag="pgaptmp_003258"
     CDS             2174..4156
                     /gene="arnA"
                     /locus_tag="ACHKNB_RS16290"
                     /old_locus_tag="pgaptmp_003258"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HGU4196719.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4153..5043
                     /gene="arnD"
                     /locus_tag="ACHKNB_RS16295"
                     /old_locus_tag="pgaptmp_003259"
     CDS             4153..5043
                     /gene="arnD"
                     /locus_tag="ACHKNB_RS16295"
                     /old_locus_tag="pgaptmp_003259"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HGU4196720.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5043..6695
                     /gene="arnT"
                     /locus_tag="ACHKNB_RS16300"
                     /old_locus_tag="pgaptmp_003260"
     CDS             5043..6695
                     /gene="arnT"
                     /locus_tag="ACHKNB_RS16300"
                     /old_locus_tag="pgaptmp_003260"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HGU4196721.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6692..7027
                     /gene="arnE"
                     /locus_tag="ACHKNB_RS16305"
                     /old_locus_tag="pgaptmp_003261"
     CDS             6692..7027
                     /gene="arnE"
                     /locus_tag="ACHKNB_RS16305"
                     /old_locus_tag="pgaptmp_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HGU4196722.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7027..7413
                     /gene="arnF"
                     /locus_tag="ACHKNB_RS16310"
                     /old_locus_tag="pgaptmp_003262"
     CDS             7027..7413
                     /gene="arnF"
                     /locus_tag="ACHKNB_RS16310"
                     /old_locus_tag="pgaptmp_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HGU4196723.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7407..7673)
                     /gene="pmrD"
                     /locus_tag="ACHKNB_RS16315"
                     /old_locus_tag="pgaptmp_003263"
     CDS             complement(7407..7673)
                     /gene="pmrD"
                     /locus_tag="ACHKNB_RS16315"
                     /old_locus_tag="pgaptmp_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HGU4196724.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7783..9138)
                     /gene="menE"
                     /locus_tag="ACHKNB_RS16320"
                     /old_locus_tag="pgaptmp_003264"
     CDS             complement(7783..9138)
                     /gene="menE"
                     /locus_tag="ACHKNB_RS16320"
                     /old_locus_tag="pgaptmp_003264"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HGU4196725.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9135..10097)
                     /gene="menC"
                     /locus_tag="ACHKNB_RS16325"
                     /old_locus_tag="pgaptmp_003265"
     CDS             complement(9135..10097)
                     /gene="menC"
                     /locus_tag="ACHKNB_RS16325"
                     /old_locus_tag="pgaptmp_003265"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HGU4196726.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10097..10954)
                     /gene="menB"
                     /locus_tag="ACHKNB_RS16330"
                     /old_locus_tag="pgaptmp_003266"
     CDS             complement(10097..10954)
                     /gene="menB"
                     /locus_tag="ACHKNB_RS16330"
                     /old_locus_tag="pgaptmp_003266"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HGU4196727.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10969..11727)
                     /gene="menH"
                     /locus_tag="ACHKNB_RS16335"
                     /old_locus_tag="pgaptmp_003267"
     CDS             complement(10969..11727)
                     /gene="menH"
                     /locus_tag="ACHKNB_RS16335"
                     /old_locus_tag="pgaptmp_003267"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HGU4196728.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11724..13394)
                     /gene="menD"
                     /locus_tag="ACHKNB_RS16340"
                     /old_locus_tag="pgaptmp_003268"
     CDS             complement(11724..13394)
                     /gene="menD"
                     /locus_tag="ACHKNB_RS16340"
                     /old_locus_tag="pgaptmp_003268"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HGU4196729.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13483..14778)
                     /gene="menF"
                     /locus_tag="ACHKNB_RS16345"
                     /old_locus_tag="pgaptmp_003269"
     CDS             complement(13483..14778)
                     /gene="menF"
                     /locus_tag="ACHKNB_RS16345"
                     /old_locus_tag="pgaptmp_003269"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HGU4196730.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     MREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14857..15162)
                     /gene="elaB"
                     /locus_tag="ACHKNB_RS16350"
                     /old_locus_tag="pgaptmp_003270"
     CDS             complement(14857..15162)
                     /gene="elaB"
                     /locus_tag="ACHKNB_RS16350"
                     /old_locus_tag="pgaptmp_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HGU4196731.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15217..15678)
                     /locus_tag="ACHKNB_RS16355"
                     /old_locus_tag="pgaptmp_003271"
     CDS             complement(15217..15678)
                     /locus_tag="ACHKNB_RS16355"
                     /old_locus_tag="pgaptmp_003271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4196732.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15743..16660
                     /gene="rbn"
                     /locus_tag="ACHKNB_RS16360"
                     /old_locus_tag="pgaptmp_003272"
     CDS             15743..16660
                     /gene="rbn"
                     /locus_tag="ACHKNB_RS16360"
                     /old_locus_tag="pgaptmp_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HGU4196733.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16848..17931
                     /gene="elaD"
                     /locus_tag="ACHKNB_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /pseudo
     CDS             16848..17931
                     /gene="elaD"
                     /locus_tag="ACHKNB_RS16365"
                     /old_locus_tag="pgaptmp_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
BASE COUNT         4278 a         4791 c         4498 g         4364 t
ORIGIN      
        1 atacacaaca ctctttatag caaatataag tggacaggta ttcaatggcg gaaggaaaag
       61 caatgtcaga atttttgcct ttttcgcgac cagcaatggg cgtggaggaa ctcgctgcag
      121 ttaaagaggt tctcgaatcc ggttggatca caaccggtcc gaagaatcag gcgcttgagc
      181 aagccttttg ccagttgacg ggaaatcagc atgccatcgc ggtcagttca gccaccgccg
      241 gaatgcatat cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg attacgcctt
      301 ccctgacctg ggtttcaacc ctcaatatga tttccttgtt gggtgcaacg ccggtaatgg
      361 tggatgtcga ccgcgatacg ctgatggtca cgcctgaagc gatcgagtca gccataacgc
      421 cacgcactaa agccatcatt ccggtgcatt atgccggtgc accagcagat attgacgcca
      481 ttcgcgccat tggcgaacgt tacggcatcg cagttatcga agatgctgcc catgccgtcg
      541 gtacgtatta caaagggcga catattggcg caaaaggtac cgctattttt tcatttcatg
      601 ccattaaaaa tattacctgt gcggaaggtg gcctgattgt aactgataat gaaaaccttg
      661 cccgccagct acggatgctg aaatttcacg gtctgggtgt cgatgcctat gacagacaaa
      721 cctggggccg tgcaccgcag gctgaagtct taacaccggg ctataagtac aatctgaccg
      781 atattaacgc cgcgattgcc ctgacacagt tagcaaaatt agagcacctc aacacccgtc
      841 ggcgcgaaat tgcccagcaa tatcagcaag cactggcagc tctccccttt cagccattaa
      901 gccttcccgc ctggccgcac gttcacgcct ggcatctgtt tattattcgt gtcgatgaac
      961 aacgttgtgg tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga ggcattggta
     1021 ccgggttaca tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt tttcccacgc
     1081 tgtcgttacc gaataccgaa tggaatagcg aacgcatctg ttctttgccg ctgttcccgg
     1141 atatgactac cgccgatgcc gaccgtgtca tcacagccct tcagcaactc gcaggacaat
     1201 aagccatgtt tgaaatccac cctgttaaga aagtctcggt ggttattccc gtttataacg
     1261 agcaggaaag cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa tcgttgggga
     1321 aagagtatga gatcctgctg attgatgacg gcagtagcga taattccgcg catatgctgg
     1381 tcgaagcctc acaagcggag aacagccata ttgtgtctat tttgcttaac cgcaattacg
     1441 ggcaacattc agcgattatg gcgggattca gtcacgttac cggcgactta attattaccc
     1501 ttgatgccga tctccagaat ccgccagaag aaatcccccg cctggtggca aaagccgatg
     1561 aaggttacga cgtggtaggg actgtacgcc agaaccgcca ggacagctgg tttcgtaaaa
     1621 cagcttcgaa gatgattaac cggcttattc agcgcaccac cggcaaagcg atgggtgact
     1681 acggttgtat gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg cactgccatg
     1741 aacgtagcac ctttatcccg attctggcga atatcttcgc ccgccgtgcc attgaaattc
     1801 cagtacatca ttccgagcgt gagtttggtg aatccaaata cagttttatg cgcctgatta
     1861 atttgatgta cgacctggtg acctgcctta ccacaacgcc gctacgtatg ctgagtctgc
     1921 tcggcagcat tattgcgatt ggaggtttta gcattgcggt gctgctggtg attttacgcc
     1981 tgaccttcgg accacaatgg gcggcagaag gcgtctttat gctatttgcc gtgctgttta
     2041 cttttattgg cgctcagttt atcggcatgg gattactcgg tgaatatatc ggcaggatct
     2101 acaccgatgt ccgcgcccgc ccccgctatt ttgttcagca agttatccgt ccatccagca
     2161 aggaaaatga ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat gcctcggtat
     2221 tgaagctctg ctggctgccg gttacgaaat tagcgccatt tttacccata ccgataatcc
     2281 cggtgaaaaa gccttttatg gttcggtggc tcgtctggca gcggaaagag gcattccggt
     2341 ttatgcgccg gatgacgtta atcatccgct gtgggtggaa cgcattgccc aactgtcgcc
     2401 agatgtgatt ttctcttttt attatcgcca tcttatttgc gacgaaattt tgcagctcgc
     2461 tcccgcaggt gcatttaatc tgcatggttc gctgttacca aaatatcgtg gtcgcgcgcc
     2521 gctgaactgg gtgctggtca acggtgaaac ggaaactggc gttacattgc accgaatggt
     2581 gaaacgtgcc gatgccgggg ccattgtggc gcaactgcgc attgccattg cgccagacga
     2641 tatcgctatt acgctgcatc ataaattgtg ccatgccgcg cgccagctac tggaacagac
     2701 attacccgcc attaaacacg gtaatattct ggaaatcgcc cagcgcgaaa acgaagccac
     2761 ctgttttggt cgcagaacgc cggatgacag tttccttgaa tggcataaac cggcatccgt
     2821 actgcacaac atggtacgtg ccgttgccga tccgtggccg ggtgccttca gctatgttgg
     2881 caatcagaaa ttcaccgtct ggtcgtcgcg tgttcatcct catgccagca aagcacagcc
     2941 ggggagcgtg atttctgttg cgccactgct gattgcctgt ggcgatggcg cgctggaaat
     3001 cgtcaccgga caggcgggcg acggcattac tatgcagggc tcgcaattag cgcagacgct
     3061 gggcctggtg caaggttcac gcttgaatag ccagcctgcc tgcaccgccc gacgccgtac
     3121 ccgggtactc atcctcgggg tgaatggctt tattggcaac catctgacag aacgcctgct
     3181 gcgcgaagat cattatgaag tttacggtct ggatattggc agtgatgcga taagccgttt
     3241 tctgaatcat ccgcattttc actttgttga aggcgatatc agtattcatt ccgaatggat
     3301 tgagtatcac gtcaaaaaat gtgatgtcgt cttgccgctg gtggcgatag ccacgccgat
     3361 tgaatatacc cgcaacccgc tgcgcgtatt tgaactcgat tttgaagaga atctgcgcat
     3421 tatccgctac tgcgtgaagt accgtaagcg aatcatcttc ccgtcaactt cagaagttta
     3481 tgggatgtgt agcgataaat acttcgatga ggaccattct aatttaatcg tcggcccggt
     3541 gaataaacca cgctggattt attcggtatc aaaacaatta cttgatcggg tgatctgggc
     3601 ctatggcgaa aaagagggtt tacagttcac cctcttccgc ccgtttaact ggatgggacc
     3661 gcgactggat aaccttaatg cggcacgaat cggcagctcc cgcgctatta cgcaactcat
     3721 tctcaatctg gtagaaggtt caccgattaa gctgattgat ggcggaaaac aaaaacgctg
     3781 ctttactgat attcgcgatg gtatcgaggc gttataccgc attatcgaaa atgcgggaaa
     3841 tcgctgcgac ggtgaaatta tcaacattgg caatcctgag aacgaagcga gcattgagga
     3901 actgggcgag atgctgctgg cgagcttcga aaaacatccg ctgcgccatc atttcccacc
     3961 gtttgcgggc tttcgcgttg tcgaaagtag cagctactac ggcaaaggat atcaggacgt
     4021 agagcatcgt aaaccgagca tccgcaatgc ccaccgctgc ctggactggg agccgaaaat
     4081 tgatatgcag gaaaccatcg acgaaacgct ggatttcttc ctgcgcaccg ttgatcttac
     4141 ggataaacca tcatgaccaa agtaggctta cgcattgatg tcgatacctt tcgtggcacc
     4201 cgtgaaggcg tgccgcgtct gctggaaatc ttgagtaagc ataatattca ggccagcatt
     4261 tttttcagcg tcggcccgga caatatgggc cgccatctct ggcgactggt gaagccacag
     4321 tttttgtgga agatgctgcg ctcaaatgcg gcatcgcttt atggctggga tattttactg
     4381 gcaggtacgg cctggccagg taaagagatt ggtcatgcca atgccgatat cattcgtgaa
     4441 gcggctaaac atcacgaagt cggcctgcac gcctgggatc accatgcctg gcaagcccgt
     4501 agcggtaact gggatcggca aacaatgatc gacgatattg cacgcggtct tcgcactctg
     4561 gaagagatta tcggtcaacc ggtaacctgt tctgccgctg cgggctggcg tgccgaccag
     4621 aaggtgatcg aagcaaaaga agcgttccat ttgcgctaca acagcgattg tcgtggggcc
     4681 atgccgttcc gtccattgct cgaatcagga aaccctggca ctgcgcaaat tccggtgacc
     4741 ttacccacct gggatgaagt gattggtcgg gatgtgaaag cagaagattt taacggttgg
     4801 ttactcaacc gcatcctgcg agataaaggc acgccggttt ataccattca tgcagaagtt
     4861 gaaggctgcg cttatcagca taattttgtg gatctcctca aacgcgcagc tcaggaaggc
     4921 gtgacatttt gccctttaag cgaactgtta tcagagacgt tgccgctcgg acaagttgtt
     4981 cgcggaaata ttgccggacg tgaaggctgg ctgggttgcc aacaaattgc gggtagtcgc
     5041 tgatgaaatc ggtacgttac cttatcggcc tcttcgcgtt tattgcctgc tattacctgt
     5101 taccgatcag cacgcgtctg ctctggcaac ccgatgaaac gcgttatgcg gaaatcagtc
     5161 gagaaatgct ggcatccggc gactggattg tgccccatct gttagggcta cgttatttcg
     5221 aaaaacccat tgccggatac tggattaaca gcattgggca atggctattt ggcgcgaata
     5281 actttggtgt gcgggcaggc gttatctttg cgaccctgtt aactgccgcg ctggtgacct
     5341 ggtttactct gcgcttatgg cgcgataaac gtctggctct actcgccaca gtaatttatc
     5401 tctcattgtt tattgtctat gccatcggca cttatgccgt gctcgatccg tttattgcat
     5461 tctggctggt ggcgggaatg tgcagcttct ggctggcaat gcaggcacag acgtggaaag
     5521 gcaaaagcgc aggattttta ctgctgggaa tcacctgcgg catgggggtg atgaccaaag
     5581 gttttctcgc ccttgccgtg ccggtattaa gcgtgctgcc atgggtagcg acgcaaaaac
     5641 gctggaaaga tctctttatt tacggctggc tggcggttat cagttgcgta ctgacggttc
     5701 tcccttgggg actggcgata gcgcagcggg agcctgactt ctggcactat tttttctggg
     5761 ttgagcatat tcaacgcttt gcactggatg atgcccaaca tagagctccg ttctggtact
     5821 acgtgccggt catcattgcc ggtagcctgc cgtggctggg attactcccc ggtgcactgt
     5881 acacaggctg gaaaaaccgc aagcattccg caaccgtcta tttgttgagc tggacgataa
     5941 tgccgctgct gtttttctcc gtcgctaaag gtaaattgcc cacctatatt ctttcctgct
     6001 ttgcatctct ggcaatgctg atggcgcatt acgctttgct ggcagcaaaa aataatcctc
     6061 tggcgctgcg gattaatggc tggattaaca tcgcttttgg cgtcactggc attattgcca
     6121 catttgtggt ctccccgtgg ggaccaatga acacgccggt gtggcaaacc ttcgagagct
     6181 ataaagtctt ttgtgcctgg tcgatttttt cgctatgggc atttttcggc tggtacacct
     6241 taacaaacgt cgaaaagacc tggccttttg ccgcgctttg cccgctgggg ctggcgttgc
     6301 tggtaggatt ttcaattcct gacagagtta tggaaggaaa acatccgcaa ttttttgtcg
     6361 agatgacaca agaatcactt cagccaagcc gctatattct tactgatagc gtcggtgttg
     6421 ccgcaggtct ggcatggagc ctgcaacgcg atgacatcat catgtatcgc cagacaggtg
     6481 agttgaaata cggccttaat tatccggatg cgaaagggag atttgtcagc ggtgatgagt
     6541 tcgcaaactg gcttaatcaa catcgtcagg aggggattat tactctcgtg ctttcggttg
     6601 accgcgatga agatatcaac agtctcgcca ttccgcccgc agatgccatc gatcgtcagg
     6661 agcgtctggt gctgattcag tatcgtccca aatgatctgg ctaacattag tctttgccag
     6721 cttgcttagc gttgccgggc agttgtgtca gaaacaggca acctgctttg tggcgataaa
     6781 caaacggcgc aaacatatcg tgctgtggct gggactggcg ctggcttgtc ttggtcttgc
     6841 catggtgctc tggctgctgg tcttgcagaa cgtaccggta ggcattgcct acccgatgtt
     6901 aagtctgaat tttgtctggg tgacgctggc tgcagtaaaa ctgtggcacg aaccggtatc
     6961 gccgcgtcac tggtgtgggg tggcgttcat tattggtggc attgtgatcc tcgggagtac
     7021 ggtgtaatgg gcctgatatg gggattattc agcgtcatta ttgcctcagt tgcgcaatta
     7081 agcctgggtt ttgcggcaag tcatctgccg ccgatgacgc acttatggga ttttatcgcg
     7141 gctctgttag cttttggcct ggatgcccgg atactgttac tcgggctgct gggttatctg
     7201 ctttccgttt tttgttggta taaaacgcta cataaacttg cgctaagcaa agcctatgcc
     7261 ttgctcagta tgagttatgt gctggtatgg attgcttcaa tggtacttcc cggctgggaa
     7321 gggacttttt cgctgaaagc actactggga gtagcctgta ttatgagcgg gttgatgctg
     7381 attttcctgc ccacgacaaa acaacgttac tgagttttcc ctgccgctgt acactgccgt
     7441 tcccactcat cggggctata actgcttgcc gagaggactt tcaccgtatg cagcttttca
     7501 cgattaatac aataaagcgc attctgcaaa ggcgagagta aatcccccac tttcacggcg
     7561 aagtcactct tcacttcggc gatcattttt atcgcgccgc cagcatcgca gagcataagt
     7621 acatgtctgt tatcttgttt attgcagcac gattttttga ccagccattc cattgcatta
     7681 tcctgtttgc taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac
     7741 ggcaatggaa tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac
     7801 ccactctttt agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa
     7861 agtcagccag cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag
     7921 atcaacggtt tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc
     7981 ggcaacggga acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac
     8041 ttcttccggc tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat
     8101 ggtcagcttg ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt
     8161 aaccagtgaa accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag
     8221 ccacacttca ttattaacga ttttcacttc ccgacccggc agcggcgaac caacgtctgc
     8281 caggccgtcg gcttctttcg cacacaccgt ggaggcaaac tcggtcagac catagccgca
     8341 aaagcaacga atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc
     8401 gccaagtaac accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg
     8461 tgttggcacc agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc
     8521 acgcaccgtc atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac
     8581 gtgaaacagc ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcaacgataa
     8641 caccccttcg gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaccc
     8701 ggtagaaccc gaggtcaacg tcattgagca cagacgcgtc ggctgccacg tagcggcatg
     8761 tgcgccttca acccgctgaa tgtgcagcga cgttaacgcc ggaaacgtgt tttccccatc
     8821 cggcaccagc gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg
     8881 cagctgaggg ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag
     8941 cagcgtttgc ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa caacgccctg
     9001 cgccgcaaag cctgaggcta attcatcgac gcgagcacaa agctcgcgcc agttaagttg
     9061 ctcgtcatta agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg
     9121 ccagtcagag aagatcataa caaccgctcc agtgcatcaa cttccacgac aggcagcgtg
     9181 ctacccggcc agcgacgtac ctgctgcgcc tgcatcagat ccagcgtgtc cagccctgga
     9241 atggtgtccg gcgttaacca ggcggcaatc cgcgccagtt gcgttaagcc taagctcgat
     9301 tcaatggaag aactgatcac cgccgtcagc cccagcgcgt gcgccgcctg tacctgctcg
     9361 cgtactttct ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct
     9421 tcagccacaa aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg
     9481 gtttcacggg caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg
     9541 atgcggtggc gataatccgg gttaacatat ttggcaaact gctggccttt cagcggtgtc
     9601 caggcgcgat ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagatta
     9661 accaccatgc cgtcgcgcac ggcttcgtac aatcccactt tgaccttcgc cactttctcg
     9721 cctggcatat cagcaagttt gaggatcaga tcgtcaggat cgccattaca cagtggtgcc
     9781 gcacggtaat tggccgcctg cgggagcgta tctgccagct ccgccaatgc acagcttacg
     9841 ccaaaggcaa cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc
     9901 caggcaagca gcacgctttg cgcctcttcc caggtttcct ggctgaagcc cggcagtggg
     9961 gagatctccc cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg
    10021 gtttttaacc gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat
    10081 acctgcgcgc tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct
    10141 ggttgaaagc gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt
    10201 tgcccgccag ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca
    10261 ggcagcgcag cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt
    10321 tttccagatc cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg
    10381 cgtcgtactg acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag
    10441 ccatgtagga agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga
    10501 agatggcatt atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc
    10561 cgatggagta gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct
    10621 ggaagtccag cacgttcaag tgatgtacgc cggaatcatc tttatagccg ccgtaatcac
    10681 cacgcacttt ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga
    10741 tcacgccgat attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg
    10801 tcagaggacg gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt
    10861 cggtggattt ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg
    10921 gtgcgtaaag cattgcttca tcaggataaa tcatattgtg tcctttagtc agaaacgcaa
    10981 gatctgcgcc agacttgcga taacgccagc gggattttcc cgatgcgcgt tatgtccggc
    11041 gcgaggaatg acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc
    11101 acgttcacca cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc
    11161 aggctggacg gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt
    11221 gctgcgcagc gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg
    11281 ttgataccag tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg
    11341 gcgatcggaa cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc
    11401 ttcgacaata accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg
    11461 tccgccaagc gagtacccca ccagccaaaa gtcaaggatg ttgtaactaa ccaaggtttt
    11521 acgcagtaag tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg
    11581 acctgggaga tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca
    11641 ttcgtggcaa tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc
    11701 gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc
    11761 gtttgcgcac catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc
    11821 caggcgtcgg caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc
    11881 tcgaacatcg cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca
    11941 cgctcgcttt gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca
    12001 attaatacca gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt
    12061 gcggagagat cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg
    12121 gcggtcgaaa gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct
    12181 gccggaagtt gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat
    12241 tgcccctgtt caggcagata gtcgcagatg cgatgcgcca gttgcgcttc gccaaacgca
    12301 tcacggcggg caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac
    12361 cagggctggc gtttttctgc cggatgcagc tccagccagt cggcaatatt ggcaattaag
    12421 cgacgtccgc gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac
    12481 tcttctggtt cacagcttgc ctgccattgc aggagccgtt tgcccgtcag gctgcttccc
    12541 agttgcacca caatttgcgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa
    12601 agatcggcac acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag
    12661 ccaagagttt gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca
    12721 accaccacgc cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga
    12781 cgaggcgctt cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc
    12841 cagctaagcc cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata
    12901 tggactcccc ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt
    12961 gcggggatat cccgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac
    13021 attcccggct ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg
    13081 gcggttaaca ggatcagttt ttctccggtt aacccagctt caatcagtgc cggatagaga
    13141 tttgctaccg ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc
    13201 agccccagcg ccagatgtcc cagtccacgc tcatcgaaat gggtgtggtg aatgaatgtg
    13261 gaattctccg ccgccgctaa cgttaacggt gtagaacgcg agccaggggc gatacagatg
    13321 tgtctgacgc cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat
    13381 gcgcttactg acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa
    13441 aaatagatta ttgatatgaa tcggtaatga tgcgactcat tactattcca tctgtaataa
    13501 agtacgcagc cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc
    13561 acggacaatg cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg
    13621 cagggaaacg cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca
    13681 ttctcgggtg aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc
    13741 agcaactgct gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag
    13801 tgaagtccag atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg
    13861 taaaacatcc agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag
    13921 catgttctcg cgctggtttt tatcatccgc catcagccac tctcctaact gctgcgcctg
    13981 cttatcatca ggattatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc
    14041 acgccgccgc cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc
    14101 catgtaaaaa tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc
    14161 gttgaccgga cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc
    14221 accttcggcg atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg
    14281 ccagtgttgt tctcgcatgg tggttaaatg taacccaggc aagggcttga tactcaccag
    14341 tgtggcgata aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag
    14401 cgtcagccgc agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa
    14461 attgccctgc gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg
    14521 gtgctggcga agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag
    14581 gacggcagct tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg
    14641 actggccagc cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac
    14701 ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc
    14761 cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac
    14821 tacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc
    14881 tagtaccagc ccaacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta
    14941 gtcatcagca cgataaactg cctgcttcgc ccgatagtaa taactgtctg aagcctggct
    15001 aacccgtttt ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt
    15061 ctgatcggcg ggatcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt
    15121 caggtcgtca tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg
    15181 taaaacctgt cactaactat agacaagggt tgttgattac gcctgaatta cctcgcgtgc
    15241 catcccaatg tgtgggatac catcttcttc atagacctcc gtcacaggga taaaaccgaa
    15301 actctggtag aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca
    15361 gtgatgcgta catgtttcga gtgttttact catcagttgc tgccccactt tttcgccacg
    15421 caacgcttca ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt
    15481 cagaatcctc gcatatgcca ccagttcatc atttttccaa ccaagaatat ggcgattatc
    15541 ccccgtcaaa tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac
    15601 cgcgcaacgc agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag
    15661 atcttgccat tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata
    15721 ttttttgaaa cgtgatgaac tcatggaatt aattttttta ggtacttcgg ccggtgtgcc
    15781 aacccgcacg cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg
    15841 actttggttg tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa
    15901 ccctggaaaa ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt
    15961 acccggcttg ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg
    16021 tccgcaaggt atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga
    16081 ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa
    16141 agtaaccgct tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca
    16201 tgataaaccg ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggtcc
    16261 actgtttcag gaattaaaag cgggcaaaac aatcaccctg gaagatggaa ggcagattaa
    16321 cggcgcagat tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac
    16381 cggcccctgc gatgccgcac ttgatctggc taaaggtgtc gatgtcatgg tgcacgaagc
    16441 gacgctggat ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca
    16501 ggctgcgaca ctggcccgtg aggctggagt cggtatgcta atcattaccc acgtcagctc
    16561 gcgctatgat gacaaaggtt gtcagcacct gttacgtgaa tgcaggtcaa ttttcccggc
    16621 gactgaactg gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac
    16681 agggcgaaac ttgccctgtt atcgcaaccc gcgctacata tatccaccca cacgggcata
    16741 ttgagcgatt tttgatattt attcatctcc tcacgcacta tattcagagc aataagttgc
    16801 ctctatttat cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag
    16861 ttgtcagcaa ttattgtcaa ttatctcaaa agcaactcag tcagacattt gcagaaaaat
    16921 ttactgtgac cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag
    16981 aaagcattga gttactgcat aatattgcgt taggttatga taaatttgga aaagaagctg
    17041 aagacattct ttaccatatt gttagaaacc caacaaatga gaccctatcg attatccgac
    17101 ttatcaaaaa tgcttgttta aaattatata atttggcaca taccgcaacc aactcccccc
    17161 ttaaaccaac tggtccggat aattcagatg ttctcctgtt caaaaagcta ttctcccctt
    17221 cgaaattaat gacaattatc ggtgatgaaa ttcctcttat atctgaaaaa cagtcgcttt
    17281 caaaggtgct tttaaatgat gagaataatg aactgagtga tggtacaaac ttctgggata
    17341 aaaatcgtca attaaccaca gatgaaatag cttgctatct tcagaagatc gccgctaatg
    17401 caaaaaatac tcaagtcaat tatcctactg gtctctacgt cccctactcc accagaactc
    17461 acctggaaga cgctctcaat gaaaatatta agagcgatcc atcatggccg aatgaagtcc
    17521 agttattccc cataaatact ggcggacact ggatattagt ttcgctacag aaaatagtaa
    17581 ataaaaaaat aataaactac aaataaaatg cgtcatattc aactcattgc gtgcactagg
    17641 ccatgataaa gaaaattcac ttacgcgtgt cattaatagt ttcaattctg aactcatggg
    17701 agaaatgtcg aataataata taaaagttca tttaaatgaa ccagagataa tatttttaca
    17761 tgccgatctt cagcaatact taagccaaag ttgcggtgcg tttgtgtgca tggcagccca
    17821 ggaagtgatt gaacaaaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa
    17881 ctatgctgac agatttaaaa aatattcagc agaagagcag tacgaaattg a
//
LOCUS       DAZFVO020000104        17576 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFVO020000104 DAZFVO020000000
VERSION     DAZFVO020000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.105"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHKNB_RS16370"
                     /old_locus_tag="pgaptmp_003274"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHKNB_RS16370"
                     /old_locus_tag="pgaptmp_003274"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HGU4196734.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHKNB_RS16375"
                     /old_locus_tag="pgaptmp_003275"
     CDS             complement(1575..1682)
                     /locus_tag="ACHKNB_RS16375"
                     /old_locus_tag="pgaptmp_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196735.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHKNB_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHKNB_RS16380"
                     /old_locus_tag="pgaptmp_003276"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HGU4196736.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHKNB_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHKNB_RS16385"
                     /old_locus_tag="pgaptmp_003277"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HGU4196737.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHKNB_RS16390"
                     /old_locus_tag="pgaptmp_003278"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHKNB_RS16390"
                     /old_locus_tag="pgaptmp_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HGU4196738.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHKNB_RS16395"
                     /old_locus_tag="pgaptmp_003279"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHKNB_RS16395"
                     /old_locus_tag="pgaptmp_003279"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HGU4196739.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHKNB_RS16400"
                     /old_locus_tag="pgaptmp_003280"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHKNB_RS16400"
                     /old_locus_tag="pgaptmp_003280"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HGU4196740.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPTFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHKNB_RS16405"
                     /old_locus_tag="pgaptmp_003281"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHKNB_RS16405"
                     /old_locus_tag="pgaptmp_003281"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HGU4196741.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHKNB_RS16410"
                     /old_locus_tag="pgaptmp_003282"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHKNB_RS16410"
                     /old_locus_tag="pgaptmp_003282"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HGU4196742.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHKNB_RS16415"
                     /old_locus_tag="pgaptmp_003283"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHKNB_RS16415"
                     /old_locus_tag="pgaptmp_003283"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HGU4196743.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHKNB_RS16420"
                     /old_locus_tag="pgaptmp_003284"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHKNB_RS16420"
                     /old_locus_tag="pgaptmp_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HGU4196744.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12764..14680
                     /gene="mngA"
                     /locus_tag="ACHKNB_RS16425"
                     /old_locus_tag="pgaptmp_003285"
     CDS             12764..14680
                     /gene="mngA"
                     /locus_tag="ACHKNB_RS16425"
                     /old_locus_tag="pgaptmp_003285"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HGU4196745.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14698..17331
                     /gene="mngB"
                     /locus_tag="ACHKNB_RS16430"
                     /old_locus_tag="pgaptmp_003286"
     CDS             14698..17331
                     /gene="mngB"
                     /locus_tag="ACHKNB_RS16430"
                     /old_locus_tag="pgaptmp_003286"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HGU4196746.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17356..>17576
                     /locus_tag="ACHKNB_RS16435"
                     /old_locus_tag="pgaptmp_003287"
     CDS             17356..>17576
                     /locus_tag="ACHKNB_RS16435"
                     /old_locus_tag="pgaptmp_003287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196747.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELI"
BASE COUNT         4261 a         4378 c         4776 g         4161 t
ORIGIN      
        1 ttacaatttt ttgtgtttag gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attacgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtact gcactgtgca
     3361 tcgaaactgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggcgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcagttc cacccgaccg
     3541 gtattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga tcgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgaccacct gggtaaagaa gttctcgaat
     3781 cccgcctgcc gggtatcctg gaactctccc gtaccttcgc tcacgttgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga
     3901 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg ggactgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgtgcag cgggtctgca tctgcaagag tctatcgccg
     4081 aacagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcct gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gactttccac gatctgaccg
     6001 aagcagactt ccaggaaacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccgctggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtcgcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgc tgcaacatat tcataacggc cagggcgcgt
     7561 tccgtgtctg ggactccgtt ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgactatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cctctggcga gcagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgtaccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagta atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgatccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttctacgccg cgcggcctgg tgacaccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca agctgaccgt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgcgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccataa agttgcgctt gatccgctga caggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc tggctctaac
    11461 tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtaatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcatgttacc
    11581 aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ttgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accattttct tcacttcttc aataaagtga tattttgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt aaataataca
    12661 aatacaatac aaataatttc aatcaagtga aattgatcac ataatggtat tgttttatcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgttg atggcccgga agcggttgat ttagtggtgc tgctggcgat
    13081 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
    13141 ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
    13381 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
    13561 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
    13621 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag
    13981 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcctgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggcaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac cagattcctt tggcatgtcc gggcaactgc cgcatatcta caatggattt
    15121 ggcattaccc gcaccatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc
    15241 tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt
    15301 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatgggcat
    15361 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
    15421 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat
    15541 cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
    15721 ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
    15841 agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt
    15901 aacaccactg tgcggctgcg cgccagccag tttaatctac gagacgatcg cggtcagcct
    15961 gtaccgtatt ttattcgcca tgcccgtgag atcgatccag gcctaatcga tcggcaaata
    16021 gttcattacg gtaattacga tccctttatg gagtttgata tacagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatc gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gatactggaa aatgctttct ggcaaattgc gctcaatgag
    16201 gatggaacgc tacaactggt agataaagat agcggtgtgc gctatgaccg ggtgttgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaatgactt ctgcaacagc gaaaccgcaa tgcgagatta ctcatgaggc ctggcagagc
    16381 agggctgtta tccgctatga catggcggtg ccgctcaatt tgttggaacg aagcgtccgg
    16441 caatccacgg gcagagtagg ggtggagatg gttgtcactc tgagtcataa cagcagacgt
    16501 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg cgtcctgatc
    16561 cctacatctt ttaacaccga tagtgttctg gcagataccc agtttggttc gctaacgcgc
    16621 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
    16681 ccggtatgga atatgctcaa ctatgcagcc ttacaggaag ggcgtaacgg catggctgtc
    16741 tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
    16801 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgtttttaag gcctgggcgg
    16861 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
    16921 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
    16981 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
    17041 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
    17101 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
    17161 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
    17221 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
    17281 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
    17341 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
    17401 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
    17461 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
    17521 ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt aattat
//
LOCUS       DAZFVO020000105        17394 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFVO020000105 DAZFVO020000000
VERSION     DAZFVO020000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.106"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(213..932)
                     /gene="dcuR"
                     /locus_tag="ACHKNB_RS16440"
                     /old_locus_tag="pgaptmp_003288"
     CDS             complement(213..932)
                     /gene="dcuR"
                     /locus_tag="ACHKNB_RS16440"
                     /old_locus_tag="pgaptmp_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HGU4196748.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQISGFQCCGTASTLEKAKEMIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(929..2560)
                     /gene="dcuS"
                     /locus_tag="ACHKNB_RS16445"
                     /old_locus_tag="pgaptmp_003289"
     CDS             complement(929..2560)
                     /gene="dcuS"
                     /locus_tag="ACHKNB_RS16445"
                     /old_locus_tag="pgaptmp_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGU4196749.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2741..2971
                     /gene="yjdI"
                     /locus_tag="ACHKNB_RS16450"
                     /old_locus_tag="pgaptmp_003290"
     CDS             2741..2971
                     /gene="yjdI"
                     /locus_tag="ACHKNB_RS16450"
                     /old_locus_tag="pgaptmp_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HGU4196750.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2983..3255
                     /gene="yjdJ"
                     /locus_tag="ACHKNB_RS16455"
                     /old_locus_tag="pgaptmp_003291"
     CDS             2983..3255
                     /gene="yjdJ"
                     /locus_tag="ACHKNB_RS16455"
                     /old_locus_tag="pgaptmp_003291"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4196751.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3482..3778
                     /gene="ghoS"
                     /locus_tag="ACHKNB_RS16460"
                     /old_locus_tag="pgaptmp_003292"
     CDS             3482..3778
                     /gene="ghoS"
                     /locus_tag="ACHKNB_RS16460"
                     /old_locus_tag="pgaptmp_003292"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4196752.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3806..3979
                     /gene="ghoT"
                     /locus_tag="ACHKNB_RS16465"
                     /old_locus_tag="pgaptmp_003293"
     CDS             3806..3979
                     /gene="ghoT"
                     /locus_tag="ACHKNB_RS16465"
                     /old_locus_tag="pgaptmp_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HGU4196753.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4098..5615)
                     /gene="lysS"
                     /locus_tag="ACHKNB_RS16470"
                     /old_locus_tag="pgaptmp_003294"
     CDS             complement(4098..5615)
                     /gene="lysS"
                     /locus_tag="ACHKNB_RS16470"
                     /old_locus_tag="pgaptmp_003294"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGU4196754.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5852..7309)
                     /gene="dtpC"
                     /locus_tag="ACHKNB_RS16475"
                     /old_locus_tag="pgaptmp_003295"
     CDS             complement(5852..7309)
                     /gene="dtpC"
                     /locus_tag="ACHKNB_RS16475"
                     /old_locus_tag="pgaptmp_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HGU4196755.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7368..9515)
                     /gene="cadA"
                     /locus_tag="ACHKNB_RS16480"
                     /old_locus_tag="pgaptmp_003296"
     CDS             complement(7368..9515)
                     /gene="cadA"
                     /locus_tag="ACHKNB_RS16480"
                     /old_locus_tag="pgaptmp_003296"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HGU4196756.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9595..10929)
                     /gene="cadB"
                     /locus_tag="ACHKNB_RS16485"
                     /old_locus_tag="pgaptmp_003297"
     CDS             complement(9595..10929)
                     /gene="cadB"
                     /locus_tag="ACHKNB_RS16485"
                     /old_locus_tag="pgaptmp_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HGU4196757.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11295..12833)
                     /gene="cadC"
                     /locus_tag="ACHKNB_RS16490"
                     /old_locus_tag="pgaptmp_003298"
     CDS             complement(11295..12833)
                     /gene="cadC"
                     /locus_tag="ACHKNB_RS16490"
                     /old_locus_tag="pgaptmp_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HGU4196758.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(13093..13252)
                     /locus_tag="ACHKNB_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /pseudo
     CDS             complement(13093..13252)
                     /locus_tag="ACHKNB_RS16495"
                     /old_locus_tag="pgaptmp_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13715..14041)
                     /locus_tag="ACHKNB_RS16500"
                     /old_locus_tag="pgaptmp_003300"
     CDS             complement(13715..14041)
                     /locus_tag="ACHKNB_RS16500"
                     /old_locus_tag="pgaptmp_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4196759.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(14038..14301)
                     /locus_tag="ACHKNB_RS16505"
                     /old_locus_tag="pgaptmp_003301"
     CDS             complement(14038..14301)
                     /locus_tag="ACHKNB_RS16505"
                     /old_locus_tag="pgaptmp_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HGU4196760.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(14373..15239)
                     /locus_tag="ACHKNB_RS16510"
                     /old_locus_tag="pgaptmp_003302"
     CDS             complement(14373..15239)
                     /locus_tag="ACHKNB_RS16510"
                     /old_locus_tag="pgaptmp_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4196761.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPSPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSRKA"
     gene            complement(15324..15521)
                     /locus_tag="ACHKNB_RS16515"
                     /old_locus_tag="pgaptmp_003303"
     CDS             complement(15324..15521)
                     /locus_tag="ACHKNB_RS16515"
                     /old_locus_tag="pgaptmp_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4196762.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(15533..16021)
                     /locus_tag="ACHKNB_RS16520"
                     /old_locus_tag="pgaptmp_003304"
     CDS             complement(15533..16021)
                     /locus_tag="ACHKNB_RS16520"
                     /old_locus_tag="pgaptmp_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4196763.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(16018..16395)
                     /locus_tag="ACHKNB_RS16525"
                     /old_locus_tag="pgaptmp_003305"
     CDS             complement(16018..16395)
                     /locus_tag="ACHKNB_RS16525"
                     /old_locus_tag="pgaptmp_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4196764.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            complement(16442..16816)
                     /locus_tag="ACHKNB_RS16530"
                     /old_locus_tag="pgaptmp_003306"
     CDS             complement(16442..16816)
                     /locus_tag="ACHKNB_RS16530"
                     /old_locus_tag="pgaptmp_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HGU4196765.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYL
                     ADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHSVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            complement(16979..17200)
                     /locus_tag="ACHKNB_RS16535"
                     /old_locus_tag="pgaptmp_003307"
     CDS             complement(16979..17200)
                     /locus_tag="ACHKNB_RS16535"
                     /old_locus_tag="pgaptmp_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4196766.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(17263..>17394)
                     /locus_tag="ACHKNB_RS16540"
                     /old_locus_tag="pgaptmp_003308"
     CDS             complement(17263..>17394)
                     /locus_tag="ACHKNB_RS16540"
                     /old_locus_tag="pgaptmp_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4196767.1"
                     /translation="PSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         4624 a         4005 c         4139 g         4626 t
ORIGIN      
        1 cacataatgc gggtgacata atagttaatt aacttttgtt agcgttttga aattaaaaac
       61 accgttcacc tgaagagata ttaattttta gcgatgatgg agggataatt atatttgatc
      121 tggcacaagt tttactgatg aaggatgtaa cttgtgccag gggtattttg cattacggta
      181 ataattatta cttacagata accagcgatc cgttattggc aatattgttt cagtagtgag
      241 tagtgttctg cctgaatacg gtaacggtaa actggacgcc ccgtgacgcc ataatggata
      301 ctggtgaaca agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc
      361 gaaatgttaa cctcgttggc tagctcgtcg gttgaaaact catagtcctg atgcgcgtca
      421 atccactggc acagtgtgcg taacgtctgc ggcgttaagc cttttggcaa gcgacgagga
      481 tcctgttcgt tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac
      541 tgatgttttt ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg
      601 gaagcctgga agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg
      661 gttgccgcat cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc
      721 aggacaggca gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg
      781 tcgataggcg tatcgctatt gaagatcatc tctttggctt tctccagcgt cgaggctgtt
      841 ccacagcatt gaaagcctga gatttgtgct acgtatcggc gattcagctc cgcgaccatt
      901 gcgtcgtcat cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta
      961 tctggacaaa aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga
     1021 gattttctac ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg
     1081 agacaccttt gtcaaaaatg tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt
     1141 taacttcgca gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg
     1201 gttccggccc taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg
     1261 tcgcgacctg gtcctcactg ccgctgtctg gcagctggct ttcactgttt aaaatcagcg
     1321 tatggcctaa atcggtcgcg cgattaatct tgctgattaa aaaaccagcg ataaccggag
     1381 atttgatctt acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa
     1441 tgtaatcttc caactgctta taactcttca gatgcaataa tccgagaatc acatgcaatt
     1501 tattcataaa ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc agaccgtcga
     1561 gtcgctgcat cagtttacga acttcagttt tgtccctgaa ggttgaaatg gcaccgatga
     1621 taacgccatt actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa
     1681 tctcttcgtc gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct accacctgtg
     1741 accatgagtg gcttagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca
     1801 attcttgtgc ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc
     1861 cttctttgat agactgcaac atggcctggc gctgctcaaa cagcgtggag atttcgtagg
     1921 gttccaggcc gaaaaggatt tttttcagta ccttaaccag aatgcaggtg ccaatcagtc
     1981 cgaccagcat gccaaataat accgaccaga taatgctcca gcgactgtca ttgatttgtt
     2041 gggtcacgcg gcttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat
     2101 cgtagatggg ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg atagcgacat
     2161 tttctttgcc attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct
     2221 gggcttcagg atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat
     2281 cgttgcgttt gcgcacggct tccgcgatgg cctggatgcc gctctcctgc ggttttttct
     2341 gcaagccctg acggatttcc ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt
     2401 tggctagccc gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca
     2461 atagcaccga gaacagtacc gcactgacca ttaagatcac tgtggtactc aatttcatcg
     2521 gacgtttgcg taacatgcgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa
     2581 tttcttaagc attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta
     2641 tataggggaa gagattctgg caacggaaac tgccagtgct gtatgaagat tacagggcta
     2701 tgcttatagc gataatcata ctgatgagag agggaaagtc atggatcagg cgctactgga
     2761 cgagggttat cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg
     2821 tcagcattct ggcaattgcg tacgtggcaa cggcaagtta tttaatctca aacgaaagcc
     2881 gtggatcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
     2941 gagcggtgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat acgcgaaggc
     3001 cacaataagt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
     3061 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
     3121 caggggattg gtaaacagct ggttgcaaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
     3181 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
     3241 gatattcgca gttgatggga gagtacagat tcacgatatt tttcatgctc tccgcggtgt
     3301 gatgcaggag agcatctgaa gggtaggggg atgcacaaag aatgggcaga gagagcgttt
     3361 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
     3421 atcaatcggg gttaataagt tttgcgtccc cagagcgttc aatattgata ggagtcatat
     3481 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
     3541 ttcctcagtg ttcttaaggt taagatcatt gatgtatgat atgaatttct cttctatcgt
     3601 ggctgatgaa tatgggatac cacgacaatt gaatgaaaat tccttcgcaa taacgacatc
     3661 attagccgca agtgaaatcg aagatttaat caggctcaaa tgcttagact taccggatat
     3721 tgattttgac ctcaacatta tgacagttga tgactatttc cgtcagtttt acaagtagcc
     3781 aggaaggata agaagaacat aaaaaatggc actattctct aaaatattaa ttttttatgt
     3841 gattggtgtg aacatatcct ttgtcattat ctggtttatc tcacatgaga aaacacatat
     3901 tcgtttactt agtgcattcc tggtcggaat aacctggcca atgagtctgc ctgtggcatt
     3961 acttttttct ctcttttagg agctgtagtt gagtggaccc gctggttatc gtgaaacgaa
     4021 gcgtctctcc tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc tgactgcttc
     4081 gttcattaaa gtgaaattta tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga
     4141 atagtatgac tgtttgtaaa cagcataatc attcggtcga taccaatacc cagaccagcg
     4201 gttggcggca gaccatattc cagcgcagtc acgtaatctt cgtcatagaa catggcttcg
     4261 tcgtcacctg cagctttagc gttaacctgt tcctggaaac gttcagcctg atcttctgcg
     4321 tcgtttaatt cgctaaaacc attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg
     4381 tcggtgattt ccgggttaac atcattacgg cgcgccagcg gtgacacttc tgccggatat
     4441 tccgtaataa aggttggctg aatcagatgt gcttctgcca cttcatcaaa gatctccgtg
     4501 acaatacgtc ccagccccca gcttttctct accgtaattc cgatagattc agctaatgct
     4561 ttagcagcat caaaattatc caggtcggcc atatcggttt ctggacgata ttttttgatt
     4621 gcttcgcgca tggtgagttt ttcaaacggt ttgccgaaat caaacacatg ctcgccataa
     4681 gtgactttag tggtacccag aacctcttgt gccagggtgc ggaacaggga ctctgtcagt
     4741 tcaatcaaat cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt gaactcagga
     4801 ttatggcgaa cagaaatacc ttcattacgg aagttacggt tgatttcgaa tacccgttca
     4861 aaaccgccta caaccagacg tttcagatac agctctggcg cgatacgcag atacatatct
     4921 aaatccagag cattatgatg ggtaataaac gggcgagcag atgccccacc tggaattacc
     4981 tgcatcatcg gggtttctac ttccataaag ccgcgcgcga ccatgaactg acggatagcg
     5041 gccagaattt ttgaacggac aacgaacgtt tgacgggatt tatcgttagc gatgaggtcc
     5101 agataacgtt gacgataacg gacttcctga tcctgcagac catggaattt atctggtaaa
     5161 ggacgtagtg ctttagtcag caggcgcagc tcagtacagt gaatggaaag ctcacccgtt
     5221 tgcgtcttaa acagcgtacc gcgggcaccg ataatgtcac ccagatccca ttttttaaac
     5281 tgatcgttat aaacaccttc tggcaggcta tctcgtgcga cgtacagttg aatacggcca
     5341 ccgacatcct gcaacgttac aaaggaggct ttccccatga tacgacgggt catcattcgg
     5401 ccagcaaccg agacttcaat gtttaaggat tccagttcct ggttatcctt cgcatcaaac
     5461 tcttcgtgca actggtcaga ggtatggtcg cggcgaaaat cattgggaaa cgccacacct
     5521 tgctgacgta gtgccgccag tttttcgcgg cgatttctca gttcatcgtt aaaatcaata
     5581 gcctcattgg ctccccgtgt ttcttgttca gacattttgg ttcctctaaa tccagctttc
     5641 aatttagcgt agataaagag acagatcggt acactgtatt atctgcctcg actattaata
     5701 actcagttta tcttatcgaa actaacgctt acctcaagtt gatatcacta ataaataatc
     5761 atcaaaatgg tttaaatgac ttattaatct cgatttaata gtgtgataaa ctgaaagtta
     5821 atttattcat aacctattgt ttttacgaca gttaatcgtt gctctcctgt atcatattcg
     5881 tcggcgtgct aaacagaaaa cgggtagcaa aggccaatac cacgatgatc gcgacacaag
     5941 ccaacgtcca ctctcccatc tgagaaaaga atcgctggta agctgcaatt gccataccgc
     6001 taatttgcga ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc
     6061 ctgttgccag catataaatc ccggttaata cgccagacat tttcagacgc gtgatttgtg
     6121 caatcgccac cgggtcaata aagagttcgg caaagcccat tagcgccaac ccggatatca
     6181 tcacgcccat tgacgcttga ccgtcagccg ctgcatgtcg ggcatcaaat gccaacaaca
     6241 taaagccaca agccatcagc agtaagccaa aggcaaactt cagccagacg cgcaatgttg
     6301 agttgccgcg gctttctggc gacgccagcc aggccagtac aaccccagcg agcatcaccg
     6361 caattgcatt caccgactgg aatagtgctg taggtacttc aatattgaat gcctgacgat
     6421 tcacaaagcg atcgataaac aagctgatgg tactgccgcc ctgttgtgcc agtacccaga
     6481 acaacgtccc gacaaacatc aacaatacaa tttgccaaag agcacggcgg tgttcgggga
     6541 atttgatcat catgcgggca atgatttgtg cggcaatgag gcaaacgatc gccagcaaat
     6601 atccggacca gtcgttctcc agcagcaggg taaaaaatac tggggctaaa cagagcatca
     6661 ccaccaacca gctccatacc ggtaaggcaa atttgacgct agtgagcgct tttttatcca
     6721 tactacgcgt ggactggaaa tgacgatgac cgcttaagaa aatcaacaaa ccgataaaca
     6781 tgccgccacc cgcaagggca aagccaacat gccatccata ccactgagca gccaggccgc
     6841 aggcgattgg ggctgcgata gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc
     6901 catcacgtcg atgatcgttc tcgtcgtaaa gctcgccaag caaacagctg atgtttgatt
     6961 tgaataaacc gtagccacaa ataatgattg ccagcgccag atacaggcta aaggttgaat
     7021 ttgtatcaat gcccagcacc acatgaccaa gggtcattaa cagcgcgccg gcaatcactg
     7081 cagtgcggtt gccgagcagg cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa
     7141 ccagagaagc atatgcgctg aacaggctga tggcatggtt atcatcaaaa ccaagctgat
     7201 gggtgagata gagaatgagt aaggcacgca tgccgtaaaa actgaagtac tcccagattt
     7261 ggatcgccac gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt
     7321 atgagcaaaa aagggaagtg gcaagccact tcccttgtac gagctaatta ttttttgctt
     7381 tcttctttca ataccttaac ggtatagcgg ccatcagcct gacggtatgc accgtgaata
     7441 tcagtttcaa agcccggata gtgagcgccg atttcacaca gcatctgcag gaactccaga
     7501 accggacggc tttcttcggt gatcatttca cccggcatta ccagaggaac tcccggcgga
     7561 tacggaagga tcatattggc gttaatacga ccgaccattt cgtcgaggta aacttcttcg
     7621 gtcataccgt gcagctcttt ctggaacgca gcatacggag tcattaccat cgtcggcagc
     7681 acttcaaatg cgcgatacat cagatccggc agattgtggt gaacaatcag tttgtggata
     7741 ttctgagcca gttcctgaat acgcatgttt tcatagaatt caggatcttc acgatacaga
     7801 gacggcagca tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga
     7861 gcacgcagca agctcagtgc tttggtctta tcgataccga tgctgaacag gaacagcagg
     7921 ttatacggac cggttttctc aacaacgatg ccatgttcgt cgaggtattt cgccacgatg
     7981 ctggccggaa taccaaagtc gctcatggtg ccgtcttttt ccatccccgg agtcagcagg
     8041 gtgactttga tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa gccgtgccag
     8101 gtgctgtcag aacgcagcgg ccagcattca gtcgtatcga tatgatccgg ctgccaaaca
     8161 tcaaagaacc agccatcaga ttccgttctc agacgtttga tctctttacg gaatttgatc
     8221 gcacgttcaa tggaaccgtt gatcagacgc ttaccagcat tacctttcat catcgccgca
     8281 gcggtttcag tggacgccac gataccgtag tgcggagaag tggtggtgtg catcatgtag
     8341 gcttcgttaa aggtttcttc gtttacgtca cctttaacgt ggatcatgga agcctgagag
     8401 aacgccgcca gcagtttgtg agtggactgg gtttcgtaaa tcactttccc ttctacacgg
     8461 ccaccgctca taccgcattt accttcgtaa atcggtgaga agttggtgta aggcacccac
     8521 gcggagtcaa agtggatgga tttcacatcc agtgttttct tgatgaagtc ggtgttgtac
     8581 agcagaccat cataggtaga gttggtaatt acagcatgta ccggccaggt tgcgtttggt
     8641 gtttctttca cgcgcttagc aatggtagcg tgctggaatt cactctgtgg gataccacca
     8701 agaataccgt aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc
     8761 atcaggtggg tcagcgattt gtggcagtta cggtcaatca gaatggtgct gcctgccgga
     8821 gcagagtaca tgccaacaat tttgttcgca gtggaagtac cgttggtcac catgtagctg
     8881 cggtctgcgt taaagacgcg agcgatatac tgttctgctt ctttgtgtgg accactgtga
     8941 tccagcagag aacccagttc agatactgaa atggaaatat cagatttcat ggtattcgga
     9001 ccaaagaaat catagaacag gctacctacc gggcttttct ggaatgcagt accgcccatg
     9061 tgaccaggag tacagaaagt atatttacct tcacgaacat atttaaacag tgctttagtc
     9121 agtggaggca gaatagtgtt gatatattcg tcagtggtct gcttgatctt attagcaata
     9181 tcttcagcag cacccagcgc atattcaaag aagctaatct gtaaacgcag gtcattcagg
     9241 cttacatcga gagtggaata cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt
     9301 ttgctaattt cttcgcacag ctcgagatta tatttatccc agtcaaaaat aacgccgcac
     9361 agacgcgcat tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc
     9421 tggaagttca gacgttcaag cgcgcgatga agttcacgga tgggttcttc tttaaaataa
     9481 acccccatgt gattcaatat tgcaataacg ttcatagtca tatctccagg taaaaaaggc
     9541 ccctcccaac acatgggaca aaatgaaagg aggagcctcg gaaaatactt ttaattaatg
     9601 tgcgttagac gcggtgtggt tatccattga gtggctctgg cgctcgtgca ttttgcgagc
     9661 gtagaacatc aggataatca ggctgacgat gaaggtacct gccagctcga aggagcttgc
     9721 gcccatcagc gcgatgaagc agaacacaca acccagtaca gagcagatca ggctgacaaa
     9781 gttgcggatg ttaacgcctt caaaacgaat caggtcaacg caagagtaga agtacggcag
     9841 catagtcagc agtactgcga taccggtcag ttcaccgaac aggtcagatg ctttaccacc
     9901 ggcagagttc atcagagtga tgaggatcat cagggcagtc attttcactg cagccagcag
     9961 cagacctttt ttcggaatac cgttgctgtc gacttcacca taaactttcg ggaagttacc
    10021 gtcgttagcg gcacgtacac ctgcctggcc taccaacatc atccaggagc ccagagaagt
    10081 caggcacgca aaggcggtga atgcagaaac cagcggcgca gcccagttac cgaggatggt
    10141 tgaagcactg attgcaaacg gagcaccgga agctgccatt acagaagatg gatacatacc
    10201 ggaaagcacc tgagtcgcag cgatgtaaac aatacctgct aaaccagtac ccagcatggt
    10261 tgccagcgga acggtacgtt tcgggttttt aaccatacca gtacttactg ctgcggattc
    10321 aacacccacg aaggcccaca ggcagagcag aatactttta atgatcgcat gaccatcagt
    10381 ggtatccgca gtattccagt tagctgcata agttgccgca tcaaaccaat gccagccaac
    10441 aatagcggtc attactaccg gaataagaac cagcaccaga ccaatagtgg ttaaacggct
    10501 tacccaagta ccgccgagca tatttacaaa ggtaaatacc cagacgatag caatacaggc
    10561 gatacccgcc ggaacaggat catttaatac tgggaagaag gtggaaagat aagataccgc
    10621 ggtaatacca atcgccaggt taccaatcca gttagcatgg taataaagaa cacctgtctg
    10681 aaaaccaaat gcaggggaaa tttctccggc ataagcaatt gggccacctt gttgcgggtt
    10741 ttttgttgcc agtcgggcat atacatacgc cagcgacatt gcaccaataa tagagataat
    10801 ccaaccccag atagcaatac caccgatact tgctaggttc gcaggtaata atgcaatacc
    10861 gctccccatc atattaccgg caacaacacc ggtacaggca aatagcccga tcttcttggc
    10921 agaactcatg ctcttctcct aatttcattt ttgaatttgg agtccgggtc atgatgtata
    10981 actatttcct gaccagacca aactggcgct aagaatactc acgaaaaaag gattaatcct
    11041 aaagattagg tgaaataaca caaaagtttc tgtaagtgag aacttgaggt tttttattaa
    11101 cacatcagga tcgcaagttg atatcatgaa aagataaaca ttcaatgttt acaatggatt
    11161 gcgggatatt ctctggttaa atttatgtaa taaaaattat gcggcaaata aattgccgca
    11221 acatattata ccaacagaaa catacaaaaa ctcaacaaca aatattcccg agcataaatc
    11281 aacccgggag ttacttattc tgaagcgaga aatttgtcga gataaggtac aacataagga
    11341 acagaagtct ggaatatacc attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa
    11401 ttaaaggcgg tgagatatgc atcagccgct tcccggttca tccccttcat ttcataaacc
    11461 ttgccaagca acacataatt tagccaggac atttcaagat caatgccagt atttatcgcc
    11521 tggtaagact catctgtttt accttttacc agagcactaa ccgcttttat ttgatatata
    11581 atggacaggt tattcaattc cggcagtgta acaatgttgt ctatttctgt gttcagtgct
    11641 gctaattgtt tttcatctaa aggatgttga gaatggcgca cgatatcaac taatgctttt
    11701 tctgctctcg cgtaggtaaa ttctggggat gattgaacaa tctcacctaa taattcactg
    11761 gcacgattca atgatttgtc atcgccatgc agtaaataat catgagcctg ataaaaatta
    11821 gttaataacg caccacgatg aggcaaaatt tgctggagcg tctcctgcat tcgttgtggc
    11881 cacggttggt ttaacgcttt tgataaactc tccagtaaat cattttgaat cgccagctga
    11941 ttaccgttag tgatgacata acgtttatcc agcatggttg aaccatctgc attgtctacc
    12001 aattttatcg acataaagca ttgttgagca cggtattggc gctgattaac aaaggcaata
    12061 gataatgttt taccggaact gctcggttca tcaatgttgt agttgatttt gtcatgcacc
    12121 ataaaggtgg agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa
    12181 gagagctgat acggggaact ccagctgtta caacttttat ttaccatatt aatgtcaata
    12241 tcgcgtggat tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc aagagttgaa
    12301 aacgctacca gtgctacaca gatacctaac gacaacagga aaaaaaacca tacccaaaag
    12361 gtagtgaatc gtttgctttt aactggggat tgttcaggtg gcgttgcggt gttttgaatg
    12421 ttaagactgt gggagggaga atctgtggca ggaaccgcct ctggtatagg gggaggcgaa
    12481 gatagcatta tttcctctcc ctcttcttcg ctgtaccaga taaccggcac cattaattta
    12541 tagccgcgct ttggtacagt agcgatatag acaggactat cttcatcatt atcttttaat
    12601 gacttacgta gttctgagat actctgcgtc acaacatgat tggtgacaat acttctcttc
    12661 cagacattat cgataagttc atccctgcta agtacttcgc cactgtgttg agcaaagaaa
    12721 accagaagat caattaatct cggctcaagg gtaagttgac gcccattgcg gctaatttgg
    12781 tttatggacg gagtaacaag ccattcgcca acgcgaacta caggttgttg cataatagaa
    12841 actcattcga aaagggaatg gtgcaatgat aattgccatg acctattttt accatcaata
    12901 gatgggttta tttacatgtt attggtgaat gcaagacgtt atttttacca gccataaact
    12961 tcttgattac atagtattac gaaaggattt tactgagaac cagaagtaat attccttacc
    13021 atcaaaattc atcatctttg ccaaagaaaa atgttcagaa aataatccat ggaaaattgt
    13081 ccggagcgct tactatttta atggattgtt agtctttgca tgagcaagcg gactgataca
    13141 tttctctttg ttctcattca gaaaatctca tcagtcggcg ttcagaccgg tgttgtgctt
    13201 tatggatgcg ttacgctcct gatgacgtca tttgacgttc aacagcatca cggggccgca
    13261 cgacatttca cgtcagttag tgctatagct caggaacaaa ttttcccaaa ttgggatatg
    13321 ctcgcaaatt gctggtgatg tggggagaat ctggttgagt tcggtagaat tgatttggag
    13381 gcagaacgct taaatcgtgg cgtcctgaaa cgaaaaacgg acctccgtgg aggtccgttg
    13441 atatgaattt ggtgcccgga ctcggaatga aactggttat ataaccaatt gttttgtatc
    13501 gattgttttg tctttgttgt tttcgaggcc accaatgagg cccccatgtg caattcgaat
    13561 tcttttgacg ctataatccc tcttttttgt gctttgctgt atcattttgt ctggttcgat
    13621 atgctgaatt gcggaatcga agtaacaaca aaatacagag atttataaaa tacctggcta
    13681 cctgtgttag ggagcgttaa cgcacagatt ttgtttatgg ctgccagaaa tgatctgatg
    13741 gtttgtcgtc tggctctgca agtaccctgt tgatatcccg ggaaccatga acgatacgtt
    13801 cgatacgaac ttcagtatcc agtaccctga acagcagaag gtaacgacca tacgaacaac
    13861 ctctgatccc agcccctaat tcgggtcttt ctctgtagag tactggcgat tcggctatca
    13921 acagacattg ttggtaaagc tcttcggtaa aactgattgc acggacagga ttatcctgag
    13981 cgatccagtc acctatagct tcaagatcgt gttctgctaa tggtgaaaca gtcagtttca
    14041 tattggctgt tcaccttctg ccatcttttt gtatttgtgc gtcagtcgcc cgaatacctc
    14101 ttcagcactt ttactttcac cgctgttgat gcctgcggta acggcgctct ggagtgattc
    14161 aagctttatc tgctgttccc gttcttccag agcacgaagt ccggcacgaa tgacttcact
    14221 tgtattgtta taccgaccac tttctatttg ttcgcggata aacgtttcaa agtaaggact
    14281 gagggataca ctggtaggca tggtgatttt ctcgccgatt gattgctatc aattaataat
    14341 agttaatatt cgcgaattgt aaaatacctc ttttatgcct tgcggctttt gttcactgtg
    14401 cttgccaggg cttccgggta gtgtctggca ataatatcat taagcctgtc aaccagttct
    14461 ggtttcctga acgtgatgtg tactgagcct ttcttaaagt atttaatact gaacatctcg
    14521 tcttcatagt cctctttacc ctgaacagac tggatatgat catccagacg aagggtgatg
    14581 ttttcccggt tgtcggggct cggtttgccg ctgaacaggt gtagcattct ttccagatcg
    14641 gcaagtcgat ctgcctgttg cccggtgata aaatgaaacc cccaccggtc ccacctcacc
    14701 aggttgttga cgataatttt actgccaaat ttacagggtg agttggtttt gtaattccag
    14761 ctcagtcccc tgaagacgtt gatcacccct cgctcaaaca cctcatcctt attctggtgc
    14821 agttgttcaa atgtgctcag aatgtttgcc gcactgattt ccggaaaatt atcgtattcc
    14881 agtgaccggt accatgtatc acgtgcctgt gcatccatta atgacagcat cccggaacgt
    14941 tgcatcaggt cacgccagat ttcgcggtca agattccggg taatggcttt cattgctgtt
    15001 tcgggtttct ccatcagcca gcaaccgcag cgaaaattct gtttcatggc ccagtccggg
    15061 gcggttttac ctccaatact ttctgtcatt gctgagattt ctgtcagttg ccggataagt
    15121 gtgtcgattt gcatcagtgt ggtctttcgt acagccagaa tgtgtccgat tgaggtggaa
    15181 cagataacgt ccgtatgccc gttgaccact tcaggacggg agattgtgat acctgacata
    15241 atcgtttttc ctttttgcag ataaatgaaa acgccagccc tgttcaggct ggcgtcagtg
    15301 gaatgaagta tggtgagtga tatttaccgg ttctggtgca gaagctgcag atgacgaaga
    15361 gtacggacat cctcccgggc ctgttcgata cagggcaaca gtgctgccga atccgtttta
    15421 tcctcatcgt tgtcaaagat aattcccgat tcgcagtcga tattgtcctg cagccacgca
    15481 atgaggatat ccagcgctgt ttcagtggtt aatgatttca tgttgtttct ggttaccagt
    15541 tgaaagtggg taaacccggc aggcattcag cgctggcatc cagatgaatg agactgacgc
    15601 cataacgccg gataagtgtg acaaccagac ggcggaacat ttttgacagc cccaggcgtt
    15661 taagccgcag aaccgggtat gaccacgcat cggtacgtaa cagatatccg ctacctgtaa
    15721 aatgaatcca ttctgattcg ccgaagtcac tggtctggtg tgacagcgag tacaaccagg
    15781 cgttgtctgt ttccgtgata tgtgcggtgc tgcagcgtat gccggtaagg gttgttaacg
    15841 gtggtggtgt gcaggttgac tgttggtcgg agtcgtccac cacatggagt gaataaccgt
    15901 tttcagcgac tttgttaatc agttcagcga gctcaatgcc atcgacgtca acgacaatgc
    15961 gccccatgtt cagtgcctgc acgttaatgc tgtcggcttc cagtgtcagg gccagtttca
    16021 ttgttgcgcc tccggatgct taccctgggt aatgttattt accattctgt aattgtcgcg
    16081 ggtcatcagg cctgttgccc ggcgagcccg gaggatatcg atgctgttta ttaactgaga
    16141 gcgggtacag gcgctgaatc ctggctggtc agttcgtacc agcgcgtatt tttccacgag
    16201 aaagttcacc gcatcacaca gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc
    16261 atcggcaaac ggtgtgtcat tcagtgtgag gccataatgc tggtccagca gtcggatgag
    16321 cagtgtctgc cagatggtga cgggagacgg gcagcgagat gcctcccgta catgtgtgtc
    16381 ggataatgtt ttcatcttaa gttctctgta actggtagtg ctaaaggtga gagcttcggg
    16441 gttatgaggt ggttgcggtt gccggtgtcg gataaacagt cagataaacg taaccacagg
    16501 agccgagggt gtcggcttcg caggtcagcc cttttgcata cagcgtgacg gaatgctgat
    16561 gtcggggatt cagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa
    16621 aggcctggtc cagatggtat gcatctgcgt cgctgaagcg gccgctgata ccggcgcggt
    16681 cggcaaggta atgcaaccgg ttaccctcct gcaccagacg ggcgccaaaa cagggtttca
    16741 ctgtgcaggg aagcccccac caggggcggt cgtgattgtc gtcgggatga gttatcccgg
    16801 agagtttgtc tgacacgata aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg
    16861 acaggtcagc cggggcactg accggtttcg gtgaagatgt atgcattgat gagaatggcc
    16921 tcatccacag cggcgtctga ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc
    16981 agttcagggt gacgctcatc agccggacat acgggccaaa actgtcctta cggcgttcgg
    17041 caaacacggc cagcacaccg ggaatatcct gtacttcacg accggtatac gcttcagtgc
    17101 tgccttgcca gcggtatttg ccggtgcaga acggaaaaag acgggatgca ggatgctgcc
    17161 ggtgaatgcg catggcttca ccacgggtga taattttcat aatgggatac ctctgaagac
    17221 agaagataaa agtgaaaaca ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc
    17281 ggcaaaggag aaaacctggc tgccaccgac tatcagatgg tccggcaccc ggatatccac
    17341 caggcccagt gcctgtacca gacgttccgt gataaggcgg tctgccttac tggg
//
LOCUS       DAZFVO020000106        17321 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFVO020000106 DAZFVO020000000
VERSION     DAZFVO020000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.107"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="ACHKNB_RS16545"
                     /old_locus_tag="pgaptmp_003309"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="ACHKNB_RS16545"
                     /old_locus_tag="pgaptmp_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HGU4196768.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="ACHKNB_RS16550"
                     /old_locus_tag="pgaptmp_003310"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="ACHKNB_RS16550"
                     /old_locus_tag="pgaptmp_003310"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HGU4196769.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1667..1780
                     /locus_tag="ACHKNB_RS16555"
                     /old_locus_tag="pgaptmp_003311"
     CDS             1667..1780
                     /locus_tag="ACHKNB_RS16555"
                     /old_locus_tag="pgaptmp_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HGU4196770.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2041..2607
                     /gene="yceJ"
                     /locus_tag="ACHKNB_RS16560"
                     /old_locus_tag="pgaptmp_003312"
     CDS             2041..2607
                     /gene="yceJ"
                     /locus_tag="ACHKNB_RS16560"
                     /old_locus_tag="pgaptmp_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4196771.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGTQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2611..3186
                     /gene="yceI"
                     /locus_tag="ACHKNB_RS16565"
                     /old_locus_tag="pgaptmp_003313"
     CDS             2611..3186
                     /gene="yceI"
                     /locus_tag="ACHKNB_RS16565"
                     /old_locus_tag="pgaptmp_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HGU4196772.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3228..4280)
                     /gene="trhO"
                     /locus_tag="ACHKNB_RS16570"
                     /old_locus_tag="pgaptmp_003314"
     CDS             complement(3228..4280)
                     /gene="trhO"
                     /locus_tag="ACHKNB_RS16570"
                     /old_locus_tag="pgaptmp_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGU4196773.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4505..5425
                     /gene="lpxL"
                     /locus_tag="ACHKNB_RS16575"
                     /old_locus_tag="pgaptmp_003315"
     CDS             4505..5425
                     /gene="lpxL"
                     /locus_tag="ACHKNB_RS16575"
                     /old_locus_tag="pgaptmp_003315"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HGU4196774.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5597..6823
                     /gene="mdtG"
                     /locus_tag="ACHKNB_RS16580"
                     /old_locus_tag="pgaptmp_003316"
     CDS             5597..6823
                     /gene="mdtG"
                     /locus_tag="ACHKNB_RS16580"
                     /old_locus_tag="pgaptmp_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HGU4196775.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6906..7280
                     /gene="msyB"
                     /locus_tag="ACHKNB_RS16585"
                     /old_locus_tag="pgaptmp_003317"
     CDS             6906..7280
                     /gene="msyB"
                     /locus_tag="ACHKNB_RS16585"
                     /old_locus_tag="pgaptmp_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4196776.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7281..7508)
                     /gene="yceK"
                     /locus_tag="ACHKNB_RS16590"
                     /old_locus_tag="pgaptmp_003318"
     CDS             complement(7281..7508)
                     /gene="yceK"
                     /locus_tag="ACHKNB_RS16590"
                     /old_locus_tag="pgaptmp_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HGU4196777.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(7681..10224)
                     /gene="mdoH"
                     /locus_tag="ACHKNB_RS16595"
                     /old_locus_tag="pgaptmp_003319"
     CDS             complement(7681..10224)
                     /gene="mdoH"
                     /locus_tag="ACHKNB_RS16595"
                     /old_locus_tag="pgaptmp_003319"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HGU4196778.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10217..11752)
                     /gene="mdoG"
                     /locus_tag="ACHKNB_RS16600"
                     /old_locus_tag="pgaptmp_003320"
     CDS             complement(10217..11752)
                     /gene="mdoG"
                     /locus_tag="ACHKNB_RS16600"
                     /old_locus_tag="pgaptmp_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HGU4196779.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12146..13303
                     /gene="mdoC"
                     /locus_tag="ACHKNB_RS16605"
                     /old_locus_tag="pgaptmp_003321"
     CDS             12146..13303
                     /gene="mdoC"
                     /locus_tag="ACHKNB_RS16605"
                     /old_locus_tag="pgaptmp_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HGU4196780.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13311..14792)
                     /gene="clsC"
                     /locus_tag="ACHKNB_RS16610"
                     /old_locus_tag="pgaptmp_003322"
     CDS             complement(13311..14792)
                     /gene="clsC"
                     /locus_tag="ACHKNB_RS16610"
                     /old_locus_tag="pgaptmp_003322"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HGU4196781.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRMFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14734..15267)
                     /gene="ymdB"
                     /locus_tag="ACHKNB_RS16615"
                     /old_locus_tag="pgaptmp_003323"
     CDS             complement(14734..15267)
                     /gene="ymdB"
                     /locus_tag="ACHKNB_RS16615"
                     /old_locus_tag="pgaptmp_003323"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HGU4196782.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15362..15673)
                     /gene="ymdA"
                     /locus_tag="ACHKNB_RS16620"
                     /old_locus_tag="pgaptmp_003324"
     CDS             complement(15362..15673)
                     /gene="ymdA"
                     /locus_tag="ACHKNB_RS16620"
                     /old_locus_tag="pgaptmp_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HGU4196783.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15794..16126)
                     /gene="csgC"
                     /locus_tag="ACHKNB_RS16625"
                     /old_locus_tag="pgaptmp_003325"
     CDS             complement(15794..16126)
                     /gene="csgC"
                     /locus_tag="ACHKNB_RS16625"
                     /old_locus_tag="pgaptmp_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HGU4196784.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16185..16640)
                     /gene="csgA"
                     /locus_tag="ACHKNB_RS16630"
                     /old_locus_tag="pgaptmp_003326"
     CDS             complement(16185..16640)
                     /gene="csgA"
                     /locus_tag="ACHKNB_RS16630"
                     /old_locus_tag="pgaptmp_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HGU4196785.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16681..17136)
                     /gene="csgB"
                     /locus_tag="ACHKNB_RS16635"
                     /old_locus_tag="pgaptmp_003327"
     CDS             complement(16681..17136)
                     /gene="csgB"
                     /locus_tag="ACHKNB_RS16635"
                     /old_locus_tag="pgaptmp_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HGU4196786.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4336 a         4420 c         4105 g         4460 t
ORIGIN      
        1 agaaatttat ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 cggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc
      601 atatgccacc gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt
      661 atggtgaagg cgaaaagtat gtcccactgg tactccgcgc gcaaacactg tgggatgagc
      721 tctcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 tcgaaaagct cgacgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcagct gttcaactgc ccggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggagag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc
     1141 agcccgtacg caaagtattt gcctggtatc aggccgatgg tcgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccgaccacga taatacactg ttcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttaa cgccattcag gctttcccgc ttccaataat
     1621 catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt
     1741 gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac
     1801 tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tattcctgct
     1861 tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga agattctttc
     1921 attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt atttttgtgt
     1981 tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtttt ggatgcactt
     2041 atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta
     2101 agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat
     2161 tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttgttaatg
     2221 atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg
     2281 agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt gtacctattg
     2341 ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc
     2401 gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcacaca ggcagacttt
     2461 gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga
     2521 tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga
     2581 aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc
     2641 gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa
     2701 ggtcagcacg cctttgttaa tttccgcatc cagcaccttg gctatagctg gttatacggc
     2761 acctttaaag atttcgacgg tacttttacc tttgacgaaa aaaatccggc tgccgataaa
     2821 gtgaatgtga caattaacac caccagcgtc gatactaatc acgccgaacg cgataaacat
     2881 cttcgcagtg cagatttcct caataccgca aaatatccac aggcaacatt cacctccacc
     2941 agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct gaatggcgta
     3001 accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc atggggtggt
     3061 aaacgtgcag gcttcgaggc cgaaggcaaa attaagctca aggacttcaa tatcaagaca
     3121 gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag
     3181 aagtaagcgt gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga
     3241 tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaagat cttattgcca
     3301 ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag
     3361 caaatctcac tacaacaacc tttgtatttt tccgcgcata ctggacactg aataaacagc
     3421 aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcgcc gcactggtgg
     3481 caatgcgcga taatctcatc cgatatacgt tcgcccatcc gctcgtcaaa aacaaaattt
     3541 ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt
     3601 ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt
     3661 tcacaacgaa tgccgccggt gcagtacatg acgatttttt tatctttatg tgcctgcatc
     3721 atctcgactg cttttggcag ctgctcacgg aaggtatctg ccggaatttc gagcgcgttt
     3781 tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag tacatcggga
     3841 tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca
     3901 aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc
     3961 cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg
     4021 tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgcaggtac gctgatttgc
     4081 gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc
     4141 tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa
     4201 atggtggttc gcggttcgct ctcagccaac attttggctt ttagcgcgtc gttggaaatg
     4261 cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg
     4321 gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt
     4381 aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagcc acaaattttg
     4441 gttgcgggcg aaaaaatgcg acaatacata caattgcccg aataggttga aaaacaggat
     4501 tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac
     4561 ctggttgggt attggcgtac tttggttagt cgtgcaattg ccctacccgg ttatctaccg
     4621 cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt
     4681 gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt
     4741 ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg
     4801 gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt
     4861 gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac tggaactggg
     4921 cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc
     4981 actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg
     5041 caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc
     5101 ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttatttgccg ttgagcaggc
     5161 tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt
     5221 cgttccacgc cgtaagccag atggcaaagg gtatcaattg attatgctgc cgccagagtg
     5281 ttctccgcca ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag tggtcgaaaa
     5341 atgcatcatg atggcaccag agcagtatat gtggttacac cgtcgcttta aaacacgccc
     5401 ggaaggcgtt ccttcacgct attaaatctc ccctgccgga tgcttcagaa tggcatccgg
     5461 cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg
     5521 aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca
     5581 ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa
     5641 cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc tggtaatgcc
     5701 cttcttaccc ctctacgttg agcagcttgg cgttaccggt cactccgccc tgaatatgtg
     5761 gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg
     5821 tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgccc tcggcatggg
     5881 catcgtgatg gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctacgggc
     5941 gcttcttggg ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt
     6001 accgcgtaat aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagtggtgc
     6061 gttgctcggc ccaatggctg gcggcctgct cgccgatagc tacggcttac gtccggtatt
     6121 ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctgttct gcatcagaga
     6181 aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact
     6241 taaaaacccg aaactggtac tcagcctgtt tgtcaccacg ttaatcatcc aggtggcgac
     6301 aggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa
     6361 cgttgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctaagcgc
     6421 accacgactc ggcaaacttg gcgatcgaat cggacccgaa aagatcctga ttacagcgct
     6481 gatcttttct gtactgctgt tgatcccaat gtcttacgtt cagacgccat tgcaacttgg
     6541 gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact
     6601 gttggtttac aactcgagca accagatcgc cgggcgtatc ttcagctata accaatcgtt
     6661 tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactatgg
     6721 tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg
     6781 gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac
     6841 ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa
     6901 tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc
     6961 ttgcagacaa ccccggcatc gacgccgaag atgcgaatgt gcaacagttc aatgcccaaa
     7021 aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg
     7081 aagaaggtga atgtttacct atgcttagcg gtgaagccgc gcaaagtgtt tttgatggcg
     7141 actatgatga gatagagata cgccaggagt ggcaggaaga gaatacatta catgaatggg
     7201 acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg
     7261 atgagtggga tgaacgttaa tcactcatac gggccatgat gagtgtcgat cggcagcagt
     7321 aaagtatcaa aaaccagaga gaatggcaga tcaaggatcg tgacataacg ccaggcggag
     7381 tcacgcacat cccattgcac accgggataa tattggttgc catgcccctg ccccggtata
     7441 gtgcgactaa taatgctgcc acagccactc agcaaggtca ccatgatgct caccacaatt
     7501 aaacgcattt gatacctctt tcgtaaaaaa ataccggcat aacgccggta tttttattgg
     7561 ctcgttgttt ttgccggatg cggcgtgaac gccttatccg acccacaaaa tcttgcaaaa
     7621 tcaataaatt gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt
     7681 ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta
     7741 ataactcacc catgaagaat atctctccgg ggaattccag acgcggaaat gcagacgggc
     7801 catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt
     7861 ctcgttcagc gcctgttcaa cgtggcggtc acgggcgatt tccagcacct tactggcgcg
     7921 gtgacgcgcg gtcgccattg cggttgccag agcgttaaat gacgggttaa acactgcgtg
     7981 cataaagcca tcatcaaggg agcgttgacg attcatctca aggaaccgat cggtatcaac
     8041 cagcacctgc ggcggcgaat actcttccgg gatcaggaac agtttccagc gtttggtgcg
     8101 cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga tcaacgagaa
     8161 gacaatcggt gccagccaga acaggaaacg cagatccagc cacgccatcc caacagccca
     8221 cactaaccct aacagcagct gtgagccgtg gcgtttgaac gcttcacccc aggaagtgga
     8281 gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg cgctgacaac
     8341 gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaaa gcacttccag
     8401 cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt tgcaccagat
     8461 aagcaaaatg ctcaataact tcggcaggaa caacagcacc atagtcgaag caaaaagtgc
     8521 aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg gttgcaggaa
     8581 gtattgcggt tcggtcaacg catgtactac ctgcaatgca gtagagagcg cgaggaacat
     8641 aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaacaccg cacggtgaac
     8701 cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg
     8761 gcggtcacgt tttagctcat caagcaagtt aggcggtaat tcttcataag aacccgggag
     8821 atcgtaagca atccagaccc cccaacctgc acggcgcatc aacgccgctt ctacaaagtc
     8881 atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggagcca gtgcgcagtg
     8941 ctcgataaac ggtttcacgc ggataatcgc gttatgtccc caatagtgcg actcgccaag
     9001 ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg
     9061 acagcgcgca tacagcgtat ccataccgga cgctttcggc gacgactgaa tgatcccggc
     9121 gttcgggttg gcttccatca ggcgcaccag cccgcacaaa caatcaccgg tcattaccga
     9181 gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc
     9241 gatattaccg cttttacgct tcacgcggcg acggcggcgg cgatagaaaa tctgaccttc
     9301 gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg
     9361 gttataactg tcactaagaa tgtagacatc aaagtgtttg gcattcccgg tggcttttac
     9421 tgattcccac gttgcacgca ggccagcaaa aacacggttc acgtcttcgt tacagatagg
     9481 catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga
     9541 tatactgtat ttatcgcgac caataagcag ttgcaggaag cccattaacg ccgtccagaa
     9601 tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat
     9661 ataaggcaga agctgcataa aggaaaccca caaatcctga ccaaccatat ccataggatt
     9721 aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg cgacgacagt
     9781 ttgcgcgagc gtcaggatca acagaatgta acggcggatg gtaccgacgg tacgccactt
     9841 ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggcg tgacatcgcg
     9901 tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat
     9961 cgaggagcgt tttgcttctg gcatcgcctt cagctgatcg cgcccttcgt cgtctttaat
    10021 taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc
    10081 ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg cctgatgaac
    10141 ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc
    10201 aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg
    10261 tctgatcggc attcaccagc gcagcacgca tttcagtggt tttcttggca tctttcactt
    10321 tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc gtgctttcaa
    10381 ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatcc tctggcagtt
    10441 ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgaa
    10501 tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat
    10561 ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct
    10621 ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttggttt
    10681 catcgttggt tggaatttcc accagctcaa cgctgccttt accccactcc cctttcggag
    10741 tcacccatgc gcttggacga agatcgtaac gatcatcgag atcttcaaag cgggagaaat
    10801 cacgaccgcg ctgcaacaga ccgaagcctt gcgggttctc catcgagaag ctgctgaccg
    10861 ctaaatgttt cgggttattc aacggacgcc agatccattc gccattaccg gcatggatag
    10921 agagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg
    10981 gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca
    11041 gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataact acgaatttgt
    11101 aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagtcg
    11161 gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg
    11221 cggtatcaat tgccaggccg cgggcagaaa ggccataaac ctgacctgca ccaatcacgc
    11281 ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg
    11341 ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg tcatgctgaa
    11401 catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca
    11461 cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga
    11521 atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt
    11581 ccgcgtattt catatcgcgg aaaacggagg gcaagttgct tttgggcgcc tcatagcctt
    11641 tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag
    11701 atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc atttgtagtt
    11761 tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt
    11821 taatggataa ccagccattc cgacaacaga atcccggtat cgttcagcac gctggctcat
    11881 ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca
    11941 tcgacttcgt gtagggtttg ccagcaattt tatttatcaa ccgtcttaca gacgcttcca
    12001 ttctggttag tggtagcgat gatgctatgc ataaaaacct tgaagcctgc gcctggcgcg
    12061 gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata acccgacagg gataagacga
    12121 aaaatccgag attagttaac catatatgaa tccagtaccc gcgcaacgtg aatatttcct
    12181 cgactccatc cgcgcctggc tgatgttgtt agggatccct tttcatattt ctttaatcta
    12241 ttcgagccat acatggcatg tgaatagcgc cgaaccatca ttgtggctga ccctttttaa
    12301 tgacttcatc cactcgttcc gcatgcaggt atttttcgtt atatccggct acttttccta
    12361 catgcttttt ttacgctatc ccttgaaaaa atggtggaaa gtacgtgtcg aacgtgtagg
    12421 tatcccgatg ttaacagcca tccccctact gacattaccg caatttatta tgctgcaata
    12481 tgtcaaagga aaagcggaaa gttggcctgg gctgtcattg tatgacaaat ataatacgtt
    12541 ggcctgggaa ttaatatcac acctgtggtt tttactggtg ttagtagtca tgacgacgct
    12601 gtgcgtatgg atatttaagc gcatcagaaa taatttagaa aattctgata aaacgaataa
    12661 aaaattctcg atggtaaaac tatcggtgat ttttttatgc ctcggcatcg gttatgcggt
    12721 aataagaaga acgattttta ttgtgtatcc gcccattctg agtaatggca tgttcaattt
    12781 tattgtcatg caaacgctgt tttatttgcc gttctttatc ctcggcgcac tggctttcat
    12841 tttccctcat cttaaagcct tgtttaccac gccgtctcgt ggctgtaccc ttgcagcagc
    12901 attggcgttt gtcgcttatt tactcaacca gcgctatggc agtggcgatg cctggatgta
    12961 cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg atggtgaatg tggtcttctc
    13021 ctttggccac cgtttgctta acttccagtc agcgcgggtg acttattttg ttaacgcatc
    13081 gctgtttatc tatctggttc accacccgtt aacgctgttt ttcggcgcat acattacacc
    13141 gcacatcacc tccaactggc ttggttttct ctgtggcctg atatttgtag tagggattgc
    13201 gataattctg tatgaaattc atttgcgcat cccgttactg aagtttttgt tctctggtaa
    13261 accggttgtt aagcgtgaga acgataaagc accagcccgt taagccacat ttacaataac
    13321 cattccacgg gcaatatcga cgccagtctg accataaccc gcttccagaa actggtggcg
    13381 ggttcttttt tgagaataat ctctttcttt gcatgacgat caacccagtt gatccgtccc
    13441 cacctgtcca gacggagctg ccaggccgca tcatactggc tctgaataaa gcgtttatca
    13501 attaactgtg ccagcgtttc gctctctatc acgaagccca tttcagtatt gagcaatgtt
    13561 gaacgcggat cgaaattgaa agagccgata aacaccgttt taccatcgat gctaaaggtt
    13621 ttagcatgca ggctggctcc ggaattaccg gttatgccgc gatcgtgtaa cgtactactt
    13681 tgttcacgcg tcggcttgag ttcatataat tccacgccat agcggagcaa ttttttgcgc
    13741 cagcgcgcgt atccggcatg gacgacagca acatcgttag cggcaagaga attggttagg
    13801 atcgcaatct ttaccccttt tctcaccatc cgtaagagtt gcgccacacc tgcgcgtgtc
    13861 ggtacaaaat aggaagagat aatatcgatg cgttcactgg gtgagcccat gatatcgaac
    13921 atgcgctgcg gtaacagtga atgccgtttt gccttgccct cccctttcgc cggatcatca
    13981 cttaataaac gtgttttcgc ccagataagc ggcaacgttc catcaaccag atgatttata
    14041 aacggactgg attccatttt gcgtaagtaa cgatgcgtca tagcatcgtt atgccaggag
    14101 gcgggtaact cgatgcgatc cgccatttca ccttccggga catccagcac ttgctgtaag
    14161 ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat cggcaacgtc ctctaccacg
    14221 ggccctattg ccataacatc taaatccgaa aaaagtggct cttcccctgc cccaaaatag
    14281 gcatcaccaa tatttcgccc ccctaccagc gtcaccacgc catcgacagt gaaactttta
    14341 ttgtgcatac ggcgattgag acgggaaaag tcggtgatat aaccaagcgg acgcagcaag
    14401 cgaaacgaga aaggattaaa aagccggact tcaatgcggg gatggctgtc aagcaagcgt
    14461 aaaatgtcgt caagtccggg cgtattgttg tcgtccagca acaaacggac gcgaacgcca
    14521 cgctttgctg cggctaacag ggcggaaaac agtaaccgac ccgacatatc atcctgccag
    14581 atgtaatact gaacatccag cgtatgctct gccatttcgg ccagacgata ccgggcggca
    14641 aacgcatcca gacttttctc cagagggaaa agaccacact gaccgggatg ttgcgaacac
    14701 agtggcagca ccgcgctcgc cagccggggc aaatcattca tctccttgtt gggtaaggag
    14761 tctttcgtag aggtgggcgt tttcttcatc ataacagaca aagtatacct gttcgggtaa
    14821 agcgtgacgg gtaataaatt ctgaaacggt tttaactgcg atttcagccg ctgccgcacg
    14881 agggtaacca taaaccccag tactgattgc aggaaaagcc actgacgtat agctgtttgc
    14941 cgccaccagt cgcaggctat tcagataggc atcctgcaaa agctggtctt cgttttgttc
    15001 accaccacgc cagactggcc cgacggtgtg tactacggct ttagcgggaa gatcgcctgc
    15061 aagcgtaata acggcatgcc ccgtagggca atcgccctgc tgttgcctga cttttaaaca
    15121 ggcatccagc agggccggac ccgctgcgcg atgaatggcc ccatcgacgc cgccgcctcc
    15181 cattaatgac ggattagccg cattcacaat cacatcaacg gccagtttgg taatatcacc
    15241 ctgcacaaca tgaatacgcg ttttcataac gcctccttac accttcttgt ttcttaagtg
    15301 tacagcaggc tttttggtta aatcgcattt ttctgctcag caacgtgcgt tgttcagaaa
    15361 cttagttata ttcgatgttc attacagcca gctttttctg tttatcaaga tactgcactt
    15421 ttactgaggc tatttgcgcg tttttaacat taccgctcat taacgctttg ctggaatagg
    15481 ttttgcccgg aacagaacca ttctgtgggc agttcatttc tacgggtgat gactgggtgc
    15541 tgacgtcaca tgccggttcc acaatttggc cataaaaatg gatcaccccc ccttgcgcgg
    15601 tgcttcctgc gatggcaata aaaggaaaaa acaaactgcc gagcataaga gagtttaaaa
    15661 aaggtctgaa catttattct atttcctcaa tgattagtca tccttgaggg ttgtgttatc
    15721 catactttca ggttggctga ttcgcagcag accattctct ccagattcat cttatgctcg
    15781 atatttcaac aaattaagat ttttctgaag agggcggcca ttgttgtgat aaatgaagtg
    15841 actgtccatc agaaacagta acaactattt tcacccgatc gtccggggaa atatttaaac
    15901 tcaacttcgt caaagcaatg ggttgattag caggcaatga aagggtcttt tcttgcttcg
    15961 tctgactttg ccctgaactg ccttcgcgca gggacaatat ttgtactctg cacagacaag
    16021 attgagtaag agtgacttca ggaataatgg tatacacatc cccttgctgg gtcgtattaa
    16081 aggttatctg gctggaaagt gccgcaagga gtaataacgc attcatattc ttctcccgaa
    16141 aaaaaacagg gcttgcgccc tgtttctgta atacaaatga tgtattagta ctgatgagcg
    16201 gtcgcgttgt taccaaagcc aacctgagtc acgttgacgg aggagttaga tgcagtctgg
    16261 tcaactgcag caccgttgcc accaccgaac tgtttaaccg tcatttcaga atttttgccg
    16321 ttccactgat caagagtagc gctgttaccg aagccacgtt gggtcagatc gattgagctg
    16381 tcatccgagc cctgaccaac atctgcacca ttaccgccgc catgctgggt aatagtcaag
    16441 tcagagttac gggcatcagt ttgcagagca agtgcagagt taccgccacc gtactggtaa
    16501 atgttcagct ctgaatttgg gccgctatta ttaccgccac caccgtggtt accgccgccg
    16561 ccgtactgag gaacaacacc tgccagagcg ctaccggaga atacgattgc tgcaattgct
    16621 gctactttta aaagtttcat gtaaaacccc catcggattg atttaaaagt cgaatggaaa
    16681 ttaacgttgt gtcacgcgaa tagccatttg cgactgtctc tgcactacaa ttgccgtttt
    16741 ttgagtacca tactgtgtaa tatttgcttt attaccagaa cctttctgga taatcatcgc
    16801 agtattacca taagcacctt gcgaaatact ggcatcgttg gcactgcccg cctgatcaat
    16861 atatgcaagg ttataatctc ctgtctggtc aatctttgcc cgattgctac taccttcttg
    16921 cgcaacaacc gccaaaagtt ttgagcctcc ctgccgtaac tgagcactat tattcgtccc
    16981 agcttgacca attatggctg cctgattaaa tgaagactta ctcaattcat ttaccgcgaa
    17041 gttatattct gaattagcta aatcataacc tgctgcggct gcaatcccag gcgcacccag
    17101 tattgttaac atcataaata acaatttgtt tttcatgttg tcaccctgga cctggtcgta
    17161 catttaagaa attaaatcat ttcaacttgg ttgttaacgc aacctgtatt ttgttaacgc
    17221 tgcgttacga tggaaagtat gtctgcggaa atatttttaa taactcaccc gcgcgttgta
    17281 tttttctttt tttgttcata ccaaagtatt aaaacccacg t
//
LOCUS       DAZFVO020000107        17211 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFVO020000107 DAZFVO020000000
VERSION     DAZFVO020000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.108"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(61..1101)
                     /locus_tag="ACHKNB_RS16640"
                     /old_locus_tag="pgaptmp_003328"
     CDS             complement(61..1101)
                     /locus_tag="ACHKNB_RS16640"
                     /old_locus_tag="pgaptmp_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGU4196787.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(1174..1809)
                     /locus_tag="ACHKNB_RS16645"
                     /old_locus_tag="pgaptmp_003329"
     CDS             complement(1174..1809)
                     /locus_tag="ACHKNB_RS16645"
                     /old_locus_tag="pgaptmp_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HGU4196788.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKNB_RS16650"
                     /old_locus_tag="pgaptmp_003330"
     CDS             1937..2455
                     /gene="yhbO"
                     /locus_tag="ACHKNB_RS16650"
                     /old_locus_tag="pgaptmp_003330"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HGU4196789.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2435..2878)
                     /locus_tag="ACHKNB_RS16655"
                     /old_locus_tag="pgaptmp_003331"
     CDS             complement(2435..2878)
                     /locus_tag="ACHKNB_RS16655"
                     /old_locus_tag="pgaptmp_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HGU4196790.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKNB_RS16660"
                     /old_locus_tag="pgaptmp_003332"
     CDS             2929..3231
                     /gene="yhbQ"
                     /locus_tag="ACHKNB_RS16660"
                     /old_locus_tag="pgaptmp_003332"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HGU4196791.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(3218..3721)
                     /locus_tag="ACHKNB_RS16665"
                     /old_locus_tag="pgaptmp_003333"
     CDS             complement(3218..3721)
                     /locus_tag="ACHKNB_RS16665"
                     /old_locus_tag="pgaptmp_003333"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4196792.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3715..4239)
                     /locus_tag="ACHKNB_RS16670"
                     /old_locus_tag="pgaptmp_003334"
     CDS             complement(3715..4239)
                     /locus_tag="ACHKNB_RS16670"
                     /old_locus_tag="pgaptmp_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HGU4196793.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4448..5443
                     /locus_tag="ACHKNB_RS16675"
                     /old_locus_tag="pgaptmp_003335"
     CDS             4448..5443
                     /locus_tag="ACHKNB_RS16675"
                     /old_locus_tag="pgaptmp_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGU4196794.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5452..6330
                     /locus_tag="ACHKNB_RS16680"
                     /old_locus_tag="pgaptmp_003336"
     CDS             5452..6330
                     /locus_tag="ACHKNB_RS16680"
                     /old_locus_tag="pgaptmp_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGU4196795.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGISN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6536..7543
                     /locus_tag="ACHKNB_RS16685"
                     /old_locus_tag="pgaptmp_003337"
     CDS             6536..7543
                     /locus_tag="ACHKNB_RS16685"
                     /old_locus_tag="pgaptmp_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HGU4196796.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKNB_RS16690"
                     /old_locus_tag="pgaptmp_003338"
     CDS             complement(7642..8217)
                     /gene="dolP"
                     /locus_tag="ACHKNB_RS16690"
                     /old_locus_tag="pgaptmp_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HGU4196797.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKNB_RS16695"
                     /old_locus_tag="pgaptmp_003339"
     CDS             complement(8227..8817)
                     /gene="diaA"
                     /locus_tag="ACHKNB_RS16695"
                     /old_locus_tag="pgaptmp_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HGU4196798.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            complement(8837..9232)
                     /locus_tag="ACHKNB_RS16700"
                     /old_locus_tag="pgaptmp_003340"
     CDS             complement(8837..9232)
                     /locus_tag="ACHKNB_RS16700"
                     /old_locus_tag="pgaptmp_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HGU4196799.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKNB_RS16705"
                     /old_locus_tag="pgaptmp_003341"
     CDS             complement(9190..11226)
                     /gene="lpoA"
                     /locus_tag="ACHKNB_RS16705"
                     /old_locus_tag="pgaptmp_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HGU4196800.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKNB_RS16710"
                     /old_locus_tag="pgaptmp_003342"
     CDS             11290..12150
                     /gene="rsmI"
                     /locus_tag="ACHKNB_RS16710"
                     /old_locus_tag="pgaptmp_003342"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGU4196801.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQKGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            complement(12193..13284)
                     /locus_tag="ACHKNB_RS16715"
                     /old_locus_tag="pgaptmp_003343"
     CDS             complement(12193..13284)
                     /locus_tag="ACHKNB_RS16715"
                     /old_locus_tag="pgaptmp_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196802.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(13295..15811)
                     /locus_tag="ACHKNB_RS16720"
                     /old_locus_tag="pgaptmp_003344"
     CDS             complement(13295..15811)
                     /locus_tag="ACHKNB_RS16720"
                     /old_locus_tag="pgaptmp_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4196803.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            complement(15841..16536)
                     /locus_tag="ACHKNB_RS16725"
                     /old_locus_tag="pgaptmp_003345"
     CDS             complement(15841..16536)
                     /locus_tag="ACHKNB_RS16725"
                     /old_locus_tag="pgaptmp_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4196804.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            complement(16616..17200)
                     /locus_tag="ACHKNB_RS16730"
                     /old_locus_tag="pgaptmp_003346"
     CDS             complement(16616..17200)
                     /locus_tag="ACHKNB_RS16730"
                     /old_locus_tag="pgaptmp_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196805.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
BASE COUNT         4081 a         4561 c         4352 g         4217 t
ORIGIN      
        1 cgatgcaaac cgtaggtcgg ataaggcgtt tacgccgcat ccgacatgtt ttccctcaaa
       61 tcagaacaac agcgccagcc cgccgacaat cacaccagac actgccacca tcgcccctgt
      121 cagccataag gctttcgctg ggaacgcttt gcgcagcata atcagtgacg gcaaactcac
      181 cgccgggagc gtcatcaaca atgccagcgc cggagcagtt cccatacctg ccagcatcat
      241 cgtttgtaca atcggaattt ctgctgccgt gggaatgaca aacaagcatc ctgctaccgc
      301 catcgccatc acccacatca ggctgttatc gacagcacca tcggcatggg ggaataacca
      361 gacgcgagcg gcacccaaca ccagtactgc aaggatgtaa accgggatcg tactccagaa
      421 aagcgtccat agcgccctgc cccagcggct aaaaaaaccg ccctgtgctt ccggtatgtc
      481 aatttcgacc ggtgcctgcg tttgcggtgt ttcacgcacc catttttgca ccagcgtcgc
      541 aatcagcaac accatcacca gcccggccac cagacgaatc gccgcaaaac cccagccgag
      601 gacaaacccc ataaacacca gcgtcgccgg gtttaacacc ggattgccca tccagaatgc
      661 cagcgcaccg cccatcgaca cctgttggcg acgcattccc gccgcgaccg gagccgcaca
      721 gcaggtacac atcatgcccg gcagcgaaaa cagcgttccc agcagcgtgc cgcgaaagcg
      781 cgattgccca agcgtacgca acaaccagtc acgcgggatc agcacctgaa tcaacgaacc
      841 gaggatcacc cccagcactg ccgctttcca taccgcgagg aaatagatca tcgcgtaatc
      901 caacgccgcc tgccacggat tagcatccgc ctgcgcaagg atagatttac cgatactgtg
      961 ggtttcggca gcagtaaacg ctttgccgta gtaaggttgc catttcacat accagagacc
     1021 ggcaatgaca acgagaaaga aaagcgcggg tttccaccac tgaatgggcg ttgccgcctg
     1081 agatgaagac tgaccagtca tagcattccc caggaataag ttatgataat tgagcgcgtg
     1141 aatattacgc tcactatcaa ttcttgggaa taattattca gctcttttgc gtaattctga
     1201 agagcttaaa atcgtcacac cttcatgccc gggccgcatc gcctctgcca gcatgacccg
     1261 cgccacgtct ctcgcatcaa tggatttcca gttaccgggt aataaacgga acagcggcgc
     1321 aaaaagcgtt tcgttcatcc gctgtttgct acgatcgccc agtaacatcg acgggcgagc
     1381 aatggtcaat ttcggccagt tctgggcgat taatgcttct tccatctccc ctttgacgcg
     1441 gttatagaaa aacggcgagt gggcattggc acccatagca ctgaccacca gcatatgctg
     1501 cgcgcccagt cgccgcccgg ttaatgcggt atccactacc agcgtgtaat cggcatgaat
     1561 aaacgcctct ttgctccccg cttctcgccg cgtggtgccg agacaacaaa acacaatgtc
     1621 gatgggatcg gtgacctgcg ccagcgcgtc agtcagttgc ggatcatggg gattaaacac
     1681 cccgggcata tcgcccaacg gacgtcgcgt cggcgcggct atggcgttaa ctttcggttc
     1741 gttaatcaac atccgcagca ggtgaccgcc caccagcccc gttgcgcctg taatcagtac
     1801 ctgactcatc ttcgctcctt tacagaattg tctgccttgc gctccacggc tcatgcacca
     1861 ggcttaatag accatgaggt aattatcccc gattgtggga aattcgcctc atccaatgca
     1921 acaacgcgga ggaagcatga gtaagaaaat tgccgtttta atcactgatg aatttgaaga
     1981 ttcagaattt acttcacccg cagacgagtt ccgtaaagcc ggacacgaag tgattaccat
     2041 tgaaaaacaa gcgggtaaaa cggtgaaagg caaaaaagga gaagccagcg tgaccatcga
     2101 taaatccatc gatgaagtga cgccagcgga gtttgatgcc ctgctgctac cgggcggcca
     2161 ttcaccggat tatctgcgtg gcgacaaccg ttttgtcacc tttacccgtg attttgtgaa
     2221 tagcggcaaa cctgttttcg ccatctgtca cggtccgcag ctgctgatca gcgccgatgt
     2281 gattcggggg cgcaaactga ccgcggttaa accgatcatt attgatgtta aaaacgcggg
     2341 cgcggaattt tacgatcagg aagtcgtggt cgataaagat cagctggtta ccagccggac
     2401 accggacgat ctgccagcgt ttaaccgcga ggcgttacgc ctgctcggtg cctgagccgc
     2461 gtagccaaat cattttttta ccaaagccca gcgtgttgtc ggtgaatttg atttcatcga
     2521 gccggatttc ccacaccggg gccgacagca ttctggcaac cggaaagcga cgattgtacg
     2581 ccttgcgcgc gaggtcgctt tcctcacctt ccagcctgcg gatctcacct ttaaactgca
     2641 cgccgcgaat taacgctacc gttttcggct gaccgtttac cgttccggca accgcagcct
     2701 gcggcccact catctgcgcg tggcgcgttt tttcttccgt caaaatgtag aaggcgactt
     2761 tctgcgcatc aaacagataa aaagcattag cgcaccagag ttccccttcc tgctgcacac
     2821 accaggtgac aacatgttgt tttgccaacc aacggctgat ggtgatgagt gtttccattg
     2881 ctgttctctt ttatactgtg ggcctgacct taacatgcga cgcagacgat gacaccctgg
     2941 tttctttact tgatccgtac cgccgacaat aagctttata ccgggatcac cacggatgtc
     3001 gaacgccgct atcagcagca ccaaagcggc aaaggggcga aagcactgcg cgggaaagga
     3061 gaactgacgc tggcgttttc tgcgccagtg ggcgatcgtt cgctggcgtt acgggcggaa
     3121 tatcgcgtta agcaactgac caaacggcaa aaagagcgtc tggtagcgga aagcgcaggg
     3181 tttgcagagc tgttaagcag tctgcaaacc ccggagatta aaagcgattg aaatgctcgt
     3241 gatactcaac cagaccagtt acgccattca acgcgtcatc cgctaaacga tgtacctgga
     3301 aggcgctttc agtgcctggc cagcggcaac gcaggtcgtg atgtgccgcc agttcaaagc
     3361 caaaacggct gtacagcgcc ggatcgccca gcgtcaccac tgcggcatag ccgaactcat
     3421 taagcgaatc gagtccttca tagaccagtt ggcgtgccag cccctgtcca cggtattttt
     3481 catcaaccgc caacggtgcc atgccgaccc attgcaggtc ttcgccctgc acatcaaccg
     3541 gactaaatgc cacatagcca atgacctgac cttcgtcatc tgtcgccacc agccccagcg
     3601 tcagaaagcc atcttcacgc agatcgtgaa ccagcttcgc ttccgcatca ctttcgaatg
     3661 agcgacgtag cagggcatca atacccggcg catcaatggg aatttctact cgaattagca
     3721 tggttcacct accgatgtct gtttggtttc aggcgcggtt ttcattcccg cctcaacaaa
     3781 atccgccagt tgcagcagca tcatgcgtaa ggctttcggc atttgctcca gttcaatggc
     3841 gtccatcagg tttttcacat acagccccag ctccgtatcg ccttcaatca ccagccgacg
     3901 ctggaagaag agcgtatccg gatcttgttt acgcgccgca atcatcagca gatcgctggc
     3961 gtcggcacta aaactcacat cagcttgcgc gttctggctg accaccagtt tgccattcac
     4021 caccgaggta aaccattgca ggtcaatatc acgcacatga atacttaacc agcggccttc
     4081 aagaaactcc agctcgccat catccagcgc ctgacggaat tgccagctta agacctgctc
     4141 aagaacctgg cgttttagcg caaatggcgt cagttttacc ggtacactca acagagatgg
     4201 ccccaaatgc acaatacggg aacgcagttt atccaacacg agttttactc cctgtttcaa
     4261 caatcatcct attttgccat atcagaaaaa taacatagcg gtataaatca acaattccat
     4321 atgaaattgc tgctaccacc aatacaactt taactgcctt aaatcaaaaa ttgtcgcagc
     4381 aaggttaact aaaatcccag ttcgttaaca tttttgcgtt ttgatagcgc aaccttcagg
     4441 aaaaattatg gagctgctct gccctgccgg aaatctcccg gcgcttaagg cggccatcga
     4501 aaacggcgca gatgctgttt atatcgggct aaaagatgat accaatgccc gtcacttcgc
     4561 cggccttaac tttaccgaga aaaaattgca ggaagcggtg agttttgtcc atcaacatcg
     4621 ccgcaaactt cacatcgcga ttaacacttt tgcgcatccg gacggttacg cccgttggca
     4681 gcgtgccgtg gatatggcgg cgcagctggg tgccgacgcg ctgatcctcg ccgacctcgc
     4741 catgctagag tacgccgccg agcgttaccc gcatattgag cgtcatgtgt cagtgcaggc
     4801 ttcggcgacc aatgaagagg cgattaactt ttatcatcgc cattttgacg ttgctcgcgt
     4861 ggtgctgccg cgcgtgttgt cgattcatca ggtgaaacaa ctggcacggg tcacacctgt
     4921 accactggaa gtctttgctt tcggcagcct gtgcattatg tcggaaggtc gttgctatct
     4981 gtcgtcgtat ctgacgggtg agtcgcccaa caccgtaggc gcgtgttctc cggcccgttt
     5041 cgtgcgctgg caacaaacgc cgcaggggct ggaatcccgc ctgaacgaag tgctgatcga
     5101 ccgttatcag gacggcgaaa acgcaggtta tccgacgcta tgtaaagggc gttatctggt
     5161 ggacggcgag cgctatcacg cgctggaaga accaaccagt ctcaataccc tggaactgct
     5221 gccggagtta atggcggcga atattgcttc ggtgaaaatt gaaggccgcc agcgtagccc
     5281 ggcgtatgtc agccaggtgg cgaaagtctg gcgtcaggct atcgaccgtt gtaaggccga
     5341 tccgcaaaac ttcataccgc aaagcgcgtg gatggagacg ctcgggtcga tgtccgaagg
     5401 cacgcagacc acccttggcg cgtatcaccg taaatggcag tgagaagagc aatgaaatat
     5461 tccttagggc cagtgctgtg gtactggcca aaagagacgc tggaagattt ttatcagcag
     5521 gccgccacca gcagcgccga cgtgatttat cttggtgaag cggtatgcag caagcgccgg
     5581 gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc tcgccggaag tggcaagcag
     5641 attgtgctct ccacgctggc gctggtgcag gcatcctctg aactgggcga actgaaacgc
     5701 tatgttgaga acggtgagtt tctgattgaa gccagcgatc tcggcgtggt gaatatgtgc
     5761 gctgaacgca aactgccgtt cgtcgccggg cacgcgctga actgctacaa cgcggtgacg
     5821 ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta tgccggtgga gctttcccgc
     5881 gactggctgg tgaatctgct taatcagtgc gatgagctgg gcattagcaa ccagtttgaa
     5941 gtggaagtcc tgagctacgg tcatctaccg ctggcctact ccgcccgctg ctttaccgcg
     6001 cgttcggaag accgcccgaa agatgagtgt gaaacctgct gcattaagta tccgaacggg
     6061 cgcaacgtgc tgtcgcagga aaaccaacaa gtgtttgtac tcaatggcat tcagaccatg
     6121 agcggctacg tttacaacct cggtaacgag ctggcatcca tgcaggggct ggttgatgtg
     6181 gtccgcctgt caccgcaggg tactgacact ttcgcgatgc tcgacgcctt ccgcgctaat
     6241 gaaaatggcg ctgcgccact accgttgacg gcaaacagcg actgtaacgg ctactggcgg
     6301 cggctggccg ggctggagct gcaagcctaa gtaaatatgc tcgtcatact tcaagttgca
     6361 tgtgctgcat ctgcattcgt tcaccccagt cacttactta tgtaagctcc tggggattca
     6421 ctctcttgtc gccttcctgc aactcgaatt atttagagca cagctcactt tgttaacaac
     6481 tttaactact ctttaatgca gtattaaaga ttaatcggta acaaagtgag ctgttatgac
     6541 tgataaaacc attgcgtttt cgctactcga tctggccccc attcccgaag gttcttcagc
     6601 gcgagaagca ttctcccact ctctcgatct cgcccgtctg gctgaaaagc gcggctatca
     6661 tcgctactgg ctggcagaac accacaatat gactggcatt gccagtgctg ccacgtcggt
     6721 gttgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg gcggcgtgat
     6781 gttgcctaac cactcaccgt tggtcattgc cgaacagttc ggcacgctca atacactcta
     6841 tccggggcga atcgatttgg ggctgggtcg tgcgccaggt agtgaccaac ggacaatgat
     6901 ggcgctgcgt cgccatatga gcggcgatat tgataatttc ccccgcgatg tcgcggagct
     6961 ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac caggctatgg
     7021 cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agcgcgcaac tggcggcgca
     7081 gcttggtctg ccgttcgcgt ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct
     7141 gcatctttat cgcagcaact tcaaaccgtc ggcacgactg gaaaaaccat acgcgatggt
     7201 gtgcatcaat attatcgccg ccgatagcaa ccgcgacgct gagtttctgt ttacctcaat
     7261 gcagcaagcc tttgtgaagt tgcgccgtgg cgaaaccggg caactgccgc caccgattca
     7321 aaatatggat cagttctggt cgccgtccga acagtatggt gtgcagcagg cgctgagtat
     7381 gtcgttggta ggtgataaag cgaaagtgcg tcatggcttg cagtcgatcc tgcgcgaaac
     7441 cgacgccgat gagattatgg tcaacgggca gattttcgac caccaggcgc ggctgcattc
     7501 gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct taacgcggga
     7561 agccgtatcc gagctggcaa cgctgtccta caaggcctga taagacgcgg caagcgtcgc
     7621 atcaggcatt acaaggggct gctatttaat aaacgtaaac gctgtggtta cccgctttac
     7681 gccgctcacc cggctggcaa tatctgccgc cgctttcgct tcacgttcag tcaccagccc
     7741 catcaggaac acttcaccgt tttcggtggt cactttcacg ttggacgatt tcaccaggtc
     7801 gctggttaag agctgcgaac gcactttggt ggtgatccac gtatcgttag acgcttcgcc
     7861 cagaccaatc ggctggccct gacgaatctc gttatacact tcgttggcac cgtctacgcc
     7921 catagcaatc tgtttggcgc gagccgaaag ttcagcgttt ggtgactgcc caaccagcag
     7981 cactttgccc tgatacgccg tgacattaat acgggcttct ttcttaatct gttcgtcttt
     8041 cgacaatgcg ctgttcacgc gcacttccag ggtaccatcg tccacctggg tgccgacact
     8101 gcgtgggtca gttgcggctt tggtacccac agcagcggta cccactacgg cagcggcaac
     8161 acaaccttgc aacagcagcg cggaaataag gactgcgatt ggcgataatg ccttcatgta
     8221 ttctccttaa acatcctggt gagggaaaag cgtgttatcg atcagatcgc acaggcaatt
     8281 taccgtcagc atatgcattt cctgaatgcg agcactacga tgcgaaggaa tgcggatctc
     8341 cacatcttgt ggccctaaca aacctgcaag ttcgccgccg tcatagccgg tcaatgccac
     8401 aatagtcata tcacgcgtaa cggcagcttc taccgctttt acaatatcgc ggctgttgcc
     8461 acgggtggaa atggctaaca atacatctcc cgcatgaccc agcgcccgca cctgttttgc
     8521 atacacttca tcatgtaagc gatcgttggc aatcgccgtt aagacaacat tatcagtatt
     8581 tagtgcaatg gcaggtaagc tgggccgctc cgtttcgaaa cggttgatca tgctggcagc
     8641 aaaatgctgt gcattggcag cggaagttcc attaccacaa cagaggattt tgttgccatt
     8701 gagcagagac tgaaccagcg tcatggctgc acgggagatg gcatccggaa gcgcctctgc
     8761 cgccgcaatt tgagtttgaa tgctttcagt gaagcaagct ttaattcttt cttgcacgct
     8821 aatccttaaa ccttaattat gagtggtcat taaaggcatc cttaatccac tcaacctcat
     8881 tcccggtgaa ggctaccaca tcgaaccggc aatccacagt atcaaaactc ccattatgac
     8941 gcgcgagcca caagcgggca gtctgtaata atttgtgttg tttgctgcgg gtcacactgg
     9001 ctgccgcgcc gccataaagc gcagagcggc ggtaacgcac ctcgacaaaa acggtggtcc
     9061 ggccttcacg cattatcaga tcgatctcgc cgccacgctc gttcacgtta gcggcgacaa
     9121 atcgcagtcc tttgccttcc agccagcgac gcgcttgtac ttcccacgca tcgccggtct
     9181 gtttggtggt taactggcgg ggactacctg accttgttgg tactgtagcc atgataactt
     9241 cctgttaatc acgcaatccg ggtttgccgt caggcttccg gtattaccgt tgatttcaaa
     9301 accctgaacc tggcgcattt gtgagaaatg atttgccagc gaccaggcat cgacgcccat
     9361 cgcatacatg cgagccagtg aataatcgtt attcaccgcg ctgagtgcct gctgcattaa
     9421 cggtagatta ccgcctgcca gcatcgggat ttcgctgtac tgcaagcctt ccatctccag
     9481 tcggaaatcc gggccagcgg tcccttgcgc actgcgggag ctggcgtaca gcgttgcacc
     9541 gctctggcta ccgttacgca tggcgatcat cggtttgata aaagcgattt cacccggcgt
     9601 tgccacaatg tacaccgcat cgacacgacc gccattattg gtgaactggt catcggttgg
     9661 cgtggtttgc agcgttggat tgttggtcgt catgccggag tcggttgtcg ctctgggagt
     9721 aatcggggta cccgttaaag cgataccaga accgccgtta acacccgcgc gtaattcgct
     9781 ggtggaacca aatttttgtt gcagaacggt gccgccgccc agtttctgcc actcctgcgc
     9841 aaacgcattg gctacgcgat cgcccaatga actgcgtggg atcagcacca gcggcgcttg
     9901 tttaccctgg tcacgaatat gacgcgctgc atcgcgcgct tcgtcttccg gtgaaagcgc
     9961 gaagtaacaa atattgacgc ggttttcgat attctccggc tggttcagtg ccagcacgtt
    10021 cagcggggtg ttgctcttaa gcaactcttc aacgttattt ttcagcaacg gaccaaccac
    10081 aatactcgcg ccatcctgct gaacctggct taagatctgg ctaagtggtt gtgatgaggt
    10141 atcgtagatt ttcagttctg cggatggatt cgctggcgcg cttaccgctg tggtggttgc
    10201 tgccggggtg cttacaggcg ctggctgtgc cgcaggctgt tcaccggtca gatcgctaac
    10261 cgaggcttgt gccgggctgg caacgccatc cacggtttgc ggctgaggtt gttcagctac
    10321 gtctgcggca ggtgcggcag ctacctgagc cgccactggc tgagtgccga tatttttcgc
    10381 cgcttcaaag ccttgctgaa tagtgcgacc aaataccgct gcctggccat tcagcggcaa
    10441 cagcagggcg attttgttgg tcgaggctgg tttaaacgct tttacgttaa ccaactgcgt
    10501 tggcagcatt ttcgcgcccg gattgttcgg ataacgtttt tgccagtcgg cgatcccggc
    10561 tttcatcatg tcgggatcgt tacggttatc aaaccagacg cgctgcagat ccagccagcc
    10621 ttgcagaata ttttcgtcgg cgttgatcac cagcgtattc gcctgttcct gagtcatgga
    10681 ggagagcgcc tgccaggtgg catcaatatt ctgctgtttt tctttcgcgc cgagcagcgg
    10741 ttcctgagca attaacgcgc gcagtaaatc aatggaagga cgcccctggc tggcatcgat
    10801 ttttgcctgc cagtaacgcg cttgctggtt ttgttctaaa tcggcaggtg tgattttcgc
    10861 cagcaagttt tgcgcgccag caaaatcttt ctgcgccagt ttaatctcta ccgccagcag
    10921 tgttttctcg cgacgctgag aatcgttcag ttcttgcggt agttggttaa acaactcaac
    10981 cgcctgcccg gttttacctt ctttcaccag tgcacgaatg gcgagtaatt gccagttgat
    11041 cctggtatca tcagagcttt gctgcatctg ctgaagataa aaggcagaat cagcctgcgc
    11101 cgtgccctgc atataagcag tggactgatc gggagtatgg gtgccacaac cggcgaaaat
    11161 cagggctgcc agaacaacag gcagacaacg cgcggctttc aaacgagaaa atgttgaggg
    11221 taccataatg tatccagtga tattttttta cgcaatgctc aatattaaat cggcaatacg
    11281 gacgacacaa tgaaacaaca ccaatcggcg gataattctc agggccagct ttacattgta
    11341 ccgacgccaa tcggcaatct ggcggatatc acccagcgtg cgttagaggt attacaggcc
    11401 gttgatctga ttgccgccga ggatactcgt cacaccggtt tattgctgca acattttggg
    11461 attaatgccc ggttgtttgc gctgcacgac cataacgaac aacaaaaagc cgaaacgctg
    11521 ctggcgaagc tgcaaaaagg gcagaacatt gcgctggttt ccgatgccgg aacgccgcta
    11581 attaacgatc ctggctacca tctggtgcgt acctgccgtg aagcggggat ttgcgtggtg
    11641 ccgctacccg ggccgtgtgc tgctatcact gcgttaagcg ccgcgggttt accctctgac
    11701 cgtttctgtt acgaaggctt tttacctgcc aaatcaaaag gccgccgtga tgcgctaaaa
    11761 gccattgaag cggagccgcg cacgctgatt ttttatgaat ccacccaccg tctgttagat
    11821 agcctggaag atatcgttgc ggtattaggc gaatcccgct acgtggttct ggcgcgtgag
    11881 ctgaccaaaa cctgggaaac cattcacggc gcgcccgttg gcgagctgct ggcgtgggta
    11941 aaagaagatg aaaaccgtcg caaaggcgaa atggtgctga ttatcgaagg tcataaagca
    12001 caggaagaag acttacccgc cgatgccctg cgcacgctgg cgctgctaca ggcagaactg
    12061 ccgttgaaaa aagcggcggc gctggcggca gaaattcacg gcgtgaagaa aaatgcgctg
    12121 tataagtatg cgctggagca gcaggggtaa gagtgccgaa gcgttgcgtt aatcaaaacg
    12181 caacgtcttc ttttaccgca cgatgacggt aatggtggcg gtgccctgaa attttcctgt
    12241 ttccagctca ccaccgacca gattaactgg ccaggcgcgc atgttgatgg tgccatgccc
    12301 ggaaggatca acgggaagga taccgttctg aaaaggaata tttgcaactc cgccgccgag
    12361 attttcaata cgaataccga catcgggcgc attgtctgaa cttcttcgtc tggcgaccag
    12421 gttgtactgg tctactaccc cagttgtacc gtcgatgcgc atttgcaacg agtttttaat
    12481 ctttgaagta tcaccacagt cataagtgat atcaaagtct actggagtaa aaccgtctgg
    12541 catggcattg cgggtggtaa acttctgacc attgatgaat ccaaaattaa ccttaataac
    12601 atcgccctga ttaattttgc acgactgtgg ggcggtgaga tttccctgaa tcgataactt
    12661 gactaaatca tggaagtcac ctgtagacgg gcttcccatt ccatcgaccc atgcgccttt
    12721 aatgacggca atatccgttt tcggaatggt catcgagccg agaaatggct tggtgacata
    12781 cagggtaaag gtggtatttg caccaatatt aacgggtcca taacgagacg tgtcaccctg
    12841 gcaaacaccc gtttgggttg cagcagaata tacggtgttg ctttttatct gtgtgaatcg
    12901 ggtgttaaca gaaacggtct ggtcagtcac gagtcctgga atgtcattcg cctgtaatgt
    12961 ggttttaata tccagactat cattgagttt gtaatatcct gcttgatgtc cagtggtggt
    13021 aagtacaggg ctgaccatat agacaagttt aactgcctta cccgcagagc aggtacaggc
    13081 gtattgccag ttagtgctac ttagcgtggc agaggtaaac tcccgtcctg tcacgttatc
    13141 tgtctggttc accgtaacat catcaaccac actgctgtaa tttgtcgcac cgctagtgct
    13201 gtcagctcca caccctcctg aggaggcata agcgcaggat gtggatatca acaaaagtcc
    13261 tgttataaga ggcgctcttt tcattttttt atccttaatg gcactgcgcc gtgccttgta
    13321 atacagaggt tgttggttct gaatccggtg tactaaaagc aaactgacat tgttgatctg
    13381 cctgatttcc ccagcgtaca gtccagcttt gtgatttact cgatatccca gagagatata
    13441 gtatgccgcc ctcatcgacg atactttgtt gccccgtctc atcgttgctg gcaagagcgc
    13501 caaagggcaa cggtttaccg ttgcgatcgc tgactgtaac aagtacgcga taaccgatat
    13561 tggcgttgaa acgcgccgct accattgcac ctctgttggg caccacaaac tgtgttgttt
    13621 gctcaagatc gacgttatcg ggcagctgcg tagtatctac cgagagacgg ttttcctggt
    13681 aagtcgtgag ataaggaacc actgcatagc caaagggatc agtagcaatc cccggatagt
    13741 tttgtatcct cacgccagaa gccccattag catcgataag cgcaaaagcg ttgcccagat
    13801 attgcgagag cgtcacgcca tgaggatgga taaccacgcc gccggtaacg ccgtaattgt
    13861 attggctgct gtcattaccg tagctgtatc cggcactgaa ggtgccataa ggtgaacggt
    13921 aactggcgtt caggctactg ttatggttgt tatcgtcatc cagactttgt tccaggctat
    13981 agctcaggcg accatcatcc agcagtgagc catcaagacg catttcatgt tgggttgggc
    14041 gatctttctg gctggtcatc tgataggaaa cccggctacg cggtaaccag cgttctaacg
    14101 gaatactgag attcaaagag agtgcgcggt cattattttg atccgtgtag cgactgtatt
    14161 gataattcag gctgtaacca attccccatt gctgaccgga aaccccaaca gagatattcc
    14221 tgtttttctc gttattgccc caatagtctt gctgcgaacc ggaggcatac agacttaccc
    14281 catcaaatat tgtctggctg atgttgaatt gaatttcact tttttggcga ctgttatagt
    14341 tccaattacg ggtattggct tcattaaaac taaaatagcc atcgttggta tagcgatagt
    14401 agctgacagc gatattggtg ttggtttccg ggatatcttt gatgtactgc gtacgccatt
    14461 gatagccatg aaaagagtgc tggttatcga attgggtgtc agctctgttg atatccatcg
    14521 acaacgcgcc cagtgcgcca agtgtgccgc cgataccgat ccccagcgca taataatctt
    14581 cagaaccgag caggccgcca tacagcgtaa aagtattatt caaaccatat attgccgtgg
    14641 cttcagcaaa ttcgggttcc ttgctatcac tgtttgcatc agcgcgatag cgtccagcgg
    14701 tcgcgctata ttttagatgc ccaggtcgct gcatcatggg taatgaagaa taaggctgga
    14761 taaagcgacg ttgcgtaccg tcactttctt caatcgtgac ttctaaatcg ccgctgttgg
    14821 aagaggggta gagatcgtta atttcaaagg cacccgctgg cacgttgctt tgatagatca
    14881 cataaccatt ttgcctgata gtcacgattg cactactgtt tgcgataccg cgtaccgttg
    14941 gggcaaatcc gcgctggctg tttggcaaca tattatcgtc ggaagcgagt tgcacgccag
    15001 taaaggtgta gctggaaaaa atactgccgc tggtggcgct ttctcccaga agcaactgag
    15061 acttcaacgc cttgatatca cgttgtaaat aactactgat agtgttccag cttgacgcct
    15121 gatcgttgcg tgtccacgta gaatagttac gtaatcgcca ggggccaaaa ttagcaccat
    15181 tttgcatatt caggtactgt cgttggctac gattgccctg gcggtaacgg ttatcagaac
    15241 ctgtaaacga gtagttggta aacagcgttg gtataccatc gtcccaacga gaaggggaga
    15301 cgtaacctct tgcatcacgg tacagtgcaa tttggggaat gctcaaatta agtcgctgat
    15361 gattgaaatc aaattcagca gctgtaccgg gaatgatttg ttcaagcgag ttgatcacgc
    15421 tatcgtcatc tttttcagcc agcgccggga tttcatccac cttaataccc agctcacgta
    15481 gttgttctac cgtaaactgt ggctgcagaa gttgctctgc attggcggta aatgtaattt
    15541 ttttctgtga aaccttcttt ttattcagcc agatatcaac ctgataagtt cctggtagtt
    15601 gtgcgtattt ttttgaaaaa cgtgacagat cagttgtttg cataccactt tttgtagttt
    15661 ccagcataat ggggtcgaaa taatactctt cggcgttagc ggtagtgcaa agcatgccgc
    15721 tgccagtgag caccatcggc aaacagcgtt tgatgatgag cgccaactgt ttcggtgtgc
    15781 gtttatgtcc ctggtggtgt cgttgtggca ttccctgatg acctgtgttt tttatatttg
    15841 ttattgcagt ggacgagttt ctgtcccgga actgccgcca taatcattga ttgcagccca
    15901 tttcacgttc tttccgccag atgcaggaag tggtaactgt ttatgttctc cgggcgcgat
    15961 atattcaatt ttgcttgtga gatcggtttt atcgactgcc agtccggcaa atacgacgta
    16021 atattcagtt gggttatata tattgagtga atggtcacta cgtttaaatt ccagtgatgt
    16081 ccacgcatcg tttacccgtc ccttcaggtg tgcggggcga taaaacattt tgatccgtgt
    16141 tttaaatacc agcgtcaact cgttagcatt aacatcatct gattttttct ttgctggaat
    16201 ggcacgaaca ttaatgtaga acagcgactc tttattttca ggcaggttat cagctgtttt
    16261 aataattcgc agtgatgagt catcgccagc ttcaagacga aatagcggtg gggttataat
    16321 aaatggggca cggcttgtac catctatatt atctacccat gattgcagta aataaggcgt
    16381 ttctgcgcct ttgtttgcga caggtaacgc agcctctttg cggctggcat catagatcac
    16441 tcgcgttcgc tgtaaaacga taccccctgc aagcgcctgg ccgatacaga agctacacaa
    16501 caaggttatt atcaccgcga atgttcgttt tgacatagtt atttttcaac ctgatacaac
    16561 gagaaagagg aaggaaaagg gcgttatctg aaaggtcaga taacgccgta acgtattaac
    16621 ggtaatccag cgttacgtta acgacagcgt ctgcttcccc tggggtgatt tctgatgctt
    16681 caacggttga tacgtagtaa gcgatgaggt caacattgac aataccattg gagtccgctg
    16741 ggatctgata aaccgtgtta tcattcccca tagcattcag tggcataagg tttccgcttt
    16801 ccgcatcgtc aatacggata cccacacctg ttgccgctgt gccagcaaaa gagcccactt
    16861 ttgccagatt agggaagtta gggacggtgc cattatcccg cgtttccagc cataaacgga
    16921 aagacgcttc aacgttgccg ttatcgtgct cccacccagt cactgggcag ttaactaaag
    16981 gaatagtgaa tggaattttt gggctacgat cgccaacaga acgaaactgt tcggcattat
    17041 agtgaccaag agcaacttca attttcttat cagagtcatt gatttcacaa gcggatttta
    17101 aaatctcgcc atcaaaaact atttcaccat cggttgcagc ggcattacct gcgaaaagcg
    17161 caagtaagcc cacaatagct gtttttgtaa ctttattcat ttataacctt a
//
LOCUS       DAZFVO020000108        17137 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFVO020000108 DAZFVO020000000
VERSION     DAZFVO020000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.109"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            82..684
                     /gene="ais"
                     /locus_tag="ACHKNB_RS16735"
                     /old_locus_tag="pgaptmp_003347"
     CDS             82..684
                     /gene="ais"
                     /locus_tag="ACHKNB_RS16735"
                     /old_locus_tag="pgaptmp_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HGU4196806.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(723..1148)
                     /gene="nudI"
                     /locus_tag="ACHKNB_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /pseudo
     CDS             complement(723..1148)
                     /gene="nudI"
                     /locus_tag="ACHKNB_RS16740"
                     /old_locus_tag="pgaptmp_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
     gene            1427..1969
                     /gene="yfaZ"
                     /locus_tag="ACHKNB_RS16745"
                     /old_locus_tag="pgaptmp_003349"
     CDS             1427..1969
                     /gene="yfaZ"
                     /locus_tag="ACHKNB_RS16745"
                     /old_locus_tag="pgaptmp_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HGU4196807.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2069..3271
                     /gene="yfaY"
                     /locus_tag="ACHKNB_RS16750"
                     /old_locus_tag="pgaptmp_003350"
     CDS             2069..3271
                     /gene="yfaY"
                     /locus_tag="ACHKNB_RS16750"
                     /old_locus_tag="pgaptmp_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HGU4196808.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3335..3796
                     /locus_tag="ACHKNB_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /pseudo
     CDS             3335..3796
                     /locus_tag="ACHKNB_RS16755"
                     /old_locus_tag="pgaptmp_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3837..4739)
                     /gene="rpnE"
                     /locus_tag="ACHKNB_RS16760"
                     /old_locus_tag="pgaptmp_003352"
     CDS             complement(3837..4739)
                     /gene="rpnE"
                     /locus_tag="ACHKNB_RS16760"
                     /old_locus_tag="pgaptmp_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HGU4196809.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRQLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4933..6123)
                     /gene="glpC"
                     /locus_tag="ACHKNB_RS16765"
                     /old_locus_tag="pgaptmp_003353"
     CDS             complement(4933..6123)
                     /gene="glpC"
                     /locus_tag="ACHKNB_RS16765"
                     /old_locus_tag="pgaptmp_003353"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HGU4196810.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6120..7379)
                     /gene="glpB"
                     /locus_tag="ACHKNB_RS16770"
                     /old_locus_tag="pgaptmp_003354"
     CDS             complement(6120..7379)
                     /gene="glpB"
                     /locus_tag="ACHKNB_RS16770"
                     /old_locus_tag="pgaptmp_003354"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HGU4196811.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALITVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7369..8997)
                     /gene="glpA"
                     /locus_tag="ACHKNB_RS16775"
                     /old_locus_tag="pgaptmp_003355"
     CDS             complement(7369..8997)
                     /gene="glpA"
                     /locus_tag="ACHKNB_RS16775"
                     /old_locus_tag="pgaptmp_003355"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HGU4196812.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9270..10628
                     /gene="glpT"
                     /locus_tag="ACHKNB_RS16780"
                     /old_locus_tag="pgaptmp_003356"
     CDS             9270..10628
                     /gene="glpT"
                     /locus_tag="ACHKNB_RS16780"
                     /old_locus_tag="pgaptmp_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HGU4196813.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10633..11709
                     /gene="glpQ"
                     /locus_tag="ACHKNB_RS16785"
                     /old_locus_tag="pgaptmp_003357"
     CDS             10633..11709
                     /gene="glpQ"
                     /locus_tag="ACHKNB_RS16785"
                     /old_locus_tag="pgaptmp_003357"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4196814.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11751..11990)
                     /locus_tag="ACHKNB_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /pseudo
     CDS             complement(11751..11990)
                     /locus_tag="ACHKNB_RS16790"
                     /old_locus_tag="pgaptmp_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11988..12092
                     /locus_tag="ACHKNB_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /pseudo
     CDS             11988..12092
                     /locus_tag="ACHKNB_RS16795"
                     /old_locus_tag="pgaptmp_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12172..12822
                     /gene="inaA"
                     /locus_tag="ACHKNB_RS16800"
                     /old_locus_tag="pgaptmp_003360"
     CDS             12172..12822
                     /gene="inaA"
                     /locus_tag="ACHKNB_RS16800"
                     /old_locus_tag="pgaptmp_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HGU4196815.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12876..13130)
                     /gene="yfaE"
                     /locus_tag="ACHKNB_RS16805"
                     /old_locus_tag="pgaptmp_003361"
     CDS             complement(12876..13130)
                     /gene="yfaE"
                     /locus_tag="ACHKNB_RS16805"
                     /old_locus_tag="pgaptmp_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HGU4196816.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13130..14260)
                     /gene="nrdB"
                     /locus_tag="ACHKNB_RS16810"
                     /old_locus_tag="pgaptmp_003362"
     CDS             complement(13130..14260)
                     /gene="nrdB"
                     /locus_tag="ACHKNB_RS16810"
                     /old_locus_tag="pgaptmp_003362"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGU4196817.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14407..16692)
                     /gene="nrdA"
                     /locus_tag="ACHKNB_RS16815"
                     /old_locus_tag="pgaptmp_003363"
     CDS             complement(14407..16692)
                     /gene="nrdA"
                     /locus_tag="ACHKNB_RS16815"
                     /old_locus_tag="pgaptmp_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGU4196818.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16820..16963
                     /gene="ypaB"
                     /locus_tag="ACHKNB_RS16820"
                     /old_locus_tag="pgaptmp_003364"
     CDS             16820..16963
                     /gene="ypaB"
                     /locus_tag="ACHKNB_RS16820"
                     /old_locus_tag="pgaptmp_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HGU4196819.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4049 a         4194 c         4578 g         4316 t
ORIGIN      
        1 accaactgct aattatcgcc agaaatgtcg ctcaatttta cggtaattgt ctggttgcgc
       61 ttgtctatag gtggagttta cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa
      121 tatttcatca ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg
      181 agtagcaatg gtttgccacg tatcgacaat aaaacattgg ccagactggc acagcagcac
      241 ccggttgtcg ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg
      301 tcagataaaa caggtattac ggttaaaggt acccaggatg cccgtgaact gggcaacgct
      361 tttagcgctg acatccctga tttcgatctt tattccagta ataccgtccg gaccattcag
      421 tcggcgacct ggttttccgc gggtaaaaag ctgacggtag ataaacgact tcttcagtgc
      481 ggtaatgaga tttatagtgc aattaaggac ttacaaagca aagcgcctga taaaaatatc
      541 gttattttca cccataatca ttgcctgaca tatattgcta aaaataagcg tgatgcgaca
      601 tttaaacctg attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg
      661 gatggggaat tcgttaacca ctaaattttc aatctgacag ccagtgatgg ctgtcatcat
      721 tgttaaagaa gacctttcaa acgtaacgtt tttcgggtgg caacatttaa atcataatgc
      781 accagatctt caggttttac ccacgcgtag tcctgaaact cttcgtttat tttcacttct
      841 cggttggcag aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca
      901 tctgcatacg tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa
      961 agcagctatt ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg
     1021 ccagactcca cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg
     1081 gccattttac acagcaaata agcaccatca ttttgaatca aagggcatac aatagtccgt
     1141 tgtcgcatag gggtattcct tataaataaa aagaaaccat gagaatacgg taactcagaa
     1201 atatgtgaaa gtgacagtaa tctcttttgc aatgataact gcacttatta ttatttttgt
     1261 gtgtatcggt aaagtgtgtt tattttttcg gcgtttaatt atttaagaag tatttagtta
     1321 caataagtga ctagatccct tttttcgctc attgaatcct ctgtgccagg cagtacaatc
     1381 ccgcagcttt gggaattttg attaattgct tgtgggaaag gggataatga aaaaaattgc
     1441 gctagcaggt ctggccggaa tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag
     1501 cggccaggcg ggtaaagaat acaccaatat tggtgtcggt tttgggactg aatcgacggg
     1561 cctggcttta agcggtaact ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg
     1621 gctggggttg aatctgcctc tcgggccgtt aatggcgacc gttggcggaa aaggcgttta
     1681 caccaacccg aattacggcg atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg
     1741 gaaaattggc aacagcttcc gtttgtttgg cgagtattac tactctccgg attcgctctc
     1801 cagcggtatt caaagttatg aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc
     1861 agtcagtatt gaggcgggtt atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga
     1921 caacgccgtg gctgacggcc cgtatgttgg ggttaacgcc agtttctgat cccttccggc
     1981 gcggcattct gtcgcgccat tcagtttctt actacgcctt atctcccttt gcgttatagt
     2041 gattcgataa caaaagcagg agataaccat gttaaaagtg gaaatgctat ccaccgggga
     2101 tgaagtgtta cacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt
     2161 tcatcagggg ctgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt
     2221 caccattctt cgcgaacgta gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg
     2281 accgaccagc gatgatttaa gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt
     2341 gctgcatgaa gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat
     2401 ggcaccgagc aaccgtaaac aagcggagct gcctgccagt gctgaattta tcaataaccc
     2461 ggtaggcacc gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc
     2521 cggcgtaccg tcagaattta aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga
     2581 gcgtttttct ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga
     2641 aagcgatctg gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta
     2701 tcgctcttca atgccgatta tcgaactgaa actcaccgga ccggcaagcg agcaacaggc
     2761 gatggaaaaa ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg
     2821 caccgaagga ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac
     2881 gttgagcgag caattcaccg gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc
     2941 attgctggcg tgtgaagtgg ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg
     3001 gattacagaa cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga
     3061 gaacgaacat ctcaactttg cgctagccac accagacggc actttcgctc tgcgtgtgcg
     3121 tttcagcact acgcgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact
     3181 gaatatgctg cgccgttggt taaacggcca ggatatcgcc agtgagcatg gctggattga
     3241 ggttattgag tccatgacct tatctgtctg attccgccac tctgtgccgg tttactgcac
     3301 cggcacattc cccctccggc aaaacttgtc caaccctccc tacggtgagc gtggtgttgg
     3361 ggccagtgtg gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagttaa
     3421 cgattcactt tgtctgttgg tgcaggtgga aagtaaaacg gcactggata acctggacga
     3481 gatcctcgac gtcgaaggga ttgatggcgt gtttattgga ccagcggatc tttctgcgtc
     3541 gttgggctac ccggataacg ccgggcaacc ggaagtgcag cgaattattg aaaccagcat
     3601 tcggcggatc cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc
     3661 gcagcaatgc ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta
     3721 cagcgatgcc ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat
     3781 aaaaggtagt tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag
     3841 tggttattcg ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga
     3901 tcaatgccct gttccagcat gcgcaaagca attttaatgg cttgttcatg cataccttcc
     3961 tgccagccaa tttgatgccc ttcctgccgt agccgttcag caatagtcat taatctctcc
     4021 ttttgtagtg gtgaacgttc ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg
     4081 ccgcattgca gcagataatt aaacagtgtt tgtagctggc tgtcattagt gaagcctcta
     4141 accaaaagcg tggtgatcct gtcaaccatg ccgattaaat cgcggtcgcg aatatgcttt
     4201 tgaatcagtt ccagcagagc tatacgccga tgttgcatga tctcatcgtc aggtacgatg
     4261 gtgatatcca ccaacggaaa agcttcggtg tacaactgcc ggccaagttg cggatcgtca
     4321 aactcatcca gccagttaag tgagtacggg tagggcgatg tttcgccatg ataaaacagc
     4381 aatggcacca ccagcggaac tctgtcatag cctttatcca gctgacgctg catggcggca
     4441 gtggcatagc gcattagccg aaaagccata ttcttttctg cagagctttg atgttcaatc
     4501 acgcagtaga tatagccgtc accgtcgctg gtttccacgg accacaaaac atccgagtag
     4561 taagcgcgta aacttttttc aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg
     4621 caaagcttgc gcagtggttc tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt
     4681 gtgaacataa aggttttaaa caccgcatca tgcggggagg atgttgttga ttcggtcatg
     4741 gttgtccgtc acctgatatt tatgatgacg cgacattacg tctatcccgg gcctttgaca
     4801 tctgtcagtt tcagcctttg cgagacattt tcagaggaat tttcaaagcg ttgcaacgca
     4861 tacatggtgc gtggaatcgc gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca
     4921 tcaaaaggtc ggttaagcca gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag
     4981 gctcgtggac atctcaatct gccatttgca ggtttcgcag tcggtgatga ccagatccgc
     5041 gccgctctct tctatctggc ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt
     5101 ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca gcaccgttaa
     5161 ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc ccattttttc
     5221 catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt
     5281 gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat cgcgcaagcc
     5341 tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg tacaggttga
     5401 ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg actctacatt
     5461 cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg gtacgccgca
     5521 gcatttttct ttgctgagca gttgtacacc ggtacccatt gcgttgagca ctttaattaa
     5581 atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg
     5641 gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgga acgtgccgaa
     5701 ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac gccgcatcaa gcagctgccg
     5761 caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg aaacgctacc
     5821 catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt
     5881 tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg cgacttcaca
     5941 acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg
     6001 cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat tcacccggct
     6061 caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt
     6121 cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc actgacggca
     6181 caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg
     6241 gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctc atcagtggtt
     6301 acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc
     6361 gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt acgttccgct
     6421 accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga
     6481 atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca ggtcactttt
     6541 ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg ctgtaactgg
     6601 ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa
     6661 ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc
     6721 atcaggatca tttcgcaggt attggcgaca gtaataagcg catcaagtaa cagcggccag
     6781 ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt ggcgttattg
     6841 cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag atccagttcg
     6901 cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat ccccactaca
     6961 catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa ccaggttgca
     7021 cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc
     7081 gcaccgcttt cagcgatcaa tgcctgcgct tggcaagcga gatcgagcac gcgttgtggt
     7141 tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag tccactgtga
     7201 atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga accggatgag
     7261 aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg tttttgcagt
     7321 tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc
     7381 tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa attcgctttc
     7441 gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt taaggaaggt
     7501 ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc
     7561 agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg tacgacggcg
     7621 taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgaactt cacccgcagt
     7681 taccgcttcg cactcacata ccaggctacg atgcagacgg ccttcgctca gccaggcagg
     7741 cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca gggagatgac
     7801 tttacgcaag gtaacttcag cgggttcttg tgaaccaggc agtgccagat cggcagtcgt
     7861 acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt cagccatcag
     7921 ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac catcgcgttc
     7981 agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt cgtcatcgct
     8041 ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg
     8101 ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca ctcgattatc
     8161 gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc
     8221 aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat gctggttaat
     8281 gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc gcagatcggc
     8341 atattcggca atgtgttgcc cccagatccc agcggcatta accacgacag gtgcatgaag
     8401 ggcctgagtt tcgccggtga gatgattacg tacacgaaca ccgcacaccg tcgcgccttc
     8461 acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt ctttggcatc
     8521 cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc
     8581 gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg ggtctatagc
     8641 ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc
     8701 atcttccggc agggtgataa acaggccgtt ggttggttca acgcagtgac gtgcaatgcg
     8761 tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg ttaccgcata
     8821 gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg
     8881 gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc
     8941 cgttgcgccg ccaccaatga taatcacgtc acttgattgc gagtcgcgag ttttcattgt
     9001 tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa ttgtttgatt
     9061 tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat
     9121 tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttctg aatgttttct
     9181 taacatcgcg gcaactaaag aaacggcagg ttctcccact gaatcagtct gttaatcata
     9241 aataagacca cgggccacgg aggctttcaa tgttgagtat ttttaaacca gcgccacaca
     9301 aagcgcgctt acctgctgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt
     9361 tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac tttgcgcttg
     9421 ctatgcctta tctggtcgag cagggattct ctcgcggtga tttaggtttc gcgctttcgg
     9481 ggatctcgat cgcttatgga ttttcgaaat tcataatggg ttcggtatcg gatcgctcga
     9541 atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg
     9601 gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg
     9661 gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc
     9721 agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta
     9781 ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat gcggcgctct
     9841 atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg atgcgcgata
     9901 ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat ccggacgact
     9961 ataacgaaaa agcggaacag gagctgacgg cgaagcaaat cttcatgcag tacgtactgc
    10021 cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg
    10081 gcatcctcga ctggtcaccg acttacctga aagaggttaa acatttcgcg ctggataaat
    10141 cttcctgggc ctacttcttc tatgagtatg cgggtattcc gggcaccctg ctgtgcggct
    10201 ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc tttatgacac
    10261 tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata
    10321 tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg atcggtctgc
    10381 atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt accgggctgt
    10441 ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct
    10501 tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt
    10561 tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg
    10621 gaggctaatg gcatgaaatt gaagctgaaa aaccttagca tggcgatcat gatgagcact
    10681 atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat
    10741 cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg
    10801 cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt
    10861 ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc ggatcgggcg
    10921 cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa
    10981 tttaccgaag gtttcgatat tgaaaacggt aaaaaagtac agacttatcc ggggcgtttc
    11041 ccaatgggta agtccgactt ccgggtgcac acctttgaag aagagattga atttgttcag
    11101 gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaattaa agcgccgtgg
    11161 ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat
    11221 ggttacaccg gtaaggacga caaagtttat ttgcaatgtt ttgatgctga tgagctgaag
    11281 cgtattaaga atgagctgga acccaaaatg ggcatggatc tcaatctggt acagctgatt
    11341 gcctataccg actggaatga aacgcagcag aaacagccgg atggaagctg ggttaattac
    11401 aactacgact ggatgtttaa gccgggtgct atgaaacagg tggcggaata tgccgacggt
    11461 atcgggccgg attaccatat gttgattgag gaaacatcgc agccgggtaa tatcaaactc
    11521 actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta taccgtgcgg
    11581 tcagataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc tctgtataac
    11641 aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa attccttaat
    11701 aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcaca tcaaattttt
    11761 ccattcacca cgagttgttc cgtacacgtt tgtagtaaaa acagtggact gccttctacc
    11821 ggcttttctt tagccaacaa cgaaatctgt cgatagaagg ttggttcgtg aggaacggaa
    11881 cacaattcac ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat gcctaatccc
    11941 tgtcgaataa acttcatcgc tgtatccgac tgattaaatt cacattttat ctactgggcg
    12001 cactattagt ggtagtggct ggtggaatct atgcgctgca aatgaagggt aaaagcggtg
    12061 tcacgctagt agtggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc
    12121 aggtaaccac ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg tatggcagtt
    12181 tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag
    12241 cctaactatc gccgtaatgg tatgagcggg gtgcaatgtg tcgagcgcaa cggcaaaaag
    12301 ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga
    12361 ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg
    12421 ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg
    12481 actgaagata tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg
    12541 ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa
    12601 atgcatagca ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca
    12661 gaaggtaatg cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc
    12721 gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa
    12781 gcggactggg agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa
    12841 gacgcataag cgttgtatct ggcactacga ccaattcaca tctcgatttc aatatcgcct
    12901 tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca
    12961 atccagtcaa cctgacctgc aaccagacgg gtgcgacagg agccgcagta accttcgcga
    13021 cactgatact caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca
    13081 tcctggcaca gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagttgga
    13141 agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga
    13201 cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccatg
    13261 ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat
    13321 tggtgatgta ttcaacgtac tggcagagaa tatctttatt cagaccaatc atcgaaccgt
    13381 cacggaacag ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat
    13441 agcactcctg cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc
    13501 gcagcagatt cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca
    13561 ggcgaataat tttggcgttg ccttccatca attcgcgttc tgcaaatgcg aaggaacaag
    13621 caaagctgac gtagaaacga atcgcttcca gcgcgttaac gctcatcagg cagagataca
    13681 gttttttctt cagctcgcgc aggctaacgg tcacagtttt accgttaacg gtgtgggtac
    13741 cttcgcccag cagatgccag tagctggtca tttcgatcag ctcatcgtaa tagctggaga
    13801 tcccttccgc acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaacagacg
    13861 gatcgttaac gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga
    13921 acgcccaggt ttcgacccag gtctccagtt ccgggatcga gatcagcggc agcagtgcca
    13981 cgttcgggct acggccctga atggaatcca gcagcgtctg atatttcagg ttgctgataa
    14041 agatgtgttt ttcgtgttca ggcagtgcct ggtaatcgat acggtcgcga gaaacgtcaa
    14101 cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat
    14161 atttttgctg atcgtagcga gccacgttga ccggctgacc aaagaacatc ggttctttga
    14221 gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgttgggagt
    14281 ttaatgccgg ataaggcatt ttacgccgca tcctgtgcct gatgcgacgc tgacgcgtct
    14341 tatcaggcct acaaatccga gccgtaggcc ggataaggcg ttcacgctgc atccggcatc
    14401 tcaatatcag atcttacatg cgccgctttc gcagccatcg tcctggattg acggtaccag
    14461 atcgtcctgc gcgtcttcag cgccgtcacg ggtgttctga taatacagcg ttttgacccc
    14521 gaatttgtag gcggtgagca ggtctttcag caactgctgc atcggcactt ttcctgacgg
    14581 gaaacgtgac ggatcgtagt tggtgttagc agagatcgac tgatcgataa atttctgcat
    14641 gatacctacc agttgcaggt agccatcgtt acccggcatt tcccacagca gctcataggc
    14701 gtcgtgcagg tgctcgtagt ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt
    14761 gatgctgacg taaccgcgcg gcggttcaat accgttagtg gcgttagaga tctgtgaaga
    14821 agtctctgac ggcatcaggg cagaaagcgt ggagttacgc aggccgtgcg ttttgattga
    14881 ttcacgcaat gcttcccagt cgtaatgaag cggctcatta gcgatggtat ccagatcttt
    14941 cttataggta tcgatcggca ggatcccttt cgcgtaagtg gtttcgttaa accacgggca
    15001 cgcgccttgc tcttttgcca gctcgttaga cgctttcagc aggtaatact gaatcgcttc
    15061 gaaggtttta tgcgtcaggt tgttggcgct gccgtcggag taacgcacac cgtgcttcgc
    15121 caggtagtaa gcgaagttga tcacaccaat acccagcgta cgacgaccca tcgctccacg
    15181 tttggcggcc gggatcgggt aatcctgata atccagcagc gcgtcaagtg cacgaaccgc
    15241 cagaattgcc agctcttcca gttcatccag gctattaatt gcgcccaggt tgaaagcaga
    15301 cagcgtacac agcgcgattt caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg
    15361 cagggctatc tccaggcaca ggttagactg acgcactggc gcgatggccg gatcaaacgg
    15421 gctatgggta ttgcagtggt caacgttctg aatatagata cgaccggtag acgcacgttc
    15481 ctgcatcatc agcgagaaca gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc
    15541 tttctcatat ttggtataca ggcgttcaaa ctcttcctga tcggcgaaga atgcgtcgta
    15601 cagccccggt acgtcggacg ggctgaacag ggtgatatct tcacctttca gcaggcgggt
    15661 atacatcagt ttgttgatct gtaccccgta gtccatatgg cgcacgcggt tgccttccac
    15721 accacggttg tttttcaaca ccagcaggct ttccacttcc agatgccaca tcgggtagaa
    15781 cagcgttgcc gcaccgccgc gcacgccgcc ctgagagcag gatttcaccg ctgtctggaa
    15841 atgtttgtag aacggaatac agccggtatg gaacgcttca ccaccgcgaa tcgggctacc
    15901 cagcgcacga atacgcccgg cgttgatgcc gatcccggca cgctgggaaa cgtatttcac
    15961 aatcgcgctg gaggtagcgt tgatggaatc cagactgtca ccgcactcga tcagtacgca
    16021 ggagctgaac tgacgagtcg gggtacgcac gccggacatg attggcgtcg gcagcgaaat
    16081 tttaaatgtg gaaaccgcgt cgtaaaaacg cttcacatat tgcaggcgcg tttcacgcgg
    16141 gtagttcgag aacaggcacg cggcaaccag aatataaagg aactgggcgc tttcgtagat
    16201 ttcgccggtc acgcggttct gtaccagata tttgccttcc agctgcttaa cggcagcata
    16261 agagaaggtc atatcacggt cgtgatcgat aaacgtgtcc atctgcttga actcttcttc
    16321 cgtgtagtct tccagcagat gattatcgta tttgcccatc tcgaccattt tcaccacgtg
    16381 gtcgtacagc gcaggcggct caaactggcc gtaggctttt ttacgcaggt ggaagatcgc
    16441 caggcgcgcg gcgagatact gataatccgg cgcatcacgg gagatcaggt ctgcggccgc
    16501 cttgataatg gtttcgtgga tgtcagaggt cttgataccg tcataaaact gaatgtggga
    16561 gcgcagctcg acctgggaaa tcgaaacgtt atgcagtcct tctgccgccc aatccagaac
    16621 gcgatggatt ttgtcgagat tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag
    16681 attctgattc atgtatgtcg tacctgtttt tggaattctt tccgcaatag tttcatgcag
    16741 actacctgta gtaagggaag ctgcttagaa cactatatat agggggtatg tttgggattc
    16801 actgcaagat agtgtgaaaa tggccctctt gcaagtgcat aactttgtgg ataactcagg
    16861 aaggaaaaag tggctttcgc gcaccttagg tcagacaagg tgtccgggaa agtcaatggg
    16921 aagagaaaaa tttgttaaaa ataactgttc ggcgatttct tgaacggcag aataggttgg
    16981 ataaattgat atagatggtt tattaatctt tttgctaatg attgcaaaaa ttgatctaag
    17041 cacgagtcat cacataatct gatgtatcag ttgtggtcaa tatataaacg aatgtaagcg
    17101 attgttgatg tcgaaatggt tgttttctat tcggtta
//
LOCUS       DAZFVO020000109        17130 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFVO020000109 DAZFVO020000000
VERSION     DAZFVO020000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.110"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            68..343
                     /gene="ypdI"
                     /locus_tag="ACHKNB_RS16825"
                     /old_locus_tag="pgaptmp_003365"
     CDS             68..343
                     /gene="ypdI"
                     /locus_tag="ACHKNB_RS16825"
                     /old_locus_tag="pgaptmp_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HGU4196820.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(420..662)
                     /gene="yfdY"
                     /locus_tag="ACHKNB_RS16830"
                     /old_locus_tag="pgaptmp_003366"
     CDS             complement(420..662)
                     /gene="yfdY"
                     /locus_tag="ACHKNB_RS16830"
                     /old_locus_tag="pgaptmp_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HGU4196821.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1015..1935
                     /gene="lpxP"
                     /locus_tag="ACHKNB_RS16835"
                     /old_locus_tag="pgaptmp_003367"
     CDS             1015..1935
                     /gene="lpxP"
                     /locus_tag="ACHKNB_RS16835"
                     /old_locus_tag="pgaptmp_003367"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HGU4196822.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFINPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2291..2362
                     /gene="ypdK"
                     /locus_tag="ACHKNB_RS16840"
                     /old_locus_tag="pgaptmp_003368"
     CDS             2291..2362
                     /gene="ypdK"
                     /locus_tag="ACHKNB_RS16840"
                     /old_locus_tag="pgaptmp_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HGU4196823.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2427..3665)
                     /gene="alaC"
                     /locus_tag="ACHKNB_RS16845"
                     /old_locus_tag="pgaptmp_003369"
     CDS             complement(2427..3665)
                     /gene="alaC"
                     /locus_tag="ACHKNB_RS16845"
                     /old_locus_tag="pgaptmp_003369"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HGU4196824.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4042..5739
                     /gene="ypdA"
                     /locus_tag="ACHKNB_RS16850"
                     /old_locus_tag="pgaptmp_003370"
     CDS             4042..5739
                     /gene="ypdA"
                     /locus_tag="ACHKNB_RS16850"
                     /old_locus_tag="pgaptmp_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HGU4196825.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5754..6488
                     /gene="ypdB"
                     /locus_tag="ACHKNB_RS16855"
                     /old_locus_tag="pgaptmp_003371"
     CDS             5754..6488
                     /gene="ypdB"
                     /locus_tag="ACHKNB_RS16855"
                     /old_locus_tag="pgaptmp_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HGU4196826.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6501..7358
                     /gene="ypdC"
                     /locus_tag="ACHKNB_RS16860"
                     /old_locus_tag="pgaptmp_003372"
     CDS             6501..7358
                     /gene="ypdC"
                     /locus_tag="ACHKNB_RS16860"
                     /old_locus_tag="pgaptmp_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4196827.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7361..9856)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS16865"
                     /old_locus_tag="pgaptmp_003373"
     CDS             complement(7361..9856)
                     /gene="ptsP"
                     /locus_tag="ACHKNB_RS16865"
                     /old_locus_tag="pgaptmp_003373"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGU4196828.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAALFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9881..10918)
                     /gene="ypdE"
                     /locus_tag="ACHKNB_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /pseudo
     CDS             complement(9881..10918)
                     /gene="ypdE"
                     /locus_tag="ACHKNB_RS16870"
                     /old_locus_tag="pgaptmp_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(10918..12003)
                     /gene="ypdF"
                     /locus_tag="ACHKNB_RS16875"
                     /old_locus_tag="pgaptmp_003375"
     CDS             complement(10918..12003)
                     /gene="ypdF"
                     /locus_tag="ACHKNB_RS16875"
                     /old_locus_tag="pgaptmp_003375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4196829.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12018..13265)
                     /gene="fryC"
                     /locus_tag="ACHKNB_RS16880"
                     /old_locus_tag="pgaptmp_003376"
     CDS             complement(12018..13265)
                     /gene="fryC"
                     /locus_tag="ACHKNB_RS16880"
                     /old_locus_tag="pgaptmp_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGU4196830.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13287..13613)
                     /gene="fryB"
                     /locus_tag="ACHKNB_RS16885"
                     /old_locus_tag="pgaptmp_003377"
     CDS             complement(13287..13613)
                     /gene="fryB"
                     /locus_tag="ACHKNB_RS16885"
                     /old_locus_tag="pgaptmp_003377"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGU4196831.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13832..14797)
                     /gene="glk"
                     /locus_tag="ACHKNB_RS16890"
                     /old_locus_tag="pgaptmp_003378"
     CDS             complement(13832..14797)
                     /gene="glk"
                     /locus_tag="ACHKNB_RS16890"
                     /old_locus_tag="pgaptmp_003378"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HGU4196832.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15001..16257
                     /gene="yfeO"
                     /locus_tag="ACHKNB_RS16895"
                     /old_locus_tag="pgaptmp_003379"
     CDS             15001..16257
                     /gene="yfeO"
                     /locus_tag="ACHKNB_RS16895"
                     /old_locus_tag="pgaptmp_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HGU4196833.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16372..16698
                     /gene="ypeC"
                     /locus_tag="ACHKNB_RS16900"
                     /old_locus_tag="pgaptmp_003380"
     CDS             16372..16698
                     /gene="ypeC"
                     /locus_tag="ACHKNB_RS16900"
                     /old_locus_tag="pgaptmp_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HGU4196834.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16839..>17130)
                     /locus_tag="ACHKNB_RS16905"
                     /old_locus_tag="pgaptmp_003381"
     CDS             complement(16839..>17130)
                     /locus_tag="ACHKNB_RS16905"
                     /old_locus_tag="pgaptmp_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HGU4196835.1"
                     /translation="FIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGI
                     ALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT         4151 a         4575 c         4291 g         4113 t
ORIGIN      
        1 ttaattatat gtaaatacag ccataacatc gtaggttctc agtgaaccat tttcaggata
       61 actttctatg aaagtaaact taatactttt cagcttattt ttattggtct ctattatggc
      121 atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct ataaagagac
      181 tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat cacaggcgat
      241 tttgtttaag atgagagaag atgcgccacc attaaacctc acagaagaaa tcacgcctac
      301 ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt cacatggcag
      361 gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat cctgacccac
      421 tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg ggcaagaaac
      481 ataaatatcc ccgtcactag ttcattaccg ccagttaacc acatggtgaa gtaataaatg
      541 agtgcagcga ggatcaccat gccgaagaaa gaagagactg ccgataccac gttcagtacc
      601 tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca taaataaatc
      661 atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta atagtaagac
      721 aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcatgt taaatgcaag
      781 actgttgtgt acggaaaaat atttactttg cacgattaat aatcagtagc tgaaagcagt
      841 cagcgagtga taaagtacag ataccgcatt catgctcctc gtggttatgt cctgactagt
      901 ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt gagtgtaccg
      961 ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt aactatgttt
     1021 ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac atggtttggg
     1081 cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt tctcggcacg
     1141 cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc ccgtaaaaac
     1201 ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat tgccgaaaac
     1261 tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg gcccgacagt
     1321 cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc acaaatgcaa
     1381 aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg cggccgcgtg
     1441 atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca gctgatggaa
     1501 tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag aaataatctg
     1561 cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc cgatcaggat
     1621 tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt cgccacaacc
     1681 aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt aacgatggta
     1741 agaaaagcgg attacagcgg atatcgtttg ttcatcaacc cagagatgga aggctacccg
     1801 acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga gatcatgcgc
     1861 gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt gggagaatcg
     1921 tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaattggc cataataacc
     1981 tcatggtttt tatggctttt ttcttaaggt tttttccttc gctgaatatc taattattac
     2041 tgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa gtaaataaga
     2101 aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg agcagtttag
     2161 ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc gctttgcccg
     2221 gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta attacgcgga
     2281 gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg tttgggccgg
     2341 tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag ccatcaggct
     2401 cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg tttgctgctg
     2461 gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc ctgacgaata
     2521 cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc gccaaagcca
     2581 atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa ttccagcgat
     2641 cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc cttcggcatt
     2701 tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacggcg gcgtttgtac
     2761 tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat cgccgccacc
     2821 tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag ggcactgacc
     2881 agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta gcttttcgac
     2941 agcgtaaaga actcgactgc cacatcgcgt gcacccggta cctgcataat tgacggcgct
     3001 ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag cacatcgtag
     3061 cgtttcgcca gcgccactac tttttcgaag aactccagtt ccacgcattg cgcggttggg
     3121 tttgacggga agccgaggat catcatcttc ggtttcggat aactttcacg aatggcgcgc
     3181 tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac ctgcgccccg
     3241 gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag caccgtgtca
     3301 ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc aatagtgacg
     3361 atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca gcgggaaatg
     3421 gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc cgggcgctgg
     3481 gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg gttacccatg
     3541 ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc agcggtgatg
     3601 ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg gcgagtgtca
     3661 gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc tgccacaatg
     3721 gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta gaacattttt
     3781 atcaatggct ttaaatgagg aactccactt caaaaacagg aaatttttct acgctaacaa
     3841 gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag ccagaagtgc
     3901 attatcaaga gatacattgc taatacacca tttatctttc aatccccttt taaatttatg
     3961 gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc atagaggttt
     4021 aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc ggtcttcgat
     4081 cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct gtttcgcgaa
     4141 ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc cattttctcg
     4201 ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct ggtgaacgta
     4261 cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg catcattacc
     4321 ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac ggcgatcccc
     4381 tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct gaaaatcccc
     4441 aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac gttgaccatg
     4501 attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt ctctaaaatc
     4561 ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct ggtgcaaagc
     4621 gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct ggatatcgcc
     4681 aacaaaacgc tacctctgtt tcgccatgtc aatagcgagt cattacgcaa agtttgtgaa
     4741 attattcgcg atgacattca cgccgatgcg gtggcgatta ccaataccga tcatgtgctg
     4801 gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat tagcccgact
     4861 acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga agcccaccgc
     4921 acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggcgt tgtgaccgga
     4981 acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca ggaaatggcg
     5041 gtcggtctgt cgcaaatcat ctccacccaa ctggaggttt cacgcgccga gcagctacgt
     5101 gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc ccatttcctg
     5161 tttaacgctt tgaacgctat ttcatcatca atccgtctga atccggatac tgctcgccag
     5221 ctgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga cgatgagcaa
     5281 atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga gcaggcccgc
     5341 tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg ctgcattccc
     5401 agcctgctga tccagccgtt ggtggagaat gccattgtcc acggtattca gccttgcaaa
     5461 ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggca atcgggtacg cattgcggtg
     5521 cgggataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc gaatgaaatg
     5581 ccgggcaata aaattggcct gctgaatgtt catcatcgcg tgaagttatt gtatggcgag
     5641 gggctgcata tccgccgcct ggaaccagga acggaaattg cgttttacat tcctaaccaa
     5701 cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat attgtgaaag
     5761 tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta attaaagagc
     5821 acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg aagtttttgc
     5881 agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg gatggcgtgt
     5941 tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt
     6001 ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt
     6061 atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg caacaacagc
     6121 agaccagcag tacgcctgcc gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga
     6181 aagatgagcg aataatcgtc acgccaatta acgatatcta ttacgccgaa gcgcacgaga
     6241 aaatgacctt tgtctatacg cggcgtgaat cctacgtaat gccgatgaac attaccgaat
     6301 tttgcagcaa actgccgccg tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc
     6361 tgaacaaaat acgcgaaatc gaaccgtggt ttaataacac ctacattctg cgactgaaag
     6421 atctggattt tgaagtgccg gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc
     6481 atctttaatc gggaactttc atgaaagcac caggcttgcc cgccgatcaa caatttttcg
     6541 ccgatctgtt cagcggcctg gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca
     6601 gccagcctgc ctcgttaccg gtgggcagtt tatgtattga ttttccccgt ctggatatcg
     6661 tgctgcgcgg cgaatacggc aacctgctgg aagcaaagca gcagcgaatg gtggaaggag
     6721 aaatgctgtt tattccggca cgcgcggcta atttaccgat caacaacaaa ccggtgatgc
     6781 tgttaagcct ggtgttcgca ccgacctggc ttgggttatc gttttacgat agccgcacca
     6841 cgtcgttgtt gcatcctgct cgccagatcc agcttcccag cctgcaacgc ggtgaaggtg
     6901 aagcgatgct taccgccctc acccatctta gccgttcgcc gctggagcag aatatcattc
     6961 agccactggt gttaagtttg ctgcatcttt gccgtaacgt ggtgaatatg ccgccgggca
     7021 attcgcagcc gcgcggcgat tttctctatc acagcatttg taactgggtt caggataatt
     7081 atgcccagcc gctcacccgc gagagcgtgg cgcagttttt taatatcacg cccaatcatc
     7141 tgtcaaaact gtttgctcag catggaacga tgcgttttat cgagtatgtg cgttgggtgc
     7201 gaatggcgaa ggcgaggatg attttgcaga aatatcatct gtcaattcat gaagtggcac
     7261 agcgttgcgg ttttccggat agcgactatt tttgtcgcgt tttccggcgt cagtttggtc
     7321 tgacgccggg agagtacagc gcccgttttc agggctaacg tcagaaggtt aattctgttt
     7381 ccagcagcgt caggatactt tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt
     7441 tattcaccag tttacgcgcc agctgcgaga agactttcac atgattcatc ccttcgttag
     7501 cacccagcgt cagcatgatc accagttcga cttcgcccat ttctgactgc cagtcaatcg
     7561 gtttcgccag ccgggcaatg ctgatactgg aatgacggat ccactgagat ttggtgtgcg
     7621 ggatcgctac gccaaaacca acgccggtgg taacaatctc ttcccgctgc cagacatctt
     7681 cttccagctc aaacggatgt tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact
     7741 ggatcgcctg ctctttattg ctaaaatcct gatcaacaaa gatattctcc agcgccagca
     7801 gtgggcgaac gtcttcttcc ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac
     7861 tgcggcattc acatgcctga cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa
     7921 gctggctttt caccgccgga atacgcgggc tactcatact cagctcatcc aggcccagcc
     7981 caagcagtag cggcagataa cggctttcac cgcccagttc accgcaaatg cctacccatt
     8041 tgccccgctg atgcgcagtg gtaacaatct gctgcaacat gcgcaggaac gatggcgtaa
     8101 tcgggttata cagcggcgat acgcgcgggt tattacgatc gaccgcatac agatactggg
     8161 tcatatcgtt agaaccgata ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt
     8221 aacacaccga cggcacttcc accatgatac ccagagtaat cgtctctgca tgacgcaggc
     8281 catcgcgctt aagctcaacg atcgcttttt ggatctcgcc cttcacccat aagatctgat
     8341 cgaggctgtg aaccatcggg atcatcaact gggcgttgcc aaaactggca gcgcgcaaaa
     8401 tcgcccgtag ttgagtgcgg aacagggcag caaattccgg gtaaatacgt accgcgcgat
     8461 agccgaggaa cgggttctct tcctggggaa tattcagata aggaatgctt ttatcgcctc
     8521 cgatgtccat tgtgcggaag ataatcggct tgtcgcccgc tgccagtagc acctgctggt
     8581 aggcttcaaa ttgctcctgc tcgtccggcg cgctgtcacg atccatatac agcatttcgg
     8641 tacggaacag cccgacgcct tccgcgccgt tggcaaacgc gcctggtgct tccagagcgg
     8701 tgccaatatt cgccgcgata tcaatacgct tgttatcacg ggaataggcc agctgcgcgg
     8761 cagcctgcgc ctgttgtttt tggcgtttat ccgccagcgt ctgcgcaacc tgataataac
     8821 cgctcaccgc gtcattcggg ttaatcgcca gcacgccgca ctgggcgtca agcaccgccg
     8881 gttgcccggc ataacgggca atcgcatcca gcggcaggcc gctcagtacc gggatcgccg
     8941 aggcgcgggc cagaatcagg gtatgcgagg tgcgcccggt tttctccaga atcatgcccg
     9001 caagattttt caaatcaagg ctcaaaaact ggcttggggt taaatcttca gccaccagaa
     9061 tggtcggttt ttccagcacc agattgttgc gcggcttcag ttccggccag gtgatatgca
     9121 gcaactgttc gctaatatcg cgaatgtcgc taacacgttc gcgcagataa tcgctggcag
     9181 aagcagaaag cttggcgcaa acctgctcca tattgctgat gatcgccgcc cccagtccct
     9241 gatgctgttc tgtcatcagg cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg
     9301 acaaatgagc gctgaggata gttttgcttt cgccgtcacg ctcacgcaat tgctggttca
     9361 gttgctcggc aagcgttgcc aggctgtgct ccagccgggt ggaatcttgc gcactggcgg
     9421 ggattgcccg ataactgtcg aggctgtcgc tttgtaacag ggtcagcgta cccacgccga
     9481 cgccgcttgc cagcacattg ccgtacagta aatccgggtt aaggcggctt aatgaacgcg
     9541 gcagcggatg cgccgtcagt tctgcctgcg taggctgaac gctgtcgctg tcgataaagc
     9601 gcacctggat gtactcttcc agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt
     9661 tcaggctgca actgtcatta aatagggtgc cggtgccaat cagcgccagc gaacttttcg
     9721 catcggcctt tgcgttctgg cgatggttaa taaaggtgac ttcactttgc cactggctgc
     9781 actgttcttt aagttcccac gccggacgag catgcagacc gttaggcaaa ggacagagaa
     9841 attgaatcgt taacataacc gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt
     9901 gaacaaccgt ctcacgggta agacgttgaa taagggcaga taaaagttgc tgcatctgca
     9961 aaatgtcgcg gcaatcggca atcgatgccg cacaatgtcc gtggcgcgtc gctggcccca
    10021 tcaccaccgt gggcacgccg gtgccggtta aatgcaccgc tccgccgtcc gtgccgccgt
    10081 tgctgaacat atccgcctgc aacggcacgc caatttctgc cgcgacggtt tcgatccagg
    10141 cggtaagttt tggcggcgca atcagcgact tgtcgcttaa caccagcatc ggcccgttac
    10201 caatctggcg atggttagcc gcgctataat caaagttttt cgcccagcag gcggtatcaa
    10261 gcacaatggc gacatccggc gacaccgcgc gggtggcagt ttgcccgccg cgtaatccca
    10321 cctcttcgct ggaacttgcc accagccaca cctccgcagg cagttcggcg tcgtgcagct
    10381 cacgtagtaa cgtcaccagc agatagcaac cgaggcggtc atcaaaggct ttccccatca
    10441 cccgctggtg agggagaacc tgaaaagttg tatcaaaggt gacgcgatcg cctggacgaa
    10501 ttcccgcctg catcacttcg tcatacgagc gcgcaccaat gtccacgcgc atggcgctga
    10561 cgtcattccc ctgccggtcg ccgtcaagca ggcctggaat tttgcactct tcacgggtgg
    10621 tgatgcgcac cggctgcagc tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt
    10681 caatagcgcc ttagcgggag atgctgcgca ccataaatcc cacttcgtcc atatgggcgc
    10741 agatcatcac cttcggacct gtcgattcat tgaggcggat cagcaccgat cccagaccat
    10801 caaatcgcac ttctttttgc aggcgatccg cttcttccag taggatctgc cgcacttcct
    10861 gttccgagga ggcgatcgca tctgcctcgc tcaacgcttt taatagcgat aaatccatta
    10921 tgcctctccc gtgagcaaca ctgttttcgg catggcgtag agcacttctg cgccttgcgg
    10981 ggtaaccagc acaacatctt cgatgcgcac gcccccttgc cctggcaaat aaatccccgg
    11041 ctccacggtc agcaacatgc ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg
    11101 atcttcatgg acttcaatgc cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa
    11161 tccagcctcg gtaatgaccc ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg
    11221 gcgaattgca gagattgccg cgagctgtgc ctgcaggaca atctgataga cgtcaaacag
    11281 cgggtgagat tcggcgctca ccccttcgcc attcaccagc aaggtgcgcg tcatatcaga
    11341 gcagtagccc tgatacagcg caccaaaatc gagagtgaca aactcgcccg ctgcaacaat
    11401 cttgtcgctg gctttgccgt gcggcagcgc cccgcgccag ccgctggcga caatggtgtc
    11461 aaaagaggct ttttctgcgc cctgctggcg cataaaccac tccagttcag cggctatctc
    11521 gcgctcgctc atccccgcct gaataaagcg gcgaatatgc tccgctccgc gatcggcaat
    11581 cccacaggcg aggcggatta tctccacctc ctctggcgtt ttgatttgcc gcagcacatc
    11641 cggcgtggcg ctaaccagtt tcgcattgag ttcagacttc cagcggtgcg cggtttccca
    11701 actcacctgc tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt
    11761 gacgatagtg gtaagcgtgt tcgtcgcgtc aagcaaatgc agctggtagc cttgcgcacg
    11821 ggcttccacc tcaacgaaat agcgcgaatc taccagaatg tgcgcacttt cacggctaat
    11881 aaccacatag cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga
    11941 gagaagcact gcatccagtt gttgcgcctt aagccagtcg cgcagcgaag cgagtaatgt
    12001 catctggatt ttccttctta caggctatcg attaacaatt tgcctttacg gaacatcatc
    12061 agacgcagga acaccaccat cagcgcagtt atgactgccc ccagcgcgat ccccgccata
    12121 tagacgccca ggttagtgac cagcggccat gcccagatag cggattccgg gaaccattgc
    12181 actgcgccca gccagacagc ggcggttgag ccgacaatcg cgccgaccat atacgacgga
    12241 atggcggtga tggggctttc cagcgcaaac ggaatcgcac cctcactaat ccccataaag
    12301 gcaaggaaca tcgcggtctt accctgcgga taaagttgag cgttgaacag gcgtttgccg
    12361 gttaaacgac ggtcaataat ggtcgccaga cccagaccaa tcggaggaat aacgatagcg
    12421 atagaacgag cggtgaccgg taatacgtgg tcggtggtaa agctgaaggc aacgaaacct
    12481 gccgctttgt tgattgggcc accaaggtcg attgcggtcg ccgcggcaat ccccattgca
    12541 tacatcagcg cgcccttctc acctgcggca gtcagcacgg tacggatacc gccgttgatc
    12601 cagccaccaa atggggtgat gacatagtac atcgccagca tgacaaaaat ggcggaaaga
    12661 atcggtagca aaaatgtggt tttgaacgct aacaggaaat ctggtaactg gattttctgg
    12721 ttcatccatt tcacaaggta accagcaacg atagatataa tcagcgcacc aatgaaggtg
    12781 gacggcaccg gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt cagcagttgc
    12841 gtcggctggg tagacatcaa gccgccgata aaaccagccg ggaacgccag tttgccgccg
    12901 atagagttcg ccacaaaagc ggcaaacatc ggaatagcaa agccaaacaa cacgccgccg
    12961 aaggactgcg acagccaggc aaatttcagt aaggagaggt cgaaaccgga gaatttcccg
    13021 ctattaagag catccatgat gccgatctca gcaggaattt taagccagct ataagcaatc
    13081 agctggctga aagcgaggat cacaccgccc ataattaagg tcggcaccat gcgtgaaatc
    13141 cctgacatca catgctgcgg gagttcaccc cagaagctgc ttttagaggc ctgcggattc
    13201 ttaactgccg ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg
    13261 gccataacaa ttatccctgt cgaatattac tgttgttcag aggcaatcat ctcttcgatt
    13321 tctttgatga tgcctacagc gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg
    13381 cgtgattcga aacgttcgtt atcttccggg gtaactgcca cggaatggat gatgatggtc
    13441 gcttcggcga tatcctgcgc cgtcaggcga ttctggatgc cgtccgcgcc ctgggtttca
    13501 attttcactt cataaccggc ttctaccgcc gcttcttcca gcgcctgagc ggccataaaa
    13561 gtgtgagcca ggcccatcgg gcaggcacat aaggcaatca gtttcttact catcgttaat
    13621 tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac
    13681 cagacaaata atgcatttac tggagaatta atcccgctgt atggaagtgt gacgcaggtc
    13741 ggccttgtga ggggacacaa agaaaaattg aattaactgt atgatttaaa agattatcgg
    13801 gagagttacc tcccgatata acaggaagga tttacagaat gtgacctaag gtctggcgta
    13861 aatgtgcacc ggaaccgaga agacccggat tgtcatggac gatgagatac accggaatat
    13921 catggacata ttctttaaag cgccctttat cttcaaatgc ggcacggaaa ccagaggctt
    13981 tgaagaactc aaggaagcgc ggcacgatac cgcccgcaat aaacacgccg ccaaatgtcc
    14041 cgagattgag cgccagattg ccgccaaaac ggcccataat gacgcaaaac agcgacaacg
    14101 cgcggcggca atcggtgcag ctatcagcca gcgcacgttc ggtaatatct tttggcttga
    14161 gattttctgg caggcggttg tcagctttca caattgcgcg atacaaattc accagcccag
    14221 ggccagaaag cacgcgttcc gccgaaacat ggccaatttc cgcacgcagt atttcgagga
    14281 taatggcctc ttcttcacta ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc
    14341 ttacccaacg cttatcgaca tggaccagat gcgcaacccc aagccccgtt ccggcaccgt
    14401 aaaccgcaat aggcttacct tctaccggtt ctgcgccacc aaactgaatc agatgctctt
    14461 ttttcagcat cgggatcgcc atcgatacag cggtaaaatc gttaataatt tccagatggc
    14521 taaaaccgag attctttttc atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg
    14581 ccacccagtc accggtaatt gggcaagcga tggcaataca gccgtctttc acctcgacct
    14641 tatgttcttc aagataaacg cgaatgaccg cttcgaggct ggggtaatca agccctgaat
    14701 aggtcttagc ctgcgagatt tcaccactgg caatatcaca cagagcaaga cgtgcgttgg
    14761 tgccgcccac atcaccgact aatgcatact ttgtcattct tcaactgctc cgctaaagtc
    14821 aaaataattc tttctcacac tgtaaatacc tgggggcata acaacaacgc cggaaaggca
    14881 ggctccctgt aaatatcgat ctgggtcaca catttacttt atcgtttcag caccaattgc
    14941 agcgatgcct ttttgcaagc tgggcaaact aagtatctga ccccgcataa ggaatagaac
    15001 atgctccatc cgcgagccag aaccatgttg ttattatcgc tccccgccgt ggcaattggg
    15061 attgcgtcca gtcttattct gattgtggtg atgaaaatcg cctcggcatt acagaatttg
    15121 ctctggcaac gactgccggg aactctgggg atagcccagg attcacctct ctggatcatc
    15181 ggtgtattaa cgctaacggg tattgcggtg gggttggtta tccgtttcag ccagggtcat
    15241 gccggaccag accccgcctg tgaaccgctg atcggcgcac cagttccgcc ctctgcgcta
    15301 ccaggactta tcgtagcatt aattctcggt cttgctggcg gcgtcagcct ggggccggaa
    15361 catccgatca tgaccgtcaa tatcgccctt gcggttgcca ttggcgctcg tctgttaccg
    15421 cgcgtcaacc gaatggagtg gactatttta gcctctgccg gaaccatcgg cgcgctgttt
    15481 ggtacacctg ttgcggcggc gttgatattt tcgcaaacct taaatggcag tagtgaagtt
    15541 ccgctatggg atcgtctttt tgcgccatta atggcggcag cagctggtgc acttactacc
    15601 ggattatttt tccatcccca tttttcactg cccattgcgc attacggaca gatggaaatg
    15661 accgatattc tcagcggtgc aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc
    15721 gtatggtgct taccacggtt gcacgcgatg atgaatcaaa tgaaaaatcc ggtgctcgtg
    15781 ctgggtattg gcggatttat tctcggtatt ctgggggtta ttggtggacc agtttcgctg
    15841 tttaaagggc tggatgagat gcagcagatg gtggcaaatc aggctttcag caccagcgat
    15901 tactttttgc tggcggtaat taaacttgcc gccctggtcg ttgctgccgc cagtggcttt
    15961 cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat
    16021 gagcacgtac ccgccgtacc agcggcaata accgtttctt gcgctattct cggcatcgtg
    16081 ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg cggcggtcgt tgtacccaat
    16141 accacattgc taccgctgct ctgtatcgtc atgcttccgg catggctgtt attagcaggt
    16201 aagccgatga tgatggtcaa tcgtccgaag caacagccac cccacgataa cgtttagcaa
    16261 aaaatgctct cctttatgat ttaagagtta tggcgatgat acggggcttt acctccccgt
    16321 aatattgcgt taacaggccg ctgacggctt cgatcgtgaa ggagaataac aatgttcagg
    16381 tcactgtttc tggcggccgc cctgatggca tttaccccgc ttgcagcaaa cgcaggtgaa
    16441 atcaccctac tgccatcaat caaattacaa attggcgatc gcgatcatta cggtaattac
    16501 tgggacggtg gtcactggcg cgaccgtgac tactggcatc gcaattatga gtggcgtaaa
    16561 aaccgctggt ggcgtcatga taatggctat catcgtggct gggataagcg taaagcgtat
    16621 gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc atcgcggaaa aggccgcgga
    16681 catggacacc gccattaaac acattcgcca atggaacaca atgcctgatg cgacgctagc
    16741 gagtcttatc aggcctacaa accggctctt taacgcgtag ggcggataag gcgtttacgc
    16801 cgcatccgcc aatagtgcca gatgcgacgc tcattcaact acaatcccag cgccgtcccc
    16861 accaacaacc agatattcag cgccacgacc agcactacaa tcacccagcc tgtctgtttt
    16921 acgcgtttgc tgttcaccag atcgcccatc aacttgctgt cactggtgaa aatcagcagt
    16981 ggaaccagcg ccagggcgat accaaaactt aacagcacct gactcataac cagaatccgt
    17041 gtcggatcta atcccatcag aatgacaata aatgacggca acatggtgac tgtacgacgc
    17101 acccacagcg ggatatggaa gcgaatgaat
//
LOCUS       DAZFVO020000110        17113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFVO020000110 DAZFVO020000000
VERSION     DAZFVO020000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.111"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..97
                     /locus_tag="ACHKNB_RS16910"
                     /old_locus_tag="pgaptmp_003382"
     CDS             <1..97
                     /locus_tag="ACHKNB_RS16910"
                     /old_locus_tag="pgaptmp_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HGU4196836.1"
                     /translation="TTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(157..663)
                     /gene="yciE"
                     /locus_tag="ACHKNB_RS16915"
                     /old_locus_tag="pgaptmp_003383"
     CDS             complement(157..663)
                     /gene="yciE"
                     /locus_tag="ACHKNB_RS16915"
                     /old_locus_tag="pgaptmp_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4196837.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(709..1209)
                     /gene="yciF"
                     /locus_tag="ACHKNB_RS16920"
                     /old_locus_tag="pgaptmp_003384"
     CDS             complement(709..1209)
                     /gene="yciF"
                     /locus_tag="ACHKNB_RS16920"
                     /old_locus_tag="pgaptmp_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HGU4196838.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(1295..1474)
                     /gene="yciG"
                     /locus_tag="ACHKNB_RS16925"
                     /old_locus_tag="pgaptmp_003385"
     CDS             complement(1295..1474)
                     /gene="yciG"
                     /locus_tag="ACHKNB_RS16925"
                     /old_locus_tag="pgaptmp_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HGU4196839.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1855..2661)
                     /gene="trpA"
                     /locus_tag="ACHKNB_RS16930"
                     /old_locus_tag="pgaptmp_003386"
     CDS             complement(1855..2661)
                     /gene="trpA"
                     /locus_tag="ACHKNB_RS16930"
                     /old_locus_tag="pgaptmp_003386"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGU4196840.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(2661..3854)
                     /gene="trpB"
                     /locus_tag="ACHKNB_RS16935"
                     /old_locus_tag="pgaptmp_003387"
     CDS             complement(2661..3854)
                     /gene="trpB"
                     /locus_tag="ACHKNB_RS16935"
                     /old_locus_tag="pgaptmp_003387"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGU4196841.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3866..5227)
                     /gene="trpCF"
                     /locus_tag="ACHKNB_RS16940"
                     /old_locus_tag="pgaptmp_003388"
     CDS             complement(3866..5227)
                     /gene="trpCF"
                     /locus_tag="ACHKNB_RS16940"
                     /old_locus_tag="pgaptmp_003388"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HGU4196842.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGTGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5228..6823)
                     /gene="trpD"
                     /locus_tag="ACHKNB_RS16945"
                     /old_locus_tag="pgaptmp_003389"
     CDS             complement(5228..6823)
                     /gene="trpD"
                     /locus_tag="ACHKNB_RS16945"
                     /old_locus_tag="pgaptmp_003389"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HGU4196843.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6823..8385)
                     /gene="trpE"
                     /locus_tag="ACHKNB_RS16950"
                     /old_locus_tag="pgaptmp_003390"
     CDS             complement(6823..8385)
                     /gene="trpE"
                     /locus_tag="ACHKNB_RS16950"
                     /old_locus_tag="pgaptmp_003390"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGU4196844.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDYLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8407..8500)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8477..8521)
                     /gene="trpL"
                     /locus_tag="ACHKNB_RS16955"
                     /old_locus_tag="pgaptmp_003391"
     CDS             complement(8477..8521)
                     /gene="trpL"
                     /locus_tag="ACHKNB_RS16955"
                     /old_locus_tag="pgaptmp_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HGU4196845.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8659..9540
                     /gene="rnm"
                     /locus_tag="ACHKNB_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             8659..9540
                     /gene="rnm"
                     /locus_tag="ACHKNB_RS16960"
                     /old_locus_tag="pgaptmp_003392"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HGU4196846.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9537..10157
                     /gene="yciO"
                     /locus_tag="ACHKNB_RS16965"
                     /old_locus_tag="pgaptmp_003393"
     CDS             9537..10157
                     /gene="yciO"
                     /locus_tag="ACHKNB_RS16965"
                     /old_locus_tag="pgaptmp_003393"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGU4196847.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10258..11130
                     /gene="rluB"
                     /locus_tag="ACHKNB_RS16970"
                     /old_locus_tag="pgaptmp_003394"
     CDS             10258..11130
                     /gene="rluB"
                     /locus_tag="ACHKNB_RS16970"
                     /old_locus_tag="pgaptmp_003394"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HGU4196848.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11170..11760)
                     /gene="cobO"
                     /locus_tag="ACHKNB_RS16975"
                     /old_locus_tag="pgaptmp_003395"
     CDS             complement(11170..11760)
                     /gene="cobO"
                     /locus_tag="ACHKNB_RS16975"
                     /old_locus_tag="pgaptmp_003395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HGU4196849.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11757..12515)
                     /gene="yciK"
                     /locus_tag="ACHKNB_RS16980"
                     /old_locus_tag="pgaptmp_003396"
     CDS             complement(11757..12515)
                     /gene="yciK"
                     /locus_tag="ACHKNB_RS16980"
                     /old_locus_tag="pgaptmp_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HGU4196850.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDASSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12735..13784
                     /gene="sohB"
                     /locus_tag="ACHKNB_RS16985"
                     /old_locus_tag="pgaptmp_003397"
     CDS             12735..13784
                     /gene="sohB"
                     /locus_tag="ACHKNB_RS16985"
                     /old_locus_tag="pgaptmp_003397"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HGU4196851.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13820..14071)
                     /gene="yciN"
                     /locus_tag="ACHKNB_RS16990"
                     /old_locus_tag="pgaptmp_003398"
     CDS             complement(13820..14071)
                     /gene="yciN"
                     /locus_tag="ACHKNB_RS16990"
                     /old_locus_tag="pgaptmp_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HGU4196852.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14451..17048
                     /gene="topA"
                     /locus_tag="ACHKNB_RS16995"
                     /old_locus_tag="pgaptmp_003399"
     CDS             14451..17048
                     /gene="topA"
                     /locus_tag="ACHKNB_RS16995"
                     /old_locus_tag="pgaptmp_003399"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGU4196853.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKVRYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4176 a         4280 c         4477 g         4180 t
ORIGIN      
        1 taccaccgcc aattataagc taggcggtgc acagcaacac gatagcgtac gcctcgatcc
       61 gtgggtgttt atgttctcag caggatatcg tttttaattc cacaaaaaaa cgaccccgta
      121 atatacgggg tcaataagga catagcataa agtgtattat ttcttcgctt ctacgccgtc
      181 agtttcagag cgaattaaaa atttctcagt tgtttgcgga atatgctgaa tcagccagtc
      241 ggccatttgc ttttcctcat ttaaaatcgc ttcgatggtt ggaattgaag ctgtatcacc
      301 ggcatttttt gctgctgcta atagtgaggt gtaacaggcg atttcaaatt gctcgaagac
      361 atatccgcta atagagcctt tgactatttc atcagaaggg aatataccac cgattgactg
      421 cccaagcgca gccattttac tcatggaatc tttaatgact gaacgtgaaa tgtcattacg
      481 atcaagaata gtttccaatt gaacaatctg gtttttggtt tcactaagat gttgttcaat
      541 acgagcgcgt agttcaggat aattatctat acggctggcc atggattcaa gcatagattc
      601 ggcttgcttt tccattgcgt gggcgtcacg taaccagtca tgataatgtt caatacgatt
      661 catgaaaatg tcctctttat aaaatgacta aaagttaaag ttcatatttc aggctttatt
      721 ttcggctttc ttatttacgt tattaagggc cagatcagtc agtttgatgt cggtggcctt
      781 ttcttcttcc agggtctctt tcagaagctt cgctgcttta cgataaccta attgttcagc
      841 cagcgtcgct aatgtcccgt aactggcaat ctcataatgc tcgactttct gtgctgcggc
      901 aatcagtgcg gcatcacgca cttcgttttt ctcggtactt tcgatgacct cattagcttc
      961 ttcaataaga ccttccattg ccacacattt catgcgctta attttcagat tcgattctga
     1021 ttccacaact tgatcaatac gttcaatctg tccatgtgtt tcctcgaggt gcgcatgaaa
     1081 agcctgactt aatttttcgt ttgatgttgc tcttgcgagt tttgccagtg cccgggttaa
     1141 ttgtttttct gcgctgtagg tatctgaaag caggtgaata aatacatctt caatggtctt
     1201 catattcata tttttctcca gtgaaatcac tgcgagccat acggcccgca gatgcaatta
     1261 tctttatcag ctgatattaa taattaacgg tgaatcagga tttgcctgat ttattaccac
     1321 cgctttgttg accgcctttt ttacccgctt cagatgcgcg ttgcggatca tttttaaaat
     1381 taccgccgct atgctgaccg cctttacggc ctgcgtcgga tgccttctca cggtcttcgg
     1441 cgaaatttcc tgaaccacca cgatgttcgg ccatgttatt tctcccgttg cgttgcattg
     1501 tttcattaat atgagtgttg tgtgtcgaca ctcattaaaa ttagtcgcta atgagaatta
     1561 gtcaaattaa gcgcaacgag aagatagagg gaaaatatat tttgaggaac attctggata
     1621 tattaacaat tacctgagga ataagtgact tagagaaaat tattgatttt actggtgtta
     1681 tgttgcggga ttaatttgtt tgattatatt actgttgggc ggaaaaatga cgtaagttga
     1741 agttcgaccg gggtaagcga aacggtaaaa agataaatat taaatgaatt taggattttt
     1801 ccggcttcat taaagaaagt taaaatgccg ccagcggaac tggcggctgt gggattaact
     1861 gcgcgtcgcc gctttcatcg gttgtacaaa aactttcagt gccgccagca ttttctctgg
     1921 ctcattaata tgttgctcga tgattttaac aatggccgaa ccagaaatcg cgcccgcagc
     1981 tcctgcatca atcgctgctt ttacctgatc cggggcggaa ataccaaacc cctgcaatgg
     2041 aggtgcagcg ttgtactctt tcagcttcgc aaccagatga ttgaggggta acgcggcgcg
     2101 gttttctgcg ccggtcacgc ccgctcgtga cagcaaatag gtgtaaccac gaccgtaaga
     2161 ggctatctgg cgcagcaggt cgtcatcggc atttggcggg cagatgaaga taggtgcgac
     2221 attatggcgc aacgcggcct ggcggaaggg cgcggactct tcaattggca catcggcaac
     2281 cagcaccgaa tcgacgccga ctttctcgca ctgggcataa aactcatcaa tgcctttgtt
     2341 aaacaccaga ttggcataca tcaacaggcc aatgggaatg gtcgggtgtt tctggcgaat
     2401 cagtgccagc atttcaaaac attgtgccgg agtcacacct gccgcaaagg cgcgcagagt
     2461 ggcgttttga atggtcgggc catccgccag tgggtcggag aaggggatac ctaactccag
     2521 cgcgtcagca ccggcttcaa ttagcgtatc gataattttc aatgactgct caatgcccgg
     2581 atcaccgagc gtgacgaaag gaacgaatgc gccttctttg cgctccttca actgggcaaa
     2641 cagagattcg tagcgttcca tcagatttcc cctcgtgctt tcaaaatatc gtgaacggtg
     2701 aagatgtctt tatcgccgcg accggaaagg ttaaccacca gtagctgctc tttatccggg
     2761 ttttcgcgca tcattttcaa cgcatgggcc agggcgtggg aggattccag cgccgggatg
     2821 atcccttcgt gcaggcacag cgttttgaag gcttcaaggg cttcatcatc ggtaatagac
     2881 acgtaatcag cgcgtccagt gctgttaaga tacgcgtgtt gtgggccgac agacgggaaa
     2941 tccagtccgg cggagatgga gtaagattct tcaatctgcc cgtcttcggt ttgcatcatc
     3001 ggcgctttca taccgaaata gatacccacg cgaccatgtt ttagcggtgc gccgtgctcg
     3061 ccagtttcga taccgtgacc acctggctcc acaccaatca ggccgacgtt ggtttcattg
     3121 atgaaatcag caaacatgcc gatggcattc gaaccgccgc caacacaggc gataacggca
     3181 tccggcaggc gaccttctct ttccagaatc tgcgctttgg tttcttcgcc aatcatccgc
     3241 tgaaactcac gcacaatggt cggataagga tgcgggccag ctgcggtgcc cagcatatag
     3301 tgcgcggttt cgtaactacc ggaccagtcg cgcagcgcct cgttacaggc atctttcagc
     3361 gtcgcggaac cgctatgcac cgggatcact tccgcaccca ttaagcgcat acgaaaaacg
     3421 ttaggcgact ggcgttcaac gtctttggca cccatataaa tacggcattt caggccgagc
     3481 agggcgctgg cgagggccga cgccacgcca tgctgaccgg caccggtttc ggcgatgatt
     3541 tcggttttac ccatccgctt cgccagcaac gcctgcccca gcacctggtt agttttatgc
     3601 gcgccgccgt gcagcaaatc ttcacgcttg agatacagcg tggtgttcgt cccggctgta
     3661 atgttctggc atttggtcag cgcggttgga cgcccggcat agtttttcag caggtcgttg
     3721 aactgagcct gaaattcagg atctttttgc gcactgacaa aagcttcttc cagctggcgc
     3781 agagcaggca tcaggatttg tggcacgtac atgccgccaa actcaccaaa ataggggtta
     3841 agtaatgttg tcattgttcc tttccttaat atgcgcgcag cgtctggaaa accgaggcca
     3901 aaagacgtgc gtctttgatg cccggttgcg actctacagc agaattaaaa tcaagtccgg
     3961 cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc taagcccccc gccagcagaa
     4021 cgttgccaag cgattgacca tttaatagtg accagtcaaa acgttgcccg gttccaccct
     4081 ggccgttgtc taaaacatat ttatcaacgt gctgaaactc gcgggcgggc agggtttcac
     4141 cgacgcttaa tgctttccag atggcaacat gtgctggcag agcttcacgc agcgtatcga
     4201 tatacagctg ttcttcatta ccatgcagtt gcactgccgc cagcgataac accttagctt
     4261 tgtccaccac atcggcaata tcgtgattgc ggaacacgcc aacatactgc aacggtgccg
     4321 cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg cggtgatgtc gcaacaaaaa
     4381 tcaacccacc gtaaatcgcg cccgcgtcat aagctgcttt agcatcttgc ccacgcgtca
     4441 ggccacatac tttattctca cccagcaaca cccggcgcac ggcggcgtgc aaatcgtcat
     4501 gggccatcaa cgccgaacca atcagaaaac cgttagcgaa gtggcttaac tcgcgcacct
     4561 gagcgtaagt attgatgccg gattcgctga ttaccgtcac gttgtgcccc agtttcggcg
     4621 caagctcgcg ggtacggttg agatcaatcg acaaatcacg cagatcgcgg ttgttgatgc
     4681 caacgacctt tgcccccaat gcaatggcac gctccagttc ctcttcatta ctgacttcgg
     4741 tcagcacacc catctccaga ctgtgggcga cggcggcaag ctggcgatat tgttcgtcat
     4801 ccagtactga aagcattaat aagcaggcat cagcctggta atagcgcgcc agatagatct
     4861 ggtaaggatc gataatgaag tctttacata aaatcggctg cggggcgatt tggctgacga
     4921 tggggaggaa atcaaagctc ccctgaaaat atttctcatc agtcagcacc gaaattgccg
     4981 aagcgtaatg tttataaatg gcggcaatgc gtgctggatc gaaatcatca cggatcacgc
     5041 cttttgacgg cgacgctttc ttgcactcca gaataaacgc cgtgcgcgca ccctgtagcg
     5101 catcataaaa atgtcgcgtg ctcggctgaa cctcattctg aaaactggcc agcggttgct
     5161 gctgtttgcg ggtttctacc caaatcgcct tgtctgcgac gattttcgct aaaacggttt
     5221 gcatcattta ccctcgtgcc gccagtgcgg taactctgtc gtaagcggaa ccactgcgca
     5281 gtacctcaag aacggtttgc gcattggctt gcagatcttc atggccatgc aggcgcatta
     5341 acatggcgac gttcgcagcg acggctgctt catgggcggc gtcgccttta ccttgtaaca
     5401 agcgtgttaa aatgtcacgg ttttcttccg gtgttccgcc tgccagttgc tcctggtggt
     5461 agggagtcag gccaaaatct tcagcggtca attgatagct cttaatttcg ccgtcatgca
     5521 gctcggcaac gattgtcggc gcgtgtaatg aaacttcatc catcccgccg ctgtgcacca
     5581 ccgccgcgcg ttgatacccc agcacgcgca aggtttcggc aatcggcagc accagttccg
     5641 gactataaac accaattaac gccagcggcg gatgcgccgg gttaatcaat ggccccagca
     5701 cattgaacag ggtgcgggtt ttcagttgct ggcgaaccgg catcgcgtgg cggaatccgg
     5761 tgtgatactt cggcgcaaag aggaaacata cacctaactc atccagcgcc tggcgcgatt
     5821 tatcggcgtt catatcaaga ttaataccga acgccgccag cagatcggac gaaccagatt
     5881 tactggagac gctacggttg ccgtgtttcg ccactttcag cccacaggcc gcggcgacaa
     5941 acgcactggc ggtagaaata ttgatactgt tgctgccgtc accgccagta ccgacgatat
     6001 cagcaaacag ataatccggg cgcgggaacg gcgcggcgtt ttccagtagc gcggttgctg
     6061 ctccggcgat ctcgttcggg tgctcaccgc gaattttcat gctcaccagc gccgccgcca
     6121 gttgttccgg cttcagctcg ccacgcacca ccgctgaaaa cagctggtgg ctttcttgtt
     6181 ggctaagcgt ctgcgcctga tacagttttt ccagaatcgg ttgcagcgtg ttggctggct
     6241 ctagtttctg ctgcgcccag gccagcgttt gttccagcag gcgagcgccc tgggtggtga
     6301 gaatggattc cggatggaac tggaatccac aaacgcgatc cgcatcgtga cgtactgcca
     6361 tcaccatgcc attaaaatgg gcgttgatgg ttaaaccggc cggaatgtta ctgccaacca
     6421 gcgagtgata acgcgccacc ggcagcgggt ttgttaatcc ggcaaacatc gcctgaccgt
     6481 catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc cgcctgaccg acatagcccc
     6541 cgtaagcttc gacaattgcc tgatgtccga ggcaaatgcc aataatgggc agcttgccac
     6601 gcaagcgggt gaggagttcc ggcatacaac cggcttcgct cggcacaccg gggccaggag
     6661 aaagcatcag taccggattg ctcatggtcg ccaggcgttc aattaaggtc tgcgccggaa
     6721 tatggttgcg gtaaatcacc acgttatgcc cattgctgcg caactgatct gccaggttgt
     6781 acgtaaaaga gtcgatatta tcgagcagca gtatgtcagc catcagaaag tctcctgtgc
     6841 atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct ttattacggg tttcgtcggc
     6901 ttccgactgc ggaatagaat caaggactac gccagcaccg gcttgcacgg tggcgatacc
     6961 gttttccacc agcgccgagc ggatcacaat gcaggtgtcg agatcgccgt gcgcggtgaa
     7021 ataacctacc gcgccgccgt agctgccgcg gcgacgacct tccgcctcgg caattaactg
     7081 catggcgcgc actttcggcg caccgcttaa cgtccccata ttcatacagg cgcggtaagc
     7141 gtgtagggcg tcgagatcgt ggcgcagttc accgaccacg cgggagacca ggtgcatcac
     7201 gtaagagtaa cggtcaactt tggtgaggtc ggcgacgtag cggctgccgg gggtgcaaat
     7261 gcgtgccaga tcattacggg caagatccac cagcatcaga tgttcagaaa gctctttatg
     7321 atcggtacgc atttccagtt cgatgcggct gtcgaggtct ctgtccagcg agccatcggc
     7381 acgacgaccg cgtggacgtg ttccggcaat cgggtagatc tcaatctggc ggctggtggc
     7441 gtcatacttg agcgaacttt ccggcgacgc gccaaacagg gtgaaatcat tatcctgcat
     7501 aaaaaacatg tacgggctgg gattactctt tttcagcacg taataggccg ccagcggtga
     7561 cgggcagggc agagagaaac ggcgagatgg caccacctgg aaaatttctc cagcgcgaat
     7621 cgctttttgc aacaaacgca ctacgccacc gaactcttca tcgctctgat tacattcaca
     7681 acgcatatgc ggcacggaaa ccaccggcag cggcggcgcg gcttcggtca gttgctgacg
     7741 tagttcgttc aggcgagcag tgagacgttg tttttcttct tcattcggag caaacaggct
     7801 ggcctgaata cgggtgcttt ttttctgatg gtcaatcacc atcagcgttt cagcgagata
     7861 aaaacagaaa tcagggcagt tattttccgc tgacagttgc ggtaaatctt caaatcccgc
     7921 cacaaggtca taagagaaca ggccgccgaa gaacattgct tctcgttctt ccttcggtac
     7981 attcaacaga ttctgcaata aacggaaagc gtcaaaaacc gaaagggagc ataagcgagc
     8041 gtcttcatcc agcagtggac tgacaggggg gaagcgcagc acacggcagt ttggtaattg
     8101 ttcattttcc acacccgcag gcagggcgtt atccagtagt gtcagcaggg cttcgccgtt
     8161 gccggaaagt gcctggattg tgacagtgtc acctaaagct gtaatgcgca gcgcactgtc
     8221 taccagcagc aggcttttta aataatcttt gctgtcgata tctgcggatt ccagcagcag
     8281 cgttgccgga cgatccccac acaactggtg aaaaagcgcg gtgggattgt cgcgataagc
     8341 gccttcgcag gttagcagtt cgagagtcgg tttttgtgtt tgcattgtta ttctctaatt
     8401 ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat ctgattgctt tacgcatggt
     8461 gaatacactg cccgtttcag gaagtgcgcc accaaccttt cagtacgaaa attgctttca
     8521 ttgtcgatac cctttttacg tgaacttgcg tactagttaa ctagttcgat gattaattgt
     8581 caacagctca tttcagaata tttgccagaa ccgttatgat gtcggcgcaa aaaacattat
     8641 ccagaacggg agtgcgcctt gagcgacacg aattatgcag tgatttacga cctgcacagc
     8701 cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg tgcagtcgag
     8761 atgcgcgtcg gcaccctggc gatcaccgac catgacacca cagctgcaat cgcgccagca
     8821 agagaggaaa tttcacgttc gggactggcg ttgaatctta ttcccggcgt ggaaatttcc
     8881 acggtctggg aaaatcatga aattcatatt gtcgggctga atattgatat tactcatccg
     8941 ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca atcagcgggc gcagctgatt
     9001 gccgaaagac ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcaca acgactggcg
     9061 cagggcggcg cagtgacgcg cggtcatttt gcgcgtttcc ttgttgagtg cggcaaagcc
     9121 agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg atacgttccg
     9181 ccacagtggt gtacaataga acaagctatt gatgtcattc atcattctgg cggtaaggcg
     9241 gtgctagctc atcctgggcg gtacaatctt agtgctaaat ggctgaaaag attggttgcg
     9301 cattttgccg aacaccacgg tgacgcgatg gaagtcgcgc agtgccagca atcgcccaat
     9361 gaacgtaccc agctggcggc ccttgcgcgt cagcatcatt tatgggcatc acaaggatct
     9421 gattttcatc agccatgccc gtggatcgaa ctgggtcgta aactctggct gcccgcaggc
     9481 gttgaaggcg tctggcagct atgggaacag ccgcagaaca ccacagagag ggaattatga
     9541 gccagttttt ttatattcat cctgataacc cacagcaacg tctgatcaac caggcggtgg
     9601 agatcgtgcg taaaggcggg gtgattgttt atccaactga ttccggctat gcgctcggct
     9661 gtaaaattga agataaaaac gcgatggagc gtatttgtcg tattcgccag ctgccggacg
     9721 gtcacaactt taccctgatg tgtcgcgatc tttctgaact ctcgacctat tcatttgttg
     9781 ataacgttgc gtttcgttta atgaaaaaca acacgccggg caactatacc ttcatcttga
     9841 aggggacgaa ggaagtgcca cgccgtctgt tgcaggaaaa acgcaaaacc atcggtatgc
     9901 gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa ccgatgcttt
     9961 ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa gaaattaaag
    10021 accgtctgga aaaacaggtg gatttgatta tccacggcgg ctatctcgga cagaaaccga
    10081 caacggttat tgatcttacc gatgatacgc cggtcgtggt gcgtgaaggc gtaggtgatg
    10141 tgaagccttt cttataagtg cgtaactctg tatactacgc ggccttaaaa tggcgctata
    10201 cggcgccatt gattctcctg ttcgacgcct gtgaaggcga caccaaaagg aagctctatg
    10261 agcgaaaaat tacagaaagt gctggcccgc gctggccacg gctcccgccg tgaaatcgaa
    10321 tctattattg aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact cggcgatcgc
    10381 gttgaagtta cccctgggct gaaaattcgt atcgatggtc atctgatttc ggtacgtgag
    10441 tccgctgaac aaatttgtcg cgttctggcc tattacaaac cggaaggtga gttgtgtacc
    10501 cgtaacgatc cggaagggcg tccgacggtg tttgaccgtc tgccaaagtt gcgtggcgca
    10561 cgctggattg ccgtaggtcg tctggacgtt aacacctgtg gtctgctgct gttcaccacc
    10621 gatggtgaac tggcaaaccg tttaatgcat ccaagccgtg aagttgaacg tgaatacgcc
    10681 gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg atttgagtcg tggcgtgcaa
    10741 ctggaagatg gtccggcagc ttttaaaacc atcaagttca gcggcggcga agggatcaac
    10801 cagtggtaca acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg tctgtgggaa
    10861 gcggttggtg tgcaggtgag ccgcctgatc cgtgttcgtt acggtgatat cccactgcca
    10921 aaaggtctgc cacgcggtgg ctggacggag ctggacctcg cccagactaa ctatctgcgc
    10981 gaactggtgg agcttccgcc agaaaccagc tctaaagtcg ctgtagaaaa agaccgtcgt
    11041 cgcatgaagg ctaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt gagcggcggt
    11101 cgtcgttctg gcgggcgtaa taacggttaa tcaaagtatg ccggacgtca tatccggcat
    11161 ttttacagat taataatcaa tccctatctg cgcttttaca ccggcatcaa acgcatgttt
    11221 gacggggcgt aattcactta ccgtatctgc cagttcaaga atatcccgat gacaaccacg
    11281 acccgtgata atcaccgtct gttgatgtgg acgttcattt aacgcctgca ccacttcttc
    11341 cagtggcaaa tagtcatacg ccaccatata cgtcagttca tcaagcaaaa ccatatccag
    11401 tgaggaatca gcaagcatcc gctttgcatg ttgccagact tcgcggcagg cggcggtatc
    11461 agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc
    11521 atgtggctcc agcagattgc gttcgccatt aggccaggtg cctttaataa actgcacgac
    11581 gcctactttt tttccgtgac caactgcgcg tgttgccgta ccaaatgccg cggtagtttt
    11641 gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg cctgggccac
    11701 acgagcatct actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat
    11761 tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg
    11821 ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc
    11881 tgtggatctt cggtcgggaa ggcgcttgcg cgcattgcgg tgcgcgtacc gcctgggtta
    11941 atgcagttga cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg
    12001 gtggcaaatt tcgacgctgc atatgcaccc cagttagctc gtccctgacg tccaacgctt
    12061 gatgaagtaa agaccagtga actggcgtcc gatttgagta ataaaggaag cagtgcctgg
    12121 gtgagcataa aggtggcatt aacgttgacc tgcatgacgt cctgccagac ctgcggattt
    12181 tgttcgctca ttgggcaaat atcgccgagc aatccggcat tatgcaaaac accatccaga
    12241 cgcggataat taacgacaat gcgctgtgcc agttgttggc aattttcgga cgtgcaggtc
    12301 agcaaatcga gaataaacca ctgtggctga cgcccagttt cttcgtttat gtggctggct
    12361 acctgacgta atttttcttc attacggccc aacagaatca ctgtcgcacc atagcgtgca
    12421 tacgtcatcg cggcttcacg accaatccca tcgctggctc ccgtcaccag gataatgcga
    12481 tcattgagta aatcttgttt tggctggtaa tgcatggcta ctcctcaacg acgttgtctg
    12541 tcgcgctgcc agtgtacttt atgactttgg ggctttatgc ctgaaacaga agggcatttc
    12601 aatcagcctg gggcgataac accgcaaaat ggatactttg tcatactttc gctgcaataa
    12661 catctctgcg agacggctta acatgcctgt tgtaaactgt gagccaaagc gttgtttaac
    12721 caaggtgggg actcgtggaa ttgttgtctg aatatggttt gtttttggcg aaaatcgtta
    12781 ccgttgtgct agcgattgcg gcgattgccg ccattattgt caatgttgct caacgtaata
    12841 aacgccagcg tggcgagtta cgggtcaaca atctcagcga acagtataag gagatgaaag
    12901 aagaactggc cgcggcgctg atggactcac atcagcaaaa acagtggcac aaagcgcaga
    12961 agaaaaagca caagcaagaa gcgaaagcag caaaagcgaa agccaaactg ggcgaggtgg
    13021 caactgacag taaacctcgc gtctgggtgc tggattttaa aggcagcatg gacgcccatg
    13081 aagtgaactc gctacgtgaa gagataacgg ctgtactcgc agcattcaaa ccgcaggatc
    13141 aggttgtgct acgtctggaa agccctggtg gcatggtgca tggttacggg ttggcggctt
    13201 cgcagctgca gcgtctgcgt gataaaaaca ttcctttaac tgttacggta gacaaagtcg
    13261 ctgccagcgg cggttacatg atggcctgtg tggcggacaa aattgtttcc gcaccgtttg
    13321 ctattgtggg ttccattggc gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa
    13381 gcaaagatat tgatatcgaa ctgcacactg ccgggcagta taagcgtact ctgaccttgc
    13441 tgggtgaaaa taccgaagaa gggcgggaga aattccgcga agagttgaac gaaacgcatc
    13501 agttgtttaa agattttgtg aagcgtatgc gtccgtctct ggatattgaa caggtggcaa
    13561 cgggtgaaca ctggtacgga caacaggcgg tagagaaagg cctggttgat gaaatcaaca
    13621 ccagtgatga agttattctt agcctgatgg aaggccgtga agtggttaat gtacgctata
    13681 tgcagcgtaa acgactcatt gaccgattca ccggcagcgc ggcagagagc gccgatcgat
    13741 tgttgctacg ctggtggcag cggggtcaaa agccattgat gtaaaagaca aacgcgaggc
    13801 taagacctcg cgttttgctt taatcaacca gatgatattt ttctgaaagc acatgggcca
    13861 ggtgtttgaa catattaaac accgcggtgc ttttggctgt tggcaatcct tgttcatcta
    13921 aaaagtagtc gccggtaaat accagcacgc cattacgctg cgtgacgccg gtggcttcaa
    13981 tccctgcgag cgtatcctca tgctcacgaa tgattttgtt ggcctctttc aacagcgttt
    14041 cgcggtcgat gggttgtgtc tctttgttca ttatttactc cttaaacaag gacattagtc
    14101 tacgccaggc atggcttgca gacaaatata ccacgctggt ggcaagagcg ccttactggt
    14161 aactttggat tttgcatgct aataaagttg cgtatcggat tttatcaggt acagtgtgac
    14221 gctttcgtca atctggcaat agatttgctt gacattcgac caaaattccg tcgtgctata
    14281 gcgcctgtag gccaagacct gttaactcag tcacctgaat tttcgtgaac agagtcacga
    14341 caaggggttg atatccgcag agaacgagtc catatcggta actcgttgcc agtggaaggt
    14401 ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg taaggtgaat atgggtaaag
    14461 ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg
    14521 actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt ggctcagctg
    14581 ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa aaggatgaac
    14641 gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag gcgcactatg
    14701 aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa acaactggct gaaaaagccg
    14761 accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca tggcacctgc
    14821 gggaagtgat tgggggtgat gatgcgcgct atagccgagt ggtgtttaac gaaattacta
    14881 aaaacgcgat ccgccaggca tttaacaaac cgggtgagct gaatattgat cgtgttaatg
    14941 cccagcaggc gcgtcgcttt atggaccgcg tggttgggta tatggtttcg ccgctgctat
    15001 ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg gttcgcctgg
    15061 tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg gaagtcgatg
    15121 ccagcacgac cacgccatct ggtgaagcgt tggcgttaca ggtgactcat cagaacgaca
    15181 aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag
    15241 tgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt
    15301 ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc gtgaaaaaaa
    15361 ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg cgtaccgact
    15421 ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc gataattttg
    15481 gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac tcacaggaag
    15541 cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg aaggatatgg
    15601 aagcagatgc gcagaaactg taccagttaa tctggcgtca gttcgtagcc tgccagatga
    15661 ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc cgcctgaaag
    15721 cacgcggtcg tattttgcgt tttgatggct ggacaaaagt gatgcctgcg ttgcgtaaag
    15781 gcgatgaaga tcgcatctta ccagcagtta ataaaggcga tgctctgacg ctcgttgaac
    15841 ttacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca tcgctggtta
    15901 aagagctgga aaaacgtggt atcggtcgtc cgtctaccta tgcgtcgatc atttcgacca
    15961 ttcaggatcg tggctacgtg cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg
    16021 aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac gactttaccg
    16081 cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg aaagctgtac
    16141 tggatcactt cttctcggat ttcacccagc agttagataa agctgaaaaa gatccggaag
    16201 agggtggtat gcgcccgaac cagatggttc tgaccagcat tgactgcccg acttgtggtc
    16261 gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct ggctatgcgc
    16321 tgccgccgaa agagcgttgc aaaaccacca ttaacctggt gccggaaaac gaagtgctga
    16381 acgtactgga aggcgaagac gctgaaacca acgcgctgcg cgcaaaacgt cgttgcccga
    16441 aatgcggcac ggcgatggac agctatctca tcgatccgaa acgtaagttg catgtctgtg
    16501 gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc attaaaggtt
    16561 atgacggccc gatcgttgag tgtgaaaaat gtggctctga aatgcacctg aaaatggggc
    16621 gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa aaacacacgt aagattttac
    16681 gtaacggcga agtggcacca ccgaaagaag atccggtgcc attacctgag ctgccgtgcg
    16741 aaaaatcaga tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc ctggctgcca
    16801 acactttccc gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt tatcgcttcc
    16861 gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc gccacagcag gatccggaag
    16921 gtaataagac catggttcgc tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa
    16981 aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg gttgaaggaa
    17041 aaaaataacc tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa gcggcccttt
    17101 tttattgcat att
//
LOCUS       DAZFVO020000111        16789 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFVO020000111 DAZFVO020000000
VERSION     DAZFVO020000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.112"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(126..278)
                     /locus_tag="ACHKNB_RS17000"
                     /old_locus_tag="pgaptmp_003400"
     CDS             complement(126..278)
                     /locus_tag="ACHKNB_RS17000"
                     /old_locus_tag="pgaptmp_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4196854.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(543..1277)
                     /gene="cysH"
                     /locus_tag="ACHKNB_RS17005"
                     /old_locus_tag="pgaptmp_003401"
     CDS             complement(543..1277)
                     /gene="cysH"
                     /locus_tag="ACHKNB_RS17005"
                     /old_locus_tag="pgaptmp_003401"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HGU4196855.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1351..3063)
                     /gene="cysI"
                     /locus_tag="ACHKNB_RS17010"
                     /old_locus_tag="pgaptmp_003402"
     CDS             complement(1351..3063)
                     /gene="cysI"
                     /locus_tag="ACHKNB_RS17010"
                     /old_locus_tag="pgaptmp_003402"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HGU4196856.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3063..4862)
                     /gene="cysJ"
                     /locus_tag="ACHKNB_RS17015"
                     /old_locus_tag="pgaptmp_003403"
     CDS             complement(3063..4862)
                     /gene="cysJ"
                     /locus_tag="ACHKNB_RS17015"
                     /old_locus_tag="pgaptmp_003403"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HGU4196857.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5181..5543
                     /gene="queD"
                     /locus_tag="ACHKNB_RS17020"
                     /old_locus_tag="pgaptmp_003404"
     CDS             5181..5543
                     /gene="queD"
                     /locus_tag="ACHKNB_RS17020"
                     /old_locus_tag="pgaptmp_003404"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HGU4196858.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5621..6892
                     /gene="ygcN"
                     /locus_tag="ACHKNB_RS17025"
                     /old_locus_tag="pgaptmp_003405"
     CDS             5621..6892
                     /gene="ygcN"
                     /locus_tag="ACHKNB_RS17025"
                     /old_locus_tag="pgaptmp_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGU4196859.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6883..7143
                     /gene="ygcO"
                     /locus_tag="ACHKNB_RS17030"
                     /old_locus_tag="pgaptmp_003406"
     CDS             6883..7143
                     /gene="ygcO"
                     /locus_tag="ACHKNB_RS17030"
                     /old_locus_tag="pgaptmp_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HGU4196860.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7160..7735
                     /gene="ygcP"
                     /locus_tag="ACHKNB_RS17035"
                     /old_locus_tag="pgaptmp_003407"
     CDS             7160..7735
                     /gene="ygcP"
                     /locus_tag="ACHKNB_RS17035"
                     /old_locus_tag="pgaptmp_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HGU4196861.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7883..8743)
                     /gene="ygcQ"
                     /locus_tag="ACHKNB_RS17040"
                     /old_locus_tag="pgaptmp_003408"
     CDS             complement(7883..8743)
                     /gene="ygcQ"
                     /locus_tag="ACHKNB_RS17040"
                     /old_locus_tag="pgaptmp_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HGU4196862.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8740..9519)
                     /gene="ygcR"
                     /locus_tag="ACHKNB_RS17045"
                     /old_locus_tag="pgaptmp_003409"
     CDS             complement(8740..9519)
                     /gene="ygcR"
                     /locus_tag="ACHKNB_RS17045"
                     /old_locus_tag="pgaptmp_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HGU4196863.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9497..10906)
                     /gene="ygcS"
                     /locus_tag="ACHKNB_RS17050"
                     /old_locus_tag="pgaptmp_003410"
     CDS             complement(9497..10906)
                     /gene="ygcS"
                     /locus_tag="ACHKNB_RS17050"
                     /old_locus_tag="pgaptmp_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4196864.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10928..12382)
                     /gene="ygcU"
                     /locus_tag="ACHKNB_RS17055"
                     /old_locus_tag="pgaptmp_003411"
     CDS             complement(10928..12382)
                     /gene="ygcU"
                     /locus_tag="ACHKNB_RS17055"
                     /old_locus_tag="pgaptmp_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HGU4196865.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12452..13237)
                     /gene="ygcW"
                     /locus_tag="ACHKNB_RS17060"
                     /old_locus_tag="pgaptmp_003412"
     CDS             complement(12452..13237)
                     /gene="ygcW"
                     /locus_tag="ACHKNB_RS17060"
                     /old_locus_tag="pgaptmp_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HGU4196866.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13556..14833
                     /gene="yqcE"
                     /locus_tag="ACHKNB_RS17065"
                     /old_locus_tag="pgaptmp_003413"
     CDS             13556..14833
                     /gene="yqcE"
                     /locus_tag="ACHKNB_RS17065"
                     /old_locus_tag="pgaptmp_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HGU4196867.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSLVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14860..16338
                     /gene="ygcE"
                     /locus_tag="ACHKNB_RS17070"
                     /old_locus_tag="pgaptmp_003414"
     CDS             14860..16338
                     /gene="ygcE"
                     /locus_tag="ACHKNB_RS17070"
                     /old_locus_tag="pgaptmp_003414"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HGU4196868.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGLITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLSESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4049 a         4387 c         4199 g         4154 t
ORIGIN      
        1 catccgtaat atatatgaat aataaacata ggcataatat tcacataaac aatataaatt
       61 aaaaaagggt gcaggagacc aaaccatcac aattcagtac gcggggtttt acccccgcgt
      121 ggtggctact tcttcgattc gtaagcgaga actgccttaa actccatacc acgttctctg
      181 atactcaact cacacaacga gtcgcctacc atcagcgtga atcccagtac tgtaaaacac
      241 agcactatga ttgccacaag ggcatatttt gtcagcatgt cttgcctccc tgcaaagaag
      301 agactaacat tcgaattgtt tgggttcgaa cgtaagcctc aggttgatag aaatatcgcc
      361 tggggctttt gtccatctgg aacctcgcga atgcttaacg ccagacagcc tcaagcaccc
      421 gacgccattc tatacctgat aattcttcca gcggcgttca cgtttaaaat caggaatact
      481 gctcgcaaaa taaaaacgcc cccatcattt ctgacagagg cgtttaattt atccggcaat
      541 atttaccctt cgtgcaatcc acattccctt tttaagccaa agaaacgtgt ttcttcttcc
      601 gacatgccag gttcccattt acgggttgta tgggtatcac cgaccgataa atatccttca
      661 tcccataatg ggtgatattt caggccatgt ttttgcaggt actgataaat agttcggtta
      721 tcccagtcga taatcggcag cactttaaat acgccacgct gaattgccag caccggtaaa
      781 ttggcgcgac tgccggattg ttcacggcgc aggccagcaa accaggtttg cgcgttaagt
      841 tctttcagag cccggttcat cggttcgact ttgttgatgt cattgtactt ttcaatgcct
      901 tcaacgccct gttcccacag tttgccgtag cgtgcttcct gccaggcggc gctttcggta
      961 gcgcggtaca ctttcaggtt gagcttgagt ttgtccgtta actcgtcaat aaagcggtag
     1021 gtttccggga acagatagcc ggtgtcggtg aggatcaccg gaatatccgg gtgaatctga
     1081 ttcaccagat gcaggctcac cgccgcctga atgccaaagc tggaagaaag tacatattca
     1141 ccgggcagat tatccagcgc ccaggcaacg cggccttcag cgtccagttt ttccagttcg
     1201 gcgttagttt ctgccagcgc cagaatgcga tctaccttcg gcagttcgtt cagggcgttt
     1261 agatcgagtt tggacataga ttcctcactg tttgccttgc ctgatgcgac gctcacgcgt
     1321 cttatcaggc ctacaagacc gggctgatgg ttaatcccac aaatcgcgcg ccggatcgag
     1381 caccgggcga atgatgcccg cacgcaccgt aaagtcgccg aagccttcac ccgcttcgcg
     1441 ctctttcgcc cagcgcccta tcagttcatc aagcgacgcc aggatttccg gctcggtgat
     1501 gttttcttta tacatccgtg ggatacgtgt cccaatgcgg ttgccgccaa gatgcaggtt
     1561 gtagcgaccc ggcgctttac ccactaggcc cacttccgcc agcatcgcgc gaccacaacc
     1621 gttcgggcag cctgttacac gcatcacgat atgctcatcg ctgacaccat gtttcgccat
     1681 taaattatcg atgttgtcga taaaagacgg caggaaacgc tctgcttccg ccatcgccag
     1741 cgggcaagtc gggaacgaga cgcaggccat cgagttttca cgctgcggcg tgacggcatt
     1801 cattaacccg ctctctttgg cgatcttctc gatcttcgct ttctcgcttt ccggtacacc
     1861 ggcgatgatc agattctggt tcgccgtaat gcggaaatcg cctttgtgga tcttcgcgat
     1921 ctccagcagg ccggttttca gcggacgccc cggataatca aggatgcgac cattttcgat
     1981 aaacagcgtc aggtgccagt tatcatcaat gcccttaacc cagccaatac gatcgcctcg
     2041 tccggtgaac tcatatggac ggatcggttc aaatttgatc cccgcgcgac gctccacttc
     2101 cgctttaaac gtctcaaccc ccacgcgctc cagcgtgtat ttggttttgg catttttacg
     2161 atcggttcgg ttaccccagt cacgctgagt tgtcacgacg gcttcggcca ccgccagcgt
     2221 atgctccagc ggcagatagc caaactcgct cgccgtgcgg gcgtaggttt tcttgttgcc
     2281 gtgttcgata gaaagcccac cgcccaccag caggttgaag cctaccagct tgccgttttc
     2341 ggcgattgcc acgaagttca tgtcgttggc atgcagatcg atatcgttct gcggcgggat
     2401 cactaccgtg gttttgaact tacgcggcag gtaggtctga ccaaggatcg gttcttcatc
     2461 agtagtggcg actttttcgt ggtcgagcca gatctccgca tacgcgcggg tacgaggcaa
     2521 cagatgctca gaaatcttct tcgcccactc gtacgcttcc gcgtgcagct gcgactcgta
     2581 agggttcgag gtgcagagta cgttacggtt catgtcgtta gctgtcgcca gcgcatcaag
     2641 accgaccgag tgcagcatct ggtgcaccgg tttgacgttc tttttcagaa tgccgtggaa
     2701 ctgaaacgtc tggcggttgg tcaggcgaat gctgccatag atggtgtttt caccggcaaa
     2761 tttgtcgatc gcctgccact gtttagtggt aatcacccca cccggcagac gacagcgaag
     2821 cagcatcgcg tggcgcggct ccagcttctg ttcagcacgt tcggcgcgga tgtcgcggtc
     2881 atcctgctga tacatgccgt ggaagcggat cagcaggaag ttgtcgccct taaagccgcc
     2941 ggtcagaccg tcgtttaaat cttccgcaat ggtgccgcgc aggtagttgc tttcaagctt
     3001 catgcgctcg gcgtctgtca gttttccttc gaccactaaa ggccctggat gtttttcgct
     3061 cattagtaga catctcgctg ataacggcgc tctacgcgca gctcacttaa aaattcatcc
     3121 gccgcttcgg tgtccatgcc accaaattcg gcaatcactt ccagaagtgc ctgctcaacg
     3181 tctttcgcca tgcgattagc gtcgccgcag acataaatgt gggcaccatc attgatccag
     3241 cgccacagct ccgcgccctg ttcgcgcagt ttgtcttgta cgtaaacttt ttctttttgg
     3301 tcgcgcgacc aggcaagatc gatacgtgtc agcacgccat ctttgacgta gcgctgccac
     3361 tccacctgat acaggaagtc ttccgtaaag tgcggattac caaagaacag ccagttttta
     3421 cctggcgctt cgtcggcggc gcgttgctgc ataaaggcgc ggaacggcgc aataccggtg
     3481 cctgggccaa tcataatcac cggggtttct ggattggctg gtaggcggaa gttatcgtta
     3541 tgttcgataa atacgcggac ttcgccctct tcttccacgc ggtcagcgag gaagctggag
     3601 gcaccaccgg cacgggcgcg gccttccacg tcgtaacgca ccacaccaac ggtgacgtgt
     3661 acttcgttct cgacttccgc ctgcgaggag gcaatggaat agagacgcgg cgtcagcggg
     3721 cgcagcagat taattagcgc ttcggcatca agctgtgccg gggagaaacg caccatgtcg
     3781 acaatcggcg tcgtcgcggc gtaatgctgt aacttcgctt tatcgcccac cagcggcagc
     3841 agtgtttcgc tgcgggtaag cgtggcgtaa ttctcaacaa tgttggcggt gttgacggtc
     3901 agttcgaagt gccactgtag cgcttcgttc agaggcaacg ttttgccctc gacggtgaca
     3961 ggttcatcgc ctttcagcca cagcagttcg acaagttctt tcaccagtgc cggatcgttc
     4021 tgataccaga cgcccagcgc gtcacccggc tggtaacgca ggcccgagtc acctaagtca
     4081 atttcgatat ggcgaacgtc tttttcagag ttacgcccgg taattttctg gttaacagag
     4141 aggctagcca ccagcggcgc gtctttgctg tacgggctgg tgtggatttc atttaccgcg
     4201 ccagtagcga cggattgcga aggtgccgcg acaggcgcac gcgatttaag cgcatcaacc
     4261 acgcgggcgc gccactcgct ggcagcagcc tggtattcaa catcggcatc gacacggtcg
     4321 agcaggcgtt caccacccag ttccgccagc ttgctgtcaa aatctttccc ggactggcag
     4381 aaaaattcat aagagctatc gccgaggcta aacacggcaa atgcggtgtt ttccagcttc
     4441 ggcgcttttt tggagaacag gaacttatgc agcgcgacgg cttcttccgg cggttcccct
     4501 tctccttgcg ttgacgtcac cacgataagc agtttttcgc tggcgatttg tttgaattta
     4561 tagtcgcccg cgttcaccag cttaacgttc agttttgccg ctaataaatc atcacgtaat
     4621 gcttcagcaa cacggcgcgc attgccggtt tgcgaggcgg agataatagt tatacccggc
     4681 atttctgcgg cgggagctgg cgtcgctgca agcgcagcag gctgctgatt gagcacgccc
     4741 cagaaatagc cagaaaccca ggcaagctgg gtgggagtta aatcggtcgt ggccgcctga
     4801 aggcgtacca gttgctccgg gttcaacgga agcaacgcgg aaggtgggac ctgtgtcgtc
     4861 atgcgtcgtt atgttccagt aagcaaagct gtttctgcgc cctgtcagcg cccataaaac
     4921 agaagagaag gtaaggttaa cggggtgaac ggtgtggatt aaagacggga tagcgataac
     4981 taataaccaa atcgactaac ctgttttagc aatagtcttt aacaacaaaa tagattaacc
     5041 aacctaatga aaaacaaatg aatttagcca atcattaaga taaatcagcg attttgcgca
     5101 acaaagtcgt tttagataat gcgaaaaaac agcctttccg gtactctacg gcggttttat
     5161 cgtcctgtag agaaattatg atgtccacca cgttatttaa agatttcacc ttcgaagccg
     5221 ctcaccgctt accacacgtc ccggaagggc ataaatgtgg tcgcctgcac gggcattcct
     5281 ttatggtgcg actggaaatt accggggaag tcgatccgca tacgggctgg attatcgatt
     5341 tcgctgaact gaaagcagcg tttaagccaa cttatgagcg cctcgatcac cattatctca
     5401 atgatattcc aggcctggaa aacccgacca gcgaagtttt agcaaaatgg atttgggatc
     5461 aggttaaacc cgttgtgccg ctgttaagtg cggtgatggt aaaagaaacc tgcaccgctg
     5521 gttgtatcta tcgcggcgaa tgataagagt gtgtcggcgg tcaatttccc ttaagtaacg
     5581 ctatgttagg gtgttgtgtt ctggatatct ggggcatgac atggaagacg actgcgacat
     5641 tattattatt ggtgccggta ttgcaggcac cgcttgcgcg ttacgctgcg cgcgagcggg
     5701 tttatccgtt ttgttactgg aacgcgctga aatccccggc agcaaaaatc tttccggcgg
     5761 gcggttatat acccatgcac tcgcggaact cctccctcag tttcatctga ccgcgcctct
     5821 tgaacgatgc atcactcacg aaagcctttc cctgttaacg ccggatggcg caacgacgtt
     5881 ttccagctta cagcccggcg gtgaatcctg gagtgtatta cgtgcacggt tcgatccgtg
     5941 gctggttgcc gaagccgaaa aagaaggtgt cgaatgcatc cctggtgcga cggtggatgc
     6001 actgtatgaa gaaaacggca gggtgtgtgg tgtcatttgt ggtgacgata ttctccgcgc
     6061 ccgttatgtg gtgctggcag aaggtgccaa cagcgtcctg gctgagcgtc acgggttagt
     6121 gactcgtcct gctggcgaag cgatggcgtt ggggatcaaa gaagtgctgt cgctggaaac
     6181 atccgctatt gaagaacgtt ttcatctgga gaataacgaa ggcgcagcgt tgctgttcag
     6241 cggcgggatc tgtgatgact tacccggcgg cgcatttctt tatactaatc aacaaacgct
     6301 ctcgttaggg attgtttgcc cgctctcttc ccttacgcaa agccgtgttc cggcaagcga
     6361 gctgctgact cgctttaaag cgcatccggc agtgcgcccg cttatcaaaa acacggaatc
     6421 actggagtat ggtgcgcatc tggtgccaga aggtggcttg cacagtatgc cggtgcaata
     6481 cgccggtaac ggctggctgc tggtgggcga tgcgttgcgc agttgcgtca ataccggaat
     6541 ttccgtgcgc ggcatggata tggcgctgac tggcgcgcag gcggcggcac aaacgctgat
     6601 aagcgcctgc cagcaccgcg agccgcaaaa tctgtttccg ctttatcatc acaacgtaga
     6661 gcgcagcctg ctgtgggatg ttctacagcg ttatcagcat gttccggcgc ttttgcaacg
     6721 cccgggatgg taccgtacgt ggcctgcgtt aatgcaggat atttcccgcg atttatggga
     6781 tcagggtgat aaacctgttc caccgctgcg ccagttattc tggcatcatt tacgtcgtca
     6841 cggcctgtgg catctggcgg gcgatgttat caggagtctg cgatgtctgt agcccgtaat
     6901 ctctggcgcg ttgctgatgc gccgcacatt gttccggctg actccgttga gcgccagacg
     6961 gcagaacggt tgattagcgc ctgtccggca ggtctttttt cgctcacacc ggaaggtgac
     7021 ttacgtattg actatcgcag ttgcctggag tgtggcacct gccgtttgct gtgcgacgaa
     7081 tcaacactac aacagtggcg ctatccgcct tccggattcg gcatcaccta ccgctttgga
     7141 taaataataa ggataattta tgcccctctt acacctgctc cgccagaatc cggtgattgc
     7201 tgccgttaaa gataatgcca gcctgcaact ggcaatcgat tctgaatgcc aatttatttc
     7261 cgtgttgtac ggcaatatct gcaccatcag taatatcgtt aaaaagatta agaacgccgg
     7321 gaaatatgct tttattcatg ttgatttgct ggaaggcgcg tcaaataaag aagtcgttat
     7381 tcagtttttg aaactggtga ccgaggcgga cggcattatc agtaccaaag cctcaatgtt
     7441 gaaagcggca agagcggaag gttttttctg tattcatcgc ctgtttattg ttgattcaat
     7501 ttcgtttcac aacattgata agcaagttgc gcaatcgaat ccggattgta ttgagatcct
     7561 gccaggctgt atgcccaaag tgctgggctg ggtgacagag aaaatccgcc aaccgctgat
     7621 tgccggtggg ctggtgtgcg atgaagaaga tgcgcgtaat gcgattaacg cgggtgtcgt
     7681 ggcgctttcc accacgaata ccggggtctg gacgttagcg aaaaaattac tttgacggga
     7741 taatcgtaac caattgaatt tggtttgatt tgtaggccgc acgccacatc cgacattcag
     7801 cgcctgatgc gacgcttgac gcgtcttatc aggcctacaa gtccccaggc cagaaccgta
     7861 ggcaggatca tccggcaaca cgttattggc aatctgcgtg aatgtttgtc accagtgcct
     7921 ccagcaccac tttccagtca tcaaccacgc caacatctgc ctgtgaaaat accgcagcgc
     7981 tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg
     8041 ctgcgccaga agcgccaaca acaatacaca cctcaggtgc cagcagatgt ccggaaatac
     8101 caatcacttt ttcagcatca acgccaccgt tcatcactcg cgcccgactg tagcccactt
     8161 cggctcccag cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc
     8221 ccaccaccag cacccgtctg gcctcagcta atgggtcgcg agtaacattt ttcagatcct
     8281 ctgtgctaac cagccagtcc ggaagcgcac ccgggacaat gttcagttgt tgcattccag
     8341 agggcaacgt ggcgtttttc gctgctcccg cctgacgcgc cagggaaagg catagcgggc
     8401 gtttttctgt ttgcagcgtc gccgttagcg catttcccca gtgggatttg cgcacgctta
     8461 cggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca
     8521 gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg tccgcaggcg
     8581 ttcgctgcca ctgttccacc aacgcatcca gcacctgttc cgccactaca ggctgaggtt
     8641 caatctgcca gtgcgccagg gtacaaccgc tgaaatcctg cgccgccagc cagcttgcta
     8701 ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca tcctttggcg
     8761 taaatagtcc cgccatagct tttgagcttt ttctgctacc gtttgcccat caatcagcgt
     8821 cgctccgcgt cgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcatttcggc
     8881 agcaacagtt ttacggataa tttctgcttt ccccgcagcc attcgctggc gcattcccgg
     8941 tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg
     9001 gcaacaacgc aacccatgtt cagtacgctg ttcgagggtg ataaacagcg cgtcgagagt
     9061 gaaacgctcc acctgggtga agcagggcca gcccagcatt tccgccagca aaaatggcgt
     9121 ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca gcggattctg
     9181 atgctgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc
     9241 cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca gccagtgcaa
     9301 tgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt tttttctctg
     9361 cgccaacagc agcgcggcag cggcctgttc atcagcaccg agtaaacttc gcagtagcga
     9421 aatatccggt ccgcttttac cctgagccgc cgcctgccac tctttttccg ccagcattcc
     9481 ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct acatttcccg
     9541 ccgccaccag cgggagggct ttagtttctg gtgcccatag ccaggtcaca acaaaaccaa
     9601 ccaacaacac tgtcgccagg agcaataagg tgacttgcat tccccactgc gccagcaccc
     9661 acggcagcag gccagtactt accgccgcgc caagtcgact catggcggtg gcaaaaccga
     9721 cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc accagattac
     9781 tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat gaactgccgg
     9841 aaggcaaaca ggccattact accagcgttg ccgccagcag caaaaaactt cccagcaaaa
     9901 atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc acaattaaca
     9961 acgcattaag catcaggctg gcagtcagcg catcttccag accaatagtc tgggcgatag
    10021 ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag aagacgctgt
    10081 taaacgccgt gcgccgccag taacgcgaag agaacaaggt tttgatgtgt ttatgggtcg
    10141 ccgttaccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc acgatagcgt
    10201 gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg tgattctggc gttccccagc
    10261 gtaataacgt aatcaacaac gcgggcagag ctgccgatgc cagtagccag cgccaggcct
    10321 ccgggttttc ggaaataaag tgatgtccgg caatacttgc cagcacatag cctacggtcc
    10381 acaccacgct gaatgcgccc agcaaaatac cgcgatggcg gcgcggggaa aattcagcca
    10441 gcaaggtgtg acctactgaa taatcgcctc ccagaccaat gccaatcaaa atgcgcagtc
    10501 caataagatg ctctggcgtg gtggcaaaaa attgtaaaaa cgaagcaagc gtaatcagca
    10561 aaaagctgaa ggtgaagatt ttttgccgac caatatggtc ggagatccat ccaagaacca
    10621 ggctgccaag gaacaaacca aggagcgccg agccgccgat cattcccgcc ataaacggcg
    10681 tcagttgcat ggcgggcgta agctgaataa tggcgtaacc aatgacgccg agaacataac
    10741 cgtcggtcag gtgtgcgccg aaagtgagcg cagcaatgcg gcagtgaaaa cggttaagcg
    10801 gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg tccggtaata
    10861 agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc cgtcatcgcg gaacgctgcc
    10921 tgacacatta tttttcaatc ggatagatag tacccgtatt cataatgcca ttaggatcga
    10981 actgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagt ttgctccagt
    11041 gaacgcgatg tttaccgata ccgtggtggt gcaccatcga accgccgagg cgaatggttt
    11101 cttcacagat gatcttgttg agcggattgt ggtacttgtc gatttcctct tccggcttac
    11161 agtcaacgac gttgtaatcg tagacgaagt acatgttggt gccgttctga tagctatgag
    11221 aggaatgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcagta cgaatacggt
    11281 taataacgct ttcgtagatt tcgtggatgc agctccagca gccggacact tcagtggtaa
    11341 agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta tccggtcccc
    11401 agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg cattgcgggt
    11461 agcgggcaac gatttccgca atcccttcgc ccgtcgcctt cgcaatgcga gggttacctt
    11521 cagccataaa gatcagcacg cattttccgt ctgcaaaatg ggtgaagtgt tgggtgccat
    11581 cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg acttcacgca
    11641 ggatgttgaa gccggttttc atgtcttcca ggatatagcc gtagaagagg ttgttttccg
    11701 gggtgaattt aaagattttc actgttactt cagtgatata gcaaagtgca ccttcgttac
    11761 cgatgatgat gtggcgaatg tccgggccag ccgcgcggcg tggcacgttt ttaatgcgtg
    11821 tgacggtgcc atcagccaat actgcttcca gaccaacgac catatcttcg attgcgccgt
    11881 agagtgtgga gaactgcccg atactgcggg ttgctaccag gccgcccatc tgagccagcg
    11941 gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgca ttttccagca
    12001 cctccagcgg aacgccacat tgcgccgtcg cctgcatatt ctcaatatca atattaatga
    12061 tttgattcat ggcggagccg tcgagcacca ccgagttttc cacaacggtt tccagcccac
    12121 cttcagtggc ggaagcgccg gtacgcggca caccgttaat tttgtgcgca ttcataaaat
    12181 tcagcacacg ggatacttgc tctgtggaac cgagttttac gaccgctgcc ggaatcggca
    12241 aagtataaat gccatgaata tccggaaatt tacgaaaacg gtcaatactg ttcttcttta
    12301 atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagt tggtcgacaa
    12361 tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg aataatcatt
    12421 aataagagaa aacagaaacc accgttatgg attagcgcac taaataacca ccatcgacca
    12481 ctaataaatg cccattgaca taatttgaag ccggacttgc gaggaataca gctgcgccca
    12541 ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg cgctgattgg
    12601 tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca ggggcaatac
    12661 catttacctg aatattatat tgacctagtt catcacaata agctttggtg aatccggcaa
    12721 gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa gagaacaatg
    12781 aacagatatt aataattttg ccgcttttct gcgggatcat aatttttgca gcttcatagc
    12841 ttaactcgaa tgcggcggtc aggttcacat caatcatcgg atcccagtcg gcacgaccga
    12901 agtccagcac cttattcagc ttacaaatac cggcattgtt taccagaata tcaactgtac
    12961 cgaaacgctc acagcaagca gcgataatct tctgcggcgc gccttctgcg gtgatatcca
    13021 cctgcatgaa gtccacctca acaccctgtt tttcaatcat ttcctttgtt tcgccgttat
    13081 ctttgacgaa actaggaata aagatatttg cgccagcttt ggccaacgcc atggcaaatg
    13141 cctggcctaa accgctattc ccaccggtaa caattgcggt tttacctttc agtgagaaaa
    13201 aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat ttccgtaagg
    13261 caaaaaaaaa gaaaggtaac ttcccccgcg agagggaagt tacctttctc attatcgctg
    13321 gtaactggag ataattttta gcataacggc ataactgcta atgtgatcgt aatcacagtg
    13381 tgatattcgt attgtcaaat agttttaaaa agcagaagcg tgattactct cacacaacac
    13441 ctctgctttc catgttattt cttattgcaa ttactagaga cctgagaaaa gtaatccacc
    13501 tgaaagggcg gcttactttt ctcttttttt tgtctattac aaatgggaat acgatatgca
    13561 acacaactca tatcgccgtt ggataaccct cgcgataatt agttttagcg gcggcgttag
    13621 ttttgacctg gcttatttac gttatattta tcaaattccc atggcgaaat ttatgggatt
    13681 cagcaatacc gagataggtt taataatgag tacctttggt attgcggcta ttattcttta
    13741 tgcccccagc ggcgttattg ccgataaatt ttcacaccgc aaaatgatta cttccgcgat
    13801 gatcattacc ggattactgg gtctgttaat ggcaacgtat ccaccgctgt gggtaatgct
    13861 ctgtattcag gtcgcctttg cgataacgac gattttaatg ctgtggtcgg tgtcgattaa
    13921 agccgcctcg ttgctgggcg atcatagtga gcaagggaaa attatgggct ggatggaagg
    13981 gctgcgcggc gtcggtgtaa tgtcgctggc ggtgtttacc atgtgggtct tttctcgctt
    14041 tgcaccggat gacagcgcca gcctgaaaac agtcattatc atctacagtt tggtttacat
    14101 cttgttgggg attctgtgct ggttttttgt tagcgataac aacaacctgc gcagtgccaa
    14161 taacgaagaa aaacagtcat tccagcttag cgacatcctt gccgttttgc gtatcagcac
    14221 cacctggtat tgcagcatgg tgatttttgg tgtcttcacc atctacgcca ttctgagtta
    14281 ctccaccaac tatctgaccg aaatgtacgg catgtcgctg gtggcggcga gctacatggg
    14341 gattgtgatc aacaaaatct tccgcgcgct gtgcggccca cttggcggca ttatcaccac
    14401 ctacagtaaa gtgaaatccc ctacccgcgt gatccaaatc ctttccatca tcggtttgct
    14461 ggcgttgaca gccctgctcg tcacgaactc taacccgcaa tcggtcgcga tggggattgg
    14521 cctgatttta ctgctgggat tcacctgtta cgcctcacgc gggctgtact gggcctgccc
    14581 cggcgaagcg agaacaccgt cttacattat gggcaccacg gtaggtattt gttcggtgat
    14641 tggattcctg ccggatgtct tcgtttaccc gattatcggc cactggcaag acaccctgcc
    14701 cgctgcagaa gcctaccgca atatgtggct gatgggcatg gcggcgctcg ccatggtgat
    14761 tgtctttacc tttttgctgt tccaaaaaat tcgtactgct gatagcgccc ccgcaatggc
    14821 tagcagcaag taagccagga aaattacagg agaaaaatca tgtcgaaaaa atacatcata
    14881 gggattgatg gcggaagtca gagcacaaaa gtggtgatgt acgatctgga aggtaatgtg
    14941 gtttgcgaag gtaaaggctt attacagccg atgcacacgc cttatgccga tactgcagaa
    15001 catcctgacg acgatttatg ggcatcatta tgttttgcag gtcacgattt gatgagccag
    15061 tttgccggga acaaagaaga tattgttggc atcggcctgg gctccatccg ttgctgccgt
    15121 gcgttattga aagccgacgg cacgccagct gcgccgttga ttagctggca ggatgcacgc
    15181 gttacacgcc cttacgaaca tacgaatcct gacgtggcgt atgtcacctc tttttcgggt
    15241 tatctgacgc atcgcttaac cggcgagttt aaagacaata tcgccaacta ttttggtcag
    15301 tggccggtgg attataagag ctgggcatgg agcgaagatg ctgcggtaat ggataagttt
    15361 aatatccccc gtcatatgct gtttgatgtg caaatgcctg gcaccgtcct cggacttatc
    15421 acaccacaag ccgcactggc gacacatttc ccggcaggac tgccggttgt ttgtaccacc
    15481 agtgataaac cggtagaagc tctgggggcc ggattactgg atgatgaaac tgcggtaatt
    15541 tctcttggca cttacatcgc attgatgatg aacggcaaag cactgccgaa agatccggtg
    15601 gcgtactggc cgattatgtc ttctattccg caaacattgc tgtatgaagg ttacggtatt
    15661 cgcaaaggta tgtggacggt gagctggctg cgtgacatgt taagcgagtc gttaattcag
    15721 gatgccaggg cgcaggatct ttcaccggaa gatttactca acaagaaagc ctctagtgtg
    15781 ccacctggct gtaatgggct gatgacggtg ctggactggc tgaccaatcc gtgggaaccg
    15841 tacaaacgcg ggattatgat cggctttgat tccagcatgg attacgcatg gatatatcgt
    15901 tcgatattgg aaagcgtggc gctaacgctg aagaacaatt acgacaatat gtgtaatgaa
    15961 atgaatcact ttgcgaagca tgtgatcatt actggcggcg gttcgaacag cgatctgttt
    16021 atgcaaattt ttgccgacgt gttcaacctt ccggcacgcc gtaacgccat taacggttgt
    16081 gcaagtctgg gagcagcgat taatacagcg gtaggtctgg ggctataccc ggattacgca
    16141 acggctgttg ataagatggt tcgcgtgaaa gatatcttta taccgattga gagcaatgcc
    16201 aaacgctacg acgcgatgaa taaaggcatt ttcaaagacc taaccaaaca tactgatgtg
    16261 atcctgaaaa aatcgtatga agtgatgcat ggggaattgg ggaatgcgga ttcgatccag
    16321 agctggtcga atgcgtgaat aaggtaggcc gggcggaaaa ctgcccggcc tgaacatacc
    16381 tgaatggtta tccccgctga cgcggggaac ataagttcga caacttaaaa aatgataaca
    16441 agtccggttt atccccgctg atgcagggaa cataataaaa actttaacta gttcggttta
    16501 tccctgtttt tacaaggaat attaactaca ctcgatgtct ttagaataaa agacaatacg
    16561 aatcgcactt tatcaatttt cacttaaagt atgaactgga tatctttatt cataagaact
    16621 attcatcaac agcgtataga ggcggttatg taaaaccact cattagcctc aaaacaaaga
    16681 caaacttcca gatttattac ttaaccaatg aataacaagc ttaaaaaata tactgttatt
    16741 ctaaagaaaa aattaacagg cattaattca atattaaaaa aataataga
//
LOCUS       DAZFVO020000112        16635 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFVO020000112 DAZFVO020000000
VERSION     DAZFVO020000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.113"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(418..1614)
                     /gene="hemY"
                     /locus_tag="ACHKNB_RS17075"
                     /old_locus_tag="pgaptmp_003415"
     CDS             complement(418..1614)
                     /gene="hemY"
                     /locus_tag="ACHKNB_RS17075"
                     /old_locus_tag="pgaptmp_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HGU4196869.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1617..2834)
                     /gene="hemX"
                     /locus_tag="ACHKNB_RS17080"
                     /old_locus_tag="pgaptmp_003416"
     CDS             complement(1617..2834)
                     /gene="hemX"
                     /locus_tag="ACHKNB_RS17080"
                     /old_locus_tag="pgaptmp_003416"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HGU4196870.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(2856..3596)
                     /gene="hemD"
                     /locus_tag="ACHKNB_RS17085"
                     /old_locus_tag="pgaptmp_003417"
     CDS             complement(2856..3596)
                     /gene="hemD"
                     /locus_tag="ACHKNB_RS17085"
                     /old_locus_tag="pgaptmp_003417"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HGU4196871.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3593..4534)
                     /gene="hemC"
                     /locus_tag="ACHKNB_RS17090"
                     /old_locus_tag="pgaptmp_003418"
     CDS             complement(3593..4534)
                     /gene="hemC"
                     /locus_tag="ACHKNB_RS17090"
                     /old_locus_tag="pgaptmp_003418"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HGU4196872.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4921..7467
                     /gene="cyaA"
                     /locus_tag="ACHKNB_RS17095"
                     /old_locus_tag="pgaptmp_003419"
     CDS             4921..7467
                     /gene="cyaA"
                     /locus_tag="ACHKNB_RS17095"
                     /old_locus_tag="pgaptmp_003419"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HGU4196873.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7507..7827)
                     /gene="cyaY"
                     /locus_tag="ACHKNB_RS17100"
                     /old_locus_tag="pgaptmp_003420"
     CDS             complement(7507..7827)
                     /gene="cyaY"
                     /locus_tag="ACHKNB_RS17100"
                     /old_locus_tag="pgaptmp_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HGU4196874.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7989..8131)
                     /locus_tag="ACHKNB_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /pseudo
     CDS             complement(7989..8131)
                     /locus_tag="ACHKNB_RS17105"
                     /old_locus_tag="pgaptmp_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8290..8493
                     /gene="yifL"
                     /locus_tag="ACHKNB_RS17110"
                     /old_locus_tag="pgaptmp_003422"
     CDS             8290..8493
                     /gene="yifL"
                     /locus_tag="ACHKNB_RS17110"
                     /old_locus_tag="pgaptmp_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HGU4196875.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8530..9354
                     /gene="dapF"
                     /locus_tag="ACHKNB_RS17115"
                     /old_locus_tag="pgaptmp_003423"
     CDS             8530..9354
                     /gene="dapF"
                     /locus_tag="ACHKNB_RS17115"
                     /old_locus_tag="pgaptmp_003423"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HGU4196876.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9351..10058
                     /gene="yigA"
                     /locus_tag="ACHKNB_RS17120"
                     /old_locus_tag="pgaptmp_003424"
     CDS             9351..10058
                     /gene="yigA"
                     /locus_tag="ACHKNB_RS17120"
                     /old_locus_tag="pgaptmp_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HGU4196877.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10055..10951
                     /gene="xerC"
                     /locus_tag="ACHKNB_RS17125"
                     /old_locus_tag="pgaptmp_003425"
     CDS             10055..10951
                     /gene="xerC"
                     /locus_tag="ACHKNB_RS17125"
                     /old_locus_tag="pgaptmp_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HGU4196878.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTVVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10951..11667
                     /gene="yigB"
                     /locus_tag="ACHKNB_RS17130"
                     /old_locus_tag="pgaptmp_003426"
     CDS             10951..11667
                     /gene="yigB"
                     /locus_tag="ACHKNB_RS17130"
                     /old_locus_tag="pgaptmp_003426"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HGU4196879.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11751..13913
                     /gene="uvrD"
                     /locus_tag="ACHKNB_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11751..13913
                     /gene="uvrD"
                     /locus_tag="ACHKNB_RS17135"
                     /old_locus_tag="pgaptmp_003427"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HGU4196880.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPAEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14060..14824)
                     /gene="yigE"
                     /locus_tag="ACHKNB_RS17140"
                     /old_locus_tag="pgaptmp_003428"
     CDS             complement(14060..14824)
                     /gene="yigE"
                     /locus_tag="ACHKNB_RS17140"
                     /old_locus_tag="pgaptmp_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HGU4196881.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            14986..15042
                     /gene="ysgD"
                     /locus_tag="ACHKNB_RS17145"
                     /old_locus_tag="pgaptmp_003429"
     CDS             14986..15042
                     /gene="ysgD"
                     /locus_tag="ACHKNB_RS17145"
                     /old_locus_tag="pgaptmp_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HGU4196882.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            15194..16144
                     /gene="corA"
                     /locus_tag="ACHKNB_RS17150"
                     /old_locus_tag="pgaptmp_003430"
     CDS             15194..16144
                     /gene="corA"
                     /locus_tag="ACHKNB_RS17150"
                     /old_locus_tag="pgaptmp_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HGU4196883.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(16198..>16635)
                     /locus_tag="ACHKNB_RS17155"
                     /old_locus_tag="pgaptmp_003431"
     CDS             complement(16198..>16635)
                     /locus_tag="ACHKNB_RS17155"
                     /old_locus_tag="pgaptmp_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HGU4196884.1"
                     /translation="LCCIYNLWTLRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWY
                     LKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIV
                     KNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
BASE COUNT         3816 a         4311 c         4443 g         4065 t
ORIGIN      
        1 attgaatgaa ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt
       61 cactcaggaa gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg
      121 ttaagggatg ttatttcccg attctctgtg gcataataaa cgggtagatg ctcattccat
      181 ctcttatgtt cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc
      241 ttatcgtcca ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag
      301 tgaagcaccc acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag
      361 gcgttttttt atgcatcaga aaggtcccgg aggtgcttgc ctccgggtga gaaggaacta
      421 ctgtggtggg ttgttctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc
      481 ttccggcttg tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg
      541 acgttgtttc agcgccgcgc ggaaggcgag cgatgcttcc tgccattctc cgtgcttcat
      601 cagtgactgg cccagtgtgc tccacaacag cgggcgatcg ccgacgtttt tgatctgctg
      661 gcgcagcact ttttccagct gttccggatt gttggttttc agtcgaggaa tcggcagcag
      721 taggcgatcg tcgtactggc gtttcaggcc atcgatgata atttgctggg cagtatcatg
      781 atcgtcacat tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt
      841 tttccggctt tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc
      901 ctgatccatc aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc
      961 aacatgggct ttcgccattg atgggatgat atccagcaga gaactccatg cacctgtgcg
     1021 gatatacgcc tgttccgcca gacgtaatac ttccggatgg cgtggcgtaa cttccagcag
     1081 cttatccacg ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg
     1141 ggtgatttct accggaatgg tgtcattgcc ggccagctct gctgcgcgtt ccagatgttg
     1201 gttggcgcgt gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac
     1261 caccggttgt tccgcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc
     1321 gccttccgcc agtttcagca gcgcctgttc ggtctgctta cgggcacggc gacgcttacg
     1381 tccgacaaac cacccacggg tgtgtgcgcc tgtgcggaag atccgccgca gtagccactc
     1441 aatggcaaac agcactacca tcgccagaat caaaataatc gccaggcccg tgacgctggt
     1501 ttcgatattg tagttgtcag tctggatcag cacgtaaccc tgatgaccgg caatcatcgg
     1561 gccaaccact atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat
     1621 tctccttgcg gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca
     1681 ggtgcaggtg caggtgcagg tgcaggttta gcttccgttg tccccgctgc cggttgtgcc
     1741 agcaggttac gcacgcgggt ctgcatcagt ttttccagca tcgcctggct ttgcagggtt
     1801 tccggaagat ccatcgagat attttgctgg cttaactggt ccacctcgtc gaggaacgct
     1861 ttggtggtgg catcatcagt atcgtagtaa gctcgtaccc aggtggagac gttctccagc
     1921 gcctggcgat aagtctcttc ctggtgacgc ggtacagctt gtgctgcgac cagcaggcga
     1981 gagcgaatat tttcgcgcag atagatatcc tgatttggcg ctaacagcgg tacggcggtg
     2041 tcatcacgac ggcgaatcgt aatgaagttg tccataaagt tctgccagct tttttgcaga
     2101 ttgatacgcc attcgctgat ggaactggaa agctcttcac cgtcggaatc catcggcgaa
     2161 ccatcgctgt cattatcggc cagacgcagg ttatctacct gatttgaaag ctgattaagc
     2221 ttaaggatga tgccgtcata atccacctgc gaaactgcag aaaggctggc gatatcatcg
     2281 gtaattgccc gacgaacggt aatcagactc gggtcattca tatccgccag gctggcatct
     2341 gcacttttca gcaacgctgc agcggtcgtg acgtcctgat cgctccacag cttccgtccg
     2401 gcgagtttca ccagaaaatc ggcctgagcc agcagccagg ttttagcatc gctgccggaa
     2461 atggtggcga ccttttgttg gacttcatcc aactgttttg ccagcgtttc ttgctgacga
     2521 ttcgcctgct taagctgtgc agcttgttgc ttaataatgc cttccagctc ggctttttgg
     2581 ctctcctggg ctttttgcaa tgccgtcagt tggttagcca gggcatcgct ggtggcggtc
     2641 tgattgacgg cctgttgttt accccagcca tacaaaccga cgcccgccgc cagagcaata
     2701 gcgatagcca ccgcgctgag aatcaatgcg gtattgttct tactcttttt ttctgttgcg
     2761 acaggttgtg acgtggtgtc cacggcctcc ctggtctctt caaccacggc ggaggttttt
     2821 tcttgttccg tcattatggc ttcctgttat gagagttatt gtaatgcccg taaaagcgca
     2881 tcgttgtcag cgttatcggc gaccttaatg tcttgccagc ccagttcccg ggcgagtttc
     2941 gccaaacgct cactgacgac caatagtcga cagtgtagta accagtgctc acgataccat
     3001 tgtgggatca gcgaccagag ttgctgcaac atttcaccgc tggtaacaac gaccatcgtc
     3061 acctcgcggg attgccagcg catcgcttct tctgcaccat cgtaatggat tgcgcatcgt
     3121 tgataacatt cacaaaaagt gacctcagca ccgcgcgctg tcagggtatc cccaattagc
     3181 tcacgaccgc cattgccacg taatatcagc gcacgtttgc ccgcaatatt ttgtaattca
     3241 ggtaattgta gcaagacttc gctgatttcc cgatcctgcg ggtagagaat cttctgtccg
     3301 cttacggtat gtagcgccag tgcggtggtg cgtccaatgg cgaaataatc aggtagtcgg
     3361 ggccatttac gatcttgctg atgcagctgt gattgggcaa aagcaaccgc gtgttgcgag
     3421 agggcaaaca acagatcgct ctcccccagc gccgccagtt gatcagcaag ttgcggtaat
     3481 tgtcgacccg gagaaaactc aatcagcgga aaatgccagg ccacctgccc cagtgtgcgc
     3541 agacggctca ctaactcttc tccagcggga gacgggcggg tgaccaggat actcatgccg
     3601 gggcgtctcc gttatagact tcagcgagga tctcgcgcgc gccgttattc agtagctctt
     3661 ctgccagcga aatccccatt tgttcggcat tttgcggcgc accgcggcgt tcaccgcgaa
     3721 taatctgcga accgtctggc gcgccgacca gcgcacgcag ccagatttca ccatcaataa
     3781 gctcggcgta gctaccaatt ggcacctgac atccaccttc gagacgggta ttcatggcgc
     3841 gttctgcggt aacgcgcagt gcagtttcgt ggtgattcag cgcggcaagc agctcgcgag
     3901 tgcgcgtatc atcaaggcgg cattcaatac cgactgcacc ttgtcctacc gccggaagag
     3961 aaatctcggg tggtaacgcg gcgcgaatgc gggactccag acctaaacgt tttagtccgg
     4021 ctacggcaag aatgatggca tcgtattcgc cgttatctag tttgctcagg cgagtgccga
     4081 cgttgccgcg cagggagcgg ataatcagat ccgggcggcg ttcagccagt tggcactggc
     4141 gacgtaaact ggacgtcccg acgatactgc ctgccggtaa cgcatccaga ctgtcataat
     4201 tattggacac aaaggcatcg cgaggatctt cacgctcaca aatagtgacc aaccccagac
     4261 cttgcgggaa ttcaaccggc acatctttca ttgaatgtac ggcgatatcg gcgcgattct
     4321 ccaggagcgc gacttccagc tcttttacaa ataagccttt tccgcctact ttcgccagcg
     4381 gcgtatcaag aatcacatcg ccgcgcgtca ccatcggtac cagttcaacc accaggcccg
     4441 gatggctcgc catcaacttg tctttgacat agtgtgcctg ccagagtgca agtgggcttt
     4501 ggcgtgtggc aattcttaaa acattgtcta acatgcttgt taccgtcatt atcatccgtg
     4561 gtccatccta acatccttgc cagagtgatg tcagtgttgt ggtgaaacgt agacgcctgc
     4621 gcaaaccgta aaatgaggtc tggcagtgga tcctgacagg cgtttcacgc cgtcgtaata
     4681 aggaatttac agagaataaa cggtgctaca cttgtatgta gcgcatcttt ctttacggtc
     4741 aatcagcaag gtgttaaatt gatcacgttt tagaccattt tttcgtcgtg aaactaaaaa
     4801 aaccaggcgc gaaaagtggt aacggttacc tttgacatac gaaatatccc gaatgccgcg
     4861 tgttaccgtt gatgttggcg gaatcacagt catgacgggt agcaaatcag gcgatacgtc
     4921 ttgtacctct atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg
     4981 gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca
     5041 ttgttgcact atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc
     5101 atttgccttt acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat
     5161 cgtggaatgt cagtacagga cccaccgaaa ggtgagcttc caattactgg tgtatacacc
     5221 atgggcagca cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt
     5281 catcaatcct ggctcgatag cgaagagcgc cagttactac aacgtaaatg tagtctgctg
     5341 gaaagctggg ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc
     5401 ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata
     5461 ctgctgcttg acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg
     5521 aatatggtgc cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg
     5581 cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct
     5641 gaagagtact ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa
     5701 gcggtactga aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg
     5761 ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggtctcgat
     5821 ccatactgca tgatgctgga gcgtgttact gaatacctga cggcgattga agattttacc
     5881 cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaagct cagccgtgaa
     5941 cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg
     6001 gacgaagctc gtctggcaat gctcgataac cgcgctaact ggaagattga tcaggtgcgt
     6061 gaggcgcaca acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt
     6121 gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt
     6181 aagctgtatg ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt
     6241 tcacccgatc tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgtgctaac
     6301 cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag
     6361 ccgctggaat ataaccgtta cctgaacaaa ctggtggcgt gggcatggtt taacggcctg
     6421 ctgacctcgc gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg
     6481 caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg
     6541 aaggcgctct acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat
     6601 gacccgacag cggcgttccg caatcaggtg gtgcattttg atttccgtaa gctggatgtc
     6661 ttcagctttg gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac
     6721 tcgtggaacg aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg
     6781 aaaactattc tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc
     6841 tgttatagcc agcatctgcg cgggttaatc cgtactcgcg tgcagcaact ggtttctgag
     6901 tgtattgaac tgcgcctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc
     6961 gtttctggtc aaacctgggg gttgttcttc gaacgcctga atgtatcggt acaaaaactg
     7021 gaaaacgcca tcgagtttta tggcgctatt tcgcataaca aactgcacgg cttgtcagtg
     7081 caggttgaaa ccaatcacgt caaattaccg gcggtggtgg acggctttgc tagtgaaggg
     7141 atcatccagt tcttcttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc
     7201 gacgaaagca accgggttga ggtgtatcac cactgcgaag gcagcaaaga ggaactggtg
     7261 cgtgacgtca gtcgcttcta ctcgtcatcg cacgaccgct tcacctacgg ctcaagcttc
     7321 atcaacttca acctgccgca gttctatcag attgtgaagg ttgatggtcg tgaacaggtg
     7381 attccgttcc gcacaaaatc tatcggtaac atgccgcctg ccaatcagga tcacgatacg
     7441 ccgctattac agcaatattt ttcgtgatga acgtgccgga aagcgaggct tatccggcat
     7501 gcaatcttag cggaaactga ctgtttcacc cgcttgctgt gtcgccgcct gttccagcaa
     7561 atcccagaag gtttcgccgc tgcgatcgca aatccattca tcgcctttta ggtcaaaatg
     7621 gtagccgccc tgtttggttg ccagccacac ctggtgcagc ggctcctggc ggttaataat
     7681 gattttgctg ccgttttcaa aggtaatggt cagtacgccg ccgttgattt cgcagtcgat
     7741 atcgctgtcg ccatcccagt cgtccaggcg ttcttcaatg gtcagccaga gttgatcagc
     7801 caggcgatga aattcactgt cgttcattgt tgtatcctgt ttttaagtga tggcggcagt
     7861 atagcggcat ggggtcaggg cttcaaagtt tgcacctctg cggctgcgtt ccggcacgat
     7921 tcatccgtca ccggaataat gatgtctcag tgtagcgaaa gatttgtctc ttcattaggg
     7981 cgcagttaca ccacgccttt ccctgtttct ggtaaacatt atgatcaggt ttaccgagcg
     8041 agcatcctca cgctgacgga actaaaaaag ccaacaaaca aactacattg cgatagtgca
     8101 taaagccatc ctggcgcgag gtgccgatca cgaaactacc agcaaaacat aaatccccac
     8161 gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag agtcgctaaa
     8221 cgcttgcttt tacgtcttct cctgcgatga tagaaagcag aaagcgatga actttacagg
     8281 caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct cttcagcctg
     8341 acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa cgcaccgccg
     8401 ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa cgatcgcgcc
     8461 actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc cgcgtgattg
     8521 gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat ggtcgtcgac
     8581 gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc tgatcggcac
     8641 ctgggggtag ggtttgacca actgctggtg gttgagccgc cgtatgatcc tgaactggat
     8701 tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg caacggtgcg
     8761 cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga tatccgcgtc
     8821 agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt ccgcgtaaat
     8881 atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa agcggaaaag
     8941 acctatatta tgcgcgccgc cgagcagaca atcttatgcg gtgtggtgtc gatgggaaat
     9001 ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac gcttggtcct
     9061 gttctggaaa gccacgagcg ttttccggag cgcgccaata tcggttttat gcaagtggtt
     9121 aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac ccaggcctgc
     9181 ggcagcggcg cgtgtgcggc ggttgcagta gggattcagc aaggtttgct ggccgaagaa
     9241 gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc gggtcacccg
     9301 ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct atgaagcaac
     9361 caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt gtcgattatc
     9421 tgattaaaaa tcctgagttt tttatccgta atgcgcgcgc agtagaagcg atacgtgtgc
     9481 cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc gcacgtaatc
     9541 atattcatgt tctggaagag aacatggcgc tgttgatgga acaggctatc gccaacgaag
     9601 gcctgtttta tcgcctactc tacctgcagc gcagtctcac cgccgccagc agtctcgacg
     9661 atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggcaggt gcgagtctgc
     9721 gcctgtttcc ggatcgctgg cgcttaggtg cgccgtcgaa ccacactcat ctggcattaa
     9781 gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag cactatcttg
     9841 ggccgcttaa cggacctgag ctgctggtgg tgctacctga agcgaaagca gtgggatcgg
     9901 tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt accagtcgcg
     9961 atgccagtca ctatcaacaa gggcaaggaa cgcagttact tcatgaaatt gcgctgatgt
    10021 tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca ccgatgtaga
    10081 acgctaccta cgttatctga gcgtggagcg ccagcttagc ccgataaccc tgcttaacta
    10141 ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc aaagctggca
    10201 gcaatgtgat gtgacggtgg tgcgcaattt tgctgtacgc agtcgccgta aagggctggg
    10261 agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact ggctggtcag
    10321 ccagaacgaa ctcaaagcta acccggcgaa aggtgtttcg gcaccgaaag cgccgcgtca
    10381 tctgccgaaa aacatcgacg tcgacgatat gaatcggctg ctggatattg atatcaatga
    10441 tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg gtctgcgtct
    10501 ttctgagctg gtggggctgg atattaaaca cctcgacctg gagtctggtg aagtgtgggt
    10561 tatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg tggcgtggat
    10621 tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc tttttctgtc
    10681 gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg aatggggcat
    10741 aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt tcgctacgca
    10801 tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc atgccaacct
    10861 ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg tgtacgatgc
    10921 ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt ggggcgcatc
    10981 tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt gattttgcgc
    11041 accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg cagcttccag
    11101 aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga gatttatcac
    11161 gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc cgggctgagt
    11221 gccgaagaag ccagtgcagg cgcacacgca gcaatgatca actttgccaa atggcgcagc
    11281 cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa gaaatggccg
    11341 ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg ggattatttt
    11401 gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga tatgtacttt
    11461 ctggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg ggacgatctc
    11521 accactgacg tgggtggggc aattcgcagc ggaatgcagg cctgttggat cagaccggaa
    11581 aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cgcatctgga aatttcccgg
    11641 ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc agctttttgg
    11701 cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca atggacgttt
    11761 cttacctgct cgacagcctt aatgacaaac agcgcgaagc ggtggccgcg ccacgcagca
    11821 accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg catcgtatcg
    11881 cctggctgat gagcgtggaa aactgctcgc catactcgat tatggcggtg acgtttacca
    11941 acaaagcggc ggcggagatg cgtcatcgta tcgggcaact gatgggcacc agccagggcg
    12001 gcatgtgggt cggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg caccatatgg
    12061 acgccaatct gccgcaggat ttccagatcc tcgatagcga ggaccagctg cgcctgctta
    12121 agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc caggcaatgt
    12181 ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa agctacggta
    12241 atccggcgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg tgtgaccgcg
    12301 cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg cttaacaagc
    12361 cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac gaattccagg
    12421 ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc ggcaaagtga
    12481 tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag gtggagaata
    12541 ttcagcgttt ccttaatgat ttccccggtg ccgaaactat tcgtctggag caaaactacc
    12601 gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat aacgggcgtc
    12661 tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc tattgcgctt
    12721 ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg caggacaacg
    12781 gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg cgtgtactgg
    12841 aagaagcact gttgcaggcc agtatgccgt accgtattta cggcgggatg cgcttcttcg
    12901 aacgccagga aatcaaagac gcgctctcgt atctgcgcct gattgccaac cgtaacgacg
    12961 acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac cggacgctgg
    13021 acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca tgtcgtgaac
    13081 tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcga tttatggaat
    13141 taatcgatgc cttagcgcaa gaaactgccg atatgccgct gcatgtacag actgaccggg
    13201 taattaaaga ctccggcctg cgcaccatgt acgagcagga gaagggcgaa aaaggccaga
    13261 cgcgtatcga aaacttagag gaactggtga cggcaacgcg ccagttcagc tacaacgaag
    13321 aagatgaaga tttaatgccg ctgcaggcat tcctctccca tgcggcgctg gaagcgggcg
    13381 aggggcaggc ggatacctgg caggatgcag tgcagttgat gacgctgcac tcggcgaaag
    13441 gcctggagtt cccgcaggtg tttattgtcg gtatggaaga gggtatgttc ccaagccaga
    13501 tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac gttggtgtaa
    13561 cccgcgcgat gcagaaactg acgctgacct acgcggaaac ccgccgtctg tatggtaaag
    13621 aggtttacca tcgcccgtcg cgctttatcg gcgagctgcc ggaagagtgt gtggaagagg
    13681 tgcgcctgcg cgctacggta agtcgtccgg tcagccatca acggatgggg acgccgatgg
    13741 tcgagaacga cagcggctac aagctcggtc agcgtgtacg ccacgctaag tttggtgaag
    13801 gcaccatcgt caatatggaa ggcagcggcg agcatagccg tttgcaggtg gcattccagg
    13861 gccagggtat taaatggctg gtggcggcat acgcccggct ggagtcggtg taacgttgcc
    13921 ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcatattcaa taaattgcaa
    13981 cgtcatgtag gccggatagg gcgtttacgc cgcatccggc atctgcgcca tcttcagtat
    14041 ctgacacaaa actatcgttt taacctttcc gctctacgga aatcatggtg acaaaaggat
    14101 agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg ccatcaagat
    14161 aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca taaaaatttg
    14221 tcgcctgctg gctcaacaaa aacacggcat tcccatgttt attaatccca acaccgttac
    14281 gaattttacg tgaggcgacg ttgggatgaa tacgcggatt aattacactg ttttccatca
    14341 acattggccc tgactgcacc gcaaactgaa tctctttact ggttttgaag gcatccagac
    14401 gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg ataaagaaat
    14461 tcccttcacc tgaagcgaga tttaacgcca ccttctgctg accgttttcg atgtacaaac
    14521 cgagcggcgc atagctttca tcatagatgc cgccgttcat cgccatctgc acctgaccct
    14581 gactattcat atccgccagc agagcatgta aagttcccca cgcttcgcca ttggcttttt
    14641 gccagtacat tttcacccgc tctgtttgag gattaacggt atacgcctgt acggtcagcg
    14701 tcggatctga gagtgcgcaa tcatcagcgg caacagcaaa caagggaagt aaggtgaggg
    14761 cgagaaaaat ccgtttgaga ttcaaggtga tcattccttt accaatgagt agccgatgcg
    14821 ccattatagg tcctggatgt tggatttttt tatcctgtta gcgaccttga cgagtaccaa
    14881 aaagcgcgaa gttcaactat tgttctgtgg tgttctgttg cgtgttgacg gcaaaatttt
    14941 gctggcgtaa catgcgcgca cgatcactct aagaggacat tcgccttgga cacacccagt
    15001 agatactggc tcactatcct gtcatccagg atcaactcct aaggctatcc ctttttgctg
    15061 atagccttag cggttgtcag cgacctcaat ttttcccgtc gcgctgagtc aggctgttta
    15121 atggtctgaa acccaatttg tttctgtgtg cccaccgaac tgtccgatat tttaagcatt
    15181 gggagtcccg gtcatgctga gcgcatttca actggaaaat aaccgactga cccggctgga
    15241 agtcgaagag tcacaacccc ttgtaaatgc agtatggatt gatcttgtcg aaccggacga
    15301 cgacgagcga ctgcgcgtac aatctgaact tggccagagc ctggcaaccc gcccggaact
    15361 ggaagacatc gaagcatcgg cacgtttctt tgaagacgat gacggcctgc atattcactc
    15421 cttcttcttc tttgaagatg cggaagatca cgccggtaac tccactgtgg catttaccat
    15481 ccgtgatggt cgtctgttta ctctgcgtga gcgtgaactg cctgcattcc gtctgtatcg
    15541 tatgcgcgcc cgtagccagt caatggtgga cggtaacgcc tacgagctgc tgttggatct
    15601 gttcgaaacc aaaatcgaac agctggcaga tgaaattgaa aatatctaca gcgatctgga
    15661 gcagttgagc cgggtaatta tggaagggca tcagggcgat gagtacgatg aggcgctctc
    15721 cacgctggcg gaactggaag atatcggctg gaaagttcgc ctgtgtctga tggataccca
    15781 gcgtgcgctc aacttcctgg tacgcaaggc gcgtttaccg ggtgggcaac tggagcaggc
    15841 gcgtgaaatc ctgcgagata tcgaatccct gctgccgcat aacgaatccc tgttccagaa
    15901 ggtgaacttc ctgatgcagg cggcaatggg ttttatcaac atcgagcaga accgcatcat
    15961 caaaattttc tcggtggtat ccgtggtatt cctgccgccg acgctcgttg cttccagcta
    16021 tgggatgaac tttgagttta tgccagaact gaagtggagt ttcggctacc caggcgcgat
    16081 tatctttatg atcctcgcgg gcctggcacc gtatctgtac tttaagcgca agaactggct
    16141 gtaatatgag tgccggataa agctattttt ttatccggct tatttctaaa aattgtctta
    16201 tttaaagtca tgcggattga aaactcgctc tattttattt tcttgtgtta attgatactc
    16261 agtaaaatcg ttaataccaa tatgagtgtt acgataaggg tattcattaa taaccattaa
    16321 gtaatttttc acgatgcaat tatcattgct cttaattgtc gggaaacagt agagatcttc
    16381 atacggccct tcgatatatt ttttatagtc agtgaagcca ttaccgatat caatgacata
    16441 ccaggtatgc caactacctt ctggtagggg atgttgattt ttaatttcat tctgatgctt
    16501 cagataccaa tttattttat cgctgtcagt ccatggcaga tgatcaacga ccagaaagac
    16561 tgaattgcca gcatcagagt atgtatacaa aatctgtaca ggacgcaatg tccataaatt
    16621 atagatgcag catag
//
LOCUS       DAZFVO020000113        16497 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFVO020000113 DAZFVO020000000
VERSION     DAZFVO020000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.114"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..93)
                     /locus_tag="ACHKNB_RS17160"
                     /old_locus_tag="pgaptmp_003432"
     CDS             complement(<1..93)
                     /locus_tag="ACHKNB_RS17160"
                     /old_locus_tag="pgaptmp_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196885.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMI"
     gene            complement(221..2182)
                     /gene="topB"
                     /locus_tag="ACHKNB_RS17165"
                     /old_locus_tag="pgaptmp_003433"
     CDS             complement(221..2182)
                     /gene="topB"
                     /locus_tag="ACHKNB_RS17165"
                     /old_locus_tag="pgaptmp_003433"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HGU4196886.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(2187..3230)
                     /gene="selD"
                     /locus_tag="ACHKNB_RS17170"
                     /old_locus_tag="pgaptmp_003434"
     CDS             complement(2187..3230)
                     /gene="selD"
                     /locus_tag="ACHKNB_RS17170"
                     /old_locus_tag="pgaptmp_003434"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HGU4196887.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3347..3898)
                     /gene="ydjA"
                     /locus_tag="ACHKNB_RS17175"
                     /old_locus_tag="pgaptmp_003435"
     CDS             complement(3347..3898)
                     /gene="ydjA"
                     /locus_tag="ACHKNB_RS17175"
                     /old_locus_tag="pgaptmp_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HGU4196888.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4059..5915
                     /gene="sppA"
                     /locus_tag="ACHKNB_RS17180"
                     /old_locus_tag="pgaptmp_003436"
     CDS             4059..5915
                     /gene="sppA"
                     /locus_tag="ACHKNB_RS17180"
                     /old_locus_tag="pgaptmp_003436"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HGU4196889.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6082..7098
                     /gene="ansA"
                     /locus_tag="ACHKNB_RS17185"
                     /old_locus_tag="pgaptmp_003437"
     CDS             6082..7098
                     /gene="ansA"
                     /locus_tag="ACHKNB_RS17185"
                     /old_locus_tag="pgaptmp_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGU4196890.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7109..7750
                     /gene="pncA"
                     /locus_tag="ACHKNB_RS17190"
                     /old_locus_tag="pgaptmp_003438"
     CDS             7109..7750
                     /gene="pncA"
                     /locus_tag="ACHKNB_RS17190"
                     /old_locus_tag="pgaptmp_003438"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HGU4196891.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7911..8183)
                     /gene="yeaC"
                     /locus_tag="ACHKNB_RS17195"
                     /old_locus_tag="pgaptmp_003439"
     CDS             complement(7911..8183)
                     /gene="yeaC"
                     /locus_tag="ACHKNB_RS17195"
                     /old_locus_tag="pgaptmp_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HGU4196892.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8225..8638)
                     /gene="msrB"
                     /locus_tag="ACHKNB_RS17200"
                     /old_locus_tag="pgaptmp_003440"
     CDS             complement(8225..8638)
                     /gene="msrB"
                     /locus_tag="ACHKNB_RS17200"
                     /old_locus_tag="pgaptmp_003440"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGU4196893.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8980..9975
                     /gene="gapA"
                     /locus_tag="ACHKNB_RS17205"
                     /old_locus_tag="pgaptmp_003441"
     CDS             8980..9975
                     /gene="gapA"
                     /locus_tag="ACHKNB_RS17205"
                     /old_locus_tag="pgaptmp_003441"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGU4196894.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10059..10943
                     /gene="yeaD"
                     /locus_tag="ACHKNB_RS17210"
                     /old_locus_tag="pgaptmp_003442"
     CDS             10059..10943
                     /gene="yeaD"
                     /locus_tag="ACHKNB_RS17210"
                     /old_locus_tag="pgaptmp_003442"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HGU4196895.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVTLTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10994..11848)
                     /gene="yeaE"
                     /locus_tag="ACHKNB_RS17215"
                     /old_locus_tag="pgaptmp_003443"
     CDS             complement(10994..11848)
                     /gene="yeaE"
                     /locus_tag="ACHKNB_RS17215"
                     /old_locus_tag="pgaptmp_003443"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HGU4196896.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(11938..12684)
                     /gene="mipA"
                     /locus_tag="ACHKNB_RS17220"
                     /old_locus_tag="pgaptmp_003444"
     CDS             complement(11938..12684)
                     /gene="mipA"
                     /locus_tag="ACHKNB_RS17220"
                     /old_locus_tag="pgaptmp_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HGU4196897.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLCDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13120..15054
                     /gene="yeaG"
                     /locus_tag="ACHKNB_RS17225"
                     /old_locus_tag="pgaptmp_003445"
     CDS             13120..15054
                     /gene="yeaG"
                     /locus_tag="ACHKNB_RS17225"
                     /old_locus_tag="pgaptmp_003445"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HGU4196898.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15167..16450
                     /gene="yeaH"
                     /locus_tag="ACHKNB_RS17230"
                     /old_locus_tag="pgaptmp_003446"
     CDS             15167..16450
                     /gene="yeaH"
                     /locus_tag="ACHKNB_RS17230"
                     /old_locus_tag="pgaptmp_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HGU4196899.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4060 a         4268 c         4067 g         4102 t
ORIGIN      
        1 aatcatccgt tcaaaattac tattaaatat ttcccagcca ttaaaagaat acttctcgct
       61 cccaggatgg ttttgtaata aaactttttt catcacattc cctgttatta catactttag
      121 aggcgataat tttatccatg caaaaaaaat atccaacgaa ttttcttgat ccgctggata
      181 tttcagaata tgactcgaat agcacgaaag attcgctcgc ttacgctatc gccccgcttc
      241 cggcttcatc tgctggcgga ctttttttcg cactacgttt acgcggtgca gcctttttct
      301 tatcagcact gccaccactg cccggagcca caatgccgcg aaactgccgc accggagtac
      361 gtttggcttg atcaataagc tgatatagcg tccccaccag cggctgcata aagtcctgat
      421 agcgacactg cttttcgctg atttgcgtca gcaccgattc ccagtgcgcg gtcatgtccg
      481 gtcgcgtagc catttctggc agcgaatgga ataacgcttt tccggcgtcg gtggagtgga
      541 tatagcgccc ttttttggtg aggaaaccac gcttgaacaa cagttcaata atcccggcac
      601 gcgttgcttc tgtccccaga ccatcggtcg cacgaaggat ctttttcaga tctttgtcct
      661 gcacaaagcg cgcaatcccg gtcatcgccg aaagcagtgt tgcatcggta aaatggcgcg
      721 gcggctgggt ttgccgctct accacttcac ctttttcaca cagcaactca tcgcctttcg
      781 ccaccacagg caatggcgtg ccgtcgtttt cttcatcgcg ctctttgctg cctaacagcg
      841 cgcgccaacc tgcttcagca agaaaacgcg ctttagcgac aaatttgcct ttggcaatgt
      901 ccagttcgat aacacacttg cggaacaccg catccgggca gaactgcatc agatactgac
      961 gggcaatcag gttatagacc ttcgcttcgt tctccgtcag gttgatcgca gaactccttg
     1021 cggtcggaat gatggcgtgg tgcgcatcga cctttttgtc atcccaacag cggttgcgta
     1081 tatctggatc taccactgac tgcggcaaca gatccggtgc atgaacgctg atggcattca
     1141 tcaccgcgtg gcgtccggca aaatgttctt ctggcaaata gcgacaatcg gaacgcggat
     1201 aagtgattag cttgtgcgtt tcgtacagct tctggcagat atcaagcacg ttctgcgcgc
     1261 tcagaccgaa gcgttttgcc gcttcaatct gcaacgccga aagcgaaaac ggcagcggcg
     1321 cggattctga ttcccgttta tcgttatagc tggtgacaat agccggttga ccactaatgc
     1381 ggttaaccac atgctccgcc agtggacgat gtaacaagcg cccttcttca tcctggtacg
     1441 gttcacacgc ttcgctcggt tgccagatag cggtaaaccg ctcatcggca ggtgtcacga
     1501 tatgcgcttt gacttcaaag aagtctttcg ccacgaagtt ttcaatctct tcatcgcggc
     1561 gcaccaccag cccgagcacg ggtgtctgca cgcgtcccac ggaaagtacg ccctgataac
     1621 cggcattgcg accgagaatg gtatacgcac gggtcatatt gatgccgtac agccagtcgg
     1681 cacgcgctcg cgccagcgca gaaacgcaca gcggtacaaa ctcactgttg gaacgaagac
     1741 ggtcgatcgc ccgctcaacc gcctgcgggt tcaggtcgtt tatcaagcaa cgctgcacct
     1801 gctggcgctt ttccggtgcc agttgtagat agtccagcac ttcatccacc agcagttgcc
     1861 cttcgcgatc cgggtccccg gcgtgaacga tttcgctggc ttcatgcagg aaccgtttga
     1921 tgacgttaag ttgtttggtc acggagggtc ggggctgtaa ttgccacttt tccgggacaa
     1981 tcggcaaatc cgcaagattc cagcgcgcat agcggctgtc gtaggcgtct ggctgcgcct
     2041 gctcaagcag gtgaccgata caccaggtca ccacctgacc attaccgcac tcgataaagc
     2101 catcgccttt acggtgcggt ttgggcagga catcagcaat ggcgcgcgcc agactcggtt
     2161 tttcggcaat aaacaaccgc attgaattaa cggatctcaa ccatggcacg accgccgcgc
     2221 gctggcacca gttcgccaat cgccgtcagt tcaatgccaa actcggcggc tgtagcttta
     2281 acctcatttt ctgcttccgg catgaccgcc agcagcaaac cgccagaagt ttgcggatcg
     2341 cacagcagat cgcgcacttc acgcggcatt tcacccatca gatgaccgta gctggcaaag
     2401 ttacgttcgg tgccgcctgg cactgcgccc aacttaatgt actcttcaac ccccggcaat
     2461 ttcgggatcg cgtcatagtc aacgcgcgcc tgcacacctg cgccctgaca catttcgctc
     2521 aaatggccca gcagaccaaa gcccgtaacg tcggtcatcg cttttacgcc ttcgatgttg
     2581 gcaaaggacg cgcctgcgat attcatccgg cacatcactt ctgtcgccag tccctgatgt
     2641 tctggtttca gcagtgattt tttctcagcc gtggtaagaa cgccgatccc cagcggtttc
     2701 gtcaggaaca gtttgcatcc ggcttgtgcg gtactgtttt tcttcacccg ctcggtcggt
     2761 acgatccccg ttaccgccag accaaaaatc ggctccggcg catcgatgga gtgaccgccc
     2821 gccagcgcaa tacccgcctg acggcatgca tagcgtccac cttcggttac ttcgcgggca
     2881 atttccgggg aaagtttgtt aatcggccag ccgaggatcg caatcgccat aatcggtttg
     2941 ccccccatcg cgaagatatc gctgatggcg ttagtcgccg caatgcggcc aaaatcgaaa
     3001 ggattatcaa cgatcggcat aaagaagtcg gtggtactga taacgctggt gccattgccc
     3061 agatcgtaca ccgccgcatc gtcgcgggtc tcattaccca caagcaaatt cggatcaaca
     3121 aacttcgcct gctcactgtg caggatggtt tccaacactt ttggggaaat tttacagccg
     3181 caaccagctc cgtggctgta ttgggtcaaa cgaatcgagt tctcgctcat ggacatctcc
     3241 tgtcaatgca atccgggtat ggtaaccctc attccgtgaa gtgataagtg agagtgtctg
     3301 aattcctgcg cctttgctca caatccagac agtttcgcga caattatcag aaataagtta
     3361 caaacggcgt cgggtccggg acgttaatcg acgtagatgc tttcaactgc ggcgtgccga
     3421 ggtagagaaa accgacaatt ttatcctgct cacggcaacc gaatgcttca cgcactacgg
     3481 gactttcagt taatgcgcca ctgcgccaga tgccgccaaa tccttgggca acggctgcca
     3541 tttgcatcgc catgaccgcg catccggcag acatttcctg ttcccatcgc gggactttat
     3601 gattttcttc gcatttcgcc accaccgtga tgatgagcgg tgcgcggaac ggcgcattac
     3661 gggctttgtc gatagctttg tcatcactac cggcagcaat cgccccctgt tccagtacgg
     3721 cgctgaaacg ctcgcgccct tccccttcaa tcacaaaaaa atgccacggt tgcatggact
     3781 tatggtccgg cgcacgcata cccgcacgca ggatgttttg cagttgttca cccgttggcg
     3841 cgggttcagc caggcgggag gcgctacggc gattgatcaa tagttcgagt gcatccattt
     3901 gattaactcc tgtcgtgata tttattcaca aaattaacac gagagtggat tttgttacag
     3961 cacagtccgc aattcctgct gacaagtgcc ggttgggtca ttacgataac cacatctatt
     4021 gcgcctgtga caggtgtgac cttaagttgg gagaatacat gcgaaccctt tggcgattta
     4081 ttgccggatt ttttaaatgg acgtggcgtc tgctgaattt cgtccgtgaa atggtactta
     4141 acctgttctt tattttcctc gtactggttg gtgtggggat ctggatgcag gtcagtggtg
     4201 gtgattcgaa agaaacggcc agtcgtggcg cactgctgct ggacatttct ggtgtgatcg
     4261 tcgataaacc cgacagttct cagcggttta gtaaattaag ccgccagctg cttggtgcca
     4321 gttccgatcg tctgcaggaa aactcactgt ttgatatcgt caacaccatt cgccaggcga
     4381 aggacgaccg caatatcacc ggtatcgtga tggatctgaa aaacttcgca ggcggcgacc
     4441 agccgtctat gcagtacatc ggcaaagccc tgaaagagtt tcgtgacagc gggaaaccgg
     4501 tttatgccgt tggcgagaac tacagccagg ggcaatatta tctcgccagt ttcgccaata
     4561 aaatttggct gtcaccgcaa ggcgtggttg atctgcacgg ttttgctacc aacggtctgt
     4621 actacaaatc gttgctggat aagctgaaag tttccaccca tgtgttccgc gtgggtacgt
     4681 ataaatctgc cgttgaaccg tttattcgtg atgatatgtc accggcagcc cgcgaagctg
     4741 acagccgctg gattggtgag ctgtggcaaa actatctgaa tactgttgcc gctaaccggc
     4801 agatccctgc tcagcaggta ttccctggcg cgcaagggtt gcttgagggt ttaaccaaaa
     4861 ccggtggcga taccgcgaaa tatgcactgg aaaacaagct ggtcgatgca ctggcatcga
     4921 gtgccgaaat cgaaaaagct ctgaccaaag agttcggctg gagtaagact gataaaaatt
     4981 atcgcgccat cagttattac gattacgcat tgaaaacgcc ggcagatacc ggtgacagca
     5041 tcggtgtcgt cttcgctaat ggcgcaatta tggatggcga ggaaactcag gggaatgttg
     5101 gcggtgatac cactgcggca caaatccgcg acgctcgcct tgacccgaaa gtgaaagcga
     5161 ttgtcctgcg tgttaatagc ccaggcggca gcgttaccgc gtctgaagtg attcgcgctg
     5221 aactggcagc agcccgggca gcgggtaagc ctgtggttgt atcgatgggc ggcatggcgg
     5281 catctggtgg ttactggatt tccacgccag ctaattacat tgtggctaac cccagcaccc
     5341 tgaccggttc tatcggtatc ttcggcgtga tcaccaccgt agaaaatagt ctggattcga
     5401 ttggtgttca tactgatggt gtctcaactt caccgctggc ggatgtttct atcaccaggg
     5461 cactgccgcc ggaagcgcag cagatgatgc aattaagcat tgagaatggc tataaacgct
     5521 ttatcacgct ggttgctgat gcgcgtcatt cgacgccgga gcaaattgat aaaatcgccc
     5581 agggccacgt ctggaccggt caggatgcaa aagctaacgg gctggtcgat agtctcgggg
     5641 atttcgatga tgcggttgcc aaagcagcag agctggcaaa agtgaaacag tggcatctgg
     5701 aatactacgt tgatgaaccg accttcttcg acaaagtgat ggacaacatg tctggttctg
     5761 tccgggcaat gttgccagat gcgttccagg ccatgttacc tgcaccgctt gcctcggtag
     5821 cctctaccgt taaaagtgaa agcgacaagc tggccgcgtt taacgaccca caaaaccgtt
     5881 atgcgttttg cctgacctgc gccaacgtgc gttaagtctt gtactgagtg gccgacagat
     5941 cgtcggccac attatttttt acctcgacga atcctcttcc cgctgtctcg acccatattt
     6001 ccttatccgc atagtatcag gtgcgctcct cctgcctcac gtatatactt ttgctctttc
     6061 gatatcattc atatcaatat catgcaaaag aaatcaattt acgtcgccta cacgggcggg
     6121 accatcggga tgcagcgttc cgagcagggt tatattccgg tgtcaggtca tctacaacgc
     6181 caactggcgc tgatgccgga attccatcgc ccggagatgc cagatttcac cattcatgaa
     6241 tatacgccgt tgatggattc ttctgatatg acgccggaag actggcagca tattgctgaa
     6301 gatattaaag cgcactatga cgactatgat ggttttgtca ttctacacgg taccgacacg
     6361 atggcgtata ccgcctctgc gctgtcgttc atgctcgaga atctcggtaa accggtcatt
     6421 gtgacagggt cacaaatccc gctggctgag ttacgctctg acggacaaat taatctgctg
     6481 aatgcgttgt acgttgcggc gaattatccg atcaacgaag taacgctctt tttcaataac
     6541 cgattgtatc gcggcaaccg cactaccaaa gcccatgccg atggttttga tgcgtttgcc
     6601 tctccaaacc ttcctccgtt actggaagct ggtatccata ttcgtcgttt gaatacgcca
     6661 cccgccccgc acggtgaagg ggaattaatc gttcatccaa tcacgccaca accaattggc
     6721 gtagtgacga tttatccggg gatttctgct gatgtcgtgc gcaattttct gcgccaaccg
     6781 gtgaaagcat tgattctgcg ctcctatggc gtgggtaatg cgccacaaaa caaagccttc
     6841 ctgcaggaat tacaagaagc cagcgatcgc ggtattgtgg tggttaacct gacacaatgt
     6901 atgtccggta aagtgaacat gggtggttat gccaccggta acgccctcgc ccatgccggc
     6961 gtaattggcg gtgcagatat gactgtagaa gctacgctaa ccaaactgca ttacctgctg
     7021 agccaggaac tggatactga aaccattcgc aaggccatga gccaaaacct gcgcggcgaa
     7081 ctgacgccgg atgattaagg agactgcaat gccccctcgc gccctgttac tggtcgattt
     7141 acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg gaaggtgaca gtacggtgga
     7201 tgtcgctaac cgccttattg actggtgcca gtcgcgcggt gaagcggtta tcgccagtca
     7261 ggactggcac ccggcgaatc acggcagttt tgccagtcag cactgtgtag agccttatat
     7321 gccaggtcaa ctcgacggtt tgccacaaac cttctggcca gatcactgtg tgcagaacag
     7381 tgaaggcgca caattacatc cgttactgaa ccaaaaagcg atcgcagcgg tgttccataa
     7441 aggcgaaaat cctttagttg acagttacag tgcctttttt gataacggcc gtcggcagaa
     7501 aacctctctc gatgactggt tacgcgatca tgaaatcgat gaattgatcg ttatgggcct
     7561 ggctactgac tattgcgtga agtttaccgt gctggacgcg ttacagttag gttataaagt
     7621 aaacgtgatt accgatggtt gtcgtggcgt gaatatccag ccccaggaca gtgcgcacgc
     7681 gtttatggag atgtcagcag ctggggcaac gctatatacg ctggcagact gggaagagac
     7741 gcaggggtaa tattagttga attcatctct ggcctacatt tcctcaatga cattgtaggc
     7801 cagataatac ttgtcagtgt tgcatttaac ctacttgcag ctaaaacacg acgtcctaac
     7861 aagtatattt gccctccctt aataacaggc ttaaatacta gataactcat tcacttaaac
     7921 atagcaatag gctttgctga gataccaaaa tcttctttta actgctgttt gctcttcatg
     7981 accatctgac catttgtgtc aattgtcata tgctgtgctt cggtattatg gcgggcttgc
     8041 cacagcatca ccagttgcag gcaattctct ttttgttcct cggtcaacgc aacgccatca
     8101 ggccatttcc ccagttcaac ggcggtcgac aaacgctggt atacctcagg cgtcatgctg
     8161 ttgataatgt catcaagatt cataattttt ccgctcctgt ggaataagtt gctgaatcgt
     8221 tttttcaacc gttgatttct tcgccgtttt cgccatcagt gaagcgtaaa gaagcagagt
     8281 taacacaata acgttcgcct gttggttgcg gcccgtcggg aaagacatgc cccagatggg
     8341 catcacagtt accgcaacga atttctatgc gctgcattcc atgtgacaag tctttgatat
     8401 aacgaatgga ttcttcactc accggttcgt agaaactggg ccagccacag ccggaatcat
     8461 acttggtttc ggaatgaaac agcggggcat cgcagatcaa acagtgatat acgccgtcac
     8521 gcttgttatg cagtaaacga cccgtaaatg gcggttctgt cccatgattc tgcgtcacgt
     8581 aaaactgcat ctcggacaaa ttttttttca gttcttctgc cgaaggttta ttagccattt
     8641 gctcacatct cactttaatc gtgctcacat tacgtgactg attctaacaa aacattaaca
     8701 ccaactggca aaattttgtc ctaaacttga tctcgacgaa atggctgcac ctaaatcgtg
     8761 atgaaaatca catttttatc gtaattgccc tttaaaattc ggggcgccga ccccatgtgg
     8821 tctcaagccc aaaggaagag tgaggcgagt cagtcgcgta atgcttaggc acaggattga
     8881 tttgtcgcaa tgattgacac gattccgctt gacgctgcgt aaggtttttg taattttaca
     8941 ggcaaccttt tattcactaa caaatagctg gtggaatata tgactatcaa agtaggtatc
     9001 aacggttttg gccgtatcgg tcgcattgtt ttccgtgctg ctcagaaacg ttctgacatc
     9061 gagatcgttg caatcaacga cctgttagac gctgattaca tggcatacat gctgaaatat
     9121 gactccactc acggccgttt cgacggtacc gttgaagtga aagacggtca tctgatcgtt
     9181 aacggtaaaa aaatccgtgt taccgctgaa cgtgatccgg ctaacctgaa atgggacgaa
     9241 gttggtgttg acgttgtcgc tgaagcaact ggtctgttcc tgactgacga aactgctcgt
     9301 aaacacatca ctgctggtgc gaagaaagtg gttatgactg gtccgtctaa agacaacact
     9361 ccgatgttcg ttaaaggcgc taacttcgac aaatatgctg gccaggacat cgtttccaac
     9421 gcttcctgca ccaccaactg cctggctccg ctggctaaag ttatcaacga taacttcggc
     9481 atcatcgaag gtctgatgac caccgttcac gctactaccg ctactcagaa aaccgttgat
     9541 ggcccgtctc acaaagactg gcgcggcggc cgcggcgctt cccagaacat catcccgtcc
     9601 tctaccggtg ctgctaaagc tgtaggtaaa gtactgccag aactgaatgg caaactgact
     9661 ggtatggcgt tccgcgttcc gaccccgaac gtatccgtag ttgacctgac cgttcgtctg
     9721 gaaaaagctg caacttacga gcagatcaaa gctgccgtta aagctgctgc tgaaggcgaa
     9781 atgaaaggcg ttctgggcta caccgaagat gacgtagtat ctaccgattt caacggcgaa
     9841 gtttgcactt ccgtgttcga tgctaaagct ggtatcgctc tgaacgacaa cttcgtgaaa
     9901 ctggtatctt ggtacgacaa cgaaaccggt tactccaaca aagttctgga cctgatcgct
     9961 cacatctcca aataagttga gatgacactg tgatctaaaa agagcgactt cggtcgctct
    10021 tttttttacc tgataaaatg aagttaaagg actgcgtcat gattaagaaa atttttgccc
    10081 ttccggtcat cgaacaaatc tcccctgtcc tctcccgtcg taaactggat gaactggacc
    10141 tcattgtggt cgatcatccc caggtaaaag cctcttttgc attacagggc gcacaccttc
    10201 tctcgtggaa acctgcgggt gaagaagaag ttctgtggtt gagcaacaac acaccgttca
    10261 aaaatggcgt cgctattcgc ggtggcgtac cggtttgctg gccgtggttt ggtccggcgg
    10321 cacaacaagg tctgcctgcg cacggttttg cccgcaacct gccgtggacg ctgaaatcac
    10381 atcgtgaaga tgctgatggc gtaacgctga cttttgaatt gacgcaaagc gaagagacga
    10441 aaaaattctg gccgcacgac tttacgctgt tagcgcattt ccgcgtgggt aaaacctgtg
    10501 aaatcgatct tgaatcacat ggcgaatttg aaaccacctc tgccctgcat acctacttta
    10561 acgtgggtga tatcgctaag gtaagcgtca gtgggctggg cgatcgcttc attgataaag
    10621 taaatgacgc gaaagaagat gtactgaccg acggtattca gaccttccct gaccgtaccg
    10681 atcgcgtgta tctgaatccg caagattgca gcgtgattaa tgatgaagcg ctgaatcgta
    10741 ttatcgccgt aggccaccag catcatctga acgttgtcgg ctggaacccg ggaccggcgc
    10801 tttcagttag catgggcgac atgccggatg atggctacaa aacatttgtt tgtgtagaaa
    10861 cggcttacgc ttcagaaacg caaaaagtga ccaaagagaa acctgcacat ctggcgcaat
    10921 ccattcgcgt tgcgaaacgt taatttgcgt taatgttgtg tgccgggtgc gatgcatccg
    10981 gcacgtatca acatcacacc atatccagcg cagtttttcc ttttggtgcc ggatatgctt
    11041 tatccagcat gattaattcc gctgacgaaa gttcgacctc aagcacagcc gcattctgtt
    11101 ggacatgggc aatcgtggcc gcttttggaa tcgccatcac accctgatgg ctgattaccc
    11161 acgccaacaa tacttgtgcc gcgctgatat tgtgagcatg tgcaatttcg ttgactaccg
    11221 cgttttttaa cagtccattg cgcaaccgcc cggcctgggc taatggactg taagccatta
    11281 ccggcatctg ctgttgctgg caccagggga gtagatcgta ctcaatccct cgtgaaccga
    11341 gatggtaaag cacctgatta gtggcacact gatttccccc cggcagctgc cagagttcct
    11401 gcatatcagc ataatcaagg ttagaaacgc cccagcggcg gatttttccc tgggcgatca
    11461 atttttccat cgctgccaca gtctcttcaa aagcgaaact gccagaccag tgtaataagt
    11521 agagatcgag ataatcagta ttgagacggc gtaaactggc ttcgcatgca tttatcgctt
    11581 tttgcccgcc agcattccac ggatagactt tagagacgag aaagacgtta tcgcgcagat
    11641 cggttaatgc ttccccaacc accttttcgg caccgccatc ggcatacatt tcggcggtat
    11701 caatgagggt taaaccgagc tcaatgcccg cgcgtagtgc agcaacttct gttttgcgct
    11761 gactggcatc ttcgcccata taccatgttc cctgccctat ggctggcagt gagacatcgc
    11821 cactaaattg aatcattttt tgttgcattg tttcctccca ggtattgcac caccgtaatg
    11881 caaaacaggg cgtgacgccc tgtttttatg cacaaaatgc cctggaaaga tgcattatca
    11941 gaatttgtag gtgatcccgg tagaaatcag gccagtccag gatttatcca ccatcgggct
    12001 gtcagtaact tcatcagaca gacgggtata gcgcgcggta ccgtaaacac tccagtcgca
    12061 gaggaagttg tagctggcgc tcagctccag gtaagggctc cagctgtcgt tcgggttata
    12121 gccacgcaga ccgctgcgag cggactcttt gcgcgatacg ccataatagt attcgttctg
    12181 gttttcgctg ttccactgca caccaatacc cggagtcacg gtcaggccac cgttggtgta
    12241 acgatacaac caggccatat cccagacaat gccgttgctg ttatccaggg tatcgccagc
    12301 cagggtggta cgcaggtaac cgtactgggt aaagtgagca taagacagac cagccatcat
    12361 ggtgctctta cggtcatcca ggtgacgcat ttggtgatcg ccactgtctt tcgctttgaa
    12421 gtaaagcggc gaccagtaag cggtaattga aagtttatcc gttgcgtcat tccacaggta
    12481 gtaaccacca cctaagccac ggaaccagaa gttatcgcct tcatagttga ttaccggtac
    12541 tgggtaaaca tcggtatcgt aatctttata tgggtgctca acgacaccta cgcctgcgcc
    12601 cagggaaaat ttaccttcag cgtgcgctac gcctgcagac gttgcgataa gcactccaag
    12661 tgccagaagt ttgagtttgg tcacaattaa tcattcctta aacaaatgtt tagcgggcga
    12721 caaagtttac ccgtcaatac atcgaaaccc aaccttttta cgttttcatt ttttaaagta
    12781 actgtttaat tttcctgacg cggatgacac cgcgcttaca gccaaatgaa tttagcgtta
    12841 ctggctaccc tggtctttac attaattatg caaaatttat ggatgagttg ttgatatgcc
    12901 attgaaatta agaaagccgt gcaggcaagt tttccatttg ccatctacgc ttaattttga
    12961 aggtgtatca ccgggcacgt tgttctcatc gtcgataaaa tggcatgaga gttgctgtgt
    13021 tttagcaaga gacgtcgttc agtttacctc ttccgggagc ctctactatt catatgaacg
    13081 gctcttaacc tgtgctaaaa aacgaaagga cggcatacca tgaatatatt cgatcactat
    13141 cgccagcgat atgaagctgc caaggacgaa gagttcacac tgcaggagtt tcttaccact
    13201 tgtcggcaag atcgcagtgc ttatgccaac gcggctgagc ggctattgat ggctatcggt
    13261 gagcctgtca tggtcgatac agcccaggaa cccagacttt ctcgactctt ttctaaccgg
    13321 gtcattgcac gttatccggc atttgaagag ttttacggca tggaagacgc gattgaacag
    13381 attgtctctt atctgaaaca cgcggctcag gggctggaag agaagaaaca aatcctgtat
    13441 ctgctggggc ctgtgggtgg gggtaaatca tcgcttgctg agcgactgaa atcattaatg
    13501 cagctcgtac cgatttatgt attgagcgcc aacggtgagc gtagcccggt caacgatcat
    13561 ccgttctgtc ttttcaatcc gcaggaagat gctcagattc tggaaaaaga gtatggcatt
    13621 cctcgccgtt atctcggcac catcatgtcg ccatgggcgg caaaacgcct gcatgaattt
    13681 ggtggcgata tcactaagtt ccgggtagtg aaggtctggc cgtcaattct gcaacaaatt
    13741 gctatcgcca aaacggaacc cggtgatgag aacaaccagg acatctccgc gctggttggg
    13801 aaagtcgata ttcgtaaact cgaacactac gcgcagaatg acccggacgc ctacggctat
    13861 tccggtgcgc tgtgccgcgc caaccaaggg atcatggaat ttgtcgagat gtttaaagca
    13921 ccgataaaag tgctgcatcc gctgttaacc gccactcagg aaggtaacta caacgggacg
    13981 gaaggtatct ccgccctgcc gttcaacggg attattctcg cccactcgaa cgaatccgaa
    14041 tgggtcactt tccgtaataa caaaaacaac gaagccttcc tcgaccgtgt ttacatcgtg
    14101 aaggtgccgt attgcttacg catttccgaa gagatcaaaa tctacgagaa attgcttaat
    14161 cacagtgaat tgactcacgc cccctgcgcc cctggcacgc tggaaacgct gtcacgtttt
    14221 tccattcttt cgcgccttaa agagccagaa aactccagca tttattcaaa gatgcgggtt
    14281 tatgatggcg aaagtctgaa agacaccgat cccaaagcca agtcgtatca ggaatatcgt
    14341 gactacgccg gtgtcgatga agggatgaac ggtctgtcga cgcgttttgc gtttaagatc
    14401 ctctcccgcg tgttcaactt cgatcatgta gaagtggcgg caaacccggt ccatctgttc
    14461 tacgtactgg aacagcagat cgagcgcgag cagttcccac aagagcaggc agaacgctat
    14521 ctggagttcc tgaaaggtta tctgatcccg aaatatgccg agtttatcgg caaagagatc
    14581 cagacggcct accttgaatc ctattccgaa tatgggcaaa acattttcga ccgttatgtt
    14641 acctacgcgg atttctggat tcaggatcag gagtatcgcg atccggatac cgggcagctg
    14701 tttgaccgcg agtctcttaa cgccgagctg gagaaaatcg agaaaccggc ggggatcagt
    14761 aatccaaaag atttccgcaa cgagattgtt aacttcgtac tgcgcgccag agcgaataac
    14821 agcggacgca atccgaactg gaccagctat gaaaaactgc gcacggttat cgagaagaaa
    14881 atgttctcca ataccgagga gctgttgccg gttatctcgt ttaacgccaa aacgtcaacc
    14941 gacgagcaga agaaacacga cgactttgtt gaccgtatga tggaaaaagg ctacacccgt
    15001 aaacaggtgc gtttactgtg cgaatggtat ttgcgcgtac gtaaatcgtc ttaacaaccc
    15061 tggcccggta cacatgttac cgggcctaca acgacagcga accgtgggcc tgagaagcgg
    15121 caacacaggc gtagcataca gttggcaaat gtagtacggg gggcatatga cctggtttat
    15181 tgaccggcgt ctgaacggca aaaacaaaag catggtgaat cgccagcgtt ttttacgccg
    15241 ttataaagcg caaattaaac agtcgatctc cgaggccatt aataagcgtt cggtgactga
    15301 cgtcgacagc ggcgaatccg tatccattcc cacggaagat attagcgaac cgatgtttca
    15361 tcaggggcgt ggcggtctgc gccaccgcgt gcatccgggc aatgaccatt tcgtccagaa
    15421 cgaccgaatt gaacgtcccc agggtggcgg cggaggttcc ggcagtggtc agggccaggc
    15481 cagccaggat ggtgaaggtc aggatgaatt tgtctttcag atttcgaaag atgagtatct
    15541 tgatttgctc tttgaagatt tggccttacc gaatctgaaa caaaaccaac aacgccagct
    15601 gaccgaatat aaaacgcatc gggcgggtta taccgctaac ggcgttccgg caaatatcag
    15661 cgttgtgcgc tccttgcaga actcactggc gcgacgcaca gccatgacgg caggcaagcg
    15721 gcgggaactt catgcactgg aagagaattt ggccatcatc agcaacagtg aacctgcgca
    15781 gctgctggaa gaggaacgtc tgcgcaaaga aattgcagaa ttacgtgcca aaattgaacg
    15841 cgtccctttt attgacacct tcgatttacg ttacaagaac tacgagaagc ggcccgatcc
    15901 ctccagccag gcagtgatgt tttgcctgat ggacgtttcc ggttcaatgg atcaatccac
    15961 taaagatatg gctaagcgtt tttatattct gctgtatctg ttcctcagca gaacgtataa
    16021 gaacgtggaa gtcgtataca tccgccatca tacccaggcg aaagaagtcg atgaacatga
    16081 gtttttctac tcgcaggaaa caggcggcac catcgtttcc agcgccctga aactgatgga
    16141 tgaggtagtg aaagagcgtt ataacccggc acagtggaat atttacgctg cacaagcatc
    16201 ggacggcgat aactgggccg atgactctcc gctttgccat gaaatcctgg cgaaaaaatt
    16261 attacctgtt gttcgttatt acagctatat cgaaattacc cgtcgtgcac atcagacatt
    16321 gtggcgagaa tatgagcatc tgcaatctac tttcgacaac tttgcgatgc agcacatccg
    16381 cgaccaggat gatatttatc cggtgttccg tgaactgttt cataaacaaa atgcaacagc
    16441 taaagactaa aactatcagc caggtcatta tcgcctggct gatttttagc ttactgt
//
LOCUS       DAZFVO020000114        16418 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFVO020000114 DAZFVO020000000
VERSION     DAZFVO020000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.115"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..539
                     /gene="ygiL"
                     /locus_tag="ACHKNB_RS17235"
                     /old_locus_tag="pgaptmp_003447"
     CDS             <1..539
                     /gene="ygiL"
                     /locus_tag="ACHKNB_RS17235"
                     /old_locus_tag="pgaptmp_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HGU4196900.1"
                     /translation="KSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDED
                     LTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTD
                     LLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSK
                     DATPGNVYAQADYKIAYE"
     gene            599..3103
                     /locus_tag="ACHKNB_RS17240"
                     /old_locus_tag="pgaptmp_003448"
     CDS             599..3103
                     /locus_tag="ACHKNB_RS17240"
                     /old_locus_tag="pgaptmp_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4196901.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3119..3868
                     /gene="yqiH"
                     /locus_tag="ACHKNB_RS17245"
                     /old_locus_tag="pgaptmp_003449"
     CDS             3119..3868
                     /gene="yqiH"
                     /locus_tag="ACHKNB_RS17245"
                     /old_locus_tag="pgaptmp_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4196902.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNQSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3870..4934
                     /gene="yqiI"
                     /locus_tag="ACHKNB_RS17250"
                     /old_locus_tag="pgaptmp_003450"
     CDS             3870..4934
                     /gene="yqiI"
                     /locus_tag="ACHKNB_RS17250"
                     /old_locus_tag="pgaptmp_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4196903.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNMVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4977..5177)
                     /gene="glgS"
                     /locus_tag="ACHKNB_RS17255"
                     /old_locus_tag="pgaptmp_003451"
     CDS             complement(4977..5177)
                     /gene="glgS"
                     /locus_tag="ACHKNB_RS17255"
                     /old_locus_tag="pgaptmp_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HGU4196904.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5446..6075
                     /gene="yqiJ"
                     /locus_tag="ACHKNB_RS17260"
                     /old_locus_tag="pgaptmp_003452"
     CDS             5446..6075
                     /gene="yqiJ"
                     /locus_tag="ACHKNB_RS17260"
                     /old_locus_tag="pgaptmp_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4196905.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6102..7763
                     /gene="yqiK"
                     /locus_tag="ACHKNB_RS17265"
                     /old_locus_tag="pgaptmp_003453"
     CDS             6102..7763
                     /gene="yqiK"
                     /locus_tag="ACHKNB_RS17265"
                     /old_locus_tag="pgaptmp_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4196906.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     GRRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            8002..8061
                     /locus_tag="ACHKNB_RS17270"
                     /old_locus_tag="pgaptmp_003454"
     CDS             8002..8061
                     /locus_tag="ACHKNB_RS17270"
                     /old_locus_tag="pgaptmp_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HGU4196907.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8378..8437
                     /gene="ibsE"
                     /locus_tag="ACHKNB_RS17275"
                     /old_locus_tag="pgaptmp_003455"
     CDS             8378..8437
                     /gene="ibsE"
                     /locus_tag="ACHKNB_RS17275"
                     /old_locus_tag="pgaptmp_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HGU4196908.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8557..9990)
                     /gene="hldE"
                     /locus_tag="ACHKNB_RS17280"
                     /old_locus_tag="pgaptmp_003456"
     CDS             complement(8557..9990)
                     /gene="hldE"
                     /locus_tag="ACHKNB_RS17280"
                     /old_locus_tag="pgaptmp_003456"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HGU4196909.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10038..12878)
                     /gene="glnE"
                     /locus_tag="ACHKNB_RS17285"
                     /old_locus_tag="pgaptmp_003457"
     CDS             complement(10038..12878)
                     /gene="glnE"
                     /locus_tag="ACHKNB_RS17285"
                     /old_locus_tag="pgaptmp_003457"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HGU4196910.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(12901..14202)
                     /gene="ygiF"
                     /locus_tag="ACHKNB_RS17290"
                     /old_locus_tag="pgaptmp_003458"
     CDS             complement(12901..14202)
                     /gene="ygiF"
                     /locus_tag="ACHKNB_RS17290"
                     /old_locus_tag="pgaptmp_003458"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HGU4196911.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14444..15064
                     /gene="ygiM"
                     /locus_tag="ACHKNB_RS17295"
                     /old_locus_tag="pgaptmp_003459"
     CDS             14444..15064
                     /gene="ygiM"
                     /locus_tag="ACHKNB_RS17295"
                     /old_locus_tag="pgaptmp_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HGU4196912.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            15128..16366
                     /gene="cca"
                     /locus_tag="ACHKNB_RS17300"
                     /old_locus_tag="pgaptmp_003460"
     CDS             15128..16366
                     /gene="cca"
                     /locus_tag="ACHKNB_RS17300"
                     /old_locus_tag="pgaptmp_003460"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HGU4196913.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
BASE COUNT         4328 a         4154 c         3883 g         4053 t
ORIGIN      
        1 aaaagtcact tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg
       61 aaggtcacgg cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac
      121 caggtgacga agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg
      181 accagaaatc tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg
      241 gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc
      301 agactgacct gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg
      361 tacgtattct ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat
      421 tcaacaatac taacagctat caggaactga acttcaaagc tcgtatggaa tccccaagca
      481 aagatgctac cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat
      541 cacattaggc cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat
      601 gtataaaaaa ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt
      661 atccgttctc atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat
      721 cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc
      781 cgtaggtaaa tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga
      841 cctgcaatgg gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac
      901 attattaggc tttactgatg agattattga agtggctcag caaaacctga tcgatggttg
      961 ttaccctatc gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt
     1021 atccatatct gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga
     1081 actttgggat catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta
     1141 tgcaccacat cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa
     1201 tcttggggcc tggcgcctgc gtactgatta ccagtacgat caatcattta acaatggcaa
     1261 aagccaggcg aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat
     1321 taatgcaaaa ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca
     1381 tttttctggc gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta
     1441 cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa
     1501 ccgtattatt tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa
     1561 tacattacag gggcaactgg acgtcaaggt tgaagaagag gacgggcaag tcacgcaatg
     1621 gcaagttgca tctaatagta ttccttatct gacgcgtaaa gggcagattc gctacaccac
     1681 tgctatgggt aaaccgacca gcgttggtgg tgattcctta caacaaccct tcttctggac
     1741 tggtgaattc tcatggggtt ggctgaacaa tgtatccctg tatggtggtt cagttttaac
     1801 aaaccgtgat tatcaatctc tggctgccgg cgttggcttt aaccttaact cattgggttc
     1861 attatctttt gatgtcacac gatctgatgc tcagttgcat aatcaggata aagaaacggg
     1921 ttatagctac cgcgctaact attcaaaacg ttttgaatct accggtagcc agctcacttt
     1981 cgctggttac cgtttctctg ataaaaactt tgtgacaatg aatgaatata tcaatgacac
     2041 taaccattac acgaattatc agaatgaaaa agagagttat attgtcacgt ttaaccagta
     2101 tcttgaatca ttaaggttaa atacatacgt aagtttggct cgtaatactt actgggacgc
     2161 cagcagtaat gtgaattatt cattttcact tagccgcgat tttgatatcg ggccattaaa
     2221 aaacgtctcc acttctctaa catttagccg aataaactgg gaagaagaca accaggatca
     2281 actgtaccta aatatttcga ttccctgggg aaccagtaga acattgagct atggtatgca
     2341 acgaaatcag gataacaaga tttcgcatac tgcttcgtgg tatgactctt ccgatcgaaa
     2401 taattcctgg agcgtttctg cttcaggcga caatgatgaa ttcaaagata tgaaagcgtc
     2461 actacgcgcc agttatcagc ataataccga gaacggtcgt ctctatctct ccgggacatc
     2521 acagcgagac agttattatt ctctgaatgc cagttggaat ggttcattca cggcgactcg
     2581 tcacggagcc gctttccatg actatagtgg tagtgctgac tcgcgtttta tgatcgacgc
     2641 agatggtgca gaagatattc cgttgaacaa taaacgcgcg gtaactaatc ggtatggcat
     2701 cggagttatt ccatcagtca gcagttacat aacaacatca ttaagcgttg atacccgaaa
     2761 tcttccagaa aatgtggata tcgaaaactc ggttatcacc accaccttaa ccgaaggtgc
     2821 tattggctac gccaaacttg atacccgcaa gggctatcaa atcatggggg ttattcgcct
     2881 ggcagatggt agtcacccac cactggggat tagcgtaaaa gataaaacca gccataaaga
     2941 attaggactg gttgctgatg gcggctttgt atacctcaac ggcattcagg atgacagcaa
     3001 acttacttta cgctggggtg ataaatcctg ttttattcaa ccacccaata gcagcaactt
     3061 aaccaccgga acggttattt taccgtgtat tagccaaaat taattcagga tgaaactaat
     3121 gcgctactta aacacaaaga atctcattgc agcaggagta ttactcgcct gcatgagcag
     3181 cattgcctgg ggtgccatca tcccggaccg tacccgtatc ataatgaatg agtcagataa
     3241 aggggaagca ttaaaactta ctaatcaaag caaaaatcta ccctatctgg cgcaaacatg
     3301 gatagaagat acgaaaggca atcaatcacg tgactttatt gtcacggttc cccctatggt
     3361 acgtttaaat cccagtgagc aaattcaaat aagaatgata agtcaggaaa aaattgctca
     3421 actgcctaat gacagagaaa cgctcttcta ttttaacgtg cgagaaatac cgccaaaaac
     3481 agataaaaaa aatgtgatgc aagtcactat gcagcacgca ttgaaactat tctggcgacc
     3541 aaaagctatt gaattagaag atgatggggt aatgtcttac gaaaaagttg aaatcatccg
     3601 tagaaatgat ggttcgatcc gctttaacaa taagatgcct tatcacgtta cgctgggtta
     3661 tatcggaaca aacggaatca caatgttgcc acaaacgcaa agtttaatgg taacccctta
     3721 tagccatgca gatacgcagt ttaaaaatgt tccgtcagct tttcaggttg gttatatcaa
     3781 tgattttggt ggattaagtt tttatgaaat aaactgcccg acggttaata actcttgtaa
     3841 tgtttccgta gccaaacgag ataaataata tgcgctactt attaattgta attatattta
     3901 ttataggttt tagtgcatta cctgtatggg caatggactg ctatgctgaa catgaagggg
     3961 gaaataccgt agtcattggt tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag
     4021 gtgataagat ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat aatgcattac
     4081 ctgccccatc acctggagaa gaatacccat cagcctggac taatataatg atgtttttac
     4141 caggcggtca ggacttttat aatcagaact cctatatttt tggcgtaaca tacaatggag
     4201 ttgattatga ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt gatataaaag
     4261 gagcgggtac ctttaataat cactataaaa accccgctgt ctgtagtgga ggtcctgagc
     4321 ctcaattatc agtaaccttt ccagctcgcg tacaactcta tattaaatta gcaaaaaatg
     4381 ccaatagagt taataaaaac atggtgttac ctgatgaata tatcgctcta gagtttaaag
     4441 gtatgagtgg cgcaggagct atagaagtag acaaaaattt gaccttcagg attcgtggat
     4501 taaataatat tcatgtcctt gactgctttg ttaatgtcgc actggaacca gctgatggcg
     4561 ttgttgactt tggtaaaata aattcccgaa caattaaaaa taccagcgtt agtgagacgt
     4621 ttagtgtagt catgacaaaa gatcctggtg cggcctgtac tgagcaattc aatattttag
     4681 ggagcttttt cactacggat attttgagtg attatagcca tctggatatg ggtaatggac
     4741 tgctattgaa gatatttcat aaagatggaa cagcaacgga atttaaccgc ttctcacaat
     4801 ttgcttcttt ttcatcgtct agtgcacctt cggtcaccgc accattcagg gcagaactta
     4861 gtgcgaatcc ggcagagact gttgttgagg gaccgtttag taaagacgtc atcctcaaaa
     4921 tcacctataa ctagcatcta atacaaacac taaaacgggc catcaggccc gttatatcag
     4981 tgctctaact ccagctctct tgcctgcatc atctgttgac aataccaggc ataacgtgcg
     5041 caaaaccagg ttctatgttc ttcatccatg ttaccagtaa cggcaagaat atcgaccggg
     5101 cgaccttctg cgtgcattct ggcaaaacta cgcgccagga aatcgaaatt atttaaagaa
     5161 ttaagactat gatccataag cacatcctcc ggttatcgca taccttttaa ggccatatgc
     5221 tttttctgat ttcagtattc cccaatcagt gtagtgcctt taaacataac gtgcgtaaat
     5281 atattgtccc cgatcacact ttttagtgaa aaataaaaaa ccatattaaa aaactttctc
     5341 ttccagaact tctctaacat gtgttattat taacaaagat aaatgtaagc aaggaaacta
     5401 tcccaaagta aatattgcgt cactaaatgg acattggagt gacatatgat tttattcgcc
     5461 gactataata ccccttatct tttcgctatt tcctttgtac tgttgatcgg ccttttagaa
     5521 attttagcgc ttatctgcgg tcacatgctc tccggtgctc ttgatgcaca tcttgaccat
     5581 tatgattcca taaccacagg tcatattagc caggcacttc attatctcaa tatcggaaga
     5641 ttgccagctc tcgtcgttct ctgcttgctg gctggttttt ttggtcttat tggcatcctg
     5701 ttgcagcatg cctgcatcat ggtctggcaa tcaccactgt cgaacctgtt cgtggttcct
     5761 gtcagtttac tgtttacgat tattgcggtg cattataccg gtaaaatagt tgcgccctgg
     5821 attccacgcg accatagttc agctattaca gaagaagaat atattggtag catggcatta
     5881 attaccggtc atcaagccac ttcaggcaat ccctgcgaag gaaaactcac cgatcaattt
     5941 ggccaaatac attatttact actggagcct gaagaaggaa aatttttcac taaaggagac
     6001 aaagtgttaa ttatttgccg actttctgca acgcgatatt tggcggaaaa caatccctgg
     6061 ccacaaatat tgtaatttat taatcaaaag gaaatttaat aatggatgat attgttaatt
     6121 ctgtgccctc ctggatgttt accgcgatta ttgccgtatg cattctgttt attattggaa
     6181 ttattttcgc caggctctat cgtcgcgctt cggcagagca agcttttgtt cgtactggtt
     6241 taggtgggca aaaagtggtg atgagcggtg gcgcaatcgt gatgccgatc tttcatgaaa
     6301 taatccccat caatatgaat actctgaagc tggaagtcag ccgctcaacc attgatagcc
     6361 tgattacgaa agatcgtatg cgtgtcgatg tagtagtcgc tttctttgta cgggtaaaac
     6421 cgtctgtaga aggcatcgcc actgcggcgc agacgttggg gcaacgcacg ctatcaccag
     6481 aagacttacg tatgttggtt gaagataaat ttgtcgatgc cctccgtgca acagctgcac
     6541 aaatgaccat gcatgagtta caggataccc gcgagaactt cgtgcaggga gtacaaaata
     6601 ctgtcgcaga agacctgtcg aaaaacggtc ttgagctgga aagcgtttca cttaccaact
     6661 ttaaccagac ctcgaaagaa catttcaatc cgaacaatgc ctttgacgcc gaaggtttaa
     6721 ccaaactgac tcaggagacg gagcgccgtc gtcgcgaacg taacgaagtt gaacaggatg
     6781 tagaagttgc ggtgcgtgag aaaaaccgtg atgcactttc gcgcaagttg gagattgaac
     6841 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
     6901 agaatgcacg tattgcggct tttgaagctg gacgtcgtcg tgaagcagag cagacgcgaa
     6961 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
     7021 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacaacag caggtcaccg
     7081 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
     7141 cagaagcgcg tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa
     7201 ccactcgcca gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc
     7261 aggatgcaga aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg
     7321 cagagatgca ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg
     7381 cggaagcaga agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa
     7441 ccagccttaa attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg
     7501 ttgagccgat gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg
     7561 gcagcgctgc gggtgatgca aacacgggta atgtgggggg cggaaacctg gcggaacaag
     7621 cattatcagc cgctctctct taccgcacac aggcaccgct gattgactcc ttgctcaatg
     7681 aaattggcgt ttcaggcggc tcactggcgg cattgacttc acccttaacc tcaacaactc
     7741 ccgtcgccga aaacgtagaa taaattttca gctatgaacg tccctggcac aaaaagccgg
     7801 ggatttttta tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt
     7861 gcgtgaccgc tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc
     7921 tcatcaacaa tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg
     7981 gccttacaga aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttaatc
     8041 gtaagtttcg cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg
     8101 ttcctttact ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc
     8161 tggcccaaaa agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag
     8221 gacgttgcag cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt
     8281 gcgcggggtc cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc
     8341 ccctcccgag gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact
     8401 ggttgtgttg ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa
     8461 tcctccctta cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg
     8521 cctgctacga agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat
     8581 cttcttgatg atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc
     8641 gccgttggcc cagacttctt tactcccggc aatttcttct ggtttatagt cgccgccctt
     8701 caccagtaaa tctggcagga tcccggcgat caagcgctgc ggcgtgtctt cttcaaacga
     8761 caccacccag tcgaccgctt ccagtgcgcc cagcacaatc atacgctgtt cgagcgggtt
     8821 aacggggcgg gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat
     8881 cagacggtca cccagtttgc gagcatttgc cagataggag acgtgcccgg cgtgcaggat
     8941 gtcaaagaca ccgttggtca ttaccacctt ttcgccgcgt ttacgcgctg ccgctacagc
     9001 aagcttcagt tcctcttcgg tcatcacacc aaagccggta tctgcgcgcc cacgcactgc
     9061 attttccagc tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc
     9121 cgccgcattg gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc
     9181 caggacgcca atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt
     9241 tggcatatgc agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt
     9301 cactaacaga gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc
     9361 ttcttcggtc ttacatttac cgacaacagc ttcaaattcc gagagattcg gcgttaacag
     9421 cgtagcgccg cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc
     9481 cgctttacgc gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc
     9541 agaaagcacc agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg
     9601 cggatcaaca ccttcgaaac cttcttcaaa atccaggcgg atcagctgtt ggttgcggga
     9661 aagtacccgt aatttggtga tagtcggatg cgtcggtaca gaaacgaagt cgcatttgac
     9721 gttgacgtcg gccagagatt tactcagcgc acgcgccgca tcgtcaatgc ccgtcaaccc
     9781 gaccaggcgt gcattagcac cgagagaagc gatattcatc gccacgttag ccgcgccgcc
     9841 cggacgttct tcgatggtat tgactttaac cacgggcacc ggcgcttccg gcgagatacg
     9901 gctggtggga ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc
     9961 acgttcaaac tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg
    10021 cgcgataata ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccaga
    10081 tcacgctctg cggtgaagca atcctccgac acatggcccg gcaattcctg taatgccaga
    10141 tgatgaagtt catcgcgcag cgtcgtgtaa gcacgggtca gcgccatcgc ttcctgctct
    10201 tccataatgt cgttttgcgc cagcagctcc agaatacgca cgttgtctga ccagcgcgtt
    10261 aacttcggtt tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg
    10321 gtaattccgc cttcatcggc tttgatatca aagcgattac gatgtttatt gccgagatga
    10381 gcgcgcattt tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttcacgcggc
    10441 agcgtcataa tctcgcgacg cactgcgtca aagtgcgcgg tgagctgcgg atcgccgtac
    10501 actacacgcg cacgcaccag cgcctgatgc tcccacgtcc aggcctcgtt tttctgataa
    10561 tcagcaaatg cttctgcgga tgtcaccagc attcccgccg ccccggacgg acgcagtcgt
    10621 gcatccactt catacaaaat gccggaagag gttcgcgtac tgaacagatg cataatgcgt
    10681 tgcgccagac gcagataaaa ctgtcgcccg tcgatttccc gctcgccgtc agtcatcgca
    10741 tccattgggc aatcatggag gaagatcagg tcaagatcgg aactgtagcc taactcccag
    10801 ccgcccagct tgccgtagcc gaccaccgca aaaccacggc cttcgcgatc gttgaggtga
    10861 tttggcttac cgtagcgggc caccatttga acccacgcct gctgaacgac ggcatctatc
    10921 atagcctctg ccagccaggt taagtgatcg ctcactttca tcaccggtag cgtaccagcg
    10981 atatccgctg cggcgatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc
    11041 tgttgctctt cgtcatcttc cggcacgcgc agcaaatact ggcgcaactc atcgcggtag
    11101 gcatcggtcg ccgtcggctg gtaaagagtg tttggatcga gcaattcatc cagcaataat
    11161 ggataacgcg ccagctggct ggcaatcatc ggcgatgcgg cacacagaga aatcaaatgt
    11221 ttaagcgccg cacggaattc actgagcaat tctaaatagg tggtgcgggt aacaatcccc
    11281 accagcaagg cggtaatgcg cgacagcgta acggcagcgt cttcacgcgc acagacatca
    11341 cttagcagat gtggcatcag atggtcgagc acctgacgtc ctcgcggccc gatagtacgc
    11401 ttatccagct ctttgcggaa atcagcaatc agcgttagga cctgtttgcg atcatcctcg
    11461 ctaagatgcg ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca
    11521 cgccactgtt ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca
    11581 ttaaacaccc ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag
    11641 tcagcaaagt ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtt
    11701 tgggtttgtt cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatat
    11761 gccactcgca attgttcagc atcgttttca gaaagcagat gcagcgcggc aatggcgctg
    11821 agcgttggca gtaaagagcg cgactgcagc gacggttcgc gcccaccgcg aatgagctgg
    11881 aacacctgaa cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg
    11941 gtcaaaccac gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga
    12001 atcacgctga aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg
    12061 ttagcataga cgccttcgct atcgcccata atccgcgcct tgaccatcgc gtaacgctcc
    12121 cagtcgcgcc cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg
    12181 ccactttcgc caaacggacg cagccgcata tccacgcgat agacgaagcc atcctgcgtt
    12241 ggttgatcca acactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc
    12301 agttcccggc gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata
    12361 tctgaggaga aattcagttc cccaccgccc agcttaccca tgcctaaaat cagcagcggt
    12421 tgcgcttcgc cctgcgcatt gcacggcgtt ccccattcgc ggcagcaagc gtcatacagc
    12481 cagtcacgcg cggcgacaat cagcgtctcc gccagatggc tgagctgctg caatatgctc
    12541 tcttcagtaa ccaaagccag cgtttgcgcc caggcgatgc gcaccataat gcgccgccgg
    12601 aatagccgca gctcgcgcat taacccggct tcgtcactca cattgctgag cgcctcctgc
    12661 aaccatgccg cgtaatgctg ccattcatca gcctgcggcg attggctttc cagttccgtc
    12721 agccactctg gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac
    12781 ttcgcctgtg cgctaagtga tttctcggct aaaggctctg gcagccgctc aacaacggtc
    12841 tgccagtact gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga
    12901 ttaacgtttt ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg
    12961 ttcaatttca atgcgttgcc cggtcgcaat agcgtgacgc agcccctgcc agttctccag
    13021 ccaggcttgc gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc
    13081 acgcgtcaga cgtggcaact ggtcacggta gcgatcgccc aacggctggc agaaaacgct
    13141 tttcagttca gcggcatggc gggaaagatg gatatcggca aagcgtttga aggaatcgct
    13201 cattttactc tgcgctttgg catctaaaaa cggctgccag gctttgctta ccaaccattc
    13261 ggtcaacgcc agctttgcca ttgccgtttc ggtagagtag accgccgtca cggcagaaac
    13321 cgcagaagcg atggtcgctt cgcattgagt cagcaaatca cgtaagtgag tgctcgcttt
    13381 acgcggcaca atgccaccga acagcatcag tgcatggcga acaaggccaa tggctgccag
    13441 cacctgctct ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg
    13501 cgctaacgcc agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac
    13561 atgcaaaatg gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc
    13621 acgcgccgct ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc
    13681 cagtttcagc accgcgcgcg tgtcgccgct aagcagttcc agttccagct cacagatagg
    13741 ttcagcaaat tcccccgctt tcacctcccc ctgatcgaga gcgatttcaa ttcggctgtc
    13801 atcgaccgcc accagccatt tttcgcgata aaaatcggtg ctgaacagcg gttgtacgcg
    13861 ggaagcgaga tcggcgggca attcgccgtt cggccagact tccgtcggta actgcgccag
    13921 gtcgagcgtc ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt
    13981 cactcttcct gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa
    14041 gcccatatcg tgcccacgca gccagttatc cggcgtttcg tagtaaatat tcagtaactg
    14101 cacggggtca tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc
    14161 actgtgatta acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt
    14221 atgtcacata agtgacgatg aacggcgaac ttaatacgat cttttttgtc agtagatagt
    14281 attttgcgcc aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg
    14341 gcaagacctg acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg
    14401 ccactactat cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct
    14461 gatcggatta actttactcg cacttagcgc cactgccgtc tcacacgctg aagaaacgcg
    14521 ctatgtttct gatgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct
    14581 cgtgggcacg gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa
    14641 ttatgcccag gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag
    14701 cactgagcca agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac
    14761 cgataaactc accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa
    14821 agtggcgcag agcgacagcg tgatcaacgg attaaaagaa gaaaatcaga aattgaaaaa
    14881 cgagctgatt gtcgcgcaga aaaaggtcga tgccgccagc gtacagttgg atgacaaaca
    14941 gcgcaccatc atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct
    15001 gctcggtctg gtactgccac acctgatccc aacgcgcaaa cgcaaagatc gctggatgaa
    15061 ctaaatcgcc ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga
    15121 tgatggcgtg aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctacc
    15181 ggtcaaagac agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg
    15241 ctaccagcag gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta
    15301 tgcgctggca cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc
    15361 cgcaccggat gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct
    15421 ggcccaggac gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa
    15481 tcgtctgttg cgccatgttt cccccgcttt tggcgaagat ccgttacgcg ttctgcgcgt
    15541 ggcgcgtttt gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac
    15601 gctgatgcgc gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg
    15661 gaaagagacg gaaagcgccc tcaccacccg caatccacag gtgttcttcc aggtactgcg
    15721 cgattgtggc gcactgcgcg ttttattccc ggaaattgac gcactgtttg gcgttccggc
    15781 ccctgccagg tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat
    15841 ggcggcgatg ctgagtccgc aggtcgatgt ccgtttcgca actttatgcc acgatctcgg
    15901 taaaggactg acgccgccag aactttggcc gcgtcatcat ggtcacggtc cggcgggtgt
    15961 gaaattagtg gaacaactct gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc
    16021 cagactggtg gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac
    16081 tatcgtcaaa ttgtttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact
    16141 ggcgctgacc agcgaggctg acgtgcgcgg cagaaccggt tttgaatcag cggactaccc
    16201 gcaaggccgt tggttgcgcg aagcctggga agtggcgcag tcagtgccga caaaagcggt
    16261 cgttgaagcg ggatttaaag gtgtggagat tcgcgaggag ctgacccgac ggcggattgc
    16321 ggcggtagcc agttggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac
    16381 gttaacacga accatcccct cgccccttcg gggagagg
//
LOCUS       DAZFVO020000115        16366 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFVO020000115 DAZFVO020000000
VERSION     DAZFVO020000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.116"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            16..909
                     /gene="ynfL"
                     /locus_tag="ACHKNB_RS17305"
                     /old_locus_tag="pgaptmp_003461"
     CDS             16..909
                     /gene="ynfL"
                     /locus_tag="ACHKNB_RS17305"
                     /old_locus_tag="pgaptmp_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4196914.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            1044..2264
                     /gene="mlc"
                     /locus_tag="ACHKNB_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /gene_synonym="dgsA"
     CDS             1044..2264
                     /gene="mlc"
                     /locus_tag="ACHKNB_RS17310"
                     /old_locus_tag="pgaptmp_003462"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HGU4196915.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            2389..3084
                     /gene="bioD"
                     /locus_tag="ACHKNB_RS17315"
                     /old_locus_tag="pgaptmp_003463"
     CDS             2389..3084
                     /gene="bioD"
                     /locus_tag="ACHKNB_RS17315"
                     /old_locus_tag="pgaptmp_003463"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HGU4196916.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3037..4329)
                     /gene="clcB"
                     /locus_tag="ACHKNB_RS17320"
                     /old_locus_tag="pgaptmp_003464"
     CDS             complement(3037..4329)
                     /gene="clcB"
                     /locus_tag="ACHKNB_RS17320"
                     /old_locus_tag="pgaptmp_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HGU4196917.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4488..5102)
                     /gene="dmsD"
                     /locus_tag="ACHKNB_RS17325"
                     /old_locus_tag="pgaptmp_003465"
     CDS             complement(4488..5102)
                     /gene="dmsD"
                     /locus_tag="ACHKNB_RS17325"
                     /old_locus_tag="pgaptmp_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HGU4196918.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(5145..5999)
                     /gene="ynfH"
                     /locus_tag="ACHKNB_RS17330"
                     /old_locus_tag="pgaptmp_003466"
     CDS             complement(5145..5999)
                     /gene="ynfH"
                     /locus_tag="ACHKNB_RS17330"
                     /old_locus_tag="pgaptmp_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HGU4196919.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(6001..6618)
                     /gene="ynfG"
                     /locus_tag="ACHKNB_RS17335"
                     /old_locus_tag="pgaptmp_003467"
     CDS             complement(6001..6618)
                     /gene="ynfG"
                     /locus_tag="ACHKNB_RS17335"
                     /old_locus_tag="pgaptmp_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HGU4196920.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(6629..9052)
                     /gene="ynfF"
                     /locus_tag="ACHKNB_RS17340"
                     /old_locus_tag="pgaptmp_003468"
     CDS             complement(6629..9052)
                     /gene="ynfF"
                     /locus_tag="ACHKNB_RS17340"
                     /old_locus_tag="pgaptmp_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HGU4196921.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(9113..11538)
                     /gene="ynfE"
                     /locus_tag="ACHKNB_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /pseudo
     CDS             complement(9113..11538)
                     /gene="ynfE"
                     /locus_tag="ACHKNB_RS17345"
                     /old_locus_tag="pgaptmp_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            complement(11737..12042)
                     /gene="ynfD"
                     /locus_tag="ACHKNB_RS17350"
                     /old_locus_tag="pgaptmp_003470"
     CDS             complement(11737..12042)
                     /gene="ynfD"
                     /locus_tag="ACHKNB_RS17350"
                     /old_locus_tag="pgaptmp_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HGU4196922.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            12150..12860
                     /gene="ynfC"
                     /locus_tag="ACHKNB_RS17355"
                     /old_locus_tag="pgaptmp_003471"
     CDS             12150..12860
                     /gene="ynfC"
                     /locus_tag="ACHKNB_RS17355"
                     /old_locus_tag="pgaptmp_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HGU4196923.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(12863..13423)
                     /gene="speG"
                     /locus_tag="ACHKNB_RS17360"
                     /old_locus_tag="pgaptmp_003472"
     CDS             complement(12863..13423)
                     /gene="speG"
                     /locus_tag="ACHKNB_RS17360"
                     /old_locus_tag="pgaptmp_003472"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HGU4196924.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13458..13799)
                     /gene="ynfB"
                     /locus_tag="ACHKNB_RS17365"
                     /old_locus_tag="pgaptmp_003473"
     CDS             complement(13458..13799)
                     /gene="ynfB"
                     /locus_tag="ACHKNB_RS17365"
                     /old_locus_tag="pgaptmp_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HGU4196925.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            13934..14260
                     /gene="ynfA"
                     /locus_tag="ACHKNB_RS17370"
                     /old_locus_tag="pgaptmp_003474"
     CDS             13934..14260
                     /gene="ynfA"
                     /locus_tag="ACHKNB_RS17370"
                     /old_locus_tag="pgaptmp_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HGU4196926.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            14466..15680
                     /gene="rspA"
                     /locus_tag="ACHKNB_RS17375"
                     /old_locus_tag="pgaptmp_003475"
     CDS             14466..15680
                     /gene="rspA"
                     /locus_tag="ACHKNB_RS17375"
                     /old_locus_tag="pgaptmp_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HGU4196927.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            15692..>16366
                     /locus_tag="ACHKNB_RS17380"
                     /old_locus_tag="pgaptmp_003476"
     CDS             15692..>16366
                     /locus_tag="ACHKNB_RS17380"
                     /old_locus_tag="pgaptmp_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HGU4196928.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEK"
BASE COUNT         4021 a         4005 c         4226 g         4114 t
ORIGIN      
        1 taagatgttt taaatatgaa tattgaactt cgtcatctgc gttactttgt tgctgttgcg
       61 gaagagctgc attttgggcg cgcggctgcc cgcctgaata tttcgcaacc gccgctaagt
      121 cagcagattc aggcactgga gcaacaaatc ggtgcccgac tgctggcacg aactaatcgc
      181 agtgtattgc tgacggcagc aggaaaacag tttcttgcag atagtcggca aatcctgtct
      241 atggtggatg acgctgccgc tcgcgctgaa agactgcatc agggtgaagc gggggagttg
      301 cgtattggtt ttacttcgtc cgctcctttt attcgggcgg tgtctgatac gttatcgctg
      361 tttcgccgtg attatcccga tgtccattta caaacccgcg aaatgaacac tcgtgagcaa
      421 atcgctccgc tcattgaagg aacgctggat atgggattgc tgcgtaacac agcgttaccg
      481 gagacgcttg aacacgcagt catcgtccat gaaccgctta tggcgatgat cccgcacgat
      541 catcccctgg caaataaccc gaatgtaacg ctggctgaac tggcgaaaga acccttcgtc
      601 ttttttgatc cgcacgtcgg gacagggctg tatgacgata ttctcgggct gatgcgacgt
      661 taccatttga cgcccgtcat cactcaggag gtgggcgagg caatgaccat catcggtctg
      721 gtttccgccg gtctgggtgt ttcaattttg cctgcgtcat ttaaacgtgt tcagctcaac
      781 gaaatgcgct gggtgccgat tgcagaagag gatgcggttt ctgaaatgtg gttggtctgg
      841 ccgaaacatc atgaacaaag tccagctgcg cgtaactttc gtattcatct gctgaatgct
      901 ctcaggtgag ggaaatttca gcgaaaaagc ccgaaaaatg tgctgttaat cacatgccta
      961 agtaaaaatt tgacgacacg tattgaagtg cttcaccata gcctacagat tatttcggag
     1021 cgcgaaaata tagggagtat gcggtggttg ctgaaaacca gcctgggcac attgatcaaa
     1081 taaagcagac caacgcgggc gcggtttatc gcctgattga tcagcttggt ccagtctcgc
     1141 gtatcgatct ttcccgtctg gcgcaactgg ctcctgccag tatcactaaa attgtccgtg
     1201 agatgctcga agcacacctg gtgcaagagc tggaaatcaa agaagcgggg aaccgtggcc
     1261 gtccggcggt ggggctggtg gttgaaactg aagcctggca ctatctttct ctgcgcatta
     1321 gtcgcgggga gattttcctt gctctgcgcg atctgagcag caaactggtg gtggaagagt
     1381 cgcaggaact ggcgttaaaa gatgactcgc cattgctgga tcgtatcatt tcccatatcg
     1441 atcagttttt tatccgccac cagaaaaaac ttgagcgtct aacttcgatt gccataaccc
     1501 tgccgggaat tattgatacg gaaaatggca ttgtacatcg catgccgttc tacgaggatg
     1561 taaaagagat gccgctcggc gaggctctgg agcagcatac cggcgttccg gtttatattc
     1621 agcatgatat cagcgcatgg acgatggcag aggccttgtt tggtgcctca cgcggggcgc
     1681 gcgatgtgat tcaggtggtt atcgatcaca acgtgggggc gggcgtcatt accgatggtc
     1741 atctgctaca cgcaggcagc agtagcctcg tggaaatagg ccacacacag gtcgacccgt
     1801 atgggaaacg ttgttattgc gggaatcacg gctgcctcga aaccatcgcc agtgtggaca
     1861 gtatacttga gctggcacag ctgcgtctca atcaatccat gagctcgatg ttacatgggc
     1921 aaccgttaac cgtggactca ttgtgtcagg cggcattgcg cggcgatctg ctggcaaaag
     1981 acatcattac cggggtgggc gcgcatgtgg ggcgcattct tgccatcatg gtgaatttat
     2041 ttaacccaca aaaaatactg attggctcac cgttaagtaa agcggcagat atcctcttcc
     2101 cggtcatctc agacagcatc cgtcagcagg cccttcctgc gtatagtcag cacattagcg
     2161 ttgagagtac tcagttttct aaccagggca cgatggcagg cgctgcgctg gtaaaagacg
     2221 cgatgtataa cggttctttg ttgattcgtc tgttgcaggg ttaacatttt ttaactgttc
     2281 taccaaaatt tgcgctatct caatttgggc caggaaagca taacttagac tttcaaggtt
     2341 aattattttc ctggtttata tttgtgaagc ataacggtgg agttagtgat gctgaagcgt
     2401 ttctttatta ccggtacaga cacttctgta gggaaaacgg tggtttcccg cgcattgcta
     2461 caagcgttag cctcccaggg aaaaacggtt gcgggatata aacccgtagc gaaggggagc
     2521 aaagagacac ccgaagggct gcgtaataaa gatgccctgg tgttgcagag tgtttcaacc
     2581 atcgaactgc cttatgaagc agttaatcct atcgcgttaa gcgaagaaga aagtagcgtg
     2641 gcgcacagtt gcccaatcaa ttacaccctc atttcaaacg gcctggcaaa cctgaccgaa
     2701 aaagtcgatc atgtcgtggt agaagggact ggcggctggc gcagtctgat gaatgatttg
     2761 cgtccactct ctgaatgggt ggtgcaggaa caactgccgg tgttgatggt tgtcggtatt
     2821 caggaaggtt gcattaacca tgcactgcta acagctcagg cgattgccaa cgacgggctg
     2881 ccgctcattg gctgggtggc taaccgaatc aacccaggac tggcgcatta tgcggaaatc
     2941 attgatgtgc tcggtaaaaa acttccggca ccgctcattg gtgaactgcc ttatctgccg
     3001 cgcgccgaac agcgtgaact ggggcaatac atccgcttag ctatgctgcg cagtgtgctg
     3061 gcggtagata gagtcacggt gtaacgtccg cgagattacc gacgcaatta cgcaggcaat
     3121 caataaaccg gggagtagct gatactcccc ggtcatttca catatcatca acgtcgacat
     3181 aatcggcgcg tgcgtggttg ccgccaacag tgtcgccatc ccggtcaatc cgagtaaaag
     3241 cgtaatttct tcgccatcag ggaaccataa tcccaggcta cgaccataca acatgccaat
     3301 ggcaagaccg ataaatagcg tcggtgtaaa gacaccaccg ggtgcgccgg aaccgctact
     3361 cgccagcacg gcacacagtt tacaaaggaa gatcccggca atgatcatta acagtggcgg
     3421 ggcggttaaa aaggattgta cggtgctata gccgttgccc cacactgcag gtgtgaacag
     3481 ggaaagcaga cccacgatca acccgcctag tgccagttgc cagggcggcg caagtttgag
     3541 acccacaaat ccacgatgac aggcgttcat taacgttaac aacagtggtc cgcacagacc
     3601 tgccagcaca cctgtactga taatcagcgc atagtcacga gcctgaaccg tcactgagag
     3661 ttgtacgttg tagagtaacg cgtcgctatg attaatcaga ttgctaacca gcaatgccac
     3721 gacggcggaa ataatcaccg ggccgagaga ggccaacatc atagtgccaa acagcacttc
     3781 ggctataaat aaactgccag caagcggggc acgataggcc gcagccattc ccgccgcggc
     3841 cccacaggcg atccataatt tccactcctg gcgtggcgta aaacgttggg caaaacagga
     3901 ggcggcaagg gcagctaaaa gaatcatcgc accttcgcga ccaattgcac tgccgctggt
     3961 tactaccagc agagaggcaa gcgatttaac caggcttgct gcgtaatcga actgtccatc
     4021 ggtttgcaac gcttccatgt aatcggtcgg cgcatgaggg cgttgttggg taaatttctg
     4081 ccagcccatc agcaacaaac ccgccgccag tccgccgagc gccggagtta gcaaccgtcg
     4141 ccagggggaa aggtttgtcg ctgcattgac cagactgccg gagtcattat tgaggaacaa
     4201 ccactccagt agtagcatcg catgacgaaa cccggcaacg gcaaaggccg cgagaatacc
     4261 gacgactgtt gcgataagca gacggcggaa catagtgcgc agatccggat agatgtgtag
     4321 atgatgcatg agcagaaatg ggcaatgaaa cacgggcgac tattttgcgg caaaagaggc
     4381 cagccgcaaa agcagtgcgt gctttaagag ataaaaaaac gtgacactgt aacctattat
     4441 tgccggattg cgcgtaatcg tcaccatccg gcaatattac ggtgatccta tcgaaacagc
     4501 ggtttaaccg cgacaggaat taacagttgt gattgccatt gcgccagcgt taagcgagcc
     4561 agttcaccca gtgcacggta gaaagggtgt tctgcttttt cgataaaaac atcaagaaaa
     4621 cgtgttgacc acggaaaaag gtgccatgcc agcaattctt cgcattctgt ctggcgacca
     4681 ttctccgcca accacgcagc catcaacagc agtgaaccaa aatgatcttc cggttcgttt
     4741 tgcttcattt caaactgaat gcctttctcg cgcatccact gacgaagtgc caatgttgaa
     4801 tcgccaaaca gcacagattc gcgatccagc cagaccgacc cccacggcgg agacggcagt
     4861 gcccacgggc cgacaaacaa acgctgccag gcctgggcgt gagtctcttc acactgtgtc
     4921 tgaaaagccg tcaccagcgg cgctaatgac gcttctggta gaggccactg agtttcccag
     4981 ccgtcactgg tgagtaccgc aaccagaggt gcggcttccg cactctctgg agcgtaataa
     5041 aacagcgcgc ccaacacgcg cgccgcgaca gaaaaattat cttgctgtga aaaatgggtc
     5101 attcctaaca tcctgaaatg tgcgggttgc cccgcaccaa tttgttaacc tgcaatagcc
     5161 attcctactg tcatgtgcag gccataaaag agcacacgcc cgatcatctc accgccaagt
     5221 atgaggatta gccccagaat aagtccggca acgtgaggtt cacggcgacg aatgagcggg
     5281 caaagccagc atccaagacc tgcgcataac aataccacac gccagacctg taaggaggcg
     5341 taatctggta ccagtgcgct ggcttgttgc acggatgagt gaatagacgc cagagacaaa
     5401 ccttgcagca caatcacccc ggcacaagcg atcagcgcca gaacgctaat gatggcaaat
     5461 ggtgtggtat taaatgtaac gcgggctgcc cgcagaattg cggcagcaag tattggaccg
     5521 ctcaacaata ccgtcaggaa gaaagccagc gtagtgtaac cgttatgcca ggttggcaca
     5581 gtgtcgattt gatacacgca ggtcatcatc cagacgaaaa tgacgccgag cgccatgctg
     5641 acaagtaacc agagtttccc caacgcttgc ggcattttac cgatgaccgc caccagccac
     5701 catagtccgc caacggcaaa aaatatggaa ccggcagcaa tctcgttact cagaccagaa
     5761 gctccgattc gattcagcga gttaaacgcc cgcagtggcg atcccaggtg catgacagag
     5821 gcaataaacc cgatgcccat caacagccag aggaaaaaca tgccacggac aatacgctga
     5881 cggtctgcgt catttttggc ggcgaaccag ccaattccgc taacaatcag cgcgcctact
     5941 acgcattgac ccaatacggt aaagataacc agtggccact catgccatcc atttcccatt
     6001 ttacacttcc tccggattag ccagataacc agtggtatcg ccggtcgggc ggctgttggc
     6061 gttaggtttg ataacgatat tgggttttgt gaaatgcgcg cgcggcagtg gcgcgacggc
     6121 agccagagtg ccgtgcttct ggcgcagctc ttcaattgga ccgaactcaa gtgcacgcag
     6181 cgggcaggat tcgacacata tgggttgttt cccctcggcg acgcgcgaat aacaaccatc
     6241 gcacttcgtc atgtgccctt tttcagcatt gtactgtggc gcgccgtacg gacaagccat
     6301 gtggcagtag cggcagccga tacaaacatc ttcatcaacc accacaaagc catcttcacg
     6361 cttatgcatt gcgccgctgg ggcagacttt tgtacatgcg gggtcatcgc aatggttaca
     6421 ggagatggag agataataag cgaacacgtt ctggtgccag acgccattat cttcctgcca
     6481 gtcgccgcca gcgtattcat aaatacggcg gaaactgact tccgggccaa gatctttaaa
     6541 atctttgcac gccagttcgc aggttttaca gccagtacag cggctggaat cgataaaaaa
     6601 tccatattgt gtggtcatgg gctactcctt aaaccttttc gatctggaca agattgctgt
     6661 gcgacgggtt tccctttgcc agcggtgaag ggcggtgaga ggtcagaata ttgatactgc
     6721 cgccgtgatc gacccggtca ccaaacatat ccgctttaag ccacgcacct tgcccgatgg
     6781 cggtaacgcc aggcagaata cgcggagtca cttttgcggc aatcagcatt tctccattat
     6841 tgttaaatac ccgcacggta tcgccatgac ggataccgcg tgcctgagca tcaatggggt
     6901 tgatccacac ctcttgtggg caggcctgct gtaacacatc aatattgccg tagctggagt
     6961 gggtacgcgc tttgtagtgg aagcccgtta actgcagtgg ataggttttc cgcaggggat
     7021 cgtcccagcc atcaaaacct ggggtatacg caggaagggg atgaataatt tcatcttttt
     7081 tcaattccca ggtatctgca atcttcgcca gtcgttcaga ataaatttcg attttccccg
     7141 aaggtgtttt caacgggttt gcctgtggat cttcacggaa tgcgcggaaa gcgacgtagt
     7201 gttcttccgg gcattttttc ttaaagatcc cggtcgtttt catctcctcg tagtcgggca
     7261 tctcagggtt acgttccttc gttttcgcat ggagatattt gatccattca tgctgactgc
     7321 gaccttcagt aaaggtttga taaacgtctg gtcctaagcg tttggcgact tcactcagca
     7381 tccagtagat gggtttgcgt tcaaattttg ctgaggttgc gggttgggcg aggatcacat
     7441 agcccatatt ccctgcagat tcatgagaga taaggtcttc ttgctctgtt ggcatcaggt
     7501 cgggcaacag gatatcgcaa tacttagccg aggccgtcat gaagtggtca atgccaacaa
     7561 tcatctcgca cttgctgtca tcctgaagca cctcatgggt gtgattgatg tcgccatgtt
     7621 gattgatcaa tgtgttactg gcgtagcacc ataaaaactt gatggggaca tccagttttt
     7681 cttttccacg aacaccatca cgggtcgcgg tcatttccgt accatggtcg atggcatctg
     7741 tccatgtaaa gacggaaatc tgcgttttaa caggattctc aagcatcggg aaccattcta
     7801 cccccagatc ccagctacct tcgcgtacac ctgagttgcc gccgtttatg ccgacgttac
     7861 cggtgagaac ggaaagcatg gcaatagcgc gggacgtttg ctcgccgttg gaatgtcgtt
     7921 gtggccccca accctgacaa atataagcag gttttgctga accgatctct cgtgccaact
     7981 ggataatttt ttctgccggg atgctggtga tttttgctgc ccattccggc gttttagcta
     8041 tgccgtcagg cccttcgccc agaatatagg ctttataatg cgcgttacgt ggtgcgttgg
     8101 cgggcagcgt tttttcatcg taaccaacac aatatttgtc gagaaatggc tgatcgacca
     8161 tgttttcagt aatcagtacc caggcaatcg cacaggccag tgcgccatcg gtgccagggc
     8221 gaatgggcag ccattcatct tcacgcccgg cagcagtgtc gttataacgt ggatcgatga
     8281 cgatcatgcg tgcgtttgaa cgttcgcggg cttgctcgac gtagtaagtg acaccaccgc
     8341 cgctcatccg cgtttctgcc gggttatttc cgaacataac gaccagtttc gtattggcga
     8401 tatcatccgg gctgttgcca tcattggcac cgaacatata actcattgcg gcactgatct
     8461 gtgcggtact gtagctgcca tagcgactga gaaaaccacc gcaagagttc atcagacggt
     8521 acgggacgtt tgagttggtg atgtttccgc catctacgcc tgttccgtac aggacatgta
     8581 cagcctcatt gccgtaatct ttcaggatcc gccgaagatt atcactgatg gtatccaggg
     8641 cttcgtccca acttatccgt tcaaatttac cttcaccgcg cttgccgacg cgcttcatgg
     8701 gatatttcaa cctatcagga tgattcatcc gtcggcggat agagcgcccg cgtaaacacg
     8761 ctcgaacctg atggttaccg tagacgtcgt cacctgtcgt atcagactcc acccagtaca
     8821 cggtgtcatc tttcacatgc aaacgtaaca gacagcggct cccgcagtta acggtgcagg
     8881 aactccagac cgctttctct tctaccggag cctctgccgc ccggaccatt tgggaaaatg
     8941 gcagagtgaa agcactgctt gccagcgcaa gactgccaag tgcggaggtt ttcatcagac
     9001 ttctacggct gatttcagcc ttcatgagcg cctctgtggt atggattttc atcattactc
     9061 acttattgct tttcaaacaa aatgtcatgc cagaatttat gattgtcgtg ggttatattt
     9121 tttcgatctc gaccagatta gtgtgctgcg ggtttccctt cgccagtggt gaagggcgca
     9181 gagtggttag cgtattcaca cagccgccat ggtcgatttt atcgccagac atattggcct
     9241 cgtgccaggc tccctggccc atagcgctaa ctccagggag aatacgtggt gttactttgg
     9301 ctggtagccg aacttcgcca cgatggttaa acacccgcac catatcgccg ttggcaatcc
     9361 cacgtttctg cgcatctata gggttgatcc acacctcctg acggcaggca gccttcagga
     9421 gatcaatatt gccgtaggtc gagtgagtac gggatttgta atggaaacca aacagttgca
     9481 gtgggaaggt tctacgttca ggggagtccc agccttcaaa ggtagaggca taaactggca
     9541 gtgggcttat cacttcatct ttttccagtt cccaggtacg ggcaatttcc gccagcttgc
     9601 tggaataaat ttcaatctta ccggaaggtg ttttaagtgg atttgcctcg gggtcgtcac
     9661 gaaatgcttt gtaggcgaca aaatgaccat tgggatcttt acgcttatag atacccattt
     9721 tttcagttcg tcgtaagacg gtaacgccgg atctttggca agcattttgg cgtacagatg
     9781 ttgtaaccat tgttcctgcg tgcgaccttc agtgaacttt tgatagacgt caggtccaag
     9841 acgtttcgcg acttcactca ggatccagta aatcggtttg cgttcgaatt tttcgctggt
     9901 gacaggctgg aggaaaatga gatatcccat gttaccggcg tagtcgttag gaataatatc
     9961 ttcctgctca acggtcatca ggtctggcag cagaatgtcg gcatattttg ccgatgaggt
    10021 cataaagttt tcgatgacca caatcatttc gcatttcgat tcgtcctgca gaatttcatg
    10081 cgttttgttg atgtcagaat gctgattaac gagggtattt cccgcgtagt tccagatgaa
    10141 cttaatgggc acatccagtt tatctttgcc gcggacgccg tcgcggattg ccgtcatttg
    10201 cggaccatga tcgatagcat ctgtccagct gaagcaggag attgacattt tgaccggatt
    10261 atccagcacc ggcaggcgtt ctatggtaat ggtataggtc gattcacgcg cgccactatt
    10321 tccgccgctg atgccgacat tgcccgtcaa aataggtaac atagcaatag cgcgtgcagt
    10381 cagttcgccg tttgcctggc gttgcggccc ccagccctgg cagatataag cgggttttgc
    10441 tgtgccaatt tcacgcgcca gtttgatgat acggtctacc gggataccgg taatttgcga
    10501 agcccactgc ggcgttttcg ctgtgttatc atcaccttca ccaagaatat aggctttata
    10561 gtgactattt ttgggtgcat ctgcgggtaa ggttttttcg tcatagccga cgcagtattt
    10621 atcgagaaaa ggttgatcaa cgagattttc gttaatcaat acccaggcaa tacccgcaac
    10681 cagcgcggca tcggtgcccg ggcgaatagg gagccattca tcttcacgac cagcagccgt
    10741 atcggtatat cgcggatcga taacaatcat tttggcgttc gatttctcgc gcgctttttc
    10801 aagaagataa gtgatgccac cgccgctcat gcgggtttct gccgggttgt taccaaacat
    10861 cacgaccagc ttgctgtttt caatatccgt ggtgctgttg ccatcattac tgccgtaggt
    10921 gtagggcatg gcacaggaaa tttgcgcggt gctgtaggag ccatactgat tgagtgaacc
    10981 gccgtagcag ttcatcaggc gtttgaccgc cgaggctgat ggcgaagagc gggtcatatt
    11041 gccgccaacg atccccgaag agtactgaat atatacagcc tcattgccat attgttcgac
    11101 ggttttcttc aggctactgg cgatagtatc cagggcttca tcccagctaa tccgttcgaa
    11161 tttgccttcg ccgcgtttgc ccacgcgttt cattgggtaa ttcaagcgat cgggatgatt
    11221 aatacgccga cggatggatc gaccacgcaa acaggcgcgt acttgatgat tgccatactc
    11281 gtcgctgccg gtgttgtcag tttccaccca ggtgacttca ttatctttaa catgtagacg
    11341 aagtgcacag cggctaccac agttgacgga acaggcaccc cagatcactt tttcgctggc
    11401 ctgttgtacc gctgccgctg cactgcgcag ggtgaacggt aaagaaaaac cgcctgcagc
    11461 cagcgccaga gaacctatcg cggttgattt aacgagtgtt ctgcggctga tgcccaccat
    11521 tcggtcattt ttggacataa ctcactccct gttctttatc gttatataaa tgtttatata
    11581 ttgaatattt agcgcgctaa caatagaggg agtctaccca ttttgggtta agaattatta
    11641 atccatatca atagaagggt atgagtaata aggtgggatt atgttgtatg ttcaaatcgc
    11701 cggatgtgtc gtatccggcg ttcagtcgat aatgtattac tgcggttcgg caggcgcgcc
    11761 atcctgggta gactgcgcgg gagcagagac gttaccgctg gtggtgcggg tatagagaat
    11821 tttatgcgta tcattagcgc aatggccgac gacctgggaa tcaggctgat caacctggtc
    11881 attgggtaca atacttaacg tgaagctgct ttcgggtacg ccattattga taatgcgctg
    11941 tgatatatcg ctctgtatgc gctcacagga tcccggcgcg gcgagtaccg cgggtgaggc
    12001 gagggcgagc agaagcgcgg cacagcaggt tgagagtttc atcataagct ccttacgcga
    12061 agataacttc tttaagcata gcatttaacg tgtaaagtac tgtatttgct actatgattg
    12121 agaatcatct ctactctctg gtgactgttg tgaaatacaa attactacca tgcttactcg
    12181 cgatattcct cacaggatgt gaccgcacag aggtaacact ttcatttacc cctgagatgg
    12241 ccagtttctc taatgaattc gattttgatc cgctgcgtgg tccggtaaaa gatttcactc
    12301 agacattaat ggatgagcaa ggtgaagtga cgaaacgtgt ttctgggact ttgtcggaag
    12361 aaggctgttt tgattcactc gaattactgg atctggaaaa taataccgtg gtcgctctgg
    12421 tactggacgc caattattac cgtgatgccg agacgctgga gaagagagta cgtttacagg
    12481 gaaaatgcca gctagcagaa ttaccttctg ccggggtgag ttgggaaacc gatgataatg
    12541 gcttcgtgat taaagccagc agcaaacaaa tgcagatgga atatcgctat gatgatcagg
    12601 gttatccact gggtaaaacc acgaaaagta acgacaaaac attatctgtc agcgccacgc
    12661 catcaacgga cccgatcaaa aaattagatt acacagcggt tactttactg aataatcaac
    12721 gagttggtaa tgtaaaacag agctgtgaat atgacagtca cgccaatccg gtggactgtc
    12781 agctgatcat tgttgatgaa ggagtaaaac ccgccgtcga aagggtttac accatcaaaa
    12841 atacgatcga ttattattaa tgctattgtg cggtcggctt caggagagtc tgacccggtg
    12901 ttttgtgctc tgccagatac tgatgctgga atatacacat gcgaatggca ttacgatatt
    12961 gaccattaat aaagaactcg tgcatcaatt caccttcaac cgtaaagcca agcttgcggt
    13021 aaatgtgaat cgctttttca ttctctttat cgacgatcag atacagctta tagagattga
    13081 gaacggtgaa gccatagtcc attgctaatt tggcggcacg ggttgccaga cctttcccct
    13141 gatactccgg ggagataatt atctgaaatt ctgcccggcg atgtacatgg ttaatttcca
    13201 ccagctccac cagaccggct ttttcgccgt cacattccac cacaaagcgt cgttcgctct
    13261 gatcgtgaat atgcttatca tacagatcag agagttcaac aaaggcttcg tagggttcct
    13321 caaaccagta acgcatcaca ctggcgttat tgtcgagttg atgtacatag cgtaaatctt
    13381 cacgctccag cgggcgtagc ttaacactgt gggcgcttgg cataacgtgt ccttacattc
    13441 cttaaatcaa taacaggtta gggggtaata acgcggccag ttcgacggtc caggcagcgc
    13501 aaagtatttg gctcccagta ggcattgatg ttggcgcttt gctcacattt atcgcggtta
    13561 tcaaaagcgg cgtcggcttt atcccactct ttttcagtgc gtttattcac tttctgacgc
    13621 agattgcgcg tgtcattcca ttgctctttt tccatagcgg cgtgctggcg gctttgtgca
    13681 ctgtcgccag actcaatcac cagtttgtta gtttcggcat gaacagttgt gctcagtgcc
    13741 agtgcgcaag gcagcagaaa agcgagcagg ccgattcgtt tgctgagagt gattttcata
    13801 attcattccc tgtatgaatg attaaaggag attctacacc atccactgcg aacgcaaaac
    13861 gtaccaggag ggtgtttata ttgatgatat tatgtcgccc tataactata catgatgtca
    13921 ataagtgaca aagatgatta aaacaacgtt actatttttt gctactgcgc tgtgtgaaat
    13981 tattggatgc tttctgccct ggttgtggtt aaaacgaaac gccagtatct ggctgttgct
    14041 tccggcgggg atttcactgg cgctgtttgt ctggttgtta acgttgcatc cagcggcgag
    14101 tgggcgtgtt tacgcggctt atggtggcgt ttatgtctgc acggcgttga tgtggctgcg
    14161 cgttgtggat ggcgtgaaac tgactcttta tgactggacg ggtgcgttga ttgcgctttg
    14221 cggcatgttg atcattgttg cgggctgggg gcgcacgtag gaacataaat ccattttatc
    14281 aataagataa gaggaagtgt cagctgacaa aaggtattct atttcatctt ttgtcaacca
    14341 ttcacagcgc taatatacgc ctttttttgt gatcactccg gcttttttcg atctttatac
    14401 ttgtatggta gtagctcagt tgcgtagatt tcatgcatca cgacaagcga tgcaaggaat
    14461 cgaacatgaa gatcgtaaag gctgaagttt ttgttacctg tccggggcgt aatttcgtca
    14521 cattaaaaat caccactgag gacggtatta cgggccttgg ggatgccacc ctcaatggac
    14581 gtgagctttc cgtggcctct tatttgcagg atcacctttg tccgcagctt attggtcgcg
    14641 atgcgcaccg tatcgaagat atctggcagt ttttctataa aggtgcttac tggcgtcgcg
    14701 gtccggttac gatgtcggcc atttcagcgg ttgatatggc gctgtgggat attaaagcca
    14761 aagctgccaa catgccgctt taccagttac tcggcggcgc gtctcgtgaa ggggtgatgg
    14821 tttattgcca taccaccggt cacagtattg atgaagctct ggatgattat gcccgtcatc
    14881 aggagctggg attcaaagcc atccgcgtgc agtgcggaat ccctggtatg aaaaccacct
    14941 acggcatgtc gaaaggtaaa ggtctggctt atgaacccgc aaccaaagga cagtggccgg
    15001 aagagcagtt gtggtcgacg gagaaatacc tcgatttcat gccgaaattg tttgacgcgg
    15061 tacgtaacaa gtttggtttt aatgaacatt tgctgcatga catgcaccat cgcttaacgc
    15121 ctattgaagc ggcgcgcttt ggtaaaagca ttgaagatta tcgcatgttc tggatggaag
    15181 acccgacgcc tgcagaaaac caggaatgct tccgtctcat tcgccaacat accgtcacac
    15241 ccatcgcagt gggtgaagtc ttcaacagca tctgggactg caaacaactg attgaagagc
    15301 aactcatcga ttatatccgc accacgctga cccatgcagg cggaattacc ggtatgcgcc
    15361 ggattgccga ttttgcttcg ctgtatcagg tacgtactgg ctcacacggt ccttccgatt
    15421 tgtcaccagt ctgcatggct gcagcgctgc actttgatct gtgggtcccc aatttcggtg
    15481 tccaggaata catgggttat tccgaacaaa tgctcgaagt cttcccgcac aactggactt
    15541 tcgataacgg ctatatgcat ccgggagaca aaccgggtct tggcatcgaa ttcgatgaaa
    15601 agctggcggc gaaatatccc tatgaacctg cttatctacc agtcgcacgt ctggaagatg
    15661 gcacgctgtg gaactggtaa ggagtaagat aatgaaaagc atattaattg aaaaaccgaa
    15721 tcaactggcg attgtcgaac gtgaaatacc caccccgtca gcgggtgaag tacgagtaaa
    15781 agtgaaactt gccggaattt gtggttcaga tagccatatt tatcgtgggc ataatccttt
    15841 tgcgaaatat ccgcgcgtca ttggacatga attctttggc gtcattgatg cggtgggtga
    15901 cggcgtggaa agcgccagag tcggtgaacg tgttgctgtc gatccggtgg tcagctgtgg
    15961 gcattgctat ccgtgctcta taggtaaacc gaacgtttgt acgacacttg ctgtattagg
    16021 tgtgcacgct gacggtggtt tcagtgaata tgccgtggtt ccggcaaaaa atgcgtggaa
    16081 aattcctgaa gcagtggccg atcaatatgc ggtgatgatc gaacctttta ccattgcggc
    16141 taacgtaacc ggacatggtc aaccgactga aaatgatacc gttctggttt atggtgccgg
    16201 tccaatcggc ctgacgatcg ttcaggtatt aaaaggcgtc tataacgtta aaaatgtgat
    16261 tgttgccgat cgcattgatg aacgactgga aaaagcgaaa gagagcgggg cagacagggc
    16321 gattaataac agccagacac cgcttggcga gattttcgct gaaaaa
//
LOCUS       DAZFVO020000116        16017 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFVO020000116 DAZFVO020000000
VERSION     DAZFVO020000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 16017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.117"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..70)
                     /gene="yojO"
                     /locus_tag="ACHKNB_RS17385"
                     /old_locus_tag="pgaptmp_003477"
     CDS             complement(<1..70)
                     /gene="yojO"
                     /locus_tag="ACHKNB_RS17385"
                     /old_locus_tag="pgaptmp_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HGU4196929.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKR"
     gene            179..673
                     /gene="napF"
                     /locus_tag="ACHKNB_RS17390"
                     /old_locus_tag="pgaptmp_003478"
     CDS             179..673
                     /gene="napF"
                     /locus_tag="ACHKNB_RS17390"
                     /old_locus_tag="pgaptmp_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HGU4196930.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            663..926
                     /gene="napD"
                     /locus_tag="ACHKNB_RS17395"
                     /old_locus_tag="pgaptmp_003479"
     CDS             663..926
                     /gene="napD"
                     /locus_tag="ACHKNB_RS17395"
                     /old_locus_tag="pgaptmp_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HGU4196931.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            923..3409
                     /gene="napA"
                     /locus_tag="ACHKNB_RS17400"
                     /old_locus_tag="pgaptmp_003480"
     CDS             923..3409
                     /gene="napA"
                     /locus_tag="ACHKNB_RS17400"
                     /old_locus_tag="pgaptmp_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HGU4196932.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3416..4111
                     /gene="napG"
                     /locus_tag="ACHKNB_RS17405"
                     /old_locus_tag="pgaptmp_003481"
     CDS             3416..4111
                     /gene="napG"
                     /locus_tag="ACHKNB_RS17405"
                     /old_locus_tag="pgaptmp_003481"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HGU4196933.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4098..4961
                     /gene="napH"
                     /locus_tag="ACHKNB_RS17410"
                     /old_locus_tag="pgaptmp_003482"
     CDS             4098..4961
                     /gene="napH"
                     /locus_tag="ACHKNB_RS17410"
                     /old_locus_tag="pgaptmp_003482"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HGU4196934.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4958..5407
                     /gene="napB"
                     /locus_tag="ACHKNB_RS17415"
                     /old_locus_tag="pgaptmp_003483"
     CDS             4958..5407
                     /gene="napB"
                     /locus_tag="ACHKNB_RS17415"
                     /old_locus_tag="pgaptmp_003483"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HGU4196935.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5417..6019
                     /gene="napC"
                     /locus_tag="ACHKNB_RS17420"
                     /old_locus_tag="pgaptmp_003484"
     CDS             5417..6019
                     /gene="napC"
                     /locus_tag="ACHKNB_RS17420"
                     /old_locus_tag="pgaptmp_003484"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HGU4196936.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6032..6655
                     /gene="ccmA"
                     /locus_tag="ACHKNB_RS17425"
                     /old_locus_tag="pgaptmp_003485"
     CDS             6032..6655
                     /gene="ccmA"
                     /locus_tag="ACHKNB_RS17425"
                     /old_locus_tag="pgaptmp_003485"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HGU4196937.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6652..7314
                     /gene="ccmB"
                     /locus_tag="ACHKNB_RS17430"
                     /old_locus_tag="pgaptmp_003486"
     CDS             6652..7314
                     /gene="ccmB"
                     /locus_tag="ACHKNB_RS17430"
                     /old_locus_tag="pgaptmp_003486"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HGU4196938.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7356..8093
                     /gene="ccmC"
                     /locus_tag="ACHKNB_RS17435"
                     /old_locus_tag="pgaptmp_003487"
     CDS             7356..8093
                     /gene="ccmC"
                     /locus_tag="ACHKNB_RS17435"
                     /old_locus_tag="pgaptmp_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HGU4196939.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8090..8299
                     /gene="ccmD"
                     /locus_tag="ACHKNB_RS17440"
                     /old_locus_tag="pgaptmp_003488"
     CDS             8090..8299
                     /gene="ccmD"
                     /locus_tag="ACHKNB_RS17440"
                     /old_locus_tag="pgaptmp_003488"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HGU4196940.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8296..8775
                     /gene="ccmE"
                     /locus_tag="ACHKNB_RS17445"
                     /old_locus_tag="pgaptmp_003489"
     CDS             8296..8775
                     /gene="ccmE"
                     /locus_tag="ACHKNB_RS17445"
                     /old_locus_tag="pgaptmp_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HGU4196941.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8772..10715
                     /gene="ccmF"
                     /locus_tag="ACHKNB_RS17450"
                     /old_locus_tag="pgaptmp_003490"
     CDS             8772..10715
                     /gene="ccmF"
                     /locus_tag="ACHKNB_RS17450"
                     /old_locus_tag="pgaptmp_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HGU4196942.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSIYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGITFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10712..11269
                     /gene="dsbE"
                     /locus_tag="ACHKNB_RS17455"
                     /old_locus_tag="pgaptmp_003491"
     CDS             10712..11269
                     /gene="dsbE"
                     /locus_tag="ACHKNB_RS17455"
                     /old_locus_tag="pgaptmp_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HGU4196943.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11266..12318
                     /gene="ccmH"
                     /locus_tag="ACHKNB_RS17460"
                     /old_locus_tag="pgaptmp_003492"
     CDS             11266..12318
                     /gene="ccmH"
                     /locus_tag="ACHKNB_RS17460"
                     /old_locus_tag="pgaptmp_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HGU4196944.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12353..13000)
                     /gene="narP"
                     /locus_tag="ACHKNB_RS17465"
                     /old_locus_tag="pgaptmp_003493"
     CDS             complement(12353..13000)
                     /gene="narP"
                     /locus_tag="ACHKNB_RS17465"
                     /old_locus_tag="pgaptmp_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HGU4196945.1"
                     /translation="MPEATPFQMMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13320..15911
                     /gene="yejO"
                     /locus_tag="ACHKNB_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /pseudo
     CDS             13320..15911
                     /gene="yejO"
                     /locus_tag="ACHKNB_RS17470"
                     /old_locus_tag="pgaptmp_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         3547 a         3924 c         4678 g         3868 t
ORIGIN      
        1 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
       61 gggtatgcat ctttgacaca tcctttaata tcttagcggc tataaaaatg gcttattaat
      121 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
      181 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
      241 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgctgcga
      301 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag
      361 cgttaatttc aaaaataacg agtgcagctt ctgttacgac tgcgcgcagg cttgccccga
      421 atcgctattt tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc
      481 gtgcctggcg tatcagtctg ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
      541 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
      601 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
      661 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
      721 cagacatcag cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc
      781 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca
      841 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
      901 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
      961 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
     1021 tggtcagcag gaagccatta aatgggataa agcgccgtgc cgtttctgcg gtactggttg
     1081 cggcgttctg gtcggaacgc agcaggggcg tgtggtggcc tgtcagggcg acccggacgc
     1141 accggttaac cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg
     1201 taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg
     1261 cgaatttacc ccaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac
     1321 cgccctgaaa gaaaaaggac cggaatcgat cggtatgttc ggttctggtc agtggactat
     1381 ctgggaaggt tatgccgcgt ccaagctgtt caaagcgggc ttccgttcga acaacatcga
     1441 cccgaacgca cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat
     1501 ggatgagccg atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg
     1561 cgctaacatg gcggagatgc acccgatcct ctggtcacgc atcactaacc gtcgtctctc
     1621 taaccagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
     1681 ggataacggc atcatcttta cgccgcaatc tgacctggtg atcctgaact acatcgccaa
     1741 ctatatcatt caaaacaatg cgataaatca ggacttcttc agcaagcacg ttaacctgcg
     1801 caaaggggcg acggacatcg gctacggttt acgtccgacc catccgctgg aaaaagcagc
     1861 gaagaatccg ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt
     1921 tgctgagtat acgctggaaa aaaccgctga aatgaccggc gtgccgaaag accagttaga
     1981 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
     2041 cttcaaccag catacccgtg gtgtgtgggc caacaacctg gtctacaacc tgcacctgct
     2101 gaccggcaaa atttcccagc cgggttgcgg tccgttctcc ctgaccgggc agccttctgc
     2161 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
     2221 gactaacgag aaacatcgtg atatctgcga gaagaagtgg aatatcccgg gcggcaccat
     2281 tccggcaaaa atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaagct
     2341 gaatgtttat tggaccatgt gtaccaacaa catgcaggcc gggccgaaca ttaatgaaga
     2401 gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac
     2461 agtcagtgcg ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg
     2521 cgcttacggt aacgccgaac gccgtactca gttctggcgt cagcaagtac aggcaccggg
     2581 cgaagcgaaa tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga
     2641 agtatggccg gaagagctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga
     2701 agtgctgtat gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca
     2761 gctgaatgat gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta
     2821 cgcatggttt ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc
     2881 gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg
     2941 taacgacccg tacgtgaaag cgggcgaagg ctataagttc tacggtaaac cggatggtaa
     3001 agcggtgatc ttcgcgctgc cgttcgaacc ggcggcggaa gcaccggatg aagagtacga
     3061 cctgtggctc tctaccggac gcgtcctgga gcactggcac accggcagta tgactcgccg
     3121 tgtgccggaa ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc
     3181 gaaagcgcgc gatctgcgcc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
     3241 gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc
     3301 gttcttcgac gccgcacagc tggttaacaa actaacgctg gacgcgaccg atccgctctc
     3361 gaaagagacg gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc
     3421 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc
     3481 aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
     3541 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
     3601 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc
     3661 ttctggcctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
     3721 tgaggacatt ccgtgcgcca aagtgtgccc aagcggtgcg ctggatcgtg agattgaatc
     3781 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
     3841 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac
     3901 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
     3961 cagcgacgcc tgtaccggtt gcggtaagtg cgaaaaagta tgcgtgctgg aacaaccggc
     4021 aatcaaggtg ttaccgctgt cactggcgaa aggggagtta ggtcaccatt accgcttcgg
     4081 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
     4141 aaaaaggctg gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg
     4201 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca
     4261 gcagtagcct gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc
     4321 ttgccagtgg tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc
     4381 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta
     4441 ccgacttagc gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc
     4501 gccacattcg ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc
     4561 tcatctggga atggatcaac cccgtatctc tgatggggcg cagcctggta atgggcttcg
     4621 gcagcggctc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
     4681 gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg
     4741 tgattaccgt tgcggccagc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
     4801 tttgcccgga accgcatgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
     4861 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
     4921 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
     4981 agcgctgtgt caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc
     5041 taatggcgtc gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg
     5101 gatgccaaaa gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc
     5161 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
     5221 tgtcgaaagt tatcgcacca ctggcgcgcc gcgtatcagt cctactcact ttatggacag
     5281 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
     5341 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg
     5401 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
     5461 gaaatggtgg cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt
     5521 cggcggcatc gtcttctggg gcggctttaa cactgggatg gaaaaagcca ataccgaaga
     5581 gttctgcatt agctgccacg aaatgcgtaa cacggtgtat caggaataca tggattccgt
     5641 gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga
     5701 gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
     5761 tggcgttatt gacacgccgc agaaatttga agctcatcgt ctgacgatgg ctcagaatga
     5821 gtggcggcgc atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta
     5881 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
     5941 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga
     6001 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
     6061 ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga
     6121 gtgggtacaa atcaccggta gcaacggtgc ggggaagaca acgcttctcc gtttgctgac
     6181 tgggttgtct cgccctgacg ctggcgaggt tctctggcaa gggcagccct tgcatcaggt
     6241 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
     6301 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgatgccg cacaatgtct
     6361 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attccggtaa atcaactttc
     6421 tgccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg
     6481 gatcctcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
     6541 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
     6601 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgggggccg catgatgttc
     6661 tggcgcattt tccgtcttga gctgcgcgta gcgtttcgcc atagcgccga aatcgccaac
     6721 ccgctgtggt tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg
     6781 caactgctgg cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg
     6841 ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
     6901 ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta
     6961 accggtctgc cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt
     7021 tatggctggc aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc
     7081 ggtgcaccgg gtgtggcgtt gactgtggga cttaagcgcg gtggtgtgct gctcagcata
     7141 ctggtgttac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
     7201 tccatgcatt tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc
     7261 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
     7321 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
     7381 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
     7441 gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc
     7501 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
     7561 gcatttatgc atcaatggcg gtggcggctt ttattggcct tgtctggcag atgaaaatgg
     7621 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
     7681 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc
     7741 tgacttctga actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct
     7801 tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga
     7861 atctgccgat tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc
     7921 ggatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
     7981 tcctgctgct gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg
     8041 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccctgc
     8101 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
     8161 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg
     8221 cgcaattctg cgtggtgtgg cgcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
     8281 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgccgtg
     8341 ttggcagggc tggcgctgac tatcggtctg gtgctatatg cgctgcgctc gaatatcgat
     8401 ctcttttata cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa
     8461 gtcgggcagc gtctgcgcgt cggcgggatg gtgatgccgg gtagcgtgca gcgcgatccc
     8521 aattcgctaa aagtaacttt caccatttac gacgctgaag gttcagtaga tgtctcttac
     8581 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg
     8641 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg
     8701 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
     8761 gacccagcat catgatgccg gaaattggaa acgggctgct gtgcctggcg ttagggattg
     8821 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg
     8881 cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc
     8941 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata
     9001 cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggctcgctac
     9061 tgctgtgggt gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta
     9121 ttccgctgga tattgtggcc cgcgtactgg cgataatggg gatggtcagc gtcggctttt
     9181 tgctgttcat tctctttacc tctaacccat tctcacgcac gctgccgaac ttcccgattg
     9241 aagggcgcga tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc
     9301 tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt tgccattgct tctttgttgt
     9361 gcgggcgtct ggacagcatt tatgcgcgtt ttactcgtcc gtggacgctg gcggcgtgga
     9421 tcttcttgac gctcggcatt gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg
     9481 gcggctggtg gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga
     9541 ctgcgctgat gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat
     9601 tactgctggc aatcagtgcc ttctcgttgt gtctgctggg gactttcctg gtgcgttccg
     9661 gcgtgctggt atcggtacac gcgtttgcct ctgatccggc acgcggtatg tttatcctcg
     9721 cctttatggt actggtgatt ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag
     9781 ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg
     9841 ttttgctggt cgccgcgatg ctggtggtat tactggggac gctgctgccg ctggtgcaca
     9901 agcaactggg actgggcagt atttcgattg gcgaaccgtt cttcaacacc atgtttacct
     9961 ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg
    10021 atcgcccacg taaaatccgc aatttattga ttatcgcctt catctcaacg ctggtgctgt
    10081 cgctgctgct gccgtggctg ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg
    10141 caatggcctg ctggattgct gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgtg
    10201 gcacgaaaac caccttcagt tattggggaa tggtggcggc tcacctgggg ctggcagtga
    10261 caattgttgg tattaccttt agccagaact atagcgttga gcgtgatgtg cgcatgaagt
    10321 ccggcgatag cgtcgatatt catgaatatc gcttcacctt ccgtgatgtc aaagaggtga
    10381 ctggcccgaa ctggcgtggc ggtgtggcga ctatcggcgt aacgcgcgat ggcaagccgg
    10441 aaacggtgct gtatgcggaa aaacgttatt acaacactgc cgggtcgatg atgaccgaag
    10501 cggcaattga cggcggcatc acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa
    10561 acggcgcgtg ggccgtgcgt ctttactaca aaccatttgt tcgctggatt tgggcgggcg
    10621 ggctgatgat ggcgttgggc ggactgctgt gtctgtttga tcctcgctat cgtaagcgcg
    10681 tgagtccgca aaaaactgcg ccggaggccg tatgaagcgc aaagtattgt taattccgtt
    10741 gattatcttc ctggcgattg ccgcggcgct gctgtggcag ctggcgcgta atgccgaagg
    10801 ggatgatccg accaatctgg aatcggcgct cattggcaag cctgtgccga agtttcgtct
    10861 cgaatcactg gacaatccgg ggcagtttta tcaggcggat gtgctgactc agggcaaacc
    10921 agtgctgctt aacgtctggg cgacctggtg tccgacctgc cgtgcggaac atcaatatct
    10981 gaatcagctt tcagcgcagg gcattcgcgt ggtcggcatg aactataaag acgatcgcca
    11041 gaaggcaatc agctggctga aagagctggg caatccttac gcgctaagcc tgtttgatgg
    11101 cgacggcatg ttagggctgg atctcggtgt ctatggcgcg ccagaaacct tccttattga
    11161 cggcaacggt atcattcgct atcgccatgc gggcgatctc aatcctcgcg tctgggaaga
    11221 agagatcaag ccgctgtggg agaaatacag taaggaggcc gcacaatgag gtttttattg
    11281 ggcgtgctga tgctgatgat ctccggctca gcactggcga ccatcgacgt gttgcagttt
    11341 aaagatgaag cacaggaaca acagttccgt cagctcactg aagaactgcg ctgcccgaaa
    11401 tgccagaaca acagcattgc cgattccaac tcgatgattg ccaccgacct gcgtcagaaa
    11461 gtgtatgaac tgatgcagga aggtaaaagt aaaaaagaga ttgtcgatta tatggtggcg
    11521 cgttacggca acttcgtcac ttacgatccg ccgttaacgc cgctgaccgt gctgctgtgg
    11581 gtgctgccgg tagtggctat tggcattggc ggttgggtca tatacgcccg ctcgcggcgt
    11641 cgggtacgcg tagtgccgga agcgtttcct gaacaaagcg tgcaggaagg taagcgtgcc
    11701 ggatatattg tttatctgcc gggtattgtg gtggcgttaa ttgtggctgg cgtcagctac
    11761 taccagactg gcaattatca gcaggtgaaa atctggcagc aggccacggc acaggctccg
    11821 gcgttgctgg acagggcgct ggatccgaaa gccgatccgc tcaacgaaga agagatgtcg
    11881 cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc cgggagatat agaaggctgg
    11941 attatgttgg gccgcgttgg catggcgctg ggtaacgcta gtatcgccac cgatgcatac
    12001 gctactgcgt atcgcctcga tccgaaaaac agcgatgccg cactgggtta tgctgaagcg
    12061 ttgacacgtt catctgatcc caacgacaac cgcctcggtg gtgaactgct gcgccagttg
    12121 gtgagaagcg accacagcaa tatccgtgtg ttaagcatgt atgcgtttaa tgcctttgag
    12181 cagcagcgat ttggcgaagc cgttgccgcg tgggagatga tgttgaaact cttacctgcc
    12241 aacgatactc gccgtgcggt gattgaacgt agtatcgcgc aggcgatgca acatttgtcg
    12301 ccgcaggaga gtaaataaaa agaggcccga tggtgatgcc atcgggctat ttttattgtg
    12361 ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg tgagcggaca ttgagtttgc
    12421 gcagcagatt gcgaatatgt acttttactg tctgctctga aatattcaac accgaggcaa
    12481 tctgtttatt tgacagcccc tgtgccagct cgtgcagaac atccagctcg cgctccgtca
    12541 gcacgctgaa gggatcttct tccgcgccaa acatttcacg ttcacgtaag tactgattga
    12601 cgcgttcgct aaagactttg ctgcctttcg ctccagcacg aattgcttcc agcaatactt
    12661 ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc gtctatcagt gcaaagacat
    12721 cgctggaggc atctgatacg gtcaggataa taatttgcgc ggtaacgcca tccctgcgca
    12781 aggcattgag ggtatccagg ccactcatac ctttcatatt gagatccagc aagatcacgt
    12841 cgatatccag tctattcgcc agatcgatcg cgctcgcgcc gtcgcccgct tcggcgacca
    12901 cttcaaagcc aggatcaagc tccagtaact gacgaacacc gcgtcgcata agtggatgat
    12961 catccacaat catcatctga aaaggcgttg cttcaggcat agtagtctcc tgaggtttta
    13021 ttagaatcat tatgtttgag cgtagcgttg gtaacgctac caggcgcgtg aatgttaccc
    13081 cattttatgt atggacatgt agtgaaaact acaaccttgt cgttcgcttt ggcatcattt
    13141 tcattataaa gacggaattt tgcatcagct tctcatcaag aaaaacaact aaaaaatcaa
    13201 tcacaatact tgtgcatttc tttaaacagt ttttaaaaac tgactccggg tatggagcta
    13261 tgggtatttt ccgtacccaa tgcttttaac agcaattaat ttcataggat gaaagctcaa
    13321 ttcatcaatc tggttctgtt tctctttgtc gttccgcaat atctgttctg gtggctacag
    13381 cgttatattc acccatagca ttggcatcaa cagttgagta tggtgagaca gttgatggtg
    13441 ttgtcctgga aaaagatatc cagctggttt atgggaccgc caataatacg aaaatcaatc
    13501 ctggcggaga acagcatata aaagaatttg gtgtaagtag taatactgaa attaaaggag
    13561 ggtatcagta cattgaaatg aatggtaccg cagaatactc agtattaaat gacggttatc
    13621 aaattgttca aatgggtggc gcggcaaacc agactaccct caataatggt gtgctacagg
    13681 tttatggcgc agcgaatgat cccacgatta aaggcgggcg cttaatcgtt gaaaaagatg
    13741 ggattaccgt ccttgccgct atcgaaaagg gaggattact ggaggttaaa gaggggggat
    13801 tagcgattgc ggtagatcag aaagcaggcg gtgctattaa agcaagcacg cgggtcatgg
    13861 aggcattcgg aacaaaccgt ctcggtcagt tcgaaatcaa gaatggtatt gctaacaata
    13921 tgctgttgga aaacggcgga agtttgcgag ttgaagaaaa tgacttcgct tataatacta
    13981 ctgtagatag tggcggctta ctggaggtta tggatggcgg gactgtaact ggcgttgata
    14041 aaaaagcagg cggaaaatta attgtctcaa cgaatgcgct ggaagtgagt ggtacaaaca
    14101 gtaaaggcca atttagtata aaagatggtg tgtcaaaaaa ttatgaactg gatgatggtt
    14161 ccgggctcat tgttatggag gacacgcagg ccattgatac tatccttgat gagcatgcca
    14221 ctatgcaatc gctgggaaag gatactggta cgaaagtgca ggcaaatgcg gtatatgatc
    14281 tcggtcgatc agatcagaat ggaagtatca cgtattcctc aaaagccatt tctgaaaata
    14341 tggttatcaa caatggccgc gctaacgtct gggctggcac aatggttaac gtttcagtca
    14401 gagggaatga tggcattctt gaggtcatga agccgcaaat aaattatgca cccgcaatgt
    14461 tggtgggtaa ggtagtggtt tctgagggcg cttcttttag aacgcatggt gccgtggata
    14521 ccagcaaagc ggacgtttcg ctcgaaaata gcgtatggac catcattgcc gatatcacta
    14581 cgacgaacca aaacaccctc ctcaacttag ccaaccttgc gatgtctgac gcaaatgtga
    14641 ttatgatgga tgagccagtg actcgttcat cagtgacggc aagtgcggaa aatttcatta
    14701 cgttgaccac caataccctg tcgggaaacg gcaattttta tatgcgtacc gatatggcta
    14761 atcatcagag cgatcagctc aacgtcaccg gtcaggcaac aggtgatttc aaaatattcg
    14821 tgacggacac cggtgccagc ccggcagcag gagatagcct tacactggta acaacgggcg
    14881 gcggtgatgc tgcatttacg ttgggcaatg ccggaggcgt tgttgatatc ggtacgtatg
    14941 aatatacctt gctggataat ggcaaccata gctggagttt ggcagagaat cgcgcgcaaa
    15001 ttaccccttc aaccactgat gtgctgaata tggcggcagc acaaccgctg gtatttgatg
    15061 cagaactgga caccgtgcgt gagcgtcttg gtagcgtaaa aggcgttaat tacgatacgg
    15121 cgatgtggag ttcggcaatt aacacccgca acaacgtgac cactgatgcg ggcgctggct
    15181 ttgagcaaac attgaccggg ctgacgctcg gtatcgatag ccgtttctcc cgtgaagaaa
    15241 ccagcacaat tcgcggcttg ttctttggtt actctcattc tgatattggt tttgatcgcg
    15301 gcggcaaagg caatgtcgat agctataccc tgggggctta tgccggttgg gagcatcaga
    15361 acggtgccta tgttgatgga gtggtgaaag ttgaccgttt tgccaacacc atccattgca
    15421 agatgagtaa tggggcaaca gcgtttggcg attacaatag taacggcgcg ggtgctcatg
    15481 tcgagagcgg gttccgttgg gttgacggat tgtggagtgt tagaccctat ctggccttta
    15541 ccggctttac cacagatggt caggactaca cgttatcaaa cggcatgcgc gcggatgtgg
    15601 gaaatacccg gatattacgc gctgaagcgg gaacggcggt aagctatcac atggacctgc
    15661 aaaacggtac gacgctggaa ccctgactga aagccgccgt gcgtcaggaa tacgccgatt
    15721 ctaaccaggt gaaagttaat gacgatggca aatttaataa tgatgtggct ggaacccgtg
    15781 gcgtttatca ggctgggata aggtcatcgt ttaccccgac gttaagcggt catttgtcag
    15841 tcagctatgg caatggcgca ggggtagaat cgccgtggaa tactcaggcg ggtgtggcct
    15901 ggacgttctg ataacaaaaa ataaacaggc tgtgatgtgt cacggtctgt ttatcgaatt
    15961 aattgcagat ataaaaaaaa ccaaccgtaa aggttgattt tttcttggga tcacgag
//
LOCUS       DAZFVO020000117        15722 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFVO020000117 DAZFVO020000000
VERSION     DAZFVO020000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.118"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(494..1585)
                     /gene="ychF"
                     /locus_tag="ACHKNB_RS17475"
                     /old_locus_tag="pgaptmp_003495"
     CDS             complement(494..1585)
                     /gene="ychF"
                     /locus_tag="ACHKNB_RS17475"
                     /old_locus_tag="pgaptmp_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGU4196946.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1702..2286)
                     /gene="pth"
                     /locus_tag="ACHKNB_RS17480"
                     /old_locus_tag="pgaptmp_003496"
     CDS             complement(1702..2286)
                     /gene="pth"
                     /locus_tag="ACHKNB_RS17480"
                     /old_locus_tag="pgaptmp_003496"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGU4196947.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2564..2842
                     /gene="ychH"
                     /locus_tag="ACHKNB_RS17485"
                     /old_locus_tag="pgaptmp_003497"
     CDS             2564..2842
                     /gene="ychH"
                     /locus_tag="ACHKNB_RS17485"
                     /old_locus_tag="pgaptmp_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HGU4196948.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2897..4576)
                     /gene="dauA"
                     /locus_tag="ACHKNB_RS17490"
                     /old_locus_tag="pgaptmp_003498"
     CDS             complement(2897..4576)
                     /gene="dauA"
                     /locus_tag="ACHKNB_RS17490"
                     /old_locus_tag="pgaptmp_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HGU4196949.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4701..5648)
                     /gene="prs"
                     /locus_tag="ACHKNB_RS17495"
                     /old_locus_tag="pgaptmp_003499"
     CDS             complement(4701..5648)
                     /gene="prs"
                     /locus_tag="ACHKNB_RS17495"
                     /old_locus_tag="pgaptmp_003499"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGU4196950.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5799..6650)
                     /gene="ispE"
                     /locus_tag="ACHKNB_RS17500"
                     /old_locus_tag="pgaptmp_003500"
     CDS             complement(5799..6650)
                     /gene="ispE"
                     /locus_tag="ACHKNB_RS17500"
                     /old_locus_tag="pgaptmp_003500"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HGU4196951.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6650..7273)
                     /gene="lolB"
                     /locus_tag="ACHKNB_RS17505"
                     /old_locus_tag="pgaptmp_003501"
     CDS             complement(6650..7273)
                     /gene="lolB"
                     /locus_tag="ACHKNB_RS17505"
                     /old_locus_tag="pgaptmp_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HGU4196952.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7487..8743
                     /gene="hemA"
                     /locus_tag="ACHKNB_RS17510"
                     /old_locus_tag="pgaptmp_003502"
     CDS             7487..8743
                     /gene="hemA"
                     /locus_tag="ACHKNB_RS17510"
                     /old_locus_tag="pgaptmp_003502"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HGU4196953.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8785..9867
                     /gene="prfA"
                     /locus_tag="ACHKNB_RS17515"
                     /old_locus_tag="pgaptmp_003503"
     CDS             8785..9867
                     /gene="prfA"
                     /locus_tag="ACHKNB_RS17515"
                     /old_locus_tag="pgaptmp_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGU4196954.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9867..10700
                     /gene="prmC"
                     /locus_tag="ACHKNB_RS17520"
                     /old_locus_tag="pgaptmp_003504"
     CDS             9867..10700
                     /gene="prmC"
                     /locus_tag="ACHKNB_RS17520"
                     /old_locus_tag="pgaptmp_003504"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGU4196955.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10697..11089
                     /gene="sirB2"
                     /locus_tag="ACHKNB_RS17525"
                     /old_locus_tag="pgaptmp_003505"
     CDS             10697..11089
                     /gene="sirB2"
                     /locus_tag="ACHKNB_RS17525"
                     /old_locus_tag="pgaptmp_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HGU4196956.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11093..11902
                     /gene="sirB1"
                     /locus_tag="ACHKNB_RS17530"
                     /old_locus_tag="pgaptmp_003506"
     CDS             11093..11902
                     /gene="sirB1"
                     /locus_tag="ACHKNB_RS17530"
                     /old_locus_tag="pgaptmp_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HGU4196957.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQQEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11938..12792
                     /gene="kdsA"
                     /locus_tag="ACHKNB_RS17535"
                     /old_locus_tag="pgaptmp_003507"
     CDS             11938..12792
                     /gene="kdsA"
                     /locus_tag="ACHKNB_RS17535"
                     /old_locus_tag="pgaptmp_003507"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HGU4196958.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12941..13048)
                     /gene="ldrA"
                     /locus_tag="ACHKNB_RS17540"
                     /old_locus_tag="pgaptmp_003508"
     CDS             complement(12941..13048)
                     /gene="ldrA"
                     /locus_tag="ACHKNB_RS17540"
                     /old_locus_tag="pgaptmp_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196959.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13476..13583)
                     /locus_tag="ACHKNB_RS17545"
                     /old_locus_tag="pgaptmp_003509"
     CDS             complement(13476..13583)
                     /locus_tag="ACHKNB_RS17545"
                     /old_locus_tag="pgaptmp_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196960.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(14012..14119)
                     /locus_tag="ACHKNB_RS17550"
                     /old_locus_tag="pgaptmp_003510"
     CDS             complement(14012..14119)
                     /locus_tag="ACHKNB_RS17550"
                     /old_locus_tag="pgaptmp_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HGU4196961.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(14524..15624)
                     /gene="chaA"
                     /locus_tag="ACHKNB_RS17555"
                     /old_locus_tag="pgaptmp_003511"
     CDS             complement(14524..15624)
                     /gene="chaA"
                     /locus_tag="ACHKNB_RS17555"
                     /old_locus_tag="pgaptmp_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HGU4196962.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         3955 a         3966 c         4001 g         3800 t
ORIGIN      
        1 tatctgcata acaaaaacaa tatgcgccat ttttgcatta ctaataagaa aaagcaaaaa
       61 acatcgatat tcatttttgg tcaatgggtt ttctggccta tcgttgttat tgtgctactg
      121 gttatcattt tcatcttgcc aggatcgcac gtaatcacgt tattcgggga ctttatctgc
      181 gggatattac ccttcaggta agtccttttc tcgccagcat attggagcgt gatcaatatt
      241 gatcagctgt gaagggggtg atgtgtagtg gaccaaaact tacgttatat cactactaat
      301 gagatatccg tcatctgaca ctgatgtctt taattattct cgtggttcac tgtaggcaat
      361 aagcacaaaa gtgtaggatg ttacaagaat gattaggact cggtgaaatg aaaaatccac
      421 gcaattgcgt ggattttata tacttttgcg ctcttcatga gatttagcga aacctcatga
      481 gacaataaat taattagacg ttgaaaagga agttcatcac atcgccatct ttcacgatgt
      541 aatctttacc ttctgcacgc attttgcctg cttctttcgc gccttgttca cctttgtaag
      601 tgatgaaatc ttcaaacgag atggtttgtg cacggataaa gcctttttca aaatcagtat
      661 ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat gcacgcactt
      721 ctttcacccc agcggtgaag taagtttgca ggttcagcag cttataaccg gcacggatca
      781 cacggttcag gcccggctct tccagcccaa gctcctgcat aaactcgtca cgttcttcgt
      841 cgtccagttc ggcaatgtct gcttcaacag cagcacaaac cggaaccaca acagaacctt
      901 ctttcgccgc gatttcacgc acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt
      961 tgacgttggc gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg
     1021 ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc tgcattttcc aactggggca
     1081 ggcatttttc caggaccgcc agctcagctt tcgcgtcttt atcgccacct ttggctttct
     1141 tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt
     1201 tgataacttc aatatcgtca gccgggttaa ctttgccgga aacgtgaatg atgttgtcat
     1261 tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc acggatgttg gtcaggaact
     1321 ggttacccag accttcgcct ttcgatgcgc cttttaccag accggcgata tcgacaaatt
     1381 ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc agccagttga tccaggcgag
     1441 ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt gcagaatgga aagttggccg
     1501 cttcaatacc ggctttggtc agcgcgttga acagggtaga tttcccgacg ttgggcaaac
     1561 cgacgatacc gcatttgaat cccatgattt aaatcacctt aatatcttaa taatcaacct
     1621 gttattgcta acagattgca gaaatggaaa taactttgcc tattatacac ggcactcggc
     1681 aaaaatgccg cagacaacga cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt
     1741 ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc gcttcgtcga tggcttcatc
     1801 aattaacttc tgttcactaa caggcggttt gcctaacaca aaaccgacaa ctttattttt
     1861 atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag ttagggttat tacccaattt
     1921 actgatgatg tctttcagtc cattgtgacc accatggcca ccgcccaatt taaatttggc
     1981 gacgccagga ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg
     2041 gaaaaaactg gccatcgccg caacggcttt gccgctgaga ttcataaatg tagtcgggac
     2101 taacaggcgg acatcttcgc ctccaagagt gactcgcgaa gtataaccaa agaatttagc
     2161 ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc
     2221 attatgtcgc gttgcggcgt attcagcacc ggggttcgcc aggccgacaa tcaatttaat
     2281 cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc gtagtttact ggttgcggcc
     2341 ccgcttgaca aaaaactgcg tatcaaatgc agataacgta ataattgcct gagtggacta
     2401 ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa
     2461 ttgtgatcac gcccgcacat aacccacagg gtgttgtcta tactttacac ataaggaaga
     2521 ggggtattcc ctgttacaac ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc
     2581 ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg tcggcgtggg
     2641 gtattcaatc ctcaaccagt taccacagtt taatatgccc cagtatttcg cacatggtgc
     2701 agtgctaagt attttcgtcg gtgccattct ctggctggcg ggtgcccgtg ttggcgggca
     2761 tgaacaggtg tgcgaccgtt actggtgggt tcgccactat gacaaacgtt gccgccgtag
     2821 cgataatcgc cgtcatagct aacaaataat gcagtttgct gaccagtcag tttgcgctga
     2881 ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg acgattcggg aagaacgcca
     2941 ggcgtcccgg gatcggttga atgccagcgc gagccatagt gcgcagtggc tggaattcca
     3001 cgttgcacac gcgcagttca catccctcgg gcagacgctt cacaaaacgc tggaacgcat
     3061 caagaccacc agcatcaagt accggaaccg catcccactt cagaatcaca atccgtttgc
     3121 cttcaagacg tgactccagg tccgtgaaca agccttcagc agcagcaaaa aacagcgggc
     3181 caataacgcg cagtaccaga acatcgtctg gaacatctac gactaccggt gccaggcgag
     3241 tcatacgtgc gatacgacgc ataaacagca gcgatgccag cacgatcccc acgctgatgg
     3301 caataaccat atcaaacagc acggtcagcg acatgcacag cagcatgaca atgatgtcat
     3361 ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc ttcactcatg ttccacgcca
     3421 ccatcaacag cagggctgcc atagcggaaa gcggcagcca ggagagcagc ggtgccagta
     3481 ccagcagggc aagaataacc agaatagagt ggatcaccgc tgagataggg gaagttgccc
     3541 cggcacggac gttagcggca gaacgcgcga tggcagctgt agcggtaata ccaccaaaga
     3601 acggagcgat aatattcccc agtccctgtc caaccagttc gctgttcgcc ttgtgtttcg
     3661 tcccggtcat accatccagt accacggcgc agagcagaga ttcgattgcg ccgagcattg
     3721 ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc gtgaattctg
     3781 aattaggcag atcccacggc agcaccagtt gcggcagcag ttgcggaata ccgttaccct
     3841 gagaaccatc ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga
     3901 gcaggttaac aatccccatc accgcgcaac cagccagcaa ggccggaagg tgaccgggta
     3961 aacgaatgcc cagacgcggc cagaaaacaa gaatacctag cgtcacaatg ccaatggcag
     4021 catcacccac attaatggtc ggcagcgcca taaataatgc gccgactttt tgtagataat
     4081 gttccgggac atgggccatt tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg
     4141 tgatcccgat acccgaggtg aaacctaagg tgacggaaac cggaatatac tcaatcaggc
     4201 gaccaaagcg tgccagaccc atcagaatca aaaaaatccc cgacagcaag gtcgcaacca
     4261 gcagtcctgc cagtccaaac tgttgcgaca cgggatagag aattaccaca aatgccgcag
     4321 tcggaccgga aacgctaaag cgtgacccac ccgtcagagc aatgacaatc cccgcaacag
     4381 ctgcggtata taaaccgtac tggggtgcca caccactacc aatagccaac gccatcgcca
     4441 gcgggatagc aataatcccg acggttatcc cggcaatcag gtcacgggta aaccgtgcgg
     4501 cagtatattt ttctttccag caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg
     4561 aaaatatttt gttcacaata atgtttcatc cgtgagcgca tcatctgtca actaaatggc
     4621 aggtgaagga ggcataggtc atacaaatgg atattacaga caaaaaaacc cgccgcagcg
     4681 ggtctttgag ccgggttcga ttagtgttcg aacatggcag agatcgattc ttcgttgctg
     4741 atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac gttcggcagt
     4801 gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc aattacagag
     4861 ttacgcaggt tattcgccgc gttgccagag aagatcgggt gagtcgcgta cgcaaataca
     4921 cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc gccagtgtcg
     4981 atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat atgcatcacc
     5041 tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt atcgttcagc
     5101 agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac cacaattggg
     5161 ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa tacgttatca
     5221 accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt cagcacacgg
     5281 tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg tacacgagcg
     5341 gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc ggtgatacga
     5401 cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag gttgtcgtta
     5461 gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt ttcgttgatt
     5521 tgtacactga cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac
     5581 aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa aagcttcata
     5641 tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg
     5701 tgcgggccag gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc aacgtctgga
     5761 acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc tctgtgcaat
     5821 ggggaaagat taacgccttt cgccacaaag ccattgagcc attccggggc ttgctctagc
     5881 acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc
     5941 aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc gcgaaaacgt
     6001 tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt ttctattgac
     6061 ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcaccggagt cggaatactt
     6121 acaccagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt tagtatttca
     6181 ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc
     6241 gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag atgatttaat
     6301 gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc catcggcaaa
     6361 cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca
     6421 gtttttatca acaatcgcgc tgcgcgaacg atcaggttat cttcatgttc cacgccttca
     6481 acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat ggtgtcgccg
     6541 taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg ctgaccggta
     6601 atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat tatttcacta
     6661 tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg
     6721 gcatcgcagg ttgcgttttg gtgtcataac caccataaac aaccttccag tttttgccat
     6781 tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag tcggttgcat
     6841 cacccggtaa acctaaaatc cactggcgca agctgttgag cggaattggc attccggtca
     6901 atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta
     6961 actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat gggttagtga
     7021 gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg tacacttttt
     7081 gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga ttgcgcacgt
     7141 cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct ttgggcgtgg
     7201 taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata agacgaaaat
     7261 cgggcagggg catagtgatg acaagtcctt gagatacgtt gcagttataa cccttaatgc
     7321 tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga atctaacggc
     7381 tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca tcctgtatga
     7441 tgcaagcaga ctaaccctat caacgttggt attatttccc gcagacatga cccttttagc
     7501 actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc
     7561 ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt
     7621 ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc aggataacct
     7681 gcaagaggcg ttaatccgct ggctttgcga ttatcacaat cttaatgaag aagatctgcg
     7741 taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc gtgttgccag
     7801 cggcctggat tcactggttc ttggggagcc gcagatcctc ggtcaggtta aaaaagcgtt
     7861 tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt tccagaaatc
     7921 tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg ctgtgtctgt
     7981 cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta cggtcacagt
     8041 gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc gcgaacataa
     8101 agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga
     8161 agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg aagccgatat
     8221 catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg tggagcgcgc
     8281 attaaaaagc cgtcgcaacc aaccaatgct gttggtggat attgccgttc cgcgcgatgt
     8341 tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg atctgcaaag
     8401 catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt
     8461 cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca gcgaaaccat
     8521 tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca aagcgttagc
     8581 ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat ggaaactgac
     8641 taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg acggggataa
     8701 cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca tcattttctt
     8761 tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact ggaagccctg
     8821 catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag
     8881 gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt
     8941 accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct cgatgatcct
     9001 gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg
     9061 gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg taacgccttc
     9121 ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcagg cgatctgttc
     9181 cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg tagaaatcat gagcgccagc
     9241 gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga tggtgtgtat
     9301 ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc tacggaatcg
     9361 cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact gcctgacgca
     9421 gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc gtcaggggcg
     9481 ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt gccgaccggg
     9541 attgttgttg aatgtcagga tgaacgttca caacataaaa acaaagcgaa ggcgctttca
     9601 gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac gccaacaggc cgaagcgtct
     9661 acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac ttacaatttc
     9721 ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgcct ggatgaagtg
     9781 atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca ggccgaccaa
     9841 ctggcggcgt tgtccgagca ggaataatgg aatatcaaca ctggttacgt gaagcaataa
     9901 gccaacttca ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg gagcatgtta
     9961 ccggcagagg gcgtactttt attctcgcct ttggtgaaac gcagctgact gacgaacaat
    10021 gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa
    10081 ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc ttaattccgc
    10141 gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa caaccttgcc
    10201 gtattcttga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct agcgagcgcc
    10261 cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg gcacaacgta
    10321 atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg tttagcgcgc
    10381 tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac gagcaggacc
    10441 cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt gcggcagaca
    10501 gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg gtatccggcg
    10561 gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa gcatttatcc
    10621 tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag cgcgtaacgc
    10681 tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc ttattagtat
    10741 cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga agcatccaca
    10801 ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt tattgttaag
    10861 cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg cacagtggct
    10921 gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg cactcgatta
    10981 tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt tggtggtgct
    11041 gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag tcatgagatc
    11101 gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga
    11161 agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc gtctcgttag
    11221 tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagcagg aaaaactgat
    11281 cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc gtctttccga
    11341 tgcattatgg ctggaccagg tgttaaagaa tcgacagggc agtgcggtat cattaggtgc
    11401 ggttttatta tgggtcgcga atcgtctcga tttgccgctg ctgccggtga ttttccctac
    11461 gcagctgata ttgcgcattg aatgtccgga tggcgaaatt tggctgatta atccttttaa
    11521 cggtgaatcg ttaagcgaac atatgctgga cgtatggtta aagggaaata tcagcccgtc
    11581 ggcggaactg ttttatgaag accttgatga agctgataac attgaggtaa tccgcaaatt
    11641 gctggataca ctcaaagcct cgttgatgga agaaaatcag atggagctgg cgttacgcac
    11701 cagcgaagct ttattacaat tcaaccctga agatccctat gaaattcgcg atcgcgggtt
    11761 gatttatgcg caactggatt gcgaacacgt tgcgttgaac gatttaagtt atttcgttga
    11821 acagtgtccg gaagacccga tcagcgaaat gatccgtgcg caaataaata acatcgcgca
    11881 taaacatatt gtgctgcatt aattaatcga cattttactc aagattaagg cgatcctatg
    11941 aaacaaaaag tggttagcat tggcgacatc aacgtagcaa atgacctgcc gttcgtactg
    12001 tttggcggta tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg cgagcactac
    12061 gtaactgtga cccagaaact gggtatccct tacgtgttca aagcctcttt tgacaaagcc
    12121 aaccgctcct ccatccactc ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc
    12181 caggagttga agcagacttt tggcgtgaaa attatcaccg acgttcacga accaagtcag
    12241 gcacagcccg ttgctgatgt cgtggatgtg attcagttgc cggcgtttct tgctcgccag
    12301 actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag
    12361 tttgtcagcc cgggacagat gggtaatatc gttgataaat tcaaagaagg cggcaacgaa
    12421 aaagtgattc tttgcgatcg tggtgctaac ttcggctatg acaacctggt tgtcgatatg
    12481 ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc
    12541 cacgcactgc aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg tgctcaggtg
    12601 gctgagctgg cacgagccgg tatggcggta ggtctggcgg ggctgtttat tgaagcgcat
    12661 ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc taaactggaa
    12721 ccgttcctca agcagatgaa agcgattgat gatctggtga aaggtttcga agaactggat
    12781 accagcaagt aatctttttt gcttgaaaaa taaagtctta gcgttctgcg ttaagacttt
    12841 tttcatgggt gccagataca aaaaaggccg caggctgtta cccctgcggc cggtttcggg
    12901 cgcatattgc catcacggca gcctgacgcc cgttttcacc ttacttccgg ttacgccacc
    12961 agccgacaat cgctgcggta ataattcccg ccaggatcgg tgctgccagg tcgtgccaga
    13021 aaatcatggc aaactgcgcg agcgtcatat agccgccttg ttgtaatgac aacattttgc
    13081 ggctattctt gaattgttct ggttcaagat tagcccccgt tctgttgtca ggttttacct
    13141 ctcaacgtgc gggggttttc tctttccagc aaccaatgcc accagggata aagcccccgc
    13201 aacattgcgc ctcaccggat aacgccggct tgatgtggat actacgtctc aattcatctt
    13261 cacttcatcc ctgaaatgtt tgcaatgaag agtgcattcc ggtttttcaa cagctgttac
    13321 agtcgtttca tgagggctct ggatgatgct tccagctcgg gttgccaata tttacttgtg
    13381 gaagagataa agacaaaaat ggccgcaggc tgttacccct gcggccggtt tcgggcgcat
    13441 attgccatca cggcagcctg acgcccgttt tcaccttact tccggttacg ccaccagccg
    13501 acaatcgctg cggtaataat ccccgccagg atcggtgctg ccaggtcgtg ccagaaaatc
    13561 atggcaaact tcgcgagcgt catatagccg ccttgttgta atgacaacat tttgcggcta
    13621 ttcttgaatt gttgatggtt caagattagc ccccgttctg ttgtcaggtt ttacctctca
    13681 acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca
    13741 ttgcgcctca ccggataatt ccggcttggt gtggatacta cgactcaatt catcttcact
    13801 tcatccctga aatgtttgca atgaagagtg cattccggtt tttcaacagc tgttacagtc
    13861 gtttcatgag tactctggat gatgcttcca gctcgggttg ccaatattta cttgtggaag
    13921 agataaagac aaaaatggcc gcaggctgtt acccctgcgg ccggtttcgg gcgcatattg
    13981 ccatcacggc agcctgacgc ccgttttcac cttacttccg gttacgccac cagctgacaa
    14041 tcgctgcggt aataatcccc gccaggatcg gtgctgccag gtcgtgccag aaagtcatgg
    14101 caaactgcgc gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct
    14161 tgaagtgtct ggtttcaaga ttagcccccg ttttgttgtc aggttttacc tctcaacgtg
    14221 cgggggtttt ctctttccag caaccaatgc caccagggat aaagcccccg caacattgcg
    14281 cctcaccgga taattccggc ttggtgtgga tactacgtct caattcatct tcacttcatc
    14341 cctgaaatgt ttgcaataaa gagtacattc cggcttttca acagctgttg cagtggtttc
    14401 gtgcaggttg tggtacaggc tcgcaattct gattaacgag ctgtccaggt agtatgaaga
    14461 aatacaataa gcaggaattt atcttatctc ccccgatttc cgtcatcgga ggagatataa
    14521 ctgtcaggca aatatcgtca tcaaataggc ggcaaacagt gccagatgcg ctgcgccatt
    14581 gagcacgtta gtacgtccgg tggagaagga gatatggcac agcactaaag aggccaccat
    14641 caccaccatt tctggcgcac caagtgcaaa ctgtaattcg ttacccgtca taaaggcaat
    14701 tagcgtgacg acaggtacgg taagcgaaat ggttgctaac actgaaccaa agaacagatt
    14761 catcgcgcgc tggacctggt tgttcaacac tgcttttaac gcacctaaac cttccggcga
    14821 cagaatcaac agagccacca ggaagccagt aaaggcgaca ggggcattca tgctgtcgag
    14881 caatgtctcc agcgggctgg cattcatttt ggtgactgca ataacggcaa tcagatggat
    14941 aatcaaccag atagcatgcc acacgctgct atgggcagac ggtttaccgt gatgcgggtc
    15001 atcgtcatca ctgtcatctt cgtgctcgta gacaaacaaa ctttgatgcg ttttggtctg
    15061 gatcagcaaa aatacgccat acattgccgc agaaattaat gctaccagta acgcctgacc
    15121 ggttgaaaaa ttcgccgcag gcagagccat tggaaatacc agtacgatta tcgccagggg
    15181 gaacagcgca attaaatact gcttgatacc aaacagattc atatattggg tggcaaactt
    15241 acgaccgccc aacaataatg aaaagccaac cagcccaccg gtaacaatca taatgattga
    15301 atagagcgta tcacgcatta gcgttggcgc ggcgtcgccg gttgccatta aagctgaaat
    15361 caaactgact tcaagaataa ccactgaaag gctaagaata agcgaaccgt aaggttctcc
    15421 caggcgatgg gctaatacgt ccgcatgacg gacaacacta aaggcgctac ttaaaatacc
    15481 aataagcgca agaagattga tggcaatgac cactggtagt gtctggctgc ttccccacag
    15541 gaacagcact accagcgcca gaaccgggaa aataagcgaa gtctccttgt ggcgggtttt
    15601 taccgcctct tgagcatttg acattatggt tatccctttg cagatgaatt tatcgaaaat
    15661 gtaaaaaata ggtaggaaaa ataacagaaa gtgtctggat atcggtaaca ttttacgaat
    15721 tt
//
LOCUS       DAZFVO020000118        15707 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFVO020000118 DAZFVO020000000
VERSION     DAZFVO020000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.119"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            202..444
                     /gene="yjbE"
                     /locus_tag="ACHKNB_RS17560"
                     /old_locus_tag="pgaptmp_003512"
     CDS             202..444
                     /gene="yjbE"
                     /locus_tag="ACHKNB_RS17560"
                     /old_locus_tag="pgaptmp_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HGU4196963.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            558..1196
                     /locus_tag="ACHKNB_RS17565"
                     /old_locus_tag="pgaptmp_003513"
     CDS             558..1196
                     /locus_tag="ACHKNB_RS17565"
                     /old_locus_tag="pgaptmp_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HGU4196964.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1193..1930
                     /locus_tag="ACHKNB_RS17570"
                     /old_locus_tag="pgaptmp_003514"
     CDS             1193..1930
                     /locus_tag="ACHKNB_RS17570"
                     /old_locus_tag="pgaptmp_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HGU4196965.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1930..4026
                     /locus_tag="ACHKNB_RS17575"
                     /old_locus_tag="pgaptmp_003515"
     CDS             1930..4026
                     /locus_tag="ACHKNB_RS17575"
                     /old_locus_tag="pgaptmp_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HGU4196966.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4073..4351)
                     /locus_tag="ACHKNB_RS17580"
                     /old_locus_tag="pgaptmp_003516"
     CDS             complement(4073..4351)
                     /locus_tag="ACHKNB_RS17580"
                     /old_locus_tag="pgaptmp_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196967.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4566..4976
                     /gene="psiE"
                     /locus_tag="ACHKNB_RS17585"
                     /old_locus_tag="pgaptmp_003517"
     CDS             4566..4976
                     /gene="psiE"
                     /locus_tag="ACHKNB_RS17585"
                     /old_locus_tag="pgaptmp_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HGU4196968.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5020..6495)
                     /gene="xylE"
                     /locus_tag="ACHKNB_RS17590"
                     /old_locus_tag="pgaptmp_003518"
     CDS             complement(5020..6495)
                     /gene="xylE"
                     /locus_tag="ACHKNB_RS17590"
                     /old_locus_tag="pgaptmp_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HGU4196969.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6867..7757)
                     /gene="malG"
                     /locus_tag="ACHKNB_RS17595"
                     /old_locus_tag="pgaptmp_003519"
     CDS             complement(6867..7757)
                     /gene="malG"
                     /locus_tag="ACHKNB_RS17595"
                     /old_locus_tag="pgaptmp_003519"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HGU4196970.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7772..9316)
                     /gene="malF"
                     /locus_tag="ACHKNB_RS17600"
                     /old_locus_tag="pgaptmp_003520"
     CDS             complement(7772..9316)
                     /gene="malF"
                     /locus_tag="ACHKNB_RS17600"
                     /old_locus_tag="pgaptmp_003520"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HGU4196971.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9470..10660)
                     /gene="malE"
                     /locus_tag="ACHKNB_RS17605"
                     /old_locus_tag="pgaptmp_003521"
     CDS             complement(9470..10660)
                     /gene="malE"
                     /locus_tag="ACHKNB_RS17605"
                     /old_locus_tag="pgaptmp_003521"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HGU4196972.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11025..12140
                     /gene="malK"
                     /locus_tag="ACHKNB_RS17610"
                     /old_locus_tag="pgaptmp_003522"
     CDS             11025..12140
                     /gene="malK"
                     /locus_tag="ACHKNB_RS17610"
                     /old_locus_tag="pgaptmp_003522"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HGU4196973.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12212..13552
                     /gene="lamB"
                     /locus_tag="ACHKNB_RS17615"
                     /old_locus_tag="pgaptmp_003523"
     CDS             12212..13552
                     /gene="lamB"
                     /locus_tag="ACHKNB_RS17615"
                     /old_locus_tag="pgaptmp_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HGU4196974.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13795..14715
                     /gene="malM"
                     /locus_tag="ACHKNB_RS17620"
                     /old_locus_tag="pgaptmp_003524"
     CDS             13795..14715
                     /gene="malM"
                     /locus_tag="ACHKNB_RS17620"
                     /old_locus_tag="pgaptmp_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HGU4196975.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14944..15707
                     /locus_tag="ACHKNB_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /pseudo
     CDS             14944..15707
                     /locus_tag="ACHKNB_RS17625"
                     /old_locus_tag="pgaptmp_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579094.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3958 a         3943 c         4057 g         3749 t
ORIGIN      
        1 cttttacttt tattttaaca ttatgatttt taatgttatt tatatataag ttgtagctta
       61 tatttgatat tcattccaat tatcctaaaa ccccatctct aattccccgc gccgtaattc
      121 gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc
      181 attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg
      241 cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agggtcggca
      301 gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg
      361 gtgggtgtcg cgctcgcggc aaccggtggc ggtgatggtt ctaataccgg gaccaccaca
      421 accacgacca ccagtaccca gtaataaagt atgtatcccc aaaataattt gagtcattgc
      481 atctgtggct tgaagtatga agggatttac cataaccaca ctccggtgtg gttattctgc
      541 ccctctggag aagagtcgtg aagcgacctg cactcattct tatctgcctg ctattacagg
      601 cctgttcagc cacgactaaa gagctgggca attcactgtg ggacagtctg ttcggcacgc
      661 caggcgtaca gctgacggac gatgatattc aaaatatgcc ctacgccagc cagtacatgc
      721 agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa
      781 aatgggtcac tcaggatcaa gccactcttg tcatacaaca tggtcgtctg gtgaagactt
      841 tgcttggcgg cgacaacctg attgaagtga ataaccttgc cgccgacccg ctgattaaac
      901 ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat gggctggacc gagtaccagc
      961 aggtacgcta cgccaccgca cgctcagtct tcaaatggga tggcaccgat accgtcaagg
     1021 tcggcagcga tgaaaccccg gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc
     1081 gctggcataa ccgctactgg atcgacagcg aagggcaaat tcgccagtcg gaacagtatc
     1141 tcggcgcgga ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa
     1201 acaaactatt gtcgcattga ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac
     1261 cgtcaaggta ttcagcaatg gcagcagtga ggccaaaacg ctgacgggcg cagagtattt
     1321 aatcgatctg gtaggccagc cgcggctggc aaacagttgg tggcctggtg cggtgattag
     1381 cgaagagctg gcaacggcgg cagcattgcg gcagcagcag gcgttgctga cacggctggc
     1441 agaactggct gcagattcca gcgccgacga tgccgccgcg attaacgcct tacgccagca
     1501 aattcaggcg ttgaaagtga cgggcaggca aaaaatcaat cttgatcccg atatcgtccg
     1561 cgttgccgaa cgcggtaacc caccgttgca gggcaactac acgctgtggg tcgggccacc
     1621 accgtccacg gtcacgctgt tcgggcttat cagccgtcct ggcaagcagc cattcactcc
     1681 aggtcgcgac gtggcgagct atctctccgg gcagaacctg cttagcggtg cggatcgcag
     1741 ctacgcgtgg gtggtttacc cggacggacg cacgcaaaaa gcgccggtgg cttactggaa
     1801 caagcgtcac gtagagccga tgcccggcag cattatttat gttggcctcg cggactccgt
     1861 ctggagtgag acgcctgatg cccttaacgc cgacattctt cagactctga cgcagcggat
     1921 accccaataa tgaaaaaaag acatctgctt agcttactgg cgctgggcat tagcacagct
     1981 tgctacggcg aaacatatcc tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta
     2041 ggattattac aaactcccac cgcgcgtatg gcacgggaag gggagttgag tctgaactat
     2101 cgcgataacg atcagtaccg ttattactca gcttcagtgc aactcttccc gtggctggaa
     2161 acaacgctgc gctacaccga cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct
     2221 ggcgatcaaa cgtataaaga taaagccttc gatctcaaac tgcgtttgtg ggaagagagt
     2281 tactggctgc cgcaagtggc ggttggtgcg cgggatatcg gcggtacggg gctgtttgat
     2341 gcggaatatc ttgttgccag caaagcctgg gggccgttcg attttacgct cggcctgggc
     2401 tggggatatc tgggcaccag cggtaatgta aaaaatccgc tctgttcagc cagtgataaa
     2461 tattgctatc gcgataacag ctacaaacag gcgggatcta tcgacggtag ccagatgttc
     2521 cacggtcctg cctcactgtt tggcggcgtg gaataccaga cgccctggca accgctgcgc
     2581 ctgaaactgg agtatgaagg caataattat cagcaggatt ttgccgggaa gctggagcaa
     2641 aaaagtaagt ttaacgtcgg tgcgatttat cgcgttaccg attgggccga cgttaacctt
     2701 agctatgaac gtggcaacac ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat
     2761 ctgcgcccgt cttacaacga taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc
     2821 attttgcagc attcggtggt ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt
     2881 gccgatccgc agatccaggc gaaaggcgat acgctgtatg ttaccggcga gcaggtgaaa
     2941 tatcgtgatt cgcgcgaagg gatcatccgt gctaatcgga tcgtgatgaa tgatctgccg
     3001 gatgggatca aaacgatccg cgttacggaa aatcgcctta acatgccgca ggtgacgacg
     3061 gaaaccgatg tcgccagcct gaaaaatcat ctcgccggag agccgttggg ccacgaaacg
     3121 acgctggcgc aaaaacgcgt cgagccagtg gttccgcagt ccaccgagca gggatggtat
     3181 atcgacaaat cacgctttga tttccatatc gatccggtgc tgaaccagtc ggtcggtggc
     3241 ccggaaaact tttacatgta tcagctgggc gtgatgggaa cggcagattt gtggctgacg
     3301 gaccatctgc tgacgaccgg cagcctgttt gcaaatcttg ccaacaacta cgacaagttt
     3361 aactacacta atcctccgca ggactcgcac ttaccgcgcg tgcgtaccca tgtgcgcgag
     3421 tatgtgcaga acgatgtcta tgtgaataac ctgcaagcca actacttcca gcatctgggc
     3481 aacggcttct acggtcaggt ctacggtggt tatctcgaaa ccatgtttgg cggtgcgggg
     3541 gcagaagtgt tgtatcgccc gctggacagc aactgggcgt ttggtctgga tgccaactac
     3601 gttaaacagc gcgactggcg tagtgcaaaa gatatgatga aattcaccga ctacagcgtg
     3661 aaaaccggac atctgaccgc ctactggacg ccatctttcg ctcaggatgt gttagttaaa
     3721 gccagcgtcg ggcagtatct ggcaggggat aaaggcggca cgctggagat cgccaaacgc
     3781 tttgatagcg gcgtggtggt gggtggctat gccacgatca ctaatgtttc aaaagaggag
     3841 tacggcgaag gggacttcac caaaggcgtg tatgtctcgg taccgttgga tctcttctcg
     3901 tctggcccga cacgcagccg tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt
     3961 cagcaacttg ggcgtaagtt ccagttgtat gacatgacca gcgaccgtag cgtcaatttc
     4021 cgctaagtca tgggaaaggt gccagttttc gcaatcactg gcaccctcat tcttaatggc
     4081 ctgttgagcc atcaggaacg cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa
     4141 aacattctgt cttcctgtcc gtcgatatcg gttgcagatc tggttgcctg agcgactggg
     4201 ccatcattgg gctggagaca ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg
     4261 gaccagcata agcaggaaga acagagactg ataacatcaa agcagcaaaa taaggcttca
     4321 tttttaccac ctttatcagg ttacgtttca tttgttccag aggaacatgg tcgatttttc
     4381 gcgcattgct ggtggctggg aatcacctga atgggtgatt ttttggggct gtgtgttttg
     4441 ccagatgcga cgttgatggg agatgcccac ttaatcaagc tgaacaaaac atacacaaaa
     4501 aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt gaaggaggta
     4561 ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt ttgcagaccg
     4621 tactcaatct tggcctgttg tgcctcggcc tgattttggt tgtcttcctc ggcaaagaaa
     4681 cggtgcatct ggcagatgtg ctgttcgcgc cagaacaaac cagcaaatat gagctggtag
     4741 aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg aaatactttc
     4801 agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc gccattgtgc
     4861 gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg gctgcgatcc
     4921 tgttactggt gatcactctg tggttgtgta attcgaaacg gctgaagcgg gagtaaaaag
     4981 acagcacgcc gaactggcgc ggcgtgctgg acaggaagat tacagcgtag cagtttgttg
     5041 tgttttcttc gtttccggtt cccagagggc ttccagctcc tcaagggttt tacctttggt
     5101 ttccgggaca aatttccaca taaatagtgc tgccagaacg cccatacacc cgtaaatcca
     5161 gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca tcatcgggaa
     5221 ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg ccagcgcttt
     5281 accacgaatg gcattcggga agatttccga cagcagaacc cagcataccg gaccccagga
     5341 catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac ccggtgcctg
     5401 agtgtaaaac gcggtaccga ggctaaacat accgattgcc attccgagcg cgccgataat
     5461 ttgcagtggc ttacgaccaa atttatccac cgtcataatt gccagaacgg tgaaggtgag
     5521 gttgataact ccgacaataa tggtctgcaa cagcgcgata tccgtgctgg cccccagcgt
     5581 tttgaacact tccggcgcgt agtacaacac cacattgatg ccgacaaatt gctggaagat
     5641 ggagagcata acgccgatta caatcacgcc cacgccaaac atcagcagac gaccaccggt
     5701 tttgcggcca tgatccaggg agtgtttaat ttcctgtact gcctgagttg caagcgtgtt
     5761 gcccataatt ttgcgcagga tactttccgc ctgttcttgc ttgccgcgtg acatcagcca
     5821 gcgaggactt tctggcacgg tatacagcag cattaagaac agcagtgcag ggatacattc
     5881 cgaggcaaac atataacgcc agccgtcagt attcagccag ctggcatcac cggaacgggc
     5941 aataaaatag tttacgcagt aaactaaaag ttgcccgaaa ataatcgcaa actggttaaa
     6001 tgaaaccagt tttccgcgaa tatgagctgg agccagttcc gcaatataca ttggtgaaag
     6061 cattgaggct aaaccaacgc caataccgcc aataatacga taaataacaa attccgggac
     6121 ataacctgcc agataaatag ggactgtgtt gtccgggttt atagaggtaa aaccaagttc
     6181 tggccaggca gaacctacac cagaaataaa aaacaggaca gcagcaatct taagtgaatc
     6241 acgacggccg aagcggttac tgcaataacc accgagggca ccgccgatga tgcaaccaat
     6301 cagagcgctg gctacgcaaa atcctaacag ggagttggca gcggattcac ttaagttttg
     6361 tggagcaaca aagacggtat tgagtgactc aacagtaccg gaaataacgg cggtgtcgta
     6421 gccaaataat aaaccaccta atgtagcgac taaggtaatc gaaaatatat aactggaatt
     6481 atactgggta ttcattcaga cctgccttag accattctga tcttttatca gtgcattgat
     6541 gttggaattg tcaggcagtt aaggcaatta tcatttttta caatgcgttt acgtaatgtc
     6601 ttttctgtga tcttaattgt gataattatc aaaaattgat gaaaacaata ctattgccgt
     6661 gactcagagc acgaaagaga attatcgtaa gtgggaaaac aaataacgta aaaataataa
     6721 gctctatgat gggaatatta accggcgaac gattcagatt gcagacgaaa gaaaaaaagg
     6781 cgctccgtgg agcgccgaat aacagtcaca agttgggata acgtaagttg agggtgcagc
     6841 ggcataacat tggcagaaca acatctttaa cctttcacac cacctgccgt caggccgttg
     6901 accagccagc gttgagccag caagaagacg atggtgatcg gtaatgcaga catcacggca
     6961 gcggcggcaa agtcacccca caggtagttt tgcgggttga ggtattgctg catccccacg
     7021 gccagggtgt agctgtttac gtcacgcagt aacagcgacg cgaccggaac ttcagtaatg
     7081 gcagcgataa acgacaggat gaataccacc gccagaatcg gtactgacag cggcaacagg
     7141 acaaggcgga aggcctgcca cggtgtcgca ccatccagcg cagccgcttc ttccagcgaa
     7201 ctgtcgatgg tttcgaaata gcctttgatg gtccagacat gcagcgcaat cccacccaga
     7261 tacgcgaaaa ttacgccgcc gtgagtattc aggccaatga atggaatgta ctcacccaga
     7321 cgatcaaaca acgcatacaa cgcgaccagt gaaagtactg ccgggaacat ctggaaaatc
     7381 agcattcctt tcagcagcgt cgctttgcct ggaaagcgca tacgggcgaa agcgtaggcg
     7441 caggtggtgg agagcgccac aatgccaatc gcggaaatcc cggcgacctt taccgagttc
     7501 cacagccaca gcagtaccgg gaatggcggt ggcgtaatgc gaccatcagc ctgttcaacg
     7561 ctaaaaccta acgccagttt ccagtgatcc caggagattt gctccgggat caggctaccg
     7621 gtcgcgaagt ttccctgacg cagcgagata gcgacgacca tcagcagcgg gaacataatc
     7681 gccgcgataa aaagtagcag tagcaggtga gtaataaaca aacgtgcttt ttgcgatttc
     7741 ggttggacca ttgccatttt tgttatctcc cttaatcaaa cttcattcgc gtggctttca
     7801 ggttcactat cgccagcgca cccaccagca ggaagatcag cgtggcaatt gctgcagcca
     7861 gaccgaagtc ctgacccccg ccgccttcaa aagcgatacg gtaggtgtag ttaacaagca
     7921 ggtcggtata accggctggc gtggtcgtgc caagacgatc cgggccgccg ttggttaaca
     7981 gctgaatcag cacgaagttg ttaaagttaa aggcgaagct ggcgatcatc agcggcgtca
     8041 gcggtttaat cagcagcggc agcgtaatct taaagaagtt ctggaacgga cctgcgccat
     8101 ccattgctga ggcttcatac aaatcgtccg gaatcgcttt cagcaagccc atgcagagga
     8161 tcatcatgta cggataaccc agccaggtat tgacgataat cagcatcgtt cgggcagtgg
     8221 tcggatcgct gaaccaggcg ggcttcacgc caaacagcgc gctcaacatc atgttgattt
     8281 caccgaagct ctggttaaac aaccctttga aaatcaagat tgaaatgaat gatggcaccg
     8341 cgtagggcag aatcagcagg acgcgatata ccgctttgcc acgcaacgct tcccactgca
     8401 ccagacacgc cagaaccatg ccgaccgcca ccgttaaaaa gacggtgatc agtgaaaaca
     8461 ccacggtcca gacgaaaatg gcgaggaacg gtttctgaat gccttcgtcg gtaaagacgc
     8521 gggtaaagtt tttccagccg gtggtcacgg tgtaaccggg gcttagcttt tcatcccccc
     8581 agttgccgtc ggcggtaatg gactggtaaa agccaatttg gttattcgga cgatatttca
     8641 cgccactctg attattcgtc aacgtgccgt taccgtcgag tgtgtagagc ggctgcgtgc
     8701 cagaaaactg gcgcagggag ctcatcatca ctttgttgcc atccggcaga atggcggtaa
     8761 tgtcactcag cgcctggcgg ttctgggtaa tcacgcgcag attcgcgcgt tcgccttcgg
     8821 gctgggcggt cgtttctttc agttgcagtt tttgctcgcc gccaaattta aacgcgtcgg
     8881 agaggtaatt tttaccggtt tcgccgtcgc tgagcgccag ttgccactca tcgcccgccg
     8941 ggtaaagacc aaagttatag gttttgcctg cttgccagga gcgatctaac aacacttcct
     9001 gcgcacgttc aaaagtcagc tggttagtgc tgctgtagtt ggtgaaggca atggcgatgg
     9061 tgcagaccag agggaagagg acgaataatc ccattccagc cattcccggg taaacatagc
     9121 gccaggcgta ggctttacga ttggcgaaaa tatacagccc cgctgaactc aatatcagcg
     9181 tggtaatggc gaacaggtat tccccttgtg cgtacattaa aacaacaagg taacccacca
     9241 gcaggccgag cagacctagc actgaccatt tcagcgcgtc gctttgccac caatgtttct
     9301 ttttaatgac atccatgggg ttcttcctca ttccaggacg gataaggctt tcacgcctta
     9361 tccgacaaca actgcctgat gcgacactga tgcgtcttat caggcctaca tgcgtttcag
     9421 ttttgtaggc cggacaaggc gttcacgccg catccggcat ttcatagcat tacttggtga
     9481 tacgagtctg cgcgtctttc agggcttcat cgacagtctg acgaccgctg gcggcgttga
     9541 tcaccgcagt acgcacggca taccagaaag cggacatctg cgggatgttc ggcatgattt
     9601 cacctttctg ggcgttttcc atggtggcgg caatacgtgg atctttcgcc aactcttcct
     9661 cgtaagactt cagcgccact gcacccagcg gtttgtcttt attgaccgct tccagacctt
     9721 cgtcagtcag cagatagttt tcgaggaact ctttcgccag ctctttgttc ggactggcgg
     9781 cgttaatacc ggcgctcagc acgccaacga acggtttaga tggttgaccc ttgaaggtcg
     9841 gcagtaccgt tacaccataa ttcactttgc tggtgtcgat gttggaccat gcccacgggc
     9901 cgttgatggt catcgctgtt tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg
     9961 tgtctgcatt catgtgtttg tttttaataa ggtcaaccag gaaggtcaga cccgctttcg
    10021 cgccagcgtt atccacgccc acgtctttaa tatcgtactt gccgttttca tacttgaacg
    10081 cataaccccc gtcagcagca atcagcggcc aggtgaagta cggttcttgc aggttgaaca
    10141 tcagcgcgct cttacctttc gctttcagtt ctttatccag cgccgggatc tcttcccagg
    10201 tttttggtgg gttcggcagc agatctttgt tataaatcag cgataacgct tcaacagcga
    10261 tcgggtaagc aatcagcttg ccgttgtaac gtacggcatc ccaggtaaac ggatacagct
    10321 tgtcctggaa cgctttgtcc ggggtgattt cagccaacag gccagattga gcgtagccac
    10381 caaagcggtc gtgtgcccag aagataatgt cagggccatc gccagttgcc gcaacctgag
    10441 ggaatttctc ttccagttta tccggatgct caacggtgac tttaattccg gtatctttct
    10501 cgaatttctt accgacttca gcgagaccgt tatagccttt atcgccgtta atccagatta
    10561 ccagtttacc ttcttcgatt ttggcgagag ccgaggcgga aaacatcatc gtcgttaatg
    10621 cggataatgc gaggatgcgt gcacctgttt ttattttcat aatctatggt ccttgttggt
    10681 gaagtgctcg tgaaaacacc taaacggact ctagtttctt tatacggcaa cctctttcca
    10741 tcctccttgc ccctacgccc caccgttgct ttgtgtgatc tctgttacag aattggcggt
    10801 aatgtggaga tgcgcacata aaatcgccac gatttttgca agcaacatca cgaaattcct
    10861 tacatgacct cggtttagtt cacagaagcc gtgttctcat cctcccgcct cctcccccat
    10921 aaaaaagcca gggggtggag gatttaagcc atctcctgat gacgcatagt cagcccatca
    10981 tgaatgttgc tgtcgatgac aggttgttac aaagggagaa gggcatggcg agcgtacagc
    11041 tgcaaaatgt aacgaaagcc tggggcgagg tcgtggtatc gaaagatatc aatctcgata
    11101 tccatgaagg tgaattcgtg gtgtttgtcg gaccgtctgg ctgcggtaaa tcgactttac
    11161 tgcgcatgat tgccgggctt gagacgatca ccagcggcga cctgttcatc ggtgagaaac
    11221 ggatgaatga cactccgcca gcagaacgcg gcgttggtat ggtgtttcag tcttacgcgc
    11281 tctatcccca cctgtcagta gcagaaaata tgtcatttgg cctgaaactg gcaggcgcaa
    11341 aaaaagaggt gattaaccaa cgcgttaacc aggtggcgga agtgctgcaa ctggcgcatt
    11401 tgctggatcg caaaccgaaa gcgctctctg gcggtcagcg tcagcgtgtg gcgattggcc
    11461 gtacgctggt ggccgagcca agcgtatttt tgctcgatga accgctctcc aacctcgatg
    11521 ctgcactgcg tgtgcaaatg cgtatcgaaa tctcccgtct gcataaacgc ctgggccgca
    11581 caatgattta cgtcacccac gatcaggtcg aagcgatgac gctggccgac aaaatcgtgg
    11641 tgctggacgc cggtcgcgtg gcgcaggttg ggaaaccgct ggagctgtac cactatccgg
    11701 cagaccgttt tgtcgccgga tttatcggtt cgccaaagat gaacttcctg ccggtaaaag
    11761 tgaccgccac cgcaatcgat caagtgcagg tggagctgcc gatgccaaat cgtcagcaag
    11821 tctggctgcc agttgaaagc cgtgatgtcc aggttggagc caatatgtcg ctgggtattc
    11881 gcccggaaca tctactgccg agtgatatcg ctgacgtcat ccttgagggt gaagttcagg
    11941 tcgtcgagca actcggcaac gaaacccaaa tccatatcca gatcccttcc attcgtcaaa
    12001 acctggtgta ccgccagaac gacgtggtgt tggtagaaga aggtgccaca ttcgctatcg
    12061 gcctgccgcc agagcgttgc catctgttcc gtgaggatgg cactgcatgt cgtcgactgc
    12121 ataaggagcc gggcgtttaa gcaccccaca aaacacacaa agcctgtcac aggtgatgtg
    12181 aaaaaagaaa agcaatgact caggagatag aatgatgatt actctgcgca aacttcctct
    12241 ggcggttgcc gtcgcagcgg gcgtaatgtc tgctcaggca atggctgttg atttccacgg
    12301 ctatgcacgt tccggtattg gctggacagg tagcggcggt gaacaacagt gtttccagac
    12361 taccggtgct caaagtaaat accgtcttgg caacgaatgt gaaacttatg ctgaattaaa
    12421 attgggtcag gaagtgtgga aagagggcga taagagcttc tatttcgaca ctaacgtggc
    12481 ctattccgtc gcgcaacaga atgactggga agctaccgat ccggccttcc gtgaagcaaa
    12541 cgtgcagggt aaaaacctga tcgaatggct gccaggttcc accatctggg caggtaagcg
    12601 cttctaccaa cgtcatgacg ttcatatgat cgacttctac tactgggata tttctggtcc
    12661 tggtgccggt ctggaaaaca tcgatgttgg cttcggtaaa ctctctctgg cagcaacccg
    12721 ctcctctgaa gctggtggtt cttcctcttt tgccagcaac aatatttatg actataccaa
    12781 cgaaaccgcg aacgacgttt tcgatgtgcg tttagcgcag atggaaatca acccgggcgg
    12841 cacattagaa ctgggtgtcg actacggtcg tgccaacctg cgtgataact atcgtctggt
    12901 tgatggcgca tcgaaagacg gctggttatt cactgctgaa catactcaga gtgtcctgaa
    12961 gggctttaac aagtttgttg ttcagtacgc tactgactcg atgacctcgc agggtaaagg
    13021 tctgtcgcag ggttctggcg tcgcgtttga taacgaaaaa tttgcctaca atatcaacaa
    13081 caacggtcac atgctgcgta tcctcgacca cggtgcgatc tccatgggcg acaactggga
    13141 catgatgtac gtgggtatgt accaggatat caactgggat aacgacaacg gcaccaagtg
    13201 gtggaccgtt ggtattcgcc cgatgtacaa gtggacgcca atcatgagca ccgtgatgga
    13261 aatcggctac gacaacgtcg aatcccagcg caccggcgac aagaacaatc agtacaaaat
    13321 taccctcgca caacaatggc aggctggcga cagcatctgg tcacgcccgg ctattcgtgt
    13381 cttcgcaacc tacgccaagt gggatgagaa atggggttat gactacaacg gcgatagcaa
    13441 ggttaacccg aactacggca aagccgttcc tgctgatttc aacggcggca gcttcggtcg
    13501 tggcgacagc gacgagtgga ccttcggtgc ccagatggaa atctggtggt aatagcaaaa
    13561 cctgggccgg ataaggcgtt tacgccgcat tcggcaacca acgcctgatg cgacgcttgc
    13621 gcgtcttatc aggcctacaa cggctgtcaa atgtaggccg gataaggcgt ttacgccgca
    13681 tccggcataa aaacaggttg tcattatctg aaaggggcga aagcccctct gattatcggg
    13741 tttagcgcgc tattgcctgg ctaccgctga gctccagatt ttgaggtgaa aacaatgaaa
    13801 atgaataaaa gtctcatcgc cctctgttta tcagcagggt tactggcaag cgcgcctgga
    13861 atcagccttg ccgatgttaa ctacgtaccg caaaacacca gcgacgcgcc agccattcca
    13921 tctgccgcgc tgcaacaact cacctggaca ccggtcgatc aatctaaaac ccagaccacc
    13981 caactggcga ccggcggcca acaactgaac gttcccggca tcagtggtcc ggttgctgcg
    14041 tacagcgtcc cggcaaacat tggcgaactg accctgacgc tgaccagcga agtgaacaaa
    14101 caaaccagcg tttttgcgcc gaacgtgctg attcttgatc agaacatgac cccatcagcc
    14161 ttcttcccca gcagttattt cacctaccag gaaccaggcg tgatgagtgc agatcggctg
    14221 gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa aactttatgt tctggtcttt
    14281 accacggaaa aagatctcca gcagacgacc caactgctcg acccggctaa agcctatgcc
    14341 aagggcgtcg gtaactcgat cccggatatc cccgatccgg ttgctcgtca taccaccgat
    14401 ggcttactga aactgaaagt gaaaacgaac tccagctcca gcgtgttggt aggaccttta
    14461 tttggttctt ccgctccagc tccggttacg gtaggtaaca cggcggcacc agctgtggct
    14521 gcacccgctc cggcaccggt gaagaaaagc gagccgatgc tcaacgacac ggaaagttat
    14581 tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc gttaaaactg
    14641 cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt ccacctttat cagcagtgta
    14701 aaaggcaagg ggtaattacg ccccacagtg ctgattttgc aacaactggt gcgtctcctg
    14761 gcgcaccttt ttttatgctt ccttcctggg atatgagcga ttttttatag taactcactt
    14821 cttcttcact aagaatatcc attatctcaa tgccttatca gagattcttt tcctttcgcc
    14881 ggtagtgtct ggacattcag gctacttttc catgttattt tatttctgtt atgcagaggt
    14941 tttatgataa gtcatatccc aaattctggc ggcaataact ctttgatgaa acatgatgtg
    15001 gtgcaaggaa ataatatagt agatcttgat ttactacgta atttcaatgg ggtgccaggt
    15061 ttaaacagag ataactttat ttatatcagc aatatttttt caaatataaa acaacggaac
    15121 gaaaaaatca tgcaataaat ttgtttcgtg aagtctcaat cagtaatgat actataagtg
    15181 taaaattcta cagaaatgaa aaaaattgaa tgcgcttgca attttctgat ggataaagat
    15241 gcgcaggggt atatcgacct gtctgatttg gatttaacaa gttgtcattt taaaggtgac
    15301 gttatatcga aggtgtcttt tttatcatca aatctacaac atgtaacatt cgaatgtaaa
    15361 gaaattgggg attccaattt tactactgca atagttgata atgtcatatt tagatgtcga
    15421 cgtttacaca atgtgatttt tatcaaagcg agtggtgaat gtgtcgattt cagcaaaaat
    15481 attcttgata cagttgactt ctcgcagagt caacttactc agagtaattt tcgcgaatgt
    15541 cagattagaa attcaaactt cgataattgt tatctttacg cttcgcactt caccagagca
    15601 gagtttctgt ctgccaaaga aatatcattt attaaatcga atatgacagc tgttatgttt
    15661 gatcatgtgc gaatatcgac agggaatttt aaagattgca ttacaga
//
LOCUS       DAZFVO020000119        15683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFVO020000119 DAZFVO020000000
VERSION     DAZFVO020000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.120"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..167)
                     /locus_tag="ACHKNB_RS17630"
                     /old_locus_tag="pgaptmp_003526"
     CDS             complement(<1..167)
                     /locus_tag="ACHKNB_RS17630"
                     /old_locus_tag="pgaptmp_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4196976.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQI"
     gene            335..919
                     /locus_tag="ACHKNB_RS17635"
                     /old_locus_tag="pgaptmp_003527"
     CDS             335..919
                     /locus_tag="ACHKNB_RS17635"
                     /old_locus_tag="pgaptmp_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196977.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVNGYGDEPSMKKEPIEQTKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            1193..1828
                     /locus_tag="ACHKNB_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /pseudo
     CDS             1193..1828
                     /locus_tag="ACHKNB_RS17640"
                     /old_locus_tag="pgaptmp_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            1825..2913
                     /locus_tag="ACHKNB_RS17645"
                     /old_locus_tag="pgaptmp_003529"
     CDS             1825..2913
                     /locus_tag="ACHKNB_RS17645"
                     /old_locus_tag="pgaptmp_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HGU4196978.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3001..4299)
                     /gene="exuT"
                     /locus_tag="ACHKNB_RS17650"
                     /old_locus_tag="pgaptmp_003530"
     CDS             complement(3001..4299)
                     /gene="exuT"
                     /locus_tag="ACHKNB_RS17650"
                     /old_locus_tag="pgaptmp_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HGU4196979.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4782..6194
                     /gene="uxaC"
                     /locus_tag="ACHKNB_RS17655"
                     /old_locus_tag="pgaptmp_003531"
     CDS             4782..6194
                     /gene="uxaC"
                     /locus_tag="ACHKNB_RS17655"
                     /old_locus_tag="pgaptmp_003531"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HGU4196980.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6209..7696
                     /gene="uxaA"
                     /locus_tag="ACHKNB_RS17660"
                     /old_locus_tag="pgaptmp_003532"
     CDS             6209..7696
                     /gene="uxaA"
                     /locus_tag="ACHKNB_RS17660"
                     /old_locus_tag="pgaptmp_003532"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HGU4196981.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7779..8330
                     /gene="ygjV"
                     /locus_tag="ACHKNB_RS17665"
                     /old_locus_tag="pgaptmp_003533"
     CDS             7779..8330
                     /gene="ygjV"
                     /locus_tag="ACHKNB_RS17665"
                     /old_locus_tag="pgaptmp_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HGU4196982.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(8335..9579)
                     /gene="sstT"
                     /locus_tag="ACHKNB_RS17670"
                     /old_locus_tag="pgaptmp_003534"
     CDS             complement(8335..9579)
                     /gene="sstT"
                     /locus_tag="ACHKNB_RS17670"
                     /old_locus_tag="pgaptmp_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HGU4196983.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10195..11160)
                     /gene="alx"
                     /locus_tag="ACHKNB_RS17675"
                     /old_locus_tag="pgaptmp_003535"
     CDS             complement(10195..11160)
                     /gene="alx"
                     /locus_tag="ACHKNB_RS17675"
                     /old_locus_tag="pgaptmp_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HGU4196984.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11443..12429)
                     /gene="ygjR"
                     /locus_tag="ACHKNB_RS17680"
                     /old_locus_tag="pgaptmp_003536"
     CDS             complement(11443..12429)
                     /gene="ygjR"
                     /locus_tag="ACHKNB_RS17680"
                     /old_locus_tag="pgaptmp_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGU4196985.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(12508..13191)
                     /gene="ygjQ"
                     /locus_tag="ACHKNB_RS17685"
                     /old_locus_tag="pgaptmp_003537"
     CDS             complement(12508..13191)
                     /gene="ygjQ"
                     /locus_tag="ACHKNB_RS17685"
                     /old_locus_tag="pgaptmp_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HGU4196986.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(13268..13771)
                     /gene="ygjP"
                     /locus_tag="ACHKNB_RS17690"
                     /old_locus_tag="pgaptmp_003538"
     CDS             complement(13268..13771)
                     /gene="ygjP"
                     /locus_tag="ACHKNB_RS17690"
                     /old_locus_tag="pgaptmp_003538"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HGU4196987.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            13856..14992
                     /gene="rlmG"
                     /locus_tag="ACHKNB_RS17695"
                     /old_locus_tag="pgaptmp_003539"
     CDS             13856..14992
                     /gene="rlmG"
                     /locus_tag="ACHKNB_RS17695"
                     /old_locus_tag="pgaptmp_003539"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HGU4196988.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            15183..15266
                     /locus_tag="ACHKNB_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /pseudo
     CDS             15183..15266
                     /locus_tag="ACHKNB_RS17700"
                     /old_locus_tag="pgaptmp_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            15263..15679
                     /gene="higA"
                     /locus_tag="ACHKNB_RS17705"
                     /old_locus_tag="pgaptmp_003541"
     CDS             15263..15679
                     /gene="higA"
                     /locus_tag="ACHKNB_RS17705"
                     /old_locus_tag="pgaptmp_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HGU4196989.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
BASE COUNT         4129 a         3967 c         4001 g         3586 t
ORIGIN      
        1 attatttgct gatacggtcg gcaaggagta cgtaatacgc ttttcagcga atccctgctg
       61 taaccagcct ttaaagataa agagcgcagt gataagccag ccatgtgtaa gcgcgccttg
      121 atgatttcag gcggccaatc gtgatctgaa acttctcttc tactcatact tcctctattc
      181 taaaaagtta cccgcgcgga tattcgtgcg gatatctgta cgggtaaata atagaacaaa
      241 caaaagaacc aaatcaaggt attttgttct ttttattctt tgtggtgttt ttgttattaa
      301 tatcaataag ataaatacaa aggaacctga gagcatgaac atgaaaaaca gttcttttct
      361 cgacgaagga aaagaaccag tgatcgaacg gatttttcgt cttgcagagc gttacaaatc
      421 gagaagtgag gcggctagag catggggaat aaacgttcag acattgcaga actactacaa
      481 aaggagaaac aatcagccgc caccacaacc aagaaagcac catctccaga acattgcaga
      541 aagagaacat gtatctatgg agtggctagt caatggttat ggtgatgagc caagcatgaa
      601 aaaagaacca attgaacaaa caaaaagaac aaaaaaacaa agaattgatg atagtgattc
      661 catcatcatg agggcgtggg actcgctaac ttcatctgaa caagagattt tgtccaactt
      721 attgatcaga aaaggggccg aactactaac cgttttgctt gatacagata ttcaaaaact
      781 gcactcatta agcggagtca aaagagcact agcattgagt ctacaagagt tacccgatga
      841 tactgtaaga gagatttatg aagaatgtga agctaaggtt aatcatttaa atgtaaccca
      901 gaaaaaggct ggtgcgtaag ggagtggtgc aatttttgtg ctgtagtaca ctcctttaaa
      961 gacaatctgg agaaaaacgt tttaagcctt tttaaagcca ttttaagcgt caatccgtag
     1021 tatcaaaagt tttgccgatt ttagaataaa cgattaatta ttgcacaatc ggtatcaaat
     1081 agactttaag cgctcatcca gacatcacct gttttatttc agtatcttac aggtgatttc
     1141 acctcctttc actaaaaccc tcgttggtat caaatgattc acctggttac atggtacaga
     1201 cgatttgctg ggtgcggtac tggtggatgt gaaaggcttc tctgaacagg atgaagagag
     1261 tcaggatctg caactcgaag cgcgggtggc aggcagccga actttgcagc ttggtcaaag
     1321 tgacagtgtg ttgttccctt atcctggatt tcagtctggt tttgttgagg ttaacgacag
     1381 tagccagggc aatcagcagg ggacaacaaa catcattaac ggtgcaggga atcgtgaatt
     1441 aatgttgttg cctggcaagc tgcgctatcg cgaagtgtct gccagcttta actacaacta
     1501 tatcggtcgt ttattactgc cggcagcggt gaaaaaattc ccgattgtgg ggctgaacag
     1561 cgccatgtta ctggtagctg aagatggcgg atttacactt gaaattaacg gcagcgaaaa
     1621 agagctctat ctgctttccg ggcagcaatt ccttaagtgt ccactgagtg ttgtaaagaa
     1681 acgcgccagc attcgttaca gcggagatgt tacttgtagt gtggtgactt attcacaatt
     1741 accggagtcg attcaggttc aggcacagtt gaaacagcct aaattacgtg gaaacgttca
     1801 gacggcgcaa agggaggttg caccatgaga aatcgattga ttgcggcgat attgggcttg
     1861 tttggcacgc tcactggcgt tcaggcagct cctgacgtga ccagtgaaat tacgtatgat
     1921 ttggcatctg gtagagcgga ttattacttc tggaaggatg aagcgtcggc aggaaataat
     1981 ggatatatgt ggtatgaatg ttcgtatcct gacctccaac aaacctgtac agctaatgga
     2041 aatatatcga cagtacaaat ctatttaact gaacaacgca gtgggatgcg ttggccggta
     2101 aaactcaaag gatttaaaac agccattgta agtagcgatg aagcgccgcc aggatgcaag
     2161 gggggcaaag ggcttcagac gaatcttaag gattctaata gatcttcatg tacagaagat
     2221 ggtcaacatt attatatata cgatacaaag tttcttacgc tctaccttga gcagacagag
     2281 atgaagaatt tgccgattgg tggcgtctgg aaggggaaag ttaaattaca ttcgaacagc
     2341 ccggcccagg actatttcgc aaatattacc ctgaatacgc tcgaccccaa ccatattgac
     2401 gtgttcttcc cggagttcgc ccacgccacg ccaagggtgc agttagactt gcatccaaca
     2461 ggaagcgtta acggcagcaa ctacgcgcaa gatctgacca tgttggacat gtgcctgtac
     2521 gatggtttta acggtaatgc catcagttat gaaatcatgc tcaaagatga agggcgacca
     2581 gctgcagggc gcagagacgg ttacttctct atctatcgtc agggagggac caccaccgac
     2641 gagggagaac gcattgatta ccgggtcaaa atgtacaacc cggaaaccgg tgggcaaatt
     2701 gatgtgcgca ataatgaaaa tatggtctgg aacagcatta acctgaaacg tgtgcgtccg
     2761 gtggtactgc ccggtattcg ctatgccgtg atgtgtgtgc cgacaccctt gactctggca
     2821 gtcgataaat tcagcgtgat ggataaacag gccgggtact acatgggcaa attgtcagta
     2881 atctttacgc cttccttgcc aaccatcaat taaccctgca aaccggacaa acgctttttt
     2941 ctatataaac gacgaagccg tctgataagg cggcttcgtc attacaactt aagttaaacg
     3001 ttaatgttgc ggtgcgggat cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt
     3061 ctgcaacacg gtccagataa ccagcgcacc taacaggtca aatactgcca gaactgcgaa
     3121 cagcgggctg aagccgatgg tgtcagccag tgcaccgaca accagcgcaa acagcgtact
     3181 tgccagccat gcggacatcc cggttaaacc gttcgccgtc gccacttcgt tacgaccgaa
     3241 cacgtcagaa gagagcgtaa tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat
     3301 acacagcagc ataattgcga catacgggtt ggtgaacaga ccgatcatac ccgggccaat
     3361 catcagcaca gcacccagcg ttacgaccat cttacgggaa acgatcaggt tcacaccaaa
     3421 ccaacgctgg aacagcggcg gcaggtaacc accgaggata caaccgaggt cagcaaacag
     3481 catcggcatc caggcgaaca tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat
     3541 gaacagcggg atccacgcgt taaaagtacc ccaggccggt tctgccagga aacgcggcag
     3601 cgcgataccc cagaactgac ggttacgcag gatctgacca acggacattt tcttcgccgt
     3661 gctcacctgg tgctgggctt cctgaccatt aataatatag tcgcgttctt catcggtcag
     3721 atgcttctgg tcgcgtggat gtttatagaa aatcagccat gccatcgccc agataaagct
     3781 caatgcaccg gagatgataa atgccatctg ccagctgtgc attacgattg cccataccac
     3841 cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtaac ctaccgcgat
     3901 ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg ggatcatcgc
     3961 tgcttccgcg gcaccgactg caccacgagc aatcgccagg ccaccccagc taccagccag
     4021 cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac cgattttcgt
     4081 acccagcaca tccagtacat agcccgctac cggttgcatg accgtataag cagcagaata
     4141 ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca tcagagttgg
     4201 cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccaa gcgtcaccag
     4261 tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca tcgttttgtt
     4321 atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag ttacgaccgg
     4381 gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt gctaattcgg
     4441 cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat ttgcattccg
     4501 tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa aaggtgagag
     4561 ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag gccagtactg
     4621 gcgcggcctg cagagagatt taccactttg agagtaattt ttttaactac gtttattgat
     4681 ctaactcacg aaaatatctt cggactctgg aaattggtgt gataactttg tcagcatcgc
     4741 accataagca agctagctca ctcgttgaga ggaagacgaa aatgactccg tttatgactg
     4801 aagatttcct gttagatacc gaatttgccc gccgtctgta tcacgactac gcaaaagacc
     4861 agccgatttt cgattaccat tgccatttgc cgccgcagca gattgcggaa gactatcgtt
     4921 ttaaaaacct gtatgacatc tggctgaaag gcgatcacta caaatggcgc gctatgcgta
     4981 ccaacggtgt ggccgagcgt ctgtgtaccg gtgatgcgtc tgaccgtgaa aaatttgacg
     5041 cctgggcggc gactgttccg cacactatcg gcaacccgtt ataccactgg acgcacctcg
     5101 aactgcgtcg tccgtttggt atcactggca aattgctttc tccgtcaact gccgatgaaa
     5161 tctggaacga atgtaacgaa ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc
     5221 agcagatgaa cgtgaaaatg gtcggcacca ccgatgaccc gatcgattct ctggagcatc
     5281 acgcagagat cgccaaagac ggctctttca ccatcaaagt gctgccgagc tggcgtccgg
     5341 acaaagcctt caacatcgaa caggcgacct ttaacgacta catggcgaag ctgggcgaag
     5401 tttccgatac cgacattcgc cgctttgctg acctgcaaac tgccttgacc aaacgtctgg
     5461 atcacttcgc cgctcacggc tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg
     5521 ctgaagcgaa cgaagcggaa ctggacagca ttctggcgcg ccgtctggct ggcgaacccc
     5581 tgagcgagca cgaagtggca cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat
     5641 atgcacgtcg cggctgggta cagcagtacc acattggcgc gctgcgtaat aacaacctgc
     5701 gtcagttcaa actgctgggg ccggatgtag gctttgactc catcaacgac cgtccgatgg
     5761 cggaagagtt gtctaagctg ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca
     5821 ttctctactg cctgaacccg cgcgataacg aagtgctggg caccatgatc ggtaacttcc
     5881 agggcgaagg tatgccgggc aaaatgcagt tcggttccgg ctggtggttt aacgatcaga
     5941 aagacggtat ggaacgtcag atgacccaac tggcgcagct cggtctgctg agccgctttg
     6001 tcggtatgct gactgacagc cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc
     6061 gcattctgtg ccagatgatc ggtcgctggg tggaagcggg cgaagcaccg gcggacatca
     6121 acctgctggg cgagatggtg aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca
     6181 ttgaactgaa ctaaggtctg gggttgatat gcaatacatc aagatccatg cgctggataa
     6241 cgtcgcggtc gctttagcag atttggctga aggcacagaa gtcagtgtcg ataaccagac
     6301 tgttacgctg cgccaggatg ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa
     6361 aggggccaac gtcatcaaat atggcctgcc gattggttat gcattggcgg atattgcggc
     6421 aggtgttcac gttcacgccc acaatacgcg cacgaatctg agcgatctgg atcagtatcg
     6481 ctatcaacct gattttcagg atctgcctgc gcaagcggca gatcgtgaag tgcagatcta
     6541 tcgtcgcgct aacggcgatg tcggggtgcg taatgagctg tggatcctgc caaccgtggg
     6601 ctgtgtcaac ggcatcgcgc ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc
     6661 cgaaggtacc gacggcgtgt tcctcttcag ccacacctac ggctgctcac aactgggcga
     6721 cgatcacatc aacacccgca ccatgctgca aaacatggtg cgccacccga acgcgggcgc
     6781 agtgctggtg attggtctgg gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt
     6841 gggcgatatc gatcctgaac gcgttcattt catgatctgc caacagcagg atgatgagat
     6901 cgaagccggg attgagcatt tgcatcagct gtataacgtg atgcgcaacg ataaacgcga
     6961 gccaggcaaa ctcagcgaac tgaagtttgg tctggagtgc ggtggttctg acggtctttc
     7021 tggtattact gctaacccga tgctggggcg tttctctgac tacgtgattg ctaacggcgg
     7081 tactaccgta ctgaccgaag tgccggagat gtttggcgca gagcagttgc tgatggacca
     7141 ttgccgcgac gaagcaacgt ttgaaaaact ggtcaccatg gtcaacgact tcaaacagta
     7201 ctttattgcc catgaccagc cgatctatga aaacccatcg ccggggaaca aagcgggcgg
     7261 tatcaccacg ctggaagata aatcccttgg ctgtacccag aaagcgggtt ccagcgtcgt
     7321 ggttgacgtg ctgcgttacg gcgagcgtct gaaaacgccg gggctgaact tgttaagtgc
     7381 gccgggtaac gatgccgtag cgaccagcgc cctggcgggt gcgggctgcc atatggtgct
     7441 gttcagtact ggtcgtggca cgccatacgg cggctttgta ccgacggtga aaatcgctac
     7501 caacagtgaa ctggcggcga agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat
     7561 ccacggtaaa gcgatgccgc agttgctgga agaatttatc gacaccatcg ttgagtttgc
     7621 caacggtaaa caaacctgca acgagcgtaa cgacttccgc gaactggcga tcttcaaaag
     7681 cggcgtaacg ctataaagtc gaaatgagtt gttaataaaa aacggcgttt cataatgtga
     7741 agcgccgttt gcttttccat acctgataag gatttatcat gaccgcgtat tggctggccc
     7801 agggcgtggg tgtcatcgcc tttctgattg gtatcacaac atttttcaat cgtgacgaac
     7861 gtcgcttcaa aaagcagctt tcggtctata gcgccgttat tggcgtacat ttttttcttc
     7921 tgggcaccta tcccgctggt gccagtgcca tccttaatgc cattcgtaca ttgattacct
     7981 tacgcacgcg cagcttatgg gtaatggcga tttttattgt gctgactggc ggaattggcc
     8041 tcgcgaagtt ccatcatcct gtcgaactat tgccggttat cggtacgatt gtcagtacct
     8101 gggcgctgtt ccgctgtaaa gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt
     8161 gctgggtgat tcacaacttc tgggcggggt cgataggcgg cacaatgatt gagggtagtt
     8221 ttctgcttat gaatggcttg aatatcattc gtttctggcg gatgcagaaa aggggaattg
     8281 atccgtttaa agtagagaaa acccctcccg ccgtagacga aaggggttaa acaattaatt
     8341 acgcagggcg ctatttgcca gacgatcgtc ttccgcctgg caagctgccg cagtgaacag
     8401 cacgtcagtt gaagagttca gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa
     8461 gccgacggca accacctgca tggcgatatc gttcgaaata ccgaacatat tacaggccag
     8521 cgggatcagc agcagagaac cccccgccac gccggatgcg ccacaggcac acagagaagc
     8581 caccacgctc aacagcagcg ccgtgggcag atcgaccggg atacccagcg tattaaccgc
     8641 agccagcgtc aacacggtaa tggtgattgc tgcgcccgcc atattgatgg tggcacccag
     8701 cggaatagaa acggaatagg tatcgcgatc cagattcagc ttttcacaca gtgccatatt
     8761 caccggaatg ttcgccgcag agctgcgggt gaagaaggca tacacgccgc tttcgcgcag
     8821 gcacagcagc accagcggga acgggttacg acgaattttc caccacacca gcaatgggtt
     8881 aaccaccagc gccaccagta acatacagcc aaccagcacg accagcagtt gcgcgtagcc
     8941 ccacagtgtg gagaaaccgg tggttgccag ggtagaagaa accagaccaa aaataccaat
     9001 cggtgcgaag cgaatgacca gtttcaccat aaaggtgacg gcattcgaca tatcgttgac
     9061 caggtttttg gtggtctcgt taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac
     9121 caggatcccg atgtagttac ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat
     9181 taccagcccg cgcatcactt cgacaatgcc tgacggcggc gaaatatcac ccgcgctact
     9241 ggataaatgc agggtagaag ggaaggcaaa gctgaagact actgcggcca gagcagcaga
     9301 gaaggtaccc agcagataga ggaacaaaat agggcggata ttggttttct gcccgtgctg
     9361 gtggttagca atagatgcca tcaccagcat caacaccagg atgggggcaa cggctttcag
     9421 tgcgccgacg aacaaagtac ctaacagacc aacagcttcc gccgcgggtt ttgagatcca
     9481 tgccagaaga atccccagaa caaggccgac caggatttgt tttaccaggc tgccatgagc
     9541 cagacgccgg aatagccccg gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt
     9601 tgtcgcacat ccctggtgcg ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc
     9661 aaggtcgagt ataaggaaag ttacagcttc gggaagcgaa aaatgctgga ttttaaagag
     9721 aggtcatatt ttgcaactta attgttacat ttgtttgaca taaacagagg gaaggactgg
     9781 ctactaaggc tgattagata acatatgggc aaaactattt ggaatatgag aacggttgcg
     9841 ctcctttaat gtgttatgcc atataaagtc actggcgcag ggagcgcgca gggggcggcc
     9901 aatcgccgcc gccccatgca cccccgggct ctggcgaaca aaatcgccgc tgcgcggttc
     9961 cctcagctta tcccttgcgg ctaccgggtc gggcgcaagg taacgtccct gtaagatgcg
    10021 ccctcgccgc gcatccatgc gcgtcgcccc ggcctccagg gaacgcttcg gcgattttga
    10081 cgccaccaac acccgtgcgg tctttttgta aagagctata agccgattac ataattaata
    10141 gctcaccttt gtctggaatg agttatctct agtaatggct taatatacag cggatcattc
    10201 tccccgctgc ttatcatgcc gataattcac ccaggcgttg ataataaacg tcatcaccag
    10261 aatgccaaac accacgccca gcgagacggc gattggaata tggtagaagt cgacaatcag
    10321 catcttgata ccgataaaca ccagaatcac cgccaggcca tatttgagca tcgagaaacg
    10381 ctctgctacg cccgccagca ggaaatacat cgcacgcagg ccgaggatcg caaacaggtt
    10441 tgaggtcagc acaatgaacg ggtcagtggt cacggcgaag atagccggaa tgctatccac
    10501 ggcgaaaatc acgtcgctca attccaccag aatcagtacc agcatcagcg gtgtggcgta
    10561 cagcaatcca ttcttacgca caaagaaatg ctcgttgtcg atggtgtcgg tcatgcgcaa
    10621 atgaccgcgt agccagcgca ccagcggctt gtcgccaatg cctgattcat cttcatgggc
    10681 gagcgccatc ttcacgccgg taaacagcag gaaggcaccg aagatataca ggatccagtc
    10741 gaactgcgaa attagccagc tgccagtgaa gatcatgatg gtacgcagaa caatcgcccc
    10801 gagcacgcca tacaccagca cgcggcgttg taatgccgcc ggaacagaga aatagctgaa
    10861 caacatcagc cagacaaaga cgttatcgac cgccagcgat ttctcaatca gataacctgt
    10921 gagaaaggcc agtgcctgtg gatcggcaac ggcgcgacct tcggtttgca ccagatacca
    10981 ccagaaagcg gcgttaaaca gtaacgacag cgtcacccag accagcgacc aggccgcagc
    11041 ctgtttcatg gtcatggcat gtgccccacg acgcccctgc aacaacaggt cgatagccag
    11101 cataatggcg acaacaacag cgaatccgcc ccatagcaac ggcgtgccga cagtattcat
    11161 agaagttcct tacacataaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta
    11221 tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaaggaat acgtctgcgt
    11281 attcctttcg ggttgcccgg tgaccggatg tggtttttca cacatcgtaa tgacgatcga
    11341 ccggcaatga agttactccc ctttgcaggt aacaaagtat gacagaccat tcagccagtc
    11401 aatggtctgt tacatacctt ttacactgtt ttactttggc aattatagtt ttacgctatc
    11461 tgccggaaaa atcaccccag tctggcggcg gatctcggtc agcagtttgg cggtgatgcg
    11521 actgaccgcc agccccggat gatccaccag atgctcatcc accagggtag cgaacagctc
    11581 tgcttcgtag agcatggtat taatatgctg cggctgggtg agatcctgca tttggctgcc
    11641 acgcggcacg aagcacactt tctggcattc agacagtttt tcaatcacca gcgatcctgc
    11701 ttcgccctga atctcgctcg ccaggacaga atcactgact tttgagtgct gcaaggtgac
    11761 gctgaaatca ccgtaatcca tcaccaccac gccgtgggca tctacaccgc ttgccagcaa
    11821 actggcggtt gcctgcacgc ttttcggctc accaaataac gccaccgccg acgccaggca
    11881 gtaaaagccg atatccataa ttgaaccgtt agagaatgac ggattaaagg tgttgggatt
    11941 ttcaccgtcc aggtaacgtt gatagcgcga ggaatactgg caatagttga aaaagacttt
    12001 acgcaatttg ccgactttcg gcagcgcctg gcgcagcaaa tgaaagttcg gcaggcaggc
    12061 ggttttaaat gcctcaaaca gcaccacctg attttcccgc gcacaggcaa tggcggcatc
    12121 cacttccgcc agattcgacg ccagtggttt ctcgcaaatc acatgaattt tatggctaag
    12181 gaaaagttgt gtctgggaaa agtgcagggc attcgggctg gcaatataca ccgcgtcaat
    12241 ggcatcgctt tccgccatcg cttccagcga ggtaaacaga tgctcgacag aaaaatcatt
    12301 ggcgaagtgc tgagcctgct caaggctgcg ggaatatacg gcggttaact tgtatttacc
    12361 gctctcatgg gcggcctcga cgaactggcg agtgatccag ttcgtaccaa tcacagcgaa
    12421 acgtatcata acgcgtggat actccataaa gggattctgt cgtcaatgta gcacgccttt
    12481 ttcatcttcg acgtgcgcag atacgcatta ttcttttgcc ggacagccat gctcactaaa
    12541 cggcccaatc atgaccgatg gcccgagata tttcggctgg cgatggagga ttttggcgtc
    12601 aatcacggcg ctgacacgag cgagtttctc ccgcagttga gtataaaaac cgtgcttcat
    12661 attgaggtca ggcgcgttaa aaccgatagc gtcgatgctg tactgttttg ccagccagat
    12721 ggcgcgttgg ttatgaaatt cttgcgagat aatggtgata tggttttcac caaaaacctt
    12781 tttggcacgc actaccgaat ccagcgttga gaatccggca tagtcgcaga agatcacttt
    12841 cgccggtacg cctttagcga tcaaagcctt ctgcatacct gatgcttcat cgtaattttt
    12901 acgtccgtta tcgccactta ccagtagcca tttcactttt ccggcgtggt acaacgctgc
    12961 tgcggtatca atacgtcggg taaagtagcg attacccggc ctggccccca gcaataagcc
    13021 cacgttacgc tccggaactg cgttgacatc gctccaggtc agctgcttgc tggcgttgac
    13081 cataacgcga tcggcaataa aaatggtcgc tcctgctata agcaacaaca aacagccaat
    13141 cttaagagtc cggcgagaaa aacaaatacg gagaagaagg cggggctgca tggtcgttat
    13201 ccttaacgaa aggggggagg tcgattttag gcgctaaaga aagaaaagat aacgcctgac
    13261 atcattttca gattttgtcc tgcccaagcg acaactgcgt tagccacagc cgggtgtcga
    13321 actcaagctg gtggtactgc ggctccatat ggcagcacaa ctggtaaaac gctttgttat
    13381 gctctttctc cttcaggtgc gccagttcgt gcaccacgat catacgtaaa aacggttccg
    13441 gcgcgttgcg aaacacagta gcgacgcgga tctctgcttt tgctttcagc ttgccgccct
    13501 gcacacgaga aacagcggta tgtaacccga gcgcgttttt tagcacatgg atcttattgt
    13561 catacatcac tttattgatc ggcggggcat tgcgcagaaa ctgatttttt agatcctgag
    13621 tatattgcca gagggcttta tcggtggcgt agtcgtgtgt tcccggatag cgttttgcca
    13681 gcacatcgcc cagacgctgt tcgttaatca gcgttcgcac ctgggaaagt agctgctcgg
    13741 gataaccctg aagataagta agattgctca taaccgccca cgaaaaagag aaaaagggta
    13801 tactcacgca cccttttcag gggcaacgtc gaaattttat cattcaggag ggccgatgag
    13861 ccacttagac aacggtttcc gttcactgac actacaacgt tttccggcga cggatgacgt
    13921 taacccgcta caggcgtggg aagcggcgga tgaatatttg ctgcaacagt tggacgacac
    13981 agaaatccgc ggcccggtgt tgatcctgaa tgatgccttt ggtgcgttaa gctgcgcgct
    14041 ggcggaacat aagccgtaca gcattggcga ctcatacatc agtgaactgg cgacgcgcga
    14101 gaatttacgc ctcaacggga ttgatgagtc gagcgtgaag tttctcgaca gcaccgccga
    14161 ctacccgcaa cagccgggtg tggtgctgat caaagtgccg aaaacactgg cattgctgga
    14221 acagcaactg cgtgcgctgc gcaaagtggt cacgtcggat acacgtatta ttgccggtgc
    14281 caaagcccgt gacattcaca cttccacgct ggagctgttc gagaaagtgc tcggcccgac
    14341 caccactacg ctggcatgga aaaaagcgcg cctgattaat tgcactttca atgaaccgcc
    14401 gctggccgat gcgccgcaga ccgttagctg gaagctggaa ggtactgact ggactatcca
    14461 caaccatgcg aatgtcttct cccgcaccgg gctggatatt ggcgcacgct tctttatgca
    14521 acatctgcca gagaatctcg aaggtgagat tgtcgatctc ggctgtggta acggcgttat
    14581 tggcctgacg ctgcttgata aaaacccgca ggcgaaagtg gtgtttgtcg atgaatcgcc
    14641 gatggcggtc gcttccagcc gaatgaatgt tgaaaccaac atgccagagg cgttggatcg
    14701 ctgcgagttt atgatcaaca acgcgctctc cggagtggag cctttccgct ttaatgctgt
    14761 gctctgcaac ccgccgtttc atcagcaaca tgcgctgacc gataacgtcg cctgggagat
    14821 gttccaccac gcccgccgct gcctgaaaat caacggcgaa ctgtatatcg ttgccaaccg
    14881 tcacctggat tacttccata aactgaagaa gattttcggc aactgcacca ccatcgccac
    14941 gaataataaa tttgtggtgc tgaaaacagt gaagctgggg cgtcgtcggt aagattggga
    15001 gttacctgcc ggatgcggcg ttaacgcctt atccggccta cgttcagcac gttactccca
    15061 ggccggataa gacgcggcaa gcgtcgcatc cggcaatgtc tgcccagttc tggaatcgcg
    15121 cttccataaa acaaaaaatc cctgtcacca tcctctacat tctcttgttt agcgttgttc
    15181 aacgtttatg ctacatacgt gaagttatga cgcataaaga atacgatttc tttaccgctg
    15241 ttcatcgtac taaggggaaa aaatgattgc gattgctgac atcttgcaag caggagaaaa
    15301 gctaactgcg gtggcacctt ttctggcggg tattcagaac gaggaacaat acacccaggc
    15361 gctggaactg gtagatcatc tgctgctcaa cgatcctgaa aaccccttgc tggatctggt
    15421 gtgtgccaaa ataaccgcgt gggaagaatc agcgcccgaa tttgcggaat ttaatgccat
    15481 ggctcaagcc atgcctggcg gtatagccgt gattcgtacc cttatggatc aatatggttt
    15541 aaccctttcc gatctgccgg aaattggcag taaatctatg gtgtcacgcg ttttgagcgg
    15601 gaagaggaaa ttaacgctgg aacacgctaa aaaattggca acgcgattcg gcatttctcc
    15661 cgccttgttt attgattaac gcg
//
LOCUS       DAZFVO020000120        15665 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFVO020000120 DAZFVO020000000
VERSION     DAZFVO020000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.121"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..169)
                     /locus_tag="ACHKNB_RS17710"
                     /old_locus_tag="pgaptmp_003542"
     CDS             complement(<1..169)
                     /locus_tag="ACHKNB_RS17710"
                     /old_locus_tag="pgaptmp_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HGU4196990.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLP"
     gene            286..711
                     /locus_tag="ACHKNB_RS17715"
                     /old_locus_tag="pgaptmp_003543"
     CDS             286..711
                     /locus_tag="ACHKNB_RS17715"
                     /old_locus_tag="pgaptmp_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196991.1"
                     /translation="MKTKTFRFRKYKKKNVLLQMIVSRKQVILLRNIMTDSLLFCSVG
                     FVFMNMCDSKYTLISGEGGLRADCADYLFFAVYAIALLMIILWMTASVIYSIEEYTSQ
                     EKYGKSKVYSYFWFFIWFFVFNCAVYSSLKAINIKDIIS"
     gene            complement(704..1591)
                     /locus_tag="ACHKNB_RS17720"
                     /old_locus_tag="pgaptmp_003544"
     CDS             complement(704..1591)
                     /locus_tag="ACHKNB_RS17720"
                     /old_locus_tag="pgaptmp_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196992.1"
                     /translation="MSTSSFIFAILPGILMICIITSLFWVVIYARGKWTFSSGITVFR
                     YLQQYRWFHFGNISLLSLSVILTTRVYDTEYPLQVYVCIYIPPVMILTSLLTILTIKE
                     ATRTYKTYKSNRLTPVSVIAYAGLGYMFCFTTGIYELSSTRLNPVISDMLMSFLSRLS
                     IMLPVTLAEIAVIYLLLLALHFPDGVSARGFSVTRYLRHNDWFYYINTGNAILALILS
                     TWIYHSSISLPAALFTGALSIFFNTTIPASLSVIEHTPSDTDSKIWDTRKTSPATVLY
                     CSGTGYVIGFIVNPSAAIS"
     gene            complement(1785..2027)
                     /locus_tag="ACHKNB_RS17725"
                     /old_locus_tag="pgaptmp_003545"
     CDS             complement(1785..2027)
                     /locus_tag="ACHKNB_RS17725"
                     /old_locus_tag="pgaptmp_003545"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HGU4196993.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            complement(2108..3802)
                     /locus_tag="ACHKNB_RS17730"
                     /old_locus_tag="pgaptmp_003546"
     CDS             complement(2108..3802)
                     /locus_tag="ACHKNB_RS17730"
                     /old_locus_tag="pgaptmp_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HGU4196994.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTTPDNSEPQQV"
     gene            complement(3829..4449)
                     /locus_tag="ACHKNB_RS17735"
                     /old_locus_tag="pgaptmp_003547"
     CDS             complement(3829..4449)
                     /locus_tag="ACHKNB_RS17735"
                     /old_locus_tag="pgaptmp_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HGU4196995.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(4716..5381)
                     /locus_tag="ACHKNB_RS17740"
                     /old_locus_tag="pgaptmp_003548"
     CDS             complement(4716..5381)
                     /locus_tag="ACHKNB_RS17740"
                     /old_locus_tag="pgaptmp_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HGU4196996.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLES
                     ARQMSAFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALAAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(5522..6028)
                     /locus_tag="ACHKNB_RS17745"
                     /old_locus_tag="pgaptmp_003549"
     CDS             complement(5522..6028)
                     /locus_tag="ACHKNB_RS17745"
                     /old_locus_tag="pgaptmp_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HGU4196997.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASPLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            6866..7738
                     /gene="repA"
                     /locus_tag="ACHKNB_RS17750"
                     /old_locus_tag="pgaptmp_003550"
     CDS             6866..7738
                     /gene="repA"
                     /locus_tag="ACHKNB_RS17750"
                     /old_locus_tag="pgaptmp_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HGU4196998.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            8433..8624
                     /locus_tag="ACHKNB_RS17755"
                     /old_locus_tag="pgaptmp_003551"
     CDS             8433..8624
                     /locus_tag="ACHKNB_RS17755"
                     /old_locus_tag="pgaptmp_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4196999.1"
                     /translation="MAESGYGLAGLRMGKVKSINQYRLTPGLGGFTPVPYATTECRLP
                     CRSRGIRTIKPALNVFAKV"
     gene            8697..8948
                     /locus_tag="ACHKNB_RS17760"
                     /old_locus_tag="pgaptmp_003552"
     CDS             8697..8948
                     /locus_tag="ACHKNB_RS17760"
                     /old_locus_tag="pgaptmp_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4197000.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            8945..9232
                     /locus_tag="ACHKNB_RS17765"
                     /old_locus_tag="pgaptmp_003553"
     CDS             8945..9232
                     /locus_tag="ACHKNB_RS17765"
                     /old_locus_tag="pgaptmp_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4197001.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            9336..9926
                     /locus_tag="ACHKNB_RS17770"
                     /old_locus_tag="pgaptmp_003554"
     CDS             9336..9926
                     /locus_tag="ACHKNB_RS17770"
                     /old_locus_tag="pgaptmp_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HGU4197002.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGLQRQRGFLMWWRLANEMFISGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLML
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            10029..10523
                     /locus_tag="ACHKNB_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /pseudo
     CDS             10029..10523
                     /locus_tag="ACHKNB_RS17775"
                     /old_locus_tag="pgaptmp_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(10710..11897)
                     /locus_tag="ACHKNB_RS17780"
                     /old_locus_tag="pgaptmp_003556"
     CDS             complement(10710..11897)
                     /locus_tag="ACHKNB_RS17780"
                     /old_locus_tag="pgaptmp_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HGU4197003.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLLPDCPWQHIVF
                     TLPCQYWPLVFHNRWLLAEMSRIAADVILEICRQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSSHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDELAVEGNSKR
                     DWNRFLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDKFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELLLMHERLARQQ
                     VCG"
     gene            complement(11897..12262)
                     /locus_tag="ACHKNB_RS17785"
                     /old_locus_tag="pgaptmp_003557"
     CDS             complement(11897..12262)
                     /locus_tag="ACHKNB_RS17785"
                     /old_locus_tag="pgaptmp_003557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197004.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLHFDN
                     FDDAVECCSDLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTERKFATILAGISAGG
                     MYCPECGTVHWPDGVTPPV"
     gene            12470..14680
                     /gene="katP"
                     /locus_tag="ACHKNB_RS17790"
                     /old_locus_tag="pgaptmp_003558"
     CDS             12470..14680
                     /gene="katP"
                     /locus_tag="ACHKNB_RS17790"
                     /old_locus_tag="pgaptmp_003558"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HGU4197005.1"
                     /translation="MIKKTLPVLILLALSGSFSTAVAADKKETQNFYYPETLDLTPLR
                     LHSPESNPWGADFDYATRFQQLDMEALKKDIKDLLTTSQDWWPADYGHYGPFFIRMAW
                     HGAGTYRTYDGRGGASGGQQRFEPLNSWPDNVNLDKARRLLWPVKKKYGSSISWGDLM
                     VLTGNVALESMGFKTLGFAGGREDDWESDLVYWGPDNKPLADNRDKNGKLQKPLAATQ
                     MGLIYVNPEGPGGKPDPLASAKDIREAFSRMAMDDEETVALIAGGHTFGKAHGAASPE
                     KCIGAGPDGAPVEEQGLGWKNKCGTGNGKYTITSGLEGAWSTSPTQFTMQYLKNLYKY
                     EWELHKSPAGAYQWKPKKAANIVQDAHDPSVLHPLMMFTTDIALKVDPEYKKITTRFL
                     NDPKAFEQAFARAWFKLTHRDMGPAARYLGNEVPAESFIWQDPLPAADYTMIDGKDIK
                     SLKEQVMDLGIPASELIKTAWASASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLK
                     KVLASLTSLQREFNKKQSDGKKVSLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTD
                     ASQEQTDVASFSVLEPTADGFRNYYSKSRSHISPVESLIDKASQLDLTVPEMTALLGG
                     LRVMDINTNNSSLGVFTDTPGVLDNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALK
                     WKASSVDLIFSSNPELRAVAEVYASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            14724..15113
                     /locus_tag="ACHKNB_RS17795"
                     /old_locus_tag="pgaptmp_003559"
     CDS             14724..15113
                     /locus_tag="ACHKNB_RS17795"
                     /old_locus_tag="pgaptmp_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HGU4197006.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
     gene            15227..15531
                     /locus_tag="ACHKNB_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /pseudo
     CDS             15227..15531
                     /locus_tag="ACHKNB_RS17800"
                     /old_locus_tag="pgaptmp_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4214 a         3578 c         3923 g         3950 t
ORIGIN      
        1 gcggaagcag acgtttttgc gtatgggcga taatggcagc gctttcgcga atttgtccgt
       61 cgacgaacac cagagccgga ggcgtggtgt caggaacagg cgtggcatta aatgccgtac
      121 agccgctgag taatgacggc agaaggagtg taagtgattt ctttttcata gaataaatcc
      181 ggtatgtatc gggttacggt ataagacagg tggctggtaa caggtcttca gataaatata
      241 atttcatcag gatattgatg atgaacattc atgttcgggg tgattatgaa aactaaaaca
      301 ttccggttcc ggaaatataa aaagaaaaac gtcctgctgc aaatgatagt ttccagaaaa
      361 caagtgatat tgttacgcaa tattatgacg gactctttat tattttgctc ggtaggtttt
      421 gtgtttatga atatgtgtga ttcaaaatat acgttgattt ctggtgaggg tggtttacgt
      481 gctgattgtg ccgattattt attctttgcc gtatatgcaa ttgcgttgct aatgataatt
      541 ttatggatga cagcttcagt tatttattct attgaagaat atacgagtca ggaaaaatac
      601 ggtaaaagta aagtctatag ctacttctgg ttttttatct ggttttttgt ttttaactgt
      661 gctgtttatt cttctctgaa ggcaataaat ataaaagata taatcagcta atggcagcag
      721 agggattcac gataaaacca atgacgtaac ctgttcccga gcaataaagg actgttgccg
      781 gggatgtttt tcgtgtatcc catatctttg aatctgtatc agatggcgtg tgttcaatga
      841 ccgataaact ggccggaatg gtcgtgttaa aaaaaatact taacgcgcct gtaaataaag
      901 cagcgggaag agaaatgctg gaatgatata tccatgtgga gagtatcagt gccagaatag
      961 catttcctgt atttatataa taaaaccagt cgttatggcg cagataccgg gtaacggaga
     1021 accccctggc ggacacgcca tccgggaaat gcagcgccag aagcaggaga taaataactg
     1081 ctatttcagc cagtgtaaca ggcagcataa tggaaagacg tgacaggaat gacatcagca
     1141 tgtcacttat gacaggatta agtcgtgttg aagataattc gtaaatgccg gttgtaaaac
     1201 agaacatata acccagtccg gcataagcaa taacggaaac cggcgtcagt ctgtttgatt
     1261 tgtatgtttt atacgttctc gttgcttctt taatggttaa aattgtcagc agggatgtga
     1321 gaatcataac gggtggtata tatatgcaga cataaacctg caaaggatac tcagtatcgt
     1381 atacccgggt tgttaatata acggacagac tcagtaagga tatatttccg aaatgaaacc
     1441 agcgatactg ttgaagatac cggaaaacag tgatgcctga agaaaatgtc catttgccgc
     1501 gagcataaat aacaacccag aataaagaag taatgataca tatcattagt atgccgggta
     1561 atattgcaaa aataaatgat gatgtgctca ttattaaatc tccatcagca tggtgatgct
     1621 taaattttta cggttgtgag gtgcgtaatc aatatcatgt gtagttgtga ataatagcgt
     1681 cagcaacata tataaaaata atctgcattc ctgaaaaata tcgcggttgt gaaatattta
     1741 gtttttatcc cccttttatg ggggatgaaa agcaggctgt caggctataa ctgaacgcgg
     1801 tttctggatt ttggtgctaa tgtcacatct ttccattcac agctgcttcc ggggatacgc
     1861 tctttgcgtc gtattatacg ggagttctga cacacaataa ttaagccatt gtctgccgtc
     1921 tgaaatggca ttatagcgat gtttatatct tctgttgcag gaatataact gactttcttt
     1981 tcacatgtat tggtgtatat gacaactctg aatttttctg cggacatttt caatcctctt
     2041 ttaaaccaga atatcccgtg agggcattct ttgtgaaaaa gatgccggaa aaattccggc
     2101 acggggatta aacctgttgt ggttctgagt tatccggagt ggttaccggt tcgctgacat
     2161 ccggaacctt ttccggggca ggcatgacca gtggacgtgt cagggctgta agtccttcac
     2221 cggataaacc cacctctttc agcagggagt cgatgagcgg agcctgtgta cggtaagcca
     2281 gagcggcggt taccgcctgt tcagcgagat ttccgccact tatgtcggtt gttgctgttt
     2341 taccacgggt aacaccgcca ttcaggccat ccacctgaat gattttaatg ccgtcaatgg
     2401 atttcattgg ttctgtggac ttctctatga cggcaggtaa tgcatccagc agggcaagac
     2461 ggaaccgaag gctggtctgc tcatccgaaa gcacgttaat ggcatcgttc agtgcgcgct
     2521 gtgcttccgc ttctgccaga cctttctgac gggtggcctc cgccagttca ataattgctg
     2581 ctgcctggag ttcggctgct tctttttcag cctgggcgcg aaccgtcagt tcaaccgctt
     2641 tcgtttcagc ttcctgagca gcagcaatca gggcaacctg tttggtacgg tcggcctccg
     2701 cagtctggcg ggtggtttcc acatcctgct gggcctgtac ggcttcagcc agtgctttac
     2761 ttgcccgggc ttctgcctgt gactgctctt ccgattttgc cgccacggca atggctctgg
     2821 cctggctggc gatttcggtt gcctgctgtt gctcaatttc ccgtatggcc acttcccgtt
     2881 cggcttcgac ctggcgggta cgcactatct gctgtcgttc gatttcggct tcctctatct
     2941 tacgttcagc cagaattcgc gcggattctg cttcacgacg tcgttcagct tcaatagcag
     3001 caatactggc actctgctct gccgtacggg tttttacccg ttgttgttgc tccagagtca
     3061 tgaaagcttc ctgctgttca atctccagac gtcgggagag tgcatcccgg tttttttccc
     3121 tgatggccac ttcaacatcc tgctcaactt cgttacgttc gcggcgacga cgttctgttt
     3181 cctgggtcag aagggtcagg ccttcagcat caaaggcatt gtcaggatta aaatgttccc
     3241 gtgcagtctg gttgaaactt gtcagcgaca cactttcaag ctccagaccg tttttggata
     3301 agtcttccgc aacggtgttc tgcacaccct gaacaaagtt ctcgcgggca tcctgtaaat
     3361 cctgcatgct cattctggcc gccgtggccc gcagggcatc aacaaattta tcctcgacca
     3421 gactacgcag atcttcaggt gtgagcgtgc gttgtcccag cgtctgggct gcagtggata
     3481 ttccttcggc tgatggtttt acccgaagaa aaaaggcaac ggcaacatca acacgcatgc
     3541 ggtcgcgggt aatgagactt tcagccgctg cacgactgac ttccagcttc agggtgttca
     3601 tgttgacggg aattgtttcg tgaaaaatgg gcataacaat tgcaccgcca ctcattacca
     3661 ctttttggcc tcccagacct gtgcggacaa aggcctgctc tgccgatgcc cgacggtaca
     3721 ggcgggcaaa tatcagcccg ataacgaaca gggtgaggac cagcgcaata acggtaaaca
     3781 accatcccgg aagattaaac atagtgagaa cctctgtata tgtcgggatt aaagaacatc
     3841 cggccaggga ttcagttcgc ccagccagcg ggaaccggaa agacggcaga caataatcac
     3901 tttgtctcct cgtctgaatt ctttcccggc ctccggctcc agcaacaggt aatgaacctg
     3961 cccaaagcgg tctgtgaagc gaccttcaca gggggaaccc gccactgcgt gataaccggt
     4021 gatggtggcc atacacccga tgaatgcctc ctcggtgagc gccgttgttt catcacgggg
     4081 taaccagggg gcaatcagtc tgccggtgcg gtgtacgaga ggacatgtca gcagcaggca
     4141 aagtggtgca aggagaatgt tcggggcagg agcctgcagc agatgaataa taagaccctg
     4201 caggagaatg ccggatatac caaaaaaacc tgccagcata cagaggacga tcagcagggg
     4261 gatccgtccg atgttcagcc agtccagcgc ctctcctgtc agcccctccg gtaaatcagg
     4321 tgtatccata gctgaagaga gggagtgtcc gcagagcagg gcgataagtt cagcgatacc
     4381 agtaagcaga acaaaagtca gggctgccag atagggtgcg ttatacgcgg taagtaacgt
     4441 cagtgtcatt tttcctcctg tggcaggtca gtcaaacaac attatggcgt gaagcttccg
     4501 cttctttaat tgtcagaaga agcatatcgt cagtcatcag aagacaaggg gatgtctgca
     4561 ggaggggatg ataacaacag aagcctcacg ccggaatgcg ctttatgaca accggagagg
     4621 acggataata aaaaatggga catccacatc agcaaaccgc gatttgcgaa aaaaagtcag
     4681 cggagagtgg ctgccgttgt ggctgtcccg gtatttcagt gtaacaggtg attgctgtga
     4741 tctatgcccc attcggcttt cggtaaacct gaatgcccca catgaagaat gcgtgacagc
     4801 atgtttctgc cgctgccgtt caggctgcat tcgcgtccgt gcgcgagtat aaaaccttca
     4861 tcccggaata tctcttccac ttctgaagat tcgagggatg ccggatgaat taatgaaaat
     4921 gccaccggtc tgagcattct gcccgtggtg gaaaaggtgt ctctggtact gttcagctgc
     4981 aggatatgac gtttacctgt ttgcgcacgg gaaagtgtct cactcagcag ctggtcgttc
     5041 catgcgcacg tgccaatctg ccatcccaga cttctgcatg cggcaagagc cgagcggaag
     5101 cggtacaggt cgtataatgc tgcgttattg ccactttcca gcagtgattt cagcgtattt
     5161 gctaccggta tgtccgtatt ggtggccagc agttttcgca gagtcgtaaa ctcttctgcc
     5221 attcctctga atgcgctcat ctgacgcgca gattcaagcc agttcagcag gtaacgccgg
     5281 cgacgccgtt cgttgtttat gttgtccggg gcggtggaga gtgcttcgag catattctcc
     5341 agtaacagaa tgacagggtt ttcctgggat agaatggtca taaaggcgtc ctgaaaaaat
     5401 gcccgacagc cgctctccgt acaccgtaag gtgtcaccgg tgccgggcat tgtggaaaca
     5461 gaagggaaaa aaatcccggc cataaaggcc gggaaaagtc gtcaaattaa gacagcaatt
     5521 ttcatgtcaa cgctccgtca tcagatacca ggtgcaggag cagggcgatc ctcgactccg
     5581 gcagaggctg tgcatcaagc cggaggaggt actggtaacg cgcttttgtc agccagaggg
     5641 gggaggcagc atccagctcc tccctggctg ccggattgag cgcaggatcc gggtacactt
     5701 tcatcaaaga ctgaacgata tcctccctga cggttttgat ttcaccggcc attttcacga
     5761 agtccagaaa ggcactgtga cggcgaaggt gagtgaagct gtggctttcg gggtctgctt
     5821 tcaggaaaaa gtaaccggga aacacggcgt gtatatgtcg acggtaacca cagactttat
     5881 ccgtgcggcg gacctttttc agtatcaggg gtgtccaggg aaggacgtgt tgttcggaaa
     5941 gccaggaaaa gagatgttcg cggtttttgc cggcagggat gtactgtgcc agataccagt
     6001 tgcgttgatg taattcactg atattcaaaa tccggaacgc tccgcgaaaa tcgatccccg
     6061 cagggggctt cgatgttgtt gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg
     6121 ctgaccctcg ttcagcccgg cctgcagttc tgacaggcac atgatttacg cactcttgta
     6181 agccttgtgg acggttccga ggcgggcgaa aaccgacaac tcctgcaaac aggattaatg
     6241 gctggcaaat atattacata aggatgcatc tgtggcaaga gcgaagataa gcagttgaat
     6301 agatcgttat atttgataaa acagcatatt atgctgtttt gtgtgaggtg tttggatgat
     6361 tggatcgtag atcgtaatta caagatcgat tcatccaaaa aacatgaaaa acacagcata
     6421 aaagatcgtt tgcggaattg ttcatttttt tgacacagtt caaaaaaacg acgctttttc
     6481 ggggagttaa agagggcaac tgtaaccctg agaaatcatg tcggcttttc aaagcggtgg
     6541 aaacgaggta tattgcggat cgttatgcag tggcttttgg gatcctcgcg atccggaaag
     6601 tcagaaaacg gcaggatgcg ccataaggca ttcaggatgt atggcagaaa cgacggcggt
     6661 tggccggggc cggaaggctg aaaaaagttt cagaagacca taaaggaaaa cccccataat
     6721 cttcttatcg aacttggcgg aacgtagaga agattatggg ggcctcacag aatacggggt
     6781 aagtatatat gaaaccgtgt cagagattca acccttcgca gtgcattaac gcacggcatt
     6841 ctgtttccgc cataagcgac agcttgtggc ggccctgaac cagacctcat ttactgaaag
     6901 cacataccgt caggtaaaga acccgaatcc ggtcttcaca ccccgtgaag atgccgggac
     6961 gctgaagttc tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
     7021 tgccgcgcat gtggcgtatg cccgttcgcg cggcctgcgc cggcgtatgc cgccggtgct
     7081 gcgccgccgg gcgattgatg cgctgctgca ggggctgtgt ttccactacg atccgctggc
     7141 caaccgcgtc cagtgctcca tcaccacgct ggccattgag tgcggactgg cgacggagtc
     7201 tgaggccggg aaactctcca tcacccgtgc cacccgtgcc ctgaagttcc tgtctgagct
     7261 ggggctgata acctaccaga cggaatacga cccgacaatt ggctgtaaca tcccgaccga
     7321 catcacgttc acgccggcac tgtttgactc acttgatatc tcagaagagg cagtggcttc
     7381 cgcacgccgc agccgcgtgg aatgggaaaa caagctgcgc aaaaagcagg ggctggatgc
     7441 gctgggtatg gatgaactga tagcgagagc ctggcgtttt gtgcgcgagc gtttccgcag
     7501 ttatcaggca gagcttaagt cccacggcat gaaacgtgcc cgtgcgcgcc gcgatgcagg
     7561 caggacccgt caggacattg tcaccctggt gaaacggcag ctgacacgtg aaatcgcgga
     7621 agggcgtttc cgcggaagcc tggaggccgt gaagcgcgaa atcgaccgtc gcgtgaagga
     7681 acgcatgata atgtcgcgga acaacaacta cacacggctg gccactgcct caccctgagt
     7741 ttgacctcat ctgaataatc cggcctgcgc cggaggtatc cgcacgcctg aagcccgcaa
     7801 gcgcacaaaa aagcagcccc acacacaaaa aacagccacg tcatccacct tcaggtgcat
     7861 ccggttcccc ctgtttttca cgcaaaacac gtctcacaga cagggaattc ttcttgtcca
     7921 catttaactg caagggactt ctccataagg ttacaaccgt tcataccata aagtgccagc
     7981 cgtaggcgtt acagggtgca atgtttcttt taaaccccag tactgtattt cctttaaact
     8041 acttaattac ttccatttaa aaagaaaacc tattcaatgc ctgtcctgtg gacagacaga
     8101 tatgcacctc ccacgctaac gcgcgggccc cgaacctcac cgttctgaaa ccacagcaaa
     8161 aagacatcag gaataaaaac accacacaaa cgcagcaccg tgcccatccc tcataactga
     8221 aaagcgaggc cgcccccgcc cgaagggcgg gaacaacatc gcttttaatt atgaatgttg
     8281 taactaaaca tcttcattgc tgtcagtctt ctggctagat gttctcagta cacgctcgta
     8341 agcggccctg acggcccgct aacgcggaga tacgccccga cttcgggtaa accctcgtcg
     8401 ggaccactcc gaccgcgcac ataagctctg tcgtggctga aagcgggtat ggcttagcag
     8461 ggctgaggat gggtaaggtg aaatctatca atcagtaccg gcttacgccg ggcctcggcg
     8521 gttttactcc tgtgccatat gctacaacgg agtgccgcct tccatgccgc tcacgcggca
     8581 tcagaacaat aaaaccagca ttaaatgtat ttgcaaaagt atgagtaata cagacttgtt
     8641 aaccggtcct ttcagaggta tctttaagac ttgttaaaca gtctcaaagg tacgttatgc
     8701 ctaacatcat tctgtctgac accagtgcaa gtgtcagtga actgaaaaaa aatcctatgg
     8761 ctacagtcag tgccggagat ggttatcctg tcgccattct taaccggaat cagccggcat
     8821 tctactgtgt gcctgcggaa ctctatgaaa ggatgcttga tgcgctggat gaccaggaac
     8881 tggtgaaact ggttactgaa cgcagcaatc agccactgca tgatgtggac ctggacagtt
     8941 atttatgact tatacagtaa aattcaggga tgatgcgctc aaagaatggc tgaaactgga
     9001 taaatccatt caacagcagt ttgcgaaaaa actaaagaaa tgcagcgaaa atcctcatat
     9061 tccttccgca aaactgcgtg gattaaaaga ctgctacaaa atcaaacttc gcgcatcggg
     9121 gtttcgtctg gtctatcagg ttattgatga catgctaatt attgcggttg tcgcggtagg
     9181 taaacgtgaa cgtagtaacg tttataatct cgccagtgag agaatgagat agttatcttt
     9241 aaagctgact gatattactt taaaagtatt tctgatagtt atgttatttt attccacaat
     9301 acagaatatt ttctttattc tttaaggtcg tttttatgat gaaaaatcca ttagcttatt
     9361 ttcatattgc caccttttta ttgtcaggta aaagtcgtaa tctgtcagaa tactggaaag
     9421 ccgaagtcat catgcgggat aagtttgtct tacaccgcct gataagaaac gggttgcaga
     9481 gacaacgagg ctttctgatg tggtggcgcc tggccaatga gatgtttatc tctggaaaca
     9541 aaaaacaaag gaaatgtgcg ataaagataa aaaatgcact tatggaacgg tatggatgtg
     9601 atatcggcct gggcgcgcgg attggtaaag gcctcatgtt acctcatcat gcaggaatcg
     9661 tcattcatgg taatgttgat atcggtgaga atgtcattat acggcagaat acaaccattg
     9721 gtgaaaaggt ttctgacagt agggaaaact atattgttat tggcgataat gtcgatattg
     9781 gtgcccatac atgtattatt gggttaaatg tgaaaatcgg aagtaatgtt aaaatcgggg
     9841 caatgtcttt tattatggaa gatgtacctg ataactgtac ttatgtcacc aggaaggaat
     9901 ccagagtgat aattcgcgaa cagtaatgaa atacatgtta tcattcttct gttggtgact
     9961 ttgccgactc agatatatac tatctgtatg tcgtgcatta tatattaacg atacgcagag
    10021 gtgtgtatat gggcagtgtt acaaagaaac cgtcacctgt tgttattatt aagaaaaaaa
    10081 ggcgaattct ttctcatcag gatgatgccg tacaggcagt tcctgatgat ataaaaccag
    10141 cgccagtaat ggaatttaaa accgactcat tacctcctga aataccagcc acagtggccg
    10201 gttccccgga acaacagaaa aaaacaggac gtaagaagaa gaaaaaaaag aagaacaata
    10261 gatggaatca tgagtttatc cgacacagtc tggactgtgt taaaaaaaca ttccctcagt
    10321 tacgggctga agagggggga tatcatgcat ttaaaatagg catctcaaag gatgtcatgg
    10381 cttttatttc ggccagtcct gagtgtggac taactgtaaa agaatggaat tgtgctgtaa
    10441 ggatgattac tggcggttac aaatatctgc agcgtatatc agtcgcaggt actccgcgtt
    10501 atggtctgga tggcaaagtt catcgatagg aatttaaatc cccaaaagac taaaaaagca
    10561 ccacaaagcg gatgtttctt caacacccct tgtgcaccat atgagcggaa acgactatta
    10621 aacaggatga cactgattga tacagggtat gaatggcggt tttttgctcc gtttccggta
    10681 aaatggacgc aacttcccct ctgaggctct cagccgcaca cctgctgtcg tgccagtcgc
    10741 tcatgcatca ggagcagctc tgccagtcgg taaccccgct tcagccccgt aaaacgcatc
    10801 tgacttccgc acagaatgca cttcagcggg tcaaccttca gtaacctctg atacatccct
    10861 ctccacctga tttgcatcgc cgtttttctc accgtctccg ttatgacgta cacaacttct
    10921 tccagtaagc ggcgcttcac cggactcagg aaaccgtagt acctcaccat acggaacccc
    10981 ttatctgcta catgccagga gaacctttcc atgaacttat ctccactcat caacaggtat
    11041 tcttcccgtt ttgtacggtg actgttgtaa cgcagaccga tttcatcctg accggcataa
    11101 tgttccagcc gactcatcgg caccggtggc tttttcaggt aagagccgaa gtacaccgcc
    11161 acatgtgtgg cgttatccat caccctggat atgttgacat tccagccgcg gcggtaatgc
    11221 gtgtccagga agcgattcca gtcccgtttg ctgtttcctt ccactgccag ttcatccggt
    11281 attaccagct ccgggtattt ccgggacagt agccgcgtta tccggtaacg ccacatgctc
    11341 atcaccttac gggcgtaaaa atgaagattt ttccaggtgt ggctcgacgt cacaccaccg
    11401 gcagttgtcg ataaatgaat atgcggatgc cactgctggt cacgccccca tgtgtggatc
    11461 accgtgaata tccccggctc cacatctgcc tggcggcaga tttccagtat cacatccgca
    11521 gcaatgcggc tcatctctgc cagtaaccac ctgttgtgga acaccagggg ccagtactgg
    11581 cagggaagtg tgaacacaat atgctgccac gggcagtcgg ggagcagact cagcagatac
    11641 tgtatccact gtgcgccagc cttcactccg cagtgcgggc aggagcgact tttacaccgg
    11701 aagcagacct tttttgtgtg gcaacagtcc ggtgatgaac agcaccattg cgtgtagccc
    11761 atcagcgtgg tcccgcatgc catgattttt gtcactgact cagtcaccac cggacgcact
    11821 gaaccttccg gctgtttctc cagccagtta agccagcggt taccctgctg aaaaatatcg
    11881 gcaaaacggg gaagcatcag acgggtgggg taacgccatc cggccagtga actgtgccgc
    11941 attccgggca gtacatacca ccggcactga taccggcaag aatggttgca aacttccgct
    12001 ccgtacagcg ggctatttcc ggataccctt cgtcatcgac acgaacaaac cagaagacca
    12061 gctttttgtt tcctgcatcc agaaagaacg gaatacccag gtcagagcag cactcaacgg
    12121 catcgtcaaa attatcaaag tgcagaactt cagcgtcttc ttcgtcaaaa aaatcatccg
    12181 gatgaagttt cacaacatag cggggaagtt tgcttctttg tggtgcgggt ttgcgtttac
    12241 ggggtttagc tgaacgggcc atataactac ctgaaagaca atgacattat ctttttttat
    12301 aacggtaatt ccaggccatg acaagccgca gccgccaggc tgcctactcg gaacccctta
    12361 gtattttttg tctgtagtat ctatcccagc aataggtata tcctgttgca tcaataaagt
    12421 tgacttttgt atacaacatg cgaatttccc ttaatccgga gctattcgta tgataaaaaa
    12481 aactcttcct gttctgattc ttctggcgct atcggggagc ttttctaccg ctgtagccgc
    12541 tgataaaaaa gagactcaaa atttctacta tccagaaaca ctggatttaa ctcctctgag
    12601 attacacagc cctgaatcaa atccctgggg ggctgatttt gattatgcca ccagatttca
    12661 acagctggat atggaggctc tgaaaaaaga tatcaaagat ttgctgacaa cttcccagga
    12721 ttggtggcct gcggattatg gtcattatgg tcctttcttt attcgtatgg cttggcacgg
    12781 tgccggaaca tacaggacat atgatggccg gggaggcgcc agtggtggtc agcaacgttt
    12841 tgaaccgctg aacagctggc cggataacgt taatctggat aaagcccgtc gattgctgtg
    12901 gccagtcaag aaaaaatacg gctccagtat ttcctgggga gacctgatgg tcctgactgg
    12961 taatgttgcc cttgaatcca tgggatttaa aacgctggga tttgctggcg gaagagaaga
    13021 tgactgggag tcggacctgg tatactgggg gcctgacaac aagcctcttg cagataaccg
    13081 ggataaaaac gggaaacttc agaaacctct tgccgccacg cagatgggac ttatttatgt
    13141 caatcctgaa ggccccggtg gaaaaccaga tcctctggct tccgcgaaag atatcaggga
    13201 agctttttca cgtatggcca tggatgatga ggagactgtg gccctgatcg cgggagggca
    13261 tacatttggt aaagcacatg gtgcagcgtc tcctgaaaaa tgtattggcg cagggcctga
    13321 tggtgcacct gtggaggagc agggactggg atggaaaaat aaatgtggta caggaaacgg
    13381 caaatatacc atcaccagtg gcctggaagg agcctggtcg acatcgccaa cccagttcac
    13441 aatgcagtat ctgaagaatt tatataaata tgaatgggag ctgcacaaga gtcctgccgg
    13501 tgcttatcag tggaagccta aaaaagcggc aaatatagtt caggacgcgc atgatccttc
    13561 tgtcctgcat ccgttgatga tgtttacgac ggatattgct cttaaagttg atcctgaata
    13621 taagaaaata accacccgtt tcctgaatga tccaaaagct tttgagcagg cattcgcaag
    13681 agcatggttt aaactgaccc accgggatat gggaccggca gcccgatatc ttggtaatga
    13741 agttcctgca gaatcattta tctggcagga tcctcttcct gcggcggatt atacaatgat
    13801 tgatggtaaa gacattaagt cgctgaaaga gcaggttatg gatttgggta tccctgcatc
    13861 tgagctgata aagacagcct gggcttcagc ttccacattt cgtgtgactg attatcgtgg
    13921 gggaaataat ggtgcccgca tcaggttaca gcccgaaatt aactgggaag ttaatgagcc
    13981 tgaaaaactg aagaaagtac tggcatccct gacctcatta cagcgtgaat ttaacaaaaa
    14041 acagtctgac ggaaagaaag tgtcgttggc tgatttaatt gttctttcgg gtaatgctgc
    14101 aatcgaagat gcggccagaa aagccggggt ggaacttgag attcccttta ctccgggaag
    14161 aactgacgcc tctcaggagc agacggatgt tgcctcattc agtgtactgg agccgacagc
    14221 agatggattc agaaattatt actcaaaaag cagaagtcat atatcgccgg ttgaaagcct
    14281 cattgataaa gccagtcagc tggatctcac cgttcctgaa atgacggcat tactgggtgg
    14341 tctgcgggta atggatatta atacaaataa ttcttcgttg ggagtgttta ccgatacccc
    14401 tggtgttctg gataacaagt tttttgttaa tctgctggat atgtcaacac gatggagtaa
    14461 agcagataaa gaagatacat acaatggatt cgatcgtaaa acgggagcat taaaatggaa
    14521 agcatcctct gttgatttaa tcttcagttc aaatcctgaa ttacgtgcgg tggcagaagt
    14581 atatgcctcg gatgatgcga gaaataagtt tattcatgat tttgttaaat cgtggaataa
    14641 agttatgaat agcgatcggt ttgatttaaa caataaatga taaaactgat gagggagggc
    14701 acctccctca tgaaggagaa attatgcaga aaagcaataa agttataatc agctgtggta
    14761 tggcattata ttttttgtgt ggggggatta cagtggcaga agaacttgat gaaagcatgc
    14821 aagttcttgc aaggaatatg agtgtacttc agtcatcaat ggataaaaag gagcttcttg
    14881 aagctcttga cagaatggat gatgctgtag atgagtcaat gaagcgtctt cctgagagca
    14941 tttcaccggc tgatgaagaa gggaagaatg actatataaa agaacttcaa aagcttgaaa
    15001 aagagattat gctggcgaaa caaaaagtct tatcgggaca gttatcagat atacccgatt
    15061 cggtaaacaa gatgaataaa atcagagttg aggggcatga taaattcaga taaatcagtt
    15121 ggttgattcc tgcgggacga atacatacgt caggaacata actttatggc agtgaaccgc
    15181 cccgggaatc ctggagacta aacttcctga gaaagaggta aacaggatga ctaaaaatac
    15241 tcgtttttcc cccgaagtct gtcaacgggc gattcatatg gttctggaaa gtcagggcga
    15301 atatgactca caatgggcgg caatttgttc cattgcccaa agattggctg tacaccggag
    15361 actctgcgtg tctggggacg ccagcatgag cgggataccg ggagcggtga tggtgggctc
    15421 accagcgctg aacgtcagcg tctgaaagag ctggaacgtg aaaaccgtga actgcgccgc
    15481 agtaacgata tccttcgcca ggcttccgct tattttgcga aggccgagtt catcgatagg
    15541 aatttaaaac ccaaaaagat taaaaaaaca cccaaaaacg gatgtttatt caacactgcc
    15601 tgcgtgccat gagtgcaccg ccgtcaatta aatggatctt atcgattgat actgcatatc
    15661 actga
//
LOCUS       DAZFVO020000121        15634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFVO020000121 DAZFVO020000000
VERSION     DAZFVO020000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.122"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..208)
                     /locus_tag="ACHKNB_RS17805"
                     /old_locus_tag="pgaptmp_003561"
     CDS             complement(<1..208)
                     /locus_tag="ACHKNB_RS17805"
                     /old_locus_tag="pgaptmp_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HGU4197007.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLK"
     gene            complement(386..1297)
                     /gene="gsiD"
                     /locus_tag="ACHKNB_RS17810"
                     /old_locus_tag="pgaptmp_003562"
     CDS             complement(386..1297)
                     /gene="gsiD"
                     /locus_tag="ACHKNB_RS17810"
                     /old_locus_tag="pgaptmp_003562"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HGU4197008.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1300..2220)
                     /gene="gsiC"
                     /locus_tag="ACHKNB_RS17815"
                     /old_locus_tag="pgaptmp_003563"
     CDS             complement(1300..2220)
                     /gene="gsiC"
                     /locus_tag="ACHKNB_RS17815"
                     /old_locus_tag="pgaptmp_003563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HGU4197009.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2238..3776)
                     /gene="gsiB"
                     /locus_tag="ACHKNB_RS17820"
                     /old_locus_tag="pgaptmp_003564"
     CDS             complement(2238..3776)
                     /gene="gsiB"
                     /locus_tag="ACHKNB_RS17820"
                     /old_locus_tag="pgaptmp_003564"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HGU4197010.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEKATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3796..5667)
                     /gene="gsiA"
                     /locus_tag="ACHKNB_RS17825"
                     /old_locus_tag="pgaptmp_003565"
     CDS             complement(3796..5667)
                     /gene="gsiA"
                     /locus_tag="ACHKNB_RS17825"
                     /old_locus_tag="pgaptmp_003565"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HGU4197011.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5654..6619)
                     /gene="iaaA"
                     /locus_tag="ACHKNB_RS17830"
                     /old_locus_tag="pgaptmp_003566"
     CDS             complement(5654..6619)
                     /gene="iaaA"
                     /locus_tag="ACHKNB_RS17830"
                     /old_locus_tag="pgaptmp_003566"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HGU4197012.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6823..8058
                     /gene="moeA"
                     /locus_tag="ACHKNB_RS17835"
                     /old_locus_tag="pgaptmp_003567"
     CDS             6823..8058
                     /gene="moeA"
                     /locus_tag="ACHKNB_RS17835"
                     /old_locus_tag="pgaptmp_003567"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HGU4197013.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLATLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8058..8807
                     /gene="moeB"
                     /locus_tag="ACHKNB_RS17840"
                     /old_locus_tag="pgaptmp_003568"
     CDS             8058..8807
                     /gene="moeB"
                     /locus_tag="ACHKNB_RS17840"
                     /old_locus_tag="pgaptmp_003568"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HGU4197014.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8986..9648)
                     /gene="fsa"
                     /locus_tag="ACHKNB_RS17845"
                     /old_locus_tag="pgaptmp_003569"
     CDS             complement(8986..9648)
                     /gene="fsa"
                     /locus_tag="ACHKNB_RS17845"
                     /old_locus_tag="pgaptmp_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGU4197015.1"
                     /translation="MELYLDTSYVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9779..10678
                     /gene="ybiY"
                     /locus_tag="ACHKNB_RS17850"
                     /old_locus_tag="pgaptmp_003570"
     CDS             9779..10678
                     /gene="ybiY"
                     /locus_tag="ACHKNB_RS17850"
                     /old_locus_tag="pgaptmp_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGU4197016.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10684..13116
                     /gene="ybiW"
                     /locus_tag="ACHKNB_RS17855"
                     /old_locus_tag="pgaptmp_003571"
     CDS             10684..13116
                     /gene="ybiW"
                     /locus_tag="ACHKNB_RS17855"
                     /old_locus_tag="pgaptmp_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGU4197017.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALTHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13262..14077
                     /gene="ybiV"
                     /locus_tag="ACHKNB_RS17860"
                     /old_locus_tag="pgaptmp_003572"
     CDS             13262..14077
                     /gene="ybiV"
                     /locus_tag="ACHKNB_RS17860"
                     /old_locus_tag="pgaptmp_003572"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HGU4197018.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14229..15494
                     /gene="ybiU"
                     /locus_tag="ACHKNB_RS17865"
                     /old_locus_tag="pgaptmp_003573"
     CDS             14229..15494
                     /gene="ybiU"
                     /locus_tag="ACHKNB_RS17865"
                     /old_locus_tag="pgaptmp_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HGU4197019.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGHFTLADLNIHGKRALGMDV"
BASE COUNT         3799 a         4306 c         3971 g         3558 t
ORIGIN      
        1 cctttaatgt cagggccgaa cgttcaatat ctttaaagaa atagccaacg tttttctgaa
       61 taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc cgataccagt tgatagttaa
      121 tgatgaaaaa aagaccaata aatgacagtg ctgccaataa aaataaaatt atcagcctta
      181 tctttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa ctatctgaac
      241 gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt aaccaaatta cccgattcta
      301 caaaatcagc agaaagttac aacacttgct tagggcaata attgcctgtg aggtgattac
      361 cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc gcatcgcgta
      421 aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg gccggaaaaa
      481 cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt gctccccact
      541 ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca gagataatcg
      601 aggtgccaat gcgcatggtg aaaaacacca cgatagacga gacggtccca ggcaagatat
      661 gacgcaataa aatggtcata tcgctggcac caatactgcg tgctgactca ataaaggttt
      721 gctgtttcaa caccagcgtg ttgccgcgca ccaggcgggc aaacgcgggg atggaaaaaa
      781 tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg
      841 ccagtaaaat acccgggaag gcaaacagca catcgcaaat gcgcatgatc agccgatccc
      901 accagccttc ataatatcca gccagcaagc ccagcaacgt cccgatcgcc gcaccgataa
      961 acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa
     1021 tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa agaaggtccg ttattcagat
     1081 tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca aaaatggcca
     1141 ccacaatcaa taaaataacg aataatgcgg cggtcatcgc catatgctga cggcgaaatc
     1201 gtcgccagaa ttcatgccac ggtgtacgca cctggtcagg tttgaccagt ggcatggcgt
     1261 ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc tgatagccgg
     1321 gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt ccagcgagaa
     1381 aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tctacggagt caacgagtaa
     1441 gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa
     1501 gccaaactgt aagcccatca tggtcactac cgggatcatc gcgttacgta gcccgtgttt
     1561 gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat aatcttcgct
     1621 taaaacatcg acaaacgacg cgcgggtaaa gcgcgccatc acggctgcca ctgccgcgcc
     1681 gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacgg taggcagcca
     1741 gcccagttca acggagaata cctgaattaa aagcatcccc agcgcaaatg ccggaaacga
     1801 gatccccgac accgcaatgg tcatactcaa tcgatccggc caacgattac gccagacggc
     1861 ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg ttatggtcag
     1921 ccacagcgtt ggcataaagc ggctggcaat ctcatcggca accggacgac gcgacaccat
     1981 cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact ggtgatacag
     2041 cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat cagcttcggg
     2101 cccggcaatc aatcgcgccg gatcgccggg cagcatatgc acaaataaaa acaccagcac
     2161 cgagacgata aacagcgtcg gaatcaaccc cagtaatcgt ttgataacgt aattaagcat
     2221 tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc ggtgtctggc
     2281 atgatccaaa aaccggtcag gtttttactg tgtgccgaca ccagtttttc taccaccagc
     2341 gggatccacg gcgactcttg ccagatgata tcctgcgccg ccttatacaa gcgggtcttt
     2401 tccgccggat cattagtttt cagtgcctga gccaggaagt cgtccacctg tttattgctg
     2461 taaaatgcgg tattaaacag cgtcggtggc cagttttgcg aggcgaacag tggcgatagc
     2521 gcccagtccg cttcgccggt tgaagccgac cagccagtgt agaacatccg cacgccgctc
     2581 tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat cgcggtcacc
     2641 tgggctttaa tcccgacctg cgctaactgc tgctgggtaa attgcagcac tttctgcgcg
     2701 gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata tcccgcctct
     2761 ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact ttgcgcgtag
     2821 gcgatacttg gcggtaccac accagtagct ggcgttgcat agcccgcaaa ggcaactttc
     2881 accagcgccg gacggttaat ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac
     2941 ggcttttgcg tcacgttcat actgatataa cgctgcataa ttgacggact ggccatcaac
     3001 tcgatatttt tgtttttctc cagcagtgtg gccttctcgt agggaatggg gaaagcaaac
     3061 tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt tatccgccac cggacgccag
     3121 gttatgctgt ccagtttggg caatcctggc tgccagtaac ccgcgaattt tttcaccttc
     3181 acaaaatcgg tctgattcca ggtatccagt tcatacggtc cggttcccac cggatgaaaa
     3241 ccaatctcct tgccatattt ttccagcgct gccggtgaaa tcatcgcggt cgccggatgg
     3301 gcaagaatat taataaacgc tgagaacggc tgtttgagag taatctttac tgttgttgga
     3361 tcgatcgcct ccgttttggc aatattctta tacaggttat agcgtttaag atgattcgcc
     3421 ggatcgctgg cccggtccag attcgctttt accgccgctg cgttgaaatc ggtgccatcc
     3481 tggaatttaa ttccttcccg caatttcacg gtgtatgtaa ggccgtcatc ggaaacggta
     3541 taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccaaa cagcccctgg
     3601 taaaacgatt tcgctacggc ctgagataac gtgtcatttg cgtcatacgg atcgagcgtg
     3661 gtgaagttcg atcctaccgc caccaccaca tctttggcag cgaatgcaca agatgccatc
     3721 aacgctgtcg caatgcccaa cgccactaac ccactacggt gtacagctct tgccatttta
     3781 ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta
     3841 atgccccggc ccgacgcatt gcaacgagac ggctgccacc tcttcgccac gcagatgaat
     3901 attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg acggttcagc
     3961 gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc
     4021 gcgccgtgga ccaatttcaa caatttgtcc gagatacatc accgccacac gatgactaat
     4081 ccgctctacc acggccatat cgtgggagat aaacagatac gcaatgccga aatcacgctg
     4141 gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg ccgagacggc
     4201 ttcgtcggca atgatcactt tcggattcaa tgccaacgcg cgagcaatgc agatgcgctg
     4261 gcgctgaccg ccggaaaact catgcgggta acgccaggca tgttcaggta acaggcccac
     4321 gcgctccagc aaccacgcaa cgcgtgcagc cgcttcttta cctggcaata aaccgtgtac
     4381 acgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg
     4441 gtcctgaaaa ataaactgaa tatcccggcg caatgcctga agtttgccgg gtgacaaggt
     4501 atcgattctc tgaccgttaa agataatttc gccgccctgc gattcgacca ggcgcagcaa
     4561 cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata gcgtttcgcc
     4621 aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa
     4681 caaaccgctg cgcaaaggga aacgggtgac aagattacgc actcgtaaaa caggttcgcc
     4741 atccaccacc gttttctgct cgatgggggg ggcctgtttc gctggatgtt caagcgatat
     4801 caacgggaaa cgtcggggat aatctaaccc tttcatcgca ccaagttgcg gaacagcagc
     4861 taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt
     4921 ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg ccaccacgcc
     4981 catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata ctttgattaa
     5041 ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat cggcaatcag
     5101 caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca tcccgccaga
     5161 gagttgatgc ggataacgtg aaagaatggt ttgtgcctca ggaatgcgta cctgatccag
     5221 catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca gacgaattga
     5281 ttcggcaatc tgttcaccca cagtaaatac cgggttcagc gatgtcatcg gctcctgaaa
     5341 tatcatcgcc atatccgcac cgcgaacatg gcgcatttgt gcagcgctct gctcgctaag
     5401 ttcaatcact tcgcgactgc gccgctgcaa cagcatttta tcgcactgta ctaaaccgcc
     5461 cgcctgttcc aacaggcgca tcaatgccaa cgcagtcact gacttaccgg agccggattc
     5521 gccaacaatt gccagcgtct caccgcgttg cagactaaaa gagagattgc ggaccgcagc
     5581 tattttctgc tggtcctgca taaaggcaat attcagattt tcaaccgcca gcacattacc
     5641 ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag ataccggtgg
     5701 ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta aacggtagcg
     5761 cgacattccc ttcatggtcg atagcgatta agccaccgct accgccaagc gcagggagtt
     5821 tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa tccattaacg
     5881 cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcccgtgccg gtacaagaaa
     5941 ccgccacact ggcgttattg gcgtagcatc cggcacccac taagggacta tcgccaactc
     6001 gtccgggtaa tttattggtc attccgcctg tggacgtggc tgccgccaaa ttgccgtcta
     6061 aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt ggcgcaccgc
     6121 tatggtcgag gacggttgcc ccttccttgc gcgctgccag tagttgttca taacgcaaag
     6181 gcgtggagaa aatctccggc gagacgcgct ccatgccacg agcaaacgca aaattttctg
     6241 ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg gcggcaagaa
     6301 ccggattacg cagatgacta acgcccgcca ccgcaccggc tttcagggtg ttaccatcca
     6361 tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca attccggcgt
     6421 taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc agcgcacttt
     6481 cgcccgcttc cagcattttc tgcccggttt caacaatggc agacaacgcc tcaatgtagc
     6541 gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgcca ccatgaattg
     6601 caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact tttgctataa
     6661 atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat ggatatggca
     6721 tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctgtggc gtttttctga
     6781 cataataggc aaattcgatt ttgcctccga aggagtgttt tcatggaatt taccaccgga
     6841 ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgtcacccc actgaccgcc
     6901 caggaaacgc tgccactggt acagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg
     6961 ccgcttgatg ttccggggtt tgataactcc gcaatggacg gctacgcggt gcgtttagcc
     7021 gatattgcct ccaggcaacc gctgcccgtt gccggtaaat cctttgccgg tcagccgtac
     7081 catggtgaat ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc ggtgccggaa
     7141 ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt
     7201 actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt
     7261 gcggttgttt tcccggcggg aactcgcctg actaccgctg aactgccagt gattgcttca
     7321 ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt
     7381 gatgaactcc agttgcccgg tcagccactg ggcgatggcc aaatctacga taccaaccgt
     7441 ctcgccgtac acctgatgtt agaacagttg ggatgcgagg taatcaactt agggattatc
     7501 cgcgacgatc cacatgccct gcgcgccgca tttattgaag ccgacagcca ggcggatgtg
     7561 gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac cattcttgaa
     7621 gagctggggg agatcgcctt ctggaagctg gcgattaaac cgggtaaacc gttcgcgttc
     7681 ggtaaacttt ctaatagctg gttctgcggc ctgccgggca acccggtttc agcgacgctg
     7741 accttctatc aactggtaca gcctttgctg gcaacactaa gcggcaacac cgccagcggc
     7801 ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt
     7861 gatttccagc gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt gacgaccacc
     7921 ggacatcagg gttcacatat atttagctcc tttagtctcg gcaactgctt tatcgtgctg
     7981 gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg aagtagaacc gtttaacgcg
     8041 ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc tacaaccggc
     8101 aaatcattct gcgcggtttt gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc
     8161 tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca gtatctggca agcgccggtg
     8221 tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctcgaatctg caacgccaga
     8281 cactgcacag tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt tacgccctga
     8341 cgcggatcaa cccacatatc gcgattacgc cagtcaatgc actgctagat gacgcagaac
     8401 ttgcagcatt gattgctgaa cacgatctgg tgctcgactg tacggataac gttgcggtac
     8461 gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa
     8521 ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg tgctatcgct
     8581 gccttagccg tttgtttggc gagaacgcat taacctgcgt agaagcaggc gtgatggcac
     8641 cgttgatcgg cgtaattggt tcgttgcagg caatggaagc gatcaaactg ctggcaggtt
     8701 atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc gatgacctgt cagtttcgcg
     8761 aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg ccacctccag
     8821 gcctgataag acgcgacaag cgtcgcatca ggcatttgct ccagacgcca gatgcggcgt
     8881 gaacgcctta tctggcctac ggttcgggca cagatttcca agcgcggcag gcatcggtgc
     8941 actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa tcgacgttct
     9001 gccaaacgct cccagccagt cctgttcaaa cttcgccaca gcggcttcca ccgccggata
     9061 gctaatcatc tgttgtgcca catccagtgg cagcgtaatt gattcacatc ctgccagtaa
     9121 gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg cctgcggcgc
     9181 atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac cgccctgagc
     9241 atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg acagcagccc
     9301 ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt ttaacatctt
     9361 aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg caataataga
     9421 acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta cctgggcaaa
     9481 cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat ccagcggttt
     9541 tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa aaatacgtga
     9601 cagcgccttc accgcaacaa cgtatgaagt atccagatac agttccataa ccatcctcaa
     9661 aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca gttgacgcac
     9721 atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag aggcaactat
     9781 gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca cggtcgtatt
     9841 tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac
     9901 gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc
     9961 tgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg aaaagttaac
    10021 cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa ccgtgtgtgg
    10081 tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac cgttttacga
    10141 tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc cggaaatggc
    10201 gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag aaacctgtct
    10261 gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt ttcttgccga
    10321 tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg ccgccagagt
    10381 gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct
    10441 gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt ttgccgccga
    10501 cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca tcaacaaata
    10561 tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct
    10621 cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac gaggataaca
    10681 accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg
    10741 ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta taccgagatg
    10801 tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact gacgcatcac
    10861 ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa ccaggcaagc
    10921 gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga aaaagagatt
    10981 gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa caaacgcgtt
    11041 ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg ctacggcatg
    11101 tttaccgatg agcaaaaagg tctgctggcg accggaatca ttaaagcgga aggcaatatg
    11161 acctccggcg atgcgcacct ggcggtgaat ttcccgctgc tgctggaaaa aggtcttgat
    11221 ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt gctggaagat
    11281 ttacatggcg aacaattcct gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac
    11341 attgaacgtt tcgctgccct ggcgcgtgaa atggctgcta ccgaaactcg cgaaagccgt
    11401 cgcgatgaac tgctgacgat ggcagaaaac tgcgatctta tcgcccacca gccgccgcag
    11461 actttctggc aggcgctgca actgtgttac ttcatccaat tgattttgca gatcgaatct
    11521 aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta ctatcgccgc
    11581 gacgttgaac tcaaccagac gctggatcgc gaacacgcca tcgagatgct gcatagctgc
    11641 tggctgaaac tgctggaagt gaacaagatc cgctccggct cacactcaaa agcctctgcg
    11701 ggaagtccgc tgtatcagaa cgttaccatt ggcggacaaa atctggttga tggtcaacca
    11761 atggatgcag tgaatcccct ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc
    11821 actcagccta acctcagcgt gcgttaccat gcaggaatga gtaatgattt cctcgacgcc
    11881 tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg
    11941 atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta cgcagcgatt
    12001 ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg catgagcttt
    12061 atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc
    12121 aaagtgttcc tgccacaaga aaaagcgttg tcggcaggca actttaacaa tttcgatgaa
    12181 gtaatggacg cgtgggatac gcaaatccgt tattacacgc gtaaatcaat cgaaatcgaa
    12241 tatgtcgtcg acaccatgct ggaagagaac gtacacgata ttctctgctc ggcgctggtg
    12301 gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt
    12361 tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggcagt gaagaaactg
    12421 gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc ggatgacttc
    12481 gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc gaagtacggc
    12541 aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta tatcgacgaa
    12601 ctgaaacagt accataaccc gcgctacggt cgtggtccgg ttggcggcaa ctattacgcg
    12661 ggtacctcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc aacaccggac
    12721 gggcgtaaag cccacacccc gctggcagaa ggcgcaagcc cggcttccgg tactgatcat
    12781 cttggcccta ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc gattctcggc
    12841 ggcgtgttgc tcaaccagaa actgaatcca gcaacactgg agaacgaatc tgacaagcag
    12901 aaactgatga tcctgctgcg caccttcttt gaagtgcata aaggctggca tattcagtac
    12961 aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca gtaccgcgat
    13021 ctggtagtgc gtgtcgcggg ctattccgcc ttcttcaccg cgctctctcc agacgcacag
    13081 gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ctcggcccgg cggcgtcgtc
    13141 gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa atataatttg
    13201 tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca ggagcacacc
    13261 tatgagcgta aaagttatcg tcacagacat ggacggtact tttcttaacg acgccaaaac
    13321 gtacaaccaa ccacatttta tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt
    13381 cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg agctaaagga
    13441 tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca agcaactgtt
    13501 ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc taaaagataa
    13561 gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa atgcccccga
    13621 agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaagctgtaa aagattatca
    13681 ggagattgac gacgtactgt tcaagttttc gctcaacctg ccagatgaac aaatcccgtt
    13741 agtgatcgac aaactgcaca tagcgctcga tggcattatg aaacccgtca ccagtggttt
    13801 tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt cgcggttact
    13861 gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc
    13921 ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg aaaacattaa
    13981 acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga atgtgattca
    14041 ggcggtgctg gataacacat ccccttttaa cagctgacct ctctaccgga gccgtgcgct
    14101 ccggttttcc cttcttttta catcctgtac attacatcaa atcattaaac ttgcattaac
    14161 ttatttataa cttatattgt cacatgcaaa tcagaatggt ttcgaatgaa agttaatgag
    14221 aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac aaagcagcta
    14281 tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga cgtccagcag atctttaatc
    14341 agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg
    14401 atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt actgcagagc
    14461 agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc ccccgcgaac
    14521 aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc tttgacgagg
    14581 tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt cccgagattt
    14641 accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg
    14701 cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc
    14761 cggatgtgag cgtcatctac cctgaccgta tccgccgccg tccgcccgga acgacctcca
    14821 aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt ccagcgtatc
    14881 agcgcgtttt cgccaacgtc tttaatggca atctggcgca atatgatccc tggcatgcgg
    14941 cacatcgtac ggaagttgaa gagtacacgg tggacaacac caccaaatgt tccgtgtttc
    15001 ggacattcca gggctggaca gcgctctctg atatgctgcc tggtcagggg ttgctgcacg
    15061 ttgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt gatgatgtgc
    15121 cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca gagcaatggc
    15181 atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga gactccgtct
    15241 ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag ggttggggca
    15301 acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg
    15361 tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa
    15421 caaactggga aggacacttt acgcttgccg acctcaacat tcacggtaag cgagcgttgg
    15481 gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa atcgattcga
    15541 cgttactcag attgctgaca agtgcgcttt cttaatgcct gatgcgctac gcttatctgg
    15601 cctacaaaag catgcaaatt caatatattg caga
//
LOCUS       DAZFVO020000122        15389 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFVO020000122 DAZFVO020000000
VERSION     DAZFVO020000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.123"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1072)
                     /gene="nemA"
                     /locus_tag="ACHKNB_RS17870"
                     /old_locus_tag="pgaptmp_003574"
     CDS             complement(<1..1072)
                     /gene="nemA"
                     /locus_tag="ACHKNB_RS17870"
                     /old_locus_tag="pgaptmp_003574"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HGU4197020.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAE"
     gene            complement(1109..1708)
                     /gene="nemR"
                     /locus_tag="ACHKNB_RS17875"
                     /old_locus_tag="pgaptmp_003575"
     CDS             complement(1109..1708)
                     /gene="nemR"
                     /locus_tag="ACHKNB_RS17875"
                     /old_locus_tag="pgaptmp_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HGU4197021.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            1811..2050
                     /locus_tag="ACHKNB_RS17880"
                     /old_locus_tag="pgaptmp_003576"
     CDS             1811..2050
                     /locus_tag="ACHKNB_RS17880"
                     /old_locus_tag="pgaptmp_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HGU4197022.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            2099..2995
                     /locus_tag="ACHKNB_RS17885"
                     /old_locus_tag="pgaptmp_003577"
     CDS             2099..2995
                     /locus_tag="ACHKNB_RS17885"
                     /old_locus_tag="pgaptmp_003577"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGU4197023.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            3076..3597
                     /gene="sodC"
                     /locus_tag="ACHKNB_RS17890"
                     /old_locus_tag="pgaptmp_003578"
     CDS             3076..3597
                     /gene="sodC"
                     /locus_tag="ACHKNB_RS17890"
                     /old_locus_tag="pgaptmp_003578"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HGU4197024.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3598..5610)
                     /locus_tag="ACHKNB_RS17895"
                     /old_locus_tag="pgaptmp_003579"
     CDS             complement(3598..5610)
                     /locus_tag="ACHKNB_RS17895"
                     /old_locus_tag="pgaptmp_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HGU4197025.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNTEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(5610..6467)
                     /locus_tag="ACHKNB_RS17900"
                     /old_locus_tag="pgaptmp_003580"
     CDS             complement(5610..6467)
                     /locus_tag="ACHKNB_RS17900"
                     /old_locus_tag="pgaptmp_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGU4197026.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(6470..6706)
                     /locus_tag="ACHKNB_RS17905"
                     /old_locus_tag="pgaptmp_003581"
     CDS             complement(6470..6706)
                     /locus_tag="ACHKNB_RS17905"
                     /old_locus_tag="pgaptmp_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HGU4197027.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6907..7341
                     /gene="slyA"
                     /locus_tag="ACHKNB_RS17910"
                     /old_locus_tag="pgaptmp_003582"
     CDS             6907..7341
                     /gene="slyA"
                     /locus_tag="ACHKNB_RS17910"
                     /old_locus_tag="pgaptmp_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HGU4197028.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(7388..7855)
                     /gene="slyB"
                     /locus_tag="ACHKNB_RS17915"
                     /old_locus_tag="pgaptmp_003583"
     CDS             complement(7388..7855)
                     /gene="slyB"
                     /locus_tag="ACHKNB_RS17915"
                     /old_locus_tag="pgaptmp_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HGU4197029.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            8129..9238
                     /gene="anmK"
                     /locus_tag="ACHKNB_RS17920"
                     /old_locus_tag="pgaptmp_003584"
     CDS             8129..9238
                     /gene="anmK"
                     /locus_tag="ACHKNB_RS17920"
                     /old_locus_tag="pgaptmp_003584"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HGU4197030.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            9336..9665
                     /gene="mliC"
                     /locus_tag="ACHKNB_RS17925"
                     /old_locus_tag="pgaptmp_003585"
     CDS             9336..9665
                     /gene="mliC"
                     /locus_tag="ACHKNB_RS17925"
                     /old_locus_tag="pgaptmp_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HGU4197031.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            9724..10380
                     /gene="pdxH"
                     /locus_tag="ACHKNB_RS17930"
                     /old_locus_tag="pgaptmp_003586"
     CDS             9724..10380
                     /gene="pdxH"
                     /locus_tag="ACHKNB_RS17930"
                     /old_locus_tag="pgaptmp_003586"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HGU4197032.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            10509..11783
                     /gene="tyrS"
                     /locus_tag="ACHKNB_RS17935"
                     /old_locus_tag="pgaptmp_003587"
     CDS             10509..11783
                     /gene="tyrS"
                     /locus_tag="ACHKNB_RS17935"
                     /old_locus_tag="pgaptmp_003587"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGU4197033.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            11842..12705
                     /gene="pdxY"
                     /locus_tag="ACHKNB_RS17940"
                     /old_locus_tag="pgaptmp_003588"
     CDS             11842..12705
                     /gene="pdxY"
                     /locus_tag="ACHKNB_RS17940"
                     /old_locus_tag="pgaptmp_003588"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HGU4197034.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(12749..13354)
                     /gene="gstA"
                     /locus_tag="ACHKNB_RS17945"
                     /old_locus_tag="pgaptmp_003589"
     CDS             complement(12749..13354)
                     /gene="gstA"
                     /locus_tag="ACHKNB_RS17945"
                     /old_locus_tag="pgaptmp_003589"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HGU4197035.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(13460..14962)
                     /gene="dtpA"
                     /locus_tag="ACHKNB_RS17950"
                     /old_locus_tag="pgaptmp_003590"
     CDS             complement(13460..14962)
                     /gene="dtpA"
                     /locus_tag="ACHKNB_RS17950"
                     /old_locus_tag="pgaptmp_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HGU4197036.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNITDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         3953 a         3829 c         3965 g         3642 t
ORIGIN      
        1 cttccgcgcc gccaccgtag aaactttcgg cacgctgtgg gttaagctca gctttgcgct
       61 gcaagcgggc gaccagatcc gggttcgcaa tccagtcacg accaaatgcc accgcatcaa
      121 ttaacccttt gccgatcagc gtttcagctt tttctactgt gtatgcacct gcgccgataa
      181 tcggaccgtg gaaacgggcg cgtacttttt cgcggaacgc atcagtatac ggttcacccc
      241 ccgcccaatc tggttctgac atatgcagat aagcaatgcc gcgtttaccc agttgttcaa
      301 tcagatacag tgcatcggct tcttcattcg ggccgttatc cgtgttctgg aaagtaccga
      361 ttggcgaaac gcgaatgcca atgcgatcgg caccccattc ttcaatcccg gcatcgacca
      421 cttccagtac cagacgtgcg cgattttcca cgctgccgcc gtactgatcg gtacgatggt
      481 ttgaagaagg agaaaggaac tgatgcagca aataaccgtg agcagagtgg agctctacca
      541 gatcaaaacc ggcttcacgc gcgttagcaa tggcctgacg gaaatcattg acgatacctg
      601 gaatctcttc cagttcaagc gcacgcggca tggatgtttc aacacggatc gcctgaccat
      661 tttcatcgcg cagagaagta cgtgttcccg cgctaagtgc tgaaggcgct accggtgcct
      721 gaccgccagg ttgcaggctg gcgtgagaaa tgcgtccggt gtgccacagc tgcacggcca
      781 tatgaccatt ttcagcatga acgccagcgg tgattttttt ccatgcggca atttgctccg
      841 gactatggat gccaggcgca cctgcatatc cttttgcctg ggcagaaatt tgcgtggctt
      901 cactaataat caaaccggca ctggcacgtt ggcgatagta ttccgccatc aacggggtag
      961 gaatgtcacc cggttcaata ctgcgcagac gcgtcagcgg tgccataaaa atacggtttg
     1021 ccgccgtgat cgcgcccact ttcagtgggg aatacagttt ttcagatgac ataaaggctc
     1081 ctggtcgtct ggtgataaaa aatgcctgct aaacggcagg cgtcgcaata atgtttttta
     1141 catgggccag cgcgttttcc agtggctcga aactgcgcga aattttggcc tgcagattcg
     1201 cgccaagcca cagtgcgtaa agcacttgtg cctgttgtag cggttcgcca caaaaggtta
     1261 aacaatggtt ctcacggcca ttttccagcg cctgagagag cagggcgatc acgccgcgag
     1321 cacctttatc catcgcgctg cgcatatctt ctgacagatc gcacacttcg gcagagagtt
     1381 ttactgtcag gcaaccactg atggttccat gttggcaaaa ctggttcagg gtttgctggt
     1441 aataagccag tatgcggtcg cggtagttac cttcgccgga ttgcagcaac tcagtcagtc
     1501 gctggtgata tgcggcgtaa tgacgctcaa gcatggcaac gccaaacgct tctttagagc
     1561 gaaagtagtg atagaaggac cctttcggca cttcagcggt ttttagtaat tcgcttagcc
     1621 ccatcccggt gaatccacgt tgcaggcaaa gttgctcgcc cgtcgccagg agatgttcgc
     1681 gagtatcatg ttcggtgtgt ttgttcatgc gttggagtgt agtagaccag tcggtctagt
     1741 gcaagcaaag ttgctacttt ggacattaac agcttcaata atggtaataa tcttatctga
     1801 ggagggcgaa gtggcggagc aattagagtt ctttcccgtc cagagcccgt gccggggaat
     1861 ttgccagtct gacgaacgcg ggttttgtcg cggctgtttt cgtagtcgtg atgagcgttt
     1921 taactggaat aaaatgagcg atggcgaaaa acaagaggtg ctgcgccttt gtcggcagcg
     1981 attaatgcgc aaattacgcg caaataagcc agcgtcatca gacgaaccga agcaaccatc
     2041 actcttttga acctaatctt gcgtatactc ctctcatatc tgataagagg aagcgattat
     2101 ggttcagcgt attactattg cgccgcaagg cccggagttt tcccgttttg tgatgggcta
     2161 ctggcgattg atggactgga atatgtccgc ccgccagcta gtcagtttta ttgaagagca
     2221 tctggatctc ggcgtgacca ccgtggacca tgctgatatt tatggtggct atcagtgcga
     2281 agcggcgttt ggcgaggcac tgaaactggc acctcacctg cgtgaacgga tggaaatcgt
     2341 cagtaaatgc ggtatcgcga cgaccgcgcg tgaagaaaac gtcattggtc attacatcac
     2401 tgaccgcgat cacatcatta agagcgccga acagtcgcta attaatctcg cgaccgatca
     2461 tctggatttg ctgttaatcc accgaccaga cccgttaatg gatgccgatg aagtggcgga
     2521 cgcgttcaaa catctgcatc agagcggcaa agtgcgtcat tttggcgtat cgaactttac
     2581 gcctgcgcaa tttgccctgt tgcaatcacg tctgccgttt acccttgcca ctaatcaggt
     2641 ggaaatatcc ccggtgcatc agccgttact gctggatggc acgctcgacc aactacaaca
     2701 actgcgtgtt cgtccgatgg cgtggtcctg ccttggtggt ggtcgtctgt ttaatgatga
     2761 ttatttccag ccgctgcgtg atgaactggc tgtggtggca gaggagttaa acgcgggctc
     2821 gattgaacag gtggtttacg cctgggtatt acgtttacca tcgcagccgc tgccaattat
     2881 cggttcaggt aaaattgagc gcgtacgggc agctgtcgaa gcagaaacac tgaaaatgac
     2941 ccgtcaacaa tggtttcgta tccgtaaagc ggcactgggg tacgacgtac cgtaagcact
     3001 tttaggaata gccgccgttc aaaaatgtgt cactggttta cacttattca ggaatgcaca
     3061 atgaacggag gtcctatgaa acgttttagt ctggctattc tggcgctggt tgttgcaacc
     3121 ggcgcacaag ctgccagtga aaaagtcgag atgaacctcg tcacgtcgca aggggtaggg
     3181 cagtcaattg gtagcgtcac cattactgaa accgataaag gtctggagtt ttcgcccgat
     3241 ctgaaagcat taccccccgg tgaacatggc ttccatattc atgccaaagg aagctgccag
     3301 ccagccacca aagatggcaa agccagcgcc gcggaatccg caggcgggca tcttgatcca
     3361 caaaataccg gtaaacatga agggccagaa ggtgccgggc atttaggcga tctgcctgca
     3421 ctggtcgtca ataatgacgg caaagctacc gatgccgtca tcgcgcctcg tctgaaatca
     3481 ctggatgaaa tcaaagacaa agcgctgatg gtccacgttg gcggcgataa tatgtccgat
     3541 caacctaaac cgctgggcgg tggcggtgaa cgctatgcct gtggtgtaat taagtaatta
     3601 cgaggccagc gtaccttgcg gtggtgcttg ctcaagttgc gaaagcgagc agtacagccg
     3661 ccagacaatt gccgccagct cacgggcggc aggttgatga tgacgggcaa ggctgttgca
     3721 aatccgctgt aattcttcaa gtgtggccgc cagtgatttt tgctgaacgc cacgctcact
     3781 catcactccc cgcaacagtg aaatacagtt atcccgtact cgcgataacg gatcggaacg
     3841 cgattcccag tcgcgcaatt gccagacaac atgagaacag ttcagcagca ctacacccca
     3901 gcgtaataac caacggcgag ccagcgcatc ctggctgtta ctcaactgac tgacgtgatg
     3961 ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta gctgatcgac
     4021 aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg atcctggacg
     4081 cagaatggcg aacgctagcc aagccagcgc aacgccaacg atttttgcca gattatcgtt
     4141 aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg aacccataaa
     4201 aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat
     4261 tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa tctggaccat
     4321 caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc gcatcagcag
     4381 tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa ttgctgccag
     4441 cgttaatgca ttggcaccgg catcccattg cgatgcaata ctccatgcgc caatcatcat
     4501 caacgtacaa aatgtacgca gcccgctcca catagcttcg gtgttgtcgg tatgacgagc
     4561 cagagcggat gtccgtgcga gtctggcatg atcatctaca ccgctttgca gacgatgtaa
     4621 ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc agaaggcgac
     4681 gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg ataatacgtg cgacggtgta
     4741 agcatctgtt tgcgaactgg cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc
     4801 acctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga cactggtcat
     4861 acgtaattgc tggtggagta gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg
     4921 gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa tcaccccctc
     4981 atgtgctgca cgaatggcat cgtttgtttc aggctgccag agtaaactgg catgttccag
     5041 taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg aactgctagg
     5101 caggatcatc atcatcatgc cgccgcacaa aataccgact atcacctcgc aaacgcgcgc
     5161 ctgagcgata tcccacagct ggctggcctc agtaatatta accatcggaa aggcgatgat
     5221 ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca
     5281 ggcccaggta caaaagccaa gccacgccga catgctcaat agaaaaaacc acggctcatt
     5341 gagcgtatgc ccggcaagaa gcagtgccgc aatggctccc agtaaactgc cagcgatgcg
     5401 tccgaggctt ttgctgatga caccgccaac ggtgggaaag ctaaccactg cagccgaggt
     5461 catcgcccaa tagggttcat ccagattcaa ataataggca accgtcagcg ccagacacat
     5521 ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc gtggccttga accagggcaa
     5581 attgcgcaag gaccatgacg atgcgttcat tattgaccta cggcaatgct acaggtggtg
     5641 cccgacacca gcgtgacatc gcctggaact ttatccagcg caaagcggac gggaacgcgt
     5701 tgggcgagac gaacccaggg gacgttaggt ttcacatccg gaataaggct tgagtcactt
     5761 tcaacgcttt gatcataaat cgcccgaccg atactggaaa cgtgaccccg taacgttttg
     5821 ttatcactat agagggtaat ttgtgctggt gcaccttcgc ggatatggcg caacttggtt
     5881 tcttcaaaat aaccaatgac gtaaaacgag tggctgtcga caagggcaaa cagcggtttc
     5941 ccggtgtcag cgtaatcgcc gatgcgggta gtgaggttag tcacccaacc actcaccggg
     6001 gcgcgaattt ccgtttgcgc cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca
     6061 ttaacactgg cctgcatcgc tttaacattg aggttagcgg tatcgagttc ttcggcagaa
     6121 ataaaatttt gtgagagatg acgacgacga ttcgcttcgt tattggcttt tgcgaggtca
     6181 gattgcgctt tcgccagctg agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta
     6241 tcgatcgtga gcagaagatc ccccgcgtta accagttggt tgtcttttat attcagctca
     6301 acaatacgtc ccgacacctg tggggtgata gaaacctttt ctgcgcgtat tttcccgtcg
     6361 cgtgtccacg gagattgcat gtaataattc cacagccacc atccggcaag aaccgcaact
     6421 atcgctacaa tgattgttga gaaatactta attgttttaa ttgacatagt taccacgcaa
     6481 tcagtattgc cagagcaagg caaacgcaaa tcgcaaacag cgataaatcc attaacaagg
     6541 gatgccagat gtcaccggcg tagatccagc cacgaagcag gcggtgtacg acaagccaga
     6601 tgacgaatcc aaacgcgaag gctttgaaaa acggaggaaa atagacggac gcaccgaaca
     6661 ccagatcttg taagggcaat cctgttgcat tgagcataaa cttcacaaag tagattcctt
     6721 tacgaccgaa tatattgcgt tagattaata aatattcttt aagtgcgaaa aatttacgcg
     6781 caatttctga aaaatatgtc tgacggtaac caaatgcagc aatacatttg ttttagcaat
     6841 acaattgctg cacactattc taaaagccgc ataatatctt agcaagctaa ttataaggag
     6901 atgaaattgg aatcgccact aggttctgat ctggcacggt tggtgcgcat atggcgtgct
     6961 ctgatagacc atcgcctgaa accgctggag ttaacacaaa cccattgggt tacgttacac
     7021 aatattcatc agttacctcc agaccagtcg caaattcaac tggcaaaagc gattggcatc
     7081 gagcagccat cactggtccg tactctggac caactggaag aaaaagggtt aatttcgcgt
     7141 caaacttgtg ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca
     7201 ctgatcagcg aaatggaagc tgttattaac aaaacccgcg cggaaatatt acatggcatc
     7261 tccgcagagg aactggagca actgattacg ctcatcgcaa aacttgagca taatatcatt
     7321 gagttacagg ccaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg accacacgta
     7381 aacttattta gcgcggagaa acggtcacct gactgccatt gctggccagt acgacacgtt
     7441 ggcccggaga gaaacgagtg ttgccttgtt tctgtaccac catgatggta ttaccatcgt
     7501 ctttacgaat ttccagctcg acaccctgcg ttttgttcat tgcactctgt acgccctgac
     7561 cagctacgcc acctgcaaca gcgcctgctg cagtagccag agaacgcccg gttccgccac
     7621 caacagtatt ccccaggaaa ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg
     7681 aatcatcacc gccctgaatc tgtaccggac gtacgttaac gatggtgcca tagctgacat
     7741 tctgtacttg tttcgcttca gaagcggtat aaacatcccc tgacagggtg tcgttattaa
     7801 cacaaccgac aagagacaga cctaccattg aaacaaccaa tacgcgttta atcattgaaa
     7861 aatctcctgt tcaccatgaa acgccacgca agcatccctc atggtcaaag tatatggcat
     7921 atgtgaatcc atggttacat gttctaccaa atatgagcaa atcataatga aagtaggatc
     7981 aaccaattat aaacaaaaca ttcatgatga ttattgattt gaagataatg ttttactacc
     8041 aacggtaaaa atcggtcatt ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg
     8101 caaacgtcag attgaaaaag gatgaactat gaaatcgggc cgctttattg gcgttatgtc
     8161 aggcaccagc cttgatggtg ttgatgttgt gttggcgaca atagatgaac accgggtcgc
     8221 acagctggca agcttgagct ggccgatccc ggtgtctctg aaacaggctg tactggatat
     8281 ttgccagggc cagcagctta cactttcgca gtttggacag cttgatactc aactcgggcg
     8341 actttttgct gatgcggtca atgccttgct taaggaacaa aacctgcagg cgagagatat
     8401 agttgcgatc ggctgtcacg gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca
     8461 cactttacag attggcgata acaatcaaat tgtggcacgc accggaatta cggttgtcgg
     8521 tgattttcgc cgtcgcgata ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt
     8581 ccatcatgcc ttgctggctc actcaaccga gcgacgaatg gtgctcaata ttggcggcat
     8641 cgccaatctg tcactgctca ttcctgggca gccggttggc ggctacgata ccggtcctgg
     8701 taacatgctg atggatgcct ggatctggcg tcaggccggt aaaccttacg ataaagatgc
     8761 cgagtgggca cgggcgggta aagttattct cccactgctg caaaatatgc tcagcgaccc
     8821 atatttctcg caacctgcac cgaaaagcac cggacgcgaa tactttaact acggctggct
     8881 ggagcgccat ttacgccatt ttccgggtgt tgatccccgt gatgttcagg ccacactggc
     8941 agaactcacc gccgtgacca tttctgaaca agttttgttg agcggtggct gcgaacgatt
     9001 gatggtatgt ggtggaggta gtcgtaatcc gctactcatg gcgcgtctgg cggcattact
     9061 gccaggcaca gaagtcacca ccaccgatgc cgttggcatt agtggcgatg acatggaagc
     9121 attggctttc gcctggcttg cctggcggac gctggcggga ttaccaggaa atctgccttc
     9181 cgtcactggc gcaagccagg agacggtact gggggctatt ttccctgcta acccgtgata
     9241 tcagagttaa ctgaattttt cttaccgcgt ctgccgttac acttggaacg ttagggaggg
     9301 cgtattgccc tccagaccag gaaagtcttc gggaaatgtc tatgaaaaaa ctgttactga
     9361 tctgtttgcc ggtgcttctc accggctgta gcgcgttcaa tcaactggtt gagcacatgc
     9421 aaaccgctac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc
     9481 cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa cagggcattt
     9541 cagcgtctgg tgcacgttac actgacggaa tctatgtttt ctggtcgaaa ggcgatgaag
     9601 cgactgtcta taaacgcgac cgcatcgtat tgaataactg tcagttacaa aatccacagc
     9661 gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa
     9721 accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa
     9781 ggcgggttac gccgtcgcga tcttcccgcc gatccattaa ccctttttga acgttggctc
     9841 tctcaggcgt gtgaagccaa actggcagac cctaccgcga tggtggtcgc taccgtggat
     9901 gaacatgctc agccttatca acgcatcgtt ttactcaaac attacgacga aaaaggcatg
     9961 gtgttttaca ccaacctcgg tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt
    10021 agcctgctgt tcccgtggca tacccttgaa cgccaggtga tggtgatcgg taaagcagaa
    10081 cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga tagccagatt
    10141 ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa
    10201 ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag cttttggggc
    10261 ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca tcgcctgcat
    10321 gaccgctttt tgtaccagcg agaaaatgat gcgtggaaga ttgatcgtct tgcaccctga
    10381 aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact ttattctatg
    10441 tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg
    10501 agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg ctggtagccc
    10561 aggtgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc gcgctctatt
    10621 gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca ttgttatgcc
    10681 tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc
    10741 tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa gaaactgttc
    10801 aggagtgggt ggacaaaatc cgtaagcagg ttgccccgtt cctcgatttc gactgtggag
    10861 aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat gtgctgacct
    10921 tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa gaagcggtta
    10981 agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc tacaacctgt
    11041 tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg caaattggtg
    11101 gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt ctgcatcaga
    11161 atcaggtgtt tggcctgacc gttccgctga tcactaaagc agatggcacc aaatttggta
    11221 aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac aaattctacc
    11281 agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc ttcaccttta
    11341 tgagcattga agagatcaac gccctggaag aagaagataa aaacagcggt aaagcaccgc
    11401 gcgcccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac
    11461 aggcggcaaa acgtattacc gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag
    11521 cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa aagggcgcag
    11581 acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa
    11641 ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct gaatacttct
    11701 ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt
    11761 actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga aatcactccc
    11821 cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc tcacgttgtt
    11881 tatggtcatg cgggtaacag tgcggcagag tttcctatgc gccgcctggg cgcgaacgtc
    11941 tggccgctga acaccgttca attttctaat cacacccaat acggcaaatg gactggctgc
    12001 gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgccat tgataaatta
    12061 cacacctgtg atgccgtgtt aagtggctat ctgggatcgg cggagcaggg tgaacatatc
    12121 ctcggtatcg tccgtcaggt gaaagccgcg aatccgcagg cgaaatattt ttgcgatccg
    12181 gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg
    12241 cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc
    12301 tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgtga actcattgcg
    12361 caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt
    12421 tttgaaatgc tgctggtcac cgccgatgaa gcctggcata tcagccgtcc gctggtggat
    12481 tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact gctggtgaaa
    12541 ctgcttcagg gggcaacgct gcaggaggcg ctggaacatg tgaccgctgc agtctacgaa
    12601 atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc tgctcaggat
    12661 cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg
    12721 cccacaacga cctgtgggcc gatggatatt actttaagcc ttccgctgac agcgcgtctt
    12781 gtacttccgg acgttcagcc atacgttgca taaatgctgc aatgtgctct aacccttcca
    12841 gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg
    12901 taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct
    12961 tcttctccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatca gggcgaaaca
    13021 gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat
    13081 agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat
    13141 actgcataat cgctacgcct tccgtcagca aagtaccgtc atccagcagc aatgcaggca
    13201 cctgcccctt agggttaacg acaaagtaat cgtcaccgtt ttcgagacgt tttttcatta
    13261 aatccacact gacgagggta aaatcctttc cgctctcacg cagggtgata tgggaagcga
    13321 gagagcaggc acccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta
    13381 aagtatggta gtgcgttgct gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt
    13441 aaaagagttt ccctgaattt tacgctacag cagctttcgc cgctttgtct gcagcgtcat
    13501 cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat cagtactgca ataatggcag
    13561 tagcgacacc aatctgcaag aatacgcgac cataaacttc cagtgacatc agcgggtcgg
    13621 taatgttatc cggcacagcc atcatacccg caacataacc accaatcagg tttgcaccgg
    13681 cagtggtcag gaaccagcta cccataatga agcccatcag acgctgcgga acgagttgtg
    13741 caaccattgc cagacccaga ccagagatca tcagttcccc gatgctctgc aggccatagc
    13801 ttgcgaccag ccagcttaca gacacgatac cagcgtcaga cgcgaatttc gcacccagcg
    13861 gcagaatcag gaacgcacca gaacacatca ccatgccgat tgcaaacttg gttggcatcg
    13921 gcagggtatc gcccatcttg ttatagatag cggccagaat cggactaccg atgatgatcc
    13981 agaacgggtt cagtgcctga tactgctcag gttctacggc cagacccaga atggagtgct
    14041 caacgttacg aatcgcaaag aagttcagtg acgttggcat ctggctgtac agcacgaaga
    14101 agataatggc ttcgagcatc aggatgaagg caacgatcat tttacgacgc gcagcacctt
    14161 tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc gaaggcaaca acgcccagcg
    14221 ccatacgcgc aacttcctga ttgtgcagca gccaggtggc gatagcgatc agtgccacaa
    14281 caccaataat ggtcagcagc aggttacggt agttgatagg ctcgaagtct ggttttgaac
    14341 cgtactgttt aacccagcgt tggcagaagg cgaagttaac gatagtgatc agcaggccta
    14401 caacgctcaa cgcaaacgca acactccagc cgtatttcgc ggccagccac ggcgtagcaa
    14461 tcatagagaa gaaagagccg atgttgacgg acatgtagta catggtgaat gcaccgtcca
    14521 gacgcgggtc gtttttctca tagcatgtag aaagcagaga agacgggtta gctttaaaca
    14581 ggccgttacc gaccgcaata gccgccatac ccatataaac gataccggcg tcgtgaccag
    14641 accaggcaac cagagcataa ccaatcgcca gcacaatagc gccgagcata attacgcgtt
    14701 tagtacccag taccttgtca cctaaccagc cgccgatagc gaccagacca taaaccaggg
    14761 cactaaagga agagaaaagg gtgattgaat ccgcttcaga catacccagt tgtttaacca
    14821 ggtaaacagc cataattcct tgtaggccgt aataaccaaa acgttcccat aactcaatcg
    14881 agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg
    14941 gtttttggtt tgcagtggac acttttacct ctttttttac atcccatatt aacgggggtg
    15001 ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt atagtgggag gggaaacggc
    15061 aggtaatgtt cactatcttg cactttcaga caagtccttt gtaataatct gttacatata
    15121 actgaggcgg ggaattcaac cattccggga aatgttatcc agcgttaaat ttttcaaaaa
    15181 acgtaactgc agattttttc actgcactga gagggggggg tgatggctta acggcgatat
    15241 gttctgctat ttagtgtttt tactagtgag atagtccagt ttctgaaaaa tagccagtgt
    15301 aatgttttgt aggtcaatat ttgttgcgtg tgtaaattta aatgatttta acctgattct
    15361 tgcaatgttt ttacattgcg acagcatcg
//
LOCUS       DAZFVO020000123        15255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFVO020000123 DAZFVO020000000
VERSION     DAZFVO020000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.124"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            182..1096
                     /gene="sitA"
                     /locus_tag="ACHKNB_RS17955"
                     /old_locus_tag="pgaptmp_003591"
     CDS             182..1096
                     /gene="sitA"
                     /locus_tag="ACHKNB_RS17955"
                     /old_locus_tag="pgaptmp_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HGU4197037.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            1096..1923
                     /gene="sitB"
                     /locus_tag="ACHKNB_RS17960"
                     /old_locus_tag="pgaptmp_003592"
     CDS             1096..1923
                     /gene="sitB"
                     /locus_tag="ACHKNB_RS17960"
                     /old_locus_tag="pgaptmp_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HGU4197038.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            1920..2777
                     /gene="sitC"
                     /locus_tag="ACHKNB_RS17965"
                     /old_locus_tag="pgaptmp_003593"
     CDS             1920..2777
                     /gene="sitC"
                     /locus_tag="ACHKNB_RS17965"
                     /old_locus_tag="pgaptmp_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HGU4197039.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            2774..3631
                     /gene="sitD"
                     /locus_tag="ACHKNB_RS17970"
                     /old_locus_tag="pgaptmp_003594"
     CDS             2774..3631
                     /gene="sitD"
                     /locus_tag="ACHKNB_RS17970"
                     /old_locus_tag="pgaptmp_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HGU4197040.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(3865..4122)
                     /locus_tag="ACHKNB_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /pseudo
     CDS             complement(3865..4122)
                     /locus_tag="ACHKNB_RS17975"
                     /old_locus_tag="pgaptmp_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4120..4533
                     /locus_tag="ACHKNB_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /pseudo
     CDS             4120..4533
                     /locus_tag="ACHKNB_RS17980"
                     /old_locus_tag="pgaptmp_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            4874..5254
                     /gene="crcB"
                     /locus_tag="ACHKNB_RS17985"
                     /old_locus_tag="pgaptmp_003597"
     CDS             4874..5254
                     /gene="crcB"
                     /locus_tag="ACHKNB_RS17985"
                     /old_locus_tag="pgaptmp_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4197041.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(5634..6827)
                     /locus_tag="ACHKNB_RS17990"
                     /old_locus_tag="pgaptmp_003598"
     CDS             complement(5634..6827)
                     /locus_tag="ACHKNB_RS17990"
                     /old_locus_tag="pgaptmp_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4197042.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            6963..8687
                     /gene="iucA"
                     /locus_tag="ACHKNB_RS17995"
                     /old_locus_tag="pgaptmp_003599"
     CDS             6963..8687
                     /gene="iucA"
                     /locus_tag="ACHKNB_RS17995"
                     /old_locus_tag="pgaptmp_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HGU4197043.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            8688..9635
                     /gene="iucB"
                     /locus_tag="ACHKNB_RS18000"
                     /old_locus_tag="pgaptmp_003600"
     CDS             8688..9635
                     /gene="iucB"
                     /locus_tag="ACHKNB_RS18000"
                     /old_locus_tag="pgaptmp_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HGU4197044.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            9635..11377
                     /gene="iucC"
                     /locus_tag="ACHKNB_RS18005"
                     /old_locus_tag="pgaptmp_003601"
     CDS             9635..11377
                     /gene="iucC"
                     /locus_tag="ACHKNB_RS18005"
                     /old_locus_tag="pgaptmp_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HGU4197045.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            11374..12707
                     /gene="iucD"
                     /locus_tag="ACHKNB_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /pseudo
     CDS             11374..12707
                     /gene="iucD"
                     /locus_tag="ACHKNB_RS18010"
                     /old_locus_tag="pgaptmp_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            12733..14934
                     /gene="iutA"
                     /locus_tag="ACHKNB_RS18015"
                     /old_locus_tag="pgaptmp_003603"
     CDS             12733..14934
                     /gene="iutA"
                     /locus_tag="ACHKNB_RS18015"
                     /old_locus_tag="pgaptmp_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HGU4197046.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         3657 a         3848 c         3920 g         3830 t
ORIGIN      
        1 ttaaagccaa agcgctgccc aacttttatc ctcaatacca catcgtcagc aggaaataac
       61 ctaatccctg tgttgggatc ttataatcgc taatgataat tatttacatt ttatagcttc
      121 tgctataaaa catagtagtt gctatgttta ttctaatttt gctcactata ggtactaaat
      181 catgctctca ataaaaaaag taaccatgct cttggggtgc ctagtcctca cctgctcgat
      241 agcatttcag gcgagtgcag ctgaaaaatt caaggtcatt acaactttta ccatcatcgc
      301 agatatggcc aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa ccaagcctgg
      361 tgcagaaatt catgagtatc agcctacccc tggcgatatt aaacgtgcgc agggggcaca
      421 actgattctc gccaatggta tgaatctgga attgtggttc caacgctttt accagcatct
      481 caatggggtt ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga tcaccgaagg
      541 gccctatgag ggcaaaccta acccccatgc ctggatgtcg ccagataatg ctctgattta
      601 cgtcgataat attcgtgatg cgtttataaa atacgatccg gcaaatgcac aaacctacca
      661 acgtaatgcc gatacttata aagccaagat tacccaaacc cttgcccccc tgcgtaagca
      721 gattgcggaa ctccctgaga atcagcgatg gatggtcacc agtgaagggg ctttttctta
      781 tctcgcacgc gatttggggc taaaagagct ttatctgtgg ccgattaatg ccgatcaaca
      841 aggaacaccg cagcaggtac gtaaggttgt tgatatagtt aagaaaaatc atatcccggc
      901 agtctttagc gagagtacga tttccgataa accagcgcgt caggttgcgc gtgaaaccgg
      961 cgcgcactac ggtggtgtgc tctatgtcga ttccctgagc acagaaaacg gcccggtacc
     1021 tacgtatatc gaccttctga aggttactac cagtacgctg gtacagggaa ttaaagccgg
     1081 aaaaagggag aaataatgat gcaatctgca ggcattgtcg ttaatgatgt tactgtcacc
     1141 tggcgtaacg ggcacacagc actgcgcgat gcatccttca cagtaccagg cggatctatt
     1201 gccgctctgg ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc gattatggga
     1261 tttgtgcgtc tgaccagcgg gaagatatct gttctgggta ttcccacacg acaggcgcta
     1321 cagaaaaacc tggttgccta cgtccctcag tcagaagagg ttgactggtc atttcctgta
     1381 ctggtggaag atgtggtgat gatgggccgc tatgggcata tgggtatgtt gcgtatcgcc
     1441 aaaaagaggg atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat ggtggatttt
     1501 cgccatcgac aaatcggcga gctttccggt ggacaaaaga aacgcgtctt tctggcgaga
     1561 gcgattgcac agcagggtga tgtgatcctg ctggatgaac cttttaccgg cgttgatgtg
     1621 aaaaccgaag cgaaaatcat cagcctgttg agggagttac gcgcggaagg caaaacgatg
     1681 cttgtctcaa cgcacaatct cggatccgtc acgacatttt gcgattatac ggtcatggtc
     1741 aaaggcaccg tcctggcaag cgggccgaca gacaccactt ttacagccga gaacctggag
     1801 ctggctttta gcggcgtact gcgccatgtc acacttaacg gctcggaaga aagcattatt
     1861 actgacgatg aacgcccttt tgtggcacat cgaccgtcag cggtgcagag ggaagaaaga
     1921 tgaacgtgct actggaaccc ttcagctatg aatatatgct caatgcgatg tgggtctcgg
     1981 cgatggtcgg cggtctctgc gctttcctgt catgctatct gatgctaaaa ggctggtcgc
     2041 tgattggcga tgcgctgtcg cactctattg tcccgggtgt tgcgggggca tatatgctgg
     2101 ggcttccgtt ttctctggga gccttctttt ccggcggact ggcagcgggc agcatgctgt
     2161 ttcttaacca actcactcgc ttaaaagaag atgccatcat cggcctgatc ttctcgtcct
     2221 tttttggcct ggggctgttt atggtgtcat tgaacccgac atccgtgaac attcagacca
     2281 tcgtcctggg caatattctg gcaatcgatc cggcggatat cctgcaactc actatcattg
     2341 gcattctctc gatcatcgtg ctttttttca aatggaaaga tcttatggtg acctttttcg
     2401 acgagaatca cgcccgtgct attggattgc atccgggcag attaaaaatc ctcttcttca
     2461 ccctgctgtc ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt ctggtgatct
     2521 gtctggtggt gactcccggt gcaacagcat ggttgttaac cgatcgtttt ccccgcctgc
     2581 tcatgattgc tgtaactatt ggcagtgtga ccagtttcct cggcgcgtgg gtcagttact
     2641 ttctggacgg cgcgacaggt ggaatcatcg tggtggcgca gacactgctg tttcttttag
     2701 catttgtctt tgcgcccacc catgggcttt tggcaaatcg tcggcgtgcg cataaagcgc
     2761 tggaggatcg ctcatgatgg cattattact ggagccactg cagttcacct ttatgagtca
     2821 cgcactgctg atttcactgg ttgtttccat accctgtgcg ctgctgtcgg tctttctggt
     2881 cctgaaaagt tgggcactga tgggtgacgc gatgagtcat gcggtttttc ctggcatcgt
     2941 gcttgcctgg attctggggt tacctctggc tacaggggca ttcgtcgccg gagtattctg
     3001 tgcggtggcg acgggatacc tgaaagacaa cagccgaatc aagcaagata cagtgatggg
     3061 gattgttttt tctggcatgt ttgccgcagg tctgatcttg tatattgcag tcaaaccaga
     3121 cgtacatctt gatcatattc ttttcggcga catgctcggg ataaccatcg gcgatataat
     3181 ccagacggtg attattgccg gactggttac acttgttatt agcgtaaaat ggcgcgattt
     3241 tttgctgttc agctttgatt atcaacaggc gcaggcaagc ggtttgcata cgagatggct
     3301 gcattatggg ctgctgtgca tggtctccct gaccattgtg gcgacgctga aagcggtagg
     3361 tatcattctc tctatttctc ttctcattgc gcctggtgcc attgcggtac tcctgacaca
     3421 acgctttcat attgcgctcc tgctggcgac tggcatatcg atattggtct caatgactgg
     3481 tgtctggctg tcctttttta tcgacagcgc gccagctccg acaattgttg tcttattcgc
     3541 ggtcatgttc atcatgacgt ttaccgttac cagcatcaac gcacgcacaa aggaaaatgc
     3601 cgaaccacgg gatttgttat catccgatta gcagaacact cggccagcaa catccgctta
     3661 ttggcgatgc gcttgaacga ataatgcaca gacttatgat tctgataagt tttgttgcca
     3721 gagagttgct gcggactgga tagcaagtaa tgtcgattct caggggtagc aaacagcgca
     3781 gtaaaatgag ggaagtagtg gcaaaacata gagaaaattg gggggcgagg gccagtgatg
     3841 taaaaaacca cgctaagagt ttaattaatt gtttgtattg aagtttaatg atcttaactc
     3901 acccttaaga atatcttccg gtaacgaaga cgtataatgc cccagcatat ttatatggct
     3961 atgtatcagc agcgataatc ttgccggatg ctactcctca ggagtttctc ccgatttgcg
     4021 aagatgtgac aacgcctcct gcatgtaaag tgtgtttcac agcacaaccg cattggtcac
     4081 cagccccaga gcacctagtt gatcttcctg cccttaacgg tagctgaaga tgactgggct
     4141 ggatggaaaa tcctgcatgg tgcattggga gagcagatag aattggttgg cgacgatctg
     4201 tttgtgacca acgtaaagta tattcagcgc ggcattgacg aaaaccttgc caattcagca
     4261 ttgattaaac tcaaccagat tggttccctg agtgaaacct ttgatgcggt acaactttgc
     4321 catgacaaca actggggcac ctttatctca caccgcagtg gtgagacagt ggacagcttt
     4381 atcgccgata tgacagttgc aatgcgcgca ggtcatctca aaacgggagc tccatgccgg
     4441 ggtgagcgta ttgaaaaata taaccagctt atgagaattg aggatgaact gggttcatcg
     4501 gcgcaatttg ccggaaagtc tgcctttaaa tgacattatt gtgccaatcc cccaggctta
     4561 cacgtcgggg ttattctgga ggaatgatga aagctcacgt ttttgttatt tctatgctta
     4621 ctggcattgt cgtcacctat gccgtgttac ttcttggttg cttgttcatc gataaaacgc
     4681 tccccactgt agatgttgtc attctgtcac ttgtcgtggg ggcttctgct cagcaattgt
     4741 cgagggtatt aatgtcaata aatagaacct tcccttatct tgcatgtagt caaaggatat
     4801 aaattaacaa tatcagtcaa atgagaacat attctgatat tgttacgata aaaaattatt
     4861 ttatgagggg tacatgatta aatcactttt tgccgtcatt atcggcggtt ctgttggatg
     4921 tactctgcgc tggttactgt ccacacgctt taacagtctt tttccaaatc tacctccagg
     4981 tacactggtg gtgaacctcc ttgccgggtt aattattggt actgcgttgg cgtacttttt
     5041 acgacaacca caccttgatc ctttttggaa attaatgata acaacgggac tttgtggagg
     5101 tctgagtact ttctctacat tttctgttga agtattcgca ttactccagg cgggaaatta
     5161 tatatgggct ttgacgtctg tacttgttca cgtcattggc tccctcatca tgacagcttt
     5221 aggctttttt atcataacca tacttttcgc atgagaattc attaacgtta caggtttatc
     5281 aactaatgtg acgcttagga tagaatttcg ctccatggta tgcgtttgca gtcattacag
     5341 tctccatgac gactgtaatg actgcaaacg cattacctga catatcagta taaacaatga
     5401 attaacctct tggcgtattt tttacatcac tggccctcac cccccaaaat tctctctgaa
     5461 aaattgttgc ggttaacgtt gccaatgtat ccacccattt cagaagacga tctggcatcc
     5521 tggtggcatg ctgcgatgtg cttcaggtga gtgcactggg cggtcagcac aggcggtttc
     5581 gacagcgaaa ttgcacagga cgttatgcca gctgtgagta caacatcata gccttacttg
     5641 attgtacggt aatgccaaat acatgcagac actatggtaa ataatacagc ggcaaataat
     5701 gccgtcacaa tcagcgcgat tgagacacct gttttcgacg ccagtggcac caacaatgcg
     5761 cacagcccat agccaagtgt gtgactggcc gcaagccagc ccgcgcctgc gccgtcggcc
     5821 agcttatcat tcagtaaaag ttgataagcg ggtgttgcca gagcggcccc aaatgacagc
     5881 actgcacagc ccaggtaaaa caaccatagc tgttcagtga gcatgatagc cagaccacta
     5941 ctcatcaaca ccccggcact caggagcaga gccactggag tcaggcgctg tggacggagt
     6001 accacgaact gcgcgataag cgcagctatt gcggacagcc ccaacaacca cgccacctgt
     6061 tggctaatag tggtggtatc agtatcgaac tggcgagtaa gggctggcga aagtccaagc
     6121 tgcatcatgc tcattgccgc agccagtaac attgcgcaaa gcagataagg caggcaatcc
     6181 cgcttcaggc tgacgctctt gcgctccggt gtgggctgtg gtggtgtgcc gggcaaccgc
     6241 agaagcatca ccacagccca caccggagct gccatcagca tccacactgg cgccagaggg
     6301 tgaatgacca acattgccgc cgcgcacagc ggcccgaata gcctgccgca gctcaggccg
     6361 gagctgatgg ttgccagagc ggccatgcga ttcccttctc ccgctctctg caatgcccag
     6421 acctgacagg ctggcaccat tgctgacacg gtcagaccgt agacgatccg ggcgatgatt
     6481 aatattccca accccaccgc tgttgttacg gcaccggtag ccatcagcac gcttcccagc
     6541 ccaagcaggg taaagcttac gccatatcct cccagagccc acagcaccac agtcttactg
     6601 ccagtaaggg aaatttgctt tccccaccat ggggaagatg gcagaaacag cattgagccc
     6661 agcatcagca gggcagccca tacagacaga ctcagatttg tctgtatgac cagaacaggg
     6721 agtacaacca gcagaccgtt ctgtccgata ccgagaagac cggcactgaa cgccagtggc
     6781 cagcaggaca gggttttttg gggcgtatct tcgatcttag atgacattgt aatgtttcaa
     6841 caccatgtat taattgtgtt tatttgtaaa attaattttt ctgacaataa catttctcat
     6901 tgataatgag aatcattatt gacataattg ttattatttt actgtgtagg agctgtttga
     6961 ttatgatcct gccctctgaa aaatccgcca cagatgtggc tgcgcagtgc ttcctgaatg
     7021 ccttgattcg tgaaaccaaa gactggcaac tggcggaata cccgccagac gaattgatta
     7081 tcccgctgga cgaacagaag tcgctccatt tcagagtggc ttatttctcc ccaacccagc
     7141 atcaccgctt tgcatttcct gctcatctgg tcacggcatc aggcagttat cctgtcgact
     7201 ttaccactct ctcccggctt attattgata agctccggca tcaacttttt ctgccagttc
     7261 ccctctgcga aactttccac caacgcgtgc tggaaagcta cgcccatacg caacagacaa
     7321 ttgatgcccg ccatgactgg gccatcctgc gtgaaaaagc gttgaatttt ggcgaggctg
     7381 agcaggcact gctgacagga cacgctttcc accctgcacc taagtctcat gaaccgttta
     7441 accgccagga agctgaacga tacctgcctg acatggcacc tcacttcccg ctgcggtggt
     7501 tttcggtgga taaaacgcaa atcgctggtg aaagtctgca tcttaacctt caacagcggt
     7561 tgacgcgatt tgccgcagaa aatgcgcccc agttactcaa cgaattaagt gacaatcaat
     7621 ggctgttccc gctgcacccg tggcagggag aatatctttt gcagcaagtg tggtgccagg
     7681 cactttttgc taaaggactt atcagagact taggcgaggc cggcacgtcg tggctgccga
     7741 ccacctcttc ccgctccctc tactgtgcta ccagccgcga tatgatcaag ttctccctga
     7801 gcgttcggct gaccaactcc gtccgtactc tgtctgtgaa agaggtggag cgaggaatgc
     7861 gcctggcacg tctggcgcaa accgacggct ggcagatgct acaggcccgc ttccctactt
     7921 tccgggtaat gcaggaggac ggctgggccg ggctgcgcga tcttaacggc aacatcatgc
     7981 aggaaagttt gttttccctg cgtgaaaacc tgctgctgga gcagccgcaa agccagacca
     8041 acgttctggt ttccctgact caggccgcac cggatggcgg tgattcgctg ctggtttcgg
     8101 cggtaaaacg cctgagcgat cgcctcggta tcactgtgca acaggccgcc cacgcatggg
     8161 tcgatgctta ctgtcagcag gtgctgaagc ctctgtttac ggctgaagcg gattacggcc
     8221 tggtgctgct ggcgcatcag caaaatattc ttgtgcagat gcttggggat ctgccggtcg
     8281 gatttattta ccgtgactgc cagggcagcg cgtttatgcc tcacgcgaca gagtggctcg
     8341 ataccattga cgaggcgcag gcggaaaata ttttcacccg tgagcagttg ctgcgctatt
     8401 tcccttatta cctgctggtt aactccactt ttgccgtaac tgctgcgctg ggtgctgccg
     8461 ggctggacag cgaagcgaat ctgatggctc gtgtacgaac attgctggct gaagtgcgtg
     8521 accaggtgac tcataaaacc tgtctcaact atgttctgga aagtccgtac tggaacgtaa
     8581 aaggtaactt tttctgttat ctgaacgatc ataacgagaa caccatcgtt gacccttcgg
     8641 tgatctattt tgatttcgct aacccgctgc aggctcagga ggtctgaatg tctggggcaa
     8701 acattgttca cagcggatat ggactgcgct gtgaaaaact cgacaagcct ctgaatcttg
     8761 gctgggggct ggacaatagc gcggtgttgc actggcccgg ggagctgcca acagggtggc
     8821 tgtgcgacgc gctggatcag atatttatcg ccgcaccaca actttcagca gtggttcttc
     8881 cctggtccga atggtgtgag gagccacagg cgctgacgct tttcggacag gtacaaagcg
     8941 acattatcca tcgttccgct ttctggcagt taccgttatg gctgagttct ccggcaaacc
     9001 gggcctccgg tgaaatggtt tttgatgcag agcgtgagat ttatttcccg cagcgccccc
     9061 cccgtccgca gggtgaagtt tatcgtcgtt acgatccacg cattcgcagg atgctgagtt
     9121 tccgcattgc cgatcccgtt tctgatgcag aacgtttcac tcgctggatg aacgatccgc
     9181 gcgttgagta tttctgggag caaagtggct cactggaggt acagatcgcc tatctggaac
     9241 gccagctcac cagtaaacat gcattcccgc tgattggttg tttcgacgat cggccgttca
     9301 gttatttcga aatctactgg gcggcggaag accgcattgg tcgccactat tcgtggcagc
     9361 cctttgaccg tggcctgcat ctgctggttg gtgaacagca atggcgcgga gcccactatg
     9421 tgcaaagctg gctgcgcggg gtgacacatt acctgctgct gaatgagcca cgtacgcagc
     9481 gcacagtact ggagccacgc accgataacc agcgcctgtt ccgccatctt gagcctgcgg
     9541 gataccggac aattaaagag ttcgacttcc cacataaacg ctcgcgcatg gtgatggcgg
     9601 atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat cacaaagact gggatttggt
     9661 caaccgccgc ctggtggcaa aaatgttgtc tgagctggag tatgagcagg ttttccacgc
     9721 ggaatctcag ggcgatgacc gctactgcat taacctgccg ggagcacaat ggcgcttcat
     9781 cgctgaacgt ggtatctggg gctggctctg gattgatgct caaactctgc gctgcgcgga
     9841 cgagccagta ctggctcaga cgctgctgat gcagctaaag caggtactgt caatgagcga
     9901 tgcaaccgtt gctgagcata tgcaggattt gtatgccacg ctgctgggcg acctgcaact
     9961 actgaaagcc cgtcgcgggc tgagcgccag tgacctgatt aatcttaatg ccgaccgcct
    10021 gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat aaaggtcgcc gtggctgggg
    10081 taaagaggcg ctggaacgat atgcgccaga gtatgccaac accttcagac tgcactggct
    10141 ggcggtaaaa cgtgaacata tgatctggcg ctgtgataac gagatggata ttcatcagtt
    10201 gttgacggcc gcaatggatc cgcaggagtt tgcccgcttc agtcaggtct ggcaggaaaa
    10261 cggactggat cataactggc tgccgctgcc ggtacatccg tggcagtggc agcaaaaaat
    10321 cgctaccgac ttcatcgctg attttgccga aggcaggatg gtgtctctcg gcgagtttgg
    10381 cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc aacgccagtc gccggggcgg
    10441 gctggatatc aagctgccgc tgaccatcta caacacctca tgctaccggg ggatccctgg
    10501 cagatacatc gctgccggac cactggcttc acgctggcta caacaggttt ttgcgaccga
    10561 cgccacccta gtgcaaagcg gcgcagtgat acttggtgaa ccggctgcag gctatgtctc
    10621 ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc taccaggaaa tgcttggtgt
    10681 tatctggcgg gagaatccgt gccgctggct gaaaccggat gaaagtccgg ttctgatggc
    10741 aacactgatg gagtgcgacg aaaacaatca gccgctggca ggcgcatata tagaccgctc
    10801 cgggctggac gctgaaacct ggcttacgca actgttccgg gtggtcgtgg ttcctctgta
    10861 tcacctgctt tgccgctacg gtgtcgcgct tattgcacat ggacaaaata taactctcgc
    10921 catgaaagag ggggttccac agcgtgttct gctgaaagac ttccagggcg atatgcgact
    10981 ggtgaaagaa gagttccccg aaatggactc tttgcctcag gaggttcgtg atgttacatc
    11041 ccgcctgagt gcggactact taatccatga tttgcagacg ggtcacttcg tgacagtact
    11101 gcgttttatt tcgccactga tggttcgtct tggcgtacct gaaaggcgat tttatcaact
    11161 gctggcagca gtgttgagtg attacatgaa aaaacatcca caaatgtcag agcgttttgc
    11221 gcttttctca cttttcaggc cacaaatcat tcgcgtggtg ctgaacccgg taaaactgac
    11281 ctggcctgat ctggatggcg gcagccgcat gctgccgaat taccttgagg atctgcaaaa
    11341 tccgctgtgg ctggtaactc aggaatatga atcatgaaaa aaagtgtcga ttttattggt
    11401 gtagggacag ggccatttaa tctcagcatt gctgcgttgt cacatcagat cgaagaactg
    11461 gactgtctct tctttgatga acatcctcat ttttcctggc atccgggtat gctggtaccg
    11521 gattgtcata tgcagaccgt ctttctgaaa gatctggtca gtgctgttgc acctacaaat
    11581 ccctacagtt ttgttaacta tctggtgaag cacaaaaagt tctatcgctt ccttacaagc
    11641 agactacgta cagtatcccg tgaagagttt tctgactacc tccgctgggc tgctgaagat
    11701 atgaataacc tgtatttcag tcataccgtt gaaaacattg atttcgataa aaaacgtcga
    11761 ttgtttctgg tgcaaaccag ccagggagaa tattttgccc gcaatatctg ccttggtaca
    11821 ggaaaacaac cttatttacc accctgtgtg aagcatatga cacaatcctg tttccatgcc
    11881 agtgaaatga atcttcgtcg gccggacctt agtggaaaac ggataaccgt ggttggtgga
    11941 ggacagagtg gtgcagacct gttccttaat gcattacgcg gggaatgggg agaagcggcg
    12001 gaaataaact gggtctcccg gcgtaataat tttaacgcac tggatgaggc tgcttttgct
    12061 gatgagtatt ttacacctga atatatttca ggcttctccg gactggagga agatattcgc
    12121 catcagttac tggatgagca gaaaatgaca tcggatggca tcactgccga ttctttactg
    12181 accatttatc gtgagttgta ccaccgtttt gaagttctga gaaaaccaag aaatatccgt
    12241 ctgctaccca gccgctcggt aacaactctg gaaagtagtg gtcctggctg gaagttgctg
    12301 atggagcatc atctggatca gggcagggag agcctggaaa gtgatgtggt gattttcgcc
    12361 acaggttacc gttctgcgtt gccacaaata cttccctcac tgatgcccct gatcaccatg
    12421 cacgataaga acacctttaa agtgcgtgat gacttcactc tggaatggag tggcccgaaa
    12481 gagaacaaca tcttcgtggt caacgccagt atgcaaaccc atggcatcgc cgaaccccag
    12541 ctcagcctga tggcatggag atctgcacgt attcttaatc gcgtaatggg acgtgattta
    12601 ttcgatctca gtatgccgcc cgccctgatt cagtggcgca gcggcaccta gggaaaacgc
    12661 agccggaggc tgcttcttta actcgctaca cagcatcttt gggctgattt tttccgcccg
    12721 tatggaggaa taatgatgat aagcaaaaag tatacgcttt gggctctcaa cccactgctt
    12781 cttaccatga tggcgccagc agtcgctcaa caaaccgatg atgaaacgtt cgtggtgtct
    12841 gccaaccgca gcaatcgcac cgtagcggag atggcgcaaa ccacctgggt tatcgaaaac
    12901 gccgaactgg aacagcagat tcagggcggc aaagagctta aagacgcact ggctcagctg
    12961 atccctggcc ttgacgtcag cagccggagc cgcaccaact acggtatgaa tgtgcgtggc
    13021 cgcccgctgg tcgtgctggt tgacggcgtg cgtctcaact cttcacgtac cgacagccga
    13081 caactggact ctatagatcc ttttaatatc gaccatattg aagtgatctc cggtgcgacg
    13141 tccctgtacg gcggcggcag taccggtggc ctgatcaaca tcgtgaccaa aaaaggccag
    13201 ccggaaacca tgatggagtt tgaggctggc accaaaagtg gctttagcag cagtaaagat
    13261 cacgatgaac gcattgccgg agctgtctcc ggcggaaatg agcatatctc cggacgtctt
    13321 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccttgctt
    13381 gataacaccc agaccggcct gcagtactcc gatcggctgg acatcatggg aactggtacg
    13441 ctgaacatcg atgaatcccg gcagcttcag ttgatcacac agtactataa aagccagggc
    13501 gacgacgatt acgggcttaa tctcgggaaa ggcttctctg ccatcagagg gaccagcacg
    13561 ccattcgtca gtaacgggct gaattccgac cgtattcccg gcactgagcg gcatttgatc
    13621 agcctgcagt actctgacag cgcttttctg ggacaggagc tggtcggtca ggtttactac
    13681 cgcgatgagt cgttgcgatt ctacccgttc ccgacggtaa atgcgaacaa acaggtgacg
    13741 gctttctctt cgtcacagca ggacaccgac cagtacggca tgaaactgac tctgaacagc
    13801 aaaccgatgg acggctggca aatcacctgg gggctggatg ctgatcatga gcgctttacc
    13861 tccaaccaga tgttcttcga cctggctcag gcaagcgctt ccggagggct gaacaacaag
    13921 aagatttaca ccaccgggcg ctatccgtcg tatgacatca ccaacctggc ggccttcctg
    13981 caatcaggct atgacatcaa taatctcttt accctcaacg gtggcgtacg ctatcagtac
    14041 actgaaaaca agattgatga tttcatcggc tacgcgcagc aacggcagat tgccgccggg
    14101 aaggctacat ccgccgacgc cattcctggc ggctcagtcg attacgacaa cttcctgttc
    14161 aacgccggtc tgctgatgca catcaccgaa cgccagcagg catggctcaa cttctcccag
    14221 ggcgtggagc tgccggaccc gggtaaatac tatggtcgcg gcatctatgg tgctgcagtg
    14281 aacggccatc ttcctctaac aaagagtgtg aacgtcagcg acagcaagct ggaaggcgtg
    14341 aaagtcgatt cttatgagct gggctggcgc tttactggca ataatctgcg tacccaaatc
    14401 gcggcctact attcgatttc tgataagagc gtggtggcga ataaagatct gaccatcagc
    14461 gtggtggacg acaaacgccg tatttacggc gtggaaggtg cggtggacta cctgattcct
    14521 gatactgact ggagtaccgg agtgaacttc aacgtgctga aaactgagtc gaaagtgaac
    14581 ggtacctggc agaaatacga tgtgaagaca gcaagcccat caaaagcgac agcctacatt
    14641 ggctgggcac cggacccgtg gagtctgcgc gtgcagagca ccacctcctt tgacgtgagc
    14701 gacgcgcagg gctacaaggt cgatggctat accaccgtgg atctgctcgg cagttatcag
    14761 cttccggtgg gtacactcag cttcagcatt gaaaacctct tcgaccgtga ctacaccact
    14821 gtctgggggc agcgtgcacc actgtactac agcccgggtt acggcccagc gtcactgtac
    14881 gactacaaag gcaggggccg aacctttggt ctgaactact ctgtgctgtt ctgaccggta
    14941 ttcctttaca acaaaggtac gctgatatca acatggccgc tgacagccaa gttgatatca
    15001 tataatacac gacataatct gtagtcaggg aggatagact ctttactgac tacagattat
    15061 gtcgtgttcc gtgctcattt cctcaaaaaa atacaagaaa agaattagta ttctaacaaa
    15121 aagtgaaata aattgtatca aactccctct tttaatcctg ttgagtaaat cagcttttgc
    15181 aataggattg aaagagtgta agtggaatct cttccggata ctcgttacca ccgtggctag
    15241 aatatctacg gctgc
//
LOCUS       DAZFVO020000124        14832 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFVO020000124 DAZFVO020000000
VERSION     DAZFVO020000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.125"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..108)
                     /locus_tag="ACHKNB_RS18020"
                     /old_locus_tag="pgaptmp_003604"
     CDS             complement(<1..108)
                     /locus_tag="ACHKNB_RS18020"
                     /old_locus_tag="pgaptmp_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4197047.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGY"
     gene            complement(252..929)
                     /gene="tli1"
                     /locus_tag="ACHKNB_RS18025"
                     /old_locus_tag="pgaptmp_003605"
     CDS             complement(252..929)
                     /gene="tli1"
                     /locus_tag="ACHKNB_RS18025"
                     /old_locus_tag="pgaptmp_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HGU4197048.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDNTEPSQVSVGRWSDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMKMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(949..2631)
                     /gene="tle1"
                     /locus_tag="ACHKNB_RS18030"
                     /old_locus_tag="pgaptmp_003606"
     CDS             complement(949..2631)
                     /gene="tle1"
                     /locus_tag="ACHKNB_RS18030"
                     /old_locus_tag="pgaptmp_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HGU4197049.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNNISNGSYRGYYSNI
                     HWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGKEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(2628..5147)
                     /locus_tag="ACHKNB_RS18035"
                     /old_locus_tag="pgaptmp_003607"
     CDS             complement(2628..5147)
                     /locus_tag="ACHKNB_RS18035"
                     /old_locus_tag="pgaptmp_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HGU4197050.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATLTMGTGGLKGQVNQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(5450..6091)
                     /locus_tag="ACHKNB_RS18040"
                     /old_locus_tag="pgaptmp_003608"
     CDS             complement(5450..6091)
                     /locus_tag="ACHKNB_RS18040"
                     /old_locus_tag="pgaptmp_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197051.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(6078..8765)
                     /gene="tssH"
                     /locus_tag="ACHKNB_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /gene_synonym="clpV"
     CDS             complement(6078..8765)
                     /gene="tssH"
                     /locus_tag="ACHKNB_RS18045"
                     /old_locus_tag="pgaptmp_003609"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HGU4197052.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAQSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRIIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWHDEDGIVIAFDGINSDYPQESIYGQC"
     gene            complement(8933..9424)
                     /gene="hcp"
                     /locus_tag="ACHKNB_RS18050"
                     /old_locus_tag="pgaptmp_003610"
     CDS             complement(8933..9424)
                     /gene="hcp"
                     /locus_tag="ACHKNB_RS18050"
                     /old_locus_tag="pgaptmp_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HGU4197053.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(9459..11159)
                     /locus_tag="ACHKNB_RS18055"
                     /old_locus_tag="pgaptmp_003611"
     CDS             complement(9459..11159)
                     /locus_tag="ACHKNB_RS18055"
                     /old_locus_tag="pgaptmp_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HGU4197054.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     ARPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP
                     "
     gene            complement(11162..11815)
                     /gene="tssL"
                     /locus_tag="ACHKNB_RS18060"
                     /old_locus_tag="pgaptmp_003612"
     CDS             complement(11162..11815)
                     /gene="tssL"
                     /locus_tag="ACHKNB_RS18060"
                     /old_locus_tag="pgaptmp_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HGU4197055.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRVGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            complement(11812..13149)
                     /gene="tssK"
                     /locus_tag="ACHKNB_RS18065"
                     /old_locus_tag="pgaptmp_003613"
     CDS             complement(11812..13149)
                     /gene="tssK"
                     /locus_tag="ACHKNB_RS18065"
                     /old_locus_tag="pgaptmp_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HGU4197056.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLYHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            complement(13165..14709)
                     /gene="tssC"
                     /locus_tag="ACHKNB_RS18070"
                     /old_locus_tag="pgaptmp_003614"
     CDS             complement(13165..14709)
                     /gene="tssC"
                     /locus_tag="ACHKNB_RS18070"
                     /old_locus_tag="pgaptmp_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HGU4197057.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIWGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            complement(14730..>14832)
                     /locus_tag="ACHKNB_RS18075"
                     /old_locus_tag="pgaptmp_003615"
     CDS             complement(14730..>14832)
                     /locus_tag="ACHKNB_RS18075"
                     /old_locus_tag="pgaptmp_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4197058.1"
                     /translation="NATFRKELEKILRDPELSQALRDELSALVPEQV"
BASE COUNT         3667 a         4243 c         3365 g         3557 t
ORIGIN      
        1 ataaccccat tcagtcttat ccgaatcagc atactgtgag ctaaccgaat tacacccagt
       61 taacaatgcc agtaatggtg cgataaaaat gattttatct ctggtcatgt attgtcatcc
      121 tgtagcgtta aacaccagac atcataactt tagtattttt tactgcgtta tcattcagct
      181 attgcccgtt tttttacgcc agatgaaaat tagattttct ttttatatgc tcatcattat
      241 tccggtttga gtcaccagtt gccgtagggg taaattttcc ctttgataaa atctggtgta
      301 tcaccgtaat aaacatatcc atctggatgt ttagtaaccc ccttacacat cttcatttta
      361 tcacccgata ctgtagtaat tttagcatcc aactgaggta ccaccggatt accgcgatct
      421 cccagccaga ctcttacctt cccttcaggt gccagaccaa taatcatcgt tgaacgataa
      481 taggtttcgt tccagcggtt gtctgtgtaa ggcgtaacca tctgctccca ggtccctttt
      541 ttaaaaaaaa tcagagtttc ataactcttt ttatcaatta ttgaatccca gcaaaaacgc
      601 aacatcagag gtaatgcttt ccccttgtta taatacgact cagtcgcgcc cagatggtca
      661 ctccagcgcc ctacactgac ctgactcggc tcggtattat caagatactg ataagtcgtc
      721 ttataacctt ccccgtccac catcaatgcc tgagtggcca gcgcaggcag agcctttgga
      781 taaataaaga gaaatttcca ttcatcatag ggtaattttt tataacccca ttcagtctta
      841 tccgaatcag catactgtga gctaacagaa ttacacccag ttaacaatgc caataatggt
      901 gcgataaaaa tgattttatc tctggtcatg tattgtcatc ctgtagcgtt aaacaccaga
      961 catcataact ttagtatttt ttactgcgtt atcattcaat cgctgctgat catccatgta
     1021 ccatacagaa cgaatccagc gatcatttgg tttattcgga aaaacgaacg ccttattacc
     1081 tttaggacca aagcgatgga taaaaatctc tcccgttgta ggatccaccc ataaattacc
     1141 atcagactca atattccagt tggcagaaca atgggtgtat tttccgataa tatacaactc
     1201 ttcctcagta aaagcctgag gctctttccc caaacgtacc gcttttccct gagcaatggc
     1261 tttatcaaca aaaggaatta attcaggaga taaattcata tctggatttt tggggtcaat
     1321 tggagcaaac aatacccctg cttcctgagc agcatccagc atcacacgca ggcaaacatt
     1381 agcccagtca ttaggtactg ccatacgctc aaaaaatact gcggctccca cctgcttttg
     1441 tatcacgcca gcccgacgtt gattgctgtt gttcacacct acggaacgaa ttttggtggt
     1501 tagttttcca tgaggcaaaa tatgttttaa ggctggcaga gtcatcaaca tttttctggc
     1561 ttgctctgca ttacggtata ctcgcgtatc cgtttctctg gtatcgtcac tgacaatttc
     1621 aaatgccggg caactcagaa acagactttc attttcctgc agaggtgaac ctaccggatt
     1681 gtaaccacct ccaatatctg aatgcgcccc tggtaaagcc agttcgggcc acataccttt
     1741 aatactgttc aggctgaaat tatatcggta ttcattcatt gcagtaatct ggaaaacttt
     1801 cttcgcgatg gaaggacgca attccagctt tactcccggg ttgctacgtc cattaatatc
     1861 gaagaaatta ctgattccac caatagctgc gacagtatca aaaagcccca gaaaacggac
     1921 ttcacccgaa ggtttaccat cataaaaatc gcctctgagc cctttagcaa tagctctggc
     1981 tatagcagga tcctgttcca taacccgatt agcaaagtgc cttgccgccg ctgctccccg
     2041 gctaaaacca aaaacatcaa actggatttt tgcgatacaa aaatcaggat ttaaattgaa
     2101 ccccataaat tctgataacg cctgggtgat tcgttccatt gcctcgtcgg ttttcgtcac
     2161 cacaccttca aatatatcca gaatactggt gcctaatccc atgccaatga ctgaatctgc
     2221 ttcaccagcc gcagtcccta tcccggaaat gtaagtctta atttgagctg acgtctggtc
     2281 ttttttaagt acctgatctg gatgatagag tatattaagc cagtgaatat tggaataata
     2341 tccacgataa ctgccattcg agatattgtt cttgttaatt tcttcaagaa aatcctcaca
     2401 gctttgtgca tctttttgat ttaccccctg tctggctgca ctacattcat taaatttcat
     2461 cagacgagat gctgtattct cccgattgtt tccggtacca tcgaaaaata taccaatggt
     2521 taaagtcaca ccccgcgccg gttttttctt tgcaacaaca actttccagt cattatgaat
     2581 actggtttta tgggtggcat ctgttacgtc atatccctga tattttgtca ttctgtttct
     2641 ccatgaattt ttaccttccc aaactcaact tttactactg acggaatgga ggtgttgact
     2701 ggcgctgttt taccattttc atcggtgatc ccctgtagaa tatcaccttc agccgttgtt
     2761 aatcgataag gcgtcgaagg ttgcggcact cccgcttcat cggtaagaat aaaaccgcca
     2821 ccaaaccctc gcggcatttc aacagacgca atatcaagac ttgttggtcc catcttctgc
     2881 atattcgtgc tttttaacag gatctgtccc gggcatccca gctcaatatt tccaccgctt
     2941 agtttgatat aagctccacc acatgccagt atcagttcct gattagcaga aacagtcact
     3001 ttgccctcag aactttgaat atcaagattc tgttgggaac tcattgacaa atcgtcactt
     3061 tgagcctgaa tatttacccg cccttttgct gctaacagtt gcatcccctg gtttctgctc
     3121 aacaggctaa cagcaccttc tgctacaact gtaaatgact gcccggcact aatatcagtg
     3181 ttatctcccg acattatgcc cacgctttca ctacccgaag caattcgtaa cgcccgtgca
     3241 ctaaccatgc caatcccatc aggagcatgc aacagcattc ccggcgcagt gagatctgtt
     3301 agcgatgaat tcaaccgttg ctgacaactt atatctccct gagtagcatt tgcagcagtt
     3361 gcggccttcg ccatacttcg ggccagagat aatgcctgct ccagttggcg gatagcttca
     3421 ttcatatcca gcatctttcc ttgtgcctga ggctgcatat ctgcactgat aaatattcct
     3481 tttcctcccc ggatacttat ccagtcatca gtcctcagct cagcgccttc tcccctgagt
     3541 ttccgggagg cattaacatt atgaccaaga ttaagttgtg tcttaccgcc aaactcggta
     3601 ctgatcttga tatgctcctc accgcgtttg tcctccatcc gcaacttgtt atttgccggc
     3661 gtacggataa cattgcgcgt accgttctta tcggtaacgt gatcaacatg acgtgaatca
     3721 tgcagggcat gcgcgatata ggggcgatcc ggatcacctt cctcaaaggc aatagccact
     3781 tccgttccct gaatcagcgg aaaatgaaaa ccataagcat caccactgta gggtttggcc
     3841 agacgtaccg gcatgctttc ctgtcccgga tttttatcat cacggtcagc atcaaatttc
     3901 acccggtaca tgcctgatgc atcctgccag gcgtagatat ccccttcttt tgcactggtc
     3961 acacgggccg tcatggtacc ggtgattttc ggacgaggaa gtaacggtgg tcgccagcag
     4021 ataacttcac tgtaaggaac gcccttcagt acaacctgca gggcatcctt gcgacttgca
     4081 gaaaaaccga cacaggtaag caacaccgga ttgcgcagca gcgcaggcaa cgtcggcgga
     4141 agactgtcgg tgatagtcag aacctgagca ggcgccagcg tggcgtctgt gctgttgccg
     4201 gttatccgta cctgctccgc cagataacgt tcatgaccaa ggcgggcata aaaattggcc
     4261 gtctccggca ccgggtcgat tttatccccc cgttccagat gacggaaatg gtagtggtac
     4321 acctcaccat agttaattcc gtcaccatca ccatgcgtca tgtctgccgg ggcggactgc
     4381 aacagatatt gtgcttcacg atggttataa gcctttgtgg tgacactggc ctctgccaca
     4441 ttgtgcgtca cattcagccc ccacactgaa tccgctctgt tatcattcat tccggacgga
     4501 ctgtttatcg ccagcgtctt atcaaacgtc agcgcagcct ggctgtcacc aaaatgcact
     4561 atttccgtct gcgtgtccgg ttgcagggtg aaaaaataaa aaatccccac ttcagccagc
     4621 aaacgctgaa taaattgcag gtcactttcc tgatactggt taatttgttc acgcttcgga
     4681 tacgtttttt tgagacggaa ctcaaactcc cagcctttca ggccgtgctc ctggagaacc
     4741 tctgtcacca cctccggcac cgatttgtta acgaaaaatc ggtgagaacg atgctgcctg
     4801 tccagtagtt taataaatgg ttccagtatc acctgatatt ttgcctggtc ggcggagccc
     4861 gatatgcggc gaaaatctgt aatgacacca tgtactactt tctggttcac ctgcccctta
     4921 agccctcccg tacccattgt caatgtggct gatttacgca gcaacagccc ggcatccagg
     4981 tcgttgtcag tactggtaaa attgatgatg taataatagg tctgactcat ctcctctctg
     5041 ccgctgaaat gctcaacatc cagtggtgac gggcaggagg gaataattaa atggtaacga
     5101 ttcagggaag cagagatagt ttttgaaagt gtatctgtca gactcatatt gttaactcca
     5161 tgtctgtacc ggtattgaac tgcaattata taatgatggc cgttaagctt tattttcaga
     5221 gagaattact gataatcatc ccattcatcg taaatatatg catatgcaaa tcgatacatc
     5281 agataaccga caaaaaataa cgcaacagag agccctcccc acagatattt aaattcagca
     5341 gatgagagaa aaaagaacca gactgtaaat acaatacccg acgcacctaa ccccatacct
     5401 gcgccacgga taaatcggga aagcattata agtaatgttc tttttatctt cataaaccac
     5461 cgaaaaaata ttcgcatatc cggtcaaact cagccgaatt tcggtctgcg acaatacagg
     5521 ctcgttcaaa cgggcgcaca atatcttcaa caagaaagta caaaagatcc aaatccccct
     5581 gagctttcag cttatcatgc aacaccggat ttctgtcttt cagaaaacgg gatgtgtaaa
     5641 tcgcacgcga tgtctctgcg ccaacagata ataacaaccc ggcaaaactc cctgcaaaaa
     5701 aagcagccag ccctctggat accagaacct ctcccagttt aaccccggtc aattcagcaa
     5761 atgccattct acctgccaga tctcctgaaa cgttcttcac aatggagcct gttttttcca
     5821 tattgatccg ggatacaaag tcttcaagac aaatactgat aattttccca ataatatcac
     5881 ggctgaaaat gcctttttct accagtctgg ccattctgaa tttgtcattc tggttttccc
     5941 gcctatgccc ggtatctata aaatcatatc ccatataacc gagactgact ggcaaagata
     6001 taagtccctt cccgacggcc cccagaaacc catccccatt aatatttacg gcctccagca
     6061 tatcccgggc aatttcttta gcactgtcca tatatgcttt cctgcggata atcgctattg
     6121 attccatcaa acgctatcac aataccatcc tcatcatgcc acgtcagaga caggtacgtc
     6181 ggatgctgtc tggcagcaag atagctgagc aactgccggc tcagtaccgg cagcagctgc
     6241 tggttcagca gactctccac attccgggcg cccgtatccg gcagcaggca ggcggatgtc
     6301 agggcatcgt acacacgttc atcaaccgat gtgctcagcc cataatggca gtgcaggcgt
     6361 tcacagacct gtgcaagctt catctccaca atgatgcgca tggcagactg cgtcagcgga
     6421 cggtaaatca cggtccggaa acgggcaagc agtgctggct ggaagtggtc gcgcagcagt
     6481 ggacggataa gctcatgcag ctccgattcc gttgtctccg gcttttcgct cagttgctgc
     6541 atcagaaggt cactgcccag attggaggtc atcagaatga ccgtattacg gaagtcaatt
     6601 tcacgcccct caccgtcgcg cataaagccc cggtcaaaca cctggtagaa cagattcagc
     6661 acatcccggt gggccttttc cacttcatcc agcagcacca cactgtacgg acgcttacgt
     6721 acagcttccg tcagaatacc cccctgaccg tatcccacgt acccgggcgg tgaaccctta
     6781 agctgggaaa ccgtatgggg ttcctggtat tccgacagat tgatggtaat cagtgatttt
     6841 tcgccgccgt acatcacatc tgccagcgcc agtgccgttt cggtttttcc cacaccactg
     6901 ggaccgacca gcaggaacac gccctggggg ccgttaccgg atgtcagacc cgttttcgcc
     6961 gcacgcagac gctgcgcaat ggatcccagt gccacgtcct gtccgaccac ccgtctgccg
     7021 atatcctttt ccagatgcag cagttccgtc tgttcgtcct tcatcaggga agataacggc
     7081 accccggtcc agtccgcgac cacaccggca acggtgcgta cgtcaacatc cacactgagc
     7141 aggggctgat tctgctgaat gtcgtgcagt gcctgctgca gatgcagtgt ctcactctga
     7201 cgggaaatat cgctgcgact ttcaagtaac tcaccggtga gttcgcactc ccgggcgtac
     7261 tgctgctcca ggtcatcacg tgctgtcatc agcacattct cttcctgctc aatggcagac
     7321 agacgttcgc cgtgattctg atgaccaacg gcaatatctt ccagcagtgc ctgtttttcc
     7381 ataccgaggg aagcgagctg tgaacggata cgggtcagct gttccggcac cgtgtcgagg
     7441 ctcatacgca cacgggcagc agcagtatcg agcaggtcaa cggccttgtc cggtaactgg
     7501 cgttctgtca gataacggcg cgacagcgtg acggcagcac gtaccgcctc atcggtgata
     7561 tgcacgttat gatgttccgc ataacgggat ttcagggagc gcagcatcag acaggccgtc
     7621 tcatcatccg gctcgtccac tctgaccatc tgaaaacgcc gctccagagc cgcgtcacgt
     7681 tcaaagtatt gtttgtactc agaccaggtg gtggcggcaa tggtacgcag ttcaccccgc
     7741 gccagcgcag gcttcagcag gttggccgca tccgcgccgc cggcctgatt accggccccg
     7801 ataagggtat gggcttcatc tataaacaac agtacaggag ccggtgactg ctgcacggca
     7861 tcaatcacgt ttttcaggcg ctgttcaaat tcacccttca cgccggcacc ggcctgcagc
     7921 aggccgaggt caagggtacg cagggtgacg ggtctgagtg attccggtac gttaccttcc
     7981 gcaatacgca gggcgagccc ttccaccaga gccgttttcc ccacaccggg gtctcccacc
     8041 agaatagggt tgttcttgcg gcggcgggag agaatatcca ccatctgacg gatttccgta
     8101 tcgcgtcccg ataccgggtc aattttcccc tctctcgcgc tggccgtgat gtcccgggtg
     8161 aatttatcca gcgcactctg taacacaggc ggcaggtcac ctgacggtat acccgccgta
     8221 tccgcaccga cgggacgccc gataaaggtc acattgtctc cctgaggcag cacaggctct
     8281 gcatcctgta atctctccgg acactcatca gactgcgcat ccagcagcgg ggacagacgc
     8341 tgcaactggc tctgactcag tgtcagcagg ggccacaccc cgtcacagcg aaccaggtgc
     8401 ggattttccg tcagggcacc cagcagatgc acgctgcgga tatgttcttc cccctgtaat
     8461 gaggccacca tccaggcctg tttcagcagg gcattcaggg actgcgcaag ctgcgggcgg
     8521 ctgcgtacgc tacggggctg gttatccagc cagccgagca gcgactgcca tattgcgtcc
     8581 atatcccagt cataacgccg gcccagcacg gtaaggtctc cttccccctg ctccagcagt
     8641 ttcagtaacc agtgctccgg ggtgatttcc gcatgggccc gggtctggca aagggaggct
     8701 gcgccttcca gtgcacgggc acagtaatga ttaagacgac gtaacagggc tgccggattt
     8761 tccatgttat tcactcactg tcagaagacc ttcagacacg ctaccgccct tcgccataac
     8821 ccgggcgcat caggtctgtg ttcaggattg agaattcttt cttcagcacc cgccaacaga
     8881 tactgaagaa aaaagaaaat gacggacagg ataccctgcc cgtccgggag aattacgcag
     8941 tggtacgttc attccacgcg tcagaatgaa tgatgttacc gtccttataa gtccaggtga
     9001 ttttctcgta gcgcagctcc acctgctcaa ggtggttatg cttctcgtag gcgggattct
     9061 tgatgtcgtg catgaccggg ttaaccttca ccaccttcac gttctcaagc ttcgtattga
     9121 aatactccac ttcctgaccg gcatcattga ttctgtacca cttaaattca gcagacttca
     9181 gggtctgccc cgtggtcacc gccttgtaaa ggtaggggct ggaggaatca atttccttgg
     9241 taaacaggaa cggcgtgtga atacgggtac cggtcagctt accggtattg ttatccgtcg
     9301 gaatgtaaag gcagtgctcc tgagccacca cttcaatact gccctcacgg tccttcacat
     9361 ccacggagcc tttaatatcc gctccgccat catcctgcag ccacagataa acaggaattg
     9421 ccataaagtt actccctctc gttgtcttac gcgccctgtt acggcgcccg ggtgatgtcc
     9481 ggcagacgac aggcatcggc ctgcggtacc agactgattt caacacggcg gttacgcgct
     9541 cgcccttccg ccgtctcatt cggagccacg ggacgactct caccatatcc ctgtacggca
     9601 aaacagcttt gagggatatc gcccgtatcc cgcatccagt cgcgaacggc ctctgcgcgc
     9661 ttcagtgaca gggcctggtt gcgctgcggg tcgcccgtat tgtccgtgtg accactgacc
     9721 acaatcagcc agcccgcttt tgccttaata tccaccaggg cattcaccag ccattttgtg
     9781 gcgcccggtt tcagcgtcca tttaccggca tcaaacagcg ccaggctgtt aaggcgaacc
     9841 attttcggcg tctgagtaac tttctgtatt accggacggg gaggcagtgg tgcaatccac
     9901 tcaccagtcg ctcgctcaac cggcggtatc agccacattc cctgatacaa tcccagtccg
     9961 taacgctgcg gcacaccacg ttgctgccag tcgttcagta actggctgtc agcctgaaga
    10021 cgttgcagtg cagttttttt cgattcagat gaatatccgc gcatccggtg ccaggccgtc
    10081 aggtgttccc ccacgctgcg aaccagtcgc tggttattca taaaagacgc cagcatagcc
    10141 agcagaagaa aaacgccgac aatcgccccc gtctggagac aaagtctgtg ccagtgactg
    10201 agtccacgat gacggggaat accggacagc agaatatccg gcaacggcag tgcctgtgat
    10261 gacacgccgg caggcggtgg cagtacagtg agtgacgtca gttgttgttg ccagagatta
    10321 ccgggccggg ccgcaaccga cgtcagacag aatccaacgg caaccggccg gaaataaaag
    10381 tgctcctgcc ggcaaatatc tgtcagggca tcagtaaccc ggttgtacca ggacaacatg
    10441 gcattcagcc ataacagttc actgacgcac cggagcctgg acgctgcatc acactcccgg
    10501 agccattcct ctgccggaat ggctccggct tccgtcaggc gtatgtgcat tcccgacagg
    10561 tccggtgtca gtgtaaacca gtgtgcagag tgcttattta aggatgtgtt ttgtggtgac
    10621 ggccctgaaa ccatcaatgt caccagcacc ggcggcatgg tattcagcca ctgccggcac
    10681 tgcatgactg cacgctgcca gttccgcaac cacgacagga aagcttcatc tgaggtatgc
    10741 tgctccggta aaagtcccag catgaccacg acctggccac acagggccgg acggctgtac
    10801 gacaaccgct ctgccagtat tctcatctgc tccggcgtat ccacacgaag ataccatccc
    10861 gatgctgttt cccggtatgc ctgaccgggt tcgaaccacg catctgagtt tccggcaaca
    10921 agcaacacat gcccctgaaa atactcaggc ggcagaccgt catcgccggc attctccggg
    10981 atatcacgac gggaagacag acaacgccac tgataccaca gcacaagccc cgccgccaca
    11041 actgaaacca gggtcagtat tatctggttt actgcagaca gtggcaggaa tcccgaaatc
    11101 agccacagtg tcaggaacag actcagtatt gtcagccaga gacgggaacg tacgtgcatg
    11161 gttatccctg cccggtaagc cgtgccacct ggtctgcgag cacagaagaa agcacacacc
    11221 agagaccagc cagcgtcaca atacccgcca cccatgacaa ccagtacatc gttctgccaa
    11281 cacggtaacc cggtgcacgc accaccaccg gcgcatcctg tgcgaaagta aatgcaggaa
    11341 cacgcgctgt cagtgcgcga attacatctt cgcgacgctc atcatcctgc gaccggtact
    11401 gaccggtaaa accgagctga agcgtacggc agaggcatgt cagtaccgcc acatccggcg
    11461 caggtaattt cagttgctca cgaatacgct cccagagctc ttctccggca ttgagcgtac
    11521 caaaaaaatg agcctgcagc gggtcctgct gccaggtgtg ccagccatcg tctgtttttt
    11581 cacggttcag cacactctcg tccagcaggg cgcagaaggc atacaacatg atttcactgc
    11641 tttcctgact gaattctgct tcagccagct cttcacgcgc ctgcgtcacc agctgacagg
    11701 cacggcgata cagggcctta ccatcctcaa ccggttgtcc gcagcgcagc tggctgacca
    11761 tcagccagcc gggataaaaa atctgttcag cccgggagat aacaggttta ttcatgtccg
    11821 cagtaccgca aaaagttcca gtttcacatc ccccagcgat gccggggtgt agaaagtaca
    11881 acgccccgcg tccagcatcg cccgggctgc atccgtactt aaatccagcg cgaaatactg
    11941 gttttccagc cggagcggaa tggccgccgg tacatgcgta accggacgga taatgacccc
    12001 gctcagcgcc acattcacca cctcagacac atcatcgggg ctcccggctt tgcataactg
    12061 cggaaacttc gtctgcagtt catgcccggg tatggaagag cgcactgaaa gccagaaatc
    12121 cgccccttcg cgcaaacgcg catcatgtaa tgccccctcc cacatcacac cattttgttt
    12181 cagttcaatg aaaaccacac gggaaggcag gcttgcctcc agcagctggt tcagcaaaga
    12241 aagcaagggt ggaaacacat tttcaggtgt ttcatgacgg tatgccggaa ccgcatccac
    12301 attatgctca agcgagaatg tgagcaggga gcctgccagc cgggcaagct ccctgtacaa
    12361 cagttccggg tggcggtaag gcatgtcaag caactctttc agaacaggct ccgcactgtt
    12421 cagggcattc agcaaccaga acaacgacac gtccgccact gcaaaatccg caagacgggc
    12481 attgttctcc cgacgcatgg acatcagtcg ctggcgacgc gcctgcaggt gataaagcaa
    12541 ctccgcaagc tcagtcatca gcgaaggact ggcagacagc gtcagcagag ggggaataaa
    12601 acgcgggtcc ctgcaccact gtccctgtgc atctcgaaca agacgggtaa caggacaggt
    12661 cagccaggca gcattttcct gatgagccat gcgcagagtc aggttatgcc gcaataccgc
    12721 cacttcgctt tgctcatgcc cggccagctc ctgcacattc accctttctg atttccagcg
    12781 acgcggacgc tcgctctcac tgccatcatc aaggttgcca ccgttagcat tcagtaacgg
    12841 tagcgccagc acaatatcca ccagggaacg ctcagaaacc gtcgataaat cacagaccgg
    12901 aggcagatta tccgcacgtt ccgtatcaat aagcgttccg tccgggaaac gaacaatcag
    12961 acgggtggca ttcaaccgtg aaagcggtaa cagagagtca tcaaactctg cagccaccac
    13021 gccccaggga tgcgccagcc ccattcgggc aacgctgtct gccagatgaa catcccaggc
    13081 agcctgctgc tgaaactgct ggggcatcag aaaagccccg tcttcccaca gtggacgata
    13141 aatcttcatt tcccgacgcc tgccttacgc ttttgccttc ggcatctgcg aaaccagtga
    13201 cagactgacg tccatacctt ccacctggaa atgtggtaca gcatagagtt tcacgcggaa
    13261 aaatcccggg ttgtcttcga tgtcttccac caccactttt ccgtcacgca gcggatgtga
    13321 ggcctgcagt tcatcacccg gatcggtcat ctccgtcacc agaccccaga tccagttatt
    13381 cagttccagt tcaagcagac ggcgatcttt ggtggtaccg atattctccc tctgaatgat
    13441 cttcaggtaa tgcgcaatgc gggacagcag gaagatatag ggaagacggg cattaatacg
    13501 gctgttcgcc gttgcatccg gcgtatcgta cagtgccggt ttctgggtgg agttagcgga
    13561 gaagaagcag gcataatccc ggttcttgta gtaagagagc ggaataaagc ccagattagc
    13621 gaattcaaac tcccgagttt ccggaatcat cacctctgac ggaattttga cctgattacc
    13681 ggttcccaga tcatacagat ggatcggcaa atcctgcacc gcaccgccgg cctgaggacc
    13741 gcggatctga acacaccagc cgttggtgac aaaactgcgc accatattgg ccgcaaaagc
    13801 aaaagacgca tttgtccaga gatacttatg atgatccgga cctttcacct cttcaacata
    13861 gttaaagctg cggacaggta cggtatcagg accatatggc aggcgtccca gaacacgtgg
    13921 catcaccaga ccaatatagc gggcatcatc cgtttcacgg aaagcattcc acttgatgta
    13981 ctcggcacgc tcaaagtgat tgccaatatc cttaatggcg gccacctctt ccatcgtatc
    14041 cttccggaag aaagccgggc caactgaacc aataaatggc atatgcgcgg acgcagccac
    14101 ccgggaaata tttttcagca gcgccacatc ctgcgcagac gcatcaaact cccaggcaga
    14161 aatcagggct gcaatgggtt caccacccgg cgtatcgtac tccgccacat aagtctgcag
    14221 ataaagacca ctctgaataa tttccggcgc atcctcaaaa tcctgacgca gatcgtcttt
    14281 ggacagatcc agcagttcaa ttttcacatt acggcggaag tccgttttat ccaccagaga
    14341 cttcacgccc cgccacaggg actccacttt ctgaaattcc ggatgatgaa gcacggcatc
    14401 cagctgacgg ctgatctgat aatcaagctc tgcaatatga tggtcaatca gcgccttatc
    14461 gagtttctcc accggtgctc ccgctttcgc caggcattcc ataaacacct gcatccctgc
    14521 ggtcagacgt tcatctgctg atgcctctga catggcctgc ggatcctgcc agatatccag
    14581 cgcactcagg gctgataccg gattcagatt aattttttca aacagggaag catatacgct
    14641 gtcgccggaa tgcgtttcag aaaccgcatt tcccgccaca ttattttctt tctgtacaga
    14701 catcagcatg ctctctctta atccgttaat cacacctgtt ccggcaccag tgcactcagt
    14761 tcgtcacgta aggcctgact cagttcggga tcccgcagaa ttttttccag ttctttacgg
    14821 aaagtggcgt ta
//
LOCUS       DAZFVO020000125        14663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFVO020000125 DAZFVO020000000
VERSION     DAZFVO020000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.126"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..167)
                     /locus_tag="ACHKNB_RS18080"
                     /old_locus_tag="pgaptmp_003616"
     CDS             complement(<1..167)
                     /locus_tag="ACHKNB_RS18080"
                     /old_locus_tag="pgaptmp_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HGU4197059.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTP"
     gene            complement(171..530)
                     /gene="yeaR"
                     /locus_tag="ACHKNB_RS18085"
                     /old_locus_tag="pgaptmp_003617"
     CDS             complement(171..530)
                     /gene="yeaR"
                     /locus_tag="ACHKNB_RS18085"
                     /old_locus_tag="pgaptmp_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HGU4197060.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(703..1341)
                     /gene="leuE"
                     /locus_tag="ACHKNB_RS18090"
                     /old_locus_tag="pgaptmp_003618"
     CDS             complement(703..1341)
                     /gene="leuE"
                     /locus_tag="ACHKNB_RS18090"
                     /old_locus_tag="pgaptmp_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HGU4197061.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1468..2391)
                     /gene="dmlR"
                     /locus_tag="ACHKNB_RS18095"
                     /old_locus_tag="pgaptmp_003619"
     CDS             complement(1468..2391)
                     /gene="dmlR"
                     /locus_tag="ACHKNB_RS18095"
                     /old_locus_tag="pgaptmp_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HGU4197062.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2494..3579
                     /gene="dmlA"
                     /locus_tag="ACHKNB_RS18100"
                     /old_locus_tag="pgaptmp_003620"
     CDS             2494..3579
                     /gene="dmlA"
                     /locus_tag="ACHKNB_RS18100"
                     /old_locus_tag="pgaptmp_003620"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HGU4197063.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASSEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3830..5440
                     /gene="yeaV"
                     /locus_tag="ACHKNB_RS18105"
                     /old_locus_tag="pgaptmp_003621"
     CDS             3830..5440
                     /gene="yeaV"
                     /locus_tag="ACHKNB_RS18105"
                     /old_locus_tag="pgaptmp_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HGU4197064.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5472..6596
                     /gene="yeaW"
                     /locus_tag="ACHKNB_RS18110"
                     /old_locus_tag="pgaptmp_003622"
     CDS             5472..6596
                     /gene="yeaW"
                     /locus_tag="ACHKNB_RS18110"
                     /old_locus_tag="pgaptmp_003622"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HGU4197065.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6652..7617
                     /gene="yeaX"
                     /locus_tag="ACHKNB_RS18115"
                     /old_locus_tag="pgaptmp_003623"
     CDS             6652..7617
                     /gene="yeaX"
                     /locus_tag="ACHKNB_RS18115"
                     /old_locus_tag="pgaptmp_003623"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HGU4197066.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7671..8786)
                     /gene="rnd"
                     /locus_tag="ACHKNB_RS18120"
                     /old_locus_tag="pgaptmp_003624"
     CDS             complement(7671..8786)
                     /gene="rnd"
                     /locus_tag="ACHKNB_RS18120"
                     /old_locus_tag="pgaptmp_003624"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HGU4197067.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8868..10553)
                     /gene="fadD"
                     /locus_tag="ACHKNB_RS18125"
                     /old_locus_tag="pgaptmp_003625"
     CDS             complement(8868..10553)
                     /gene="fadD"
                     /locus_tag="ACHKNB_RS18125"
                     /old_locus_tag="pgaptmp_003625"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HGU4197068.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10758..11339)
                     /gene="yeaY"
                     /locus_tag="ACHKNB_RS18130"
                     /old_locus_tag="pgaptmp_003626"
     CDS             complement(10758..11339)
                     /gene="yeaY"
                     /locus_tag="ACHKNB_RS18130"
                     /old_locus_tag="pgaptmp_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HGU4197069.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11379..12074)
                     /gene="tsaB"
                     /locus_tag="ACHKNB_RS18135"
                     /old_locus_tag="pgaptmp_003627"
     CDS             complement(11379..12074)
                     /gene="tsaB"
                     /locus_tag="ACHKNB_RS18135"
                     /old_locus_tag="pgaptmp_003627"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGU4197070.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12132..14042)
                     /gene="yoaA"
                     /locus_tag="ACHKNB_RS18140"
                     /old_locus_tag="pgaptmp_003628"
     CDS             complement(12132..14042)
                     /gene="yoaA"
                     /locus_tag="ACHKNB_RS18140"
                     /old_locus_tag="pgaptmp_003628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HGU4197071.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14174..14518
                     /gene="yoaB"
                     /locus_tag="ACHKNB_RS18145"
                     /old_locus_tag="pgaptmp_003629"
     CDS             14174..14518
                     /gene="yoaB"
                     /locus_tag="ACHKNB_RS18145"
                     /old_locus_tag="pgaptmp_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGU4197072.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3549 a         3883 c         3625 g         3606 t
ORIGIN      
        1 ggggttttac ggcgcaccgg ataagaacgc acggtattca ccagatcttt tatcacttca
       61 gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc gacagaaacg
      121 ccattgctgt tattggtgac ggtcacggta tacgttgctt tgcccatgat tcatttcccg
      181 ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc cacgaagaag
      241 tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt ttctggaggg
      301 aacaccgcaa actgccccgc ttcgataagg atcacctgat caggctctgc actgtgttca
      361 tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg gtaaaccccc
      421 gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt ttgtttattc
      481 cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat cctattccct
      541 ccttcgggga atgcgctgaa aacggtttat tccagccgtt tcagggtacg cctgataatt
      601 tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa aaatcaggga
      661 ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt gcagcgtcgc
      721 cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa ctttagccag
      781 tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca ggaagctcaa
      841 atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa ttcccgtatg
      901 tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa tggctttcgg
      961 attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg gctcatcgga
     1021 tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc ccagatagag
     1081 caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg tcttaattaa
     1141 tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa atacaccgca
     1201 ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta caaacagggt
     1261 atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc agtaattcag
     1321 aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat gcgcgattaa
     1381 aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg ttttaacccg
     1441 atatcaaccc gataattgaa tcattaatca ggcatgctcc agtgaaaaat tcgggtagtg
     1501 ctcggcaaaa tactggcgta aaaattctac cgttatccgc actttcgctg acgtcgccag
     1561 ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta gcacttgcac
     1621 taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag caatcccctg
     1681 cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg ggccggtgac
     1741 tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag gatgatcgcg
     1801 ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg gcgcgccatg
     1861 ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca gtttgcgggc
     1921 gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac cttcattgac
     1981 caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat acgccttagc
     2041 cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc tgataatacg
     2101 cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct gaccgacatc
     2161 ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa tggtgacgcg
     2221 gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg cgatgcgctt
     2281 gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac cagcccgacg
     2341 agccaccagc ataaagacgc gcaaatcatt cagcagcggt aaattattca tgattcgtgt
     2401 tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg atggtgatgt
     2461 tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg tattgctgcg
     2521 atcccgggag acgggattgg caaagaagtc cttcctgaag ggattcgcgt gttacaggct
     2581 gccgctgagc gctggggctt cgccttgagt tttgagcaaa tggagtgggc gagcagcgag
     2641 tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact tagccgtttc
     2701 gacgccatct attttggtgc cgtcggctgg ccggacaccg ttcctgacca tatttcgttg
     2761 tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct gcgcccggtt
     2821 cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga catcgatttt
     2881 tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag agtgaatgaa
     2941 ggtacagagc atgaagtcgt cattcaggaa tcggtattta cgcgtcgtgg tgtcgatcgc
     3001 attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct aacttctgcc
     3061 actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt ggaagcaatg
     3121 gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct ctgcgcgcgt
     3181 tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttatt tggcgatatc
     3241 ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc cgccaacctg
     3301 aatccggaac gcactttccc gtcgctcttc gagccagtcc acggttccgc gccggatatc
     3361 tacgggaaaa acattgctaa ccctatcgcc acgatttggg ccggtgcaat gatgctcgat
     3421 tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct ggcagcaatt
     3481 gaagaagtga ttgctcacgg gccgaaaaca cctgatatga aaggcaatgc caccacgcca
     3541 caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag tttatttgaa
     3601 ccgcgtcact gacgcggttt ttttattcgt tctttgcagt aaataacctg cgtcatttca
     3661 ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg tttaatttat
     3721 gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc tgcaaataat
     3781 tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca tgatgagcaa
     3841 tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc ttttcatcgc
     3901 agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacgcaa tatacacttt
     3961 tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa tgggactggt
     4021 gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa aaccggaata
     4081 cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt ctacgcttta
     4141 ttggggggtt gctgaatggg cctattatta tcaaacgcct ggattaaata tcgcaccgcg
     4201 ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg gcatcagcgc
     4261 ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg tgcgtaaaaa
     4321 caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc cgcaaggccc
     4381 atggggaaaa ctggttgatt tgatgttcct gatcgccact gtcggcgcac tgactatttc
     4441 ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg gtttacccga
     4501 taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt gcctaagctc
     4561 gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct ggggcgcgtt
     4621 cctgctgcca ttactggtgc tgattgtcgg cccaaccgaa tttattacca acagcatcat
     4681 caatgccatc ggtctgacca cgcaaaactt cctgcaaatg agcttattca ccgatccgct
     4741 tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt ggatctcata
     4801 cacccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga ttaaagaagt
     4861 tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg gcgtaatgga
     4921 aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg tgctggaaac
     4981 actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg gtaaattgtt
     5041 cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg atgcggtggc
     5101 ctacaccatg gctgcgacca gtacgcgtaa tctccaggaa ggtgacgatc ctgaccgtgg
     5161 gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct tgtttaccgg
     5221 tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct tcctcgtcat
     5281 tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattacg ccgacattcc
     5341 ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg aaaaaacacc
     5401 agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat cctaacgata
     5461 aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat ttttgcgcta
     5521 acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac gcgtttgaac
     5581 acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc agcgaactgg
     5641 cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg ctggtacgcg
     5701 gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt catcagttgt
     5761 tgagcggtga aggaaaagca aaaaatgtga ttacctgccc gtatcacgca tgggcattca
     5821 aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat ttcgatagcg
     5881 acaaagcgca actggttccg gtgcgtctgg aagaatatgc cggattcgtc ttcatcaaca
     5941 tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg aaagtgctgg
     6001 aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc cgcacgcctg
     6061 ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt ccggcgcatc
     6121 caggtttctc cgactccgta caggttgatc gttactggca caccatgcac ggtaactgga
     6181 cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa gagggtacgg
     6241 atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc accccgatca
     6301 aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc ctgcaaaact
     6361 acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg attgagtggt
     6421 atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag aaagggctga
     6481 aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt agtggcattt
     6541 ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag gactaatgac
     6601 atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc aatgtcagac
     6661 tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca ggtgaaacgc
     6721 ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg aagtcacatc
     6781 attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact actgagttcg
     6841 ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc gcgcggcggt
     6901 tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc aacgcctaat
     6961 aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg cggtattggt
     7021 atcacccctt tcctgtcgca catggcagag ctgcaacaca gcgacgtcga ctggcagcta
     7081 cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt ccagcatccg
     7141 caggctgaga aagtccattt gcatcattca tcaaccggaa cacgactgga attagcgcga
     7201 ttattggcgg atatcgaacc tggcacacac gtttatacct gtggccccga ggcgctaatt
     7261 gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgccg ccgatacgct gcactttgag
     7321 caatttgcta tcgaagacaa aaccggcgat gcatttaccc tggtgcttgc ccgttccgga
     7381 aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa taataaagcc
     7441 gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac agcaatactg
     7501 gaaggtgaag ctgaccatcg ggatcaatat tttagcgatg aagagcgtgc cagccagcaa
     7561 agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga tttgtagttt
     7621 gccgaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt ttactgcgga
     7681 tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca gccggaaatc
     7741 agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag ttggttgatt
     7801 tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac gtcagtaatc
     7861 agcgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag gttaagcatc
     7921 ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc cagcagcgtt
     7981 ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc gcctaaactt
     8041 cccggcatat aacgcgctac cgaccacaaa tgctcttcac gcacgacaaa gttcaccgcc
     8101 agatcgcgct ctcgcgcctt gcgcagtcgc cagtcggcta acagttgcag acaggccagt
     8161 tggcgtgtgc gtaattgcca ggcattggtg atatcacgcc aggcatcttc cggcgcaacg
     8221 acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg tagccagccg
     8281 gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca gacatccgcc
     8341 gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt gcgcgattca
     8401 ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc ccatgacatc
     8461 gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca agggttgtgg taattcgcca
     8521 aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt tgtgatggac
     8581 ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg atcaattagc
     8641 gccagatgct cgccatcgaa aagttgaatc aaccccagct gcgggtaata agtgcgcgta
     8701 cgaacaaatt cagtatccag ggctatcgcc ggaaaggcac ggacggcttc acacaaagaa
     8761 gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgttttctct ttagtgggcg
     8821 tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacactca ggctttattg
     8881 tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc gacgttagat
     8941 ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt gagctgacgg
     9001 cggcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac tacgaagatt
     9061 ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc ctgtacgcca
     9121 ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc ggaaaccaga
     9181 atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat caccgcaatg
     9241 tcgccggtgt gtaaccagcc atttttgatg atctcatctg tagcatccgg acgctgccag
     9301 taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc cggtggtact
     9361 tcattatcat catcatccac cagtttggct tccgtcgacg gcaccggcaa accgatgcta
     9421 ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca ctcggtaagg
     9481 ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc caccacttgc
     9541 tgcactggca tccctccgcc tgcggaaaga tgcagactgg agaaatccag ctgctggaac
     9601 tctttattgt tcagcaacgc attgaacaag gtgttaacgc ccgtgatagc ggtaaacgga
     9661 tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat aagcaggttc
     9721 tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat gtgatacagc
     9781 ggcagcgccg tcaccaccag ctctttgccc ggatgcaaca gcggaccata ggtggcgttg
     9841 acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt cgccacacct
     9901 gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc gggtttgacg
     9961 tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc tggcagatgg
    10021 tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt gccttttgcc
    10081 gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt tttatcaacc
    10141 actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc gccgctatcg
    10201 ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac gacgatcatc
    10261 ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg catcatcaac
    10321 gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc aaacgcgcga
    10381 ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa cgcaggctga
    10441 tcggcgtagc gcgcgaccga ctgctcaaac atatctatca gagattgata acggtcaggg
    10501 ttgatctccg tcggaacgtc cgcgggataa cggttaagcc aaaccttctt caattcttca
    10561 cctctaaaat gcgtgttcgt cgtcatcgca accccaaatg atatacattc cgttaacaca
    10621 atattaactc atcataccag cttgataatt acccaacgaa aaggttgcga agcgcgtcac
    10681 tatttatttt tatctttacc gtaagaatgc agaaacagcg gaccagccgc tgtttctttt
    10741 tctttaaaaa caagcaatta ttcagttaca actgtttgta ctctcgcggg gccgggatta
    10801 taccagcccc atccgccata gccatagggc cagccacgac cgccataaaa ccatggatca
    10861 atcggctgag gcggcataat cacctgctgg gttaaatgcc aacgtttgta acccgttact
    10921 tgcatcacca taaatttata gggcgtgttg ccgatttttc cgtcaaccgc accagtgatt
    10981 ggcccgacta ccgtaaccag ttgtccacgg aaatccaccg ggtccagaaa accgttcata
    11041 tcggcataaa tgcgaccgcg agaaggttct cccagcgtcg gtctggctcc gctgtccagc
    11101 ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccgc aaccactttg
    11161 ccaccaaagc gtgcctcctg accaacgtac agctgcggcg cactcatcac ccgaactaaa
    11221 tcttgttgcg gcgtgggact gctgccttta atggcgtccg gcacagtgac acaaccgctc
    11281 agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg aaccgccatg
    11341 gtgcgactcc tttttttctc agggcatact cttaagattc attctttgcc cggaagtttc
    11401 ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc caccgtttta
    11461 ccctcggcaa acatctgaca cgcaatcggc agcatatctt cagcagcagg cagtaacact
    11521 tcgccatcgc gcaaaaccag cccgctctct ttaccgagat ccggccaggc ttgccagccc
    11581 gtccctaccg tcacccattc gccggaaagc tgttgcattc gttcatggac gatttcgggt
    11641 ttgagtacgg cttcggtttc ttcaccgtgc cagataccgt tttcatcacg ctgatattcg
    11701 gcccagtaaa cttcgcccat tcgcgcgtca atggctgaca gcacgcgggt tgcgccgttt
    11761 ttgcgccacg ccccttgcgc catcgtcatt agcgtggaga cgccaatcat cggtaattcc
    11821 gcgccaagcg ccagcccttg cgcgatgcca ataccaatgc gcacgccggt aaagctaccg
    11881 gggccgcgcc cgtaagccag agcgtttatg tcagtcaggg aagttccgct ggtggtcagg
    11941 atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag ctcaaaatga
    12001 gcgttgacag taccgtcgtt ccacagggcg acagagcagg cctctgtcgc ggtatcgata
    12061 gccagaattc gcatgggtct tcgtgctttt ttcaataaaa aggcgcgcat cataccatac
    12121 tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg ggcaatgtca
    12181 cgggtgcgcg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg gcgcatcacc
    12241 agccgattgt cacaaatcac caaaaccccg cgatcgtcgg cgtcgcgaat cagtcgccct
    12301 accccctgct tgagagtaat caccgcatcc ggcagttgta cttcatcgaa cgggtcgcca
    12361 ccgcgcaaac ggcaatcttc catgcgcgct tttaacagcg ggtcatccgg tgaggtaaac
    12421 ggcaacttgt cgataatcac caatgacaat gtatcgccac gcacgtccac cccttcccag
    12481 aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg caacagttgc
    12541 cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg gaactgctcg
    12601 gccagatcac gcatcatggc gtgcgaggta caaagcataa aacaacgacc gttgttagct
    12661 tcgatgatcg gtcgcagcat tgccgctaac tggcgagcag aacctggctg gttggtttgc
    12721 ggcagattgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg gcttggcaat
    12781 agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag atcgtcgttc
    12841 accgacagcg ttgctgaggt gaagatccag ctaccaggct tttgcgccat taactctttg
    12901 aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga agtgcattcg
    12961 taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg tgtgcgatac
    13021 aacgtagcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga cagtttcgcc
    13081 acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg aatttgcgga
    13141 ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg cagacgaaaa
    13201 tcctgcgcac tctgggcaag acgatcagcg cacttttgta actgctgggt gtcttttaat
    13261 tcggtgcggt aagcgatggt gatgtctttt gccaggtcga gcagttgtcg actggagagt
    13321 gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc gaagatcatg
    13381 acgtccgctt ccgggatcag ttcgccaaat ccactctctt taaccaccat atccgccaga
    13441 aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg tgctttgacc
    13501 acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt ggtgctggtg
    13561 accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat atcaccatcg
    13621 actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg taccggcaga
    13681 tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt tgagcgccct
    13741 ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg caaatcgcgg
    13801 ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt ctttttcgcc
    13861 cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc ttccaccacc
    13921 agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg ctgtggttct
    13981 cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa atcgtccgtc
    14041 acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct ttcgccagtt
    14101 gaagtggtga cggcgacctc acattgtggc agtctttgca gcacgaaaat ggaaggataa
    14161 tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt ctgatgtggt
    14221 aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg ccgatgcctt
    14281 tgagcaaacc gccaacacgc tggcgcagat tgatgccgtg ctggaaaaac agggcagcaa
    14341 taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact tcgcggcgat
    14401 gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccggtgcgct gcacggtaca
    14461 agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg cggtgtaagc
    14521 tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg gttttacata
    14581 aatcgaccag agattaagat attacctgtt gatgctcctg ctaattcatt atttattttt
    14641 ataatttcaa ttttatctat aaa
//
LOCUS       DAZFVO020000126        14607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFVO020000126 DAZFVO020000000
VERSION     DAZFVO020000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.127"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..90)
                     /locus_tag="ACHKNB_RS18150"
                     /old_locus_tag="pgaptmp_003630"
     CDS             complement(<1..90)
                     /locus_tag="ACHKNB_RS18150"
                     /old_locus_tag="pgaptmp_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGU4197073.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLA"
     gene            complement(389..1771)
                     /gene="yahJ"
                     /locus_tag="ACHKNB_RS18155"
                     /old_locus_tag="pgaptmp_003631"
     CDS             complement(389..1771)
                     /gene="yahJ"
                     /locus_tag="ACHKNB_RS18155"
                     /old_locus_tag="pgaptmp_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HGU4197074.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYDGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(1781..2731)
                     /gene="yahI"
                     /locus_tag="ACHKNB_RS18160"
                     /old_locus_tag="pgaptmp_003632"
     CDS             complement(1781..2731)
                     /gene="yahI"
                     /locus_tag="ACHKNB_RS18160"
                     /old_locus_tag="pgaptmp_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HGU4197075.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKAL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(2874..4292)
                     /gene="yahG"
                     /locus_tag="ACHKNB_RS18165"
                     /old_locus_tag="pgaptmp_003633"
     CDS             complement(2874..4292)
                     /gene="yahG"
                     /locus_tag="ACHKNB_RS18165"
                     /old_locus_tag="pgaptmp_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4197076.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4292..5839)
                     /gene="fdrA"
                     /locus_tag="ACHKNB_RS18170"
                     /old_locus_tag="pgaptmp_003634"
     CDS             complement(4292..5839)
                     /gene="fdrA"
                     /locus_tag="ACHKNB_RS18170"
                     /old_locus_tag="pgaptmp_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4197077.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(5829..6692)
                     /gene="yahE"
                     /locus_tag="ACHKNB_RS18175"
                     /old_locus_tag="pgaptmp_003635"
     CDS             complement(5829..6692)
                     /gene="yahE"
                     /locus_tag="ACHKNB_RS18175"
                     /old_locus_tag="pgaptmp_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4197078.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(6732..7337)
                     /gene="yahD"
                     /locus_tag="ACHKNB_RS18180"
                     /old_locus_tag="pgaptmp_003636"
     CDS             complement(6732..7337)
                     /gene="yahD"
                     /locus_tag="ACHKNB_RS18180"
                     /old_locus_tag="pgaptmp_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HGU4197079.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            7595..8092
                     /gene="yahC"
                     /locus_tag="ACHKNB_RS18185"
                     /old_locus_tag="pgaptmp_003637"
     CDS             7595..8092
                     /gene="yahC"
                     /locus_tag="ACHKNB_RS18185"
                     /old_locus_tag="pgaptmp_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HGU4197080.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8184..9116
                     /gene="yahB"
                     /locus_tag="ACHKNB_RS18190"
                     /old_locus_tag="pgaptmp_003638"
     CDS             8184..9116
                     /gene="yahB"
                     /locus_tag="ACHKNB_RS18190"
                     /old_locus_tag="pgaptmp_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4197081.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9158..10246)
                     /gene="pdeL"
                     /locus_tag="ACHKNB_RS18195"
                     /old_locus_tag="pgaptmp_003639"
     CDS             complement(9158..10246)
                     /gene="pdeL"
                     /locus_tag="ACHKNB_RS18195"
                     /old_locus_tag="pgaptmp_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4197082.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKLGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10388..14371)
                     /gene="ehaA"
                     /locus_tag="ACHKNB_RS18200"
                     /old_locus_tag="pgaptmp_003640"
     CDS             complement(10388..14371)
                     /gene="ehaA"
                     /locus_tag="ACHKNB_RS18200"
                     /old_locus_tag="pgaptmp_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HGU4197083.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3354 a         3971 c         3446 g         3836 t
ORIGIN      
        1 tgcgaggtga tcggccaacg ctgctgaatc gccaaaagta caaaaggaag catggggata
       61 tttttgtgag aatttttctt ttatggtcat ggcaatattt ttgcgattac ggaaaaacaa
      121 atcacatctt aattactgtt ggtaaccatc agcaagagta tcattggttt ttcgatactg
      181 aatctttttt atccgaagca ggtcacattt atggcctgat aaataccttt gttaaaactc
      241 acttttgtgt atttctctca aataatcccc tggaaattat gccgcgagat tataaagttc
      301 aggatcattc attagcaaac cattcccgcc ccactccctg gggcttaaat ctcttacaat
      361 tcaccgttgc aggcgtttca aatgcgcatc aacctgccac actcccccac accagttgcc
      421 ctttatggaa cgttgcggtt ctcggcgaga tacgcgccac cgcctcggcg gaacaggagg
      481 cgtccaccag cacaaagctg gcatcatcct gcgcttttgg ccatacacgc tcgccttttt
      541 cattcagcgg caatatatcg ccagtggcta gaaacagcga acgggagagg ttctgttcgt
      601 taggacgaat atagagctgc gcgtagagat tggctttttc cagcatgtcg cccagaccat
      661 aaggcgacca gtggtcgata acgctgtcag tgccagtcat cacttttacg cctttgtcgt
      721 gcaactgttt gagcggcata tgcagcgtgc caatcggcac cgtcgaggcg atagaaattt
      781 gctgcgccgc catccgattc gccagcgcat cgacctgttg ttcgttgagc gtagccagcg
      841 caaaggcgtg gctgatggtc agcttacctt tcagttgcgg cgttttctct accgtttcaa
      901 ccatataatt gatggctgcc acgcccgacg gagtggtttc gtgcaggtga atatcgacac
      961 ctttgtcgta gtccagcgca atctgaaaca tggtgtcgag ggatttttcc atcgcgccat
     1021 cgacactggt cgggtccagc ccgccgacgt aatgcgcacc cgcctgcatc gcttcgcgca
     1081 ttaagggttc cgatttcgac agcagcaaac cgtgctgcgg gaaggcgacg atttcacact
     1141 cgaagcccgc ctgacgccgc gccagcaccg cctgcaaatt ttgcagattt tttaggccgg
     1201 aaaccggttc gatattgcag tggctgcggg caatggtggt gcctttcgac tgcaatagat
     1261 cgatcagttt ttccgcccgc tcctgcgtat acggttgtaa ctccggcagc attttctgct
     1321 caagcttgat catgtcctgg atagtggtgc ctgccggacg attgagcgag cgccacggcc
     1381 cgtcgtagaa ggttttgtcg agatgaatat gcatgtcgcg agtggtgggt agcatcaact
     1441 taccgccagc gtcatagtgc ggcagcgtgg cgtccggatg ctgcttgttc tcgcgcaggg
     1501 cgacaatttt gccgtcctga atctccacgg tctggcgcgc cgtgcgggtc tgcaccgcca
     1561 caccgttttc gtagtcaaaa ccggtttcca gcagcacgtt atcgagatag taatgcgggt
     1621 cagtgatttt catggtgttg ttcgcttcgc agtttagggt gccagctacc gccgcatggg
     1681 cgagcggcac agaggtacca aacagcgcgg cggcggtgac catcttaccg ctctggctca
     1741 gaaattcacg gcggctattg ctttctttca tcctacgtcc ttacgtttta ataatatggg
     1801 tgcccgtttc accgcgcagc gccgcaggca ggcattccgg cgtggtgata atcacttctt
     1861 tgccgccctg ctccaggaac gtcaggctgg cgatgatttt tggcaacatg ctgccgggcg
     1921 gaaaatgccc ttcctgcata tagcgagtca tggtggcaat atccacccga tccagcgcct
     1981 gctgctgcgg tttaccaaag tggatacaga ctttttcgac gccggtggtg atcaccagaa
     2041 tgtcggcgtg aatttcacgg gccagcagcg cggtagagag agctttgtca ataaccgcgt
     2101 ccacgctttg gtaatccccc gcttcagtac gcactaccgg aattccaccg ccgcccgcgc
     2161 caattacgac aaaaccttgt tggatcagcg ccttaatggc gggtgcttcg acaatacgtt
     2221 tcggttccgg cgaggcgacc acgcggcgat agccccgtcc ggcatcttca acaaaacacc
     2281 agtcagggtt tgccttttgt agtttgtcac gctggctttc actgaagaat gcaccaatgg
     2341 gcttggtggg atgggcaaaa cctggatcgt ttttatccac ttccacctga gttaccacgg
     2401 tgacggcttt tttctcgccg tgacgcgcca gccggttatt cagcgcctgt tggatcagat
     2461 agccgatgcc gccctgcgta tctgccacac agttcgccag cggcgttaag ggcagccctt
     2521 cgcgctcgtg ggcaatctcc gcacggcgta aatccagccc gacctgcggc ccgttaccgt
     2581 gggtcagcac aatgtcgtaa tcggaagcca gcatttccag caccgtatcg gccacggctt
     2641 tcaccgcctc cgcctgatgc tcaatcgact ggctggcgtt atctttgata atgctgttgc
     2701 caccaatggc aacgaccaca agctctttca tgttgttatg tccgctacag agggtgatta
     2761 ggttatgacc aacgccagac tgcctgatgc gctacgctta tcaggcctac aattctcggg
     2821 ccatccgtag gccggataag gcgttcacgc cgcatccggc atgaacatgt aggtcagacg
     2881 ccgtaacgtt cgcaccagcc aagaatggct ttctcgaagc aggcaaaagg tggatgcaca
     2941 atgcccgcgc caatcatgcc gacgcccgca tctttatggg cgatggcagt gttgatcacc
     3001 ggcagaatgc cgctgctacc cacgcgggtg atgtcgattg ccgacggcac gcccataaaa
     3061 ccgagcagcg gaatggtgac gttggggttt tcaccgagag tgatttcacg catctggcga
     3121 gagaaatcga tagcctcttc caccgtgccg cccaccagcg caacgattgc aggcgcggtc
     3181 gccatagcaa atccgccaat accgtaggtt tcagtgatgg cgctgtcgcc gatatccagc
     3241 cccgaatctt ccggcttata accggcaaac atcgggccga tgacctgctg cgccggaccg
     3301 gtaaaccatt gccccggtaa tccgctgacc cgcaggccga actcgacgcc gttacgcgcc
     3361 atggtggtga ccacggtgct gtattcgatg ccgtgcgccg catccatcgc cgctttacac
     3421 atcgccatcc acgtcgggcc ggagaagtag tcgctgctag cgacaaactc aaacacttcg
     3481 cgctgtttct ccacggaata acctgcctga ataattcccg gcgtcagcgc ctgaatcagc
     3541 aacgtcgtcc cggcgttatt gcggttatgg cactcatcgc ccatatgcag tgcctgcgcc
     3601 agcattaagc gcagatcaat ctcgccgata attttcatcg cgtcgcgcag cattggcccc
     3661 tgcacatcgc gcatccagtt gaggcggtcg atcacgctct gatcgttggc tcccatacgc
     3721 aaaatcttcg ccatctgctc gctcatattg gtgtaagcga tgttgccgta ggttttgttt
     3781 tttacgatgt gcataaacat cgaggcagag gtaacacccg ccatcgatcc cacacagtcg
     3841 tgttcgtggc acggcgagaa ggtgatctcc ccggaggccg ccagttcagc cgcctcgtcg
     3901 agatctttcg ccagcccttc gaacaccaac gccccggtga ccgcgccttt catcgcgccg
     3961 cacatttttt cccaggtgat cggcggcccg gcgtgaagaa tggttttcgg cgtcatgccc
     4021 ggcaccacgt taatcgcctg atcaaaacca atcagcaccg gatgcgactg gataatgcgc
     4081 tccagcgcct gctggttagc ggcggcgatt ttgtctgcca gcggcgaatc ggcaatgtta
     4141 tccagcgcct gcaccacctg catattgccc tgccccggcg gcgtccagtc gagttgagtc
     4201 acttctacat gctgcttttt caggtcatcg ctaaacatgg cgatgccgac gttaataacg
     4261 ttcaatggtt ggctaaacag tgactggctc atcaggcttc ctcccctttg cagataaatt
     4321 cacgcgccag caatccggta ttggtgctgc tgctcgccag aatcactccg gcatcaagca
     4381 gcatctggct ttgttgttct aacgatggcg tatcaagatc ggtacccagc acataggcga
     4441 gaatgatcaa ctcgcgtcct tcggcagcgg cgatcgcttt cgcttctttg atcgcctcga
     4501 tggtggagcc gaccggatct tcatgcgatc caaatccgag cacaaaatcc atcacgatca
     4561 ccgccacttc tggatcgcgc gcctcttcga tcaagcgact gatgcggttg gtggggtcaa
     4621 tcattggatg cggcttgccg ttcgtgaagt cgtcatcgcc aaagtcgagg aaggtgtgtt
     4681 tgatgctgcg gttgatatct tgcaggcgga attccggatc gggctgaatg ttgctgtaga
     4741 catcgccatg tttttccatc accgcgaaca tggtttcgtc gcacagcgtg ccgccgcaga
     4801 acaggccacg aatgtatttc tgctgcggtt gcagacgcgc acgcacatca gcaatcaacg
     4861 gctggttaag cgtatgcagg tcgagatttt cctgtttcac gccggagagc atcaccgctt
     4921 ttagcgccgc ctctttgctg ccgcgggcaa actgtagccc ctgctcatcc accggcgttt
     4981 caacgcgacc gaggaagcag accaccaccg gcttgcggca ggcacgtgcg cgttccagca
     5041 ctttgcgggc caccgcaggc gcgggaggtt ttgaaatcag tgcgataatt tcagtttgcg
     5101 gatcgttttc cagcatcccg atggcgtcga gcatcatcag gccgccgatt ttctcgctca
     5161 ggtcgcgccc gccggtgcca atcagttgtg aaacaccgcc accaaattca tgaatgcgga
     5221 cgctcagctc ctggctgccg gtgccggatg cgccaacaat accgatattg ccgcgacgca
     5281 cggcgttacc aaaacagagc gccgcgccgt taataatcgc cgtgccgcag tccggcccca
     5341 tcatcagcag ccctttttcg tgggccaact gcttgagcgc cagttcatct tcaactgaga
     5401 cgttatcgga aaacagcatc acgttgagat cgttttgcag cgcctgacgc gcttcgcgag
     5461 cggcaaacag accgttgacc gaaatcaccg ccaggttact ttccgggata tgctttttgg
     5521 cgctggcaat agtggcgtaa cgcgcctcgt gcgagccgct ttgcgctttg gtgttgaaca
     5581 gttcttcaat ctccaccagc aactgctcgt tgtccgcacc cgatttacca ttgatgacaa
     5641 tcatcaggtc gccgtttttc gcctgttcca gctccggcgt cagcagtcct aaatttttca
     5701 gtacaccttt gttcatttcg gtcgccatcg ccacaaatgc ctgctcgacg ccgtcgagtt
     5761 tatttgcacg cgtggagata gacatcagcg agacagaatc aaaataggta ttcggtttaa
     5821 tgactatttt aactgacata attccctccg taggtttggc tcagcgcaca accatcggcc
     5881 atgccataca gcatgtcgca gccggaactg gagccaatat gtttaatttt ttctattgcc
     5941 tgagttgcgt tcccagggat gtcataaata atgttgtgaa taaaacgatg aatattttcc
     6001 cggcaacgtt gttgtaatgc ggcttccagc gttatagcac ttaataatgt ggtgttattt
     6061 ctgccgcgct gcataccgag ataaaattcc tctttgtatt tttccgacgg atgcccggga
     6121 ataaataaaa taagcgccag acctgttaaa taatcgtcgg ctgatggcgt aaggccaatc
     6181 cctaacccaa tcatactggc gaccgctgcg gcgatatttt gtttttcgtt tactgcctga
     6241 ataactgccc gtcgtttaat atgtagttct ttatttaacg cctgataaaa cggattatcg
     6301 ccgcgataat taaacagggt ttcattttcc cccaggtgct gatgaataat gtcgtgccac
     6361 tgtagccagg cgataaggtg aaaattcaca gcggtcattt gcaaggttgg ggacagccaa
     6421 cgccgacaac gactcatctc tatatgaaga tgttgaccaa ccgtaatacc ttgatcgtca
     6481 aactgaacct tatcaccatg ccggaacaga tcatcaaagt gagtgagtgc caaccgacaa
     6541 ctgtttggcg cattgtcgta ctcttcacaa agcagcgtca gcaactgctg gcgagccggg
     6601 agtgcgatat ttaccgctct ggcaaagacc tgttcaacct gtccttgccc ccgctgcgcc
     6661 agaaaatccg catccgcagt tagcgcccac acacaccgtc tttctggtgt gttgatagca
     6721 agcctcccgg tttatgcacc tgcggcaatc agtaactgcg caatctcttc aaagccccgt
     6781 tcccgcgcca gttccagtgg cgttttgcca tatttatcgg tcagatgcgg gctggcaccg
     6841 tgttccagca ataactgcac aatcgcctgc tgtttaatac caccatcatt aagcacaatc
     6901 gcttccagta gcggcgtcca gcctacatgg ttggtctggt taacgttaat ctccgtatgc
     6961 gccaaaagct cttttacaat acttaaatgg cctttttcac aggcaggcgt caggccgaca
     7021 ccgccaaaac gggttacgca attaagatcg ggtttagccg gtaaaataat tcgtagtagc
     7081 gttaaatcat cgttcagaca gctaattaaa aaaggattta aacaggtatg atcttgctta
     7141 ttaatatccg ctccggcatc aattaatgcc tgaacgcaag catattgctg atataaactt
     7201 gccagcgtaa ttgccgtttt cccctgacga tcgcaggtat taatatcgac acccagcgca
     7261 aggcaggttt ttactttatc gatatcaccc tgttgtgcag ccaataaata gtcggcaggt
     7321 agatttttta tactcattca cattcccctg gtttttgtgg ctgttttcga tgaattgaga
     7381 atagcaacga taatctgata aaagaacccg cttaaattta attcgctgta tttcgccttt
     7441 ttgttgaatg atgttcaaca atcttttcgc agtgtgcggt agtgcaatat ttgtttcttt
     7501 gacccgatac cctttcactc aatcaggttt tatttctcta tagtctgcac atgtaagagt
     7561 agctttcgtc catgtctaac cagaggggaa caagatgaac ggactcacgg caacgggtgt
     7621 caccgtcggt atttgtgcag ggctttggca gttagtgtct tcacatgtcg ggctctctca
     7681 gggatgggaa ttattaggaa ccattggctt cgtcgccttt tgcagttttt atgccgcagg
     7741 cggcggtaaa tcggggttta tcagaagtct ggcggttaat tattccggga tggtgtgggc
     7801 atttttcgcc gcgctggctg ccggttggct ggcctctgta agtggactgt ccgcgttctg
     7861 ggcgagcgta attactaccg tacccttctc ggcggtagtg gtctggcaag ggcgtttctg
     7921 gttactgtcc tttattcccg gtggctttct cggcatgacg ctgttttttg ccagcgggat
     7981 gaactggacg gtgacgttac tgggttttct ggcgggtaac tgcgtgggga taatttcaga
     8041 atatggcgga caaaaattaa gcgaagcgac gactaaacgc gacgggtatt aaaagttatt
     8101 caaaaatgat tatctatctt taatcgatac ctctacagtc atggctggag aaattcatat
     8161 tagataatgg cgggaatggt ttaatgaact cagtttttac cgaagagaat ctgttggcat
     8221 ttactaccgc tgcgcgtttt ggcagtttca gtaaagccgc agaagagttg ggtttaacca
     8281 cttccgccat tagctacacc atcaagcgaa tggaaacagg gctggatgtg gtgctgttca
     8341 ctcgcagtac ccgcagcatt gagttaacgg agtccgggcg ttattttttc cgcaaagcta
     8401 ccgacctgct gaatgatttt tatgccatca aacgtagcat agatactatt tcgcagggca
     8461 tagaagcgcg tgtgcgcatt tgtattaatc agctgcttta tacgcccaaa cacaccgcgc
     8521 gcttattaca ggtgctgaaa aagcagtttc ctacctgcca gattaccgtc accaccgaag
     8581 tgtataacgg cgtctgggat gcgattatta ataatcaggc caatatcgcc atcggcgcac
     8641 ccgatacact actggacggc ggcggtattg attacactga aattggcgcg attcgctggg
     8701 cttttgctat cgccccggat cacccgttag cctttgtgcc ggaacccatc gccgaaagcc
     8761 aactgcgtct ctaccctaat attatggtgg aggacaccgc gcatacgatt aataaaaaag
     8821 tgggctggtt gctgcacggg caggagtcaa ttctggtgcc agatttcaac accaaatgtc
     8881 agtgccagat tttaggtgaa ggaattggtt ttttgccgga ttacatggtc cgtgaggcga
     8941 tgacgcaatc cctgctggtt acccgacaaa tccataatcc gcgccaggat tcgcgcatgt
     9001 tactggcgac tcagcattct gctacgggtc aggtgacgca gtggattaaa aaacaatttg
     9061 cgccaaatgg catattaacg gggatctatc aggatttact gcatcgggag aattaatcgc
     9121 tgccaacctc ccgatgcgct gtgaatagcg gtttacatca accacctgct ttcattaccc
     9181 attcagagat aaatttatta cccggtactg gcggagagta caaatagccc tgcaaaaagt
     9241 gaacgccttt accgatcatt aaatccgcct gctcctgggt ttctacccct tccgccacga
     9301 tactcagacc aagcttacgc gctagttcga caatattgtc cacaatatga ccggagattt
     9361 cgtcgacact cgccatttgc acaaatgact tatcgatctt aataaaatcg accgggaacg
     9421 cctgcaagta acgataggtc gcataacccg taccaaagtc atccagcgca aaggtaatgt
     9481 tgtgctgatg aaggctgtca aatatcgctc tggcttctgg cgttaccgga atagggttac
     9541 gttccgttag ctcgagaacc agcttgattt tatcgttacc taatttctta accaggttca
     9601 gacactcttt ttcaaatccc gctgccaaaa aacaacccgc cgagacgttg atgccaatat
     9661 ggaaattgtc cggcagcaaa tgttttaccg gcatcagaat atccgcagtc tgtttcatca
     9721 gttggcgggt cattatgaca ataagaccgg atgactccgc cagaggaata aactgatccg
     9781 gtgggataat tcccgtttgt ggatgttccc agcggacaag tacctcacag cccgtcagta
     9841 ccccagtctg cgcgcagaaa accggttgga tccacggttt gaattcgtgg ttttccaacg
     9901 ccagactgat ggcttcaggc gtgacgatat ggtgacaacg gttatcaggg atataaaaat
     9961 tatttttatt tcccgtgcca ctgatgactt gcggatgata ttgaaaaaag atatcccgcc
    10021 agaaggtaat atcactctgg atgcctagtt tttcatacag ctgcttcttt tgatgtgaaa
    10081 ttgtctttgc actgcgattt ctgtactgtg agatttctgt aaccgacatt ccctccatta
    10141 gcagcttgag taaaagccgt tctttttcgg acacgatacc aggcaacgtc aaattcaccg
    10201 ttgccccgaa ctcttccaaa aaaacactca aatcatgcga agccatcgaa cacaccttga
    10261 ttaaatcttg tgcgccagct aaatctgcga gtcttcactc tgactgataa agctggctgc
    10321 aagaaaatga cacagagagc cgaattatct cccgttcggc tctctctcct tttactcatt
    10381 attcagatca gaattgccac ttaatgccaa ccattgcaga ggtgtcatta tatcccctat
    10441 cggcaacctg aacgcccaca ttcccccaga cattcaggtt ggcatttagc tgtccttcca
    10501 ccccggtttt tatctcagca atatttcggg ctccatcctg agtgacggac acgccatcca
    10561 tactggtgct gaaatcctta ctgttatgca gccagttcac ttctacaaac ggctggaact
    10621 cgcgggattt accgtcatcc attttatggt ggctcttcag ccaggttttt acgccaagtc
    10681 gggtttgaac attgccatca ccgttgctat gaacgagggt tccgttgctt tcgcggtgct
    10741 tatcggcttt gactcccatc caggtaacct gtgcttgcgg ctgaacatac cattcattac
    10801 gcgttccctg gctgccatta aattcagcta atttgtgttt gtatccagct tccagtgaag
    10861 cggtaaatcc ttttgattta taggattcac tttgtaagtc atcccctttc actgtgttat
    10921 caaaccagct gtactgcgcc caactgtcga gatacgcgcc attacgcgac tcgtcatcgg
    10981 cataccaggt ggcatagagg ccggtgctat atccgttcac actcgctttt gcacgataac
    11041 cggttcgcga ggaaatggtt ttactgtcgc tgttgccata tcccgccatg accccaacat
    11101 gccagctgtc gctgccgttt tggctccact gcgcgacatc gcctcccagt tgcagaacat
    11161 agcgattgct ttgggttttc agctggccgc tgccatcacg ccatttatta tgaccacctt
    11221 catggcgcat ccacatagtg gtttgtttct gctcacccgt caccatgtcg gtatagtacg
    11281 tattacccag acgctcatgc agacgcgtgg tgaacattgt attcgctgct gcaaggttcg
    11341 caatgtagct acccgcctcc ggtcgcaggt cattatccac attcagatcc ggacccggcg
    11401 taggtgtcgg gttagggttc ggctctggat tcgggtttgg ctccggattc ggcatcgggt
    11461 ctggctccgg ctgcagttca ggagaatcac tgccgctggt cagataccag ttaccactat
    11521 ttgccccttg tccacgcgcg agagtgtagt catacgcccc cgcaacgata cgaccagcct
    11581 gaacaaattc gccctcagac ttaccgtcta catggataag ttcgataccg ttaagggttt
    11641 tcgcacctgt accgccagcg ttattcaccg taacggcagt cgtgccgcta gtatcgccct
    11701 cgacaaccaa tttatcggtt actgagtcat cgccattcag taccgtattc attactagtt
    11761 gaccgttatt accgtggtag ttccccttca ccactaacac attgccagct tgctgtcctg
    11821 acattccggt catcagcgta ccgttgttgg tcagattgcc gcccagggtg taattcacag
    11881 acgcagccgc tcttttttca gcgctattat tcaacgccag ggtaccgttg ttctcaacgt
    11941 ttgcgctacc caatccctgc gcatgactta ccaggacatt agccgaatta gcaatgttca
    12001 cattcgctgt caggttgctg ttatcgcccg cgatgtcaag cgtgccgccc tgaatattca
    12061 gctctccgct ccccgttaac gaaccggttg aagtgccccc gttggtaacc gtcagactgc
    12121 cgccatttaa gctcagcagt gaatcggcgc tgccattgag cgtgcccacg gtctggctgt
    12181 gaccattcat atccagttca gtctctgccg ccagacgcag ttcatgggta ttacccaaca
    12241 cgttatcgtt attcaacaaa agcgtcccac tgcgcagatc ggtaacaccg gtatagtcgt
    12301 tgtctttgtt agaaagcgat acggtctgac ccttctggct gctgaatgcc aggtcaccac
    12361 tgccggtaat ctttgcgctg agatcggcgg cattctcgct tttaccgtta gagctcagca
    12421 ccagagcatc gctgtcggta gcgtgtaaat caagctgggt caggccgtaa ccgatataca
    12481 gaccgtcatt gtttacccca ctcgtcagac gatagtcgta agtgccgacg gccgcttttt
    12541 gcccattctg ctgaatatca cgttgggcac tgctggagag gatttgccca tttttatcct
    12601 gcaattgcag attgcccgca tcgcccagaa cttccgcacc ttgcgcatca accaacttaa
    12661 tggtgctgtt accatcatcg acttcagtga gcgataacgc agagtcaata tcacgagata
    12721 ctgagcttac aacgtcactg tcagaaacct gaatcacacc ctcgccgcgc aaatccagcg
    12781 ttttactaac gttgaccgtc ccttctgtca tctgtgcacc tgctgtcagg gcaccaaaat
    12841 caacggttcc gccggagaat cccaaagtgc taatggagtg agcgccctta cccactgtta
    12901 ccaggcttcc ctgacccgca gtgatcccgc ctctctgcag aaactcagca ttcttttcca
    12961 ggttaaatgt cgtgttctgg aacgccagtg aaccctggaa ccagtctgta taagcaaact
    13021 catcgaagga gaattcgtga ttccccatgt ttatttcaac aatacctcta ccttgaagat
    13081 cgttatcaaa tacccaatct ttattggcat tgatttgcag tttgccatca acatccaggt
    13141 ccgcacggcc gatgttcttg tcttcagaaa taaccgcgac agcaccagaa gcgacatcaa
    13201 tatatccgct gaatgcatag ttattgccgg tcagtgccac tgtcccactg tcgatactta
    13261 ttgtaccatt gccggaaatc ttattggcaa aggtcatgct atcgtcagcc ttcaggtgta
    13321 atttcccgcg gttgttgatc tctgctgtac ctatattttt ggtgatccct aaagcgccat
    13381 tttgctcaat tttgacttta ccactaaagg cagagttatc accggttaag taagtcgtgc
    13441 cattgagcgc attgatatag cccaggccct cgctgccact gacaatatcc gcggaaatag
    13501 tcgcgttttt atccgtgtgg tttaaggtga ttttcttatc ggatgtcttt gcccacacaa
    13561 actcaatttt ttcggcatca ataatccctg cagccttcgc agcacttcct tcctgtgcac
    13621 ctaacgctaa ctcagagttg gtgcttaaac taattttcgg tgcggatatt ttacctccat
    13681 ctgatacgac taatgttgta tcagaacgtt ggtcgtaata acccaggcta atatgttcac
    13741 gagcattaac ttcaccaccg ttattaaccg acaggaactg attaccgcca tatccgccgt
    13801 aaacgcccat aaagaaggtc cctgcattta cctgactacc ttcgccgctt acattcccct
    13861 gcgtataagt cagacgactg gtgcccttaa catgaatttt gccgccattg gagacattaa
    13921 aatagccttg atcatgtctc atgcctgagt ttgactcata caaatcatca gcatataact
    13981 cgctgccagc attggagaca ttcaggaacc ccgtaccttt cagcaaaaag ttaagcgggc
    14041 cattgattgt tacctttcct ccggtaatat ccaacccacc accagaagcg agagtcagac
    14101 gcggacggga agtatctgtc gtattgatta ccagattggc attgtcagtc accgtaatac
    14161 ggtaatcgat gctctggttc tctttgccaa aatcaatagc tttatcctgg tttacgatga
    14221 catcatcggc gaataccaga gatgaatata aacttatcgc gctaattaaa acagcccggg
    14281 acttttttcc ttttactctg ctccccagct ccgagcagac gctccaacag ttacgggacc
    14341 tgttccactt tagccgatat attttattca tcacaatctc cagcaataca tccaaataaa
    14401 accaagattg cataaacagc aacgcattaa atgccattct ggttactgtt catcgcatat
    14461 aaaaattcaa aaattttgca tagcattggc aaccacatat ctgagtatgt agttcagagg
    14521 gtaataaaat agcaagttac tactgaaaac ttattttaag ttaacactct taactttatc
    14581 attacattcc tcccagtctc taatacc
//
LOCUS       DAZFVO020000127        14280 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFVO020000127 DAZFVO020000000
VERSION     DAZFVO020000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.128"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            255..1313
                     /gene="citC"
                     /locus_tag="ACHKNB_RS18205"
                     /old_locus_tag="pgaptmp_003641"
     CDS             255..1313
                     /gene="citC"
                     /locus_tag="ACHKNB_RS18205"
                     /old_locus_tag="pgaptmp_003641"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HGU4197084.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1328..1624
                     /gene="citD"
                     /locus_tag="ACHKNB_RS18210"
                     /old_locus_tag="pgaptmp_003642"
     CDS             1328..1624
                     /gene="citD"
                     /locus_tag="ACHKNB_RS18210"
                     /old_locus_tag="pgaptmp_003642"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HGU4197085.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1621..2529
                     /gene="citE"
                     /locus_tag="ACHKNB_RS18215"
                     /old_locus_tag="pgaptmp_003643"
     CDS             1621..2529
                     /gene="citE"
                     /locus_tag="ACHKNB_RS18215"
                     /old_locus_tag="pgaptmp_003643"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HGU4197086.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2540..4072
                     /gene="citF"
                     /locus_tag="ACHKNB_RS18220"
                     /old_locus_tag="pgaptmp_003644"
     CDS             2540..4072
                     /gene="citF"
                     /locus_tag="ACHKNB_RS18220"
                     /old_locus_tag="pgaptmp_003644"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HGU4197087.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4076..4627
                     /gene="citX"
                     /locus_tag="ACHKNB_RS18225"
                     /old_locus_tag="pgaptmp_003645"
     CDS             4076..4627
                     /gene="citX"
                     /locus_tag="ACHKNB_RS18225"
                     /old_locus_tag="pgaptmp_003645"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HGU4197088.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4602..5480
                     /gene="citG"
                     /locus_tag="ACHKNB_RS18230"
                     /old_locus_tag="pgaptmp_003646"
     CDS             4602..5480
                     /gene="citG"
                     /locus_tag="ACHKNB_RS18230"
                     /old_locus_tag="pgaptmp_003646"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HGU4197089.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5531..6994
                     /gene="citT"
                     /locus_tag="ACHKNB_RS18235"
                     /old_locus_tag="pgaptmp_003647"
     CDS             5531..6994
                     /gene="citT"
                     /locus_tag="ACHKNB_RS18235"
                     /old_locus_tag="pgaptmp_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HGU4197090.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7108..7914
                     /gene="rna"
                     /locus_tag="ACHKNB_RS18240"
                     /old_locus_tag="pgaptmp_003648"
     CDS             7108..7914
                     /gene="rna"
                     /locus_tag="ACHKNB_RS18240"
                     /old_locus_tag="pgaptmp_003648"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HGU4197091.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8144..8554
                     /gene="rnk"
                     /locus_tag="ACHKNB_RS18245"
                     /old_locus_tag="pgaptmp_003649"
     CDS             8144..8554
                     /gene="rnk"
                     /locus_tag="ACHKNB_RS18245"
                     /old_locus_tag="pgaptmp_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HGU4197092.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8692..8781
                     /gene="yldA"
                     /locus_tag="ACHKNB_RS18250"
                     /old_locus_tag="pgaptmp_003650"
     CDS             8692..8781
                     /gene="yldA"
                     /locus_tag="ACHKNB_RS18250"
                     /old_locus_tag="pgaptmp_003650"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HGU4197093.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYFENHS"
     gene            complement(8785..10023)
                     /gene="ybdR"
                     /locus_tag="ACHKNB_RS18255"
                     /old_locus_tag="pgaptmp_003651"
     CDS             complement(8785..10023)
                     /gene="ybdR"
                     /locus_tag="ACHKNB_RS18255"
                     /old_locus_tag="pgaptmp_003651"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HGU4197094.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10244..10672
                     /gene="uspG"
                     /locus_tag="ACHKNB_RS18260"
                     /old_locus_tag="pgaptmp_003652"
     CDS             10244..10672
                     /gene="uspG"
                     /locus_tag="ACHKNB_RS18260"
                     /old_locus_tag="pgaptmp_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HGU4197095.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10793..12358)
                     /gene="ahpF"
                     /locus_tag="ACHKNB_RS18265"
                     /old_locus_tag="pgaptmp_003653"
     CDS             complement(10793..12358)
                     /gene="ahpF"
                     /locus_tag="ACHKNB_RS18265"
                     /old_locus_tag="pgaptmp_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HGU4197096.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12487..13050)
                     /gene="ahpC"
                     /locus_tag="ACHKNB_RS18270"
                     /old_locus_tag="pgaptmp_003654"
     CDS             complement(12487..13050)
                     /gene="ahpC"
                     /locus_tag="ACHKNB_RS18270"
                     /old_locus_tag="pgaptmp_003654"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HGU4197097.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13422..14168
                     /gene="dsbG"
                     /locus_tag="ACHKNB_RS18275"
                     /old_locus_tag="pgaptmp_003655"
     CDS             13422..14168
                     /gene="dsbG"
                     /locus_tag="ACHKNB_RS18275"
                     /old_locus_tag="pgaptmp_003655"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HGU4197098.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3407 a         3770 c         3576 g         3527 t
ORIGIN      
        1 ctgctggtca tttcgtaatt acattaatta aaaaacttaa aaccattaaa taaataaaaa
       61 accactaact ctatgtgaaa taaatcaaaa tttcacgccg aaatactcct taggatgtat
      121 agcgaaagga gaaaaagata tacctcgatc accccccttt ctcccaagtg aaaataaaag
      181 gttatcagtt tgcaacattg aacaacattc gttgcaaatc gataacaaca tgcaccttca
      241 ggatactatt tattatgttc ggcaatgata ttttcacccg cgtaaaacgt tcagaaaata
      301 aaaaaatggc ggaaatcgcc caattcctgc atgaaaatga tttgagcgtt gacaccacag
      361 tcgaagtatt tattaccgta acccgcgatg aaaagcttat cgcgtgcggt ggaattgccg
      421 gaaatattat taaatgcgtt gctatcagtg aatccgttcg cggtgaagga ctggcgctga
      481 cattagccac tgaactgata aacctcgcct atgagcggca cagcacgcat ctgtttattt
      541 ataccaaaac cgaatacgag gcgctgttcc gccagtgcgg tttttccacg ctgaccagcg
      601 tacccggcgt gatggtgctg atggaaaaca gcgccacgcg gctgaaacgc tatgccgaat
      661 cgctgaaaaa atttcgtcat ccagggaaca agattggctg cattgtgatg aacgccaatc
      721 ccttcacgaa tggtcaccgt tatctgattc aacaagctgc ggcacagtgc gactggttgc
      781 atctgttttt agtcaaagaa gattcttcac gcttccccta tgaagaccgg ttggatctgg
      841 tgttaaaagg caccgccgat attccacgcc tgactgtgca tcgcggctcc gaatacatca
      901 tctcccgcgc tacgttccct tgctacttca ttaaagaaca gagcgtcatt aaccattgtt
      961 acaccgaaat tgatctgaag attttccgtc agtacctcgc tcccgcgctg ggtgtaactc
     1021 accgctttgt cggtactgaa cccttttgtc gcgttaccgc ccagtacaac caggatatgc
     1081 gctactggct ggaaacgccg actatctccg caccgcccat cgaactggtt gaaattgagc
     1141 ggctgcgtta ccaggagatg ccgatatccg cttcccgggt acgtcaactg ctggcgaaaa
     1201 acgatctcac ggctatcgcg ccgctggtcc ctgcagtcac gctgcattat ttgcagaacc
     1261 tgcttgagca ctctcgccag gacgcggcag ctcgtcaaaa gacccccgca tgagaaacag
     1321 gtgaaaaatg aaaataaacc agcccgccgt tgcaggcacc cttgagtctg gggatgtgat
     1381 gatacgcatc gccccactcg atacgcagga tatcgacctg caaatcaata gcagcgttga
     1441 gaaacagttt ggcgatgcaa ttcgcaccac cattctggac gttctcgccc gctacaacgt
     1501 gcgcggcgta cagctgaatg tcgatgacaa aggcgcactg gactgcattt tacgtgcacg
     1561 actggaagcc ctgctggcac gcgccagcgg tatcccggct ctgccatggg aggattgcca
     1621 atgatttccg cttcgctgca acaacgtaaa actcgcaccc gccgcagcat gttgtttgtg
     1681 cctggtgcca atgccgcgat ggtcagcaac tccttcatct acccggctga tgccctgatg
     1741 tttgacctcg aagactccgt ggcattacgt gaaaaagaca ccgcccgccg catggtttat
     1801 cacgcgctgc aacatccgct gtatcgcgat attgaaacca ttgtgcgtgt caacgcgctg
     1861 gattccgaat ggggtgttaa cgacctggaa gccgtcgttc gcggtggtgc ggacgttgtg
     1921 cgtctgccga aaaccgatac cgctcaggat gttctggata ttgaaaaaga gatcctgcgt
     1981 atcgaaaaag cctgtggtcg tgaacccggc agcaccggcc tgctggcggc gattgaatct
     2041 ccgctgggca ttacccgcgc agtggaaatc gctcacgctt ccgagcgttt gatcggtatc
     2101 gccctcggtg cagaagacta tgtgcgcaac ctgcgtacag aacgctcccc ggaaggaact
     2161 gaactgctgt tcgcacgctg ttccattttg caggccgcgc gctctgcggg tattcaggcg
     2221 ttcgataccg tctattccga cgctaacaac gaagccggat tcctgcaaga agccgcccac
     2281 atcaaacagc tgggctttga cggcaaatcg ctgatcaacc cgcgtcagat tgatctgttg
     2341 cacaacctct acgcaccaac ccagaaagaa gtggatcacg cccgccgcgt cgtagaagcc
     2401 gctgaagccg ccgctcgcga aggcctcggc gtggtttccc tgaacggcaa gatggtggac
     2461 ggtccggtta tcgatcgcgc ccgtctggtg ctctctcgtg cagaactttc cggcatccgc
     2521 gaagaataag gcaatcaaaa tgacgcagaa aattgaacaa tctcaacgac aagaacgggt
     2581 agcggcctgg aatcgtcgcg ctgaatgcga tcttgccgct ttccagaact cgccaaagca
     2641 aacctaccag gctgaaaaag cgcgcgatcg caaactgtgc gccaacctgg aagaagcgat
     2701 tcgtcgctct ggtttacagg acggcatgac ggtttccttc catcacgctt tccgtggcgg
     2761 tgacctgacc gtcaatatgg tgatggacgt catcgcgaag atgggcttta aaaacctgac
     2821 cctggcgtcc agctccctga gtgattgcca tgcgccgttg gtagaacaca ttcgccaggg
     2881 cgtggttacc cgcatttata cctccggcct gcgcggtcca ctggcggaag aaatctcccg
     2941 cggtctgctg gcggaaccgg tgcagatcca ctctcacggc ggtcgtgtgc atctggtaca
     3001 gagcggcgaa ctgaatatcg acgtggcttt cctcggcgtc ccgtcctgtg atgaattcgg
     3061 taatgccaac ggctacaccg gtaaagcctg ctgcggctcc ctcggctatg caatggttga
     3121 tgccgacaac gcaaaacagg ttgtgatgct taccgaagaa ctgctgcctt atccgcataa
     3181 tccggcaagc attgagcaag atcaggttga tttaatcgtc aaagttgatc gcgttggcga
     3241 tgctgcaaaa atcggcgctg gtgctacccg tatgaccact aacccgcgcg aactgcttat
     3301 tgcccgtagc gctgcggatg tgattgtcaa ctcaggctac ttcaaagaag gtttctccat
     3361 gcaaaccggc accggcggcg catcgctggc ggtaacccgt ttcctggaag acaaaatgcg
     3421 tagccgcgat attcgcgccg acttcgccct cggtggtatt accgcgacga tggttgatct
     3481 gcacgaaaaa ggtctgatcc gcaaactgct ggatgtacag agctttgaca gccatgctgc
     3541 gcaatcgctg gcccgtaacc ccaatcacat cgaaatcagc gccaaccagt acgctaactg
     3601 gggttcgaaa ggcgcatcgg ttgatcgtct cgacgtggtg gtactgagcg cgctggaaat
     3661 tgacacccag ttcaacgtta acgtgctgac cggctctgac ggcgtactgc gtggtgcttc
     3721 cggtggtcac tgcgataccg cgattgcctc tgcgctttcc atcatcgtcg cgccgctggt
     3781 acgcggtcgt attccgactc tggtggataa cgtgctgacc tgcatcaccc cgggctccag
     3841 tgtcgatatt ctggtcacag accacggtat cgcagttaac ccggcacgtc cggaactggc
     3901 agaacgtctg caggaagcgg gcattaaagt ggtttccatt gagtggctgc gcgaacgtgc
     3961 gcgtctgctg accggtgaac cacagccgat tgaattcacc gaccgcgtcg ttgccgttgt
     4021 gcgttaccgc gatggctcgg tgatcgatgt tgtgcatcag gtgaaggaat aagccatgca
     4081 cctgcttcct gaactcgcca gccaccatgc ggtgtcaatt cccgagctgc tcgtcagccg
     4141 ggatgaaagg caagcacggc aacacgtctg gctcaagcgc catcctgttc cactggtctc
     4201 ctttaccgtg gttgcgcctg ggccgattaa agacagcgag gtcacacgcc gaatttttaa
     4261 tcatggcgtg acagccttgc gtgccttagc cgcaaaacag ggctggcaaa ttcaggagca
     4321 ggctgcactg gtttccgcca gcgggccgga gggcatgttg agcattgccg ccccggctcg
     4381 cgacctcaag ctcgccacca ttgagcttga acatagtcat cctctcgggc ggttatggga
     4441 tatcgatgtc ctgacgcccg aaggcgaaat tctctcccgc cgcgactatt cactgccgcc
     4501 tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa aaacccatca
     4561 actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg cctgcaacgt
     4621 caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg ctgggctggc
     4681 gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat cgcattaact
     4741 gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg gcgattcagg
     4801 gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca ccggaagcgg
     4861 tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc cgcgccactg
     4921 cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt gcggcaattg
     4981 gtcgtttgct tcaactcaac caaccggtaa cgccaacaac cgtttgttct acggcggcaa
     5041 gtttctgccg tggcctgacc gatcgagaac tgcgtaccaa taattcacaa ctgacggcag
     5101 gtcaacggtt gtaccaacag cttggcctta ccggcgcacg cggtgaagcc gaagcgggtt
     5161 atccactggt gatcaatcac gccttgccgc attacctcac tctgctggat caggggttag
     5221 atcctgaact ggcattgctc gataccttgc tcctactgat ggcgatcaac ggcgatacca
     5281 acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag gcgcaaacat
     5341 tattgcaaaa agggggcatt cgaacccccg ccgatctcga ttatctccgg cagttcgaca
     5401 gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg atccttacct
     5461 ggtttttagc acagatttaa ttatttaagc acttgataaa tttggaaata ttaattttcg
     5521 gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc cactggtggt
     5581 gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccaccacagg catggcatta
     5641 cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc cggcaacagc
     5701 gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc tctttgatgc
     5761 caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat ggggtctggc
     5821 tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg cattagggta
     5881 tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca tgggcaaacg
     5941 cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac cgtttacacc
     6001 gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc tgccgccgct
     6061 gtttaaatca ttccccaacg atccgtccgc gcgtcgtatt ggcggctatt tgatgtggat
     6121 gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg caccaaacgt
     6181 gctgggtctg gagttcgtca gcaaaattgc cggtatccag attagctggt tgcagtggtt
     6241 cctctgcttc ctgccggttg gggttatctt gcttatcatt gcgccgtggc tttcctacgt
     6301 gctgtacaaa ccggaaatca cacacagtga agaagtggca acctgggcgg gtgatgaact
     6361 aaaaaccatg ggtgcgctga cacgcagaga gtggacgctg attggccttg tattgctcag
     6421 cttaggtttg tgggtatttg gcagtgaagt cattaatgct actgcggttg gtctgctggc
     6481 agtttcgcta atgctggctc tgcacgttgt gccgtggaaa gacattaccc gctataacag
     6541 cgcatggaac acgctggtca acctggcaac tctggttgtg atggctaacg gcctgactcg
     6601 ttctggtttt attgactggt tcgccggtac catgagtacg cacctggaag gattctcacc
     6661 aaacgcaacg gtgattgtac tggttctggt gttctacttt gcacactacc tgtttgccag
     6721 cctgtctgcg cacaccgcaa ccatgctgcc ggttattctg gccgtcggta aaggtattcc
     6781 gggcgtacca atggaacaac tgtgtatcct gctggtgctg tctatcggta tcatgggctg
     6841 tctgacgccg tatgcaaccg gtcctggggt gattatttac ggctgtggct atgtgaaatc
     6901 aaaagattac tggcgtcttg gcgcaatctt cggggtgatt tacatctcta tgttgctgtt
     6961 ggttggctgg ccgattctcg ccatgtggaa ctaatacgtt cgccaaacgc tgaataaatc
     7021 atcacgcccg ccaggtaagc ccacctggcg ggctttttta tgatttaata gatagtattc
     7081 agtgtatgta tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc
     7141 gcggtttctc tgcttccctt ctcttctgcc aacgccttag cgttgcaggc aaaacagtat
     7201 ggcgattttg atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa
     7261 cacgatcgaa atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa
     7321 gctgattttc tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt
     7381 ggtgttgatg aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta
     7441 ccagaagcgc gcgccagccg aatgtgttca tcgccggaaa ccggattatc actggaaacg
     7501 gccgctaaac taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac
     7561 gaatatgcca aacacggtgc ctgctttggt tttgatccgg acgcatactt cggtacgatg
     7621 gtacgcctga atcaagaaat taaagagagc gaagccggaa aattccttgc ggataattac
     7681 ggtaaaacag tgagccgccg tgactttgac gccgcctttg ccaaaagctg gggaaaagag
     7741 aacgtgaaag cagttaagct aacgtgccag ggtaaccctg cgtatttgac tgaaattcag
     7801 atctcgatca aagctgacgc catcaacgct ccgctttctg caaactcatt cttgccacaa
     7861 ccacacccag gcaactgtgg caaaaccttt gtgattgata aagtgggtta ttaatcatct
     7921 gatttacaaa acacgctaat tcgtcttcca taaaccgtga cgattaagaa ttatacataa
     7981 tgcgctgcat tgaggaattc cctgaatgtg acgcaaatca cttcaaagag tgagcaacag
     8041 tcagtatcat catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt cttttgggta
     8101 tgtttatctg cgacactcgc agtaccgacg acatggagta aaaatgtcca gaccaactat
     8161 catcattaac gacctggatg ccgaacgcat cgatattctg ctggagcaac ccgcctatgc
     8221 tggtttgcca atcgccgacg cgttaaacgc agagttggat cgcgcccaaa tgtgttcgcc
     8281 agaagagatg ccacacgacg tggtgacaat gaacagccgg gttaaattcc gcaatcttag
     8341 cgatggcgaa gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg atagcaatac
     8401 tcagctttcc gttatggctc cggtaggtgc cgcactgctg gggctgcgcg ttggcgattc
     8461 cattcactgg gaacttccgg gcggcgttgc aacccacctt gaagtgctgg aactcgaata
     8521 ccagccagaa gctgctggcg actacctgct ttaatcccat ctgcacaagc gataactcgc
     8581 tttgcccaga ggatgcactc gcatcctctt tacgcaaaat cagcaatttt tcctggttct
     8641 cactgttcac ttttgctgca aaattattcc gtttcgtttg tgggaataac catggcagaa
     8701 gcattttaca tcctgatagg atttctgatt atggcggcga ttatcgtcat ggctgtcctt
     8761 tacttcgaaa accactcctg acgatcatat tgttcccccc ggcatcgcat tcaccagacc
     8821 tgaaaccgcc ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa tcaccttacg
     8881 gcactcctct tcacgttttt cgaaaatctc atatccccgg gcggcctctt caaacggcat
     8941 atagtgggta acaatttctt ctggtttcag taatcctttc tcaattaacg gtaataattc
     9001 tcccagccat gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt tatcaaaggc
     9061 gtcgccaaac aggaaaccgt gaataaatcc agcgtagacg cccggtacgc taacaatgcc
     9121 gccacgcctg accgccgcaa tacactggcg caacgcttta ccgctgctgc cctccagttt
     9181 caggttagtc agcaccgttt ccgtggtgct gcctttcgct tcaaaaccga cggcgtctat
     9241 tactgcatcc acgccccggt gacctgccgt ttgttcaata attgactgtg ccggatcgct
     9301 atcttcatcg aaattaatcg ggatcgcgcc gtagcggtca gcggcgaaac gcaagcggta
     9361 gggatgatga tcaacaacaa aaatctgttc cgcaccgagc aaccgtgcac aggcgattgt
     9421 caacaatccc actggaccgg caccatagac tgcaacgctt gaaccttgtt ggatctgcgc
     9481 attttttgct gcctgccatg ccgttggcag aatatcagaa aggaaaaggg ctttatcatc
     9541 tgaaagcaaa ggcggtactt taaacggccc cacattccct ttagggacgc ggacatattc
     9601 cgcctgccca ccaggaacgc cgccatacag gtgactataa ccaaacaatg ccgcgggagc
     9661 tggtatctgt tttttattga gcgcagcgcc tttacccgca ttggtatttt cgcaggcggc
     9721 atattgctgt aatcgacaga aaaaacagtc gccacaagca atgacaaacg gaattaccac
     9781 ccggtcgcct ttttgcaaat tttttacgtc ctttccggtt tcaacgactt cccccataaa
     9841 ttcatgacca aaaatatcgc catgtttaac ctggggtatt ttgcctcgat aaagatggag
     9901 gtcagagcca cagatcgccg ttgccgtaat acgcagaata atatcatctg cctgttcaat
     9961 gcccggatcg ggaacatttt ctacctgaac gtgatgtggg ccgtgataag tcaatgcttt
    10021 catgcaacct ccagagacag ttcaaaaaag agtcagtctt taagggtagc tatcacagcg
    10081 ggaaaacggg cggtaccgca ggttttggga gaattcctgg tgttcagtga gaatatatta
    10141 atcacagaaa ccgttcgtaa ggaaaaagaa ccagacaaat atcagggata aatattataa
    10201 tcatggcgaa tgatttgttc atgattaaca gggagaaagg gttatgtata agacaatcat
    10261 tatgccagtt gatgtatttg aaatggaatt gagcgacaaa gctgttcgcc acgctgaatt
    10321 cctcgcccag gatgacggag ttattcatct acttcacgta ctacccgggt cagccagcct
    10381 gagcctgcac cgttttgccg ctgatgtgcg tcgttttgaa gagcatctgc aacatgaagc
    10441 agaagaacgt ctgcaaacga tggtcagcca cttcaccatc gatccttccc gcattaaaca
    10501 acatgtccgt tttggtagcg tgcgggatga agtcaatgag ttggcagaag aactgggggc
    10561 tgatgttgta gttattggtt ctcgcaaccc atcgatttcg acccatctgt taggttctaa
    10621 cgcctcgagc gtgatccgcc atgccaatct gccggtgctg gttgtgcgtt aattgccaga
    10681 ataaatatcc cgccctgccc ggagccatcc cggcagggct ttttaatgcc ataaaaaagc
    10741 cgccaggttt gaccccggcg gctaagcaat tgcaggtgaa tcttacttct tcttatgcag
    10801 ttttggtgcg aatcaggtag tcaaaagcac tcagagaggc tttggcacct tcgccagtgg
    10861 cgatgatgat ctgcttgtac ggaaccgtcg tacagtcacc cgctgcgaat acgcctttga
    10921 cgttggtttc gcatttcgca tcaatgataa tctcacccat gcggttacgt tcgactgcgc
    10981 cttccagcca gttggtgttc ggcagcagac caatctggac gaaaataccg gccagttcga
    11041 tgttgtgaat atcgccgctg acacgatctc gatattccag accaacgact ttgctgccgt
    11101 cgcctttcac ttccgtggtt tgcgcattca gaataatgtc gacgtttttc aggctgcgca
    11161 gtttgtcctg cagcacctgg tcggctttca tttctggcgc aaattccagc agagttacgt
    11221 gctcaacgat acccgccagg tcgattgccg cttccacgcc ggagttaccg ccgccgataa
    11281 ccgctacgcg tttgccttta aacagcgggc cgtcgcagtg cgggcagtag gtcacgcctt
    11341 tggtgcgata ctgatcttcg cccggaacgt tcatgttgcg ccattttgca ccggtcgcca
    11401 cgataatgct gcgtgctttc agtaccgcgc cagaagctgt ttcaatctgg tgtagaccac
    11461 cttcaaccgc tgccgggatc agtttgctgg cgctctggct gtcgatcaca tcaacgtcgt
    11521 attcatcaac gtgaactttc agtgcgcctg ccagcttctg cccttcagtc ttcggtacag
    11581 aaatgtagtt ttcgatatca acggtatcga ggatctgacc accaaaacgt tcgcccatca
    11641 gaccggtacg gatgccttta cgtgcggagt aaattgctgc cgctgcaccc gccgggccgg
    11701 aaccgacgat taatacgtca taagcatcac gcttgttcag ctcttctgcc gcacgttttt
    11761 ccgcgccagt atcaattttg gcaacgattt cagtcaacgt catgcggccc tgaccaaact
    11821 ctttcccgtt tacgaacact gccggaacgc ccatcacgtt gcgatcggtg atttcgttct
    11881 ggaaggtgcc gccgtcaatt gcagtgtgct tgatgcgcgg gttcagtacg ctcatcaggt
    11941 tcagcgcctg caccacgtcc gggcagttgt ggcaagagag cgagtaatag gtttcgaatt
    12001 caaaatcacc gtcaatatgg cgaatctgct ccagcagaga ctgcgcttct ttcgacggat
    12061 gaccaccggt ccacagcaac gccagtacca gcgaggtgaa ctcgtggccc agcggggagc
    12121 ctgcaaaacg tggcccctgg ttggaacctg ggttggtgat caggaaagac ggcttacgca
    12181 ccggcaagct gttatcttct ttaaaggtga ctttgtctga cagttctgcg atttcagcca
    12241 acagttcctt gatttctgcc gatttagcgc tgtcatccag cgtggcaatt aactcaacag
    12301 gcttggtcaa tttctcaagg taagccttga gttgagtttt catatttgtg tcgagcatgt
    12361 ttatctcctg ggctttaaac atcatcatgc aagcggcctc tgcggctacc tggatgcagc
    12421 ttgcatcatg gtgcggggaa gaaaatcggg tgccgcagca cccgattaat tacgcgattg
    12481 cggaacttag attttaccaa ccaggtccag agacggagcc agagttgctt caccttcttt
    12541 ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgtgctg ctttgatttt
    12601 acgcagcagg tcagacgcgt cacggccaat gccttcagcg gtaacttcga ttgcctggat
    12661 gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat cttcacgcat
    12721 gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat atttgatttt
    12781 agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat cggtagatac
    12841 tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa cgtcacccag
    12901 ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc tccagcggcc
    12961 ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct ggtttttaaa
    13021 aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt cgatgagatg
    13081 taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat cgataagttt
    13141 taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg cgataaaaag
    13201 taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt atcttcagcg
    13261 cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga ttacaacacc
    13321 ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg
    13381 ccttttactc gacttttgca ctgactgaaa aggacaaatt aatgttaaaa aagatacttt
    13441 tactggctct gcttcctgca atcgccttcg cagaggaact tccttcccca gtaaaagcga
    13501 ttgaaaaaca gggcattaca atcatcaaaa cgttcgatgc ccccggagga atgaaaggtt
    13561 atctcggaaa gtatcaggat atgggcgtca ccatctacct gactcccgat ggtaagcacg
    13621 ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca cttatcgaaa
    13681 aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa tcccactggc
    13741 tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt gttcgccgat ccgttctgcc
    13801 catattgtaa acagttctgg cagcaggcgc gcccgtgggt agattctggc aaagtgcaat
    13861 taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca gcggcaattc
    13921 ttgcctccaa agatcccgca aaaacctggc aagaatatga atcctctggt ggcaagctta
    13981 agctaaacgt gcctgcaaac gtaagtacag aacaaatgaa agtgttaagc gccaacgaga
    14041 aactcatgga cgatctgggg gcaaatgtca caccagcgat ctattacatg agtaaggaaa
    14101 atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc attatgggga
    14161 ataaataaaa gataattcat ggatggttga aatacatccc tgcttttcaa ccatcctaaa
    14221 aaagcatgaa ttgctcgctc tgccctaagt ggctatgcta ttttagaaaa acttaatcct
//
LOCUS       DAZFVO020000128        14219 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFVO020000128 DAZFVO020000000
VERSION     DAZFVO020000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 14219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.129"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(67..963)
                     /gene="yhaJ"
                     /locus_tag="ACHKNB_RS18280"
                     /old_locus_tag="pgaptmp_003656"
     CDS             complement(67..963)
                     /gene="yhaJ"
                     /locus_tag="ACHKNB_RS18280"
                     /old_locus_tag="pgaptmp_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HGU4197099.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1068..1769
                     /locus_tag="ACHKNB_RS18285"
                     /old_locus_tag="pgaptmp_003657"
     CDS             1068..1769
                     /locus_tag="ACHKNB_RS18285"
                     /old_locus_tag="pgaptmp_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HGU4197100.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1792..1956
                     /gene="yhaL"
                     /locus_tag="ACHKNB_RS18290"
                     /old_locus_tag="pgaptmp_003658"
     CDS             1792..1956
                     /gene="yhaL"
                     /locus_tag="ACHKNB_RS18290"
                     /old_locus_tag="pgaptmp_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HGU4197101.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2168..3478)
                     /gene="cyuA"
                     /locus_tag="ACHKNB_RS18295"
                     /old_locus_tag="pgaptmp_003659"
     CDS             complement(2168..3478)
                     /gene="cyuA"
                     /locus_tag="ACHKNB_RS18295"
                     /old_locus_tag="pgaptmp_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HGU4197102.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDSTAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWHGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3506..4837)
                     /locus_tag="ACHKNB_RS18300"
                     /old_locus_tag="pgaptmp_003660"
     CDS             complement(3506..4837)
                     /locus_tag="ACHKNB_RS18300"
                     /old_locus_tag="pgaptmp_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HGU4197103.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLRSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5112..6476)
                     /gene="tdcG"
                     /locus_tag="ACHKNB_RS18305"
                     /old_locus_tag="pgaptmp_003661"
     CDS             complement(5112..6476)
                     /gene="tdcG"
                     /locus_tag="ACHKNB_RS18305"
                     /old_locus_tag="pgaptmp_003661"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4197104.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HLVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6548..6937)
                     /locus_tag="ACHKNB_RS18310"
                     /old_locus_tag="pgaptmp_003662"
     CDS             complement(6548..6937)
                     /locus_tag="ACHKNB_RS18310"
                     /old_locus_tag="pgaptmp_003662"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HGU4197105.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6951..9245)
                     /gene="tdcE"
                     /locus_tag="ACHKNB_RS18315"
                     /old_locus_tag="pgaptmp_003663"
     CDS             complement(6951..9245)
                     /gene="tdcE"
                     /locus_tag="ACHKNB_RS18315"
                     /old_locus_tag="pgaptmp_003663"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HGU4197106.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9279..10487)
                     /gene="tdcD"
                     /locus_tag="ACHKNB_RS18320"
                     /old_locus_tag="pgaptmp_003664"
     CDS             complement(9279..10487)
                     /gene="tdcD"
                     /locus_tag="ACHKNB_RS18320"
                     /old_locus_tag="pgaptmp_003664"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HGU4197107.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10513..11844)
                     /gene="tdcC"
                     /locus_tag="ACHKNB_RS18325"
                     /old_locus_tag="pgaptmp_003665"
     CDS             complement(10513..11844)
                     /gene="tdcC"
                     /locus_tag="ACHKNB_RS18325"
                     /old_locus_tag="pgaptmp_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HGU4197108.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11866..12855)
                     /gene="tdcB"
                     /locus_tag="ACHKNB_RS18330"
                     /old_locus_tag="pgaptmp_003666"
     CDS             complement(11866..12855)
                     /gene="tdcB"
                     /locus_tag="ACHKNB_RS18330"
                     /old_locus_tag="pgaptmp_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HGU4197109.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12954..13892)
                     /gene="tdcA"
                     /locus_tag="ACHKNB_RS18335"
                     /old_locus_tag="pgaptmp_003667"
     CDS             complement(12954..13892)
                     /gene="tdcA"
                     /locus_tag="ACHKNB_RS18335"
                     /old_locus_tag="pgaptmp_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HGU4197110.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3727 a         3815 c         3313 g         3364 t
ORIGIN      
        1 gtttggttca aattaatctt agaattgggg cgacatttcg ccccttttta ttaataataa
       61 caaatattat tttccgctaa aaagtttggg aatttcccgc agacaccagg atttcgcttc
      121 ccccatactg tcacgacgcc aggccataat aatatcgatc tcgctggtcg attccgggct
      181 gacgacacgc aaccgccctt ccgcaatatc tttttcgacc atcggatacg gcatcgtcgc
      241 cacgccaagc cccgccagta atgcctgacg tttatcttca atcgtgctca ccgttaagcg
      301 cggctgtttg tccagcagct gtacggtcaa caccgggcgc tcacgagcgg tatccgctac
      361 cgcaattcca cgatatttca cgcgcgtcac ttcagataac ggttccggct cctgatgaat
      421 cgggtgatca ggcgcggcaa cgtagacgtt cattaaggta tagagcttgc gcgagttgat
      481 ctccgacgag gaacgaaaat gcatatccgg cgcgataacg atatccgccc gcccctgctc
      541 cagtcgttcc cacgcccccg ccagcacttc ggtgatgatt gccagttggg tattggcttt
      601 tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc ggtaccagcg cctcggtcac
      661 aatggtgaga tgcgtttccc aaccgcgcgc gagggcttcc gcatcggtag tcagtttatc
      721 tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc ctcccgacat tggtgaattt
      781 ggtgcgatgg cccgagcggt caaacagcac cacatccagc tcttcttcca gtttttgcat
      841 ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc gccgccgccg caaaactgcc
      901 ccggcgatcg atcgcatcca taacccgtag tgcttccagc gttaatgccc tttctttggc
      961 catttcgttc tcattcagga aatttgaaca taccgggcag aatatctggc taacaatgta
     1021 gcgtccagcc ccttaccata aaaggaagta aagagaggtc aagaattatg attactaccc
     1081 gaactgccag acagtgtgga caagcagact acggatggtt gcaggcccgg tatacttttt
     1141 cctttggaca ctacttcgac ccgaaattgt taggctatgc ctccctgcgc gtgcttaacc
     1201 aggaagtgct ggccccaggt gccgcctttc agccgcgaac ctatcccaaa gtcgatattt
     1261 taaatgtgat tctggatgga gaagcagagt atcgcgacag cgaaggcaat catgttcagg
     1321 ccagcgccgg tgaggcgttg ctgctctcta cccagccggg tgtcagctac agcgaacaca
     1381 atctcagcaa agacaaaccg ttaacacgaa tgcagctttg gctggacgcc tgcccgcagc
     1441 gagagaatcc gctgattcaa aaactggcgc ttaatatggg caagcagcaa ttaatcgcct
     1501 cgccagaggg tacgatggga agcctgcaat tacgccagca agtgtggctg caccatatcg
     1561 tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat
     1621 cgattcacgg gaaatttcat gcgcttacgc atcatgaaga gaaagcggcg ctgacctgcg
     1681 gtgatggggc gtttattcgt gacgaggcta acattacgct ggttgccgat tccccactgc
     1741 gcgctttgct gatagatttg cctgtctagt tgtttttaca gggagatgat gatgagtaaa
     1801 aaatcggcca aaaagcgcca gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc
     1861 gccaaaaatt ttggctatga agagatgttg agcgagctgg aagccatcgt cgcggatgct
     1921 gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac aggttgccgg atgcggcgaa
     1981 aacgccttat ccggcctaca aaatccaaca acattaacga attaaacaac ccgtaggccg
     2041 gataagatgc gccagcatcg catccggcat caggtacaaa acacaacaac atcaacaagt
     2101 tgagcacccc gtaggccgga taaggtgcgc cagcatcgca tccggcacaa tactcaaaac
     2161 ctggcgttta tctggccttg ctcgccataa tctcgataat ctgccgatcc gtttgctgca
     2221 tcgaatggct tgctaacgca cacaggttgg caatcgactg ctcaacatca tgcgccacga
     2281 tcccttcatt gccggtcacg gcggtatcat ccagcgccat taacaccgct ttccacgcag
     2341 ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc
     2401 cgctgacatc gccgatcata ctgctgatcg ccatcgagat ggtttcataa cgcccatcca
     2461 ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca cacagcgcag
     2521 acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac
     2581 gcgccagccg ttcatcatcc gctccgaagt gttctgctac caccaccaca ggcattgttg
     2641 cggtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca
     2701 tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca
     2761 gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc
     2821 tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg
     2881 ccgcaaacgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca
     2941 ccgtcagcgg agactcttgc tcgccctctg ccacacacgc ctgctgggta aacaccacac
     3001 cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc
     3061 acttctcacc gtgccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga
     3121 tcttaacgga gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag
     3181 ttgagtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg
     3241 ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gacgcccaga ccgttcttca
     3301 tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag
     3361 caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt
     3421 cctcctgaac tgcaaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat
     3481 taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacggagaa acacaaagca
     3541 acaaaccagt gacgataatc aggtacagag acattccttt gtatttgtgc aatgccggta
     3601 ctttgtaaac cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga
     3661 tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg
     3721 cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc
     3781 gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa
     3841 agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc acccacgctg
     3901 cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcatagg
     3961 cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg
     4021 tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt
     4081 caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca
     4141 gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg
     4201 gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg cccagcgccg
     4261 cgaccaccag cagcttggtc agcaccatgc cggtcgaaat tttgaataac aatttctcgc
     4321 cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg
     4381 accgcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg
     4441 cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga
     4501 tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg
     4561 attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca
     4621 ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa
     4681 tccccgcgcc aatcgccatc ccgatactca taatcaccca gccggtgtca gtactgtcga
     4741 atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag
     4801 cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca atattttgta
     4861 gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc
     4921 gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac
     4981 tttgaaataa cggagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat
     5041 tctctatttg cggcagatca caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc
     5101 tgacagcaat gtcagccgca gaccacttta atggccagtc ctccgcgtga tgtttcgcgg
     5161 tatttatcgt tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa
     5221 acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg cgtttactgc tttcacggca
     5281 ttaatggcat tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc
     5341 agcccaaggt tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg
     5401 cccagtagtt cagttaaccc tgccgccgcc attgaacagg ccacgccaat ctccccctga
     5461 cagccgacct ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aatagccccc
     5521 gctgccagaa aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa
     5581 taagccagca ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc
     5641 ccgccagctg cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca
     5701 ttcatcggat cgttagagat gttatcgcta gaaaccagct gacgacgcag cgctacggca
     5761 cgacgcggca cattgagcgg accaggcagc acgccttcag tgttcatccc gcgttcaata
     5821 ccgtcatgca tcacttgcca gatacgggta aaccctgcgt caatttccgc tttactgcgc
     5881 agcgctagct cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt
     5941 ttcagcagtt cacctgctga gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac
     6001 aggccgaagt gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttactt
     6061 aatagcgctt cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac
     6121 atttcgggat ggaagataat gttctttgct acaggaaaat caacaagatg cgcaccagat
     6181 gccactggca gccgcccgct gcgcgttact aactctataa atgcagggat ctcatcaatg
     6241 acaacatcct gcggactgtt tcctgccagt cccatgataa tggcgacatc cgtggcatgg
     6301 cctttgcccg tcagtgacaa cgacccatac agatcgacca caatatggct cgtcgcggtt
     6361 aataagccgc tactttccag ccgatcaata aaactttttc cggcattcat tggccccacg
     6421 gtatgcgaac tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc
     6481 acaccctcgg attgccgttc agtgaagtgg agcggaacga ccttgcgacc gtcccgctca
     6541 cgaggcttta cgcactacgt actgcgatag cttcaatttc cagcttcaca tctttcggca
     6601 aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact
     6661 gcttatagac ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct
     6721 tgatgatatc gcccacgctc agcccggcag caaccacgat cgctttgacg ttttcgaggc
     6781 ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc ggtctgcggg caaaccggaa
     6841 tttgcccgga ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg
     6901 cgcctggggc acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc
     6961 tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc
     7021 acggcgtagc cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca
     7081 tccagcagca tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc
     7141 gcttcgtggt ggaaataccc atccagcagg ccgacaaggt tggttttacg tactggatct
     7201 tctttgccca gcgccgcagg aacgattgag aaggtgtacg agatcccatc tttggcgtag
     7261 gtgaacggca gtttcgccac cgacgtcaat gaggccacgg cacctttgcg gtcacgacca
     7321 tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta
     7381 ccggttttct ggccgtacac cacgttagaa gtgatagtca gaatcgactg ggtagggacg
     7441 gcgttgcgat aggttggcag cgctttaatt ttcttcataa agcgttcaac caggtcgcag
     7501 gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc gtcgatttca
     7561 aagtccaccg ctaggccgtt ttcgtcacgg attggtttca cgcgggcata tttgatggca
     7621 gacagggagt ccgtcgccac cgacaggccc gcgatgccgc atgccatagt gcgatagaca
     7681 tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga
     7741 atgatattca gcgcgctgat gtactgcacc gccagccagt ccatgaagtg atcgaggctg
     7801 tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc
     7861 tgaatcttca gcttctcgtc caccccgccg ttaattgcgt agagcagcgt tttcgccagg
     7921 ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca ccattgggct gacgcagcag
     7981 gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca gatcgtcatt ttcatactgc
     8041 aaggaagagg tgacgatcga cacctgcgcg gcatattttt tgaaggcaat cggtaattct
     8101 tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc
     8161 aaatagcgga aggagttttt ggtcaccagc gtacgaccgt ccagccccat cccgccgatc
     8221 acttccgtcg cccagattgg gtcgccggag aacagcgaat caaattccgg tgtacgcagg
     8281 aagcgtacca tacggatctt catgatgaag tgatcgatca gttcctgtgc ctgctgctca
     8341 ttgagtacgc cagctttaaa gtcgcgctca atgtagatat cgaggaacga tgccgtgcgg
     8401 cccagcgaca tcgcgccgcc attttgcgat ttcactgccg ccagataagc gaagtagagc
     8461 cactgcaccg cttcctgcgc attctgcgcc gggcgagaga tatcaaagcc atatttcgcc
     8521 gccatttcct gaatctgcaa cagcgcatga cgatgctctg ccagctcctc acgcagacgg
     8581 atggtggctt ccagatcctc gcctttttcc agacgagact ggagatcggc aaattgcagt
     8641 tcgcgttcac gtaccagata actgatgcca tacagcgcta cgcggcgata gtcaccgata
     8701 atgcgcccac ggccatagcc atctggtaaa ccggtcagca cgccagattt acggcagcgc
     8761 agcatatccg gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta
     8821 aacagatatt caaattcact gtccatttca cggccatagg cgtggaatga acttttaatc
     8881 atattaatgc caccgaacgg gtgtagcgca cgtttcaacg gcgcatccgt ttgcaggcca
     8941 acaatttttt ccagcggctg gttaatatat cccgcatcat gagcggtaat tgtggtggca
     9001 atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat accttccatt
     9061 actttttccc acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga ttcatcgcct
     9121 tcatacggtg tatagttatg ttgaataaaa tcgcggacat taatttcgtt tttccagtcc
     9181 gtacctttaa agccaagcca tgcgtcggcg tacagcttat cgctggtatc aatatctacc
     9241 ttcatgaaaa ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg
     9301 ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaata
     9361 acagcgcaaa tgacacgcgc attttcactg gaaacaattc gctcaccaca ggagttagag
     9421 cgattattca tttctgtatc aatctctacg cctaatacag ccaaatgttc catgaccaga
     9481 cgacgaatta agcttgaatt ctctcctatt ccgccggtga atataattcc atccaggcga
     9541 cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt
     9601 gccagttgcg cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa
     9661 gaaagaccgg aaatacctaa taatcccgac tctttattca ctacgcgttc caggtcaccc
     9721 aggctctggt tggtttggct ggcgacccag gacatcgcac caaagtcgac atcgccactg
     9781 cgggtaccca tcatcaagcc ttccagcggc gtcattccca ttgaagtatc aacactctga
     9841 ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag
     9901 tcatcttccg ccagattcag cagcgaatgt gcgcgctggg aaacatagcg gtgcgacgtg
     9961 ccgtggaaac cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat
    10021 aaataagctt ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt
    10081 acgcccggaa ataattgctg cgccgattca ataccactta aattggcgta attatgcagg
    10141 ggtgccagtg gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac
    10201 tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa ttaaggccac actgtcattt
    10261 aaattccgtt tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga
    10321 gccagcggtg ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg
    10381 tcggcaatac ctgacattaa tacttcacag tcgctggcat cgagtacgga aaacttaatc
    10441 gaagacgaac cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc
    10501 ctgagttacg gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac
    10561 ggtaacaaac acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat
    10621 ggcatacatc ggcaacaggc acagcaggga tgcgataatc ggtgcgccca tggcttcaat
    10681 aaggtcgagg atgttcgggt tggcgtaggc aacaacccag gtggagccca tgatgaagat
    10741 catgctgata gtgttcagtt tacccagcga cactttagtt ttgtcgcctt tataaccgaa
    10801 cttcagaatc agaccattca agccttccag cgtccccaga tagtgaccga agaaagattt
    10861 gaagatagcc acgagtgcga tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt
    10921 tttggtaccg gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg
    10981 cgctttggct tccgccatgt tggccggaga cagcgtaaac aggcagctaa aggcaaagaa
    11041 catcaccact gcaaccatca gcatgctggc acgagaaatg atttgggaac atttacgttc
    11101 ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt ttagaaacca cgaaggaaga
    11161 gacgattggc gagaagttaa aggagaaaac catgatggaa atccccagcc agacagtgat
    11221 caggataccg tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt cgataactgc
    11281 agagttccag taagggatca gcgacagaga aatcagcacc aggctggcga taaacggcca
    11341 taccaggtag ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat
    11401 cagcagcaac aggaacagcg ccacaaagcc gcgattcagc ggtgcaaatc cgagctggtt
    11461 ttcccagaac gtcataaagg tattggtaat agtaacgcca taaatccaca gcagtgggca
    11521 aatcgcgaag aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc
    11581 caccgtttcc gtaatgttgc cggaagggtt agagccagaa agacacagac gcgccagcgc
    11641 ccggtggcaa taaaatgcga tggggtatgc caataccaac atcagaagaa tcgggatcag
    11701 tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa
    11761 caagcctaac gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga
    11821 tacaatgcta tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa
    11881 accggtgatt tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt
    11941 ttttctgttt tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc
    12001 gcctgcgcct tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat
    12061 ttcgtcttcg ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa
    12121 attacccggg cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt
    12181 tatttctccg gagtggaaag aagccgccat gccgtgaacg ttttctgact gtacgccaat
    12241 aacacgaatg gtcgggttaa tagatttaat tgccaccgca ataccagcaa ttaaaccacc
    12301 accaccaatt ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc
    12361 aatcgttccc tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacgacc
    12421 ttccatttcg acaatttcgc tcactttagc gatagtgtcg ttgaaattat caccatgcag
    12481 aacgacttct gcggagtagt cgcacgttgc cgctactttg gattttggcg caccttttgg
    12541 catcaccact ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg
    12601 gttgcccgca gaacaggcca ctacaccttt acgtttttcc gcatcagtca gtgaacttaa
    12661 tttattaaat gcgccacgaa ttttaaatga acccgtacgc tgcatatttt caaacttcag
    12721 gaatatttca cctttgcaac gttcactaaa atagtttgag cgaggcatgc ctgttttata
    12781 aattcgccca gccagtcgtt gtttcgcttc aataatgtca tcaatagcaa ccggcagatc
    12841 gtatgtaata tgcattataa aacctcttcg cctgaattaa atatgtaggt aaccgtaacc
    12901 gacacctgca aaacagacag gtggattatt tatgtttaaa taaaaacaaa taactaacca
    12961 acttctatta attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc
    13021 accaaaaccg atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt
    13081 gccacaggta atgtttcttc aaccggaata gtaataaatt gatttgaacc aaaaggtgac
    13141 gtcatatcac aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc
    13201 acgactgagt cggttttaac gatgttttca atactgatgc catttctttg taacgtagta
    13261 agaagctcgc tgtagtaccc catattggtt tgtggcaaca cccactgttc gttcttcaac
    13321 gactccagcg tggtggtgcc ggtgcatgtt cgggacttac tggctaccaa cacaaactcg
    13381 gactcgaaca gcggctcaac atgtaaatcc tgaagcttca tttctgcact taacgtacca
    13441 atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca
    13501 tacatagaaa cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata
    13561 aaagtaaaac caatcaatga aggaaaacca aatgagactt ccaccaccgc ctcagaagac
    13621 ataccgctta tctcattaac catatttttc atttcacggg taatggattc ggaacgggag
    13681 agtaacaatt gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaattcc
    13741 acaccaaaat aatcttcaat atcgttaatg attttactga cggctggttg agttaaccct
    13801 aattcttttg cagccgagcc gatagagcca cttctaatga cttcctgaaa gactaccagg
    13861 tgctgcgttt tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac
    13921 ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac aaattaactt
    13981 tcaatacttt ccaaggtatc gttattacaa ttaaacacct ttaaatatca acaagttaaa
    14041 gcataaaaat cagcataaac cctgattttt gtcaaaaaaa atgacgggga taaccatatt
    14101 ttatggcgat aacatcattc gttatgtggt taatacaaaa aaggggctga gaccatattt
    14161 caagcaatta ccggataatt accaggcaaa atttgagtta aatttaatga gcaagttct
//
LOCUS       DAZFVO020000129        13728 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFVO020000129 DAZFVO020000000
VERSION     DAZFVO020000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.130"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            377..1090
                     /gene="aphA"
                     /locus_tag="ACHKNB_RS18340"
                     /old_locus_tag="pgaptmp_003668"
     CDS             377..1090
                     /gene="aphA"
                     /locus_tag="ACHKNB_RS18340"
                     /old_locus_tag="pgaptmp_003668"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HGU4197111.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1201..1617
                     /gene="yjbQ"
                     /locus_tag="ACHKNB_RS18345"
                     /old_locus_tag="pgaptmp_003669"
     CDS             1201..1617
                     /gene="yjbQ"
                     /locus_tag="ACHKNB_RS18345"
                     /old_locus_tag="pgaptmp_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HGU4197112.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1621..1977
                     /gene="yjbR"
                     /locus_tag="ACHKNB_RS18350"
                     /old_locus_tag="pgaptmp_003670"
     CDS             1621..1977
                     /gene="yjbR"
                     /locus_tag="ACHKNB_RS18350"
                     /old_locus_tag="pgaptmp_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGU4197113.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2012..4834)
                     /gene="uvrA"
                     /locus_tag="ACHKNB_RS18355"
                     /old_locus_tag="pgaptmp_003671"
     CDS             complement(2012..4834)
                     /gene="uvrA"
                     /locus_tag="ACHKNB_RS18355"
                     /old_locus_tag="pgaptmp_003671"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGU4197114.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5088..5624
                     /gene="ssb1"
                     /locus_tag="ACHKNB_RS18360"
                     /old_locus_tag="pgaptmp_003672"
     CDS             5088..5624
                     /gene="ssb1"
                     /locus_tag="ACHKNB_RS18360"
                     /old_locus_tag="pgaptmp_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HGU4197115.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5723..6004)
                     /gene="yjcB"
                     /locus_tag="ACHKNB_RS18365"
                     /old_locus_tag="pgaptmp_003673"
     CDS             complement(5723..6004)
                     /gene="yjcB"
                     /locus_tag="ACHKNB_RS18365"
                     /old_locus_tag="pgaptmp_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HGU4197116.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6434..8020
                     /gene="pdeC"
                     /locus_tag="ACHKNB_RS18370"
                     /old_locus_tag="pgaptmp_003674"
     CDS             6434..8020
                     /gene="pdeC"
                     /locus_tag="ACHKNB_RS18370"
                     /old_locus_tag="pgaptmp_003674"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HGU4197117.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8023..8346)
                     /gene="soxS"
                     /locus_tag="ACHKNB_RS18375"
                     /old_locus_tag="pgaptmp_003675"
     CDS             complement(8023..8346)
                     /gene="soxS"
                     /locus_tag="ACHKNB_RS18375"
                     /old_locus_tag="pgaptmp_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HGU4197118.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8432..8896
                     /gene="soxR"
                     /locus_tag="ACHKNB_RS18380"
                     /old_locus_tag="pgaptmp_003676"
     CDS             8432..8896
                     /gene="soxR"
                     /locus_tag="ACHKNB_RS18380"
                     /old_locus_tag="pgaptmp_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HGU4197119.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9442..10791
                     /gene="ghxP"
                     /locus_tag="ACHKNB_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /gene_synonym="yjcD"
     CDS             9442..10791
                     /gene="ghxP"
                     /locus_tag="ACHKNB_RS18385"
                     /old_locus_tag="pgaptmp_003677"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HGU4197120.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10943..12592
                     /gene="yjcE"
                     /locus_tag="ACHKNB_RS18390"
                     /old_locus_tag="pgaptmp_003678"
     CDS             10943..12592
                     /gene="yjcE"
                     /locus_tag="ACHKNB_RS18390"
                     /old_locus_tag="pgaptmp_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HGU4197121.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12746..13728)
                     /locus_tag="ACHKNB_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /pseudo
     CDS             complement(12746..13728)
                     /locus_tag="ACHKNB_RS18395"
                     /old_locus_tag="pgaptmp_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         3275 a         3306 c         3532 g         3615 t
ORIGIN      
        1 aaattagtca ccattgtagg atagggttct ggtcaatcag gaatcatctt tataaacttc
       61 gattgttttt gtaatgctgt atcattaagt tcattaaatc gtacagcaga taaatgttct
      121 atcaaatttc gctcatttgc agaggattca tcataataaa cgtaaaatta atgtatcctt
      181 acatcgagta ataaacattt tttatacaaa aaaagacagg aacgtattta ctgggttaaa
      241 cataatcatc ctgcttttca tcacaaaaac cgcagataat ccttcctttc cccggcagct
      301 ggcgttatgg tcagatggtt tttgcaacaa atctcacaat aaaaagtttc aacatactga
      361 ctatttaggg aaaaatatgc gcaagatcac acaggcaatc agtgccgttt gcttattgtt
      421 cgctctaaac agttccgctg ttgccctggc ctcatctcct tcaccgctta accctgggac
      481 taacgttgcc aggcttgctg aacaggcacc aattcattgg gtttcggtcg cacaaattga
      541 aaatagcctc gcagggcgtc cgccaatggc ggtggggttt gatatcgatg acacggtact
      601 tttttccagc ccgggcttct ggcgcggcaa aaaaaccttc tcaccagaaa gcgaagatta
      661 tctgaaaaat cctgtgttct gggaaaaaat gaacaatggc tgggatgaat tcagcattcc
      721 aaaagaggtc gctcgccagc tgattgatat gcatgtacgc cgcggtgacg cgatcttctt
      781 tgtgactggt cgtagcccga cgaaaacaga aacggtttca aaaacgctgg cggataattt
      841 tcatattcct gtcaccaaca tgaatccggt gatctttgcg ggcgataaac cagggcaaaa
      901 tacaaaatcg caatggctgc aggataaaaa tatccgaatt ttttatggcg attccgataa
      961 cgatattacc gccgcacgcg atgtcggcgc tcgtggtatc cgtattctgc gcgcctccaa
     1021 ctctacctac aaacccttgc cacaagcggg tgcgtttggt gaagaggtga tcgtcaattc
     1081 agaatactga cagagcggga gagcgtgacg ctctcccgaa tgctgttttt ttaatcacac
     1141 ctttatcctt tcgctgtctt gctgcaaact gattaagaga gttttatcaa ggagcagcac
     1201 atgtggtatc aaaagacgct cacgcttagc gccaaatctc gtgggtttca tctggtaacg
     1261 gatgaaattc tgaatcagct ggctgatatg ccgcgcgtta acatcggctt actgcatctg
     1321 ttgctgcaac atacctccgc ctctctgaca cttaatgaga actgcgatcc caccgtacgc
     1381 cacgacatgg agcgtttttt cctccgcacc gttcccgaca acggaaatta tgagcatgac
     1441 tatgagggag cagacgatat gccttctcat atcaaatcct caatgctggg aacatcgctt
     1501 gtattgccgg tacataaagg gcgtattcag accggcacct ggcaaggcat ctggctgggg
     1561 gaacatcgca tccacggcgg atcgcgtcgc atcatcgcga cactacaagg ggagtaaaaa
     1621 atgaccattt cggagttgct acaatattgc atggcaaaac caggcgcaga acagagcgtg
     1681 cataacgact ggaaagcgac gcagatcaaa gtggaagatg tactgtttgc gatggtgaaa
     1741 gaagtagaaa atcgcccagc tgtttcgctg aaaaccagcc cggagctggc ggagctgcta
     1801 cgtcagcagc acagcgatgt gcgtcctagc cgccatctga ataaagcgca ctggagcacc
     1861 gtgtatctcg acggttcgct gccggattcg caaatctatt atctggtgga tgcgtcttat
     1921 cagcaggcag tgaatttact accggaagaa aagcgtaaat tgctggtaca actctgaaag
     1981 gaaaaggccg ctcagaaagc ggccttaacg attacagcat cggcttgagg aagcgtgccg
     2041 tatgcgaagc ttcgcactcc gcgacggttt ctggcgtacc ggagacgagg atttcgccgc
     2101 cgccactgcc gccttctggt cccaggtcga caatccagtc agcggttttg atcacgtcga
     2161 gattgtgctc aatcaccaca atggtattgc cctgatcgcg cagtttatgc agcacgtcga
     2221 ggagttgctg aatatcggcg aagtgcagac cggtggtcgg ctcgtcgaga atatacagcg
     2281 tctgcccggt gccgcgtttt gacagctcac gcgccagctt cacgcgctgg gcttcaccac
     2341 cagaaagtgt ggttgcggac tgccccaggc gaatgtacgt cagaccaacg tccatcaacg
     2401 tttgcagctt acgcgccaga gctggcaccg catcaaagaa ctcacgcgcc tcttcgatgg
     2461 tcatatccag cacttcgtgg atggttttgc ctttgtactt aatttccagc gtttcacggt
     2521 tatagcgttt acctttgcac tgatcgcacg gcacgtagat atccggcagg aagtgcatct
     2581 ccactttgat cacaccgtcg ccctgacagg cttcgcagcg cccgccacgg acgttaaagc
     2641 tgaaacgtcc tggcgtataa ccacgcgcac gggattccgg tacgcccgca aacagttcgc
     2701 gcacaggtgt aaacacgccg gtataggtcg ccgggttaga acgtggagta cgaccaattg
     2761 ggctttggtc gatatcgatc actttatcga aatgctccag cccctgaata tcgcgatacg
     2821 gtgccggttc ggcgatggtc gcaccattca actggcgttg ggcaatcggg aacagtgtgt
     2881 cgttaatcag cgtcgattta ccggaacctg aaacccctgt gatgcaggta aacagaccga
     2941 ctggcagcgt gagcgtcacg tccttcaggt tgttgccgcg tgcgcctgtc agcttcagca
     3001 ctttttccgg atttgccgga acgcgtttct tcggcacttc aatcttgcgt ttaccgctca
     3061 tgtactgccc ggtcaacgac tccggcaccg ccataatcgc ttccagcgga ccttctgcga
     3121 ccacttcacc gccgtgaaca cctgcgcccg ggccaatgtc gatcacatgg tcagcggcgc
     3181 gaattgcgtc ttcgtcgtgc tccaccacaa tcacggtatt accgagatcg cgcagatgga
     3241 taagcgtacc caacaggcgc tcgttatcgc gctggtgcag gccgatagat ggctcgtcca
     3301 gcacgtacat aacgccaacc aggcccgcac caatctggct cgccagacgg atacgctggg
     3361 cttcaccgcc ggaaagcgtt tctgccgagc gggaaagcgt caggtaattc aggccgacgt
     3421 taacgaggaa tttcaaacga tcgccgatct ctttaaggat tttttccgca atcttcgccc
     3481 gttgaccagc gagtttgaga ttgttgaaga attccatcgc atgaccgatg ctcatgtcgg
     3541 agatagcagg cagcggcgta ttctcgacat aaacgtggcg cgcttcccga cgcagacgcg
     3601 tcccttcgca gctggcgcat gggcgattgc tgataaactt ggctaattct tcacgtaccg
     3661 cactggattc tgtctcttta taacggcgct ccatattgtg cagcacgcct tcgaacggat
     3721 gacgacggat ggaggtatcg ccacgatcgt tcatgtattt gaattcaatg ttttctttgc
     3781 cagaaccgta caacaccact ttatgcacgt tcgcgctcag gctgccccac ggcgcttcga
     3841 cgtcgaactt atagtgatct gccagcgatt tcagcatctg gaagtaatag aagttgcggc
     3901 gatcccagcc acggatcgca ccgccagcca gtgacagctc ggggttttgg atcacgcggt
     3961 caggatcgaa atattgctgt acgccaaggc cgtcacaggt tgggcaggca cctgccgggt
     4021 tgttaaacga aaacagtcgc ggctccagtt cacgcatact gtagccgcaa attgggcagg
     4081 cgaagttggc ggagaacagc agctcttccg ctttcgggtc gtccatatcc gccactaccg
     4141 ctgtaccacc ggaaagctcc agcgcggttt caaacgactc cgcaagacgt tgggtaagat
     4201 cgtcacgcac cttgaagcga tcaaccacca cttcaatggt atgtttcttt tgcagttcca
     4261 gtttcggcgg atcggaaaga tcgcagactt cgccatcaat acgagcacgg atgtaaccct
     4321 ggcttgccag gttctccagc gttttggtgt gttcgccttt gcgctcttta atgattggcg
     4381 cgagcagcat cagacgcttg ccttccggct gcgacagcac gttatccacc atctggctga
     4441 cggtttgcgc cgccagcggg acgtcgtggt ccggacagcg cggttcgccg acgcgggcga
     4501 acaacaaacg caaatagtcg tggatttcgg tgattgtccc caccgtagaa cgcgggttat
     4561 gagacgtcga tttctgctca attgagatgg caggagaaag cccctcaata tgatcgacgt
     4621 ccggcttttc catcagtgac agaaactgcc gcgcgtaggc ggaaagggat tcaacgtaac
     4681 ggcgctgccc ttcggcatat aaggtgtcga aagcgagcga ggatttgcca gaacccgaaa
     4741 gcccggtcac gacaatgagc ttgtcgcggg ggataacgag gttgatgttt ttgagattat
     4801 gggtgcgggc gccccgaact tcgatcttat ccattcacct ttcccggatt aaacgctttt
     4861 ttgcccggtg gcatggtgct accggcgatc acaaacggtt aattatgaca caaattgacc
     4921 tgaatgaata tacagtattg gaatgcatta cccggagtgt tgtgtaacaa tgtctggcca
     4981 ggtttgtttc ccggaaccga ggtcacaaca tagtaaaagc gctattggta atggtacaat
     5041 cgcgcgttta cacttattca gaacgatttt tttcaggaga cacgaacatg gccagcagag
     5101 gcgtaaacaa ggttattctc gttggtaatc tgggtcagga cccggaagta cgctacatgc
     5161 caaatggtgg cgcagttgcc aacattacgc tggctacttc cgaatcctgg cgtgataaag
     5221 cgaccggcga gatgaaagaa cagactgaat ggcaccgcgt tgtgctgttc ggcaaactgg
     5281 cagaagtggc cagcgaatat ttgcgtaaag gttctcaggt ttatatcgaa ggtcagctgc
     5341 gtacccgtaa atggaccgat caatccggtc aggatcgcta caccacagaa gtcgtggtga
     5401 acgttggcgg caccatgcag atgctgggtg gtcgtcaggg tggtggcgct ccggcaggtg
     5461 gtaatatcgg tggtggtcag ccgcagggcg gttggggtca gcctcagcag ccgcagggtg
     5521 gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca gtccgctccg gcagcgccgt
     5581 ctaacgagcc gccgatggac tttgatgatg acattccgtt ctgatttgtc attaaaacaa
     5641 taggttatat tgttttaagg tggatgatta aagcatctgc cagccataaa aaagaagcct
     5701 ccgttatgga ggcttctacg tatcaggtca aaatcattgg ccattgtggc ggtgtctgat
     5761 tcatctcttc tatcatcacc gactcgatgt tgtgccagat agcggtgatg tccgccattg
     5821 tgatgccaag caaccccatt gcgaaccaac aggcggaaac aacgcccaga ccgctgctga
     5881 tcaccgcaag cccgacataa tcagaccgac gaaaacggat gttgagtgtg ctggccagaa
     5941 acatcattac ggcactaaga agttgccagc gaagaagaac cacgccagtg gtgagggtag
     6001 ccatcaaaca attcctctga aaagagccga tgccctggac agcgcggcgt tgttcacggg
     6061 aggcgtggta cactctggct atcgcggggc ttgcagaaca caaaaatgaa acactctgtt
     6121 tgtttcatta attttgtgaa ctatatcaca attgattgtt tgttagccat attaggccgt
     6181 gacttttatt gctgtacaga ttatgtggtt tttcagtggc attaagggca tatcttcccg
     6241 ccgcctctgc attcctgtag gaaattaatt ttgaatatca atgaattatt ttcatccagg
     6301 tgacgattag aaaggtatca atttcaaatc aggcaaaagt gctatttata ccgtaagatt
     6361 tatctaaaga cgtcggtacc tagggttttc accttgcaat ggccgggtat aaacaggcag
     6421 gaaattgata gcaatgagtc atcgtgcacg acaccaatta ctggcgttgc cgggcattat
     6481 ctttttagtt ctctttccca tcattctttc gctatggatt gccttccttt gggcaaaatc
     6541 agaagtgaat aatcagctcc gaacctttgc tcaactggca ctggataaat ccgagctggt
     6601 cattcgccag gcagatttag tgagcgatgc agctgaacgc tatcaggggc aagtttgcac
     6661 tccagcccat caaaagcgaa tgttgaatat tattcgtggc tatctttata ttaatgaatt
     6721 gatctatgcc cgtgataacc attttttatg ctcatcgctg atagcgcctg taaacggcta
     6781 tacgattgca ccggccgatt ataagcgtga acctaacgtt tctatctatt attaccgcga
     6841 tacgcctttt ttctctggct ataaaatgac ctatatgcag cggggaaatt atgtggcggt
     6901 tatcaaccct ctcttctgga gtgaagtgat gtctgatgac ccgacattgc aatggggtgt
     6961 gtatgatacg gtgacgaaaa cctttttctc gttaagcaaa gaggcctcgg cagcaacgtt
     7021 ttctccgctg attcatttga aggatttaac cgtacaaaga aatggctatt tatatgcgac
     7081 agtttattcg acaaaacgcc caattgcagc cattgttgcg acttcatatc aacgtcttat
     7141 aacccatttt tataatcatc ttatttttgc gttgcccgcc ggtattttgg ggagtcttgt
     7201 tctgctatta ctctggctac gtattcgaca aaactattta tctcccaaac gtaaattgca
     7261 acgcgccctc gaaaaacatc aactttgtct ttattaccag ccaataatcg atatcaaaac
     7321 agaaaaatgt atcggcgctg aagcgttgtt acgttggctt ggtgagcagg ggcaaataat
     7381 gaatccggca gagtttattc cgctggcaga aaaggagggg atgatagaac agataactga
     7441 ttatgttatt gataatgtct tccgcgatct gggcgattac ctggcaacac atgcagatcg
     7501 ctatgtttct attaacctgt cggcctccga ttttcatacg tcacggttga tagcgcgaat
     7561 caatcagaaa acagagcaat acgcggtgcg tccgcagcaa attaaatttg aagtgactga
     7621 acatgcattt cttgatgttg acaaaatgac gccgattatt ctggctttcc gccaggcagg
     7681 ttacgaagtg gcaattgatg attttggtat tggctactct aacttgcata accttaaatc
     7741 attgaatgtc gatattttga aaatcgataa atcgtttgtt gaaacgctga ccacccacaa
     7801 aaccagtcat ttgattgcgg aacacatcat cgagctggcg cacagcctgg ggttaaaaac
     7861 gatcgctgaa ggcgtcgaaa ctgaggagca ggttaactgg ctgcgcaaac gcggcgtgcg
     7921 ctattgccag ggatggtttt ttgcgaaggc gatgccgccg caggtgttta tgcaatggat
     7981 ggagcaatta cccgcgcggg agttaacgcg cgggcaataa aactacaggc ggtggcgata
     8041 atcgctggga gtgcgatcaa actgccgacg gaaaacgcgg gagaaggtct gctgcgagac
     8101 ataacccagg tccattgcga tatcaaaaat cggacgctcg gtggtgcgca actcaacggc
     8161 ggccagtaac aggcgacgtt ggcgaatgta atcgccaagc gtctgatgcg tcaccgtgcg
     8221 gaacattcgt tgcaagtacc actttgaata gcctgatttt tttgcgacta catcaatgtt
     8281 aagcggctgg tcaatatgct cgtcaatcca tgcgataaga tcctgaataa ttttctgatg
     8341 ggacataaat ctgcctcttt tcagtgttca gttcgttaat tcatctgttg gggagtataa
     8401 ttcctcaagt taacttgagg taaagcgatt tatggaaaag aaattacccc gcattaaagc
     8461 gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg gcggtatcgg cgctgcattt
     8521 ctatgaaagt aaagggttga ttaccagtat ccgtaacagc ggcaatcagc ggcgatataa
     8581 acgtgatgtg ttgcgatatg ttgcaattat caaaattgct cagcgtattg gcattccgct
     8641 ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg catacgttaa gtgcgaaaga
     8701 gtggaaacag ctttcgtccc aatggcgaga agagttggat cggcgcattc ataccttagt
     8761 ggcgctgcgt gatgaactgg acggatgtat tggttgtggc tgcctttcgc gcagtgattg
     8821 cccgttgcgt aacccgggcg accgcttagg agaagaaggt accggcgcac gcttgctgga
     8881 agatgaacaa aactaaagcg ccacaagggc gctttagttt gttttccggt ctttgtcttt
     8941 ctctctatcc cgctggtaca caggagggtt tcccccgacg tcaacacacc tcattcgagc
     9001 acgtggtgga ggttccggtt ggtgttgatg ctttaattgt atgtcaccga cgtttcttcg
     9061 ccagtgtaaa agtatacttt ttaaccgcaa tatttttgtc atctcagacg attttttatc
     9121 gcaatcctga acggtatacg gctcgataac gctgcaatct tgcgcaccga cgataacgtt
     9181 tgcgcatcaa ttgcctgttt tttcatcgtc aagacaataa aagagaaaaa agcagcaaac
     9241 ttcggttgaa aaagccgcta tgatcgccgg ataatcgttt gcttttttta ccacccgttt
     9301 tgtatgcgcg gagctaaacg tttgcttttt tgcgacgcag caaattgtcg caaacctgga
     9361 gcaggaagat aacgtttcgc tggcagggga ttgtccgcca cgcatcttga cgaaaattaa
     9421 actctcaggg gatgttttct tatgtctacg ccatcagcgc gtaccggcgg ttcactcgac
     9481 gcctggttta aaatttcaca acgtggaagc actgtccgtc aggaagtggt tgccgggtta
     9541 acaacgtttc tggcgatggt ctactcggtc atcgtcgttc caggtatgtt gggtaaagca
     9601 ggcttcccgc ctgcggcagt tttcgttgca acctgtctgg ttgccggact cggttctatc
     9661 gtgatgggtc tgtgggctaa tctgccgttg gcgattggtt gcgccatctc cctgacggcg
     9721 tttaccgcat tcagcctggt gctggggcaa catattagcg tacctgtcgc gctgggtgcc
     9781 gtgttcctga tgggtgtgct gtttacggta atttctgcca cgggtatccg tagctggatt
     9841 ttgcgcaact tgcctcacgg tgtggcgcac ggcacgggga tcggtatcgg tctgttcctg
     9901 ctgctcattg ccgctaatgg tgtcggtctg gtgattaaaa acccgcttga tggtctgccc
     9961 gttgcgctgg gtgatttcgc gaccttcccg gtgattatgt cactggtagg tctggcggtg
    10021 atcatcggcc tggaaaaact gaaagtccct ggtggcattc tgctgaccat tatcggtatc
    10081 tcaattgtcg gtttgatctt cgatcctaac gtccatttct ccggcgtttt cgccatgcct
    10141 tcattgagcg atgaaaacgg caattcactg attggcagcc tggacattat gggcgcgctg
    10201 aatcctgtag tcctgccaag cgttctggcg ctggtgatga cggcagtatt tgatgccacc
    10261 ggaactatcc gtgccgtcgc cggccaggcg aacctgctgg ataaagatgg gcagatcatc
    10321 gacggtggga aagcactgac cactgactcc atgagcagcg ttttctctgg cctggtgggt
    10381 gctgctccgg cagcggtata catcgagtct gcggcgggta cggcggcggg cggtaaaacc
    10441 ggtttgacgg ctatcaccgt tggcgtgctg ttcctgctga ttctgttcct ctctccgctc
    10501 tcttacctcg ttccggggta tgcaacggct ccggcgctga tgtacgttgg cctgctgatg
    10561 ctgagcaacg tggcgaaaat cgactttgct gattttgttg atgcgatggc gggtctggtt
    10621 acggcggtat tcatcgtgct gacctgtaac atcgtaacag gcatcatgat cggcttcgcg
    10681 actctggtga ttggtcgtct ggtttccggc gaatggcgca agttgaacat cggtacggtc
    10741 gttatcgccg tggcgctggt gaccttctat gcgggtggct gggctatcta atctttcatc
    10801 ggattctgaa aacgggtggc aatggctgcc cgtttttatt ttctccgcac attgtgtgag
    10861 cttttgcgat attctgaaaa aatgagaatt caggcataac gtctttctca gatcataaaa
    10921 aaacatcgca aacagggaac gcatggaaat cttcttcacc atactgataa tgaccctcgt
    10981 ggtctcgctg tccggggtgg tcactcgtgt catgcccttt cagatcccgc ttccgcttat
    11041 gcaaatcgcc atcggtgcgc tactggcgtg gccgacgttt ggtttgcatg tggagtttga
    11101 tcctgaactc tttttagtct tgtttatccc gccgttgctg ttcgctgatg gctggaaaac
    11161 gccgacccgt gaatttctcg aacatggtcg agagattttt ggcctcgcgc tggcgctggt
    11221 ggtggtcacc gtggtcggca ttggcttcct tatttactgg gtggtgccgg gcattccgct
    11281 gatccccgcc tttgcgctgg cggcagtgct ttctccgacc gatgctgtgg cgctctccgg
    11341 gattgttggc gaagggcgca tcccgaaaaa aatcatgggt attttgcagg gcgaagcgtt
    11401 gatgaatgac gcctccggcc tggtgtcgtt gaagtttgcc gtggcagtgg cgatggggac
    11461 gatgatcttc accgttggcg gtgcgacggt cgaatttatg aaagtagcca ttggcggtat
    11521 tctcgccggc tttgtggtga gctggctgta tggtcgctcg ctgcgattcc tcagccgctg
    11581 gggcggtgat gaacccgcga cgcagatcgt tctgctgttc ttgctgccat tcgcttctta
    11641 tctgattgcc gaacatattg gcgtttccgg catcctcgct gcggttgccg ccgggatgac
    11701 catcacccgc tccggtgtga tgcgccgtgc gccgctggca atgcgcctgc gcgcaaacag
    11761 cacctgggcg atgctggaat ttgtctttaa cggcatggtg ttcctgctgt taggtctgca
    11821 gctgccgggt attctggaga cgtcgctgat ggcggcagaa atcgacccta acgtcgaaat
    11881 ctggatgctg tttaccgata ttattctgat atatgcggcg ctgatgctgg tccgtttcgg
    11941 ctggctgtgg acgatgaaaa agttcagcaa ccgcttcctg aagaagaagc cgatggagtt
    12001 tggttcgtgg accacacgag aaatcctgat cgcgtctttc gccggggtgc gtggggcgat
    12061 cactctggcc ggtgtgctct ctatcccgct gctcttgccg gatggtaacg tcttcccggc
    12121 gcgctatgag ctggtgttcc tggcggctgg cgtcattctc ttctcgctgt ttgtcggcgt
    12181 ggtgatgttg cctattctgc tacaacacat tgaagtcgcg gaccattcgc aacaattgaa
    12241 agaagaacgt attgcgcgag cggcaacggc agaagtggcg attgtggcga tccagaaaat
    12301 ggaggagcgt ctggcggcgg ataccgaaga gaatatcgat aaccagctgc ttacggaggt
    12361 cagttctcgc gtcattggta acctgcgtcg tcgcgccgat ggacgtaacg acgttgaaag
    12421 ttccgtgcag gaagagaacc ttgagcgtcg cttccgtctg gcggcattgc gttctgaacg
    12481 tgctgaactg taccacctgc gcgccacgcg ggagatcagc aacgaaacgc tgcaaaaatt
    12541 actgcacgat ctcgatttgc ttgaagcgtt gctaattgag gaaaatcagt aagccgaaag
    12601 cgacgtaacc aggcccgagg taatctgaaa aaggtgcaat cttttgcacc tttaacaggt
    12661 tagctcgggc taacattcat gattctaaaa caaataagtt atctctcttg taaagtcaat
    12721 tcattggctg ttcttcgact taacctcaat ctgcgtgttg cataatatct gcagtaatct
    12781 tcatagcgac tgcattaata ttatcctcag tttgtgacat gccagtatga gaggtacaat
    12841 cttcttcaaa gaatttctgt agtgttacca gggtatcact atcaatgggg atattttcct
    12901 ccccacggag aatagaaatg cccattccct ggacgctagc ctcatcaaaa ttaacccgat
    12961 agagacgggt attaaacata caggcttttg ttaaaataga acagttgaaa tggcagttgc
    13021 ttaaattggc attattaaaa ttaacattag ccatattaga gccggtacat ataaccattt
    13081 cgaggaatgt atttgttagc cacgtacaaa agagtttgct atcaattaag tttatattct
    13141 ggaggattgc tttgttcaga ttgacgcgag acagatcagc atgttcaaag ttaattaaat
    13201 cgagtttaga gaaactaagg tcggctttaa ataaattaac atatggcgct attactttat
    13261 aaagatttac ctcagccatg tacgccatat aaaaatttga atagtcaaga aaggtatttt
    13321 taatattggc tctattcatt gctgcattgt agaattgaac atgcatagcg gagcatgcat
    13381 ttagagttcc gtcggccatt tttatctgag cgaaatttga gtcatcgaga atacaattat
    13441 caaaaagtat tttttccatt atggcgtaat aaaaattcgt tttatacata ttacagtcct
    13501 ggaaaatagc gttttgtagt cagcaacctg caaaattcga attggataaa tcttcatctt
    13561 taatcacgat acctttcagg ctaaggtgtg aaaaatcgta accaccattt actgtctgtg
    13621 gaatttcgat agtattatta tggacggcga cattatttat aaaatatacc accttaatgg
    13681 atacttcctt ttctttacta aggtaaactc ttctcgtaat tctatcgt
//
LOCUS       DAZFVO020000130        13611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFVO020000130 DAZFVO020000000
VERSION     DAZFVO020000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.131"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            230..1060
                     /gene="yjiC"
                     /locus_tag="ACHKNB_RS18400"
                     /old_locus_tag="pgaptmp_003680"
     CDS             230..1060
                     /gene="yjiC"
                     /locus_tag="ACHKNB_RS18400"
                     /old_locus_tag="pgaptmp_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HGU4197122.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TTVLDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1201..1974)
                     /gene="uxuR"
                     /locus_tag="ACHKNB_RS18405"
                     /old_locus_tag="pgaptmp_003681"
     CDS             complement(1201..1974)
                     /gene="uxuR"
                     /locus_tag="ACHKNB_RS18405"
                     /old_locus_tag="pgaptmp_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HGU4197123.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2189..3649)
                     /gene="uxuB"
                     /locus_tag="ACHKNB_RS18410"
                     /old_locus_tag="pgaptmp_003682"
     CDS             complement(2189..3649)
                     /gene="uxuB"
                     /locus_tag="ACHKNB_RS18410"
                     /old_locus_tag="pgaptmp_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HGU4197124.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3730..4914)
                     /gene="uxuA"
                     /locus_tag="ACHKNB_RS18415"
                     /old_locus_tag="pgaptmp_003683"
     CDS             complement(3730..4914)
                     /gene="uxuA"
                     /locus_tag="ACHKNB_RS18415"
                     /old_locus_tag="pgaptmp_003683"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HGU4197125.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5254..6597
                     /gene="gntP"
                     /locus_tag="ACHKNB_RS18420"
                     /old_locus_tag="pgaptmp_003684"
     CDS             5254..6597
                     /gene="gntP"
                     /locus_tag="ACHKNB_RS18420"
                     /old_locus_tag="pgaptmp_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HGU4197126.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6840..7742)
                     /gene="fimH"
                     /locus_tag="ACHKNB_RS18425"
                     /old_locus_tag="pgaptmp_003685"
     CDS             complement(6840..7742)
                     /gene="fimH"
                     /locus_tag="ACHKNB_RS18425"
                     /old_locus_tag="pgaptmp_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4197127.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVVLYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7762..8205)
                     /locus_tag="ACHKNB_RS18430"
                     /old_locus_tag="pgaptmp_003686"
     CDS             complement(7762..8205)
                     /locus_tag="ACHKNB_RS18430"
                     /old_locus_tag="pgaptmp_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4197128.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8278..8808)
                     /gene="fimF"
                     /locus_tag="ACHKNB_RS18435"
                     /old_locus_tag="pgaptmp_003687"
     CDS             complement(8278..8808)
                     /gene="fimF"
                     /locus_tag="ACHKNB_RS18435"
                     /old_locus_tag="pgaptmp_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HGU4197129.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8818..11454)
                     /gene="fimD"
                     /locus_tag="ACHKNB_RS18440"
                     /old_locus_tag="pgaptmp_003688"
     CDS             complement(8818..11454)
                     /gene="fimD"
                     /locus_tag="ACHKNB_RS18440"
                     /old_locus_tag="pgaptmp_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HGU4197130.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSIIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNRSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFIVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11521..12246)
                     /gene="fimC"
                     /locus_tag="ACHKNB_RS18445"
                     /old_locus_tag="pgaptmp_003689"
     CDS             complement(11521..12246)
                     /gene="fimC"
                     /locus_tag="ACHKNB_RS18445"
                     /old_locus_tag="pgaptmp_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4197131.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12283..12822)
                     /gene="fimI"
                     /locus_tag="ACHKNB_RS18450"
                     /old_locus_tag="pgaptmp_003690"
     CDS             complement(12283..12822)
                     /gene="fimI"
                     /locus_tag="ACHKNB_RS18450"
                     /old_locus_tag="pgaptmp_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4197132.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSEHVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12887..13435)
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS18455"
                     /old_locus_tag="pgaptmp_003691"
     CDS             complement(12887..13435)
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS18455"
                     /old_locus_tag="pgaptmp_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4197133.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3328 a         3492 c         3270 g         3521 t
ORIGIN      
        1 caacatcaaa ttccaattag taataagata tgctttttaa ttgcatgaaa ataattttaa
       61 atgagcgttt tttgatactt tagagtgttt cctacccttt aatatcacca tgtagagttg
      121 atatcactac aacagaaaca tgcatgtcat ttgtttactt cttttttact acagaaaaat
      181 ttatctattt ttctaaacat cagtgaaata ctcataaaag gtaactatta tgtctatgcc
      241 attaagcaat gcccttcaga gtcaaattat tacagataat cacttccttc atcatccaaa
      301 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      361 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      421 tagaaaactt gatattagta aagagccctg gccatttaat tatgtgacag aaacatgcag
      481 agaatccgat ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcaccac
      541 agttttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcca cagagttagc
      601 agaaggatta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      661 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      721 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      781 gattgatatc tatattgatc agataatgac tgtctcaggg gtggcaaatt cttccggatt
      841 cgcccttgcc gcattactta atgctaatat tgagttgggt aatgacccaa taattggtat
      901 agaagcctat cccggtaccg cggagatcca tgcaaaaatg ggatataaag ttatcccggg
      961 tgatgaaaat gcaccactca aacgtatgac tctgcaaccg tcatctcttc cagaactgtt
     1021 tgaattaaaa aatggtgaat ggaattatat tggtaaataa ctaccaggaa caatgagtac
     1081 taatattcag gttatacaga tatttaaaca ataccctgca atttacaaag taatactcat
     1141 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1201 tcaggcgtcg actttatcca gcggccatga atcaaacaga tagccatcaa aataaatatc
     1261 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1321 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1381 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1441 attgttttcc cgccactgcc aggactgacg gaacagctcc accagcatgc tgttatgcgt
     1501 tgcttcggca atagcgagat ggaactgcat gtcaccgctt tcgctgctgc ctggcgcact
     1561 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1621 ggtagcctgc aaagcggcaa actcggcgat gttgctctcc aataactgcc gcgcctgtaa
     1681 cagctcaaaa ggaccggcat cgttgcagac gttggcatcc ggactgtctg tgttctggct
     1741 gcctgagtta tcaagaacat agataccggc accccggcgt acttccacca gccctttgat
     1801 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     1861 ttcacgctcc ggcggcagcc gttcgccagg gttgtacggc gtctttatga tcagatcgcg
     1921 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtggcagatt tcatggtgtt
     1981 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2041 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2101 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2161 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2221 gttcgcacag ctgctgatat gccgccgtca ctgcgccaac aaagtcggca ttctgcggca
     2281 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2341 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2401 cctgctcatc cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac
     2461 gccagctacc gccgttttgc aggtgcagac gcaccggatc cagcagacgc tgcggtaact
     2521 tctggctgcc gtccattgca atctgccagg tacggtgacg cagagatggg ttgctgaaac
     2581 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2641 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2701 tgtcggcaat agtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2761 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc
     2821 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     2881 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     2941 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcatggg aaggtgacgt
     3001 tctcttcaat ccatgccgcc agctgcggat cgcgtgcctg agccagtccc agtaccgcga
     3061 ccttcgccac atgaccgttt tcacgcacgt tatcgcagga catcaccgta aacgctttca
     3121 accctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3181 cagtcgggtt ttccagatcg tgcttgatca gtgggttatt gagatccagt tgaccgcttg
     3241 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3301 gcgccatcgc gttgagaata ccttcgcagc catcgatttc cggatgcagc gcttctttca
     3361 tcgaaccgat aattttcagc tcggtgctct ctgcgccttt ttccgctacg gtgtacagca
     3421 gttgctgttt tttcaggttt tcgatcagca cgcggtcgtt gcctggcatc aggttaactt
     3481 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3541 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3601 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3661 gaatccgtct tcaaagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     3721 cggtggatat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3781 ggccaggcct ttcagacgac caattgcgga gtaacctggg ttggttttct tcttcaggtc
     3841 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     3901 tttacgacgg tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc
     3961 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4021 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4081 acgcacgccg taggaaccgg tgcacatggt aaaaccgttt gccatgctgt ttacggtatc
     4141 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaggccga ggatcgggcg
     4201 cggcggatcg tccgggtgaa cagccatacg cacgccaact tcttcggcaa ccggaataat
     4261 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4321 ctccaggtgc ttacggaact ggtcgagggt gtacccttct tccgcgcccg gcagaccagc
     4381 gatgatgtta cgggtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4441 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4501 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4561 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4621 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4681 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4741 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4801 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     4861 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     4921 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccctggag ttgtttaggt
     4981 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5041 tctacctttt ggtaagacat gtcaacccga attctgaaat tggttaacca catcacaaga
     5101 attttactct cctggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5161 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5221 taacaacggt tcaaccaggt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5281 gtattcggca ttggtctgat gctggtactg aatttgaagt tcaaaatcaa ttcaatggtg
     5341 gctttgttgg tggcggcgct gtccgtcggg atgctggcgg gcatggattt gatgtcgctg
     5401 ctgcacacca tgaaagcggg cttcggcaac acgctggggg aactggctat catcgtggtg
     5461 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcgcat
     5521 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcttg
     5581 atttttggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5641 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5701 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5761 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     5821 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     5881 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     5941 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6001 gtaaaagata cccctgcctg ggaagtggtt aactttatcg gttcctcgcc gattgcaatg
     6061 tttattgcga tggtggttgc attcgtactc tttggcaccg cacgtggtca tgacatgcag
     6121 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6181 ggtgcgggtg gcgtgctgaa gcagactatc atcgacaccg gcattggcga caccatcggc
     6241 atgttgatgt cccacggcaa tatctcgccc tacatcatgg catggctgat cactgtgcta
     6301 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattataagt
     6361 gctgcaatcc ttgatccagc aactggtcag ctggttggcg tgaatccggc gctgctggta
     6421 ctggcgacgg ctgcgggttc caacaccctc acccacatta acgatgcatc tttctggttg
     6481 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6541 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6601 gacaccagag cctgccaatg gcaggctctg actgatgaca aagtctaaaa atgtgcccag
     6661 acagtcctct cgcccctctg gggagagggt tagggtgagg ggaaaagacc agcaaagtgc
     6721 gctttgttta tgccagatgc gacgctaacg cgtcttatct ggcctacaaa gggctaacgt
     6781 gcaggttttt agcttcaggt aatattgcgt acctgcatta gcaatgccct gtgatttctt
     6841 tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct
     6901 ccggtacgtg cgtaatttgc cgttaatccc agacttaccg ccgaagtccc tactgctcct
     6961 aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg
     7021 ccctgtgcag gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc atctgcggtt
     7081 gtgccggaga ggtaataccc caggttttgg cttttcgcac aataaacggt aagaggaatt
     7141 ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga aacatcgcag
     7201 ccgccagtag gcaccaccac atcattattg gcgtaaatat tccacacaaa ctggaaatca
     7261 tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga gccagcttta
     7321 atcgccaccc cgcccgcact gctcacaggc gtcaaataaa gcaccaccgg ccacggctta
     7381 tccgttctcg aattataaac aacgcgcggc gtttcgctgg tggtaggaaa tggatagcta
     7441 ctgccactat attttacggt cccggaaaaa ttagataaca cgccgccata agccgagcct
     7501 cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc
     7561 gtcgaaagat ccacgaccag gttttgcccc acattcacgg cgggcgcaag gtttacataa
     7621 acattggcgc tgccaccgcc aatagggata gcggtaccat tggcggtttt acaggcgaat
     7681 gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat aactcgtttc
     7741 attacaatca tctcttcggg ttcagctgta ggtataggtg atgctaatca ctgcctgaat
     7801 ggttccctga gtggctccgc catttaccgt caatgctctg acctgtaacg ggaagtgcgc
     7861 tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccagtattca atgtgttgcc
     7921 actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc
     7981 ggtactgtcg gctgccccgc tgaagctggc agtgaccctc gacgttccca ccggacaatt
     8041 agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca tcagactgaa
     8101 agaataaaga tcgccgagat caaccgtggc attggtggtg gaaactgtgc acggtttggc
     8161 gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag
     8221 cgccaatatt gccgccaata catacccacg tttgcaccat ttcatgagca tctccagtta
     8281 ctgatattca agagtgaagg tagccgtggc attgatatgc cctgcagtga caggcacctg
     8341 tgtcgccatt agccgggcgt aaaaattcag cgtatttggt ttacccggcg tcagggtcgt
     8401 ccacgaaagc gcggacgatg gagcattaag gggtatttga ttttgctgct cattcagaag
     8461 ctgtattccc agtcccgaag ccgctgacac cgtattttca agtgcaagca gattggcatt
     8521 gtggctatct gcaacgccgg taaacccaac ttttacggca gaaacggcat taccacaggg
     8581 tgacagcaaa atacgaaatg gaacaacagg agtcgtcgcg ccaatgttgt taaattgctt
     8641 cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc
     8701 gttatccctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag cgtgactcac
     8761 cgccagccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca cgccccctta
     8821 acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata
     8881 attggcgaca cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccgctaa
     8941 aggcattccg ctgaggtaaa cctgaccatt atccgcaaca atgccgctac tctggctact
     9001 ctctgatgtc accatcgccc caaacggcag cggcttatta ttgtgggtca gcgtcatgag
     9061 cagttttatc ccaacgcgcg ctttaaactc tgctcgcacg atcgccccac gagtgggaac
     9121 aacgttagca accgcgttat ctaaatcgac gttatcagcc agggtattgg tatccagcgc
     9181 cactctattt tcccgatatt cagtggcata aggcagcacg gcataaccac gccagtcggt
     9241 acgcaccccc gtctggtttt cgacttttgc atcttttgcg ccaggcgctt taacaagcac
     9301 cactgtttcg tttaacggct gccccagcgt tacgccattg gcatgagcca ataccccacc
     9361 gctgactccg taatagagct gcttaatatc atcgctatgg ctgtaaccga tattggcatt
     9421 gccgtaacca ccgcgataat tcagcgtggc gtagcctgta cttccgctat ttccatcgcc
     9481 tcccccggca tagccggttt gcacgctata gctgaggttg ttgtcttcca gcaacgtacc
     9541 gtatacacca gccagattgg tcatccgacc gttgagatcg tgtgacatgc tgtagctggc
     9601 actggcatgt cgccactgag atttactgtc agaacgcagc cagtggctga aaggaatatt
     9661 gacgttaagc gctaacatct gatcccgtcc tttttgccag gcgtttttcg tcaggctata
     9721 gctgagcgtc cagttgatat cttcgaatgc agtatttaaa ccagcctgga attgctcatc
     9781 gacattactc gttccccaat aagtttgatg gctaccactc aaatacagtg ttgatgtgcg
     9841 cccgagttgc tgagtaacgg tgagctgtaa tttcccgcgt ttgttataag cgaggttgta
     9901 atagtcggtg aatttcggct taacctgaat aactccgtcc tgtgtttcga tgttgtagcc
     9961 attcattcga ctgtatgttg tatcagcgaa attaaaatat ccgctggtcg aataacggta
    10021 acccactaac tgaatattcg tgcctgactc attgagcgat ttgttataga gaaaacgcac
    10081 cgattgtccg tcatgctgac tgtcatcggg aagtgtggaa ttagcctgcg tcatatccac
    10141 agacagagcg cccagtgccc ccatgttttt cccgataccg aaattaaaag cacgataacg
    10201 atccgccagt tgcgttccac catatattgt ccagccagcc ggaaggccgt ggagtaatgt
    10261 actctggaaa aagcggggtt tttcctgctg cgcatttcca ctacggtatt ctcctgccgt
    10321 aatggaataa cgagtatgcc cttcacgttg caaaagcggg actgacgaat agggtacgat
    10381 aaaaatctgc gtgctgccgt cagcctcttt gatcgttacc tgcaagtcac cactattacc
    10441 tgcggcatag atatcgttga tggtaaaagg ccccggtggc accgtactat tataaatgtc
    10501 atacccattt tgtttaatag tgacctgtgc agtaccacga gcaataccgt ggatcaccgg
    10561 ggcaaatcct ctttggctat cgggtaacat attgtcatct gaggccaatt gtgcgccgcg
    10621 aaagttaata ccatcgaaaa tatcgccctg agtataacca tcacccagcg tcagccggga
    10681 acgtaacggt attatgtctc gctcaagcca ggtattgata tgctgccatt tatttttgct
    10741 acctgatgat ctgtcgctac ggttataact ccaggtggta ttgtcgcgta aacgccacgc
    10801 accaatattt aacccactct gtaggtttaa atatgcataa tggctgttac ccccaatccg
    10861 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
    10921 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
    10981 caggttgagt cgctgctgac caacatctaa atgcgcagta gcgtcatgga ttattgaggt
    11041 taatggcacg caggcatcat ccgccagcag attcataccg gagacagaag ccgtattcag
    11101 ccccatactg gcaagttgcg cgcgtgtcag gcagggaaca atcccttgtt cgctgtcgcc
    11161 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacacg
    11221 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcag ccacagcctg
    11281 gggatcatcc gctaaaaagc gcggattaaa atagagttcg gcagatgaca aaggtgtctg
    11341 tgcggcaaaa gcacaggcga caaagagccg gacaaaaaaa ccagccaaac gatgcttacg
    11401 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
    11461 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
    11521 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
    11581 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
    11641 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
    11701 tgtcgggtta atcagcgtca gggaattcgc gctacgacga aatcttaatt tttctgcggc
    11761 ctgatcgggt ggcaacgcta atttagccgg acgatagtac agtttaatgc ggctgataat
    11821 tgcgagctgt agcgtattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
    11881 attcatccag aataaacttt cccgatcctg tggcaattgg ttattagttg catcaagaat
    11941 acgcaaggta ttctctcttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
    12001 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
    12061 atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
    12121 cgcacctaag gccactcccg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
    12181 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
    12241 actcactgtc ctgttcctgt tatcacatta tctgctgaac aattactgat aggttaaaga
    12301 gaaccaggcc tgtgcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
    12361 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
    12421 attaatcggt acgaggtttc cttcatcatc aaacaacgct acgccaatat tggtggctat
    12481 ccctggcccc tctccaacgg aaagcacatc tggattttta ccatccgcga caccgtgaaa
    12541 cgccacaccc acatgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
    12601 caccggtgcg ctatcttccc caaccgcatg aaaccggtta ctgctgattt gccccatatt
    12661 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
    12721 aaattgcata tttccgccgg gcaacgtggt attccattta tttccggcca gagcaaacat
    12781 tggcagcaac gacgctaata gaaataatct cttccttttc atcgttattt ttatcgcaca
    12841 agggtgggca tccctgcccg taatgacgtc cctgaacctg ggtaggttat tgatactgaa
    12901 ccttgaaggt cgcatccgca ttagctgcgc caggggttgc ggcaccggtt gcaaaataac
    12961 gcgcctggaa cggaatggtg ttggttccgt tattcagggt tgtttctgaa ctaaatgtcg
    13021 caccgtccag cgccagcgca gcacccgttc tgtccaggat ctgcacacca acgtttgttg
    13081 cgctacccgc agctgaactc tgcagagcca gtacatcagt atgcgtagca tcaattgccg
    13141 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
    13201 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
    13261 ggcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
    13321 cttccccttt aaagtgaacg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
    13381 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
    13441 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
    13501 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
    13561 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat t
//
LOCUS       DAZFVO020000131        13301 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFVO020000131 DAZFVO020000000
VERSION     DAZFVO020000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.132"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..543)
                     /locus_tag="ACHKNB_RS18460"
                     /old_locus_tag="pgaptmp_003692"
     CDS             complement(<1..543)
                     /locus_tag="ACHKNB_RS18460"
                     /old_locus_tag="pgaptmp_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4197134.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSAGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGI"
     gene            complement(1569..2564)
                     /locus_tag="ACHKNB_RS18465"
                     /old_locus_tag="pgaptmp_003693"
     CDS             complement(1569..2564)
                     /locus_tag="ACHKNB_RS18465"
                     /old_locus_tag="pgaptmp_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HGU4197135.1"
                     /translation="MGLFCLRATPGAYQPQSLSQMVFQESNNRDVARKYAEYITSVPL
                     RRFVAEKVREYCGDLPLSIFDGACGSGQLEQFLTVGSLYAVDIQASPLLHIPDNFPDA
                     TVKTVNENFFNAIDDVSDTDTFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGV
                     LDDAFYVLASMKARFSFFLCFPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSV
                     LFVIIDNEKQSDDVVVYGVGFNGETCKKSAPVTEKINTDNWNRPYIAEEKEVIDIVQV
                     TAELLVRQTRRRKLEDEHNAFVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            complement(2590..2666)
                     /locus_tag="ACHKNB_RS18470"
                     /old_locus_tag="pgaptmp_003694"
     tRNA            complement(2590..2666)
                     /locus_tag="ACHKNB_RS18470"
                     /old_locus_tag="pgaptmp_003694"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Arg,seq:tct)
     gene            complement(2680..2756)
                     /locus_tag="ACHKNB_RS18475"
                     /old_locus_tag="pgaptmp_003695"
     tRNA            complement(2680..2756)
                     /locus_tag="ACHKNB_RS18475"
                     /old_locus_tag="pgaptmp_003695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2720..2722),aa:Arg,seq:tcg)
     gene            complement(2764..2839)
                     /locus_tag="ACHKNB_RS18480"
                     /old_locus_tag="pgaptmp_003696"
     tRNA            complement(2764..2839)
                     /locus_tag="ACHKNB_RS18480"
                     /old_locus_tag="pgaptmp_003696"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2804..2806),aa:Ile,seq:cat)
     gene            complement(2880..3928)
                     /locus_tag="ACHKNB_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /pseudo
     CDS             complement(2880..3928)
                     /locus_tag="ACHKNB_RS18485"
                     /old_locus_tag="pgaptmp_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(4079..4276)
                     /locus_tag="ACHKNB_RS18490"
                     /old_locus_tag="pgaptmp_003698"
     CDS             complement(4079..4276)
                     /locus_tag="ACHKNB_RS18490"
                     /old_locus_tag="pgaptmp_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4197136.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(4296..4493)
                     /locus_tag="ACHKNB_RS18495"
                     /old_locus_tag="pgaptmp_003699"
     CDS             complement(4296..4493)
                     /locus_tag="ACHKNB_RS18495"
                     /old_locus_tag="pgaptmp_003699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197137.1"
                     /translation="MMTSLLPLCKRHLNGAFVVFIKRSPPLAGFFMPEKRHRALNALV
                     VANTGLFSLLAFLTRVIGVSR"
     gene            complement(4619..5020)
                     /locus_tag="ACHKNB_RS18500"
                     /old_locus_tag="pgaptmp_003700"
     CDS             complement(4619..5020)
                     /locus_tag="ACHKNB_RS18500"
                     /old_locus_tag="pgaptmp_003700"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HGU4197138.1"
                     /translation="MVLKYQPSVRSVLMCDFRGMVVPEIVKVRPVVVVSRNRHNNQLV
                     TVVPISTTEPIPRRDCHHELSENPIPGNEHITCWVKCDMLMTVSLSRLDRIKTRTWEG
                     RNYIVPMIAEDEFENIKRAVLHGIGMAYLYR"
     gene            complement(5214..5903)
                     /locus_tag="ACHKNB_RS18505"
                     /old_locus_tag="pgaptmp_003701"
     CDS             complement(5214..5903)
                     /locus_tag="ACHKNB_RS18505"
                     /old_locus_tag="pgaptmp_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HGU4197139.1"
                     /translation="MNNQYLQFVREQLMIATADLSVSIKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIALVSPVEFSTSSWRRAVLELEAHQK
                     AWLLWCYGGNICWEYQIAITQWAWNEFNTQSGTRKIAGKTLERLKKLIWLAAQAVKAE
                     LFGGEGYEYRDLASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     TFSQQNIAKLD"
     gene            complement(5896..6258)
                     /locus_tag="ACHKNB_RS18510"
                     /old_locus_tag="pgaptmp_003702"
     CDS             complement(5896..6258)
                     /locus_tag="ACHKNB_RS18510"
                     /old_locus_tag="pgaptmp_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4197140.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     QLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            complement(6271..7320)
                     /locus_tag="ACHKNB_RS18515"
                     /old_locus_tag="pgaptmp_003703"
     CDS             complement(6271..7320)
                     /locus_tag="ACHKNB_RS18515"
                     /old_locus_tag="pgaptmp_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4197141.1"
                     /translation="MRVLLRPVLVPELGLVVIKPGRELLPVFHRGRVLVEPEPKSMRS
                     LPSGVVPVVCQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            complement(7322..7594)
                     /locus_tag="ACHKNB_RS18520"
                     /old_locus_tag="pgaptmp_003704"
     CDS             complement(7322..7594)
                     /locus_tag="ACHKNB_RS18520"
                     /old_locus_tag="pgaptmp_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197142.1"
                     /translation="MTQHIKSHNSEADPEIKRGWRFRTPQYGWFHYLFCTTDEADMLQ
                     EAYLRRGVRVERSLNVDRLTWTVSVYLPVRAHLPRTNACYRQRVWR"
     gene            complement(7717..8061)
                     /locus_tag="ACHKNB_RS18525"
                     /old_locus_tag="pgaptmp_003705"
     CDS             complement(7717..8061)
                     /locus_tag="ACHKNB_RS18525"
                     /old_locus_tag="pgaptmp_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HGU4197143.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIIKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(8183..8338)
                     /gene="hokD"
                     /locus_tag="ACHKNB_RS18530"
                     /old_locus_tag="pgaptmp_003706"
     CDS             complement(8183..8338)
                     /gene="hokD"
                     /locus_tag="ACHKNB_RS18530"
                     /old_locus_tag="pgaptmp_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4197144.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(8629..8982)
                     /locus_tag="ACHKNB_RS18535"
                     /old_locus_tag="pgaptmp_003707"
     CDS             complement(8629..8982)
                     /locus_tag="ACHKNB_RS18535"
                     /old_locus_tag="pgaptmp_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HGU4197145.1"
                     /translation="MIDRTKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMCKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(8997..9347)
                     /locus_tag="ACHKNB_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /pseudo
     CDS             complement(8997..9347)
                     /locus_tag="ACHKNB_RS18540"
                     /old_locus_tag="pgaptmp_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9349..9693)
                     /locus_tag="ACHKNB_RS18545"
                     /old_locus_tag="pgaptmp_003709"
     CDS             complement(9349..9693)
                     /locus_tag="ACHKNB_RS18545"
                     /old_locus_tag="pgaptmp_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HGU4197146.1"
                     /translation="MSGINYQALREAAQNYQSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRIDEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(9680..9985)
                     /locus_tag="ACHKNB_RS18550"
                     /old_locus_tag="pgaptmp_003710"
     CDS             complement(9680..9985)
                     /locus_tag="ACHKNB_RS18550"
                     /old_locus_tag="pgaptmp_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HGU4197147.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHKAEEALKREGHTVLNPSVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEWD"
     gene            complement(9982..10263)
                     /locus_tag="ACHKNB_RS18555"
                     /old_locus_tag="pgaptmp_003711"
     CDS             complement(9982..10263)
                     /locus_tag="ACHKNB_RS18555"
                     /old_locus_tag="pgaptmp_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4197148.1"
                     /translation="MILRQCAGTIKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     QSAKYSQRDIEQARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVQA"
     gene            complement(10296..11012)
                     /locus_tag="ACHKNB_RS18560"
                     /old_locus_tag="pgaptmp_003712"
     CDS             complement(10296..11012)
                     /locus_tag="ACHKNB_RS18560"
                     /old_locus_tag="pgaptmp_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HGU4197149.1"
                     /translation="METVFDALKALKKASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVKETT
                     TQEEIKTESVAVTVQSQPSFTRKHQDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            complement(11046..11507)
                     /locus_tag="ACHKNB_RS18565"
                     /old_locus_tag="pgaptmp_003713"
     CDS             complement(11046..11507)
                     /locus_tag="ACHKNB_RS18565"
                     /old_locus_tag="pgaptmp_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HGU4197150.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGTNPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWTKHVGNGMSVPPVRRQLEGAKHPKGPTPIEWLKQEYERRKAAGFI"
     gene            complement(11500..12510)
                     /locus_tag="ACHKNB_RS18570"
                     /old_locus_tag="pgaptmp_003714"
     CDS             complement(11500..12510)
                     /locus_tag="ACHKNB_RS18570"
                     /old_locus_tag="pgaptmp_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HGU4197151.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTELKELNPTHNVHE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            complement(12597..13034)
                     /locus_tag="ACHKNB_RS18575"
                     /old_locus_tag="pgaptmp_003715"
     CDS             complement(12597..13034)
                     /locus_tag="ACHKNB_RS18575"
                     /old_locus_tag="pgaptmp_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4197152.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDSGSSGESVVVH"
     gene            complement(13018..13299)
                     /locus_tag="ACHKNB_RS18580"
                     /old_locus_tag="pgaptmp_003716"
     CDS             complement(13018..13299)
                     /locus_tag="ACHKNB_RS18580"
                     /old_locus_tag="pgaptmp_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4197153.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTQTLTQPLSRSLIHENQA"
BASE COUNT         3183 a         3766 c         2933 g         3419 t
ORIGIN      
        1 aatacccgca ttcgccggta taaacggcag cagttttttg gcgatatgca gcccctgccc
       61 cacggttccg tactgcccct ttgacaggtc cgctttcgga tggttaagac ggctcatgtc
      121 ctgcacatca tgcagacaat ggtccgccgg aataatgtcg ttatacttac aggcggcacc
      181 acccggtgtc actgtactgc ggcgcgccag ctgcttaata cgcgggtccg gacggtcata
      241 tgtctccggc aacggaaggc cttcaccata tgccatgctg tttgactgcc ccgccagaac
      301 cacaacaaag taatactccg ggtctctggt ggcgctgatt actgtgcctt ctccagccga
      361 cggcttcacc aacacaggtg tggtgacatc accttccgcc gcaatggcct gcatcagggt
      421 ataaggcgtg atggcgacag gactgccaaa tggctgccac ccctccttca gtttttgagt
      481 cagtcgctcc gcaaggtctg acggcgacgc cgccctgacc acgtcatagt gtttaaatgc
      541 catgaatcct cccggtcggg ataatgttgt gagtcagatg aggggcgggc tgaagtccgg
      601 aagttacagg acaatggcag gaggaagact acagcccgca atacgaaaaa ggccgcgcag
      661 ttgcgcagag tgattactgt cgggtattat tctccagctg aaaaattgat tacttcacgt
      721 tttgttgttt attccttgct gcccgcatct caccatgcgg gcttttttta tccataagaa
      781 agcccctccg gagaggggct gaagagtagc gctatgtacc gttcattaca tgatgccggg
      841 tgcctcccgg tgagttcagc ccggtgccac cgaacccgcg tcattctcgc taacgatcat
      901 cagagaccat tccgtacacc agccgcccct ccgcacaggg ggattcatca tgcagaaatt
      961 ttttaacaaa ttctcagtcg ggaagacaac cttcaactga ctgaattgtg agcaacatag
     1021 catttaacgg ggaacctgtt ttctgcagta aaaaggccca ccggagcgga cgggcctgga
     1081 aggatagcgg tcatgtgatg ccggtttccc ggtaactcag caccggtatc tgagtcaacg
     1141 ttttctctac tgggtcattt ccgatacgcc ctgcctgctg acaggctttc atcacatctg
     1201 aaaatatagc accctgactg atactgtagt acccaaggtt ccagaaactg tgatgtatcc
     1261 ggcacagaaa agcccctccg gagaggggct gacgctgcgt atctgtatca tcatgcgcat
     1321 ggtgccgggt gcctcccggt gagttcagta tcagcacctg aacccgcaca gaaaggataa
     1381 gggatgtaac caaacacctg tcgctgatat gcccctccgc tcagggggat tcaccatgcc
     1441 agtttctttt aacaaactcc ccgcaaatca gacaactgtc aaccgtctga attgtgagac
     1501 atttaaaatt ttcggggcaa caggaagaca atcatcacga caccggcaac cagcgaccgg
     1561 caccagattt aacgtatgtg cacacaacct gaaccggcat caaaaaacaa accgtgcact
     1621 gacagaaaac gggaaacctc cgtccgctga tcatcactca ttaaggacca cacaaaggcg
     1681 ttatgctcat cttcaagttt ccgtcttctt gtttgcctta caagcaactc agccgtcacc
     1741 tgtacaatgt caatgacctc cttctcttca gcaatgtacg gcctgttcca gttgtctgtg
     1801 ttaatcttct ccgtcaccgg cgctgacttt ttgcaagttt ccccattaaa cccgacgcca
     1861 taaacaacaa catcatcgct ttgcttttcg ttatcaataa tgacgaataa tacagaaacc
     1921 gatgtatcag cgaacccgcc tgaaatctga accaactcaa caacgcggtt gccaaaatac
     1981 tgacgcattg ccagctcacc acgcctgtaa ccaaccccgg gaaagcacaa aaaaaatgaa
     2041 aatcttgcct tcatgctcgc cagaacataa aacgcgtcat ccagaacacc ggaggtcttt
     2101 ttataaggca tcatcgctga aatcttctcc ctgatatccg cgctctgtgc tttgtactgt
     2161 agcgaaaaag gcggattcat gatgacacaa tcgaacgtgt ccgtgtcact gacatcatcg
     2221 atggcgttga agaaattttc attgaccgtc ttcactgtgg catccgggaa attatccggg
     2281 atatgcaaca acggagaggc ctgtatatcc acagcataca gacttcccac cgtgagaaac
     2341 tgctccagtt gcccggagcc acacgcacca tcaaaaatac tcaggggcag atcaccacaa
     2401 tattcccgca ctttttcagc gacaaaccga cgcaaaggaa cggacgtgat gtactctgcg
     2461 tattttctgg cgacatcgcg attattggac tcctgaaaaa ccatttgtga aaggctctgt
     2521 ggttgatatg cgccgggcgt ggcgcggagg caaaaaaggc ccacaaaagc gagccaggaa
     2581 aaataagtgt ggtgcgttgt actggattcg aaccagtgac cgattgctta gaaggcaatt
     2641 gctctgtccg gctgagctaa caacgcagtg tacagataat ggaccgccat cgaggactcg
     2701 aaccccgcgc aaccagcttc gaaggctggc gctctatccc gatgagctaa tggcggtatg
     2761 tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     2821 accactgagc taaagggccg ggcgcaggat aataacggta cgtaactaat cctgcaatat
     2881 catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa acgttcagtc
     2941 tccagctcaa cgccagttgc acgacgcccc agcgccatcg ctgctttgac tgtcgaaccc
     3001 gaccccatga aaaaatctgc aaccaggtca cccggacgac tgctcgcgct gattatctgc
     3061 tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg caccggttta
     3121 tgcgtccaca catcggtgta cggcacctgc gccgtcacac cgaaataccg ccgcagatgc
     3181 ttatattcac tctgcagttc cgtatagtgt cggttcagtt cactgtatgt gctgaccagt
     3241 tggtgatgtg gcttttccag ttctccgcgc tgatgcttct cttctgccac ccgggcaaac
     3301 agcgcctgta atttcagata atcgctttcg tccggtagct ggcactgaac cagtgcgaca
     3361 ccatgttttt ctttcctgtg gcatcagcaa tctgttttgc cgttatcccc agggcagcac
     3421 gtgcatcacg aaagtaagca atcagcgggg ccatcacatg ctgtttcagt gccctgccct
     3481 tcgcctcata cccggcatct ttcggacgat acggcccctg ataatgttcc gcgaacagaa
     3541 tgcgctctgt ggcggggaaa tacgccctca ggctttcctt gttgcacccg ttccagcgtc
     3601 cggacggctt cgcccagata atatggttca gcacactgaa gcgttcacgc atcatgattt
     3661 caatgtcaga tgccaggcga tggccacaga acaggtaaag acttccggca ggtttcagca
     3721 cccgccagaa ctgcgccaga cactggtcca gccacttcag gtaatcatcg tcgcccttcc
     3781 actggttatc ccagccctca ggcttcactt taaagtacgg cgggtccgtg actatcaggt
     3841 caacagaatt ttcgggtaac gaccggataa attccaggca gtcggcgttg attaactcac
     3901 aactgtatat ttttacagca ttaagcatgg atcattaagc ctgtctctga taggctcatt
     3961 ctgcttttgc gcaaagcagt gggcctgagg tttgcttgtg atccggacgc atgagcagat
     4021 ggctggtgag tgcccctaac acccaccagc cgcccattta ccacaaataa aaaagccttc
     4081 aggactgaag gcgtctgtaa caaccgaact gatagtctgc cagacccgcc ataacaagct
     4141 gggtcagtat taactggcag cgttcacgtg aaagataagt attctgcgca atctccccga
     4201 ctgtcgccgg ttcggtggca cttaattcat taaacaccac tctggcggtt tctgtcatat
     4261 cctgctgttt cagcatgtct ttttcctttt tccggttaac gtgacacacc aataactctt
     4321 gtcaaaaaag ccagcaagct gaaaagacca gtattcgcaa ccaccagcgc gtttaacgcc
     4381 ctgtgtcgtt tttcgggcat aaaaaaaccc gccagtggcg gggatctttt gataaaaacg
     4441 acaaaggcac cattaaggtg ccttttacac aggggcagca ggcttgtcat caccttattg
     4501 ctactcctgg cttccagttc ggaagtctca caagtcccat cagggtacag cgacagtatc
     4561 agtcaacaca ctatgtgtgt caatataaat tcatatcgaa ttcgataatc gttttcgatt
     4621 atcgatacag ataagccatc cctatcccgt gcaataccgc ccgtttaata ttctcaaact
     4681 catcttccgc aatcatagga acaatataat ttcgcccctc ccaggttcta gtttttatgc
     4741 gatccagtcg gctcaatgaa accgtcatca acatgtcaca ttttacccaa caagtaatat
     4801 gctcattacc agggatgggg ttttctgata actcatgatg gcaatctctg cgaggaatcg
     4861 gttcagtagt gcttattggt accactgtta ccaattgatt gttgtgtctg tttctggaca
     4921 cgactaccac tggcctgacc ttgactattt ccggaacaac cattccccga aaatcacaca
     4981 ttaaaacaga acgaacagac ggctgatact ttaacaccat aaatctcttt gcctgataat
     5041 gacgacttgt gaactccact acaaacaaaa tgattctgct gaacaggcag tttcgcactg
     5101 tacattataa cctgttaagc caacaaaaaa acccgctcag cggcggggtt tgcttacttt
     5161 gccatcgcgt acaaaatcgg caaaatatca gatttacatg aaatatatgc tttttaatcc
     5221 agttttgcaa tattttgctg tgaaaatgtg gtcttttgtt ttgaacgtgt tttcgttaaa
     5281 agcaataaag cttggctatc aagctgtaga aaaatgtgct tcattgcaac ccagcgttca
     5341 gtaaatgtct cagaccagtt ttttgatgtc actcccacca gtgatgccag atcccggtat
     5401 tcataacctt ccccaccaaa aagctcagct tttaccgctt gcgccgccag ccagatcaac
     5461 ttcttcagac gttccagcgt tttcccagct atttttctgg taccggattg agtattaaat
     5521 tcattccacg cccactgcgt tatcgcaatc tgatactccc agcaaatgtt tccgccgtaa
     5581 caccacaaca accacgcctt ctgatgcgct tccagttcca gaactgcacg tcgccatgat
     5641 gatgtcgaaa actcaaccgg actgaccaga gcaattgacg tccctttcgc cagtgattgt
     5701 ttacccggga tcggtgggtt atcccgcgtg atcatttttc cggttacctc atcgcggtaa
     5761 cgaatttttt tgcgtctgta acgccctgta tcgaacagtg cattttcctg ccaggcttcc
     5821 agttggcctt ttatcgacac actgagatcc gcagtggcaa tcatgagttg ctcacgaaca
     5881 aactgtaaat actggttatt catgcacacc cacctctgta attcttatct ccagccgtcc
     5941 accagatact ggctggccac gtacaatatt gatttcatca aactgctcat cgtccattaa
     6001 caaccctgca tgcgtcagcg catccagcgg cgctttcagg atattatcca ggtcacggcg
     6061 gcgcttatcc ggtggctctg caatcacctt tatcgccagc cttccggaca ggcttaattt
     6121 cagctgctgc tggcgaacaa taagcgccac tgcccggcga tagcgctttc cctcctcaga
     6181 tataaaatat gtgctgccac gacgtcgcca gtaagtattc accgtcggcg gataaggcaa
     6241 aacaaactct atacgcatca gtaacctctt ttacccaagc acgccggttg caaaggcgtg
     6301 atcaagaaaa cgaaaaatta actcaacctg agagccgtac tttttctcaa actccagcgg
     6361 gtctgcatga agttcgttgt gatgttcccg gcacaacggt agcgtgaaaa tatcgtgggc
     6421 ctttgtcccc attcccccct gaccatgacc aatcaggtga tgcggatcgt cagcaggctt
     6481 accacaacac gcacacggct gtgtctttac ccagcgcgtg tatttctcat taacccaacg
     6541 gcgacgttta ggccgcttca tgaacgattc aggagactcc ggatcaacag cgatactgac
     6601 aaccgttttt ttctgtggtg gattttgttg ctggtggacg tgaagtggca gcgcaatatt
     6661 ttttgtgcgc tgcttcagta tgctgatggc tgtctgttct cccggtacga tgtcactctc
     6721 acggtatacg gagcggattt tttccgctgg taatcccagc gaacgacgcg ctactgcctc
     6781 aggtaatgca tccaccacct gattgcaggc cgcccaccag gataattcgg ccagcgataa
     6841 ctccctctcc tgcgtaccgc ttattgcatg acggatgacg tcaatcatcc aggcaaccag
     6901 attctgctga gcaagttgat cgagtgattc tgatgtctgg tcgcgcagct ggttgtcaca
     6961 gtgccagcac aacaccatcg cgccggtacc ataacggtga atgacggttt cgctgtgatg
     7021 ataatcgcca tgtggccact ggcaggattt cacgtgacgt aataaccagt cagacagcgc
     7081 accagcaccg ccagcggcac gaatcacccg ttcattgctg aaaaatggca gtaatgattt
     7141 atcctcagcc agcggctggc aaacgacagg aacaaccccg gacggcaaac tccgcatgct
     7201 tttgggttcc ggctccacca gcaccctgcc gcgatgaaaa accggcagta attcgcgacc
     7261 aggcttaatg accaccaggc caagttccgg gaccagaaca ggtcgaagta atacccgcac
     7321 attatctcca gacacgctga cggtagcagg catttgttcg tggcagatgt gcgcgaacag
     7381 gaagatatac agaaacggtc caggtcagac gatcaacgtt cagactccgc tccacacgga
     7441 caccgcgacg cagatacgcc tcttgaagca tatctgcctc atcggtcgta cagaacagat
     7501 agtgaaacca gccatactga ggcgtacgaa aacgccaccc ccgcttaatt tccgggtcgg
     7561 cttcagaatt gtgggatttt atgtgttgtg tcatcggatt ctccggtgac agcaggtgtc
     7621 agttgttcag gctgacggcg cgaattgtaa gtcaatacgc ctggaatgta caaacagaaa
     7681 acccgtcagt aagacgggct tagcaagccg ggacggttac tttaataatt tcagtgcctt
     7741 tacatcaact tcaacactgc tcaggtcttt atcaatttca ccctcaattc ttactttgtc
     7801 tttcggagaa acattctgcc cggcccatac gctgtcatca atatccgtga caattgtccc
     7861 gctattgtca cgaaactcat aacgttcatc acccactttt ttaatgatgc tcccttcaag
     7921 gataacccat gcatcatcct tcagttcttt tgcctgcgct actgttgaac gctctgcttc
     7981 aggcccctgg aaaccaccct gctgtgcaaa aacgccaaaa gacacaccgg aaataagtgc
     8041 tgcaatcaat acctttttca ttcatagtcc tctttcagaa atgaacattc aaacagcatt
     8101 ttcagtatgg taaagcgcgg gtgcgttgag gatgcctgac acatcagagg tggcgaggga
     8161 tttctccctc gcctggtcac tcttacttct cagattcgta gtctacgaag acagcaacct
     8221 ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt cctcgttacc agtgccgtca
     8281 caacaacggt aatacagatg acgatcaggg cgattaacat cgccttttgc tgcttcatag
     8341 cctgcttctc cttgcctttc ggcacgtaag aggctaacct acgtttgtga agcatagatt
     8401 gggcctcaga ttaatgttaa gcgtcctgca agacgcgtaa tgttaactgg ggcttttctc
     8461 tgtctgcctt acggcggcat gcccgaggca gacagcctca agcacccgca gcaattctac
     8521 ttaactcttc tttccccgca aaccgttttt atccccagcg gcaaatcgaa tacaccacca
     8581 gcgccaccgc cattgcaata ccaacatttg agaaggcctc aggccagctc attggcgcac
     8641 ctccttcggc ggttctggta gcggcatcca gtgtgtggct tcacacacga taggcgcacc
     8701 gaaacactgc gcatactgga agtcacccgt attatccagg ttaaaacctg taaactgttc
     8761 ccccgtgctg gacacaaaca gttgtacatc aacaccaact ggcggcattc gctcactaca
     8821 gcttatccaa ccatccggag ttaccggaga attgccagcc ttgcacatgg caatctccat
     8881 gatttcaacc atatctcctg gtggaatttt acaatgctga ccaatatgcc tctgctgcct
     8941 ggcatattcg aggatgtgct ccagtttggt tcgatcaatc atgatttatc tccattaagc
     9001 attgcagcac ggcaatcgtt ccatccttca gcataatcac tatgcgcaag aggccaacct
     9061 cttatgtatt cacgcggcga cttatcaggc actaccagcg ctggcgggac ggcaaataga
     9121 tatccgccaa agtcaggaag ctctccaatg gcctgtacga acttttgttt gcctacgtca
     9181 actcctaatg ggtaatgagc tataatcttt gccactggct ctgcttccag cgatgccagt
     9241 gcgataagcg ccagctcacg aatttcaccg ccgtctatat cgtcaatgtc atcgcggcca
     9301 gaaatgttag ccagccattg cagtcgctct ttggtaatag tggtcatctc actctccttc
     9361 tatgcgaata ccagcagcag tcagtgcggc attgacctca tcagcgtagt agtacgttaa
     9421 tccacttgat gatttagcca gcttgaatgg ttctggtaac ttcactatgc gggactcctg
     9481 ctcgtcgata cgctgttttg cttcctccag ttcatccagc aaatcagcga taatatccac
     9541 ttcacgatga cggatttcac gcttaaacgc agccaaagct gcatcacaat cctgctcagc
     9601 gtttggactg tctggtttct cctgatacca cgccagcatc gactgatagt tttgtgccgc
     9661 ctcacgcagc gcctgataat taatcccact cactggttgc ctcctggaaa ataactgcat
     9721 gccccagttt ctccgccagt gccagttctg ccttagcgcc tgccgaccgc tgccagcctt
     9781 tcagcatata aaccgcatcc acacaacgaa tcattgccat gcaaatatcc atgtaatgtg
     9841 gctgagtcag cccgtccgga agtactgacg ggtttaaaac ggtatgccct tcccgtttca
     9901 gtgcttcttc cgccttgtga aatgcctcgc ggttgaaatt ttcatatccc gtcattggac
     9961 cggcgatata aattctcacc ctcacgcctg aaccctcctg tcgaaataaa cgtagttatt
    10021 cactatgcgc agcggcatgc ctaattttct ggcaatttcc cttctttgca tgcctctctg
    10081 atgcagttgc cgcgcctgct caatatcacg ctgagaatat tttgctgact ggtgaaaatc
    10141 accacgtaac atcatgctga tgcccagctc ccgtgccttc gttctcactg ccgcttcagt
    10201 tcgtccgata agtgcgccaa tgctttttac ctttattgtt cccgcgcact gccggagtat
    10261 cagaatttca gcccggcacc acgtcttcca cccactcacc gctgctgttc tctggtggcg
    10321 gtaatatccc ggagaatatc ccggcacttg ttcagctccc gcagcgcggc gcagacacgc
    10381 tcccacttct gaacctgacc ttttgcccgg cgcagctcgc ggttggccac atgcagcgat
    10441 ggtaaaatca aaccatcctg atgctttctt gtgaacgacg gctgtgactg cactgtgacc
    10501 gccacacttt cagttttaat ttcttcctgt gtcgtcgttt ccttgactgg taacgcaaca
    10561 cctgctggct gaggaaaggc tttaccatcc gtttccgcta cggatgcagc ttccggctct
    10621 gccggtaaat cagcgcccgg tatgcagtaa cgaaatttac cgccctgatt cacgcgaatc
    10681 agacgccctt tgctgattgc catggccagc gatgaattcg cccggcggga ggtaatcccg
    10741 aacatcagtg ccagctcatc cgccgtttgt gggccatgtt gttcaatcgc ctcagtcagc
    10801 atttgcgctg tcactttcgg taccggtgac actggttcac tttcaccagc ctgaatcagc
    10861 caccacatcg accccttgtt atccgcttca ccacggcgct tcagtttcca cagttcgttg
    10921 accgcatctt cacggctgat tccaaggcgg gccgccacta cctgtgaaga ggcttttttc
    10981 agtgctttca gtgcgtcaaa tactgtttcc attaaaattt cctccgacaa aatcgtttct
    11041 cagattcaaa taaaaccagc tgccttccgg cgttcgtatt cctgtttcaa ccattcaatt
    11101 ggcgttggcc ctttcgggtg tttcgcccct tccagttgtc gtcgcactgg cggaacactc
    11161 atcccgttac caacatgctt tgtccatttc gtcagttgcc gttccgcaag tcgttttaac
    11221 tcaccctgcg tcatctggcg ctcaatccct ctggtacgca tttcgaggca gatgtggtac
    11281 agcacaggct gtggccacgg gtatttatca ctcccgtcgt atcgccagga ttcattgcgc
    11341 cagcgccggt actcttccat cacggcatcc accgtaagac caaatggatt tgtcccactc
    11401 tccgaaatca gcgcaacaaa ctcagccagg tccgggggcc acgtttcacc cgcccggcag
    11461 cggtccatgc actgacggca gacctgccgg atttgctgtt cagtcatcgc accaatctgg
    11521 gcaatccaga gcttcgaagg tgcggccccg ttcttctgag tccagcggtt cgaatacacc
    11581 tcccccataa gctcccacag cttccaggcc gtttccgttg ctgataaatc cgttgtcacg
    11641 ttcccactgt tcgcgtgctg cccggatttc ctgaactgcc cgtgatgccg tgccacctga
    11701 tgctgcatgg cttaccccct tgctgactgg ttttacctgt gccctgacgt gctgcacgtg
    11761 acgggcaaat ttctgctccc actgaacctg cgtgaaaacc ttcccctccg ccatccagta
    11821 atcccggaat gcggcaagct cagcaggtgt aaattccggc tcaggcagag ccatacccca
    11881 cactgctgcc cgttgtcgaa aatccggcga cggctgccag acagtagtca tcgaaaattt
    11941 cccgatgggt tcgctcaggc cgtccaggta ttcaggttcg gctgtctgca acggcgcacc
    12001 atgcgactca ctggtcggaa tactctcgcg ttcatgcacg ttatgtgtgg ggtttaattc
    12061 ttttagttct gtatctttat tagttgcttt tgtgtttgcg tcatgttcaa acacaacacc
    12121 aacatttgtt tgaacgcctg ttaaatctct ctcttgtttt gtttgaacat atgcttcctt
    12181 tctgcttctt ctggcctgaa cagatgcttt tccggcggct gattttttgg ttaatttttc
    12241 cctgactgat gccagatctt cctcaatccg aagatgcacc cattcatcgc cgttatcgca
    12301 aaaaaactcc cgcaaggatg gttccacatc agcccatcgc tcgttagtca gacgggaaat
    12361 ttttgccagc ctgtttttag gtattggttt ccctgtttgc cagtaattga acatcaacaa
    12421 caaatacgcg ccgtgctcct ctgcggacaa atgcatggtg tcagccaggt aatcagctat
    12481 gtacagttgc atgtatggta atgcggccat aattgcctca tcttgtgacg aaccatcctc
    12541 tggtgatatt ctgtgattcc ccaatcaaca gaatcagcag gggtctggca taaatatcaa
    12601 tgcaccacaa cagactcgcc ggatgacccg ctgtcgctga aatacgcttt ccggtaaacg
    12661 gcctggactg catcatcatg tgcatcaatt gccgtgctca acgcttcctg cgccgccagt
    12721 aatgcacggc gctcaacagt gtcaaaaata ctgaggtgat aacgcagttc acgcggaaga
    12781 acagtcagga tagccggaat tagtgcctga attttttcaa cagcatcagg cgtatccttt
    12841 tcaagccagc gaaaaatatt ttgtatgttc aatccaatac cctctgtcgt agaggtgtca
    12901 tgcattggtg gataagtcat ttcaagctca aaatatgctg tcgctatatc atcagcgatt
    12961 tttttgcgcc ctaccctcgg ataaagcagc cacgcattca ttgccatgcg gatatgttca
    13021 tgcttgattt tcatgaatca agctcctgga aagtggttgt gttaacgttt gggtatcttc
    13081 cagctcaggc caaatgttca tccaatcaaa aggccgtagt tgctgacgtg taacttcacc
    13141 attactggct cgctcaataa ggacacataa cgatgcccct aacacttgac ctttactcaa
    13201 tgcttttctt agataaccga tgctagtacc acactcacat gcaaacatac gctgttcatc
    13261 tgacgaaaga gaattgagaa atattcttaa ttcttccata a
//
LOCUS       DAZFVO020000132        13254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFVO020000132 DAZFVO020000000
VERSION     DAZFVO020000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.133"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..594)
                     /locus_tag="ACHKNB_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /pseudo
     CDS             complement(1..594)
                     /locus_tag="ACHKNB_RS18585"
                     /old_locus_tag="pgaptmp_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            656..1807
                     /locus_tag="ACHKNB_RS18590"
                     /old_locus_tag="pgaptmp_003718"
     CDS             656..1807
                     /locus_tag="ACHKNB_RS18590"
                     /old_locus_tag="pgaptmp_003718"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HGU4197154.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            complement(1817..2077)
                     /locus_tag="ACHKNB_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /pseudo
     CDS             complement(1817..2077)
                     /locus_tag="ACHKNB_RS18595"
                     /old_locus_tag="pgaptmp_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2453..2731)
                     /locus_tag="ACHKNB_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /pseudo
     CDS             complement(2453..2731)
                     /locus_tag="ACHKNB_RS18600"
                     /old_locus_tag="pgaptmp_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2858..2944
                     /locus_tag="ACHKNB_RS18605"
                     /old_locus_tag="pgaptmp_003721"
     CDS             2858..2944
                     /locus_tag="ACHKNB_RS18605"
                     /old_locus_tag="pgaptmp_003721"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197155.1"
                     /translation="MVKLALQPRGSVARIAQEHDINDNLLFK"
     gene            complement(3296..4552)
                     /locus_tag="ACHKNB_RS18610"
                     /old_locus_tag="pgaptmp_003722"
     CDS             complement(3296..4552)
                     /locus_tag="ACHKNB_RS18610"
                     /old_locus_tag="pgaptmp_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGU4197156.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(4565..4852)
                     /locus_tag="ACHKNB_RS18615"
                     /old_locus_tag="pgaptmp_003723"
     CDS             complement(4565..4852)
                     /locus_tag="ACHKNB_RS18615"
                     /old_locus_tag="pgaptmp_003723"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4197157.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            complement(4868..5311)
                     /locus_tag="ACHKNB_RS18620"
                     /old_locus_tag="pgaptmp_003724"
     CDS             complement(4868..5311)
                     /locus_tag="ACHKNB_RS18620"
                     /old_locus_tag="pgaptmp_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGU4197158.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            5582..6613
                     /locus_tag="ACHKNB_RS18625"
                     /old_locus_tag="pgaptmp_003725"
     CDS             5582..6613
                     /locus_tag="ACHKNB_RS18625"
                     /old_locus_tag="pgaptmp_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4197159.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKETEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEITLGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            7010..7279
                     /locus_tag="ACHKNB_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /pseudo
     CDS             7010..7279
                     /locus_tag="ACHKNB_RS18630"
                     /old_locus_tag="pgaptmp_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7510..7608)
                     /locus_tag="ACHKNB_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /pseudo
     CDS             complement(7510..7608)
                     /locus_tag="ACHKNB_RS18635"
                     /old_locus_tag="pgaptmp_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      7729..7917
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7982..9826
                     /gene="btuB"
                     /locus_tag="ACHKNB_RS18640"
                     /old_locus_tag="pgaptmp_003728"
     CDS             7982..9826
                     /gene="btuB"
                     /locus_tag="ACHKNB_RS18640"
                     /old_locus_tag="pgaptmp_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HGU4197160.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            9840..10031
                     /locus_tag="ACHKNB_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /pseudo
     CDS             9840..10031
                     /locus_tag="ACHKNB_RS18645"
                     /old_locus_tag="pgaptmp_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10172..12259)
                     /gene="iha"
                     /locus_tag="ACHKNB_RS18650"
                     /old_locus_tag="pgaptmp_003730"
     CDS             complement(10172..12259)
                     /gene="iha"
                     /locus_tag="ACHKNB_RS18650"
                     /old_locus_tag="pgaptmp_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HGU4197161.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSADKSTLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            12198..12380
                     /locus_tag="ACHKNB_RS18655"
                     /old_locus_tag="pgaptmp_003731"
     CDS             12198..12380
                     /locus_tag="ACHKNB_RS18655"
                     /old_locus_tag="pgaptmp_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197162.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHGNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            12800..12993
                     /locus_tag="ACHKNB_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /pseudo
     CDS             12800..12993
                     /locus_tag="ACHKNB_RS18660"
                     /old_locus_tag="pgaptmp_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
BASE COUNT         3653 a         3148 c         3043 g         3410 t
ORIGIN      
        1 gattagcccg gcaggtgggc gctggctcct gagcgccata aacagggctt tacctgtcag
       61 ctcttttgtc atgcgctctc ccatggcgta gccgacaatt tcgcacgtat aaacatcttt
      121 gatgccagcg aggtacaacc atccctcctg tgtggcaaca tacgtcaggt ccgccaccca
      181 gacctgattt ggtgctgtag gagcgaacgt ctggttcagc agatttggcg caactggcag
      241 attgtggttc gagttcgtag tcgctctgaa cttgcgtttc tgcttacagc gtagccttag
      301 ctccttacga agacgtgcca gtcggtcacg accaacgatg atgccattct ctgccagctc
      361 cgtctggagc cgccgggttc catatgtttc gcgagtgcgg atatgtgcca ccttaatctc
      421 cagttttagc cgctcatcac tttgttttct gtctgagggt tcatgctgta cccagttgta
      481 ataaccgctc ttggatacac caaatacctg acacatcgct tcaatgggaa attgttgtcg
      541 ccattgttcg attaacgcgt atttttcagc gactcctgtg caaaatacgc tgttgtagat
      601 tcaattggtc aacgcaacag ttatgtgaaa acatggggtt gcggaggttt tttgaatgag
      661 acgaacattt acagcagagg aaaaagcctc tgtttttgaa ctatggaaga acggaacagg
      721 cttcagtgaa atagcgaata tcctgggttc aaaacccgga acgatcttca ctatgttaag
      781 ggatactggc ggcataaaac cccatgagcg taagcgggct gtagctcacc tgacactgtc
      841 tgagcgcgag gagatacgag ctggtttgtc agccaaaatg agcattcgtg cgatagctac
      901 tgcgctgaat cgcagtcctt cgacgatctc acgtgaagtt cagcgtaatc ggggcagacg
      961 ctattacaaa gctgttgatg ctaataaccg agccaacaga atggcgaaaa ggccaaaacc
     1021 gtgcttactg gatcaaaatt taccattgcg aaagcttgtt ctggaaaagc tggagatgaa
     1081 atggtctcca gagcaaatat caggatggtt aaggcgaaca aaaccacgtc aaaaaacgct
     1141 gcgaatatca cctgagacaa tttataaaac gctgtacttt cgtagccgtg aagcgctaca
     1201 ccacctgaat atacagcatc tgcgacggtc gcatagcctt cgccatggca ggcgtcatac
     1261 ccgcaaaggc gaaagaggta cgattaacat agtgaacgga acaccaattc acgaacgttc
     1321 ccgaaatatc gataacagac gctctctggg gcattgggag ggcgatttag tctcaggtac
     1381 aaaaaactct catatagcca cacttgtaga ccgaaaatca cgttatacga tcatccttag
     1441 actcaggggc aaagattctg tctcagtaaa tcaggctctt accgacaaat tcctgagttt
     1501 accgtcagaa ctcagaaaat cactgacatg ggacagagga atggaactgg ccagacatct
     1561 agaatttact gtcagcaccg gcgttaaagt ttacttctgc gatcctcaga gtccttggca
     1621 gcggggaaca aatgagaaca caaatgggct aattcggcag tactttccta aaaagacatg
     1681 tcttgcccaa tatactcaac atgaactaga tctggttgct gctcagctaa acaacagacc
     1741 gagaaagaca ctgaagttca aaacaccgaa agagataatt gaaaggggtg ttgcattgac
     1801 agattgaatc tacagttgct ttttttaata tatctcgctc aaggcgagct tcatttaacg
     1861 ccttacgcag ttgcagaatt tcagattcca gttcagccac cgtgcgggaa ccaggagttc
     1921 cgagcccttt tctggcggcg gtaacccatt gtcctaaagt gccttcagga agagataatc
     1981 gggaagcgcc ttcactgatc gaaagttgat tttcaagaac cgttctgaca gcttcggctt
     2041 tgaactcttt agagtaacgt tgggtttttc tgctcattat tagctccttc tgatgccatt
     2101 ctatttcagg aaggagtgtc cgttaaactc aggctacctc attatgtggg atattgcact
     2161 tgctgcccaa tcatcattca gtcagcagaa gcaaaattaa accctgaagc agtcagacac
     2221 ggaatgagtg atcctcgtaa aagaattcag gaggtcagaa tgcgccaata cctgcatata
     2281 gaaagaggcc agctcactgc gtaaatcgtc atggggctaa aatccccgaa taagagtttg
     2341 taaaatccaa gatacagtct gactgtttca ggttctttaa tccgcgatat aacgaagctc
     2401 ccggagctgc gtaaggtctt tttgggctca atatttgaca aaggtcataa gagtttttct
     2461 ttccgagaca gactgcacga agcgctcttt ccgcagcatt attatccgcc tccgccagac
     2521 cgtcatcact gtaataacag agggcatccc actgattcag tacatagctg aacgcttcgc
     2581 ccagtctgga ttttttcgac agcgtaccat tcttctccac catccattca tgcagcgacg
     2641 tcagtaacac tttgcttcgc tgctgcctga cggaaagacg ctctgactcc ggtaatcccc
     2701 gtatttcgta agcgtaaacc gaccgccgta tgtagccatt aagccagtat tggtaacgta
     2761 ggtgcccacc ttttcaaact agtggacgcc tgttatggag caaaaaacat tatccgcaga
     2821 accccgcaga tcattttcaa atgagtttaa acttcaaatg gttaaactgg ctttacaacc
     2881 aaggggttct gttgcccgta tcgcccagga acacgatatc aatgataacc tcctgttcaa
     2941 atagctcaga ctctggcaaa acgaagggcg tatatctcgg cgtcttccgg taacaacctc
     3001 ttctgacact ggagttgaat tattacctgt ggaggtgacg ctggatgagc ggccttaccc
     3061 cgttttttac gcgaggtaat tatgaagatc aatgactagc cgtcgaaccg tgtgcatgaa
     3121 ctgctgcact gaaacttctc agctgtaaaa taatccttac gctacgtact tctcggagcg
     3181 cttacgatta atcggatgca gtcctgactg gaagaaagaa aaatgggaaa aaataagagg
     3241 aattaatatc ctccacgttc ctgaaatcct ggaggatatt aatgtcatca aaacattagc
     3301 gaacgataat ccctaacaaa atacccagtg cgccaaagtc agcatcactg aaggtggtgt
     3361 tagcaaaacc aatatcaccc agcacaggca ataagaatac tggcaggaag gtaataagca
     3421 agccctgagc gaaggcaccc aaaatcgcac cacgacgtcc acccgttgca ttaccgaata
     3481 cccctgcagc tgcacctacg aagaagtgcg gcaccacacc aggaataatc acgatcatgt
     3541 tcagcagata caacgtgaac ataccgatta aacctgcagc aaagctactc aggaaaccaa
     3601 ccagtacagc gttaggtgcg taagggaaga cgaccgggca atcgagagca ggtctggcat
     3661 tcggaaccag cttgtctgaa atacctttga atgccgggac aatctccgca atcaccatgc
     3721 gcacaccctg cagaatgatg tacacgccag ctgcgaaggt aatggattgc atgatagaga
     3781 acatgaacca gtttttacca ccgctaagct ctttcaccgc atctgcaccc gcaaacaggc
     3841 acgtcaccag gaaaattatg ctcatggtaa aagagatagc aaccggcgtg tcgcgcaaga
     3901 acagcagact tttcggcaca ttcatgtctt cagtcgagtg ctctttatta ccaaacttgc
     3961 tgccgatgaa accggccaat acataagata atgttgagaa gtggccgatg gcaacgtcat
     4021 cagaaccggt cactttcttc atgtacggat gcgcaatagc cgggaaaaag accattgcca
     4081 cgccaacaac caaagagcca acggctataa gcgtaatgcc ggtcatgcct gctgttgcca
     4141 gaattaccgc taccatcatc gacataaaca acgtgtgatg accggtcagg aagataaatt
     4201 tccacggtgt gaaacgggca atcataatgt tgataaccat cgcaaagaac ataatcatcg
     4261 ccatttcttt cccgaagctt ttctgcgcta cggaaacaat ggcttcattg ttcggcacga
     4321 caccctgaat accaaaggca tgctggaaaa tgtttgcgaa gtcacccagt gacgacacaa
     4381 ctaaacccgc ccctgcgcct aaaatcacaa accccataat ggtcttgacg gtacctttaa
     4441 tgcattcggt taccggtttt ttctgtgcaa tcaggccaat cagggcgatt aaaccgacca
     4501 gcactgcggg ttcggaaagt acatcgctca tcaaaaaacg aaagaaatcc atatcgcccc
     4561 cagcttacag tgcgcctaat tcggttaatg ccacggataa acgttctctc atggctttct
     4621 tatcaatcat attttccagt gcgacgattt tgcctgcgac agactgcgcg actaactgct
     4681 cggcaatgtc tttggtgccg acatagatat cactcggcgt ccctttcgcg gagcccaggt
     4741 cgacatgatc aacatcagcg ctgacggaca aatccttcaa aatgctttta atgctcattt
     4801 ccatcatcag gctggtgcct aagccgttac cgcaaaccac tgtaattttc ataaatgttc
     4861 ctcaggatta atagcgcgca ataacagaca aaatgtcctg cgtcgttttg gccgtcaaaa
     4921 tagcctgtat gtctttttcg ttatcgaata acttggcgag ttgagaaatc gcttcaatgt
     4981 ggctggtact gtcggttgca gccagcacaa tcagtaattt cactggatcg ttttcatcgg
     5041 catcaaaatt cacacctgaa gggatcaggg tcagtgctaa tgacagcttg ttagctccct
     5101 cttccgggcg cgcatgaggc atcgcaatac cagggccaac cacatagtac ggaccgatag
     5161 tgtcgtgcga gcgatagatc gcatcaacat aacgcggttc gatagaaccg ttttcaatca
     5221 acggccggca tgaaatcgcc acggcttccc gccagtcttt ggcctgttcc acgacctgaa
     5281 cgacctctgt atttaataga ttttttaaca tgcgactgat aactcccgat gataattaac
     5341 aggagagtag atattttacg cattaattaa tgtgatgatt gtcgcatttg atagcgctat
     5401 ctgataacgc taccaatctt tgtgatagcg ctatcatttc tctgtcgcca tgctttggct
     5461 atattcggtt cctgcatata caatgcaaca ggatatttgc taccgtgggc aaaaactgga
     5521 aagagtaatg gtaaaataaa agtaatgaaa acagctggtt gtaattatcc atggagtcga
     5581 aatggaaaaa acacgtaagc gccgtggtac cggacgggtg acgctgcagg aggtcgctaa
     5641 ctttgcaggt gtcggaacca tgaccgtttc ccgcgcgctg agaacaccgg aacaggtttc
     5701 tgacaagctg agggaaaaga tagaacaggc cgtagaagag ttgggttata ttcccaacag
     5761 aactgctggc gcccttgcat ctggtcacag tcatacagtc gccgtactgg ttccttccct
     5821 gacagataaa gccagctccc gctttatgca gtcgttgcag caggtgctta ataaaaatga
     5881 attccagcta ctgcttgggt gtcatgagta taatcagctc aaagaaactg aaattctgat
     5941 gacgcttctt caaggcaacc cggcggcgtt agttattttt ggttctcagt tggctgataa
     6001 aactcaccag atactcgaga aaacaaatat acctaccatt aacgttgtcg gttccccttt
     6061 cagtccggct caaattacca tcgaaacagc attttttgaa gcatcacata aactgaccga
     6121 acatctgctg gagcagggtt ataagaatat cggatttatc ggtgcgcata tggacaaccg
     6181 cctgcaacgt cagcaactga acggctggca taaagcaatg ctgagccatt accagaacac
     6241 tgatcttgtt atcaccatgc cggatgccgc aagtctgcaa ttggggcgtt acgcattaaa
     6301 cgagatatta ctgcgacaac cagagctgga tgctgtgatt tgcagccacg aagagattac
     6361 gctgggtatc atgtttgagt gccagcggcg tttgttaaaa atccctggaa atatcgctgt
     6421 agcttgtctt gacggttcag acaattgcga tcaaacacac ccaactttga catccatccg
     6481 tattgactat aaaaaaatgg ggacagagac gggtaagctg ctgattggac tattgaataa
     6541 taatcatgat gaatcagaag agtcgcgtat cgtgcaattc aactaccaga tagaattacg
     6601 tcagagtaca tgagcctaaa agagcactat tacaaagtta tctattgtgg tagggatagc
     6661 cgtttccggc tattccctgc gcagttccgg atgtatgctg ctaacgcatc cattcctact
     6721 tcaggtattt ggcgtagaag cggatgctaa aaacttgccg caaaaccgca atatcagcct
     6781 gcagtatcgc tctccagatt atccattcct atattgatga caacgacagg taagaccgtg
     6841 acctgataaa cctgaataag ctcctggaca taaagtccgt aatgggaata aggcgagagt
     6901 cagcgaatct catcgaggcc agcagcactg gctgcaataa ggatatagag cagcaatcta
     6961 ccgtcaacgt caaaaacacg gaagccttgc aaacgggatg cgttcatata aacaacatgg
     7021 agcagcaaca atacgatgcc tgtcgttgct cagtcaggac cttttctgtc atgcgcgagc
     7081 cagcgtcacg attgataaat aatgttagca actgattaat ttactgcaac agacgtggtt
     7141 ggtaataagc actggcggac aatgcaggat ggaaggctgt tttcaggttg tccgttcagg
     7201 tacgttagat cagttcgttg cgctaaagac aactgttcag tcccttatgt tacccgttat
     7261 ggcaaccatt gcccggtgat aaccgggcgt gtcatctgtg catccgttga ttcgtgtgga
     7321 ctgataaagt tttgtctgct gaatctttat acggaatgct cttcagtatg cgtaagataa
     7381 tttgtttttt ggaggattgt cgacaatcag agggtaaaca aaagaatggt tgaatgtgga
     7441 tgaggttcat attattgatt cagatttttt gaagaatcac cacatgggat gtccatgtgg
     7501 ttgtataact cagggccagc gcttaataat gatggatgta ttaatgccgc caaaggcaaa
     7561 gttgttactc tgcaaaaaca cacaatcaac cttgcgggct tcatgcaact aattgagcat
     7621 atatgtttta ttcttattat ggtcgccgat aaaatattat gtgtattaga tccgagtgtt
     7681 catgcattcc tatttttgag tcatgtatgg acagtaactt gttacaacca gtaatatcca
     7741 gttgtcggtc ctgcgagtta atagggaatc cagtgcaaat ccgaaactga cacgcagcgg
     7801 taaggaaagg tgcgatgatt gcgtaatgtg gacacggtca tccggtgggt ggtcatctct
     7861 ttgtatcttc agatgccctt ctaggtctga agacctgtcg gttaacgtcg catctggttc
     7921 tcatcatcgc ttactatcga tggtaactgc gatattcttc ttctgttgtg gatgcgttac
     7981 aatgattaaa aaatattggt ttctaatggt gtactccgtc acagcctttt ctgtctgggc
     8041 acaggatacc cctctggata ctctcgttgt tactgctaac cgttttcagg aaccattaag
     8101 taccgtgctt gctcctgtaa ctattgtgac acgagaagat atagatcgct ggcaagtttc
     8161 ttctgtaaat gatgttctgc gtcgtttgcc tggcgttgca atttctcagc atgggggaga
     8221 agggcagctc tcaacaattt ttgttcgtgg gacaaactct aaccacactc tggtattgat
     8281 tgatggagtg cgcctgaatt tagcgggtgt cagtggagct gcagacctga gtcagtttcc
     8341 cgtcgcttta gtgcagcgta tagaatatat ccgtgggcca cgttcagcaa tttatggttc
     8401 agatgccatt ggcggagtga taaatattat tacatcccgt gagaatactg gcactgaaat
     8461 ttccgcagga tggggaagta atagttatca acattacgat atatccactc atcagcaact
     8521 tggggaaaat acccgtgtaa cacttcttgg cgattatacc tatactaggg ggtttgatgc
     8581 tgttgcctat ggcagtacag ggatgcaacc acagtcagac agagacggtt ttttaagtaa
     8641 aacattctat ggcaaacttg aacataactt atctgatacg tggagcggat ttgttcgtgg
     8701 ctacggctat aataaccgaa ctaaatatga cgcctggtac tctcccggtt ctccactgat
     8761 tgatactcga aaactttata gtcaaagctg ggatgcaggt cttcgctacg ctggtgaaac
     8821 actgcaatct caacttgttt ccagttatag ccatagtaaa gattacaact acgatccaca
     8881 ttatggacgc tatgacacat cagctaacct ggatgatatg aaacaataca atctccagtg
     8941 gacaaatagc gtcacagtcg gccacggtaa tgttggcgct ggtatcgact ggcaaaaaca
     9001 atccactact cctggaactg gatatctgcc aaaaggttac gatcagcgta acacggggat
     9061 ttatctgaca ggactgcaaa aacttggcga ttttacgtta gaaggcgcgg tgcgtaatga
     9121 cgacaactcg caatttgaac gccacacaac atggcaatcc agtgcgggat gggagtttat
     9181 tgagggttat cgctttatcg cttcctatgg aactgctttc aaagcgccaa atctggggca
     9241 gctatatggt atgtatggta atccgaatct ggctccggaa aagagtaaac agtgggaagg
     9301 ggcatttgaa ggtttaactg ggggcgtaaa ttggcgtatt tcaggatacc gtaatgatat
     9361 cagtgaaatg attaactata atcctcatac ccttcgttat tacaacgacg gaaaagtaca
     9421 cgttaaagga attgaggcta cagtaaactt tgatacagga gcactgacgc atactgttag
     9481 ttacgattat acggatgcca gaaatgcctt aactgacaaa ccattggaac gtcgtccaaa
     9541 actgcaagtg aagtatcaac ttgactggca agtattcgat tttgactggg ggataactta
     9601 tcaatatatg ggatcgcgat acgattcgga ctactcatcc tggccctata agtcagtcaa
     9661 aatgggcggt gtgagtttat gggatgttgc ggttgcgtat ccggtcactc ctcacctgat
     9721 agttcgcggt aaaatagcta atctgttcaa caaagattat gagactggtt atggctatca
     9781 ggcagcagga cgggaatata tattgtctgg cagctatact ttctgaaatc tgccccaccg
     9841 agtcgctaag cagcgtgatc cgccatgcga tcaaaaagcg taaagtgttc ccgacagacg
     9901 aggcggtgaa aaaagttgtg tggctggcaa tccagtctgc gtcccagaaa tggacgatgc
     9961 cgttgaagga ctggcgtatg gaaatgagcc gctttattat caagttcagt gaccgcctgg
    10021 acggtcactt ctgggaaaag gcatttacac agaatcctga acaggctcgg tcacttcgac
    10081 cagacaccgt gcgtcatgta acgttgccac tgttctgcca ggagttacag tggcaacgta
    10141 tgctaccgtc agtgatggcg ttttgttgtt atcagaactg atagttcagc gacatccagt
    10201 aatttcgctc aggtatcaca tatcccgttg ttgatgatcc cgtctggaag taatcaccgg
    10261 cataaagcgt actcttatcg gcactgtaca ggctcacgtc actgtaatcc ttgttgagca
    10321 ggttattcac cgcagcattc agcgtcaggg catccgtcac cttccacgac agacctgcat
    10381 ccaccaccgt ccaggctttc agatattctc ctttctcatc atacactttc ttctgtacag
    10441 cgctcagcga cgaataattc cgggtgaaac gtggtgtttt cccgcgataa cgggcaccca
    10501 gccatgatgc cacctcttcg gtgatctgcc agttcagttt cgcattcacc atgtgttcag
    10561 gggtataact cagcggtgca cctttgttat caccatcacg ttgttcactt tgggtccagg
    10621 tgtaattcaa cgacagagtg acatcctctg accacagcgg caatgtgccg gcgaattcca
    10681 caccatgcaa gcgggccttt ccgctgtttg tatagctatt ggtgttatca tcaatggaat
    10741 aagagacaat cttgttggag aagtctgtta taaagcctgt tatattggca ttcagaccgg
    10801 agaggttatc gtaatatacc cccgcctcat aacttacact ctcttccggc ttcagttcgg
    10861 ggttaccgag tagattagta cgtccctgcc cggatacacc gctgatcccc ttatgtaact
    10921 gtcccatggt gggggcttta tatcccgtgg tcacaccgcc tttcagcgtc caggcatcgg
    10981 tcacgtccca gaccagatat gcacgcggac tgaagtgtcc cccgaattga tcatgatgtt
    11041 catagcgact gcctgcagtc agcgcaagta catccgtgag atgccactca tcctcagcaa
    11101 atactgacca gcttttctgt cggaaagttt cacctgtgct ggcaaggaca accccatctt
    11161 tcatggacga actctgatac tctcccccca cagtaagcag atgagattct cccagagggg
    11221 taagcagcaa tgaattcagg ataaggttag attccttaag ctcccgcggt ttaccggcaa
    11281 gcccccattt atcgctcttc agaacactac gtacaagctc acgaccttta ttttccgtct
    11341 cgttccagtt cagatacgat ttccaggtac cgaaggtgaa agtatgatca tagccagccg
    11401 aaattttgtt tcgctcatag cgcagagtat ggtcatatcc ccctgtcagg ctacccagtt
    11461 gaccatcctg gttatcgtaa cgctgtcggg tggtatccat atcaaaccag agcacatcct
    11521 gctccgtagc cttccagtca atacgcgcac caagattata attctgtgac tccgtgggat
    11581 aaggaatacg cgtggctgat gtgtcgctca gtgatgtgac cgatgagccc tgacgctgct
    11641 gtgtgctgcc gcggatctgc agactgacag aatcatccac aagagggcca ctgctccaga
    11701 aattaaactg gctgctgttg ccccatttgt tactctcctg cagattcagc ccggcgttga
    11761 cggaagagag ccatttgtct gcattctttt tggtaatgat attcaccaca ccacccatcg
    11821 catcagagcc atacagtgtg gacatcggcc ccctgataac ctcaatacgc tcaatcgcgg
    11881 ccagaggagg catgaaccct gtattcatgg cagaaaaacc gttaggcgtc acgtccccgc
    11941 tcccgccctg acggacacca tcaatcagta tcagcgtgta actggcgggc attccacgga
    12001 tgctgatttc cagcccaccg gttttacccg taccactttc aacatccaca ccctccacag
    12061 ctctcaatgc ctccgccaga tcgtggtact ggctggactg caattcctcc tgactgatta
    12121 cggaaacact ggcggctgcg ttggtcagtt ttttctcata gccggatgcc gagacaatca
    12181 tcacatcctc tgctgccatg ctgctggctg aaaacccgag acagggaatg acaacggaag
    12241 ccagagtggt tattcgcata ttactaatct ccgatcatgg taatcggcaa gacaatacac
    12301 aaatacaaac aataatgaga ataatttata tttacctttt acaaaaaatg aatattactg
    12361 acaaaactaa atataaataa tgactgcctc ggcatgccag ttaacagcca tccgcaatgg
    12421 cgtgttcgat tttgcgaata aaaaatggtt actgtagaag cggattttca ccacctgtca
    12481 accatgaatg gtcagtggag tgagacgact ggtatgattt ggtcgatgac ttcttccggt
    12541 gaatacggac agaaacattg aaatattgta agagagaacg ttcagaattc tgtgtcacgt
    12601 gaaaatacta cagcgttatc accttattca gtcgaccttc aaaataaacg tctaaccgtg
    12661 acaatcacca tttggattgc ccactggaga ggcgaacgat tgcctcgacg gcatttataa
    12721 tgccagccca cattgttggc gctgacaata ctaaggacaa acgatgaggg agaaccgtct
    12781 tataccacta gcgctgacac cggcacacct tccccgtgaa atacaccggc tggagcctga
    12841 agaaagctgt tgcccggagt gtggcggtga gctggattat ctggggaagt cagcgctgaa
    12901 caactggaac tggtgagcag ctccctgaaa gtgatcagca cagaacgggt aaaaaaagcc
    12961 tgtaaaaaat gtgacggtac cgttgaagcg ccggtctcgt aagaataatc ctgggcaaat
    13021 aagatttttc ctgaaaaagc gtctagtctt ttgatatcgt tgtttttatg ctaatcaggg
    13081 agtatatgtg atgattgtct gttacgttat tcttgttaac cgtccgtttg aaacagagat
    13141 tgccgggaaa aagctatttt ttcagaaaaa tgacgtcctg ttgacagata acatcagcca
    13201 gtcctttttc ctgcgccatc ccggtaatgt tactgagctc cggattgacg aatg
//
LOCUS       DAZFVO020000133        13248 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFVO020000133 DAZFVO020000000
VERSION     DAZFVO020000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.134"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..545
                     /locus_tag="ACHKNB_RS18665"
                     /old_locus_tag="pgaptmp_003733"
     CDS             <1..545
                     /locus_tag="ACHKNB_RS18665"
                     /old_locus_tag="pgaptmp_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4197163.1"
                     /translation="QGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCD
                     VPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAESGLS"
     gene            complement(587..1357)
                     /gene="yafV"
                     /locus_tag="ACHKNB_RS18670"
                     /old_locus_tag="pgaptmp_003734"
     CDS             complement(587..1357)
                     /gene="yafV"
                     /locus_tag="ACHKNB_RS18670"
                     /old_locus_tag="pgaptmp_003734"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HGU4197164.1"
                     /translation="MPGLKITLLQQPLVWMNGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            1538..1984
                     /gene="ivy"
                     /locus_tag="ACHKNB_RS18675"
                     /old_locus_tag="pgaptmp_003735"
     CDS             1538..1984
                     /gene="ivy"
                     /locus_tag="ACHKNB_RS18675"
                     /old_locus_tag="pgaptmp_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HGU4197165.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2027..4471)
                     /gene="fadE"
                     /locus_tag="ACHKNB_RS18680"
                     /old_locus_tag="pgaptmp_003736"
     CDS             complement(2027..4471)
                     /gene="fadE"
                     /locus_tag="ACHKNB_RS18680"
                     /old_locus_tag="pgaptmp_003736"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HGU4197166.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4711..5289
                     /gene="lpcA"
                     /locus_tag="ACHKNB_RS18685"
                     /old_locus_tag="pgaptmp_003737"
     CDS             4711..5289
                     /gene="lpcA"
                     /locus_tag="ACHKNB_RS18685"
                     /old_locus_tag="pgaptmp_003737"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HGU4197167.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5495..6262
                     /gene="yafJ"
                     /locus_tag="ACHKNB_RS18690"
                     /old_locus_tag="pgaptmp_003738"
     CDS             5495..6262
                     /gene="yafJ"
                     /locus_tag="ACHKNB_RS18690"
                     /old_locus_tag="pgaptmp_003738"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HGU4197168.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6233..6973)
                     /gene="dpaA"
                     /locus_tag="ACHKNB_RS18695"
                     /old_locus_tag="pgaptmp_003739"
     CDS             complement(6233..6973)
                     /gene="dpaA"
                     /locus_tag="ACHKNB_RS18695"
                     /old_locus_tag="pgaptmp_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HGU4197169.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPNSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7129..7407)
                     /gene="yafQ"
                     /locus_tag="ACHKNB_RS18700"
                     /old_locus_tag="pgaptmp_003740"
     CDS             complement(7129..7407)
                     /gene="yafQ"
                     /locus_tag="ACHKNB_RS18700"
                     /old_locus_tag="pgaptmp_003740"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HGU4197170.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7410..7670)
                     /gene="dinJ"
                     /locus_tag="ACHKNB_RS18705"
                     /old_locus_tag="pgaptmp_003741"
     CDS             complement(7410..7670)
                     /gene="dinJ"
                     /locus_tag="ACHKNB_RS18705"
                     /old_locus_tag="pgaptmp_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HGU4197171.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7856..8629
                     /gene="yafL"
                     /locus_tag="ACHKNB_RS18710"
                     /old_locus_tag="pgaptmp_003742"
     CDS             7856..8629
                     /gene="yafL"
                     /locus_tag="ACHKNB_RS18710"
                     /old_locus_tag="pgaptmp_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4197172.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGEIIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            complement(8686..9042)
                     /locus_tag="ACHKNB_RS18715"
                     /old_locus_tag="pgaptmp_003743"
     CDS             complement(8686..9042)
                     /locus_tag="ACHKNB_RS18715"
                     /old_locus_tag="pgaptmp_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGU4197173.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(9035..9313)
                     /locus_tag="ACHKNB_RS18720"
                     /old_locus_tag="pgaptmp_003744"
     CDS             complement(9035..9313)
                     /locus_tag="ACHKNB_RS18720"
                     /old_locus_tag="pgaptmp_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGU4197174.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9418..11130)
                     /locus_tag="ACHKNB_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /pseudo
     CDS             complement(9418..11130)
                     /locus_tag="ACHKNB_RS18725"
                     /old_locus_tag="pgaptmp_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11102..11887
                     /gene="lafU"
                     /locus_tag="ACHKNB_RS18730"
                     /old_locus_tag="pgaptmp_003746"
     CDS             11102..11887
                     /gene="lafU"
                     /locus_tag="ACHKNB_RS18730"
                     /old_locus_tag="pgaptmp_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HGU4197175.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11958..13013
                     /gene="dinB"
                     /locus_tag="ACHKNB_RS18735"
                     /old_locus_tag="pgaptmp_003747"
     CDS             11958..13013
                     /gene="dinB"
                     /locus_tag="ACHKNB_RS18735"
                     /old_locus_tag="pgaptmp_003747"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGU4197176.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            13010..>13248
                     /locus_tag="ACHKNB_RS18740"
                     /old_locus_tag="pgaptmp_003748"
     CDS             13010..>13248
                     /locus_tag="ACHKNB_RS18740"
                     /old_locus_tag="pgaptmp_003748"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4197177.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYID"
BASE COUNT         3255 a         3482 c         3374 g         3137 t
ORIGIN      
        1 accaggggcg gctaaataaa gcctttgagg aaaaatttcc gctgaaagaa ttaaataatc
       61 cagagcatga cagttacgca atgagtgaaa agagtcacgg cagagaagaa atccgtcttc
      121 atattgtttg cgatgtccct gatgaactta ttgatttcac gtttgaatgg aaaggactga
      181 agaaattatg cgtggcagtc tcctttcggt caataatagc agaacaaaag aaagagccag
      241 aaatgacggt cagatattat atcagttctg ctgatttaac cgcagaaaag ttcgccacag
      301 caatccgaaa ccactggcac gtggagaata agctgcactg gcgtctggac gtggtaatga
      361 atgaagacga ctgcaaaata agaagaggaa acgccgcaga attattttca gggatacggc
      421 acatcgctat taatattttg acgaatgata aggtattcaa ggcagggtta agacgtaaga
      481 tgcgaaaagc agctatggac agaaactatc tggcgtcagt ccttgcggag agcgggcttt
      541 cgtagtctta ccccgactct cccccagcct taaacacaac ccccactcac cgcaacctaa
      601 actcatccgc atcccgccat gccggaaact tatcgcgata ttcccttaac accgccatcg
      661 acagctcggc atcaatgcgc gttgcctgat gcgcgtcggc agtagcgata atctcgcctt
      721 gtggattaat cacccggctg tcaccgcgat aatggcagcc gttgccatcg ctgccgacgc
      781 gattgcatcc cgccacatac gcctgattct caatcgcgcg ggccgtcagc aatgcctgcc
      841 agtgcagaga gcgcggagca ggccagttgg cgacgtacag ggcgaggtca taatcgttga
      901 gattgcgcga ccacacagga aaacgtaagt cgtagcacac cagcggcaaa atacgccagc
      961 cgcgccattc cacaatcact cgcgcattgc ccgctttata atgtagatgc tcatctgcca
     1021 tgcggaacag atgacgctta tcataaaaat gtaccgtgcc gcccggctca accagcaaaa
     1081 agcggttaac cgaaccagac tccgtttgta atgcaacact gcctgcaatc agcgcattgc
     1141 actgctgcgc cttagctgtc atccagttca ctacgtcatc ttgtgctagc gacgaagctg
     1201 ccgcttccat ggcaaagccg ctggtaaaca tctccggtag aacgatcaca tcgcgcccgg
     1261 taataccttc cagttgacga tcaaaatgac gcaggttggc agggccattc atccacacca
     1321 gtggttgctg caaaagcgta atcttcaaac caggcacggt gtacaactcc tttatgcgaa
     1381 gggttttata actttaacac cttatcaggc agttgcctta gcgcagaata aattgataac
     1441 aaatgctgat attggaaata tctgatttgc aaattatcgt gttatcgcca ggctttagaa
     1501 ggttaataac atgggcagga taagctcggg aggaatgatg tttaaggcaa taacgacagt
     1561 cgccgctctg gtcatcgcca ccagtgcaat ggcgcaggat gatttaacca ttagcagcct
     1621 tgcaaagggc gaaaccacca aagctgcatt taatcagatg gtacaagggc ataagctgcc
     1681 tgcctgggtg atgaaaggcg gtacttatac tcccgcacaa accgtaacgt tgggagatga
     1741 gacgtatcag gtgatgagcg cgtgcaaacc gcatgactgt ggctcgcaac gtatcgctgt
     1801 gatgtggtcc gagaaatcta atcagatgac ggggctgttc tcgactattg atgagaaaac
     1861 gtcgcaagag aaactcacct ggctgaatgt gaacgatgcg ctttcgattg atggtaaaac
     1921 ggtgctgttc gcggcgttga ccggcagcct ggaaaaccat ccggatggct ttaattttaa
     1981 ataattagcg gataaagaaa cggagccttt cggctccgtt attcaattac gcggcttcaa
     2041 ctttccgcac tttctccggc aactttaccg gcttcgtcgc cagctcttcc ggatcaaagt
     2101 catcaacgtt aatactgcgc agacggcttt cttcagcttt caccagaata gcggcttcat
     2161 ctttatcaat cagccccttc gccagcgcgt tgtgcgccag ttcatccaga cgggtaaacg
     2221 gcaggttttt acccagctct ttacagatcc gctgatgaat tgggtcggcg gcaatcacat
     2281 ccaccagcgc ctcttccagc aagccaaccg gattatgctc gctcggcgtc aggtactgac
     2341 cgcgaccaat gcgggaacgg gtggcgttcg gcacttgtaa aatcttcgcc actttatgat
     2401 ccagcttgtc agaaggtgcc agataatgac gtccggtcgg gaagatcacc acattcagca
     2461 gcccggcaac cacgcggttc gggaagtttt gcagtaaatc atccatcgcc tgttcagcct
     2521 gatacagcgc atcttgtacg ccccagtgca ccagcggcag gtcggcttca ttacggcctt
     2581 cgtcgtcata acgcttcaga acggcagagg cgaggtaaag ctggcttaaa atatccccca
     2641 gacgtgccga gatgcgctca cgacgtttca ggctgccgcc cagcacagcc atcgagacat
     2701 cagaaagcag ggcgaggttg gcgctcaggc ggttcaggtg ctgatagtag cgtttagtgg
     2761 catcgccggt tggcgtgctg ctggttaaac cgcgcgtcag gcccagccag aagctgcgaa
     2821 ctttgttgct accgacgtga ccgatatgtt tgaacaacag tttatcgaac gcgttgacgt
     2881 cattgttctt cgctgcttcc atctcttcca gcacgtacgg atggcaacga atcgctcctt
     2941 gtccgaagat catcatgctg cgggtcagaa tgttggcccc ttcaacggtg atggcaatcg
     3001 gtgcgccctg gtaagcacgc gccaggaagt tgctctgccc gagcataatg cctttgccac
     3061 cagtgatatc catcgcatca ataatcgact gctgcccgcg gtgggtacag tgatacttaa
     3121 cgatagccga cagcacggca ggtttttcgc cgagcataat gccgtaggta atcagcgatg
     3181 ccgcagcatc catcacgtag gcattaccgg caatacgcgc cagcggctct tcaatccctt
     3241 ccatcttacc gatagagatt ttgaactgac ggcgaatgtg agcatacgcg cccgttgcca
     3301 gcgctactga tttcacgccg ccggttgagt tggaaggcag ggtgatgccg cggcctaccg
     3361 agaggcactc caccagcatc cgccagcctt gcccggccat tttcggcccg ccgataatgt
     3421 aatcgatcgg tacgaagaca tctttaccgc gcgtcggtcc attctggaac ggcacgttca
     3481 gcgggaagtg gcgacgacca atttccacgc ccggcgtggt ggttgggatc agcgcacagg
     3541 taatacctaa atcttctgcg ccacccagca atttctccgg gtcggagagt ttaaacgcca
     3601 gcccaagcac ggtcgcaatc ggtgccagcg taatgtagcg tttgttccag gtcagacgca
     3661 tccccagcac ctgctggccc tgccattcgc ccatgcagac aatcccggta tccgggatag
     3721 cgcctgcatc ggaacccgct tccgggctgg tcagtgcaaa gcaagggatc tcctgaccac
     3781 gcgccagacg cggcagatag tgatttttct gctcgtcagt gccgtaatgt tgcagcagct
     3841 cgcccgggcc taatgagttc ggcacgccga cggtaatcgc caggatcccg ctcacgccgg
     3901 agagtttttg cagcacgcga gactgggcat aagccgagaa ctccagcccg ccgtactctt
     3961 ttttgatgat catcgcgaag aaacgatgct ctttaaggta cgcccacaac tccggcggca
     4021 aatcagccag ctcatgggtg atctggaaat cattcgccat ccggcaggct tcttctaccg
     4081 gaccgtcgag aaacgcttgc tcttctgcgg tcaggcgcgg ctgcggatag ttatgcagct
     4141 ttttccagtc cggcttgccc tggaacaagt cgccctccca ccaggtggtg cccgcatcaa
     4201 tcgcttcttt ctcagtgcgc gacatcggcg gcatcacctt acggaaaccg cgaaataccg
     4261 gcgcggaaat catcgactta cgcataggcg caaagttaaa tggcacgagg ataatggcca
     4321 gaggcaccag tacccacgcc gaccacagac cagcaacgcc gagggcggct gtccaggcga
     4381 gcaaaatcag actgctgata aataagctca cgcggtgata gaacaacgcg ccgagcagga
     4441 caaccgtagc gagaatactc aaaatcatca taacgaaaag ccccttactt gtaggaggtc
     4501 tgaccacttg tgatgatatg gttgtagtgg atgtaaaaac atttagcaat gtgtttacaa
     4561 tataattaca acaaagctca cattgttgct gtttttatcc gcacttcagg tcaaaaagtc
     4621 ctggtcatag cacctgcccg tacttctcgc ttttggcggt atccggtaca ctgcattttg
     4681 tctattacat ttatgctgaa ggatatcctc atgtaccagg atcttattcg taacgaactg
     4741 aacgaagcgg cggaaacgct ggctaacttt ttaaaagatg acgccaatat tcacgccatt
     4801 cagcgcgcgg cggtcctgtt agcagacagc tttaaagccg gtggcaaagt gctttcctgc
     4861 ggcaacggcg gttcccattg cgacgctatg cactttgccg aagagttgac cggtcgctac
     4921 cgtgaaaacc gtccgggcta cccggcgatt gctatttctg acgttagtca tatttcatgc
     4981 gtcggtaacg atttcggttt caatgatatt ttctcccgtt acgttgaagc ggtaggtcgc
     5041 gaaggcgatg tactgctggg gatctccacc tccggtaact ctgcaaacgt gatcaaagcg
     5101 atcgcagcgg cgcgtgagaa ggggatgaaa gtgatcaccc tgaccggtaa agacggcggc
     5161 aaaatggctg gcacggcgga tatcgaaatt cgcgtaccgc actttggtta tgccgaccgc
     5221 attcaggaga ttcacattaa agtgatccat atcctgatcc agttaattga aaaagagatg
     5281 gttaagtaag tctggcgtag gctggataag gcgtttacgc cgcatccggc acttgtgcgc
     5341 tgatgcctga tgcgatgctg acgcgtctta tcatgcctac aaatctgtac ccgaaccgta
     5401 ggccgaataa ttcgtttacg ccgcatccga cctgaaaatt cttaaatcaa tcttcgccgg
     5461 gggccattcg ctcccgctgt tgtggaggtt acccatgtgc gaattgctcg ggatgagcgc
     5521 caacgtccct accgatatct gctttagttt caccggactt gtacagcgtg gtggtggaac
     5581 cgggccacat aaagatggct ggggcattac cttttacgaa ggtaaaggct gtcgcacatt
     5641 taaagatcca caacccagct ttaattcccc catcgccaaa cttgtccagg actacccgat
     5701 aaaatcctgt tcggtggtgg ctcatattcg ccaggctaat cggggcgagg tggcgctgga
     5761 aaatactcac ccatttaccc gcgagttatg ggggcgtaac tggacttatg cccataacgg
     5821 acaactgacg ggctacaaat cactggaaac cggcaacttc cgcccggtcg gtgaaaccga
     5881 tagcgaaaaa gccttctgct ggctcctgca taaattaacg cagcgttacc cgcgcacacc
     5941 gggcaacatg gcggcagtgt ttaaatatat cgcctcactg gcggatgaac tgcggcagaa
     6001 gggcgttttc aacatgctgc tttctgacgg gcgctatgtg atggcgtatt gctcgactaa
     6061 tttacactgg atcacccgcc gcgcgccgtt tggcgtggca acgctgctgg atcaggatgt
     6121 ggaaatcgac ttcagctcgc agaccacacc gaatgatgtg gtcacggtga ttgcaacaca
     6181 gccgctgacg ggcaatgaaa cctggcaaaa gattatgcca ggcgaatggc gcttattttg
     6241 cctcggggag cgtgtagttt gatgccagtt gtggctgcac aacttcgtgg cttaacggct
     6301 tgctgaccac gtaacgacca ttgaccacag aaacggtcgg tggcttacgg gtttgctcaa
     6361 agtagtcgta acccggcttc agttgctccc agaagtcctt aaagttggaa tatttatggc
     6421 gcttcatatt ggcgtcggtc atgcggaacg ggtaaatact cacctgcacg ctcggctgac
     6481 caaacaccag cgcaccagta acgaactgga agatctcatc aataccctga ttggtcattg
     6541 cgtagcagcc gatggaaaca caatcgccgt ggatcatcag gtatttccct tcataaccat
     6601 gcgcacggtc ataggcattg gggaaaccaa tattaatcgc tttgtagtaa cggctgtttg
     6661 gttttaactg attacgctgg acgctataaa acccttccgg gcttttgaaa tcgccctgac
     6721 gctgttttgg ccctaagccg ccggaatatt tacagatttt ataactgtcg agcagctgat
     6781 attgctcgcc cattttgacg tagagatcga gcgtacgttc ttccttgaag atctggatgt
     6841 agacagggga tcccatcaac tgctgcttat actctttgct cactggcgtg gtcgagctac
     6901 tgctgcccag caaaccggca aacgaaacgc acgggatcaa caacatcgca agaattaatg
     6961 cgattttacg catactgctt attccttgat aaaacggtta cacacgccag gacggcaaaa
     7021 tagatcccaa atcggaatag tctggatttg gaaggctcac attatcacca aaagagtttt
     7081 acgcaagcct gtcggcgcag ggtttacaaa tttcatcggc agcgtgcttt acccaaagag
     7141 cgccgcgtga gttccagttc tctcaaatcg taaaagttta tcggtaagtt tgtaaatcag
     7201 gatccagtcc ggttcgacat gagcatcgcg ataacctttc catgaacctt gcagcgggtg
     7261 gtctttataa acagctggaa gcggtaaagc attattgata agaagcgtca taagatattt
     7321 caatttattc atatccttat gacgcttttg tgcaagtttt acatcctttg aaaattgtcc
     7381 cgagtattca atatcccttt gaatcatatt taaattccta atttatcaaa taaatcatcg
     7441 gcgtctttgg ccttatgaac atcaacgcca gcttcgctgt ttttgattga ttgaatggtt
     7501 aattgattag gctcgcgtaa atcaaacggc aatgcctttt cacgcgcgac ctttgtgagg
     7561 gttatgcgaa ccaggtcaga gatggtcagc cccatcccgg ccagtacgtc cgccgcctga
     7621 ttcttcagat cttcatcgat tcgggcgcga acaaatgcgt tagcagccat tggggtatct
     7681 cctgtgctgt atatttattc agcttgaatt gtagctcaaa tgagcaacag cgacaatcca
     7741 tgagtaattt aataaacgga tgtttttaca taattaactg aaatatattt attaatttta
     7801 taataatggt ttattaagtt tgttcaggta cgggccggga tacactgcaa catcaatgca
     7861 tgatcagcca ttattgagta tgtccttgcc gtcgattccc tcttttgtgt tgtcgggatt
     7921 actgttgctt tgcttgccgt tttcttcatt tgccagcgcc accacatcac atatcacttt
     7981 cagctacgcc gcccgccagc ggatgcaaaa tcgtgcgcgt ttattaaaac agtaccaaac
     8041 ccatctgaaa aaacaggcca gctatattgt ggaaggcaat gccgaaagca gaagggcgct
     8101 acgccagcac aaccgcgaac agataaaaca gcatccagaa tggtttcctg cgccgctcaa
     8161 ggcgagtgac agacgctggc aggcgctggc ggaaaacaac catttcttaa gcagcgacca
     8221 tctgcataac atcaccgaag tggcgattca ccgtctggag caacagcttg gcaagcctta
     8281 cgtctggggc ggtacgcggc ccgatcaagg cttcgactgt agcgggttag ttttttatgc
     8341 ctacaacaag atccttgagg cgaaactccc acgcacggct aatgagatgt atcactatcg
     8401 ccgggcaacc attgtggcga ataacgacct gcgccgggga gatttgctat ttttccatat
     8461 ccacagccgc gagattgccg accatatggg ggtgtatctg ggcgacggtc agtttattga
     8521 gtcaccgcgc accggggaaa tcattcgggt aagtcagtta gctgaacctt tctggcagga
     8581 tcacttttta ggcgcgcggc ggattttgac ggaggagacg attttgtaaa tgggtaatat
     8641 tgacgaactg gcaaaaggtc ggatgccacg cggcatccga cctgtttaca tctgggctga
     8701 aaactcacgt tctaagacat caacaatgag ctggtttttt gaaatggaat gttgtctggc
     8761 gacgatggtc agccgcgaat caatgtgatg aggaacacga agcgtcaggc gtttttgctc
     8821 ttcttcttca cgaaaagggg caataccgtc ctcttcacaa tccgccatcc actcatccag
     8881 cgcgctttcg ccttcacgat ataatgcctc aatacttgtg gctacaaagt cacaataacc
     8941 gtttacattg aggaacttgc cgcgaaatgc gttgatatcg gcttcaaatg ttactgcggc
     9001 tggttgacct ttaattgtga taatgttcga tgctttattc ataatctacc cctacacagt
     9061 taacaaacca ctccttaaga cctgctactg cgcctttatc catcacattt tttggatgtg
     9121 gctcatgtgt gctgaatttg gtcgttccaa tctgaaattt tcgtctggag ccgttgttat
     9181 tttttattgt tccacccaca gctttaatga gtgcaacaac atcatcccat ttaattccag
     9241 attttggcgg ctccgtcatt acatcgtcga atgtttttct atgacgagaa cctagcgcgc
     9301 tgaccttaat catactatca atccatgacg tcattgtatg gtgtcatcgt agtctctttg
     9361 cagtgtgacg tcaattaatg gtgtcaggtg ctgcaaagca tggaaaagaa tcccccatca
     9421 actcaacgcc cccataatct tcaactccag ctcatccggc acttcgttat acgacagcac
     9481 atgcagcccc ggcgcaaaca accttgcata acgcgccagc aaagggcgca gctgcggtgg
     9541 caccagcagc accgggtctt tccccgccgc tttcatctgc tctttcacct gtggcatcgt
     9601 actctggaac tggttgagca tattcgggtc cactggcacg ctgtcgagca tcactttacc
     9661 gccctgttgc gcctgattca ctaggttagt cagcagattt tccagctcat tattcagggt
     9721 atacaccgtc agctcctgct tgcgaacgaa cggatgggta atgctgcgcc gcagcgccag
     9781 gcgcacatcg gctgccagca gaatatgatc tttggtcacc gcgctactgg cgaccagcac
     9841 ggtggcgatg gtgacgatat cgcgcaggga aacgccttcg gtcagcagcg cccggtacac
     9901 tttcagcaac tggctgtaat tgagcaccgc gcttaaatcc tccgccaggc gcggtgccat
     9961 cgacgccaaa cggttatgca actgcgtaat atcgtcatag ttaaataaat caggaatata
    10021 gctgcgcaca atcttattta catgggtagc aatcacgctg gcgctgtcga tcacctgata
    10081 ccccatattc agcgccttcg ctttctgcgc aggctggatc caggtcaccg gcatcccgta
    10141 cgccgggtcg ttccccagca cgccgtctat ctcgccgtag gtttcgctgg agggcagcgc
    10201 catcagttta tccgccggaa tatccgcttc atcagcctta atgccgttga tgaaaatagc
    10261 gtactggctg ggcttaaggc ggaagttttc ccgaatgcgg atctccggca gcagcacgcc
    10321 gttgccgtcg gatatcacct gtcgcacgcc gcgaatacgc tgggtgagcg ggttgccctg
    10381 ggctttgtcc accagcgcca ccagcttgta gccgaggctt aagctgatgg gctcgatcag
    10441 cggaatggtt tcccaactga cctgttgctc gctggtttcg gtgatggtgc gggtcagcgt
    10501 ttcgagactt ttctcttccg cttccgccgc ctgcgggcgt ttgctcatcc gccagccggt
    10561 aaagccaagc agggcgctga acagcaaaaa cggcagatgt ggcattccag gcactaccgc
    10621 cagtacgaac ataattccgg tagcggtgta gagcaccgat gggcttgcca gcaactggtg
    10681 acgcacgtca tgggcgatat cgccgttgtc gctgacgcgg gtgacgataa tcgccgccgc
    10741 ggtggagagt agcagggaag ggatttgcgc caccagcccg tcgccgatgg tcatcagcac
    10801 atactgctgg aaggcggcgt cggcgctcag gttgtatttg aagatcccga tacagacgcc
    10861 gccgatcagg ttgatcgcca gaatcatcat cccggcgata gcatccccgc gcacaaattt
    10921 cgatgccccg tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgtcgggt
    10981 ttgtgcctgc gcctgattga tcaacccggc attaagatcg gcgtcaatcg ccatctgctt
    11041 accgggcatc gcatccaggg taaagcgggc tgaaacctcg gaaatacgct cggccccttt
    11101 ggtgacgaca ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat
    11161 cgccgcgctg cacgggcagt cgattttcag tggcggcgga ttgtcgccgt taaataaaat
    11221 cagcccgtct catccgccaa aacctgccac cgtggcggtg ccggaagaga cggagaaaaa
    11281 agcgcgagac gtcaacgaga aaacagcgct gctgaagaag aaatccgcca ccgagcttgg
    11341 cgagctggca accagtatca acaccattgc ccgtgatgcg catatggaag cgaatctgga
    11401 gatggagatt gttccccagg gattgcgcgt gctgattaaa gatgaccaga accgcaatat
    11461 gttcgaacgc ggcagcgcgc agattatgcc gttctttaaa actttgctgg tggagctcgc
    11521 gccagtgttc gactcgctcg ataacaaaat tattattacc gggcataccg atgcgatggc
    11581 ctacaaaaac aatatctaca acaactggaa cctttcgggc gatcgcgcgc tttcagctcg
    11641 tcgggtgctg gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc
    11701 ggaccagatg ctgctggatg ccaaaaatcc gcaaagcgcg ggcaatcggc gtattgagat
    11761 tatggtgctg accaaaagtg cgtccgatac gctgtatcaa tactttggtc agcatgggga
    11821 taaagtggtg cagccgctgg tgcaaaagct ggataagcag caggtgcttt cgcagcgaat
    11881 gcgctaaatg ctgaatcttt acgcatttct caaaccctga aatcactgta tactttacca
    11941 gtattgagag gtgagcaatg cgtaaaatca ttcatgtgga tatggactgc tttttcgccg
    12001 cagtggagat gcgcgacaat cccgccctgc gcgatatccc tattgctatt ggcggcagcc
    12061 gcgaacgtcg gggggtgatc agcaccgcca attatcccgc gcgtaaattt ggcgtacgta
    12121 gcgctatgcc tactgggatg gcgctcaaat tatgcccaca tctcaccttg cttccggggc
    12181 gctttgacgc ctacaaagaa gcctcaaatc atatccgtga aatcttctcg cgctacacct
    12241 cgcgcattga accgttgtca ctggatgagg cttatctcga tgtcaccgat agcgtccatt
    12301 gccacggttc tgcgaccctc atcgcccagg aaatccgcca gacgattttc aacgagctgc
    12361 acctgacggc gtctgcgggc gttgcgccgg tcaagtttct cgccaaaatc gcctccgaca
    12421 tgaataaacc caacggccag tttgtgatta ctccggcaga agttccggca tttctgcaaa
    12481 ccttaccatt ggcaaaaatc cccggcgtcg gcaaagtctc ggcggcaaaa ctggaagcga
    12541 tggggctgcg gacctgcggt gatgtgcaaa agtgtgatct ggtgattctg cttaaacgct
    12601 ttggcaaatt tggccgcatt ttgtgggagc gtagtcaggg gattgacgag cgcgacgtta
    12661 acagcgaacg gttgcgaaaa tccgtcggcg tggaacgcac gatggcggaa gatatccacc
    12721 actggtctga atgtgaagcg attatcgagc ggctgtatcc ggaacttgaa cgccgtctgg
    12781 caaaggtaaa acctgattta ctgattgccc gccagggggt gaaattaaag ttcgacgatt
    12841 ttcagcaaac cactcaggag cacgtctggc cgcggctgaa taaagctgac ttaatcgcca
    12901 ccgcgcgtaa aacctgggat gaacgccgcg gcgggcgcgg tgtgcgactg gtggggctgc
    12961 atgtgacgtt gcttgacccg caaatggaaa gacaactggt gctgggatta tgaataacat
    13021 acaaataaga gactatcagc ctggcgattt tcagcaacta tgcgctattt tcattagagc
    13081 ggttacgatg actgccagtc agcattattc accacaacaa attgccgcct gggcgcagat
    13141 tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagtcattaa
    13201 tgcaaaactg gtcggcttta ttacctgcgt tgaacactat atcgatat
//
LOCUS       DAZFVO020000134        13164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFVO020000134 DAZFVO020000000
VERSION     DAZFVO020000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.135"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(234..1256)
                     /locus_tag="ACHKNB_RS18745"
                     /old_locus_tag="pgaptmp_003749"
     CDS             complement(234..1256)
                     /locus_tag="ACHKNB_RS18745"
                     /old_locus_tag="pgaptmp_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HGU4197178.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1241..2806)
                     /locus_tag="ACHKNB_RS18750"
                     /old_locus_tag="pgaptmp_003750"
     CDS             complement(1241..2806)
                     /locus_tag="ACHKNB_RS18750"
                     /old_locus_tag="pgaptmp_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4197179.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2881..3297)
                     /locus_tag="ACHKNB_RS18755"
                     /old_locus_tag="pgaptmp_003751"
     CDS             complement(2881..3297)
                     /locus_tag="ACHKNB_RS18755"
                     /old_locus_tag="pgaptmp_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4197180.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3294..3524)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS18760"
                     /old_locus_tag="pgaptmp_003752"
     CDS             complement(3294..3524)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS18760"
                     /old_locus_tag="pgaptmp_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4197181.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3905..7699
                     /locus_tag="ACHKNB_RS18765"
                     /old_locus_tag="pgaptmp_003753"
     CDS             3905..7699
                     /locus_tag="ACHKNB_RS18765"
                     /old_locus_tag="pgaptmp_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HGU4197182.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKGMLYSIVSEYTSGKLREDYCNNLKNKIETCL
                     TELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGTGGQYDFDDVIQNENTFLS
                     LLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAIIQKKLNNDVSRLEENIKKN
                     RCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGESITDNAANIDFIYFLLSH
                     GYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETAKMPLSNIANTVAKLHGLG
                     ILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRMVRLANEIFPLWEPAAQRE
                     YIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPILSWEAVSQITREELQRLI
                     DLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSGQETLHLVVQKKLWTYSTL
                     NLQNICFSLSHESENNSDTFRKKPVALIKSLRIPNLEKYVYENISSFIRDVFIHSEEN
                     DLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENTETTEISYDQNLYSLLAHH
                     NHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDSLPLTDVQFSQLLIKAVTS
                     PHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWLAPTSTVFEQLYQALYEEG
                     DKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLILNGDKIADEVCISILNWL
                     WEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCLKNDGGSHKFIRQVLMTFG
                     HQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLTHADTRIRIEPFFNAENE
                     YD"
     gene            complement(7744..8160)
                     /locus_tag="ACHKNB_RS18770"
                     /old_locus_tag="pgaptmp_003754"
     CDS             complement(7744..8160)
                     /locus_tag="ACHKNB_RS18770"
                     /old_locus_tag="pgaptmp_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4197183.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(8157..8387)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS18775"
                     /old_locus_tag="pgaptmp_003755"
     CDS             complement(8157..8387)
                     /gene="vapB"
                     /locus_tag="ACHKNB_RS18775"
                     /old_locus_tag="pgaptmp_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HGU4197184.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            8652..9152
                     /locus_tag="ACHKNB_RS18780"
                     /old_locus_tag="pgaptmp_003756"
     CDS             8652..9152
                     /locus_tag="ACHKNB_RS18780"
                     /old_locus_tag="pgaptmp_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="HGU4197185.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLPADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQEDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
     gene            9165..9938
                     /locus_tag="ACHKNB_RS18785"
                     /old_locus_tag="pgaptmp_003757"
     CDS             9165..9938
                     /locus_tag="ACHKNB_RS18785"
                     /old_locus_tag="pgaptmp_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197186.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            10105..11238
                     /locus_tag="ACHKNB_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /pseudo
     CDS             10105..11238
                     /locus_tag="ACHKNB_RS18790"
                     /old_locus_tag="pgaptmp_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
     gene            complement(11272..11784)
                     /locus_tag="ACHKNB_RS18795"
                     /old_locus_tag="pgaptmp_003759"
     CDS             complement(11272..11784)
                     /locus_tag="ACHKNB_RS18795"
                     /old_locus_tag="pgaptmp_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197187.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            12352..12570
                     /gene="ccdA"
                     /locus_tag="ACHKNB_RS18800"
                     /old_locus_tag="pgaptmp_003760"
     CDS             12352..12570
                     /gene="ccdA"
                     /locus_tag="ACHKNB_RS18800"
                     /old_locus_tag="pgaptmp_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HGU4197188.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            12572..12877
                     /gene="ccdB"
                     /locus_tag="ACHKNB_RS18805"
                     /old_locus_tag="pgaptmp_003761"
     CDS             12572..12877
                     /gene="ccdB"
                     /locus_tag="ACHKNB_RS18805"
                     /old_locus_tag="pgaptmp_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HGU4197189.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            12878..>13164
                     /locus_tag="ACHKNB_RS18810"
                     /old_locus_tag="pgaptmp_003762"
     CDS             12878..>13164
                     /locus_tag="ACHKNB_RS18810"
                     /old_locus_tag="pgaptmp_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4197190.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSL"
BASE COUNT         3354 a         3186 c         3238 g         3386 t
ORIGIN      
        1 tcctgggggg ttaatggctg atgacgataa aatcatatat cgaatgattt atttctctgc
       61 ctgtaaatat caggccagga tttattatgc gattaatgat aaatatcata tcatctgcgg
      121 gtttccctcc acgcaggtcc ggtttccggc gaaaccggag cctcattttt cggcctgaag
      181 ttttgtcgtt gactactttt taccggcttg tgcccagttg agaagccgct gacttagttt
      241 ctgaatctcc gaagatggct ttccggaata aaatagatgt tgccccgtgc ccgtgagcag
      301 gccacgtata gcctgttacg ggatgatggt ggaagtgctg ccagcttctg ttggatgagg
      361 cgcatccagt ggttggcccc catcacgatg cagacgtcct gaaagtggtc gagtccttta
      421 ctgccccccc agttcattga gtggcatccg taacggtgat gttcgcgata gaagagcttg
      481 atcatcgtgt tgtccgcgtg cagggccgct gaacgcgctt catccgtgac gatttcaatc
      541 tgggtggtat gtgttccatg agccgaaatg cgaatgttca gttgtccggt aatgaactca
      601 cacactgtag ctgaacagcg ccatgtccgg ctgagcgtct cacaatccac catgataccc
      661 gcagccctga agcgagcctc gtatcgggtt atgtcttcat gcagcgtagc attgacgtta
      721 ccgtcgcggc ttgtgtcaaa ggtatgctga taaaaatccc cgcagcacag cactgagatt
      781 tccgcgcggc atagctccag cagaaaatta aagtcatggc cggcaaagtc ctggacttca
      841 tcaacgaaca actcatcgta gtaacgggca agtcgggtac ggatatctgg cagcaacccc
      901 cgggctgtca gcaggtgtgc gagccgccgg tgatacagcc gtccggcggg atcctgataa
      961 tgccggatat tcgtgcgcgg tattctggaa gggggctggt tgaagctcag accacgcgat
     1021 gacagttgct cctgcaggaa agggcgaaaa cagaaaccgt gcagaaactc aaaccaggtc
     1081 atcacacgga tcccgtacgg tatgtagcca aagcgtctga taatctgcgc ccgcagatgt
     1141 gcttcattat ttacggtgaa ggtcaggatc agcgtccgcc ggtcctctct taaccgacgt
     1201 atcagcagcg tggttttacc cgagccggca accgcaaata tcactctctt atccatgcca
     1261 gtgcctcctg gatataatca ggaacggtca gcttttcggc gtgcaactgt aacagctgga
     1321 atgctgcttc tgccttattg gccagcatgt aatcctgaac cgtgagtgtc cggcgggttc
     1381 cgcggaaaag cgcatcacac aggtcggcat tatcctgata caggcagatt tcaaatgtgg
     1441 agcggctgtt gtcgtggtca gcaaataccc gggagcggga acaaagcacg tcagcatagc
     1501 gttcatcgca attttgctga tagttgccgt cattgtcgcg aagggccgcg acacgatttt
     1561 ccagaagacg ggccagttca agataacgac ggaaactcgt tccgccgata gcgatgatgt
     1621 gaaccccgtc atcctccggt gccctgccat acaggcggtg ataaaaggcc tcgatcagaa
     1681 taaactcagc atccccctcc acgagcagca cgcgtctggc cagcgcaaat tccagcacgt
     1741 tattatccgg tgctttcatg aagaaggcgg cagtctcagc ggaaagttca ttcatcagta
     1801 ccggccgggt agctccgagg agaatagcct tacgtaggtc gaggcgggag gagatatggc
     1861 tactgtgtgt ggcaataaac acctgcgtct gccgttccgt ggcaagctga ttaaccagcc
     1921 tcttcatact gacatggctg agatgattct ccggctcctc aagcaacagc gcgtggatct
     1981 ctcccccctg ctgctgatgc cgctgcagcg caaactccgt tttgatgaaa cactgtcttc
     2041 cttttccgcg gtgacgaatg gaaataccgt cctcagtaat gtccaggttc gcctcaagac
     2101 ctgatttagc gccggaacgc acgccaaact gatacgtttt cagcgtgtcg tttatggcgg
     2161 acagatgccg ggcgcagaag tgcattttct gttgccggta actgttttca agccggtacc
     2221 gatcggccac cggcacattg acgctgtaaa ctgtgcgcgt atactcctgt gcggcatgct
     2281 cgttatcaat gcgtgcactg tctaagaaca gatggcgcag atagcgtctg aagcctgcga
     2341 aatgaccacc ggagaatgtg ctgaactgca cggaatagta ttcataggga aaattatcag
     2401 gatcctgctg caaaacatgg tgtatatcct gaccgtattc ctccatcatg ggagcaatgc
     2461 gcattctgag accatccgca tcgatacccg ccagattttg cctgccgttc aggtcaggct
     2521 ctcccccatt actcaggaaa acgtcagcgg tcagcacggg taactgatcg gcacggcgct
     2581 caccttcctg aaaatgcctg actgctgact gtgacaggag cgattccacc cccagcgcct
     2641 caacccgatg cctgctgtca ctcagcacaa ggtcaagagc cagcagaata gtgcttttcc
     2701 cggactcatt gtccccaacc agaatgttac gatcgttgct gaagcgcagg tccagttccg
     2761 gaaacttttt gaagttttgc agcataagcc gcgtgatcag tggcattttt ttattccttt
     2821 atataccgct gcctgaaaga gcggccggcc gcgcctgacc gcctgttttg ttttacagcg
     2881 ttatcttacc cagtcttcca gcacaagacc cggtacccgc tcaaactctc tcacattatt
     2941 cgtcaccaga atggcacagg cagcgatggc gtgtccggct atcgccgtgt cgttcggacc
     3001 gatcggcgtg ccggcgagac gcagtgccac cttaatctcc gtggtcgcat ccaccgcggc
     3061 gcggtcccac ggcaggacgg catcgagcct ctcacagaac gcgtcaacca gttgcacgtg
     3121 gcgtggcgag gccttcggac cggtggcacc gaagcgcatc tcggagtagg tgatggccga
     3181 gaccacgata cggtgaccgc gcagcaccga ctgctccagg tgcttaagca atgcttcagg
     3241 ttgctcgcgc atgatgaacg agcagatgtt cgtgtcgagc atatagatct tgttcacagg
     3301 ttaactcgtc cttcgtcgct gacaacgtcc tcgcgctccg tcataaagtc cggatcggcc
     3361 ttttctagct ccaggaatga gccccaggtc ggccggacgg ggcgcagaat gatgctgtcc
     3421 ccttcccgga cgatctccag ctcgctcacc ccctcaaaat ccagatcgcg tggaaggcgg
     3481 atagcacggt tgttgccatt tttaaaaatg gatacggttc tcatgattct ctcccgggta
     3541 agtgtggata tgtgatgcat atccggaatg atgttcattg caccgggtgt gtgcatatgt
     3601 ttagtatagg cagatgttag gcatatgtat agattcagca tatgcagatg gtgaggatat
     3661 gcgtatacca tgaatatgca gatgtcaggc atatagatat gttcagccta tgttattttc
     3721 tctctttccc cgtgcagatc cggtttctgg tgaagtcgga gcccaccatc tccgagtatt
     3781 tgataagcca attcttgtat ccctttgcct ttattatctt tttggttttc agaagttata
     3841 atgcctgtta aatcaacaat gttggaagat caacatgaga agaccatccc gaactgaagc
     3901 actgatgcca gtactgagtg ctaacaaagc tcaggaagaa cagattaaca cctcctatga
     3961 accactgact ccctcagtca ttacagatga aaaagcacaa agctatatcg aagctttgaa
     4021 ttttgcctgt tctcgcccgg atatcagaaa tattgcagta acagggccgt acggggccgg
     4081 taaaagttcg gtattactga catgggaaag ggcggaggat aatgactttc gggtgatgac
     4141 cgtatcactg gctgactttg aaatgcagcg ggcatacccc ggagattcgc tgtcagttga
     4201 gggtaagccc gactatgatg ataacgaaaa aaaagcggga aaagccgaag agaaaacaat
     4261 cgagtacagc atcctgcaac aactgctcta taaagagaaa aaaagtgtgt taccctattc
     4321 ccgtctggag cgcatttctg acgtaagtgc gtgtcagata gccatgatga cggccagtct
     4381 gttattcata ctggcttcga cggcaactgg tctgcttttt cttttcccgg attacatccg
     4441 ggaaaagctc tccctgtcgc cagaactgag tcagtttctg ctggagttgc ctgtactcgc
     4501 taggtttggt agcgccggga tatttctttt tactgcttta ttctttgcac tgaagaaatt
     4561 gcaccgtaca ggggtatttg atcggcgtgt aagtattgat aaagttgaca tactgaaagg
     4621 tgccattaca acacgccctg cggccccttc tcttctcaat gtgtacattg atgaaatcgt
     4681 ctactttttc gaacagacac aatacaatgt agtcatattt gaggatcttg accgtcataa
     4741 tgacggagcc atttttatca aactccggga aataaatcag cttatcaata actgcctccc
     4801 cacagacaat cctgtccgat ttatctacgc tgtcagggat aatcttttca taacaccaga
     4861 atcacggaca aaatttttcg atttcgtcat accagtaatc ccagtaatgg acagtgagaa
     4921 tgcttctgag cattttctaa gtaaatttac acttgatgaa ctcaggcagg aaggattcaa
     4981 agactgcctt gccagactgg ctctttttat tcctgatatg cgggttatgc ataacattgc
     5041 gaatgaattt cgtctttacc ggaatattgt gaataatgga ggagatttaa agcgtcttat
     5101 ttcactgatc acctataaaa acctttatgc tgaagactat cacaggatag atcagaaaaa
     5161 aggaatgctt tatagcattg tatctgaata tacatcaggt aaattaagag aggattattg
     5221 caataatcta aaaaacaaaa ttgaaacgtg tctaactgaa ctatctaaac tacataatga
     5281 agaagtggtc actgaacatg agttaagaag tgaaattttg cggccatata tcagtgaaaa
     5341 aacagcaccc cgattacata ttatgacagg tacaggaggg caatatgatt ttgatgatgt
     5401 aatccaaaat gaaaatacat ttctttcttt gctgagcaac caaataatca gcattaaacc
     5461 tagtggatat aacattacta taacaacaat cgataaaaaa acagctcagg ttatgaagga
     5521 ggaatatcag gaaagaatag cgattattca aaagaaacta aataatgatg tttctcgatt
     5581 ggaggaaaat attaaaaaaa acaggtgcga gattcagaat gcatcctcgt atgatcttgc
     5641 ttttttcatc aataaaatgg ggagatcagg tttcgaaaga tatattgcat gttgttcaac
     5701 gcctgaacaa catgacggag aatccattac tgataatgcc gcaaatattg attttattta
     5761 tttccttcta tctcatggtt atctttccac tgactatatg gcctaccgct cagtctttat
     5821 gccaggtagt ctgagcacgg aagacaataa ctttatcagg gctgtgacgt caggaagatt
     5881 acctgatgaa acggcaaaaa tgcccctcag caatattgct aacacagtag caaaattgca
     5941 tggactgggc atactgatgc atgacaatgc atggcatcct cagattctct ggtacctcat
     6001 gcgcaatgat acgaactctc tgaagactat catgaggatg caggcagagg tcggagccga
     6061 acggcggatg gtaagactgg ccaatgagat cttcccccta tgggaacctg ctgcgcagcg
     6121 ggaatatatc agattaatgg tcgacgggga tggtcattta tccactatga tccaccagat
     6181 tgggcgcctg aatgataccg tagctgaaca aaatcttctc ccggtattac tgtccttacc
     6241 gatactttct tgggaagccg tctctcagat aactcgggaa gaactgcaga gactcatcga
     6301 tttgcaattt aatctcgtaa cgtccttacc ggagaattgt gcgcagtttt tctgtgaaaa
     6361 cctgcggaat tcaggttgtc gcctgactaa cattcctttg gcccggagtg actctgggca
     6421 ggaaacattg catttggtgg tacagaaaaa attgtggacc tattccacgt taaacctgca
     6481 aaacatttgt ttctcacttt ctcatgagtc agagaataac agtgatacct tccgtaagaa
     6541 gcctgttgct ttaataaaat cgcttcgtat tccgaacctt gaaaaatatg tttatgaaaa
     6601 catatcatca ttcattcgtg atgtttttat acactctgaa gaaaatgatc tcattcctga
     6661 ttttcttaat tcgacattcg ttgactggga tgatgcaaag tacatgacag aaagtatgag
     6721 cttcgtactg gaagacgtat cagttatcct gaacaaagag aataccgaaa caacagaaat
     6781 ttcttatgat caaaacctgt attcacttct ggctcatcat aatcatatta ccccttgctg
     6841 gaataatgtt atttctctac tgagtgaaga tgcgtcaatt gctggtgata ctttttgtga
     6901 gtggcttaat attaattatt ctttattgcc aaatgactcg ttgcctttga ctgatgttca
     6961 gttctcgcaa ttacttatta aggctgttac atcacctcat atcagtaaag aagcgttaat
     7021 agcgataacc atggcattta ggataactct tattaatgtg ccagaaaact taccactgaa
     7081 taatgctgcg gtactaatca agcaaaaatg gctggcacca accagtactg tgtttgaaca
     7141 actttatcag gcactgtacg aagaaggtga caaactgaca tcactacttt atgcacttat
     7201 ttgtgcccgt cctgtattat tgagtgataa ttacgagctt gttttatttt ctgatgacca
     7261 gtttgacctg gggattactc gtttaattct gaatggcgat aagattgcag atgaagtctg
     7321 tatcagtatc ctgaactggc tctgggaaaa agatgaagcc ctgctctctg aggcaccact
     7381 tctcagccag caagccctga taaggttctc caccaaaata accgatgacc ggcagaagca
     7441 ggcactgctg atgcaatgtc ttaaaaatga cggtgggtca cacaagttca tcagacaggt
     7501 gctcatgacg ttcgggcatc aggattatgc cgctttcctc acagagagga attatcgtag
     7561 cattccccgt agtgatgcca tgtggcaact ggctgtccag ctgggaaata gtggatttat
     7621 caggccgccg aaacttactc acgctgatac acgtattcgt atagagccct tttttaacgc
     7681 tgaaaatgaa tacgactgac atcgcaaaat agtggtaagt ccgtcggggg cggccttact
     7741 cctttatcct gcccagtctt ccagtaccag tccaggcacc cgctcaaact ctctcacatt
     7801 attcgtcacc agtattgccc cggtggcgat ggcgtgcccg gcgatcgccg tgtcgtttgg
     7861 accgatcggc gtgccggcga ggcgcagcgc caccttaacc tccgtggttg catccaccgc
     7921 agcgcgatcc cagggcagga tggcatcgag acgggcgcag aacgcgtcaa ccagctgcac
     7981 gtggcgtggc gatgcctttg gaccggtggc gccgaagcgc atctcggagt aggtgatggc
     8041 cgagaccacg atacggtgac cgcgcagcac cgcctgctcc agattcttaa gcactgcttc
     8101 aggctgctcg cgcatgatga acgagcagat gttcgtgtcg agcatataga tcttgttcac
     8161 aggttaaatc gtccttcgtc gcagacaacg tcctcgcgtt ccgtcataaa gcccggatcg
     8221 gccttttcta gctccaggaa tgagctccag ctcgggcgga ggggacgcag aatgattctg
     8281 tccccttccc gtacaatctc cagctcactc accccctcaa aatccagatc gcggggcaga
     8341 cggatcgcgc ggttattgcc atttttgaaa atagatacgg ttctcatgtc ttcctcctgg
     8401 caaggcggct tttatatatg tttagtatat gcagatgtga tacatatgta tatgttataa
     8461 atatgcagat gtgtgagata tgcgtatgcc tgtcatatgc agatgccaaa catatggata
     8521 tgcatggtat ctgcatgttt ctctcttttt tcaccgggat ccgaaaaata tcatagtagg
     8581 aaatgccgta cctgagccgt caggctggcc ggatgatata gtggattctc gaaccggaac
     8641 gggagggtta aatgggaaat tattcagatt ttgagaccga ttttgttcag cgaacactgg
     8701 cgcttatcga tcaatataac gaaatgattg aggtgctggg caaaccattc agggagcagt
     8761 acaactacac gttgaccctg aattgcctcc tgggtcttat cgtgttaccg aaagagagag
     8821 cgctctcttt cctgcctgcc gaccgtttaa cgcggcaact taaagctgaa atgggattac
     8881 atgagtcaca gctgcccgga ccggaaatga acctgcggga gttgatccat aagatgcgga
     8941 attcagttgc tcatttctgc gtccaggtag agtcagccag cgatgcacac cttgtagact
     9001 ggattgtttt cagggaatct caggaagatg gagaagtcta cgccagcttc agcgctccgg
     9061 agcttttacc ctttctgaaa tattatgcga ccctgctgct cgacaatatg gcccgccgtc
     9121 gagcccccga tgttaacata ttggatttgt aagggattta caccatgttg attctcgatt
     9181 gctcctcacg aactcaggcg ttgcatacat tatctgccgg gtttggctgt tcccctgaaa
     9241 agctgaaaaa agtgctgctt tcgctcgatc ttgaaagtat ctacgagctt aatcctcgcc
     9301 agttggttga tgcacctcag tatctgagag aatacgtctg tgcggaactg ggcgaaccgg
     9361 gaccattcac ccgggctctg tggtttcatg gcacccggac tttcgccggt aatacttttc
     9421 cggcggggtt actggcgcta aatcagtcag aatccctcgc catgaaaatg ctgcttgacc
     9481 tggccccgaa tgagatggtc cgtacacacc tgaaggaatg ggatgttccg gggggcgttc
     9541 cggatgagat gtttcagctc agaaccggcg ataaaatcca ctgggggcct tttggccatt
     9601 tagtccggga attacatttt aacgccagtg aaaatggact gcatgattac ctctggctgc
     9661 cggagctggt agaggatgtc tgtaaggcct accagaagaa atacggccac gatcttaaac
     9721 cgcattatct gagcgtgctt catccctgta ttgtctggtt cgaggcggat attgtttatg
     9781 aaaaaggagt tcttgaaacg gctctgtcat atgcctatac atcagtccgt gacctcccgc
     9841 cggacggaaa cgcaactttc ggcatagact gtgacgggaa atctgtgtct aggtcggcca
     9901 ttgccagaat cgagtttctg cagcccgggc agatgtaatc cctaaaaaac gggtctgtga
     9961 tccggcccgt ttgttcattg ccgtgaaccc agtattgagg tcattaaagc cttaagtgga
    10021 tcacttgcag ctggctgcag aaaggtttta cattgacccg gattatgccg tttcccttcc
    10081 tcctgcctgg taaaggatct ctgaatgcac ttttatgtgg atgaaacagg ccagactggc
    10141 cgaaaccttt ttgacaaaac ctagccagtc ctctcctacg gagtgctgtc gtctgatgct
    10201 aatctggata aggttgctga agcggatcta gccgtaatac gcaaaacgct tggcgtgcag
    10261 cgcctgcacg ctgcggaact gggcttgcac cgcctttctg accttgtaga tacgctgctg
    10321 gttctgcaga aaaagcaccg gatacggttt gacatctggc aggtagtaaa gcgcgatcat
    10381 gccattattt cgttcttcga ccaggtattt gatcagggaa tgaatccagc cgtaccctgg
    10441 tctgcatact ggacccctct gcgctacccc ctgctgctga atctggccag cctgtttgat
    10501 gatgaacttg cttcaaacgc atggacggca aggctggagg cgcatgatga gcgtgcgtcg
    10561 gaactttttt gtaccgtcag tgatgaactg atcagcagga cggcagccag cgcgctggac
    10621 caccggtcga agcagctgat caccgatgcc ctgaactggg cgtcagccaa ttttgaacag
    10681 ttgggctata actgtaaaac caacaaggaa aggctgcgga ttatgcccaa tatgattggc
    10741 tttcagtccg tattgcacgg gatctgttcc cgactggggg caccagaaag aaaggccagt
    10801 atcattgttg accagcagtc acagttcaac accacccagc gtgaacttaa cgagttttac
    10861 taccagatac gggatatgcc ctgggaactg gggcccggat tacctgttat gaacatgaaa
    10921 aatatgccgg ctgaaccact tgtattccag tccggtacca agagtgcagg tctggagttg
    10981 gtagatattt atctctggac attcaagcga ttcatggaag acaaggcgct gacaaagcca
    11041 ctttcccgcc ttgtttatac caatctgaaa acagccagga ccaacagcgt gtcaatccag
    11101 tcggttgcca gtcgatttaa ggaactcctg ggtaaactcc cggtaccaag cgcagagatc
    11161 atgaggcagg cgcaggagct gcgggacttt gatgaagcca gacgcatgcc atatgtggtg
    11221 tcgggatcac ccgactgatg ccggatatta tctgctttca actgccccct cttaggggca
    11281 ggttttcagg ttaaccggca gctgtgatcc acgacgctgc ttacagtcat gctgcgatgc
    11341 cagaatgtca gcaacctgat aatcggtccc catcgccaca tcgtgcaata gcaggaaaaa
    11401 gtccccgtca ctgccgcccc tgcgcgaatc cagataccgc tgcagccatc gggcatgata
    11461 aagaacgtgg cgctgttcgc gctgcagcca tttcagcggc agacgggacg tcatatcgtc
    11521 aaggatacgc atttcctgat gaagcggcgc aataacagcg gaaaattcct ctctcagtat
    11581 ccgggcaggt ttcttctgtc cgaacagcgc ggccattttg ctcaggacat cctcgatcgc
    11641 ccggctgact ttctgggcct gatcacggga ggcctgataa cgcgtaaacc aaaacttaca
    11701 gtagaacccc gcagcggcaa gcagcgccgt aaggatcggt ccaatcagac ttgtgtccac
    11761 aatattctcc ttaaagatgg tcatgaggcc acctgaagca gccgatgacg caaatatact
    11821 ccgttttagc aattagtgct atgcgagaat gcaaaaagtg ctattcgttg tttttggtgt
    11881 tgacatttta atttatgtgt gagttttttg ttttctttca gttttctcca ctgagatccg
    11941 aaaaccccaa gttacggatc ttcctctccc tccgcacagc gttacatccc gtcagcacag
    12001 catgtagtgc ctcatacagt tgcccatggc actatatgtt gtgttgtatc tctggactgt
    12061 gatgcgccgc gcaggggcgg aaaacagcga tatgatgatt ttctcagcgt tgtacacttc
    12121 cggaaagtcg tttattcaaa taaagtcgga tccatacgaa acgggaatgc ggtaattacg
    12181 ctttgttttt ataagtcaga ttttaatttt tattggttaa cataacgaaa ggtaaaatac
    12241 ataaggctta ctaaaagcca gatagcagta tgcgtatttg cgcgctgatt tttgcggcat
    12301 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag
    12361 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tgatgtcaat
    12421 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
    12481 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
    12541 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag
    12601 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
    12661 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
    12721 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
    12781 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
    12841 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccg ttatacacag
    12901 ccagtctgca gtcatggtac cggcagtgta ttctgccggg cagcctgcat cgctgcctgt
    12961 tgccattgat tatccggcag ctctggcact tcgccagatg tcgatggtcc atgatgaact
    13021 gccaaaatat ctgctggctc cggaagtgag tgccctgctc cattacgtcc cggatctgca
    13081 ccgcaagatg ctgctggcca cactgtggaa caccggagca cgcattaatg aagcactggc
    13141 gctgacgcgg ggggattttt cgct
//
LOCUS       DAZFVO020000135        13158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFVO020000135 DAZFVO020000000
VERSION     DAZFVO020000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.136"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..183)
                     /locus_tag="ACHKNB_RS18815"
                     /old_locus_tag="pgaptmp_003763"
     CDS             complement(<1..183)
                     /locus_tag="ACHKNB_RS18815"
                     /old_locus_tag="pgaptmp_003763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197191.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQ"
     gene            120..1619
                     /gene="pitB"
                     /locus_tag="ACHKNB_RS18820"
                     /old_locus_tag="pgaptmp_003764"
     CDS             120..1619
                     /gene="pitB"
                     /locus_tag="ACHKNB_RS18820"
                     /old_locus_tag="pgaptmp_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HGU4197192.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1668..2360)
                     /gene="yghT"
                     /locus_tag="ACHKNB_RS18825"
                     /old_locus_tag="pgaptmp_003765"
     CDS             complement(1668..2360)
                     /gene="yghT"
                     /locus_tag="ACHKNB_RS18825"
                     /old_locus_tag="pgaptmp_003765"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4197193.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2534..3247
                     /gene="yghS"
                     /locus_tag="ACHKNB_RS18830"
                     /old_locus_tag="pgaptmp_003766"
     CDS             2534..3247
                     /gene="yghS"
                     /locus_tag="ACHKNB_RS18830"
                     /old_locus_tag="pgaptmp_003766"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGU4197194.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPA
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3279..4037
                     /locus_tag="ACHKNB_RS18835"
                     /old_locus_tag="pgaptmp_003767"
     CDS             3279..4037
                     /locus_tag="ACHKNB_RS18835"
                     /old_locus_tag="pgaptmp_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197195.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4083..5432
                     /locus_tag="ACHKNB_RS18840"
                     /old_locus_tag="pgaptmp_003768"
     CDS             4083..5432
                     /locus_tag="ACHKNB_RS18840"
                     /old_locus_tag="pgaptmp_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGU4197196.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5432..6256
                     /locus_tag="ACHKNB_RS18845"
                     /old_locus_tag="pgaptmp_003769"
     CDS             5432..6256
                     /locus_tag="ACHKNB_RS18845"
                     /old_locus_tag="pgaptmp_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197197.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6268..6828
                     /locus_tag="ACHKNB_RS18850"
                     /old_locus_tag="pgaptmp_003770"
     CDS             6268..6828
                     /locus_tag="ACHKNB_RS18850"
                     /old_locus_tag="pgaptmp_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HGU4197198.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6859..7929
                     /gene="lptF"
                     /locus_tag="ACHKNB_RS18855"
                     /old_locus_tag="pgaptmp_003771"
     CDS             6859..7929
                     /gene="lptF"
                     /locus_tag="ACHKNB_RS18855"
                     /old_locus_tag="pgaptmp_003771"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HGU4197199.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7926..9005
                     /gene="lptG"
                     /locus_tag="ACHKNB_RS18860"
                     /old_locus_tag="pgaptmp_003772"
     CDS             7926..9005
                     /gene="lptG"
                     /locus_tag="ACHKNB_RS18860"
                     /old_locus_tag="pgaptmp_003772"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HGU4197200.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSVPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9041..10213)
                     /locus_tag="ACHKNB_RS18865"
                     /old_locus_tag="pgaptmp_003773"
     CDS             complement(9041..10213)
                     /locus_tag="ACHKNB_RS18865"
                     /old_locus_tag="pgaptmp_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4197201.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10213..10461)
                     /locus_tag="ACHKNB_RS18870"
                     /old_locus_tag="pgaptmp_003774"
     CDS             complement(10213..10461)
                     /locus_tag="ACHKNB_RS18870"
                     /old_locus_tag="pgaptmp_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGU4197202.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10493..11407)
                     /locus_tag="ACHKNB_RS18875"
                     /old_locus_tag="pgaptmp_003775"
     CDS             complement(10493..11407)
                     /locus_tag="ACHKNB_RS18875"
                     /old_locus_tag="pgaptmp_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGU4197203.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11404..13092)
                     /locus_tag="ACHKNB_RS18880"
                     /old_locus_tag="pgaptmp_003776"
     CDS             complement(11404..13092)
                     /locus_tag="ACHKNB_RS18880"
                     /old_locus_tag="pgaptmp_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HGU4197204.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3149 a         3268 c         3416 g         3325 t
ORIGIN      
        1 ctgatacaca agaaataaca ctctttttat cgttaaaaaa tgatatttca ctttgcccat
       61 gccgttaaag tcactgataa tgcgtccgtt cgtaaattca aaatggcgta atctaatata
      121 tgctaaattt atttgttggc cttgatatat acacagggct tttgttattg cttgctctgg
      181 catttgtgtt gttctacgaa gcaatcaatg gttttcatga cacggcgaat gcggtggcaa
      241 ccgttattta tactcgtgcc atgcaaccac aacttgctgt ggtgatggcg gcatttttta
      301 acttttttgg cgtgttattg ggcggactta gcgttgccta tgccattgtc catatgttgc
      361 caaccgattt gttgctgaat atggggtcaa cccacggcct ggcgatggtc ttttccatgc
      421 tgctggcggc gattatctgg aacctgggaa cgtggttctt cggtttaccg gcctccagtt
      481 cgcacacctt gattggtgcg attatcggca tcggtttaac caacgcgctg ttaaccggct
      541 catcggtgat ggatgcgtta aacctgcgtg aagtgaccaa aattttctcc tcgctgattg
      601 tttcccctat cgtcggcctg gtcattgcgg gaggcctgat attcctgctg cgacgctact
      661 ggagcgggac gaaaaagcgt gaccgtattc accgcattcc ggaagatcgc aaaaagaaaa
      721 aaggcaaacg taaaccgcca ttctggacgc gtattgcgct gattgtttcc gctgcgggcg
      781 tggcgttttc gcacggcgcg aacgacggac aaaaagggat cggcctggtg atgctggtac
      841 tggtggggat tgcccctgct ggcttcgtcg tcaatatgaa cgcgtccggc tatgaaatta
      901 cccgtacccg cgatgccgtc accaacttcg aacactacct gcaacagcat cctgaactgc
      961 cgcagaagtt gattgcgatg gaacctccat tgcctgcagc atcgactgat ggcacgcaag
     1021 taacagagtt tcactgtcat ccggcaaata cctttgatgc gattgcgcgc gttaaaacga
     1081 tgctgccagg caatatggaa agttacgagc cgttaagcgt gagtcagcgc agccagctgc
     1141 gccgcattat gctgtgcatc tctgatacct ccgcgaagct ggcgaaactg ccaggcgtca
     1201 gtaaagaaga ccagaacctg ctgaaaaaac tgcgcagcga tatgttaagc accattgagt
     1261 acgctccggt gtggatcatc atggcggtag cactggcgct cggcattggc accatgattg
     1321 gctggcgtcg tgtagcgatg accatcggtg agaagattgg taagcgcggc atgacgtatg
     1381 cgcaaggcat ggcggcacaa atgacggcgg cagtgtctat cggtcttgcc agttatattg
     1441 ggatgcccgt ctccacaaca cacgtcctct cgtctgcagt tgcagggacg atggtggtgg
     1501 acggcggtgg gttacagcgt aaaacggtaa ccagcatcct gatggcgtgg gtatttactt
     1561 taccggcggc aatttttctt tctggtgggc tgtactggat agcattgcag ttgatttaac
     1621 cttcctgaaa atgcccggtc ctggcgatcg ggcatttcca tttttgacta gtgataacca
     1681 cgcgcggtca taaaatccgt aatcgctttt tctgcatcaa ccaacacctg ttccagtggc
     1741 tgattggcat cgatatcaac cagttgtgca ccaccaaagg ttaactgtgg cgttatggca
     1801 atcttcctcg ccagcgattc ccgtttatgg tcgggtttac gtgcacaggc aacttcaagg
     1861 tcaacattga gtttgatgac cagatcaggc ttatggctcg ccatccagtg aaacgcttta
     1921 cgttcctggc ttgccagcca tgagacaaaa cgaccacctt caacgttagg tgggaacacc
     1981 gtaccatcgt aagcgccagg aatttggtcc tgaggataac ggtcggttag aacaattaac
     2041 ccgcgacgac gacaggcaag catatgacga aagcgcagta agcgacgggc gacaaacgct
     2101 gtaattacca gcgccggaac tggtccaggc aatttttttg ctgttttcac ctgatttcgt
     2161 tcaattgttt tatgtaagga ttttcccatc aacggtaatt ttgtcactgc acgaccgaca
     2221 tttccggcct gttttcctaa atgaactctt tcggcagcac catatttttc gacaacggta
     2281 atgagatgtt cacacaccgt tgacttgcct gaaccatcgc taccaataac ggcaattaat
     2341 ggaggtgtta ttgactgcat gaatatatcc ttaaatttaa atatccatcc aaaatattta
     2401 tttggttaat atgtttttat gaaagcgtaa ttcaggtcaa tgtcacaatt aaccattgtc
     2461 acaataaggt tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac
     2521 tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt
     2581 tattgccatt gtaggatgtg atggttcagg taaatcgacc ctcacggcaa gcctggtaaa
     2641 tgaactggca gcaagaatgc cagcagaaca catttatctc gggcaatcgt ccgggcgaat
     2701 tggcgaatgg atttcacagc tccctgttat tggcgcacct tttgggcgtt atttgcgaag
     2761 taaagcggca catgtgcacg aaaagccctc aacaccgcct ggcaatatta ctgctctggt
     2821 tatctatctg ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca
     2881 gcaaggctat ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg gttttcgctt
     2941 tgatggcccg caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca
     3001 gcgcgagctg aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct
     3061 tggcattgat gaacaaacgg cgtttgcgcg taaacctgac caccaactgg cagcgttaca
     3121 ggaaaaaatc gccgttacgc cgcaactgac tttcaacggc gccaggatcc ttgaactgga
     3181 tggtcggcaa ccagccgatg aaattctgca agcgtcacta cgcgcaattc acgccgcgct
     3241 ttcctgatca ccaaaatgga taggggatga tttaacgaat ggatgcacta caaacacaaa
     3301 ctgttattag tacaaccgcg ccgcagccca gctacattcc ggggctaatt gcggtggtcg
     3361 ggtgtgatgg caccggtaaa tccacactga ccaccgacct ggtgaaatcg ctgcaacaac
     3421 actggcaaac cgagcggcgc tatctggggc tgctctccgg cgaagacggc gacaaaatca
     3481 aacgattgcc gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa
     3541 cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttct
     3601 cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg
     3661 tggtcagcga tcgcttcccg caggctgaaa tttcgggctt ttattatgac ggaccgggga
     3721 ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt
     3781 tataccaaca aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg
     3841 agactgccat ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggtg
     3901 tcatgtcgaa gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct
     3961 acagcgaagt gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc
     4021 gccgaagttt aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg
     4081 aattggcggg ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc
     4141 gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc cttgctgggg ctgttggcgc
     4201 tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat
     4261 cgtacgccaa gtcgatcagc gattttatta agtttcagac atggcaactg gtggttcagt
     4321 acggaacacc agcattaacc aacaataatc cgcagcaatt ccgcaatgtc gtctcatttt
     4381 ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt cggtggcatt gccttactgc
     4441 cattcctttc ccattcatta ggtctggatg accaaagttt ttggctggca gcgctctatt
     4501 gcacgctcat tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc
     4561 gcttcgattt aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg
     4621 tcgtagcctg gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga
     4681 atctggttgg cggcaccatg tactggtggt ttgccgcgcg cgaattacgc cgccgaaata
     4741 tccataatgc cttcaaattg aatctgtttg agtctgcccg acacatcaaa ggcgcgtgga
     4801 gttttgtctg gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca
     4861 cagtgctggt ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga
     4921 cattcttcga cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg
     4981 tgatgcgttt agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac
     5041 tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt tatcgtcggc aagccgctca
     5101 tttctctggt gtttggcgtt aagtatctcg aagcgtatga cctgattcag gtgatgctgg
     5161 gcgcaattgt gatttcgatg ctgggcttcc ctcaggagtc attgctattg atggcgggaa
     5221 aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt
     5281 tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat
     5341 gtctggacgt ggcgctctcg ttaattccaa ccttaaaggc attttttcag cgccattcct
     5401 tgctttataa cgcagccgga gagaaatcct gatgatgaga aaatatttcc cgcttgaggc
     5461 gagcgagcgt ctatttgttg cgattgaaga agatgatgtc gttgatgcac aggttagttt
     5521 gccgccgact attgcactaa gttgtaccac cgaaatcatt catgataatt acgcgttatg
     5581 cctgcaattc tggctcaatg gcgttgatcg tcaggaatta ctgcgtttag ttcgcaaaca
     5641 agccaaaggc gatgagctga ctgctgatga gcgcaaacaa tttaaataca tgcgagcgcg
     5701 ctacaagcac ctgcgttttg cgcaacggct gtatttaaag aagcatcagg ccggattttt
     5761 attcggcaaa acgaccgttt ttcttgggcg ttttcaggac ggctttcgta acggtaagaa
     5821 aaatattgtg tcgtattacg gcaacctgtt acggatttat ttaagctctc cggtctggtc
     5881 gttggtgaac tattcattac gccatagcca gttagaaagc gtaagcagtt ttattgccta
     5941 tcggcaaaag caaatgcata ccctgaaaga gattattgcc aaaccgcgac taaccggcag
     6001 ggaatttcat gatgtgcgaa aaatcatcag ccaacaggtt tcttattacg acaccttaag
     6061 atcgcttgat cctgaaaata aagaggcgtt acagatttcc cgtttcctcg ccgccattaa
     6121 tggcctgatg ggagataaac acgacgacat ggtggcggat gatatggaaa accgccagtc
     6181 gtatgatgca ccgttggcgc tggatagcga tattcgtcag cgcttagagc tgctgatttc
     6241 gcgttttccg ctataaccgg agcaatgatg tcatctcgtt tgattattgc gctgattatc
     6301 atgcttttag ccccgggtgt gcaggcacac aatttcgtca ctggcaaaac ggttacccct
     6361 gtttatattc aggaaggcgg cgagctgctt ctgaatagcg acgacgaaat tcactaccaa
     6421 aagtggaaca gcacacaatt ggcgggaaaa gtccgcatta tccagtatat cgccgggcgt
     6481 aagtcggcga agaaaaagaa ctcactgtta attaaagcgg ttgaagcggc aaactttccg
     6541 caggatcgtt ttcagcccac caccattgtg aacaccgatg atgccatctt tggtactggc
     6601 tattttgttg ttggcaaaat cgaaaaaaat aaacgccgct atccgtgggc gcaattcgtt
     6661 attgacggca atggacaggg acgcgttgcc tggcgcttgc cggagcagag ttcaaccatt
     6721 ctggtgctga ataaagccgg gcaaattcag tgggcaaagg acggcagcct gacgccggaa
     6781 gaggtagatc atgtcattgc gttggcgcag aaactgatca atgaataaaa aactcaaagg
     6841 attgagggcg ttagtgagat gaagctggtt gaacactaca tcatgcgtgg tacgcgccgt
     6901 ctggtgctga ttatcgtcgg ttttctgatc tttattttcg ccagctactc cgcacagcgt
     6961 tatctgaccg aagcggcaaa tgggacatta gctcttgatg ttgtgctgga tatcgttttc
     7021 tacaaagtac tgattgcact ggagatgttg ttacctgttg gtctgtatgt gtcagttggc
     7081 gtgacgctag ggcagatgta taccgactcg gaaattaccg ctatctctgc ggcggggggc
     7141 agtccgggac gcttgtacaa agccgttctt tatctggcga taccgctaag tatttttgtc
     7201 acccttctgt cgatgtatgg tcgtccgtgg gcttatgcgc agatttatca actggagcag
     7261 cagtcgcagt cggagctgga tgttcgccag ttgcgggcaa agaaatttaa cactaacgat
     7321 aacggacgaa tgatcctttc gcagacggtt gatcaggata ataatcgcct gactgacgcg
     7381 ctgatttata cttctactgc caatcgaacc cgtattttcc gcgcccgttc ggttgatgtg
     7441 gttgacccat cacccgagaa accgaccgtt atgttgcaca acggcactgc ctatcttctc
     7501 gatcatcagg ggcgtgacga caacgaacag atctaccgta atctgcaatt acatctgaat
     7561 ccgctggatc aaagccctaa cgtcaaacgc aaagcaaaat cggtcacgga gctggcgcgc
     7621 tccgcctttc ctgccgatca tgccgaactg caatggcgac aaagccgtgg cctgaccgca
     7681 ttgttgatgg cgctgctggc catttcatta agtcgggtaa aaccgcggca agggcgattt
     7741 tcaacgttat tgccactgac gttgctgttt attgccattt tttatggcgg cgacgtctgc
     7801 cgtacgctgg tggctaacgg cgcgattcct ctcattcctg gtttgtggtt agtgcccgga
     7861 ctcatgctaa tgggcctgct gatgctggtc gcacgcgact tctctttgct gcagaaattt
     7921 tcccgatgaa tgttttcagt cgctatttaa tccgtcatct ctttctcggt tttgccgccg
     7981 ccgcagggct attgctgccg ctttttacca ccttcaacct gattaacgaa ctggatgatg
     8041 tcagcccggg cggttatcgc tggactcagg cggtgctggt ggtgctaatg accttaccgc
     8101 gcacactggt cgaactttcg ccgtttatcg ccttattggg agggattgtt ggcctggggc
     8161 agttatcgaa aaacagtgag cttaccgcca ttcgcagcac ggggttttct atcttccgta
     8221 ttgcactggt ggcgctggtt gccgggatat tgtggactgt ttcgttaggc gcgatagatg
     8281 agtgggtggc gtcgccattg cagcaacagg cgctgcaaat caaatcgacc gccaccgcgt
     8341 tgggggagga cgatgacatt accggcaata tgctgtgggc caggcgcggc aatgaatttg
     8401 tgacggtgaa atcgctgaat gaacagggcc agcctgtggg cgtggagatt tttcattatc
     8461 gtgacgatct ttcgctcgaa tcctacattt atgcacgcag tgccaccatt aaagacgaca
     8521 aaacgtggat cctgcatggt gtgaatcata aaaaatggct taacggtaaa gaaacgctgg
     8581 aaacatcaga taatcttgcc tggcaatcgg ccttcaccag tatgaatctt gaagagttat
     8641 cagtgccggg gaatactttt tctgtccgtc agcttaatca ttacatccat tatttgcagg
     8701 aaaccgggca acccagcagc gaataccgcc ttgcactgtg ggaaaaactg gggcaaccca
     8761 tcctgaccct ggcgatgatt ttgctggctg tgccgtttac ctttactgcc ccgcgttcgc
     8821 ctgggatggg tagccgtctc gctgtaggcg tcatcgtggg cttactcacc tggatcagct
     8881 atcaaatcat ggttaatctg ggattgctat ttgcgttaag cgcacctgtt accgcgctcg
     8941 gtttaccggt agcgtttgtg ttggtggcgt tgagcctggt gtattggtat gacagacaac
     9001 attaaacccg ccgtggcgca aacaccacgg cgggctcaca ttactgcttc agagtagcaa
     9061 atgcctgaat gatggcatcg atttgctcac gactgtgggc agcatttacg ctacagcgca
     9121 gcagcgtgat gcctgcaggc gcggcaggcg gcaggataag attcacatac acgcccagcg
     9181 agataagctc gcgccagatg cgtaaacctt cctctttcga accgattatt actggcacta
     9241 ccgggctaat atgtgaactt aactcataac ccagtttttg cagaccgtca tacagatggt
     9301 tggcgttatc catcagcttt tgccgcagct ctggttgtgt ggcaatagtt cttaacgacg
     9361 agcgcacggt cgcaatgcag gacggagaag gagaggcggt aaaaatatac gggcggctgc
     9421 cgtagcgcag cacttccatc gcttccgaac cgacagcaaa accgccaatc gaggccagac
     9481 ttttactgaa ggtgccaacg ataatatcca catcatcttc aacgccgaca gcttcagcca
     9541 gtccgcgccc cgttgcgcct aacacgccaa acgaatgcgc ttcatcgaca atcaaataac
     9601 caccgagacg acgtttgata tcaacaattt ccgccagcgg cgcaacgtca ccgagcatgc
     9661 tgtagatgcc ctcaacgatg ataatcgcct ctttcgcccg ttcaccgagg cgaaccatgc
     9721 gccgttccag atctttagca tcgttatggc ggaaacgaat aatttccgcg ccaccgagag
     9781 aacaggcgtc ataaatactg gcgtggctgt cggcatccag taacaccacg gcgttatggt
     9841 cggcaagtgc gctgatgacg cctaaattag cggtataacc ggttgaaaaa acgatagcgg
     9901 tagggcgctt gaaaaactcg gcaatctctt tctctaacgc caggtgcggc gcataactgc
     9961 cgtttgccat acgagaaccg gtggtgccgg tgccttgggc cgctaacgct gcctgacctt
    10021 cggcgatggc atcatgatta aaggttaaac cgaggtaatt attggtcccg gccagaatca
    10081 cttttttatt gccgatccgg ccttcggtgg cggaatacac ttcatcaata caggtgccaa
    10141 acggtgttaa cccgttgtca ctaaattgca gtcgctcgcc agcaaggcgc gcatatttat
    10201 cgtatagccc cattatttat tctccagcca tggaagtaga gtttccatta attgcgcagg
    10261 ggttttgaca tccagcagaa tattaatagg aatggaaata tcgaatctgt cttccagcat
    10321 cattaagaga tccatcactt tgatcgactc caggccaaga tcgttgacga gatcgctgtc
    10381 gggcttaatt tccacaccgt tttcgaccaa atcctgtaaa caggaaagga tgtaatccat
    10441 tactatttca cgatttacca tagaaacgtt aacacacctt taaacaagac aatcaaaata
    10501 gcccgttgcg taatgcttgt tccaggctaa tcccgggaaa ccaattaata tcttcagaaa
    10561 tacggttatt acttgccgac cagtcggcgt gggttaattc acgaattttc gagcgggtca
    10621 gcataggttc tttacctgcc aggcgactca acgcggtact gatatccgca aggcaggtga
    10681 gtaccggcag aggaatacca accattcgca cggaaccaca acgggcgtcg gcggcaagct
    10741 gtcgtacgtg ttgccagtca tagccgccgg ggacgccatc gcataattca taggtttgcg
    10801 tctgtacagt ttcggcgctt aaccactgac ccactgcttg cgcgaaatcg gtgacgtgca
    10861 ggaaagagag ctgtgtatct ggcgcaccaa gtcgtggcag caggccgcgc agcatccagt
    10921 caaacagcgg ttttaactct ttatcgccgg gaccatacac ggctgtcggg cgaaaaacgc
    10981 ccagcgtaat ttcgtcagcc attgcagtca gacgttgttc ggcgacgtgt ttggaatttg
    11041 cgtaccagga gagctcggga tggcgcgccg ccagcgaaga gataaacaga aaacgttggc
    11101 aaaagccgct ctcttttgct gcttgcatca ggcgcaggct gccgtcaacg ttacagtggg
    11161 taaaaatctc ttctttgtgt ccgcgcactt gcccggcgca atggaccacc gcgctggctc
    11221 cggcaaccag ttcgctaagc gaatgtgtat cttccagcga accgcgcacc caagtaagat
    11281 tatcattgac gtgagcgcgg gcagtacgcg tcaatgcgcg aacgtgaaag ccgcgggcga
    11341 gcaggttatc gataatatat ttaccgataa acccggtagc gcccgtcacc gcgacagttt
    11401 gattcatgcc agggattcct gcacatgaag gcttgcagca taagccttct gataacgttt
    11461 tttcgcttcc gcacgggcag gcttgccgga ggacgttcgg ggaatgctgt ggggcggcaa
    11521 cagatcgata gccgcggtta cgccaaattc gctttggatc cgtgctgcca gcgcgtggat
    11581 aagctgtccg cgacgttctt catcgctgat ccgacactgg atctgcaaaa tgattttttc
    11641 ctgggcggta acaaaagcaa tcgcatcgcc agaatgaatt tccggttcct gttccgctat
    11701 atattcaata tcctgcggcc agatattacg gccacgaata ataatcagat ctttaatgcg
    11761 tccggtgaca tacagataac cgtccagcag ataaccgagg tcgccggtgt ctaaccagcc
    11821 cgtcgcggca atctcgtctt gcgaagcctg gtcgccaaag taaccgctca tcagactggg
    11881 gccggagatg caaatatggc ctaccacacg ttccgcgacc ggcatacctg cttcattgcg
    11941 gatttcaata ccatgttccg gcaacgcttt gccgcagttg acgaaagtcg atacggcgcg
    12001 tgtctctgca cccggcgcga cggctttacc ctgatattcg aggatgtcgc gatccacttc
    12061 gttaaccaca accccggagg cttcatcaga gaagctgaca gccagcgcat tttctgccag
    12121 tccgtagcac ggcatgaaag ttttattgtc aaagttaacc tgacggaaac attcagcgaa
    12181 ttgatggagt tgttctgcgg agatgggttc tgcaccaata ccagcgacgc gccagcagga
    12241 aagatccagt tcagcgagat ctttttcatt cacgcggcgc tggcacaatt catagccaaa
    12301 cggcggcgca acggaaacgg tgccgcgatt tttactgatc aatttaagcc attgcagagg
    12361 acgcatggca aaatcctgag tgcgcaaata atctactgaa agctgcgtgg cgacgggggt
    12421 caggagaaag ccgaccagtc ccatatcatg gtagaaaggc agccaggaga cgcagcggtc
    12481 gccagggcgt aatttaatgc cgtcgtggct tatagcacgt agattagcca tcacttcgcg
    12541 atgggtgata atgacgccac ggggaaaacg ggtgctgccg gaggtgtact ggaggtaggc
    12601 gatatcgttc ggaactggac gctggagcgc aacatcggct tccggtaatg ccttaaacca
    12661 ggcgtggctt aaaacatgta attcggggtt gttatgcgtc gcggcattga ccagtggcaa
    12721 ccactcatca ccagtgataa tggctgcggg ctggcaactt gccagtaaac cctgcaattt
    12781 ggcgctccag gaatcccgct gaccaacgcc cattggaatc gccaacggga cggcgactaa
    12841 gccggcatac tggcaggcaa aaaaagcctc tacgaacccg ctacttgttt cggcaatcag
    12901 tgccacgcga tcgccttttt tcaggttcag cgataacaac cgcttcgcac cagcttcggc
    12961 acgtgctttt aacgtctgat attccagttg atcttcaagt tggcaacgtc tgtcataaaa
    13021 attcattccg gcgctactca gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc
    13081 ggcatagcgc ataggtaggg aatgcgtaaa gattttatta gacatataca ccacgaaaaa
    13141 attggatatc caaaataa
//
LOCUS       DAZFVO020000136        13125 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFVO020000136 DAZFVO020000000
VERSION     DAZFVO020000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 13125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.137"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            282..524
                     /locus_tag="ACHKNB_RS18885"
                     /old_locus_tag="pgaptmp_003777"
     CDS             282..524
                     /locus_tag="ACHKNB_RS18885"
                     /old_locus_tag="pgaptmp_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197205.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(617..2152)
                     /locus_tag="ACHKNB_RS18890"
                     /old_locus_tag="pgaptmp_003778"
     CDS             complement(617..2152)
                     /locus_tag="ACHKNB_RS18890"
                     /old_locus_tag="pgaptmp_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HGU4197206.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSAFYAPVGMIYGKINNSFIASVMQ
                     TNLNEAGEFITGIPVLYFIVSLIIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKHNQKDSWDIVNVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGRHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAE
                     SEK"
     gene            complement(2352..2807)
                     /locus_tag="ACHKNB_RS18895"
                     /old_locus_tag="pgaptmp_003779"
     CDS             complement(2352..2807)
                     /locus_tag="ACHKNB_RS18895"
                     /old_locus_tag="pgaptmp_003779"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HGU4197207.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLDENSQVTEYFIISIATFLFLAALCLFFIYSFFKRKRVVGKLLLCIYILYISHYA
                     ITFVYCLYVVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(2895..3518)
                     /locus_tag="ACHKNB_RS18900"
                     /old_locus_tag="pgaptmp_003780"
     CDS             complement(2895..3518)
                     /locus_tag="ACHKNB_RS18900"
                     /old_locus_tag="pgaptmp_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4197208.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            3667..4040
                     /locus_tag="ACHKNB_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /pseudo
     CDS             3667..4040
                     /locus_tag="ACHKNB_RS18905"
                     /old_locus_tag="pgaptmp_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            4684..5049
                     /locus_tag="ACHKNB_RS18910"
                     /old_locus_tag="pgaptmp_003782"
     CDS             4684..5049
                     /locus_tag="ACHKNB_RS18910"
                     /old_locus_tag="pgaptmp_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197209.1"
                     /translation="MNDGRQTPPSPEAWKIRFLADGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            5059..5250
                     /locus_tag="ACHKNB_RS18915"
                     /old_locus_tag="pgaptmp_003783"
     CDS             5059..5250
                     /locus_tag="ACHKNB_RS18915"
                     /old_locus_tag="pgaptmp_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197210.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPGGE"
     gene            5284..5607
                     /locus_tag="ACHKNB_RS18920"
                     /old_locus_tag="pgaptmp_003784"
     CDS             5284..5607
                     /locus_tag="ACHKNB_RS18920"
                     /old_locus_tag="pgaptmp_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HGU4197211.1"
                     /translation="MAYHSPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMREQMSRIIRKTGTDEILFRLSKNHHLYEKK
                     KTKEG"
     gene            complement(5753..5848)
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS18925"
                     /old_locus_tag="pgaptmp_003785"
     CDS             complement(5753..5848)
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS18925"
                     /old_locus_tag="pgaptmp_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4197212.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            complement(6499..6594)
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS18930"
                     /old_locus_tag="pgaptmp_003786"
     CDS             complement(6499..6594)
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS18930"
                     /old_locus_tag="pgaptmp_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4197213.1"
                     /translation="MINLLIIVLRAVVAVVNAVIAILELIRELID"
     gene            7139..8437
                     /gene="trbA"
                     /locus_tag="ACHKNB_RS18935"
                     /old_locus_tag="pgaptmp_003787"
     CDS             7139..8437
                     /gene="trbA"
                     /locus_tag="ACHKNB_RS18935"
                     /old_locus_tag="pgaptmp_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HGU4197214.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETTETTETTETRHPADKEKAQ"
     gene            8434..9552
                     /locus_tag="ACHKNB_RS18940"
                     /old_locus_tag="pgaptmp_003788"
     CDS             8434..9552
                     /locus_tag="ACHKNB_RS18940"
                     /old_locus_tag="pgaptmp_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HGU4197215.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYSELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            9533..11836
                     /gene="trbC"
                     /locus_tag="ACHKNB_RS18945"
                     /old_locus_tag="pgaptmp_003789"
     CDS             9533..11836
                     /gene="trbC"
                     /locus_tag="ACHKNB_RS18945"
                     /old_locus_tag="pgaptmp_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HGU4197216.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKINVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            12303..12545
                     /locus_tag="ACHKNB_RS18950"
                     /old_locus_tag="pgaptmp_003790"
     CDS             12303..12545
                     /locus_tag="ACHKNB_RS18950"
                     /old_locus_tag="pgaptmp_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197217.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYFSQRP"
BASE COUNT         3689 a         3411 c         2899 g         3126 t
ORIGIN      
        1 taggcattac agtgggccct gggttgattg ataaaaaaat cgcctggggc tttctgctgc
       61 atgccgtcca caaaacgctt tcagcataca gcctcaagcg cccgccgcta ttctaatcga
      121 ttttcagtct gccacaatcg ttttccacct aatgacatgt aatgcacaac agtattttta
      181 cgggaaacta cacggcaagg attcccggaa cacgttcatt attttatggt tacagacatc
      241 atgttaaatc cggaagtaat aatgacctgg aaggaaatac tatgagtaac gaatatcaaa
      301 gcaccagtat aaacccggat gtttttgaac aattttttac cctcctgaat acacactgta
      361 aatcatcaga agctgcactt gccatgctga gtacactctg ctacgaactg atgaggagga
      421 gtgggatcca ttcactggac agcctgatgc tcaacggaga gggtatattg atacaattac
      481 agggggctga tattcgcgaa aaaacaggtc agcatattca ctgatccagg tatgggctga
      541 aaacagtgtc agcctgaaat aaacgctcaa tgcctcatac acatgaggca ttgagctcgg
      601 tcataaataa tctttattat ttttctgatt cagcatttcc ggtcagtatc ggctcttctg
      661 gcaaagacat taccggaacg tcacgttcac cattgaatac agaaacactt tcagcgaaat
      721 cattctgtaa caaagaaaaa cttaacggca gatttttggt ttgtatccct gtgatccatt
      781 gaaaaatctc gggaaaacga cgtgcactga tatgcacatg attgaggtgg tgctcagtaa
      841 tatcactggc catcatcatg agaggtacat cgtatcccga acgcaaacct ggcgcatgat
      901 gcacaggggt gctgtctgaa ctgacggatt gcccatgatc agaaaaatac agcatggcaa
      961 aatcatcacc atgctctgtc gtcatgttat aaattgcctg aagaaaccga tccagcttaa
     1021 gcaatgttga taaataacag gatactttat ttccgaataa ttccctgaac ggctgtgcat
     1081 cattaaaaag tctgtcacag ggattaggat gggaccccag catatgaatg aaaatcactt
     1141 ttggagaaga cgaattaaca gcttccctga catactgaag catatcttca tcatcaatgc
     1201 gacgggaaga agagctccct tccttaagaa aatatttctt atccgcattg gcggcgatga
     1261 cagaaatcag cgtatcatgt cgccctaaag agccctgatt tgatatccac cacgtctcat
     1321 atccggcttt tctggcaagc gtaacaatat tattacccgg attacctgtt tcagtgtaat
     1381 caagcgtcag tgttctgctg agtgaaccta tcgtattcgg agctgcggag aaatacccat
     1441 taataaatat acccggagca gaatttaacc atggtgttgt cggtaaagga tacccataaa
     1501 cagaaagata atcccgtctg acactttcac caattacgat aattatggtt ttatatttct
     1561 tctccacatt cacaatatcc catgagtctt tctgattgtg tttcagcgat tcatatcgca
     1621 tcatttcttc tctggtttca gctatccctg tcattacatt cgtaaccata cgttttggcc
     1681 atgcatttac tgacaatacg ataaagaaca acagaaagat aaatccccgg tgacttttat
     1741 cacgcttacg ccagaaccag aatgtaaaag cagcaatgat cagtgaaaca ataaaataaa
     1801 gaacaggtat gcctgtgata aattcacctg cctcatttag atttgtctgc attacagacg
     1861 caataaaact gttgtttatt tttccgtaaa tcatcccaac cggagcatag aatgcactta
     1921 acaaaagaaa gatacagaca cacgttgctg aaataacatt ctgccgcccc aacaacaaca
     1981 tcaggaacaa tataagcaca ttccccaggg tgtttttcac tgtatatccg caacaccatg
     2041 taacaagcac agaaaacaac agataaatca gtgactcttt acaccacaac ataaaataaa
     2101 actgttttgc tctctcaaaa gcatattttg atttttcttt aatacaagac acgccagtca
     2161 ttttcctgta caaacacaga cgatcatttc aacacatgga tatttatatg aaacagttca
     2221 caaaaatacc ctaccctgtc cagcagggtt tacatattat caggttctgt gtttacagca
     2281 actgctcccc gaaccagatt attcgcataa cccgatgaag gcctcataac cataaaacac
     2341 tcagaataca tttagttaac aaaaatatgt cttgccctcc cggaaaggca aacatacgaa
     2401 agagaaagca tggctataat aatatcaatc aaaatatgct caatctttcc ggttgtcata
     2461 cactcagcac cgacaacata aagacaataa acaaaagtga tagcatagtg tgatatataa
     2521 agaatataaa tgcacagaag taatttcccg acaactcttt tccgtttaaa gaaagaataa
     2581 atgaagaaca aacataaagc cgccagaaaa agaaaggtag ctatggatat tataaaatac
     2641 tccgtcacct gtgaattctc atccagagat gaatacagcg aataatatgt ggttgtgaga
     2701 ttaagtcttg ttatcaatat ataaacaact gataatagta taatataaag aatcagacca
     2761 aatctgtttt tctgcgctcc ggttaatact tctgatttat ctcgcataaa atatcctgtc
     2821 tgtaatctgt cccggtaaac aatggtaaat ttcagaatca gtgcgaatac ggtaacaaag
     2881 attcctcctg tgctttaaag tgtgtaggca gaaaccagtc tggtggttcc ggtcagggag
     2941 tctgatacct caacttcccg ggtgactata accatacctg actgacgcag tctctgcaca
     3001 tcagctgcaa ttcgctgagt tcccagccag aacaggccat ccagtgccgt taccagcaac
     3061 ccgctttcca gcgccagttt cagccgtgcc gtcggggctt taacctgccc ggcgatatca
     3121 gtaaaacaac gcgtcgtcat gccatagcag tccatccgcc gtattcgcgt cgcaagactg
     3181 tagctgaaac aatccggcac gccggataag tcattctgca gactgtatac acatccccgc
     3241 tgccgcccgt tgaacagggc ttttttctga ctgagcagcc attcccgacg cagagtgtca
     3301 atcatctgcg gtgtccgggt taacagcaga cgaaacggca gctcaaaact ggtcacataa
     3361 tcaacattca gtaatgctgt ccgcagtcgc tccctggcac ctgccccggt ctcccggcat
     3421 tcacgcatga catcagccca ctgacgcatc agtcgtggag actcattcct ggccctgaac
     3481 tgatatttct caatatgata atcaatacgt tcctgcatat cgccaccgga tatctgttgt
     3541 ttacccagac acagtgatta gcctacagga ataaatatcc tccgtgaagc caggtgcctc
     3601 agaagataaa aacatgtact tttttcaacg tgtaatatta acaccaccac cacaggaaaa
     3661 tataaaatga taaaatatat attcgcactc acacttctgc tttccgggtc tgtctgtgcc
     3721 gatcaaattc agggactcgt tattcgtgtg ctggacggag atttgtggta aatacgcccg
     3781 taaccgtgac atacacacag acagaccgct acgggcgtat tcttggacgt gtatataccg
     3841 aagacggcat ggaagttaac cgctggctgg tacagcatgg tgcagcctgg gtatatcctg
     3901 atttcaacac agattacacc ctgccggtat accagagaga agccagaaca atgaaacggg
     3961 gtctgtgggc agacaaaaaa cctgttccac catggctgtg gcgaaaagaa aataaaaata
     4021 gccacaataa ttataaataa tacatctcac agaaatatat ttcctgccgt tttatttccc
     4081 ctgccagtcc ccaacgggaa ataacctcca gaaattacac agatgtgaat aataaacctg
     4141 ttcgatgtat ccgtcacaaa agagttgatg tttgactcag aagaaaatac gcctgaagtt
     4201 aaggaaggct tcagcttcat gattgttctg acatgaacat atatccctct gtagcgccct
     4261 gcctttcata acatttctgt tacagcaaca acgaaagaca acagcaatgt cggcctttta
     4321 cgtccacatg aatattccac ttatccggaa ttgtgacaca gcccctgaat acaggattga
     4381 ttgccagtgc ccacaaatgg cacattttca gtaagattct gaatgttaca ttcccccgtc
     4441 atgtgtggca caactgtcac cgggaaagaa acggaaagca catgaaaaac atcaaaactc
     4501 acaccggact ccttatccac aaagaacaag cgcggcgcgt tcgtcttcat gaaactccgg
     4561 ccgcctggtg ccacacgcac cgggaatgct acagcaaaac gaccggtcgt cgctgcggca
     4621 gccctgacag tctcagcagg ctgattctga gcagcattca gcccatagaa tgagatgacc
     4681 cgtatgaatg atggcagaca gacgcctccg tcccctgaag catggaaaat ccggtttctg
     4741 gcggacggta cccccggcac atggttttac accgatgacg cagccgtcac aaaagagtgg
     4801 ctgcgggcac attgtgacga tgtggaagtg atccccctgt gccgtatgca tgacggtccc
     4861 gttttccggg aacaccatat taatgaactc agcgccgcac tggccagaat gctccatatg
     4921 caccacatac tgctggaacg aacccggtac agagaaaata tccatgatgc agaatgtatt
     4981 cgtgaaattc acgaagctcc cctgcaggct atcggcatcc tggaaaaata caaacgcaca
     5041 aaatcataag gcagttctat gtggatcctg attttctggc tctcatcccc ctttaatgtc
     5101 tcctccggcg atgcccggca acccacactg gccgttcaca gtcaggagtt ccgtactgaa
     5161 aaagcctgtc gggatgcctt caccgcgctg aaaaaagaaa acgtgggaga attcgctctc
     5221 cggggcgtct gcgtccccgg aggagaataa accggagatg atgattagcc acaggagtat
     5281 tcaatggcat accattcccc ggatatcaca ctgcattatc cgtatctgcg ccggatgtcc
     5341 tgggcgcaga taacggcagc agctgaagat gcggaaatac atcatgatta tgcccgggca
     5401 ttaatattgt ggcagcatgc atatcatgct gccacgctga ccctcaacaa aaacctggca
     5461 gttgctaaaa tcgacttctg tattaaacgt atccggatgc gtgaacaaat gagcaggata
     5521 atccggaaga ctggcacaga cgaaatactc ttccgtctga gtaaaaacca tcatttatat
     5581 gagaaaaaga aaacaaaaga gggatgacag tcccattgta tgggaaaaac ttgccatgct
     5641 tactgcaggt gcatttttga tactggcagt cctgggtgag taatcttaat atcctgttta
     5701 ttaaagggca gaaggcggtc cccctctgcc cttaagtacg tttccgggat tatcagtcaa
     5761 taagttcccg gatcagttcc aggagagcta tcactgcatt gaccacagca acaaccgccc
     5821 tgagaacgat gattaacagg ttaatcatcc gtttctccgt caatggagga agtcaccgtt
     5881 gcttgctcat acatcgcctg tcacaacggc tctctgacat tgttggatgt agagtacctt
     5941 cccccctgcc agagcactga acacagcacc acctgtttgt tcagccagga ctttaattca
     6001 tccatacaca gatagaacag gttttagaag caagggaggc actctgtaca gcgccaacac
     6061 aaatattata cagatgcaca cataaaattc atttaaagaa atgaatattg acaggcaatc
     6121 tgaagtcaaa ctagacaaca gatgtctggt gtcataatat tatggatgtt ggatgtagag
     6181 tatcttcctc cggtcagagc accaactccg ctccggaatc acaaaaaacc ggcctcacca
     6241 gtcggttttt ttacgtctgt acctcccctc ctccccggac aactgctctc tcactcccgt
     6301 tgaatatttc ctgttgattt ttacgaaaat acggcaaatg ggggatatcg aatatatttc
     6361 ctggtgtgta gtcggtaaaa accagctcat gtttgactca cagaaacagg ctctggaagt
     6421 acagaaagta atctgtccgt agtttattaa agggcagagg gcggttaccc actgccctta
     6481 agtacgtttc cgggattatc agtcaataag ctcccggatc agttccagga ttgctatcac
     6541 tgcattgacc acagcaacaa ctgccctgag aacgatgatt aacaggttaa tcatccgttt
     6601 ctccgtcaat ggaggaaatc actgctgcgt actcttacat tgcctgtcac aacagctctc
     6661 tcattgttag ctgaaaagag acttcctccg gccagagcac cagacacagc accacctgtt
     6721 tgtccggcca ggtctttgaa tagcacagat ccatagtccc gatggattga tgcgaaggta
     6781 agtctccaga caaggctaac ttactgatta tacttgtttc aggcgatatc gctactcccg
     6841 gaataataga ttgtaactga cagattgcga tcaggtgaat ataacgctat tctggtaacg
     6901 ttagttgaaa agagacttcc tccggtcaga gccaccaact ccaccccgga atcgcaaaaa
     6961 aaccggcctc accagtcggt ttttttgtac ctgtctccca ccaccttctg ccgttcgctc
     7021 ttctctgtga aaatgtcctg ttgaactcat tcctgtccgg ttattaccgg cgctgattcg
     7081 cattcatgcc acccgactta atcccgaaat ttatccattc atctatggag gaatccccgt
     7141 gtcttaccaa cagacgtctg ccgccgaaga tccaatggct atatggtata tcgtcggtgc
     7201 catatgcctg ctctttgcga ttatcatctg gcgcttttta cctgaaatcg tcttcgcctc
     7261 ctgcctgatc ctgcacacac tgtggggcat gattgactgg gggccctttc acaactttgc
     7321 cgccccccgt tacaacctgc tggccatcac tgctaacaac gcggcaacca tcactttcag
     7381 ccagtggctg gatgttatgt cccggacagt ggggatcctc tggttgattc tgcttcccat
     7441 gacattcggc tttctgtgga tgtggtttca ccatccggcc cagccccgtt ttacccgcag
     7501 gccactgaat atccacacac tgccccatat tttttcagct ctgtcaccgg cgatcgcccc
     7561 cgttctggct gacggagaca acaacagact cttccacgga caaaaacgtc ccgagcgccg
     7621 tgtcgcacta acccccgaag cctttgtgga gcagaacaac cttatccgca acatgcagct
     7681 cgatgttgcg tccacacgcc agtgcttcat ggcgcaactg gggcaaccac tgaccagctg
     7741 gaaagacatg gccccacatg aaaaagcgct ctttgccatc tttggcctgc agttcttcct
     7801 cggtgaccgg aaagcagcgg tagccctgat gaacaacctc aatctctcct gcaggctcaa
     7861 aagtaaacgg gaccaggggc gtttcagcac gccggtgtat tcactggcca gaaacgcctt
     7921 tatccgggtc atcaaaacag aaggcgcaca aaagtggctc aggcagcacc gttacgttcg
     7981 cagcggtctg gtctggcttt acgcacatga tttgcgcctg acgcctccga actggctgtg
     8041 gctcaagggc gttgaccgga cgctcttcta tgcccttcac cgggcaaaca ccacaaaagg
     8101 cttcatcgaa ggggccggcg tggtagccgt tgcccgggca gaaaatgaag cgtgtcgtct
     8161 cggcctgccc tgcccggaac cctgtgtgga agaagccatt gaagggctgc ggcaggacat
     8221 gctgagactg gggttgatat gggacgaacc tcagccagac cgcgatcgca gacgtcagat
     8281 aaggacccgc tggtcactca cggatgacgt gataccacgc cgccatgata acgatgaagg
     8341 ctcagatact ggtgaaacca ccgaaaccac tgaaaccact gaaaccactg aaaccactga
     8401 aacccggcat cccgccgaca aggaaaaagc acaatgacca tccgctacac tgcaacactg
     8461 aacaataaac acctgtccct gaccgccctg taccctgacg gtggtcagga ggttattgcc
     8521 atcctgccgg ttacagaact gggcgcactg attaaccgta ataacaggct gatgcacctg
     8581 tccttcagca ccccttttcg tgaaataacc gaaacactgc ttgttgatac cgttgatact
     8641 gaacgtcccg gacatcacat cggcagaatg ctctccgcga ttgacagctg tctgagcgcg
     8701 gcatattcag aacttcctgc ccggaaaacc agcttcatga gagggctgat atacggtgca
     8761 ggattagtgc tggcacttct gacagcggta acagccggat atattttact gaaaccggac
     8821 agcgattccc ctctcagggt acctgtcaca gaatccgtaa cagaaacagc accattacac
     8881 atcgacaagg aaattgtccg gtcagtagcc ccgtcagaga cgaaaaatga aatcgttcac
     8941 ccggcaccac aggaaatgca ggagacatcc gctaccccgg cgataacagc agacaaaaat
     9001 actctggtcg ctgaagcccg ccgtaatctg gccactgtac ttaaacgcaa cgcggaccgg
     9061 ggtatgttca ccattaatct ctccactggt catgaacgca cactctacgc cttccttgac
     9121 cccgcatgca tcaactgccg tcttctggag cccgcgctta agcagctggc ccgggaattc
     9181 agcgtggtga tttatccggt ctccgtaatc ggcggagagg ccagcacaaa ccagactgcg
     9241 ccactgctgt gtgaaccgga cgcacaaaaa cgggcgaccg gatggcatca gctgtattcc
     9301 gcagacggtg gtatggcacc caccggcgat gcaccggctg caccggatga aaagtgtctg
     9361 accgctgctc gtgctgccat cgatgtcaat aaccaggctt tccgccagtt cggttttggc
     9421 ggtaccccat gggtgttatc cgataccggc tggcacctgc cgtccggcat cctgaaagac
     9481 gccactaccg tcagcctgtt cctgaaagcc acagataccg aggataatca caatgagcca
     9541 gcatcacgtt aatcctgacc tcatacaccg cacggcctgg ggaaacccgc tgtggaatgc
     9601 actgcacaac ctgaacatca ttggtctgtg tctggccggc agtatcatta cggccctgat
     9661 atggccactg gcccttcctg tctgtctgct gttcaccctg gtcacctccg tgatattcac
     9721 gctgcaacgc tggcgatgcc ccctgcgtat gcccatgacg ctcagccttg atgacccctc
     9781 ccaggacaga aaggtcaggc gaagcctctt cagcttctgg cccaccctgt ttcagtatga
     9841 agcagatgaa acatcccccg cacgggggat tttttatgtc ggttaccgcc gtatcaacga
     9901 tatcggtcgc gaactctggc tgagcatgga tgacctcacc cggcatatca tatttttttc
     9961 caccaccggt ggcggtaaaa cagaaaccac ctttgcctgg ctgctcaacc ctctctgctg
    10021 gggaagaggc tttacctttg ttgacggaaa ggcacagaac gataccacga ggacaatctg
    10081 gtatctctcc cgacgttttg gccgggagga tgatatcgag gtcatcaact tcatgaacgg
    10141 cggaaaatcc cgcagtgaaa tcattcagag cggcgaaaaa tcccgcccac agtcaaacac
    10201 ctggaacccg ttcgcattca gcaccgaagc cttcaccgcc gaaaccatgc agtccatgct
    10261 accccagaac gttcaggggg gagaatggca gtcacgggcg atagccatga acaaagccct
    10321 ggtctttggc acaaaattct ggtgcgtccg ggaaagaaaa accatgtccc tgcagatgtt
    10381 acgggagcat atgacgctgg aagggatggc aaaactctat tgccgtgggc ttgatgacca
    10441 gtggccggaa gaagccatcg caccactgag aaattacctg caggacgttc cgggattcga
    10501 tatgtcactg gtgagaacgc cgtcagcctg gactgaggag ccccgtaaac agcatgctta
    10561 tctctccggt cagttctcgg aaacattcac aacctttgcc gaaaccttcg gggacgtctt
    10621 tgcggcagat gccggcgaca ttgatattcg cgacagtatc cacagcgacc gaattctgat
    10681 tgtgatgatc cccgcactgg acacatcagc ccataccacg tcggcactcg gtcgtatgtt
    10741 catcacccag cagagcatga tcctcgcccg tgacctgggc taccgcctgg aaggcacaga
    10801 cgcacagacc ctggaagtca aaaaatacaa aggccgcttc ccctatatct gtatccttga
    10861 cgaagtcggt gcctactaca cggaccgcat tgcagtggag gccacccagg tacgttcact
    10921 ggagttctcc ctgataatga cggcgcagga tcaggagcga atcgaagggc agacttctgc
    10981 caccaacacc gccacgctga tgcagaacgc cggcaccaaa ttcgccggac gcattgtcag
    11041 tgacgacaaa acagcaagga cggtgaaaaa tgccgccggt gaagaggccc gcgccagaat
    11101 gggcagcctg cagcgccacg atggtgtgat gggtgagtcc tgggtggacg gtaaccagat
    11161 cacaatccag atggaaagca aaatcaacgt gcaggatttg atcagactga atgccggtga
    11221 gttctttacc gttttccagg gggatgtggt gccgtcagcc tctgtgtata ttccggacag
    11281 cgagaaaagt tgcgacagtg atccagtggt catcaaccgt tatatctccg ttgaagcccc
    11341 ccgactggag cgtctccgcc gtcttgtccc caggacagtg cagcgcaggc tccccacacc
    11401 ggagcatgtc agcagcatta tcggtgtgct gacagaaaaa acctcccgga aacgtcgtaa
    11461 aaaacacaca gagccttacc gaatcatcga taccttccag gaccagctta aaagaacaca
    11521 aacatcgctg gatctgcttc cgcaatatga tacagatatc gaatcccggg caaacgaact
    11581 gtggaaaaag gctgtgcaca ctatcaataa cacaaccagg gaagaacgcc gggtatgtta
    11641 tatcacactg aatcgtccgg aagaggaaca ctctggtcct gaagatatac cttcagtgaa
    11701 ggcgattctg aatactctgc tccccctgga gatgctgtta cctgtaccgg atctaactgc
    11761 atccccgccc cataaaaaaa atgtcgctca gacaccatca ggtggaaaac aagagagtcg
    11821 taaaaaaaga ttctgataac acctgtatgg ctgattttaa taacaacctt tacgcgatta
    11881 ttaatctcct ccgctcacca tagtaagcgg aggattcagg atttcaatcc atcaccagag
    11941 gtacaataaa tgtgtattag tagcccggaa acaaactcat ggtctgttat ttatcgtaaa
    12001 aattccggag aagatatcaa tatcacttcc ctgacgttta aaaatagtct tctggcagcc
    12061 cggacactga tggtgcccga aaactatatt atatgcatac tcagaaacgg ggagagggtc
    12121 agacgatggg acagagaaat acttgcaggc tcaaacagat ggtacaagtg ttctcccgat
    12181 aatttcgaaa tactgggtaa actccccata ataaataaag tcacaacact gataaaatca
    12241 taatctacgg attttaatca gttaagctaa atcaggatga acgctttgat tatgaattat
    12301 acatgaaaac aaatatggga agcacaaaaa atggtgccag ggttccccac gttcgtaata
    12361 gattcacttc tatggatatt ggtatgaatt cccagatcct gccagacatg ctgattaatt
    12421 ttgcccttat taacatcaca gacagaaaga acgagggtac caataccatt gatggtaact
    12481 ggcaggccga tgaaggacgc cgttaccggg ataatgtaag aatctatttc agccaacgcc
    12541 cttagctcgt tttctgaaaa agtcgtcctg aagtcatgta tctgcttcca ttctcatgtt
    12601 catatttctc cttacgcttt cactgtctgt catcggaagt accgatttat tttagtgctg
    12661 ctcacttaca gccattcacg gaagaggatt gtggtgaaca gtaggagtct ggctgctggc
    12721 gagggaaggt tcaggactgg tatggggttt agtgctggag gatgactttg aatcccggac
    12781 tggtcagtga ggtgatgatc tggtgtaact caatctgcca gggtaaactc tccttgcttt
    12841 tgcgcacaag cagtgggccc tggttcgctt atgtcctgca tctacaagcg aatgactgac
    12901 cggagactcc catatctggt cagtcgccca tttttatttt ctaccaaatc agacaagcat
    12961 accgtaataa ataacgccag tcccctgtca ctctcgtgtg ctctactgga atgttgttga
    13021 tggagatatc cagatgaaag agcttacatc ttcgacagga ctataaatta tgcagtaaag
    13081 agttccccgg agaaaggggc tgttctgctg ttctcatcaa gtcgg
//
LOCUS       DAZFVO020000137        12993 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFVO020000137 DAZFVO020000000
VERSION     DAZFVO020000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.138"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            302..883
                     /gene="mdaB"
                     /locus_tag="ACHKNB_RS18955"
                     /old_locus_tag="pgaptmp_003791"
     CDS             302..883
                     /gene="mdaB"
                     /locus_tag="ACHKNB_RS18955"
                     /old_locus_tag="pgaptmp_003791"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HGU4197218.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            914..1228
                     /locus_tag="ACHKNB_RS18960"
                     /old_locus_tag="pgaptmp_003792"
     CDS             914..1228
                     /locus_tag="ACHKNB_RS18960"
                     /old_locus_tag="pgaptmp_003792"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HGU4197219.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1276..3168)
                     /gene="parE"
                     /locus_tag="ACHKNB_RS18965"
                     /old_locus_tag="pgaptmp_003793"
     CDS             complement(1276..3168)
                     /gene="parE"
                     /locus_tag="ACHKNB_RS18965"
                     /old_locus_tag="pgaptmp_003793"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGU4197220.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQHRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3197..3778)
                     /gene="yqiA"
                     /locus_tag="ACHKNB_RS18970"
                     /old_locus_tag="pgaptmp_003794"
     CDS             complement(3197..3778)
                     /gene="yqiA"
                     /locus_tag="ACHKNB_RS18970"
                     /old_locus_tag="pgaptmp_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HGU4197221.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3778..4605)
                     /gene="cpdA"
                     /locus_tag="ACHKNB_RS18975"
                     /old_locus_tag="pgaptmp_003795"
     CDS             complement(3778..4605)
                     /gene="cpdA"
                     /locus_tag="ACHKNB_RS18975"
                     /old_locus_tag="pgaptmp_003795"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HGU4197222.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPYQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4630..5052)
                     /locus_tag="ACHKNB_RS18980"
                     /old_locus_tag="pgaptmp_003796"
     CDS             complement(4630..5052)
                     /locus_tag="ACHKNB_RS18980"
                     /old_locus_tag="pgaptmp_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HGU4197223.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5053..5682)
                     /gene="nudF"
                     /locus_tag="ACHKNB_RS18985"
                     /old_locus_tag="pgaptmp_003797"
     CDS             complement(5053..5682)
                     /gene="nudF"
                     /locus_tag="ACHKNB_RS18985"
                     /old_locus_tag="pgaptmp_003797"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HGU4197224.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5887..7368
                     /gene="tolC"
                     /locus_tag="ACHKNB_RS18990"
                     /old_locus_tag="pgaptmp_003798"
     CDS             5887..7368
                     /gene="tolC"
                     /locus_tag="ACHKNB_RS18990"
                     /old_locus_tag="pgaptmp_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HGU4197225.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7516..8187
                     /locus_tag="ACHKNB_RS18995"
                     /old_locus_tag="pgaptmp_003799"
     CDS             7516..8187
                     /locus_tag="ACHKNB_RS18995"
                     /old_locus_tag="pgaptmp_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HGU4197226.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8193..9353
                     /locus_tag="ACHKNB_RS19000"
                     /old_locus_tag="pgaptmp_003800"
     CDS             8193..9353
                     /locus_tag="ACHKNB_RS19000"
                     /old_locus_tag="pgaptmp_003800"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HGU4197227.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(9391..10179)
                     /gene="ygiD"
                     /locus_tag="ACHKNB_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /pseudo
     CDS             complement(9391..10179)
                     /gene="ygiD"
                     /locus_tag="ACHKNB_RS19005"
                     /old_locus_tag="pgaptmp_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
     gene            10322..11095
                     /gene="zupT"
                     /locus_tag="ACHKNB_RS19010"
                     /old_locus_tag="pgaptmp_003802"
     CDS             10322..11095
                     /gene="zupT"
                     /locus_tag="ACHKNB_RS19010"
                     /old_locus_tag="pgaptmp_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HGU4197228.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAAMGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11153..11323)
                     /gene="yqiD"
                     /locus_tag="ACHKNB_RS19015"
                     /old_locus_tag="pgaptmp_003803"
     CDS             complement(11153..11323)
                     /gene="yqiD"
                     /locus_tag="ACHKNB_RS19015"
                     /old_locus_tag="pgaptmp_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HGU4197229.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11584..12237)
                     /gene="ribB"
                     /locus_tag="ACHKNB_RS19020"
                     /old_locus_tag="pgaptmp_003804"
     CDS             complement(11584..12237)
                     /gene="ribB"
                     /locus_tag="ACHKNB_RS19020"
                     /old_locus_tag="pgaptmp_003804"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HGU4197230.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12341..12489)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12611..12901
                     /gene="ubiK"
                     /locus_tag="ACHKNB_RS19025"
                     /old_locus_tag="pgaptmp_003805"
     CDS             12611..12901
                     /gene="ubiK"
                     /locus_tag="ACHKNB_RS19025"
                     /old_locus_tag="pgaptmp_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HGU4197231.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3292 a         3465 c         3160 g         3076 t
ORIGIN      
        1 aaaatccatt tataattatc aacatgacac gttgatattt tcatctttta cttattcaaa
       61 gccatatttt caatcccaaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta
      121 agaattatga ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg
      181 tgagtcagag caagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
      241 tttcaccgta ccgctctgtg acgtattata gtcggcaaac gtctcacctt gaggttaaaa
      301 aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact
      361 gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg
      421 catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc
      481 tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa
      541 atatattgat gatgtattca ccgaaggtca tggaacgctg tatgccagcg atggccgtac
      601 ccgcaaagat ccgtcaaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat
      661 gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc aattcttcca
      721 cggcgttggc gtcgacggtg tgtatctgcc gttccataaa gcaaaccaat tccttggcat
      781 ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta
      841 tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta
      901 gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca
      961 ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg
     1021 ttgccacggc tatgcgccaa tggtggattg cgctgctggt gtgagtttcc agtctatggc
     1081 accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct
     1141 gcaaaccccg cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa
     1201 tatccgcatc ctgcaacctg ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg
     1261 gcaatgttct ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt
     1321 gcggcgatct tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg
     1381 atcatcttca tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt
     1441 ttcacgcaat tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt
     1501 gcctttcttg cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta
     1561 ataaacctct ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc
     1621 gtgtttcacc aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg
     1681 cagaccatca gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag
     1741 atcgtcgctg tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc
     1801 cagcacttcg tcggaagaaa cttcccaggt gttaaggatc ttacctttca gtggcatgat
     1861 cgcctgatat tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac
     1921 aaggaacagt tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag
     1981 tgctgggccg ctggtcagct ttttgcgcac cactttttta gccgcacgca tacggtgctg
     2041 ggcgctggaa atcgccatct ccgccagcag ctccgccgcc tgaacgttct ggttcagcca
     2101 caggataaag gcatctttca ccacgcctga aacgaatgcc gcgcactgac gcgaagagag
     2161 acgctctttg gtctgcccgg caaactgcgg atcctgcatt tttactgaca gcacataggc
     2221 gcagcgatcc cagatatctt ccgccgacaa ctttacaccg cgcggcagaa tgttgcggta
     2281 ttcacagaac tcacgcatcg cgtccaacag gccctgacgc agaccgttaa cgtgcgtacc
     2341 gccctgcatc gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg
     2401 cagccacagt agcgcccagt ccacagcttc agtatcacca gcgaaattac cgataaacgg
     2461 tttttctggc agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc
     2521 ctggtagcac cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc
     2581 agggcacagt actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg
     2641 gctgtcaaag aaggtttcat ccggccagaa gtgcacgctg gtaccagtat tgcgtttacc
     2701 gcaagtgccg acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata
     2761 aacctgacca tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga
     2821 aatccccacg ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc
     2881 tgcatgcaga cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc
     2941 caccggcatc ccgcgcccat cgtcaataac ttctaacgac tggtcagcat gtaagattac
     3001 atccacgcgt tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg
     3061 ccccaaatgg ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc
     3121 aagcccggtg agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag
     3181 caattcgagt tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat
     3241 aatcttcgaa gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag
     3301 cgtagtacgc caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc
     3361 agatcaaatc cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc
     3421 gtgactctag cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg
     3481 tcagcagttc aaacgggcgc accgccgggt ttaccaccac tgcgggcagc ataaaacatt
     3541 gtgacaacca ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc
     3601 cgccatgttc caggacaatg gattccagca gctctgccgc gtcggaagga tacggcggca
     3661 actgcggaat gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc
     3721 tcgcttttgc agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca
     3781 gtagccttct gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt
     3841 ggtcagcgtg ccatcagcat gtaactcgag agtacgccag ccgggcgcga tagtatccag
     3901 cgtaaagttg gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg
     3961 gcgaccgttc cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac
     4021 gtgcggaaac ttcgccagca cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc
     4081 gagccaacta caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc
     4141 tggcgcatcg gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg
     4201 cggcacgcca aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac
     4261 gcgcttcgcc ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc
     4321 gtggttgccc ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg
     4381 ctgataggcc gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa
     4441 ttcgtgctga tatggacgaa tcgcctccag caccgcctgg tagctttccc aggtgtttac
     4501 ccctaacagg gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct
     4561 gactctggcc tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt
     4621 ttcacgacgc tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca
     4681 gtccgctaaa aactggttaa tttgatgctt ttcgtcgcgt tgatgcaatt ttttattagg
     4741 ataatcatac cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat
     4801 cgcgtcatga tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc
     4861 agtctgttct attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata
     4921 ttgtgcgttt gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa
     4981 acggcgcaat tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt
     5041 gtaacgcttc atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg
     5101 caaagcgatg accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg
     5161 ttcccggctt accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc
     5221 gcttgcggtc gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc
     5281 cgggcttgcc aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc
     5341 aatcgcttcg cgacgggcaa catcttccac actttcaccc tcttctatca tcccggcaac
     5401 catctccagt agccaggggg tttcactggt gtcgtatgcg gcaatccgaa tctgctcaat
     5461 cagcacaact tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc
     5521 aaaaatttcc ctccgtacct catgactcat ttgcccgttg aataaacgat gacgaaatct
     5581 ataaagatct aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc
     5641 gtttttgcca aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact
     5701 aatagtgaat taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt
     5761 aaattgaggc acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggcttc
     5821 tgctagaatc cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga
     5881 atgcaaatga agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg
     5941 ttgagccagg ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa
     6001 ttgcgtaagt ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt
     6061 ccattactgc cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac
     6121 gcgaacggca tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt
     6181 gatatgtcga aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc
     6241 acatatcaga ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg
     6301 ttgaatgcta ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa
     6361 ttagatcaaa ccacccaacg ttttaacgtg ggcctggtag cgataaccga cgtgcagaac
     6421 gcccgcgcgc agtacgatac cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat
     6481 aacgcggtag agcagctgcg ccagatcacc ggtaactact atccagaact ggcagcgctg
     6541 aatgtcgaaa actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc
     6601 gaaaaacgca acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa
     6661 attcgccagg cgcaggatgg tcacttaccg acgctggatt taacggcttc taccgggatt
     6721 tctgacacct cttatagcgg ttcgaaaact cgtggtgccg ctggtaccca gtatgacgac
     6781 agcaatatgg gccagaacaa agtgggcctg agcttctcgc tgccgattta tcagggcgga
     6841 atggttaact cgcaggtgaa acaggcccag tacaactttg ttggtgccag cgagcaactg
     6901 gaaagcgcgc atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgca
     6961 tctatcagta gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac
     7021 gcgatggaag cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcaacc
     7081 accacgctgt acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaat
     7141 cagctgaata ttaagtcagc cctgggtacg ttgaacgagc aggatctgct ggcactgaac
     7201 aatgcgctga gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa
     7261 cagaatgcta ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca
     7321 tccgcacgca ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg
     7381 ggcttcggcc ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt
     7441 cccccttctc gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg
     7501 tcccggaaga caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac
     7561 tggagcgcgc gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct
     7621 ggctgtgaaa agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct
     7681 gcaaacccag gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc
     7741 gaacgtactg cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt
     7801 cagtgccagc aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag
     7861 caatccagcg ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg
     7921 ggcggcggcg cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagcccg
     7981 gcttacggta aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc
     8041 accatgaccg taccgaagac agccatggca ccaaaaccgg cgaccaccac taccgttacc
     8101 cgtggcggtt ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaactggc
     8161 acctcttctc gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc
     8221 cggactggcg tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc
     8281 cgtactggtg tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag
     8341 aagtcaccgc cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg
     8401 atgagctgat gaccaaattc cgcattccaa aacacacctg gagttttgtg cgtcagtcat
     8461 ggctgactca ccagccatcg ctttattcgc gtcttgatct ggcatgggat ggcactggcg
     8521 aacctaaact gctggaaaat aacgccgata caccaacgtc actatacgag gcggcgttct
     8581 ttcagtggat ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc
     8641 agtttaacag tctgcaagaa aagctaatcg atcgctttgt cgagttgcgt gaacagtatg
     8701 gcttccagtt gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc
     8761 agtatttgca ggactgcgcg acggaagctg aaatcgctac cgagttcctc tacattgacg
     8821 atatcgggtt aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc
     8881 tgttcaagct gtatccatgg gaatttatgt tgcgtgagat gttctccacc aagctggagg
     8941 atgcaggcgt acgctggctg gaaccggcgt ggaagagcat tatctccaac aaagcgcttc
     9001 taccgctact gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg
     9061 aagatgatca tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg
     9121 caaacgtgtc gatcattgag aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg
     9181 aagaagggat gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc
     9241 tgattggtag ctggctggtg aacgatcaac ctgccggaat tggcattcgt gaagaccgtg
     9301 cgttgatcac ccaggatatg tctcggtttt atccacatat ttttattgag taatcaatga
     9361 tgccggatgg cattcgccat ccggtaattg ttaacctatc tgcaccgaca gcatactcag
     9421 gctgcccatt tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc
     9481 taagacatac aacaacggca gatagtgctc cggcgttggg ttcgataacg cgccaccttc
     9541 atggtcgagg taattcacca gaggatgctg ctctaccgcc ccttgccacg tcagattcgc
     9601 tttcacatac tcattaaacg acatcgccca aggatatggt gaactatcac cgtgccactt
     9661 cactgtacgc aggttatgca ccacgttgcc actggcgacc aacattattc cttcatctcg
     9721 cagcgctgcc agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat
     9781 actcaactgc accatcggga tatcggcgtc aggatacatc ttaatcagca cgccctagga
     9841 accgtggtca aagccccagg cttctttatc cagcgtcacc gggaccggcg ctaacagctc
     9901 aaccagatgc tgtgccagca taggcgaacc cggagcagga taatgcgtat cgtacagcgc
     9961 ctgcgggaag ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt
    10021 tccacgggta aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat
    10081 ccccaacttc tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc
    10141 gtgacctaaa aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg
    10201 tgtcattttg atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg
    10261 atgatgatca tcagttattt tgacgatctg cctgaaagtg aagatttata aggagttgtc
    10321 gatgtcagta cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt
    10381 tctcggcgtt ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc
    10441 ggcggggatc atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga
    10501 aggaatgtcg cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg
    10561 cctggaccgc atgttgccac atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc
    10621 gttgccaaaa tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa
    10681 cttcccggaa gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg
    10741 catcgcactg gccgtcgctt tgcacaatat ccctgaaggt ctggcagtgg caggcccggt
    10801 ttatgcggca atggggtcta aacgcaccgc gattctgtgg gcggggattt ctggactagc
    10861 agaaattctt ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt ccccggtggt
    10921 catggcggca atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt
    10981 aatgccgctc gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg
    11041 aatgtcagtg atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaaaca
    11101 acatcagaag ttgtccagca catggacaac ttctgccagc ttcacgaaag ccttattcta
    11161 ccttttcggc tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg
    11221 cgtctctgtc ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca
    11281 caaccagagc aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat
    11341 ttaacaatac taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata
    11401 tggtcgcata ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat
    11461 attaatgaaa ttttctcata gaaagagaag cggaattaac atcttgcata gcggggcaaa
    11521 ataaaaaccg aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg
    11581 ttttcagctg gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac
    11641 gagcgccata ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc
    11701 atcgtcatta gtcagctcac acagcacacc agccggttta aagcctgcca gcgtcatcaa
    11761 atcaatagtc gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag
    11821 tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc
    11881 gcgaacggtc gtaatacgat cagcggcaga aacaccggta gtcacacctt cagccgcttc
    11941 aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag
    12001 atcaagttgt ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg
    12061 aatggtcagc gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc
    12121 gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag
    12181 tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt
    12241 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
    12301 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
    12361 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
    12421 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
    12481 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
    12541 attctggttt atgacttacc cttatcgcac tacaatggta ctcaacacct atcagtacag
    12601 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
    12661 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
    12721 acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca
    12781 agtcctgtta cggactcgtg agaaactggc gctgcttgaa caaagaatga gcgaactgga
    12841 aaatcgcagt actgagatta aaaagcagcc agatccagag actctccccc caacgctgta
    12901 aacatttgtc tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgctttct
    12961 attaccttat cgcttaattc cattcagaat atc
//
LOCUS       DAZFVO020000138        12961 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFVO020000138 DAZFVO020000000
VERSION     DAZFVO020000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.139"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            148..1257
                     /gene="livK"
                     /locus_tag="ACHKNB_RS19030"
                     /old_locus_tag="pgaptmp_003806"
     CDS             148..1257
                     /gene="livK"
                     /locus_tag="ACHKNB_RS19030"
                     /old_locus_tag="pgaptmp_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HGU4197232.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1305..2231
                     /gene="livH"
                     /locus_tag="ACHKNB_RS19035"
                     /old_locus_tag="pgaptmp_003807"
     CDS             1305..2231
                     /gene="livH"
                     /locus_tag="ACHKNB_RS19035"
                     /old_locus_tag="pgaptmp_003807"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HGU4197233.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALIMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2228..3505
                     /gene="livM"
                     /locus_tag="ACHKNB_RS19040"
                     /old_locus_tag="pgaptmp_003808"
     CDS             2228..3505
                     /gene="livM"
                     /locus_tag="ACHKNB_RS19040"
                     /old_locus_tag="pgaptmp_003808"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HGU4197234.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3502..4269
                     /gene="livG"
                     /locus_tag="ACHKNB_RS19045"
                     /old_locus_tag="pgaptmp_003809"
     CDS             3502..4269
                     /gene="livG"
                     /locus_tag="ACHKNB_RS19045"
                     /old_locus_tag="pgaptmp_003809"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HGU4197235.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4271..4987
                     /gene="livF"
                     /locus_tag="ACHKNB_RS19050"
                     /old_locus_tag="pgaptmp_003810"
     CDS             4271..4987
                     /gene="livF"
                     /locus_tag="ACHKNB_RS19050"
                     /old_locus_tag="pgaptmp_003810"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HGU4197236.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGGQ"
     gene            5380..6696
                     /gene="ugpB"
                     /locus_tag="ACHKNB_RS19055"
                     /old_locus_tag="pgaptmp_003811"
     CDS             5380..6696
                     /gene="ugpB"
                     /locus_tag="ACHKNB_RS19055"
                     /old_locus_tag="pgaptmp_003811"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HGU4197237.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6794..7681
                     /gene="ugpA"
                     /locus_tag="ACHKNB_RS19060"
                     /old_locus_tag="pgaptmp_003812"
     CDS             6794..7681
                     /gene="ugpA"
                     /locus_tag="ACHKNB_RS19060"
                     /old_locus_tag="pgaptmp_003812"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HGU4197238.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7678..8523
                     /gene="ugpE"
                     /locus_tag="ACHKNB_RS19065"
                     /old_locus_tag="pgaptmp_003813"
     CDS             7678..8523
                     /gene="ugpE"
                     /locus_tag="ACHKNB_RS19065"
                     /old_locus_tag="pgaptmp_003813"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HGU4197239.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8525..9595
                     /gene="ugpC"
                     /locus_tag="ACHKNB_RS19070"
                     /old_locus_tag="pgaptmp_003814"
     CDS             8525..9595
                     /gene="ugpC"
                     /locus_tag="ACHKNB_RS19070"
                     /old_locus_tag="pgaptmp_003814"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HGU4197240.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9592..10335
                     /gene="ugpQ"
                     /locus_tag="ACHKNB_RS19075"
                     /old_locus_tag="pgaptmp_003815"
     CDS             9592..10335
                     /gene="ugpQ"
                     /locus_tag="ACHKNB_RS19075"
                     /old_locus_tag="pgaptmp_003815"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HGU4197241.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10322..10762)
                     /gene="yhhA"
                     /locus_tag="ACHKNB_RS19080"
                     /old_locus_tag="pgaptmp_003816"
     CDS             complement(10322..10762)
                     /gene="yhhA"
                     /locus_tag="ACHKNB_RS19080"
                     /old_locus_tag="pgaptmp_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HGU4197242.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10882..12624
                     /gene="ggt"
                     /locus_tag="ACHKNB_RS19085"
                     /old_locus_tag="pgaptmp_003817"
     CDS             10882..12624
                     /gene="ggt"
                     /locus_tag="ACHKNB_RS19085"
                     /old_locus_tag="pgaptmp_003817"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HGU4197243.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12662..12946)
                     /gene="yrhB"
                     /locus_tag="ACHKNB_RS19090"
                     /old_locus_tag="pgaptmp_003818"
     CDS             complement(12662..12946)
                     /gene="yrhB"
                     /locus_tag="ACHKNB_RS19090"
                     /old_locus_tag="pgaptmp_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HGU4197244.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2864 a         3325 c         3734 g         3038 t
ORIGIN      
        1 tagggatttt aatcgctatt atcacaaaat actgcgctaa acccttaatc agacaggcaa
       61 aaacagtgca gtataaaaaa agaacagtct gatttgttaa cacataaaaa caaagcaaca
      121 caacatcacg aatggggatt tttgactatg aaacggaatg cgaaaactat catcgcaggg
      181 atgattgcac tggcaatttc acacaccgct atggctgacg atattaaagt tgccgttgtc
      241 ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag
      301 gcgattaaag acattaatgc caaaggggga attaaaggcg ataagctggt gggcgtggaa
      361 tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg ccaacaaaat cgttaacgac
      421 ggcattaaat acgttattgg tcatctgtgt tcttcttcta cccaacctgc gtcagatatc
      481 tacgaagacg aaggtattct gatgatctcg ccgggagcga ccaacccgga gctgacccaa
      541 cgcggttatc aacacattat gcgtactgcc gggctggact cttcccaggg gcctacggcg
      601 gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa
      661 cagtatggcg aagggctggc gcgttcggtg caggacgggc tgaaagcggc taacgccaac
      721 gtcgtcttct tcgacggtat taccgccggg gagaaagatt tctccgcgct gatcgcccgc
      781 ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt actacccgga aatggggcag
      841 atgctgcgcc aggcccgttc cgttggcctg aaaactcagt ttatggggcc ggaaggtgtg
      901 ggtaacgcat cattgtcgaa tattgctggc gatgctgccg aaggcatgtt ggtcactatg
      961 ccaaaacgct atgaccagga tccggcaaac cagggcatcg ttgatgcgct gaaagcagac
     1021 aagaaagatc cgtccgggcc ttatgtctgg atcacctacg cggcggtgca atctctggcg
     1081 actgcccttg agcgtactgg cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct
     1141 aacggtgcaa acaccgtgat tgggccgctg aactgggatg aaaaaggcga tcttaaggga
     1201 tttgattttg gtgtcttcca gtggcacgcc gacggttcat ccacggcagc caagtgaaaa
     1261 tcccaccgcc cgtgaaaaac gggcgggttt agaaaggtta ccttatgtct gagcagtttt
     1321 tatatttctt gcagcagatg tttaacggcg tcacgctggg cagtacctac gcgctgattg
     1381 ccatcggcta caccatggtt tacggcatta tcggcatgat caacttcgcc cacggcgagg
     1441 tttatatgat cggcagctat gtctcattta tgatcatcgc cgcgctgata atgatgggca
     1501 ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg cgcaatcgtc atcgccagcg
     1561 cctacggctg gagtatcgaa cgggtagcct atcgcccggt gcgtaactct aagcgcctga
     1621 ttgcgctcat ctcggcaatc ggcatgtcca tcttcctgca aaactacgtt agcctgactg
     1681 aaggttcgcg tgacgtggcg ctgcccagtc tgtttaacgg tcagtgggtg gtggggcata
     1741 gcgaaaactt ctctgcctct attaccacca tgcaggcggt gatctggatt gtcaccttcc
     1801 ttgccatgct ggcgctgacg gttttcattc gctattcccg tatggggcgc gcgtgtcgtg
     1861 cctgcgcgga agacctgaaa atggcgagcc tgcttggcat taacaccgac cgggtgattg
     1921 cgctgacctt tgtgattggc gcggcgatgg cggcggtggc aggtgtgctg ctcggtcagt
     1981 tctacggcgt aattaacccc tacatcggct ttatggccgg gatgaaagcc tttaccgctg
     2041 cggtgctcgg tgggattggc agcattccgg gagcgatgat tggcgggctg attctgggga
     2101 ttgcggaggc gctctcttct gcctatctga gtacggaata taaagatgtg gtctcattcg
     2161 ccctgctgat tctggtgctg ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag
     2221 agaaagtatg aaaccgatgc atattgcaat ggcgctgctc tctgccgcga tgttttttgt
     2281 cctagcgggc gtctttatgg gcgtgcaact ggagctggat ggcaccaaac tggtggtcga
     2341 cacggcttca gacatccgtt ggcagtgggt gtttatcggc acggcggtgg tctttttctt
     2401 ccagcttttg cgaccggctt tccagaaagg gctaaaaagc gtttccggac cgaagtttat
     2461 tctgcccgcg attgatggct ccacggtgaa gcagaaacta ttcctcgtgg cgctgctggt
     2521 gcttgcggtg gcgtggccgt ttatggtgtc gcgtgggacg gtggatatcg ccaccctgac
     2581 catgatctac attatcctcg gcctcgggct gaacgtggtt gttggtcttt ctggtctgct
     2641 ggtgctgggg tacggcggct tttacgccat cggcgcttac acttttgcgc tgctcaatca
     2701 ctattacggc ctgggcttct ggacctgtct gccgattgcc gggttaatgg cagcggcggc
     2761 cggcttcctg ctcggcttcc cggtgcttcg cttgcgcggc gactatctgg cgatcgttac
     2821 cctcggtttc ggcgaaattg tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg
     2881 cccgaacgga atcagtcaga tcccgaaacc gacactcttc ggactcgagt tcagccgtac
     2941 cgctcgtgaa ggcggctggg acacgttcag taatttcttt ggcctgaaat acgatccctc
     3001 cgatcgtgtc atcttcctct acctggtggc gttgctgctg gtggtgctaa gcctgtttgt
     3061 cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagacga
     3121 gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc aagctgactg cctttaccat
     3181 aagtgccgcg tttgccggtt ttgccggaac gctgtttgct gcgcgccagg gctttgtcag
     3241 cccggaatcc ttcacctttg ctgaatcggc gtttgtactg gcgatagtgg tgctcggcgg
     3301 tatgggatcg cagtttgcgg tgattctggc ggcaattttg ctggtggtat cgcgcgaatt
     3361 gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt ggtttgatgg tgctgatgat
     3421 gatctggcgt ccgcagggct tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg
     3481 cgcagcgaaa ggagagcagg catgagtcag ccattattat ctgttaacgg tctgatgatg
     3541 cgcttcggcg gcttgctggc ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc
     3601 gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg tttttaactg cctgaccgga
     3661 ttctacaaac ccaccggcgg cactatttta ctgcgtgatc agcatctgga aggtttaccg
     3721 gggcagcaaa ttgcccgcat gggcgtggtg cgtaccttcc agcatgtgcg tctgttccgt
     3781 gaaatgacgg tgattgaaaa cctgctggtg gcgcagcatc aacaactgaa aaccgggctg
     3841 ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc agagcgaagc gctcgaccgc
     3901 gccgccacct ggcttgagcg cattggtttg ctggaacacg ccaaccgtca ggcgagtaac
     3961 ctggcctatg gtgaccaacg ccgtctggag attgcccgct gcatggtgac gcagccggag
     4021 attttaatgc tcgacgaacc ggcggcaggt cttaacccaa aagaaaccaa agagctggat
     4081 gagctgattg ccgaactgcg caatcatcac aacaccacta tcttgttgat tgaacacgat
     4141 atgaagctgg tgatggggat ttcagaccga atttatgtgg tcaatcaggg gacgccactg
     4201 gcaaacggta cgccggagca aatccgtaat aacccggacg tgatccgtgc ctatttaggt
     4261 gaggcataag atggaaaaag tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa
     4321 aatccaggcg ctgcatgagg tgagcttgca tatcaatcag ggcgagattg tcacgctgat
     4381 tggcgcgaac ggggcgggga aaaccacctt gcttggtacg ttatgcggcg atccgcgcgc
     4441 caccagcggg cgaattgtgt ttgatgataa agacattacc gactggcaga cggcgaaaat
     4501 catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt
     4561 ggaagagaac ctggcgatgg gcggcttctt tgccgaacgt gaccagtttc aggagcgcat
     4621 aaagtgggtg tatgagctgt ttccacgtct tcatgagcgt cgtgttcagc gggcgggcac
     4681 catgtccggc ggtgaacagc agatgctggc gattggtcgc gcgctgatga gcaacccgcg
     4741 tttgttgttg cttgatgagc catcgctcgg tcttgcgccg attatcatcc agcaaatttt
     4801 cgacaccatc gagcagctgc gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc
     4861 caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct
     4921 ctccgatacc ggcgacgcgc tgctggcgaa cgaagcagtg agaagtgcgt atttaggcgg
     4981 gcaataacac gttgattgat aaggagtcaa aagactcctt tgagacaggt gacaaatgta
     5041 aaattgcctg atgcgctgcg cttatcaggc ctactgggtg agttgcaata tattgaattt
     5101 gcacgatctt gtaggccgga taaggcgttt acgccgcatc cggcatgaaa cgatgagtaa
     5161 tctgtagagt ttgattcaga ccttctattt tccgcttatc cgtgccccat ctcccatttt
     5221 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
     5281 aacaaaaatg ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
     5341 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
     5401 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
     5461 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
     5521 tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
     5581 aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt
     5641 tatgaagttg gcaccgccac catgatggca tcgaaagcca ttaaaccggt atatgacgtg
     5701 tttaaagagg cggggattca attcgatgag tcgcagtttg tgccgacggt ttccggttac
     5761 tactccgaca gcaaaacggg ccacttactc tcccagccat tcaacagctc gacccccgtt
     5821 ctctattaca acaaagacgc cttcaagaaa gcaggattag acccggaaca accgccgaaa
     5881 acctggcagg atctggcgga ctatgccgcg aaactgaaag cctccggtat gaagtgcggc
     5941 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
     6001 ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag
     6061 ccggagcagg tgaaacacat cgccatgctc gaggagatga acaagaaggg cgacttcagc
     6121 tacgtcggtc gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc
     6181 accgcctctt ccggttctct cgctaatatt cgtgagtacg ccaaatttaa ctatggcgta
     6241 ggcatgatgc cttacgacgc cgatgcgaaa gatgcaccgc aaaacgccat tatcggcggg
     6301 gccagcctgt gggtgatgca gggcaaaaat aaagaaacgt ataccggcgt ggcgaagttc
     6361 ctcgacttcc tcgcgaagcc agaaaacgct gccgagtggc atcagaaaac cggttatctg
     6421 ccaatcacca aagcagcgta tgacctgacc cgtgagcagg gcttttatga gaaaaaccca
     6481 ggggcggata ccgcgacgcg tcagatgctg aataagccgc cgttgccgtt caccaaaggg
     6541 ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct ggagagcgtg
     6601 tggaccggta agaagacacc acagcaggca ctggataccg ccgttgagcg tggaaatcag
     6661 ttgctgcgcc gctttgagaa atcgacgaag tcttaatcag tgtaatgtcg gatgcgtttc
     6721 gcttatctga cctggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca
     6781 ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct
     6841 gctggtcgcg ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc
     6901 gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggcct
     6961 ggataacttc gtcgcgctgt ttcatgacag ctactatctc gactccttct ggacgacgat
     7021 aaaattcagc acctttgtca ccgtcagcgg tttgctggtg tcgctgttct tcgcggcgct
     7081 ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc ttaatgttgc tgccttacgc
     7141 cgtggctccc gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct
     7201 gatcacccat tttctcgcgg agttcggcta cgactggaac cacgcgcaaa acagcggtca
     7261 ggcaatgttt ctggtggtgt ttgcctcagt atggaagcaa atcagctaca acttcctgtt
     7321 cttctatgcc gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg
     7381 tgcagggccg attcgccgct tctttaagat tgcgctgccg cttatcgccc cggtgagttt
     7441 cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc
     7501 cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgcga
     7561 aggttttacc ggactggatc tggcttcgtc tgccgcacag tcggtggtgt tgatgttcct
     7621 cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg
     7681 attgagaacc gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg
     7741 gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc
     7801 tatgccgcgc cgatgacgct catccccggc acacatctgc tggaaaacat ccacaacatc
     7861 tgggtgaacg gggtaggcac gaatagcgcg ccgttctggc ggatgttgct taacagcttt
     7921 gtgatggcgt tcagcattac gctcggcaaa attaccgtct cgatgctctc ggcatttgcc
     7981 attgtctggt ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg
     8041 atgctgccgg ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgcagatg
     8101 ctcgacagct acgccggttt aacgctgccg ctgatggcct cggcgaccgc tactttcctg
     8161 ttccgccagt tctttatgac gctgccggat gagctggtgg aagccgcgcg gatcgacggc
     8221 gcatcgccaa tgcgcttctt ttgcgacatc gtttttccgc tctccaaaac taatctggcg
     8281 gcgctgtttg tgatcacctt tatctacggc tggaatcagt atttgtggcc gttgttgatt
     8341 attaccgacg ttgatctcgg caccaccgtg gcagggatca aagggatgat cgccactggc
     8401 gaaggcacca cggaatggaa ctcggtaatg gcggcgatgt tgttaacgct tatccctccg
     8461 gtggtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa
     8521 taagatggca ggactgaaat tacaggcagt aaccaaaagc tgggatggca aaacccaggt
     8581 gattaaacca ctgacccttg atgtggcgga tggcgaattt atcgtgatgg tcgggccgtc
     8641 tggctgcgga aaatcgacgc tgctgcgcat ggttgccggg ctggagcggg tgacggaagg
     8701 cgatatctgg atcaacgacc agcgcgtgac cgaaatggag ccgaaagatc gcgggattgc
     8761 gatggtgttc cagaactacg cgctttatcc gcatatgagc gtcgaagaaa acatggcgtg
     8821 ggggctgaaa attcgcggca tgggcaagca gcaaatagcc gagcgcgtta aagaagcggc
     8881 gcgcattctg gagctggatg gcctgcttaa gcgccgcccg cgcgagctat ccggcggtca
     8941 gcgtcagcgt gtggcgatgg gtcgcgcgat tgtgcgcgat ccggcggtgt tcctgtttga
     9001 tgagccgctc tctaacctcg atgccaagct gcgcgtgcag atgcgccttg agctgcaaca
     9061 gctgcaccgt cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaagcgat
     9121 gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt gccgaacaga ttggcacacc
     9181 agttgaagtc tacgaaaagc ccgccagcct gtttgtagcg agttttatcg gcagtccggc
     9241 gatgaacctg ctgacaggcc gcgtgaataa cgaaggcacg catttcgaac tggacggcgg
     9301 tattgagctg ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgactctcgg
     9361 cattcgcccg gaacatattg cgctaagctc gcaggcagaa ggcggcgtac cgctggtgat
     9421 ggacacgctg gagatcctcg gcgcagataa cctggcgcac ggacgctggg gcgagcagaa
     9481 gctggtggtg cgactggcgc atcaggagcg cccgacggca ggcagcacgc tgtggctgca
     9541 tctggcggaa aatcagctgc atctttttga tggtgaaaca ggacaacgag tatgagtaac
     9601 tggccttatc cccgcatcgt cgctcatcgt ggcggcggta agctggcccc ggaaaacacc
     9661 ctggcggcaa tcgacgtcgg ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag
     9721 ttatcgaaag atggcgagat cttcctgctc catgacgaca atctcgaacg caccagcaac
     9781 ggctggggcg tcgcgggtga actgaactgg caggatttac tgcgcgtgga tgcgggcagt
     9841 tggtacagca aagcgtttaa aggtgagtcg ctgccgttgc tttcgcaggt ggcggaacgc
     9901 tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac ccaccactgg cactggacca
     9961 ttaacgggca aaatggtggc gctggcggca cgccaactgt gggccggtat gacgccgccg
    10021 ctgctgtcat cgtttgagat tgatgcttta gaagctgcac agctggcggc accggaactg
    10081 ccgcgtggtt tattgcttga tgagtggcgc gacgactggc gcgaactgac cgcgcggctg
    10141 ggctgcgtct ctattcatct caatcataag ttactcgata aagtgcgagt gatgcagttg
    10201 aaagacgccg gactgcggat tctggtttat accgtcaata aaccccagcg cgcggcagag
    10261 ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga ttgacgtgat tggtccgaac
    10321 tttacggccc aatagttttc aacggaatgt caggctgcgg cgtgctgggc tgattcagca
    10381 tgtcgccgtt tctcctctcc ggcagcatat gctgctgatt caacgaacta tccggattac
    10441 ggttgctgtt taacatcccg ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc
    10501 gctcccccgg ctgtgactgc aacacccgct gagaattatt gtttatctgg ttttctaaat
    10561 gctgctgttg caactgagtt tgcgttttca gttgctgatt cagcatccct ttttgctgga
    10621 tttgctgagt ctgcatctgg gtttgcatcc gctgctggct gggtatctga taccccggct
    10681 ggttagggtt gttcagagta ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg
    10741 taagaatcag aagtcgtttc atcgcgtatc ctcctctgaa gatatccttt aagtttactc
    10801 gcttcccgac aaaacgatga ttaattcaga gttatatacc aggcttagct ggggttgccc
    10861 cttaatctct ggagaataac gatgataaaa ccgacgtttt tacgccgggt ggccattgct
    10921 gctctgctct caggaagttg ttttagcgcc gccgccgcgc ctcctgcgcc gcccgtctcg
    10981 tatggtgtgg aggaagatgt cttccacccg gtacgcgcga aacagggaat ggtagcgtct
    11041 gtggacgcca ctgccactca ggtgggggtg gatattctca aggagggcgg gaatgccgtt
    11101 gatgccgccg tggcggtggg ctacgcgctg gcggtaacgc atccgcaggc agggaatctg
    11161 ggcggtggtg gttttatgct gatccgctcg aaaaatggca ataccaccgc tatcgatttc
    11221 cgcgaaatgg cacccgctaa agcgacccgc gatatgttcc ttgatgacca gggcaacccg
    11281 gacagcaaaa aatcactcac ttcgcatctg gcttccggca caccaggtac ggtcgctggt
    11341 ttctcgctgg cgttggataa gtacggcact atgccgttga acaaagtggt gcagcccgcg
    11401 ttcaaactgg cacgcgatgg ttttatcgtt aacgatgctt tggcagacga tctcaaaacc
    11461 tacggtagcg aagtgttgcc gaatcacgaa aacagcaaag ctatcttctg gaaagagggc
    11521 gaaccgctga aaaagggcga cacgctggtg caggcgaacc tggcaaagag cctggagatg
    11581 attgctgaaa acggcccgga cgaattctat aaaggcacga ttgcggaaca gatcgcccag
    11641 gagatgcaga aaaacggtgg cttgatcact aaagaagatt tagcggccta taaagcggtc
    11701 gaacgcactc cgataagcgg cgattatcgc gggtatcagg tttactccat gccaccgcca
    11761 tcctccggcg ggatccatat cgtacaaatc ctcaatattc tggaaaactt cgatatgaag
    11821 aaatacggct ttggcagcgc cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc
    11881 tacgccgacc gctcggaata tcttggcgac ccggattttg tcaaagtccc gtggcaggca
    11941 ctgaccaata aagcctatgc caaatccatt gccgatcaaa ttgatatcaa caaagcgaag
    12001 ccgtccagtg agattcgccc tggcaagctt gcgccttatg agagtaatca aactacccat
    12061 tactctgtag tggataaaga cggtaacgcg gtggcggtga cctatacgct gaacaccacc
    12121 ttcggtacgg gtattgtcgc gggcgagagc ggtattctgc ttaataacca gatggatgat
    12181 ttctccgcca aaccgggcgt accgaacgtt tacgggctgg tgggcggtga tgccaacgcc
    12241 gtcgggccga acaaacgccc gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt
    12301 aaaacctggc tggttaccgg tagcccaggc ggtagccgga tcatcactac agtgctgcaa
    12361 atggtggtga atagcatcga ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt
    12421 ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa aagggtttag cccggatacg
    12481 ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga aagaggcgat gggcagtaca
    12541 caaagcatta tggttgggcc ggacggtgag ttgtacggcg catccgaccc gcgctcggtg
    12601 gatgatttaa cggcggggta ctaaggttag cggccctctt cgtgggaaga gggctatttt
    12661 gtcagggcaa gccgaaggta gccttcttta tttcgtaatc ctgtagatat tcttccagcg
    12721 gctttgccgt tcccagagaa tgaatttcac cactgtcttt atcaataata aaaggtgcgt
    12781 taccagctaa gcgcgcggcc tcatctccag tttcgagaaa ttctcgtgct tcgaaacaga
    12841 aataccagcc ctggctaaag cgtccatgta gagtaatgac gaccgggaga tctgcatcat
    12901 caaggtaatg gttcgctttc gcgaatgcgt cgtgataagt aatcataatt ataataaata
    12961 t
//
LOCUS       DAZFVO020000139        12790 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFVO020000139 DAZFVO020000000
VERSION     DAZFVO020000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.140"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..369
                     /locus_tag="ACHKNB_RS19095"
                     /old_locus_tag="pgaptmp_003819"
     CDS             <1..369
                     /locus_tag="ACHKNB_RS19095"
                     /old_locus_tag="pgaptmp_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4197245.1"
                     /translation="SGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVT
                     ARAGLGYQFDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEK
                     SAFGKYNVDNAVNASFRYSF"
     gene            complement(936..3608)
                     /gene="rcsD"
                     /locus_tag="ACHKNB_RS19100"
                     /old_locus_tag="pgaptmp_003820"
     CDS             complement(936..3608)
                     /gene="rcsD"
                     /locus_tag="ACHKNB_RS19100"
                     /old_locus_tag="pgaptmp_003820"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HGU4197246.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            4347..5450
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS19105"
                     /old_locus_tag="pgaptmp_003821"
     CDS             4347..5450
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS19105"
                     /old_locus_tag="pgaptmp_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4197247.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSARGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            5562..6617
                     /gene="apbE"
                     /locus_tag="ACHKNB_RS19110"
                     /old_locus_tag="pgaptmp_003822"
     CDS             5562..6617
                     /gene="apbE"
                     /locus_tag="ACHKNB_RS19110"
                     /old_locus_tag="pgaptmp_003822"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HGU4197248.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGKPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     IGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPKKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            6691..7755
                     /gene="ada"
                     /locus_tag="ACHKNB_RS19115"
                     /old_locus_tag="pgaptmp_003823"
     CDS             6691..7755
                     /gene="ada"
                     /locus_tag="ACHKNB_RS19115"
                     /old_locus_tag="pgaptmp_003823"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HGU4197249.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFHPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            7755..8405
                     /gene="alkB"
                     /locus_tag="ACHKNB_RS19120"
                     /old_locus_tag="pgaptmp_003824"
     CDS             7755..8405
                     /gene="alkB"
                     /locus_tag="ACHKNB_RS19120"
                     /old_locus_tag="pgaptmp_003824"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HGU4197250.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGELRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            8481..10124
                     /gene="yojI"
                     /locus_tag="ACHKNB_RS19125"
                     /old_locus_tag="pgaptmp_003825"
     CDS             8481..10124
                     /gene="yojI"
                     /locus_tag="ACHKNB_RS19125"
                     /old_locus_tag="pgaptmp_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HGU4197251.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            10342..11988
                     /gene="mqo"
                     /locus_tag="ACHKNB_RS19130"
                     /old_locus_tag="pgaptmp_003826"
     CDS             10342..11988
                     /gene="mqo"
                     /locus_tag="ACHKNB_RS19130"
                     /old_locus_tag="pgaptmp_003826"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HGU4197252.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(12137..12646)
                     /gene="eco"
                     /locus_tag="ACHKNB_RS19135"
                     /old_locus_tag="pgaptmp_003827"
     CDS             complement(12137..12646)
                     /gene="eco"
                     /locus_tag="ACHKNB_RS19135"
                     /old_locus_tag="pgaptmp_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HGU4197253.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         3161 a         3089 c         3325 g         3215 t
ORIGIN      
        1 tccgggaaac agttcagctg gaaggaccag ggaatgaacc tcaccatgaa ggataaggac
       61 tttaatccgc tgattggccg taccggtgtg gatgtgggta aatctttctc cggtaaggac
      121 tggaaagtga cggcacgtgc tggtcttggc tatcagtttg acctgctggc gaacggagaa
      181 acggtactgc gtgatgcatc cggagagaag cgtattaagg gcgagaaaga cggccgtatg
      241 ctgatgtctg tgggtctgaa cgcagaggtc cgtgacaaca tccgttttgg tctggagttt
      301 gagaaatcag cctttggtaa gtacaacgtt gataatgctg tcaacgccag cttccgttat
      361 tcattctgac ggttgttact gaaggggatt atggcctgac ggctccctgt aaataaagac
      421 aggcacagaa gattgtgagt tactcagtgt tccaccaaaa agtaaggtcg caactctgtg
      481 cctgcttcgt aagtgtacag tgagggccat atggtcataa ctgaagcatc cgatgatgtg
      541 gtgtctacca gagtgtcaga tatgcaggaa aatatgagtt ccgacagaaa tgcagtaaaa
      601 aaggagtctc gctctcccct ccagaaaact ccagggctgt aagttgtatt aaaaagctga
      661 tttttcttta gcgatacaga cctcaataag tataacaccc ttcagttact gtggttatgt
      721 acaggtgtta tttttaacag tgtggataaa cagtgtcgat tattcagatt ggttatgggg
      781 catgtatggg acatttttaa tcggcgaaat acgtcgaagt tcgtcgatat ggtaaacgaa
      841 tcatctatcc aacccttgaa aaacggcgct cctggacgat cttcgtcgat ttttaaaatg
      901 caacaagtac gttgttcatg taataggcta ccttgctaca gcaagctctt gacataactg
      961 tcaatgtcgc tgatgtattt ttctattcct ggaacatcct tctcacgaat cagatgttcc
     1021 agcgtttcac ataactgctt gccgggtacc agatttagca tggcaaatac gcctttaaga
     1081 cgatgagccg tttgggctaa cgcagcaaag tcactggttg ctgcttcagt atacagcctc
     1141 ttaacatcat ccggtactgt gtctacaaag agcgcataat agccgctggc atggagttgc
     1201 gcattttcat ctccgcccag aggcgattct gtcacctctt cctgcgccag ttgcacttca
     1261 attaattgta agaccgcttc ctgcatagcg ttgctcatat tgaagttgac gcacaattga
     1321 ccaggcccaa tttcccgtac gccagactca tcatcgctta aaagcaagcc agaggcagta
     1381 agattagacg gattatccgt taaaaagata tcataatctt gactaattaa tctttcatcg
     1441 ggtgtgatac aggttgcacc ccaattttct aactggcgag tgacaatatt ccgaatttct
     1501 gccgaagtaa catccaccat tacgcagaca tcatccagta aacgctcttc ttcctcttca
     1561 acttccgggt cagctgcgag cattttgata tgcacagagt agcgtgtacc aagcccatcc
     1621 cgcgttttga tgtttaaatg accgcccagt ttacgtgcca gttgatcgct cagccagaat
     1681 gccagcgggt ccgccttgcc atagcgatcg ttttgggtct ggttgataaa cgggaagtgc
     1741 aaattatcca tttcatgaat acttacgcct tctcccgtgt ccagaatgcg gaacgtcagg
     1801 cggtcttcgg aggactcatc ctgatcaacc tcaagggtga tttttcccaa ttgcgttgag
     1861 gtcacggcat attgcatcag tagcagcaaa atacgtcgta aggcatcgcg atcgccgcgg
     1921 cgcatatcgt gtgctttcag atgattgtta atcagcagtt gcagaccttt acgcttgatg
     1981 gcaggcaaca ctgaaggcac aacttcatca attaaatcct gcacggagaa cagcaccgtc
     2041 tcacttttcc agctatcatc cgcaagcatg ttcgctaact gtatttcatc aaccagccgc
     2101 accagcacat ctgcctgatt cgccagttgt ttgctttccg gggcgttgag tttagccgcg
     2161 ctctccgcca gggactgtgc gggttctttc agcgcatcgc caatgttttt cataaaggtc
     2221 atccgcccct gctggttttt ctcatacaga cgctgcgcct gcttgagttt cttgtttacc
     2281 agcacttcgc gatcctgatc gcgaataatg aaaatttgtg tgcgcggcgc gacctggctg
     2341 cggaacatgc ggatctcata cagctcgtta ttgatcgtcg cctgaataat cccctgatgc
     2401 tgttccgcca tggtggtgat gttttgcaga ttcaaatgcg gcagcaaatg atcggcaatt
     2461 ttgttactta tgacagtgcg gttcgattcc tgatcgtgaa ccagcaggcc gagcggcagc
     2521 agtgagacta tctcttcatt aattgcccgt aaaatgcgca attcgttatt gaccgccgtg
     2581 ctgggtagac tttctgtact gcggctggag aaatggcgga atgtggtata gccaaataac
     2641 gccagcgcca gcaaaccgat gttcagcagc agtggcagca gaatgttttg caacgtatcc
     2701 agcaataagg tgccataagg aacctgccag accaggcgca tatcggtaga gttgagtgcc
     2761 gaggagattt caatcttcgt actgttaaag tggatactga cgctatccgt cccttctttc
     2821 tcattatcat tgtttcccgt tgccgtcgcg tctggctcaa ggcggaaact gtccagcggc
     2881 atacccggtg ggatcaaatc attaatcggc agatcaaaag ccacgaccgt tgccagatgt
     2941 cctggctggt taaaggtagt acgcaaggta aagtaatgac cgttctgcca ggccaggcgg
     3001 cgcatgttag aaaagctttc gcgttcatcg agggcgttgg cctgttgcaa catctctgca
     3061 cgacgtgaat caacaatgtc gctgacggtc gattctttaa atccggaggt gagatcttta
     3121 aggggcaggg ttgagatcag caccagactg ttatcctgac cattcaggta atacatcgac
     3181 cacggtacat tttctgcgcc ccacaatgtg tccagatagg tggacatccg ctgagtcatc
     3241 tcaagcgttg agttgtcgtg agagccaaag attaacgctt ccgttttgcg gcgcggtttc
     3301 tccagatagt agacatcctg tttcaggcgc gtctcttgta aaccttcgcc ggaggagggg
     3361 gaggtcgtcg cggcaatgtt gtcgtagatc tgccaggtca cgtaacgcca ggtatcgacg
     3421 cgcttttgga tagcgtgggt aatgtcgaca atctggtaac ttttatcttt cagccatgcg
     3481 ttaacggcgc tttgtaccat tacacccatc gtcaccagta acacaatgat caacagtaaa
     3541 aagaagcggg taatgctccc cggtaggagt gaaaagcggg tcgtggccgt tgtctctttc
     3601 tgacgcattc gtgtttatga cctgttaaaa cttcgcgaag tgatagggca gtataaaggg
     3661 tacagtgtga aattccagac tcttacctga ttgatgattc tctattctcc cgtgatattt
     3721 atcatcctgc attgggggaa atagatgtac aagcgccatt ttgctgtaca aataacctac
     3781 aaaaagccca acgaaaataa ttgcaaataa acaaagattg ctggaaatta tgctgatgtt
     3841 aattatttgt gaaatagtta acaagcgtta tagtttttct gtggtagcaa agaataatga
     3901 aaagtgtgta aagaagggta aaaaaaaccg aatgcgaggc atccggttga aataggggta
     3961 aacagacatt cagaaatgaa tgacggtaat aaataaagtt aatgatgata gcgggagtta
     4021 ttctagttgc gagtgaaggt tttgttttga cattcagtgc tgtcaaatac ttaagaataa
     4081 gttattgatt ttaaccttga attattattg cttgatgtta ggtgcttatt tcgccattcc
     4141 gcaataatct taaaaagttc ccttgcattt acattttgaa acatctatag cgataaatga
     4201 aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt ttggggagaa
     4261 tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa aaagcaaata
     4321 aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc ctggtcccag
     4381 ctctgctggt agcaggcgca gcaaacgctg ctgaagttta caacaaagac ggcaacaaat
     4441 tagatctgta cggtaaagta gacggcctgc actatttctc tgacgacaag tctgtagatg
     4501 gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact gaccagctga
     4561 ccggttacgg ccagtgggaa tatcagattc agggcaacag cgctgaaaac gaaaacaact
     4621 cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct ttcgactacg
     4681 gtcgtaacta cggcgttgtt tacgacgtaa cttcctggac cgacgtactg ccagaattcg
     4741 gtggtgacac ctacggttct gacaacttca tgcagcagcg tggtaacggc tttgcgacct
     4801 accgtaacac tgacttcttc ggtctggttg acggcctgaa ctttgctgtt cagtaccagg
     4861 gcaaaaacgg tagcgctaga ggcgaaggca tgaccaacaa tggtcgtgat gctctgcgtc
     4921 agaacggcga cggcgttggc ggttctatca cttatgatta cgaaggcttc ggtatcggtg
     4981 ctgcagtttc cagctccaaa cgtactgatg ctcagaacac cgctgcttac atcggtaacg
     5041 gcgaccgtgc tgaaacctac actggtggtc tgaaatacga tgctaacaac atctacctgg
     5101 ctgctcagta cacccagacc tacaacgcaa ctcgcgtagg ttccctgggt tgggcgaaca
     5161 aagcacagaa cttcgaagct gttgctcagt accagttcga cttcggtctg cgcccgtctg
     5221 tagcatacct gcagtctaaa ggtaaaaacc tgggcaccat cggtactcgt aactacgacg
     5281 acgaagatat cctgaaatat gttgatgttg gtgcgaccta ctacttcaac aaaaacatgt
     5341 ccacctacgt tgactacaaa atcaacctgc tggacgacaa ccagttcact cgtgacgctg
     5401 gcatcaacac tgataacatc gtagctctgg gcctggttta ccagttctaa tctcgattga
     5461 tctcgaacaa agggcctgcg ggcccttttt tcattgtttt cagcgtacaa acacagtttt
     5521 ttggtgtact cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc
     5581 gcgtggcact gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg
     5641 ccaaaaccca cgctactgaa gttaccgttc ttgaaggcaa aaccatgggt accttctggc
     5701 gtgccagcat cccgggcatt gacgccaaac gcagtgccga acttaaagaa aagattcaga
     5761 cccagctgga tgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc
     5821 gctttaacga ctcgcaaagt ttgtcgccgt ggccggtaag tgaagcgatg gccgatatcg
     5881 tcaccacctc gctgcgcatt ggcgcaagga ccgatggtgc gatggatata accgtcgggc
     5941 cgctggtgaa tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg
     6001 aacagatcga tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc atcaatcagt
     6061 cgcatcagca atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg
     6121 gtgaaggtta tgcggcggat cacctggcac gcttgatgga gcaggaaggg atttcccgct
     6181 atctggtgtc ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggcaagccgt
     6241 ggcgggtagc gattcaaaaa ccaaccgata aagaaaacgc ggttcaggct gtggtggata
     6301 tcaacggtca tgggattagc acctctggca gctaccgtaa ctattacgaa ctggacggca
     6361 aacgtctttc ccatgttatc gatccgcaaa tcgggcgtcc catcgaacac aatctggtat
     6421 ccgtgacggt gattgctccg acggcgctgg aagccgatgc ctgggatact ggcttgatgg
     6481 tactcgggcc gaagaaagcc aaagaggttg ttcgccggga agggctggcg gtttatatga
     6541 tcaccaaaga aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg
     6601 tcagcgaaaa aaattaaagc gcaagattgt tggtttttgc gtgatggtga ccgggcagac
     6661 taaaggctat ccttaaccag ggagctgatt atgaaaaaag ccacatgctt aactgacgat
     6721 caacgctggc aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc
     6781 gtgcgtacca caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa
     6841 aacgtctcct tctacgcaaa tgccagcgag gcactcgccg ctggctttca cccctgcaaa
     6901 cgttgtcagc cagaaaaagc caatgcccag caacatcggt tggataaaat cacccacgcg
     6961 tgtcgactgc tggaacagga aacgcctgta acgctggaag ccttagccga ccaggtggcg
     7021 atgagtccat ttcatctaca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc
     7081 tggcaacagg cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg
     7141 acgacgtcta ttcttaacgc cggattcccc gacagcagca gttactatcg caaagctgac
     7201 gaaacgctgg gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt
     7261 tacgcgctgg ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt
     7321 tgcgcgatat tgctgggcga tgatgacgcg acactaatca gcgagttgca gcagatgttt
     7381 cccgctgccg acaacgcgcc tgccgatctg atgtttcagc aacatgtgcg tgaagtgatc
     7441 gccagcctca atcaacgcga tacgccgctg acgttaccgc tggacattcg cggcactgct
     7501 tttcagcaac aagtctggca ggcactgcgc acgatacctt gcggtgaaac cgtcagttat
     7561 cagcaactgg ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt
     7621 gccgccaaca agctggctat cataataccc tgtcatcggg tggtccgtgg tgatggcaca
     7681 ctttccggtt accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa
     7741 aatgaggaga ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg
     7801 cggctggagc ggtaattttg caccgttttg cttttaacgc tgcggagcaa ctgatccgcg
     7861 atattaataa cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata
     7921 ccatgtcggt ggcgatgacc aactgtgggc atctgggctg gacgagccat cggcaaggtt
     7981 atctctattc gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccacagagtt
     8041 ttcataattt atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg
     8101 cttgtcttat caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg
     8161 aaccggatct gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat
     8221 ttggtggcct gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg
     8281 tggtatgggg cggtgaattg cggctgtttt atcacggtat tcaaccgttg aaagcggggt
     8341 ttcatccact caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag
     8401 aataaaaata agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat
     8461 tcattgtccg ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc
     8521 catttatcag tgtgatggcg ctaagcctcg ccagtgcggc attaggcatt ggcttaattg
     8581 cttttatcaa tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt
     8641 ttcttggatt attactgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca
     8701 ctttggggca tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata
     8761 cccacgtcga gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg
     8821 atgtgcgcaa tatcaccatt gcttttgtgc gtcttccgga actggtgcag gggatcattc
     8881 tcactatcgg ttccgcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg
     8941 ctatctggat ggcgatcacc atctggggtg gttttgtact ggtggcgcgg gtgtacaaac
     9001 atatggcgac cctgcgtgaa accgaagaca agctgtacac ggattttcaa accgtactcg
     9061 aagggcgcaa agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct
     9121 acatccccga tgcgcaagag tatcgccatc atattatccg cgcagacacc ttccatctta
     9181 gcgccgtgaa ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg
     9241 cgaacagtct cggttgggct gataccaacg ttgctgcgac ctattcgttg acgctgttat
     9301 tcctgcgtac gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg
     9361 tggcgtttaa caagctgaac aaattcgcgc tcgcgccttt caaagctgag tttccgcgcc
     9421 cgcaggcttt tcccaactgg caaacgctgg agctgcgtaa cgtgacgttt agctatcagg
     9481 ataacgcgtt ttccgttggg ccgattaacc tcaccattaa acgtggcgag ctgctgtttc
     9541 tgattggtgg caacggtagt ggaaaatcga cgctggcgat gttgctgacg ggcttgtacc
     9601 agccacaaag cggcgaaatc ttgctggatg gcaaacctgt cagtggcgaa caaccggaag
     9661 attatcgcaa actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg
     9721 ggccggaggg taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa
     9781 tggctcataa gcttgagtta agcaacgggc gtattgttaa cctgaagtta tcaaaagggc
     9841 agaaaaaacg cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg
     9901 atgaatgggc ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc
     9961 cgctgatgca ggagatgggt aaaactattt tcgctatcag tcatgatgat cattacttta
    10021 tccacgccga ccgcctgctg gaaatgcgca atgggcaact tagcgagctg acgggcgaag
    10081 agcgcgatgc cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc
    10141 tggcggactg ctgccgtcag gtcaatatgc taaaaatccc ttcatttcta ataaccctat
    10201 aattaatcaa cgaaattata atgtttctaa aattagaata taatttataa acattattta
    10261 aatgttgtta cttaagtgtt accgttgatg ccgcgcaaat ctcacctgca ataaagcgac
    10321 taaaagtaag gcattaacaa gatgaaaaaa gtgactgcca tgctcttctc gatggccgtg
    10381 gggcttaatg ccgtttcgat ggcggcaaaa gcgaaagcgt ccgaggagca ggaaactgat
    10441 gtactgttga ttggcggcgg cattatgagc gccacgttgg ggacctattt acgcgagctg
    10501 gagcctgaat ggtcgatgac catggtggag cgcctggagg gtgtcgcgca ggagagttcg
    10561 aacggctgga ataacgccgg aaccgggcat tctgcactga tggaactgaa ctacaccccg
    10621 caaaacgccg atggcagcat cagtattgaa aaagcagtcg ccattaacga agcatttcag
    10681 atttcccgcc agttctgggc gcaccaggtt gagcgcggcg tgctgcgtac tccgcgttca
    10741 tttatcaata ccgttccgca tatgagcttt gtctggggcg aggataacgt taatttcctg
    10801 cgcgcccgtt acgccgcgtt gcaacaaagc tcgctgtttc gcggtatgcg ttactctgaa
    10861 gatcacgcgc agatcaaaga gtgggcaccg ttagtgatgg aagggcgcga tccacaacag
    10921 aaagtagcgg caacacgcac agaaattggc actgatgtga actatggcga gatcacccgc
    10981 cagttaattg cttccttaca gaagaaatct aacttctcgc tgcaactcag cagcgaagtc
    11041 cgcgccctaa agcgtaatga cgataacacc tggaccgtta ccgttgccga tctgaaaaac
    11101 ggcactgcac agaacatccg cgccaaattt gtctttatcg gcgcgggcgg tgcggcgctg
    11161 aagctattac aggaatcggg gattccggaa gcgaaagact acgccggttt cccggtgggc
    11221 ggacagttcc ttgtttcgga aaacccggac gtggttaatc accatctggc gaaggtttac
    11281 ggtaaagcat ccgttggcgc accaccgatg tcggttccgc atatcgatac ccgcgttctg
    11341 gacggtaaac gcgtagtgct gtttgggcca tttgccacct tctcaaccaa attcctcaaa
    11401 aacggttcat tgtgggatct gatgagttcc accaccacct ctaacgtgat gccgatgatg
    11461 cacgtcgggc tggataattt cgatctggtg aaatatctgg tgagtcaggt gatgttgagt
    11521 gaagaggatc gttttgaagc gttgaaagag tactatccgc aggcaaaaaa agaggactgg
    11581 cgtttgtggc aagcggggca gcgcgtgcag attatcaagc gtgatgccga gaaaggtggc
    11641 gtactgcgtc tgggtactga agtggtcagt gaccagcaag ggaccattgc cgcgctgctg
    11701 ggggcatcgc caggggcgtc aaccgccgcg ccgattatgt tggatctgct ggaaaaagta
    11761 tttggcgatc gcgtttccag cccgcaatgg caggctacgt tgaaagcgat cgttccgtct
    11821 tatggacgca agctgaacgg tgatgtagcg gcaacagaac gcgagttgca gtacaccagc
    11881 gaagtgctgg ggctgaaata cgacaagccg caggcagcag atagtacgcc aaaaccgcag
    11941 ttgaaaccgc aacccgttca aaaagaagtg gcggatattg cgttgtaatg atgtgccaca
    12001 tccggcatgg tatgccggat gtggcgtatg ctgataagac gcgccagcgt cgcatcaggc
    12061 accggctcgg gcgttagatg tcggatgcgg cgtgaccgcc ttattcggcc tacggggtgc
    12121 cgcacttcac agcattttag cgaactaccg cgttgtcaat tttctcttcc gccttccaga
    12181 cgcggtactt cacatctaca ttgtctggcg tatacaccac gatcggcagc ttgctgttgt
    12241 aacgcagcat tccagcatcg cccagatacg cggtgacaaa tttcttctct ttcttgccat
    12301 ccgggcaggc catcatcgtc gaaaccgggg aactgacttt atcaaagaca taataatcat
    12361 agccccagcc ttccagcgtt ttgttttcca gcttcccgcc gagacgatgc aaattgcaat
    12421 cgacttccag cgtctgaccg attaacagtt ctactttcag ggtagattca tcttcttgcg
    12481 gggttaactg aatcacctga cgcttcatcc ctttttcagc ttgtggataa ggcgcgattt
    12541 tttccagtgg ctggacgctt tctgccgccc aggcggaagt ggtagcgaaa gcggcaaaca
    12601 atactgcagg tagaatggtc ttcatttttt tgctttcctt cttcatttat aggttttcac
    12661 cgatgatgtc gctgaaggtt aacatattca tcagatttat taatttgctt tatgaataac
    12721 ttaaagaaca ttcagacaat tttacctgct cttttagtgt taaattctaa tgagagagca
    12781 aaccagcaga
//
LOCUS       DAZFVO020000140        12560 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFVO020000140 DAZFVO020000000
VERSION     DAZFVO020000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.141"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            143..478
                     /locus_tag="ACHKNB_RS19140"
                     /old_locus_tag="pgaptmp_003828"
     CDS             143..478
                     /locus_tag="ACHKNB_RS19140"
                     /old_locus_tag="pgaptmp_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGU4197254.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            638..1081
                     /gene="yjdN"
                     /locus_tag="ACHKNB_RS19145"
                     /old_locus_tag="pgaptmp_003829"
     CDS             638..1081
                     /gene="yjdN"
                     /locus_tag="ACHKNB_RS19145"
                     /old_locus_tag="pgaptmp_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HGU4197255.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1214..2002
                     /gene="phnC"
                     /locus_tag="ACHKNB_RS19150"
                     /old_locus_tag="pgaptmp_003830"
     CDS             1214..2002
                     /gene="phnC"
                     /locus_tag="ACHKNB_RS19150"
                     /old_locus_tag="pgaptmp_003830"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HGU4197256.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2027..3043
                     /gene="phnD"
                     /locus_tag="ACHKNB_RS19155"
                     /old_locus_tag="pgaptmp_003831"
     CDS             2027..3043
                     /gene="phnD"
                     /locus_tag="ACHKNB_RS19155"
                     /old_locus_tag="pgaptmp_003831"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4197257.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3149..3928
                     /gene="phnE"
                     /locus_tag="ACHKNB_RS19160"
                     /old_locus_tag="pgaptmp_003832"
     CDS             3149..3928
                     /gene="phnE"
                     /locus_tag="ACHKNB_RS19160"
                     /old_locus_tag="pgaptmp_003832"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HGU4197258.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3949..4674
                     /gene="phnF"
                     /locus_tag="ACHKNB_RS19165"
                     /old_locus_tag="pgaptmp_003833"
     CDS             3949..4674
                     /gene="phnF"
                     /locus_tag="ACHKNB_RS19165"
                     /old_locus_tag="pgaptmp_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HGU4197259.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4675..5127
                     /gene="phnG"
                     /locus_tag="ACHKNB_RS19170"
                     /old_locus_tag="pgaptmp_003834"
     CDS             4675..5127
                     /gene="phnG"
                     /locus_tag="ACHKNB_RS19170"
                     /old_locus_tag="pgaptmp_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HGU4197260.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETVIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5124..5708
                     /gene="phnH"
                     /locus_tag="ACHKNB_RS19175"
                     /old_locus_tag="pgaptmp_003835"
     CDS             5124..5708
                     /gene="phnH"
                     /locus_tag="ACHKNB_RS19175"
                     /old_locus_tag="pgaptmp_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HGU4197261.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5708..6772
                     /gene="phnI"
                     /locus_tag="ACHKNB_RS19180"
                     /old_locus_tag="pgaptmp_003836"
     CDS             5708..6772
                     /gene="phnI"
                     /locus_tag="ACHKNB_RS19180"
                     /old_locus_tag="pgaptmp_003836"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HGU4197262.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6765..7610
                     /gene="phnJ"
                     /locus_tag="ACHKNB_RS19185"
                     /old_locus_tag="pgaptmp_003837"
     CDS             6765..7610
                     /gene="phnJ"
                     /locus_tag="ACHKNB_RS19185"
                     /old_locus_tag="pgaptmp_003837"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HGU4197263.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7607..8365
                     /gene="phnK"
                     /locus_tag="ACHKNB_RS19190"
                     /old_locus_tag="pgaptmp_003838"
     CDS             7607..8365
                     /gene="phnK"
                     /locus_tag="ACHKNB_RS19190"
                     /old_locus_tag="pgaptmp_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HGU4197264.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8542..9222
                     /gene="phnL"
                     /locus_tag="ACHKNB_RS19195"
                     /old_locus_tag="pgaptmp_003839"
     CDS             8542..9222
                     /gene="phnL"
                     /locus_tag="ACHKNB_RS19195"
                     /old_locus_tag="pgaptmp_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HGU4197265.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9219..10355
                     /gene="phnM"
                     /locus_tag="ACHKNB_RS19200"
                     /old_locus_tag="pgaptmp_003840"
     CDS             9219..10355
                     /gene="phnM"
                     /locus_tag="ACHKNB_RS19200"
                     /old_locus_tag="pgaptmp_003840"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HGU4197266.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10355..10912
                     /gene="phnN"
                     /locus_tag="ACHKNB_RS19205"
                     /old_locus_tag="pgaptmp_003841"
     CDS             10355..10912
                     /gene="phnN"
                     /locus_tag="ACHKNB_RS19205"
                     /old_locus_tag="pgaptmp_003841"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HGU4197267.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10899..11333
                     /gene="phnO"
                     /locus_tag="ACHKNB_RS19210"
                     /old_locus_tag="pgaptmp_003842"
     CDS             10899..11333
                     /gene="phnO"
                     /locus_tag="ACHKNB_RS19210"
                     /old_locus_tag="pgaptmp_003842"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HGU4197268.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11335..12093
                     /gene="phnP"
                     /locus_tag="ACHKNB_RS19215"
                     /old_locus_tag="pgaptmp_003843"
     CDS             11335..12093
                     /gene="phnP"
                     /locus_tag="ACHKNB_RS19215"
                     /old_locus_tag="pgaptmp_003843"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HGU4197269.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSSQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12240..>12560)
                     /gene="yjdP"
                     /locus_tag="ACHKNB_RS19220"
                     /old_locus_tag="pgaptmp_003844"
     CDS             complement(12240..>12560)
                     /gene="yjdP"
                     /locus_tag="ACHKNB_RS19220"
                     /old_locus_tag="pgaptmp_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HGU4197270.1"
                     /translation="FPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDRGRD
                     YEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERRMED
                     EYGR"
BASE COUNT         2713 a         3814 c         3582 g         2451 t
ORIGIN      
        1 tttcctttca aatgaaaacg atcgtcgtct aaaatcagca gtacccccga caaactcagg
       61 gattttgtgt ataattgcgg cctttttcgg caatctgccg ttttttggcg cttttgccct
      121 gctgactttt gaggaaatcc acatgtcatt accacactgc ccaaaatgca actccgaata
      181 cacttacgaa gataacggca tgtacatctg cccggaatgt gcctacgaat ggaacgacgc
      241 agaacctgca caggaaagcg acgagctgat cgttaaagat gctaacggca atctgctggc
      301 tgacggcgac agcgttacca tcattaaaga tctgaaggtg aaaggtagct cttcgatgct
      361 gaaaattggc accaaagtga aaaacatccg cctggttgaa ggcgaccata acatcgattg
      421 caaaatcgac ggttttggtc cgatgaaact gaaatctgag tttgtgaaaa agaactgatt
      481 gtattgtgat cggtaagccg gataaggcgc tcgcgccgca tccggcaacg atgccgactg
      541 cctgatgcga cgctgaccgc gtcttatcag gcttctctct catctgtata aatttcgaac
      601 tacacttaac tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc
      661 ttttgccggt aactgtgccg acgcaattgc ttattatcaa cgtacgttgg gcgctgaact
      721 gctctataaa atcagcttcg gcgaaatgcc aaaatcagcg caggacagcg ccgagaactg
      781 cccttccgga atgcaatttc ccgataccgc catcgcccac gccaacgtgc gcattgccgg
      841 aagcgacatc atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac
      901 gctggtgctc gattcgcaac aggtcgaaga aggaaaacgc tggtttgaca atcttgccgc
      961 taacggaaaa atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt
     1021 caccgataaa tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata
     1081 acccgccggg aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt
     1141 tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta
     1201 gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca
     1261 gcatcaggcg ctgcatgcgg ttgatctgaa cattcatcac ggtgaaatgg tggctctgct
     1321 tgggccgtcg ggttccggca aatccaccct tttacgtcac ttaagcggtt tgattaccgg
     1381 cgataaatcc gccggcagcc atatcgagct gctgggccgc acagtccagc gcgaaggccg
     1441 tctggcgcgc gatatccgca aaagccgcgc caacaccggc tacatcttcc aacaattcaa
     1501 cctggtgaac cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc
     1561 gttctggcgc acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc
     1621 gctgacccgc gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg
     1681 acagcagcag cgtgtggcga ttgcccgcgc gctgatgcag caggcgaagg tgattctggc
     1741 cgatgaaccc atcgcctcgc tggacccgga atccgcccgc atcgtgatgg acaccctgcg
     1801 cgacatcaat cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc
     1861 cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg cacgttttct acgacggcag
     1921 cagccaacag tttgataacg aacgttttga ccatctctac cgcagcatta atcgcatcga
     1981 agagaacgcg aaagctgcct gacatcccca tcattgagga aaacgaatga acgctaagat
     2041 aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa gcccggcaca
     2101 cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa
     2161 cctgaaaccg caatggacgc cattcttaca ggatatggag aagaagctgg gcgtgaaggt
     2221 gaacgccttc tttgccccag actacgcagg cattatccag ggaatgcgct tcaataaagt
     2281 ggatatcgcc tggtacggca acctgtcggc aatggaagcg gtggatcgcg ccaacggcca
     2341 ggtcttcgcc cagacggtcg cggcggatgg atcgccaggt tactggagcg tgttgatcgt
     2401 caacaaagat agtccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac
     2461 cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt
     2521 tgccaaaaac aatatctccg ccagcgactt taagcgcacc gtcaacgctg ggcatgaaac
     2581 caacgcgctg gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct
     2641 cgacaagctg aaaacctccg cgccagagaa gctgaaagaa ctgaaggtga tctggaagtc
     2701 gccgctgatc ccaggcgatc cgatcgtctg gcgtaaaaac ctttccgaaa ccaccaaaga
     2761 caagatctac gacttcttta tgaactacgg caaaacgccg gaagaaaaag cggtgctgga
     2821 acgcctgggc tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca
     2881 gctcgcgctg tttaaagaga tgcagggcgt gaaaagcaat aaaggactga atgagcagga
     2941 caagctggca aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa
     3001 cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag
     3061 acgccccggc gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc
     3121 ttatccggcc tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg
     3181 tagctggttc tcgcttctga gctgggccgt tgttctcgcc gtgttggtcg tctcgtggca
     3241 gggcgcggaa atggccccgc tcacgctgat taaagacggc ggcaacatgg cgacgttcgc
     3301 cgccgacttc ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt
     3361 cacgctgcaa atcgccgtct ggggcacggc gctggcggtg gttctctcca tccccttcgg
     3421 cctgatgagc gccgaaaacc tggtgccgtg gtgggtttac cagcccgttc gccgcctgat
     3481 ggacgcctgc cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg
     3541 cctcggcccg ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa
     3601 actgctttcc gaagcggtgg aagcgattga acccggcccg gtggaaggca ttcgcgccac
     3661 cggcgccaac aagctcgaag agatcctcta cggcgtgctg ccgcaggtga tgccgctgct
     3721 gatctcctac tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat
     3781 ggtcggtgca ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca
     3841 acaaacctgc gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc
     3901 tcaacggttg cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct
     3961 acacatccga ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag
     4021 cttcgtcaac actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc
     4081 tttgaggtga accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg
     4141 gtacagcgcc gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc
     4201 aacgcccagg cgcgttttag ccagaacctg ctggaccagg gtagccatcc caccagcgaa
     4261 aaactgctgt cggtattgcg ccccgcgtcc ggtcacgtcg ctgacgcgct ggggattacc
     4321 gagggcgaga acgttatcca tttgcgcacc ctgcgtcgcg tcaacggcgt cgcgctctgt
     4381 ttaatcgacc actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc
     4441 ggttcgctgc acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg
     4501 cggatcagcg cccgtcgcgc ccaggccaaa gagtgccagc gtcttgaaat tccgaatatg
     4561 tcgccgctgc tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag
     4621 tactccgtca gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac
     4681 gcagataccg cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa
     4741 ctggcagccc gcctgaaagc actgaacatc accgccgact atgaggttat ccgcgccgcc
     4801 gaaaccggcc tggtgcagat tcaggcgcgg atgggcggca ccggcgaacg cttttttgcc
     4861 ggcgacgcca cgctcacccg cgccgccgta cgcctgactg acggcacgct cggctacagc
     4921 tgggtgctgg ggcgcgacaa acagcacgcc gaacgctgcg cgctgattga cgcgctgatg
     4981 cagcaatctc gctattttca aaacttatca gaaaccgtta ttgccccgct ggacgctgac
     5041 cgtatggcac gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt
     5101 acgatggttc gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg
     5161 atgcccagca cagttttcgt cgcctgttaa aggcgatgag cgagccgggc gtgattgtcg
     5221 ccctgcatca gctcaaacgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc
     5281 tgacgctggc cgataacgac acgccggtgt ggcttgccac gccattaagt aacgatatcg
     5341 tcaatcagag cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga
     5401 ccttcgcggt gacggatgag gcgatttcca gcgaacagct caacgccctt tccaccggca
     5461 ccgccgttgc gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg
     5521 gacgcatgtt gcgtctcacc ggcgcgggca ttgccgaaga acgaatgatc gctccgcagc
     5581 tgccggagtg cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg
     5641 acctgatcct gacctgcggc gagcgcctgc tggctgttcc gcgaaccact catgtggagg
     5701 tgtgctgatg tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct
     5761 gcaagagagc cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccagattga
     5821 acagcagctt aacctcgcgg tagatcgcgt gatgaccgaa ggcggcattg ccgaccgcga
     5881 actggcggcg ctggcgctga aacaggccag cggcgataac gtcgaagcga ttttcctgct
     5941 acgcgcctac cgcaccacgc tggcgaagct ggcggtaagc gagccgctcg acaccacgga
     6001 gatgcgtctc gaacggcgta tttcagcggt ttataaagat attcccggcg gtcagttgct
     6061 tggcccaacc tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggcga
     6121 agcgccgacg ctgaccaccg cagacagcga acaatccccg tcgccgcacg ttttcagcct
     6181 gctggcgcgt caggggctgg cgaagtttga agaggatagc ggcgcacagc cggatgacat
     6241 cacccgcacg ccgccggttt acccctgttc acgttcttcc cgtttgcagc agttgatgcg
     6301 cggcgacgaa ggctatttgc tggcgctggc ctactctacc cagcgcggtt acggacgcaa
     6361 tcacccgttc gcgggcgaaa tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga
     6421 gctgggattt gcggtgaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg
     6481 ttttattgac ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtgttcgg
     6541 catgagcgag cgcaaagcga tggcgatggc gctggttgac cgtgcgttgc aggctccgga
     6601 gtacggcgag cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga
     6661 caacgtcgaa gccgcaggct ttgtctcgca cctcaaactc ccgcactacg tcgatttcca
     6721 ggccgaactg gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg
     6781 gctacaactt tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa
     6841 aagcggtggc gatccccggt tatcaggtgc cgtttggcgg gcgcgagatg cccatgccat
     6901 acggctgggg aaccggcggc atacagctca ccgccagcgt gattggcgaa agcgacgtgt
     6961 tgaaggtgat tgaccagggc gcggatgaca ccaccaacgc cgtgtcgatt cgcaacttct
     7021 ttaagcgcgt gaccggggtg aacaccactg aacgtacgga tgatgcgacg cttatccaga
     7081 cgcgccaccg cattcccgaa acgccgctga cggaagatca gatcattatc ttccaggtgc
     7141 cgatccccga accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc accatgcacg
     7201 cgctggaaga gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc
     7261 atatcgccac tacctacgcc tatccggtga aggtgaacgg gcgctacgta atggacccgt
     7321 cgccgatccc gaaattcgat aacccaaaaa tggacatgat gcccgccctg caactgttcg
     7381 gcgcggggcg cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg
     7441 atttcgacga tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat
     7501 cgacccacag ctatcttgat gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct
     7561 gctccgatac cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt
     7621 actttcggtc aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc
     7681 ttttgattta tggccggggg aagtgctggg cattgtcggg gagtccggct ccgggaagac
     7741 cacgctgctg aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcactatga
     7801 gagccgttcg ctgtacgcaa tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg
     7861 gggcgtagtg catcagcatc cgctcgacgg cctgcgccgc caggtgtcgg caggcggcaa
     7921 tatcggcgag cggctgatgg cgaccggggc acgtcattac ggcgatattc gtgccaccgc
     7981 gcagaagtgg ctggaagagg tggaaattcc cgccaaccgg atcgacgacc tgccgaccac
     8041 cttttccggc ggcatgcagc aacgtttgca gatcgcccgc aacctggtga cgcatccgaa
     8101 gctggtgttt atggacgagc cgaccggcgg actggatgtg tcggtacagg cacgcctgct
     8161 cgacctgctg cgcggcctgg tggtggagct gaacctcgcg gtagtgattg tcacccacga
     8221 tttaggcgtc gcccgcctgc tggcggaccg tttgctggtg atgaagcagg ggcaagtggt
     8281 ggagagtggg ttaaccgacc gcgtgctcga cgacccgcac catccgtata cacaactgct
     8341 ggtgtcatcg gttttgcaga attgaatgca acaccgtgcg cgctgatccc ctcaccccgg
     8401 ccctctcccg gagggagagg gagaaaaccg gcgcaatatt caacaccgcg cacaatcggt
     8461 cccctcgccc cttcggggag agggttaggg tgaggggcaa tgcgcgcacc gctccccaat
     8521 atgcgaacca cgaggcgaaa aatgattaac gtacaaaacg tcagtaaaac cttcatcctg
     8581 caccagcaaa acggcgtgcg cctgcccgtc ttacaacgcg cctcgctcac cgtcaacgca
     8641 ggcgaatgcg tggtgctcca cggtcactcc ggcagcggca aatcaaccct gctgcgctcg
     8701 ctgtacgcca actatctacc cgacgaaggt caaatccaga tcaaacacgg tgacgagtgg
     8761 gtagacctag tcaccgcgcc agcgcgcaaa gtggtggaga tccgcaaaac taccatcggc
     8821 tgggtgagcc agtttctgcg cgtcatcccg cgtatctcag cactggaagt ggtgatgcag
     8881 ccgctgctcg atacaggcgt tccgcgtgaa gcctgcgccg ctaaagccgc gcgtcttctc
     8941 acccgcctga acgtgccgga acgcctgtgg cacctggcac catcgacctt ctccggcggc
     9001 gaacagcagc gcgtcaacat cgcccgcggc tttatcgtcg actaccccat tctgctgctc
     9061 gacgaaccta ccgcctcgct ggacgccaaa aacagcgccg cggtggtgga actgattcac
     9121 gaagccaaag cccgtggcgc agccatcgtc ggcatcttcc atgacgaagc tgtacgtaat
     9181 gacgtcgccg accgcctgca cccaatggga gcctcttcat gattatcaat aacgttaagc
     9241 tggtgctgga aaacgaggtg gtgcacggtt cgctggaggt gcaggatggc gaaatccgcg
     9301 cctttgccga aagccagagc cgcctgccag aagcgatgga cggcgaaggt ggctggctac
     9361 tacccggcct gattgagctg cataccgaca acctcgataa attcttcacc ccgcgcccga
     9421 aagtcgactg gcccgcccat tcggcgatga gcagccacga cgcgctgatg gtggcaagcg
     9481 gcatcaccac cgtgctggac gcggtggcga ttggcgacgt gcgcgacggc ggcgatcggc
     9541 tggagaatct ggagaagatg atcaacacca tcgaagagac gcagaaacgc ggcgtcaacc
     9601 gcgccgagca ccgcctgcac ctgcgctgcg aactgccgca tcacaccaca ctgccgctgt
     9661 ttgaaaaact ggttcagcgc gaaccggtga cgctggtgtc gctgatggac cactcaccgg
     9721 gccagcgcca gttcgctaac cgcgagaagt atcgcgaata ttatcagggc aaatactccc
     9781 tcaccgatgc gcagatgcag cagtacgaag aagagcaatt ggcgctcgcc gcacgctggt
     9841 cgcagccgaa tcgcgaatcc atcgccgccc tgtgccgcgc gcgacaaatt gcgctcgcca
     9901 gccacgatga cgccacgcac gcccacgttg ccgaatccca ccaacttggc agcgtgatcg
     9961 ccgaatttcc caccacgttc gaagcagcgg aagcctcgcg taagcatggc atgaacgtgc
    10021 tgatgggtgc gccaaatatc gtgcgtggcg gctcgcactc cggcaatgtg gcggccagtg
    10081 aactggcgca gcttggtctg ctggatatcc tctcttccga ctactacccc gccagcctgc
    10141 tggatgcggc gttccgcgtc gccgatgacg agagcaaccg ctttacgcta ccgcaggcgg
    10201 tgaggctggt gaccaaaaat ccggcgcagg cgctgaatct tcaggatcgc ggggtgattg
    10261 gtgagggtaa acgcgctgac ctggtgctgg cgcatcgcca gggcaatcac attcatatcg
    10321 accacgtctg gcgtcagggt aaaagggtgt tctgatgatg ggaaaactga tttggttaat
    10381 ggggccgtcc ggctccggga aagacagcct gctggcggaa ctccgcctgc gggaacaaac
    10441 gcagttactg gtggcgcatc gctacatcac gcgcgacgcc agcgccggaa gtgaaaacca
    10501 tatcgccctg agtgagcggg agttttttac ccgcgcgggg caaaacctgc tggccttaag
    10561 ctggcacgct aacggtctgt attatggcgt cggcatcgag attgacctct ggctgcacgc
    10621 tggattcgac gtagtcgtca acggttcacg cgcgcatttg ccgcaggcac gggcgcgcta
    10681 tcaatcggcg ctgctgcccg tctgtttaca ggtttcgccg gagatcctgc gccagcgcct
    10741 ggaaaaccgt ggccgtgaaa acgccagtga aattaacgcc cgcctggcgc gcgccgcccg
    10801 ctatactcca caggattgcc atacgctcaa caatgacggc agcctgcgcc agtcggtcga
    10861 cacgctgctg acgctgatcc atcagaagga gaaacaccat gcctgcttgt gagcttcgcc
    10921 cggccacgca gtacgatacc gacgcggttt acgcgctgat ttgtgagcta aaacaggcgg
    10981 agtttgacca ccatgcgttt cgcgtgggct ttaacgccaa cctgcgcgac ccaaacatgc
    11041 gctaccatct ggcgctgctg gatggcgaag ttgtcggcat gatcggcctg catttgcagt
    11101 ttcatctgca tcatgtcaac tggatcggcg aaattcagga actggtggtg atgccgcagg
    11161 cgcgcggtct gaacgtcggc agtaaattac tggcatgggc agaagaagaa gcccgccagg
    11221 ccggggccga aatgaccgag ctttccacca acgtgaagcg ccacgacgca caccgttttt
    11281 acctgcgtga aggctacgag cagagccact tccgcttcac caaggcgctg taacatgagc
    11341 ctgaccctca cgctcaccgg cactggcggc gcacagggcg ttccggcatg gggctgcgag
    11401 tgcgcggcct gcgccagagc gcggcgctcg ccgcagtatc gccgccagcc atgcagcggc
    11461 gtagtgaagt ttaacgacgc gatcaccctg atcgacgccg ggcggcacga tctcaccgat
    11521 cgctggtcgc ctggatcgtt ccagcagttt ttgctgacgc actaccatat ggatcacgtc
    11581 caagggctat ttccgctacg ctggggcgtg ggcgatgtga tcccggtcta cggcccgccg
    11641 gatgaacagg gctgcgacga tctgtttaaa catccgggcc tgcttgattt cagccacaca
    11701 gtggaaccgt ttgtggtgtt tgatttgcag gggttgcagg tcacgccgct gccgcttaac
    11761 cactcaaaac tgaccttcgg ttatctgctg gaaacggcac atagccgggt ggcgtggctg
    11821 tctgacaccg ctggcctgcc ggaaaaaacg ctgaaatttt tactcaataa tcatccgcag
    11881 gtgatggtga ttgattgcag tcacccgccg cgcgcggatg caccgcgtaa tcactgtgat
    11941 ttaaataccg tgcttgcgct gaatcaggtt atccgctcgt cacaggtgat tctgacccat
    12001 atcagccacc agtttgatgc gtggctgatg gaaaacgcac taccgtcagg gtttgaggtg
    12061 gggtttgatg ggatggagat tggggtggcg tgatgagagg gaatgtgcgc gctggccccc
    12121 tcaccctaac cctctcccca gaggggcgag gggaccgatt gtgctcaata ttgaatattg
    12181 cgctcgtttt ctccctctcc ccattggggt gaggggcgat gcctgctcca tacccaacct
    12241 catcgcccat actcatcttc cattctccgc tcttcatcct ccagttgccg acgctcctga
    12301 tcaagctggc gctggcgatc gtccagctgc ctgcggcgat cttcaaactg gcggcggcgg
    12361 tcgtcatatt gtctgcgccg atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa
    12421 tcttcatagt ctcgcccacg gtcagggtta taagcgtcat taatcgcctg ctgaatatta
    12481 ccaatagtat cgtcgataat atcagcctgg gccggaacgg tggacagcgt gagcagggtg
    12541 aataaaagaa atagcggaaa
//
LOCUS       DAZFVO020000141        12520 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFVO020000141 DAZFVO020000000
VERSION     DAZFVO020000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.142"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(2..544)
                     /gene="ecpR"
                     /locus_tag="ACHKNB_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /gene_synonym="matA"
     CDS             complement(2..544)
                     /gene="ecpR"
                     /locus_tag="ACHKNB_RS19225"
                     /old_locus_tag="pgaptmp_003845"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HGU4197271.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            1362..1595
                     /gene="ykgL"
                     /locus_tag="ACHKNB_RS19230"
                     /old_locus_tag="pgaptmp_003846"
     CDS             1362..1595
                     /gene="ykgL"
                     /locus_tag="ACHKNB_RS19230"
                     /old_locus_tag="pgaptmp_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HGU4197272.1"
                     /translation="MKTQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSER
                     NVRQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(1630..1770)
                     /gene="ykgO"
                     /locus_tag="ACHKNB_RS19235"
                     /old_locus_tag="pgaptmp_003847"
     CDS             complement(1630..1770)
                     /gene="ykgO"
                     /locus_tag="ACHKNB_RS19235"
                     /old_locus_tag="pgaptmp_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HGU4197273.1"
                     /translation="MKVLNSLRTAKERHQDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1770..2033)
                     /gene="ykgM"
                     /locus_tag="ACHKNB_RS19240"
                     /old_locus_tag="pgaptmp_003848"
     CDS             complement(1770..2033)
                     /gene="ykgM"
                     /locus_tag="ACHKNB_RS19240"
                     /old_locus_tag="pgaptmp_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGU4197274.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(2396..2497)
                     /gene="ykgR"
                     /locus_tag="ACHKNB_RS19245"
                     /old_locus_tag="pgaptmp_003849"
     CDS             complement(2396..2497)
                     /gene="ykgR"
                     /locus_tag="ACHKNB_RS19245"
                     /old_locus_tag="pgaptmp_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HGU4197275.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2971..3060)
                     /locus_tag="ACHKNB_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /pseudo
     CDS             complement(2971..3060)
                     /locus_tag="ACHKNB_RS19250"
                     /old_locus_tag="pgaptmp_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3613..7866
                     /gene="fdeC"
                     /locus_tag="ACHKNB_RS19255"
                     /old_locus_tag="pgaptmp_003851"
     CDS             3613..7866
                     /gene="fdeC"
                     /locus_tag="ACHKNB_RS19255"
                     /old_locus_tag="pgaptmp_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HGU4197276.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLAEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISNIE
                     VIGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTIVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLAVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKIIRTFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
     gene            complement(7988..8845)
                     /locus_tag="ACHKNB_RS19260"
                     /old_locus_tag="pgaptmp_003852"
     CDS             complement(7988..8845)
                     /locus_tag="ACHKNB_RS19260"
                     /old_locus_tag="pgaptmp_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4197277.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRYEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            9094..9960
                     /locus_tag="ACHKNB_RS19265"
                     /old_locus_tag="pgaptmp_003853"
     CDS             9094..9960
                     /locus_tag="ACHKNB_RS19265"
                     /old_locus_tag="pgaptmp_003853"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGU4197278.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKNS"
     gene            complement(10124..10717)
                     /gene="rclC"
                     /locus_tag="ACHKNB_RS19270"
                     /old_locus_tag="pgaptmp_003854"
     CDS             complement(10124..10717)
                     /gene="rclC"
                     /locus_tag="ACHKNB_RS19270"
                     /old_locus_tag="pgaptmp_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HGU4197279.1"
                     /translation="MEKYLHMLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(10729..10965)
                     /gene="rclB"
                     /locus_tag="ACHKNB_RS19275"
                     /old_locus_tag="pgaptmp_003855"
     CDS             complement(10729..10965)
                     /gene="rclB"
                     /locus_tag="ACHKNB_RS19275"
                     /old_locus_tag="pgaptmp_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HGU4197280.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(11074..12399)
                     /gene="rclA"
                     /locus_tag="ACHKNB_RS19280"
                     /old_locus_tag="pgaptmp_003856"
     CDS             complement(11074..12399)
                     /gene="rclA"
                     /locus_tag="ACHKNB_RS19280"
                     /old_locus_tag="pgaptmp_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HGU4197281.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3502 a         2694 c         2934 g         3390 t
ORIGIN      
        1 tttactgaac caacttatat atttttgagt acagcttggc ctcagcatta cgccgatggg
       61 tatacactgt cttcacacta caattttcaa ttctggcaat cgatttaggt tgcattccct
      121 gggccgtcat gcggataatt tccatttccc ggtcagtgat tttatctttc ttaatatctt
      181 ttctcaggaa cctgccatta atcacatagg ccagttcttt tctaatatca cgactcagtc
      241 ctgcgtatac cacgccccta atattgctgt tgtaatacca ataatttgct aaggcttctg
      301 actttctggc cgcaatcaat acgacctgtt tcccctttac cgcggataac cattcattat
      361 cacggtttat aaattcagat aaagaatccg tattgagatt gataaacacg aaatcttttc
      421 taattttctc caacgataga taaattaatc tgtcgatatc cacaatcagt tcagatagtc
      481 ctttatagaa gtaggcgtca tggggagacc agatgtattt atcagaacgg ttttgacatt
      541 ccatatggtt tttaacctca tagtccctgc tgtaatcact ttgccatgtc actactttcc
      601 aaacctgtaa tttttacagg tgcattcagt ttccttagta aaccggaagt aaataagata
      661 cgtagtacat atcaataaag gttaaacgag attaagttta attattttcg attgggctta
      721 aatttatgtt tttttcccga tgagtgtcaa tatgtgaacg gaaaaggaat tcaatggtta
      781 agtcaatatt tagaaaaact gaaaatattg cttttttgat aagaagaaaa agacatttgg
      841 tttcataagg ttattggcaa tataacgttc aattcttact tgcattttat attggtcagg
      901 aactttgccc tgaagtgtta ggaatagtct taatgcgctg tgtgatggac tagtaaaaaa
      961 acaagtttta agggtaaggg atagaatata aaactataat gagctgtcat tgaagatgat
     1021 ttgccaatca gtaagaaatt ttaatggatt aacatgccgc acaaaatggt tataggtgtt
     1081 cattacaata agtgatggaa aattgaacgg gcgtatgtac gtcatctgtt cggtaagacc
     1141 cccaagagcc agattaagtg aagttaaacg aaggctgcca gctgtcacag gaacgatggc
     1201 ctctttgtca gggcactgag tgcagttatc tgtgcgcgtt actctaactg gcactcgaac
     1261 gtcggcgatg ggcttaccgg tggatcaaac tgttcctgtg gcgtaaacgt ttttatgtta
     1321 gtaccaacag atttaccaca tttatcatct taacagtttg agtgaaaacg caggctgcgc
     1381 cgcaaaggga ggagactgag tggcgtgttc agtctaaaag agggttgatg ccagcataca
     1441 ggggcgaagc tggtcaacaa gtcaacatta agataatgga atattcggag agaaacgtca
     1501 ggcagttggc ctcaaatgaa caggaagaat atatccccag gaaaattaat gttggtgtta
     1561 taaacacacc aacattaatc cgatctgatt attaatcaga caaatatcgt taattcgtcg
     1621 aattttgaat caacgttttt tcttacgacc ctgaacggcc ttaaaacgtg gattagattt
     1681 acaaatcaca tacaaccgtc cttttcgctt cacaatctga cagtcttgat ggcgttcttt
     1741 tgcggtacgc agagagttaa gaactttcat tacgccccct ttttcgtgct aacaaaacga
     1801 ccaaaacgtt gggtgaaccg tgcaacattt ccttctgatg ccactgttct cagcttccct
     1861 gtatagaacg ggtgcgattt cgaagagaca tcaattgtta cgtacggata cgttacgcca
     1921 tccagctcaa tctcacggtc tgttttgata gtcgagccga ttttaaagta ctcatcaaca
     1981 ctggtgtcgt ggaacaccac agtacgatac tcaggatgga tattgggctt catcattttt
     2041 acctgttatg ttataacata accataaaga tacatgctca tccggatcat ttcaacaccc
     2101 aatccctgtg actctttgtg gatgtggaat tttcgcaacg gggaactctg gcattgttag
     2161 cagccattgg tgacgctccc ataaacgcct ggatgagtga ttgatgatgg ttttgctgag
     2221 agtatggatt tttcgacaat ggaggttatc tccactttac tggccgatga tttatcgcag
     2281 tatgattgat tacctgacag cgtgtattcc tctcccacgg atataataat tacttacaaa
     2341 aagggagagg atgcatattt taaatatcac tgaagtgaac agtttattgc cgttattaat
     2401 agaaatggag aaataaatag gcgtattcta caattgcgac aaaaacaacg atattaatca
     2461 gtttatgact gatttgttgt actttattct ctttcattgg tacttcctcg ctttaaaaaa
     2521 gagcgcactt cgtaagtgcc cttatataaa taacgagttt ggtcaaccaa ttttttgaca
     2581 tgtatcacaa atttgaatag atgtattaca tcggctatct tttattgcac caacgtcatt
     2641 gatatatgtc gcctgaagtc agttccggga atgagtctga tctcaagact ggcccagtcc
     2701 gggcgttgat tggcgctgag gagcatatcg catctcatca taatgtcgta tctcctgggg
     2761 tgttatacaa gatatcgttg ttggtgacct gggagaggaa ttgagttcta ttaaaccgtc
     2821 aactatgccg gatacatact ggattacact gcaggcacgc cttacgagag aacgtgccgc
     2881 agtgacgggt taattatctg aaagaatttg tgaggctgta tcggttactc attgatttga
     2941 tagttttact ctcgggagag taatagatat ttaatctatt aacggaaacc agccagttcc
     3001 tttcgatgcc tgaatttgat tccatagttt atccggaatg gttaattcat gaataagttg
     3061 tgttctcgtt catgagaagc ataacgtaaa gggaaaagct cgattagacg gcaggatttg
     3121 tcaggggtta tgaacgaaat tcataaatct gtttgagtgt tgcgatgggt agtgcaagtt
     3181 cgaaatctcc gcaatttaca gtccgatgaa ggaaaatgaa tatccataaa aaatatatcg
     3241 atttattctg gcgtatatac ctacttcgac gtatttccaa tagttgttaa ttaaaggcag
     3301 gccattatta ttcactctga ataatgaatt attcactgtc cgcagagtaa aaaatataac
     3361 ttaggtgtct atttaatgac ttgcacaaaa agctaaattt tcacccataa ataaaaatat
     3421 aatcccgcgc ccaaccacct gatgagtggc tacagacact ggatatatta ggtggcggtg
     3481 cactttctta cataaaggta tttccttttc tgcggaaaag gaaatcggga aatccccggt
     3541 ttttctgaca agcagacgcc attatttgtg tctgcctatg ttcgttaatt cgttcatcag
     3601 gaaattatct caatgtcgcg ttataaaaca ggtcataaac aaccacgatt tcgttattca
     3661 gttctggccc gctgcgtggc gtgggcaaat atctctgttc aggttctttt tccactcgct
     3721 gtcaccttta ccccagtaat ggcggcacgt gcgcagcatg cggttcagcc acggttgagc
     3781 atgggaaata ctacggtaac tgctgataat aacgtggaga aaaatgtcgc gtcgtttgcc
     3841 gcaaatgccg ggacattttt aagcagtcag ccagatagcg atgcgacacg taactttatt
     3901 accggaatgg ccacagctaa agctaaccag gaaatacagg agtggctcgg gaaatatggt
     3961 actgcgcgcg tcaaactgaa tgtcgataaa gatttctcgc tgaaggattc ttcgctggaa
     4021 atgctttatc cgatttatga tacgccaaca aatatgttgt tcactcaggg agcaatacat
     4081 cgtaccgacg atcgtactca gtcaaatatt ggttttggct ggcgtcattt ttcaggaaat
     4141 gactggatgg cgggggtgaa cacctttatc gaccatgatt tatcccgtag tcatacccgc
     4201 attggtgttg gtgcggaata ctggcgcgat tatctgaaac tgagcgccaa tggttatatt
     4261 cgggcttctg gctggaaaaa atcgccggat attgaggatt atcaggaacg cccggcgaat
     4321 ggttgggata tccgcgcaga gggctattta cctgcctggc cgcagcttgg cgcaagcctg
     4381 atgtatgaac agtattatgg cgatgaagtc gggctgtttg gtaaagataa gcgccagaaa
     4441 gacccgcatg ctatttctgc cgaggtgacc tatacgccag tgcctcttct gacactgagc
     4501 gccgggcata agcagggcaa gagcggtgag aatgacactc gctttggcct ggaagttaat
     4561 tatcggattg gcgaacctct ggagaaacaa ctcgatacag acagcattcg cgagcgtcga
     4621 atgctggcag gcagccgcta tgacctggtt gagcgtaata acaacatcgt tcttgagtac
     4681 cgcaaatctg aagtgatccg tattgctctg cctgagcgta ttgaaggtaa gggcggtcag
     4741 acactttccc tggggcttgt ggtcagcaaa gcaactcacg gactgaaaaa tgtgcagtgg
     4801 gaagcgccgt cattactggc tgaaggtggc aaaattaccg gtcagggtag tcagtggcaa
     4861 gtaacgctcc cggcttatcg tccaggcaaa gacaattatt atgcgatttc tgcggttgcc
     4921 tacgataaca aaggcaatgc ctcaaaacgc gtgcagacag aggtggtcat taccggagca
     4981 ggtatgagtg ccgatcgcac ggcgttaacg cttgacggtc agagccgtat tcaaatgctt
     5041 gctaacggta atgagcaaag accgctggtg ctgtctctgc gcgacgccga gggccagcca
     5101 gtcacgggca tgaaagatca gatcaagact gaactaactt tcaaaccggc tggaaatatt
     5161 gtgactcgta ccctgaaggc cactaaatca caggcaaagc caacactggc tgagttcacc
     5221 gaaactgaag caggggtgta tcagtctgtc tttactaccg gaacgcagtc aggtgaggca
     5281 acgattactg ttagtgttga tggcatgagc aaaaccgtca ctgcagaact gcgggccacg
     5341 atgatggatg tggcaaactc caccctgagc gctaacgagc cgtcaggtga cgtggttgct
     5401 gatggtcagc aagcctacac gctgacgctg actgcggtgg atactgatgg taacccggtg
     5461 acgggagagg ccagccgctt gcgatttgtt ccgcaagaca ctaatggtgt caccattggt
     5521 acaatttcgg agataaaacc aggcgtttac agcgctacgg tttcttcgac ccgtgccgga
     5581 aacgttgttg tgcgtgcttt cagcgagcag tatcagctgg gcacattaca acaaacgctg
     5641 aagtttgttg ccgggccgct tgatgcagca cattcgtcca tcaccctgaa tcctgataaa
     5701 ccggtggttg ggggtacagt tacggcaatc tggacggtaa aagatgccta tgacaaccct
     5761 gtgaccagcc tcacgccgga agcgccgtca ttagcgggtg ccgctgctgt aggttctacg
     5821 gcatcgggct ggacaaataa tggtgatggg acgtggactg cgcagattac tctcggctct
     5881 acggcgggtg aattagaagt tatgccgaag ctaaatggac aggatgcggc agcaaatgcg
     5941 gcaaaagtaa ccgtggtggc tgatgcgtta tcttcaaacc agtcgaaagt ctctgtcgca
     6001 gaagatcacg taaaagccgg cgaaagtaca accgtgacgc tggtggcgaa agatgcgcat
     6061 ggcaacgcta tcagtggtct ttcgttgtcg gcaagtttga cggggaccgc ctctgaaggg
     6121 gcgaccgttt ccagttggac cgaaaaaggt gacggttcct atgttgctac gttgactaca
     6181 ggcggaaaga cgggcgagct tcgtgtcatg ccgctcttca acggccagcc agcagccacc
     6241 gaagccgcgc agttgacggt catcgccgga gagatgtcat cagcgaactc tacgcttgtt
     6301 gcggacaata aggctccgac cgtcaaaacg acgacggaac tcaccttcac cgtgaaggat
     6361 gcgtacggga acccggtcac cgggctgaag ccagatgcac cagtgtttag cggtgccgcc
     6421 agcacgggga gtgagcgtcc ttcagcagga agctggacag agaaaggtaa tggggtctac
     6481 gtgtcgacct taacgctggg atctgccgcg ggtcagttgt ctgtgatgcc gcgagtgaac
     6541 ggccaaaatg ccgttgctca gccactggtg ctgaacgttg caggtgacgc atctaaggct
     6601 gagattggtg atatgacagt gaaggttaat aaccaactgg ctaatggaca gtctgctaac
     6661 cagataaccc tgaccgtcgt ggacagctat ggtaacccgt tgcaggggca agaagttacg
     6721 ctgactttac cgcagggtgt gaccagcaag acggggaata cagtaacaac caatgcggca
     6781 gggaaagtgg acattgagct tatgtcaacg gttgcgggag aacacaatat ttccgcttcg
     6841 gtgaatggtg ctcagaagac ggtcacggtg aaatttaacg cggatgccag caccggtcag
     6901 gcaaacctgc aggtagacac cgccgttcaa aaagtggcaa acggcaaaga cgcctttacg
     6961 ctgacggcga ccgttgagga taaaaatggt aaccctgttc cagggagcct ggtgaccttt
     7021 aatctgcccc ggggtgtcaa gccgcttaca ggcgataatg tctgggtgaa agccaacgat
     7081 gaggggaaag cagagttgca ggtggtttca gtgactgccg gaacctatga gatcacggcg
     7141 tcggcaggaa atgaccagcc ttcgaatgcg cagtctgtaa cgtttgtggc ggataagact
     7201 acggcaacta tctccaatat tgaggtgatt ggcaactatg cgctggcgga cggcaaagcc
     7261 aaacaaacgt ataaagttac ggtgactgat gccaataaca atttggtgaa agatagcgaa
     7321 gtgacgctga ctgccagccc ggcttcgtta aacctggaac cgaatggcac tgcgacaacg
     7381 aatgagcaag ggcaggctat tttcaccgct accactattg ttgcggcgac atacacactc
     7441 aaggcgcaag tgagtcagac caacggtcag gtatcaacga aaactgccga atctaaattc
     7501 gttgcggatg ataaaaacgc ggtactcacc gcatcatctg atatgcaatc tctggtggcg
     7561 gatgggaaat cgactgcgaa gctggcggtg accctgatgt cggcgaataa ccccgttggc
     7621 gggaatatgt gggtcgacat tcagacgccg gaaggggtga cggagaagga ttatcagttc
     7681 ctgccgtcga aaaatgacca tttcgtgagc gggaaaatca tacgtacatt tagtaccagc
     7741 aagccaggcg tctatacatt cacatttaac gccctgacct atggcgggta cgaaatgaag
     7801 ccagtgacgg tgaccattac cgcggtggat gccgatacgg caaagggcga ggaggcgatg
     7861 aaataatttt taagaatatg aaaatagcat tctaataaac aataatggcg ttgacatatt
     7921 caacgccatt atttattgaa ttgcctgaca gtagtctcgt tataagttca tttagatata
     7981 gcgacagcta aaccggacga acggggataa aaatttcgca aacaacatca ttgctatcct
     8041 tttctacgaa atcttcattg taataaaatc gctcaatatc actgccgttc actctggcta
     8101 accccctggc aggtaattcc atcagataga ggtttcgcga ccattcagga tagcgctccc
     8161 actcaccttc atagcggaaa gatgcataca gtcctcggtt tattgtccac tctttcgcca
     8221 tctcagaatc ggtttgttta cataccgtac cggcaaaagt ttcaactgtg agcaaatcga
     8281 ccgattttgt tgacggttca aacgtggaga cgaaatagat atccgtctta aaattattca
     8341 tacatctggc taaatgatga cgtcgcagtt tgacttcttc atccggtgat ttattcgaca
     8401 cttcaatgtc atatttaaat gagttgccaa aaataggtgt ctcaggaaaa ttgactaatc
     8461 tacactccgt cttttgctgt tgacgtatta aaaaagaagg gaagatattt gcgagatccc
     8521 aataatcacg gtggcggtat tcacggggag agcagccgaa taacttttta aattcgcggc
     8581 tgaatgactg ctgtgaatca aaatgcaaag agagtgcaat atcaagcata gatttcgcgg
     8641 tgagccggac aagaatagcg gcacggcaaa gccttcgttt gcgaatgtat tctcccaaag
     8701 gcacatgcat gaaatttcgg aacagaagct ggatgttgcg tctgctgtag ccagatttct
     8761 gtgcgatatc ttcgattgaa atagggtgct caagattgca ctcaatccac tccaggagct
     8821 gctgtagaat cttctgcctg atcatcacat tctccattga ttcttcgtca tatcgcacat
     8881 tttcgttatg ccctgggccc taagtgtatc aaaaacagca agtccgaagg caaaagcact
     8941 ggtagcgtct gcttcgttgt ccccgaaaat agagaggttt cagtcctaca ttattaatga
     9001 attttttgca taagtgatat caaaatccac atactaattt gaggttacgt tttaacgtag
     9061 actcattgtt catgcctaat ggagggactg acagtggaat tttccgtatt aagtaacaat
     9121 ctgaaaatgc cgatgatggg atttggtgtt tttcaggtta ccgataaaaa cgtgtgcaaa
     9181 cagtcagtgt tgaacgctat ccgcacgggt tatcgactca tcgacactgc agcggtatac
     9241 ggtaatgaag atgccgtcgg tgaagccgtt cgtgaagcta tatctgaagg tttatgtacc
     9301 cgagatgagt tatttataac gtctaaattg tgggggcagg atatgttgaa tcaggatacg
     9361 gcagcagcag gtattgaagc atcattaaaa aaatcgggac tagaatactt cgacctctat
     9421 ttattgcacc aggctatgcg cgattatttc agtgcgtggc gtgcactcga agatgcctat
     9481 gaagagggca aattaaaagc aattggggtt tccaatttct atcctcatgt tctggcgaac
     9541 ttttgtgaaa cggtaagagt taaaccgatg gtcaaccagg tcgagttgca tccctatttt
     9601 gcccaaccag aggcgctggc aaccatgaag tattataacg tgcagcctga agcatgggct
     9661 ccgttaggtg gtggacgaca taaacccttt gaaaataatc tgcttcagag tattgcagat
     9721 gcccatcaaa aatcgatttc tcaagtcatt ctgcgttgga atattcaacg gggagtggtc
     9781 gttattccga aatcgacaca tcaacagcgt atcgaagaaa attttgctat ctgggatttc
     9841 tcactgacag agaaagaaat ggcacaaatt agttcgcttg atttgggtta tgttggggag
     9901 tcggtaaaac attttaatcc tgaatttgtt cgtggttgtc ttgctgtaaa aaattcatga
     9961 ttgatattaa tcatatattt tacctgagac gacaagaatc ttttaacagg ggagtgatat
    10021 tgatcttcac tctgtcatat ctccggtaat atggcgtcag gcttcatcat cagaaagggg
    10081 gcctgacgcc tgaaaaagtg aacaacagac agtgttcgga ttatcaatat tcagttttta
    10141 acgttgaact ggattcatta ctgcgttgtt taagaatttc ccgcgccgaa tctgccatta
    10201 tcatgacagc acctgccagc atcagagtat ctttcaatac caggcgacca gcaccggata
    10261 aataagggaa accatgatga gcgtcaccca atgcgggtac ccatgcctcc ggggtggtga
    10321 ttaaaaatga gagtgttacc aacggtgtgg taaatgccat cagccctccc aataaaccta
    10381 accagcgatt gacaggatta gccaaaacca gcagagcaat aatcacctcc acgacgccaa
    10441 gaccgttgga aaaaccatag gtattattgg ccgattgcca tgcccttgct tctggtttgt
    10501 attcgccttc gtgagtcaga tactgtttat agtcttccgg gtgttcataa aagaacgaca
    10561 ttagtggact gtttgcgacg aatggtgtaa tgctgtctgc ctcgtaaggg acaaacttta
    10621 ataacccaat ccacataaaa acaatcgcaa tactcagacg aatcaatgtc aggccaattt
    10681 tatctccccg acttaacatg tgcaggtatt tctccattgt aaacatcctt atttatacaa
    10741 tactgctgtt gcggttgaat tattatcaat tcgcatcgat gtgactttcc aggatgaagc
    10801 attatatttt ctggctatat cagcaatggc atcctccaga actgtcggtg atgctgatac
    10861 tgacgagacg ctgatatgtt tcagaattat tttatcatct gcattggtgg aaaatgccat
    10921 aacgccagat aatagtgttg caaataaaac agattttttg aacatataaa ataccatcaa
    10981 tgaagtgatt aagaagttat agagtaacag ggaattaata agattctttt ctctgagacg
    11041 ccagaatatt tgttctggcg tctgattttg agtttatttg actaatgaaa atagatcatt
    11101 gagtgattcg ctcatcgacg gatgagtaaa tatctgatcg cgtaatatgc tataaggcag
    11161 cccggcatcc atcaccattt tcactatatt gatcatctcg tgggagtcaa cacacagcag
    11221 tgatgcccct aatatacgtt gggttttatt atcaacaatc gcttttaata ccccacgagt
    11281 atcattcatc actctggcac gcggaattgc agctacaggc aatgtcacca cctgaatatc
    11341 agcaccactc tctctggcct gttcttctgt cataccaacc ctggacagtg gcggtgtcat
    11401 gaatacggaa taaggcacat ttttccgatc atcagtacta cgtctgcctt ctcccagcaa
    11461 ctcatcacgc acaatgcggt aatcatccag tgaaatataa gtaaattgca gcccgccggt
    11521 aacatctccc atcgcccaaa tattgtctgc ggtggtatgt aattgcttgt caacgacaat
    11581 tgccccgcgc tcgtttactg cgataccagc attttctgga tgtaacgaag cggtagccgg
    11641 ttgacgaccg gaagctatta acagtgcatc caccgccagt tgggcgtgct cgctatgcac
    11701 ttgcacttga ttttcatggt gactgattcg ctccacatgg gcattgagga taatatcgac
    11761 gccctgatcg cgtaaaatcg tcgcgatatt atcagcaata tcccgatctt cccgaggcaa
    11821 aaacagcgaa gctgcttcta aaatggttac cttgctgcca aaattagcga acatagaggc
    11881 gaactcaacg ccaatatatc cgccgcccaa aatacctaaa tgcccaggca attcttttag
    11941 attaagtaat ccggtgctgt catatacacc aggcgtggtg gtaattccag gaattggcgg
    12001 aaccacggtt tgtgcaccgg tattaataaa aattttctcg ccatgaattt ccagatttcc
    12061 ctcaggccga tgaacacgca ggctatgatt attgataaac tccgcctggc cgtcgatcac
    12121 gtcgatattg ggcatatccg caagattatg aaaattctta ttacgtaaaa aattaaccac
    12181 ttcattttta cgctgtatgg cacggacaaa atctgtgtgc tgctgtgcgt catgaaccaa
    12241 tgttttggtt gggatgcagc cgatattaat acaggtcccg ccatacattg catttgattg
    12301 ttcaatgaga gccactcgcc aacctgcttt tgccagcgtg acggctaatg tttttccagc
    12361 cttgccaaaa ccaataataa ctgcctgata tttattcatt atgatttcct gtcgaaacgc
    12421 gttcatttga catcatgact atagacgcaa aagagcaaat accatttctt cacaacgctt
    12481 gatattttgt cttatcgtct ggtattttct ttttcttaat
//
LOCUS       DAZFVO020000142        12508 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFVO020000142 DAZFVO020000000
VERSION     DAZFVO020000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.143"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2442
                     /locus_tag="ACHKNB_RS19285"
                     /old_locus_tag="pgaptmp_003857"
     CDS             <1..2442
                     /locus_tag="ACHKNB_RS19285"
                     /old_locus_tag="pgaptmp_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4197282.1"
                     /translation="YRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISLLKEKGV
                     IAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQSLPLIN
                     QCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVLMDYNLF
                     ASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEISRTYLFR
                     PLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGIAQTNAT
                     VTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAASTPFLT
                     RQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDDYHSAAI
                     GIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLAAYRFSD
                     RHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWNADASTT
                     ANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVGYDMQNS
                     SHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDISGTYAA
                     NDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDYTNHFGI
                     AVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVII
                     RNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLP
                     EHMEDTANRLILPCH"
     gene            2457..3188
                     /locus_tag="ACHKNB_RS19290"
                     /old_locus_tag="pgaptmp_003858"
     CDS             2457..3188
                     /locus_tag="ACHKNB_RS19290"
                     /old_locus_tag="pgaptmp_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HGU4197283.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3185..4246
                     /locus_tag="ACHKNB_RS19295"
                     /old_locus_tag="pgaptmp_003859"
     CDS             3185..4246
                     /locus_tag="ACHKNB_RS19295"
                     /old_locus_tag="pgaptmp_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4197284.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKILVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            4398..5444
                     /locus_tag="ACHKNB_RS19300"
                     /old_locus_tag="pgaptmp_003860"
     CDS             4398..5444
                     /locus_tag="ACHKNB_RS19300"
                     /old_locus_tag="pgaptmp_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGU4197285.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(5441..6232)
                     /gene="nei"
                     /locus_tag="ACHKNB_RS19305"
                     /old_locus_tag="pgaptmp_003861"
     CDS             complement(5441..6232)
                     /gene="nei"
                     /locus_tag="ACHKNB_RS19305"
                     /old_locus_tag="pgaptmp_003861"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HGU4197286.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6268..7002)
                     /gene="pxpA"
                     /locus_tag="ACHKNB_RS19310"
                     /old_locus_tag="pgaptmp_003862"
     CDS             complement(6268..7002)
                     /gene="pxpA"
                     /locus_tag="ACHKNB_RS19310"
                     /old_locus_tag="pgaptmp_003862"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HGU4197287.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(6992..7924)
                     /gene="pxpC"
                     /locus_tag="ACHKNB_RS19315"
                     /old_locus_tag="pgaptmp_003863"
     CDS             complement(6992..7924)
                     /gene="pxpC"
                     /locus_tag="ACHKNB_RS19315"
                     /old_locus_tag="pgaptmp_003863"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HGU4197288.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(7918..8574)
                     /gene="pxpB"
                     /locus_tag="ACHKNB_RS19320"
                     /old_locus_tag="pgaptmp_003864"
     CDS             complement(7918..8574)
                     /gene="pxpB"
                     /locus_tag="ACHKNB_RS19320"
                     /old_locus_tag="pgaptmp_003864"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HGU4197289.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(8597..9340)
                     /gene="ybgI"
                     /locus_tag="ACHKNB_RS19325"
                     /old_locus_tag="pgaptmp_003865"
     CDS             complement(8597..9340)
                     /gene="ybgI"
                     /locus_tag="ACHKNB_RS19325"
                     /old_locus_tag="pgaptmp_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HGU4197290.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9611..11092
                     /gene="dtpD"
                     /locus_tag="ACHKNB_RS19330"
                     /old_locus_tag="pgaptmp_003866"
     CDS             9611..11092
                     /gene="dtpD"
                     /locus_tag="ACHKNB_RS19330"
                     /old_locus_tag="pgaptmp_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HGU4197291.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11134..>12508)
                     /gene="phrB"
                     /locus_tag="ACHKNB_RS19335"
                     /old_locus_tag="pgaptmp_003867"
     CDS             complement(11134..>12508)
                     /gene="phrB"
                     /locus_tag="ACHKNB_RS19335"
                     /old_locus_tag="pgaptmp_003867"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HGU4197292.1"
                     /translation="DNLALAAACRNSSARVLALYIATPRQWATHNMSPRQAELINAQL
                     NGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVEV
                     ERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKV
                     RSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQNGAGEYEQQRDFPAVE
                     GTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPS
                     LCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQLNSTGWMHNRLRMITA
                     SFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGE
                     KFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQPIVDHKEARLRTLAAY
                     EEARKGA"
BASE COUNT         2956 a         3414 c         3055 g         3083 t
ORIGIN      
        1 tatcgactct cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa
       61 ttcaacctta atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag
      121 gaaaaaggag tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa
      181 atcagtaacg ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc
      241 aatgatttac tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg
      301 ataaatcagt gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc
      361 aatcagcaac taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg
      421 accccaccct ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt
      481 gccagcagct accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca
      541 gccggaatca acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat
      601 agcgatgata accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta
      661 ccgcaattag gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac
      721 ggtttttctt ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta
      781 cgcggctacg ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt
      841 caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac
      901 cttaatcagt ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg
      961 aacaatttcc aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc
     1021 tataaactgg ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc
     1081 ttttttagca atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg
     1141 ctgctttctg gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg
     1201 cttggcgcgc tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac
     1261 gagcggggct taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg
     1321 atttccctcg ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg
     1381 gatcacaaat acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg
     1441 ggccaaccga ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg
     1501 tggaatgcag acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt
     1561 gactggagag atatctctat ttctacgtca ttcaatacaa cccattacga agataaagat
     1621 cgcgacaacc agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc
     1681 tatgacatgc aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg
     1741 gatgaacgta atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga
     1801 gcccaggtga gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt
     1861 acctatgccg ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca
     1921 acccaatatg gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc
     1981 agcaccgatg gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt
     2041 ggcattgcgg tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg
     2101 aatgacttac ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa
     2161 ggcgcgatag gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt
     2221 cgcaacgcca gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc
     2281 attagcgtgg ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat
     2341 caaaagttta ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat
     2401 atggaagaca ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga
     2461 catttatgaa aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac
     2521 tacaacccga tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg
     2581 tggataatcg tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag
     2641 gggagaaaag cgatgatctt ctggtcgccc taccgccgat acaacgtctg gaaccgaaag
     2701 caacaacgca ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg
     2761 aaacgctatt tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc
     2821 atgccgttct gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat
     2881 tacgcaagaa agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc
     2941 aactgacact gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg
     3001 aaaaaggcgt tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca
     3061 caaacaccgg aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg
     3121 cgttacgcat gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga
     3181 agaaatgagt gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa
     3241 gtctgcgctt gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga
     3301 agcgattcaa ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc
     3361 tgacgatatt aaaattctcg tctattgtga caacaatacc aacggcaatt ttgaaagtga
     3421 gcatgtttat gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg
     3481 cgtaacttac aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa
     3541 ccagtgtatt gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg
     3601 atggcagaac aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct
     3661 cgctcgcatg cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac
     3721 gcttagcgac tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagaccctac
     3781 tgcccaaaac ctgaaatatc atattactgg tctggaaaac attcgcgtgc tggattgcag
     3841 cgtcaatttt tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct
     3901 ggatattcgt cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa
     3961 tgaccaatgc accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt
     4021 tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa
     4081 cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt
     4141 ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc
     4201 tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc
     4261 ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat
     4321 tgctgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa
     4381 taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc
     4441 tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga
     4501 tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc
     4561 tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca
     4621 ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca
     4681 gcgccatcgt gggttggtta ttggtgcgct atagctcatt gcctggaaat accggtgcct
     4741 ggggttcttc tcctggcggc gcggcggcaa tggtcgctat ggcgcaagat tacggcgcag
     4801 atattcgcct ggttgcattt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg
     4861 ttttggtcac ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct
     4921 ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcggtc gttgccggta
     4981 cggcgggatg cttgctacgt tttccttccg gcacgatgct catcccgatg ctggcgggcg
     5041 cagtgctcca gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg
     5101 cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg
     5161 cactacgccc gctaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcgg
     5221 gtatggcgtg ggggctgacc cggtttatgc acattgattt tatgactgcc tacctcgcca
     5281 ccagtcccgg cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg
     5341 cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca
     5401 ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac
     5461 accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc
     5521 acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct
     5581 catccacctg cccccgcgta gcgtaagaaa accgaggaat atccagtaac gcgtgtgcga
     5641 gtgcatccag ttgcgccgca ttgaggtctt tcgctttatg atttccagtc aacccaacct
     5701 gccagaggat ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgatcgagca
     5761 gtaatccagc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct
     5821 ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg
     5881 tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt
     5941 cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa
     6001 ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat
     6061 gagttaacaa cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg
     6121 attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga
     6181 tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat
     6241 ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct tttcggcaaa
     6301 tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat gcaggcagac
     6361 tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac
     6421 catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc ctggctgact
     6481 tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt
     6541 tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaac
     6601 caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt
     6661 cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc
     6721 acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg
     6781 cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc
     6841 aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gggcatcgcc
     6901 tgcatgaaat ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct
     6961 ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc cgccacgcta
     7021 attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc agtgaacact
     7081 ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa
     7141 tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatag
     7201 gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat aacagttcgc
     7261 gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg
     7321 ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat
     7381 attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg
     7441 cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac
     7501 ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg
     7561 gaacatcaat accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa
     7621 gcgttaaacg ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat
     7681 ccagccgcgc ttcgcaaccg gcccctgtca gagcaaacca cccatcagtt tcgaactcaa
     7741 ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagca
     7801 ggttagcaat gcgcagcgca ggcatatcca gtgcgccgca gtggctgata cccgactggc
     7861 gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca
     7921 gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg
     7981 ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc
     8041 cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg
     8101 aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag
     8161 atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa
     8221 ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc
     8281 gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc
     8341 gctctcctcc caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt
     8401 acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc
     8461 caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc
     8521 cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga
     8581 tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg
     8641 tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac
     8701 ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt
     8761 cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc
     8821 cgcccgtgca ccaggcgacg cgcaggacca cctcaggtcc ggtatcgcca caccataacg
     8881 gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg
     8941 tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg
     9001 ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat
     9061 acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga
     9121 ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac
     9181 gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg
     9241 tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca
     9301 gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct
     9361 gatagctaca ccgttaacat agcgttacct cgccagttcg tcgatagcca atcttttgac
     9421 atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc
     9481 taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt
     9541 tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg
     9601 tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg tcgttgcgct
     9661 gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac
     9721 caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct
     9781 ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat
     9841 ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg gtgccagtga
     9901 gatccatccg tcattcctct atctgtccct ggcgataatc gtttgtggct atggtctgtt
     9961 taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga
    10021 tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctattgc
    10081 ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg
    10141 tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg
    10201 cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg gatggctgct
    10261 ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag agtggtcggt
    10321 atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa
    10381 agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag
    10441 tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg
    10501 cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa
    10561 tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc
    10621 aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc tgatgagcgc
    10681 cgggttctgc attctgacct taagcgcccg ctggtcctca atgtatggtc actcttctct
    10741 gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt
    10801 tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat taaccggcat
    10861 ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg ccgatcagac
    10921 atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga
    10981 agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg
    11041 gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggaatctt aataggccag
    11101 caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta
    11161 cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag
    11221 catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc
    11281 gcgcagctcc ggtaaccatc ggcggataaa ctcgccctca cggtcaaatt tctcgccctg
    11341 agttatcgga ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca
    11401 ctgccagcca ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg
    11461 ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg
    11521 tagccggtta tgcatccagc cagtactgtt aagctgacgc atagcggcat caacaatcgg
    11581 gtatcccgtt ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac
    11641 acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag
    11701 atgacggtag aactcacgcc agatcagctc actaagccag acactaccgg ccccaccgtc
    11761 cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc
    11821 cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg
    11881 ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc
    11941 ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg
    12001 cgcgggctct atcgatccgc tactacgaac ttttggcgca gcgacacact ccggcatccc
    12061 ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg
    12121 attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac
    12181 cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg
    12241 atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac
    12301 actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc
    12361 tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg
    12421 cgtcgcccac tggcgtggtg tagcgatata taacgccagc acgcgtgcag acgaattgcg
    12481 gcaggcggca gccagtgcga gattatcg
//
LOCUS       DAZFVO020000143        12407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFVO020000143 DAZFVO020000000
VERSION     DAZFVO020000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.144"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..607
                     /locus_tag="ACHKNB_RS19340"
                     /old_locus_tag="pgaptmp_003868"
     CDS             <1..607
                     /locus_tag="ACHKNB_RS19340"
                     /old_locus_tag="pgaptmp_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4197293.1"
                     /translation="INNNLLSEIKIFYSNFCNVQTLNAANNQLKYINLDFLTYLPSIK
                     SLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPA
                     LKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKV
                     KNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            931..2319
                     /gene="narU"
                     /locus_tag="ACHKNB_RS19345"
                     /old_locus_tag="pgaptmp_003869"
     CDS             931..2319
                     /gene="narU"
                     /locus_tag="ACHKNB_RS19345"
                     /old_locus_tag="pgaptmp_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HGU4197294.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALCFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2401..6141
                     /gene="narZ"
                     /locus_tag="ACHKNB_RS19350"
                     /old_locus_tag="pgaptmp_003870"
     CDS             2401..6141
                     /gene="narZ"
                     /locus_tag="ACHKNB_RS19350"
                     /old_locus_tag="pgaptmp_003870"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HGU4197295.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYTAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6138..7682
                     /gene="narH"
                     /locus_tag="ACHKNB_RS19355"
                     /old_locus_tag="pgaptmp_003871"
     CDS             6138..7682
                     /gene="narH"
                     /locus_tag="ACHKNB_RS19355"
                     /old_locus_tag="pgaptmp_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4197296.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7682..8377
                     /gene="narW"
                     /locus_tag="ACHKNB_RS19360"
                     /old_locus_tag="pgaptmp_003872"
     CDS             7682..8377
                     /gene="narW"
                     /locus_tag="ACHKNB_RS19360"
                     /old_locus_tag="pgaptmp_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4197297.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQRKAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8374..9054
                     /gene="narI"
                     /locus_tag="ACHKNB_RS19365"
                     /old_locus_tag="pgaptmp_003873"
     CDS             8374..9054
                     /gene="narI"
                     /locus_tag="ACHKNB_RS19365"
                     /old_locus_tag="pgaptmp_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4197298.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9133..10026
                     /locus_tag="ACHKNB_RS19370"
                     /old_locus_tag="pgaptmp_003874"
     CDS             9133..10026
                     /locus_tag="ACHKNB_RS19370"
                     /old_locus_tag="pgaptmp_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HGU4197299.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(10122..10967)
                     /gene="nhoA"
                     /locus_tag="ACHKNB_RS19375"
                     /old_locus_tag="pgaptmp_003875"
     CDS             complement(10122..10967)
                     /gene="nhoA"
                     /locus_tag="ACHKNB_RS19375"
                     /old_locus_tag="pgaptmp_003875"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HGU4197300.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11140..11709
                     /locus_tag="ACHKNB_RS19380"
                     /old_locus_tag="pgaptmp_003876"
     CDS             11140..11709
                     /locus_tag="ACHKNB_RS19380"
                     /old_locus_tag="pgaptmp_003876"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HGU4197301.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11713..11940)
                     /gene="pptA"
                     /locus_tag="ACHKNB_RS19385"
                     /old_locus_tag="pgaptmp_003877"
     CDS             complement(11713..11940)
                     /gene="pptA"
                     /locus_tag="ACHKNB_RS19385"
                     /old_locus_tag="pgaptmp_003877"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HGU4197302.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(12039..>12407)
                     /locus_tag="ACHKNB_RS19390"
                     /old_locus_tag="pgaptmp_003878"
     CDS             complement(12039..>12407)
                     /locus_tag="ACHKNB_RS19390"
                     /old_locus_tag="pgaptmp_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HGU4197303.1"
                     /translation="LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAGRRLS"
BASE COUNT         2998 a         3171 c         3165 g         3073 t
ORIGIN      
        1 tattaacaac aatttgcttt ctgaaattaa aatattctat agcaacttct gcaatgttca
       61 gacattaaac gctgctaaca accagttgaa atatataaat cttgatttcc tgacttatct
      121 tccatctatc aaaagtttaa gactggacaa taataaaata acccacattg atactaataa
      181 tacatccgat attggaactt tattccccat aataaaacag agcaaaaact taaatttttt
      241 aaatgtttct gggaagaaca attgccctac tatgcagctc atgttattta atttgttttc
      301 cccagcactt aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca
      361 ctctgacgga atagatgtgg ataacgaatt gtttcactat cctattaaaa aagcatatac
      421 cccatataat atacacactt acaagacaga ggaagttgta aaccagagga atataaaagt
      481 taaaaacatg accttagatg aaataaacaa tacttactgt aataacgatt attacaatca
      541 ggcaataaga gaggaaccga tagaccttct ggacagatcg ttttcctcca gttcatggcc
      601 tttttagtga ataaatcatc actaattcta taagtgtctg gtcttgttga taatatagtt
      661 aatatgtaac cctttagatt cacgatctga agggttgaaa catgacatac gttcaaagat
      721 taacccccgt ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta
      781 atccccattc tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc
      841 caattcatcc ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt
      901 cattaccaat gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt
      961 tatcttttac gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat
     1021 attgctcgaa gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg
     1081 atgctattta gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat
     1141 caactctttt tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac
     1201 tcctttatgg tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg
     1261 attattcctt gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata
     1321 tttatcgtta tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc
     1381 aatatcagtt tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga
     1441 ttaggaaact taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct
     1501 gtatttgcct ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg
     1561 gcgaatgccg catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcatggtca
     1621 gggatgaatg atatcgccag ttcacgcgcc tcaattgccg accagctccc tgtcttacaa
     1681 cgcctgcatc tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt
     1741 ttttctgcgg gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc
     1801 ctggcgttct ttggcccatt tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc
     1861 gataagttcg gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt
     1921 gccctgctgt tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc
     1981 gtatttatgg ggctgtttct gactgcgggt ctgggaagtg gttctacttt ccagatgatc
     2041 gccgtcatct ttcgccagat aaccatttat cgggtaaaga tgaaaggcgg tagtgatgag
     2101 caagctcaga aagaagccgt caccgaaacg gcggcggctc tgtgctttat ctcagccatt
     2161 ggcgcagtgg gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc
     2221 tctccggtcg gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc
     2281 tggctggttt atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca
     2341 accgtgtcgc atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc
     2401 atgagtaaac ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat
     2461 ggtcacggac aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg
     2521 cagttcgaca aaatcgtgcg ttccacccac ggtgttaact gtacaggctc ctgtagctgg
     2581 aaaatctacg ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc
     2641 actcgccctg acctgcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc
     2701 tggtatcttt acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa
     2761 ctgtggcgcg aagccctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg
     2821 aacgatccgc aaaagtgcct gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc
     2881 tccaactggc aggaactaaa ccagctgatt gccgccgcta acgtctggac catcaaaacc
     2941 tacggcccgg atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac
     3001 accgccggaa cgcgttatct gtcgctgctt ggcggcacct gtttaagttt ctacgactgg
     3061 tattgcgacc tgccgcccgc ctcgccgatg acctggggcg agcaaaccga cgtaccggaa
     3121 tctgccgact ggtataactc cagctacatc atcgcctggg ggtctaacgt accgcagaca
     3181 cgtacgccgg acgcccactt ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc
     3241 attacccctg actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa
     3301 ggcactgata gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc
     3361 gataatccca gcgactactt tatcaactac tgccgccgct acagcgacat gccgatgctg
     3421 gtaatgctgg agcctcgcga cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct
     3481 gacctggtgg atggactggg cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat
     3541 accgcaggtg aattggtagt gccgaacggt tcgattggtt tccgctgggg agaaaaaggc
     3601 aaatggaatc tggaatccat tgccgccggt acggaaaccg aattgtcgtt aaccctgctc
     3661 ggtcaacatg acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg
     3721 cattttcgca gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg
     3781 acgttagtcg atggcaacac ctgtccggtg gtcagcgttt atgatttggt actggcgaat
     3841 tacggcctcg atcgcgggct ggaagatgaa aacagtgcga aagattacgc tgaaatcaaa
     3901 ccgtacaccc cagcctgggg tgagcaaatt accggcgtgc cgcgccagta tattgaaacc
     3961 atcgcccgtg aatttgccga taccgcccat aaaacgcatg ggcgctcgat gattatcctc
     4021 ggcgcaggtg ttaaccactg gtatcacatg gacatgaact accgcgggat gatcaatatg
     4081 ctgatcttct gcggttgtgt cgggcaaagc ggtggcggct gggcacacta tgtcggccag
     4141 gaaaaactgc gcccacaaac cggctggttg ccgctggcct ttgcgctcga ctggaaccga
     4201 ccaccacgcc agatgaacag tacctcgttt ttctacaatc attccagcca atggcgctat
     4261 gaaaaagtct ctgctcagga gttactttca ccgctcgccg atgccagtaa gtacagcggt
     4321 catctgattg atttcaacgt tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag
     4381 ctggggcgta acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca
     4441 gaatttaccg cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat
     4501 agcggcagca atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc
     4561 ggcaaaggcc acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc
     4621 gaggaactcg gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg
     4681 attgaaggca agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg
     4741 ttctccgata tcgttctgcc caccgccacc tggtacgaaa aagacgatat gaacacctcg
     4801 gatatgcatc cgtttattca tccgctttct gcggcggtcg atccggcgtg ggaatcacgc
     4861 agcgactggg aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat
     4921 cttggcaaag aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc
     4981 tcacagccgt gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa
     5041 accgcgccga atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc
     5101 tcgctcgggc cattgatgga caaacttggc aatggcggta aagggatttc gtggaatacc
     5161 caggatgaaa tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag
     5221 gggcgtccac tgattgacac cgctattgat gcatcagagg tcattctggc actggctcca
     5281 gaaaccaacg gtcatgttgc agtcaaagcg tggcaggcgc tgggcgaaat caccggacgc
     5341 gaacataccc atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag
     5401 gcgcagccac gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcatgtt
     5461 tcctataacg cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga
     5521 cgccagcaac tctatcagga tcatccgtgg atgcgagctt ttggtgaaag cctggtggct
     5581 tatcgcccgc ctattgacac ccgtagcgtc agtgaaatgc gccaaatccc gccaaacggc
     5641 ttcccggaaa aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc
     5701 tacagtgaaa acctgctaat gctgacgctt tctcgcggtg gaccgattgt ctggatcagc
     5761 gaaaccgatg cccgagaatt gaccattgtc gataacgact gggtggaagt gtttaacgcc
     5821 aatggcgcgc tgacggcccg cgcggtggtc agccaacgtg taccgccggg tatgaccatg
     5881 atgtatcacg cccaggaacg cattatgaat atccctggtt cggaagtaac tggcatgcgc
     5941 ggcggcattc ataactcggt tacccgcgtt tgcccgaaac caacgcatat gattggcggt
     6001 tacgcgcagc tggcctgggg ctttaactac tacggcaccg tcggctcaaa ccgtgatgaa
     6061 ttcatcatga tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag
     6121 gtacaggagg cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgata
     6181 aatgtatcgg ctgccatacc tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag
     6241 gcatggagta cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa
     6301 actgggaaga tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatac
     6361 gcccgcgtct gggcagtaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc
     6421 cgcagattga tgattactac gaacctttca ccttcgacta cgaacatttg catagcgcac
     6481 cggaaggcaa acatatccct actgctcgcc cgcgttcgct gattgacggt aagcggatgg
     6541 acaaagtgat ctgggggcca aactgggaag aactgctggg cggcgagttt gaaaaacgtg
     6601 cccgcgaccg caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct
     6661 tcatgatgta cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct
     6721 gcccaagcgg cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata
     6781 aatgccgtgg ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact
     6841 ggaaaagcgg caagtcagaa aaatgtatct tctgttaccc acgaattgag tccggtcaac
     6901 cgaccgtttg ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg
     6961 acgccgaccg cattgaggaa gcggcaagca ccgagcgcga ggttgacctc tatgaacgcc
     7021 agtgcgaagt gttcctcgat ccgcacgatc cctcagtgat cgaggaagcc ctgaaacaag
     7081 gtattccaca aaatgtgatt gacgctgccc agcgttcgcc tgtctacaaa atggcgatgg
     7141 actggaaact ggcgctaccg ttgcaccctg aataccgcac cctgccgatg gtctggtacg
     7201 ttcctccgct gtcaccgatt cagtcttacg ctgatgcggg cggtttgccg aaaagcgaag
     7261 gcgtgctccc cgccatcgaa agcctgcgta ttccggtgca atatctcgcc aatatgttga
     7321 gtgccggtga tactggtccg gtactgcggg cgctgaaacg gatgatggcg atgcgccact
     7381 atatgcgttc acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc
     7441 tgagcgtcgc ccaggtcgaa gaaatgtatc gctatctcgc cattgccaac tatgaagatc
     7501 gttttgttat cccgaccagc catcgggaaa tggcgggcga tgccttcgca gaacgcaacg
     7561 gctgcggttt tacctttggc gacggttgcc acggttcgga cagtaaattc aacctgttca
     7621 acagtagccg tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat
     7681 aatgcagatc ctcaaagtga tcggcctgtt gatggagtat ccggacgagc tgttgtggga
     7741 atgcaaggag gacgcgctgg cgttgatccg ccgcgacgcg ccgatgctta cggatttcac
     7801 ccacaacctg cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg aagtgtttga
     7861 ccgcgggcgc accacgtcgc tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg
     7921 cggccaggca atggtggacc tgctggcgga gtatgaaaag gtcggcctgc agctggattg
     7981 tcgggaactg cccgattatc taccgctgta tctggagtat ttaagcatac tgcctgacga
     8041 tcaggcgaaa gaaggattgc tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt
     8101 aaaacaacgc aaggcaccgt ggtacgcgtt gtttgatgct ctgctgcaac tggcaggaag
     8161 tacactttca agtgacagtg tcacaaaaca agtaaacagc gaagagcgcg atgacacccg
     8221 ccaggcgctt gatgcagtgt gggaagagga acaggtgaag tttattgaag ataacgccac
     8281 ggcgtgtgac agctcgccgc ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc
     8341 gcagtatgtc gacatcagtg cgggaggtga gaaatgattc agtatctgaa cgtctttttt
     8401 tacgatatct acccgtacat ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac
     8461 gactacgggc agtacacctg gcgcgcctcc tcaagtcaga tgctcgataa acgcgggatg
     8521 gtgatatggt cgaatctgtt ccatatcggc attttgggga tcttcttcgg gcacctgttc
     8581 ggcatgttaa cgccgcactg gatgtacgcg tggtttttgc cagtggcagc gaaacaacta
     8641 atggcgatgg tgctcggtgg tatttgcggt gtgttgacgt tgattggcgg cgcaggactg
     8701 ctgtggcgca ggctgacaaa tcagcgcgtg cgtgccactt ccactacgcc ggatatcatc
     8761 atcatgagca ttttgctgat ccagtgtctg ttggggctaa gtaccatacc gttctcagca
     8821 cagtatcctg atggtagcga aatgatgaag ctggtgggtt gggcgcaaag cattgtcact
     8881 ttccgtggtg gatcgtcaga gatgcttaac ggtgtagcgt tcgtcttccg cctgcatctg
     8941 gtgttgggaa tgaccatctt cttgctcttc ccgttcaccc gactggtgca tgtgtggagc
     9001 gccccgtttg agtactttac tcgtcggtat cagattgtgc gctcgcggcg ataatttcac
     9061 cctggccgga tagcccttcg ctctccggcc acttgcaggc tatgatagtt ctccagtcac
     9121 aggagaacgt taatgaaacc gcaggtgtac cacgtcgatg cttttacctc acaaccgttt
     9181 cgcggcaatt ctgccggtgt tgttttcccc gccgataatc ttagcgaagc gcaaatgcag
     9241 cttatcgccc gcgagttagg ccattcggaa actgcttttc tgctgcacag cgacgacagc
     9301 gatgtgcgca tccgctactt tacgccaacg gttgaagtgc cgatttgcgg tcacgcgaca
     9361 gtagctgcgc actatgtacg tgcgaaggtg ttaggtttag gaaattgcac gatctggcaa
     9421 acatcgctgg caggaaaaca tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt
     9481 tcgctggaac aaggtacgcc gggctttgag ccaccgctgg aaggtgaaac acgtgaggcg
     9541 attatcaacg cgcttcatct cactgaagac gatattttgc cgggcttgcc aattcaggtc
     9601 gcaacaacag gacactcaaa agtgatgatc ccgttgaaac cggaagtgga tatcgacgcc
     9661 ctttcgcctg atcttgctgc gctgagcgct atcagcaaac agattggctg caatggtttc
     9721 ttcccgttcc agatccgccc aggcaaaaac gaaaccgatg gtcgtatgtt ctcgcctgcg
     9781 attggtattg tggaagatcc ggtgactggc aatgccaatg gcccgatggg tgcatggttg
     9841 gtacatcaca acgtattgcc ccacgatggc aacgtgttat gtgttaaagg ccatcagggg
     9901 cgcgcattgg ggcgtgacgg catgattgaa gtgacggtga caattcgtga taaccaaccg
     9961 gaaaaagtca ccatttcagg cacagcggtg attttgtttc atgccgaatg ggcaattgaa
    10021 ctctgaaacc gttgcctgat gcgcttcgcc tgtcaggtct acaaccgagc taatgtatgc
    10081 cggataaggc gtttacgccg catccggcaa cctgacataa attattttcc cgcctccggg
    10141 tgcgtatcaa acgccgccat caccagcgct aactcatcca cggtaaagcc atgtttcgca
    10201 tcatccacgc ccagaccaaa ctgttcttgc atcacagcat ataatgacgc cacatccggt
    10261 agatttcgct gctccaccgc gtgcccattt tcataatggg taaaatgaaa attggtcagc
    10321 gttagcttgc caccgtccgg caaatggcgg cacatcagca aatgatggcg aaaatgcgac
    10381 tgcggccagt gcgccgacca gaaattgccc atcacataat cgctttgttg ctgctcgcag
    10441 agatcaaaac ggtacatcga ctgccagtgc tgatgatgat taaactgcaa cacccaatca
    10501 tcaccctcct gcaacaaccg ataatctccg tgtggcgtgg tctgcacgag atcggaaact
    10561 aaacgaatcg gcgcggttag cgtctgccca ccgaaaccga catcagcaat ccatttttcc
    10621 tcttccagtt ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat
    10681 aacactacgc gccctaacaa gctgcgaacg ttaaactcca gctcgcgtaa cacccgctca
    10741 aacacgccat tctgctcaaa acagtaaccg ccacgacggg ctatcaccag tttctcttcc
    10801 agcgattgat catcaagctg tatttccctc ggcagcaaaa cgtcaaggtt ttcaaacgga
    10861 atggtgcaat tgtgtttcag gtgcaatgca cgaagcgtat caatattgac cgcagcagct
    10921 cccgaccagt taatacgggc aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct
    10981 tagtggtttt ctcaacttat aacctggaat agacgccgca ccggctattt tgtgcgatcg
    11041 cggacatgca tcacgtttgc aaatcgccaa tcccgttaat ttcgttactt attgcgttac
    11101 ggactacgct taaaaaaacc ttcttcgcga ggcgatacca tgagccgatt catccccata
    11161 gaattacacc atgccagccg tctattaaat catggcccaa ctgtcatgat caccagtttc
    11221 gatgagcaat cccagcggcg taacattatg gctgcagcct ggtcaatgcc ggtggagttt
    11281 gaaccgccac gcgtggcgat tgtggtagat aaatcgacat ggaccaggga gttgattgaa
    11341 cgtaacggta agtttggcat cgttatcccg ggcgttgcag caactaactg gacgtgggca
    11401 gtgggaagcg tgtcgggtcg tgaagaagat aaatttaatt gctatggcat tccggtggtg
    11461 aaaggtccgg tgcttggttt gccacttgtt gaagagaaat gcctggcgtg gatggagtgc
    11521 cgcctgctac ctgcgacttc tgcgcaacaa aaatacgaca cgctgtttgg cgaggtggta
    11581 tcagcagcgg cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag
    11641 ctcaatacgt tgcatcattt aggtgctggc acgtttgtta ctagcggcaa gcgtgttacg
    11701 gcaggttaag cattacatgc tgtaaccggg tttctttatc aaagcctcca tttggggcgc
    11761 gatttcggca tcccagatag cttgccaaga ttctggttga atctgctgta gagcaatgct
    11821 tatcgaactg tctttactgt tcagatggcg aataataacg tcggtaatat ctgcagcaag
    11881 tgctgctttt tgttgttcgt ccagttcacg cggaaagcat ttaatgtcga tgtgcggcat
    11941 gattacgctc cttttgaaag atgtcagtaa aattaacaca cattgtgaat gtcatgtgtc
    12001 cggtaaagcg tttatacgac aataatgcca gggcaagatt acgaaagcct tctccccgca
    12061 aggactgacg ccaggtagtt tctgtccatg gctgcttttc gcatcttacg tcttaaccct
    12121 gccttgaata ccttatcatt cgtcaaaata ttaatagcaa tgtgccgtat ccctgaaaat
    12181 aattctgctg catttccttt tcttattttg cagtcgtctt cattcattac cacgtccaga
    12241 cgccagtgca gcttattctc cacgtgccag tggtttcgga ttgctgtggc gaacttctct
    12301 gcggttaaat cagcagaact gatataatat ctgaccgtca tttctggctc tttcttttgt
    12361 tctgctatta tggaccgaaa ggagactgcc acgcataatt tcttcag
//
LOCUS       DAZFVO020000144        12393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFVO020000144 DAZFVO020000000
VERSION     DAZFVO020000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.145"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..91
                     /locus_tag="ACHKNB_RS19395"
                     /old_locus_tag="pgaptmp_003879"
     CDS             <1..91
                     /locus_tag="ACHKNB_RS19395"
                     /old_locus_tag="pgaptmp_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197304.1"
                     /translation="GHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(131..1351)
                     /gene="ydcO"
                     /locus_tag="ACHKNB_RS19400"
                     /old_locus_tag="pgaptmp_003880"
     CDS             complement(131..1351)
                     /gene="ydcO"
                     /locus_tag="ACHKNB_RS19400"
                     /old_locus_tag="pgaptmp_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HGU4197305.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGV
                     CYVVLNLIADRNRY"
     gene            1404..1940
                     /gene="sutR"
                     /locus_tag="ACHKNB_RS19405"
                     /old_locus_tag="pgaptmp_003881"
     CDS             1404..1940
                     /gene="sutR"
                     /locus_tag="ACHKNB_RS19405"
                     /old_locus_tag="pgaptmp_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HGU4197306.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2013..3974
                     /gene="rlhA"
                     /locus_tag="ACHKNB_RS19410"
                     /old_locus_tag="pgaptmp_003882"
     CDS             2013..3974
                     /gene="rlhA"
                     /locus_tag="ACHKNB_RS19410"
                     /old_locus_tag="pgaptmp_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HGU4197307.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLFKRKG"
     gene            complement(4066..4296)
                     /gene="yncJ"
                     /locus_tag="ACHKNB_RS19415"
                     /old_locus_tag="pgaptmp_003883"
     CDS             complement(4066..4296)
                     /gene="yncJ"
                     /locus_tag="ACHKNB_RS19415"
                     /old_locus_tag="pgaptmp_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HGU4197308.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4518..4694
                     /gene="hicA"
                     /locus_tag="ACHKNB_RS19420"
                     /old_locus_tag="pgaptmp_003884"
     CDS             4518..4694
                     /gene="hicA"
                     /locus_tag="ACHKNB_RS19420"
                     /old_locus_tag="pgaptmp_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HGU4197309.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4740..5156
                     /gene="hicB"
                     /locus_tag="ACHKNB_RS19425"
                     /old_locus_tag="pgaptmp_003885"
     CDS             4740..5156
                     /gene="hicB"
                     /locus_tag="ACHKNB_RS19425"
                     /old_locus_tag="pgaptmp_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HGU4197310.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5235..6641
                     /gene="ydcR"
                     /locus_tag="ACHKNB_RS19430"
                     /old_locus_tag="pgaptmp_003886"
     CDS             5235..6641
                     /gene="ydcR"
                     /locus_tag="ACHKNB_RS19430"
                     /old_locus_tag="pgaptmp_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGU4197311.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6886..8031
                     /gene="ydcS"
                     /locus_tag="ACHKNB_RS19435"
                     /old_locus_tag="pgaptmp_003887"
     CDS             6886..8031
                     /gene="ydcS"
                     /locus_tag="ACHKNB_RS19435"
                     /old_locus_tag="pgaptmp_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HGU4197312.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8049..9062
                     /gene="ydcT"
                     /locus_tag="ACHKNB_RS19440"
                     /old_locus_tag="pgaptmp_003888"
     CDS             8049..9062
                     /gene="ydcT"
                     /locus_tag="ACHKNB_RS19440"
                     /old_locus_tag="pgaptmp_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGU4197313.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9063..10003
                     /gene="ydcU"
                     /locus_tag="ACHKNB_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /pseudo
     CDS             9063..10003
                     /gene="ydcU"
                     /locus_tag="ACHKNB_RS19445"
                     /old_locus_tag="pgaptmp_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9993..10787
                     /gene="ydcV"
                     /locus_tag="ACHKNB_RS19450"
                     /old_locus_tag="pgaptmp_003890"
     CDS             9993..10787
                     /gene="ydcV"
                     /locus_tag="ACHKNB_RS19450"
                     /old_locus_tag="pgaptmp_003890"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4197314.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10809..12233
                     /gene="patD"
                     /locus_tag="ACHKNB_RS19455"
                     /old_locus_tag="pgaptmp_003891"
     CDS             10809..12233
                     /gene="patD"
                     /locus_tag="ACHKNB_RS19455"
                     /old_locus_tag="pgaptmp_003891"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HGU4197315.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAT
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2989 a         3039 c         3324 g         3041 t
ORIGIN      
        1 ggggcacgcc gttcttgcct gtggagagat ggtgcagtca ggccgcccgt tgaagcagga
       61 acccaccgaa atgattcagg cgacagcctg aacgtagcag ggatcctcgt ccttcagggc
      121 gtggaggatg tcaatatcgg tttctgtcag cgattaaatt caagaccacg taacaaacgc
      181 ctccggcaat taatccccag aacgcagaac caatcccgac cagcgtcaat ccacttgccg
      241 ttaccagaaa tgccaccacc gccgcgtcgc gctcacgctc acgctcatta tgcagcgcct
      301 gatacaaact gccgctaatg gtacttaaca gcgccagacc tgccagcatc tggatccaac
      361 ttacgggcag ggcagccatc atcccggtaa tagcactacc aaacagacct gcgatcaaat
      421 aaaaaatgcc tgcaacggca gctgccagcc aacgttgatc tttatccgga tgcgcttccg
      481 ggctttggca aatagccgcg gtgattgccg caataccgac ggaataaacg ccgaaagggg
      541 aaaaaaccag tgccagcaat ccagtaaata caattaatgg cgaaacagga gccgaatacc
      601 cggctgcttt cattgctgcg atacccggtg cgttttgcga tgccatcgtc accagaaaaa
      661 gggggagtgc aacgctcagg ctgtgagcaa acgaaaaatc aggggtaata taagtgggga
      721 gaacgggttt aaagacaaca tcagttgtga caacgtcacc ttgcgcgata acgatcacga
      781 tcccaataat catcgcggca attaccgcat agcgcggcgc aacggccttg gttgccagcc
      841 ataccagcaa catacttcca cacaacgtaa attgaccgtc cagactggca aacgcctgta
      901 aaccaaagcg taataaaatc ccggcaagca ttgccgccgc aagcgagtgc ggaataatgc
      961 gcatcagacg agcaaagagt cccgttatgc cgcagaggac tattaacgcg ttggtgacaa
     1021 taaaaacgcc gatggcttcg ttaagtgtta gtccctgcaa tccggtgacc aacaaagccg
     1081 cgccaggcgt tgaccatgcg gtgagaacag gtacgcgata ccataatgtc agagtcagcg
     1141 tactgacgcc cattgccagc cccagcgccg tcatccagcc agagatttgt gcagtggtgg
     1201 ctccggcgac aatcgctgct tgccagatta ttgccgctga actggcgtag ccaattaata
     1261 ccgccagaaa ccccgccagt agcgtgggtg gaggaataga aaacagacgc atagtcacct
     1321 cgtgcgttat agcgtacagg caccgtagca tttgtccgtt ataacgcaca agtgataaac
     1381 ttccgttttg ccggaggagt cgcatggaaa atctcgctcg ctttttatcc accacactta
     1441 aacaactacg ccagcagcgc ggctggagtc tttcgcgact ggcagaagcg acgggcgttt
     1501 ctaaagcaat gttagggcag attgagcgta atgagtccag tccgacggtc gcgacattat
     1561 ggaaaattgc caccgggttg aacgtaccat tttcaacatt tatttctccg ccgcagtccg
     1621 ctacgccttc agtttatgat ccacaacagc aggcgatggt gatcacttcg ctgtttcctt
     1681 atgatccaca actctgcttt gaacacttct cgattcagat ggcatctggt gcaataagcg
     1741 aatcgacgcc ccatgagaag ggggtaatcg aacatgtcgt cgtcattgat ggacaacttg
     1801 atctgtgcgt tgatggggaa tggcagaccc ttaattgtgg ggaaggcgtt cgatttgctg
     1861 cagacgtcac gcatatctat cgtaatggtg gggagcaaac cgtacatttt cattccctca
     1921 tccattaccc gcgcagttaa gcgggaaaac tatttcgcaa cgtcgcgctt gtggctaaaa
     1981 tagccgccat ttttcagcta ctggataaga atgtgaccgt atcttctcat cgacttgaac
     2041 tgttaagccc ggcacgcgat gccgccattg cccgcgaagc tattttgcac ggtgccgatg
     2101 ctgtttatat cggcggccct ggttttggtg cccgtcataa tgccagtaat agcctgaaag
     2161 atattgccga gctggtgccg tttgcccatc gttatggtgc aaaaattttc gtcacgctta
     2221 acaccatttt gcatgatgat gagctggaac ccgcgcaacg gctgattact gacctctacc
     2281 agaccggtgt cgatgcgctg attgttcagg atatggggat tctggaactt gatattccgc
     2341 cgattgaact gcacgccagt acgcagtgcg acattcgtac agttgaaaaa gcgaagttcc
     2401 tctctgatgt tggcttcacg cagattgtgc tggcgcgaga gctgaatctt gatcagatcc
     2461 gcgcgattca ccaggctacg gacgcgacca ttgaattctt tattcatggg gcactgtgcg
     2521 tggcctattc gggtcagtgc tacatttctc atgcgcaaac agggcgtagc gccaaccgtg
     2581 gcgattgctc gcaggcgtgc cgtttgccat acacattgaa agacgatcag gggcgggtgg
     2641 tttcctatga aaaacatctg ctgtcgatga aagataacga tcagactgcc aacctcggcg
     2701 cgctgattga tgctggtgta cgctccttca agattgaagg gcgttacaaa gatatgagct
     2761 acgtgaagaa tatcaccgcc cattatcgcc agatgcttga tgccattatt gaagaacgtg
     2821 gcgatctggc gcgcgcttca tcaggtcgta ctgaacattt ctttgttcca tcgacggaaa
     2881 agactttcca ccgtggtagc acagattatt ttgtgaatgc ccgtaaaggc gatattggcg
     2941 cgttcgattc gccgaaattt atcggcctgc cggtaggcga agtattgaaa gtggcgaaag
     3001 atcatctcga tgttgccgtt accgagccac tggcaaatgg cgatggcctg aacgtgttga
     3061 ttaaacgtga agtcgtcggt tttcgtgcca atacggtcga gaaaaccgga gaaaatcagt
     3121 accgcgtctg gcccaatgaa atgccagcag atttgcacaa aattcgtcca catcacccac
     3181 taaaccgtaa tcttgatcat aactggcagc aggcactgac aaaaacctcc agcgaacgtc
     3241 gggtggcggt agacattgaa ctgggcggct ggcaggaaca actgattctg accctcacca
     3301 gtgaagaggg tgtcagcatc acgcatacgc tggacgggca gttcgacgaa gccaataacg
     3361 ccgaaaaagc aatgaacaat ctgaaggatg gtctggcaaa actggggcaa accctctatt
     3421 acgcccgcga tgtgcaaatt aatttgccgg gggcgctgtt tgtaccaaac agtctgttaa
     3481 accagttccg ccgtgaagct gctgacatgc tggatgctgc gcgtcttgcc agttaccagc
     3541 gcggcagccg taaaccggtt gctgatcctg cgccggttta tccgcaaacg catctgagtt
     3601 tcctcgcgaa cgtatacaac cagaaagcgc gtgaatttta tcatcgctat ggtgtgcagc
     3661 tgattgacgc ggcgtatgaa gcacatgaag agaagggcga agtcccggtg atgatcacca
     3721 agcattgtct gcgctttgcc tttaatctgt gcccgaaaca ggcgaaaggc aatatcaaaa
     3781 gctggaaggc gacgccaatg caactggtta acggcgatga agtattaacg ctaaagtttg
     3841 attgccgccc atgcgagatg cacgtcattg gcaaaatcaa aaatcacata ctgaaaatgc
     3901 cgttaccggg aagcgtagtg gcatccgtaa gtccggatga gctgctgaaa acattgttta
     3961 agcgaaaagg gtaaaacgcc agttttctgg ttactcacaa ctcattgaat ctgcatgata
     4021 ttgcctgccg ggtaaggcgt ctcgccgcat ccggcaacaa atgactcaac gttgtcccgc
     4081 ttctggtttg cgcgattttt gccagtaaaa atgctcgcgc aaaccttccg ccgactcttc
     4141 cgccacagca cgcaattcat cgctgtccgc ttcatgacgc agctgatgat ccacattctt
     4201 tacccacaca aattcatgtc ctttgtgccc tgccatgagt tgtcctgaga acagcgcaca
     4261 cgttaataag acaaccgata acgccttcgt aaacatcctg ccaccttttt cttacctttt
     4321 gccgctatga tgccgatcgt ttcttgaggt tattattcag ttttgcaaat tagcgcaaag
     4381 aaattctgga atcttccttc ctgattttgc atggcattct gccgttgcgg cgatttagtg
     4441 ctattttcga gcaaattaca cacggaggta aacaataatg tttactttgt tgatatactc
     4501 agcggcaggg aggcgatgtg aaacaaagcg agttcagacg ttggctcgaa tctcagggcg
     4561 tcgatgtagc gaatggcagc aaccatttga aactcaggtt tcatgggagg cgcagtgtca
     4621 tgccgcgtca cccctgcgat gagattaaag aaccattgcg taaagcaatc ctgaaacaac
     4681 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagggaca gtcgaaatta
     4741 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4801 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4861 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc cctttacctt
     4921 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaggtat
     4981 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     5041 gcaaacctaa acaggagatt actcgcctat ttaacttgca tcatgcgaca aaaatcgacg
     5101 ccgtccagct cgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     5161 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     5221 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     5281 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     5341 ctggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt ctgggatata
     5401 ttatcgcgcg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5461 cgccagtcat tccagtcact cgagatgaag cagtcgatat caacacttat atttttgata
     5521 tgttgcaggc cagtcgcgat ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5581 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5641 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5701 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5761 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5821 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5881 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5941 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     6001 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     6061 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     6121 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tttctggtgc
     6181 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     6241 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     6301 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     6361 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6421 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6481 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6541 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6601 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6661 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6721 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6781 tctcaggggc gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6841 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6901 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6961 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     7021 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     7081 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     7141 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     7201 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     7261 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     7321 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     7381 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7441 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7501 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7561 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7621 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7681 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7741 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7801 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7861 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7921 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7981 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     8041 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtac
     8101 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     8161 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     8221 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     8281 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     8341 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     8401 cgctggagaa agtggcgttg gggtttgtac atcaacgtaa accgtcacaa ctttctggtg
     8461 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8521 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8581 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8641 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8701 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccggattc gtcgggacat
     8761 ccaacgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8821 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8881 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8941 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     9001 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     9061 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctgggtaag gtgtccggtt
     9121 tcttctggcg taatccgggg ctggggctgt ttttactgct gcttggcccg ctaatgtggt
     9181 ttggcattgt ctatttcggc tcgctgctga cactgttatg gcagggattt tatacttttg
     9241 acgatttcac catgtcggta acgccggaac tgacgctggc gaatatccgt gcgctgttta
     9301 atccggcgaa ttacgacatc attctccgca cgctgactat ggctgtggca gtcactatcg
     9361 ccagcgccat tctggctttt ccaatggcgt ggtatatggc gcgctatacc agcgggaaaa
     9421 tgaaagcttt tttttatatt gcggtaatgt tgccgatgtg ggcgagctac attgttaaag
     9481 cctatgcctg gacgttattg ctggcaaaag atggcgtggc tcagtggttt ttacaacatc
     9541 ttgggctgga accactgctg ctgcgttcct tacattacct gcggtgggcg gaaatacgct
     9601 gtcaacttcc gggctggggc gctttctggt gtttctctat atctggttgc cgttcatgat
     9661 cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca ttgttgcagg cgtcggctga
     9721 tctcggcgca cgtccacgac aaacctttcg ctatgtggtg ctgccgctgg caatcccggg
     9781 tattgccgct ggctctatct ttaccttctc actcacactg ggcgatttta tcgtcccgca
     9841 gctggttggt cctccaggat attttatcgg caatatggtt tattcccagc agggggcgat
     9901 tggcaatatg ccgatggcgg cggcattcac cctggtgccg attgttctca tcgcactgta
     9961 cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc
    10021 tcaaactggc ggcctggggc ggcgtggttt tcctacattt tcccatcctg attatcgccg
    10081 cttatgcgtt taacactgaa gatgcggcgt ttagttttcc accgcagggc ctgacgctgc
    10141 gctggtttag cgtggcagca cagcgtagtg atattcttga tgccgtgaca ctgtcactta
    10201 aagtggcggc gctggcgaca ttaatggcgc tggtgttagg gacgctggca gctgccgcgc
    10261 tgtggcgacg agactttttc ggcaaaaacg ccatttcgct gttactgctg ctgcccattg
    10321 cgctgccggg cattgtcact ggtctggcgc tattaaccgc ttttaaaacc atcaatctgg
    10381 agccgggatt tttcaccatc gtggtcggtc atgcgacttt ttgcgtagtg gtggtgttta
    10441 acaatgtcat cgcccgtttt cgccgcacct cgtggagtct ggttgaggcg tcaatggatc
    10501 ttggggccaa tggctggcaa accttccgct acgtagtgtt gccgaatctc agttcggcgc
    10561 tactggcagg aggaatgctg gcgtttgcct tgtcgttcga tgaaatcatc gttacgactt
    10621 ttacggcagg tcatgaacga acgttaccgt tgtggttgct caatcagctt gggcgaccgc
    10681 gtgatgtacc ggtaactaac gtggtggcac tgctggttat gttggtaaca accttgccga
    10741 tcctgggggc ctggtggcta acccgcgaag gcgacaatgg tcaataacca ctgatacagg
    10801 aatatgctat gcaacataag ttactgatta acggagaact ggttagcggc gaaggggaaa
    10861 aacagcctgt ctataatccg gcaacggggg acgttttact ggagattgcc gaggcatccg
    10921 cagagcaggt cgatgctact gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa
    10981 ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg
    11041 gtcaggtttt tgccgaactg gagtcccgta attgtggcaa accgctgcat agtgcgttca
    11101 atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc
    11161 tgaatggtct ggcggcaggt gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc
    11221 cgttgggggt cgtggcttct atcgcaccgt ggaattatcc gctgatgatg gcagcgtgga
    11281 aactggctcc ggcgctggcg gcagggaact gcgtagtgct taaaccatca gaaattaccc
    11341 cgctgaccgc gttgaagttg gcagagctgg cgaaagatat cttcccagca ggcgtgatta
    11401 acgtactgtt tggcagaggc aaaacggtgg gtgatccgct gaccggtcat cccaaagtgc
    11461 ggatggtgtc gctgacgggc tctatcgcca ccggcgagca catcatcagc cataccgcgt
    11521 cgtccattaa gcgtactcat atggaacttg gtggcaaagc gccagtgatt gtttttgatg
    11581 atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt tggctattac aatgctggac
    11641 aggattgtac tgcggcttgt cggatctacg cgcaaaaagg catttacgat acgctggtgg
    11701 aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc gccggatgac gagtctacgg
    11761 agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg
    11821 cgaaagcgac agggcacatc aaagtgatca ctggcggtga aaagcgcaag ggtaatggct
    11881 attactatgc gccgacgctg ctggctggcg cattacagga cgatgccatc gtgcaaaaag
    11941 aggtatttgg tccagtagtg agtgttacgc ccttcgacaa cgaagaacag gtggtgaact
    12001 gggcgaatga cagccagtac ggacttgcat cttcggtatg gacgaaagat gtgggcaggg
    12061 cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg ggtcaatacc catttcatgc
    12121 tggtaagtga aatgccgcac ggtgggcaga aactttctgg ttacggcaag gatatgtcac
    12181 tttatgggct ggaggattac accgtcgtcc gccacgtcat ggttaaacat taagttagat
    12241 gttagtggct gttataggac ggccactatc gttttaggtt ctgagtttaa tggcggttaa
    12301 gtattagaat gattacatta ataactagcg taaaaattag gctaaatgtt tgatggcgat
    12361 ttttcatcct gtcacagttg attagcataa ggc
//
LOCUS       DAZFVO020000145        12196 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFVO020000145 DAZFVO020000000
VERSION     DAZFVO020000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 12196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.146"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(39..947)
                     /gene="galU"
                     /locus_tag="ACHKNB_RS19460"
                     /old_locus_tag="pgaptmp_003892"
     CDS             complement(39..947)
                     /gene="galU"
                     /locus_tag="ACHKNB_RS19460"
                     /old_locus_tag="pgaptmp_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGU4197316.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1149..2162)
                     /gene="rssB"
                     /locus_tag="ACHKNB_RS19465"
                     /old_locus_tag="pgaptmp_003893"
     CDS             complement(1149..2162)
                     /gene="rssB"
                     /locus_tag="ACHKNB_RS19465"
                     /old_locus_tag="pgaptmp_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HGU4197317.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2254..3159)
                     /gene="rssA"
                     /locus_tag="ACHKNB_RS19470"
                     /old_locus_tag="pgaptmp_003894"
     CDS             complement(2254..3159)
                     /gene="rssA"
                     /locus_tag="ACHKNB_RS19470"
                     /old_locus_tag="pgaptmp_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HGU4197318.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3272..3730
                     /locus_tag="ACHKNB_RS19475"
                     /old_locus_tag="pgaptmp_003895"
     CDS             3272..3730
                     /locus_tag="ACHKNB_RS19475"
                     /old_locus_tag="pgaptmp_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HGU4197319.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFENCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3780..4622
                     /gene="purU"
                     /locus_tag="ACHKNB_RS19480"
                     /old_locus_tag="pgaptmp_003896"
     CDS             3780..4622
                     /gene="purU"
                     /locus_tag="ACHKNB_RS19480"
                     /old_locus_tag="pgaptmp_003896"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HGU4197320.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4782..4866
                     /locus_tag="ACHKNB_RS19485"
                     /old_locus_tag="pgaptmp_003897"
     tRNA            4782..4866
                     /locus_tag="ACHKNB_RS19485"
                     /old_locus_tag="pgaptmp_003897"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4816..4818,aa:Tyr,seq:gta)
     gene            4901..4985
                     /locus_tag="ACHKNB_RS19490"
                     /old_locus_tag="pgaptmp_003898"
     tRNA            4901..4985
                     /locus_tag="ACHKNB_RS19490"
                     /old_locus_tag="pgaptmp_003898"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4935..4937,aa:Tyr,seq:gta)
     gene            5033..5164
                     /locus_tag="ACHKNB_RS19495"
                     /old_locus_tag="pgaptmp_003899"
     ncRNA           5033..5164
                     /ncRNA_class="other"
                     /locus_tag="ACHKNB_RS19495"
                     /old_locus_tag="pgaptmp_003899"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5348..6025)
                     /gene="narI"
                     /locus_tag="ACHKNB_RS19500"
                     /old_locus_tag="pgaptmp_003900"
     CDS             complement(5348..6025)
                     /gene="narI"
                     /locus_tag="ACHKNB_RS19500"
                     /old_locus_tag="pgaptmp_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HGU4197321.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(6025..6735)
                     /gene="narJ"
                     /locus_tag="ACHKNB_RS19505"
                     /old_locus_tag="pgaptmp_003901"
     CDS             complement(6025..6735)
                     /gene="narJ"
                     /locus_tag="ACHKNB_RS19505"
                     /old_locus_tag="pgaptmp_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HGU4197322.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6732..8270)
                     /gene="narH"
                     /locus_tag="ACHKNB_RS19510"
                     /old_locus_tag="pgaptmp_003902"
     CDS             complement(6732..8270)
                     /gene="narH"
                     /locus_tag="ACHKNB_RS19510"
                     /old_locus_tag="pgaptmp_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HGU4197323.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8267..12010)
                     /locus_tag="ACHKNB_RS19515"
                     /old_locus_tag="pgaptmp_003903"
     CDS             complement(8267..12010)
                     /locus_tag="ACHKNB_RS19515"
                     /old_locus_tag="pgaptmp_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HGU4197324.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVESLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2896 a         3321 c         3000 g         2979 t
ORIGIN      
        1 tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt acttcttaat gcccatctct
       61 tcttcaagcc aggctttaaa ttccgtgcca agggtgttat gacgaatacc gtattcaacg
      121 aaggcctgca tgtaacctaa tttattaccg cagtcatggc tcttcccttt catatgatag
      181 gcttccaccg tttctttttc gatcagcata tcaattgcgt cggtgagctg aatttcatca
      241 ccagctcccg gaggggtttt tgccagcaac ggccaaatat ccgcgctaag tacgtaacga
      301 cccacaatag cgagattaga cggcgcaaca tccgctttcg gtttttctac cacaccaacc
      361 atcggtacgc tttcaccagg cgctaattca acgcctttgc aatccacaac gccatatgcg
      421 gtcacatcag caaccggttc aaccatgatc tggctatgac ccgtttcatc aaagcggcgg
      481 atcatctctg ccaggttatc ctgtgacaaa tcggattcat attcatccag aataacatca
      541 ggcaaaataa cagctaccgg ttcatcaccc actaccgggt gagcacacaa taccgcgtgt
      601 cccaggcctt tcgccagacc ctgacgaact tgcataatag tcacgtgcgg tggacaaata
      661 gactgcactt catcaagcag ttgacgtttt acacgttttt ccagcattgc ttccagttca
      721 aaactggtat caaagtggtt ttcaatagag tttttagatg agtgtgtaac cagcacaatt
      781 tcagtaatgc cagccgcaat acattcattc acgacgtatt gaattaatgg cttatcgaca
      841 agtggcagca tctctttcgg gatggctttc gtcgccggca acatcctggt tcctaatccc
      901 gcaacgggga taacggcttt tttgactttc gtattaatgg cagccattta aattctcctg
      961 gactgttcgt gttttgaacg tgttcatatt tctgtatcgc atcccagtat atcagcaccc
     1021 cgaaggggct aagttctgaa aaggaggcga gttagcataa ttaagacaat tacttatacg
     1081 aatggcgacg atagtaccac cagccgacat tagcaggtaa tgcaaattta gcccgcgtta
     1141 tcgtttgctc attctgcaga caacatcaag cgcagtcgac caccggttcc ccatatttgg
     1201 cattgccagg catcgcatcg ctggctcaat tgattcaaat aagcgttacc taaagtgcct
     1261 aacggaacac cattactgat ttgcacctgg tgttcgccgg tatttaacgt cgcattcaga
     1321 cccgcagaaa ccagaatgag atttttcagt tcgcgatgat aatagccaac taatagcgga
     1381 aactgccccg gcagattggc ctgacgaagt aaatggttta cctgcttcaa taacgcgccc
     1441 aactctggca accgttgatt ttggtgtgca agctgttcct gtaataatcc gttaaacaat
     1501 gcgcgtaata acaaggcagc aagtacgcca ttatgtccag ctcgggtgac atcaaggcaa
     1561 taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa gcaccaggcc gggtttgtcc
     1621 gcggcaacca attgacgata attaacccgg caatgggaaa tcacctgctg aaccggcggt
     1681 tgtagttcct gtaataattt cgccgctgcg gcagggttat caaccattgc atcccagtcg
     1741 cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca tgctgggata gagacaggca
     1801 aaaaccatct cgcgcaagcg attcagatct ttaactggtt tcagcaaaac atcttcaacg
     1861 cccagacgta acgctttggc aatatctgcc atattttcag tggcagatat caccagaacc
     1921 ggggtctggt cgcctctgtt acgtatatgc tccagcagtt taagcccgtt cattcgtggc
     1981 atcgcgatat cacatatcat caggtctgga gtgaaacctc ccagcaactc aagggcatcc
     2041 accccatcag ccgccagtac cgttgtcgct cccaatgagg aaaaccatga atccagaagc
     2101 gagcgaaata cctgctcatc ttcaacaatg agaatctgtt ttccgaccaa tggctgcgtc
     2161 atgttctctc ccctgactgg ctttactcaa tagtggcatg ctattgccac ctgcgcctgt
     2221 cagaattaac ttaagtgtag ataaaaattc tggtcaaatg ttggtgcgta ccaacggcaa
     2281 aagttcgtcc attttccttt ccactgccag ctgtccggcc gcaatggcag cgtgcgcgcg
     2341 atggaaatca agcgtagata tttgcgggca aacaggttga atcagaatat cgggcggatc
     2401 acctgccatg cggttccttt taaggcggtt ctccagcacc tggatagaag tggtcataat
     2461 ctctgttgcc gttggtgctg tcaccgcccg acgtgtcgtt atgctgccta acctttcttt
     2521 cagacgcgca tgccacggca gagaatcacc attctcgcta ttttcttcac tgacattaaa
     2581 ggagagcaaa tcttgttgca ttaaatgagc atcgtgctgc aggtcgaccg ctatcacaat
     2641 atcagcaccc aatgcacgcg tgagcgaaat aggaattggg ttaacgactg ccccatcaac
     2701 cagccagtag ccgttatgcg cgactggtgc catgagtcct ggaatactgc atgacgcgcg
     2761 aatagcaaga tggagatcgc cttcagtaaa ccataattca cgcccagtac ttaaattggt
     2821 ggcaacagcc gcgaagcgac gggaacaatt ttcgatctct gtttccggca ttatctcgcg
     2881 gtattgattg aagacacgct cgccgcgcag taacccaccg cgctgccagg agagatccat
     2941 caggcgtaaa acatcccaat aactgaaaga ggttacccaa tcttccagcg cagataatcg
     3001 atcgcatgca taggccgcgc ccaccagcga accaattgaa catcctgcaa cgatatcaat
     3061 ttcaataccc acttttttta gcgcattaat aacgccaata tgcgaccaac ctctcgccgc
     3121 gccagatccc agcgccagcc ctatttttat ctttctcatt attcccgcaa gattcccctg
     3181 gaatgcaatc gtagccacat cgctactgct cggttaacat agtgctactc aggcggttaa
     3241 tacgccgtta ttgtttccag ggagattatt tgtgtctcag ctttgtccct gtggtagtgc
     3301 tgtcgagtat agcctatgtt gccaccctta tgtgtctggt gaaaaggttg cacctgatcc
     3361 agaacatctc atgcgttcgc gttactgcgc ttttgtgatg caagacgcag attatttaat
     3421 aaagacctgg catccatctt gtggagcagc ggcgttacgt gccgaattga ttgccggatt
     3481 tgcgcatact gaatggttgg gattgaccgt atttgaaaat tgttggcagg acgccgacaa
     3541 tatcggattt gtcagttttg ttgctcgatt taccgaaggt ggtaaaacag gtgcaataat
     3601 tgaacgttcg cgatttttga aagaaaacgg tcaatggtac tatattgacg gtacacgtcc
     3661 gcagtttggt cgcaacgatc cctgcccttg tggttcaggt aaaaaattta aaaagtgctg
     3721 cggccaataa tggttgacgg tacggtttag caaacactct caacaaggtt tttccagcaa
     3781 tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc ggaccaaaaa ggtctgatcg
     3841 cacgcattac caatatttgc tacaagcacg agttaaatat cgtacagaac aatgaatttg
     3901 ttgatcaccg taccgggcgc ttttttatgc gcacggaact ggaagggatt tttaatgatt
     3961 ccacccttct ggcggatctc gatagcgcat tgccagaagg ctccgtgcgt gagctgaatc
     4021 ctgccggtcg tcgccggata gtgattctgg tcactaaaga agcgcattgc cttggcgatt
     4081 tgttgatgaa agccaattac ggcggcctgg atgtcgaaat cgcggcagtt attggtaacc
     4141 acgatacttt acgttctctg gttgagcgtt ttgatattcc gtttgagctg gtaagccatg
     4201 aagggttaag ccgcaacgag cacgatcaaa agatggcgga cgccattgat gcttatcaac
     4261 ctgactatgt ggtgctggcg aagtatatgc gggtattaac gccggaattt gtggcacgct
     4321 tcccgaataa aatcatcaat attcaccatt cattcctgcc tgcgtttatc ggcgcacgtc
     4381 cttatcacca ggcctatgaa cgtggtgtga agattattgg cgcaaccgct cactatgtga
     4441 atgacaatct ggacgaaggc ccaatcatca tgcaggacgt tattcatgtc gatcatacct
     4501 acacagctga agatatgatg cgcgcaggtc gtgacgtcga gaaaaacgtc ttaagtcgtg
     4561 cgctatacaa agtactggca cagcgcgtct ttgtttacgg taatcggacg attattcttt
     4621 aatcgccagc aaaaataact ggttaccttt aatccgttac ggatgaaaat tacgcaacca
     4681 gttcattttt ctcaacgtaa cactttacag cggcgcgtca tttgatatga tgcgccccgc
     4741 ttcccgataa gggagcaggc cagtaaaaag cattaccccg tggtggggtt cccgagcggc
     4801 caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttcctcca
     4861 ccaccatctc aagtattacc tcaaaatttg aattacccct ggtggggttc ccgagcggcc
     4921 aaagggagca gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc cttcccccac
     4981 caccatcact tttcaaaagt ccgtaaactc tcaaacgaat ccgcaattaa atattctgcc
     5041 caagcgggga aggatgagaa gcttcgacca aggttcgact tgagcgccag cgagagagcg
     5101 ttgccgcagg caacgacccg aagggcgaag cgcgcagcgc tgagtaatcc ttcccccacc
     5161 accatcgctt ccttaaataa tcaaaacctg ttactccgag ctaattttgc ccgatggcac
     5221 cacgtttacc gggcctacaa tatcctcccc tgtaggtcgg ataagcgaag cgcatccgac
     5281 aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa gcagggtcta tgtgaactaa
     5341 aattcgctta gtgacgagcg cgcaccagct ggtacttacg tgtcagatac tccaccggta
     5401 cgctccagat gtgtaccaga cgcgagaacg ggaacagcag gaataacgtc atccccagca
     5461 ccaggtgcag acggaagata aacgccacac catcgaggtg ttgagaagcg ccaccgtgga
     5521 aggtcaccac cgactgcgcc cagccaacca gtttcatcat ctcgctaccg tccatatgct
     5581 gagcggagaa cggaatggtc aacaggccca gcgcgcactg gataacgagc agcgacagga
     5641 tcaggatatc cgctccggta gtggttgcac gcacgcgtgg gctgaacaga cgacgcttca
     5701 gcaacagcac gccgccaatc agacacagca cgccgctggc accaccagca aacatcgcca
     5761 ttttctgttt cacttcaatc ggcagccagg cttcatacat ccagtgcggc gtcagcatac
     5821 cgaagaagtg accaacaaaa atccccagaa taccgatatg gaacaggttc gacgccaggt
     5881 tcatcccttt gcgatccagc atctggctgg acgccgcgcg ccaggtgtac tgcccgtagt
     5941 cataacgcag ccagctacca atcaggaaga ccgccccggc tatgtacggg tagatatcaa
     6001 agaagaacat attcaggaat tgcattagtg ctgtcctccg gtggtgatat tcagatattg
     6061 cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg attgctgaat caccgcagcc
     6121 tttgtcagca aagaatttaa cctgctcttc ttcccagaca gcatccagcg cctgcggcgt
     6181 atcatcgcgc gcttcgtcgg caattttttc cgccactttg tcgctgtcga tagcggtatt
     6241 ggccaatttc agcagcagat caaacatcac ggcataacgg ctttcacgct gttgcagacg
     6301 cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa ccttccacgg cttcgctttg
     6361 cggcagctgc gacaggtact ccagatacag cggcagatgg tccggtaatt cgcggctgtt
     6421 taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc accatcgcct ggccgcggtc
     6481 acgggattca ccgtgaacat gttcaaatag caacagtgac gtggcgcggc cacggtcgaa
     6541 cagttcgctg tactgggcct gggcatcgag cggatccatc gccgttaaat cgcgcaggaa
     6601 aatgcccagc gcatgggcat cttctttcga cagatttttc gacgcagcaa tcgcctcaaa
     6661 catctcttgt tgatgctgcc ataaggcagc atccggatat tcaaggagac gcgatacaat
     6721 cacgagttcg atcatggatg cggctccgtt ttgctggtca catcgatggc atcgatacga
     6781 cggctgttga acagattgaa tttggtatct gaaccgtggc agccatcacc aaaggtaaag
     6841 ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt cacgatgact actcggcacc
     6901 acaaagcgat cttcgtagtt agcaatcgcc agataacggt acatctcctg tgcctgggct
     6961 tcggtcagac cgacctcttc cagcgcacgg gtatcaactt taccgtcaac ggtttcagca
     7021 cgtttgtaat gacgcatcgc cagcatacgt ttcagtgcgc gcagtaccgg tttggtatca
     7081 ccggcggtca gcagattcgc cagatactgt accggaatac gcaagctttc gacgtctggc
     7141 agaatgccgt tgctacccag ctcacccgcg tctgctgcag actgaatcgg agacagaggc
     7201 ggcacgtacc agaccatcgg cagtgtgcga tattctggat gcagcggcag cgccagtttc
     7261 cattccattg ccattttata aaccggcgac tgctgtgcgg cttcaataac gctcagcgga
     7321 ataccgtctt taatcgcctg ctcgatgact ttcggatcgt tcggatcgag gaacacctcc
     7381 agctgacgct ggtaaagatc tttctcgttc tcggtgctgg ctgcacgttc aatcgcgtcg
     7441 gcatcgtaca gcagcacgcc aagataacgg atacgaccga cacaggtttc tgagcacacg
     7501 gtcggctgac ccgcttcaat acgcggatag cagaagatgc acttctcaga cttaccgctc
     7561 ttccagttga agtagatttt tttgtacggg catccggtga tgcacatacg ccagccacgg
     7621 catttatcct ggtcgatcag gacgatgcca tcttcttcac gcttgtaaat cgcaccgctc
     7681 gggcaggtcg caacacatgc cgggttcagg cagtgttcgc acaggcgcgg caaatacatc
     7741 atgaaggtgt tttcgaactg gctatacatc gccttctgga tgttgtcgaa gttcttgtct
     7801 ttcgccagtt tgtcaaactc accgcccagg tcatcttccc agttcggccc tttttcgatt
     7861 ttcgccatcc gttccccggt aatcagcgaa cgcggacggg caatcggctg cgatttgctg
     7921 ccttccggcg cggtatgcag gttctgatag tcaaaatcga acggctcgta ataatcgtcg
     7981 atccccggca gatgcgggtt agcgaagatt ttacccagca acatggcacg gttacccatg
     8041 cgcggctgca gtttgccgtt gattttacgg atccagccgc ctttgtattt ttcctggttt
     8101 tcccagtcag tcgggaagcc ctgacccggc ttggtttcca cgttgttgaa ccacgcgtat
     8161 tccacgcctt cacggctggt ccagacgttt ttacaggtaa ctgaacaggt gtggcagccg
     8221 atgcacttat cgagattcag caccatgccg acttgtgaac gaattttcat tttacgctct
     8281 cctgtacctg gtcattgcct tcgccatcta accagtcaat gttcttcatc ttacgcacta
     8341 caacaaactc atcgcggtta gaacctacgg tgccgtagta gttaaagccg tatgccagat
     8401 gggcatagcc gccgatcata tgcgtcggtt tcggcgtgat acgggtgacc gagttatgga
     8461 taccaccacg ctgttgggta atttccgaac caggcaggtt aacgatacgt tcctgcgcgt
     8521 ggtacatcat ggtcatccct gccggaacac gctggctgac aaccgcacgg gcagtcagag
     8581 caccgttgct gttgaagact tcaatccagt cgttatcggc gatacccaga tctttggcat
     8641 cggcttcact caaccagacc accggaccac cgcgacctaa agtcagcatc agcaggttgt
     8701 cgctgtaggt ggagtggata ccccacttct ggtgcggcgt caggaagttg agcgcttttt
     8761 ccgggttgcc gttggatttc tggcccatca cttctttcac cgaacgggtg tcgatcggcg
     8821 gacgataaac cagcaggctt tcaccgaaat cacgcatcca ttggtgatcc tgatacagct
     8881 gctgacggcc agagagcgta cgccacggga tcagctcgtg aacgttggtg taaccggcgt
     8941 tgtaagaaac gtgttcatct tcaaggccag accaggtcgg gctggagatg attttgcgcg
     9001 gctgtgcctg aatatcgcgg aagcgaatct tctcgtcttc tttattcagc gccagatgcg
     9061 tatggtcacg accggtaaac tcgctcaggg cagcccaggc tttcacggct acctgaccgt
     9121 tggtttccgg tgccagcgtc aggatcatct ctgccgcatc aattgcggtg ttcagcatcg
     9181 gctggccttt cgccggacct tccgctttgg tgtagttgag cttacgcagc agatccatct
     9241 cgctctgggt gttccaggca atccctttac cgccattacc gattttctcc atcagcgggc
     9301 cgatagaggt aaagcgttcg taagtcgccg gataatcgcg ctctacgacc ataatgtgtg
     9361 gcgcggtttt accagggatc aggtcgcact cgcctttttt ccagtctttc acatccagcg
     9421 gctgcgccag ttcagcggca gagtcgtgct ggataggcag cgtgacgatg tcggtttctt
     9481 tacccagatg accgacgcac acttcggaga atttcttcgc gatggctttg taaatttccc
     9541 agtcgctttt cgcttcccag gccggatcga ccgccgcaga cagcgggtga ataaacggat
     9601 gcatatccga agtattcatg tcgtcttttt cgtaccaggt cgccgtcggc aaaatgatgt
     9661 cggaatagag acaggtgctc gacagacgga agtccagcgt aaccaccaga tccagcttgc
     9721 cttccagacc attgtcctgc cagtccactt cttccggctt cacgccgccc tgttgcccca
     9781 gatctttccc ctggataccg tgctccgtcc ccagcaggta cttgagcata aactcatgac
     9841 ctttaccgga agaaccgagc aggttagaac gccagatgaa caggttgcgc gggtggtttt
     9901 taccgttttc tggttgttct gccgcaaaac ggatggaacc ctctttcagg gattccaccg
     9961 tatagtccac cggattcatc ccggcttttt ccgcttcgcc agcgatagtc agcgggttag
    10021 tgcctaactg cggtgcagac ggcagccagc ccatgcgttc cgcacggacg ttaaagtcga
    10081 tcaagtgtcc ggtatagcgg gatttgtccg ccatcggtga cagcaactct tccgccgtga
    10141 cggtttcata acgccactgg ctggagtggt tatagaaata agaagtgctg ttcatgtgac
    10201 gcgccggacg ctgccagtca agggcaaacg ccagcggctg ccagccggtt tgcggacgca
    10261 gtttttcctg acctacatag tgcgcccagc cgcccccgct ctgaccgaca cagccgcaga
    10321 aaatcagcat gttgatcaga ccacgatagt tcatatcgag gtgataccag tggttcagcc
    10381 ccgcaccgac gataatcatc gaacgaccgt gcgttttatc agcgttatcg gcaaattcac
    10441 gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat ctgctcggcc caggccgggg
    10501 tataagcttt cacatcgtca tagctggttg cacagttaac gtcgttcagg ccacgttcca
    10561 gaccgtagtt tgccagcgtc agatcataaa cggtggtcac cagggcggtg ctgccatcag
    10621 ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac gttttccagt tccactttgt
    10681 tgaagtgttc cgtgccgtcg ccaccaaagt acgggaaacc tacctcagcg atctcatcct
    10741 ggctacccag caggctcagt tgcagctcgg tttcttcgcc agttttgccg tcgcgctgct
    10801 caagattcca tttgcccttc tcgccccagc ggaagccaat agaaccgttc ggcgcaacca
    10861 tttcgccatt ggtattaaag gcgacagttt tccattccgg attgttttcc tggcccagcg
    10921 catcaaccag atcagcagcg cgcagcatac gacctgcagc gtagtaaccg tcgcgttctt
    10981 ccagcatcac cagcatcggc atgtcggtgt agcgacgcac atagtcggtg aaatactggc
    11041 ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc catcgccagc gccattgccg
    11101 catcggtgcc ctgtttcggt gccagccaca gatcgcacag tttggcgatt tcagcgtagt
    11161 ctggtgtgac ggcaacagtt ttggtccctt tgtaacgcac ttcagtaaag aagtgagcat
    11221 ccggggtacg cgtctgcggc acgtttgacc cccaggcgat gatgtagctg gagttgtacc
    11281 agtcagcaga ttccggtacg tcagtttgct cgccccaggt ttgcggagac gcaggaggca
    11341 agtcgcagta ccagtcgtag aagcttaagc aagtaccgcc aatcagcgag agatagcgtg
    11401 cacccgatgc gtaagaaacc atcgacattg ccggaattgg cgagaagcca gcgacacggt
    11461 ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc gatcagttcg ttcacttcct
    11521 gccaggaaga acgaacaaaa ccaccgcgtc cacgcgcctg cttaaagctt ttcgctttat
    11581 cggcgtcttc aatgatagaa gcccatgcct caaccggatc gctatgcagc gccttcgctt
    11641 cacgccacat tttcatcagg cgtttgcgca tcatcgggta tttcaggcgg ttggcgctgt
    11701 aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg aggttcatgg tttggcagat
    11761 ccggacgggt acgcggatag tcagtctgct gggtttccca ggtgaccaga ccgtttttga
    11821 cgtagatttt ccagctgcag gagccggtgc agtttacccc gtgggtagag cggacgattt
    11881 tgtcatgctg ccaacgctgg cgatatccat cctcccagtc acggttggta ttgagaagct
    11941 ggccatgccc atcggcaaag gtttcaccct tctgtttgaa gtagcgaaac cggtccagga
    12001 atttactcat cggttttctc ctgtgggagc ctgtcggccc tctgataaat cgacattgct
    12061 aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga attgataacg atcaagtgtg
    12121 atggggatga aaaataaagt aaattcctaa gatacccctt taggataatt aacagttaaa
    12181 gcataacatg ctgtaa
//
LOCUS       DAZFVO020000146        11982 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFVO020000146 DAZFVO020000000
VERSION     DAZFVO020000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.147"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(322..1113)
                     /locus_tag="ACHKNB_RS19520"
                     /old_locus_tag="pgaptmp_003904"
     CDS             complement(322..1113)
                     /locus_tag="ACHKNB_RS19520"
                     /old_locus_tag="pgaptmp_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HGU4197325.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
     gene            complement(1132..3102)
                     /locus_tag="ACHKNB_RS19525"
                     /old_locus_tag="pgaptmp_003905"
     CDS             complement(1132..3102)
                     /locus_tag="ACHKNB_RS19525"
                     /old_locus_tag="pgaptmp_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HGU4197326.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGFRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            complement(3277..3492)
                     /locus_tag="ACHKNB_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /pseudo
     CDS             complement(3277..3492)
                     /locus_tag="ACHKNB_RS19530"
                     /old_locus_tag="pgaptmp_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(4213..5232)
                     /locus_tag="ACHKNB_RS19535"
                     /old_locus_tag="pgaptmp_003907"
     CDS             complement(4213..5232)
                     /locus_tag="ACHKNB_RS19535"
                     /old_locus_tag="pgaptmp_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HGU4197327.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PDKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            complement(5374..5565)
                     /locus_tag="ACHKNB_RS19540"
                     /old_locus_tag="pgaptmp_003908"
     CDS             complement(5374..5565)
                     /locus_tag="ACHKNB_RS19540"
                     /old_locus_tag="pgaptmp_003908"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197328.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            complement(5547..5804)
                     /locus_tag="ACHKNB_RS19545"
                     /old_locus_tag="pgaptmp_003909"
     CDS             complement(5547..5804)
                     /locus_tag="ACHKNB_RS19545"
                     /old_locus_tag="pgaptmp_003909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197329.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            complement(5978..6193)
                     /locus_tag="ACHKNB_RS19550"
                     /old_locus_tag="pgaptmp_003910"
     CDS             complement(5978..6193)
                     /locus_tag="ACHKNB_RS19550"
                     /old_locus_tag="pgaptmp_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197330.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            complement(6414..6671)
                     /locus_tag="ACHKNB_RS19555"
                     /old_locus_tag="pgaptmp_003911"
     CDS             complement(6414..6671)
                     /locus_tag="ACHKNB_RS19555"
                     /old_locus_tag="pgaptmp_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197331.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTHKGT"
     gene            complement(6740..8437)
                     /locus_tag="ACHKNB_RS19560"
                     /old_locus_tag="pgaptmp_003912"
     CDS             complement(6740..8437)
                     /locus_tag="ACHKNB_RS19560"
                     /old_locus_tag="pgaptmp_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HGU4197332.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(8533..9669)
                     /locus_tag="ACHKNB_RS19565"
                     /old_locus_tag="pgaptmp_003913"
     CDS             complement(8533..9669)
                     /locus_tag="ACHKNB_RS19565"
                     /old_locus_tag="pgaptmp_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4197333.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            10345..10553
                     /locus_tag="ACHKNB_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /pseudo
     CDS             10345..10553
                     /locus_tag="ACHKNB_RS19570"
                     /old_locus_tag="pgaptmp_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            10540..10729
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /pseudo
     CDS             10540..10729
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS19575"
                     /old_locus_tag="pgaptmp_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(10732..10920)
                     /locus_tag="ACHKNB_RS19580"
                     /old_locus_tag="pgaptmp_003916"
     CDS             complement(10732..10920)
                     /locus_tag="ACHKNB_RS19580"
                     /old_locus_tag="pgaptmp_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HGU4197334.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            11455..11596
                     /locus_tag="ACHKNB_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /pseudo
     CDS             11455..11596
                     /locus_tag="ACHKNB_RS19585"
                     /old_locus_tag="pgaptmp_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         3072 a         2748 c         2512 g         3650 t
ORIGIN      
        1 ccatttagca taaaaatatt taattttata tgaccaacag ttcttgttaa agaatattag
       61 ctaattttca ttatattata aacaaagagt tctttgctgt gatggatttc acattttatt
      121 gtcttgttat gtattccttc tggtttgaac agggtgcttc agggattttt attactatta
      181 cgcttttttg gaggtgatct ttacataagt atatctggaa atggatatga aaaatcatta
      241 tgtggttttt tacacggatg tcggtaggct attctcgtat tgataatacc ctgcccataa
      301 atcagggcag gaatagtatt attaatttcg ggagaagctt aaggttgcca catcgacagt
      361 ttcatcacac agactcttgt ccggaaatac tttcttattt ttgactttag ccagccattg
      421 cccgacatga agggaaggta cagtgatgat accgtcctca ttacttacaa aggtaagttc
      481 tgctttatga gccgaatgtt tatgctcagc tgcatgggta tgacgggatg atttcagata
      541 actgacagag tcaagctcca gagtagctcc ggcaagcggg gatgttttat acagaacttt
      601 cagcttcaca tcttcaccga aacgactgat atcggataat ggaatgattt caagctcatc
      661 ccctgttgga gactgataga gttgtgctgg catctgctgt tcaccaggaa taaaagattt
      721 tgcatattta gtcacaaact cacagtatgc cacatcattt ctgccctccc gactgacagg
      781 cttccatcct gacgaggttc tcgaccagta tcccggtttc attttcgttg acagaacgta
      841 gctattaccg ttggctgcag actcagtttt atagacataa tccgtggacg ttggcttcag
      901 cgttattatc tctttcccgt tataaagccg gggattttca aagaaattcc ttctgtcagg
      961 aattgtgccc tttgagggga aattatgacc ataaccaatc tctgcggtaa cgttgttctc
     1021 tgcctgttta ccggtaatcc agacatcgtg ggccagggca tgctccgatg ctgtcagaca
     1081 aagagctaag atatagcctg tatttttcat catgatttat ttcctgcgag gttaccactg
     1141 ataacgggta taaagactga cagttctgcc tgaaagcggc acacctttgc cattcatggc
     1201 aagcttgttg aaggcattat ccagagtcac tgatgcctca aagcttttaa cacttacagg
     1261 cttataagac agtgaaaagg aatgtacggc atagccagcc tgatggggat cgcctttttt
     1321 cacatgcgtg gctgctgccg aaagttcagc acgccatccg gcagaaactc ctccatgacc
     1381 aacaggtata ctgatgtcac tgatgagtgt gtcaggattg ccggagccca gccattccct
     1441 gctactggca tccatacttt ccgtgcggtt ataagaaagc atccagtcaa aataatcaga
     1501 ctggtaatta ccctgcatat cccatcccca gatacgggcc cgggatacat tataagaata
     1561 agaacgcatc cgtttcatat ccacgcgcgt ggcaatatag tttctggcat ccgttccgaa
     1621 atatccgcct ttcaactgca gccggtcatt gtctgtaagc aggccgtcca gttgaatacc
     1681 tgcgccaatc tcacgggtaa tgttattttc tggtttcaga ttaaggttag gtacccagta
     1741 attgggttta cccttgcggt aaaaatgtac atcatccctg tacatttctg ccattgtggg
     1801 ggcacggaat gcagaggata tggagccgta catcatcagc cagtctgttg gtgagactga
     1861 aatcgccgcc ctgggggaca ggcgttcggc tttcagtttt ccggctcggg gattgaagct
     1921 cttgtaccgg tcataacgga aaccgacgat aatgttaacc gggttgcttt tcattgtcat
     1981 ttcatcctgg aaatacaacg atgtaaagtc aatgtttcct tccggatata gtgtggcaga
     2041 gccttccggc ttctgctgct gacggtaata ttcggcgcca taacttaact gatgggctcc
     2101 cggaaaaatc agagtatggc tcctgttgac aacattaaca ccggaggttc tgttgtttcg
     2161 ccattctgct gttttgttgt gaccgtttgt cttgatagtg atatcactga gatataacgt
     2221 tgactttaca ttgatgaggc tgttatccgc aggggagtac tggtaccaga actgcacgtc
     2281 attttgtgct gtttttctgt cacgcaggcc gttgtcacca tgtgtcagag tgggattgcc
     2341 cggttcagtg gtgttattct ggtagagacg caagctggca cctgctttgt tactgtctgt
     2401 tatactgaca gagcctttcg caaacagggc tgcaggtttt tccttgttag gtgcacgctc
     2461 accatcactc tgatagatat ttcctctttt acgcataatg acagaaagaa gcgcatctgt
     2521 ttttccggtt ttaccgaacc aggtgagccc cgagcctgtg ctgtggtcac cactggcgat
     2581 atttccccac agacttacac catttgtctc tccgggggga agaaaatctg cagcatcggc
     2641 agttctgaag tccaccaccc cccccagccc gccactgccg tacagactgg agtttggtcc
     2701 gcggacaact tcgatgcgtt tgatgagtgc cggatccaga taagtgccac tgctttttgc
     2761 catgtcactg agttgacgga cgccgtcaac aagaacaagt accccgcttt tgtcatagcc
     2821 gcgtagatta aaggtctgcc cattggtccg tcctgcacca gtctggctga gacccgcaac
     2881 acctttcagc ataccggctg atgtgaccgc attctgactc catggcgtgt cattcgtgat
     2941 gacactgacc atcgacggcg tttcaaatac cgtgttctga tttcctgtcg cgacaacggt
     3001 gattgtatct ttagccatag aagaatgact tgtcgctgcc tgtaacccca ccggaagtat
     3061 gattaatgta caaatgacag ttctgattac gttcatatac attcgaaaat atatgtcaat
     3121 acagcaatgt tataacataa cattttgttt gtgcaaccac tattacgcag tgttcagtgc
     3181 taatctgtta atagctattc taatacccag tcgaaccact aagagtgtat atggtaagcc
     3241 tgcccttgag tcactatttc ctgacaacta ctgcatctaa ggtgacgtta ttcgctgacg
     3301 ttgatattcc cttggtggga gataacctaa cgcactatgc ggatggtttt cattgtaata
     3361 cccgatctcc actgcaagat tatgcaatgc cgttcttaca ttagatttcg gcatgcacgc
     3421 gatgtagtct ttcttcatcg ttttcacgaa ttgttcgtaa gcgatgagat ggtcttcccc
     3481 cgtattgcaa gagtacagca actatgatag ggacaggttt actttcgtat atccggtttg
     3541 ttcatgaagt tattgcttct ggccttgatg agacattcgc gcgctccgac cttatcgctt
     3601 actcggcctt actggcctgg ctcatcttgc aggttttcgt aacgtcggtc tgacctgttt
     3661 ttcatcattc tgcattcttt gcaatttcag ggtaagcttt actcctactg attttacgta
     3721 gattattgat tttgttttgc tgcgggcaaa tacatctcat tgcgggcgat aattgcccag
     3781 gcgatccgaa ggagtttatt tgcaagagca acaactgcct ggttgaagcc acgcttttcc
     3841 cgaatacaat taacccacac acgaagtgaa tcagttttct ctgaggctct gaacaatacc
     3901 gctctggcac cctgtatcag catagatctg acatattgat tatatgaaca catcccgttg
     3961 caaggcatcc attgccttga cagtgacggg taggttgcag ctttatatcc ggcttttttg
     4021 cagccatgaa gctgcgagcc cctatgtaat tcgctgactc acacctcatt tctccagcga
     4081 gcttttagct caacacaata ttcaggttta gtgagtcccg gtcttacctg tcgttgtcat
     4141 cactatggcc gccagagtta cctttcagta ttcatccttt cattaaaagt taatatttat
     4201 cggtatcagc gattaatatg atctgatact gacatgattc ttgtcgtatt tacttccatg
     4261 agctgctatc gcccatactg ttcgtgctag tttgtttgcc atggcgacga ttgccacgct
     4321 ggttggacga cgttttttca gttcggttat ccacggacca ggttctttgg gcagtaatgc
     4381 tgccgccctt gcgctatgaa taaacaaggc tcgcagatat gtattaccac gtttgcttat
     4441 ctccagcagg cggattttcc ctcctgagcc agtctgtttg ggcaccagac caacataagc
     4501 cgcgaactct cgccctgatt taaacgctga tgcttccctc atggtggcca ccgctgccgt
     4561 tgcaataagt ggcccaacgc cgggaatatc cagcaaccgc ttacaggttt cattctgtct
     4621 cgccacgctg gtaagctttt tttcaatatc ctctatcagc gagtccagct cattcagccg
     4681 gttgtactgg ttttccagca cagtaatgag atacggcggc agtctctctt tcatccgctc
     4741 cagtgcttca gggagctctc gctccatcgc tgcccgacct ttgtgaatgg ttttaccaaa
     4801 ttctaggagc gtgccgtgca gagaattaat ctgcatggtg cggaacttaa ccaattgtct
     4861 gcgggaacgg tgcaaaacca gcactgactg ttgttcttct gttttaaccg cgatgtcctt
     4921 atctggctgc tgaacagcca tccagatagc ccgggcatcc atcacatcat tcttatttcc
     4981 catgacgaat gccttaacaa agtgggcctg taacaaccgg actttatgcc ccagctttgt
     5041 cagttcacgc ccccagtgct gagagcctct acaggcctcc ataccaatca ggcagggttc
     5101 ccgattacag aagaacatca gaaaatcctg acgacgtagt tgcttatcaa tcacttcacc
     5161 tgtgtgctcg tcgatgaaat gaacctgaat cagatgtttt gcgatatcaa cgccaaccgg
     5221 tgtatatttc atttgtggag tctccagtct gagggagctt tatgcatcct gtattgggca
     5281 ctatgatgcc ggaaatctgc gaggctccac accttccagt atttcccttc atccatcaca
     5341 gagcacctga cttaatgggg tgggatgtgt tcattacatt tctccaaatt ttgttatacc
     5401 cagaagaacc tgcttcccac ctgtgctgaa ctgtcgggga accagtccca gagtcgcaga
     5461 tgcatctctt cctcgtctaa actgttttcc atcccccatc cagcttttta ttgcctgact
     5521 aaccagaggg ctgaatctag gaatggctaa caagcgtttg cacataggat cttggcggac
     5581 atatttttca gttcagaatc ataccactcc agttcattat ctagtgtaat caaccgtata
     5641 tactgccgat gcaataacgt cttcatggta tcagtgagtt cgttttccgc atcctcaagt
     5701 aatgccggta atttttggcg taattctgtt gcgcctttac tgaacacaat gccatattca
     5761 gcaagaagtc cacgaacgtg atttatcagt tgtgtccttt ccatcgaaac caaccggcgc
     5821 attttcaaaa aagtctggac gtcctgttac tccagagttt ttattggtac ggggcgtata
     5881 gttccatgtt ggcaggcctc agctattgcc tgagcgtcgt cataatcatt tttctggcgt
     5941 cgctgataag ctttaacatg ttgcgccgga agaaatatta catcatggtc gagtttcctg
     6001 atttccctgg cccagtgatg ggatgcccgc gagcctccat cgcaacagtc attttaggct
     6061 gctgcaccag ccagttaagc agttgattgc gagaaagttt gatccttcct gcgggtttgc
     6121 catgagcatt gatactgaaa agataaaagt tcgttttagc cagatcgatg ccggtgattt
     6181 tctgttgaga catggtatcc ctcttacttt tgtggttaaa gtttatccat catgacgtaa
     6241 ttaaaggttg tcgtgaagga gggggaagac catctcatcg cttactgatg ttcctgttac
     6301 tatatctatt gaatcctggc tataagttcg ctgtttaaag aattatatga atagcaccaa
     6361 ttaataagtt aatattaaaa agaagacaga ccttctaaat aaacgcattg aatctatgtt
     6421 cccttgtgag tgtttttacg tttggcggga ggcctggaac ctcgggattt aacttttctt
     6481 tttatatttt tgataaaatc atcaatagaa aactgaatcg tctctgataa atactgttga
     6541 gctatatctg tactctgttt ttctaaatca gaagctttct ttgtttcaag aacagatttg
     6601 attaactcct tacgttgtgt acaccaaatc tcgtcagatg aacagtattt tttattttcg
     6661 tgtttgttca ttatttattg ctctaactga tttatttccc tatatccagg gagtagatat
     6721 atctactccc tgcttattat catttcacgt tagttaaaga tttttttata ttctcaaatt
     6781 tggcttggtt gatatcactg atcggttttt gacttgacgt gatgaagtca atcataacag
     6841 ccttcatatt cttaactaca tcaggatggg atgcagagag atctttagtt tgttgcagat
     6901 cgttgatatc gtacagagct agtttattct ctgcataggt gtaaaccaga gaataatcgt
     6961 tgcttcttac agtgtatgca aactggctta agtcttctgt atgtgggttg tgcggataat
     7021 catcagactc ttttctcacg aatttatgat atccgttcca gaatgcagtg ttctcttcgt
     7081 cccaccagtg agaataagag gtaatccagg acagatattt gtgaggttcg ccagctgcag
     7141 atttcttatc agctcctttc tgtacatatg gcaataatga tacaccatct agtttcagag
     7201 aagcagggat cttgatatct gcggcatcta aagcggtcgg atagaagtcc attgatgaaa
     7261 ccagtttgtc ataatcaccc gttttcagct tgccattcca ccacatgaac ataggtgtat
     7321 gcataccacc cggatacggt tggcctttgt aaccagcctg cgcaccattc agtggcagtg
     7381 gaccatcaat tacagcaccg ttatcagagg tgaacagaat gatggtgttg tcgtactgac
     7441 ctttttcctt aagtgtattc aggatctttt taaccccctg gtcaacggag taaattgacg
     7501 cataatagtt atcagcagtc ttgctaccag tgttgaattt ctcctgatag tttttaggcg
     7561 caggctgatc gttaggcagg tgtggtgcac tgtatgccag ataaatcata aatggctgtt
     7621 ccagtttgct tgcacggtca attacaccaa ttgcttcatc agttaattga tcactaatgt
     7681 acccttcagc tttaatgttt tcacggttac ggaacaatga cggggagtta taaagtgaaa
     7741 cgccggatgc gtgataaccc atgaagtagt tgaagccacg attctgaggt tgccattctt
     7801 ctgcggagaa ggtggtgaag ttatcgtggt agtcacgggt ttgtttttct tcaggcacac
     7861 ctacgttgct gatttttgat aagtgccact taccgattgc tgcagtgtaa taaccatgat
     7921 tctgaagtaa ttcagacagg aatgtttctt ccagagggat accatcctgt gcatcagaat
     7981 ttgaatatac accgaagcga gcaggagctc gcgccgtcag gattgcggca cgtgaagggc
     8041 cagagacacc gtgtgcaaca tagccgttag tgaattttac accttcgtcc atcagggccg
     8101 ctaatgttgg agtggatttt tttgcagctt caattgcttt atcaatacct attttgtatg
     8161 tatcaacaac atttcggtta tccatcgttt tagggtcaaa tgaactatca tcaaaaggca
     8221 attgtccata ccctaaatca tccattgtta aaacaatgat atttggctta cctttagtgc
     8281 tgtattccgt cggcgtgaag tccgcaaagg caacattggt ttttgttgct ttgagcttaa
     8341 catcatctgc tgcatttgca gtactcatca tcagaccaga tgctaatata agtgatattg
     8401 atgagttaag cagtttttta tttaactgat gttccatatg gtattccttc tgagttgcat
     8461 cacgaacatt tattaataat aaaagcctct gatataaata aaaactatgt aaatcgttta
     8521 aatggatacc tgttaaaact gatacatcat accaacagca agtatattat cggttgcaac
     8581 tccagccttt ttggtaaatt cgctttcatc aataaggtta attttgtaat caataagaag
     8641 tgacatgttt ttgttcagaa agtagtttgc gccaacatca atatactcaa caagagactg
     8701 atcgccatag tttactattc cacctttaac atccatccct ttggactgaa cgtaagccag
     8761 tgaaggacgc aggccgaaat caaacatata ctgagccaca acttcaatat tccgtgcttt
     8821 atctgcgatg cctgcgctac caaatggagt catatttcgg gtttcagagt agtcagctgc
     8881 cagatacagg tcattatcat tatacttgac cccaacacct gcaaactccg catgttttcc
     8941 cccggcaatc cagcgaggcg taataacttc ttttccttct gaatctttcg atgtcatgaa
     9001 acgtttacgg ccttcttttt cctgcagact ggttctgtcg gcattagtgt aagcggcaac
     9061 gattccaaaa ccttcatagt catagcttac ggagaaacca tatccatcac cattagcttc
     9121 aagtaaatta tcacggtcat ttttaccctg atattgagca gaaaaagtaa gtccatcaac
     9181 aagcccgaag aaatctgtat tgcgccaggt cagaactccg gttgttctgc cggtcatata
     9241 gttatcagag taagtccagc tgtcaccacc aaagtccgga agtacgtcag tcagagcccc
     9301 cacatcatag gcaacaccat agtttcgccc ataatcaagt gaaccgagta tattgtggcg
     9361 aatacctgca aatgccagac gatttttatc gttgcgtact tcactttctg tcttaaaccc
     9421 caggaactgg tattcccact gaccaaagcc agtcaggctc tcattaattt gtgtttcgcc
     9481 tttgaaacca agacgtgcaa atgttctgtc gccatccata gaaccatcag agatgtaatg
     9541 acgggcttta acgcgaccat aaaaatccag tttattaccg tctttattat agatttctgc
     9601 tgcctgtgtt gcctcacaca gcattaatgc agaaacgaca gagcacacaa ccatacttaa
     9661 ttttcgcata aaaaaatccc ctatgcacat ttttagtatg gttttaaaaa ttaaactatg
     9721 gcgaaaaaat tatcactatg caaagatgta acaaaatatt gctcagtgat ctatattgtt
     9781 gtttttattt gaggttttat attttctagt gtatttgact tttctttctc aagtgttatt
     9841 tcttgacctt tatcacagaa ccatctccct ggatatcccc atcacattgc atgaaaagca
     9901 agttttacca atcttgattt tcagtgttaa ataagattaa taactgtaat tctgcaaaaa
     9961 aaagaacacc atatgtagta tgactacgta ttccactctg ttcgttcacg gactggcatc
    10021 gaagtctgag gtgtactggc aatagcggat acagtcattt gttctttttt taagcattca
    10081 tccgatgata cttttcctga atgctaccgg agtggtattc ccctgccgtg agggagggcg
    10141 ctgacggtta taaaaatcct gatatatacc cattcccgta tgacagcgat ggcgtcacat
    10201 ctctgttcta ccatcttcac agaaatattt cgttcattag cattccggta ttcgtataac
    10261 caccttcggt ggagaaagaa taaacaatcc ctggaatgcg atgagcgctt gaccagaaaa
    10321 gcatcatatt gcttcatggc gcatcagcga ccacaggatg ccgcaaaagc cgaaaaacgg
    10381 accagagcgc ctgcaatcat tcctcgcctc agtatctgtt gcccatggag tggccgataa
    10441 cagatcattg tcggatgagc cttatcccct tccttcccgg tggtatccgg atgtgctgcc
    10501 agccttctca gttaaccgat gttttccggg ctttttgcca tactgaggac tgataacgta
    10561 ttcctggtcg taaaacccgt caatatgcgc cggggcatcg acaaaactga cacagtacgt
    10621 ccggaaagaa cttaacgctg catggcacga caatgctgcc tttgtcttta ccaacaaggc
    10681 tcgttcccgc ataaaagttc tacgccggga taaacatggc gtatggctgc gtcaggcctt
    10741 cactacttca caccggaagt cttccaccct gacacgaata taccgtccgg gctctttcgc
    10801 aggtgcaaac agtttttcac attcggcgaa tgtcatttct gaatcctggt aacgcaacca
    10861 ctgaaccggc atattaccgg gtttgattat tgtcgcagtt attttatatc gtttccccat
    10921 atcgcctctc atcctgacaa caaaattacc ggctgagcca tcactccggt aaagcatgcg
    10981 aaaaaaagtg agcgtctcag caatggctca tgcgatgatt ctgtacgggc accgatggca
    11041 cctcagaata caacaaagat atcagagtaa catgtctctc tcgttatttt tcagagttct
    11101 tgacaccctc cttctgccac attgtcaacg ccagtggaga cttctgctgt cagttttttc
    11161 atccatacag agtgacatta attatcctga acaaaacgaa atatcctgaa tatatttatt
    11221 ttctcctggt gccggagaca tgatgatgta caggctcagg tgcggtgtga atgtaaaatg
    11281 gtatgttaat tattcatttc tgaatgcaaa ctgaatgcaa acttattccg aattgaattc
    11341 tcctgacacc acatgataaa aatgaggata taataaaata cttctcttct ggatgtgcca
    11401 ttacctgccg ttgctgtaat acggcgggta ttttttactg acatatcggg aagccagcag
    11461 acgttcgtag ctacatcccc gaaagtggca cactgcaaac tataatacga gtaaaacatc
    11521 gatcaccgta gctgatgtgt tcaacgatcg tgcactagcg ttctgcgatt ctcagggact
    11581 gcctgttctg agaatcggcg agtccgcgat cggatacgat gcgatttcgc agtgactcag
    11641 ggattgcatc tgaaaaccct ccggctatgt cggtaaatat ttatttctat gaaccgtacg
    11701 attcagatag cattcgcccg gccccgtaag aataatctcc ggacaaataa gatttttcct
    11761 gaaaaagcgt ctagtctttt gatatcgttg tttttatgct aatcagggag tatgtgtgat
    11821 gattgtctgt tacgttattc ttgttaaccg tccgtttgaa acagagattg ccgggaaaaa
    11881 gatatttttt cagaaaaatg acgtcctgtt gacagataac atcagccagt cctttttcct
    11941 gcgccatccc ggtaatgtta ctgagctccg gattgatgaa tg
//
LOCUS       DAZFVO020000147        11976 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFVO020000147 DAZFVO020000000
VERSION     DAZFVO020000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.148"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2104
                     /locus_tag="ACHKNB_RS19590"
                     /old_locus_tag="pgaptmp_003918"
     CDS             <1..2104
                     /locus_tag="ACHKNB_RS19590"
                     /old_locus_tag="pgaptmp_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HGU4197335.1"
                     /translation="YPQGLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKA
                     AQRSITAPDRIGEGKSWVYQRDTMIERGDNSGVHQYGPAEHFTHIISDKPSPKDEYIA
                     YAINIPDYELAADVYNINVTSPSEQQETFKILINPEHLRQTLERKSLTAVQKSQCEII
                     TPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYW
                     FHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYP
                     SADIRAKISKDIEGGIPSYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDN
                     ISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSK
                     HEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYER
                     DDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNL
                     FNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLN
                     KGCSTYQQLYNEFHALYIDKAGIWSAFEGIFATKNVLAFSREDSVNINHSPHDGLVII
                     NKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTQKIIPPGKVFTLRGGSLNINIPG
                     RSALLLGKTGEPPNYLYL"
     gene            2440..3417
                     /gene="glaH"
                     /locus_tag="ACHKNB_RS19595"
                     /old_locus_tag="pgaptmp_003919"
     CDS             2440..3417
                     /gene="glaH"
                     /locus_tag="ACHKNB_RS19595"
                     /old_locus_tag="pgaptmp_003919"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HGU4197336.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3436..4704
                     /gene="lhgO"
                     /locus_tag="ACHKNB_RS19600"
                     /old_locus_tag="pgaptmp_003920"
     CDS             3436..4704
                     /gene="lhgO"
                     /locus_tag="ACHKNB_RS19600"
                     /old_locus_tag="pgaptmp_003920"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HGU4197337.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4727..6175
                     /gene="gabD"
                     /locus_tag="ACHKNB_RS19605"
                     /old_locus_tag="pgaptmp_003921"
     CDS             4727..6175
                     /gene="gabD"
                     /locus_tag="ACHKNB_RS19605"
                     /old_locus_tag="pgaptmp_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HGU4197338.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6189..7469
                     /gene="gabT"
                     /locus_tag="ACHKNB_RS19610"
                     /old_locus_tag="pgaptmp_003922"
     CDS             6189..7469
                     /gene="gabT"
                     /locus_tag="ACHKNB_RS19610"
                     /old_locus_tag="pgaptmp_003922"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HGU4197339.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7707..9107
                     /gene="gabP"
                     /locus_tag="ACHKNB_RS19615"
                     /old_locus_tag="pgaptmp_003923"
     CDS             7707..9107
                     /gene="gabP"
                     /locus_tag="ACHKNB_RS19615"
                     /old_locus_tag="pgaptmp_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HGU4197340.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9128..9790
                     /gene="csiR"
                     /locus_tag="ACHKNB_RS19620"
                     /old_locus_tag="pgaptmp_003924"
     CDS             9128..9790
                     /gene="csiR"
                     /locus_tag="ACHKNB_RS19620"
                     /old_locus_tag="pgaptmp_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HGU4197341.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9791..10240)
                     /gene="kbp"
                     /locus_tag="ACHKNB_RS19625"
                     /old_locus_tag="pgaptmp_003925"
     CDS             complement(9791..10240)
                     /gene="kbp"
                     /locus_tag="ACHKNB_RS19625"
                     /old_locus_tag="pgaptmp_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HGU4197342.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10324..10482)
                     /locus_tag="ACHKNB_RS19630"
                     /old_locus_tag="pgaptmp_003926"
     CDS             complement(10324..10482)
                     /locus_tag="ACHKNB_RS19630"
                     /old_locus_tag="pgaptmp_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4197343.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10665..10964
                     /locus_tag="ACHKNB_RS19635"
                     /old_locus_tag="pgaptmp_003927"
     CDS             10665..10964
                     /locus_tag="ACHKNB_RS19635"
                     /old_locus_tag="pgaptmp_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HGU4197344.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10974..11498
                     /gene="ygaP"
                     /locus_tag="ACHKNB_RS19640"
                     /old_locus_tag="pgaptmp_003928"
     CDS             10974..11498
                     /gene="ygaP"
                     /locus_tag="ACHKNB_RS19640"
                     /old_locus_tag="pgaptmp_003928"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HGU4197345.1"
                     /translation="MALTTISPHNAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(11545..11949)
                     /gene="stpA"
                     /locus_tag="ACHKNB_RS19645"
                     /old_locus_tag="pgaptmp_003929"
     CDS             complement(11545..11949)
                     /gene="stpA"
                     /locus_tag="ACHKNB_RS19645"
                     /old_locus_tag="pgaptmp_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HGU4197346.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2994 a         2952 c         3089 g         2941 t
ORIGIN      
        1 ttaccctcaa ggattaaaag acagagaaat tatatcaact tttcacgcag aaaaagttat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttatcgaga
      121 gttgaataaa gcagcacagc gtagcattac agcgccagac aggataggag aaggaaaatc
      181 atgggtttat caacgagata caatgattga aagaggtgat aacagcggtg ttcatcagta
      241 tggtcctgct gaacatttca cccacattat atctgacaaa ccgtccccaa aagatgagta
      301 tattgcatat gctattaaca ttcctgacta tgagctggca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccgaac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 aacccccaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttg ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaagggtt actggtttca
      661 tgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt
      721 tattgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaattgggg gtagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaagg tggtatccct agctattggc aaggtcaggt
      901 tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacacccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cggcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg gttacacaat actaacttat ttaacccaaa
     1261 atatcataaa aaagccgtta tgaattacat ggaatatctg gtaacttgtc agttggatga
     1321 acaacaaatg tcatcactgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaaa acgataaaag ccttttcatt tggtggagat cttcaaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gtatcccatc agaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggta atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tggccgctct gccgttcagc aggcctttag tttactggga
     1621 tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcca cgcgtgttgg
     1681 cggtgaggcg tggcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtatat ttgcaactaa
     1801 aaacgttctg gcctttagtc gtgaggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata tataaaaaca tggggagtaa tagcgttaac attattatta ataataccca
     1981 aaaaattatc ccccctggta aagtatttac gcttagaggc ggatctctaa atatcaatat
     2041 tcctgggcgt agcgctcttc ttttagggaa aacaggagaa ccgccgaact atctctattt
     2101 ataatttaat gttatatctg ccccgataaa acggggcaga taatatgttt agtttactaa
     2161 cggtcatttt gcagtgaagc catttactgt tttttatcga ccagataatc tgttctctaa
     2221 tgttaactcc ccctaacctg ttgctttagt tattcatttc ctgtctcact ttgccttaat
     2281 accctacgtt aaatgttact aatttgttgc ttttgatcac aataagaaaa caatatgtcg
     2341 cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat
     2401 cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact gaccgccgta
     2461 cacaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat cccgtcggcg
     2521 caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa
     2581 caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta
     2641 ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg
     2701 ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtgg gtatcgatga tgtggcgcag
     2761 gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc
     2821 gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac
     2881 agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa
     2941 gagatcaccg attacgtgct gatgatgaaa atcgatgagc aaaacatgca gggtggaaat
     3001 tcgttgctgc tgcatctcga tgactgggaa catctggacc actatttccg ccatccactg
     3061 gcgcgtcgcc caatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc
     3121 catccagtgt tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc
     3181 gtccagccga aagacttcga agaaggcgtg tggttgagcg agctttcgga cgccattgaa
     3241 accagcaaag gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac
     3301 ctgttctggc tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg
     3361 atgcgtcagc gcggctactt cgcttacgcc actcaccact accagacgca tcagtagcgc
     3421 aaaggaattg agcggatgta tgattttgtg attattggcg gtggcatcat cggcatgtcg
     3481 accgccatgc aactgattga tgtctacccg gacgcccgca ttgcgttgct ggaaaaagag
     3541 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtttat
     3601 tacacgcccg gtagcctgaa ggcacagttt tgcctggcgg gaaaccgcgc cactaaagcc
     3661 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacctct
     3721 gaactcgaaa tggaacggat gcgcgcgttg tgggaacgca cggcggcaaa cggtatcgag
     3781 cgcgagtggt taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggc
     3841 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtcacagc ggcgatggca
     3901 aaaattttcc aggccagagg cggtgagatt atttataacg ccgaagtcag cgccctcagt
     3961 gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg gcgaatatga agcatcgacg
     4021 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     4081 ggttttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     4141 attgttaacc atctgattta ccccattccc gacccggcaa tgccgttttt gggcgttcat
     4201 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     4261 cgcgaaggct atcgcaagcg cgacttctca tttagcgaca cgctggagat tttgggctcg
     4321 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     4381 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     4441 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cggtatcgcc ggacggcaag
     4501 ctgattgacg attttctgtt tgtcaccacc ccgcgcacga tccacacctg caatgcgccc
     4561 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     4621 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     4681 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     4741 cagtaaatta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     4801 cgaggtcatc gatgtcacca atccggcgaa cggcgacaag ctgggtagcg tacccaaaat
     4861 gggcgctgat gaaacccgcg ccgctatcga cgccgccaac cgcgctctgc ccgcctggcg
     4921 tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac tggttcaatt tgttgatgga
     4981 gcatcaggac gatttagcgc gcctgatgac cctagaacag ggcaaaccgc tggctgaagc
     5041 gaaaggcgaa attagctacg ccgcctcctt tattgagtgg tttgctgaag aaggcaaacg
     5101 catttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     5161 gccgattggc gtcaccgcag ctatcacgcc gtggaacttc ccggcggcga tgattacccg
     5221 caaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcctg ccagtcagac
     5281 gccgttctct gcgctggcgc tggcggagct ggcgattcgc gcgggcattc cggctggggt
     5341 atttaacgtg gtcaccggtt cggcgggcgc ggtgggtaac gaactgacca gcaacccgct
     5401 ggtgcgcaag ctgtcgttta ccggttcgac cgaaattggc cgccagttaa tggaacagtg
     5461 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     5521 tgacgatgcc gacctcgaca aagccgtgga aggtgcgctg tcctcgaaat tccgcaacgc
     5581 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     5641 tgccgaaaaa ttgcagcagg cggtgagcaa attgcacatc ggcgacgggc tggagaaagg
     5701 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     5761 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcggatg aacgcggcgg
     5821 caacttcttc cagccgacca ttctggtgga cgttccagcc aacgccaaag tgtcgaaaga
     5881 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     5941 gcaagccaat gacaccgagt ttggccttgc cgcctatttc tacgcccgtg atttaagccg
     6001 cgtcttccgc gtgggcgaag cgctggagta cggcatcgtc ggcatcaata ccggcatcat
     6061 ttccaatgaa gtggccccgt tcggcggcat taaagcctcg ggtctgggtc gtgaaggttc
     6121 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     6181 gaacgcgaat gagcagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     6241 gtgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     6301 ttgaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcact
     6361 tgcatccgaa agtggtggct gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     6421 tccaggtgct ggcctacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     6481 caggcaattt tgccaagaaa acgctgctgg tcaccacagg ttctgaagcg gtggaaaacg
     6541 cggtgaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgcgt
     6601 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     6661 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     6721 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     6781 aagatatcgc cgccatcgtg attgagccgg ttcagggcga aggcggtttc tacgcctcgt
     6841 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     6901 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     6961 gcgttgcgcc ggatcttacc acctttgcga aatcgatcgc gggcggcttc ccgctggcgg
     7021 gcgtcaccgg gcgcgcggaa gtaatggatg ccgtcgctcc aggcggtctg ggcggcacct
     7081 atgcgggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg tttgagcagg
     7141 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagtt gaaagacgga ttgctggcaa
     7201 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggggcgatg atcgccattg
     7261 agctgtttga agacggcgat cacaacaagc cggacgccaa actcaccgcc gagatcgtgg
     7321 ctcgcgcccg cgataaaggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     7381 gcatccttgt accgctcacc attgaagacg ctcagatccg tcagggtctg gagatcatca
     7441 gccagtgttt tgatgaggcg aagcagtagc gccgctccta tgccggaggc gacgctgcgc
     7501 gtcttgtccg gcctacgggg atccaggtcg gataaggcgt ttacgccgca tccgacaatc
     7561 tgtacgtgaa caggaagaaa tctatgttgg ccgggtaagg cggagccgct ctccggcaaa
     7621 aagaatcaat aacaattata cgcgtgaccc ggcgcgggaa atgtcggggc gctctcccaa
     7681 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg
     7741 gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     7801 tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc
     7861 tgttcgccgg gctactggtg gttatgatta tgcggatgtt ggcggaaatg gcggttgcca
     7921 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggtcgc tgggcgggct
     7981 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccgctg gaagccaaca
     8041 tcgccgccat gatcctgcac tcatgggttc caggcattcc catctggtta ttttccctcg
     8101 tcattaccct cgccttaact ggcagtaact tattaagcgt taaaaactac ggcgaatttg
     8161 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     8221 tcgcaattag cggtttttac ccgtatgccg aagtgagcgg gatctcaaga ttgtgggata
     8281 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     8341 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac acgccggaaa
     8401 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttatgtt
     8461 ctatttttgt cgtcgtggca ttaattccgt ggaatatgcc cggactaaaa gccgtcggtt
     8521 cttatcgctc ggtactggaa ttgctcaata ttccccatgc gaaattaatc atggactgcg
     8581 tgatattact ttccgtaacc agttgcctga actcggcgct gtataccgcg tcaaggatgc
     8641 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     8701 aaaccccgta cgtggcggtg ttactctcca ccggcgcggc attcttaacg gtggtggtga
     8761 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     8821 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     8881 aaggaagcga aattcgcttg cgaatgtggc tttatccgtg gctcacctgg ctagtcatcg
     8941 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
     9001 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
     9061 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgatca tgatcaggag
     9121 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
     9181 cggtaatttt caaccggatg aaaaattacg aatgagtttg ctgacatcgc gttatgcact
     9241 tggcgttggg ccgttacggg aagctctttc gcaactggtg gcggaacggc tggtcacggt
     9301 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
     9361 cgacgcccgc gccaatatgg aagcgatgtt agtgagtctg gcgattgccc gcggtggcga
     9421 tgagtgggag gcagacgttc tcgcaaaagc gcatctgctg agtaagcttg aggcctgtga
     9481 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacggcaat
     9541 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
     9601 ggcgcgctat cgatttatct ggctgcggcg aacggtgctt tcggtagaaa tgctggagga
     9661 taaacacgat cagcaccaga ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc
     9721 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
     9781 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
     9841 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
     9901 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
     9961 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccaa
    10021 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    10081 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    10141 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga
    10201 gtttttctcc ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg
    10261 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    10321 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    10381 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag
    10441 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    10501 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    10561 gccagtttca gcttacgctt ttactctcat ttacgctgct gaggctggta tatgttgcat
    10621 ttttagttta gagtttactt aatttagaaa atacttaaat aaatatgact gaactcgcgc
    10681 aattacaggc cagtgccgaa caggcagcgg ccttattgaa agcaatgagc catcctaaac
    10741 gattgctgat tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc
    10801 gcattaccgg actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag
    10861 ggcttatcga cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg
    10921 taaatgccat tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt
    10981 tgacaaccat ttcgccgcat aatgcacaag aattaatcgc acgcggcgca aagttaatcg
    11041 atattcgtga cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat
    11101 tatccgtgct ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc
    11161 actgccaggc aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc
    11221 ccgcagaaat atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag
    11281 tagcggtaaa taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg
    11341 gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa
    11401 gtggctttgt tggtgccggg ttactgtttg caggaatcag cggtttttgt ggaatggcaa
    11461 ggttgttaga taagatgccg tggaaccaac gaacttgaga agcgacgccg gacgcgccct
    11521 agcagcgaca tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc
    11581 cagcgcctga gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc
    11641 gttaacatcg gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcgcgtgg
    11701 tgcagcagca gagctattac ctaataactc ttccgggtta attccgtcag ccttcatcag
    11761 ctccagccag gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc
    11821 ttcttcacgt ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc
    11881 aatggagaat tcgcgagcca tcgcacggag agtgcgaatg ttatttaaac tttgtaacat
    11941 tacggacata ataaagaaaa ccttttaacg ccaaaa
//
LOCUS       DAZFVO020000148        11500 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFVO020000148 DAZFVO020000000
VERSION     DAZFVO020000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.149"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKNB_RS19650"
                     /old_locus_tag="pgaptmp_003930"
     CDS             complement(171..2204)
                     /gene="betT"
                     /locus_tag="ACHKNB_RS19650"
                     /old_locus_tag="pgaptmp_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HGU4197347.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2333..2920
                     /gene="betI"
                     /locus_tag="ACHKNB_RS19655"
                     /old_locus_tag="pgaptmp_003931"
     CDS             2333..2920
                     /gene="betI"
                     /locus_tag="ACHKNB_RS19655"
                     /old_locus_tag="pgaptmp_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HGU4197348.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2934..4406
                     /gene="betB"
                     /locus_tag="ACHKNB_RS19660"
                     /old_locus_tag="pgaptmp_003932"
     CDS             2934..4406
                     /gene="betB"
                     /locus_tag="ACHKNB_RS19660"
                     /old_locus_tag="pgaptmp_003932"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HGU4197349.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQDAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4420..6090
                     /gene="betA"
                     /locus_tag="ACHKNB_RS19665"
                     /old_locus_tag="pgaptmp_003933"
     CDS             4420..6090
                     /gene="betA"
                     /locus_tag="ACHKNB_RS19665"
                     /old_locus_tag="pgaptmp_003933"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HGU4197350.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6303..6971
                     /locus_tag="ACHKNB_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /pseudo
     CDS             6303..6971
                     /locus_tag="ACHKNB_RS19670"
                     /old_locus_tag="pgaptmp_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7214..7909)
                     /locus_tag="ACHKNB_RS19675"
                     /old_locus_tag="pgaptmp_003935"
     CDS             complement(7214..7909)
                     /locus_tag="ACHKNB_RS19675"
                     /old_locus_tag="pgaptmp_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HGU4197351.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7902..9329)
                     /locus_tag="ACHKNB_RS19680"
                     /old_locus_tag="pgaptmp_003936"
     CDS             complement(7902..9329)
                     /locus_tag="ACHKNB_RS19680"
                     /old_locus_tag="pgaptmp_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HGU4197352.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9340..10059)
                     /locus_tag="ACHKNB_RS19685"
                     /old_locus_tag="pgaptmp_003937"
     CDS             complement(9340..10059)
                     /locus_tag="ACHKNB_RS19685"
                     /old_locus_tag="pgaptmp_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HGU4197353.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10586..11440)
                     /gene="rclR"
                     /locus_tag="ACHKNB_RS19690"
                     /old_locus_tag="pgaptmp_003938"
     CDS             complement(10586..11440)
                     /gene="rclR"
                     /locus_tag="ACHKNB_RS19690"
                     /old_locus_tag="pgaptmp_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HGU4197354.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         2941 a         3089 c         2828 g         2642 t
ORIGIN      
        1 gctaatacaa aataaaatcg ttaattaata aaatataaag tcatgaagtg atacaagagg
       61 aatttcagga tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc
      121 aatgattccc gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc
      181 gggaacatca cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc
      241 tcgtactggt caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg
      301 ccctggctgc cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt
      361 gcgcggtagg taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt
      421 tgttcttcgc ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc
      481 ggcaggcttt ttagctccac gtacgcgccg cgcaaccgca gctcctgcgc cacttcttcc
      541 attgccgggt aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc
      601 atcaggcgcg agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggc
      661 gcggtatcgc ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac
      721 cccgccatca cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt
      781 tgcagcgcgg atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc
      841 gaccagaaga cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg
      901 aaattcccca gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta
      961 atggtggcga cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtagaag
     1021 ccgcgctccg gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca
     1081 tacagcgcgc tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc
     1141 aacgtgccca gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag
     1201 ccgacaaacg gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc
     1261 cactcaactg gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca
     1321 tagtcgccaa cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat
     1381 acgaacagga tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc
     1441 ttatcgacac cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc
     1501 gcttttgccg ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc
     1561 accacaccga taccgagcgt agtggcaata ccgaagatgg tgccgatcac cgctgcaata
     1621 tccactgagt gccctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag
     1681 cggatggtga gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc
     1741 gcatacatcg accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc
     1801 gcggcctcaa tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg
     1861 gctacggaga agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa
     1921 ctcagcaggc tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc
     1981 gaacaagcga tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa
     2041 ccgaaggttt tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga
     2101 aacaggatcg ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc
     2161 ggattgattt tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt
     2221 tattgttacc atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc
     2281 aatcaataac cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa
     2341 attggggatg cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa
     2401 tgaagtgggc atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac
     2461 ggggatcatc agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga
     2521 tatcaccagt cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag
     2581 tgcagagcag cgattacagg cgattgttgg cggaaacttc gatgaaacgc aggtgagcag
     2641 tgcggcgatg aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta
     2701 tcgtttacag caggtcagta gccgccgctt attgtcgaat ctggtgagcg aatttcgccg
     2761 cgagttaccg cgcgaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg
     2821 attatggctg cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct
     2881 gacccgtcac tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc
     2941 gaatggcaga acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca
     3001 ccttcgagac cattaacccg gccaacggta acgtgctggc gaccgtgcag gacgccgggc
     3061 gcgaggatgt cgatcgcgca gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga
     3121 tgaccgcgat ggagcgctcg cgcattctgc gtcgggccgt cgatattctg cgtgaacgca
     3181 atgacgaact cgcaaaactt gaaaccctcg acaccggaaa agcatattcg gaaacctcaa
     3241 ccgtcgatat cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggcgc
     3301 tggaaggcag ccagatcccg ttgcgtgaga cgtcatttgt ttatacccgc cgcgaaccgc
     3361 tgggtgtggt ggcggggatt ggcgcatgga actacccgat ccagattgcc ctgtggaaat
     3421 ccgccccggc gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc
     3481 ttaccgcgtt aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta
     3541 acgtgttgcc gggggtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg
     3601 ccaaagtgtc atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg
     3661 cctcttccct gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg
     3721 acgatgcgga tctcgatctc gccgccgata tcgccatgat ggcgaacttc ttcagctccg
     3781 gtcaggtgtg taccaacggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg
     3841 agcagaaaat tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa
     3901 ctaacttcgg cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca
     3961 aaggcaaaga ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct
     4021 tcgataacgg cgcatgggtt gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca
     4081 tcgtgcgcga agagatcttc ggtccggtga tgtcgcttct cacttatgaa tctgaggacg
     4141 aagtgattcg ccgcgccaat gataccgact acggcctggc ggcgggcatc gtgacggcgg
     4201 acctgaaccg cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca
     4261 cctggggcga atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc
     4321 gcgagaacgg cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga
     4381 tggctaaatt ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt
     4441 attggtgccg gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc
     4501 tccgtgctgc tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg
     4561 cccgcagcgc tggcgttccc actacagggc aaacgctaca actgggccta tgaaacagag
     4621 cctgaaccgt ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga
     4681 tcgtcgctga tcaacggcat gtgctacatc cgtggcaatg cgctggatct cgataactgg
     4741 gcgcaagaac ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgcaag
     4801 gccgagactc gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc
     4861 accacctcca aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag
     4921 gcaggctacc cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg
     4981 gatcgcaccg tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag
     5041 gccaaatcgc gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt
     5101 gacggcaaac gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc
     5161 gcaacggcca acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg
     5221 caacgctccg gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat
     5281 gaattacccg gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag
     5341 tgcaaagaac cggtttccct ctaccctgcc ctgcagtggt ggaaccaacc gaaaatcggt
     5401 gcggagtggc tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggtgga
     5461 tttattcgca gccgtgagga atttgcgtgg ccgaatattc agtaccattt cctgccagta
     5521 gcgattaact ataacggctc gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc
     5581 tcaatgcgct cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat
     5641 ccggcgattc tgtttaacta catgtcgcac gagcaggact ggcaggagtt ccgcgacgca
     5701 attcgcatca cccgcgagat catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa
     5761 atcagccccg gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac
     5821 gccgaaaccg ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg
     5881 gttgacggcg aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt
     5941 atgccgcaga ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata
     6001 gcggatatga ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca
     6061 aatgggatgc cggtgagagc gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat
     6121 cagattgaga gttaccgttc cagagagggg gaccgaatcc ttatataaac actgaggtaa
     6181 ctctcatgca tcatatccag tattcatgat gctggctttt tgctgatatt taacccgaac
     6241 attcctgata cagactatta atacaacctt atctgcgaca gattttattt tctggatata
     6301 ttatgcgtga acaaatcaaa caggatatcg atctgattga gattttattt tatctgaaga
     6361 aaaaggttcg tgttatcctt tttattatag ctatctgtat ggcaatgatg ctgttgtttc
     6421 tgtatatcaa taaagataat ataaaagtga gttacagcct aaaaataaac cagacaacac
     6481 caagcatact tgttaactgt gatagcaata ataactttgc ctgtcagacc gcaatgactg
     6541 aagatgttat acagcgaatt actacatttt ttcacaccag cccagatgtc aagaacagag
     6601 aaataaagct ggaatggtca tgagataaga gagatttacc aactgctgaa gcggaaatat
     6661 ctcgcgtgca ggcgtctatt atcaaatggt atgcgtcaga ataccataat ggcaggcaag
     6721 ttctcgatga gatacaaata ccttcagcaa ttaacagtga gctttataca aaaatgatat
     6781 acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact ataagctcac
     6841 cagaaataaa aaataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca
     6901 ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact
     6961 ctgggcagta atacacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg
     7021 accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt aaatatcctg
     7081 gcattactaa tgccaggata tttacagaag agcagcataa ccatgacgga acggggttaa
     7141 gtcaggttgt ccttaacgct attttcaacc tcgtatgcct tcttcaggtt tatgttcaga
     7201 tctcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc gggccatgaa
     7261 ccccgacgac tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa
     7321 tgcaggacgg cattcgttca ccggcctgcg ctttctgatg caatttttct gcgagttgcg
     7381 ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagattcc gggagcaggc
     7441 tcaatgaacg cccgcgcccg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
     7501 catattcagc atacacgaca cccactttag cctgctcggc ctgcgagata ttctcggcac
     7561 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttca ctaatcccca
     7621 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
     7681 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa
     7741 catcgctggc aaactgaata aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc
     7801 gctcgttagc atagttgtta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
     7861 gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc
     7921 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
     7981 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
     8041 aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta
     8101 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca
     8161 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac cggacacacg
     8221 ctgtcacaag cagtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
     8281 ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
     8341 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc
     8401 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
     8461 tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg
     8521 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
     8581 tcggcaaacg tgggggcaat acgctccatt cccatcactg caatatgcgt tttaggcagc
     8641 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc tgtctctgcc
     8701 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
     8761 tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttcg
     8821 tgtagcactc gacggatctg atggcgatct ttatgaattg ccgggaccac aacatgagat
     8881 ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatg
     8941 ccagcatcct gcaacacatg attgacccca atctcttcgg tcaccatcga tttagatttc
     9001 accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct
     9061 ttggtttttg caaaccagac gtgaccgccg ttttgcgtca ctttttctga gagctggtac
     9121 agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac
     9181 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
     9241 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
     9301 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc
     9361 agcaatatgc atcactttga ctttctgccc ttcccgttgt aatcgcccac tgatgttcag
     9421 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcacaac
     9481 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
     9541 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
     9601 cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata
     9661 cacagctctc ccttgcaaac tggcacctac atcgactacc cctaatttat taacaataaa
     9721 agaggtgaga tcctgcatac gcgcggcaac cttttcggca cgtgatgccc attcaggttc
     9781 atccgccaga tacgtcgggt aactttttac ggcataggtg caagagccag ccggtgaaat
     9841 aatgggatcg tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
     9901 tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
     9961 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
    10021 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
    10081 tgcccattta tgcccgatgt ttccccccca ccgggggatc aatcatcaat aaatatggcg
    10141 ttctttttta attggctagc aacataccac ccctcatttg tatgacaaat gataatcttt
    10201 catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
    10261 gggaaatcga cgttcatttt tggcataaga aatccattta aaaaggctac atcccttata
    10321 cctattcaga gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa
    10381 tcaccgacaa taatcacgta tcaagtaata gtcggggttt tatttaattg aagcaattta
    10441 gtttgcattt ttatttaaaa actgagctag agtccatcag aaaaaaatat ataaagccca
    10501 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
    10561 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
    10621 ctcccggagt acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat
    10681 agcctactga ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg
    10741 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
    10801 aaagctgggc aaaacttgcc cgggacatgt gagcgatgct ggccagcgat tcgacggtcc
    10861 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
    10921 gcaaactgag aatgtttttc tcagtattaa cctgtgcaat ccactcacgc accgcgaggg
    10981 taaagaatgt agcgcagatc tggctacaca gtgcatccac tcccggcatc gccgatctgg
    11041 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
    11101 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
    11161 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
    11221 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg
    11281 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
    11341 cggcaccatg tggaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
    11401 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
    11461 ttgattacaa acaccaacaa tcttagtaaa taactaatct
//
LOCUS       DAZFVO020000149        11484 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFVO020000149 DAZFVO020000000
VERSION     DAZFVO020000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.150"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHKNB_RS19695"
                     /old_locus_tag="pgaptmp_003939"
     CDS             complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHKNB_RS19695"
                     /old_locus_tag="pgaptmp_003939"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HGU4197355.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1632..2303
                     /gene="cecR"
                     /locus_tag="ACHKNB_RS19700"
                     /old_locus_tag="pgaptmp_003940"
     CDS             1632..2303
                     /gene="cecR"
                     /locus_tag="ACHKNB_RS19700"
                     /old_locus_tag="pgaptmp_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HGU4197356.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHKNB_RS19705"
                     /old_locus_tag="pgaptmp_003941"
     CDS             2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHKNB_RS19705"
                     /old_locus_tag="pgaptmp_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HGU4197357.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHKNB_RS19710"
                     /old_locus_tag="pgaptmp_003942"
     CDS             3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHKNB_RS19710"
                     /old_locus_tag="pgaptmp_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGU4197358.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKAHQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHKNB_RS19715"
                     /old_locus_tag="pgaptmp_003943"
     CDS             5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHKNB_RS19715"
                     /old_locus_tag="pgaptmp_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4197359.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHKNB_RS19720"
                     /old_locus_tag="pgaptmp_003944"
     CDS             6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHKNB_RS19720"
                     /old_locus_tag="pgaptmp_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGU4197360.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHKNB_RS19725"
                     /old_locus_tag="pgaptmp_003945"
     CDS             complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHKNB_RS19725"
                     /old_locus_tag="pgaptmp_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HGU4197361.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHKNB_RS19730"
                     /old_locus_tag="pgaptmp_003946"
     CDS             7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHKNB_RS19730"
                     /old_locus_tag="pgaptmp_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4197362.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8536..9777
                     /gene="clsB"
                     /locus_tag="ACHKNB_RS19735"
                     /old_locus_tag="pgaptmp_003947"
     CDS             8536..9777
                     /gene="clsB"
                     /locus_tag="ACHKNB_RS19735"
                     /old_locus_tag="pgaptmp_003947"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HGU4197363.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHKNB_RS19740"
                     /old_locus_tag="pgaptmp_003948"
     CDS             9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHKNB_RS19740"
                     /old_locus_tag="pgaptmp_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HGU4197364.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHKNB_RS19745"
                     /old_locus_tag="pgaptmp_003949"
     CDS             complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHKNB_RS19745"
                     /old_locus_tag="pgaptmp_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGU4197365.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         2650 a         3053 c         3115 g         2666 t
ORIGIN      
        1 tcatcagcct gatgccgggc atagcccggc ataaaagatt actgcgcagc ggtaggttta
       61 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac
      121 ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg gcgtggttgc
      181 tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccgttc
      241 tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg
      301 atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc
      361 agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg gtggacataa
      421 tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga
      481 ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cacccgattt aaaatcagcc
      541 agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact acggatgcca
      601 tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt aaacaccagc
      661 acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt gcgtttctta
      721 tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg
      781 atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc
      841 gcagagaaca gcaggttctg gcgtttcgct ggcagttttg ttaacacgcg acggatatcg
      901 tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga
      961 tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt tgccaccaga
     1021 acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc gccaaacacc
     1081 accagcgaac gaatgttcaa atatttgctg taatcacgga cgttttcgcc aatctgcgca
     1141 gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc
     1201 ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttaccggta
     1261 ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc
     1321 tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt
     1381 aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt
     1441 tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt
     1501 gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct
     1561 tagccatact ggacaactcc cctatggagt cataatctta atcaatcatt tgattaagaa
     1621 tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc
     1681 tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga
     1741 tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag
     1801 atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc
     1861 acgccgaaga ggccgaacgc ttgttcgcac aaccacagcc tgatcgcgca gccatccgtg
     1921 aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca
     1981 acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctat cacctggtgc
     2041 acgaacaggt gattagcccg ctacacagcc acctgacacg cctgattgcc gcctggaccg
     2101 gctgcgacgc caacgatacc cgcatgatcc tccatactca tgcgttgatt ggtgagattc
     2161 tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg
     2221 aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag
     2281 gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggat tggcggtagt
     2341 ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg
     2401 cctgacgctg tatggcaacg tggatattcg tacggtaaat cttagtttcc gtgttggggg
     2461 gcgcgttgaa tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc aggtgctggg
     2521 cgaactggat cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gcgtctcggt
     2581 ggcacaggcg cagtatgatc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc
     2641 cgccgcagcg gtgaaacagg ctcaagccgc ctatgactat gcgcagaact tctataaccg
     2701 ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc
     2761 ctcgcgcgac caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg
     2821 ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc agcctcgaac aggcgcaggc
     2881 gcaactggcg caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg
     2941 cacgctgtta acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt
     3001 taccgtttca ctaacgcgtc cggtgtgggt acgtgcctat gttgatgaac ggaatcttga
     3061 ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta
     3121 tcacggacag attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac
     3181 accggatctg cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga
     3241 tgcgttacgc cagggaatgc cggtgacggt gcaattcggt aacgaggcag gacatgaatg
     3301 atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg
     3361 tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggctggtg gggccggacg
     3421 gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt actgaaaccc gacagcggca
     3481 atgccacggt gattggcttt gatccaatca aaaacgacgg cgcgctgcac gccgtgctcg
     3541 gctatatgcc gcagaaattt ggtctgtatg aagatctcac ggtgatggag aacctcaatc
     3601 tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc
     3661 tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc tccggtggga
     3721 tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg
     3781 atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg
     3841 agctggcggg cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc
     3901 agtgccgtga cgtgttactg atgaacgaag gcgagttgct gtatcaggga gaaccaaaag
     3961 ccctgacaca aaccatggcc ggacgcagct ttctgatgac cagtccacac gagggcaacc
     4021 gcaaactgtt gcaacgcgcc ttgaaactgc cgcaggtcag cgacggcatg attcagggga
     4081 aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgaag
     4141 ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgcg tttattgatt
     4201 tgctgggcgg tgccggaacc tcggaatcgc cgctgggtgc aatattgcat acggtagaag
     4261 gcacacccgg cgagacggtg atcgaagcga aagaactaac caagaaattt ggtgattttg
     4321 ccgccaccga tcacgtcaac tttgccgtta aacgagggga gatttttggt ctgctcgggc
     4381 caaacggcgc ggggaaatcg accaccttta agatgatgtg cggcttgctg gtgccgacct
     4441 ccggtcaggc gctggtgctg gggatggatc tgaaagagag ttccggtaaa gcgcaccagc
     4501 atctcggcta tatggcgcaa aagttttcgc tctatggcaa cctgacggtc gaacagaatt
     4561 tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc
     4621 gtatgagcga ggcgttcggc ctgaaaagta ttgcctcaca cgccaccgat gaactgccat
     4681 taggttttaa acagcggctg gcgctggcct gttcgctgat gcatgaaccg gatattctgt
     4741 ttctcgacga accgacttcc ggcgttgacc ccctcacccg ccgtgaattt tggctgcaca
     4801 tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag
     4861 cggaatattg cgaccgcatc ggcctggtgt accgcgggaa attaatcgcc agcggcacgc
     4921 cggacgattt gaaagcacag tcggctaacg atgagcaacc cgatcccacc atggagcaag
     4981 cctttattca gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt
     5041 tcctggcgtc gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg
     5101 agtagttggc tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc
     5161 attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg
     5221 gcgctggatt tcacccacac catgaccggt tcgccctaca tcgacgccac catcagcgat
     5281 aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt
     5341 ccggtggatt ttgcggaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc
     5401 accgacggca gtgagccgaa taccgctaac tttgtacagg ggtatgtcga agggatctgg
     5461 cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat
     5521 gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt
     5581 gcggtgacca ttatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga
     5641 gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg
     5701 ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg
     5761 ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt cgctgctgat tctgtttttt
     5821 atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc
     5881 cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg cttttctgcc gtcgattatg
     5941 ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac
     6001 attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt
     6061 ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc
     6121 ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt
     6181 atggacgtta atccgcaaag agttgcagtc gctgctgcgc gaaccgcaaa cccgcgcgat
     6241 tctgatttta cctgtgctta tacaggtgat tctgttcccg ttcgccgcca cgctggaagt
     6301 gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac
     6361 ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga
     6421 gatccgccca accatcgaca cacaaaaggc gttactactg gtgcgtttcc cggctgactt
     6481 ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa
     6541 ctccaacagt gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca
     6601 ggagctgctg gaaggaaaac cgaaacctaa caacagcgag ctggtggtac gcaactggta
     6661 taacccgaat cttgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac
     6721 tattggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct
     6781 cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc
     6841 ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta
     6901 tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga tttacggttt
     6961 atcgctggtg ggattcggtc tgttgatttc atcactctgc tcgacacaac agcaggcgtt
     7021 tatcggcgtg tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt
     7081 ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac
     7141 ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt
     7201 gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg
     7261 taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga
     7321 ttgccagccc tgcaattgcc agcaaaaagt tgttttccag tacacgggac aacagccaca
     7381 gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca
     7441 cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact accgtgacac
     7501 aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca
     7561 ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga ccagttgcga
     7621 cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc
     7681 agagtaccga actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca
     7741 ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt
     7801 ttcgttcaag gtgctcacca tcaacattca caaaggtttt accgcgttta accgacgctt
     7861 cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg tttgcctgca
     7921 ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc
     7981 gcactacgag tttctcgccg acacgatgtg gagcgatttt gcctacggtc gcaatgccgt
     8041 atacccggaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga
     8101 gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt
     8161 gccaccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc
     8221 gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga
     8281 accggtattg gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa
     8341 agtgcaggcc ggactggatg agatttttac ccgcgtccac ggacgcccgg cgcgcacgtt
     8401 tccggtgcaa tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag
     8461 cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag
     8521 tgcggagatt catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa
     8581 aacggcgagc aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc
     8641 attcttgaaa cgtttatctg gtttgaggat gacgtcggca aacagctgca tgcggcacta
     8701 ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg
     8761 gatctcagcg atgagtttgt caatgaactg acggcagctg gcgtggtatt tcgctactac
     8821 gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa
     8881 attgtggtga tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat
     8941 atgtccagct acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt
     9001 gtcgaagata ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc
     9061 tggtggcgac gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg
     9121 ctggtctggc gcgataacga agaacatcgc gatgatattg aacgtcacta tctgaaaatg
     9181 ctcactcagg cgcggcgaga agtcattatc gccaacgcct acttcttccc cggctatcga
     9241 tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag
     9301 ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt
     9361 aaaggcggcg ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg
     9421 atggacgatc actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg
     9481 aatctcgaag caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat
     9541 ctgaacggca ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc
     9601 acctggtgga acctgaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg
     9661 ctggttggct ggcttccggc acacacacca cgtctggcgc aggttgatcc gcccgcacaa
     9721 ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga
     9781 gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct cacctggctg ttttttatcg
     9841 cggtgattgt gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg
     9901 tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct
     9961 atctgattta cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga
    10021 agcgccaggt gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct
    10081 gggtcggcgg cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca
    10141 ctattacgcg gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg
    10201 cagggattat ctttaccgca ggcgtggtgg agttgccgga tcactggtat gtcgatcaaa
    10261 ctacgctgcg tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt
    10321 gcgctttcgc gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcat
    10381 ggaaattcgc cctcgctcag atgctgattt ccagcgttaa ctggatggta atgggggcga
    10441 ttatctggct gttacttggt caaagcgtga actatttctt tgtactgggc gtgttactgg
    10501 ttagtagtat tgctggcgtc atcgtgcata ttccggcggg gatcggtgtg ctggaagcgg
    10561 tgtttatcgc actactggct ggggagcata cctcgaaagg gagcattatc gccgccctgc
    10621 tcgcttatcg cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc
    10681 tactggaaag ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaagtttag
    10741 ttaagttctt tagtatctgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga
    10801 caagcgttat agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg
    10861 ttaatgcacc tgtaacagcg caacgaaccg gactccacaa ctcttcacta tggcatttcc
    10921 ccgccaattc aaggagctta tttcgtccat gcgagataat gcctgaccat aacacaatca
    10981 ttaagcaact tattacccaa acgggtcgtt cactcattaa tgcggcattc atgatgatca
    11041 ccagggccaa ccctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat
    11101 taaataataa accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga
    11161 cgataatggc aggtacagga gctaaacttg caacgacaat cgccaccaaa gagagtaaaa
    11221 acagatatat tacgcctgcg atgacggcat ttatcttgtt gatatcctta cttaagccat
    11281 acatacaaac taataataac cctatggcga catacattgt tggtgaatca tatttggttg
    11341 ccgtaatttc gagatactta gctccccagg ccagtcctgc ggttgccacc aaacctaaag
    11401 cggtaatgag ccatataccg tttaatatcc tggtctcgtg ctgaaaatct aatttattgc
    11461 tgttttgtga gtatgattcc attc
//
LOCUS       DAZFVO020000150        11470 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFVO020000150 DAZFVO020000000
VERSION     DAZFVO020000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.151"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            340..1206
                     /gene="folD"
                     /locus_tag="ACHKNB_RS19750"
                     /old_locus_tag="pgaptmp_003950"
     CDS             340..1206
                     /gene="folD"
                     /locus_tag="ACHKNB_RS19750"
                     /old_locus_tag="pgaptmp_003950"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HGU4197366.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1208..1420
                     /gene="ybcJ"
                     /locus_tag="ACHKNB_RS19755"
                     /old_locus_tag="pgaptmp_003951"
     CDS             1208..1420
                     /gene="ybcJ"
                     /locus_tag="ACHKNB_RS19755"
                     /old_locus_tag="pgaptmp_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HGU4197367.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1528..2049
                     /locus_tag="ACHKNB_RS19760"
                     /old_locus_tag="pgaptmp_003952"
     CDS             1528..2049
                     /locus_tag="ACHKNB_RS19760"
                     /old_locus_tag="pgaptmp_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4197368.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2085..3470)
                     /gene="cysS"
                     /locus_tag="ACHKNB_RS19765"
                     /old_locus_tag="pgaptmp_003953"
     CDS             complement(2085..3470)
                     /gene="cysS"
                     /locus_tag="ACHKNB_RS19765"
                     /old_locus_tag="pgaptmp_003953"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGU4197369.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3644..4138
                     /gene="ppiB"
                     /locus_tag="ACHKNB_RS19770"
                     /old_locus_tag="pgaptmp_003954"
     CDS             3644..4138
                     /gene="ppiB"
                     /locus_tag="ACHKNB_RS19770"
                     /old_locus_tag="pgaptmp_003954"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HGU4197370.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4141..4863
                     /gene="lpxH"
                     /locus_tag="ACHKNB_RS19775"
                     /old_locus_tag="pgaptmp_003955"
     CDS             4141..4863
                     /gene="lpxH"
                     /locus_tag="ACHKNB_RS19775"
                     /old_locus_tag="pgaptmp_003955"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HGU4197371.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4981..5490
                     /gene="purE"
                     /locus_tag="ACHKNB_RS19780"
                     /old_locus_tag="pgaptmp_003956"
     CDS             4981..5490
                     /gene="purE"
                     /locus_tag="ACHKNB_RS19780"
                     /old_locus_tag="pgaptmp_003956"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGU4197372.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5487..6554
                     /gene="purK"
                     /locus_tag="ACHKNB_RS19785"
                     /old_locus_tag="pgaptmp_003957"
     CDS             5487..6554
                     /gene="purK"
                     /locus_tag="ACHKNB_RS19785"
                     /old_locus_tag="pgaptmp_003957"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGU4197373.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6691..7584)
                     /locus_tag="ACHKNB_RS19790"
                     /old_locus_tag="pgaptmp_003958"
     CDS             complement(6691..7584)
                     /locus_tag="ACHKNB_RS19790"
                     /old_locus_tag="pgaptmp_003958"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGU4197374.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQRLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSIGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7581..8396)
                     /locus_tag="ACHKNB_RS19795"
                     /old_locus_tag="pgaptmp_003959"
     CDS             complement(7581..8396)
                     /locus_tag="ACHKNB_RS19795"
                     /old_locus_tag="pgaptmp_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HGU4197375.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8407..9666)
                     /locus_tag="ACHKNB_RS19800"
                     /old_locus_tag="pgaptmp_003960"
     CDS             complement(8407..9666)
                     /locus_tag="ACHKNB_RS19800"
                     /old_locus_tag="pgaptmp_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HGU4197376.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGSGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9676..11343)
                     /locus_tag="ACHKNB_RS19805"
                     /old_locus_tag="pgaptmp_003961"
     CDS             complement(9676..11343)
                     /locus_tag="ACHKNB_RS19805"
                     /old_locus_tag="pgaptmp_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HGU4197377.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2567 a         3264 c         3023 g         2616 t
ORIGIN      
        1 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
       61 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
      121 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg gacgaaaaat
      181 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
      241 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aataggcgca
      301 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
      361 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggcgcgtatt
      421 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
      481 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
      541 tatgacctcc cggaaaccac cagcgaagcg gagctgttgg agcttatcga tgcgctgaat
      601 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
      661 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttataac
      721 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
      781 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
      841 tcgaatatcg tcggtcgtcc aatgagcatg gaactgttgc tcgcaggttg caccactacc
      901 gtgactcacc gtttcactaa aaatctgcgt caccacgtag aaaatgccga tctgttgatc
      961 gttgccgtcg gcaagccggg ctttattcct ggtgactgga tcaaagaagg cgcaattgtg
     1021 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     1081 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     1141 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacaggat
     1201 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     1261 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     1321 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     1381 agtgagtttt gcaggtcaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     1441 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     1501 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgctgttccc
     1561 ctatgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     1621 atcatcctgg cgatgctgcc cgacgccgac gtattgtcgt ttaaatttgg cgttgcttac
     1681 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     1741 ctgttatgtg tgctgattgg acgccgatgg ttcagggctg ggctgattcg ctgctggctg
     1801 tttttaaccg tctcgttgct atcgcacagc ttgctggatt cggtaaccac tggcggtaag
     1861 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     1921 aaagtcgcgc cgtttgcatt gtctcgttac accacgccgt acgggcatca ggtgataatt
     1981 tctgaattga tgtgggtgtg gttgccgggg atggtgctta tgggaatgtt gtggtggcgc
     2041 aggcgataac cggatgcgaa aactcgcatc cggcaacagc gcaattactt acgacgccag
     2101 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgcgcc
     2161 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     2221 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     2281 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     2341 gccgctgcca tatcttctgc tttcagacgg tttacttcac gcgccatatc aaacagtacg
     2401 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     2461 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagagacgc
     2521 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcaactg gctgcgatag
     2581 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     2641 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccatcccc
     2701 gagtgcatcc agtagttcac atactggcca tcatgggcac aggtggactg cgcgatttcg
     2761 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     2821 agctgcttgc agttcattgc cgaacattca atgtgccagc ctggacggcc cgcgccccac
     2881 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     2941 ttgcgtttgt cgtcgaccac gtcaacgcgc gcacctgcct gcagctggtc gagatcctga
     3001 cgcgacagca cgccatatgt cggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     3061 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     3121 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     3181 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     3241 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     3301 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     3361 tgacagagat cgtaaacggt gattccacac acatacatgc cgacttcccc ggcgtgaata
     3421 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     3481 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     3541 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     3601 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     3661 caatcacggc gatattgtca tcaaaacttt tgacgataaa gcacctgaaa cagttaaaaa
     3721 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     3781 cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga
     3841 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     3901 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     3961 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     4021 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtagcggtat
     4081 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     4141 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     4201 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     4261 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     4321 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     4381 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     4441 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     4501 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     4561 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg agcgaacagt
     4621 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgcggtagtc
     4681 agtgcgatgg aaaaacatca ggtgcaatgg ctgatccacg gacataccca tcgcccggcg
     4741 gtacatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactcgg tgcctggcat
     4801 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     4861 taaaaaaccc gcaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     4921 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     4981 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     5041 gctaccatgc agttcgccgc cgaaatcttc gaaatcctga atgtcccgta ccacgttgaa
     5101 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     5161 aacggttatc aggtgattat tgcgggcgca ggtggcgcag cgcacctgcc aggcatgatt
     5221 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     5281 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     5341 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     5401 gataaagagc tacaccagcg tctgaatgac tggcgcaaag cccagaccga cgaagtgctg
     5461 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     5521 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     5581 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     5641 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     5701 atgtgttccc gattattgct gaccgtctga ctcagaagca gcttttcgat aagctccacc
     5761 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     5821 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     5881 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggc gaatgtattg
     5941 tcgagcaggg cattaacttc tctggtgaag tgtcgctggt tggcgcgcgc ggctttgatg
     6001 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     6061 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     6121 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     6181 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     6241 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     6301 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgcgaatt
     6361 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     6421 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     6481 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     6541 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     6601 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     6661 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     6721 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     6781 gacataacca ctcaccgccg ttaccttcgg cccgatcgaa ccatcggctt tggcaaatgg
     6841 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     6901 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     6961 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     7021 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     7081 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc gcaagtattt
     7141 accatcacgt ttcatctgcc agccgtaagc tgcttccagt gcctcttgtt cttctggctg
     7201 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     7261 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca acctctgcgc
     7321 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     7381 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     7441 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     7501 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     7561 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     7621 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     7681 gcggtacgtt tcgggcaact cagagcatga acaaagtgca ggagtggcga ggcgaaatat
     7741 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     7801 tcggaacagg caaaaaacgg gcgaccagag cgcgcatcta aagcgccata ataccaggcc
     7861 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg tcctttaccc
     7921 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
     7981 tggcgtaatt cagcggtcag cggatcgctt gccgccaacg ccagtggccc gaaaagcccg
     8041 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcgc aggcggcgta
     8101 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
     8161 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
     8221 gcgcggcgaa gcacccatcc tccggggccg aaaccactac cctgacgatg cagcgttaac
     8281 agttcgccgc tttccgtcat caggttaatc gcccgccgcc acactcccgc taaccgccag
     8341 gactggcgat aattcggtgc actacggctg gcaagcagag gatggatgat cgtcattgcg
     8401 cgttcctcaa ccaatcccca tgctttcagc cagtgcttcc agcgcctgtt caaaacacgc
     8461 cagcggtgcc cgcacagtgc ctgcgccaat ctgcccgctc cccgcctctt tatgggcgat
     8521 accggtattg atgagtggcg taatgccggt ttctaccacg cgacgaatgt ccagtcccag
     8581 gcacgcgccc tgaaaatccc agcctgggat ctgcaactgc atattgcgct caaggtaaat
     8641 ttccgccatc tcttcagata ccgctctggc cgcttccatg ccacccgcac cgacaaagcg
     8701 cgttacgcca ggcgctgcga tcattgccgc acctccgata ccaaaggttt cggtaatcgc
     8761 gctatcgccc atatccgggt tcgcctgctc ctgcgagaag ccggtgaaaa acagaccttg
     8821 cggagtgttt acaggcgcag taaaccagcg ttcgcccagc ccgcttaccc gaatcccgaa
     8881 catattgccg ttgcgggtca ttgccgtgac gatgctgcct gcgcggatca tcgcgccagc
     8941 atccatcgcc gccttgcagt aagccatcgc gaggttgagg aagaactgat cggtcacgct
     9001 gaggaaatcc atcacttcgg cgatgtgctg tttatcatga tcgaggcgag caatttgtgg
     9061 ggccagcgca cgcattaaca gtgcagagga agcaatattg cgttgatgga actcatcgcc
     9121 catcgtaatg ccctgcgcca tcatcgccgt gagatcgata ccgcgctcca tgcgccccag
     9181 cgccgcgctt aatactggca ttaacacatc gcgcatccag cgatggcgag tcaggacatc
     9241 ttcgccgtaa gcgccaaaac gcatcacttt gccgatacct tcgttgaggt tgcagtacgc
     9301 ccggttgccg tcggtcacgt tctcgaccac cagcatcggc atactggcag aagtaatacc
     9361 gcccattgga ccgacggcat tcacatggtg acaaggaatg aagttcactt ccccctgctc
     9421 cagtattgcc agcgcctgcg cttcatcttt cgcccagcct tcgaacagac atgcgcccac
     9481 gcacgcccct ttcatgggtc cggtcatctc ctgccagcgc attggcggcc cggcgtgaag
     9541 cagtgttttg ccctcgttta gttcgctgat aagtgaagaa gccggttgca catccagcca
     9601 gtgtggacga gcacgacgaa tttgttcgat aaccgcagca ttggcttgcg ccactgatgt
     9661 aaacatggga accccttatt gcaaacgttc taataaacga gccagttttt tattgccgcc
     9721 ggcgactggc gaccattggt aatgcacaac cggtttgctg gcgctttgta gctccagcgc
     9781 aaagctgcgt aatccgatgt taatcacggc gacgttttcc agtaatgacg gtgtgtgttg
     9841 ctgagtggca ggcgaaagcg gacgaatcag tgccgccgcc agcaaggtgg cttccggcag
     9901 cgaactcact accgcgatcc ctgcatcttc cagcgtggcg atttgctgcg agcggcattg
     9961 cgggtcacgt tcagtgcctg tcaccgtggc aatggcatac agtggttgat tgtctgaacg
    10021 cgcggcacat gctttttgcc aggcgcttac cagcgaggcg gcaggatcgg cggtcgcacc
    10081 gaagccaatc acgacatcaa gcagcaatac gcgcacttgc ggtttagcgc cgagatcggc
    10141 aattaactga ttgcgtaagg ctgggtcgat catcggatgg ggacgcccga cggtgtagaa
    10201 atcatcgccg aggtcaataa tctggtgacc atcggcgtcg agcatcatgc cgtgatgatg
    10261 ggtatcgtcg gcttccacgc caaggtgccc ggcaagtaat cccgccgctt cggcagccag
    10321 cgtaccgccg gtatacaaac cgcaaataaa tccgctgctg gcaggcgtta tcgcgttacg
    10381 tcgcgccgta acgcgtgaaa gcaggcaagc caggcgtgcg gcctcatcca gcgaggaggc
    10441 aaaccagaca ttctcgtcgc gggccaccgc cggggtataa cctaaaaaca gcgccaccgt
    10501 cggtttgccg gttgctttca tggcattaac aattttcaga cgcacagctt cggcaggcgg
    10561 ttttgaaaca aatgccagca cttcgctttt ctcgtctgca ctgagcattt ccagcgccgt
    10621 cagcgcactg atgccgccca cttcacggct gaggtcgcgc ccgccaaggc caatcgcgtg
    10681 agtaattccc tcccctgcca gcgcaatctg tgagcacagc tcctgaatcc cggtaccgga
    10741 agcgccaatg acgccaatgt tgccttccgg catcacgtta gcaaaagcca gcggcgtggc
    10801 ggcaatcatc gacgtaccgc agtccggccc catcaccagc aagccttttt ctcgcgcgcg
    10861 ggttttaagt tggatttcat cttccagcgt gacgttatcg gagaacatca tcacgttgag
    10921 gttgcgatcc agcgcctgat tcgccagctc cgccgcatac tcgccagcca ctgaaatcag
    10981 cgccagattg gcatcggata atttctgaca ggcactgtcc cagcgacgca cctgcgtcaa
    11041 ggactggctg ctgcctgacc cctgcgccag ttgttttagc gcctcttcaa gctgctgcat
    11101 aatcgcctgc gcgatccccg catccgccgc ttcgctacgt attgccacgc aaatatcgtt
    11161 cggcgtggcg ttattaaaat cgtcatgcca gaaacctgtg gtatctaata acgctttatt
    11221 ggcgggcgta cccatcatta cggaaacatc atcaacattt tctgattcgc tgagttttcg
    11281 tgaaataatc attaaactga ccgaatcctg aaaacaccct tttttaataa aggcgtggat
    11341 cataacaact ccttaaaaat tgcacccatg gcaattgcac gagcagtgtt aatgtggcga
    11401 ttacggtatc gatgacaacg gataaataag gtgatgaata tcacacaggg attattaaca
    11461 aaagaattca
//
LOCUS       DAZFVO020000151        11379 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFVO020000151 DAZFVO020000000
VERSION     DAZFVO020000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.152"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..964
                     /locus_tag="ACHKNB_RS19810"
                     /old_locus_tag="pgaptmp_003962"
     CDS             <1..964
                     /locus_tag="ACHKNB_RS19810"
                     /old_locus_tag="pgaptmp_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HGU4197378.1"
                     /translation="KQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSND
                     KDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIVEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1233..3470
                     /gene="nrfB"
                     /locus_tag="ACHKNB_RS19815"
                     /old_locus_tag="pgaptmp_003963"
     CDS             1233..3470
                     /gene="nrfB"
                     /locus_tag="ACHKNB_RS19815"
                     /old_locus_tag="pgaptmp_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HGU4197379.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3457..6429
                     /gene="nfrA"
                     /locus_tag="ACHKNB_RS19820"
                     /old_locus_tag="pgaptmp_003964"
     CDS             3457..6429
                     /gene="nfrA"
                     /locus_tag="ACHKNB_RS19820"
                     /old_locus_tag="pgaptmp_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HGU4197380.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6430..7320
                     /locus_tag="ACHKNB_RS19825"
                     /old_locus_tag="pgaptmp_003965"
     CDS             6430..7320
                     /locus_tag="ACHKNB_RS19825"
                     /old_locus_tag="pgaptmp_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HGU4197381.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7503..8264
                     /gene="envY"
                     /locus_tag="ACHKNB_RS19830"
                     /old_locus_tag="pgaptmp_003966"
     CDS             7503..8264
                     /gene="envY"
                     /locus_tag="ACHKNB_RS19830"
                     /old_locus_tag="pgaptmp_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HGU4197382.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNKNTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8777..9730
                     /gene="ompT"
                     /locus_tag="ACHKNB_RS19835"
                     /old_locus_tag="pgaptmp_003967"
     CDS             8777..9730
                     /gene="ompT"
                     /locus_tag="ACHKNB_RS19835"
                     /old_locus_tag="pgaptmp_003967"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HGU4197383.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9917..>11379
                     /locus_tag="ACHKNB_RS19840"
                     /old_locus_tag="pgaptmp_003968"
     CDS             9917..>11379
                     /locus_tag="ACHKNB_RS19840"
                     /old_locus_tag="pgaptmp_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4197384.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITH
                     FHELLSKLHHKPLLDSKGENISSGELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPG"
BASE COUNT         3105 a         2788 c         2747 g         2739 t
ORIGIN      
        1 gaagcaatat ggtgattttg aaaatggtat tcctgttcac gatactattg ccagagttgt
       61 atcctgtatc agtcctgcaa aatttcatga gtgctttatt aactggatgc gtgactgcca
      121 ttcatcaaat gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga
      181 caagagtcgc cgcaggggag cgattcatgt cattagtgcg ttctcaacaa tgcacagtct
      241 ggtcatcgga cagatcaaga cggatgagaa atctaatgag attacagcta tcccagaact
      301 tcttaacatg ctggatatta aaggaaaaat catcacaact gatgcgatgg gttgccagaa
      361 agatattgta gagaagatac aaaaacaggg aggtgattat ttattcgcgg taaaaggaaa
      421 ccaggggcgg ctaaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc
      481 agagcatgac agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa tccgtcttca
      541 tattgtttgc gatgtccctg atgaacttat tgatttcacg tttgaatgga aaggtctgaa
      601 gaaattatgc gtggcagtct cctttcggtc aataatagca gaacaaaaga aagagcccga
      661 aatgacggtc agatattata tcagttctgc tgatttaacc gcagagaagt tcgccacagc
      721 aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg tggtaatgaa
      781 tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa ttattttcag ggatacggca
      841 catcgctatt aatattttaa cgaatgataa ggtattcaag gcagggttaa gacgtaagat
      901 gcgaaaagca gccatggaca gaaactatct cgcgtcagtc cttgcgggga gcgggctttc
      961 gtaatcttgc cctgcctgct cacctcctcc aggcacttcc actacattat gacctgctcg
     1021 gtgccaccgc tccttttcac tatgacgtta acctcccctc cttgacacca tgcttttgtc
     1081 tacattctac gcaaacggca acttttataa ccagtgtaaa aataacgtgc cgcaatagtc
     1141 atacctatta atggtaaaaa gctgtcacaa ttcataaaaa accttaatat acgccaccct
     1201 aaacataacc agcgttaatg taaggttttt gtgtggactg gcttcttgat gtttttgcaa
     1261 cctggctcta cggcttaaaa gtaatcgcga taacgttagc ggtcatcatg ttcatcagcg
     1321 ggctggacga tttttttatt gatgtcgtct actgggtacg ccgcattaaa cgcaagttga
     1381 gtgtttatcg ccgctacccg cgaatgagtt accgcgaact gtataaacca gatgaaaaac
     1441 cgttagcgat tatggttccg gcgtggaatg aaacgggcgt catcggcaat atggccgagc
     1501 tggcggcgac cacgctcgac tacgaaaact atcatatctt tgttggcacc taccccaacg
     1561 accccgatac tcaacgtgat gttgacgaag tgtgcgctcg ctttccgaac gtgcataagg
     1621 tagtctgcgc gcgtcctggt cccaccagca aagccgactg tctgaacaac gtgctggacg
     1681 ccatcaccca gtttgaacgt agcgccaatt tcgcttttgc tggttttatt ctgcatgacg
     1741 ccgaagatgt gatttcaccg atggaattgc gtctgttcaa ctatctggtc gagcgtaaag
     1801 atctgattca gatcccggtg tatccgttcg aacgcgaatg gacgcacttc accagcatga
     1861 cttacattga tgagttttca gagctgcatg gcaaagatgt tccggtgcgt gaagccctcg
     1921 ccggacaagt gcccagcgca ggcgtcggca cctgtttcag ccgccgcgcc gtgaccgctc
     1981 tgttagctga cggtgacggt attgctttcg acgtgcagag tctgactgaa gattacgaca
     2041 ttggtttccg cctgaaagag aaaggtatga cggaaatttt tgtccgtttt ccggtggtgg
     2101 acgaagccaa agaacgcgag cagcgcaaat ttttacagca cgcacggacg tcaaacatga
     2161 tctgcgtgcg cgaatatttc cccgatacct tttcgactgc agttcgacaa aaatctcgct
     2221 ggatcatcgg cattgttttc cagggcttta aaacccacaa atggacctcc agcctgacgc
     2281 tgaactactt tctctggcgc gaccgcaaag gggcaatcag taactttgtc agcttcctcg
     2341 cgatgctggt gatgctccag cttttgctgt tgctggcgta tgaaagtttg tggcccgatg
     2401 cctggcattt cctttctatt tttagcggca gcgcatggtt aatgaccctg ctgtggctaa
     2461 actttggctt gatggttaac cgcatcgtgc agcgggtgat tttcgtcact ggctactacg
     2521 gtctgacgca ggggctgtta tctgtcctgc gtcttttctg gggcaacctg attaacttca
     2581 tggccaactg gcgcgcgcta aaacaggtac ttcaacacgg cgatccacgt cgcgtcgcgt
     2641 gggataaaac aacgcatgac ttccccagcg tgactggcga tacccgctcg ttgcgcccgt
     2701 taggccaaat tctgctggaa aatcaggtca tcactgaaga acaactcgat acagcactgc
     2761 gtaatcgcgt cgaaggtcta cgcctgggcg gttcaatgct gatgcagggg ctgattagcg
     2821 ccgagcagct ggcacaggcg ctggcagagc aaaacggcgt ggcgtgggaa tccatcgatg
     2881 cctggcagat cccgtcctcg ctgattgccg aaatgccggc ctccgtagcg ctgcattatg
     2941 cagtactgcc gctgcgtctg gaaaatgatg agttaattgt cggcagtgaa gatggtattg
     3001 acccggtttc gctggcggcc ctgacgcgta aagtcggacg caaagtgcgt tacgtcattg
     3061 ttctgcgggg acaaattgtc acggggttac gtcactggta tgcacgccga cgcggtcacg
     3121 atccgcgggc aatgttgtac aatgcggttc agcatcagtg gctcacggaa cagcagaccg
     3181 gtgaaatctg gcggcaatat gtgccgcatc agttcctgtt cgccgaaata ctgaccacgc
     3241 tcggtcatat taatcgttca gcaattaacg tgttgttatt gcgccatgaa cgcagttctc
     3301 tgccgctcgg caagtttttg gtcaccgaag gcgttatcag ccaggaaacg ttggatcgcg
     3361 tcctgacaat tcaacgcgaa ttacaagttt cgatgcaatc actattactc aaagcaggtt
     3421 taaacacaga acaggttgcg caactggagt ccgaaaatga aggagaataa ccttaatcgc
     3481 gtcatcggat ggtctggttt actgctgacg tctttattga gtaccagcgc actcgcagac
     3541 aatatcggca ccagcgcaga agagctgggg ctgagcgatt atcgccattt tgttatttat
     3601 ccccgtctcg ataaggcgct gaaggcacag aaaaataacg acgaagcaac cgccatccgc
     3661 gaatttgaat atatacacca gcaggtgccg gataatattc cgctgacttt ataccttgcg
     3721 gaagcctatc gccattttgg tcatgatgac cgggcgcggc tgttgcttga ggatcaactg
     3781 aaacgtcacc caggagatgc ccgacttgag cgcagtctgg cggctattcc ggttgaagtg
     3841 aaaagcgtta cgactgttga agaactgctt gcccagcaaa aagcgtgcga cgctgcgccg
     3901 accctgcgtt gtcgcagtga agtcgggcag aatgccctgc ggctggcaca attacctgtc
     3961 gccagagcgc aactgaacga tgcgacgttt gctgcatcgc cagaaggaaa aacgctgcga
     4021 accgatctgc tgcaacgggc aatctacctg aaacaatggt cccaggcaga tacgctatac
     4081 aatgaagcac gccagcagaa cacattaagc gcggcagaac gccgtcagtg gtttgacgtg
     4141 cttcttgccg ggcagctgga cgatcggatc ctggcactgc aatcacaggg gatcttcacc
     4201 gatcctcagt catatattac ttacgcgacc gcgctggctt atcgtggcga aaaagcacgc
     4261 ctccagcatt atctcaatga aaataagccg ctgtttacca cggacgcaca agagaaaagt
     4321 tggctctatc tgttatctaa atacagcgcc aaccccgttc aggcattggc gaattatacg
     4381 gtgcagtttg ccgataaccg ccagtatgtt gttggcgcga cgctaccggt gctgttaaaa
     4441 gaaggtcagt acgacgcagc gcaaaaactg ctcgccaccc tccccgccaa tgaaatgctt
     4501 gaggagcgtt atgctgtcag cgtggcgacc cgtaacaagg ctgaatctct gcgtctggca
     4561 cgattgctgt atcagcaaga accggcaaat cttacccgcc tggatcaact gacctggcaa
     4621 ctgatgcaga acgagcagtc acgcgaagct gccgatttat tgctgcaacg ctatcctttc
     4681 cagggcgatg cgcgtgtcag ccagacttta atggcgcgac tggcgtctct gctggaaagt
     4741 catccttacc tggcaacgcc ggcgaaggtg gcgattttat cgaaaccctt accgctggcg
     4801 gagcaacgtc agtggcaaag tcagttgccg ggtattgcag ataattgccc ggcaatagtt
     4861 cgcttgctgg gcgatatgtc gccttcctac gatgcttccg cctggaaccg tctggcaaag
     4921 tgttatcggg acacgctacc cggtgtggcg ctgtatgcat ggcttcaggc cgaacaacga
     4981 caaccgaacg cctggcaaca tcgtgcggta gcctatcagg cgtatcaggt tgaggactac
     5041 gccaccgcac tggcggcctg gcagaaaatc agtcttcacg acatgagcaa tgaggatctg
     5101 cttgctgctg ccaataccgc ccaggcggca ggaaatggtg cagctcgcga tcgctggcta
     5161 caacaggcag aacaacgtgg actgggaaac aatgccctct actggtggct gcatgcgcaa
     5221 cgttacattc ctggtcagcc ggaactcgca ctgagcgatc tcacgcgctc aatcaatatt
     5281 gccccttctg ccaacgctta cgttgcgcgg gcgacaattt atcgccaacg tcataatgtc
     5341 ccggcggcgg tgagtgattt gcgcgccgcg ctggaactgg aaccgaataa tagcaacatc
     5401 caggcagcgc ttggttacgc cttgtgggat agcggtgata tcgcacagtc gcgggaaatg
     5461 ctcgaacagg cgcataaagg gcttccggac gatccggcac tgatccgaca actggcctac
     5521 gtgaaccagc gtctggatga catgcctgcg acgcagcact acgcccggct ggtgattgat
     5581 gacattgata atcaggcgct gataacccca ctgaccccag agcaaaatca gcaacgcttc
     5641 aatttccgcc gtttgcatga ggaggtcggt cgccgctgga cgttcagttt cgattcttct
     5701 atcggcttgc gttccggggc aatgagcacc gctaacaata atgtcggcgg cgcagcgcca
     5761 gggaaaagct atcgtagcta cgggcaactg gaagccgagt accgcatcgg acgcaatatg
     5821 ctgctggaag gcgacctgct ctcagtttat agccgcgtct ttgccgatac cggagaaaac
     5881 ggggtgatga tgccggtgaa aaatccgatg tccggcaccg gtctgcgctg gaagccgctg
     5941 cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg ccaaaatggc
     6001 gcatccgata ccatgctgcg cgccagcgcc tcattcttta atggcggcaa atacagcgac
     6061 gaatggcacc cgaacggttc aggctggttt gcccaaaacc tgtacctcga tgcggcgcaa
     6121 tatatccgcc aggatattca ggcgtggacg gcagattatc gcgtcagctg gcatcagaag
     6181 gtagctaacg gacagactat tgagccttac gctcacgttc aggacaacgg ctatcgtgat
     6241 aaaggcactc agggcgcgca gcttggcgga gtcggggtcc gctggaatat ctggaccggc
     6301 gagacgcact acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta tcaacatacc
     6361 tttaaggcga ttaatcaacg taacggagag cgcaacaacg cgtttctcac cattggagtg
     6421 cactggtaaa tgcgtaagtt cattttcgta ttgctgacac tgcttttggt cagccctttt
     6481 tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc gagatagtca ggttaccgat
     6541 acccagtggc aggggctgat gagtcagtta cgtttgcaag gcttcgatac cctggttttg
     6601 caatggaccc gttacggcga tgcattcacc cagccagaac agcgcgcgtt attgtttaag
     6661 cgtgccgcag ctgcgcaaca ggctggcctg aagctgattg tcgggctgaa cgccgatccg
     6721 gaatttttta tgcaccagaa acagtcgtcc gcagcgctgg aaagctatct taatcgcctg
     6781 ctggctgccg atctccagca agccagatta tggagcgccg cgcctggcgt aacgccggat
     6841 ggctggtaca tcagcgcgga aattgacgac ctgaactggc gcagcgaagc tgcccgtcag
     6901 cctttgctaa catggttaaa caacgcgcag cggctgatta gcgatgtttc agcaaaaccg
     6961 gtttatatca gtagtttttt cgccggaaac atgtcgcccg atggctatcg ccaactgctg
     7021 gaacaagtta aagcaaccgg cgttaatgtc tgggtacagg atggcagcgg cgtggataaa
     7081 cttaccgctg aacagcgtga acgttatcta caggccagcg ccgattgcca aagtcccgcc
     7141 cctgccagcg gcattgttta tgaactgttt gtcgccggca aaggcaaaac ctttacagcg
     7201 aaaccgaaac cggatgcaga gatcgcctcg ctgttagcga aacgttcctc ttgcggtaaa
     7261 gacactctct atttctctct gcgctatttg cccgtcgcgc acggcattct cgagtattaa
     7321 atctcctcca ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg tcagaattcc
     7381 atcaagatta attgctaaaa agcgtgctaa agaaagaaga tcgttatcgg cgaacgggcc
     7441 gatcactcat gcaggaagca tagatccttt gagcggagtt tctgatatct gaggagtgcg
     7501 aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa gaggtagagg
     7561 taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa ctacctggcc gccttcgcgt
     7621 gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt attaatcccc cacatcaacc
     7681 gtaacatcat caacgattat ctgttgtttt taaataagaa cttaacctgt gtaaagccct
     7741 ggtcgcggct ggcaaccccg gttatcgctt gtcatagccg tacaccggaa gtgttccggc
     7801 tagccgccaa ccacagcaag cagcaaccca gcaaaccctg cgaagcggag ttgacgcgcg
     7861 cattactttt taccgtattg tctaactttc ttgagcaatc gcggtttata gccctactga
     7921 tgtatatctt acgcagcagc gtccgcgaca gcgtctgccg cattattcaa agcgatattc
     7981 agcattactg gaatctgcga attgtcgcca gttcgctatg tttaagcccc agcctactca
     8041 aaaagaaatt aaaaaacaaa aataccagct atagccagat tgtcacagag tgtcgtatgc
     8101 gttacgccgt acagatgtta ttgatggata acaaaaatat cactcaggtg gcgcaattat
     8161 gtggctatag cagcacgtcg tactttatct ctgtttttaa ggcgttttac ggcctgacac
     8221 cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg gtgaagggca aagcggaaac
     8281 ggataagacg ggcataaatg aagaagaaat ggcgcgccct gcaggattcg aacctgcggc
     8341 ccacgactta gaagttccta gaacgacatt ttaagtcaac aacttaccgc gccatctctg
     8401 cgctcacacg tcccactacc tcaaaacatg taaagccttg caagccattg tgaggcctta
     8461 tgtgtctcag ttttgtccct cttttttgta ccaaaaaaca tagcaattga ggataaacct
     8521 catgctattt tcgcttatat gcctctaaag gcatggcgct taaatagata aaaacatcac
     8581 aaaagcataa aaaaccacac agtaaaaccg caacataaaa caataacaga taattaaacc
     8641 aaaaacagat agcgcattgt gataatcatt caatactaaa caaaatataa acagtggagc
     8701 aatatgtaat tgactcatta agttagatat aaaaaataca tattcaatca ttaaaacgat
     8761 tgaatggaga acttttatgc gggcgaaact tctgggaata gtcctgacaa cccctattgc
     8821 gatcagctct tttgcttcta ccgagacttt atcgtttact cctgacaaca taaatgcgga
     8881 cattagtctt ggaactctga gcggaaaaac aaaagagcgt gtttatctag ccgaagaagg
     8941 aggccgaaaa gtcagtcaac tcgactggaa attcaataac gctgcaatta ttaaaggtgc
     9001 aattaattgg gatttgatgc cccagatatc tatcggggct gctggctgga caactctcgg
     9061 cagccgaggt ggcaatatgg tcgatcagga ctggatggat tccagtaacc ccggaacctg
     9121 gacggatgaa agtagacacc ctgatacaca actcaattat gccaacgaat ttgatctgaa
     9181 tatcaaaggc tggctcctca acgaacccaa ttaccgcctg ggactcatgg ccggatatca
     9241 ggaaagccgt tatagcttta cagccagagg tggttcctat atctacagtt ctgaggaggg
     9301 attcagagat gatatcggct ccttcccgaa tggagaaaga gcaatcggct acaaacaacg
     9361 ttttaaaatg ccctacattg gcttgactgg aagttatcgt tatgaagatt ttgagctagg
     9421 tggcacattt aaatacagcg gctgggtgga agcatctgat aacgatgagc actatgaccc
     9481 aggaaaaaga atcacttatc gcagtaaagt caaagaccaa aattactatt ctgttgcagt
     9541 caatgcaggt tattacgtaa cacctaacgc aaaagtttat gttgaaggcg catggaatcg
     9601 ggttacgaat aaaaaaggta atacttcact ttatgatcac aatgataaca cttcagacta
     9661 cagcaaaaat ggtgcaggca tagaaaacta taacttcatc actactgctg gtcttaagta
     9721 caccttttaa cgaagttaac cagattttct ccccggtggt attgtataac cccggggaat
     9781 ttgtttacag ttacaagtag acaatatcca ctaattttgt aagcatttaa cctatgtcat
     9841 aacgtgtaat ctcttaccgc aacacaagac ctcaatatat tcaaatttta tctttattcc
     9901 gatattcaga gacacaatgc gaaaaataat tactcatttc aaagttgttt taacgttact
     9961 tctaccagta accgtatctg cccagcagat acagtggcaa tcctgtatgg ccagtcaatt
    10021 caaccactgg tttggtgagg aaaaaccgtc tcctgactta ctatgtggtt atttgtctgt
    10081 tccattaaaa tatacagaca caggcggaga tgcttcttat gaaaaaaaat cacaagtcaa
    10141 actagcgttg acaaaattgc cggcaaaaag caagcataaa ggaagtatcc tgataataag
    10201 tggtggtccc gggttaccag gcataaatcc ttatattaac tttgactggc cagtcacaaa
    10261 tcttcgtgag tcatgggata ttattggatt tgatcctcga ggcgtcggac agtccactcc
    10321 gacaataaac tgccggcaat cagatacaga gactcaggaa aacataaccg aaaagcaaca
    10381 agtattaaat aaaattaatg cctgtatcca taataccgga gccgaagtca ttcgccatat
    10441 cggctctaac gaggctgtat acgatattga tcgtattagg caagccttgg gggataaaca
    10501 actgacagcc gtggcgtatt cgtatggaac tcaaattgca gccttatatg cagaacgttt
    10561 tccctacaac gtaagatcta tcgttcttga tggagtcgtc gatatcgatg acctggagga
    10621 caacttcaca tggcaactca aacaggcaca gagttatcag gaaacgtttg atcgctttgc
    10681 atcctggtgt gcgcgtacaa aaagttgccc gctttcttca gacagagata aggcaataac
    10741 tcacttccat gagctattat caaaattaca tcacaaacct ttattagaca gtaagggaga
    10801 aaatatatct tcaggtgaac tcatatcatt aacaacagac cttctgctat ggcgttcatc
    10861 atggccaacc cttgcaactg ccatacgcca gttctctcag gggattgtca gtaatgaaat
    10921 tgaaactgcg ctcagtgctc cgatagcctc agaagagtca agcgatgctt cgggggtaat
    10981 cctctgtgta gatcaggggg atgagcaatt aacaccagaa gagcgaaaat cccgaaaaga
    11041 cgctcttgcg aatgccttcc cggctattaa ctttgacaat ggacgttccg attcacctga
    11101 tttttgtgaa ttatggccaa tacatagcga cctgaacaaa actcgcctga aaaatactgt
    11161 tctgccctct ggtttactgt ttgtagcaca caaatacgac ccaacaacgc cctggattaa
    11221 tgcccgtaag atggcagaga aattttccag cccgttacta acaataaatg gtgatgggca
    11281 tacattagct ctcaccggag ttaatttatg tgtagataaa gcagttgtac atcacctgat
    11341 cactccacaa aaaatagaaa atatatactg cccaggaaa
//
LOCUS       DAZFVO020000152        11239 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFVO020000152 DAZFVO020000000
VERSION     DAZFVO020000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.153"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1472)
                     /locus_tag="ACHKNB_RS19845"
                     /old_locus_tag="pgaptmp_003969"
     CDS             complement(<1..1472)
                     /locus_tag="ACHKNB_RS19845"
                     /old_locus_tag="pgaptmp_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4197385.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQAAAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAISLPMVGVTSLAVATGALAYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQE
                     MLIKAEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQA"
     gene            complement(1462..1866)
                     /locus_tag="ACHKNB_RS19850"
                     /old_locus_tag="pgaptmp_003970"
     CDS             complement(1462..1866)
                     /locus_tag="ACHKNB_RS19850"
                     /old_locus_tag="pgaptmp_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4197386.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VTVSDDMRSSTEDEAMLMMVSEGIAGGVRYGG"
     gene            complement(1893..2324)
                     /locus_tag="ACHKNB_RS19855"
                     /old_locus_tag="pgaptmp_003971"
     CDS             complement(1893..2324)
                     /locus_tag="ACHKNB_RS19855"
                     /old_locus_tag="pgaptmp_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4197387.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(2344..3093)
                     /locus_tag="ACHKNB_RS19860"
                     /old_locus_tag="pgaptmp_003972"
     CDS             complement(2344..3093)
                     /locus_tag="ACHKNB_RS19860"
                     /old_locus_tag="pgaptmp_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4197388.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(3101..3499)
                     /gene="gpU"
                     /locus_tag="ACHKNB_RS19865"
                     /old_locus_tag="pgaptmp_003973"
     CDS             complement(3101..3499)
                     /gene="gpU"
                     /locus_tag="ACHKNB_RS19865"
                     /old_locus_tag="pgaptmp_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HGU4197389.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(3512..4135)
                     /locus_tag="ACHKNB_RS19870"
                     /old_locus_tag="pgaptmp_003974"
     CDS             complement(3512..4135)
                     /locus_tag="ACHKNB_RS19870"
                     /old_locus_tag="pgaptmp_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4197390.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVLLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(4138..4419)
                     /locus_tag="ACHKNB_RS19875"
                     /old_locus_tag="pgaptmp_003975"
     CDS             complement(4138..4419)
                     /locus_tag="ACHKNB_RS19875"
                     /old_locus_tag="pgaptmp_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HGU4197391.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4412..4738)
                     /locus_tag="ACHKNB_RS19880"
                     /old_locus_tag="pgaptmp_003976"
     CDS             complement(4412..4738)
                     /locus_tag="ACHKNB_RS19880"
                     /old_locus_tag="pgaptmp_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HGU4197392.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSAAV
                     AVKINV"
     gene            complement(4826..6850)
                     /locus_tag="ACHKNB_RS19885"
                     /old_locus_tag="pgaptmp_003977"
     CDS             complement(4826..6850)
                     /locus_tag="ACHKNB_RS19885"
                     /old_locus_tag="pgaptmp_003977"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4197393.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQTGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQALQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(6795..8297)
                     /locus_tag="ACHKNB_RS19890"
                     /old_locus_tag="pgaptmp_003978"
     CDS             complement(6795..8297)
                     /locus_tag="ACHKNB_RS19890"
                     /old_locus_tag="pgaptmp_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4197394.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(8297..8509)
                     /locus_tag="ACHKNB_RS19895"
                     /old_locus_tag="pgaptmp_003979"
     CDS             complement(8297..8509)
                     /locus_tag="ACHKNB_RS19895"
                     /old_locus_tag="pgaptmp_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197395.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(8506..10629)
                     /locus_tag="ACHKNB_RS19900"
                     /old_locus_tag="pgaptmp_003980"
     CDS             complement(8506..10629)
                     /locus_tag="ACHKNB_RS19900"
                     /old_locus_tag="pgaptmp_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4197396.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(10626..11102)
                     /locus_tag="ACHKNB_RS19905"
                     /old_locus_tag="pgaptmp_003981"
     CDS             complement(10626..11102)
                     /locus_tag="ACHKNB_RS19905"
                     /old_locus_tag="pgaptmp_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HGU4197397.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVSVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2394 a         3544 c         2701 g         2600 t
ORIGIN      
        1 gcctgaagcg tcagcagggc agcatgagca ctgtcttcct gacgatcgcc cgcagacacc
       61 ttcacgccgg actgtttcgg ctttttcagc gtcgcttcat aatccttttt cgccgccgcc
      121 atcagcgtgt tgtaatccgc ctgcaggatt ttcccgtctt tcagtgcctt gttcagttct
      181 tcctgacggg cggtatattt ctccagcggc gtctgcagcc gttcgtaagc cttctgcgcc
      241 tcttcggtat atttcagccg tgacgcttcg gtatcgctct gctgctgcgc atttttgtcc
      301 tgttgagtct gctgctcagc cttctttcgg gcggcttcaa gcgcaagacg ggccttttca
      361 cgatcatccc agtaacgcgc ccgcgcttca tcgttaacaa aataatcatc cttgcgcaga
      421 ttccagatgt cgtctgcttt cttaaacgca gcctctgcct taatcagcat ctcctgcgcg
      481 gtatcaggac gaccaatatc cagcaccgca tcccacatgg atttgaatgc ccgcgcagtc
      541 ctgtctgccc aggtctccag cgtgcccatg ttctctttca ggcggcgggt ctggtcatca
      601 aaccctttcg tggcggcctc gttcgccgcc tgcaatgccc cggcttcatc gccggaacgc
      661 tgcaactgag caacatacgc aatctgctct gccgtcacgt tacggaactg gcgtgccatc
      721 gcaatcagcc ccgacgtcgg gtcggtggtc agttttccga aagcctctgc aaccttgtcc
      781 acctccacac cggaggcaga cgcaaaacgc gccacactct ggttaatggc atcaaactgt
      841 tcaccaccac gcacaccggc actgaccagg gctgccagtg actctcccgc ctggttaaac
      901 gtcagccctg ccgcctgccc ggctctggag agcgtcagca tgcgctctgc cgtcagtcct
      961 gactgattac cggaaagaac cagggtttta ttaaacgctg aaagcgtgga atccccctgg
     1021 taccaggcgt acgccagcgc accggtcgcc accgccagcg aggtgacccc gaccatcggc
     1081 aggctgatcg caccggcaag cccccggaac atggggatca tcccgccgaa tgagtccttc
     1141 acctgaccgc cctgttgcag caggatgagc cagggattct gaccaccggc aagctgcgtg
     1201 gccacgtcgg tgaactgcat gggtagcata cgcatggccg ctttatactg cccgacggac
     1261 atcccggctt tttgtgcagc cagggcctga cggctcagcc cctgttcaac ggcagcggcc
     1321 tgtttcttaa aagactggct gacacgcccg gccatcaaat ccgcaaggtc actggtttca
     1381 cccagttctt tctttacccg tgcagcctct tcagaaaaac gggttgaatc cagtgtaaga
     1441 acagctgtca gatcggcaaa attacccgcc atagcgtaca cctcctgcaa ttccctcaga
     1501 caccatcatc agcatggctt catcctctgt gctgctccgc atgtcatcac tgaccgtaac
     1561 gatttccttc ccgtcagccc caaagcggac accaccagaa agtcctgccg ctttccgcat
     1621 cagcatatcg tcctcatccg gcctctccgt ctgcgcttcc tcacgccggg ggacaagcag
     1681 actgaaatcc gagggatgca tatccggatc gcaaaaaaac aggctgagta cggcgtacgt
     1741 cagcccggaa aaatgcatat ccagctgagt atcgtgaaaa taatgcgtgc ggtaaaaacg
     1801 gtgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct
     1861 ccccatctca cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga ctttcccgca
     1921 gaaaaatcat catcagtcag cgcgttattt ttcgccactt cagtaatatc agtatccgga
     1981 cgaacagctt cgatcatccc ggacaggcac aacacaacgt cttccgcccg ggcaatggca
     2041 tcggcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc attcattgac
     2101 ggtgacgccg ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgccccg
     2161 gttctgacaa ggtcctccac gctcacctgc aggttaccgc tggcttcagc ctcttctgcc
     2221 cgccgtttca ggagggcaag atgctcaata cgctgcagcg cagacagctc agaaagcgtg
     2281 acagacacac cgttatattc aaattgttct gttttcagaa acatgcttta tcactccctc
     2341 agcttaaccc gctgcaccat ccgtgacggt aatttccgcc accgccgcaa actcaccatt
     2401 gccggtgacc acagggatct gtgctttacc tgccgcaaca cctttcaccg tgatcgtgtt
     2461 ccccttcacg gtaatggtgg cgaaattctg attcgccgac gtggcacgga aggttttatc
     2521 cgtcgcgcct tccggctgaa cggccacggt cagggtgata ttctgacctt ttgccacatt
     2581 gcccgttggt ggcgtcacgg taataccggt gaccggtgtg atgtccccct gatcttccgc
     2641 cagcgacgga cgaccgatat tggtgatttt taccgtacgg gtgatcacct ctttggcggt
     2701 caccgcttta ccaatggcgc tcacccagcc acggaacaca tccaccgtac cgttcgggaa
     2761 gcgaattttg taggcccggg tctcactgct gtcaaaccag gcaatcaaat cgcgctgccc
     2821 tttctcaccc ggcttccagg ccagcgtaaa actggtgtca ccggcagatt tctgcccctg
     2881 cccggtggat acccagtcag catcctcatc atccagatag ttatcatcgt aggattccgc
     2941 cgtcatctcg ccgggggtca gatccttcac cttcgccagt cgctgccagt catcgtctga
     3001 caacgggttt gcataagcat caccagtgcc ggtgtacacc cacagtgtgg taccggaacc
     3061 tttcaccggc tcaagaggat ttggtgttgc catatcgtcc tcacatctcg taggtaattt
     3121 tccacaggag atctgccgat ccccacatca taaactcatc atcccggcgg tagtcatacc
     3181 cctgaagatt catcttcagc agtaatgcac tgagcccggg aactgcctcc agcgcaggaa
     3241 gaattttttc ttccatccac atatccagtg ccgagtccgg ttcttttgcc ctgagaaaaa
     3301 cctcaatatg cagtgtcgcc tcccaggttc cctcatcaac gaactcgtca gcagcagacg
     3361 catctgtcag gtaaacagca acagcaggca gttcctgttc atcaataaaa accgggcgac
     3421 cgtcaaacca gctcaccctc tcagaaatat tttctttcag ggcagacaga actgccgccc
     3481 gtatttcacg gtgtttcata caccctccca gtcattttct tttcagcacc aggcgtaact
     3541 gatgcgtcat ggctttcatc atctgcgccg gtaatttttc ccggtacatc cggtcccgtt
     3601 cacgttcaaa ggtttctgcc agcggtccgg cagtcggaat cttcaccact tcgatcggca
     3661 gacggtggcg tttcggcctc cctttgctgt cagcgccggt ggacgatgat gcccacggca
     3721 tacgctgcat cacatgccag cgtccgttag ccagccgggt gataaaggca tccgggatcc
     3781 gtcttttccc cacaatcagc acgctgccac cgcctttcgt gaccgaacgc tcgcctttct
     3841 ttcgtcgctt tctgcgggaa agcagcacgc tggccgtccc cagttttatg gcgggcagat
     3901 taccggtgtt gatgatgacc ttcgcataaa ccctgtctgc gctggcccgt tgcaggcgga
     3961 tacgctcacg gatgagacgg cgcggaaccg ccagctccct ggcaaccgaa gaggccgttt
     4021 tcgcgatgat ggactccgcc acacggttaa gtgtcgtggc ggcagcccgg ggaacggcac
     4081 ggcggtcaat tgcatccaga tttttcatgg cctgcgccag accttttatt gccatactca
     4141 ttcctgttcg acaaaaatcc ggggtttacc gttgtacgtg tcataacggg tcaccgtcag
     4201 tgtgcgcccc gcaaacacaa caacatcatg acgggccgga cggtaccggg ctgaaaacac
     4261 caccagtgac aactggctgc ccgaaagcgc ccccatctcc gcggactctt cctccggcat
     4321 cacgtcgtac acgacgccgt taatctccgc ctgtttgccc atcatccgaa cggtcgccac
     4381 gtccatccgg caacacattc gcgtaaacag atcagacatt gatttttacc gccacagcgg
     4441 cgctgtttgc aggagcattt tcccaggcta ctcccgctgc caccgcaccg tctgcagcca
     4501 gctgcacaac cccgtccttc agataaacca ccgcgccgga ctgaatgtcg tcagcagact
     4561 gtttgggcag caggaacacg ccttcggcaa aaccgtcacc ggcctcaccg gcaggaatat
     4621 cggtaatggc cacggccacc atactgccga ccaccaccgc agcaccgctc aggatggtct
     4681 gatctccggc attcaccagt tcaatggtgg taccgtcctg tacaaaattt ttcgccataa
     4741 tgctgtttct ccggacagcc cctgtggggc tgtttttcag gcataaaaaa agccctttcg
     4801 ggcagtgatt gtgataacgc ggttatcagg ccaccgacga acgcaccagc ccgcgccagt
     4861 caagtggtgc cactccggca tcaatacgga tttttgtggc aataccgtca gtggtgaaac
     4921 cttcctgctg atcaatgtat ggcgtgtcca caccatccag ccaggccact tcaatggtgt
     4981 cagtgccctg tgccgccgcc agataccagg ttttcggatc tgccgcatca agacgcgctt
     5041 ctgcaatcac ctcagcaaag ttctggatgg ggttaatgac accggcgttt gcatccgccc
     5101 ctttcacact ggccgatttg atggtctggt tcgccaccgt ctccagtgcc accggtacca
     5161 gcataaaggc cggacggata ttcagggcgc gatcgccttc tttctgcagg cgcatcatct
     5221 gacgggccgc atccagtccg gaaacggaga tccccccggt ggcaatattt ttgtgatcgg
     5281 catggaacag cgccttaccg tcagacagtt tcgggttatc cgtcagcacc ttgtaaacca
     5341 ggtcaccaat cgttgccttc gccgcacgcc ccatcttcat cggcacgtcc accagcatat
     5401 tcagatcatc attgataatg gcctggcggg tgatggagaa aatctccccg taggtggcca
     5461 gagcaatggt ctccttgcga tctgaggtgg tgatgtattt atactccgcc ccctcacgaa
     5521 cctggcgcag agaaccaaaa ccgcccatcc ccacgcgata cgctgttttg aagtctgaca
     5581 ggcgtccctt acgggtccac ttctggaagg tttcttctga ttcctcccag ccctggatca
     5641 gccccttgtt cgacacatcc agcagaatat tgccaaaatc agaggtgctg tgcgtcagcg
     5701 ccagcccgac catctgcatg gggttataac tggccacccc aataccgcgc tccgtcagtg
     5761 acatacgagc ccattcacgc agggtcatcc cgttataggc gttatccttc tcgacatttt
     5821 caaatccggc acgggccagc atcgcctggc ggatcccgtc tcccacaaaa ttgccgtttc
     5881 cggcataaat atgggccggt gtgtttttgt tggtcggcga tgactccttg cccatttcat
     5941 tcagaaggcg ctcgcgggcc atttccagcg aacagtcagg atcggccaca cactgcgcct
     6001 gaagcgcctg atagcgaccg ccaaacatgg caaacagatc gttaatgcct gacatgcggg
     6061 ctttctgctc agccataacg cgggcgcgaa tggtcgcctc atcagacact gccggtaccg
     6121 gtgatggttc tgttaccgcc ggtgcaggga ttgtcactgt ggtatcacgc ggggcactgt
     6181 tgcgtggcgg agtaatcatg tttcggatgg attccggcat ctttttaaat tcctctgtac
     6241 gttttgactg aatacatgcc attgcctcaa cagcgggtgt cacctggtca gcaaatccgt
     6301 gtgccagaca ttcggcaccg gacatccagg tttcatccgc cagcatggcg gcaatttcat
     6361 cggtggtttt tccggttttc tgcgcatagg ctggcaacag taccgattcg actttatcca
     6421 gcaaatcggc ataactgcgc atatcctcag catccccgcc actgaatccc catggcttat
     6481 ggatcatcat gaaggcattt tccggcataa tgaccgtatc accggccatc gcaatcacag
     6541 atgccatcga ggcggcaacg ccatccacat acacggtaat ggtcgccccc tgatttttca
     6601 gggcattaaa aatggcgatg ccttcaaaga catcgccacc cggtgaattg atatggagat
     6661 taatgtgggt gatatcaccc agtgcattca gttcgctgac aaactgcttc gcggtaactc
     6721 cccagaaacc aatctcgtca taaatataaa tatccgcgtc acccggcccc ccagtctgca
     6781 tcctgaacca ggatttattc ttcatgctgg ctttcggtgt cgcgctgata ctgtcgttca
     6841 gttccggcac tgttgcctcc tttgtcgttg acggggtcag tatcaaagac cagccccagt
     6901 ctgctgtttt catcaatttc agccttgcgg cgacgtttga cctcatccgg attgcgcccg
     6961 ccggcacgca cccagtcaga ttctgtcgct gcaccacccc ggatctgaat tctccaagct
     7021 tcagcttcct taaccgggtc gatccacggc atcaccggac cggaatacgt cgcgttatat
     7081 agcgttttca tctccacatc cgccggaatt ttcagcagac ctgccgcaac caccatattc
     7141 agccatgtcc ggtacaccgg gcgggttacc gcgccaataa aacagtcctg caggatcagg
     7201 taaccatccg tggactcgac cagctcctgc cgctgggcgc tgtaggtgcc gttatagtta
     7261 cgcgccgcac tggaaaaact cagacgactg cctgctgcca ctgcacgcaa ctggccgttg
     7321 cggaaagttt caagattggg attgggacgg tcagatttga ccatgccgat atcctcgccc
     7381 ttgcgcaaat cgtcataaat aatacccggg gtgatatgga cttcccgctc ggtttctttg
     7441 atccccggat cttcatagtc ctgtccgtca cctttacgga tatacagtcc cagcgccgca
     7501 gcaatacgcg ccgctgtcag ttccgcatcc tcatactcct taagggcact gatccgcatc
     7561 agcacccccg ataacatgga tgagcctcgc gtctgatgca gacgacgagt gaacttcagg
     7621 tggatcattt ttccggcagc gatttctttc gtatcactct gccgaccgct gaccggataa
     7681 tttttataaa ccagatattt tttcggtctt ccccactcat caagaaaaac cccctgattc
     7741 agtccggcgg attcatctgt gcgcatggga acaaaatccg gctccatcgc ctcaagccag
     7801 aatggcactc ccgccgtccg ttccagaccg tttcccgcac cactgaccat ctgcgcaaac
     7861 acttcaccat cccgcagcca ggtccgcagc agtaaacgtt caagcacagg acgggtatac
     7921 tgccctgtca catccggact cacggaccat tcagcccaca aacggcggat atccgcagcc
     7981 agctcagccg ccatttcccc gttttttcgt aatggctgag gctccacaat aattcccctg
     8041 gcaccaatca cccgctcttc cagcttgtca aacacaccaa tcaccaggtc atgattgata
     8101 tcaagaaaac gggcctgctc ccgcagggaa accgcaccgt atttactgag ctgatcggca
     8161 gagcgatttt cccgccgggc tttatgtgtc cgggtcggtt tcaccgcctc ataggccatg
     8221 attaacgccc ttgaacgcag tctggctgct ttccacccgg gggaaaacac gcctatcaca
     8281 tcatcaataa ttgccattaa aacctcgcca gtttaaatcc tggttttccc cgcctgcggc
     8341 tcaccatcgc ggcaagcctg cgttcccact cctgacgtcc ggcgcggatc tgagaaaggc
     8401 tttccagcgt cagttgctgc ccgttgaagg tgacagactt cccctccagt acggccattt
     8461 ccgcttcacg gtaccgctgt atcatttctc tggcttcttc tgtgctcaca accagcctcc
     8521 tgatgttatc catggattat cttccgcacg ctccgtacgc agttttttct tccggcgacg
     8581 gcgtttttct gccccggccg tcagttccgg ggataccgtt tcaccagaac gctcctgcgg
     8641 gaagacgagc cacgtttccc gctgtgccca gtccggtgcg gagggccagc ggatcttttc
     8701 gtaaccatgc agaacggcaa gcgcatccgc ataaaccagc aggtcaaacg cctcgttagc
     8761 gcccctgccc ggttttcgcc attttccgtc actgccgcgc tcttcatagg tcagctcatc
     8821 gtaaaaccac cgccccagcc agtcgggaaa gtggatatag ttcggccctg gtgtgtcacg
     8881 ccacagggca ttatttacac ggtccttaaa cgcatccgtc tgaaccagcc acagcgcgac
     8941 atcgccactg gctctggcac ggcgggcact tctgccggta ttatccggga aggtacggtt
     9001 aatcagcctg tcacggcgaa gaccatcccc cttgaacaga aacaccctgt tgcccagtcc
     9061 gtcactccgg caacgacgcc agaaacgata ggcgttatct gtcaccccgg cttcccctcc
     9121 cgtatccacc gccatggcca tcagacgcat gcgcacatcc ggatcagaag ccagcggcca
     9181 tgttttatgg aacacatccg tcagcaacaa atcccagtcc tccggatatg ccgccggatc
     9241 aaccggcaga ctttcaccgt tgggactgca gcgcagtgaa tgccggatgt tgtagcgatc
     9301 aacaatccag cgttccccct gctctccgta tccggtgatc tgcacaacaa aacggcgatt
     9361 tttaccgccc tgtacgtcaa ccgttgcctc aataaaacgc acaccatccg gcacagatcg
     9421 ccggggaaac ggctcggcac gctgttcaag cagttcactt ttacgctgtt ccgtggctga
     9481 acggggcaga tagggtcgtc cgatatcggt gttcaccacc gctttcaggg tctcttcact
     9541 gccggttcgc tcatactctt cttctgccgc cagcagttta aaaatcagtt gttcccaggt
     9601 ctgaaacgcc gcagccggcc cctccatcca aaatgacgca atccgggaat ttcgtggcgt
     9661 tccggtgata ctgccgtccg ccgccgcccg ttcaccttca cgaagccaga tcccctggtt
     9721 attcagttca cgcttctgtt ccggagcaat caggccgcgg cagtgcggac acatcaggcg
     9781 ggccgcctgc ccggcagcca caaaatcagg gttattccgg taaccggtca tgttatccat
     9841 caccggctga aaatattccc cgcagtgcgg acacggccag taccaccggc ggcggtctcc
     9901 ccggttatac agtgacagga tccccgttgt tggcggtgcc tcatgtgcgc cgccacagcg
     9961 ccatttggtg tcagtgatat cccgcccggg cgaactttcg accagggtca tccccgagga
    10021 cataaaggtg gtggtacgct ttgaggccag ggtgaaggca tccccttcac cgtcgacgtt
    10081 ctcaggaaaa cggtcataat ccgtcagcgc cacacgacgg taatccgaag aggaaaatac
    10141 agtgatcgac ggccagccaa ttttcaggaa tgagccgtca agaaacattt tgtcgtggac
    10201 gttgttgtca ttacgggaag ggctgaggcg cttactgacc tccggactgt ggcgaaacgt
    10261 cctggaaaga cgcgttctgg aatgctcacg cgcctttgtc tcggtcatct gcaccaccag
    10321 catatccgcc ggatcacaga tgatgccgta cacaatccag ccatcaatca gcccttcggt
    10381 tttcccggtt cgcgcaggtc ccacaaacac caccgcgtca tattcacggg ctgataatgt
    10441 attaatgggg tcaatcatat agggcgtcag cgatgactcc cacggaccgg aagtattggc
    10501 tccccgtggt acccgcatat aacgcctgat ggcttccgct actggtaacc ggccaggtgg
    10561 gcgaaacagc gaggccactt cgcgccagat atcggatgcg cggctatggc tctcgttcac
    10621 ctgattcaca tatcggcctc atcacaacag tcaatgactg ccttttccag tgtgtcgcgg
    10681 atctcatcaa ccacaatctg tacttcattc agttgtgatg cggtccaccc cctgtccctc
    10741 tccagccggt caggccaggt ttccagtacc tgaactatcg ctttcaccac gacagaaaac
    10801 gaccgcctga catcactgac tgacacgagc agaccggttt cctgctcaaa tttcagtcgg
    10861 tcacgttctg actggtacca tgctttacgc gcatgaggat ccatttcctc gttatctaca
    10921 ggcagaggag ctttcatcag ctcggcaagg atatctgtca gtcggtacag tttgagatta
    10981 ctctcatgac caccggctgg gcttatgttt tttacccgag ccgcgacagt ctgtcgatga
    11041 gcaccggata atgcagccag ttgggaaata ttcagatgca gattttttaa ttcacgatcc
    11101 ataacttccc ctgaaaatta tgtaaataca cgccagtgat gaacaaaaaa caatcaactt
    11161 cgacactaaa aatttttatt atggtatata tcaatgattt acactggtgg tgatggtgcc
    11221 ataaaaatca aaaaatgcg
//
LOCUS       DAZFVO020000153        11164 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFVO020000153 DAZFVO020000000
VERSION     DAZFVO020000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.154"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            46..1617
                     /gene="garD"
                     /locus_tag="ACHKNB_RS19910"
                     /old_locus_tag="pgaptmp_003982"
     CDS             46..1617
                     /gene="garD"
                     /locus_tag="ACHKNB_RS19910"
                     /old_locus_tag="pgaptmp_003982"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HGU4197398.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1766..2101
                     /gene="prlF"
                     /locus_tag="ACHKNB_RS19915"
                     /old_locus_tag="pgaptmp_003983"
     CDS             1766..2101
                     /gene="prlF"
                     /locus_tag="ACHKNB_RS19915"
                     /old_locus_tag="pgaptmp_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HGU4197399.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2101..2565
                     /gene="yhaV"
                     /locus_tag="ACHKNB_RS19920"
                     /old_locus_tag="pgaptmp_003984"
     CDS             2101..2565
                     /gene="yhaV"
                     /locus_tag="ACHKNB_RS19920"
                     /old_locus_tag="pgaptmp_003984"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HGU4197400.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2620..3429)
                     /gene="agaR"
                     /locus_tag="ACHKNB_RS19925"
                     /old_locus_tag="pgaptmp_003985"
     CDS             complement(2620..3429)
                     /gene="agaR"
                     /locus_tag="ACHKNB_RS19925"
                     /old_locus_tag="pgaptmp_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HGU4197401.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3678..4958
                     /gene="kbaZ"
                     /locus_tag="ACHKNB_RS19930"
                     /old_locus_tag="pgaptmp_003986"
     CDS             3678..4958
                     /gene="kbaZ"
                     /locus_tag="ACHKNB_RS19930"
                     /old_locus_tag="pgaptmp_003986"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HGU4197402.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            4981..5454
                     /gene="agaV"
                     /locus_tag="ACHKNB_RS19935"
                     /old_locus_tag="pgaptmp_003987"
     CDS             4981..5454
                     /gene="agaV"
                     /locus_tag="ACHKNB_RS19935"
                     /old_locus_tag="pgaptmp_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HGU4197403.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5465..6244
                     /gene="agaW"
                     /locus_tag="ACHKNB_RS19940"
                     /old_locus_tag="pgaptmp_003988"
     CDS             5465..6244
                     /gene="agaW"
                     /locus_tag="ACHKNB_RS19940"
                     /old_locus_tag="pgaptmp_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HGU4197404.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6234..7112
                     /gene="agaE"
                     /locus_tag="ACHKNB_RS19945"
                     /old_locus_tag="pgaptmp_003989"
     CDS             6234..7112
                     /gene="agaE"
                     /locus_tag="ACHKNB_RS19945"
                     /old_locus_tag="pgaptmp_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HGU4197405.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7130..7564
                     /gene="agaF"
                     /locus_tag="ACHKNB_RS19950"
                     /old_locus_tag="pgaptmp_003990"
     CDS             7130..7564
                     /gene="agaF"
                     /locus_tag="ACHKNB_RS19950"
                     /old_locus_tag="pgaptmp_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HGU4197406.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7561..8694
                     /gene="nagA"
                     /locus_tag="ACHKNB_RS19955"
                     /old_locus_tag="pgaptmp_003991"
     CDS             7561..8694
                     /gene="nagA"
                     /locus_tag="ACHKNB_RS19955"
                     /old_locus_tag="pgaptmp_003991"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGU4197407.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYINTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9045..10199
                     /locus_tag="ACHKNB_RS19960"
                     /old_locus_tag="pgaptmp_003992"
     CDS             9045..10199
                     /locus_tag="ACHKNB_RS19960"
                     /old_locus_tag="pgaptmp_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HGU4197408.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10212..11072
                     /gene="kbaY"
                     /locus_tag="ACHKNB_RS19965"
                     /old_locus_tag="pgaptmp_003993"
     CDS             10212..11072
                     /gene="kbaY"
                     /locus_tag="ACHKNB_RS19965"
                     /old_locus_tag="pgaptmp_003993"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HGU4197409.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2673 a         2892 c         2965 g         2634 t
ORIGIN      
        1 accaggtcct cattttaata acccctggct ggagaatata gtacaatggc caacatcgaa
       61 atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga taatgtggca
      121 attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg actggaatta
      181 attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc taatggtgaa
      241 attattcgtt atggtgaagt gattggttac gccgtgcgtg caatcccacg cggaagctgg
      301 atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct gccactggca
      361 accaaagtcc cggccccctt accgccgctg gaaggataca cctttgaggg ctatcgcaat
      421 gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt ccactgtgtg
      481 gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc gaaatacccg
      541 aacgtcgatg gcgtggtggg gctgaatcat ttgtacggct gtggcgtggc gattaacgca
      601 ccggcggcag ttgtgcctat tcgtaccatc cacaatattt cgctgaaccc taactttggc
      661 ggcgaagtaa tggtgattgg tctgggttgt gaaaagttgc agcctgagcg cctgctggtc
      721 ggcacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag tttgcaggat
      781 gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc cgaacgccat
      841 ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact ggttgtcggc
      901 atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg caaacccggc ggttggctat
      961 gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tctcagaagt cacggaagtg
     1021 cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg caaacggctg
     1081 ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga ccgcagcgcc
     1141 aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa ggcactcggc
     1201 tccattgcta aatcgggtaa aagcgcaatt gttgaagtgc tgtcgcccgg tcaacgcccg
     1261 actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg tggcacgcaa
     1321 caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac gccgtacggc
     1381 ctgatggcgg tacccgtcat taaaatggcg acccgcaccg agctggcgaa ccgctggttt
     1441 gatttaatgg atattaacgc gggcactatc gccaccggcg aagaaaccat tgaagaggtg
     1501 ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa aaccttctcg
     1561 gatcaatggg ggctgcataa ccagctggca gtgtttaacc cggcaccggt gacctgattt
     1621 cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg tttaaagccc
     1681 ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt tgagcagaaa
     1741 acaggctgta aaaggacagt gaatcatgcc cgctaatgct cgctctcacg ctgtactgac
     1801 cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcgccag tgcgtgaggc
     1861 cttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg gtgggcaagt
     1921 ctttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat ttttgcgttt
     1981 tctggatgca gatatccaga acaacccgca aaaaactcgt ccattcaaca ttcaacaagg
     2041 aaagaaactt gtcgctggca tggacgtcaa cattgatgat gagattggcg acgacgaata
     2101 atggattttc cacaaagggt taatggttgg gcgctatatg ctcatccctg ttttcaggaa
     2161 acctacgacg ctttagttgc cgaagtcgag acattaaagg gaaaagatcc tgaaaattat
     2221 cagagaaaag ccgccacaaa gttattggcg gtagtccata aagtgattga ggagcatatc
     2281 acggtcaatc catcatcacc ggcattccgt catggcaagt cgttaggctc tgggaaaaat
     2341 aaagactggt cacgggtaaa atttggtgct ggtcgttatc gtctcttctt tcgttatagt
     2401 gaaaaagaga aagtcatcat tctgggatgg atgaacgatg aaaacactct gcgcacctac
     2461 ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt taaaaagagg acatcctcct
     2521 gccgactggg aaagcctcac ccaagagaca gaagaaaacc attgatggtg ttcacaatgc
     2581 ccgccgaacg tattcgtgtt gaccagcggg caccctttct cactccccga ccagaatcac
     2641 ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt catcaacaat
     2701 gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat tgaatttact
     2761 ggaatcggtg accacgatga tccgttccgc aacttcgcac atccgacggt ttaaacgggc
     2821 ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta caccaagaaa
     2881 cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt aaaaagattg
     2941 cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg cttccagcaa
     3001 cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtgct tacgcatcag
     3061 acgagcaatc tcaaaagtgg tagtcccgga atcgaggatc acccgatgac caggctgaat
     3121 caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagcg cgcttttatc
     3181 ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc caccataggc
     3241 acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg tcacagtaga
     3301 tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc gcagacgctg
     3361 aatgatctgt tctcgtcgct cgctggtgcc agtcactcgc ttctcacctg aagcgtcggt
     3421 attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc ctattaacgc
     3481 ctttctatta agcaaatgca agcccacctt gcccattgac gcaagctact ctcgtttcag
     3541 tgactttcat tatgtttctt ttgtgaatca gatcagaaaa ctattatctt tcgttttatt
     3601 tttatctcac catgacgcag tatcaactga aacaaaacga aagattaata tcgcagtaat
     3661 ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac aaagcgggca
     3721 aaacaaatgg aatttatgcc gtttgttccg cacatccgct ggtgctggag gctgcaatcc
     3781 gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat caggtagacc
     3841 agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt tgtcagctcg
     3901 ccgactcgtt gaatttcccg caggatgcgt tgattctggg tggtgaccat ctggggccaa
     3961 accgctggca aaacctgccg gccgctcagg caatggccaa tgccgatgat ttgattaaaa
     4021 gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc tgtcaggacg
     4081 atccgattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg gcgaaagtgg
     4141 cggaagaaac ctgtcttgaa cactttggcg aagccgatct ggagtatgtc attggtaccg
     4201 aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc accacgccgg
     4261 atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa ggtttgaatg
     4321 ccatctggcc acgcatcatt gccctggtgg ttcaacccgg cgtcgaattc gatcacacca
     4381 acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc gaaaactacg
     4441 aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatcg ctgcgccagc
     4501 tggtgattga ccactttgcc attctgaaag ttggcccagc gctgaccttc gccctgcgtg
     4561 aagctctgtt ctctctggcg gcgattgaag aagaactggt gccagcgaaa gcctgttctg
     4621 gtctgcgtca ggtgctggaa gacgtgatgc tcgaccgccc ggaatactgg caaagccact
     4681 accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta tagctactcg gatcgcgtgc
     4741 gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt cgtaatctgg
     4801 cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcaa tacgtgaaag
     4861 ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tatcaaccat attcaggaca
     4921 tcctggcgca gtactacaca gcctgtgaag gccaataagc aaaacaaaga ggaacacgct
     4981 atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca ggtcggcgtt
     5041 cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga ggttgccgaa
     5101 gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc cgtacgtttc
     5161 tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca gaaaatcctg
     5221 ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt tccggtgaat
     5281 cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc caaaacggtt
     5341 tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg ggtggaatgc
     5401 ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact ttgagggatt
     5461 catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct ttatcgctgg
     5521 cctggatatg tttaacggcc taacacatat gcaccgcccg gtggtcctcg gcccgttggt
     5581 cgggctggta cttggcgatc tgcataccgg aattttaacc ggcggtacgc tggaactggt
     5641 gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta tcggtactat
     5701 cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc
     5761 cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg tgatgtctgg
     5821 cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca ttgaacgcgt
     5881 gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt tcctgcctat
     5941 ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac gattaatcga
     6001 cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc tgctgaaaat
     6061 catgatgaaa aacgtctaca tcccctactt catcctgggc tttgttgccg cagcctggct
     6121 caagttaccg gtgctggcta ttgctgccgc tgcgctggcg atggcgctga tcgacctgct
     6181 gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg aagatggcat
     6241 ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga aacattgctg actggcgtca
     6301 atgaaaacgt gtatgaagat cagagcattg gcgcggagct gacgaaaaaa gatatcaacc
     6361 gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt atgcaggctt
     6421 ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat aaacgcgacc
     6481 tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt ctggtgacat
     6541 ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac agtattcaga
     6601 gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg tgatgcgatg ttctggctga
     6661 cgctactgcc gatttgtggc gggataggcg caagccttgc gctacaaggc tctattcttg
     6721 gcgcagtcgt atttattgtg ctgttcaacg tggtacacct ggggctgcgt tttggtctgg
     6781 cgcattatgc ttaccgcatg ggcgtggcgg cgattccact cattaaagcg aataccaaaa
     6841 aagtcggcca tgctgcgtct atcgttggga tgacggtaat cggcgcgctg gtggcaacct
     6901 atgttcgttt aagcaccacg ctggaaatca ctgcgggcga cgcagtggtt aagttacaga
     6961 ctgatgttat tgacaaactg atgcctgcct tcttaccgct ggtctacacc ttgaccatgt
     7021 tctggctggt acgccgcggc tggagtccgc tgcgtctgat tgcagttacc gtggttctcg
     7081 gcatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga tgttaagtat
     7141 tattttgaca gggcatggcg ggtttgccag cggcatggaa aaagcaatga agcaaattct
     7201 cggtgagcaa tcgcagttta tcgccatcga ttttccggaa acctcaagca ccgcgctgct
     7261 tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca ttgtttttct
     7321 gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga tgcaaaaacc
     7381 gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta ctggagatgg tgctggagcg
     7441 cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac accgagggct
     7501 gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg aggaaggaat
     7561 atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggct cgatgaccat
     7621 cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt gggcgtgact
     7681 gaacgcgatg cggaactgct ctgccccgct tacatcaaca cccatgtaca cggtggtgcg
     7741 ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat gcacaaggca
     7801 cgcgaaggtg tcggcagttg gttgccgact accgtaaccg cgccgcttaa cagcattcat
     7861 gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc gcaagtgctg
     7921 gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca tccgccggaa
     7981 ttgtttcgcg agcttgaaat tgccgagctg gatcaattga ttgccgtttc tcagcacacc
     8041 ttacgcgtgg tagcgctggc accggaaaaa gagggggcat tgcaggccat ccgccatctt
     8101 aaacagcaaa atgtacgagt gatgctgggg catagcgcgg cgacctggca acaaactcgc
     8161 gccgcgtttg atgctggtgc cgacggcctg gtgcattgct ataacgggat gacaggttta
     8221 catcaccgcg aaccgggaat ggttggcgcg ggattaacgg acaagcgcgc ctggctggaa
     8281 ctgatagccg atggtcatca tgtgcatccg gcggcgatgt cgctgtgttg ttgctgtgca
     8341 aaagagagaa tcgtgctgat caccgacgcg atgcaggcag ccgggatgcc ggatggtcgc
     8401 tatacgttat gtggcgaaga agtgcagatg cacggtggcg ttgtccgtac cgcgtccggt
     8461 gggctggcgg gcagtacgct gtctgttgat gcggcagtgc gcaacatggt cgagttgacg
     8521 ggcgtaacgc ctgcggaagc cattcatatg gcatcgctgc atccggcgcg aatgctgggt
     8581 gttgatggtg ttctgggatc gcttaaaccg ggcaaacgcg ccagcatcgt tgcgctggat
     8641 agcgggctgc atgtgcaaca aatctggatt cagagtcaat tagcttcgtt ttgatatttt
     8701 gctcctttat tgggccttca cttcccccgt aaggcctttc tttttctttc gttttgatct
     8761 gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg acctacggta cgccgcatcc
     8821 agcaatcggt gaacatagcg acgtcattac gcaattcttt aatcttccct tgtcttttct
     8881 ttcaatgatc acagatttcg ttttatttcc tttttctcca ttgaactttc ggtttctttt
     8941 ctatagattt taatcaacga aagacatcac caagtgaaat gaaacgaaag gcaagtgaaa
     9001 gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg agttatgcca gaaaattaca
     9061 cccctgctgc cgccgcaacc ggtacatgga ctgaagaaga gatccgccat cagcctcgcg
     9121 catggatccg ttcactcacc aacatcgacg cgctacgttc tgcgctcaat aacttccttg
     9181 aaccgttact gcgcaaagag aatctgcgga tcatcctgac cggagccgga acctcggcat
     9241 ttatcggtga catcatcgcg ccgtggctcg ccagccatac cggtaaaaac ttcagcgccg
     9301 taccgaccac cgatctggtc accaatccga tggactacct gaacccagct catccgctgc
     9361 tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgctgcc gtggaactgg
     9421 caaatcaatt tgtaccagaa tgctatcacc tgccgatcac ctgcaacgaa gcgggcgctc
     9481 tttaccaaaa cgcgatcaac agcgataacg cgtttgccct gctgatgccc gcagaaacgc
     9541 acgatcgcgg ctttgcgatg accagcagca ttaccaccat gatggccagc tgcctcgcgg
     9601 ttttcgcacc tgagacgatc aacagccaaa ccttccgcga cgtggcggat cgttgccagg
     9661 cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca ccgtggaaac
     9721 ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg gcgctgaaag
     9781 tgctggaact gactgcgggt aaactggcgg ccttctatga ttccccgacc ggattccgtc
     9841 atggcccgaa atcactggtc gataacgaaa cgctggtggt ggtatttgtc tcaagccacc
     9901 cttacacccg tcagtatgat cttgatctgc tggctgaact ccgccgtgac aaccaggcaa
     9961 tgcgcgtaat cgccatcgcc gcggaaagca ccgacatcgt cgctgccggt ccacatatta
    10021 tcctgccgcc gtcacgtcac tttatcgacg ttgagcaggc attttgcttc ctgatgtacg
    10081 cccagacgtt tgcactgatg cagtcgctgc acatgggcaa tacgccggat accccatcag
    10141 ccagcggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg caggcataag
    10201 aggatcgcat tatgagcatt atctccacta aatatctgtt acaggacgcc caggccaatg
    10261 gctacgcggt gcctgctttt aacattcata acgccgagac gatccaggcg atcctcgaag
    10321 tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac gccggggacc tttaaacata
    10381 tcgcgctgga agagatctac gccctgtgca gcgcctattc cacaacctac aacatgccac
    10441 tggcgctgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa gtccacgcag
    10501 gtgtgcgcag cgcaatgatc gacggcagcc acttcccgtt tgccgagaac gtgaagctgg
    10561 tgaaatcggt tgttgacttc tgccactcac aggattgcag cgtggaagca gaactgggcc
    10621 gcctgggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc ctgaccgatc
    10681 cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg gtagcgattg
    10741 gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg ctggcggaaa
    10801 ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc cagcgatgtt ccggatgaat
    10861 ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca gaattaaaaa
    10921 tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccgcagggt aatgatcctc
    10981 gttattatat gcgcgtcgga atggatgcga tgaaagaagt tgtcagaaat aaaattaatg
    11041 tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat ttatcccaat
    11101 gtacttcccg attaatatcg gggagtgcct taatggaaaa ggagatgact aaaccttaat
    11161 aaat
//
LOCUS       DAZFVO020000154        11009 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFVO020000154 DAZFVO020000000
VERSION     DAZFVO020000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.155"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            192..920
                     /gene="gadW"
                     /locus_tag="ACHKNB_RS19970"
                     /old_locus_tag="pgaptmp_003994"
     CDS             192..920
                     /gene="gadW"
                     /locus_tag="ACHKNB_RS19970"
                     /old_locus_tag="pgaptmp_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HGU4197410.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1283..4396)
                     /gene="mdtF"
                     /locus_tag="ACHKNB_RS19975"
                     /old_locus_tag="pgaptmp_003995"
     CDS             complement(1283..4396)
                     /gene="mdtF"
                     /locus_tag="ACHKNB_RS19975"
                     /old_locus_tag="pgaptmp_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HGU4197411.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTRARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGQSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4421..5530)
                     /gene="mdtE"
                     /locus_tag="ACHKNB_RS19980"
                     /old_locus_tag="pgaptmp_003996"
     CDS             complement(4421..5530)
                     /gene="mdtE"
                     /locus_tag="ACHKNB_RS19980"
                     /old_locus_tag="pgaptmp_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HGU4197412.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5638..5916
                     /locus_tag="ACHKNB_RS19985"
                     /old_locus_tag="pgaptmp_003997"
     CDS             5638..5916
                     /locus_tag="ACHKNB_RS19985"
                     /old_locus_tag="pgaptmp_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197413.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5917..6444)
                     /gene="gadE"
                     /locus_tag="ACHKNB_RS19990"
                     /old_locus_tag="pgaptmp_003998"
     CDS             complement(5917..6444)
                     /gene="gadE"
                     /locus_tag="ACHKNB_RS19990"
                     /old_locus_tag="pgaptmp_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HGU4197414.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7243..7815)
                     /gene="hdeD"
                     /locus_tag="ACHKNB_RS19995"
                     /old_locus_tag="pgaptmp_003999"
     CDS             complement(7243..7815)
                     /gene="hdeD"
                     /locus_tag="ACHKNB_RS19995"
                     /old_locus_tag="pgaptmp_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HGU4197415.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            8070..8402
                     /gene="hdeA"
                     /locus_tag="ACHKNB_RS20000"
                     /old_locus_tag="pgaptmp_004000"
     CDS             8070..8402
                     /gene="hdeA"
                     /locus_tag="ACHKNB_RS20000"
                     /old_locus_tag="pgaptmp_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HGU4197416.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8518..8844
                     /gene="hdeB"
                     /locus_tag="ACHKNB_RS20005"
                     /old_locus_tag="pgaptmp_004001"
     CDS             8518..8844
                     /gene="hdeB"
                     /locus_tag="ACHKNB_RS20005"
                     /old_locus_tag="pgaptmp_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HGU4197417.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8908..9555
                     /gene="yhiD"
                     /locus_tag="ACHKNB_RS20010"
                     /old_locus_tag="pgaptmp_004002"
     CDS             8908..9555
                     /gene="yhiD"
                     /locus_tag="ACHKNB_RS20010"
                     /old_locus_tag="pgaptmp_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGU4197418.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTTAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9597..10127)
                     /gene="dctR"
                     /locus_tag="ACHKNB_RS20015"
                     /old_locus_tag="pgaptmp_004003"
     CDS             complement(9597..10127)
                     /gene="dctR"
                     /locus_tag="ACHKNB_RS20015"
                     /old_locus_tag="pgaptmp_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HGU4197419.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVNFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10283..10849)
                     /gene="slp"
                     /locus_tag="ACHKNB_RS20020"
                     /old_locus_tag="pgaptmp_004004"
     CDS             complement(10283..10849)
                     /gene="slp"
                     /locus_tag="ACHKNB_RS20020"
                     /old_locus_tag="pgaptmp_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HGU4197420.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3006 a         2502 c         2529 g         2972 t
ORIGIN      
        1 agctatacgc agtgcgaaaa tatattcgtg ctgcatttac ttattatcaa ttaactgtta
       61 tgcaaaacta ctttgtggat aaattttggt cctaccaaat ctggcagttt tttcgctaag
      121 aaacagtctg gcaacatttc attagtatac tgaaattgaa ataatcgcag tatgaaatat
      181 aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt
      241 atcatgaaca gcataaaata tcgctgcata acgagagtat cgtgctgctg gagaaaaatt
      301 tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc gatgagctga
      361 cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg ttacatagca
      421 aagaccgtct attaattaac cagtcacccc ccatgccgct ggtgacggcg atttttgata
      481 gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac ctttcctgtt
      541 tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc atcagcactg
      601 tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg tatctgcgcg
      661 atatcgcgga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg caggatgaaa
      721 atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga cgattactcg
      781 agttacgtca aattcctctg catactattg cagaaaaatg tggctatagc agtacgtcgt
      841 actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt gcgcaacatt
      901 cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat ctcacacctg
      961 cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt ctctttcgca
     1021 gtttgccagg cggtataggt attttcagga ggaaaagact cggcatgttt gggattatta
     1081 agctgacaat tcatacgatt acgggaatgt gggtcagtat tgtgctgttc aaattgatga
     1141 cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta ttcctgacgg
     1201 ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa ccttcacgtt
     1261 caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga tgttccacta
     1321 caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac atcccgccca
     1381 tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg ataaccagcg
     1441 gcagcacgcc gagaataaag gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg
     1501 ccgcttcgat gattgcctct atcggcgttt tcccttcttt ttgcatcatc tcaacggcaa
     1561 attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct
     1621 ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc
     1681 ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag agtgcggcga
     1741 gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg tttgaggata
     1801 acgcttcctg atacgactgt ccggtccatg agtagccgac gcctgccgga agtttagcga
     1861 ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc gcttcaccta
     1921 aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca taggtccatt
     1981 cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt acataccatt
     2041 ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac acttttttca
     2101 cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct
     2161 ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg acgttcactt
     2221 tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt
     2281 gcgctgccag tgataacagc tcgtttcgtg cccgggttag tttttcgtgc cccaggttac
     2341 cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc accgcgggta
     2401 agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt gcccgctgaa
     2461 tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg agactgataa
     2521 acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg
     2581 actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc agcactttcg
     2641 tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat accccctgat
     2701 cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa
     2761 tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg
     2821 tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt
     2881 tgtgaccgcc ttccggcgcg gctttcagaa tggtggcgca cagggcaggg gtcaggctca
     2941 ttgccacaaa tactgaaagc agcatggagg agatcagcgt gatggagaac tggcggtaga
     3001 tctccccggt tgcgccgctc ataaaggcca tcggcataaa cactgcggaa agaacaacag
     3061 caataccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct tccttcggtg
     3121 gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg gcgtcatcca
     3181 ccagtaaccc tatcgccagc accatcccga acatagttaa cgtgttgatg gtgaaaccga
     3241 ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatc gtcgggatga
     3301 ttgtggcacg gaaattctgc aaaaacagat acatgaccag gaagacgagg atgatagcct
     3361 caaccagtgt tttaaaaact tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag
     3421 gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc tcctctttga
     3481 ctgcccgcga ggtatccagg gcgtttgctc cggtagccag tttgatggcg atcccggcag
     3541 caggtttgcc gttatagcgc gccacggtgg aataatcttc cgctccaagt tcgacgcggg
     3601 cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg attttgccaa
     3661 attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt tgctggtctg
     3721 ccgcctgtgg catgccaccc agttgaccac cggaaatctg gttgttttgc accttaatct
     3781 gggaaataac atcggaaggt accagattgt atttattgag tttttgcggg tccagccaga
     3841 tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg gtacggctta
     3901 gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg aggctgccgt
     3961 tatcagaaat aaacgccgct accatcagaa tattactgct cgacttatca acgctaatcc
     4021 cctgctgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta ttttgcacct
     4081 gaacctgtgc gatatcagga gatgtcccag tctcgaaggt cagagtgata gaggcattac
     4141 ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc atattttgct
     4201 caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacctgga taggtagcgc
     4261 tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg
     4321 ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc gggcgatcaa
     4381 taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc gattcggtgc
     4441 tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga atgcgttgca
     4501 aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc cactggtcgc
     4561 cgatggcttt gctggcttca atttcgcgta gctgcacaac atcgtcttta tccagaatga
     4621 gcgccgttgc tttaccctgg gcgttgtggg tgacgccttc ctgcggcacc agtaatacat
     4681 tctggcggct accttcatcc actaatgccg tgacgtacat gccaggcagc aagtcaccat
     4741 ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt
     4801 cggagaattt cagcgtgccg gtctggctgt agcgtttacc attttccaga ttgagctgta
     4861 ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct ttcatgcgta
     4921 agaaatcttg caccgactgc gtgagatcga cataaatcgg gtccagacgt tgtacggtaa
     4981 ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc
     5041 tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc gcctgttcaa
     5101 cagccgcttt ggcgacggtg acattggctt ctgcttcatt caactgggtg cgcgcggtat
     5161 cgtaatcctg acggctaacg tagttggtct tcagcaacga tgcctggcgg ttaaaggtga
     5221 tgcgggcatt gctggcggta gagagcgctt tcgccagcga gcctttggcg gagtttagct
     5281 cggcctgtaa aggtgcagga tcaatctgat acagcgaatc gccctggttc actttatcgc
     5341 cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc tcggcaactt
     5401 cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg
     5461 tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg
     5521 cggtgagcat cgcgccgcag aataacaacg gtattaacag ctttcttctt ctgttcattt
     5581 tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag gagagatatg
     5641 agggatttac agaccagcgg catagtgggg ttgtcagcca gtaaggtggg atacaggcac
     5701 agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc aacagttcct
     5761 gccagcattc gggcaacgaa gggttctact ggtggataca cataccaggg gaataaagtc
     5821 gttttagcct ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg catccctcgt
     5881 catgccagcc atcaatttca gttgcttatg tcctgactaa aaataagatg tgatacccag
     5941 ggtgacgatg tcgctcatac gtcttagttt cagcttgtac ataatgttga atttctgaat
     6001 ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat ttttattttt
     6061 cagaatcagt tgcataattt tgaactgatt atgtgaaaaa cataattgtg aattcttatg
     6121 gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt tgcggctaac
     6181 aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca aaacaataat
     6241 tctctcggca tctaatttct ccagaaattt aatcgcttct tcatcaagga tatgattgtg
     6301 ataggtatca atgataacct tgaagggttt attgcctact tttttgatct ctgacaggga
     6361 attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta aaagaaaaga
     6421 atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt cgaacatgtc
     6481 atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa acgcctataa
     6541 gaactttgcc cttcgtgaac taatacgtct attaacacta atcaatactt cagatttatc
     6601 catcacgcac tccatatatt gatcctaagt aagattcctg gttgttatca atttgtaaat
     6661 atcaaacaaa aagttaacgt ttgcggatta ctgcccaaga ataagttatc aataaccagt
     6721 ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct
     6781 ccttgtttcg acttaagctg gcaattggat tgccagcttt cttttaacgc attgaaatta
     6841 cctgagtaaa taacgcattc atgtcttcag gtttttatat agcgtctgga gacacggata
     6901 tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact tatttaaccg
     6961 cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact tttaaaacat
     7021 taaacctgac tacggaaaat atcagccatg ataatgtttg aaatggctaa tttgccatgg
     7081 agtgaaaaaa attagatgaa attcagtagg ttgaaatgat cactagcagg taattatttc
     7141 aatgatagtg cgcaattgat ctacaacact gcgtagcgga gagagtatta atcggatcat
     7201 agtcacatca agtgactatg atccgggtga caaccgggat aattattgct gcttaacgaa
     7261 caaactggcg aagctgaaca ggctggcggc gctaaatatc agttcaattc ccaccagtgt
     7321 ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg aatatccagg caatgacgat
     7381 atccagcaca ccaataacga gctgtagcca gctgcctttc attgaacgct gacgatacca
     7441 actcatcagg cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc
     7501 aaaaatgccc agctccggtg cgcggatgaa gaaatagccg atcaataaat aggcgactgc
     7561 gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga ataacccgac
     7621 aataagcgca atacccgagc agattaataa tgcacccact actgtgctta aaatatcgcc
     7681 agagacgaac gggaaactga tacacagcaa cccgacgata aacagcagca cggcaataaa
     7741 ctggattgct ctgcgatgct ttttcagcat ctccagatca aacttcaaaa ttgttgcctt
     7801 atctatatat aacatagaac caccctataa aattaagaag aaaatcccct gctatcaatc
     7861 tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa aaatttccta attgcagtat
     7921 ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat attttccatc
     7981 aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat tcgtgacggc
     8041 tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt attcttggtg
     8101 gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca gctgataaca
     8161 aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa tccttccagc
     8221 caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa gatgcggttt
     8281 tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt actcaggata
     8341 aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag aaagatatgt
     8401 aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg aatatcttct
     8461 ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg ttacaaaatg
     8521 aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc tttgtcactg
     8581 gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac ctgccaggaa
     8641 tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct gcatgaagaa
     8701 acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac tcaaattcct
     8761 aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa
     8821 gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca agttgagcca
     8881 tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg tcttatactc
     8941 gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg
     9001 ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt ttcgaaatat
     9061 ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg tattgctgct
     9121 caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat
     9181 attgtaggtc tgacgacggc agcggatatc tgggtgacca ccgccatagg tatggttatt
     9241 ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgtt ggtgctggaa
     9301 gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca gctgacatta
     9361 gtgaacggta atacggtatc aatgctcgac tggtttaagc agcaaaaaat aaaaacggat
     9421 ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat ccagcttcat
     9481 gccacaacgt cgatagaaga cctgttgcga ttattaaaag gtatggcagg ggtgaaaggc
     9541 gtttcaatta gctaatctgt gtggcagcgt agccagactc accgtaagcc tgaaattcac
     9601 accagataat caatatgctg atggcgtacc agctcattga tccgcttaag attgaggatc
     9661 atcatgatat tgtgtttatg gcagtagaac gtttttagcg atattttcag tttctccaga
     9721 atatcttccg ttgtcatttg attcgcaatg cagctcagaa cctgatgttg cttgcgcgaa
     9781 agtgacacag ccgcttcagg cacagagcag taactgccat tgatgagtgc gacaaaatca
     9841 atgatagtga tagtccgcgg aacaaagttc accggcactt tccccaggca gcgtttaata
     9901 ttaaaggggg agaaaataat cacatggaca ggttttaatc gttcaatttg agtgagaaaa
     9961 ttagaatgaa atacattatt cattaatgta tcaataatga cgaaggcatt ctgatgcaac
    10021 attacgtcga tctcttcttc gttctgtata tgaacgacat tacctttact cagaatgttt
    10081 ttcatcgcgg tgaagaacat cgtatccctg gtaattataa gaaacataac cgactcctgg
    10141 tgcggacatt aactttataa tcagtttgcc aaaggtaatt aacaggacag ggcttataat
    10201 aataatagtt agcttactgt atattaattt tatgtaatac atgatgcaga tgctgagaaa
    10261 gcatctgcat ctttcggtgg tgttatttga ccagctcagg tgttacctga ctcaccgcat
    10321 tggtgtagta aggcgcaccc cagcccggtt ccggccagaa tgcaccatag ccgtaatccc
    10381 acaggttata ggtggtatta accacttctc tcaaatgcca cacctggatg ccctgcatat
    10441 tcacttccag gaagttatac gggactttat tgataaagcc tggttgttca ccctgaatgg
    10501 taccgaggat ggtaacaaag tgattacgat agttcactgg atcaaggaag ccgctttgtc
    10561 tggcgagcag tcggccctga tagttggctt caatatcagg cttcgcatag ctatccaacg
    10621 gtaatacagc gatttctaac aacgtatccg ttttgccatt gataacgttg ataaccttcc
    10681 caccaaagcg cgcttgttga ccaacatata accccggctg gttatgaaca gcaacaaaac
    10741 ttttttgaat atcaggttgg ttattgcctt tgatattttg cggaattgaa ctacatgcgg
    10801 caagcaaaaa tgaaaggctg aggatgagtg caccttttgt catgttcatg ttactatcct
    10861 tatcaacaaa tattaaaacc ataatagata tttctttata gttttcatct gattctgagg
    10921 tgatatgcct gatagtaacg taaacctgtc tgatgtttat atcccttctg tttagttcaa
    10981 ttccgttatt ttggggcgtg gtatgagta
//
LOCUS       DAZFVO020000155        10998 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFVO020000155 DAZFVO020000000
VERSION     DAZFVO020000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.156"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            90..851
                     /gene="adiY"
                     /locus_tag="ACHKNB_RS20025"
                     /old_locus_tag="pgaptmp_004005"
     CDS             90..851
                     /gene="adiY"
                     /locus_tag="ACHKNB_RS20025"
                     /old_locus_tag="pgaptmp_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HGU4197421.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            988..2325
                     /gene="adiC"
                     /locus_tag="ACHKNB_RS20030"
                     /old_locus_tag="pgaptmp_004006"
     CDS             988..2325
                     /gene="adiC"
                     /locus_tag="ACHKNB_RS20030"
                     /old_locus_tag="pgaptmp_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HGU4197422.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2429..4072
                     /gene="eptA"
                     /locus_tag="ACHKNB_RS20035"
                     /old_locus_tag="pgaptmp_004007"
     CDS             2429..4072
                     /gene="eptA"
                     /locus_tag="ACHKNB_RS20035"
                     /old_locus_tag="pgaptmp_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HGU4197423.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4069..4737
                     /gene="basR"
                     /locus_tag="ACHKNB_RS20040"
                     /old_locus_tag="pgaptmp_004008"
     CDS             4069..4737
                     /gene="basR"
                     /locus_tag="ACHKNB_RS20040"
                     /old_locus_tag="pgaptmp_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HGU4197424.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4738..5838
                     /gene="pmrB"
                     /locus_tag="ACHKNB_RS20045"
                     /old_locus_tag="pgaptmp_004009"
     CDS             4738..5838
                     /gene="pmrB"
                     /locus_tag="ACHKNB_RS20045"
                     /old_locus_tag="pgaptmp_004009"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HGU4197425.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5814..5903)
                     /gene="pmrR"
                     /locus_tag="ACHKNB_RS20050"
                     /old_locus_tag="pgaptmp_004010"
     CDS             complement(5814..5903)
                     /gene="pmrR"
                     /locus_tag="ACHKNB_RS20050"
                     /old_locus_tag="pgaptmp_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HGU4197426.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6015..7517)
                     /gene="proP"
                     /locus_tag="ACHKNB_RS20055"
                     /old_locus_tag="pgaptmp_004011"
     CDS             complement(6015..7517)
                     /gene="proP"
                     /locus_tag="ACHKNB_RS20055"
                     /old_locus_tag="pgaptmp_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HGU4197427.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7781..8658)
                     /gene="rdcB"
                     /locus_tag="ACHKNB_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /pseudo
     CDS             complement(7781..8658)
                     /gene="rdcB"
                     /locus_tag="ACHKNB_RS20060"
                     /old_locus_tag="pgaptmp_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            complement(8655..10883)
                     /gene="crfC"
                     /locus_tag="ACHKNB_RS20065"
                     /old_locus_tag="pgaptmp_004013"
     CDS             complement(8655..10883)
                     /gene="crfC"
                     /locus_tag="ACHKNB_RS20065"
                     /old_locus_tag="pgaptmp_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HGU4197428.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2747 a         2862 c         2705 g         2684 t
ORIGIN      
        1 tatattcatc atacaattat ataaccattt cccggtatcg ctttgcttta gcgagaactg
       61 gtcttttatt cgcactcagg agtacatgta tgaggatttg cagcgaccaa ccttgtattg
      121 ttttattgac tgaaaaagat gtctggataa gggtgaatgg gaaagaacct attagcctta
      181 aagctaacca tatggcgtta ttaaattgtg aaaataatat tatcgacgtc tcctctctta
      241 acaacacttt ggttgctcat attagtcacg acatcatcaa agattacctc cggtttctga
      301 ataaagatct ctcgcaaata ccagtatggc aacgtagcgc tacgcccata ctcaccctgc
      361 catgcctgac gccagacgtc tttcgcgttg ccgcgcaaca cagcatgatg cccgcagaaa
      421 ctgagtcaga aaaggaacga acacgcgcat tattattcac tgtgctatcc cgttttctcg
      481 acagtaaaaa attcctttca ttaatgatgt atatgttacg taattgtgta agtgacagcg
      541 tttatcaaat tattgaaagc gatattcaca aagactggaa tcttagtatg gtagccagtt
      601 gtttatgtct tagcccaagt ctgttaaaga aaaagttgaa aagcgaaaac accagttata
      661 gccaaataat caccacctgc cgcatgcgtt atgccgtaaa tgaattaatg atggacggta
      721 aaaatatctc tcaggtatca cagtcctgcg gctacaacag tacgtcgtac tttatttctg
      781 tctttaaaga cttctacggt atgacgccgc tgcattatgt tagtcagcac agagaacgca
      841 ctgtcgcctg atttttaacc ttaacgaaga gctacattaa taacggcatc agcgataacc
      901 cggtcgataa taattcaact atcgaatgca ggcgtatgat atgacgtaat ttattgtcac
      961 gaagctcgcc ttcgcaggag tttaattatg tcttcggatg ctgatgctca caaagtgggc
     1021 ttaatccccg tcaccctgat ggtgtcgggg aatattatgg ggtcaggtgt ttttctgcta
     1081 cctgcaaacc tggcctccac tggcgggatt gccatttatg gatggttggt gacgattatc
     1141 ggtgcgctgg ggctctcgat ggtatacgcc aaaatgtcgt tcctcgatcc aagtcctggt
     1201 ggttcttacg cttacgcccg ccgctgcttt ggcccgtttc tcggttatca aaccaacgtc
     1261 ctctactggc tggcctgctg gatcggcaat atcgccatgg tggtcattgg cgtaggatat
     1321 ttaagttact tcttcccgat tctgaaagat ccattggtat taaccatcac ctgcgtcgtg
     1381 gtgctgtgga tcttcgtcct gctgaacatt gtcggtccga aaatgatcac ccgtgtgcag
     1441 gcagttgcca ccgtgctggc gctgatcccc atcgtcggga ttgccgtatt tggctggttc
     1501 tggttccgtg gtgaaaccta tatggcggca tggaacgtca gcggcctggg caccttcggt
     1561 gcaattcaaa gtacccttaa cgttacgctg tggtcgttca tcggtgtgga aagtgcctcc
     1621 gttgccgcag gtgtggtgaa aaacccgaaa cgcaatgtcc ctatcgccac cattggtggg
     1681 gtattgattg ccgccgtttg ctatgtactt tctaccaccg cgattatggg gatgatccct
     1741 aatgccgcac tgcgcgtttc tgcttcgcca ttcggtgatg ccgcacggat ggcgttgggt
     1801 gacaccgccg gggccattgt ttccttctgc gcagctgcgg gttgcttagg ttcactgggc
     1861 ggctggacgt tgctggcggg tcaaacggcg aaagccgctg ccgatgacgg actgttccca
     1921 ccgatttttg cccgtgtaaa taaagcgggc acgccagtgg cggggctgat tatcgtcggt
     1981 attttgatga ccatcttcca gctcagcagc atttcaccaa acgcgaccaa agagttcggt
     2041 ctggtttctt ccgtctcggt catctttaca ctggtgccat atctttacac ctgtgcggcg
     2101 ttactgctgc tcggtcacgg tcactttggt aaagcacgcc cggcatatct ggctgttact
     2161 accattgcct tcctctactg catttgggcc gtggtggggt ccggggcgaa agaggttatg
     2221 tggtcatttg tcaccctgat ggtaatcacc gccatgtatg cgctgaatta caaccggctg
     2281 cataaaaacc cgtatccctt agatgcacca ataagcaaag attaattccc cttaatccag
     2341 caaacataaa agccaacctt aagaacttaa ggttggctta attttgcttt gcgagcatat
     2401 gcgcactttg ttcgatggaa acaccgtgat gttgaagcgc ctactaaaaa gaccctcttt
     2461 gaatttactc gcctggctat tgttggccgc tttttatatc tctatctgcc tgaatattgc
     2521 cttttttaaa caggtgttgc aggcgctgcc gctggattcg ctgcataacg tactggtttt
     2581 cttgtcgatg ccggtcgtcg ctttcagcgt gattaatatt gtcctgacac taagctcttt
     2641 cttatggctt aatcgaccac tggcctgcct gtttattctg gttggcgcgg ctgcacaata
     2701 tttcataatg acttacggca tcgtcatcga ccgctcgatg attgccaata ttattgatac
     2761 cactccggca gaaagttatg cgctgatgac accgcaaatg ttattaacgc tgggattcag
     2821 cggcgtgctt gctgcgctga ttgcctgctg gataaaaatc aaacctgcca cctcgcgtct
     2881 gcgcagtgtt cttttccgtg gagccaatat tctggtttct gtactactga ttttgctggt
     2941 cgccgcactg ttttataaag actacgcctc gttgttccgc aataacaaag agctggtgaa
     3001 atccttaagc ccctctaaca gcattgttgc cagctggtca tggtactccc atcagcgact
     3061 ggcaaatcta ccgctggtgc gaattggtga agacgcccac cgcaacccgt taatgcagaa
     3121 cgaaaaacgt aaaaatttga ccatcctgat tgtcggcgaa acctcgcggg cggagaactt
     3181 ctccctcaac ggctacccgc gtgaaactaa cccgcggctg gcgaaagata acgtggtcta
     3241 tttccctaat accgcatctt gcggcacggc aacggcagtt tcagtaccgt gcatgttctc
     3301 ggatatgccg cgtgagcatt acaaagaaga gctggcacag caccaggaag gcgtgctgga
     3361 tatcattcag cgagcgggca tcaacgtgct gtggaatgac aacgatggcg gctgtaaagg
     3421 tgcctgcgac cgcgtgcctc accagaacgt caccgcgctg aatctgcctg gtcagtgcat
     3481 caacggcgaa tgctatgacg aagtgctgtt ccacgggctg gaagagtaca tcaataacct
     3541 gcagggtgat ggcgtgattg tcttacacac catcggcagc cacggtccga cctattacaa
     3601 ccgctatccg ccgcagttca ggaaatttac cccaacctgc gacaccaatg agatccagac
     3661 ctgtagcaaa gagcaactgg tgaacactta cgacaacacg ctggtttacg tcgactatat
     3721 tgttgataaa gcgattaatc tgctgaaaga acatcaggat aaatttacca ccagcctggt
     3781 ttatctttct gaccacggtg aatcgttagg tgaaaatggc atctatctgc acggtctgcc
     3841 ttatgccatc gccccggata gccaaaaaca ggtgccgatg ctgctgtggc tgtcggagga
     3901 ttatcaaaaa cggtatcagg ttgaccagaa ctgcctgcaa aaacaggcgc aaacgcaaca
     3961 ctattcacaa gacaatttat tctccacgct attgggatta actggcgttg agacgaagta
     4021 ttaccaggct gcggatgata ttctgcaaac ttgcaggaga gtgagtgaat gaaaattctg
     4081 attgttgaag acgatacgct gttattgcag ggattgattc tggcggcgca aactgaaggc
     4141 tacgcgtgcg atggcgtgac aaccgcgcgg atggcggaac aaagccttga ggccggtcat
     4201 tacagcctgg tggtactgga tttagggtta cccgacgaag atggactgca ttttctcgcc
     4261 cgtatccggc agaaaaaata caccctgcca gtactgatcc tcaccgctcg cgatacgctg
     4321 accgacaaaa tcgctgggct ggatgtcggt gccgatgact atctggtgaa gccttttgcg
     4381 ctggaagagt tacacgcccg tatccgcgcc ctgctacgac gccataataa tcagggcgaa
     4441 agtgagctga ttgttggcaa tctgacgctg aatatgggtc gccgtcaggt atggatgggc
     4501 ggtgaagagt tgattctgac gcccaaagaa tatgctctgc tgtcacggtt aatgctcaaa
     4561 gcaggcagtc cggtgcatcg ggaaattctc tacaacgaca tctataactg ggacaatgaa
     4621 ccctcgacca acaccctgga agtgcatatc cacaatctgc gcgacaaagt gggcaaagcc
     4681 cgtatccgca ccgtgcgcgg ctttggctat atgctggtcg cgaatgagga aaactaattg
     4741 aatctgatgc attttctgcg ccgaccaata tcgctgcgcc aacggctgat attgaccatc
     4801 ggggccattt tgttggtgtt tgagctgatc agcgtcttct ggctatggca tgaaagtacc
     4861 gagcagattc agctgtttga gcaggcgcta cgcgacaatc gcaacaacga tcgccacatc
     4921 atgcgtgaga tccgcgaggc ggtcgccagc ctgattgtcc ccggcgtctt tatggtcagc
     4981 ctgacgctat ttatctgcta tcaggcggta cgccgcatca ctcgcccgct ggcggagctg
     5041 caaaaagagc tggaagcgcg caccgccgac aacttaacgc ccattgccat tcacagcgcc
     5101 accctcgaaa tcgaagcggt ggtttcggcg ttgaacgatc tggtcagtcg cctgaccagc
     5161 acgctggata acgaaaggtt gtttaccgct gacgtcgcgc acgaactgcg aacgccactg
     5221 gcgggggtgc gtttgcatct ggaactgctg gcgaaaacgc atcacattga tgtagcaccg
     5281 ttagtggcac ggcttgatca gatgatggag agcgtctccc agctgctgca actggcgcgt
     5341 gccggacagt cattttcttc cggtaattat caacatgtaa aactgctgga agatgtcatt
     5401 ctcccctcgt atgacgaact cagtaccatg ctcgaccagc gacagcaaac cctgctattg
     5461 ccagagagcg ccgccgacat caccgttcag ggtgatgcaa ccctgctgcg gatgttattg
     5521 cgaaacctgg tagaaaacgc ccatcgttac agcccgcaag gcagcaacat tatgattaag
     5581 ctgcaagaag atggcggagc ggtcatggca gttgaagatg aaggaccagg tattgatgag
     5641 agtaaatgcg gggagttgag taaagcgttt gtacgtatgg acagccgtta tggcgggatt
     5701 ggtctggggt taagtattgt cagccgcatt acacagttgc atcacgggca gtttttcctg
     5761 caaaaccggc aagagacttc cggcacgcgg gcctgggtac ggctgaagaa agatcagaac
     5821 gtggcaaacc agatataaag aaagctgctg accaccagca cgctgaacac ggtagttaaa
     5881 ctttcataaa cacggttttt cattgcactc tcctccgtta acctggagga gagtatgcgc
     5941 gtcagagatt aagtgaacct taagagttca attggcctga taagacagcg tcacatcagg
     6001 ccatccgttt cagcttattc atcaattcgc ggatgttgct gcaccaggcg ggtacgtttc
     6061 gcctgcaaat cggcaatctc gtggtcaata ttatcgattt tctgctcgat attatcgtaa
     6121 tgctcgacga gaatttcctt cgcttcctgt atatctgacg ccgccggtgt cgcacctttc
     6181 aacggacgat ttgccgtctc tttcatggtt acgccggtga ttaaaccaat caccgccact
     6241 accatcaggt aataggcagg catcatcaga ttctgcgagc tttcgaccag ccaggccgcc
     6301 agtgttggcg tcagaccggc aaccagcacc gaaatattaa atgccgccgc cagcgcgctg
     6361 taacggatat gcgtcgggaa catcgctggc aaggtagaag ccataacgcc cgtaaagcag
     6421 ttaaggatca ccgccagcat cagtaacccg gcaaaaatca ggccgatgac gttactgtta
     6481 atcagaataa acgccgggat cgccaacaca aacagggcaa cactaccaag tagcacaaac
     6541 ggacgacggc caaaacggtc actcagcaag cccatcaccg gctggacaaa cagcataccg
     6601 atcataatgg cgataataat cagcaccccg tggtcttcgg agtaatgcag gttatgcgac
     6661 aaataactcg gcatataggt cagcaacatg tagtaagtca cgttggtggc aattaccaga
     6721 ccaatacatg tcaacaggct gcgccagtgt ttggtggcaa tctctttaaa cgagactttc
     6781 gggccatcct gcaaaccttc gcggtcgccc tgttccagtt tatcgacatg ctgctggaac
     6841 gccggagttt cttccagcgc atggcgcagg taaagcccga taatccctaa cggcagagca
     6901 ataaagaacg gaatacgcca gccccagtcg aggaagttcg cttcgccgac aatggtcgaa
     6961 attaacacca ctacgccagc acccagcaca aacccggcaa tagaaccgaa gtccagccag
     7021 ctgcccataa agccacgttt acggtcaggt gagtattccg caacaaatat cgacgccccg
     7081 gtatattcac cgccgaccga gaaaccttgt gccatcttac agatcaacag cagaatcggt
     7141 gcccaaatac caatcgtgtc gtaggacggt attaagccaa tacagaacgt actgatcgac
     7201 ataatcacaa tagtgatagc gaggatcttc tggcgaccat atttatcgcc caacatacca
     7261 aagaagagcc cgccaagcgg tcgaatcaga aagggaacgg agaacgtggc aagtgcagca
     7321 accatctgca cgctggggtc agcccccggg aaaaaaactt tacctaatgc gtaagcaaca
     7381 aaaccataaa caccaaaatc aaaccattcc attgcattac ccagtgatgc tgcggtaatg
     7441 gctttacgca gtttaccgtc atcaataatg gtgacatcac gaagggtaat cggttttact
     7501 tttttccttt tcagcataac tttcctcgca gagttggccc atttccgcaa acgccagaaa
     7561 aatcagcgcg acggccgcaa aaggggagcg ggaattgcag gatgcaatct ctgtaacaag
     7621 cgtagcgggt ttgcttacac cctccggtta aggaatgtac aatcaaacca gttgacgcct
     7681 gaattgggca gttaatgacc tttttaccct accagtgttt aaatttgtga tcaacttcac
     7741 acatggattt cgtttaggac tcattgatgt aactgattat ttacgccaaa tttacagaga
     7801 agatttcctc gaccattgcc cccgccagac gctgcgctga acagagtcgt ggcatcccca
     7861 gcgcgacagt ttgccagcgt tgggtcactg accagcaaac gggttgttta tctgccgtgg
     7921 tccactttcc cagccaactt aaaaaatcag catgatcccc gcccgtcgga acggtcgaag
     7981 aggcaaacca ctgatggtaa tagtgacgcg tggtcggggc cgcattgata ctctctgcca
     8041 gatgttgtgt cgctttcacg cgcaaatcac ccagcaacaa cgccaccgct tgcgggcttc
     8101 ccagacgaca ggcatcgcca aatacccccc aacgcaactc ttcgagaacg ataaaacagc
     8161 gcccaggcaa cgaccggcgt tatacgcccc agcacgccag cgggtaaaaa tttgctcgct
     8221 ctgttcaccc gcctgcacca gtaggcctcg ttgcgttaat gccttttcct tccagcgaca
     8281 acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg ggaaagcaga
     8341 gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct gcgtcagcgc
     8401 cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc gcaggctaag
     8461 cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg aaagaagttc
     8521 ctgcatcagt cgttcacgaa actgctgctg atgggcttgt ggaaggcggg catcatcgcc
     8581 ctgcgccaga tccactaaaa agcggggata aaccgactcc agcacgcgac cggggccgtc
     8641 cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc atcgcgtaac
     8701 agctgggcgt cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa ttgctgctgc
     8761 atcagcctga ccaccgattc attttgttga cgcagagcca ggctttcgcg taaacgcgtc
     8821 tgtaattccg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc tgccgtaaca
     8881 gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaataaag atcgacttct
     8941 gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga tttctcattt
     9001 ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac aacgcctaaa
     9061 cgcgtgctct gctcgtccgt tgcatcctgg cgcgaaatga ctgcactgaa cagctggcga
     9121 gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg acgaaaccct
     9181 gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc ggttaacacc
     9241 cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact gatggtgttc
     9301 tgcgccgtct gtaacagtgt ctcacagcgg gtacgcatct cgcttaacgg ctccgaatca
     9361 tcctgaaaca aggcggctaa ctgcgcattc agcgcatctt gttgttgacg cagaaagtgg
     9421 ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga
     9481 ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg caattgttca
     9541 caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa
     9601 ttcaacagtt tatgcgcagc agatcgcaaa gcatacaacg aggcgttagc gtaagcggca
     9661 tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca
     9721 tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca
     9781 tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc gttgttggct
     9841 aactcatggc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg aaatatctgc
     9901 tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg
     9961 tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag cggcaccgat
    10021 tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt cagttgcgta
    10081 taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc
    10141 agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga
    10201 taactctcca gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg
    10261 gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg cactaaatca
    10321 ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt ttcgaaagcg
    10381 acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg
    10441 gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat taaacaatcg
    10501 attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt atggcgaata
    10561 agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg
    10621 gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag caccatttcc
    10681 agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc gatattaggc
    10741 tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag aggcattttt
    10801 tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac
    10861 tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt gttactttta
    10921 tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca agaaataatt
    10981 ttatatttag tgtgttgt
//
LOCUS       DAZFVO020000156        10981 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFVO020000156 DAZFVO020000000
VERSION     DAZFVO020000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.157"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            188..397
                     /locus_tag="ACHKNB_RS20070"
                     /old_locus_tag="pgaptmp_004014"
     CDS             188..397
                     /locus_tag="ACHKNB_RS20070"
                     /old_locus_tag="pgaptmp_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197429.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            514..2835
                     /locus_tag="ACHKNB_RS20075"
                     /old_locus_tag="pgaptmp_004015"
     CDS             514..2835
                     /locus_tag="ACHKNB_RS20075"
                     /old_locus_tag="pgaptmp_004015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4197430.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAVDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(2892..3227)
                     /gene="ymgD"
                     /locus_tag="ACHKNB_RS20080"
                     /old_locus_tag="pgaptmp_004016"
     CDS             complement(2892..3227)
                     /gene="ymgD"
                     /locus_tag="ACHKNB_RS20080"
                     /old_locus_tag="pgaptmp_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HGU4197431.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3231..3575)
                     /gene="ymgG"
                     /locus_tag="ACHKNB_RS20085"
                     /old_locus_tag="pgaptmp_004017"
     CDS             complement(3231..3575)
                     /gene="ymgG"
                     /locus_tag="ACHKNB_RS20085"
                     /old_locus_tag="pgaptmp_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HGU4197432.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3577..3750)
                     /gene="ymgI"
                     /locus_tag="ACHKNB_RS20090"
                     /old_locus_tag="pgaptmp_004018"
     CDS             complement(3577..3750)
                     /gene="ymgI"
                     /locus_tag="ACHKNB_RS20090"
                     /old_locus_tag="pgaptmp_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HGU4197433.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3851..4036
                     /gene="ymgJ"
                     /locus_tag="ACHKNB_RS20095"
                     /old_locus_tag="pgaptmp_004019"
     CDS             3851..4036
                     /gene="ymgJ"
                     /locus_tag="ACHKNB_RS20095"
                     /old_locus_tag="pgaptmp_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HGU4197434.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3997..4116
                     /locus_tag="ACHKNB_RS20100"
                     /old_locus_tag="pgaptmp_004020"
     CDS             3997..4116
                     /locus_tag="ACHKNB_RS20100"
                     /old_locus_tag="pgaptmp_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4197435.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            4151..4495
                     /locus_tag="ACHKNB_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /pseudo
     CDS             4151..4495
                     /locus_tag="ACHKNB_RS20105"
                     /old_locus_tag="pgaptmp_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4867..5133)
                     /gene="minE"
                     /locus_tag="ACHKNB_RS20110"
                     /old_locus_tag="pgaptmp_004022"
     CDS             complement(4867..5133)
                     /gene="minE"
                     /locus_tag="ACHKNB_RS20110"
                     /old_locus_tag="pgaptmp_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HGU4197436.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5137..5949)
                     /gene="minD"
                     /locus_tag="ACHKNB_RS20115"
                     /old_locus_tag="pgaptmp_004023"
     CDS             complement(5137..5949)
                     /gene="minD"
                     /locus_tag="ACHKNB_RS20115"
                     /old_locus_tag="pgaptmp_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HGU4197437.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5973..6668)
                     /gene="minC"
                     /locus_tag="ACHKNB_RS20120"
                     /old_locus_tag="pgaptmp_004024"
     CDS             complement(5973..6668)
                     /gene="minC"
                     /locus_tag="ACHKNB_RS20120"
                     /old_locus_tag="pgaptmp_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HGU4197438.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7188..7556
                     /gene="ycgJ"
                     /locus_tag="ACHKNB_RS20125"
                     /old_locus_tag="pgaptmp_004025"
     CDS             7188..7556
                     /gene="ycgJ"
                     /locus_tag="ACHKNB_RS20125"
                     /old_locus_tag="pgaptmp_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HGU4197439.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(7676..8077)
                     /gene="pliG"
                     /locus_tag="ACHKNB_RS20130"
                     /old_locus_tag="pgaptmp_004026"
     CDS             complement(7676..8077)
                     /gene="pliG"
                     /locus_tag="ACHKNB_RS20130"
                     /old_locus_tag="pgaptmp_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HGU4197440.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8319..8612
                     /locus_tag="ACHKNB_RS20135"
                     /old_locus_tag="pgaptmp_004027"
     CDS             8319..8612
                     /locus_tag="ACHKNB_RS20135"
                     /old_locus_tag="pgaptmp_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HGU4197441.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8684..9343
                     /gene="ycgM"
                     /locus_tag="ACHKNB_RS20140"
                     /old_locus_tag="pgaptmp_004028"
     CDS             8684..9343
                     /gene="ycgM"
                     /locus_tag="ACHKNB_RS20140"
                     /old_locus_tag="pgaptmp_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HGU4197442.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            9435..9881
                     /gene="ycgN"
                     /locus_tag="ACHKNB_RS20145"
                     /old_locus_tag="pgaptmp_004029"
     CDS             9435..9881
                     /gene="ycgN"
                     /locus_tag="ACHKNB_RS20145"
                     /old_locus_tag="pgaptmp_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HGU4197443.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(10088..10981)
                     /gene="hlyE"
                     /locus_tag="ACHKNB_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /pseudo
     CDS             complement(10088..10981)
                     /gene="hlyE"
                     /locus_tag="ACHKNB_RS20150"
                     /old_locus_tag="pgaptmp_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         2902 a         2397 c         2606 g         3076 t
ORIGIN      
        1 atcaatgtca tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat
       61 tgtcgctgtt tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt
      121 ggtaatctga gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg
      181 ttaatctatg aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt
      241 gggtaatgca tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat
      301 aaaagatgga aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag
      361 ttacgggtat ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta
      421 acaacgggcg gtttatatcc ttctgacaat agtcctctaa gcacagcatt aacagctagc
      481 gcaatcgtaa gtgaatttgg cggtgctgtt attttgcagg gtaaaaacac gattgtcata
      541 acgggggatt actcaattgg tttgctaagt caaacgagtg gaaacctgaa tactgacacc
      601 ataataagag tcaactctga cggttcggtt accccctctt tttctgacgg agatgataca
      661 tttattgtta ctgcgggtaa tcacgcagtc ggcgttcttg cgtgtgcatc tcccggaagc
      721 gcgcgtgcgt gtgtatcttc tcttgatgaa gaaagtacta ccgatacagg aagtaatgaa
      781 aataatgcca tagcaaaact ggatatggca aaaggggaga tcacaaccca cggaacagaa
      841 agttatgccg cgtacgctaa tggcactgtc gtcaaggctg ttgacacgct tgattatacc
      901 aatgccagcg ttacattaac cgatgttgat atcaccacgc atggggacaa tgctcatgcc
      961 atcgctgccc gccaggggac agtttcattt aaccagggag aaatttatac aacgggtcct
     1021 gacgccgcga ctgctaaaat ttataatggc ggcacggtga cgctgaaaaa tacatctgca
     1081 gtcgcgcatc aggggtctgg aattgtgctg gagtcttcca taaatggtca ggaagcaacg
     1141 gtagatattt tatctggtag ctcactacgg tcagcaaatg aaatcctcta ccataaaaat
     1201 gagacgagta acgtgaccat tacggatagt gaggtatcat cggctgcaga tgtttttatc
     1261 aataatatta aaggtcattt gaccgtcgat gcaactaatt caaaaataac gggttcagct
     1321 aatatttcaa cagatgttaa cactcatacc tatctgtcgc tttcagataa tagtacctgg
     1381 gatattaaag ctgactcaac ggtgagcaac ctcacggttg ataatagtac ggtctatatt
     1441 tcccgggcag atggacggga tgtcgaacca acaagattaa caataactga aaattacgtt
     1501 ggtaataatg gcgtattgca tctcagaact gaattgggtg atgataattc agctacggat
     1561 aaagtcgtta ttaatggaaa tacctctgga acaacccgag ttaaagttac caatgcagga
     1621 ggcagcggag cttacacgtt aaatgggata gagattatca gcgttgaggg ggaatcaaat
     1681 ggggaattta ttaaggattc gaggattttc gccggtgcct acgaatattc attaacccga
     1741 ggtaataccg aagcgaccaa taaaaactgg tatctgacta acttccaggc aacgagcggc
     1801 ggtgaaacaa actccggagg aagttcagcg cctactgttg cgcctacccc cgtcctgcgc
     1861 ctcgaagctg gaagttacgt cgccaacctg gcagccgcta acactctttt tgttatgcgt
     1921 ctgaacgacc gtgcgggtga aatgcgctac atcgatcctg taactgaaca ggagcgttca
     1981 agccgacttt ggctacgtca aattggcggg cataatgcct ggcgtgacag caacggacag
     2041 ttgagaacga cctcgcatcg ctacgtctcg cagttagggg ccgatctgtt aaccggtggt
     2101 tttaccgaca gtgacagttg gcgtttggga gtgatggctg gttatgcccg cgactacaac
     2161 tcaactcatt ccagcgtgtc ggattatcgt tcgaaaggga gtgtcagagg ctattgcgca
     2221 gggctgtatg ccacctggtt tgccgatgac atcagtaaaa aaggcgcata cattgacgcc
     2281 tgggcgcaat atagctggtt taaaaactcg gtgaaagggg atgaattagc ctatgaatcc
     2341 tatagtgcga aaggcgcaac cgtctcgctg gaagcgggtt acggctttgc cctgaataaa
     2401 tcctttggtc tggaagcggc gaaatacacg tggatattcc agccacaggc acaggctatc
     2461 tggatgggcg tcgatcataa tgcgcacacc gaagccaatg gctcacgtat tgagaatgac
     2521 gcaaataaca acatccagac ccgacttggt ttccgcacct ttattcgtac tcaggagaaa
     2581 aacagcggtc cgcacggtga cgactttgaa ccttttgttg aaatgaactg gatccataac
     2641 agtaaagatt ttgctgtctc aatgaacggt gtgaaagtcg aacaagatgg ggcgagtaat
     2701 ttgggggaaa ttaaacttgg cgtaaatggc aacctgaatc cagcggccag cgtctggggg
     2761 aatgtgggcg tgcagctggg tgataatggc tacaatgaca ccgcagtgat ggtgggcctg
     2821 aaatataagt tctgatcccg ccgttagcta aaaaaccgcg tcgtattcat cgacgcggta
     2881 catatgaaat attatttttt gccgatagca cgcatggtgt catcaattgc cgtgatcaac
     2941 agcatttgcg ggtctttagc gcaaacctga ttcagttttt ctaccacttt ggcgctcagt
     3001 tccggagatt gctcaatttt taaatcacgg ctggcaacgc tggcattacc cattaccgca
     3061 acaatttctg caacctgtgc gctgtcagtt tttgccattt cgttggcttc tgcgcaagta
     3121 atataggttt ctgacggcaa accgttttta atattgtagt cctgcgccca ggtcattggt
     3181 gcgaaaacaa acaggcccgc cagtaaagca aattttttca tcatcattcc ttatttcatt
     3241 ttacccagaa ttgcaccacc cgtaccgcca atcacggcac ctttaatcgc cccttcgagg
     3301 ccattgccgg tcagaacgcc agtgacagca ccaacggcgg cacccacttt tgcaccttta
     3361 cgcgcatttt taccgtcgcg gcctttttct gttactgcac caacaccagc gccaacagct
     3421 gcacctttca gtacgccatt aacaccattg ccagtaagta aaccaacgcc tgcgcctagc
     3481 aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg gcgtggagca gaacaatgct
     3541 gagataagcc cgaaggcaag tatttttttc ttcaacttag atgtccggta ttaagtaagt
     3601 tgcacacaca ataatttcgt cttcaattaa gatctgctta actaaagaac gctcgctatt
     3661 attcagataa ttcaaaatga gcgtggctgt gatgatagga attatgtttt ttacgtgaat
     3721 gagaataatc ttaaatgagg aataactcat tgattgacaa tatttttatt caagaagtgt
     3781 cattgactgt taacgcaatg ttgtaaaggt aagataatct gatttatcaa tatcattgtg
     3841 tgatttttat gtgagcagaa gatattcatc agcaacgatt acattagtca ttttattttg
     3901 ccgacggcct cattgtcgaa agataagcgt acgacagtat tatctgaaaa gagtgatttt
     3961 ttatccaact acacttcagc gcactgcgtg taaaaaatgc ctctttctta tgcgggatat
     4021 catcatttca tcatgatgtc tttggtgagc ggtgaacaca atacacctgc gctgtctctt
     4081 caggatgaat cccctggtct ggtgcctgcg ggctgatgtt gcagcagagc ttaggtcact
     4141 tagacgctac tatcatttat ccaatggcat ggaatcgaaa tcagtcgata cccgcagtat
     4201 atatcgtgaa ctgggtgcaa cgctgagtta caacatgcgt ctggggaacg gtatggaaat
     4261 tgaaccctgc ctgaaggcgg ctgtgcgcaa agaatttgtc gatgataatc gggtgaaagt
     4321 gaatagtgac ggtaattttg tcaatgattt gtcgggcaga cgtggaatat accaggcagg
     4381 tattaaagcc tcattcagca gtacgttaag cgggcatttc ggggtggggt atagccatgg
     4441 tgccggtgtg gagtccccgt ggaacgcggt ggctggtgtg aactggtcgt tctgaccatc
     4501 aacgattaaa ctgcgcttcg gcgcagtttt cgtttacagg atgttgaaag agaaaattct
     4561 ggggcaaaaa aagcccgcca gttacggtgg gaaacctcat cctatgggag aacaatgaat
     4621 aatgaaattg cggggttatc atctcccagt atatccacac taacaacagg gttatttact
     4681 caaccaggca taaacatttt gttctgtgcg tgggaacagc cttaaggtgt aaagggggag
     4741 gtggaaatag caatgaggag tatcagcaag aatactcgcc gctttaccac aacgtggatg
     4801 agagggatga aaaactcaag gcagaaatga ctctgccttg aagataaatg cgtttttaca
     4861 gcgggcttat ttcagctctt ctgcttccgg taaggtcacg ttcagctcaa gaatagaaat
     4921 atcgccatct ttttgctcaa gctgtacggt taccatctca ggatcaattt gtacgtattt
     4981 acaaatgacc tcaagaatat ctttacgcaa ctgcggcaga taatgcggtt ctgcatcgct
     5041 gcgacggcgt tcagcaacaa taatctgcag ccgttctttt gcaatgttgg ctgtgttttt
     5101 cttccgcgag agaaagaaat cgagtaatgc cataacttat cctccgaaca agcgtttgag
     5161 gaagcctttc ttctcttctt caatgaagcg gaaaggacgt tcttctccca acagacgttc
     5221 tacggtatct gcgtaggctt tacccgcatc ggcgttaatg tcgagaatga ccggttcacc
     5281 ctggttagag gcgcgcaata ctgattgatc ctctgggatc acgccgacga gtttgatgcg
     5341 caggatctcc agcacatctt ccatgctcag catgtcacct ctgcttacgc ggcctgggtt
     5401 atagcgcgtt aacagcaggt gctctttaat aggctcttcg ccattttctg cgcggcgtga
     5461 tttcgacgcc agaatgccta aaatacggtc agagtcgcgt actgaggaga cttccgggtt
     5521 ggtggtaata atggcttcgt ctgcaaaata gagtgccatt aacgcaccgg tttcaatccc
     5581 tgccggggag tcacaaacga taaattcaaa atccatcgct ttcagatcat caagaacttt
     5641 ggcgacccct tcacgggtga gggcatcttt atcgcgtgtt tgcgatgccg gaagaatata
     5701 gagattttca gtacgcttat ctttaattaa cgcctgattt agcgttgcat cgccctgaat
     5761 gacgttgacg aaatcgtaaa cgacccggcg ttcacaaccc ataatcaggt cgagattacg
     5821 caggccgata tcaaaatcta tcacgacagt tttctttccc ttctgggcca aaccagtggc
     5881 gatggccgcg ctggaggttg tcttaccaac accccctttg cccgaagtaa caacaataat
     5941 gcgtgccata gaaattcctt gttaaaaagg gatcaattta acggttgaac ggtcaaagcg
     6001 ttttcgacta actgcagtcg cgccgctttg ccataaaatt ctgctgggat ttgatcactc
     6061 agccagtatt cacctgcgat ggacaccagt tccgccatca ggttcgtaca aaatatttgc
     6121 gtttcccggt caccacttgc ccctgcaagc gcacgaccgc gcatcatgcc atagacatga
     6181 atgttcccat cggcaatcaa ttcggcccca gcgctaacgt ggcttgtaac aatcagatca
     6241 cattgtggag cataaatacg ctgaccggaa cgcaccgggg tatctattaa acgcgttttt
     6301 gtgaccggcg ttgtattttg cgctggagcc tgtggtgcgg gagctggacg cggcgctttt
     6361 tcctttcctt ccgtcaggat aggcagcccc atcttttcaa tttcggcttt aagttgcgca
     6421 tctttgcagc cacttacgcc aataacccgc aaaccggttg ccgaaaccgc cttatgcatc
     6481 gctgaccagt ttaccgggtc ttccagtgca ctgacgttga gtacaacggg ggcatgtttt
     6541 aaaaatgcgg gggcctgagc gattttgtct tccagcgcct gatggataac cttaggttct
     6601 gcctcatgca gatgaaccac agataaagtg aagctactgc ctttaagctc gattggcgtg
     6661 tttgacatcc tggccttact caattagcta ttaatcatcg ccagcgcgcg atgatgttcc
     6721 gaagactata aggcatgtta tagtctggat tatattgagg caagtcaccc tcccatttat
     6781 tcagagtaaa agtctattct gtgataaatg gcgctgattc atagcttaaa aaataccctt
     6841 gtcaatcaac ccattgccgt cgtacttttg attgttctta tttacgcttc ctttttccgc
     6901 accctaatcg tgacagcatc tccagacctg tttaaatatc cagtattgag gcaagtgtat
     6961 tttttcacca cgccaggctt tcttaacttt ccattttttg cggcatcatt aaagaacaga
     7021 tacccgttat cttaatatcc gccacagaat tgctctatca tagaaaccgc agaagacgtc
     7081 tgatacttct gtcaggtcat cgtaactcgt ccaggtcagc ggttttgatt acggattgat
     7141 gatgaaatga gcataagtgt tgtacggttg gattatcagg atgtgctatg aatatgatgc
     7201 gtatttttta tatcggattg tcaggtgtgg ggatgatgtt ctcatcaatg gcgtctggac
     7261 atgacgctgg cggacttcaa tctccggcgt gcggcgttgt ttgcgacccg tatatttgtg
     7321 tgaactcaga tggcatttcc ccagagttaa caaggaaata tctcggcgaa aaagccgctg
     7381 aaaacttaca atcattacaa ggctacgatc ccagcgaatt tacattcgct aacggtgtat
     7441 tttgcgatgt taaagaaaaa ttatgtcgtg atgatcgtta ttttggtgtg gatggtaagc
     7501 ggagtggaaa aatcaatcaa atcaccacaa agatgttatt tatgtgtcgt gagtgatgcc
     7561 ttgtttacat tatgttctat tcggcattgt ctgctattcg tcattaacct cttgagcgac
     7621 agagaggagt ggggcgttaa cgtttatgac ggggagagtc cccgtcggtt gacatttact
     7681 taatttgaat atcgacgtta tattttttag ttttgttttt acgggcgtcg tttcgggttt
     7741 gcagcactcg cagctcatat ttgccagaag cagggagtga gtattgtccg tgactatcga
     7801 gttctggtga atatctggat aaatcaactg aatcatcgat tcctggcccg aagaggtaag
     7861 tatcagcgcc ttcattagag atactcacat gcactttctg accttttttg gcatagaagg
     7921 tatatgtatc gtaatcgtat ccctttattt cgccggagta ctgagcgctg ctatggcctt
     7981 tgctgaactc aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa
     8041 gcagcaatac agccttactg atgctcttga ttttcattgg tagtctcctg ttcagaatga
     8101 gtacgatgaa ctgttataat ataacaatcc cttacgggag aattccagtg gcttttctgc
     8161 gttcggtaaa atcccggcgc ggcatttttt gccgggggga ttacttataa aggggcatgg
     8221 actcaacaaa ccgtagaaat ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt
     8281 agcttatgcc aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg atttatcgaa
     8341 gcagcaagcg tgaccagacc tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc
     8401 ctgaggaact gatgaaaggt tttggtcagc ctcagttagc gatgattctg ccgttggatg
     8461 ggcggaagaa actggtcaat gctgatattg aaaaggttaa actggcatta accgagcaag
     8521 gttactattt gcagttaccg ccaccacccg aagatttgct gaagcaacat ctttccgtca
     8581 tggggcagaa aacagacgac actaacaaat aaccgatatc cggcggtggc attatctttg
     8641 tcggcgcggg ttttcatatc cacgataagg tgaggggaac gttatgtatc aacatcacaa
     8701 ctggcaaggt gcgctgctgg attatccggt gagtaaagta gtctgtgttg gcagtaacta
     8761 tgccaaacat attaaagaga tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa
     8821 accagaaacg gcactgtgcg atctgcggca gccattggcg atcccatccg atttcggttc
     8881 agttcatcat gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc aggctacgga
     8941 agagcatgtc cgcaaagcca ttgccggtta tggcgtggcg ctcgatctga cattgcgtga
     9001 tgttcaggga aaaatgaaga aagccgggca gccgtgggaa aaggctaaag cgtttgataa
     9061 ctcttgtccg ctttccgggt ttattcccgc ggcggaattt accggcgacc cgcaaaatac
     9121 aacgctgggc ctgagcgtaa acggcgagca acgtcagcaa ggtacgactg cggacatgat
     9181 ccataaaatt gttccgctga tcgcttatat gagcaagttt tttaccctca aggccggtga
     9241 cgttgtgctg acaggcacgc ctgatggcgt cggcccgttg caaagcggtg atgagctgac
     9301 agtcactttc gatgggcatt ctttggcaac tcgcgttttg taatactttt tgccgcctga
     9361 aagcggcggc aaaacttgca tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa
     9421 tggcggaaca cctgatgagc gatgtacctt tctggcaaag taaaaccctg gacgaaatga
     9481 gcgatgcgga atgggagtcg ttatgtgatg gttgcggtca gtgttgcctg cataaactga
     9541 tggatgaaga caccgacgaa atctacttca ctaacgtcgc ctgtcgccag ctcaatatta
     9601 aaacctgtca atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat
     9661 taacccgtga aaatctgcca acattcgaat ggctgccaat gacctgcgct tatcgtttgc
     9721 tggcggaagg taaagattta cctgcgtggc atccgctact tactggttcg aaagcggcaa
     9781 tgcatggtga acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact
     9841 ggcaggatca tatcttaaat aaacctgact gggcgcagtg ataaatttaa tagatgttga
     9901 tgggggcttt cttaatatac gtctggatgc tcgccatttc gtagccctgt ttttgaaagc
     9961 gctctgtcat aatgagagtt acccgatacc ttattatcaa caggtaactc tcaatcttta
    10021 aaaaacgtct gtgtttttaa atgtgataaa ccttatacct tgagtacatg gagagaataa
    10081 tcgcttatca gacttcaggt acctcaaaga gtgtcttttt accgtgtctt ttctgatact
    10141 cattacaggt gttaatcatt ttttgccgct tcttttagca aagaaagcat taaatcatca
    10201 taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat ggcggctatt
    10261 tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt aaccgtgtta
    10321 gacagggtgg taaagaaatt ctgcacagat tttaacttgt tcttcaattc tggaatcagt
    10381 ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaatccaaa tggaccggcg
    10441 acgacaccgg ctgcggcacc ggcatacgct tccttcctga ttttatctac ctgtgactgg
    10501 aaaagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
    10561 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
    10621 tcgtgatgcc gtcatccagt accttaatga gaatgtcttt ctgggcggat gctttcttct
    10681 cattgtactc atcaaataga aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
    10741 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
    10801 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
    10861 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
    10921 gatctaatgc tccatctgca gtttcgattg cgtttttaac tacttctacc gttttatctg
    10981 c
//
LOCUS       DAZFVO020000157        10964 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFVO020000157 DAZFVO020000000
VERSION     DAZFVO020000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.158"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..832
                     /gene="kgtP"
                     /locus_tag="ACHKNB_RS20155"
                     /old_locus_tag="pgaptmp_004031"
     CDS             <1..832
                     /gene="kgtP"
                     /locus_tag="ACHKNB_RS20155"
                     /old_locus_tag="pgaptmp_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4197444.1"
                     /translation="RKGFYASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIP
                     FALGAVLAVVALWLRRQLDETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLC
                     FYTFTTYMQKYLVNTAGMHANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFG
                     SLAAIFTVPILSALQNVSSPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGV
                     GLSYAVANAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL
                     "
     gene            complement(829..1152)
                     /gene="yfiM"
                     /locus_tag="ACHKNB_RS20160"
                     /old_locus_tag="pgaptmp_004032"
     CDS             complement(829..1152)
                     /gene="yfiM"
                     /locus_tag="ACHKNB_RS20160"
                     /old_locus_tag="pgaptmp_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HGU4197445.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1198..2553)
                     /gene="pssA"
                     /locus_tag="ACHKNB_RS20165"
                     /old_locus_tag="pgaptmp_004033"
     CDS             complement(1198..2553)
                     /gene="pssA"
                     /locus_tag="ACHKNB_RS20165"
                     /old_locus_tag="pgaptmp_004033"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HGU4197446.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(2667..5327)
                     /gene="pat"
                     /locus_tag="ACHKNB_RS20170"
                     /old_locus_tag="pgaptmp_004034"
     CDS             complement(2667..5327)
                     /gene="pat"
                     /locus_tag="ACHKNB_RS20170"
                     /old_locus_tag="pgaptmp_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HGU4197447.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(5359..6057)
                     /gene="tapT"
                     /locus_tag="ACHKNB_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(5359..6057)
                     /gene="tapT"
                     /locus_tag="ACHKNB_RS20175"
                     /old_locus_tag="pgaptmp_004035"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HGU4197448.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6126..6545)
                     /gene="trxC"
                     /locus_tag="ACHKNB_RS20180"
                     /old_locus_tag="pgaptmp_004036"
     CDS             complement(6126..6545)
                     /gene="trxC"
                     /locus_tag="ACHKNB_RS20180"
                     /old_locus_tag="pgaptmp_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HGU4197449.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            6752..7789
                     /gene="yfiF"
                     /locus_tag="ACHKNB_RS20185"
                     /old_locus_tag="pgaptmp_004037"
     CDS             6752..7789
                     /gene="yfiF"
                     /locus_tag="ACHKNB_RS20185"
                     /old_locus_tag="pgaptmp_004037"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HGU4197450.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(7837..8526)
                     /gene="ung"
                     /locus_tag="ACHKNB_RS20190"
                     /old_locus_tag="pgaptmp_004038"
     CDS             complement(7837..8526)
                     /gene="ung"
                     /locus_tag="ACHKNB_RS20190"
                     /old_locus_tag="pgaptmp_004038"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGU4197451.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            8831..9214
                     /gene="grcA"
                     /locus_tag="ACHKNB_RS20195"
                     /old_locus_tag="pgaptmp_004039"
     CDS             8831..9214
                     /gene="grcA"
                     /locus_tag="ACHKNB_RS20195"
                     /old_locus_tag="pgaptmp_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HGU4197452.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9270..9857)
                     /gene="eamB"
                     /locus_tag="ACHKNB_RS20200"
                     /old_locus_tag="pgaptmp_004040"
     CDS             complement(9270..9857)
                     /gene="eamB"
                     /locus_tag="ACHKNB_RS20200"
                     /old_locus_tag="pgaptmp_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HGU4197453.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            9960..10841
                     /gene="yfiE"
                     /locus_tag="ACHKNB_RS20205"
                     /old_locus_tag="pgaptmp_004041"
     CDS             9960..10841
                     /gene="yfiE"
                     /locus_tag="ACHKNB_RS20205"
                     /old_locus_tag="pgaptmp_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4197454.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2722 a         2744 c         2863 g         2635 t
ORIGIN      
        1 gcgcaaaggt ttttacgcat catttcagta tgtgacgttg atcggcggac aactgctagc
       61 cctactggtt gtcgtggttt tacaacacac catggaagac gctgcactca gagagtgggg
      121 atggcgtatt cctttcgcgt taggagctgt gttagctgtt gtggcgttgt ggttacgtcg
      181 tcaattagat gaaacttcgc aacaagaaac gcgcgcttta aaagaagctg gatctctgaa
      241 aggattatgg cgcaatcgcc gtgcattcat catggttctc ggttttaccg ctgcgggctc
      301 cctttgtttc tataccttca ccacttatat gcagaagtat ctggtaaata ctgcgggaat
      361 gcatgccaac gtggcgagtg gcattatgac tgccgcattg tttgtattca tgcttattca
      421 accactcatt ggcgcgctgt cggataagat tggtcgccgt acctcaatgt tatgtttcgg
      481 ttcgctggca gccattttta ccgttcctat tctctcagca ttgcaaaacg tttcctcgcc
      541 ttatgccgct tttggtctgg tgatgtgtgc cctgctgata gtgagttttt atacatcaat
      601 cagtggaata ctgaaggctg agatgttccc ggcacaggtt cgcgcattag gcgttggcct
      661 gtcatatgcg gtcgctaatg ctatatttgg tggttcggcg gagtacgtag cgttgtcgct
      721 gaaatcaata ggaatggaaa cagccttctt ctggtatgtg accttgatgg ccgtggtggc
      781 gtttctggtt tctttgatgc tacatcgcaa agggaagggg atgcgtcttt agtgacgggt
      841 cagttgccag acggtatagc cggtgcttgc accggcgaca tcccaggcca aatccttcca
      901 gctccagccg ctcccttcgg ggcggctatc ccaaagctct tttgacgccc ccaaactgac
      961 agagaacatc aatccaaaca tggcactgcg atcccggctc atcccctgat gctgtgaata
     1021 ttcatttccg gcggcggaaa gcatcgccga ggcgataaag tgttgagctt tatcctgccc
     1081 gctccagcta tcgttagcca tatggctgca tccagaaagt aacaatagcg aacagacaaa
     1141 aagaatacgc aaaacgtggc ctccaaaaaa caaaccccgt acaggacggg gttgtgatta
     1201 caggatgcgg ctaattaatc ggtcgatgcg gatacggcgc aaacggcgga tgagtttacg
     1261 aaccttcacc ggataatcgg caatactttg cagatcgcga tagtgcttaa cgatggtggt
     1321 atgctcgcgg atcagctcca gttctttctc tcgctgcggc gcaagctcca gttgtggatc
     1381 gtggatcaaa atggcgtttt ccagatccag acgccaggcg cgcgggttca ggttattacc
     1441 ggtgatcaac atccacttat catcaaccca catccctttt aggtgatagg tgttgtcgtc
     1501 atctttccat aaccgaacca ctagctggtc agtattgacg taatactgca aacggctcag
     1561 gaaacgacgc agattgatct catagagata aggcaatgcg ccaattatct tgaaaggttc
     1621 atcttccgga atgtagaagt cgttcgcggt tttatcacca acaataattt cgaccttttt
     1681 cccttcgcgc agcaactgga taatattgcg cacaaggatt gctggcaggt tgaagtatgg
     1741 cgtacagatg gttagtttct gctcggcaca aggcataaga tggaaaatgg tcttgttcaa
     1801 cagactcgat ttccccagcc ccactagcgg cgttacggaa agctgatcgt tgtcggcatc
     1861 gccctggaaa tgataagcgg catcacgcag ctcctggcgg aacagacgaa tatcgttctt
     1921 gatttccggg ctttttggcc gattaacatc atccagacga ttaacgccgc ggccattcat
     1981 aatattctgt gtaacccatt caaacataat gtctgacatc ttacggttac ggataagatg
     2041 ataacggtcg tagcgatatt tatcgtgctg atgcaggtaa acatcgttca ggctggcacc
     2101 gctataaagt acgctatcgt cgatgataaa gcctttaaag tgcagaacac caagggcttc
     2161 acgggtattg attggaacgc cataaaccgg aacatctacg cccggatttt cctgcgccat
     2221 gcggcagtac cagtcagcgt tagtgttaga tgccgcagcg ccaatgcgtc cacgttgtgc
     2281 acgatgccag tcgaccagca cccgcacatc cagttccgga cgctgccttt tagcttcata
     2341 caacgcgttc agaatgcctt tgccaccgtc atcctgttcg agatacaggg cgacaatgca
     2401 aatgcgctgc ttcgcgctgg ctattttttc cagcagcgtc tcccggaagt cggcgggagc
     2461 gtaaaagaaa tcgacatcat caactgattg agaaatcttg ggtagttggg caaggtgttg
     2521 ttgatgttta ttacgcttaa attttgacaa catcacagtg catttcttct ctgttcattg
     2581 aagggtcctc tgtgcaatgc agacgacata agcgggcaat aataacacca gtcccgatta
     2641 agtggtcaac atttccagta ccttactcat gattcctcgc gttgggcaag atttagcgta
     2701 agcccaacga tcccctcttc gagctggata tcaacgttaa acccgagctt gcgggctagc
     2761 gccaccatgc cacgattgtt tggcatcgta ataccattca gacgttgtag tccgtgatct
     2821 cgcgtatagg taatcaactt ttccattaag cgtcgaccta agcctaaccc tttgagatcc
     2881 gagcgaacca gcacagcaaa ttcggcatcg atgttatcag gatcggaaat cgcacgcgtg
     2941 acgccgagga tctcttccgt ttgatcaata cgtcgtaccg ctacaaacgc catttcccga
     3001 tcgtagtcga tctgtgtcat gttggctaaa tcttcatggg taaattcgtt gatctcgcta
     3061 aagtagcggt aataaagatc ttctttggtg actcgcgaaa tgaattgctg aagttgtggc
     3121 tcatcttctg gcaaaatcgg gcggaacaag cagcgttcac cgtttttcaa ttctacccat
     3181 tcttccagct gatgcggata agggcgcact gccagccgac tctcgttatc gccttcaaac
     3241 ggcgagatat ccagcgtgac atccagcgcg gtaaattcac tgccagaagc cagcaaagga
     3301 tgaatatcca gacgctgaat ttccgggcaa tcgacaatca agttggaaac ctgcaccaga
     3361 agctggctca agcctgcaac atccaatggg cgtagcgcac tgcgtgcacg aatcttttta
     3421 cttttgatcc cctgaataac cagatagcgg gccaggttca tgttcagcgg cggtaacgcg
     3481 acgacggctt gatcttcagg acgccactcc acaccgcctt cgcccagcat gatcaacggc
     3541 ccgaaaaccg gatcgtgctc aaccacaacc cgcaactcct gagcaccagc acggttagcc
     3601 atactttgca ccaacaggcc gtggacccgt gcctgcggcc aggccatttt tacgcgatcg
     3661 aaaatggcgt ttgccgcttg ctggacttca ttggctgtac gcaggtagag catgacgccc
     3721 tgaacttccg atttatgtgg aatatccggc gaacgcaatt tcagcgccac cggataacca
     3781 atctgttcgg caatatgcac cgcttcggtg ctatcgctgg caatccaggt ggggagcgtg
     3841 ttcatgccat atgcttgcaa gataggctga acttcatggg tatcgagtga cgttgctcct
     3901 tcggcaatcg cctgttgcaa cagaagatgc gcttctgcgg tattggaggt cagattgctg
     3961 ggcaacgccg gcgtttcgcg tagttgcttc tgattacgcc ggtactccac catatgcata
     4021 aaagcagtga tggttccttc cggggtacgg taggtcggca gcccggcttc gctgaataaa
     4081 cgtcgtgcct cttgcgagga gtgctcgccg caccagttcg tcagcaaaga gacatatttg
     4141 ctgcggggat gatgctttac cgcttcaatt aattcttgcg cgctttctgt tgcgggagcc
     4201 gctgcgctgg gtgaatgaat aaccatcagc gcatcaaaat cctggctgtg gagcagaatg
     4261 tccagcgttt tgacataatg ctcgctgctg gcgtcatcgc gcagatcgag cgggttagag
     4321 attgccacat gtccaggcag tgcatcgcgc agtttctggc aggtttcttc gcttagcgtt
     4381 gccagcttgc cattgtgtga ccataaggca tccagcgcca gcgcggcagg cgcagcaccg
     4441 ttgctgataa tcatcaaccg gtcgccacgc agcgggcgca tatggctaag ggtttccacc
     4501 gccgaaaaca gctcgtgggt gtcctgtacc cgcaacaaac cggcacgctg aatagccgca
     4561 tcccacgccg gatccattcc tgccgtggtg ttcaacagac gttgagccgc cgggctacgt
     4621 ccgcttttaa tcaccagaat cggtttatta cgcgaggcac tacgggccgc cgaaacaaag
     4681 cgtcgcgcgt cgcttaactg ttcgagatag agcaggatgg cgctggtttt actgtcgcgc
     4741 gcaagatagt cgagcaattc atcaacgtcg atatccaggc tgtcgccgag cgcaataaag
     4801 taggaaaagc ccatctcacg ctgttgcgcc cagtcgagga tggtgttgga gacggcagcc
     4861 gattgtgaaa taaacgccag cttgccgcgt ttaatcggca caggcgaaaa gctggcattc
     4921 agaccttgcc agggagccag taaacccaga ctgtttggtc caagcaggcg catgttatgg
     4981 cgcagggcgc aggcgcggag atcttcgtgt tgcgatgccg gggcggaaag aataatgcag
     5041 gttttacagc ctttctcgcc gagctcttcc agaagagcaa gattacggct ggcattggta
     5101 cataaaaccg caaggtcggg tgtaaagggc aagctggcaa tatccggcca ggccaacaca
     5161 cccaacaccg ctttccaggc tggcgtcacc gggagtaccg gtccgttaaa gcctcccgcc
     5221 agcaggttac gcatcatcag gtaacctgcg cgattgggtt tcatcgacgc gccaattacc
     5281 gctatcgatt ttggtcgcag tagtgcttcc agtcctcgct gactcatacc ggtctcccgc
     5341 agaagtgacc gaatgatttt aaacgctttc taactgttct gctgtgatgc tacccagatg
     5401 ttgcgttttt cctgccagat agcgtgtttt aaagcgggta aaatgctcgc ctaaccctgc
     5461 cgccgccccg gtatcgccgg ccatatctaa cagtgcgatg gctacctcgg cagtacaata
     5521 ttggcctgca gcctgggctt cacgcaggcg ataggcagaa agccgggaaa gatcgacgga
     5581 aatgacggga agattatcca gatacggact tttacgaaac atcttgcgag cttccggcca
     5641 ggtaccatcg agcatgataa acagcggtgg cttaccggca ggtggtgtga agatcacttc
     5701 ccgttgctca tcagcatacg aggcgggaaa gaccaccatt ggctgataat ccgggttttg
     5761 taccagatcc agcaaatcct gcgagggttc ggtacgcgac cattgaaacg caacggtatc
     5821 aggcaaaata tcagcaatca gacgcccggt attactgggc ttcattggct cggtgtcgaa
     5881 catcagcaaa cagaagcgac tttttgcttg tgctggggta attgtcgaac agagacataa
     5941 tttctctggc aaaagacagc gttggcagcg acgaacgcga ttaccgcggg caagaaaagg
     6001 acgtgttgcg cgcgcaatac gctcggcgcg taactggaga acagcgtttt cggtcataag
     6061 agagcgtcga aaaaacgcca ttgtcgcaga ggagaaaacg gggcacaaga tgcgccccgg
     6121 taagattaaa gagattcgtt cagccagcta tcgaacggcg ctttcggtac tgcgccatta
     6181 agcatgtcga caacctgacc gtttttgaaa atcatgatcg tcggtatact acgaattcca
     6241 aagcgactgc tcaattcacg ttcagcttcg gtattcactt tcacaaagcg cactttaccg
     6301 ctacgctctt gcgcgacatc ttcaaaaatt ggtgcgaaat tacggcaggg gccgcaccac
     6361 ggtgcccaga agtcgatcac cacaggtaga tcatccttca gcaatttgtc gagtgtttca
     6421 ccggtcgcat taatcacctc tccgtcaaac aagtcgtgac cgcagcgtcc gcattttgcc
     6481 gcatcttcga tccgatcgtc gggaatgcga ttgatggcct gacaatgggt acaaacggta
     6541 ttcataacta acctcgggat gagtaaggag acttatcggc aacgacgccg gtatgtttct
     6601 aatatgttac atattatcgg ggacattgtt ccagacaaca atctgtttta ttcaataggt
     6661 atgatagtcg caggctttgg tacgaaatca tggcgaaccg cgcggacttc gggtaatctg
     6721 cgcgcttcgc gcagcgctgg tggagaaaag catgaacgat gaaatgaaag gtaaaagcgg
     6781 caaggtcaaa gtgatgtatg tccgcagtga tgatgattct gataaacgta cccacaaccc
     6841 gcgtaccggg aaagggggcg ggcgtccagg aaaatctcgt gctgacggtg gccgtcgccc
     6901 cgcccgcgat gacaaacaga gtcagccccg tgaccgcaag tgggaagatt cgccgtggcg
     6961 cacggtttcc cgcgcgccgg gtgatgagac gccggaaaag gccgatcacg gtggcatcag
     7021 tggtaaaagt tttattgatc cggaagtgtt gcgtcgtcag cgtgcggaag aaacccgcgt
     7081 ctacggcgaa aatgcatgtc aggcactttt ccagagccgt ccggaagcga ttgttcgcgc
     7141 ctggtttatc cagagtgtaa cgccgcgttt taaagaagcc ttgcgctgga tggcagcaaa
     7201 ccgcaaagcg taccatgtgg tggatgaagc ggaattgaca aaagcgtcag gcacggaaca
     7261 tcacggcggc gtttgcttcc tgatcaaaaa gcgtaatggc acaaccgtgc agcagtgggt
     7321 aagccaggca ggcgcgcagg attgtgtact ggcgctggaa aacgaatcta acccgcataa
     7381 cctgggtggc atgatgcgca gctgcgcgca cttcggcgtg aaaggcgttg tggtgcagga
     7441 tgcggcactg ctggagtcgg gggcggctat ccgtaccgca gaaggcggcg cagagcacgt
     7501 tcagccgatt actggcgaca acattgttaa cgtgttggat gatttccgtc aggcaggtta
     7561 caccgtagtg accacttcca gcgagcaggg gaaaccgctg ttcaaaacca gtctgccagc
     7621 gaaaatggta ctggtgctgg gtcaggaata tgaagggtta ccggatgccg cacgcgatcc
     7681 gaacgatctg cgcgtgaaga ttgacggtac gggcaacgtt gccgggctga atatctctgt
     7741 tgcaaccggc gttttgcttg gtgaatggtg gcgtcagaat aaagcctgat aaatcagccg
     7801 ggtggcaact ctgccatccg gcattttacc gcaaatttac tcactctctg ccggtaatac
     7861 tggcatccag tcaatcggcg tctcgccacg ttgttccagc cactgatttg ccagcacaaa
     7921 atggttgcag ccaaagaatc cacgatgcgc cgaaagcggc gacggatgcg gtgctttcag
     7981 tacatgatgg cgttgcttat ctataatcgc ccctttcttt tgcgcatgtg atccccacaa
     8041 caaaaacacc acgccttcgc ggtgctggtt aatcaggctg atgactttat cagtaaacgt
     8101 ctcccaaccg aggctggcgt gggaatgcgc ctgacctgcg cgaaccgtca acacagtatt
     8161 gagcagcaga acgccctgac gcgcccagct ttcgagatag ccatgattag ggcgggtgaa
     8221 gcccggaata gtattttcca gctctttata catattcaat aatgacggag gaatggcaat
     8281 gccgggacga acggaaaatg ccagaccatg cgcctgtccc ggtccgtgat aaggatcctg
     8341 gccgagaatc accactttaa cgtcacccaa ctcggtaaag cggaacgcgt taaagacatc
     8401 tttttgtggt ggatagatag tgacgccgga ctgccgctcg ctggcgacgg tctgaagggt
     8461 attaagaaaa taaggttgtt gcttctcttc agccagcacg tcatgccagg ttaattcgtt
     8521 agccatctcg ctctcctgcg aatcttcaat ccgcctagct taactgcttc tttctacaga
     8581 acaaaatcat gctcattttt tagacttaaa cgagtgcaaa aaaatgtaaa ttggtaaaat
     8641 ttcacacccg cataagttga tgtaaaacaa taaaatccat ttacgaccac aatcgatgtg
     8701 tggtttttat tgatttaaat caaagaatca agggtggttg aggagtatat atacactcaa
     8761 gcaacaatgg ttttaccaat tggccgcgac aggctgaaca aatcaaataa ttttgccggg
     8821 gaggcatcac atgattacag gtatccagat tactaaagcc gctaacgacg atctgctgaa
     8881 ctctttctgg ctgctggaca gcgaaaaagg cgaagcacgt tgcatcgttg caaaagcagg
     8941 ttatgcagaa gatgaagtgg ttgcagtaag caaactgggt gacattgaat accgtgaagt
     9001 tccagtagaa gtgaaaccag aagttcgcgt tgaaggtggt caacacctga acgttaacgt
     9061 tctgcgtcgt gaaactctgg aagatgcagt taagcatccg gaaaaatatc cgcagctgac
     9121 catccgtgta tccggttatg cagttcgctt taactctctg actccggaac agcagcgcga
     9181 cgttatcgct cgtaccttta ctgaaagcct gtaaggctta agtagcaagt tactaagcgg
     9241 aagagggcga cctcttccgc tttttttcgt taatagaaaa tgcgtaccgc gcaatagacc
     9301 agcaacaggg caagcacgat atttaactgg cgaccatact ggcgaaacaa tcgctgaaac
     9361 agatgccccg ccagcgccca gcacacattg ccaaacgtcc caatcatcgc cagcaaaacg
     9421 ctgacgccaa ctacccagct tagcgcctgt gtttgcggca gaacaaacgt cgacagtgcc
     9481 gtaacaccgt acaaaatgat tttgacgttc acaaactgca aagcaaagct ggcccaaaag
     9541 ctgattggtt ttgcctgaag tccgtcttcc tttgttgggc tggtggcgat tttccacgcc
     9601 agccagacaa tatatgccgc ccccgcccaa ctcaaaaggt gtaccgctgc cgggtcaatc
     9661 actgccagtg aaaatgaaat gcccgcacac agtaacatca caatcaaaaa tcccagactc
     9721 atccctgcca gcacgcgggt actttgacga aatccatgcg acgtagcaga gctaagggcg
     9781 agaatattgt tcggtcctgg cgtcatagcg gtaatcaggg tgtaagtcca aaaagcactt
     9841 aaaagggtcg gtgtcacttg ttatctcctc tgttttggta gacagtaccc gacacggaac
     9901 atttaaaaaa atgaatgttt ttatattcta gattcgatat tttcgatagg tttggggtta
     9961 tggatctgcg ccgttttatt acgcttaaaa ccgtggtgga agagggttcc tttttgcgag
    10021 cttcgcaaaa attgtgctgt acacaatcga cggtgacttt tcatattcag cagcttgagc
    10081 aggagttttc agtccagtta tttgagaaaa ttggccgacg gatgtgcctt acccgcgaag
    10141 gaaaaaagtt gctgccgcac atttatgaac ttacccgagt gatggatacg cttcgtgagg
    10201 ctgccaaaaa ggagtcagat ccggacgggg aactacgcgt tgtttcgggc gaaacgttgc
    10261 tctcttaccg aatgccgcag gtgttgcagc gttttcggca acgcgcacca aaagtgcgtt
    10321 tatcgttgca gtcgctgaac tgttacgtga tccgtgatgc gctactgaat gatgaggccg
    10381 atgtcggcgt cttttatcgc gtagggaatg atgatgccct gaaccgacga gagttggggg
    10441 aacaatcgct ggtgctggtg gcttcaccgc aaattgcaga tgtcgatttt actgagccgg
    10501 gaagacataa cgcctgtagc tttattatca acgaaccgca atgtgtcttc cggcagatat
    10561 ttgagagcac gctgcgtcag cggcggatca cggtggaaaa caccattgag ctgttaagca
    10621 ttgaaagtat caaacgttgt gtcgcggcga atatcggtgt cagttacttg ccccgctttg
    10681 cggtggaaaa agagcttgag agcggagaat taattgaatt gccttttggc gaacagtcgc
    10741 agaccattac ggccatgtgc gcacaccatg ctggaaaagc ggttagcccg gcgatgcaca
    10801 cttttattca gtgtgttgaa gagagttttg tggctggata aaaataatgt cgggattaaa
    10861 cccggcattt aacttgtcga caaagtgcgc tttattcatg ccggatgcgg cgtgaacgcc
    10921 ttatccggcc tacaagatcg tgcaaattca atatattgca tgag
//
LOCUS       DAZFVO020000158        10935 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFVO020000158 DAZFVO020000000
VERSION     DAZFVO020000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.159"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..493)
                     /gene="yobD"
                     /locus_tag="ACHKNB_RS20210"
                     /old_locus_tag="pgaptmp_004042"
     CDS             complement(35..493)
                     /gene="yobD"
                     /locus_tag="ACHKNB_RS20210"
                     /old_locus_tag="pgaptmp_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HGU4197455.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(548..1399)
                     /gene="manZ"
                     /locus_tag="ACHKNB_RS20215"
                     /old_locus_tag="pgaptmp_004043"
     CDS             complement(548..1399)
                     /gene="manZ"
                     /locus_tag="ACHKNB_RS20215"
                     /old_locus_tag="pgaptmp_004043"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HGU4197456.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1412..2212)
                     /gene="manY"
                     /locus_tag="ACHKNB_RS20220"
                     /old_locus_tag="pgaptmp_004044"
     CDS             complement(1412..2212)
                     /gene="manY"
                     /locus_tag="ACHKNB_RS20220"
                     /old_locus_tag="pgaptmp_004044"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HGU4197457.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2275..3246)
                     /gene="manX"
                     /locus_tag="ACHKNB_RS20225"
                     /old_locus_tag="pgaptmp_004045"
     CDS             complement(2275..3246)
                     /gene="manX"
                     /locus_tag="ACHKNB_RS20225"
                     /old_locus_tag="pgaptmp_004045"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HGU4197458.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3563..3721
                     /gene="yoaL"
                     /locus_tag="ACHKNB_RS20230"
                     /old_locus_tag="pgaptmp_004046"
     CDS             3563..3721
                     /gene="yoaL"
                     /locus_tag="ACHKNB_RS20230"
                     /old_locus_tag="pgaptmp_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HGU4197459.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3709..5265
                     /gene="yoaE"
                     /locus_tag="ACHKNB_RS20235"
                     /old_locus_tag="pgaptmp_004047"
     CDS             3709..5265
                     /gene="yoaE"
                     /locus_tag="ACHKNB_RS20235"
                     /old_locus_tag="pgaptmp_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HGU4197460.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(5269..6867)
                     /gene="pdeD"
                     /locus_tag="ACHKNB_RS20240"
                     /old_locus_tag="pgaptmp_004048"
     CDS             complement(5269..6867)
                     /gene="pdeD"
                     /locus_tag="ACHKNB_RS20240"
                     /old_locus_tag="pgaptmp_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HGU4197461.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6998..8362)
                     /gene="sdaA"
                     /locus_tag="ACHKNB_RS20245"
                     /old_locus_tag="pgaptmp_004049"
     CDS             complement(6998..8362)
                     /gene="sdaA"
                     /locus_tag="ACHKNB_RS20245"
                     /old_locus_tag="pgaptmp_004049"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HGU4197462.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8546..9124)
                     /gene="nudL"
                     /locus_tag="ACHKNB_RS20250"
                     /old_locus_tag="pgaptmp_004050"
     CDS             complement(8546..9124)
                     /gene="nudL"
                     /locus_tag="ACHKNB_RS20250"
                     /old_locus_tag="pgaptmp_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HGU4197463.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(9128..10489)
                     /gene="pabB"
                     /locus_tag="ACHKNB_RS20255"
                     /old_locus_tag="pgaptmp_004051"
     CDS             complement(9128..10489)
                     /gene="pabB"
                     /locus_tag="ACHKNB_RS20255"
                     /old_locus_tag="pgaptmp_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HGU4197464.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKHTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10563..10742
                     /gene="yoaH"
                     /locus_tag="ACHKNB_RS20260"
                     /old_locus_tag="pgaptmp_004052"
     CDS             10563..10742
                     /gene="yoaH"
                     /locus_tag="ACHKNB_RS20260"
                     /old_locus_tag="pgaptmp_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HGU4197465.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(10862..>10935)
                     /locus_tag="ACHKNB_RS20265"
                     /old_locus_tag="pgaptmp_004053"
     CDS             complement(10862..>10935)
                     /locus_tag="ACHKNB_RS20265"
                     /old_locus_tag="pgaptmp_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 domain-containing protein"
                     /protein_id="HGU4197466.1"
                     /translation="ERTVIKNPEYFSTYMQEELKALV"
BASE COUNT         2734 a         2767 c         2786 g         2648 t
ORIGIN      
        1 atacactaaa catagctttg gctaaattca taacttattg agttgaaacg agaagcttat
       61 aaattttttc cagatcgtcg atatttcgaa cgcggattaa cagccgacgc tgttctaatt
      121 gcatcaccaa cacgccatct tccgacaagt tcatcgcttt gattcggcta tattcaatcc
      181 agacattggc gaagaaaaaa cctttttgtt taaagatgat cttcggaacg cggatccaga
      241 atatataaaa acccatcaga gccagtgcgc ttaataacca tgtcgttatt aacgcaccat
      301 gattcgtgac gttgttatag ataagaatga caatcaatcc gacgaagata acgctatcga
      361 tgcgaccacg ccggagcaaa ggaattgcca gcagggtggg gccgttacgg cggggcatga
      421 tgaactgatc gtagatcgcg aaggccagga gtgcggcgat gaaaagaatc agtaccaggt
      481 ccgtgattgt cattaatcct ccagataaaa aaatggggcc aaaaggcccc ggtagtgtac
      541 aacagtctta cagtcccagc aggccgcaag cgtaaccagc gataccgatg acgaagaagc
      601 caacgatgat ccacagcggg ttaacttttt tgcgcagtag ccacatacaa gcaaaggtca
      661 gcagcagtgg taccaggcct ggcattaact ggtccagaat agtctggaca gtagtaacgt
      721 gttctttgcc cgtctggtca gtaatgcgag agacaaccag cgggatgttg acatgtgtcc
      781 acttgttaac caatgccccc atgacaaaca ggccgaggat agacgcccct tccgtcagtt
      841 tttgcaggaa gccaccaccc atatctttaa cgatatcgat acctttggag taaccatacg
      901 ctacgccgta gtaacgggtt gccagacgca ccaggttaaa caggatgaag aacagcagcg
      961 gacctaacag gctgccgctc atcgcgatac cggcacccag tgctgcaaat accggacgta
     1021 cggttcccca gaagatcggg tcgcctacac cagccagcgg ccccatcaaa ccgactttga
     1081 taccgttgat agcaccgtcg tcgatctctg cgccattagc acgctgttct tccagcgcca
     1141 gggttacgcc gagaatcggc gcagccacga acggctgggt gttaaagaac tccaggtgac
     1201 ggcgaatagc ttgtttacga gcttcgttgt tctcagggta gaggcgacga attgccggta
     1261 ccatagagaa gcagaaaccc agtgcctgca tacgttcgaa gttccatgaa ccctggaaga
     1321 ggttagaacg caggaagacg ccacgaatat cactttgagt gagttttttc tcggtggtag
     1381 tttgagttgt atcaaccatt tcgctcacct gttagtccag ttcgttatcg agatcgttgt
     1441 taccagctgc ctgagcaggc gcaccggcta cgcggttgta tttcgggcta agttggatgt
     1501 agagcactgc cataacagta ccaatcacac ccagagcaac caggttaaag ttggtgaatg
     1561 ctgcggttac gaagccgagg tagaagaacg gcatcaggta gccagcacgc atcatgttga
     1621 taaccatcgc ataaccaacc accacgatca tgccaccggc gatattcaga ccattggtca
     1681 ccacttccgg aatcgcattc agcatgttct gtacttcgct ggtaccaaca gacagcgcaa
     1741 cgatgacggc cggaatagcc acacgcattg cttgcaggaa cagagaagaa acgtggatcc
     1801 aggaaatcgc tgtcaggttg ccgttatcag cagccttatc cgcagcgtgc tggaaagcaa
     1861 cggtaatagt acgaacgatg atggtcagta cctggcccgc agcggccaga gggattgcca
     1921 gtgcgatacc tgcaccaatg ctctgatgac ctgcgataac cagaatggta gaaatgatag
     1981 aagccagagc ggcgtcaggc gcaactgcag caccgatgtt catccagccc agcgcgatca
     2041 tttccagcgt accaccgata ataataccgg ttttcatatc cccaagaacg atacccacca
     2101 gggtacacgc gattagcgga cggtgaaact gaaattcatc gaggattgat cccatacctg
     2161 cgatacaggc tacgataaat accagcacaa tttgaagagt ggtaatctcc attgtacttc
     2221 tcctgtttac gtaagactta agtgtgaaaa ctgagtgata atcaacacaa tacgttactt
     2281 atcgattttg ctgatcagat ccatcatttt cagtttcgga tcggtggaaa ccttacggac
     2341 ttccagctca ataccgcgcg cattcagttt cttgaacgcc tcgatatctt tttcatcaac
     2401 cgaaaccgcg ttattcacct gggttttacc ctgacggaat gccataccac cgacgttaac
     2461 agaggtgatt ttcacgccgc cttcaacgag acgctctaca tctgttgggt tggtaaataa
     2521 cagcattacg cgttcgccag catatttcgg gttgttgtag acgcgaatca ttttggcaac
     2581 atcaactacg tgtgctgtta cgcccggagg tgcaacctgg gtgagcagtg tcttacgaac
     2641 ggtatccgca gccacttcat cactaacaac aataatacgg gagacattgg tttctttggt
     2701 ccagcgggtg gcgacctgac cgtgaatcag acggtcgtcg atacgcgcaa ggccaataac
     2761 catgtagtcg tttggcccca ttggttttgc cggagttgga gccgcttttg gtgctgcggc
     2821 agcgggtgct ggcgcggctt tttcaaccgg tttggctttc agagctttca cgccttcacg
     2881 gcctgtttct actgccagtg ccaccagttc atcaaagctt gggtcatcat cacgggccat
     2941 taacgtttcc acgagcattg gaatgttaac gcctgcaatg acttcataat gctctttgtc
     3001 gacgacaatg cggctggcag cattgaacgg gctgcctccc catgtatcaa cgagaaacag
     3061 cacgccttta gtggtgtcga gttttgccaa ctgagcgttg tacttttcaa tcagcgtttc
     3121 ggcattttca cctggaacga aatcgatcca gccgacgttt tcctgctcgc ctaacagcat
     3181 ttctgccgtt ttaagcaact gctctgcagc ccaaccatgt gtgcctataa caatagcaat
     3241 ggtcacttgc tacctccttt attatcgtta acacctcaac gtgccagatg tatttttgaa
     3301 tcgtcgtcca caatcgaatc gattcagata agggctacaa ccaaaaaccc ctgcgtttcg
     3361 cgatttattt tatatatcga aaaaattatt ttatgtgatg aagatccgta atttaacttt
     3421 cgattagcag aaatttcgaa aggtaaaata tccttgtcac attcgtttgc aaaggaaggt
     3481 aaatctttgc caaatcagag gcgtctctga tatgtttaac tcccgtttaa caaccatgga
     3541 gtatagggca gtagcccgca gtatggatcg tcaccgacgt catttcagca tcaggccttt
     3601 taacgcctgc ctttctggct ctctatgccg cacctttcgt ttgcattttg tcgttacgcc
     3661 tgcattattt ctggcgtcga atagctattc cttaagcagg agcttgtcat ggaattctta
     3721 atggacccct caatttgggc ggggctactc acgcttgttg ttctcgaaat tgtgctgggt
     3781 atcgataacc tggtcttcat cgccattctt gctgacaaac tgccgccaaa acaacgcgat
     3841 aaagcgcgtt tgctggggtt atcactggcg ctgattatgc gtctggggct gctgtcgctg
     3901 atttcatgga tggtcacgct gaccaaaccg ctatttaccg tcatggattt ctccttctcc
     3961 ggacgcgacc tgattatgtt gttcgggggg atattcttgc tgttcaaagc aacaaccgaa
     4021 ctgcatgaac ggctggaaaa ccgcgatcat gattccggcc acggtaaagg ctacgccagt
     4081 ttctgggtgg tcgtcacaca gattgtcatc cttgacgccg tcttctcgtt ggatgcggta
     4141 attactgcag tagggatggt taaccatctg ccggtgatga tggcggcggt agtgattgcg
     4201 atggcggtta tgttgctggc atccaaaccg ctgacgcgat tcgttaacca gcaccccacg
     4261 gtggtggtgc tctgtctgag cttcctgtta atgattggtc tgagtctggt ggcagaaggt
     4321 ttcggtttcc acattccgaa aggttacctg tatgccgcga ttggcttctc gatcatcatc
     4381 gaagtgttta accagattgc gcgtcgcaac tttattcgtc accagtcgac tttgccgctg
     4441 cgagcgcgta ctgccgatgc catcctgcgt ttgatgggcg ggaaacgtca ggccaatgtt
     4501 cagcacgatg ccgataaccc gatgccgatg ccgatcccgg aaggtgcatt tgccgaagaa
     4561 gaacgttaca tgattaacgg cgtactgacg ctggcgtcgc gttctctgcg cgggatcatg
     4621 acgccgcgcg gtgaaataag ctgggttgac gctaatctcg gggtcgatga aatccgcgag
     4681 cagctgctct cctcaccgca cagcctgttc ccggtatgtc gcggtgaact ggatgaaatc
     4741 atcggaatcg tacgtgctaa agaactgctg gtggcgctgg aagagggcgt tgatgtggcg
     4801 gcgattgctt cggcgtctcc ggcgattatc gtcccggaaa ccctcgatcc gatcaacctg
     4861 ctgggcgtgc tgcgtcgcgc tcgcgggagc tttgttatcg tgaccaacga gtttggtgtg
     4921 gtacaaggct tggtcacgcc gctggatgtg ctggaagcca ttgcgggtga attcccggac
     4981 gctgacgaaa cgccggaaat cattaccgat ggtgacggct ggctggtaaa aggcggtaca
     5041 gatttgcatg ccttgcagca ggcgcttgat gttgagcacc ttgccgatga cgatgatatc
     5101 gcgacggtcg cgggcctggt gatctcggca aatggtcaca ttccccgtgt gggcgatgtg
     5161 attgatgtag ggccactgca tatcaccatc attgaagcca atgattatcg tgttgatctg
     5221 gttcgcattg ttaaagagca accggcgcac gatgaagatg agtaagcgtt aacgtaacgg
     5281 cataatgggc gtgatatgtc cattatgccg ggcgggcggc ggttggctgc ccgccagcca
     5341 tttgggaaaa tcacgtagcg gcatcggctg tgcgtacaaa tacccttgca aaatatgcac
     5401 gccatgacgg cgcaaatact tcgcctgttc ttgcgtttcc acgccctccg ccaccagttc
     5461 aatattcagt ctttgcccca gcgcgatgat gatatcggtc accgtcgagt taaccgcgtc
     5521 agaacctata gctgcggtaa atgacttatc aattttcagc acgtcaggac gtaatgtttc
     5581 aagccaggaa aatgaactgt tgccggtgcc gaagtcatca atcgccagtt tgacgttttt
     5641 acgatgtagc tcgcgggcaa tccgataatc aacatccagt aaggcatcgc gttcggtgat
     5701 ttcgaggatc agttgctgaa tcgggtgagc gctaaaccag tactgattga gatcttttat
     5761 cagcacacca cggcgaaaat ggctgggtgc gacgttaatg ccaacatgaa actgactact
     5821 catcgggaaa acatggcgct gacgaatggt ttctgccatc acatagcggg tcagtggcac
     5881 aattaaatga tgttcttccg cgataggaat aaacacatcc ggtgaaatcc agccctgacg
     5941 cggattgttc cagcgcagca aaatctctac accaatacat tgctggctgc gcgcattaag
     6001 caaaggctga cagaacaatt cgaactcatg ttgcgccagg cccagattga tttcgcggga
     6061 aaagctcatc cggtaagcgg tcgccagcca ggcgatgtag cccaccagta aacttagcag
     6121 caccgccagc ggtaattgtg ttggaagata gtgccatgcc agcgccgtag cccccggacc
     6181 attaacgtta atggtaaaag gaaagcgttg cgaagaaacc tgatagtttt cattgtcttc
     6241 aggttgcgga agggaatcta ccagcccatt accatagagc aaatgccgtt tgtccaccgt
     6301 caggctggcg ctactgattt gcggcagttg tggctcaagg agcattgccg tcagtaagtc
     6361 gatgttaatc atctccatga ccccagcatt gctactgccc gctgcaggcg tccattgaat
     6421 caaaaccgga ctgcctttaa tcagggcacg gtcgatcgtc aggcgtaaaa gtggttgcgg
     6481 tgcaggaagt tcagccagaa tgtccacgac gggcacattg cgataaccaa aaatgctgga
     6541 gcaataaagt gtgccgtctt gcaccaggcc aatggatcgc acagtttgga gttttgccgc
     6601 ctgtttacgt aatggcaaat gggcgacaga gcagggcaga ccaatcagcg gaagcaagac
     6661 ttcgctacct gcctgtaggg gaagcagtac tttatctaat tcctctacag cgtgattggc
     6721 gaattgtact acccgttgtt gatttaagtt acgctgtgaa ataaatcgca cgctcagggt
     6781 cgaagcgagc gtaacgagtg cgcaaatcgt acaaacaatc attcgattac ggcgataggt
     6841 tttaatgatc cgttgtgctt tttgcatgcg ggccaccctg taagcgtatg gcgacaaacg
     6901 cctgacgcaa cagtggaagt gtagtgggga atattacagc agacgagaaa gcgggtataa
     6961 attcgcccat ccgttgcaga tgggcgagta agaagtatta gtcacactgg actttgattg
     7021 ccagaccacc gcgtgaggtt tcgcggtatt tggcgttcat gtccttaccg gtttcgtaca
     7081 tcgtttcgat gaccttatcc agcgagacgc gcggtgcact ggtgcggcgc agagccatcc
     7141 gtgcggcgtt aatcgccttc acagaggcaa tggcattacg ctcaatgcac ggcacctgaa
     7201 cctgccctgc aaccgggtcg caggttaaac caaggttgtg ttccatgcca atttccgccg
     7261 ccacgcaaac ctgttccggg ctaccgccca gcagttctgc aagacccgca gcagccattg
     7321 aacaggcaac acccacttcg ccctggcaac caacttccgc accggaaata gaggcgttca
     7381 ttttatacaa tgcaccaatc gcgcccgctg ccataaagta acgggtatag atgtccgggc
     7441 tgaccgattc aataaagtgg tcatagtaag ccagcactgc cggaacgata ccgcaggcac
     7501 cgttggttgg cgcagttacc acacgaccac cggcggcgtt ttcttcgtta actgccagcg
     7561 caaacatgtt tacccagtca atgacattca tcggatcgtt agacagttta tcgctggaaa
     7621 ccagcatccg gcgcagggca gacgcacgac gtggcacgcg cagcgggcct ggcagtacac
     7681 cttcggtgtt catcccgcga tcgatacatg cctgcatggt ttgccagaca tgcgcgaaat
     7741 actcgtcgat ctctttcttg ctgtgcagcg ccagttcgtt ctgcatagcg agaccagaca
     7801 gcgaatagcc ggtttcatta cagtacgcga gcagttcggt ggcagatttg aacggatacg
     7861 gcacgctcac ttcgttggca gcatcctgac caaagtgttc ttcatcgacg ataaaaccgc
     7921 cgccgatgga ataataagtt ttgctgtaga cgacttcatc gccgttatag gcgtggattt
     7981 gcataccgtt ttcatgcagc ggcaggttgc cgttatgaaa acacatcccg ttgtcgcgcg
     8041 ggaaatccac ttcatgccgt ccctgtgcca gcagcagacg ttcgcgctct tctacgtcgc
     8101 gaataaaacc gggaatactg tcgatatcca cggtggcagg ttcgttacct gcaagaccca
     8161 taataatggc gatatcggtg tggtggcctt tacccgtcag cgacagtgaa ccataaacgt
     8221 ccacggcaac gcgagtaacg ctatccagta agcctttttc gaccagatca tcgacgaact
     8281 gtttacccgc cttcataggc cctacggtat gggaagatga gggaccaatc cccaccttaa
     8341 acatgtcgaa tagactaatc acgataatac tcctgacaag gtgactggac ttccagtaac
     8401 gaactaataa cagcgcatag tgtaagaggg aacgcggcga ctggcttaac tattcacatg
     8461 aattaaacta atcaaaaacc gcgatttaac taatattgcg atgattgtct caagtagtgt
     8521 tttgtagatg taaagataag tatagtcagg gtttcacacc aatttgcagc gccagctcac
     8581 gaattatgcc tgcggtcatt ccccatacaa aatactgttc gtaccaggac agccataccc
     8641 gatgtgaatc gccacggcgg tagatatcta aagggtgata acgacccaga tgtaatgcct
     8701 gggcgagcgg catttcaaac accgccgaga cttcatcttc actggcgcga tacggcagat
     8761 cgggcgggat aatgccgacc actggggtta cctgatagcc agttacgcta tcgacgggcg
     8821 gcagcacgcc gataacttca acggcggaag gcggtatagc gacctcttct tcagcttcgc
     8881 gcagcgcggc ggcgataact gatgcgtccg tgtcatcgac tgcacctcca gggaatgcca
     8941 cttgtccagc gtgtttacgc agatgaattg aacgctgagt cagcaacaac cccggttgcg
     9001 gtcgacggac gatggggatt aacacggcag cctgacgatg atttagggtt tcccggttaa
     9061 tttgcgggcg caaaagttga aagcgagata aaaaataatc aagcgtcagg ctacggtatt
     9121 ccacgtctta cttctccagt tggcgtaata tcttattaac tttatcaaaa gtttcctgat
     9181 attccgcttc ttcctggcta tcggcgacaa ttccaccgcc cgcagagcag tatatttgtc
     9241 cgttaatggc agtcagcgtg cggatagtaa tgctggtatc catgttgccg caaaagctca
     9301 aatagccaat gctgccgcac caggcattac gtcgctgcgg ttccagttcg tcgataattt
     9361 ccatagcccg tactttcgga gccccggtta ttgagccacc aggaaaagct gcgcgcagca
     9421 gatcgctggc gtgtaactgt tctggtagtc gcgccgttat agtgctgacc agatgatgca
     9481 cggcagggaa gggttccacc acgaacagct ctggtacttt taccgaaccg gctacggcaa
     9541 cacgaccgat atcattacgc attaaatcga caatcatcag attttcggca cgatctttcg
     9601 ctgagttcgc cagtttttct gcttgtttgc tatcttcctg aggatcgggc aggcgtggta
     9661 gcgtgccttt aatcgggcgg gtctggattt cactattatc acaaagaata aaccgctctg
     9721 gcgaaaggct taaaattgca ccctgttcaa gacgtaaaaa agcgctaaat ggcgcgcggt
     9781 tggcctgatt aagctgaagg aatgcctgcc attcatcgcc agaataggtc gcatgaaaac
     9841 gctgggcgag attcacctga tagcaatcac cgctgtgcag atattcctgt acctggcgaa
     9901 atttttcgcc gtactgctcg cgggtcatat tggattgcca gtcggaagtg agcgtgaaat
     9961 cttcctgcgg cgagaattgc tggctttcca gccaggcccg acgggcattg acatcattat
    10021 gactcagcaa agaaactgta tgacgctggt ggtcgacaat gagcgcccaa tcgtagatac
    10081 ccactgccat atccggcaga acgatatctt gttccgcaat ttctggcagt gactcaaaac
    10141 ggcggcccag atcgtagcca aacaacccca gtgcgccgcc ctgaaatggc aaatcttcgt
    10201 tatgcgttgg gcgaatgtct gcgcgatcca gcacctgctg gagcacctgt agcgggtcat
    10261 cagtggtcgt tgtgtgtttt tcgctttcac taacaacggt ttctttaccg aaagtggtta
    10321 aagtgcaaat cggatcggcg accacaatat caaagcggct atacggatga tcggcatagc
    10381 cggagtgtaa aagcatcgcc cacggcaggt ggcttaagcg ggagaaataa aattcagcgg
    10441 cgtcctgacg ccagggtaaa gtaatcacag cgggagataa cgtcttcatc agtcctgact
    10501 ctactggcta tgtgccgggt agcatgaaat aattagcgcc aacaatttag caggagttaa
    10561 caatgtttgc aggtttacct tcactcaccc atgaacagca gcaaaaagct gtcgagcgga
    10621 tccaggaact gatggcacaa gggatgagca gcggccaggc aatcgcgctg gtggcagaag
    10681 agctgcgtgc caaccactcc ggcgagcgaa tcgtggcgcg ttttgaggat gaagacgagt
    10741 aacacactaa tccgtatcga tttcgttata gaatccgatg attatttgat tgtgagaaaa
    10801 aggacgcttt ttatatgtga ttaaaagcgt cctgacccat ttatgctgat ataaattaag
    10861 ctcagaccag tgcttttagt tcttcctgca tatatgttga aaagtattca ggatttttaa
    10921 ttacagtgcg ttcac
//
LOCUS       DAZFVO020000159        10885 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFVO020000159 DAZFVO020000000
VERSION     DAZFVO020000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.160"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..2131)
                     /locus_tag="ACHKNB_RS20270"
                     /old_locus_tag="pgaptmp_004054"
     CDS             complement(<1..2131)
                     /locus_tag="ACHKNB_RS20270"
                     /old_locus_tag="pgaptmp_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HGU4197467.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHA"
     gene            complement(2146..2466)
                     /locus_tag="ACHKNB_RS20275"
                     /old_locus_tag="pgaptmp_004055"
     CDS             complement(2146..2466)
                     /locus_tag="ACHKNB_RS20275"
                     /old_locus_tag="pgaptmp_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HGU4197468.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            complement(2602..3057)
                     /locus_tag="ACHKNB_RS20280"
                     /old_locus_tag="pgaptmp_004056"
     CDS             complement(2602..3057)
                     /locus_tag="ACHKNB_RS20280"
                     /old_locus_tag="pgaptmp_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197469.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPVSRAAPVAREWREIPRTEPDPETTPDNSYRNYL"
     gene            complement(3050..3337)
                     /locus_tag="ACHKNB_RS20285"
                     /old_locus_tag="pgaptmp_004057"
     CDS             complement(3050..3337)
                     /locus_tag="ACHKNB_RS20285"
                     /old_locus_tag="pgaptmp_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HGU4197470.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            complement(3330..3929)
                     /locus_tag="ACHKNB_RS20290"
                     /old_locus_tag="pgaptmp_004058"
     CDS             complement(3330..3929)
                     /locus_tag="ACHKNB_RS20290"
                     /old_locus_tag="pgaptmp_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HGU4197471.1"
                     /translation="MMMPALQKVPFSGLPFSGICGYSFSAVAKSAAGRGNPSNLTATY
                     DAPCVFFCVAINATERQIMVWCVVSRADGTPCILPASAHYAAESMVAQAGQPPGWPVS
                     VRAGISTPVWAIAIERGNSGDSVTCYLTEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            complement(3926..4192)
                     /locus_tag="ACHKNB_RS20295"
                     /old_locus_tag="pgaptmp_004059"
     CDS             complement(3926..4192)
                     /locus_tag="ACHKNB_RS20295"
                     /old_locus_tag="pgaptmp_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4197472.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            4744..5412
                     /locus_tag="ACHKNB_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /pseudo
     CDS             4744..5412
                     /locus_tag="ACHKNB_RS20300"
                     /old_locus_tag="pgaptmp_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
     gene            complement(5464..6750)
                     /locus_tag="ACHKNB_RS20305"
                     /old_locus_tag="pgaptmp_004061"
     CDS             complement(5464..6750)
                     /locus_tag="ACHKNB_RS20305"
                     /old_locus_tag="pgaptmp_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4197473.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHINAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            complement(6784..7038)
                     /gene="xisR"
                     /locus_tag="ACHKNB_RS20310"
                     /old_locus_tag="pgaptmp_004062"
     CDS             complement(6784..7038)
                     /gene="xisR"
                     /locus_tag="ACHKNB_RS20310"
                     /old_locus_tag="pgaptmp_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4197474.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(7057..7191)
                     /locus_tag="ACHKNB_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /pseudo
     CDS             complement(7057..7191)
                     /locus_tag="ACHKNB_RS20315"
                     /old_locus_tag="pgaptmp_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7195..7437)
                     /locus_tag="ACHKNB_RS20320"
                     /old_locus_tag="pgaptmp_004064"
     CDS             complement(7195..7437)
                     /locus_tag="ACHKNB_RS20320"
                     /old_locus_tag="pgaptmp_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4197475.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            complement(7531..7602)
                     /locus_tag="ACHKNB_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /pseudo
     CDS             complement(7531..7602)
                     /locus_tag="ACHKNB_RS20325"
                     /old_locus_tag="pgaptmp_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(8614..8792)
                     /locus_tag="ACHKNB_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /pseudo
     CDS             complement(8614..8792)
                     /locus_tag="ACHKNB_RS20330"
                     /old_locus_tag="pgaptmp_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8890..9342)
                     /locus_tag="ACHKNB_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /pseudo
     CDS             complement(8890..9342)
                     /locus_tag="ACHKNB_RS20335"
                     /old_locus_tag="pgaptmp_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            complement(9339..9579)
                     /locus_tag="ACHKNB_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /pseudo
     CDS             complement(9339..9579)
                     /locus_tag="ACHKNB_RS20340"
                     /old_locus_tag="pgaptmp_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9572..9775)
                     /locus_tag="ACHKNB_RS20345"
                     /old_locus_tag="pgaptmp_004069"
     CDS             complement(9572..9775)
                     /locus_tag="ACHKNB_RS20345"
                     /old_locus_tag="pgaptmp_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197476.1"
                     /translation="MSVIKTHTGIVITRDGPQVKKLHQTKRMWVVGKNEFYHKETGRR
                     HFAENTRRRLLIDTIKPIEVKHV"
     gene            complement(9772..10134)
                     /locus_tag="ACHKNB_RS20350"
                     /old_locus_tag="pgaptmp_004070"
     CDS             complement(9772..10134)
                     /locus_tag="ACHKNB_RS20350"
                     /old_locus_tag="pgaptmp_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197477.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPKNKK"
     gene            complement(10125..10661)
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS20355"
                     /old_locus_tag="pgaptmp_004071"
     CDS             complement(10125..10661)
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS20355"
                     /old_locus_tag="pgaptmp_004071"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4197478.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(10790..>10885)
                     /locus_tag="ACHKNB_RS20360"
                     /old_locus_tag="pgaptmp_004072"
     CDS             complement(10790..>10885)
                     /locus_tag="ACHKNB_RS20360"
                     /old_locus_tag="pgaptmp_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197479.1"
                     /translation="VQEEMANEFRDLLIEKFKDSKVETFIGTFTA"
BASE COUNT         2484 a         2879 c         2762 g         2760 t
ORIGIN      
        1 aggcgtgata gaggtattta cggtaattac gcgggatgat actggcgttc cccatataca
       61 tgccgctggt ctgcggcagg gtttccagat agccgataaa atcaaacgtc gggtcggcat
      121 cccgtttgat gttcagtgcc tcgtctgagt tctgctggga ctgaagcagt gaccgggcga
      181 gcatcgggtc gctgaacttc tgcatcaggt gacgcacgat gaccgccagc tcgcgggtga
      241 ttttgtcctt aagttgcggg tcgcgctcct gcggggctat ctgttccggg aagtgaataa
      301 tcacccgccg gcgtgacacg ccgccgctgc ggtcggtgaa gcgcatcggg ttattgttca
      361 cggccagaat caccgccggg atatgcgtgg agtacgcatc ccggtatttc gggtccacgg
      421 acaccgcatc gccgccggtg atggccttga gtccggcacc gtcgccgctc catttttcct
      481 ggtccggcag gcgtatcagt gagaagccag ttaacgcggc acgttcacgc ggggattcca
      541 gcgtctcaat ggtggccgac gtggcgttat cctccccggc cagcagggtg gctatttcgg
      601 ccatgatact tttgccgctg ccgccgggac cggtcacctc cagaaagagc tgccagtcat
      661 agcggtttgc cagcaccata aacagtgcgg ccagaatcac gtcgcgtttt tccgcacggc
      721 caccggcggc acggtcaagc cagcgccaga acgcgggggc gtgggtttcc agcgtttccc
      781 cttccaccgg cggggtgaaa tccacatcgc acagggtgcg catccagtgt gacggactgt
      841 gcgggtggaa cgtgccgttc tgcgtgtcga gcacgccgtt acgaaagcca atcaggcggc
      901 gggagggggc ttcctgctgc ggaataatca gcttcagggt gtccaccacg gaggccacct
      961 tcccggagga gaacggcgcg cgcagacgct gaaacagccc ggccacatcc cgggcaaagt
     1021 cctgtggcgg cagcaccttc cagacaccat tttcataacg ggacagaagc tggccgttgg
     1081 catcaaccgc gagcgcctcg ccgtaatgct catagatacg catggccttt tcgctggtac
     1141 tcatggcgga aaactccgct tcgctcatgg tatcgaacgg gctttcagcc ggtggccgga
     1201 tggcatcgta aatggcctta cgggtggctt ccccgccgta ctgcgtgaag gcatcattcc
     1261 agtcaccgaa gaccggcggc agggcaacaa caccttcaca cgcatctgcg gctgcggcgg
     1321 cttttttctg gccgtcacca ctgaggtcac ggtcagcggc aaggacaatc tgacaggcgg
     1381 ggtgcttctg ccgggcaagg ctggccagag aaaggaggtt cacggaagaa agcgccacca
     1441 tcaccgtttc accggtcagg tgatgtacgg taagtgcggt cgcgtatccc tccgctatcc
     1501 acagacgttt tccggcctga ttctgtcctt caagggtgtg acaggtgccc ctgacctgtc
     1561 cgcctttcag ggtgcgctta cggccgtcag cactgattaa ctgaaggtta accagttcgc
     1621 cgctgtcgtc atacagtggc accacaaggt caccggcgcg ccagctcacg ccaccggctc
     1681 tgtgtgtgcc ggtcagcatc cggcattccc ggccgggaaa gcccttgcgg gtcaggtagg
     1741 cgttaccggt tccgggacgg gtttttgcca tcagggtttg tgccagtgcg gcggcgttct
     1801 tccgggcagc gtctgtttca gcgccggcgg cggccgtcac tgccgggtca gccgggggca
     1861 ggctgccggt cacggcagcc acctttgcgg ccgcgtcgga cggggaaaca ccaaacacct
     1921 tttcaaccag tttcaggccg tcaccggcac cacactgatt gcagtaccag gtgccgcgcc
     1981 cctccctgtc atcaaaacgg aaacggtcac tcccgccaca gaccggacag ggctgatgac
     2041 ggtttttcag cacctgaatc cccagcgccg ggagaatacg cggccagtgg ccgagtgcat
     2101 ggctgacggt ggcggttacg ttcattttca tggtgttgtt ctccttcagt gcagtaccgg
     2161 cgcttttatg tgacgggcac agagttcatc catcacaacc agcccgagaa aggacagcga
     2221 cggcgcggcc ttcagggggc cggattccat taaatcttcc agcagggcac aggctatctg
     2281 acgccctttt tcctcaccgt gctggcgcag ataaaaacct tccagctcag cggcgatggc
     2341 cgcctccagt gactcaaggg tgagatgcgg gtagcggtgc tgacgttcgc acacggtcag
     2401 ccaggcacag gcgacagcgc gacggtaaag ggcagcgcgt aagacgggcg gtaagggtgt
     2461 tttcatttgc tttcctccct gtgacagatg actgcattcc gtgccggttg cattaactga
     2521 taaggcatat ctgcgtctcc tgaagacgtg cgtatccctg cgcgaatacg cacatttaat
     2581 ttttcgggtg tcgtttttta attacagata attgcggtaa ctgttatccg gggtggtttc
     2641 cgggtcaggc tccgtgcggg gaatttcccg ccattcccgc gccaccggtg ctgcccggct
     2701 gaccggaaca gtgtcctgcg ggtaaatatc cagatatttc tcccgccatt tctgtaattc
     2761 cgggtctccg gccatttctt tcagtaccgc atgccggttt acggggctgc gtttaaacag
     2821 gtcaggacgg tcacaggtaa attcccgcag aaaacgcccc agcgggatgt ctgtggtgcg
     2881 cccgtcggcg aggatacgca caaggatact gaatttacgg cggtacggat tccagacaat
     2941 gtccgggcag cggtacggca tttcccacgg aataccgtct tccagaatgc cgaccacggc
     3001 cacatcggga aaaccggcag aacggtaaat ctcaccgggc tggggaaaat caaacatgcg
     3061 tcctgtctcc ccggtctttc tgctgggcga gaaaatcgcg gcacaggcct ttggctttca
     3121 gctcattcag cacaaaatca atatcttcat tcaggtagct gaaaatatgc ggaatgtaga
     3181 gctgatgcag gccggagagt tcacggtgaa tcaaatcacc cccaacaaac tgggatacgg
     3241 cgctggcgcg gttgagctta tggtaagcct caatgctgag gtgttcacgg gcgtcatgac
     3301 gcgctgagac ggtctgaggg gcttttttat tacgcacggg acacctccac caccggcaga
     3361 cgggcagcaa gggagagcac atagtcacgg acaagggaac ggcgggcact gcgttcatca
     3421 ccggcgacgg tgcgaagcat gcagatacgg ggatgacggt ctgcgcgacg gacagccgca
     3481 aacacaaaga caaattcagg gtgtgaaggg gtaagggttg tagccatgat ggcagcctcc
     3541 gttagatagc aggttacgct atcgccggag tttccacgct cgatggcgat agcccagacg
     3601 ggggtggaaa taccggctct aacggatacc ggccagcccg gaggctgccc cgcctgagct
     3661 accattgact ctgcggcata atgagcggac gcgggcagga tgcacggagt gccatctgct
     3721 cgactgacca cacaccacac cataatctgg cgctctgtgg cgttgattgc gacacaaaaa
     3781 aagacgcatg gcgcgtcata tgtcgccgtt agattgctcg ggtttccacg cccggctgcc
     3841 gattttgcga cagcggaaaa actatatccg caaatgccgg aaaaaggcaa gccagaaaaa
     3901 gggacttttt gcagagcggg catcatcatg cgtcgtaccc ccgtttgcgt ccggcaatgc
     3961 gtccggccat ccatgcggtg acttcagagt gcagccaggc cacattttta ccgccaagac
     4021 tcacctgcgg cggaaattcc cccttacgga tgagttcata gatggtcgag cgtgacaggc
     4081 cgcacaggtg catcacttcc ggcagacgta aaaaacgctc ctgcgtgatg tccggcagcg
     4141 gcatcagtgg cgtcacaggg gcgggagacg gggaagaaaa aacagcttgc atcgggctac
     4201 ctcgttaatg tccatacagc accggataag tccgtccggc ttcgggtagc gctttatttt
     4261 gtgaatattt ttggcagacg caacaggggg gatttgttcc ggcagcctta caatggctgt
     4321 gcattttttg tacatcagcg ccagatagct ttaaaacgct ctggaaggag ctggaaaaaa
     4381 ttatagtgaa atacaaattg ttttttctta tttatttcag tgaattaata aaaataaaaa
     4441 gtaataaaca gcacaaaaag cccatcaacg ggtgaacagt ggtgaacaga cggtgaacag
     4501 tcattactgc gattgttcac cctttaactt attgtattac ttatcttttt tcttatggtg
     4561 aacagaggtg aacagtaaaa tataaaaaaa caaacagtaa gccggttttt cctgcgacct
     4621 tttcctggct tgccggtctg aggatgagtc tcctgtgtca gggctggcac atctgcaatg
     4681 cgtcgtgttg ttgtccggtg tacgtcacaa ttttctcaac ctgaagtgac gaggagccgg
     4741 aaaatgtctg acaacaccat ccctgaatat ctgcaacccg cgctggcaca actggaaaag
     4801 gccagagccg cccaccttga gaacgcccgc ctgatggatg agaccgtcac ggccattgaa
     4861 cgggcagagc aggaaaaaaa tgcgctggcg caggccgacg gaaacgacgc tgacgactgg
     4921 cgcacggcct ttcgtgcagc cggtggtgtc ctgagcgacg agctgaaaca gcgccacatt
     4981 gagcgcgtgg cacgccggga gctggtacag gaatatgaca atctggccgt ggtgctgaat
     5041 ttcgaacgtg aacgcctgaa aggggcgtgt gacagcacgg ccaccgccta ccggaaggca
     5101 catcatcacc tgctgagtct gtatgcagag catgagctgg aacacgccct gaatgaaacc
     5161 tgtgaggcgc ttgtccgggc aatgcatctg agcattctgg tacaggaaaa tccgctcgcc
     5221 aacaccaccg ggcatcaggg ctacgtcgca cccgataaag ctgtcatgca gcaggtgaaa
     5281 tcatcgctgg aacagaaaat aaaacagatg caaatcagcc tcaccggcga gccggttctc
     5341 cggctgaccg gactatcagc ggcaacactc ccacacatgg attatgaggt ggcaggcaca
     5401 ccggcacagc gcgtaacgtg acagggatta agggtaaact aactgatttt acaagttttt
     5461 attttacccc actttcttat ggggcataca tgggacactt tcagatagtc ttttgttaag
     5521 gagttctatc tgttcgtgat tgttgtcttt catccatgct ccgtaaacat tgaataccat
     5581 ttgtgcgttt atgtggccca tctggcttgc gataaaacta ggattagctc cagcggcaag
     5641 tgaccagcat gcataagtat gcctggattg gtacgatttt ctgtgtctca gacctgcgcg
     5701 ttttaagata cttgtccatg actccctgat ggagtcaacc ttgtagtgag gtccggacaa
     5761 ctgctgctgt tttgttacct gaggactaaa aacaaaagtg catttatgca cagcagttct
     5821 cccatattcc ctctgcttca cctctacaga atgttgcttt ccaagcatgg tcatttccgc
     5881 ctggctttta agagcatcaa tagctggttg aaccagatga attgtccttc cggtgcctgc
     5941 atcggttttt ggtggagtga attcgccaag ttttgtataa tttctacgga tggttatagt
     6001 ccttgcttta aggtctatat cttcccatgc cagcgatacc aactccccgt gacgaatacc
     6061 cgtgtataca gcgagaatcc acaggttttt tgtttgttga tgacggcaag cctcaataaa
     6121 acgaataaat tcgtcacggg tgagaggatc tggttttacc ttggactttt ttaagggagc
     6181 cagaccgtta aatgggtttc ctgaggtata accattatct gttgcaaatt gaaacattcc
     6241 agctatggtt gtcatatagt agtttaccgt gaccactgag cgccctttta tggaagaagt
     6301 ctttccatta gaaagctttt ggtaaccggt caacaaatct ctccttacga aaagtaaatc
     6361 ctcttttgtt atggatgaaa ccagtttttt ttcacctaac attggtaaca tgtttttaat
     6421 tactgactgg taacggttaa gtgcattcgc acaaatctca attttcttaa ggtccaacca
     6481 tttttccgaa agtgccttaa cggttatctc tctttttccc agaccaaagt gtttcaggtt
     6541 aggggaatta gggaactgcg cggcgtagtc gaaactcccc attctgattg caaaacaaac
     6601 ggaagtgcga agttcaccag cgatcttccg gtttttggct gtgtcaggaa caccgaggtt
     6661 ttctctgaca cgtttgccat tatagtgaaa ccatatacgg agtgatcctc catggttttc
     6721 aacgcctgtc gggtatgatg cgttactcat taaacctccc agacgtccag gagcattaac
     6781 aggttaaccg gaacttgcat ttttggcacc tggttgtttc tggttttcga tccatcgcat
     6841 aatttcttcg atgttgtaca ggcattcact gtagtgcccc ggatcacctt ctacagcgta
     6901 atggcggtat tcttttccct gcatccatga ctttcttctt gcccgctcaa tggtgccagg
     6961 ctttagccct gttgatgcaa tgaggactct ctccgtacac catttgctgg gggttatctg
     7021 atagatgatt gtctgcatgc caacctcata aaattttcat ccacggcagt ggcaccacac
     7081 ttcaaccatt cgcttcacaa cttcacgaca gtagaagccg tcaacatctc gcgtcaggtc
     7141 atagcgattg ccgtaacgct ggtggactca tcgttcaaat gctttattca ttctttactt
     7201 cctttttatg gctcgtaatt ttttcaggtg cttttcctgc tcagtgtccg cgagaatttt
     7261 gcggtactcc tggtggtcaa tatgttcgaa caggcagttt aactcaccaa tgcgtacccg
     7321 cccggatcgt ccgtccatcc gtcgaaagaa cactgagtgc tcagtgatgc gagtaatcac
     7381 cacggggtat ccagctctgt ccgtgtatat ctgaccgcgt tgaatcaaag cgaacatgtg
     7441 gttatcccca tcgacaaatc gagaacacaa caaacgctgc tgcgaatacc acccccagag
     7501 ttacgattgc atcaggccag ctcattgatt cacctcctgc ctgtcgtccg gcattcgctc
     7561 actacagctt atccaaccat ccggagttac cggagagttg cccgatagtg cattctgctc
     7621 cagtgatgct tttacaaacc acgctgcctg aactataacg ccatgaatcc agcgcaaatc
     7681 agcatcgcga tctttctttt tcatcttttc gccacttaag gccttgctta tgtggctgcg
     7741 taccaggtct tcatgtaatt ccttcgcctc ctcaatggtg aaaccaccag gcagaagagc
     7801 cggagttacc ggagagctgg ttgacgcttc cgggatttcc cgaaaattat tggttgacga
     7861 attcttgctt tcccgaaagt ttccggactg aagcatggcg gtgcggcagg cgttccatat
     7921 ttcggcagca atatcgcgct cgctatcggt taatttgtac gtggaaacat agccagagag
     7981 catttctacg ttttccggag ttgcttcttc cggcactacc ggcgctggcg gggcggcaaa
     8041 tagatatccg ccaaagtcag gaagctctcc aatggcctgt acgaactttt gtttgcctac
     8101 gtcaactcct aatgggtaat gagctataat ctttgccacc ggctctgctt ccagcgatac
     8161 cagtgcaatt cgtgccagtt cttccgcttc ttctgctggc agtacaacgt tgctacccgg
     8221 tccgtatgtt tcgcgccact gcttgattgt cagcagtcgc cctttggtaa tagtgatcat
     8281 gccgcgtttc cttctttctt attaacaatc acaccgtcat atatttcatt aaggtgccct
     8341 ctcaactcca tgcgccttaa tgcagataac atgtaatcgc attcaacctg cttattccca
     8401 gtaaatggct tatcgtcagg attaccccaa cagcaattac ccctgggcca tccatgtact
     8461 ttccgtactc ttccgttaac aacgtgaagt aatccccagc cgggaggtaa atcctcaact
     8521 gaaataattt ccggctcact aataaagaat cgccagtcgc ccatgccaag tgagggattt
     8581 ttacggaaac gcttttttct atctgccaac aagtcagcac gagaacactt cgcctctatc
     8641 aggcatgatg ctgaatttct gaatcccata gcatctggct gttctccggt actggttaca
     8701 gcaacaaagc ggtcatgaaa gcaaaccttg aacccgttgc gcttaaggaa cttgtacgca
     8761 atctgacaga gttcgtggtg tgttaacgcc atatcactct cctttgatgc gaatgcctgt
     8821 tgcaatgctg tttatgatgc tgtcagtgca tggggtagaa agctgggcat ctccagcaat
     8881 tttcatgacc tcaacatctg catatcgaat accgaggtgt atcagaccgg ctatgcctga
     8941 cttaagccga gcattttcca taaatagaac ttttgcccgc tgtttttctg cttcaagctc
     9001 aacgcgcagc ttccctaccg ttagcgcaat atcctcgttc tcctggtcgc ggcgtttgat
     9061 gtattgctgg tttctttcct gttcatccag cagtgccagc acggtagccg ggttagcctc
     9121 tgctatgaat tcagcgtttg cataagcctg agcatctgtt tcaaccaggc agttaacgtg
     9181 acattctgca attacgccac cgggttctcc tttccatttt tgacaaacaa aaactcctgt
     9241 taaattgccg tgttggttaa cagatgtatg ccctacgatg tagcttcctt tagttgcttt
     9301 ctctgccttt tcacgcagta cctgatagtt aatgttgctc actggttgcc tcctttacgg
     9361 atctgcgctg cgatgcacga aaaaaaaaga ctttcgcgta tgactgttaa gagctggcgc
     9421 gaacgccgcg ttaagaacgg cggcatcaca gccgtcatcg atatagagcg caattttttt
     9481 ctccaggcgt gctttggctt cctgcaactg catatcccgg cacgcacgcg ggatatactc
     9541 agcaatttga gcgatagatt tttcgttctg tttaaacatg cttcacctcg ataggcttga
     9601 tggtatcgat cagcagtcgg cggcgagtat tttctgcaaa gtggcggcgt ccggtttctt
     9661 tgtggtaaaa ctcgtttttt ccgacgaccc acatccgctt tgtctggtgc agttttttta
     9721 cctgcggacc gtctcgggtg ataacaattc ctgtatgagt ttttatcacg ctcatttttt
     9781 attcttcggt gctttcggca ttactgccca gtgagtgata ttgacgtttt caaggtcccc
     9841 gacctgaaat gtccactgcc attctccggt ttctttttgt ccccaggtgt accagagaga
     9901 acgccagcca attagccagc cttctccgtt agcatcaaat aacagaacac tttcatttgc
     9961 tggcggcagt tcagctgaca ctggtattat tttgttttcc agtgccgcac atttagcttc
    10021 aagcgcatcg aatttacgta ccaggtactc agcatttgtt tcattcactt tcagatctcg
    10081 cggtacacat ttcccgcgaa gaaacccttc catttcgaaa acattcatgc gcatttgcgt
    10141 aactccgata actcgttaaa acgttccata aacatcccgt aggcatggcc tggtgacagt
    10201 ggaataactt tgaacatctc tgtcgccggg ataccttcca gtacaggcca gaaagagcca
    10261 tcatcaagcc cgagatcgcg acgttcggtt gccagcataa tgagatcggc atatttcaca
    10321 ggcgtgctca taacaggagg taacccgtat ttctcacgga ttacggcgtc tattttttct
    10381 tccatccgtt tatagtcagg aagaaggcgt ttcagtggag ctgggatgtc ctggcaatac
    10441 gcttctgttg catcatgcat taacgcttca aaagcaaatt cctgcggtac cagctggctg
    10501 caaagcaccg catgttgggc gacgctgtag aagtgtgaaa gatgtcctgc aaagcgacag
    10561 atatttgaaa gggaaaccgc gatatcgtta atcacgatgt cgtctttatt tatcctgtca
    10621 taataaaaat gcttcccgga aaaagtttta ataaatgaca ttttgttctc cactttatat
    10681 gcgctgcacc gcgctgaatt ttggttaaag aaaacccccg ccatcaggcg attattgagt
    10741 caattatgtt tccataaatg cccccgcagg ggcatttgca gtaatgaaat caggcggtga
    10801 aagtaccaat aaaggtttct actttgctgt ctttaaattt ctcaataagc agatcacgaa
    10861 attcgttagc catttcttcc tgcac
//
LOCUS       DAZFVO020000160        10859 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFVO020000160 DAZFVO020000000
VERSION     DAZFVO020000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.161"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..147
                     /locus_tag="ACHKNB_RS20365"
                     /old_locus_tag="pgaptmp_004073"
     CDS             <1..147
                     /locus_tag="ACHKNB_RS20365"
                     /old_locus_tag="pgaptmp_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HGU4197480.1"
                     /translation="VLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSML
                     KRIP"
     gene            327..971
                     /locus_tag="ACHKNB_RS20370"
                     /old_locus_tag="pgaptmp_004074"
     CDS             327..971
                     /locus_tag="ACHKNB_RS20370"
                     /old_locus_tag="pgaptmp_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HGU4197481.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            1058..1366
                     /locus_tag="ACHKNB_RS20375"
                     /old_locus_tag="pgaptmp_004075"
     CDS             1058..1366
                     /locus_tag="ACHKNB_RS20375"
                     /old_locus_tag="pgaptmp_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HGU4197482.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            1778..2758
                     /locus_tag="ACHKNB_RS20380"
                     /old_locus_tag="pgaptmp_004076"
     CDS             1778..2758
                     /locus_tag="ACHKNB_RS20380"
                     /old_locus_tag="pgaptmp_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HGU4197483.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            2751..3167
                     /locus_tag="ACHKNB_RS20385"
                     /old_locus_tag="pgaptmp_004077"
     CDS             2751..3167
                     /locus_tag="ACHKNB_RS20385"
                     /old_locus_tag="pgaptmp_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HGU4197484.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(3169..4443)
                     /gene="umuC"
                     /locus_tag="ACHKNB_RS20390"
                     /old_locus_tag="pgaptmp_004078"
     CDS             complement(3169..4443)
                     /gene="umuC"
                     /locus_tag="ACHKNB_RS20390"
                     /old_locus_tag="pgaptmp_004078"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HGU4197485.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMHLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLTQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(4443..4880)
                     /gene="umuD"
                     /locus_tag="ACHKNB_RS20395"
                     /old_locus_tag="pgaptmp_004079"
     CDS             complement(4443..4880)
                     /gene="umuD"
                     /locus_tag="ACHKNB_RS20395"
                     /old_locus_tag="pgaptmp_004079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HGU4197486.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(4877..5125)
                     /locus_tag="ACHKNB_RS20400"
                     /old_locus_tag="pgaptmp_004080"
     CDS             complement(4877..5125)
                     /locus_tag="ACHKNB_RS20400"
                     /old_locus_tag="pgaptmp_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4197487.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            5409..5720
                     /locus_tag="ACHKNB_RS20405"
                     /old_locus_tag="pgaptmp_004081"
     CDS             5409..5720
                     /locus_tag="ACHKNB_RS20405"
                     /old_locus_tag="pgaptmp_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197488.1"
                     /translation="MNYDGDGRATARFFSGKGCRRAPLFTAPADVARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            5830..6732
                     /locus_tag="ACHKNB_RS20410"
                     /old_locus_tag="pgaptmp_004082"
     CDS             5830..6732
                     /locus_tag="ACHKNB_RS20410"
                     /old_locus_tag="pgaptmp_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4197489.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEAVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(6683..6913)
                     /locus_tag="ACHKNB_RS20415"
                     /old_locus_tag="pgaptmp_004083"
     CDS             complement(6683..6913)
                     /locus_tag="ACHKNB_RS20415"
                     /old_locus_tag="pgaptmp_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HGU4197490.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLPEKKRAVTRPSPSYFIRHNAPPCRLSTQVR"
     gene            6829..7041
                     /locus_tag="ACHKNB_RS20420"
                     /old_locus_tag="pgaptmp_004084"
     CDS             6829..7041
                     /locus_tag="ACHKNB_RS20420"
                     /old_locus_tag="pgaptmp_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197491.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            7118..7801
                     /locus_tag="ACHKNB_RS20425"
                     /old_locus_tag="pgaptmp_004085"
     CDS             7118..7801
                     /locus_tag="ACHKNB_RS20425"
                     /old_locus_tag="pgaptmp_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HGU4197492.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            7802..8023
                     /locus_tag="ACHKNB_RS20430"
                     /old_locus_tag="pgaptmp_004086"
     CDS             7802..8023
                     /locus_tag="ACHKNB_RS20430"
                     /old_locus_tag="pgaptmp_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197493.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            8004..8471
                     /locus_tag="ACHKNB_RS20435"
                     /old_locus_tag="pgaptmp_004087"
     CDS             8004..8471
                     /locus_tag="ACHKNB_RS20435"
                     /old_locus_tag="pgaptmp_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4197494.1"
                     /translation="MRASKIEKETRMYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDIALTDHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVRENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQVRILLKNNTHEND"
     gene            8516..9286
                     /locus_tag="ACHKNB_RS20440"
                     /old_locus_tag="pgaptmp_004088"
     CDS             8516..9286
                     /locus_tag="ACHKNB_RS20440"
                     /old_locus_tag="pgaptmp_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197495.1"
                     /translation="MNETLNALIYRHASNLLLAQGWPEETDVDQRNPKYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            9702..10127
                     /locus_tag="ACHKNB_RS20445"
                     /old_locus_tag="pgaptmp_004089"
     CDS             9702..10127
                     /locus_tag="ACHKNB_RS20445"
                     /old_locus_tag="pgaptmp_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4197496.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            10174..10593
                     /locus_tag="ACHKNB_RS20450"
                     /old_locus_tag="pgaptmp_004090"
     CDS             10174..10593
                     /locus_tag="ACHKNB_RS20450"
                     /old_locus_tag="pgaptmp_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HGU4197497.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            10593..10784
                     /locus_tag="ACHKNB_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /pseudo
     CDS             10593..10784
                     /locus_tag="ACHKNB_RS20455"
                     /old_locus_tag="pgaptmp_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2486 a         2682 c         2974 g         2717 t
ORIGIN      
        1 gtcctgcaga gcctgatggg gcataagtcc atcagctcaa cggaggtcta cacgaaggtg
       61 tttgcgctgg atgtggctgc acggcaccgg gtgcagtttt cgatgcctga gtccgatgcg
      121 gtgtctatgc tcaaaagaat tccttaagct ctgacttttt taaatcccct taggatatct
      181 catggatata ttatcggtat ttcatattct gttgacatgc aggaccggta tagagtaaaa
      241 aaaactctat tgagtacctc ttggatatcc gaaaggtgtc ttttgggttc tttatgggta
      301 ttacatgtgg attctggaga tacactatgc ctgttattgc taatacacat cccaaaggtg
      361 gtgtgggtaa gacaacttca tccgtaaaca ttgttggtga aatgaagtct gatacagttg
      421 atctggatac tcataccggg ctttctatca ttctggggct gagacctgaa ggaaaagaaa
      481 tttccgtgaa agtacccaaa acagtggatg agttaatcga aattatgact ccctacaaga
      541 acagcgataa gacgttactt attgactgtg ggggatttga ctcagatctt acccgtactg
      601 cgattgcctt tgcagactgc gttattgtcc cttcaaaaga ctccctgact gagcgtattg
      661 gtttgatgca ttttgatgga gtactggacg aaatcagctc tatcatgggg accgatataa
      721 ctgctcacct gtatctctgt aaagttaatc ctaacaagaa gaaattccct aagcttgatg
      781 caatcctgcc atcgttcaaa catctgaagc tgatgaagag ccgcatttct gctcgtgcag
      841 aatttgagga tgtcattgag actgggatgg gaatcactga gtctgttcat gggcgctatt
      901 cggcaggagg taaggaagtt attgccctta tcgaagaaat caatcatctg attgaaaata
      961 acaaacagta aagtgtatct attgggtatc catatagtat cctttgggct tttaatggat
     1021 atcttttgaa tatctaacaa acatcctggg ttatctcatg agcaaagcga aatttagtgc
     1081 acaggctgaa aaactgaaaa acttggtgga ttctgccaga ttacaaactt cttcttctgc
     1141 cgtaacagca acagtcccgt ctcagccaaa tgaaggtgca gaggaagttt caggtcgtcc
     1201 cgtggccgcc aggacgaagg ctaagaatct taaggctata ccgctgtcgt attttgaagc
     1261 tcatgcggaa cttaaagcga ccagtaaaac atctctggat tttagctcat atattattga
     1321 ggctataaga gagaagcttg aacgtgatgg tgcaataggt caataaacta tcagatacct
     1381 taagggtatc tgatgtgtgt cttttaaatg cgttataggg attccttgtg tacctaaaga
     1441 gtccctatta gacatctttc tgtatatggt gacatgattc gtcactgctt tcctttcctc
     1501 tcagtttcac catttcattc ccttccctta acctgaacca tgatttcaaa gatgttttta
     1561 tattgcattt atgtgttgca tataacattc ataatgtttt tgtatctatg atttctctgg
     1621 tcttaaggtt acatgtgcaa tatacatgca atttattgtg tttttatgtt gctttcaccc
     1681 tttcagaaag accatctttc cattaaaatt gaatatacat tgcatgtatg aagtgtgttt
     1741 gttttttcta tttgtttatg agcaacggag actggttatg aaaatcttta ttgatgatgg
     1801 ttctaccaac attaaacttg cctggttgga ggatggtgac gtgaaaacgt taatcagccc
     1861 taacagcttt aaaccggaat ggtcttttag tttactggat gatgctgccc cggctaatta
     1921 cgagatcgac ggcgagaagt tttcatttga tccattaagt gctgatgctg tcgtaacgac
     1981 tgaaacacgc tatcagtaca gtgatgtgaa tgttgtggcc attcagcatg cgctacagca
     2041 gaccggcctg aaagcgcagc ctgttgatgt catcgtcaca ttgccgattt ctgaatacct
     2101 tgacgccaac aatcagaaaa ataagcaaaa cattgaaaga aagaaaaaaa atgtgatgcg
     2161 tgaagtacgg gtgcaggggg gggatgcatt tgttattcgc agtgtttccg ttcttcctga
     2221 aagtattccc gctggtttca gtgttcttgc ggggcttgag gatgatgaat cccttttgat
     2281 cgtagatctc ggtggtacaa ctcttgatgt gtcgcatgtt cgtagcaaaa tgacagggat
     2341 tacaaaaacc tggtgtgatc cgaatatcgg cgtgtccctg attacatccg gggttaagga
     2401 acagatggct gtacatgcta atacaagagt gagctcattc caggctgaca atatcattgt
     2461 gcaccgtaat gaacctgact acctttcccg tcgcatttat aatgcggaac agcgtgagtc
     2521 tatcattaat gttatcaatg aacggcagaa gttgcttatt aaacgagtta atgatgtcat
     2581 cagccgtttc accgactata cacatgtaat gtgtgttggt ggtggggctg aaattgtggc
     2641 cgaggctgtg aagaacctga ctaaggttcc tgatgagcgc ttttatctga gctcatcccc
     2701 gcagtttgat ttagtcatgg gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg
     2761 aaaataaatc cagacgctgc tcatttgaac ttttcccgga tgaacggaca ggagataaga
     2821 tagcagatga gctcattgca aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga
     2881 tgttagttac tggtgcggcg tttgcagcta tagataaacg tcttcctttg ctgatatcag
     2941 agttactgac agaaaatacg actctggatg atattaataa ggtgatttcc agcgtaatac
     3001 caggtgcttt ttcggttgaa aagaaattgc ttgaattact ggaaaaacaa tctggtctcc
     3061 atacgtctgt ggattgcagt actcctctta cagagcagag tctgtcgaga aatgacggtg
     3121 aagaccagac cagaagaaac gctgaaaata tgttcggaga tgactgaact aacccagcct
     3181 ggccagggga atatctcgcc atttagttgt ataacactga ctcaacattt cccgtttcat
     3241 ttgccattca ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt
     3301 cagttcatcg agtgttttca ttaacttttc gctgttttta tggggctgga tctcatcaaa
     3361 taatcccggc tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat
     3421 atagcggtac ccttcacgcc agacatggtt taaggctctg catgcggcgg caataatgtc
     3481 ccggctgtcc tgtgtgggca atggaagctt ttccacagcg gcattgctgt aacagggttc
     3541 ttttactgca aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgctc
     3601 cccacgtagt ttctctgcag cccgctctgc atactgaaca acagcctggt gcatggcatc
     3661 tttgtctgtg attcgttcac caaaactgcg actacagaca atctgctgtt ttgccggtgg
     3721 tgcttcttcc agggatatgc aggactcgcc gttgagttcg cgtaccgtac gctcaagaat
     3781 gacgctgaag tttttccgga tgaatgccgt gttagcctgc gtcagctgca gtgctgtgtt
     3841 aatacccagc gcattcagct tttccgtcag tctgcgtcct actccccaga cttcaccaac
     3901 tggctgcagc cccagtagct tcaagatccg attacggttt tctgccgtca gcgcgaccac
     3961 accggaaaac tgtggccatt gctttgttgc ccactgtgca cttttagcca gcgtttttgt
     4021 aggtgcaatg cccaccccca tggtgagtcc tgtccagctc tttacctgtt ccctgagctg
     4081 atgaccaaaa acctccggag agatgcaacg gttcacctcc cgcaaatcaa tgaacatttc
     4141 atcaattgag taggactcaa ctgcgggaga aagcgactcc agaacagcca taacccgttg
     4201 gctcatgctg tggtacagcg cataattgct ggaaaataca tgtattttct tctccaggtg
     4261 catttgtctg acctgaaacc agggctgccc cattctgatg ccaagggctt ttgcctccgg
     4321 gctgcgcgcg atcacacagc catcgttatt gctgagtacg atgaccggtt cgttacgaag
     4381 gtccgggcgg aaaacttttt cacatgaggc gtagaaactg ttgatatcag ccagtgcaaa
     4441 catcagcgta actcccgcgt cctgtgtatc acgtgagtga caacaccaaa aatacagatg
     4501 ttttccggat acagtgtgcg gaactccggg ctgtctgaaa ccggctccag ggccgggcgt
     4561 gggcgcaaca gcagtcgttt gacggtgaac tcaccgtcga tctcagcgat aacgatgtcc
     4621 ccgtgttgtg gtttttcggc cctgtccact accagcagat caccattctg cacgccagcc
     4681 tggttcatcg attcaccgct ggcgcgcaga aagaaggtgg ctgcaggtct gctgatgcaa
     4741 tagctgttca gatccagttc ctgctcagca taatcagtgg caggagaagg aaaaccggcc
     4801 tggcaacgat cggcaaacaa cggtcgcacg taactgtcat cgcctgaagg ggcagccgga
     4861 cggtgataaa cggtactcat gctgcaggca gccagctgtc gtcctgccag atttcttcca
     4921 gcagagacag gatacgttct ttatccttgt cgttgcgggc tcctgtgaca gatacagacg
     4981 gggcgctgcc ctggtctata cgcagccaga tttccgggta gtgtggtttc agacgttgtt
     5041 cgatttcatt ctgtagcgcc tgaagtgtgc ctgatttgag gttttttgtg ctctggcggt
     5101 cgaaaagaat ttcaatgcgg atcatgttct catctccctt ttcttttgct gtatgtatat
     5161 acagcattct aataactgaa tccacacaca gtcaaatgtc attattgtga ctaaaaaacg
     5221 ctcaattctc tgagagtgtg gctatatgcc tgctgtgggc tgctgtgtcc agcttatcca
     5281 cagcattttg tgcacggttc tgtggacaaa atacctggtt acccaggccg tgccggcacg
     5341 ttaaccgggc tgcatccgat gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcact
     5401 tacggtggat gaattatgac ggagacgggc gggcaaccgc ccgtttcttt tccggtaaag
     5461 gatgtcgccg tgctcctctt tttactgcgc cggctgacgt ggcgcggcat aaacgctgcc
     5521 tgtggtcagt gtcccgcgtc cggcgggcac gggacgggcg cttttaccgc tcccggctgg
     5581 cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga tgaacgccct tcaagccgct
     5641 ttcgcggcat agccttgccg tcaaaaagac gacggctgcg gtattccacg gtcggcctga
     5701 cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcgct acaggggcgc
     5761 cctcccggtg ctctctgctg aagcagaagt gagaggagaa ttcccatgcc aaactggtgc
     5821 agtaaccgta tgcatttttc tggtgaaccg gcacagattg ctgagattaa acgactggcc
     5881 agtggtgccg tcacaccgtt ttatcgccgc gccacaaatg aaggtattca gctgtttctg
     5941 gccggaagtg ccggacttct gcagaccact gaagatgtgt ggtttgaacc gtgccccgga
     6001 ctgacggctg ccggacgtgg cgttgtatcg ccggagaata tcgcgttcac ccgctggctg
     6061 acacacctgc agaacggtgt actgctggat gagcaaaact gcctgatgct gcatgaactc
     6121 tggctgcaga gcggtactgg ccagcgtcgc tgggaaggat taccggacga tgtcagggat
     6181 accatcaccg cgcttttcac cgcaaaaaga ggtgactggt gtggcttctg gagtaacgag
     6241 gctgtatcgg tgtggtggaa ccgtctgtgt gacaacgtac tgccggaaaa aaccatgccg
     6301 tttgacctgc tgacggttct gcccacccgc cttgatgttg aagtgaatgg ctttaacggt
     6361 ggtgttctga acggtgttcc ttctgcatat cactggtata ccgaacagta tggcgtgaag
     6421 tggccttgtg ggtatgacct gaatatcagc agtcagggag acaacttcat tcaggttgat
     6481 ttcgacacgc cgtggtgtca gccggaaagc gacgttattg cagaattaag ccgccgtttc
     6541 agctgcacgc tggagcactg gtatgccgaa cagggctgtg atttctgtgg ctggcagttg
     6601 tatgagcgcg gagagctcgt tgatgtgctg tggggggagc tggaatggtc ttccccgaca
     6661 gatgacgatg agctgccgga agtcaccgga cctgcgtgga tagtcgacaa ggtggcgcat
     6721 tatggcggat gaagtatgac ggagacgggc gggttaccgc ccgtttcttt tccggtaaag
     6781 gatgtcgccg tgctcctctt tttactgcgc cggctgacgc ggcgcggcat aaacgctgcc
     6841 tgtggtcagt gtcccgcgtc cggccgggca cgggacgggc gcttttaccg ctcccggctg
     6901 gcgtcggtga cagtgtacgc cagcccgtcg cccttttctg atgaacgccc ttcaagccgc
     6961 tttcgcggca tagccttgcc gtcaaaaaga cgacggctgc ggtattccac ggtcggcctg
     7021 acccgttacc ggacgcggtg aacagcccac aggcagcggg gaacgggcac cacaggggtg
     7081 ccctcctggt gccttctgta agagaaggag tgttgttatg tcccgttttg tcctcggtaa
     7141 ctgcatcgat gttatggccc gtattcctga taacgccatt gatttcatac tgactgaccc
     7201 gccgtacctc gtcggttttc gtgaccgttt cgggcgcacc atcgctggcg ataaaaccga
     7261 tgaatggctg caaccggcct gtaatgaaat gtaccgcgta ctcaaaaaag acgcgctgat
     7321 ggtcagcttc tacggctggc accgtattga ccgctttatg gcggcctgga aacgcgccgg
     7381 attcagcgtg gcagggcacc tcgtgttcac caaaaactac acatccaaat ctgcgtatgt
     7441 cgcgtaccgc cacgaatgcg cttacatcct ggcaaagggc agtccggccc ggccccgtaa
     7501 ccctctgccg gatgtgctgg gctggaaata ttcaggcaat cgtcatcacc cgacggaaaa
     7561 gcccgttacc agcctgcaac cactgattga gagcttcaca catccgaacg cgattgtgct
     7621 ggacccgttt gcaggcagcg gctcaacctg cgtggccgcc cttcagtccg gacgccggta
     7681 tatcggtatc gagctgcttg aacagtatca ccgtgccgga cagcaacgac tggccgccgt
     7741 gcaacgggcc atgcagcagg gggccgcgaa tgatgactgg tttatgccgg aggctgcgta
     7801 aatgaactat gccggacacg aaaaactgcg cgccgaagtg gcggaggtgg ccaatgccat
     7861 gtgcgacctg cgtacaacca tgaatgagat ggagcagcgg tacagcttta atgccgacac
     7921 cctgccggaa cgtctggtgc gtcagacgct gtttcgcgca aaccgcctcc tgatggaggc
     7981 atataccgaa attctggaac tggatgcgtg cttcaaagat tgagaaggag acgagaatgt
     8041 acggaacatg cgaaacgctc tgccgggcgc tggcagcaaa gtatccggga gatacgccgc
     8101 tgatgctggt tatctggtcc ccggaagaaa ttcaggccct cgctgacgga atggatattg
     8161 ccctgaccga tcatgaaatc aggactgtcc tggcgcgcct ggaggacatc ccggaagacc
     8221 agcggactga atccggtatt tcttccggcg tggcgatgga gatcatcaat aacgtgagag
     8281 aaaaccgcca ggtgaccgtt ccggctgaac tgctggcgtc cctgattcag accgctgaac
     8341 aggcattgtg gaaacgtgaa tgggccgccc gggatcatgg cctcgccgtc ccggaatgcg
     8401 tcacccgccg tcaggcggtg gttaatcagg tccgcatcct gctgaaaaac aacacacacg
     8461 aaaacgactg atgttatcgc cgcctccggg cggcgataca gggagttcat gaattatgaa
     8521 tgaaacgctg aatgcactga tttatcgcca cgccagcaac ctgttgctgg cgcagggctg
     8581 gccggaagaa acggatgttg accagcgtaa cccgaagtat ccgggctgga tcagcattta
     8641 cgtgcggctg gatgcgcccc ggctggcgac gttacttatc aaccgtcacg gcggcgtact
     8701 gccgccgctc ctggcctctg ccattcagaa actgaccgga accggggcgg aacttgttct
     8761 gtccggcagt cagtggcagt cgctgccggt acttccggca gacggaacgc aggtgtcttt
     8821 cccgtatgcc ggagaatggc tgaccgaaga cgaaatcagg gccgttcttg atgcggtgca
     8881 cgatgcggta cgcagtgtca gttgccgggt ggcagaagat gcgcggcgta tccgggcggc
     8941 gcttaccaca accggtcaga cgttgctgac ccgccagacg cgccgctttc gcctggttgt
     9001 gaaggaaagc gatcacccct gctggctcga tgaagacgac gaaaacctgc ccgtggtgct
     9061 cgatgccatt ctgaaccggg gagcacgttt ttcctctgtg gaaatgtacc tggtcagtga
     9121 gtgtgtggag catatcctgg ccagtggcct ggtctgtgat gtgctgcgta taccggatga
     9181 accgtcccgt cgctggtttg acagggatat tctgcgggag gttgttctgg aggcacggga
     9241 tgaaatccgc agcatggcgg atgccctggc aaaaatccgg aaatgatgac gctggcgggg
     9301 aaaaatctcc gccttttcct gccggtcccc ggacagagca caaaggatgt cgccgccggc
     9361 tccgctttac ccggccatgc ggggcatggc cttgtgggtt ttcagttatg tggcctcagc
     9421 gtcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc ttctgcgggc gggttgcaag
     9481 gtaaggtgaa ccgctgcggt cacccggtcc ctccttcccg tctgccgtga ttttccggtc
     9541 gttcacccgc tcaggtttcg cggtctcacc ctccaactcc cgtcagccgt gtgcgtcagg
     9601 ctgcggcttc ccttgcgccc ttgcaaccct gcaggcccgg caaagatgga ggcacggcac
     9661 cgcccggtgt cgcggaatat gtgaacaacg gaggaaatcc catgcagtat gcgaaacctg
     9721 tcaccctgaa cgttgaagag tgcgaccgcc tttcttttct gccttacctg tttggcaacg
     9781 attttctgta tgccgaggcg tatgtgtacg cgctggcgca aaaaatgatg ccggaatatc
     9841 agggaggctt ctggcatttc atccgcctgc cggacggtgg cggttacatg atgccggatg
     9901 gcgaccgttt tcatctggtc aacggtgaaa actggtttga ccgcactgtg agtgctgatg
     9961 ccgcaggtat catcctgact tcccttgtga ttaaccgcca gttgtggctg taccacgaca
    10021 gcggtgatgc gggactgaca cacctgtacc ggatgcgcga tgcgcagttg tggagccaca
    10081 ttgaatttca ccccgaatgt aacgcgattt atgcagcact ggactgatta acggacgggg
    10141 cggcgatgcc gcccctgtaa aggagacgag aacatgtact gtactgttaa agaaattatc
    10201 cgcgaagtgc tgaatacaga cgtgccggac agtgaatgtg tttttgccgt ggtgctgacc
    10261 cgcggggatg tgcgccacat agcccgggac tggaacctgt cagacgatga gctggaaacc
    10321 gtcatgcagc ggctggacga tgcttttgta tatggcgcat gtgacagagt tgtttccgat
    10381 attgtcaatg aactgatgga ggaaaagcgc gtaaaccgtc ttgtaacagt tcctgcggtg
    10441 ctgctggaaa aagtgatggt gatggctggt agtgaaattt atcgcctcca tgctgtcggg
    10501 agcgagaacg ggggcgacgg tgacgcattt gtcagggaag aacgtgagat tatgcgggta
    10561 atgcgtcagg cgctggacgg ggagaacggg taatgaatat cagcacggaa acccgcgaaa
    10621 ttctgcgtaa ttaaaaagct gtgattaatg cacgacgtcg tgaaatgggg cagaaaccgc
    10681 tcaccaccgc gcagattgtt gatgaaatct gcgattttgt ggtgaatcag caggctgttt
    10741 tcctgggtgg tcactatatc cttcagggca gcaggcacag gtgatacagg ggcaggcggt
    10801 ggaggtcgcc tgtcagcacg cgcgaaaacc cctggcaggc tggcgcctgc cggaaggga
//
LOCUS       DAZFVO020000161        10682 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFVO020000161 DAZFVO020000000
VERSION     DAZFVO020000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.162"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1217
                     /gene="yghO"
                     /locus_tag="ACHKNB_RS20460"
                     /old_locus_tag="pgaptmp_004092"
     CDS             75..1217
                     /gene="yghO"
                     /locus_tag="ACHKNB_RS20460"
                     /old_locus_tag="pgaptmp_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HGU4197498.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1224..1988)
                     /gene="glcC"
                     /locus_tag="ACHKNB_RS20465"
                     /old_locus_tag="pgaptmp_004093"
     CDS             complement(1224..1988)
                     /gene="glcC"
                     /locus_tag="ACHKNB_RS20465"
                     /old_locus_tag="pgaptmp_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HGU4197499.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2239..3738
                     /gene="glcD"
                     /locus_tag="ACHKNB_RS20470"
                     /old_locus_tag="pgaptmp_004094"
     CDS             2239..3738
                     /gene="glcD"
                     /locus_tag="ACHKNB_RS20470"
                     /old_locus_tag="pgaptmp_004094"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HGU4197500.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3738..4790
                     /gene="glcE"
                     /locus_tag="ACHKNB_RS20475"
                     /old_locus_tag="pgaptmp_004095"
     CDS             3738..4790
                     /gene="glcE"
                     /locus_tag="ACHKNB_RS20475"
                     /old_locus_tag="pgaptmp_004095"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HGU4197501.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4801..6024
                     /gene="glcF"
                     /locus_tag="ACHKNB_RS20480"
                     /old_locus_tag="pgaptmp_004096"
     CDS             4801..6024
                     /gene="glcF"
                     /locus_tag="ACHKNB_RS20480"
                     /old_locus_tag="pgaptmp_004096"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HGU4197502.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6029..6433
                     /locus_tag="ACHKNB_RS20485"
                     /old_locus_tag="pgaptmp_004097"
     CDS             6029..6433
                     /locus_tag="ACHKNB_RS20485"
                     /old_locus_tag="pgaptmp_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HGU4197503.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6455..8626
                     /gene="glcB"
                     /locus_tag="ACHKNB_RS20490"
                     /old_locus_tag="pgaptmp_004098"
     CDS             6455..8626
                     /gene="glcB"
                     /locus_tag="ACHKNB_RS20490"
                     /old_locus_tag="pgaptmp_004098"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HGU4197504.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGQQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8982..10664
                     /gene="glcA"
                     /locus_tag="ACHKNB_RS20495"
                     /old_locus_tag="pgaptmp_004099"
     CDS             8982..10664
                     /gene="glcA"
                     /locus_tag="ACHKNB_RS20495"
                     /old_locus_tag="pgaptmp_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HGU4197505.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2474 a         2704 c         3022 g         2482 t
ORIGIN      
        1 gttgattatt aaggtgtcgt tcgttgctgt tgcaacctgt ttttcaccgt taattatttg
       61 ttatccacag agatatggaa tgcgatctgc tgatgattaa aattgaaaaa gtaattaata
      121 aaaatgacct taaggcattt atcgcttttc cgtcatcact ttatcccgac gatccaaact
      181 ggataccccc tttattcata gaacgcaacg agcatttgtc tgcgaaaaat ccggggacgg
      241 accatatcat ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt gggcgtatta
      301 ccgcgcaaat agataccttg caccgtgagc gttacggtga agataccggt catttcggca
      361 tgattgacgc cattgatgat ccgcaggttt ttgccgcgtt gtttggcgca gcggaagcgt
      421 ggttgaagtc acaaggtgca agtaagatca gcggtccttt cagcctgaat atcaatcagg
      481 aaagcggatt actgattgaa ggttttgaca caccaccctg tgcgatgatg ccacacggca
      541 aaccgtggta tgccgcgcat attgaacaac tgggttatca caaaggcatt gatttactgg
      601 cgtggtggat gcagcgaacc gatctcactt tctctccggc gctaaaaaaa ctgatggatc
      661 aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc gaagaaatgc
      721 agatcctgcg tgagattttc aactctggct ggcaacacaa ctggggattt gtgccgttta
      781 ccgaacatga attcgcgacc atgggcgatc aacttaaata tctggtgccg gacgatatga
      841 tctatatcgc tgagattgat tctgcaccct gcgcgtttat tgtcggcttg ccgaacatca
      901 acgaggcgat tgccgatctg aacggatcgc tctttccctt cggctgggca aaattgctgt
      961 ggcgtttgaa agtcagcggt gtgcgaactg cgcgagtacc gctgatgggc gtgcgcgacg
     1021 agtatcagtt cagccgcatc ggcccggtga ttgcgctgtt attgattgaa gccttacgcg
     1081 atccgtttgc ccgccggaag atcgatgcgc tggagatgtc atggatcctc gaaaccaata
     1141 caggcatgaa taacatgctg gaacgcattg gggcggagcc gtacaagcgt taccgattgt
     1201 atgaaaagca gatttaatac cagctaactc aggttcatct ccagcggcac cgccgagcga
     1261 atcaaatggt ggccttccag ctcgatatcg tggagattct ttttcacggt ccgcacatga
     1321 tcgcgtgctg cgcgctgggc gacgtgcggc agccgctgca acaccgcgtt gtagatcctc
     1381 gcatgctggc gatcgatctg ctttttttgc tgtggtcgat ggtagagatt atttacgctg
     1441 gcaaacactg aattaaacat cagatcggtc aatgattgca gcgtaaacac cagcacctga
     1501 ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat ccaactgcgc atgttcgatc
     1561 agcgaaatct ctttgttgtt ctcactggcg gcgagcattt tttcataaca gcgggttatc
     1621 acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc ttgccgattc gccctccagt
     1681 aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct gcgtactgaa cagatggatc
     1741 agcgggctgg tgtcctgcac ccgattaagc cgtgcgacac gagaatcgcg accctgcgcc
     1801 gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt cacgcagtgc ggagcgtgag
     1861 aagccgagct tttcacacag tcgacgttcc gagggaagtg gctgaccgac cttcagtacg
     1921 ccgtcgataa ttaaccgttc gatactctct gcaaccactt cgcaaatagg gcgacgttca
     1981 tctttcattc ccggacctcg tgcacaggta ggaccaattt ttctgttctt aactcgcaaa
     2041 acacgcacat cacgtaagtg attatgttac atcaatttaa cattgagtta accaaggcaa
     2101 ggtcacagag ctggaaaaaa aatggtctga ccggtagcta aagagataga cgaaaacgaa
     2161 aagcccgctt aataactgtt cacagaagca gcgcgcaaaa atcagctgcc acacaacaca
     2221 acaaagcgaa gcctactcat gagcatcttg tacgaagagc gtcttgatgg cgctttaccc
     2281 gatgtcgacc gcacatcggt actgatggca ctgcgtgagc atgtccctgg acttgagatc
     2341 ctgcataccg atgaggagat cattccttac gagtgtgacg ggttgagcgc gtatcgcacg
     2401 cgtccattac tggttgttct gcctaagcaa atggaacagg tgacagcgat tcttgctgtc
     2461 tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag gcaccgggct ttctggtggc
     2521 gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc gctttaaaga gatcctcgac
     2581 attaaccccg ttggtcgccg cgcgcgcgtg cagccaggcg tgcgtaacct ggcgatctcc
     2641 caggccgttg caccgcataa tctctactac gcaccggacc cttcctcaca aatcgcctgt
     2701 tccattggcg gcaacgtggc agaaaatgcc ggcggcgtcc actgcctgaa atatggtctg
     2761 accgtacata acctgctgaa aattgaagtg caaacgctgg acggcgaggc gctgacgctg
     2821 ggatcggacg cgctggattc acctggtttt gacctgctgg cgctgttcac cggatcggaa
     2881 ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc tgccgaagcc gcccgtggcg
     2941 cgggttctgt tagccagctt tgactcggta gaaaaagccg gacttgcagt tggtgacatc
     3001 atcgccaatg gcattatccc cggcgggctg gagatgatgg ataacctgtc gatccgcgcg
     3061 gcggaagatt ttattcatgc cggttatccc gtcgacgccg aagcgatttt gttatgcgag
     3121 ctggacggcg tggagtctga cgtacaggaa gactgcgagc gggttaacga catcttgttg
     3181 aaagcgggcg cgactgacgt ccgtctggca caggacgaag cagagcgcgt acgtttctgg
     3241 gccggtcgca aaaatgcgtt cccggcggta ggacgtatct ccccggatta ctactgcatg
     3301 gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg aaggcattgc ccgtttatcg
     3361 cagcaatatg atttacgtgt tgccaacgtc tttcatgccg gagatggcaa catgcacccg
     3421 ttaatccttt tcgatgccaa cgaacccggt gaatttgccc gcgcggaaga gctgggtggg
     3481 aagatcctcg aactctgcgt tgaagttggc ggcagcatca gtggggaaca tggcatcggg
     3541 cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg atgaaatcac gaccttccat
     3601 gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc ctgggaaaaa cattcccacg
     3661 ctacaccgct gtgctgaatt tggtgccatg catgtgcatc acggtcattt acctttccct
     3721 gaactggagc gtttctgatg ctacgcgagt gtgattacag ccaggcgctg ctggagcagg
     3781 tgaatcaggc aattagcgat aaaacgccgc tggtgattca gggcagcaat agcaaagcct
     3841 ttttaggtcg ccctgtcacc gggcaaacgc tggatgtgcg ttgtcatcgc ggcattgtta
     3901 attacgaccc gaccgagctg gtgataaccg cgcgtgccgg aacgccgctg gtggcgattg
     3961 aagcggcgct ggagagcgcc gggcaaatgc tcccctgcga gccgccgcat tatggtgaag
     4021 aagccacctg gggcgggatg gtcgcctgcg ggctggcggg gccgcgtcgc ccgtggagcg
     4081 gttcggtccg cgattttgtc ctcggcacgc gcatcattac cggcgctgga aaacatctgc
     4141 gttttggtgg cgaagtgatg aaaaacgttg ccggatacga tctctcacgg ttaatggccg
     4201 gaagctacgg ttgtcttggc gtgctcactg aaatctcgat gaaagtgtta ccgcgaccgc
     4261 gcgcctccct gagcctgcgt cgggaaatca gcctgcaaga agccattaac gaaatcgccc
     4321 agtggcaact ccagccgcta cccattagtg gcttatgtta cttcgacaat gcgttgtgga
     4381 tccgccttga gggcggcgaa ggatcggtaa aagcggcgcg tgaactgctg gggggcgaag
     4441 aggttgcggg tcagttctgg cagcaattgc gtgaacaaca actgccgttc ttctcgttac
     4501 caggtacctt atggcgcatt tcattaccca gcgatgcgcc gatgatggat ttacccggcg
     4561 agcaactgat cgactggggc ggagcgttac gctggctgaa atcgacagcc gaggacaatc
     4621 aaatccatcg catcgcccgc aacgctggcg gtcatgcgac ccgctttagt gccggagatg
     4681 gtggctttgc cccgctatcg gctcctttat tccgctatca ccagcagctt aaacagcagc
     4741 tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc ggaactttga ggagcaggct
     4801 atgcaaaccc aattaactga agagatgcgg cagaacgcgc gcgcgctgga agccgacagc
     4861 atcctgcgcg cctgtgttca ctgcggattt tgtaccgcaa cctgcccaac ctatcagctt
     4921 ctgggcgatg aactggacgg gccgcgcggg cgcatctatc tgattaaaca ggtgctggaa
     4981 ggcaacgaag tcacgcttaa aacacaggag catctcgatc gctgcctcac ttgccgcaat
     5041 tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt tgctggatat cgggcgtgat
     5101 attgtcgagc agaaagtgaa acgcccactg ccggagcgaa tgctgcgcga aggattgcgc
     5161 caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc aggtagggct ggtgctgcga
     5221 ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg aaacggtgaa agctaaaccg
     5281 cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg aaggctgcgc ccagcctacg
     5341 ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg atcgtctggg gatcagcgtc
     5401 atgtcagcta acgaagcagg ctgttgtggc gcggtggact atcatcttaa tgcgcaggag
     5461 aaagggctgg cacgggcgcg caataatatt gatgcctggt ggcccgcgat tgaagcaggt
     5521 gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt ttgtcaaaga gtacgggcag
     5581 atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc aagtcagtga actggcggtc
     5641 gatttagtcg agcttctgcg cgaggaaccg ctggaaaaac tggcagttcg cggcgataaa
     5701 aaactggcct tccactgccc gtgtacccta caacatgcgc aaaagctgaa cggcgaagtg
     5761 gaaaaagtgt tgcttcgtct tggatttacc ttaacggacg ttcccgacag ccatctgtgc
     5821 tgcggttcag cgggaacata tgcgttaacg catcccgatc tggcacgcca gctgcgggat
     5881 aacaaaatga atgcgctgga aagcggcaaa ccggaaatga tcgtcaccgc caacattggt
     5941 tgccagacgc atctggcgag cgccggtcgt acctctgtgc gtcactggat tgaaattgta
     6001 gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa gtcattctta gccagcaaat
     6061 ggcgagtgca attattgccg caggtcagga agaggcgcag aaaaataact ggtctgtttc
     6121 cattgctgtt gccgatgacg gcggtcatct gctggcgtta agtcgcatgg acgattgcgc
     6181 gccgattgcg gcttacatct cccaggagaa agcgcgtacc gccgcgctgg ggcgtcgtga
     6241 aactaagggc tatgaagaga tggtgaacaa cggacgtacc gcgttcgtga ctgcgccgtt
     6301 actgacgtcg ctggaaggtg gcgtaccggt tgttgttgat gggcaaatta ttggtgccgt
     6361 gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc gcgaaagcgg cagcagcggt
     6421 gttggcgaaa taagcgaaaa cgaggagata aacaatgagt caaaccataa cccagggccg
     6481 tttacgcatt gacgccaatt ttaaacgttt tgtggatgaa gaagttttac cgggaacagg
     6541 gctggacgct gcggcgttct ggcgcaattt tgatgaaatc gttcacgatc tggcaccaga
     6601 aaatcgtcag ttgctggcag aacgcgatcg cattcaggcg gcgcttgatg agtggcatcg
     6661 cagcaatccg gggccggtaa aagataaagc ggcctataaa tctttcctgc gtgaactggg
     6721 ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc acgggcattg acagcgaaat
     6781 caccagtcag gcggggccac agctggtggt tccggcaatg aacgcccgct acgcgctgaa
     6841 cgcggcgaac gctcgctggg gctcactgta cgatgcgtta tacggcagcg acatcatccc
     6901 gcaggaaggg gcgatggtca gcggctacga tccgcaacgc ggtgagcagg ttatcgcctg
     6961 ggttcggcgt ttcctcgatg aatctctacc gctggaaaac ggcagctatc aggatgtggt
     7021 ggcgtttaag gtggtcgata aacaattacg catccagttg aaaaatggta aagaaaccac
     7081 gttacgtacc ccggcgcagt ttgtcggtta ccgtggcgat gccgctgcgc cgacctgcat
     7141 tttgctgaaa aataacggcc tgcatattga actgcaaatt gatgccaacg ggcggattgg
     7201 caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa gcggccatca gtaccattct
     7261 cgactgcgaa gattcggtcg cggcggttga tgcggaagat aaaatcctgc tgtaccgcaa
     7321 cctgctgggc ctgatgcagg ggactctgca agagaaaatg gagaaaaacg gtcagcaaat
     7381 cgtgcgtaaa ctgaatgacg atcgtcatta caccgccgcc gatggctctg aaatttctct
     7441 gcacggacgc tcgctgctgt ttatccgcaa cgtgggtcat ttgatgacca ttcctgtgat
     7501 ttgggacagc gaaggcaatg aaatcccgga aggcattctt gatggcgtca tgactggcgc
     7561 gattgccctc tatgatttaa aagtgcagaa aaactcgcgc actggcagcg tctatattgt
     7621 gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc aacaaactgt ttacccgcat
     7681 tgagacaatg ctcggtatgg caccgaatac cctgaaaatg ggcattatgg atgaagaacg
     7741 tcggacctcg ctgaacttgc gtagctgtat cgctcaggcg cgcaaccgcg tggcgttcat
     7801 caataccggt ttcctcgacc gtaccggcga tgaaatgcat tcggtgatgg aagctggccc
     7861 gatgctgcgt aaaaaccaga tgaaatcgac gccgtggatc aaagcctacg agcgtaataa
     7921 cgtgctttcc ggtctgttct gtgggctgcg cggtaaagcg caaattggta aaggcatgtg
     7981 ggcaatgccg gacctgatgg cagacatgta cagccagaag ggcgaccaac tgcgtgccgg
     8041 ggcaaacaca gcctgggttc cgtcaccaac cgctgctacg ctccatgcgc tgcactacca
     8101 ccaaaccaac gtacagagcg tgcaagccaa cattgcccag accgagttca atgctgaatt
     8161 tgaaccgctg ctggacgatc tgctgactat tccggtggct gaaaacgcta actggtcggt
     8221 ggaagagatc caacaagagc tggataacaa cgtgcagggg attctggggt acgtggtgcg
     8281 ctgggtggag caggggattg gttgttcaaa agtgccggat attcacaatg tggcgctgat
     8341 ggaagaccgc gcaacgctgc gtatctccag ccagcatatc gccaactggt tacgtcacgg
     8401 tattctgacc aaagaacagg tgcaggcgtc gctggagaat atggcgaaag tggttgatca
     8461 gcaaaacgct ggcgatccgg cttatcgtcc gatggcgggg aatttcgcta actcgtgtgc
     8521 ttttaaagct gccagcgatt taatcttcct cggcgtgaaa cagccaaacg gctataccga
     8581 accgttatta cacgcctggc gtttacgcga aaaagaaagt cattaatagc gtcaaaaaaa
     8641 gccccggcaa gttttattgt cggggctatg atagtaactg cggtagtaca tttatcccgg
     8701 aaataaccgg agcgattatc agggtttaac aatattaaat acccgtagcg cattactagc
     8761 gggcctataa caacaatatg gacatcttaa cgatactctg cgcctgcgtg tgcagaacag
     8821 aaacccttac ccgatatact tgttatgcca gaggtagcaa ttgcgttagc ggcgtactgt
     8881 tgctgtaaag tataacgcct atattttaca gttcagtcct cttttgctta ttacaacctt
     8941 aagtatatca agcatataaa gataataaga gactgaacaa tatggttacc tggacccaaa
     9001 tgtatatgcc gatgggagga ctggggctat ccgctctggt cgccctgatc ccgataatat
     9061 tcttcttcgt tgcactcgcg gtattacgtc tgaaaggaca tgtcgctgga gcaataaccc
     9121 ttatattatc tatcctgatt gcaatattcg cctttaaaat gccgattgat atggcttttg
     9181 ctgctgcggg ctatggcttt atttatggat tatggcctat tgcgtggatt attgtcgcgg
     9241 cggtgttcct ctataaatta accgttgcca gcggtcagtt cgatattatc cgcagctcgg
     9301 ttatctccat caccgacgat cagcgtttgc aggtgttact gattggtttc tcctttggtg
     9361 cgttactgga aggagcggct ggctttggtg cgccggtggc gattaccggt gcgctgctgg
     9421 tgggcctggg cttcaaaccg ttatacgcgg cggggctgtg tctgattgcc aacactgcgc
     9481 cagtggcgtt tggtgcgttg ggcgtgccga ttctggtcgc cggtcaggta acgggaatcg
     9541 atccgttcca cattggcgca atggcgggac gtcagttacc gttcctgtcg gttcttgtgc
     9601 cgttctggct ggtagcaatg atggacggct ggaaaggggt gaaagagacg tggccagcgg
     9661 cgctggttgc tgggggaagc tttgctgtca ctcagttctt tacctctaac tatattggtc
     9721 cggaactgcc ggatattact tcggcgctgg tgagtatcgt ctcactcgct ttattcctta
     9781 aagtctggcg gccgaaaaat accgaaacgg caatcagcat ggggcaatcc gcaggtgcga
     9841 tggtggtaaa taagccatct tctggcggtc ccgtgccttc agaatatagt ctggggcaaa
     9901 tcattcgtgc gtggtcaccg tttttaatct taacggtgct ggtcaccatc tggaccatga
     9961 agccgtttaa agcgttattt gctccgggcg gcgcgtttta ttcactggtg attaatttcc
    10021 agatccctca tttgcatcaa caagtgttga aagcggcacc cattgtcgcc caaccaacgc
    10081 caatggatgc ggtgtttaaa ttcgaccctc tctcggctgg cggcaccgct atttttattg
    10141 cggcgattat ctctatcttc atcctcggcg tgggcatcaa gaaagggatt ggcgtctttg
    10201 ccgaaacgct aattagtctg aagtggccga tattgtcgat tggcatggtg ctggcgttcg
    10261 ccttcgtcac caactattcc ggcatgtcca ccacgttggc gctggtactg gcaggtacag
    10321 gcgtgatgtt cccgtttttc tcaccgttcc tcggctggct gggggtattc cttaccggct
    10381 cggacacttc ttcaaacgcc ctgtttggtt cactgcaatc gaccacggcg cagcaaatca
    10441 acgtctctga cactctgctg gtggcagcaa ataccagcgg cggcgtaact ggcaagatga
    10501 tctccccgca atctatcgcc gtggcctgcg ccgcgacggg catggtgggc cgagaatctg
    10561 aactattccg ctacaccgtg aagcacagtc tgatttttgc cagcgttatc ggcgttatca
    10621 cgttgttgca ggcgtatgtg tttactggaa tgttggtctc gtaaatcaat aatgccggat
    10681 ga
//
LOCUS       DAZFVO020000162        10621 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFVO020000162 DAZFVO020000000
VERSION     DAZFVO020000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.163"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..5957
                     /locus_tag="ACHKNB_RS20500"
                     /old_locus_tag="pgaptmp_004100"
     CDS             <1..5957
                     /locus_tag="ACHKNB_RS20500"
                     /old_locus_tag="pgaptmp_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HGU4197506.1"
                     /translation="VSIDNVKPGVTTDWKETADGVYKATYTAYTRGSGLTAKLLMQNW
                     NEDLHTAGFIIDANPQSAKIATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVT
                     FAVLSGSATSFNNQNTAKTDVNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGD
                     SSTAQVELQKSKNEVVADGNDSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEV
                     NSHDGIATATLTSLKNGDYRVTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVT
                     NTAPLHMTATLQDKNGNPLKDKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTG
                     TLAGTHMITARLANSNVSDTQPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVK
                     DPFDNVVKNLSVVFRTSPADTQLSLKALNTNENGIAEVTLKGTVLGVHTAEAILLNGK
                     SDTKIVNIVPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVAGITVNFTMP
                     QDVAANFTLENNGIAITQANGEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADK
                     DSAVVVLKTSKAEIIGNGVDETTLTATVKDPFDNVVKDLPVTFSTNPADTQLSQSTSN
                     TNDSGVAEVTLKDTVLGVHTVEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTN
                     NSDSVQLTAMVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKG
                     KKAGTHTVTATLGNNNTSDSQPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVK
                     DQFDNEVNNLPVTFSSASSGLTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNG
                     ASDNKTVHFIGDTAAAKIIELTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTV
                     NFTSRTNSAEMTNGGQAVTNEQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLST
                     SINVNADASTAHLTLLQALFDTVSAGDTTNLYIEVKDNYGNGVPQQEVTLRVSPSEGV
                     TPSNNAIYTTNHDGNFYTSFTATKAGVYQVTATLENGDSMQQTVTYVLNVANAEITLA
                     ASKDPVIADNNDLTTLTATVADTEGNAIANTEVTFTLPEDVRANFTLSDGGKAITDTE
                     GKAKVTLKGIKAGAHTVTASMAGSKSGQLVVNFTADTLTAQVNLNVTEDNFIANNIGM
                     TKLQATVTDGNGNPFANEAVTFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSG
                     TYPVTVSVINYGVSDTKQVTLIADAGTATLASLTSVYSFVVSTTEGATMTASVTDANG
                     NPVEGIKVNFRGTSVTLSSTSVETDDQGFAEILVTSTEVGLKTVSASLADKPTEVISR
                     LLNAKADINSATITSLEIPEGQLMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHM
                     TISQNIVSTDTHGIAEVSMTPERNGSYMVKASLANGASLEKQLEAIDEKLTLTASSPL
                     IGVYAPTGTTLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTS
                     NKVGTYTVTASFHNGVTIQTQTTVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKAT
                     VEDGSGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTA
                     GGMQTVDITLVAGPADASQSVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGME
                     FVQSGTNVPYMKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQF
                     TRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASW
                     VDVDATGKVTFKNVGSNWERITATPKSGGPSYVYEIRVKSWWVNSGDAFMIYSLAENF
                     CSSNGYTLPRADHLNHSRSRGIGSLYSEWGDMGHYTTDAGFQSNMYWSSSPANSSEQY
                     VVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(6219..7271)
                     /locus_tag="ACHKNB_RS20505"
                     /old_locus_tag="pgaptmp_004101"
     CDS             complement(6219..7271)
                     /locus_tag="ACHKNB_RS20505"
                     /old_locus_tag="pgaptmp_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HGU4197507.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            7586..8902
                     /gene="shiA"
                     /locus_tag="ACHKNB_RS20510"
                     /old_locus_tag="pgaptmp_004102"
     CDS             7586..8902
                     /gene="shiA"
                     /locus_tag="ACHKNB_RS20510"
                     /old_locus_tag="pgaptmp_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HGU4197508.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            9004..10458
                     /gene="amn"
                     /locus_tag="ACHKNB_RS20515"
                     /old_locus_tag="pgaptmp_004103"
     CDS             9004..10458
                     /gene="amn"
                     /locus_tag="ACHKNB_RS20515"
                     /old_locus_tag="pgaptmp_004103"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HGU4197509.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         2889 a         2563 c         2522 g         2647 t
ORIGIN      
        1 cagtcagcat cgacaacgtg aaaccaggag tcactacaga ctggaaagaa accgcagatg
       61 gcgtctataa ggcgacctat accgcctata ccagaggcag tgggcttact gcgaagctat
      121 taatgcaaaa ctggaatgaa gatttacata ccgctggatt tatcatcgac gccaacccgc
      181 agtcagcgaa aattgcgaca ttatctgcca gcaataatgg tgtgctcgcc aatgagaatg
      241 cagcaaacac cgtctcggtc aatgtcgctg atgaaggaag caacccaatc aatgatcata
      301 ccgtcacgtt tgcggtatta agcggatcgg caacttcctt caacaatcaa aacaccgcaa
      361 aaacggatgt taatggtctg gcgacttttg atctgaaaag tagtaagcag gaagacaaca
      421 cggttgaagt tacccttgaa aatggcgtga aacaaacgtt aatcgtcagt tttgtcggcg
      481 actcgagtac cgcgcaggtt gagctgcaga agtcgaaaaa tgaagtggtc gctgacggca
      541 atgatagcgc cacaatgacc gcgacagtcc gggatgcaaa aggcaacctg ctcaatgacg
      601 tcaaggtcac tttcaatgtt aattcagcag aggcgaaact gagccaaaca gaagtgaata
      661 gccacgacgg gatcgccaca gctacgctga ccagtttgaa aaatggtgat tatagggtta
      721 cggcctctgt gagctctggt tcccaggcta atcaacaggt gaattttatc ggtgatcaaa
      781 gtactgctgc cctgaccctc agtgtgcctt caggtgatat caccgtcacc aacacagctc
      841 cgctacatat gactgcaacc ttgcaggata aaaatggcaa cccattaaaa gacaaagaaa
      901 tcaccttctc tgtgccaaac gacgtcgcaa gtcggttctc gattagcaac agcggaaaag
      961 gcatgacgga tagcaacggg actgcaatcg cctccctgac cggcacgtta gcgggcacgc
     1021 atatgatcac ggctcgtctg gctaacagca atgtcagcga tacacagcca atgacgtttg
     1081 tggcggataa agacagagcg gttgtcgttc tgcaaacatc gaaagcggaa atcattggga
     1141 atggcgtgga tgagacaact ctgacagcaa cagtgaaaga tccttttgat aacgtggtta
     1201 aaaatctgtc agtagtcttc cgcacctccc ccgcagacac gcaactgagt ctgaaggcgc
     1261 ttaatactaa tgagaacggt attgctgaag ttacccttaa aggcacggtt ttgggtgttc
     1321 atacagccga agccatactg cttaacggta aaagcgatac gaaaatcgtc aatattgtgc
     1381 ccgataccag caacgcgcag gtcaccctga acatcccggc tcaacaggtg gtgacgaata
     1441 acagcgacag cgtacagctg acggcgatgg tgaaagatcc gtcgaatcat ccggtggcgg
     1501 gaataacggt gaacttcacc atgccacagg acgttgcggc aaactttacc cttgaaaata
     1561 acggtattgc catcactcag gccaatggcg aagcgcatgt cacgctcaaa ggtaaaaaag
     1621 cgggcatgca tacggttacc gcaacgttgg gtaacaataa tgccagcgat gcgcaaccag
     1681 tcacattcgt ggcggataag gacagcgcag ttgtcgttct gaaaacatcg aaagcggaaa
     1741 tcattgggaa tggcgtggat gagacgactc tgacggcaac agtgaaagat ccttttgata
     1801 acgtagtaaa agatctaccg gtcaccttca gtaccaaccc ggcagatact caacttagtc
     1861 agagcacaag caatactaac gacagtggtg ttgctgaagt tacccttaag gacacggttt
     1921 tgggtgttca tacagtcgaa gccacgctgc ttaacggcaa tggctatacg acgacagtca
     1981 atattgcgcc cgatgccagc aacgcgcagg tcaccctgaa catccctgct caacaggtgg
     2041 tgacgaataa cagtgacagc gtgcagctta cggcgatggt gaaagatccg tcgaatcatc
     2101 cggtggcggg gataacggtc aacttcacca tgccacagga cgttgcggca aactttaccc
     2161 tcgaaaataa cggcattgcc atcacccaag ccaatgggga agcgcatgtc acgctgaaag
     2221 gtaaaaaagc gggtacgcat acggttaccg caacgctggg taataacaat accagtgatt
     2281 cgcagccggt aacgtttgtg gcggacaaaa cctcggctca ggttgtcctg cagatgtcaa
     2341 aagatgagat cacaggtaat ggcgtcgata acgcaacgct aactgcaacg gttaaagatc
     2401 agttcgacaa tgaggtgaat aatcttccgg taacattcag ctcagcctct tcaggactca
     2461 ccctgacccc gggagtaagt aataccaatg agtctggcat cgcgcaggcc actctcgcag
     2521 gcgttgcctt tggtgagcag acggtcactg catcactggc taataatggt gccagcgaca
     2581 acaaaactgt gcattttatt ggcgacacag cggcggcaaa aattatcgag ttgacgcctg
     2641 tcccagacag cataatcgcc ggtaccccgc agaacagctc cggcagcgtc atcaccgcca
     2701 cagtcgttga taataatggc tttccggtga aaggtgtgac tgtgaacttc accagcagaa
     2761 caaactctgc cgaaatgacg aatggcggcc aagccgtaac gaacgaacag ggtaaggcta
     2821 ccgtcactta taccaatacc cgctcctcga tagaatcagg agcgagaccg gataccgttg
     2881 aggccagtct ggaaaatggt agctccacgc ttagcacatc aattaatgtc aacgctgatg
     2941 cgtctacggc acatctcacc ttgctacagg cactttttga tacagtctcc gcaggcgaca
     3001 ctaccaatct gtatattgag gtgaaggata attacggcaa cggtgtaccc cagcaggagg
     3061 taaccctcag agtatcacca agtgaaggcg tgacccccag taataacgct atatatacta
     3121 ccaaccacga cggcaatttt tacacaagct ttaccgctac aaaagccggg gtttatcaag
     3181 tgacggcaac cctcgaaaat ggcgattcga tgcaacaaac agtgacctat gtgctgaacg
     3241 tcgcgaatgc cgaaatcacg ctggcagcct cgaaggatcc ggtgattgcc gacaataacg
     3301 atctcacgac actaacagca acagtcgctg atacagaggg caatgcgata gccaacactg
     3361 aagtaacatt tactctgccg gaagatgtga gggcgaactt cacgctgagc gatggcggta
     3421 aagcgattac tgatactgaa ggcaaagcga aagtcacgct gaaaggtata aaagcaggcg
     3481 ctcatactgt tacagcatcg atggctggca gtaagagtgg acaactggtg gtgaacttta
     3541 ctgcggatac gctcactgcg caggttaatc ttaacgttac cgaggacaat ttcatcgcca
     3601 ataacattgg gatgaccaag ctgcaggcaa cagtgactga tggaaacggc aacccattcg
     3661 ccaatgaggc ggtgacattc acgctaccgg cagatgtgag cgcaagcttc actctcggac
     3721 aaggcggttc cgccattact gatatcaacg gcaaggctga agttacactg agcggtacca
     3781 aatccggcac ctatcccgtg acagttagcg tgatcaatta tggtgtcagt gatacgaaac
     3841 aggtgacttt gattgccgat gctggtaccg caacactagc ctccttaacc tctgtatact
     3901 cattcgtcgt cagcacgacc gagggcgcga ccatgactgc aagcgtcact gacgctaacg
     3961 gcaacccggt agaaggcata aaagttaatt tccgcggaac ctccgtcacg ctaagcagca
     4021 ccagcgttga aacggatgat cagggtttcg ctgaaattct tgtgacaagc accgaagtcg
     4081 gactgaaaac agtttcagcc tctctggcag ataaacctac tgaagtcata tcgcgattac
     4141 tgaatgcaaa agcagatatt aattctgcaa cgattaccag tctggagata cctgaaggtc
     4201 agctaatggt cgcacaagac gtagcagtta aagctcacgt caacgaccag tttggcaacc
     4261 cgattcttaa tgaatctgta acattcagtg cagagccacc agagcacatg accatcagcc
     4321 aaaatattgt ctctactgat acgcatggta tagccgaggt ctccatgacg cccgaaagaa
     4381 acggttcgta tatggtgaaa gcatccctgg cgaatggagc ctcacttgag aaacaactgg
     4441 aggctattga tgaaaaactg acactcacgg cgtccagtcc gcttatcggt gtctatgccc
     4501 ctacaggcac tactctgacg gcaacgctaa cctctgcaaa tggcactcca gtggagggtc
     4561 aggtcatcaa ctttagcgta acgccagaag gggcgacgtt aagtggcgga aaagtgagaa
     4621 ctaactcttc aggtcaggct ccggtcgttc tgaccagcaa taaagtcggt acatatacgg
     4681 tgactgcatc gttccataac ggcgtaacaa tacagacaca gacaaccgtg aaagtcactg
     4741 gcaactcaag caccgcacat gttgctagct ttatcgctga tccatcgact atcgccgcca
     4801 ccaacagtga tttaagtacc ttaaaggcaa cggttgagga tggcagtggt aacctgatcg
     4861 aaggtctcac tgtgtacttc gccttaaaaa gcggctctgc cacattaacg tcattaacag
     4921 cggtgaccga tcaaaacgga atcgcgacaa caagcgtgaa aggagcgatg acaggtagcg
     4981 tcacggtaag cgcagtcacg accgctggtg gaatgcaaac agtagatata acgctggtgg
     5041 caggcccggc agacgcctcg cagtccgtcc ttaagaacaa tcggtcctca ctgaaagggg
     5101 actttaccga tagtgctgag ctacatcttg ttttgcacga tatatcaggc aatccgatca
     5161 aagtttctga agggatggaa tttgtgcaat caggtactaa cgtgccctat atgaaaatta
     5221 gcgcaattga ttacagtcta aatatcaacg gtgattacaa agccactgtt acaggcggcg
     5281 gagagggtat cgcaacgctg atccctgtat tgaatggtgt tcatcaagct ggtctgagta
     5341 ccacaataca attcactcgc gcagaagaca aaataatgag cggtacagta tcagtcaatg
     5401 gtactgacct accgacaact acattccctt cgcaggggtt caccggggcg tattatcagt
     5461 tgaataatga caactttgcc ccaggaaaaa cggcggctga ttatgagttt tcaagctctg
     5521 cctcctgggt cgatgttgat gctaccggta aagtgacatt taaaaatgtc ggcagcaatt
     5581 gggaaaggat tacggcgacg ccaaaatcag gaggccctag ctatgtatac gaaatccgtg
     5641 tgaagagttg gtgggtgaac tccggcgatg ctttcatgat atacagcctt gctgaaaatt
     5701 tttgcagtag caatggctac acacttcccc gtgcagatca tttaaaccat agtcgttccc
     5761 gaggcatcgg gtcactgtac agtgaatggg gagatatggg gcattacacg actgacgctg
     5821 gttttcaatc aaatatgtat tggtcatcta gtcccgcaaa ctcaagcgaa caatacgtag
     5881 tttccctggc aacaggtgat caaagcgtat ttgaaaagct tgggtttgct tatgcgacat
     5941 gttataaaaa cctgtgattt tccgttataa tttcttaaag agtgctccat tcggagcact
     6001 cttttttaca ttccctctat agagcttgcc cttctctcac agaaaatgaa taaataaaac
     6061 tgctgcaccg caagaatcgc gttcgatgtg actatgatcg tatccggtga gtacagcttt
     6121 gatccaaagg ctggttttct gtgatgattg accagcctca gagtattgat gacaccgccg
     6181 ttcataacag atcggcatta ctatgcataa gtactgtctc atattctcgc caaaacactt
     6241 tttaatgcct ttgcgaattc atccagactt atattgtcaa ttttttgccc aacgacgact
     6301 gactccgaaa catcggccat tgcgggccat cgcgttttgt aatcagcata aattgcaaca
     6361 acgggagtat tataggcact tgccatatgt aatattgagg tgtcaggagt tattgcgata
     6421 taagcatctt ttatgattgc tgctgaacgt ttaatcgagg gtgataagga taaacggtag
     6481 acattattat aacaatccac taataccctg gctttatctt ccccttttgg tccatgaacg
     6541 atcactattg gtatatctgt ttcactttga atttttgcta ttagattttc tgcaatcgat
     6601 aatgaaaatg tacgttcctg cgagctacct tctaaattaa gcgcaatgta agatcctaac
     6661 gatcgcattt cctcgcgcac ctcatcgagt acatcctcac ttagtggcaa ttcatatatt
     6721 ggcctgactg ctggaaaacc cgcctcacgc atcataaatg cccatgtcat gggtacagga
     6781 gcccggagat tctgatccat ccgggacgcg ttcttgcaca aaggggagta gcacttcatg
     6841 gttaaaccaa caacctgaaa attcgttttt gctttcaact gactgataaa tatcatcgtt
     6901 ttcaggttct ttttacgcat cgcctctata caaagatccg gcgtaccgta ttgctgtgtt
     6961 atgttctttg ctaaatcttt tatttctttt aatgttgcgt gatcctgcat agtcattgtg
     7021 actaatgtta atttagtctt ttcaagttta agtgcattaa acacttctaa attaattgtc
     7081 gacgtaacaa ttaaaagatg cttaatttta tgcaattcaa gcgcccgaat aacaggaaag
     7141 atggccatag catcgccaat ctggtcggga atatggatga caacaaagtc tgttttttca
     7201 atattgaaat tataagcttt ataatcgtag taactaaatg caatacgtct caacaatgat
     7261 gctaaaaaca tacctaacct cgcctcccta ctggttataa tgcaatgcag tctatcagac
     7321 tcatcagggt gccattttgt gcatatgcgg acttttatgt ttcatatctc taacctgtgg
     7381 gtcctctgct taatccttaa acaacaccag caactcctgc gctttcatct tccatcgaat
     7441 ttttcatgtt gccgctaatc agccataaaa tcatttgcag atgcgctctg tcgaggtagt
     7501 ctcataaggt tcgtttatag atcgacggca atgtgagtta ccttttccat actaattata
     7561 aaaagacagt acaaacagga tcattatgga ctccacgctc atctccactc gtcccgatga
     7621 agggacgctt tcgttaagtc gcgcccgacg agctgcgtta ggcagcttcg ctggtgccgt
     7681 cgtcgactgg tatgattttt tactctatgg catcaccgcc gcactggtgt ttaatcgcga
     7741 gtttttcccg caagtaagcc cggcgatggg aacgctcgcc gcatttgcta cctttggcgt
     7801 cggattcctt ttccgtccgc tcggcggtgt cattttcggt cactttggcg accgactggg
     7861 acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc atcgcgacag ccttgattgg
     7921 tattcttcct tcattctcga ccattgggtg gtgggcacct attttactgg tgacgctgcg
     7981 tgccattcag ggatttgccg tcggcggcga atggggaggc gcggcgttgc tttccgttga
     8041 aagtgcacca aaaaataaaa aagcctttta cagtagcggt gtacaagttg gctacggtgt
     8101 aggtttactg ctttcaactg gactggtttc attgatcagt atgatgacga ctgacgaaca
     8161 gtttttaagc tggggctggc gcattccttt cctgtttagc atcgtactgg tactgggagc
     8221 attgtgggtg cgtaatggca tggaggagtc cgcggaattt gaacaacagc aacataatca
     8281 agcggccgcg aaaaaacgca ttccggttat cgaagcacta ttacggcatc ctggtgcttt
     8341 cctgaagatt attgcgctac gactgtgcga gttgctgacg atgtacatcg ttaccgcctt
     8401 cgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc tcaatattgg
     8461 tttgctggta ggtggattaa gctgcctgac aattccctgt tttgcctggc ttgccgatcg
     8521 ttttggtcgc cgcagggttt atatcacagg cgcgttaatc ggaacgttga gcgcatttcc
     8581 tttctttatg gcgcttgaag cacaatccat tttctggata gttttcttct ccataatgct
     8641 ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt
     8701 tggtgccagt tatcgctata gtggcgctgg agtcggttat caggttgcca gtgtggttgg
     8761 cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg ggaactggca
     8821 tagcgtcgcc atttatttgc tggctggatg cctgatttct gcaataaccg ctttgttgat
     8881 gaaagacaat caacgcgctt gatagtctgg cgaagatcat ccgatcttcg ccttacactt
     8941 ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca
     9001 cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg
     9061 ctgtatgagc aatctgtagt cgcattacgc aacgccattg gcaactatat tacaagtggc
     9121 gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc actgaccgta
     9181 acctgggacg gtagcacaac caatcccccc aaaacgcgcg catttggtcg ttttacccac
     9241 gcaggcagct acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa
     9301 caacttacgt tgctgtatca ggattatggt gcgcatatct ctgtgcaacc ctcgcagcat
     9361 gaaatccctt atccttatgt catcgatggc tctgaattga cacttgatcg ctcaatgagc
     9421 gctgggttaa ctcgctactt cccgacaaca gaactggcgc aaattggaga tgaaactgca
     9481 gacggcattt atcatccaac tgaattctcc ccgctatcgc attttgatgc gcgccgagtc
     9541 gatttttccc tcgcgcggtt gcgccattat accggtacgc cagttgaaca ttttcagccg
     9601 ttcgtcttgt ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc
     9661 cagatcctcg atcctgatag tccctacatt gccctttctt gtgctggcgg gaactggatc
     9721 accgccgaaa ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag
     9781 atgccagcat ggcatttaat taccgccgat gctcagggta ttactctggt gaatattggc
     9841 gtgggaccgt caaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc
     9901 tggttgatga ttggtcactg tggcggatta cgtgaaagtc aggccattgg cgattatgta
     9961 cttgcacacg cttatttacg cgatgaccac gttcttgatg cggttctgcc gcccgatatt
    10021 cctattccga gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc
    10081 ggcaggcccg gtgaggaagt caaacagcgg ctacgtactg gtactgtggt aaccacagat
    10141 gacaggaact gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc
    10201 gtagcaattg atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca
    10261 tacgggacac tactgtgtgt ttcagataaa ccattacatg gtgagattaa acttcccggc
    10321 caggctaacc gtttttatga aggcgctatt tccgaacatc tgcaaattgg cattcgggcg
    10381 atcgatttgc tgcgcgcaga aggcgaccga ctgcattcgc gtaaattacg aacctttaat
    10441 gagccgccgt tccgataata agttctgaac cttcttatca gaaatagatg tgaaggagta
    10501 agtaagacac ctggcaaata gcctgcaata atcgtgggct gtttgcttcc ttgggcggat
    10561 acgagtttta ttatcgtctt aatgatttcc acatattaaa agcaagtatg ctttcaaaac
    10621 a
//
LOCUS       DAZFVO020000163        10513 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFVO020000163 DAZFVO020000000
VERSION     DAZFVO020000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.164"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            18..1493
                     /gene="cdgI"
                     /locus_tag="ACHKNB_RS20520"
                     /old_locus_tag="pgaptmp_004104"
     CDS             18..1493
                     /gene="cdgI"
                     /locus_tag="ACHKNB_RS20520"
                     /old_locus_tag="pgaptmp_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HGU4197510.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVITEADKALREAKSEGGNKVIIHHI"
     gene            1674..3164
                     /gene="dgcJ"
                     /locus_tag="ACHKNB_RS20525"
                     /old_locus_tag="pgaptmp_004105"
     CDS             1674..3164
                     /gene="dgcJ"
                     /locus_tag="ACHKNB_RS20525"
                     /old_locus_tag="pgaptmp_004105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HGU4197511.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3207..3710
                     /gene="yeaK"
                     /locus_tag="ACHKNB_RS20530"
                     /old_locus_tag="pgaptmp_004106"
     CDS             3207..3710
                     /gene="yeaK"
                     /locus_tag="ACHKNB_RS20530"
                     /old_locus_tag="pgaptmp_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HGU4197512.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3711..3815)
                     /gene="yoaI"
                     /locus_tag="ACHKNB_RS20535"
                     /old_locus_tag="pgaptmp_004107"
     CDS             complement(3711..3815)
                     /gene="yoaI"
                     /locus_tag="ACHKNB_RS20535"
                     /old_locus_tag="pgaptmp_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HGU4197513.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3985..4431
                     /gene="yeaL"
                     /locus_tag="ACHKNB_RS20540"
                     /old_locus_tag="pgaptmp_004108"
     CDS             3985..4431
                     /gene="yeaL"
                     /locus_tag="ACHKNB_RS20540"
                     /old_locus_tag="pgaptmp_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HGU4197514.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4388..5209)
                     /gene="nimR"
                     /locus_tag="ACHKNB_RS20545"
                     /old_locus_tag="pgaptmp_004109"
     CDS             complement(4388..5209)
                     /gene="nimR"
                     /locus_tag="ACHKNB_RS20545"
                     /old_locus_tag="pgaptmp_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HGU4197515.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            5306..6487
                     /gene="nimT"
                     /locus_tag="ACHKNB_RS20550"
                     /old_locus_tag="pgaptmp_004110"
     CDS             5306..6487
                     /gene="nimT"
                     /locus_tag="ACHKNB_RS20550"
                     /old_locus_tag="pgaptmp_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HGU4197516.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6542..6889
                     /gene="yeaO"
                     /locus_tag="ACHKNB_RS20555"
                     /old_locus_tag="pgaptmp_004111"
     CDS             6542..6889
                     /gene="yeaO"
                     /locus_tag="ACHKNB_RS20555"
                     /old_locus_tag="pgaptmp_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HGU4197517.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6911..7165)
                     /gene="yoaF"
                     /locus_tag="ACHKNB_RS20560"
                     /old_locus_tag="pgaptmp_004112"
     CDS             complement(6911..7165)
                     /gene="yoaF"
                     /locus_tag="ACHKNB_RS20560"
                     /old_locus_tag="pgaptmp_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HGU4197518.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7348..8373
                     /gene="dgcP"
                     /locus_tag="ACHKNB_RS20565"
                     /old_locus_tag="pgaptmp_004113"
     CDS             7348..8373
                     /gene="dgcP"
                     /locus_tag="ACHKNB_RS20565"
                     /old_locus_tag="pgaptmp_004113"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HGU4197519.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGSDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8406..8504
                     /gene="yoaK"
                     /locus_tag="ACHKNB_RS20570"
                     /old_locus_tag="pgaptmp_004114"
     CDS             8406..8504
                     /gene="yoaK"
                     /locus_tag="ACHKNB_RS20570"
                     /old_locus_tag="pgaptmp_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HGU4197520.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8507..8581
                     /gene="yoaJ"
                     /locus_tag="ACHKNB_RS20575"
                     /old_locus_tag="pgaptmp_004115"
     CDS             8507..8581
                     /gene="yoaJ"
                     /locus_tag="ACHKNB_RS20575"
                     /old_locus_tag="pgaptmp_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HGU4197521.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8640..8888)
                     /gene="yeaQ"
                     /locus_tag="ACHKNB_RS20580"
                     /old_locus_tag="pgaptmp_004116"
     CDS             complement(8640..8888)
                     /gene="yeaQ"
                     /locus_tag="ACHKNB_RS20580"
                     /old_locus_tag="pgaptmp_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGU4197522.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9115..10323)
                     /locus_tag="ACHKNB_RS20585"
                     /old_locus_tag="pgaptmp_004117"
     CDS             complement(9115..10323)
                     /locus_tag="ACHKNB_RS20585"
                     /old_locus_tag="pgaptmp_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4197523.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            10331..>10513
                     /locus_tag="ACHKNB_RS20590"
                     /old_locus_tag="pgaptmp_004118"
     CDS             10331..>10513
                     /locus_tag="ACHKNB_RS20590"
                     /old_locus_tag="pgaptmp_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197524.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRLQIFDHDATEK
                     LRTYFSNVCADFEAELV"
BASE COUNT         2591 a         2533 c         2430 g         2959 t
ORIGIN      
        1 aacaatcatt cggaaaaatg attcagtcaa cacgtatttc catggggtta ttctttaaat
       61 attttttatc gttaacgaaa attgatcctg gtcaaaacta tatatctctg ccatcaataa
      121 aatccagcac tcacattgct ctccttttta tggtttctat gggtacacaa aaattaaaag
      181 ctcaaagctt ttttattttc agtttattgc tgacgttaat tttattttgc attactacct
      241 tatataacga aaacacaaat gtaaaactca tcccacagat gaattacctg atggttgttg
      301 tggctttgtt tttccttaac gccgtcattt ttcttttcat gttaatgaaa tatttcacta
      361 acaaacaaat tttaccaaca ctcattttaa gccttgcatt tttaagtggc cttatctatt
      421 tagttgaaac cattgtaatt atccataaac caattaacgg cagtacactg atccagacaa
      481 agtcgaatga tgtttctatt ttctatattt tccgccaact cagttttatt tgtttaacct
      541 ctctggcact cttttgttat ggaaaagaca acatccttga caacaataag aaaaaaacgg
      601 gaatcctgtt gctggcgctg attccttttt tagtttttcc ccttctggca cacaatctga
      661 gcagttataa cgctgactat tctttgtatg tcgtcgacta ctgtccggac aaccatactg
      721 cgacctgggg aatcaactat acaaaaatat tggtttgtct gtgggcattt ttactgttct
      781 ttattatcat gcgtacacga ttagccagcg aactatggcc attaatagca ttattatgtc
      841 tggcatcgct atgctgcaac ttacttctac tgactctgga tgagtataat tacaccatct
      901 ggtatatcag tcgcgggatt gaagtttcca gtaaactgtt tgttgtgtct tttctgattt
      961 ataacatttt tcaggagctg caactctcca gcaaactggc agttcatgat gtgctgacca
     1021 atatttataa tcggcgctac tttttcaaca gcgtagagtc attattgtcg cgacctgttg
     1081 ttaaggactt ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc aatgcccaat
     1141 ggggacatcg tgtgggtgat aaagtgctgg tttcaattgt cgatattatc cagcaaagca
     1201 tccgccccga tgatatttta gcgcgtctgg agggtgaggt gtttggcttg ctatttaccg
     1261 aactcaatag tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat gtcgaactcc
     1321 tgaccggctt tagtaacaga tatgatgttc ctgaacaaat gaccatcagt attggcacgg
     1381 ttttttcaac gggtgacacg cgtaatatct cgcttgtcat cacggaagca gataaagcct
     1441 tacgcgaagc gaaaagcgag gggggcaaca aagtgattat tcatcatatt taagtgcaaa
     1501 aatattcaga gccatgcttt ttgcgtggct tttgcataca atttattact attaccccta
     1561 aatttcccct ccccagcggc gcagagatga gtataattag cgcccttgtg ccaggccgca
     1621 atcgaacttt atctggtttt ctcgtttcac taaccgaagg agtgccattt atcatgaaat
     1681 tgcaccatag aatgctccgg cattttatcg ccgcaagtgt cattgtgctg acatcttcct
     1741 tccttatttt tgaacttgtc gccagcgaca gagcaatgag tgcctatctg cgctatatcg
     1801 tgcagaaagc agactcctcc tttctttatg ataagtatca gaatcagagt attgccgcgc
     1861 atgtgatgcg cgctctcgct gctgagcagt cggaagtgtc gccagaacag cggcgcgcca
     1921 tctgcgaggc ttttgagtct gccaataata cccatggctt aaacctgact gcccataaat
     1981 acccgggctt acgcggcaca ctacaaaccg catccactga ctgcgacaca attgtggaag
     2041 ctgcagcact attacccgct tttgatcagg cagtggaagg caaccgccac caggatgatt
     2101 acggttcagg tcttgggatg gccgaagaga aatttcacta ttatctcgat ctcaatgacc
     2161 gctatgtcta tttttatgag ccggttaatg ttgaatactt tgcgatgaat aactggtcct
     2221 tcctgcagtc aggaagtatt ggcatcgatc gcaaagatat tgaaaaggta tttaccgggc
     2281 gtaccgtatt gtcgagcatt taccaggatc agcgtactaa acagaacgtg atgagtttgc
     2341 tgacgccggt atatgtcgca gggcagctaa aagggattgt gctgctggat attaacaaaa
     2401 acaatttgcg gaatatcttt tacactcatg accgccctct cctctggcgt tttctcaatg
     2461 tcacgctaac cgataccgat tcggggcgcg acattatcat caaccagagc gaagataatc
     2521 tgttccagta tgtcagttac gtccatgact taccgggcgg cattcgtgtc tcgttatcca
     2581 ttgatattct ttactttatc acgtcttcgt ggaaaagcgt tctgttctgg attttgacgg
     2641 cgttaatttt gctgaatatg gtgcggatgc acttccgctt ataccaaaat gtgtcgcgag
     2701 aaaatattag tgatgcgatg actggactgt ataatcgcaa aattttaacc cctgaactgg
     2761 agcagcggtt gcagaaactg gtgcaatccg gttcttcggt gatgtttatt gcaattgaca
     2821 tggacaagtt aaagctaata aatgacaccc tcggtcatca ggaaggggat ttagcgatta
     2881 cgttgttagc tcaggcgatt aaacaatcga ttcgtaaaag tgattatgcc atccgactcg
     2941 gtggcgatga attctgcatc attcttgtcg attcgacgcc gcaaattgca gcacaactgc
     3001 ctgaacgtat cgaaaaacgt ctgcaacata tcgcgccgca gaaagagatc ggcttctctt
     3061 ccggtattta cgcgatgaaa gaaaacgata cgttacatga tgcgtataaa gcttccgatg
     3121 agcgtttata tgtcaataag cagaacaaaa acagccgttc atgataacct tctgtggttg
     3181 tttgcttgta atctcaggag cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc
     3241 agcggttaat cgctttatta tcacaagaag gtgctaactt ccgcgtcgtt acccatgaag
     3301 cggtaggtaa atgtgaagcg gtgtcagaaa ttcgtggcac cgctttaggc cagggagcta
     3361 aggcactggt atgtaaagtc aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg
     3421 ctgccgatca acaggccgat ctgagccaac tcgccagcca tataggcgga ttacgcgctt
     3481 cgttggccag cccggcggaa gtcgatgaac tcacaggctg tgtcttcggc gcgatccccc
     3541 ctttcagctt ccatccaaaa ctcaaactgg ttgccgaccc tctactcttc gaacgatttg
     3601 atgaaatcgc cttcaatgca gggatgctgg ataaatccgt tattttgaaa accacggatt
     3661 atttgcgcat tgcgcaacca gaactgctca atttccgccg cactgcgtaa ctagcctgtc
     3721 cgttcaataa gcagaacgga caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg
     3781 ataatcagtg tctcgacgaa catttgatcg ttcatagcat gcccccggga gatagctgtt
     3841 ttcacgttat tgttagcgtg cacaaatggc agtttgatga cagttcgcct gattttttat
     3901 gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa gcgtaaagta agcaggctta
     3961 ttattttttg gcaaggaaac cacgatgttt gatgtcactt tgctgatcct gctcgggtta
     4021 gctgcgctgg gctttatcag tcataacacc actgtcgccg tttcaattct ggtgttgatc
     4081 attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt
     4141 atcgggataa tcattctgac gattggcgtt atggccccca tcgccagcgg gacgctaccg
     4201 ccctcaacct tgattcactc gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc
     4261 gttattgtct cctggctggg tgggcgcggc gtgaccttaa tgggcagcca gccgcaactg
     4321 gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc tgtttcgcgg cgtaccggtc
     4381 ggcccgctta ttgccgcggg tcttgtttcg ctgattgtgg ggaaacagta gttaatcccg
     4441 caatatatcg gcctggcgtt tgccccaggc ctttcttaaa catagtaata aaggccgttg
     4501 ttgaatcata ccccagcgta tgagcaactt tctgtacagt atcgcctttc actaatccct
     4561 gaagtgccat aatcagctgt aactgctgac gccactggcg aaaactcaac cccgtctctt
     4621 ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa aaagccagcc cattgcccta
     4681 atgaccccca ctcgacgggc cctttcgcca tcatctccac catcgtgcgg attttaggat
     4741 gagaagaaac gggcaggtgc agctgttgtt gtggctgctg agggagttca tcaaacagga
     4801 cctgaattaa tctgcgggtc atcggctcag cacgctgagt cgtcgtcctg tttgccaggg
     4861 ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt gcaacatgtt gtcggcattg
     4921 tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc acttgattgc
     4981 tatgttccac cccgcccggt atccaaaccg catattgcgg cggcaccatc cacaaagcat
     5041 tttccaccgt acaggtaatt gcaccatgta atgctagtat cagctgtcct ttgcggtgct
     5101 gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg accgcggctt
     5161 catggtgtcg gtcgggttca tagccattga gattcaacct gtgcatcatt ttgtccgaat
     5221 ttagcgataa tttgtcattt tagcttgatt caacataaca ataaaaacgg taaggtacag
     5281 cctcgtttgt aacaatgaga agcatatgac ctgttcaact tcattaagcg gcaaaaacag
     5341 gattgtcctt atcgctggca ttctgatgat tgccacaaca ttacgcgtca cctttaccgg
     5401 cgcagcgccg ttactggata cgattcgttc cgcttactcg ctgacgacag cgcaaaccgg
     5461 cttattaacc accctgccat tattggcctt tgcgctaatc tcacctttgg ctgccccggt
     5521 ggcgcgacgt tttggtatgg aacgtagcct gtttgccgcg ttacttttga tctgtgcagg
     5581 tatcgcaatt cgttctctcc cttcgcctta cttattattt ggcggtacag cggtcattgg
     5641 cggtgggatt gcattaggca atgtcttact gccaggatta attaaacgcg atttccctca
     5701 ttccgtcgcc agacttaccg gcgcatattc cctgacaatg ggagctgcag cggcactggg
     5761 atcggctatg gtcgtgccgc tggctttgaa cggttttggc tggcaaggcg cgttgttcat
     5821 gctgatgtgt tttcctctgc tggctctttt tttatggctg ccacagtggc gaagccaaca
     5881 acatgcaaat ttgagtacct cgcgcgcctt acatactcgg ggtatctggc gttcgccgct
     5941 tgcctggcag gtcacattgt ttcttgggat caactcactg gtctattacg tgattattgg
     6001 ctggcttccg gcgatcctca tcagtcgcgg ctatagcgaa gcacaggcgg gttcactgca
     6061 tggtttgctg caactagcca cagcagctcc cggtttgctg atccctcttt tcttacatca
     6121 tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg atgtgcgcag tgggcgctgt
     6181 tgggctctgc tttatgcccg cgcacgcgat cacctggact ctgcttttcg gttttggttc
     6241 cggcgcaaca atgatactgg ggttgacgtt cattggtctt cgggcaagtt ctgcgcatca
     6301 ggcggcggca ctctcgggga tggcacaatc cgtcgggtat ttgctggcag cctgtgggcc
     6361 gccgctgatg ggtaaaatac acgatgctaa cggtgactgg tccgtaccac tgatgggtgt
     6421 tgccatactt tcactactga tggcgatttt cggactttgc gccgggagag acaaagaaat
     6481 tcgctaatat ccggtgctat agtgacgtga caaatcatgc gtgaaaggga gaacaaacac
     6541 gatgaatatt cagtgcaaac gcgtttatga tccggctgaa cagagcgatg gttatcgcgt
     6601 actggtcgac cgcctctggc cgcgcggtat caaaaaaacc gatttagccc ttgatgagtg
     6661 ggataaagaa atcacgccgt caacggaact gcgcaaagcc tttcacggcg aagtcgtcga
     6721 ttttgcaacc tttcgcgagc aatatcttgc agaactggcg caacacgagc aagaaggaaa
     6781 gcggctggcg gacatcgcca aaaaacagcc gctgaccctg ctctacgcag caaaaaacac
     6841 cacgcagaac catgcgctgg tgctggccga ctggctacgt agcttgtgat tttagtacag
     6901 catccggcgg ttatttttca ccagccggat ggtcccgtcg ccacaatgcc cattcatcaa
     6961 tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc ttgcgctgtc tgcactggaa
     7021 tgagcgtccc gcccttctgc tggcaataga ccgacgccgg atttgccata ccaatctgcg
     7081 gcggtttagg tgcttctggc tgagaagagg ttgaacaacc agccaggacc agcaagcaag
     7141 gcaaagcaaa actgataatt ttcatttatt gatctcacac atttatctac gcttagagta
     7201 tcgcggtatc gttttgtttt gcagcactat ttttattaca ttcactcaaa acatattacg
     7261 tcttgtttca tctttgttga tgatgtttta tcatgcctgc aaagattaaa taatcagcat
     7321 ttacccgccg tatcctggag ttgtttcgtg tcagatcaga ttatcgcccg cgtctcgcaa
     7381 tcccttgcca aagaacagtc actggaaagt ctggtccgac agcttctgga gatgctggaa
     7441 atggtcactg atatggaatc aacctacctg accaaagtgg atgtcgaagc gcgcctgcag
     7501 catattatgt ttgcccgtaa cagccagaaa atgcacatcc cggagaattt taccgtctcg
     7561 tgggattact cgttatgcaa acgcgctatt gatgaaaact gctttttcag cgatgaagtc
     7621 cctgaccgtt ggggcgactg tattgcggca cgcaatcttg gaatcaccac atttctgagt
     7681 acaccaattc acttaccgga tggatcattc tatggcacgc tttgcgccgc cagcagtgag
     7741 aagcgccagt ggagtgaacg cgcggaacag gttttacagt tattcgccgg actgattgca
     7801 caatatattc aaaaagaggc gttggttgaa cagctgcgcg aagccaatgc cgcgctgatt
     7861 gcgcaatcgt ataccgactc gttaaccggg ctaccgaatc ggcgggcgat ttttgaaaat
     7921 ctgacgacgc tgttttctct cgcccggcat cttaaccata agataatgat cgcgtttatc
     7981 gatctggata acttcaaatt aatcaatgac cgttttggtc ataatagtgg cgatctgttt
     8041 ctcattcagg ttggcgagcg ccttaatacg ctccagcaaa atggcgaagt tattggtcgt
     8101 ctcggcagtg atgagttttt ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg
     8161 ctgcgagaac gtattcaaca gcaaatacgt ggagaatatc acttaggtga tgttgatttg
     8221 tattatcccg gtgccagtct tggcatagta gaagtcgatc ctgaaacaac cgatgcagac
     8281 agtgccctgc atgctgccga tattgcgatg tatcaggaga aaaaacacaa acagaaaaca
     8341 ccttttgtcg cgcatccagc gctacattcc tgaggcgtat tcacatcctt ttgattggtg
     8401 ataacatgcg aatcggtatt atttttccgg ttgtaatctt tattacagcg gtcgtatttt
     8461 tagcatggtt ttttattggc ggctatgctg ccccgggagc ataaagatga aaaaaacaac
     8521 gattattatg atgggtgtgg cgattattgt cgttctcggc actgagctgg gatggtggta
     8581 acgtcacctc taaaaaatag caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt
     8641 taacttttaa tcttcctgta gataaatagc acgacaatcg caccaataac ggcaaccacg
     8701 aagctgccaa aattgaagcc atcgacttta ccaaagccaa acagtgtgct gatccatccg
     8761 ccgactacgg caccgactat ccccagcaga atagtcataa agaatccacc accatcttta
     8821 cctggcatga tccacttcgc cagaataccg gcaataagcc caaaaataat ccatgacaga
     8881 atgcccattg tttcctcact tatctgtttt gcattagcgg gttagtcgct gataaaaagc
     8941 atagcacaac atcgggaggg caagatttgt gacgagcatc acggaggatt tttttgcgat
     9001 ggcgcagaaa ttgcgccatc aacgatcggt gataattacc aaccacaaac atcatgttcg
     9061 ttttccgtgt cttgacatcc tccacgccct gaatgacgag gatccctgct acgttcaggc
     9121 tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact gcaccatctc
     9181 tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccgt ttacatcggc
     9241 gttcgctgta tatccacata cctggcatct gaattgactt tgtgacaggc gattttcttt
     9301 cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac
     9361 ctgaccgcca ctccagagct gcttgtactc aagctggcgg cgcatttcat accagccctg
     9421 atccagtatc gaacggttta aacctgattt tgcccggaca ttgcgccccg gctggctgac
     9481 cgtacccgct gctgactttg acatgttgct gaccttcaaa tcctcaatga ctatcattgc
     9541 gtggtttttg ctgacggtcg ttgtgacttt atgaaggtag tccctgcgga tatttgcgat
     9601 acgggaatgc agtcgctgta ttttgcgttt ctgcttctgc cagttattgc tgaatttgac
     9661 cttgcggctt aactgtcgct gaagtctcgc cagcgtcttc tggtttttct gaaaactgtt
     9721 tacaggccca aagactgtgc catctgacag cgtggcgagt ttagccacgc cagcatccag
     9781 cccgatcatt gatgctgaag ggtgaaccgg agttgatact tcactttctg tctgaatact
     9841 gatgtaccac ttaccgcagg actggctgac cgtgacattt ttcacaacac ccgtgacctg
     9901 acggctattc cggtagcgca tccagcccag tttcggcaga aaaatacggc tgttttcctg
     9961 atcgagcttt acgccctgcg ggtagcggaa tgcatcattc tgtccccgct ttttgaatcg
    10021 gggaaaagca gcccgcttcc ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga
    10081 ctgttgcaat ggctgtgagg gcgaatcttt aagccattgc gtttcagtgg cgtttttcca
    10141 ctcaaccagc caggaagcca ttttcccgta agggatgtat ttattaccgg cctcatgatt
    10201 ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg cgaagcgcct
    10261 catttcacac tcctgttgac cacctggtct taactggaat ttaaatgcct gtaatcgttt
    10321 cacaggtcaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca
    10381 ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc agataccgac tccagatttt
    10441 tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
    10501 agctgaactt gtt
//
LOCUS       DAZFVO020000164        10446 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFVO020000164 DAZFVO020000000
VERSION     DAZFVO020000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.165"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            122..697
                     /gene="ssuE"
                     /locus_tag="ACHKNB_RS20595"
                     /old_locus_tag="pgaptmp_004119"
     CDS             122..697
                     /gene="ssuE"
                     /locus_tag="ACHKNB_RS20595"
                     /old_locus_tag="pgaptmp_004119"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGU4197525.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADRLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            690..1649
                     /gene="ssuA"
                     /locus_tag="ACHKNB_RS20600"
                     /old_locus_tag="pgaptmp_004120"
     CDS             690..1649
                     /gene="ssuA"
                     /locus_tag="ACHKNB_RS20600"
                     /old_locus_tag="pgaptmp_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HGU4197526.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1646..2791
                     /gene="ssuD"
                     /locus_tag="ACHKNB_RS20605"
                     /old_locus_tag="pgaptmp_004121"
     CDS             1646..2791
                     /gene="ssuD"
                     /locus_tag="ACHKNB_RS20605"
                     /old_locus_tag="pgaptmp_004121"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HGU4197527.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2802..3593
                     /gene="ssuC"
                     /locus_tag="ACHKNB_RS20610"
                     /old_locus_tag="pgaptmp_004122"
     CDS             2802..3593
                     /gene="ssuC"
                     /locus_tag="ACHKNB_RS20610"
                     /old_locus_tag="pgaptmp_004122"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HGU4197528.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3590..4357
                     /gene="ssuB"
                     /locus_tag="ACHKNB_RS20615"
                     /old_locus_tag="pgaptmp_004123"
     CDS             3590..4357
                     /gene="ssuB"
                     /locus_tag="ACHKNB_RS20615"
                     /old_locus_tag="pgaptmp_004123"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HGU4197529.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4564..7176)
                     /gene="pepN"
                     /locus_tag="ACHKNB_RS20620"
                     /old_locus_tag="pgaptmp_004124"
     CDS             complement(4564..7176)
                     /gene="pepN"
                     /locus_tag="ACHKNB_RS20620"
                     /old_locus_tag="pgaptmp_004124"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HGU4197530.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDELWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7442..8644
                     /gene="pncB"
                     /locus_tag="ACHKNB_RS20625"
                     /old_locus_tag="pgaptmp_004125"
     CDS             7442..8644
                     /gene="pncB"
                     /locus_tag="ACHKNB_RS20625"
                     /old_locus_tag="pgaptmp_004125"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGU4197531.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8813..10213
                     /gene="asnS"
                     /locus_tag="ACHKNB_RS20630"
                     /old_locus_tag="pgaptmp_004126"
     CDS             8813..10213
                     /gene="asnS"
                     /locus_tag="ACHKNB_RS20630"
                     /old_locus_tag="pgaptmp_004126"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGU4197532.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2369 a         2701 c         2798 g         2578 t
ORIGIN      
        1 agtttatttt cagaaaaaga tacacaaaat ttatatttgg cattttcttg tctctccgac
       61 agactgaccg aattgttatc aatgttaaca aaaaaagaac aactggttat aaggagagag
      121 tatgcgtgtc atcaccctgg cgggtagtcc tcgctttcct tctcgctcca gctccttgct
      181 ggaatatgcg cgggaaaaac taaatggcct cgatgtagag gtttatcact ggaatctgca
      241 aaacttcgcc ccggaagatc tgctttatgc tcgtttcgat agtccggcac tcaagacctt
      301 caccgaacag ctgcaacagg ctgatagact gatagtcgct acacctgtgt ataaagccgc
      361 ctattccggc gcgctgaaaa ccctgctcga cctgctgcca gagcgcgctt tgcaaggcaa
      421 agtggtgcta ccgctggcga cgggcggtac cgtggcccat ctgctggcgg tcgattatgc
      481 ccttaaacca gttttaagcg cactgaaagc tcaggagatc ctgcacggcg tgtttgccga
      541 tgactcacaa gtaattgatt accatcacag accccagttc acgccaaatc tgcaaacccg
      601 tcttgatacc gcgctagaaa ctttctggca ggcattgcac cgccgcgatg ttcaggttcc
      661 tgaccttctg tctctgcgag gtaatgccca tgcgtaacat cattaaactg gcgctggtgg
      721 gattgcttag cgtctctacg attgcggttg ctgcagaatc ctcgcctgaa gcgttacgta
      781 taggctatca gaaaggcagt attggtatgg tactggcaaa aagccaccag ttactggaaa
      841 aacgctatcc gcaatcaaaa atctcctggg tggagttccc cgcgggtccg caaatgttgg
      901 aagcgttaaa cgttggcagt attgatctcg gcagtaccgg ggatattccg ccaatctttg
      961 cccaggctgc cggggctgat ttggtatatg tgggcgtcga gccaccgaag cccaaagccg
     1021 aagtgattct ggtggcagaa aacagcccga tcaaaaccgt agccgatctt aaaggtcaca
     1081 aagttgcctt tcagaaaggt tccagttcac acaacctttt actgcgtgca ctgcgtcagg
     1141 ccggacttaa gtttaccgat atccaaccca cttacctgac gcccgctgat gcccgcgccg
     1201 cgttccagca aggtaacgtt gacgcctggg ctatctggga tccctactac tctgctgcat
     1261 tattacaggg cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa actggatcgt
     1321 tttatctggc agctcgtccc tatgcagaaa aaaacggcgc ttttattcag ggcgtactcg
     1381 caacctttag tgaggccgat gcgttaaccc gcagccagcg cgagcaaagc atcgctttac
     1441 tggcaaaaac gatgggctta ccggcaccgg tgattgcctc ttacttagat catcgccctc
     1501 ctaccaccat caaaccggtt aacgccgagg ttgccgcctt acaacagcaa acggcagatc
     1561 tgttttatga aaatcgtctg gtgccgaaaa aagtcgatat tcgccagcgc atctggcagc
     1621 ccactcaact ggaaggaaaa caattatgag tctgaatatg ttctggtttt taccgaccca
     1681 tggtgacggg cattatcttg gaacggaaga aggttcacgc ccggttgatc acggctatct
     1741 gcaacaaatt gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga tcccaacggg
     1801 gcgatcgtgt gaagatgcgt ggctggtggc ggcgtcgatg atcccggtga cgcagcggct
     1861 gaagtttctt gtcgccctgc gccccagcgt aacctcacct accgttgccg ctcgccaggc
     1921 cgccacgctt gaccgtctct caaatggtcg tgcgttgttt aatctggtca caggcagcga
     1981 tccacaagag ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct acgaagcctc
     2041 ggcggaattt acccaggtct ggcggcgctt attgctgggg gaaaccgtca atttcaacgg
     2101 taaacatatt catgtgcgcg gagcaaaact gctcttcccg ccgattcaac agccttatcc
     2161 tccgctttac tttggcggat cgtcagacgt cgcccaagag ctggcggcag aacaagttga
     2221 tctctacctc acctggggcg aaccgccaga actagtaaaa gagaagatcg aacaagtgcg
     2281 ggcgaaagct gccgcgcatg gacgcaaaat tcgtttcggt attcgtctgc atgtgattgt
     2341 tcgtgaaact aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc atcttgatga
     2401 tgaaactatc gccaaagcac aggccgcatt cgcccggacg gattccgtag gacaacagcg
     2461 aatggcggcg ttacataacg gcaagcgcga caatctggag atcagcccca atttatgggc
     2521 gggcgttggc ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg gtcctacggt
     2581 cgctgcgcga atcaacgaat acgccgcgct tggcatcgac agctttgtgc tttcgggcta
     2641 tccgcatctg gaagaagcgt atcgggtcgg cgagttgctg ttcccacatc tggatgtcgc
     2701 cattccggaa attccccagc cgcagccgct gaatccgcaa ggcgaagcgg tggcgaatga
     2761 ttttatcccc cgtaaagtcg cgcaaagcta aggaagaaat aatggcaacg ccagtgaaga
     2821 agtggttatt gcgcgttgcc ccctggtttt taccggtggg catcgtggcg gtgtggcaac
     2881 tggcctcctc agttggctgg ctgtcgacgc gtattttgcc ctcaccggaa ggggtggtga
     2941 cggcgttctg gacgctctcc gccagcggcg aactgtggca gcatctggcg atcagttcct
     3001 ggcgggcgct gattggcttt tcaattggcg gatcgctggg gttgattttg ggactgatta
     3061 gcgggctatc tcgctgggga gaacggctgc tggatacctc aattcagatg ttgcgcaacg
     3121 taccgcatct ggcgctgatc ccgctggtga ttttgtggtt tggcattgat gaatccgcaa
     3181 aaatctttct ggtggcgctc ggcacgctat tccccattta tatcaacacc tggcatggca
     3241 tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag ctatggatta tccggcatac
     3301 cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc aattatggtc ggcgtgcgtt
     3361 ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga aaccatttct gccaattcag
     3421 gcattggtta tctggcgatg aatgcgcggg agtttttgca aacggacgtg gtggtagtcg
     3481 ctattattct ttacgccctg ctcggcaaac ttgctgacgt cagcgcgcag ctgttagagc
     3541 gcctctggtt gcgctggaac ccggcttatc atttgaagga ggccacggta tgaatactgc
     3601 tcgtctgaac cagggcacac cattgttgct caatgcagta agcaaacatt acgcggaaaa
     3661 tatcgtcctg aaccaactgg atttacatat tccggcaggt cagtttgtgg cggtggtggg
     3721 ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg gccgggctgg aaactccaac
     3781 cgcaggcgat gtgttggcag gcaccacacc gctggctgaa attcaggaag acacgcgaat
     3841 gatgtttcag gatgcgcgtc tgctaccgtg gaaatcggtg attgataacg ttgggttagg
     3901 ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg gctgcggtag gactggagaa
     3961 tcgcgccggg gaatggcctg ccgcactttc aggcgggcag aaacagcgag tggcgctggc
     4021 aagagcgttg attcatcgac cgggtttatt gttgcttgat gaaccgctcg gggcactgga
     4081 cgccttaacg cgactcgaga tgcaggattt gattgtgtca ctttggcagg agcacggctt
     4141 taccgtactg ttggtgacgc atgatgtgag cgaagcggta gcaatggctg accgggtgtt
     4201 gttaattgaa gagggaaaaa ttggtctgga tttgacagtg gatatccccc gtccgcgtcg
     4261 attagggtcg gtgcgattag cggagctgga agcggaagta ttgcaacggg taatgcggcg
     4321 tgggcattct gaacaactga ttcggcgtca tggttaatat gccggatgtg gcgcacaaaa
     4381 ccagcatttt caacggacta gtaggcctga taagacacgt caagcatcgc atcaggcagt
     4441 tgggcacggc tgccggatgc ggcgtgaacg ccttatccag cctacaaaac cagcaatttc
     4501 aacggagttg taggcctgat aagacgcgtc aagcgtcgca tcaggcattt tggctcaaaa
     4561 gtatcaagcc agtgctttgg tgatcttctc gtacagatcg ccagagagat tttccagccc
     4621 tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt ttggcatcat aacgtttcag
     4681 gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg ttacggctgt tgaggtcggt
     4741 aagcatttcc accaggaact ggtaaccgct gccatcttcg gcatggaacg ctgccgggtt
     4801 gctgcccgca aacgcaccaa tcaacgaacg aatacggttc gggttgctca tggtaaatga
     4861 gcgatgctgc aacagaccac gcaccgtctc cagcacattc gccgccgggc tggtggcttg
     4921 caggataaac catttatcca tcaccagacc gtcctgatgc cacttatcgt cgtactcctg
     4981 catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc gcagaaagcg ctgccagcgc
     5041 atcggtcata ttgtttgctt catggtattg cttgctcacc agcacgtcag ccagatgcgt
     5101 ttcaccaaaa gcaaggaagc gcaggcaggc attacgcaga gtgcgttttg caatatcttc
     5161 atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatccgc
     5221 cagttcagtc gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc
     5281 gatgatatcg aacagttcag ccatttcatt gacagaaggc agcgtcagga tttctgccgc
     5341 cagcgccgga tcaatcttct catcgagcag caccgcgcgg aaagcgtcag ccacatgtac
     5401 cggcagagac agcggctgcc cttgctgatg acgggcgacg ttcagcttga tgtaggttgc
     5461 cagcaaactt tgcgccgcat cccaacggga gaaatcatta cgcgcatgac gcatcaggaa
     5521 ggtcagttgc tgatcgctcc acttatattc cagtttcact ggcgcagaga attcgcacag
     5581 cagcgcaggc accggctgga agtagacatt atcaaaaaca aaggtctgtt ccgcctgggt
     5641 gacgttcagc acggaattca ccggatgacc gcctttctgc aacgggatca ctttgccttc
     5701 gttgtcatac aattcgatgg caaatggaat atgcagcggc tgtttttctg cctgatccgg
     5761 cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc tcggtttccg gattgtagtc
     5821 gtctttgacg gtcacaatcg gcgtgccgga ctggctgtac caacggcgga aatgggagag
     5881 atcgacattc gacgcatctt ccatcgcctg cacaaagtcg tcgcaggttg ccgcgctgcc
     5941 atcatggcgc tcgaaataga gttgcatccc tttctggaag ttttcttcgc ccagcagggt
     6001 gtggatcatg cgaatcactt ccgcgccctt ctcgtaaacg gtcagggtgt agaagttgtt
     6061 catctcaatg accatatccg ggcggatcgg gtgcgccatc gggctggcgt cttccgcaaa
     6121 ctgcaatccg cgcatggtgc gcacattatt gatacggttt accgcgcggg aaccaaggtc
     6181 agagctgaat tcctgatcgc ggaagacggt taaaccttct ttcaggctga gctggaacca
     6241 gtcgcggcag gtcactcggt taccggtcca gttatggaaa tattcatggc cgataacgcg
     6301 ttcaatatcg agataatctt tgtcggtggc ggtgtcggtg cgggccaaca catatttgga
     6361 gttaaagata ttcagcccct tattctccat tgcgcccata ttgaagaaat ccaccgcgac
     6421 gatcatatag atgtcgaggt catactccag gccaaagcgt tcttcatccc atttcatgga
     6481 gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg ttgccgcgat cgacgtacag
     6541 ctccagtgct acttcgcgac cagaacgcgt ggtaaaggta tcgcgcagta catcaaagtc
     6601 gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac gggtcctgcc actgtaccca
     6661 atggcgtccg ttttcgagtt cgccttgcgc aacgcggtta ccattggaaa gcaggaaggg
     6721 atatttgatt ttatcggcaa taattttggt ggtaaaacgc gccagcacgt ccgggcggtc
     6781 gagataatac gtaatatggc ggaaaccttc ggcttcacac tgggtgcaaa gcgcatcgcc
     6841 tgactgataa agcccttcca gcgccgtatt cgccgccggg cttatttcat taatgatctt
     6901 aagcgtaaaa cgctccggca aattactgat gaccagtgcg ccctcttctt ctttccaggc
     6961 ggtccacagc tcatcattaa tatgaacaga aaccagtttg aggtcttcgc cgttgagacg
     7021 aagcggagca tctgatgcac catgacggac agcctggctg accgcggtaa cgaccgtctt
     7081 ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc tggtaatccg gcgcacgata
     7141 atcgtgacgg tatttggctt gtggctgttg agtcataaat aacctttagc atcttttata
     7201 gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc tatgcagaat cttcatcttt
     7261 tcaggtacaa acgcctttat tgctacattt ttataacata caccgcgtaa tgccatcgac
     7321 cagaaaggtg gcatatggtg tgatcggggt tcaataaatt gcgaaacaag gtatactcca
     7381 gcagttcctg aagatgttta ttgtactaaa cgctcctgta cgaggacgct actgcgcacc
     7441 tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg gatacagatg cttataagtt
     7501 gcatatgcag caagccgtgt ttcatcacta ttacgatgtg catgtcgcgg cggagtttcg
     7561 ttgccgaggt gacgatctgc tgggtattta tgccgatgct attcgtgaac agattcaggc
     7621 gatgcagcac ctgcgcctgc aggatgatga atatcagtgg ctctctgctc tgcctttctt
     7681 taaggccgac tatcttaact ggttacgcga gttccgcttt aacccggaac aagtcaccgt
     7741 atccaacgat aatggcaagc tggatattcg tttaagcggc ccgtggcgtg aagtcatcct
     7801 ctgggaagtt cctttgctgg cggttatcag tgaaatggta catcgctatc gctcaccgca
     7861 ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa ttagtcgact tctcggcgtt
     7921 aaccgccggc cttgatatgt cgcgcttcca tcttatggat tttggcaccc gtcgccgttt
     7981 ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa caggaatcct ggttcgtggg
     8041 caccagcaac tacgatctgg cgcgtcggct ttccctcacg ccgatgggaa cacaggcaca
     8101 cgaatggttc caggcacatc agcaaatcag cccggatcta gccaacagcc agcgagctgc
     8161 acttgctgcc tggctggaag aatatcccga ccaacttggc attgcattaa ccgactgcat
     8221 cactatggat gctttcctgc gtgatttcgg tgtcgagttc gccagtcgct atcagggcct
     8281 gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa gccattgcac attatgaaaa
     8341 gctgggaatt gatccacaga gtaaaactct ggttttctct gacaatctgg atttacgcaa
     8401 agcggttgag ctataccgcc acttctcttc ccgcgtgcaa ttaagttttg gtattgggac
     8461 tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat attgtgatta agttggttga
     8521 gtgtaacggt aaaccggtgg cgaaactttc tgacagccct ggcaaaacta tctgccacga
     8581 taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt ccgcatatta aaaaagccag
     8641 ttaatatcat cagggagcta atcggctccc tttttttacc tttaattccg aaatctttcg
     8701 ctgcatttgc gaattctgct tgtctgattg cagatgccag gtaacatagg tatcccccca
     8761 tttacgggtg gacaagtgtt tattttttcc gactattaac agagagaata ttatgagcgt
     8821 tgtgcctgta gccgacgtac tccagggccg cgtagccgtt gacagcgaag tcaccgtacg
     8881 cggatgggta cgtacccgcc gagattcaaa agctggcatc tccttcctcg ccgtttatga
     8941 cggttcctgc tttgatcctg tacaggctgt catcaataat tctctgccca attacaatga
     9001 agacgtcctg cgtctgacca ccggctgctc ggtcattgtg acgggtaaag tcgtggcgtc
     9061 gccgggccag gggcaacaat ttgaaattca ggccagcaaa gttgaagttg ctggttgggt
     9121 tgaagatcca gacacttacc cgatggcggc aaaacgccac agcattgagt atctgcgtga
     9181 agtcgctcac ctgcgtccgc gcacaaacct gattggtgcc gtcgcgcgcg ttcgccatac
     9241 gctggcgcag gcgctgcatc gcttctttaa cgagcaggga ttcttctggg tttcaacgcc
     9301 actgattacc gcatctgata ccgaaggtgc aggcgaaatg ttccgcgttt ctacgctgga
     9361 tctggaaaac ctgccgcgta acgatcaggg caaagtggat ttcgacaaag acttctttgg
     9421 taaagagtct ttcctgaccg tatctggcca gttgaacggc gaaacctacg cttgcgcatt
     9481 atccaaaatt tataccttcg gcccgacttt ccgtgctgaa aactccaaca ccagccgtca
     9541 cctggcggaa ttctggatgc tggagccgga agtggcgttt gctaacctga acgatatcgc
     9601 gggtctggct gaagccatgc tgaaatatgt cttcaaagcg gttctcgaag aacgcgcaga
     9661 cgacatgaaa ttcttcgctg aacgcgtaga taaagatgcc gtttcacgtc tggaacgctt
     9721 cattgaggcc gatttcgcgc aggtggatta caccgacgca gtgaccattc tcgaaaactg
     9781 cggcaggaag tttgaaaacc cggtttactg gggcgtcgat ctctcttctg agcatgagcg
     9841 ttatctggcg gaagaacact ttaaagcacc ggtagtggtt aaaaactatc cgaaagatat
     9901 taaagcgttc tatatgcgcc tcaacgaaga cggtaaaacc gttgcggcta tggacgttct
     9961 ggctccgggc atcggtgaga tcattggtgg ctcccagcgt gaagaacgtc tggacgttct
    10021 ggacgagcgt atgctggaaa tgggcctgaa taaagaagat tactggtggt atcgcgatct
    10081 gcgtcgctac ggtactgttc cgcattcagg tttcggtctt ggttttgaac gcctgattgc
    10141 ttacgtaact ggcgtgcaaa acgtgcgtga tgtgattccg ttcccacgta ctccgcgcaa
    10201 cgccagcttc tgattttctt cctcaaagag ccagcgcctg ctggctcttt tttatccttt
    10261 ctgtcaagtt atctgtttgt taagtcaagc aatctatttg caaccccgcc ataacgatca
    10321 tcctgttacg gaatattaca ttgcaacatt tacgcacaaa aaataacccg cattcttatt
    10381 gcggattagt tttttcttag ctgatagcac aattttcata ctattttttg gcattctgga
    10441 tgtctg
//
LOCUS       DAZFVO020000165        10392 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFVO020000165 DAZFVO020000000
VERSION     DAZFVO020000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.166"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHKNB_RS20635"
                     /old_locus_tag="pgaptmp_004127"
     CDS             complement(228..2801)
                     /gene="clpB"
                     /locus_tag="ACHKNB_RS20635"
                     /old_locus_tag="pgaptmp_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HGU4197533.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHKNB_RS20640"
                     /old_locus_tag="pgaptmp_004128"
     CDS             complement(2931..3662)
                     /gene="yfiH"
                     /locus_tag="ACHKNB_RS20640"
                     /old_locus_tag="pgaptmp_004128"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HGU4197534.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHKNB_RS20645"
                     /old_locus_tag="pgaptmp_004129"
     CDS             complement(3659..4639)
                     /gene="rluD"
                     /locus_tag="ACHKNB_RS20645"
                     /old_locus_tag="pgaptmp_004129"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HGU4197535.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4774..5511
                     /gene="bamD"
                     /locus_tag="ACHKNB_RS20650"
                     /old_locus_tag="pgaptmp_004130"
     CDS             4774..5511
                     /gene="bamD"
                     /locus_tag="ACHKNB_RS20650"
                     /old_locus_tag="pgaptmp_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HGU4197536.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5782..6123
                     /gene="raiA"
                     /locus_tag="ACHKNB_RS20655"
                     /old_locus_tag="pgaptmp_004131"
     CDS             5782..6123
                     /gene="raiA"
                     /locus_tag="ACHKNB_RS20655"
                     /old_locus_tag="pgaptmp_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGU4197537.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6226..6351
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6227..6274
                     /gene="pheL"
                     /locus_tag="ACHKNB_RS20660"
                     /old_locus_tag="pgaptmp_004132"
     CDS             6227..6274
                     /gene="pheL"
                     /locus_tag="ACHKNB_RS20660"
                     /old_locus_tag="pgaptmp_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HGU4197538.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6373..7533
                     /gene="pheA"
                     /locus_tag="ACHKNB_RS20665"
                     /old_locus_tag="pgaptmp_004133"
     CDS             6373..7533
                     /gene="pheA"
                     /locus_tag="ACHKNB_RS20665"
                     /old_locus_tag="pgaptmp_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HGU4197539.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHKNB_RS20670"
                     /old_locus_tag="pgaptmp_004134"
     CDS             complement(7576..8697)
                     /gene="tyrA"
                     /locus_tag="ACHKNB_RS20670"
                     /old_locus_tag="pgaptmp_004134"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HGU4197540.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHKNB_RS20675"
                     /old_locus_tag="pgaptmp_004135"
     CDS             complement(8708..9778)
                     /gene="aroF"
                     /locus_tag="ACHKNB_RS20675"
                     /old_locus_tag="pgaptmp_004135"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HGU4197541.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHKNB_RS20680"
                     /old_locus_tag="pgaptmp_004136"
     CDS             9988..10353
                     /gene="yfiL"
                     /locus_tag="ACHKNB_RS20680"
                     /old_locus_tag="pgaptmp_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HGU4197542.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGTDENASTIRLN"
BASE COUNT         2666 a         2746 c         2430 g         2550 t
ORIGIN      
        1 tttttgcttt ctctgccgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagcgaaaaa
      121 tataaaaact ttatcgttcg ctcatttttc aggcaaaaca tcttaaatat agtcttttcc
      181 gtctaactta tagacaaaaa cgagccccga agggctcgtt ttatcattta ctggacggcg
      241 acaatccggt cttcattaac ttccaggcga atcactttac ccggaaccaa ttcaccagac
      301 agtatttgct gtgccagcgg gttttcgatc tgctgctgga tagcacgttt caaaggacgt
      361 gcaccataga ctggatcgta accgttctcg ctcagcagtt tcagcgcctc gtcagaaatg
      421 tggatttcat aaccacgttc ttccagacgt ttgtacagac gtttcaactg aatctgcgca
      481 atcgaggcaa tgtgctgttc acccagcgga tggaagacca ccacttcatc aatacggtta
      541 atgaattccg gacggaagtt atggcttacc acaccgagca ccagctcttt catgtgcgca
      601 taatccagtt caccgaagcg ttcctgaatc aaatcggaac cgaggttaga ggtcataatg
      661 acaaccgtat tacggaagtc gaccgttctc ccctgcccgt cagtcagacg accatcatcc
      721 agtacctgca acagaatgtt gaagacatcc ggatgcgctt tttccacttc atccagcagg
      781 atgacggaat acggacgacg acgcacagct tcggtcaggt agccaccttc ttcataaccg
      841 acatatcccg gaggcgcacc aaccagacga gacaccgagt gtttctccat aaactcggac
      901 atatcgatac ggaccatcgc ctcgtcgcta tcaaacataa agttcgccag cgccttacaa
      961 agctcagttt tccccacacc cgttgggccg aggaacagga atgaaccaat cgggcgattt
     1021 ggatccgcca accccgcacg gctacggcga atagcgttag ataccgcatc aaccgcttcg
     1081 ttctgaccaa tgacgcgatg gtgcagttct tgctccatac gcagcagttt ttcgcgctcg
     1141 ctttccatca tgcgagaaac cggaatcccc gtccaacgcg ccagcacttc agcaatttcg
     1201 gcgtcggtca ctttattacg caacagacgc atagttttgc cttcgagctg cgttgcggct
     1261 tccagttgct tttccagttc cgggattttg ccgtattgca gttcagacat ccgcgccagg
     1321 tcccccacac ggcgagcctg ttcaatagcg attttcgcct gttccagttc cgctttaatg
     1381 gtctgcgtac cagaaagcga tgccttctct gctttccact cttcttctaa ctcggagtac
     1441 tgacgttctt tgtcgctcag ttcttcgttg agcatatcca ggcgtttttt actggcttca
     1501 tcagactctt tcattaacgc ctgttgttcc agtttgagct ggatgatacg acgatcgagt
     1561 cggtcgagtt cttctggttt tgagtcaatc tgcatacgaa tgctggatgc tgcttcatcg
     1621 atcaggtcga tggctttatc cggcagctga cggtcagcaa tgtagcgatg agacaacgtc
     1681 gccgctgcaa caattgccgg gtcagtaatt tgcacatggt ggtgcaattc gtaacgttct
     1741 ttcaggccac gcagaatcgc aatggtatct tcaacagaag gctcggcaac aaacactttc
     1801 tggaaacgac gttccagcgc agcatctttt tcgatgtact ggcgatattc gtcaagcgtc
     1861 gtggcaccta cgcagtgcaa ttcaccacgc gccagcgccg gtttcagcat gtttccggcg
     1921 tccattgcgc catcggcttt acccgcgccg accatggtat gtaattcgtc gataaacagg
     1981 atgacgttgc cttcctgttt ggcaagatcg ttaagcacgc cttttaaacg ttcttcaaac
     2041 tcaccgcgat atttcgcccc agccaccagc gcgcccatat ccagcgccag tacgcggcgg
     2101 cctttcaatc cttccggcac ttcgccgttg ataatacgct gcgccagacc ttcaacgatg
     2161 gcagttttac cgacgccggg ttcaccaatc agtaccgggt tatttttagt acgacgttgc
     2221 agcacctgaa tggtacggcg aatttcttca tcacgaccaa tcaccggatc gagtttgccc
     2281 tgttcggctc gttcggtaag gtcgatggta tattttttca aagcctgacg ttggtcttca
     2341 gcaccttgat cgttcacgct ttcacctcca cgcatttgtt caatcgcttg agtaatgttg
     2401 gcggtggtcg cccctgctgc tttcaggatg tcggccagcg tgccgcgaga ctcaagtgcc
     2461 gccagaacga acagttctga cgagataaag ttatcaccac gtttttgcgc cagcttgtcg
     2521 caaagattaa gaacgcgcac cagatcctgt gatggctgga catcaccacc agtaccttca
     2581 acctgcggta aacgatttaa tgcctgattg atatctgtgc gcaactggcc agcatttatg
     2641 ccagcggatg ttaataaagg actaaccgaa cccccttcct gattcagcag ggcgctcatt
     2701 aaatgaagtg gttcgataaa ttggttgtcg tgcccgagtg caagtgattg ggcatcggca
     2761 agagcaagct ggaatttatt agtaagacga tccagacgca taactcctcc cataacggat
     2821 caaagttgct actggagatt aaatgaggtc atccctcaat tattcaaggt tattgaccag
     2881 attaatgtga aaagaaaatc acgcgtaccg gatcgtcttg attctttagg ttatatcagc
     2941 caaatgaaac ttgccatacg accggtggtc ttgtcgcgac gataagagaa gaaagtctca
     3001 ttttccgtat atgtacaacg gtcgccgccg aaaatttgct caacacccac gttcgccaga
     3061 cgctgccggg caagctgata aatatccgcc agatacttat caccatgctg aatgaaagct
     3121 gcacttgctt tagcgtctac tgccataaac gcctcgcgaa cctccacccc cacttcgaac
     3181 gcgcgtggac caattgccgg ccctaaccag gcgagaatat tttccggatt atcagcaaaa
     3241 caggaaaccg tctcttccag cacgcctgcg cacagtccac gccagccagc atgagcggcg
     3301 gcgacttccg ttcccgctcg attgcaaaac agcacaggga ggcagtcggc agtcatcact
     3361 gcgcaaaccg tgccgggcgt attgctataa gaggcatctg ctcgttttga ggcataaggt
     3421 tcgccagtaa gcttaagcac atctttgccg tgtacctgct caagccagac cggtttagaa
     3481 ggcaaattgc ccgcagcaaa aagtcgcttg cgattctcct caacgtgatc cgggttatcg
     3541 ccacaatggg caccgaggtt gagcgagtca tacgggggca agctcacgcc gccgatacga
     3601 gaagagctac aggccgcaac accttttggt agcggccact gcgggacaat cagcttactc
     3661 ataaccagtc cacttcatcc ttatgctctt cgaaatcggc gcgcatcacc tcaatcagct
     3721 ccaccatatc ttgtggaata ggcgcatgcc attccatttc gatgccggag atcgggtgat
     3781 aaagacgcag catggttgca tgaagcgcct ggcggtcaaa cttacgcagc atggagataa
     3841 atgcttccga agcacctttt ggcggacgcg gacggccacc ataaaccgga tcgcccacca
     3901 gcggatgagt gatatgggcc atatgcacgc ggatctggtg cgtacgtcca gtttccagac
     3961 gcaaccgcag acgcgtgtgc acacggaagt gttccatgat gcgatagtga gtcaccgccg
     4021 gtttgcccat cggatgcacc gccatgtggg tacgtttggt cgggtggcgg ctgatgggtt
     4081 catctactgt accgccagcg gtcatatgac caatcgccac cgcttcatac tcacgagtaa
     4141 tttcacgccg ttgcaaagat tcgaccaaac gcgtctgagc cggaacggtt tttgccacaa
     4201 ccatcaggcc agtggtgtct ttatccagac gatggacgat gcccgcacgt ggtacatcgg
     4261 caatgggcgg atagtaatga agcaacgcat tcagtaccgt gccatccggg ttacccgcgc
     4321 caggatgtac caccaggtcg cgcggtttat taatgacgat gatatcttca tcttcataga
     4381 cgatatccag cgggatatcc tgcggttcaa aacgcgcttc ttcttcaatc tcagcgttga
     4441 tggcaacctg ctcgccaccc aatacttttt ctttcggctt atcacaaact ttgccgttta
     4501 ccagcacgcg ctggtcgagg atccattctt ttattcgcga acgtgaataa tccgggaaca
     4561 tttcggccaa agcctgatct aagcgttgac cgagttggtt ttcggacacc gttgcagtga
     4621 gctgtactcg ttgtgccata tatactgctt cttcgtttat cgttggggtt ttacggcttt
     4681 gccgtttaat atagtgtgct attgtagctg gtcttaaccg ggagcaggaa cagagaatct
     4741 cccgtattac attttgagga aagtcaaaac gtcatgacgc gcatgaaata tctggtggca
     4801 gccgccacac taagcctgtt tttggcgggt tgctcggggt caaaggaaga agtacctgat
     4861 aatccgccaa atgaaattta cgcgactgca caacaaaagc tgcaggacgg taactggaga
     4921 caggcaataa cgcaactgga agcgttagat aatcgctatc cgtttggtcc gtattcgcag
     4981 caggtgcagc tggatctcat ctacgcctac tataaaaacg ccgatttgcc gttagcgcag
     5041 gctgccatcg atcgttttat tcgccttaac ccgacccatc cgaatatcga ttatgtcatg
     5101 tacatgcgtg gcctgaccaa tatggcgctg gatgacagtg cgctgcaagg gttctttggc
     5161 gttgaccgta gcgatcgcga tcctcaacat gcacgagctg cgtttagtga cttttccaaa
     5221 ctggtgcgcg gctatccaaa cagtcagtac accaccgatg ccaccaaacg tctggtattc
     5281 ctgaaagatc gtctggcgaa atatgaatac tccgtggccg agtactatac agaacgtggc
     5341 gcatgggttg ccgtcgttaa ccgcgtagaa ggaatgttgc gcgactaccc ggatacccag
     5401 gctacgcgtg atgcgctgcc gctgatggaa aatgcatacc gtcagatgca gatgaatgcg
     5461 caagctgaaa aagtagcgaa aatcatcgcc gcaaacagca gcaatacata acagaaacct
     5521 gaaatacaaa acggcagccc ttgagctgcc gtttttttat tctgttagtt gtgaaactga
     5581 agcgatttag tcactatcga tctcatcaaa tatggctcgc tttgagatat tcctcaagta
     5641 aaaaaacacc tcttcctgcg atttctcaca aaaaagattc gttgacaaaa agtgacaaaa
     5701 ttatgagatt cccatcacac attttgacat caggagcggt atgctgaatt caccaagacg
     5761 ggaagacaag aggtaaaatt tatgacaatg aacattacca gcaaacaaat ggaaattact
     5821 ccggccatcc gccaacatgt cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat
     5881 ctgattaatc cacatatcat tctgtccaaa gagccacaag ggtttgttgc tgacgccaca
     5941 atcaatacac ctaacggcgt tctggttgcc agtggtaaac atgaagatat gtacactgca
     6001 attaacgaat tgatcaacaa gctggaacgg cagctcaata aactgcagca caaaggcgaa
     6061 gcacgtcgtg ccgcaacatc ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag
     6121 tagtccttta tattgagtgt atcgccaacg cgccttcggg cgcgtttttt gttgacagcg
     6181 tgaaaacagt acgggtactg tactaaagtc acttaaggaa acaaacatga aacacacacc
     6241 gtttttcttc gcattctttt ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac
     6301 agaatgcgaa gacgaacaat aaggcctccc aaatcggggg gcctttttta ttgataacaa
     6361 aaaggcaaca ctatgacatc ggaaaacccg ttactggcgc tgcgagagaa aatcagcgcg
     6421 ctggatgaaa aattattagc attactggca gagcggcgcg aattggccgt cgaggtagga
     6481 aaagccaaac tgctctcgca tcgcccggta cgagatattg atcgtgaacg cgatttactg
     6541 gaaagattaa ttacgctcgg taaagcgcac catctggacg cccattacat tactcgcctg
     6601 ttccagctca tcattgaaga ttccgtatta actcagcagg ctttgctcca gcaacatctc
     6661 aataaaatta atccgcactc agcacgcatc gcttttctcg gccccaaagg ttcttattcc
     6721 catcttgcgg cgcgccagta tgctgcccgt cactttgagc aattcattga aagtggctgc
     6781 gccaaatttg ccgatatttt taatcaggtg gaaaccggcc aggccgacta tgccgtcgta
     6841 ccgattgaaa ataccagctc cggtgccata aacgacgttt acgatctgct gcaacatacc
     6901 agcttgtcga ttgttggcga gatgacgtta actatcgacc attgtttgtt ggtctccggc
     6961 acgactgatt tatccaccat taatacggtc tacagccatc cgcagccatt ccagcaatgc
     7021 agcaaattcc ttaatcgtta tcctcactgg aagattgaat ataccgaaag tacgtctgcg
     7081 gcaatggaaa aggttgcaca ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct
     7141 ggcggcactt tgtacggttt gcaggtactg gagcgtattg aagcgaatca gcaacaaaac
     7201 ttcacccgat ttgtggtgtt ggcacgtaaa gccattaacg tgtctgacca ggttccggcg
     7261 aaaacgacgt tgttaatggc gaccgggcaa caagccggtg cgctggttga agcgttgctg
     7321 gtgctgcgca accacaatct gattatgacc cgtctggaat cacgcccgat tcacggtaat
     7381 ccatgggaag agatgtttta tctggatatt caggccaatc ttgaatcagc ggaaatgcaa
     7441 aaagcattga aagagttagg ggaaattacc cgttcaatga aggtattggg ctgttaccca
     7501 agtgagaacg tagtgcctgt tgatccaacc tgatgaaaag gtgccggatg atgtgaatca
     7561 tccggcactg gattattact ggcggttgtc attcgcctga cgcaataaca cgcggctttc
     7621 actctgaaaa cgctgtgcgt aatcgccgaa ccagtgctcc accttgcgga aactgtcaat
     7681 aaacgcctgc ttatcgccct gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta
     7741 acgtttgatt aacgccagat tacgctctga cgacataata atgtcggcat aaagctgcgg
     7801 atcctgagca aacagtcgcc cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag
     7861 cgccagaagt tgctcaagct gaacattttc ttctgccaga tgcagcccat aagcaaaagt
     7921 agcaaagtgg cgcagagcct gaataaacgc catattctga tcgtgctcga cagcgctaat
     7981 acgatgcagc cgagcgcccc agacctgaat ttgctccaga aaccattggt atgcttccgg
     8041 cttacgtcca tcacaccaga ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa
     8101 catcgggtgt aaccccagta ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc
     8161 atttttcact gatgccagat caaccagaat acaatctttc ggtaaaggcg gtaatttgcc
     8221 gataacttgc tcagtaacgt ggattggcac actaacaatc accattccgg catcggcaac
     8281 aatatcagcc gctcgatccc agtcatgttg ctccagaatc cgcacctgat aacccgatag
     8341 tgtcagcatc ttctcgaaca ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg
     8401 acgcagtgac ggacaaagtg ttttaaatcc tttgtcgttt tcactggagt aagattcacg
     8461 catcacccga cgcaaaacat cctcaatcag atctggcggt acacccagag cttccgcctc
     8521 tgcacgacgc gaggccaaca tagatgcctc gcgctccgga acataaatag gcagtccaaa
     8581 gcggcttttc acctcgccca cttcagcaac cagttccaga cgcttcgcta ataaattcag
     8641 cagcgcttta tcgacttcat caatttgatc gcgtaatgcg gtcaattcag caaccataat
     8701 aaacctctta agccacgcga gccgtcagct gcccgttcag atcctgatga atttcacgca
     8761 gcaaggcatc ggtcatttcc cagctaatgc aggcatcggt tacggataca ccgtatttca
     8821 tttcactgcg cggttgctca gaagactgat tgccctcgtg gatattactt tcgatcatca
     8881 gaccaataat tgagcgattg ccatctttga tttgagcaac cacggattct gccaccgcag
     8941 gctgacggcg ataatcttta ttggaattac cgtggctgca atctaccatc agagacgggc
     9001 gcagtcccgc ctgttccatc tctttttcac attgcgcaac atccgcaggg ctatagttcg
     9061 gcgctttacc accgcgcagg atgacatgcc cgtccggatt cccctgagtt tgtagcaacg
     9121 caacctgccc tgcctggtta atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag
     9181 cgttaattgc tgttgccaga ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa
     9241 gcccggaggc catttcacgg tgagtttgcg attccgttgt acgagcacca attgctgacc
     9301 agctaaacag atcgcccagg tattgcgggc tattcggatc taacgcttcc gtcgccagtg
     9361 gcagtcccat attcaccagc tcaagcagca atttacgcgc gatctgcagc ccggcttcta
     9421 catcaaaaga gccatccata tggggatcgt taattaaccc tttccagccg acagtggtac
     9481 ggggtttttc aaaatagacg cgcattacca gatagaggct atcgctgacc tctgcggcaa
     9541 gggctttaaa tcgacgagca tattccagag cagtttccgg atcatgaatg gaacaaggac
     9601 cacatactac cagcagacga ggatcgcgcc cggcgataat atctgaaatg gttctacgcg
     9661 agtcagcaat ctgggcttct tgttgcaggc tcaatggaaa agcggccttc agttgttccg
     9721 gagtcattaa aacctgttcg tcggtaatat gtacgttatt cagcgcgtct ttttgcatga
     9781 tggcgatcct gtttatgctc gtttgcgata gttgatcctc agcgaggatg acgtaacgat
     9841 aacacataaa gtaaagtttt caatccatat ttcgtacatt tttctttaca taagcaattt
     9901 attagcgttt tcaacccttg cttctgtata gataaattta cacccccttt gaaaacaatt
     9961 cgctatgctt tgaaaaagga gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct
    10021 ttactggtta gcggatgcca gattgatcct tatactcacg cgccaacctt gaccagcacc
    10081 gactggtatg atgttggtat ggaagatgcg atatcgggca gcgccataaa agatgacgat
    10141 gcatttagcg attcacaggc ggatcgcggt ctatacctta aaggatatgc cgaaggacaa
    10201 aagaaaactt gccagaccga ttttacttat gcccggggac tttccggtaa aagctttcct
    10261 gccagctgta acaatgttga aaatgccagc caactgcatg aagtttggca aaaagggact
    10321 gatgaaaatg ccagcacaat acgattaaat taattaaaac cccacaaata aattaaacat
    10381 aagattttac tc
//
LOCUS       DAZFVO020000166        10292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFVO020000166 DAZFVO020000000
VERSION     DAZFVO020000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.167"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1185..1379)
                     /locus_tag="ACHKNB_RS20685"
                     /old_locus_tag="pgaptmp_004137"
     CDS             complement(1185..1379)
                     /locus_tag="ACHKNB_RS20685"
                     /old_locus_tag="pgaptmp_004137"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4197543.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(1637..2104)
                     /locus_tag="ACHKNB_RS20690"
                     /old_locus_tag="pgaptmp_004138"
     CDS             complement(1637..2104)
                     /locus_tag="ACHKNB_RS20690"
                     /old_locus_tag="pgaptmp_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197544.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRIYQARIKAS"
     gene            2264..2518
                     /locus_tag="ACHKNB_RS20695"
                     /old_locus_tag="pgaptmp_004139"
     CDS             2264..2518
                     /locus_tag="ACHKNB_RS20695"
                     /old_locus_tag="pgaptmp_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HGU4197545.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            2518..2934
                     /locus_tag="ACHKNB_RS20700"
                     /old_locus_tag="pgaptmp_004140"
     CDS             2518..2934
                     /locus_tag="ACHKNB_RS20700"
                     /old_locus_tag="pgaptmp_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HGU4197546.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            3832..4194
                     /locus_tag="ACHKNB_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /pseudo
     CDS             3832..4194
                     /locus_tag="ACHKNB_RS20705"
                     /old_locus_tag="pgaptmp_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4198..4553
                     /locus_tag="ACHKNB_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /pseudo
     CDS             4198..4553
                     /locus_tag="ACHKNB_RS20710"
                     /old_locus_tag="pgaptmp_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            4699..5838
                     /locus_tag="ACHKNB_RS20715"
                     /old_locus_tag="pgaptmp_004143"
     CDS             4699..5838
                     /locus_tag="ACHKNB_RS20715"
                     /old_locus_tag="pgaptmp_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HGU4197547.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            5958..7226
                     /locus_tag="ACHKNB_RS20720"
                     /old_locus_tag="pgaptmp_004144"
     CDS             5958..7226
                     /locus_tag="ACHKNB_RS20720"
                     /old_locus_tag="pgaptmp_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGU4197548.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            7234..8379
                     /locus_tag="ACHKNB_RS20725"
                     /old_locus_tag="pgaptmp_004145"
     CDS             7234..8379
                     /locus_tag="ACHKNB_RS20725"
                     /old_locus_tag="pgaptmp_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGU4197549.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            8847..8993
                     /locus_tag="ACHKNB_RS20730"
                     /old_locus_tag="pgaptmp_004146"
     CDS             8847..8993
                     /locus_tag="ACHKNB_RS20730"
                     /old_locus_tag="pgaptmp_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197550.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(9064..10287)
                     /locus_tag="ACHKNB_RS20735"
                     /old_locus_tag="pgaptmp_004147"
     CDS             complement(9064..10287)
                     /locus_tag="ACHKNB_RS20735"
                     /old_locus_tag="pgaptmp_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGU4197551.1"
                     /translation="MNWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREM
                     IEFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGA
                     HTLTFNAVTPCHLAVLPLLASGTLPNYKFNKKPYFILEKTAHATLQINRGLLQQFGET
                     IRVDFQHKNDIEEAFKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYID
                     DAHGMSIYGENGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKT
                     YGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKG
                     TTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAIHTRENI
                     DSLHKNFIECINEQG"
BASE COUNT         2597 a         2499 c         2443 g         2753 t
ORIGIN      
        1 aactactctt tgcaccagaa acgcgcagga gtgttttagc gtgtctccgg cgtgatgtcg
       61 agttgtgcgg taatgagtga gcgaaaaaaa tcccctggat tacaggggga acccatgatc
      121 agactttgaa gtttatttgt tatttgatgg aatgagcacg aatcccggag atgacgttca
      181 ggtggcagga aacgcgctca gaagctttct aacgcacttt tttgcatctg gcaatgggtt
      241 tcatatgttt tggtgggttc aggctgtcag agacgttttg agcgcgtttc tggcgcttgc
      301 tgctactgcc aaaccatccc catggccagt tgatgccaga aacgcgcagg aattttgtag
      361 cgcgtctccg gtccctgatc aatgaattcc ctgtcattaa catgcgttta cccggaaacg
      421 cgccctgatg ttctatcgta tcgcgggaac ggtgtcgagg gtgtcggggc aacgccctga
      481 ccaggtggta atcgtatagc cgtgcgtgcg cggttatacg attacacatc ctgtcccgga
      541 atcaggattg tttttcgcgc acaactgcgg caaattcatc ccgttgcggt tgcaacgatg
      601 ggtatatcaa aactacgaaa attattcagg catttgctac cccccttgcc ctggcctcca
      661 ccagtcacgg gggatttttt cgcctgtcct gattgtcttt tgccgatggt tacggcagat
      721 tggaaaagag cgcatttgtg gtgctctctc caaaataata tttctcatta ctcaaactct
      781 gatggcttcc ccctgtaatc cagggggatt ttttaattta tcgctatgct tcgtaattca
      841 ttcagaatac agacagtatg gttaatcagt ttttcatcat tttttcggtg tttgccctgt
      901 cctgcatcag aacgctaaaa aaacaggatt ggaatctctt tatgacgaat cctccatttc
      961 aggatttttt atgtaacgtc tactacatgc gatccgttgg tgaaccgtta gttatatatt
     1021 ggttatatgt tagttatatc ttagttatac ttttgctgcg cgtcagttac agaggcgttt
     1081 tgagggtaaa ttctggaggc acgtgaccga ctgtatacaa aatgctggcg cattttgata
     1141 cgtcgacgtg ccaaagggga cgcccctttg aaaccccgcc agaattaaaa gcggcctgtc
     1201 tttttacagt attcagctct ggctatacac caacctatat tttctccttt ggcataatga
     1261 agcgcatcat tccatttttt ccatgctcca atgtaatcac cttcacgctc cagtctgaca
     1321 gcttcgtttg cgggtgaaaa atatttattt tctaaataaa ttgctttgtc ctgattcata
     1381 ttcgtaattt atatttttat ttgaattaaa aaagagcgag cccatcgggt taatacatat
     1441 tggcatgact gttgatatat cagtccaaca gcacgctcgt aatcgtttta acgtgatttt
     1501 cgcaacatgg caatccgggt tcatctgtcc ggtaatcaat aaaaaccgtg cgaaaaacgg
     1561 gacaggatgt ggccattaat gctaatgtat gcatacatta gcattaatgg ctctgacaaa
     1621 taccaggcat ccagtctcag gatgccttga ttcttgcctg gtagatgcgc tttacccgtt
     1681 caatttctgc cagcgttgcg gaccagtgta gttcggcggc atgacgatat ctggcagcca
     1741 ttttttccac catctccgga ttgctgtccg cgctgcggtg ctgacgcccc ttctccccaa
     1801 aaacttcaac aaccagccac tggcttcctg ttctgccgaa caatgccttg cacaaacgtc
     1861 tcacagtcag cggggttaaa tctggatcgg gatcgtaggc agtggtagca aaaataaagc
     1921 gcagcatttc tgatgtcttg taacgcttta cggcagctgc cagacgtgcg gcgcgttggg
     1981 gtgcttccca gcggtcgtca ctgtaattag caacttcagc cagataatcc ttttcaaggt
     2041 tttcagccgt gtaacacctg ccgcctaaaa aaaaccgcat ggcctccctg gttttgtctc
     2101 tcacatatca tccctcatta atgctaatgt atgcatacat tagcattaat atctgacagc
     2161 aacaagcggc gaagactacc cctaatcaag tacatttctt gcattgtact atttcatgcc
     2221 ctacaatgta gtaacgtacc tatatacatc aaaaggcaac agcatgaaga ttacaaccct
     2281 atccagtcgg gaacttaatc aagatgtaac tcgagccaaa cgcgccacga attatggtcc
     2341 agtatttatt actgatcggg gtaaaaccgc acatgtactt cttagtatcg aagaataccg
     2401 aaaattgacg caacaacgac gaactctcat tgacattctg gcgatgcccg atgctgatgc
     2461 agttgaattt gaaccgcctc gtattacggt tagtttaaaa cctgcggagt ttttatagtg
     2521 tatattcttg atactaatgt tatctctgag ctacggaagg ctcggacagg acgtatagat
     2581 aataatgttg caaaatgggc attgcataca gatgcggcag atttctttat ttctgttatt
     2641 tccataatgg aacttgaaca gggaatatta caagttgaac gtcgagatat gcgacaaggg
     2701 attatgctac gttcttggat ggatcagcat gtgttaccag cgttttctga taagatactt
     2761 cctgtagata ctgcggttgc attacgttgt tctcgtttac atgttcccga caggcgcagt
     2821 gaacgagacg cattgatcgc agcaacggcg ctggtacatg ggatgacggt tgtaaccaga
     2881 aatgttggtg attttgaatt aaccggagta tcaattatta atccctgggc ttgatatcgc
     2941 ttcccttctt ctaggggtcc gcttggaaaa cggaaatttt cccacgctaa gagaattgag
     3001 ttgaacgagt ggttacatcc aagccagcta actcactgaa cgtaaaggct gtacgaaatg
     3061 ttccgaccgt ccggattcct gacagtcatt tggctaaaaa atagtcttaa cgtggtttat
     3121 tttccgaatt ccaggctgac cccataaaag catccttcag ttcagcgtcg attcttaaac
     3181 tcatattaac ctgtgccatt catcacctcg tcattaccac gtatatacac agtatataat
     3241 tattcagata tcgtcaccat gatatgccgc gccagcaaca tggaaggcgg cactccatgc
     3301 tgtatatggc agaggagtaa aaccgccgaa tcccggcgta agccggtaca gtcgtccgaa
     3361 tgtcagtagt tctactcccc cctgcgaggc cagtgctgac gcgggacatg gaacgggtgc
     3421 tggagcgaag tggggaagct ccggtatact ggccaaacct acacgcctcc ttccgtctcc
     3481 gggaagatgc tggcttcgta ccctgcgtgc gtctgatctg tagctggccg tggcacggct
     3541 acggacagat tctgctccgc aacagtatcg gcgtgcctga cctcctgaaa gtcccgtaac
     3601 gcattttctc acctcccatg ccctgacgct attgagcgcg ttacagaaca atttaagtca
     3661 tttaacgagc ctccaacggc tcgatcgaaa taaatcaaat cgtaatcgtc aacgatatat
     3721 gagaggagac cccgctcgcc gcaaatcgat gaccgaacgt agtgagcgaa tcgacgagga
     3781 agcggaagag aacctgtacg aaaatggagc aattacgccc caccgtcaag aaatgagctg
     3841 gctctccacg gcgcgccatc atagacagat gtggctctgc aactgcgcca tgcgatcgga
     3901 gaccgaccag taattatctt taatgcccgg ttcgacaccc gcattctgaa acagactgct
     3961 gccgcacata gcgatccggc tgactggctg gaagaactga cagtatattg tgcgatggaa
     4021 ctggcagcag gatattatgg ggccaccaac cgctatggca cgatttctct ggcctgtgct
     4081 gccagcaggc cggactgaca tgggaagggc tggcacactc agctgatcgc cgacacacgg
     4141 atgacgtcag gggtggtaaa cgctattact gcatatcatc aggaattgct gcagcttatg
     4201 tactgcgata accctgaact caactatgca aacctgaaat ccctgcaaaa acagctggat
     4261 gaaatgtata cctgcatccg taataatcag atgatgctcc caaactacgg aaaaatgcgc
     4321 cggtatggtg aaccggtttc aacaacgttt gtagagtcga caatcaacga agtgatcgcc
     4381 agacgatgac ccaaaagcaa cagatgcagt ggggtaggga aggtacgcat tatttgttac
     4441 agaccagaac cgctgtcctg aataattaac tgcaggacaa attcgccagt tggtacccgg
     4501 gttgcacgat agacgctcag agtggaggaa agttatccgc catggcagca taactacttc
     4561 cccagttttt tcagctctcc cttaccaata tcctacgacg atcacatcga aaaaaaaaca
     4621 cggaacattt caccactgga tacgctaata gatcggatat gagcacatat tgcgcccgcc
     4681 tttcatgcag agcataaaat gaacaagatt cacgctaatt attacctgca agattctacc
     4741 gcacgtcaga tagttcaacg cacaatgggg attattccgt actcggtaaa tgtgatgaac
     4801 gagcatggga tgatcatcgc ttccggggaa gcctcacgtt tacatcaacg ccatgaaggt
     4861 gcggtactcg cattgaccga aaaccgggtc gtcgaaattg attccgcgac cgccaataag
     4921 cttaaaggcg ttaagcccgg aatcaattta ccgattaatt ttcgcgacca gctgattggt
     4981 gtactgggca ttaccggcga gcctggtgaa gtgaaggcat atgcacagtt ggtgaagatg
     5041 gcttcggaat taatcattga acaaatggct ctcctcgagc aaaaacagtg ggataagcgt
     5101 taccgcgaag agctgattaa tcagctgatt ttacgcgatc cgccaccgga ctcactgaat
     5161 gcggtggtct catggttggg aattaatctc cagcttccgc gggtggtggt gattatggaa
     5221 ctgcaacaac ctgatcgcga cgcactgcat aatctgatgg agtattttga atatcgcgcc
     5281 cgcgaccatt tggtcacgtt ttccgacttt aatgatctga taatcttaaa acctatctcg
     5341 ctaaaaaacg gcgtctggga taaatcgttg gaattaagcg atctgcagag acttaaacat
     5401 tacgcccagt cctgtggttt ttcccgactg attgtcggcg gctatttctc tggagaaaac
     5461 ggcctgcatc gctcttttca gaccgcgaaa tccacccagt ccatggcgaa aagactgaag
     5521 ttgaagcatc agttcatttt tcatgacgac tatgcgctgg catcattatt agatgggctt
     5581 tctggtacct ggcaagcgga agaattatcc aaaatctggc gcaatctggt tgccaaggac
     5641 accaaaggta tgctgcaaaa aacgctgcag aattattttg atcataattg tgatctttcg
     5701 caaacagcga ctggcttgca tattcatgtc aatacattac gctatcgact gcagagagtg
     5761 gaagatatta caggaataca aatcaataac ttaaagcagt tattctggct gtatatcggc
     5821 atggagttac aacgctgaac tgtatttccc tccaaatttt tcgccgtctg acgtcgtatt
     5881 tttagccgat acacacaagt ctaacgcggc tgctttctcc atactgcccc ctgttatttt
     5941 catggagaaa gcagaagatg gtttcaataa cagcaactgg cgccatcatc gcactcgtgg
     6001 tggcgatcgt tttaatttta cgcaaagtct cgccggctta cggcatgatt gtcggtgcgc
     6061 tggtaggcgg tctggtcggc ggcgccgatc tggtgcaaac cgtgacgcta atggtcactg
     6121 gcgcgcaagg cattaccaat gccgtgctgc gcattctcgc agcaggtgta ctggccggcg
     6181 tgttgataga atccggcgcg gcaaacacca tcgctgaaac tgtggtgaaa aaagtggggg
     6241 aaagccgggc gcttttagcc ttagctattg cgacgatgat tctgaccgcc gtcggagtat
     6301 ttatcgacgt ggcggttatc actgttgcgc cgattgccct gtctattgcc cgtcgtgccg
     6361 gtttgtcgaa actcgccatt ctgctggcga tgattggcgg tggtaaagcg ggtaatgtta
     6421 tgtcgcctaa ccccaatgct atcgccgttt cggacacctt tcatgttccc ctcacctccg
     6481 tgatgatgac cgggataatc cccggtctgt tcggcctgat cgtggcttat ttcctggcaa
     6541 aatgtctcag ccagaaaggc ctgatggtga tgcccgacga aatgatttca cacgacaacc
     6601 acggcaagca gccgggattt cttgccgcta tcagcgcgcc gctcgtcgcc attgtgctgc
     6661 tggcgctacg acctgttgcg ggtatcgtca tcgatccgct gattgccctg cccgtcggtg
     6721 ggttagccgg tgcgatcctt atgggcaggt ttaagcaaag taacgccttt atgatgtccg
     6781 ggcttacgcg catggcgccg gtagccatca tgctgctcgg taccggtacg ctcgccggga
     6841 ttatcgccaa ctcggaactg aaagatgtgc tcattcatgg actgacctct tccggtctgc
     6901 cttcttatct tctggcccct gtttccggcg cgatcatgtc catggcaaca gcctccacca
     6961 cggcgggaac cgctgttgcc gccggggtat tcagccagac gctgctgggg cttggcgtat
     7021 cagcccttgc tggttccgcc atgattcatg ctggcgcaac cgtacttgat cacctgcctc
     7081 acggcagctt cttccacgcc acgggcggca gtgtcggcat gctgatccat gaacggttaa
     7141 aactgctgcc ttatgaaacc cttgtcggtt ttgtggtcgc cctgatttcc acactgatgt
     7201 ttggcgtctt cggacttgcc gggtaaagac acgatgaaaa tagtcattgc accagattca
     7261 tttaaagaaa gtttatcggc catgcaggtg gccaccgcta ttgagcaggg atttcgtgaa
     7321 atttatcccg aggccgagta tgtcaaattg ccgatggctg acggcggtga gggtaccgtc
     7381 gaatcaatgg ttgaagcaac cgaaggccat tatctgttcc agaatgtcac cggaccgctg
     7441 gggacgccag tacaggcgcg ctggggtatg ctgggagatg ggaaaacggc gatcgttgaa
     7501 atggcctccg catccggact acatcatgtt cctccagaat gccgtaaccc tatgcttacc
     7561 accagttatg gcacaggcga actgatcctt gccgcactgg accacggcgt tgagcgaatt
     7621 attctcggga ttggcggtag cgccaccaat gacggcggcg caggtatgct acaggcactt
     7681 ggtgtggcga tgcttgatac aaacgggaaa tcgcttcctt tcggcggcgg cgcgctaagc
     7741 aacctggcgc tcatcgatgc ctcaggctgc gacgctcgac tgaaaaacgt ttctgtcacc
     7801 gtcgcctgcg acgttaacaa tccactctgt ggcgcaacag gagcgtcagc catcttcggc
     7861 ccgcaaaaag gagcaacacc agaaatggtt gcctccttag aagctgcgct gaccaacttc
     7921 ggtatacgac ttcaggacgc aaccggacga aacgtgatga cgacgcctgg cgcaggtgcc
     7981 gctggcggca tgggcgcggc gctgttaggg attctgaatg cggagttaaa gccgggagtg
     8041 gagattgtta ttgacgcgct gcatctcgat agcctggtcg cggatgccag cctggtaatc
     8101 accggtgaag gtcgtcttga tagccagagc atcagtggta aaacaccgat tggtgtcgca
     8161 cgctgcgcta agcggtatca gaaaccggtg atcgcactgg cgggcggctt aacgaccgac
     8221 caccagatag tgcatgagca cgggctggac gcggtgttct ccgtgctgac gcaaatcgcc
     8281 actctgccgg aagcgctaca ggatgcggaa tacaacctcc gcgtttcggc acgcaatatt
     8341 gccagcgtct ggaaattatc acagcagcag atgcgctaat cttcagtcag gccggcaagc
     8401 agtagctggc ctggatattc tttctcacca ggcccatgct aaaacagcct gcttcagtca
     8461 tcccacgcaa ggcactagca tggcaaaaaa agaggggagt agtggatccc aaaagacggt
     8521 taagcggtta aattgaatgg tcaataaggg ggctgtttgg gaaacgaatc tatggtcact
     8581 cccgtttttg caacaccgat tttgacgata agtgggcttg cttgaatcta tccggcgtct
     8641 gaatgggatt ttattcccgc gcctcgatga gttccgcgcc tgatgaacct caagaaaata
     8701 tacggcttca atgagccttt ccgtttcaca ggttcctcaa caggccggtg ggccgttcat
     8761 atcatcaata ccgtgatgct gatactccgt acccaccgga accgcacctg atgcggttcc
     8821 ggcttaaggg ctgtcaggac tcgcttttgg ctgccatcaa caaaccggaa gagatgccgg
     8881 attatctctt ccgtcaactc ggttttaatc agacctggca tgagtggaag cgggacgaac
     8941 agcacaggca acaacaacgc cggcccggac actaccgggg catgagtatg taatgtcagg
     9001 gacagctata cgccataaaa aaatgaaata gatggcctct ctataaagag gccatgatat
     9061 ttactaaccc tgttcgttaa tacattctat aaagttttta tgtaggctat ctatattttc
     9121 tcgggtatga atagctgata ttgcacaacg tagaattgcc tttccctctt ccactgtagg
     9181 atacatagca acggttgtag caaaaccacg atcatgcatt tttttgcata tcgttatagc
     9241 tttttcttca ttgccaatta acaatccacg tattggcatt gtagtccctt tgtttatgac
     9301 ttcggtttca ttaaatttgt catcaaagta ttttataact tgttgtaagt cattttgacg
     9361 caaggatact gtgccatcga ggtgtaaatc tgctgctgcg atacatgcag caattcctgg
     9421 taatgatggt ggaccaccaa aagcataggt agttccatac gtttttataa agtttatggt
     9481 gtcaggataa ttgcatgcaa taaatccacc atgagagcca aacgctttag ataatgaacc
     9541 agcgataata attctgtcat caagtttgtt atccagacaa gacattacat atccggagcc
     9601 attttcccca tatattgaca tgccatgagc atcatcaatg taagcatacc catcaaatct
     9661 gttagccatt ctaacaataa attcgacagg agccacccca cccatcgaac cgacactatc
     9721 agatatagat attggtacca tattatttat tgaagcatat ttaaacgctt cttctatatc
     9781 atttttatgt tgaaagtcaa ccctgattgt ttcaccgaac tgttgtaata gtcctctatt
     9841 tatttgcaat gttgcatgcg ctgttttctc taaaataaaa tatggttttt tattaaactt
     9901 gtaattaggt agtgttcctg atgcaagaag tgggagtaca gctaaatggc atggagtaac
     9961 cgcattaaat gttaatgtgt gcgccccatt cattatttta tttaatctct tttccaattg
    10021 ctcgaataaa tctactttta cgcgcgttct ggcgaccgaa agttggactc caaatctttc
    10081 aattgcatta gcgattgcat gttttagatt ttcatgtgtt tccagtccaa gataagagca
    10141 tgacacaaac tcaatcatct ccctcccatc attaacagtg attaccttac catttcttct
    10201 ggtgatcact agttcattta tatgttcgtt aaatactctt gtttggtatt tgattgaacg
    10261 ttctatcttt ttcttttgcc aattcatttt tt
//
LOCUS       DAZFVO020000167        10215 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFVO020000167 DAZFVO020000000
VERSION     DAZFVO020000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.168"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..614)
                     /locus_tag="ACHKNB_RS20740"
                     /old_locus_tag="pgaptmp_004148"
     CDS             complement(<1..614)
                     /locus_tag="ACHKNB_RS20740"
                     /old_locus_tag="pgaptmp_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HGU4197552.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEI"
     gene            881..2380
                     /gene="ansP"
                     /locus_tag="ACHKNB_RS20745"
                     /old_locus_tag="pgaptmp_004149"
     CDS             881..2380
                     /gene="ansP"
                     /locus_tag="ACHKNB_RS20745"
                     /old_locus_tag="pgaptmp_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HGU4197553.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /locus_tag="ACHKNB_RS20750"
                     /old_locus_tag="pgaptmp_004150"
     CDS             complement(2495..3556)
                     /locus_tag="ACHKNB_RS20750"
                     /old_locus_tag="pgaptmp_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HGU4197554.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHKNB_RS20755"
                     /old_locus_tag="pgaptmp_004151"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHKNB_RS20755"
                     /old_locus_tag="pgaptmp_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HGU4197555.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHKNB_RS20760"
                     /old_locus_tag="pgaptmp_004152"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHKNB_RS20760"
                     /old_locus_tag="pgaptmp_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HGU4197556.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHKNB_RS20765"
                     /old_locus_tag="pgaptmp_004153"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHKNB_RS20765"
                     /old_locus_tag="pgaptmp_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HGU4197557.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHKNB_RS20770"
                     /old_locus_tag="pgaptmp_004154"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHKNB_RS20770"
                     /old_locus_tag="pgaptmp_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HGU4197558.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /locus_tag="ACHKNB_RS20775"
                     /old_locus_tag="pgaptmp_004155"
     CDS             8532..8981
                     /locus_tag="ACHKNB_RS20775"
                     /old_locus_tag="pgaptmp_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGU4197559.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHKNB_RS20780"
                     /old_locus_tag="pgaptmp_004156"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHKNB_RS20780"
                     /old_locus_tag="pgaptmp_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HGU4197560.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHKNB_RS20785"
                     /old_locus_tag="pgaptmp_004157"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHKNB_RS20785"
                     /old_locus_tag="pgaptmp_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HGU4197561.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHKNB_RS20790"
                     /old_locus_tag="pgaptmp_004158"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHKNB_RS20790"
                     /old_locus_tag="pgaptmp_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HGU4197562.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2478 a         2484 c         2479 g         2774 t
ORIGIN      
        1 ataatttcat tcctttttaa tacttcttgt aatttcggta actgacatac agcatcagca
       61 atcgcactga tattcgtggc attatcctga aaccattcat gcccaggccc ccatgtgcgc
      121 atagtgcaaa gataacagtc caccagcgtt agttgctcac caaacgcata aggttcagcg
      181 gtcaattgtg aattcagcca gatataaagc gatttacgat attcaattac attcttcttt
      241 aactgttcag gtgcgtcagg agcccaacgc tcagggtaat cggcgaaagt gaatgttgga
      301 tagacattag caaccagcca gaccaatagc cgttgaaact gctgacgttc ggcgcgccca
      361 accggtggcg caaggtccgg gcgacgatcg agaaccatca atgcaatcgc cgctgtctcc
      421 gtcataatct cgtcattttc cagcgccagt gtcggaatct gacacaacgg gttcagggtt
      481 ttcaataact cgcgtgaagt tccctcgtgg tcaaaaccac tgacatcaac gaactgataa
      541 ggaatatcag ccagtgttag catcagctca ctgattgtag agccccatcc gggtacgcca
      601 tacaccttaa tcatatgccc ctcacaatct tttaaagatt aagtgtagga cagtacacag
      661 gttaaccagc aaaaggattt gtcatttttt cgtcagttgt attaatcccc ttcaggattg
      721 taaggggatg ttttgcatcg tttatcgata acgggtagga tgctgcgccg atagttttat
      781 acccataaag aataatggtg ataactatca tcgccaggat gaataaacat tgttcatggc
      841 aacttatatg actttttcat taaagcaatc agggagagca atgagtaaac acgacaccga
      901 cacttcagat caacacgccg cgaaacgccg ctggcttaat gcccacgaag aggggtatca
      961 caaagcgatg ggcaatcgcc aggtacagat gatcgccatt ggcggcgcga ttggcaccgg
     1021 cttgttttta ggtgcaggag cccgactgca aatggcgggt ccagcactgg cactggttta
     1081 tttaatttgt ggcttgtttt cgttttttat tctgcgtgca ttgggtgagc tggtgctaca
     1141 ccgcccttcc agtggcagtt ttgtctctta tgcccgtgag tttttgggtg agaaagccgc
     1201 ttatgttgct ggctggatgt acttcatcaa ctgggcgatg acggggattg ttgatattac
     1261 cgccgtcgct ctgtatatgc attactgggg tgcgtttggc ggcgtgccgc agtgggtctt
     1321 tgcgctcgct gcacttacca tcgttggcac catgaatatg atcggtgtga aatggtttgc
     1381 ggagatggag ttctggtttg cgcttattaa agtgctcgcc attgtgatct ttttggtcgt
     1441 gggtacagtg ttcctcggta gtggtcagcc gctggatggc aacaccactg gctttcattt
     1501 aatcaccgat aatggcggct tcttccccca cggtttgctg cctgcgctgg tgttgattca
     1561 gggcgtagtg tttgcttttg cctccattga aatggtgggt acagctgccg gagaatgtaa
     1621 agatccgcag accatggtgc ctaaagccat taacagtgtg atttggcgta ttggcctgtt
     1681 ttacgtcggc tccgtggtgt tgctggttat gctattgccg tggagcgcgt atcaggcggg
     1741 gcaaagtccg ttcgtgacgt ttttctctaa actgggtgtg ccatatatcg gcagcattat
     1801 gaatattgtg gtgctgaccg ctgccctctc cagcctgaac tcaggtctgt actgcaccgg
     1861 acgtattctg cgctcaatgg cgatgggcgg ttccgcaccg agttttatgg cgaaaatgag
     1921 tcgtcagcat gtgccgtatg ccgggattct ggcgacacta gttgtgtatg tcgtcggcgt
     1981 attcctcaac tatctggtgc cgtcgcgcgt atttgagatt gtgttgaact tcgcgtcgct
     2041 gggaatcatc gcttcatggg cgtttatcat cgtgtgccag atgcgcctgc gtaaagcaat
     2101 taaacaaggc aaagcagcgg atgtcagttt taaactgcct ggcgcgccct tcacttcctg
     2161 gctgacatta ctgtttttac tgagtgtcct ggtgctgatg gcgttcgatt acccgaacgg
     2221 gacttatact atcgcggcgc tgccgattat cggtattctg ctggttatag gctggtttgg
     2281 tgtgcgcaaa cgcgttgctg aaattcacag cactgcgcca gtcgtcgagg aagatgaaga
     2341 aaaacaggaa attgtgttta agcctgaaac ggcgagctaa ttgcaaaatt gcccggctac
     2401 gtgccgggca aatttagatt actgacgaag gtgtgccgca tgcggcgtga taaaagtcag
     2461 gcaagtctct ccgttttcac cctcgccaca tctattacag cgcaatacga atcacatcgt
     2521 ccggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgtttg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactga ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgcggggtta aacagcacag ccagtgattc tggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc ggctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctaa tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagcgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctctc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ttcgccagtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcttg ggtcacttcc agagtaaccg ggtcaagacg ataaacaaca ccgcctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc aaagcgcgtt ttcttgctgg ctatatgcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcagacgc gatgaaaaca
     3541 gatgacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 tctcaaatgt ggagcatttc ttgccgttac gactacaatt ttctcaatta cttaacgtat
     3721 cgtgccgttt cgttttccat aaatttacaa ttttattacc atgacggcgt tttcccattc
     3781 ttaacctggc ttttttcatg aagattgttt tcgttcgaca gaccgttttg cccgcactgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac catgattgtc agtgccgcgc
     3901 cgcaggtggt ttcagaactg gatacccctg cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccagcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgc ggatttggct
     4081 cccgctccac ttacggtatt cgcggtattc gcctgtatgt ggacggtatt cccgccacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttttct gccctgtatg gcaacgcgtc tggcggtgta atgaatgtca
     4261 ccacccagac cggacaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaacgg gcgcaacggg agacggcaca caacccggcg
     4381 atgttgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagcc aatgccaaac tgggcgtacg cattgatgaa gccagcaaat
     4501 taagcctgat tttcaatagt gtggatatca aagcagatga cccaggtggg ctaaccaaag
     4561 cagaatggaa ggcgaatcca caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgaacgtag cctgagttcg cgggatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atacccatgg
     4741 caccacaact taacccgtca catgcgggcg gcgtgattac cctgcaacgc cattaccagg
     4801 gaatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttc cgcctgaata
     4921 gcggcatgcc ggagtacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacatcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga ccattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc ataaatggct acctgccggt tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgaggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgtgctgatg ggcaaagcgg tatgaactta ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gtttcagacc gacactgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gccggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcaggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaat gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggttggtat gcaggcacgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggcaaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tatttggtcg tgtcgataat ttattcgata aacaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaaccttcgc ccggacgaaa ttatggtgtc ggcatgaata
     5881 ttgcgtggag atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggccccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgcaaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttccc cacttccgga actgtaaatg cttgtgcagg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttaatgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt catttttccc attccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgtctgc acggcagaag gagttcacct
     6661 ggtgtattaa agtgaataat cataaaccac ccacctttgt tgttattagc agcagaacaa
     6721 tgacgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgcgacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccat cagtaatatc
     6901 ttcgtggtag tggattttat cctctttcac ccattgcccc atctccttct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaacccttgc aagcgaatac gttttttcag
     7021 caccgttgcc atcaacaggg gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aagccacaaa cgggaatgcg cgcagatgta ttaagtaatg gtagcactgc
     7141 atcaaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 caccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcccagcac
     7261 ctcgatagca tggcggcatt tttccgcgcc accggcaacc cccaccaccc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagcagcccc atataagcgg taaagcctgg
     7441 catccccagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcctg ccatccactg tagcccagca cccagtcgcc
     7561 aggctgataa tcaggatgat tcgactccac aacacggctc accgtgccgc cgaccatcac
     7621 gccgccaata tcaacaggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcaccgg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg ccccataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcacggga ttggttttgt ttgcagccgt cacatcccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgattgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggttga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctggt gagtgaagaa gatggcgtag tgacgggata tgcctcgttt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgggattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgctg
     8401 catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgctttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaacacgctg atggtgcgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacccg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctaattgg cggtctgatg ctggatatct
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcagaggat ggtgccgcct
     9001 ttggttaact gttcgtggcg cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttaacc tcttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatatgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttcacc agtttgagaa aaaatcatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcac
     9781 gcagcacgta ctgtcagaga aatcaactct ctgacagtac gcaagatctt ctcatactct
     9841 ccaggtttac aacattatcc ttaatattta cttctttttg ctggcatcta cattgaaatg
     9901 agtagaaaac atccccctac tgtcgtctat acaaacagct tactgattca aacggaatct
     9961 tgttttcaca tttttctatc atgaatactt tatacgcgcg ggagtctata aatgctttat
    10021 actttcaagg aataacgtaa atataaaaag atggcgtaaa gtatatgcat ggcaggatgt
    10081 ttgcaacctc cttgatgacg ctgccccatt gaaattgcaa accccgccct ggttatcttg
    10141 cccatttatc atgcaaaaca gataattata ggcctctgaa tacctcagaa agattgaatt
    10201 ttttatagtc cctct
//
LOCUS       DAZFVO020000168        10189 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFVO020000168 DAZFVO020000000
VERSION     DAZFVO020000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.169"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHKNB_RS20795"
                     /old_locus_tag="pgaptmp_004159"
     CDS             complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHKNB_RS20795"
                     /old_locus_tag="pgaptmp_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGU4197563.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(923..2353)
                     /locus_tag="ACHKNB_RS20800"
                     /old_locus_tag="pgaptmp_004160"
     CDS             complement(923..2353)
                     /locus_tag="ACHKNB_RS20800"
                     /old_locus_tag="pgaptmp_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGU4197564.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2632..3585
                     /gene="tal"
                     /locus_tag="ACHKNB_RS20805"
                     /old_locus_tag="pgaptmp_004161"
     CDS             2632..3585
                     /gene="tal"
                     /locus_tag="ACHKNB_RS20805"
                     /old_locus_tag="pgaptmp_004161"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HGU4197565.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3700..4287
                     /gene="mog"
                     /locus_tag="ACHKNB_RS20810"
                     /old_locus_tag="pgaptmp_004162"
     CDS             3700..4287
                     /gene="mog"
                     /locus_tag="ACHKNB_RS20810"
                     /old_locus_tag="pgaptmp_004162"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HGU4197566.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHKNB_RS20815"
                     /old_locus_tag="pgaptmp_004163"
     CDS             complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHKNB_RS20815"
                     /old_locus_tag="pgaptmp_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HGU4197567.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHKNB_RS20820"
                     /old_locus_tag="pgaptmp_004164"
     CDS             complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHKNB_RS20820"
                     /old_locus_tag="pgaptmp_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HGU4197568.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5776..6180)
                     /locus_tag="ACHKNB_RS20825"
                     /old_locus_tag="pgaptmp_004165"
     CDS             complement(5776..6180)
                     /locus_tag="ACHKNB_RS20825"
                     /old_locus_tag="pgaptmp_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HGU4197569.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHKNB_RS20830"
                     /old_locus_tag="pgaptmp_004166"
     CDS             6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHKNB_RS20830"
                     /old_locus_tag="pgaptmp_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGU4197570.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHKNB_RS20835"
                     /old_locus_tag="pgaptmp_004167"
     CDS             8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHKNB_RS20835"
                     /old_locus_tag="pgaptmp_004167"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGU4197571.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHKNB_RS20840"
                     /old_locus_tag="pgaptmp_004168"
     CDS             complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHKNB_RS20840"
                     /old_locus_tag="pgaptmp_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HGU4197572.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2665 a         2554 c         2637 g         2333 t
ORIGIN      
        1 tctgcaatat attgaatctg catgcttttg taggccggat aaggcgttca cgccgcatcc
       61 ggcattgact acaaacttaa cgctgctcgt agcgtttaaa caccagttcg ccattgctgg
      121 aggaagcttc atcaaagaag taaccttcgc tattaaaacc agtcagttgc tctggtttgg
      181 tcagccgatt ttcaataata aaacgactca tcagaccgcg tgctttctta gcgtagaagc
      241 tgattatctt aaatttgccg ttcttctcat cgaggaacac cggcttgata atctcggcat
      301 tcaatttctt cggcttcacc gatttaaaat actcatctga cgccagatta atcaccacat
      361 tatcgccttg tgctgcgagc gcctcgttca gcttgttggt gatgatatct ccccagaatt
      421 gatacagatc tttccctcgg gcattctcaa gacggatccc catttccaga cgataaggct
      481 gcattaaatc gagcgggcgc agtacgccat acaagccgga aagcattcgc aaatgctgtt
      541 gggcaaaatc gaaatcgtct tcgctgaagg tttcggcctg caagccggtg tagacatcac
      601 ctttaaacgc tagaatcgcc tggcgggcat tctccggcgt gaaatctggc tgccagtcat
      661 gaaagcgagc ggcgttgata cccgccagtt tgtcgctgat gcgcatcagc gtgctaatct
      721 gcggaggcgt cagtttccgc gcttcatgga tcaactgctg ggaattgtct aacagctccg
      781 gcagcgtata gcgcgtggtg gtcaacgggc tttggtaatc aagcgttttc gcaggtgaaa
      841 taagaatcag catatccagt ccttgcagga aatttatgcc gactttagca aaaaaagaga
      901 atgagttgat cgatagttgt aattactcct gcgaaacatc atcccacgcg tccggagaaa
      961 gctggcgacc gatatccgga taacgcaacg gatcaaacac cgggcgcacg ccgagtttac
     1021 gctggcgtag ataatcactg gcaatggtat gaaccacagg cgagagcagt aaaatggcgg
     1081 tcaaattggt aatagccatg caggccatta tgatatctgc cagttgccac atcagcggaa
     1141 ggcttagcaa ggtgccgccg atgaccgttg cgaaggtgca gatccgcaaa caccagatcg
     1201 ctttagggtt gttcaggcgt aaaaagaaga gattgttttc ggcataaatg tagttggcaa
     1261 cgatggagct gaaggcaaac agaataacca cgagggtaac aaactcagca ccccaggaac
     1321 ccattagcac ccgcatcgcc ttctggataa gctgaatacc ttccagcggc atgtaggttg
     1381 tgtcgttacc cgccagtaat atcagcatgg cgcttgccgt acagatgacc agggtgtcga
     1441 taaaaatgcc aatcatctgg acaatccctt gcgctgccgg atgcggaggc caggacgccg
     1501 ctgccgctgc cgcgtttggc gtcgaaccca ttcccgcctc attggaaaac atactgcgct
     1561 gaaaaccgtt agtaatcgcc tggcttaagg tatatcccgc cgcgccgcct gccgcttcct
     1621 gccagccaaa agcactctca aaaatagacc aaatgacgtg gggaagttgc ccgatattta
     1681 ttacgcaaat caccatgctg gtcagcaccc agattatcgc catcaacggg acaaacccct
     1741 gcatgagccg ggcgacgcca tgaagaccgc gagtgattgc cagcagagca aagacagcga
     1801 gaataatgcc tgtcaccagc ggaggaaaat caaaagaaaa acttagggcg cgggcaacgg
     1861 cgttcgcctg aactccgctg aaaattatgc cataggcgat gagcaaaaag acggcgaaca
     1921 gaacgcccat ccaacgcatc cccagcccgc gcgccatata ccatgccggt ccgccacgaa
     1981 actgtccatt gacgtcacgt tctttataaa gttgtgccag agaacattcg gcaaacgagg
     2041 tcgccatgcc gataaacgcg gcaacccaca tccaaaagac ggctccaggt ccaccggcgg
     2101 taattgccag cgcaacgccg gccaggttgc cgctacccac gcgcgccgca agactggtac
     2161 acaatgactg aaatgaggtt aaaccgcctg gctgtggatg aatgctattt ttaagacttt
     2221 tgccaaactg gcggatgtag cgaaactgca caaatccggt gcgaaaagtg aaccaacaac
     2281 ctgcgccgaa gagcaggtaa atcattaccg atccccaaag gacgctgtta ataaaggaga
     2341 aaaaatctgg catgcatatc cctcttattg ccggtcgcga tgactttcct gtgtaaacgt
     2401 taccaattgt ttaagaagta tatacgctac gaggtacttg ataacttctg cgtagcatac
     2461 atgaggtttt gtataaaaat ggcgggcgat atcaacgcag tgtcagaaat ccgaaacagt
     2521 ctcgcctggc gataaccgtc ttgtcggcgg ttgcgctgac gttgcgtcgt gatatcatca
     2581 gggcagaccg gttacatccc cctaacaagc tgtttaaaga gaaatactat catgacggac
     2641 aaattgacct cccttcgtca gtacaccacc gtagtggccg acactgggga catcgcggca
     2701 atgaagctgt atcaaccgca ggatgccaca accaaccctt ctctcattct taacgcagcg
     2761 cagattccgg aataccgtaa gttgattgat gatgctgtcg cctgggcgaa acagcagagc
     2821 aacgatcgcg cgcagcagat cgtggacgcg accgacaaac tggcagtaaa tattggtctg
     2881 gaaatcctga aactggttcc gggtcgtatc tcaactgaag ttgatgcgcg tctttcctat
     2941 gacaccgaag cgtcaattgc gaaagcaaaa cgcctgatca aactctacaa cgatgctggt
     3001 attagcaacg atcgtattct gatcaaactg gcttctacct ggcagggtat ccgtgctgca
     3061 gaacagctgg aaaaagaagg catcaactgt aacctgaccc tgctgttctc cttcgctcag
     3121 gctcgtgctt gtgcggaagc aggcgtgttc ctgatctcgc catttgttgg ccgtattctt
     3181 gactggtaca aagcgaatac tgataagaaa gagtacgctc cggcagaaga tccgggcgtg
     3241 gtttctgtat ctgaaatcta ccagtactac aaagagcacg gttatgaaac cgtggttatg
     3301 ggcgcaagct tccgtaacat cggcgaaatt ctggaactgg caggctgcga ccgtcttacc
     3361 atcgcaccta cactgctgaa agagctggct gagagcgaag gggctatcga acgtaaactg
     3421 tcttacaccg gcgaagtgaa agcgcgtccg gcgcgtatca ctgagtccga gttcctgtgg
     3481 cagcacaacc aggatccaat ggcagtagat aaactggcgg aaggtatccg taagtttgct
     3541 attgaccagg aaaaactgga aaaaatgatc ggcgatctgc tgtaatcatt cttagcgtga
     3601 ccgggaagtc ggtcacgcta cctcttctga agcctgtctg tcactccctt cgcagtgtat
     3661 cattctgttt aacgagactg tttaaacgga aaaatcttga tgaatacttt acgtattggc
     3721 ttagtttcca tctctgatcg cgcatccagc ggcgtttatc aggataaagg catccctgcg
     3781 ctggaagaat ggctgacatc ggcgctaacc acgccgtttg aactggaaac ccgcttaatc
     3841 cccgatgagc aggcgatcat cgagcaaacg ttgtgtgagc tagtggatga aatgagttgc
     3901 catctggtgc tcaccacggg cggaactggc ccggcgcgtc gtgacgtaac gcccgatgcg
     3961 acgctggcag tagcggaccg cgagatgcct ggctttggtg aacagatgcg ccagataagc
     4021 ctgcattttg taccaactgc gatcctttcg cgtcaggttg gtgtgattcg caaacaggcg
     4081 ctgatcctta acttacccgg tcagccgaag tctattaaag agacgctgga aggtgtgaag
     4141 gacgctgcgg gtaacgttgt ggtacacggt atttttgcca gcgtaccgta ctgcattcag
     4201 ttgctggaag ggccatacgt tgaaacggca ccggaagtgg ttgcagcatt cagaccgaag
     4261 agtgcaagac gcgacgttag cgaataaaaa aatccccccg agcgggggga tctcaaaaca
     4321 attagtggga ttcaccaatc ggcagaacgg tgcgaccaaa ctgctcgttc agtacttcac
     4381 ccatcgccag atagattgcg ctggcaccgc agatcagccc aatccagccg gcaaagtgga
     4441 tgattgcggc gttaccggca atgttaccga tcgccagcag ggcaaacagc acggtcaggc
     4501 taaagaaaac gaattgcaga acgcgtgcgc ctttcagcgt gccgaagaac ataaacagcg
     4561 taaatacgcc ccacagaccc aggtagacac caaggaactg tgcatttggc gcatcggtca
     4621 gacccagttt cggcatcagc agaatcgcaa ccagcgtcag ccagaaagaa ccgtaagagg
     4681 tgaatgcggt taaaccgaaa gtgttgcctt ttttgtactc cagcagacca gcaaaaattt
     4741 gcgcgatgcc gccgtagaaa atgcccatgg caagaataat accgtccaga gcgaaataac
     4801 ccacgttgtg caggttaagc agaatggtgg tcatgccgaa gcccatcagg cccagcggtg
     4861 ccggattagc caacttagtg ttgcccataa ttcctcaaaa atcatcatcg aatgaatggt
     4921 gaaataattt ccctgaataa ctgtagtgtt ttcagggcgc ggcataataa tcagccagtg
     4981 gggcagtgtc tacgatcttt tgaggggaaa atgaaaattt tccccggttt acggtatcag
     5041 acctgagtgg cgctaaccat ccggcgcagg caggcgattt gcagtacggc tggaatcgtc
     5101 acgcgatagg cgctgccgct gaccgcttta accccattta gtgccgcacc tacagggcct
     5161 cccagccccg cgccgcgcag caaaccatgc ccaagtacgc tcattgctgc gtgggtgcgt
     5221 aaaatgcggg tcagttggct ggaaagcaaa tgcgacacgc cttttgccaa taatttgtct
     5281 ttcatcagca gcggcagcag ctcttccagc tcattcaccc tcgcatcgac cgcgtgcaga
     5341 aactcctgtt tatgttcctc gtccatattc ttccaggtgt tacgcagaaa ttgttccagt
     5401 aactgttgct caatctcaaa cgtagacatc tctttgtcgg ctttcagctt caatcgtttt
     5461 gaaacatcga gcaaaatggc ccgatacaat ttaccgtgtc cgcgcagttt gttggcgata
     5521 ctatcgccac caaaatgctg taattctcca gcaatcagct gccagttgcg gcgatgttgc
     5581 tcgggatgcc cttccatcga tttaaacagt tcgttgcgca tcagtacgct ggagaggcga
     5641 gttttgcctt tttcattatg ggtgagcaat cgggcgaaat ttgccaactg ttcctcactg
     5701 caatgctgga gaaaatccag atctgaatca ttcaggtaat taacattcat tttttgtggc
     5761 ttctatattc tggcgttagt cgtcgccgat aattttcagc gtggccatat ccgatgagtt
     5821 caccgtatga ccggaaaagg tgatttttga gacgcagcgt ttattgtcgt tatcgctgtt
     5881 aatgttgatc cagtcagtgg tttgcccttc ttttatttct gaaggaatat tcaggctctg
     5941 actggcgcta cgggcggctt tgaaataaac cgatgcaccg cttaactgta aatcgccatg
     6001 gtcggcagag agttgtatgc gtttaacaat tcggcaaaca ggaagtttca gcgccagatc
     6061 gttggtttcg ttacgcggca ttgcaatgac gccgaggatt ttatggtcgt ttgcctgcgc
     6121 cgtgcagcac agcatcagac taatcgccag gctggcggaa atcgtaaaaa cggatttcat
     6181 aaggattctc ttagtgggga gaggtagggg gatggatacc cactagttta ctgctgataa
     6241 agagaagatt caggtgcgta atgttttctt tttattacaa ttttttgatg aatgtcttgg
     6301 ctgcgattca ttctttatat gaataaaatt gctgtcagtt tcacgtcttg tcctgctata
     6361 tcgcgaaatt tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg
     6421 ttacgacccc atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc
     6481 agttgaaacc agacgtttcg cccctattac agactcacaa ccacatgatg accgaatata
     6541 tagtggagac gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg
     6601 tgtagcgatt atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac
     6661 cacgccttct atcattgcct atacccagga tggtgaaact ctggttggtc agccggctaa
     6721 acgtcaggca gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg
     6781 ccgcttccag gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc
     6841 tgctgataac ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat
     6901 ctctgctgaa gtgctgaaaa aaatgaagaa aaccgctgaa gattacttgg gtgaaccggt
     6961 aactgaagct gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa
     7021 agacgcaggc cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc
     7081 tgcgctggct tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct
     7141 gggtggtggt actttcgata tttctattat cgaaatcgac gaagttgacg gcgaaaaaac
     7201 cttcgaagtt ctggctacca acggtgatac ccacctgggt ggtgaagact tcgacagccg
     7261 tctgatcaac tacctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga
     7321 tccgctggca atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc
     7381 cgctcagcag accgacgtta acctgccgta catcactgca gatgcgaccg gtccgaaaca
     7441 catgaacatc aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc tggtaaaccg
     7501 ttccattgag ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga
     7561 cgacgttatc ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga
     7621 gttctttggt aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc
     7681 tgctgttcag ggtggtgttc tgactggtga cgtgaaagac gtactgctgc tggacgttac
     7741 cccgctgtct ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa
     7801 caccactatc ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc
     7861 ggtaaccatc catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg
     7921 tcagttcaac ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac
     7981 cttcgatatc gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa
     8041 agagcagaag atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat
     8101 ggtacgcgac gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac
     8161 tcgcaaccag ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga
     8221 caaactgccg gctgacgaca aaactgctat cgagtctgca ctgactgcac tggaaactgc
     8281 tctgaaaggt gaagacaaag ccgctatcga agcgaaaatg caggagctgg cacaggtttc
     8341 ccagaaactg atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga
     8401 tgcttctgca aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa
     8461 agacaaaaaa taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt
     8521 cctctccgcc cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt
     8581 acgagatttt aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac
     8641 gcctggccat gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat
     8701 ttaaagagat caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg
     8761 atcagtatgg tcatgctgcg tttgagcaag gtggtatggg cggcggcggt tttggcggcg
     8821 gcgcagactt cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg
     8881 gtcgtcaacg tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag
     8941 aagctgtacg tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt
     9001 gccacggtag cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt
     9061 ctggtcaggt gcagatgcgt cagggtttct ttgccgtgca gcagacctgt ccacactgtc
     9121 agggtcgcgg tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg
     9181 agcgcagcaa aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc
     9241 gtcttgcggg cgaaggtgaa gcgggcgagc atggcgcacc ggcaggcgat ctgtacgttc
     9301 aggttcaggt taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag
     9361 tcccgatcaa cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg
     9421 gtcgcgtcaa actgaaagtg cctggcgaaa cccagactgg taagctgttc cgtatgcgcg
     9481 gtaaaggcgt caagtctgtc cgcggtggcg cacagggtga tttactgtgc cgcgttgtcg
     9541 tcgaaacacc ggtaggcctg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa
     9601 gcttcggtgg cccaaccggc gagcacaaca gcccgcgctc aaagagcttc tttgatggtg
     9661 tgaagaagtt ttttgacgac ctgacccgct aacctcccca aaagcctgcc cgtgggcagg
     9721 cctgggtaaa aatagggtgc gttgaagata tgcgagcacc tgtaaagtgg cggggatcac
     9781 tccccgccgt tgctcttact cggattcgta agccgtgaaa acagcaacct ccgtctggcc
     9841 agttcggatg tgaacctcac agaggtcttt tctcgttacc agcgccgcca ctacggcggt
     9901 gatacagatg acgatcaggg cgacaatcat cgccttatgc tgcttcattg ctctcttctc
     9961 cttgacctta cggtcagtaa gaggcactct acatgtgttc agcatatagg gggcctcggg
    10021 ttgatggtaa aatatcactc ggggcttttc tctatctgcc gttcagctaa tgcctgagac
    10081 agacagcctc aagcacccgc cgctattata tcgctctctt taacccattc tgttttatcg
    10141 attctaatcc tgaagacgcc tcgcattttt atggcgtaat tttttaatg
//
LOCUS       DAZFVO020000169        10165 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFVO020000169 DAZFVO020000000
VERSION     DAZFVO020000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.170"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..863
                     /locus_tag="ACHKNB_RS20845"
                     /old_locus_tag="pgaptmp_004169"
     CDS             <1..863
                     /locus_tag="ACHKNB_RS20845"
                     /old_locus_tag="pgaptmp_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HGU4197573.1"
                     /translation="TTATPTTAVSSQSNALPPRPLLQATQLPALVMTGKPGQSSVVYL
                     REARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQL
                     YGHMDWNRKILTVTTTAAPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVS
                     ASRAQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGTTLKDGLARWTS
                     SEKCPHGTWTLRWETPVNYSIDAPLVFTGSFHQALEQLFALYTPVEKPLYARTSTAQC
                     LLRVTDRPQE"
     gene            868..1305
                     /gene="pilM"
                     /locus_tag="ACHKNB_RS20850"
                     /old_locus_tag="pgaptmp_004170"
     CDS             868..1305
                     /gene="pilM"
                     /locus_tag="ACHKNB_RS20850"
                     /old_locus_tag="pgaptmp_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HGU4197574.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1336..2955
                     /locus_tag="ACHKNB_RS20855"
                     /old_locus_tag="pgaptmp_004171"
     CDS             1336..2955
                     /locus_tag="ACHKNB_RS20855"
                     /old_locus_tag="pgaptmp_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HGU4197575.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLAYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2976..4271
                     /gene="pilO2"
                     /locus_tag="ACHKNB_RS20860"
                     /old_locus_tag="pgaptmp_004172"
     CDS             2976..4271
                     /gene="pilO2"
                     /locus_tag="ACHKNB_RS20860"
                     /old_locus_tag="pgaptmp_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HGU4197576.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4261..4713
                     /gene="pilP"
                     /locus_tag="ACHKNB_RS20865"
                     /old_locus_tag="pgaptmp_004173"
     CDS             4261..4713
                     /gene="pilP"
                     /locus_tag="ACHKNB_RS20865"
                     /old_locus_tag="pgaptmp_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HGU4197577.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEAGRQQESISAVTTNVQALPSPRLTAIFGVHPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKILRP"
     gene            4798..6306
                     /locus_tag="ACHKNB_RS20870"
                     /old_locus_tag="pgaptmp_004174"
     CDS             4798..6306
                     /locus_tag="ACHKNB_RS20870"
                     /old_locus_tag="pgaptmp_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HGU4197578.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARLRQDATTTGDENAINPIQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSILGYHPDQE
                     CTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTTRGWVDSIKSALRLDPDSILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASSRTKDERHLVETFCNADAVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6308..7408
                     /locus_tag="ACHKNB_RS20875"
                     /old_locus_tag="pgaptmp_004175"
     CDS             6308..7408
                     /locus_tag="ACHKNB_RS20875"
                     /old_locus_tag="pgaptmp_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HGU4197579.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVENMYNAHTRLIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWISGAIITLVIIIRFSLSRYTGPGRRLL
                     DKLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKNSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7482..8018
                     /locus_tag="ACHKNB_RS20880"
                     /old_locus_tag="pgaptmp_004176"
     CDS             7482..8018
                     /locus_tag="ACHKNB_RS20880"
                     /old_locus_tag="pgaptmp_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HGU4197580.1"
                     /translation="MNFRARLSSYFSLKKDKEDDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNTVD
                     PVTVCSSDKNNIKLETNS"
     gene            8072..8545
                     /locus_tag="ACHKNB_RS20885"
                     /old_locus_tag="pgaptmp_004177"
     CDS             8072..8545
                     /locus_tag="ACHKNB_RS20885"
                     /old_locus_tag="pgaptmp_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HGU4197581.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8564..9199
                     /locus_tag="ACHKNB_RS20890"
                     /old_locus_tag="pgaptmp_004178"
     CDS             8564..9199
                     /locus_tag="ACHKNB_RS20890"
                     /old_locus_tag="pgaptmp_004178"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HGU4197582.1"
                     /translation="MFLIILLVISATLTIQLLAIRVAGHYLMQVHTLRLPRHLSLLCV
                     VLALTATITHIIRGTDITGLCFLLLMAGCMTLMTLTDATSCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAATGTMVCMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9204..>10165
                     /gene="pilV"
                     /locus_tag="ACHKNB_RS20895"
                     /old_locus_tag="pgaptmp_004179"
     CDS             9204..>10165
                     /gene="pilV"
                     /locus_tag="ACHKNB_RS20895"
                     /old_locus_tag="pgaptmp_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HGU4197583.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNARLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWTDKP
                     GDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPGRPELNQMHTAINMGGNDINNAGNL
                     NGQKATVKGDITSEDGWLITRNNKGWMNTTHGGGFTMTDSQWIRAVNNKGITTDGEIK
                     GGKVSGGTIRSDGRLSTGEYLQLEKTATAGTSCSPDGLVGRDSKG"
BASE COUNT         2600 a         2825 c         2543 g         2197 t
ORIGIN      
        1 caacaacagc gacgcccaca accgcagtca gcagccagag taacgcactg cctccccgcc
       61 cgctccttca ggccacacag ctgcccgcac tggttatgac cggtaaaccc ggtcagtcat
      121 ccgtcgttta tttacgtgag gcacgtcagc gctccgtcag tgactggata cgccagttgc
      181 tgccacccgg ctggcagttc cggcaacaga acgctgccat accggcactg aacaaacgcc
      241 tccccgtctg gtccgttaat gaccagtggc accgggcact gaatactctg ctgaccgggc
      301 agcaactgta cggacatatg gactggaaca ggaaaatact gactgtcacg accactgcag
      361 caccgcctgt tgacctcacg gcaccgcagg gttcacagaa aacagcggac acaccacgta
      421 atccgttcag gagcacaacc ggaacggctg cagaccaggc acaggtacca cctgccgtca
      481 gcgcttcccg ggctcagagc acaacaggga catccggtac atctgcgccc cgcacaacca
      541 cgccagcccc ggtggtcacg aaaccggtag caccaccaca gaaggtcaca ccgccacggc
      601 agtggaaaat ggaaaaaggg accacgctga aggacggact ggcccgctgg acgtcgtccg
      661 aaaaatgccc ccacggcacc tggacgctgc gctgggaaac gcccgttaac tacagtatcg
      721 atgcacctct ggttttcacc ggcagtttcc accaggcgct ggaacagctc ttcgcactct
      781 acaccccggt ggaaaaaccg ctgtatgccc ggaccagcac agcacaatgt ctgctgcggg
      841 tgacagatcg tccacaggaa taaccttatg ggctggttca tcgtgttcat catggtcatc
      901 acgaccacag gcaatttctg gttcaccagt cagctctccc gcagcgaaca ccgtcatcag
      961 gccgcagaaa atacacaaca ggcagcaacg ttcattcgtt atatgaacgc catcaatgat
     1021 tacctgcatc agcatccgga acgacggaca gccggagggc ggctgacatc cgctcagctc
     1081 gggattcccg ggacggatac cgtcagtcat atcatcagcc agcaacgtgt ttttgtctgg
     1141 gctacagaaa ccccgggcct gatggccgcg ctcagggaac agagtaatga ctctgctctg
     1201 ctcggcaggg tggaaaacgg ccgactgctg gacactgccg gcagagcgct ttccgtcaca
     1261 cttccgtcag tgatccccga tcacgtcatc ctctggatga attagtcacg aatctgaata
     1321 aatcacggat ctcccatgaa taaaaaaatt cttgccatta tgattgccgc cgctctgtta
     1381 cagggctgtg ccagttacag acagcttgag agcacaagaa aggaagccgt tgcggaacaa
     1441 caacgggcac gggaaatgat gtctgatctg cgaacccgga cgccggttgt taaggaatcc
     1501 tcacgacaat ggatcaatcc ccgtccggtc atggataaaa acaccggcaa aaccgttccc
     1561 ggttgtccca ttgtcattca tacccgtcag agcatcaccc ttcagcaggt ggttcagcgg
     1621 atcacggaaa gctgccatat tccggtacgg atcacacctg atgtactggc atacctgaat
     1681 acatccacaa ccggcagcac acaacaaatc cagggcgcgt tacccccacc ggatgcgagc
     1741 ggcatggttc cccttgccgc cattggcgca tccgacacgc caccatcccg tttgccggga
     1801 aataccgcag cactgtctga actgaatgcc tccggcctga cgaccctgct gaatgcggtc
     1861 tcctcccgtc tcggtatatc ctggcgctat gacggctcgc atatcatctt ctattatctc
     1921 gagacacaga ccttccccat cacctatatg gacagtcagg tggcctataa cgccactgtg
     1981 gtcagcggca ccatgtcctc aaacggctca tcaggaggca gtgcatcaga tgccatttcc
     2041 ggtgatgcca gcaataccca gaccaccacc gtggatatga agtctgcgct gtatcaggat
     2101 gtgaaaaatg cggtcacatc aatgctgacc ccgggtatcg gacgcatgtt catgtccacg
     2161 ggctttatca ccgtcacaga taatccacag gttctggaaa cggtccgggc attcattgaa
     2221 aaacgcaatg aagagatgaa acgtcaggtg gtgctgaatg tggaaattct gagtatccgg
     2281 aaaacccgca atgagcaggc cggtatcgac tggaacgcag tcttcagcga cagaacgctg
     2341 ggactgagtc tcggcagcac attcaccagt gcagccagtg atgccatcac aggtggtgtt
     2401 tccattgtgg acggtaaact caccggttca aaagccttcc tgaaagcatt gtcttcccag
     2461 ggggatgtca gtgtggtgac ccagaactct gccgtcacca aaaacctgac gccggtgcca
     2521 atgcagatcg ccaatcagca gagttacata gagagtgtga cgacggatac cactgccaat
     2581 gtcggctcat ccacctccct gaatgcggcc accatcacca ccggttttaa catgaccctt
     2641 ctgccgttca ttctcccgga ctcacagaca ctgcagttgc tctattccat gagtctctcc
     2701 gacaaaccgg ttattgagaa ttatgagagc ggtggaagta aagcccagct gccgaatgtg
     2761 gatctgaaaa ccatcaatca gaccgtggat ctgaaatccg ggcagacggt cattatcagt
     2821 ggcttccagc agtcaggacg tcgcagcgga aaacagggtg taggtacgcc gggattcttc
     2881 ggtcttggcg gtgggatcaa cagtgaaaac gacgacacca ttctcgtggt gctgatcacg
     2941 cccaatatca tctgatttgc acaaggagga cgatcgtgac ggatatttca ccgacagggc
     3001 ggcaggtgtt tccgctgacg gcagggcgca atgtgtttct ggccggtctg gaatggaaaa
     3061 cactgccctc acagtaccgg catgcccgcg actttgcccg ggcacagaaa gccgaccttt
     3121 tcctggcctg tcagtatctc agtaatgaag atgccgatac ccacaccatg gtagccactg
     3181 tcagtcgccg catactgccc ggaaaacccc gtcagtgctt ctccctcgcc ctgctgatcc
     3241 tgccactgct tgaacatggc ggctatgcca tcacggaact gacgttaccc ggagaaacac
     3301 cgcgctatag ctttgtttcc gttgtcgacg gtgttctcgt cagtgacctg gtgggaagcg
     3361 gagaagaggt tcgggaagcc cgggatacct ttctttccat caacaccgaa ccgactcagg
     3421 gctggataca ctatgaaccg gcggcatttt cagcaggcga tcagaatcag cacctgccgc
     3481 tggcctccct gactggctct ggtaaacatc cggctgccgc acgcctgaat ccggtatccc
     3541 ggggaccgca gttcatcact gtctctctga tcatcgccct tctcggcact gcatggtacg
     3601 gctggcagta ttatgaacgc tggcagacag aaaaagccgc gcaggcagcg gttgcccgac
     3661 aggccacaga ggaacgaatc actcccccct ggcccggact ccctgaaaca gacgcgttca
     3721 tccggggatg tgctgatata tggacttcac tgccactgtc tgctgccgga tggcgttatt
     3781 cgctggcaga atgctcgact gacggtggca gcggcaatct tcgcgcatcg tacaccaata
     3841 ctgcgggcgc gacagtgact gacttcatcc ggcgcatcag tgatatgaca gacagccgac
     3901 cgtttattgt gatgccggaa ggaaagaccg gcggcattgg cctgccggtc accttccggc
     3961 tgtctgaaaa cgccgtcaca gttgatgcac taccggaaac cgctgtcctg caggaacgac
     4021 tgacctcgct ggctcagtca atgcaactga aactggactg gcaggaagtt aataacagcg
     4081 tcactgatga gaacggacag atcattcagc ccccatggaa agagtatgac cttcagatac
     4141 agaccacact ccctgcacac catctggctg aacgcctgaa agagccttct gtccgtttta
     4201 tctctgtcac acggcaactg gagaacggtc gtttccacta tcaatttaca ggtaaatatt
     4261 atgcccgata aagccaaaac ccttctgttt tcagtcctgc tgttaccggc cacggcaatg
     4321 gccacaaccg gaaatgcaga gcaacagaac acacagcaaa taaggattga acagatacag
     4381 gaagagacag ccctgtatga agccatgctg gcgagagaga agatccgcca tgaactgcga
     4441 acacttgagc agggcgttcc cgcagaggcc ggacgtcagc aggaaagcat ttccgcagtt
     4501 acaacaaatg tacaggcatt accatccccc cgcctgacag caatattcgg tgttcatcct
     4561 cagctgactg cccggataga acttccgggg ggaagcatct ccgatgtgaa ggcgggccag
     4621 aacatccccg gaaccactta tcacgtggaa aaaatcacgc cggacagtgt ggtgctggaa
     4681 cagtctggca ccagaaaaat cctgcgacca tgagaccggc cgccgtaact ggcggctgtc
     4741 aaaaataaaa ggatgaaaga catattttcc gggcaccgct gcccgtaagg aggctttatg
     4801 ctggatccag cagacctgcc gccggatgac attctggatt atgtggccat cgacaccgat
     4861 aacgccggac aactgcggat cagggttgtt gagggaaaaa aacatctgcg tgccgtccag
     4921 gaatatctga cccgccttcg cgcacgctat caggggcgtg tgggagactt tgagttcacc
     4981 acgctggatg tgatagcccg cctcagacag gacgccacaa cgacaggcga tgaaaacgct
     5041 attaacccga tacagcagaa aatgctcggc tatatccgcc attctgcctc ccttggtgcc
     5101 tccgaccttc atatgacgcc agggcgggac aacacagact tcacctatgt ggaagcccgg
     5161 gttcatggcg aactggaagt gctcgatatc ctccggaaag aagaaggtct ggaattactg
     5221 ggggctacct attccggcat gacggatgtc atcaagggca cacaatttga tcccggggtt
     5281 ccacaggacg cacgtctctc tgaacagtac ctgaaactgg ctggcctgtt tggcgcacgc
     5341 tactcccact acccctgcgt gggaggcctc tatgctgttc tccgtctgat caaggatgac
     5401 agccagcaaa tcccgacctt cagtatactg ggttatcacc cggatcagga gtgcaccctg
     5461 cgccgtatcc tgcaacgccc tgaaggcatt atcaccctct ctggccccac cggctcggga
     5521 aaatccacca cgctgagaac cgcctctgcg gcataccttg aacaatacgg cttcaataac
     5581 accggcggta ttcttctgcc acggcgcagg ttgttcacga ttgaatcccc gccggaaggc
     5641 cgtatccccg gagcaattca gacagccgtt atggatacca cccggggctg ggtggactcc
     5701 atcaagtcag cactgcgtct tgatcctgac agcatcctga acggtgaaat ccgcgatcac
     5761 gattccgcca ttacggcaat aaaagccgcc atgaccgggc atctgatgct gaccaccatc
     5821 catgcgaacg acccgattaa catcctcgaa cgccttgaga tggagggtgt tcaggccagg
     5881 atgattgcag acccgcagtt gttcatcgga ctgctcagtc agcgtctggt tcagctgata
     5941 tgtccccact gtcgtcatcc atggaatgac gtagcatcca gtcgtacaaa ggatgagcga
     6001 catctggtgg aaaccttctg taatgctgat gcggtatatc tgagaaacca tgaaggctgt
     6061 ccacactgct ggcgtggtgt cacagggcgt acagtgattg cagaagtcat ctctcctgac
     6121 gccagatttt ttcagttata ccgggaaaaa ggccgtatcg aagccaaaac ctactggcac
     6181 agggaactgg gcggaatgac gcgtaatcag catctgctgg gaaaaatcaa cagcggactg
     6241 gttgaccctc tggcggccca ttacatctct cccattgatg aggacagcta tacgctgctg
     6301 cgctgatatg aaatttctgg aaaaactcaa cagtactgaa ctggatctgt ctgccctgaa
     6361 cagctggaca gccagaaaaa ccttcggtct taagcgtgcc gagttctatg agtccctcgg
     6421 cggaatgata cgtgacggaa cgccgtcact gcgcgccctg gagtttatct gtaatgtcga
     6481 aactgacttt ggcacccgaa aaggaacatc cggcatgtac tttctggcca gagaatgtat
     6541 tgcctccctg aaagccagcg gtatgctgtc gggggccctg cccacatggg ttcccccgga
     6601 agaagcggca ctgatcagga acggtgaaga acgcgggaac atcgccgaag ccctgttcca
     6661 ggtcgcaaaa accgtgaaag cacggcggga aatggtgagc agtctcatca cactctgtct
     6721 ttatcccctt ctgctgcttt cactctgtgt ggagaatatg tacaacgcac atacccgcct
     6781 catccccatt gtcagcgctt ttgcaccgga ggagaaatgg tcattccaga tgaaagtcct
     6841 tgcagccatg tcagagcttg tcatcggcta tggtctgtgg atcagtggtg ccatcatcac
     6901 gcttgtcatc atcatccgct tctcactcag tcgctatacc ggcccgggac gcaggcttct
     6961 tgacaaactg ccaccatggt cgctctatca gacttttcat ggtgtgaatt atctgttcag
     7021 catatcgtca atgctgcaga taaatattcc gctttcacag gcgctgctca gaattgaaaa
     7081 aagtgcagag cataataaat ggttaaagga gagaattcag gcaataagaa aaaatgtatt
     7141 atccggtcag aatctggcca ccgccatgcg caattctgac tatgattttc cttcaaaaat
     7201 atgcatcaat aaacttcttt taaattctga aagggaagac agcgtatctg caatgaatac
     7261 ctatgccgga cagtggctgg aagatgcaaa aaatagcgtc agaaaaagtg gattaattat
     7321 caccggtatt tcaggaggtt ttgtattctt ttttattctt aacatgatta gcgcgatata
     7381 cagtatcagc gatatgctca agcaataata cctgccgtca tactaaaacg cccttcaata
     7441 tactttcatt aacgtaacaa ccattaatac aggagtcata tatgaacttc agagcacgtc
     7501 tgtcttctta tttttcttta aaaaaagata aagaagatga taaaggttta acattattag
     7561 aaattgccgg cgcaatgatt atcatcgcaa tagttgtcgc gctcgcgatg agccagatgt
     7621 ccagcacaat ggcctccagt gacaaccagc gcgaactgaa aaacctgcag acaattgcaa
     7681 ccaaaatgaa gcaatacaag ttccagggaa gatataccgg aacggattat atcaaaaccc
     7741 tgacggaaag cggtgcattg cctgctgata tgattgcagg aggcaacaaa gccaaaaacg
     7801 catggggtgg tgatgtcacg attgctgcca cagcagacaa atatggctac attatcacca
     7861 gtaataatgt tcctaaaaca aactgtgttg agctgattaa cagcctgcgc agttcgtcca
     7921 tatttaccaa aattatgaac acagccccga acacggtgga tcccgttacc gtctgcagta
     7981 gcgataaaaa caatatcaag ctggaaacca acagctaatg ccctgccaca cagcactccc
     8041 tccggggtgc tgtttcccgg agcacataat catgaaatgg ttttatctat catctctttt
     8101 cttattctgt ctcccggcga ctgccgccgt aaacagtaac acactggata gctgctttat
     8161 tcaggcagga aaacgttact ccatcgatcc cggactactg aaagtaattg cccgtaaaga
     8221 aagcagcctg aatccccggg ccatcaatca taacagaaat gccagcggaa aaattatcag
     8281 cacagattat ggcctgatgc agattaattc cacacatatt cccggactga aaagaatggg
     8341 aattatccga agtgaaaatg aactgctcag taatgtttgc ctgaatatac agacaggtgc
     8401 atggatcctt gcccggcatt ttcagcagtg tggtgtgaac tgggagtgtc tcggtagcta
     8461 caacgccgga tttaataaaa ataacactca ccgcagaatg aaatatgccc ggctgattta
     8521 tgccgcatat atgcagggac gttaataacg gagaatacga gccatgttcc tcatcatctt
     8581 actcgtgatc tccgctacgc tgaccataca gttacttgcc atcagagtgg ccggacatta
     8641 tctcatgcag gtgcataccc tccggcttcc acgtcacctc tccctgctct gtgttgttct
     8701 ggcactcacc gccaccatta cgcacatcat ccgggggaca gacattaccg gactttgctt
     8761 tctgttgctg atggcaggct gcatgacact gatgacacta actgacgcaa cctcctgccg
     8821 tcttccccgt cccttcaccc tcacattcct gatgctgggt gtggcgtttc gtttatggca
     8881 gggcgatgct gctattgcgc ttgcctgtgc tctgttctgg tttctcctgt tatctctcct
     8941 gaggcgctac acctcatacc gcaagggagt ggagacgctg ggactggggg acgtgtttct
     9001 gattgcaggt gtggctatgt ggtcatctgc caacagcact ccgtggatca tcggtattgc
     9061 cgccaccggc accatggtct gtatgtcgct gttcccgcgc agtaatacag gggccgctat
     9121 cccgttcggt ccgtttatct gtgcatcact gtacattttc actttttttc ctggtgccat
     9181 ttttcacacc ggagggtaac accatgcaaa aagacgacga taaaggtttt gttctgtttg
     9241 aaattctggc cggactgatt gtgatcggta tcgccacacc aatgatttat tcagaaattg
     9301 aaaactggct gaatgagcag ctttaccaga gtgctgcata tcatgcagat gcctacaata
     9361 ccgcagccag aaactatatt gccgacaaca atgcccgtct gcatagcggc agccttccgg
     9421 caaatttcac ggctgatgat ctgattcgtc agggatatct gaaacagggg tttaaccatt
     9481 ctccgtttgg tcagagttat atcacgggta tccgccgtaa ccagactaca ggacgtcttg
     9541 aagccctgac ctgctcaaca ggggggcaga atatcaaaga agatggtctg cgcagtgtgg
     9601 caggacagct tcccgggctg ggtggttata ttggtaaaaa cggaacagca accggcgcat
     9661 tcggcgcctg gacagacaaa ccgggggatt acggactcac ctgttcggca ggtcacattg
     9721 ccgtcgtcat gacgggtgat gatctgcagg aaagcgacag gttgtatcgt tttcaggtcc
     9781 cggggagacc agagctgaac cagatgcaca ccgccatcaa catgggcgga aatgacatca
     9841 ataacgccgg taacctcaat ggtcagaaag ccacggtgaa aggcgatata accagcgaag
     9901 acggctggct tatcaccaga aacaacaagg ggtggatgaa cacgacgcac ggaggcggtt
     9961 tcaccatgac tgacagccag tggatacgcg ccgtcaataa caaagggatc accaccgatg
    10021 gtgaaatcaa aggaggcaaa gtcagcggtg gaacgatccg gtctgacggt cgtctttcca
    10081 caggtgagta tttacagctg gaaaaaacag ccaccgcagg tacttcctgc tcaccggacg
    10141 ggcttgtagg ccgcgacagt aaagg
//
LOCUS       DAZFVO020000170        10091 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFVO020000170 DAZFVO020000000
VERSION     DAZFVO020000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.171"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1603
                     /gene="speC"
                     /locus_tag="ACHKNB_RS20900"
                     /old_locus_tag="pgaptmp_004180"
     CDS             <1..1603
                     /gene="speC"
                     /locus_tag="ACHKNB_RS20900"
                     /old_locus_tag="pgaptmp_004180"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HGU4197584.1"
                     /translation="SSSNKVVLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPVYLE
                     TARNPYGFIGGIDAHCFEESYLRELITEVAPQRAKEARPFRLAVIQLGTYDGTIYNAR
                     QVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAG
                     FSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSG
                     RNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPG
                     EHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEK
                     CDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYT
                     LRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAE
                     GRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHD
                     GRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1600..2919
                     /gene="potE"
                     /locus_tag="ACHKNB_RS20905"
                     /old_locus_tag="pgaptmp_004181"
     CDS             1600..2919
                     /gene="potE"
                     /locus_tag="ACHKNB_RS20905"
                     /old_locus_tag="pgaptmp_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HGU4197585.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="ACHKNB_RS20910"
                     /old_locus_tag="pgaptmp_004182"
     CDS             complement(2975..4615)
                     /gene="pgm"
                     /locus_tag="ACHKNB_RS20910"
                     /old_locus_tag="pgaptmp_004182"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HGU4197586.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="ACHKNB_RS20915"
                     /old_locus_tag="pgaptmp_004183"
     CDS             complement(4641..5186)
                     /gene="seqA"
                     /locus_tag="ACHKNB_RS20915"
                     /old_locus_tag="pgaptmp_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HGU4197587.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5371..6135
                     /gene="ybfF"
                     /locus_tag="ACHKNB_RS20920"
                     /old_locus_tag="pgaptmp_004184"
     CDS             5371..6135
                     /gene="ybfF"
                     /locus_tag="ACHKNB_RS20920"
                     /old_locus_tag="pgaptmp_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HGU4197588.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6275..6568
                     /gene="ybfE"
                     /locus_tag="ACHKNB_RS20925"
                     /old_locus_tag="pgaptmp_004185"
     CDS             6275..6568
                     /gene="ybfE"
                     /locus_tag="ACHKNB_RS20925"
                     /old_locus_tag="pgaptmp_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HGU4197589.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6708..7238
                     /gene="fldA"
                     /locus_tag="ACHKNB_RS20930"
                     /old_locus_tag="pgaptmp_004186"
     CDS             6708..7238
                     /gene="fldA"
                     /locus_tag="ACHKNB_RS20930"
                     /old_locus_tag="pgaptmp_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HGU4197590.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7448..7534
                     /gene="uof"
                     /locus_tag="ACHKNB_RS20935"
                     /old_locus_tag="pgaptmp_004187"
     CDS             7448..7534
                     /gene="uof"
                     /locus_tag="ACHKNB_RS20935"
                     /old_locus_tag="pgaptmp_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HGU4197591.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7527..7973
                     /gene="fur"
                     /locus_tag="ACHKNB_RS20940"
                     /old_locus_tag="pgaptmp_004188"
     CDS             7527..7973
                     /gene="fur"
                     /locus_tag="ACHKNB_RS20940"
                     /old_locus_tag="pgaptmp_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HGU4197592.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8057..8383)
                     /gene="chiQ"
                     /locus_tag="ACHKNB_RS20945"
                     /old_locus_tag="pgaptmp_004189"
     CDS             complement(8057..8383)
                     /gene="chiQ"
                     /locus_tag="ACHKNB_RS20945"
                     /old_locus_tag="pgaptmp_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HGU4197593.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8433..9839)
                     /gene="chiP"
                     /locus_tag="ACHKNB_RS20950"
                     /old_locus_tag="pgaptmp_004190"
     CDS             complement(8433..9839)
                     /gene="chiP"
                     /locus_tag="ACHKNB_RS20950"
                     /old_locus_tag="pgaptmp_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HGU4197594.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDSTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPNAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2411 a         2517 c         2559 g         2604 t
ORIGIN      
        1 ttcatcttct aataaagtgg tcttaaacgc attgctgacg ccgggcgatc tggtgttgtt
       61 tgaccgcaat aaccataaat ctaaccacca cggtgcgttg ctacaggctg gcgcgacacc
      121 ggtttatctg gaaaccgcac gtaacccgta tggttttatc ggtggtattg acgctcactg
      181 ttttgaagaa agttacctgc gtgagctgat tacagaagtt gcaccgcagc gggcaaaaga
      241 ggctcgtcct ttccgcctcg ccgttattca gctaggcact tacgacggta cgatttataa
      301 cgcccgtcag gtggtggata aaattggtca tctgtgtgac tacattctgt ttgactccgc
      361 atgggttggc tatgagcaat ttatcccgat gatggctgac tgttcgccgc tgttgctgga
      421 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
      481 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
      541 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
      601 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
      661 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
      721 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
      781 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca
      841 ttcttttgaa ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac
      901 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
      961 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     1021 cctcttcctg ctgactccgg cagaagatat ggccaagctt cagcaacttg ttgccctgct
     1081 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat
     1141 ctacaaacag catgaagagc gctacgccgg ttataccctg cgtcagttgt gtcaagaaat
     1201 gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     1261 acacttccca cgcgtaagca tgaatccgca agaagccaac tacgcctatt tacgcggtga
     1321 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     1381 ttatcctccg ggtgtgctgt gcgttgttcc gggtgaaatc tggggtggtg ctgttctgcg
     1441 ttacttcagc gctctggaag aagggatcaa cctgttgcca ggttttgcac cggagcttca
     1501 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     1561 tcgtgatgcg caaagtaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     1621 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     1681 atcatgctgc cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg
     1741 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     1801 aaatcaggtg gtatgggcgg ttatgccgag tatgctttcg gtaagtccgg caattttatg
     1861 gcgaactaca cctacggtgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     1921 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     1981 accatcggag tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     2041 caaatcagta gcattactgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     2101 ggctggttct ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg
     2161 ttcttcagtg cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag
     2221 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     2281 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg
     2341 attgtgccta atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag
     2401 atgttcacac cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     2461 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     2521 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     2581 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     2641 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     2701 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     2761 gcaaactttg ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg
     2821 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     2881 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     2941 gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa
     3001 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     3061 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcgg
     3121 tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg
     3181 tgatcgggtc gcctgcaagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg
     3241 ccgctttttg tgcggaagtc gcggctgcct gcaaacggtt gtagctcggc gcaccaaagc
     3301 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg gtgatttccg
     3361 ccgccagcag acacataatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac
     3421 gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg tcgaacagac
     3481 catcaacaaa ccatttgaaa cctaccggga cttctaccag cttacggccc aagtcattga
     3541 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     3601 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     3661 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     3721 gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag gagcagtcca
     3781 tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt
     3841 taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg
     3901 aaccgcccag cggatcaacg cccagcgtca ggcccgcttt ctgaattgcc gccatatcaa
     3961 cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttt acatgaccgg
     4021 atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg
     4081 cgttggccct gtcttccacc actttggtga cgttggtatc agccgggcca ccatttggcg
     4141 ggttgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt
     4201 ctgccagcgg gccacctttt ttattgtgaa ccaggatggc attggaaatg gcaggtgtcg
     4261 gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     4321 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     4381 cagtgatgcc gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     4441 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     4501 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     4561 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     4621 tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg
     4681 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     4741 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     4801 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     4861 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     4921 ttgaccgctc gcttttgctc tgcgtattca tccgaaagca gaagttcacg cattgcgcga
     4981 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     5041 acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt
     5101 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     5161 tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag
     5221 tcgccaggag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     5281 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     5341 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     5401 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     5461 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     5521 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     5581 cttgttgata ctttggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     5641 ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg
     5701 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     5761 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc
     5821 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     5881 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     5941 atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc
     6001 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     6061 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     6121 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     6181 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     6241 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     6301 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     6361 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     6421 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     6481 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     6541 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     6601 ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg
     6661 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     6721 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     6781 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     6841 aagcttatga cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg
     6901 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     6961 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     7021 tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct
     7081 atcatttcga agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg
     7141 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     7201 ctgaagagtt gcatctcgac gaaattctta atgcctgatg tgatgcggcg tagactcatg
     7261 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     7321 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     7381 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     7441 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     7501 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     7561 gcctgaaagt aacgcttcca cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc
     7621 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     7681 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     7741 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     7801 tttgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     7861 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     7921 actgtgccga aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc
     7981 tgaacgagaa aagccaacct gcgggttggc ttttttatac aagggaaata agaaaagcga
     8041 gagttacagc tctcacttat ttgttattac tgcgtatttc ctgccagacc ttatcacaat
     8101 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca
     8161 gtacgcgtac actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt
     8221 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
     8281 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
     8341 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
     8401 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
     8461 ataaatttca cgtcacgctc atcctggaag atgttaccgt aaccgccgcc ccagcttggg
     8521 atgtcagagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
     8581 ccgtcctgaa tggtgtagac cgcatccagg ctgtaggcag actcttcaat agtccggttt
     8641 ttgtcgtagt acgcattcgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa
     8701 acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca
     8761 ccgaagaaga ccgctttttc gccgttggcg ttgaagtcgg aacggttatc ccaccagata
     8821 tccaggcggc cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc
     8881 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
     8941 taaccaaagg tcaacgcttg cagccaggcg gtgctgtcat aaaggtcgtt gacgctgcga
     9001 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
     9061 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
     9121 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga
     9181 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
     9241 gctttgtatt cgttggtcca catgtaggag aaactcaacg caccagcatc gccgtaatca
     9301 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
     9361 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
     9421 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
     9481 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
     9541 gccatttcaa tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg
     9601 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
     9661 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
     9721 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
     9781 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
     9841 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa
     9901 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
     9961 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    10021 tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagt
    10081 gtgaatttcc a
//
LOCUS       DAZFVO020000171        10020 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFVO020000171 DAZFVO020000000
VERSION     DAZFVO020000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 10020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.172"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..560)
                     /locus_tag="ACHKNB_RS20955"
                     /old_locus_tag="pgaptmp_004191"
     CDS             complement(<1..560)
                     /locus_tag="ACHKNB_RS20955"
                     /old_locus_tag="pgaptmp_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197595.1"
                     /translation="MFSPTILLTLAGGIIMLLFCLFSFLIDFKLTRPIVFMCGGVFIG
                     VGVLGHITFIEILEHCKELATSGNVSRDVLSRLERYHLIFTYILPFISGAIGTNVISD
                     ALLKHQTYERSFSFIQFVRDCAEIFLVLIGLVLGVFCIFFFGVSILVAFTRNLVFLYF
                     PRIQRWVYLKVLKISMLMKNVFRSKP"
     gene            complement(1344..1571)
                     /locus_tag="ACHKNB_RS20960"
                     /old_locus_tag="pgaptmp_004192"
     CDS             complement(1344..1571)
                     /locus_tag="ACHKNB_RS20960"
                     /old_locus_tag="pgaptmp_004192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197596.1"
                     /translation="MTEDDVTPEAQLRFDMMVDQVSVSLTEPFRAQHRQRHPKMLSTT
                     DAKSTNSAKVAAASLTDSENAAAAVKNQKAT"
     gene            complement(1965..2246)
                     /locus_tag="ACHKNB_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /pseudo
     CDS             complement(1965..2246)
                     /locus_tag="ACHKNB_RS20965"
                     /old_locus_tag="pgaptmp_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514848.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2299..3512
                     /locus_tag="ACHKNB_RS20970"
                     /old_locus_tag="pgaptmp_004194"
     CDS             join(2299..2590,2590..3512)
                     /locus_tag="ACHKNB_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4197597.1"
                     /translation="MTKNTRFSPEVRQQAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGGGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     KEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVS
                     RKAVAACNRVNRQFVAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWQVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAGVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNNDLAA"
     misc_feature    2580..2696
                     /locus_tag="ACHKNB_RS20970"
                     /old_locus_tag="pgaptmp_004194"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(3552..3911)
                     /locus_tag="ACHKNB_RS20975"
                     /old_locus_tag="pgaptmp_004195"
     CDS             complement(3552..3911)
                     /locus_tag="ACHKNB_RS20975"
                     /old_locus_tag="pgaptmp_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197598.1"
                     /translation="MKTQLKLELNMKKTLLARVVKGIAVCGLAMSSISYAATSVNAVH
                     NVVEESVWSIDYVDSGNTKAGQQDVSQPWGKLVVTLESGSPAIPSIHLPEGVRKGFDS
                     QFEWTHTESGVKYLGVI"
     gene            complement(4544..4927)
                     /gene="afaD"
                     /locus_tag="ACHKNB_RS20980"
                     /old_locus_tag="pgaptmp_004196"
     CDS             complement(4544..4927)
                     /gene="afaD"
                     /locus_tag="ACHKNB_RS20980"
                     /old_locus_tag="pgaptmp_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfaD family invasin"
                     /protein_id="HGU4197599.1"
                     /translation="MVSGGHAQAEELNIVPHKGMTGELRDGMKIATGRIICREAHTGF
                     HIRINARQQESRPGHYIIQGRRDSRHELRVRIAGEGWSPSVTGGQEGMVSYGQREQGV
                     FDVVADGSQRVEADEYIYSVTGECL"
     gene            4985..6197
                     /locus_tag="ACHKNB_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /pseudo
     CDS             4985..6197
                     /locus_tag="ACHKNB_RS20985"
                     /old_locus_tag="pgaptmp_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6205..6279)
                     /locus_tag="ACHKNB_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /pseudo
     CDS             complement(6205..6279)
                     /locus_tag="ACHKNB_RS20990"
                     /old_locus_tag="pgaptmp_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6374..6997)
                     /locus_tag="ACHKNB_RS20995"
                     /old_locus_tag="pgaptmp_004199"
     CDS             complement(6374..6997)
                     /locus_tag="ACHKNB_RS20995"
                     /old_locus_tag="pgaptmp_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4197600.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGLSQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRMVGNENTSSPYQQIAREVKVPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(7516..7743)
                     /locus_tag="ACHKNB_RS21000"
                     /old_locus_tag="pgaptmp_004200"
     CDS             complement(7516..7743)
                     /locus_tag="ACHKNB_RS21000"
                     /old_locus_tag="pgaptmp_004200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197601.1"
                     /translation="MARPAGSLNKRTISTNTTTPGEVKSFSLEHFLKKWRRTKMGRHT
                     WKVSPQTESQEQKQCAGAKDRELTGNSTAAE"
     gene            8067..8570
                     /locus_tag="ACHKNB_RS21005"
                     /old_locus_tag="pgaptmp_004201"
     CDS             8067..8570
                     /locus_tag="ACHKNB_RS21005"
                     /old_locus_tag="pgaptmp_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4197602.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTNTVHNEKPLRVRNQTPWR
                     HMTKRQRKNRKRINRLVEHWPELFNREKTQPLKVEIPDDLIQDIAIRELAFGAGALRA
                     AVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLMVLNDRQKPENLVD
                     YTTALIH"
     gene            complement(8564..9112)
                     /locus_tag="ACHKNB_RS21010"
                     /old_locus_tag="pgaptmp_004202"
     CDS             complement(8564..9112)
                     /locus_tag="ACHKNB_RS21010"
                     /old_locus_tag="pgaptmp_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197603.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHMASPASPLVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASVTVDEQCSQVVDLLQHSWQVISAGGQCRVERCFRVARGDTSGQYVALKTAALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            9570..>10020
                     /locus_tag="ACHKNB_RS21015"
                     /old_locus_tag="pgaptmp_004203"
     CDS             9570..>10020
                     /locus_tag="ACHKNB_RS21015"
                     /old_locus_tag="pgaptmp_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197604.1"
                     /translation="MKIYWTRKNIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFKVYDFLLPAQDTFPSDIIRSLCVVCPSILIWFQFSVYVMRKYYRHTLER
                     GKETETISERLIREADTREYELWRPAHRFFSIIFLIILLVCIHSLITT"
BASE COUNT         2718 a         2519 c         2196 g         2587 t
ORIGIN      
        1 ttaggttttg acctaaacac attcttcata agcatactga tttttagaac tttcaaataa
       61 acccagcgtt gaattcttgg aaaatacaaa aaaacaaggt tccgcgtgaa tgcaactaat
      121 atagacacgc caaagaaaaa tatacaaaaa acgcccaaaa ccagcccgat aagaactaaa
      181 aaaatctccg cacaatctct gacaaactga ataaaagaga aagaacgctc ataagtctga
      241 tgttttaata aagcatctga aataacattg gtaccaattg cacctgatat gaaaggaaga
      301 atataggtaa atattaagtg gtaacgctca aggcgcgata gtacatcacg cgatacatta
      361 ccagatgtag ccaattcttt gcaatgttct agaatttcta taaaagtaat gtgacctaga
      421 acaccaacac caataaagac gcccccacac ataaacacga tcgggcgtgt caacttaaag
      481 tcgatcagaa aagaaaatag acagaataaa agcataatta tgccaccagc aagagtaagc
      541 aagatagtcg gagagaacat aaaatcgaaa ttcatgggta tatatctaac tgtgatcgta
      601 ttcttttctt gattaaaaca ttgtgtgtcc taaagatgtt aatgtcaatg gagtataatc
      661 aatggcctat attcttgttg ttgacaaaaa ttcatctgat gaatgatcgc ctcaaaaaat
      721 acagagcctg tttaagattc tgtgtagatg tcttttctca gaagtgaccg tccgggcggt
      781 caccgaactc ggtgataaag ccagtccttc aacgacatcg tccatttcta gagcgaactt
      841 cagtttgaaa aagtgggcac ctacgttacc aatactggct taatagctac atacggctgt
      901 cagtttactc ttatgtatgt ttcttgcctg gactaaaaca acaaaatttg aacgactttc
      961 aataacagaa aagtcctgta catttgtccc aaccatttac gacgtataaa ccgtacactg
     1021 agttttagta agcaattcag tgcctttccc ggaggctaca gtatcgcctt taggcccctg
     1081 ttgacctgca tccctttttg cctcttgcgg ccccgtaaca cctttcggtc cctctgggct
     1141 ttccagaact taaataccct tcaccaaatt ttcagccttg gccgctgtgg attctgcagc
     1201 gttacatctt ttttagtctc cgtcgcctgc cacatctcct catattttga ggcttcagga
     1261 gatgatgcta atgacaccac ctgacaggct gactgtagcc tttgcgttta tttcgctgtt
     1321 cctgacgaat gcgtcactcg tttttacgtt gctttctgat ttttaactgc tgctgcggca
     1381 ttttctgagt ctgtcagtga ggcagccgcc actttggcgc tgtttgttga ctttgcatct
     1441 gtcgttgaca gcattttcgg atgtctttgc ctatgctgcg ctctgaaagg ctccgtcagc
     1501 gacaccgaca cctgatccac catcatatca aatcggagct gcgcttccgg agtaacatca
     1561 tcttcagtca tcgtccctac agggtccccg cctctaaaac cttgagcaaa tagaacaaca
     1621 cactactggt catactccac atccatgctg taacgtccag atttatcaga attttctgag
     1681 ccaccgagtt taccaccacc tcggtggtgt ggtacgtttt actttcagtc ctatctggct
     1741 ttttgtaccc cccatgccat ctttatgcac accagaaatt ttaactgtaa tacctttcca
     1801 ccaataaaaa agcccgcagc aataatgcca cgggctcagg gacagtagaa ctttacgctt
     1861 cctcaaacgc agttcaccgc atcaggcgaa tgaacatgtg tattatagca atatttaaag
     1921 gagataaagt gccatcttac gtcagaaacg gtattcgtgc aggtcgacaa cacattaaat
     1981 gtcagggaga tatgacagtg tcccgccggc atcgcaatca gcgatgagcc ctcctactga
     2041 catccattgc cgttttctga aaaatcccca cgttttcagc acctgaagat tacaggttta
     2101 ataccagtac cactgctgat tcgagttatt gaaattctgc tgtttcgtgg gacgaaacaa
     2161 cacaagggga atgataatct gccggcctcc ccctgaacat cataaacagt aacagttacc
     2221 gtgccactgg cataactgct attctgaacc gccccggaaa tcctggagac taaactccct
     2281 gagaaagagg taaacaggat gactaaaaat actcgttttt cccccgaagt ccgtcagcag
     2341 gcgattcgta tggttctgga aagtcaggat gaatatgact cacagtgggc ggcaatttgt
     2401 tccattgccc caaagattgg ctgtacgccg gagactctgc gtgtctgggt tcgccagcat
     2461 gagcgggata ccgggggcgg tgatggtggg ctcaccagcg ctgaacgtca gcgtctgaaa
     2521 gagctggaac gtgaaaatcg tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc
     2581 gcttattttg cgaaggcgga gttcgaccgc ctctggaaaa aatgatgcca ctgctggata
     2641 agctgcgtga gcagtacggg ggcggaccgg tatgcagcga actgcatatt gccccgtcaa
     2701 cgtattacca ttgtcagcaa cagcgacatc atccggataa acgcagtgcc cgtgcgcagc
     2761 acgatgactg gctgaagaaa gagatacagc gcgtatacga tgaaaatcac caggtatacg
     2821 gtgtgcgtaa agtctggcgt cagttgttac gggaaggtat cagagtggcc agatgcactg
     2881 tggcacgtct catggcagtt atgggacttg ccggtgttct ccggggtaaa aaggtccgca
     2941 ctaccgtcag ccggaaagcc gttgccgcat gcaaccgcgt aaaccgtcag ttcgtggcag
     3001 aacgtccaga ccagttgtgg gtggctgatt ttacctgggt aagcacatgg cagggcttcg
     3061 tttatgtggc gttcatcatt gatgtgttcg ccggatacat cgtgggatgg caggtctcat
     3121 catccatgga aacaacattc gtgctggatg cactggagca ggcgttgtgg gcccgtcggc
     3181 cgtccggcac aatccatcac agtgataaag gttctcagta tgtatcgctg gcctacacgc
     3241 agcggcttaa ggaagccgga ttactggcat caacagggag tactggcgac tcgtatgaca
     3301 acgcgatggc ggagagcatc aatggtcttt acaaagcggg ggtaatacac cgtaagagct
     3361 ggaaaaaccg gacagaagtg gaactggcca cactaacgtg ggtggactgg tataacaatc
     3421 gacgattgct ggaaaggctg ggccatattc ctccggcaga agcagaaaaa gcttattatg
     3481 cttccatcgg aaataatgat ctggcagcct gagttcacag ataaaatact ctccaggaaa
     3541 cccggggcgg ttcatattac tcctaagtat ttaacgcctg actcggtgtg ggtccactca
     3601 aactggctat caaaaccctt tctgacacct tctggcaggt gaatactggg gattgcaggt
     3661 gatccgctct caagtgttac aaccagcttt ccccaaggtt gggaaacatc ttgctgacca
     3721 gctttagtat taccgctatc aacatagtca atcgaccaga ctgactcctc tacaacgttg
     3781 tggactgcat taacactggt agctgcatag ctgatactac tcattgccag accacagact
     3841 gcaatacctt tgaccacacg agccagtaat gtttttttca tgtttaactc cagtttcagt
     3901 tgagttttca tcagatgtaa tccagccact ttccgttttc tctctctcag acatccagta
     3961 agccttctta ccctcaataa ccaccgttcc cggtgatgat ttcattcacg tccgccacaa
     4021 cggtggcccc gtgttcatcc ttcccgcttc cgggaaacac cattctgtct gaacctttcc
     4081 cggagaatgt cttgtccgga tatctcctga actttcccca gtaaaaatat tcactgcgtc
     4141 gtgattctgt ttcttctcat aagaaaaata aaaccgtgtc catataacgg acataataag
     4201 catctcccgt agatttaaat cagcatcagc ctgcttaaat tcatttccat ttatccggca
     4261 ccaggtttaa tatacactct ggtgacattc ccgtttccgg agtttatatt cttccggcaa
     4321 tgcaaaggat attataaata taatataact ctttcttcat ctgttatctg cgtgtgccac
     4381 tgacttttct ttaatgcaat atctgcaatt accttcaaca caaaccttca gtaccttgtg
     4441 ttttattctc ctgttctctg acctgttatt ctttatatat ctcattgcga tatgttgtta
     4501 ttaatactgt tattaatacc ctgtctgttc attcacgctc ccgtcacagg cattctcccg
     4561 tgacggagta aatatattca tccgcctcca cacgctggct gccatcagcc accacatcaa
     4621 acacgccctg ttccctctga ccataactga ccattccctc ctgaccacct gtcactgacg
     4681 gtgaccagcc ctctccggca atcctgacgc gcagttcatg gcggctgtcc cttctcccct
     4741 gtatgatata gtgccccgga cggctctcct gctgacgggc atttatccgg atatgaaaac
     4801 cggtatgcgc ctcacggcag ataatccggc cggtcgctat cttcattcca tccctcagct
     4861 cacctgtcat tcccttatgc ggaacaatat tcagctcttc tgcctgagcg tgaccaccgc
     4921 tgaccatcag taccactccg gttaatactg tagtggcaca gtaaatttgg ccacctgatt
     4981 aaaggtgata ttctcacctc aacacaaaac aggtgactta tgaacaagaa aaccaaacgt
     5041 actttcaccc ctgagttcag gctggaatgt gcacagctga ttgttgataa gggctattcg
     5101 tatcgacaag ccagtgaggc gatgaatgtc ggttctacca cgcttgagag ctgggtacgt
     5161 cagctcaggc gagagcgcca aggtattgcg ccctctgcaa cacccattac ccctgaacag
     5221 caacgtattc gcgagctgga aaagcaggtt cgccgactgg aggaacaaaa tacgatatta
     5281 aaaaaggcta ccgcgctctt gatgtccgac tcactgaacg gttttcgata gtttccagac
     5341 tgagtgacag ccactcagtt gtcagcctct gttccgcact gggaatacac cgcagcagtt
     5401 accggtactg gcgaaaacga cgcgatacgg ttaattcggc tcgcgtcagg ttgtgcagcg
     5461 aaatacgccg ggcatggaac cagagccggg gctctgcagg cgcgcgcacg ctggctgaaa
     5521 tgctgaccca aaacggcgtc ccgatgagcc gttatcgtgc cggacggctg atgaaatacc
     5581 tgaacctgag cagttgtcag cccggaaaac atcaatacaa aaatgcctgt caggagcata
     5641 cgtgtttgcc gaatctgctc gagcgtcagt tcgctgtacc ggagccagac agggtatggt
     5701 gcggagatat tacgtatatc tgggcaggaa atcgctggtg ctatctggcg gtcgtcatgg
     5761 atctttttgc ccgcagggtt atcggctgga gcctgtcagc gaatgccgat actgcactga
     5821 taagcagtgc cttgcggatg gcgtatgagg tgcgtggtca gccgcgggac gtcatgttcc
     5881 atagcgacca gggaagtcag tatacaggac tgaaatatca acagcttctc tggcgttaca
     5941 ggataaagca aagtgtcagc cgacggggaa actgctggga taacagtcct atggaacgct
     6001 tcttccgcag tctgaaaaca gaatgggtgc cgacggatgg ttacgtaggc aaggacgagg
     6061 cccggcaaca aattagcggt tatatcctga attattacaa tagcgtcagg cctcaccatt
     6121 acaacggtgg gttgacgccg gaagaatcag agaacagata ccgtttttac tgtaaaaccg
     6181 tggccaatat tacttgacca ctacacctct ctctgccagc cactcattca gttcatgtgc
     6241 cagagacaga ttgataaaat cgtaatcgga ccttgccatt cccgtttcct taacacatgt
     6301 cttcagactc agggataaag tataaaaatg tcgccctgca gttgccagag caatattccc
     6361 cctgatggtt gtatcagaga tggtatgccg gtactttgcg ggttgttcct gtcaggttat
     6421 cgtgcacctc cacggtcgtg gtcaccaccg gcattccggc ctttctcagc ctcagaacat
     6481 cagccgcaat acgctgactg ccagaccaga acaggccgtc cagtgcagtc accagtaaac
     6541 ccgcctccag tgcatacttc agccgttcac ggggcacttt cacttcccgg gcaatctgct
     6601 gatatggcga tgatgtattt tcattcccaa ccatccggcg aatacgatga gacagatgat
     6661 accggaatgt atccggtaca ccagaaagac tggccttcag gctgtacacg cagccaaacc
     6721 gtttatcgtt gaacaccaca tttttctggc tgaggcccca ttcttcccga agcgcggcaa
     6781 tcagttgcgg cgtgcgggtt aaaagcagcc gaaaaggcag ttcaaaactg gtgacgtaat
     6841 ccacattcag cagggcaata cgcagccgtt cttctggtcc ggcctctgtc tgccggcact
     6901 cctccagaac atcctgccac tgctggcgaa gacggggcga ttcgttccgt tctgtaaaac
     6961 agtatttatc cgccagataa tcaattcgtg tacgcatact gcaaagaatt ccgtataaaa
     7021 attcatctga caaaacttta tcagtccata aaaatcaacg gaagcacaga tgattcactc
     7081 gtccattacc aacggatttt gccaataacc ctggcgttga caacgggtaa cataaaagac
     7141 aaagactgaa caactgtttt taacggcgat actgcatgat ttcatcgcca tccgtcaccg
     7201 tcatatccac cagatgaaac aactctgtgc aggttttatc cgcaagttcc ggattatcaa
     7261 aacacgtcgg tcagttcatg ttgtacgggg acaggctgcg ctccccatga cagaacatta
     7321 tcgatatcac cagcatacgt gccactcaaa gtgttgcggc atcgtacggc gtggtcgtgt
     7381 tcttctcttt ttcctgttca caataaccag cttaacggtc agaaggcatt ttcacccgga
     7441 ttatattcac ctgcacaacc atgttcagcc atgaccgctg ataagtgaac atccggagta
     7501 tacatggcat aaagattatt ctgccgctgt tgaattgccg gtaagctccc ggtcctttgc
     7561 tccggcacac tgtttttgtt cctggctctc tgtctgaggt gaaaccttcc aggtatgtcg
     7621 ccccattttt gttcgtctcc atttttttaa gaagtgctca agtgaaaaac tttttacctc
     7681 tccgggagtt gtcgtatttg ttgatattgt tcgtttgttt aaagaacctg ctggtcttgc
     7741 cattaatatt acctgctgat tgcttcacgc cgggctcctc gcactgtgcg cgggttattg
     7801 tttcgttttc agtcccgggg gcgccaggac tctgtttgca gaccataacg gcctgacctg
     7861 tactgtttct tcggctcttt gatgcccgtt tacgccattt gttccctgcc cctctgtctg
     7921 ataacgaata cgctcactac gcacagcact gaacaatacc ggcaacagca tgtcttacat
     7981 gacattcgct gtgccatcca ccggaaaaca ttttcagata ccggatgagg taccactgcc
     8041 attgacagca cgaggtaaca atactgatgg taacgctgac cattaaccgt aaaccgcagg
     8101 gtattttcag taagtcaccg gcaacgccgc tgcaggataa aacgaacaca gtacacaacg
     8161 agaagcctct tcgggtgagg aatcaaacac catggcgaca tatgacaaaa cgccagcgca
     8221 aaaaccgtaa gcgtattaat cgtcttgtgg aacactggcc tgagttattc aaccgggaaa
     8281 aaacccaacc gcttaaagtt gagatacctg acgacctgat acaggatatc gccatcagag
     8341 aactggcatt tggcgcaggt gcattacgcg cggcagtagc gtcttatgtg cagtctccgc
     8401 gctattaccg tgcgttaatg gctggcggcg cccgttacga cctgaagggg cagccgtgcg
     8461 gggaagtgac tccacaggaa cagaaagagg cagagacccg gctaatggtg ctgaatgacc
     8521 gacaaaagcc agaaaacctc gttgactaca caactgcgct gattcactga gctgtaatca
     8581 atgtacagcg ctgtaccgga cgatgggtaa tggcggtcac aaccggtaac cccagagaca
     8641 atgcggcagt ttttaacgct acatactgac cggatgtatc accccgggca acccgaaaac
     8701 acctttccac ccggcattgt ccgccagcac ttatcacctg ccagctatgt tgcagtaaat
     8761 ccaccacttg cgaacattgt tcatcaactg tgacactggc tcgcgggtga caaatactcg
     8821 tctggctgag cagcaacagg catcgaattg ttgctcctcc atgttgttcc cgcaaccagc
     8881 gtaataccag cggcgatgcc ggactggcca tgtgattacg ctcagtaata cgttcgtgca
     8941 cctgtcggta aaaggaagta cagaatgcat gaatctgttg cccgttgata tttccttctg
     9001 ccgaatggag tgtgaagtaa aatgccagca aatgcggcca gtggtccacc gcctgctgaa
     9061 caaaacgccg gatgtctccc ggctctgaaa ttaaggcttc ggttatttgc acgaaatgtt
     9121 ccttttcaaa ccctgttgga ttttgtcaag ccggtacaga cacagtaaag cgcgcatcaa
     9181 cacctgaaag gtacgataca aacacaagtt acaaacaata agaacggaag gacaattaat
     9241 cagtgaggaa taataactaa attaaccaaa agaatgttac tgacaatacc accaatactt
     9301 aaatagcaat atattaatta atgtaagaac agatgcccag taagacatat acacaataca
     9361 tccacaaaca cactcttttc attaaataaa tcacctgatt aaaccatgaa agtgtgatgt
     9421 aagtcaaaat tagtgacaca atcaattgat aatgattatc tctatcatta tcgtgaaatt
     9481 cgagaatgaa aggagaagta gtcattataa gacttttata aatcgtcatc atcatagtaa
     9541 agtagaacac tttttatatg agttactata tgaaaatata ctggacaaga aaaaacattc
     9601 ctgaactttc agcacttcca ccttcattaa gaaagaaaaa ttttactgac gcatataatg
     9661 ctgcatcttc ccatatcgaa tactggatag gtgctggcgt ctcatttatc tcgatgatga
     9721 ttttattcaa agtatatgac tttcttttac ccgcccaaga tactttcccg agtgatatca
     9781 taagaagttt atgtgttgtg tgtccatcca tattaatctg gtttcagttt tcggtatatg
     9841 tcatgaggaa atattacaga catactctgg aacgaggtaa ggaaaccgaa accatttcag
     9901 aaaggttgat acgtgaggct gatacacgag aatatgaact atggcgtccg gcccatcgct
     9961 ttttctccat tatttttctc attattctat tagtttgcat tcattcactg ataacaacta
//
LOCUS       DAZFVO020000172         9995 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFVO020000172 DAZFVO020000000
VERSION     DAZFVO020000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.173"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..293
                     /locus_tag="ACHKNB_RS21020"
                     /old_locus_tag="pgaptmp_004204"
     CDS             <1..293
                     /locus_tag="ACHKNB_RS21020"
                     /old_locus_tag="pgaptmp_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4197605.1"
                     /translation="FFEDIRNAIKKRKKIKINYCDKKDSYTSRVIWPIGLVYMESCWL
                     LVAWCEMRNDFRHFRTDKIEDIVQLDSNYSESRTILLKKWRIKEGICEGKEY"
     gene            365..1111
                     /locus_tag="ACHKNB_RS21025"
                     /old_locus_tag="pgaptmp_004205"
     CDS             365..1111
                     /locus_tag="ACHKNB_RS21025"
                     /old_locus_tag="pgaptmp_004205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGU4197606.1"
                     /translation="MNFQNKIAVITGSTTGIGEAVAEQLHKHGAKVVIVSRSSEQAKQ
                     KAEQLSSQGQQAMGIGCDVSQPEQVRQMIDEVIKHFGRLDYAVNNAGLTGEHGKNITE
                     QTVENWDKVIATSLSGVFHCLKYEIPQMMKFGGSIVNLSAVNGLVGIPGLAPYTVAKH
                     GIIGLTQTAALEFACQGIRINAVAPGYVQTQRMSEFPENIVSSFANSHPMKRMAKMQE
                     IADFILFLLSDSSTFCTGGVYPIDGGYLAE"
     gene            complement(1414..1815)
                     /locus_tag="ACHKNB_RS21030"
                     /old_locus_tag="pgaptmp_004206"
     CDS             complement(1414..1815)
                     /locus_tag="ACHKNB_RS21030"
                     /old_locus_tag="pgaptmp_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HGU4197607.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(1856..2821)
                     /locus_tag="ACHKNB_RS21035"
                     /old_locus_tag="pgaptmp_004207"
     CDS             complement(1856..2821)
                     /locus_tag="ACHKNB_RS21035"
                     /old_locus_tag="pgaptmp_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197608.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNPDAVVFSVKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPEQRCVNHISEPDTEIPPGFRG"
     gene            complement(2802..3323)
                     /locus_tag="ACHKNB_RS21040"
                     /old_locus_tag="pgaptmp_004208"
     CDS             complement(2802..3323)
                     /locus_tag="ACHKNB_RS21040"
                     /old_locus_tag="pgaptmp_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HGU4197609.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERSNID
                     VQRVTYEGGAVDQRALSVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            complement(3307..3537)
                     /gene="dicC"
                     /locus_tag="ACHKNB_RS21045"
                     /old_locus_tag="pgaptmp_004209"
     CDS             complement(3307..3537)
                     /gene="dicC"
                     /locus_tag="ACHKNB_RS21045"
                     /old_locus_tag="pgaptmp_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HGU4197610.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGDLVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3621..4028
                     /gene="dicA"
                     /locus_tag="ACHKNB_RS21050"
                     /old_locus_tag="pgaptmp_004210"
     CDS             3621..4028
                     /gene="dicA"
                     /locus_tag="ACHKNB_RS21050"
                     /old_locus_tag="pgaptmp_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional dual regulator DicA"
                     /protein_id="HGU4197611.1"
                     /translation="MDTKNLTIGERIRYRRKNLKHTQRSLAKALKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPTPPVEKPVALAPKELELLELFNALPES
                     EQDTQLAEMRARVKNFNKLFEELLKARQRTNKR"
     gene            4195..4350
                     /gene="ydfA"
                     /locus_tag="ACHKNB_RS21055"
                     /old_locus_tag="pgaptmp_004211"
     CDS             4195..4350
                     /gene="ydfA"
                     /locus_tag="ACHKNB_RS21055"
                     /old_locus_tag="pgaptmp_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HGU4197612.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4352..4453
                     /gene="ydfB"
                     /locus_tag="ACHKNB_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /pseudo
     CDS             4352..4453
                     /gene="ydfB"
                     /locus_tag="ACHKNB_RS21060"
                     /old_locus_tag="pgaptmp_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            5414..5602
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /gene_synonym="ftsT"
     CDS             5414..5602
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS21065"
                     /old_locus_tag="pgaptmp_004213"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4197613.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5599..5790
                     /gene="ydfD"
                     /locus_tag="ACHKNB_RS21070"
                     /old_locus_tag="pgaptmp_004214"
     CDS             5599..5790
                     /gene="ydfD"
                     /locus_tag="ACHKNB_RS21070"
                     /old_locus_tag="pgaptmp_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HGU4197614.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            5884..8361
                     /locus_tag="ACHKNB_RS21075"
                     /old_locus_tag="pgaptmp_004215"
     CDS             5884..8361
                     /locus_tag="ACHKNB_RS21075"
                     /old_locus_tag="pgaptmp_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4197615.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVAVATAIEAGDERRARAKFHW
                     QFLEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESD
                     PMNVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMP
                     EVNAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLA
                     RAKANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHR
                     WAKKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYIC
                     EYLTTKGVFENEETDPGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPF
                     YFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDS
                     EQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRD
                     SVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPD
                     CGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETAD
                     RSIKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQV
                     WGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERH
                     NALDDARYQAKYVSVIWQKLIPSQADS"
     gene            8434..8685
                     /locus_tag="ACHKNB_RS21080"
                     /old_locus_tag="pgaptmp_004216"
     CDS             8434..8685
                     /locus_tag="ACHKNB_RS21080"
                     /old_locus_tag="pgaptmp_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4197616.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8720..>9995
                     /locus_tag="ACHKNB_RS21085"
                     /old_locus_tag="pgaptmp_004217"
     CDS             8720..>9995
                     /locus_tag="ACHKNB_RS21085"
                     /old_locus_tag="pgaptmp_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HGU4197617.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEARKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMK"
BASE COUNT         2991 a         2262 c         2247 g         2495 t
ORIGIN      
        1 ttttttttga agacattcgc aatgctataa aaaaaagaaa gaaaatcaaa ataaattatt
       61 gtgataaaaa agattcttac acttcacgag tcatatggcc catcggattg gtatacatgg
      121 aatcgtgctg gcttttggtt gcatggtgcg agatgagaaa tgattttcgt cattttagaa
      181 ctgacaaaat tgaagatatt gtgcaacttg actccaacta tagtgaaagc aggacaattt
      241 tattgaaaaa atggagaata aaagaaggga tctgcgaggg gaaagagtac tgacaaaaac
      301 tgtcacagat ggtttgtata ctatcccaga tgaaagactc aattctactt catagggggt
      361 ggttatgaat ttccagaata aaattgccgt tataacagga agcactacag gtataggtga
      421 ggccgtcgca gagcaattac ataagcatgg tgcaaaagtt gttattgtat cccgctcgtc
      481 agaacaggct aaacaaaaag cggaacaatt atcttcacag ggacaacaag ccatggggat
      541 cggatgtgat gtgtcacagc ccgaacaagt acggcaaatg atagatgagg ttatcaaaca
      601 tttcggcaga cttgattatg ctgtaaataa tgcaggctta acaggtgaac atggtaaaaa
      661 tataacagag cagacagttg aaaactggga taaggttatt gctacctcat tgagcggcgt
      721 ttttcactgc ctaaaatatg aaatacccca gatgatgaag tttggtggat ctatagttaa
      781 tttatctgca gtaaatggtc tggttggcat tcctggatta gcaccttaca ccgtggctaa
      841 acatggtata atcggtttaa cccaaaccgc agcgctagag tttgcatgtc aaggcattcg
      901 aatcaatgca gtcgctccgg gctatgtcca aacccaaaga atgagtgagt tccctgaaaa
      961 tattgtaagt agtttcgcaa atagccaccc tatgaaaagg atggcaaaaa tgcaggaaat
     1021 agcagacttc atattgttct tgctgtcaga cagttctaca ttctgtaccg gaggcgttta
     1081 tccaatcgat ggcggttatt tagctgaata aaaaagcaca ttattttgaa gtgtaagaat
     1141 tgctttgcca aagtggtagg ctcaatggaa gcgttagaat ataaaattaa cgcttccatt
     1201 acagggctag tgaaaacaat attttcgtta gggatctata tgtgtataga tgatcggttc
     1261 tccattggtt catacatcat gccattagca acgtccgctc ctggcacaaa gcggacaacc
     1321 acgccagatc taccctgtgc catgaaaaat gagaatgtca attcactcct gaactaatgc
     1381 tttttaatct agtaacgtct aaaataccta acattatccc tgataaaatg ccagtatgcg
     1441 ctgcataact tcactcatcc ggcactcgcg acagattatg tttaggcgac tgtcgtagcg
     1501 acgtatttct ccatcaggta acgaccagat aaggtccgga tcaaccacag ctgttttctt
     1561 cacctttgcc ctcgatagtt ttttgcgggc gttttgccag tccttacgcg cctgttcaga
     1621 cgggaataac ccgtagccgg agttgtatac atcgccactg gctaccagct ctctggcaag
     1681 aacgctcatc agatatctag tcgcacctgt tttagcttcc agttgccgta acgtctcacg
     1741 accactctgg cgcacgagtt ccaccacctg ccctttaatt ttttcccgct cttcttgtgt
     1801 aaaaactttt gccacaagtc ccccttaaaa ttacctcatg acctgaaatc aaaacttatc
     1861 ctctgaaacc aggcggaatt tctgtatccg gttcagaaat atgattcaca caacgctgtt
     1921 caggtgaacg tcccaggcgg atgaccagtt cgtcccattt ttcgcggagt tttgacgggc
     1981 tcatgatgtt ttttacccag aatggatccc gctgtacccg accaaacatt tcgcaaattt
     2041 gtctatggct tctgccatcc agcatccgca ttgtgcgcac gtcattggcc caggctgtcc
     2101 agttaggctc ttttggtcgc atgatctctc catcgtcgct ggcagcctgt tcgtaaaggt
     2161 tcacgatccg cccccaaatc cactgcgcac acgccaaatc ctccctgcta ccccactggc
     2221 gttttttcac actaaacaca accgcgtcgg ggttccgggt taaaaaatcc tgttcagtcg
     2281 tctgcgggtc cggttgcgaa gcttccggac gaaaagtgtt tttattctct gtagtaatct
     2341 ctgttgtatt ctctgtaaga tcatcaggcc attttgaccc gatgacattg ggtcgttttg
     2401 aaccaatgga gcgtttcatt ttgacctctt ccatcgtgtc attttgacct gatggagcgg
     2461 cgcattttga accgatggat tcgttcaatt tgccaccatc taaaagctcg ctcccatagt
     2521 tgatcgtgta gaaattggtc atatcgcgct ttgatttatt gagcttttca caacgcaaaa
     2581 gccccagcgt tttcagactt gcaaacgcgc gctttaacgt tgactctgac cagaatggga
     2641 actgttccag ccattgttcc gttgtgttat aaatccagcg aacaccatca cattccatac
     2701 cggagttggt atctctcaac cagtagtgca gttgttgcaa aacaatggct tcgtttaagc
     2761 cgattttcat tgccagctgc gtgtttataa ccagtgggcg ttcagcaaaa agaagactca
     2821 taattccatc cagctttttg ttggtattgc tgtcgatacg caagcttgaa agcaattgct
     2881 ttttctataa gttcgtcagt ttcacgatct acaacggcag gatctgcaaa aagcagtccg
     2941 gactccacca catcgccata ttctttattt aacccagcga tcatgtacgt aatgctcttt
     3001 ccgtcactaa tttcacgata caacctgaaa tcactaatcc ggatagcctc cataattgca
     3061 ggcactagcg ctgtgaactt ttcacgctta tccctggtgt cgatagcctt ccagcgttca
     3121 aatatcttca ctcgattaac gctaagcgct cgctgatcaa ccgcgccacc ttcatatgtg
     3181 acacgctgaa catcgatgtt cgagcgctct ttcaaagccc agaatgcttc agtgattaat
     3241 atcgtcgcct gctcctgtgt cattcctggt cgacatatcc aggcatccag agcctcacga
     3301 gcctgttcag gagtgatttt cattgttcaa ccgccccgcc cgcttcgtct tacgatattc
     3361 gtcataaact ttgggatcat actgaagctc cccgccagat gcctcctgta gacgcatcgc
     3421 gcgaccttcg ggaactaaat cccctttcca gctataaagc gaagccaaac gaatacctgc
     3481 tgcttgtgca agttttgttt ttgaaccgaa atacaaaaga gcgtcagttt taagcattta
     3541 aaacacctta gatgttagtc atgactaaca aaatagatgt taacaaaaac atagtcaata
     3601 cgatttagca ttagctaact atggatacaa aaaatttaac catcggcgaa cgcattaggt
     3661 atcgtcggaa aaacctcaaa cacacccaaa ggtctcttgc taaagccctg aaaatctccc
     3721 atgtgtctgt atcacaatgg gaacgggatg atagtgaacc tacagggaag aacctttttg
     3781 ccctcagcaa agtattgcaa tgctcaccaa catggattct atttggcgat gaagacaagc
     3841 aaccaacacc acctgttgag aagccggttg ccttagcccc caaagaacta gagctccttg
     3901 agctgtttaa tgcactgcca gaatcagaac aggataccca gctcgccgaa atgcgagctc
     3961 gagtaaaaaa tttcaataaa ctctttgaag aattactaaa agcccgtcag cggacaaata
     4021 aaagataaca tcatcaatga gttatctttt attatatcaa ttatgttagc cataacatac
     4081 aaaatcactt gaccaatatg ttagtcatgg ctaatcttgt ttacatcaac acaccgcacg
     4141 gtgttctcag caaacagttc cgccaccccg gcgttaaggg gaaatgaggt cagcatggat
     4201 actatcgatc ttggcaacag cgaatctctg gtatgtggcg tgttccccaa ccaggacggc
     4261 acgttcaccg cgatgacgta taccaaaagc aaaacgttta aaaccgaaaa tggtgcccgt
     4321 cgctggctgg aaagaaactc aggtgagtga tatggatttc gacacaatca tggaaaaggc
     4381 ttacgaagaa tacttcgaag gccttgccga aggcgaagaa gctctcagct tcagtgagtt
     4441 taaacaggca ctcagaataa gaatgtgctc tcacaatgac gcggagcaca aatatgagaa
     4501 gcaaaatcag accgcagaaa attttgttct ggaacccgga gaaacgcttt tcaaaattcc
     4561 cgttacgtgc cccatttgcg gttttacatc agaagaactt gacgactcct gtaacaatca
     4621 ggaaacaacc aagtatgtcg aagatgatac cgagtgcgca cgaagaacga ttatatccac
     4681 gagtccaaac tccaggacca ataaatctca ccttgagagg gtgattaatc cactccccca
     4741 aaccaataaa aaagatgccg gaggacaaaa aacccaatgg aacaacggga tatctgtcaa
     4801 aaaaagacgt tccattaaag acaaacaacg cagcgccaat aactgtaagc gctttatacc
     4861 agtaatcaat tttcatgttc ttaagcggat ttattggtgg ttgcgacatt gcttaatgaa
     4921 tccttaaaac tgtggtgatt ttaaggatac cacctcgcct gacgtggtta aaagcaggca
     4981 cacaacacga aagcgcacgg cgaagttcgt ctcactgtac ggtgtcgtta aatttaattc
     5041 gaccgtgcgc ttccggttgt ggcaacccgc gaaatggcgc ggcggtaagt atggcggggt
     5101 tattccttcc ctgttgagga caccgagttg tcaggttgac catacgctta agtgacaacc
     5161 ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcagtatc agttttactc
     5221 cgtgactgct ctaccgccgt ttttaaagtg aattttgtga tgcggtgaat gcggctaagc
     5281 gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gattctctgt atccggcgtt
     5341 aattgttaac tggttaacgt cacctggagg caccaggcac tgcatcacaa aattcattgt
     5401 tgaggacgcg ataatgaaaa cgttattacc aaacgttaat acgtctgaag gttgttttga
     5461 aattggtgtc actatcagta accctgtatt tactgaagat gccattaaca agagaaaaca
     5521 agaacgggag ctattaaata aaatatgcat tgtttcaatg ctggctcgtt tacgtctgat
     5581 gccaaaagga tgtgcacaat gaattcagca tttgcgcttg ttctgacagt ttttcttgtt
     5641 tccggagagc cagttgatat tgcagtcagt gttcacagga caatgcagga gtgtatgact
     5701 gcagcaaccg aacagaaaat tcccggtaac tgttacccgg tcgataaagt tattcaccag
     5761 gataatatcg aaatcccggc aggtctttaa aacagttccg taataaatat ccggtttcat
     5821 tcttatatgc cagcaatggc agggatttgt tcatccttaa atctgtcatg aggttaaaac
     5881 aaaatgagta aagtctttat ttgcgccgct attcctgacg aactggcaac aagggaagaa
     5941 ggcgctgtgg ctgtggctgt agccacagcc attgaagctg gcgacgaacg ccgtgctcga
     6001 gcaaaatttc actggcagtt cctggaacat tatccggctg ctcaggactg cgcttataaa
     6061 tttattgtct gcgaggataa acctggcata ccccgccctg ccctcgattc atgggatgct
     6121 gaatatatgc aggaaaaccg ctgggatgag gagtctgctt cttttgtccc ggttgagact
     6181 gaatccgatc cgatgaacgt cacttttgac aagctggccc ctgaagtaca gaacgctgtc
     6241 atggttaagt tcgacacatg tgaaaacatc accgttgata tggttattag cgcacaggaa
     6301 ttgttgcagg aagacatggc aacattcgac ggacatatcg ttgaagcgtt gatgaaaatg
     6361 ccagaagtta acgccatgta tccggagctt aagttgcacg ccattgggtg ggttaagcat
     6421 aaatgtattc ctggtgctaa atggcccgaa attcaggcag agatgcgcat ctggaaaaaa
     6481 cgtcgcgaag gtgaacgcaa ggaaaccgga aaatacacgt ctgttgttga tctcgcccgc
     6541 gccaaagcca atcaacagta cactgaaaat tcaacaggaa aaatcagccc ggtcattgct
     6601 gccattcatc gcgaatacaa gcagacatgg aaaacactgg atgacgaact ggcctacgct
     6661 ctctggcctg gtgatgtgga tgccggaaac attgacggca gcatccatcg ctgggcaaaa
     6721 aaagaagtta tcgacaacga ccgcgaagac tggaagcgta tctcggcatc aatgcgcaaa
     6781 cagcctgatg cccttcgcta cgaccgccaa actatttttg gccttgtccg tgagcgtccg
     6841 atcgacattc acaaagatcc cgtagcactg aacaaatata tctgcgaata cctgacgaca
     6901 aagggcgtgt ttgagaatga agaaacagac ccgggcactg ttgatgttct ccagtcatca
     6961 gaaacacaaa ctgatgcagt ggaaaccgag gtatctgata tcccaaaaaa tgaaaccgcg
     7021 ccggaagctg aaccatctgt agagcgtgag gggccgttct atttcctctt cgcagataag
     7081 gacggagaaa aatacggtcg cgcaaacaaa ctttctggtc tggataaggc actggctgct
     7141 ggcgccactg aaatcacaaa agaagaatat tttgcccgaa aaaatggcac atacacgggc
     7201 ttaccgcaaa atgtagatac cgctgaagat tcagaacaac cagagccgat aaaagttacc
     7261 gctgacgaag taaacaaaat tatgcaggca gccaatatca gccagcctga cgccgataag
     7321 ttgcttgctg catcacgtgg tgaatttgtt gaagggatta gtgacccgaa tgatccgaaa
     7381 tgggttaagg ggatccagac ccgcgattct gtgaaccaga accagcatga atcggaacgg
     7441 aactaccaaa aagcggaaca aaacagccca aatgcgttac aaaacgagcc agaaacgaaa
     7501 cagcctgaac cagtggcgca acaggaagtg gaaaaagtct gcaccgcctg cggtcagacc
     7561 ggcggcggca actgccctga ttgtggcgcg gtgatgggcg acgcaacata ccaggaaaca
     7621 ttcgatgaag agtatcaggt tgaagttcag gaagatgatc cggaggaaat ggaaggcgct
     7681 gaacatccac acaaggagaa cactggcggc aatcagcatc acgatagcga taatgaaact
     7741 ggcgagacgg cagatcgctc aattaaggtg aacggtcatc acgaaatcac atccaccagc
     7801 aggacgtgtg accatctaat gatcgacctt gaaaccatgg gaaaaaatcc tgatgccccg
     7861 atcatctcaa taggtgcaat atttttcgat ccgcaaaccg gagatatggg accggaattt
     7921 agtaagacta tcgatctgga aactgctggc ggagtcattg atcgggacac cattaaatgg
     7981 tggcttaagc aatcacgcga agcgcaatct gccattatga ccgatgaaat cccgttagat
     8041 gatgcactgt tacaattgcg ggaatttatc gacgaaaact ccggtgaatt ttttgttcag
     8101 gtctggggaa atggagccaa cttcgacaac acgattttgc gccgttcata cgagcggcag
     8161 gggatcccct gcccgtggcg ttactacaac gatcgcgatg tacgcacaat cgttgagctg
     8221 gggaaagcca tagacttcga tgccagaacg gctattccat tcgaaggtga gcgccataat
     8281 gcacttgatg acgcccgtta ccaggcaaaa tacgtttcag ttatctggca aaaactgatc
     8341 ccgagtcagg ctgattctta atgttcaact gtcgccggtt gtgactggta ttctgcaacc
     8401 ggcgctcgtc tgatgtaaga gataaagaaa tcgatgagcg aagtaatcat gattgtctct
     8461 cccggcaaat gggtatccga agagcagtta attgcgctga aaggaataaa aaaaggtacg
     8521 ttaaaaaagg cccgggaaaa atcgtttatg gaaggaaggg aatataagca tgtcgctcat
     8581 gacggtatgc catgggataa cagtccatgc ttttacaacc tggaagaaat tgatcgctgg
     8641 attgagcgcc aggcatcagc gagaccaaga cgtcatctta cttgactaaa agccacacta
     8701 actaatgaga gaagttgaaa tgaaatatcc gacaggcgtg gaaaaccatg gagggaaatt
     8761 acgtatctgg tttgtttata aaggcgtaag agtcagggaa aatctggggg ttcctgacac
     8821 agcaaaaaac aggcgcgttg caggtgaact acgctcctct gtttgttacg caataaaaac
     8881 tggtgttttc gactatgcaa aacagtttcc ctcctcacgc aatctggaaa aatttggtga
     8941 ggcccgacaa gatttaacca taaaagaact ggctgaaaaa tttctggcac tgaaagaaac
     9001 tgaagtcgcc aaaacatcac tcaacacata ccgtgccgtc atcaaaaata tcctgagcat
     9061 aatcggtgaa aaaaatcttg cctcatcgat taataaagaa aaattactgg aggctcgtaa
     9121 agagttactg actggatacc agatccccaa aagtaactat attgttacac aaccagggag
     9181 atcggctgta actgtaaata attacatgac aaatcttaac gccgtgttcc agtttggtgt
     9241 tgataacggt tacctggcag ataatccgtt taaggggatc tcgccattaa aggaatcaag
     9301 aaccattccg gatcctcttt cgcgggaaga atttatccgt cttatcgatg cgtgcagaaa
     9361 tcagcaagca aaaaatttat ggtgtgtttc tgtttatact ggcgttcgcc ctggtgagct
     9421 gtgtgcactt ggatgggagg acatagatct gaaaaatgga acaatgatga tcaggagaaa
     9481 tttagcaaaa gaccgtttca cggtaccaaa aacacaggcg ggaaccaatc gggtcattca
     9541 tcttattaag ccagcaatcg acgctctccg gagtcagatg acattaacga gactgagcaa
     9601 agagcatatc attgatgttc acctcagaga gtatggcaga acagaaaaac aaaaatgcac
     9661 ctttgttttt caacctgaag tgtcagcgag agtaaaaaat tatggtgacc attttaccgt
     9721 tgactcaata aggcagatgt gggacgcagc gataaaacgt gccggactcc gccatcgaaa
     9781 atcatatcag tcgagacata cttatgcctg ctggtcgctg acagctggtg ctaacccggc
     9841 atttatagca aaccagatgg gccatgcaga tgcgcaaatg gtatttcagg tatacggaaa
     9901 atggatgtct gaaaacaata atgcacaggt agctttgtta aatacacagt taagcgagtt
     9961 tgccccaacc atgccccata acgaagcaat gaaaa
//
LOCUS       DAZFVO020000173         9917 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFVO020000173 DAZFVO020000000
VERSION     DAZFVO020000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.174"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1182
                     /gene="ybaT"
                     /locus_tag="ACHKNB_RS21090"
                     /old_locus_tag="pgaptmp_004218"
     CDS             <1..1182
                     /gene="ybaT"
                     /locus_tag="ACHKNB_RS21090"
                     /old_locus_tag="pgaptmp_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGU4197618.1"
                     /translation="AALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFF
                     RRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGII
                     AVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFS
                     CIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSAL
                     ELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSEREL
                     PKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLR
                     HDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKT
                     V"
     gene            1213..1310
                     /locus_tag="ACHKNB_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /pseudo
     CDS             1213..1310
                     /locus_tag="ACHKNB_RS21095"
                     /old_locus_tag="pgaptmp_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1307..1714
                     /gene="cueR"
                     /locus_tag="ACHKNB_RS21100"
                     /old_locus_tag="pgaptmp_004220"
     CDS             1307..1714
                     /gene="cueR"
                     /locus_tag="ACHKNB_RS21100"
                     /old_locus_tag="pgaptmp_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HGU4197619.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1715..2173)
                     /gene="ybbJ"
                     /locus_tag="ACHKNB_RS21105"
                     /old_locus_tag="pgaptmp_004221"
     CDS             complement(1715..2173)
                     /gene="ybbJ"
                     /locus_tag="ACHKNB_RS21105"
                     /old_locus_tag="pgaptmp_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HGU4197620.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2170..3087)
                     /gene="qmcA"
                     /locus_tag="ACHKNB_RS21110"
                     /old_locus_tag="pgaptmp_004222"
     CDS             complement(2170..3087)
                     /gene="qmcA"
                     /locus_tag="ACHKNB_RS21110"
                     /old_locus_tag="pgaptmp_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGU4197621.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3233..3910
                     /gene="fetA"
                     /locus_tag="ACHKNB_RS21115"
                     /old_locus_tag="pgaptmp_004223"
     CDS             3233..3910
                     /gene="fetA"
                     /locus_tag="ACHKNB_RS21115"
                     /old_locus_tag="pgaptmp_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HGU4197622.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3897..4676
                     /gene="fetB"
                     /locus_tag="ACHKNB_RS21120"
                     /old_locus_tag="pgaptmp_004224"
     CDS             3897..4676
                     /gene="fetB"
                     /locus_tag="ACHKNB_RS21120"
                     /old_locus_tag="pgaptmp_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HGU4197623.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4739..5593)
                     /gene="cnoX"
                     /locus_tag="ACHKNB_RS21125"
                     /old_locus_tag="pgaptmp_004225"
     CDS             complement(4739..5593)
                     /gene="cnoX"
                     /locus_tag="ACHKNB_RS21125"
                     /old_locus_tag="pgaptmp_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HGU4197624.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5654..6463)
                     /gene="ybbO"
                     /locus_tag="ACHKNB_RS21130"
                     /old_locus_tag="pgaptmp_004226"
     CDS             complement(5654..6463)
                     /gene="ybbO"
                     /locus_tag="ACHKNB_RS21130"
                     /old_locus_tag="pgaptmp_004226"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HGU4197625.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6453..7076)
                     /gene="tesA"
                     /locus_tag="ACHKNB_RS21135"
                     /old_locus_tag="pgaptmp_004227"
     CDS             complement(6453..7076)
                     /gene="tesA"
                     /locus_tag="ACHKNB_RS21135"
                     /old_locus_tag="pgaptmp_004227"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HGU4197626.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            7047..7733
                     /gene="ybbA"
                     /locus_tag="ACHKNB_RS21140"
                     /old_locus_tag="pgaptmp_004228"
     CDS             7047..7733
                     /gene="ybbA"
                     /locus_tag="ACHKNB_RS21140"
                     /old_locus_tag="pgaptmp_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HGU4197627.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7730..>9917
                     /gene="ybbP"
                     /locus_tag="ACHKNB_RS21145"
                     /old_locus_tag="pgaptmp_004229"
     CDS             7730..>9917
                     /gene="ybbP"
                     /locus_tag="ACHKNB_RS21145"
                     /old_locus_tag="pgaptmp_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HGU4197628.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLN
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKQIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLW"
BASE COUNT         2190 a         2580 c         2752 g         2395 t
ORIGIN      
        1 gctgcattgc taatggaagc ctcgacctgg gtcgcctttg cttttggcgg tattgtggcg
       61 atgttttccg gttatgccta tgcgcgtctg ggggcgagct atcccagcaa tggcggcatt
      121 atcgacttct ttcgtcgcgg attaggcaac ggcgtctttt cgctggcgct ctcgttactg
      181 tacctgttga cgctggcggt gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc
      241 gtgcagtttt tgcatgaagg cagccaggag gagcacctta ttttgctcta cgcgttgggg
      301 atcattgcgg tgatgacgct tttcaactcc ttaagcaacc atgcggtagg gcggctggaa
      361 gtgatcctcg tcggcattaa aatgatgatc ctgttattgc tgattattgc cggtgtctgg
      421 tcgctgcaac cggcgcatat ttccgtctct gcgcccccca gctccggtgc gttcttctcc
      481 tgtatcggga taaccttcct tgcctatgcg ggctttggca tgatggcgaa cgcggcggat
      541 aaagtgaaag atccgcaggt cattatgccg cgggcgtttc tggtggcgat tggcgttacc
      601 acgttgcttt atatctcgct ggcactggtt ttgcttagcg atgtatcggc attagagtta
      661 gaaaaatatg ccgataccgc cgtagcgcag gctgcttctc cgctgctcgg acatgtgggt
      721 tatgtgatcg tcgtcatcgg cgctttactg gcgacggctt cagccattaa cgcgaacctg
      781 ttcgccgtgt ttaacatcat ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg
      841 aataaatccc tgtggcggca gagtacctgg ggcaacatca ttgtcgtggt gttgattatg
      901 ctgatgacgg cggcgctaaa tttaggctca ctcgccagtg ttgccagcgc cacctttttg
      961 atttgctacc tggcggtgtt tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg
     1021 ttgccgattc ttatcgttgg tacgttggtg atgttgttgg tgatcgttgg ctttatctac
     1081 agcctgtggt cccagggtag ccgtgcgttg atatggatta ttggctcact cttactcagc
     1141 cttattgtgg caatggtcat gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt
     1201 acttcctgta ttattgtggt ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat
     1261 atctgttgaa accctttaac aaagcacagg aggcgttgcg cgaacgatga acatcagcga
     1321 tgtagcaaaa attaccggcc tgaccagcaa agccattcgc ttctatgaag agaaggggct
     1381 ggtgacgccg ccgatgcgca gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa
     1441 cgaactgacc ttactgcgcc aggcacggca ggtgggcttt aacctggaag agagcggcga
     1501 gctggtgaat ctgtttaacg acccgcagcg gcacagcgcc gacgtcaaac ggcgcacgct
     1561 ggagaaggtg gcggagatcg aacgacacat tgaggagctg caatccatgc gcgaccagct
     1621 gctggcactg gcgaatgcct gccctggcga tgacagcgcc gactgcccga ttatcgaaaa
     1681 tctctccggc tgctgtcatc atcgggcagg gtgattaaga cgagacggct cggatatgca
     1741 gggttatccc ttctatcgca atgacttcaa catgcgtacc tgcaccgaga tcctcgctgg
     1801 cgctgacagg ccatgaactg tcaccgacgc gcatatgacc gcgcccgttg accagcggag
     1861 attccagcac aaaacgtcgg ccaatcagct gctgcccgcg ctggtttaaa tgactatcgc
     1921 tgtgcttttg ttcgcgcacc cgccgcgaca accatttcca ccacagccag gcggcgagca
     1981 gcgtcaggat ggcaaacatc accccttgcc actcccaacc cagcggcacc agccagacca
     2041 ccaggccggt aatcaccgct gacacgccgc tccacagcaa ataaccattt ccgcccagca
     2101 tctcggctgc cagcagcaac ccgccgagac tgagccagaa aatatgtgga tgaacgacca
     2161 ttaactccat catggctgag tccgtttgtt ggcgctgtct ttcaccagct cggcaatccc
     2221 ggcaatcgac cccatcaggc tgctggcctc taatggcatc atcactactt tgctgttact
     2281 ggaggaaccg atctgctgta acgcttcggt gtatttctgc gctacgaagt agttcaccgc
     2341 ctgaatatca ccggaggcaa tggcttcaga taccattttg gtggcgcggg cttctgcttc
     2401 ggcagaacgt tcacgcgctt cggcctgtaa gaatgccgac tgacgttcac cttccgcttt
     2461 cagaatttgc gactgttttt caccttccgc tttgagtatt tccgcctgac ggatcccttc
     2521 cgcttcaagg atgtaagcgc gtttggtacg ttccgctttc atctgcgcgt tcattgaaga
     2581 gataagctct gccggtgggc gcacgtcgcg aatttcaata cgggtgactt taatccccca
     2641 cgggttggtg gcttcatcga caatacgcag caggcgtgag ttgatgctgt cgcgctgaga
     2701 gagcatttcg tcaagttcca ttgaacccaa cacggtacgg atgttagtca tggtcaggtt
     2761 gatgatcgcc agctccagat tgctgacttc ataagccgcg cgcggcgcgt caatcacctg
     2821 aataaaacag actgcgtcga tggtaacgtt ggcgttatct ttcgagataa cttcctggga
     2881 agggatatcg agcacttgct ccatcatatt gatcttgcga ccaatgcgat ccataaacgg
     2941 caccaccaga ctgagccccg gctgtaacgt tttggtatag cgaccaaaac gttctaccgt
     3001 ccattgatag ccctgcggca cgattttgac gcccgcgccg acaatgacca gcgcgacaaa
     3061 aatgagaatc gggataaaga taagcatcgg aaaaacctcc tgttgtaccg tccataatca
     3121 gcaaaattgc tgcttgatta aacaaattat acctgattac tgaaagagag ttccccctta
     3181 ttcctgcgaa ggataaactg tttttagtaa aaatcagaaa aagggaacag tgatgcagga
     3241 aaatagtcct ttgcttcagc tacaaaacgt aggatatctg gcgggtgatg cgaagattct
     3301 taataacatc aatttttcgc tgcgtgctgg cgaatttaag ttaattaccg gtccttccgg
     3361 ttgtggcaaa agtacgctgc taaaaatagt tgcttcattg atcagcccaa ccagcggaac
     3421 gttactgttt gaaggtgagg atgtcagcac actaaagcca gaaatctacc gccagcaagt
     3481 ctcttactgc gcccagacgc cgacgctgtt tggcgatacg gtatacgata atctgatctt
     3541 tccctggcag atccgtaacc agcagcctga cccggccatt tttctcgatt ttctcgaacg
     3601 cttcgctttg ccggacagca ttttgacgaa gaatatcgcc gagctatctg gtggtgaaaa
     3661 acaacgcatc tcattgattc gtaacctgca atttatgccg aaggttttat tgctggatga
     3721 aataaccagt gcgctggatg aaagtaataa acataacgtc aatgagatga tccatcgtta
     3781 tgtgcgcgag caaaatattg ccgtgctgtg ggtgacacac gataaagacg aaattaatca
     3841 tgcggataaa gtgattacac tgcaaccgca tgccggagaa atgcaggaag cacgctatga
     3901 actcgcataa tattactaac gaatcattag cactggcatt aatgctggtg gtggtggcaa
     3961 tcttaattag ccataaagaa aaactggcgc tggagaaaga tattctctgg agcgtcgggc
     4021 gagcgataat tcagctgatt attgtcggct atgtgctgaa gtatattttc agcgtggatg
     4081 atgccagcct gacattattg atggtgttat ttatctgctt taatgcggcg tggaacgcgc
     4141 aaaaacgcag taaatatatt gctaaagctt ttatctcatc gtttattgcc attacggtcg
     4201 gggcgggaat taccctggcg gtgctgattc tctcagggtc gattgaattt atcccgatgc
     4261 aggtgatccc tatcgccggg atgattgccg gtaacgccat ggtagcggtg gggttgtgtt
     4321 acaacaattt agggcaacgg gtcattagcg aacagcaaca gattcaggag aaactgagtc
     4381 ttggtgcgac gccgaagcag gcttcagcga tattgattcg cgacagtatt cgcgcggctt
     4441 taattccgac ggtcgattca gcaaaaacgg ttggcttagt gagtttacca ggaatgatgt
     4501 ccgggctgat atttgccggg attgatccgg tgaaggcgat taaatatcag attatggtga
     4561 cctttatgct gctctcaacc gccagcttgt cgaccattat tgcctgctat ttaacctatc
     4621 gtaagtttta taattcgcgc caccagttgg tggtgacgca attgaagaag aaatgatgcg
     4681 atgccggatg cgcagcatcg catccggcgt tgtggttcat gtgccgatca acggaatatc
     4741 aatacaacaa tgcatacagc tggcggcgat acttcgacgc cagtgcatca cccgtaccca
     4801 gcgcagcgag gatctcctgg aacgttttac gcgtctgacc gtctgcggcg gtgagatctt
     4861 tacgcagatg cccgaacagc aactccagcg cctcttcatt gcgcccaacc tgatgcagtt
     4921 gcagcgccag ttgcgtcgcc agtgcggcat cttctggatt ctccgccacc tgctgttgca
     4981 actgttgaat ttccggagta tcagccgcct gcttcagcag ttcgatttgc gccaccagcc
     5041 cctggtagcg ggtgtcctga tcctgcaacg gaatggtttt cagcaccgct tccgcatctt
     5101 cagaacggtt cagcgcaatc agcgtttctg ccagcagcag gccgatctcc ccgttctgat
     5161 tcgacaactg ccaggcgtct ttcagcaatg gcagggcatc ggtgtaattg ctttcctgca
     5221 tcagttgcat cgcctgctgc gctttcagct cttcttcgcg cggcagcact ttatccagca
     5281 gggcgcggat cgcctcttcc ggttgcggcc cctggaagcc atctaccggt tgcccgttct
     5341 ggaacagata cacggtcgga atcgcgcgca gaccaaactg ggcggcaatc atctgctccg
     5401 cgtcgcagtc cagcttcgcc agaataaatt gcccgttgta ctgcgccgcg aggctttcca
     5461 ggatcggggt taactgcaaa cagtgctggc tacgttcaga ccaaaaatag aacagcaccg
     5521 gagtggtcat cgactgttca agaacctgtt gcaggttaga ttcgttaatg ttgacaatat
     5581 tttctacgga catggagtcg ctctctgttg tcgatatttt ctttgacatg ggggcttaag
     5641 cgcgcgcttc aactcacccc tgcaatattt tgtccatcac gcgccccggc agcaggcgct
     5701 taagcaccat tactgcccag gtcaccagcg ttaccggata gcgcatcttc ggcttctcgc
     5761 taataaaagc atggcgtact ttgtccacca ccgcttccgg tcccaacgta aagcgggcgg
     5821 cgatgccggg attttcgact ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc
     5881 gagtacgaat gggaccgggt tcgatcaggc tgactttaat tccgctgtgg cgtagctcca
     5941 tgcgcagcgc gtctgaccac gcctccagcg catatttact ggccgcgtaa gcgccacgac
     6001 ccggcgtgga gattaatccc atcaccgatg atgtcatcac aatacgccct tcaccgtgcg
     6061 gtaacatcgc gggtaacagg cgcatggtga gctggtgtgc gccgaaaaag ttggcggaaa
     6121 actgctgttc catctgcgca cggctgatgg tggaaagggg gccatacatg ccgaatccgg
     6181 cattgttaaa gatcccatac agacaattat cggtcagggc gatcacctcg tctgctgcgc
     6241 gatcaacact ttctggtgaa tccagatcga tcaacacgcc ggtaaatccc atgttgttca
     6301 tacgctcaac atcatccggt tttcggcaac ctgccagcac atgaaaaccc tggcgtttta
     6361 attcgagcgc gctttccagg ccaattccac tggaacatcc ggtaattaag accgattttt
     6421 gcataacttt acctgtcagg atctccgttg ctttatgagt catgatttac taaaggctgc
     6481 aactgcttcg ccatccagtc ggcaataaac ggctgggcgt cgcggttggg atgaataccg
     6541 tcatcctgca tccattgtgg cttgaggtag acctcttcca taaaaaaggg cagcagcgga
     6601 acatcaaact ctttggcgag tttggggtaa atggcgctaa aggcttcatt ataacggcga
     6661 ccatagtttg caggcagacg tatttgcatt aacaatggtt cagcgttggc ggctttgaca
     6721 tcctgcaaaa tctggcgcag cgtttgctcg gtttgctgtg gctgaaaacc acgcaaaccg
     6781 tcattgccgc ccagttcaac cagcacccag cgcggctgat gctgtttcag cagagccgga
     6841 aggcgcgcca gtccttgttg cgaggtgtcg ccgctgatgc tggcattaac taccgacgtt
     6901 ttactctgcc acttatcatt caacaaggca ggccaggccg cgctggcaga cattcgatac
     6961 ccggcgctca ggctatcacc cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt
     7021 aacaggacca ggaacaggaa gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc
     7081 ttaagaagtc cgtcggtcag ggggagcatg aactctccat cctcaccgga gttgagctgg
     7141 ttgtcaaacg tggcgagacc atcgcactgg tgggcgagtc gggatcgggt aagtcaacct
     7201 tgctggcgat cctcgccggg cttgatgacg gcagcagtgg cgaagtgagt ctggtgggac
     7261 aaccgctaca taatatggac gaagaagcgc gggcaaagtt gcgcgcgaag cacgtcggct
     7321 ttgtttttca gtcatttatg ttaattccta cccttaacgc gctggaaaac gtcgagcttc
     7381 cggctctgct gcgcggtgag agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg
     7441 aacagttagg gctgggtaaa cgtctggatc atcttccggc acagctttcc ggcggtgaac
     7501 agcaacgagt ggcgctggca cgagccttta atggtcgacc tgatgtgctg tttgccgacg
     7561 aacccaccgg caaccttgac cgccagacgg gcgataaaat tgccgacctg ctgttttccc
     7621 tcaaccgtga acatggcacc acgttgatta tggtgaccca cgacctgcaa ctggcggcac
     7681 gctgcgaccg ctgcttacgg ctggtgaacg ggcagttgca ggaggaagca tgattgcacg
     7741 ttggttctgg cgcgaatggc gttcgccgtc gctattaatt gtctggctgg cgctaagcct
     7801 ggcggtggcc tgcgtgctgg cgctgggcaa tatcagcgat cgcatggaga agggcttaaa
     7861 ccaacaaagc cgggagttta tggcgggcga tcgggcgttg cgcagttcgc gcgaagtgcc
     7921 gcaagcatgg ctggaggaag cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt
     7981 cgccaccatg acatttgcag gcgacacacc gcagctggcg aacgtcaaag cggtggatga
     8041 tatctacccg atgtatggcg atctgcaaac taatccccct ggcctgaaac cgcaggcggg
     8101 cagcgtattg ctggccccac gcctgatggc gctgcttaac ctgaaaacgg gcgacaccat
     8161 tgacgtgggc gatgccacct tgcggattgc cggagaagtg attcaggaac cggattccgg
     8221 ttttaacccc ttccagatgg ctccgcgtct gatgatgaat ctggcggatg tcgataaaac
     8281 cggagccgtg cagccgggga gtcgggtcac ctggcgttat aaattcggcg gcaacgagaa
     8341 ccagctcgac ggctatgaga aatggttgtt acctcagctt aaacccgaac aacgctggta
     8401 cggtctggaa caggacgaag gcgcgctggg gcgatcgatg gaacgctcgc aacagttcct
     8461 gctgctttcg gcgcttctga ccttgctgct ggcagtggca gcggtggcgg tagcgatgaa
     8521 tcattactgt cgcagtcgct acgatctggt ggcgatcctc aaaacgctgg gggcagggcg
     8581 agcgcaactg cgtaagcaaa tcgtcggtca gtggttgatg gtgctggcgc tttcagccgt
     8641 taccggtggg gcgataggcc tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt
     8701 tctgcctgct gcactaccgc cagccagcct ctggccgtgg ctgtgggcgc tgggtaccat
     8761 gacggtcatc tcgctgctgg tggggctacg accgtaccgc ctgttgctgg caacgcagcc
     8821 tttacgcgta ttacgtaatg atgtggtagc gaacgtctgg ccgctgaagt tttatctgcc
     8881 gattgtcagt gtggtggttg tgctgctgct cgccggatta atgggtggca gcatgctgct
     8941 ttgggcggtg ctggcgggcg cggtagtact ggctttgctg tgcggtgtgc tgggctggat
     9001 gctgctgaat gtacttcgcc gcatgacgct gaaatcgcta cctctgcgcc tggcggttag
     9061 ccgcctgtta cgtcagccgt ggtcaacatt aagccagctt tcggcatttt cgctctcctt
     9121 tatgctgctg gcgctgttgc tggtattgcg tggcgatctg cttgaccgct ggcagcagca
     9181 gctacctcca gaaagcccga actacttttt aattaacatt gccacagaac aggtagaacc
     9241 gctaaaagcg ttcctcgcgg aacatcagat agtcccggaa tcattttatc cggtagtgcg
     9301 ggcgcggctg acggcgatta acgataagcc gacagaaggt aatgaagatg aggcgcttaa
     9361 tcgcgaactc aatcttacct ggcaaaatac gcggcccgat cataatccga ttgtcgccgg
     9421 taactggccg ccaaaagccg atgaagtgtc gatggaagag gggctggcga aacgcttaaa
     9481 cgttgccctc ggtgataccg tgacttttat gggcgatacc caggagttcc gcgctaaagt
     9541 gaccagcctg cgcaaagtgg actgggaaag tctgcggcct aatttctatt ttattttccc
     9601 ggaaggggca ttagacgggc aaccgcagag ctggcttacc agtttccgct gggagaatgg
     9661 caacggcatg ttgacgcaac tcaaccgcca gttcccgacc attagcctgt tagatattgg
     9721 cgcgatttta aaacaggtcg gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat
     9781 ggtggtgctg gtcaccgcct gcggtatgtt gctgttgctg gcacaggtgc aggtgggaat
     9841 gcgccagcgt catcaggagc tggtggtatg gcgcacactc ggtgcgggga aaaaactgct
     9901 gcgcacgaca ttgtggt
//
LOCUS       DAZFVO020000174         9830 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFVO020000174 DAZFVO020000000
VERSION     DAZFVO020000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.175"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(155..328)
                     /locus_tag="ACHKNB_RS21150"
                     /old_locus_tag="pgaptmp_004230"
     CDS             complement(155..328)
                     /locus_tag="ACHKNB_RS21150"
                     /old_locus_tag="pgaptmp_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197629.1"
                     /translation="MQVDIISPVTDPYACAHQALLALINAGKIENSAMCFEELKQLVR
                     LYEYIKKQSQDTA"
     gene            868..1056
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS21155"
                     /old_locus_tag="pgaptmp_004231"
     CDS             868..1056
                     /gene="dicB"
                     /locus_tag="ACHKNB_RS21155"
                     /old_locus_tag="pgaptmp_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HGU4197630.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            1053..1241
                     /locus_tag="ACHKNB_RS21160"
                     /old_locus_tag="pgaptmp_004232"
     CDS             1053..1241
                     /locus_tag="ACHKNB_RS21160"
                     /old_locus_tag="pgaptmp_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HGU4197631.1"
                     /translation="MTSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            1337..3808
                     /locus_tag="ACHKNB_RS21165"
                     /old_locus_tag="pgaptmp_004233"
     CDS             1337..3808
                     /locus_tag="ACHKNB_RS21165"
                     /old_locus_tag="pgaptmp_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HGU4197632.1"
                     /translation="MNKIFICAAIPDEQAIEEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NITFDNLAPEVQNTVMVKFDACENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPAAKWPEIQAEMRIWKKRRESERKETGKYISVVDIARS
                     RVNQQNTENAAEKTGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALAYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDFE
                     NQNQPESEQNDQKAEQNSPNALQNEPETKQPEPEEQQEPEKVCTACGQIGGGNCPDCG
                     AVMGDATYQETFDEKNRAEVQEDDPEEMEGAEHPNNENAGNDQHHTSDSETGEAADPL
                     IAVNGHHVITSTSRVWIHLSVDLETMGTNPDAPINSIGGTFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGCFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            3873..4121
                     /locus_tag="ACHKNB_RS21170"
                     /old_locus_tag="pgaptmp_004234"
     CDS             3873..4121
                     /locus_tag="ACHKNB_RS21170"
                     /old_locus_tag="pgaptmp_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HGU4197633.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            4099..5238
                     /locus_tag="ACHKNB_RS21175"
                     /old_locus_tag="pgaptmp_004235"
     CDS             4099..5238
                     /locus_tag="ACHKNB_RS21175"
                     /old_locus_tag="pgaptmp_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HGU4197634.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERY
                     ENGEIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRK
                     VLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQR
                     GMLLALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTQDDVV
                     SSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFH
                     EQRSLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(5284..5937)
                     /gene="ompW"
                     /locus_tag="ACHKNB_RS21180"
                     /old_locus_tag="pgaptmp_004236"
     CDS             complement(5284..5937)
                     /gene="ompW"
                     /locus_tag="ACHKNB_RS21180"
                     /old_locus_tag="pgaptmp_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HGU4197635.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            6177..6266
                     /locus_tag="ACHKNB_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /pseudo
     CDS             6177..6266
                     /locus_tag="ACHKNB_RS21185"
                     /old_locus_tag="pgaptmp_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            6294..7037
                     /gene="yciC"
                     /locus_tag="ACHKNB_RS21190"
                     /old_locus_tag="pgaptmp_004238"
     CDS             6294..7037
                     /gene="yciC"
                     /locus_tag="ACHKNB_RS21190"
                     /old_locus_tag="pgaptmp_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HGU4197636.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     ITQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            7067..7606
                     /gene="yciB"
                     /locus_tag="ACHKNB_RS21195"
                     /old_locus_tag="pgaptmp_004239"
     CDS             7067..7606
                     /gene="yciB"
                     /locus_tag="ACHKNB_RS21195"
                     /old_locus_tag="pgaptmp_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HGU4197637.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            7711..8109
                     /gene="yciA"
                     /locus_tag="ACHKNB_RS21200"
                     /old_locus_tag="pgaptmp_004240"
     CDS             7711..8109
                     /gene="yciA"
                     /locus_tag="ACHKNB_RS21200"
                     /old_locus_tag="pgaptmp_004240"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HGU4197638.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(8149..8868)
                     /gene="tonB"
                     /locus_tag="ACHKNB_RS21205"
                     /old_locus_tag="pgaptmp_004241"
     CDS             complement(8149..8868)
                     /gene="tonB"
                     /locus_tag="ACHKNB_RS21205"
                     /old_locus_tag="pgaptmp_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HGU4197639.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMAAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            8959..>9830
                     /locus_tag="ACHKNB_RS21210"
                     /old_locus_tag="pgaptmp_004242"
     CDS             8959..>9830
                     /locus_tag="ACHKNB_RS21210"
                     /old_locus_tag="pgaptmp_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4197640.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPAGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTKMVPGWIDPDTFKCNEHAETVRLIFKLLLDGESLHNIARHLQSNDI
                     KSFSRRKDANGFSVHSVRTILRSETTRGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPAS"
BASE COUNT         2710 a         2271 c         2388 g         2461 t
ORIGIN      
        1 aaagcgatga ttatttaaaa accataatta aaacaactca gctttctgaa gagctaccga
       61 agaaattgcc ataataatat gtttttctta tagaggggtt agaaatatgg gccagcatta
      121 tagcaggcac atataacaga aagtatttta aatattacgc cgtatcttgt gattgttttt
      181 taatatattc atacaagcgc acaagttgtt tcaactcttc aaagcacata gcggaatttt
      241 caatttttcc ggcgttgatt agtgccagaa gagcctgatg tgcgcaagca tatggatcag
      301 ttacagggct aataatatca acttgcatat tatcctccat agaggttccg ggttaaggat
      361 ggagaccaac acgctgtcac gtgtggtcgt gcgccggaca cggataagaa tccggcactg
      421 acagtttgct gaaagaacat atccctgaaa agtcagggca taacacgaaa gcgcacggcg
      481 agaatataag gcttgtcgtt aaatttaatt cgatcgtgcg cttccggttg tggcaatccg
      541 cgaaatggcg cggcggtaag tatggctggg gcttcctcca ttgctccaga aaatgcaccg
      601 ggttgtcagg ttgaccatgc gcctgagtga caacaccgcc acaacaacct ctgttatcac
      661 ttttctggtg attcggcgga aatggatatc cgccattttt aaagtgtatt ttgtgatgcg
      721 gtgaatgcgg ctaagcgcac gcggaacagt taaagctaaa aacagcgtta tgggtgattc
      781 tgtattccgg cgttaattgt taactggtta acgtcacctg gaggcaccag gcaccacatc
      841 acaaaattca ttgttgagga cgcgataatg gaaacgtcac taccaaacgt taatacgtct
      901 gaagggtgtt ttaatattgg tattctgctc agtaaccggg agtttactga agacgccatc
      961 aggatgagaa aatatgagcc ttatcttctc aatgataatt ccatactctc cagaattgcc
     1021 cttcttaaac ttggtatttt cggggggcag cagtgacttc tgcatttgca ctgatgatga
     1081 cggtttttct tataacaggt gagccacaga atgtgattac cggaatttat gccagtaaag
     1141 aatcctgcca tcaggcaaga gacgagcaaa aaatttccgg tgaatgtctc ccgttaaaca
     1201 aagtatcgct gtacctgaat aacgaaacac cggctggata acccgccagc catattaacg
     1261 ccataccaac ggattaaaaa tgccagcaat ggcagggatt cgttcaccct gaaatctgta
     1321 atgaggttaa aacacaatga ataaaatctt tatttgcgct gctattcctg acgaacaggc
     1381 catagaagaa gatagcgctg ttgcggtggc cactgccatt gaagccggtg atgagcgtcg
     1441 cgcacgcgca aaatttcact ggcaatttct ggagcaattc ccggcagctc aggactgcgc
     1501 ttataaattt attgtctgcg aggataaacc cggcataccc cgccctgccc tcgattcctg
     1561 ggataccgaa tatatgcagg aaaaccgctg ggatgaggag tctgcgtcct ttgtcccggt
     1621 cgaacccgaa tccgatccga tgaacatcac ttttgacaat ctggcccctg aagtacagaa
     1681 caccgtcatg gttaagttcg acgcatgtga aaacatcact gttgatatgg tgattagcgc
     1741 tcaggaattg ttgcaggaag acatggcaac attcgacggc catatcgttg aagcgttgat
     1801 gaagatgcca gaagttaacg ccatgtatcc ggagcttaag ctgcatgcca tcgggtgggt
     1861 taagcataaa tgtaaacctg ctgctaaatg gcccgaaatt caggcagaga tgcgcatctg
     1921 gaaaaaacgt cgcgaaagtg aacgcaagga aaccgggaaa tacatatctg tcgttgatat
     1981 cgcccgttcc agagtaaacc aacagaacac tgaaaacgct gctgaaaaaa ccggggctgt
     2041 cactgttgcc gttcgccgcg aatacaaaca gacatggaaa acactcgaca atgaactggc
     2101 ctgcgccctc tggcccggtg atgtggatgc aggaaacatt gacggtacca tccatcgctg
     2161 ggcgacaaat gaggttatcg acaaggatcg cgaagactgg aagcgtatct cagcatcaat
     2221 gcgcaaacag cccgaagcac ttgcctatga ccgtcagact atttttggcc ttgttcgcga
     2281 acgtccgatc gatattcaca aagatcccgt tgcactgaac aaatacatca gcgaatacct
     2341 gacgacaaag ggcgtgtttg aacatgaaga aacagaccag agctctgctg atgctatcct
     2401 gtcgtcagca gcacaaactg atccagtgga gacggcggaa tccaattctc aaaaaaatga
     2461 aatcctggtg gaagctgaac catctgtaga gcgtgaagga ccgttttatt tcgtctttac
     2521 cgataaggac ggggaaaaat acggcagggc aaacaaactt tctggcctgg acaaggcgct
     2581 ggctgccggt ggtaccgaaa tctcaaaaga agaatatttt gcccgaaaaa atggcacata
     2641 tacgggctta cagcaaaata cagataccgc agaagattca gaacaaccag agccggtaaa
     2701 agttaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacac
     2761 cgataaattg cttgctgcat cacgtggtga atttgttgaa gggattagcg acccgaatga
     2821 tccgaaatgg gttaagggga ttgaaacccg cgattttgag aaccagaacc agcccgaatc
     2881 ggaacaaaac gaccagaaag cggaacaaaa cagcccaaat gcgttacaaa acgagccaga
     2941 aacgaaacag cctgaaccag aagagcaaca agaaccggaa aaagtctgca ccgcctgcgg
     3001 tcagatcgga ggcggcaact gtcctgactg tggcgcggtg atgggcgacg caacatacca
     3061 ggaaacattc gatgaaaaaa atcgggctga agttcaggaa gatgatccgg aggaaatgga
     3121 aggcgctgaa catccgaaca atgagaatgc tggtaacgat cagcatcaca ccagcgatag
     3181 tgaaactgga gaggcggcag atcccttaat tgcggtgaac ggtcatcacg ttatcacatc
     3241 caccagcaga gtgtggattc acctgtcggt cgaccttgaa acgatgggaa caaatcctga
     3301 tgcgccaatc aactctattg gcggtacatt ttttgatccg gcaaccggag agatgggacc
     3361 tgaattcagt aaagctatcg atctggaaac ctccggcgga atcatcgacc gtaaaacaat
     3421 aaagtggtgg gcgaaacgat cacgcgaagc acaatccgct atttttaccg atgaaatctc
     3481 gttagatgtt gctctccggc tattcataga atttatcgaa aaaaactccg gcgggtgttt
     3541 tgtccaggta tggggaaatg gagccaactt cgacaacgta attttacgcc gttcatatga
     3601 acggcagggg atcccctgcc cgtggcttta ctacaacgat cgcgatgtac gcacaatcgt
     3661 tgaactgggt aatgccattg gctttgatgt cagaatggct attccattcg aaggcgtacc
     3721 ccacaatgcg ctggacgatg cccgtcacca ggcaaaacaa gtttcagcaa tctggcaaaa
     3781 actgatcccg agtcaggctg atttttaatg ttcaaccccg gtcgtcgtcc gcaagctata
     3841 gtggcggcgg ccatgattag cgaacaacgc tcatggcaag acttattctg ctcactgagt
     3901 gggcaaaaga ggaattcagt gaaccggtcc ctactccggg cacgttaagt aaatacgcta
     3961 aagccggaat gatatttcct ctccccaaaa aagttggaag acactggcga gtggatcctc
     4021 gagctcgctt tgtcggaatg gtaaacaagc cggaagtgat cgccacagat caccctgctt
     4081 tgaagaggat actggaagat ggcgcgccca cgaaaatata aaactgaagt tccgggatta
     4141 tctccgtatt ttgacaaaag aaataacaaa gtttactggc gttacaggca tcccataaca
     4201 ggaaaaaatc acgggctcgg cagtattgac cagaaacagg cagaagctat tgcagcagaa
     4261 gcgaacagcc gtcttgccag gcaacaaatg gaacaaatgg aacaaatgct cactctgcag
     4321 gagaaaatta ttcgtgatac cggtggttca tcaaccgttt ctgtttttct gaatagttac
     4381 cgaaaaattc aacaggaaag atatgaaaac ggagagatca aactcaacac actgaaacag
     4441 aaagcatccc ctctcagggt atttgatgaa cgttttggta ccagaccttt agatgccata
     4501 accgtaaaag atgtggtatc ggtgctggaa gattacaagg caaaaggaca taacagaatg
     4561 ggacaaattt tcaggaaagt actgattgat gttttccggg aggcccagca aacgggcgat
     4621 gtcccgcctg gctttaaccc tgcagaatcg gcaaaaaaac cacaggtacg gatatcacgg
     4681 caacgactga cctttgatga gtggatgatg atttataatg cagcggaaaa ggatggttac
     4741 tttttacagc gtggtatgct gctggcactg atgacaggcc agcgcctttc agatatttgc
     4801 aaaatgaaat tttcggatat cagggatggt tatcttcatg tcgaacagca aaaaacagga
     4861 acccggattg ccatccctct ggctctgcgt tgcgataaat taaatctcac ccaggatgat
     4921 gtggtgtcat cctgtcgcga ttgcgttctt agtccgtggc tattgcacca ccatcatgct
     4981 aaagggacag ctaagcgcgg cgggatggtt aagccagcaa cgttaaccgt tgcatttaaa
     5041 aaagcccggg attctgtgga ttacaactgg cgtgctaatg gcaccccacc ctctttccat
     5101 gagcagagat ctttatcaga acgattgttc agagaacagg gaattgatac caaaattttg
     5161 ctgggccatt cgaatcaaaa aatgaccgat atttacaacg acgcacgcgg taaggaatgg
     5221 aaaaaactgg tcatttgatg accagttttg cagaagagtt ttgcagaggt tttgcagagc
     5281 aaattaaaaa cggtaccctg cagagaacat aaacacccac ggatcaaggc gtacattgtc
     5341 gctataactg cctttagcca gtttacgacc atcagattta tacttagccg tggtatcgat
     5401 atccatgtac cacactgaca tgttaaccaa ccagtcacgg ttaatcagat aatcaacccc
     5461 cacctgcccg gcagctcccc aggaatcttt cagactgaga tcggaaagcc ctgcctcttt
     5521 gccatgatcg ttaaatccat tatcaaagaa ggtggtgtag ttaataccgg ccccaacgta
     5581 aggacggaat ttgctgctgg catcaccaaa ataccactgc gccatcagtg ttggcggcag
     5641 atgatgaacg gttgcaatat cgccggtcgc ccgggtgccg attttatggc ggaacggcgt
     5701 cgctgccagt aattccacac caatgttgtc ggtcgccata taagtaaacg tcaggcccag
     5761 ttgagtgtta ttggtcacgc tgaatccacc cagacttcct aacgtaccac cagcaccttc
     5821 tgttggacgt acggttgcag aacctgcacg cataaaaaat tcgcctgctt catgcgcaaa
     5881 ggcactgcca gagagaagag ttgttactgc caaagccgcc actgttaact ttttcatatc
     5941 cgctccgtcg ttatggttat aaaaacgtga cgaatatacc tacattggag taacaaatga
     6001 tcctacatag atcacattac atatgataat tcaccattta ttgatctgga ttaatttaga
     6061 agttgcattg agaatcctgg ttaacgtgat ttaaatcaat ttttaaatat tttcgctacg
     6121 caaatattaa ctcgttcgcc acttgccgcg gtttgcccac gcctgaaggc cagcaattac
     6181 ctccgcttta caaagatatg cttttccata caagacctga tgctgccacc cgaggcttaa
     6241 ccagggtaca attgcccgct aattaacacc tgcataaaac tcaaggagag tgcatgtcta
     6301 tcacggcgca gtccgtatac cgtgacaccg gaaatttctt ccgtaatcaa tttatgacca
     6361 ttctgttggt atcgttgcta tgtgcgttta tcacagtggt gttagggcat gttttctcac
     6421 ccagtgatgc acagcttgcg cagctcaatg acggcgtgcc cgttagcggc agtagtgggt
     6481 tgttcgacct ggttcagaat atgtcaccgg aacagcaaca aattttgctg caggcttcag
     6541 cggcgtccac tttttcagga ttaatcggta acgccattct cgccggaggc gtaatattaa
     6601 ttacccagct ggtgtctgcg ggtcagagag tcagtgcgct gcgagctatt ggtgccagtg
     6661 cgccgatatt gcccaagtta tttattctga tttttctgac taccctttta gtacagattg
     6721 gcatcatgct ggtggtcgtt ccgggaatta tcatggccat tttactggcc ctggcaccgg
     6781 tgatgctggt acaggacaaa atgggcattt ttgcctcgat gcgtagcagt atgcggctga
     6841 cttgggcgaa tatgcgtctg gtggcacccg cagtactgag ctggttgctg gcaaaaacac
     6901 tgttgctgct ttttgcctct tcttttgccg cattaacccc ggaaattggt gccgtactgg
     6961 cgaacacctt gagcaacctg atttctgcca tattgctcat ctatctgttc cgcctgtata
     7021 tgttgattcg ccaataacct taagcatttg attacggaat cgtaaaatga agcagtttct
     7081 tgatttttta ccgctggttg tctttttcgc gttttacaag atttatgaca tctatgcggc
     7141 tactgcggcg ctgatcgtcg ccacggcgat tgtgcttatc tatagctggg ttcgctttcg
     7201 taaggttgag aagatggccc tgatcacttt tgttctggtg gtcgtcttcg gtggcttgac
     7261 gctgttcttc cacaatgatg agtttataaa atggaaggtt actgtcattt atgccctgtt
     7321 tgcgggtgcc ctgttagtca gccaatgggt gatgaaaaag ccgctaattc agcggatgct
     7381 gggtaaagaa ctcacgctgc cgcaaccggt atggtcgaag ctgaatctgg cctgggctgt
     7441 tttctttatc ctttgcggtc tggcaaacat ctacatcgca ttctggctgc cgcaaaatat
     7501 ttgggtcaac tttaaagtct ttggcctgac cgcccttacc ttaatcttca cattgttaag
     7561 cggtatctat atctaccgcc acatgccgca ggaagataaa tcctaaccag actgccagtc
     7621 cttcggggct ggcgctgtat ctcctctgtt ctcctcagga aaatcatagt agcatcgcgc
     7681 ctgtgatttt ccttttaagt cggttttacc atgtctacaa cacataacgt ccctcagggc
     7741 gatcttgttt tacgtacttt agccatgccc gccgatacca atgccaatgg tgacatcttt
     7801 ggtggttggt taatgtcaca aatggatatt ggcggcgcta ttctggcaaa agaaattgcc
     7861 cacggtcgcg tagtgactgt gcgggttgaa ggaatgactt tcttacggcc ggttgcggtc
     7921 ggcgatgtgg tgtgctgcta tgcacgctgt gtccagaaag ggacgacatc ggtcagcatt
     7981 aatattgaag tgtgggtgaa aaaagtagcg tctgaaccaa tcgggcaacg ctataaagcg
     8041 acagaagcat tatttaagta tgtcgcggtt gatcctgaag gaaaacctcg cgccttacct
     8101 gttgagtaat agtcaaaagc ctccggtcgg aggcttttga ctttctgctt actgaatttc
     8161 ggtggtgccg ttaattttaa acaggatatt caccacaatc ccactgcctg gcttacccgg
     8221 ctcataacgc catctgcgca tcgcattttt cacctcacgc tcaaacatgt tcgcaggctt
     8281 ggctgagagg atttgtacgt tatccacgcg accatccggc gtgacgtcaa atttaacttt
     8341 aacctgccct tcaatgcgca atgcctgtgc tcgtgccgga tactgcggct gattacggct
     8401 taatgcgcgt ggtcctgaag ccacactggt aaccggcttg ctggttgcag ccgttgccgt
     8461 acttgatgtg gggcgcgcgg gtgccgtatt ttcaaacggt gatgccggac gcgactctac
     8521 gggtttgata tcgcgttttg gctgctcctg tacctttttc accggcttcg gttttggctt
     8581 aggtttcggc ttcggctttt caatgaccac cggtgcttct tttggcggtt cggggatcgg
     8641 ctcaggttcc ggttctggct ctaccaccgg ctccggtggc ggctgaacgg cttgtggcgg
     8701 ttcgagatca gcaggcgcag ccatcgtgac agaaattggc tgcgcaggcg caggtagttc
     8761 aataacctga tgtaccgagg tatagagcag acccgccaca acagcaccat gaatgcagac
     8821 cgaaagtaac gtcggccagg ggaagcggcg aggtaaatca agggtcattg tattgtagct
     8881 attcggcatt gtagcattag ccataacgtt tataagttca tctttaaatg ataatcaatt
     8941 gcatgggagt ttatcaccgt gagaaccgta ataacatacc ttcggttcag ttcagctata
     9001 caaggcgctg aaggtgctga ttcaaccaga cgacaaaatg agctattcaa acagtggtta
     9061 aggaagaata gcgatgctca ggtagttgcg tctttcagtg atgaagggtt gagtggttac
     9121 aaaggtaagc accttactgg tcagtttggt gacatgttag cccgtattga ggctggggag
     9181 tttccagcag gtacaatttt gctagttgag agtatcgacc gtataggccg attagagcat
     9241 cttgaaacag aagccttgat gaaccgcatc ttaggcaacg ggatagagat tcacacattg
     9301 caagatggcc ttatctacac aaaggatgct ttagccgatg accttggcat aagcatcatc
     9361 cagcgtgtca aagcctacat agcgcatcag gaatctaagc agaaatcttt ccgtgttagt
     9421 cagaagtggg aacaacgtgc aaaacttgcc cttgctggtg aacaacgtct tacaaagatg
     9481 gttcccggat ggattgaccc tgatactttc aagtgcaatg aacacgctga gactgtaaga
     9541 ctgattttca agctgctgct agatggtgaa agtctgcata acattgcacg tcacctacaa
     9601 agcaacgata taaagtcttt tagtcgccgt aaagatgcta acgggtttag tgttcattct
     9661 gtgcgcacta tcttaaggtc agaaacaacg agaggtacac taccagcatc acagcgcaat
     9721 gaccgccccg ctataccgaa ctactacgaa gccgctatag atgctgcaac gttcaataag
     9781 gctcaagaga ttctcgacaa gaatcgtaaa ggccgcacac ctgcaagtga
//
LOCUS       DAZFVO020000175         9664 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFVO020000175 DAZFVO020000000
VERSION     DAZFVO020000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.176"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(144..356)
                     /gene="cspA"
                     /locus_tag="ACHKNB_RS21215"
                     /old_locus_tag="pgaptmp_004243"
     CDS             complement(144..356)
                     /gene="cspA"
                     /locus_tag="ACHKNB_RS21215"
                     /old_locus_tag="pgaptmp_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HGU4197641.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(637..927)
                     /locus_tag="ACHKNB_RS21220"
                     /old_locus_tag="pgaptmp_004244"
     CDS             complement(637..927)
                     /locus_tag="ACHKNB_RS21220"
                     /old_locus_tag="pgaptmp_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HGU4197642.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1361..2071
                     /locus_tag="ACHKNB_RS21225"
                     /old_locus_tag="pgaptmp_004245"
     CDS             1361..2071
                     /locus_tag="ACHKNB_RS21225"
                     /old_locus_tag="pgaptmp_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HGU4197643.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2121..3095)
                     /gene="ghrB"
                     /locus_tag="ACHKNB_RS21230"
                     /old_locus_tag="pgaptmp_004246"
     CDS             complement(2121..3095)
                     /gene="ghrB"
                     /locus_tag="ACHKNB_RS21230"
                     /old_locus_tag="pgaptmp_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HGU4197644.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3199..3858)
                     /locus_tag="ACHKNB_RS21235"
                     /old_locus_tag="pgaptmp_004247"
     CDS             complement(3199..3858)
                     /locus_tag="ACHKNB_RS21235"
                     /old_locus_tag="pgaptmp_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HGU4197645.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4011..6344
                     /gene="bisC"
                     /locus_tag="ACHKNB_RS21240"
                     /old_locus_tag="pgaptmp_004248"
     CDS             4011..6344
                     /gene="bisC"
                     /locus_tag="ACHKNB_RS21240"
                     /old_locus_tag="pgaptmp_004248"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HGU4197646.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6313..6753)
                     /locus_tag="ACHKNB_RS21245"
                     /old_locus_tag="pgaptmp_004249"
     CDS             complement(6313..6753)
                     /locus_tag="ACHKNB_RS21245"
                     /old_locus_tag="pgaptmp_004249"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HGU4197647.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6750..7313)
                     /gene="tag"
                     /locus_tag="ACHKNB_RS21250"
                     /old_locus_tag="pgaptmp_004250"
     CDS             complement(6750..7313)
                     /gene="tag"
                     /locus_tag="ACHKNB_RS21250"
                     /old_locus_tag="pgaptmp_004250"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGU4197648.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7471..8169
                     /locus_tag="ACHKNB_RS21255"
                     /old_locus_tag="pgaptmp_004251"
     CDS             7471..8169
                     /locus_tag="ACHKNB_RS21255"
                     /old_locus_tag="pgaptmp_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HGU4197649.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8398..9600
                     /locus_tag="ACHKNB_RS21260"
                     /old_locus_tag="pgaptmp_004252"
     CDS             8398..9600
                     /locus_tag="ACHKNB_RS21260"
                     /old_locus_tag="pgaptmp_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4197650.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2280 a         2366 c         2507 g         2511 t
ORIGIN      
        1 gcattctcat tgcacccaaa tttatttttc acaaaaataa tactagattt tattacgcga
       61 tcgattattt atttcctgaa aaacaaataa aaaaatcccc gccaaatggc agggatctta
      121 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      181 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      241 ttctggatag cagagaagtg tacgaacacg tctttagagc catcgtcagg agtgatgaag
      301 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      361 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      421 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      481 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      541 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      601 atcaggaccg taaacagcaa taaagtggat aaaagtctat tccatcaact gcttacttaa
      661 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
      721 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
      781 gatttttaat cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
      841 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
      901 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
      961 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1021 ggcatgctct ggctatgatt cgattcagcc agtcatttag cgtgacttat agccacctaa
     1081 cgatctgata aatgccgata tatctcttac cactcctggt ttgtaaagat ttcgaaaatc
     1141 taaccttctg cgaaggcgta aatctctggc tacaaaaaaa gcaacggcac tatcattttt
     1201 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1261 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1321 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1381 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1441 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1501 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1561 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1621 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1681 aagcggtccg ctgcgtgaaa tgaacggttc gctaggtgtg ctggcgcaac aactgcaaaa
     1741 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     1801 ggtctttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     1861 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     1921 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     1981 aagtgaatat aacaaactga ttgcgccatt accggcacag catcaggcgt ttaatcaggc
     2041 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2101 ttactctacg cagtcgcggc ttagtccgcg acgtgcggat tcacacagtt cttctcaacc
     2161 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2221 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2281 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcccct
     2341 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2401 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2461 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagtgt atccaaatcg
     2521 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2581 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccataccgat ccgtcccatc
     2641 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     2701 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     2761 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     2821 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     2881 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatcaac attctcgttt
     2941 gaacccagta aaccttcagc ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg
     3001 ctgaggtttg ccacctggtg aacggtgaaa tgctcttgca ggcgttgcag taaatcatca
     3061 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3121 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3181 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3241 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3301 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgttg
     3361 ctgggagaga cgcatgttca ggtcgtgacc acccgtgctg tcggtataac caatcacgtt
     3421 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3481 tgccggtttc agggtcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3541 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3601 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3661 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     3721 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     3781 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     3841 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     3901 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     3961 taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact
     4021 catcctcacg atattccgtt ctgactgccg cccactgggg gcccatgctg gttgaaaccg
     4081 acggcgaaac cgtgtttagc tcgcgtggcg cgttagccac aggaatggaa aactccttgc
     4141 agagcgcggt tcgcgaccag gttcacagca atacgcgggt acgatttcca atggtgcgca
     4201 aaggctttct cgcgtcaccg gaaaacccgc aaggcattcg tgggcaggat gaatttgttc
     4261 gcgtgagttg ggatgaggcg ctggatctta ttcaccaaca acataaacgc attcgtgagg
     4321 cttatggtcc ggcatcgatt tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc
     4381 ataaggcctc gacattatta caacgctata tggcgctggc aggcggttat accgggcatc
     4441 tgggggatta ttcgaccggc gcggcacagg cgatcatgcc gtatgtcgtg ggtggtagcg
     4501 aagtttatca acagcagacc agttggccgc tggtgctgga acatagcgat gtcgtggtgc
     4561 tgtggagtgc taatccactc aatacgctga aaattgcgtg gaatgcatcc gatgagcagg
     4621 ggctttctta cttttctgca ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa
     4681 tgcgatcgga aaccgtcgat ttctttggcg ataaaatgga gtgggtggca ccgcacatgg
     4741 gcaccgatgt tgcgctgatg ctggggatcg cccatacgct ggtggaaaat ggttggcacg
     4801 acgaagcgtt tctggcgcgt tgcaccacag gttatgccgt cttcgcctct tatttgctgg
     4861 gcgagagtga cggaatagcg aaaaccgccg aatgggcagc agagatttgt ggtgttggcg
     4921 cagcgaaaat ccgcgagctg gcggctattt tccaccaaaa taccaccatg ctgatggcag
     4981 gctggggaat gcagcgccaa cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg
     5041 cagcaatgtt ggggcaaatc ggcacacccg gcggcggttt tggtctttct taccattttg
     5101 ccaatggtgg taaccccacg cggcgttctg cggtgctctc ttccatgcag ggcagcttgc
     5161 cgggtggctg cgatgcggta gataaaatcc ctgtggcccg cattgttgaa gcactggaaa
     5221 accctggtgg cgcatatcaa cacaacggta tggaccgaca ttttccggat attcgtttta
     5281 tctggtgggc gggcggtgct aactttactc atcatcagga taccaatcgt ctgatccgtg
     5341 cctggcaaaa accggagctg gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac
     5401 acgcggatat cgttctgcct gcgactacct cttttgagcg taatgatctc accatgaccg
     5461 gtgattacag taatcagcat ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag
     5521 cgcgtaatga ttttgatgtt tttgccgagt tgagtgaacg ttgggagaag ggcggctatg
     5581 cgcggtttac ggaaggaaaa agtcagctgc aatggctgga aacgttttat aacgttgccc
     5641 ggcagcgcgg ggcaagccag caggttgaat tgccgccatt tgctgagttc tggcaagcca
     5701 accagttaat tgagatgccg gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt
     5761 tttgccgcga tccgctggcg catccgttaa aaaccgccag cggcaagatt gaaatcttct
     5821 cacagcgtat tgccgattac ggttacccgg attgccctgg gcatccaatg tggctggagc
     5881 cggacgaatg gcagggcaat gccgaaccag aacagttgca ggtactttct gcccatccgg
     5941 cgcaccgcct gcacagccag ctgaattaca gttctctgcg cgaattgtac gcggtggcaa
     6001 atcgtgagcc tgtcaccatt catcctgacg atgcccagga gcgcggcata caagatggcg
     6061 atactgttcg gttgtggaac gcacgcgggc aaattcttgc cggagcggtc attagcgagg
     6121 gaattaaacc tggcgtgatt tgcattcacg aaggggcatg gccggatctg gatttaaccg
     6181 ctgacggtat ttgtaaaaac ggcgcagtga acgtgctgac caaagatctc cccagctcgc
     6241 ggctggggaa tggctgtgcg ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc
     6301 cggaactgac acttacagcg tttgaaccac cggccagctc ataatccatg tgggtagttg
     6361 ggtttcatcc tgccatgcgc aatcgacaat gtgaaaaccc tgtgcctggt aaaaatttat
     6421 cgccggttga tttttttgat aaacctccag catcaggtgg ggatggcgct gctgcacata
     6481 ctgcatcagc gccttaccaa taccgcgcct gacggccttc ggtgcgacaa acatcgctgc
     6541 cagaaatcgg ccttccataa tgctgacaaa accgagaagc ttaccgtctt cttcccagac
     6601 ccagttttgc gcgttggcaa gataggcatc ccgcaccagc ggaatgcagt cacgccagta
     6661 attcgctttt ataaagggat gcccccaggt tgtactttcc agccacagtt cgaggatcgc
     6721 ggggagttct gaacgttgcg cttcccgaat catggtttat ttcccggata gcaacagcag
     6781 ccaaccacat gatcattcac cagcccacat gcctgcataa aggagtaaca gattgtggtg
     6841 ccgacaaact taaaaccacg ttttttcagt gccttagata gggcgtcgga ggcggacgta
     6901 gatgtgggaa tttcgctcaa cgttgtggct tgtgtcacct gtggctgatg atttacaaac
     6961 gaccagacaa agtcgacaaa cggttcgccg ttctgttcca tttgcaggta cgcccgcgca
     7021 ttaccaataa ttgcctgaat tttccctcga tggcggataa tcccggcgtc ctgtaccagt
     7081 ctttcgacat cctcttcctg cattgctgcg accttcaccg gatcgaactg atgaaagcag
     7141 gcgcgatagt tttcgcgttt tttgaggacg gtgatccacg ataacccagc ctgctgccct
     7201 tcaaggcaga tcatttcgaa cagtttttta ctgtcagttt caggcacgcc ccactcatta
     7261 tcatggtagg caatataaag cgggtcctga ctcacccagc cgcaacgttc catactttcc
     7321 ctcgctatgt acccgatcgt aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag
     7381 tcaatattta atacagggct actatgcccg atatccttca tacaatgaca ataatattgc
     7441 cgaattcggc tcttccctgg agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg
     7501 cagctaagcc tgctggcgag cgtggtaatc agtgcctttt ttctcaacac agcttacgcc
     7561 tggcaacaag aatatatcgt tgatacgcaa cccggacatt ccacagagcg ttacacctgg
     7621 gatagtgatc atcaacctga ttacaacgat attttgtcgc aacgtattca aagtagccaa
     7681 agggcgctgg gactggaagt caatctggcg gaagaaactc ctgtggatgt gaccagcagt
     7741 atgagtatgg gctggaattt tcctttatat gaacaggtta caaccggccc ggtcgcggca
     7801 ttacattacg atggcacaac cacctcgatg tataacgagt ttggcgacag tactaccacg
     7861 ctgaccgatc cgttatggca tgccagcgtg agtagcttag gctggcgtgt tgactcccgg
     7921 cttggcgatc tccgaccctg ggcgcaaatc agctataacc agcaatttgg cgagaatatc
     7981 tggaaggcgc aatcaggcct gagccggatg acggcgacaa accagaacgg caactggctg
     8041 gatgtcaccg taggcgctga tatgttgctc aatcaaaata ttgccgccta tgccgcgcta
     8101 tctcaggcag aaaataccac taacaatagc gactatctgt atacgatggg ggttagtgcc
     8161 agattttaac gtaaaaatca caattgaaac cattggataa caacagttgt ggcgatagtg
     8221 ggtgctaact tactaaataa taaatttggt gaataattgt cgcgtcattc attcctgaac
     8281 taaggcattt cattccgttc tgatggcatt tcatgccgtt tttccccagg cataaaatgc
     8341 acttcgttat ggttgtcggc agagattttt cctttttatt actgcaggaa tactgccatg
     8401 acaccttcaa attatcagcg tacccgctgg ctgacactca tcggtactat cattacccag
     8461 tttgcactgg ggtcggttta tacctggagc ctgtttaatg gcgcgctttc tgccaagctg
     8521 gatgcgccgg taagccaggt cgctttctct ttcggcttgt taagtctggg gctggcaatt
     8581 tcgtcttctg ttgcgggcaa attgcaggaa cgttttggcg ttaaacgcgt caccatggct
     8641 tccggcattt tgctgggatt aggcttcttc ctgaccgcgc attccaacaa cctgatgatg
     8701 ctgtggttaa gcgccggtgt gctggtcggt ctggcggatg gcgcgggtta cctgctgacg
     8761 ctctctaact gcgtgaagtg gttcccggag cgtaaaggtc tgatctccgc gttcgctatc
     8821 ggttcttatg gtctgggtag cctgggtttc aaatttatcg acacgcagct gctcgaaacg
     8881 gtgggtttgg aaaaaacatt tgtgatttgg ggagcgattg cgctggtgat gattgttttc
     8941 ggcgcaacgt taatgaaaga cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg
     9001 gagaaagatt acacgctggc agagtcgatg cgtaaaccgc agtactggat gttagcggta
     9061 atgttcctga ctgcctgcat gagcggcctg tacgtgattg gggtagcgaa agatatcgcc
     9121 caaagtctgg cacaccttga tgtggtttcc gcagccaatg cagtaacggt tatttccatc
     9181 gctaaccttt caggtcgtct ggtgctgggg atactgtctg acaaaattgc ccgtatccgt
     9241 gttatcacca ttggtcaggt gatttcgctg gtgggtatgg cggccctgct gtttgcacca
     9301 ttaaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt tggcggcact
     9361 attaccgtct ttccgtcact ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac
     9421 tacggtgtga tttacctcgg tttcggtatc ggtagcattt gtggttcgat tatcgcctca
     9481 ctgtttggcg gcttctatgt gaccttctac gtgattttcg ccctgctgat tctgtcattg
     9541 gcgctttcta cgacaattcg tcagccagag cagaaagtat tgcgtgaggc gcatatttag
     9601 aaatatgttg ctactgaaat aaatgcgagg agggagactt caaagtctcc ctttttttat
     9661 ggac
//
LOCUS       DAZFVO020000176         9593 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFVO020000176 DAZFVO020000000
VERSION     DAZFVO020000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.177"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            114..386
                     /locus_tag="ACHKNB_RS21265"
                     /old_locus_tag="pgaptmp_004253"
     CDS             114..386
                     /locus_tag="ACHKNB_RS21265"
                     /old_locus_tag="pgaptmp_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HGU4197651.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(387..1259)
                     /gene="rluF"
                     /locus_tag="ACHKNB_RS21270"
                     /old_locus_tag="pgaptmp_004254"
     CDS             complement(387..1259)
                     /gene="rluF"
                     /locus_tag="ACHKNB_RS21270"
                     /old_locus_tag="pgaptmp_004254"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HGU4197652.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1471..2160
                     /gene="pepE"
                     /locus_tag="ACHKNB_RS21275"
                     /old_locus_tag="pgaptmp_004255"
     CDS             1471..2160
                     /gene="pepE"
                     /locus_tag="ACHKNB_RS21275"
                     /old_locus_tag="pgaptmp_004255"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HGU4197653.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2251..3882)
                     /locus_tag="ACHKNB_RS21280"
                     /old_locus_tag="pgaptmp_004256"
     CDS             complement(2251..3882)
                     /locus_tag="ACHKNB_RS21280"
                     /old_locus_tag="pgaptmp_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGU4197654.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4102..7785)
                     /gene="metH"
                     /locus_tag="ACHKNB_RS21285"
                     /old_locus_tag="pgaptmp_004257"
     CDS             complement(4102..7785)
                     /gene="metH"
                     /locus_tag="ACHKNB_RS21285"
                     /old_locus_tag="pgaptmp_004257"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HGU4197655.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7985..8809
                     /gene="iclR"
                     /locus_tag="ACHKNB_RS21290"
                     /old_locus_tag="pgaptmp_004258"
     CDS             7985..8809
                     /gene="iclR"
                     /locus_tag="ACHKNB_RS21290"
                     /old_locus_tag="pgaptmp_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HGU4197656.1"
                     /translation="MVAPIPAKRGRKPAVATAPAIGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9126..>9593
                     /locus_tag="ACHKNB_RS21295"
                     /old_locus_tag="pgaptmp_004259"
     CDS             9126..>9593
                     /locus_tag="ACHKNB_RS21295"
                     /old_locus_tag="pgaptmp_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197657.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCR"
BASE COUNT         2315 a         2580 c         2405 g         2293 t
ORIGIN      
        1 cgtcaattat caagaaagat aaatatttaa gaagagaaca aaggaatatt atcagttcag
       61 ctcgcaaacg aattcccgta cactgaactc tctggctcac tgagggcacc atcatggcac
      121 tcccccgtat tacccaaaaa gagatgactg aacgcgagca gcgtgaactt aaaacactgc
      181 tggatcgggc gcggattgcg catggtcgcg tactgaccaa ctccgaaacc aacagcatta
      241 agaaagagta catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc
      301 agttgaagaa aaagcaggct tataaaccgg atcctgaagc gtcatttagc tggtcagcaa
      361 atacgtcgac gcgcggcagg cgctgattag cgccccttct ttttacgccc cggagaggta
      421 aaacgcttac cgttggacgc cgggcgaccg cctttttccg ccgttttttc catcttaacg
      481 actggacgtt tgatgcccgc tgttttcggt ttggccttca ctttaggttt tgcctcggaa
      541 gaggaatttt cgatgagctt aaagaggtcg attaactcat catcagttaa atcgcgccac
      601 tcacccagcg gaatgccgct taaactgacg ttcataatgc gcgtacgttc cagctttttc
      661 acttcgtaac caaagtgctc gcacatgcga cggatctgac ggttcagccc ctgcaccagg
      721 gtaatgcgga agacaaacgg cgcttctttt ttgactttac actttttagt cactgtcccg
      781 aggattggca cccccgcccc cataccacga ataaattcat cggtaatcgg tttatcgacc
      841 gtaaccagat actctttctc atgatcgtta cccgcacgga ggatcttatt caccagatcg
      901 ccgtgattgg tgaggaaaat cagcccctgg gagtctttat ccaggcggcc aatcgggaac
      961 acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc gctcgccatc ttcggtggtg
     1021 ctgacaatac cgacgggctt gttcagggcg ataagtacca aatcttcggc ttcccgaggc
     1081 tcaatcaact gaccgtttac tttcacaacg tcgccgggtt tcacctgatc gccaatggtg
     1141 gctcgcttgc cattaaggaa cacattgcct tgctcgatat agcgatccgc ttcgcggcgt
     1201 gagcaaattc cgctttcgct gatgtattta tttaaacgga ctgatgagtc gggcagcata
     1261 aattctcctg taaaaaacgg aatataccgt acctttgggt tgataaaaaa tagtcatgag
     1321 cgaactattt gttatttcca ttggcttgaa ttcgttacct gctcaaatca ctttccagaa
     1381 gcaatgtgcg acatcgtgag ccatgtcagt aagtggttaa attcgcagtg gtagcatgcc
     1441 ttccgtgatt tcttactaac cggagaagta atggaactgc ttttattgag taactcgacg
     1501 ctgccgggta aagcctggct ggaacatgca ctgccgctaa ttgctgaaca gttgcagggt
     1561 cgccgctcag cggtgtttat ccctttcgct ggcgtaacgc agacctggga tgattacaca
     1621 gcgaaaacgg ctgcggttct cgctccgctg ggtgtttctg tcaccggtat tcatagcgtt
     1681 gtcgatcccg ttgccgcgat tgaaaatgct gagatcgtga ttgtcggggg cgggaatact
     1741 ttccagttgc tgaaacagtg ccgcgagcgc gggctgctgg caccaattac tgacgtggtt
     1801 aaacgtggtg cgctgtatat tggctggagc gcaggcgcta accttgcttg cccaaccatt
     1861 cgtaccacca acgatatgcc gattgtcgat ccgcaaggtt tcgatgcgct aaatctgttc
     1921 ccgctgcaaa tcaacccgca cttcaccaac gcgctaccgg aaggccataa aggtgaaacc
     1981 cgtgagcagc gtattcgcga actgctggtc gtcgcgccag aactgacgat tattggtcta
     2041 ccggaaggta actggatcac agtgagtaaa ggtcacgcta cgctgggtgg cccgaacacc
     2101 acttatgtgt ttaaggctgg tgaagaagcg gtgccgctag aagctggtca ccgtttttaa
     2161 gtaaattgcc ggatgcatga tccggcatct tgttcatagt gcctgatgcg acgcttgacg
     2221 cgtcttatca ggcctacagg attcacaata ttaatactcg tcccgtcctt catcttcatc
     2281 cggctgttcc agcacactgt aagccaccga acaaaacagc gagttcaggc gctgcatatc
     2341 tcccagtaag cctaaatgta gcgaactggt ttcaatgctc tgcacgtttt gctgatgcag
     2401 gcgatcgacg tgggcgtggg aatagcggcg attaagaatg cgaaaacgat gtttgctgcg
     2461 acgcaaacga cgagcgctgg tgacatcgcc agagaagaac accgacattg ccagctttaa
     2521 attactgagc aattgctcat agagcgcatc cagttccttc aacccatcaa gcgaaaatgc
     2581 ccgccgtgct gccagcgatt tatcggcaat ttcgctgccc atgcgctcga cgatatcgga
     2641 agcctgttca aggttgagcg acatttcaat aatctccgcc cagcgacgcg actcttcttc
     2701 tgccagttcc tcttttggca tccgcgccag atacagctta atggcggtat agagaacgtt
     2761 gatatcatct gccagcttac gcagctcttt ctcctgccgt ggctcgccgt gcatcacttt
     2821 attcagccct tccatcatct gttccatggc gtcgccaatg cgcagggttt cgcgcgcggc
     2881 gttcgccaga gcaagcgtgg gcgtatccag cgcgctgaca tccagatgtt taggccgtag
     2941 ctgggtatcc agttccggtt catcgcgaat aatcgttttg caaaaccgtg ccatcgggtc
     3001 aacaaatggc agcatgacca ggcaacgtac aaggttgtag aagacgtgga aatagatcac
     3061 cagttccgct tttggcaatg acagcttccc cattgtctct gccagcaaat ggacaaacgg
     3121 caggataatc aggctaccca ccagcttaaa cagcagacta cccagcgcga cacggcgggc
     3181 tgcggcattg gcggcactgt tgttgagcat cgccagcagg ccggaaccga ggttagcacc
     3241 aatcaccaga cagagcgcca cggggaagga gataatgcct gcggcggtca gagtcgcagt
     3301 cagcagtaca gcagcaaggc tggagtagct gataatggcg aacatcgcgc caatcagcgc
     3361 atccagcaga atatcgccgg tcagcgaggc aaagatcacc tgaacgccgt ttgcctgggt
     3421 gatcggcgtt acggcctgca caatcaactc cagcgccagt aaaatcagcc caagaccaat
     3481 accgacgcgg cccagttgcc cggcgcgtga ctgtttgcgt ccgaggaaaa aaatcacgcc
     3541 gataaaaata agtaacggtg acagccagga taagtcgaag gtgagaatac gcgccattag
     3601 cgccgtcccg acatctgccc ccagcacaat gaccagagcc ggtgcgaggg ctaccagatc
     3661 ctgagcgaca aacgaggtca ccagcatggt ggtggcatta ctgctctgca ccagtgcggt
     3721 aacgccgatc cccgcgcaaa aggcgagcgg ctttttttcg acgctccggc taaggacagt
     3781 acgcaaacgc gcgccgaaga cgcgcattac gccggttcga acaatatgag tcccccagac
     3841 cagcagggcg acggcagaaa gcaggtgaag cagcgttaac acgagttcag gttctcctta
     3901 tcgttatatg ttcctgaagc aattgaatga ccaaaggacc ggaaacacgc tgactggcat
     3961 ctttactgtg ccttttgaca ttcgtttttc cggctaagat tattccagcc tgtttccagt
     4021 ataagtgttt catattaata aagagacagg gcactcattg agcgccctgt ttgttgtaag
     4081 gaaaagtgac agatttgtga atcagtccgc gtcatacccc agattcggtg ccagccagcg
     4141 ctcaacatcg gagacgctca tacctttacg gcgggcatag tcttcaacct gatcgcgctg
     4201 aatttgcgcc accgcgtagt acttgctgtc cgggtggctg aaataccaac ccgaaaccga
     4261 tgcaccgggc cacatggcga aagattctgt gagtttcatg ccggtatgtt tttccacttc
     4321 cagcagctcc cagatggtgg ctttttccgt atgttccggg caggccgggt agcccggtgc
     4381 cggacggatg ccctggtagt tttcgcggat cagttcttcg ttgctgaggt tctcgttcgg
     4441 cgcatagccc cagtagactt tacgcacacg ctcatggaga tactccgcaa aggcttcggc
     4501 taaacggtcg gcaagcgctt tcaccatgat tttgttgtaa tcatcgtgct gcgcttcaaa
     4561 ggcatcagcc agtgcgtcct cttccagccc gccagtcacg gcaaatgcgc cgatgtaatc
     4621 tgctttacca gaaagcttcg gcgcaacgaa gtcagcgaga cagtagttag cgaatcccgt
     4681 tttttcggtc tgttgacgca gatggtggct gacgttgatc acatgggtgc gcgtttcgtc
     4741 acggtagatt tcaatgtcat cgcccacacg gtttgccggg aacaggccca ccacgccacg
     4801 cggattcagc gttttctcgg cgcttaattt atccagcatg tcgttggcgt ctttaaacag
     4861 ccgctgcgcc tcaacgccca ccacttcatc ttccagaatg cgcggatact tcccggccag
     4921 cgaccaggtc ataaagaacg gtgtccagtc gatgtaatta cgcagagttt cgatgctggc
     4981 ttcaacttcc tgcacgccga gacggtgcgc caccggcggc gtgtaagcct gccagtcaaa
     5041 agcgaaatcg ttatcgcgcg ccgcttccag cgtgaccggt ggtgtgcgcg gtttcttacg
     5101 cccgtgctga atacgtacgg tttcgtactc cttgcgggta cgagcgacaa aatcatcacg
     5161 ctgagtatcg gaaagcagcg ccgccaccac accaacggtg cgcgatgcgt tctgtacata
     5221 caccgtcggg ccgctgtagt tctgctcgat tttcaccgcc gtgtgcgctt ttgaggtcgt
     5281 cgcgccgcca atcaatagcg ggatggtgaa gccctgacgc tccatctctt tcgccacgtt
     5341 aaccatctcg tccagcgacg gcgtgataag ccccgaaagt ccaatcagat cagcattcac
     5401 ttctttagcg gtacggagaa ttttttccgc aggcaccata acgccgagat cgacaatttc
     5461 gtagttgtta cattgcagca ccacaccaac gatattttta ccgatgtcgt ggacgtcgcc
     5521 cttcacggtg gcgatcacca tcttgccgtt ggttttgccc tgctctttgc tggcttcaat
     5581 aaacggttcg agatacgcca ccgcctgttt catgacgcgc gccgatttga ccacctgtgg
     5641 caggaacatt ttcccttcgc caaacagatc gccgaccacg ttcatgccgt ccatcaacgg
     5701 gccttcaatc acttcaatcg ggcgcgtagc ctgctggcgg gcttcttcgg tatcctgctc
     5761 gataaactcg gtaatgcctt tgaccagcga gtattccaga cgtttattca cttcccacga
     5821 gcgccactcc gcctgctggg cgttagcggt gtcgtcggtt ttgctgccgc gatatttctc
     5881 ggcaagctcc agtaaacgct cagtgccatc atcgcgacga ttaagaatca catcttccac
     5941 cgcgtcgcgc agttcagcgg gcaggtcgtc gtaaatcgcc agctgcccgg cattgacgat
     6001 ccccatatcc atgccattgc gaatagcgta gtagaggaac actgcgtgaa tggcttcgcg
     6061 caccggatcg ttgccacgga acgagaaaga aacgttagat acgccgccgg aaattagcgc
     6121 gtgcggcagt tcgcgtttga tgtcttcaca cgcgccgata aagtcctgcg cgtagttgtt
     6181 gtgctcttca atgccggtgg caaccgcgaa gatgttcggg tcgaagatga tatcttctgg
     6241 cgggaagcca acctcttcgg tgaggatttt gtacgcccga cggcaaatct cgattttccg
     6301 tgcgcgggta tcggcctgtc cctgttcgtc aaaggccatt accaccactg ccgcaccgta
     6361 acgacgcaag agcttcgcgt gatggataaa ggcgtcgacg ccctctttca tcgagataga
     6421 gttaacaatg cctttgccct ggatacactt cagacctttt tcaatgacat cccattttga
     6481 ggagtcgatc ataatcggca cgcgagcgat atccggttca ccggcaatca gattgagaaa
     6541 acgcaccatc gccgcttcgg cgtcgagcat cccttcatcc atgttgatat cgataatctg
     6601 cgcaccgttt tccacctgct ggcgggccac atccagtgcc tcgctgtatt tctcttcttt
     6661 gatcaggcgc ttgaacttag cggaaccggt gacgttggtg cgttcaccca cgttcacaaa
     6721 caggctatct tcgccaatgt tcagcggctc caggccggac aaacggcagg ctacggggat
     6781 ttccggcagt ttgcgaggcg ctaatccttc tactgcacga ctcatcgctg caatatgttg
     6841 tggcgtggtg ccacagcagc cgccgacgat attgagaaaa cccgcttgcg cccattcacg
     6901 tatctgtttt gccatcgtgt cggcgtcgag atcgtactca ccaaaggcgt tgggtagccc
     6961 ggcgttcggg tgcgcggtga cgtagcattc cgcaatccgt gacagctcct gcacgtactg
     7021 gcgcagttca tcgggcccca gcgcacagtt caggccaaag gtcagagctt cggcgtggcg
     7081 caatgagttg taaaatgctt cggtggtctg cccggagagc gtgcgcccgg aggcgtcggt
     7141 gatggtgccg gagatcataa tcggcagctc aacgcccagc gcttcaaact ccgttttcac
     7201 cgcaaaaacc gccgctttgg cgttaagggt gtcgaaaacg gtttcaatca ggatcagatc
     7261 cgcaccacct tccaccagcg ctttggtgga ttcgcgatag gccgccacca gctggtcaaa
     7321 agtgatgttg cggaatgccg gatcgttgac gtccggagaa atagacgccg tgcggttggt
     7381 tgggccgaga acaccggcaa cgtagcgcgg tttctccggc gtgcgcgcgg tccactcgtc
     7441 agcacaggcg cgcgccagtt tcgccgccgc aaagttgatt tccgccgaca gggattccat
     7501 ctggtaatcc gccatcgcaa tggtcgtgga gttgaaggtg ttggtttcga tgatatccgc
     7561 gcccgcttca aagtaggcgt tgtggatagc ggcgatcact tccggtttgc tgagcaccag
     7621 caggtcgttg ttgcctttga gatcgcatgg ccagtcggca aagcgttcac cacgaaaatc
     7681 ggcttcgttc agtcgatagc tctggatcat ggtgcccata ccgccgtcca gcaccagaat
     7741 acgttcattt aactgcgcac gcagttgttc cactttgctg ctcacacttg ctcccgacac
     7801 gctcaaccca gatttaataa aaattcaaca aaccatactg gcataaacgc atctgtggta
     7861 aaagcgacca ccacgcaaca tgagatttgt tcaacattaa ctcatcggat cagttcagca
     7921 actattgcat tagctaacaa taaaaatgaa aatgatttcc acgatacaga aaaaggaggc
     7981 tgtcatggtc gcacccattc ccgcgaaacg cggcagaaaa cccgccgttg ccaccgcacc
     8041 agcgattgga caggttcagt ctttaacgcg tggcctgaaa ttactggagt ggattgccga
     8101 atccaatggc agtgtggcac tcacagagct ggcgcaacaa gccgggttac ccaattccac
     8161 gacccaccgc ctgctaacca cgatgcaaca gcagggtttc gtgcgtcagg tcggcgaact
     8221 gggacattgg gcaatcggcg cacatgcctt tatggtcggc agcagctttc tccagagccg
     8281 taatttgtta gcgattgttc accctatcct gcgcaattta atggaagagt ctggcgaaac
     8341 ggtcaatatg gcggtgcttg atcaaagcga tcacgaagcg attattatcg accaggtaca
     8401 gtgtacgcat ctgatgcgaa tgtccgcgcc tatcggcggt aaattgccga tgcacgcttc
     8461 cggtgcgggt aaagcctttt tagcccaact gagcgaagaa caggtgacga agctgctgca
     8521 ccgcaaaggg ttacatgcct atacccacgc aacgctggtg tctcctgtgc atttaaaaga
     8581 agatctcgcc caaacgcgca aacggggtta ttcatttgac gatgaggaac atgcactggg
     8641 gctacgttgc cttgcagcgt gtattttcga tgagcaccgc gaaccgtttg ccgcaatttc
     8701 aatttccgga ccgatttcac gtattaccga tgaccgcgtg accgagtttg gcgcgatggt
     8761 gattaaagcg gcgaaggaag tgacgctggc gtacggtgga atgcgctgac tttttctggc
     8821 gggcagaggc aatattctgc ccatcatacc tgagtggcaa tagaataagg gtgtctgtta
     8881 atcgcattga cgccaaaata acttaatgtc atacacttca cacactttta ttaactcagc
     8941 attattttta aacatcaaac tacttaatca ttaacaaaat cgcaagatct cattccaccg
     9001 aaacttttac ggctgcatta tgagattgag cgatttatga tatttcttta gttgtaatca
     9061 tattaaattt tttcactgtt ctgatagtta aaattcaaga catcaataaa caatgagata
     9121 tttaaatgat tactcgtatc cctcgtagtt ctttctctgc aaatattaat aatacagccc
     9181 agacaaatga acaccaaacc ctgagtgaat tgttttataa agaactcgag gataaatttt
     9241 ctggcaagga gctggcgacg cctctattaa aaagcttctc agagaactgt agacaaaatg
     9301 gtcgtcatat ctttagcaac aaggattttg tcattaaatt ttccacgtcc gtcttacaag
     9361 ctgataagaa agaaattacg ataattaata aaaacgaaaa cacgacactt actcaaacca
     9421 ttgccccaat atttgaagaa tacctaatgg aaattttacc tcaacgctca gacactcttg
     9481 ataaacaaga attaaaccta aaatcagata gaaaagaaaa agaattccca agaattaaac
     9541 ttaatggtca atgttatttt ccggggcgac cccaaaaccg tatagtatgc cga
//
LOCUS       DAZFVO020000177         9545 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFVO020000177 DAZFVO020000000
VERSION     DAZFVO020000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.178"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHKNB_RS21300"
                     /old_locus_tag="pgaptmp_004260"
     CDS             complement(112..1599)
                     /gene="yeeO"
                     /locus_tag="ACHKNB_RS21300"
                     /old_locus_tag="pgaptmp_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HGU4197658.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1760..1835
                     /locus_tag="ACHKNB_RS21305"
                     /old_locus_tag="pgaptmp_004261"
     tRNA            1760..1835
                     /locus_tag="ACHKNB_RS21305"
                     /old_locus_tag="pgaptmp_004261"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1793..1795,aa:Asn,seq:gtt)
     gene            complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHKNB_RS21310"
                     /old_locus_tag="pgaptmp_004262"
     CDS             complement(1873..2823)
                     /gene="cbl"
                     /locus_tag="ACHKNB_RS21310"
                     /old_locus_tag="pgaptmp_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HGU4197659.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHKNB_RS21315"
                     /old_locus_tag="pgaptmp_004263"
     CDS             complement(2925..3842)
                     /gene="nac"
                     /locus_tag="ACHKNB_RS21315"
                     /old_locus_tag="pgaptmp_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HGU4197660.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRKKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4169..4244
                     /locus_tag="ACHKNB_RS21320"
                     /old_locus_tag="pgaptmp_004264"
     tRNA            4169..4244
                     /locus_tag="ACHKNB_RS21320"
                     /old_locus_tag="pgaptmp_004264"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4202..4204,aa:Asn,seq:gtt)
     gene            complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHKNB_RS21325"
                     /old_locus_tag="pgaptmp_004265"
     CDS             complement(4300..5235)
                     /gene="ldtA"
                     /locus_tag="ACHKNB_RS21325"
                     /old_locus_tag="pgaptmp_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HGU4197661.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5297..6376)
                     /gene="cobT"
                     /locus_tag="ACHKNB_RS21330"
                     /old_locus_tag="pgaptmp_004266"
     CDS             complement(5297..6376)
                     /gene="cobT"
                     /locus_tag="ACHKNB_RS21330"
                     /old_locus_tag="pgaptmp_004266"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HGU4197662.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMLGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6388..7131)
                     /gene="cobS"
                     /locus_tag="ACHKNB_RS21335"
                     /old_locus_tag="pgaptmp_004267"
     CDS             complement(6388..7131)
                     /gene="cobS"
                     /locus_tag="ACHKNB_RS21335"
                     /old_locus_tag="pgaptmp_004267"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HGU4197663.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLVKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7128..7673)
                     /gene="cobU"
                     /locus_tag="ACHKNB_RS21340"
                     /old_locus_tag="pgaptmp_004268"
     CDS             complement(7128..7673)
                     /gene="cobU"
                     /locus_tag="ACHKNB_RS21340"
                     /old_locus_tag="pgaptmp_004268"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HGU4197664.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHETVLLECVTTMVTNLLFDYGGDKDPGE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7807..8166)
                     /locus_tag="ACHKNB_RS21345"
                     /old_locus_tag="pgaptmp_004269"
     CDS             complement(7807..8166)
                     /locus_tag="ACHKNB_RS21345"
                     /old_locus_tag="pgaptmp_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HGU4197665.1"
                     /translation="MHCRGCGFIGEEWQQDFQPLDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWFPHSEGTTVILIAEHNARLLTGPAGGAVALIHYVNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            8145..8441
                     /locus_tag="ACHKNB_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /pseudo
     CDS             8145..8441
                     /locus_tag="ACHKNB_RS21350"
                     /old_locus_tag="pgaptmp_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(8542..8871)
                     /gene="tmaR"
                     /locus_tag="ACHKNB_RS21355"
                     /old_locus_tag="pgaptmp_004271"
     CDS             complement(8542..8871)
                     /gene="tmaR"
                     /locus_tag="ACHKNB_RS21355"
                     /old_locus_tag="pgaptmp_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HGU4197666.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9043..>9545)
                     /locus_tag="ACHKNB_RS21360"
                     /old_locus_tag="pgaptmp_004272"
     CDS             complement(9043..>9545)
                     /locus_tag="ACHKNB_RS21360"
                     /old_locus_tag="pgaptmp_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197667.1"
                     /translation="SAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKESRIPKSI
                     YEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYE
                     GNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLI
                     CRALRK"
BASE COUNT         2590 a         2544 c         2100 g         2311 t
ORIGIN      
        1 actacagagg aatcgtgaga acgaggcgaa tattagcgat gcccacccat aatgtcaaag
       61 cctgtttttt aaatttgaaa tcgtttgctg aaataatctg cattttgtcg tttattccga
      121 cacagctggc tttttttcac acttttgcgg ttcggatcga gggtatttcc acagccaacg
      181 tccagtaacc attcgccagt aaaacagcac ggcccgcaca gcccagtcgg caaacattcc
      241 catccagaca ccaaccacac cccagccaag catgatgccc agcacataac cgacgacaac
      301 ccggcaaccc cacatgctca acatagaaac ccacatggcg taacgggcat cgcgagcacc
      361 tttaaatcca gcgggtagca cccatgaggc ggaccaaata ggcataaata aagcatttag
      421 ccaaatcaga atcacaacga catgtttaac ctgtggatcc tgggtgtaaa acgatgccat
      481 aaccccggca aagggagccg ttagccaggc gatggccgtt aatccaagag tggaaagcca
      541 gaacacatgc cgcaactgaa tctctgcttg cgctatctgc cctaccccca accttcggcc
      601 tgtaatgatc gtagaagcag agccgagcgc acttccgggt aagttgataa gagccgcaat
      661 tgaaaacgcg ataaaatttc cggcaataac actggtcccc atcccggcaa cgaacatttg
      721 ggttaataac cgaccactgg taaataacac tgattcgaca ctcgctggaa taccaatccc
      781 catgacttcc cagataatgc taaaattcag cggtttaaaa tagctcttta acgaaatcct
      841 tagcgcagga ttaaaaccaa tcgccagcac ccacaaaatt gcaactgcgc caatataacg
      901 agaaatggtt aaacccaacc cagccccgac aaatcccagt cccggccagg agaaaaggcc
      961 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     1021 cggtattttc gtattccctg caccacgaag tgccccgcta ccaatcaaag tgatggcagc
     1081 tgctggataa ctgagtaccg tcagctccag ataagtcaac gccagtgctt taacttctgt
     1141 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa taagcgttgc
     1201 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     1261 tcgtcgatcc cgcttaccga gactaaatgc cacaacgaca gtagtaccaa gatcgatagc
     1321 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aatcccacgc cggccatcgc
     1381 atcttttccc agccagctga ccagaaaagt gctcaggaca cccatcaaca ggacacaggc
     1441 attctccatg aagataggaa cagcaagcgg ggttatctcg cgccagaaca acactttgta
     1501 gctcttgcgt ttagcgtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     1561 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaataatg agggagaatc
     1621 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     1681 aaacggcaaa cagttgggga atttaaaaat cgggtttgac aaaagatttt tcgccgttaa
     1741 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     1801 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     1861 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctgccg
     1921 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc agacatagtt
     1981 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     2041 cgtatccaga cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     2101 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gcgcacttaa
     2161 tacaatatct gccagcaaac ctttgcgggc aaatgcgtca tcaatacgtg agcgccccgt
     2221 aatcccccgt cggtaagtga ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     2281 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac gaaaccacgg
     2341 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     2401 gccattttgc aacaacgtcg caatttcctg tggcgtcccc tggattagct cgagccgaac
     2461 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     2521 atgcgtcgtt gcaatagtga gaacgccaga cgtatcgttg gtaaacaggt ctgcaagccg
     2581 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     2641 ttcagtcatg cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caagttcatc
     2701 ctccagttcc cgaatatgac ggctgacgcc tgactgtgag gtaaaaagca tattcgcaac
     2761 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     2821 cacggtaaac tccgggcagt tcagatttcc cgttattgtt aaagtctaat gcccggcata
     2881 acaaataata aaaacccgca tcttattcca ttccgatata acacttagct caccaattgc
     2941 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     3001 tgtgcctgtg gcgataagtt cgctctggcg ggtaaattta atgacaaaga gagactcatg
     3061 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgtgcggcc
     3121 gattcgggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     3181 gactcaattt caccaataac ttttgccgtg agtcgccgta gggaaaaagc ctcatcaaca
     3241 cgaagtctaa tagcactgta atcactgggg agaaagaggt tcatttgcgc aatagcattc
     3301 acatcaacgc tttgccccgg acaatcttga gttcctacca ggaaaagatc ttctttcagc
     3361 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     3421 tggtgattta tcagtttttc gttaagcact gcaccgctat tttcatgaag atagataacg
     3481 atctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     3541 gttcctggcg caaagccaat cgagacttgc cccgataatg cctgaccaac gttatgcacc
     3601 gccagttggg cctgctcaca ctgacgtaaa atggcccgcg catgggtata gagaattttt
     3661 ccggcgtctg ttggcgtaac gccccgtttt ttgcgaatca aaagttgttg atttaactca
     3721 ccttccagtg tggcaacctg ctggctgagc gctggttgtg caatatgcaa tacttcagca
     3781 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     3841 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagactgc
     3901 aagatgcttg ccagttttat tttggcgttg atgtacaagc taaccaactg tcaaataaga
     3961 gattatgata gattcgtcat ttgctccttt aatcagctgt cgcgttcccc tgccctataa
     4021 aaggagggta tgcaccacga tgattcatta cccaataaga ttgaaagctc accactttgt
     4081 tgaaattgac agcaaacaaa caaaaaaatg catttcaccc tttgacatca ccatgcactg
     4141 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     4201 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     4261 ctttctagca ggctttttgc tttctaatta ccaacgctct taaaacatct gtcttgaacc
     4321 agaactaatt tgcacaggca ttcccgatcg acgttgcaac gcagcatttg cgcgatttac
     4381 atcaacttct tgcccgctga taaacgcccg caaagatggg gttaccggca atggcacttt
     4441 tcggtcagac tcatattctg cacgattgcg cgacaacggc tcatgaactt ccagccagtt
     4501 cgagccatct ggttcagtgg tgtattttac tggctggtcg ataatttgca cacgcgtccc
     4561 aacaggaaca ttatcaaaca gatatttgat atcgtcattg cgcagacgaa tacagccctg
     4621 acttacccgg agcccaatac caaaattggc attggtacca tggatggcat acaacctgcc
     4681 aatataaatc gcgtacagcc ccatgggatt atcggggccc gcaggaacaa atgcgggcaa
     4741 actctcccct cgtttcgcat attcgcgccg agtgttcggc gttggcgtcc aggttggagc
     4801 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     4861 gataccaata ggaaagactt ccacagtatt actgtctggt gggtagtaat aaagacgcat
     4921 ctcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     4981 aatggtgagt tgcgagccag acttcggcaa aaaaacatca gcgcccgggt tcgcttccag
     5041 catgttactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gggtattgtg
     5101 atcaggaaca gttacagtaa acgactgccc cactaaacgg ctaccctctg gaggtaatgg
     5161 ataagttacc gccaggctag tatggctggc aaaaagtaga gcaaatgagc aaaaaatatt
     5221 tacacgacgc atcatgtccc tttcctaagt cgcgaaagct atccgttaat tatagctttt
     5281 atcagacttt tcgtttttaa ctgttcaaat cagaagtcgt attccccggt agaacaatat
     5341 tactggcagc aagttcgccc atgttgttat atatcgcaca ggcagcttcg atgatgggca
     5401 tcgccagagc tgcgccactc ccctcaccta aacgcatctc catattgaga taaggctcca
     5461 gccccaaatg cgacagcgca atacgcgccc ctttttctgc cgacaggtga gaaggaatga
     5521 gatacggttt gattgcagga gacatctggc aggctgcgag cgcggcagca taagaaagaa
     5581 atccatccag caacacgggt aaaccgcagg aagcagcgcc taacatcacg ccagccattc
     5641 cgaccaaatc aaatccaccc acttttgcca ggacatcaac gccatcctgc ggatttggtt
     5701 gattcaacgt aatcgcccgg cgcacaacat caattttatt agccagttta tctgtcggca
     5761 ggtttgcgcc aatcccaacc acttcttcag gagcccggcc agtgattgtg ctgactattg
     5821 ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc gtaacaccgt
     5881 tttttgccag ctcccgcgta taacatatga cgtccaaaag caacttttct gcctgatggc
     5941 gactcattgc cggagctgaa gcaatattgc cactacctcg agcaacacgc atgttgataa
     6001 gcccggggat aggctcagca gtatcaatac caacatcaat tacgtagacg ttagcgcccg
     6061 cttgtgctgc cagcacacac acgccggttg ttccgcgggt catattttca gcctgaatgg
     6121 ctgtcacttc ttttggggaa atagcgaccc cttcctccca gacgccgtga tcggcacaca
     6181 tgaccagtac cgcttttttg cccacatgcg gaacgccatt caaccccagc attcctgcca
     6241 gttgtatggc aagcgcctcc agccttccca ggctaccaac aggtttgagt aacccgtcga
     6301 tatgccgttg tgcacgcgac atagcggcag gatcgatagc agggatcgta ttcagtaaat
     6361 cggcaagtgt ttgcatgtca cgtctcttta tagcagtgcc agcaggaaga ccagttcacc
     6421 aagctctata gcagctccca gcgtatcgcc ggtttgcccg cccagcgtac gtttgagaag
     6481 ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc
     6541 cggcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc
     6601 atcaattttg ccaatgaaca cattgccaag cccttcttcc cgggcataac gatgacgata
     6661 catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat
     6721 cggttcgcca cgcagagcca actcgcttaa caccagaatc tttaccagta caacaaaaat
     6781 caatgccaga ccgccgtggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc
     6841 gcggctacgt gcagaaaata cgccatcaca ggtatcggcg aggccgtcca ggtggaatcc
     6901 cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggta cgccacacca
     6961 tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc caattaaagg
     7021 aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc agcgacgcgg
     7081 tacaggcaga cgcgtaataa aagagagcat cgcccaaaat aatttactca tttgattttt
     7141 actcctatac ccgaaaccac cagccatact tcatttgccg cagcggccag ctgctgattt
     7201 acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt
     7261 cccacttcgt tagtcactaa tacaaccttt gcgggacaac gttggcaggc agcaatcaac
     7321 gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc accagggtct
     7381 ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaat
     7441 acagtttcat gcgggttaat gtctgcatga attaattcat caagatgttg ccaacgctcc
     7501 accgtgcgcc agtgcgccgg gcggccttgc cgatgatgtt ctatccgtgc agccatctca
     7561 tcatcaagga tttgtgaggt ggcgatatac agaacctgtg aagagtcccc aataagcgcc
     7621 tctgcgtggc gactcttccc gctccgtgcg ccccccgtca ccaaaatcat cataccgact
     7681 cctgattcaa ttgtatgatg gtattcattg aacagaacaa tggatccccc accgttgctg
     7741 accgcaaaac atttacgcga ttaatactgc gcgtaatata attaaaaatc cagcattctc
     7801 aatccattac gaaagaagat ggcattgcgg aatagccaga cttcctcgtg ttaacgtccg
     7861 acaaaatgcg tcattgacag cggtttctct ggaatgacag atttcattca cataatggat
     7921 taatgctaca gccccaccag ccggaccagt cagcaaacgg gcattgtgtt ctgcaatcaa
     7981 aattaccgtc gttccctcag aatgagggaa ccattctggt acggatatct cgctttcagc
     8041 tgctatcaat agcagcactg aataatcgct atacgcatct gataacgatt gcacaggccc
     8101 accatcaaga ggctggaaat cttgctgcca ctcctcaccg ataaaaccgc agcctctaca
     8161 gtgcattgtt ggtcagtgcc tgcgaagcag acattatcgc gctggtgctt aatgcggacg
     8221 caccgtggtc acctttttcg ccaggcttta cctcgtcaat gaatcgcccg gtcattggag
     8281 tagtcaccaa agcagattta gccagcatgg aacaaatatc tttggtgaaa tgttggttac
     8341 gtgaagctgg agcgcacaac gtgttagtaa ccagtgcggt taataacaat ggagttgcag
     8401 aactttttgc cttgctgcat acagaagaag gctgtcgcta atttcgacag gttctcagat
     8461 gagaatgatg acggtgatac acagaaaaga atcaatgagc aatgagggtt gccaggcaac
     8521 cctcattgaa taaaacagga attacttcgc ttcgccgttt ttcatttcgc ccatagcttt
     8581 cagctttttg gagatatcgc ggcgttcttt ggagagctcg gcatttttga tgatgtaatc
     8641 gtcaacgcga tcttcatagt cacctttcat gctggcgatg atgccctgga ttgcttcaac
     8701 gctcatcccc ggcttgatgt aatcgctcag gttgtccagc agcaggacgc gcttctggtt
     8761 gtcacggatc tttttctcaa cgtcctgaat ttcacgttgc agtttgttct tacgacggaa
     8821 cagacgaaca aattccagta cgtcctggaa tgaaggcttg gtagtttcca tttttatacc
     8881 cctgataatg tgagagtcgg attcgtttaa gcaaccgcta ttcgttaggg ccaacattac
     8941 tcatagctgc cgcagatgac aatgctttta tcccttttca ctatcatacc ctttatcctt
     9001 gctgaatcga agcagcagca agatgattct gaagttcagg aattatttgc gcaaggcccg
     9061 gcaaatcaga ttcgatagca actcaagctg atgtgccgtt tccatgttca gccacacata
     9121 accatagatt ggtgtttcca caaccttcag gtcatggtgg ttattgagca actgacgcag
     9181 ctcttccaca gcatcgttca atttttccgt attggcaaaa accggctgcg gattaccttc
     9241 gtacagcgca tgaacaaggc tcagcagtat ttgctgcatc atgtgctggg tatcacgaag
     9301 tttttgcgcg ttcaataaca cgaaatggct ggggcgcgtg gcccagtacg cattgatttg
     9361 caactccagc atacaaacca gattgcgatt aatggtctga ataccttcat atatcgattt
     9421 tggaatacga ctttctttgc tggcgggcgc aatcagtcca cgcattttca cggcatcggt
     9481 caggagtttt tgtagatggc tttccagacg tgggcgttcg agtaagttcg gtgagaatgc
     9541 agatt
//
LOCUS       DAZFVO020000178         9496 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFVO020000178 DAZFVO020000000
VERSION     DAZFVO020000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.179"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1797
                     /locus_tag="ACHKNB_RS21365"
                     /old_locus_tag="pgaptmp_004273"
     CDS             <1..1797
                     /locus_tag="ACHKNB_RS21365"
                     /old_locus_tag="pgaptmp_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HGU4197668.1"
                     /translation="SAYQEGRWETLPFQRAIMNAMGSDYVREVNVVKSARVGYSKMLL
                     GVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTL
                     TMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEG
                     SVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLK
                     WTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPP
                     DSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEV
                     MAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDE
                     QTLQRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGAS
                     VYGKPVANMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDI
                     FDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDL
                     SALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            1794..2000
                     /locus_tag="ACHKNB_RS21370"
                     /old_locus_tag="pgaptmp_004274"
     CDS             1794..2000
                     /locus_tag="ACHKNB_RS21370"
                     /old_locus_tag="pgaptmp_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HGU4197669.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            1997..3598
                     /locus_tag="ACHKNB_RS21375"
                     /old_locus_tag="pgaptmp_004275"
     CDS             1997..3598
                     /locus_tag="ACHKNB_RS21375"
                     /old_locus_tag="pgaptmp_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4197670.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3579..4898
                     /locus_tag="ACHKNB_RS21380"
                     /old_locus_tag="pgaptmp_004276"
     CDS             3579..4898
                     /locus_tag="ACHKNB_RS21380"
                     /old_locus_tag="pgaptmp_004276"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HGU4197671.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQVT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            4908..5240
                     /locus_tag="ACHKNB_RS21385"
                     /old_locus_tag="pgaptmp_004277"
     CDS             4908..5240
                     /locus_tag="ACHKNB_RS21385"
                     /old_locus_tag="pgaptmp_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HGU4197672.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTYYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5296..6321
                     /locus_tag="ACHKNB_RS21390"
                     /old_locus_tag="pgaptmp_004278"
     CDS             5296..6321
                     /locus_tag="ACHKNB_RS21390"
                     /old_locus_tag="pgaptmp_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HGU4197673.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            6363..6758
                     /locus_tag="ACHKNB_RS21395"
                     /old_locus_tag="pgaptmp_004279"
     CDS             6363..6758
                     /locus_tag="ACHKNB_RS21395"
                     /old_locus_tag="pgaptmp_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HGU4197674.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTEHGLARMQ"
     gene            6770..7123
                     /locus_tag="ACHKNB_RS21400"
                     /old_locus_tag="pgaptmp_004280"
     CDS             6770..7123
                     /locus_tag="ACHKNB_RS21400"
                     /old_locus_tag="pgaptmp_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HGU4197675.1"
                     /translation="MADSDNLFDAAIARADETIRGYMGTSATMTSGELSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7135..7713
                     /locus_tag="ACHKNB_RS21405"
                     /old_locus_tag="pgaptmp_004281"
     CDS             7135..7713
                     /locus_tag="ACHKNB_RS21405"
                     /old_locus_tag="pgaptmp_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4197676.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            7710..8105
                     /locus_tag="ACHKNB_RS21410"
                     /old_locus_tag="pgaptmp_004282"
     CDS             7710..8105
                     /locus_tag="ACHKNB_RS21410"
                     /old_locus_tag="pgaptmp_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HGU4197677.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8113..8853
                     /locus_tag="ACHKNB_RS21415"
                     /old_locus_tag="pgaptmp_004283"
     CDS             8113..8853
                     /locus_tag="ACHKNB_RS21415"
                     /old_locus_tag="pgaptmp_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4197678.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            8869..9291
                     /locus_tag="ACHKNB_RS21420"
                     /old_locus_tag="pgaptmp_004284"
     CDS             8869..9291
                     /locus_tag="ACHKNB_RS21420"
                     /old_locus_tag="pgaptmp_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HGU4197679.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            9318..>9496
                     /locus_tag="ACHKNB_RS21425"
                     /old_locus_tag="pgaptmp_004285"
     CDS             9318..>9496
                     /locus_tag="ACHKNB_RS21425"
                     /old_locus_tag="pgaptmp_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4197680.1"
                     /translation="MGRPDWRAMLAGMSSTEYADWHRFYSTHYFHDVLLDMHFSGLTY
                     TVLSLFFSDPDMHPL"
BASE COUNT         2139 a         2480 c         2932 g         1945 t
ORIGIN      
        1 tccgcatacc aggaagggcg ctgggaaaca ctgccctttc agcgggccat catgaatgcg
       61 atgggcagtg actacgtccg cgaggtgaat gtggtgaagt ctgcccgtgt cggttattcc
      121 aaaatgctgc tgggtgttta tgcctacttc atagagcata agcagcgcaa cacccttatc
      181 tggttgccga cggatggtga tgccgagaac tttatgaaaa cccacgttga gccgaccatc
      241 cgcgatattc cgtcgctgct ggcgctggct ccgtggtatg gcaaaaagca ccgggataac
      301 acgctcacca tgaagcgttt ttccaatggt cgtggcttct ggtgcctggg cggtaaagcg
      361 gcaaaaaact accgtgaaaa gtcggtggat gtggcgggtt atgatgaact tgctgccttt
      421 gatgaggata ttgaacagga aggctctccg acgttcctgg gcgataagcg tattgaaggc
      481 tcggtctggc caaagtccat ccgtggctcc acgcccaaag tgagaggcac ctgccagatt
      541 gagcgtgcag ccagtgaatc cccgcatttt atgcgttttc atgttgcctg cccgcactgc
      601 ggggaggagc agtaccttaa atttggcgat aaagagacgc cgtttggcct caaatggacg
      661 ccggatgacc cctccagcgt gttttatctc tgcgagcata acgcctgcgt catccgtcag
      721 caggagctgg acttcactga tgcccgttat atctgcgaaa agaccgggat ctggacccgt
      781 gatggcattc tctggttttc gtcatccggt gaagagattg aaccgcctga cagcgtgacc
      841 tttcacatct ggacggcgta cagcccgttc accacctggg tgcagattgt caaagactgg
      901 atgaagacga aaggggatac gggaaaacgt aaaaccttcg tgaacaccac gctcggtgag
      961 acgtgggaag cgaaaatcgg tgaacgtccg gatgctgaag tgatggcaga gcggaaagag
     1021 cattattcag cgcccgttcc tgaccgtgtg gcttacctga ccgccggtat cgactcccag
     1081 ctggatcgct acgaaatgcg cgtttgggga tgggggccgg gtgaggaaag ctggctgatt
     1141 gaccggcaga ttattatggg ccgccacgac gatgaacaga cgctgcagcg tgtggatgag
     1201 gccatcaata aaacctatac ccgccggaat ggtgcagaaa tgtcggtatc ccgtatctgc
     1261 tgggatactg gcgggattga ccctaccatt gtgtatgaac gctcgaaaaa acatgggctg
     1321 ttccgggtga tccccattaa aggggcatcc gtctacggta agccggtggc caacatgcca
     1381 cgtaagcgaa acaaaaacgg ggtttacctt accgaaatcg gtacggatac cgcgaaagag
     1441 cagatttata accgcttcac actgacgccg gaaggggatg aaccgcttcc cggtgccgtt
     1501 cacttcccga ataacccgga tatttttgat ctgaccgaag cgcagcagct gactgctgaa
     1561 gagcaggtcg aaaaatgggt ggatggcaga aaaaaaatac tgtgggacag caaaaagcga
     1621 cgcaatgagg cgctcgactg cttcgtttat gcgctggcgg cgctgcgcat cagtatttcc
     1681 cgctggcagc tggatctcag tgcactgctg gcgagcctgc aggaagagga tggtgcagca
     1741 accaacaaga aaacactggc agattacgcc cgtgccttat ccggagagga tgaatgacgc
     1801 gacaggaaga acttgccgct gcccgtgcgg cactgcatga cctgatgaca ggtaaacggg
     1861 tggcaacggt acagaaagac ggacggcgag tggagtttac ggccacttcc gtgtctgacc
     1921 tgaaaaaata cattgcggag ctggaagtgc agaccggcat gacacagcga cgcaggggac
     1981 ctgcaggatt ttatgtatga aaacgtccac cattcccacc cttctggggc cggacggcat
     2041 gacatcgctg cgtgaatatg ccggttatca cggcggtggc agcggatttg gtgggcagtt
     2101 gcgggcgtgg aacccaccgg gtgaaagtgt ggatgcagcc ctgctgccca attttacccg
     2161 tggcaatgcc cgtgcagacg atctggtacg caataacggc tatgccgcca acgccatcca
     2221 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     2281 ctatctgggc atcggggagg aagacgcccg tgccttttcc cgcgaggttg aagcggcatg
     2341 gaaagagttt gccgaagatg actgttgctg cattgacgtt gagcgaaaac gcacgtttac
     2401 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgagctgt tcgttcaggc
     2461 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     2521 gcgcatcagc aacccgaaca ataccggaga cagccggaac tgccgtgccg gtgtgcagat
     2581 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     2641 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgcct cgttcattca
     2701 cgtttttgaa cccgtggagg acgggcagac tcgcggtgca aatgtgtttt acagcgtgat
     2761 ggagcagatg aagatgctcg acacgctgca gaacacgcag ctgcagagcg ccattgtgaa
     2821 ggcgatgtat gccgccacca ttgagagtga gctggatacg cagtcagcga tggattttat
     2881 tctgggcgcg aacagtcagg agcagcggga caagctgacc ggctggattg gtgaaattgc
     2941 cgcgtattac gccgcagcac cggtccggct gggaggcgca aaagtgccgc acctgatgcc
     3001 gggtgactca ctgaacctgc agacggctca ggatacggat aacggctact ccgtgtttga
     3061 gcagtcactg ttgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg
     3121 gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta
     3181 ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg
     3241 gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagctt
     3301 tcaggaagcc cgcagtgcct gggggaactg cgactggata ggctccggtc gtatggccat
     3361 cgatggtctg aaagaagttc aggaagcggt gatgctgata gaagccggac tgagcaccta
     3421 cgagaaagag tgcgcgaaac gcggtgacga ctatcaggaa atttttgccc agcaggtccg
     3481 tgaaacgatg gagcgccgcg cggctggtct taaaccgccc gcctgggcgg ctgcagcatt
     3541 tgaatccggg ctgcgacaat caacagagga ggagaagagt gacagcagag ctgcgtaatc
     3601 tcccgcatat tgccagcatg gcctttaatg agccgctgat gcttgaaccc gcctatgcgc
     3661 gggttttctt ttgtgcgctt gcaggccagc ttgggatcag ccgcctgacg gatgcggtgt
     3721 ccggcgacag cctgactgcc caggaggcac ccgcgacgct ggcattatcc ggtgatgatg
     3781 acggaccacg acaggcccgc agttatcagg tcatgaacgg catcgccgtg ctgccggtgt
     3841 ccggcacgct ggtcagccgg acgcgggcgc tgcagccgta ctcggggatg accggttaca
     3901 acggcattat cgcccgtctg caacaggctg ccagcgaccc gatggtggac ggcattctgc
     3961 tcgatatgga cacaccgggc ggaatggtgg cgggagcatt tgactgcgct gacatcatcg
     4021 cccgtgtgcg tgacataaag ccggtatggg cgctggccaa tgacatgaac tgcagcgcag
     4081 gtcagctgct tgccagtgcc gcctcccggc gtctggtcac gcagaccgcc cggacaggct
     4141 ccatcggcgt catgatggct cacagtaatt acggcgctgc gctggagaaa cagggcgtgg
     4201 aaatcacgct gatttacagc ggcagccata aggtggatgg caacccctac agccatctac
     4261 cggatgatgt ccgggaaaca ctgcagtccc ggatggatgc aacccgccgg atgtttgcac
     4321 agaaggtgtc ggcatatacc ggcctgtctg tgcaggctgt gctggatacc gaggctgcag
     4381 tgtacagcgg tcaggaggcc attgatgccg gactggctga tgaacttgtt aacagtaccg
     4441 atgcgatcac cgtcatgcgt gatgcactgg atgcacgtaa atcccgtctc tcaggagggc
     4501 gaatgaccaa agagactcaa tcaacaactg tttcagccac tgcttcgcag gctgacgtta
     4561 ctggcgtggt gcaagtgacg gagggcgaga acgccagcgc ggcgcagccg gacgtaaacg
     4621 cgcagatcac cgcagcggtt gcggcagaaa acagtcgcat tatggggatc ctcaactgtg
     4681 aggaggctca cggacgtgaa gaacaggcac gcgtgctggc cgaaaccccc ggtatgaccg
     4741 tggaaacggc ccgccgcatt ctggcagctg caccacagag tgcacaggcg cgcagtgaca
     4801 ctgcgctgga tcgtctgatg cagggggcac cggcaccgct ggctgcaggt aacccggcat
     4861 ctgatgccgt taacgatttg ctgaacacac cagtgtaagg gatgtttatg acgagcaaag
     4921 aaacctttac ccattaccag ccgctgggca acagtgaccc ggcacatacg gcaaccgcgc
     4981 ccggcggatt gagtgcgaaa gcgcctgcaa tgaccccgct gatgctggat acctccaccc
     5041 gtaagctggt tgcgtgggat ggcaccaccg acggtgctgc cgttggcatt ctggcggttg
     5101 atgctgacca gaccagcacc acgctgacgt actacaagtc cggcacgttc cgttatgagg
     5161 atgtgctctg gccggaggct gccagcgacg agacgaaaaa acggaccgcg tttgccggaa
     5221 cggcaatcag catcgtttaa ccttaccctt catcactaaa ggccgcctgt gcggcttttt
     5281 ttacgggatt tttttatgtc gatgtacaca accgcccagc tgctggcggc aaatgagaag
     5341 aaatttaagt ttgatccgct gtttctgcgt ctctttttcc gtgagagcta tcccttcacc
     5401 acggagaaag tctatctctc acaaattccg ggactggtaa acatggcgct gtacgtttcg
     5461 ccgattgttt ccggtgaggt tatccgctcc cgtggcggct ccacctctga atttacaccg
     5521 ggatatgtca aacccaagca tgaggtgaat ccgcagatga ccctgcgtcg cctgccggat
     5581 gaagatccac agaatctggc ggacccggct taccgccgcc gtcgcatcat cctgcagaac
     5641 atgcgagacg aagagctggc cattgctcag gtcgaagaga tgcaggcagt ttctgccgtg
     5701 cttaagggca aatacaccat gaccggtgaa gccttcgatc cggttgaagt ggatatgggc
     5761 cgcagtgtgg cgaacaacat cacgcagtcc ggcggtacgg agtggagcaa gcgtgacaag
     5821 tccacgtatg acccgaccga cgatatcgaa gcctacgcgc tgaacgccag cggtgtggtg
     5881 aatatcatcg tgtttgatcc gaaaggctgg gcgctgttcc gttccttcaa agccgtcaag
     5941 gagaagctgg atacccgtcg cggctctaat tccgagctgg agacagcggt aaaagacctg
     6001 ggcgaagcgg tgtcctataa ggggatgtat ggcgatacgg cgatcgtcgt gtattccgga
     6061 cagtatgtgg aaaacgacgt caaaaagaac ttccttccgg acaacacgat ggtgctgggg
     6121 aacactcagg cacgcggtct gcgcacctat ggctgcattc aggatgcgga cgcacagcgc
     6181 gaaggtatta acgcctctgc ccgctacccg aaaaactggg tgaccaccgg cgatccggcg
     6241 cgtgagttca ccatgattca gtcagcaccg ctgatgctgc tggctgaccc tgatgcgttc
     6301 gtgtccgtac aactggcgta atcatggccc ttcggggcca ttttctctct gtggaggagt
     6361 ccatgacgaa agatgaactg attgcccgtc ttcaggtgct gggtgagcaa ctgaaccgtg
     6421 atgtcagcct gacggggacg aaagaagaac tggcactccg tgtggcagag ctggaagagg
     6481 agcttgatga cacggatgac gctgccggtc aggacacatc tgtcagcccg gaaaatgcgc
     6541 tgaccgggca tgaaaatgag gtggtatcag cgcagccgga taccgtgatt gatacggctg
     6601 ctctggtcac ggtcgtggca ctggtgacgc tgcatactga tgcacttcac gccacgcggg
     6661 atgagcctgt ggcatttgtg ctgccgggaa cggcgtttcg tgtctctgcc ggtgtggcag
     6721 ccgaaatgac agaacatggc ctggccagaa tgcaataacg ggaggcgctg tggctgattc
     6781 cgataacctg ttcgatgctg ccattgcccg cgccgatgaa acgatacgcg ggtacatggg
     6841 aacgtcagcc accatgacat ccggtgagct gtccggtgct gtgatacgtg gtgtttttga
     6901 tgaccctgaa aatatcagct atgccggaca gggggtgcgc gttgaaggct ccagcccgtc
     6961 cctgtttgtc cggactgatg atgtgcggca gctgcggcgt ggagacacac tgaccatcgg
     7021 cgaggaaaac ttctgggtgg accgggtttc gccggatgat ggcggaagct gtcatctctg
     7081 gcttgggcgg ggcgtaccgc ctgccgttaa ccgtcgccgc tgaaaggggg atgtatggcc
     7141 ataaaaggtc ttgagcaggc cgttgaaaac ctcagccgta tcagcagaac ggcggtgccc
     7201 ggtgccgccg caatggccat taaccgcgtt gcttcatccg cgatatcgca gtcggcgtca
     7261 caggttgccc gtgagacaaa ggtacgccgg aaactggtaa aggaaagggc caggctgaaa
     7321 agggccacgg tcaaaaatcc gcaggccaga atcaaggtta accgggggga tttgcccgta
     7381 ataaagctgg gtaacgcgcg gattgtcctg tcccgacgca ggcgtcgtaa aaaggggcag
     7441 cgttcagccc tgaaaggtgg cggcagcgtg cttgtggtgg gaaaccgtcg tattcccggc
     7501 gcgtttattc agcaactgaa aaatggccgg tggcatgtca tgcagcgtgt ggccgggaaa
     7561 aaccgttacc ccattgatgt ggtgaaaatc ccgatggcgg tgccgctgac cacggcgttt
     7621 aaacaaaata ttgagcggat acggcgtgaa cgtcttccga aagagctggg ctatgcgctg
     7681 cagcatcaac tgaggatggt aataaagcga tgaaacatac tgaactccgt gcagccgtac
     7741 tggatgcact ggagaagcat gacaccgggg cgacgttttt tgatggtcgc cccgctgttt
     7801 ttgatgaggc ggattttccg gcagttgccg tttatctcac cggcgctgaa tacacgggcg
     7861 aagagctgga cagcgatacc tggcaggcgg agctgcatat cgaagttttc ctgcctgctc
     7921 aggtgccgga ttcagagctg gatgcgtgga tggagtcccg gatttatccg gtgatgagcg
     7981 atatcccggc actgtcagat ttgatcacca gtatggtggc cagcggctat gactaccggc
     8041 gcgacgatga tgcgggcctg tggagttcag ccgatctgac ttatgtcatt acctatgaaa
     8101 tgtgaggacg ctatgcctgt accaaatcct acaatgccgg tgaaaggtgc cgggaccacc
     8161 ctgtgggttt ataaggggag cggtgaccct tatgcgaatc cgctttcaga cgttgactgg
     8221 tcgcgtctgg caaaagttaa agacctgacg cccggcgaac tgaccgctga gtcctatgac
     8281 gacagctatc tcgatgatga agatgcagac tggactgcga ccgggcaggg gcagaaatct
     8341 gccggagata ccagcttcac gctggcgtgg atgcccggag agcaggggca gcaggcgctg
     8401 ctggcgtggt ttaatgaagg cgatacccgt gcctataaaa tccgcttccc gaacggcacg
     8461 gtcgatgtgt tccgtggctg ggtcagcagt atcggtaagg cggtgacggc gaaggaagtg
     8521 atcacccgca cggtgaaagt caccaatgtg ggacgtccgt cgatggcaga agatcgcagc
     8581 acggtaacag cggcaaccgg catgaccgtg acgcctgcca gcacctcggt ggtgaaaggg
     8641 cagagcacca cgctgaccgt ggccttccag ccggagggcg taaccgacaa gagctttcgt
     8701 gcggtgtctg cggataaaac aaaagccacc gtgtcggtca gtggtatgac catcaccgtg
     8761 aaaggcgttg ctgcaggtaa ggtcaacatt ccggttgtat ccggtaatgg tgaacttgct
     8821 gcggttgcag aaatcactgt caccgacagt taatccggag agtcagcgat gttcctgaaa
     8881 accgaatcat ttgaacataa cggcgtgacc gtcacgcttt ctgaactgtc agccctgcag
     8941 cgtattgagc atctcgccct gatgaaacgg caggcagaac aggcggagtc agacagcaac
     9001 cggaagttta ctgtggaaga cgccatcaga accggtgctt ttgtggtggc tatgtccctg
     9061 tggcataacc atccgcagaa gacaaagcag ccttccatga atgaagccgt taaacagatt
     9121 gagcaggaag tgcttaccac ctggcccaca gaggcaattt ctcatgctga aaacgtggtg
     9181 taccggctgt ccggtatgta tgagtttgtg gtgaatgatg cccctgaaca ggcagaggac
     9241 gccgggcctg cagagcctgt ttctgcggga aagtgttcga cggtgagctg agttttgccc
     9301 tgaaactggc gcgtgagatg gggcgacccg actggcgcgc catgcttgcc gggatgtcat
     9361 ccacggagta tgccgactgg caccgctttt acagtaccca ttattttcat gatgttctgc
     9421 tggatatgca cttttccggg ctgacgtaca ccgtgctcag cctgtttttc agcgatccgg
     9481 atatgcatcc gctgga
//
LOCUS       DAZFVO020000179         9344 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFVO020000179 DAZFVO020000000
VERSION     DAZFVO020000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.180"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..169)
                     /locus_tag="ACHKNB_RS21430"
                     /old_locus_tag="pgaptmp_004286"
     CDS             complement(<1..169)
                     /locus_tag="ACHKNB_RS21430"
                     /old_locus_tag="pgaptmp_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197681.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKR"
     gene            247..1341
                     /gene="ddlA"
                     /locus_tag="ACHKNB_RS21435"
                     /old_locus_tag="pgaptmp_004287"
     CDS             247..1341
                     /gene="ddlA"
                     /locus_tag="ACHKNB_RS21435"
                     /old_locus_tag="pgaptmp_004287"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGU4197682.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1365..1577)
                     /gene="yaiZ"
                     /locus_tag="ACHKNB_RS21440"
                     /old_locus_tag="pgaptmp_004288"
     CDS             complement(1365..1577)
                     /gene="yaiZ"
                     /locus_tag="ACHKNB_RS21440"
                     /old_locus_tag="pgaptmp_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HGU4197683.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1837..2145
                     /gene="yaiY"
                     /locus_tag="ACHKNB_RS21445"
                     /old_locus_tag="pgaptmp_004289"
     CDS             1837..2145
                     /gene="yaiY"
                     /locus_tag="ACHKNB_RS21445"
                     /old_locus_tag="pgaptmp_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HGU4197684.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2204..3298)
                     /gene="yaiW"
                     /locus_tag="ACHKNB_RS21450"
                     /old_locus_tag="pgaptmp_004290"
     CDS             complement(2204..3298)
                     /gene="yaiW"
                     /locus_tag="ACHKNB_RS21450"
                     /old_locus_tag="pgaptmp_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HGU4197685.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3311..4531)
                     /gene="sbmA"
                     /locus_tag="ACHKNB_RS21455"
                     /old_locus_tag="pgaptmp_004291"
     CDS             complement(3311..4531)
                     /gene="sbmA"
                     /locus_tag="ACHKNB_RS21455"
                     /old_locus_tag="pgaptmp_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HGU4197686.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4883..6040
                     /gene="ampH"
                     /locus_tag="ACHKNB_RS21460"
                     /old_locus_tag="pgaptmp_004292"
     CDS             4883..6040
                     /gene="ampH"
                     /locus_tag="ACHKNB_RS21460"
                     /old_locus_tag="pgaptmp_004292"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HGU4197687.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6041..6664)
                     /gene="iprA"
                     /locus_tag="ACHKNB_RS21465"
                     /old_locus_tag="pgaptmp_004293"
     CDS             complement(6041..6664)
                     /gene="iprA"
                     /locus_tag="ACHKNB_RS21465"
                     /old_locus_tag="pgaptmp_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HGU4197688.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6752..>9344)
                     /locus_tag="ACHKNB_RS21470"
                     /old_locus_tag="pgaptmp_004294"
     CDS             complement(6752..>9344)
                     /locus_tag="ACHKNB_RS21470"
                     /old_locus_tag="pgaptmp_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4197689.1"
                     /translation="DYYHDRLALTVDNSTIDDNYEHYTYNGTYNNAADTHVVDVYNIG
                     TAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSSEVFNNTITVKDST
                     VTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAADNAMQTTATFDNST
                     LMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTLNNGSKWVGAAMSA
                     HLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANSLWPSSTVGVENSD
                     SEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADSTLIS
                     DSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKVTND
                     GILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTNDTTK
                     SNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRIDNA
                     TGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDY
                     ANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYD
                     QDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFAN
                     NVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYG
                     SISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVY
                     DDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSAN
                     VGVKYTW"
BASE COUNT         2378 a         2342 c         2247 g         2377 t
ORIGIN      
        1 ttctcttcgt taacttataa ttaagagaaa aaaactcacc gcatggccat ttaagccagg
       61 aataaattag cttcccgttc atcatttttg tgatcttgcg cacatctttg agataacgca
      121 ctttcttcac cagtacgttg cacgcttcgt cagcagaaga ttgcaacaat ctttcagaag
      181 gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt tgtgttagac gggataggtt
      241 tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg gtaaatcagc ggaacatgaa
      301 gtgtctctgc aatcggcaaa aaacattgtc gatgccattg ataaaagtcg cttcgacgtt
      361 gtgctgctgg gcattgataa acaagggcaa tggcacgtca gcgatgccag caattatctg
      421 ctaaatgcag acgatcctgc ccatattgcg ttacgccctt cggcgaccag ccttgcgcag
      481 gtgccaggta aacatgagca tcagcttatc gacgcgcaaa acggtcagcc gttgctgacg
      541 gtggatgtca ttttcccgat tgtccacggt acgctgggcg aagatggttc cttgcaggga
      601 atgctgcggg tcgccaattt accgtttgta ggttctgatg ttctggcttc agcagcctgt
      661 atggataaag atgtcaccaa acgtctgcta cgcgatgccg ggctgaacat tgcgccattt
      721 attactctga cgcgcgctaa tcgtcacaac atcagttttg ccgaagtgga gtctaaactg
      781 gggttaccac tgtttgtaaa accggctaat cagggctctt ctgttggtgt cagcaaagta
      841 accagtgaag aacagtacgc aattgccgtc gatctggcgt tcgagttcga tcataaagtg
      901 atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg cagttctggg caacgacaat
      961 ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg atttctatgc ctacgacacc
     1021 aagtacattg acgaagatgg cgcgaaagtg gtagttccgg cagccattgc accagagatc
     1081 aacgataaga tccgggcgat tgccgttcag gcttatcaga cgttgggatg cgcaggcatg
     1141 gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg tgatcaacga gatcaacaca
     1201 ctgcctggct tcaccaacat cagtatgtat ccgaagctgt ggcaagccag cggtctgggt
     1261 tacaccgatc tgatcacacg tttgattgag ctggcgctgg agcgtcacgc tgcggataac
     1321 gcattgaaaa ccacaatgta atattttaac gcccgataat attcttattg ggcgttttca
     1381 gtttcttcat cgcgacgacg aataatcaac cccgccagcc agaaactaat cacccacgtc
     1441 accagaccga cggcataggt ctgccaacct tttgcttcaa atcccagtag ccccaccacg
     1501 ccattaagaa tgaatataag gccaatggcg aacgcatagt agtgccagtc acggcggatt
     1561 tttacaggca gattcatggc agctccgtta acgaaaaaca atatccttgc atattctgta
     1621 acaaccgtct gtggtgtaga caacattttt gtaattgtta ataaatttaa cttaaggttg
     1681 ccattttgtg actcacaacg aagattcacg cttcgggaat attcccaaca tcaatttacc
     1741 atgattctga tattgacaat ctaaatgacc tgtcagactt gccataagcc gctggtatca
     1801 caccagaaga gaagcagatg acttccggag gtttttatgg ctgatttcac cctgtcaaaa
     1861 tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg
     1921 ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg
     1981 cccggcgtct atgagcgttt aatgcaggtc caggaaacag gtcgcccacg ggtggaaatt
     2041 ggtttaggtg tcggcaccat tttcgggctg atcccgtttt tagtaggctg cctcattttt
     2101 gcagtggtgg cgctatggct gcactggcga catcgccgcc agtaagattt acgttgccag
     2161 atacggccta aaacgcctta tctggcatga cattcctgtt tgatcactgt ttcatacaac
     2221 gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg cgggtgattt
     2281 tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg tagtgattta cccgttttcg
     2341 cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac
     2401 ctttctctaa ctgacggcga atttcgctgt cgctcatccc cagttttccc gccagtttgc
     2461 gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa tcaccgtcca
     2521 gtgccagctt cacgccgctg gccttactga ctgcgttctg aaacgcggca ttacggctgg
     2581 cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg tgcgctatag ctggcgggat
     2641 aattcagtaa atggtaagta ccaaaccaca acccgccgcg gcgactgaag acttcctgac
     2701 gaactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca aaagcaatgc
     2761 tgacctgcat cggcccaccg gtgcgcaccg gattaagcga accaaacagc gtctgcccca
     2821 ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct gtacgcaccg
     2881 aatctaaccg ttcgctataa cttttgccgt ttggcgattt gatttttagc gcggtatgaa
     2941 ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc caggcgatct
     3001 tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc gccaccgcca
     3061 gcaccgagca gacgttttcc agcgtcggtg ccagcccctg actttcaaag gtggtggcga
     3121 gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc cgcacgacgc
     3181 tcgctacatc tatcgctttt tcgccctttt tcaacggttg cggagcctga ctactacacc
     3241 ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag atgattgacg cgcgacatta
     3301 cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca tccagctcat
     3361 gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg gtccatgagt
     3421 taatcaggta ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca
     3481 ggccgagcgt aatcgtaccg gcaacaattg acggaaacag caagaacaaa ccgaaaacgt
     3541 tatcgacctg caaatagaga atgcgggcga tgttgaaata catatagtga aaatagaggc
     3601 gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc gtcgcgcgcg
     3661 tggcatcgtc ttcaccataa accagctctt tacggtaggc ggcctctact cgctggtttt
     3721 taaactccag ccccggcagt ttgatcccta ccactgccag caatccggtc cccatcagcg
     3781 accagacgat agcggcaatc accagaccat acggaatgtg cccgacaatt ggcagctccg
     3841 gcacatgtgc ggagagcgtt accagcaccg gcaggaaggc gatcaacgtc atgatggcgt
     3901 tgataaaact gacgcccata ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca
     3961 cacgctgtgc ggccccttcg atatgacgca gttgttgcca gttcgccatg taatattcgt
     4021 tcatcgccgt acgccagcgg aacacgtagt gactgacaaa gaagttgttc agtacactga
     4081 tcaccaccgc gatcagcgca atccccagaa agacgcccac ttcgcggtaa aattgttcga
     4141 tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac ggtgcatacc
     4201 aggcgttgac ggcgaccccg acttccacca aaaaccaggt gacgaagatg atcagtgcag
     4261 taccgagtat tgaccagtat tgccaacggt gcgggctgta gataaaccag aaaaatgcaa
     4321 aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac
     4381 gcgcggcgct aatcgggatc tgcccggaag cgccggtgat acgcgccacc cagtctcccc
     4441 caccggcttg ccagaagata acggcaatca atgcccaaac aaaggccgag agaaaaaacg
     4501 ctcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc ttatcgtttt
     4561 aacaactgct gtgtattaac cgcatgacca ctacaaagcg tcagccaaat gaaaccgccc
     4621 attgtgaccc atagcaaggc gcttagttcg ccagcctgac cataagaatt gtttcgacgg
     4681 atttcacaaa ccaaagattg tttacataag cggattaaca cgcgcaggat cacaaatgca
     4741 ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta acactattat
     4801 tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc agttaccttc
     4861 attcaatcta tggacaccac cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc
     4921 ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt ttgcctctga
     4981 tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg ggatggcgct
     5041 ggttgttatc gacggtaacc agcgcgtctt tcgtagttat ggcgaaacgc gacctggtaa
     5101 taacgttcgc ccgcagctgg attcggtcgt gcgtattgct tccctcacca aactaatgac
     5161 cagtgaaatg ctggtgaaat tgctcgacca ggggaccgta aagttaaacg atccgctaag
     5221 taaatatgct ccgccaggcg cacgcgtgcc gacctacaat gggacgccca ttaccctagt
     5281 gaatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg
     5341 tccggtgttt gtctggccaa cgcgcgaaca acgctggaaa tacctttcta cggcgaagct
     5401 aaaagccgcg ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc tgctggcaga
     5461 tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc agattacccg
     5521 tccattgggg atgaaagaca ccacctacac cccctcaccg gatcagtgcc gccgtttaat
     5581 ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg
     5641 cgtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttgt catctgattt
     5701 ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc gcgcgcagtt
     5761 tacgaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt taggctgggt
     5821 atatatggcc ccgaaagagg gtcgtccggg gattattcag aagacaggag gtggtggcgg
     5881 tttcattacc tatatggcga tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac
     5941 tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc tggtgaccga
     6001 gctaagcggg aataaaccgt tggttatccc cgcatcctga ttaatactct gaaggcaaac
     6061 ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta cgcaaagcag
     6121 cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata taattcatca
     6181 caccaatacg tgatcgcaat tcttcattcc agtcaatcat tgataataat gttgcgcgga
     6241 tttgttcgta ggaattatgc ccaatgagct gcacgtcgcg taattccaga attctatttt
     6301 gccaggctaa ccagtaaaaa gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg
     6361 tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat ttgtatggta
     6421 tatcgttttt cattaaacca tcagccagcc ccataatata aggagcctgg gtaataccga
     6481 taagtacgtt ttcttctcta cgcagagaga taacgccctc taaaataacg aaagtatctt
     6541 cgctattgac atcactggaa aatataactt gcttttcatt attaaactcg aagcgcgtac
     6601 cgtatctgga caaacattta tcgagcttac caaattcctg aagaggttta actacagata
     6661 acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt atttagatta
     6721 aattaccaga atttcttcgg agtgaagaat attaccaggt atatttaaca cccacgttcg
     6781 cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga
     6841 agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac
     6901 cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag
     6961 ccagtttgaa gtacggagtc agagcctggt cttcgctgta ggtgaaggta taacctgcat
     7021 ctacacccag ttcataacgc atgctgtcgt aagactgacc gtcaactttc atgtcgttgc
     7081 tcagctggta gtcatcacca gactggaaca gaccagaaat gctgccgtaa ggagtcacgt
     7141 aaccagcatc acccagtttg aagtcgtaac cggctttcaa accgaagccc caagcgtcgg
     7201 agttggtgct accgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga
     7261 agtgagagta actcaagcta ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt
     7321 agatgtaggc agtctggctg tcttgatcca cctgaccaga acggtcattc atgtcacctt
     7381 tagcgaagcc tgcagccgca ccgacgatcc acttagcgtt gttaccgtca attttggtat
     7441 caacaccgac catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt
     7501 tgaagttacc accgaagtag cttacccatg cgccgccgtt atcagccagg ccatgacgag
     7561 agttggtcag acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag
     7621 acgggatgct cagcgccatg ttagcgtagt cggtcagctc catctgttgc agaacaacgg
     7681 tgttaccgcg ctgttcagcc tgataggtgt atgcacccag gtcagctttg ttagcagcag
     7741 agaaggtcgc gttgctgttt ttgtcgttga cgtagatcag ctcgttgccg ttgtagtcag
     7801 cgatagaacc agcaccagta gcgttgtcga tacgaacttt gtagttaccg gttgcagctg
     7861 caacatggtc accgttgtta tcaacttcgt tctgaccagc ttcgatagaa gcagtgttgt
     7921 cgttatcgcc gttaccgtta atggtcaggt gaccatcaga gttcattgcg attaagccat
     7981 aaccgtagtt tgacttggta gtatcgttgg tacggtcgtt ggtcaggtct gcatcaagta
     8041 cgaagcggtt gctttcgatg ttgaacacac cagcgtgaat gttaccactg gtatcagtag
     8101 tgctggtcag gttcagagta tcaacctgca gagcattcgc cgcagtgtta ccgacatcca
     8161 gaataccgtc attagtcact ttgatgttgt tggcatacag agcagcatca cctacagccc
     8221 aaccagcaga aacttcatca gaaatggtta cggtactgtt gtcgacagtc agtgaatcag
     8281 ttgcaacatg accatcttcg ttgatgttca gcgcagaaag acctgtcaga gagatagagt
     8341 cagaaatcag cgttgaatca gcaacattaa cagttgaacc actgttgatg ctcagagtat
     8401 caatcagaga agttttggtg gtatcccact gtgaaccggt attcagggtc acattgaaca
     8461 gaccgctctg gtatacttcg ttaccgatga tgtgatcaaa ttcgctgtat tcagagtcgc
     8521 tgttttctac accaacagtt gatgaaggcc acaggctgtt agctgcgatg tcgatcagag
     8581 ttgcagttgc ttcggtgcct gtactggtat caataacaac cccgttgtca ttgtaaacag
     8641 gaccgtaagc atctggcaca ccatcatcat tggtatcaac agttaagtgc gcagacattg
     8701 ccgcaccaac ccacttgctg ccattgttca gggtcagatc caggcgatca gtaccatccc
     8761 aaccgttagt atctacatca ccgtcagcat cacgatagct atcacgacca tgcgggaaga
     8821 agttctcatc aaagttactt gagaagaata catcgcccat cagtgtagaa ttatcgaatg
     8881 ttgcagtggt ttgcattgcg ttatcagcag caggatgtgc aataacggct aatgcgatat
     8941 catctctatt ccagtcaccg ccattgtaat cgctggcatt gccggtatta ccaaaccaac
     9001 cactagtacc ttcatcagac catgaaccag aggtcacagt agagtcttta acagtgatgg
     9061 tgttgttaaa cacttcgctg ctgttaacac cagtgctaac cgtgttgtcg tcgatatctt
     9121 cccactcata accctgagtc aacgtaatac ccgcaacgtg agagttatta gtgatggaca
     9181 gatcaacttc ctgatccaga gtaatagccg taccaatgtt gtaaacgtct acaacatgag
     9241 tatcagcggc attattgtaa gtgccgttat atgtgtagtg ttcgtagttg tcgtcgatcg
     9301 ttgaattatc aacagtcagc gccagacggt catggtaata atca
//
LOCUS       DAZFVO020000180         9218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFVO020000180 DAZFVO020000000
VERSION     DAZFVO020000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.181"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            19..972
                     /locus_tag="ACHKNB_RS21475"
                     /old_locus_tag="pgaptmp_004295"
     CDS             19..972
                     /locus_tag="ACHKNB_RS21475"
                     /old_locus_tag="pgaptmp_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HGU4197690.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPTSDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1181..2146
                     /gene="lipA"
                     /locus_tag="ACHKNB_RS21480"
                     /old_locus_tag="pgaptmp_004296"
     CDS             1181..2146
                     /gene="lipA"
                     /locus_tag="ACHKNB_RS21480"
                     /old_locus_tag="pgaptmp_004296"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HGU4197691.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2247..2450)
                     /gene="tatE"
                     /locus_tag="ACHKNB_RS21485"
                     /old_locus_tag="pgaptmp_004297"
     CDS             complement(2247..2450)
                     /gene="tatE"
                     /locus_tag="ACHKNB_RS21485"
                     /old_locus_tag="pgaptmp_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HGU4197692.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2579..3367)
                     /locus_tag="ACHKNB_RS21490"
                     /old_locus_tag="pgaptmp_004298"
     CDS             complement(2579..3367)
                     /locus_tag="ACHKNB_RS21490"
                     /old_locus_tag="pgaptmp_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HGU4197693.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3460..3843
                     /gene="crcB"
                     /locus_tag="ACHKNB_RS21495"
                     /old_locus_tag="pgaptmp_004299"
     CDS             3460..3843
                     /gene="crcB"
                     /locus_tag="ACHKNB_RS21495"
                     /old_locus_tag="pgaptmp_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGU4197694.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3898..4107)
                     /gene="cspE"
                     /locus_tag="ACHKNB_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3898..4107)
                     /gene="cspE"
                     /locus_tag="ACHKNB_RS21500"
                     /old_locus_tag="pgaptmp_004300"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4197695.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4282..4842)
                     /gene="pagP"
                     /locus_tag="ACHKNB_RS21505"
                     /old_locus_tag="pgaptmp_004301"
     CDS             complement(4282..4842)
                     /gene="pagP"
                     /locus_tag="ACHKNB_RS21505"
                     /old_locus_tag="pgaptmp_004301"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HGU4197696.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            5431..6816
                     /gene="dcuC"
                     /locus_tag="ACHKNB_RS21510"
                     /old_locus_tag="pgaptmp_004302"
     CDS             5431..6816
                     /gene="dcuC"
                     /locus_tag="ACHKNB_RS21510"
                     /old_locus_tag="pgaptmp_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HGU4197697.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(6857..7537)
                     /gene="dpiA"
                     /locus_tag="ACHKNB_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /gene_synonym="citB"
     CDS             complement(6857..7537)
                     /gene="dpiA"
                     /locus_tag="ACHKNB_RS21515"
                     /old_locus_tag="pgaptmp_004303"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HGU4197698.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(7506..9164)
                     /gene="dpiB"
                     /locus_tag="ACHKNB_RS21520"
                     /old_locus_tag="pgaptmp_004304"
     CDS             complement(7506..9164)
                     /gene="dpiB"
                     /locus_tag="ACHKNB_RS21520"
                     /old_locus_tag="pgaptmp_004304"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HGU4197699.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT         2348 a         2322 c         2090 g         2458 t
ORIGIN      
        1 taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt aagcagaaag
       61 tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct taaccttctg
      121 actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa agtgcttaat
      181 ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt ccctgaccca
      241 ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca tctacatgag
      301 tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat
      361 aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt ccttcctgtc
      421 atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc gcccaccagc
      481 gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa catgttttgc
      541 accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt aatttgccgc
      601 gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa cgctgcgcat
      661 gtacttctgt taccggaaga gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg
      721 tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc tgcactggtt
      781 gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt tagccgctgc
      841 tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga tttctctatc
      901 cactacaaca aattcagcct gcgtgatccc atcctgcacg gagtaataga tgttatccgt
      961 aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac
     1021 aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat
     1081 gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga
     1141 aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat
     1201 ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt
     1261 ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
     1321 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt
     1381 ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt
     1441 tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg
     1501 cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg ccgatatggc
     1561 gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca
     1621 gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac
     1681 tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
     1741 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc
     1801 tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga
     1861 aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag aaattattga
     1921 ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc aatatttgca
     1981 gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat
     2041 gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc
     2101 ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
     2161 cttacatctg tctgataaaa aacccgcttt ttgaagcggg ttttttgtat cgaacaagat
     2221 attgagggag cgtcctgctc gccacgtcac tctttatgag agagcttttc agcctgaaga
     2281 tcaacgtctg cgcctttttt cgccgcagca tcgtcatcat tcatcgcctt cttgaaccct
     2341 ttaatggccg ctccaaggtc tccgcccagc gtacgtaact tcttagtccc aaacagcaga
     2401 acgaccagcg ccgcaactac cagcagtttg gtaatactaa tctcacccat agataccttc
     2461 ttgacatata accggcaaca gaacgcgcct taaaaaccag agaatattaa cggtcattcg
     2521 acgcgcgcac acaatagcaa tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc
     2581 ataataattg cggcggcgca aagcgacggt tgtttaagac gggcagttgc gcgcgcacct
     2641 gacgcacacg ttcgggcgtc acttccgcca taatgagtgc aggcatttct gacgctgccg
     2701 caatggtgac gccaaaggga tctataatcc ggctttgacc gatatttttg ttcccgcact
     2761 cccccgccgc caccatataa caggtggtat ccagcgcacg agcggcaagc aacgttgacc
     2821 aatgatgctc tttgagcggc ccgcgaaccc aggcggcagg aagtaccagg atttcagctc
     2881 cctgtaatgc ctgtgccagc gccagctctg gaaagcgtaa gtcataacag gtcatcagac
     2941 cgaccttcat cccctccacc tcgagtaacg gagcgatttc attaccagca tcaacacggc
     3001 gtgattcctg aatggcaaat gcatcataga gatgcaattt ggcataacgg gcgacgatgt
     3061 ttcctgcctg tagcgccacc agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa
     3121 tcgtcagaat tgtcgtcatc atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt
     3181 cgccttccag cagctgtgct gatttaaccg atagatctgc atcatgatca tcgcgcgcca
     3241 gcaatgcttc cggcaggacg aacagcgata cgtcgttttc cgccgcctgc gccatcaacg
     3301 aggcacaaat ctcagcgttc ttttcccaca cagatgtaac agcaaactgt cctgcggcaa
     3361 ccagcattgc cacctcctgt cagagtcgta aaattcgcca acgacgatta ttcggcgtta
     3421 cacttatcac tcatacaaat caaatagcag gattttgcag tgttacaact tcttttagca
     3481 gtttttattg gcggtggtac gggaagcgtg gcgagatggc tgttaagtat gcgatttaac
     3541 ccgctgcatc aggcgattcc gttggggacg ctggcagcaa acctgattgg ggcattcatc
     3601 ataggaatgg gattcgcctg gttcagcagg atgacgaaca ttgatccagt gtggaaagta
     3661 ttaatcacca ccggattttg tggcggtcta acaaccttct caacattttc ggcagaagtg
     3721 gtgtttttgt tacaagaggg ccgctttggc tgggcgttac tgaacgtttt cgtcaacctt
     3781 ctggggtctt ttgccatgac agcactggct ttctggctgt tttcggcctc aaccgcacac
     3841 taaagaccaa aaaaaaaccc gctgattaag cgggttttga attcttgctg acgtatctta
     3901 cagagcgatt acgtttgcag cagaagggcc tttggcaccg ttagtgattt cgaactctac
     3961 gcgctgacct tcagcaagag ttttaaaacc attagtctgg attgcagaga agtgtacgaa
     4021 cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat cctttggact cattaaacca
     4081 cttaacgtta cctttaatct tagacatcaa aattaccttt acatgaaaaa tagacacaaa
     4141 tgctgtgtcg gttaccagta caccaattgt ggtacgcttt gtccagtgca actttactaa
     4201 aaagtgataa aagtcgcgtt atttttgatg ttacttattt tagctattga tttttaagaa
     4261 gttactaaaa cttcatttgt ctcaaaactg aaagcgcatc caggcaaagt acacattgcc
     4321 attgttgtag gtacccggaa tgtaggtcat ctgaaaagtc actgggccat aacccacgga
     4381 ggccaatggc agtagaaccg ggagagggat gtaattccag ttatcgcgtg ccgttacgcc
     4441 agcggtgaat cccagaccta aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc
     4501 ccatccgtat ccggcaatcg gttcccattt gttccacgag tccttaaatg ccatggcata
     4561 caggccatgc cagtttcctt tttcatccca acgcgacagg ccaaaaccgc caccccacgg
     4621 tcgctcgtta tagcgatcgg ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat
     4681 ggcaggaata tataaatcat aatgttcagg ctgttgccag gtttgtgcaa tattttctct
     4741 aaatgttgtc atccactcat ctgcgttagc aaaaacttta ccaacgctga ttaactgaat
     4801 aaaaacaaag gaaaagatag cgacatattt actcacgttc atttgtgacc ataaaacatt
     4861 tatcaaaaat ctactactag catagcaaag ctacaattaa cataacctta atagacccaa
     4921 cataaagaat aatctgaata gctgctctat ttatttccgc ttgttcaagt taaatatttc
     4981 cgcgcatcga ttaaagatga tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa
     5041 ccaagtcttt tttgatgaca aaatgcaatc aaggaaaaat tattttattt tttaacgaga
     5101 tacaacaatc atcttaacga agtatataat atatatcgtc aacctatgct catattagat
     5161 tattttttga gcgcacagcc gcagctaaca ccattgcaat taacaaattt gcatcaatcc
     5221 accatcaatt tgcacacatt attatgtgat aattgccaac cgctaaatat gcgttttgtt
     5281 atctatgtat aaaaacagca acttcaatgt cttaatggca gtttttcttg attttaatca
     5341 gcattcatcg ccaatttatt gggcatattt tttccttaag ctttaggaat tttttattat
     5401 ttactttggg gcctggagac aggaaaaatt atgctgacat tcattgaact ccttattggg
     5461 gttgtggtta ttgtgggtgt agctcgctac atcattaaag ggtattccgc cactggtgtg
     5521 ttatttgtcg gtggcctgtt attgctgatt atcagtgcca ttatggggca caaagtgtta
     5581 ccgtccagcc aggcttcaac aggctacagc gccacggata tcgttgaata cgttaaaata
     5641 ttactaatga gccgcggcgg cgacctcggc atgatgatta tgatgctgtg tggatttgcc
     5701 gcttacatga cccatatcgg cgcgaatgat atggtggtca agctggcgtc aaaaccattg
     5761 cagtatatta actcccctta cctgctgatg attgccgcct attttgtcgc ctgtctgatg
     5821 tctctggccg tctcttccgc aaccggtctg ggtgttttgc tgatggcaac cctatttccg
     5881 gtgatggtaa acgttggtat cagtcgtggc gcagctgctg ccatttgtgc ctccccggcg
     5941 gcgattattc tcgcaccgac ttcaggggat gtggtgctgg cggcgcaagc ttccgaaatg
     6001 tcgctgattg acttcgcctt caaaacgacg ctgcctatct caattgctgc aattatcggc
     6061 atggcgatcg cccacttctt ctggcaacgt tatctggata aaaaagagca catctctcat
     6121 gaaatgttag atgtcagtga aatcaccacc actgctcctg cgttttatgc cattttgccg
     6181 ttcacgccga tcatcggtgt actgattttt gacggtaaat ggggtccgca attacacatc
     6241 atcactattc tggtgatttg tatgctgatt gcctccattc tggagttcct ccgcagcttt
     6301 aatacccaga aagttttctc tggtctggaa gtggcttatc gcgggatggc agatgcgttt
     6361 gctaacgtgg tgatgctgct ggttgccgct ggggtattcg ctcaggggct tagcaccatc
     6421 ggctttattc aaagtctgat ttctatcgct acctcgtttg gttcggcgag tatcatcctg
     6481 atgctggtat tggtgattct gaccatgctg gcggcagtca cgaccggttc aggcaatgcg
     6541 ccgttttatg cgtttgttga gatgatcccg aaactggcgc actcttccgg cattaacccg
     6601 gcgtatttga ctatcccgat gctgcaggcg tcaaacctgg gtcgtaccct atcacccgtt
     6661 tctggcgtag tcgttgcggt tgccgggatg gcgaagatct cgccgtttga agtcgtaaaa
     6721 cgcacctcgg taccggtgct tgttggtttg gtgattgtta tcgttgctac agagctgatg
     6781 gtgccaggaa cggcagcagc ggtcacaggc aagtaaatag taatgccgga ggcaagtttc
     6841 ctccggcttt atcatgtcac ccactgtggt atatgcgttg cggtctgcca actttgccgt
     6901 gaacaatttc agcaataatc agatggcggc tggcgcaata ttcaagataa cgcctggcag
     6961 tggtgcgact gatggtcagt gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct
     7021 ctttaaacag ttttcgcacc gcgtttagcg tcaagggatc aatgccggtc ggcagttcgt
     7081 ctttaggttc accgcgagca taggcattaa acatctcatc aatttgcttc tggctggcgc
     7141 tatcaatact ttccagcata tgtttacgct ggcggaaacg ggttagcgtt tgccccagcc
     7201 gttcataggc aatgggctta atgagataat caaatacacc acaacgtacg gcttcagaca
     7261 ccgtttccat atcgctggct gcagtggtaa acaccacgtc gccgggataa tgcgcctgca
     7321 ccagttcatg caataaatta atccctctgc cgtcaggaag atagttatcg agcaagatta
     7381 gccccggctt aaaacgctcg atcatcattc gggcctgcgc caggtttccc gccagtaata
     7441 tctgactgaa tccgggaatg tgacgaatat attccgcatg catctctgcc agcggcgttt
     7501 cgtcctcaac gatcaatagg gttaatggag ctgtcattag gtttcacttt cggaatatag
     7561 attgaaaata aggtaccgca gggatcatta tcttcgagag tgataacacc accgcagcgc
     7621 gttacgtagc tggcaatcaa gtacaaccca atgccatgtt caccgggctc gtcagcacgc
     7681 gtactgaccc cctgctcaaa tattttgtct cgtagagact ctggaacgcc gcagccctga
     7741 tcggcgactt caatcaccac atcatcgcct tcatcgctga ggaataattc aacgatcttg
     7801 tttccttcat cgctacgcag gctggcttcg aaggcgttat caagtaaatt gcccacaatg
     7861 gctgcaaact cggtgctatc cagtcctggc ggcagttgcg aaagctggct accggggaca
     7921 atgatcattt ttagccccag ttcccgggcg cgctgcactt taccaaaaag cagccccgcc
     7981 acctggcgat cggcaaacgc ctcgcgcagg ctgtcaataa gctgttgctg ggcctgagac
     8041 tccccctgca ccatcgccag cacgcgatca tactctttca tctgcaacag accattgagc
     8101 gtcgacatcc aattgagatg ctcgtgtcgc aatgtacgaa ggctctcgac gtattgtttt
     8161 atttgcgtca gttgcgcatt gagggtggaa atttcgtctt tactacgaaa gctgataatg
     8221 gcccccagca aatcatcacc tgaacgaata gcttcccgat tggcaataac gctcagaccg
     8281 ttaaagttag ccaccacatc ctgacgtttt tcatcaatct gttcggtaaa gaaatcggcg
     8341 ggcctgacca cttcagcaat gggtttaccc aaccattgcc gtccgggaga gctcagcccc
     8401 agcatctttc ttgcgttacg attgatggcg gtaatgtaac catgcggatc caccgcaatc
     8461 agcccttcat aaaccgaact aaacagcgcc tcttgctgac ggaccacgcg tgcgatttgc
     8521 tttggctcca tgcccatcat ttgccgacgg atatgcgctg ccaggaacca cgacagcaac
     8581 atcagaatcc ctaacagcac gacaaacacg cctgccatcg gtaataaaaa ctcagcccgc
     8641 cagctatcga ttttactcac caggtagcca atcgacacca cgccgatgac ttttccatcg
     8701 tcatcaaaga ttggcgtttt ggcgcgcatc gccatcccca ttgacccttt cccggtaatg
     8761 aagtagctct cccctttctc cagcgcgccc tgtttggtga actgcatagg ataaccaatt
     8821 ttctccggat taggatggta aaggcggatc gagtgccggt ccccaatcac cacataatca
     8881 aaatcggtat ctctttgtaa tttgttagcg atggtcgcca gccgtttgta gtcacgcgtt
     8941 ttcaccgcag agatgacact gtcattggag gcaataattt tcgcctgatt cattgccatg
     9001 tcgcgtacat gaagcgttaa atagtcctca aaactggccg taaaatattg cgccagggct
     9061 gcaatgacaa atattgagaa caccagaatc agcaaaaaga tacgcagcgg aaatgccagt
     9121 ctttggaaaa atgcaaactg tttattctcg ttaagctgca acattgtttt ccttgcgggt
     9181 tgtcccgagc ctctttgacg gatgtaatta aaatgttg
//
LOCUS       DAZFVO020000181         9053 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFVO020000181 DAZFVO020000000
VERSION     DAZFVO020000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 9053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.182"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            557..1504
                     /gene="stxA1a"
                     /locus_tag="ACHKNB_RS21525"
                     /old_locus_tag="pgaptmp_004305"
     CDS             557..1504
                     /gene="stxA1a"
                     /locus_tag="ACHKNB_RS21525"
                     /old_locus_tag="pgaptmp_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4197700.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRGRGITHNKILWDSSTLGAILMRRTISS"
     gene            1514..1783
                     /gene="stxB1a"
                     /locus_tag="ACHKNB_RS21530"
                     /old_locus_tag="pgaptmp_004306"
     CDS             1514..1783
                     /gene="stxB1a"
                     /locus_tag="ACHKNB_RS21530"
                     /old_locus_tag="pgaptmp_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4197701.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            2288..4228
                     /locus_tag="ACHKNB_RS21535"
                     /old_locus_tag="pgaptmp_004307"
     CDS             2288..4228
                     /locus_tag="ACHKNB_RS21535"
                     /old_locus_tag="pgaptmp_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4197702.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVD
                     KPLYKDLIGRTKAALKKSPKNVLFAVVWMQGEFDFGGMPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAI
                     LRHAGRVALNAGASSTVSEVRPSSPSGAEVTGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            4364..4543
                     /locus_tag="ACHKNB_RS21540"
                     /old_locus_tag="pgaptmp_004308"
     CDS             4364..4543
                     /locus_tag="ACHKNB_RS21540"
                     /old_locus_tag="pgaptmp_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4197703.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            4584..4829
                     /locus_tag="ACHKNB_RS21545"
                     /old_locus_tag="pgaptmp_004309"
     CDS             4584..4829
                     /locus_tag="ACHKNB_RS21545"
                     /old_locus_tag="pgaptmp_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4197704.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGGGSASDSGDPTPDSDMMM"
     gene            4907..5122
                     /locus_tag="ACHKNB_RS21550"
                     /old_locus_tag="pgaptmp_004310"
     CDS             4907..5122
                     /locus_tag="ACHKNB_RS21550"
                     /old_locus_tag="pgaptmp_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4197705.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            5127..5660
                     /locus_tag="ACHKNB_RS21555"
                     /old_locus_tag="pgaptmp_004311"
     CDS             5127..5660
                     /locus_tag="ACHKNB_RS21555"
                     /old_locus_tag="pgaptmp_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4197706.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5935..6507
                     /locus_tag="ACHKNB_RS21560"
                     /old_locus_tag="pgaptmp_004312"
     CDS             5935..6507
                     /locus_tag="ACHKNB_RS21560"
                     /old_locus_tag="pgaptmp_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HGU4197707.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPIVEGVGLDWKTQYRKL
                     KQRFNSCMVEMTIQLPGDTQRRPVICLALRKLAGWLQTISPNKVKLKIRDKVIQYQEE
                     CDDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHV
                     SKIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            6507..6656
                     /locus_tag="ACHKNB_RS21565"
                     /old_locus_tag="pgaptmp_004313"
     CDS             6507..6656
                     /locus_tag="ACHKNB_RS21565"
                     /old_locus_tag="pgaptmp_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197708.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            6664..7101
                     /locus_tag="ACHKNB_RS21570"
                     /old_locus_tag="pgaptmp_004314"
     CDS             6664..7101
                     /locus_tag="ACHKNB_RS21570"
                     /old_locus_tag="pgaptmp_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4197709.1"
                     /translation="MNRVLCVVIIVLAVGCGALSLGLNHYRDNSIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            7300..7797
                     /locus_tag="ACHKNB_RS21575"
                     /old_locus_tag="pgaptmp_004315"
     CDS             7300..7797
                     /locus_tag="ACHKNB_RS21575"
                     /old_locus_tag="pgaptmp_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4197710.1"
                     /translation="MKAITLFNTPIRVDESGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            7794..8051
                     /locus_tag="ACHKNB_RS21580"
                     /old_locus_tag="pgaptmp_004316"
     CDS             7794..8051
                     /locus_tag="ACHKNB_RS21580"
                     /old_locus_tag="pgaptmp_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197711.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            8277..8651
                     /locus_tag="ACHKNB_RS21585"
                     /old_locus_tag="pgaptmp_004317"
     CDS             8277..8651
                     /locus_tag="ACHKNB_RS21585"
                     /old_locus_tag="pgaptmp_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HGU4197712.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPEKIEGLICSEHFFTAAQGDHQA
                     KEDDLIYNPEPYVEATPDALHLLTFCVLVLKNGFTVTGESACASPENFDAEIGRKIAR
                     QNAVNKIWMLEGYLLKQKLSEQ"
BASE COUNT         2410 a         1941 c         2466 g         2236 t
ORIGIN      
        1 gagggagaac ctctgtattt tatcagcgaa aaaatagttt acgatcgtaa aaatctgcat
       61 atcatgataa gagtggttac attgccacgc tgcttaaccc gccgatgcgc gggttttttt
      121 gtacccagaa tcctgtgagc tatacggaaa gtacacagaa aggaaggtgc gaccacaatt
      181 aataacaaaa tcttaaaaat cgcacatggc actattagtt ttctaaatat tgtgtatttt
      241 ttgtattgca ggatgaccct gtaacgaagt ttgcgtaaca gcattttgct ctacgagttt
      301 gccagcctcc cccagtggct ggctttttta tgtccgtaac atcctgtgta tcaataaatg
      361 ttgttgtcta cgtacgtcaa gtagtcgcat gagatctgac cagatatgtt aaggttgcag
      421 ctctctttga atataattat cattttcatt acgttattgt tacgtttatc cggtgcgccg
      481 taaaacgccg tccttcaggg ggtggaggat gtcaagaata tagttatcgt atggtgctca
      541 aggagtattg tgtaatatga aaataattat ttttagagtg ctaacttttt tctttgttat
      601 cttttcagtt aatgtggttg cgaaggaatt taccttagac ttctcgactg caaagacgta
      661 tgtagattcg ctgaatgtca ttcgctctgc aataggtact ccattacaga ctatttcatc
      721 aggaggtacg tctttactga tgattgatag tggcacaggg gataatttgt ttgcagttga
      781 tgtcagaggg atagatccag aggaagggcg gtttaataat ctacggctta ttgttgaacg
      841 aaataattta tatgtgacag gatttgttaa caggacaaat aatgtttttt atcgctttgc
      901 tgatttttca catgttacct ttccaggtac aacagcggtt acattgtctg gtgacagtag
      961 ctataccacg ttacagcgtg ttgcagggat cagtcgtacg gggatgcaga taaatcgcca
     1021 ttcgttgact acttcttatc tggatttaat gtcgcatagt ggaacctcac tgacgcagtc
     1081 tgtggcaaga gcgatgttac ggtttgttac tgttacagct gaagctttac gttttcggca
     1141 aatacagagg ggatttcgta caacactgga tgatctcagt gggcgttctt atgtaatgac
     1201 tgctgaagat gttgatctta cattgaactg gggaaggttg agtagtgtcc tgcctgacta
     1261 tcatggacaa gactctgttc gtgtaggaag aatttctttt ggaagcatta atgcaattct
     1321 gggaagcgtg gcattaatac tgaattgtca tcatcatgca tcgcgagttg ccagaatggc
     1381 atctgatgag tttccttcta tgtgtccggc agatggaaga ggccgtggga ttacgcacaa
     1441 taaaatattg tgggattcat ccactctggg ggcaattctg atgcgcagaa ctattagcag
     1501 ttgagggggt aaaatgaaaa aaacattatt aatagctgca tcgctttcat ttttttcagc
     1561 aagtgcgctg gcgacgcctg attgtgtaac tggaaaggtg gagtatacaa aatataatga
     1621 tgacgatacc tttacagtta aagtgggtga taaagaatta tttaccaaca gatggaatct
     1681 tcagtctctt cttctcagtg cgcaaattac ggggatgact gtaaccatta aaactaatgc
     1741 ctgtcataat ggagggggat tcagcgaagt tatttttcgt tgactcagaa tagctcagtg
     1801 aaaatagcag gcggagattc ataaatgtta aatacatctc aattcagtca gttgttgccg
     1861 gtctgataat agatgtgtta gaaaatttct gcatggtgaa tccccctgtg cggaggggcg
     1921 actggtgaac ggtatgatct ctttgatgat cgtaagcgag aatacgcggg tttggtggca
     1981 ccaggccgaa ctcaccggga ggcacccggc accatgcagt attcagagat taggcatata
     2041 tccaggctcc tcatcgcagg agccttttta catgcaaaaa aaaaccgctc ctgggaagag
     2101 cggctggcaa gaagaaacaa tatgaacaat taattaacga tgggaataat accttagagt
     2161 aatcaccttg cgcaactgta agggtatatt tcatctttgc gggctgtttt tctgtgtggc
     2221 ttctgtgttt ccggagggca gcttgtacct tttctgactc agaacattat cccggccggg
     2281 aggattcatg gcatttaaac actacgatgt ggtcagggcg gcatcgccgt cagaccttgc
     2341 tgaacgaata actcaaaaac tgaaggaagg gtggcagcct tatggtagtg cgctgatttc
     2401 gacagctggt tatggtgcag agtttatcca gccagttgtg agtgaggggg agcttccctc
     2461 acttgcagag tcaggcaatc acccgcatgt ttcagcgaag cctgaagcag cgccggaata
     2521 ttactatgtg attgcgcttg ccggtcagtc caatagcatg tcatacggtg aaggtcttcc
     2581 gttgccggag acatatgacc gtccggaccc gcgtattaag cagctggcgc gccgcagtac
     2641 ggtgacaccg ggcggtgccg catgcaaata taacgacatc attccggcgg accattgtct
     2701 gcatgatgtg caggacatga gccgccttaa ccatccgaaa gcggacctgt caaaggggca
     2761 gtacggaacc gtggggcagg gactgcatat cgccaaaaaa ctgctgccgt ttataccggc
     2821 gaatgcgggc attctgctgg ttccgtgctg tcgtggtggt tcagcgttca ccaccggagc
     2881 tgatggcaca tacagtgacg cgagtggtgc ctcggagaat tcaacccgct ggggtgtgga
     2941 caagccgctg tataaggacc ttatcggtcg aacaaaagca gcactgaaga agagcccgaa
     3001 aaatgtgctg tttgccgtgg tgtggatgca gggggaattt gattttggcg gtatgccggt
     3061 aaatcacgca gcacagtttg gcgcgctggt tgataaattc cgtgcagacc tggcggatat
     3121 ggcaggccag tgcgtcggtg gctctgctgg tggtgttccc tggatatgcg gggacacgac
     3181 gtatttctgg aagcagaaga acgaatccac gtaccagacg gtgtacggca gctataaaaa
     3241 caaaacggaa aagaatatcc atttcgtacc gttcatgacg gatgagaacg gggtgaatgt
     3301 gccgacgaac aaaccggaag aagacccgga cattccgggt atcgggtatt acggttcgaa
     3361 atggcgtgac agctcagcca cctggacgtc acaggacagg gcgagccatt tcagcacctg
     3421 ggcacgccgg gggattattt ccgaccgtct ggcaacggcg attctgcgcc atgcgggaag
     3481 agtggcgcta aacgcggggg catcatcgac agtatcagag gtgcgtccgt catcgccttc
     3541 cggtgcagaa gtcacaggcg tcacaacact gctctcttac cttgccagcg agtcagaggg
     3601 aagcctgaaa gtacagggat ggtcagccag tggcggcagg gcagaagtgg tcagcgatgc
     3661 ggagggaacc ggaggtaagg ctgtgaaggt gaccaaggaa gccggtaaaa gcagctgggt
     3721 gctggagtac gctgcaggca acggtgcggc tctgttacag aaaggggggc agattcgctg
     3781 ccgctttaag gcttcgggag cgctggctgc gaaccagtat gttatggcgt tttactggcc
     3841 ggtatcgtca ctgccacagg gcgttgtcct gaccggcgat ggggggaata acctgctggc
     3901 agcgttctac atccagacgg atgcaaaaga cctgaatgtg atgtaccaca atgcgaaagt
     3961 ggcgacaaac aacctgaaac tgggaagctt tggcgcattt gataacgaat ggcatacgct
     4021 ggctttccgc tttgccggga ataacagcct tcaggtgacg ccggttattg atggtcagga
     4081 tggcacaccg ttcacgctga cgcagtcacc ggtcagtgca ttttcggcgg ataagttgca
     4141 tgtgacagac attaccaaaa gtgcgactta cccggtactg atagacagca ttgcggtgga
     4201 agtgaataac gcggacgccg ctgcatgata aaaaaagccg ccagttaccg gaatggactg
     4261 gcggtggtaa tacctatgga gaaaaaataa aggaacgata ctttcgtact ctgttttttt
     4321 aaatgaaaac agttcttatt gtcaacaata acggaaagaa attatgacat ttctgaacca
     4381 gttaatgctg tacttctgta cggtggtctg tgtgctgtat ctcctttcgg gtggataccg
     4441 ggccatgcgt gacttctggc gcagacagat tgacaaaagg gccgctgaga aaatcagcgc
     4501 cagtcagtca gccggaagca aacccgaaga gccgctcgtt tagcggcaac tttcttaatc
     4561 acacctttcg acgagaaaat cccatgtcag aaattacatc cctggtcact gctgaagcag
     4621 tgaaggacgt cctgcgctct gaagaagtcc tgagcgcact gaaacagaaa cttcgccata
     4681 acctggaagc gcgtcttgat gcagaagtgg atgccattct ggatgaactg ctgggcgcac
     4741 cggcagctcc ggagccggaa ggcatcgcgg gaggggggag tgcttcagat agcggtgacc
     4801 ccacacctga cagcgacatg atgatgtaag catgcgtcag ggaccatcgg tgtgtgccgg
     4861 tggtcttttt tattgttgtg agcttccgga ttgcgggagg cggggtatgt accagatgga
     4921 aaaaatcaca acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct
     4981 gcagttgctg gacagggttt ccccgtctca gtgggcggca ataggcgtgc tggggagtct
     5041 gctgtttggt ctgctgacgt acctgacgaa cctgtatttc aaaatcagag aggaccggcg
     5101 taaggcggcg cggggagagt aagctgatga gccggaaatt ccgctatggt ttatcagctg
     5161 tcgttctggc gctgattgcc gcaggggctt ctgcgcctga aatcctggat cagtttctgg
     5221 atgaaaaaga aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct
     5281 gccgtggagc cacccgggtg gatggtaagc ctgtgattcc tggcatgaag ctgtcgaagg
     5341 aaaaatgcga ccgggttaac gctatcgaac gggataaggc gctggcatgg gtggagaaaa
     5401 acatcaaagt gccactgact gaaccccaga aagcgggtat tgcgtcattc tgtccttaca
     5461 acattggccc gggtaagtgt ttcccgtcga cgttttataa acgaattaat gcaggagatc
     5521 gaaaaggtgc ctgcgaagcg attcgctggt ggattaagga cggtggcagg gactgccgta
     5581 ttcgctcaaa taactgttat ggtcaggtat cccgtcgtga ccaggagagc gcgctggcgt
     5641 gctggggaat cgacagataa gcagaatatt ttgctgataa atgacgcttg ctcatgcggg
     5701 cggataacac gaaatcctgc gaactggcaa aatgcaagtg aataaagtca ggaagattgt
     5761 tttacgcaga ggcaccgtaa tggtgtcttt gtcatttctg cgcttcgcac aagcgtaaat
     5821 aaaccaaaga acctttcagg atgagccctg gtggataacc ggcagtggtc tggttaaccc
     5881 tctttgggct ggttattcct gtgcgcaggg ttcatcacta aaaggaaata accgatgaat
     5941 atgatgaccg tgccgtttca cggcgattct ctttatgtgg ttaaccataa tggcgaaccg
     6001 tatgttccaa tgaaaccgat cgttgaaggg gtggggctgg actggaaaac acagtatcgc
     6061 aagttaaaac aacggtttaa tagctgcatg gtcgaaatga ccattcagct accaggtgat
     6121 acccaacgtc gccctgttat ctgcctggct ttacgtaaac ttgctggctg gcttcagact
     6181 atcagcccaa ataaggttaa gctcaaaatc cgagacaagg tgatccagta tcaggaagag
     6241 tgcgacgatg ttctctatga atactggacg aagggttttg tcgttaatcc ccgtcgaatg
     6301 agtgtgatgg aagaactcaa tcaggcttgc gctgacatga aacgggataa aaacattgcc
     6361 agtgtgtttg ctaccgggct gaatgagtgg aaacaggtta aagccgcgca tgtatcaaaa
     6421 atccgcacat tgataaacga agcgaatctg ctgattgatt ttgtcctagc tgatacaggc
     6481 aaagggaaaa taacaaaggc ggattgatgg agtggtggct aatgatatcg gataaactca
     6541 taacgctggc gaagatcctc tgtgtaatcg tcggcatttc atttttagtc atgctggttg
     6601 ccattttctt ttccaccgcc tggagagtcc tgacgttatc gggactggtg gggtgaaaga
     6661 gagatgaacc gtgttctgtg tgtggtgatt attgtcctgg cggttggctg tggtgcgctt
     6721 agtctggggc tgaatcatta ccgtgataac tccatcgcct acaaagagca gcgcgataaa
     6781 gccacatcca tcatcgcaga tatgcagaag cggcaacgtg atgtagcaga acttgacgcc
     6841 agatacacaa aggagcttgc tgatgctaat gcgactatcg aaagtctccg tgctgatgtt
     6901 tctgctggtc gtaagcgcct gcaagtcgcc gccacctgtg caaagtcaac gaccggagcc
     6961 agcagcatgg gcgatggaga aagcccagga cttacagcag atgctgaact caattattac
     7021 cgtctcagaa gtggaatcga caagataacc gcgcaggtta actacctgca ggaatacatc
     7081 aggacgcaat gcctgaaata atttttttgc aaatcacaaa gtcaatttaa tgagcctcgc
     7141 gatgcggggc ttttttatgt ccgcagtaaa cgcgcatctc acgcgcatat taacgagagc
     7201 ctttcagtaa gcgggcctga gaattgccgt tataggtggc gacctctctc gggcggcttt
     7261 tctgtgagac aggctcactt tctaaaaggt aaagacgcca tgaaagcaat cacgcttttt
     7321 aatacaccga tccgtgttga tgaatcagga atgatctgcc tcactgatat gtggaaagcc
     7381 agtggtaaaa gtgaatctga atctccgtac cactacctgc gaaacaagca gaccaaagag
     7441 ttcttagccg agctggagaa aaaccacgaa tctgtggttt ttactgagcg cggtgtacac
     7501 ggtggaacat atggcgggaa gtttgttgct tatgattatg cagcatggct aaaccccgga
     7561 tttaaatatg cagcctataa agtcctggat gactacttca ccggagaact tcagcatcgc
     7621 aacagcttaa gtgcgcagct caatatgaag tgtcatgagt ttgatcagaa aaaagatatg
     7681 gcgagcttct gtgggcaagg tctggcggca tggcgctata cgaaaccagt gttggtcgct
     7741 gagattaact ccctggctaa ccagctgcag ataacgatcc ccgggcttcc gggatgagtg
     7801 atcgtgtcat tgaatgcgcc tccagagcgg ggcgcgactt ctcagagttc atgaaaggcg
     7861 agaagggcat gatggaagca ttggcctcgg tggatgagtt tggcgagcag ctgcgcctca
     7921 acggctgtgt caatcatcac tttgttagct acatgatgcg gaactcgatc atgcaggcat
     7981 tcatggacat ggcaaaagcc gagaggaaag aagagcgccg gcgtaagcga gcggaagcaa
     8041 aagcgaagta gccattacaa agcctaccta cgggtgggct tgataatgaa accggaattt
     8101 attctgggca accagttacg gcagtacagt gaaacaaccc aagccagtaa gtggggaaat
     8161 aacactggca gccactgaaa gatgaacctc cagccttatg gcaaaaaaga ttctttgtgg
     8221 tggcagactg atggaaagac atcctaatca agcaaccact ccacagggtc ataattatga
     8281 acgaccagca aatcgaaaaa gaaatcgttg agaaaggcaa aacggcaccg cgtgtaacac
     8341 ctgagaaaat tgaaggcctc atttgcagtg agcatttttt cactgctgcg cagggtgacc
     8401 accaggctaa agaagatgac cttatttata atccggagcc ttatgtcgaa gccacccctg
     8461 atgctctgca ccttcttact ttctgcgtat tggtactgaa gaatggcttc accgttaccg
     8521 gagagagtgc ctgtgcaagt ccggaaaatt ttgatgcaga aattggtcgg aagattgccc
     8581 ggcagaatgc tgtaaacaaa atctggatgc tcgaaggtta cttgctgaag cagaagctaa
     8641 gcgaacagta gttattacaa aagccattcc ctacagagtg gctttgataa tggcttatac
     8701 cctacacggg ataacttaat tgatatcctt tttaacggat aaaggcattt cagcctgaca
     8761 tagccatgcg ccgtatcgtt gccgtattcc tgcattaaca gagaccgcag cccgacagcg
     8821 agactcctct gcgtgagtgt gcggggataa tcaaaaacga tgcacaccgg gttttctcat
     8881 ttttcacgag atgggagcga tttcccgcga agccgcctgt ccggtgcggt ggtggaagaa
     8941 accggataaa acaaccgcat tgtgcaaata tcgatcaaat atggtgctgc tgtgtgaaat
     9001 ctgaaaaatc acagcggtca ttatgcatca gtttttaaca caggacgtta gaa
//
LOCUS       DAZFVO020000182         8824 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFVO020000182 DAZFVO020000000
VERSION     DAZFVO020000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.183"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1200..1388)
                     /locus_tag="ACHKNB_RS21590"
                     /old_locus_tag="pgaptmp_004318"
     CDS             complement(1200..1388)
                     /locus_tag="ACHKNB_RS21590"
                     /old_locus_tag="pgaptmp_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197713.1"
                     /translation="MVNLPPELGSPDVWAGGGIGLIAGLLLRYFIVRVFIVFRLFGRE
                     WSFDLKDRTRTDNHADPR"
     gene            1686..2237
                     /locus_tag="ACHKNB_RS21595"
                     /old_locus_tag="pgaptmp_004319"
     CDS             1686..2237
                     /locus_tag="ACHKNB_RS21595"
                     /old_locus_tag="pgaptmp_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197714.1"
                     /translation="MNDREFQKFLEESKRRNRHNGYSYTNNPTSYEVPAFSKTERKNI
                     EAVIRSITPRDRFMSVRKTTKNNLKPFLMSFDSYEQLPAKIEDLIIGTCRSFGRDNYH
                     RKVFYLLRNIDKISSSTITNYLQRQATRLSYELPSDKYCANLTTICMKVIETINHHVE
                     VGNISLTTSEPDFEFDPYILEGF"
     gene            2239..2517
                     /locus_tag="ACHKNB_RS21600"
                     /old_locus_tag="pgaptmp_004320"
     CDS             2239..2517
                     /locus_tag="ACHKNB_RS21600"
                     /old_locus_tag="pgaptmp_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197715.1"
                     /translation="MNKHTLTRISEEAFHELTRIKQTTNLPHQTIMQLAIAKEVTESD
                     LLKYPVSKGRRHIRITKNALATLKELNNFKIDIARLLSIKIHKLSMGV"
     gene            2517..2732
                     /locus_tag="ACHKNB_RS21605"
                     /old_locus_tag="pgaptmp_004321"
     CDS             2517..2732
                     /locus_tag="ACHKNB_RS21605"
                     /old_locus_tag="pgaptmp_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197716.1"
                     /translation="MLCITFEYHTDKMIRYISDLLIKGNGFGDIHNSKDIFIKAIGPN
                     ETLKTAVKPEWFERHKIELGYWGEEVL"
     gene            2729..3016
                     /locus_tag="ACHKNB_RS21610"
                     /old_locus_tag="pgaptmp_004322"
     CDS             2729..3016
                     /locus_tag="ACHKNB_RS21610"
                     /old_locus_tag="pgaptmp_004322"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4197717.1"
                     /translation="MKPWHEDVRRYFAEHLIYDESSDSLCWSDGESVTINTDDYGNKT
                     FNIGRYTFYVKYVVWFLYHGYQSNKQIIHRNGNRADTRPKNLMQVRDFKRN"
     gene            3173..3481
                     /locus_tag="ACHKNB_RS21615"
                     /old_locus_tag="pgaptmp_004323"
     CDS             3173..3481
                     /locus_tag="ACHKNB_RS21615"
                     /old_locus_tag="pgaptmp_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197718.1"
                     /translation="MNNYPMTVLIDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHT
                     INEIKLGDFVHKLEVTDPDYKRFITEECNRLDLSPEQLLKLNIFLTSQSELNPYSEVM
                     "
     gene            3976..4683
                     /locus_tag="ACHKNB_RS21620"
                     /old_locus_tag="pgaptmp_004324"
     CDS             3976..4683
                     /locus_tag="ACHKNB_RS21620"
                     /old_locus_tag="pgaptmp_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197719.1"
                     /translation="MENLYINGKKIDSFGDWSPQTEHPAIAIQRKEHDERIALEQELK
                     MTPKEITFVSPEPQEMPDVCKSEALLELEKKYFPALKAQRLRLDDAYSKVTLMQSAIE
                     PSEFEIQDELSQKPFVYYQYADNDGFGTFPENIPDVINNLPDGYRIVKMVKASRGAGQ
                     FVYMSNKSDEELAELARQNILASRNKQLENVKLYLSRELQAMKELVSEYESQKKIAMQ
                     ADIEQLVKISTKYAKVL"
     gene            4705..4914
                     /locus_tag="ACHKNB_RS21625"
                     /old_locus_tag="pgaptmp_004325"
     CDS             4705..4914
                     /locus_tag="ACHKNB_RS21625"
                     /old_locus_tag="pgaptmp_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197720.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKSKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            5028..7196
                     /locus_tag="ACHKNB_RS21630"
                     /old_locus_tag="pgaptmp_004326"
     CDS             5028..7196
                     /locus_tag="ACHKNB_RS21630"
                     /old_locus_tag="pgaptmp_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4197721.1"
                     /translation="MAQFKVDDFLIELGFNSQRVLKGLEKAEKQTMQVASRIEKRLNK
                     AFKVNPTPLNDSLKVMERNVDKTVSKIEQRLKNTKAFKIKTEIEDTLKPLRQQRQPRI
                     SGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGSMDSSKFNEKLAQLNASV
                     REAIAKARGHTSTSINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGLKRDS
                     AQRAAKFVLGDKASEAETFIRGLADKTGLNISEGLSSYAKFAAGAQGSMSQEQTQELF
                     GNATAMSRLMGLSNDELNGILKAFEQMASKGKIQAEELRGQLGDRMAGAFKLFAEALG
                     MTATELDKAMKDGKILSSDTLPKVAKQMGLMIDKAGGWAEVAKSTQTALGKLANNWDD
                     TMVKIFSGSQDELNGFLSRLSSLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRIFNNH
                     LEGWILQTKKLYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVMNLTR
                     AITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASMIPQI
                     RGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINEGWGR
                     IFSSMGELFNNATMNIARFNHPELITMKQDASLTSADIQGLRDEISALSKRIQEPVKV
                     SLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
     gene            7353..8141
                     /locus_tag="ACHKNB_RS21635"
                     /old_locus_tag="pgaptmp_004327"
     CDS             7353..8141
                     /locus_tag="ACHKNB_RS21635"
                     /old_locus_tag="pgaptmp_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HGU4197722.1"
                     /translation="MIYSNQIKYSGKDGIYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATG
                     KTATTNDGGVTSKKNAGNVTTERNNAPMLSCKELMNISDVSGYAPAIRNDILDAQIKC
                     NKAWKLDYSDLTEAEKTNVTNANSNAGRVASKLKKYSVNPNKRY"
     gene            complement(8172..>8824)
                     /locus_tag="ACHKNB_RS21640"
                     /old_locus_tag="pgaptmp_004328"
     CDS             complement(8172..>8824)
                     /locus_tag="ACHKNB_RS21640"
                     /old_locus_tag="pgaptmp_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HGU4197723.1"
                     /translation="DNPLTINIFKGLFRCQCGASVHPTGTKNKYQGVYRCNNHLDGRC
                     DVPPLKRKPFDKWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDITELKTQVKGLNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTDPLPLNDGTSIFEIAEKNLLNIDTFQL"
BASE COUNT         2848 a         1629 c         1851 g         2496 t
ORIGIN      
        1 agaaagtcaa tagatatttt aaaattctgc aaagggggtc acatagctgg tgtgacgggt
       61 acttagcagc tacagattga ttgcagatat tttcgaagat tatcggggaa gggaaatgta
      121 gaggggctag tcatttttta taaaaaattt agaggctgga tattgcctgt atagatttta
      181 accagtatag aagcctgtat aacatgaggg gttcctatgg tcactatatc cctctttccc
      241 actaaggaag ccttagcagc acctaggcaa ctattgacta aggtcaactt cataaactca
      301 gagctaagtt accttgaaag attctaccaa gaactacaca ggttttattt agtctgctat
      361 gaagggaata tttaacggga ggaagagaac catgaaaccg aaggtaggtt ctatcttaac
      421 agctactgta agagtaagac tgaaaaactc ttaacagata actacctaga aagaagggaa
      481 cgataagacc agaggtagtg acctgttaaa caggctgttt caaagtctct taagaaatct
      541 gttaaataag gtaatgtgag caatcttacc attttgtcaa tgcttttgtc tttggcctaa
      601 atcaataaaa tgtatcgttt gatatggttt taagcaaaat agctaaaaaa tctttttaga
      661 ggggcgcttc gcttggggtc actatgttct ttctctccat gtctaagagg gaagttacag
      721 ggcatttcaa agccctcaat agacaaaccg aggaacacgg aaaaccgagg gaagaaaact
      781 attgtgatta ccttgaacaa tgtctgtaaa gactggttag gaggtcgatt aagaagggag
      841 cttccctctc tggggttatc gctctctctc gcttagtgga ttgattaaga aggcttctga
      901 agtttctctg tgaaggtact ggacagcctt atatattggg tgattacaac tcattgaaaa
      961 ataagtgttt ttagaagtta ttgttaaggt tacagagtta actgtgaaga gacttgtcag
     1021 ttctctatat atgggtgatt acaacttatt gaaaaataag aacttttaga aattactgtt
     1081 agaggggtga atacttgata gatacagctt tccggttctg tcaagctatt ttttgataga
     1141 tagcacaaaa tgctaaagat accccctata catctcgtac aggggatttt gaagggagtt
     1201 taacgggggt ctgcgtggtt atctgttctg gtcctgtcct tgaggtcaaa cgaccattca
     1261 cgaccaaaca atctgaacac aataaacacc cgaacgatga aatagcgaag taacagacct
     1321 gcaataagtc caatacctcc accagcccat acatcaggtg accccagttc aggagggaga
     1381 tttaccatta aatttccttg tgagacgccc tcaaccgcat tctgacacaa aaagtacctg
     1441 accttatgag ttgacctgaa gaggccattt aactcgttac agagcgattg aagaggtctg
     1501 tgtgcaatgc aataaccatg tgaccattct catagccacg cctttatatt ttactctccc
     1561 cataaaccca ttataccaca gatgttaata aaatgttaaa aaatacttga cattagcctt
     1621 tgaaaatgct aggattgtat atatgagtta acagagaatt aacaattata agggccagct
     1681 tatgaatgaa cgacagagaa tttcaaaagt ttcttgaaga atccaagaga cgcaacagac
     1741 acaacggtta cagctacaca aacaacccca ccagctacga agtcccagcc ttttccaaaa
     1801 ccgaacgtaa aaacattgaa gcggtcattc gtagcattac gccacgagac agatttatgt
     1861 cagtacgcaa aacaacaaag aataacttga aacctttctt gatgagtttt gacagctacg
     1921 agcaactgcc agcaaaaatt gaagatttaa ttatcggtac ttgtcgcagt tttggaaggg
     1981 ataactatca ccggaaagtt ttctatttac tacgaaatat tgacaagatt agctcaagca
     2041 caataacaaa ttatctacag cgtcaagcaa ccagacttag ctacgagcta ccatctgata
     2101 aatattgcgc taatttaact accatttgca tgaaagttat cgagacaatc aatcatcatg
     2161 tggaagtggg caatattagc cttacaacca gtgaaccaga ttttgaattt gacccctata
     2221 ttttggaggg attttaaaat gaataaacac actttgaccc gtatttcaga agaagctttt
     2281 cacgagctta cccgaattaa acagacaact aacctgcctc atcaaactat tatgcagctt
     2341 gcaattgcta aagaggtcac tgagtcggat ttactgaaat atccagtttc taaaggtcgc
     2401 agacacatca gaattactaa gaacgctctg gctactctga aggaactgaa caactttaaa
     2461 attgatattg cccgactatt gagcatcaag attcacaagc tatcgatggg ggtttaatgc
     2521 tatgcattac ctttgaatac cacacagata aaatgattcg ctacatctcc gacctcctga
     2581 ttaaaggaaa cggattcggt gacatccata actcaaaaga tattttcatt aaagccattg
     2641 gcccaaatga aacactgaaa acagcagtta aaccggaatg gtttgaacgt cataagattg
     2701 aattaggtta ttggggtgag gaggttttat gaaaccttgg catgaagatg ttagaagata
     2761 ttttgcagaa caccttatct atgatgaatc cagtgattcc ctgtgctggt ctgatgggga
     2821 gtcagtcacg ataaataccg acgactacgg caacaaaaca tttaatatag gtcgatacac
     2881 attctatgtg aaatatgttg tctggttcct ttatcatggc tatcaatcca acaagcaaat
     2941 tatccatcga aatggtaaca gagccgatac cagaccaaaa aatcttatgc aagtccgaga
     3001 ctttaagaga aattagaaaa atagcttctc agacgctctc aatcgcattc taacgcaaaa
     3061 aactatctta ttttagggtg ggtaatacca ctgtacaggc tacccttgaa aaagactgtt
     3121 tagctcgtta cagagcgatt gaggaggtct ggtaaaaagg agattttagg aaatgaacaa
     3181 ttacccaatg acagtactaa ttgaccaaga aactatgaat tggttgattg acctgaatag
     3241 gtcaggattt aatatctatt tcatgcattg ttttgaagca tcaacgtata ttgacaagca
     3301 tacaatcaac gaaattaaac ttggtgactt tgttcacaaa ctggaagtaa cagacccaga
     3361 ctacaagaga tttatcactg aagagtgcaa tcgacttgat ttaagtcctg agcaattatt
     3421 gaagctgaat atattcttga cctctcagag tgaattaaac ccttacagcg aggtgatgta
     3481 atgacactcg ttattttatg gacagcttta ggtttattat gtggttgcct gattgctagc
     3541 gaagctaaca gaaattgatg ttatcaatct gttaaaagct gttgacaaat aaccttgagc
     3601 atgacataat tgtatatatg gagagagcaa ttctccttga attaaaatat taatcatctc
     3661 tacatcgatg atagttattc tgagttggtg aggtatgttc tataccgaac attctgaaag
     3721 gtgcaatgct tttcgctaca tgacctctta tataacctga gaggtcatct cgaaaagtct
     3781 ttgttcattc attgaatcct ttagatgttc tttaatttct tcaccttagc cctttatagc
     3841 gttttagtta tctagggctt tttcagcttt taaaaagcat acttggcaaa ccgcctttta
     3901 agtctcttct aagtttgtat ggctgtcagg tgtgcttttt aaaaactgaa tacctactta
     3961 gaagaggaaa atgaaatgga aaatctatat attaacggta aaaagattga tagcttcggt
     4021 gactggtcac cacagactga acatccagct atcgctattc agcgcaaaga gcacgatgaa
     4081 agaattgcac ttgagcaaga gttaaagatg acaccaaagg aaattacctt tgtgtctcct
     4141 gagcctcaag agatgcctga tgtatgcaag agtgaagcat tgcttgagct tgaaaagaaa
     4201 tatttcccag ctttaaaagc acagcgtctt agattagatg acgcttacag caaagttacc
     4261 ctgatgcaat cagctattga accgtcagaa tttgagattc aggatgaatt gtcacagaaa
     4321 ccatttgttt attaccaata tgcagacaat gatggttttg gtacttttcc tgagaatatc
     4381 cccgacgtga ttaataatct tcctgatgga tatcgaattg tgaagatggt taaagcatca
     4441 agaggtgctg gccagtttgt ttacatgagt aataagagcg atgaagaact cgctgaactt
     4501 gccagacaga acatcttggc atcacgtaac aagcaacttg aaaatgttaa gttgtatctc
     4561 tcaagagagc ttcaggctat gaaagagctt gttagtgaat atgagtcgca gaagaagatt
     4621 gctatgcaag ctgatattga gcagttagtg aagatttcaa cgaagtacgc aaaggtgctt
     4681 tgattacgaa ttaagaggtg attaatgttt gaagaacgtt tagagactat tcggtctgtt
     4741 tgtgaaaact taaagttaca aactaaacca gccttaagaa ttaaaagcaa acgtcaagtt
     4801 atcacctcac acaaaccaaa aacacgcaag attccaaagt ggtgtattga ccgtattcct
     4861 tctgatgctc aaatcatagg tgaaacggaa ttacattatc ttgtcagaca ttaaaaaaga
     4921 attggttggg tatatgagat aggttctatc gaatctataa tacggggaga gattatcgag
     4981 tgatatcggt agtttctccc ttttttgtat gaggtgaaaa aatatgaatg gcacaattta
     5041 aagttgatga ttttctgatt gagctaggtt ttaactctca gagggtttta aaaggtctgg
     5101 aaaaagccga gaaacaaacg atgcaggtag catcacgaat tgagaagcga ttgaataaag
     5161 cttttaaagt taaccctacg cctttaaatg attctctcaa ggtcatggaa agaaatgtcg
     5221 ataagactgt ttccaagatt gagcagcgtc ttaaaaacac taaagcgttt aagattaaaa
     5281 ctgagattga ggatacttta aagccactta gacaacaaag acagccacga attagcggca
     5341 acagagcaat cacagcagct tattcagcta atatgtctaa gctaaaaggc tttgacccta
     5401 ttttgcagaa atacatcaaa tctcagtttt acgggctatc tgctaaagct gggtcaatgg
     5461 attcttcaaa attcaatgag aagttagctc agttaaacgc ttcagtcaga gaagcaattg
     5521 ctaaagccag aggtcataca tccaccagca ttaacaatac aagcgacgca tttaataatc
     5581 ttgcaagcaa tgcaattaag ttatctggtg cattttactc tgtgatgggt gctcttaacg
     5641 cttacaaggc cattatgaat gcaggtctta aacgagattc cgcacagaga gccgctaagt
     5701 ttgtgcttgg tgataaagcc tctgaggctg aaacatttat caggggactg gctgacaaga
     5761 caggtcttaa tatctctgaa gggctatcaa gctacgctaa gtttgcagca ggtgctcagg
     5821 gttctatgtc acaggaacag acacaggagc tatttggtaa tgcaacggct atgagtcgct
     5881 taatggggct ttctaacgac gagctaaacg gcattctgaa agcatttgag cagatggcga
     5941 gcaagggcaa gattcaagct gaagagcttc gtgggcagtt aggtgaccgt atggctggtg
     6001 catttaagct attcgctgaa gcattgggta tgactgctac agagcttgat aaagcgatga
     6061 aagacgggaa gattttaagt agtgatactc tccctaaagt cgctaaacag atgggcttga
     6121 tgattgataa agcgggtggt tgggctgaag tcgctaagag cactcagaca gctttaggta
     6181 agttagcaaa caactgggat gatacgatgg ttaaaatctt ctcaggttca caggatgagt
     6241 taaacggctt cttatcccgc ctgagtagcc tgcttagtga aatgggtatg agttcttcta
     6301 tcgctggtga tgctatcggt ggacttatcg acatgcttaa agctggtgta gatgacatcc
     6361 gtatctttaa caatcacctt gaaggctgga ttttacagac taagaagctc tattacagcc
     6421 ttgatgacac taaacgaaaa ctacttgatg aagtcggtga cggttttatc aattttgtca
     6481 aaggtcttgc tatagcgtta tctgcaaaga cactattcag tgccactaca ggggtgatga
     6541 acctgacaag agcaatcaca acgttaggta caagagctaa tcaggtggct ggtcaggtag
     6601 ctactggtaa aggtggtgga aaactgaaag gtgtagctgg tggtgtaggt agtgctcttg
     6661 caattggtta tgctgatgac ggttatgaaa cagcgttagc attagcttca atgattccac
     6721 aaatcagagg cgtcactctt ggtttatatg cattgaagaa agcactggat tttatgaatc
     6781 aggaagtagt caaaaacgct aatatgcatc cgtctggggt aggtgtgggt agtgacttta
     6841 atcctgtgta ttctggtgac cctaataaac cgaatatgat taacgaagga tggggtagaa
     6901 tttttagttc aatgggtgaa ttgtttaaca atgccacaat gaatattgca agattcaatc
     6961 atccagaatt aattaccatg aaacaagatg catcattaac gagtgcagat attcagggtc
     7021 tgagggatga aatctctgct ttaagtaagc gtattcagga gcctgtgaaa gtttcacttg
     7081 gtggtgaagt tgccattaaa ccagatgaaa ccagctttat gacctttagc agtaatatct
     7141 acgaccaata tgcagaagct acgttattaa gttcatcatt cccagaagat gattgattta
     7201 acggtgttct acgtgaataa agcaaagtta atacccgcac tgaaaaatag aatttacgta
     7261 gctcacgctt aacagataaa acacttaaat atcaaaggct tggttaaaaa tgattaaaat
     7321 gcactaagtc tttgaaatca aaggggaatt taatgattta ctctaaccaa atcaaatata
     7381 gtggcaaaga cggcatctat ttccacttaa gagataatgt agatgccttc ttaaccttat
     7441 cagcaactga gaacatggaa tttgacagcc ctatgcaggt aactacacag aacatgcaat
     7501 cagggcaaac cgtcacagat aatgtgcaaa gagcacccag aacaatcact attagtggtg
     7561 tcgttgtagt tggctatgaa ggaagcttgt tattaactcg tcagggtcaa ttagtagaaa
     7621 acttcatcga cactctggaa agctggcgtg accagaagca aattatttcg gtcatctgta
     7681 aagatggtat taaaattgat gattccatta ttacaagctt taaagcctct aaagatgttg
     7741 gtatttcgaa tggtctgaga atccagttaa cttttcagga gattaacttc aaagccattg
     7801 tgggtcagac tgatatttcg gctgcgacag gaaaaaccgc taccacaaat gacggtggtg
     7861 tgaccagtaa gaagaatgca ggtaacgtga ccactgaacg caataacgcg cctatgctat
     7921 cgtgcaaaga gttaatgaat attagcgatg tatccggtta cgctccagct atcagaaatg
     7981 atattctcga tgctcagatt aaatgtaata aggcttggaa gctggactat tcagacttaa
     8041 cagaagctga aaaaaccaac gtcaccaatg ctaacagtaa cgctggtcga gtagcttcaa
     8101 agcttaaaaa gtactcagta aatcctaata agagatattg atagaagggg gcatagcccc
     8161 ctgattgatt gctacagttg gaaagtgtca atattcaaaa gatttttctc agcaatttca
     8221 aagattgatg ttccatcatt tagcggtagc gggtctgtag gtatggttat ttcagtgtcg
     8281 ttctgtagcg ttacagttac cgaatcctta cccaatgtaa gcccttttag atgtttggac
     8341 aaaatcaact gacactcgac acgacccgct ttagttgtca ggtcaatatc tttaagctgg
     8401 ggtagttcct tatcagtgag tgaagcctta cgcctcatgt tatcaatcgt ggtctgtagc
     8461 tctgtgcgct tctggttaag tcccttcacc tgtgttttca actctgtaat atcatccatc
     8521 tcaagaagta gggcggtagc tttcttgatt ctggctgtga caatttcaac ctcatgctgt
     8581 aacgctgcaa tcttcctctc tgattctcca tcattcccca cgtcaatcat ccccagaaaa
     8641 ttatcaatca tccacttatc aaacggttta cgcttcaacg gtggaacatc acagcgaccg
     8701 tcaagatggt tattgcaccg ataaacaccc tgatacttat tcttagtccc tgtagggtga
     8761 acactagccc cacactgaca ccggaataat cccttgaaga tgttaatcgt taatgggtta
     8821 tcac
//
LOCUS       DAZFVO020000183         8820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFVO020000183 DAZFVO020000000
VERSION     DAZFVO020000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.184"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..661)
                     /gene="upp"
                     /locus_tag="ACHKNB_RS21645"
                     /old_locus_tag="pgaptmp_004329"
     CDS             complement(35..661)
                     /gene="upp"
                     /locus_tag="ACHKNB_RS21645"
                     /old_locus_tag="pgaptmp_004329"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGU4197724.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            986..2023
                     /gene="purM"
                     /locus_tag="ACHKNB_RS21650"
                     /old_locus_tag="pgaptmp_004330"
     CDS             986..2023
                     /gene="purM"
                     /locus_tag="ACHKNB_RS21650"
                     /old_locus_tag="pgaptmp_004330"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGU4197725.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2023..2661
                     /gene="purN"
                     /locus_tag="ACHKNB_RS21655"
                     /old_locus_tag="pgaptmp_004331"
     CDS             2023..2661
                     /gene="purN"
                     /locus_tag="ACHKNB_RS21655"
                     /old_locus_tag="pgaptmp_004331"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGU4197726.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2831..4897
                     /gene="ppk1"
                     /locus_tag="ACHKNB_RS21660"
                     /old_locus_tag="pgaptmp_004332"
     CDS             2831..4897
                     /gene="ppk1"
                     /locus_tag="ACHKNB_RS21660"
                     /old_locus_tag="pgaptmp_004332"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HGU4197727.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4902..6443
                     /gene="ppx"
                     /locus_tag="ACHKNB_RS21665"
                     /old_locus_tag="pgaptmp_004333"
     CDS             4902..6443
                     /gene="ppx"
                     /locus_tag="ACHKNB_RS21665"
                     /old_locus_tag="pgaptmp_004333"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4197728.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6482..8725)
                     /gene="pdeF"
                     /locus_tag="ACHKNB_RS21670"
                     /old_locus_tag="pgaptmp_004334"
     CDS             complement(6482..8725)
                     /gene="pdeF"
                     /locus_tag="ACHKNB_RS21670"
                     /old_locus_tag="pgaptmp_004334"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HGU4197729.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         2272 a         2173 c         2194 g         2181 t
ORIGIN      
        1 gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag attttgtcac
       61 cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctga tcaatcgatg
      121 cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct tctggcgcag
      181 cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg tcgatggtcg
      241 cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata cgctcatcga
      301 tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca ttacggtaca
      361 taccgacaac gctgatgcgc gcgctcggaa cgttttccag cacaccgtcc atcataccaa
      421 gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg tcgatttcta
      481 ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg gtcgcttcgt
      541 aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg ctgatatctt
      601 gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc acgatcttca
      661 tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac ctcctttctt
      721 caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt gagataaagt
      781 tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta acctttaccc
      841 tggcacaagt cttctttcgc cgcgtgcctg ggcaaaagac gtgcaaaaag gttgtgtaaa
      901 gcagtctcgc aaacgtttgc tttccctgtt agaattgcgc cgaattttat ttttctaccg
      961 caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct acaaagatgc
     1021 cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg tagtgaagaa
     1081 aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt gtgcattgcc
     1141 gcaaaaatat cgtgaacctg tgctggtttc cggtactgac ggcgtaggta ccaagctgcg
     1201 tctggcgatg gacttaaaac gtcacgacac cattggtatt gatctggtcg ccatgtgcgt
     1261 taatgacctg gtggtgcaag gtgcagagcc gctgtttttc ctcgactatt acgcaaccgg
     1321 aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag gatgcctgca
     1381 atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt atcacggtga
     1441 ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa tcatcgacgg
     1501 ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc cgcactcgaa
     1561 cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc aaaccaccga
     1621 acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt acgtgaagtc
     1681 agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga ccggcggcgg
     1741 cttctgggaa aacattccac gcgtattgcc agataatact caggcagtga ttgatgaatc
     1801 ttcctggcag tggccggaag tgttcaactg gctgcaaacg gcaggtaacg ttgagcgcca
     1861 tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc ctgctccgga
     1921 agtggacaaa gccctcgccc tgctaaactc caacggtgaa aacgcgtgga aaatcggtat
     1981 catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat tgtggtgctt
     2041 atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac taacaaaatt
     2101 aaaggcaccg tacgggcagt tttcagcaat aaggccgacg cgttcggcct tgaacgcgcc
     2161 cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag tcgtgaagcc
     2221 tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt cgtgctggct
     2281 ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg tttgctgaac
     2341 attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca ggcgctggaa
     2401 aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact ggacggtggc
     2461 ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga tgacatcacc
     2521 gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg gtttgccgat
     2581 ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct gccgccgcag
     2641 ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc gctggcgttt
     2701 ttcaattcac ctgtaaatcg caagctccag cagttttttt ccccttttct ggcatagttg
     2761 gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat aaaacggagt
     2821 aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt ggttatcgtt
     2881 caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg aaaggatgcg
     2941 tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct tcgctgaact
     3001 gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc gccatttact
     3061 gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc tctacaacga
     3121 gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc agctctccgt
     3181 caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc acattacgcc
     3241 gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt acacctatct
     3301 ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga tcccatcaga
     3361 taaagtgccg cgctttgtga atttaccgcc agaagcgccg cgtcgacgca agccgatgat
     3421 tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct tctttgatta
     3481 tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg atttagtgca
     3541 tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc gtttaactgc
     3601 tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg aagtgttacg
     3661 cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt atcataattt
     3721 taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca aaccactgcc
     3781 gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg atgccattcg
     3841 cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc tggaactgct
     3901 gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt accgcgtggc
     3961 gaaagattca cgcatcatcg actcgatgat ccacgccgca cataacggta agaaagtcac
     4021 cgtggtggtt gagttacagg cgcgtttcga cgaagaagct aacattcact gggcgaagcg
     4081 cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa ttcacgccaa
     4141 actgttcctg atttcacgaa aagaaaacgg tgaagtggtg cgttacgcac acatcgggac
     4201 cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcattgc tgaccgccga
     4261 tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc cataccgccc
     4321 ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt tatatgaaat
     4381 ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agcggtatca ccctgaagct
     4441 aaataacctt gtcgataaag gcctggttga tcgtctgtat gcggcctcca gctccggcgt
     4501 accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg aaggcattag
     4561 cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc gggtttatat
     4621 ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga tgacgcgcaa
     4681 tattgattat cgtattgaag tggcgacgcc gctgctcgat ccgcgcctga agcagcgggt
     4741 gctggacatc atcgacatat tgttcagcga tacggtcaaa gcacgttata tcgataaaga
     4801 actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc agttggcgat
     4861 ttacgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata cacgataaat
     4921 cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt cacatggtca
     4981 tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag cgggtgcatc
     5041 tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg cgcggtttga
     5101 actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc gtctgtatag
     5161 tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa cgcgcggaaa
     5221 aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt ctgattttta
     5281 tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat attggcggcg
     5341 gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa agccgccgga
     5401 tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat aaagagaatt
     5461 ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg caattccgta
     5521 ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc catgaagtgt
     5581 taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa aaactggtaa
     5641 aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt tccgaagagc
     5701 ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat gctttagcca
     5761 tccgtgaact gcgcctttct gacggggctt tacgcgaagg cgtactgtat gaaatggaag
     5821 gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc aaccagtatc
     5881 acatcgacag cgagcaggcc cgacgggtgc tggataccac tatgcaaatg tacgaacagt
     5941 ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg cgatgggccg
     6001 ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc cactccgctt
     6061 atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg atgatggcga
     6121 cactggtgcg ctatcaccgt aaagcgatta agctcgacga tctgccgcgc tttaccttgt
     6181 ttaagaagaa acagttcctg ccactgatac agctattgcg ccttggcgta ttactaaaca
     6241 atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat gacagtcact
     6301 ggacactgcg tttcccgcat gactggttta gtcagaatgc gctggtactg cttgatctgg
     6361 aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt gaagaagaaa
     6421 gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg gcccgcatta
     6481 ttcaggcact ttcgcgaatg ggttcgattt cattcagcgt atcaattaac ggttgcggct
     6541 taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc tcgcggatct
     6601 cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc aggtggcaaa
     6661 tcgatgccac tatctgataa tccagactat ttgacacaat attgcggata aaactgccgt
     6721 caattttaag cagatcggca ttcacatttt taagccgcgc atagctggcg tagccggtgc
     6781 cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag gtgatttgcg
     6841 cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt tgccacgctt
     6901 caatctgata tttatccagc aactgactga cttcaacagg aaaacgagcc tgacataccg
     6961 aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg ttttcagcca
     7021 taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa ccaaattcgt
     7081 gcgcgaccgg caagaagcta tcggggccga tcagttcatc attctcacct ttcatgcgca
     7141 gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc atcaggaaaa
     7201 aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta tctttcaatt
     7261 cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg gtcacgatgg
     7321 aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc actggcgagc
     7381 gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catgccatcc caaaagaaac
     7441 gaaattgctt gagatggcta tccagtgcgg taatgcgctc ctggtgcgat tctgtattca
     7501 gccgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt tccagcaagg
     7561 gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg ccatagttct
     7621 taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag ggggcatcac
     7681 gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc acatacgcaa
     7741 gccgctgaat gcgtctgact accgctcgct gacgagttgc cagtacagcc atataattga
     7801 caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg gtatagccag
     7861 gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg accgcccaga
     7921 gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg ggcagcaata
     7981 atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc atgcataaca
     8041 gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg gtgactttgg
     8101 catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga tttcgcacta
     8161 cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt aaggcctgat
     8221 aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct ctgctcgcca
     8281 gcaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca ggaaacacta
     8341 tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg ttgttacgcc
     8401 ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata aaattagccg
     8461 ttaatgagag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa atccctaagg
     8521 tgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc agtgccaaag
     8581 gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta atgtgggcga
     8641 aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta tcacgtattt
     8701 ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat cctgaaggag
     8761 gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca ctatagagag
//
LOCUS       DAZFVO020000184         8791 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFVO020000184 DAZFVO020000000
VERSION     DAZFVO020000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.185"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            134..2182
                     /gene="ireA"
                     /locus_tag="ACHKNB_RS21675"
                     /old_locus_tag="pgaptmp_004335"
     CDS             134..2182
                     /gene="ireA"
                     /locus_tag="ACHKNB_RS21675"
                     /old_locus_tag="pgaptmp_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HGU4197730.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDRGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVNDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDMLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
     gene            complement(2428..2859)
                     /locus_tag="ACHKNB_RS21680"
                     /old_locus_tag="pgaptmp_004336"
     CDS             complement(2428..2859)
                     /locus_tag="ACHKNB_RS21680"
                     /old_locus_tag="pgaptmp_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HGU4197731.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(2927..5638)
                     /locus_tag="ACHKNB_RS21685"
                     /old_locus_tag="pgaptmp_004337"
     CDS             complement(2927..5638)
                     /locus_tag="ACHKNB_RS21685"
                     /old_locus_tag="pgaptmp_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HGU4197732.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(5650..5982)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS21690"
                     /old_locus_tag="pgaptmp_004338"
     CDS             complement(5650..5982)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS21690"
                     /old_locus_tag="pgaptmp_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4197733.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            6215..6550
                     /locus_tag="ACHKNB_RS21695"
                     /old_locus_tag="pgaptmp_004339"
     CDS             6215..6550
                     /locus_tag="ACHKNB_RS21695"
                     /old_locus_tag="pgaptmp_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HGU4197734.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKTSRRSSSKTAPKKTDSAR
                     KDGIDMNNN"
     gene            complement(6635..7483)
                     /locus_tag="ACHKNB_RS21700"
                     /old_locus_tag="pgaptmp_004340"
     CDS             complement(6635..7483)
                     /locus_tag="ACHKNB_RS21700"
                     /old_locus_tag="pgaptmp_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4197735.1"
                     /translation="MNQTLPTADLNTDGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            7832..8028
                     /locus_tag="ACHKNB_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /pseudo
     CDS             7832..8028
                     /locus_tag="ACHKNB_RS21705"
                     /old_locus_tag="pgaptmp_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8062..8313
                     /locus_tag="ACHKNB_RS21710"
                     /old_locus_tag="pgaptmp_004342"
     CDS             8062..8313
                     /locus_tag="ACHKNB_RS21710"
                     /old_locus_tag="pgaptmp_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4197736.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(8344..8550)
                     /locus_tag="ACHKNB_RS21715"
                     /old_locus_tag="pgaptmp_004343"
     CDS             complement(8344..8550)
                     /locus_tag="ACHKNB_RS21715"
                     /old_locus_tag="pgaptmp_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197737.1"
                     /translation="MTIAERLIQKGFDEGFKEGFKQGFKQGALEVAREIACRLRDIGW
                     PPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(8612..>8791)
                     /locus_tag="ACHKNB_RS21720"
                     /old_locus_tag="pgaptmp_004344"
     CDS             complement(8612..>8791)
                     /locus_tag="ACHKNB_RS21720"
                     /old_locus_tag="pgaptmp_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4197738.1"
                     /translation="ERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQIK
                     QATGLSEAELKKVIH"
BASE COUNT         2306 a         2226 c         1975 g         2284 t
ORIGIN      
        1 ggtatgttca ggaaattatt cgaaaatgtt aactgaatga gcgaaatgtg atttaccttc
       61 ttttctgttc attgtaaatt ccctctttgc taacgcaaat cattatcatt acgcctttgg
      121 caaagggaaa tttatgaaga ataaatatat cattgctccg ggcattgccg tgatgtgttc
      181 tgcagttata tcatcaggtt atgccagttc tgataaaaaa gaagatacgc ttgttgttac
      241 tgcctccggg ttcactcagc agctcagaaa tgccccggcc agtgtctcag tcattacttc
      301 agaacaactg caaaaaaaac cggtttcaga tctggtcgat gcagtaaaag atgttgaagg
      361 gattagtatc actggtggga atgaaaaacc ggatatcagt atacgtggtc taagtggcga
      421 ttacacgctg attctggtcg atggacgacg tcagagcggt cgggaatcca gaccaaatgg
      481 cagcggcggt tttgaagccg gatttatccc tcctgtggaa gcaattgaac gcattgaagt
      541 gatccgtggc cctatgtctt ccctgtatgg ttctgatgcc atcggagggg tcattaatat
      601 cataaccaaa ccagttaata accaaacatg ggatggcgta cttggacttg gggggattat
      661 tcaggaacat gggaaatttg gtaactcaac cacaaatgac ttctatctgt caggcccatt
      721 ggttaaggat aaacttggtc ttcagctata tggaggaatg aactatcgca aggaagatag
      781 tatctctcag ggaacaccgg caaaagataa taagaatata acggcaacgc tccagtttac
      841 tccgactgaa agccagaagt ttgtttttga atatggaaaa aataaccagg tgcatacatt
      901 aacacctggt gagtctctcg atgcctggac tatgcgggga aatcttaaac aaccaaacag
      961 taaaagagaa actcataatt cacgtagtca ctgggtagca gcatggaatg cccagggcga
     1021 aatactgcat cctgaaattg ctgtttatca ggagaaagtt attcgtgagg ttaaatcagg
     1081 taaaaaagat aaatataatc attgggatct taattacgag tcaagaaaac cggaaataac
     1141 caacacaatc atagatgcaa aagtgacggc atttctgccg gaaaatgtac tgaccatcgg
     1201 aggtcaattt cagcatgcag aactccgtga tgactcagcc acaggtaaaa aaacgacaga
     1261 aacacagtct gtttcagtta aacagaaagc tgtttttata gaaaatgaat atgctgcaac
     1321 ggattctctc gctttgactg gaggactgcg tctcgataat catgaaatct atggcagtta
     1381 ctggaatcca agattgtatg ctgtttataa cctgaccgat aatctcactc tcaaaggggg
     1441 gatcgcaaaa gcatttcggg ccccttcgat tcgtgaggtc agtcctggat ttggaacact
     1501 gacgcagggt ggcgcctcta ttatgtatgg taacagggac ctgaaaccgg agaccagtgt
     1561 aaccgaagag atcggtatta tttataataa tgaccgtggt ttctcagcaa gtgcgactct
     1621 gtttaataca gattttaaaa ataagctgac cagttacgat ataggcacaa aagatccggt
     1681 cactgggtta aacactttta tttatgataa tgtgggtgag gcaaatatca gaggtgtgga
     1741 gcttgcaact cagattcctg tgaatgataa atggcgtgta tctgcaaact atacattcac
     1801 tgactcccgt cgagaaagcg atgacgaaag tctcaatggc aagtcgctga aaggggaacc
     1861 tctggaaaga actcccagac atgcagccaa tgcaaaactg gaatgggatt acactcagga
     1921 tattacattt tattcatctc tgaattatac gggaaaacaa atctgggcag cacaaagaaa
     1981 tggtgctaag gttccccgcg ttcgtaatgg attcacatct atggatattg gtctaaatta
     2041 ccagattctg ccagacatgc tgattaattt tgccgttctt aacgtcacag acagaaagag
     2101 cgaggatatc gataccattg atggtaactg gcaggtcgat gaaggacgcc gttattgggc
     2161 taatgtaaga gtatccttct gagagtatat aaagataacc tgaggccgtg agcctcaggt
     2221 accagtgtag taatcagtaa cgtcagtttc caggttgtaa tgcttttaat cttgatttaa
     2281 ataagcttaa taaattacac ttgttaaaca tggatatttt taatttttga gtatgtcaac
     2341 ataacctgag gcaaagtacc tcaggttcca gtgtagtaat cagccaacct gacagtcttc
     2401 tgttagccct acagatatgt cgtggagcta accgcgcatg tcaaagccgg atttttgaaa
     2461 atctctataa acttccggat tgttgaaggc cgggaattta gctttatggg ctgctgactt
     2521 tatcgcttcg caataggctt tatcgccatt actggttgat atttttaacg ccgtgccatc
     2581 ccgggagaat tccatatgca acctgcattt tttccttttc catttttgcg gatcatcaag
     2641 tttagcatta attgcatccc tgattccccg tgcctgctcc ccccattcat cctgatcatc
     2701 ccaacgtcct gaactgcaac tacccagggc tgtagtttta tggcaatttg aaggatgtaa
     2761 tggcgcacat cccgccacaa agctgactaa aatagccaac ataacgattt tctttacccc
     2821 catatattgc tcctcaatcc atatagatct cagtaccata gctctgtcag agcaaagaaa
     2881 atcttaacta atacaagtta agatttttaa tatccactcc tcatccctac attccaggtg
     2941 atggtggcgt ccactcatta tcctgaactg gagcataaga tgcgccatgc cccccggata
     3001 tgcttttctg ttgcctgata tattcgtggt agtactgttc actacgctga cgatgtaaat
     3061 ccacatccgg gcggctgatt acaaagtgtc catttccggt tattccagct gcattatgcc
     3121 aggccaccat ttccgcaaat acatcattaa actgttgata tgagccttcc ggttcaaaac
     3181 ccattcctgg ctcacgatca aacaatacag gagcaatcag attcagctta tccaccagtt
     3241 catttctgtc ctgatgatgg tagaacacca cacggtcccc ataatccaca cagactaact
     3301 cgccattcct ccgatcacga aaacgcacac tgccatcttt ctgcagacgg gcttcgagaa
     3361 cgccagtgtc ttcataaagt ggagttcctc ccggttcaca gagaatcacg cagcggtcgg
     3421 cattcgttgt tcgtcgcttg tctttgcgac gatcacgata atcccagcca cgtaactgtg
     3481 ataatgcagc tctgtctccc tgtaccgcct gttgctccac ccactgccgg taggacaaag
     3541 gataccactt accctccgca atcagtgaca accgctcttc cttcacactc tccttcagcc
     3601 tgatcagcgc ctgcatacgc tgcacttccg caatatggta atgcagctta cgcaattgtg
     3661 gatcccggaa ctgcacacgg atatacgcct tgcgccgacg gcaggcagca tgaatttccc
     3721 gtaaccgttc tccgtaacgt aagtcgggtt tacgccagtg ctccttccat gcaagataac
     3781 gcgcgcgcaa atcttcccgc gcagctgcac gggcttcacg gcgttcacga cgcagtacag
     3841 gatcgcgccg gaagccgggc tccacttcat cgctggcagc aagttccgga ttgtagcgac
     3901 actcaggctt cacccgttca aatatatctg cacctgcaat ctcaaatggc cctgcctgcg
     3961 gctcagccct ggataacgtc aaatccgggt ggatactgct ggccttcact ggtgtgagct
     4021 catgattaaa cgcatccaca atcacaagac catgatgctg tttctgcaac ataagccctt
     4081 tacgcgcaaa catctcatgg cacgcctgcc agctgaaatt cccgcccgcg atcatatctt
     4141 caatcaacga ctcacactga acagtcgtgt acccccacag actttcctta tcagaaaaca
     4201 gttcgcgctt tattgctccc tgtggacctg tccccatccg catacgatac cattcgtaat
     4261 cactgacagc ctgagtacca agattacgtc cccggaatcg ttctccgggt ataacctgag
     4321 taaaaatatc cttcggtaca ggctgccatc caccatccag atcctgcata taatccagcg
     4381 tcattaacgg ccacacactg tctgcacgta ctggtgtctg ttgtttatca aatccatcat
     4441 gcagtaccag atgcccgtgc tggatcccca tccataatcc agcctttgcc agagttctgt
     4501 gcacatccag gcaactttcc agctcaccat ttctcgccat ttccggtaag cgttcggcaa
     4561 aatgacggca ggcgtactgt ttcagactct ccgtttctgc cactttttcc ggccggatat
     4621 caacgtttat ggcttccgga tcataacgac cgggtgtccc cacctgactg aaaatatctg
     4681 tcggtacagg ctgatattcc cccagtttcc gccccagttt ttcagctgtc catgacggtc
     4741 cgaacgaact gagtgcaaca ccttcccgcg ctctgtccca gccatccttc accaccagtt
     4801 ccccttcctg catcgtgata tacagaccat cactggccag ccgtttatgc aaagataacc
     4861 agtcctgtgc gggctcttca cgcagcccgg caatcgacat ctgagcaata tattccctga
     4921 acgtctgctt tttcccgcgt cgccaggcat tacggcgctc acgtaccagc gctgttttgc
     4981 gcacaatacg gttgccaggt gcatgcacaa agcagccatt atcaggcgcg aatccgtgct
     5041 taagttcaag ttcacgacat gcacggctga gtttttcctg gctccaggaa agccagttaa
     5101 tataaccagt ttcaggatgt acacgattca cggcaacatg cacatgcaga ttatccgtgt
     5161 ccgtatgaac ggcagccacg tactgatgtt ttgagagttc aagagatttc agtgtgtgac
     5221 gcacacagtc aaaaagctgc tcaggacgcg ggctttcatg cgcaggccag gaaagaatgt
     5281 agtggaaaac ggggtctgta tcattacggg aaaaaacagc tttgtccgcc ttgtactgca
     5341 tctcttcagc agctgtttcc agtgaattgc agttatggaa acaggttacg ccatagaaat
     5401 ttacccactg actgccgtct ttcatcacat caatcaggga gacaaatttt tcgttacgca
     5461 ggcgtgttgc ataatcaaca agccgtctga aacggttgcg gtgagggaca tcactcttga
     5521 tttcgctgtc ttctgtgagc tcattttcct gcacatcatc ccgcacagac gtgtacgcaa
     5581 tcaggtcttc aaaggaagac ctgccgtcac gtcttttgtg cggaataatg gcattcatca
     5641 gcagttatct cagcgtccca ttaagtctct tcgcagagca ttgacatatt cagtaatggc
     5701 aacaagaacc tctgcatact ccttatcacc agtgcgtttt ccctccacaa acagatgctt
     5761 ctgaaggcgg ccaagacgca taatctcatc aatcatacgg tcgtcagtac ggcagttaac
     5821 caccttatcc agtgaagagc ttcgtataaa tgttgaaacc gtctgtccac ttgactccgc
     5881 tttttctctt acgatttcag cctcatcctc cgtgaatctc gccgtgaatt ttacaattct
     5941 cttcctgttc tcactgcccg ttcgggtttt cttctcactc atggtctgct ccttaccggg
     6001 gtgtcggggc aaagccctga ccaggtgggg aatgtctgag tgcgcgtgcg cggtccgaca
     6061 ttcccacatc ctgtcccgtt tttcatgcca ttatagcctg ttatttttgt gcatcatagt
     6121 cttatgcaga cggcagaggc agggcgcagc cctgccagga ggcagttttt gatgataaaa
     6181 tcagacttca acatgaaacg gagagaaatt gccaatggct tctaaaaaat tttactcaga
     6241 tgatgacatc cagctggcaa aagctgcttt atcagaattg ccggacctca ccgctcaacg
     6301 gaaaacattg cgtgattttc ttgatgccat tcgtgatgac atcattgtcc tggtaaggac
     6361 aaagggttat acactggcag atgttcgtga cacgctcaaa aatgcagggt atgaagtagg
     6421 agagaaggca cttcgtgaca ttattcggga agcagaatcg aaaaaaacat cccgccgttc
     6481 ttcctctaaa acagcaccta aaaagacaga ttcagccagg aaagatggca tcgatatgaa
     6541 caacaattga tgtgacaatg tcacatcgta ttctgagatt atgatgatta cagagaaaaa
     6601 gccctggcat aaataccagg gcttttctgt ttaatcagac tcgtggcggc aatgcaccag
     6661 gataatgccg tgcaataata tcattcagtt tttcaacaag gtcaggacgc ttaaagacaa
     6721 tgtgtacggt tcctttctgg tagccacgca ccgtaaaata ttccccttct aacagcttac
     6781 cgacattgtc tccgttcccg agaaattcac catactgcgc accgatgctg ctccggaagt
     6841 caggaacgtt gcgcccctcc agcagataaa acggacgtac caggtcatcc agtgcctccc
     6901 gtccgctgca gtccagcgtc acacgcccgt tactccagcg gtacagcagg ttttcaatga
     6961 tgattttctt gcccagacgg cagggattat tggttctgta atcccatgac aatttccgcc
     7021 agacatcaat cagtccctgt tcaaatgtct gcatcttact ggcattcaga tggcgaaaag
     7081 ttgccagtac attgtccagg gttatttcag gacaggtgtc gctcatcagc tggctgttcc
     7141 actcatcacg ctgacatgaa ctcatgaagg tgtacatccc cgtatccgtc atcaggcggt
     7201 cccagatttt ccggtcggca agacgggtga tattcctttt cgcttcttcc ggtttatcca
     7261 tgcatcgaac ggagtcagtc acgcagtctt caaacccgta gagaaaaatg tcaccggaag
     7321 catcgagcag aatcctgcgt gctgtcgcca ggcattccat cgcctgcatg aacagtgaaa
     7381 taccttcgtt acgctgcgcg atgatgcggt cgatagccac agacggaata acatctgtcg
     7441 tgccgtcagt attcaggtca gcagtgggta aagtctgatt catcacattg tctcctcatc
     7501 gtcacgactg ccgggtcagc ctggtcattt cccggaggag cgaagctcct tctgccgtcc
     7561 tggtcttcgt ggatttcccc gcagtggacc tttccgacgg gggacgctca ccacgtcacg
     7621 gggcagttgt ccgggggcag acgaaaaagt ttttgacgcc tgcggcaaaa agttttgtgg
     7681 ctggtacatc tctcccctgg acgactgaaa cgggatgtgg tcgtgtatgt acccccggaa
     7741 agtccgtctg cggagaacag gccgcagtgc ggcggcagac aggcagagac agctgcgcca
     7801 tcaggcgcag tgaccttaca caccgtcagg tgtgcaggga gcgccgcatc cggcgcgact
     7861 gaagaaaaac gatggcacaa caatggcatg tggtggtcac tgacgcacca ctcccgcaca
     7921 ggtgaaaaca gagaaaaccg cacgacatct acaataaaaa aagtgaaatt tcttcacttt
     7981 ttcacactta tttatcgcgc tgatttggtg attatgatga taaaataaag tgaagttttt
     8041 tcattttttc aaaggtgttt tatgaccggc tgggaactgc gaatctggcg aaaaagcatg
     8101 ctctggtcac gcgaaaaagc ggccagagaa tttggcgtga cgcagagaac atggcacgcc
     8161 tgggaaaatg cggaacaggt ggacgtcacc gtctggagga ccacccaggc actttcagtc
     8221 cgtgacctgc tgcctcacat gcaggggatg cgcaaggcag atatcatcag gcgtctggaa
     8281 aatgaactgg gggaaacggc agggaatgtg tgagcgtgat tcacagcctg gctgaatgcc
     8341 aggctactgc tcatcaggaa acagcttttt cagttcttca ccggaaagtc ccgtgacctc
     8401 ctgaatccgt tccggcggcc agcctatatc ccgaagccgg caggctattt cccgcgccac
     8461 ttcaagagct ccttgtttaa aaccttgttt aaaaccttct ttaaaaccct cgtcgaaacc
     8521 tttctgtata agacgttctg caatcgtcat gctgtttgct ctgctcctgc tggcaaaaag
     8581 cccggcattt tgccgggctg tgatgctctc atcagtgaat gacttttttc agttcagctt
     8641 cggaaagtcc ggtggcctgc ttaatctgct caggtgtcat ccccattttc tgaagctgac
     8701 gcgcaatggc cagggctttt tcaagcgcac ctttctgctc aagacgttct gcgattgtca
     8761 tcagtttatc ctcatgctgc ggcaggcgct c
//
LOCUS       DAZFVO020000185         8663 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFVO020000185 DAZFVO020000000
VERSION     DAZFVO020000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.186"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(307..570)
                     /gene="rpsT"
                     /locus_tag="ACHKNB_RS21725"
                     /old_locus_tag="pgaptmp_004345"
     CDS             complement(307..570)
                     /gene="rpsT"
                     /locus_tag="ACHKNB_RS21725"
                     /old_locus_tag="pgaptmp_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGU4197739.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            673..891
                     /locus_tag="ACHKNB_RS21730"
                     /old_locus_tag="pgaptmp_004346"
     CDS             673..891
                     /locus_tag="ACHKNB_RS21730"
                     /old_locus_tag="pgaptmp_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HGU4197740.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            899..1840
                     /gene="ribF"
                     /locus_tag="ACHKNB_RS21735"
                     /old_locus_tag="pgaptmp_004347"
     CDS             899..1840
                     /gene="ribF"
                     /locus_tag="ACHKNB_RS21735"
                     /old_locus_tag="pgaptmp_004347"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGU4197741.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1883..4699
                     /gene="ileS"
                     /locus_tag="ACHKNB_RS21740"
                     /old_locus_tag="pgaptmp_004348"
     CDS             1883..4699
                     /gene="ileS"
                     /locus_tag="ACHKNB_RS21740"
                     /old_locus_tag="pgaptmp_004348"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGU4197742.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4699..5193
                     /gene="lspA"
                     /locus_tag="ACHKNB_RS21745"
                     /old_locus_tag="pgaptmp_004349"
     CDS             4699..5193
                     /gene="lspA"
                     /locus_tag="ACHKNB_RS21745"
                     /old_locus_tag="pgaptmp_004349"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGU4197743.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5281..5730
                     /gene="fkpB"
                     /locus_tag="ACHKNB_RS21750"
                     /old_locus_tag="pgaptmp_004350"
     CDS             5281..5730
                     /gene="fkpB"
                     /locus_tag="ACHKNB_RS21750"
                     /old_locus_tag="pgaptmp_004350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HGU4197744.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5732..6682
                     /gene="ispH"
                     /locus_tag="ACHKNB_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /gene_synonym="lytB"
     CDS             5732..6682
                     /gene="ispH"
                     /locus_tag="ACHKNB_RS21755"
                     /old_locus_tag="pgaptmp_004351"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HGU4197745.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6748..7662
                     /gene="rihC"
                     /locus_tag="ACHKNB_RS21760"
                     /old_locus_tag="pgaptmp_004352"
     CDS             6748..7662
                     /gene="rihC"
                     /locus_tag="ACHKNB_RS21760"
                     /old_locus_tag="pgaptmp_004352"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HGU4197746.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     ALLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7829..8650
                     /gene="dapB"
                     /locus_tag="ACHKNB_RS21765"
                     /old_locus_tag="pgaptmp_004353"
     CDS             7829..8650
                     /gene="dapB"
                     /locus_tag="ACHKNB_RS21765"
                     /old_locus_tag="pgaptmp_004353"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGU4197747.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1969 a         2162 c         2467 g         2065 t
ORIGIN      
        1 tataggaatt taattcaaaa atagagtggt cttaagttta atacctcaat gtgtatcaca
       61 atatccacat tctttgtagg ggagtctgga gattgagtag atattcttgt tcagaatgta
      121 tcagccgatg gttctacgat tcttaagcca cgaagagttc agaaagtgta acggcatgtc
      181 tgcaaaagcc agcatctttt gactatctgg caaccggtag tgtgttctct cacgcatcat
      241 aaaagcagca ggcataaaaa aacccgcttg cgcgggcttt ttcacaaagc ttcagcaaat
      301 tggcgattaa gccagtttgt tgatctgtgc agtcaggtta gccttatgac gtgcagcttt
      361 gtttttgtgg atcagacctt tagcagcctg acggtccacg atcggttgca tttcgttaaa
      421 tgctttctgt gcagcagctt tgtcgccagc ttcgatagct gcgtatactt tcttgatgaa
      481 agtacgcatc atagagcgac ggcttgcgtt gtgcttacga gccttttcag actgaatggc
      541 gcgcttctta gctgatttga tattagccaa ggtccaactc ccaaatgtgt tctatatgga
      601 caattcaaag gccgaggaat atgccctttt agccttcttt tgtcaatgga tttgtgcaaa
      661 taagcgccgt taatgtgccg gcactcgtta cgtagtgatg gcgcaggatt ctaccagctt
      721 gcggggtgtg aatacagctt ttccgcgata aaaattgcag caggcggtca ctttcttccc
      781 gtgatttgcg ccatggcaat gaaaagccac ttctttctga tttcggtact caatcgccgg
      841 ttaaccttga ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat
      901 gaagctgata cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac
      961 tattggtaat ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagga
     1021 agaagggcgc aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga
     1081 actgtttgct accgataaag ccccggcacg gctgacccgg ctgcgggaaa aactgcgtta
     1141 ccttgcagag tgtggcgttg attacgtgct gtgcgtgcgt ttcgacaggc gtttcgcggc
     1201 gttaaccgcg caaaatttca tcagcgatct tctggtgaag catttgcgcg taaaatttct
     1261 tgccgtaggt gatgatttcc gctttggcgc tggtcgtgaa ggcgatttct tgttattaca
     1321 gaaagctggc atggaatacg gcttcgatat caccagtacg caaacttttt gcgaaggtgg
     1381 cgtgcgtatc agcagcaccg ctgtgcgtca ggcgctggcg gatgacaatc tggctctggc
     1441 agaaagttta ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt
     1501 agggcgcact ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt
     1561 gaaaggggtt tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc
     1621 aaacattgga acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt
     1681 agatgttgca atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg
     1741 caatgagcag cgatttgcgt cgctggacga actgaaagcg cagattgcgc gtgatgaatt
     1801 aaccgcccgc gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc
     1861 gaaacacgga accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac
     1921 agggttcccg atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac
     1981 tgatgatgat ctgtacggca tcatccgtgc ggctaaaaaa ggcaaaaaaa ccttcattct
     2041 gcatgatggc cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat
     2101 tctgaaagac attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc
     2161 tggctgggac tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc
     2221 gggtgagaaa ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca
     2281 ggttgacggt caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc
     2341 gtacctgacc atggacttca aaactgaagc caacatcatc cgcgcgctgg gcaaaatcat
     2401 cggcaacggt cacctgcaca aaggcgcgaa gccagttcac tggtgcgttg actgccgttc
     2461 tgcgctggcg gaagcggaag ttgagtatta cgacaaaact tctccgtcca tcgacgttgc
     2521 ttttcaggcg gtcgatcagg atgcactgaa agcaaaattt gccgtaagca acgttaacgg
     2581 cccaatctcg ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat
     2641 ctctattgca cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct
     2701 ggcgaaagat ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct
     2761 cggcacggta aaaggtgcgg agcttgagtt gctgcgcttt acccatccgt ttatgggctt
     2821 cgacgttccg gcaatcctcg gcgatcacgt taccctggat gccggtaccg gtgccgttca
     2881 caccgcgcct ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac
     2941 cgctaacccg gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg
     3001 cgtgaacgtc ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct
     3061 gctgcacgtt gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat
     3121 catcttccgc gcgacgccgc agtggttcgt cagcatggat cagaaaggtc tgcgtgcgca
     3181 gtcactgaaa gagatcaaag gcgtgcagtg gatcccggac tggggccagg cgcgtatcga
     3241 gtcgatggtt gctaaccgtc ctgactggtg tatctcccgt cagcgcacct ggggcgtacc
     3301 gatgtcactg ttcgtgcaca aagacacgga agagctgcat ccgcgtactc tcgaactaat
     3361 ggaagaagtg gcaaaacgcg ttgaagttga cggcatccag gcgtggtggg atcttgatgc
     3421 gaaagagatc ctcggcgatg aagctgatca gtacgtgaaa gtgccggaca cattggatgt
     3481 atggtttgac tccggatcta cccactcttc tgttgttgac gtgcgtccgg aatttgccgg
     3541 tcacgcagcg gacatgtatc tggaaggttc tgaccagcac cgtggttggt tcatgtcttc
     3601 cctaatgatc tccaccgcga tgaaaggcaa agcgccgtat cgtcaggtac tgacccacgg
     3661 ctttaccgtg gatggtcagg gtcgcaagat gtctaaatcc atcggcaata ccgtttcgcc
     3721 gcaggatgtg atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga
     3781 ctacaccggt gaaatggccg tttctgacga gatcctgaaa cgtgctgccg atagctatcg
     3841 tcgtatccgt aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccagcaaa
     3901 agatatggtg aaaccggaag agatggtggt actggatcgc tgggccgtag gttgtgcgaa
     3961 agcggcacag gaagacatcc tcaaggcgta cgaagcatac gatttccacg aagtggtaca
     4021 gcgtctgatg cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga
     4081 ccgtcagtac accgccaaag cggacagtgt ggcgcgtcgt agctgccaga ctgcgctgta
     4141 tcacatcgca gaagcgctgg tgcgctggat ggcaccaatc ctctccttca ccgctgatga
     4201 agtgtggggc tacctgccgg gcgaacgtga aaaatacgtc ttcaccggtg agtggtacga
     4261 aggcctgttt ggcctggcag acagtgaagc gatgaacgat gcgttctggg acgagctgtt
     4321 gaaagtgcgt ggcgaagtga acaaagtcat tgagcaagcg cgtgccgaca agaaagtggg
     4381 tggctcgctg gaagcggcag taaccttgta tgcagaaccg gaactgtcgg cgaaactgac
     4441 cgcgctgggc gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta
     4501 taacgacgca cctgctgatg ctcagcagag cgaagtactc aaagggctga aagtcgcgtt
     4561 gagtaaagcc gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa
     4621 ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga
     4681 aaaacgtaag tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg
     4741 ctggtggtag tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt
     4801 gctctggggg atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat
     4861 ggcgcggcgt ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt
     4921 attgcgattg gtattagcgt gatcctggca gtgatgatgt atcgctcgaa ggccacgcag
     4981 aagctaaaca atatcgctta tgcgctgatt attggcggcg cgctgggcaa cctgttcgac
     5041 cgcctgtggc acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc
     5101 gccaccttca accttgccga tactgccatt tgtgtcggtg cggcactgat tgtgctggaa
     5161 ggttttttgc cttctaaagc gaaaaaacaa taataaaccc tgccggataa ggcctacaga
     5221 ttgctgcgaa atcgtaggcc gcatccggca aaaatcctca aaatataaga gcaaacctgc
     5281 atgtctgaat ctgtacagag caatagcgcc gtcctggtgc acttcacgct gaaactcgat
     5341 gatggcacca ccgctgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggc
     5401 gatgcttctc tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa
     5461 accaccttct cgctggagcc cgatgcggcg tttggcgtgc cgtcaccgga cctgattcag
     5521 tacttctccc gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt
     5581 tttaccgcaa tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc
     5641 attaccgttg atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg
     5701 ctggaaatcg atccggcact ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg
     5761 tttttgtgcc ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg
     5821 cgcaccgata tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg
     5881 tgagcgtggg gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat
     5941 tttctccgca cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagtc gcgatttgac
     6001 ggtatttgac gcgacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag
     6061 tcgccgtggc gaagaatcta ttctcatcgg tcacgccggg cacccggaag tggaagggac
     6121 aatgggccag tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt
     6181 gtggaaactg acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc
     6241 ggtggatgac acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg
     6301 tccgcgcaaa gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct
     6361 ggcagaacag gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg
     6421 tctggcggag ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga
     6481 tatccaggaa gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc
     6541 tccggatatt ctggtgcaga atgtggtggc acgtttgcag cagctgggcg gtggtgaagc
     6601 cattccgctg gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt
     6661 cgatattcgt gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt
     6721 gttaccggca ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc
     6781 ggcattgacg atgccgtcgc cattgccgcc gcgatttttg cacccgaact cgacctgcaa
     6841 ctgatgacca ccgtcgcggg taatgtctcg gttgagaaaa ctacccgcaa tgccctgcaa
     6901 ctgctgcatt tctggaatgc ggagattccg ctcgcccaag gggccgctgt gccgctggta
     6961 cgcgcaccgc gtgatgcggc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt
     7021 gttgagcaca accgaaagcc gctcgggata ccggcgcttc tggcgattcg ggatgccctg
     7081 atgcgtgcac cagagcctgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg
     7141 ttactttcac aatgcccgga atgcaagccg tatattcgcc gtctggtgat catgggtggt
     7201 tctgccggac gcggcaactg tacgccaaac gccgagttta atattgctgc cgatccagaa
     7261 gctgctgcct gtgtcttccg cagtggtatt gaaatcgtca tgtgcggttt ggatgtcacc
     7321 aatcaggcaa tattaactcc tgactatctc tctacactgc cgcagttaaa ccgtaccggg
     7381 aaaatgcttc acgccctgtt tagccactac cgtagcggca gtatgcaaag cggcttgcga
     7441 atgcacgatc tctgcgccat cgcctggctg gtgcgcccgg acctgttcac tctcaaaccc
     7501 tgttttgtgg cagtggaaac tcagggcgaa tttacctcag gcacgacggt ggttgatatc
     7561 gacggttgcc tgggcaagcc agccaatgta caggtggcat tggatctgga tgtgaaaggc
     7621 ttccagcagt gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttattgg
     7681 catgcagtca ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt
     7741 tctaattatc agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt
     7801 ctatgcaaat taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc
     7861 gggagccggg gggcgtatgg gccgccagtt gattcaggcg gcgcttgcat tagaaggcgt
     7921 gcagctgggc gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga
     7981 gctggctggt gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtgaaaga
     8041 tgattttgat gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt
     8101 ttgtcgccag catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa
     8161 acaggcgatt cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt
     8221 tggcgttaac gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac
     8281 cgatatcgaa attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc
     8341 actggcaatg ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt
     8401 ctacagtcgt gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt
     8461 gcgtgcaggt gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct
     8521 ggagatcacc cataaggcgt ccagccgcat gacatttgct aacggcgcgg taagatcggc
     8581 tttgtggttg agtggtaagg aaagtggtct ttttgatatg cgagatgtgc ttgatctcaa
     8641 tagtttgtaa ccacaaaata ttt
//
LOCUS       DAZFVO020000186         8656 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFVO020000186 DAZFVO020000000
VERSION     DAZFVO020000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.187"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            371..1861
                     /locus_tag="ACHKNB_RS21770"
                     /old_locus_tag="pgaptmp_004354"
     CDS             371..1861
                     /locus_tag="ACHKNB_RS21770"
                     /old_locus_tag="pgaptmp_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HGU4197748.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTTWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEALQKNRGEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLPELNACYSQKLIRWHEQSFGL"
     gene            complement(1983..2555)
                     /locus_tag="ACHKNB_RS21775"
                     /old_locus_tag="pgaptmp_004355"
     CDS             complement(1983..2555)
                     /locus_tag="ACHKNB_RS21775"
                     /old_locus_tag="pgaptmp_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197749.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2641..2847)
                     /locus_tag="ACHKNB_RS21780"
                     /old_locus_tag="pgaptmp_004356"
     CDS             complement(2641..2847)
                     /locus_tag="ACHKNB_RS21780"
                     /old_locus_tag="pgaptmp_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4197750.1"
                     /translation="MNTPVSLMADQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            3704..3928
                     /gene="hha"
                     /locus_tag="ACHKNB_RS21785"
                     /old_locus_tag="pgaptmp_004357"
     CDS             3704..3928
                     /gene="hha"
                     /locus_tag="ACHKNB_RS21785"
                     /old_locus_tag="pgaptmp_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HGU4197751.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(4260..4457)
                     /locus_tag="ACHKNB_RS21790"
                     /old_locus_tag="pgaptmp_004358"
     CDS             complement(4260..4457)
                     /locus_tag="ACHKNB_RS21790"
                     /old_locus_tag="pgaptmp_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197752.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
     gene            4656..5225
                     /locus_tag="ACHKNB_RS21795"
                     /old_locus_tag="pgaptmp_004359"
     CDS             4656..5225
                     /locus_tag="ACHKNB_RS21795"
                     /old_locus_tag="pgaptmp_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HGU4197753.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5486..5869
                     /locus_tag="ACHKNB_RS21800"
                     /old_locus_tag="pgaptmp_004360"
     CDS             5486..5869
                     /locus_tag="ACHKNB_RS21800"
                     /old_locus_tag="pgaptmp_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4197754.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            5866..6279
                     /locus_tag="ACHKNB_RS21805"
                     /old_locus_tag="pgaptmp_004361"
     CDS             5866..6279
                     /locus_tag="ACHKNB_RS21805"
                     /old_locus_tag="pgaptmp_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HGU4197755.1"
                     /translation="MNGLKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCERLF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRNPE"
     gene            6285..6464
                     /locus_tag="ACHKNB_RS21810"
                     /old_locus_tag="pgaptmp_004362"
     CDS             6285..6464
                     /locus_tag="ACHKNB_RS21810"
                     /old_locus_tag="pgaptmp_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197756.1"
                     /translation="MQHNCLITPDTPLPDPDVVRRFVLRTVVQWLCLLMLLFMRHSDR
                     GDIDKHHLRANSYTE"
     gene            6585..7208
                     /locus_tag="ACHKNB_RS21815"
                     /old_locus_tag="pgaptmp_004363"
     CDS             6585..7208
                     /locus_tag="ACHKNB_RS21815"
                     /old_locus_tag="pgaptmp_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGU4197757.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            7297..7530
                     /locus_tag="ACHKNB_RS21820"
                     /old_locus_tag="pgaptmp_004364"
     CDS             7297..7530
                     /locus_tag="ACHKNB_RS21820"
                     /old_locus_tag="pgaptmp_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197758.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(7550..7759)
                     /locus_tag="ACHKNB_RS21825"
                     /old_locus_tag="pgaptmp_004365"
     CDS             complement(7550..7759)
                     /locus_tag="ACHKNB_RS21825"
                     /old_locus_tag="pgaptmp_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4197759.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            8258..>8656
                     /gene="tssB"
                     /locus_tag="ACHKNB_RS21830"
                     /old_locus_tag="pgaptmp_004366"
     CDS             8258..>8656
                     /gene="tssB"
                     /locus_tag="ACHKNB_RS21830"
                     /old_locus_tag="pgaptmp_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HGU4197760.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PELVARQIPRLKAMLAMRSLLRDLKANLLDN"
BASE COUNT         2170 a         2004 c         2289 g         2193 t
ORIGIN      
        1 gtactcagca ttcagcgcag gaactgtcat cttctttatt ttattcttct tcaggagggc
       61 tgttttctga aagcgttact ccggggaaac tgtttatgtt tattcccggc aggaaacccg
      121 tacctgcaat accctggatg atgctttttg ctggtacgtt gttgtgtgaa aattaatcaa
      181 catgtgtgct ccggagagcg cccgtagacg cacagattca ttctgacatt ctttagcgac
      241 aaaatatcga gtggttatta tttaacctga aaatcgacag aaatttttat cctgtattga
      301 ttctgctgct atttttgcta ttagtagcaa tagttgcggt actttatgtt ctgtgtgttg
      361 tgaggtcatt atgccatact ttcagtatcc tgatgagttt cccctgagct cgctgcctcc
      421 actcattcgt gatgccgtta ttgaagctca gcaaatcacg caggctcccc tggggcttgt
      481 ggcagcatct gccctgggcg ctgtttcact tgtctgccag aatctgattg atgtctgcag
      541 actgaataca ctacgggggc cggtttctct tttcttactg acgcttgctg agtccggaga
      601 acgaaaaacc gcagtggaca aattattaat ggagcccctg taccagcagg agatgctgtt
      661 atattccaga cacaaaaatg aactgacgac atggaaaaat aaggaagagc ttttaaaggc
      721 gcagaaaaaa gcattgttgt caaaactgaa taaagaactc cgcaaaggcg cggatgagtc
      781 agaaacgctc agacaactgg aagcgcttca gaaaaacaga ggggaaaaac ctgtaagata
      841 taaattcatt tttaatgatg ccacgacagc agcaattaaa gatcagcttt gtggtcagtg
      901 gcgttcagtg ggtatcatgt ctgatgaagc cggtataata tttgatgggt ataccctgtc
      961 tgagctgcct tttattaata aaatgtggga tggctccgta ttgtcggtgg acagaaagaa
     1021 tgaacccgaa cagatgattg aaaatgccag aatgacatta tccctcatgg tacaacccgg
     1081 tctttttgac cgatatatgg agcgaaaagg ttctgttgcc agggacagtg gttttcttgc
     1141 ccgttgtcta atcagtaaac ctgccaccac gcaggggaaa cgctttatta acggagctgt
     1201 aaccccggga ggatcattaa cggcatttca tgaacgatta atggaactgg ccagaggcag
     1261 tattgaaaaa tccagtaagg atgaacgtta ttgtctgcat ttttctcctg aagcgcaaaa
     1321 aatatttatt gatcactata atgttctgga gcaggatttg tctccttccg gacccttaag
     1381 ccagtttcgg gggcatgttt caaaaaaaac agaaaacatt gcgagaattg ctgcgttatt
     1441 gcagtatttc agtcagggtg aagggaaaat ctctgccgat atcatgacgt ctgctgtcgt
     1501 aataagttcg tggtacacag atgaatataa aaaattattt gcgttacctg atgaatctga
     1561 actgcagctg caggacgccc gggagctgct cgactggctc attgaggagt gccgcggaga
     1621 atgcccgccc cgtgtcagga aaaattatat tctccagtgt ggcccgggcc ggttcaggaa
     1681 caggaaaaag ctgaacgtcc tgctgaacat tcttgcatct caactcaggc tctctgtcgt
     1741 gcaggaagga aaaaccacct atgtcctgct ttccgaaata aacaatatca cgctgcctga
     1801 actgaatgcg tgctacagcc agaaactgat aagatggcat gagcaatcct ttggcttata
     1861 atgttccctg ttgctgagac ggattattca gtggtggtat cgtagtatgc ccgtcacccc
     1921 ccgcttctgc atgagggtga cggaggaaaa gtaagtgtgc ggaggcccgg gagcgttatg
     1981 aatcaacagg cgtataacag gtactcgtca atcatcagtt ccagctcgcg cttttcccgt
     2041 ttgcgcgcca cagcccagtt ttcaagctct gcatgtgatt tatgctttaa ctcttcagtc
     2101 cagggctgca tggagtgggc acacatcaga tcataccagc acagccatat cagcgtgtgg
     2161 tagagatcac gatcgtcgaa acgccggagc aggctgttca gcctgtttgc cagccggttt
     2221 tcacccagac gatgtagctc actgagtaaa acgccgccca gattaaaatg tgtaagcagc
     2281 atgatttcat gggtctgaat gctggtcatt aacccttcct gaatggcagg aatgaacggt
     2341 ggtgttcccg ggttgacgac ctgatagagt ccctgtggtg ccactgagat gatagtgcct
     2401 ttttctggca gaaaaaagtg tgccagggtc aggcggtgat aaccatgaag atgcggagac
     2461 cccattccat gagcgacgat aagcctcgtc acgcttgtca tccatttctg atgctggatg
     2521 agcagcagct cacaccggtt ttcaagtttt tgcataatat tctctccttt agcgcgcagc
     2581 aaaatgcctc cgggggccgg atcatgcctt tgctgcggtt gaacggataa cggtcaggaa
     2641 ttacggacgg gactgtgcaa tacgcgcctg cagccaggtt tccacttcac ttttcagcca
     2701 gcgggaactg cggcccagtt tgatgggggc aggaaaggca ccatccttga tgagcttgta
     2761 aaaccattta tcggtcaggc cggtcagttg agtgataaat gccatgtcga ccatctggtc
     2821 agccatcagt gaaactgggg tattcatgaa aatgttcctc cggatgttgt taatctcaac
     2881 cggcgttggt gatgaaaaca acgcaccacc ttcaccggca taacaaccgg ggagaaacgt
     2941 aaaaaataat cgggaatgtg accgtcagga gataaacggg gggattgcgg tggctgagcc
     3001 gtaccggtat gtcgggtgat tgttgcgaaa cgatgcttcc gcacgcgcac gggaggactt
     3061 tcgtgagaca aggggaaaac tggcgttctg ataattgcag agttgttttt gaaggcggtt
     3121 gtggtgcatc agcctgtgac ctgtgggcag gcgacaggta tcactggtgg gataagactg
     3181 tctgattggt attataaata tataagagag ctcaaacaca tgagttcata tatgtgaaat
     3241 aaatctgtga gtgtgatcag cagaccagtc tacccagtag caacagattg cgttgtactc
     3301 acagggttat ccctgataac tggcgtataa cctgcgtaca taaaacgtac ctgcaatgca
     3361 cctgaacaga gtgcattaag catccgcctg agcggtaaac gtatccgatc tggtgagtga
     3421 cagtctgaga gcattctcag taatcgcccc cttgtatcct ggaaattggt taagagcgat
     3481 tagatttggc aaggtgtatg catccagcag cttaacacct cagctaactg gctggttacc
     3541 ttcaggttgt cgcaatggca tcggacatcc ggtgtcggat aactaaacgt agcgttaagg
     3601 cgatattgct gagagaccgg ttaagtggtg gcacgggagt aatgagtttg gggagggtac
     3661 cagaatgcca gtcaggtgcg gtatgctggc agtatcacgt tgattgcagc ataaagtaac
     3721 gagagacgaa tggaagttaa aaccagagca gtttcgtcgt tgttcatccc gggagacgct
     3781 ggaaaaagtg attacccgca cacgttataa acttacccct gcggagctgg aagcctttaa
     3841 ctctgcggtc gatcaccggc tggcagaact gacaatgaac aaactttacg atcgcgtgcc
     3901 ggcttccgtc tggaaatatg tcacctgaat cttgaatccg cgctacgatg actctggtgt
     3961 tatctgtgca ccggaagatc gggaacatta agcagtataa cggcaacaaa gaattcgaaa
     4021 tcaaaaggga atggtgtaca gaggggcagg cgttcaggca ttaccggcct gtatcttctg
     4081 aaggggactc gtatttgtcg gggctgagta ttccgctcga ttcgatcacg gattggcaga
     4141 aacctgactg tgattgattc aggtaccatt ttgcagggat ccggtgcgtt actgtctggc
     4201 acaacaaacg cagcgggtcg ctctgccccg ccgattcctg ataattcctc ccctgtaacc
     4261 tactgcaaca gtacaaatac actgacgcct gaaacggtaa ccagaatcag caccagcagt
     4321 aacgtcagta aacagagcag acgatcggtc ttccggtaat aaccgaaggg gtcgacagcg
     4381 ccacaatacg ggcaacacac tgtattatga gtaattgcgt ttccacagtc cctgcacttc
     4441 tttttgcgta tcacgatcgc tccctctcca cgccttatcc gtaacctgtt ttttacatta
     4501 aaaaaacaac gccgggaatt caatcgtcag tccggagacg gccgttcggg ttatcacaga
     4561 gtgcctgaga cagtgtcctg ccggaggtca cctcaggggc tctgcgcatt ttttacgacg
     4621 tggctattcc gtatgaacca tacggagatt taaccatgac ctacaaatac aaccccttct
     4681 ggcagcaacg tattcgtgag acggtgcggc acgcactgga tgttcatccc cgcctgacgg
     4741 cacttcgggt tgatctgcgt ctcccggatg tgccggcagc aacggacgcg gctgtgatat
     4801 cccgcttcat caatgccctg aaagcccgaa tcgacgctta tcagaaacgt aagcatcggg
     4861 aaggtaaacg cgtgcattcc tcgaccctgc attacgcctg ggcccgggag ttcggggagc
     4921 ttaaaggcaa aaaacactat cacctgctgc tgctggtcaa ccgggatacc tggtgtcgtg
     4981 ccggcgatta ccgcgctccg gggtcactgg ccgggatgat taaacaggcc tggtgcagtg
     5041 ccctgggagt ggatgccgga cgttatgaca cgctggcaca ttttcctgac aagccagcgg
     5101 tgtggctgga acgcgatgat gaaaccggct ttcagcaggt gctggaacgt tctggctatc
     5161 tggcgaagga gcataccaaa gctcgcggca ccggtgagcg caattttggc tgtagccgga
     5221 gttgaacacg acaggcgcac caatacccgg caaaccaaaa gtatgggttg ttgtctggcg
     5281 gcaacgatgt atccactcgc ccactgactg ttttttacgt cagtgggcca ttgtgtgaca
     5341 accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca gattttaact
     5401 gaaccgccat tcacaccctg accacgctgc ccgtaccggg acggctgttg cctgcgtgtc
     5461 gtctggcggc tgaggaacat atactatgta cgcaaaatcc tttctcgctc ttgatggcaa
     5521 cggacgtctg acgggggccc gtaccgcaca gactgcgcct tacgaccgct acacctgcca
     5581 cctttgcggc agtgcactca gataccaccc gcaatacgac actgaacgtc cctggtttga
     5641 acacactgac gacgggctga cggagcacgg tcatgagtgc ccttatgtca gaccggagcg
     5701 cagggagata caactgatta aacgtctgca gcaattcgta ccggatgcct tacccgtagt
     5761 acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg agcggtactg
     5821 cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa cggtctaaaa
     5881 acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactctgc gctcagcaga
     5941 aatatccggc agtgtttctg cctttcctgt catggccggt tgattctgca gacagatgct
     6001 cagggggcgt ggtttgagca cgatttacat gccctgagcg agcaacagaa ggcggactgt
     6061 gtggtgctga atccggagaa gtcccaccct tacattgagg atatggcgat gtttttatcg
     6121 ccattaccgg tcgtgctgga gtggcattgt gtgatgtgtg agaggctttt tcatgggaag
     6181 aagttctgtg aggcctgtgg tacggggatt tattgcagag cggtatgtac aaagtctgtt
     6241 tacaggtacc agccagattt gctccggaac ccggagtgag gaggatgcaa cataattgtt
     6301 taatcacgcc agacacgccc ctgccggatc cggatgtggt ccggcgtttt gttctgcgga
     6361 cggtggttca atggctgtgt ctgctgatgc tcctctttat gcgacattca gacaggggag
     6421 acatcgataa acatcatctg cgggcaaata gttacacaga atgatcattg ctaccggtta
     6481 tggcaaaaat tggtgatgac caggtgaacc atcggtgctt tcgttgattc ttatggactg
     6541 ataaagtttt gtcagctgaa tctttatacg gaattctttt cagtatgcgt acacgaattg
     6601 actatcttgc ggataaatac agttttactg aactgaatga acttccacgc cttcaccagc
     6661 agtggcagga tgttctggag gagtgccggc agacagaggc cgggccggaa gaacggctgc
     6721 gtattgcact gctgaatgtg gattacgtca ccagttttga actgcctttt cgcttgttgc
     6781 ttacccgtac accacaactg attgccgcgc tgcgtgaaga atggggcatc agccagaaaa
     6841 atgtggtgtt caatgataaa cgatttggct gcgtgtacag cctgaaggcc agcctttccg
     6901 gtgtgccgga tacataccgg tatcatctgt ctcatcgtat tcgccgggtg attgggaatg
     6961 aaaacacatc atcgccatac cagcagattg cccgggaagt gaaagcgccc cgtgaacggc
     7021 tgaagcatgc gctggaggcg gggttgctgg tgactgcact ggacggcctg ttctggttcg
     7081 gcagtcagcg tattgcggct gatgtcctga ggttgagaaa gagcggaatg ccggtggtga
     7141 caacgaccgc ggaggtgcat gataatctga cgggaacaac ccgcaaagta ccggcctact
     7201 atctctgatt cgaccatcag ggggaatatt gctctggcag cagcagtacg acattcttta
     7261 tccccgagcc tgaaaacatg tgctaaggaa acgggaatgg caaggtccga ttacgatatt
     7321 atcaatctgt ctccggaaca tgaactgaat gagtggctgg cagagagagg ttatgccgga
     7381 caggcggata accgtaaccg ggtggcagaa attgttaccc gcaaattgca ggacagcttt
     7441 tatatcaacg tttcctggga tgcgctgaac acggcataca gtgaacaccc ggagtggttt
     7501 tctgggcttg tctccgggga tgagaattaa taagtggatt atgctgccat caggcagtgt
     7561 gattgccggc agggggttct ttccggggtt cgggtttgaa ttttcccagg aagttgtaca
     7621 tcggccgcat gcagggatgc ggatagccgt cccgcataaa caccacccgg gtttcctcaa
     7681 cactggtcac ggtgacaagc gcgccatgat cgtctctgta tcgctgattg atttttggta
     7741 atggaatatc ttctgacata gacttcagaa cggtaaccgt tcactaaatt ttcatgtggt
     7801 atcactgaat caggagacga cgtttcctga tgtgcgttgt taattttctg tttttaaaaa
     7861 atattttctc tctcttcttt attttccgtg tcttttattg ctctcctctt tcatgtcctt
     7921 ctctttttct gatatgccct taaatgctgc cgaatcatca ctcctcgtga atgtcagtgc
     7981 tggtaatcat tcggtataac catctgtatc aaaagcacta accggaataa ttttctgcat
     8041 ttttgtatta cccctggata taagttttga taaatgctga ttttgcgata tgtatcagaa
     8101 taaagatctg gttattggca ttaaaacgat cgatatgtct gtcatattga gaatttgcag
     8161 atcttcgtcc tataatgggt gaaattaaat cagtgcacaa ggggaggcct tctgtggcga
     8221 tgggctccct gggatgcagg tttcacagga gaacgtcatg agcagttcgt ttcagaatga
     8281 gatcccgaag gcaagggtga atctgaaatt agacctacat acaggtggtg ggcagaagaa
     8341 agtggagctt ccgctcaaac tgctggttgc cggtgatttc agtaacggac aggaagagcg
     8401 cccgctgtca gagcgcataa aaatggatgt taataaaaat aactttaatg ctgttctggc
     8461 tgatatctcg ccgtcagtac gcctgaatgt ggagaacaca cttgccggtg atggtactga
     8521 agagagcatc agcctgactt tccgtaatat gaacgatttc acgccggagc tggttgcccg
     8581 acaaattccc cgtcttaaag ccatgctggc catgcgaagt ttgctgcggg atctgaaggc
     8641 gaatctgctg gataac
//
LOCUS       DAZFVO020000187         8652 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFVO020000187 DAZFVO020000000
VERSION     DAZFVO020000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.188"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            54..1487
                     /gene="cscA"
                     /locus_tag="ACHKNB_RS21835"
                     /old_locus_tag="pgaptmp_004367"
     CDS             54..1487
                     /gene="cscA"
                     /locus_tag="ACHKNB_RS21835"
                     /old_locus_tag="pgaptmp_004367"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGU4197761.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1495..2490)
                     /locus_tag="ACHKNB_RS21840"
                     /old_locus_tag="pgaptmp_004368"
     CDS             complement(1495..2490)
                     /locus_tag="ACHKNB_RS21840"
                     /old_locus_tag="pgaptmp_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4197762.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2733..2951
                     /locus_tag="ACHKNB_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /pseudo
     CDS             2733..2951
                     /locus_tag="ACHKNB_RS21845"
                     /old_locus_tag="pgaptmp_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2969..4297
                     /gene="dsdA"
                     /locus_tag="ACHKNB_RS21850"
                     /old_locus_tag="pgaptmp_004370"
     CDS             2969..4297
                     /gene="dsdA"
                     /locus_tag="ACHKNB_RS21850"
                     /old_locus_tag="pgaptmp_004370"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HGU4197763.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSLTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4405..5943)
                     /gene="emrY"
                     /locus_tag="ACHKNB_RS21855"
                     /old_locus_tag="pgaptmp_004371"
     CDS             complement(4405..5943)
                     /gene="emrY"
                     /locus_tag="ACHKNB_RS21855"
                     /old_locus_tag="pgaptmp_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HGU4197764.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAHIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5943..7106)
                     /gene="emrK"
                     /locus_tag="ACHKNB_RS21860"
                     /old_locus_tag="pgaptmp_004372"
     CDS             complement(5943..7106)
                     /gene="emrK"
                     /locus_tag="ACHKNB_RS21860"
                     /old_locus_tag="pgaptmp_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HGU4197765.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALIMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7522..8136
                     /gene="evgA"
                     /locus_tag="ACHKNB_RS21865"
                     /old_locus_tag="pgaptmp_004373"
     CDS             7522..8136
                     /gene="evgA"
                     /locus_tag="ACHKNB_RS21865"
                     /old_locus_tag="pgaptmp_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HGU4197766.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8141..>8652
                     /locus_tag="ACHKNB_RS21870"
                     /old_locus_tag="pgaptmp_004374"
     CDS             8141..>8652
                     /locus_tag="ACHKNB_RS21870"
                     /old_locus_tag="pgaptmp_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGU4197767.1"
                     /translation="MKFLPYFFLLCCDLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTPLHDSMRPLT
                     SPKPVNIARVA"
BASE COUNT         2437 a         1998 c         1960 g         2257 t
ORIGIN      
        1 tataaaagtt aacgttaaca attcaccaaa tttgcttaac caggatgatt aaaatgacgc
       61 aatctcgatt gcatgcggcg caaaacgccc tagcaaaact tcatgagcac cggggtaaca
      121 ctttctatcc ccattttcac ctcgcgcctc ctgccgggtg gatgaacgat ccaaacggcc
      181 tgatctggtt taacgatcgt tatcacgcgt tttatcaaca tcatccgatg agcgaacact
      241 gggggccaat gcactgggga catgccacca gcgacgatat gatccactgg cagcatgagc
      301 ctattgcgct agcgccagga gacgataatg acaaagacgg gtgtttttca ggtagtgctg
      361 tcgatgacaa tggtgtcctc tcacttatct acaccggaca cgtctggctc gatggtgcag
      421 gtaatgacga tgcaattcgc gaagtacaat gtctggctac cagtcgggat ggtattcatt
      481 tcgagaaaca gggtgtgatc ctcactccac cagaaggaat catgcacttc cgcgatccta
      541 aagtgtggcg tgaagccgac acatggtgga tggtagtcgg ggcgaaagat ccaggcaaca
      601 cggggcagat cctgctttat cgcggcagtt cattgcgtga atggaccttc gatcgcgtac
      661 tggcccacgc tgatgcgggt gaaagctata tgtgggaatg tccggacttt ttcagccttg
      721 gcgatcagca ttatctgatg ttttccccgc agggaatgaa tgccgaggga tacagttacc
      781 gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc gccaggacga ctttttgcac
      841 aatccgggca ttttactgaa cttgataacg ggcatgactt ttatgcacca caaagctttt
      901 tagcgaagga tggtcggcgt attgttatcg gctggatgga tatgtgggaa tcgccaatgc
      961 cctcaaaacg tgaaggatgg gcaggctgca tgacgctggc gcgcgagcta tcagagagca
     1021 atggcaaact tctacaacgc ccggttcacg aagctgagtc gttacgccag cagcatcaat
     1081 ctgtctctcc ccgcacaatc agcaataaat atgttttgca ggaaaacgcg caagcagttg
     1141 agattcagtt gcagtgggcg ctgaagaaca gtgatgccga acattacgga ttacagctcg
     1201 gcactggaat gcggctgtac attgataacc aatctgagcg acttgttttg tggcggtatt
     1261 acccacacga gaatttagac ggctaccgta gtattcccct cccgcagcgt gacacgctcg
     1321 ccctaaggat atttatcgat acatcatccg tggaagtatt tattaacgac ggggaagcgg
     1381 tgatgagtag tcgaatctat ccgcagccag aagaacggga actgtcgctt tatgcctccc
     1441 acggagtggc tgtgctgcaa catggagcac tctggctact gggttaacat aatatcaggt
     1501 ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact cttccccctt cacgaccttc
     1561 aataatatgc aatgcagctt cccgcccgat aatgtcatgt ggaagctgaa ttgtggtcag
     1621 cggcggtaaa aacagatgcc cgacgccaac cagattatca aagcccatta cggcgacatc
     1681 ctgcgggata cgtaccccct tcgccaggag aacctgataa gcgacaaagg ctgcgcgatc
     1741 gttaccacat atcagaacat caaaatctgg tttgcccggt ttgaagtggg cattgagtaa
     1801 acttgcgaga tcggtgtagt gatcatcacc tgttgccatg tgaaattgtt tcacctcagc
     1861 cagatctcgt ccagcatcac gccaggcctg ctcaaatccc tgccgacgat accctgttgc
     1921 caacgcactt tccggtagcc agaagcataa cggttgacga tagcccgccg cgagcaaatg
     1981 ctgtgttgat tcatattgtg cagtgtaatc atcagggata taactgggta acgctgggtc
     2041 atccgccaca cagttcgcca atacaatatt ttcaccatac agagactcag gcagcgtgat
     2101 atgtcgcagc cccattgtag tatagataat gccatccgga cggtgggcaa gcagctgacg
     2161 tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt aaaaaactat tccagccgaa
     2221 ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca gagaaaggag tggtagccgt
     2281 gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct tgagcgcgca tcttacgggc
     2341 ggaaagatca ggaacataat tcagggtctg gattgcctgc aatacgcggt cacgcgttgc
     2401 aggacgcaca gattctgcat tatgcatcac ccgggagact gtcatcatcg acactcccgc
     2461 caggcgtgcg acatccttta atgaagccat acccaagccg tttgccgtaa aacgggcact
     2521 gtagcagaaa cagacgtcac tggcgagatc caacgatatt ctcaacgccc tatcacccaa
     2581 cacggcaata caataaaaaa taacaataat tgccggataa ttgtactcaa ttccgcctct
     2641 gttctcgcca acgagtaacc atataggcaa gtaagtaaaa catgccaact ggcatcgcca
     2701 acctggagaa gaaaatataa atatggatca ttgtgctgcc gctgtatccc gacatcagcc
     2761 cggaaattat tgcgattgct atcggttcag gtgcaattgg ctgcacgatc gttacggact
     2821 cgcttttctg gctagtgaag caatattgcg gcgctacgct caatgaaaca tttaaatact
     2881 atacgacagc gacatttatc gcttcagtcg tcgctctggc gggcacattc ctgctgtcat
     2941 ttatcatcta agcgcaaaga gacgtactat ggaaaacgct aaaatgaact cgctcatcgc
     3001 ccagtatccg ttggtaaagg atctggttgc tcttaaagaa accacctggt ttaatcctgg
     3061 cacgacctca ttggctgaag gtttacctta tgttggcctg accgaacagg atgttcagga
     3121 cgcccatgcg cgcttatccc gttttgcacc ctatctggca aaagcatttc ctgaaactgc
     3181 tgccactggg gggattattg aatcagaact ggttgccatt ccagctatgc aaaaacggct
     3241 ggaaaaagaa tatcagcaac cgatcagcgg gcaactgtta ctgaaaaaag atagccattt
     3301 gcccatttcc ggctccataa aagcacgcgg cgggatttat gaagtcctgg cacacgcaga
     3361 aaaactggct ctggaagcgg ggttgctgac gcttgatgat gactacagca aactgctttc
     3421 tccggagttt aaacagttct ttagccaata cagcattgct gtgggctcaa ccggaaatct
     3481 ggggttatca atcggcatta tgagcgcccg cattggcttt aaggtgacag ttcatatgtc
     3541 tgctgatgcc cgggcatgga aaaaagcgaa actgcgcagc catggcgtta cggtcgtgga
     3601 atatgagcaa gattatggtg ttgccgtcga ggaaggacgt aaagcagcgc agtctgaccc
     3661 gaactgtttc tttattgatg acgaaaattc cctcacgttg ttccttgggt attccgtcgc
     3721 tggccagcgt cttaaagcgc aatttgccca gcaaggccgt atcgtcgatg ctgataaccc
     3781 tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct ggtggcgtcg cattcgggct
     3841 taaactggcg tttggcgatc atgttcactg cttttttgcc gaaccaacgc actccccttg
     3901 tatgttgtta ggcgtccata caggattaca cgatcagatt tctgttcagg atattggtat
     3961 cgacaacctt accgcagcgg atggccttgc agttggtcgc gcatcaggct ttgtcgggcg
     4021 ggcaatggag cgtctgctgg atggcttcta tacccttagc gatcaaacca tgtatgacat
     4081 gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa ccttcggcac tggcgggtat
     4141 ggccggacct cagcgcgtgt gtgcatcagt aagttaccaa cagatgcacg gtttcagcgc
     4201 agaacaactg cgtaatacca ctcatctggt gtgggcgacg ggaggtggaa tggtgccgga
     4261 agaagagatg aatcaatatc tggcaaaagg ccgttaataa cgtttcaacg cagcatcgca
     4321 atcctttccc tgggtgagcg atgctgccga tggcgcagac ttaagatccc cggtcttacc
     4381 cgctataacc ccctcctttt atcatcaccc aacgcctttc gctgtaaacg gcggtttcgc
     4441 aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc gccatacgga aaatctcatt
     4501 tgcagaaata gaaagtgact gctgggttat ttcattatta atttcattaa gaactcctga
     4561 aagcgaacca tagtatttat ccataatttg tgacgatgaa ttaaacacgg ggttaaattg
     4621 atcgatggtt gctgtcaact gactatggtg taacgattcg cgtcgtcccc acagcgtcat
     4681 tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa aaattactca tactcgaggc
     4741 attggcaaat ttattatctg gcaagcctga aaacgaaatc gttgttaagg gtaaaaagaa
     4801 acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg atgcctgtaa aatcaatcgt
     4861 tggcataaat gtcacagaac gccagtaata gcaaaccgca tacatcaaaa aactgaatgt
     4921 cactaacacc cgcatgtcta ttttattgcc ataacgtcct atcaaaggtg aaatcaatag
     4981 tggcatgatg ccgatgtgcg cataagcaag cccggcccat atcgcattat accccatcgt
     5041 ttcctggagt aactgcggca taaggacgat cgctccagag taaaataaat acgcgcaggt
     5101 gatactcaca ataccaatgg tgaaattacg agacttaaac aaactgagat caagaatcgg
     5161 attctctgag gtcgactccc aaatgactaa agagatcaga gaaataactg atactactgt
     5221 taatattatt attgtactcg agttgaacca atccagatcg cgccctttat caagcataat
     5281 ttgcaagcca ccaacaccga gcactaacag ggtcagtcct ggtagattca tttttaccgg
     5341 tgaagtttca gtttctcttc ctttaagtaa ggttaagcat aatgtcagga cgataatccc
     5401 catagggaca ttgattaaaa atatccaacc ccagctaaag ttatcgcaaa tataaccgcc
     5461 caatatcggc ccacatatcg gagcgataat cacggtcatt gaccataatg ccagagcaaa
     5521 tgttcttttt tctggtggat aattcctcaa taataaactc tgtgacagtg gaattaacgg
     5581 ccccgccatt aatccctgaa cgactctaaa aaatatcagc acatcaagat tggtcgataa
     5641 actacacatt aaggaagaca gcgaaaaaaa agtgactgaa agtaaaaata accttaattc
     5701 gcctattctt tgtgccagcc tgccggtaac agggatcgca atggcatttg ctacgccaaa
     5761 cgaggtgata acccaggtgc cttcgtctgt tgatgctccc agaaagccag atattgtcgg
     5821 tattgcgacg ttagaaatag tggaatccaa catttgcata aatgtcgcta atgacaatgc
     5881 aatagtgacg caccataacg tcccaccggt taatggtgcc ggagttgatt tagtgattgc
     5941 cattaaagtt gtccattatg cgaaataatg ttgctaattt ctttttctat cggactggta
     6001 tcgataacta aagccttact ggtataagcc ggcatggagg tcacggttga agccagctca
     6061 ggcatctcgg caatgtcttc gttcttcgta tcaatagttg ctgtcatcga taaaccaata
     6121 cgcaagggat gttccatgag ttcttttgga tcaagagaaa cttcaaccgg tacacgctga
     6181 acgattttga tccagttccc tgtcgcattt tgtgcaggta ataaggagaa cgcattgccg
     6241 gttcccatat tgatccctgt cacccgacca tgaaacacaa cattttcacc ataaagatcg
     6301 ctgataatat tgaccgattg accaatccgt acatccgtaa gttgtgtttc tttaaagttg
     6361 gcattaaccc acatttgacg tgccggtacg acagccatta acgattgtcc ggggctcact
     6421 gtttcgccga cctgaacact tctctgggca atatagccgg taaccggact cttaatatcc
     6481 gtacgtttaa gcgccaacca ggcttcttta gttgcatccg ccgcttcaac gacttgtggc
     6541 tgacggttta atggtgtgtt cattattaaa gctttattcg ctttataagc ctggatagcg
     6601 gcattcaatg ccgctttgct acttattaac gtatctttgg tatgctccag cgtttctttt
     6661 gaaataaccc cctgcttcgc taacggcact cgacggttat aatcttctaa agattgttga
     6721 tactgaatac gtgctgaagc gacttcggca ctgtattgtt tatcctgtaa gtatagttta
     6781 ttcgtttgcc gaacaatatt tgccagatta tttttagctt tattgagtgc gatagtggca
     6841 tcagttttat ccagtgaaac taaaatgtca ccttgtcgaa cgtagttcgt atctttatga
     6901 ttaacgacag tgacactacc tgagacttgt gcagaaattg gatctgcatt ccccgtgaca
     6961 taggcgtcat ctgtactaat catgtcttct aattccattg accaataggc ataggcacct
     7021 gaaaacgcaa taaataaaac taccgccaat aaagaaaagt attttcttct gttagaatgt
     7081 tttttatttg aattaatctg ttccactatt atctctcatt tctcatagat gaaatttatg
     7141 aattgaatga aaagagcatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat
     7201 cccaggaacg ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac
     7261 cgtggctttt gcaatacaat tcttacgcct gtaggattag taagaagact tatagtgcca
     7321 acttgaaact ataaatcatc ggtacaatcc ctgattttat tgttgacatt tcatttatgc
     7381 cgactattta tatggtatac ttgtcgaatt atcttaaagg aagctcagat tttcttattt
     7441 ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa gggagatgct
     7501 tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc
     7561 gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttgactgaa
     7621 gggggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc
     7681 gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc
     7741 ggaattatta ttattgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat
     7801 gctggcgcta atggattcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt
     7861 gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt
     7921 ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg
     7981 tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa
     8041 actgtcagca cttataaaag tcgcctgatg gaaaaattag aatgtaaatc actgatggat
     8101 ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt taccctattt
     8161 ttttcttctc tgttgtgatc tttggtcgac cataagtttc gcagacgaag attacatcga
     8221 atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc tgagcaacaa
     8281 ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac
     8341 ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg ctgattattt
     8401 aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca
     8461 aaaagcaatg gacgcgctgg aagaaggtga agtcgatata gtgttatcac atttagttac
     8521 ttcgccgcct cttaataatg acattgctgc aaccaaacca ttgataatta cctttccggc
     8581 gctggtaacc ccccttcacg actcaatgcg accgcttacc tcaccaaaac cagtaaatat
     8641 tgctcgggta gc
//
LOCUS       DAZFVO020000188         8626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFVO020000188 DAZFVO020000000
VERSION     DAZFVO020000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.189"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(143..219)
                     /locus_tag="ACHKNB_RS21875"
                     /old_locus_tag="pgaptmp_004375"
     tRNA            complement(143..219)
                     /locus_tag="ACHKNB_RS21875"
                     /old_locus_tag="pgaptmp_004375"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(183..185),aa:Asp,seq:gtc)
     gene            complement(352..1083)
                     /gene="dnaQ"
                     /locus_tag="ACHKNB_RS21880"
                     /old_locus_tag="pgaptmp_004376"
     CDS             complement(352..1083)
                     /gene="dnaQ"
                     /locus_tag="ACHKNB_RS21880"
                     /old_locus_tag="pgaptmp_004376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HGU4197768.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1148..1615
                     /gene="rnhA"
                     /locus_tag="ACHKNB_RS21885"
                     /old_locus_tag="pgaptmp_004377"
     CDS             1148..1615
                     /gene="rnhA"
                     /locus_tag="ACHKNB_RS21885"
                     /old_locus_tag="pgaptmp_004377"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HGU4197769.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1612..2334)
                     /locus_tag="ACHKNB_RS21890"
                     /old_locus_tag="pgaptmp_004378"
     CDS             complement(1612..2334)
                     /locus_tag="ACHKNB_RS21890"
                     /old_locus_tag="pgaptmp_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4197770.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2368..3123
                     /gene="gloB"
                     /locus_tag="ACHKNB_RS21895"
                     /old_locus_tag="pgaptmp_004379"
     CDS             2368..3123
                     /gene="gloB"
                     /locus_tag="ACHKNB_RS21895"
                     /old_locus_tag="pgaptmp_004379"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HGU4197771.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3195..4553
                     /gene="mltD"
                     /locus_tag="ACHKNB_RS21900"
                     /old_locus_tag="pgaptmp_004380"
     CDS             3195..4553
                     /gene="mltD"
                     /locus_tag="ACHKNB_RS21900"
                     /old_locus_tag="pgaptmp_004380"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HGU4197772.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4601..5371)
                     /locus_tag="ACHKNB_RS21905"
                     /old_locus_tag="pgaptmp_004381"
     CDS             complement(4601..5371)
                     /locus_tag="ACHKNB_RS21905"
                     /old_locus_tag="pgaptmp_004381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4197773.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5449..6249)
                     /locus_tag="ACHKNB_RS21910"
                     /old_locus_tag="pgaptmp_004382"
     CDS             complement(5449..6249)
                     /locus_tag="ACHKNB_RS21910"
                     /old_locus_tag="pgaptmp_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGU4197774.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6490..7404
                     /gene="yafC"
                     /locus_tag="ACHKNB_RS21915"
                     /old_locus_tag="pgaptmp_004383"
     CDS             6490..7404
                     /gene="yafC"
                     /locus_tag="ACHKNB_RS21915"
                     /old_locus_tag="pgaptmp_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HGU4197775.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7401..8204)
                     /gene="dkgB"
                     /locus_tag="ACHKNB_RS21920"
                     /old_locus_tag="pgaptmp_004384"
     CDS             complement(7401..8204)
                     /gene="dkgB"
                     /locus_tag="ACHKNB_RS21920"
                     /old_locus_tag="pgaptmp_004384"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HGU4197776.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPKGLAPEWD"
     gene            complement(8367..8443)
                     /locus_tag="ACHKNB_RS21925"
                     /old_locus_tag="pgaptmp_004385"
     tRNA            complement(8367..8443)
                     /locus_tag="ACHKNB_RS21925"
                     /old_locus_tag="pgaptmp_004385"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8407..8409),aa:Asp,seq:gtc)
BASE COUNT         2291 a         2079 c         2087 g         2169 t
ORIGIN      
        1 ttaatgaatg agacaacaag cgagaatcac gcacttacgc cagaataaaa tcattgaaca
       61 gagaaatggt ggaattcaaa tacaaaaaaa ccgcaaaatt aaaaatcttg cggctctctg
      121 aactcatttt catgagtgaa tttggcggaa cggacgggac tcgaacccgc gaccccctgc
      181 gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac
      241 gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg
      301 tttgctgcaa aaatcgccca agccgctatt tttagcgcct tttacaggta tttatgcccg
      361 ccagaggcag cttccgccct tcttctccac cagatcaaga cgggcttcat gagctgcaag
      421 ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat
      481 tgttgcttca ccttgttgct gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg
      541 accaccggtc atcgccagat aaacttccgc aaggatctgt gcatcgagta atgccccgtg
      601 tagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg
      661 cttaccagga aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt
      721 ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa
      781 cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact catcggctac
      841 ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc
      901 ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg
      961 acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcacc
     1021 aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct
     1081 catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct
     1141 accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gcaatccagg
     1201 acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg
     1261 ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc
     1321 gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc gccaggggat
     1381 cactcagtgg atccatagct ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt
     1441 aaaaaatgtc gatctctggc aacgtcttga tgctgcattg gggcagcatc aaatcaaatg
     1501 ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg aactggcccg
     1561 tgctgcggca atgaatccca cactggaaga tacaggctat caagtcgaag tttaagcctg
     1621 tggtttacga tattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact
     1681 ctgtttcatc ggatttagcg ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa
     1741 gcaaccaagc gcaggaatat gtgcattcaa tagttttcca ccgtgtttgt tccacgggag
     1801 aacgtggaaa cggctggcgt gcagcacttc aaaattcaac aaagagagcc agtccagttg
     1861 ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt ttgcgcaata ccggcacaag
     1921 tttgcgtaat cccattaaac tgatgggatt gaagccacta atcaccagcc agccatcatc
     1981 aatcaatacc cgatcggctt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt
     2041 atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa gtgggtccgc
     2101 ctgtacctgg acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc
     2161 ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt
     2221 agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa
     2281 atcgccccag caatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac
     2341 cttccgtttc acactaagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac
     2401 aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac
     2461 gcagagccag tattaaacgc cattaccgcc aataactggc aaccggaggc catatttctc
     2521 acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa
     2581 attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt agtcaaagat
     2641 ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact
     2701 ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct
     2761 ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc acttaataag
     2821 ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac gttatcaaat
     2881 atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa
     2941 gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg
     3001 caaattaatg tttttttaag aacagaagat attgatttaa ttaatgtaat taatgaagaa
     3061 acattattgc aacaacctga agaacgtttt gcatggttaa ggtcaaagaa agataggttc
     3121 tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa
     3181 ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt
     3241 gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg
     3301 aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag
     3361 atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc
     3421 ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt
     3481 tacgggcaga gccgtatatg tactggatag ccgggcaagt taaaaaacgt aacatgccta
     3541 tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg
     3601 ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac
     3661 agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaaca
     3721 tgatgcagcg tctgaacaag atgtttgacg gcgactggct tctgaccgta gcggcttata
     3781 acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca
     3841 cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg
     3901 cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg
     3961 aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt tgaaatggcg aaggttgcag
     4021 atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgtg aaaggctcca
     4081 cgctgggcgc aagtggtccg caatacgtga tggtgccaaa gaagcatgca gatcaactgc
     4141 gtgaatctct cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata
     4201 cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca agtatcgctt
     4261 cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc
     4321 tgaagccagg ccaaagtttg acgattggtg caggcagtag cgcacagcga ctggcaaaca
     4381 acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac
     4441 gccacggcgt gaacatcaaa gatgtgatgc gctggaacag cgacactgcg aatctgcaac
     4501 caggcgacaa gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaacca
     4561 gataataaaa aggcaccgat tcccccggtg ccttttttat ttatgctgct ttatgtgctt
     4621 ctaccatgat gatatcactg gtgaaagagc catcattctg caaggcaaaa tacgttctca
     4681 cctctgtcga tgcgctctgt tggtaaatgc gaatagcgtc tactaacgct tctggcgtac
     4741 gcattctcgc gacccatgaa gaaaattcca gcggtaactt atctgtaatt aaattatcaa
     4801 ctatcagatt ggcttcattg attaacctca accactcacc gctggcgtag tttcgtacgt
     4861 gagaggtatc gcgtaatgct tctaccgtct gtaaccagat gtcgcgcact ggatgacccg
     4921 gagacattac gtccatcaca atcatcctac cgccaggttt caatatccta ttcacttctc
     4981 gcagtgctgc accaacatca tgccagtgat gggcagaata acggctgata acaatatcaa
     5041 atgcgttatc ggcaaatggc agactttcgg cataaccctg gcgggtggtg atatttttca
     5101 gttgccgggc ttcggcagct tgtgccacga catccagcat ttgggcagat aagtcatacg
     5161 ccaccaccgc gctcacgttc tgcgcagcga caaagctggc atgccctgct ccgcagccca
     5221 tatcaagtac acttgcatca ggataatcag ccagacgcac cgccaggcgc tgtaaatctc
     5281 gcccggatgc atgtacggta ctggttaaat actcactggc ctgcgagcta aactgctttt
     5341 ctacgtggtc atggtgggat tgtgttgtca ttgcgctgtc cttttgttgt ttgagtataa
     5401 aaggacagcg ccaaacgttg gtctgccctg tggcagacct gacatacctt atttatcagg
     5461 cttgccggga ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccaatac
     5521 agaagcttcg ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc ggccaaacgc
     5581 ccgacggcgc tgatcatcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg
     5641 atataacgcg ttcatccttc tacggctcca ggcattgaaa tctcctgcca taatgaccgg
     5701 gccgctgtgg tgagctatct gatcaccaat aggaagtaac tgcttactat agacatccac
     5761 gcccagactg aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa
     5821 tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa
     5881 cgggcagcaa tacactggat gcgccgccga aagggtcatt acgccagaag gatgttgtgg
     5941 cagtacgaaa gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtactaa
     6001 ctctggcgtt gtctgcgctt cctgcaataa caccagatgt gcatctttgc cgtagttctt
     6061 taataccgac aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat
     6121 acgctcttcg gtacttaacg gttctccggg tggtaatgcc tggccgatgc tcgcaaaaga
     6181 ccccggcggt aagatccttt ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt
     6241 ttttcgcact tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt
     6301 ctactgttat agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag
     6361 caagtgctta caccggtcag aattttgctc attatggaga acattaaatc gcatgacata
     6421 tcacgcatcc tgatggttta atgacaaata aattcaaaat atctcttcta ttttgcaaca
     6481 ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc
     6541 ggcagcttta gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggcg
     6601 gtgaaaaagc tggagatgaa acttggcgtt agcctgctta atcggaccac gcgacaactt
     6661 agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg
     6721 gcatcggcag aatcagaaat tatggagacg cgtaatacac cgcgtggact gttacggatc
     6781 gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt taattaagcc tttccgtgaa
     6841 cgctatccgg aagtcacttt gtcgctagtc tcctccgaaa cgattattaa tttgatcgaa
     6901 agaaaagtgg atgtcgcgat acgcgctggt acgttaacgg attccagctt acgtgccagg
     6961 ccgttattta acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg
     7021 aagccagaaa cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct
     7081 tccctcaata cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt
     7141 ttgtcatcca atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg
     7201 tgtttgtccg actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg
     7261 gcagataaag tgttgccagt ggcaatgcca ttcagtgcag tctattacag cgaccgtgcg
     7321 gtaagtacgc gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc
     7381 ggaggagctg tcagagaggc ttaatcccat tcaggagcca gacctttcgg gctaaccagg
     7441 cggtcgttgc aatccagggc ggcgatcgct tttttatctt tggcatcgag ctgtaaattt
     7501 tgtgccttaa gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa
     7561 ccttccccca tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgcg
     7621 atacgagcaa taacctcatc tttcagggcc ttaccatacg ccagcgtcat ataggaagta
     7681 atatggatgc cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag
     7741 agttcaatct ggttagtagc gatgttttca gcgccaacag cagcaatcgc cttttccatc
     7801 aatgggatcg tgaagttgga aataccgatc tcacgcgtca gcccttgttt tttggcttcc
     7861 agcagcgcct gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt
     7921 agcgtcagat caacataatc ggtacgcaat ttttgcaggc tctctttcag acttgggatc
     7981 aatttgtctt tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt
     8041 ggcacgccac tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgcg
     8101 gtatcaattg cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg
     8161 tctttcagac ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa
     8221 tgctattttc gttaactgag gtgattatgg gcttcagact gttgaggaaa aagagcgtaa
     8281 aatgcagagg atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga
     8341 gcgttaactc gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc
     8401 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa
     8461 ccaccggttt tatgaccggc ttactgctta atttgatgcc tggcagttta tgaatcactt
     8521 cgtgattcac ccttcgggcc gttgcttcgc aacgttcaaa agctttcgct tttgtcctac
     8581 tctcttatcg agtagtgaac tgcctcaaag atcagtgcca ttcttc
//
LOCUS       DAZFVO020000189         8623 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFVO020000189 DAZFVO020000000
VERSION     DAZFVO020000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.190"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..554
                     /locus_tag="ACHKNB_RS21930"
                     /old_locus_tag="pgaptmp_004386"
     CDS             <1..554
                     /locus_tag="ACHKNB_RS21930"
                     /old_locus_tag="pgaptmp_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HGU4197777.1"
                     /translation="DKFDNFMRQHRFSKVNFDTQQMIHFINRFNMNKGLIDKNNNFFI
                     QLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRDDNIFI
                     LFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPETLFGSKSQ
                     DKHALFPIFTASWRATRIKNKGI"
     gene            777..1274
                     /gene="ubiC"
                     /locus_tag="ACHKNB_RS21935"
                     /old_locus_tag="pgaptmp_004387"
     CDS             777..1274
                     /gene="ubiC"
                     /locus_tag="ACHKNB_RS21935"
                     /old_locus_tag="pgaptmp_004387"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HGU4197778.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSCKPLLLTELFLP
                     ASPLY"
     gene            1287..2159
                     /gene="ubiA"
                     /locus_tag="ACHKNB_RS21940"
                     /old_locus_tag="pgaptmp_004388"
     CDS             1287..2159
                     /gene="ubiA"
                     /locus_tag="ACHKNB_RS21940"
                     /old_locus_tag="pgaptmp_004388"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HGU4197779.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2314..4737)
                     /gene="plsB"
                     /locus_tag="ACHKNB_RS21945"
                     /old_locus_tag="pgaptmp_004389"
     CDS             complement(2314..4737)
                     /gene="plsB"
                     /locus_tag="ACHKNB_RS21945"
                     /old_locus_tag="pgaptmp_004389"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HGU4197780.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4908..5276
                     /gene="dgkA"
                     /locus_tag="ACHKNB_RS21950"
                     /old_locus_tag="pgaptmp_004390"
     CDS             4908..5276
                     /gene="dgkA"
                     /locus_tag="ACHKNB_RS21950"
                     /old_locus_tag="pgaptmp_004390"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HGU4197781.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5386..5994
                     /gene="lexA"
                     /locus_tag="ACHKNB_RS21955"
                     /old_locus_tag="pgaptmp_004391"
     CDS             5386..5994
                     /gene="lexA"
                     /locus_tag="ACHKNB_RS21955"
                     /old_locus_tag="pgaptmp_004391"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HGU4197782.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6067..7392
                     /gene="dinF"
                     /locus_tag="ACHKNB_RS21960"
                     /old_locus_tag="pgaptmp_004392"
     CDS             6067..7392
                     /gene="dinF"
                     /locus_tag="ACHKNB_RS21960"
                     /old_locus_tag="pgaptmp_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HGU4197783.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7508..7717
                     /gene="yjbJ"
                     /locus_tag="ACHKNB_RS21965"
                     /old_locus_tag="pgaptmp_004393"
     CDS             7508..7717
                     /gene="yjbJ"
                     /locus_tag="ACHKNB_RS21965"
                     /old_locus_tag="pgaptmp_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGU4197784.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7759..8274)
                     /gene="zur"
                     /locus_tag="ACHKNB_RS21970"
                     /old_locus_tag="pgaptmp_004394"
     CDS             complement(7759..8274)
                     /gene="zur"
                     /locus_tag="ACHKNB_RS21970"
                     /old_locus_tag="pgaptmp_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HGU4197785.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2005 a         2063 c         2320 g         2235 t
ORIGIN      
        1 atgacaagtt cgataatttt atgcgtcaac atcgattcag taaggtgaat ttcgataccc
       61 aacagatgat ccattttatt aacagattta atatgaataa agggctgatt gataaaaata
      121 acaatttttt tatccaactt atcgatcagg ccttacgatc aacggatgat atgatcaaag
      181 caaatgcctg gtatctttat aaagagtgga ttcgtagtga tgatgtttca cctatattta
      241 tagaaactga agaaaagtta agaaccttta acactaataa attaacacga gacgataata
      301 tatttatcct gttctcctct gtcgatgatg ggcctgttat ggtggtaagc tcccagcgct
      361 tacatgatat gttgaatcct acaaaagata ccaattggaa ttccacgtat atctacaaat
      421 ccagacatga gatattgcct gttaatctta ctccggaaac acttttcggc tccaaatctc
      481 aggataaaca tgcacttttc cccattttta ctgcgagttg gcgagctact cgtataaaga
      541 ataaaggtat ttaagtaaag gaaaacatca ccattcctgg catcatgggc ggtgatgccc
      601 ctatggttgc cctcgccagc acgggcatcg gcaaagcgta aggttcaaca gcgttttacc
      661 acttcatgcg attgttgcgt ttttgttgcg cattagatca cttaatttgc tttacatctc
      721 ccgtaaacac ttttctgcga tacaatgcct ttacgttatg taacggagag ttcggcatgt
      781 cacaccccgc gttaacgcaa ctgcgtgcgc tgcgctattt taaagagatc cctgccctgg
      841 agccgcaact gctcgactgg ctgttgctgg aagattccat gacaaaacgt tttgaacagc
      901 agggaaaaac ggtgagcgtg acgatgatcc gcgaagggtt tgtcgagcag aatgaaattc
      961 ccgaagaact gccgctgctg ccgaaagagt ctcgttactg gttacgtgaa attttgttat
     1021 gtgccgatgg tgaaccgtgg cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg
     1081 ggccggagct ggcgttacaa aaattgggta aaacgccgtt aggacgctat ctgttcacat
     1141 catcgacatt aacccgggac tttattgaga taggccgtga tgccggactg tgggggcgac
     1201 gttcccgcct gcgattaagc tgtaaaccgc tgttgctaac agaactgttt ttaccggcgt
     1261 caccgttgta ctaagaggaa aaaaatatgg agtggagtct gacgcagaat aagctgctgg
     1321 cgtttcatcg cttaatgcgt acggataagc caattggcgc gttactgctg ctctggccaa
     1381 cattatgggc gttgtgggtg gcgacaccgg gcgttcctca gctctggatc ctggcggtgt
     1441 ttgtcgcggg tgtctggctg atgcgcgctg ccggatgtgt ggtgaatgat tatgctgacc
     1501 gcaagtttga tggtcatgtt aagcgcacgg cgaaccgacc gcttcccagc ggcgcggtaa
     1561 cggagaaaga ggcgcgcgcg ctgtttgtag tgctggtatt gatttcattt ttactggtgc
     1621 tgacgctgaa tacgatgact attctgttgt cgattgccgc gctggcgctg gcgtgggtat
     1681 acccatttat gaagcggtat acccatctac cgcaagtggt gctgggcgcg gcgtttggct
     1741 ggtcgattcc aatggctttt gccgctgtga gtgagtcggt gccattgagt tgctggttaa
     1801 tgttcctcgc caatattctc tgggcggtgg cttacgacac gcagtatgcg atggttgacc
     1861 gcgatgatga tgtgaagatt ggcattaaat ccacggcaat cctgttcggc caatacgata
     1921 aattgattat tggtattttg cagattggcg tactggcact gatggcgatc atcggtgagt
     1981 taaatggctt aggctgggga tattactggt caattgtggt ggctggcgcg ctgtttgttt
     2041 atcaacaaaa actgattgcc aaccgcgagc gtgaagcctg ctttaaagca tttatgaata
     2101 ataactatgt tggtctggta ctatttttag ggctggcaat gagttactgg catttctgat
     2161 gatgtaaaaa agccggatga tcatccggct ttcttctggg ttgcctgatg cgcggcgctt
     2221 ctcaggccta cacaacacat cgcaatttat tgaatttgca gattatggaa ggccggataa
     2281 ggcgttttcg ccgcatccgg caattctctc tgattaccct tcgccctgcg tcgcactctc
     2341 aatcgtcaaa cgcacgtctg atgtaatcaa ctccgccagc aactgataaa ccttcatcgt
     2401 ttctgccggt tcggcatcgc cgctatcgct gatataccct tcatcacgca gtgtcagcac
     2461 cagagaactg aacaccgcct tgtcgaagaa ctccggcgcg ttgatgccgt gcagcacgga
     2521 gagacgttgc gcgacggtgc ggctctcttt ctccagcgta ccgcggttga tcgacgggtt
     2581 ggcactcaac aaccagaagg tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc
     2641 gagcagctgt agcgtgcgag aatgcaccgg gttgatatgc aactcatcat cttgcagggt
     2701 aatcagcccc tgacgttgca tctcatttgc cagcgcatca ataacgtccg gcaactcgtc
     2761 gcgatcccag cgcaggaaca gctccgcttt cagcattggg taaagcacat tgacgtgctc
     2821 catcaatacg tcgcgggaga tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg
     2881 cagcaccaac atatgcgcaa tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg
     2941 tggcagaatg atgatgtcgc cgatagtgtc tttctcgact tcaaacttgt tcatttgcag
     3001 cgcgtgatcg ataagctcgc tggcgctggc tgaaggaacg gtagagtccg tggagtaagg
     3061 tacgttgcgc atcagatcca ggtagcagtt gagttgctcg gttaactgct cgcgggtgag
     3121 tgagcgctga cgtgatgcca gtagcgcagt acagcacagg ttcatggcgt ttgccgcgcc
     3181 tgcgttgtta atgcgtacca tcagatcggc agcaatatta ttgaccgtcg gcgttaacca
     3241 ggccggacgc accgcttcga tgggatcgat agattcacgc cagtctggta cgtgctggtt
     3301 aaggtaggtc atcaacggca ttggttcacc gaagttgacg taaccctgac cgagattacg
     3361 cagcttgctt aaaccgcgca gcatctgcgg caggctctct ttctctttcg tcgcgccgcg
     3421 cagttctttg gcgtaagtac ccacttccat gacgtgctca taaccgatat agatcggaat
     3481 cagcgtaatc ggacgcgtgc cgccacgcag catcgcctga atggtcatcg acagcgtacc
     3541 ggttttcgga tccagcaaac gccccgtgcg ggaacgaccg ccttccacga agtactcgac
     3601 ggaataacca cggctgaaca gttcgccgag atactcacgg aaaacggtgg aataaagttt
     3661 attgccttta aaagtacggc gaataaagaa cgcccccaga cggcggaaaa tcggcccggc
     3721 cggccagaag ttcaggttga tcccggcggc gatatgcggc ggcaccagcc cctggtggta
     3781 cagcacgtaa gaaagtagca ggtagtccat gtgactgcgg tggcaaggca catacaccag
     3841 ctcatgaccg tcgtgggcca gctggcgaac gcgctcggcg ttatggacgt tgatgccctg
     3901 ataaagtcgg ttccaggtga agcccagaat gcggtcagtc aggcggatca tctcgtaaga
     3961 gaaattcgcc gcaatctctt ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga
     4021 gatttttttg ctgcgcgctt catcttctac cgctttggca atggcgcggg aggcgagcag
     4081 cttattaaac agatcctgac gagcaggcag acgtgggccc acagcagcca gacgttgacg
     4141 ggcaaagtgc atacgcgcca cgcgcgccag tttctgtgcg atagttttat ccgtgccgtg
     4201 ttcatccgcc atacggcgca gcgaaactga cggcgagaaa cgcacaaaac tgtcgcgacc
     4261 gagccacagt acggcgaaaa atttctgtac gccgttaagc atacgcagcg gcgggtttac
     4321 ttcgcctttt tcacgccccg gcgcgcgacc aaacatcacc gatactggca ccatctgcac
     4381 atccagattt gggttgctac ggtgcaaatc gagatagtcg tggaacagct taatagactc
     4441 ttctttcggc gtgtaatagg tgaatacacg cggcccgccg tgaatgaaca catagcgcgg
     4501 cagtagcgtg ccgtcgattt ccagcggctc taacgggtca ggcaagtcat gagccagaca
     4561 ctgggcgcgt aacgtcagca aatctgcttt cgagttgtac ggtaaaacgt acataattgg
     4621 acgagaagta tccagcccca gttccggggc aggatctgcc ggaatagact tgctttttac
     4681 caggatgctt aatggtaaat tcagtaattt gtagtaaatt cgtggccagc cggacataaa
     4741 cgatgtaaag cctctggtta ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg
     4801 aaattctgtg gtatccgctc atgtttcgcg cggcgttacg caaacccgaa tcatcggatt
     4861 taacggtaca ctgatattga cgctcataat gtaaaaaggt tctttcaatg gccaataata
     4921 ccactggatt cacccgaatt atcaaagctg ctggctattc ctggaaaggt ttacgcgctg
     4981 catggatcaa cgaagcggca ttccgtcagg aaggcgtagc ggtattgttg gcggtggtca
     5041 tcgcctgctg gctggatgtg gacgcaatta cccgcgtgct gcttatcagc tccgtgatgc
     5101 tggtgatgat tgtggaaatt ctcaatagcg ccatcgaagc agtggttgac cgaattggct
     5161 ctgaatacca tgagctttcc ggacgcgcta aagatatggg atccgccgcg gtgctgattg
     5221 ccattatcgt cgccgtgatt acctggtgca ttctgttatg gtcgcatttt ggataaccct
     5281 tccagatttc gataaatctc tggtttattg tgcagtttat ggttccaaaa tcgccttttg
     5341 ctgtatatac tcacagcata actgtatata cacccagggg gcggaatgaa agcgttaacg
     5401 gccaggcaac aagaggtgtt tgatctcatc cgtgatcaca tcagccagac aggtatgccg
     5461 ccgacgcgtg cggaaatcgc gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa
     5521 catctgaagg cgctggcacg caaaggcgtt attgaaattg tttccggcgc atcacgcggg
     5581 attcgtctgt tgcaggaaga ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt
     5641 gaaccgcttc tggcgcaaca gcatattgaa ggtcattatc aggtcgatcc ttccttgttc
     5701 aagccgaatg ctgatttcct gctgcgcgtc agcgggatgt cgatgaaaga tatcggcatt
     5761 atggatggtg acttgctggc agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt
     5821 gtcgcacgta ttgatgacga agttaccgtt aagcgcctga aaaaacaggg caataaagtc
     5881 gaactgttgc cagaaaatag cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc
     5941 ttcaccattg aagggctggc ggttggggtt attcgcaacg gcgactggct gtaacatatc
     6001 tctgagaccg cgatgccgcc aggcgtcgcg gtttgttttt catctctctt catcaggctt
     6061 gtctgcatgg cattcctcac ttcatctgat aaagcactct ggcatctcgc cttacccatg
     6121 attttctcca atatcaccgt tccgttgctg ggactggtcg atacggcggt aattggtcat
     6181 cttgatagcc cggtttattt gggcggcgtg gcggttggtg caacggcgac cagctttctc
     6241 tttatgctgt tgctgttttt acgcatgagc accaccgggc tgactgcgca ggcttatggt
     6301 gccaaaaatc ctcaggcatt agcccgtgcg ctggtgcaac cgttgctgtt ggcgttgggg
     6361 gctggggcgt taattgcgct gctgcgtaca ccgattatcg atctggcgct gcatattgtt
     6421 ggcggtagtg aagcggtcct ggaacaggcg cggcgctttc ttgaaatccg ctggttaagc
     6481 gctccggcgt cgctggcgaa tctggtatta ctcggttggt tactcggcgt gcaatatgcc
     6541 cgtgcgccag taattttgtt agtggtcggc aatatcctca acattgtgct ggatgtctgg
     6601 ctggtgatgg ggctgcatat gaacgtgcag ggcgcggcgc tggcgacggt tattgcggaa
     6661 tatgcaacat tgctgattgg tctgctaatg gtgcgtaaaa tcctcaaact acgcggaatt
     6721 tccggcgaaa tgctgaaaac tgcctggcga ggaaacttcc gtcgcttgct ggcgcttaac
     6781 cgcgatatca tgctgcgttc gctgttgttg caactctgtt tcggcgcgat caccgtactt
     6841 ggtgcgcgac tggggagtga cattgtcgct gttaacgcgg ttctgatgac gctactcacc
     6901 tttaccgcct atgcgcttga tggttttgcc tacgcggttg aagcgcactc cggtcaggca
     6961 tacggtgcgc gcgacggtag ccagttgctg gatgtatggc gggcagcgtg ccgccagtcg
     7021 gggattgtag cgttactgtt ttcggtggtt tatttgctgg caggggaaca catcattgcg
     7081 ttgctgacgt cgttaaccca gattcagcag ctggctgacc gctatcttat ctggcaggtg
     7141 attttgccgt tggttggcgt ctggtgttat ctgctggacg gcatgtttat aggcgcaacg
     7201 cgtgccgccg aaatgcgtaa cagtatggcg gtggccgccg caggttttgc gctgacgctc
     7261 cttacgctgc cgtggctggg taatcatggt ttgtggctgg cattaaccgt ctttctggcg
     7321 ttgcgcgggc tttctctggc ggctatctgg cggcgtcact ggcgcaacgg tacctggttt
     7381 gccgcaacgt gacggttaaa aattctgaat aaattatcct aagccaaatt gctgactaca
     7441 cttaatctca cgttcagaag aaaagtgaac gtactctcat tcacaaccta acgatgaggt
     7501 cttgattatg aataaagatg aagccggcgg taactggaaa cagtttaaag gtaaagtgaa
     7561 agagcaatgg ggcaaactga ccgatgatga tatgacgatc attgaaggta aacgtgatca
     7621 actggtcggt aaaatccagg aacgttatgg ttatcagaaa gatcaggcag aaaaagaggt
     7681 cgtggattgg gaaacccgca atgaatatcg ctggtaatta atccctcctg cccgacgtgt
     7741 acaaggatgt acgccctctt aacgcggttt ctttttcacc tgcacagagt gatcatgctg
     7801 gcactgttca ggatgacgac acgcttccac ttccacgcat gccgcacaga gcccatgtgc
     7861 ttcaatcaca ttatgccgca gggcaaaccc catttttgcc gccagcgtat gcataatgtc
     7921 ttccacgcct tctgcacact cttctttcac cgcgccgcag cgatcgcata taaacatggc
     7981 tgacgtatgc gtgggctggt cgaacagatg acagagcaca taactgttgg tggattccac
     8041 cttatgcaca aaaccttgct caagcagaaa atccagcgcg cgataaaccg ttggcggctt
     8101 ggcttgcggt tcagcttcgc gcagtaaatc aagcagatca taagcgctga tagcgccatc
     8161 ctgcagactc atcaggcgca acacttccag gcgctgtggg gtcaggcgca cattacgctg
     8221 cgcgcagatt ttttcagcct gcgctaataa ctcctgcgtt gtggtctttt ccatcgggca
     8281 cctcaaagtg ggggttaaga atgtccttac tttaccatgt tccaggaaaa acaccgagaa
     8341 cccttattgt tgccgtaatg ttgattttct gttttgtagg taaggtgttg tgttgccttg
     8401 tcgtaccatt atcaacacga taataattaa taattcattt ttaaataaat tgcttcaagt
     8461 taactcgcta tttatttaac tgggtttaaa cgcatacctg tagttcagaa attatgagcg
     8521 atttttgata gtgaccagat ggcaaaagaa ttataaggag gacacggtac gacaaaaaag
     8581 gtagatgaat catgttgaag ataatacccg gtgcaactgg ata
//
LOCUS       DAZFVO020000190         8559 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFVO020000190 DAZFVO020000000
VERSION     DAZFVO020000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.191"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            53..571
                     /locus_tag="ACHKNB_RS21975"
                     /old_locus_tag="pgaptmp_004395"
     CDS             53..571
                     /locus_tag="ACHKNB_RS21975"
                     /old_locus_tag="pgaptmp_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HGU4197786.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(616..2661)
                     /gene="dcp"
                     /locus_tag="ACHKNB_RS21980"
                     /old_locus_tag="pgaptmp_004396"
     CDS             complement(616..2661)
                     /gene="dcp"
                     /locus_tag="ACHKNB_RS21980"
                     /old_locus_tag="pgaptmp_004396"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HGU4197787.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     SYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2798..3544
                     /gene="ydfG"
                     /locus_tag="ACHKNB_RS21985"
                     /old_locus_tag="pgaptmp_004397"
     CDS             2798..3544
                     /gene="ydfG"
                     /locus_tag="ACHKNB_RS21985"
                     /old_locus_tag="pgaptmp_004397"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HGU4197788.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3633..4319
                     /gene="rspR"
                     /locus_tag="ACHKNB_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /gene_synonym="ydfH"
     CDS             3633..4319
                     /gene="rspR"
                     /locus_tag="ACHKNB_RS21990"
                     /old_locus_tag="pgaptmp_004398"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HGU4197789.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4497..4700
                     /gene="ydfZ"
                     /locus_tag="ACHKNB_RS21995"
                     /old_locus_tag="pgaptmp_004399"
     CDS             4497..4700
                     /gene="ydfZ"
                     /locus_tag="ACHKNB_RS21995"
                     /old_locus_tag="pgaptmp_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HGU4197790.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4736..6196)
                     /locus_tag="ACHKNB_RS22000"
                     /old_locus_tag="pgaptmp_004400"
     CDS             complement(4736..6196)
                     /locus_tag="ACHKNB_RS22000"
                     /old_locus_tag="pgaptmp_004400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HGU4197791.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6285..7568)
                     /gene="ydfJ"
                     /locus_tag="ACHKNB_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /pseudo
     CDS             complement(6285..7568)
                     /gene="ydfJ"
                     /locus_tag="ACHKNB_RS22005"
                     /old_locus_tag="pgaptmp_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            8172..8285
                     /locus_tag="ACHKNB_RS22010"
                     /old_locus_tag="pgaptmp_004402"
     CDS             8172..8285
                     /locus_tag="ACHKNB_RS22010"
                     /old_locus_tag="pgaptmp_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HGU4197792.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            8354..>8559
                     /gene="ydfK"
                     /locus_tag="ACHKNB_RS22015"
                     /old_locus_tag="pgaptmp_004403"
     CDS             8354..>8559
                     /gene="ydfK"
                     /locus_tag="ACHKNB_RS22015"
                     /old_locus_tag="pgaptmp_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HGU4197793.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLK"
BASE COUNT         2322 a         2140 c         1925 g         2172 t
ORIGIN      
        1 gcatacagaa aaagcaaatc acctttacat ataaaagcgt ggacaaaaaa cagtgaacat
       61 taatagcgat aaaattgtac aacttgcaga taccgacact attgaaaacc tgacatccgc
      121 gttgagtcaa agacttatcg cggatcaatt acgcttaact accgccgaat catgcaccgg
      181 cggtaagttg gctagcgccc tgtgtgcagc tgaagataca cccaaatttt acggtgcagg
      241 ctttgttact ttcaccgatc aggcaaagat gaaaatcctc agcgtaagcc agcaatctct
      301 tgaacgatat tctgcggtga gtgagaaagt ggcagcagaa atggcaaccg gtgccataga
      361 gcgtgcggat gctgatgtca gtattgccat taccggctac ggcggaccgg agggcggtga
      421 agatggtacg ccagcgggta ccgtctggtt cgcgtggcat attaaaggcc agacctatac
      481 tgcggttatg cattttgctg gcgactgcga aacggtatta gctttagcgg tgaggtttgc
      541 cctcgcccag ctgctgcaat tactgctata accaggctgg cctggcgata tctccaggac
      601 agccattggt ggtgtttata tgttcaagcc acgatgttgc agcatcggca taatctgagg
      661 agccttaccg cgccattgtc gatacaggcg ttccagatct tcgctgttac ctctggaaag
      721 gatcgcctcg cgaaaacgca gcccattttc acgcgttaat ccgccctgct caacaaacca
      781 ctgataacta tcatcggcca acatttgtgt ccacagataa gcgtaataac ctgcggcata
      841 tccgccacca aaaatatggg cgaaataact gctgcgatag cgtggcggta tagcaggaag
      901 atccatattt tccgccacca gcgcccgcaa ttcaaaatca tcgacatcct gcattgcttc
      961 gttttcttct aggcaatgcc agcgcatatc gagaagtgcg gcgctaagca gttcgctcat
     1021 ctcataccct ttgttgaaca ggctggcatt acgcattttc tgttgcagtt cgtcaggcat
     1081 tgctgccccg ctctgataat gccgggcgta gcgagcgaat acctgcggat gcgttgccca
     1141 gtgttcgttg atttgcgacg gaaattcgac aaaatcacgc ggcgtgttgg tgccggaaag
     1201 cgtggcataa cgctggcggg caaaaaggcc gtgcagcgta tgaccaaatt catggaataa
     1261 ggttatgaca tcatcccaga gtaacaacgc aggctcaccg gcagcgggtt tctgataatt
     1321 gcaaacgtta taaattaccg gatgcgtttc attaagcgtt gattgctcaa caaaattgcc
     1381 catccatgca ccgccgcttt ttgaatcacg ggcgaagaaa tcaccgtaaa ataacgccag
     1441 tcccacgcca ttatgatcaa aaatttccca cacacgaacg tcaggatggt agacaggaat
     1501 atcaaaacgt tcgacaaact taataccgaa gagctgattc gcggtccaga atacaccttc
     1561 attcaacacc gtgtttaatt caaaatatgg cttgagctgc gcctcatcaa gatcaaattt
     1621 ctcccgccgt acctgttcgg cataaaatgc ccagtcccac ggctgcgcgc taaacccgcc
     1681 ttgctgctta tcgataaccg cctgtatgga ggctaattca tcgctagcac gttgacgcgc
     1741 cgctggaaca atttcccgca taaagttgag tgctgcttca ggtgtttttg ccatctgatc
     1801 ggcgattttc catgcggcat aatgaggaaa accaagcagt ttcgcctgct gtgcgcgaat
     1861 ctctaccaga cgttgaatga tagcgcgggt atcattgcca tcattttttt ccgctcgcgt
     1921 ccagcccgca ataaacagtt tttcacgcgt cgcacgatcg cgcatttcgg caagcgccgg
     1981 ttgttgggtg gtattcagca gcggaatcag ccatttgtta tccagacctt tctcgcgagc
     2041 cgcctctgcc gccagcgcaa tctcttgctc actcattcct gccagctgcg cgatatcgtt
     2101 cacaaccaga ccgccggatt tatttgctgc aagtaaccgc tggttaaact ggctggtcag
     2161 ggtcgcagct tctgtattca gtacttttaa ttttgcttta tcagcttgcg caagtttggc
     2221 tccggcaagg acaaaacgtt gatgaataac ctccaccagg cggatggatt cactatcaag
     2281 ccccagggat tcacggcgct gccagacagc atctacccgc gcgaataatt caccgttcag
     2341 atagatatca ttagccagtt ccgccagttc agcggaaaac tgttcatcaa gacgctgtaa
     2401 ttcatcattg gtatgcgccg cagtcatcgc aaaaaagacg ctggtaacgc gggtaagtaa
     2461 ttctccgctt tgttccagtg ccagaatagt attgttgaaa tcaggcgttt gcgggttaag
     2521 cgcgatggca gcaatttctg cccgcttttg ctgcattccc tcatcgaatg ccgggcgata
     2581 gtgatgattg gcaatttgat caaaatgagg agccagatac ggcagtgtgc tttgcacaag
     2641 gaaaggattc attgttgtca ttttcttctc ctgaacgcga ggtgttccat agcgtaggct
     2701 tactgataac ggagtgcaat cttgcaaacc agtattaccc gctcttaagc atcacgtgct
     2761 atgttattga cacacaaaag cgttgaggaa cagtgagatg atcgttttag taactggagc
     2821 aacggcaggt tttggtgaat gcattactcg tcgttttatt caacaagggc ataaagttat
     2881 cgccactggc cgtcgccagg agcggttgca ggagttaaaa gacgaactgg gagataatct
     2941 gtatatcgcc caactggacg ttcgcaaccg cgccgctatt gaagagatgc tggcatcgct
     3001 tcctgccgag tggtgcaata ttgatatcct ggtaaataat gccggcttgg cgttgggcat
     3061 ggagcctgcg cataaagcca gcgttgaaga ctgggaaacg atgattgata ccaacaacaa
     3121 aggcctggta tatatgacgc gcgccgtctt accgggtatg gttgaacgta atcatggtca
     3181 tattattaac attggctcaa cggcaggtag ctggccgtat gccggtggta acgtttacgg
     3241 tgcgacgaaa gcgtttgttc gtcagtttag cctgaatctg cgtacggatc tgcatggtac
     3301 ggcggtgcgc gtcaccgaca tcgaaccggg tctggtgggt ggcaccgagt tttccaatgt
     3361 ccgctttaaa ggcgatgacg gtaaagcgga aaaaacctat caaaataccg ttgcattgac
     3421 gccagaagat gtcagcgaag ccgtctggtg ggtgtcaacg ctgcctgctc acgtcaatat
     3481 caataccctg gaaatgatgc cggttaccca aagctatgcc ggactgaatg tccaccgtca
     3541 gtaattttta tacccggcgt aactgccggg ttattgcttg tcacaaaaaa gtggtagact
     3601 catgcagtta actcactcac aagcaagaac gaatgaccgt cgaaacgcaa cttaatccca
     3661 cacagcctgt caatcagcag atttatcgta ttcttcgtcg cgacattgtc cattgcctga
     3721 ttgcaccagg cacaccgttg tcggaaaaag aagtttctgt tcgtttcaat gtgtcacgcc
     3781 agccggttcg tgaagccttt attaaactgg cggaaaacgg cctgattcaa attcgtccgc
     3841 agcgtggcag ctacgtcaac aaaatttcca tggcccaggt gcgaaacggc agttttatcc
     3901 gtcaggccat tgagtgcgcg gtggcgcgtc gggcggcgag catgattacc gaaagccagt
     3961 gctaccaact ggaacaaaat cttcaccagc aacgcattgc cattgagcgc aagcaactgg
     4021 atgatttttt tgaacttgat gacaacttcc atcaactcct gacgcagatt gccgactgtc
     4081 aactggcgtg ggataccatt gagaacctga aagcgaccgt tgatcgcgtg cgctatatga
     4141 gtttcgacca tgtttctcca ccagaaatgc tgttacgcca gcatcttgat attttctctg
     4201 ccctgcaaaa acgtgatggc gatgcggtag aacgtgcaat gacgcaacat ttgcaggaaa
     4261 tcagcgaatc cgtgcgccag atccgccagg aaaacagcga ctggtttagc gaagagtaat
     4321 tcatttactc tcatcccatc cgggatgaga ggcttttccc cccgccttac ggctcatgca
     4381 tgcatcaaaa aagatgtgag cttgatcaaa aacaaaaaat atttcactcg acaggagtat
     4441 ttatattgcg cccgttacgt gggcttcgac tgtaaatcag aaaggagaaa acacctatga
     4501 cgacctacga tcgtaaccgt aacgcaatca ccactggcag ccgtgttatg gttagcggca
     4561 ccggtcacac tggcaagatc ctgtcgattg atactgaagg tctgaccgct gagcaaatcc
     4621 gccgcggaaa aaccgtagtt gttgaaggtt gtgaagagaa actggcacct ctggacctga
     4681 ttcgtctcgg catgaactaa gtgtgtgaat gccgccgatg gcggcattgc tttttttact
     4741 tcacggaata tttcgccacg gtcgctttcg cgccatgcgc taataaagac aagtacgctt
     4801 ccgtcacttt tgcagtaaac aagctattgt ctggcaaatc atcaccaaag attgccttaa
     4861 tcgccagcaa tgactggacg cgcgctttcc cttcggcact actttgtaca gccttctgaa
     4921 taacaggtaa cagtgggtca ctgatttcta tcggatttcc ctgttcatca acaccaccga
     4981 cataacgcat ccaacccgcg acgcccagcg ccagcagatc gaacttgctg tcatgcgcca
     5041 gatgccagcg aacagaatcc aacatccgct gtggcaattt ctggctacca tccatcgcaa
     5101 tctgccaggt tcgatgacgt aacgccggat tgctatagcg ttcaattaat cggttagcgt
     5161 aatcttgcaa atcaacgccc tgcactttca gcgtcggcgc ttgttcctgc aacatcaagc
     5221 catacgccgc ataacgataa tgttcatctt ccatgcagtc attaatgtgc tgatatccgg
     5281 caagataacc caaatacgcc aggaatgaat gactgccgtt gagcatgcgc aacttcatct
     5341 cttcataagg cagcacatca ctaaccagtt cggctcccgc tttttcccat tctggacgtc
     5401 cggcaacaaa gttatcttct attacccact ggcggaaagg ttcacaggca acgcccgcag
     5461 gatcgcccac accggtaagt tgttcgattt tcgccagcgt atcctctgtc actgcgggca
     5521 caatacggtc caccattgtt gatgggaaag tcacgttatc ttcgatccat tgtgccagtt
     5581 ttacatcaac agctttcgcg tatgaagtga caacgtcacg cataacatga ccgttttctg
     5641 gcatgttgtc acatgacatg acggtaaatg cgggaagtcc tgccgcttta cggcgggcca
     5701 gcgcctcaac aatcacccct gttgctgttt tcggctggtg gggattttgc acgtcggcag
     5761 cgaccatcgg gtgatcgagc attaactgtc cggtcgccgg agagtggaaa tacccttttt
     5821 cggtgattgt cagagagaca atcgcgattt gcggttcaca catcgcagcc aacacggttt
     5881 ctaagccatc catctgtacg tgcaaggctt ttttaacgac gccaacgacg cgagccgtcc
     5941 acgcatcggc cgacatttcc gcaacggtat aaagattatc ttgctgttgt aaatcggcaa
     6001 tttgctgttc gccgccgatt aagttgacct cataatatcc ccagtcactg aaatgttctg
     6061 tagcaagaat atcggcatac acgccctgat gcgcacggtg aaatgcacca aagcctaaat
     6121 gaataattct tggggccagg ttattacgat cataaacagg gagtgtcgct tttgctgata
     6181 acaaattatt tcccataaca attccttaaa tttaaatatg gcaagctata tgttttgtta
     6241 tatgaataaa aatcccctct ccggtaagag aagggattga gggtttacag acttctggaa
     6301 ggttgcgcag ctcttacaac acgcggttga tcttccgcag cgtcttccag cgcacttaaa
     6361 tcacggtctt tcacctctgg cattttcagc gcagagatta aaccaattac tgaatatgcc
     6421 atgatcataa tggcgatcgg ataccaggat tccgtcatgg tgcagaaaat acccgccagg
     6481 ataggaccaa aaccggaagc gataatacca ccaatttctt tagaaatagc catccgggta
     6541 aagcggtttt tacagccgaa catttctgcc atggtaatgt tttccagagc aaataatccc
     6601 agcaccgcac agttatgaat cacaatcagt gcaaccataa tggtgctcgg ggcatagctt
     6661 ttatctacaa tgatagaaag cattggccat gccagcacaa tcgcggaggt attcataata
     6721 atatacggga tccggcgacc aattttatcg gataaccaac caaggaacgg aatggtcata
     6781 aagccgagaa tcgaactaat catcaatgca tctgttggaa ttgctttgtt aaacaataac
     6841 gtctgcacta aatagcctgc aaggaaagtc tgaattaacc cggagttacc cgcctgacca
     6901 aaacgcagcc ctgttgccag ccagaaggat ttgctctgga acatgctacc agcaggtgca
     6961 ggttttgctg tgggttggtt gctgtcatta accttctcaa agaccgggct ttctttcaga
     7021 ttcatacgta accagatagc aaagaccatc acgacaacgc tcgccaggaa cggtatacgc
     7081 catccccacg ccagcagttc ctctttactg agaatgaaga acataaaggc ccagattgcc
     7141 gttgcgctca aggttccgca gttagttccc atagctacaa atgaggagat aattccgcgc
     7201 ttaccttttg gtgcatattc cgccagcatc gtaccggcac cggaaatttc cgcacctgta
     7261 cccaacccct gaataatacg cagcgtcacc agcaagatgg gggcaaaaac accaatctgt
     7321 gcataggtcg gtaacacacc aattaaggtg gtacagatcc ccatcatggt gatggtaata
     7381 aagagcactt ttttacgccc tattctgtcg cccattttgc cgaaaataaa tgctccgaca
     7441 atacgcgcca cataacctgc accgtaggtt cccattgcca aaattaacgc cattgccgtt
     7501 gatgattcag gaaaaaatat ttcatgaaac actaacgctg cgccgagcga atataactgg
     7561 aaatccataa ttcacaggtg ttttttccca tcctgtggtt tccttggcgt tttctaggtt
     7621 ttttcagata gttgcatttt ttaaaaagca tcctaagttc gatctcagtg tctatctggg
     7681 gcctatttct gtcccatata tgccccaaaa aaactcccca acagataagt agttttttca
     7741 tggatttatg cgtaaaatca agaacggctg gaaatcattc aatactcaca ctatcgaaaa
     7801 atttaccagc caaccgcagc acgttcttgc ataaggcgtg tctgcggttt ttcaactatt
     7861 cagatacatc actcccatca cattcattcc tccgcatcaa aggcatatag gctatatcac
     7921 cttgatattt ttcttcttca gataaaaact gttatctatg tatactttta aacccaatcc
     7981 gtgtagagtc tctgcataag atagtttgca gttgccgctt cagcttgcgc cataaaccgc
     8041 ctgatttttg ctgccacctg ttagcattcc tgtatacctg aaacgacaat gtttatctac
     8101 gaactttaag aacacccaag ataaaaattg tcaactatat catatataac acattactaa
     8161 ttcgaggcta tatgaacagc atactgataa tcacttctct ccttatcata ttcagcattt
     8221 ttagtcatgc cctaataaaa ttagggattg gcatatccaa taacccagac aaaaccgatg
     8281 tataagtcaa catatcctga atcagacata caatatcgca atgaaaatca ataatatttt
     8341 aaggaatatc ttcatgaaat caaaagacac cctaaagtgg ttccctgcgc agcttcctga
     8401 agtaagaatt atcctagggg atgctgtagt ggaagtagca aaacagggaa gacctattaa
     8461 taccagaaca ttgcttgatt acattgaagg aaacataaag aaaaaatcat ggctggataa
     8521 caaagaatta ttacaaacag cgatatcagt tcttaaaga
//
LOCUS       DAZFVO020000191         8531 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFVO020000191 DAZFVO020000000
VERSION     DAZFVO020000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.192"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(204..1256)
                     /gene="fbaB"
                     /locus_tag="ACHKNB_RS22020"
                     /old_locus_tag="pgaptmp_004404"
     CDS             complement(204..1256)
                     /gene="fbaB"
                     /locus_tag="ACHKNB_RS22020"
                     /old_locus_tag="pgaptmp_004404"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HGU4197794.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYLGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1513..2790
                     /locus_tag="ACHKNB_RS22025"
                     /old_locus_tag="pgaptmp_004405"
     CDS             1513..2790
                     /locus_tag="ACHKNB_RS22025"
                     /old_locus_tag="pgaptmp_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HGU4197795.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2787..3791
                     /locus_tag="ACHKNB_RS22030"
                     /old_locus_tag="pgaptmp_004406"
     CDS             2787..3791
                     /locus_tag="ACHKNB_RS22030"
                     /old_locus_tag="pgaptmp_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGU4197796.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3788..4753
                     /locus_tag="ACHKNB_RS22035"
                     /old_locus_tag="pgaptmp_004407"
     CDS             3788..4753
                     /locus_tag="ACHKNB_RS22035"
                     /old_locus_tag="pgaptmp_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HGU4197797.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4727..5473)
                     /locus_tag="ACHKNB_RS22040"
                     /old_locus_tag="pgaptmp_004408"
     CDS             complement(4727..5473)
                     /locus_tag="ACHKNB_RS22040"
                     /old_locus_tag="pgaptmp_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4197798.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5525..6343)
                     /locus_tag="ACHKNB_RS22045"
                     /old_locus_tag="pgaptmp_004409"
     CDS             complement(5525..6343)
                     /locus_tag="ACHKNB_RS22045"
                     /old_locus_tag="pgaptmp_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGU4197799.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFLQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6408..7208)
                     /gene="thiD"
                     /locus_tag="ACHKNB_RS22050"
                     /old_locus_tag="pgaptmp_004410"
     CDS             complement(6408..7208)
                     /gene="thiD"
                     /locus_tag="ACHKNB_RS22050"
                     /old_locus_tag="pgaptmp_004410"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGU4197800.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7205..7993)
                     /gene="thiM"
                     /locus_tag="ACHKNB_RS22055"
                     /old_locus_tag="pgaptmp_004411"
     CDS             complement(7205..7993)
                     /gene="thiM"
                     /locus_tag="ACHKNB_RS22055"
                     /old_locus_tag="pgaptmp_004411"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGU4197801.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8028..8124)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8216..8488)
                     /gene="rcnR"
                     /locus_tag="ACHKNB_RS22060"
                     /old_locus_tag="pgaptmp_004412"
     CDS             complement(8216..8488)
                     /gene="rcnR"
                     /locus_tag="ACHKNB_RS22060"
                     /old_locus_tag="pgaptmp_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HGU4197802.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
BASE COUNT         1942 a         2231 c         2215 g         2143 t
ORIGIN      
        1 agtaaaaggt aaaaaaaaac ggcctcccga tagggaagcc gtagcaaagt gcgcgtgttt
       61 ttatgccgga tgcggtgtaa acatacgtgg cagtgctatg taggcatgat aagacgcttc
      121 cgcgtcgcat caggcatagg ttgccggatg cagtgtaaac gccttatccg tcctacgggg
      181 tggtgctgtt cgtatgaggc gtatcaggca atagtaattt tgctatcgag ataaacgtcc
      241 tgcacggcat taatcagttt cacgccgtca gccatcgatt tcttgaacgc cttacgtcca
      301 agaatcagcc ccattccgcc agcgcgtttg ttgataaccg cagtacgcac tgcatcgctg
      361 aggtcagttt caccgcccgc agcaccgccg gagtttatca acccagcccg acccatatag
      421 cagttagcta actgataacg caccagatca atcgggtttt cgctggtcaa tttgctgtaa
      481 acacgatcgt cggtgtaacc gtaattaatt gctttatagc cgccgttatt ttccgccatt
      541 ttttgtttga cgatatctgc accgatggtt gccgccagat ggtttgcctg accggtcagg
      601 tcggcggaaa catggtaatc aacgccatct ttcttaaagg cggagttacg caaataggcc
      661 cacagcactg tcaccatacc cagctcgtgc gcacgttcaa aagccgcaga aatttcttca
      721 atctggcgac gtgactcttc cgagcccaaa tagatagtcg caccaaccgc aaccgcgccc
      781 atgttgaacg cctgctccac gctggcatac agcgtttgat cgtaggtatt cgggtaactt
      841 agcgtctcgt tgtgattaag tttgacgagg aatggaatgc gatgcgcata acgccgcgat
      901 accgacgcca gcacgccgta agttgacgcc acacagttac agcccgcttc gatcgccagt
      961 tcaacaatgt ttttcgggtc aaagtagagc gggttagcag caaatgaagc tccggcagag
     1021 tgctcaacgc cctggtcaac cggcagaata gaaagatatc ctgtgccagc cagacgcccg
     1081 gtgttgtaca acgtctgcat attacgtaac accgctggcg ggcgattatt gtcaatcatt
     1141 acgcggtcta cgtagtcatg tccggggaga taaagctggt cagaaggaat tgtcatacaa
     1201 cggtgctgta aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc tgtcattact
     1261 atgctcccgt aaattccgat tggatatcgg ctatggattg tcctggcccg ccttttgcgg
     1321 gcaatcataa tcctggtcgt tacgggcgag tttttccatc ttttggatgt tttttcagcg
     1381 ttttctgttg gctcgattca tcagaaaaaa tgttagcgcg gtcaagtttt caccgcaaag
     1441 gtatttaaaa ggtattatta agtggtattg tcatcgcgta ccttacatta cctgtcatga
     1501 aggaattaaa agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt tgaatggttt
     1561 atctggggcg cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg ttttagtgcc
     1621 ggagaaattg gctggtcgta tgcctgcaca gccattgcgg cgatcctgtc gccaattctg
     1681 gttggctcca tcactgaccg ctttttctcg gcgcaaaaag tgctggcggt attgatgttc
     1741 gcaggcgcgc tgctgatgta tttcgctgcg caacagacca cttttgccgg gttcttcccg
     1801 ttactgctgg cctactcgct aacctatatg ccgaccattg cgctgactaa cagtatcgct
     1861 tttgccaacg tgccggatgt ggagcgtgat ttcccgcgca ttcgtgtgat gggcactatc
     1921 ggctggattg cctctggtct ggcatgtggt ttcttgccgc aaatgctggg gtatgccgat
     1981 atctcaccga ctaacatccc gctgctgatt accgccggaa gttctgctct gctcggtgtg
     2041 tttgcgtttt tcctgcccga cacgccgcca aaaagcaccg gcaaaatgga cattaaagtc
     2101 atgctcggcc tggatgcgct gatcctgctg cgcgataaaa acttcctcgt ctttttcttc
     2161 tgttcattcc tgtttgcgat gccactggcg ttctattaca tctttgccaa cggttatctg
     2221 accgaagttg gcatgaaaaa cgccaccggc tggatgacgc tcggccagtt ctctgaaata
     2281 ttctttatgc tggcattgcc gtttttcact aaacgctttg gtatcaaaaa ggtattattg
     2341 cttggtctgg tcaccgctgc gatccgctat ggcttcttta tttacggtag tgcggatgaa
     2401 tatttcacct acgcgttact gttcctcggt attttgcttc acggcgtaag ttacgatttt
     2461 tactacgtta ccgcttacat ctatgtcgat aaaaaagccc ccgtgcatat gcgtaccgcc
     2521 gcgcaggggc tgatcacgct ctgctgtcag ggcttcggca gtttgctcgg ctatcgtctt
     2581 ggcggtgtga tgatggaaaa gatgttcgct tatccggaac cggtaaacgg gctgactttc
     2641 aactggtccg ggatgtggac tttcggcgcg gtgatgattg ccattatcgc cgtgctgttc
     2701 atgatttttt tccgcgaatc cgacaacgaa attacggcta tcaaggtcga tgatcgcgat
     2761 attgcgttga cacaagggga agttaaatga aaacagaacg tattctcggt gctctttatg
     2821 ggcaggcgtt aggggatgcg atggggatgc cctccgagct ttggccacgc agccgcgtta
     2881 aagcacactt tggctggatt gaccgttttc tgcctggacc aaaggagaat aacgcggcct
     2941 gttattttaa ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt ctggcggatg
     3001 cgttactgga acgtgaaggc aagatcgatc cggatctgat tgggcgtaat attctcgact
     3061 gggcgctgcg tttcgacgcc tttaacaaaa acgtactagg tccgacctcg aagattgcgc
     3121 ttaacgccat tcgcgacggt aaacccgttg ctgaactgga aaacaacggc gtgaccaacg
     3181 gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc ggcgcgtgat gttgattcct
     3241 ttattgatga tgtggcgctg gcgtcgagcc cgacccataa atccgatctg gcagttgcag
     3301 gtgcggtagt catcgcatgg gcgatttctc gtgccattga cggagaaagc tggtcagcga
     3361 ttgttgattc cctgccttca attgcgcgac atgcacagca aaaacgcatc actaccttca
     3421 gcgcctcact ggcagcacgt ctggagattg cgctgaaaat tgtgcgcaat gccgacggca
     3481 ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc aggtaccagc actattgagt
     3541 ccgttccgtg cgccattgcg ctggttgaac tggcacaaac cgacccgaat cgctgcgccg
     3601 tcctgtgcgc taaccttggc ggcgacacag acaccatcgg cgctatggcg acggcgattt
     3661 gcggcgcgtt gcatggcgtt aacgctatcg atcctgcatt aaaggcggaa ctggatgcgg
     3721 taaatcagct tgatttcaac cgctatgcca cagcgctggc gaaatatcgt caacaacggg
     3781 aggcggtatg agcggcgctc gattacacac gctgctgcct gaattaacct cgcgtcagcc
     3841 ggtgatggtc gtcggcgcgg cggtcattga tgtgatcgcc gacgcttatg ccctcccctg
     3901 gcgtgggtgc gatatcgaac tgaagcagca gagcgttaac gttggcggct gcgcactgaa
     3961 tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt aatgccttgc cgctcggtca
     4021 gggcgtgtgg gcggagatta ttcgcaaccg gatggcgaaa gaggggttaa tcagcctgat
     4081 cgataacgcc gaaggcgata acggctggtg tctggcgctg gttgagccgg atggcgaacg
     4141 cacttttatg tcattcagcg gtgtggaaaa tcagtggaat cgccagtggc tggcgcgatt
     4201 aaccgttgcg cctggcagcc tgctctattt ttccggttat caactggcct cgccctgcgg
     4261 cgaattgtta gtggaatggc tggaaaagct gcaagacgtg acgccgttta tcgattttgg
     4321 cccacgtatc ggcgatatcc cggacgcatt actggcgcgg atcatggcct gtcgaccttt
     4381 agtatcactc aatcgtcaag aggctgagat tgccgccgaa cgttttgctt tatccgcaga
     4441 gataacaaca cttggcaagc aatggcagga gaaatttgcc gcgccgttga tcgttcgtct
     4501 cgataaagag ggtgcatggt atttcagcaa cgacgcttct ggctgcattc cggcatttcc
     4561 aacgcaagtt gtagacacca ttggggcggg cgacagtcat gcaggtggcg tacttgccgg
     4621 gctggcctct ggtctgccac tggcggatgc cgtattactg ggcaatgcag tggcgtcgtg
     4681 ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc gaggaactac tcctcgcaca
     4741 caaaaacgta tagatcgctg cgacagtggc taatgctgta ctcaataggc cgttgctgtt
     4801 ggtcaagcgc cacttgcttg atcaccagca ccggtatttt gctatctaac tgaatatgtg
     4861 actgaaactc ggcatccggc atccgggcgc taacgcggga acgcgtacgt tgtgggtaaa
     4921 tgtgctggct gcggaaatag tcataaagtg aaatcccgat ggcatcaaca tcatgaatta
     4981 aatgcgccgg aacccacgat tcctcaatcg ataccgcttc ttcatcgaca tagcgaatac
     5041 gcttaagcaa aaagacatca cttcctgctt cgaccgccag ctgctgcgcg acttcttcgg
     5101 gacatttcac gacacgcttg ttaacccata gcgtatcggg ctttttcccg cgcaatacca
     5161 cctgctgaga aaaacctcgc gcttctttca acgagtattc gaagatgttg ttgatctgcg
     5221 tgccgtaacc gcgcgaacgc gtcaccacac cctcttcttc cagcgcctgc atcgccttgc
     5281 gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc acgctcaccg ggcaaaatat
     5341 tgccatgctc cagcaccccg ctgcgcacgg cattttttac cgtttcggca aacttaatat
     5401 aaagcggcgt gttatccgcc gctaaaatac gctcgttcag ttgggcaatc agctgggtat
     5461 gcgcttgttc catttgtctt tttcctgccg aggagtctgg tgggagtata tcactgcttc
     5521 ttatttaagg cgtttcttta atcccatcaa cgaatgcctg caactcctcc accgtgccat
     5581 gaaatacatt aaaatccacc ggaccattga tgccatctac ctgtccacgg tcggaatgct
     5641 gccagaagcg ccaggccatg ccgtcattgt ccggacgacg ttgataatag tgagccaccc
     5701 accacggata ttcattgaaa tagcccgcca gattggtgtg ataaaaaacg gctcctgagt
     5761 aaataatcgg cttttttccc gtactttttt cgaccatttt tagccactga cttactcgtt
     5821 tgcgtaattc ttttgccgat aattttcccc gttcttctac gtccagtacc gcagggaaat
     5881 cgccttgtaa gaaatccacc gtttgcagaa ataatcgtgc ctgaaccggc gctgctaccg
     5941 acggggagaa ataatgatac gccccgcgca gcaggccatt ttcgcggctt agttgccagt
     6001 tacgcgaaaa atacgggtcc accagctttt cgccttccgt cgccttaata aaagcaaact
     6061 gtaagcggat accgttgtcg cgcatttttg ccacccgctg ccagtcgatc cgctcctgcc
     6121 agcgagaaac atctattccg tgaatggtgt aactggcagg aatgcgaata gcgaaagatt
     6181 taaccgggcg ataaccgtag aaatggatgt agtctgtaat ctgaactgct gtcgaataga
     6241 aaaaattcac cgtctgacgc ggataaattg caacgatgga aattagccca agagcgcata
     6301 atagagaggt gagttttttt cgactggtaa ttcttagctg cattggtttc atccctgaat
     6361 gtcagtgcca aaggctgaca ataaccaaag cagctatagt acggtgctca ccaccaggcg
     6421 tggaagtggt gaaccggacc aataccgtga ccaacttcca gcgtgtcggc ctgggctaat
     6481 gctgatgaaa gccagctttt tgcctcctgt acggtgtcag cccagtttgt atggcgcggg
     6541 cgtagtgcag ccaacgccgc agagagcgta caaccagtgc cgtgggtgtt tttggtcata
     6601 atgcgcggtg cggtaaaccg ttgttcaccc tcgcgggtaa acagccagtc cgggctttgc
     6661 tcatcatcca gatgaccacc tttcattagc actgcgccac agcccatcgc caacagcgat
     6721 cgcccttgtt ccagcatttc ctgttcggtg cgcgcgtgtg gcgcgtcgag caaggcggca
     6781 gcttcgggca agtttggcgt tattaatgaa acctgtggca ataatcgact gcgcagcgta
     6841 gcaaccgccg aaggtgaaag cagcgggtcg ccgctttttg ccagcataac ggtgtcgagt
     6901 accacgtttt ggatctgata acgttgcaac cgttctgcca ccgcttcaac aatatcggtt
     6961 tccgccaaca taccgatttt agtggtgtcg attcgcacat cgctgaacac cgaatcgagc
     7021 tgggcggcga caaaatcagg ctcaatgcga tacaccgact gtacgccacg ggtattttgc
     7081 gccaccagtg cagtaataac tgagcaacca taagcgccaa gtgccgagaa ggttttaaga
     7141 tcggcctgaa tccccgcacc accactcgga tcagtaccgg caatcgtcag agcgttaatt
     7201 cgtttcatgc ctgcacctcc tgcgtcaatt gccagagcgc atcaaggaaa tgtggaacaa
     7261 aactgcctgg cccctcgctt ctggcgactg cgcgctctcc ggcttgtttc atccagtgac
     7321 aggcagatgc gacattttcc agcatatcgc ctggtaaggc acaacaggca gcaacaaccg
     7381 ccgataatgc acagccagtt cctaccactt tggtcattag cggatcgcca ccgtgaatgc
     7441 caacgatacg atgcccatcg gtaacataat ccacctcgcc tgtgaccacg acgattgcgc
     7501 cagtttcccg tgccagtgtt tgtgcagcgg gtatcgcgtt agctgcggcg tcagtggtat
     7561 ccactccccg tccgccatta gcaacgccag ctaatgccat gatttccgaa gcattaccac
     7621 gtatcgctgt cggtttaaaa gataaaaatt catgacaaaa atggcggcga taatcgagcg
     7681 caccaaccgc tactggatca agcgtccagg gtgtttgaga gctttttgct tgctcaacgg
     7741 cagcacgcat cgcctgtgcg cgtggctgcg tcagtgtgcc aacgttaatc aacaaggcac
     7801 tggcgatagc cgcaaactga ctggcctctt cggtttcgat aaccatcgct ggcgatgcac
     7861 cgagcgccag caaggtattg gcagtaaagg tttgcaccac atcgttggtc atgcagtgca
     7921 caagagggga atgttggtga aaaaggtgta acgcgtgcgc agattgcgct gaactcagca
     7981 ggtcgacttg catagtttgc tcctgccata acgtgaagaa gcaatgacct ggtggtccgt
     8041 gacttcccta cgctggcatt atccagatca ggtgatacgg gtatttctca gccttcacgc
     8101 agaagggcac cccgagtcgt ttggttgcga tgataaatcg cagaggagga tggtaatgtc
     8161 cagcgcacgc gttgtaaacg aggaaaaacg gcagttttac aatcgcgtta ataaattatt
     8221 tgatatatga atccagcacc ttcagaacga catccagatc ttcttcacgt tttagctcat
     8281 ccccctggtg aacgatgtgt tccgtcagat gacctttaat cacttcccgc atcagaccgt
     8341 ttaccgcgcc acggatagca gcaatctgtt gtaaaactgc agcgcattcg tgcggctcgt
     8401 cgagcatttt cttgagcgcc acgacctggc cctgaatctt actggcacgc gctttcagtt
     8461 tctgtttatc acggattgta tgagacatgg caacacctgg ttaacaagaa tatgaaaaat
     8521 catagcacta t
//
LOCUS       DAZFVO020000192         8399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFVO020000192 DAZFVO020000000
VERSION     DAZFVO020000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.193"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            311..1651
                     /gene="dcuB"
                     /locus_tag="ACHKNB_RS22065"
                     /old_locus_tag="pgaptmp_004413"
     CDS             311..1651
                     /gene="dcuB"
                     /locus_tag="ACHKNB_RS22065"
                     /old_locus_tag="pgaptmp_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HGU4197803.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1729..3375
                     /gene="fumB"
                     /locus_tag="ACHKNB_RS22070"
                     /old_locus_tag="pgaptmp_004414"
     CDS             1729..3375
                     /gene="fumB"
                     /locus_tag="ACHKNB_RS22070"
                     /old_locus_tag="pgaptmp_004414"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HGU4197804.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDKLPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRKGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3498..4127
                     /locus_tag="ACHKNB_RS22075"
                     /old_locus_tag="pgaptmp_004415"
     CDS             3498..4127
                     /locus_tag="ACHKNB_RS22075"
                     /old_locus_tag="pgaptmp_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HGU4197805.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(4266..5687)
                     /gene="melB"
                     /locus_tag="ACHKNB_RS22080"
                     /old_locus_tag="pgaptmp_004416"
     CDS             complement(4266..5687)
                     /gene="melB"
                     /locus_tag="ACHKNB_RS22080"
                     /old_locus_tag="pgaptmp_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HGU4197806.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(5790..7145)
                     /gene="melA"
                     /locus_tag="ACHKNB_RS22085"
                     /old_locus_tag="pgaptmp_004417"
     CDS             complement(5790..7145)
                     /gene="melA"
                     /locus_tag="ACHKNB_RS22085"
                     /old_locus_tag="pgaptmp_004417"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGU4197807.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            7428..8336
                     /gene="melR"
                     /locus_tag="ACHKNB_RS22090"
                     /old_locus_tag="pgaptmp_004418"
     CDS             7428..8336
                     /gene="melR"
                     /locus_tag="ACHKNB_RS22090"
                     /old_locus_tag="pgaptmp_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HGU4197808.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         2157 a         2217 c         2055 g         1970 t
ORIGIN      
        1 atatctgccc tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa
       61 acattaacat ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa
      121 aaagccgctt atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg
      181 aggcatttgc taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt
      241 catacaaaac agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga
      301 gggttcacac atgttattta ctatccaact tatcataata ctgatatgtc tgttttatgg
      361 tgccagaaag ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt
      421 cttcgtcttc caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc
      481 ggtggtggcg gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat
      541 tgccgagaag ttgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac
      601 ctgtacactg accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat
      661 ctacgacgtc gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat
      721 cggtgcacag atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc
      781 aatgctgggt aatgtcacct ttgatggtcg ccatcttgag ttcctcgacc tgctggcaat
      841 caccattcca tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg
      901 taaagatctg gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga
      961 gtatgtttac ggtgataccg cgacgctgct cgataaaaaa ctgccgaaaa gcaactggct
     1021 ggcaatgtgg attttcctcg gtgcaatcgc tgtagtcgcc cttcttggtg ctgattcgga
     1081 cctgcgtcca tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat
     1141 gctgctgacc ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa
     1201 aaacgaagtc ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat
     1261 ggcagaaacc atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt
     1321 gaaagagtat ccgtgggcct atgccattgt tctgctgctg gtttccaagt ttgtaaactc
     1381 tcaggctgcg gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc
     1441 atacatcgtg gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag
     1501 cgatctggca gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat
     1561 caaccacagc tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg
     1621 gatcttcgcc gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg
     1681 cggcacgcca ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa
     1741 ccctttatct accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc
     1801 acttccgatt acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca
     1861 gaagccctga ccctgctggc gcagcaagcc tttcacgacg cttcttttat gctccgcccg
     1921 gcacaccaga aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag
     1981 tacgtggcgc tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc
     2041 tgccaggata ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggc
     2101 ggcggtgatg aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg
     2161 cgctattcac agaatgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg
     2221 cctgcgcaaa tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg
     2281 aaaggcggcg gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact
     2341 cccggcaaac tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc
     2401 ccgccgtacc atatcgcgtt tgtgattggc ggtacgtctg cggaaaccaa cctgaaaacc
     2461 gtcaagttag caagcgctca ctattacgat aaactgccga cggaagggaa cgaacatggt
     2521 caggcgttcc gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt
     2581 cttggcgcgc agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca
     2641 cgtcacggcg catcctgccc ggtcggcatg ggcgtctcct gctccgctga ccgtaacatt
     2701 aaagcgaaaa tcaaccgcaa aggtatctgg atcgaaaaac tggaacacaa cccaggccag
     2761 tacattccac aagaactgcg ccaggccggt gaaggcgaag cggtgaaagt tgaccttaac
     2821 cgcccgatga aagagatcct cgcccagctt tcgcaatacc cggtatccac tcgtttgtcg
     2881 ctcaccggca ccattatcgt gggccgagat attgcacacg ccaagctgaa agagctgatt
     2941 gacgccggta aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg
     3001 gcgaaaaccc ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggccgtatg
     3061 gactcctacg tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggt
     3121 aaccgcagtc agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc
     3181 atcggcggtc cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct
     3241 tatccggagc tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt
     3301 atcctggtcg atgacaaagg taacgacttc ttccagcaga tcgtcaacaa acagtgcgcg
     3361 aactgcacta agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc
     3421 tgggccacct cttttgcgat tgtaatttca cgcttgctgg tgaatagtca gtattttccc
     3481 tgacttgcga actcaccatg acccgcacac tcaagccgtt aattcttaac accggcgcac
     3541 tggcgctaac gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc
     3601 tgatggaagt gacaccggtg attattgtcg tgccgctact ccttgccacc gcgaaacgtt
     3661 atccgttaac gccgctgctc tatacgctca ttttctttca cgccatcatc ctgatggtcg
     3721 gcggacaata cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggttagggt
     3781 tgagccgtaa tccgtatgac aagctggggc attttttcca ggggctggtg cctgcactgg
     3841 tggcacgaga aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgttcc
     3901 tggtctgctg cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg
     3961 cgctggcaat gggccaggga gcggatgatt ttctcggcac ccagggcgac cagtgggaca
     4021 cgcaatccga tatgttctgc gcgctgcttg gcgcattaac gacggtgata ttcctcgctc
     4081 gctttcattg ccgccagcta cggcgctttg gcttgataac cggataacaa cgtgcgcttt
     4141 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
     4201 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
     4261 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
     4321 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
     4381 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
     4441 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
     4501 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
     4561 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
     4621 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
     4681 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
     4741 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
     4801 cgtcgggata atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct
     4861 cccgcatacg acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa
     4921 tagatagcaa atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag
     4981 aggcatgaaa gctgatcgtt tttataaatt agcgcaacga tggctttaag tgtcagatgg
     5041 cttccttcag cagacggttg attgtctgac gaaaagactt catgcacatt gcgcagagtg
     5101 atgatggttg aaacaataaa aaaggcgatc agtaccagag tgaacatctg aaagccaaat
     5161 ccccgatcgc caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag
     5221 cctgccagac tggcaaaaaa acgcggataa ggaaccagtt gttcgcgctc acgtttatcg
     5281 agcgtgatgg ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc
     5341 cagaggatgt aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca
     5401 ctaaagagga gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg
     5461 aacttacccc atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata
     5521 gcatcccaga tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg
     5581 acatcggtgt agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg
     5641 aacgctccaa atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc
     5701 gctgttacca acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat
     5761 cccccggcgc agtagcgttt agtcgcgttt taacggtgca accagcctgg cagccagtcg
     5821 ccgtgggcgg caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg
     5881 gcagtatgcg ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata
     5941 gcttcggtca gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa
     6001 ggtagcgtac cgactttggt tggctgaatg ccattagcat caaccagaca ggctacttcc
     6061 acgcaacatc cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc
     6121 acactcggct cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt
     6181 ttaatatcaa tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc
     6241 tgctcgacgc agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc
     6301 tcacgacctg gcttaataaa ccacggcgta tattcggcaa aatgttctga cgattccgtg
     6361 acgaagtagc ccagcttttt gaacatttca tagcgcacaa tattctggca gcgagtatta
     6421 ccatgaatat ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag
     6481 agattcacgt aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg
     6541 ttgatacctg cgcagcggta acgcagcgta gctgggtcga tattgagatc acgcgccagc
     6601 tcttccgccg ttccctgcac cgaatggcac agcccgacct gtttgatatg cggataacgg
     6661 gcatacatcg cccaggtatt catcgccatg gggttaacat agttgagcat ggtggcatcg
     6721 gggcagactt ccgtcatgtc ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg
     6781 cgcataatac cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta
     6841 cagacctcga aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg
     6901 acaaaatcgg catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg
     6961 gcccctgctg aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtggga
     7021 tcaatgtcca tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca
     7081 agaatatttt taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagtc
     7141 atcatgcaga tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa
     7201 acctccggct tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa
     7261 atctgagttt atggtaataa tttacctgaa aagcagagtt ttcggcctca tgatgctcgg
     7321 acgctcccct cgcctctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct
     7381 ccctcactcc tgccataatt ctgatattcc aggaaagaga gccatccatg aatacagata
     7441 cgtttatgtg cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat
     7501 accagcggat ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg
     7561 gtcaggtcga agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa
     7621 aagtgaatat caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa
     7681 cagataccgg aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct
     7741 cctggccgct ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac
     7801 tggccacaca gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa ttaaacagtc
     7861 cgaacgagca aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta
     7921 gcctctctgg ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg
     7981 tctcgcgcca tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg
     8041 atcaggcgct gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa
     8101 tggggatatt ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca
     8161 tcaaccacgt tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga
     8221 cggcaggctt tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt
     8281 cgccgcaaca ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac
     8341 gatatcagta tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataatt
//
LOCUS       DAZFVO020000193         8397 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFVO020000193 DAZFVO020000000
VERSION     DAZFVO020000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.194"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..187
                     /locus_tag="ACHKNB_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /pseudo
     CDS             1..187
                     /locus_tag="ACHKNB_RS22095"
                     /old_locus_tag="pgaptmp_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502670.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(550..1743)
                     /gene="tyrB"
                     /locus_tag="ACHKNB_RS22100"
                     /old_locus_tag="pgaptmp_004420"
     CDS             complement(550..1743)
                     /gene="tyrB"
                     /locus_tag="ACHKNB_RS22100"
                     /old_locus_tag="pgaptmp_004420"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HGU4197809.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHIAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1996..3075)
                     /gene="alr"
                     /locus_tag="ACHKNB_RS22105"
                     /old_locus_tag="pgaptmp_004421"
     CDS             complement(1996..3075)
                     /gene="alr"
                     /locus_tag="ACHKNB_RS22105"
                     /old_locus_tag="pgaptmp_004421"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGU4197810.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(3128..4543)
                     /gene="dnaB"
                     /locus_tag="ACHKNB_RS22110"
                     /old_locus_tag="pgaptmp_004422"
     CDS             complement(3128..4543)
                     /gene="dnaB"
                     /locus_tag="ACHKNB_RS22110"
                     /old_locus_tag="pgaptmp_004422"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGU4197811.1"
                     /translation="MAGNKPFNRQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4626..5609
                     /gene="qorA"
                     /locus_tag="ACHKNB_RS22115"
                     /old_locus_tag="pgaptmp_004423"
     CDS             4626..5609
                     /gene="qorA"
                     /locus_tag="ACHKNB_RS22115"
                     /old_locus_tag="pgaptmp_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HGU4197812.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDYLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5775..6017)
                     /gene="pspG"
                     /locus_tag="ACHKNB_RS22120"
                     /old_locus_tag="pgaptmp_004424"
     CDS             complement(5775..6017)
                     /gene="pspG"
                     /locus_tag="ACHKNB_RS22120"
                     /old_locus_tag="pgaptmp_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HGU4197813.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRDDRWRY"
     gene            complement(6151..7188)
                     /gene="dusA"
                     /locus_tag="ACHKNB_RS22125"
                     /old_locus_tag="pgaptmp_004425"
     CDS             complement(6151..7188)
                     /gene="dusA"
                     /locus_tag="ACHKNB_RS22125"
                     /old_locus_tag="pgaptmp_004425"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HGU4197814.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7552..8264)
                     /locus_tag="ACHKNB_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /pseudo
     CDS             complement(7552..8264)
                     /locus_tag="ACHKNB_RS22130"
                     /old_locus_tag="pgaptmp_004426"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
     gene            complement(8288..>8397)
                     /gene="yjbL"
                     /locus_tag="ACHKNB_RS22135"
                     /old_locus_tag="pgaptmp_004427"
     CDS             complement(8288..>8397)
                     /gene="yjbL"
                     /locus_tag="ACHKNB_RS22135"
                     /old_locus_tag="pgaptmp_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HGU4197815.1"
                     /translation="DYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         2225 a         2129 c         1953 g         2090 t
ORIGIN      
        1 atcaagttct attgcaagta aagtttttca cgcatttatt gatttagtta acaaaatcag
       61 gggtaagaaa gactttatgt atatttgcac gctttgctgt tttgccgagg aggttaaagg
      121 tgattattct cattacagga cctttttatt tgatattggc aatcaatata aggttaaact
      181 tacacaaagc ggaaaaaaag agctctcttt aactttagaa tttaacgata ctataattga
      241 atctcagaaa gtcacaggca ataaggcaaa gcatattctt gaggatatag aaaaattcta
      301 tcgtaacaaa cccgatactt attattaaat ttaataatag ttagatgtgc agaaattatt
      361 actccaccca aaaaaacagg ataaggaatt ccagataatt aggataagct taacaataaa
      421 agaactaata acgtttatta tctctcacgt agaacgatgg catcaaaaaa atcatctata
      481 aagaaagagt gaaagcgacg acagtttaat ttcactgcag gctgggtagc tccagcctgc
      541 tttcctgcat tacatcaccg cagcaaacgc ctttgccaca cgttgtacat ttgccgtatt
      601 taacccggcg acacacatgc gaccgctggc gatgagatag acaccaaatt cttcacgtag
      661 tcggtcaacc tgagcggcac ttaaaccggt ataactgaac atgccgcgct gattaagcag
      721 ataatcgaaa ttgcgttctg gcatctctgt gcttaatacc ttcaccagtt cctgacgcat
      781 tgccagaatg cgagtacgca tctcttctac ttccgccagc cagctggctt tcaatgcctc
      841 gtcattcagc actgcagcca ccacctgcgc accaaaattc ggcgggctgg agtagttgcg
      901 gcgaactgtt gctttcaatt gccccagtac gcggccagcg gcttcggcat cttcacacat
      961 aacagaaagt ccgccgacgc gctcgccgta aagggagaaa attttcgaga acgaattgct
     1021 caccagagcg ggtaatccag cgctggcaat ggcgcgaata gcgtaggcat cctcttccat
     1081 accggcacca aatccttgat aggcaatatc gaggaatgga ataagctcgc gggctttgag
     1141 aatttcaatc accgcatccc actggtcatt agtgagatcg gcacccgttg ggttgtggca
     1201 acatggatgc agcaacacaa tactgcgggc aggtaatgtt ttcagcgtcg ccaacaggtc
     1261 attaaagcgc acgccgttag tcgcttcgtc ataccagggg taagtactca cttcgaatcc
     1321 agccccggcg aatattgcta tgtggttttc ccaggtagga tcgctgaccc agacgcctga
     1381 ttccgggaag tagcgtttca ggaaatccgc gcccactttc aatgccccgg agccgccaag
     1441 ggtttgaatg gttgctacgc gctgttgttt cagtaccgga tggtccgcac caaacagcag
     1501 cggcgcaatg gcatggcgat agctgttaag cccttccatc ggtaaataaa gcgaagcgcc
     1561 atgaggctgc gcattcaggc gcgcttccgc ctccgccacg gctttcagtt gtggaataat
     1621 tccgtcttcg ttgtagtaca gaccgatact taaattcact ttgtcgctgc gagggtcttc
     1681 tttaaaacgc tccataagcg taagaatcgg gtcgccagcg taggcgtcaa ctttttgaaa
     1741 cacgcgatgg ttctccaggt ttacgggcag gtggttaaaa cacaataaac cggaagaagg
     1801 cgaagatcga gtggatgttc aggagcgaac ggcaattagc aacagagtga gactcatgac
     1861 aaacgtacat ccgccagagg cacgaccttt ataagagcat gaaagaacat caacttattg
     1921 aatttttagg attttattgg ccggataagg cattcacgcc gcatccggca cagacaatca
     1981 aatattacag aactattaat ccacgtattt catcgcgacc cttgaagtca ggcgcgtaat
     2041 aagttcgtaa gcgcttactt tcgtcatttc agcgatacgt tctacgggca aaccttcgcc
     2101 ccataaaatg accggatccc cggctttgtc ctgcgcctgt ggacctaagt ctacgcagat
     2161 catatccatc gccacgcgcc cgacaatcgg tacttcgcga ccgttcacca gcactggcgt
     2221 accggacggc gcggcgcgcg gataaccatc gccatagccc atcgcgacta cgccaagacg
     2281 ggtatcacgt tcgcttaccc aggttccacc ataaccaaca ggttctccgg ctttatgctc
     2341 acgcacggca atcaggctgg aggttagtga catcactggc tgacagccaa aatcggcacc
     2401 ggtggagcga tcttccagcg gcgagacgcc ataaagaatg atgcccgggc gcacccagtc
     2461 aaaatgcgac tgtggccaca gcagaatgcc acccgatgcg gcaatggaac gttgaccagg
     2521 tttgccttcg caaaaggtat taaagatagc gagttgtttc tcggttgcgc cacattttgg
     2581 ttcatccgcg cgcgcaaaat ggctgacgat attcaccggc tgacgaacgt ttttgcactg
     2641 ggtcaggcga tgataaaacg cctcagcctg ttccggcctt acgcccagac ggtgcatacc
     2701 ggtatcgagt ttcatccaga cggtaaccgg ctcgtccagg ctagcctctt ccagcgcagc
     2761 cagctgttct tcgttatgca cggcggtatg aaaatgttgc gcagaaatcg tcggcagatc
     2821 tctggcatca aaaaagcctt cgagtaacag tacaggtttg gtgattcccc ccgcacgcag
     2881 tcgcagagct tcttcgagac gggctacgcc aaaggcgtca gcatcgggga gcgttcgcgc
     2941 ggtctcaaga agaccgtgac cataagcgtt cgctttcacc accgcaacca ttttactggc
     3001 aggtgccagt tcacgaagac gttgcaggtt gtgtcgcaga gcgcggcggt taatcacaac
     3061 agttgccgct tgcatttgtg ttccttgata agtgtttgct ttaattacct aattcataaa
     3121 ataattatta ttcgtcgtcg tactgcggcc ccgcatagtt gtcgaagcgc gaccattgac
     3181 cgttaaaggt caggcgtacc gtcccgattg ggccgttacg ttgtttaccg ataataattt
     3241 ccgcgatgcc ttttaaatca ctgttttcgt gatacacctc atcacgatag ataaacatga
     3301 tcaagtccgc atcctgctcg atagagccag attcacgcag gtcggagttg accgggcgtt
     3361 tgtcggcacg ttgttccaga gaacggttca actgggacag cgccaccacc ggcacgttca
     3421 gttctttcgc cagtgctttc agcgagcgag agatttctgc aatttccagc gtacggttat
     3481 cggaaagcgc cggtacgcgc atcagttgca ggtagtcgat catgataagc ccgatgccgc
     3541 cgtgttcacg ggcaatacgg cgtgcgcggg aacgcacttc cgttggcgtc aggccggagg
     3601 agtcatcgat atagatattg cgtttttcga gcaaaatacc catggtgccg gaaatgcgcg
     3661 cccagtcttc atcatcgagc tgcccggtac ggattttagt ctggtcaacg cgcgacagcg
     3721 acgccagaga acgcatcata atctgttctg aaggcatctc cagcgagaag ataagtactg
     3781 gtttatcctg caacatcgcc gcgttttcga cgaggttcat cgcaaatgtt gttttaccca
     3841 tcgacggacg cgcggcgacg atgatcaaat ccgacggctg caagccagcg gtttttttgt
     3901 tgagatcgtc ataaccggtg tttactccgg taacgccatc gtgtggctgc tgaaacaact
     3961 gctcaatacg cgccacggtt gcgtcgagca catcggcgat gttcttcggc ccttcgtctt
     4021 tgtttgcacg actttcagca attttaaaga cgcgggattc agcaaggtcc agcagatctt
     4081 cgctggtgcg cccctgcgga tcaaaaccgg cttcagcaat ctcattcgca accgaaatca
     4141 tctcacggac aacggcacgt tcacgcacga tgtccgcata ggcactgatg ttcgccgcac
     4201 ttggcgtatt ttttgacagc tctgccagat aagcaaaacc accgacgcta tcgagttgcc
     4261 cctggcgttc cagcgattcc gcaagagtaa ttagatcgat agggctaccg ctttcctgca
     4321 aacgcgccat ttcagtaaag atatgacggt gtgggcgggt gtaaaaatcg tctgctacca
     4381 cacgctcggc tacatcatcc cagcgttcgt tatctagcat taaaccgccc aacaccgact
     4441 gctccgcttc gatcgagtgc ggaggcactt tcagcccggc aacttgtgga tcgcgttcgc
     4501 ggggttcagc ctgctgtctg ttgaagggtt tatttcctgc catagtgaat ggagttaccg
     4561 agataaagaa tgggtcgaaa ctttaccata tgaagcagac cctgacgata cgttctggag
     4621 gacacatggc aacacgaatt gaatttcaca agcacggtgg cccggaagta cttcaagccg
     4681 tagagttcac tcctgccgat ccggcagaga atgaaatcca ggtcgaaaat aaagccatcg
     4741 gcatcaattt tatcgacaca tatatccgca gcggccttta cccgccgcca tcgctaccca
     4801 gcggattagg caccgaagca gcaggcatcg tgagtaaagt cggcagtggt gtaaagcata
     4861 ttaaggcagg cgatcgtgta gtctatgcgc agtcggcgtt aggcgcttac agctctgtgc
     4921 ataacattat tgcggataaa gcggcgattc tgcctgcggc aatttctttt gagcaagctg
     4981 cggcatcctt cctgaaaggc ttaacggttt attatctgct gcgcaaaacc tatgaaatta
     5041 aacccgatga gcagttcctg ttccacgcag cagctggcgg cgttggcttg attgcctgcc
     5101 agtgggcaaa agccctgggc gcgaaactta tcggcaccgt aggaaccgcg caaaaagcgc
     5161 agagcgcgct aaaagcgggc gcgtggcagg ttattaacta tcgtgaagag aatctggtcg
     5221 agcggttaaa agagatcacc ggcggtaaga aagtgcgcgt ggtgtacgat tccgtgggca
     5281 gagacacctg ggaacggtcg ctggattacc tgcaacgccg cggcttaatg gtgagttttg
     5341 gcaactcatc aggtgcggtt accggtgtga acttaggcat tctcaatcag aaaggctcgt
     5401 tgtatgtgac acgcccttcc ctgcaaggct atatcaccac gcgggaggaa ttaaccgagg
     5461 ccagtaatga actgttctct ttgattgcca gcggtgtgat taaggtcgat gtcgccgagc
     5521 agcagaaata tccgctgaag gatgcgcagc gtgcgcatga aattctggaa agccgggcaa
     5581 cgcaaggttc cagcctgctg attccataaa agaaataggg cttccacctg ggaagccctt
     5641 tctttttata gttcggctgt atgtagggta cagcacgatg aatctgttag aggcgcaata
     5701 gtgacagatt tgattatcaa ttcctatttt gttctaagga taaaacctta ggttgtgatc
     5761 atccgcacaa tcccttagta acgccagcgg tcatcacgct gatatttcgg catttttggt
     5821 gctttaatcg ccttaataac ccacaccacc gcaatcgcca gtagtaacca cggcagcaac
     5881 ttaatcatca atgccagcat accgccgagg aacataatgg ccgtcgccac aaccagcgcg
     5941 gcgataatgc ccagcaacga aacgccggtg accatcagca tgacaaaaaa gccaatcaca
     6001 aaaagtagtt ccagcatgat gctctcccaa atatgaaatc tcttgctggc attacaagaa
     6061 tcatgccaaa aataatctat tgatttaaca gcaaaacgcc ccgcgacggt gcgcagggcg
     6121 tggtgaattt gactactttt tggtgaaaag ttaacgctta tccgccacca gtttgagcgc
     6181 gtgttccagc acgttaatgt cagcacccgc tttatgggca ttttcactta aataacgccg
     6241 ccactgccgc gcgccaggaa taccctggaa caaacccagc atatgccggg taatatggcc
     6301 gagatacgtc ccctggctga gttcacgctc aatgtacgga tacatggcgc gcactaccgc
     6361 caccggatcg gcatcggtat ccgaggaacc aaagatctcc cggtctaccg ccgccagaat
     6421 acccggattc tgatacgcct cgcgcccgac catcacgcca tccatatgtt gcaggtgtgc
     6481 tttggcctct tccagcgact tgataccacc gttaatcgac attgtcagat gcggaaagtc
     6541 acgcttcagt tgatacacac gcggataatc gagcggcggg atttcacggt tttctttcgg
     6601 gcttaacccc gaaagccagg ctttacgtgc gtggatgata aacatctcac actcgccttt
     6661 gccggaaaca gtgttgatga aatcgcagag aaattcatag ctgtcctggt catcgatgcc
     6721 aatacgcgtt ttcaccgtca ctggaatcga caccacatcg cgcatcgctt tcacacagtc
     6781 ggcaaccagc tgcgcattac ccatcagaca cgcaccaaac atgccgttct gcacccggtc
     6841 agacgggcag ccgacattca ggttgatctc atcatatcca cgcgcttctg ccagcttcgc
     6901 acactgtgcc agcgccgccg gatcgctacc gccgagttgc aacgctaccg gatgttcttc
     6961 ttcactgtac gccaggtaat cacctttacc gtgaataatc gcccctgtgg tcaccatttc
     7021 ggtatacagc aacgtattgc gggaaagcag acgcaagaaa tagcggcaat gtctgtccgt
     7081 ccagtcgagc attggtgcaa cgctaaaccg accactccag tgaacgtcag ttttttcagg
     7141 cattgcgctg gtttggttga ttttttgcat ttcagaatta ccgtgcattt tcaaatgtag
     7201 agattatttt atcgatatat cattgggtta tgttattcag catcactgtt caggaggctc
     7261 aatagcgggg tactatacca taacaacagg aagcgcctgt ctcattgcaa aagaaaattg
     7321 agatcaaatc aaggcatgaa gctctcacga agtgatggaa ataatcttat tagccgttag
     7381 ccttgttaag gacaatgata aacaatccag gttcgacgat aaataaaaaa tcacacatta
     7441 aactccggtg atatctcttc ctgctaatgg cactgataga agaaaaaaga acccaataag
     7501 tattaggttc ttttatgtaa tgccttccat accatcgaag aacttcacat attatttcgc
     7561 cgatttaacc ccgaataaat cataaattaa attagaagta tctgtaagta ttttaatctt
     7621 ttcctttgag tttgggtcaa acgacttagc aaagtcaatt aatttcggtg cagcatctcg
     7681 cattttgctt aaaatatcag gttcgagctt tccttcattc accagaagtg acattttatc
     7741 cagatagtca tcaaatttca tcccccccgg tccgccagat gcttcatctc tcccaggtac
     7801 ttcttcatat cacgttgaga taattcttca aactgagctg tcagatagtt ctcattatat
     7861 tcattaatat tcactttttc tgctccggct aaatctgtgt aggcactaga atatgaaagt
     7921 gaaattaaca gcgaagcaaa taattttaag ctgttgtcat catgttcctg gcaggcatta
     7981 acaaacgtta aaaaaccaga acctattgct tgaaaatgta tattcgctaa aggttcatta
     8041 tccttagatt cctcataaaa tagctgaacc gtggagggaa ggccattctt agtttctaca
     8101 tcaaaagtac atcgttcaat gggagtcgca tctttattat ccccaggatt acatatacta
     8161 ttaatcgcga aatgaaaaat agcccggtca acaactgatg caataaagtc acgatcgttt
     8221 aaatcctcat cagtgcaatc gtcaatatac ttattgaccc acattttgag atcatttatt
     8281 tgctcaatta aaaacacatt tttttacgtg catgccataa attatgcgtg attcgttgaa
     8341 taaacaaatc tatttttgca ataattatat cccgatgacg cagcgctgca taatcaa
//
LOCUS       DAZFVO020000194         8309 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFVO020000194 DAZFVO020000000
VERSION     DAZFVO020000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.195"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(6..212)
                     /locus_tag="ACHKNB_RS22140"
                     /old_locus_tag="pgaptmp_004428"
     CDS             complement(6..212)
                     /locus_tag="ACHKNB_RS22140"
                     /old_locus_tag="pgaptmp_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HGU4197816.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            525..614
                     /gene="kdpF"
                     /locus_tag="ACHKNB_RS22145"
                     /old_locus_tag="pgaptmp_004429"
     CDS             525..614
                     /gene="kdpF"
                     /locus_tag="ACHKNB_RS22145"
                     /old_locus_tag="pgaptmp_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HGU4197817.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            614..2287
                     /gene="kdpA"
                     /locus_tag="ACHKNB_RS22150"
                     /old_locus_tag="pgaptmp_004430"
     CDS             614..2287
                     /gene="kdpA"
                     /locus_tag="ACHKNB_RS22150"
                     /old_locus_tag="pgaptmp_004430"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HGU4197818.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHKNB_RS22155"
                     /old_locus_tag="pgaptmp_004431"
     CDS             2310..4358
                     /gene="kdpB"
                     /locus_tag="ACHKNB_RS22155"
                     /old_locus_tag="pgaptmp_004431"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HGU4197819.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHKNB_RS22160"
                     /old_locus_tag="pgaptmp_004432"
     CDS             4367..4939
                     /gene="kdpC"
                     /locus_tag="ACHKNB_RS22160"
                     /old_locus_tag="pgaptmp_004432"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HGU4197820.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHKNB_RS22165"
                     /old_locus_tag="pgaptmp_004433"
     CDS             4932..7616
                     /gene="kdpD"
                     /locus_tag="ACHKNB_RS22165"
                     /old_locus_tag="pgaptmp_004433"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HGU4197821.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQELTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHKNB_RS22170"
                     /old_locus_tag="pgaptmp_004434"
     CDS             7613..8290
                     /gene="kdpE"
                     /locus_tag="ACHKNB_RS22170"
                     /old_locus_tag="pgaptmp_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HGU4197822.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1717 a         2161 c         2439 g         1992 t
ORIGIN      
        1 agcaatcaat aaaaatcacc agttgccttt tcggcctgat ccatccacac cggtttatcg
       61 ctggttttcg accagacgcg atgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      121 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat aagtacggcg taaaaagata
      181 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      241 gtgaatggca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      301 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcatcc
      361 tgtaattaag ttacacaaaa gttaaattaa tactaaacat tagttaaatc atggcttttg
      421 ccatttttat acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga
      481 attctaccct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      541 taaccggcgt attgctggtg tttttattac tgggttatct ggtttatgcc ctgatcaatg
      601 cggaggcgtt ctgatggctg cgcaagggtt cttactgatc gccacgtttt tactggtgtt
      661 aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct
      721 tcccggtaca acgggcgttg agcgcgtact ttttcgcgca cttggcgtct ctgaccgtga
      781 gatgaactgg aagcaatatc tctgtgccat tctcggcctg aacatgctgg ggctggcggt
      841 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      901 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      961 atcttacagc ggtgaaacca cgttgagcta tttcagccag atggctggct taacggtgca
     1021 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccctcatcc gtgcgtttac
     1081 ccgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
     1141 ggtgctcgtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag gtgcgctgca
     1201 aaactttcag ccttatcagg ctgtgaatac cgttgaagga gcgcaacagc tgttacccat
     1261 ggggcctgta gcttctcagg aagcgatcaa gatgctcggt actaacggcg gtggcttctt
     1321 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
     1381 gctggcgatc ttcttgatcc caacggcgct gtgctttgcc tttggtgaag tgacgggcga
     1441 tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1501 cgtggtgatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1561 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1621 ggtcgtgact acggcggctt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1681 tctcggtggc atggtgccga tgtggctgat gcaaattggt gaagtggtgt tcggcggtgt
     1741 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1801 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact
     1861 gactgcactg gcgattctgg ttaccccgac gctggtgctg atgggcgcgg cgctggcaat
     1921 gatgaccgac gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt
     1981 gctgtacgcc gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     2041 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     2101 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     2161 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     2221 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccagtgg cggaatatct
     2281 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     2341 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     2401 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2461 tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2521 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2581 aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg
     2641 agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca
     2701 tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2761 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2821 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2881 gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2941 agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct ctgaccatcg
     3001 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgccgtga
     3061 gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     3121 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg attgccacca
     3181 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat aaaaccggca
     3241 ccatcacact cggtaaccgt caggcgtcgg agtttatccc tgcacagggc gtggatgaaa
     3301 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggccgca
     3361 gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3421 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc
     3481 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggtggtc
     3541 acttccctac cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg gccacgccgc
     3601 tggtggtggt ggaaggttcc cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3661 gcggtattaa agagcgcttc gcccagctgc gcaaaatggg cattaaaacg gtgatgatta
     3721 ccggcgataa ccgtctgact gccgccgcca ttgctgcgga agcgggcgtc gatgattttc
     3781 tcgccgaagc gacaccggag gccaagctgg cattgattcg tcagtatcag gcggaaggcc
     3841 gtttggtggc gatgaccggc gacggcacca acgatgctcc agcgctggcg caggcagatg
     3901 tcgcggtggc gatgaactcc ggtactcagg cggcgaaaga agcgggcaat atggtcgatc
     3961 tcgactctaa cccgaccaag ctgatcgagg tggtacatat tggcaaacag atgctgatga
     4021 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     4081 ttccggcggc gttcgcggca acgtatccgc agttaaatgc gctgaacatc atgcgccttc
     4141 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     4201 tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     4261 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     4321 tcattgattt actgctgacc gtttgcggtc tggtgtgagg tttaccatgc gtggcttacg
     4381 tccggcatta tcgacattta tctttctgtt attgattact ggcggcgttt acccgctgct
     4441 gaccaccgtg ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgattcgtga
     4501 aggtgatacg gtgcggggtt cggcattaat cgggcagaat tttaccggca acggctattt
     4561 tcagggtcgc ccgtcggcaa cggcagaaat gccctataat ccacaggctt ctggcgggag
     4621 caatctggcg gtcagtaacc ctgagctgga taaacaaata gccgcacgcg ttgctgcatt
     4681 acgggccgct aacccggatg ccagtacgaa cgttccggtt gaactggtga cggcatcggc
     4741 aagcgggctg gacaataata tcaccccgca agcggcggtc tggcaaatcc cacgcgtggc
     4801 gaaagcgcgt aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca
     4861 accgctggtg aaatatatcg gccagccggt cgtcaacatt gttgaactca atctggcgct
     4921 ggataaactt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4981 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga
     5041 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     5101 ttggcgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa gggctggctg
     5161 ttctgccgcc caaacgccag gcgtaccgtg ggcggcatat cagcgagttt gatctcgatg
     5221 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     5281 caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa gctggcattg
     5341 atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     5401 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5461 tagtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5521 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5581 ccctgcgtga actggcactg cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct
     5641 ggcgggggca tcctggtgaa gagaaagtgt ggcacacgcg cgacgcgatc cttttatgca
     5701 tcgggcataa caccggcagt gagaaactgg tccgcgcggc ggcgcggttg gcctcgcgcc
     5761 tgggtagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaaa
     5821 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5881 ctacactttc tgatccagcg gaagaaaaag cagtagtgcg ttatgcccgt gaacataatc
     5941 tcggcaagat tattctcggt cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg
     6001 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     6061 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     6121 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcact ttaattgcca
     6181 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtgg
     6241 tggtggtggc gctattttat ggacgctggc cttcggtggt tgctaccgtc attaatgtgg
     6301 tgagtttcga tctctttttt atcgccccac gcggcacgct cgctgtctct gatgtgcaat
     6361 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgcgg
     6421 gtgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6481 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6541 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6601 aattgcagcc gttaacacat ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt
     6661 ggagttttga taaaggcctg cctgcgggtg cgggcaccga cacgttaccc ggcgtgccgt
     6721 accagatttt gccgctaaaa agcggcgaga aaacctacgg actggtggtg gtggaaccgg
     6781 ggaatctgcg tcagttgatg atcccggagc agcagcgcct gctagagacg tttacgctgt
     6841 tagtcgccaa tgcgtttgag cggctgacgc tgaccgccag tgaagaacag gcgcggatgg
     6901 caagcgaacg tgaacagatc cgcaacgccc tgctggcggc gctttcgcat gatttacgca
     6961 cgccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     7021 gatcgcccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     7081 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     7141 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     7201 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     7261 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     7321 ccgaaattgg tatcgatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata
     7381 acggccccgg tcttccgcca ggccaggagc tgacgatatt tgataagttt gctcgcggga
     7441 ataaagagtc ggcagtaccg ggggtagggc ttggactggc aatttgtcgg gcgatagtgg
     7501 atgtacacgg gggcactatt accgcgttca accgaccgga aggtggtgcc tgttttcgtg
     7561 ttacacttcc ccagcaaacc gctcctgaac ttgaagactt tcatgaggat atgtgacaaa
     7621 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7681 cgacgggatg cgcgtctatg aggccgaaac gctgcaacgc ggcttgctgg aagcggctac
     7741 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7801 tatccgcgac ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga
     7861 agagagcgac aaaatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt
     7921 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7981 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     8041 tcaccggggt gaggaagagg tgcatctcac gccgattgag ttccgcctgc tggcggtgct
     8101 gctgaataat gccgggaaag tgctcaccca gcgccagtta ctcaatcagg tgtgggggcc
     8161 aaacgcggtc gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact
     8221 ggaacaggat cccgctcgcc cccgccattt cattactgaa accggtattg ggtatcggtt
     8281 tatgccatga atgtttattc aataaatgc
//
LOCUS       DAZFVO020000195         8228 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFVO020000195 DAZFVO020000000
VERSION     DAZFVO020000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.196"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            353..880
                     /locus_tag="ACHKNB_RS22175"
                     /old_locus_tag="pgaptmp_004435"
     CDS             353..880
                     /locus_tag="ACHKNB_RS22175"
                     /old_locus_tag="pgaptmp_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGU4197823.1"
                     /translation="MSARGINKVIVVGRLGKDPEVCYIPNGGAVANLQVATSETWRDK
                     QTGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            938..1171
                     /locus_tag="ACHKNB_RS22180"
                     /old_locus_tag="pgaptmp_004436"
     CDS             938..1171
                     /locus_tag="ACHKNB_RS22180"
                     /old_locus_tag="pgaptmp_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4197824.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEGGAGYWMNHVIRDFGILK"
     gene            1241..3199
                     /locus_tag="ACHKNB_RS22185"
                     /old_locus_tag="pgaptmp_004437"
     CDS             1241..3199
                     /locus_tag="ACHKNB_RS22185"
                     /old_locus_tag="pgaptmp_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGU4197825.1"
                     /translation="MSVAEFKAKTGRKSSRKPSKEQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAGSIQGVGLLQNLVVHTLPGERYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLAAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQDVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQMDATEHTTNLADAA"
     gene            3254..3688
                     /gene="psiB"
                     /locus_tag="ACHKNB_RS22190"
                     /old_locus_tag="pgaptmp_004438"
     CDS             3254..3688
                     /gene="psiB"
                     /locus_tag="ACHKNB_RS22190"
                     /old_locus_tag="pgaptmp_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HGU4197826.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3685..4404
                     /locus_tag="ACHKNB_RS22195"
                     /old_locus_tag="pgaptmp_004439"
     CDS             3685..4404
                     /locus_tag="ACHKNB_RS22195"
                     /old_locus_tag="pgaptmp_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HGU4197827.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            4401..4997
                     /locus_tag="ACHKNB_RS22200"
                     /old_locus_tag="pgaptmp_004440"
     CDS             4401..4997
                     /locus_tag="ACHKNB_RS22200"
                     /old_locus_tag="pgaptmp_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197828.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            5462..5962
                     /locus_tag="ACHKNB_RS22205"
                     /old_locus_tag="pgaptmp_004441"
     CDS             5462..5962
                     /locus_tag="ACHKNB_RS22205"
                     /old_locus_tag="pgaptmp_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HGU4197829.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(6274..6342)
                     /locus_tag="ACHKNB_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /pseudo
     CDS             complement(6274..6342)
                     /locus_tag="ACHKNB_RS22210"
                     /old_locus_tag="pgaptmp_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            6694..7128
                     /locus_tag="ACHKNB_RS22215"
                     /old_locus_tag="pgaptmp_004443"
     CDS             6694..7128
                     /locus_tag="ACHKNB_RS22215"
                     /old_locus_tag="pgaptmp_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HGU4197830.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            7221..7487
                     /locus_tag="ACHKNB_RS22220"
                     /old_locus_tag="pgaptmp_004444"
     CDS             7221..7487
                     /locus_tag="ACHKNB_RS22220"
                     /old_locus_tag="pgaptmp_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197831.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            7552..>8228
                     /locus_tag="ACHKNB_RS22225"
                     /old_locus_tag="pgaptmp_004445"
     CDS             7552..>8228
                     /locus_tag="ACHKNB_RS22225"
                     /old_locus_tag="pgaptmp_004445"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HGU4197832.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEDPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSP"
BASE COUNT         1732 a         2266 c         2522 g         1708 t
ORIGIN      
        1 aggaaactgc gcctgatggc gcagcagtcc tccctcctgc cgccacctct gcggcctgtt
       61 tcccgctgtg gactttccgg ggggcagacg taaccccctc ccgttccatc tgtccagggt
      121 gagatgcacc ggccacaaaa tttttagcca tgaacccggc aaaaaatttt gcgtccgccc
      181 ccggacagct gtcccgtgag gcggtaaacg ggcccccatc ggaaaggtcc actgcgggga
      241 gaaaccccag tgaagtggac ggcaggagga gctttcgctc ctctgaaggg gcacatgaac
      301 gtcccgtcgc cggtatccgg tttatctcac atctttcagt caggaggttc ttatgagtgc
      361 acgtggtatc aacaaggtca tcgtggttgg tcgtctgggt aaagatccgg aagtctgtta
      421 catccccaac gggggcgcag tggcaaacct gcaggtggcc acgtcagaaa cctggcgcga
      481 caaacagacg ggggagatac gggagcagac ggaatggcac cgcgtggtgc tgttcggcaa
      541 gctcgcggaa gtggcaggtg aatatctgcg caagggcgcg caggtctaca tcgaaggcca
      601 gcttcgcacc cgtagctggg aggataacgg tatcacccgt tacgtcaccg aaatccttgt
      661 taaaaccacg ggcaccatgc agatgctggg gagtgcacca cagcagaacg ctcaggcgca
      721 accgcagcct cagcagaatg ggcagccaca gagtgctgac gcgacgaaaa aaggtggcgc
      781 gaaaacgaaa ggccgtggac gtaaggccgc gcagcccgag cctcagccgc aaccgccgga
      841 gggggaggat tacgggtttt cagacgatat cccgttctga ctggcctgaa tgtgataacc
      901 gccccgccct gcgcggggca tcaccggaga gatgaggatg agcgaatatt tcagaatact
      961 tcagggactg cctgacggtc cttttacccg caaacaggcc gaagccgttg ccgcacagta
     1021 ccggaacgtc tttatcgagg atgaccacgg cgaacagttt cgcctggttg tccgtaataa
     1081 cggtgcaatg gtctggcgca cctggaattt tgagggcggt gccgggtact ggatgaacca
     1141 tgtcatccgt gatttcggga ttcttaagta agagaggtgc cggacgggaa cgcgtccggc
     1201 agacataacg aaggttaacc acctgtaaag gagcatcttt atgtcagttg cagagtttaa
     1261 ggcaaaaact ggtcgtaaat ccagccgtaa accatcaaaa gaacaggaaa ccgccctgtc
     1321 tgccctgctg gcgcagacgg aggaagtgag tgtgccgctg gcttcactga ttaagtcacc
     1381 gctgaatgtg cgcactgtgc cgtattctgc ggagtccgtc agcgaactgg cagggtccat
     1441 tcagggcgtc ggactgttgc agaatctggt cgtgcatacc ctgcctggtg agcgctacgg
     1501 tgtcgccgca ggtggtcgca gactggcagc actcaacatg ctggcagagc gtggaatcat
     1561 tccggctgac tggcctgtcc gcgtgaaaat tattccgcag gagctggcga ctgccgcatc
     1621 aatgactgaa aacggtcatc gtcgggatat gcaccctgcc gaacagattg ccggattccg
     1681 tgcaatggcg caggaaggca aaacacctgc acaaatcggt gatttgctgg gctattcacc
     1741 ccgccacgtt cagcgaatgc tgaaactggc agaccttgcg cctgtcatcc tcgatgcgct
     1801 ggcagaagac cgcatcacca ccgaacactg tcaggcgctg gcgctggaga acgacaccgc
     1861 gcgtcaggtg caggtgtttg aagccgcctg ccagtcagga tggggcggta aaccggatgt
     1921 gcgggttatc cgcaacctga ttaccgaaag tgaagtggcg gtggcgggga acagtaaatt
     1981 ccgcttcgtg ggggctgatg ccttctcgcc agacgaactg cgcaccgatt tgttcagcga
     2041 tgacgagggg ggctatgtcg accgcgtggc gctcgatgcc gccctgctgg aaaaactcca
     2101 ggctgttgct gaacaccttc gggaagccga aggctgggaa tggtgcgccg ggcgcatgga
     2161 gccagtcggt gagtgccgtg aggatgccgg aacataccgc tgtctgccgg agccggaagc
     2221 ggtgctgacg gaggcggaag aagaacgcct gaacgaactg atggcacgtt acgacgcact
     2281 ggaaaaccag tgtgaggaat ccgacctgct ggcagcagaa atgaagctga tgcgctgcat
     2341 ggcgaaggtc agggcgtgga cgccggagat gcgtgccgga agcggtgtgg tggtgtcctg
     2401 gcgttatggc aacgtgtgtg tccagcgtgg tgtgcagttg cgcagcgagg atgacgcggc
     2461 tgccgacgct gaccgcacgg aacaggtgca ggagaaagcg tcagtggagg aaatcagtct
     2521 gccactgctg acgaaaatgt cttccgaacg cacgctggca gtccaggcgg cactcatgca
     2581 gcaaccggac aaatccctga cactgctggc atggacgctc tgcctgaatg tgtttggcag
     2641 cggggcgtac agtaaaccag cacaaatcag cctggaatgt gaacattatt cgctgaccag
     2701 cgatgcgcca tcggggaagg aaggtgccgc attcatggcg atgatggcag aaaaagcccg
     2761 tcttgcagcc ctgttaccgg agggatggtc acgggatatg acgacgttcc tgtcactcag
     2821 ccaggacgtg ctgttatcac tgctcagttt ctgcaccgcg tgcagcatcc acggcgttca
     2881 gacccgcgag tgtggtcaca cgtcacgcag tccgctggac acgctggaga gcgctatcgg
     2941 attccacatg cgcgactggt ggcagccgac aaaagcaaac tttttcggac acctgaaaaa
     3001 gccgcagatt atcgatgccc tgaatgatgc cggactgtcc ggtgctgcgc gggacgcgga
     3061 gaaaatgaag aaaggcgatg cggctgaaca tgcagagttc catatgaaag acaaccgctg
     3121 ggtgcctggc tggatgtgta caccacgtcc acagatggat gccactgaac acaccactaa
     3181 cctggctgat gccgcctgat gaacaaccac accgccccgc cggagacggg gcggcagcaa
     3241 gggagatacc gtgatgaaaa ctgaactgac cctgaatgcc ctgcagtcca tgaacgcaca
     3301 ggaatatgaa gatatccgtg ctgcgggaag cgatatgcgc cgtaatctca ctcacgaggt
     3361 gatgcgtgaa gtggacgcac cggctaactg gatgatgaat ggcgagtatg gcagtgagtt
     3421 cgggggcttt ttccccgtcc aggttcgttt cacgccagcc cacgaacgtt tccacctggc
     3481 attatgttcg ccgggagacg tctctcaact ctggatgctg gttctggtga atggtggtgg
     3541 acagcctttc gccgtcgttc aggtgcaaca tatcttcacg cctgtcgcga tcagtcacac
     3601 gctggcgctc gccgcgacac ttgatgcgca gggatacagt gttaacgaca tcattcatat
     3661 tttgatggca gaaggaggtc aggcatgagc gcacgttcac gggcactgat ccccctcagc
     3721 gcagaacaac aggccgccat gcaggctgtg gctgtcactg aacaacgtcg tcgtcaggga
     3781 cgcacacttt cagcatggcc ttatgccagc gctttctttc gctgcctgaa tggcagtcgc
     3841 cggatttcgc tgaccgatct ccgctttttt gcccctgcgc tgacgaagga ggaatttcat
     3901 ggcaaccgtc tcctgtggct ggctgccgtg gataaactga ttgaaagttt tggtgaagtc
     3961 tgtgttcttc ccctgccatc cgatgcgggg catcgtctgt tcccgtccgt tccttttcgt
     4021 gaaggtgagc gtcgtcgtca gaaaaccacg ctgacagagc agaaatacag ccgccagcgg
     4081 gaacgtgagg cagaacgacg ggaactggaa taccagacat gttttgctca ggcgcagatt
     4141 gaccttgcgt ttcatactcc cgccacggtc ggaagctggt tgtcccgctg gtctggtgtt
     4201 gttgaggagc atgatctgga aacgattttc tgggggtggt gcgggcgttt tccatcactg
     4261 tcatcatttg accggttttt ctggcaggag gaaccactct ggcggctgat ttttgaagcc
     4321 ggtgaggccg gtcgtggtgc accggtacag gtacgtgcac ttgagcagtg gatgatcccg
     4381 aacaagctgg agaacgcaat atgatgaaat cagacgaaca atatcaggtt cccgtgtgga
     4441 tgcgacctct gttgccgttg ctctgcaata ccggtggtaa tgatccggag gaactgctga
     4501 atgatacaga aaccaccgcc agtgcgaata ttgtccgtta tgtactgata gttgcagtgc
     4561 ggtcgcagat tgatctgctg cagctactgt acaggaaagg actgttgcgc acagagatac
     4621 caggtggctt ttcaccggaa gaagcgcagg aactcctgga taatctggtg cgcagccata
     4681 tcagcaaggc gctgtcgggt gagcgaatgg cagcccgtga cagaaatgcc gatctggcct
     4741 ggattcgcca gcaactggtc gatgctgcct ggtttgtccg tgccacactg gaagcgcatg
     4801 ggatgggcgt cggaaatgag agtccctctg ctccgccgga gacaatgccg gacatacaga
     4861 cacgggaact ggtgatgttg atcaagcgac tggcatcttc tctgaaagcg gtgaaaccgg
     4921 acagttgtgt ggtgcgtgaa gcgcaggact ggctgcgcga cagaaaactt gtgagcatca
     4981 cggatgttct tcggtgaacc agtggaggta tgcttgaggg gcagggacat gccggtcatg
     5041 atcgtttcat atcaggggtg gtctggaggt catgcggcgt gtcctctgct ctcaccggaa
     5101 taaggacgtc gccggcggct ccgcttttac ccggccatgc ggggcatggc cttgtgggtt
     5161 ttcagttatg tggcctcagc gtcgtgtgcg ggctgtgccg tgcctccatc gtagccgggc
     5221 ttctgcgggc gggatgcaag gtaagatgaa ccgctgcggt cacccggtcc ctccttcccg
     5281 tctgccgtga ttttccggtc gttcacccgc tcagatttcg cggtctcacc ctccaactcc
     5341 cgtcagccgt gcgcgtcggg ctgcggcttc ccttgcgccc ttgcatccct gcaggcccgg
     5401 caaagatgga ggcacggcac cgcccggtgt cgcagaacat gtgactatgg aggattcagg
     5461 aatgtctgtt gttgcacctg ctgtatacgt tggaacctgg cacaaatata actgcggaag
     5521 catcgccggc cgctggtttg acctgaccac gtttgatgat gaacgcgatt ttttcgccgc
     5581 ctgccgtgcc cttcaccagg atgaaaccga tccggaactg atgtttcagg attatgaggg
     5641 tttcccgggg aatatggcct ctgaatgtca tatcaactgg gcctgggttg aaggcttccg
     5701 ccgggcacgg gatgaaggct gcgaagaggc ttatcgtctc tgggtggatg ataccggtga
     5761 gacggatttt gacagcttcc gcgatgcctg gtggggcgag gctgacagtg aggaggcttt
     5821 tgcggttgag ttcgtcagtg ataccggcct gctggctgac gtgccggaga cggtggcgct
     5881 ctattttgac tatgaggcgt atgcgcggga tttattcctg gactccttca cctttattga
     5941 cggtcatgtg ttccgtcggt gatttactcc cccgctccgg cgggggtatg gcctgccggg
     6001 ccgccggaaa accctggcag gcttacgcct gcctgaaata caggagggcc gcgcctgcgg
     6061 cgctgccctt cctgcccgtc atggtgtgag gcatttttct gttttgtcgt ttctgttgtt
     6121 ctgactcccg ggggcgggga caccttcggc tcccggctgc ctggcggcat ccggtcgtca
     6181 ggcccgactc cgacaggcag ctgctggcgc acccgccagt ctccgccgaa cccgctggcg
     6241 cggtttcggg ctgccgctac ggtccgatgc gtgaaaaccc gggcttgcag cgaccgttcc
     6301 ggtcgcgccg cctctgtcgc gccgtgacct gcgctccctg cacacctgac ggtgtgtaag
     6361 gaaactgcgc ctgatggcgc agttgtctgc ccttccgccg ccacccctgc ggcctgtttc
     6421 ccgctgtgga ctttccgggg gctgaagcgg ccacaacacg tgtcagccgt ccagggggga
     6481 gatgtaccag ccacaaaact ttttgccgca agcgtcaaaa acttttgcgt ctgcccccgg
     6541 acaactgccc cgtgccgtgg ctgtcgctgc cccatcggaa aggtccactg cggggagaaa
     6601 ccccgaagaa gtggacggca gaaggggctt tcgcccctcc gggggagcaa ccggtcggcc
     6661 cggcgacctg attcagcaga aggagacgtt ttcatgacag tcagcagcac gatttccgtt
     6721 ttttgccgcg acggggtgtt ccgtaccgtt tactgccacc tgcacggtga gccgacctgg
     6781 aacggtcgca tcctgcatac ccactatgcc accggtcagc aggccgaagc cctggttgaa
     6841 cacggtgata tccgttgcct cggtccccgt tgcgacaaac ccgccgggca tacgcttcag
     6901 aacccggtgg acggtgtgac ggcttattac ggacgtgaca gtggtttccg gatggacagt
     6961 gaggcgcgtg agtaccgttc tttcatggag gctattgcca cggaaagcac agaagaggtg
     7021 cgcttccatt atgtgttcat cgacggctac tggaaggtga tgtaccgcac gccggaaggc
     7081 tggaagatga aagcgctcgc gctggcactg cgtcgctgtc cggaatgaaa aaaagcaggg
     7141 cgataaaccc tgcctttctc cgccggcgct tccccgtacg gaagtccggc atctcattca
     7201 ctaactatgg agattatgcc atgagaccat caattatctt cgcaaccgcc gagtatgtaa
     7261 agcgtctgcg tgaagagtgc ctgcgggaga acaaacccct gcaccgccat acccgcttca
     7321 gacgccagga actggcaccg gatgagatta acccggacgt cctggcgatg ggcggtcata
     7381 tcgccagacg ctgcagtgag cagaagcggg tacgtatccc cgccatgaaa gtcagcgaat
     7441 ggggccacct gctccgcgcg cttgaaattg agcgggtctg ccactgatta acccttgccg
     7501 tctgcctgtg caggcggcag gatacgttca gacatctcac caggaggaac cgtgagtaag
     7561 aagaaaaaca ccacaacgcc cacgccgcat gatgccgcgt tccggtcgtt cctggcgaat
     7621 cccgacgtcg ccagagattt tctggaactg catcttccgg cggagtaccg gcagttgtgc
     7681 gacctgtcca cgctgaagct ggaacccgcc acctttgttg agccggacct gcatcagtac
     7741 gccagcgata tcctctggag tgtgaaaacc accggggggg aagacggcta tgtttatacg
     7801 ctcatcgagc accagagcac cgaaaatctg tacatgcctt tccgcatgtt acgttacagt
     7861 gtggcggcga tgcagagaca tctggagcag cacaaaacgt tgccactggt cattccggtg
     7921 ctgttctatc acggtgagcg cagcccgtac ccgtacagca tgaactggct ggactgtttt
     7981 gaggatccgg cacttgcggc taaaatatac acaaagccgt ttccgctggt tgatatcact
     8041 gtcgttgatg acaatgaaat catgaaccat cgccggatgg ccgcactgac gctgctgatg
     8101 aagcatatcc gccatcgtga catgatggag ctgctggaca aactcccgca ggtcatggtg
     8161 gaaatttcag acgagcaggt gcgtgttctg attcattaca tcgttaacgc aggggactct
     8221 gtatcacc
//
LOCUS       DAZFVO020000196         8065 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFVO020000196 DAZFVO020000000
VERSION     DAZFVO020000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 8065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.197"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKNB_RS22230"
                     /old_locus_tag="pgaptmp_004446"
     CDS             complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKNB_RS22230"
                     /old_locus_tag="pgaptmp_004446"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HGU4197833.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKNB_RS22235"
                     /old_locus_tag="pgaptmp_004447"
     CDS             complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKNB_RS22235"
                     /old_locus_tag="pgaptmp_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HGU4197834.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEGSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKNB_RS22240"
                     /old_locus_tag="pgaptmp_004448"
     CDS             3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKNB_RS22240"
                     /old_locus_tag="pgaptmp_004448"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HGU4197835.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4937..5551
                     /locus_tag="ACHKNB_RS22245"
                     /old_locus_tag="pgaptmp_004449"
     CDS             4937..5551
                     /locus_tag="ACHKNB_RS22245"
                     /old_locus_tag="pgaptmp_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HGU4197836.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKNB_RS22250"
                     /old_locus_tag="pgaptmp_004450"
     CDS             5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKNB_RS22250"
                     /old_locus_tag="pgaptmp_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HGU4197837.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKNB_RS22255"
                     /old_locus_tag="pgaptmp_004451"
     CDS             7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKNB_RS22255"
                     /old_locus_tag="pgaptmp_004451"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HGU4197838.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            7974..>8065
                     /locus_tag="ACHKNB_RS22260"
                     /old_locus_tag="pgaptmp_004452"
     rRNA            7974..>8065
                     /locus_tag="ACHKNB_RS22260"
                     /old_locus_tag="pgaptmp_004452"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1817 a         2159 c         2185 g         1904 t
ORIGIN      
        1 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
       61 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
      121 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
      181 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
      241 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
      301 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
      361 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
      421 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
      481 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
      541 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
      601 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
      661 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
      721 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
      781 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtggctc
      841 attggcacat ggcccatatg ctccacgccg ccgacaagac atgcctgcgc atcgccagtc
      901 atgatcattc gtgctgcgtc atgcaatgcc tgcatggatg aaccacacaa gcgattgacg
      961 gtcaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatatta
     1021 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
     1081 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
     1141 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
     1201 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
     1261 ggctcaaccg ggggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
     1321 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
     1381 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
     1441 accagcgcca tatccgcttc tgccggagtg gcgataatgc tttcttccag acagcgcacc
     1501 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
     1561 ttcggctggc tcacttctgc cagcaggtcg tcaacgacgg cgtcttcttc tttcttcggc
     1621 ttacctttgc tgccttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
     1681 ttggcatcaa acagcgcgtc gatggtatcg cgataatctt tctgcatccg ctgcgggaag
     1741 cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa tgcccacgac atccagcaga
     1801 tatgccggac ccatcggcca gccaaactgt ttttccatta ctttgtcgat cttgcggaaa
     1861 tccgcgccgt cgcgcagcaa caggctgaaa ccggcgaaat acgggaacag cacgcggttg
     1921 acgaagaagc cggggcagtc gttaaccaca atcggcgttt tgcccatctt gctcgcccag
     1981 gcgacgactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaattat ttctaccaac
     2041 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttctgg acgttccagc
     2101 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtattcggg
     2161 cgtactttct gctcggtttc tgccagtacg gcttttttca cttttgggtt ttcgacaacc
     2221 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
     2281 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
     2341 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
     2401 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
     2461 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
     2521 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
     2581 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
     2641 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
     2701 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
     2761 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
     2821 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
     2881 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
     2941 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
     3001 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
     3061 tcgcagccac cgcccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
     3121 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
     3181 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
     3241 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
     3301 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
     3361 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
     3421 gtctcctgaa tccgcaaggt gatctggtcg taccagatgc atcgaagtgt gtattttgtg
     3481 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
     3541 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
     3601 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
     3661 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
     3721 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
     3781 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
     3841 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
     3901 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
     3961 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
     4021 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
     4081 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
     4141 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
     4201 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
     4261 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
     4321 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
     4381 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
     4441 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
     4501 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
     4561 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
     4621 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
     4681 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
     4741 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
     4801 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
     4861 gcattcgtat cgaagacaac gtggtgatcc acgaaaataa cgtggaaaac atgacccggg
     4921 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
     4981 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
     5041 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
     5101 gtgggtggcg ggtgcgccgg atgattcaca acagctgggt ttctctgacg acggggagcc
     5161 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg ttggggaaat
     5221 taccgctgtg gtggtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
     5281 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
     5341 attaaccgaa tatactttgc aatgtgaata tcatcagtta accggcattg aagcgttgct
     5401 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
     5461 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
     5521 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
     5581 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
     5641 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
     5701 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
     5761 cgcaaagaga aaggcgaact taaatcccgt gaggggtttc tgatagtggt gctgttctgg
     5821 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
     5881 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
     5941 gtggggctgg attcgctccc tcacgccatc ctcttttatc gccagatgct gcaatggttt
     6001 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
     6061 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
     6121 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
     6181 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
     6241 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga tagcccgact
     6301 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
     6361 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
     6421 atcggcgtac agtttacgct ggtggttatt tgtaccctcg tactgtggtt tcataatgtc
     6481 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtgtc gatggcgaca
     6541 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
     6601 ttatgttcgg cttttatcgg cggttgtgcc gggtcaacgg gcggtggcct gaaagtgatc
     6661 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
     6721 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
     6781 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
     6841 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
     6901 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
     6961 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
     7021 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
     7081 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
     7141 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
     7201 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
     7261 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
     7321 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
     7381 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
     7441 gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca ggcggtgaaa
     7501 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
     7561 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc acaaagaata aaaaatgcgc
     7621 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     7681 accactgaca cggaacaacg gcaaacacgt tgccgggtca gcggggttct cctgagaatc
     7741 tcaacagaga aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc
     7801 cgcgccgctg agaaaaagcg aagcggcact gctctttaac aatttatcag acaatctgtg
     7861 tgggcactcg aagatacgga ttcttaacgt cacaagacga aaaatgaata ccaagtctca
     7921 agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
     7981 agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac
     8041 ggtaacagga agcagcttgc tgctt
//
LOCUS       DAZFVO020000197         7896 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFVO020000197 DAZFVO020000000
VERSION     DAZFVO020000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.198"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..1071
                     /locus_tag="ACHKNB_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /pseudo
     CDS             1..1071
                     /locus_tag="ACHKNB_RS22265"
                     /old_locus_tag="pgaptmp_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1040..2776)
                     /gene="aceK"
                     /locus_tag="ACHKNB_RS22270"
                     /old_locus_tag="pgaptmp_004454"
     CDS             complement(1040..2776)
                     /gene="aceK"
                     /locus_tag="ACHKNB_RS22270"
                     /old_locus_tag="pgaptmp_004454"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HGU4197839.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3057..4361)
                     /gene="aceA"
                     /locus_tag="ACHKNB_RS22275"
                     /old_locus_tag="pgaptmp_004455"
     CDS             complement(3057..4361)
                     /gene="aceA"
                     /locus_tag="ACHKNB_RS22275"
                     /old_locus_tag="pgaptmp_004455"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HGU4197840.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4391..5992)
                     /gene="aceB"
                     /locus_tag="ACHKNB_RS22280"
                     /old_locus_tag="pgaptmp_004456"
     CDS             complement(4391..5992)
                     /gene="aceB"
                     /locus_tag="ACHKNB_RS22280"
                     /old_locus_tag="pgaptmp_004456"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HGU4197841.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEMASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGIAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6261..7190)
                     /gene="metA"
                     /locus_tag="ACHKNB_RS22285"
                     /old_locus_tag="pgaptmp_004457"
     CDS             complement(6261..7190)
                     /gene="metA"
                     /locus_tag="ACHKNB_RS22285"
                     /old_locus_tag="pgaptmp_004457"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGU4197842.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7347..7790
                     /locus_tag="ACHKNB_RS22290"
                     /old_locus_tag="pgaptmp_004458"
     CDS             7347..7790
                     /locus_tag="ACHKNB_RS22290"
                     /old_locus_tag="pgaptmp_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HGU4197843.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1973 a         1979 c         1990 g         1954 t
ORIGIN      
        1 gaatgttgat tacaaacccc atcaagatga ttactcttca gctgaaaaat ttctcacgca
       61 cttcaacaaa aaatgcaaaa accagacttt ggcgttggtt tccagccgtc ctgaggggcg
      121 ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg acaagatgga
      181 atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg ctttgacggt
      241 ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt cggtttatga
      301 tcctaatgta actaacgata aaataagaat tatgagcgaa agcaaagagg atattaaaca
      361 ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt caaatgatca
      421 tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc tattgatgtt
      481 aaaaggatct gtggatgaaa taacccctcc attatcacca gcaacgatga atttgctaat
      541 ggcaattggt cagaatcacc aacttacgca actgatgatt cagctccaga aaatgccaga
      601 actacataga acagaaatgt tgactgccta taatagtgga catatgaacg ttattaatac
      661 tatttttaac gcattaccca ctctgtttaa tacgtttaaa ttcgataaaa aaatatgaag
      721 cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc gatacagcat
      781 aaacaacaaa atgttgtaga gacggtttat cttgctttat ctaaccatgc acgcctgttt
      841 ggatttaccg ctgaagatat tatggatttt tggcaacaca aagccccaca aaaatactct
      901 gcctttgagt tggcttttga attgggtcac cgggttattg ctgaattaat ccttaataca
      961 ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat tgcggagaaa
     1021 aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta aatctttgcc
     1081 tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt tgtagtgcgc
     1141 gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg
     1201 ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc gagacgctgt
     1261 accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat
     1321 tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc ccgtgacggg
     1381 tgacaccgaa gtttttaaac agcatgtcgc caggaaaaat gttagcagcg gcaagctggc
     1441 gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca
     1501 gccagatatt gagcggcacc atccgccgct cgatatagag atggcgaatt acaatctgct
     1561 caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt aatgccgggg
     1621 aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc attcggccca
     1681 cacggtcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca gacatctctt
     1741 tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc ggcagcgtaa
     1801 ataccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca ttacagccct
     1861 gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg cagccgatag
     1921 ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac tcgaccagcg
     1981 ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa acaatgctcg
     2041 cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc gtctggtgga
     2101 tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc agccaggcgg
     2161 ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg aggttgtctg
     2221 tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt ttattctgcc
     2281 agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc cagccgtgat
     2341 cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc tctggctgag
     2401 agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac cgacagtaca
     2461 cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac agccgcgtgt
     2521 aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta gtaatgcagc
     2581 gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg tttttcatcg
     2641 cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg gaagtcactt
     2701 cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca atcagtaatt
     2761 ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga tgcggcgtaa
     2821 acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc gcaagcgtcg
     2881 catcaggcat cgtgcaccga ttgccggatg cggcgtaaat gccttatccg gcctacgttc
     2941 ggcaacggct gtaggcatga taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt
     3001 gccggatgcg gcgtgaacgc cttatccggc ctacagtcag caacggttgt tgttgcttag
     3061 aactgcgatt cttcagtgga gccggtcagc gcggtgactg aagacgtgcc gccctgaata
     3121 atagtcgtca ctttatcgaa gtaacctgta cccacttcct gctggtgaga tacgaaggta
     3181 tagccatctt tcgcggcggc aaattccggc tgctgcactt tctcaacgta gtgcttcata
     3241 ccctcgccct gggcataggc gtttgccagg tcaaacatgt tgaaccacat gctgtggata
     3301 cctgccaggg tgatgaactg gaacttgtag cccatatccg acagctgctg ctggaagctg
     3361 gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg acggcgagca gttataagcc
     3421 agcagtttgc ccggatattt cgcgtggata gcttgtgcaa agcgacgcgc cagttccaga
     3481 tccggcgtgg aggtttcaca ccagaccagg tcagcatatg gcgcatacgc caggccacgg
     3541 ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc cttcactggt acgctcgcca
     3601 gtaataaatt cactgtcata cgggtcgcaa tcggaggtaa tcagatccgc cgcatcggca
     3661 tcggtacgag caaccagcag ggttggaacg cccgtcacgt cagctgcaag acgcgccgcg
     3721 accagtttct gaatagcttc ctgagttggc accaaaactt tgccgcccat gtgaccgcat
     3781 ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc ttcaatcatc
     3841 gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc atcggcaacg
     3901 atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcctgc ggaccattgg
     3961 atctgatcgg cacgacggaa ggtgttgttg atccgctcca ccacagctgg caccgagttt
     4021 gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc cgccgctacc
     4081 tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag cgcctgaccg
     4141 ccagtcagtg caccgaggct gttgatgtag ccttttttcg actcgccatg cagcagacgc
     4201 cacattttcg ccgcgcccag ttgcgccagg gtgcattcag gattgactga accgcgtaat
     4261 ttcaccacat cttccgcgct gtatgggcga gtaatgcctt cccaacgcgg ttgagtccac
     4321 tctttctgta attcttcaat ttgttgtgta cgggttttca tgtgcagatg ctccatagtt
     4381 atgtggtggt ttacgctaac aggcggtagc ctggcagggt caggaaatca attaactcat
     4441 cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc aaaacgcccc tgggagaaac
     4501 gttcttcgcc cagttcgctg gcaatgactt tcatctcttc acccagcatc tggcggaaca
     4561 aggctttggt caccggtttg ccattgctca acgttttttg atgatggatc cactgccaga
     4621 ttgaggtacg ggaaatttca gccgtcgccg catcttccat caggccataa atcggcacgc
     4681 agccgttgcc ggagatccat gcttcgatgt actgcactgc cacgcgaatg ttggcgcgca
     4741 taccttcttc ggtgcgttca ccatcacaag gtgccagcag ctgatcggca gtaatcggcg
     4801 cgtcttgttc gcgcatcact tcaagctgat ttttacggga gccgagaatg tcgttgaata
     4861 ccgccattgc cgtatccgca aggcctgggt gagcaatcca tgtgccatcg tgaccgttat
     4921 tggcttccag cgctttatcc gcttttactt tgttgagcac ctggttattg cgctcttcat
     4981 ctttgctcgg aataaacgct gctatgccgc ccatcgcaaa agcaccgcgt ttatggcagg
     5041 ttttaatcaa caggcgtgag taagcattca ggaatggttt atccatcgtc actgcctgtc
     5101 tgtctggcag gacgcgatcg ggatagtttt tcaacgtttt gatatagctg aagatgtaat
     5161 cccaacgacc gcagttcaga ccaacaatat ggtcacgcag cgcgtgaagg atttcatcca
     5221 tctggaacac ggcgggcagc gtttcaatca gcaacgtcgc cttgatggtg ccgcgcggca
     5281 gattaaagcg atcttctgca tagctgaaga cttcgctcca ccaggccgct tcctgccagg
     5341 actgggtttt cggcagatag aaatagggac cgctgccctt tgccaacagt gcctgatagt
     5401 tgtggaagaa atagagcgca aaatcaaaca ggctaccggg gattgcctca ccacgccagg
     5461 tgacatgttt ttccggcaag tgcagaccgc gtacccgaca aatcaaaacc gctggattgg
     5521 gcttgagctg ataaattttg cctgcttcat tggtatagct gatggtgccg ttaaccgcat
     5581 cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc tggtgccagt gaatcttcga
     5641 aatcggccat aaagactttc acattggcgt tcagtgcgtt gatcaccatc ttgcgctcaa
     5701 ccgggccagt tatctcgact cgacgatctt gtaagtccgc gggaatacca cgaattttcc
     5761 agtccgtatc gcgaatggaa gccatttccg aaataaaatc aggcaaagtt ccgttatcaa
     5821 tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt gcgttgtggc gtaaaatgcg
     5881 tcaccagctc agtcagaaat tctaccgctt cggcagtaag aatttgcttc tcctgctcgc
     5941 catacggcct tatgaaagcc agttcatcgg ttgttgttgc ctgttcagtc atcgtgcagc
     6001 tcctcgtcat ggatccgaaa acttccccac tgtgaacgaa ggatcgttgt gcgcttttcg
     6061 ttcagcacaa ctaagactac tcatttaaaa tgcaaaatca aaaacaattt ccattttaat
     6121 taaaaatact taataactta ttgataacaa tatgaataaa atttattcac ctcctgaggt
     6181 gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg taaggtgctg aatcgcttaa
     6241 cgatcgacta tcacagaaga ttaatccagc gttggattca tgtgccgtag atcgtatggc
     6301 gtgatctgat agacgtaata gttgagccag ttggtaaaca gcaaattacc gtgactacgc
     6361 cagctcgctc gcggtgtatt ttgcggatca ttgtgcggga aatagttata cggtacttcc
     6421 gggcctagtc cggcttccac atcgcggaaa tattcctgcg ccagcgtttg cgcatcatat
     6481 tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc tggcaaacag atatgcatcc
     6541 ccttcttccg tctctgccag aatttccaga tcggtgtaat cacgaatcaa cgctgccgga
     6601 aagtcagcat agcgcgaatg tggtgccagg aatgaatcat caaagccacg cgtcagaagc
     6661 gcatgaggat ggagaatatg atgctcgtaa acgccagaga gtttgtcggt gcgagtttgc
     6721 ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg cccagcagac aaacagcgtc
     6781 gaggtgacgt gatctttcga ccactccagc acctgtttga tctgcggcca gtaagcgaca
     6841 tcattaaact ccaccaggcc cagcggcgca ccggttacaa tcagaccgtc aaagttctgt
     6901 tcctgaatat cttcaaagtt acagtagaag ttgttcagat gctctgcggg cgtgttgcgc
     6961 gattcacggg aatcgatgcg caacagctga atatcgacct gcaaaggtga gtttgaaagc
     7021 aggcgcagaa actgattttc agtttcaatc ttcttcggca tcaggttaag gatcagaacc
     7081 ttaagtggac gaatttcctg accagacgca cgagaagttg tcatcacaaa gacgttttct
     7141 tcacgcaaga aattgacggc gggtagctcg tccggcacac gaataggcat aacctgatta
     7201 cctcactaca tacgcttata cgtttagaca tccagatagc tgaagataac cagaaaattt
     7261 gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca aggaaaaggc tacgttagaa
     7321 tataagaatg acgaaaagga gagaaaatgg ttattagtat tcgccgctca cagcatgagg
     7381 aaggggagga actcgttgcg atttggtgtc gatctgtcga tgccacccac gattttttat
     7441 cagcagagta tcgggccgag ctggaagagc tggttcgttc ctttctgccg gaagcaccgt
     7501 tgtgggtcgc ggttaatgaa cgggatcagc cggttgggtt tatgttgtta agtgggcagc
     7561 atatggatgc gctgtttatc gatcctgatg tgcgcggctg cggcgtgggt cggatgctgg
     7621 tggagcatgc gctctcgatg gcaccggaac tgacaaccaa cgttaatgag caaaatgagc
     7681 aggcggttgg gttctataag aaggtgggtt ttaaggttac gggacgctct gaggtggacg
     7741 atttggggaa accgtatccg ttgctgaatc tggcgtatgt gggggaataa gccgcccctg
     7801 ccgtgcctgc ccaggctgga gaagacgcgc cagcgtcgca cccggcattt ttttcaccaa
     7861 cgcaaaaagg ccatcgtgac tgatggcctt tcgctt
//
LOCUS       DAZFVO020000198         7849 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFVO020000198 DAZFVO020000000
VERSION     DAZFVO020000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.199"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            72..659
                     /gene="ecpA"
                     /locus_tag="ACHKNB_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /gene_synonym="matB"
     CDS             72..659
                     /gene="ecpA"
                     /locus_tag="ACHKNB_RS22295"
                     /old_locus_tag="pgaptmp_004459"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HGU4197844.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            717..1385
                     /gene="ecpB"
                     /locus_tag="ACHKNB_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /gene_synonym="yagY"
     CDS             717..1385
                     /gene="ecpB"
                     /locus_tag="ACHKNB_RS22300"
                     /old_locus_tag="pgaptmp_004460"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HGU4197845.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1411..3936
                     /gene="ecpC"
                     /locus_tag="ACHKNB_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /gene_synonym="yagX"
     CDS             1411..3936
                     /gene="ecpC"
                     /locus_tag="ACHKNB_RS22305"
                     /old_locus_tag="pgaptmp_004461"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HGU4197846.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENKS"
     gene            3926..5569
                     /gene="ecpD"
                     /locus_tag="ACHKNB_RS22310"
                     /old_locus_tag="pgaptmp_004462"
     CDS             3926..5569
                     /gene="ecpD"
                     /locus_tag="ACHKNB_RS22310"
                     /old_locus_tag="pgaptmp_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HGU4197847.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIK"
     gene            5538..6248
                     /gene="ecpE"
                     /locus_tag="ACHKNB_RS22315"
                     /old_locus_tag="pgaptmp_004463"
     CDS             5538..6248
                     /gene="ecpE"
                     /locus_tag="ACHKNB_RS22315"
                     /old_locus_tag="pgaptmp_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HGU4197848.1"
                     /translation="MFRRRGVTLNKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            6561..6890
                     /gene="ykgJ"
                     /locus_tag="ACHKNB_RS22320"
                     /old_locus_tag="pgaptmp_004464"
     CDS             6561..6890
                     /gene="ykgJ"
                     /locus_tag="ACHKNB_RS22320"
                     /old_locus_tag="pgaptmp_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HGU4197849.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7138..7752)
                     /gene="yagU"
                     /locus_tag="ACHKNB_RS22325"
                     /old_locus_tag="pgaptmp_004465"
     CDS             complement(7138..7752)
                     /gene="yagU"
                     /locus_tag="ACHKNB_RS22325"
                     /old_locus_tag="pgaptmp_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HGU4197850.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         2034 a         2058 c         2010 g         1747 t
ORIGIN      
        1 ccaggcaagt tagttttaaa aaatgactca ctgggacatc acgtcctcaa ttcaactcgg
       61 gaagaaatgc aatgaaaaaa aaggttctgg caatagctct ggtaacggtg tttaccggca
      121 tgggtgtggc gcaggctgct gacgtaacag ctcaggctgt agcgacctgg tcggcaacag
      181 ccaaaaaaga caccaccagt aagctggttg tgacgccact cggtagcctg gcgttccagt
      241 atgccgaagg cattaaaggt tttaactcac agaaaggtct atttgacgtg gctatcgagg
      301 gtgactcaac ggctaccgcc tttaaactga cctcacgtct tatcaccaac acattaaccc
      361 agttggatac ctcaggttcc acactgaatg tgggcgtgga ttataacggc gcggcagtcg
      421 aaaaaactgg cgataccgtg atgatcgata ccgccaacgg cgtactgggc ggcaacctta
      481 gcccgctggc taacggttac aatgccagca atcgtaccac cgcacaggat ggtttcacct
      541 tcaccatcat cagcggcacc accaatggta ccaccgcagt aaccgattac agcactctac
      601 cggaaggcat ctggagcggc gacgttagcg tacagttcga cgcgacctgg accagttaat
      661 ctctctgatg taccagcagg ggtagccccc ctgctttatt cctggacgga attgttatga
      721 aaaagcacct tctgcctctc gctctgctgt tttccggaat acctccggcc caggcgctgg
      781 atgtcggcga tatatcatcg tttatgaaca gtgacagcag cacgctgagc aaaacgatca
      841 aaaacagtac cgacagtggt cgccttatca atatccgtct cgaacggctc tcttcaccgc
      901 ttgacgacgg gcaggttatc tcaatggaca agccggatga gttgctactc actcccgcca
      961 gcttgctgct acccgcccaa gccagcgaag tgatccgctt cttctataag ggacccgcag
     1021 atgaaaaaga gcgctactac cgcattgtct ggtttgatca ggccctcagt gatgcgcagc
     1081 gcgataatgc caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc accattctgg
     1141 tcgtcgcccc tcgccaggcg aactaccact ttcagtacgc caacggctcc ctgacaaata
     1201 caggaaatgc gacgctgcgg atcctcgcct acggcccttg cctgaaagcc gccaacggta
     1261 aggagtgtaa agagaattac tacctgatgc cgggcaagtc gcgtcgtttt acccgtgtgg
     1321 acactgcgga taacaaagga cgggttgcac tttggcaggg tgataagttc attcccgtga
     1381 aatagatagc tgtgcagatg gataacgaca atgcctttac gacggttctc cccaggactg
     1441 aaagcccagt ttgccttcgg catggtcttt ttgttcgttc aacccgatgc cagcgctgct
     1501 gacataagtg cgcagcaaat aggtggggtg attattccgc aggccttcag tcaggcgctt
     1561 caggacggca tgagcgtccc gctctatatt catctcgccg gtagccaggg tcgccaggac
     1621 gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg gacagctacg catccggaaa
     1681 atacagctgg aagagagtga agataacgcc agtgtcagcg aacaaactcg acagcagctg
     1741 atggctctgg ccaacgcccc gttcaatgag gcccttacca tccccctgac tgacaacgcg
     1801 cagctggatc tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg cgaagcgctg
     1861 ggcaccgtac tacgctcacg tagcgaagac atcgggcagt ccagtgttaa caccctcagc
     1921 agtaatctga gctataactt gggcgtctat aacaaccagt tgcgtaacgg cgggagcaac
     1981 acatccagct atctgtcgct gaataacgtt actgcgctgc gcgaacatca tgtggtgctc
     2041 gacggctcgc tgtacgggat cggtagcggt caacaggaca gtgaattata taaagcgatg
     2101 tatgaacgcg attttgccgg tcaccgattt gccggtggaa tgctcgacac ctggaacttg
     2161 cagtccttag ggccgatgac cgccatttca gcagggaaga tttacggcct ttcctgggga
     2221 aaccaggcca gctccaccat cttcgacagc agccagtcag ccacgccagt gatcgccttt
     2281 ttaccggcgg cgggcgaagt acatctcacc cgtgatgggc ggctactaag cgttcagaac
     2341 ttcactatgg gcaatcatga agtggatacc cggggtctac cgtacgggat ttacgatgtg
     2401 gaagttgagg tgatcgttaa cggtcgcgtg atcagcaaac gcccccagcg ggtcaataag
     2461 ctgtttagcc gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg gggcggtagc
     2521 tttcatatgg atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa agagagttgg
     2581 ctagcaggtg cctcgacctc cggctcattg agtacgctta gctgggcggc aacgggatat
     2641 ggatacgata atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg gggggcgatc
     2701 aacgttaacc tgcaaaatat gctggccagt gacagctcat ggagcagcat cggcagcatc
     2761 agcgccactc taccgggagg ctttagttcg ctgtgggtta accaggaaaa aacccgcatt
     2821 ggcaatcaat tgcgacgtag cgatgccgac aaccgtgcaa tcggcggcac actcaacctg
     2881 aactcactgt ggtcgaagct gggcacattc agcatcagct acaatgatga ccgccgttac
     2941 aacagccatt attacacggc agattactat caaaatgtct acagcggtac ctttggttcg
     3001 cttggcctgc gggccggtat tcagcgctat aacaacggcg acagcaacgc caatacaggg
     3061 aaatatatcg ctctcgatct ctcgctacca ctgggcaact ggtttagcgc agggatgacc
     3121 catcaaaacg gctacaccat ggcaaacctg tcagcacgca aacagtttga tgaaggaacc
     3181 attcgcactg ttggtgccaa tctgtcacgt gccatctccg gcgataccgg tgatgacaaa
     3241 accctcagcg gtggggcgta tgcacagttc gacgctcgtt acgccagcgg aacgctgaac
     3301 gtcaatagcg cggcggacgg ctacatcaat accaacttga ccgccaatgg cagcgtcggc
     3361 tggcagggta aaaacatcgc tgccagcggg cggactgatg gcaacgctgg ggtgatattc
     3421 aacaccgggc tggaggacga cggtcagatc agcgccaaaa tcaacgggcg gattttcccg
     3481 cttaacggca agcgtaacta tctcccgctc tctccctatg gaagatatga ggtggagtta
     3541 cagaacagca aaaactcact cgacagctac gatatcgtca gcggccgcaa aagccatctg
     3601 actctctatc caggcaatgt cgctgtcatt gagccagagg tgaagcagat ggttaccgtc
     3661 tccggtcgta tccgtgcgga agacggcaca ctgctggcta acgcacggat taacaaccat
     3721 atcggccgaa cccgaaccga tgaaaacggc gagtttgtca tggacgtgga taagaaatat
     3781 cccactatcg attttcgcta cagtggcaat aaaacctgcg aagtggcact ggaactcaac
     3841 caggcgcgcg gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc atcgtgggcg
     3901 gcggtgacac agacaggaga agagaataag agttaaccta ctaataacga tgataatttt
     3961 cgcgctaatc tggccagtaa ctgagctcag agcggcagtg agcaaaacaa cctgggcgga
     4021 tgcaccggca cgcgagtttg tgtttgtcga aaacaactca gacgacaact ttttcgtcac
     4081 tcctggcggg gcgctggatc cgcgcctgac cggtgccaac cgctggaccg gtttaaaata
     4141 cactggttca ggaaccatct atcagcaaag cctcggctac attgataacg gttacaacac
     4201 cggcctttat accaactgga agtttgatat gtggctggaa aattcaccag tttcatctcc
     4261 tttaactggc ttgcgctgca tcaactggta cgctgggtgt aatatgacca ccagtcttat
     4321 cctgccgcaa accaccgacg ccagtggatt ttatggcgcg acggtgacca gcggcggcgc
     4381 gaagtggatg cacggcatgt tgtcagacgc gttttaccag tatctgcaac aaatgcccgt
     4441 cggcagcagc tttacaatga ccatcaatgc ctgccagacc tctgtgaact atgacgccag
     4501 cagcggcgca cgctgtaagg atcaggccac cggcaactgg tatgttcgca acgtcaccca
     4561 tacgaaagca gcaaatctgc ggttaataaa tacccactcg ctggcggagg tatttatcaa
     4621 cagcgacgga gtaccgactc tgggcgaagg gaacgccgac tgccggacac agaccatcgg
     4681 cagcctttca ggattaagtt gtaagatggt caactatacc ctgcaaacaa acggactcag
     4741 caacacctca atccatatat tcccggcgat cgccaactcg tcgttagcct cggccgtcgg
     4801 ggcgtacgat atgcagttca gtctgaatgg cagttcatgg aaaccggtga gcaataccgc
     4861 ctattactac accttcaacg agatgaagag cgcagactcg atctatgttt tcttctcgag
     4921 caacttcttt aagcagatgg tgaacctcgg gatcagcgat atcaacacca aagatctatt
     4981 caactttcgc tttcagaaca ccacatcacc ggagtctggc tggtatgaat tttctacctc
     5041 caacacgctg attatcaaac cccgtgattt cagcatcagt attatctccg atgaatatac
     5101 tcagacaccg tcgcgggagg gatatgttgg cagcggcgag tcggcactcg atttcggcta
     5161 tatcgtaacc accagcggta aaacagctgc cgacgaagtg ctgatcaagg tgaccggacc
     5221 cgcgcaggtg attggcgggc gctcctattg tgtcttcagc tccgatgacg gtaaggcgaa
     5281 agtaccgttc ccggcgacac tttcctttat tacccgcaac ggagctacaa aaacctacga
     5341 tgccgggtgc gatgatagct ggcgggatat gaccgatgcg ctgtggttga ccacaccgtg
     5401 gactgatatc tctggcgaag tggggcagat ggataagacc acagtcaaat tttcgattcc
     5461 aatggataac gccatttcgc tgcgtacggt agatgataac ggctggtttg gcgaagtcag
     5521 cgcttcagga gagattcatg ttcaggcgac gtggcgtaac attaaataag gccctgctga
     5581 cagcggtctg tatgctggcg gcacctttga cacaggcgat ttcggtcggc aatctgacat
     5641 tttcgctgcc gtccgagact gactttgtca gcaaacgtgt agtgaataac aacaaaagcg
     5701 cacggatata ccgtattgcc atcagtgcta ttgatagccc gggcagcagt gaattgcgca
     5761 cccgaccggt ggatggtgaa ctgcttttcg ccccccgcca actggcgttg caggctggtg
     5821 agagcgagta ttttaaattt tactatcatg gtccacggga taaccgcgag cgctactacc
     5881 gggtctcatt tcgcgaagtc cccactcgta accagacaag acgtagccca accggcggcg
     5941 aggtcagcac ggagccggtg gtggtgatgg ataccattct ggtagtacga ccacgtcagg
     6001 ttcagtttaa atggtccttc gacaaggtga caggaacggt aagtaacact ggcaacacat
     6061 ggtttaagct actgattaag ccaggatgtg attcgaccga agaggaaggc gatgcctggt
     6121 atctacgtcc gggagacgtg gttcatcagc ctgagttacg tcagccgggg aatcattatc
     6181 tggtctataa cgacaaattc attaagatta gcgattcctg tccggctaag cccccttcgg
     6241 cggactaaga cttatccatc ggtcgaggga agaattccac ctcagagctc caaaaatcgt
     6301 ttaaatgatg tggaagcgat cgtcaatgtc acctgtaacg atgggctttc tcatatgcac
     6361 accatagtgt tcaggagaca cggaaaggta tcaggcagcc tgctaatgag tggtttaatc
     6421 acgaattagt acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa
     6481 gttccccagc caaccgcggt acgttcttac atacgatgta ccgctgttct ctttacgatt
     6541 tttagctgta ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt
     6601 gtgcattttt ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtactattc
     6661 ccgccaggct cactgaacaa atatctcctt ttcaccgatg catgagcggt accaatcaga
     6721 aaaacccccg atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc tgcacgatat
     6781 ataaaaatcg atcgtccaca tgcagagaat tcgccatgtc tggtgagaac ggagtcgtca
     6841 atgaggcttg caatcgtgca agggctaaat acgggctgac accgttataa acatacaata
     6901 attaattgca ctgccccgcc agcgataata gcggggcttc gtttttcagg ggtaacaaaa
     6961 cccgatactt cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt cttcggatct
     7021 acatcatcct gaagtagcgc gatatactca ctgactatct gcatcagaat ataaaaagca
     7081 atgtttttaa cctataaaaa tggtgctgta tttgcgccat ttttatcatt caatgcatta
     7141 tctgtttgag cctaaaggga tctcagggtc tggctcatga gtaattctgt ttcgtaaatc
     7201 tctgcgaata atttcaatag accagaacca gactaaatgt ccaaaaattt cagaaacatt
     7261 ctcataccac gggagatcaa acagaggtgg cgtcagtccc atgagaggga atgaaatcat
     7321 atgaacaaaa agttgggcta aagcacctgc cagtaagccc tgccagagtt taatttttgg
     7381 aaatacttca gcgaccacac aataaccgac agcaaacact atcgaaaaga taatgtgcgt
     7441 aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat tgggatctgt
     7501 cagccccaac cagtctcgaa gaaaaatata cggtggattg agaaaattac gcgagcaatc
     7561 aatttggcct gcagccctga ttaatgattc cgggccacac gctgcattaa acatatccac
     7621 cgggctacgt ggcggcaatg gaacttcagc cccccacttc acgaatgcgg aaacaacgcc
     7681 agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg gagtttgttc
     7741 aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttc aggagtattc
     7801 ataaaacaag gcaaatgtaa agaactgtat tgttttgtat aacaagatg
//
LOCUS       DAZFVO020000199         7831 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFVO020000199 DAZFVO020000000
VERSION     DAZFVO020000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.200"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..204
                     /locus_tag="ACHKNB_RS22330"
                     /old_locus_tag="pgaptmp_004466"
     CDS             <1..204
                     /locus_tag="ACHKNB_RS22330"
                     /old_locus_tag="pgaptmp_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4197851.1"
                     /translation="ADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHADTVAFEE
                     KYGSQLELIFRFIDRALAIGVLS"
     gene            219..614
                     /locus_tag="ACHKNB_RS22335"
                     /old_locus_tag="pgaptmp_004467"
     CDS             219..614
                     /locus_tag="ACHKNB_RS22335"
                     /old_locus_tag="pgaptmp_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4197852.1"
                     /translation="MMRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTAKPRP
                     ACSDDDGLIIENCLTRLKKKKPDEYSLLVAHYLLRISKRQIARTRKKSEKAIRIEMQI
                     AEGFIDGCLSMLGVRLEMDDWLPKKVKND"
     gene            819..1016
                     /locus_tag="ACHKNB_RS22340"
                     /old_locus_tag="pgaptmp_004468"
     CDS             819..1016
                     /locus_tag="ACHKNB_RS22340"
                     /old_locus_tag="pgaptmp_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HGU4197853.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(1199..1330)
                     /locus_tag="ACHKNB_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /pseudo
     CDS             complement(1199..1330)
                     /locus_tag="ACHKNB_RS22345"
                     /old_locus_tag="pgaptmp_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1600..3582
                     /locus_tag="ACHKNB_RS22350"
                     /old_locus_tag="pgaptmp_004470"
     CDS             1600..3582
                     /locus_tag="ACHKNB_RS22350"
                     /old_locus_tag="pgaptmp_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4197854.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVQPSGDGGAVISTTSEPEYYFVIALAGQSNSMSFGEGLPLPDTY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDVSNLNHPKADLNKGQYGC
                     VGHALHVAKKLLPFMPARAGILLVPCGRGDSGFTAGAEGAFNEASGATAGSSLWGVDK
                     PLYRDLVSRTRAALKKNPKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNKYRADLA
                     DIADQCIGGTTSGVPWICGDTTYDWKAKYAVQYEAVYGGYKGKAAQNIHFVPLMTDEH
                     GANVPTNEPSEDPDIITAGYYGAASRSNGNWTTADRKTHFSSWARRGIVSDRLAGEIL
                     VRAGRLLPFLSGQSAPLATTPASTGDAQSGSAGPTQQQGAGTSAGGHTEAVTRMVAGY
                     DANSGSGVWTEQQWNASGGKGTVTDDSGRKALRLEKQPGKLTSWKMFRTVAVEEAKNL
                     LSKGGEIAVRFKIPEGVELVNGQFVFGLYWPVSQWASGATANSMLASFFLQTDASNLN
                     LMYHKGESNAQLGTFGAFDHNWHTVVFRFAGNNSEKVVPVIDDTEQSAFDLVMWTNDG
                     FTADTLTLTDITGAKATYPVLLDTVTVKVNESRASS"
     gene            3722..3916
                     /locus_tag="ACHKNB_RS22355"
                     /old_locus_tag="pgaptmp_004471"
     CDS             3722..3916
                     /locus_tag="ACHKNB_RS22355"
                     /old_locus_tag="pgaptmp_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4197855.1"
                     /translation="MTFVHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            3828..4232
                     /locus_tag="ACHKNB_RS22360"
                     /old_locus_tag="pgaptmp_004472"
     CDS             3828..4232
                     /locus_tag="ACHKNB_RS22360"
                     /old_locus_tag="pgaptmp_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4197856.1"
                     /translation="MIKGPQRKSAPVSQPEQKQKPHSFRNNLLNNPFQRENPMSEIKS
                     LVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDSILDELLGAQPEPSPELLPEP
                     QAEDVTTENGDIQPEPPVTDMTDTQPESGTML"
     gene            4308..4523
                     /locus_tag="ACHKNB_RS22365"
                     /old_locus_tag="pgaptmp_004473"
     CDS             4308..4523
                     /locus_tag="ACHKNB_RS22365"
                     /old_locus_tag="pgaptmp_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4197857.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            4528..5061
                     /locus_tag="ACHKNB_RS22370"
                     /old_locus_tag="pgaptmp_004474"
     CDS             4528..5061
                     /locus_tag="ACHKNB_RS22370"
                     /old_locus_tag="pgaptmp_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4197858.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5335..6030
                     /locus_tag="ACHKNB_RS22375"
                     /old_locus_tag="pgaptmp_004475"
     CDS             5335..6030
                     /locus_tag="ACHKNB_RS22375"
                     /old_locus_tag="pgaptmp_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HGU4197859.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVEW
                     IAGLLAAWRATAA"
     gene            6125..6256
                     /locus_tag="ACHKNB_RS22380"
                     /old_locus_tag="pgaptmp_004476"
     CDS             6125..6256
                     /locus_tag="ACHKNB_RS22380"
                     /old_locus_tag="pgaptmp_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197860.1"
                     /translation="MSDKLITPAKVLCVIVGISFSLMLVALFLSLAWVMLSSSGLLG"
     gene            6220..6375
                     /locus_tag="ACHKNB_RS22385"
                     /old_locus_tag="pgaptmp_004477"
     CDS             6220..6375
                     /locus_tag="ACHKNB_RS22385"
                     /old_locus_tag="pgaptmp_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197861.1"
                     /translation="MGDVVFVGAAGVTVTDDISRALAFAIKWVAVGIAVSPMLYGLAK
                     LVIALKS"
     gene            6387..6476
                     /locus_tag="ACHKNB_RS22390"
                     /old_locus_tag="pgaptmp_004478"
     CDS             6387..6476
                     /locus_tag="ACHKNB_RS22390"
                     /old_locus_tag="pgaptmp_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197862.1"
                     /translation="MSAELILRAMAFAIRIVAIAVLVWAIRWW"
     gene            6478..6972
                     /locus_tag="ACHKNB_RS22395"
                     /old_locus_tag="pgaptmp_004479"
     CDS             6478..6972
                     /locus_tag="ACHKNB_RS22395"
                     /old_locus_tag="pgaptmp_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4197863.1"
                     /translation="MNRVLCVVIIVMLVACGVLSLGLNHYRDNAITYKEQRDKKVSEL
                     EQANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRVNATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFTLRERLMLMQKQLEGAQEYIRTQCTKLAFYY
                     PEDI"
     gene            6969..7193
                     /locus_tag="ACHKNB_RS22400"
                     /old_locus_tag="pgaptmp_004480"
     CDS             6969..7193
                     /locus_tag="ACHKNB_RS22400"
                     /old_locus_tag="pgaptmp_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197864.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNTAKCRMVSAALQRVC"
     gene            complement(7392..7592)
                     /locus_tag="ACHKNB_RS22405"
                     /old_locus_tag="pgaptmp_004481"
     CDS             complement(7392..7592)
                     /locus_tag="ACHKNB_RS22405"
                     /old_locus_tag="pgaptmp_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4197865.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
     gene            7634..>7831
                     /locus_tag="ACHKNB_RS22410"
                     /old_locus_tag="pgaptmp_004482"
     CDS             7634..>7831
                     /locus_tag="ACHKNB_RS22410"
                     /old_locus_tag="pgaptmp_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4197866.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHR"
BASE COUNT         1909 a         1759 c         2287 g         1876 t
ORIGIN      
        1 gctgatgatc cgcatcatct gataggccat ggtcagggtg gaatgggtac aaaagcgcat
       61 gacctttttg tgctgccttt gtgcagaaaa caccacgacg aactgcatgc ggataccgtg
      121 gcatttgaag agaagtatgg ctcccagctg gagctgatat ttcgttttat cgatcgcgca
      181 ctggcgattg gtgtgctgtc ctgattttgt ggagaaagtt gatgcgtgat attcagatgg
      241 ttcttgaacg ctggggggca tgggcggcaa gtggtaacac cggggtggac tattctccga
      301 tagctgctgg atttaaaggc cttttaccat ccaccgctaa acctcgcccg gcctgcagcg
      361 atgatgacgg ccttatcatc gaaaactgcc ttacgcgcct gaagaagaaa aaaccggacg
      421 agtattcgct gctggtagct cattatctgc tgcgcatatc aaaaaggcag attgccagaa
      481 caagaaagaa gagcgaaaag gcaatacgaa ttgagatgca gattgctgaa ggatttattg
      541 acggatgtct gtcgatgctg ggtgtaaggc tggagatgga cgactggctg cccaaaaaag
      601 taaaaaatga ttagcgcggt ccgcaaaaag tatgtcagta tgttaagagt ggttactacg
      661 ccacacaact taaacccgcc gcctggcggg ttttttatga ctgaaatcgc atcagtacag
      721 taaacgtgct ggtggtggtg aataccggtc tttcagcttg ctggcttttt cgacaagagt
      781 tattggtgtg tcacgttaac cggaaaaggg aaaaagacat gctaaaacag caggatatga
      841 cagaaaccgc cagagtggtg tttaatgaat taagtgccac cgaaccggcg acagtcgggg
      901 agattgcgca gaatacttac ctttcacgcg aacgctgcca gttaatactg acccagctgg
      961 ttatggcggg tctggcagac tatcagttcg gttgttacag acgccttccg cagtgaaggc
     1021 ttttttattt gtggtaaatg ggcggctggt gggtgtggtg gttgttgctt tcccgttgct
     1081 gaaaaagaaa gcatcaggcg attagcaggg tatcagttac ccgttgaaat ttttaaatac
     1141 ctcacaattc aggcggttga ctgttgtctg gtttgcgggg agtttgttaa aagaaactgg
     1201 catggtgaat ccccctgtgc ggaggggcaa tcagcaactg gtgttttgtc accgaccctt
     1261 atcctttctg tgcgggttca ggtgctgata ctgaactcac cgggaggcac ccggcaccat
     1321 gcaatggcac atagcgccac tctccagccc ctctccggag gggctttctt atggacaaaa
     1381 aaagcccgcg ctgggagacg cgggcggcaa ggaataaaca acaaaacgtg aagtaatatt
     1441 tcagctggcg aataatatcc gacagtaatc actctgcgca atagcgcggc ctttttcgta
     1501 ttgcgggctg tagtcttcct cctgccattg tcctgtaact tccggacttc agcccgttcc
     1561 ctcatctgac tcacaacatt atcccgaccg ggaggattca tgacatttaa acactatgac
     1621 gtggtcaggg cggcgtcgcc gtcagacctt gcggagcgac tgacacaaaa actgaaggag
     1681 gggtggcagc catttggcag tccggtggcc atcacgccct ataccctgat gcaggccatt
     1741 gcggcggaag gtgatgtcac cacaccggtg gtggtgcaac cgtcgggtga tggtggcgct
     1801 gttatcagca ccaccagcga accggaatat tactttgtca ttgcgctggc cgggcagtcc
     1861 aactcgatgt cttttggcga ggggctgccg ctgccggata cgtatgatcg tcctgacccg
     1921 cgtattaagc aactggcgcg tcgcagcacg gtgacaccgg gcggtgcggc ctgtgcatat
     1981 aacgacatta ttcctgcaga ccattgtctg catgatgtgc aggacgtgag taatctgaat
     2041 cacccgaaag cagacctcaa taaagggcag tatggctgtg tggggcatgc cctgcatgtg
     2101 gccaaaaaac tgctgccgtt tatgcctgcc cgtgcgggga tccttcttgt cccgtgtgga
     2161 cgtggcgatt cgggatttac tgcgggagca gagggcgcgt ttaatgaggc gtcgggtgcg
     2221 acagcgggct cttccctgtg gggggtggat aaaccgttgt atcgtgacct ggtcagcaga
     2281 acgcgtgcag ccctgaagaa aaatccgaaa aacgtgctgt tgtcggtgat ctggatgcag
     2341 ggggaaaaag atgtcagttc ggggagacat gcagagcaca atgcactttt tcttgccatg
     2401 gtaaataaat accgtgcaga cctggcagat attgcagacc agtgtattgg cgggacaacg
     2461 tccggcgtcc cgtggatttg cggtgacacc acgtacgact ggaaggcgaa gtatgcagtg
     2521 cagtatgagg cggtttacgg aggctataaa ggcaaggcgg cgcagaatat tcactttgtg
     2581 ccgttgatga cggatgagca tggtgcgaat gtgccgacaa acgagccgtc agaagatccg
     2641 gacattatca cggcgggata ctatggtgcc gcgtcacgca gtaatggtaa ctggacgaca
     2701 gccgatcgta aaacgcactt cagctcctgg gcgcgaagag gcattgtttc agatcggctg
     2761 gcaggagaga tactggtgcg agccgggcgt ttgctgccgt tcctgagcgg gcagtctgca
     2821 ccgctggcga ccacgccagc ctccacgggg gatgcacagt ctggctctgc gggtccgacg
     2881 caacagcagg gtgcaggtac ttctgcaggc ggtcatactg aagccgtaac aagaatggtg
     2941 gccggatatg atgcgaacag tggcagtggt gtatggacag agcagcagtg gaatgcgtcc
     3001 ggtggtaaag gcactgtgac ggatgacagt ggcaggaagg cgctgcgact ggaaaaacag
     3061 ccgggtaaac tgacctcctg gaagatgttc cgtactgttg cggtggagga ggcaaaaaat
     3121 cttctcagta agggaggtga aattgccgtg cggtttaaga tcccggaggg tgtcgaactg
     3181 gttaacggtc agtttgtctt tggtctgtac tggccggtgt cgcagtgggc gtcaggcgcg
     3241 acagcaaaca gcatgctggc gtccttcttc cttcagacgg atgcatcaaa tctgaatctg
     3301 atgtaccaca agggggagtc gaatgcgcaa ctgggcacat ttggggcatt tgaccataac
     3361 tggcatacag ttgttttccg ctttgcggga aataacagcg aaaaagtggt gccggtgatt
     3421 gatgatacag agcagtctgc gtttgacctg gtgatgtgga caaatgatgg ctttacagca
     3481 gatacgctga cgctgacaga tatcacgggg gcaaaagcga cgtatccggt actgcttgat
     3541 acggtcacag tcaaagttaa cgaaagccgg gcatcatcat aaccggcaaa aaaaaccgcc
     3601 agcggcagga acggaagctg gcggaggtaa tcccaatgga gaatgtaaag aaaagatgct
     3661 ttcgtatatc ggttttttaa atgaaaacag ttctcattgt caaccataac ggtaagaaac
     3721 tatgacattt gttcatcagg tgatgctgta cttctgtacg gcggtctgtg tgctgtatct
     3781 tctttcgggt gggtacaggg cagtgcgcga tttctggcgc aggcagattg ataaaagggc
     3841 cgcagagaaa atcagcgcca gtcagtcagc cggagcaaaa acagaagccc cactcattcc
     3901 ggaacaacct tcttaataac ccctttcaac gagaaaatcc tatgtcagaa ataaaatcgc
     3961 tggtcactgc tgaggcagtg aaggaagtcc tgcgctctga agaagtccgg agcgcactga
     4021 aacagcaact tcggcagaac cttgaggcgc gtcttgatgc agaagtggat tcaattctgg
     4081 atgaattgct tggtgcacag ccggaaccat ccccggaact gcttccggaa ccacaggcgg
     4141 aagatgtcac cacggaaaat ggtgatattc agccggagcc accggtgacg gatatgacag
     4201 acacacagcc agaatcgggc acaatgctgt aacggtgagt cagggtcatc agtaaagagc
     4261 tggtggccct tttgttgttg tgagcttccg agtacgggag acggggtatg taccagatgg
     4321 aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt gggaacgggc tactggttcc
     4381 tgcagttgct ggacagggtt tccccgtctc agtgggcggc aataggcgtg ctggggagtc
     4441 tgctgtttgg gctgctgaca tatctgacta acctgtattt caaaatcaga gaggaccggc
     4501 gtaaggcggc gcggggagaa tgaactggtg agcagaaaac tccgctatgg tttatcggct
     4561 gccgttctgg cgctgattgc cgctggcgct tctgcgcctg acattctcga ccagtttctg
     4621 gatgaaaaag aaggcaacca caccacagca taccgtgatg gtgcgggtat ctggaccatc
     4681 tgccgtggag ccacccgggt ggatggtaag cctgttattc ctggcatgaa gctgtcgaag
     4741 gaaaaatgcg accgggttaa tgccattgag cgtgataagg cgctggcatg ggtggagaaa
     4801 aacatccggg tgccactgac cgaaccccag aaagcgggga tcgcgtcatt ctgtccgtac
     4861 aacattggcc ccggtaagtg tttcccgtcg acgttttata aacgaattaa tgctggtgat
     4921 cgcaggggag catgtgaggc gattcgctgg tggattaagg acggtggcag ggactgccgt
     4981 attcgctcaa ataactgtta cggtcaggta tcccggcgag accaggagag cgcgctggcg
     5041 tgctggggaa tcgacagata agcagaatat tttgctgaaa aatgacgttg gccaacgcgg
     5101 gcggataaca cgaaatcctg cgagctggca aaatgtaagt gaataaagtc aggaacatta
     5161 tttcacgcag aggcaccgta atggtgcctt tgtcatttct gcgcttcgca caagcgtaaa
     5221 taaaccaaag aacctttcag gatgagccct ggtggataac cggcagtggt ctggttaacc
     5281 ctctttgggc tggttattcc tgtgcgcagg gttcatcact aaaaggaatc aaccatgaaa
     5341 gagatgattt ctgtcgatca tgaaatatcc atgagtagtc tggattttct gaataacatt
     5401 attaatccag cccgggcaga agccggagaa gtccctcatg aaccgcgtaa gtttcttgca
     5461 aaaattgagg atgagctaga gcttgatgga accggaaaaa aattccggtt aaacaataac
     5521 cagacaagaa cggcatacta tgatctggat tttgaccaga tgatgctcgt tggcatgagg
     5581 gagtcaaagg ccgttcgtcg ttctgtgctg gcaagactca aagcgatgca tggtattcag
     5641 atcccccgga ctttacctga ggcgttgcga tttgcggcaa aactggctga acagaaagca
     5701 gtgctggaaa atcaactggc aatagcagcg ccgaaggctg aatttgttga taactatgtt
     5761 gaagcatctg gtctgatggg attccgggaa gttgctaagt tactcggtat caaagaaacc
     5821 gatttccggc tgtttttgtt ggagaacgga ataatgtatc gcctcgctgg aaaaatgacg
     5881 ccttactcgc atcacctgga tgcggggcgg tttagcgtga aaacgggcga ggcgggcaat
     5941 ggtcatgctt ttacgcaggt taaattcacc ccaaaaggtg ttgagtggat tgctggtctg
     6001 cttgctgcat ggagagctac cgcagcatga agatgataaa aatggactgg aaatttttgc
     6061 tggtctggct gattccgttt ttatgggtgg ttgcccagtt aattactgct atcaaggggt
     6121 aaagatgtca gacaaactca taacgccggc aaaggtcctg tgtgtgattg tcggtatttc
     6181 attttcacta atgctggttg ctctttttct gtccctcgcc tgggtgatgt tgtcttcgtc
     6241 ggggctgctg gggtgacagt gactgatgac atcagcagag cgctggcttt tgctattaag
     6301 tgggtggctg ttggtattgc tgtgtctccg atgctgtatg ggctggcaaa actggtcatt
     6361 gcgctgaaat cgtgaacttt aaaaagatga gtgctgaact tattcttcgg gcaatggcat
     6421 ttgccattcg tattgtggcc attgctgttc tggtctgggc aattcgttgg tggtgatatg
     6481 aaccgtgttc tgtgtgtggt gattattgtc atgctggtag cctgtggtgt gcttagtctg
     6541 gggctgaatc attaccgtga taacgccata acctacaaag agcagcgcga taaaaaagtc
     6601 agtgagctgg agcaggcaaa tgcaaccatt actgatatgc agcagcgcca gcgtgatgtt
     6661 gctgcacttg atgccagata ctcgagggaa ttagccgatg cgagagctga aaatgaaact
     6721 ctgcgcgctg acgttgccgc tggtcgtaag cgcctgcggg tcaacgccac ctgccccggt
     6781 accgtgcgtg aagccaccgg cacctccggc gtggataatg caaccggccc ccgactggca
     6841 gacaccgctg aacgggatta tttcactctc agagagcggt tgatgctgat gcagaagcag
     6901 ctggaagggg cgcaggaata tatccgcact cagtgcacta agctggcttt ttattatccg
     6961 gaggatatat gaagaaatta cgggtaaccg tagaaccttt tcagggaaca attccgttcc
     7021 gtattttgca gcgtggtcgt gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat
     7081 tacactcccg gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga
     7141 tgaataccgc taaatgccgc atggtatccg ctgcattaca gcgagtgtgt tgagcgacct
     7201 tattatccat gcgcggtatt gtcgccgtat ccccgcatta acagagaccg cagcccgacc
     7261 gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa cgatacacac cggggtttac
     7321 cgcgttaacg gagcgcggcg ttgtcccctc atagtcgcct gtccggtgcg atggtggaag
     7381 aaaccgaacg ttcatttctc gttatttttc atgctggccg ggcgcagatg cgttgcatct
     7441 gttgccagcc ttctcctgca ggcttcaata acccacgctg aaaagttacc ggacccttta
     7501 tgctcaaggg ctatgttgat ttgttcaatt atgtgattgg ggaaacggat attgcgggtt
     7561 gtggttctgc gggtccggtt tttcgatgac atatttattt cctttactga ttgtcatatg
     7621 acggggattt tacatggctc agcttcgtac actccagagc agaatcaaaa cactgaatac
     7681 ccgacgggtg aatattctga agggggagca gcgtcgtgtc agtggcagtg cacgtgtttc
     7741 cctcaagcgt catatctggc tgagggacgc cgggcagtgc tgtatctgtg gtcgtgtggt
     7801 tgacctctgt gacagtgaac tcgatcaccg a
//
LOCUS       DAZFVO020000200         7721 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFVO020000200 DAZFVO020000000
VERSION     DAZFVO020000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.201"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..610)
                     /locus_tag="ACHKNB_RS22415"
                     /old_locus_tag="pgaptmp_004483"
     CDS             complement(<1..610)
                     /locus_tag="ACHKNB_RS22415"
                     /old_locus_tag="pgaptmp_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HGU4197867.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFT"
     gene            complement(656..1486)
                     /locus_tag="ACHKNB_RS22420"
                     /old_locus_tag="pgaptmp_004484"
     CDS             complement(656..1486)
                     /locus_tag="ACHKNB_RS22420"
                     /old_locus_tag="pgaptmp_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4197868.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(1490..2962)
                     /locus_tag="ACHKNB_RS22425"
                     /old_locus_tag="pgaptmp_004485"
     CDS             complement(1490..2962)
                     /locus_tag="ACHKNB_RS22425"
                     /old_locus_tag="pgaptmp_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4197869.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            complement(3011..3886)
                     /locus_tag="ACHKNB_RS22430"
                     /old_locus_tag="pgaptmp_004486"
     CDS             complement(3011..3886)
                     /locus_tag="ACHKNB_RS22430"
                     /old_locus_tag="pgaptmp_004486"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HGU4197870.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(3919..4836)
                     /locus_tag="ACHKNB_RS22435"
                     /old_locus_tag="pgaptmp_004487"
     CDS             complement(3919..4836)
                     /locus_tag="ACHKNB_RS22435"
                     /old_locus_tag="pgaptmp_004487"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HGU4197871.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            4954..5506
                     /locus_tag="ACHKNB_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /pseudo
     CDS             4954..5506
                     /locus_tag="ACHKNB_RS22440"
                     /old_locus_tag="pgaptmp_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5740..6426)
                     /locus_tag="ACHKNB_RS22445"
                     /old_locus_tag="pgaptmp_004489"
     CDS             complement(5740..6426)
                     /locus_tag="ACHKNB_RS22445"
                     /old_locus_tag="pgaptmp_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4197872.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(6538..7485)
                     /locus_tag="ACHKNB_RS22450"
                     /old_locus_tag="pgaptmp_004490"
     CDS             complement(6538..7485)
                     /locus_tag="ACHKNB_RS22450"
                     /old_locus_tag="pgaptmp_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HGU4197873.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            complement(7599..>7721)
                     /locus_tag="ACHKNB_RS22455"
                     /old_locus_tag="pgaptmp_004491"
     CDS             complement(7599..>7721)
                     /locus_tag="ACHKNB_RS22455"
                     /old_locus_tag="pgaptmp_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGU4197874.1"
                     /translation="IDGWLKVLKSDKKALFRACRQARKASEYLLTRRTSQECAA"
BASE COUNT         2285 a         1960 c         1421 g         2055 t
ORIGIN      
        1 gggtaaacac cggtgacatt ttatattgca gaacaaatgc attttcagtt gctgtttttt
       61 tatgattccc atatttgaaa ttattaacct tcgtatatac cgtggtctgc catgcaaaca
      121 accatgaatc attaactttg tatgtcacat aaccatcaaa ctgatgctga tcattacgcg
      181 ccctgttgcc atctaaatcc gtctgtcgga aagctttcca gtcatagcga tagcgtaagg
      241 ccagattaag atctggcgtt gcctgatacg aaattttcag ataagggtta tactgacttc
      301 ctgctgacat ccattgtgtc tgaatccctg ggattagagc ccatttgtca tctattttat
      361 atgttttatt tatatctatt ttattataat ttagtgcaaa caattcattt tcattgttgt
      421 gaattgtatt ccaggtattt gactcaaaac ttgcccacca accattattc cacccctgac
      481 taacctgaat caacgtttca taagcgtgac tggcgcttct gtatcctcca cgtagattaa
      541 gagttaatgc ctgagagcac aaaggtaaac caagaagaat cacagttaat agtggataat
      601 attttttcat aaatgcctca tcattaaata tatttatcac ttgtaatctg atttctcaat
      661 gaaacttatg ttccgcaagc cattccagaa gcaggctttg ccatttttct atcggatatc
      721 cattttttcc ggatacacca aatccatgcc ctcccttatc aaaaaaatga gcttctgcat
      781 gatgtccctt atcccggata gagttaaaat atctcagaga gtgaataact ggaacatcct
      841 tatcatctgt tgcatgcaca ataaagactg gtggcatata ctctgtaaca ctattttcaa
      901 gactggctga atattcactt tcacctgcat ccagaagacg tttccggcat ccctgatgag
      961 caatactcct gtcaagagat ataactggat atatcagagc catccagtca ggacgcacag
     1021 aagtctcttc ctcataatgg agatgagtaa tatatccagc aagatgacca cctgccgaaa
     1081 accccataca tccaacactc ttaaatgttc tctccgacat tccggcccag taagaaataa
     1141 agttcagggc attcttcgca tccgcaaagg aataataacc attaacggag ggcattctgt
     1201 aagaaagaat aaatgccgta tatccctgac ctgctatcca tctgcctaca tcaagtccct
     1261 ctctttcata aacaatcctc ctgtatccac ctccaggtat aatcagcaca ctatgttgat
     1321 tagatacaac aggctcaaca actgtaataa ggggcacaat acctgtatat attatgccat
     1381 cgtcagtatt ctcaaatagc gaattgtcag ttatgtaacg ctcataatta tcaagagaat
     1441 atatattctc cctgtaatga atcatttttt tattccctga tagcatcctt tattctcctg
     1501 caacattctt ttttcttgct ccataaaaag gttttccatc gacagtatca tattcaagcg
     1561 cagcttctgg atggatccat ctctgtacac gtgatggcat atcaaaacca ataaggacaa
     1621 gaacaacaaa tgttaagata aaagcaagag aacacattgc tgtaccaaga cttgtatgag
     1681 acgcaacaac tgcaccaaga ataggcgcac atgctccacc aagtgaacct acattataaa
     1741 taaaccccag tcctgccgca cgctgctcca cattaaaata accactgatt attttcggca
     1801 ccagagcacc aatcccctga ccaaaaacct gctgagtaaa gatcagaagg ccaagtaacc
     1861 atacatagtc ccggtccaca agaaaaacgg ggataataac aagctgaccg ataagaagtg
     1921 atataacata tgcttttcgg gttccaagcc agtcccccat aaaaccacct atacaacttc
     1981 cagctgcaga gccaaaacct gccagcataa atatgagagc aacagtttct ggtgaatact
     2041 gcatatcaac cttaagccat gtaggaagaa aagcctgcag tggccatccg tagaggaaac
     2101 agaacataac cacaagcatc aacataatgc ctgttggcca tcttttcccc ataccctgat
     2161 atataaaact gataaaaaca gccgcacagc acaatgccat aactgtgatt aaagcagcgt
     2221 ttgcagtgac gcctgaaaaa caaacatata gacatgcaaa tgcaatacaa gacagaagaa
     2281 tatttatgta tttattcttt tctctgtaca gaatatccac cattgttctg acgggtttat
     2341 tctcttgttg ccttttttgc cagtcatcag actccggaag attcttgcga agataaaaag
     2401 caaataatat tggaagcatg ccgacaaaga aaagagaacg ccagccccat agtggtacta
     2461 caatgctata aacctgtgca gctaaacctc ccccaataga aaatccagaa attaaaaaac
     2521 cacttgcctt gtttctgaga tgaacaggcc agctttcgat aacatatgtt acacttgagc
     2581 catattcacc tgccatacca agcccaataa caatgcgagc aataaacatc actgcatatc
     2641 caggagccag accacaggca agggttccga gagagaatag cactatactt aaaaccattg
     2701 ccatcctgcg ccccatcttg tccgataaag cgccaatagc aagcccccca aaccatcggg
     2761 aaataaaagc agcggaaata aggcttgccg cttctattgt cgtcaagccg aattcatgct
     2821 gaacctctgt aagaaccaga gatatcagaa cgaaatcaaa accatcaagt aaataacctg
     2881 tccatgccgc accgaatatc ttccactgtc tgggtgaaag atgcttatac caaacctgct
     2941 ttttctgaat acaggagtcc atattaaacc tcacaataat agcggattta taatacactg
     3001 gagtattact ttaagtaata caatcccggt cccaaagagt tgcaccaata agaccagagt
     3061 cacttctgaa atatgccgga aacagcatta cattacaaat ttcagaatat atggaaagat
     3121 gtttcgacac tttctgaata tacccatcag caagtccaac acttcctccg acaacaacaa
     3181 cctgacaatc aagcagtaat tttaaactta ctattaattc agcaatagta ctggcagaac
     3241 gctctgtcaa aaattcggct tccttatatc cctgtctcgc taattcaaaa atatatttgg
     3301 tgctatatcc tgcaagttca ttttttgctg cccccatgat ggcccgacca gatgaaaccg
     3361 cttcaacgca tccccgacga ccacatccac attctgtatc cgttactccg gacaatatat
     3421 gcccaacatg tccggctaat ccagaaacac ctgttaataa ttttctgtta actacaattc
     3481 cccctccaac ccccgtagaa acggttataa aaaccatatc acaaatttct tttggcaaaa
     3541 ctgtatattc tgcccatgcg gctgcctggg catcatttat cacacttcca tttaaaccag
     3601 ttatatcttc cattatattt ttcagaggat actctttcag ccctccaaga ttgtctggat
     3661 tcaaagctgt caatactcca ttattaataa tccctgttga tgctactgca aatcgctccg
     3721 ctgttccctt catttcagaa accagtagac gtaaagcccc tgtcatttcg tcaggacatc
     3781 tttcatgagg tgttgatatc tgttttttca gcaaaaatga accgtcatca gatatcaacg
     3841 ccgctgaaat tttcgttcct cctatatcaa cagccagtgt tatcattcat tttacctata
     3901 taacaatctt gcaattattc agattatttt cattttttta tttctgtcat gttcttcgag
     3961 cagtcgctct gcaagttttg ataatacatc cagattcttt tcctttaccg gagaaaaagg
     4021 acttctgcat acaggagtat ttataatccc catatagtaa agaagagttt ttatacctgc
     4081 caggacacca ctctgaataa gagtatcaat aacctggtta caggccacct gcatttcctt
     4141 cgccttaatc acatcattat ttttgactgc ttcaaatatt tcaagataac gccaccccat
     4201 tatattatat gtactaccga ttcccccatc tgcacctgca attaaccctg atgcaaatat
     4261 ttcatcgtaa ccattatata acacaagctc tggatgattc cttttgattt gttccatctg
     4321 gaataaatca ccggatgtct gttttaatgc acaaaccccg ggtattgtga caagctcatt
     4381 tatctgatct aaagaaaaac gcactccgct aagagccggt atattataaa ctaccatcgg
     4441 aagtccatct gcagaatcaa taatcttcct gtaatggata caatgctctt caaatgaaaa
     4501 aggataataa aatggagtaa cagcagatat agcatgatat ccgtattttt ttgcagcttt
     4561 tgcaagtacc tcgctttctg ccgtactgat tccgcccaca tgcgctataa gtgttagtct
     4621 tccatctgat tcatcggcta ctgtttcaag aattttttct cgctctgcaa cattctgaag
     4681 aaatgcttct ccagtagagc ctccaacata caatccctta atattttgtt cgatattaaa
     4741 acggatcagt tttcgaagac tctccatatc gatttgctgg gattggtcat aaggagtggg
     4801 taatgctgat atgacccctt taaattcaca ttgcattttt aatcaccttc acttaactgc
     4861 ataatgggat acctagtaca ccatcaagta caaagattat ccaacagaaa agaagcacaa
     4921 tgtgaagatt atcaaaaaaa agccatcaga aagctgtcat gccgctcagt taaggatcgg
     4981 ttgatcgatc cagtgtccag ttactattct ctgcgtaacc ctctgcgact atcttgccct
     5041 ggaactgatt acccacagcc ctgaagagtc cgacactgtc acgctacaca aacggcgtct
     5101 tcctctggaa atgatggtct ggtgaattat cagtatggcc catgagcgta aggaaccttc
     5161 atcagattgt gaaccggctg gacatcatgc tgccaggcag tagcgccgtg ctccatgccc
     5221 gccagcggcc aggaagcgga gccatacgct gcgtgttcac tcaaacagcg cagctctagc
     5281 ataacatcac gccatatccg ctctggtgcg atctgaatct gctgaccatt gatggcgtgt
     5341 tctgacgaac acaggacacg caagaacacg ataaagcttc actcgccaaa tacatagtgg
     5401 aaaccagaca ctttatcctc aggtaaaaat ggtctgtctg atagaactga ccagccatct
     5461 actgacggca gcggccttcg atacaatgaa tctggcacta aataagactg gcaatatagc
     5521 cgataatgcg ttgaatcagt ggctatatcg tatacataaa acagacatca ttattctgtc
     5581 ttgttgtcca acacataaaa actaataaaa acataccatt ccctgtcgtc agagaattta
     5641 ttgataaaca ggctttattg aacaaatcaa acattcaggg aactggcagt tgaacaaaac
     5701 gccagtccga agactggcgc agtgataaaa cattctgcat catgccacat tctgctcctg
     5761 aagatgacgc agacgatggt cccagtttcc caggtaatgc ccgggagtca gcagtacgcg
     5821 gcgtcgctca ttacagagcg agttaccaat caccacttct ccggccacac cacacaacga
     5881 cagttgtata tacgccatgc cggctgccag cgggtcaata tccgttgccg acacccacat
     5941 cctgtggtat gccggatacc ccgaacggtt caggatgtca gccattgcca gaatcatact
     6001 gccggcacca caggcaggtt cactgagcgt gataaagggt ttgttctcaa acaacgcctt
     6061 cacatctccc atctgcatct gtgccatcat gctggcaata ctccagggcg tgaaaaactg
     6121 actccggtat ttatccccca gttccagttg catgaacaca ctccccagaa aatcacagaa
     6181 tccctgctcc agtccctcaa tgacatgagc aagcaaatgc gcaatgtgga taacatcctg
     6241 cttttcataa ccactgacga ttcgcagata cttctgttcc cgggcctcac tgaactgaag
     6301 ccggttttcc agggcaatca cactacagct gacaaagtct tcaaacacct tatgacgttt
     6361 gtgataacag gctgtccggt taaacaacga aatgaactcc ttctgatggt cagtcatggt
     6421 ggccatgttt tctccgtaaa aacagggaac acatggcccg gacaggggaa caggtgttcc
     6481 ccttcgggtt gaaatgttga taaaacgggc atcacggaca tgatacccgg aggttaatta
     6541 ctgggctgag accagaggaa ccggtaaagg tgcggctttc gtctgttctt cagcgcactc
     6601 ctccaccaga tacagtcggg gctgtggaag tgtttccctg cccggccatt cggcctgcag
     6661 atcactgtcc gtatgctctc cgccatcgta acagttgtaa ccctgtatac cgtgtaccgg
     6721 ctcgctgtac cagtgtcgga cctcgcagtc aaacaccttc gagatctcac caaccagttc
     6781 gcctgaaggc ggataccagg gcgtgtcgat cagcagcacc agactgctca tctcatcacg
     6841 cgcatggcga acattatttc ctgacggcca ctccacaccg tatatccggc tgtaaaggct
     6901 ggtcgttgtg gaaatacctt gcagcagtcc acctgaacca ttcagctctg tagccagacg
     6961 ggagggtatg accatcagca tgtcacaggg ctgcgcctgt tccgggtaat ccgacaacca
     7021 gctccagcag gcttctgcat caatgtgttc actcagcccg gccagaccaa accagtcaga
     7081 atactgacgg ctcatcagcg tggtgatgat ctccctggca ttatccggca gattacccca
     7141 gcgaacagcg ccgatacatg actggtgata aagacgctca atggtcttta tcgtttcacc
     7201 atccagaacg gcatttttta ccagcaaccc cagccactgc tcaaacgcct ggttagccgg
     7261 agatgacaac cccgttccgt gacgaaccag cgccggatat ggcggatact gcgtcgtccg
     7321 tgtgggtttt accagccctg cacaaccggc cagaaacagc tgtatactct gctgtatggc
     7381 atgacggtag cgcggggtct cacgcccttc aatccactgt gtaagtacat caagacagac
     7441 tgatttaccg gtaatttcaa aacggttttt gcaccattca gacattgttt acgctcctct
     7501 tttttataca gatgtaaaaa gagggcattg tcccgtgagg tacaatgccc tcacgggata
     7561 aatggcggtc aggttataac cgccaccggt agtaaaaatt atgctgcgca ctcctgcgaa
     7621 gttcgccgcg tcagtaaata ctctgatgct ttacgtgcct gccggcaggc gcgaaacagc
     7681 gcttttttgt cagacttcag aaccttaagc catccgtcaa t
//
LOCUS       DAZFVO020000201         7673 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFVO020000201 DAZFVO020000000
VERSION     DAZFVO020000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.202"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHKNB_RS22460"
                     /old_locus_tag="pgaptmp_004492"
     CDS             complement(103..366)
                     /gene="cedA"
                     /locus_tag="ACHKNB_RS22460"
                     /old_locus_tag="pgaptmp_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HGU4197875.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            549..2810
                     /gene="katE"
                     /locus_tag="ACHKNB_RS22465"
                     /old_locus_tag="pgaptmp_004493"
     CDS             549..2810
                     /gene="katE"
                     /locus_tag="ACHKNB_RS22465"
                     /old_locus_tag="pgaptmp_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HGU4197876.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHKNB_RS22470"
                     /old_locus_tag="pgaptmp_004494"
     CDS             complement(2857..3615)
                     /gene="chbG"
                     /locus_tag="ACHKNB_RS22470"
                     /old_locus_tag="pgaptmp_004494"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HGU4197877.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHKNB_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /gene_synonym="chbF"
     CDS             complement(3628..4980)
                     /gene="celF"
                     /locus_tag="ACHKNB_RS22475"
                     /old_locus_tag="pgaptmp_004495"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4197878.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ACHKNB_RS22480"
                     /old_locus_tag="pgaptmp_004496"
     CDS             complement(5085..5927)
                     /gene="chbR"
                     /locus_tag="ACHKNB_RS22480"
                     /old_locus_tag="pgaptmp_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HGU4197879.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ACHKNB_RS22485"
                     /old_locus_tag="pgaptmp_004497"
     CDS             complement(5935..6285)
                     /gene="chbA"
                     /locus_tag="ACHKNB_RS22485"
                     /old_locus_tag="pgaptmp_004497"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HGU4197880.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6336..>7673)
                     /gene="chbC"
                     /locus_tag="ACHKNB_RS22490"
                     /old_locus_tag="pgaptmp_004498"
     CDS             complement(6336..>7673)
                     /gene="chbC"
                     /locus_tag="ACHKNB_RS22490"
                     /old_locus_tag="pgaptmp_004498"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HGU4197881.1"
                     /translation="LEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLINNV
                     FLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEE
                     RKVDALAAELLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIV
                     RRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTP
                     LASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAA
                     LAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQI
                     NEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAF
                     FNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2054 a         2013 c         1714 g         1892 t
ORIGIN      
        1 tcaaaggcag ctttttgtta caggtggttt gaatgaatgt agaaacgaaa tgcagaattt
       61 caggtcatgt aactcccggc aaaaccggga ggtatgtaat ccttactcag tcacttcccc
      121 ttcctggcgg atctgatttg cccaacgttg ggcagattca ggcacagtaa acgccggtga
      181 gcgcagaaat gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac
      241 atcacgaaac gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac
      301 atagctaata atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa
      361 acgcatatta aaaattagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct
      421 ttgcgtgtat ttcataacac cgtttccaga atagtctccg aagcgggatc tggctggtgg
      481 tctatagtta gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga
      541 cgagttcaat gtcgcaacat aacgaaaaga acccacatca gcaccagtca ccactacacg
      601 attccagcga agcgaaaccg gggatggact cactggcacc tgaggacgga tctcatcgtc
      661 cagcggctga accaacaccg ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc
      721 ctgatacacg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt
      781 atgcgctgac cactaatcag ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg
      841 gtaaccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg
      901 accatgagcg catcccggaa cgtattgttc atgcacgcgg atcagcagct cacggttatt
      961 tccagccata taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca
     1021 aaatcacccc agtatttgtt aggttctcta ccgttcaggg tggtgctggc tctgccgata
     1081 ccgtgcgtga tatccgtggc tttgccacca aattctatac cgaagagggt atttttgacc
     1141 ttgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg
     1201 ttcatgcggt aaaaccagaa ccacactggg caattccaca agggcaaagc gcccacgata
     1261 ctttctggga ttatgtttct ctacaacctg aaactctgca caacgtgatg tgggcgatgt
     1321 cggatcgcgg catcccccgc agttaccgca ccatggaagg cttcggtatt cacaccttcc
     1381 gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag
     1441 gtaaagcctc actcgtttgg gatgaagcac aaaaactcac cggacgtgac ccggacttcc
     1501 accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct
     1561 tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacca
     1621 aactgatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca
     1681 atccggataa cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc
     1741 ctggtctgga cttcaccaac gatccgctgt tgcagggacg tttgttctcc tataccgata
     1801 cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac cgtccgacct
     1861 gcccttacca caacttccag cgtgatggca tgcatcgtat ggggatcgac actaacccgg
     1921 cgaattacga accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga
     1981 aacgcggcgg ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgtgagcgca
     2041 gtccatcgtt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat
     2101 tcgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt
     2161 atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg
     2221 tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg
     2281 acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg
     2341 tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg
     2401 ccattctcaa ggccctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg
     2461 gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcac
     2521 cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca
     2581 acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg
     2641 cgggagacgc gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga
     2701 ttgtggaagc tgacagcgct gacggtagtt ttatggatga actgctaacg ctgatggcag
     2761 cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tccggcgtaa atatggataa
     2821 ggtcacgtcc cctggggagt gaccttacca ccttacttac acatcaagat aactacccaa
     2881 ccgataacca cgctcagcaa ttgcatattt caacgacgct gatgtcagca cttccagctc
     2941 cgttaaccgt gggaaacagt aagcactctt gcgaatcaaa ttatcgacaa acgccggatg
     3001 gcacatgact tccagcgagc gttccctgcg atggctggaa tcatccagca cttgcaggaa
     3061 cagtgcctcg ctaatctctt caccataaaa cgcgctactg aatccctgcg aactgcgcag
     3121 attagccggt aaatcaccag cgttagatag cggctgacgg tcgatgcgca acgcaatccc
     3181 ctcttcagcc gcaaacctga caacaatcgg gaaaatctgc gggaacatat gcacatgatg
     3241 atggctatca agatgcgtag gtttgcgtcc aaatagctca atgaaacgca aatactgact
     3301 ggcaagctcc tgagtaattt cttccagcgg taaagcatct tcttctgcca actgccagat
     3361 ccattttccc agcacaccat cgcgggttaa ccccggcata gctgtcagtg gcttgcccat
     3421 agtaaggaca aagtgcatcc ctatggccag actcggttca tcacgactca actgcaccgc
     3481 atggtcaata gcctgcccat tcaccagcgc cgtcgtcgat gtgacaatcc cattgcgaca
     3541 ggcctcgata atgccgtagt tctgaccttt gcttaagcca aaatcatcgg cattaacaat
     3601 cagtaagcgt tccataatca gcctcggtta atgtgctttt ttaagctctg cgatgcagtc
     3661 ggcaaaattt ggcagccatt tctcgtgcgc cagaatcatc tcgcgtgcca gcagctcagc
     3721 atcgcgatcg gaatgcacca acggactaag gtttagcgcc agtaacacat cgttaaattc
     3781 tccgctaagt gcggcgttgc tggcagcaat ctcgaagcct ttaatggtgt gaatcagccc
     3841 catcacttta tcatcgaaat gcgtaatgcg cggatgtggc gtcgcgccat cgcgccccag
     3901 cttacaggtc atttctaccg cccaatctgc cggaatatta tcaatatgcc catgatgcgg
     3961 gatattaacg taatgttcag cttgcttgtc gttgtagata gcgttgatca cttcgcacgc
     4021 tgcatcagag taataagccc caccgcgctg ttccagttct ttcggcttaa ctttcaactc
     4081 cggattttta tacagctcaa aaagttgttt ctcgactttc tgtactacct gtgctcgtgc
     4141 gccgcctttg tagtattcgc ccatttcaat agccagcatc tctttctgct tgaagtaata
     4201 cagcagataa gaacatggca gcagatttaa cgaacgaatt aagccctcac taaatggcag
     4261 atcgaaaata ttttttacag aggacgcttt taactgccct gacgccacac catcaagcaa
     4321 ttcggcaaag cgcgacttgc catttaccag cacatcctta atgaacacca tatggttgag
     4381 gccgaacaga tcgatagata aatcatcgct gtctttcagc atcagaacat cgcgaataaa
     4441 catcttcatg ccgatcggaa tattacacac gccgataaag cgtttaaatc cggtatgacg
     4501 ataaacggct tcagtgacca ttcccgccgg gttagtgaag ttaatcaccc atgcattcgg
     4561 acaaagttct tcgacatctt ttacgatgtc aaaaatcacc ggaatggtac gcagaccttt
     4621 aaacagaccg cccgcgccgt tggtttcctg accaagataa ccatgactta atggaatacg
     4681 ttcatccagt tcgcgcgccg gtaattggcc tacgcgcagt tgggtagtaa cgaaatcagc
     4741 atctttcaat gcttcgcggc gatccagcgt tttataaagc ttcatcggga cgccagcgtt
     4801 atcaatcatc cgttggcaga gttcaaaaat gatatccagt ttctctttac catcttcgac
     4861 atccaccagc cataattcgc tgaccggcaa ttcgtgataa cgcttaataa atccttccag
     4921 taactccggg gtatagctgc tcccgccacc aatagtgacg acttttaatt tctggctcat
     4981 aatttctccc ttcagtacag aatactgata tctggcatat ctgccccccc ggacataaat
     5041 aatccagcaa cagtacagat atgtgaattg tcaggtataa cgacttactg attaaattca
     5101 gtcaatttct tacgatagct cttaggcgta aaggacgtta atttcttaaa cgttttaata
     5161 aacaaactcg ggctactata accggcctca aacgcaatat ccgtcacgga atagttggtc
     5221 atttccagtt gttttttggc aaaattaata cggatctcat taataatctg cattggtgtt
     5281 ttgccataat atcgttgagt cgctcgcgtc aaatattcct gtgatttggc tgacaacgtc
     5341 accatattct ccagcgccga ttcactaaac tgctctttat catgcatctt ttctaccgta
     5401 cttttcagcc actgcggtac atcatcaatc acctgttctt cgcggtaatg acgtaaacgg
     5461 ttaatgacat aaaaagtaac catctcaaca aactcttcca gccctgtttc gcggaaattc
     5521 aatgaagaaa tcactgtttc cacataggtg agaaaagcgt tattggtccg gtatacctgc
     5581 gaagcgacaa agcaataagg caacaacggc aggtaatgct gctcaaaaaa gcgtttactg
     5641 atcccaacgt tcaatatacg cgtggcacca aactcataaa aactttgatg gtgcgaacct
     5701 aacggaataa aaacaaaatc gccccgttcc agtaacacgc gcttaccgtt aatctcctgg
     5761 aaataacgcc cggttaatac cagagtaaat tcataatagt cgtgctgatg cagcccgctg
     5821 atactctcag ttttgttata gataaacaca tggaagtttt tgccattaaa caactgctgt
     5881 tctcgggcag tggcaatttc cggcgcgtta atcactggct gcatcatcga ctccttatgc
     5941 cttcagtttt tcatgaagct caattaattc agtaatcagt tcacgcgcaa gcatggacgt
     6001 cattaaatga tcctgagcgt ggaccagcac cagactcact ttcatcttac cttcgcccgc
     6061 atcgccttca atcagtttcg tctgtaccag atgcgcttca ttcaatgcca tccgtgactg
     6121 atccatcatg gcttttgctg cagcaaaatc gccctgcttc gcctgtttca gtgccgcata
     6181 cgccaggctg cgcgcttgtc cggagttgat gataagcccc atcaccactt cttccagctc
     6241 ttcagcttcc gtttgcgtat cgggaatgtt atcgagatcc atcatacatc gttcctctct
     6301 attcttaccg gcacgattac ccgtaccggc atcgattaaa atttcagggc attagcgata
     6361 tcttcttcgc tctcttcttt atcaatcgcg ttttgtgctt tgttagccac cacaacaaag
     6421 ggcagataaa ttaacgttgc gataccaagg ttgaagagtg cgaccagcaa tgcggcgacg
     6481 ctaccgttgg tgttaaagaa ggctcccaga ccggttggca tggtccacgg tgcaatattg
     6541 gtcaccggag gaatgatgcc catgtagtac gctgcgaggg tgattgccgc cagaatcggt
     6601 tgtaccagta caaacgggat aaacatcacc gggttcatga taattggcag accaaacaga
     6661 atcggttcgt taatctggaa gatgccggac ggcagcgcca gttttgctac ctgacgataa
     6721 tcagcacggc gagaggcgat aaagatagcc aggatcaggc ctaaagtcgc accactgccc
     6781 ccaaggaaaa taaaggagtc cagcatcggc ttggcccaga tatggaaggt cttaccggct
     6841 gccagcgccg cttcgacgga accatattgc tgataggtcg cgatattttc cagcgcccac
     6901 ggcgtcataa tgccgttgtc cagtgcggtc agcgccagcg cgccatgaat accgaagaac
     6961 cagagcagtg gaacaaagat cacataggcc cagcccacca cgctacccaa cgatgccagt
     7021 ggggttgaga tggtatccat aatgatctga tggaagttgg tgccccaggt attcaacgcc
     7081 caggcaataa tccccatcac ggaaagaata ataaagccgg gaattaatgc cgagaaggaa
     7141 cgcgatactg aagcaggtac gctgtcgggc agtttaatga cccaattgcg gcggacaata
     7201 aaggtaaaca tttctgccac caccaggcca ataataatcc cggagatgat attcgcccca
     7261 cctaaccagt ttgcgccaac cgcataggcc tcaccgacac tatatggggt gacggtcata
     7321 aatgctgcaa cggataacaa ctcagccgcc agcgcatcga ctttacgctc ttctgccagc
     7381 gccatgccaa taaagaacgg tgccattaaa gacattatcc ctaatgttcc gttatatacg
     7441 ttgccgccaa tacctttcag accattaagt gtttcaatgg ttgaggcgtc gaggcgaata
     7501 cctaaggaat aaaaaaacga cccctcccca aagcttagaa aaacgttgtt aattaataca
     7561 aacatggccc ccgcaagggt taacggcatt aagcgaataa agccattttt gattgcatta
     7621 acgtgtggct gctttcctat tttaactgca aaagggagga gtaccttttc aag
//
LOCUS       DAZFVO020000202         7647 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFVO020000202 DAZFVO020000000
VERSION     DAZFVO020000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.203"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(179..1552)
                     /gene="cysG"
                     /locus_tag="ACHKNB_RS22495"
                     /old_locus_tag="pgaptmp_004499"
     CDS             complement(179..1552)
                     /gene="cysG"
                     /locus_tag="ACHKNB_RS22495"
                     /old_locus_tag="pgaptmp_004499"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HGU4197882.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1571..2377)
                     /gene="nirC"
                     /locus_tag="ACHKNB_RS22500"
                     /old_locus_tag="pgaptmp_004500"
     CDS             complement(1571..2377)
                     /gene="nirC"
                     /locus_tag="ACHKNB_RS22500"
                     /old_locus_tag="pgaptmp_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HGU4197883.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2504..2830)
                     /gene="nirD"
                     /locus_tag="ACHKNB_RS22505"
                     /old_locus_tag="pgaptmp_004501"
     CDS             complement(2504..2830)
                     /gene="nirD"
                     /locus_tag="ACHKNB_RS22505"
                     /old_locus_tag="pgaptmp_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HGU4197884.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2827..5370)
                     /gene="nirB"
                     /locus_tag="ACHKNB_RS22510"
                     /old_locus_tag="pgaptmp_004502"
     CDS             complement(2827..5370)
                     /gene="nirB"
                     /locus_tag="ACHKNB_RS22510"
                     /old_locus_tag="pgaptmp_004502"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HGU4197885.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5632..6813)
                     /gene="tsgA"
                     /locus_tag="ACHKNB_RS22515"
                     /old_locus_tag="pgaptmp_004503"
     CDS             complement(5632..6813)
                     /gene="tsgA"
                     /locus_tag="ACHKNB_RS22515"
                     /old_locus_tag="pgaptmp_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HGU4197886.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAAVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7084..>7647
                     /gene="ppiA"
                     /locus_tag="ACHKNB_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /gene_synonym="rotA"
     CDS             7084..>7647
                     /gene="ppiA"
                     /locus_tag="ACHKNB_RS22520"
                     /old_locus_tag="pgaptmp_004504"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HGU4197887.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKV"
BASE COUNT         1897 a         2022 c         1950 g         1778 t
ORIGIN      
        1 taagaacgat acaggaatac ttaattggcg tcagtgagcg aatgacttaa ttttgtaagt
       61 cgctgtaacg gtgaatataa cccattagca gataatattt attaaggggc tatgttttat
      121 ttgctgaata tataaaaaga gtcattaaga aggccagaat tctctggccc gggttaaatt
      181 aatggttgga gaaccagttc agtttatcgc gcaggccaac aacccgacca ataataatta
      241 gcgatggact gttcatttgc tgagcaagtt caccgagttg cgtgagcgta ccgtcaatca
      301 cgcgctgcgt gactgctgtt ccgttttcga caattgccac cggcatttcg ccaggcatac
      361 cgtgttcaat cagcttttgc tgaatagtcg cagcctgatt cagccccata tagaacacca
      421 gcgtctgttt ttctgccgcc aggttttccc agtccagctc gccaccggtt tttaagtgtc
      481 cggtaattaa gcgcacgctc tgggcataat cgcgatgcgt gagcggaata cccgaatagg
      541 cagagcaacc agaagctgcg gtaatacccg gaaccaccga gaacggaata cccgcgttgc
      601 acagtgtttc cagctcttcg ccaccacggc caaaaataaa cggatcgcca cctttcagcc
      661 gcaccacgcg tttgcctttt tgcgcttccc gcagcaggat ctggttaatc tcttcctgcg
      721 gtacacagtg gtatcccgcg cgtttgccga cgaaaacgcg atcagcatcg cggcgtacca
      781 gattcataat atcgtcagaa accagacggt cgtagaccac cacatctgcc tgctgaattt
      841 gttgcagtcc tttcagcgtc agcagcccgg catcgcccgg acctgcacca accagcacca
      901 cttcaccgcg atggtcgagc ggttcgttga ttaactgttc ggtcgtttca gtaatggctt
      961 tctgatcgtt gtttgccagc gactgcgcca ggcggtcgtt aacgaacaat ttctcccaga
     1021 aacggcgacg ctcactcatc gtggcgaact gttgtttcac tcgcccgcgt aattgcccgg
     1081 catatttcgc cacctggccc agatgcaacg gcagcagtga ttcgagtttt tcgcgcaaca
     1141 ggcgcgccag aaccggagag gtgccgccag aggagaccgc gaccatgagc ggtgagcggt
     1201 caataatcga cggcataata aagctggcgg ctttcggcgc atcgaccacg ttacagaaga
     1261 tgcgacgagc ttcagcggct tcgctgacgc gctggttaag cgcgtcatca tccgtcgctg
     1321 caatcgccag ccagcaggtg tcgagaaggc tttcatcaaa tggcccttcg acgagggtta
     1381 acatgcctgc atctgcccat gcggtgaact gtggaataaa cgctaatgca ttcaccgtta
     1441 agcgagcgcc tgcgtctaac agcaaccttg ctttgcgttc cgcgacatca ccaccgccga
     1501 caatcagaca gtcgcgatcg cgtaattggc aaaatatagg caaatgatcc acgtaaaaac
     1561 cccttagtaa ttaaccggca gccgtttcag tttgattaaa tttgtccgca accggacgat
     1621 tcgctttcgg cgtagcatac caataaccca atcccatgaa tacggcacct gataaagtat
     1681 tacccagcgt cacccacagc aggttatgac caatacccgc cagcgtgtag gcttcgctgt
     1741 ggttgccgaa ccaggagagc gcgaacagcg tcatgttagc gatagagtgc tcgtagccag
     1801 acgcgataaa tgccagcaga caccaccaga tagcgataaa tttcgccgcc ccttcagtgc
     1861 gcagcgccat ccagattgcc aggcaaacca gccagttaca caatgcacct ttgaagaaca
     1921 gtaccattgc cggtgcagtg gttttagcca gcgcgacgga gtgaacgatg ctggtatcta
     1981 ccggcagcag gctaccgccg ccccagctat agagcatggc aacgaagacg gaaccgacca
     2041 ggttacccag ccaggtttgc ggcaggattg cccacatttg cccgtggctg atgctgcccg
     2101 cttttacccc aaaggtgagg aacatggtgt gtccggtgaa cagttcagaa ccggcgataa
     2161 tcaccagcgt taaggcgata ccaaaggtcg cgcccatcac cagcggacgt acggacgggt
     2221 cgagcaaatt accgagcgtg aaaatcaaga tgatcccaag acccacatac gcgcccgcca
     2281 tggcggagct gacccaaaag ccgagcgggt tatttgccga caggcgtgca atgcgcgcag
     2341 cgttagccgc acacttatta atagtgtctg taaacatttg attatccttt aaaaaataaa
     2401 aacaaaaaaa taaaacaaaa agtttaaaaa atgtcctgca atataccgaa ggtcattaca
     2461 ggaccatgca aaaaggggag gcattgcgcc tcccgttaaa acattaaccg cgcagctgca
     2521 ccacgccgtc tttcactcgt gcttcgtaat gtttgacgga aaactgttcg tcttccatgc
     2581 acaagccatc acttaagcga aaacgctgtt ttttcagcgg gctggcgacc cacagctcgc
     2641 cctggtgttc cgcaatcagc ccgcgtgaca gcacgctgga ctcgaagaac gggtcgatgt
     2701 tgctgatcgc aaacacctga tcgctgtgat acgggcggaa aattgctacc tgctcgtcac
     2761 ctaacagcgc gcagacgccg gtttcaggca ggatgtcatc gattttgcag atgtctttcc
     2821 actggctcat gcgttgtcct ccaccagagt taccgggata cgttcatacg gcgttgccgg
     2881 acggtgctgt tcgcgctctg gcaccatctg tacgttcggg tcacgcttgt cgctgttgat
     2941 gaagtgtttg aagcgagtct gcgcggacgg tgtattgacc gtttcagtcc actcacacac
     3001 taccgcttca cgcaggcgcg ccatctcttc ttccagatgt gcgttcagcc ccagtttgtc
     3061 gtcaatgatc accgctttca gataatcgat gccgccttcg aggttttcta accacggcgc
     3121 ggtacgcgtc agtttgtcgg cggtacggat gtagaacatc atgaagcggt cgagatattt
     3181 gatcagcgtt tcgcgatcga tatccgctgc cagcagatcc gcatgacgcg gtttcatgcc
     3241 gccgttaccg caaacataca ggttccagcc tttttcagtg gcgataatac ccacgtcttt
     3301 accctgagct tctgaacatt cacgggtaca gccggagaca ccgaacttca ttttgtgcgg
     3361 cgtacggatg cctttgtagc ggttttccag ttccacgccg aggccgacgc tgtcgccaac
     3421 gccgtagcgg caccaggtgc tacccacgca ggttttcgcc atacgtagtg ctttcgcata
     3481 ggcatgaccg gtttcgaagc cagcttcaat cagctgacgc cagatctccg gcagatcgtc
     3541 tttctgtgcg ccaaacatcg ccaggcgctg agagccggtg attttggtgt agagattaaa
     3601 ttcacgcgcg atacgaccca ctgccatcag tccttccggg gtgatttcac cgcccggaga
     3661 gcgcgggatc accgagtagg ttccgtcttt ctggatgtta gcgaggaagt tgtcgttaga
     3721 atcctgcagc ggagtatgtt ccggcttcag aatgtattcg ttccagcagg aggccagcag
     3781 cgaaccgacg gttggtttac aaacttcaca accgtagcct ttgccgtgtt tcgccagcag
     3841 ttcttcgaag gttttaatgc cttcaacgcg gatcaaatgg aacagttcct gacgcgaata
     3901 agcaaagtgt tcgcacaggt tgttgttaac ttcgatgccc tgtttcgcca gttccgcgtt
     3961 cagtacctga gtgaccagcg ggatacagcc accgcagcca gtacccgctt tggtttcagc
     4021 tttcagcgcc gcaacggtgt ggcagccttt gttgatggca gcaatcagat cacctttggt
     4081 gacgtcgaag caggagcaga tttgcgcgct gtccggcagt ttatcaacac cgatagacgg
     4141 cttgccgcta ccagagtgtg ctggcaggat cagggaatcc gggttttccg gcagttcgat
     4201 agcgttcagc accagttgca gcaggttacc gtagtcgctg gtatcgccca ccagtaccgc
     4261 accgagcagg gttttgttgt cttcgctgac aatcaggcgt ttgtagatct ctttactttc
     4321 gtcgaggtaa acgtagctac gtgcgccagg cgtgcgaccg tgcgcatcac caataccgcc
     4381 tacgtctacg cccagcagtt tcagcttggc gctaaggtca gcaccttcaa aggcgttttc
     4441 gctaccgaga atatggtcaa cggcgacctg cgccattttg tagcctggtg ctaccagacc
     4501 aaatacacgg ttgttccagc ttgcgcattc accgatggcg tagatatccg gatcggaagt
     4561 ctggcaggaa tcattaatga caataccccc acgcggagca acgtccagac cacactgggt
     4621 tgccagctta tcgcgcggac ggataccggt agagaagacg ataaagtcga cttccagttc
     4681 gctgccgtcg gcaaaacgca tggttttacg cgcttcgaca ccttcctgca caatctcaag
     4741 ggtgtttttg ctggtgtgaa cgcgcacgcc catactttcg attttgcgac gcagctgctc
     4801 gccacccatc tgatcaagct gttccgccat cagcataggg gcaaattcga taacgtgggt
     4861 ttcaatacct aagtttttca gcgcgccagc ggcttccaga cctaacaggc cgccaccaac
     4921 aacggcaccg cgcttgctgc gacgggcgca ggattcaatg gcgttgaggt cttcaatggt
     4981 gcgatagaca aagcagtcct gagtatcaga acctttgatt ggcgggatcc acgggtagga
     5041 accggttgcc atgatcagct tgtcataaaa aacggtacgt ccggcgctgg agtgaatcac
     5101 cttctcctga cggttgatgg tgatagcgcg ttcgccgacc agaactttga tgccgtgttt
     5161 ctcgtagaag ccttcgcgca ccagcgacag ctcttcggcg gtgtggtgag agaagtaaga
     5221 cgagaggtgt acgcggtcat aagcgatgcg cggttcttca cagaaaacgg taatatcaaa
     5281 gttggccgca tcagatttat caagaagatc ttcgataaag cgatggccga ccataccgtt
     5341 accgataatt gcgagtctga ctttgctcat ttttgcctcg atttcttttc tattaccgcc
     5401 taccttaacg attcagcaac cccgcttatt gatgtaaatc aaattcacct ttatatactc
     5461 cttaaggggt atattgctga tttgtataaa ttttcttaag tcacggaatt gtctgatatt
     5521 tcatgtttgt gcaaaaaata gacaaaatga acgggtttat agaaatagat gcacgtagcg
     5581 ggggaagaga ggaaaaggcc ggacgacgat agtgccgtcc ggcattagca attaatgaga
     5641 ggtcagggtg ttatgctgac ggtgacggct gacgaaacct aacaggaagc acatcacaaa
     5701 gacggcagcg tacagaccgt ttgctgtaag cagtgccgcc tgcggaccgc tatgttcaac
     5761 aatcgggccg gtaaccacaa aggtcaacat agtaccgatg gttccgcagg tcaggacaaa
     5821 gttaaccagt tttggcgacg gtactttggt ctgctgtgaa cccagggtga tgatggtggt
     5881 atagatcgcg ctggagaaga agcccagagc gagaattgac cacgccatat gtgctggtgt
     5941 tccggtgtta aagacgtaca tcagaatcgc agccagacca gccagtacgg tcagaatgcg
     6001 ttgcaaatca aagaagcgaa gaataaagct gaacgcccac atgccgacca tgtatgacat
     6061 ccagaagtta ctcaccagcg tgcccgcgtc gttcaggctc atgcccaggc ctttggcata
     6121 ctcaggcacc caggagataa aacctaactg accgaggatg tagcacagcg ccgcaacaga
     6181 gagaaacagt acgccgatcc cccacttttc tttctctacc ggagcatccg tttttggcgc
     6241 atgtttgccc agcgccggga actcacagcc gaaggtcaga ataaaaatag cgacatacac
     6301 cagcccgatg caggcataaa cccagtacca ctcaatgctg cgcgccagta gaaacgcggc
     6361 gatcattggg aaaatcatcc cagccatact gaagaaggag tcggtaaata acaggcggga
     6421 accgcgctga cgcccttcat acatttgtgt taccaggaat gtaccaatcg acatggtgat
     6481 gccgctgacc accccgagaa tgaacatcgc cgccgagaac agcgccaggc tgtggctgaa
     6541 catcaaaccg gcaaccgcca gcaccatcag gagaaagcca aaacgtaact gcgttttcaa
     6601 cgggacgatt tccatcagcc aggcgttgag gaagatagag attaaaatgc cggcgttgag
     6661 gaaggtgaag gtgttactca tactggaaac aggcagattg aaataatcgg cgatatttcc
     6721 catcaccatc ccggtgacaa taaccaacgc accagtcagt gcgtaggaga gaaagctaat
     6781 ccatgtgagc ttgatgcgat tgctgttagt catgattggc ctgcgttcaa aaataaaatg
     6841 gcatagcggg atatgccgcg agcgggcgat tttaggtgat tttgtgatct gtttaaatgt
     6901 tttattgcaa tcggttgcta aattgcattt taagaggtga ttttgatcac ggaataaaaa
     6961 gtgatcgtca ggttacatat atttcagata cgtaaaatta ggtaaaggga tggccttgtt
     7021 cttgaaggct atttagaatc tcttcacttg ctttttttct gctctgtttg ttaaggaaat
     7081 ctcatgttca aatcgaccct ggcggcgatg gctgctgttt tcgctctttc tgctctttct
     7141 cccgcagcaa tggcagcgaa aggggacccg cacgtattgt tgacaacctc agctggtaac
     7201 atcgaactgg agctggataa acaaaaagcg ccagtgtctg tgcaaaactt tgtcgattat
     7261 gtgaacagcg gtttttataa caacactacc tttcaccgcg tcattcctgg ctttatgatt
     7321 cagggcggcg gtttcaccga gcagatgcag cagaaaaaac caaacccgcc aatcaaaaat
     7381 gaagccgata acggcctgcg caacacgcgt ggcaccatcg cgatggcgcg taccgctgac
     7441 aaagacagcg ccaccagcca gttctttatc aacgttgccg ataacgcctt ccttgaccat
     7501 ggtcagcgtg atttcggtta cgcggtattt ggtaaagtgg tgaaaggcat ggacgttgcc
     7561 gataagattt cccaggtgcc gactcatgac gttggtccgt accagaatgt gccgtcaaaa
     7621 ccggtagtta tcctttccgc taaagtc
//
LOCUS       DAZFVO020000203         7636 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFVO020000203 DAZFVO020000000
VERSION     DAZFVO020000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.204"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            209..1372
                     /locus_tag="ACHKNB_RS22525"
                     /old_locus_tag="pgaptmp_004505"
     CDS             209..1372
                     /locus_tag="ACHKNB_RS22525"
                     /old_locus_tag="pgaptmp_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HGU4197888.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIEVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ESELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1377..3524
                     /locus_tag="ACHKNB_RS22530"
                     /old_locus_tag="pgaptmp_004506"
     CDS             1377..3524
                     /locus_tag="ACHKNB_RS22530"
                     /old_locus_tag="pgaptmp_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HGU4197889.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3584..4831
                     /locus_tag="ACHKNB_RS22535"
                     /old_locus_tag="pgaptmp_004507"
     CDS             3584..4831
                     /locus_tag="ACHKNB_RS22535"
                     /old_locus_tag="pgaptmp_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HGU4197890.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYARTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5117..7060
                     /locus_tag="ACHKNB_RS22540"
                     /old_locus_tag="pgaptmp_004508"
     CDS             5117..7060
                     /locus_tag="ACHKNB_RS22540"
                     /old_locus_tag="pgaptmp_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197891.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPVLENFTAGQSGTLVEGINNGIIWGGIAFGAGLAAIALAAGGGGGSSRHKRATEDE
                     QKPVQPEGGQEAGKTDVSSTGSIEPESGNLSGRVTDQDGNGKADAEEAAAAVAAATEK
                     VEAAEAADKAAKDKLAELNADKLITPEEKAQLETAKQNADTLKGEADSAVKALPDTAA
                     EKGGLQGRVDALDGIQVPEVNDQDGNGKADAEEAAVAFYERALPSNVSGDLYPQPSIF
                     GDKVSSVSKNWSTLLDSNPGSYVTSQRLDSGANQYNYNGHTGSDVISITDSFGGLDRT
                     QVSRFPVGLFTGEGNDLIVTGRDYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVT
                     LYVNEEGQLRATTDSYNGSTSIDYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGN
                     SADAIINTNIDLGAGNDFIYANGEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSG
                     FEHVDLHSTSHLILNSDDFKSQDIEGEILKISGSSGASVDVQNFDWENLSSANDGDVK
                     YFTYQSTDIPGLTLWIQEGIEVK"
     gene            7119..>7636
                     /locus_tag="ACHKNB_RS22545"
                     /old_locus_tag="pgaptmp_004509"
     CDS             7119..>7636
                     /locus_tag="ACHKNB_RS22545"
                     /old_locus_tag="pgaptmp_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197892.1"
                     /translation="MGLFNYKDYHQAQITGLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSL"
BASE COUNT         2003 a         1553 c         2149 g         1931 t
ORIGIN      
        1 gcttggatca attccggtgt ctctgcattt caattttact caatatttta cccggttgca
       61 tcatgatgcc tgattatatt tcccgcgtgt cccggaggtt cgggtgaaga gaatattatc
      121 tttctgtttt cttttctctg tccacaacgt gttcgcttct ccactgccgg ccctcctgag
      181 ggatgctctc attcatgatc catcagtgat ggaggcaagg gcagaagcca cctctgcccg
      241 ttcccgtgtg gagcaggctc gttcagcaca ctggccggtc gtcacagcaa caggcagtaa
      301 actcctttcc cagagtcacc gctacgacta tgactacgac tctgaagata ttttgcccgg
      361 tatccgcggt gaaatgaatc tgtttgcttc gggagctatt gaggcggatg tcagccggag
      421 tgagtcggaa gccgagtatt accgttataa agttgatgag gcaggcgagg aaacaattcg
      481 ctcctttgtt tcattatatc ttgatgcgct aagggaaaag caatccattg aggtacttga
      541 acagagtctc tcccggcata acgcaattct taatgatctt aacaccatca gtattcacga
      601 caccgggcgg gagtctgagt ttgttcaggc cgaagcccgc aggctgatgg tccggcagca
      661 gataaatacc cgcacccgag tgctcaaaac cacgctggga aaactttcga cctggaccac
      721 aaatccggta accgaagccg atcttgaaaa tcctttttcc cggatgacag agtccagatt
      781 gttaactgat tttacgcaga cgcctcagaa agggaatccg tcgtggcttg ccagtcaggc
      841 tgatgtagag agtaaaaaag cggcgctgaa agcgcaggag cttgcccggt atccccgggt
      901 ggatttaacg ggatcagtga cccgggatga caggcagata ggtgtcagtc tgtcctggga
      961 cctgttcaac cgtaatgcca gttatggtgt cagtgaaaag gccgcgcaga tagctgcagc
     1021 taccggacga ctggactctg ttgcccgaat gattgatgaa accggacgat tatcgctgat
     1081 aacggtcaga caaagtcgga tggaaatgga aacgctgaga cgtcaggagc aggcctctgc
     1141 cagagttgtg gacttttatc gtcttcagtt cagggtggca agaaagacac tgattgaatt
     1201 actgaatgca gaaagcgaac tgtacagtgt cgggctttcc cgggttcaga cggaggatca
     1261 gatgctccac ggaatgctgg attatctgta ttcccaggga atgctcctga aatggagcgg
     1321 agtgaatctt tctggtgcag aagaaaaagt aacagggcag aaggacagat aaataagtga
     1381 agagtatttt tgaaggatta tcacaggtaa cggcattact gggtacacct gtatctgctg
     1441 agacgctggc tgccggtaca gtccggacag atgtctccgg gattgatttt cgttctgttg
     1501 gcgagtttct tcgcagtgaa gggtttgata atcatctttc ccggcgggct ccggaagata
     1561 ttccttcact tgcggttcct gtgcttcttt tactgaatgc acaggaagcc attgtggtgg
     1621 tgcgtattga gggtgccggc ctggcccggc agtatcatgt actccagccc gggggactcc
     1681 gtaagacact ttctcacgat gaattaatgg ctgtttattc aggttactgc tggtttgttc
     1741 agccacaggc aacacaggat cgccgttcgg aactgccgga gtatgacatg accggggcat
     1801 ggttctggcg ggtactgtgg cggttcaggc catattacgg acaggtcatt cttgcgagtt
     1861 ttatcattaa ctttcttgcg ctggtcagtt ccctgtatgt catgaacgtt tatgaccggg
     1921 tcatacccaa tcgctcctgg gaaacgttat ggatgctgag tatcggggtg attgcagcca
     1981 ttctgtttga gtgtgctgca aagttaatcc ggagctatct gacggatatt gcgggtaaga
     2041 aggcagacct gattatcagc tcagcactgt taaggcgggt gatggcagtg aaaatgtcgg
     2101 acaggccggc gtcttccggt tcatacgcca gcaatttgcg ggaatatgag tctgtcaggg
     2161 agtttatgac cagcgccacg ctgctggctt ttgtggattt gccgtttatc ctgcttttca
     2221 tcggtgtcat cgggcttgtg gggggctggc ttgctgttgt tcctcttaca ctggttccgc
     2281 tggtgattct ggcaggaata ttactgcagc gaccgctttc gcgtcatatt aatgcctcca
     2341 tgcgggaatc atcgcagcgc cagggattac ttgtggaggc tctggacgga atagaaaccc
     2401 tgaagctgaa taatgcagtt aactgggtcc agcgccgctg ggatggatat acagccagtg
     2461 tcgcccgctc gtccatacgg gtaaaaaata ccagtaatgt cataatctgc ttttccacag
     2521 cagttcagca actgaatact gttctgctgg tcattctggg gacgtacctt attcacagtg
     2581 atgacccaca taaccgaatc accatggggg cactgattgc cgcagttatt ctttccggtc
     2641 gtgcgctggc accgcttggt cagatagcat cactggccac ccgttttcag caggcccgcc
     2701 tggccatgca gggggtgaat gacattgtcc gccgtccggt ggagcgggaa agtcagacac
     2761 attatctgac agtgaaggat atccagggag agtttcgttt cagtgatgtc tccttttctt
     2821 ataagcccgg attacccgac gcattgtgcg gtctgtcgct ctgtatccgg cccggagagc
     2881 gggtgggtat tattggctca ataggttgtg gtaaatcgac gctgctgaaa atgatggtgg
     2941 ggctgtaccg tcagaacagg ggacaaatta ctcttgacgg ggttgcgatg cagcaactgg
     3001 acccctgttt tgtccgggat aaagtgtttc ttctggaaca gaatccccgg ctttttctgg
     3061 gaactctgcg tgaaaatctg cagctttcgc gtctgccggg aagcctgtcg gatgtggata
     3121 tgattaatgc agccggacgt ttcgggtttg accgttttat ccggcatcat ccccgcggac
     3181 ttgatatgca gacaggggaa ggcggtcagg gactgtcagg aggacagagg cagatggttg
     3241 ccctgacgag aatggtactg ggttctcccc gggtggtact gcttgatgag cccacgacag
     3301 gtctggacct gcagactgaa tctgttgttc tgaaaggtct gagccactgg agccggggac
     3361 gcacaatggt ggtggtgaca catcgtctgc aggttctgaa tattgttgac cgtattgtcg
     3421 tgatgaatga agggcgggta atgatggacg gtccccgtga tgaaatactg aaaaaactgt
     3481 cagggaaaca gcagagtggg gagagcagag cgataacttc atgagcgtca accggataag
     3541 aaaaaagagc agatatgttt aacagtttgt ggaccgcagg gatatgacag aaaaaacgga
     3601 acggaataaa gcgtcgtcag aggtggtcag gcagtcagac ctggcgctgt tgacagatgt
     3661 acaggcagcc cttcagcagg aaaagcatca tgcccttttc ctgatggtga tttttctgct
     3721 ggtactggta gtgacgtttg ttatctgggc atggaacagt cctctggatg aagtgacccg
     3781 aggtcagggg agtattattc ccggcagcag ggagcaggtt atacagaccc ttgacccggg
     3841 tatactgaaa tcacttgagg tcagggaagg cgatattgtt gagaaaggtc aggtactgct
     3901 gacactggat gatacccgga gctctgccat gctgagggaa agtgaagccc gggtgaataa
     3961 cctggaggcc gtcagggcaa gactccgtgc agaagcttat tccgaatcac tgacatttcc
     4021 ggataacgtt cctgaggatt tgcgggagcg ggaaagtacg gtctattttc tccggaagac
     4081 ggagctggcg cagagcattg ccggtctgaa gcagagtaag gcgctgctgg acagggaaat
     4141 agcaatgacg cggccaattg tgcgtgaagg ggcaatgtcg gaggtcgaac tgttacgtat
     4201 gcagcgtcag tcagcggagc tgcagttaca gatggatgag aaacagaata aatacctgac
     4261 ggaagccggt gctgaactgg tgaagacaga agcagagctt gctcaggcaa aagagaatat
     4321 ggctggcaga gccgacccgg ttgagcgttc acgtctcagg gcccccctga gaggtatcgt
     4381 aaaaaatata cgggtaaaca cactgggagg ggtggtttct gcaggacagg acataatgga
     4441 aattattccg ctggaagacc agttgctgat tgaagcttat ataaatccac gggatgttgc
     4501 ctatgccaga acagggatgc cggcacttgt aaaactgacg gcttatgact atgccattta
     4561 tggtggtctt gacggcgtgg tgacactggt aagtccggat actttacgtg accagaaacg
     4621 gccgggagat ttaaaactgg acccgaatga agcttattac cgggtactgg ttaccaccag
     4681 taataattat ctgacagaca ggaacggaaa aagattgcca gttattcctg gcatgatagc
     4741 cagcgtggat ataaaaaccg ggcgtaaaac ggtatttcag tatctgatta aaccgataac
     4801 cagaatgaag caggcacttc aggaaagata ataaaaaggt cttccgggga aagtatttta
     4861 aatcaatttt aaacgggcag aatgatttca ctttattctg cccgggtcat tgcgcatgcc
     4921 tgtatgtgat ttggggctga agataatata aatgttatat ctgtcagggg aaaataaatc
     4981 tgaaaaaagc ggaatgatta aggaggtccg gtcagagaaa taaattatct gtaatctgat
     5041 tcgtttttat ttaatgactc cctttagcgg ataaaacata agagcatact ggttttatta
     5101 acctgaggat acttatatga aaatgatttt caccggcaag gtcagtggag aaaaaacagt
     5161 actgacagcc ggagcccgtc atacagtaaa ggcacaggcc ggggaacagt atggactggt
     5221 tgatgaggtg acaggtctgg tcccggatgg tgtggaagcg gaccgttctg gtgatgattt
     5281 aattctgcgt aaaaaggaag atgatacaga aatccggatt gagggattct gggaagaatg
     5341 ccagccgggt gaaacacagt gtacagcagt atttaatgtt gtgggtgaaa atggtcaggt
     5401 aactgaagct gtcctgaccc aggatggccc tgtactggag aatttcactg cagggcaaag
     5461 tggcacactg gttgaaggta tcaataacgg gattatctgg ggggggattg cttttggtgc
     5521 agggctggcg gctattgcac tggctgccgg aggcggtggt ggaagcagtc gtcataagcg
     5581 tgcaactgaa gatgagcaga agccggttca gcctgaaggc ggtcaggagg ctggaaaaac
     5641 agatgtatca tccacaggct caattgagcc ggaaagtgga aacctctctg gacgcgtgac
     5701 ggatcaggac ggcaacggca aggcggatgc agaggaagcg gcagcagcgg tggcggcggc
     5761 cacggagaaa gtggaagcgg cagaagcggc ggacaaggct gcgaaggaca agcttgcaga
     5821 gctgaatgca gacaagctga tcacgccgga agagaaggcg cagctggaaa cggcgaagca
     5881 gaatgctgac accctgaagg gtgaggcaga cagtgccgtg aaggcgttgc cggatacagc
     5941 cgcggagaaa ggtggcctgc agggtcgtgt ggatgcactg gatggtatcc aggtaccgga
     6001 agtgaacgat caggacggca acggcaaggc ggatgcagag gaagcggcag tggcatttta
     6061 cgaacgggca ttgccctcta atgtatcagg agacttatat cctcaaccgt ccatatttgg
     6121 tgataaggta tcttctgttt ctaagaactg gtctacttta cttgatagta atcctggaag
     6181 ttatgttaca tcccagagat tagattcagg agctaatcaa tataattaca atggtcatac
     6241 aggttctgat gttatctcaa ttaccgattc tttcggagga ttagatagaa ctcaagtatc
     6301 tcgcttcccg gttggtttat tcacaggaga agggaatgat ttaatcgtta caggaagaga
     6361 ttatggacgt aataccagtg caggatacac ggatcactct catcgtactg atatggggaa
     6421 tggagatgat accttggttg ttggtgtcgg aaataacgat gtaacactat atgtgaatga
     6481 agaaggacaa ctgagagcaa ctactgatag ttataatggt agtacatcca ttgactatac
     6541 tgggctcaat tcttcatcat caggtggaac aatatcagga actgatattg ttatgggggc
     6601 aggaaatgat actgttctgg ctttagggta tgaaggtaat agtgcggatg ctattattaa
     6661 tacgaatata gacttggggg caggtaatga cttcatttac gctaatggag agatttcaac
     6721 taacaatggt actcaggtaa atattatagg tggagagggc tttgacacga tatctttgga
     6781 caatactaca gtaacaagtg ctatgttctc tggttttgag catgttgatt tgcactctac
     6841 atctcatctc attctgaatt cagatgattt taaatctcag gatatagagg gggagatatt
     6901 aaaaatctca ggtagttcag gtgcatccgt ggatgtacaa aacttcgact gggagaattt
     6961 aagtagtgcc aatgatggtg atgttaaata tttcacctat cagtccactg atattcctgg
     7021 tttaactctt tggatacagg agggtattga ggttaaataa aagaaaatct aaacaaacag
     7081 ccccagcatg gggctgttct ttaaataagg aaatactcat gggactcttt aattataaag
     7141 attatcatca agcgcaaata acaggcctga tctctgcgtc ttataatatt gcagtatatg
     7201 ccaatacagg aaaggttctt ggattaggaa gttttctggt atccaggcta tttgcaaagc
     7261 acccagatag gattgattat tctaccgatg ttcttaatga aggctggcgt gatattacag
     7321 tggaagaact caggttatct tcggaatatg tcgattcaag aggtttttat cgctttccca
     7381 gtccggttac aggaaaccct gcgtcaggat ttggtcctca gttgaaagtt gttgggttgt
     7441 ttgatgctca acagaatcta cagaaaattg caatatcatt ctgtggaaca aatgatatct
     7501 gcgatattgt tgattataca cagctcaata atggaaaagc gctgtctgaa gcagaaaacc
     7561 tgcttcaaag tatcagagac tttgcaatca gtaatggttt aagtggtgaa gatgttattg
     7621 tcacaggcta tagcct
//
LOCUS       DAZFVO020000204         7566 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFVO020000204 DAZFVO020000000
VERSION     DAZFVO020000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.205"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..568
                     /locus_tag="ACHKNB_RS22550"
                     /old_locus_tag="pgaptmp_004510"
     CDS             <1..568
                     /locus_tag="ACHKNB_RS22550"
                     /old_locus_tag="pgaptmp_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4197893.1"
                     /translation="AINRNTRGLLSQRDLLETHEERLNSQQQQINTGSTVAVDSHGYV
                     TRGEGTGERITVQEGLVRTQEMATENRAAVSRNRQVGERNSRAIASQARTLQAHERRM
                     NSQQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFNATAAVGGYSDRQAVAVGMGYR
                     FNEQAAAKAGTAFSDGDVSWNVGVNWEF"
     gene            696..902
                     /locus_tag="ACHKNB_RS22555"
                     /old_locus_tag="pgaptmp_004511"
     CDS             696..902
                     /locus_tag="ACHKNB_RS22555"
                     /old_locus_tag="pgaptmp_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197894.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            965..1441
                     /locus_tag="ACHKNB_RS22560"
                     /old_locus_tag="pgaptmp_004512"
     CDS             965..1441
                     /locus_tag="ACHKNB_RS22560"
                     /old_locus_tag="pgaptmp_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4197895.1"
                     /translation="MQIIIMTRDRYLEYGLMCMLSGYRLTTGRELFDAGKRRLPLPED
                     SYVILCDCNLERLTYCMFCGRRFLVIPVSSVRCLTDIRQAIRRGAWLFGHTARPLTRT
                     EMVVVFGVVFHEYAFTFLADQLGISMKTVCAHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            1537..2070
                     /locus_tag="ACHKNB_RS22565"
                     /old_locus_tag="pgaptmp_004513"
     CDS             1537..2070
                     /locus_tag="ACHKNB_RS22565"
                     /old_locus_tag="pgaptmp_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4197896.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDMN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARHLGMSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKREVVK
                     QDSLFPVMLNAASDSLI"
     gene            2089..2277
                     /locus_tag="ACHKNB_RS22570"
                     /old_locus_tag="pgaptmp_004514"
     CDS             2089..2277
                     /locus_tag="ACHKNB_RS22570"
                     /old_locus_tag="pgaptmp_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HGU4197897.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            2421..3269
                     /gene="mscS"
                     /locus_tag="ACHKNB_RS22575"
                     /old_locus_tag="pgaptmp_004515"
     CDS             2421..3269
                     /gene="mscS"
                     /locus_tag="ACHKNB_RS22575"
                     /old_locus_tag="pgaptmp_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HGU4197898.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIIGGIAARMISGVLNNLMLGRN
                     IDATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNL
                     AAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIV
                     NFSREPERRNEFIISISYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMN
                     FVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSR
                     SVSSRQ"
     gene            3395..4615
                     /locus_tag="ACHKNB_RS22580"
                     /old_locus_tag="pgaptmp_004516"
     CDS             3395..4615
                     /locus_tag="ACHKNB_RS22580"
                     /old_locus_tag="pgaptmp_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HGU4197899.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            4845..6590
                     /locus_tag="ACHKNB_RS22585"
                     /old_locus_tag="pgaptmp_004517"
     CDS             4845..6590
                     /locus_tag="ACHKNB_RS22585"
                     /old_locus_tag="pgaptmp_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGU4197900.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(6772..7401)
                     /locus_tag="ACHKNB_RS22590"
                     /old_locus_tag="pgaptmp_004518"
     CDS             complement(6772..7401)
                     /locus_tag="ACHKNB_RS22590"
                     /old_locus_tag="pgaptmp_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HGU4197901.1"
                     /translation="MSIEQIINEINVYLNSLKKEDERIDAINKIKIELHRSSPFYAEP
                     VDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQPVVTLAQKGKYTVIDGF
                     HRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIRHNRARGKHQIDALSDIV
                     RDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEENFSPAWTIK"
BASE COUNT         1876 a         1461 c         2020 g         2209 t
ORIGIN      
        1 ggccataaac cgtaacaccc ggggactgct ttcccagaga gacctgctgg agacgcatga
       61 agagcgcctg aacagccagc aacagcagat aaataccgga agtaccgttg ctgtggacag
      121 tcatggttat gtgacgcgcg gtgagggaac cggtgaacga attactgtac aggaagggct
      181 ggtacgtaca caggaaatgg ccacagaaaa ccgtgctgcg gtcagccgta accgtcaggt
      241 gggtgaacgc aacagtcgcg ccattgcttc tcaggcacgt accctgcagg cgcatgagcg
      301 tcgtatgaac agtcagcagc gtcagattaa tgagaatcat gaggaaatga agcgggcagc
      361 agcccagtca gctgcgctgg ccggtctttt ccagccatac agtgtgggta aatttaacgc
      421 cacagcggct gtgggcgggt acagtgacag gcaggctgtt gcggtgggca tgggatatcg
      481 ctttaacgag caggctgcgg caaaggcagg tactgcgttc agtgacggtg atgtttcctg
      541 gaatgtgggt gtcaactggg agttctgaac ccgggtctga gtcaccgggg cgggcagcgt
      601 tctgtatgcg gtcatgattc gctgcctgct tttctccccg gtggcatgac tgacggttcc
      661 ggggtattcc tgaggagctg agagggagag tgtatatgct gaactatcgc ataagttcag
      721 tgcaggtatt tactgtaaac ggtcgggctg acagggtgca ggttcgtctg gaatgcgacg
      781 atgcagctgt ttttgaatgt tgtcttcttg cggaaggaga aggggaactg aaagagctga
      841 gtctgtcaga gctggaagag cgggcactga tgtatgcggc agacagtttc cgttatgaat
      901 gatgagtcag ttataccggt aatggtaaac ggagccggta tccgggagat aagggtctga
      961 gagtatgcag attattatta tgacccgtga cagatatctg gaatatggcc tgatgtgtat
     1021 gctgagcgga tacaggctca cgacaggccg tgaactgttt gatgccggaa agcgccgtct
     1081 gccacttccc gaagacagtt atgtgattct ctgtgactgt aatctggaaa ggcttacata
     1141 ctgtatgttc tgtgggcgtc gttttcttgt cattcctgtt tcctctgtga gatgcctgac
     1201 agatatcagg caggccatcc gtcgtggggc gtggctgttc ggacatacgg caagaccact
     1261 gacccgtacc gagatggtgg tggtcttcgg agttgttttc catgagtacg cttttacctt
     1321 tctggcagac cagctgggga taagcatgaa gacggtatgt gcgcatcttt acaatgcgat
     1381 ggagaaaaat ggtctgcgcg gcgtcagtat taaatatctc tgcagcaccg cagaccggta
     1441 aatccgggat aattaatgtt atgtggccag gcgtgataaa tttcatgtgg aacgggtatg
     1501 cattttattt gtgataacag ttattttctg gagggagtga aggagtatat taatcccggt
     1561 aaattcatta tcagcggcat gcagacagat aatgtggtga taatatcttc ttcccggata
     1621 tctgaaattg tcggttttct gtataagaat aatataagtg atatgaacac cctttatttt
     1681 tcttctccgg aagcgctccg ttttctctgg ggagtggttg acagtccggt ttatgatatc
     1741 agacatctta ctgagcgatg ttcagtaaac gatatcagtc atctgtaccg cgcattcatg
     1801 tcggtggaga agctgaccct gtcaggccgg caggtaaatg tactgatgat gctgctttat
     1861 ggcagtaacg gaacagaggg ggcacgacat cttggcatgt cagagaaaac attctccact
     1921 cataagcaga atctgataaa gaggttacgg gtacataatg aagttgagtt gttgtacaag
     1981 gggatgcttc tggtcagaaa aagggaggtt gtgaagcagg acagcttatt tccggtaatg
     2041 ttaaacgcag cctctgattc gttaatttaa taaacataag gagtttttat gcgtctgttg
     2101 attgcttttc ttcttccctg gctgactttt ttcaccattg gccggcctct gacggggatt
     2161 ttttgtctgg tgttacagat aacgctgctg ggctggatac cggcaacaat atgggccgtg
     2221 tatgcgcttt cgcagtataa gacggaacag aaaatccgtg aagccacggg gaaatgatga
     2281 ctgcagaaga gcgggttgtc atagctgtgt atatgatgcg ggataaaggc ccggagattc
     2341 ctggaaataa aatgttccgg ggaatataaa acagggaaaa accggatgtg atggacgctt
     2401 ctggcgaggc ggtttcatga attgtccgta atcaggatat gttgcccggt tacgccgcga
     2461 atcttgtggc agcagtggtt attattatta tcgggggtat cgctgcacgg atgatttctg
     2521 gtgtgctgaa taatctgatg ctgggcagga atattgatgc cacagtggcg ctgttccttt
     2581 ccgcgctgat acgtcatgcc attatagcct ttacgctgat tgctgccctg ggacgtgtgg
     2641 gggttcagac agcttccgta attgcagtcc tgggggctgc aggtcttgct gttggacttg
     2701 ccctgcaggg gtcattgtca aaccttgcag ctggtattct tctcgtgatg ttccgtcctt
     2761 tcagggctgg tgaatatgct gacctgggtg gtgttgccgg gactgtgcag aatgtacata
     2821 tattctcaac cacgatgcgt acactggatg gtaaaattgt tgtcattccg aacgggaaga
     2881 ttattgccgg agagattgtt aatttctcca gggagcctga acgacgaaat gagtttatca
     2941 tcagtatttc atatgatgca gatattgact gtgtgaaaca agtcctgaca gatattgtga
     3001 tgtcagaaga gagggttctg aagaacaggg aaatcactgt tcgcctgaat gagcctgggg
     3061 agtcatccat gaattttgta gtacgggtat ggagcaggcg tgatgacctt cagtctgtat
     3121 actgggatgt actggagaga attaagcggg agtttgattc agaagggata agttttccat
     3181 atccacaaat ggatatccat cttgttcgct ccgggaagca ggcagagtcg gtaacccata
     3241 acagtcgctc agtcagttcc agacagtaaa atgaaaagcg cattttattt gtgaaagatg
     3301 aacagcctga atctgtcagg ctgttttcat tgtgttttta tggtcatttt tggtatatgt
     3361 actgtatata atgtcatctg tacaaggata taaaatgcca aagaaaataa tgacagggat
     3421 tgatgttttt gacgctgcca tggagcgtat tgaatgggtg tttgatacgt tttcatcagt
     3481 ttgtctttct ttttccggcg gaaaagactc cactgtcctg tttcacctga tggcgaatat
     3541 tgcccgccga aaaaaacgcc gtttttctgt actgtttgtt gactgggagg ctcagtatca
     3601 gtgtaccatt gagcatgtca ccaggttgag ggaaatgtac gatgatatca cagatacttt
     3661 tttctgggtg gcgctgcctc tgacgaccgt taatggagtg tctcaggtac agcctgagtg
     3721 ggtatgttgg gaggacgggg ctgaatgggt tcgccagcca ccagaagatg ccataaccag
     3781 agcgtctttt tttccttttt atcagaatgc gatgacattt gaagagttta ttccggcatt
     3841 taatcattgg tttgcccggg aaaaagggat ggttgctgcg attcttactg gtgttcgcgc
     3901 tgatgagtca ctgaatcgtt ttatgggact ggtatccgga cgtaagctcc gttatgcgga
     3961 ggataaaccc tggaccacgg catcggctga gggattttac tacaccatgt atcctttgta
     4021 cgactggaaa acaagagatg tatggattta ccatgcgaga acacgtgctg cctataactc
     4081 tctttatgac ctgatgtaca gggctggagt tcctcttcgc aatatgcggg tgtgtgagcc
     4141 ttttggaccg gagcaacgta aggggttgtg gctttatcat gtactggagc cggatacatg
     4201 gggcagaatg tgtgctcgcg tatcaggtgc tgtaggggga gcgctctatg caaatgaaag
     4261 tggtgcttat tttgctttga ggaaacagat aacaaaacca gttcaccata catggcagag
     4321 ttatgcaatg tttctgctgg agtcaatgcc tgagaaaacg gcggagcatt acaagaataa
     4381 gattgctgtt tatcttcggt ggtggcagtc tcatggatgg ccggatggca tccctgatga
     4441 gcaggataat gaccagggat gtaaagatat tccctcctgg cgtcgcatct gtaagacgtt
     4501 actgaaaaat gatttctggt gtcggatgct ggcgtttagt ccgaataagc cccgacatta
     4561 tgagcgctac ctggccagaa tgcgtgaaaa aaggaaagag tggggaatat tgtaaattat
     4621 atttaaacat cagaaatatg acggttaatc caggatagta gctaaaaaaa taagattcct
     4681 tgctgattta tccctgtttg atatgtaaaa tctgaggggc ttataagcaa cggatatacg
     4741 agtgctacaa tatatgataa gttatatata ttaaatgttc atcatctgtc aggggctgtt
     4801 ttatggctaa actgataaat atatcaggtg ttaccgagga gcggatgact gccggggaaa
     4861 agcgggttgc atcccgtctt gaggcattta tgagtaatga gtgtctggtc tggtacgata
     4921 ttccggtggg acggaaaaac cgacatcctg attttgtcat tattgacccg gagaatggtc
     4981 tggtatttct tgaagtcaag gactggaccg ttcacacgtt gcatcaggta aatcacgaac
     5041 aggtcattct tgaaactaac ggtattctta aatcagaaat aaatccgctg ctacaggttc
     5101 gtcgttatgc ctgtgatacc gtaaatgctt tacctgcaga ctcacgtttg cgacagaatg
     5161 atggtcagta taaggggcgt ctgaatctgg cctgggcgta tggtgttgtg tttacccgta
     5221 ttacccgcca gcaactgaag gctctggctg ggaatgatga aaatgctgtg gaaaggattt
     5281 tcccttcagc gcagacaatc tgtcaggatg atatgactca gtctgtttta cctgatatat
     5341 tccgccaaaa aattgccggg atgtttacga tgggattcag aacccgggtg acaccacagg
     5401 ttagagatat tctgcgtgct catctgtttc ctgaaatgac cgtcagacag cactcacaaa
     5461 tcaaaataat ggatattcag caggagattc tggccagaaa tattggtgat gggcatcgtg
     5521 ttattcatgg ggtagccggc tctggaaaaa caatgattct tttgttccgt tgtctttact
     5581 tggctgaaac tacccctggt aagattctgg tgctatgtta caacattact ctggccagct
     5641 acctgcggga atgtattgag tccaggggac tgaagtcacg ggttacggta ttccattttc
     5701 atagttggtg cgcctcaatg ataaagcgcc atggaattca ggtgactgct ggaggaaagg
     5761 attacccgga gaaatgcttc agtgcgctgg aagatgccgt gaacagcgga acaattactg
     5821 atactggata cgatgccgta cttgtggatg aggggcatga ttttgagagt cgctggctgg
     5881 ctctgatagc ccgcctgttt gataacgcgt cccgttcact gctcctgatg tacgatgacg
     5941 cacagtccat ttacagacgg gaacgggctc tgaacttttc gcttgccagt gtggggattc
     6001 aggcgcaggg acgtacttcc gtcctgccgg taaattaccg taatacgaaa cgcattctgc
     6061 actttgctta tgcattctcc cgtgagtatt ttgagaagca tcagaaccgt gaaataccgt
     6121 ttgttcagcc acaggcgggc ggtgaagagg gcacagagcc ggaaattctg cgttgtgctt
     6181 ctgagagtga tgaggcagtg caggttgtgg gctggctgga gaaacgctat acactgtgcg
     6241 gtcactggag tgatatggct gttttgtgtc ctgcagagtt ttcagtgaaa catctgaaag
     6301 aggtcatgac acaacgcggg ataccttatg ccacctgctt tgacagtgaa ggtaagaaga
     6361 aatattcgcg acgcaaggat gttgtgcatt tactgaccta ccagagcagt aagggactgg
     6421 agtttccgta tgttgcggtt atcaatgctt ctttcgttca ctcaggtgct gcggatgagt
     6481 ctgaagttat tccggcgctt tatgttgcgt tcacccgggc tacacgggaa ttactggtga
     6541 cctgttatcg tgaaaacagt atcagtcgtc atcttgagga ttttgtataa gttgtgtttt
     6601 attattgctg atatgtaatc attcgttttt tgataatcta agatgggtat ttagcgagta
     6661 ttcagagttg aattaatatc ccccatgttt ttgatctgtc aatagctgtc cattacatgg
     6721 ggtgatcata tatcagtcgg taattactgg ataaagtcac cccctaaatg attactttat
     6781 tgtccatgcc ggactaaagt tttcctcctc aaatagttct gttaacccac ttatttgttt
     6841 taatctcagt acctcatctg tatccatgcc cagttcacta cctattcgga gatcatccca
     6901 tcccattcgg gaaagatctc ggacgatatc ggagagcgcg tctatctggt gctttcctct
     6961 ggccctgtta tgcctgatgg tcgaggcgat agagtaagct ttaaagttat tatcatgctt
     7021 aatgcatgtg acaggtaaat atccttttag ttttttctct agttttgttg tgcttttccc
     7081 aagcatttgt ctgtgaaatc cgtcaataac ggtgtatttc cccttttgag ctaaggtgac
     7141 aacaggttga gtgaatccat caaccaacag ggagtgtgtt aataaccttt tttcggaggg
     7201 agccatgacg ttaggattat agtcgtttgc ttcaattaga ttgtttttta tccatataac
     7261 acaatcaaca ggttctgcgt aaaaaggact actcctatgt aactcaatct ttattttatt
     7321 aattgcatca atgcgttcat cttctttctt aagagaatta aggtatacat ttatttcatt
     7381 tatgatttgt tcaatgctca tgtgtttcta tttcttttaa tttgtatatt tttcttgttt
     7441 taagatgagt tgttcttctt tctctacttt gtgttaatat ataagttgta aggttgtttt
     7501 gtgtttttat ttttggtgct ggaggagata atatgctcct catgataaac gatgtgattt
     7561 tcaaat
//
LOCUS       DAZFVO020000205         7525 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFVO020000205 DAZFVO020000000
VERSION     DAZFVO020000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.206"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHKNB_RS22595"
                     /old_locus_tag="pgaptmp_004519"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHKNB_RS22595"
                     /old_locus_tag="pgaptmp_004519"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HGU4197902.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVAMELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2136
                     /gene="gatZ"
                     /locus_tag="ACHKNB_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /pseudo
     CDS             879..2136
                     /gene="gatZ"
                     /locus_tag="ACHKNB_RS22600"
                     /old_locus_tag="pgaptmp_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            2146..2598
                     /gene="gatA"
                     /locus_tag="ACHKNB_RS22605"
                     /old_locus_tag="pgaptmp_004521"
     CDS             2146..2598
                     /gene="gatA"
                     /locus_tag="ACHKNB_RS22605"
                     /old_locus_tag="pgaptmp_004521"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HGU4197903.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2629..2913
                     /gene="gatB"
                     /locus_tag="ACHKNB_RS22610"
                     /old_locus_tag="pgaptmp_004522"
     CDS             2629..2913
                     /gene="gatB"
                     /locus_tag="ACHKNB_RS22610"
                     /old_locus_tag="pgaptmp_004522"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HGU4197904.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2917..4272
                     /locus_tag="ACHKNB_RS22615"
                     /old_locus_tag="pgaptmp_004523"
     CDS             2917..4272
                     /locus_tag="ACHKNB_RS22615"
                     /old_locus_tag="pgaptmp_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGU4197905.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIEVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4320..5360
                     /gene="gatD"
                     /locus_tag="ACHKNB_RS22620"
                     /old_locus_tag="pgaptmp_004524"
     CDS             4320..5360
                     /gene="gatD"
                     /locus_tag="ACHKNB_RS22620"
                     /old_locus_tag="pgaptmp_004524"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4197906.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            5466..6239
                     /locus_tag="ACHKNB_RS22625"
                     /old_locus_tag="pgaptmp_004525"
     CDS             5466..6239
                     /locus_tag="ACHKNB_RS22625"
                     /old_locus_tag="pgaptmp_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGU4197907.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6321..7220)
                     /gene="yegS"
                     /locus_tag="ACHKNB_RS22630"
                     /old_locus_tag="pgaptmp_004526"
     CDS             complement(6321..7220)
                     /gene="yegS"
                     /locus_tag="ACHKNB_RS22630"
                     /old_locus_tag="pgaptmp_004526"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HGU4197908.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1895 a         1689 c         1957 g         1984 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg gttatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aatggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaacaag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa agcatatgtt cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctgcgg aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc
     1381 accgaagttc cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat
     1441 gttgaagatg ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg
     1501 acagaggcgt taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac
     1561 agcaatatta tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac
     1621 acccgaatgg tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa
     1681 ttagtccgcg atcactttgc aatattgaaa gtcggtcccg cattaacctt tgctttacgc
     1741 gaggcgatat ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc
     1801 ggttgcctgg cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat
     1861 tatcgtacgg gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt
     1921 cgttattact ggccgcatag tcggattaaa aatagcgtcg aaacgatgat ggtgaatctt
     1981 gaaggcgtgg acaccccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc
     2041 attcagtccg gggaattatc agcaataccg catcagctga ttatggataa aatttatgat
     2101 gttttgcgcg cctatcgcta cggctgtgcg gaataaggac ggtatatgac taacctgttt
     2161 gttcgtagcg gaatttcttt tgtcgatcgt agcgaagttt taacccatat cggtaatgag
     2221 atgctcgcca aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca
     2281 gaattcccta ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg
     2341 attcatgcta agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag
     2401 caagcggatg atgataacga cgtggaggta tcgttggtta ttgcgttgat tgtggaaaat
     2461 ccgcagcagc aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc
     2521 gtcgagacac taatcactct tcctgaaacc cagttaaagg aatacttcac aaagtatgtt
     2581 ttagattcag acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag
     2641 attattgtcg cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa
     2701 gagttgtgtc agaatcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata
     2761 gaaacctata tggatggtgt gcatttgata tgcaccactg ccaaagtgga tcgtagtttt
     2821 ggcgatattc cgttagttca cggcatgcct tttatttctg gtgtcggtat cgaagcatta
     2881 caaaataaaa ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat
     2941 attctcgacc tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata
     3001 ttaggcatga aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt
     3061 ggcattggcc ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg
     3121 gcggaaaatt tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca
     3181 atgacctggg cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg
     3241 gcgatgttac tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat
     3301 atgaccttca ccggcgcgtt gctgcacctg gcaaccggtt catggatgat agggatggca
     3361 ggtgtggtaa ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc
     3421 cgaaatttct ttgagctgga aggtattgct attccgcacg gtacgtcggc gtatatgggg
     3481 ccgattgagg tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa
     3541 tttagcgccg acgatattca gcgcaaattt ggcccttttg gcgaacctgt caccgtgggt
     3601 tttgtgatgg ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag
     3661 ctggcggtaa aaacggcggc agtgatgctg ctgatgccac gggtgattaa acccatcatg
     3721 gatggtttaa cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt
     3781 caggagttcc tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg
     3841 gcaagcctga tttttatccc actcaccatt ttaattgctg tttgtgtgcc gggtaatcag
     3901 gtgctgccgt ttggcgatct tgccactatc ggcttctttg tggcgatggc ggtcgcggtg
     3961 catcgtggaa atctgttccg caccttaatc tcgggtgtca tcattatgag catcaccctg
     4021 tggatcgcga cgcaaactat tggtttgcac acccaactgg cggctaatgc tggggcgtta
     4081 aaagccgggg gtatggtggc ttcaatggat cagggcggtt ctcccattac ctggttactg
     4141 attcaggttt tctccccgca aaatattccc ggtttcatta ttatcggtgc aatttatctg
     4201 accggtattt tcatgacctg gcgtagagcg cgtggcttta ttaaacaaga gaaagtcgtt
     4261 ctcgcagaat aatttttacc tgagggggag gttatccccc ttaacaatca ggagttttta
     4321 tgaaatcagt ggtgaatgat actgatggta tcgtgcgcgt tgcagaaagc gtcattcctg
     4381 aaattaaaca tcaggatgag gtgcgggtaa aaattgccag ctcgggctta tgtggttccg
     4441 atttacccag gatatttaaa aatggtgcac attattatcc aataacgtta ggccatgaat
     4501 ttagcggcta tattgatgcg gtgggatccg gtgttgatga tttacaccct ggcgatgcgg
     4561 ttgcctgtgt gccgttatta ccctgtttta cttgtccaga gtgtctgaaa gggttttatt
     4621 cccagtgcgc aaaatatgat tttattggct cgcggcgtga tggtggattt gctgaatata
     4681 ttgtcgttaa gcgaaaaaat gtctttgctc tacccacgga tatgcctatt gaggatgggg
     4741 cttttattga gccgattacc gttggtctgc atgcttttca tttagcgcaa ggttgtgaga
     4801 ataaaaacgt tattattatt ggtgccggaa ccattggcct gctggctatt cagtgcgctg
     4861 tcgcgctggg agcaaagagt gtgacggcta tcgacattag ctcagaaaaa ctggcactgg
     4921 caaaatcttt cggtgcgatg caaacattta acagtagcga aatgagcgcg ccgcaaatgc
     4981 agagcgtttt acgcgaactg cgttttaatc agcttatcct cgagacggct ggcgtaccgc
     5041 aaactgtcga actggcggta gagattgccg gtcctcatgc ccaactggcg ctggtgggca
     5101 cgttgcatca ggatctgcat ttaacatcgg caacgtttgg caaaatattg cgtaaagagc
     5161 tgacggttat cggcagttgg atgaactatt ccagcccttg gccggggcag gagtgggaaa
     5221 cggcgagccg gttgctgaca gaacgtaagt taagcctgga gccattaatc gctcaccgtg
     5281 gaagctttga aagcttcacc caggcggtgc gtgacatcgc tcgtaatgcc atgccgggca
     5341 aagtgttgct cattccatga aaccgcgggc cagcgtgatg ctggcccggt attgtgcaaa
     5401 acagatcatt caccaatggt cccccttcgt ttacactagc cacaattgaa tatgggtaaa
     5461 tgacgatgaa ttcattcgag cgaaggaata agatcatcca attagtgaat gaacagggaa
     5521 ccgtgcttgt tcaggatctg gcgggagtat ttgctgcctc ggaagcgaca atccgtgccg
     5581 atttgcgctt tctcgaacaa aaaggcgtgg ttacgcgctt tcatggcggt gcggcgaaaa
     5641 taatgtccgg taatagtgaa accgagaccc aggaagtcgg gtttaaagag cgatttcagc
     5701 tcgccagcgc gcccaaaaac agaatagcgc aggcggcagt caaaatgatc catgaaggga
     5761 tgaccgttat tctcgacagc ggaagtacca caatgcttat cgccgaagga ttaatgaccg
     5821 ctaaaaatat cacggtgatt accaacagtc tcccggctgc gtttgccctt tccgaaaata
     5881 aagacattac tctggtcgtc tgcggtggca cggtccgcca taaaacgcga tctatgcatg
     5941 gatctattgc tgaacgttcc ctgcaggata ttaatgccga tttaatgttt gtcggtgctg
     6001 atggtattga tgcggttaat ggtattacga cctttaatga aggttattcg attagcggtg
     6061 cgatggtcac cgccgctaat aaagtgattg ccgttctcga ttcatcgaaa tttaaccgtc
     6121 gcggttttaa tcaggtactg ccaatcgaaa aaattgacat tattattacc gatgatgctg
     6181 tatcagaggt ggataaactg gcattgcaga agacacgggt aaaattaatt acggtgtagt
     6241 attgttctgt atgccggata aggtatttac accgcatccg gcatttcgga ttctccctgg
     6301 ttcaagagag aatagtttga ttaacgcaat aatggacaat ccggtggtaa tcgacaacgc
     6361 aacgccgccg gaagtatttc aatatggaag ttttgcccac tcaacggttc gccatcaaga
     6421 ttaaaagtga tttcgtgtgg ggcttgtata tcaaaccacg acgaagcgcc ttcgataata
     6481 ttcgggttat cttcgtcaga ttttaaggtt gatacgagag ccggaagaat ttcatcgccg
     6541 gtaaaaatgc gcagttgcag caagccatcg ttaattaacg cgttcgggca caattgttga
     6601 ccgccaccgg cctgacgccc gttaccaata ccaatgacca gggcgtcacc ttgccagtga
     6661 aagttttcac cgcggatttc acaacggtcc ggttgcagag tatccatgcg cattaagcca
     6721 tgaatgatgt aagagacgcc acccagcgcg gcttttaatt tttccggcgt ttctgtggta
     6781 atacgcgtcc caaatccgcc tgtcgccata ttaataaaac aggtttgttt gttgacctgc
     6841 gccatatcta tcgcaatggc gttaccggca attgccagtt tcagcgcctt atccagtgcc
     6901 tcaggaatcc ctacactggt ggcaaaatca ttggcggttc ctaatggcaa aattcccagc
     6961 gcgggtatgt catccccctc acactgaatc aacgccgtag aaacttcatt aatggtgcca
     7021 tcgccaccac cggcaatcac cgttgcgacg cccaacttcc gggcctcctc tacatatcgt
     7081 gcggcatcgc ctttctccca ggtgacccgc acatggatcg tcattccttc ctcacgcaac
     7141 agcataattg cttcgcgcaa gggtagattg tcagtacttt tgccattaag aatcagtaag
     7201 ctggcgggaa attctgccat gatcgtatgt gcctttatga ttggtctgta gatagtgtag
     7261 agcagaaaac aaaaggtgga gtcagaacag gatgaaagtc ggaggataaa aagtcagttc
     7321 aagtatggag gattcagtgg ttaccaccag gagcttgcaa tggggacagc gaatactaac
     7381 ggctgccacc gataaatttc aaaaaagagc atatacctaa tattcaacta aacagtggca
     7441 tcttcaatat aatatattaa agcccccatg gagttaccct gaagggcctc aatgtccgta
     7501 attcctactt atgtaggaaa tgttg
//
LOCUS       DAZFVO020000206         7475 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFVO020000206 DAZFVO020000000
VERSION     DAZFVO020000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.207"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            128..358
                     /gene="chaB"
                     /locus_tag="ACHKNB_RS22635"
                     /old_locus_tag="pgaptmp_004527"
     CDS             128..358
                     /gene="chaB"
                     /locus_tag="ACHKNB_RS22635"
                     /old_locus_tag="pgaptmp_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HGU4197909.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            516..1211
                     /gene="chaC"
                     /locus_tag="ACHKNB_RS22640"
                     /old_locus_tag="pgaptmp_004528"
     CDS             516..1211
                     /gene="chaC"
                     /locus_tag="ACHKNB_RS22640"
                     /old_locus_tag="pgaptmp_004528"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HGU4197910.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1255..1608)
                     /gene="ychN"
                     /locus_tag="ACHKNB_RS22645"
                     /old_locus_tag="pgaptmp_004529"
     CDS             complement(1255..1608)
                     /gene="ychN"
                     /locus_tag="ACHKNB_RS22645"
                     /old_locus_tag="pgaptmp_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HGU4197911.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1793..3187
                     /gene="ychO"
                     /locus_tag="ACHKNB_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /gene_synonym="iatA"
     CDS             1793..3187
                     /gene="ychO"
                     /locus_tag="ACHKNB_RS22650"
                     /old_locus_tag="pgaptmp_004530"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HGU4197912.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3188..3838)
                     /gene="narL"
                     /locus_tag="ACHKNB_RS22655"
                     /old_locus_tag="pgaptmp_004531"
     CDS             complement(3188..3838)
                     /gene="narL"
                     /locus_tag="ACHKNB_RS22655"
                     /old_locus_tag="pgaptmp_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HGU4197913.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3831..5627)
                     /gene="narX"
                     /locus_tag="ACHKNB_RS22660"
                     /old_locus_tag="pgaptmp_004532"
     CDS             complement(3831..5627)
                     /gene="narX"
                     /locus_tag="ACHKNB_RS22660"
                     /old_locus_tag="pgaptmp_004532"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HGU4197914.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5966..7357
                     /gene="narK"
                     /locus_tag="ACHKNB_RS22665"
                     /old_locus_tag="pgaptmp_004533"
     CDS             5966..7357
                     /gene="narK"
                     /locus_tag="ACHKNB_RS22665"
                     /old_locus_tag="pgaptmp_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HGU4197915.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWTVYGRHSKK"
BASE COUNT         1741 a         1819 c         1968 g         1947 t
ORIGIN      
        1 tattacgttt agacaatgtt tgttttagcg tttcttaaga acagtctgac atgaaaattc
       61 ttatgttttg gcaagagtag atattgttga ccacacttaa tgttcaactt tgtaaaagga
      121 gtcaacgatg ccgtataaaa cgaaaagcga tctgccggaa agcgtaaagc acgttctacc
      181 gtctcatgcc caggatatct ataaagaagc gttcaacagc gcatgggatc aatataaaga
      241 taaagacgat cggcgtgatg acgccagtcg cgaagaaacc gcgcataaag tggcctgggc
      301 tgctgtgaag catgaatatg ccaaagggga tgatgataaa tggcataaaa aatcgtaaaa
      361 ccggtagctt agttaaagct attcgtgcgg tgttgccttg caagtggtcc gtggattgca
      421 tattgtcccg ttagtggttt caaaatgagc agtaaaaatg tccggaagac accaaaaagt
      481 tgtcgcaggg aagtatgcag tggcggaggt gtaaggtgat aacgcgtgat ttcttgatga
      541 atgccgattg taaaacggca tttggtgcca ttgaagaatc actcttatgg tcagcagagc
      601 aacgggcggc ttcgctggcg gcgacgctgg cttgtcgacc tgatgaggga ccggtgtgga
      661 tcttcggtta tggatcgttg atgtggaatc cggcactgga gtttaccgag tcgtgcaccg
      721 gtacactggt tggatggcat cgcgcgtttt gcctgcgcct gaccgccggg cgcgggactg
      781 cgcaccagcc gggacggatg cttgcactga aagagggcgg acgcactaca ggcgtcgcct
      841 atcgactgcc agaagagacg ctggagcagg aactaaccct gttgtggaag cgcgagatga
      901 ttaccggctg ttatctgcca acctggtgtc agcttgatct tgatgatgga tgcacagtaa
      961 acgccattgt gtttattatg gacccgcgac atccagaata tgaatctgac actcgcgctc
     1021 aggtcatcgc gccgttgatt gcggcggcga gcggtccgct gggaaccaac gcacaatacc
     1081 tgttttcact ggaacaggag cttatcaaac tgggaatgca ggacgatggg ctgaatgatt
     1141 tgctggtatc ggtaaaaaaa ctgctggcgg agaattatcc ggatggtgtg ttacggccgg
     1201 gattcgcctg agtaaacttc cctcatagtg gggcgtcaga cgccccctca aacattaaaa
     1261 tgtgagcact ttatcggctg acagcgtcca ttgcgccagt tccacaagag taccgatttc
     1321 caccccatca ataagaggaa gtgtgctaat cccgcgcccg tcggtacagg ttttgcacaa
     1381 tttcaccggt acattctgag cggtaaggat ctccagcatt tgctgaatgt tgtagccttc
     1441 ccctggtttt tgcccgcgca acccggctgt gaccgcatca gacatcagga acagacgcag
     1501 atccagattg ctctcctgct ctcgtaacgc aatggccagc cgcaagctgt taaacaagga
     1561 ttcgctcccg taaggtgcgc cattggcaac gatcacgatt ttttgcatta tttactcctg
     1621 tattcaggga attagacatt catcttctat cttactgctt ctgcagcgtc tgaccaatcg
     1681 gtcacatttt taaggatttt cctgaaagcg cgagaaaata cgacaaaagt tgccagtaat
     1741 cgttattctt taaggctatg gtttttcatt attaccggaa gttaccgacg ttttgagccg
     1801 tttcgttcct cgcattatcc cgttttattt actcttgctt gtagcaggcg gtacagctaa
     1861 cgcacaatct accttcgagc aaaaagcggc aaatcccttt gataataaca atgatggtct
     1921 gccggattta ggcatggctc ccgaaaatca tgatggggaa aaacactttg ctgaaattgt
     1981 gaaagatttc ggcgaaacca gtatgaatga taacgggctg gatactggcg agcaggcaaa
     2041 agctttcgca ttgggaaaag tccgcgacgc gcttagtcaa caggttaatc agcacgtaga
     2101 gtcctggcta tcaccgtggg gaaatgccag tgttgacgtc aaagtggata acgaaggaca
     2161 tttcaccggc agtcgtggaa gctggtttgt gccgttacaa gataatgatc gttatctcac
     2221 ctggagccag cttggtctta ctcagcagga tgatgggttg gtgagcaatg tgggcgttgg
     2281 gcaacgctgg gcgcgcggca actggctggt gggttataac actttttatg acaacttgct
     2341 ggacgaaaat cttcagcgag cgggctttgg tgccgaagcg tggggcgaat atttgcgact
     2401 atcggcaaac ttttatcagc cgtttgctgc atggcatgaa cagacagcca cgcaggaaca
     2461 acggatggcg cgcgggtatg acctgacagc ccggatgcgc atgccgttct atcaacacct
     2521 caataccagt gtcagcgtag aacagtattt tggtgatcgt gttgatttgt ttaactctgg
     2581 tacgggttat cacaatcccg tcgcgttgag tctgggatta aattacaccc ctgtgccatt
     2641 agtcactgtg acggcccagc ataaacaggg tgaaagtggc gagaatcaaa ataacctcgg
     2701 gctgaatctt aattaccgct ttggtgtacc gctcaaaaaa caactttctg cgggtgaggt
     2761 tgccgaaagt cagtcgttac gtggtagtcg ctatgacaat ccgcagcgaa ataatctacc
     2821 gactcttgag taccgacagc gaaaaacgtt aacggtgttt ctggcgacac cgccgtggga
     2881 tctaaaacct ggcgaaacag tgccgctgaa attacaaatc cgcagtcgtt acggtattcg
     2941 gcaactgatt tggcagggcg atacgcagat attaagtttg acgccgggcg cacaagccaa
     3001 cagcgcggag ggctggacgc tgatcatgcc tgactggcag aacggggaag gggcaagcaa
     3061 tcactggcga ttgtcggtgg tggtggaaga taaccagggg cagcgtgtct cctccaatga
     3121 gatcacgcta acgcttgtcg aaccgttcga tgcattgtca aacgacgaac tgcgctggga
     3181 accgtaatca gaaaatgcgc tcctgatgca cccataccgc tgcttccacg cgagacttga
     3241 gcttcatttt cttcagcatg tgcttgacgt gcacttttac tgtgctttcg gtgatatcca
     3301 ggcggcgggc aatcatcttg ttcggcaaac cctgggcaat cagcttgaga atatcgcgct
     3361 cgcgtggggt taactggtta acatcgcgct cagtagtggc acggttagcg cgcaagctgg
     3421 cggccagaac aggcgttaat gcttcgctta ataccatttc gccagcagct gcctgatgca
     3481 atgctttcag cagatcttcc ggttccatat cttttaacag atagccatcc gcgccgcgtt
     3541 tcagtgcggt gaccacatct tcttcatggt tagagacgct gaataccaca atgcgccctg
     3601 agagggactt ttcgcgcagt ttatccagcg tttccagacc gttcatgccg ggcatattga
     3661 gatctaacag gatcagatcg ggatcaagag attccgccag ttcaataccc tgttcgccat
     3721 tactcgcttc gccaaccacg gtgatatctg gtgccatact gataagctgt tttacgccag
     3781 ttcgcagcat cgggtgatcg tcaatcagca ggatagtagc cggttcctga ttactcatgg
     3841 gtatctcctt ggacgtctgt gaaagttttt tcgggaataa aggtgaccac cacttcggtg
     3901 ccacctgatt cacgacggcg gacgcggcaa tcgcctcgta aactttgcgc acggtcgcgc
     3961 attattatca tgccgtagtg attgctgcgg atggcatttt caggcacgcc gcagccgtta
     4021 tcctggacgg tcagtttgac ctgattatcg ttttgcgcca ccgtcaccac gacttcactc
     4081 gcttgcgaat gtttgagggc gttacttaat gcctcacggg caatttgcaa caagtggatt
     4141 gcctgatgcg aaggcaccag gcgaggcggc aattgataat ccagcttcac cgggaagcca
     4201 aatttggcgc tgtactcttc gcaactcgcc tccagcgccg gacgtaatcc aggctcggtg
     4261 agctgcaagc ggaatgtggt gagcaattca cgcaactgcg cccaggatgc attcagttcg
     4321 ttacggatct gacttaacag ttcgcggctg ctttctggca gcgcatcgcc ctgcatctgt
     4381 aaacaactca cctgcatctt catgcaagag agagattggg caatagaatc atgcagttcg
     4441 cgcgcaatgg tggcacgctc ttccatcacg atcaactgtt gctgacgttc ctgatggcga
     4501 tccagcgcca gcgtggcggt gagttgttca accagggtat ccaccagttg ttgttgatca
     4561 tggctaagat gacgcccctg cggcagggtc gccagcaaaa taccgtactg cgtatgagag
     4621 tcagccagcc gccacttcag ggttgtgccg cgatcaccaa cgggtaatac gccgcgcggg
     4681 cagagctggc agcctttatc atcacaagtc atatctggtt ggcaggtaaa ctcctgatga
     4741 ttctcttcat catcagtatc ataaacccgc agttcgatat cacgtagcag ggttaaattc
     4801 tgtaagccgt tgagtacagg tgacaggcgt tcacacagcg gggcttggga atgcaaacgg
     4861 cggttagcct gccataaaaa agagaggatc tgatttttat gctccagccc ggcggttttc
     4921 tcctgaaccc gctgctcaag tacggcataa ctttcggcca gttctgcaga catattgttc
     4981 aacgcagttc caagcatcgc catttcgttg cgcccgctga tgtttgcgcg ttgggtaaaa
     5041 tcgcgatgac tgacggcact cgccattgcc agcagttgcc gccacggttg tagcagtcgc
     5101 gcccgcaacc agataatagt gaacaccagt aaaagtgcca taaataccgc cattacccga
     5161 tggaccagta ccactgtctc gatgcgcatt tccgtggtgc ggtcaaaacc agataccagt
     5221 tgatcaagcc cggcaacaaa ctggctgaca tccgctgaca ccgtttctcg gttttgtgca
     5281 cgcatcaacg cagggatcag ttcattacgc cagtaatctt gtaaaccctg taattgcgcc
     5341 agttgtccgt ctcgttctgc tgctcgagtc aactcggcgc taaatgccgt ttgttccatc
     5401 tctttaatta agggcttgtc tttctcgctt aatggcactg ctgccaacag acggtaactt
     5461 tgcatgcgca gcgatcccgc tttgttgatc gcatgggcgc tgccctgaac gccttgcacc
     5521 agccagccag aaaccgccat ccctgccagt ccaatagcag tagaaagcaa cacaataagc
     5581 gcaacctgat taaccagggt gagcggagag agacaacgtt taagcatgta aacctcttcc
     5641 ttcaggcttt aaatgagcaa taaccttaat gaatgtgacg atacattctg gaatggcagt
     5701 attctcggct attggctgaa gtatacccat acccggaaag agttactcct tatttgccgt
     5761 gtggttagtc gctttacatc ggtaagggta gggattttac agcactgtga aaaatctcat
     5821 aatttttatg aaatcactgt actcactatg ggtaatgata aatatcaatg atagataaag
     5881 ttatcttatc gtttgattta catcaaattg cctttagcta cagacactaa ggtggcagac
     5941 atcgaaacga gtatcagagg tgtctatgag tcactcatcc gcccccgaaa gggctactgg
     6001 agctgtcatt acagattggc gaccggaaga tcctgcgttc tggcaacaac gcggtcaacg
     6061 tattgccagc cgcaacctgt ggatttccgt tccctgtctg ctgctggcgt tttgcgtatg
     6121 gatgttgttc agcgctgttg cggtgaactt accgaaagtc ggctttaatt ttacgacaga
     6181 tcagctattt atgctgactg cgctaccttc agtttctggc gcgttattac gtgttccata
     6241 ctcctttatg gttccaatct tcggtggtcg tcgctggacg gcgttcagca ccggtattct
     6301 gattattcct tgtgtctggc tgggttttgc cgtgcaggat acctccacgc cttatagcgt
     6361 cttcatcatc atctctctgc tgtgcggctt tgctggcgcg aacttcgctt ccagtatggc
     6421 aaacatcagc ttcttctttc cgaaacagaa gcagggtggc gcgctgggcc tgaatggtgg
     6481 tctgggcaac atgggcgtta gcgttatgca gttggttgct ccgctggtgg tatcactgtc
     6541 gattttcgca gtatttggta gccagggcgt caaacagccg gatgggactg agctgtatct
     6601 ggcgaatgcg tcctggatat gggtgccgtt ccttgccatc ttcaccattg cggcgtggtt
     6661 tggcatgaac gatcttgcta cctcgaaagc ctccatcaag gagcagttgc cggtactcaa
     6721 acggggtcat ctgtggatta tgagcctgct gtatctggca accttcggct cctttatcgg
     6781 cttctccgcg ggctttgcga tgctgtcaaa aacgcagttc ccggatgttc agattctgca
     6841 atacgctttc ttcgggccgt ttattggtgc gctggcgcgt tctgcaggtg gtgcattatc
     6901 tgaccgtctg ggcggaactc gtgtcacgct ggtgaacttt attctgatgg cgattttcag
     6961 cggcctgctg ttcctgacct taccgactga cgggcagggc ggaagcttca tggcgttctt
     7021 cgcggtcttc ctggcgctgt tcctgacagc tgggctgggt agtggttcca ctttccagat
     7081 gatttcagtg atcttccgta aactgacaat ggatcgcgtg aaagcagaag ggggttctga
     7141 cgaacgtgcg atgcgtgaag cggcaaccga cacggcggcg gcgttgggtt tcatctctgc
     7201 gattggcgcg attggtggct tctttatccc gaaagcgttt ggtagctcgc tggcattaac
     7261 gggttcgcca gtcggcgcaa tgaaggtatt tttgattttc tatatcgcct gcgtagtgat
     7321 tacctggacg gtatatggtc ggcattctaa aaaataaatc ttaatatttg attatccttt
     7381 gcgcggcatg atgtcgcgct ttttttatgc gttatttagt tacaacatac taatgttata
     7441 tggcttattt cgccggattt cattaagagc catta
//
LOCUS       DAZFVO020000207         7431 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFVO020000207 DAZFVO020000000
VERSION     DAZFVO020000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.208"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..104)
                     /locus_tag="ACHKNB_RS22670"
                     /old_locus_tag="pgaptmp_004534"
     CDS             complement(<1..104)
                     /locus_tag="ACHKNB_RS22670"
                     /old_locus_tag="pgaptmp_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197916.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIV"
     gene            complement(88..4326)
                     /locus_tag="ACHKNB_RS22675"
                     /old_locus_tag="pgaptmp_004535"
     CDS             complement(88..4326)
                     /locus_tag="ACHKNB_RS22675"
                     /old_locus_tag="pgaptmp_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU4197917.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQYSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRASSLSSPASPRSV
                     SSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     AYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTRKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPA
                     GQLQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIR
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGV
                     IRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTG
                     WMSLSRKPEETWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCR
                     SLAEKIQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLA
                     AQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQP
                     YRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMG
                     LDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCY
                     LMCRMTKTFGSTIADVIGKNHEAAGNRQGQPAKERIMDLKNNTVGIACGDFSAKCSDA
                     CIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
     gene            complement(4402..6543)
                     /locus_tag="ACHKNB_RS22680"
                     /old_locus_tag="pgaptmp_004536"
     CDS             complement(4402..6543)
                     /locus_tag="ACHKNB_RS22680"
                     /old_locus_tag="pgaptmp_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4197918.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDHSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARPVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(6753..7271)
                     /locus_tag="ACHKNB_RS22685"
                     /old_locus_tag="pgaptmp_004537"
     CDS             complement(6753..7271)
                     /locus_tag="ACHKNB_RS22685"
                     /old_locus_tag="pgaptmp_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HGU4197919.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         1401 a         2340 c         1929 g         1761 t
ORIGIN      
        1 aatacgatgt cagaatttga attgttatca aacccaaggt aaacaacaaa taaacaaagg
       61 gtaattaaaa aaatgacaag tttgcctcta ataatttgaa gcatctgaac tcccttgctt
      121 tgcttttatt ggattatctg cttttatacc atctaacccg aagagttgcc cagtgttata
      181 tttttcaata catgcatcgc tacatttggc agaaaaatcg ccacaagcaa taccgacagt
      241 gttattttta agatccatga ttctttcttt agctggctga ccttgtctat tccctgccgc
      301 ctcatgattt ttacctatca cgtcagctat tgttgatcca aatgtcttag tcattcgaca
      361 catcaagtaa caatgtctca ttgcatcggc ggccccatta tgagctccgc caacaatagc
      421 atccatatca taaccgaact ggtaagcact gttagtaatg ctgtttgcag caatcaatcc
      481 aggtacacca gatacggcat aaattagtcc acctgatgtc atattctcaa tcgcatctaa
      541 ccccattggg tctatcactt gtatgggatt caacggatac tgatacatat tccatccacc
      601 tctcaaccca atcgggtccg gggtgatata ccgcccctgc aacggatcgt agtaccggtg
      661 acggttatag tacagccccg actccttatc atactgctgc cccggcagcc ggtacggctg
      721 gtgcaggtga tgcgggttct cctcattaag ctggttgccc cattcatcat attccgcgct
      781 ccacgccgta ttgccgtctt cactgataag cgccagcggc aggccccggt ggtcacagtg
      841 ataaagatgt atttttcgct caggcaaata aaccggctct atctgtgccg ccagccgctc
      901 caccgtcagc ccgcactgcg caagccactg gcggctttca ctgctcacgc agtttgcccg
      961 gatttccccc tccagcctgt ccagcagccc caccagttca gccgggaaca ccacaccgtg
     1021 accgtcttcg ctcccttcct gctgtatctt ctccgccagg ctgcggcact gcgctttctc
     1081 ccgttcaccg ttgtctgttt cgatgcggat gagcggcgcg aagctccccg gctggtatac
     1141 cgtctggata cgcgtggtgt cggtctgtac cgtcgtcagc ctgtcgccgt cccagccgta
     1201 ccatgtctcc tccggtttac gcgacagcga catccacccc gtcaggtcac gctcccgccg
     1261 ccatacccgt ttcgccattc gccgtcccag cgggtcgtag aggtagcggc tctcgaccag
     1321 tggctcgcca tgctgtatcc gcgtgtggaa caccaggcgg tgctggctgt cgtagtggta
     1381 gtggtgggtc cgctcgtcgt ccgtccgtat cacacccgtc gggatgcggt ccgtcttctc
     1441 cgtcagtctg ccgtattcat catagtggta aacatagtgc gcatcctctg cgatgcggtt
     1501 atccggccac gccgtgagcg tgctgtccgg gtgtagctcc gggtccggca ggcggtttcc
     1561 cgccgggtcg gtggcatacg ggatgcggat gtccaggtcc ggtgcgaggg tgcgcacgct
     1621 ctccagcctg cccgtggcac tgtagccgta ttcccgcgtc tgtcgcggac cgctgatgcg
     1681 caccaggtcg ccgttgtcat tccacccgta gtcacggtca tacaccaggc tgttcaggtg
     1741 ctggctctgt aactggcctg cgggggtgta tgtgctggtc agttcatatg cggcattact
     1801 gcctgccctg ctgccgaagc tgcgcaccgt ctcacggtgc agcctgtccc gcgtatactc
     1861 gaccagcggc gtcccgccca gcttcatgcc cgccaggtaa ccgctgccgt aggtcagcca
     1921 ctccaccggc ggcaggctgt ccggcgtgac gcggtttgcc agcccctgtt cgttgtaggc
     1981 gtgtttcgtc tcatgctgcc acagcagttc ccccgtctcc gggttctcca ccgtctgccg
     2041 ttcgcccgtc aggcggccct tatcgtcgta gccgtagtgg accgacacgc ggtggccttc
     2101 actggtatgg ctgagggtgg ttaaccaccc gtgctcatca tactgccact gctctgccgg
     2161 gtcgccgttc accgtccggt gcgtgatgcg gtccgacgca tcgtagtgcc agagggtgat
     2221 aagcccctcg tcttcactct gtgtgagttt tctggtcagg tcatagtggt aacgttgcgt
     2281 ccggccgtca aaaccgcgct gttcagtcag cctgtcaacc gggtcatacc ggaacgtgga
     2341 ctggctgccg ttctcgttgg tcagcacggt gatgcgcccg gcagcgtcat accccatgct
     2401 gcgcgtcaga ccgccttccg tcacactcac cggacgcccg cgtttatcat atgcgatggt
     2461 gctgcgttta ccgtccggtg ataccgttgc tgtcaggtct cctgcggcgc tgtactcata
     2521 acgggtttca cgcccctgcg catccttctg actgaccatt tgcccacgcg ggttataact
     2581 gctgtaagtg ctgatgcctt cttcccggtg aacggcgatt tgttgaccgt accggtcgta
     2641 ctcataccgc gtcgtgtacc ccgagcagtc cgtaaaggcc agcagttgac cgtaacggct
     2701 ccatgccatc tgttttgtac tgcccgtcgc gtcctgtatc cctgtcggca gctcactcgc
     2761 cgggtcatca tagctgtaac tcgtcgtctc accgctgcgt gaggtctccg ccgccagcct
     2821 tcccttctca tcatactccc ggctgctgcg aagcccgtcc gggtacgtca ccgacgtcac
     2881 ctgccgctgg ctgttatagc cataccgtac cgtcctgcca tccggcagta tcactgaagt
     2941 cagctttcca gacgccatat gcagccggta ctccgtccgc cgccccgccg catccgtctg
     3001 cgccttcagc ctccccgcct catcatactc gctgcgggtg atgctgccgt ccgcatgctc
     3061 cttcttcacc acacgcttca ggccgccttc gccctccgtg tacagcacct cccgccggtt
     3121 caggctgtcc gtgatggtca cacggtcctg cccgtactca aagcgatagt ccagcccctc
     3181 cgggttgacc tgctccgtca cccgcccggt atcatcatac cggtagcggc tctccggtct
     3241 ccctgcataa tggtgcgcca ccatccgccc ggcgtgctcc gcatcataag taaacccgcg
     3301 cacctgcgtt ccgctgcggt catacaccgc ccgcagttct ccgctggccg tgtacgtgta
     3361 gcgcgccagc ggcgcagtcg gctgttcatc cgggtatgcc gggtcgtgtg tcagccatac
     3421 cgcctccagc cggataccgt tatcggcacc gtactctgta ccggcgggca gcgtgtcggg
     3481 gaaaacctga gaagaggaaa cagaacgggg actggccggg gaagataagg aagaggcgcg
     3541 ctgtttacgg aaggcttccg cccgctgcgc ctgcgtggtc agcgccaggt gaaaacggcg
     3601 gcccgcgccg tccgtcaccc ccgtcaccgc gcccgccacg tcacccttag ctgcccggtg
     3661 aaaggccagc gtccgcccga agccgtccac cacgcccgtc agcacccggt atgcgggcgg
     3721 ctccggcggc agcacctcgt ccgcccccgg cacccgctcc ggccagctga gtatccacca
     3781 cggcccctga aggctgtttg tcgccaggta cacatgcgga ctcaggcgaa catcttccgg
     3841 cagcacctgc cagagcgcgc ttagcggctg cgaactgtac tgcgccgcta ctccgccccg
     3901 cgccagccag agtgactcgc tgcggctgta gcttatctcg ccgggaaaca gcggctcaaa
     3961 gtgaatgctc ctgccgccgc tgtcgttgag tatcaggcct tcatcgcgta tctgtaagcg
     4021 gatatcgaag ggcgctttcc agccgggacc aaacaccccc accggcgcgg gtgttctggt
     4081 ccggtagctg ctgtaggcgc gggaaagaat aaacggcagc gggccgggca gcgcaaggtc
     4141 cgtctcgccc ggcagcacct tcgcacccag cagcgggttc accgggttag cataggtaat
     4201 ccctcccgga cataccgaac aggccactcc cgtcggcgca cctatcagca cccccgctga
     4261 accctgcaca atatccagtc ccttgcgggt catatcaccc tgccgcgccg ccggttttcc
     4321 gcccatgaat caattccttc tgcttttaat atggtttatc tggtcactgc accagttccg
     4381 ttttaatttt ttgcacctga ctcaaaattg ccgcatatcc ggcattccgg ggggtaaatc
     4441 tttcggcaca ggcgcatccg gtacaggttg tgtggcacca cagttccagt taatcactgg
     4501 cgcatcaccg ttcacatccg actccccttc cagatgaatg tgcgtaccat tgagaaatat
     4561 gcttccgtcc ttcgtcagca ccggccttgc cttaccacag cagagttcaa gatgctcacc
     4621 ggcagaataa actgctgttt gtccggtgtt ttccttcatt gtcttgccca ccgagaaggt
     4681 gacgttattc cccacattga cgctgtagcc cattcccacc atcagtgatt tatgcagccc
     4741 tacctgtgag gactgcaaca acgccaccga ggtattcata atgccgccta ccgtcgtctg
     4801 gtaagccaca cccaccgtca gcgcacgaat gatccccacc ttttcaacct ggtttgatcc
     4861 cactttgata atctggttac tgcccaccgt ttccacctgg ttgacaccaa ccgtctgtat
     4921 ctgattaacg ccaatgttct ggatctggtt attggtaacg gtgatcgcct ggttgttacc
     4981 tattttttcg gcatggttgt ttatcacatc agtggtgcgg ttattcagca cctcggtgtt
     5041 catgttcttc tgcgcgtgga tgtagacctg ttctttccct gtcgcatcgt caaacttcag
     5101 ttcattaaac ccgctgccct tataggtttt cgagcggatc gtcatctgcg tcttcgtccc
     5161 cggcaggctg ccgggtgtgc ggttttcatg gtggtaggta cgccccataa taatcggctg
     5221 gtccgggtcg ccgttgagga agtccacaat gacctcctga cccacacgcg gtatcgccag
     5281 gttaccaaag ccggtgcctg cccacgcctg tgccacacgg atccagcatg aactttcctg
     5341 gtttgacggg ttatagcggt cccagttaaa tttcacccgc acgcggccat gttcatcaca
     5401 gaagatttcc tcgcctgccg gtcccgtcac gacggcgctc tgcgggccgt ccaccagcgg
     5461 tttcagcaac ggctgtggcc gccatgtccg gtctgccggg attaccgcaa agtggttatc
     5521 cagcgtggtg cctgaacccc tgcgtcccgg taccgcctgc ggctgttcgc cgtgaagatc
     5581 gctcgccacc acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat
     5641 tcgccgtccc ggccatatct ccggcgaacg gcttgttccg cgcgccactt ctgcgttgtt
     5701 gcgccagcca tccatctgcc agcgggcaaa gttctgcccg tgggcaccct tgaaacgtcc
     5761 ggggtagtca tacacttcat actgtgtgcg ctggtaatcc tggtactggc cttcctgatc
     5821 aaaacgccct gcccagccgg gtcgtttaaa ggtgtagtct ttggtcacca cggaagaagg
     5881 gcggatttgt gcgctgtagc gaaactggct gatgcagagg gtgctcacct cggtacgggt
     5941 gttcggattc caggggatct caaaggactc cggcagataa cgcacggtat cgcacaggac
     6001 caggctgtgg tcggtacttt tttgcgcgtg ctcctcataa aagaagatgc cttcctccgc
     6061 cgccatccgg cacaggaaat cgtaatcagt ctcaccatac tggacacaaa actcacggga
     6121 aggatgtggc tcgctgaaca gcgggctcca ctcggtcacc ccgttttcct gcaatatcgt
     6181 gccgaggatg ctttcgatgt cctcattctg gaagatacgg aagttctggc gcagccctgt
     6241 gcgccacagc ggtgggcaca ccttcatgct gtacagcatc tggtttttgt cgttctcccc
     6301 cagttcaaac caggtcacca tacctttcac ccggcgctgt acgtcatcgc cctgccatat
     6361 cgtcaggtag gccattttgt cgaggatctg ctggaattca agggagagaa actgctggct
     6421 gaccagagag agatcgaggg aaaaaagcga agagagtgac tggttcagat gaaaggagac
     6481 caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta atccggttga
     6541 catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg gctgtgacag
     6601 tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcag ccgccatctc
     6661 ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca aaaacagcat
     6721 ccggcctgat agccggatgc tgttggctta acttatgctt ccagcggtgc gcgccagtcg
     6781 tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta ggccagtgaa
     6841 acttcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg catatgcagt
     6901 ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc ctgtttgcct
     6961 tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc ggaggccagt
     7021 gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa aggcttgtga
     7081 gcacgctgac ccgcaggctg accggactga ggatcggtcg ggacagtaac gttatgcagg
     7141 aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc gacagaatca
     7201 gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga gatataacac
     7261 ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca atgacctgcc
     7321 gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt ttcagtattt
     7381 tttacataca aaccgagcta gaatttacgg ttgtcgtaaa aactattttt a
//
LOCUS       DAZFVO020000208         7409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFVO020000208 DAZFVO020000000
VERSION     DAZFVO020000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.209"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            77..1132
                     /gene="phoE"
                     /locus_tag="ACHKNB_RS22690"
                     /old_locus_tag="pgaptmp_004538"
     CDS             77..1132
                     /gene="phoE"
                     /locus_tag="ACHKNB_RS22690"
                     /old_locus_tag="pgaptmp_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4197920.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKNB_RS22695"
                     /old_locus_tag="pgaptmp_004539"
     CDS             complement(1171..1572)
                     /gene="crl"
                     /locus_tag="ACHKNB_RS22695"
                     /old_locus_tag="pgaptmp_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4197921.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKNB_RS22700"
                     /old_locus_tag="pgaptmp_004540"
     CDS             complement(1630..2874)
                     /gene="frsA"
                     /locus_tag="ACHKNB_RS22700"
                     /old_locus_tag="pgaptmp_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4197922.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKNB_RS22705"
                     /old_locus_tag="pgaptmp_004541"
     CDS             complement(2966..3424)
                     /gene="gpt"
                     /locus_tag="ACHKNB_RS22705"
                     /old_locus_tag="pgaptmp_004541"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4197923.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKNB_RS22710"
                     /old_locus_tag="pgaptmp_004542"
     CDS             3685..5142
                     /gene="pepD"
                     /locus_tag="ACHKNB_RS22710"
                     /old_locus_tag="pgaptmp_004542"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4197924.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5199..5813)
                     /gene="prfH"
                     /locus_tag="ACHKNB_RS22715"
                     /old_locus_tag="pgaptmp_004543"
     CDS             complement(5199..5813)
                     /gene="prfH"
                     /locus_tag="ACHKNB_RS22715"
                     /old_locus_tag="pgaptmp_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4197925.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5810..6949)
                     /locus_tag="ACHKNB_RS22720"
                     /old_locus_tag="pgaptmp_004544"
     CDS             complement(5810..6949)
                     /locus_tag="ACHKNB_RS22720"
                     /old_locus_tag="pgaptmp_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4197926.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7195..>7409)
                     /locus_tag="ACHKNB_RS22725"
                     /old_locus_tag="pgaptmp_004545"
     CDS             complement(7195..>7409)
                     /locus_tag="ACHKNB_RS22725"
                     /old_locus_tag="pgaptmp_004545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGU4197927.1"
                     /translation="LFVDPEYTRRGVASALLKPLIKSESELTVDASITAKPFFERYGF
                     QTVKQQRVECRGEWFINFYMRYKQQH"
BASE COUNT         1913 a         1888 c         1799 g         1809 t
ORIGIN      
        1 caggttaaaa actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt
       61 aaaatcagga atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc
      121 atctgcatcc gtacaggccg cagaaatata taacaaagac ggtaataaac tggatgtcta
      181 tggcaaagtt aaagccatgc attatatgag tgataacgac agtaaagatg gcgaccagag
      241 ttatatccgt tttggtttta aaggcgaaac acaaattaac gatcaactga ctggctatgg
      301 ccgttgggaa gcggagtttg ccggaaataa agcggagagt gatactgcac agcaaaaaac
      361 gcgtctcgct tttgccggat tgaagtataa agatttgggt tctttcgact atggccgtaa
      421 cctgggcgcg ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga
      481 ctcctcggcg cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg
      541 gaacaccgac ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa
      601 aaacgaaaac cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata
      661 tgactttggt ggcagcgatt tcgccattag tggtgcctat accaactcag atcgcaccaa
      721 cgagcagaac ctgcaaagcc gtggcactgg caagcgtgca gaagcatggg caacaggtct
      781 gaaatacgat gccaataata tttatctggc aactttttat tctgaaacac gcaaaatgac
      841 gccaataact ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca
      901 gtttgacttt ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga
      961 aggtatcggt gatgaagatc tggtcaatta tatcgatgtc ggggctacat attatttcaa
     1021 caaaaatatg tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt
     1081 gaatattaat aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatgattatt
     1141 gccggatgtg atgcatccgg cacatttcac tcacgccgtt aacttcaccg gctcgtcacg
     1201 aaaatcatcc gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc
     1261 gtgaaactca cgcagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac
     1321 gctcttccag tcgcctgctt tatcgaacag accaaactgg tagctgtagg taaaacggga
     1381 ttcctgcgct tccagctcca tccaccagcc ccagaattca cgcacttccg gtgccggttt
     1441 cacgttgacg catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc
     1501 acgaatatac gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact
     1561 cggtaacgtc attgctatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaaattt
     1621 agcaaatttt taacacaagc gtttttcgat ccagccggtg atttcctgaa gacctttgtc
     1681 aaaattccga tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga
     1741 ggtgattaag cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa
     1801 cattggcgtt gggcagcgac gtcccagcaa tccttgcact tttaatgaat agcgattcag
     1861 ctccacgcgc aacgcgtcat cggaagcatc atgcatcccc aaacgactcg ccagaacgtc
     1921 aagatacatt tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac
     1981 cggaccaaga caggcaaccg ctttcagacg cggcgattca aggtatgcca gacgcacggc
     2041 aacgttagcg ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac
     2101 gttaggcagc gcctttaaga cgtgctgatg caacaggctg gagtcctggg tgagcttcca
     2161 ttttgaagaa aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc
     2221 aaaataacgt tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat
     2281 taatactgtc gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc
     2341 accgccgggt acggtaaact ccatctgccg catcgtgccc ggcagacgct gggcggcctc
     2401 ttcataggcg cggtttgata aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg
     2461 aggataggcg gcaatgttgt acaacgtagc cgcatgcagc cagtgtcgac cactgagttg
     2521 cggatcttct tccgcacagg ctttttgttg ccacaccatc gcctgggtgg cccactcata
     2581 aatccagttg ccaccacggt agccaatcac ggtatcgtat aaatcatcat cggtacgttc
     2641 agcatcgctc atcacaatac gtgcctggac gtcgaggatt tcacgtgggt caatgccgcg
     2701 ccagatccac atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag
     2761 agccgactgc acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc
     2821 aggatgttta aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc
     2881 agcctccaga atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc
     2941 ccggcagtgc caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc
     3001 ccatatccca cggctgttcg atccaggtat cttgcgggat atcaacaaca tagtcatcaa
     3061 ccagcggacg accagccggt tttgcgaaga tagtcacaaa atgtgctttt ggatacattt
     3121 cacgaatcgc aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt
     3181 cgccatcgcc ttctgcgcgt tttagcactt taagctcgcg ctggttgtcg tgatcgtagc
     3241 tggaaataca aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg
     3301 gtaccagacc gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc
     3361 ggcttgcgag tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc
     3421 tcatgtgaag tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag
     3481 gttgcgcgag attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt
     3541 ctttttacgc actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc
     3601 aagcctgtct tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc
     3661 gggtgatcga caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg
     3721 tgggatattt ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc
     3781 gctgaataca ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta
     3841 ggtaatatcc tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa accggtcgtc
     3901 ttacaggccc acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg
     3961 aaagatccta tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg
     4021 ggtgcagata acggcattgg tatggcgtct gcgctggcgg ttctggctga cgaaaacgtg
     4081 gttcacggcc cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg
     4141 ttcggcttac agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa
     4201 gaaggtgaaa tctacatggg ttgtgcgggg ggtatcgact tcacctccaa cctgcattta
     4261 gatcgtgaag cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa
     4321 ggcggtcact ccggcggtga aatccacgtt ggcctgggta atgccaacaa actactggtg
     4381 cgcttcctgg cgggtcatgc ggaagaactg gatctacgcc ttatcgattt caacggcggc
     4441 acactgcgta acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa
     4501 gtcgacgtcc tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca
     4561 gagaaagaga aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg
     4621 attgcgaaat ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc
     4681 cgcaattccg acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc
     4741 atgactgaca ataacgtaga aattcactgc ctgatccgtt cactgatcga cagcggtaaa
     4801 gactacgtgg tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg
     4861 aaaggcgcat atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt
     4921 gaaacctatc agcgtctgtt caacaaaacg ccgaacatcc agattatcca cgcgggcctg
     4981 gaatgtggtc tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact
     5041 atcaccggtc cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg
     5101 acactgctga ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg
     5161 gatggcgttt aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc
     5221 cctgtaaatg tccgtctcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc
     5281 gatttcagca ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat
     5341 cgtgccagcc gcttgttagc atgctgacta cgctctgact gaaccttcac gctaataccg
     5401 gatgccaaat gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc
     5461 cccgacgaac gcaacgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta
     5521 aaacgcccaa tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga
     5581 caaatccact gaatagtgcc acaccagctt tcgcttaatg cccatgcgtt atcgccatcc
     5641 agagaaatca gcgccgaacg cagcgtgtca gagtagtggc ccgtttctgt ttccagcacc
     5701 gttaccgcga cgtcttgtcg ggcagcttct ttaattagcc tgtccagcgc ctttttcact
     5761 gcgagacaac attcttccgg cccctgcgct gaggagagtt gtagcaagat catccttttt
     5821 tccctccact gtttttgagc gttagcaccg gacgcagccg cgcgacagga ataattaaac
     5881 cggcctgcac cagacattgc accacgcttt cagccgattt ataggcttgt ggcgcttctt
     5941 caaagatgag ttgtttatcg cgacaaatta cccgactgcc aagttcagta cgcgagagtt
     6001 gcgtcgccgt gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc
     6061 ctgccccatg cgccagcgag tggagcgttt tttcgttcgc aacaggctgt acaatccagg
     6121 agtaatcacc acgtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcacctt
     6181 tacgatgcaa ccagccctgt tgatcgccga tttgacacgc gctaacaaag ttatgcgcca
     6241 catccagaac cggacttccc gtggccttaa cttgttgcat tatgcgcaaa gcgatcatct
     6301 ggcgattaat acacgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt
     6361 cgtcactacc ttgaggcaaa ccatgatgcg agaacgaggc aatatgccgc tgtaaaatag
     6421 actgccccac cccccgcgag ccgctatgaa ccaacagttg cagatgttgt gcatccagac
     6481 ctgccagtgc aaacagttca gcgttgatga tttgatcaac ttgttgcagt tcagcgaaat
     6541 gattgccgcc accgatagag ccaagcgagc tgcgccacgg atgctgtgca aatgctgacg
     6601 gcaggttttc ctccagccag ctttcttcag caacgtcatc cagatctgat aatcgctttt
     6661 caaacttatc ggcgttgtat ttgcgagtga gaatatctgt ttgccatagc gccataccgc
     6721 agccgatatc attgccgacc agtgccggat aaaaacgacc aacagagaag aacgctgcgc
     6781 caatcggata gccgcgtccg ggatgtaaat ctggcatccc cactacgcgc tgcatgttgg
     6841 gtaaatttgc cgtggtgtgt aattgttgga tcgctaaact ctcgatccac aggtcatcag
     6901 atgcgatggt aaataccgca tcggataagg gacgaatata attgcccata taccattccg
     6961 taaatagact cgtcatactt caagttgcat gtgttgcgac ttgaattatt gagagtaaca
     7021 attaaaaaat caggaaatgg cagacgaatc cagggcaaga aaaaaagaat taatcctgga
     7081 aaggtctgca aagagtcatt gataaaggat ttgtcatgat ttacctcctt gcagtttgtg
     7141 gaataagaaa acgcgcacat actaggcttg cgttattttc attgcaagct ggatttaatg
     7201 ttgctgttta taacgcatat aaaaattaat aaaccactct ccccgacatt caacgcgttg
     7261 ctgcttaact gtctgaaaac cataacgttc aaaaaagggt tttgcggtta tgcttgcgtc
     7321 caccgtaagt tcggattcag acttaatcaa aggttttaac aaagcgctgg caaccccacg
     7381 gcgggtgtat tcagggtcaa caaataaca
//
LOCUS       DAZFVO020000209         7312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFVO020000209 DAZFVO020000000
VERSION     DAZFVO020000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.210"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..103)
                     /locus_tag="ACHKNB_RS22730"
                     /old_locus_tag="pgaptmp_004546"
     CDS             complement(<1..103)
                     /locus_tag="ACHKNB_RS22730"
                     /old_locus_tag="pgaptmp_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HGU4197928.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLY"
     gene            complement(115..4323)
                     /locus_tag="ACHKNB_RS22735"
                     /old_locus_tag="pgaptmp_004547"
     CDS             complement(115..4323)
                     /locus_tag="ACHKNB_RS22735"
                     /old_locus_tag="pgaptmp_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HGU4197929.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEMTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPG
                     QQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLY
                     QLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGH
                     AGIGGGINAAITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESV
                     GIGAGVDASVMTLACRTWQECWVN"
     gene            complement(4390..6498)
                     /locus_tag="ACHKNB_RS22740"
                     /old_locus_tag="pgaptmp_004548"
     CDS             complement(4390..6498)
                     /locus_tag="ACHKNB_RS22740"
                     /old_locus_tag="pgaptmp_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HGU4197930.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1463 a         2248 c         1863 g         1738 t
ORIGIN      
        1 gatataaacc acgaaagata ttaacttctt gtctagctgg ctctatgtcc actggagaac
       61 aaccacacaa taaaaataaa actaaaaata tatatttttt catatttcct cctgttaatt
      121 gacccagcat tcttgccacg ttctacaagc cagagtcata actgaagcgt caacacctgc
      181 accgatacca acagattctg tcgatgattc tggattatca acatccacta cataagtata
      241 cgcaaaatgc cccccaactc cgccgccaac cgttacacct actgaattgc tgactcccga
      301 tgtaggtaaa gacttggtct cagaatatgt gatagccgca tttatcccac caccaattcc
      361 tgcatgtccg catgctgtag cataaataca aatatcagat aacatttcac ctttcttttt
      421 agcaaacgca acgccaagtc ctgcatgacc acctaatgat attagatctc cactgccagt
      481 aatatcaatt gatgatgttc catatgaatc atcatgccaa acatcatata ataattgata
      541 taaattatac aaacccaaag gatccatcct tattatcgga ttcagtgggt aattatacag
      601 gttccatccc cccgccagcc caatggggtc ttgtgtaata tacctcccct gtagcgggtc
      661 atagtaccgg ttccggttat agtacagccc tgattcctca tcatgctgct gccccggcag
      721 acggtatggc tggtgcaggt aatacgggtt ctcttcatta agctggttgc cccattcatc
      781 atactccccg cgccacgccg tattgccgtc ttcgctaatg agcgccagcg gcaggccccg
      841 gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg tattccggct ccacctgtct
      901 ggccagttgc tccaccgtca gcccgcactg cgcaagccac gcccggcttt cactgctcac
      961 gcggtctgcc cggatttctt cctccagcct gtccagcagc cgcaccagtt cagccggaaa
     1021 taccacgccg tgcccgtcct cgctcccttc ctgctgcagc ttctccgcca ggctgcggcg
     1081 ctgcgctttc tcccgctcgc cgttgtctgt ttcgatgcgg atgagcggcg cgaagctccc
     1141 cggctgatat accgtctgga tacgcgtggt gtcggtctgt accgtcgtca gccggtcgcc
     1201 gtcccacccg taccacgtca tctccggttt acgcgacagc gacatccacc ccgtcaggtc
     1261 acgctcccgc cgccataccc gtttcgccat tcgccgtccc agcgggtcgt agaggtagcg
     1321 gctctcgacc agtggctcgc catgctgtat ccgcgtgtag aacaccagac ggtgctggct
     1381 gtcgtagtgg tagtggtggg tccgctcgtc gtccgtccgg ataacacccg ccgggataag
     1441 gtccgtcttc tccgtcagtc tgccgtattc atcatagtgg taaacatagt gcgcatcctc
     1501 tgcgatgcgg ttatccggcc acgccgtgag ggtactgtcc gggtgcagct ccgggtccgg
     1561 caggcggttt cccgccgggt cggtggcata cgggatgcgg atgtccaggt ccggtgcgag
     1621 ggtacgcacg ctctccagcc tgcccgtggc gctgtagccg tattcccgcg tctgtcgcgg
     1681 gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt catacaccaa
     1741 gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg tcagtttata
     1801 tgcggcatta ctgcctgcca tgctgccaaa gctgcgcacc gtctcccggt gcatgcggtc
     1861 gcgcgtgtac tccagcagcg gcgtgtcgcc aagcttcatg cccgccagat aaccgctgcc
     1921 gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg ccagcccctg
     1981 ttcgttgtag gcgtgtgttg tctcatgctg ccacagcagt tcccccgtct ccgggttctc
     2041 caccgtctgc cgttcgccgg tcaggcggcc cttatcgtcg tagccgtagt ggacagcgac
     2101 acggtggcct tcgctcaggt ggctgatgtc tgtcagccag ccgtggtcat catactgcca
     2161 ctgctctgcc ggttcgccgt tcaccgtgcg gtgagtgata cggtccgatt catcgtagta
     2221 ccagagggtg acaagcccct catcttcact ctgcgtgagt tttccggtca ggtcataatg
     2281 ataacgttgc gtccgcccgt caaagccgcc ctgctgtacc aaccggtcca gcgcatcgta
     2341 gctgaagtcg ctgtggctgc cgttctcgtt agtcagactg atgacacgtc cggcagcatc
     2401 gtactccata ctgcgcgtca gcccgccctg cgtggtgctg accgcctttc cccacgcatc
     2461 gtactgtgtc tcgctccggt tgccgtccgg ggtgataacg gcagtcaggt cgcctgcggc
     2521 gttgtactca tactgcgttt cacggccctg tgcgtctttc accgaggtta accggccacg
     2581 gttgtcatag tggcggtaaa ggctgatacc ttcctcgcgg tgaaccgccg tcatctggcc
     2641 gaagcggtcg tattcatagc gggtctggta gcccgagcag tcagtgaacg ccagcaactg
     2701 cccgtagcgg ctccacgtca tctgccgggt gctgcccgtc gcatccgttg tcgtcgccgg
     2761 taactcactg tgcgcgtcat cgtagcggta gcgtactgtc tccccgctgc gcgatgtctc
     2821 cgataccagc ctgcccggtt catcatattc ccggcggctc tccagcccgt ccgggtacac
     2881 caccgccgtc agctggttcc cgtcgttata gtaaaatttc gtctcccgcc cgtccggtgt
     2941 ggtgatgtcc gtgatatcgc cggacaccac attcagaccg tactctgtcc gccgtcccgc
     3001 cgcgtccgtc tgcgccgtga gccttcctgc cgcgtcatac ccgctgtgcg tgacgctgcc
     3061 gtccgccagt tcttttttca ccacccgctt cagcccggcc ccgccttctg tatgcagcac
     3121 ctcacgccgg ttcaggctgt cggtgacggt gatgcggtcc tgctcatact ggtagcggta
     3181 actcaggcct gccgggttca gctgctccac cacccgcccg gtatcgtcgt agcggtagcg
     3241 catctccggc cttcccgcgt aacggtgcgc caccatccgg cccgggtgct gcgcgtcata
     3301 cgtgaaagcg cgcacctgcg tattgctgcg gtcatatacc gccagcagtt caccggcttc
     3361 cgtatacgtg taccgcgcca gtggcgcacc gggcaggctt tccgggtatg ccgggtcgtg
     3421 catcagccac accgccgaaa gacggatgcc cctgtcgggg ccgtattcgg taccgggcag
     3481 tgtgtcgggg aacggtgagg ctgagagagg gcgggaactg tcagaagaag atagcgaaga
     3541 ggtgcgggcc tcttccgccc gctgtgcctg tgtggtcagt accagacgga actcacgccc
     3601 ggcaccgtcc gtcacgccgg tgatttcccc ggccaggtcg cctgcggcct cacgccggta
     3661 cgtcagcgtc cgcccgaaac ggtctgccag cccggtaagc acccggtacg gcggcagcgg
     3721 cgctggcagt acgtcctcag cacccggcac ccgctctgac caccccagta tccaccacgg
     3781 cccctgtgcg ctgttggtcg ccaggtaaag atgcgggctt aaccggatat ccggcggcag
     3841 cgccccccac agccgcgcca gcgtgtggcc gtccggctgc gctgccttac caccgcgcac
     3901 cagccacatt gactcgctgc ggctgtacac cgcctccccc ggcagcagcg gctcaaagtg
     3961 aatgctccgc ccgccgttgt cgttgagtac cagtgcatca tcgcgtagct gtaagcggat
     4021 atcagaaggc gctttccagc cggggccaaa cactcccacc ggcgcgggtg tcctggtccg
     4081 gtagctgctg taggtgcggg agagaatgaa cggcagcggg ccgggcagcg caaggtccgt
     4141 ctcgccgggc agcaccttcg cccccagcag cggatttacc gggttgcccg aagtcatccc
     4201 gcccggacac accgagcacg ccacgccggt gggcgcgcca attcttacac ctgccgaacc
     4261 ctggacaata ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct
     4321 catgattcaa ttccttcttc tgtttatttt taatgcttgt ttgttactga ataccgaaca
     4381 ggtacgggat cagtactctc tcatatccgg cgtatcctgc ttttcatcag gggtcttcgg
     4441 tggggatttc gaggctgcac agttccagtt aatcaacagt gagtcaccat taacctgctc
     4501 cttaccctgc aaatgaattt ttgtgccgtt gagaaaaatt tgtccgtcct tcgtcagcac
     4561 cagccttgcc ttaccacagc agagctccag atgctcaccg gcggagtaaa tcgcggtctg
     4621 cccggtatca tcctttttcg ttttaccaac ggtgaaggta acgttatttc cgactttgac
     4681 gtcataaccc agcccgaccc tcaacgattt atgcaaaccg atctgcgagg actgcatcag
     4741 agccaccgaa gtgttcataa tgccacctac cgtcgtctgg tacgccacgc ccacggtcag
     4801 cgcacgaaca agtccaatcg tttccacctg aacagaaccc acgttaatga tctggttact
     4861 gcccaccgtc tctatctggt taacgcccac cgtctgtatc tgattgttgg taaccgcaat
     4921 catctgattg ttgccaatgg tctcagcatg gttgtttatc acatccgtgg tgcggttatt
     4981 cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt tccctgtcgc
     5041 atcgtcaaac ttcagttcat taaacccgct gcccttatag gttttcgaac gaatggtcat
     5101 ctgcgtcttc gtccccggca ggctgccggg tgtgcggttt tcctggtggt aggtacgccc
     5161 cataataatc ggctggtccg gatcgccgtt gaggaagtcc acaatcacct cctgacccac
     5221 acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca cacggatcca
     5281 gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca cccgcacgcg
     5341 accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag cgctctgcgg
     5401 gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg ccgggatcac
     5461 cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg cctgtggctg
     5521 ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     5581 ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca ttccccgcgc
     5641 ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct gcccgtgggc
     5701 acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt aatcctggta
     5761 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtctttggt
     5821 caccacggaa gaagggcgga tttgtgcact gtagcggaac tggctgatgc agagggtgct
     5881 cacctcggta cgggtgttcg gattccaggg gatctcaaag gactccggca gataacgcac
     5941 ggtgtcgcac aggaccaggc tctggtcggt acttttttgc gcgtgctcct cataaaagaa
     6001 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcggtttcgc cgtactggac
     6061 acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccactcgg tcacaccgtt
     6121 ttccttcagg atcgtagcga ggatgctttc gatgtcctca ttctggaaga tacggaagtt
     6181 ctggcgcagc cctgtgcgcc acagcggcgg gcacaccttc atgctgtaca gcatctggtt
     6241 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacgtc
     6301 atcgccctgc cataccgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
     6361 aagaaactgc tgactgacca gagaaaggtc gagggaaaaa agtgaagaga gtgactgggt
     6421 gagatgaaag gaaaccaccg caaacgcgtc cggtggcagg ccgtccactt caagtgtgaa
     6481 acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc acatattgca
     6541 tgtacaacat gtcgggcgga tgagataagc gagtcgacat ctggcgttac cggggaatat
     6601 cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag agaaacgggt
     6661 gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac gttattcacg
     6721 acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca aaccaatatt
     6781 cataatgtct ttttattcac aacaataaat aattcaataa caatcattga aatgaatgat
     6841 gatttgtatt aggaatatac gctaccgccc caccctgata acatgatctg cacattgcca
     6901 tcttaacgtc gttaaacact ggcttaatag tggatatttc aaattaggaa tttattaaat
     6961 tcacctgatg aatatcataa gcaaatagca tagaaaataa tgacaattcc cccgagggga
     7021 ccatttccaa agcactgcac caccaccagg tgcccggatg atcttgcttc agtggtaaga
     7081 gtacctgatt cattgttccc aaaatgatgt tttccccggc cccgccaata atgtgcagct
     7141 ctcacgtgat aatctattga ttaggttata ttaagaatca atatttaaaa tacatccatt
     7201 ttcttgaata aatcaacatg tttatttatc aattgctgtt ttagctctga ctcatcaagc
     7261 tctccatatt tgtcctacag ataattgcga ataaatacag agaacgccat tt
//
LOCUS       DAZFVO020000210         7296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFVO020000210 DAZFVO020000000
VERSION     DAZFVO020000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.211"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(337..1473)
                     /locus_tag="ACHKNB_RS22745"
                     /old_locus_tag="pgaptmp_004549"
     CDS             complement(337..1473)
                     /locus_tag="ACHKNB_RS22745"
                     /old_locus_tag="pgaptmp_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4197931.1"
                     /translation="MDLLIVLTYVALAWTVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1476..1838)
                     /locus_tag="ACHKNB_RS22750"
                     /old_locus_tag="pgaptmp_004550"
     CDS             complement(1476..1838)
                     /locus_tag="ACHKNB_RS22750"
                     /old_locus_tag="pgaptmp_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HGU4197932.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2375..4288
                     /gene="mtlA"
                     /locus_tag="ACHKNB_RS22755"
                     /old_locus_tag="pgaptmp_004551"
     CDS             2375..4288
                     /gene="mtlA"
                     /locus_tag="ACHKNB_RS22755"
                     /old_locus_tag="pgaptmp_004551"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HGU4197933.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4518..5666
                     /gene="mtlD"
                     /locus_tag="ACHKNB_RS22760"
                     /old_locus_tag="pgaptmp_004552"
     CDS             4518..5666
                     /gene="mtlD"
                     /locus_tag="ACHKNB_RS22760"
                     /old_locus_tag="pgaptmp_004552"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGU4197934.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5666..6253
                     /gene="mtlR"
                     /locus_tag="ACHKNB_RS22765"
                     /old_locus_tag="pgaptmp_004553"
     CDS             5666..6253
                     /gene="mtlR"
                     /locus_tag="ACHKNB_RS22765"
                     /old_locus_tag="pgaptmp_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HGU4197935.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6265..6474)
                     /gene="yibT"
                     /locus_tag="ACHKNB_RS22770"
                     /old_locus_tag="pgaptmp_004554"
     CDS             complement(6265..6474)
                     /gene="yibT"
                     /locus_tag="ACHKNB_RS22770"
                     /old_locus_tag="pgaptmp_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HGU4197936.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6759..7121
                     /locus_tag="ACHKNB_RS22775"
                     /old_locus_tag="pgaptmp_004555"
     CDS             6759..7121
                     /locus_tag="ACHKNB_RS22775"
                     /old_locus_tag="pgaptmp_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HGU4197937.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1850 a         1832 c         1849 g         1765 t
ORIGIN      
        1 accctacatt gtgatctata gattaaatat tttttaaatg tgactgtcat ccttcagata
       61 ataaagccat catcgcaata gatggaaaag cactcctgct cttttttgat tagagccgtc
      121 gcaggaggaa caactcatat catcattgcg atctcgacga tgtatagtct catcctgcgg
      181 cagaacaaga cggataaaaa atccaatgag agcacactca tccagaactt attaacataa
      241 tgaatattaa agaaaaatca tcgcagctaa tgaagtaata tgcaaaaata attagtcctt
      301 acaaggagca aagttccata atctcccccc tccccctcaa tgatccaaat aaagataatg
      361 catccagctg gtcattctta gcagcacttt ccgcatcacc gaaacatggc tgaaatggtc
      421 ggagtaaacc gccacctggg cgtagatgcc gtcgggtaag gcatcgatat catcgttagg
      481 gtccagttca atggttccca gcacaccgtc cgtgccgggc acgaccgtta atgattgcaa
      541 taccccctgc gcctgataag aaccgcctgg cacgacaggt aaaatactag tcagtttgcc
      601 gtgaaacacc tgcccaggta gcgcgttaaa caccacttcc gcatcatcgc cgggtttcag
      661 acgtaacagc gagttttgcc gaaattgggc gacaatttgc cgtttttgct cggggatgaa
      721 gaccatcacc ggacgcagcg gcaaggcagc tgcgtatgta cctgggcgga tcagtacctg
      781 agtaacgtag ccattgctcg gcgcgcgaat gacagtctgc tcaaggttat attttgcttc
      841 agtaagttgc gctcttaagc tcacaatctg cgattgctcg ccgttaacca tactgtcgag
      901 ctggctctgg atctgcgcct gctccgccac cgagcctttc accagcgcat cctgcgcgag
      961 gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac ggattcaccg ccgcctggct
     1021 gcctttcaag taacgttgat aatttttaaa cagacggtcg cgctccgctg aaacctgggt
     1081 ggtgttggcc tgcgcttcag tgagctgcgc acgcagcgtc tttatattat gcgtcgccgt
     1141 catcaggtca gcctgaagtc tgtcaactcg cgcctggtaa cgaaccgggt cgagcttaaa
     1201 aagcacctcg cccttttgaa taagctgatt attcttgtca gtgacttcag taacaattcc
     1261 cgtcacctgt ggcgtgatag ggatcgctat cactgccttt tgcgcggtaa aagtgtaagg
     1321 gtggttgtag ttcatcaaca aaattaaacc actcaccaga aacacgcctc ccagcgccgc
     1381 cgtcgccagc gtccactgat ttaccggaat gcggaagatt ttaaagaccg tccacgccag
     1441 cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa cgtggaatag
     1501 tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc agaaagtgag
     1561 gcgatttccg ggtcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca
     1621 cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta
     1681 aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg
     1741 gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg
     1801 aatatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta
     1861 tcagtgttgt ttttgggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc
     1921 cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta
     1981 gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc
     2041 gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg
     2101 tgacactact cacatttaaa tgccattttt agcgaaaatc gccgtcttgt tgctttttta
     2161 cacaagcgtt ttgtgatgag tatcacgtca attacctctc tgccccctat atttatgtga
     2221 ctgatatcac acaaaaggcc gtcgactgga cagttaaccg attcagtgcc agatttcgca
     2281 gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc
     2341 ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt
     2401 gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc
     2461 gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc
     2521 gaagctggtc gggccgatga tcacttatct cctgccgctg ctgatcggtt ataccggtgg
     2581 taagctggta ggcggcgaac gtggcggcgt agtcggtgcc atcactacca tgggcgttat
     2641 cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg
     2701 gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct
     2761 ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gccattctgg catttctcgg
     2821 cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt
     2881 tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct
     2941 caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact
     3001 gggtaaatcc atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct
     3061 ggcgtacatg ttctttggtc gtggtagtgc taaacagtct gcgggcggtg cggcaatcat
     3121 ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct
     3181 gatcctggcc gttatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg
     3241 tggtctggtt tctccagcat ctccggggtc tatccttgcg gtactggcga tgacgccaaa
     3301 aggtgcttac ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt
     3361 ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac
     3421 tcgtcgtatg caggacatga aagctgagtc taaaggcgca tctccgctgt ctgctggcga
     3481 tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg
     3541 ttccagtgcg atgggcgcag gcgttctgcg taagaaaatt caggatgcag gtctgtcgca
     3601 gatttctgtt actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac
     3661 tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct
     3721 gaccaacttc ctcgacagcg gcctgtacac cagcctgacc gaacgtctgg ttgctgccca
     3781 acgccacacg gcaaacgaag agaaagtaaa agacagcctg aaagacagct ttgacgattc
     3841 cagtgctaac ctgttcaagc taggcgcgga gaacatcttc ctcggtcgca aagcggcaac
     3901 caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt acgttgagcc
     3961 ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc tgggtgagtc
     4021 tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt
     4081 gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct
     4141 ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca gcctgaccaa
     4201 tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg tggatgaagt
     4261 gctggaactg ttggcaggtc gtaagtaatc caatcccacc ctctccacat ggagaaggtg
     4321 gggttaattg cctgatgcgc tacgcttatc aggcctacag gatgcatcac gatttgttga
     4381 atttgcacgt tcttgtaggc cggataaggc gcttacgccg catccggcgc tgcccctctc
     4441 ctcacggaga gggtttgggt gagggaaaag cctcacccca gccctctcgg gtaaaaacat
     4501 tgatgaaggt taatactatg aaagcattac attttggcgc aggtaatatc ggtcgtggct
     4561 ttatcggtaa actgctggca gacgcgggta tccaactgac gtttgccgat gtcaatcagg
     4621 tggtacttga tgccctgaat gcccgtcata gctatcaggt tcatgtggtt ggtgaaaccg
     4681 agcaggtaga taccgtttcc ggcgtcaatg ctgtcagcag cattggtgat gatgtcgttg
     4741 atctgattgc tcaggtcgat ttagtcacta ccgccgttgg cccggttgtg ctggaacgta
     4801 ttgctccggc tatcgccaaa gggctggtga aacgcaaaga acaaggtaat gaatccccgt
     4861 tgaacatcat cgcctgtgaa aacatggtac gcggtaccac gcagctgaaa ggccatgtga
     4921 tgaacgccct gccggaagac gccaaagcgt gggtagaaga acacgttggc tttgtcgatt
     4981 ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg ctggaagtga
     5041 cggtagaaac tttcagcgaa tggattgtcg ataaaacgca gttcaaaggc gcactgccga
     5101 acatcccagg catggagtta accgacaacc tgatggcatt tgtcgaacgt aaactcttca
     5161 ccctgaacac gggtcatgct ataaccgcgt acctcggaaa actggccggt catcagacca
     5221 ttcgtgacgc tattctcgac gagaaaatcc gcgcggtggt aaaaggtgcg atggaagaaa
     5281 gtggtgcggt actgatcaag cgctacggct ttgacgcaga caaacatgcg gcgtacatcc
     5341 agaaaatcct cggtcgtttt gagaacccgt atctgaaaga tgatgtagag cgcgtaggcc
     5401 gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccattg ctcggtacgc
     5461 tggaatacag ccttccgcac aagaatctga ttcaggggat tgctggtgca atgcacttcc
     5521 gcagtgaaga tgatccgcag gctcaggaac tggcagcact gatcgctgac aaaggtccgc
     5581 aggcggcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta tccgaggcgg
     5641 taaccgctta taaagcaatg caataatggt ggaccaggcg caggacaccc tgcgcccgaa
     5701 taacagattg tcagatatgc aggcaacaat ggaacaaacc caggcctttg aaaaccgtgt
     5761 gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg ccgtcgagct
     5821 tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg attacgcggt
     5881 gaagtatgct gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc tttctgtgcg
     5941 tttaaaactc atttacgggt tgggcgtcat taaccgccag gaatacgaag atgcggagct
     6001 gctgatggca ttgcgtgaag aactaaatca cgacggcaac gagtacgcct ttaccgacga
     6061 cgagatcctt ggaccctttg gtgaactgca ctgcgtggcg gcgttaccac cgccgccaca
     6121 gtttgaacca gcagactcca gtttgtatgc aatgcaaatt cagcgctatc aacaggctgt
     6181 gcgatcaaca atggtccttt cactgactga gctgatttcc agaatcagct taaaaaaagc
     6241 ctttcaaaag taagcaacgt ctgcttactg cccctctacc tgcttcggcc gataaagccg
     6301 acgataatac tccagacgtt gtagatataa cggcacgctt tcatcgggta ttccggacgg
     6361 aatcgcgtta cggggaggaa gttttttcag atactcccgg aacgcctggc ttgatgccat
     6421 gaaatctacg gctttatcga taagaagcgg aacgttttca cctaatttgc ccatgatgat
     6481 atctccgtat taccccgcgc cgggaatgcg cggccgccaa ttttagttta ggttcccggt
     6541 aaagatgaca ttaaggaaaa cgtgctgaat cctcaataat catccgctgc tgtttattat
     6601 cttttttcta tagatttctt aattaatcaa taaattgcgt agtttttaag catgttaatg
     6661 attacgttac agaactattg caatgtcatc agttgtgaac aaagcacctg gtcgcgcata
     6721 ctaggggcta taaatttatc tttatcagaa gccatcacat gaaagaagtc gaaaaaaacg
     6781 aaatcaaacg tctcagcgat cgtctggacg ccatccgcca ccagcaggcc gatctgtcgc
     6841 tggttgaagc cgcagacaaa tatgccgagc tggaaaaaga gaaagccacg ctggaagcag
     6901 aaattgctcg cctgcgtgaa gttcatagcc aaaaactgag taaagaagca cagaagctga
     6961 tgaagatgcc gtttcagcgc gcaattacca aaaaagagca ggctgatatg ggcaagctga
     7021 agaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggc cgtgaaatgg
     7081 gcctgcagga gatgactgga ttctctaaga ctgcgtttta agcgcctcag acgactggtt
     7141 cgatcatatc aacccgcatc tggcttgcct tagctggtca atgcggggac tctcctgtct
     7201 actcgcctaa ttaccaaaca attcactccc tttacgtcga tttaccgctg gttgatattc
     7261 tgaaaatgca tttttaccga aatagccacc tataaa
//
LOCUS       DAZFVO020000211         7286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFVO020000211 DAZFVO020000000
VERSION     DAZFVO020000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.212"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(159..2045)
                     /gene="prpE"
                     /locus_tag="ACHKNB_RS22780"
                     /old_locus_tag="pgaptmp_004556"
     CDS             complement(159..2045)
                     /gene="prpE"
                     /locus_tag="ACHKNB_RS22780"
                     /old_locus_tag="pgaptmp_004556"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HGU4197938.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(2085..3536)
                     /gene="prpD"
                     /locus_tag="ACHKNB_RS22785"
                     /old_locus_tag="pgaptmp_004557"
     CDS             complement(2085..3536)
                     /gene="prpD"
                     /locus_tag="ACHKNB_RS22785"
                     /old_locus_tag="pgaptmp_004557"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HGU4197939.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(3570..4739)
                     /gene="prpC"
                     /locus_tag="ACHKNB_RS22790"
                     /old_locus_tag="pgaptmp_004558"
     CDS             complement(3570..4739)
                     /gene="prpC"
                     /locus_tag="ACHKNB_RS22790"
                     /old_locus_tag="pgaptmp_004558"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HGU4197940.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4784..5674)
                     /gene="prpB"
                     /locus_tag="ACHKNB_RS22795"
                     /old_locus_tag="pgaptmp_004559"
     CDS             complement(4784..5674)
                     /gene="prpB"
                     /locus_tag="ACHKNB_RS22795"
                     /old_locus_tag="pgaptmp_004559"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HGU4197941.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5913..>7286
                     /gene="prpR"
                     /locus_tag="ACHKNB_RS22800"
                     /old_locus_tag="pgaptmp_004560"
     CDS             5913..>7286
                     /gene="prpR"
                     /locus_tag="ACHKNB_RS22800"
                     /old_locus_tag="pgaptmp_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HGU4197942.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERL"
BASE COUNT         1581 a         2063 c         2006 g         1636 t
ORIGIN      
        1 cagcgtcgca tcaggcatcg gcgcggggtg tcggatgcga cgcgagcgcc ttatccgacc
       61 tacggttcag gcctgtaggc ataataagac gcggtaagcg tcgcatcagg catcggcgca
      121 tggtgtcgga tgcgccacgc gacgcatccg acaatacctt actcttccat cgcctggcgg
      181 atctgatcca acgacgctgg atcatcaatg gtcgtcagat ctccaggatc gcgtccttcg
      241 caaatcgcct ggatcgtgcg gcgcagcatt tttccggatc gcgtttttgg caattgcgag
      301 acaaaccaga cgtgcgccgg gcggccaaag ttgccaatct ggctgtccac cagcgccata
      361 atcgccttct cttgcgaatg cgccacatca cgatcttcca gactgtcgct ctctttcgga
      421 atgacaaacg ctaccgccac ctgccctttc agcgcatctt tcaccccaac caccgccact
      481 tcggcaacgc ccggatgact ggaaatactc tcttcaatct cgcgcgtgcc cagccgatgc
      541 ccggcaacgt taatcacatc gtcagtgcgc ccgagaataa agtgataacc gtcagcatcg
      601 cggatgcccc agtcaaaggt ggcgtacacc ggacgggaaa acagcgacca gtacgtcttc
      661 acaaagcggt cgtcatcgcc ccagatggtc tgaatacacc ccggcggcag cggcccttcc
      721 accaccagca tccctttctc gttgacgcca cacggttcgc cggtgacttc attaagcaac
      781 tgcacgttat agccatacat cggcacgccg gggcttccca gacgcgtcgg cctgtcgtcc
      841 agaccgcgag caatcgccat aatcggccag ccggattcgg tctgccagta gttgtcgatg
      901 accggcacat ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag cggttctcca
      961 gccagataga gcacttccag cgacgagaga tcgtgtttgc gaatttcagc ggtagggaat
     1021 tttttcagca cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg atatttctcg
     1081 acgattttcc accacacgcc gcagtccggc caggtcggta atccttcgta aacgatagtc
     1141 gccatccccg ccagcagcgg cgcgtagacg atatacgaat gccccaccac ccagccgata
     1201 tccgaagcac aaaagaacac accgcccgct ttgccgccaa aaatggtgtc catcgaggtc
     1261 gccagcgcca ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt gccggtcgtg
     1321 ccggaggtgt agagaatgca ggaggtttcg ttggattcca gccacgccac cggtacccgc
     1381 gcgccgatgt gttgatggcg caacgacgcg aaatcgacat cccgcccgct gacgcgcgcc
     1441 attttcgcca gcccgcgatc caccagcaaa acatggcgcg gctggtgctg cgcctgactt
     1501 atcgcatcgt cgagcaattt tttataggga atgattttgc caccgcgcgc tccggcatca
     1561 gccgagacaa tcagcaccgg tttagcatca tcaattcgcg ccgccacgct gtgcgaggca
     1621 aatccaccaa acaccaccga gtgaatagcg ccaatgcgcg cgcaggccag cagggtaata
     1681 tgcgcttcgg caatcatcgg catatacacc agcacccgat cgccacgctg cacgcccagc
     1741 gagcgcagca ttgacgccac cgcgtttact tcgtcatgaa gctggcgaaa ggtaaaggtg
     1801 cgctcttctt ctgtttccga agagacggca atcagcgcca gcgcctctgg ctgtttctcc
     1861 agccagcgat cgatagcgtt gtgacacaag ttggttcggc cttcacaaaa ccaacgggca
     1921 aacggcggat tgctgtgatc gagcgtttgc gtaaagggcg tctgccagtc aatacgccgg
     1981 gcctgctcgg cccagaactg ctccggttcg ttaatcgaac gctgataaaa ttcgctaaaa
     2041 gacataatgc tctcctgttg aacttacgcc ttaccgccgt ttacttaaat gacgtacagg
     2101 tcgagatact cattgaccgg catctgttcc aggcgagctc tgtcgagaga aacctccaga
     2161 atgcgctgct gctggcgagt cgggaactgg cgcgcgagat tgattttgaa tttatccacc
     2221 agcttcggaa tgccatcctg acggcggcga gcatgaccaa tggggtactc caccaccact
     2281 tcttcaaagc gcgtgccgtc ggtgaactca agagtaatgg cattggcgat ggcgcgtttt
     2341 tccgggtcgt ggtagtcagc ggtaaacgtc ggatcttcaa agcaattgat cttctcgcgc
     2401 agggcgtcaa tgcgtttatc ttgcgcaacg ttgtcctcgt aatctgccgc cgttaagcgc
     2461 ccgaacaaca gcgggatcgc caccatgtat tgaatgcagt ggtcgcggtc agccgggtta
     2521 ttgagcggcc cttttttgtc gatgatgcga atacaggctt cgtgggtgcg aatggtcact
     2581 ttttcgatat ccgccgccgt tttgcctgct gcttgcatct gttcatagag cgtcatcgcc
     2641 gcttcaactg ccgtctggga gtggaactcc gccgggaagg agattttgaa cagcacattt
     2701 tccatgacgt aagaaccgta cgggcgctgg aagcggaacg attcaccttt gaaggagacg
     2761 tcgtagaagc cccacaccgg cgcagtcagg gctgacgggt aacccatttc gcccgttttc
     2821 gccatcagtg ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca ggatttacgc
     2881 gtgccggtgt tcggcgcatg gcgataggtg cgcagcgact gaccgtccac ccacgccagc
     2941 gaaacggcgt tgagaatttc ctcgcgggtc aggccaagca tttcggcgac cacggcggtg
     3001 gaagccactt tcactaacag aacgtggtcg aggccaacgc ggttaaagga gttttccagc
     3061 gcgatgcagc cctgaatttc atgggctttg atcattccgg tcagcacctg tttcatggtc
     3121 aacggcgctt tgccgctggc gaccgcgttg cgcgaaagcc agtctgccgt tgccagaatg
     3181 ccgccgaggt tgtcggaagg atgcccccac tccgccgcca gccaggtatc gttgaaatcg
     3241 agccaacgga tcatcgcgcc gatgttaaat gccgcctgga cggggtcgag ctggaactga
     3301 gttccgggga cgcgcacgcc gttaggtacg acggtgccgg ggacaattgg ccccagcagt
     3361 tttttacagg ctgggtattc gagagcttca agaccgcagc cgagcgtgtc gagcaggcag
     3421 taatgtgcgg tgtcgtaggc tactttggag ctgatttcgt agttcatgac gtaatcgacg
     3481 atatcaacga tttcacgatc aaattccggg cggatgttgt tgatttgagc tgacattggg
     3541 tacgtttcct tattgttatt cgtagaggtt tactggcgct tatccagcgc gacaaactgg
     3601 cggtcttccg gtccaacata attggcggaa ggacggataa ttttgttgtc ctgacgttgt
     3661 tcgataatgt gcgccgccca gccggtgacg cgggcgataa caaacagtgg tgtgaacatc
     3721 tcggtgggaa cgcccatcat gttgtaggaa acagcagaga accagtcgag attggggaac
     3781 atctttttgc tctcccacat caccgtttcc aggcgatcgg cgatgttgta catcttcagc
     3841 gagccgcctt cctgcgagag ctgcttcgcc acacgtttaa tcacctggtg gcgcgggtca
     3901 gcgatggtgt aaaccggatg accaaaacca atgaccactt ctttgttttc cacgcgcttg
     3961 cggatatctg cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc cagcgacact
     4021 tcattcgccc cgccgtgttt tggcccgcgc agtgcgccaa tcgcgccaat aatcgcggaa
     4081 tacatatcag agccggtgcc cgcaatcacc cgactggtaa aggtggaggc gttaaactcg
     4141 tgttcggcgt acagcaccag cgagatatgc atcgcctttt cccagctttg cgatggcttt
     4201 tcgccgtgca gcagatgcag gaagtgaccg ccgatggagt cgtcatcggt ttccggttgg
     4261 atgcgttcgc cgttgtggct gtagtgatac caataaagga gaatcgagct aagcgacgcc
     4321 agcagtttgt cggcaatatc ccgcgcgcca gagacggtat gcccctcttt ttctggcagc
     4381 gtgcagccga gcgcggaaac accggtgcgc ataacatcca tcgggtgtga cgccgccggt
     4441 aaggcttcca gcacggtacg cacgttagcc ggtaaaccac gcagggcttt cagtttcgtt
     4501 ttgtaagcgg cgagttcgtc acgggtcggc agtttgccgt ggatcagcag atgagccact
     4561 tcttcaaatt cgcaatgttc cgccagatca agaatatcgt agccacggta atgcaggtca
     4621 ttgccacttt tacccacggt gcagagcgcc gtattgcccg ccggaacgcc ggaaagtgcc
     4681 accgattttt tcggtttaat gacatgggta ctgttttgca ggatcgttgt gtcgctcata
     4741 ttgtcctcgt catttgtagg gtcgagatat ttttcgttgt gttttatttc acctggctac
     4801 gggcaaacag gtcgtcgagc ttctcttcgt actggtagta gttgatgctt tcgtacagtt
     4861 cgttgcgggt ctgcatggtg tcgatgacgc ttttctgcgt gccttcctgg cgcaggacgt
     4921 tgtagacatg ttcagcggcg cggttcatgg cgcggaacgc tgaaagcggg tacagcgcca
     4981 ttgcgacatg ggcgctgcgt aattcgtcgg tggtaaacag cggcgtggca ccaaattcgg
     5041 taatgttggc gaggattggc acctgaaccg catcggcaaa ctggcggtac atggcgagtt
     5101 cggtaatcgc ctccgggaac aacatctcgg cacccgcttc aacataggcc tgtgcacgct
     5161 cgatcgccgc atccagcccc tctaccgcca gcgcatcggt gcgcgccatg atcacaaaat
     5221 caggatcggt tttcgcatcc accgccgcgc ggatccgatc caccatctcc tctttcgaga
     5281 cgatcgcttt gttcgggcga tgaccgcagc gtttcgcacc aacctgatct tcaatatgca
     5341 atcctgccgc accggcttta atcatcgatt tcaccgtacg cgccacgtta aaggccgaag
     5401 aaccaaaacc gatatccgca tccaccagca gcggcagcga acaaacgtcg gtgatacggc
     5461 gaatgtcggt cagcacatca tcaagggtag aaataccgag atcgggcagc cccagcgaac
     5521 ctgccgccac gccaccgccg gagagataaa ttgcctgata tccggcacgc tgcgccaaca
     5581 gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg attttcttta gacagtgcag
     5641 cgcgaaacgc tttacctgga gagtgtagag acatagccca tcctttgtta tcaacttgtt
     5701 acttgcgttg ataaagacaa agcaaggggt gtgccacaat taactgaata aaattaaatg
     5761 tttaatttca atttgttata aataaagtca tgaactaatc cgcaaatatg cgtttcagtt
     5821 aacgtttcag gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca agactaaacc
     5881 caatattcgg tttcttaact ttgcggtgcg ctatggcaca tccaccacgg cttaatgacg
     5941 acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt cgcgatatca
     6001 gcctcgagtt tgatcacctg gcgaacatta cccctatcca gcttggcttt gaaaaagcag
     6061 tgacctatat ccgcaagaaa ctggcaaacg aacgctgcga cgccgtcatc gccgctggct
     6121 ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt tattttgatt aaaccgagcg
     6181 gctacgatgt gttacaggca ctggcaaaag ccggaaagct cacctcttct atcggcgttg
     6241 tcacttatca ggaaactatt ccggcactgg tggcgtttca aaaaaccttt aatttgcgcc
     6301 tcgatcaacg tagctacatt accgaagaag acgcccgcgg gcagattaac gagctcaaag
     6361 ctaacggcac cgaagcggtg gtcggtgcgg ggctgattac cgatctggca gaagaagccg
     6421 gaatgaccgg aatttttatc tattccgccg tcaccgtgcg ccaggcgttc agcgatgcgc
     6481 tggatatgac gcgcatgtcg ttacgccata acactcacga tgccacccgc aacgccctga
     6541 gaactcgtta cgtgctgggc gatatgctcg gtcaatcacc acagatggaa caagtacggc
     6601 agactatttt gctgtatgcc cgctccagtg cggcggtgtt gattgaaggg gaaacgggga
     6661 cgggcaaaga gctggcggcc caggcgattc atcgggaata ttttgcccgc cacgatgcgc
     6721 gacagggcaa aaagtcgcat ccgtttgttg ccgtcaactg cggggcgata gccgaatcgc
     6781 tgctggaagc ggagctgttt ggctatgagg aaggggcgtt taccggctcg cgacgcggag
     6841 gtcgcgccgg gctgttcgaa attgcccacg gcggtacgct gtttctggat gagattggcg
     6901 aaatgccgct acctttgcag actcgcctgt tacgggtgct ggaagaaaaa gaggttactc
     6961 gcgtcggcgg gcatcagcct gttccggtgg atgtgcgggt cattagcgcc actcactgca
     7021 atctggaaga agatatgcgg caagggcagt ttcgccgtga tctgttttat cggctgagta
     7081 ttttgcgtct gcaattgcca ccactgcgcg agcgggtggc ggatattctt ccgctggcgg
     7141 aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc gcattacgcc
     7201 agggattaca ggcaagcgaa accgtgctgg tgcactacga ctggccaggc aatattcgtg
     7261 aactgcgcaa tatgatggaa cgactg
//
LOCUS       DAZFVO020000212         7238 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFVO020000212 DAZFVO020000000
VERSION     DAZFVO020000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.213"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            219..1592
                     /gene="aroP"
                     /locus_tag="ACHKNB_RS22805"
                     /old_locus_tag="pgaptmp_004561"
     CDS             219..1592
                     /gene="aroP"
                     /locus_tag="ACHKNB_RS22805"
                     /old_locus_tag="pgaptmp_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HGU4197943.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKNB_RS22810"
                     /old_locus_tag="pgaptmp_004562"
     CDS             complement(1635..2489)
                     /gene="ampE"
                     /locus_tag="ACHKNB_RS22810"
                     /old_locus_tag="pgaptmp_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HGU4197944.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKNB_RS22815"
                     /old_locus_tag="pgaptmp_004563"
     CDS             complement(2486..3037)
                     /gene="ampD"
                     /locus_tag="ACHKNB_RS22815"
                     /old_locus_tag="pgaptmp_004563"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HGU4197945.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDVLFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKNB_RS22820"
                     /old_locus_tag="pgaptmp_004564"
     CDS             3125..4018
                     /gene="nadC"
                     /locus_tag="ACHKNB_RS22820"
                     /old_locus_tag="pgaptmp_004564"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGU4197946.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKNB_RS22825"
                     /old_locus_tag="pgaptmp_004565"
     CDS             4221..4661
                     /gene="ppdD"
                     /locus_tag="ACHKNB_RS22825"
                     /old_locus_tag="pgaptmp_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HGU4197947.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKNB_RS22830"
                     /old_locus_tag="pgaptmp_004566"
     CDS             4671..6056
                     /gene="gspE"
                     /locus_tag="ACHKNB_RS22830"
                     /old_locus_tag="pgaptmp_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HGU4197948.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENDCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6046..6966
                     /gene="hofC"
                     /locus_tag="ACHKNB_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /pseudo
     CDS             6046..6966
                     /gene="hofC"
                     /locus_tag="ACHKNB_RS22835"
                     /old_locus_tag="pgaptmp_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7095..>7238)
                     /locus_tag="ACHKNB_RS22840"
                     /old_locus_tag="pgaptmp_004568"
     CDS             complement(7095..>7238)
                     /locus_tag="ACHKNB_RS22840"
                     /old_locus_tag="pgaptmp_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4197949.1"
                     /translation="ASVHKFRQFRYIRFLNKRARKRLNTKLFKVQPYPKPQTVKTGLK
                     EIG"
BASE COUNT         1773 a         1910 c         1843 g         1712 t
ORIGIN      
        1 ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca taaagtgggt
       61 gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgt aacaattcaa
      121 acttctttga tgtaaacaaa ttaatacaac aaacggaatt gcaaacttac acacgcatca
      181 ctgcgtagat caaaaaaaca accaccgcac gaggtttcat gatggaaggt caacagcacg
      241 gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg ctgggtggcg
      301 cgatagggac cgggttattc ctgggtagcg cctccgtaat acagtccgca gggccaggga
      361 ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt cagctgggtg
      421 aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat aaatactggg
      481 gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt ttagttgcca
      541 tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa atccccacct
      601 gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc aacgttaaag
      661 tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga
      721 tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg accgttagca
      781 acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg atgatgatgg
      841 cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca gcagaagctg
      901 ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt
      961 tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc gttaccgccg
     1021 ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg gcgaatgcgc
     1081 tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca
     1141 gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg
     1201 ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg gcgttgtgcg
     1261 tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg ctggtggtat
     1321 ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca
     1381 agcaggaaca aggcgtggta actcacttcc ctgctctgct ttatccgctg ggtaactgga
     1441 tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca ggaatggcga
     1501 tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat ctgtttaaag
     1561 agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc cgtacagggt
     1621 gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat caccaccacg
     1681 accacgaacg aggttttctt cgccattgaa accgctgccc tcggcgtctc caccttatcg
     1741 acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg atactgcgaa
     1801 gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc atgaccgatc
     1861 aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg aagcacggca
     1921 tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca gtattgccat
     1981 gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc cacaatcagc
     2041 cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca
     2101 cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt gagttcgcca
     2161 gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata agcatgataa
     2221 tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca caccagtagc
     2281 gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc
     2341 gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac
     2401 gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt
     2461 aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc acacgaaacc
     2521 gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata tcacaatggc
     2581 ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta accgccgcaa
     2641 gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa
     2701 tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca tgccatgcac
     2761 gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa cagtgagcgg
     2821 agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca atagttccag
     2881 tgaataatac gtcgatccac ggaccgccaa actcgcctgg cggcaggcta atattgtgca
     2941 ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc
     3001 ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt atatgtggtg
     3061 ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta tctggagttt
     3121 taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc tggaacgcat
     3181 taatctcgat atccccggcg cggtagccca ggcgctgcgg gaagatttag gcggaacagt
     3241 cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct ctcatgccac
     3301 ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag aggtgtttat
     3361 tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg atgtcatcaa
     3421 tgccaatcaa tccttgttcg aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg
     3481 cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac gccactatgt
     3541 cgaattgctg gaaggcacca acacgcagtt gttggatacg cgcaaaacct tacccggcct
     3601 gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc gtctggggct
     3661 ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag tgcgccaggc
     3721 ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag tagagaatct
     3781 ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg ataacttcga
     3841 aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc
     3901 tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg actttatctc
     3961 cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt ttcgctaata
     4021 gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca actttcttcg
     4081 taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc ggaatctgct
     4141 ttcccgtttc gttttttgcc cctcgcctgt gctgatggcg tttgccaaag tagcgccaac
     4201 taaatcaagg agcgaaacag atggacaagc aacgcggttt tacacttatc gaactgatgg
     4261 tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat caaaactacc
     4321 tgcgcaaagc cgcactcacc gacatgctac aaacctttgt gccttaccgt accgccgtag
     4381 agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc aatggcattc
     4441 cctcgcctac caccacccgc tatgtttcag ccatgagtgt ggcaaagggc gtggtgtcgc
     4501 tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacaccgggt tgggataacg
     4561 caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc gcattgcatc
     4621 aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca atgaatattc
     4681 cacagctcac tgccctgtgt ctgcgttatc atggagtctt gctggatgcc agcgaagagg
     4741 tggttcatgt tgcggtagtc gatgcacctt cgcatgagct actggacgca ttgcatttcg
     4801 ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa ggtcacgcca
     4861 gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc aaagcagagt
     4921 tgctaactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt catatcgaac
     4981 cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat cctttaccgg
     5041 atgtttcacc ggatgccgga gtcgcattaa ccgccagatt aaaagtgctg ggaaacctgg
     5101 atattgcgga acatcgcctg ccgcaggacg ggcaattcac tgtcgaactg gcaggaaacg
     5161 ccgtctcatt tcgtattgcg accttaccat gtcggggtgg tgaaaaggtg gtattaaggt
     5221 tgttacagca ggtgagccag gcactggatg tcaacacgct tggaatgcag ccgttacaac
     5281 tggcggactt tgctcatgcc ttgcaacaac cacagggact ggtgctggta actggcccta
     5341 caggcagcgg caaaacggtc acgctttata gtgccctgca aacgctgaat accgctgaca
     5401 ttaatatttg tagcgtcgaa gatccggttg agatccccat agccggacta aaccagacgc
     5461 aaatccatcc gcgtgccgga ctcacctttc agggcgtgtt gcgtgcgtta ttgcgccagg
     5521 atcctgacgt catcatgatc ggagagatcc gcgatggcga aacagcagag atcgctatta
     5581 aagcggcgca aactggtcac ctggtgttgt ctaccctaca cactaattcc acctgcgaaa
     5641 cgctggtacg tttacagcaa atgggggtcg cccgctggat gctatcatcg gcgcttacgc
     5701 tggtaatagc ccagcgtctg gtacgcaaac tttgcccaca ttgtcgccgg cagcaagggg
     5761 agcccatcca cattccagac aatgtatggc catcgccgct gccccactgg caggcacccg
     5821 gttgtgtaca ttgctaccac ggtttttatg gtcgtacggc cttatttgaa gttctgccca
     5881 taacgccggt cattcgtcag cttatttccg ctaataccga cgttgaatcg ctggaaacgc
     5941 acgcacgaca ggcgggtatg cgtacgcttt ttgaaaacga ctgcctggcc gtggagcaag
     6001 gcttaaccac ctttgaagag ttaatccgcg tactggggat gccgcatggc gagtaagcaa
     6061 ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc aagatgggat gctatgggca
     6121 gagagccgtg ctttgctgct catggcacta cagcaacaga tggttacccc acttagcctg
     6181 aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata aaagcgcgga agtcattcat
     6241 caactggcga cgctactcaa agccgggtta acgctttctg aagggctggc actgctggcg
     6301 gaacagcatc ccagtaagca atggcaagcg ttgctgcaat cgctggcgca cgatctcgaa
     6361 cagggcattg ctttttccaa tgccttatta ccctggtcag aggtatttcc gccactctat
     6421 caggcgatga tccgcacggg tgaactgacc ggtaagctgg atgaatgctg ctttgaactg
     6481 gcgcgtcagc aaaaagccca gcgtcagttg accgacaaag tgaaatcagc gttacgttat
     6541 cccatcatca ttttagcgat ggcaatcatg gtggttgtgg caatgctgca ttttgttctg
     6601 ccggagtttg ccgctatcta taagaccttc aacaccccac taccggcact aacgcagggg
     6661 atcatgacgc tggcagactt tagtggcgaa tggagctggc tgctggtgtt gttcggcttt
     6721 ctgctggcga tagccaataa gttgctgatg cgccgaccta cctggcttat cgcgcggcag
     6781 aaattgttgt tacgaatccc gattatgggt tcactgatga ggggacaaaa actcacgcag
     6841 atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttaca gggcgtagag
     6901 agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca aatccagcac
     6961 gatatctata gcgcggcgat ttttttgata ccgatttgca tttcatttga taccgctgaa
     7021 aaacgccgat acgctactta tttgatacta aatcggcgct tttcctttgc gttttagaat
     7081 gagtttttaa gcactcaccc aatctctttc aaaccggttt taacagtttg tggttttggg
     7141 taaggctgga ctttgaatag ctttgtgttg aggcgttttc tggccctctt gttcagaaac
     7201 cggatgtaac gaaactgtcg gaacttatga acactggc
//
LOCUS       DAZFVO020000213         7236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFVO020000213 DAZFVO020000000
VERSION     DAZFVO020000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.214"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..720
                     /gene="yneK"
                     /locus_tag="ACHKNB_RS22845"
                     /old_locus_tag="pgaptmp_004569"
     CDS             <1..720
                     /gene="yneK"
                     /locus_tag="ACHKNB_RS22845"
                     /old_locus_tag="pgaptmp_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HGU4197950.1"
                     /translation="KPVTMGRVFQTGVERVPFLFLNDFIEQFPMINPGAPIKRAHTPH
                     IEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLNTAI
                     LDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFANMLL
                     HYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRP
                     RSPVRPPIPATSKTPDLPERH"
     gene            870..2060
                     /gene="ydeA"
                     /locus_tag="ACHKNB_RS22850"
                     /old_locus_tag="pgaptmp_004570"
     CDS             870..2060
                     /gene="ydeA"
                     /locus_tag="ACHKNB_RS22850"
                     /old_locus_tag="pgaptmp_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HGU4197951.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2085..2750)
                     /gene="marC"
                     /locus_tag="ACHKNB_RS22855"
                     /old_locus_tag="pgaptmp_004571"
     CDS             complement(2085..2750)
                     /gene="marC"
                     /locus_tag="ACHKNB_RS22855"
                     /old_locus_tag="pgaptmp_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HGU4197952.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2962..3396
                     /gene="marR"
                     /locus_tag="ACHKNB_RS22860"
                     /old_locus_tag="pgaptmp_004572"
     CDS             2962..3396
                     /gene="marR"
                     /locus_tag="ACHKNB_RS22860"
                     /old_locus_tag="pgaptmp_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HGU4197953.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3416..3799
                     /gene="marA"
                     /locus_tag="ACHKNB_RS22865"
                     /old_locus_tag="pgaptmp_004573"
     CDS             3416..3799
                     /gene="marA"
                     /locus_tag="ACHKNB_RS22865"
                     /old_locus_tag="pgaptmp_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HGU4197954.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3831..4049
                     /gene="marB"
                     /locus_tag="ACHKNB_RS22870"
                     /old_locus_tag="pgaptmp_004574"
     CDS             3831..4049
                     /gene="marB"
                     /locus_tag="ACHKNB_RS22870"
                     /old_locus_tag="pgaptmp_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HGU4197955.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4106..5545)
                     /locus_tag="ACHKNB_RS22875"
                     /old_locus_tag="pgaptmp_004575"
     CDS             complement(4106..5545)
                     /locus_tag="ACHKNB_RS22875"
                     /old_locus_tag="pgaptmp_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGU4197956.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5570..>7236)
                     /locus_tag="ACHKNB_RS22880"
                     /old_locus_tag="pgaptmp_004576"
     CDS             complement(5570..>7236)
                     /locus_tag="ACHKNB_RS22880"
                     /old_locus_tag="pgaptmp_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HGU4197957.1"
                     /translation="KTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQNKQ
                     ELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNSSAA
                     AHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNHGSP
                     QTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFGDSA
                     NENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIEN
                     WALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGE
                     SILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT         1902 a         1763 c         1566 g         2005 t
ORIGIN      
        1 aagcctgtca ctatggggcg cgtatttcag accggcgtcg agcgtgtgcc atttctgttt
       61 ctgaatgatt ttattgaaca atttccgatg atcaaccctg gcgctcccat caaaagagcg
      121 catacaccac atattgaacc cctgcctccg gatcaccata ccgctgcaga ttacttacgc
      181 cagtttgatt tgcttgtcct gaattttatc tctcgcggta attttgtcat actcccccga
      241 ttatggaata actctgaggt tcacagatgg ttcgtcaata aagatcctaa cttgaacacc
      301 gccattctcg acataacgga cagcgaatta aaagaggatt tattgcaaag cctgatggat
      361 tcattaggtt ctaacaaaca tgtactaccc gaagtctgca tctgcttttt atccctttta
      421 gcagaacaag aatctcctca ttttcaggac ttgtttttat ttttcgccaa tatgttattg
      481 cactatcacc aatttatgaa tcccaatgaa agtgatttga atgacgtgtt gatgccagca
      541 tcattaagtg atgataaaat tatcaaacat atggcgcgca ggaccctcaa actttttgta
      601 aaaaatgaaa cgccgccaaa agttactcac gaagacctgg tgaaaaacag gcctcgctcc
      661 cctgtcagac cacctatacc cgcaacctcc aaaacgccag acctccctga acgtcattaa
      721 accgtgatgt taccgactct ctgacgcgtg aaagaatcag cgtcagagaa acggaaaacg
      781 cgatccagat cacaaatgca ttgtattcac atcattaacc gttttaagat catttcatca
      841 ctttttcgca actcacccga taatctgtta tgacaacaaa cactgtttcc cgcaaagtgg
      901 cgtggctacg ggtcgttacg ctggcagtcg ccgccttcat cttcaacacc accgaatttg
      961 tccctgttgg cctgctctct gacattgcgc aaagttttca catgcaaacc gctcaggtcg
     1021 gcatcatgtt gaccatttac gcatgggtag tagcgctaat gtcattgcct tttatgttaa
     1081 tgaccagtca ggttgaacgg cgcaaattac tgatctgcct gtttgtggtg tttattgcca
     1141 gccacgtact gtcgtttttg tcgtggagct ttaccgttct ggtgatcagt cgcattggtg
     1201 tggcttttgc acatgcgatt ttctggtcga ttacggcgtc tctggcgatc cgtatggctc
     1261 cggccgggaa gcgagcacag gcattgagtt taattgccac cggtacagca ctggcgatgg
     1321 tcttaggttt acctctcggg cgcattgtgg gccagtattt cggttggcga atgaccttct
     1381 tcgcgattgg tattggggcg cttatcaccc ttttgtgcct gattaagtta cttcccttac
     1441 tgcccagtga gcattccggt tcactgaaaa gcctcccgct attgttccgc cgcccggcat
     1501 tgatgagcat ttatttgtta actgtggtgg ttgtcaccgc ccattacacg gcatacagct
     1561 atatcgagcc ttttgtacaa aacattgcgg gattcagcgc caactttgcc acggcattac
     1621 tgttattact cggtggtgcg ggcattattg gcagcgtgat tttcggtaaa ctgggtaatc
     1681 agtatgcgtc tgcgttggtg agtacggcga ttgcgctgtt gctggtgtgc ctggcattgc
     1741 tgttacctgc ggcgaacagt gaaatacacc tcggggtgct gagtattttc tgggggatcg
     1801 cgatgatgat catcgggctt ggtatgcagg ttaaagtgct ggcgctggca ccagatgcta
     1861 ccgacgtcgc gatggcgcta ttctccggga tatttaatat tggaatcggg gcgggtgcgt
     1921 tggtaggtaa tcaggtgagt ttgcactggt caatgtcgat gattggttat gtgggcgcgg
     1981 tgcctgcttt tgccgcgtta atttggtcaa tcattatatt tcgccgctgg ccagtgacac
     2041 tcgaagaaca gacgcaatag ttgaaaggcc cattcgggcc ttttttaatg gtacgtttta
     2101 atgatttcca ggatgccgtt aataataaac tgcacaccca tacataccag caggaatccc
     2161 atcagacggg agatcgcttc aatgccaccc ttgcccacca gccgcataat tgcgccggag
     2221 ctgcgtaggc ttccccacaa aataaccgcc accaggaaaa agatcagcgg cggcgcaacc
     2281 atcagtaccc aatcagcgaa ggttgaactc tgacgcactg tggacgccga gctaataatc
     2341 atcgctatgg ttcccggacc agcggtactt ggcattgcta gcggcacaaa ggcaatattg
     2401 gcactgggtt catcttccag ctcttccgac ttgcttttcg cctccggtga atcaatcgct
     2461 ttctgttgcg gaaagagcat ccgaaaaccg ataaacgcga cgattaagcc gcctgcaatt
     2521 cgcagaccgg gaatcgaaat gccaaatgta tccatcacca gttgcccggc gtaatacgcc
     2581 accatcatga tggcaaatac gtacaccgag gccatcaacg actgacgatt acgttcggca
     2641 ctgttcatgt tgcctgccag gccaagaaat aacgcgacag ttgttaatgg gttagctaac
     2701 ggcagtaaca ccaccagccc caggccaatt gctttaaaca aatctaacat tggtggttgt
     2761 tatcctgtgt atctgggtta tcagcgaaaa gtataagggg taaacaagga taaagtgtca
     2821 ctctttagct aaccttgcat cgcattgacc aaaacttgaa ccgatttagc aaaacgtggc
     2881 atcggtcaat tcatttattt gacttatact tgcctgggca atattatccc ctgcaactaa
     2941 ttacttgcca gggcaactaa tgtgaaaagt accagcgatc tgttcaatga aattattcca
     3001 ttgggtcgct taatccatat ggttaatcag aagaaagatc gtctgcttaa cgagtatctg
     3061 tctccgctgg atattaccgc ggcacagttt aaggtgctct gctctatccg ctgcgcggcg
     3121 tgtattactc cggttgaact gaaaaaagtg ttgtctgtcg accttggagc actgacccgt
     3181 atgctggatc gcctggtctg taaaggctgg gttgaaaggt tgccgaaccc gaatgataag
     3241 cgcggcgtac tggtaaaact taccaccagc ggcgcggcaa tatgcgaaca atgccatcaa
     3301 ttagttggcc aggacctgca tcaagaatta acaaaaaacc tgacggcgga cgaagtggca
     3361 acacttgagc atttgcttaa gaaagtcctg ccgtaaacaa aaaagaggta tgacgatgtc
     3421 cagacgcaat actgacgcta ttaccattca tagcattttg gactggatcg aggacaacct
     3481 ggaatcgcca ctgtcactgg agaaagtgtc agagcgttca ggttactcta aatggcacct
     3541 gcaacggatg tttaaaaaag aaaccggtca ttcattaggc caatacatcc gcagccgtaa
     3601 gatgacggaa atcgcgcaaa agctgaagga aagtaacgag ccgatactct atctggcaga
     3661 acgatatggc ttcgagtcgc aacaaactct gacccgaacc ttcaaaaatt actttgatgt
     3721 tccgccgcat aaataccgga tgaccaatat gcagggcgaa tcgcgctttt tacatccatt
     3781 aaatcattac aacagctagt tgataacgtg acaacgtcac tgaggcaatc atgaaaccac
     3841 tttcatccgc aatagcagct gcgcttattc tcttttccgc gcagggcgtt gcggaacaaa
     3901 ccacgcagcc agttgttact tcttgtgcca atgtcgtggt tgttccccca tcgcaggaac
     3961 aaccaccgtt tgatttaaat cacatgggta ctggcagtga taagtcggat gcgctcggcg
     4021 tgccctatta taatcaacac gctatgtagt tattgtcagg ctctgatcac agagcctgac
     4081 atctccgtaa tatcttcctc aagtctcaaa tattttcgcc attgttggcg atgagatccc
     4141 gataccagta aaacgacgct ttacgtatcc gttccagcgt tcccttccct tcgttgtctt
     4201 tatcgacata aatcattccg tagcgctttt tcatttcccc tgttccggca gaaaccaggt
     4261 caatgcagcc ccacggggtg tagccaatta agtcaacacc atcttcaact acggcttttt
     4321 tcatttcccg aatatgggaa gcaaagtaat caatgcgata gtgatcgttc accgtgccgt
     4381 cagcttgtct ctggtcaacc gcaccaaatc cattttcgac aataaacagc ggcaactgga
     4441 aatgatccca gaaccagttg agtgaataac gtagccctgc cggatcaatt tgccatcccc
     4501 attcggatgt atcgatataa gggttgctga ccaaatctcg tggttcaaca taatccagtt
     4561 gcgggttatc gggcgaaaat tgtgtcgtaa aagacatgta gtagctaaag ccgataaagt
     4621 cgacacaacc actggcaaga atcgcgttat cttctggtgt gatatcgagg ttgaatcctt
     4681 tcctggcaaa gtaattcagc atatgttgcg gataatatcc acgagcatga acatcagtaa
     4741 accagtaacg acgatgcatt gctttcgtgg ccatcatcat atcgttgggt gcacacgtca
     4801 gaggatagat gggacacatg gcgatcatac agccaatgtt aaattcagga ttgatcgatt
     4861 ttccagtctg taccgccagg gcactggcaa ctaactcgta atgtaccgcc tggtacatta
     4921 tttgttcgcg ctcattgata tcttcctctg gcgaatacaa gataccggaa ttagtaaatg
     4981 gacacaggct ttcgctgaaa ttcacctgat tattgatttc gttaaacgtc atccagtact
     5041 ttactttttc tttatagcgc gtgaagaccg ttgatgcgaa gcggataaaa aagtcgatca
     5101 gtttacggtt tcgccagcca ccatattttg tcaccagatg ataaggcatc tcaaaatgtg
     5161 aaagcgtcac cacaggttcc attccctgct taaggcattc atcgaacaga tcatcataaa
     5221 attgtaaacc ctcttcattc ggctcctgtt cgtcaccttg cggaaagatt cgtgtccagg
     5281 caatggaagt tcgaaagcat ttgaatccca tctcggcaaa taactgaata tctgttttat
     5341 agcgatgata aaaatcaatt gcttcatgat tgggataatt aagcccgtcg ataacgcctt
     5401 ctgtcacttc acgcggcacc ccgtgagcgc cagcagtcat cacatcagcg atgctgatgc
     5461 cttttcctcc ttcattccag ccaccttcca actgatgcgc ggctaccgca ccgccccata
     5521 aaaaaccttt tttaaatcct gacatacaca actccttaac taaaatagat taccaccaga
     5581 cttccgcctg gatgccggcc ataaactcgt ctttgctgtt ctcattaaac ttaaaattcg
     5641 ataattcgtt atccagaata ttgatatacg ttccatagaa gcgaatttct ggtcgtgaac
     5701 ctaaaatgct ttcacccact ttcaatgcat gccagagtgt cgttttataa gccgattcat
     5761 ttagtgttac accctgctga gttttgttct gttgcttaaa ccagcctaat tcaagccccg
     5821 tctgattcca tgtattccag atccaggccg gtcttattac agtgcgaata ctgtcaaaat
     5881 cactatgagc accactttca taactataaa catcttcgcc gtgagaatag acaagcgcgt
     5941 tagccataat aatattgtca gtaagataca tctcgccctg agagattaaa cgccaggcga
     6001 tcccattggt atgatcacca tagtaatagc gaccatgcgc catcgtatta ctggcatctg
     6061 agaaactggc aaaactgctg gcataggaat tattggcaac ctgtaacgta aattcattaa
     6121 acgtatcgcg ttgcaggttt tgccgtaaaa cagaagtcag catccagcta tctttcattt
     6181 caaaataact gttgtcattt tcgttatctt gttgttgatc cgttttatta ggtgcggaat
     6241 atttagccat taatgataat gtcgcatcat cccataacgg gatattgcga taacgaacat
     6301 ccacagaatt ggtattcatc tggctggtac gcgaaaaatc acgagagtaa acatcgacat
     6361 catcacggct taaggacata tcaaacagcc ctacaccaag tgcccagttt tcaatcccca
     6421 cacccgcggc gacatccgtg gttaaggttt tccagtccag catctggatc tcatattgcg
     6481 ggagtttatg tttgccgacc cagaagtctg cctctggtgc gaagggtaaa aaacctcgcg
     6541 ttgtcaggta aatatcgctg aactgcatga tattttcatt ggcactgtca ccaaaccagg
     6601 catcgttata ctgctcaccc acgttgccat cataggtaac gaccgcattt gccgttttac
     6661 cgtcctgatt ataaacacgc tgatttaagg tcaggtcaaa ccaaccgctc atctcattac
     6721 caaaacgtcc cagagaaccc gctgcataag tttgtggtga gccgtgatta ccagtacccc
     6781 agcctgagcg gaagtacccc tgatagctga acccaatatc atcctttata tatttactga
     6841 tatctttcag ggtcacgctt tcgatagtgg ttttgtcgcc tttactttca ataacggcaa
     6901 cttgctgcga tccatttata gtatgcgctg cggcagacga gttctgttca ccgttttcat
     6961 ttttgatgtt atcagcgacg ggagatgtcg ctgatatttc agcaagcgag gctgatttat
     7021 atttgttttc cgtgatgctt ttttgtaagg tcgaaagtcg ggatttatat actcccagtt
     7081 cattctgtgt tgctttcagc tcttgtttat tttgcgacaa ttcattttca agcaattcaa
     7141 ggcgctgctc tatggttaat tttgttgcca tcgcgttcaa tggaaacaat gctgtaataa
     7201 tagaaaaaga caaaaggctg acgttaagcg tcttta
//
LOCUS       DAZFVO020000214         7066 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFVO020000214 DAZFVO020000000
VERSION     DAZFVO020000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.215"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            354..1826
                     /gene="cta"
                     /locus_tag="ACHKNB_RS22885"
                     /old_locus_tag="pgaptmp_004577"
     CDS             354..1826
                     /gene="cta"
                     /locus_tag="ACHKNB_RS22885"
                     /old_locus_tag="pgaptmp_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="HGU4197958.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVG
                     GGGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQR
                     LKDIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALR
                     EAERQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELA
                     KAESDVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSV
                     TNALNSAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEK
                     YSRIAQDLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEW
                     AKHLEKISRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVAL
                     MFSFIVGAPLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
     gene            complement(1823..2113)
                     /gene="cti"
                     /locus_tag="ACHKNB_RS22890"
                     /old_locus_tag="pgaptmp_004578"
     CDS             complement(1823..2113)
                     /gene="cti"
                     /locus_tag="ACHKNB_RS22890"
                     /old_locus_tag="pgaptmp_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="HGU4197959.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLY
                     PYSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
     gene            2138..2269
                     /gene="ctl"
                     /locus_tag="ACHKNB_RS22895"
                     /old_locus_tag="pgaptmp_004579"
     CDS             2138..2269
                     /gene="ctl"
                     /locus_tag="ACHKNB_RS22895"
                     /old_locus_tag="pgaptmp_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="HGU4197960.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(2484..2625)
                     /locus_tag="ACHKNB_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /pseudo
     CDS             complement(2484..2625)
                     /locus_tag="ACHKNB_RS22900"
                     /old_locus_tag="pgaptmp_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            2915..3151
                     /locus_tag="ACHKNB_RS22905"
                     /old_locus_tag="pgaptmp_004581"
     CDS             2915..3151
                     /locus_tag="ACHKNB_RS22905"
                     /old_locus_tag="pgaptmp_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197961.1"
                     /translation="MEKAPVPRPFPGSSFFAHMFFPGLSPDSVDNGVTAFEKGESARR
                     SRTTERSESVSEEAEEHRKLHFLLTHLCGISHSA"
     gene            complement(3340..3531)
                     /locus_tag="ACHKNB_RS22910"
                     /old_locus_tag="pgaptmp_004582"
     CDS             complement(3340..3531)
                     /locus_tag="ACHKNB_RS22910"
                     /old_locus_tag="pgaptmp_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HGU4197962.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            3599..3922
                     /locus_tag="ACHKNB_RS22915"
                     /old_locus_tag="pgaptmp_004583"
     CDS             3599..3922
                     /locus_tag="ACHKNB_RS22915"
                     /old_locus_tag="pgaptmp_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGU4197963.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            4131..4565
                     /locus_tag="ACHKNB_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /pseudo
     CDS             4131..4565
                     /locus_tag="ACHKNB_RS22920"
                     /old_locus_tag="pgaptmp_004584"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4600..5160
                     /locus_tag="ACHKNB_RS22925"
                     /old_locus_tag="pgaptmp_004585"
     CDS             4600..5160
                     /locus_tag="ACHKNB_RS22925"
                     /old_locus_tag="pgaptmp_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HGU4197964.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            5167..5400
                     /locus_tag="ACHKNB_RS22930"
                     /old_locus_tag="pgaptmp_004586"
     CDS             5167..5400
                     /locus_tag="ACHKNB_RS22930"
                     /old_locus_tag="pgaptmp_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HGU4197965.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
     gene            5552..5953
                     /locus_tag="ACHKNB_RS22935"
                     /old_locus_tag="pgaptmp_004587"
     CDS             5552..5953
                     /locus_tag="ACHKNB_RS22935"
                     /old_locus_tag="pgaptmp_004587"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HGU4197966.1"
                     /translation="MSEQEEREQKRREYRALLEGFWPDAFNFSRPRPLKVGILAELIA
                     DAEQRGLPFDSELLTSAIKFYTRRYVYQRALTTMKERTGLDGQIAGEVTPKQREYSLK
                     LIRRFAAKDKAEKRARQEEKAAKQSGINPGA"
     gene            complement(6094..6516)
                     /locus_tag="ACHKNB_RS22940"
                     /old_locus_tag="pgaptmp_004588"
     CDS             complement(6094..6516)
                     /locus_tag="ACHKNB_RS22940"
                     /old_locus_tag="pgaptmp_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGU4197967.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            6638..6916
                     /locus_tag="ACHKNB_RS22945"
                     /old_locus_tag="pgaptmp_004589"
     CDS             6638..6916
                     /locus_tag="ACHKNB_RS22945"
                     /old_locus_tag="pgaptmp_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197968.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
BASE COUNT         1845 a         1453 c         2022 g         1746 t
ORIGIN      
        1 tgtttctgga tgaggtcacg ggctttgtca tgctcatatc cggcagcaac aagggtgtcg
       61 agaagtcgtt tgtctgtttt gttcagttta tggcggggtg atttgctcac atcgttctcc
      121 gttttttcat gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg
      181 acgttctcaa tgcctgcctc cccatagtgt gatccttatt gcaaaaatga aaatccatgc
      241 tcttgacatg gacaatgctg agtagtaggt tttgctgtac ataaaaccag tggttatatg
      301 tacagtatgt tgtttttaat ttattgtttt aattatcaaa gaggaatttc tttatggata
      361 aagtcactga taattctcca gatgtggaga gcacagaatc tactgagggg tcattcccaa
      421 ctgttggggt tgatactggc gatacgatta cagcgacgct tgcaactgga actgaaaatg
      481 ttggtggagg cggtggagca tttggtgggg ccagtgaaag ttctgctgcg atacatgcaa
      541 ccgctaaatg gtctaccgcg cagttgaaaa aacatcaggc tgaacaggct gcccgtgctg
      601 ctgcggctga ggcagcattg gcaaaagcga aatctcagcg tgatgccctg actcaacgtc
      661 tcaaggatat tgttaatgac gctttacgtg ctaatgccgc ccgtagtcca tcagtaactg
      721 accttgctca tgccaataat atggcaatgc aggcagaggc tgagcgtttg cgccttgcga
      781 aggcagagca aaaagcccgt gaagaagctg aagcagcaga aaaagcgctc cgggaagcag
      841 aacgccaacg tgatgagatt gcccgccaac aggctgaaac cgcgcatttg ttagcaatgg
      901 cggaggcagc agaggctgag aaaaatcgac aggattctct tgatgaagag catcgggctg
      961 tggaagtggc agagaagaag ctggctgagg ctaaagctga actggcgaag gccgaaagcg
     1021 atgtacagag taagcaagcg attgtttcca gagttgcagg ggagcttgaa aacgctcaaa
     1081 aaagtgttga tgtgaaggtt accggatttc ctggatggcg tgatgttcag aaaaaactgg
     1141 agagacaatt gcaggataag aagaatgaat attcgtcagt gacgaatgct cttaattctg
     1201 ctgttagcat tagagatgct aaaaaaacag aagttcagaa tgctgagata aaattaaaag
     1261 aagctaagga tgctcttgag aagagtcagg taaaagactc tgttgatact atggttgggt
     1321 tttatcaata tataaccgaa caatatgggg aaaaatattc cagaatagct caggatttag
     1381 ctgaaaaggc gaagggtagt aaatttaata gtgttgatga agcacttgct gcatttgaaa
     1441 agtataaaaa tgtactggat aagaaattca gtaaggttga tagggatgat atttttaatg
     1501 ctttagagtc tattacttat gatgagtggg ccaagcatct agaaaagatc tctagagctc
     1561 ttaaggttac tggatatttg tctttcgggc atgatgtatg ggatggtacc ctaaagggat
     1621 taaaaacagg agactggaag cctttatttg tcactctgga gaagagcgcg gtagatttcg
     1681 gcgtggcaaa aattgtggca ttaatgttta gttttattgt tggtgcgcct cttggcttct
     1741 ggggaattgc aattatcaca ggtattgttt cttcttacat aggggatgat gagttgaaca
     1801 agcttaatga attgctaggt atttaatttc tctttagaga gtaatatatg aaacttggca
     1861 atcctactgg gattgccatt gctaggtaaa gaaaataaaa aaggttgtat gagtaccaag
     1921 gttctttttt catttttgtc gtgttttgta taaaagaaaa tatagttagt tttgaatatg
     1981 ggtatagtaa tacatgtata gatgcgatga atatgaaaag tgaaaatggg ggcgtcttat
     2041 agtttaataa caaatagaat agccatggga taagtgactc tagtaaattg tgtaggtagt
     2101 attttacggt cattatttat gttgttatag gggtagaatg aaaataacgt ttttgataat
     2161 tacaatatta tccttgtcgg gatgtcaagc aaattatatt agtgatgtac aaggagggac
     2221 ggtcgctcca tcttcctctt ctgaactgac agggatcgcg gttcagtaga aaagatcaaa
     2281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2341 ccgctaccaa cggtggtttg tttgccggat caagatctac caactctttt tccgaaggta
     2401 actggcttca gcagagcgca gatacccaaa acttgtcctt ctagtgtagt caaaatcagg
     2461 ctgatacttc aagaactctg ttactaactt ataaatctcg ctctgctaac ctggttacca
     2521 atggctgctg ccagtggcgt taagtcgtgt cctttccggg ttggacccaa gagaacagtt
     2581 accggagaag gcgcagcagt cgggctgaac ggggggttcc tgcatacagc ccggcccgga
     2641 gcgaactgcc taagtcgccc cgagatacca gaagacgtga gctatgagaa agcgccacgt
     2701 gtcccgcaag ggagaaaggc ggaaaaggta tccggtaatc gatatggcgg gaacgtgggg
     2761 gcgcaggagg gagcgattca ccggaaacgg tgatgatctt taagccacgt cgggtttcgc
     2821 caccgctggc ggatttcatg gttgagccgt aaggctccca cagatgcacc gaaaaagtgt
     2881 ctgttttgtg aagtccgtca ggggggcgga gcctatggaa aaagcgcctg tgccgcggcc
     2941 tttcccgggg tcttcgtttt ttgcgcacat gttctttccc gggttatccc cggattctgt
     3001 ggataacggg gttaccgcct ttgagaaagg ggagtccgct cgccgcagtc gaacgaccga
     3061 gcgtagcgag tcagtgagcg aggaagcgga agagcaccgg aagctgcatt ttctccttac
     3121 gcatctgtgc ggcatttcac actcggcatg atgcactttt catacaatcc gcactgattc
     3181 tgcatggtat aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     3241 ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     3301 tcacaggcaa gctgtgaccg tctccgggag ctgtatgtgt cagaggtttt caccgtcatc
     3361 cccgaagcgt gcgaggcagc tgcggtaaag ctcgtcggcg tggtcgtgaa gggcttcgca
     3421 gatgtccgcc tggtcgtcgg cgtccagttc attcagtttt tccagcagtg tcagcgtctg
     3481 gcttctgata aagcgggcca tgttaagggc tgttttttcc tgtttagtca cagttctgtt
     3541 tctccatgtg tggggagtct gtcggaggtt tttaatgata ccggtgaaac gcgaaaggat
     3601 gctcactata cgggtcacgg atgaggaaca tgcgcggctg ctggcacgtt gtgagggaaa
     3661 acaacttgcg tcatggatgc gtaaggtctg cctgggtgca ccgccatcga aaacgtcagg
     3721 tcttccgaca ctggcaccgc cgctgttgcg ccagttcgct tcggtcggca ataacctgaa
     3781 tcagatagcc cggaagataa acagcggaca gtggtcgggg catgaccggg ttcatgtggt
     3841 ggctgcactg atggccatcg gacgtgaact gagtgagctg agggatgagg tccgtaaaca
     3901 gggagaacgg gatgatagtt aagtttcatg ccaggggaaa aggcggtggc agtggtccgg
     3961 ttgattacct gctgggccgg gagcgtaacc gggaaggggc gagagttctg cggggtgctc
     4021 ccgaagaggt gcgggaactc atcgatgcga cgccgtttgc gaaaaaatac acctcggggg
     4081 ttctgtcttt tgcagagcag accctgccac cgggagagcg tgaaagggtg atggagagct
     4141 ttgagcgggt actgatgccc ggtcttgaaa aaaatcagta cagcatcctg tgggtggagc
     4201 accaggacaa ggggcgtctt gagctgaatt ttgtcatccc gaatatggag ctggcgagcg
     4261 gaaaacggct ccagccctat tacgaccggg cggaccgtcc gcgaatcaat gcctggcaga
     4321 cactggtgaa tcaccattac gggctgcacg acccgaacgc accggagaac aggcggacac
     4381 ttgtcacccc gaataatctg ccgaaagcga aacaggaggc cgcagaagcg attacgaggg
     4441 gactggaggc tctttaccat gccggagcgc taaaaacgcg tcaggatgtc actgaggcgc
     4501 ttacagcggc aggttttgag gttgtgagga caaccaggag cagtatcagt atcgccgacc
     4561 cggagggtgg gcgtaatctc aggctgaaag gggcgctgta tgagcaatct tttacaaatg
     4621 gggacagact tcgagaagaa actgaaagag cgtgccgtct ctacagagaa catgctgaac
     4681 agcgagttca ggaagctcga agcgtctgta gacagggaac tgaacttaaa cgggcagaaa
     4741 atcagagacg ctatcagcgc acacacagca gcggtgaaag aacaactgga gacgctgaac
     4801 agtgccgtga acgcgcagtt cagcgccaca gagaacgaac tggcccggcg gcaggaagag
     4861 ctgctctgga agctggtcaa agggcggata ctgtacccgt cgctgacggc gctttgcgtc
     4921 acgggcggga tttttctggc gtcatggggg ctgattcagt ggcaggaaag ccggattgcg
     4981 gcgaacatcc tggctatcag ggaccaggaa gagacgctgg cgaaactccg ggaaaaaaca
     5041 tggggtgtga cgtatcacga gggcaggaac gggaaatttc tggtgctgcc gtccggggtg
     5101 aagggggaaa acaactggac ggtggagaag aagaacgcag taagactggt cagggagtaa
     5161 gtgagtatga cagagctgga aatgcactta ctgaacgcct tagagcaatt gcagcaggac
     5221 tatacgcagc ggctgaaaga ctgggagagc gccttcacgg atttgcagca tatgttcgag
     5281 ctgacacgac aggagaacgg gcaactgagg gaacagtgcg acgacttgag tcagcaggtg
     5341 cagagattag ccgggcaggt ggaccgcttg agtcggttat ttatcacgaa cagcagataa
     5401 gggatgaaca ggaaaggcag gcacgcctgg aacgggagcg tgcgcggtcc tggcatgaag
     5461 agcaggaacg ggaagtacgg gtatggcgtg gtccgtcgct ggggcggtga atgtgtaaaa
     5521 tagcagtgcc tagctgagtg tggaggtgat tttgtccgaa caagaagaaa gagaacagaa
     5581 acgacgtgag tacagggcgt tgctggaggg attctggcct gatgcgttta atttcagccg
     5641 tccccgtccg ctgaaagtgg gtattctggc tgagctgatt gcagatgcag agcagagggg
     5701 cttaccgttt gattcggaac tcctgacatc ggcaataaaa ttttataccc gtcgttatgt
     5761 gtaccagcgt gctttaacta cgatgaaaga acgcacaggt cttgatggtc agattgccgg
     5821 ggaggtcacg ccaaaacagc gggagtattc cctgaaactg atcaggcgtt tcgctgcgaa
     5881 ggataaagcg gaaaaaagag cacgtcagga agaaaaagcc gcgaaacagt cgggaattaa
     5941 tccgggtgca taggtattca tcaggttaaa atttatcatg tgcgatcgcg gcagtttttc
     6001 gggtggtttg ttgccatttt tacctgtctg ttgccgtgat cgcgcctgga gcgtttcaac
     6061 ggtgcgtaca attaagggat tatggtaaat cccttactgt ctgccctcgt atccatcgag
     6121 ataaaccgca gaaatggtgt ctgccattag tttcattttt ttcggcgaag gcatatcgct
     6181 gtttgtgtat tttctgccag caaaggttgt tgtgctccgg agtgagtgaa aaaatttttc
     6241 tgagtggaat tcttttacac gttgctgtgc cgtttctgct gatactccgg cagctttgtc
     6301 tttcatcccc atatcccgga actgctgaaa gaacgggagc atcatacgta tggtgtctgc
     6361 tttctgggtg tagaagttag gatcaaatcc atcatctgaa gcataaacat aatgccgggt
     6421 atgtcgttcc aggctggtct ttggtgtgca tcctgtaaga gctattgttg ctgttatcag
     6481 aggtatcaga agcagcctgg cagcggcaga tctcataaac atctccttgc gtttttaatc
     6541 ttgatactca gggagttata tatccgaaga gtatctctta tcatacgtcg gtttactccc
     6601 aatgtgattt gatggtcaat gagtcaggaa taatcccgtg aaaaaatgtg tgccagtcct
     6661 gctttttagt gttctttctg cttcggttgc cagtcatgcg gacagcaatg tttttcaaca
     6721 aaaggccgag aagacaaaaa acggcattgt tgcggatgtt aatgccagac agaaaatgtt
     6781 cagtaacaga ctgaagatac aacttcagat cgatgagttg agagcagagc tggctggcac
     6841 cagagatgcc caaaaaagag aggatttgcg taaaaaactg gagtctctgg agcggcaaaa
     6901 gaaatcctta cgctgatggg ggcgtatttt ccccggtttc ttccataacc tttttaccgt
     6961 tcagatattt acgggcgagg cgaatgcgtc ttgcctggta taccgccgag taagcctggg
     7021 tgggattcac accattactg atttcactga ccacatgacg ctgatc
//
LOCUS       DAZFVO020000215         7032 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFVO020000215 DAZFVO020000000
VERSION     DAZFVO020000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 7032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.216"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(34..843)
                     /locus_tag="ACHKNB_RS22950"
                     /old_locus_tag="pgaptmp_004590"
     CDS             complement(34..843)
                     /locus_tag="ACHKNB_RS22950"
                     /old_locus_tag="pgaptmp_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4197969.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(863..1252)
                     /locus_tag="ACHKNB_RS22955"
                     /old_locus_tag="pgaptmp_004591"
     CDS             complement(863..1252)
                     /locus_tag="ACHKNB_RS22955"
                     /old_locus_tag="pgaptmp_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4197970.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            complement(1249..1575)
                     /locus_tag="ACHKNB_RS22960"
                     /old_locus_tag="pgaptmp_004592"
     CDS             complement(1249..1575)
                     /locus_tag="ACHKNB_RS22960"
                     /old_locus_tag="pgaptmp_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4197971.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(1572..2225)
                     /locus_tag="ACHKNB_RS22965"
                     /old_locus_tag="pgaptmp_004593"
     CDS             complement(1572..2225)
                     /locus_tag="ACHKNB_RS22965"
                     /old_locus_tag="pgaptmp_004593"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4197972.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(2225..2719)
                     /gene="yfdN"
                     /locus_tag="ACHKNB_RS22970"
                     /old_locus_tag="pgaptmp_004594"
     CDS             complement(2225..2719)
                     /gene="yfdN"
                     /locus_tag="ACHKNB_RS22970"
                     /old_locus_tag="pgaptmp_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4197973.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(2716..3657)
                     /locus_tag="ACHKNB_RS22975"
                     /old_locus_tag="pgaptmp_004595"
     CDS             complement(2716..3657)
                     /locus_tag="ACHKNB_RS22975"
                     /old_locus_tag="pgaptmp_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4197974.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDSSQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(3647..3826)
                     /locus_tag="ACHKNB_RS22980"
                     /old_locus_tag="pgaptmp_004596"
     CDS             complement(3647..3826)
                     /locus_tag="ACHKNB_RS22980"
                     /old_locus_tag="pgaptmp_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4197975.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(4002..4559)
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS22985"
                     /old_locus_tag="pgaptmp_004597"
     CDS             complement(4002..4559)
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS22985"
                     /old_locus_tag="pgaptmp_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4197976.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(4603..4803)
                     /gene="ymfT"
                     /locus_tag="ACHKNB_RS22990"
                     /old_locus_tag="pgaptmp_004598"
     CDS             complement(4603..4803)
                     /gene="ymfT"
                     /locus_tag="ACHKNB_RS22990"
                     /old_locus_tag="pgaptmp_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HGU4197977.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            4894..5568
                     /gene="ymfK"
                     /locus_tag="ACHKNB_RS22995"
                     /old_locus_tag="pgaptmp_004599"
     CDS             4894..5568
                     /gene="ymfK"
                     /locus_tag="ACHKNB_RS22995"
                     /old_locus_tag="pgaptmp_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HGU4197978.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            complement(5783..6298)
                     /locus_tag="ACHKNB_RS23000"
                     /old_locus_tag="pgaptmp_004600"
     CDS             complement(5783..6298)
                     /locus_tag="ACHKNB_RS23000"
                     /old_locus_tag="pgaptmp_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197979.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            6443..6754
                     /locus_tag="ACHKNB_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /pseudo
     CDS             6443..6754
                     /locus_tag="ACHKNB_RS23005"
                     /old_locus_tag="pgaptmp_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6769..>7032
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS23010"
                     /old_locus_tag="pgaptmp_004602"
     CDS             6769..>7032
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS23010"
                     /old_locus_tag="pgaptmp_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4197980.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYV"
BASE COUNT         1701 a         1885 c         1664 g         1782 t
ORIGIN      
        1 atttcagggg tcagtagtgc tctcacggtc acctcaatga acggtatcga gcagctttaa
       61 cagctcaggg aatcgggatt cgaagaaatg cggctgtgtc tcgcgcggat ttgcgggact
      121 ggtgatgttc ttgccgaaca tgcaaccttt cgctgtcagc gaccagaatt ttttgatgtt
      181 gttaatcgct gtacggctgt atcgttcgcg ctgctcgacg atccccagtt tcaccatctg
      241 gtgatatgcc tgattagccg tcaggcgtat accatactgt ttcagcagtg cactcagtga
      301 cagtgtcggg cgacttgagc catcgtgtgc atcagcagga gcatcaatgg catagcgcgg
      361 tgccagattc ggtaagccaa cagcctcctg gagtttctga caggcaccaa gcactgaaga
      421 gttagacagg tttaactccc ggcgcataaa gtccagcagg atcacgccag cctgcatctt
      481 gtcagcagcc tgtccggata atttttccgg tgcgctggtt accatatcga aagtacggat
      541 caccttcaga tggaatgacg ggctgatcca cattgcatag gcatacacca gttccttgca
      601 gacatacgtt ccccgttcat ttcccccatg aatcacactc accgggtcaa cacccaaatt
      661 ctgggtgttg gtcaattcat gaacaagctc aacagtttgt tggctggaaa gaaactttcc
      721 cggctccttg gttctggcat ttgcaccaga tgctactgct gcgcgatgca gatcgttcag
      781 gctgtaacgc ccataagcat cacgacgaac ttcaatacca tcaatgacca tcagattatt
      841 catacttcgt ttctcctctt gatcaggcgg ctgcacccgc cgttttctcg tacttactga
      901 tagtgatctc gaccttccct ttcgggataa ccggtcccca ctccaccagc attcttttca
      961 cctgactgtc gtcttcccac acacccgcat gggtcagggc gtcaaacagc gccttgttat
     1021 agttgtccag atcgcggatc cggttatccg gaggaaacaa cacgatctcc actgaagcag
     1081 gtgccgacgt tggtttcggc agacgacgta actgctcaac tattgctgcg cacgccgcgc
     1141 tctggaattt gcgccccgct gcgcttatca gactcttacc agcaaacgcc cctttgttgg
     1201 ggtgtcgcca gtacgtgttc acgctgggcg ggaaaggcaa gatcagcttc atactttcag
     1261 gcccctctca tgtaaccagt gggctgcacg cagcctggcg ttttcctcac cggcaagcag
     1321 tgcgcggata atcccgaccg cctcgctgtc gtcgtccttc accgcggtat gaagcgttat
     1381 cccccgggcc acgccacgct ttatcgtgat gacgcctttt ttctccagtg cgcgaagatg
     1441 ctccaccgct gcattcaccg aacggtatcc cagcatggtt gccacctcct gattggttgg
     1501 cggaaagcca cgttcttgct ggtaagaaat cagcatatcc agcacctgct gctggcattg
     1561 agttaacgtc gtcatgccgc catctccctg accagttttt ccgcctgctg gcgaacctgc
     1621 gccagaaagg cctcaccaca tgcctcaagt tcatcgcgcc cgatgtagct gattgccggt
     1681 cccttccagg tcttgtcgaa aacagcaata gcaccagcga agaaagcgcc tgtcggcacc
     1741 tgcttctcgt ccttcgggat aaaccaggct ggcagttcaa aaccaatacg cccgcgaata
     1801 aaagcaatat ggtctgcatc ttccggccac cacacttcgc tggtggcagc tttgatcagg
     1861 aaaacatagc gcccgccttt atcacgcatg gcactggcat gcttcatgat gtaacgcatg
     1921 ccggtgatgt attgcccctc atgctgactg gcgcggctgt acgggggatt accaaaggca
     1981 gcacctttaa gctccgcaag acgttctgac cagtcatgcg ccagcgcgtt gtcttccgca
     2041 gtgtaatacg cggcgcattt ggcgttatca ccatcagtaa acagatccag aacaaacggg
     2101 ccaaacaggg tgttaattcc ccagaaaatg ttgtccggcg tgcgccactg atcgcccact
     2161 tccttcagtt catgggctgg tttgttccgc agctccacca gcgcctggca atatttatta
     2221 ctcattaagc ccccacgtaa ttccctgaca gataccactc ttcacccgat gcagcgcgct
     2281 tgctgctttt ccgtaaacac cgctcacgac gcgcaagaaa attgtttcgc tcttgctggg
     2341 agtggctttc acggaatgcc gccatccaca ccgttgcagc acgacggtat aagcccctgg
     2401 actccagttc ttccgcctgg cgggtcaggc acaaaatcac ccggggatcg ttagtgccga
     2461 catagaaatt gcgcacaggt ctggattcac gaactggttg cggttccggc tcctgcggta
     2521 tctcagtcag ccgcgggaaa tgtctgcgtg tatccccttc acaacggtga gccacacgcc
     2581 cactctgacg taacttgctt gctgactgca gaacgcgctg tcgtgagtaa cctgcaaaag
     2641 catccgcaat gtctccggaa gtacaccccg gatgggcttc aatgaatttc tgaacgtcat
     2701 tcaaaagact catgatcacc ccctgaatcc tgccgggatc tggctgtagt ccacgttgtc
     2761 gtaactggct ttgaagtacg ggtcttcgcg tttttcggtg tacgtgctga cggacggcga
     2821 taagcgcagg gaaagctcat cccatttttc ccgcagcttc gacgggctga gcacgttacg
     2881 gcaccagaac ggatcgcgac tgacgcggct gtacatctcg cagatttgtt tatgagtacg
     2941 accatcctgc acacacatca ggcgaatttc gtttgcccag gctgtccagt tcggttcttt
     3001 gggacgaacc acctcgccgt cacattcggc ggcatgctcg tacagggcga tgattttttt
     3061 ccagagccac tgtgcgcagg tcaaatcatc ctgcgtcccc cactggcgct ttttagggct
     3121 gaatacaacc gcatcaggat ggcgagttaa aaaatcctgt tcagccgtct gcgtgtccgg
     3181 ttgcgaagag tccggacgag aaggtttttt atctgacgga tcatgttttg attttactga
     3241 cggatccccg ccagattctg acgggtgaaa acccgctttt ttgccagatt tcgacgcatc
     3301 aaattttgac gggtcagatt ttgatgcgtc agattttgac gggtcagaat ctgacagttg
     3361 agaaaatgcc gctgcctgaa gcttcgcaac gttaagctga taaacattcg acgcattgcg
     3421 gttaccctgg cgacgcgcct tacgcgttaa ccagccttct gcttccagcc gtgcgatagc
     3481 cgttctgacg gtactcatcc ccgcgccaat ctggcgggca atggtttcaa tcgatggcca
     3541 gcacacacct tcgtcattac tgaaatcagc caggcgggcc ataattgcca cgctggataa
     3601 tttcatgcct gacgctgcgc aaccatccca tacatagccg gttaatttag tgctcatgac
     3661 cgacctctat ttccctgaat ttacgacgaa actgttcgag cggactgaag cactcatgct
     3721 catagccttc gcggaggtag ataacccgtt gtgtttccgg ttcccaacga atgactctga
     3781 cgggcactcc gtagtgatct ttgaaccagc ggttaacttg tcgcaaagga ctgtctcctt
     3841 ctgccggttg aaatccccca cagcccactc tgcaaagctg tgggttacaa tttccctgtc
     3901 acctggtaca ttcactgcat agcaatattc caccttcgct tttccacccg gtacaggaag
     3961 cgcaatcagt tgcgagcgac ggtagtgtgt tgttaaactg ttcatgcgtt agtttctcca
     4021 caaccagaag caatcgacgc cacgacgccc ggagctgcac actcgcgggc gtcattactt
     4081 tctgaaatgc aaaagatttt gtagaccagt gctgcatgct cctgcagctt cgaaattgag
     4141 agatacagct cgtcgttaat tgctgtcttc tcatgcggtt ccactacacc gtcttcgatt
     4201 gctgaacgaa tctgttttga ataactgccg atctgttcaa tgacttccag cagacgctgg
     4261 ttaatatcgg cgttgtccac atcctcgacg tcaggaagcg acacaaagac gccatttgca
     4321 gactgcgcca cagcatcagc aatgaagtga gtgccaccag cacgctgtaa aaccattgcc
     4381 catcccagcg ggaaaatctg atcgccatct gcacgaaggc ggttgaataa agcgttttct
     4441 gttacatcga gccagtcagc cgcttcagcg taaccacccg gcaacgccgc gatagttttt
     4501 ctgacagctt tcacgtacca ctcaggctgt ttttctattt tccagtgatg cttacccacg
     4561 attagcctca tcgttctgtg gttaaaaatt gaaagtgttc tgctaatctt tcggatagat
     4621 atccggtctt aagtcagatt tcgtaattgc acctgacgtg cattgctcaa gttttttagc
     4681 cagcacaaaa ctggcttttt tatagccatt gaaaaccagc cgtaagtagc caggagttga
     4741 gccaactttt ccggccaact cgccctgctg ttctttggtt aaagagtccc aatacgcttt
     4801 catacaatat gtacctccgg tgtacatatt acatgattga aatgaacctt caagatactt
     4861 gtaccttaac ggtacaaggg ttttaatttc gttatgaaaa caatccatga catccggcgg
     4921 tctaacgcca gaaaactgag agatggtgtt ggagggaatt cttcctttgc cactatgatt
     4981 gatcgcgagc caacccagac cagcaggttt atgggagatg gtgctactaa aaatatcggt
     5041 gacagcatgg cacgacacat cgaaaaatgt ttcgacctgc ctgtcggatg gctcgatcaa
     5101 gaacaccaga caacgaacat cacaaaaaaa cctgatgttt caatcactaa taaacaaatc
     5161 acattagtcc ctgtcatatc atgggtacag gccggagcat ggaaagaagt tggatattct
     5221 gaggttgatt tgagcacagc agaaacgtat ccctgccctg taccctgtgg ggaaatgact
     5281 tatatcttgc gggtgatagg tgattcaatg attgatgagt accgcccggg agacatgatt
     5341 tttgtcgatc ctgaagtacc tgcctgccac ggtgacgacg ttattgcatt gatgcacgat
     5401 acaggtgaaa ccaccttcaa aaggttgata gaagatggga cacagcgtta tctcaaagcg
     5461 ttaaacccaa actggcctga gccttacatt aagatcaacg gtaattgctc tataattgga
     5521 actgtgattt tctcaggaaa accaagaaga tacaaaatca aagcctaatc aatgtttatg
     5581 aacctgcttc ggcaggtttt tttatacttg acaatgtacc tatgagatac ataatgtacc
     5641 caagagaaac aacaaacagg caggacgccc acgaagtagc cgcctggggc atatgaagtc
     5701 caggatgatt cgttagcaac aaaaaagcgc cctacaggac gcttagctct ttaacaatct
     5761 ggatatccac aacagtagta atctacagat tgccgttaag ttttctggac aactcctcaa
     5821 tggatggagg cgatacgtaa tccggatttt tattcatcag aaacttattt tcacagtgga
     5881 ggcacctgct tttatgaaaa agctcatctt cgctaaccgg gaatggttga agtatcgata
     5941 ctatcttttg tccaaaacat tttgggcaaa gatgcatggt tatgctgcca ccgttcacga
     6001 ttacctcctt cgagtataca aaagtacccg actcaagttg gttaaggata tagccttccg
     6061 tctgagcctc aaagttttcg aattctgcaa ttttagcttt gagagaagca tttatttctt
     6121 gataagagcc caccagttca acgagagaca cgcattcgcg ctgaatagac gcaagctttg
     6181 agttcagctc accaatagcc gcatttactt cagcttgagt ttttgcctcg ttcattagtt
     6241 ttgcaatctg ggctgtttca cgaatagccg tcattgctgc cgttaattca gcgatcacat
     6301 tgaatactct tattgttgtt ggggatatcc agattaaccg aatccttgtt gttggggaat
     6361 aaccaggtcc acctcgcctg atgtggctaa aagcaggcac ataacagcta agtattttca
     6421 accagagaga atccttagcg ttgtggtgaa tgcggctcag cgcacgcggg ttaaggttga
     6481 ggctgacagt cgaccttctg tggataccca cccgcctggt gtgcaacctt cgccaggcac
     6541 cgggaggcac ccggcaccac aagtttatgc tgtgtgtagt cctggcggta ccagtttgta
     6601 cccttgcttc cggctggtac cgtccttttt tgcaaaacag agaagagcat caccggacga
     6661 cgggctcata acccaatcca tccgggcggc tgccaccgca ggtgttcttc tctgttttgt
     6721 ggagaaacca accgaccttg cagggtcgat atgatgagga gcagcaaaat ggctagcgaa
     6781 cgcagtactg atgtgcaggc atttatcggg gagctggacg gcggcgtatt tgaaaccaaa
     6841 atcggcgcag ttctcagtga ggtcgcttcc ggtgtgatga acacgaaaac caaagggaag
     6901 gtctcactca atctggaaat cgaaccgttt gatgagaacc gtgtgaaaat caaacacaaa
     6961 ctctcatatg ttcgcccaac taaccgcggg aaaatttccg aagaagacac caccgaaacg
     7021 ccgatgtatg tc
//
LOCUS       DAZFVO020000216         6973 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFVO020000216 DAZFVO020000000
VERSION     DAZFVO020000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.217"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            444..1433
                     /gene="araF"
                     /locus_tag="ACHKNB_RS23015"
                     /old_locus_tag="pgaptmp_004603"
     CDS             444..1433
                     /gene="araF"
                     /locus_tag="ACHKNB_RS23015"
                     /old_locus_tag="pgaptmp_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HGU4197981.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1503..3017
                     /gene="araG"
                     /locus_tag="ACHKNB_RS23020"
                     /old_locus_tag="pgaptmp_004604"
     CDS             1503..3017
                     /gene="araG"
                     /locus_tag="ACHKNB_RS23020"
                     /old_locus_tag="pgaptmp_004604"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HGU4197982.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3032..4018
                     /gene="araH"
                     /locus_tag="ACHKNB_RS23025"
                     /old_locus_tag="pgaptmp_004605"
     CDS             3032..4018
                     /gene="araH"
                     /locus_tag="ACHKNB_RS23025"
                     /old_locus_tag="pgaptmp_004605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HGU4197983.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4185..4985
                     /gene="otsB"
                     /locus_tag="ACHKNB_RS23030"
                     /old_locus_tag="pgaptmp_004606"
     CDS             4185..4985
                     /gene="otsB"
                     /locus_tag="ACHKNB_RS23030"
                     /old_locus_tag="pgaptmp_004606"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HGU4197984.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4960..6384
                     /gene="otsA"
                     /locus_tag="ACHKNB_RS23035"
                     /old_locus_tag="pgaptmp_004607"
     CDS             4960..6384
                     /gene="otsA"
                     /locus_tag="ACHKNB_RS23035"
                     /old_locus_tag="pgaptmp_004607"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HGU4197985.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6391..6819)
                     /gene="uspC"
                     /locus_tag="ACHKNB_RS23040"
                     /old_locus_tag="pgaptmp_004608"
     CDS             complement(6391..6819)
                     /gene="uspC"
                     /locus_tag="ACHKNB_RS23040"
                     /old_locus_tag="pgaptmp_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HGU4197986.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1767 a         1633 c         1810 g         1763 t
ORIGIN      
        1 taattaatga gcacagtgat aattaatgac gaaatgaatg acgtgcattt tccacatctt
       61 tgagttgcgg ttattacacc atttcaaaaa ctcaacgcca ggtaatgcgg cctattgact
      121 ggttaaaaag aagacatccc gcatgggtac caaagacaac aaggatttcc aggctaatct
      181 tatggattaa tctgctgtgc attcgacaat ttgtctgaca aattggcttt cccttatgtc
      241 ttttcccgct aaatttatgc acgttctcac tgtaattctg cgatgtgata ttgctctcct
      301 atggagaatt aatttctcgc taaaactatg tcaacacggt cacttatctt ttagttaaaa
      361 ggtaatgctt tgttttccga ttaatttaac gaatgtcatt cgtttttgcc ctacacaaaa
      421 cgacactaaa gctggagaga accatgcaca aatttactaa agccctggca gccattggtc
      481 tggcagccgt tatgtcacaa tccgctatgg cggagaacct gaagctcggt tttctggtga
      541 agcaaccgga agagccgtgg ttccagaccg aatggaagtt tgccgataaa gccgggaagg
      601 atttagggtt tgaggttatt aagattgccg tgccggatgg cgaaaaaaca ttgaacgcga
      661 tcgacagcct ggctgccagt ggcgcaaaag gtttcgttat ttgtactccg gaccccaaac
      721 tcggctctgc catcgtcgcg aaagcgcgtg gctacgatat gaaagtcatt gccgtggatg
      781 accagtttgt taacgccaaa ggtaagccaa tggataccgt tccgctggtg atgatggcgg
      841 cgactaaaat tggcgaacgt cagggccagg aactgtataa agagatgcag aaacgtggct
      901 gggatgtcaa agaaagcgcg gtgatggcga ttaccgccaa cgaactggat accgcccgcc
      961 gccgtactac gggatctatg gatgcgctga aagcggccgg attcccggaa aaacaaattt
     1021 atcaggtacc taccaaatct aacgacatcc cgggggcatt tgacgctgcc aactcaatgc
     1081 tggttcaaca tccggaagtt aaacattggc tgatcgtcgg tatgaacgac agcaccgtgc
     1141 tgggcggcgt acgcgcgacg gaaggtcagg gctttaaagc ggccgatatc atcggcattg
     1201 gcattaacgg tgtggatgcg gtgagcgaac tgtctaaagc acaggcaacc ggcttctacg
     1261 gttccctgct gccaagcccg gacgtacatg gctataaatc cagcgaaatg ctttacaact
     1321 gggtagcaaa agacgttgaa ccgccaaaat ttaccgaagt taccgacgtg gtactgatca
     1381 cgcgtgacaa ctttaaagaa gaactggaga aaaaaggttt aggcggtaag taatttgccg
     1441 gaaaaattcc cctctgcatg atgcagaggg ggtgtgaacg accagtgatt cacggagacg
     1501 ttatgcaaca gtctaccccg tatctctcat ttcgcggcat cggtaaaacg tttcccggcg
     1561 ttaaggcgct gacggatatt agttttgact gctatgccgg tcaggttcat gcgttgatgg
     1621 gtgaaaatgg cgcaggaaaa tcaactctct taaaaatcct cagcggcaac tatgcgccaa
     1681 ccacgggttc tgtagtgatt aatgggcagg aaatgtcctt ttccgacacg accgcagcac
     1741 ttaatgcggg tgtggcgatt atttaccagg aactgcatct cgtgccggaa atgaccgtcg
     1801 cggaaaacat ctatctcggc cagctgccgc ataaaggcgg cattgtgaat cgctcattgc
     1861 tgaattatga ggcgggttta caacttaaac atcttggtat ggatattgac ccggacacgc
     1921 cgctgaaata tctctccatt ggtcagtggc agatggttga aatcgccaag gcgctggcgc
     1981 gtaacgctaa aattatcgcc tttgatgagc caaccagctc cctttctgcc cgcgaaatcg
     2041 acaatctttt ccgcgttatt cgtgaactgc gaaaagaggg gcgggtgatc ttatacgttt
     2101 ctcaccgtat ggaagaaata tttgccctca gcgatgccat cactgtcttt aaagatggac
     2161 gttatgtcaa aacctttacc gatatgcagc aggttgacca cgacgcgctg gtgcaggcga
     2221 tggtcgggcg cgacattggc gatatctacg gctggcaacc tcgtagttat ggcgaggagc
     2281 gcctgcgtct tgatgctgtg aaagcaccag gcgtgcgtac gccaataagt ctggcggttc
     2341 gcagtggtga aattgttggg ctgtttggtc tggtaggagc ggggcgtagc gaattaatga
     2401 aaggcttgtt tggcgggacg caaatcaccg ccggtcaggt ttatatcgac caacagccga
     2461 tcgatattcg taaaccgagc cacgccattg ccgcaggcat gatgctctgc ccggaagatc
     2521 gcaaagcgga aggcattatt cccgtgcact ccgttcgcga caatatcaac atcagtgcca
     2581 gacgtaaaca tgtgctcggc ggttgtgtaa tcaacaacgg ttgggaagaa aacaatgccg
     2641 atcaccacat tcgttcgctc aacatcaaaa cgccgggcgc tgagcaactg atcatgaatc
     2701 tctcaggcgg aaatcagcaa aaagccattc tgggccgctg gttatcggaa gagatgaagg
     2761 tcattttgct ggatgaacct acgcgcggca ttgatgttgg tgctaagcat gaaatttaca
     2821 acgtgattta tgcgctggcg gcgcagggtg tggcggtgct gtttgcctcc agcgacttac
     2881 ctgaagtcct cggcgttgcc gaccggattg tggtgatgcg ggaaggtgaa atcgccggtg
     2941 aattgttaca cgagcaggca gatgagcgtc aggcactgag ccttgcgatg cctaaagtca
     3001 gccaggcagt tgcctgagta aggagagtat gatgtcttct gtttctacat cggggtctgg
     3061 cgcacctaag tcgtcattca gcttcgggcg tatctgggat cagtacggca tgctggtggt
     3121 gtttgcggtg ctctttatcg cctgtgccat ttttgtccca aattttgcca ccttcattaa
     3181 tatgaaaggg ttgggcctgg caatttccat gtcggggatg gtggcgtgtg gcatgttgtt
     3241 ctgccttgct tccggtgact ttgacctttc tgtcgcctcc gtaattgcct gtgcgggtgt
     3301 caccacggcg gtggttatca acctgactga aagcctgtgg attggcgtgg cagcggggtt
     3361 gctgcttggc attctctgtg gcctggtcaa tggctttgtt atcgccaaac tgaaaataaa
     3421 tgctctgatc acaacactgg caacgatgca gattgttcga ggtctggcgt acatcatttc
     3481 agacggtaaa gcggtcggta tcgaagatga aagcttcttt gcccttggtt acgctaactg
     3541 gttcggtctg cctgcgccaa tctggctcac cgtcgcgtgt ctgattatct ttggtttgtt
     3601 gctgaataaa accacctttg gtcgtaacac cctggcgatt ggcgggaacg aagaggctgc
     3661 gcgtctggcg ggtgtaccgg ttgttcgcac caaaattatt atctttgttc tctctggcct
     3721 ggtatctgcg atagccggaa ttattctggc ttcacgtatg actagtgggc agccaatgac
     3781 gtcgattggt tatgagctta ttgttatctc cgcctgcgtt ttaggtggcg tttctctgaa
     3841 aggtggcatc ggaaaaatct catatgtggt ggcgggtatc ttaattttag gcaccgtgga
     3901 aaacgccatg aacctgctta atatttctcc tttcgcgcag tacgtggttc gcggcttaat
     3961 cctgctggca gcggtgatct tcgaccgtta caagcaaaaa gcgaaacgca ctgtctgatg
     4021 cttttttcca caacaattta acgttttttc ccaccacagc cagccgccac aacggttggc
     4081 tgttcttcat tgcaaatggc gacccccgtc acactgtcta tacttacatg tctgtaaagc
     4141 gcgttctgcg caaaacaata agaaaagaga aggaggagag ccgggtgaca gaaccgttaa
     4201 ccgaaacccc tgaactatcc gcgaaatatg cctggttttt tgatcttgat ggaacgctgg
     4261 cggaaatcaa accgcatccc gatcaggtcg tcgtgcctga caatattctg caaggactac
     4321 agctactggc aaccgcaagt gatggtgcat tggcattgat atcagggcgc tcaatggtgg
     4381 agcttgacgc actggcaaaa ccttatcgct tcccgttagc gggcgtgcat ggggcggagc
     4441 gccgtgacat caatggtaaa acacatatcg ttcatctgcc ggatgcgatt gcgcgtgata
     4501 ttagcgtgca actgcataca gtcatcgctc agtatcccgg cgcggagctg gaggcgaaag
     4561 ggatggcttt tgcgctgcat tatcgtcagg ctccgcagca tgaagacgca ttaatgacat
     4621 tagcgcaacg tattactcag atctggccac aaatggcgtt acagcaggga aagtgtgttg
     4681 tcgagatcaa accgagaggt accagtaaag gtgaggcaat tgcagctttt atgcaggaag
     4741 ctccctttat cgggcgaacg cccgtatttc tgggcgatga tttaaccgat gaatctggct
     4801 tcgcagtcgt taaccgactg ggcggaatgt cagtaaaaat tggcacaggt gcaactcagg
     4861 catcatggcg actggcgggt gtgccggatg tctggagctg gcttgaaatg ataaccaccg
     4921 cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt agtcgtagta
     4981 tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct tgccgttggc
     5041 atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg tgaaacaggg
     5101 aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc ctcttttaac
     5161 ctcagcgaac aggaccttga cgaatactac aaccaattct ccaatgccgt tctctggccc
     5221 gcttttcatt atcggctcga tctggtgcaa tttcagcgtc ctgcctggga cggctatcta
     5281 cgcgtaaatg cgttgctggc agataaatta ctgccgctgt tgcaagacga tgacattatc
     5341 tggatccacg attatcacct gttgccattt gcgcatgaat tacgcaaacg gggagtgaat
     5401 aatcgcattg gtttctttct gcatattcct ttcccgacac cggaaatctt caacgcgctg
     5461 ccgacatatg acaccttgct tgaacagctt tgtgattatg atttgctggg tttccagaca
     5521 gaaaacgatc gtctggcgtt cctggattgt ctttctaacc tgacccgcgt cacgacacgt
     5581 agcgcaaaaa gccatacagc ctggggcaaa gcatttcgaa cagaagtcta cccgatcggc
     5641 attgaaccga aagaaatagc caaacaggct gccgggccac tgccgccaaa actggcgcaa
     5701 cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg tcgaacggct ggattattcc
     5761 aaaggtttgc cagagcgttt tctcgcctat gaagcgttgc tggaaaaata tccgcagcat
     5821 catggtaaaa ttcgttatac ccagattgca ccaacgtcgc gtggtgatgt gcaagcctat
     5881 caggatattc gtcatcagct cgaaaatgaa gctggacgaa ttaatggtaa atacgggcaa
     5941 ttaggctgga cgccgcttta ttatttgaat cagcattttg accgtaaatt actgatgaaa
     6001 atattccgct actctgacgt gggcttagtg acgccactgc gtgacgggat gaacctggta
     6061 gcaaaagagt atgttgctgc tcaggaccca gccaacccgg gcgttcttgt tctttcgcaa
     6121 tttgcgggag cggcaaacga gttaacgtcg gcgttaattg ttaaccccta cgatcgtgac
     6181 gaagttgcag ctgcgctgga tcgtgcattg actatgtcgc tggcggaacg tatttcccgt
     6241 catgcagaaa tgctggacgt tatcgtgaaa aacgatatta accactggca ggagtgcttc
     6301 attagcgacc taaagcagat agttccgcga agcgcggaaa gccagcagcg cgataaagtt
     6361 gctacctttc caaagcttgc gtaggagcgg ttaatcaccc gtaagtggaa ctaacagcac
     6421 atcgacctca cttgaggcaa tcactctttt cgccgagcag gatgctcgcg aaaagaaact
     6481 gtgattgtga ttaccgcaaa tcaccaaatc gaaatgatgt ttgtgacata cttccagaat
     6541 atgttcgctt aactcaccgt aggcaataaa cgtcttgtca acgggatacc ccgcatcctg
     6601 aattaactta tcaagaaagc tttgcgtttc ttcttgcatc acgctgcgta aatcttcaag
     6661 catcggcgcg gctaattgat tgtacatttc cgggtcggaa gcgagagtaa ttaaactgat
     6721 gtgtcccttt actggcctgg cgatagatac cgcttttgcc agcagttgct gactttccgg
     6781 tgtaaccgca acagcgacaa gaatattgct atagctcata acctgttcct tattctgtga
     6841 acttcaggtg acattaaagc tttactccta atggagaaac gacgcaatcc caactcggtc
     6901 aaacaggtaa atattagtct gattattagt aaagacaatt aatcaatcac tcccggtgat
     6961 catataattt caa
//
LOCUS       DAZFVO020000217         6920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFVO020000217 DAZFVO020000000
VERSION     DAZFVO020000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.219"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(78..1727)
                     /gene="pgi"
                     /locus_tag="ACHKNB_RS23045"
                     /old_locus_tag="pgaptmp_004609"
     CDS             complement(78..1727)
                     /gene="pgi"
                     /locus_tag="ACHKNB_RS23045"
                     /old_locus_tag="pgaptmp_004609"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGU4197987.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      1964..2160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2252..3601
                     /gene="lysC"
                     /locus_tag="ACHKNB_RS23050"
                     /old_locus_tag="pgaptmp_004610"
     CDS             2252..3601
                     /gene="lysC"
                     /locus_tag="ACHKNB_RS23050"
                     /old_locus_tag="pgaptmp_004610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HGU4197988.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3746..4672
                     /locus_tag="ACHKNB_RS23055"
                     /old_locus_tag="pgaptmp_004611"
     CDS             3746..4672
                     /locus_tag="ACHKNB_RS23055"
                     /old_locus_tag="pgaptmp_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HGU4197989.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4708..5499)
                     /locus_tag="ACHKNB_RS23060"
                     /old_locus_tag="pgaptmp_004612"
     CDS             complement(4708..5499)
                     /locus_tag="ACHKNB_RS23060"
                     /old_locus_tag="pgaptmp_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HGU4197990.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5505..6632)
                     /locus_tag="ACHKNB_RS23065"
                     /old_locus_tag="pgaptmp_004613"
     CDS             complement(5505..6632)
                     /locus_tag="ACHKNB_RS23065"
                     /old_locus_tag="pgaptmp_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HGU4197991.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAELVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1706 a         1797 c         1658 g         1759 t
ORIGIN      
        1 gttaaatcac agacaagggg atttatctga taaaacaatg ccgacataac gtcggcattg
       61 ttattaaggt ttttaaatta atcgcgccac gctttatagc ggttaatcag accattggtc
      121 gagctatcgt ggctgctgat ttctttatca tctttcagct ctggcagaat acggtttgcc
      181 agttgtttac ccagctccac gccccactgg tcgaaggtga agatgttcag gatcacgccc
      241 tgagtaaaga ttttgtgctc atacagcgca atcaacgccc ccaggctgaa cggagtgatt
      301 tcacgcagca ggatggagtt ggtcgggcgg ttaccttcga acactttgaa cggcaccacg
      361 tagtcaagcg ttgccggatc tttaccctga tcgcgatatt cctgctcaac cacttcgcgg
      421 gatttaccaa acgccagcgc ttcagtctga gcaaagaagt tagacagcag tttctggtgg
      481 tgatcagaga gcgggttatg ggtgatagcc ggagcgatga aatcgcacgg taccattttg
      541 gttccctggt ggatcagctg gtagaacgcg tgctgaccgt tagtgcctgg ttcaccccag
      601 ataatcgggc cagtctggta atccacaacg ttaccgttac ggtcaacata cttaccgttg
      661 gactccatat tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc
      721 agaatcgcct cagtttccgc accaaagaaa ttgttgtacc agatgccaat cagcgccagc
      781 agtacaggca ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg
      841 ccagaaagca gttcaacgaa gttatcaaag ccgatggaga gaacaatcga caggccaatc
      901 gctgaccaca aagagtaacg gccgccgacc cagtcccaga actcgaacat gttggcagta
      961 tcaataccaa actcgccaac ggctttggca ttggttgaga gcgccgcaaa gtgtttcgca
     1021 acgtgttttt catcacctgc cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc
     1081 atggtttcct gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt
     1141 acttttttca gcacttccgc gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc
     1201 aggtggtttt tgtacggacg cagtgcttcg gtcaccatgt acgggccgag gtcagaaccg
     1261 ccgatcccga tgttcactac gtcagtgatt gctttgccgg tataaccctt ccactcaccg
     1321 gagataatcg cttccgagaa ggttttcatc ttctccagca ccgcgttgac ttccggcatt
     1381 acgtctttgc catcaaccaa aatcggggta ttgctacggt tacgcagcgc tacgtgcagc
     1441 acagcgcggt tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc
     1501 gccagatcgc actctttcgc cagatcttgt aatttcgcca gcgtctcttc agtgatgcgg
     1561 tttttggagt aatccaccag catctgatcg ccgaaggttg cggagaactt agaaaaacga
     1621 tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc
     1681 tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt
     1741 ctgattttga gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg
     1801 ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag cgcagtgaag catttattag
     1861 ctgaactact gaccgccagg agtggatgaa aaatccgcat gaccccgtcg ttgacaaccg
     1921 ccccgctcac cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc
     1981 gttgcccaag taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc
     2041 gccgaggtga ttgaacggct ggccacgttc atcatcggct acaggggctg aatcccctgg
     2101 gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga
     2161 aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg
     2221 ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg
     2281 cggtaccagc gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga
     2341 tgccaacgtg cgtttagtag tcctctcggc ttctgctggt atcactaatc tgctggtcgc
     2401 tttagcggaa gggctggaac ctggcgagcg attcgaaaaa ctcgacgcta ttcgcaacat
     2461 ccagtttgcc attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg
     2521 tctgctggag aacattactg ttctggcaga agcggcggcg ctggcaacgt ctccggcgct
     2581 gacagatgag ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgaaatcct
     2641 gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga
     2701 ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct
     2761 gctcccacgc ctcaatgacg gcttagtgat cacccaggga tttatcggta gcgaaaataa
     2821 aggtcgtaca acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga
     2881 ggctttacac gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga
     2941 tccacgcgta gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga
     3001 gatggcaact tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag
     3061 cgatatcccg gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg
     3121 caataaaact gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct
     3181 gctcactttg cacagcctga atatgctgca ttctcgcggc ttcctcgcgg aagttttcgg
     3241 catcctcgcg cggcataata tttcggtaga cttaatcacc acgtcagaag tgagcgtggc
     3301 attaaccctt gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatctct
     3361 gctgatggag ctttccgcac tgtgccgggt ggaagtagaa gaaggtctgg cgctggtcgc
     3421 gttgattggc aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact
     3481 ggaaccgttc aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct
     3541 ggtgcccggc gaagatgccg agcaggtggt gcagaagctg cattttaatt tgtttgagta
     3601 atatttccct actggcgtat aacgctcacc gattgttata cgccatcctc actgaaaaca
     3661 tagattaaat aatatctgaa aagcaaaatc actcctgtag aacagtatta taatcaccga
     3721 aaaatatcat ttaacaggag tgttaatggc tactttaatg gaaaaagatg cacttttaaa
     3781 cggagccagt cagtgcatcg cgtttttatc aaatatcatt gataattgct cagtttcgtc
     3841 gcatcaggat tcaggcgatg ctctcaagcg attagtcagc tatcgcgatt atctttattc
     3901 tacccccgct gaacttgttg attttacaca aggtaagatt ctccttcagc aggtaagaac
     3961 tcaatatcag catgaattca acaatactac gcattcagaa aataaagcat cttttgattc
     4021 tatttggcag cgtctgacaa atcatgaagt cacaccgcaa cagcatccca ttggttttgt
     4081 tttaggtggg caacccggag caggtaaatc atctctgatt gaattagcca aaagagagac
     4141 aaaagataat ataatgatta ttaacggtga tgattttcga tttctacacc ctgattttaa
     4201 ttatatctac caaaattatg gggatgattt tgttacccat acagcgaaat tttccggcga
     4261 aacggtcgaa cgtgcaattg agagagcgat agtcagtaaa ctcaacattg ttgtcgaagg
     4321 aacatttcgt aatgccgcga cgccgttaca aacattaaaa aaactcaaag atgcaggcta
     4381 ccaaacagaa gtcatgataa aaacaacctc agcggcatta agctgggaaa gcaccaatga
     4441 acggtataat aaggacaaag aggcgggaaa tattgcccga aaagtggata aaaaccacca
     4501 tgatatcgtc accggtttgc tggcagaaaa tgcccgcaag gtgtttgctt cgaacctgtc
     4561 agataagttt gcggtgtatt cgcgtgagaa gatgatattt agttcacaag cggcaacgaa
     4621 tgacgatatt gcaacactta tacagaatga aatcagcggg aatacacaat aataataaga
     4681 gctgatggcg tgaaataacg ccgggcttta tgcccggcgc attaccgtct ggtacattaa
     4741 gctatccagt tccttaacgt ccccttctct gctatccggt aacagcggtg gaatttcccc
     4801 acgcgccagt tccgcacgaa taaacgcatg taacaatgga cggcgcggac catattccgc
     4861 ttctcctgcg cgctgtttac gttccagtaa ttcgtctatt tctgcacgta acggcgcatc
     4921 aagttcgctt cccgccagta actcagcaaa gcgcattggc ggtacgcctt tacccgcttc
     4981 gacccagcgc acggccagca gcggacgcag cacgtaaaag tattttttca gtcgaacttc
     5041 gtcgccctgc aaatatccgg ggaagttttt ctgcgccatc gaatagtaat gccagcgcgc
     5101 acgcagcgga gaaaaccaca ttgggaccat tgccttaaac gccgttatcg tttcttcatc
     5161 ttgttgataa atcaccggag aatccagcca ttctatcagc gtcgggttcg cgcctttcaa
     5221 taatcccagc gccttacgcc actcccaacc ggaaacatct aactcgtcgt cgatgggcag
     5281 ctcaataaca tcgcgcggtg actctactcg cagataccat tccagcgggt gaacatacaa
     5341 aaaccgtaca tcgtagtcgc tgtcgggcga ggcaaatccc cagccgcggc tccctgattc
     5401 acaggcgtac agtactttca catcataccg ccgttcaatt tcttttaact gctgcgatac
     5461 cctttcgcgc atcgcagcac tgactccatt taatggcatg ttactcaccc tttgatatta
     5521 accaacccat gcagaacctg atccagaccg ccaaatacag agatgttatc gatccgctca
     5581 gccactctct ccagcgtttc tagctctttt aaacgcaacg ctaccggatt gttctccatg
     5641 actttggcgg tgttcagtaa cgagcgcgtc gctgccgttt cttcacgtcg gcgaatcaca
     5701 ttcgcctgcg cagatttctc tgcctcaacc agctgcgcga ggatattttt catatcacca
     5761 ggcaggacga tatctttcac ccctaatgag gcaatctcca taccaaatgg caacatgcgc
     5821 gatttcacct gttcgctgac aacgtcatca atgacctgtt tatcttccag cagttcatcc
     5881 agcgtacgag tacctaccgc ttcacgcagg gcaaattgca attcccgata caaatgatca
     5941 atcggcttcg ttaactgact aaatgccagc aacacgtcgc tgtagcgcca gttagcggcg
     6001 aggttaatcc gcagattgac cttatctttc gttaagattt cttgcccgct gacttccagt
     6061 acctgcaagc gggtgtcaac caattcagcg tcaaccagat ggttaatttt ccagtatgcg
     6121 gtcagccccg gcggtaacag cgcctgcgtt tcaccatcga ttttcagcac gcctacatgc
     6181 caggccggaa cctgcaccgt tagtattgca tcgcggcctt taactgcccc tttgcgccgt
     6241 ggttgcaata ccgcattcat cacctccgtt tgtacctgga cttgctgcgt attcatccgc
     6301 accagtgtca aatcatcctc tacacgccag tacaaacggc gcgttgatgg cggcaggatc
     6361 tcctgcaaaa taccgtccat atacaacgcc ccggcttcaa tctccgacaa gtctgccacc
     6421 aggcaatatt tctccaccca atcaggctga aagcggcgca aataatccgc cagcacgtcg
     6481 ggaacttcgc tgccatcgag cgtaatcagc aagacttctg gcgtatttaa ccagggtaaa
     6541 agatgctcgc ctgcatgtag cactttgtag taatcgccgt ttctggagag taatgccagc
     6601 tgatcttttc ttacgcttat ttttttaatc attatctatt ccttgcttaa cagccgtcgc
     6661 ggaagcagac acgaagcgac aggggaattc ccttttgcct gttacccggc atctttatcg
     6721 gcgacgttat cggggatcgc tgactgcgca atccctcact ttttatttaa taagttaaaa
     6781 ccagtattca tttcaggaat gaatgtggga atcgaaccca taattggcgt caaaggctga
     6841 cggaacacgc ccggaaaaca gcctggccgt tcttggtgtg ccgacggggt ttgaccccct
     6901 ttgtaattca gtttcaacgc
//
LOCUS       DAZFVO020000218         6884 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFVO020000218 DAZFVO020000000
VERSION     DAZFVO020000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.220"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..327)
                     /locus_tag="ACHKNB_RS23070"
                     /old_locus_tag="pgaptmp_004614"
     CDS             complement(<1..327)
                     /locus_tag="ACHKNB_RS23070"
                     /old_locus_tag="pgaptmp_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4197992.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCD"
     gene            complement(413..1072)
                     /locus_tag="ACHKNB_RS23075"
                     /old_locus_tag="pgaptmp_004615"
     CDS             complement(413..1072)
                     /locus_tag="ACHKNB_RS23075"
                     /old_locus_tag="pgaptmp_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGU4197993.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            1412..1705
                     /locus_tag="ACHKNB_RS23080"
                     /old_locus_tag="pgaptmp_004616"
     CDS             1412..1705
                     /locus_tag="ACHKNB_RS23080"
                     /old_locus_tag="pgaptmp_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197994.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYEEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            1949..2317
                     /locus_tag="ACHKNB_RS23085"
                     /old_locus_tag="pgaptmp_004617"
     CDS             1949..2317
                     /locus_tag="ACHKNB_RS23085"
                     /old_locus_tag="pgaptmp_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4197995.1"
                     /translation="MSAELKLVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIADGVDVIRATRFK"
     gene            2351..4009
                     /locus_tag="ACHKNB_RS23090"
                     /old_locus_tag="pgaptmp_004618"
     CDS             2351..4009
                     /locus_tag="ACHKNB_RS23090"
                     /old_locus_tag="pgaptmp_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGU4197996.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQISS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            4011..4979
                     /locus_tag="ACHKNB_RS23095"
                     /old_locus_tag="pgaptmp_004619"
     CDS             4011..4979
                     /locus_tag="ACHKNB_RS23095"
                     /old_locus_tag="pgaptmp_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HGU4197997.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRIQRRFSALTSLQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            4979..5842
                     /locus_tag="ACHKNB_RS23100"
                     /old_locus_tag="pgaptmp_004620"
     CDS             4979..5842
                     /locus_tag="ACHKNB_RS23100"
                     /old_locus_tag="pgaptmp_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4197998.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKSSSVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYRDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            5921..6346
                     /locus_tag="ACHKNB_RS23105"
                     /old_locus_tag="pgaptmp_004621"
     CDS             5921..6346
                     /locus_tag="ACHKNB_RS23105"
                     /old_locus_tag="pgaptmp_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HGU4197999.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSRAQVSQFSGHTGN"
     gene            6546..6702
                     /locus_tag="ACHKNB_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /pseudo
     CDS             6546..6702
                     /locus_tag="ACHKNB_RS23110"
                     /old_locus_tag="pgaptmp_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
BASE COUNT         1822 a         1498 c         1847 g         1717 t
ORIGIN      
        1 atcgcagtca agtatatttg ccagaggaat cacctggctt gccagaggtt cagccttgcc
       61 gttctcccac tggatgatgg tttcttcatc aactccaagc agcgtggcga gttcagcggt
      121 ggatagaccg cagctttcgc gttgggtgcg gatgccatca cggcaatctt ttttgtttct
      181 cggaggtaac agaatatgtt ttagttgttg gattttttcg attccaacct caaggagtac
      241 gtgacggttt ctttccgata cagactcgag actttcatga agcagcgatt ctacttgttc
      301 gataagcatc aattgtgaag tagacataaa aaaacctcgt aaccaatatt tcgaaatcta
      361 ggttacgagt gagtaatgta ggtgtcaaga gcaattaatg ttttttgttg aattaaattt
      421 gcatacgcat ctcaacgaca acaccaataa tttcgcaatc cccattgatg gggatcagtg
      481 gatagcgtgg gtttagtggc tttaggaaac attgcccagc gtcttctatg tactgtttaa
      541 gggttgcctc tccggagttt ttgagcctgg cgacgacata ttttcctgaa aaaacttctt
      601 tgtcagggtt aacaagaatg ttcatgcctt caaaaataga gaactctcca ttctgtgatg
      661 tcattgagtc ccctttcact tccagccaga agccgtgatc gcccgcatat atatcactac
      721 caatccattc ttctggataa gctatgtaat cttcgcagtt ccaggggcca gcactcaccc
      781 atgttagttt cggatatcta taacggtttg gagaaactgg cttaggattt cggacgtttt
      841 gaatgctatc tgtaggtgat ggggcgctgc cgtcaagaag ccatttagga gagcatttca
      901 atgcatccgc gattctaaat aggtttgaag atcttgtgtc ttgtgagtct cctaactcta
      961 ttttgctgat tgttacacgc gagacaccag tcaattttgc caggtcatct tgtgacattc
     1021 ctagctgtac tctgcgcatt aacagtctgc caccaaagct atcgtgatcc atcttctccc
     1081 ccttcttgtc gaaataatcg tttcgaaaca aatatagcaa aaaatcttga aatctaggtt
     1141 tcggaaaatt aagattaatc gaaacaaacg tgtcgaggtg gatatggatt tgtatgaaat
     1201 tctaaaaaac atgtttggat cgaatgttga aataggtcgt tattttcccc gaaggggaag
     1261 ggctagaacc ggacaggctg taggtaagtg gaaaacccga ggtgtgccgg aagatgtggc
     1321 gatcctttgt catctggatc ccaaaattcc ttatcagcat ccgtcgctca tgaatgccag
     1381 tcatgaatcg tgatgttctg ccggaggctg tatgtcccct gattacgttc agatggagat
     1441 gcctgccagg tacagccagg cagattcaga gtggattaag cggcagttac agagcctgcc
     1501 ttcctcactg agacagaagg tcgccctgaa atacgaggag gtttacgaag ttacttttga
     1561 ttccgagcct gtttcattcc gtaaggagaa cagagcgagg catgaagcca atgtacggct
     1621 gcgcaggttc gttgaaacac acgggcgtgc attacagggg tatacgactc agccgcccct
     1681 ggcaggaacg caacagcgta cctgaatggt gttggtctta aaggtgaccg acgggctgag
     1741 accccacttc tcaggatgca cctgtgtact accaagctag tgcataagtt gagagggggg
     1801 ggtaaggggg ggagtgcccg tgtgttagtg cgaagcactg gaacgggatc ttccaacaga
     1861 cgggtacaga ggttaggtag atctcgatct aaaggggcat ccctgaaaaa acggatgtat
     1921 cagcaaacta gtacatcatg gttaaaatat gagcgctgaa ctgaaactgg tactaatcaa
     1981 ccttctggaa gaggaattcg gacatggaat ggacaaggtc acattcgact acgttctgca
     2041 aaagaaaatc aaatctctgg gctgtgagtt gcaacgctgt ttcactgtca ggctgaaagg
     2101 tgatcgcagg ggatttatcg atcttctggt tatttctccc gatggtcagc gttgcgcagt
     2161 tgaggtggat aaccgatcgc cccgtcagcg ttcactgatg aaaattcgag cgcttcctga
     2221 aggcatttcg ggatttgttt ttctccgtga tggcaaacat ccacagcgtt atatcgccga
     2281 tggtgtggat gtcatccggg cgacccggtt taagtaatca ctgtatcagc cctgtcatcg
     2341 ggagaatacc atgctgaata tcaagccgaa ttttgctcag gaacgtgcgc tgaatatgtt
     2401 gcgtcatgac tggaaaacat atcagtcctt catgatgtac atgcctacag ggagtggtaa
     2461 aaccggcctg gcggcgttca tcactgctgg tctggtaagc cgtggcatgc gcgttctgtt
     2521 tgtcgttcct tataccattt tgatcaacca gacagcacag cgctttgttc agtacgggct
     2581 ttcaggggat gaaatcagct ttatctggcg tgatcatccc aataacgacc cgtcacgact
     2641 gattcagatt tccagtgcgg atacgctgat tcgccgtgat tttcctcaga atatcgacct
     2701 gctgattatt gatgaagcgc atctccgtaa gcgccgcatc ctcacggaaa tcgaacgact
     2761 ggttgcagag ggaaatgtga aggtgattgg gttgtccggt acaccctttt catcgttcct
     2821 ggggcgttat taccagcgtc ttctcaagcc gaccactata ggggaactga tccagcgtgg
     2881 cgaactgagc aattacgaat tttatgcgcc cacgaaaccg gatctggaag gtgtaaaaac
     2941 gaaagtctca atggagtacg gcagggatta cgacgaatca caactggcag agattatgtg
     3001 cggcgcggat ctggttggcg atattgtcga taactggctg tgtaacggaa gggatttacc
     3061 gacagtggcg ttctgcgtca ataaaacgca tgccagcttc gtgaccatgc agttcaacaa
     3121 agccggaatt aacgcagagg tgatggttgc tgaaacgcca catgatgagc gtcagttaat
     3181 gatcagtcgt tttgaaacgg gagcgacgaa aatcattgtc agcgttggcg ttctggtggc
     3241 gggatttgac agtgatgtcc ggtgcatcat ttacgcacgc ccgacaaaat cagagattcg
     3301 ctggcttcag gcgattggga gaggattgcg tacagcgcca ggcaaggagt catgcctgat
     3361 tttcgaccat tccggtacgg ttcatcgcct gggatttcct gaaaatattg agtatgacga
     3421 actgcccggc aaaaacgacg ggatgcaggt gtcatcatca cgtggtaacg aaattcggga
     3481 agaaaaactg ccgaaagaat gtccggggtg tcatttcatg aaaccggcag gtgtttatgt
     3541 ctgtccgaaa tgcggattta aaccgctggc tggtgaggat gtgaacacag atacccgccg
     3601 caatatcagg aaactcagca aggacgaaaa aatttacacc aaaagcgaca aacaggcctg
     3661 gtggagtcag atcaaattct atcagcgtca gcgtgcctct ctggggcgac cggtcagcga
     3721 tggatggtgc gctcacactt tccgggagaa gttcggcgaa tggcctgacg ggctgagcag
     3781 ttttccgatg gaaatcaccc cggaggtcag taattacatc agacacaaat tcatccggtt
     3841 tgccagaggg cgtagccggg cagagaagat gacggaaaaa tcccccgaag cgctttctct
     3901 gtccctgctt catggtgtcc ggtctggggt gccggaaggc agtgagtcgt ggcagatcat
     3961 gcaggcaaag caacaacttc agaaaaacag aaacagtctg agtcagtaag atgaaaacag
     4021 cagaagcagc gaaaggccac tggcctgaaa ttttaaaaca ttacggactg ccgccagtca
     4081 cgggtaaaaa gcacttcaaa ggggaatgtc cggtttgtgg tgccagaggt aagttccgta
     4141 ttgatgaccg tgatggcacc ggaacgtgga tctgtgtttg tggtagcggt gacggcatga
     4201 aactgatcgc tctgacacaa aaaagagcgt ttcatgaaat ttgcgctgaa atagaccgca
     4261 tcacggggaa tgaataccgg agagataaac cgcctgtgat ctgtacgtcg gaaagcctga
     4321 gatcgcgcat tcagcgtcga ttttcagcgc tcacgtcgct acagggaaca tctggtgctg
     4381 aatatcttcg ttcccgtggc atattcagtc ttccggttga gggagttcgc ttcaacagcc
     4441 agcaaaatta taacgggcgc atgttccagt ctctttatgc cctggcgacc gatgacaagg
     4501 gagaactgtg ttacctgcat cagacattgc ttgatggagc taaaaaagca gacattggta
     4561 tcagcgccag aaggctcaaa tctttacagg acgataacta tctggaccat gcccggtcag
     4621 ttgctatcag gatgtttccg gtagccagta cactgggtat agctgaaggt atcgaaaccg
     4681 cgttgtccgc acatcagatt tacaaagtaa atacctgggc aaccattaac agtggtttta
     4741 tgaaaaagtt ccgcgtaccg gcgggagtca cccatctgat tatttttgcc gatcgcgatg
     4801 aatacagtgc taccggaatg gctgcggcct gtgaatgcgc acatgcaaat ctccaggccc
     4861 gaaatgacat tcagcgggta agtgtccggt ggcctgatca tgatgatttc aacaatatgc
     4921 tcatgaacgg cgatcaggtt cgggaactgg ttttttacag aaaaaaggcg gttgcgtgat
     4981 gcgtacggac aatcaggtac ataaagcttt attcactatc ccgacggcag cgtacagcgc
     5041 cgttccggca aatatcaaac ctctgccaga acaaaggagg atcaccggac ataaacagac
     5101 tgatgcttat ctctggattc tggaggttat tcacctgaac gaggccgtac atctggacgc
     5161 agccgaagca gcactggaga aactcaaaat aacgccggaa gaagcgagtg aacggtatgg
     5221 acgttatttg caggagatta atgttgatcc tttccagatt gctttcgcaa ccataggcat
     5281 ggataacccg gcgcaggcga tcaggaatgc ccgtgagaat atcaaaaaat cctcatcagt
     5341 cagggccaca tttggcagct atgaggcagc actcgacgat gtggaggctg agcggataat
     5401 tcgcacctcc ccgaaattta tcgacgatta ctactggggc tggactgcgg cagagaaaaa
     5461 agccggaagt attgacggtg ttcgctcaaa tgaaattgat gatcagcgtc gtgcatatgt
     5521 tgatggctat cgtgatgtac tgccagagcc tcatacattg tcagacgttg ttcgtgagtt
     5581 tatttactgg gactggcttt atgagatgcg ccagacagcg gggagagaaa caggggacaa
     5641 atacggtttc acaggtgaac atcatgagtc agtatatgat cggcagttct ggctcgaaaa
     5701 tttgctggga aaaataaagc ctgtgacgcg tgatgaagcg gttgaagtgt gtcgctggtt
     5761 tctggcaagc ggaaaagatg agtacatgga agacaatggt tcagcggtca ttctcaatct
     5821 ggttggggag tgtgagcaat gaagccggag atatccagta gacattttcc ccctgagcat
     5881 tcaaatcaga aaaagtatgg ttacagcgga gtacagaata atgcgtgata tccaacaggt
     5941 tctgtcccga tggggtgcat gggtggtaaa taatcatgag agtgtggcat ggtccggcgt
     6001 cgctgccgga tttaagggag tgatcccatc aaaggttaaa tcacggcctc agtgtaccga
     6061 tgatgatgcg ctgattatca gcaattgcat ggcgcaactg aacgtcaaca acagcgattt
     6121 gcatgatttt ctgtatgatt attatgtgtt cgggatgacg cttatgggac tgggccgtaa
     6181 gcatgagcgc tctgattgct gggccgggcg ggtactgcaa aaagctgaag gtgttattga
     6241 ggggatgttg attatgcagg gaataaaact ggaaatggac agatacgttg agcgtgaacg
     6301 atcaagggca caggtcagtc agttttccgg acatacggga aattgaaagc gcggggtttt
     6361 actgtagaat gactgcgggt gcttgagggg gtctgcctcg ggcatgctgc cgtaaggcag
     6421 acggagaaaa gccccagtta acattacgtg tcctgcaaga cgcttaacat taatctgagg
     6481 cccaatctat gtcacacaac cgtaggttag cctcttacgt gccggaaggc aaggagaagc
     6541 agtctatgaa gcagcaaaag gcgatgttaa ttcgccctga tcgtcatctg tttaaccgtc
     6601 atagtgacgg cactggtaac gaggaaagac ctctgcgagg tacggatccg aaccggccag
     6661 acggaggtcg ctgtcttcgt agactacgaa tctgagaagt aagagaccag gcgggggagt
     6721 aatctcccgc cacctctgat gtgtcaggca tcctcaacgc acccgtactt tatttgtcat
     6781 atgctccgca aaatgcaggt tgtcgttgca acccgtgcgg taattttttg tgtgcgagtg
     6841 ctcaaaaaac gttgattttc ataaaaggat gattttatgc tgcc
//
LOCUS       DAZFVO020000219         6879 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFVO020000219 DAZFVO020000000
VERSION     DAZFVO020000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.221"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..221
                     /locus_tag="ACHKNB_RS23115"
                     /old_locus_tag="pgaptmp_004623"
     CDS             <1..221
                     /locus_tag="ACHKNB_RS23115"
                     /old_locus_tag="pgaptmp_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4198000.1"
                     /translation="GKLPEAFFWTDADNNDVPMDAETLIALSAAAEQAMFTKGLEIHI
                     RQRTMKKEIEALGDAEAILAYKVGMADR"
     gene            1028..2290
                     /locus_tag="ACHKNB_RS23120"
                     /old_locus_tag="pgaptmp_004624"
     CDS             1028..2290
                     /locus_tag="ACHKNB_RS23120"
                     /old_locus_tag="pgaptmp_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4198001.1"
                     /translation="MSKKFTKTILSSVVAGLMLVSSGAMAVDYDITVYADGAAKITER
                     GKSTEVLAAINTTTGSVMAFNNEEFLKGAEKILKKHNLNNGRMTAEARAEIQAFLEDI
                     AYPVYMPELKLENITTLSSSDIEKVKKVKDDISKVITSANSAEYLNAVKSGGDTEAYL
                     AIQQTSPALSKEYSRISGNIEQLNKDTTFAIDADGNITLDETAGTGERYGVKDVVADV
                     VADTTVYAEEDGSLTMDNSAPARKRVNEALVDLDKSVRERTTVGVSSDGTLTRAEGAK
                     NTISVNDGLVALSGRTDRIDAAVGAIDGRVTRNTQSIEKNSKAIAANTRTLQQHSARL
                     DSQQRQINENHKEMKRAAAQSAALTGLFQPYSVGKFNATAAVGGYSDQQALAVGVGYR
                     FNEQTAAKAGVAFSDGDASWNVGVNFEF"
     gene            2379..2852
                     /locus_tag="ACHKNB_RS23125"
                     /old_locus_tag="pgaptmp_004625"
     CDS             2379..2852
                     /locus_tag="ACHKNB_RS23125"
                     /old_locus_tag="pgaptmp_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198002.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIICDTLLGRLMRCMFQGRHFLQLGAESLRDGEQISDAIRNGVWTYNSVARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKQIACAE"
     gene            2857..3030
                     /locus_tag="ACHKNB_RS23130"
                     /old_locus_tag="pgaptmp_004626"
     CDS             2857..3030
                     /locus_tag="ACHKNB_RS23130"
                     /old_locus_tag="pgaptmp_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198003.1"
                     /translation="MLPDLQRLQVIQGQMMNKDKCRIYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIMQ"
     gene            complement(3067..3315)
                     /locus_tag="ACHKNB_RS23135"
                     /old_locus_tag="pgaptmp_004627"
     CDS             complement(3067..3315)
                     /locus_tag="ACHKNB_RS23135"
                     /old_locus_tag="pgaptmp_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4198004.1"
                     /translation="MRVEICIAKEKISKMPKGSVSALQQEMLRRVSKRYDDVDVIVRA
                     ANNDSLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            3545..3613
                     /locus_tag="ACHKNB_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /pseudo
     CDS             3545..3613
                     /locus_tag="ACHKNB_RS23140"
                     /old_locus_tag="pgaptmp_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyco3, capsid size determination Sid domain
                     protein"
     gene            3610..4110
                     /locus_tag="ACHKNB_RS23145"
                     /old_locus_tag="pgaptmp_004629"
     CDS             3610..4110
                     /locus_tag="ACHKNB_RS23145"
                     /old_locus_tag="pgaptmp_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HGU4198005.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAERLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            4184..4756
                     /locus_tag="ACHKNB_RS23150"
                     /old_locus_tag="pgaptmp_004630"
     CDS             4184..4756
                     /locus_tag="ACHKNB_RS23150"
                     /old_locus_tag="pgaptmp_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HGU4198006.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDVA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            5167..5568
                     /locus_tag="ACHKNB_RS23155"
                     /old_locus_tag="pgaptmp_004631"
     CDS             5167..5568
                     /locus_tag="ACHKNB_RS23155"
                     /old_locus_tag="pgaptmp_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein gop"
                     /protein_id="HGU4198007.1"
                     /translation="MLNHVYGLIGVVGTIITIATSFIRNQDISQWLLVCSGWLAALLI
                     GWFTHRTIKAISNNHTNVIKSNMEVIKSHNESNQNLMAENKELIKELARTSEQKEKME
                     SIAAYLATQNPQINAMPRTASRPENIDSEAN"
     gene            5571..6638
                     /locus_tag="ACHKNB_RS23160"
                     /old_locus_tag="pgaptmp_004632"
     CDS             5571..6638
                     /locus_tag="ACHKNB_RS23160"
                     /old_locus_tag="pgaptmp_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HGU4198008.1"
                     /translation="MKVYFENYSYYPALRTRSAEMTGLNNLSYENKKKILPLISLGKW
                     PRSEEIQVSLDKSLEVMSNLPFILDVTKDNSHHCASSFELLSPENGFKNWIEFCSRND
                     NIIPVVQMPDSAKLRDISIQARVLEELKGSIAFRIRNLNTDINKTLTSLVSMNSPENA
                     IVFIDLGYIRGNVSAITAAAINSINQIRTEIPEAIISVLATSFPSSVTNFCRENGQSG
                     YIDVIERELHQNIGGSDVAIYGDHGSIHSVVYDNIIGRYVPRIDIALNDSWYFERRPG
                     MNKEGFIEAAKSILAEYPHYQREDSWGAAMIRNAAIGDIAGMGSPAKWIAVRVNLHLN
                     KQIELSEALQYGFDHDEEDLI"
     gene            complement(6666..>6879)
                     /locus_tag="ACHKNB_RS23165"
                     /old_locus_tag="pgaptmp_004633"
     CDS             complement(6666..>6879)
                     /locus_tag="ACHKNB_RS23165"
                     /old_locus_tag="pgaptmp_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HGU4198009.1"
                     /translation="LHGNQEISINDLALLSKAKRRNNSLVKFEFKACSEKSLNEYHKV
                     DVDSIVRKYGDDVSYIPEKVLKRVVG"
BASE COUNT         2028 a         1373 c         1671 g         1807 t
ORIGIN      
        1 cggggaaact gccggaggcg tttttctgga cggatgcgga caataatgac gtaccgatgg
       61 acgctgaaac gctgattgct ctgagtgctg ctgcagagca ggcgatgttt accaaaggtc
      121 tggaaattca tatcagacag cgcacgatga aaaaagagat tgaagcgctg ggtgatgcgg
      181 aagctatact ggcgtataag gtcggcatgg ctgacaggtg aaaaaaagag gggcgctcca
      241 gccccttgct acaaagctat ggctaaataa agaatactca ggtcgaggaa ataatctggc
      301 aatgataatt aaaggagatg caacggcctc cttcacccct gtcaaagata aaaaactggg
      361 gagtgcgtgc tccccgagag catgatactt gtggttgata gttattttta tccggcgaca
      421 aagatattga gatgagggcg cggatgcaat acagaatttt cgtattgcat ggtaactgct
      481 gattcctgaa actgaatgtt gcccggcatc tgtaagtgaa aaacggggag acgacagacc
      541 cagaaattat acatgagata gtgctgcttt tttataagca acagaatgac tgcataaaaa
      601 tagagcaaat aaaacaatca tcaaatacag aattttcgta ggctgaggga ttgctgaaaa
      661 taatttagtg cgtccttcaa tggtcattca atggtcatca gattgtgctg atgtaaacag
      721 ttaagcacaa tttgattatt tgactttctt ctttttgtga ctaacctcac aattaaagat
      781 gtaaccgtat acaaattgtt gatgaataaa gagacagcat aagtggcagg ccagtcgtaa
      841 tgcggatatc ccttcgcgga cgtgatatca ccgacgttat agtaaggaaa ttccgaaaaa
      901 ctttatatta aaattcggaa tttcttcagg agtaagctca tgagagcggc gagacatcag
      961 attttcttta tatatccgat gttgaactgt tttatttgta tacagataat tttatagggt
     1021 ataccacatg agcaagaaat ttacaaagac tattctttca tctgttgttg ccggactgat
     1081 gctggtgagt tctggggcta tggctgttga ttatgatatc actgtatatg cggatggcgc
     1141 tgcaaaaata actgaaaggg ggaagtcaac agaagtctta gctgcgatta atactacaac
     1201 aggtagtgtt atggcattta ataatgagga gtttttaaaa ggtgcggaaa aaattttaaa
     1261 aaaacacaat ttaaataatg gccgcatgac agctgaggct cgcgcagaaa tacaagcttt
     1321 tctcgaggat attgcatatc ctgtgtatat gcctgaattg aaattagaga atataactac
     1381 attgagtagt agtgatatcg agaaagtaaa aaaagtgaaa gatgatatct ctaaagttat
     1441 tacatctgct aactcagcag aatatttgaa tgctgtaaaa agtggaggtg atactgaggc
     1501 ttatttagca attcaacaaa cctcgccagc tttatcaaaa gaatactctc gcatttccgg
     1561 gaatatcgaa caactgaata aagacaccac ttttgcaatt gacgctgacg gcaacattac
     1621 tctggatgag acagccggta caggtgagcg ctatggtgtg aaggatgttg tggctgatgt
     1681 tgttgctgac acgactgtgt atgccgaaga agatggctca ctgacgatgg ataacagtgc
     1741 tccagcaaga aagcgtgtta atgaagcact ggttgacctg gacaaatctg ttcgtgagcg
     1801 taccactgtt ggtgtgagtt ctgatggcac cctgacccgt gcagaaggtg caaagaacac
     1861 catttctgtt aacgatggtc tggtggccct gtctggtcgt actgaccgta ttgatgcagc
     1921 agttggtgcc attgatggcc gtgtcacccg taacacgcaa tccattgaga aaaacagcaa
     1981 ggcgattgca gccaataccc gtacgctgca gcaacattca gcacgtctgg acagccagca
     2041 gcgccagatt aatgaaaacc ataaggaaat gaaacgagca gcagcacagt ctgcggccct
     2101 gacaggtctg ttccagccgt acagtgtggg taaattcaac gccacggcag cggttggtgg
     2161 ttacagcgac cagcaggctc tggcagtggg tgtgggttac cgcttcaacg agcagaccgc
     2221 agcgaaagca ggcgtggcat tcagtgatgg cgatgcctcc tggaacgttg gtgtgaactt
     2281 cgagttttaa ttgctgaagg tgacatgaag tatgacggca ggcgcaggac tctggtctgc
     2341 cgtttttcat ggggcataaa aaacaggaag gtgctgcgat gaaggtgatt ctggctacca
     2401 ggaatcgcta tctggagtat ggtctgcagg cgttactgaa ggaacacagt gtgatactgg
     2461 cgagggagtt tttcctgccg gagaaccgcc gttatatccc ggactttgac gaatcctggc
     2521 tgataatctg tgatacgttg ctgggcagac tgatgcgctg catgttccag ggacggcatt
     2581 ttctgcaact gggtgcggaa tcactccgtg atggtgagca gataagtgat gccatacgca
     2641 atggtgtatg gacgtataac agcgttgccc gtccgctgac gatgtcagag atggtggtga
     2701 tgtttggcta cgtctaccgt cagtcaagac cctgtcgcct tgccagtgaa atgggtattc
     2761 acacgaagac ggtgaatacc tttctgtata cgggaatggc gaaaaacggg ctgtatggtg
     2821 tgagcgtaaa gcagattgcc tgtgcggagt gattcagtgt taccggacct gcagcgtctt
     2881 caggtgattc aggggcagat gatgaataaa gacaaatgcc gtatttacgg gagaaaacgt
     2941 tatacagcgg gagcaatgtg cttcatgctg tttgtgtggt tgggtggtgt ggtgacactc
     3001 tgtgccgttg ctgcaattat catgcaataa aaaatgggga gtaagttaac tccccgtaaa
     3061 gaagatttac cgaataaacc agtcgtcagc ggtttcccag gcttcctgca gtgtttcctg
     3121 aacaaattcg tgcgcggatt ctttatctac agctcttaaa atggtgagac tgtcattgtt
     3181 ggcggctctg acaatcacgt ccacatcgtc ataacgttta ctgacacgtc gaagcatttc
     3241 ttgctgcagg gcagaaacag aaccttttgg cattttgctg attttttctt tagcgataca
     3301 gatctcaaca cgcataacac cctcctgtaa ctgtggttat gtacaggtgt tatttttgtc
     3361 tgtatggata accagtgtca attcctgata ttgtttatgg ggcatcaatg gggcatgtat
     3421 gggacacttt ttatcggcga aattcgtcga agttcgtcga catggtaaac gaatcatcta
     3481 tccaaccctt gaaaaacggc gctcctggac gatcttcgtc gatttttaaa aatgttgcgt
     3541 cacgcgcaag gtgtggcagg acaaaataga ccagcaggga gcagtgctta aggccagagg
     3601 actgctgtca tgatttactg cccgtcgtgt ggacatgttg ctcacacccg tcgcgcacat
     3661 ttcatggacg atggcaccaa gataatgatt gcacagtgcc ggaatattta ttgctctgcg
     3721 acatttgaag cgagtgaaag ctttttctct gactgtaaag attcaggaat ggaatacatt
     3781 tcaggcaaac agagataccg cgattcactg acgtcagcct ccggtagcat gaaacgcccg
     3841 aaaagaatgc ttgttaccgg atattgttgt cggagatgta aaggccttgc actgtcaaga
     3901 acatcgcggc gtctgtctca ggaagtcacc gagcgttttt atgtgtgcac ggatccgggc
     3961 tgtggtctgg tgtttaaaac gcttcagacc atcaaccgct tcattgtccg cccggtcaca
     4021 ccggacgaac tggcagaacg cctgcatgaa aaacaggaac tgccgccagt acggttaaaa
     4081 acacaatcat attcgctgcg tctggaatga gggctaccgg ttaaccccgg ccgtcgccgc
     4141 acaccgtatt tttattcttc agcatgatga gaaagagata acgatggaaa gcacagcctt
     4201 acagcaggcc tttgacacct gtcagaataa caaagcagca tggctgcaac gcaaaaatga
     4261 gctggcagcg gccgaacagg aatatctgcg gcttctgtca ggagaaggca gaaatgtcag
     4321 ccgcctggac gaattacgca atattatcga agtcagaaaa tggcaggtga atcaggccgc
     4381 cggtcgttat attcgttcgc atgaagccgt tcagcacatc agcatccgcg accggctgaa
     4441 tgattttatg cagcagcacg gcacagcact ggcggccgca ctggcaccgg agctgatggg
     4501 ctacagtgag ctgacggcca ttgcccgaaa ctgtgccata cagcgtgcca cagatgccct
     4561 gcgtgaagcc cttctgtcct ggcttgcgaa gggtgaaaaa attaattatt ccgcacagga
     4621 tagcgacatt ttaacgacca tcggattcag gcctgacgtg gcttcggtgg atgacagccg
     4681 tgaaaaattc acccctgcgc agaacatgat tttttcgcgt aaaagtgcgc aactggcatc
     4741 acgtcagtca gtgtaaaatt ccccgaaaat ccgcccgttt ttactgaaaa aagccatgca
     4801 tcgataaggt gcatggcttt gcatgcgttt tcctgcctca ttttctgcaa accgcgccat
     4861 tcccggcgcg gtctgagcgt gtcagtgcaa ctgcattaaa accgccccgc aaagcgggcg
     4921 ggcgaggcgg ggaaagcacc gcgcgcaaac cgacaagtta gttaattatt tgtgtagtca
     4981 aagtgccttc agtacatacc tcgttaatac attggagcat aatgaagaaa atctatggcc
     5041 tatggtccaa aactgtcttt tttgatggca ctatcctgaa aaatatgcaa aaaatagatt
     5101 gatgtaaggt ggttcttatc agtgtcgcaa gatccttaag aattcgtggc atgagagagt
     5161 taaaggatgc tgaatcatgt atatggatta attggcgttg ttgggaccat aatcactatt
     5221 gctactagct tcatccgcaa ccaagatatt tcacaatggt tgctcgtttg ttctggatgg
     5281 ttagctgctt tacttattgg atggtttaca cacagaacga ttaaagcaat tagcaacaac
     5341 cacacgaatg ttataaaaag taatatggag gtaatcaaga gtcataatga atccaatcaa
     5401 aacttgatgg ctgaaaacaa agaattgatt aaagaacttg caagaacttc agaacagaaa
     5461 gagaagatgg aaagtatagc tgcttatctt gctactcaga acccccaaat taacgctatg
     5521 ccaagaacag caagccgacc agaaaatatc gattcggagg ctaattaaat atgaaagtat
     5581 attttgaaaa ttactcatac tatccagccc taagaacacg atcagcagaa atgaccggac
     5641 tgaataattt atcatatgag aataagaaaa aaatattacc tttaatttct ttggggaaat
     5701 ggcctcgctc ggaagaaata caggtatcgt tagataaaag tttagaagtg atgagtaatc
     5761 tcccatttat tttggatgtc acaaaggata attctcatca ttgcgcgtca agcttcgaac
     5821 tactctctcc tgagaatggt tttaaaaact ggatagagtt ttgttccaga aatgataata
     5881 ttattccagt agtccagatg ccggattcag caaaacttag agatatatct atccaagcca
     5941 gggttttaga ggaacttaaa ggttcaattg catttagaat tagaaatcta aatacagaca
     6001 ttaataaaac cctgacgtca ctagtatcga tgaactctcc agaaaatgcc atagttttca
     6061 tagatttggg ctacattaga gggaatgtat ctgctataac agctgcagca ataaattcga
     6121 ttaatcagat tagaactgag attcctgaag ccataattag tgtgctagca acaagcttcc
     6181 ctagttcagt tacaaatttt tgccgtgaaa atggacagtc cggatatatt gatgttattg
     6241 agagagaatt gcatcaaaat attggtggaa gtgatgtcgc tatttatggc gatcatggtt
     6301 caattcattc agtagtgtat gacaacataa ttgggcgata tgttcctaga attgatatag
     6361 cgttaaatga ttcatggtat ttcgagcgcc gccctgggat gaataaagaa gggttcattg
     6421 aagctgcgaa atcaattttg gcagagtatc cacattatca aagggaggac tcttggggag
     6481 ctgcaatgat aagaaatgct gctatagggg atatagcagg tatgggaagt ccagctaaat
     6541 ggattgctgt tcgagtgaat ctacacttaa ataagcaaat tgagctatct gaagctctac
     6601 agtatggctt tgatcatgat gaggaagatc ttatctaata aaaaactagg gaaggttagc
     6661 cttccctagc ccacaaccct tttaagtact ttttccggaa tataggaaac atcatcgcca
     6721 tatttcctaa ctatagagtc aacatcaacc ttatggtatt catttaaaga tttctccgaa
     6781 caagccttaa actcaaactt aaccaaagaa ttattccttc ttttagcttt gctcaataag
     6841 gcaagatcgt ttatagatat ttcttgatta ccatggagt
//
LOCUS       DAZFVO020000220         6835 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFVO020000220 DAZFVO020000000
VERSION     DAZFVO020000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.222"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..688
                     /locus_tag="ACHKNB_RS23170"
                     /old_locus_tag="pgaptmp_004634"
     CDS             <1..688
                     /locus_tag="ACHKNB_RS23170"
                     /old_locus_tag="pgaptmp_004634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198010.1"
                     /translation="WLADSRTRGLADSRTRGLADSRTRGLADSRTRGLISYELTPQEY
                     NAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNADRIPGSLPFVTAGEGNEGISAFIS
                     NDVVVFPENTITIDMFGSAKYRNYEYGADDHVAVVHTEFFDRNAVIFITASIHKSSCT
                     GKFNYGRNFYAKDADELMVSLPSKDNNPDYSFMETLILAVHKLVIKDVVQYTDKKLVA
                     YRQITSEKVV"
     gene            1528..3567
                     /locus_tag="ACHKNB_RS23175"
                     /old_locus_tag="pgaptmp_004635"
     CDS             1528..3567
                     /locus_tag="ACHKNB_RS23175"
                     /old_locus_tag="pgaptmp_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4198011.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLHHPNADLSKGQYGC
                     VGQGLHIAKKLLAFIPKNAGILLVPCCRGGSAFTSGAEGAFSESSGATAESSLWGVGK
                     PLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFLNMVNQFRADLS
                     AYSSQCTGGNASFVPWICGDTTYLWKQKYTSQYPVVYGAYQNKEAQKIYFVPLMTDEK
                     GTNTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRGIVSDRLATAIL
                     MHAGKTAAFLKGTPAPFSVVVAPPATAGSDSATTESPQPSTSAPQPGAGTQPSPEVPP
                     ATETPARTTAVAYRADTQEGKLKEQGWTGADGKASVVDDSAAESGKAMRVEKVQGQQT
                     AWKIAHDVPASKAAELLQRGGEIAFRFKIPEGVQLATNRFAMALYWPVSQLPENVVLE
                     GSAGNDMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSISVT
                     PVINGQTQAAFNLAKCPAAGLDNDKLLLTDITSNQDTYPVLIEHVTVAINNSNAA"
     gene            3713..3895
                     /locus_tag="ACHKNB_RS23180"
                     /old_locus_tag="pgaptmp_004636"
     CDS             3713..3895
                     /locus_tag="ACHKNB_RS23180"
                     /old_locus_tag="pgaptmp_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4198012.1"
                     /translation="MSFIHQIMLYFCTAVCVLYLLSGGDRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            3921..4193
                     /locus_tag="ACHKNB_RS23185"
                     /old_locus_tag="pgaptmp_004637"
     CDS             3921..4193
                     /locus_tag="ACHKNB_RS23185"
                     /old_locus_tag="pgaptmp_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4198013.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
     gene            4270..4485
                     /locus_tag="ACHKNB_RS23190"
                     /old_locus_tag="pgaptmp_004638"
     CDS             4270..4485
                     /locus_tag="ACHKNB_RS23190"
                     /old_locus_tag="pgaptmp_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4198014.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            4490..5023
                     /locus_tag="ACHKNB_RS23195"
                     /old_locus_tag="pgaptmp_004639"
     CDS             4490..5023
                     /locus_tag="ACHKNB_RS23195"
                     /old_locus_tag="pgaptmp_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4198015.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDHVNAIERDKALAWVEKNIRVPLTDPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5245..5353
                     /locus_tag="ACHKNB_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /pseudo
     CDS             5245..5353
                     /locus_tag="ACHKNB_RS23200"
                     /old_locus_tag="pgaptmp_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5575..5760
                     /locus_tag="ACHKNB_RS23205"
                     /old_locus_tag="pgaptmp_004641"
     CDS             5575..5760
                     /locus_tag="ACHKNB_RS23205"
                     /old_locus_tag="pgaptmp_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4198016.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPG
                     PDWQTPLNGIISSSENG"
     gene            5999..6589
                     /locus_tag="ACHKNB_RS23210"
                     /old_locus_tag="pgaptmp_004642"
     CDS             5999..6589
                     /locus_tag="ACHKNB_RS23210"
                     /old_locus_tag="pgaptmp_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4198017.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIGYPDLIKAFQPDIAMRRIVAVFPH"
BASE COUNT         1800 a         1594 c         1831 g         1610 t
ORIGIN      
        1 ctggttagcg gactcgcgga ctcgcggact cgcggactcg cggactcgcg gactcgcgga
       61 ctcgcggact cgcggactcg cggactcgcg gactcgcgga ctgataagct atgagcttac
      121 accacaggaa tataacgcga ttgagctatt aaatagcaat aatcttatgt ggggcactta
      181 taacctcaaa aagttatttg gccccgcaat aagaggtaag cgtctgaaaa acgcagatcg
      241 tatacccggc tccttgcctt ttgttactgc gggtgaaggc aatgaaggta tttctgcatt
      301 tatcagtaat gatgttgttg tattccctga aaataccatt acgatagata tgtttggctc
      361 cgcaaaatac agaaattatg agtatggtgc agatgaccat gtggcagttg ttcatacaga
      421 attctttgac agaaatgccg ttattttcat aacagcatca attcataagt cttcttgtac
      481 cggaaagttc aactacggga gaaatttcta tgcaaaagat gccgacgagt taatggtatc
      541 tttgccgtca aaagataata accctgatta ttctttcatg gagacgctaa ttctagcagt
      601 acataaactg gtcatcaaag atgttgtcca gtacactgat aaaaagctgg tagcgtacag
      661 gcagattaca tctgagaagg ttgtttagtg caaatcccca cctgacaggg tggggaatgg
      721 tcaggttatc gtgccctgat gcagaaaaac agtttacaat cgtaaaaatc atcatatcat
      781 gacaaaagtg gttacattgc cacgctgctt aacccgccga tgcgcgggtt ttttgtatcc
      841 agaatcctgt aagttatacg gaaagtacac agaaaggaag gtgcgaccgc aattaataac
      901 aaaatcttaa aaatcgcata tggcactatt agttttctaa atattgtata ttttaagtat
      961 tgcaggatga ccctgtaacg aagtttgcgt aacagcattt tgctctacga gtttgccagc
     1021 ctcccccagt ggctggcttt ttttgtatcc gttcaacagg aatgttacat acctcacaat
     1081 taagtctgtt gaatgttgtc tgcccggatg agaatttgtt agaaaaaaac tgcatggtga
     1141 atccccctgt gcggaggggc gactggtgaa tggtatgatc tctttgatga tcgtaagcga
     1201 gaatacgcgg gtttggtggc accaggccga actcaccggg aggcacccgg caccatgcag
     1261 tatacagaga ttaggcatat atcagggccc ctcatagcag gggccttttt acatgcaaaa
     1321 aaaagccgcc ccgggaagag cggttggcaa gaagaaaagc aatatgaaca attaattaac
     1381 gctgcgaata ataccttaca gtaatcaccc tgcgcaactg tgaggagtgt atttcttttt
     1441 tgcgggtggt tgtctttttc ccttgtgttt cgggacttcc gctcactcct tatcttattc
     1501 agtacattat cccggccggg aggattcatg gcatttaaac actatgacgt ggtcagggcg
     1561 gcgtcgccgt cagaccttgc ggaacgaata actcaaaaac tgaaggaagg gtggcagcct
     1621 tatggtagtg cgctgatttc gacagctggt tatggtgcgg agttcatcca gccagttgtg
     1681 agtgagggga gcatctcatc accagaggag ccaggcaacc gtccgacgac ctcagcgcct
     1741 tctgttgcgc cagaatatta ctatgtgatc gcgcttgctg gtcagtccaa tggtatgtca
     1801 tacggtgagg gactgccatt accggataca ttcgacagcc ctgatccacg tattaaacag
     1861 ttagcgcgtc gcagtacggt gacaccgggc ggcgctgcat gtacctataa cgatattatt
     1921 ccggcagatc actgtctcca tgatgtacag gatatgagca gccttcatca tccgaacgcc
     1981 gatctgtcaa aaggtcagta tggctgcgtc ggacagggtt tacatatcgc caaaaaatta
     2041 ctggcgttta tcccgaaaaa tgccggaatc cttctggtac cttgctgtcg tggagggtca
     2101 gcatttactt ccggcgctga gggggcattc agtgaatcgt caggagcgac ggccgaatca
     2161 tccctgtggg gtgtcggcaa gccgctgtac aacgatctgc ttacccgtac aaaagccgcg
     2221 ctggataaaa accccaaaaa cgtcctgtta tccgtggtct ggatgctggg agagtttgat
     2281 ctgaagtcag caacatacgc acagcacccg caattattcc tgaacatggt gaatcagttc
     2341 cgcgccgatc tcagcgcata ttcttcacag tgcaccggag gcaatgcatc atttgttccc
     2401 tggatttgcg gcgataccac ctatctctgg aaacaaaaat atacctctca gtacccggtg
     2461 gtttacggtg catatcagaa caaagaagcg caaaaaattt attttgtgcc cctgatgacc
     2521 gatgagaaag gtactaacac gccaacaaac ctgcctgccg aagatcctga tctggcagaa
     2581 gccggttatt acggtgctgc atcccgcagt aatggaaact ggacctcttc agatcgcgcc
     2641 acgcatttca gttcatgggc ccgtcgcggc attgtttctg accgtctggc aacggcgatc
     2701 ctgatgcatg cagggaagac agccgccttc ctgaaaggca cacccgcacc gttcagcgtg
     2761 gtcgtagcgc ctcccgccac ggcaggttca gacagcgcca ccacggaaag cccgcagccc
     2821 tccacatctg cacctcagcc gggcgcaggc acacagccat caccggaagt tccaccggca
     2881 actgaaacac cggcaagaac aactgccgtc gcttatcgtg cagatacaca ggaagggaaa
     2941 ctgaaagaac agggctggac gggggcagac ggaaaagcct ctgtcgtaga tgattctgct
     3001 gctgagagcg gtaaagccat gcgagtggaa aaagtccagg ggcaacaaac agcctggaaa
     3061 attgcccacg atgtgcctgc aagtaaagca gctgaattac ttcagagagg cggtgaaatt
     3121 gccttccgtt tcaaaattcc cgaaggtgtt cagctggcaa caaaccgctt cgccatggcc
     3181 ctgtactggc ctgtttctca gctacctgaa aacgttgttc tggaaggcag cgcaggaaac
     3241 gatatgctgg ccgccttctt catccagagc gacagcgcaa acctgaatct gatgcagcac
     3301 aaacaacaga atgcaaaact gggaaccttc ggggcattta atcataactg gcacaacgtg
     3361 gtgttcaggt ttgccggcaa taacagcatc agcgtgacac cggtcatcaa cggtcagacc
     3421 caggcggcct ttaatctggc gaaatgcccg gcggcaggac tggataacga caagctgtta
     3481 ctcactgaca ttaccagtaa ccaggatacc tacccggttc tgattgagca cgtaacagtg
     3541 gcaatcaata acagcaacgc cgcataagaa aaaagaggct cccggcaggc aagactgaga
     3601 ggaatagata ccagccggga gcgtaagagg aattatggag agtgtttatt attgttattc
     3661 atttttttaa aacgccagac atcgttatgt caatcatttg tgtgaggtga ttatgtcatt
     3721 cattcatcaa atcatgctgt atttctgtac ggcggtctgt gtgctgtatc ttctttcggg
     3781 tggggacagg gctgtgcgcg atttctggtg caggcagatt gataaaaggg ccgctgagaa
     3841 aatcagcgcc agtcagtcag ccggaagcaa acccgaagag cccgttactc cttaacaacc
     3901 cctttcagcg agaaaatccc atgtcagaaa tcacatccct ggtcactgca gaggcagtga
     3961 aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa acagaaactc cgccagaatc
     4021 ttgaggcgcg tcttgatgca gaagttgatg ccattctgga tgagctgctt ggtgtacagg
     4081 cagagccacc gactgaagcg ggagatacca ccgcagagag cggtgaagtt cagcctgaat
     4141 caccggtcgc cgatgcgact gaacctcaac ccgaatcggt catgatgttg taacggggag
     4201 tcagggccat cagtaaacag ctgctggcct ttttcatgtt gtgagcttcc ggattgcggg
     4261 agacggggta tgtaccagat ggaaaaaatc acaacaggtg tgtcatacac cacgtcagcg
     4321 gtgggaacgg gctactggtt cctgcagttg ctggacaggg tttccccgtc tcagtgggcg
     4381 gcaataggcg tgctggggag tctgctgttt ggtctgctga cgtacctgac gaacctgtat
     4441 ttcaaaatca gagaggacag gcgtaaggcg gcacggggag agtaagctga tgagcaggaa
     4501 actccgctat ggtttatcgg ctgccgttct ggcgctgatt gccgcaggtg cttctgcgcc
     4561 tgaaatcctc gaccagtttc tggatgaaaa ggaaggtaac cacaccacgg cataccgtga
     4621 tggtgcgggt atctggacca tctgccgtgg agccacccgg gtggatggta agcctgtgat
     4681 tcctggcatg aagctgtcga aggaaaaatg cgaccatgtt aacgccattg aacgtgataa
     4741 ggcgctggca tgggtggaga aaaacatcag agtgccgctg accgaccccc agaaagcggg
     4801 gatagcgtca ttctgtccgt ataacattgg ccccggtaag tgtttcccgt cgacgtttta
     4861 taaacgaatt aatgcaggtg atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa
     4921 ggacggtggc agagactgcc gtatccgctc aaataattgc tacggtcagg tatcccggcg
     4981 agatcaggaa agtgcgctgg cgtgctgggg aattgacaga taagcagaat attttgctga
     5041 aaaatgacat tggccaacgc gggcggataa cacgaaatcc tgcgaactgg caaaacctaa
     5101 gtgaataaaa gtaaaaaccc cggttgttgg cagcaagcgg ggtttggttt tttctgacct
     5161 tgaataaggc aagggagaaa ttatgggtag ggaggtactt tccctgtgag gaagtataaa
     5221 agattctttc tgaggttgtc cattatgaaa ggcattgaag tggagagcca gtctggattt
     5281 aacaagagcg gcagcttttg ccattcgtat tgtggccatt gctgttctgg tctgggcaat
     5341 ccgttggtgg tgatatgaac cgtgttctgt gcgtggtcat cattgtcctg ctggtggcct
     5401 gtggtgcgct tagtctgggg ctgaatcatt accgtgttca cgccatcatc tacaaagagc
     5461 agcgcgataa aaaagccagt gagctggagc tggcgaacgc gacaattact gatatgcaga
     5521 tacgccagcg tgatgtcgct gcacttgatg ccagatactc gagggaatta gccgatgcga
     5581 gagctgaaaa tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca
     5641 acgccacctg ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa
     5701 ccaggccccg actggcagac accgctgaac gggattattt catcctcaga gaacggttga
     5761 tgacaatgca gaagcagctg gaaggggcac aggaatatat ccgcactcag tgcctgaatt
     5821 aacagagcca gcttaatcgc tggctttttc atatctgaat ttcatcgcgc atctcacgcg
     5881 catatcacat ccccgagcct ttcagaaagt tgagcctgag aactgtcgta tatggtggcg
     5941 accatctcgg ggcggctttt ctgtgagaca ggctcaattt tctgaaaggt aagacgctat
     6001 gaactatccg acgattgtta acggtattga tttccgcgat cttgtttttg tgacaggcaa
     6061 cgagccggtg acggataccc gaaaggttgc cgtagcattt ggcaaagaac acaaagatgt
     6121 attgcggaaa acaagagccg ttgttcaaca atgctcaaaa gaatttgcag agcgcaattt
     6181 tgcgctttgc tatgaaaaca atgagttaca gaacggtaaa ccccagccca tatatcgaat
     6241 gacaaaagac ggttggacga tgttggtatt tggttttacg gggaaagctg ctatgacctt
     6301 taaggaagcg tacattcagg ctttcaactg gatggctgag cttattcagc aaggtctggc
     6361 gaatctggag gcggagcgca acgctgtaat gctggagtac atgaaagaga aagatgtcgc
     6421 cagtatgtca ggtcgtttgc tcaatcgctg gggcagggtg aagaagcctc agttgctggc
     6481 aaggctggac aggctggagc agcaggggca gattggttac ccggatttga taaaggcatt
     6541 tcagcctgac atagccatgc gccgtatcgt cgccgtattc ccgcattaac agagaccgca
     6601 gccaccttat ctgcgtgagt gtgcgggggt aatcaaaaac gatgcacacc gggttttctc
     6661 atttttcacg agatgggagc gatttcccgc gaagccgcct gtccggtgcg gtggtggaag
     6721 aaaccggata aaacaacctc attgtacaaa tatcgatcaa gtatggcgct gctgtgtgaa
     6781 ttctgaaaaa tcacagcggt cattatgcgt cagtttttaa cagaggacgt cagaa
//
LOCUS       DAZFVO020000221         6817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFVO020000221 DAZFVO020000000
VERSION     DAZFVO020000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.223"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(205..690)
                     /gene="sixA"
                     /locus_tag="ACHKNB_RS23215"
                     /old_locus_tag="pgaptmp_004643"
     CDS             complement(205..690)
                     /gene="sixA"
                     /locus_tag="ACHKNB_RS23215"
                     /old_locus_tag="pgaptmp_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HGU4198018.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(896..3037)
                     /gene="fadJ"
                     /locus_tag="ACHKNB_RS23220"
                     /old_locus_tag="pgaptmp_004644"
     CDS             complement(896..3037)
                     /gene="fadJ"
                     /locus_tag="ACHKNB_RS23220"
                     /old_locus_tag="pgaptmp_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HGU4198019.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPALLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            complement(3037..4347)
                     /gene="fadI"
                     /locus_tag="ACHKNB_RS23225"
                     /old_locus_tag="pgaptmp_004645"
     CDS             complement(3037..4347)
                     /gene="fadI"
                     /locus_tag="ACHKNB_RS23225"
                     /old_locus_tag="pgaptmp_004645"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HGU4198020.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4527..4811)
                     /locus_tag="ACHKNB_RS23230"
                     /old_locus_tag="pgaptmp_004646"
     CDS             complement(4527..4811)
                     /locus_tag="ACHKNB_RS23230"
                     /old_locus_tag="pgaptmp_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HGU4198021.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5183..6523
                     /gene="fadL"
                     /locus_tag="ACHKNB_RS23235"
                     /old_locus_tag="pgaptmp_004647"
     CDS             5183..6523
                     /gene="fadL"
                     /locus_tag="ACHKNB_RS23235"
                     /old_locus_tag="pgaptmp_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HGU4198022.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1705 a         1837 c         1617 g         1658 t
ORIGIN      
        1 tgtcaaacag acattattca tttcaacatg taactttggg caaaatcaag atacgcgcag
       61 aagaataatt tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga
      121 tagtagtgag gttgacagat atccgctgcc tgatgagcaa ttatgcccaa tgaatctacc
      181 tcattgggca taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct
      241 catctgccag ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga
      301 ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa
      361 cggtaagtga gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata
      421 ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga
      481 aggcaggttc agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct
      541 caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga
      601 ttcgtcacaa ccattagtgg tcagaggacg aacggaatca ctggcggcat cgagggctgc
      661 gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc
      721 cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat
      781 tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac
      841 attttgacca acactccgcc attcagcgcg gattcatata gctttgacct tcttattaca
      901 ggtcagttgc agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct
      961 cgcaaggggt aaaacgggaa ccatactgcg tggcaagtcg ttgcattatt gcaaccactt
     1021 cgcccgcgcc gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa
     1081 tgccaaatac cgcgccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac
     1141 aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga
     1201 gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc
     1261 ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat
     1321 cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg
     1381 cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga
     1441 ttgggcctac cggaaaacca aatttcacta gcgcagcatc aatgtgctct acccgttcac
     1501 cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa
     1561 acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg
     1621 tagtggcgat ggtttgcgct gatgtacccg catgaggaat aatctccacc agcggcattt
     1681 tttccaccgg actgaagaaa tgcaggccga taacttgctc aggtcgcgtg gcgtgagcgg
     1741 cgatatcacc aatcggcaaa gatgacgtat tcgaagcaaa gatggtatga gcggcgcaat
     1801 tttgctccac ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa
     1861 taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct
     1921 gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc
     1981 aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa
     2041 gccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa
     2101 ttcccacgct gtttaatagc gcaggcggtg catcactacc gggatctttc ttcacttccg
     2161 tactggcaaa aaagatacta cgcaaagctt gcgattgtgg cgtcatcgcc agttcgccaa
     2221 acgcccgagc ttcggcgtca taaccgctgc tggtgccctg cgctaatccc gtttcaacaa
     2281 cctccaggat gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttgc
     2341 cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag
     2401 gtagagggcg ggaagatggg cgatccttct tcgccagctc aacagcgact tccagcaaaa
     2461 tggagtgcgg aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt
     2521 gttttccggt gaggatcatc tctaatgctg tgctgacgcc tatcagacgc ggtaaacgct
     2581 gggtgccgcc tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt
     2641 taggatcgtc agtacaaacg cgaccgtggc acgccagcgc caactccagc ccaccaccca
     2701 ggcaagcgcc atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca
     2761 actgttggcc ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccgatca
     2821 tgttgatgtc tgcgccagca atgaagttgt ccggtttagc ggagacaaac accacgcctc
     2881 gcaactcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaactccg
     2941 ccttcagggt attcattttc tcacccggta cgtcgatggt gatgatagca atgttgtcca
     3001 gacgaacatt aagagtaaac actgatgcca tttccattat tccgcctcca gaaccattgc
     3061 cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg
     3121 aagttcatgc aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgcccgta
     3181 agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc
     3241 acgccccagt acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt
     3301 ctgagctgca aaggcttcgt gcatatcgat caatgtcaga tcgctcattg tcaaaccggc
     3361 acgttccagc gccagcggtg ttgaccaggc cggaccgagc aacatgtcct gccagacatc
     3421 aatcgcagta aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc
     3481 ccgggattca gtcatcagga tcaccgccgc tgcgccatcg gtcagcggcg tactgttcgc
     3541 cgccgttacc gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag
     3601 cgaggaatta ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa
     3661 ggcagtcatc acctcttctt tgagtttacc gtctgaccac gcctgagcgg cacgctgatg
     3721 cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg
     3781 ttcggcggtg tcgcccatcc gcaagccagt agaatattct gctaccgcag gcggtacggg
     3841 cattaagtcg cgcaaacgca ggcgagagaa gagtttcagt cgctggctca tggttcgagc
     3901 tttgttgaca tcaaccagca cgcgcgccag ttttttactg acgccaattg gcaatacgga
     3961 agaggaatct gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc
     4021 gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt
     4081 atgcacattc attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat
     4141 ttgtacgacc tgaccaaaga ccagctgttc aatcacttca gcggggatct cgctgcgtgc
     4201 cagcagttcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt
     4261 cgcctggcgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg
     4321 ggtaaccagc ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt
     4381 ctgacctgat catagtctta accatttttt tacatttagc caagcggaga aaaaggaaag
     4441 tgggagctat gacacagtga agacaggaga aagaaaagaa aacgcccctg ccagcaactg
     4501 acaggggcga acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca
     4561 gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa
     4621 gtcggggaga ttttgcacgg ttcggattcc acgctacggg ctttttcagt cagcgccgcc
     4681 agcgtttgtt ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg
     4741 gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta
     4801 catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg
     4861 atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg
     4921 accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa
     4981 cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac
     5041 atacttgcaa cattccagct ggtccgacct atactctcgc cactggtctg atttctaaga
     5101 tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa
     5161 ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc
     5221 agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt
     5281 ttcttcctct ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg
     5341 taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg
     5401 tgcggtttat attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct
     5461 gaaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat
     5521 taacgaccaa tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt
     5581 taatgatact tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct
     5641 gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc
     5701 cgtctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg
     5761 tcagattatg caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc
     5821 caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg
     5881 ctggaacgcc ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg
     5941 ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa
     6001 taactacggt ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac
     6061 gctgaacctg cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc
     6121 gattcactat agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc
     6181 aaccagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc
     6241 gttgggtacc acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga
     6301 tgacagccca gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg
     6361 gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc
     6421 ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg
     6481 taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacacg ttcgcctgga
     6541 taaagtcacc tgcatagcag gtgactttaa ctcccccact tcaccgaaag tagtgtcctc
     6601 attgcttacc tcctgagttt tgcaaacagc ctgatggcag cttgcccttt caaatcaata
     6661 agcggtatcc acagacaaat ctgaagggaa aggcatattt tcaggcgttc tgctcgtcct
     6721 tctcaaagag tttacttttc tgaatttcca ggatactccc cccccctggc tattgtgcgc
     6781 tcatacactc aaattaaaga taggttctaa ataaatg
//
LOCUS       DAZFVO020000222         6744 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFVO020000222 DAZFVO020000000
VERSION     DAZFVO020000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.224"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..205
                     /locus_tag="ACHKNB_RS23240"
                     /old_locus_tag="pgaptmp_004648"
     CDS             <1..205
                     /locus_tag="ACHKNB_RS23240"
                     /old_locus_tag="pgaptmp_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGU4198023.1"
                     /translation="KKICRAEGATEEDDNKLVREFERLTEHPDGSDLIYYPRDDREDS
                     PEGIVKEIKEWRAANGKSGFKQG"
     gene            267..410
                     /locus_tag="ACHKNB_RS23245"
                     /old_locus_tag="pgaptmp_004649"
     CDS             267..410
                     /locus_tag="ACHKNB_RS23245"
                     /old_locus_tag="pgaptmp_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HGU4198024.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            905..1819
                     /locus_tag="ACHKNB_RS23250"
                     /old_locus_tag="pgaptmp_004650"
     CDS             905..1819
                     /locus_tag="ACHKNB_RS23250"
                     /old_locus_tag="pgaptmp_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4198025.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVAT
                     SARTLKPWEKLGISRAWYYQLKKRGLVE"
     gene            complement(2044..2310)
                     /locus_tag="ACHKNB_RS23255"
                     /old_locus_tag="pgaptmp_004651"
     CDS             complement(2044..2310)
                     /locus_tag="ACHKNB_RS23255"
                     /old_locus_tag="pgaptmp_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HGU4198026.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHKNB_RS23260"
                     /old_locus_tag="pgaptmp_004652"
     CDS             2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHKNB_RS23260"
                     /old_locus_tag="pgaptmp_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HGU4198027.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4941..6686
                     /gene="col"
                     /locus_tag="ACHKNB_RS23265"
                     /old_locus_tag="pgaptmp_004653"
     CDS             4941..6686
                     /gene="col"
                     /locus_tag="ACHKNB_RS23265"
                     /old_locus_tag="pgaptmp_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HGU4198028.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEV
                     SKDPDLSKQFKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNI
                     RVTTPKRHIDIHRGK"
BASE COUNT         1732 a         1554 c         1900 g         1558 t
ORIGIN      
        1 aaaaaaaata tgtagagctg aaggtgctac tgaagaggat gacaataaat tagtgagaga
       61 gtttgagcga ttaactgagc acccagatgg ttcagatctg atttattatc ctcgcgatga
      121 cagggaagat agtcctgaag ggattgtcaa ggaaattaaa gaatggcgag ctgctaacgg
      181 taagtcagga tttaaacagg gctgaaatat gaatgccggt tgtttatgga tgaatggctg
      241 gcattctttc acaacaagga gtcgttatga aaaaaataac agggattatt ttattgcttc
      301 ttgcagtcat tattctgtct gcatgtcagg caaactatat ccgggatgtt cagggcggga
      361 ccgtatctcc gtcatcaaca gctgaagtga ccggattagc aacgcagtaa cccgaaatcc
      421 tctttgacaa aaacaaagcg tgtcaggctg attctgatgc gctttttttt gaaatgtcac
      481 aaaaattcca tgtgggagat gggatctaaa atccccgtgc agaactttcc atccaggggg
      541 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg
      601 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
      661 ctggtgtttc cttgtctttt tgcgtttttt gcgtcagaaa gcgattgagg gcgttttaag
      721 gggtacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
      781 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt cgtgtctctc
      841 cgccaagatt gtcacgagaa tacaggggcg aggtcggtcg ataacctgga aggaatcgta
      901 acacatgagc gccgcgcttc aatacttcga agaaaattta ccccaccgtc cctatcacac
      961 ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc ttgcgcggta
     1021 catccagcag aaccagcctc atgcgcagtt ctggctggtt tttgacgttg atcgcgaggg
     1081 ggctgcgatt gactggagcg accggaacgc ccccgcgccg aacatcaccg taaaaaatcc
     1141 cgtgaacggt catgctcacc tgctctatgc actcaacatc gccgtgagaa ccgcgcctga
     1201 tgcgtcggtt aaggcactga agtacgctgc cgcgatagag cgtgcgctgt gtgagaaact
     1261 tggtgcagat gtgaactaca gcggactgat ttgcaaaaat ccgttccacc tggaatggct
     1321 ggtgatggag tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt
     1381 gagcgcctca gcgcgccgta gcatcgataa acattacggg atggggcgaa actgctacct
     1441 gttcgaaatg acgcgcaaat gggcttacag ggcgattcgt cagggctggc ctgtattctc
     1501 acaatggctt gatgccgtga tccagcgtgt cgaaatgtac aacgcatctc ttcccgttcc
     1561 gctttctccg gctgaatgtc gggctattgg caagagcata gcgaaataca cgcacaggaa
     1621 ctttacaccg gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc
     1681 tacacgcggt cgcaagggcg gttctaagtc taagcgcggc acagtagcta catcagcacg
     1741 cacgctgaaa ccttgggaga aattagggat cagtcgcgcc tggtattacc aactgaaaaa
     1801 acgaggtctc gtagagtaga ccaaataagc ctatatcaga taacagcgcc tttttggcgc
     1861 ttttttgagc agcttggttt gttgctattt ccctcgttga atcccgcaat ggcgcggctt
     1921 tccgcatgat tgaggtggta gcgctcgccg cagtctcatg accgagcgta gcgagcgaat
     1981 gagcgaggaa gcgcaaaggt gtcggtggtg catgtggcac ttacgcgccg gggtttctcc
     2041 gttctacggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca gcggttgtat
     2101 ccggttcaca tcggcctgtc ttgtgacttc ctttcgcagg aactggagca ggaacgcacg
     2161 cagttgcgct tcttccggcc tccgtaccct cgccagcatg gcagccccca caatgacttt
     2221 ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg cctcagcttc
     2281 ggcaatcttc tgttcgagtg ttctgctcat ttcgtgactc cgtgcgcggt gaaaaatcgc
     2341 attttagcgc gtcactggta gttgcaaaac tgaactggca taatgcacgg cacatcacga
     2401 agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg acgctaaaag
     2461 aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca tgaaaatcat
     2521 ttcgcgaaaa aacggctaca gtgccgttgc ttctgctgcc taccgttccg gctctgtcat
     2581 acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg ttgatgatgc
     2641 ggtcattctc acccctgcga atgcaccgtc ttggtgtggt gaccgttccg ttctctggaa
     2701 tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga ttgaactcgc
     2761 cattccccgt gagatttccc gcgaggccgc acgggaggcc gttctcgctt ttgtccggga
     2821 aaactttgtc agtcggggca tgattgccga tgtggcgttt catcacatgg accggaccaa
     2881 tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacaggat ttgcaggaaa
     2941 ggtcagggac tggaacgagc gggcactcgc cgagacgtgg cgcgcatcat gggctgacca
     3001 tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc gttcatacga
     3061 gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct gtgcgatgga
     3121 aaaacggggc atggaaacag aacgcggtga gcagaaccgc ctgattaaca gccttaacct
     3181 ggaaatacag gtttcccgca caaggctggc agtcatccag gtacaggaga acgtgcggaa
     3241 acgggaactc agcgatgccg cacgtcgtgc agcggaagcc cttaacctga ccattcccgc
     3301 tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgctgccgc aggaatgcgg
     3361 gaacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga acgacatcga
     3421 acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga tgaccggact
     3481 caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga catgggctga
     3541 accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga ttgaatctct
     3601 tcgccgcacc tacagggccg tgaaagaacg ggacattccc gcccgtcgtc aggcctttga
     3661 aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa aactgtcagc
     3721 acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga aggaacagga
     3781 gcatgaggcg cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa cggcattagt
     3841 cggggttatt acggagctgg ggcgtgccag agagccggga acgggcagga taacccgcta
     3901 catgatgttg agtaacagag ccggagaatt cacggtatgg ggtgatgagc tggcgcatta
     3961 cccccagagt gttcatgacc cggtgaatgt ttacctgtcg ccaggcgggg ctgtgatggt
     4021 ctcggatata cgcgagggaa tgccagaatc tcatgagacg atggcgcggc ctgagcgtgt
     4081 gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc aggggtggcc
     4141 ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt tcagcgacga
     4201 ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca ccgctgatgc
     4261 tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca ggacgctgaa
     4321 tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc gggaaggagg
     4381 gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag tcagcaccat
     4441 cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg atgaggatac
     4501 tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc atgacaggct
     4561 tttggctgct gtccataaac cggaagagat gccggattat ctcttccgtc aactcggttt
     4621 taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac aacgccgccc
     4681 cgggcacttc cggggcatga gtatgtgata tccggggctg caccccggac cccgccaaca
     4741 catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt tatccctcct
     4801 gatttctaaa aaattttccg cctgaacttg acagaaaaaa cgatgacgag tactttttga
     4861 tctgtacata aaaccagtgg ttttatgtac agtattaatc atgtaattaa ttgttttaac
     4921 gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac acgggcgcgc
     4981 atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt ggtggtgctt
     5041 ctgatggctc cggatggagt tcggaaaata acccgtgggg tggtggttcc ggtagcggca
     5101 ttcactgggg tggtggttcc ggtcatggta atggcggggg gaatggtaat tccggtggtg
     5161 gttcgggaac aggcggtaat ctgtcagcag tagctgcgcc agtggcattt ggttttccgg
     5221 cactttccac tcctggagct ggcggtctgg cggtcagtat ttcagcggga gcattatcgg
     5281 cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt ctttgggggg
     5341 tggctttata tggtgtactg ccatcacaaa tagcgaaaga tgaccccaat atgatgtcaa
     5401 agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt tcattacctc
     5461 tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa gacgaacgac
     5521 agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat gcaaaaccta
     5581 ccgaacgtcc aggtgttttt acggcatcaa ttccaggtgc acctgttctg aatatttcag
     5641 ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat aatactgata
     5701 aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca gttattcgat
     5761 tcccgaagga cagcggtcat aatgccgtat atgtttcagt gagtgatgtt cttagtcctg
     5821 accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg gatgctacgc
     5881 atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg aatcaggcaa
     5941 atgaagatgt tgccagaaat caggagcgac aggccaaagc tgttcaggtt tataattcgc
     6001 gtaaaagcga acttgatgca gcgaataaaa ctcttgctga tgcaatagct gaaataaaac
     6061 aatttgaacg atttgcacat gacccaatgg ctggcggtca cagaatgtgg caaatggccg
     6121 ggcttaaagc tcagcgggcg cagacggatg taaataataa gcaggctgca tttgatgctg
     6181 ctgcaaaaga gaaggcagat gctgatgctg cattaagtgc cgcgcaggag cgccgcaaac
     6241 agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt aaacggaata
     6301 agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg gatgatgcag
     6361 gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt gataaagaat
     6421 ttaaaaactt tgacgatttc cggaagaaat tctgggaaga agtgtcaaaa gatcccgatc
     6481 ttagtaagca atttaaaggc agtaataaga cgaacattca aaagggaaaa gcaccttttg
     6541 caaggaagaa agaccaagta ggtggtaggg aacgctttga attacatcat gataaaccaa
     6601 tcagtcagga tggtggtgtc tatgatatga ataatatcag agtgaccaca cctaagcgac
     6661 atattgatat tcatcggggt aagtaaaaat ggaactgaaa catagtatta gtgattatac
     6721 cgaggctgaa tttctggagt ttgt
//
LOCUS       DAZFVO020000223         6680 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFVO020000223 DAZFVO020000000
VERSION     DAZFVO020000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.225"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            80..856
                     /gene="exuR"
                     /locus_tag="ACHKNB_RS23270"
                     /old_locus_tag="pgaptmp_004654"
     CDS             80..856
                     /gene="exuR"
                     /locus_tag="ACHKNB_RS23270"
                     /old_locus_tag="pgaptmp_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HGU4198029.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1096..1179
                     /gene="yqiM"
                     /locus_tag="ACHKNB_RS23275"
                     /old_locus_tag="pgaptmp_004655"
     CDS             1096..1179
                     /gene="yqiM"
                     /locus_tag="ACHKNB_RS23275"
                     /old_locus_tag="pgaptmp_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HGU4198030.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1201..1863
                     /gene="yqjA"
                     /locus_tag="ACHKNB_RS23280"
                     /old_locus_tag="pgaptmp_004656"
     CDS             1201..1863
                     /gene="yqjA"
                     /locus_tag="ACHKNB_RS23280"
                     /old_locus_tag="pgaptmp_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HGU4198031.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1867..2250
                     /gene="mzrA"
                     /locus_tag="ACHKNB_RS23285"
                     /old_locus_tag="pgaptmp_004657"
     CDS             1867..2250
                     /gene="mzrA"
                     /locus_tag="ACHKNB_RS23285"
                     /old_locus_tag="pgaptmp_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HGU4198032.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2397..2765
                     /gene="yqjC"
                     /locus_tag="ACHKNB_RS23290"
                     /old_locus_tag="pgaptmp_004658"
     CDS             2397..2765
                     /gene="yqjC"
                     /locus_tag="ACHKNB_RS23290"
                     /old_locus_tag="pgaptmp_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HGU4198033.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2803..3108
                     /gene="yqjD"
                     /locus_tag="ACHKNB_RS23295"
                     /old_locus_tag="pgaptmp_004659"
     CDS             2803..3108
                     /gene="yqjD"
                     /locus_tag="ACHKNB_RS23295"
                     /old_locus_tag="pgaptmp_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HGU4198034.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3111..3515
                     /gene="yqjE"
                     /locus_tag="ACHKNB_RS23300"
                     /old_locus_tag="pgaptmp_004660"
     CDS             3111..3515
                     /gene="yqjE"
                     /locus_tag="ACHKNB_RS23300"
                     /old_locus_tag="pgaptmp_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGU4198035.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3505..3804
                     /gene="yqjK"
                     /locus_tag="ACHKNB_RS23305"
                     /old_locus_tag="pgaptmp_004661"
     CDS             3505..3804
                     /gene="yqjK"
                     /locus_tag="ACHKNB_RS23305"
                     /old_locus_tag="pgaptmp_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HGU4198036.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3990..4382
                     /gene="yqjF"
                     /locus_tag="ACHKNB_RS23310"
                     /old_locus_tag="pgaptmp_004662"
     CDS             3990..4382
                     /gene="yqjF"
                     /locus_tag="ACHKNB_RS23310"
                     /old_locus_tag="pgaptmp_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HGU4198037.1"
                     /translation="MKKLEGVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4452..5438
                     /gene="yqjG"
                     /locus_tag="ACHKNB_RS23315"
                     /old_locus_tag="pgaptmp_004663"
     CDS             4452..5438
                     /gene="yqjG"
                     /locus_tag="ACHKNB_RS23315"
                     /old_locus_tag="pgaptmp_004663"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HGU4198038.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5731..6096
                     /gene="yhaH"
                     /locus_tag="ACHKNB_RS23320"
                     /old_locus_tag="pgaptmp_004664"
     CDS             5731..6096
                     /gene="yhaH"
                     /locus_tag="ACHKNB_RS23320"
                     /old_locus_tag="pgaptmp_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4198039.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6338..>6680
                     /locus_tag="ACHKNB_RS23325"
                     /old_locus_tag="pgaptmp_004665"
     CDS             6338..>6680
                     /locus_tag="ACHKNB_RS23325"
                     /old_locus_tag="pgaptmp_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HGU4198040.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPK"
BASE COUNT         1685 a         1617 c         1645 g         1733 t
ORIGIN      
        1 aatttatata tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat
       61 tccactccgg gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc
      121 cgctgacctg aaagagcgca tcgaacaggg cgtctatctg gtaggtgata aactgcctgc
      181 agaacgcttt attgccgatg aaaagaacgt cagccgtacg gtagttcgtg aagccatcat
      241 catgctggaa gttgaaggct atgtcgaagt gcgtaaaggt tcaggtattc atgtggtttc
      301 caaccagccg cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc
      361 gtttgagttg cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac
      421 tcaggtaacg aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga
      481 acaatgcttc cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac
      541 gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg acccaacgta gccataaccc
      601 gtactggaaa aaactgcacg aacacattga ttcccgtacc gtcgataact ggtgtgatga
      661 ccacgatcaa atcctcaagg cgctgattcg caaagatcct catgctgcta agctggcaat
      721 gtggcagcac ctggaaaaca ccaagatcat gttattcaac gaaaccagcg acgacttcga
      781 gttcaatgcc gaccgctatc tgttcgccga aaacccggta gtgcatctcg atacggccac
      841 cagcggcagt aaatgaaata cctgtccgac aggcgctgcg acgcgcctgt ttgtctcgtc
      901 ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat
      961 tcagcaaaat cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt
     1021 acaattagat tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag
     1081 ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac
     1141 attgcggtag tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga
     1201 atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc
     1261 aatccatcga tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac
     1321 ggcttgcttc cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg
     1381 attgcgaaag gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc
     1441 ctcggctgct gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa
     1501 aactggctat ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa
     1561 cacggtttat cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg
     1621 acgattgccg ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc
     1681 ggtctgctgt gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta
     1741 tttttaaagt acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg
     1801 gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg
     1861 taagggatgc aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta
     1921 cttctggtcg gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg
     1981 gcgattcgtg ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac
     2041 cttgacgctc atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt
     2101 acatttgact ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc
     2161 catggctaca tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg
     2221 ttacgggata actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact
     2281 ttggtgattt caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg
     2341 ttgtctggac aatcgttcct ttgtaatagg tccgaatcac aatggaaggt tcaagaatga
     2401 aataccgcat cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta
     2461 ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc
     2521 aaaaccagaa tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt
     2581 cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg
     2641 cggaacgcca gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca
     2701 aacgcgaacg gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact
     2761 actaactcac aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact
     2821 acggaacatc tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc
     2881 tcatctggcg agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca
     2941 ctgaaacaga gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt
     3001 gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggcatt
     3061 ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca
     3121 ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca
     3181 tcatggttga aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa
     3241 aagctaatct ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg
     3301 gtcttatgag cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg
     3361 cgatgattgc caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt
     3421 tacgtaaatc gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc
     3481 ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg
     3541 caactgctta gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg
     3601 ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg
     3661 gcgctggttg gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc
     3721 cgctgggcca gacgcggttt tggcgtatgg agcgcctggc gtctggttaa aacgaccctc
     3781 aagcagcaac agcttcgcgg ttaacttccc tctggccgga gcaattccgg ccttatccct
     3841 caaatttttt gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta
     3901 tgattctctc catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca
     3961 gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt agaaggtgtt ggtgtactgg
     4021 tagcgcgcat tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt
     4081 actctggtac ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg
     4141 tgattctgct tgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca
     4201 cagccctgtt tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg
     4261 ctgaaggcgt caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc
     4321 tgctggcaat taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt
     4381 aagcacgcgc tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg
     4441 aggagagaaa aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca
     4501 aatctaccgg cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg
     4561 atggcgcacc tggccccact ggcacaggcg gttttatcgc agagaaagat cgttatcatc
     4621 tctatatttc actcgcctgc ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag
     4681 gactggaacc gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga
     4741 cctttgatga cagttttccg ggagcaaccg gagatacgct ctatcaacat gaatttctgt
     4801 atcagcttta tctccacgcc gatccacact acagcggacg agttactgtt cccgtgctgt
     4861 gggacaaaaa gaaccacacc atcgtcagca acgaatcagc agaaatcatc cgcatgttca
     4921 ataccgcgtt tgatgcgctg ggcgcaaaag cgggtgatta ctacccacca gccctgcaaa
     4981 cgaaaattga cgaacttaac ggctggattt atgacaccgt taacaacggc gtgtataaag
     5041 ctggttttgc caccagccag caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc
     5101 tggcgcgact ggagcagatt ttaggccagc atcgttacct gaccggcaac cagctaaccg
     5161 aagccgatat tcgcctgtgg accacgctgg tccgttttga tccagtgtat gtgacccact
     5221 tcaagtgtga taagcaccgc atcagcgatt accttaatct gtatggcttc ctgcgtgata
     5281 tctaccagat gccgggaatt gccgaaacag tcaatttcga tcacatccgt aatcattact
     5341 tccgcagcca taagaccatc aatcctacgg ggattatttc aattggtcca tggcaggatc
     5401 tcgatgaacc gcatggacga gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc
     5461 ctctgataat tagctaagtg catgttgttc atgtcggatg cggcgtgaac gccttatcaa
     5521 acctacaaaa catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc
     5581 gcatctggcg attttgcgct tgtcatcagt cttaacgcgc cttcacagca ctttattaat
     5641 tccttgaacg aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa
     5701 aacaagtgat tgagcaaaat tgaggcaaaa atggactggt atctgaaagt actaaaaaat
     5761 tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac
     5821 atcatcttta cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt
     5881 ggcgaaggca tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt
     5941 cagttccgcc gcctgcacga caccgaccgc tcggcgtggt gggcactgtt gttcttaatc
     6001 ccgtttatcg gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa
     6061 aaccgctttg gaccagatcc gaaactggaa ccttaaaaca aatctgctcg ccggatgtct
     6121 gactcatccg gcttttatca tcatgttgat gaatgaataa tttactaatg tttatttaaa
     6181 atatttcaat gtcgttatat aaattaccta taaaaaacaa ccatgtattt atcttaaata
     6241 tcaaaacctt tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga
     6301 atagactttc gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt
     6361 aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct
     6421 gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt
     6481 tccattcctt tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt
     6541 tcgtcgtctg cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat
     6601 cattggctgg ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg
     6661 ttacggaaac gatcctaagt
//
LOCUS       DAZFVO020000224         6482 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFVO020000224 DAZFVO020000000
VERSION     DAZFVO020000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.226"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            159..1418
                     /gene="codB"
                     /locus_tag="ACHKNB_RS23330"
                     /old_locus_tag="pgaptmp_004666"
     CDS             159..1418
                     /gene="codB"
                     /locus_tag="ACHKNB_RS23330"
                     /old_locus_tag="pgaptmp_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HGU4198041.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1408..2691
                     /gene="codA"
                     /locus_tag="ACHKNB_RS23335"
                     /old_locus_tag="pgaptmp_004667"
     CDS             1408..2691
                     /gene="codA"
                     /locus_tag="ACHKNB_RS23335"
                     /old_locus_tag="pgaptmp_004667"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HGU4198042.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(3028..3927)
                     /gene="cynR"
                     /locus_tag="ACHKNB_RS23340"
                     /old_locus_tag="pgaptmp_004668"
     CDS             complement(3028..3927)
                     /gene="cynR"
                     /locus_tag="ACHKNB_RS23340"
                     /old_locus_tag="pgaptmp_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HGU4198043.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            4037..4696
                     /gene="cynT"
                     /locus_tag="ACHKNB_RS23345"
                     /old_locus_tag="pgaptmp_004669"
     CDS             4037..4696
                     /gene="cynT"
                     /locus_tag="ACHKNB_RS23345"
                     /old_locus_tag="pgaptmp_004669"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HGU4198044.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4727..5197
                     /gene="cynS"
                     /locus_tag="ACHKNB_RS23350"
                     /old_locus_tag="pgaptmp_004670"
     CDS             4727..5197
                     /gene="cynS"
                     /locus_tag="ACHKNB_RS23350"
                     /old_locus_tag="pgaptmp_004670"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HGU4198045.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5203..6384
                     /gene="cynX"
                     /locus_tag="ACHKNB_RS23355"
                     /old_locus_tag="pgaptmp_004671"
     CDS             5203..6384
                     /gene="cynX"
                     /locus_tag="ACHKNB_RS23355"
                     /old_locus_tag="pgaptmp_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HGU4198046.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPARVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
BASE COUNT         1345 a         1719 c         1807 g         1611 t
ORIGIN      
        1 cactacccac tgtttttaca ccgataattt ttcccccacc tttttgcact cattcatata
       61 aaaaatatat ttccccacga aaacgattgc tttttatctt cagatgaata gaatgcggcg
      121 gattttttgg gtttcaaaca gcaaaaaggg ggaatttcgt gtcgcaagat aacaacttta
      181 gccaggggcc agtcccgcag tcggcgcgga aaggggtatt ggcattgacg ttcgtcatgc
      241 tgggattaac cttcttttcc gccagtatgt ggaccggcgg cactctcgga accggtctta
      301 gctatcatga tttcttcctc gcagttctca tcggtaatct tctcctcggt atttacactt
      361 catttctcgg ttacattggc gcaaaaaccg gcctgaccac tcatcttctt gctcgcttct
      421 cgtttggtgt taaaggctca tggctgcctt cactgctact gggcggaact caggttggct
      481 ggtttggcgt cggtgtggcg atgtttgcca ttccggtggg taaggcaacc gggctggata
      541 ttaatttgct gattgccgtt tccggtttac tgatgaccgt caccgtcttt tttggcattt
      601 cggcgctgac ggttctttcg gtgattgcgg ttccggctat cgcctgcctg ggcggttatt
      661 ccgtgtggct ggctgttaac ggcatgggcg gcctggacgc attaaaagcg gtcgttcccg
      721 cacaaccgtt agatttcaat gtcgcgctgg cgctggttgt ggggtcattt atcagtgcgg
      781 gtacgctcac cgctgacttt gtccggtttg gtcgcaatgc caaactggcg gtgctggtgg
      841 cgatggtggc ctttttcctc ggcaactcgt tgatgtttat tttcggtgca gcgggcgctg
      901 cggcactggg catggcggat atctctgatg tgatgattgc tcagggcctg ctgctgcctg
      961 cgattgtggt gctggggctg aatatctgga ccaccaacga taacgcactc tatgcgtcgg
     1021 gtttaggttt cgccaacatt accgggatgt cgagcaaaac cctttcggta atcaacggta
     1081 ttatcggtac ggtctgcgca ttatggctgt ataacaattt tgtcggctgg ttgaccttcc
     1141 tttcggcagc tattcctcca gtgggtggcg tgatcatcgc cgactatctg atgaaccgtc
     1201 gccgctatga gcactttgcg accacgcgta tgatgagtgt caattgggtg gcgattctgg
     1261 cggtcgcctt ggggattgct gcaggccact ggttaccggg aattgttccg gtcaacgcgg
     1321 tattaggtgg cgcgctgagc tatctgatcc ttaacccgat tttgaatcgt aaaacgacag
     1381 cagcaatgac gcatgtggag gctaacagtg tcgaataacg ctttacaaac aattattaac
     1441 gcccggttac caggcgaaga ggggctgtgg cagattcatc tgcaggacgg aaaaatcagc
     1501 gccattgatg cgcaatccgg cgtgatgccc ataactgaaa acagcctgga tgccgaacaa
     1561 ggtttagtta taccgccgtt tgtggagccg catattcacc tggacaccac gcaaaccgcc
     1621 ggacaaccga actggaatca gtccggcacg ctgtttgaag gcattgaacg ctgggccgaa
     1681 cgcaaagcgt tattaaccca tgacgatgtg aaacaacgcg cctggcaaac gctgaaatgg
     1741 cagattgcca acggcattca gcatgtgcgt acccatgtcg atgtttcgga tgcaacgctg
     1801 actgcgctga aagcaatgct ggaagtgaag caggaagtcg cgccgtggat tgatctgcaa
     1861 atcgtcgcct tcccgcagga agggattttg tcgtatccca acggtgaagc gttgctggaa
     1921 gaggcgttac gcttaggggc agatgtagtg ggggcgattc cgcattttga atttacccgt
     1981 gaatacggcg tggagtcgct gcataaaacc ttcgccctgg cgcaaaaata cgaccgtctc
     2041 atcgacgttc actgtgatga gatcgatgac gagcagtcgc gctttgtcga aaccgttgct
     2101 gccctggcgc accgtgaagg catgggcgcg cgagtcaccg ccagccacac cacggcaatg
     2161 cactcctata acggggcgta tacctcacgc ctgttccgct tgctgaaaat gtccggtatt
     2221 aactttgtcg ccaacccgct ggtcaatatt catctgcaag gacgtttcga tacgtatcca
     2281 aaacgtcgcg gcatcacgcg cgttaaagag atgctggagt ccggcattaa cgtctgcttt
     2341 ggtcacgatg atgtcttcga tccgtggtat ccgctgggaa cggcgaatat gctgcaagtg
     2401 ctgcatatgg ggctgcatgt ttgccagttg atgggctacg ggcagattaa cgatggcctg
     2461 aatttaatca cccaccacag cgcaaggacg ttgaatttgc aggattacgg cattgccgcc
     2521 ggaaacagcg ccaacctgat tatcctgccg gctgaaaatg ggtttgatgc gctgcgccgt
     2581 caggttccgg tacgttattc ggtacgtggc ggcaaggtga ttgccagtac acaaccagca
     2641 caaacaacgg tgtatctgga gcagccagaa gccatcgatt acaaacggta atcggctggg
     2701 ttacagcgag cgtagtttat gccggatgcg gcgtgaacgc cttatccggc ctacgtagag
     2761 cactgaactc gtaggcctga taagcgtagc gcatcaggca attccagccg ctgatctgtg
     2821 tcagcggcta ccgtgattca tttccgccaa catccgcgca ttcctccaac gccatgtgca
     2881 aaaatgcctt cgcagcggca gtctgccagc tgtagtttat gccggatgcg gcgtgaacgc
     2941 cttatccggc ctacgtagag cactgaactc gtaggcctga taagcgtagc gcatcaggca
     3001 attccagccg cagacctgtg tcagcggcta ccgcgattca tttccgccaa caaccgcgca
     3061 tttatccaac gccatgtgca aaaatgcctt cgcggcggct gtctgccagc tatttttccg
     3121 ccgcaacaaa accgccgttc tctccagtag tggcggggca agagaaatcg ctttaagccc
     3181 gtcatgttgt gtggcaatcg ctgctggtaa caatgtggaa agggaagtgc ggcgaatcag
     3241 ctccagaacc gcgctaattg agttcgcctc aatgaccacc tgtggatgta gccccgcttt
     3301 ctcgcagtag tggtcaattt gctctctggt ggcaaattcc gcgctgagca ggaccagttt
     3361 ttcatcatgc aagcgactca acgccacctg ttcacaggca gccagcggat gatgtttcgc
     3421 caccaccaac gctaaacttt ctgtcagtaa aggaattgcc tccagctccg gcgaatgcac
     3481 aggcgcgaag gcaatcccaa cgtccagctc gtcgcgacaa agcagatctt cgattttctc
     3541 ctgcgacatt tcctgtagct ggagcgtgat gccggggtag cgcgcataga aatccgccat
     3601 taaagggccg ataaagtagc ttgtaaaggt gggggtgacg gcgatacgca gcgatcctcg
     3661 cgtcagatcg gcaacatcat gaatcgcccg tttacccgct cccagttcct gtaacgcccg
     3721 gctggcgtac tgtcgccaga cttctcctgc atcagtgaga cgaatcgttc gcccgctacg
     3781 gtcaaacagc ggcacgccta aactctcctc taactggcga atctgctggg aaagcgcagg
     3841 ctgggagacg tgcaacgcac tggcggcacg ggtaaagctg ccatgttcag ccacggcaag
     3901 aaaatagttg atatgtcgag agagcattcg ccaacctata agtaaatcca atagaactca
     3961 tcataaatga gacttttacc ttatgacaat cggcgagtag tctgcctctc attccagaga
     4021 cagacagagg ttaacggtga aagagattat tgatggattc cttaaattcc agcgcgaggc
     4081 atttccgaag cgggaagcct tatttaaaca gctggcgaca cagcaaagcc cgcgcaccct
     4141 ttttatctcc tgctccgaca gccgtctggt ccctgagctg gtgacgcaac gtgagcctgg
     4201 cgatctgttc gttattcgca acgcaggtaa tattgtccct tcctacgggc cggaacccgg
     4261 tggcgtttct gcttcggtgg agtatgccgt cgctgcgctt cgggtatctg acattgtgat
     4321 ttgtggtcat tccaactgtg gcgcgatgac cgccattgcc agctgtcagt gtatggacca
     4381 tatgcctgcc gtctcccact ggctgcgtta tgccgattca gcccgcgtcg ttaatgaggc
     4441 gcgcccgcat tccgatttac cgtcaaaagc tgcggcgatg gtacgtgaaa acgtcattgc
     4501 tcagttggct aatttgcaaa ctcatccatc ggtgcgcctg gcgctcgaag aggggcggat
     4561 cgccctgcac ggctgggtct acgacattga aagcggcagc atcgcagctt ttgacggcgc
     4621 aacccgccag tttgtgccac tggccgctaa tcctcgcgtt tgtgccatac cgctacgcca
     4681 accgaccgca gcgtaacctt atttttaaac catcaggaga tccaccatga ttcagtcaca
     4741 aattaaccgc aatattcgtc ttgatcttgc cgatgccatt ttgctcagca aagctaaaaa
     4801 agatctctca tttgccgaga ttgccgacgg taccggtctg gcagaagcct tcgtaaccgc
     4861 ggctttgctg gggcagcagg cgcttcctgc cgacgccgcc cgcctggtcg gggcgaagct
     4921 ggatctcgac gaagacgcca ttctactgtt gcagatgatt ccactgcgtg gctgcattga
     4981 tgaccgtatt ccaaccgacc caacgatgta tcgtttctat gaaatgttgc aggtgtacgg
     5041 cacaaccctg aaagcgttgg ttcatgagaa gtttggcgat ggcattatca gcgcgattaa
     5101 cttcaaactc gacgttaaga aagtggcgga cccggaaggt ggcgaacgtg cggtcatcac
     5161 cttagatggt aaatatctgc cgaccaaacc gttctgacag ccatgcgcaa ccatcaaaag
     5221 acgttcacga tgctgctggt tctggtgctg attggtctta atatgcgacc actgctcacc
     5281 tccgtcggtc cactgctacc gcaattgcgc caggcgagcg gaatgagctt tagcgtggct
     5341 gccctgttga ccgctctgcc ggtggtcacc atgggcgggc tggcgctggc cggaagctgg
     5401 cttcatcagc atgtcagcga acgtcgcagc gtcgccatca gtctgttgct gattgccgtc
     5461 ggtgcattga tgcgtgagct ttacccccaa agtgtgctgc tgcttagcag cgcactgctt
     5521 ggtggggtgg ggatcggtat cattcaggcg gtgatgcctt cagtgattaa acggcggttt
     5581 cagcaacgca cgccactggt gatggggctg tggtctgcgg ctctgatggg cggcggtgga
     5641 cttggtgccg ccataacgcc ctggttagtt caacatagcg aaacctggta tcaaacactc
     5701 gcctggtggg cgctgcctgc cgttgttgcg ctctttgcct ggtggtggca aagcgcccgc
     5761 gaggtcgcct cttcccacaa gacaacaacc actccggctc gcgtggtatt cactccccgc
     5821 gcgtggacgc tgggtgttta cttcggtctg attaacggcg gttacgccag cctgattgcc
     5881 tggttacccg ctttctatat tgagattggt gccagcgcgc agtacagcgg ttccttactg
     5941 gcattgatga cgcttgggca agccgcagga gctttgctga tgcctgctat ggctcgccat
     6001 caggatcggc gcaaactgtt aatgctggcg ctggtgttac aactggtggg gttctgcggc
     6061 tttatctggt tgccgctgca attgccggta ttgtgggcga tggtgtgtgg gttaggtctg
     6121 ggcggcgcgt ttccgctctg tttgctgctg gcgctcgatc actctgtgca accggctatt
     6181 gctggcaagc tggtggcgtt tatgcaggga atcggtttta tcatcgccgg gcttgccccg
     6241 tggttttctg gcgtgctgcg tagtatcagc ggcaattacc tgatggactg ggcatttcat
     6301 gcgctgtgcg tcgttgggct gatgatcata accctgcgtt ttgcaccagt acgttttccg
     6361 cagctgtggg tcaaagaggc atgatgcgac gcttgttcct gcgctttgtt catgccggat
     6421 gcggctaatg tagatcgctg aacttgtagg cctgataagc gcagcgtatc aggcaatttt
     6481 ta
//
LOCUS       DAZFVO020000225         6409 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFVO020000225 DAZFVO020000000
VERSION     DAZFVO020000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.227"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..823
                     /gene="rcnA"
                     /locus_tag="ACHKNB_RS23360"
                     /old_locus_tag="pgaptmp_004672"
     CDS             38..823
                     /gene="rcnA"
                     /locus_tag="ACHKNB_RS23360"
                     /old_locus_tag="pgaptmp_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HGU4198047.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHHEHGEYQDAHARAHANDIKRRFDGREVTNW
                     QILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLALTLITVGVGAAIS
                     VQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            1042..1380
                     /gene="rcnB"
                     /locus_tag="ACHKNB_RS23365"
                     /old_locus_tag="pgaptmp_004673"
     CDS             1042..1380
                     /gene="rcnB"
                     /locus_tag="ACHKNB_RS23365"
                     /old_locus_tag="pgaptmp_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HGU4198048.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1462..2496)
                     /gene="yehA"
                     /locus_tag="ACHKNB_RS23370"
                     /old_locus_tag="pgaptmp_004674"
     CDS             complement(1462..2496)
                     /gene="yehA"
                     /locus_tag="ACHKNB_RS23370"
                     /old_locus_tag="pgaptmp_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HGU4198049.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     AIALINVKYD"
     gene            complement(2510..4990)
                     /gene="yehB"
                     /locus_tag="ACHKNB_RS23375"
                     /old_locus_tag="pgaptmp_004675"
     CDS             complement(2510..4990)
                     /gene="yehB"
                     /locus_tag="ACHKNB_RS23375"
                     /old_locus_tag="pgaptmp_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HGU4198050.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQTYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(5006..5680)
                     /gene="yehC"
                     /locus_tag="ACHKNB_RS23380"
                     /old_locus_tag="pgaptmp_004676"
     CDS             complement(5006..5680)
                     /gene="yehC"
                     /locus_tag="ACHKNB_RS23380"
                     /old_locus_tag="pgaptmp_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HGU4198051.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(5761..6303)
                     /gene="yehD"
                     /locus_tag="ACHKNB_RS23385"
                     /old_locus_tag="pgaptmp_004677"
     CDS             complement(5761..6303)
                     /gene="yehD"
                     /locus_tag="ACHKNB_RS23385"
                     /old_locus_tag="pgaptmp_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198052.1"
                     /translation="MKRSIIAAAVFSYFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         1746 a         1525 c         1260 g         1878 t
ORIGIN      
        1 gaatcagatt gccgaattaa tactaagaat tattatcatg accgaattta caactcttct
       61 tcagcaagga aacgcctggt tcttcatccc cagcgccatc ttacttggtg cgcttcatgg
      121 cctggaacca gggcactcaa aaacgatgat ggcggcgttt atcatcgcca tcaaaggcac
      181 cattaaacaa gcggtgatgc tcggactggc agcaactatt tcgcataccg cagtggtctg
      241 gttaattgcc tttggcggga tggtgatcag caagcgcttt actgctcaat cagcagaacc
      301 gtggctccag ctgatttccg cagtgatcat tattggcacc gcgttctgga tgttctggcg
      361 tacctggcgc ggcgaacgca actggctgga gaatatgcac gagtatgatc atgaacatca
      421 tcaccatcac gaacatggcg agtatcagga tgcccatgca cgagcccatg ccaatgacat
      481 taaacgacgc tttgatggta gagaggtcac caactggcaa attttgttat ttggcttaac
      541 cggtggcctt atcccctgcc cggcagcaat taccgtgctg ttgatatgca ttcagttgaa
      601 agccctgaca ctgggcgcaa cactggtcgt cagtttcagc attggcctgg cgttaacgct
      661 tatcaccgta ggcgttggcg cagcaatcag cgttcagcag gtcgcaaaac gctggagcgg
      721 atttaacact ctcgctaaac gcgcccccta tttttccagt ctgttgattg gcttagtcgg
      781 tgtgtatatg ggcgtacatg gcttcatggg cataatgcga taactcaatc ctttaggctt
      841 gcatgttatc ttcagcccag gatgattctt aaatcagcta tttccgctga ctctctgctc
      901 gctaaggatg cttttagggc atcctttcat ttacactttt tacgaaatca tgggatcact
      961 aacaaaatat cgcttgtcag ttatattgta tggcaggaaa gatatgcgac tgatattaca
     1021 gatccccaaa gtggagagtt tatgaccatt aaaaataaga tgttgctggg tgcgcttttg
     1081 ctggttacca gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa tacctcggga
     1141 atttctaagt atgagttaag cagtttcatt gctgacttta agcatttcaa accaggcgat
     1201 accgtcccag agatgtaccg caccgatgag tacaacatta agcagtggca gttacgtaac
     1261 ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg gtggcgcgta cgtgttgatc
     1321 agcgacaccg acggtaaaat cattaaagcc tacgacggtg agatttttta tcatcgctaa
     1381 aaaaagcccc ctcatcatga gggggaaatg cagacacctt gttatttttt attattagcc
     1441 acttgctcgt cttgcttgtt attagtcgta tttcacgttg attaatgcga ttgcctccag
     1501 tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa ctggcagtta tcggaattga
     1561 aaccgttgta tctgtaagtt ttgactttgc cgcaatttct tttaaatttg tgtatccttt
     1621 actgctgtta gtgatattta aaagtgtgac tgacgaacca ttactttcaa gaataaatcc
     1681 tacgccatta tcttccgcac ctttcagtat tgtgttgctg ttggttaaca agtcagaact
     1741 tgataaatac acctgcattt tccgtgatgt ttgattttgt tgacctaata aatttttaca
     1801 acgcagaata atatcccctt gtttactatt tattgttgct ttgttacctg tggctggtaa
     1861 ttgtgaaacg ggaatactgt ctaaagtaat taataaatcg tccggtttgc aggttgtggt
     1921 tatcgggttg tatgacaatg tcagattaaa ggaatagaca cctgcatttt gtgccaatgc
     1981 atttctgtag ctatttacac atgcatccca tgacttgcca cctaataaac acagtagaaa
     2041 ttgtactgcg gcactcccgc gtgtcgatga tgaagcactg gataatgtcg caactccatt
     2101 aatcatcact gaaccgccgc cccccgcaga aacattcact tttgcattac tggccgcagg
     2161 tgtcaccgtt accgaataac cactggtgaa actggtctga ttatttgata acgtgatgtt
     2221 gtttttattg gtccattcaa aatgtaaatt taatttaatg gtatcgttat aaagtccaac
     2281 aataatatcc gacgggctca taggggtgat agtatttgta ctcacgcatg acgaatcatt
     2341 aaaattatgc tcatatgtcg ccatttgtgt ttgagtaagt gcaaaatccc cctgaacaac
     2401 caaattgtgc tgaacagata actcactata acatgccgca tagctcacag gcataaccat
     2461 catcattact ataaataaca ttatacgaat attcataacc acctgactat tactggcaaa
     2521 tatagtttct actttcatca atctctttac cgaaggtgat tgtgcatgaa agtccttgtt
     2581 gcttatcaat tgctacatta accgatggcg gtatttcatt ggtgcgaata aataactggc
     2641 ttccctggcc gacaacgcca atattatgac cgtggatatc attgacttca taaccaaacg
     2701 ttaatggttg cccatccgct cttaacgctt ttataaacca gggcttgcgc tgatcggtat
     2761 caaaattaac cagtacaacc gcgccgcgat aaggtgcggc aattttccgg ttgccacgta
     2821 attctgcttc gctatcgctt tgcgacacat ccagcatcag gtgattttcc cgataaggtg
     2881 tcattccgtc gtataccacc actccattac ggtttgttgt gcgatatttt tgcccattga
     2941 cataagcatc tttaattcct ggcgcattca tcacagcaaa cgtttcggaa agacggttcg
     3001 ccagattaac gccccccgac caggcgacaa tgccccctga aacactggct ccagcctgtc
     3061 gataagtact cgactgacta taactgccat tcactgtcgc aaccggcgcg ttccaggtca
     3121 aattcgcccc agctgtcgtt tcatttccct gattctgata actcaggttg accccatagt
     3181 taaactgatc ccggcttcca acggttcctg ataatcccgt attatttgag gcaaacccct
     3241 gatcatcaaa cgtcgttgag ttagacatat atatttgccg acgaggcgtc gtaacgtcat
     3301 caccccaatc aaagggaatc gatataaaaa tattaaaacg tttctcttca tgatgattct
     3361 cgtcatacgt ctggcttgcc gcgaggatat agcttatccg ccgccagtta ttggaataac
     3421 tcaactgata atccttactg ctgccgctac gcccccagta atctcgccat aacgtactta
     3481 aggacacaga cccccagcct tctggcaatg actggctcat attggcagag aagctatttt
     3541 tacggccaaa atcgttctgg taataatcgg caatgtcata gacatcgttt tcatcacggc
     3601 gataattatc tttattgttt gcccaaacgt gatcattaaa tgtccggtaa tcacgcgatg
     3661 aataacgcca ggccgccaga ccaaaacgcg tcgacgtttg gctcacaaat ttgttgtagg
     3721 caatttgata actttgcccg tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg
     3781 tggcatcgac ggaaatggca ccaatgcgtg tattccagcc agtccccaaa gtaaacgcgt
     3841 aataattatt cgcgaccatc gagccaccat acagcgtcaa taaattatta aaaccatact
     3901 gataacccgc ctggacaaaa tcactttgtt tgctcgcccc ttcaatatgg ctacgacccg
     3961 ccgcaaaatc atatttcgac acgccgggtt gcagcatatt tggcaccgct gcataaggca
     4021 ccagataggt ggttaccgaa ccgtccgcct ctttcacgct gacatcaaga tctgctccac
     4081 caccggccaa ctgcaaatct gtaatcgcaa acgggccagg aggaacctct ttctgataaa
     4141 ccacaaaacc attctgttca atagttacca gcgcgttact ctgagcaatc ccctgcaccc
     4201 gtggcgtaaa attttgtttc gagttaggca acatctgcat atcacgaaac aaccgcacac
     4261 ctctgaagcg aacagaatca aaaatatcgc ttgatgtgta catatcaccc acgcgaagcg
     4321 tgccgagaag ttgggcaaat ccacgttcca gatacagggt attgcttttc cacacccctg
     4381 gattgttatt tgttttactg aaactggcat cagaatgcag ttgccacccc agtaaattta
     4441 acccgctgtt aaaacgtaca tatgtactct tactattacc cgacgcttta tagtcactgt
     4501 aatactgact cacataataa gaggtataaa acgcattaat accccgctcc cagttttccg
     4561 gtggaacata gccactttcc agttcttcca cccaggcctg cgggacactg aaatcgagac
     4621 gaaaaacccc gatatcccag gtatagctcc caccctgaac aagttgctca aatgttaaac
     4681 attgcttacc gctggcgaag ttatcgctat taatgcctaa ccgcttgata acttctcttg
     4741 ataaacatgt ttcttgcggg ttgtctttaa caataatctc atatttcccg cgccattgct
     4801 tattgacata aatatcaatg tcatactgcc ccggtaaggg ttgattatca tcaagacgaa
     4861 tatttgcaac ctgctggtct ttcattccac ctatcataaa atgggtatca aaggtttctt
     4921 cagctgcata agcttcaatg ccgagcaata acgctacgat tgctgatgca agtggggtca
     4981 ttctcaacat aggcagctcc tgcaattaaa ttttgtcact aatatagttg ccgtggtcat
     5041 cgataatggt cagataccag ttatttgcat tattactttt gacattaaca ctctgacttt
     5101 ctaagggggc aatcattata gtttcataat tgactttaac attattagct ttgacatccg
     5161 aaatcgtcac ccaattagct gagtcatttt ttatcaccaa tccattgcca ctgcgattta
     5221 ccagtaactt tttaaaggtc gctttattta ccggagcaat acccgctggc cggtaaaaca
     5281 acttaattct gttttgcatc gcaaacttca gtgcattttt gccttcttgc tctggactat
     5341 ttggtggaat atccagaaca ttcagataaa aaatgctttc tttattagtg ggcagtttat
     5401 tcggcataat tttgattttt acttgctgcc cggaatttgc ccctattttt gccactggtg
     5461 gcgttaacat gaaaggaacc tgaatttttt ctggtggtaa cgacgtatcg ccatcatcaa
     5521 tccacgcctg cagcagcgaa gaacggtttc cctggttcat caactgcacc ataacttctt
     5581 tattttctgc cgggtaaata atgcgcgtcc cgtagataac aattccggca tgtgcaggaa
     5641 ggaccataga aaaaatgagt aaagataata atcctttcat cttaatgcct cttaaattaa
     5701 aaagccgcca tggggatagc ggccatgttt attacctgga gaaacttaca attttattta
     5761 ttaatcctgc gccacacgca taaccagaga agataacaca tcacctgatt gcactgagtt
     5821 atctgtcagt ttcaggtatt ttgctttaaa cggaatagca tattgacctt ccgctgtttt
     5881 ataactctca atgttaaaag gtgcagaaac atccaggaca tcgctgttat cacctaaata
     5941 gagatagtaa cccacggtat cgttgctact ggctatcgcc ttatcgttac cggttatttt
     6001 atcgttggta atcaacgaga gttttacacc agcaacttct ttcgggcagc taatttttaa
     6061 catgctgctg gttacaccga ctgcatcatc ataggtagaa tatttagctt taccttcaaa
     6121 gacagtggtt ggtacagtcg gcatattaat acttactgaa tcaccacccg cttcgaattt
     6181 acacggagat tctgcaatat tccccttaat agttaatgtt ccggtatcaa cgtctgcagc
     6241 aaatactcca gcgctcataa aaaaataaga aaagacagcg gcagcaataa ttgaacgttt
     6301 cataatatgt tccttaataa aatttatttt tggttgttaa gagtctgtat ctcttggaaa
     6361 caaatttctc acatcaaaaa agttgccgca atgtaaacac attgtttta
//
LOCUS       DAZFVO020000226         6337 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFVO020000226 DAZFVO020000000
VERSION     DAZFVO020000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.228"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..330
                     /locus_tag="ACHKNB_RS23390"
                     /old_locus_tag="pgaptmp_004678"
     CDS             <1..330
                     /locus_tag="ACHKNB_RS23390"
                     /old_locus_tag="pgaptmp_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4198053.1"
                     /translation="DADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRI
                     MLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFL
                     SINKLSQ"
     gene            336..1079
                     /locus_tag="ACHKNB_RS23395"
                     /old_locus_tag="pgaptmp_004679"
     CDS             336..1079
                     /locus_tag="ACHKNB_RS23395"
                     /old_locus_tag="pgaptmp_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4198054.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMAPEDWLRAEMQGEIVALVHSHPGALPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFV"
     gene            1016..1618
                     /locus_tag="ACHKNB_RS23400"
                     /old_locus_tag="pgaptmp_004680"
     CDS             1016..1618
                     /locus_tag="ACHKNB_RS23400"
                     /old_locus_tag="pgaptmp_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4198055.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPSFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1679..5158
                     /locus_tag="ACHKNB_RS23405"
                     /old_locus_tag="pgaptmp_004681"
     CDS             1679..5158
                     /locus_tag="ACHKNB_RS23405"
                     /old_locus_tag="pgaptmp_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198056.1"
                     /translation="MGKGSSKGHTPREAKDNLKTTQLLSVIDAISEGPIEGPVEGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSLTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPRL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEVIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHNYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLI
                     VKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDWHPTASISDLLV
                     VVMKKATAGISIS"
     gene            5226..5825
                     /locus_tag="ACHKNB_RS23410"
                     /old_locus_tag="pgaptmp_004682"
     CDS             5226..5825
                     /locus_tag="ACHKNB_RS23410"
                     /old_locus_tag="pgaptmp_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4198057.1"
                     /translation="MRKVYAAILSTAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSVYAMAGVAYSRVSTFFGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5890..>6337
                     /locus_tag="ACHKNB_RS23415"
                     /old_locus_tag="pgaptmp_004683"
     CDS             5890..>6337
                     /locus_tag="ACHKNB_RS23415"
                     /old_locus_tag="pgaptmp_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4198058.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAA"
BASE COUNT         1438 a         1569 c         1993 g         1337 t
ORIGIN      
        1 gacgccgatc cggagcagga ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg
       61 agcgcggtca gtgcttcctt tgtgctctcc acaccgacgg aaacggatgg tgccgttttt
      121 ccgggccgca tcatgctggc taatacctgc acctggacct atcgcggtga tgagtgcggt
      181 tatcacggtc cggctgtcgc ggatgaatat gaccagccga cgtccgatat cacgaaggat
      241 aaatgcagca aatgcctgag cggttgtaag tttcgcaata acgtcggcaa ctttggcggc
      301 ttcctttcca ttaacaaact ttcgcagtaa atcccatgac agagacagaa tcagcgattc
      361 tggcgcacgc ccggcgatgt gcgccagcgg agtcgtgcgg cttcgtggta agcacgccgg
      421 aaggggaaag atattttccc tgcgtgaata tctccggtga gccggaggcg tatttccgga
      481 tggctccgga ggactggctg cgggcagaaa tgcagggtga gattgtggcg ctggtccaca
      541 gtcaccccgg tgctctgccc tggctgagtg aggccgaccg gcggctgcag gtgcagagtg
      601 atttgccgtg gtggctggtc tgccgggggg cgattcacaa gttccgctgt gtgccacatc
      661 ttaccgggcg gcgctttgag cacggggtga cggactgtta cacgctgttc cgggatgctt
      721 accatctggc gggaattgag atgcctgatt ttcatcgtga ggatgactgg tggcgtcacg
      781 gtcagaatct ctatctggat aatctggagg ccacagggct gtatcaggtg ccgttgtcag
      841 cggcgcagcc gggcgatgtg ctgctgtgct gttttggttc atcggtgccg aatcatgccg
      901 ccatttactg cggcgacggc gagctgttgc accatattcc tgaacaactg agcaaacgag
      961 agaggtacac cgacaaatgg cagcgacgca cacactccct ctggcgtcac cgggcatggc
     1021 acgcatctgc ctttacgggg atttacaacg atttggccgc cgcatcgacc ttcgtgtgaa
     1081 aacgggggcc gaagccatcc gggcgctggc cacgcagctt ccgtcgtttc gccagaaact
     1141 gaatgagggc tggtatcagg tgcgcattgc cgggcgtgat gcaggcgaaa atgaattatc
     1201 agcccgtctt aatgaaccgc tggcaaatgg tgctgtgatc catatcgtgc cgcgtctggc
     1261 aggagccaaa agtggcggtg tgtttcaggc tgtgctgggg gcggctgtta tggcggttgc
     1321 tatatggatg ccgggggtag gaattatggc gagtaatctg ctgttttctc tcggtgccag
     1381 tatggtgctc ggtggtgtgg cgcagatgct ggcaccgaaa gccagaactc cccgcacaca
     1441 gacaacggat aacggcaaac agaacaccta tttttcttca ctggataata tggttgccca
     1501 gggcaatgtc ctgcccgttc tgtacggtga aatgcgtgtg gggtcgcggg tggtatctca
     1561 ggagatcagc acagcagatg aaggggatgg tggtcaggtt gtggtgattg gtcgctgatg
     1621 caaaatgttt tatgtgaaac cgcctgcggg cggttttgtc gtttatggag cgtgaggaat
     1681 gggtaaagga agcagtaagg ggcatactcc gcgcgaagcg aaggacaacc tgaagaccac
     1741 gcagttgctg agtgtgatcg atgccatcag cgaagggccg attgaaggtc cggtggaggg
     1801 attaaaaagc gtgctgctga acagtacgcc ggtgctggac agtgagggga ataccaatat
     1861 ctccggcgtc acggtggtgt tccgggccgg tgagcaggag cagactccgc cggagggatt
     1921 tgaatcctcc ggctccgaga cggtgttggg tacggaagtg aaatacgaca cgccgatcac
     1981 ccgcgccatc acgtcggcaa acatcgaccg actgcgcttt accttcggtg tgcaggcact
     2041 ggtggaaacc acctcaaagg gggaccggaa tccgtcggaa gttcgcctgc tggttcagat
     2101 acagcgtaat ggtggctggg tgacggaaaa agacatcacc attaagggca aaaccacctc
     2161 gcagtatctg gcctcggtgg tggtggataa cctgccgccg cgcccgttta atatccggat
     2221 gcgcagaatg acgccggaca gtaccacaga ccagctgcag aacaaaacgc tctggtcgtc
     2281 atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggcactgg tcggcgtaca
     2341 ggtggattcg gagcagttcg gcagccagca ggtgagccgt aattatcatc tgcgcgggcg
     2401 tattctgcag gtgccgtcga attataaccc gcagacgcgg caatacagcg gtatctggga
     2461 cggaacgttt aaaccggcat acagcaacaa catggcctgg tgtctgtggg atatgctgac
     2521 ccacccgcgc tacggcatgg ggaaacgtct tggtgcggca gatgtggata aatgggcgct
     2581 gtatgtcatc ggccagtact gcgaccagtc ggtgccggac ggctttggca gcacggagcc
     2641 gcgcatcacc tgtaatgcct ggctgaccac acagcgcaag gcgtgggatg tgctcagtga
     2701 tttctgctcg gcgatgcgct gtatgccggt atggaacggg cagacgctga cgttcgtgca
     2761 ggaccgaccg tcggataagg tgtggaccta taaccgcagt aatgtggtga tgccggatga
     2821 tggcgcgccg ttccgctaca gcttcagcgc cctgaaggac cgccataatg ccgttgaggt
     2881 gaactggatt gacccggata acggctggga gacggcgaca gagcttgtgg aggacacgca
     2941 ggccattgcc cgttacggtc gtaatgtcac gaagatggat gcctttggct gtaccagccg
     3001 ggggcaggca caccgagccg ggctgtggct gattaaaacg gagctgctgg aaacgcagac
     3061 cgtggatttc agcgtcggcg cagaagggct tcgccatgta ccgggcgatg ttattgaaat
     3121 ctgtgatgat gactatgccg gtatcagcac cggtggtcgt gtgctggcgg tgaacagcct
     3181 gacccggacg ctgacgcttg accgtgaaat cacgctgcca tcctccggta ccacgctgat
     3241 aagcctggtt gacggaagtg gcaatccggt cagcgtggag gttcagtccg tcaccgacgg
     3301 cgtgaaggtg aaagtgagcc gtgttcctga cggtgttgct gaatacagcg tatgggggct
     3361 gaagctgccg aggctgcgcc agcgactgtt ccgctgcgtg agtatccgtg agaacgacga
     3421 cggcacgtat gccatcaccg ccgtgcagca tgtgccggaa aaagaggtca tcgtggataa
     3481 cggggcgcac tttgacggcg aacagagtgg cacggtgaac ggtgtcacgc cgccagcggt
     3541 gcagcacctg accgcagaag tcactgcaga cagcggggaa taccaggtgc tggcccgctg
     3601 ggacacgccg aaggtggtga agggcgtgag cttcctgctt cgcctgaccg tggcagcgga
     3661 tgacggcagt gagcggctgg tcagcacggc ccggacgacg gaaaccactt accgcttcac
     3721 acaactggct ctggggaact acaggctgac agtccgggcg gtaaatgcgt gggggcagca
     3781 gggcgatccg gcgtcggtat cgttccggat tgccgcaccg gcagcgccgt cgcggattga
     3841 gctgacgccg ggctattttc agataaccgc cacgccgcat cttgcggttt atgatccgac
     3901 ggtacagttt gagttctggt tctcggaaaa gcggattgcg gatatcaggc aggttgaaac
     3961 cacagcacgc tatcttggca cggcgctgta ctggatagcc gccagtatca atatcaggcc
     4021 gggccataat tattattttt acgttcgcag tgtgaacacc gttggcaaat cggcattcgt
     4081 ggaggctgtt ggtcagccga gtgatgacgc atccggctat ctggattttt tcaaaggcga
     4141 gatagggaaa acccatctgg ctcaggagct gtggacgcag attgataacg gtcagcttgc
     4201 gcctgacctg gctgaaatca ggacatccat tacggatgtc agcaatgaaa tcacacagac
     4261 cgtcaataag aaactggaag accagagtgc agcgatccag cagatacaga aggttcaggt
     4321 tgatacaaat aataatctga acagcatgtg ggctgtgaag ctgcagcaga tgcaggacgg
     4381 acgcctttat attgcgggta tcggtgccgg tattgagaac acccccgacg gcatgcagag
     4441 tcaggtactg ctggcggcag acaggattgc gatgattaat cctgcaaatg gcaacacaaa
     4501 accgatgttt gttggtcagg gtgatcagat attcatgaat gaagtgttcc tgaaatatct
     4561 gacggctccc accattacca gcggcggtaa tcctcctgca ttttccctga caccggacgg
     4621 aaagctgacc gctaaaaatg cagatatcag tggcagtgtg aatgcgaacg ccgggacgct
     4681 caacaacgtt acgataaatg aaaactgtca gattaaaggg aaactgtcag ccaaccagat
     4741 tgaaggcgat attgtcaaaa cagtgggtaa ggctttcccg cgggactccc gggcaccgga
     4801 gcggtggcca tcagggacca ttaccgtcag gatttatgac gatcagccgt ttgaccggca
     4861 gattgttatt ccggcggtgg cattcagcgg tgctaaacat gagagagagc atactgatat
     4921 ttactcctca tgccgcctga tagtgaagaa aaatggtgct gaaatttata accgtaccgc
     4981 gctggataat acgctgattt acagtggtgt tattgatatg ccagctggtc acggccacat
     5041 gacgctggag ttttcggtgt cagcatggct ggtaaatgac tggcatccca cagcaagtat
     5101 cagcgatttg ctggttgtgg tgatgaagaa agccaccgca ggcatcagta tcagctgaat
     5161 tttataaccc atatacgggc gccagaaatg gcgccttttt tattgcagaa aagcgagagg
     5221 taattatgcg taaagtttat gcagccattt tgtccacagc catctgtctg gccgtatccg
     5281 gtgcgcctgc atgggcgtct gagcagcagg ccacactgag cgcagggtat cttcatgccc
     5341 gtacgaacgc tcccggcagc gataatctga acgggattaa cgtgaaatac cgttatgagt
     5401 ttacggacac actggggctg attacgtcat tcagttatgc caacgctgaa gatgagcaaa
     5461 aaacgcatta cagcgatacc cgctggcatg aagattccgt gcgtaaccgc tggttcagcg
     5521 taatggcggg gccgtctgtg cgcgtgaatg aatggttcag cgtgtatgcg atggcgggtg
     5581 tggcttacag ccgtgtgtcg actttcttcg gggattatct ccgcgtaact gacaacaagg
     5641 ggaaaacgca cgatgtgctg accggaagtg atgacggtcg ccacagcaac acgtctctgg
     5701 cgtggggggc tggcgtgcag tttaacccga ccgaatccgt ggccattgat attgcttatg
     5761 aaggctccgg cagtggtgac tggcgcactg acggtttcat cgtgggtgtc ggttataaat
     5821 tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg gcttttttgt
     5881 ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg aaaaccggta
     5941 cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt ggtgaacacg
     6001 gtggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacggtcag
     6061 tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg
     6121 tatgaagact cccgacccgg tacgctgaat gattttctcg gtgccatgac ggaggatgat
     6181 gcccggccag aggcactgcg ccgttttgag ctgatggtgg aagaggtggc gcgtaacgcg
     6241 tccgcggtgg cacagaacac ggcagccgcg aagaagtcag ccggcgatgc cggcacatca
     6301 gcccgtgagg cggcaaccca tgcgactgat gctgcag
//
LOCUS       DAZFVO020000227         6292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFVO020000227 DAZFVO020000000
VERSION     DAZFVO020000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.229"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            79..1008
                     /gene="pfkB"
                     /locus_tag="ACHKNB_RS23420"
                     /old_locus_tag="pgaptmp_004684"
     CDS             79..1008
                     /gene="pfkB"
                     /locus_tag="ACHKNB_RS23420"
                     /old_locus_tag="pgaptmp_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HGU4198059.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLRSHDDTQKIYAYLSR"
     gene            1109..1399
                     /gene="ghoS"
                     /locus_tag="ACHKNB_RS23425"
                     /old_locus_tag="pgaptmp_004685"
     CDS             1109..1399
                     /gene="ghoS"
                     /locus_tag="ACHKNB_RS23425"
                     /old_locus_tag="pgaptmp_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HGU4198060.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1505..2365
                     /gene="yniA"
                     /locus_tag="ACHKNB_RS23430"
                     /old_locus_tag="pgaptmp_004686"
     CDS             1505..2365
                     /gene="yniA"
                     /locus_tag="ACHKNB_RS23430"
                     /old_locus_tag="pgaptmp_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HGU4198061.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2406..2942)
                     /gene="yniB"
                     /locus_tag="ACHKNB_RS23435"
                     /old_locus_tag="pgaptmp_004687"
     CDS             complement(2406..2942)
                     /gene="yniB"
                     /locus_tag="ACHKNB_RS23435"
                     /old_locus_tag="pgaptmp_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HGU4198062.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3089..3757
                     /gene="hxpB"
                     /locus_tag="ACHKNB_RS23440"
                     /old_locus_tag="pgaptmp_004688"
     CDS             3089..3757
                     /gene="hxpB"
                     /locus_tag="ACHKNB_RS23440"
                     /old_locus_tag="pgaptmp_004688"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HGU4198063.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3920..4510
                     /gene="ydjM"
                     /locus_tag="ACHKNB_RS23445"
                     /old_locus_tag="pgaptmp_004689"
     CDS             3920..4510
                     /gene="ydjM"
                     /locus_tag="ACHKNB_RS23445"
                     /old_locus_tag="pgaptmp_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HGU4198064.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4643..6034
                     /gene="tcyP"
                     /locus_tag="ACHKNB_RS23450"
                     /old_locus_tag="pgaptmp_004690"
     CDS             4643..6034
                     /gene="tcyP"
                     /locus_tag="ACHKNB_RS23450"
                     /old_locus_tag="pgaptmp_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HGU4198065.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLTVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6038..>6292)
                     /gene="ydjO"
                     /locus_tag="ACHKNB_RS23455"
                     /old_locus_tag="pgaptmp_004691"
     CDS             complement(6038..>6292)
                     /gene="ydjO"
                     /locus_tag="ACHKNB_RS23455"
                     /old_locus_tag="pgaptmp_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HGU4198066.1"
                     /translation="NLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQECI
                     DKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1542 a         1554 c         1571 g         1625 t
ORIGIN      
        1 tctaataaat catattgtta atttcttcac tttcggctga ttcggtgcca gactgaaatc
       61 agcctatagg aggaaatgat ggtacgtatc tatacgttga cacttgcgcc ctctctcgat
      121 agcgcaacaa ttaccccgca aatttatccc gaaggaaaac tgcgctgtac cgcaccggtg
      181 ttcgaacccg ggggcggcgg catcaacgtc gcccgcgcca ttgcccatct tggaggcagt
      241 gccacagcga tcttcccggc gggtggcgcg accggcgaac acctggtttc tctgttggcg
      301 gatgaaaatg tccccgtcgc tactgtagaa gccaaagact ggacccggca gaatttacac
      361 gtacatgtgg aagcaagcgg tgagcagtat cgttttgtta tgccaggcgc ggcattaaat
      421 gaagatgagt ttcgccagct tgaagagcaa gttctggaaa ttgaatccgg ggccatcctg
      481 gtcataagcg gaagcctgcc gccaggtgtg aagctggaaa aattaaccca actgatttcc
      541 gctgcgcaaa accaagggat ccgctgcatc atcgacagtt ctggcgaagc gttaagtgca
      601 gcactggcga ttggtaacat cgagttggtt aagcctaacc aaaaagaact cagtgcgctg
      661 gtgaatcgcg aactcaccca gccagacgat gtccgcaaag ccgcgcagga aatcgttaat
      721 agcggcaagg ccaaacgggt tgtcgtttcc cttggtccac aaggagcgct gggtgttgat
      781 agtgaaaact gcattcaggt ggtgccacca ccggtgaaaa gccagagtac cgttggcgct
      841 ggtgacagca tggtcggcgc gatgacactg aaactggcag aaaatgcctc tcttgaagag
      901 atggtccgtt ttggcgtagc tgcggggagt gcagccacac tcaatcaggg aacacgtctg
      961 cgctcccatg acgatacgca aaaaatttac gcttaccttt cccgctaaca aaaacattcc
     1021 cccagcattg ggggaatcac caccaacctg tcggcaacgc gtttctccga ctatgctcaa
     1081 aagtcatgtg ataacaaagg ggtgaactat ggccagtggc gatcttgtcc gttatgtcat
     1141 aaccgtcatg ttgcatgagg atacattgac tgaaattaac gagttgaata attacctgac
     1201 tcgcgacggt tttttgctca ccatgacgga tgatgaggga aatatccatg agctggggac
     1261 taacactttt ggacttatca gtacccaaag tgaagaagaa attagagaac tggtttcggg
     1321 gcttacccaa agtgcaaccg gcaaagatcc tgaaatcacc atcacgacct gggaggaatg
     1381 gaatagcaac agaaaataaa tggtttttgg gcaataatca gtctgtggtg tgcgttagct
     1441 cgtgttttta caccgcattc ttgcgctaac cttatgatct ggcagacaac atgggagaga
     1501 catcatgtgg caggcaatca gtcgtctttt gagcgagcag ttaggtgaag gcgaaatcga
     1561 actgcgtaat gaactgcctg gcggagaagt ccatgccgca tggcatttgc gctatgcagg
     1621 acatgacttt ttcgtcaaat gtgatgaaag ggaactgctt cccggtttta ccgccgaagc
     1681 cgaccaactg gagttactgt cgcgtagtaa aaccgtcacc gtgcctaagg tttgggcagt
     1741 aggcgctgac cgtgactaca gttttctggt gatggattat ctcccacctc gtccgctgga
     1801 tgcgcatagc gcatttattc ttggtcagca aattgcgcgt ttacatcaat ggagtgacca
     1861 accacaattt ggcctcgatt ttgataactc gctctccaca actccacagc ccaacacctg
     1921 gcaacgtcgc tggtcaacgt tttttgctga acaacggatt ggctggcagt tggaactggc
     1981 agcagagaaa gggatcgctt tcggcaatat cgacgccatc gtcgagcata ttcagcagcg
     2041 tctggcctcg catcaaccgc agccttctct gttgcacggc gatttatggt ccggcaactg
     2101 tgcgctgggt ccggatggcc cgtacatttt cgaccctgcc tgctactggg gtgaccgaga
     2161 gtgcgacctg gcgatgttac cgctgcatac tgaacagccg ccacaaatct atgacggcta
     2221 tcagtcagta tccccgctac ctgccgattt ccttgaacgt caaccggttt accaactcta
     2281 cacgctgtta aatcgtgcaa gattatttgg cggtcagcat ttggttattg ctcagcagtc
     2341 attggataga ttattagcag catgatatgg gttgaggata atggccgctc cgtgcggcct
     2401 tttgattaaa taaacccaag cagagaaaag aacacataac ctgcagcaat acaaataacc
     2461 ggcaggatat atagtgagaa aaactgcagg aaaatagtat ggtgcggaac aacaatacgg
     2521 gactcaattt gttcacgcgt tagcccctct tcccctttgg ctttttccag aatgagttga
     2581 tcttcaacac cttcccgcaa gaagcgcgcc tggcgactca tccgggcacc ggaatcctgc
     2641 aacgccagtc cgacaaaaat caggatgaaa atcacccaga acataacgtt cacgccacca
     2701 ttaaaattgg gcgtcgggga gttataccag aacagattca aaaaaggcgt attcgcctgc
     2761 atcatatcga tcatgacatg agtaaagtcg agcatgaccg cattaatgcc ttcctggttt
     2821 tcctgccggg tattcataaa tttcagcaaa gaaatcaatg tagagatcaa tgcaggtata
     2881 aaaatcaccc aacccaaaat ccttttcaaa acagcaatgc gtccagcttg ttgatacgtc
     2941 atgagttctc cttgattaag acgcgtcgtt tcacttagtt tacctgtaga tatctgtttt
     3001 cgcccattct ttaaaggcga tatgataggc gcttaatcat aagcacggct taatacctta
     3061 cacataatgc tctaaaggag aggttgtaat gtcaaccccg cgtcagattc ttgctgcaat
     3121 ttttgatatg gatggattac ttatcgactc agaaccttta tgggatcgag ccgaactgga
     3181 tgtgatggca agcctggggg tggatatctc ccgtcgtaac gagctgccgg acaccttagg
     3241 tttacgcatc gatatggtgg tcgatctttg gtacgcccgg caaccgtgga atgggccaag
     3301 ccgtcaggaa gtagtagaac gggttattgc ccgtgccatt tcactggttg aagagacacg
     3361 tccattatta ccaggcgtgc gcgaagccgt tgcgttatgc aaagaacaag gtttattggt
     3421 gggactggcc tccgcgtcac cactacatat gctggaaaaa gtgttgacca tgtttgactt
     3481 acgcgacagt ttcgatgccc tcgcctcggc tgaaaaactg ccttacagca agccgcatcc
     3541 gcaagtatat ctcgactgcg cagcaaaact gggcgttgac ccactgacct gcgtagcgct
     3601 ggaagattca gtaaatggca tgatcgcctc taaagcagcc cgcatgcgtt ccattgtcgt
     3661 tcctgcgcca gaagcgcaaa atgatccacg ttttgtatta gcaaacgtca aactctcatc
     3721 gctgacagaa ctcaccgcaa aagaccttct cggttaatga ccaggggcag tgatctcgct
     3781 gcccctggtt ctttatctga atttgcattc aactgacgga ttaatcgtca atttaagaga
     3841 aagagttaca ccgtcaccac ttccgtgcac tgtataaaaa tcctatactg tacgtatcga
     3901 cagtttagtg agttttatca tgacggcgga aggtcacctt ctcttttcta ttgcttgtgc
     3961 ggtatttgcc aaaaatgccg agctgacgcc cgtgctggca cagggtgact ggtggcatat
     4021 tgtcccttcc gcaatcctga cgtgtttgtt accggacatc gatcacccaa agtcgtttct
     4081 tgggcagcga ttaaaatgga tatcaaaacc gatcgcccgc gcttttgggc atcgtggttt
     4141 tacccacagt ctgctggcgg tatttgcgct gctggcaacc ttttacctta aggttccgga
     4201 aggctggttc attccggctg atgcgctaca aggaatggtg ctgggttatt tgagccacat
     4261 acttgccgat atgctgacac ccgccggtgt tcccctgctc tggccatgcc gctggcgttt
     4321 ccgcttgcct atcctggttc cccaaaaggg caaccaactg gaacgtttta tctgcatggc
     4381 attatttgtc tggtcggtat ggatgcccca ttcattaccc gagaacagcg ctgttcgttg
     4441 gtcatcgcaa atgatcaata ccttgcagat ccagtttcat cggcttatta agcatcaggt
     4501 tgaatactaa aaagacaaaa atcacctttc tggaataagc aattccattt gaatataaga
     4561 gccagctcac agttctgtta atcttgcgcc aacactatga ctgctacgca gtgatagaaa
     4621 taataagatc aggagaacgg ggatgaactt tccattaatt gcgaacatcg tggtgttcgt
     4681 tgtactgctg tttgcgctgg ctcagacccg ccataaacag tggagtctgg cgaaaaaagt
     4741 gctggtgggt ctggtgatgg gtgtggtttt tggccttgcc ctgcatacca tttatggttc
     4801 tgacagccag gtacttaaag attctgtaca gtggtttaac atcgttggta acggctatgt
     4861 tcaactgctg caaatgatcg ttatgccgtt agtcttcgcc tctattctga gcgcggttgc
     4921 ccgtctgcat aacgcatctc agttaggcaa aatcagtttt ctgaccatcg gtacgctttt
     4981 gtttaccacg ctgattgcgg cgctggtcgg tgtgctggtc accaacctgt ttggtttgac
     5041 ggctgaaggt ctggttcagg gtggtgcaga aactgcacgt ctgaacgcca ttgaaagtaa
     5101 ctatgttggt aaagtctctg atctgagcgt tccgcagctg gtcttgtcct ttatcccgaa
     5161 aaacccgttt gccgatctta ccggagccaa tccgacgtca attatcagcg tggtaatttt
     5221 tgccgcattc ctcggcgtag ctgcattgaa actgctgaag gatgatgcgc cgaaaggtga
     5281 acgcgtctta gccgctatcg ataccctaca aagctgggtg atgaaactgg ttcgccttgt
     5341 catgcaattg accccttacg gcgttctggc tctaatgacc aaagtggttg caggttctaa
     5401 cttgcaagac atcatcaaac tgggaagttt cgttgtcgcg tcctacctcg gtctgctgat
     5461 tatgtttgca gtgcatggca ttctgctggg cattaatggc gtgagtccgc tgaagtactt
     5521 ccgtaaggta tggcctgtgc tgacgtttgc ctttaccagc cgttccagtg ctgcgtctat
     5581 cccactgaat gtggaagcac aaacgcgtcg tctgggcgtt cctgaatcca tcgccagttt
     5641 cgccgcctct ttcggtgcaa ccattggtca gaacggctgc gccggtttgt atccggcaat
     5701 gctgacggtg atggttgcgc ctacggttgg cattaacccg ctggacccga tgtggattgc
     5761 gacgctggtc ggtattgtta ccgttagttc cgcaggcgtt gccggtgtcg gtggtggtgc
     5821 aactttcgcc gcactgattg tgctgcctgc gatgggcctg ccagtaaccc tggtggcgct
     5881 gttaatctcc gttgaaccgc ttatcgacat gggccgtacg gcgctaaacg ttagtggctc
     5941 gatgacagct ggcacgctga ccagccagtg gctgaagcaa accgataaag ccattctgga
     6001 tagcgaagac gacgccgaac tggcacaccg ttaattctta tgctggcaac ggtccgtttt
     6061 gtataggggc cgttgcttta ctttttaaat gttccatcgc ggcgatgcat actgactcat
     6121 atttaaacag atcgtcagcg aaaaaaatgt ccaacttatc aatacattcc tggctcaatg
     6181 aatgtgcctg tagtgagaag cttaaaacct catacccgag acgttttcta agtgaactat
     6241 ctggaatact cataatatct tcaagcacac cacttcccat tcccggaaga tt
//
LOCUS       DAZFVO020000228         6284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFVO020000228 DAZFVO020000000
VERSION     DAZFVO020000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.230"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..448)
                     /locus_tag="ACHKNB_RS23460"
                     /old_locus_tag="pgaptmp_004692"
     CDS             complement(<1..448)
                     /locus_tag="ACHKNB_RS23460"
                     /old_locus_tag="pgaptmp_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4198067.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAA"
     gene            complement(513..1112)
                     /locus_tag="ACHKNB_RS23465"
                     /old_locus_tag="pgaptmp_004693"
     CDS             complement(513..1112)
                     /locus_tag="ACHKNB_RS23465"
                     /old_locus_tag="pgaptmp_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4198068.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(1179..4577)
                     /locus_tag="ACHKNB_RS23470"
                     /old_locus_tag="pgaptmp_004694"
     CDS             complement(1179..4577)
                     /locus_tag="ACHKNB_RS23470"
                     /old_locus_tag="pgaptmp_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198069.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDNEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRMLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     AIQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSELLVSTARTAETTYRFRQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(4638..5210)
                     /locus_tag="ACHKNB_RS23475"
                     /old_locus_tag="pgaptmp_004695"
     CDS             complement(4638..5210)
                     /locus_tag="ACHKNB_RS23475"
                     /old_locus_tag="pgaptmp_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4198070.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLTAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPTATSTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(5207..5950)
                     /locus_tag="ACHKNB_RS23480"
                     /old_locus_tag="pgaptmp_004696"
     CDS             complement(5207..5950)
                     /locus_tag="ACHKNB_RS23480"
                     /old_locus_tag="pgaptmp_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4198071.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRSWRASAFTGICNDLAAASTFV"
     gene            complement(5955..>6284)
                     /locus_tag="ACHKNB_RS23485"
                     /old_locus_tag="pgaptmp_004697"
     CDS             complement(5955..>6284)
                     /locus_tag="ACHKNB_RS23485"
                     /old_locus_tag="pgaptmp_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4198072.1"
                     /translation="DADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRI
                     MLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFL
                     SINKLSQ"
BASE COUNT         1313 a         1953 c         1583 g         1435 t
ORIGIN      
        1 ccgcagcatc agccgcatgg gttgccgcct cacgggctga tgtgctggca tcactggctg
       61 acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgcc acctcttcca
      121 ccatcagttc aaaacggcgc agtgcctccg gacgggcatc atcctccgtc atggcaccga
      181 gaaaatcatt cagcgtaccg ggttgagaat cttcatacac ggtgatggtc ccggcatgtg
      241 acggcgggaa tccttccacc aacagaataa cgctgtactg accgtactca acgtccatgc
      301 tgtaacgccc ggcttcatcc ggattttctg aggccagcgt gttcaccacc accgtggtgc
      361 tgttacgttt tgctttcagc tggattgtgc agttctgtac cggttttcct gtgccgtctt
      421 tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg cctgaaccgg
      481 cgggctgtca taacactgtg ttacctggct aatcagaact tataaccgac acccacgatg
      541 aaaccgtcag tgcgccagtc gccactgccg gaaccttcat aagcaatatc aatggccacg
      601 gattcggtcg ggttaaactg cacgccagct ccccacgcca gagacgtgtt gctgtggcga
      661 ccgtcatcac ttccggtcag cacatcgtgc gttttcccct tgttgtcagt tacgcgaaga
      721 taatccccgg agaaagtcga cacacggctg taagccacac ccgccatcgc atacgcgctg
      781 aaccattcat tcacgcgcac agacggcccc gccatcacgc tgaaccagcg gttacgcacg
      841 gaatcttcat gccagcgggt atcgctgtaa tgggtcagct ggcgattctt gtctcctgca
      901 tagctgaatg acgtcaccat ccccagtgtg tccgtaaact cataacggta tttcacgtta
      961 atcccgttca gatcatcgct gccgggaacg ttcgtcgagg catgaagata ccccgcgctc
     1021 agcgtggact gatgttcaga cgcccatgca ggcgcaccgg atacggccag acaaatggct
     1081 gcggacaaaa tggcggcata aagtttacgc ataattacct ctcgcttttc tgcaataaaa
     1141 aaggcgtcat ttctgacgcc cgttatgggt tataaaattc agctgatact gatacctgct
     1201 gtggatttct tcatcaccac aaccagcaga tcgctgatac tggttgttgg tgtccagtta
     1261 ttcgctcctg atgaagatac ggtgaatgtc agtgtcagtg tcccctgtcc ggcaggcata
     1321 tctataactg aggaaaatac gccctgagca tccgtcgtgg actgattaaa aatctcctga
     1381 ccattgcggg tcactcttaa ccggcaggtt gaataccagt atgactgttg gttattactg
     1441 ttgaaattct catgcttacc accgcggaat aacactggcg gtatcatgac ctgccggtca
     1501 aatttctgat catcactgat tcttaccgtg atggtgccac tggcataact gttcgtgcgg
     1561 gggaaagact tgctgaccgt tttgacaata tcaccttcaa tctggttggc tgacagtttc
     1621 cccttaatct gacagttctc attaatcgtg acgttgttga gcgtccctga gttcgcattc
     1681 acactgccac tgatatccgc atttttagcg gtcagctttc cgtctgatgt cagggaaaat
     1741 accggaggac tgccaccgct ggtaatggtg ggtgccgtca ggcgtttcag gaacacgtcg
     1801 ttcatgaata tctggttgcc ctgcgccaca aacatcggcg tttcattccc gtttgccggg
     1861 tcaataaacg cgatacggtt agcggcaacc agaaactggc tcagcttgcc ttcctccgtg
     1921 tcctccatac tgaggccaat acccgcgaca taatgtttgc cgtctttggt ctgctcaatt
     1981 ttgacagccc acatggcatt ccatttatcg ttggcatcct tccactcttt cgaaaactca
     2041 tccagtctgc tggcgttatc ctccgtcaga tctacttttt ccagcagctc tttaccgaga
     2101 tgggattcgg ttatcttgcc tttgaaaaaa tccaggtaac cttccgcatc atcgctcgcc
     2161 cgaccgacgg cctccacgaa tgccgatttg ccaacggtgt tcacactgcg gatataaaag
     2221 taataatcat ggcccggttt gatattgata ctggcggcta tccagtacag ccccgtgcca
     2281 aggtagcggg ctgtggtttc aacctgcctg atatcggtaa tccgcgtttc cgagaaccag
     2341 aactcaaact gtaccgtcgg atcataaacc gcaagatgcg gcgtggcagt tatctgaaaa
     2401 tagcccggcg tcagctcaat ctgtgacggc gctgccggtg cggcaatccg gaacgatacc
     2461 gatgccggat cgccctgctg tccccacgca tttaccgccc ggactgtcag cctgtagttc
     2521 cccagcgcca gttgcctgaa gcggtatgtg gtttccgccg tccgggccgt gctgaccagc
     2581 agctcactgc cgtcatccgc tgccacggtc aggcgaagca ggaaactcac gcccttcacc
     2641 accttcggcg tgtcccagcg cgccagcacc tgatattccc cgctgtctgc ggtgacttcg
     2701 gcggtcaggt gctgcaccgc tggcggcgtg acaccattca ccgtgccgct ctggtcgccg
     2761 tcaaagtgcg ccccgttatc cacgatggcc tctttttccg gtacatgctg cacggcggtg
     2821 atggcatacg tgccgtcgtc gttctcacgg atactcacgc agcggaacag gcgctggcgc
     2881 agcgtcggca gcttcagccc ccacacgctg tattcagcaa cgccgtcagg aacacggctc
     2941 actttcacct tcacgccgtc ggtgacggac tgaacctcca cgctgaccgg attgccactt
     3001 ccgtcaacca ggcttatcag cgtggtaccg gaggatggca gagtgatttc acggtcgagc
     3061 gtcagcgtcc gggtctggct gtttaccgcc agcacgcgcc cgccgatgct gataccggca
     3121 tagtcatcat cacagatttc aatgacatcg cccggcacat ggcgaagccc ttctgcgccc
     3181 acgctgaagt ccacggtctg cgtttccagc agttctgttt taatcagcca cagcccggcg
     3241 cggtgtgcct gcccccggct ggtacagcca aaagcatcca tcttcgtgac gttacgaccg
     3301 taacgggcaa tggcctgcgt atcctccaca agctctgttg ccgtctccca gccgttgttc
     3361 gggtcaatcc agttcacctc aacggcatta tggcggtctt tcagggcgct gaagctgtag
     3421 cggaacggcg cgccatcatc cggcatcacc acattactgc ggttataggt ccacacctta
     3481 tccgacggtc ggtcctgcac gaacgtcagc gtctgcccgt tccataccgg catacagcgc
     3541 atcgccgagc agaaatcact gagcacatcc cacgccttgc gctgtgtggt caggtacgca
     3601 ttacaggtga tgcgcggctc cgtgccacca aagccgtccg gcactgactg gtcgcagtac
     3661 tggccgatga catacagcgc ccatttgtcc acatccgccg caccaagacg tttccccatg
     3721 ccgtagcgcg ggtgggtcag catatcccac agacaccagg ccatgttgtt gctgtatgct
     3781 ggttttaacg ttccgtccca gataccactg tattgtcgcg tctgcgggtt atagttcgac
     3841 ggcacctgca gaatgcgccc gcgaagatga taattacgac tcacctgctg gctgccgaac
     3901 tgctccgagt ccacctgtat ggcgaccagt gcagtgttcg ggtagcactg tttcacatcg
     3961 atgatttcgg tgtatgacga ccagagcgtt ttgttctgca gctggtctgt agtactgtcc
     4021 ggtgtcatcc tgcgcatccg gatattgaac gggcgcggag gcaggttatc caccaccacc
     4081 gaggccagat actgcgaggt ggttttgccc ttaatggtga tgtctttttc cgtcacccag
     4141 ccaccgttac gctgtatctg aaccagcatg cggacttccg acggattcct gtctcccttt
     4201 gaggtggttt ccaccagtgc ctgtacaccg aaggtaaagc gcagacggtc gatgtttgcc
     4261 gacgtgatgg tccgggtgat cggcgtgtca tatttcactt ccgtacccag cacagtctcg
     4321 gagccggagg attcaaaccc ctccggcggt gactgctcct gctcacctgc ccggaacacc
     4381 accgtgacgc cggagatgtt ggtattcccc tcattgtcca gcaccggcgt actgttcagc
     4441 agcacgcttt ttaatccatc caccggacct tcaaccggcc cttcactgat ggcatcgatc
     4501 acactcagca gttgcgtgga cttcaggttg tccttcgctt cgcgcggggt atgcccctta
     4561 ctgctgcctt tacccattcc tcacgctcca taaacgacaa aacctcccgc aggcggtttc
     4621 acataaaaca ttttgcatca gcgaccaatc accacaacct gaccaccatc cccttcgtct
     4681 gccgtgctga tctcctgaga aaccacgcgt gaccccacgc gcatttcacc gtacagaaca
     4741 ggcagaacat tgccctgggc aaccatgtta tccagtgatg aaaaataggt gttctgctta
     4801 ccgttatccg tgctggtcgc tgtgggcgtc cgggctttcg gtgccagcat ctgcgccaca
     4861 ccaccgagca ccatactggc accgagagaa aacaggatgc cggtcatacc accggcccca
     4921 atggctgccc cccatgctgt aagggtggct ccggcggtaa agaatgatcc ggcaatggcg
     4981 gctgctccca ggacaatctg gaatacacca cctgacttgg ccccggcgac tctgggaaca
     5041 atatgaatca cagcgccatc aggcagagtc tcatgtaact gcgccgttaa tccggacgtg
     5101 ctgacatcct gcccggcaat ccgtacctga taccagccgt cgctcagttt ctgacgaaac
     5161 gccgggagct gtgtggccag tgcgcggatg gcttcagccc ccgttttcac acgaaggtcg
     5221 atgcggcggc caaatcgttg caaatccccg taaaggcaga tgcgcgccat gaccggtgac
     5281 gccagaggga gtgtgtgcgt cgctgccatt tgtcggtata cctctctcgt ttgctcagtt
     5341 gttcaggaat atggtgcagc agctcgccat caccacagta aatggcggca tgattcggca
     5401 ccgatgaacc aaaacagcac agcagcacat cgcccggttg tgctgatgac aacggcacct
     5461 gatacagccc tgtggcctcc agattatcca gatagagatt ctgaccgtga cgccaccagt
     5521 catccccgcg atgaaaatcc ggcatctcaa tccccgccag atgataagca tcccggaaca
     5581 gcgtgtaaca gtccgtcacc ccgtgctcaa agcgccgccc ggtgagatgc ggcacacagc
     5641 ggaacttgtg aatcgccccc cggcagacca gccaccacgg caaatcactc tgcacctgca
     5701 gccgccggtc agcctcactc agccagggca gaccaccggg atgactgtgg accagtgcca
     5761 caatctcacc ctgcatctct gcctgcagcc agtcctccgg agccatccgg aaataatcct
     5821 ccggctcacc ggaaatattc acgcagggaa aatatctttc cccctccggc gctctcacca
     5881 cgaagccgca cgactccgct ggcgcacatc gccgggcgtg cgccagaatc gctgattctg
     5941 tctctgtcat gggattactg cgaaagtttg ttaatggaaa ggaagccgcc aaagttgccg
     6001 acgttattgc gaaacttaca gccgctcagg catttgctgc atttatcctt cgtgatatcg
     6061 gacgtcggct ggtcatattc atccgcaact gccggaccgt gataaccgca ctcatcaccg
     6121 cgataggtcc aggtgcaggt gttggccagc atgatacgtc ccggaaaaac agcgccatcc
     6181 gtttccgtcg gcgtggacag tacaaaggag gcactcaccg cgctcagttc gctgcactgc
     6241 tcgatgcgcc agcggctgat cacctcctgc tccggatcgg cgtc
//
LOCUS       DAZFVO020000229         6255 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFVO020000229 DAZFVO020000000
VERSION     DAZFVO020000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.231"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3593)
                     /locus_tag="ACHKNB_RS23490"
                     /old_locus_tag="pgaptmp_004698"
     CDS             complement(<1..3593)
                     /locus_tag="ACHKNB_RS23490"
                     /old_locus_tag="pgaptmp_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4198073.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDSALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLRFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTDDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADSAVWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEIILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDS"
     gene            complement(3613..4074)
                     /locus_tag="ACHKNB_RS23495"
                     /old_locus_tag="pgaptmp_004699"
     CDS             complement(3613..4074)
                     /locus_tag="ACHKNB_RS23495"
                     /old_locus_tag="pgaptmp_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HGU4198074.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(4102..5987)
                     /gene="tssI"
                     /locus_tag="ACHKNB_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /pseudo
     CDS             complement(4102..5987)
                     /gene="tssI"
                     /locus_tag="ACHKNB_RS23500"
                     /old_locus_tag="pgaptmp_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
BASE COUNT         1165 a         1875 c         1735 g         1480 t
ORIGIN      
        1 caactgtcat agccatagga ctcgtcgcct tcgccatagc ccagcccggt gcctgaggct
       61 gtcaccgccg tgacctggcc gttccggtcc agccggtact cccgccgtgt ttcccgtccg
      121 taatcttccc ggctggtgac agccgacaca ttacccgcgc tgtcgtaacg gtactcccgt
      181 accagtgagg ggagcagccc gccccatgtg gcttctgccg ggaaaagtcc ggcgcgctgg
      241 ctgacaggct gaccggctgc attccagccc tgttcacagg caaaaccccg gttactgtgc
      301 cggagcactt cacgtcccgc ctcatcacgg ctgaacgtca gcgtctcatc ccccggcagg
      361 ataatctccg tcagttctcc ggcggcgttg tagcggtacg tggttttcag cagggattca
      421 tcggggtgtc tgtcgcgttc atcttccggt ggcaggatac agcggatgac ggtgtgttcg
      481 tcggggtagt ggtattcaat ggcccgtcgg ccctgagtgg tgacattcac ccgcccgcgt
      541 tcgtcgtagc cggtgcgctg tgatgtgccg tccgggctga agatacccgt cagcctgccg
      601 gatttgtcat aggcatactg cgttttgcct tccggggtga tgacttctgc caggcggcct
      661 gcggcgtccc ggctgtagag gatggtttcc tccaggccgt tgaccctggc ggtacacagg
      721 ccatcggcgt catagcggta acgccagacc acgccgcccc agtcacgctc ttccgtcacc
      781 cgtccacagt tgtccagaat ataacggtat gactcgccct gcggattgat gacttctgtc
      841 agttgcagcg tgtctttatc gtagcggtac tgccagcggt ggccttcacc gtccgttcgc
      901 gccaccggca aatcgaacgg gccgtattcc atcgtccaga caacgccgtt gccgtccgtc
      961 caggccagca ggttgccgtg gcggtcatag cgaaatcctt ccgcaccggt tccagcacgt
     1021 tcaatgccgc tcagtttatg gtgctgctgc cactgtaacc gcactgactg accgccgggg
     1081 ccgcgcatct tcaccggcag gtcgtgacca ctgtattcgg tgtggtgttc acggcccatc
     1141 acgtcggtga gtgtttccgg ccggttgtgg tggtcgtact ctatgcccag acgccgttca
     1201 tcctggcgga atatccccga cagcagcccc tgcggcgtgt acgccagccg ggtctcttcg
     1261 ccctgtggcg acaccaggct taccggcagg ccgcgttcat cacactgctg tgtccacgtc
     1321 tgcccggctg ggtcggtgat gttgctgagc aggccgcgct catcatagcg gtactgccac
     1381 accgcgctgt ctgccgccgt ataacggctt aactgaccgt gtgcggcgtt gtattcaaac
     1441 ttctccaccc gcccggcagg ggagtggcgg gccagcaggt gatggaattc atcccattca
     1501 tacgtggtgg tgctgccgtc cggcgctgct tcccgcagaa tattgccgtc aggatcatag
     1561 taataacggt gagtgccctt gccgtccgtc agggtggtgc agccgtcacc atagtccagc
     1621 gtggcgttgt aatagccctc cgccccggtg acggtcacgc accggccctg ttcatcgtag
     1681 gtgaaacggc tccacgtctg gtcgttatcg gaccagcgga tgatccggtc cgcagcgtcg
     1741 tactcataaa acaggtggta gtccagccgc gcatccgctt cggtcagccg cccccgcgca
     1801 tcctgttcgt aggtcgccag cgtctgtatg ccgttatccg tgcggtgaat ggctttcagg
     1861 tagccgctgg catggtacgt cagtttaagc cggatcccgt cggtgtgact gacacccgtg
     1921 atccggtact ggctgtcgcg ctcaaacgtg atgcggttgc tggcggtgtc gctcatcccg
     1981 cccagcatgt acatccggtc tgcggggctg tgtgccagac ggtcatcgtc ggtatcgtca
     2041 gtcaccgggt ggcgcaggcg cattcccgga aaggcgtcat aaaagaagcg gctgctcagg
     2101 gcatccctgt cgaacagctc caggtaatca tcgcggcggc gcagaatgta ctgggcgtga
     2161 tacgggttaa ccgccgtatc actgtcaccg tcgaaggcaa atctcagcgt ggctccctgg
     2221 ctgatgatat ccacccggtt tccctgttca cgcacccagg cgtattcgct ccaggagtcg
     2281 ctccagccca cgcccgtcag gccatgcagt ggcagtacag aactgtggaa acgggtgaag
     2341 gagaggggga gggtctggcc aagggtgaag tcggtacgtt cgtcgcagta tgcgccggtt
     2401 accggatcga caggatctgt tacccatctg atgactttat tggcaatact ttttaacgac
     2461 ttacctgttt tccccagcaa cgcgccgagc tttgcgccca gcagcttccc gcttttcccc
     2521 agcaggtttc ccagtttgcc gagtctcgcc agattcttca gttttcccag cagtcccgct
     2581 ggcggcagaa gatagctggc tatcgttatt cctcccagga tcattctctg ccatggcggg
     2641 aactccggct ggatatccag ataggtgacc tgctcacccc caataaagac gtccggcgaa
     2701 ccttccacga ttgccgcgct gcactccagt ttgtcgccct tgcgcgcggc aggctgattg
     2761 ttgataaaga cgttgctgct gccctgggct atcctcggtg gtttgttctc cttgtcgcag
     2821 agcgccgagt cctcaccggc ttccgggaaa tccacctctt ttttgatttc gccacgcccg
     2881 cccaccatgg tggcaaactc cattaatacc ggaccggcgg gaaacagaag cgatgcgccg
     2941 gcgctgacgc gatccataaa accggcattg ctgtccgtca gggtgataac agcgttcccc
     3001 caggccttcg ccagtggtac tgctttttcc gctgccgatt caagcaggtc agtggcaata
     3061 tcaccccatg agggttcccc ctgtgggact tcaggtattg ctccaggtgg gggaggaggg
     3121 ggaagggtga cggcggccct ggcagcgggc tttccgttaa tgtttacatt ttcagaaccg
     3181 gtattgatgg cgccgtccgt gtcacaaaag ctgtttatcc atgactcaag ctggttctgt
     3241 ttctcctcca cataatcact cgccttatag ctggcataca tcagcgcagg caccagaacc
     3301 agcgcataag ggccggtggc gcaggcggcc ataatcaggc aaccttctgc gatggtcact
     3361 atcgccccca gcacggcccc tgccagtgcc cccagaaaac ttttatgctg aatttcatcg
     3421 cccagcctgg cggcaggtgg gccggacttc tttgctgcca gcatctgtgc aatggctttg
     3481 tcgttggctg cccgtttcat ctggctggcg accatcgcct gctgtgacag cattcgcatt
     3541 gccagcgtac cgcctgcggt cgctccctgt ggcccgcctg gtccttcact catgtgttat
     3601 ccctcgttta tgtcagcgcg gctggtcctt ttggatgtca ttctccggtg gcaacggggt
     3661 gaagctcttt atcacctcca gcagcatcgt cttttgactg ttcttcaggg ataaggaggt
     3721 gctgaaagtg aaggtcagca gcgtatctcc acgccgctgg aatacggcta tctggctaat
     3781 ccgcccctga ttcccctcat cttccggacc gttccaggaa taatccagca gccaggccgg
     3841 ggcctctgcc agagtgattt cccggcaggc atgttcggtg tacccatcaa gtttgccctg
     3901 gtacagcgcc agcgtttcct ggtacagctc tggtgtcagg gttccctgtg gaacggcttc
     3961 gcgggtaatg accaggttga tgccactgtt atctgtggac accaggacct gcattgactg
     4021 atcctgccat tgagacggca gttgaatgtg gccttcatta aaagtgaact gcataaaata
     4081 ctccctgata atctgagtga atcagttgat gaaaacagaa ccgcccacga tggtgatgcc
     4141 atccttcgac agcctgatta ccgtcgattc cccgacctgt agctgaatgc catccggcgt
     4201 cagcgcaatt ttgctttcct ccacagccag caaaatttcc ttaatcgccg tataagcctg
     4261 tataccgtcg ccttcgccac cattgtttac agcgtcaaca tgcaccgaac cggcaatcgt
     4321 gcctctgtcc tgaaagacat cctcagataa agaccctttc agtactccgg tgatcttgtg
     4381 gcctttttcc accgtaagaa ggcggttacc ttcctggatc gtgaccgtct ggtcattttt
     4441 tacggtttcg cggtgatcgt gttttacggt ggtggtacgg tcgttgagca cttcggtatc
     4501 catgttcttc tgcgcgtgga tgtagacctg ttctctccct gtcgcatcat caaacttcag
     4561 ctcattaaat ccgctgccca tgtacgtttt ggagcggatg gtcatctgcg tctttgttcc
     4621 cggcaggctg ccgggggtgc ggttttcctg gtggtaggtg cgccccataa tgatcggctg
     4681 gtccggatcg ccgttgagga agtctacaat cacctcctga cccacacgcg gtatcgccag
     4741 gtggccaaaa ccggtgcctg cccacgcctg tgccacacgg atccagcacg aactgtcttg
     4801 gtctgccggg ttataacggt cccagttgaa cttcacccgc acgagaagat ttcctcgcct
     4861 gccggtcctg tcacgacagc gctctgcggg ccgtccacca gcggtttcag caacggctgt
     4921 ggtcgccatg ttctgtctgc cgggatcacc gcaaagtgat tattcagcgt ggtgcctgaa
     4981 ccactgcgtc ccggcaccgc ctgtggctgt tcgccgtgca gttcacttgc caccacctgc
     5041 cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg tcccggccat
     5101 atctccggcg agcggctcat tccccgcgcg gtttctgcgt tgtttcgcca gccgtccatc
     5161 tgccagcggg caaagttctg cccgtgggcg ctcttgaaac gtccggggta gtcatacact
     5221 tcatactgcg tgcgctggta gtcctggtgc tggccttcct gttcaaaacg tccggcccag
     5281 ccggggcgtt taaaggtgta gtctttggtc accacggaag aagggcggat ttgtgcgctg
     5341 tagcggaact ggctgatgca gagggtgctc acctcggtac gggtgttcgg gttccatggg
     5401 atttcaaaag attcgggcag atggcggact gtgtcgcaca gcaccaggct ctggtcggta
     5461 cttttgtaag catgctcctc ataaaagaag atgccttcct ccgccgccat ccggcacagg
     5521 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
     5581 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
     5641 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
     5701 tgcaccttca tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc
     5761 accacgcctt tcacccggcg ctgtacttca tcgccctgcc atatcgtcag gtaggccatt
     5821 ttgtccagca cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg
     5881 agggaaaaga gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcatcc
     5941 ggcggcaggc cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt
     6001 aaagtgaata atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca
     6061 gacgttatta gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt
     6121 actttacaga aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt
     6181 tcttggcgtt attcaggaca ggtacgcata tcacaacgag taacaaaaac gtaaagttaa
     6241 accaatattc ataat
//
LOCUS       DAZFVO020000230         6245 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFVO020000230 DAZFVO020000000
VERSION     DAZFVO020000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.232"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>6245
                     /locus_tag="ACHKNB_RS23505"
                     /old_locus_tag="pgaptmp_004701"
     CDS             <1..>6245
                     /locus_tag="ACHKNB_RS23505"
                     /old_locus_tag="pgaptmp_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HGU4198075.1"
                     /translation="APTAEELVRSQMAEGDAGPTAQELTERPRLPAPGDIHPGQGYPL
                     PGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGRQGKILEGETVRRGL
                     PSPDAQNVTAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPELAQQAEVRRQAGGNR
                     DIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGAAKHRYAQLAETLGK
                     TLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDIPAKTKHNTAGWGES
                     IKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLPPSQMDRASAYRVVS
                     GVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLTKAKVWFAEQEEKSQAKG
                     DEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDARKYIDSHRDELV
                     RHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFRGVQFGNYVEGPRRQ
                     ADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAINLTKG
                     NGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDAEYPV
                     RQEVYDAFKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYVQDKARTA
                     GVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETDKGVAFYS
                     RKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVVKSQKEAE
                     ALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAMEHVVTPAE
                     YQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQPNKFVAAW
                     ERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYTPREDGAGASSQYSR
                     SGKPDPFKVPEGEGERYRDDLARMMKSLRSTDLTVNIGRTPPVLRHLGAPDLPLVISR
                     DTVRKATNGVKHVVPMDVIERLPELMHDPDAIYHSATERNAVVMLLDAVDKNGDPVVS
                     AVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKLSRDNLLHRGLQLPK
                     GEHSYRGSADKILYPEDIRKGPHYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENI
                     RKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLY
                     ARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLA
                     RRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQA
                     MGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDKDYWQVFTNERPDTM
                     RAIAERVVPETGETRHEVVERPVPMAMMADRYFTTKKNGKTYYIKLHDPRLMRAMKNM
                     GPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLK
                     GLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEFVEDGGKTGWFNMGD
                     LEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENALRLSAYKHARDAGL
                     SRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLVRTLGHLNGEGPLLE
                     RLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMK
                     SVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGVLGAFSPIG
                     SETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRST
                     PEAYKAFASWLNAFSGGSQYRPGAVDITPESLKYWVDYISGGTGRFISKTTDAAVKSL
                     NGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDEN
                     NGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNR
                     EYNKKVGV"
BASE COUNT         1585 a         1467 c         1909 g         1284 t
ORIGIN      
        1 gcgccgacgg cggaggagct tgtacgttca cagatggctg aaggtgatgc cggtccgaca
       61 gcacaggagt taactgagcg tccacgcctg cccgctcccg gcgatattca tcccggacag
      121 ggatatccgt taccgggaga ggtggcgcgt acgccggatg aaaatcaggc cggacgtggt
      181 ggtcgtttta ccacaaccgg tgaggttaag ggccagagtt tccagaaagg acaagctccg
      241 gcaccggaaa acgccgctgg tcgccagggg aaaatactgg agggcgaaac agttcgtcgt
      301 ggtctgccgt caccggatgc gcagaacgtg acagctccgg tacgtgaagg gttaccggct
      361 cctgacattg cgcgtaatgt tcgtatgcct cagcctgaat cacttccccg cactgtacgg
      421 gactcactgc ctgagctcgc acagcaggca gaagtacgcc gacaggccgg aggaaatcgt
      481 gacatcccgc agcctgagac aatcgcacct gaatctgaaa caactgtctc tactgacagg
      541 gaagctaccg tgcgcggagg tgaagtcagg ggcaaaaaaa ttgaagactt tggcgaggaa
      601 attaaagggg cagccaaaca ccgttatgca cagcttgccg aaacactggg taaaacgctg
      661 gaagacaggg attatgccac gcagccgctg agcaaactgt tcccgaaacc ggactacgca
      721 aaactggcga acgaaggtgc tgatgctgat accctggcaa tgatagcgct gtatcgtagc
      781 gatattccgg cgaagacgaa acacaatacg gcaggctggg gggagagcat aaaaaaagta
      841 cgacacagtg tatcggaaat gctgaacgga acggtcagcg cgaaacgcct cgcagaatgg
      901 atggaaggta gaatgccctc ccgttacgcg gatacctggc aactgttacg cactctgcca
      961 ccctcacaga tggacagggc ttctgcttat cgggtggtat cgggtgtgta tcaggcggca
     1021 ggagggaagc gttacgatcc gccacagaaa ctttattcac tgcgcaataa ggacaataag
     1081 gggagtaacc tctttttctc ggaaagcagg gatgaattac tgacaaaggc gaaagtctgg
     1141 tttgcagagc aggaggaaaa atcacaggcg aaaggtgatg aaaaaacagc accgtcaccg
     1201 gatgacaaaa tccgctttga cgtttaccgg aatacccgca gtggagatat ttttatcgct
     1261 tacggtaaaa acaaaatgcg ggtgagaggt ggctttaagt cagccagtga tgcgcgtaag
     1321 tacattgatt cacatcgtga tgagcttgtt cgtcatgtga aggagatgcg ggagatttcg
     1381 cgtgaggagc agcgcaacgc caccaaccgc gaccgtaccg gaccagaacg ccgcaagggg
     1441 aatgtttcac cggagcagtt cagtgatgcg tttggtttcc gtggtgtgca gtttggtaac
     1501 tacgtggaag gtccgcgtcg tcaggctgat ttgaaccggg cttatgactc gctgcatgac
     1561 cttgcggaag tactgaatgt accgacaaaa gcgctttccc tgaacggtcg tcttggcctg
     1621 gcatttggtg cccgtggtaa gggtaaggcg gcggcacact atgagccggg tgaggtggca
     1681 atcaacctga caaaaggtaa cggaccgggg gcgctggccc acgaatggtt ccattcactg
     1741 gataattatt ttggtcgtta tgatgtttcc actgacggga agatcacgtc aggtggcgac
     1801 tatatgacgg aagcacaacg tgccgggcgc gtatttaaag acggcagata tgttgatgcg
     1861 gaatatccgg tacgtcagga ggtttacgac gcgtttaaag gcgtgatgaa agcgatcaac
     1921 agcagtgaca tgccgcgtcg ttcagcgctt ctcgatgagg tgcgctcaaa accgtactgg
     1981 tcaacggatg ttgaaatggc ggcgcgtgcc tttgagcgtt atgttcagga taaagcgcgt
     2041 acggctggcg tggagaatga ttatctggtc aatatccgta aggcacctga gcacaacaca
     2101 gataacacct gggcttatcc gacgaatgcg gaactggatg gcggtattcg tgatgcattc
     2161 gatcacctgt tcagtacgct gaaaacccgt gagacggaca agggcgttgc gttttattcc
     2221 cgtaagggcg tgacccgcac acctgaaggc aatctcattt cggatgttaa ccgcagtgcg
     2281 gaagccaaag gcagcccggt cccgcaggtt gaagcggttg cccgtggcgt gatgagtggc
     2341 attaaggaca gtgacctgaa ggtccgcgtg gtgaagtcac agaaagaggc tgaagcgctg
     2401 gcgggtgaat cgttcgacgg ttacggcagg gtgcatgcgt tctatcgtcc ggataaacgt
     2461 gaaattgtcc tggtggcgga taacattcct gacgggcgga ccgttcgcga gaagctgcgt
     2521 cacgagatta tccaccatgc aatggagcat gtagtcacgc cagcggaata tcagacgatt
     2581 attaagaccg tgctgaaaac ccgtgacagt gataacgcca ccatccgtga agcctggcgt
     2641 aaggttgatg cgtcttatgg taaggaatca ccggaagtgc aggcgggtga atttctggca
     2701 catatggcgg agaaacagcc gaataaattt gtggcggcgt gggagcgtgt tgttgccctg
     2761 gtcaaagggg tactgcgtcg cacggggtta ctgaagccga cagagctgaa cgatatcaga
     2821 cttgttcgcg aaactattcg cacgttaggc cagcgtgtgc gggaaggtta cacgccgcgt
     2881 gaggatggcg ctggcgcatc gtctcagtac tcccgtagcg gtaagcctga tccgttcaaa
     2941 gtgccggaag gtgagggcga gcgttatcgt gatgaccttg ccagaatgat gaagtctctg
     3001 cgctcaacgg atttaacggt aaacatcggg cgtacgccgc cggtattgcg tcaccttggc
     3061 gcaccagatt tgccgctggt tatctcccgc gacactgtgc gtaaggccac caatggtgtg
     3121 aaacatgtgg tgccgatgga tgttatcgag agactaccgg aactgatgca cgatccggat
     3181 gcaatttacc actcagcgac agaaagaaat gcggttgtga tgctgcttga tgccgtggat
     3241 aaaaatggtg atccggtggt gtcagcggta cacatgaagg ctgtccggtc gcgtctggaa
     3301 atcaacaagg tagcttctgt ttacggtaca gaaaatggaa aaaaactgaa gagtatggaa
     3361 atgaccgggt taacgttgta ccggagagaa aaattaagcc gcgataacct tctgcacaga
     3421 gggctccaat tgcccaaagg ggaacattct tatcgcggct ctgcggataa aatactctat
     3481 cctgaagata ttcgcaaggg gccgcattac tcccgtacca gcagtctgac accggaagag
     3541 acaattgcat cgcgttttgt gcgccagatg caggataaat tccaggtgct gaaagctgtt
     3601 caggagaata tccgtaaaac tggcggaaaa atagacgaca gtaacaacgc ttatatggcg
     3661 gaagaactct tccacgggaa ggcggaaaac gacctgaacg tgatgaagga gcgctatgtt
     3721 cagccgctgg ctaaattact ggcggactac aaaattgcgc aggccgatct ggatgagtac
     3781 ctctacgccc gtcacgcgcc ggaacgtaac gcgcatatcg cgaaaatcaa cccgaaaatg
     3841 ccggacggcg gttcggggat gaccaacgcg gaagcggcgg aaatcatgca gcgtgtgcgt
     3901 aacagtggca aacaggcaca gtatgaccgt ctggcaggga ttgttgacga tatgctggcc
     3961 cgtcgccgtg agcttatccg tgaggccgga ctggaagaga gcggtgtggt ggatgcctgg
     4021 cagaacgcct accgttacta cgttcctctg aaaggccagg atgttgacgg tgtggtgtca
     4081 ctgccccgta caggtaaagg cttcactatc ggcggacgtg aaagcaggca ggccatgggg
     4141 cgtgcatccc gcgcacagtc tccgtccact caggcgatac aggacttgag cgaatcgctg
     4201 atccgcaatc gcaaaaatga agtaggtaac gccttcctga aactggtaca ggataatcca
     4261 gacaaggatt actggcaggt attcaccaat gagcgaccgg acaccatgcg tgcgattgca
     4321 gagcgtgttg ttccggaaac cggtgaaacc cgtcacgaag ttgtcgaacg tcctgtaccg
     4381 atggcaatga tggcagaccg gtacttcacc accaaaaaga acggcaaaac gtactacatc
     4441 aaactccatg atccgcgcct gatgcgtgcg atgaagaata tgggaccgga aaccagtaac
     4501 gccgtaatcc gcacgctggg gaaagttaac cgcttcctgg caacggtgaa cacgtcgtat
     4561 aacccggaat tcctggtcag taacttcatc cgtgacgtgc agacggcggt gatgaacctg
     4621 aaggcggagc agggaaggag cgacggtaaa ctgaaagggc tggataactt atccgccctg
     4681 gctgtggtga aagacagccg gtctgccatg tctgccgtat acgccagtct gcgtggtaaa
     4741 accctcacgg gcaacggtgc gcagtggcag aaggtgtgga aagagtttgt tgaggatgga
     4801 ggtaaaaccg gctggtttaa catgggtgac cttgaaggcc agcagaagga aatggatcgc
     4861 cttgtatcgc tggcgaaggg gggatggaaa ggccagagta tcggtgcatg gaattcgttc
     4921 cttaaccttg tcgaggatgc caacggggcg gttgaaaacg ctctgcgtct ttctgcctat
     4981 aaacacgccc gtgatgccgg tttgtcacgc cagcaggcgg cgtctcttgc caaaaacatg
     5041 acggtgaact ttaaccgtcg tggtgagcag ggggcgctga tgaactcgct gtacatgttc
     5101 gccaacgcca gcattcaggg aacggcaaat ctggtgagaa cgctcggaca tcttaatggt
     5161 gagggacctt tactggagcg ccttcgctgg aagaatctga atgttccgca gaaaatcgcg
     5221 cttgccgctg tgggagcagg ttatctgctt ggctcgctta accgcagcgt ggcgggtgag
     5281 gatgatgacg gggttaactg gtatgacaag gtgccgtctc atgtgaaaga gcgtaacctc
     5341 gtcattatga aatcggtgtt cgggggcaag gccggagagt actggagtat tcctctgcct
     5401 tacgggtaca acgttttctt cctgctcggg catactgctg aaggtgtggc ggcgggtgac
     5461 ctgacggcgt cccgtgctgc cggtaatgtt gttggtggtg tgcttggtgc attcagcccg
     5521 attggcagtg agacgtcgga aacactgtcc ggggcattgc tgaaaaatgc agcgccgacc
     5581 attctgcgtc cgtttgcgaa ccttgccatg aatgaaaact tcatgggggc gcagatttac
     5641 caggagaaca tgccgtttgg tacaccaaaa cctgacagcc agctgggaag acgttcaacg
     5701 ccagaagcgt acaaggcgtt tgcatcctgg ctgaatgcgt tctctggtgg cagccagtac
     5761 cgtcccggcg cggtggatat cacaccggaa tcgctgaaat actgggtgga ctatatctcc
     5821 ggagggacag ggcgcttcat ttccaaaacc acggatgcgg cggtgaaatc gctgaatggt
     5881 attgatatac cggagcagca ggtgcccttc ctggggaaaa tttcaggtga ggtgatgccg
     5941 tatgctgacc agcagaagat gtacgaccgg atgacagagg ttgcgcagta tcacgcagag
     6001 ctgaagagtc tgactggtgc agaaagaacg gcgttcattg acgagaacaa cggaaaattg
     6061 tcgatgaacg ggcttatgca ggatacccgg aagagactga aggatttgcg taaacagcgt
     6121 gatgccattt acgccgacag tactctcagt ctggcgcaac agtcggcgat ggtgaaatcg
     6181 gtagagcggg atatgaaaat tgccgtggat cggtttaacc gcgagtacaa caaaaaagtg
     6241 ggagt
//
LOCUS       DAZFVO020000231         6188 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFVO020000231 DAZFVO020000000
VERSION     DAZFVO020000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.233"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1671
                     /locus_tag="ACHKNB_RS23510"
                     /old_locus_tag="pgaptmp_004702"
     CDS             <1..1671
                     /locus_tag="ACHKNB_RS23510"
                     /old_locus_tag="pgaptmp_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198076.1"
                     /translation="TTSTGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLN
                     MLLRRSLVEVSIPGELLTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAAS
                     AKSASDSAKTATSRAAEAGQKASEAAEAATRAGESEKAAAADAKNAKQHAETARVAQQ
                     AAGDILERAEAATVSAEEARRMAENARGPQGDQGPKGDTGPRGEKGEPGQRGPQGPKG
                     ERGERGPQGSVGPAGPRGEKGEQGERGPQGLQGLKGETGERGAQGAQGPAGPTGATGP
                     AGPRGETGPQGAQGPAGATGPVGPQGERGIQGPAGPAGPRGETGARGEKGDPGDPGGP
                     PGPKGDTGPAGPAGEKGEPGERGPQGLQGPPGPAGERGPAGEPGAKGETGKPGPAGPQ
                     GQPGPQGLKGDRGEPGPTGPQGPAGERGPQGPRGVAGPVGPAGAAGAKGDAGPAGPAG
                     PRGPAGERGPAGPKGERGEPGPMGPPGPAGDAGNSTVKGIRLGNEMVYRPYSGYSNLS
                     LRSMDGCVLTGIAGTNMPDKSRIDEIYYRPLQMTFGDGSWRSVGIGEV"
     gene            1943..2608
                     /locus_tag="ACHKNB_RS23515"
                     /old_locus_tag="pgaptmp_004703"
     CDS             1943..2608
                     /locus_tag="ACHKNB_RS23515"
                     /old_locus_tag="pgaptmp_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4198077.1"
                     /translation="MIIKSFKLYTPEKSDVPGAMYLKSVDGRDWYESQSLFAADTLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNEMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     DWK"
     gene            3101..4234
                     /locus_tag="ACHKNB_RS23520"
                     /old_locus_tag="pgaptmp_004704"
     CDS             3101..4234
                     /locus_tag="ACHKNB_RS23520"
                     /old_locus_tag="pgaptmp_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4198078.1"
                     /translation="MSKKFSKTILSSAVAGLMLVSSGAMAAEPEVINVGDGTQYQVKL
                     YKNGEVAEVYDVKAGRQFLGAINTTTGSVSTVNNAEIKSAMELFLKENPSAVGQRQAV
                     AQIIGEMHRYSINVPSFKLDNIDTLTVTDIESIKANVDAVSKVITSKTAADYNQAVSN
                     GMSSEAALTVAKDGGAVLKEFNRIDSDIDQLNKDTTFAMDADGNITLDETAGTGERYG
                     VKDVVAEVVEDTTVRVAEDGSLTTARNTQTTKRVNEAVVDLDKSVRTNSQDIATNRKA
                     IQSNSRQLQEHNARLNDHQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALG
                     GYSDKQAVAVGVGYRFNEQTAAKAGIAASDGDVSYNVGVNFEF"
     gene            4323..4796
                     /locus_tag="ACHKNB_RS23525"
                     /old_locus_tag="pgaptmp_004705"
     CDS             4323..4796
                     /locus_tag="ACHKNB_RS23525"
                     /old_locus_tag="pgaptmp_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198079.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYSVILAREFFMPENRKHTPENN
                     ESWVIICDALLGRLMRCMFQGRRYLQLDAEEMTGRLDTYRKIRNGDWVQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYLGLGKNGLRYRSVKHLVGLA"
     gene            4840..4965
                     /locus_tag="ACHKNB_RS23530"
                     /old_locus_tag="pgaptmp_004706"
     CDS             4840..4965
                     /locus_tag="ACHKNB_RS23530"
                     /old_locus_tag="pgaptmp_004706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198080.1"
                     /translation="MKQANLKYVKKRYTAGAMCFMLFVWLGAVVTLCAVAAVMMQ"
     gene            5146..>6188
                     /locus_tag="ACHKNB_RS23535"
                     /old_locus_tag="pgaptmp_004707"
     CDS             5146..>6188
                     /locus_tag="ACHKNB_RS23535"
                     /old_locus_tag="pgaptmp_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198081.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPDHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPVAQDNYGRIYYTDGKFPKVTAA
                     EIATKGEGNFPAASYRLGIPAPTTAPVCTVQRGEAISDDNPNDDETRFYTETFVSAYG
                     EEGPPGPESLEVTVGIPDTPVQLTLSPVPLQDANINRRRIYRSVSGGGEADFLLVAEL
                     EASVLSYTDNIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYA"
BASE COUNT         1666 a         1344 c         1854 g         1324 t
ORIGIN      
        1 accacgtcaa caggctatta cggaatgacg atggatccgg gggagtatgc ggtttcggtg
       61 acggtgaaag ggaagacagc tgtctacgga cgtgtgcgta ttgaggggac cgaaagtacg
      121 gtgacgctca atatgctgtt acgccgcagt cttgttgagg tgagcatacc cggagagctg
      181 ctgacagatt tccggcagat acagaataat gtggctgatg accttgccac tattcgtcgt
      241 ctgaatgaag acacggcgac aaaaaacact caggccacac agtcaaaaga aagtgcagca
      301 gccagtgcga agagtgcatc tgacagtgca aagacggcaa ccagcagggc ggctgaagcc
      361 ggacaaaaag ccagtgaagc tgcagaagca gcgacccggg caggcgagtc tgaaaaggca
      421 gcggcagcag acgcgaaaaa tgcaaaacaa catgctgaaa cagcccgggt ggctcagcag
      481 gcagccggag acattcttga gcgtgcggag gctgcaacgg tcagtgcaga ggaagcccgg
      541 cgcatggctg agaatgcgcg gggacctcag ggcgaccagg ggcccaaagg tgataccggc
      601 ccccggggtg aaaaaggtga gccgggtcag agaggtcctc agggtccgaa aggagagcgc
      661 ggtgagagag gtcctcaggg tagtgtcggc ccggcaggtc cgcggggtga gaaaggcgag
      721 cagggggagc gtggaccaca gggattacag gggctgaaag gtgaaaccgg agaacgtgga
      781 gcacagggcg cacagggacc ggcaggcccg acgggggcaa cggggcctgc tggtcctcgt
      841 ggtgagacgg ggccacaggg tgcacagggg ccagccggag caacaggacc tgttgggcca
      901 caaggagagc gcggtattca ggggcctgcc ggtcctgcgg gcccgcgagg tgaaaccgga
      961 gccagaggtg aaaaaggaga ccccggggac cccggcggcc caccgggacc aaagggtgat
     1021 accggacctg ctggccctgc cggtgaaaaa ggtgaacccg gtgagcgagg gccacaggga
     1081 ttacagggac caccgggtcc tgccggagag agaggtccgg caggcgaacc gggagcgaaa
     1141 ggtgaaaccg gaaaacccgg acctgctggt cctcaggggc agcccggtcc acagggactt
     1201 aaaggtgaca ggggcgagcc aggacctaca ggcccacagg gaccggcggg agagcgggga
     1261 cctcaggggc ctcggggggt agctggtcct gtcggcccgg caggtgcagc gggcgctaaa
     1321 ggtgatgcag gacctgcagg gcctgccggt ccccgaggac ccgccggtga acggggccct
     1381 gccgggccga aaggtgagcg tggtgaaccg ggaccgatgg ggcctccggg gccagcaggc
     1441 gacgcaggaa acagcacagt aaaaggcatt cgtctgggaa atgagatggt gtaccgtcca
     1501 tatagtggct atagcaactt gagcctacgc tcaatggatg gctgtgtgct tacgggaatt
     1561 gcgggaacaa acatgccaga caagagccgt atagatgaaa tctactacag acccttgcaa
     1621 atgacttttg gtgatggaag ctggcgctca gtcggtatag gggaagtgtg agctgttgtt
     1681 cttttcagcg caggtaactg gcagctctgc atacttgtta tattgcgcat aaaaaaccgg
     1741 ggagcagggc gttactcccc ggtaaacata cagaccagac aatgatacca cactgtcagt
     1801 tattattatc atgacataag tcagcgcagg gaatattagt gcatgcacat gtctggtttc
     1861 tttgatctca caacgctttt tttgtgaaaa gaaaaaagat gaggcactga aatcacagtt
     1921 ttatttaaat gagtggacgt tgatgattat taagagtttt aagctataca cccctgaaaa
     1981 atctgacgtg ccgggtgcga tgtacctgaa atcagtagac ggcagggact ggtacgaaag
     2041 ccagtcatta ttcgcggcag acacgctgaa gctggtttat gacagtaacg gtataatcac
     2101 cagcataagt aaggatgtct ctatgctgtg gccggtaaac cagagtgtgg cagaggttgc
     2161 tgatacggaa gaaaaccgca aagctgacat ctcggggcgc tggggttttg acggggagaa
     2221 aatcacggac ctgctgaccg cggagaaagc gcgcgggatg aagggcgatg aaattaacgc
     2281 ctggcgtaat gagatggaag cggcgaacta cacgtttgag cacaatgggc gtaaatggga
     2341 ctacgggaag tcaacgcaga cgcgtcttga gccgtcggtg gcggcagcga aagcggggaa
     2401 actgccggag gcgtttttct ggacggatgc ggaaaacaat gatgtgccgg tgacagcaga
     2461 agagcttata gcactgagtg aagcggcaga gcaggcgatg ttcaccaaag ggatggaaat
     2521 ccacgtccgg cagcgcacca tgaagaagga gctggaaaag ctgaccagtg cggatgagat
     2581 actggcatac agggtggact ggaaataaaa agaaggggag cggatgcacc cctgatatct
     2641 gagtagcatg ctgtttatag ttatcattca ttaaaagaat attaccgtat ggcacggcag
     2701 cagaacatga aatttgtctc agaactgcaa atcagcatta cgggtagtca ggcagataac
     2761 acaacgggat aaaaaaacag caaaatgcat taataaaaaa tttttacagg ttggttgccg
     2821 ggcgttatgt gacgcatatc aaaaacagaa ctgtaacggg agataacaaa atgatggatt
     2881 ctggcgggga gtaacagcgc aatgcgttcc gggatgctgt ttctgagaat gtggctgaaa
     2941 acgcccatgc tatagtaaga aaaatcctaa tagttttata ttaattttta cggaactatt
     3001 cggaataccc ttatgaaagg caacgaagtg tcagtatttt taatgtattc tgacacgcgt
     3061 tgctctgttt gtatacaaat catttcatag ggtataccag atgagcaaaa aattctctaa
     3121 gacaattctt tcttctgctg ttgccggact gatgctggtg agttctgggg ctatggctgc
     3181 ggagcctgaa gttattaatg tcggtgatgg aacgcaatat caagttaagt tgtataagaa
     3241 tggtgaggtt gctgaagtat atgatgttaa agcaggtaga cagtttttgg gagcgataaa
     3301 tacaactaca ggaagtgtaa gcacagtaaa taatgcggaa atcaaatctg cgatggagct
     3361 ttttctgaag gaaaatccat cggcagtggg acagaggcaa gctgttgcac aaataattgg
     3421 agaaatgcat cggtatagca ttaatgtccc ttctttcaag ttagataaca ttgatactct
     3481 taccgtaact gatattgaaa gcatcaaagc aaacgttgat gcagtttcca aagttattac
     3541 ctccaaaacc gcagccgatt ataaccaggc tgtcagcaat ggtatgagtt ccgaagctgc
     3601 actgactgta gctaaagatg gtggtgcagt cttaaaagaa ttcaaccgaa ttgattcgga
     3661 tatcgaccaa ctgaataaag acaccacttt tgcaatggac gctgacggca acattactct
     3721 ggatgagaca gccggtacag gtgagcgcta tggcgtgaag gatgttgtgg ctgaagttgt
     3781 tgaggatacc accgttcgtg tggcagaaga cggttccctg accacagccc gtaacacgca
     3841 gacaaccaaa cgcgtaaatg aagcggtggt tgaccttgat aaatctgtaa gaactaacag
     3901 ccaggacatt gcaaccaaca gaaaagcgat tcagtcaaac agccgccagc tgcaggagca
     3961 caatgcacgt ctgaatgacc atcaacgcca gattcgtgaa aaccacgaag agatgaaacg
     4021 cgcagcggca cagagcgcag cacttgccgg tctgttccag ccgtacagtg tgggcaaatt
     4081 caacgccacg gcagccctgg gtggttacag tgacaaacag gccgttgctg tcggtgtggg
     4141 ctaccgtttc aatgagcaga ccgcagcgaa agcgggtatt gcagcaagcg acggtgacgt
     4201 ttcttacaac gtgggcgtta acttcgagtt ttaattgctg acagtgacat gaagcatgac
     4261 ggcaggtgcg ggattttggc ctgccgtttt tttcatgtgt tcagagacag tgaggagatg
     4321 cgatgaaggt gattctggcg acccgcaacc gttatctgga gtacggcctg caacagatgc
     4381 ttgaggggta cagtgtcata ctggcccggg aattttttat gccggagaac aggaagcaca
     4441 caccggagaa taatgaatca tgggtgatta tctgtgatgc gctgcttggc aggctgatgc
     4501 gctgtatgtt tcagggacgc cgttatcttc agctggatgc ggaggagatg acggggcgtc
     4561 tggatacata caggaaaatc cgtaacggtg actgggtgca gaacacgtat gcgcgtcctc
     4621 tgacgatgtc cgagatggtg gtgatgtttg gttatgtgta ccgtgagtca aagccgtgtc
     4681 atcttgcgcg tgaaatgggg ataaatacga agacggtgaa cacctttctg tatctggggc
     4741 ttggtaaaaa tggcctgagg tacaggagtg tgaagcacct ggtgggactg gcatagccgt
     4801 attgttatcc ggtactgaag agcaatcagg taaattgtca tgaaacaggc aaatctgaaa
     4861 tacgtaaaga agcgctacac agcaggagcg atgtgtttca tgctttttgt gtggctgggt
     4921 gcagtggtga cgctctgtgc agtggctgcc gtaatgatgc aataaagaaa ggggagcaac
     4981 atgctccccg accagaagaa agacgtttga taacaattag tgagttgtta cgtcttgccc
     5041 agaatatcat agcaacactc tgttgtagtg attccgatcg cgattttagc gaattccacc
     5101 ataaatcccc tgatttttaa tcctgaagca gtcaaaggaa tttctatgcc ttatatcgat
     5161 atcaccacga tgcgtgggat gatgccgcgc gttgtgacat ccatgctgcc cgatcattcc
     5221 gctgtactgg cggaggactg ccatttccgg tttggtgtta ttacaccaga acgtcagata
     5281 tccggggttg agaaaacatt cacaattaag ccaaaaacaa tttttcatta ccgtgatgat
     5341 ttttggtttg cgtggccgga tgtggtggat gtgatccgca gtcccgttgc tcaggataat
     5401 tacgggcgga tttactacac tgacggaaaa tttccaaaag tcacggctgc tgaaattgcc
     5461 actaaaggag agggtaattt ccctgctgca tcatatcgtc tggggatccc tgcaccgaca
     5521 acagcacctg tttgtacagt tcagcgcggg gaagcgattt ctgatgataa cccgaatgat
     5581 gatgaaacac gcttttatac cgaaaccttt gtatcggcgt atggtgagga agggcctccc
     5641 ggacctgaat cgctggaagt caccgtgggg atccctgata ctccggttca gttaacactc
     5701 tctccggttc cgttgcagga cgcaaacatc aatcgtcgtc gtatttatcg ttctgtctca
     5761 gggggcggag aagccgattt tttactcgtg gctgagcttg aagcatccgt gctcagttac
     5821 acggacaaca taccggcgaa aaacctcgga ccttctcttg caacatggga ttacctgccg
     5881 ccgccggaga atatgacagg tctttgcctg atggcgaatg gtattgccgc cggatttgcc
     5941 ggtaatgagg taatgttttc agaagcgtat ctgccgtatg catggccgga agtgaatcgt
     6001 catacgacgg cagaagatat tgtggccatc tgtccgctgg gaacgtcact ggtggtggcg
     6061 acaaaggggg agccttatct gttcagtggt gtatcgcctt ccacaatttc tggctccaga
     6121 attccttcca tgcaggcatg cctgagccga agaagtatgg tggcgatgga gggattcgta
     6181 ctctatgc
//
LOCUS       DAZFVO020000232         6112 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFVO020000232 DAZFVO020000000
VERSION     DAZFVO020000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.234"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(66..551)
                     /locus_tag="ACHKNB_RS23540"
                     /old_locus_tag="pgaptmp_004708"
     CDS             complement(66..551)
                     /locus_tag="ACHKNB_RS23540"
                     /old_locus_tag="pgaptmp_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4198082.1"
                     /translation="MTTVSHNFTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(641..1459)
                     /locus_tag="ACHKNB_RS23545"
                     /old_locus_tag="pgaptmp_004709"
     CDS             complement(641..1459)
                     /locus_tag="ACHKNB_RS23545"
                     /old_locus_tag="pgaptmp_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4198083.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFAEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHTLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLGCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPALQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLKRALWVMAETMLESMR"
     gene            complement(1550..1783)
                     /locus_tag="ACHKNB_RS23550"
                     /old_locus_tag="pgaptmp_004710"
     CDS             complement(1550..1783)
                     /locus_tag="ACHKNB_RS23550"
                     /old_locus_tag="pgaptmp_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4198084.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            complement(1854..4700)
                     /locus_tag="ACHKNB_RS23555"
                     /old_locus_tag="pgaptmp_004711"
     CDS             complement(1854..4700)
                     /locus_tag="ACHKNB_RS23555"
                     /old_locus_tag="pgaptmp_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HGU4198085.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNDGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDILESHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTTAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSDSSAEGYNGQATLNVTF"
     gene            4894..>6112
                     /locus_tag="ACHKNB_RS23560"
                     /old_locus_tag="pgaptmp_004712"
     CDS             join(4894..5209,5209..>6112)
                     /locus_tag="ACHKNB_RS23560"
                     /old_locus_tag="pgaptmp_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4198086.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEQLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCL"
BASE COUNT         1330 a         1718 c         1680 g         1384 t
ORIGIN      
        1 ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gctgctgcat tgtccctgcc
       61 tccattcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc
      121 cgcagccggt aataatggac cgtcatggca taacactccg tacgacaggc gtgatggctg
      181 tacgccatca gacaggcggc aataccggca gcttccgggc tcatttcagc gcggttaccg
      241 ttcatggcat tgaacagtac ccatgtttct ccatcatcat catccggttc gggggccata
      301 aatgccccgc cgttgttcag ggtgtacaga ttccagatac catcgcagta gttttcgcaa
      361 agacggtcca tccagccgaa gatacggggc tccagggtga cccactgtgg aatgaggcca
      421 aagtgctgcg gccagaagct gatgcgctgt tcatcaggga ctatggtggc aaccaactga
      481 gactggttat tccctgatgc agtggttacg gaaacagaag gtgtggtgaa attatgcgag
      541 acggttgtca tgagattatt ccttataaaa agtaaatgaa tggaagaagt cgcgggccgg
      601 gacggacatg agtcagaact gcgctttcag ggaaaaggca tcagcgcatg ctctccagca
      661 tcgtttctgc catcacccac aacgcccgtt tgagcttaat gtcggtatcg atgctgtgaa
      721 tggcccgggt atggatacgt tttcctctgg cactgcgacc ggaaatcccg cctttcagca
      781 tattttcctg gatggtctga taagcactcc acaggtcctt accgtaatcc tcccggcgtc
      841 gtggcgtcag aatgtcggca gtggtgacgg gctgatgttc atcaccataa cggtaagtca
      901 gcgccgcctg tgccagcgcc tggagtgccg gtggcggcag gaccagcgac tgcatggcat
      961 cacgcttttc ctcaatccgg tcaaacacgc ccaccacttc gtaagcacct tctatgaccc
     1021 tgtccaccac gtttccccgg tgtggcaccc ggacttctcc cagcgactga ccgcagccca
     1081 ggccattggt acaaatggca cgaaaatatc cgggtaacat ctggtagctg gatgaaccgt
     1141 catgggagtt gagcagaata atttcaggca catgctgacc ggttatctgt ccggcccgcc
     1201 gcagacgcag cgtgtgtttg gtatattccc ggcggctctg gtcgcgcaca cgggtctggc
     1261 aggcgaagaa cggctgaaag ccttcccgct gcagattttc caggacggtg atggtgggaa
     1321 tgtacgcata ccgttcactg cgggaggtgt gccggtcttc cgcaaaaata ctcgggacgt
     1381 ggtacatcag ttcttcgcgt gtcagcgggc gctcccggcg gatctgattt acataaccaa
     1441 aacggctggc taatcgcata attggctcct tatgaatgat taagcggtat aaatgaaaaa
     1501 gccgcgtctt tcggagaaga cgcggcctga tggatcaagt gaatgacgtt tattgcctga
     1561 gaattccctc actggcgaga tacggattta gctgttttcc ggcatcaggt tcaaaattcc
     1621 agcaccgcca gcgcaactgg ccttcagcgt tgcggatcac cagccggaaa tgcgttccct
     1681 ggtcatcttc aataagcaca ttaaggtaag ctgttgtgac ggcaagagcc tggttgcgtg
     1741 agaaagggcc ttcgggcaat aaacgtgatg tacaacccgg catatctgtt gctccctgta
     1801 agtaaaaggc cccggtcatg atgaccgggg cctgaagggg agtgacctga ttatcagaaa
     1861 gtcacattca gcgtggcctg accgttataa ccttcagcgc tgctgtcgct gacgctgtgg
     1921 gcataaccgg cctgaacgcc cagggtgata ttttcccgga cacgggcttc cagtccggcc
     1981 tgcaggtcca gtgacgtgcc attctgggac ggtgagaacg tcatgttact gccggctgcg
     2041 gttgtaccca tgctcatgtc accccgggag ctgaaggtgc ggataacaga aggctgtacc
     2101 caccagttca ccggcaattc actcacgctg tgttttgcac tgtcgcgcag ggtgtcacgg
     2161 gatgaggtgc ctttaccaaa gttcatatcg ttgtggctgc ccagacggaa gccggcacgc
     2221 acatgttgtg cactgccatg cccgaacttc acataaccgg cgttatcctg gccgtcatcc
     2281 agggagagcc cctgccaggt gtactgcagt tgtggctcca gcatcagatt gtcagtgata
     2341 ctgaagggca gaccggtttc cagtgagccc agccagcccc agccccgggc gcggaagtcg
     2401 ttattgtccg atgatgcttt catgctgtgg cgggttccct gtgccacaat gtcagcccac
     2461 aggccggagg atgtgtgtac cagattcagg tatccgccca gactgccggc atcatcccgg
     2521 accgtgccgg cgcgggagcc gtcatcatcc ttaacatcaa cggaagaatg gcctgcagca
     2581 ccatacaccc ctgtcgtcag agacataccg gcaacctctg ttctgagcag gtcaccctcc
     2641 agacggacga agccatagct gccgctgctt tccggcgtgg ccccacgggc aataccgccg
     2701 ttgttatcgt gcccgagatg accgccctga atgctgagac ggacgctgtt attttcaccg
     2761 tttacaccgg tctgatggct gcgggagcca gccagaatcc ggtcatagtc cattgcctgt
     2821 gtcagcatgg aggcatacag ggggacttca gcacgataag cattttcact gcgcaggtac
     2881 cagtcttcat cgctgtcacg gttcagggtg tagttaaagg cgccggcctg aagcgggcgg
     2941 ctcagggcaa acgcaccttc ttctgtggtg gcaccattct gtgcatccac aacccggata
     3001 ccctgtccgg tggttgccac cccgaggtta ctgtttccga catttgtgaa cgcaagccag
     3061 gttttgccgg ttgcctggcc accattaatc accagctggt cagaggcatt gctgccatca
     3121 aggcgaacac gcatattgat ggtgcccccc tgaccggtga ggttactggt ggtcagttta
     3181 tggaacgtta ccggggagtc gccttttgca acagcacggc tcagcgcggc atccggtgtc
     3241 gtctgattat caaagtgaat ctctcctgcg ttaacaatat cgccggtact gaccacatca
     3301 ccattcagta ccatgctggc gcctttactg agctgtgttc tgccactcag acttcccccg
     3361 gcagccagcg tcagtgttcc acgatatccg acagtggtgt taccggcctg tcccccggca
     3421 ttaaccgtaa agctgccgcc attttccagc agcaggccgc tggcctgacc ggagatacca
     3481 tcaatactga acttaccgct ggcattggtc ccctcaacag tggcaccact gtctgcaatc
     3541 agggcaccac cggatgtcat ggtgacacct gtcgccttac cgccggcaga cactgccagg
     3601 gtaccgccgt catccaccag ggtattctgt gctgaatggc tctccagtat atccagacga
     3661 ccgccggatt ccagaacaac accgtcagcc ttaccgtttt ccaccgtgaa attccccaga
     3721 cggttgctgc caagaacagt tgctgcagta ctggtgacca gtgcgcctcc ctgcttcagg
     3781 gtgacattcg tggctgtacc accggcgttc acctgcagtt tgcctttctg gttaacggtg
     3841 gtgaaatccg ccagaccacc ttccttgata atctgccagc cgtcactgtt tacaacagtg
     3901 ccagacgccg taccgtcgtt gtgcacatac tggtaccccc cattcagcgt ggtatccagt
     3961 gcgtgaccat gtacagtctg gtcgccgccg gcataaacca cagtggtatt tgccgttcct
     4021 tcagcagcca caatctgacg accattttta ttgatggtgg tgcgtacggc atctccgtag
     4081 acggtctgcc cggtatcacc attttccgca tccggtcccc cttccgcgcc ggtattcaca
     4141 accgtgtcgg ttgccactgc gccggatttg atggcctgcc agcccttctc attaatgacg
     4201 gtacccgttg caatcccgcc ttcatgtacc cactgctcac cgccgttcag cgtggtgttc
     4261 actgcctgcc cctgaaggct ctgtccgcct ccggcactga taaccgtgtc tgaaacgctt
     4321 cctccggcat tcactctctg aagaccacca ccggtgacag tagtgttgtt ggcgataccg
     4381 ccattttgta tccattgtcc gccggtattg gcctcgttat ccggcccata ctccagcccg
     4441 gtactgatgg tcattccgtt ggccgtaccg aggacaatct ggttgtcatg atttgtcagt
     4501 gttccgtcgt tcacggtttc tcccgcctgt acaaccgtgt cagcagccag tgccgggact
     4561 gatgtgacag cagcaagaga cagtgcaacc gccacaccgg cgcgttttcc ccgtgagcgg
     4621 gccagttcgg aggccaccac cagggtgccc gtaatgtgat tccataccag cctgtagctg
     4681 gtgttcagat gtcgtttcat cagcttttcc ttacagaggg tgaataaaaa agccggtacc
     4741 cacaaatgtg gataccggca ggtacaaaac agcatggtca gatacagtgg tttttcccgg
     4801 actagactgg ccccctgaat ctccagacaa ccagtatcac ttaaataagt gatagtctta
     4861 atactagttt ttagactagt cattggagaa cagatgattg atgtcttagg gccggagaaa
     4921 cgcagacggc gtaccacaca ggaaaagatc gcaattgttc agcagagctt tgaaccgggg
     4981 atgacggtct ccctcgttgc ccggcaacat ggtgtagcag ccagccagtt atttctctgg
     5041 cgtaagcaat accaggaagg aagtcttact gctgtcgccg ccggagaaca ggttgttcct
     5101 gcctctgaac ttgctgccgc catgaagcag attaaagaac tccagcgcct gctcggcaag
     5161 aaaacgatgg aaaatgaact cctcaaagaa gccgttgaat atggacgggc aaaaaagtgg
     5221 atagcgcacg cgcccttatt gcccggggat ggggagtaag cttagtcagc cgttgtctcc
     5281 gggtgtcgcg tgcgcagttg cacgtcattc tcagacgaac cgatgactgg atggatggcc
     5341 gccgcagtcg tcacactgat gatacggatg tgcttctccg tatacaccat gttatcggag
     5401 agctgcccac gtatggttat cgtcgggtat gggcgctgct tcgcagacag gcagaacttg
     5461 atggtatgcc tgcgatcaat gccaaacgtg tttaccggat catgcgccag aatgcgctgt
     5521 tgcttgagcg aaaacctgct gtaccgccat cgaaacgggc acatacaggc agagtggctg
     5581 tgaaagaaag taatcagcga tggtgctctg acgggttcga gttccgctgt gataacggag
     5641 aacaactgcg agtcacgttc gcgctggact gctgtgaccg tgaggcactg cactgggcgg
     5701 tcactacggg cggcttcaac agtgaaacag tacaggacgt catgctggga gcggtggaac
     5761 gccgcttcgg caacgagctt ccggcgtctc cagtagagtg gctgacggat aatggttcat
     5821 gctaccgggc taatgaaaca cggcagtttg cccggatgtt ggggcttgaa ccgaagagca
     5881 cggcggtgcg gagtccggag agtaacggca tagcagagag cttcgtgaaa acgataaagc
     5941 gtgactacat cagtgtcatg cccaaaccag acgggttaac ggcagcaaag aaccttgcag
     6001 aggcgttcga gcattataac gaatggcatc cgcatagtgc actgggttat cgctcgccac
     6061 gggaatatct acggcagcaa gccagtaatg ggttaagtga taacaggtgt ct
//
LOCUS       DAZFVO020000233         5980 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFVO020000233 DAZFVO020000000
VERSION     DAZFVO020000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.235"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            52..1050
                     /locus_tag="ACHKNB_RS23565"
                     /old_locus_tag="pgaptmp_004713"
     CDS             52..1050
                     /locus_tag="ACHKNB_RS23565"
                     /old_locus_tag="pgaptmp_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HGU4198087.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVVVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQY
                     QLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(1100..2848)
                     /locus_tag="ACHKNB_RS23570"
                     /old_locus_tag="pgaptmp_004714"
     CDS             complement(1100..2848)
                     /locus_tag="ACHKNB_RS23570"
                     /old_locus_tag="pgaptmp_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HGU4198088.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2848..3918)
                     /locus_tag="ACHKNB_RS23575"
                     /old_locus_tag="pgaptmp_004715"
     CDS             complement(2848..3918)
                     /locus_tag="ACHKNB_RS23575"
                     /old_locus_tag="pgaptmp_004715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4198089.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3908..5359)
                     /locus_tag="ACHKNB_RS23580"
                     /old_locus_tag="pgaptmp_004716"
     CDS             complement(3908..5359)
                     /locus_tag="ACHKNB_RS23580"
                     /old_locus_tag="pgaptmp_004716"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HGU4198090.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5370..5816)
                     /locus_tag="ACHKNB_RS23585"
                     /old_locus_tag="pgaptmp_004717"
     CDS             complement(5370..5816)
                     /locus_tag="ACHKNB_RS23585"
                     /old_locus_tag="pgaptmp_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HGU4198091.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1569 a         1513 c         1515 g         1383 t
ORIGIN      
        1 ggcaattaaa tgaaaaggaa tttattatct tccgctatta tagtcgccat catggccctc
       61 ggtctgacgg gttgtgatga taaaaaagcc gaaacagaaa cgcccccgcc tgccaatagt
      121 caacctgccg caccagctcc tgaagctccc gttgcaaaag cagaagctaa acctgaaaca
      181 cctgcgcaac cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc
      241 aagtgctata acaagttaca gatccctgta cagagcagtc tggcgcgtta tgctgactgg
      301 ctgaaagatt ttaaacaggg gcctaccggt gaagagcgta ctgtttatgg catctacggc
      361 attagtgaat ccaacctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg
      421 cctgcgctgc aaccaattga tggcgtggtg gtgagttata tcgatgctgc cgtggcgttg
      481 ggtaatacca ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg
      541 tttgcgaaag ggaaaacgct gcaccagaca ttcttaaaaa atctggaagc ctttgaacct
      601 gtagcagaat cttatcatgc ggcgattcag gaaattaacg ataagcgcca gcttgcggaa
      661 ctgaaaaata ttgaagaaag agaagggaaa acattccact actactctct ggcagtcatg
      721 atttcagcga aacaaattaa taatctgata tcgcaagaga agtttgatgt tgatgcggca
      781 atgaagaaag tgtctgaact ggaaacgcta gtggcgcagg ccaaagaagc ggataaaggc
      841 ggcatgaatt tctcgtttat taattcggca ggccagtatc aactcgaggc taaaaaatac
      901 gttcgccgca tcagagataa agtcccgtac tctgactggg ataaagagca acttcaggac
      961 gcaaactcaa gctggatggt cgaagactct ttcccgagag cattacgcga gtacaacgaa
     1021 atggttgatg actataatag cctgcgttaa acgtttttga tgataaatgc agtgtcggat
     1081 gcggcgcatc cgacacagtt caccccttgt gaagtaactc cataaatgcc tcatatcctg
     1141 ttaacccggc aataatctcc gccggatgct gacacaatat gcttgccaga tagctaaata
     1201 tttttagctc atgacgcgca tcggcggcgg cgcaggcgat caagacatgg ttaatgactt
     1261 cgttattcac ctcaactggt tgggcgaggg taataaaaaa tccacgaaaa cgtcgctcct
     1321 gttcgctcca gcaatgtggg atggcgaggc gattaacaat caggttttca ccttcgcctt
     1381 cgcgggcgat gatgcgttgt gcttcatcgg cggtaatatg gtgttgtgcg actaattgcg
     1441 cacaaatttg ccgggtaata tgttgccagc tttcaccgcg tacattcgaa taatgaaaac
     1501 ttccgggggc agagaaaaaa cgctccggtt gctggcgaat aaatgccgtc gccagaaaat
     1561 gttttatttg ttcgataccg gcagccgtaa tgatattttt tacggtgatg taattattta
     1621 ccgcgtcatc cagtaaataa tggctgttgt taatgatcaa taacggctca atctcttcgc
     1681 gaatggcaac aagttcgctt aagctacggg caataatcac ccgacaatgt aaaacatcgc
     1741 gctcaatggc gagctgatta atagtggcaa tcgcgttctg gtccgagagc aaaatgatcg
     1801 gctggcgttc gttttgatgc cgttccagcg cacaggcaaa atacaaaccg atcagatcgc
     1861 tgtcgaaaag gggaatatcg agctgttcac gcagtagcgt aataaagtgc agactcatat
     1921 caaaggccgc aggccaggcg gctttcaggt tattcatgct gctttggcgg tgctcagcaa
     1981 tccacaccgg tgcggcaagg caacgtaata aatgcccggt aatgttctca accagctgcg
     2041 tatcaataat attcaatgac gcgatgcccg gcaccctttg cagcaaccgc tgcacattat
     2101 cggcagaaat tatctgcgct tgctgatgct gtttctctat caaacctgtc agggttctta
     2161 cagcgttatc accaagaaac agaccgctat gttcaacgat ttgttttatc tcgtcaccgg
     2221 gatattgcgg ccactcatcg gtcagttgat tacgcagggc gtaaatcgcc agaatcaggc
     2281 tggagagata atcaccctgc atgagcggcc agcggtgttg gttgtcgcag gccgtggata
     2341 aatgctgaag gttgtctcgt gttacgcccg ccagcggaat taaaaacgga tctttgcgca
     2401 gcaagttcgc cagcaagata acgcgcttct cttctgtctc atcaatgaaa tggcccaaac
     2461 cggggcggct ggccaggcaa caagcgcgtt cataacgctg ttttaaccgg ggcagacgct
     2521 ctgcgaccca cgtttctggc aaattaagcg ccgaggcgag ttgcgcacgg ggagtaaagg
     2581 tattcagtaa taacagtgcc agcagccgat cgtcgttatc gtgcttttgc agcaactgaa
     2641 aaaagctgcg gcgctcgaag atttccagct gatagcccgc actaccactg gcggaaatgc
     2701 gggctttgcc gttaagggta aagttgagat agtcaatatc acgcaggatg gtcctgcctg
     2761 aaacgccagt ctgttgcgcc agctcaccag gcgtgcgcgg ctgttgctcc agcagatcga
     2821 caatctttaa ctggcgttcg tttagcatta atccacctga cggaactggc taaacgcgtt
     2881 gactttctcc gcagttaagg tggtcagaaa accccgtatc agcgtgagca gagcatcgta
     2941 atcggctttt gaaatcatac cgctgttggc gtgcagataa cgagttggca gacacagcgc
     3001 gacaaccgga cgcccgccgc ccatcacgtt gtagcgcccg ccatccgtcg ctccggtttt
     3061 catggtggaa aattgcagcg gtaaatcatt ctgcgtggca cagtttttaa acgctgctac
     3121 cagtttctgg ttggggaagt agcgcttgtc aaacagcatc agccccggcc cctggcccag
     3181 tttcagcggg tatttaatgt tatcaatgcc cggaacatca cctgcgacag cggtatccag
     3241 cacaatcacc acatccggtt taatgtgttc cgccgaggtt tgtgccccgc gtagcccaac
     3301 ttcttcttcc acgctgccga cgccatacag cgtaatttcg ggattattca ccgtttgcaa
     3361 tagttcagcc atcattgcgc agccgatgcg gttatccagc gccttgccga ccactttatc
     3421 ttcgccccag caggcaaaat tggcttccgg gctaataaaa tcgcccatcg ccacaccgcg
     3481 tttttccacc tcatcgcgac tgttcgcgcc aatatcaatg aacatctcat caaatgacag
     3541 cggctgttgc ttttgctttt ccgttaacgc atgaggtgcg acggaaccaa tcacaccagg
     3601 gattttaaat cccttgtgtg tgcgtatggt tacccggtgg ttgagcattg actgattcca
     3661 ccagccacca atggtggtga aacgcagaaa accgctctcg tcgatgtggg tgaccataaa
     3721 gccgacttca tccatatgcc cgacaacggc aacttttgga cctttattcc ccttacgggc
     3781 aacaaagctg cccagaccat caaaggtgat ctcattaacg caaggttcca gcgtgtttat
     3841 cagaatgtcg cgaacttcct gttcatcgcc gctgacggcg ctggcttcgc acaactgttg
     3901 cagtaactca atgttcatgg acaagctcct gttgcgcggc tttcgctttg cggcgtaacc
     3961 acaatccttt caggacgata atgaatgaaa tgttcagcgc gaggccaatc gccagcacca
     4021 gataaaacga acccaccggc gacatcaggc cgatcagcgg gtcaaagata cccaaaccag
     4081 gtgccagacg tttgattccg aaagcgatca ccagcacgcc ggtaatgcca cccgccagcg
     4141 tattggcggt aatcatcggc aacggagctg ccagaacgta aggtatagcc ggttcggttg
     4201 cgactgtggc accgaccaca atggcactac tggcagcctc tttttcctgt cgggtaaaca
     4261 gtttgggcgc aatgaacgtc gcaagacccg ccgcaacggg cggcatcaac gcgacaacgc
     4321 cgacgatggc gtaccagtca taaatgtgtt tttccagcag tgagaagcag aagaaccagg
     4381 cggttttatt gatggggccg cccatatcga acgccagcat cgcaccgacc agaaacgctg
     4441 ccgcaaactt cattgatgga ggaatggtat tcaggaagtg gagcagcccg cccatgaggt
     4501 cggacatcac cggcccgata acgtagtaag tgagcacgcc gaaaaccagc agagtaacga
     4561 acgggatcag catggaaccg agcagcggtt gtaatgcttt gccaaggcgc actttgcgga
     4621 accagaagac gaaatagcca atcgccagcc ccagcaccac cgcgccgagg aatcccgcac
     4681 ccgactgggt gccgagcagc gctttgtcgt tcgccagata gcaaaccaga aacgccggag
     4741 cgaaagccgg tttatcggca atcgacgagg caatataagc gcccatgatg gggatcataa
     4801 aggtgaagcc aaggtaaccg atggattcaa ctacccaggt aaacgacggc gcgcctttcg
     4861 acatatcggt atacggcaaa ccgaactgca ccagcatgtt ggcaagcgcc accaggatac
     4921 caccgccgat gacaaacggc agcgccgcag agacgcccgc catcaggtgg ctcatcacgc
     4981 tgccgctttg cacttcctgt ttgccaagct tcacgccgct atctgcagca aaaagatgcg
     5041 agtttgttgg taattcactg aaaatctgat cgatattttt cagcgcctgg gagatggcaa
     5101 tctcataaac cttcttcccg gcaaaccgcg cgcgatcgtc accgctcagg ccacgcccgg
     5161 tagcgagaat gacgtaatcg gcagcggcaa tctcttcgct ggataagcgg ttttcgacgc
     5221 cgcttgatcc ttgagtttca actttaatgg tatgaccgag agaacgggct ttctgttcca
     5281 gggcttccgc caccatgtag gtgtgagcga tcccggcggg gcagttggtg atcgcgacaa
     5341 tacgtaagga tgactccatc gccacttcct tatgagctga gggtttgatt taacaaggca
     5401 agcacctggt cggtatcgcc ctgttgcagt tgatgaataa attccttgtg aataattttg
     5461 cgacacagtg tgccgatgat tttgacctga tcctcttcgc cgctttgcgg cacgccgagg
     5521 cagatccagc aattgacgtc ttcgccatcg ctggctttcc agtcaatagc ctgcgctttg
     5581 cgggcgaata acacgaacgg ttgtttaacg caggcgcttt taccgtgcgg cacggcgatg
     5641 ccggaaccaa atccagtaga gtgcattttt tcgcgcagca gcagggtttg caggaactgg
     5701 tgtgagtcgg tgataaaacc gttttgtaac gccattgtcg ccaactgctt cagaatggaa
     5761 taagcgccat tgccctgaat attcaggtca atacagcttg cagtaagagc tgccattaat
     5821 cggactccat gaaatatatt ttaatcaatt gtaatggtgg agcgattaag tgatgagagg
     5881 cgatatgtcg gtaaacaatg acaatttgag acagagtgaa aggtcagatt tgcggagtaa
     5941 tgcacataat ggttatttaa ataaaccaca tgaatcatta
//
LOCUS       DAZFVO020000234         5741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFVO020000234 DAZFVO020000000
VERSION     DAZFVO020000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.236"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            78..1628
                     /locus_tag="ACHKNB_RS23590"
                     /old_locus_tag="pgaptmp_004718"
     CDS             78..1628
                     /locus_tag="ACHKNB_RS23590"
                     /old_locus_tag="pgaptmp_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HGU4198092.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPGELQNACS
                     YIIDPDSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNKYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLENHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1655..2107
                     /locus_tag="ACHKNB_RS23595"
                     /old_locus_tag="pgaptmp_004719"
     CDS             1655..2107
                     /locus_tag="ACHKNB_RS23595"
                     /old_locus_tag="pgaptmp_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HGU4198093.1"
                     /translation="MYYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2260..3534
                     /locus_tag="ACHKNB_RS23600"
                     /old_locus_tag="pgaptmp_004720"
     CDS             2260..3534
                     /locus_tag="ACHKNB_RS23600"
                     /old_locus_tag="pgaptmp_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HGU4198094.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGEPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVGNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3527..5623
                     /gene="mchF"
                     /locus_tag="ACHKNB_RS23605"
                     /old_locus_tag="pgaptmp_004721"
     CDS             3527..5623
                     /gene="mchF"
                     /locus_tag="ACHKNB_RS23605"
                     /old_locus_tag="pgaptmp_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HGU4198095.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFQVETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMVGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     SMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPRILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIISI"
BASE COUNT         1640 a         1075 c         1416 g         1610 t
ORIGIN      
        1 ggaagaagct gtcaggacac ttacttgctc ttttatgaac actcttttat gaaaattaaa
       61 acggagaata ctgccagatg agtcatcagt gttcactttc tgaactgaat gaaaacctgg
      121 tgcctttcac tgccaggcag atcaagtcct cattaatctg gtgtgcagag gatgtcagaa
      181 atccaggcga gctgcaaaat gcctgcagtt atattatcga tcctgacagt acggcttctg
      241 ccaaagtgtt ccatgcagag cgctatggtg gcagtggtat tcagcgtaat ggaggtggtg
      301 cacgttgtgg gtttgatggt aactaccagg ttaaaggaat aggaagtaat ccgttggttg
      361 gcgaaggtac tgacgaacgt cattctaatg gtgcactcgg cgctgttcat gcaatatatg
      421 aggctttgtg gggagaagta ctggctcaaa tattacctta tagtgctgtg cgggttcggg
      481 cggttttact tacagatctc tatactgaaa aggcatttga gcgctccggt aggaaatcac
      541 gaagagccct gttggtacgt gagcctgttg ttcgcccggc gcattttgaa cgggcacctt
      601 actttcaagt aaaaccggag tattccagtc tattaattca cgatgcctgt cgggttagat
      661 ctgtgatcca caagctgcca ggatatctac ctgtaccacc ggaagaaatt gatgctgaag
      721 cacgaactga tccccggatt tattgcattg aggggttatg tgaattggca cgtcgtgagg
      781 cctggcaaat ggcattttgt cgaacacgtt tcctgagatt gacaacttct ccttctaata
      841 ttgcaatgga tggcagatta atggatttta acggactcag ttgtctgttt ccgggagatt
      901 ccccagctga ttttgggtat aaactaagat tagctgaact ggcaaaagaa ccgatggtac
      961 ttatgcaagg gctgtctgat ctctgcttgt atatcggaaa atatatgttt gaccctgact
     1021 tcactcttgc agcccgtttg aaggttgagg agatatttca gaaaactttt catgaagcat
     1081 gttattactg ttatctagaa ctgttgggta ttcctggaga atttataaca caaaaagaga
     1141 tacctgatat attgaaagaa ctggttaaca gttttgttgc attactcaat aaatactgtg
     1201 agaaatcaca tgcccaagat attgtcaatc aggatggttc accattgcaa aagttggttg
     1261 tgacgctaat ccatcatagg cataatcaaa agcaggcact gaatagtagc atcaagaatg
     1321 atgtttattt cacagttgca caacagtgtt tttcccaggc catccactgg ctgacgcaag
     1381 gcagtaccag acgtcagata aatgcctcat tactcctgaa agaaattgaa catcatacca
     1441 tgaaaaggct gcaacccagg gaagagctga ggaaagagaa tatgtgcgaa aaaattgcca
     1501 tcctgctgga gaatcatggc gatgatcccc tttttttaca agaagcaatt tctgatatga
     1561 aaaattttat gcttaagttt tccagagatg catttggata tcttgaaccg ataagaaaca
     1621 cagtgtaata aatatataaa gggaaatagt aatcatgtat tatataaggg aaaccatcag
     1681 aggaaaagat gaatggactg tttatgaaca gatcggtttt gcggtcagtt gtatgctcta
     1741 caatcgtaat tacagtctgt atccggtgtt aaccattcaa tactggactg aatatgcgat
     1801 acagcataat cagattaaat tcctgtttga ttcacgaggt tttccactgg cgtatattac
     1861 ctgggcatat cttgaggctg atacagaagc gcgcctgctc agggatccag aattcaggtt
     1921 gcatccgtct gaatggaatg aagatggaag gatctggatt ctggatttct gttgtaaacc
     1981 aggctttggt cgaaaagtta ttgactatct catacagctt cagccatggg gagaaggaga
     2041 agtacgatgg ttaagcaggc gaaagaaaat tgtgacatac atccctgagc ggctgcataa
     2101 aacgtagtac ctctgaagat acagagataa ttgtaaatta cggggtaaat gcatcgctga
     2161 tactattttg acaggactct gtattttctg gctttgatta tagcatttcg gttaaccagt
     2221 gaaggtatta caggaaaagt gtgagtaaga ggagctattt tgtttcgtca ggatgcttta
     2281 gaaaacagaa aaatgaagtg gcagggacgg gcaatattac ttcccggaat accactatgg
     2341 ttaatcatgc tgggaagcat agtgtttatt acggcatttc tgatgttcat cattgttggt
     2401 acctatagcc gccgtgttaa tgtcagtggt gaggtcacaa cctggccaag agctgtcaat
     2461 atatattcag gtgtacaagg atttgttgtc aggcagtttg ttcatgaagg acagttgata
     2521 aaaaaagggg agcctgttta tctgattgac atcagtaaaa gtacacgtag tggtattgtc
     2581 actgataatc atcgtcggga tatagaaaac cagctggttc gtgtgggcaa cattatttcc
     2641 cgtctggaag aaagtaaaaa aataacgctg gataccctgg aaaaacaacg tctgcaatac
     2701 acagatgcgt ttcgtcgctc atcagatatt atacagcgtg cagaggaagg gataaaaata
     2761 atgaaaaaca atatggagaa ttacagaaac tatcaggcaa aagggctgat taataaagat
     2821 cagttaacta accaggtggc attatattat cagcaacaaa acaatcttct cagcctgagc
     2881 ggacagaacg aacagaatgc cctgcagata accactctgg agagtcagat tcagactcag
     2941 gctgcagatt ttgataaccg tatctaccag atggaactgc aacggtacga gttacagaaa
     3001 gaactggtta acactgatgt ggagggcgaa atcattatcc gggcgttgac tgacgggaaa
     3061 gttgactccc tgagtgtcac tgtcgggcaa atggtcaata ccggagacag ccttctgcag
     3121 gttattccag agaacattga aaactattat cttattctct gggtcccgaa tgatgctgtt
     3181 ccttatattt cggctggtga caaagtgaat attcgttatg aagcctttcc ggcagaaaaa
     3241 tttgggcagt tctctgctac ggttaaaact atatccagga ctcctgcgtc aacacaggaa
     3301 atgttgacct ataagggagc accacagaat acgccgggcg cctctgttcc ctggtataaa
     3361 gtcattgcga tgcctgaaaa gcagattatc agatatgacg aaaaatacct ccctctggaa
     3421 aatggaatga aagccgaaag tacactattt ctggaaaaaa ggcgtattta ccagtggatg
     3481 ctttctcctt tctatgacat gaaacacagt gcaacaggac cgctcaatga ctaacgggaa
     3541 tttcagacaa attataaatc agcttgatat gcgctggcga cgtcgtgttc cggttattca
     3601 tcagacggag accgctgaat gtggactggc ctgcctggca atgatatgcg gtcattttgg
     3661 taagaatatt gacctgatat ctcttcgccg gaagtttaat ctctcggccc gtggagcaaa
     3721 ccttgcagga atcaatggaa tagcggagca actggggatg gtcacccggg ctctttcact
     3781 ggagctggat gaacttggtg ccctcaaaat gccgtgtatt ctccactggg atttcagtca
     3841 cttcgtcgtg ctggtcagcg taaagcgtaa ccgttatgta ctgcatgatc cggccagagg
     3901 cagaagatat ctcggtcggg aggaaatgag ccggtatttt acgggcattg cacttgaggt
     3961 ctggcccgga agtgaattcc aggtggaaac ccagcagacc cgcataagtc tccgttcact
     4021 gattaacagt atttacggta ttaaaagaac actggcgaaa attttctgtc tgtcagttgt
     4081 aattgaagca atcaatctgg taatgccggt gggaactcag ctggttatgg atcatgcgat
     4141 tccggcgggg gacagagggc tgctgacgct tatttctgct ggcctgatgt tctttatatt
     4201 gctcagggcc gcggtgagta tgctgcgtgc atggtcctca ctggttatga gcacgctcat
     4261 caatatacag tggcagtcgg gtctgtttaa ccatcttctc agactgccgc tggcgttttt
     4321 tgaacgccgt aaattaggtg atatccagtc gcgttttggc tcccttgaca ctttgagggc
     4381 cacctttacc acctgtgtgg ttggggcaat catggacagt attatggttg taggtgtttt
     4441 tgtgatgatg ctgttatatg gaggatatct tacttggata gttctcggtt ttaccatggt
     4501 ttacgttctt attcgtctgg tgacatacgg ctattaccgg caaatatcgg aagaaactct
     4561 tgtcaggggg gcccgggcca gctcctattt tatggaaagc ctgtatggta ttgccacggt
     4621 aaaaatccag ggtatggtcg ggatccgggg aacacactgg cttaacctga aaatagatgc
     4681 gatcaattca ggtattaagt taactaggat ggatttgctc ttcgggggaa taaatacttt
     4741 tgttgccgcc tgtgatcagg tggcgatttt atggctgggt gcaagccttg tgatcgataa
     4801 tcagatgaca atagggatgt ttgtggcatt tggttctttt cgtgggcagt tttcggatcg
     4861 ggttgcttcg ctgaccagtt ttcttcttca actgagaata atgagtctac ataatgagcg
     4921 cattgcagat attgcactac atgaaaagga agaaaagaaa ccggaaattg aaatcgttgc
     4981 tgacatgagc ccggtttcac tggaaaccac tgatttaagc taccggtatg acagccagtc
     5041 agcacaggta ttcagtggtc tgaatttgtc tgtggctccg ggagaaagtg tggctataac
     5101 tggtgcctcc ggtgccggaa aaaccacatt aatgaaagta ttatgtggac tgtttgaacc
     5161 agatagtgga aaagtactgg ttaatggcac ggatatacgt caacttggaa taaataatta
     5221 tcacagtatg atagcctgtg ttatgcagga cgaccggcta ttttcaggat caattcgtga
     5281 aaatatctgt gggtttgcag aagaaacaga cgacgaatgg atgacagaat gtgccagagc
     5341 aagtcatatt catgatgtga taatgaaaat gccaatgggg tatgaaacgt taataggtga
     5401 actgggggaa ggtctttccg gcggtcaaaa acagcgtata ttcattgccc gagctttata
     5461 ccggaaacct agaatattat ttatggatga ggctacaagt tctcttgata cagaaagtga
     5521 acgtttcgtg aatgctgcca taaaaaaaat gaatatcacc cgggtgatta ttgcacacag
     5581 agaaactacg ttgagaactg ttgacaggat tatttctatt taaaatccac tggtgtaact
     5641 ttgtaaggag ttttgtcgat gggggaggtt aagaaggata taaaaataac agtgattgct
     5701 tttgttatca attatctgtt cttttatatt ccggtgtcat t
//
LOCUS       DAZFVO020000235         5575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZFVO020000235 DAZFVO020000000
VERSION     DAZFVO020000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.237"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            181..813
                     /gene="yfdX"
                     /locus_tag="ACHKNB_RS23610"
                     /old_locus_tag="pgaptmp_004722"
     CDS             181..813
                     /gene="yfdX"
                     /locus_tag="ACHKNB_RS23610"
                     /old_locus_tag="pgaptmp_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HGU4198096.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNNDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1326..2576
                     /gene="frc"
                     /locus_tag="ACHKNB_RS23615"
                     /old_locus_tag="pgaptmp_004723"
     CDS             1326..2576
                     /gene="frc"
                     /locus_tag="ACHKNB_RS23615"
                     /old_locus_tag="pgaptmp_004723"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HGU4198097.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            2630..4324
                     /gene="oxc"
                     /locus_tag="ACHKNB_RS23620"
                     /old_locus_tag="pgaptmp_004724"
     CDS             2630..4324
                     /gene="oxc"
                     /locus_tag="ACHKNB_RS23620"
                     /old_locus_tag="pgaptmp_004724"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HGU4198098.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQILFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYVIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            4394..5338
                     /gene="yfdV"
                     /locus_tag="ACHKNB_RS23625"
                     /old_locus_tag="pgaptmp_004725"
     CDS             4394..5338
                     /gene="yfdV"
                     /locus_tag="ACHKNB_RS23625"
                     /old_locus_tag="pgaptmp_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HGU4198099.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            5412..>5575
                     /locus_tag="ACHKNB_RS23630"
                     /old_locus_tag="pgaptmp_004726"
     CDS             5412..>5575
                     /locus_tag="ACHKNB_RS23630"
                     /old_locus_tag="pgaptmp_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HGU4198100.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGP"
BASE COUNT         1527 a         1184 c         1300 g         1564 t
ORIGIN      
        1 gaatagttct ctgcgacagg aagcagattc ctacaattgt aagactaaaa tacttcttgc
       61 gataataact acaactgtaa gataaccctt tcaaaatgac cgttgctctc tgatttctca
      121 tttcatgctc acccaatatg atggcggcgt tttctaaaac tgttaaagaa tgaggtaagt
      181 atgaaacgtt taattatggc tacgatggtc acagcaattc tggcatcttc aactgtatgg
      241 gctgctgata atgcaccggt agcggcacaa cagcaaactc agcaagttca gcaaacccag
      301 aaaacggcag cagcagagcg tatctctgaa caaggtttat atgcgatgcg tgacgtgcag
      361 gtagcgcgcc tggctctgtt tcatggcgat ccagaaaagg caaaagaact gaccaatgaa
      421 gcttccgctt tgctgtcaga tgacagtacc gaatgggcaa agttcgctaa gccaggtaaa
      481 aagaccaatc tcaataatga ccaatatatt gtcattaatg catctgtcgg aatttctgag
      541 agttatgtag caactcccga aaaagaagcc gcgataaaaa ttgccaatga aaaaatggcg
      601 aaaggggata aaaagggcgc aatggaagaa cttcgcctgg ccggtgttgg cgtaatggaa
      661 aaccaatatc tgatgccgct taaacagact cgtaatgcac ttgctgatgc gcagaaactg
      721 cttgataaaa agcaatacta cgaggcaaac ctggcgctta aaggtgcgga agatggaatc
      781 atcgtcgaca gcgaagcact gtttgtgaac taatgctgac aggcagtata ttgaggtaaa
      841 ggatttaact ccctccagcc gacaattttt tatgaccaca atacgacagg ttgtggtcat
      901 aaaattattt ggcgctcgcg attctataaa cacgcatttt atcttcctca aagtaaatcc
      961 caataatccc gtgtcatcac aaatcattta ttatgttatg ttttatggta cgctttaaaa
     1021 agcgtaatca tacccttact tgtaaatcac cggcgcttct tacagttgta agaataacat
     1081 cacaaatgtc gcgactctct ctgattacca cgaatcaata tttatcatat tcttcttttc
     1141 gtcctcacta cttaccatgc agaaaggaat tggaagtggc gatgaaatga ttttcagcac
     1201 ttgatacagg attttaaaaa tatcgagtgc agctacgacg atattttgcg ggttatttat
     1261 agaagatatt acgccttaaa taaacgctaa attaacacat atttaattaa ctaataggta
     1321 ttgctatgtc aactccactt caaggaatta aagttctcga tttcaccggt gtgcaatctg
     1381 gcccatcttg tactcaaatg ctggcctggt ttggcgctga cgttattaaa attgaacgtc
     1441 ccggcgttgg tgacgtaacg cgtcaccagc tgcgagatat tcctgatatc gatgcgcttt
     1501 acttcaccat gcttaacagt aacaaacgtt ctattgagtt aaataccaaa acagcggaag
     1561 gcaaagaggt aatggaaaag ctgatccgcg aagctgatat cttagtcgag aactttcatc
     1621 caggggccat tgatcacatg ggcttcacct gggagcatat tcaagaaatc aatccacgtc
     1681 tgatttttgg ttcgatcaaa gggtttgatg agtgttcgcc ttatgtgaat gtaaaagcct
     1741 atgaaaacgt tgctcaggca gcgggtggcg cggcatccac tacgggtttt tgggatggtc
     1801 cgccgctggt aagcgctgca gcgttgggtg acagcaacac cggaatgcat ttgctgatcg
     1861 gtttacttgc tgctttgctg catcgcgaaa aaacggggcg tgggcaacga gtcaccatgt
     1921 caatgcagga tgccgtattg aacctttgcc gcgtgaaatt acgtgaccag cagcgtctcg
     1981 ataaattggg ttatctggaa gaatacccgc agtatccgaa tggtacattt ggtgatgcag
     2041 ttccccgcgg tggtaatgca ggtggtggcg gtcagcctgg ctggatcctg aaatgtaaag
     2101 gctgggaaac cgatcctaac gcctatattt atttcactat tcaggagcaa aactgggaaa
     2161 acacctgtaa agccatcggc aaaccagaat ggattaccga tccggcatac agtacagccc
     2221 atgcccgaca gccacatatt ttcgatattt ttgctgaaat cgaaaaatac actgtcacta
     2281 ttgataaaca tgaagcggtg gcctatttga ctcagtttga tattccttgt gcaccggttt
     2341 taagtatgaa agaaatttca cttgatccct ctttgcgcca aagtggcagt gttgttgaag
     2401 tggaacaacc gttgcgtgga aaatatctga ccgttggttg tccaatgaaa ttctctgcct
     2461 ttacgccgga tattaaagct gcgccgctat taggtgaaca taccgctgct gtattgcagg
     2521 agctgggtta tagcgacgat gaaattgctg caatgaagca aaaccacgcc atctgatatt
     2581 aacgggggct tctggctccc aatttataaa aatttcgagg ttattaatca tgtcagatca
     2641 acttcaaatg acagatggta tgcatatcat cgttgaagca ttaaaacaga ataatattga
     2701 cactatttat ggtgttgtag gtattcctgt gacggatatg gcacgccatg cccaggcgga
     2761 aggcattcgt tatattggtt ttcgtcatga gcagtcggca ggctatgccg ctgcggcaag
     2821 cggttttctt acccaaaaac cggggatctg cctgacagtt tctgcgccag gattcctcaa
     2881 tggtttgacc gcattggcca acgcaacggt aaatggtttt ccgatgatca tgattagcgg
     2941 ctccagcgac cgcgcgatcg tcgacctaca gcaaggtgat tatgaagagc tggaccaaat
     3001 gaatgcggca aaaccgtatg ccaaagcagc atttcgcgtt aatcagccgc aggatcttgg
     3061 cattgcattg gcacgcgcta tccgggtctc tgtatcgggt cgccctggcg gagtttatct
     3121 tgatttgcca gcaaatgtcc tggccgcgac gatggaaaaa gacgaagcgt taaccacgat
     3181 tgttaaagtt gaaaatccat cgccagcatt attgccatgc ccgaagtcag tcactagcgc
     3241 aatttcgctt ttagcaaaag ctgaacggcc attaattatc cttggcaaag gcgcggcgta
     3301 ttcacaagct gatgaacagc ttcgtgaatt tattgaaagt actcagattc tattcctgcc
     3361 aatgtctatg gcgaaaggga tccttgaaga tacgcatcca ctttctgcgg cagctgcgcg
     3421 ttcgtttgcc ctggcaaatg ctgacgttgt catgcttgtt ggtgcacgac tgaattggtt
     3481 attggcacac ggtaaaaaag gatgggcggc agatacacag tttattcaac tggatattga
     3541 accgcaggaa attgacagca accgccccat tgctgtgcca gtcgttggtg atattgcatc
     3601 cagtatgcaa ggtatgctgg cagaactgaa acaaaacaca tttacgactc cactggtatg
     3661 gcgcgatatt ttaaatatcc acaagcagca aaatgcacaa aaaatgcatg aaaaattaag
     3721 tacagatact caaccattaa attactttaa tgcattaagt gctgtgcgcg acgtattgcg
     3781 cgagaaccag gatatttatt tagttaatga aggtgcaaat accctggata atgcacgaaa
     3841 tattattgat atgtataaac cacgtcgtcg tctggattgt ggtacctggg gtgtcatggg
     3901 catcggtatg ggctatgtca tcggtgctag cgtgacttct ggttctccgg ttgtcgccat
     3961 tgaaggtgat agtgcttttg gtttcagtgg gatggaaatt gaaacgattt gtcgatataa
     4021 cctgccggtg acgatcgtta tttttaataa tggcggcatc tacagaggag acggtgttga
     4081 tctcagtggc gctggtgcac catcaccaac ggatctgttg caccatgcaa ggtatgacaa
     4141 attaatggat gcgtttcgtg gcgttggcta taacgtcacc acgacagatg aacttcgtca
     4201 tgctttaacc accggtattc agtcgcgcaa accgaccatt attaatgtgg tcatcgaccc
     4261 tgcagcagga actgaaagtg gccatattac caaacttaac ccaaaacaag tcgctggtaa
     4321 ttaaatactg acatcgtctc ctcttgccaa ctcattatgc gatggtgagg ggacccttat
     4381 cggaaatact attatgctaa cattttttat tggcgattta ttgcctatta tcgtaatcat
     4441 gctgttgggt tattttagcg gcagacgaga aactttttca gaagatcaag cccgcgcatt
     4501 taataaactg gtattaaact acgctcttcc tgcggctcta tttgtttcta ttacccgggc
     4561 aaacagggaa atgatttttg cggacactcg tctgaccctt gtatcacttg tggttattgt
     4621 cggatgtttc tttttctcct ggtttggttg ctacaaattt tttaaacgta cccatgcaga
     4681 agcagctgta tgtgcattaa ttgcgggttc acctaccatt ggattcctgg ggtttgcagt
     4741 tctcgatcct atttatggtg attccgtatc aacaggttta gtggtagcaa ttatttctat
     4801 tattgttaac gcaattacta ttcctattgg tctgtatttg ctgaatcctt cctcaggagc
     4861 ggatggtaag aagaatagta atctgagcgc attaatttct gcggcaaagg agccagtagt
     4921 atgggcacct gttctggcaa cgatcctggt gttggttggg gtaaaaattc cggcagcatg
     4981 ggacccaacc tttaatctga ttgcgaaggc taactcaggg gtagcggtat tcgctgcggg
     5041 gttgactctg gctgcacata aattcgagtt cagtgccgaa attgcttata acaccttcct
     5101 gaagctgatt ctgatgccac tggcactgct tctcgttggt atggcatgtc atttgaacag
     5161 cgaacatctg cagatgatgg tactggcagg cgcattaccg ccggcattct ccggaatcat
     5221 tattgccagc cggtttaatg tctacacccg tactggtaca gcgtcattgg ctgtgagcgt
     5281 actgggcttt gtcgtcacgg ctcccttgtg gatttatgtc agtcgactgg tttcataatt
     5341 aatcatgtta ttgattttac ataaatgccg aagtgtcttt tcaccttcgg cttttttcaa
     5401 ggcataagca aatgacaaat aatgaaagca aagggccgtt tgaaggctta ttagttatcg
     5461 atatgacaca tgtccttaat ggacctttcg gaactcaact tctttgtaat atgggcgcaa
     5521 gggtaattaa agttgagccg ccgggtcatg gtgatgatac ccgcacattt ggtcc
//
LOCUS       DAZFVO020000236         5546 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFVO020000236 DAZFVO020000000
VERSION     DAZFVO020000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.238"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            156..1259
                     /gene="proB"
                     /locus_tag="ACHKNB_RS23635"
                     /old_locus_tag="pgaptmp_004727"
     CDS             156..1259
                     /gene="proB"
                     /locus_tag="ACHKNB_RS23635"
                     /old_locus_tag="pgaptmp_004727"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGU4198101.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1271..2524
                     /gene="proA"
                     /locus_tag="ACHKNB_RS23640"
                     /old_locus_tag="pgaptmp_004728"
     CDS             1271..2524
                     /gene="proA"
                     /locus_tag="ACHKNB_RS23640"
                     /old_locus_tag="pgaptmp_004728"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGU4198102.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2639..2714
                     /locus_tag="ACHKNB_RS23645"
                     /old_locus_tag="pgaptmp_004729"
     tRNA            2639..2714
                     /locus_tag="ACHKNB_RS23645"
                     /old_locus_tag="pgaptmp_004729"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2672..2674,aa:Thr,seq:cgt)
     gene            complement(2841..3179)
                     /locus_tag="ACHKNB_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /pseudo
     CDS             complement(2841..3179)
                     /locus_tag="ACHKNB_RS23650"
                     /old_locus_tag="pgaptmp_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3230..4186)
                     /gene="paoD"
                     /locus_tag="ACHKNB_RS23655"
                     /old_locus_tag="pgaptmp_004731"
     CDS             complement(3230..4186)
                     /gene="paoD"
                     /locus_tag="ACHKNB_RS23655"
                     /old_locus_tag="pgaptmp_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HGU4198103.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGGVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            complement(4196..>5546)
                     /gene="paoC"
                     /locus_tag="ACHKNB_RS23660"
                     /old_locus_tag="pgaptmp_004732"
     CDS             complement(4196..>5546)
                     /gene="paoC"
                     /locus_tag="ACHKNB_RS23660"
                     /old_locus_tag="pgaptmp_004732"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4198104.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
BASE COUNT         1239 a         1544 c         1566 g         1197 t
ORIGIN      
        1 aaatctgtaa taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac
       61 gccatgcttt gcttgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg
      121 atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg
      181 taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg
      241 ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga
      301 cgtcgggcgc gatcgccgcc ggacgtgagc acctggggta cccggaactg ccagcgacta
      361 tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg actgattcaa ctgtgggaac
      421 agctgttttc gatttatggc attcacgtcg ggcagatgct gctgacccgt gctgatatgg
      481 aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata
      541 tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaag gtcggcgata
      601 acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga
      661 ccgatcaaaa aggtttgtat accgctgatc cgcgcagcaa tccgcaggca gaactgatta
      721 aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc
      781 tcggaactgg cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta
      841 tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca
      901 tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga
      961 ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc
     1021 ttgaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc
     1081 gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc
     1141 gttacaacag cgatgcatta cgccgtattg ccgggcacca ctcgcaagaa attgatgcaa
     1201 tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag
     1261 gagcaggctg atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc
     1321 gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc
     1381 acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gtgccaatgg
     1441 ccttggcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc
     1501 cgatgatgtg cgccaggtgt gtaacctcgc cgatccggtg gggcaggtaa tcgatggcag
     1561 cgtactggac agcggcctgc gtcttgagcg tcgtcgcgtg ccgctggggg tgattggcgt
     1621 gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg
     1681 taatgcggtg atcctgcgtg gtggcaaaga aacctgtcgc actaacgcgg caacggtggc
     1741 ggtgattcag gacgccctga aatcctgtgg cttaccggcg ggtgccgtgc aggcgattga
     1801 taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct
     1861 gatcccgcgc ggcggggctg gtttgcataa actgtgccgc gaacagtcga caatcccggt
     1921 gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagcgcag agatcgctga
     1981 agcattaaaa gtgatcgtca acgcgaaaac tcagcgaccg agcacatgta atacggtaga
     2041 aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat
     2101 ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg ctggcgcagt tgcagacagg
     2161 ccctgcgaag gtggtggcgg ttaaagccga agagtatgac gatgaatttc tgtcattaga
     2221 tttgaacgtt aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg
     2281 cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa
     2341 cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca
     2401 gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat
     2461 ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc
     2521 gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg
     2581 cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc
     2641 cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc
     2701 tattatcggc accattaaaa tcaaagagtt accccatatt taaatacacc acgtttcctc
     2761 ctgtgccgca tttgtgccat tgtaaccttg gcaattcatc aaaatactgt tctgacatca
     2821 ggcagtgcag gtgcagctat ttaaaccaat tgctgccgcc attctttgac gtagtcaatc
     2881 agggcgcgga gctttggtgc aatattgcga cgctgtggga aatacagata gaagcccgga
     2941 aattgtggaa gaaagtcatc aagcagcgat acaagcttac cgctttcaat atatggcctg
     3001 aaagtttcct gagtggcaat tgttattcct ccgccggcaa gagccagcct caacatcaga
     3061 cgcagatcat tagtcgtaat ctgcggttca atcgcaaggt cgaaagttct cccgttttct
     3121 tcaaatggcc agcgataagg cgcaacctcc ggggactgac gccagccgat acacttatgg
     3181 gtatttcccc cggaggcgag aaagcgctct ccacgcccgg ccgcaaggat caggacgacg
     3241 ggggcaggca tgaatcctcc tcatgatgga gacgtacaga ggcgatttct gccagcacgg
     3301 agagtgccag agtatgcgca tcccgggctt tggggaatat cccgacgggg gcccggattt
     3361 gcgctgtctc ctccctggac catcccagct cgtggagctt ttgcagacgt aacgtgtggg
     3421 ttcgatggct gcccaatgcg ccgagataaa agggttttgc ttctcgcgcg gcctgcaatg
     3481 ctggcagctc ccggttgaga tcatggcaca gcaaaatgac cgccgtgtcg gtatcgatct
     3541 gagcgctggc tgaggccgga aaaagatcga agatatggct gtcatagcct gtggctgctg
     3601 caagactcgc ggttgcctgc gcctcaagag aacgtccgta aatcatcagc ctgacgcatg
     3661 gcctgaaccc cacctcaaag ccattgagat tccagcccgt tcgggtttgc gtgggcaggc
     3721 acaccaacga ttgtgcttgc ggatcgtagc gcagccccgc cggttttctc tgttccagac
     3781 ggttcagcac ggcgagcaga ggctgtgccg agcgtagttt atggagcgta agcgtgatcc
     3841 caccgccgca gggcagaacg atgtcaaacc acggcgaacc ttcgccatag cgaatttcgc
     3901 gatcgcggcc tgagcccatc atctccagcg cttcaaaagc ggcagcggcc tccacgccgc
     3961 cgccagagac aaaaccgcag taacgaccat cttcgcgaac caccatctgc gccccgagcg
     4021 ggcgcgccgc gccgccgtgt atctccacca gcgtcaccag caccgctcct ttaccggaca
     4081 ttagcgcctc tacggcgaag cgcagaatgg tccggtggtc atcggtgaga aacgcctgct
     4141 ctggcttatg ccagtgttcg tctttatcaa aaagcgggta tgacattgtt tctccttaaa
     4201 ccacatccgg cagcttatcg agcagcttat ccagagtgat gggataatcc cgtacccgaa
     4261 taccggtggc gttatacacc gcgttggcga tagccgcgct cacgccgcac aggcccaact
     4321 caccgacacc tttggccttc atcggggagg atatggggtc ggtatcatcc aggaaaatca
     4381 cctcctgttt tgggatatcc gcatggaccg gcacctcata ccccgccata tcgtgattaa
     4441 cgaagtagcc caaacggtca tccaccgcca gctcctccat cagtgccgcg cccatgccca
     4501 tagtcattgc gccaatgacc tggctgcgcg cagttttcgg attcaggatg cgtcctgcag
     4561 cacacacagc gagcatacgc cggacccgaa cttctcccgt cgcgctatgc acgccgacct
     4621 ccacaaaatg cccggcaaag gtcgactgct ggtactcttt gctcagtgtt ccgaattcaa
     4681 tgctctcttc cgctgtcagt ctgccgcctg cggtggcttc atgtagcgtg gcgcttcggg
     4741 taccgttggt aatcttgccg tcggcaaact gcgactgctc aggatcaaac ccgactgccg
     4801 aggcaatcat ttcgcgaagc ttcatacagg cggcgtaaac gccggaggtg gaggtattcg
     4861 cgccccattg tccaccagaa cccgcagaaa ccgggaaact ggaatcgccg aggtgaaccg
     4921 caacctgctc cagcggtacg ccaagcattt ccgctgccgt ctgggccaga atggtgtagc
     4981 tgccggtgcc aatgtcggtc atgtccgttt ctacggtaac ggtgccgttt tgttcgaggt
     5041 gaacccgagc acccgatttt tccagcagat tattgcgaaa gcccgccgca acaccgtggc
     5101 cgactagcca ctccccgtcg cgcacctgtc cgggtgtggc gttgcgctgc ttccagccaa
     5161 atttatccgc tccggtgcgc aagcactcga taagctgacg gcgagagaag cagcgcgtcg
     5221 ggtcggcggg gtcaacctga gtgtcattca ggatgcgaaa ctcgacggga tcgatgcccg
     5281 ctttttccgc cagttcgtcg atcgcgattt cgagcgccat cagaccgggg gcttcgccgg
     5341 gcgcacgcat ggcgttccct tccggcaaat caagcgtggc gagccgcagg ccggtatgac
     5401 gattcgcccc ggcgtagagt aattcgcttt gctgtaccgc cgtttccggc gtgccgccgg
     5461 gcaggtttcc agaccagctt tcatgtgaga tagcggtgat tttcccgctc tggtcggcac
     5521 cgatacgcaa gtgctgaagg gtggcg
//
LOCUS       DAZFVO020000237         5518 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFVO020000237 DAZFVO020000000
VERSION     DAZFVO020000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.239"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..4092
                     /gene="espI"
                     /locus_tag="ACHKNB_RS23665"
                     /old_locus_tag="pgaptmp_004733"
     CDS             1..4092
                     /gene="espI"
                     /locus_tag="ACHKNB_RS23665"
                     /old_locus_tag="pgaptmp_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HGU4198105.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFS
                     SLSRDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL
                     VTEVIPADIQGKDKFNNNRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTV
                     GKPYYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVT
                     LHNYGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQG
                     EKIFSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTW
                     TGAGIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQP
                     DANQKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAA
                     DYGAEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYY
                     LLKSNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNT
                     SVEIPKLAGNDILTLDGSVSISGDMSKQDGALIFQGHPVIHAGQTVSASQSDWENREF
                     SLNNLNLNNADFSLSRNAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKS
                     AAAGTSSYTGHITLEQKSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLN
                     IHKGGALTAQGGLFTSGSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSS
                     VLKARDQASVVGDIISDKQATISFGTESGKEGMLSEKASRGLAVGLLSGFNTAYRGAI
                     HAPSASATMNNTWWQLTGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAY
                     AMRTDLKNADKLVVNQKLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSE
                     QAIGFSNVTPVITAKDAGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNL
                     NKRMGDLRDINGEAGAWARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFT
                     VTHTDSSASADAFKGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGI
                     RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDRDY
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSR
                     MLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            4584..4949
                     /locus_tag="ACHKNB_RS23670"
                     /old_locus_tag="pgaptmp_004734"
     CDS             4584..4949
                     /locus_tag="ACHKNB_RS23670"
                     /old_locus_tag="pgaptmp_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198106.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHIQLERTRYRENIHDAECIREI
                     HEAPLQAPGILEKYKRTKS"
     gene            4959..5150
                     /locus_tag="ACHKNB_RS23675"
                     /old_locus_tag="pgaptmp_004735"
     CDS             4959..5150
                     /locus_tag="ACHKNB_RS23675"
                     /old_locus_tag="pgaptmp_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198107.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            complement(5301..>5518)
                     /locus_tag="ACHKNB_RS23680"
                     /old_locus_tag="pgaptmp_004736"
     CDS             complement(5301..>5518)
                     /locus_tag="ACHKNB_RS23680"
                     /old_locus_tag="pgaptmp_004736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198108.1"
                     /translation="YPSLSLILRYIHCLAFQGAGSEEYFVCASLPDYPAHLFMHPDTF
                     NTEVDFSNCQVFVESQRGSMICMLPQY"
BASE COUNT         1589 a         1197 c         1360 g         1372 t
ORIGIN      
        1 atgaataaaa tatacgcgct taaatatagc tcccttactg gtgggcttat agctgtgtca
       61 gaattaagta agaaggtcac aggaaaaacc ggcagaagat taatgacggt ttccctggta
      121 ttatcagtga ctctttctgc tttaccgggt aaagcatcaa cggttagcgc agaaatacca
      181 tatcagactt ttcgtgactt tgctgaaaat aaaggtgtgt ttacccccgg agtgacagga
      241 attgaaataa acgacaacaa tggaaataaa gttggggttc ttgatgttcc catgcttgat
      301 ttttccagtc tttctcgtga tggtcatacc acattgattc atcctggtta tgttgtatcg
      361 gctaaacatg gtggtttaca aagtgtttca tcagcaactt ttggttatga ccagatatat
      421 aaaatagttg ataataacct tgctggtata gatttttctg ccccacgatt aaataagctt
      481 gttacagaag taattcccgc agatatacag ggaaaggata aatttaataa taaccggtat
      541 acggcttttt accgtgcggg cgttggctct caatatatcc gttatgcaaa tggcacagat
      601 aaactactgc aggcttacac tccagaaaag gcttatctga ccggcggaac agtggggaaa
      661 ccttattata ctcactataa cggtatgaag atgatttcag caaacccggg aaataccttt
      721 gataaaaacc agggacctct tgccagttat ggacagagtg gagacagtgg ttcgccgtta
      781 tatgcctggg ataacattga caaaaaatgg gtattagctg gagttactct gcataattat
      841 ggagtaaaag gtgcacgaaa tgactggctc ctgatacctc atgactttat cagtcaaaaa
      901 ttacaggatg accttaaacc aattattgtt gcttctccgg aggagaatat cttacgctgg
      961 gaatttgatc gttcgagagg tacaggtact ctcagtcagg gagagaagat tttttccatg
     1021 actggtagtg taaacggaaa tgcaaatacc gggaacaatc ttgtgttctc aggtaatgaa
     1081 gggaaaatcg aactggtatc cagtgtggag cagggagccg gatatcttca gtttgataaa
     1141 gactacactg tactgacaaa taataacagt acatggactg gtgccggaat tattgtcggt
     1201 gacgaggcaa atgtcaaatg gggagttaat ggcattgccg gtgataatct gcataaagtt
     1261 ggttccggga cattaactgt taatggtcat ggtgagaata aaggtggcct taaagttggg
     1321 gacggcgttg ttgttcttga gcaacagcca gatgcaaacc agaaacaaca ggcgtttagc
     1381 catatcaata tagccagtgg tcgggcaaca gttaaactta acggggcaaa ccaggtagat
     1441 gcagataata tcagctgggg atatcggggt gggaaactgg atttaaatgg gtatgatttt
     1501 accttttccc gtcttcaggc tgcagattat ggtgctgaaa tcagcaacga taatcagaca
     1561 gataaatcca tagtcacact ttcgttatct cctctgaaag cagaagaaat aaatgtggtt
     1621 gttaataata taaatataat gggggggaca ggcaaaccag gtgatctgta ttatacgacc
     1681 tttgacggaa attattatct gttgaaaagt aaccgatatg gcagcgcttt gtttggcgcg
     1741 ctgaataatc agagcgaatg gcaaaggctg ggtaaggata aagaaaaagc aattgggtta
     1801 tatactcaga tgaaaatgca ggaaagcgct cctttatcat atatatatca tggaaaaata
     1861 accggtaata ccagtgtgga aatccccaaa ctggcaggca atgatatttt aacgcttgat
     1921 ggctctgtca gtatatcagg agatatgtca aaacaggacg gtgctcttat cttccaggga
     1981 cacccggtta ttcatgcagg gcaaactgtt tctgcatcgc agagtgactg ggagaacagg
     2041 gagttctcac tcaacaatct gaatcttaat aatgcggact tcagtctgtc ccgtaatgca
     2101 tttatgaacg ggaatatcag ggccgttaac cagagcactg ttattatcgg cggagataca
     2161 gtctttactg ataaaaatga cggaacaggt aatgatgtca tcagtgttga agggaaatct
     2221 gctgccgcag gaacatcctc ctatacaggg catatcactc tggagcaaaa atcagcactg
     2281 gatatccgcg ataattttcg tggcggggtt acgtctgaag acagtcatat caatgtttct
     2341 tcatcttcag tcctgttctc agatgcatcg tcatttataa acagctccct gaatattcat
     2401 aaaggaggtg cgctgaccgc tcagggaggg ctgtttacaa gtggaagcat tgatattggt
     2461 gacgcttccc ttctgcttac cggtacacca gtgaattcag atgatgctgc ttttttaccg
     2521 accatcaata tggctgatgg cggatttaaa ctgatgtctg attcatcagt actgaaagcc
     2581 agagaccagg catctgttgt tggtgatatt atttctgata aacaggccac aatcagcttc
     2641 ggaactgaat caggtaaaga gggcatgttg tctgagaagg catcccgggg actcgcggta
     2701 ggattactga gtggttttaa tacggcatac cgcggtgcaa ttcatgcccc gtcagcatct
     2761 gccactatga acaacacctg gtggcaactg acaggagact cctcacttcg ctcgttaaaa
     2821 aataccggaa gcatgacata ttttacagga agtgcagcga ataaagcatt ccatacactg
     2881 acggttgatg agctgacgac gaatggcact gcgtatgcca tgcgtacgga cctgaaaaat
     2941 gcggataagc tggtagtaaa ccaaaagctg tcaggtaagg acaatattct gctggttgat
     3001 tttctgaaca aacccaccgg agaaaaactg gatattgaac tggtgagtgc accggggaac
     3061 agcagtaagg atgtttttaa aggaagtgaa caggcaatag gtttcagcaa tgtcacacct
     3121 gttattacag ctaaagacgc cggagataaa acaacatgga acctgaccgg gtacaggatg
     3181 gcagaaaatc ccgccgcaac ccaaagtgcc tcaggccttg catctgtggg gtacaaatca
     3241 tttttgagtg aggtcaacaa cctgaataaa cgtatgggtg acctgcgtga catcaatggt
     3301 gaagctggcg catgggcacg tatcatgagc ggaaccggct ctgccggtgg tggtttcagt
     3361 gacaaccaca cacatgttca ggtcggtgtc gacaaaaaac atgagctgga cggactggat
     3421 ttgtttaccg gcttcactgt cacacacact gacagcagtg cctctgctga tgctttcaaa
     3481 ggtaaaacaa aatctgtggg ggccggactc tatgcttccg ccatgtttga ttccggtgcc
     3541 tatatcgacc tgattggtaa gtatgttcat catgataatg agtacaccgc aacctttgcc
     3601 ggactcggaa tccgtgatta cagtacgcat tcatggtatg ccggtgctga agcaggctac
     3661 cgctgtcatg tcactgagga tacctggatt gagccacagg cagaactggt ttacggtgct
     3721 gtatccggta aacagtttgc atggaaggac caggggatgc atctgtctat gaaggacagg
     3781 gactacaatc cgctgattgg tcgtaccggt gtggatgtgg gtaaatcctt ctcaggtaag
     3841 gactggaaag tgacagcccg tgccggtctg ggctaccagt tcgacctgct ggctaacggc
     3901 gaaaccgtat tgcgggacgc atcaggtgaa aaacgtatca aaggtgaaaa agacagccgt
     3961 atgctgatgt ccgttggcct gaatgcagaa atcagggaca acgtccgctt tggactggag
     4021 tttgagaaat ccgcctttgg taagtacaac gttgataatg ctgtcaacgc taacttccgt
     4081 tactcgttct gatactccgt attcaaaagg gcctgcgggc cctttatgct caggacctgc
     4141 cgcccgggta tacatttccg gggtatcagt cgatttgttt acttaaccgc catgaccaaa
     4201 gccggcagca atgtcggctt tttacgtcca tatattccac tcatccggaa ctgtgacaca
     4261 gaacctgaat acaggattga tcaccggtgc ccacaaatgg cacattttca gtaagattct
     4321 gaatgttaca ttcccccgtc atgtgtggca caactgtcac cgggaaagaa acggaaagca
     4381 catgaaaaac ataaaaactc acaccggact ccttatccac aaagaacaag cgcggcgcgt
     4441 tcgtcttcat gaaactccgg ccgcctggtg ccacacgcac cgggaatgct acagcaaaac
     4501 gaccggtcgc cgctgcggca gccctgacag tctcagcagg ctgattctga gcagcattca
     4561 gcccatagaa tgagatgacc cgtatgaatg atggcagaca gacgcctccg tcccctgagg
     4621 catggaaaat ccggtttctg gcgaacggta cacccggcac atggttttac accgatgacg
     4681 cagccgtcac aaaagagtgg ctgcgggcac attgtgacga tgtggaagtg atccccctgt
     4741 gccgtatgca tgacggtccc gttttccggg aacaccatat taatgaactc agcgccgcac
     4801 tggccagaat gctccatatg caccacatac agctggaacg aacccggtac agagaaaata
     4861 tccatgatgc agaatgtatt cgtgaaattc acgaagctcc cctgcaggct cccggcattc
     4921 tggaaaaata caaacgcaca aaatcataag gcagttctat gtggatactg attttctggc
     4981 tctcatcccc ctttaatgtc tcctccggcg atgcccggca acccacactg gccgttcaca
     5041 gtcaggagtt ccgtactgaa aaagcctgtc gggatgcctt cagcgcgctg aaaaaagaaa
     5101 acgtgggaga attcgctctc cggggcgtct gcgtccccgg cggagaataa accggagatg
     5161 atgattctcc acaggagtat tcaatggcat accattcccc ggatgtcaca ctacattatc
     5221 cgtatctgcg ccggatgtcc tgggcgcaga taacggcagc agctgaagat gcggaaatac
     5281 atcatgatta ttcccgggca ttaatattgt ggcagcatgc atatcatgct gccacgctga
     5341 ctctcaacaa aaacctggca gttgctaaaa tcgacttctg tattaaacgt atccggatgc
     5401 atgaacaaat gagcaggata atccggaaga ctggcacaga cgaaatactc ttctgaaccg
     5461 gccccttgaa acgccagaca gtggatgtat cttaaaataa gagataggct tggatata
//
LOCUS       DAZFVO020000238         5492 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFVO020000238 DAZFVO020000000
VERSION     DAZFVO020000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.240"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..319)
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS23685"
                     /old_locus_tag="pgaptmp_004737"
     CDS             complement(<1..319)
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS23685"
                     /old_locus_tag="pgaptmp_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4198109.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLT"
     gene            791..1225
                     /locus_tag="ACHKNB_RS23690"
                     /old_locus_tag="pgaptmp_004738"
     CDS             791..1225
                     /locus_tag="ACHKNB_RS23690"
                     /old_locus_tag="pgaptmp_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198110.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGIVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            1669..2559
                     /locus_tag="ACHKNB_RS23695"
                     /old_locus_tag="pgaptmp_004739"
     CDS             1669..2559
                     /locus_tag="ACHKNB_RS23695"
                     /old_locus_tag="pgaptmp_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HGU4198111.1"
                     /translation="MEDALYAFNYTQNRDKLFANLISIIDGIIADGVVREEEVLYLDT
                     WLLEAKQIINNGVIKSLSARVSDILADGIITSEERDDLKNSLLQIQREILDIPEIDFY
                     SKDVDVHLLNGLCKGLIADRNLTQEEIRYLNWWLEQNGALKNNYPGKKLYALVKEILK
                     DGVITEDESLTLHKALVDFTGCDLESGVVDGLATRLPIDVGASIELEGKTYCLTGTFV
                     AGKRAVVENLIKNAGGNISSGITQKLDFLVIGTLSSRDWKFSSHGRKIEKAISYRDDN
                     GAKLKIISEEMLFDALPSSR"
     gene            complement(2542..3228)
                     /locus_tag="ACHKNB_RS23700"
                     /old_locus_tag="pgaptmp_004740"
     CDS             complement(2542..3228)
                     /locus_tag="ACHKNB_RS23700"
                     /old_locus_tag="pgaptmp_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4198112.1"
                     /translation="MKTLAERLKIGREKAGMSQAQLAEKIGLSQQSVAKIENGETLQP
                     RKIKEIAKVLGVSQKWLQLGIEDNASIPDLVVKEAESTALDPDIFVNIPVLDVELSAG
                     NGCLAEIVESAIDWFPLRRADLRKSGVCASNAKIVQIWGNSLLPVLNNGDLVAVDISQ
                     TVPIRDGDLYAVRDGVLLRVKILINLPDGGLILRSFNKDEYPDEILTFEDRRARIHVI
                     GRVFWSSRTW"
     gene            3364..3612
                     /locus_tag="ACHKNB_RS23705"
                     /old_locus_tag="pgaptmp_004741"
     CDS             3364..3612
                     /locus_tag="ACHKNB_RS23705"
                     /old_locus_tag="pgaptmp_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198113.1"
                     /translation="MTPEQLALSEAIALAGGQSELARKLTASSGHLVKQQHVWNWLNR
                     EKRPPAKLSIFIEKTTGISKEKLRPDIFQKIKDSSDEK"
     gene            3635..4192
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS23710"
                     /old_locus_tag="pgaptmp_004742"
     CDS             3635..4192
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS23710"
                     /old_locus_tag="pgaptmp_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4198114.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTTINDELYLSISKLQEHAALVYKIFCAPE
                     NSNARECAAPGVVASIASGCGETNA"
     gene            4338..4517
                     /locus_tag="ACHKNB_RS23715"
                     /old_locus_tag="pgaptmp_004743"
     CDS             4338..4517
                     /locus_tag="ACHKNB_RS23715"
                     /old_locus_tag="pgaptmp_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HGU4198115.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            4447..5337
                     /locus_tag="ACHKNB_RS23720"
                     /old_locus_tag="pgaptmp_004744"
     CDS             4447..5337
                     /locus_tag="ACHKNB_RS23720"
                     /old_locus_tag="pgaptmp_004744"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4198116.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTAQAPYKAGAGGR
                     SPELSTAIYDAPASFLSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSS
                     GGGSFPQSTEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLE
                     CSASFTSRNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSNPSVLSDVAHNASVLYSYISSIHQVWLQQLYPMLAKAESPLAVSLYDRINDA
                     ALLASLINLSLNPSEVRGRK"
BASE COUNT         1465 a         1200 c         1268 g         1559 t
ORIGIN      
        1 gagtcagtaa ttgtccctgg tcttcctgca gaatagtcag gcgaccaccg cgattgacat
       61 acatcggcgt ttcggtggtg tcttcttcgg aaattttccc gcggttagtc ggacgaacat
      121 atgagagttt gtgttttatt ttcacacggt tctcatcaaa cggttcgatt tccaggttga
      181 gcgagacctt ccctttggtt ttcgtgttca tcacacctga agcgacttca ctgagaacag
      241 cgccgatttt ggtttcaaat acgccgccgt ccagctcccc gataaatgcc tgcacatcag
      301 tactgcgttc gctagccatt ttgctgctcc tcatcatatc gaccctgcaa ggtcggttgg
      361 tttctccaca aaacagagaa gaacacctgc ggtggcagcc gcccgggtgg attgggttat
      421 gagcccgtcg tccggtgatg ctcttctctg ttttgtaaaa agagcggtac cagccggaag
      481 caagtgtaca aactggtacc gccaaagcag tggctgttgt ggtgaccggt gctgatctcc
      541 ggcttgcggt tatttcagac tctcacgggc gtttaattgc cccgccgaac agctcttttc
      601 cgcaatagct gcaatgtctt tcgcgcatca gcctgcgcat tcaccacaac tctaaggatt
      661 ctctctggtt gaaaatactt agctgttatg tgcctgcttt tagccacatc aggcgaggtg
      721 gacctggtta ttccccaaca acaaggattc ggttaatctg gttatcccca acaacgcaaa
      781 aggaaaagaa atgtccggta atatctacac gttgtacaaa tcccactgtg aaaatgttgg
      841 aaagtatcgg ggcattgaaa tcagtgggat agtgtcatca gtcgaaataa gcaaagtgga
      901 atcaagggca acattactta ctcttctgga tcttgtctta catgagcacc ggaagaaatt
      961 cggcactccc tataatcagt tgaatgggaa aaaggctctg gttcacctta ttctgatgaa
     1021 gcatcactgg atgccaaaac agattaatga gatgaaattt gatgaacttc ttctttcaat
     1081 tcaggatgaa ctcacacttg ataaaataag cgtaaccgcc cagaaatttt tagattatcg
     1141 agactggaga tcacaaattc atcactttga tgattttgac gaaaatgaat gggatcctaa
     1201 tttgtctgca caatatctaa agtaacatcc tgtgataaaa ccgtgatttc ctgatccagt
     1261 ttttttaagg agtctattgt ttcctgtcga taagacagca cttcacgaag ctggtttata
     1321 gctgccagct tctttgtcat ccactcataa atttactcat ctgtgtagct aggcgcgacg
     1381 attttgggtt ctgttttgtg catttcacac ctcctcaagt tatcagttac ttgttgatgg
     1441 ggaccagatt gttaaagagc taagcgtcct gtagggcgct tttttgttgc taacgaatca
     1501 tcctggactt catatgcccc aggcggctac ttcgtgggtg tcctgcctgt tcgttgtctt
     1561 aacaccttta agttgtaatt tagttgtggt tttgaatgtt gtcaacaact ttatgtggtt
     1621 tgaacgagta gccaaggagt gcaaggatta tcaaaaaaag gaggttgtat ggaagacgcg
     1681 ctttacgctt ttaattacac acagaaccgg gacaagttat ttgctaactt gattagcatc
     1741 attgatggaa tcattgcaga tggagttgtc cgtgaagagg aggttcttta cttagataca
     1801 tggttacttg aagcaaagca gattatcaat aatggagtta taaaaagtct atctgctcgg
     1861 gtgtcggata ttcttgcgga tggaataatc acatcagaag aacgtgatga ccttaaaaat
     1921 agccttctcc aaatacagag ggaaattctt gatatccctg aaattgattt ttactccaag
     1981 gatgtagatg tccatttact taatggacta tgtaaaggat taattgctga tcggaactta
     2041 actcaagaag aaataagata tcttaattgg tggcttgagc aaaatggagc tttgaagaac
     2101 aactacccag gaaaaaaact ttatgcactt gtaaaggaaa ttcttaaaga tggtgttatt
     2161 actgaagatg agagtttaac tctacataag gcattagtag acttcacagg atgtgacttg
     2221 gaaagtgggg tggtggatgg tttggcgacc aggctgccta ttgatgtagg ggcttcgata
     2281 gagttagagg gtaaaaccta ttgtcttaca ggcacttttg ttgcaggaaa gagagccgta
     2341 gttgaaaatt tgattaaaaa tgctggtggg aacatcagta gtggaattac tcaaaagttg
     2401 gattttttag taattgggac gctttcctcc cgtgattgga aattctctag tcacggaagg
     2461 aagatcgaaa aggctatatc ttatagggat gataatggtg caaaacttaa aattatttct
     2521 gaagaaatgc ttttcgatgc attaccaagt tcgcgatgac cagaataccc tacctataac
     2581 atgaattctg gctcgtctat cttcaaaggt gagtatttca tctgggtact catctttgtt
     2641 gaagcttcta agaatcaagc caccgtcagg taagttgata agtattttaa cccttagcaa
     2701 tacaccatct cgtacggcat aaagatcgcc atcacgaata ggaacggttt gagaaatatc
     2761 aacggcaaca agatctccat tattgagaac cggtaataaa ctgttccccc atatttgtac
     2821 gatcttggca ttagatgcac atacgccaga ttttctcaaa tctgctcttc ttaacggaaa
     2881 ccagtcaata gctgattcaa ctatttcagc cagacatccg ttacctgccg ataactcgac
     2941 atctaaaaca ggaatgttta cgaaaatatc ggggtctaat gcggtgcttt ctgcttcttt
     3001 tacaacaaga tcaggtatgg atgcgttgtc ttcaatacca agttgtaacc acttttgtga
     3061 tacacctaaa acttttgcaa tttctttaat tttgcgcggt tgtagagttt cgccattctc
     3121 tattttggct acagattgtt gtgaaagtcc aattttttca gctagttgag cttggctcat
     3181 gccagctttc tctctaccta tctttaatcg ttctgccagt gttttcacaa catatccctc
     3241 tcttttttga tgaggttaca actttatgtt ttagctttcc aacatctaaa agttgtggta
     3301 aaagttgtta atgttgtatt cttgcagctc gtaacaactt aactaccaaa aaaggagaaa
     3361 gctatgacac ctgagcaatt agccttatcg gaggcaatcg ctctggctgg tggtcaatca
     3421 gaattggctc ggaagctcac agccagcagc ggtcatttag taaagcaaca acatgtctgg
     3481 aactggttga acagagaaaa gcgtccccct gcaaagcttt cgatattcat tgaaaagacc
     3541 actggcatat caaaagaaaa attacgtcca gatatttttc aaaagattaa agattcatca
     3601 gatgaaaagt aaccacagtt ttaaggagat agccgtgggt aagcatcact ggaaaataga
     3661 aaaacagcct gagtggtacg tgaaagctgt cagaaaaact atcgcggcgt tgccgggggg
     3721 ttacgctgaa gccgctgact ggctggatgt aacagagaac gctttattca atcgccttcg
     3781 tgccgatggc gatcagattt tcccgctggg atgggcaatg attttacaac gtgctggtgg
     3841 aactcacttc attgctgacg ctgtggcgca gtctgcaaat ggcgtctttg tgtctcttcc
     3901 tgacgtcgag gatgtggaca acgccgatat taaccagcgt ctgctggaag tcattgaaca
     3961 gatcggcagt tattccagac agattcgttc agcaatcgaa gacggtgtag tggaaccgca
     4021 tgagaagaca acaattaacg acgagctgta cctctcaatt tcgaagctcc aggagcatgc
     4081 agcactggtc tacaaaatct tctgcgctcc agaaaatagt aacgcccgcg agtgtgcagc
     4141 tccgggcgtc gtggcgtcga ttgcttctgg ttgtggagaa actaacgcat gaatagttta
     4201 acggcaaata accgtttgtc gcaacagctg gtggtcagcg tcgctgaaca cctgttgtta
     4261 cggcatgaat gcagattacc aaatcacctg gctgtaagta accacagaga actttacctg
     4321 actgtggggg gcgagttgtg caggaactta accgctggtt tcgtgacgga agagggcttt
     4381 atgtccatgt tattcgttgg gagccagaaa cacagcgcgt tatctatctt cgcaaagact
     4441 acccgcatga gtgctttagt cctttgtgga aattcaggcg tgattttgtt gagtgtgaag
     4501 gaccaccagc atattgattc tgcaattccg ggacgttaca ctgctcaggc accttataaa
     4561 gcgggtgccg ggggtcgcag cccggaattg tcaacggcga tatatgacgc gccagcgtct
     4621 tttttatcgt ccgcgctcac gcacgccaga attatggtgg gctgggcagg ggagccgaaa
     4681 ggctcgccgg tctccgttga cgccggtact gcgaaccctg ttcagtctgc caccagtgag
     4741 tttcgcagct ccggtggtgg aagttttcca cagtcaacgg aggctgccat catggctacg
     4801 atcccaaccc tcactcaacc tgaaattgcc atcgttgatg gtcaggctgt tacttcatcc
     4861 ctggctgttg ccaacttctt ctccaaacgt catgacgatg tactgaaaaa gatccgcacg
     4921 cttgaatgct ctgcatcatt cacttcccgc aatttttcgg tgagtgatta caccgattgc
     4981 acaggccgca aactaccttg ctatcaaata acccgcgacg gctttgcgtt tcttgctatg
     5041 ggtttcacgg gtaaacgtgc tgcccagttc aaagaggcat acatcaatgc ctttaaccag
     5101 atggagaaac agctttcaaa tccctctgta ctgagcgacg ttgcacataa cgccagcgtt
     5161 ctctattcct acatttcatc aattcatcag gtctggctgc agcagcttta tcccatgttg
     5221 gcaaaagccg aatccccgct ggctgtaagt ctgtatgacc gcatcaacga cgcggcgcta
     5281 ctggccagtc tcataaattt gtcgctgaac ccttcagagg taagggggcg caaatgatcc
     5341 ggaatatttt caaacggttt accaatcaga ctttccgttg tcctcgtcct ggtcagtggt
     5401 acaccacgcc tgcagggcat gttctacgtg tcagcctggt tgaccgtgaa tgtcagaagg
     5461 tgatttgtga accgctgggc cgtaattacc gc
//
LOCUS       DAZFVO020000239         5382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFVO020000239 DAZFVO020000000
VERSION     DAZFVO020000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.241"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(35..448)
                     /gene="hiuH"
                     /locus_tag="ACHKNB_RS23725"
                     /old_locus_tag="pgaptmp_004745"
     CDS             complement(35..448)
                     /gene="hiuH"
                     /locus_tag="ACHKNB_RS23725"
                     /old_locus_tag="pgaptmp_004745"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HGU4198117.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            581..1252
                     /gene="hprR"
                     /locus_tag="ACHKNB_RS23730"
                     /old_locus_tag="pgaptmp_004746"
     CDS             581..1252
                     /gene="hprR"
                     /locus_tag="ACHKNB_RS23730"
                     /old_locus_tag="pgaptmp_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HGU4198118.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1252..2610
                     /gene="hprS"
                     /locus_tag="ACHKNB_RS23735"
                     /old_locus_tag="pgaptmp_004747"
     CDS             1252..2610
                     /gene="hprS"
                     /locus_tag="ACHKNB_RS23735"
                     /old_locus_tag="pgaptmp_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HGU4198119.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINNTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALIKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(2718..3569)
                     /gene="hchA"
                     /locus_tag="ACHKNB_RS23740"
                     /old_locus_tag="pgaptmp_004748"
     CDS             complement(2718..3569)
                     /gene="hchA"
                     /locus_tag="ACHKNB_RS23740"
                     /old_locus_tag="pgaptmp_004748"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HGU4198120.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYVD"
     gene            complement(4161..5324)
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS23745"
                     /old_locus_tag="pgaptmp_004749"
     CDS             complement(4161..5324)
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS23745"
                     /old_locus_tag="pgaptmp_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4198121.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         1516 a         1076 c         1226 g         1564 t
ORIGIN      
        1 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
       61 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
      121 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
      181 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gccttaattc
      241 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttgtcc gctttctttt
      301 caagagtgac tgtcacgtcg gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc
      361 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gccgttacta
      421 cggagagtac taaataacgc tttaacatgt ttatatcctt gtcatgtgaa tgagttttga
      481 ggatagcgaa aaagcggcca gggtaacctt acagcagcat tacaattttg taatgaacca
      541 ggctgtttct ataacatatg atttatggca tattattttc atgaagattt tacttattga
      601 agataatcaa aggacccagg agtgggtaac gcaggggctt tccgaagcgg gttatgtcat
      661 tgatgccgtt tctgatggca gagatgggct ttatcttgcg ctgaaggatg attatgcatt
      721 gatcattctg gatattatgc ttccgggtat ggatggctgg cagatcttac aaacgttaag
      781 aacagcaaag caaacccctg ttatttgcct tactgcaagg gattctgtcg atgacagagt
      841 cagagggctg gacagtggag caaatgatta tctggtaaaa cctttttcat tttctgagtt
      901 gctggcaagg gttcgggcac aattaaggca acatcacgct ttgaattcaa cattagaaat
      961 cagtggctta agaatggact ctgtaagtca aagtgtgagc agggacaata tcagtattac
     1021 actgacgcgc aaggagtttc agttactttg gctactggcc tccagagctg gcgaaattat
     1081 acccagaacg gttattgcga gtgaaatttg gggaatcaac tttgatagtg ataccaatac
     1141 ggtggatgtc gccattcgca ggctccgcgc aaaagttgat gatccttttc ctgaaaagct
     1201 aatcgccaca atccggggga tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga
     1261 ttatctataa ccgtccgttt aaccttgctt tttatattgc tgctgtctgt tgctggcgct
     1321 ggaattgtct ggactctcta taatggcctg gcaagtgagt tgaaatggcg cgatgataca
     1381 acactcatta accggacagc acagatcaag cagttgttaa ttgatggggt aaatccagat
     1441 acgttacctg tgtactttaa ccggatgatg gatgttagtc aggatatctt gatcattcat
     1501 ggtgatggca tcaataaaat tgttaaccgg acaaatgtca gtgatggcat gttaaataac
     1561 atacctgcta gtgagacaat cagcgcagct ggcatttaca gaagcattat taataacaca
     1621 gagatagatg ctttacgaat taatattgat gaagtttcgc catcattaac ggttactgtg
     1681 gctaaattgg cttcagccag acataacatg cttgaacagt ataaaattaa tagcattata
     1741 atttgcattg tcgccattgt actttgctca gtattaagtc cgctgttaat cagaacgggg
     1801 ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc tgaattataa cgatagccgg
     1861 gagcctgttg aggttagcgc attaccgaga gaactaaaac ctcttgggca ggcgttgaat
     1921 aaaatgcatc aagccttaat caaagatttt gagcgactaa gtcagtttgc tgacgatctc
     1981 gctcatgaac ttagaacgcc aattaatgca ttactgggtc agaatcaggt tacgctcagt
     2041 caaaccagaa gtatcgctga atatcaaaaa acaattgccg gtaacattga agagctggaa
     2101 aatatttcgc ggttaacaga gaacatactg tttcttgcca gggcagataa aaacaatgtt
     2161 ttggtgaaac tggactcgct ttctctcaat aaggaagtcg aaaatttgtt ggactatctt
     2221 gaataccttt cagacgagaa agagatttgc tttaaggtcg agtgcaatca gcaaatcttt
     2281 gcggataaaa ttttactgca acgaatgtta tcgaatctta ttgttaacgc cattcgatat
     2341 tcgccagaaa aatcgcgcat tcatataacc agttttcttg ataccaacgg ctatcttaat
     2401 attgatgtcg ccagtcctgg aacgaaaatt catgagcctg aaaaactctt ccgtagattt
     2461 tggcggggag ataattcgcg tcattccgta ggtcagggac ttggtctttc tttagtcaaa
     2521 gcgattgccg aattgcatgg gggaagtgct acgtatcact atctcaataa gcataatgtg
     2581 ttccggatta tgttaccgca aagaaattaa catagggctt cagtacgcca taaatcaacc
     2641 atgaaaaaac gcagccacgt cgtgagtatt aacgtggcta cgattgatta tgcgtttaca
     2701 ttcaaacgta acagggatta atccacataa gctgccagca tttcctgcgc cgccagttta
     2761 cccaacgcat tcgctgcgaa aggactgtcg ccggtgagaa gtttacggtc cttatgtact
     2821 cgcccggtga tgtcgtcatt aatgattttc atgcccattt tcttcagctc ttcgccgaag
     2881 taccaggtga gatgacctgg catatagcca atatctggcg tttgtttgtc tgcggcgtct
     2941 gggaatgcgc aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa
     3001 gccgccgggc cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgtaaa
     3061 gcggcagcta cgtcctggct ttcaggtaaa ccaataagtg cgccatgacc accaggaaca
     3121 aagattgctg catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc
     3181 ggattgcgga acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc
     3241 ggcatagccc agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg
     3301 aaacctgcag catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc
     3361 gagaacagtt ttccgttatc ggttggcaaa taacgttcgt ctgcggcgat caccagaatt
     3421 ttatgtttac cgcgatacgg ttttggatag tccacgccat caagatcaga gacaggactg
     3481 gtatattggc taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc
     3541 tgcggatttt tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt
     3601 agtcactata tttgcgactg agctggtcgc tatttttaac caggctggaa aattgagtca
     3661 atatatcgaa agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt
     3721 gaatattatt cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatta
     3781 ttttagacca ttatcagagg tacggggcga cggggagaga tttagtgctg tggaatattg
     3841 tggtgctgat gaatatttat atcccggatt ggtagagaaa aagcttattt catgcactat
     3901 ttttgcaaat atttctatat gggaggcggt tttgttgcag attaattcat aaagaaatgc
     3961 atttgggtct tggctggaag agtcgctggt agatgttatt gtgccagcag gggcagggta
     4021 aggtgctgta tcgatatgca ttggcaattg cgtgtgccgc agatggagat gaaatgccaa
     4081 taaaaaagcc tgaattcgat gagggacgaa ctcaggctag tgtttagttt tacagtaaca
     4141 cgccgcagca cgttggctga ttagaactgg tagactaagc ccacaccaac aacgtcatct
     4201 gttgcgatgc cattgttgtc atagaaatcg tcatctttat ccaacaagtt tattttatag
     4261 tcaacatatg ttgagaagtt cttattgaaa tagtaggtcg ctccaatttc aacatagttg
     4321 accagatcct gatcaacata ctcttgatga ccattgttgt tatagtcgtt accgccgagg
     4381 tctttgcctt ttgaataaac atatgcaaga gatgggcgca gaccaaaatc gaactgatac
     4441 tgtgctacag cttcaaagtt ctgggtttta tttgcgatac ccacattacc atatggtgtc
     4501 atattgcggg tttcagcata catcattgca agataaacat tgttggcgtc gtattttgca
     4561 ccaaatgtcc atgcttcagc tgtatcacca ccagcaaatt tgctgtattc attgtagtga
     4621 tacccgccaa gagctacttg agaattggta cggtcagagt ttgagtaagc ggcacctaag
     4681 ctcaaaccga agtcaaagtc atatgaagtg gaaagaccaa aaccatcacc gttctggaag
     4741 cgaacgtctt cgtgaccatt tttagtacct tcttgatctt ctcctgcatt gctgccttcg
     4801 ttgttacctt gatactgcaa tgcaaagttc agaccttcca ccagaccgaa gaaatcacta
     4861 ttgcggtaag tagctacccc atttgcacgg cctgccatga agttatcagt ccaggtatag
     4921 gagtcgccgc cgaatacagg aagcatatct gtccaggctt caacgtcgta aatcacacca
     4981 tagttacgac catagtcaaa ggagcccatc tcgccgaact tcagacctgc aaaagcaagt
     5041 ttgacataag tattatcaga accttcagtg tcgctagcct ttgttttggt cagtgcttga
     5101 ccataaccag ttaactgatc tgtaatttgg gtctcacctt tgaagcctaa ctcgatgata
     5161 gtttcatcgc cgtcatcgcc agggttatcg gaaaaagtat gacgagcatc aacttttccg
     5221 tacagatcca gtttattgcc atttttatta taaatttcag ccgcatttgc tgcgccagca
     5281 actaataacg ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg
     5341 attataatct tcatgaatat atcaataagt gccgttatcc aa
//
LOCUS       DAZFVO020000240         5331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFVO020000240 DAZFVO020000000
VERSION     DAZFVO020000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.242"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..163
                     /gene="yhfL"
                     /locus_tag="ACHKNB_RS23750"
                     /old_locus_tag="pgaptmp_004750"
     CDS             <1..163
                     /gene="yhfL"
                     /locus_tag="ACHKNB_RS23750"
                     /old_locus_tag="pgaptmp_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HGU4198122.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
     gene            459..1796
                     /gene="frlA"
                     /locus_tag="ACHKNB_RS23755"
                     /old_locus_tag="pgaptmp_004751"
     CDS             459..1796
                     /gene="frlA"
                     /locus_tag="ACHKNB_RS23755"
                     /old_locus_tag="pgaptmp_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HGU4198123.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1817..2839
                     /gene="frlB"
                     /locus_tag="ACHKNB_RS23760"
                     /old_locus_tag="pgaptmp_004752"
     CDS             1817..2839
                     /gene="frlB"
                     /locus_tag="ACHKNB_RS23760"
                     /old_locus_tag="pgaptmp_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HGU4198124.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2889..3770
                     /gene="frlC"
                     /locus_tag="ACHKNB_RS23765"
                     /old_locus_tag="pgaptmp_004753"
     CDS             2889..3770
                     /gene="frlC"
                     /locus_tag="ACHKNB_RS23765"
                     /old_locus_tag="pgaptmp_004753"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HGU4198125.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYACQALERFRALLPEDER
                     WKPWRQSAITASISTRN"
     gene            3716..4501
                     /gene="frlD"
                     /locus_tag="ACHKNB_RS23770"
                     /old_locus_tag="pgaptmp_004754"
     CDS             3716..4501
                     /gene="frlD"
                     /locus_tag="ACHKNB_RS23770"
                     /old_locus_tag="pgaptmp_004754"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HGU4198126.1"
                     /translation="METLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4601..>5331
                     /gene="frlR"
                     /locus_tag="ACHKNB_RS23775"
                     /old_locus_tag="pgaptmp_004755"
     CDS             4601..>5331
                     /gene="frlR"
                     /locus_tag="ACHKNB_RS23775"
                     /old_locus_tag="pgaptmp_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4198127.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         1207 a         1428 c         1417 g         1279 t
ORIGIN      
        1 caaatttatt aaagttgcac tggtaggtgc agtactggct acgttaactg catgtactgg
       61 tcatattgaa aaccgtgata agaactgctc ttacgactac ctgctgcacc cggcaatttc
      121 tatttctaaa atcattggcg gttgcggtcc tactgcacag taagtctctg acagaaatcg
      181 gctaacaccg gtttaattgc ccgacgccct ctggattttc cgagggcgta tttgtttcta
      241 tatccttctt gctccccacc cgtaaaaata ttttcacttt aatttcaatt tgataaccac
      301 attcattagc cacagcgcga tcctatgcga aattttgtga tcttcctccg cattacataa
      361 catcatatgt tgttatattc attatgcatt gtcatgttac cttttaaatg actgcaaact
      421 ctcccctaca acgtgttgga caagaaaaag gtatcgctat gggaagccag gaactccaac
      481 gcaagctcgg attttgggcc gttcttgcaa tcgccgtcgg gacaaccgtc ggctccggta
      541 tttttgtatc tgtgggtgaa gtggcaaaag cggcgggcac gccgtggctg acggtgctcg
      601 catttgtcat cggcgggtta attgtgatcc cgcaaatgtg cgtctatgcg gaactatcaa
      661 ccgcttatcc ggaaaatggc gcagattatg tttatctgaa aaatgccgga agccgaccgc
      721 tggctttcct ctccggctgg gccagcttct gggccaacga tgcgccgtca ttgtcgatta
      781 tggcgctggc gattgtcagc aatcttggct ttttaacgcc tatcgatccg ttgcccggta
      841 aatttatcgc cgccggatta attatcgcct ttatgttgct acacctgcgc tccgttgaag
      901 gcggcgcagc gtttcagacg ctaattacca tcgccaaaat tatcccgttc actatcgtca
      961 ttggccttgg gatcttctgg tttaaagcgg agaattttgc cgcccctacc accactgcga
     1021 ttggcgcaac gggcagcttt atggcgctgc tggcggggat ctctgccacc agttggtcgt
     1081 ataccggcat ggcctctatc tgttatatga ccggcgaaat taaaaacccc ggaaaaacca
     1141 tgccacgagc gctgattggt tcctgtctgc tggttctggt gctctacacc ctgctggcgc
     1201 tggtgatttc cggcctgatg cccttcgaca aactcgccaa ttctgaaacg ccgatttccg
     1261 acgccctgac ctggatcccc gcactcggca gcaccgctgg gatctttgtt gccatcacgg
     1321 cgatgatcgt cattcttggt tcgctttcca gctgcgtgat gtaccagccg cggctggaat
     1381 acgcgatggc gaaagacaac ctgttcttta aatgcttcgg ccatgtgcat ccgaaataca
     1441 acacgccgga tgtctccatc atcctgcaag gggcgctggg gatcttcttc atcttcgttt
     1501 ccgatctcac cagcctgctg ggttatttca ccctggtgat gtgtttcaaa aataccctca
     1561 ccttcggctc catcatctgg tgtcgtaaac gcgacgatta caaaccgctg tggcgtactc
     1621 cggctttcgg gctgatgacc accctcgcca ttgcgtcaag cctcattctg gtcgcctcaa
     1681 cctttgtctg ggcaccgatt cccggcctta tctgcgccgt catcgttatt gctactggtc
     1741 tgcctgctta cgccttctgg gcgaagcgta gccgccagct caacgctttg tcgtaatgtt
     1801 atctggagaa aataaaatgt tggatattga taaaagcacc gtggactttc tggtcaccga
     1861 aaatatggtt caggaagtgg aaaaagttct cagccatgac gttccgctgg tgcacgccat
     1921 cgtggaagag atggtgaagc gcgacattga tcgtatttat ttcgttgcct gcggctcgcc
     1981 actcaacgcg gcgcaaacgg cgaaacatct ggcggatcgc ttttccgatc ttcaggtcta
     2041 cgccatttcc ggctgggagt tctgcgataa caccccgtat cgcctcgacg atcgttgcgc
     2101 ggtgattggc gtttctgact acggtaaaac ggaagaggta atcaaagcgc tggagctggg
     2161 ccgggcctgc ggcgcactca ctgcggcgtt caccaaacgc gcggacagcc cgattacctc
     2221 ggcggcggaa tttagcattg attatcaggc cgactgtatc tgggaaattc acctgctgct
     2281 ctgctacagc gtggtgctgg agatgatcac ccgcctcgcg ccgaacgcgg aaatcggcaa
     2341 gatcaaaaac gatctcaagc agttgccgaa tgcgctcggt catctggtac gcacctggga
     2401 agaaaaaggc cgccagcttg gtgaactggc cagccagtgg ccgatgattt ataccgttgc
     2461 tgcgggtccg ctgcgtccgc tgggttacaa agaaggcatt gtaacgctga tggaatttac
     2521 ctggacgcac ggctgcgtga ttgagagcgg agagttccgc catggcccgc tggagattgt
     2581 cgaaccgggc gttccgttcc tgttcctgct cggcaatgat gaaagtcgcc acaccaccga
     2641 acgcgccatt aactttgtta aacagcgtac tgacaacgtg atcgtcatcg attacgccga
     2701 aatttcgcaa gggctgcacc cgtggctggc accgttcctg atgttcgtgc caatggagtg
     2761 gctctgctac tacctgtcta tttacaaaga tcacaacccg gatgaacgcc gctattacgg
     2821 tggtctggtg gaatattaat ccctctcccg gcccggcaac gggccgaatt tacgcaagga
     2881 gttacccgat gaaaacaggt atgtttacct gcggccacca gcggctgcct attgaacacg
     2941 catttcgtga tgcaagcgag ctgggttacg acggcatcga aatttggggc ggtcgcccgc
     3001 acgcgttcgc gccggactta aaagcgggcg gcatcaaaca aatcaaggcg ctggcgcaga
     3061 cgtatcagat gccgattatc ggctatacgc cagaaaccaa cggctatccg tataacatga
     3121 tgctgggcga tgaacatatg cgtcgcgaaa gcctcgacat gatcaagctg gcgatggata
     3181 tggcaaaaga gatgaacgcg ggttatacgc tgatttccgc ggcccacgcg ggctatctca
     3241 cgccacctaa tgttatctgg ggacggctgg cagagaacct aagcgaactg tgtgagtacg
     3301 cggaaaacat cggcatggat ctgatcctcg aaccgttaac gccgtatgaa tcgaacgtcg
     3361 tgtgtaatgc taatgatgtg cttcatgcgc tggcgctggt gccttcgccg cgcctgttca
     3421 gcatggtcga catttgcgcg ccgtatgtcc aggcggaacc ggtgatgagt tatttcgaca
     3481 aactgggtga taaattgcgt catctgcata ttgtcgacag cgacggggcc agtgacacac
     3541 attacattcc cggcgaaggc aaaatgccgc tgcgggagct gatgcgcgat attatcgacc
     3601 ggggctatga cggttactgc acggtggaac tggtgacgat gtatatgaac gagcccagac
     3661 tctatgcctg ccaggcgctg gaacgctttc gcgcgctgct gccggaggat gagagatgga
     3721 aaccctggcg acaatcggcg ataactgcgt cgatatctac ccgcaactga ataaagcgtt
     3781 ttctggcggt aatgcggtca atgtggcggt gtactgcact cgctacggca tacagccggg
     3841 atgcattacc tgggtgggtg acgatgacta cggcacaaag ctgaagcagg atctcgcccg
     3901 catgggcgtc gatatcagcc atgtccatac gaaacacggc gttaccgcac aaactcaggt
     3961 ggaactgcac gacaatgatc gcgtttttgg cgactacacc gaaggcgtga tggccgactt
     4021 tgccctgagt gaagaggatt acgcctggct ggcgcagtat gacattgtgc acgcagcaat
     4081 ctggggacat gcggaagacg cattcccaca gctgcacgct gcgggcaaac ttaccgcttt
     4141 cgacttctcc gacaagtggg acagcccgct ctggcagaca ctggtgccgc atctcgattt
     4201 tgcctttgcc tccgcaccgc aagaagacga aacgctgcgt ctgaagatga aagcgattgt
     4261 tgcccgtggc gcaggaacag tgattgtcac gctgggtgaa aacggcagca ttgcctggga
     4321 tggcgcgcag ttctggcgtc aggctcctga accggtgacg gttatcgaca ccatgggtgc
     4381 cggagattcg ttcattgccg gattcctttg cggctggtct gcggggatga cattaccgca
     4441 ggcgatggcg cagggaacgg cgtgcgcggc gaaaaccatt cagtaccacg gtgcctggta
     4501 ggtataacgt tggcgtgagc atcttcacgc caacgtgctg ttacttgccg gaaaacgacc
     4561 ctataatccg agtaattcat tctttatttc agggtcgatt atgtcagcta cggaccgcta
     4621 ctctcatcaa ctcctctacg ctaccgtccg ccagcgactg ctggatgata tcgcgcaggg
     4681 ggtttaccag gccgggcaac agatccctac cgaaaacgag ctttgtacac aatataacgt
     4741 cagccgcatt accattcgca aagccatcag cgacttagtg gcagacggcg tactgatccg
     4801 ctggcaggga aaaggcacct ttgtacaaag ccagaaagtt gaaaacgccc tgcttactgt
     4861 cagtggtttt accgattttg gcgtctcaca aggcaaggcg acgaaagaga aagtgatcga
     4921 acaggaacgg gtcagcgccg cgccgttttg cgaaaagctg aacatccccg gaaacagcga
     4981 agtgttccat ctctgccggg tgatgtatct cgataaagag ccgctgttta ttgatagttc
     5041 atggatcccg ctgtcgcgtt atcctgactt tgatgagatt tacgtcgaag gaagctccac
     5101 ctatcagtta tttcaggagc gttttgacac gcgagtggtc agcgacaaaa agaccatcga
     5161 tatctttgcc gccacccgcc cgcaggcaaa atggctgaaa tgcgaactgg gcgaaccgtt
     5221 gtttcgcatc agcaaaatcg cctttgatca gaatgacaaa ccggtgcacg tctccgaact
     5281 cttctgccgc gccaatcgca tcaccttaac tattgataat aaaagacatt a
//
LOCUS       DAZFVO020000241         5282 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFVO020000241 DAZFVO020000000
VERSION     DAZFVO020000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.243"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            192..1526
                     /gene="garP"
                     /locus_tag="ACHKNB_RS23780"
                     /old_locus_tag="pgaptmp_004756"
     CDS             192..1526
                     /gene="garP"
                     /locus_tag="ACHKNB_RS23780"
                     /old_locus_tag="pgaptmp_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HGU4198128.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1542..2312
                     /gene="garL"
                     /locus_tag="ACHKNB_RS23785"
                     /old_locus_tag="pgaptmp_004757"
     CDS             1542..2312
                     /gene="garL"
                     /locus_tag="ACHKNB_RS23785"
                     /old_locus_tag="pgaptmp_004757"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HGU4198129.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2342..3232
                     /gene="garR"
                     /locus_tag="ACHKNB_RS23790"
                     /old_locus_tag="pgaptmp_004758"
     CDS             2342..3232
                     /gene="garR"
                     /locus_tag="ACHKNB_RS23790"
                     /old_locus_tag="pgaptmp_004758"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HGU4198130.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3329..4474
                     /gene="garK"
                     /locus_tag="ACHKNB_RS23795"
                     /old_locus_tag="pgaptmp_004759"
     CDS             3329..4474
                     /gene="garK"
                     /locus_tag="ACHKNB_RS23795"
                     /old_locus_tag="pgaptmp_004759"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HGU4198131.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4507..4883
                     /gene="rnpB"
                     /locus_tag="ACHKNB_RS23800"
                     /old_locus_tag="pgaptmp_004760"
     ncRNA           4507..4883
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKNB_RS23800"
                     /old_locus_tag="pgaptmp_004760"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1258 a         1254 c         1487 g         1283 t
ORIGIN      
        1 caaccttatg tgcatatgct caaaataata tttaaatagc attaattaaa ttagcatttg
       61 cacaatgatt attattctcg cgctgcttat actaattccc gagttcattg ccgttacggc
      121 ataaatgaaa ctcataaata acaccttccc cataataata aaaatcagca taagtacccg
      181 aggtaaataa aatgattctg gacaccgttg acgaaaaaaa gaaaggcgtg catacccgct
      241 atttaatatt actgattatt tttattgtta ccgccgttaa ctacgccgat cgtgcaacgc
      301 tgtctattgc tggtaccgaa gtggcaaaag agttgcagtt aagtgcggtt tcgatgggtt
      361 acatcttttc tgcttttggc tgggcctact tgctgatgca aatccccggc ggctggctgc
      421 ttgataagtt tggctcgaaa aaagtttata cctatagcct ctttttctgg tcgctattca
      481 ctttcctgca aggctttgtt gatatgttcc cgctggcctg ggcagggatc tccatgttct
      541 ttatgcgctt tatgctcggc ttctcggaag cgccatcatt cccggcgaac gcccgtattg
      601 tcgccgcctg gttcccgacg aaagaacgtg gtactgcctc cgccatcttt aactcggcgc
      661 aatatttctc gctggcgctc ttttcgcctc tgcttggctg gctgactttc gcctggggct
      721 gggagcacgt ctttaccgtt atgggggtga ttggttttgt gctgacggcg ctgtggatca
      781 aattgattca taacccgaca gatcacccac gtatgtctgc ggaagagctg aagtttatct
      841 ctgaaaatgg cgcggtggtc gatatggacc acaaaaagcc gggcagtgcg gcagcaagcg
      901 gtcccaaact gcattacatc aagcaattgc tctctaaccg catgatgttg ggcgtatttt
      961 tcggacaata ttttatcaac accatcacct ggttcttcct cacctggttc ccgatttatc
     1021 tggtgcagga aaaaggcatg tcgattctga aagtgggtct ggtcgcctcg attccggcac
     1081 tgtgtggttt tgcgggcggc gtgctgggag gtgtcttctc ggattatctg atcaaacgcg
     1141 gtttatccct gaccctggca cgtaagctac cgattgtgct gggaatgttg ctggcttcca
     1201 ccatcatctt atgtaactac accaacaaca ccacgctggt ggtcatgcta atggcgctgg
     1261 ctttttttgg caaaggattt ggtgctctgg gctggccggt gatttctgac accgcgccga
     1321 aagagattgt tggcctctgt ggcggcgtct ttaacgtctt tggcaatgtt gcctccattg
     1381 tcactccact ggtgattggc tacctggtaa gtgaactgca ctccttcaat gcagcactgg
     1441 ttttcgtggg atgttcagcg ctgatggcga tggtctgcta cctcttcgta gttggcgaca
     1501 ttaaacgtat ggaattgcag aaataagcaa aggtataagc gatgaataac gatgttttcc
     1561 cgaataaatt caaagccgca ctggctgcga aacaggtaca aattggttgc tggtcagcac
     1621 tctctaaccc gattagcact gaagttcttg gtttggctgg gtttgactgg ctggtgctgg
     1681 atggcgaaca tgcgccaaac gatatctcca cgtttattcc gcagttaatg gccctgaaag
     1741 gcagcgccag cgcgccagta gtgcgagtgc cgaccaacga gccggtaatt attaagcgtc
     1801 ttctggatat cggtttctat aacttcctga ttccttttgt agaaacaaaa gaggaagcag
     1861 agcaggcggt ggcatcaacc cgttacccac cggaaggcat tcgcggcgtc tccgtttctc
     1921 accgcgccaa tatgtttggc accgtggcgg attatttcgc tcagtcgaac aagaacatca
     1981 ccattctggt ccagatagaa agtcagcagg gcgtagataa cgtcgatgcc attgccgcta
     2041 ccgaaggtgt agacggtatc ttcgtcggcc ccagcgatct ggctgcggca ttaggccatc
     2101 tcggcaatgc ctcgcacccg gatgtacaaa aagcaattca gcacattttt aaccgtgcca
     2161 gtgcgcacgg caaacccagc ggtattctcg cgccggtcga agccgatgcg cgtcgttatc
     2221 tggaatgggg cgcgacgttt gtggctgtcg gcagcgatct tggcgtcttc cgctctgcca
     2281 ctcagaaact ggctgatacc tttaaaaaat aaccaccacc gcaacaagag agatgattga
     2341 tatgactatg aaagttggtt ttattggcct ggggattatg ggtaaaccaa tgagtaaaaa
     2401 ccttctgaaa gcaggttact cgctggtggt tgctgaccgt aacccagaag ctattgctga
     2461 cgtgattgct gcaggtgcag aaacagcgtc tacggctaaa gcgatcgctg aacagtgcga
     2521 cgtcatcata accatgctgc caaactcccc tcatgtgaaa gaggtggcgc tgggtgagaa
     2581 tggcatcatt gaaggcgcga agccaggtac ggtattgatc gatatgagtt ctatcgcacc
     2641 gctggcaagc cgtgaaatca gcgaagcgct gaaagcgaaa ggcattgata tgctggatgc
     2701 tccggtgagc ggcggtgaac cgaaagccat cgacggtacg ctgtcagtga tggtgggcgg
     2761 cgacaaggct attttcgaca aatactatga tttgatgaaa gcgatggcgg gttccgtggt
     2821 gcataccggg gaaatcggtg caggtaacgt caccaaactg gcaaatcagg tcattgtggc
     2881 gctgaatatt gccgcgatgt cagaagcgct aacgctggca accaaagcgg gcgttaaccc
     2941 ggacctggtg tatcaggcaa ttcgcggtgg actggcgggc agtactgtgc tggatgccaa
     3001 agcgccgatg gtgatggacc gcaacttcaa gccgggcttc cgtattgatc tgcatattaa
     3061 ggatctggca aatgcgctgg atacttccca cggcgtcggc gcgcaactgc cgctcacagc
     3121 tgcggttatg gagatgatgc aggcactgcg agcagatggt ttaggaacgg cggatcatag
     3181 cgccctggcg tgctactacg aaaaactggc gaaagtcgaa gttactcgtt aatgacgtcg
     3241 tgcccggatg gcgtattgca atccgggcct ggaatccagg ccgaataaga gaaacgccct
     3301 ccggcgtcat gtggtaacag gcataggtat gaaaatcgta atcgccccag actcttataa
     3361 agaaagttta tctgccagcg aggttgcgca ggcgatagaa aaaggatttc gggaaatttt
     3421 tcctgatgca cagtacgttt ctattccggt tgccgacggt ggcgaaggaa cggtggaagc
     3481 gatgattgca gccacccagg gttctgaacg tcacgcctgg gttacagggc cgctgggcga
     3541 gaaagtgaat gccagttggg ggatctccgg cgatggcaaa accgcgttta ttgaaatggc
     3601 ggcggccagt gggctggagc tggtacctgc ggaaaaacgc gatccactcg tgaccacttc
     3661 acgcggcaca ggcgagttaa tcctgcaggc gctggagagc ggcgcgacaa acattattat
     3721 cggcattggc ggtagcgcta caaatgatgg cggcgcaggc atggtacagg cgctgggggc
     3781 gaaattatgc gacgccaacg gcaatgaaat tggttttggc ggcggtagtc ttaatactct
     3841 gaatgatatt gatatttccg gcctcgatcc gcgcttaaaa gattgcgtca ttcgcgtcgc
     3901 ttgtgatgtc accaatccgc tggtgggcga taacggcgca tcgcgcatct ttggcccaca
     3961 aaagggagcc agtgaagcga tgattgttga gctggacaat aacctctctc actatgccga
     4021 ggtcattaaa aaagcgctgc atgttgatgt gaaagatgtc cccggcgcag gagctgcggg
     4081 cggtatgggt gcggcgctga tggcgtttct tggtgcggaa ctgaaaagtg gtattgaaat
     4141 cgtcactacg gcgctgaatc tggaggaaca tattcacgat tgtacgctgg tgatcaccgg
     4201 tgaagggcgt attgacagcc agagtattca cggcaaggta ccgattggcg ttgcaaacgt
     4261 ggcgaagaag taccataaac cggtgattgg cattgcgggt agcctgaccg atgatgttgg
     4321 cgttgtacat cagcatggca tcgatgcggt cttcagcgta ttgaccagca taggtacgtt
     4381 ggacgaagca ttccgcgggg cttatgacaa tatctgccgt gcttcacgta atatcgccgc
     4441 gacactggcg attggaatgc gcaacgcggg gtgacaaggg cgcgcaaacc ctctatactg
     4501 cgcgccgaag ctgaccagac agtcgccgct tcgtcgtcgt cctcttcggg ggagacgggc
     4561 ggaggggagg aaagtccggg ctccataggg cagggtgcca ggtaacgcct gggggggaaa
     4621 cccacgacca gtgcaacaga gagcaaaccg ccgatggccc gcgcaagcgg gatcaggtaa
     4681 gggtgaaagg gtgcggtaag agcgcaccgc gcggctggta acagtccgtg gcacggtaaa
     4741 ctccacccgg agcaaggcca aataggggtt cataaggtac ggcccgtact gaacccgggt
     4801 aggctgcttg agccagtgag cgattgctgg cctagatgaa tgactgtcca cgacagaacc
     4861 cggcttatcg gtcagtttca cctgatttaa cgaaaacccg ccagcgtaaa ccggcgggtt
     4921 tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc caaaagaaag
     4981 cggcttatcg gtcagtttca cctgatgtac gtaataaacc gttccggcgg gtttcagatt
     5041 gttgagtgcg ctttattcat gccggatgcg gcgtgaacgc cttatcctgc ctacaaaagc
     5101 acgcaaattc aatatattgc agagatggcg taggcctgat aagcgtagcg catcaggcat
     5161 ttttgcggtt gccatcagtt tcaaacccgc gtcagtgagt ttttgctttt ggtgcacagg
     5221 gtaagatgaa taaatatcta cggtattaat aatagacttt gtaagaagtt attaagtcta
     5281 ta
//
LOCUS       DAZFVO020000242         5268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFVO020000242 DAZFVO020000000
VERSION     DAZFVO020000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.244"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..153
                     /locus_tag="ACHKNB_RS23805"
                     /old_locus_tag="pgaptmp_004761"
     CDS             <1..153
                     /locus_tag="ACHKNB_RS23805"
                     /old_locus_tag="pgaptmp_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198132.1"
                     /translation="ESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNG
                     GNYGLW"
     gene            140..1489
                     /locus_tag="ACHKNB_RS23810"
                     /old_locus_tag="pgaptmp_004762"
     CDS             140..1489
                     /locus_tag="ACHKNB_RS23810"
                     /old_locus_tag="pgaptmp_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HGU4198133.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGQVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1473..2597
                     /locus_tag="ACHKNB_RS23815"
                     /old_locus_tag="pgaptmp_004763"
     CDS             1473..2597
                     /locus_tag="ACHKNB_RS23815"
                     /old_locus_tag="pgaptmp_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4198134.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2587..3201
                     /locus_tag="ACHKNB_RS23820"
                     /old_locus_tag="pgaptmp_004764"
     CDS             2587..3201
                     /locus_tag="ACHKNB_RS23820"
                     /old_locus_tag="pgaptmp_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4198135.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3194..3631
                     /locus_tag="ACHKNB_RS23825"
                     /old_locus_tag="pgaptmp_004765"
     CDS             3194..3631
                     /locus_tag="ACHKNB_RS23825"
                     /old_locus_tag="pgaptmp_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4198136.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3631..4713
                     /locus_tag="ACHKNB_RS23830"
                     /old_locus_tag="pgaptmp_004766"
     CDS             3631..4713
                     /locus_tag="ACHKNB_RS23830"
                     /old_locus_tag="pgaptmp_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4198137.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            4704..5264
                     /locus_tag="ACHKNB_RS23835"
                     /old_locus_tag="pgaptmp_004767"
     CDS             4704..5264
                     /locus_tag="ACHKNB_RS23835"
                     /old_locus_tag="pgaptmp_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4198138.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
BASE COUNT         1140 a         1354 c         1626 g         1148 t
ORIGIN      
        1 gaaagcagtg gacaggcggt acaaaaagcc tctgcccgtg tggagctggt tgccccggaa
       61 gggtggcagg ctcgcagtat tgatattgat gacacgtccg gtcacggact ggatgtgaat
      121 gtctggaacg gagggaatta tggcctctgg tgacggtcgt ggtgcgttcc gtggtgtgcc
      181 gttcctcgtc tggcgtgagc agcgcgaacg tggtgggcga aacattgtcc gtcgggaata
      241 tccgttacgg gaaaccggtg gtgcagacga tctgggacct aaactggctg aatttacgtt
      301 cagtgtactt gtgatggggg atgatgttca gacacagcga aatcgtctgc gtgatgccct
      361 tcgtgctccc ggtgccgggg agctgcttca cccggattac ggcacgttaa atgtgctgat
      421 aaacagcttt gaaagtcgtt ataacgccgc tgaacagggc gtggttgagt tcaccattaa
      481 tgtcacgccg gtaagtgatg ataccgcacc tgctgtcaca caggacacgg ccgccattct
      541 ggagcagaag agtaccaccg cgctcgggca ggtttttgaa accctggaag cgggctggac
      601 ggtgatctct gacggaatgc acgatgtcca ggcaatgacc gaaaccatca gtgataaggt
      661 gtccgccctg gaaaatgccg tttccggcat ggggattgta caggatatca gtgcctttac
      721 tgccacgttc acggcgctga aagggaatgc cacggcatta ctgacagcac cttcccgcat
      781 ggcctcatct tttgcggggc ttttcagtgc cctgatcacc ctgccatcgc tgccgtcact
      841 gtcttcagga ggcctgaata cccggcccgg tggcagtatc ggccggacga catccgccgt
      901 ctctcaggga atgccgcaac tgtacaggac attatcttcc ctgcgttacg tgctggatga
      961 gcaggataat ccgcagactc tcatcggtct gacaccggca gcacaaaaga atattcgtct
     1021 gatacgggcg gtaatgcaga gtgctgccgt ggtggcccag gcacagaccg tgggaaaact
     1081 gctggatcag gttctcagtc aggaaaccct gccggacagt gacgcagccc accgtacctg
     1141 gcctgtctgg ctggaaagtt cagttgatct tcagcgcatt aaccgtgact taagcgaagc
     1201 gctcgaacgg caggtgatga cactgtccgg gcaggggtat accgccacgg cgctgacgct
     1261 tcgtgatgcc agactggcac tgacagagga tctgaataca cggggagttc agctacccgg
     1321 tgcaacagta gtgactgtac gtaccaccga gcctgcactg gtgaccctgt accgtgccac
     1381 cgggaacagt accggctggc aacgttttgt gcgccgtaac ggtattgttg atccgctgtt
     1441 cattcccgga ggccattcag tggaggtgat tagtgagcag cagggttgaa ctgtatctgg
     1501 gcggtgagat tttttccggc tggctgacgg tgagtgttcg tcgctctctt gaacatctgg
     1561 cgggctcctt tgaactgggg gtaatgatgc ccggtgtacg ccttccgtca tccgtccgtg
     1621 ccggtcagtc tctggaattg cgcattgacg gtcagcctgt gatcactggc tggctggatc
     1681 aggtccggca gcgcatcagc gccacgcgtt ttcagatcac gctcagcgga cgggataaaa
     1741 ccggtgacct ggtggactgt tcagccattc atccgggcag ccagtggagg aaccgcacgc
     1801 tggagcacat tgcttcagat ttgtgtgctc cgttcggggt cacggtgcgc tggcaggtaa
     1861 atgatgcaac ggcagcccgg cccttttcca ccttcacact ggaaaattca gaaaccgtgg
     1921 cagatgcgct gacacgggcc gcgagacaca gaggcgtcct ggtaaccagt aatgccgccg
     1981 gtgaactggt tttcactcag gccggcagtc agcgtggcga cacgctgacg ctgggcgaaa
     2041 atctgctgga tttggatcac aacgtggatc accgtctgcg ccacagtgaa taccgtgtac
     2101 gggggcacgg gcgtggtggt ggtcatgccg gggatgcact gacagccgga acgctggccg
     2161 caccagttgg tacggtgaca gacagtgcca tccaccgtta cagaccgaaa attgtgctgg
     2221 cggatcatgc tgttgatgca gacggtgcac gccagagggc tgtccgggaa atgcgccggg
     2281 cggttgcccg ctctgtgcgc ctgacagcca ccgtgcggca ctggtttcgg gagaacggcc
     2341 agttgtggga tatcaacctg ctgacggctg tcacggctcc ccgcaccgga gtggaagagc
     2401 gtgatcttct tgtctgtcag gtggagtttt cgctggatgc aaatcacggc gaaaccaccc
     2461 gtctgattct ggcaccccgt gacggcttta ttgttccggc agagccggga aacagcggaa
     2521 gcggaaatgc gggtgacgtg gacgccttcg tgcgggcaca gatgaaaaaa caggggatta
     2581 aattcaatga tgaatgacga agtcatcagc cgccttctgg cccccgtgat gcggggtgtt
     2641 cgtctgctgt tcgggcgtgg tgtactgacc ggcacaacgg acacgctgaa aatccagaat
     2701 gtgcaaatca ccggtatgga tggtgaaacc tttgatgacg ttgaacgccc ccagcagtac
     2761 gggcaaatca gcgtccccct gcctggtgcg gaagtttttc tggcctgtgc tggcggacaa
     2821 cgggatcagg ccgtggtgct tgtggtggaa gaccgccgca gtcgcccgac cggacttacc
     2881 gccggagata ccggcgtgta tcaccatgag gggcaccgta tacgtctgac aaagaacggc
     2941 cggatcattg tgacctgtaa gacgctggag atttacgccg atgaaggtgt tcaggtggat
     3001 acaccggagg ctcactttac cggtaatgtc acagtggata agaacctgca tgtcaaaggc
     3061 aatgtgtcca ttgacggcac cggaagatca caggggacgt tcacgatgtc cgaagcggtt
     3121 attgccggga tcacctattc aggtcatgtg catcacgata acggtgaagg cagcaaaacg
     3181 ggaggaccag agaatggctg atattgctgt cgtctgggat cagggttgtg gttccctgca
     3241 actgaacggc gcagatcttc tgacggataa cagcctgctg actgcggtca ttatttcact
     3301 gtttacggac aggcgggcgc tggattccga tgaaatccct gacggcaccc gtgaccgtcg
     3361 gggatggtgg ggagacagtt tccgggagcg ccccattggc tcccgtctct ggctgttaag
     3421 ccgtgaaaag acgctgtcct ccgtggtcag ccgtgcacag gcctatgctg atgaagcgct
     3481 ggcgtggctg cataaaagcg gtgctgccac atccgtggta tgtcatgcca tgcgtgtggg
     3541 gcatgatcgc ctttcgcttt ccgtgaaaat caccctgccg gacggaagca gacatccgat
     3601 gattttttat gctgatatga agggggaatg atgccttatc agcccttacc actggcgcaa
     3661 ctgatcacac agacacaaca ggatatcagc cagcgcctgc ccggttcgca gccgggcgtg
     3721 aatgaaacca ccctgaatgc cattgcgtat gcactggcgg gtctgtcagc acaggagcat
     3781 gaacatctgg cctggatctc ccggcagata attccgacag aagctgatga agccgaactc
     3841 ctgaaacact gcgcattctg gggtgtcatc cgtaaaccgg cttcccgcgc tgacggaccg
     3901 gtacaactga tgctgaccac ggatgcaggg atcacggaag gcgtactcct tcagcgaagc
     3961 gatggtgttg tgtaccgcat caccggctct gcgaccggaa aagccggaac actgaatgtt
     4021 aatgtggagg cggaaagtgc ggggcgcgcc ggaaataccc cgaccggaac ccgcctgtcc
     4081 tttatcacgc cacaggcggg catcaaccag acagccacgg ttaccggcac gggactcacc
     4141 ggtggtgcgg atgtggaaac ggtgccggag ttgctgtcca ggctggtatt ccgggtacag
     4201 aacccgccat cagggggaac acagtatgat tttgaacgct gggcacggga agtaccgggc
     4261 gtgacgcggg catggtgtaa gcctgaatgg cctgaggcgg gtagtgttgg tgtgactttt
     4321 gttcaggata ataaccctga cattttcccc ggagaaggtg atgtgaagcg ggtggcggat
     4381 tatatccgca gtcatgatga tccggcgacg ggccagcccg ttggtcagcc acttgggccg
     4441 acaatcagcg tgtttaagct gaccaataag ccggtggcgt ttgagatcag gattgtaccc
     4501 aaaacgccgg aaaatcaggc tgccgtaaaa caggcattaa cggacctgct ttataacgaa
     4561 tcgcggccgg gtggacttgt attgccttca tcattctggc gggctgttgc aggggtgaaa
     4621 ggactggagg attttgaagt tcgcagtccg ctgaagtccg tgatggccgg agatacagag
     4681 ttgctgaccg tgggggaaat cacatggctg taaccctgac cccgcatcag cgcgccctgt
     4741 tgcagttgct gcctgacggg ctggcatggg ataagcggcc gtcatccgtt cttgcagctt
     4801 tgtgcctggg cctcagtcat tccacggagc gtgtttcctg gaccggtaac cagatgctgg
     4861 cagaacgttt tcctgattca tcccgtctgc tgctggaaga ctgggagcgt tatctggggt
     4921 taccggaatg tgatatgacc ggcgcaacca ttcaggaacg tcagcgttat gcagggaata
     4981 aataccggat gaaaccctct cttaaccgtg aattttatat ccggtttgcg gcagagtttg
     5041 gttatgaaat agatatccag ccatcaccgg attcacagtg ggtcagtatt gtcacgataa
     5101 acagtgaaac cggctaccgg aatatgaatg tgctggatga tattctcacg ccgctgcgta
     5161 tttatgaggg cggtgcgctg gaatgtattc tgaaccgtta taagcctgca tggcagacgt
     5221 ttatttacgt atatgcaaac agtcacgaag aggagaatat ttaatgtt
//
LOCUS       DAZFVO020000243         5206 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFVO020000243 DAZFVO020000000
VERSION     DAZFVO020000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.245"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            58..2574
                     /locus_tag="ACHKNB_RS23840"
                     /old_locus_tag="pgaptmp_004768"
     CDS             58..2574
                     /locus_tag="ACHKNB_RS23840"
                     /old_locus_tag="pgaptmp_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198139.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLVLVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVAGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEALLPELPA"
     gene            2650..3111
                     /locus_tag="ACHKNB_RS23845"
                     /old_locus_tag="pgaptmp_004769"
     CDS             2650..3111
                     /locus_tag="ACHKNB_RS23845"
                     /old_locus_tag="pgaptmp_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HGU4198140.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKMLTDP"
     gene            3247..3336
                     /locus_tag="ACHKNB_RS23850"
                     /old_locus_tag="pgaptmp_004770"
     CDS             3247..3336
                     /locus_tag="ACHKNB_RS23850"
                     /old_locus_tag="pgaptmp_004770"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4198141.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            3514..4335
                     /locus_tag="ACHKNB_RS23855"
                     /old_locus_tag="pgaptmp_004771"
     CDS             3514..4335
                     /locus_tag="ACHKNB_RS23855"
                     /old_locus_tag="pgaptmp_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HGU4198142.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4335..4535
                     /locus_tag="ACHKNB_RS23860"
                     /old_locus_tag="pgaptmp_004772"
     CDS             4335..4535
                     /locus_tag="ACHKNB_RS23860"
                     /old_locus_tag="pgaptmp_004772"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198143.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            4669..5142
                     /locus_tag="ACHKNB_RS23865"
                     /old_locus_tag="pgaptmp_004773"
     CDS             4669..5142
                     /locus_tag="ACHKNB_RS23865"
                     /old_locus_tag="pgaptmp_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HGU4198144.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1247 a         1356 c         1392 g         1211 t
ORIGIN      
        1 cttcaacgcc acattcgctg gcattaacaa taacatgata ttcatcacgg agtgactatg
       61 ttacagatag tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg
      121 ttcagagcat taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc
      181 ggcccggcct tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc
      241 tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt
      301 gatggtaaac atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc
      361 accgctctcg ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac
      421 gggatcacac cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact
      481 gcctttttcc ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt
      541 tcaccgtcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtaacgctg
      601 ctatgggcgg ccagccagat ccaggcgctg aaaaaactga cctgtgccat cagccagaca
      661 ctggaagaac aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac
      721 agccttcctg actcactgac ggagcgcatc tggctgacgc tcatttctca acggatttcc
      781 cggggagagc tgagggaatt tgaactggca gacggaaact ggttactgaa caatgcctgg
      841 tatgaaagaa acatggcagg gtttaacgaa cagttgaaag agaacctgtc attcacacct
      901 gatgaactga aaacgctctt ccggaaccgc ctgaatttat caccggaagc gaatgacgat
      961 tttctcgatc gttgcctgaa cggcggtgac tggtatccct tttcagaagg tcgccgtttt
     1021 gtatcattcc atcacgtgga tgagcttcgt atctgtgcct cctgcgggct gacagaagta
     1081 catcatgccc cggaaaatca taagccggat ccggaatggt actgctcctc tctttgtcgc
     1141 gaaacagaaa cactgtgtca ggaaatttat gaacgccctt acaacagctt tatttccgat
     1201 gcaacggcga atggtctgat tctcatgaaa ctgccggaaa cctggagtac aaatgagaaa
     1261 atgtttgctt ccggagggca gggacatggg tttgccgctg aacggggaaa ccatattgtc
     1321 gacagagtcc gtctgaaaaa cgcacggatc ctcggtgata ataatgccag aaatggagca
     1381 gacagactgg tcagcggaac agaaatccag acgaaatatt gttcaactgc agcccgtagc
     1441 gtcggtgcgg cattcgacgg acaaaacgga cagtatcgtt acatgggaaa taatggcccc
     1501 atgcaactgg aagtcccccg tgatcagtat gccggcgctg ttgaaaccat gaggaataag
     1561 atccgcgaag gtaaagtacc cggtgtaacc gatcccgcag aagcgtcccg gctgattcgt
     1621 cggggacatc tgacttatac tcaggcccgt aatatcaccc ggttcgggac catcgaatcg
     1681 gtcacttatg atattgccga gggttcggtt gtcagtctgg cggccggagg gatcagtttt
     1741 gccctgacgg catcggtctt ctggctcagc accggcgatc gcgatgctgc cctgcagaca
     1801 gctgctgtcc aggcaggaaa aaccttcacc cgcacactgg ctgtctacgt cacaacccag
     1861 caactccacc ggctcagtgt tgttcagggt atgctgaagc atattgattt ttcgacggcc
     1921 agcccgactg tccggcaggc gcttcagaag gggaccggtg caggaaatat cagtgccctg
     1981 aacaaagtga tgaagggtac gctggtgaca tctctggtac tggtcgctgt cacaaccggc
     2041 cctgacatga tcaaaatgtt gcggggacgg atctccggtg cgcagttcat caggaatctt
     2101 gccgtggcat cttccggtgt ggcaggtggt gctgtcgggt cagtggcggg cgggatactg
     2161 ttcagtccac tgggaccatt tggtgcactg acagggcgtg tggctggcgg tgttctgggg
     2221 ggaatgattg cctccgctgt atcaggaaaa attgccggag cgctggttga agaagatcgc
     2281 gtcaaaattc tggcaatgat tcaggagcag gtgacatggc ttgccggcag tttcctgctg
     2341 accggacatg agattgaaaa tctgaacgcg aatctggccc gtgttatcga tcagaatgct
     2401 ctggagatca ttttcgccgc cggtatacaa caacgggccg cgaccaatat gttaatcaaa
     2461 ccactggtgg tcagtatcat caggcaacgc cccgtcatgg aatatgatgc atcccatctc
     2521 ggcaagatgg ttaaccgact ggaagaagca ttactcccgg agttaccggc ataacggcta
     2581 ttctgcgcct tttacgtttt atcacctgat ttgtcagtgt catcgtcatg actcagagag
     2641 gtaaatacca tgattcacct gttcaaaacc tgcatgatta ccgccttcat tctggggtta
     2701 acgtggtctg ccccactccg ggcacaggat caacgttaca tcagtatacg caatacagat
     2761 acgatatggc tcccgggaaa tatttgtgct taccagttcc ggctggataa tggcggaaac
     2821 gatgaaggat ttggccccct caccatcact ctgcaactca aagacaaata tggtcagacg
     2881 ctggtgacca gaaaaatgga aacggaagcc tttggtgaca gtaatgccac gcgaaccaca
     2941 gacgcatttc tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat cattaaagca
     3001 actgaagaaa gtaacggcca tcgtgtcagt ctgccgttat cggttttcga tccccaggac
     3061 taccatccac tgctgattac cgtttccgga aaaatgttaa ctgatccctg aagtcagttt
     3121 catgcttcag cctctgccac aacgtatggc agaggctatt tatcaggaga aaccagatat
     3181 gccgggttac acacagagta tgtcctggct gaaggatctt tctcatacgg gcaggcggtt
     3241 gctgtcataa ccgcttaccg caatgtgttt atccaggatg acccgggaat gcatttccgt
     3301 cgggttatcc gcaacgcgga aggtcagcga cgctgacgct gccggaactc tgaagctgat
     3361 gccggaaaac agcttaatgc gtggctcgcc agtggagggc ttctcagaca ataaacgtca
     3421 ttgatttcgt ccatcaggcc gcgtcttctc cgggagacgc ggctttttca tttattgcac
     3481 cagtaaatct taaccaccga taaggagcaa agtatgcgat tagtcagtcg ttttggttat
     3541 gctgcaaacc agatacgccg tgaccgtccg ctgacacatg aagagctgat gcaccatgtg
     3601 ccggggattt tcggggaaga gaaacacaca tcccgaagcc agaactatac gtacatcccc
     3661 accatcaccg tactggaaag tctgcagcgg gaaggcttcc agcccttttt cgcctgccag
     3721 acccgtgtgc gcgacccggg ccgccgggga tacacaaaac acatgctgcg tctgcggcgg
     3781 gccggagaga taaacggaga acatgtccct gaaattattc tgctcaactc ccatgacggt
     3841 acctccagct accagatgct gccgggttac ttccggttcg tctgccagaa cgggtgcgtc
     3901 tgtggtcagt ctctggggga agtgcgtgtt ccacaccggg gaaatgtggt gcagaaagtc
     3961 attgaagggg cttacgaggt ggtgggcgtt tttgaccgga ttgaagagaa acgtgatgcc
     4021 atgcagtcgc tggtcctgcc gccaccggca cgccaggcgc tggcacaggc ggcactgact
     4081 taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac gccacgacgt
     4141 cgggaggatt acggtaaaga cctgtggagc gcatatcaga ccatccagga gaatatgctg
     4201 aaaggcggga tttccggtcg cagtgcaaaa ggaaaacgta tccacacccg tgccattcac
     4261 agcatcgaca ccgatattaa gctcaaccgc gcattgtggg tgatggcaga aacgctgctg
     4321 gagagcatgc gctgatgaaa catatccctg aaggcgtgat tcaggcaccc gtatcacccg
     4381 acgccccgga ggcgctgccg ggatgtcgtt ccatttctga tgatgacctg tgcgcctggt
     4441 gtacccgtct gctttaccgt cccggagaac tcagtctgtg cagactgagt acaggcaatg
     4501 gcatctggcc atccgtctgt gaccggaacg gttaacggct gtccggaatt tcagccgaat
     4561 atcattcatc cctgatattg ccgggtctca cggtcagtga tgaacgtgat gttgtcagcc
     4621 cccggccttt atttcctctc atttattcac attacaagga tttcacacat gaaaacattg
     4681 tctcagaaca ccacctcttc agcctgtgca ccggagactg gcctgcaaca actggttgcc
     4741 accatagtcc ctgatgaaca gcgcatcagc ttctggccgc agcattttgg cctcattcca
     4801 cagtgggtga ccctggagcc ccgtgttttc ggctggatgg accgtctttg cgaagactac
     4861 tgtggtggta tctggaatct gtataccctg aacaacggcg gggcatttat ggcacccgaa
     4921 ccggatgacg atgatgacga aacatgggta ctgttcaatg ccatgaacgg taaccgcgct
     4981 gaaatgagcc cggaagccgc cggcattgcc gcctgtctga tgacgtacag tcatcacgcc
     5041 tgtcgtacgg agtgttatgc catgacggtc cattattacc ggctgcggga ttacgccctg
     5101 cagcatccgg aatgcagcgc cattatgcgc atcatcgact gagcaggaga agtacaatgc
     5161 aacaactttc ctttctgccc ggcgcgatga cgcccggcga gcgcag
//
LOCUS       DAZFVO020000244         5118 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFVO020000244 DAZFVO020000000
VERSION     DAZFVO020000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.246"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..3045)
                     /locus_tag="ACHKNB_RS23870"
                     /old_locus_tag="pgaptmp_004774"
     CDS             complement(<1..3045)
                     /locus_tag="ACHKNB_RS23870"
                     /old_locus_tag="pgaptmp_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4198145.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSSAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     IQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMKAST
                     DNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQ"
     gene            complement(3379..4251)
                     /locus_tag="ACHKNB_RS23875"
                     /old_locus_tag="pgaptmp_004775"
     CDS             complement(3379..4251)
                     /locus_tag="ACHKNB_RS23875"
                     /old_locus_tag="pgaptmp_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HGU4198146.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         1080 a         1391 c         1371 g         1276 t
ORIGIN      
        1 ctgaacgccc agggtgatat tttcccggac acgggcttcc agtccggcct gcaggtccag
       61 tgacgtgcca ttccgggacg gtgagaacgt catgttactg ccggctgcag ctgtacccat
      121 gctcatgtcc ccccgggagc tgaaggtgcg gataacagaa ggttgtaccc accagttcac
      181 aggcagttca ctcacaccgt gttttgtact gtcgcgcagg gtgtcacggg atgaggtgcc
      241 ttcaccaaag gtcatatcgt tgtggctgcc cagacggaaa ccggcacgca catgttgtgc
      301 actgccatac ccgaacttca cataaccggc gttatcctgg ccgtcatcca gggagagtcc
      361 ctgccaggtg tactgcagtt gcggctccag catcagattg tcagtgatac tgaagggcag
      421 accggtttcc agtgaaccca gccagcccca cccccgggcg cggaagtcgt tattgtccgt
      481 tgacgctttc atgctgtggc gggttcccag ggccacaatg tcagcccaca ggccggagga
      541 tgtatgtgtc agattcaggt atccgccaag gctgccggca tcatcccgga ccgtgccggc
      601 acgggaggcg tcatcatcct taacatcaac ggaagaatgg cctgcagcac catatatccc
      661 tgcggtcaca gacataccgg caacctctgt tctcagcagg tcaccctcca ggcggacgaa
      721 gccatagctg ccgctgcttt ccggcgtggc cccacgggca ataccgccgt tgttatcgtg
      781 accgagatgg ccgccctgaa tgctgagacg gacgctgtta ttttcaccgt ttacaccggt
      841 ctgatggctg cgggagcctg ccagaatccg gtcatagtcc attgcctgtg tcagcatgga
      901 ggcgtacagg gggacttcag cacgataacg ttcttcactg cgcagatacc agctctcatc
      961 actgtcccgg ttgagggtgt agttaaaggc cccggcctgc agcatattcc cctggacaaa
     1021 agccccttcc tccgtggtgg caccgttaat ggcctcaacc acctgaatac ccttaccggt
     1081 ggtcgccagc cccgtaccac tgttgccggc gttcaccaga ttcaggatgg tttttccggt
     1141 tgccctgcca ccgtcaatga ccagtctgtc agcattgttc tgcgccatat ccgggcgtac
     1201 acgcaggctg atggtgccat tctgcccgtt caggtttttc acctgcaggg ttgtcggaac
     1261 gaacttccct gtgcgggcgg aggtgaaatg aatctgtccg gcatggctga ggtcatccac
     1321 caccgactga accgtggcgt tatcggggat attccaggtg gcaccggatg tcagggtgac
     1381 attcgtgggg tcaatggcac cgttcagcac tgtgctgccg gtcagcttca gggccgtgcc
     1441 gcgctgagcg atgacatccg tggtgacggt actgtcgttc agcgtcagcg tgccttcatt
     1501 caggttgacg gttttctgga tgagtgtggt gttgctgaca gtgagtgtgc cacttcctga
     1561 tttttccacc ctgccgttac cggtaagagc gcctccctgc aggagtgcat caccttcatg
     1621 gatggtcagg gtatcgttat tcaccgtttt cccggaaagg gtaagcgtgg caccgttatt
     1681 cagcgtggtg gtgcccgcca gcgagccccc tctggcggtg aacagtccgc catttaccgt
     1741 ggtatccgtg gccgtatcac cggcgttcag tgtgaaggaa ctgccttttt ccagcatcag
     1801 ggcatccgcc tgaccgcctc cgatacggaa tgccgttccg tcgctccggg taccactgac
     1861 agcggcaccg gaatcggcca gcagtacacc gccattcccc atggatacgg tggtggcggc
     1921 accaccgttg cggacatcca gcgttccgcc atcatccaca cgggtattag tggctgtgtg
     1981 tccgctcagc acatccagac gtccgccatt ttccagtacg acattatcag ccttacccgc
     2041 cacaacggag aatgctccca ggcggtttgt accggtgacg gttgcagctg aactggtaac
     2101 cagtgcaccg cccgtgttct gggtgacatc agacgcttta ccgccggcat tcacctgcag
     2161 ctttcctttc tggttgatgg tggtatgcgc ggcagttcct ccttccttaa tcacctgcca
     2221 gccatcacgg tttatcagcg tctctgttgc cgtgccaccg ttgtgtacat actggttacc
     2281 cccctccagc cgggtgttat gtgcctctcc gtgtaccgtc tggtcaccac cggcgtaaat
     2341 gagggtgtcc cgtgccatcc ccgaagacca gataacctgc cggccatttt tgttgatggt
     2401 ggtggattca gccgtccctc cgaccatctg accgctggac acattttcag actccggacc
     2461 accttctgca ccggtgttga cgatggttcc ggttgccagt ccgccatgtt ttatggtctg
     2521 gtaaccatcc tggttaataa ttgtaccggc agctttccct cccccgtgta cccactgctc
     2581 acctctgtta tccagcgtgg tgttcaccgc cagtccgtta aggctctgcc ctccgccatc
     2641 acgaataacc gtatcactgg cagttcctcc tgcctgcaca atctgacgac catttgcggt
     2701 gacagtggtg tttctgcctg tgccacccgc gtttatccat tgcccgccgg tgttttcgtc
     2761 actgtccggc cccagctcaa gcccggtact gacagtcacg ccatcagctg ttccggatac
     2821 aaactggttg tcatggttta ccagtgttcc gccattcact gtttcacccg ggtgcacaac
     2881 gatgtcagca gccagcaccg ggagtgacgt gaccgcggca agagacagtg caaccgccac
     2941 accgccacgt ttaccccgtg cgcgggccag ttcggaggca accacgaaag cgcccgtaat
     3001 gtgattccat accagcctgt agcaggtatt cagatgtcgt ttcatcagca tttccttaat
     3061 cagagtgagg gtgaataaaa aagccggtat ccacagatgt ggataccggc aggtaaaggg
     3121 ttacagtgcc agaggtccgg ctgacggaag tttctgaatt gtggttctgt tgttccctga
     3181 atctcatgtc tgtgtgtatc tgcgcacgtt gctaacagga gttattatta gcataacgat
     3241 cgatattaac gatcggtgaa ataggtatcg atcgttttat tctattgtca agctattgca
     3301 cggacaagag agacagtaac gaaaacagcg cttttgtgag cagacccaga ggcagggagg
     3361 acatcatcca cgacaggttc agaagaaaat ccagttccat accgcacggg caacagagac
     3421 caccgtgtca cgaacagcgc gcagggccgt acggacaaca gaggcaacac agacgctctc
     3481 cgctgtgtca aatatccggt ccaccgcacc ggtaaactgt tcacgggcct gagagcggac
     3541 agattccgtg cgcagcccgt cctgcagtcg ggtcatcagg ggactggcgg catggtcggg
     3601 aagcgctgtc atgagcgcac tgaccagcgt atccagttcc cagccggtgc gggccgatac
     3661 ggccacaacc ggatgtacgg gccggaacag acggaatacc gcctccgttt tctcccgaat
     3721 gttctgtgcc tgtgcgggag aaggctgaat accggccata tcccattcat ggcagggctc
     3781 cgttttatcg gcctgcgtca ccacaaacag tacccgctga tgtccccggt gcagtatgtg
     3841 tcgccagaaa tactcatcca cagacagggc acggtcatcg gctttaatca gccacagtac
     3901 caggtccagt tctggcagaa tgtcacggta cagggcctca tactctgcat ccctgtcccg
     3961 gctctcgccc accccgggca ggtcagtgat aaccatgctg tgaccgtgac cactcagacg
     4021 gaagcgctgc acttcccggg tgccggcgtg aacatcactg accggtgtga cctccccctg
     4081 aaacagtgca ttacagagtg aagatttacc ggccccgctt ttacccataa tgccaatcac
     4141 gggttcgtga cgggtgagtt tgcgcagatg ttccaggatg tgacgtgaaa gcgagtaagg
     4201 cagggaagaa aggggttttt caattgcctc aatggcatca gacggattca tacatgtatt
     4261 cccggtgaaa aaagacaaaa ccccgcgcac cggaaaggtg gcgggggatc cacggagata
     4321 atatctgtct gaaattgttt ggaatcgatg gggatgttgc gtattaacat gaaggagggc
     4381 aggagttatc cgttactggg tgatatcctt ccatgaaaat acgcggttat tgttaaacag
     4441 caggctgatt actccgtaaa ggagaaaaat attcatcttg cagcctgatt atcaggtgtg
     4501 tttatgttta ctgaggtgag tatttttcag tatattgtcg gggcagtcag tgggagatat
     4561 taaatttacg tttagagtta tcttgcttct gctgtgtatt tttataagag tgcgcacgta
     4621 atgatatctg ttgataccat gttgttaagt gtttcccttc ctggtcatgc gtaatgatta
     4681 aatgcttttg tgacaccatt cttccggcat gaatatttat ccagttccct tttttatttg
     4741 tgtagtgata ctgtcggaat agcgttcagt aatgtgaagt gtcctgttaa aaatctttaa
     4801 taaatgtgtc atcctggtca ggaaatatct gctgagatat tattagttgg tggtgttttt
     4861 actgagtaat agctggcttt taattcctga actgaaatac tgtcgctcgt cagggtttaa
     4921 ttaaaactgg atgatgatgt gaaaatgaag gctggttttt gtgtttgtcg aagaagtgtt
     4981 gtttttatcc attctcatca ttggctggcg attatacaga agtatttgaa gaaaaaatgg
     5041 tgcattttat ctgtgccggc agggaggggg attttcctga tatgtcagta ttaaatggtt
     5101 tttgttattt ataaaaat
//
LOCUS       DAZFVO020000245         5045 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFVO020000245 DAZFVO020000000
VERSION     DAZFVO020000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.247"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            121..1035
                     /locus_tag="ACHKNB_RS23880"
                     /old_locus_tag="pgaptmp_004776"
     CDS             121..1035
                     /locus_tag="ACHKNB_RS23880"
                     /old_locus_tag="pgaptmp_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HGU4198147.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            1107..2354
                     /locus_tag="ACHKNB_RS23885"
                     /old_locus_tag="pgaptmp_004777"
     CDS             1107..2354
                     /locus_tag="ACHKNB_RS23885"
                     /old_locus_tag="pgaptmp_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HGU4198148.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2796..2870)
                     /locus_tag="ACHKNB_RS23890"
                     /old_locus_tag="pgaptmp_004778"
     tRNA            complement(2796..2870)
                     /locus_tag="ACHKNB_RS23890"
                     /old_locus_tag="pgaptmp_004778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2835..2837),aa:Arg,seq:cct)
     gene            complement(2946..3878)
                     /locus_tag="ACHKNB_RS23895"
                     /old_locus_tag="pgaptmp_004779"
     CDS             complement(2946..3878)
                     /locus_tag="ACHKNB_RS23895"
                     /old_locus_tag="pgaptmp_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGU4198149.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4172..4927
                     /gene="mlaA"
                     /locus_tag="ACHKNB_RS23900"
                     /old_locus_tag="pgaptmp_004780"
     CDS             4172..4927
                     /gene="mlaA"
                     /locus_tag="ACHKNB_RS23900"
                     /old_locus_tag="pgaptmp_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HGU4198150.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1208 a         1115 c         1267 g         1455 t
ORIGIN      
        1 aatgttaacg ttaactatga cgttttgtga tcgaatatgc atgttttagt aaatccatga
       61 cgattttgcg aaaaagaggt ttatcactat gcgtaactca gatgaattta agggaaaaaa
      121 atgtcagcca aagtatgggt tttaggggat gcggtcgtag atctcttgcc agaatcagac
      181 gggcgcctac tgccttgtcc tggcggcgcg ccagctaacg ttgcggtggg aatcgccaga
      241 ttaggcggaa caagtgggtt tataggtcgg gtgggggatg atccttttgg tgcgttaatg
      301 caaagaacgc tgctaactga gggagtcgat atcacgtatc tgaagcaaga tgaatggcac
      361 cggacatcca cggtgcttgt cgatctgaac gatcaagggg aacgttcatt tacgtttatg
      421 gtccgcccca gtgccgatct ttttttagag acgacagact tgccctgctg gcgacatggc
      481 gaatggttac atctctgttc aattgcgttg tctgccgagc cttcgcgtac cagcgcattt
      541 actgcgatga cggcgatccg gcatgccgga ggttttgtca gcttcgatcc taatattcgt
      601 gaagatctat ggcaagacga gcatttgctc cgcttgtgtt tgcggcaggc gctacaactg
      661 gcggatgtcg tcaagctctc ggaagaagaa tggcgactta tcagtggaaa aacacagaac
      721 gatcgggata tatgcgccct ggcaaaagag tatgagatcg ccatgctgtt ggtgactaaa
      781 ggtgcagaag gtgtggtggt ctgttatcga ggacaagttc accattttgc tggaatgtct
      841 gtgaattgtg tcgatagcac gggggcggga gatgcgttcg ttgccgggtt actcacaggt
      901 ctgtcctcta cgggattatc tacagatgag agagaaatgc gacgaattat cgatctcgct
      961 caacgttgcg gagcgcttgc agtaacggcg aaaggggcaa tgacagcgct gccatgtcga
     1021 caagaactgg aatagtgaga agtaaacggc gaagtcgctc ttatctctaa ataggacgtg
     1081 aattttttaa cgacaggcag gtaattatgg cactgaatat tccattcaga aatgcgtact
     1141 atcgttttgc atccagttac tcatttctct tttttatttc ctggtcgctg tggtggtcgt
     1201 tatacgctat ttggctgaaa ggacatctag ggttgacagg gacggaatta ggtacacttt
     1261 attcggtcaa ccagtttacc agcattctat ttatgatgtt ctacggcatc gttcaggata
     1321 aactcggtct gaagaaaccg ctcatctggt gtatgagttt catcctggtc ttgaccggac
     1381 cgtttatgat ttacgtttat gaaccgttac tgcaaagcaa tttttctgta ggtctaattc
     1441 tgggggcgct attttttggc ttggggtatc tggcgggatg cggtttgctt gatagcttca
     1501 ccgaaaaaat ggcgcgaaat tttcatttcg aatatggaac agcgcgcgcc tggggatctt
     1561 ttggctatgc tattggcgcg ttctttgccg gcatattttt tagtatcagt ccccatatca
     1621 acttctggtt ggtctcgcta tttggcgctg tatttatgat gatcaacatg cgttttaaag
     1681 ataaggatca ccagtgcgta gcggcagatg cgggaggggt aaaaaaagag gattttatcg
     1741 cagttttcaa ggatcgaaac ttctgggttt tcgtcatatt tattgtgggg acgtggtctt
     1801 tctataacat ttttgatcaa caactttttc ctgtctttta ttcaggttta ttcgaatcac
     1861 acgatgtagg aacgcgcctg tatggttatc tcaactcatt ccaggtggta ctcgaagcgc
     1921 tgtgcatggc gattattcct ttctttgtga atcgggtagg gccaaaaaat gcattactta
     1981 tcggagttgt gattatggcg ttgcgtatcc tttcctgcgc gctgttcgtt aacccctgga
     2041 ttatttcatt agtgaagttg ttacatgcca ttgaggttcc actttgtgtc atatccgtct
     2101 tcaaatacag cgtggcaaac tttgataagc gcctgtcgtc gacgatcttt ctgattggtt
     2161 ttcaaattgc cagttcgctt gggattgtgc tgctttcaac gccgactggg atactctttg
     2221 accacgcagg ctaccaaaca gttttcttcg caatttcggg tattgtctgc ctgatgttgc
     2281 tatttggcat tttcttcttg agtaaaaaac gcgagcaaat agttatggaa acgcctgtac
     2341 cttcagcaat atagacgtaa actttttccg gttgttgtcg atagctctat atccctcaac
     2401 cggaaaataa taatagtaaa atgcttagcc ctgctaataa tcgcctaatc caaacgcctc
     2461 attcatgttc tggtacagtc gctcaaatgt acttcagatg cgcggttcgc tgatttccag
     2521 gacattgtcg tcattcagtg acctgtcccg tgtatcacgg tcctgcgaat tcatcaagga
     2581 atgcattgcg gagtgaagta tcgagtcacg ccatatttcg ctatcaggat tctgtgtgat
     2641 ggttacatcg cccggctcag ggctgtttag tcatcagcgc tttctgacag tgctgagatt
     2701 tcaacctgtt gcagtaaaaa tgagtagata taaggcaagt gtgctgccaa acccatcttt
     2761 tacggggtga aggtagattt cgtttgaagg gtatctggtg tcccctgcag gaatcgaacc
     2821 tgcaattagc ccttaggagg ggctcgttat atccatttaa ctaagaggac aatgcggcat
     2881 gagtataccc gctaatggac tgcggggtaa gtacgctgcc gctcgattgc ttaaaccctc
     2941 gccatttatg ctgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttgg
     3001 cgtgcttctg ctttacgctt gttgctcatg tcgttacgaa tctgtgcatg actcattaac
     3061 gcgaagataa aggtgccgcc gcagatgttc cccgctaaag taggtagtgc gaagggccag
     3121 atgaaatcgc tccagtgcag cgtgccgtta aacaccagat agaggatttc aacagaaccg
     3181 acaacgatgt gggtggtgtc acccagtgca ataagccagg tcatcaaaat aatcaccaca
     3241 atctttgccg cacccgctgc aggaaacatc caaaccatag tggcgatcag ccagccggaa
     3301 atgatcgcgt tggcaaacat ctcgctgggg gtgttcttca tcacatccat gccgattttg
     3361 acaaatgcat cgcgagtttc ttcattgaag ataggcatat attcaaatgc ccatgcagca
     3421 atacctgtcc cgagaatatt acccagcagc acgacgcccc ataatcgcat aagtaagccg
     3481 acgttgctca ttgtcggttt ttgcatgacg ggtagtaccg cagtcacggt gttttcggta
     3541 aataattgct ggcgggccat aatgacgata ataaaaccaa aggtataacc gagattctcc
     3601 agtaagaagc tgcctggcac tccttccagt tcgacatgaa atatcccttt tgccagtagc
     3661 gaagcgccca tcgacagacc cgccgcaatg gctgaccaca gtagcgccat tgcgtcgcgt
     3721 tccagctctt tttcaccatc ctggcggata tgctcatgaa ttgccatcgc ccgggagggg
     3781 agtcggtctt catctatttc tatttttttg ccgcgctctt tttcttcgct ctcaacttca
     3841 atttcgtcgc tgtgttgatc aattttgtcg ttgtccatgg tctcttctct tgaattagca
     3901 cgtatagcta aagcgtagcg gcttttttgc tcgcaactgg cgggagttac tctgaaaatg
     3961 tagaaaaggc tgcgtttgcc tttttctgtt tctatagaat caagtagcct acagggcggc
     4021 gattaccagg ctatgatcaa atcagcaaat cagggcgtct ggacatcagt tgacgtgctg
     4081 ttacaatcgc ccacacctaa acaggcggat acggtatcgt tccgtcatgg atggcaaact
     4141 gcataagcca taaaaaaaca gggagacatt tatgaagctt cgcctgtcgg cgcttgctct
     4201 gggaactacg cttctggtgg ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga
     4261 cccgttagaa gggttcaacc gcaccatgta caacttcaac ttcaatgtat tagacccgta
     4321 tattgttcga ccggtcgctg tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaacgg
     4381 tttgagcaac tttactggca accttgaaga acctgcggtg atggttaact acttcttgca
     4441 gggcgaccct tatcagggga tggtccactt tacccgcttt ttcctgaaca ccattttggg
     4501 gatgggcggt tttattgatg ttgcagggat ggcgaacccg aaactgcaac ggactgaacc
     4561 tcaccgcttc ggtagtacgc ttggtcatta tggcgtgggt tatgggcctt acgtccagtt
     4621 accgttctac ggtagcttca cgctgcgtga tgacggtggt gatatggcgg atggtcttta
     4681 cccggttctt tcctggctga cctggccgat gtctgtgggt aaatggacgc ttgaagggat
     4741 agaaactcgt gcgcagttgt tggattccga tggtctgctg cgtcagtcgt ccgatcctta
     4801 tattatggtg cgcgaagcgt acttccagcg tcatgatttc atcgctaatg gcggcgaact
     4861 caaaccgcag gaaaatccga acgcgcaagc gattcaggat gatttaaaag atatcgattc
     4921 tgaataagaa acaaataaaa aaggtgagtc gcaatactca ccttttttgt tgttatccct
     4981 caattaccac aagtaaaagg agataaaccg acaatgaccc ggacgtaatg atgtaaaaat
     5041 actga
//
LOCUS       DAZFVO020000246         5018 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFVO020000246 DAZFVO020000000
VERSION     DAZFVO020000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.248"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(39..350)
                     /locus_tag="ACHKNB_RS23905"
                     /old_locus_tag="pgaptmp_004781"
     CDS             complement(39..350)
                     /locus_tag="ACHKNB_RS23905"
                     /old_locus_tag="pgaptmp_004781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGU4198151.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            549..1247
                     /locus_tag="ACHKNB_RS23910"
                     /old_locus_tag="pgaptmp_004782"
     CDS             549..1247
                     /locus_tag="ACHKNB_RS23910"
                     /old_locus_tag="pgaptmp_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGU4198152.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1292..2191)
                     /gene="eamA"
                     /locus_tag="ACHKNB_RS23915"
                     /old_locus_tag="pgaptmp_004783"
     CDS             complement(1292..2191)
                     /gene="eamA"
                     /locus_tag="ACHKNB_RS23915"
                     /old_locus_tag="pgaptmp_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HGU4198153.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVVRPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2386..3573
                     /gene="ydeE"
                     /locus_tag="ACHKNB_RS23920"
                     /old_locus_tag="pgaptmp_004784"
     CDS             2386..3573
                     /gene="ydeE"
                     /locus_tag="ACHKNB_RS23920"
                     /old_locus_tag="pgaptmp_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HGU4198154.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3700..3795
                     /gene="mgtS"
                     /locus_tag="ACHKNB_RS23925"
                     /old_locus_tag="pgaptmp_004785"
     CDS             3700..3795
                     /gene="mgtS"
                     /locus_tag="ACHKNB_RS23925"
                     /old_locus_tag="pgaptmp_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HGU4198155.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3795..3899
                     /gene="mgtT"
                     /locus_tag="ACHKNB_RS23930"
                     /old_locus_tag="pgaptmp_004786"
     CDS             3795..3899
                     /gene="mgtT"
                     /locus_tag="ACHKNB_RS23930"
                     /old_locus_tag="pgaptmp_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HGU4198156.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4014..4904)
                     /gene="dgcZ"
                     /locus_tag="ACHKNB_RS23935"
                     /old_locus_tag="pgaptmp_004787"
     CDS             complement(4014..4904)
                     /gene="dgcZ"
                     /locus_tag="ACHKNB_RS23935"
                     /old_locus_tag="pgaptmp_004787"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HGU4198157.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPMDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         1373 a         1194 c         1053 g         1398 t
ORIGIN      
        1 aaagttagtt caggtaatag tttaataata ttagattctt actcttgcag tagtttcatc
       61 gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt ctttcatctc gataacggct
      121 acaggcattt tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcggg
      181 ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc
      241 aatgcggaaa tattaacttc aagtgaaata gcgttagcat gatcaatcat acgtttaacc
      301 agcatactgg ttgacatgcc cgcagcgcaa actaacaata tttttttcat attttccctt
      361 actggtatat aacagattac attgtttaaa tatcatatcc cgcagtagct atacgtcctg
      421 gtacattgat cacagtaata cagtgataaa gttgttcaac atcagggtga ttattaaacg
      481 actcggccat acgataaacc aatcaggact caactccttg ttttcaatgg ctcttaaaag
      541 gtgcaaaagt gatttttcaa aagattgccc gcttacttaa atccgaaatc aatggcaatt
      601 catggcatgt tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt
      661 cgcgtaatac cctgcgtaag gcattatcct tgctggaagg cgaaggaatt attcacagaa
      721 agcatggttc aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga
      781 atagtttcag tgaaattgcg cataaaagcg gcaaagaggc agggagtcag attatgaaat
      841 ttgaagtgca ggatgcttca cccactattg cgactgagct gaatttagtg actggtgagc
      901 aggtttatta cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa
      961 cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc acatatgcaa aaatccaaat
     1021 tttcatatat tgagaatgaa tgcgggatca aaatcattgg cacctttgaa actttctccc
     1081 ctacttttcc taccccagaa atcgccagta ttttacggat cagcccacga gatcccatac
     1141 ttaaaattca gacccaggct gtggacagta actctattcc gctggattat tcgttacttt
     1201 acagcaatat tttcgagttc caggtaaagt actttttccc gcgataaaag cagacgcaca
     1261 gagtgctgac atcacattct gtgcgtatgg tttagcctct aacctttacc gctttacgcc
     1321 accgcaagcc aaatacattg atatacagcc cggtcataat gagcaccgca ccgaaaaatt
     1381 gtagacccgt taagcgttca tccaacaata gtgccgcact tgccagtccg actacgggca
     1441 ccagtaacga taacggtgca acccgccagg tttcatagcg tccaagtagt gttccccaga
     1501 tcccataacc aacaattgtc gccacaaacg ccagatacat cagagacaaa atggtggtca
     1561 tatcgatagt aaccagactg tgaatcatgg ttgcggaacc atcgagaatc agcgaggcaa
     1621 caaagaaggg aatgattggg attaatgcgc tccagattac cagcgacatc accgccggac
     1681 gcgttgagtg cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg
     1741 ccgccagggt caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga
     1801 ttaacaccag tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgcaggc
     1861 gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc tgtaacacca
     1921 gcgaagccag tccagcaggc ataccaaaat taatggcgca gaacagaaag gcaaactgcg
     1981 caaaactgat ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtacga
     2041 caaaaaagat agccggaaaa gcgaccagca taaagcgcaa accggccagc atcagcggtg
     2101 gcatgttatg aagccccact ttgatgacca caaaatttag cccccatacg accactacca
     2161 gtagcgccaa caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta
     2221 agcaatcaac ttagctgaat ttacttttct ttaacagttg attcgttagt cgccggttac
     2281 gacggcatta atgcgcaaat aagtcgctat acttcggatt tttgccatgc tatttcttta
     2341 catctctaaa acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta
     2401 cgacgctcta ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct
     2461 acgctgccat ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc
     2521 ggttatgcga tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc
     2581 ctggcggata agttcgacaa gaaacgctat atgttactgg caattaccgc cttcgccagc
     2641 ggttttattg ccattccttt agtgaataac gtgacgctgg tggtgctctt ttttgccctt
     2701 attaactgcg cctattccgt ttttgctacc gtgttgaaag cctggtttgc cgacaatctt
     2761 tcgtccacca gcaaaacgaa aatcttctca atcaactaca ccatgctaaa cattggctgg
     2821 accatcggtc cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct gcccttttgg
     2881 ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt aaagcgcagc
     2941 gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat
     3001 aaagcactgt tgtggtttac ctgctctggt tttctggctt cctttgttag cggcgcattt
     3061 gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc cgaaaaggtg
     3121 gtcgcggttg ttcttccggt taatgctgcc atggtagtta cgttgcaata ttccgtgggc
     3181 cgccgcctta acccggttaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc
     3241 atcggtctgg ttggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct
     3301 gcggtattta ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat
     3361 attgcgccgc caggaatgaa agccagctat ttttctgccc agtctttagg ctggcttggt
     3421 gctgcaatta acccattagt gagtggcgta gtgctaacca gcctgccgcc ttcctcgctg
     3481 tttatcatct tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa agggattcgt
     3541 gcaaaaccgt gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aaaaatttaa
     3601 ttcagccttc gtttaggtta cctctgctaa tatctttctc attgagatga aaattaaggt
     3661 aagcgaggaa acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt
     3721 tttatggccg tactgggaat aattttattt tctggttttc tggccgcgta tttcagccac
     3781 aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg
     3841 tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa
     3901 tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat
     3961 aacggatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact
     4021 cggttaatca cattttgttc atcaataaac atgcagcgat ttcttccggt ttgcttaccc
     4081 tcatacattg cccggtctgc tcttccaatg accacatcca taggctcttc aggaaatgcg
     4141 cgactgacac ctgctgtcac ggtaatgttg atatgccctt cagaatgcgt gatggcatgg
     4201 ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc attagccgct
     4261 ttgacaataa tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcacgcgtc
     4321 caactggcta agtaagttgc cagggtgcgt aatactacat cgccgattaa atgcccgtag
     4381 gtatcattaa ccaatttaaa tcggtcaata tccaacaaca ttaaataaag attcagaggc
     4441 tcagcgttgc gtaactgatg atcaaaggat tcatcaagaa cccgacgccc cggcaatccc
     4501 gtcaaaacat ccatattgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc
     4561 gcagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg ccagtgattt
     4621 tcaacaatag ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc
     4681 attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga
     4741 ccaaactggc acagtccata agaatggtta tccgttattt ctggcttact ggcatctctc
     4801 gcgaccacgc tgtgaaacat actcaccagc cactggtagt gggcatcgat agccttattg
     4861 agatttaaca agatggcatc aatttccgtt gttttcttga tcattgccac tcctttttca
     4921 cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata
     4981 gaaatccgaa gttatgagtc atctctgaga taacattg
//
LOCUS       DAZFVO020000247         4945 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFVO020000247 DAZFVO020000000
VERSION     DAZFVO020000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.249"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(326..1045)
                     /locus_tag="ACHKNB_RS23940"
                     /old_locus_tag="pgaptmp_004788"
     CDS             complement(326..1045)
                     /locus_tag="ACHKNB_RS23940"
                     /old_locus_tag="pgaptmp_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HGU4198158.1"
                     /translation="MDKLLNKKIKVKQSNELTEAAYYLSLKAKRVLWLCLMQTYFTAS
                     VSEDDDDMAVLGDSTFKVKVADYQQIFQVSRNQAIKDVKEGVFELSRSAVIFYPKEGR
                     FDCIARPWLTEAGSRSARGIWEIEFNHKLLPYIYGLTNQFTTYSLRDCGSLRNPRTIR
                     LYESLAQFKSSGIWVTTHAWLNDRFLLPESQQKNLAELKRSFLDPALKQINEKTPLLA
                     KYSIDDAGKFLFSIIDKQNPA"
     gene            1610..1939
                     /locus_tag="ACHKNB_RS23945"
                     /old_locus_tag="pgaptmp_004789"
     CDS             1610..1939
                     /locus_tag="ACHKNB_RS23945"
                     /old_locus_tag="pgaptmp_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrfB-related DNA-binding protein"
                     /protein_id="HGU4198159.1"
                     /translation="MAQKNRITEAEWTQLLPHMATLAHITTDIGYSVLVKGEKASDVA
                     ARVGRSKQNISSTAKRIWDLYQNTTLHTEDGEALQLVQVWIPEKLAKKVLAEAAKYSV
                     NKNIISE"
     gene            1962..2147
                     /locus_tag="ACHKNB_RS23950"
                     /old_locus_tag="pgaptmp_004790"
     CDS             1962..2147
                     /locus_tag="ACHKNB_RS23950"
                     /old_locus_tag="pgaptmp_004790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198160.1"
                     /translation="MQDKILLIIIVLGFLALIFFYSEGVIIDKIMNFYQNIGENKKAK
                     EALKRSIESDRKNNRLK"
     gene            complement(2172..2957)
                     /locus_tag="ACHKNB_RS23955"
                     /old_locus_tag="pgaptmp_004791"
     CDS             complement(2172..2957)
                     /locus_tag="ACHKNB_RS23955"
                     /old_locus_tag="pgaptmp_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HGU4198161.1"
                     /translation="MNLPTPETYDELQRAYDFFNEKLFSNELPPCLITLQREKRTYGY
                     CSFKRFVGRESGDTVDEIAMNPVYFSIRTIKATLSTLVHEMVHQWQFHFGEPGRRSYH
                     NKQWAARMERVGLMPSDTGEQGGRKVGQSMTHYIIAGGPFDMACDELLTGHFRLSWMD
                     RFPPYQPKPGAVLSPTGKGYVDDEEDDSEHELEEGRDPVELDDEIIEAMRFVAPPPEA
                     PVNKTNREKYSCPVCHINLWGKPGIVVYCGGEHCEKALLVVIK"
     gene            complement(3108..4373)
                     /gene="mucB"
                     /locus_tag="ACHKNB_RS23960"
                     /old_locus_tag="pgaptmp_004792"
     CDS             complement(3108..4373)
                     /gene="mucB"
                     /locus_tag="ACHKNB_RS23960"
                     /old_locus_tag="pgaptmp_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     MucB"
                     /protein_id="HGU4198162.1"
                     /translation="MFALIDVNGMYASCEQAFRPDLANRAVAVLSNNDGNIVARNYLA
                     KKAGLKMGDPYFKVRPIIERHNIAIFSSNYTLYASMSARFAAVVESLASHVEQYSIDE
                     LFVDCKGITAAMSLDAFGRQLREEVRRHTTLVCGVGIARTKTLAKLCNHAAKTWPATG
                     GVVALDDGARLKKLMSILPVAEVWGVGHRTEKALATMCIKTVLDLARADTRLIRKTFG
                     VVLERTVRELRGEACFSLEENPPAKQQIVVSRSFGQRVEALTDMQQAVTGFAARAAEK
                     LRNERQYCRVISVFIRTSPYSVRDTQYANQATEKLTVATQDSRTIIQAAQAALARIWR
                     EDIAYAKAGIMLADFSGKEAQLDLFDSATPSAGSEALMAVLDGINRRGKSQLFFAGQG
                     IDNSFAMRRQMLSPDYTTDWRSIPTATIK"
     gene            complement(4361..4801)
                     /gene="mucA"
                     /locus_tag="ACHKNB_RS23965"
                     /old_locus_tag="pgaptmp_004793"
     CDS             complement(4361..4801)
                     /gene="mucA"
                     /locus_tag="ACHKNB_RS23965"
                     /old_locus_tag="pgaptmp_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107685442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit MucA"
                     /protein_id="HGU4198163.1"
                     /translation="MKVDIFENSGASQIHSIPFYLQRISAGFPSPAQGYEKQELNLHE
                     YCVRHPSATYFLRVSGSSMEDGRIHDGDVLVVDRSLTASHGSIVVACIHNEFTVKRLL
                     LRPRPCLMPMNKDFPVYYIDPDNESVEIWGVVTHSLIEHPVCLR"
BASE COUNT         1244 a         1273 c         1181 g         1247 t
ORIGIN      
        1 cccaccttac cgggcgccgc tgccgcgtgg gaatccgccc accttaccgg gcgccgctgc
       61 cgcgtgggag tccgcccacc ttgccgggcg ccgctgccgc gtgggaatcc gcccaccttg
      121 ccggacgacg ctgccgcgtg ggaatccgtc caccttaccg ggcgccgctg ccgcgtggga
      181 atccgcccac cttgccgggc gccgctgccg cgtgggaatc cgcccacctt gccggacgac
      241 gctgccgcgt gggaatccgc ccaccttcct gaagaaaacc aaaaaaaagc ctgtcaaatg
      301 acaggctcat gagtgctggt ttatgtcagg cggggttttg cttatcgatg attgagaaca
      361 aaaattttcc tgcatcatca atgctgtatt tagcaagtaa gggggttttt tcgtttatct
      421 gtttgagtgc aggatcgaga aaagaacgtt tcagctcagc caggttcttc tgctgggatt
      481 caggcaaaag gaaacggtca tttaaccagg catgagtagt aacccatatg cctgaagatt
      541 tgaactgagc aagactttca taaaggcgga tcgtccgagg atttctaaga ctgccacaat
      601 cgcggagcga gtaggtagtg aactggttgg tcagtccgta aatgtacggc aggagtttat
      661 ggttaaattc gatttcccag ataccacggg cagatcggct gccagcctct gttagccagg
      721 gccgcgctat gcagtcaaag cgcccttctt tcgggtaaaa gatcactgct gaacggctta
      781 actcaaacac gccttcttta acatccttga tagcctggtt acggcttacc tgaaaaattt
      841 gctgataatc agccaccttt actttgaaag tagagtcacc gagtacagcc atatcatcat
      901 catcttcgct tactgaagct gtgaaatacg tctgcataag acataaccag agaacgcgtt
      961 ttgctttcag cgagaggtag taagcagctt cggtaagctc attagactgc ttaactttta
     1021 tttttttatt cagcaactta tccattcgcc ttgcctttgt gggctaaaaa ttattctcca
     1081 cattaccaca aagttccaac aagtaaatat attttttaca tttccgtcta aaggtgggcg
     1141 gattcccata actaggtggg ctaattccca taggaaggtg ggcggattcc cataactagg
     1201 tggggggaat cccataggaa ggtgggcgga ttcccagaga aacaccgttt ttacctttta
     1261 aaatcaattg gataaacata gctaaaagac tttaaaaaga taaaactcta taaaatactt
     1321 tctaaaagta cgcctgtgga tatgtgcata tcatggctta ccagcgccgc tacgcggctc
     1381 ggtcaacccc tcagaaccgc gaaaacaagt ttcgcagttc ttcacggttc atgctttaat
     1441 ccgtgcatgg cgccagtacc ttcgcggcac catcgaggcc cgcgctgctg ccgcaccggg
     1501 cctcaatcac tcaagcataa aataaagttt aaaataaccg tacttgtatt gacttaaagc
     1561 ctttaaacct ctaatctctc cgtatcagct tttttcggag acgaaccaga tggcgcagaa
     1621 aaacagaatt accgaagcag aatggacaca gttattgcca cacatggcta cgctcgcaca
     1681 catcacaacc gatattggtt attctgtttt ggttaagggt gaaaaagcat ccgatgttgc
     1741 cgcgcgtgta ggccgttcca aacaaaatat ttccagtaca gcaaaacgta tctgggatct
     1801 ttatcaaaac accacgttgc atactgaaga tggcgaagca cttcagctcg ttcaggtctg
     1861 gatacctgaa aaactggcta aaaaggttct agctgaagcg gctaagtaca gcgtaaataa
     1921 aaatattatc tctgaataga gagacataaa gcgggtggaa tatgcaagat aaaatcctat
     1981 taatcattat cgttctcgga tttctagcat taatattttt ttattctgaa ggcgtgataa
     2041 ttgataaaat aatgaatttt tatcaaaata taggggaaaa taaaaaagca aaggaagcat
     2101 tgaaacgcag catagaaagt gatcgcaaaa ataacagatt aaaatagaaa aataaaagcc
     2161 cttacgggct tctattttat cactaccagc agtgcctttt cgcaatgctc cccaccgcag
     2221 taaactacaa tacccggctt accccaaaga ttgatatggc ataccggaca gctgtacttt
     2281 tcccggttag ttttattcac tggagcctcc ggcgggggtg caacaaatcg catggcctct
     2341 atgatctcgt cgtcgagttc aaccgggtca cgcccttctt ccagttcatg ttcgctatca
     2401 tcttcctcgt cgtcaacata gccttttcct gtggggctta gcacagcacc aggcttaggc
     2461 tggtaaggcg gaaacctgtc catccaggaa agccggaaat gacctgtcag cagttcatca
     2521 caggccatat cgaaagggcc accggcaata atataatggg tcatgctctg gcccactttc
     2581 ctgccgccct gttcgccggt atccgaaggc attagtccta cccgttccat ccgggccgcc
     2641 cactgtttgt tgtgatagct acggcggcca ggctcgccaa aatggaattg ccactgatga
     2701 accatctcat gcaccagtgt tgaaagcgtg gcctttatgg ttctgatcga gaaatacacc
     2761 ggattcatag cgatctcgtc taccgtgtcc ccactctcac ggccgacgaa acgcttaaag
     2821 gaacaatagc catatgttcg cttctcacgc tgcaacgtta tcaggcatgg cggcagctcg
     2881 ttgctaaata gcttctcatt gaaaaaatcg taggctctct gaagttcatc gtaggtttcg
     2941 ggcgtaggca aattcactgt taacctcaca tatatgtatt gtgcaataca cataatttag
     3001 ctgatctttt atacagagga aacggctttt accgaaaacc gcgaaaccgt gccgaaactt
     3061 gtttcggccg gttgaggggt tgaccggccc gcgtgcggcg ccggtaatta ttttatggtg
     3121 gctgttggta tcgagcgcca gtctgtcgtg taatcaggtg acaacatctg acgccgcatg
     3181 gcaaaggagt tatcgatgcc ctggcctgca aaaaacagct ggctctttcc acgccggttt
     3241 ataccatcga gaacggccat taacgcctcg ctgccagctg aaggcgtagc agagtcgaat
     3301 aaatcaagct gggcctcctt accgctaaaa tctgccagca tgatccctgc ttttgcatac
     3361 gcaatatctt cccgccagat ccgcgccagc gcggcttgtg ctgcctgaat tatcgtgcgg
     3421 ctgtcctggg ttgcaaccgt cagtttttcg gttgcctgat tggcatactg tgtatcacgc
     3481 actgaataag gactggtgcg gataaagacg cttatgacgc ggcagtattg ccgctcatta
     3541 cgcagttttt cagctgcgcg cgctgcaaat ccggtgacag cctgctgcat gtccgtcagg
     3601 gcttctacgc gttggccgaa tgagcgcgac acaacaatct gctgcttcgc aggagggttt
     3661 tcttccaggc tgaagcaagc ctcgccgcgc agctcccgta ccgttctttc aagcacaacg
     3721 ccgaacgttt tacggattag gcgcgtatct gccctggcta aatccagcac cgttttgata
     3781 cacattgtgg cgagtgcttt ctctgtacga tggccgacgc cccagacttc cgcaaccggc
     3841 aggatgctca ttaatttttt cagtctggcg ccatcatcca gagcaaccac cccgccagtt
     3901 gcgggccatg tttttgcagc gtggttacac agtttcgcca gcgtcttagt gcgggcaata
     3961 ccgaccccgc ataccagcgt ggtgtgtcgc ctgacttcct cgcgcagctg gcgcccgaaa
     4021 gcgtcaagac tcatggcggc cgttatccct ttgcagtcaa caaaaagctc gtcgattgaa
     4081 tactgttcga cgtggcttgc aagggactca actacggccg cgaaccgggc cgacatggag
     4141 gcatagagag tgtaattaga gctaaaaata gcgatgttgt gacgctcaat tatgggtctg
     4201 actttgaagt acggatcgcc cattttcagg cccgctttct tcgccaggta attacgggcc
     4261 acaatgttgc cgtcattgtt ggataaaacg gccaccgctc ggtttgccag atcgggccta
     4321 aatgcctgct cacagctggc gtacatgcca tttacatcaa tcagcgcaaa cataccggat
     4381 gctcgataag ggaatgcgta accacacccc agatttcaac gctctcatta tccgggtcaa
     4441 tgtagtagac aggaaaatct ttgttcatcg gcatcaggca gggtctgggc ctcagcaata
     4501 gccgcttcac ggtaaattca ttatggatgc aggcaactac gattgagccg tggctggccg
     4561 tcagcgagcg atccacaacc agtacgtcac catcatggat gcgaccatct tccatcgacg
     4621 agccagatac ccgcaggaag taagttgctg aagggtgacg aacgcaatac tcatgcaggt
     4681 ttaactcctg cttttcatag ccctgggccg ggctggggaa tcccgcagaa attctttgca
     4741 gataaaacgg gatgctgtgt atctggctgg cgccggaatt ttcaaaaata tcgaccttca
     4801 tagcgatctt ccaaataact gtttatttat acagtatcgc tgttatggtc aggttggcaa
     4861 gttttaccgc ttcgctacta tatgtagtgc tgtttcagct gccggcgaac gctatatata
     4921 gtgtttgtcg gctttctgct tgctc
//
LOCUS       DAZFVO020000248         4940 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFVO020000248 DAZFVO020000000
VERSION     DAZFVO020000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.250"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2256
                     /locus_tag="ACHKNB_RS23970"
                     /old_locus_tag="pgaptmp_004794"
     CDS             <1..2256
                     /locus_tag="ACHKNB_RS23970"
                     /old_locus_tag="pgaptmp_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4198164.1"
                     /translation="VIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVG
                     KTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGF
                     GRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGIN
                     ALAGSVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIG
                     VMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSART
                     YENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQN
                     RSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAP
                     SGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICVPQGSYDIDDKV
                     GGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQ
                     AGFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDWEG
                     ISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFG
                     AGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDL
                     PQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARG
                     RTWIFGGDIRF"
     gene            2325..3500
                     /gene="senB"
                     /locus_tag="ACHKNB_RS23975"
                     /old_locus_tag="pgaptmp_004795"
     CDS             2325..3500
                     /gene="senB"
                     /locus_tag="ACHKNB_RS23975"
                     /old_locus_tag="pgaptmp_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HGU4198165.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(4130..4588)
                     /locus_tag="ACHKNB_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /pseudo
     CDS             complement(4130..4588)
                     /locus_tag="ACHKNB_RS23980"
                     /old_locus_tag="pgaptmp_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1370 a         1154 c         1181 g         1235 t
ORIGIN      
        1 gttataaaac tggctatcgg ctcaggcata ttattgctca gctgcggtgc ttactcacaa
       61 tccatcagtg aaaaaactaa ttccgacaaa aaaggagcgg cagaattcag tccgctcagc
      121 gtttctgtcg ggaagacgac cagtgagcag gaagctctcg agaaaacagg cgcgaccagt
      181 tcccggacaa cggacaaaaa cctgcaatca cttgacgcaa cagtgcgtag tatgcccggt
      241 acttatactc aaatagatcc tggtcaggga gcaatcagtg tgaatattcg aggcatgagc
      301 ggatttggtc gtgtaaacac tatggtcgat ggtattaccc agagttttta cggaacctct
      361 acctccggaa caacgacgca tggttcaact aacaatatgg ctggcgtact tatagatcct
      421 aacttactgg tagcagttga tgttacacgc ggtgacagca gtggctctga agggatcaac
      481 gcccttgccg gtagtgtaaa tatgcgtact attggcgttg acgatgtaat atttaacggt
      541 aatacatatg gccttcgttc acgtttctct gtcggtagta atgggctggg acgcagcgga
      601 atgatcgccc ttggtggaaa aagcgacgct tttacggata cgggaagcat tggcgttatg
      661 gctgctgtga gcggcagttc tgtgtactct aatttctcaa atggttctgg aattaacagc
      721 aaagagtttg gttatgataa atatatgaag cagaacccca aatcccaact gtataaaatg
      781 gatatcagac cagacgaatt taacagcttc gaactttccg ctcgaaccta tgaaaataaa
      841 tttacacgtc gtgatataac cagtgacgac tattacatta aatatcatta cacccctttt
      901 tctgaattaa ttgactttaa cgtaacggcc agtaccagtc gcggtaatca aaagtatcgt
      961 gatggctcgc tgtatacttt ctacaaaacc tcagcgcaaa atcgttctga cgcgctggat
     1021 atcaacaata ccagccggtt cactgtcgcg gacaatgacc tggagtttat gctgggcagc
     1081 aaactgatgc gtacccgcta tgaccggacc attcactcag cggcgggcga cccgaaagcg
     1141 aatcaggaat cgatcgagaa caatccgttc gcaccctccg gccagcagga tatttcagcg
     1201 ctgtataccg ggctgaaggt tacgcgcggc atctgggagg cagatttcaa tctcaactac
     1261 acacgtaaca ggatcacagg gcacaagccc gcctgcgatt cacgcgttat ctgcgtgcca
     1321 cagggtagct acgatattga cgataaagtg ggtggcttca acccttcagt tcagctttct
     1381 gctcaggtaa caccatggct tcagccgttc attggctaca gcaaatccat gcgcgccccg
     1441 aacatccagg agatgttctt ctctaattca ggaggcgcat ccatgaaccc attcctgaag
     1501 cctgaacgtg cagaaacctg gcaggcgggt tttaacattg ataccagaga tttagtggtc
     1561 gaacaggatg ccctgcgctt taaggctctg gcgtaccgca gcaggatcca gaactacatc
     1621 tacagcgagt cttatctggt ttgttctgga ggtcgtaaat gcagtatggc tgaggtgatt
     1681 ggcaatgact gggagggcat tagcgatgaa tacagcgaca atatgtacat ctacgttaac
     1741 tcggcaagcg acgttatcgc aaagggcttc gaactggaga tggattatga tgcaggtttt
     1801 gcttttggcc gactctcttt cagccagcag caaacagacc agccaacctc catcgccagc
     1861 acctactttg gcgcagggga tatgaccgaa ctgcccagaa aatacatgac gctggatact
     1921 ggtgttcgct tcttcgataa cgcgttgacc ctgggcacta tcataaaata cacaggcaag
     1981 gctcgtcgcc tgtcgcctga ttttgagcag gacgaacata ccggcgcaat aatcaaacag
     2041 gatttgccgc agatcccaac gattatcgat ctctatggta cttacgagta caaccgcaac
     2101 ctgacactga aactttcggt acaaaacctg atgaacagag attattcgga ggcgctgaat
     2161 aagctcaaca tgatgccagg tcttggtgac gagacccacc cagccaattc cgcgcgtggc
     2221 agaacatgga tatttggcgg ggacattcgt ttctgatgac aatttccgct aggttcagac
     2281 aatacgtttt ctctcttatg tcaattttat tgcaggaacg aaaaatgaat attttcactt
     2341 tatccaaaac accgctatac ctgttaattt cactattttt acccacgatg gccatggcta
     2401 tcgatccacc tgaacgcgaa ctttcgcgat ttgccctgaa aacgaattac cttcagtccc
     2461 ctgatgaagg cgtctatgaa ctggcgtttg ataatgccag taaaaaggtg tttgcagcag
     2521 tcaccgatcg tgtaaatcgt gaagccaata aaggctatct gtattcgttt aattcagatt
     2581 cgctgaaagt cgaaaataaa tacacgatgc cataccgggc attttcgctg gcgataaatc
     2641 aggataaaca tcagctctat atcggacaca cccagtcagc gtccctgcgt atcagtatgt
     2701 ttgacacccc aaccggcaaa ctggtaagaa ccagcgacag gttaagtttt aaagcggcaa
     2761 acgctgcaga ttcgcgtttt gagcatttgc gccatatggt ttacagccag gattccgata
     2821 ccctgtttgt gagttatagc aatatgctga aaacggccga gggcatgaag cctctgcata
     2881 agctgttaat gctcgacggg acgacgcttg ccttaaaagg cgaggttaag gatgcttaca
     2941 aaggtacagc gtatggtctg acgatggatg aaaaaacaca gaaaatctac gttggcggaa
     3001 gagattacat caacgaaatt gatgcgaaaa atcagacgct gctgcgtacc atcccgttga
     3061 aagatccgag accacaaatc acaagtgtgc agaatctggc ggtggactcc gcttctgacc
     3121 gtgcctttgt ggtggtattc gaccatgacg atcgttccgg tacaaaagat ggactctata
     3181 tttttgactt acgcgacggt aaacagcttg gctatgtgca cacaggagcc ggagctaacg
     3241 cggtgaaata caatccgaaa tataacgaac tgtatgtcac caacttcact agcggcacca
     3301 tcagcgtagt ggatgccacc aaatacagca tcacccgtga atttaacatg ccggtctacc
     3361 caaaccagat ggtgttgtcg ggtgatatgg atacccttta cattggcatc aaagaaggct
     3421 ttaaccgcga ttgggatcct gatgtgtttg tggaaggagc taaagaacgt attctgagca
     3481 ttgatttgaa aaagtcgtga tcgggggaaa acatgggata aaaactccgt tattccacta
     3541 aactggccgg actaggaaaa gcaaacatct ctgtcctgcc ggtacgcctg ttacgccgct
     3601 ccatgaggct gaggaatctc cagaaaacga gacaggcaca gcattgtacg atcaactgat
     3661 ttgtgccaat aattcaatga aatcacagct atgcctgttc atggggttct aggccggaac
     3721 cccagatttt tccgattgtt tgttttctaa ctgattattc agtagtttta gagggctgac
     3781 gctcagtgga acgaaaactc acgttaagag gttttgacta tgataaaagt caaagtaggg
     3841 gtgagtttgg atatggaaat tattgtacta taagggcttc cccgaacagt caacgtcggt
     3901 atttatggtt tgtctgcact acagttaaaa ataaagggga gagactgcgg tactataaac
     3961 ggtcataata aatcgttatc gattcggacc ctgatgaaag acgcgcgtga ggctcttgtt
     4021 cgttaagcgg atacgtatat cctgtacaat aatcagatat acctcacggg gtctaaccct
     4081 ttatcatcaa cgagttgcag gtcagatagt catacggttg ctgcgtgctt tagctggctg
     4141 aatacagtct gaaatcatca ccgtaatgca atgatgccca gcacagcctt gcatttttgg
     4201 cggcgatggc cacaacagcg cgccagtatc ctctgcgttc gaccagtgaa cagacccaac
     4261 gactgaatga gtcagtcctt ttctcagcgc caatcagtac tgaacgagca ccctgaacca
     4321 gaagcgttcg cagatacgaa tcaccggctt ttgttatcct gcctagcttc gactttccgc
     4381 cgctgctgta ttgtgatgga gtcagtccca gccaggccgc cagttgccgt ccatttttaa
     4441 aatcatgtgc attaccgata caggcaacca gcgcacaggc agttgtggga ccaattcctt
     4501 ttagctccat cagccgctga ctgcggtgat ctgttctggc catacgggac aaaattttgt
     4561 catattcagt gatgttagct tcaatgcggg gtcagtttgg agatcgtaaa attttgttcg
     4621 ttaagcacat tatttaacca atagatatgc gtaacggatt gattttgcgt caacggctat
     4681 cgcttttaag ttatcgccat tgaggtattg catttaggtt atccgataga ggtcaaatcc
     4741 tctgaaaacg ccgtatagca cgggagtaca ccctaccgca atagcgcaac cgtacaatat
     4801 tgtacgtttc ccaaacgcag cctaggaagc acgtcgtcag tattggagaa aacttacaaa
     4861 cgctcgccgc agccccagcg accgaacgtt agtgagcgag tgggcgagga agcggaaaag
     4921 tgtcaggtct gaattgagca
//
LOCUS       DAZFVO020000249         4929 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFVO020000249 DAZFVO020000000
VERSION     DAZFVO020000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.251"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(293..1324)
                     /gene="rsmC"
                     /locus_tag="ACHKNB_RS23985"
                     /old_locus_tag="pgaptmp_004797"
     CDS             complement(293..1324)
                     /gene="rsmC"
                     /locus_tag="ACHKNB_RS23985"
                     /old_locus_tag="pgaptmp_004797"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HGU4198166.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1427..1840
                     /gene="holD"
                     /locus_tag="ACHKNB_RS23990"
                     /old_locus_tag="pgaptmp_004798"
     CDS             1427..1840
                     /gene="holD"
                     /locus_tag="ACHKNB_RS23990"
                     /old_locus_tag="pgaptmp_004798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HGU4198167.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1809..2255
                     /gene="rimI"
                     /locus_tag="ACHKNB_RS23995"
                     /old_locus_tag="pgaptmp_004799"
     CDS             1809..2255
                     /gene="rimI"
                     /locus_tag="ACHKNB_RS23995"
                     /old_locus_tag="pgaptmp_004799"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGU4198168.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2270..2947
                     /gene="yjjG"
                     /locus_tag="ACHKNB_RS24000"
                     /old_locus_tag="pgaptmp_004800"
     CDS             2270..2947
                     /gene="yjjG"
                     /locus_tag="ACHKNB_RS24000"
                     /old_locus_tag="pgaptmp_004800"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HGU4198169.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3333..4568
                     /locus_tag="ACHKNB_RS24005"
                     /old_locus_tag="pgaptmp_004801"
     CDS             3333..4568
                     /locus_tag="ACHKNB_RS24005"
                     /old_locus_tag="pgaptmp_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4198170.1"
                     /translation="MALSDAWLRSVVGKKRDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELELNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
BASE COUNT         1250 a         1175 c         1256 g         1248 t
ORIGIN      
        1 cgtctaccaa ttccgccacc ttcgcatacc atcaattctt aaaaagaatt gctaccacgg
       61 aggcgcattc tagtggtttt cagcttttcg tcaatagtta attatcgccg gaggtgtaat
      121 tgctggaaaa atgtccatca ggaaactagc gtgcaggttt ggtaggcatg cgggggcaga
      181 tgccagatgc gacgctggcg cgtcttatct ggcctacgaa gggctaacgt gcaggttttg
      241 taggtcggat aaggcgttca cgccgcatcc gacacggtat tcggcgagat aattaacctt
      301 tcttcgcctg gcgggtcata atggcgcgat acaccttgaa acgcccggtt tgcgcaatca
      361 cttcatggaa gccaaatgtc tcatccagca cgtccgggta aggcaggaag gcgttcgcta
      421 caattcgcag ctcgccgccg ctattaagat gacgcaccgc gccgcgaatc agcgtttgcg
      481 ccgcatccag gctggtttgc atcccatcgt ggaacggcgg gttggagatg atcatatcaa
      541 aacgaccttt cacctcggaa aagacgttgc tggcaaagac ttcaccttca acaccgttgg
      601 ccgcaagtgt tgcgcggctg gcttctaccg ctggcgcaga gacatcgcac aaggtgagac
      661 gaattttcgg cgaatggcgc gcaaaggcaa ctgaaagcac acccgcgcca cagccgacat
      721 ccagcacttt acctttcgtg tgcggggtta acgtcgagag cagcaactgg ctaccgacat
      781 ccagaccgtc gcggctaaac acgccaggca gcgttttgac cgtcaggcca tcgacgctgt
      841 attcgcccca gaatttatcg gcatcaaata ccggctgttt ttccagacga ccaaaataga
      901 ggccacagcg acgagcgctg tcgactttat tcaacggcgc ataatctgcc agcatctgct
      961 cggcgctgcg cacgccgctg cggttctcgc caacgacaaa aatatctgtc cccactggca
     1021 gcagagaaag taaattcatc aactggaact gggcttccgg tttgttcttc ggccagtagt
     1081 aaatcagcgt atcgcaatct gcgacgtcat ccaccgtggc gaccagactg aaacgggcgt
     1141 tatcccccat ctggcggctt aatacctgcc agtggtggaa ttgctgggta tgagcacggc
     1201 tggccgcggt atctaaacgc gcgggcaggt catcctgtaa gtctccggca aacagaatac
     1261 ggctttgttc gaaatcatca ctgtgacgca gcaagacttc acttgccggg gtaaatgcag
     1321 acatggaatg ctcctcaatt gatactggcg gcgattatag ccatatgttg gcgcggtatc
     1381 gacgaatttg ctatatttgc gcccctgaca acaggagcga ttcgctatga catcccgacg
     1441 agactggcag ttacagcaac tgggcatcac ccagtggtcg ctgcgtcgcc ctggcgcgtt
     1501 gcagggggag attgccattg cgatcccggc acacgtccgt ctggtgatgg tggcaaacga
     1561 tcttcccgcc ctgaccgatc ctttagtgag cgatgttctg cgcgcattaa ccgtcagccc
     1621 cgatcaagtg ctgcaactga cgccagaaaa aatcgcgatg ctgccgcaag gcagtcgctg
     1681 caacagctgg cggttgggta ctgacgaacc gctatcactg gaaggcgctc aggtggcatc
     1741 accggcgctc accgaattac gggcaaaccc aacggcacgc gccgcgttat ggcaacagat
     1801 ttgcacatat gaacacgatt tcttccctcg aaacgactga tttaccagcg gcttaccaca
     1861 ttgaacaacg cgcccacgcc tttccgtgga gtgaaaaaac ctttgccagc aaccagggcg
     1921 agcgttatct caactttcag ttaacgcaaa acggcaaaat ggcggcgttt gcgattacgc
     1981 aagtggtgct ggatgaagct acattgttca atattgcggt cgatcctgac tatcagcgtc
     2041 agggattggg aagggtgctg ctggaacatc tgatcgacga actggaaaaa cgcggcgtgg
     2101 cgacactatg gctggaagtc cgtgcttcaa acgctgccgc cattgccctg tacgaaagtt
     2161 taggctttaa cgaggcgacg attcgccgca attactaccc caccacggac ggtcgcgaag
     2221 acgccatcat catggcgttg ccaatcagta tgtaagacaa ggtggaataa tgaagtggga
     2281 ctggattttc tttgatgccg atgaaacgct gtttaccttt gactcattca ccggcctgca
     2341 gcggatgttt cttgattaca gcgtcacctt taccgctgaa gattttcagg actatcaggc
     2401 cgttaacaag ccactgtggg tggattatca aaacggcgcg atcacttcat tacagcttca
     2461 gcacgggcgg tttgagagct gggccgaacg gctgaaagtt gaagcaggct tgcttaacga
     2521 tgcctttatt aatgcgatgg cggaaatctg tacgccgttg ccgggcgcgg tttctctgct
     2581 taacgccatt cgtggcaacg ctaaaatcgg catcatcacc aacggtttta gcgccttgca
     2641 gcaagtgcgt ctggaacgga cgggcctgcg tgattacttc gatttgctgg tgatttccga
     2701 agaagttggc gttgctaaac cgaataagaa aattttcgat tatgcgctgg aacaggcggg
     2761 caatcctgac cgttcacgcg tgctgatggt tggcgacact gccgagtccg atattctcgg
     2821 tggcatcaac gccgggcttg cgacctgctg gctgaatgca caccatcgcg agcaaccaga
     2881 aggcatcgcg cccacctgga ccgtttcttc gttgcatgaa ctggagcagc tcctgtgtaa
     2941 acactgattg cctccccccc gttgatgggt aaaatagccg caatttttcg ttttcaacaa
     3001 gcgcggcgcg atgccgctta ctcaagaaga aagaattatg acgttgtctc cttatttgca
     3061 agaggtggcg aagcgccgca cttttgccat tatttctcac cccaataatt aagcctaaat
     3121 taaagctctt ttactttttc aaagtccttt cagttaattg atgttttgtt ttataagtca
     3181 tttaaataca gttagttatg tggcttcttt aattgaaaat tctttcatgt aactttaaat
     3241 cgatcaggtt gctttcatca aaaatctgta catatgcttg tacataatgt acaaagcaac
     3301 agaggtgttt tgcgatttgt acaaggtgag taatggcgct gtctgatgcg tggttgcgtt
     3361 cagtcgttgg aaagaaacgt gataaggttt tggttaaatc cgatcgtgat ggtctgtctg
     3421 tcagagtatc accgaaaggt cgcgtagtgt tccaatatcg ttatcaatgg gcagggaaag
     3481 gtgagcgcct tgatatcgga acttacccgg caactggatt aaaagaggcc agagaagaag
     3541 ttatccgtct ccgtggtgaa ctcgagttaa accgcaatcc acgattagtc aagcaggctg
     3601 aaaaacgaaa agctactgaa gccatgacgg tagagtctgt gatccgtgcc tggtatgaag
     3661 catattgtgt aaaaaataaa aaaggttctg aacagatact ccgctcgttt gagctgcacc
     3721 tgttctctaa aatcgggaat atccctcacg atgcagctac attgcatgat tggttagaag
     3781 tcctggagcc tcttagcacc aagactccag caatagtaga ccgattgcta attaacgcaa
     3841 agcaggctca tgtctgggcg tataagagaa agctcattga aactcgccca ctgtcggata
     3901 tcacgggtaa agatatggat atccgtaaag gtcagaaaaa acggtttctg acacatgatg
     3961 aaattaacat cctttatgct gcgatcgatg gttctcgaat ggttcctaaa taccgagcct
     4021 tcattaaact attgctgcat tttggttgcc gtagttcaga gctaattact gctagggtgg
     4081 atgattttga tttcataaat aaggtatgga ctgtaccacc agaacgacat aagactgggg
     4141 acataacagg cgaaccgcta aagcggccca ttattgaacc ggttgaagag cttataaaat
     4201 acgttatctc tatgaacaac ggttccgata tgctttttac caaggaagga agcagggagc
     4261 cagttggtcg gacatcattg cagtcgctgc cttacaattt aatgcagttc gcatggcgac
     4321 gtttggggta tcaatttcct cattggtctc ttcatgattt gagacgaaca gcacgaacaa
     4381 acttttctga tcttactgct cctcatattg ctgaaataat gctcggtcat aaactgccag
     4441 gtgtatggca agtttatgat aagagcgatt atctagaaga acagcgtaaa gcttactggg
     4501 catggtggga gagggttgaa tcgatcgtta cttgtactga cttaatctcc aacggacagt
     4561 ttgcgtagcc agagcgtaat catatctgcc agtccgctgc ggataccttg tgtactgggt
     4621 ttttagccag actacgttaa ccggtagctt cagtatttgg ggggtcggca ggagtttgat
     4681 ggtttattgg gttactgtgg gactaaggat gtagggatgc caatcaatga attatgaaaa
     4741 atatcatagc acaaacagaa gcaagggttt gcgaaccttc tgctacttaa tttattatgt
     4801 gcaggtgatt tttaatgtga tgaagtgttt tatgactcgc ggaaactttg attttataaa
     4861 ctctaacgtg gttttctcag aagccgataa gtcattgtgg attgatctca ttaacaccga
     4921 ttgtatttg
//
LOCUS       DAZFVO020000250         4923 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFVO020000250 DAZFVO020000000
VERSION     DAZFVO020000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.252"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            219..1193
                     /gene="hemB"
                     /locus_tag="ACHKNB_RS24010"
                     /old_locus_tag="pgaptmp_004802"
     CDS             219..1193
                     /gene="hemB"
                     /locus_tag="ACHKNB_RS24010"
                     /old_locus_tag="pgaptmp_004802"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HGU4198171.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1299..2150)
                     /gene="tauD"
                     /locus_tag="ACHKNB_RS24015"
                     /old_locus_tag="pgaptmp_004803"
     CDS             complement(1299..2150)
                     /gene="tauD"
                     /locus_tag="ACHKNB_RS24015"
                     /old_locus_tag="pgaptmp_004803"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HGU4198172.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2147..2974)
                     /gene="tauC"
                     /locus_tag="ACHKNB_RS24020"
                     /old_locus_tag="pgaptmp_004804"
     CDS             complement(2147..2974)
                     /gene="tauC"
                     /locus_tag="ACHKNB_RS24020"
                     /old_locus_tag="pgaptmp_004804"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HGU4198173.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPLQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2971..3738)
                     /gene="tauB"
                     /locus_tag="ACHKNB_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /pseudo
     CDS             complement(2971..3738)
                     /gene="tauB"
                     /locus_tag="ACHKNB_RS24025"
                     /old_locus_tag="pgaptmp_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
     gene            complement(3751..4713)
                     /gene="tauA"
                     /locus_tag="ACHKNB_RS24030"
                     /old_locus_tag="pgaptmp_004806"
     CDS             complement(3751..4713)
                     /gene="tauA"
                     /locus_tag="ACHKNB_RS24030"
                     /old_locus_tag="pgaptmp_004806"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4198174.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1161 a         1410 c         1242 g         1110 t
ORIGIN      
        1 tagcagcaca accaaaggcc aaaaaataat aaccaagagc tataaataaa gattaaaaat
       61 attttcagtg aatatgaaat tattgctatt tctattacaa agatgtcctg gtctctcttt
      121 cattctcttc ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt
      181 atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc
      241 ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc
      301 ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg
      361 aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca
      421 tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa
      481 ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc
      541 agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg
      601 gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag
      661 gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga
      721 tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg
      781 cgattatgtc gtattcgacc aagttcgcct cttcctttta tggtccgttc cgtgaagctg
      841 ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg
      901 aggcgattcg tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac
      961 ctgctggagc atacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg
     1021 gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta
     1081 tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc
     1141 tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt
     1201 cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc
     1261 ctctccccaa gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt
     1321 ttatccccaa gaatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg
     1381 gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa
     1441 cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc
     1501 tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt
     1561 ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc
     1621 gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat
     1681 ttacggaaat catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc
     1741 gcctcatagg ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaattct
     1801 ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag
     1861 ttgtcgttat ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct
     1921 tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt
     1981 tgctgctgcg gcgtaatagt ttgatcgcgc agaaacacca cctgatggcg cagcaccgca
     2041 tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt
     2101 tgtgcgccga tatacggccc cagcggggta atgctcagac gttcactcat tgtacttctc
     2161 catgccaggg cgtcagacgg cgctgtaacg cgcgcaaacc cagttctaaa agaaaggcga
     2221 taatcgcaat caccgcgatc cccgccagca ccacgtcagt cgcgagaaat tcacccgctg
     2281 actgaaccat aaatcctaaa ccgcgcgtcg cggcaatcag ctccgccgct accagcgtag
     2341 accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca
     2401 aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc
     2461 gaacctgctg cgcgcttttc acccccgcca gcgccgacat cgccaccggt gcaaaaatcg
     2521 ctaaatagat cagtaagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg
     2581 gcaaataagc cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc
     2641 gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga
     2701 gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca
     2761 taaatccctg cggtccggca atggtgagca gttttgccag tacttgttgc agcggcggca
     2821 aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg
     2881 ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc
     2941 gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac
     3001 gcggcttaaa acatattcgc gcatggcgat gaattatgga tcggacttga tgctgcggct
     3061 cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc
     3121 agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg
     3181 ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat
     3241 ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg
     3301 attcgccgcc agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca
     3361 gatatagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc
     3421 cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac
     3481 attgcgccac ggtagtagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc
     3541 ctcaatacgc ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat
     3601 cagattcagc aacgtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc
     3661 gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat cggcatagag
     3721 atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg
     3781 gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc
     3841 gatgattgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata
     3901 ggtatttccc ttcaccagcc ccggaacgtc accttcaggc acgccgctta aacgcgccag
     3961 tttgctgatg ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg
     4021 agcatcgatg gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc
     4081 tttgcgcacc acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc
     4141 ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc
     4201 atcaatatct ccccgctgcc aagcagcgat aatcgcgggc ggctgcaggt tcacaatctc
     4261 cacttgcccg ggtttaatgc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt
     4321 agagataaac ggtacggcta tgcgtttgcc aatcagatct tccggtttgc tgatagtttt
     4381 ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaaa cttcaatcgg
     4441 cacctgttgg ctggctgcaa ccgctaacgg gctggaaccg aggttgccga tttgcacatc
     4501 gccagaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacggt
     4561 tgctccgctt tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga
     4621 ggtttgatac gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc
     4681 ggcaagaagt gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg
     4741 ggagcagtat tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga
     4801 ttttattctt agcaaaaaaa cggctaagga aagattaaat atgcaattac tccgttctaa
     4861 caattgggaa aattactaca cttcatttta gtgatgcata aacaaccaca ttacgagatt
     4921 tat
//
LOCUS       DAZFVO020000251         4888 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFVO020000251 DAZFVO020000000
VERSION     DAZFVO020000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.253"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..612)
                     /locus_tag="ACHKNB_RS24035"
                     /old_locus_tag="pgaptmp_004807"
     CDS             complement(<1..612)
                     /locus_tag="ACHKNB_RS24035"
                     /old_locus_tag="pgaptmp_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198175.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVL"
     gene            complement(770..2914)
                     /locus_tag="ACHKNB_RS24040"
                     /old_locus_tag="pgaptmp_004808"
     CDS             complement(770..2914)
                     /locus_tag="ACHKNB_RS24040"
                     /old_locus_tag="pgaptmp_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4198176.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            complement(2914..4620)
                     /locus_tag="ACHKNB_RS24045"
                     /old_locus_tag="pgaptmp_004809"
     CDS             complement(2914..4620)
                     /locus_tag="ACHKNB_RS24045"
                     /old_locus_tag="pgaptmp_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4198177.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFEHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLSLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGRKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADIAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            complement(4601..>4888)
                     /locus_tag="ACHKNB_RS24050"
                     /old_locus_tag="pgaptmp_004810"
     CDS             complement(4601..>4888)
                     /locus_tag="ACHKNB_RS24050"
                     /old_locus_tag="pgaptmp_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4198178.1"
                     /translation="VALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRAD
                     RERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
BASE COUNT         1087 a         1381 c         1135 g         1285 t
ORIGIN      
        1 cagcaccggc acggcattca tcgcatcaag aaccggtgat aaatcctcac caccattacc
       61 ttctgcctgc tgtgttgatt gctgaacacg ggactggaga tagttatttt tacggatgag
      121 cgaagccacc gcgtcaccaa tttccggata catctccctg atacgggcaa tctgctcatc
      181 agaaattttt tcgttttccg gtaacggtgt gggcttcata ccggcctggt ggatctgaga
      241 cgtcagtagt tccaccctgc gtttttcttc agctatctgc ccacgaagaa gtgcggcttc
      301 ctgttcggcc cgttgcttac cggaacgttc agcctcaagg acttcatagg gaatgacgtg
      361 tttaccgtcg cgggtgagca cccccttcgc ttccggctcc ttcacgtcct gcgtctgctc
      421 cacactggca tccggcgtcg gtgccacatt gttatcgccc gtctgagtct gtgcttcctc
      481 atccgcatgt ttttccgtgg tatcttccgt cacgacgtcc tgtgcgtgac tgtcaatatc
      541 cacatcccca agtccttcca gcattttttc cagttgttcc ggggtttctt cacccgtaaa
      601 ttcaaaatcc ataaataact ccgcatggtc tgtttatcgg acagatccga atggttgagt
      661 aaataaggct tatcgctgcc cccgcgaata agcgcaccgc tcccggaacg cttacctccg
      721 gaaacaaaaa accccgtacg atgacggggt tcagttgaag ccagagtttt cagagcgaca
      781 tttcattcat ccgctgttgt aacgtataca gcatctgttg ctgaagaacg tcctgctcct
      841 gttccatatt ctgtacgccg gtaatgattt ctgccgtatg tgcctggtta agcgcatcca
      901 cataacgctg cccctgtgtc agggcgactt cccgctgtgc actggcatta tcccgttgtg
      961 cagctgcatg tgccctggcg gcgtcagctt ccagttttgc cactctgcca gccatctcgc
     1021 gcatctggag ttctgcctgt tgttgctgaa gtgcctgttg ttgtgccgct acttcctgtt
     1081 cttccggcgt catttcatcc ggtgattttg gcgtccccag cgcagcacga atacgctcaa
     1141 caaactcctg tttctgcggc acatccagaa gattaaccca caggtcgagc acaacagcct
     1201 gcacctgagg cggcagcccc tgaataacct ctgacattct ctgtgcaagc tgtgccttaa
     1261 acgccggtgt ctgctgaaca ggcgccagcg caatatgtgt atttaacctt gaaatatcat
     1321 tggtcagttc accattatca ccttcagcat tgaggacaat ggtctggcga cgctggcgat
     1381 catcgcgatt aatcaccact gcatgattac ggcgtttttt caggtcatcg agaagataag
     1441 ccagcaacag tcttcccacc tgctggcagg caaactggta gttatcgttg atttccgcaa
     1501 gggttgtggc cccctgctcc accaggttac tgatagccac gcctgacgtc gcacctgaat
     1561 cctgcccgag aaatgcggaa tacactccca tggtatcctg gataagtttt tccgattcct
     1621 gcatgacctg aaactgctgg ctggcaacct gaaaatcctg ctcaacccga aaaacatctg
     1681 cgacactttt ctgatttttt cgggccggat tcagtttaat aatgccatcc ggacgttcga
     1741 tctgctccat caggtcgctg tctgacaact gggtggcatc ctcgtccata atcacgcgtt
     1801 tggcctgaag cagccaggtc agcttgatac gacgaaaatt cacctcatcc tgtgccggaa
     1861 tggcgcggga aattaacccg tatggctccc cggttttatc ctttcggtat ccccagaaag
     1921 gaaccagcgg aaacatcccc tgcggtgcac tacaggggcg atccacaata aagtgtggcc
     1981 cgacaaacca ggcttcacga atacggctta cccgcccgac tttcacctga acccgcccgg
     2041 atgccacagc taccgcctgc atcagattat ttttatcaaa ggccaccacc cgtccattac
     2101 tgagttcaat caccggaaga cgctcgaatg tacggtaata aaccacctga agcagcacac
     2161 gacggcgttc acgctgaagc cattcgttct gctgtcgatc ccatgactga tactcttccc
     2221 atgcactcat caacggactg ggctggcctt cagtaaccgt ggtatcgaca aaaccacgcc
     2281 agtcatcaat ggcataatcg ataacctgag ccattcccgg gaatgtagct tttgcctcat
     2341 cggtatccat ccagcggcga cgcatcagcc agcggcagtc acttaaatca gcctcccggc
     2401 tcagccagtc ccagaaaacc tcattccggc tgacagtaga caccttaaat tcaggcccga
     2461 acggatcgct gtttcgtctg acctccaccc aactgaggcc cgccttgatt tgttccgcat
     2521 aggcatcaga gcgggcttta ttcatattgc caaggcggca tgcatcggca aattcagcat
     2581 taatagcttc agccagtttt tcagtttcat catctggctc gtctgacatc accaccagat
     2641 cagtccgtgt tttggcctcc attcccagaa cgccatcgac ggtaggcgcg atgaggttat
     2701 ggatagtcat cggctgaccg cgatctttca gaacctgaag aacttccggt ggcaactgat
     2761 cgccatcgta atacgcacag gccttgtttg cggcatcacg ccatttaggc tggctgtcaa
     2821 tatcagaaca aagcgcctgt aactggcgct gagaaaaacg cggcgtggct ccattgtcgt
     2881 ttttcgtcgc catggtgtta gtttcatttt tcatcagtga gccatccagt gtgtagttct
     2941 gcgtttatcc gttttctgtt ttaccctcac aggcattctg gcgcgcatct cctgggcaat
     3001 catgtagctc atgagttgat catcaaaaca gccttcctgt gcattcatgg agcctttcgc
     3061 gtcataaacg taggtgttca tttccgataa tgtgcctgac cagcggatcc ctgatattcc
     3121 attattcagg agcgttttca ttccttcggt cagaacaggt ttgctctgac gggttgtcag
     3181 ccagccaagg cggggcgtat cgtcatcata tgcctgatca aggtgctgtt cgttgtagat
     3241 ataacgcgtc ggatagagct ccctgagttt cagaataacg gcatgtccgt gattgttacg
     3301 ctccggcccc acaaacgcgt tgttgtacat acgacagacc tgcgcaatga gatgagcaaa
     3361 aagctccgca tcaagatgac caaaccagtg ggccacctgc tcaccattac tgcatctgat
     3421 gatatccagc gatgagcggt ccccgtgctc aagcccctca gcaatatccg ctccgcagac
     3481 atactcttca tctggatccg gtagctccca gaccagcaga taattcatca gcgtccggtg
     3541 ctgttcggct ttattcccgt cacgcagaga ctgcgcttta gtcttccttc ctgtaacagg
     3601 ttcaatgtca taaacaatca gcggtggcga acagaatgat tctgcctgca acgtactttc
     3661 ggcactgaac acacgtcgtc cggacgtcag aaacgcctcc tgtggcgttg agggaaactc
     3721 ctgcttcatt tcctcacgct gttcagtttc cttattgatg taccactgct tctgttcatc
     3781 agtaagcgtg atgttcattg ccttctcaac cgcagaaaaa tacgtcattt tttcccgtga
     3841 cagcttcagc ccgctttccg gcactcgggc gctgtattta ggatcctgcc accaggcgta
     3901 aaaatggaat ttataatcct gtgccgtcaa cgataagcct gatgctgtga tctcctgtgc
     3961 tcggttactc atctcgtaaa aatcaccacc cacgccttca gctgtggact catcaaaaat
     4021 aatgcattca tcagagacgg cattaagcgt accggttcgc agttctttcg ccttagccgg
     4081 atatttcgcg caaattttgc cgtgctctga gatatgcagg cgctgcaccg tacctgaacg
     4141 gaatgaggtt gccacctgga tactcgagcc gtgaccaaac aggatatagc caccgctggc
     4201 accgctacga cgttcaacga tggtgaatga ggctctcagc cagtcaggga gatgctcaaa
     4261 cggtacagca atttttgtgc ggaaaatttc actggcagcc tgtttatcct gagcgacgat
     4321 cccgcatttg agatgcggaa tgaataatgc ctggtcgaga agataaatat caatggctgt
     4381 ggaaaatccc agctggcgcg ctttcaggat aatgttttta ttatgcatgt tacgaaacaa
     4441 ctggcgctgc gccggtcgca ttctgaaggt gaccagttca cctttttcgt tctgtatttt
     4501 gtagagatga ttgagccgcc accagggatt gctcagttta gtcatgatga acagacgttg
     4561 ttcagcctcg gtcatttctg acggctcatc acatcgcggt tcattcttcc ggaatgtcat
     4621 ccagtctccc cgaattactc atttcatgca gcgatgacac gatgtcactg acaggcgtaa
     4681 caacgccccg acgctggctg gtcagaatat cggtttcagc tctgagttta tctctggcgg
     4741 cgttgattct ttcccggtca gcacgaagtt ttggtgctgt ctcagccagg acgtccagcg
     4801 tcagcaatga gcgttcaatt gactcgatac gggcaatatt ccggtcaagg gcctgttcag
     4861 ctttgagtat tttgtcgtaa agagcaac
//
LOCUS       DAZFVO020000252         4842 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZFVO020000252 DAZFVO020000000
VERSION     DAZFVO020000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.254"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            85..621
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS24055"
                     /old_locus_tag="pgaptmp_004811"
     CDS             85..621
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS24055"
                     /old_locus_tag="pgaptmp_004811"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4198179.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            612..974
                     /locus_tag="ACHKNB_RS24060"
                     /old_locus_tag="pgaptmp_004812"
     CDS             612..974
                     /locus_tag="ACHKNB_RS24060"
                     /old_locus_tag="pgaptmp_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198180.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            974..1279
                     /gene="yfdT"
                     /locus_tag="ACHKNB_RS24065"
                     /old_locus_tag="pgaptmp_004813"
     CDS             974..1279
                     /gene="yfdT"
                     /locus_tag="ACHKNB_RS24065"
                     /old_locus_tag="pgaptmp_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HGU4198181.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1195..1374
                     /locus_tag="ACHKNB_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /pseudo
     CDS             1195..1374
                     /locus_tag="ACHKNB_RS24070"
                     /old_locus_tag="pgaptmp_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1506..2669
                     /gene="intD"
                     /locus_tag="ACHKNB_RS24075"
                     /old_locus_tag="pgaptmp_004815"
     CDS             1506..2669
                     /gene="intD"
                     /locus_tag="ACHKNB_RS24075"
                     /old_locus_tag="pgaptmp_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGU4198182.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(2685..2761)
                     /locus_tag="ACHKNB_RS24080"
                     /old_locus_tag="pgaptmp_004816"
     tRNA            complement(2685..2761)
                     /locus_tag="ACHKNB_RS24080"
                     /old_locus_tag="pgaptmp_004816"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2725..2727),aa:Arg,seq:tct)
     gene            3004..3636
                     /gene="fimZ"
                     /locus_tag="ACHKNB_RS24085"
                     /old_locus_tag="pgaptmp_004817"
     CDS             3004..3636
                     /gene="fimZ"
                     /locus_tag="ACHKNB_RS24085"
                     /old_locus_tag="pgaptmp_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HGU4198183.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(3639..4154)
                     /gene="sfmF"
                     /locus_tag="ACHKNB_RS24090"
                     /old_locus_tag="pgaptmp_004818"
     CDS             complement(3639..4154)
                     /gene="sfmF"
                     /locus_tag="ACHKNB_RS24090"
                     /old_locus_tag="pgaptmp_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HGU4198184.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4165..>4842)
                     /gene="fimH"
                     /locus_tag="ACHKNB_RS24095"
                     /old_locus_tag="pgaptmp_004819"
     CDS             complement(4165..>4842)
                     /gene="fimH"
                     /locus_tag="ACHKNB_RS24095"
                     /old_locus_tag="pgaptmp_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HGU4198185.1"
                     /translation="VDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIR
                     PFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFG
                     DIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQ
                     DLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALG
                     YLRVDYQ"
BASE COUNT         1440 a         1097 c         1135 g         1170 t
ORIGIN      
        1 taattaactc aataatcacc ggatggtgag ggcttccttt taccagaatt cagcgcggtg
       61 cagtgcatat acgtggagaa caaaatgtca tttattaaaa ctttttccgg gaagcatttt
      121 tattatgaca agataaataa agacgacatc gatattaacg atatcgcggt ttccctttca
      181 aatatctgtc gctttgccgg tcatctttcg cacttctaca gcgtcgccca acatgcggtt
      241 ctttgcagcc agctggtgcc gcaggaattt gcttttgaag cgttaatgca tgatgcaaca
      301 gaagcgtatt gccaggacat tcccgcacca ctgaaacgcc ttcttcctga ctataaacgg
      361 atggaagaaa aaatagacgc cgtaatccgt gagaaatacg ggttaccccc agttatgagt
      421 acacccgtga aatatgccga tctcatcatg ctggcaaccg aacgccgcga tctcgggctt
      481 gatgatggct ctttctggcc tgtactggaa ggcatcccgg caacagagat gttcaacgtg
      541 attccactgg caccgggtca tgcctacggg atgtttatgg aacgttttaa cgatttatcg
      601 gagttacgca aatgcgcatg aatgttttcg aaatggaagg gtttcttcgc gggaaatgtg
      661 taccgcgaga tctgaaagtg aacgaaacaa atgctgagta cctgttacgt aaattcgacg
      721 cgcttgaagc taaatgtgcg gcactggaaa acaaaataat accagtgtca gctgaactgc
      781 caccagcaaa tgaaagtgtt ctgttatttg atgctaatgg agaaggctgg ctgattggct
      841 ggcgttctct ctggtacacc tggggacaaa aagaaaccgg agaatggcag tggacatttc
      901 aggtcgggga ccttgaaaac gtcaatatca ctcactgggc agtaatgcca aaagcaccgg
      961 aggctggagc ataatgacca catttaccaa taaagaactg attaaagaaa tcaaagaacg
     1021 aatcagcagc ctagaggttc gagacgatat tgagcgccgt gcttatgaaa tcgcactcgt
     1081 atctctggaa gtagagccag atgaacgcga agcctatgaa ttattcatgg aaaagcgttt
     1141 cggtgactta gtagatcgtc ggagagcaaa aaacggcgat aacgaataca tggcatggga
     1201 tatgactctc ggttggatcg tctggcagca acgagctggt atccattttt caacaatgtc
     1261 acagcaagag gtgaaataat ggagccatac agcctcacac tcgatgaggc ctgtcatttt
     1321 ctcaaaatat ccagaccgac tgccattaac tggatacgca caggccgcct acaggcaaca
     1381 cgtaaagatc caaccaagcc aaaatctcct tacctcacaa cacggcaagc ttgcattgcg
     1441 gcgcttcagt ctccgctgca tactgtccag gtgagcgcgg gtgatggcat aacagaggaa
     1501 agaaaatgtc actcttccgc agaaatgaaa tatggtatgc ctcgtattcg ctcccgggcg
     1561 ggaaacgaat taaggaatct cttggcacaa aggacaagcg gcaagctcag gagttgcacg
     1621 acaagcgaaa agcagaactc tggcgagtag aaaagctagg ggatttacct gatgtcactt
     1681 ttgaagaggc ctgcctaaga tggcttgagg aaaaagctga taaaaaatct ctcgattcag
     1741 ataaaagccg gattgagttc tggcttgaac attttgaggg tataaggctt aaagatatct
     1801 cggaggcaaa gatttactct gctgtaagca gaatgcataa cagaaagacg aaagaaatat
     1861 ggaaacagaa agttcaggcc gccatcagga aaggtaaaga accgcctgtt tatgaaccaa
     1921 agccagtatc aactcagaca aaggcaaagc atcttgccat gataaaggcc attctccgtg
     1981 ctgcagaacg cgactggaag tggctggaaa aagcgcctgt catcaagata ccagcggtca
     2041 gaaacaagcg agtcagatgg ctggaaaagg aggaagcaaa acgccttatt gatgagtgcc
     2101 ccgaaccact gaaatctgtc gtcaagtttg cgctggcaac tggtctgaga aagtcgaaca
     2161 tcataaatct ggaatggcaa caaatcgaca tgcagcgacg agttgcctgg gtgaatccag
     2221 aagagagcaa atcaaaccgc gccattggtg tggcgctgaa cgataccgcc tgtaaagtgt
     2281 tgcgtgatca aataggcaag catcacaaat gggtgtttgt acataccaag gcggctaagc
     2341 gagcagatgg aacatcaacg cctgcggtca ggaagatgcg catcgacagc aagacatcat
     2401 ggctatcagc ttgtcgtcgt gcaggaattg aagatttccg tttccatgac ctcagacaca
     2461 cctgggcaag ctggctgatc cagtcaggcg tcccattatc tgtgcttcag gaaatgggcg
     2521 gatgggagtc catagaaatg gttcgtaggt atgctcacct tgcgcctaat catttgacag
     2581 agcatgcaag gaaaatagac gacatttttg gtgataatgt cccaaatatg tcccactgtg
     2641 gaattatgga ggatataaag aaggcgtaac tgattgaatt gtaatggcgc gccctgcagg
     2701 attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga gctaagggcg
     2761 cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt gctgagtcaa
     2821 tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct gatttatttc
     2881 gctatagcgg caaacaaaca cacaccgctg cgcgtctgaa tcaagaaaac ccgtattttc
     2941 atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat gagactggta
     3001 actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag aatgtctatt
     3061 gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga tgattaccgc
     3121 ataaccatcg attatctccg aacccgtcct gttgatttaa tcattatgga tatagacttg
     3181 cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag cacagtgaaa
     3241 gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat acaagctggt
     3301 gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc cgttcagatg
     3361 atcctctccg gatacacgtt ttttcccagc gaaacgctta actatataaa aagcaataaa
     3421 tgtagtacga atagttcaac ggtcactgtg ctatctaatc gtgaagtgac catattacgt
     3481 tatctggtta gcggattatc taataaagaa attgccgata agttattact tagcaataaa
     3541 acagttagtg cgcataaatc taatatttat ggcaagctag gtttgcattc aattgtagag
     3601 cttatcgact acgccaaatt atacgaatta atataatatt aattataatt gatcataaat
     3661 atcgcatccg ctttcgccac acctggccga acaccgctgg ctaacgctcg ataattggca
     3721 aaaaagttta gtgtgacatt gccatttgca tctacttcct cagtcgggct cgccgctccc
     3781 agtgcgagcc gggagcgatc gctattacgt aattcgatgg cgacggtttg cgccattgcg
     3841 ggatcatcca gagccagcag gttggtatcg gatgccggcg ttcccgtaaa taaaatcgca
     3901 actgaacccg gaggacatcc ctccagccgc aggctaaaag ggacgagtgc cgtggtatcg
     3961 ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat ctaccgtctt atcaatatcc
     4021 gcagtgttta cggtacagga gaaatcaaca acgttaccgt gcaaattgat attaatcgtt
     4081 cccaaagggt cagctgccca tccactggaa ctccacagta acccgcagaa acagctaaag
     4141 agtactcttc tcattctccg tacctcattg ataatcgacg cgtaaatacc ccagcgcgct
     4201 aaacggccct tcggtcggtt tttgaccggt aatactgata ggccaggcgc gaagtgtgac
     4261 attggctgcc gcagctgcat ccagacggaa aggaataacg ctattgagat cgttaggcgt
     4321 gatcggcgta tcgttctgat cggcgacaat aaaacctaaa tcctgattgt ccgacaccat
     4381 cgcctgacca gaaacggcac tggcttccag acgcattgtt aaataagcct gcgcagcaac
     4441 attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg acaccagcag gtcgattacc
     4501 cggccctgcc gcactaaata acgatgcgcc gatatcacca aaatcaaatt caacaatctg
     4561 cccggcattt aattcgcagt tttgcggtac ttcaacccgg ccaccaaaac taatggtata
     4621 aacaggtgta accaacggat cgtaaggcgt tgatgtcaca tacacggtaa acatcacctg
     4681 tctggggatc tccaccatgt taataaaggg acgaataacc tttaaacgaa atattaaacg
     4741 agaatccatc accccgaatg gaaggttttg tgaaacgtta ggatctgttc ccatccgaat
     4801 ataattttgc ggggggaact gaatatccat cacactatca ac
//
LOCUS       DAZFVO020000253         4757 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFVO020000253 DAZFVO020000000
VERSION     DAZFVO020000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.255"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..231
                     /locus_tag="ACHKNB_RS24100"
                     /old_locus_tag="pgaptmp_004820"
     CDS             <1..231
                     /locus_tag="ACHKNB_RS24100"
                     /old_locus_tag="pgaptmp_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4198186.1"
                     /translation="SLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDAILDEL
                     LGAPAAPEPEGIAGEGSASDSGEPTPDSDMMM"
     gene            308..523
                     /locus_tag="ACHKNB_RS24105"
                     /old_locus_tag="pgaptmp_004821"
     CDS             308..523
                     /locus_tag="ACHKNB_RS24105"
                     /old_locus_tag="pgaptmp_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4198187.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGD"
     gene            527..1084
                     /locus_tag="ACHKNB_RS24110"
                     /old_locus_tag="pgaptmp_004822"
     CDS             527..1084
                     /locus_tag="ACHKNB_RS24110"
                     /old_locus_tag="pgaptmp_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198188.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSVLMPSEDEKKRRDEQFAAF
                     YDYCRKVMSRNLAECFSIHNDNFSELEWECNRPSFVVSGDGGKITISENGKVTPPSHQ
                     HSETLIEFAIDYLKNNKKQGLMKRIGRCMGYLQLAAEIEALASGADRDAAVREGLLCD
                     FNTPPFKKEPDGWIQPGLTYLKWRI"
     gene            1112..1339
                     /locus_tag="ACHKNB_RS24115"
                     /old_locus_tag="pgaptmp_004823"
     CDS             1112..1339
                     /locus_tag="ACHKNB_RS24115"
                     /old_locus_tag="pgaptmp_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016050279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HGU4198189.1"
                     /translation="MKKYDMTVKSFHMGDKNVVVVLGIYNYQVPSMLLFNIEIDIERR
                     EDATIPFYEALALKKAASLIHEISDDLAVAA"
     gene            complement(1344..1631)
                     /locus_tag="ACHKNB_RS24120"
                     /old_locus_tag="pgaptmp_004824"
     CDS             complement(1344..1631)
                     /locus_tag="ACHKNB_RS24120"
                     /old_locus_tag="pgaptmp_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198190.1"
                     /translation="MEISNHELFQLIQGLQRENIAINTAANFLFYNIVDILDQQSGDK
                     KFSDELKTRLHSELSKITTSGKPEIKVAINTLMQPPVRPMFGNTSPEPFLK"
     gene            1756..2289
                     /locus_tag="ACHKNB_RS24125"
                     /old_locus_tag="pgaptmp_004825"
     CDS             1756..2289
                     /locus_tag="ACHKNB_RS24125"
                     /old_locus_tag="pgaptmp_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4198191.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMTLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2446..2630
                     /locus_tag="ACHKNB_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /pseudo
     CDS             2446..2630
                     /locus_tag="ACHKNB_RS24130"
                     /old_locus_tag="pgaptmp_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2645..2776
                     /locus_tag="ACHKNB_RS24135"
                     /old_locus_tag="pgaptmp_004827"
     CDS             2645..2776
                     /locus_tag="ACHKNB_RS24135"
                     /old_locus_tag="pgaptmp_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198192.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAICFSTAWRVLTLSGLVG"
     gene            2823..3719
                     /locus_tag="ACHKNB_RS24140"
                     /old_locus_tag="pgaptmp_004828"
     CDS             2823..3719
                     /locus_tag="ACHKNB_RS24140"
                     /old_locus_tag="pgaptmp_004828"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGU4198193.1"
                     /translation="MPSLLPGSVDLVLCDPPYGTIKGLTLRTWDKSTTAWDDALNPED
                     IFTQCERLLRVGGVLVMFSQEPHTSTMITLADKNLPFLYRMVWKKEHFPNPLSVKKAP
                     VSLFEDIVVFRKKYDSEKNHPLREYARNILSFIGHRTDKEVNKTLGHRRAEHFFCVNT
                     LQFSLCSQDTYNELIKRFGIDRMPGFMTYEEMRKVNSGYGTVVFNLPQGKKYRANILE
                     YARERERWHPTQKPVALLEELIQTYSNPGDRVLDFTMGSGSTGVACIRTGRRFTGIEK
                     DDRYFAVAKKRLEKENSEMVVI"
     gene            3936..4121
                     /locus_tag="ACHKNB_RS24145"
                     /old_locus_tag="pgaptmp_004829"
     CDS             3936..4121
                     /locus_tag="ACHKNB_RS24145"
                     /old_locus_tag="pgaptmp_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4198194.1"
                     /translation="MRELKMKLCVLMLPLVVSACGLKPPVQAPCVKPPPPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            4207..4431
                     /locus_tag="ACHKNB_RS24150"
                     /old_locus_tag="pgaptmp_004830"
     CDS             4207..4431
                     /locus_tag="ACHKNB_RS24150"
                     /old_locus_tag="pgaptmp_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198195.1"
                     /translation="MKKLLVTVKSFQGTIPFRILQCGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT         1246 a         1003 c         1307 g         1201 t
ORIGIN      
        1 tccctggtca ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt cctgagcgca
       61 ctgaaacaga aacttcgcca taacctggaa gcgcgtcttg atgcagaagt ggatgccatt
      121 ctggatgaac tgctgggcgc accggcagct ccggagccgg aaggcatcgc gggtgagggg
      181 agtgcttcag atagcggtga acccacacct gacagcgaca tgatgatgta agcatgcgca
      241 agggaccatc ggtgtgtgcc ggtggtcttt atattgttgt gagcttccgg attgcgggag
      301 acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt
      361 gggaacgggc tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc
      421 aataggcgtg ctggggagtc tgctgtttgg gctgctgaca tatctgacga acctgtattt
      481 caaaatcaga gaggaccggc gtaaggcggc acggggagat taggtgatga accatgaaga
      541 aatgaatcag cgcttcagtc gtctggaaaa tgaaattgct gaactgaata aaaaactgtc
      601 ggtgctgatg ccttctgaag atgaaaaaaa acgccgcgat gagcagtttg ctgcgtttta
      661 cgattattgc cggaaagtga tgagcagaaa tctcgcagag tgtttcagta ttcataatga
      721 taatttcagt gagctggaat gggagtgtaa ccggccatcc tttgttgtat ccggtgatgg
      781 tgggaaaata accatctcag aaaatgggaa agtaacacct ccatcgcacc agcacagtga
      841 gacgctcatt gaatttgcca ttgattacct gaagaacaat aaaaaacagg ggctgatgaa
      901 gcgcattggt cgctgcatgg gatatctgca gctggcggca gagattgaag cacttgccag
      961 tggtgcagac agggatgctg cggtgcggga gggtcttctt tgtgatttca atacgccacc
     1021 ctttaagaaa gagcctgatg gctggatcca gccggggctg acttatctca aatggcgcat
     1081 atagtaaaag agaaaacaat tgaggtgaaa aatgaaaaaa tacgatatga cagttaaatc
     1141 attccacatg ggggataaaa atgttgtcgt tgtccttggt atatataatt atcaggttcc
     1201 atcaatgctg ttgttcaata ttgaaataga tattgaacga cgggaagatg ctaccattcc
     1261 gttttatgag gcgctggctt taaaaaaagc agcctcgcta attcatgaaa tttctgatga
     1321 tttagcagtg gctgcttaga tttttatttc aaaaatggct caggactggt attgccaaac
     1381 atgggtctta ccggtggttg catgagggta ttgatggcga ctttaatctc tggtttacct
     1441 gaggttgtga ttttgctcag ttcgctatgt aacctggttt taagctcatc actgaatttc
     1501 ttgtctccac tttgctgatc cagaatgtca acgatgttgt agaacaggaa attggctgcg
     1561 gtatttatag ctatgttttc gcgctgaagc ccctgaatta gttgaaaaag ttcatggttt
     1621 gatatttcca ttaagttctc cttattgagg ttatcagcca tccctctttt gttacatgtg
     1681 ccagtgccca ccactggcag gctgaagact taacatatcc agggattcgg aaccgataaa
     1741 tcctgataaa tatccatgaa caccaaaatc aaatacggcc tgtcggctgc cgttctggcg
     1801 ctgattgccg caggtgcgcc tgcgcctgaa atcctcgacc agtttcttga cgaaaaagaa
     1861 ggtaaccaca ccacggcata ccgtgatggt gcgggtatct ggaccatctg ccgaggtgcc
     1921 acccgggtgg atggtaagcc tgtgattcct ggcatgacgc tgtcgaaggg gaaatgcgac
     1981 caggttaacg ccattgagcg tgataaagcg ctggcatggg tggagaaaaa catcagagtg
     2041 ccactgaccg aaccccagaa agcggggatc gcgtcattct gtccttacaa cattggcccc
     2101 ggtaagtgtt tcccgtcgac gttttataaa cgaattaatg caggcgatcg caggggagcg
     2161 tgtgaagcga ttcgctggtg gattaaggac ggtggcagag actgccgtat ccgctcaaat
     2221 aattgttatg gtcaggtatc ccgtcgtgac caggagagcg cgctggcgtg ctggggtatc
     2281 gacagataag cagaatattt tgctgaaaaa tgacgttgac taacgcggac ggataacacg
     2341 aaatcctgcg aactggcaaa acctaagtga ataaaagtaa aaaccccgtt tgttggcagc
     2401 aagcggggtt ttgtgtttct gaccttggat aaggcaaggg agaacatgga aaagtataaa
     2461 cgaattctgt tgaggttgac tatgaaaaac ggccttgaac tgaaagcgcc agagtaactg
     2521 atgacatcag cagagcgctg gcttttgcta ttaagtgggt ggcggttggt atcgctgtgt
     2581 ctccgatact gtatgggctg gcaaaactgg tcattgcgtt gaaatcgtga agggaggatt
     2641 aagcatgtca gacaaactca taatgctggc gaagatcctc tgtgtaatcg tcggcatttc
     2701 attttcacta atgctggttg ctatttgctt ttccactgcc tggcgcgtct taactctgtc
     2761 agggctggtg gggtgacaga aacatgaaat gtgtactgta ttccggtgac tgtcttgatg
     2821 ttatgccttc attactaccg ggaagtgttg acctggtttt atgtgacccg ccgtatggaa
     2881 ccattaaagg gcttacactc cgtacatggg ataagagtac gacagcatgg gacgatgcct
     2941 taaatcctga agatattttc acacagtgcg aacgactgct gagggtgggc ggtgtgctgg
     3001 tcatgttcag ccaggagccg catacaagca cgatgattac tctggcggat aaaaacctgc
     3061 cgtttttata tcgtatggtg tggaagaagg agcatttccc caacccgctt tcagtaaaaa
     3121 aagcacctgt atctttattc gaggatatcg ttgttttccg gaaaaaatat gacagtgaga
     3181 aaaaccaccc gctgagggag tacgcccgaa atatcctttc tttcattggc cacaggacag
     3241 acaaggaagt taacaaaaca ctggggcacc gtcgggcaga gcattttttt tgcgtgaaca
     3301 cactccagtt ttccctctgc tcacaggata cgtataacga gttaattaaa cgctttggta
     3361 tcgaccgtat gccgggtttc atgacatatg aagaaatgcg caaagtgaat tccggttacg
     3421 gcactgtggt ttttaatctc cctcagggga aaaaataccg cgcaaatatt ctggagtacg
     3481 cgcgggagcg ggagcgatgg catccgaccc agaagccagt tgcattactg gaagagctga
     3541 ttcagacgta cagcaaccct ggcgacaggg tactggattt tactatgggg agtggttcaa
     3601 caggtgttgc atgtatccgg acggggagac ggtttaccgg aatagaaaag gatgaccggt
     3661 atttcgctgt tgcaaaaaaa cgtctggaaa aagaaaacag tgaaatggtg gtgatatgaa
     3721 ccgtgttctg tgtgtggtga ttattgtcct gctggtggcc tgtggtgcgc ttagtctggg
     3781 gctgaatcat taccgcgata acgccatcac ctacaaagcg cagcgcgata aaaaagccag
     3841 agagctggag ctggcaaacg caaccattac tgatatgcag gtgcgccagc gcgatgttgc
     3901 tgcgctcgat gcaaaatact cgagggaatt agccgatgcg agagctgaaa atgaaactct
     3961 gcgtgctgat gttgccgctg gtcgtaagcg cctgcgggtt aaagccacct gtccaggctc
     4021 cgtgcgtgaa gcccccacca cctccggcgt ggataatgca accggccccc gactggcaga
     4081 caccgctgaa cgggattatt tcatcctcag agaacggttg atgacaatgc agaagcagct
     4141 ggaaggggcg caggaatata tccgcactca gtgtattaac tagtattttt gttatccgga
     4201 gaatgcatga agaaattact ggtaaccgta aagtcttttc agggaacaat tccgttccgt
     4261 attttgcagt gtggtcgtgt tcttgttgaa ggttcgttca gtggtaaatg tacgcaatta
     4321 cactcccgga cctttcaggt gaatgccacg aatgaagagc taaccgttga gtgtacgatg
     4381 aatgccgcta aatgccgcat ggtatccgct gcattacagc cagtgtgttg agcgacctta
     4441 ttatccatgc gcggtattgt cgccgtattc ctgcattaac agagaccgca gcccgaccgg
     4501 gagactcctc tgcgcgagtg tgcggggata atcaaaaacg atacacaccg gggtttaccg
     4561 cgttaacgga gcgcggcgtt gtcccctcat ggtcgctggt ccggtgcgat ggtggaagaa
     4621 accggacgat gtgttacctc gcaagctctg ttatgtcatg tgtctgattt gtgatttaag
     4681 tcggataatt gtcgttgcca ttaagcagag gattgatgac cgacagggtg gcattgttag
     4741 aataagactt attctta
//
LOCUS       DAZFVO020000254         4754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFVO020000254 DAZFVO020000000
VERSION     DAZFVO020000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.256"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1319
                     /locus_tag="ACHKNB_RS24155"
                     /old_locus_tag="pgaptmp_004831"
     CDS             <1..1319
                     /locus_tag="ACHKNB_RS24155"
                     /old_locus_tag="pgaptmp_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HGU4198196.1"
                     /translation="DNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVA
                     YNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYNDFYDIGRKNSLSAN
                     IMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKE
                     EERFNLFISISFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYG
                     IYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGINIT
                     NQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESET
                     ELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVV
                     GQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1332..2291
                     /locus_tag="ACHKNB_RS24160"
                     /old_locus_tag="pgaptmp_004832"
     CDS             1332..2291
                     /locus_tag="ACHKNB_RS24160"
                     /old_locus_tag="pgaptmp_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HGU4198197.1"
                     /translation="MKWLLLITLSLYSFIVQSTPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFEYDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2329..3228)
                     /gene="nhaR"
                     /locus_tag="ACHKNB_RS24165"
                     /old_locus_tag="pgaptmp_004833"
     CDS             complement(2329..3228)
                     /gene="nhaR"
                     /locus_tag="ACHKNB_RS24165"
                     /old_locus_tag="pgaptmp_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HGU4198198.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3294..4460)
                     /gene="nhaA"
                     /locus_tag="ACHKNB_RS24170"
                     /old_locus_tag="pgaptmp_004834"
     CDS             complement(3294..4460)
                     /gene="nhaA"
                     /locus_tag="ACHKNB_RS24170"
                     /old_locus_tag="pgaptmp_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HGU4198199.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGNVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1442 a         1073 c          991 g         1248 t
ORIGIN      
        1 ccgataacta taatgctata actctgggaa atggttggaa tacaccgctg ggtgccatct
       61 ctttttacgc tgcccgatcg agtagtaagt taaataatga taccagacac gaaggtacga
      121 gctatcaggt cgcctataat aaatatctag tacaaacagc cactcgattt agcgtggccg
      181 cctggcgtta tgcttcgcag gattacagga cattcagcga ccatctttac gaaaatgata
      241 aacactatca tcagagtgat tataatgatt tttatgatat tggcagaaaa aatagccttt
      301 ctgccaatat tatgcaacct ttatccaata atctgggaaa tgtatcatta agtgcgcttt
      361 ggcggaatta ctgggggcga agtggaaatg ctaaagatta ccaattcagt tactccaata
      421 actggcaaca cattagttat actttctctg ccagccaatc ttatgatgaa aataataaag
      481 aagaggagcg ttttaatctg tttatctcca tttctttcta ctggggggat gatattgcca
      541 aaacacgtca ccaaattaac ttatcgaatt cgacctcatt ttccaaagat ggctattcct
      601 ccaacaatac tggaattact ggcatagccg gtgaacatga tcagttaaat tatggtatat
      661 atgttaatca gcaacaacaa aataatgata cctcgcttgg tacgaattta agctggagaa
      721 ctcccatcgc cataatagat ggcagctata gtcattctaa aaacgcctgg caaagtggtg
      781 gaagtattag tagtggatta gttgtctggt ccggtggtat taatatcact aaccagttat
      841 ccgatacatt tgcaattctg gatgcgcctg gattagaagg tgcacatatt aatggacaaa
      901 aatacaatcg aacaaacagc aaaggtcagg ttgtttacga cctgataata cctcatcgtg
      961 aaaaccatct ggtacttgat atagcaaaca gtgaaagtga aacagaattg cagggcaatc
     1021 gtcaaattat tgcaccttac cgtggagcag tttcttatgt gcagtttaca actgaccaac
     1081 gtaagccctg gtatatacag gcactgcgtc ccgatggttc gccattaacc tttggctatg
     1141 acgtactgga tctccaggaa aataatattg gagtcgttgg ccagggtagt cgccttttta
     1201 ttcgcgtaga tgaaattccc actggaataa aagttgctct caatgatgaa cagaacttat
     1261 tctgtactat tacttttcaa cacgttatcg atgaaaacaa aacatatata tgccagtaaa
     1321 aggagtttac tatgaagtgg cttttattaa tcaccttgag tctatattct tttattgtgc
     1381 aaagtactcc ctgtgcattg accaatgtcg gtgaacaacg tggaacatat atactcaagc
     1441 cactgagtat gaaaggtaat ctaaccgccg aaaaaattga aaacaccgga aatatcattg
     1501 agtgtacaga cttgcaagag acaaccaacg ccattaaagt tcatttttta acacagaacg
     1561 ctttaaggtt taaagtcgga agtcgtcatt atattattaa tttccccttt gaatatgacg
     1621 gtaaaaaaat agagcttcca gggaagtcat cttataattt agaagaaatg ctatctgcga
     1681 taaatttcac cattccatat tctatcgcat ctgtatcaga tagttctaac accgcagaca
     1741 tttttccagg aactcctttt cctttgttgt tcgaaattga agtgagttca tgtacaggga
     1801 ataatactaa ttgtaatagc gtccgcttta attataattt aaatactatt ttacaggtaa
     1861 atttaatgac ctgtggtttt gaagatcaat ccatcgacac tggacgattt gtactgtctc
     1921 gtatcaataa tgaaaattat cagtttcatc atataagatt tgactgcatc cagggcgagc
     1981 aaggtgaatt agtttttgaa cccagtgatg ttgaatatta ctttgaacca gtgacgatac
     2041 aggagtcagg aaccagcata ttaaaaaatg agttagaaaa cagtgaggac ggtgcgggtc
     2101 aagtaggctt tcaactctcc aatgatggta cgaatgaaat ccagtatggt aaaagcaatt
     2161 actactcttt tcagcatccc catgaaggaa gtaatcagat tcctctattt attcgcccgc
     2221 gtacatatgg aaataatgtc tcaagcgggc aaataatgag ccgagtcaaa atcgtggtta
     2281 tgtacaacta atctatctcc tccactttaa ctttggggga gctgccgatt aacgcgccgc
     2341 tggactaaaa agcgcagaat aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat
     2401 catccgctca gcaaaaatag catggtactc ttccatcaca ttctcgacgc gaccaatttc
     2461 tacgacagtt ttatcggcat aaaagtcata tgcataaagc gttggggcaa cgaagattgc
     2521 attgtgcatt gcaccaaaag ctttcatcaa cgcggcatca tcaaactcgc cgaggatttc
     2581 tacgtttaat ccctgggagt taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg
     2641 cccaggaatc aaaagtcgcc gttcttccag acaagccggg aacggttttt ctggtggtgg
     2701 atttgtacac cagaaactca cgccacattc gccaattctc acggagaaca ggccttcctg
     2761 ctgcgtagag tctatcggac agtcggaaat gatcatatcc agtttatgct ggcttaattg
     2821 ctccagtagc atttcgtggg tggattcgaa gcagcgaaga tgaatcggct cgccttctac
     2881 cactgcagca ttcagcacgc tactgaccag gcgtttggaa agtgcatcag ccacgccaac
     2941 gtcaaacaat aaattggatt ctttgcgata gttcacaata tccagcattt cctggcttaa
     3001 ggtgaacatt ttatcggcat agcgatagac cagttctccc agctcgctgg gttcgagacc
     3061 acgtcccttg cgtttaaata atttgccttg caggcgctct tccagcgctc gaatctgtcc
     3121 ggtaatggtt tgtggagtta aataaagcgc ctccgctgcg ccaaccacgg aaccttcttt
     3181 atagacatgc cagaagtaat acaagtggtt gtaattgata tgagacatgc tcatttctct
     3241 ccctgataac aatgaaaagg gagccgttta tggctccccg gtaaaccgtc ctgtcaaact
     3301 gatggacgca aacgaacgcg taaccagctg tatccaatta ccgccgaaga gatagaaccg
     3361 accaggatac cgagtttcgc ccagttaatc agttctggat ctacgttacc aaaggccagg
     3421 ctggcaataa agatagacat agtaaaaccg ataccgcaca ggatccccac cgccataatt
     3481 tgctgataag tcgttccctc aggcagatgc gccagtttca aacgcagcgc caaccagcag
     3541 aacagactaa tccccagcgg tttgccaatc agcaagccag cgatgatccc caatggcaga
     3601 atggaggtca agccatccag cgtgacacct tgcagtgaaa cgccagcatt agcaaatgca
     3661 aacagcggca aaatcagata cgccacccac gggtgtaaca catgctccag tcgcttcgct
     3721 ggagaacgcc catgcttctc tttcaaagga ataaagaagc cgacaattac ccccgccagg
     3781 gttgcgtgaa cccccgattt caacaccgca gtccacaaca ctacgccaac cagaatatag
     3841 acgcccgtgc ggcgtacacc acacagattc aataccgcga gtaccgcaat tgctacagcc
     3901 gcgacgccaa gagaggccat cgataagtca ttagtgtaga acaatgcgat gataatgatg
     3961 gccccaagat cgtcgataat agccagagcc atcaaaaaga tcttcagcgc taacggaaca
     4021 cgacttccca acagcgccag cacaccaagt gcaaaggcaa tgtcagtagc cgccgggatc
     4081 gcccaccctt cgcgggtaat cggatcggca tagttaaagg ccagatagag taatgccgga
     4141 acaatcatcc caccaatagc ggcgataact ggaaatgcgg cctggcgtaa gctggctagc
     4201 gatccttgca tcagttcacg tttaacttcc agaccgacta acaggaaaaa taccgccatc
     4261 agcgcgtcat ttatccataa cagcatgttt ttgttgattt cgagtgaacc aacccggagc
     4321 tgaaccggcg tctccagaaa gtcgtgatac catccactgg ttgcgccgct attggccata
     4381 atcatcgcca ggatagcggc aatgataaga ataatgcctc ccgaggcatc actgctaaag
     4441 aatcgatgca gatgtttcac tttttatttc tctttcaggt gaatagatcg tttttactat
     4501 tttaccccgc acaaatcatc gttaaaatca gattatagtg taggaataac tcgtttttta
     4561 cgatccattg aagaatttgc gccatcagcg gtgagatgag tttttgacgg ctgtaagcgc
     4621 tcacgagtga ccctaagccc agataatcat agcttaaggt gaggatttct gagagattaa
     4681 ttaggaaaag taataattaa tttgttgtca gtcggcgatg tttgttaggc caatatttca
     4741 aaaataatcc agct
//
LOCUS       DAZFVO020000255         4685 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFVO020000255 DAZFVO020000000
VERSION     DAZFVO020000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.257"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(124..336)
                     /locus_tag="ACHKNB_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /pseudo
     CDS             complement(124..336)
                     /locus_tag="ACHKNB_RS24175"
                     /old_locus_tag="pgaptmp_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            486..1154
                     /locus_tag="ACHKNB_RS24180"
                     /old_locus_tag="pgaptmp_004836"
     CDS             486..1154
                     /locus_tag="ACHKNB_RS24180"
                     /old_locus_tag="pgaptmp_004836"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGU4198200.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1099..1236)
                     /locus_tag="ACHKNB_RS24185"
                     /old_locus_tag="pgaptmp_004837"
     CDS             complement(1099..1236)
                     /locus_tag="ACHKNB_RS24185"
                     /old_locus_tag="pgaptmp_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HGU4198201.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1209..1793)
                     /locus_tag="ACHKNB_RS24190"
                     /old_locus_tag="pgaptmp_004838"
     CDS             complement(1209..1793)
                     /locus_tag="ACHKNB_RS24190"
                     /old_locus_tag="pgaptmp_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4198202.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTTWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEGE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1793..>4685)
                     /locus_tag="ACHKNB_RS24195"
                     /old_locus_tag="pgaptmp_004839"
     CDS             complement(1793..>4685)
                     /locus_tag="ACHKNB_RS24195"
                     /old_locus_tag="pgaptmp_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4198203.1"
                     /translation="SAGQAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSA
                     GAAKTSETNASASQQSAATSASTATTKASEAATSARDAAASKEAAKSSETSAASSASS
                     AASSATAAGNSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQAS
                     ASATAAGKSAESAALSASTATTKAGEAAEQASAAARSASAAKTSETNAKASETSAESS
                     KTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKS
                     TAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKSVKS
                     AYDNAEKRLQKDQNGADIPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKY
                     YPVVVMRSAGSVSELASRVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFW
                     QYQNNERAIHSIMMSNKGDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTY
                     KFGATIPATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIR
                     MIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNG
                     NALTATKLQTPRLVSGVEFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEGWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSGVIPDMRGWTIK
                     GKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSG
                     STNSAGAHTHSLANVNTASANSGAGSASTRLSVVHNQNYATSSAGAHTHSLSGTAASA
                     GAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         1090 a         1210 c         1101 g         1284 t
ORIGIN      
        1 catatttttg ttattcttgt atgaaatcgt ctcatgtaca cggtcctttt ttcgccgaaa
       61 actgatatta ctttaatcaa aagttgacgt gccagattcg aagtaaattt ataaaatatc
      121 aaatcaatat acattttgca ccttcgagat agtaatggct gttactgtaa catgagttac
      181 aaacagcttc atacccagag ctactgatgt atcaacacga ttcaacaata cccgatattt
      241 attccatgcc tccagcaacg atctttcttc ctccgttgcg atttccagat ctacagcatc
      301 ctgcagtggc gcaatatact cactgaattc ctggatgtag aactgtgtgg tgacggtctt
      361 ccagctattc ggctcctgct gtatcgaagc ataccaggct atttcaatat cgctatgctg
      421 cggcagcatt taaccccttg taattcatcg ccataattga tttaattcac aaataaaact
      481 ataacatggt gaaattaatg aaaaaaaaca cagatgatgg ggctaaaatt tacacaccac
      541 ttaccctaaa gctttatgac tggtgggttt tgggagtatc aaatcggctt gcatggggat
      601 gtcctacaaa ggaacacctt cttccacact ttctggaaca tttaggtaac aaccatctgg
      661 atattggtgt tggaactggg ttttacctta ctcacgtacc tgagagtagt ctgatatctt
      721 taatggattt gaacgaagct agcctgaacg cggcatctac aagggctggg gaatcaaaaa
      781 ttaaacataa aattagccat gatgtttttg aaccttatcc cgcggcgtta catggtcaat
      841 ttgattccat ttccatgttt taccttcttc actgcctgcc tggaaatata tctacaaaaa
      901 gctgtgtaat acgcaatgcg gcgcaggcct taactgacga tggaactcta tacggagcca
      961 caattcttgg cgatggagtt gtgcacaata gcttcggtca aaaactgatg cgcatttaca
     1021 atcagaaagg catcttttca aacacaaaag attccgaaga aggcttaaca catatactct
     1081 cagagcattt cgagaatgtt aaaaccaagg ttcaaggtac tgtagtaatg ttttccgctt
     1141 cagggaaaaa atagcatcca accgcagcac gttcttgctt aagacgtgct gcggcataat
     1201 cccaatgatt actccctgac agggttcgta ggccactcaa tatcaggtgc agttgatgta
     1261 tcaacacggt tcagcaacac ccgatacttt ttccaggctt ccagcaacga ggtttctccc
     1321 tccgttgcga tttccagatc tgcagcatcc tgaagtggcg caatatgctc actggctacc
     1381 tgcatcaggc tgttttttgt ttcttccgcc tcccggatcc ggaacagttt ttctgcttct
     1441 gcatctttca cccaggttgt gccgttccac ttctgaaact ccccttccgg ggataaccag
     1501 gtgacatttt ccggtaatga gccgagttca gaaataaata acgcgtcgcc ggaagccacg
     1561 tcatagacgg ttttaccccg atggtcttca acgagatgcc acgatgcctc atcactgttg
     1621 aaaacagcca caaagccagc cggaatatct ggcggtgcaa tatcggtact gtttgctggc
     1681 agacctgtat gaggcggaat atatgcgtca ccttcaccaa taaattcatt agttccggcc
     1741 agcagattat aaatttttat ggtccgtggt tgttcactca ttctgaatgc cattatgcaa
     1801 gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt
     1861 taacggtgat ggtgtgtcca tgtgagccaa tcgcaaccga gtgcgtatga gcaccaatac
     1921 cgacagtatg tgcgtgtgca cctgcgcttg cagcagtgcc ggacagtgag tgggtatgtg
     1981 cgccagcaga tgatgttgca tagttttgat tatgcacaac agacaatctt gttgatgcac
     2041 taccagcacc ggagttagca ctagccgtgt tcacgttggc tagtgagtgt gtgtgtgctc
     2101 cagccgagtt tgtagagccg ctcacactgt gtgtatgtgc cccggtgtta ttcgtagatt
     2161 tagtgccgta atcaaacgac gatgtggttt tcgtacccaa atccgtactg gatgcgctgg
     2221 cgctgtgggt gtgcgattta atgccgtcct gttcctgaga caatacggca cgaccactgg
     2281 cgggcttgcc cttaatcgtc cagccacgca tatcagggat cacgcctgac ggataagcaa
     2341 ctgcaagttt cgggtatgca gatttgtcaa aagtctgccc ctgcatcagg gcataaccag
     2401 acggaacggt atctgatggc cacggaattg gtgcgccaac tgggtagctt tctggtggaa
     2461 gatttttcga ggtataaact tctgcccagc cttcctcaaa accatagcca tctcttgagg
     2521 aacggtagaa cagacctcca tttctgtaat gcgccttcat ctgcagggtc cggcaacttc
     2581 cgactccggt atagaagtta accagaatat agctgtcgcc agagcgggtg acattgtaag
     2641 cgcctgattc ggcattccag ggaacgccac catccgcatc ggcatatgta tccgttgccc
     2701 ttctggcaaa agcagccaca tgcgcggcgg ttaaagtaat atcttttgaa ccatcaaact
     2761 caaccccaga aaccagtctt ggcgtttgca gctttgttgc tgtcaatgcg ttaccgttca
     2821 gacttgcgga cagtttggtt ccaataacca gttcgccggt tgcgttatca atagcaaacg
     2881 gtcttaatgt attccagcca ccataaacat caccttgatt ggtaagcagc aggtaagttt
     2941 tagcgccatc attacgccat aatgccccat actccccacc tatcattcga atctgattac
     3001 caccacgcgc tacaatttcg tctgtggcaa aaagtttttt gcacgacaag ttatcgttaa
     3061 cgattaacga atgggactca taaaaaccac gcccactctt aaaatcaagg ataacgtccg
     3121 ccgcgataca ttcagtcgct ggaattgttg ccccaaactt ataggtcgta tcattaacaa
     3181 cgagatcagc accaggtgcg gatattgaca ggccatcttc gataaacgca aaaacaggga
     3241 aagcagcgcc atcaacatag aacacagagc gcaaatcatc gcccttatta ctcatcatta
     3301 ttgagtggat ggctcgttca ttgttttgat attgccagaa cattccataa gcataacgcc
     3361 ccctgtcagt ccagccacca ggcataacaa atccgttaaa ctcgcagtta ttcatcggat
     3421 cgcctgcggt tcgcgttgcc gtggtgataa tgacccttga tgccagttcg cttactgagc
     3481 cagcagaacg cataacaaca acagggtaat attttccaga tgttgcacct gcaggagcgt
     3541 taacccgcac ataacgcata ccacgcttat cagcaaagtc tgttttactg accgcgttaa
     3601 tgttgttcag gaagcgtccc ttatcgggta tatcagcgcc gttctggtct ttctgcagac
     3661 gtttctctgc attgtcatag gctgatttta ctgactttgg cgttgccgcc agcgtttcag
     3721 acgtactgtt ggtcgcactg ctgagctgta ctatcccctt tttcgtcgta cttgcatcct
     3781 caagcgccac ggcggatgca atatcctctg cccgtttagc tgctgtctcg gcgcgcgttg
     3841 ccgcggattc cgccgtactt ttgctctgtg ctgccgctgt cgcactgcca gcagcctctg
     3901 tcgccttcgt ggatgccgtc gtggcgctgc ccttcgctgc tgacgcctgt ctggtcgcct
     3961 catcttttga agcagacgca gatgatgccg atgacgccgc cgaactggca gacgatgccg
     4021 ctgccgtttt tgaggattct gcgctggttt ccgacgcttt cgcgttcgtt tcggatgtct
     4081 tcgctgcgga tgcagacctc gctgctgcgc tggcctgttc agcggcttcg ccagccttcg
     4141 ttgtggctgt tgaagcggac aatgcagcgc tttctgccga ttttccggcg gcggtggcac
     4201 tggctgaggc ctgcccggca cttgttgacg ctgcactggc agacgacgca gccgctgttt
     4261 ttgagcctgc cgcagctgag gcgctctgtc ccgctgccgt ttcagaagac ctggcgttcg
     4321 tctcggacgt ttttgccgcc ttcgcggaat ttcctgccgc cgttgccgag gaagctgcac
     4381 tactggcgct cgaggctgcg ctcgtttctg atgattttgc cgcctctttt gaggccgccg
     4441 catcccgggc cgaggtcgca gcttctgatg ccttcgtggt cgcggtggat gcagatgtgg
     4501 ctgctgattg ttgtgacgct gaagcattcg tttctgacgt tttcgccgca ccggcactgg
     4561 tggccgccgc gctttttgag gactctgcag cggcagcact ttttgatgct tcagtggcct
     4621 ttgttgatgc cgttcctgcg ctggaagacg ctgactgagc cgacgacgcg gcctgtccgg
     4681 ctgac
//
LOCUS       DAZFVO020000256         4592 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFVO020000256 DAZFVO020000000
VERSION     DAZFVO020000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.258"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..441)
                     /locus_tag="ACHKNB_RS24200"
                     /old_locus_tag="pgaptmp_004840"
     CDS             complement(<1..441)
                     /locus_tag="ACHKNB_RS24200"
                     /old_locus_tag="pgaptmp_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HGU4198204.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVA"
     gene            670..1278
                     /locus_tag="ACHKNB_RS24205"
                     /old_locus_tag="pgaptmp_004841"
     CDS             670..1278
                     /locus_tag="ACHKNB_RS24205"
                     /old_locus_tag="pgaptmp_004841"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HGU4198205.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1376..2767
                     /gene="selA"
                     /locus_tag="ACHKNB_RS24210"
                     /old_locus_tag="pgaptmp_004842"
     CDS             1376..2767
                     /gene="selA"
                     /locus_tag="ACHKNB_RS24210"
                     /old_locus_tag="pgaptmp_004842"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HGU4198206.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2764..>4592
                     /gene="selB"
                     /locus_tag="ACHKNB_RS24215"
                     /old_locus_tag="pgaptmp_004843"
     CDS             2764..>4592
                     /gene="selB"
                     /locus_tag="ACHKNB_RS24215"
                     /old_locus_tag="pgaptmp_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HGU4198207.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDAL"
BASE COUNT         1008 a         1189 c         1367 g         1028 t
ORIGIN      
        1 cgccacgccg ccgcgcacca gccagagtga ttcgctgcgg ctgtaaccgt cctcaccggg
       61 aaacaggtgc tcaaaataca ggcttctgcc gccgttatca ctgagtatca gtgtgttatc
      121 gcgcagctgt aagcggatat ccgcaggcat tttccagccg gggccgaggc tccccaccgg
      181 cgcgggcgtt tttgtccggt aactgctgta ggtgcgggag aggatgaacg gcagcggggc
      241 gggcagggcg atgtcggttt caccgggaag gacctttgca ccgagcaggg gattgaccgg
      301 atggccggac gtcactccgc cggggcacac cgaacaggcc acgccggtgg gggcaccgat
      361 gcgcaccccg gctgaaccct ggacaatgct accgccatac tgcgtcatgt cgccctgacg
      421 cgctgccggt tttccgctca tctgctcatc cttgttttat cgggttattt gtctgcttcc
      481 aggcaaaacc ctacagattt ataagaaagc cttaagtgag atatccatca gatagaaaca
      541 attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag taacaaacca
      601 tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc ctcgcacaaa
      661 ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa actttctatt
      721 ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta taacgcggac
      781 aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac cgaagagggc
      841 gaatgttggt ttgattcgcc gatcatcgct gaatatattg aattaatgaa tgtcgctccg
      901 gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga ggcactggcg
      961 gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc agcggcgcag
     1021 cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct ggatgtgctg
     1081 gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc gactatcgcc
     1141 attgcctgtg ccgtcggata tctcaatttc cgccgcgttg cgccaggctg gtgtgtcgat
     1201 cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc
     1261 gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt tacaatccgt
     1321 ggttatgtta aacgcccttc tccgtgtgag agggccttga tcagccaggt ttcctatgac
     1381 aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat tgcgcgatag
     1441 ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat tgttgcgtca
     1501 gatgctcgac gaagcgcgag aagtgattcg tggcagccag acgctgcctg cgtggtgtga
     1561 aaactgggcg caagaagtcg atgcccggtt gacgaaagaa gcgcagagcg cgctgcgtcc
     1621 ggtgatcaac ctgacgggaa ccgtgctgca taccaacctt gggcgagctt tacaggcgga
     1681 agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg agtacgatct
     1741 ggacgacgct ggacgcggac atcgcgatcg tgcgctggcg cagttgctgt gccgcattac
     1801 gggggcggaa gatgcctgta tcgttaataa caatgcggcg gcggtgttat tgatgttggc
     1861 ggccactgcc agcgggaaag aggtggtggt ttctcgcggc gaactggtgg agattggcgg
     1921 cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg aagtagggac
     1981 caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata ccgcactgtt
     2041 gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga tagatgaagc
     2101 ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt taggcagtgg
     2161 ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc aggagttgat
     2221 tgcggcgggc gtcagtctgg tgagtttctc cggcgacaag ttgttaggcg ggccgcaggc
     2281 aggaattatt gttggtaaaa aagagatgat cgcccgcctg caaagccacc cgctgaagcg
     2341 tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc gtctttattt
     2401 acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga
     2461 ggtcattcaa atccaggcac aacgtttaca ggcccccctt gccgcacatt acggcgcgga
     2521 gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc tgccggttga
     2581 tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta gccaccttga
     2641 gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta tttatgacgg
     2701 acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg agatgttgtt
     2761 gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt attgcaggcg
     2821 attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat gaccatcgat
     2881 ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctggttttat cgacgtaccc
     2941 ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga tcacgcgctg
     3001 ttggtggtgg cgtgcgatga cggcgtgatg gcacagaccc gtgagcatct ggcgattttg
     3061 cagctgaccg gtaacccgat gctgacagtg gcgctgacca aagccgatcg cgtggacgaa
     3121 gcgcgtgtta atgaggttga acgccaggta aaggaggtgc tgcgggaata cggttttgct
     3181 gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag
     3241 catctgcttc agttgccgga acgcgagcac gccagccaac atagtttccg cctcgctatt
     3301 gaccgcgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc gttaagcggg
     3361 gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat gcgtgtacgt
     3421 gcgctgcatg cgcaaaacca gccaacagaa accgccaatg ccgggcagcg tatcgcgctt
     3481 aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct gcttgccgat
     3541 gtgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac accgctgacc
     3601 cagtggcagc cgctgcatat tcaccacgcc gccagccacg ttacgggacg cgtttcgctg
     3661 ctggaagata accttgccga gctggtgttc gacacgccgc tgtggctggc ggataacgat
     3721 cgcttagtgc tgcgcgatat ctcggcccgc aacacgctgg cgggggcgcg tgtggtgatg
     3781 cttaacccgc cgcgtcgcgg taaacgtaag ccggaatatc tgcaatggct ggcgtctctt
     3841 gcacgggcgc agagcgatgc cgatgcgtta tctgttcatc tggaacgcgg cgcggttaac
     3901 cttgcggatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga attgctgcaa
     3961 cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc cgctcgctgg
     4021 caacggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga tgaacctggg
     4081 cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc gctagtactg
     4141 ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg ttggctgcat
     4201 ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca aaaagcagag
     4261 ccgctgtttg gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac gggtaccgac
     4321 gagcaggcaa tgcgcctgac gctacgccag gcggcgcagc aaggaataat taccgcgatc
     4381 gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat gatccgcgat
     4441 ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc gcgatcgctt aggcgtaggc
     4501 cgaaagctgg caattcagat tctggaatat tttgaccgca ttggctttac gcgtcgtcgt
     4561 ggaaatgatc atttattgcg cgacgcatta tt
//
LOCUS       DAZFVO020000257         4590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFVO020000257 DAZFVO020000000
VERSION     DAZFVO020000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.259"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..127
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24220"
                     /old_locus_tag="pgaptmp_004844"
     rRNA            12..127
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24220"
                     /old_locus_tag="pgaptmp_004844"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            142..217
                     /locus_tag="ACHKNB_RS24225"
                     /old_locus_tag="pgaptmp_004845"
     tRNA            142..217
                     /locus_tag="ACHKNB_RS24225"
                     /old_locus_tag="pgaptmp_004845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Thr,seq:ggt)
     gene            257..372
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24230"
                     /old_locus_tag="pgaptmp_004846"
     rRNA            257..372
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24230"
                     /old_locus_tag="pgaptmp_004846"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(435..1193)
                     /gene="yhdZ"
                     /locus_tag="ACHKNB_RS24235"
                     /old_locus_tag="pgaptmp_004847"
     CDS             complement(435..1193)
                     /gene="yhdZ"
                     /locus_tag="ACHKNB_RS24235"
                     /old_locus_tag="pgaptmp_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGU4198208.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1201..2304)
                     /gene="yhdY"
                     /locus_tag="ACHKNB_RS24240"
                     /old_locus_tag="pgaptmp_004848"
     CDS             complement(1201..2304)
                     /gene="yhdY"
                     /locus_tag="ACHKNB_RS24240"
                     /old_locus_tag="pgaptmp_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4198209.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2314..3495)
                     /gene="yhdX"
                     /locus_tag="ACHKNB_RS24245"
                     /old_locus_tag="pgaptmp_004849"
     CDS             complement(2314..3495)
                     /gene="yhdX"
                     /locus_tag="ACHKNB_RS24245"
                     /old_locus_tag="pgaptmp_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGU4198210.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWFFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3563..4588)
                     /locus_tag="ACHKNB_RS24250"
                     /old_locus_tag="pgaptmp_004850"
     CDS             complement(3563..4588)
                     /locus_tag="ACHKNB_RS24250"
                     /old_locus_tag="pgaptmp_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGU4198211.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1238 a         1218 c         1114 g         1020 t
ORIGIN      
        1 aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg ccgaactcag
       61 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
      121 gccaggcatc aaatttagcg tgctgatatg gctcagttgg tagagcgcac ccttggtaag
      181 ggtgaggtcc ccagttcgac tctgggtatc agcaccactt tttaggttaa agttcggcag
      241 attagaaaag aatttgtctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
      301 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
      361 gaactgccag acatcaaatc aagcgaaagg ccatccgaaa ggatggcctt tttgcttttc
      421 gaactaacat tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta
      481 ggatgcgcaa aaaattcatc aggtgcagct tgttccacta tctccccacg atccataaaa
      541 attacccggt cagcgacggt acgtgcaaac cccatctcat gtgttacaca caacattgtc
      601 atacccgact gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg
      661 agcgccgacg ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca
      721 atggcaacgc gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg
      781 gcaattctca cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc
      841 ttgcgtaccc aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga
      901 ttgaaatgct gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct
      961 tcattaagtt cgatgccatc taccgcgatc cgtccctgtt gatgttcttc cagatgatta
     1021 atacaacgaa tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct
     1081 cccggttgca cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg
     1141 acgttttcca gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc
     1201 tcaatgcggt gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat
     1261 gctgaaacag aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc
     1321 caaccaggtg ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat
     1381 aatgatcacc aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat
     1441 taccagcttc aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc
     1501 cagcgactct gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac
     1561 ttccgcaaca tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc
     1621 gatactggtg ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt
     1681 aatcagcggt acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg
     1741 catatgggag cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc
     1801 aacggatgca ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc
     1861 cagaccaaaa aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca
     1921 ggccgcaata tagcgaccgc gatgcgggag tattttccag aacattggtg cgatcgacac
     1981 aagcccaatc agtaatgcga ggttaattcg ccagcgttgg tcgtgtgggt aaagcccata
     2041 cataaactga ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc
     2101 ggcacgcgtt gagccaaccc agttagcctg caaaaatgcc cagttcagca acggtgggat
     2161 cagttcccac atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa
     2221 cagatttttt cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg
     2281 ctgagacagc aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg
     2341 gttatagata ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt
     2401 catggctatc gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga
     2461 aaccatatcg ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata
     2521 ctggctggtt aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag
     2581 cgtaaccggg ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc
     2641 ctggatccca gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag
     2701 cgttaaggct gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag
     2761 ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc
     2821 aatgatcaac actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta
     2881 tgttttatta aagcggaata gcccaacaga aaggactata gccataacaa cagccaggat
     2941 aaacgcaata aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa
     3001 aaaggccaga tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc
     3061 aaagaaccag aagaagattt gcagcagcgg gggaatatta cggaagatct caatataaat
     3121 tgtggatagt tttcgtagca gccagttatc cgaaagtctc gccagaccga taaagaagcc
     3181 taaaacagaa gcgaacacaa tacacaatgc agaaaccagt agcgtattga gtaagccgac
     3241 aataaaaacg cgtccgtagg tgtcgccctg ctggtaatcg atcaaatgct ggacaatacc
     3301 gaagccagcg ccgcgatcca gaaaggcaaa acctgaagta atgccacgat tattgagatt
     3361 cgttacagtg ttgtgaaata accaaccaac aatgccgaca acagcaacaa cggcaaggat
     3421 ctggaataac caggcgcgaa ccgtagggtt ggcaaaagag agtgagcctt taacggttga
     3481 gcggcgatga gacatagaaa cctcggaaag cataacgctg aagagagcgc cgccgtagcg
     3541 acgcccgtta cctcacaccg acttaacgca cgggcggcgc gtactgaata ccgccgttat
     3601 tccagagatt attttgccca cgtttaattt tcagcgggct ttctgaacct acgttacgct
     3661 cgaaaatttc cgagtagtta cccacctgtt tgatgatgtt ataggcccat ttattatcca
     3721 gcttcagatc cttgccgtaa tcgccttctt tacccagcag atgtgccata tcaggcgttg
     3781 ctggattagc cgctttttca tcgacgttct gggaattgat gcccatctct tcagcattca
     3841 gcatggcgaa aagcgtccag cgtacaatcg agaaccattc atcatcgcca cgacgaacta
     3901 ccggcccaag cggttcttta gagataacct ccggtaagac aatccattca gctgggttgc
     3961 ttaatttgat gcgcagggca tacagttgcg actgatccga ggccagcgta tcgcagcgac
     4021 cagattccag tgccttcgct gattcgtcag agcgatcgaa agtcaccggt gtgtacttca
     4081 tattgtttgc cttgaaatag tcggcaacgt tgagttcggt atcagtaccc gcctgaatac
     4141 agacggtagc gccatccagt tctttcgcgc tttttagccc cgctttatcg tgcgtcagga
     4201 agccaatgcc gtcgtaataa gtgacgccgg taaatgccat tcccatcccg gcatcgcgag
     4261 atgaagtcca ggtcgtatta cgggagagca aatccacttc ccctgactgt aaagcggtga
     4321 agcgttcttt tgctgtgagt ggggtatatt tcactttcgt gtcgtcacca aatacagcag
     4381 cggcaacacc acgacaaaca tcaacatcaa tacctgaaaa cttaccgtct gcatcggcat
     4441 aagagaaccc aggtaatcca tcactgatcc cgcattgcac aaaacctttt ttctgaacgg
     4501 catcaagcgt cgcgccagca tgcgcctgat ttgcaacggc aagcagcacg ctggcggcag
     4561 ccagtgtggc tatcatcatc tttttcataa
//
LOCUS       DAZFVO020000258         4490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFVO020000258 DAZFVO020000000
VERSION     DAZFVO020000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.260"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..309)
                     /locus_tag="ACHKNB_RS24255"
                     /old_locus_tag="pgaptmp_004851"
     CDS             complement(<1..309)
                     /locus_tag="ACHKNB_RS24255"
                     /old_locus_tag="pgaptmp_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4198212.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     I"
     gene            complement(424..499)
                     /locus_tag="ACHKNB_RS24260"
                     /old_locus_tag="pgaptmp_004852"
     tRNA            complement(424..499)
                     /locus_tag="ACHKNB_RS24260"
                     /old_locus_tag="pgaptmp_004852"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(464..466),aa:Thr,seq:ggt)
     gene            complement(506..580)
                     /locus_tag="ACHKNB_RS24265"
                     /old_locus_tag="pgaptmp_004853"
     tRNA            complement(506..580)
                     /locus_tag="ACHKNB_RS24265"
                     /old_locus_tag="pgaptmp_004853"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(545..547),aa:Gly,seq:tcc)
     gene            complement(697..781)
                     /locus_tag="ACHKNB_RS24270"
                     /old_locus_tag="pgaptmp_004854"
     tRNA            complement(697..781)
                     /locus_tag="ACHKNB_RS24270"
                     /old_locus_tag="pgaptmp_004854"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Tyr,seq:gta)
     gene            complement(790..865)
                     /locus_tag="ACHKNB_RS24275"
                     /old_locus_tag="pgaptmp_004855"
     tRNA            complement(790..865)
                     /locus_tag="ACHKNB_RS24275"
                     /old_locus_tag="pgaptmp_004855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(830..832),aa:Thr,seq:tgt)
     gene            1227..2177
                     /gene="coaA"
                     /locus_tag="ACHKNB_RS24280"
                     /old_locus_tag="pgaptmp_004856"
     CDS             1227..2177
                     /gene="coaA"
                     /locus_tag="ACHKNB_RS24280"
                     /old_locus_tag="pgaptmp_004856"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGU4198213.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2206..3171)
                     /gene="birA"
                     /locus_tag="ACHKNB_RS24285"
                     /old_locus_tag="pgaptmp_004857"
     CDS             complement(2206..3171)
                     /gene="birA"
                     /locus_tag="ACHKNB_RS24285"
                     /old_locus_tag="pgaptmp_004857"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGU4198214.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3168..4196)
                     /gene="murB"
                     /locus_tag="ACHKNB_RS24290"
                     /old_locus_tag="pgaptmp_004858"
     CDS             complement(3168..4196)
                     /gene="murB"
                     /locus_tag="ACHKNB_RS24290"
                     /old_locus_tag="pgaptmp_004858"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGU4198215.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTHLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1139 a         1126 c         1018 g         1207 t
ORIGIN      
        1 gatcgcgccg tccatctgag cagcaccggt gatcatgttt ttaacatagt cggcgtgccc
       61 cgggcagtct acgtgtgcgt agtgacgggt cggggtgtcg tattcaacgt gagaagtgtt
      121 gatggtgata ccacgagctt tttcttccgg cgcgttatcg atctggtcga atgcgcgagc
      181 agcaccgccg taggttttag ccagtacggt agtgattgca gcggtcagcg ttgttttacc
      241 atggtcaacg tggccgatag taccgacgtt aacgtgcggt tttgtacgtt caaacttttc
      301 tttagacatc gattgtccct ctaagacacg gataaatcgg tgatatcacc acatcaacca
      361 ggcaacatgc ccgacttgtt gaatgcaata aacagaagga aaaaacaggg aggagaaaag
      421 aagtggtgct gataggcaga ttcgaactgc cgacctcacc cttaccaagg gtgcgctcta
      481 ccaactgagc tatatcagca catcttggag cgggcagcgg gaatcgaacc cgcatcatca
      541 gcttggaagg ctgaggtaat agccattata cgatgcccgc atcctggaac tcggctacct
      601 gattttcatt ctgcactgaa tatcgagaga agctctcttt attcgagccg gtaagcgaac
      661 ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt ggtgggggaa ggattcgaac
      721 cttcgaagtc tgtgacggca gatttacagt ctgctccctt tggccgctcg ggaaccccac
      781 cggacttgat ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt
      841 gctctaccta ctgagctaag tcggcatcaa gtagcgcgca ttctatggag acatgcgagt
      901 tcatgcaact aaaaaattgc ataatttgtt ttattggtca cattttatgc gacacgatga
      961 agaaacagcc gaacggaggg cagattagca cactttttca acatcattgt gctcaacaat
     1021 gcgctcctgc taaaccataa ttctttttat cagatggaat atctgtcaca ttgcttttca
     1081 acgatagctt cctggcagag attttttctt attattcctc cccatctggt gttaccctcc
     1141 tgcccattaa cccattcaac agaactgtga cgcgccatgg caaatatcgc tttgccgata
     1201 gagctatgac cgccagaaac atgcttatga gtataaaaga gcaaacgtta atgacgcctt
     1261 acctacagtt tgaccgcaac cagtgggcag ctctgcgtga ttccgtacct atgacgttat
     1321 cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga tctctcgtta gaagaagttg
     1381 ccgagatcta tttacctttg tcacgtttgc tgaacttcta tataagctcg aatctgcgcc
     1441 gtcaggcagt tctggaacag tttcttggta ctaacgggca acgcattcct tacattatca
     1501 gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc ccgtgtattg caggcgctat
     1561 taagccgttg gccggaacat cgtcgtgttg aactgatcac tacagatggc ttccttcacc
     1621 ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa aggcttcccg gaatcgtatg
     1681 atatgcatcg cctggtgaag tttgtttccg atctcaaatc cggcgtgcca aacgttacag
     1741 cccctgttta ctcgcatctt atttatgatg tgatcccgga tggagataaa acggttgttc
     1801 agcctgatat tttaattctt gaagggttaa atgtcttaca gagcgggatg gattatccac
     1861 acgatccaca tcatgtattt gtttctgatt ttgtcgattt ttcgatatat gttgatgcac
     1921 cggaagactt acttcagacg tggtatatca accgttttct gaaattccgc gaaggggctt
     1981 ttaccgaccc ggattcctat tttcataact acgcgaaatt aactaaagaa gaagcgatta
     2041 agactgccat gacattgtgg aaagagatca actggctgaa cttaaagcaa aatattctac
     2101 ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc taatcatgcg gtagaagagg
     2161 tcagactacg caaataattt tcaggggagc taatgctccc ctttcttatt tttctgcact
     2221 acgcagggat atttcaccgc ccatccaggg ttttattatt ccatcctgct caagcaataa
     2281 agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt tctttatcac caatgataag
     2341 tttcactggg cgattaataa aattatccag cttttcccag cgcgaaagat aaggtgccaa
     2401 tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca cgtattagca tggccgccaa
     2461 cgtattacga tcgagattga tccccgcttc ctgcagcgtg atccacccct gattaacgac
     2521 actctcttca acacgacgca ttgccatgtt gatcccggct ccaatgacta tttgcgccgc
     2581 atcgccggtt ttgccagtca gctccacaag aatgcctgcc agcttgcgat cctgcagata
     2641 gaggtcatta ggccatttaa cacgaacttt atctgcaccc agcttgcgta atacttccgc
     2701 catcacgata ccgataacca gacttaaacc aatagccgcc gccgggcctt gttccaaacg
     2761 ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa aaccatttcc gaccccggcg
     2821 accacggcca gcctgctggt attctgcaac gcaggcatcg cccgatttaa gctctccgat
     2881 acgatcaaga aggtactgat tcgtggagtc aatcactggc agcacggcta cactaccgcc
     2941 atccagctga ctcaatatct cttcagcatt aagtaactgg ataggttcag gcaggctgta
     3001 tcctttaccc ggaacggtaa agacatcaac gccccagtca cgcagtgtct gaatgtgttt
     3061 attaatagcc gcccggctca ttcccagcgt ttcacccaac tgctcgccag agtgaaattc
     3121 accgttcgct aacagggcaa tcaatttcag tggcacggtg ttatccttca tgaaattgtc
     3181 tccactgcgc tcacttcacc tgatgcacca ataaagcgga cttcaggctc aagccagaca
     3241 ttaaattttt cacccacttt ttgtcttaca tggtgtgcca gttgcaccac atcttcgctt
     3301 tttgcattgt cttcattaat gagaactaac gcctgttgac ggtgcaccgc agccccaccc
     3361 atttgcatcc cttttagctg gcactgatca ataagccaac ctgctgccag ttttactgaa
     3421 ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg ccagtaatgc tttagccgtt
     3481 tcggcagata caacagggtt tttgaagaaa ctaccggcat tgccattcac ttttggatca
     3541 gggagtttgg tggtgcgcat atgacacacc gcattaaata cttgctgtgg cgttactgtt
     3601 gtaggatcca gatgagttaa gtcaccatac gttagtacag gttgccactc ttttggcaga
     3661 cgcagaccta cggctacaat ggcgaagcga tcctggtatt catgtttaaa aatactgtcg
     3721 cgatagccaa aacggcactc tttggcagtt aagcgcactt gcttgcctgt cgccagttca
     3781 acacaatcaa cataagcgca aactcgctgt aattctacgc cataagcacc aatattctgg
     3841 ataggtgatg agccgacaca accaggaatt aatgccagat tttccagacc aggcatacct
     3901 tcctgcaaag tgtattttac caggcgatgc cagttttctc cggctcctac atgtaaatac
     3961 cacgcatcag gttcatcatg aatttcgata cctttgatcc ggttgatgat caccgtgccg
     4021 cgatagtctt ccagaaaaag tacattactt ccttcaccca gaataagaac gggttgtcct
     4081 tctgcggttg catactgcca ggcattgagt aattgttgtt cgtcttcggc acatacaatg
     4141 tgctgagcat tatgatcaat gccaaatgtg ttccagggtt ttaaggagtg gttcatagct
     4201 gctttcctga tgcaaaaacg aggctagttt accgtatctg tggggggatg gcttgtagat
     4261 atgacgacag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     4321 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     4381 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     4441 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
//
LOCUS       DAZFVO020000259         4424 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFVO020000259 DAZFVO020000000
VERSION     DAZFVO020000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.261"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            44..586
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS24295"
                     /old_locus_tag="pgaptmp_004859"
     CDS             44..586
                     /gene="fimA"
                     /locus_tag="ACHKNB_RS24295"
                     /old_locus_tag="pgaptmp_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HGU4198216.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRIDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            806..1498
                     /gene="fimC"
                     /locus_tag="ACHKNB_RS24300"
                     /old_locus_tag="pgaptmp_004860"
     CDS             806..1498
                     /gene="fimC"
                     /locus_tag="ACHKNB_RS24300"
                     /old_locus_tag="pgaptmp_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HGU4198217.1"
                     /translation="MMTKIKLLMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLKLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1529..4138
                     /gene="sfmD"
                     /locus_tag="ACHKNB_RS24305"
                     /old_locus_tag="pgaptmp_004861"
     CDS             1529..4138
                     /gene="sfmD"
                     /locus_tag="ACHKNB_RS24305"
                     /old_locus_tag="pgaptmp_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HGU4198218.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMS
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4151..>4424
                     /locus_tag="ACHKNB_RS24310"
                     /old_locus_tag="pgaptmp_004862"
     CDS             4151..>4424
                     /locus_tag="ACHKNB_RS24310"
                     /old_locus_tag="pgaptmp_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198219.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQET"
BASE COUNT         1189 a          956 c         1045 g         1234 t
ORIGIN      
        1 atatacggat gtggagtcga taaatgagat tgaaggaata tatatgaaat taagatttat
       61 ttcgtctgcg ctggctgccg cactattcgc cgctacgggt agttatgctg ccgttgtaga
      121 tggcggtaca attcactttg aaggcgaact ggtgaatgct gcctgttcag tgaatactga
      181 ctcggcagac caggttgtca cactcggtca atatcgtatc gatattttca atgctgttgg
      241 taatacctct gcattaattc cattcaccat tcagttgaac gactgcgatc ctgttgttgc
      301 cgctaatgct gccgttgcat tttctggtca ggctgatgca atcaatgata atttattggc
      361 cattgcatcc agtaccaata caacaacagc aacgggtgtc ggtattgaaa tacttgataa
      421 tacatccgca attctcaaac ctgatgggaa tagcttctca accaaccaga acttgatccc
      481 cgggaccaac gttcttcatt tttctgcacg ttataaaggc accggtacaa gtgcatcagc
      541 agggcaagca aatgctgacg cgacttttat tatgagatac gaataatcaa aaccacgttg
      601 ttttgaatta tatatcacgt cttataacaa agtaatgtac cggttgtctg aagcggtatg
      661 gtggcaatgt aaatcgaaat catgttcact ttgtatcatg ccgctttatt aaatgaaaag
      721 ggaatgatgt gttgtaagaa accaaagcaa tcatttcttt atatttctta tttttgccgt
      781 caggaataca caaggcgtat taactatgat gactaaaata aagttattga tgctcattat
      841 attttattta atcatttcgg ccagcaccca tgctgccgga gggatcgcat taggtgccac
      901 gcgtattatt tatcccgctg atgctcaaca gactgcggta tggattagaa atagccatac
      961 caatgagcgc tttctggtca attcgtggat tgaaaacagc agcggtgtaa aagaaaagtc
     1021 attcatcatt acaccgccac tgtttgttag tgaacccaaa agcgaaaata ccttgcgtat
     1081 tatttacacc ggtccaccgc tggcggcaga tcgtgagtct ctgttctgga tgaatgttaa
     1141 gacgatccct tcggtagata aaaatgcatt gaacggcagg aatgttttga aactggcgat
     1201 tttatcgcgc atgaaattat ttctccgtcc aattcaatta caagaattac ccgcagaagc
     1261 gccggacaca ctcaagtttt cgcgatccgg taactatatc aatgttcata atccctcacc
     1321 tttttatgtc accctggtta acttacaagt gggcagccaa aagttgggga atgctatggc
     1381 tgcacccaga gttaattcac aaattccctt accctcagga gtgcagggaa agctgaaatt
     1441 tcagaccgtt aatgattatg gttcagtaac cccggtcaga gaagtgaact taaactaacc
     1501 gaatcatctg acaatatcag agctaattat gaaaataccc actactacgg atattccgca
     1561 gaggtatacc tggtgtctgg ccggaatttg ttattcatct cttgccattt taccctcctt
     1621 tttaagctat gcggaaagtt atttcaaccc ggcattttta ttagagaatg gcacatccgt
     1681 tgctgattta tcgcgctttg agagaggcaa tcatcaacct gcgggagtgt atcgggtgga
     1741 tctctggcgt aatgatgagt tcattggttc acaggatatc gtatttgaat cgacaacaga
     1801 aaatacaggt gataaatcag gtgggttaat gtcctgtttt aaccaggtac tccttgaacg
     1861 aattggcctt aatagcagtg catttcccga gttagcccag cagcaaaaca ataaatgcat
     1921 caatttactg aaagctgtac ctgatgccac aattaacttt gattttgcag cgatgcgcct
     1981 gaacatcact attcctcaga tagcgttgtt gagtagcgct cacggttaca ttccgcctga
     2041 agagtgggat gaaggtattc ctgctttact cctgaattat aatttcaccg gtaacagagg
     2101 taatggtaac gatagctatt tttttagtga gctcagcggg attaatattg gcccgtggcg
     2161 tttacgcaac aatggttcct ggaactattt tcgcggaaat ggatatcatt cagaacagtg
     2221 gaataatatt ggcacctggg tacagcgcgc cattattccg ctgaaaagtg aactggtaat
     2281 gggagacggc aatacaggaa gtgatatttt cgatggtgtt ggatttcgtg gtatacggct
     2341 ttactcttcc gataatatgt atcctgatag ccagcaaggg tttgccccaa cggtacgtgg
     2401 gattgcccgt acggcggccc agctaacgat tcggcaaaat ggttttatta tctatcaaag
     2461 ctatgtttcc cccggcgctt ttgaaattac agatttgcac ccgacatctt caaatggcga
     2521 tctggatgtc accatcgacg agcgcgatgg caatcagcag aattacacaa ttccgtattc
     2581 aacagtgccg attttacaac gcgaagggcg tttcaaattt gacctgacgg cgggcgattt
     2641 tcgtagcggt aatagtcagc aatcatcacc tttctttttt cagggcacgg cactcggcgg
     2701 tttaccacag gaatttactg cctacggcgg gacgcaatta tctgccaatt acaccgcctt
     2761 tttattagga ctggggcgca atctcggaaa ctggggggca gtgtcgctgg atgtgaccca
     2821 tgcgcgcagt cagttagccg acgacagtcg tcatgagggg gattccattc gcttcctcta
     2881 tgcgaaatcg atgaacacct tcggcaccaa ttttcagtta atgggttacc gctattcgac
     2941 acaaggtttt tatacccttg atgatgttgc gtatcgtcga atggaggggt acgaatatga
     3001 ttacgattac gacggtgagc atcgggatga actgataatc gtgaattacc acaatttacg
     3061 ctttagccgt aaagaccgtt tgcagttaaa tatttcacaa tcacttaatg actttggctc
     3121 gctttatatc tctggtaccc atcaaaaata ctggaatact tcggattcag atacctggta
     3181 tcaggtgggg tataccagca gctgggttgg catcagttat tcgctctcat tttcgtggaa
     3241 tgaatctgta gggatccccg ataacgaacg tattgtcgga cttaatgttt cagtgccttt
     3301 caatgttctg accaaacgtc gctacacccg ggaaaatgcg ctcgaccgcg cttatgcctc
     3361 ctttaacgcc aaccgtaaca gcaacgggca aaatagctgg ctggcaggtg taggtgggac
     3421 cttactagaa ggccacaacc tgagttatca cgtaagccag ggtgatacct cgaataatgg
     3481 gtatacgggc agcgccacgg caaactggca ggccgcttac ggtacgctgg gggtcgggta
     3541 taactacgac cgcgatcaac atgacgttaa ctggcagctg tctggcggtg tggtcgggca
     3601 cgaaaatggc ataacgctga gccagccttt aggggatacc aatgttttga ttaaagcgcc
     3661 tggcgcaggc ggtgtacgca ttgaaaatca aactggcatt ttaaccgact ggcgcggcta
     3721 tgcggtgatg ccgtatgcca cggtttatcg gtataaccgt atcgcgcttg ataccaatac
     3781 gatggggaat tccatcgatg ttgaaaaaaa tattagcagc gttgtgccga cgcaaggcgc
     3841 gttggttcgt gccaattttg atacccgcat aggcgtgcgg gcgctcatta ccgttaccca
     3901 gggcggaaaa ccggtgccgt ttggatcact ggtacgggaa aacagtaccg gaataaccag
     3961 tatggtgggt gatgacgggc aagtttattt aagtggtgcg ccattgtctg gtgaattact
     4021 ggttcagtgg ggagacggcg cgaactcacg ctgcattgcg cactatgtat tgccgaagca
     4081 aagcttacag caagccgtca ctgttatttc ggcagtttgc acacatcctg gctcataaag
     4141 gaaattatca atgaagataa tctgcagatt attattggcg atggcatgtt tgtgtctggc
     4201 aaacatatcc tgggctactg tttgtgcaaa tagtactggc gtagcagaag atgaacacta
     4261 tgatctctca aatatcttta atagcaccaa taaccagcca gggcagattg ttgttttacc
     4321 ggaaaaatcc ggctgggtag gtgtgtcagc aatttgtcca cccggcacgc tggtgaatta
     4381 tacataccgt agttatgtca ccaactttat tgttcaggaa acta
//
LOCUS       DAZFVO020000260         4405 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFVO020000260 DAZFVO020000000
VERSION     DAZFVO020000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.262"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1153
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS24315"
                     /old_locus_tag="pgaptmp_004863"
     CDS             <1..1153
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS24315"
                     /old_locus_tag="pgaptmp_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4198220.1"
                     /translation="TVALSAVAAAVIFAAGAQAAEVYNKDGNKLDVYGRATALHYFSS
                     DKGDDGDKTYARLGFKGETQINDQLTGFGQWEYQFSGNKAESEGAAGNKTRLAFAGLK
                     FGEFGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNTDFFG
                     LVDGLNFAAQYQGKNDRGDLRKANGDGHGFSASYEYEGFGIVAAYINADRTNNQEKGL
                     NGTLTIEQTDPLTGKVTEKQIVVDSGSVAKGKHAEMWGVGLKYDADNLYLATTYSETQ
                     NLTTFGDKGVADKAQNFEAVLQYQFDFGLRPSLAFLQSRAQDVIVGGKNYGDQDLVKY
                     VDVGAKYYFNKNMSTYVDYKINLVDENKFTRDAGIATDDIVAVGMTYQF"
     gene            1350..2048
                     /locus_tag="ACHKNB_RS24320"
                     /old_locus_tag="pgaptmp_004864"
     CDS             1350..2048
                     /locus_tag="ACHKNB_RS24320"
                     /old_locus_tag="pgaptmp_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4198221.1"
                     /translation="MQEINEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            2059..2802
                     /locus_tag="ACHKNB_RS24325"
                     /old_locus_tag="pgaptmp_004865"
     CDS             2059..2802
                     /locus_tag="ACHKNB_RS24325"
                     /old_locus_tag="pgaptmp_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HGU4198222.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVITTTEGERYLPCVNISAAPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFAHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWCNGQNLYLDNMEAT
                     GFYRVPLSSALPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            2799..3380
                     /locus_tag="ACHKNB_RS24330"
                     /old_locus_tag="pgaptmp_004866"
     CDS             2799..3380
                     /locus_tag="ACHKNB_RS24330"
                     /old_locus_tag="pgaptmp_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4198223.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
     gene            3723..>4405
                     /locus_tag="ACHKNB_RS24335"
                     /old_locus_tag="pgaptmp_004867"
     CDS             3723..>4405
                     /locus_tag="ACHKNB_RS24335"
                     /old_locus_tag="pgaptmp_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198224.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFG"
BASE COUNT         1041 a          988 c         1317 g         1059 t
ORIGIN      
        1 gacagtggca ctttcagctg tggcagcagc tgtaattttt gcagcggggg cgcaggctgc
       61 agaagtatac aataaagatg gcaataaact tgatgtgtat gggcgtgcaa ctgcactcca
      121 ttatttctcc agtgataagg gggatgatgg agataagaca tacgcacgtc tgggctttaa
      181 aggtgaaact cagattaatg accagttgac tggttttggt cagtgggaat atcagttctc
      241 tggtaataaa gctgaatcag agggtgctgc gggtaataaa acccgtcttg catttgcagg
      301 tctgaagttt ggtgagtttg gcaccattga ttatggtcgt aactatggta ttgcgtacga
      361 tgttggttct tatactgacg tattaccaga gtttggcggt gatggctgga cccagaccga
      421 taacttcatg acagcccgta cttccggggt attaacctat cgtaatacag atttctttgg
      481 gcttgtcgat ggcctgaatt ttgcggcaca gtaccagggt aaaaatgatc ggggtgatct
      541 ccggaaagct aatggtgatg gtcacggttt ctcagccagt tatgaatatg aaggttttgg
      601 tatcgttgcc gcatatatta atgcggaccg aaccaataat caggaaaaag gtctgaatgg
      661 tactctgacg attgaacaaa ctgatccttt aaccggtaaa gtgactgaaa agcagatcgt
      721 tgttgattcc ggtagtgttg caaaaggtaa acacgctgag atgtggggag tcggactaaa
      781 atacgatgct gacaaccttt acctagctac aacatattcc gaaactcaga atctgacgac
      841 ctttggtgat aaaggagttg cagataaagc gcaaaacttt gaagcggtac tgcagtatca
      901 gtttgatttc ggtctgcgtc catctctggc attcctgcag tctcgtgctc aggatgtcat
      961 tgtaggtggt aaaaactatg gtgaccagga tttggttaag tatgttgacg taggtgcgaa
     1021 atattacttc aataaaaata tgtccaccta tgttgattat aaaatcaatc tggttgatga
     1081 gaataagttt accagggatg cgggcattgc aacggatgat atcgttgctg ttggtatgac
     1141 ttatcagttc tgatgattac tggtaaataa atacaggcca tctgccctca cagatggcct
     1201 gtatcagtgg aagttatatt tccactggtc actgcgatca gctattagct gatttaattt
     1261 tcattttatt accttcatcg tttttgattt tttaattgaa ctattttatc aggggcgcat
     1321 tgcgcccctt tttttatggg tggatgttta tgcaggagat caacgaagaa agcctgaatg
     1381 agtcggttaa atcagagcag tcaccgcggg tggtactctg ggaaattgac ctgacggtgc
     1441 agggtggtga gcggtatttt ttctgtaatg agctgaatga aaaaggggag ccggtcacct
     1501 ggcaggggcg gaagtaccag gcatacccga ttgacggcag cggctttgag atgaacggga
     1561 aagggagcag tgcccgcccg tcactgacgg tgtcgaatct gttcgggctg gttaccggga
     1621 tggcggagga tttgcagagc ctggtggggg ccacggtggt tcgtcgccgg gtgtatgcgc
     1681 gttttctgga tgcggtgaat tttgtggcag gcaatccgga ggctgacccg gagcaggagc
     1741 tgacggaccg ctgggtggtg gagcagatgt cgcagctcac agccatgacg gcctcgtttg
     1801 tgctggcgac accgacggag acggacggtg cgttgtttcc cggccgcatc atgctggcga
     1861 acacctgtat gtggacctac cgctctgatg agtgtggtta cacgggcggg gcggtggcgg
     1921 atgagttcga taaacccacc accgatatcc gtaaggacag atgcagcaag tgcatgcgcg
     1981 ggtgtgaact gcgcaggaat gtcggcaatt ttggcggttt cctttccatt aataaacttt
     2041 cgcagtaaat cccggtttat gacacagact gaatcagcga ttctggcgca tacccggcgg
     2101 tgtgcgcctg cggagtcgtg cggcttcgtg ataaccacga cggagggcga gcggtacctg
     2161 ccctgtgtga atatctccgc agcgccggag gcgtattttc gtattgcccc ggaagactgg
     2221 ctgcgggcgg agatgcaggg tgagattgtg gcgctggttc acagtcatcc cggtggtctg
     2281 ccctggctga gtgaggcaga ccggcgactg cagataaaaa gtgccctgcc ctggtggctg
     2341 gtctgccggg gtgacattca caaattccgc tgcgttccgc acctgaccgg acggcgcttt
     2401 gcgcacgggg tgacggactg ttacacgctg ttccgggatg cataccatct ggcaggcatt
     2461 gatatgccgg attttcaccg tgaggatgac tggtggtgca acggtcagaa cctttacctg
     2521 gacaatatgg aggcaacggg cttttaccgg gtgcccctgt cctctgcact gccgggcgat
     2581 atcctgctgt gctgcttcgg cgcatcggtg gctaatcatg ccgccattta ctgcggcaac
     2641 ggtgaactgc ttcaccatct gcctgaacaa ctgagtaaac gggagaggta ttccgaaaaa
     2701 tggcaacgac gaacgcattc tgtctggcgt caccgccact ggcacgcatc tgccttcacg
     2761 gggatttaca acgatttggc cgccgcttca gcctgtatgt gaacacggcg gcggaagcca
     2821 tccgcgccct gtcgctgcag gtgccgggat tccgccgtca gatgaacgaa ggctggtacc
     2881 agatacgcat tgccggtgag gatacggcac cggaggcggt gtatgcccgc cttcacgaac
     2941 cactgggtga gggaacggtt atccatattg tgccgcgact ggccggagcc ggaaagggcg
     3001 gactacagat tgtgctgggg gcagctgcca ttgtggggtc tttcttcacg gccggagcat
     3061 caatggcgtt atggggttca gccctggcag ccggtggttt ttctgccacc acgatgctgt
     3121 tttcactggg tgccagcatg atactgggcg gtgtggccca gatgctggcc ccgaaggcaa
     3181 aaacaccgga ttaccgcgca acggataacg gcagacagaa cacgtatttt tcctcactgg
     3241 acaacatgat tgcccagggc aacccgatgc cggtgcccta cggtgaaatg ctggttggtt
     3301 cacgacgtat ctctcaggac atcagcaccc gtgatgaagg cggagacgga aaggtggtgg
     3361 ttattggtcg acagggataa aacataaaaa aatcccgcag tgatcgcgga gctgcgggga
     3421 cagacaaatg aagattaatg ttaaggagtt gtttttgtta ctcgggcaaa aaaacactaa
     3481 cgcagcgaaa ttataagcgc cacagtcagt gtgtgaaaat gtgaagatat tcagaatttt
     3541 tatgccatta ccggttttaa ccaacaggat tatcggtggg catgaaagaa aaccccggta
     3601 tctgctgata ccggggtttc tgtttagcat ggcagaaatg tgtttcatgc ttttcgggcg
     3661 aaggatatcc gacttctgta cggaatggca agtggcggtt aatttattca ggggaaggct
     3721 gtatgggaaa aggtggcggt aaggcacaca cgcctcgtga ggcgaaggat aatctcaaat
     3781 ccacgcagat gatgagcgtg attgatgcga ttggtgaggg acccattgaa ggtccggtga
     3841 aaggcctgca gagtattctg gtgaacaaaa ccccgctgac ggacacggac ggtaatcccg
     3901 tgatacacgg tgtgaccgcg gtctggcgcg ccggggagca ggagcagaca ccaccggaag
     3961 gctttgagtc ctccggagct gaaactgtac tgggtgtgga agtgacgaag gcaaaaccgg
     4021 tgacacgcac cattacgtca gcgaacattg accgcctgcg ggtgaccttc ggggtgcagt
     4081 cactggtgga gaccacgtca aagggtgacc gtaatccgac gtctgtccgc ctgctgattc
     4141 agcttgagcg tggtggtaaa tggatgacgg aaaaggatgt caccattaac ggcaagacca
     4201 cctcacagtt ccttgcgtcg gtgattctgg ataatctgcc tccccgcccc tttaacatcc
     4261 ggatggtcag ggagacggcg gacagcacca cggaccagct gcagaataag acgctctggt
     4321 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc attgtggggc
     4381 tgcaggtgga tgcggagcag ttcgg
//
LOCUS       DAZFVO020000261         4338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFVO020000261 DAZFVO020000000
VERSION     DAZFVO020000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.263"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            67..351
                     /locus_tag="ACHKNB_RS24340"
                     /old_locus_tag="pgaptmp_004868"
     CDS             67..351
                     /locus_tag="ACHKNB_RS24340"
                     /old_locus_tag="pgaptmp_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HGU4198225.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            404..1714
                     /locus_tag="ACHKNB_RS24345"
                     /old_locus_tag="pgaptmp_004869"
     CDS             404..1714
                     /locus_tag="ACHKNB_RS24345"
                     /old_locus_tag="pgaptmp_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4198226.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSQKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLSRQHQITVQLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            1711..2289
                     /gene="wrbA"
                     /locus_tag="ACHKNB_RS24350"
                     /old_locus_tag="pgaptmp_004870"
     CDS             1711..2289
                     /gene="wrbA"
                     /locus_tag="ACHKNB_RS24350"
                     /old_locus_tag="pgaptmp_004870"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HGU4198227.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVTQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            2310..2537
                     /locus_tag="ACHKNB_RS24355"
                     /old_locus_tag="pgaptmp_004871"
     CDS             2310..2537
                     /locus_tag="ACHKNB_RS24355"
                     /old_locus_tag="pgaptmp_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HGU4198228.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2575..3816)
                     /gene="agp"
                     /locus_tag="ACHKNB_RS24360"
                     /old_locus_tag="pgaptmp_004872"
     CDS             complement(2575..3816)
                     /gene="agp"
                     /locus_tag="ACHKNB_RS24360"
                     /old_locus_tag="pgaptmp_004872"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HGU4198229.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGQLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDKQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4110..>4338)
                     /locus_tag="ACHKNB_RS24365"
                     /old_locus_tag="pgaptmp_004873"
     CDS             complement(4110..>4338)
                     /locus_tag="ACHKNB_RS24365"
                     /old_locus_tag="pgaptmp_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198230.1"
                     /translation="LSDSICSLFIVNSNMASISLGNESFSTDDDLEYGYLINAGNHYD
                     VYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT         1200 a         1052 c         1027 g         1059 t
ORIGIN      
        1 cgccgtacat caatcaactg acaaccgcct tcgggcggtt ttttcttaca catgcagagg
       61 caacttatgc gcgagttagt aaaccaacat aaccatggca ttcagccagt catcacacct
      121 gttgtacaga taaatgcgaa tgaatgggta acactggagc ttttaatggc tgtaacaggc
      181 ctaagaaaag gaacaatatt acgcgccagg gacagtgcgt ggatgaacgg cagagaatat
      241 aaacaaattg cccccgacgg aacgccaaag aaaaacagcg aatgtctcta tcatcttcct
      301 accatcaaca cttggatcaa aaaccaaccc ttaccatctc aggatgttta attcttgtcc
      361 ataagagtat aacctgagcg tgctcttgga cgcaggagga acaatggcga attcagccta
      421 tccagccggc gttgaaaatc acggaggaaa actccgaata acgtttaagt acaggggtaa
      481 acgagtgcgc gaaaatcttc gcgtgcccga tactccgaaa aacagaaaga tcgctggtga
      541 gttaagggct tctgtctgct ttgcaatcag aacgggaacg tttgattatg ccgagcgatt
      601 ccctgactca cctaacctga agctatttgg cctggtaaaa aaagatatca ccgtcggtga
      661 actggcacag aaatggctta ctctgaaagc aatggaaatc agtagtaacg ccttaaatcg
      721 ttatcaatca gtgatgaaaa atatgctacc gagacttggt cctggcaggc tggcgtcatc
      781 gattacaaaa gaagatctgc tgtttatcag gaaagattta ctgaccgggg aaaagggaag
      841 caggaaaacc agcacgtccc aaaaaggaag aaccgtaccc acagtgaact attacatgac
      901 aacaacagcc ggaatgttca gctttgccgc cgaaaacggg tatctggaga aaaacccgtt
      961 taattcaata acaccgctga ggaaatcaaa accagtgccg gatccactga ccagagatga
     1021 gtttagccgt ctcattgatg cctgccatca tcaacagacc aaaaacctct ggacagtggc
     1081 tgtttttaca gggatgcgac acggtgaaat tgccgcactt gcatgggagg atatcgacct
     1141 gaaagctggc acgataacag tgcgaagaaa ttttacaaaa ataggtgatt ttacgctacc
     1201 aaagactgac gcaggcacta accgggttat acatcttctg gcaccagcaa ttgaagcact
     1261 taaaaaccag gcgatgctta ctcgtcttag caggcagcat cagatcactg ttcaattacg
     1321 cgagtacgga agaacaattt tgcacgagtg cacttttgtt ttctgtccgc aaatcgttcg
     1381 caagaatcac aaggctggta ttaactacgc ggtaagctcc atcggagcga catgggattc
     1441 agcaataaaa agagcgggta tccgatcccg taaagcgtat cagtcacgcc atacctatgc
     1501 gtgctgggct ttatctgccg gagcaaaccc gacatttatt gcatcacaga tggggcactc
     1561 cagcgccagc atggtctaca atgtttatgg tgcatggatg cctgagtgca gcgtgactca
     1621 agttgccatg ttgaataatg tccttaatgc ccgtgcccca gacgtgcccc aaagtgacca
     1681 ggaggatgaa ataaaattat atttttcaaa atgataaacc atatccttag acatgttgaa
     1741 acgatggcac gcgcagtcgc tgagggtgca agcaaagtcg atggcgcaga agttgtcgtt
     1801 aagcgtgtac cggaaaccat gccgccgcaa ttatttgaaa aagcaggcgg taaaacgcaa
     1861 actgcaccgg ttgcaacccc gcaagaactg gccgattacg acgccattat ttttggtaca
     1921 cctacccgct ttggcaacat gtccggtcaa atgcgtacct tcctcgacca gacgggcggc
     1981 ctgtgggctt ccggcgcact atacggaaaa ctggcgagcg tctttagttc caccggtact
     2041 ggcggcggtc aggaacaaac tattacttca acctggacga cccttgcgca tcacggcatg
     2101 gtaattgtcc ccattggcta cgcagcgcag gaattatttg acgttacaca ggttcgcggc
     2161 ggtacgccgt acggcgcaac caccatcgca ggcggtgacg gctcacgcca gccaagccag
     2221 gaagaactgt ctattgctcg ttatcaaggg gaatatgtcg caggtctggc agttaaactt
     2281 aacggctaat cttcaacagg aggatacgca tgccaactca agaagcgaaa gctcatcacg
     2341 tcggtgaatg ggcatctctg cgcaatacgt cgccggaaat agccgaggcc attttcgaag
     2401 tcgccgggta cgacgaaaaa atggcggaaa agatttggga agaaggtagc gatgaagtgt
     2461 tagtcaaagc ctttgctaag accgataaag attcgctttt ttggggcgaa cagaccatcg
     2521 aacgtaaaaa cgtttaacca gcgactcccc cgcttctcgc gggggagttt tctgttattt
     2581 caccgcttca ttcaacacgc tatcaaattt atccatcggg cagaaaccgt tggcatctat
     2641 cgggcaaccg cttaattcca gcgtcacacg ctgagcaggt gcctgcaggg ttaacgcatc
     2701 ggcattacgt aactgttccg cactctgata cacatattca attttcatca aatcgcgatt
     2761 ggctttgctg tcatgccaac gctggaaaac gattttgccg ccaatcggcg tgcgttcgtt
     2821 ctggtcatgc aactgatacg gtttgaaatc cagcgccgtt aacagagagg caatgttgga
     2881 gtcgtgccca accaacactg taattttcgg tgcgctggtg cgatcggtga ccagagcttt
     2941 gtcgatataa ctgaccagcg gtttcgcaac attgcgcgcc acttccggtg aggtaaacag
     3001 gctgtcctgg tagccgtttt taagcttcga caacaccttc cactgcttgt cagatttgat
     3061 ttctccccag gccacctgat ccatcggaaa accttcgtaa tattgcaaag taaacgcatc
     3121 taccagcgag ttgccgactt tcagctgccc ggaaacacct ggttcttgtt gatacttggc
     3181 gctaaaggta tttttgccat ccaccagcga acactgttgt ttctctttac aggcagggga
     3241 atctttatag ttaacgattt tttccagtag ctggtagctg tcggtaagct ggagtttgct
     3301 gagctctttc tccattgccg ccaccgcctg ttcactgaat gcggcggaat catcggtgat
     3361 caccgggtta aaggttgggt ccatagtgcc cattttttcc tggtgatgca caggaatatc
     3421 acaccccggg aatgcgccgg taataaagaa ctgtgcggtc gcaacggtac gttgcagact
     3481 attggcatag gcataaacgg tgtccggcgg cgggcattcc cccgatttca ccatcccctg
     3541 ctgtgccagc cattcacgca tgtaatggcc catatacact tcgagcacgc cacctttggt
     3601 ggtgagttgc ccaccgggga cgtcccattc gggccattta ttcggcgtag actgctccag
     3661 cacactgcca ttgttcgcca gcggcgcacg taagttatgg cggctcatca tgagcacttg
     3721 ctgtagctga tagccttccg gtacggtttg tgcctgagca tttgaagcga gtaaaactat
     3781 ccctgccaca gttgcggcga ttagcgtttt gttcattcct ggcacctctt ttgttatcaa
     3841 tcactaaaga gtgtgacaga aatatgacat ttttccggga acaggttcgc aaaatggctt
     3901 gttgaatgcc tgtttttcta tatacatctt taaaaacagg caaggtttga cgcaaactat
     3961 gcctgattag ctccctgcct cgcgtacgcc gctgatgatt acacctgtgg ctaaacagaa
     4021 tatctgcggc gttaccaata cagagacaat atttaccaat aaaaaagtgt gagactaata
     4081 acaatggttt ttagcatatg cctttgccat taaattgcat aacgatttaa atagtcgagt
     4141 tgcgcattca tttccttatt ttttaactta taagcctgag caaaaagttc aggagggagg
     4201 taaacatcat aatgattgcc agcgtttatt aaataaccat actcaaggtc gtcatctgtt
     4261 gaaaaggatt cattccccaa ggagattgat gccatattac tattaacaat aaataatgag
     4321 caaatgctat ctgaaaga
//
LOCUS       DAZFVO020000262         4269 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFVO020000262 DAZFVO020000000
VERSION     DAZFVO020000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.264"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(156..1121)
                     /locus_tag="ACHKNB_RS24370"
                     /old_locus_tag="pgaptmp_004874"
     CDS             complement(156..1121)
                     /locus_tag="ACHKNB_RS24370"
                     /old_locus_tag="pgaptmp_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGU4198231.1"
                     /translation="MLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLSVKDHQHID
                     SAIPGRYTVQAPYKTGAGIGVLKLITEHNRAHAVFSCHEHYYAQIMVGRTGPTSVGPG
                     SVVSGNANPVRLTTSVIGVPCGEFSKKLTTGAVTMTTLPTLSQPEIAIVDGQAVTSSL
                     AVADFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNGGTRKLPCYQITRDGF
                     AFLAMGFTGKRAARFKEAYINAFNQMEKQLSKPSTLSDAAHNASVLYSHLSVIHKVWL
                     QQLYPMLAKVESPLAISLYDHINDAAAISSLINMTLNPSEVRGRK"
     gene            complement(1075..1170)
                     /locus_tag="ACHKNB_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /pseudo
     CDS             complement(1075..1170)
                     /locus_tag="ACHKNB_RS24375"
                     /old_locus_tag="pgaptmp_004875"
                     /inference="COORDINATES: protein motif:HMM:NF025339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(1316..1867)
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS24380"
                     /old_locus_tag="pgaptmp_004876"
     CDS             complement(1316..1867)
                     /gene="ymfL"
                     /locus_tag="ACHKNB_RS24380"
                     /old_locus_tag="pgaptmp_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HGU4198232.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPDIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAMNDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(1860..2120)
                     /locus_tag="ACHKNB_RS24385"
                     /old_locus_tag="pgaptmp_004877"
     CDS             complement(1860..2120)
                     /locus_tag="ACHKNB_RS24385"
                     /old_locus_tag="pgaptmp_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4198233.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNRNELKPQEKEVSRG"
     gene            2218..2910
                     /locus_tag="ACHKNB_RS24390"
                     /old_locus_tag="pgaptmp_004878"
     CDS             2218..2910
                     /locus_tag="ACHKNB_RS24390"
                     /old_locus_tag="pgaptmp_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGU4198234.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSTGEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYITDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            3690..4052
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS24395"
                     /old_locus_tag="pgaptmp_004879"
     CDS             3690..4052
                     /gene="yfdP"
                     /locus_tag="ACHKNB_RS24395"
                     /old_locus_tag="pgaptmp_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HGU4198235.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            4118..>4269
                     /locus_tag="ACHKNB_RS24400"
                     /old_locus_tag="pgaptmp_004880"
     CDS             4118..>4269
                     /locus_tag="ACHKNB_RS24400"
                     /old_locus_tag="pgaptmp_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4198236.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNL"
BASE COUNT         1170 a         1027 c         1029 g         1043 t
ORIGIN      
        1 gcggtaatta cggcccagcg gttcacaaat caccttctga cattcacggt caaccaggct
       61 aacacgtaga acatgccctg caggcgtggt gtaccactga cccgggcgag gacaacggaa
      121 agtctgatcg gtaaaccgtt tgaaaatatt ccggatcatt tgcgccccct tacctctgaa
      181 gggttcagtg tcatattgat taggctggaa atcgcagcag catcgttgat atggtcatac
      241 aggcttatag ccagcggaga ttccactttg gccagcatgg gatagagttg ttgcagccag
      301 accttgtgga ttaccgacag gtgggagtaa agcacgctgg cgttatgtgc ggcatcgctc
      361 agcgtggatg gctttgaaag ctgcttttcc atctggttaa aggcgttgat gtacgcctct
      421 ttaaaccggg cggcgcgttt gccagtgaag cccattgcca ggaacgcgaa gccgtcgcgg
      481 gttatttgat agcaaggaag tttgcgagta ccgccgttgg gctggcgtac caaaattgat
      541 gtctccgcaa aattgcgggc acaaaactct ggagaacaat ccaaaatgcg gatctttttc
      601 agaacatcgt catgacgttt agagaaaaag tcagcaacag ccaaagaaga tgtaacagcc
      661 tgaccatcaa cgatggcaat ttcaggttga gagagggttg ggagagtagt catagtgaca
      721 gccccggtag tcagtttttt agaaaactca ccacatggga cgccaatcac agaggtggtg
      781 agacgtacag ggttggcgtt accggagact accgaacccg gcccgaccga agtcggccct
      841 gtacgcccca ccataatttg ggcgtagtaa tgctcatgac acgaaaaaac cgcatgagcg
      901 cggttatgct cagtaatcaa tttcaggacg ccaatcccgg cacccgtttt ataaggtgcc
      961 tgaacagtgt aacgtcccgg aattgcagaa tcaatatgct ggtggtcctt cacactcaac
     1021 aaaatcacgc ctgaatttcc acaaaggact aaagcactca tgcgggtagt ctttgcgaag
     1081 atagataacg cgctgtgttt ctggctccca acgaataaca tgaacataaa gtcctcttcc
     1141 gtcacgaaac cagcggttaa gttcctgcac aactcgcccc ccacagtcag gtaaagttct
     1201 ctgtggttac ttacagccag gtgatttggt aatctgcatt catgccgtaa caacaggtgt
     1261 gcagcgacac tgaccaccag ctgttgcgac aaacggttat ttgccgttaa actgttcatg
     1321 cgttagtttc tccacagaca caaaacgcca cgacgcccgg agctgcacac tcgcgggcgt
     1381 cactcttttc tggagcgcag aaaattttgt agaccagtgc cgcatgctcc tggagcttcg
     1441 aaattgacag atacaactca tcattcattg ctgtctgctc gtgtggctcc actaccccat
     1501 cttcgattgc cgaacgaatc tgctttgagt aactcccgat ctgttcgatg acttccagca
     1561 ggcgctggtt tatatcggcg ttctctactt cctcaatgtc aggaagcgat acaaacaccc
     1621 caccagcaga ctgtgcgaca gcatccgcaa tgtagtgagt gccagccgcg cgctgtaaaa
     1681 tcattgccca tcccagcggg aaaatctgat cgccatcggc acgaaggcga ttgaataaag
     1741 cgttctctgt tacatccagc cactcagctg cttcagcgta accacccggc aacgccgcga
     1801 tagtttttct gaccgctttc acgtaccact caggctgttt ttccactttc cagtgatgct
     1861 tacccacggc ttacctcctt ttcctgtggt tttaactcat tccggttttg actagattga
     1921 aagcgagcag gatagagaat ctgcatttcg ctgatttctc ccttaaaaaa attggccaga
     1981 cgctctgcca gatcgataga tggaatttgt tccagtctct caatacgact cagcgtcgct
     2041 ggattaacct gaacacccgc agcaacatgc tgcaaagtaa atccgtgcgc cttacgcaca
     2101 tttcgtaatg gtgattgcat ataacctcca catattgcgt gataagcata ttatttcacg
     2161 caaatatttt gcgcaagttg atttgcttaa cgcgcaataa agaaatgtaa taaacgcatg
     2221 aacataggaa atcgagtcag acaacttcgc caggcgaaga acatgaaaat cgccgatctc
     2281 gctgaagcaa taggagtgga tgcggcgaat atctcacgcc tggaaacagg taagcagaaa
     2341 caattcactg aacaagccct gagtaatatt gccaggagct taggtgttga tattgcagat
     2401 ctctttacct cagacttcaa aagtaatact gtatgtaaaa acagtactgg tgaggatgtt
     2461 gcgcaggtga aggatgtatt ccgtattgaa atgctggatg tcagtgccag tgcgggaaat
     2521 ggccttatcc agggcggtga tgtcattgat gtgattcatg ccattgaata cataactgat
     2581 aatgctgtat cgatgtttgg aggacgacca gccaatcaca ttaaagttat caacgttcgt
     2641 ggggacagta tgtgtccaac cattgagcca ggagatctca tcttcgttga tatcagtatc
     2701 aatcagtttg atggggatgg tatctatgtg tttggttttg atgataaaat ttatgtcaaa
     2761 cgactgcaaa tgatacctga caaactactg gtgatttctg ataaccagat ttaccgtgaa
     2821 tggggaatta ccagcgaaaa cgaacaccgg tttatggtct ttgggaaggt cttaatcagt
     2881 cagtcacaaa cccttaagcg acacaattaa ccccgacctc ctcatcaatt agccaccaga
     2941 aggtggcttt tcatcaccca tcatattgcg catctcgcaa caaaaacact tgcataatgc
     3001 gcaatttcat tttatctttc tttccagacc aacaaacaag gtcctaacaa aatttggttg
     3061 taacacggcg tatggcacat gcgtcgttag cggtctgggg acgttaaagg ggacaatcca
     3121 ctccttgctc gggcaaacaa accaggtagc cggaatgtgc aagtcaatga ggatgctgat
     3181 aagacgccta accagcgtgg cgattcggtt tgacgcctgg gaagagacca ggacgcaacg
     3241 atgagagcat tgacgagcaa ggcataagtg ctggttcaat tccagacagt cccattcaga
     3301 tgggagggtt gggcagggaa aaggtccgtt cgattcggac accggcaatg ctctcagcgt
     3361 tgtggtgaat gcgcaggctg atgcgcgaaa gacattgcag ctattgcgga aaagagctgt
     3421 tcggcggggc aatcaaacgc ccgtgagagt ctgaaataac cgcaagccgg agatcagcac
     3481 cggtcaccac aacagccact gctttggcag taccagtttg tacacttgct tccggctggt
     3541 accgctcttt ttacaaaaca gagaagagca tcaccggacg acgggctcat aacccaatcc
     3601 atccgggcgg ctgccaccgc aggtgttctt ctctgttttg tggagaaacc aaccgacctt
     3661 gcagggtcga tatgatgagg agcaacaaaa tggctagcga acgcagtact gatgtgcagg
     3721 catttatcgg ggagctggac ggcggcgtat ttgaaacaaa aatcggcgca gttctcagtg
     3781 aagtcgcttc cggtgtgatg aacacgaaaa ccaaaggtaa ggtctcgctc aacctggaaa
     3841 tcgaaccgtt tgatgagaac cgtgtgaaaa tcaaacacaa actctcatat gttcgcccga
     3901 ctaaccgcgg gaaaatttcc gaagaagaca ccaccgaaac gccgatgtat gtcaatcgcg
     3961 gtggtcgcct gactattctg caggaagacc agggacaatt actgactctt gccggtgagc
     4021 ctgacggaaa actccgcgca gcaggtcatt aatatcgttc ttaattaact gattatttat
     4081 ctcatcactg aatatcttaa tatagtgagg acttattatg tctcagaact tagacgcaac
     4141 cgcaattaat caaatccatg cccttatttc tgctcagggt gttaatgaaa ttatcagtaa
     4201 gattggtgcc gatgctgtgg cattgcctga gaatttccgc attcatgatc tggaaaaatt
     4261 taatttaaa
//
LOCUS       DAZFVO020000263         4191 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFVO020000263 DAZFVO020000000
VERSION     DAZFVO020000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.265"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..699
                     /locus_tag="ACHKNB_RS24405"
                     /old_locus_tag="pgaptmp_004881"
     CDS             <1..699
                     /locus_tag="ACHKNB_RS24405"
                     /old_locus_tag="pgaptmp_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198237.1"
                     /translation="VLWLAHTGTFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAV
                     CATCAPSLGADPARPLQTLALPSRMAPAPADRLTREERNSLLWEGIATVSVAAGDVLQ
                     IERQVTMYRQNVYGESDPSYLDVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRA
                     GQNIVTPEIMKLQLISLGEEWVELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLV
                     NQFRFLAAQIRFIL"
     gene            708..1073
                     /locus_tag="ACHKNB_RS24410"
                     /old_locus_tag="pgaptmp_004882"
     CDS             708..1073
                     /locus_tag="ACHKNB_RS24410"
                     /old_locus_tag="pgaptmp_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4198238.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISAKFASATSTRVQ"
     gene            1088..1564
                     /locus_tag="ACHKNB_RS24415"
                     /old_locus_tag="pgaptmp_004883"
     CDS             1088..1564
                     /locus_tag="ACHKNB_RS24415"
                     /old_locus_tag="pgaptmp_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4198239.1"
                     /translation="MTTRKKKNAVSDEAVIEAIRDALEDGDPRTAGLAEQLAKGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETDAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGDIQGPLSQRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRAGAVSGAD"
     gene            1692..3746
                     /locus_tag="ACHKNB_RS24420"
                     /old_locus_tag="pgaptmp_004884"
     CDS             1692..3746
                     /locus_tag="ACHKNB_RS24420"
                     /old_locus_tag="pgaptmp_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4198240.1"
                     /translation="MAKNLKASLIVDLLGNVSAKSRQWSQELGAFSRVGRTGLGGIGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTLGRLSNLYGMLAGG
                     AAVYGFNKAFIRPATEMENYILRLNAINHGDTAKTEAVKAWAVQNAKETTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAS
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDGFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAAAGFYRAIQKVRETLQALRDAGYGDALDRIAQGAQTAAKYLLYMYL
                     ATRALKAVRAIGLGALRPGAALLGYGLSTAAVLTSPFRKPQTTVPGTQPGRGQRFMNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVPTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLSAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDNSASAADKAGAMGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     EAWQKLRGSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNYG
                     LY"
     gene            3733..>4191
                     /locus_tag="ACHKNB_RS24425"
                     /old_locus_tag="pgaptmp_004885"
     CDS             3733..>4191
                     /locus_tag="ACHKNB_RS24425"
                     /old_locus_tag="pgaptmp_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HGU4198241.1"
                     /translation="MASTDTPGRGSFRGVPFLIYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTALVIGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAVLEQKSGSAMNQLFNTLSD"
BASE COUNT          912 a         1100 c         1326 g          853 t
ORIGIN      
        1 gtgctgtggc tggcacatac cggcacgttt ggtgaggtgc aggcattcgg ggcaaaacgc
       61 aatgattttc tggcgacctg cacatccatt cccaaagccc ctgaaccgga ttacctctgg
      121 gcagcggcgg tctgcgccac ctgtgcaccc tctctgggag cagatcctgc gcgtccgctt
      181 cagacgctgg cgcttccgtc acgcatggcc ccggctccgg ccgatcgtct gacgcgtgaa
      241 gagcgtaaca gtctgctgtg ggaagggatt gccacggtca gtgtggctgc cggtgatgtg
      301 cttcagatcg aacgtcaggt gactatgtat cgccagaacg tgtacggcga aagtgatccc
      361 agctatctgg atgtggaaac catctacacc ctctcctggc tgcgttattc cctgcgtact
      421 tttatcacac aacgttttcc gcgccataaa ctggctgatg acgggacgcc cgttcgtgcc
      481 ggtcagaata tcgtgacacc ggagatcatg aagcttcagt taatttcgct gggtgaagag
      541 tgggtggaac tcgggctggt cgaaaatctg gacaccttta aaaagaatct gcttgtggag
      601 cgtaatgcgt cagatcgaaa ccgccttgac gtgttatgca cgccggatct ggttaaccag
      661 ttccgcttcc tggcagcaca gatccgtttc attttgtgag gtaaggcatg agcggaaaac
      721 agtatcaggg cacggccact atccgtgtga acggtcagga atacgagaca cttgaggggg
      781 ctacattttc tccgtcaggt tttgaacgtg aagtggtgaa aggcgcgaaa gtctatggtt
      841 accgccagaa accacgtgaa gcgacgctgg actgcaaatt tccggcaggc ggagaaggtt
      901 caccggccgc tgatgaaatc aatacctgga ctgcggtcac tatcgagttt gtggcggata
      961 ccggcgaagt ccacatgatg acgaaagcct ggagcagtga accggcctca cttgacggtg
     1021 gcggggagat ctccgcgaag ttcgccagtg ccaccagtac ccgtgtccag taatcaggaa
     1081 aaatgcaatg accacacgaa agaaaaaaaa cgcggtttct gacgaagccg ttattgaggc
     1141 tatccgtgat gcacttgagg acggcgatcc acgcaccgcc ggtctggctg aacaactggc
     1201 gaaaggctat gtggatctgc tcgacggtct gccgttcggg gaatcccgtg aataccgcgt
     1261 caccttccgc gaactgacgg cgaaagacag cattgatgcg gaaaccgatg ctgaacgtct
     1321 tatggaaacc cgaaatggcc cggttctggt ggcatcaccg gccctgcgcg gtattgcgct
     1381 gctgcgtcgc cagattgccg ctgttggtga cattcagggg ccgttgtcac aacgccagat
     1441 tgggcagtta agcgagcgcg atctctcccg tctgatggca gcggtcagcc tgctggatac
     1501 cgcgcttgcc ggaaagctgg cggctgaccg ggggcgagca ggcgcagtgt cgggcgcaga
     1561 ttgaagaagc ggcaatagtg ctggggatgg tgaccagaag cggcccggaa tgggcgctta
     1621 acctcccctt atcgcaactt taccggcact gccaacaaac ggaaaatatt ctcagaacga
     1681 agcagtaacc catggcaaaa aacctgaaag cctccctgat tgtcgattta ctcggcaatg
     1741 tttccgccaa atcccgccag tggtcacagg agctgggggc gttctcccga gtcggcagga
     1801 cggggctggg tggaattgga aatgcagccc gccgtgccgg tcaggagaca gagcttgtcg
     1861 gctcccgtat gcagcgtacg cttgccggtg tccgtggcag tatccgcacg gtaacgtcgg
     1921 attttgaccg tcttcagggc agtattaccg gcacgctggg acgtctcagc aacctttacg
     1981 ggatgctggc aggaggggct gcggtctatg gttttaataa agcctttatc cgcccggcca
     2041 cagagatgga gaactatatt ctgcgtctca atgccattaa tcatggcgat acggcaaaaa
     2101 cggaagcagt gaaagcctgg gcggtacaga acgcaaagga aaccacctgg ggactggcgg
     2161 gggttatgca ggaatacgcc tccagtcgcg ggttcggtat gagtgacagg gaagcccgcc
     2221 gctttattac catgcttcag gatcagggcg gctatcacgg ctggtcactg tcagatgcac
     2281 agggtgcgtc cctgcaactc aaacagatgt ttgcccgcca gagtatccag gctgcggacg
     2341 ccagtatcct gaccggatac gggataaacg tttaccagtt gctggccgat aaactgggtg
     2401 taaaccagaa gatcatccgt gagaaaggcg agaaaggaaa actgggaccg gacagcattc
     2461 ggctgttatt tcaggtgatg gccgaacagg caaaaggtgc ccagaaaaac gccatgaact
     2521 catggacagg gatgacatcc atgatgggcg acgtctggga cggatttgcc cgtgaagtaa
     2581 tggcaaaagg gccatttgac agcctgaaaa agagcctgaa aggcttcctg gattatgccg
     2641 atgcggcaca gaaaagcggc ttacaggaca agctggccac acaaacagcc tcagccctga
     2701 atcaggggtt tgagtatgcc agagacgcag cggcgggttt ttaccgggcc attcagaaag
     2761 tcagggaaac gcttcaggca ttgcgtgacg ccggttatgg cgatgcgctg gatcgcattg
     2821 ctcagggcgc acagaccgcc gcgaaatatc tcctgtatat gtatctcgcc acccgtgcgc
     2881 tgaaggctgt ccgcgccata ggtcttgggg cactgcgacc gggggcggca ttgttggggt
     2941 acgggttatc cacggccgct gtgctgacct cgccttttcg taaaccgcag accaccgttc
     3001 cgggcacgca gccaggacgc ggccagcgtt ttatgaactt tcttaccggg gtaaatcctg
     3061 ctgccgttca gcccgtactg gtcaccaact ggcctgcggg tggtctggca tcaggtggcg
     3121 gggatgtggt ggtcagcggt gacggcaaaa ccgtgcgcgg ccgtaaaaaa cgcggccccg
     3181 gacgtgggcg gggtgtcccg accgttgtga ccgcaggcga acaactggca gagagcgccg
     3241 gtaaacaggg cttcttcggt cgtatgatga gccgtgccgg tggtctgctt tcggctgccg
     3301 gaaaccgcat ggggctgggg cgttttgccg ggttgttccg tggtgccggt cggctgggcg
     3361 gtggtgccct gtgggccggt gccatggcgg ctcctgtact gctggacaac agcgccagtg
     3421 cagctgataa agccggtgcc atgggttccc ttgccggaag tatcgccggt ggcgcgctgg
     3481 gggctgccgc cggtccggtc ggggttgcca ttggctccac ggtgggcagt tatctcggtg
     3541 attatctggg gggatggctg accgaagcct ggcagaaatt acggggcagt gatgaaaatg
     3601 gcggacaggc caccgcgaaa accgccgcac gggtggagct tgtggcccct gaaggctggc
     3661 gggcacgcag tattgatgtg gatgataccg cacagcatgg ccttgatgtg aacgtctgga
     3721 acggagggaa ctatggcctc tactgacacg ccggggcgtg gctcctttcg tggtgtgccg
     3781 tttctgattt atcaggaaca acgggaacgg ggcggacgta acattgtacg ccgtgaatat
     3841 cctctgcggg aaagcggcgg cgcggatgat ttggggccaa aactcccgga gtttacattc
     3901 acggctcttg tgatcggcga tgatcttcag acgcagcgaa gccgcctgcg ggatgcgctg
     3961 cgggcacccg gtgccggtga actgatgcac ccggattatg gcaccttaaa cgtgctgata
     4021 aacagctttg aaagccgtta taacgccagc gaacagggaa cggttgagtt cacgatcaac
     4081 gtgatccccg ccagtgacga taccgcaccg gcggtggctg aagacacggc tgccgttctg
     4141 gagcagaaaa gcggttcagc aatgaatcag ttgtttaaca cgctttcaga c
//
LOCUS       DAZFVO020000264         4148 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFVO020000264 DAZFVO020000000
VERSION     DAZFVO020000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.266"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1480
                     /locus_tag="ACHKNB_RS24430"
                     /old_locus_tag="pgaptmp_004886"
     CDS             <1..1480
                     /locus_tag="ACHKNB_RS24430"
                     /old_locus_tag="pgaptmp_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4198242.1"
                     /translation="NVMLGGTLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNY
                     TGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGG
                     NSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVD
                     PEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGG
                     HERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVG
                     YKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGV
                     TASVEGGYTFEAGTFSGSEGTLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGN
                     VQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLG
                     EVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            1689..1955
                     /gene="hipB"
                     /locus_tag="ACHKNB_RS24435"
                     /old_locus_tag="pgaptmp_004887"
     CDS             1689..1955
                     /gene="hipB"
                     /locus_tag="ACHKNB_RS24435"
                     /old_locus_tag="pgaptmp_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HGU4198243.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            1955..3277
                     /gene="hipA"
                     /locus_tag="ACHKNB_RS24440"
                     /old_locus_tag="pgaptmp_004888"
     CDS             1955..3277
                     /gene="hipA"
                     /locus_tag="ACHKNB_RS24440"
                     /old_locus_tag="pgaptmp_004888"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HGU4198244.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            3584..3792
                     /locus_tag="ACHKNB_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /pseudo
     CDS             3584..3792
                     /locus_tag="ACHKNB_RS24445"
                     /old_locus_tag="pgaptmp_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1151 a          910 c         1081 g         1006 t
ORIGIN      
        1 gaacgttatg cttgggggga cactgcgtgt cgctaaaacc acaatcggcg gcaacctgga
       61 gaatggcggc acggttcaaa tgaatagcga aggggggaaa ccggggaatg tactgaccgt
      121 taacggcaac tataccggaa acaatggcct gatgacgttc aacgcgacgc tgggcggcga
      181 taattcgccc accgataaaa tgaacgtgaa aggcgatacc caagggaaca ctcgcgttcg
      241 ggttgataac attggcggcg tcggtgcaca aacggtcaac ggtattgaac tcattgaggt
      301 tggcggtaat tctgcaggta acttcgcgct gaccaccgga actgtcgaag ctggggctta
      361 cgtctacacg ctggctaaag ggaaggggaa tgacgagaaa aactggtatc tgaccagtaa
      421 atgggacggc gtaacgccac cggatacgcc cgaccccatc aataatcccc ctgttgtgga
      481 tccggaaggc ccatcagttt atcgcccgga ggccggaagc tatatcagca acattgccgc
      541 agccaactcg ctgtttagcc atcgtttaca cgaccgtctg ggtgagccgc agtatataga
      601 ttcactgcat tctcaggggt cggcaagcag tatgtggatg cgtcatgtcg gagggcacga
      661 acgttcaagg gccggtgacg gtcagctaaa tactcaggct aaccgctatg tattgcagct
      721 aggcggcgat ttggcgcagt ggagtagcaa cgcgcaggat cgctggcatc ttggcgtgat
      781 ggcaggttac gctaatcagc acagtaatac tcagagtaat cgtgtgggtt ataaatcgga
      841 tgggcgcatc agcggttaca gcgctgggct gtacgcgacc tggtatcaga acgatgcgaa
      901 taagaccggc gcttatgttg acagctgggc gctgtataac tggtttgata acagcgtcag
      961 ttccgataac cgttctgctg acgactatga ttctcgcggt gtgacggcct ctgttgaggg
     1021 tgggtatacc tttgaagcgg gaacatttag cggcagcgaa gggacgctga atacctggta
     1081 cgtccagcca caggcgcaaa tcacctggat gggtgtgaaa gattccgacc atacccggaa
     1141 agacggaacg cgcattgaaa cggaaggcga cggaaatgtg caaacgcgac ttggggtgaa
     1201 aacctacctg aacagccatc accagcgtga cgatggtaaa cagcgtgagt tccagcctta
     1261 cattgaagcg aattggatca acaacagcaa agtctacgct gtgaagatga atggtcaaac
     1321 cgtaggccgt gaaggtgcgc gtaatctcgg tgaagtacgt accggggttg aggcgaaagt
     1381 aaataacaac cttagcctgt gggggaatgt cggtgtgcaa ctaggtgata aaggctatag
     1441 cgatactcag ggcatgctgg gagtgaaata tagctggtaa accgtataag ccgcatgtcg
     1501 agatggcatg cggcttaata ttgccgactt caaaggcttt aaagcctccc ttttatccgc
     1561 gatcgcggat atcgcagcgt ttatcccgta cagcggataa gatgtgtttc cagattgact
     1621 ttatcctcac taaaggataa aactgataat atccccttaa gcggataaat ttgctgtgga
     1681 cgtatgacat gatgagcttt cagaagatct atagcccaac gcaattagcg aatgcaatga
     1741 aactggttcg ccagcaaaat ggctggacgc agagcgagct ggcgaaaaaa attggcatta
     1801 agcaggcgac aatttccaat ttcgaaaaca accctgacaa taccacgctc acgacatttt
     1861 ttaagatttt acagtcgctt gaactctcaa tgacactatg cgacacgaaa aatgcctcgc
     1921 cagaatcaac agaacagcaa gatctggagt ggtaatgcct aaacttgtca cttggatgaa
     1981 caaccagcgg gtaggcgagt taacgaagtt agccaacggc gcgcacacct ttaagtatgc
     2041 accggagtgg ttagccagcc gttatgccag accgttgtca ctttcgctgc cattgcagag
     2101 ggggaatatc acctcagatg ccgtatttaa cttcttcgat aacctgttac ccgatagccc
     2161 gattgtacgt gaccggatcg ttaaacgtta tcatgctaaa tccagacaac cgtttgattt
     2221 attgtcagaa atagggcgag acagcgttgg tgccgtaacg ttattacccg aaaatgaaac
     2281 cataacgcgc ccgataatgg catgggaaaa gcttactgaa gccaggcttg aagaagtatt
     2341 aacggcttat aaagcagata tccctctggg catgattaga gaagaaaatg actttcgcat
     2401 ctcggttgct ggcgcgcagg agaagacagc gctgcttaga ataggcaatg actggtgcat
     2461 tccgaaagga ataacgccga cgacgcacat cattaaatta ccgattggcg aaatcaggca
     2521 gcccaatgcg acgctcgatc tcagccaaag cgttgataat gagtattact gtctgctgct
     2581 ggcgaaagaa cttgggttga atgttccgga cgcagaaatc attaaagcgg gaaatgtgcg
     2641 cgcgttagcg gtcgaacgtt ttgacaggcg ttggaatgct gagcgaacgg ttttacttcg
     2701 cttgccacag gaggatatgt gtcagacatt cggtttacct tcatcggtga aatatgaatc
     2761 agatggaggc ccaggcatcg cgcggatcat ggcttttttg atggggtcca gcgaggcgct
     2821 gaaagatcgc tatgatttta tgaaattcca ggtcttccag tggttgattg gcgcaacgga
     2881 cggtcatgca aaaaacttct ccgtatttat tcaggctggc ggcagttatc gactcacgcc
     2941 attttacgac atcatttcag catttccggt ccttggcggt acgggaatac acatcagcga
     3001 tctcaaactg gcaatggggc ttaacgcatc caaaggcaaa aaaacggcaa tcgataaaat
     3061 ttatccgcga cattttttgg cgacagcaaa ggtgctgaga ttcccggaag tgcagatgca
     3121 tgaaatccta agtgactttg ccagaatgat tccggcagca ctggataacg tgaagacttc
     3181 attaccgaca gattttccgg agaacgtggt gacggcagtt gaaaccaatg tgttgaggtt
     3241 gcatggacgg ttaagccgag aatacggtag taagtgagat atggggtttg gtcgttgtta
     3301 atgaacaatc atgaccgcca acgactggat tcttccttgg tttatactgg tgcgcataat
     3361 gtatattatg ttaaatcatg tctgagtgat gcgaagtgct tttctggtag tcgttattcg
     3421 ttcagggttc aagaacttaa gcctctcctc tttcataatt ggcctgtgca tggagaaata
     3481 gcctggttat tccctgcccc atgctaagag cgcagtgtct gtcgcataga cactgcatac
     3541 gaaaacacca gagggtgctg ttacttattt aaggacggat caagcgggaa tgagtccact
     3601 ccggtatcag aaatggctac gtctcaatga agtcaggcga ccgatgctga atgaacatta
     3661 cgatgtcacc acagcagcct atgctgtcgg ttacgaaagc tatcccattt cagtcgggaa
     3721 tattcgcgga tgtttggaga gtcacccaag agagatatta ccaggttaag aaagtctgtc
     3781 ggtcagcttt aataaaccct gcacttatct gtttagagca attcggtgtt agtttcagca
     3841 agcaaacatt aaccatagct aatgatttat agccatatta accattggta gcccttgacc
     3901 tttcctgaca cttcccttgt tcgcaataat attctgttta gtcacggttt ataaataaca
     3961 tgcataatga aatcgtcatg ttatttagcg acataaaatg tttttgcaaa tactctcttc
     4021 aacaagtgca gcaataaact tgttgtgatt aggtgtcctt gtgatttaaa gatattatcg
     4081 ttcttcgtga aatgtaataa ccctggaaac tatattctca gggcaacaac caatcaagaa
     4141 tgattctt
//
LOCUS       DAZFVO020000265         4122 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFVO020000265 DAZFVO020000000
VERSION     DAZFVO020000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.267"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..3086
                     /gene="evgS"
                     /locus_tag="ACHKNB_RS24450"
                     /old_locus_tag="pgaptmp_004890"
     CDS             <1..3086
                     /gene="evgS"
                     /locus_tag="ACHKNB_RS24450"
                     /old_locus_tag="pgaptmp_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HGU4198245.1"
                     /translation="ANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNI
                     ITSSMISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVS
                     QNWLDTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGD
                     ILNIITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITT
                     PYVFVMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKE
                     GELDALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALN
                     AIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVR
                     RRKVIQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLP
                     LENSDSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDN
                     AVYICGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLEL
                     LSGSGLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQN
                     TCHSFGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKIT
                     TSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKEL
                     IKNMQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHP
                     TNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLRE
                     QNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYR
                     HLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGA
                     ANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3142..>4122)
                     /gene="yfdE"
                     /locus_tag="ACHKNB_RS24455"
                     /old_locus_tag="pgaptmp_004891"
     CDS             complement(3142..>4122)
                     /gene="yfdE"
                     /locus_tag="ACHKNB_RS24455"
                     /old_locus_tag="pgaptmp_004891"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HGU4198246.1"
                     /translation="YVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAE
                     NFRPGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMET
                     GYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHG
                     LMAYIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVN
                     DPRFSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQ
                     TQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1250 a          911 c          820 g         1141 t
ORIGIN      
        1 tagcaaatta ccccccagac gaggtaattc atcaatcatt tccaaaagca acaattatct
       61 cttttacaaa tttatatcag gcattagcat ccgtctcagc tgggcacaat gattacttta
      121 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
      181 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatctg
      241 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
      301 aagtatcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
      361 tcactgaaca tgaaaaacag tggattaagc agcatcccaa tttaaaggtg ctggaaaatc
      421 cttactcgcc cccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
      481 acattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
      541 acaatatcca tgctggaaca cagcttagcc ccggaggatg ggatataata cctggcgcta
      601 tttatagtga agatcgagaa aataatgttt tatttgctga agccttcata acaacgcctt
      661 acgtttttgt catgcaaaaa gcgcctgaca gtgaacaaac attaaaaaaa ggaatgaaag
      721 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
      781 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
      841 atgctctggt cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg
      901 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
      961 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
     1021 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
     1081 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
     1141 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
     1201 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
     1261 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
     1321 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag
     1381 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
     1441 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
     1501 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
     1561 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
     1621 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
     1681 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
     1741 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
     1801 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
     1861 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tccatcgcat
     1921 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgatcct caggcgttta
     1981 agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa
     2041 aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta
     2101 tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc
     2161 aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat
     2221 taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat
     2281 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
     2341 aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
     2401 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
     2461 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
     2521 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
     2581 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
     2641 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
     2701 taagtcagtt acaccaggtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
     2761 ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc
     2821 agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca
     2881 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
     2941 agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg
     3001 aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg
     3061 ctgttttctg tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct
     3121 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
     3181 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
     3241 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
     3301 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
     3361 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
     3421 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
     3481 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
     3541 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
     3601 cagacgttgt ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact
     3661 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
     3721 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
     3781 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
     3841 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
     3901 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
     3961 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
     4021 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
     4081 atgattaata aaactgtaat agagtgactg tccatccaca ta
//
LOCUS       DAZFVO020000266         4060 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFVO020000266 DAZFVO020000000
VERSION     DAZFVO020000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.268"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            32..1036
                     /gene="msrP"
                     /locus_tag="ACHKNB_RS24460"
                     /old_locus_tag="pgaptmp_004892"
     CDS             32..1036
                     /gene="msrP"
                     /locus_tag="ACHKNB_RS24460"
                     /old_locus_tag="pgaptmp_004892"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HGU4198247.1"
                     /translation="MKKNQFLKESDVTAESIFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1037..1672
                     /gene="msrQ"
                     /locus_tag="ACHKNB_RS24465"
                     /old_locus_tag="pgaptmp_004893"
     CDS             1037..1672
                     /gene="msrQ"
                     /locus_tag="ACHKNB_RS24465"
                     /old_locus_tag="pgaptmp_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HGU4198248.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            1929..2579
                     /gene="zinT"
                     /locus_tag="ACHKNB_RS24470"
                     /old_locus_tag="pgaptmp_004894"
     CDS             1929..2579
                     /gene="zinT"
                     /locus_tag="ACHKNB_RS24470"
                     /old_locus_tag="pgaptmp_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HGU4198249.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            2922..3452
                     /locus_tag="ACHKNB_RS24475"
                     /old_locus_tag="pgaptmp_004895"
     CDS             2922..3452
                     /locus_tag="ACHKNB_RS24475"
                     /old_locus_tag="pgaptmp_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HGU4198250.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT         1142 a          922 c          885 g         1111 t
ORIGIN      
        1 gctggctaac gacaaaaaag tgtgatggct tatgaaaaag aatcaatttt taaaagaatc
       61 agatgttacg gccgagtcga tattctttat gaagcgtcga caggtgttaa aagcactggg
      121 catcagcgca gctgcacttt ctttgcctca cgctgcgcat gccgatctgc ttagctggtt
      181 taaagggaac gatcgcccgc ccgcccccgc cggaaaaccg ctggagttca gcaagcctgc
      241 cgcctggcaa aataacctgc cactgacgcc agtagataaa gtctccggtt ataacaactt
      301 ctatgaattc gggctggata aagccgatcc cgccgctaat gctggtagcc tgaaaaccga
      361 tccatggaca ctgaaaatca gcggcgaagt ggcaaaacca ttgaccctcg atcacgatga
      421 tttaacccgt cgcttcccgc tggaagagcg tatttatcgt atgcgctgcg tggaggcatg
      481 gtcgatggtg gtgccgtgga ttggttttcc gctgcacaaa ttgctggcgc ttgccgaacc
      541 caccagcaat gcgaagtatg tcgctttcga aacaatttat gcaccggaac agatgcctgg
      601 ccagcaggac cgctttatcg gcggcgggct gaaatatcct tatgtcgaag gattgcgtct
      661 cgacgaagca atgcatccgc tcacactgat gaccgtgggt gtttatggca aggcgttacc
      721 gccacaaaat ggcgcgccgg tacgactgat tgtgccgtgg aaatatggct ttaaagggat
      781 taaatcgata gtcagtatta agctgacccg cgagcgtccg ccaaccacct ggaatctggc
      841 agcgcctgac gaatacggtt tttacgccaa cgttaatccg catgttgatc acccgcgctg
      901 gtcacaggct accgaacgat ttattggttc aggcggcatc ctcgatgttc agcgccagcc
      961 aacgctactg tttaatggtt acgccgacca ggtggcatcg ctgtatcgtg gcctggattt
     1021 gcgggagaat ttctgaatgc gtctgacagc aaaacaggtg acatggctga aagttagcct
     1081 gcatcttgcc ggattgttgc cgtttctctg gctggtctgg gcgatcaatc acggtggact
     1141 gggtgccgat ccggtgaaag atattcagca ttttaccggt cgcactgcac tgaaattttt
     1201 gctggcgacc ttgttaatca cacctctggc acgctacgca aaacagccgt tattgatacg
     1261 cactcgccgc ctgttaggct tatggtgctt tgcctgggcg acactgcatt taaccagtta
     1321 cgcattgctg gagctgggcg tgaataatct ggcgttatta ggcaaggagt taattacccg
     1381 gccttattta acgttaggaa ttatcagctg ggttattttg cttgctttag cgtttacttc
     1441 aacccaggcg atgcagcgaa aactcggcaa gcactggcaa cagttgcata atttcgtcta
     1501 tcttgtcgcg atcctggccc caatacatta tctgtggtca gtgaagatta tttcaccgca
     1561 gccgctcatc tacgctgggc tggctgtact gcttttagcc ttacggtata agaagttgcg
     1621 ttcccttttt aaccggttac gcaagcaggt tcacaacaaa ttatctgtat aacttcatat
     1681 taccgacaaa gtacgcgtta tccctgccgg atgcggcgcg agcgccctat ccggcctacg
     1741 cagatcacat caggttatgt ttcgtttgtc atcaacctca aattatcaac ataattcatt
     1801 ttatttcaat aagatagata agtatacctg agaaaactat gctctcatct cctaagagtt
     1861 attgcatttt gtcatatgtt acaatataac attacacatc atatacaatg actctggagg
     1921 aaactgtttt ggcgattcgt cttcacaaac tggctgttgc tttaggtgtc tttattgtta
     1981 gcgctcctgc cttttcgcat ggtcatcact cacacggcaa acccttaaca gaggtcgaac
     2041 aaaaagctgc taatggtgtt tttgatgatg ccaatgtaca aaaccgaacg ctcagtgact
     2101 gggatggagt ctggcaatcc gtttatcctt tactgcaaag tggcaaactt gaccccgtct
     2161 ttcagaagaa agcggatgca gataaaacta aaacatttgc tgaaattaaa gattattatc
     2221 acaaaggtta tgcaacagat atcgagatga ttggcattga ggacggcatt gttgaattcc
     2281 atagaaataa tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct
     2341 ataaatcagg caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag
     2401 cccctaaata tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc
     2461 acatttttat gggtaatgat tcacagcaat ctttattaaa tgaaatggaa aactggccaa
     2521 cgtattatcc atatcagttg agtagcgaag aagtggtcga ggaaatgatg tctcattgag
     2581 tattctcatg ataacgcctc gatgccgctt tagtaagtca tcataactgc cactggtcca
     2641 tccacaaacg ccactgaacg caagctagct acagacacgc tcatcactat gacgtgtctg
     2701 tatattaata agctaacccg cattgagtta accaataacg gattccatac acaatacggc
     2761 caacagagaa agatacctgt gctcacgcca ttgcttatat tggctggtta caatgtgcac
     2821 tatcaatttt ttaaataaca aattcttaaa accacatctg tataaggatt ttttaaggtt
     2881 cgtgggatag cttgactgta aaaattacag gagctacaaa aatgaaccga ttctcaaaaa
     2941 ctcaaattta tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg
     3001 aactccgtgg ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt
     3061 acaatgcggg tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt
     3121 atagtgatcc ttctattgta ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa
     3181 tgcacatcat gctttatata accttccttg cacttcctct gctggggatt gctttaatgg
     3241 cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa
     3301 acagcgaaat taaagcactg ataaaaaata ttcacgaaac ctgggcaaat ataggttact
     3361 ttttaatcgc agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata
     3421 ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc cctgacaagg aaatgtgaac
     3481 catagacatc aacgcgtcat aaatgtttac gcaagtgcac aatgcaccct ttccactatc
     3541 aacaaggagg agcagaaact gtgtaaatcg tacaatggta agacttatca aaaatacagg
     3601 ttaattttgg tatttaaacc ttttttactt gttagtatga tacaaaggct ttcaaaaaag
     3661 ctgggaaatt caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg
     3721 ccgcgttcct tttttgactc aattattgac cccttctcta cgcaacttca gttcccacca
     3781 ccaactttgc ggcagtttca taggatgaat gtctaaaaga ataatggtga gcgataagaa
     3841 aacgactgaa taactgcaca ttttcgctcg aaagcttcct gtcagatcca tagcgaatca
     3901 agtgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt
     3961 ctacgattgt tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa
     4021 gtggcggaga gagggggatt tgaacccccg gtagagttgc
//
LOCUS       DAZFVO020000267         3987 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFVO020000267 DAZFVO020000000
VERSION     DAZFVO020000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.269"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..1226)
                     /locus_tag="ACHKNB_RS24480"
                     /old_locus_tag="pgaptmp_004896"
     CDS             complement(<1..1226)
                     /locus_tag="ACHKNB_RS24480"
                     /old_locus_tag="pgaptmp_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HGU4198251.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPG
                     ETGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPAGEKGDTGPQGATGAQGPAGATG
                     AVGPKGEPGPKGERGETGPQGPKGDKGDPGGPPGPKGDTGPRGEAGPPGPQGPAGQPG
                     PKGDKGEPGATGPAGPAGPRGETGPAGPAGPAGSVASVPDASTSQKGVVQLSSDTNSD
                     DETKAATPKAVKAVMAEVQAAKTKAEEAATRAAVPGPKGDRGEPGAPGAVGPAGPRGP
                     AGAAGPKGDAGPAGPAGKDGTAGAEGKAGAQGPKGDKGDPGEAGPAGPAGPKGERGEP
                     GPMGPPGKDGATSLVSG"
     gene            complement(1411..>3987)
                     /locus_tag="ACHKNB_RS24485"
                     /old_locus_tag="pgaptmp_004897"
     CDS             complement(1411..>3987)
                     /locus_tag="ACHKNB_RS24485"
                     /old_locus_tag="pgaptmp_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HGU4198252.1"
                     /translation="TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQD
                     RPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDP
                     DAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDI
                     IEICDNDYAGTLTGGRVLSIDAATRTLTLDREVTLPGTGASTVNLINGSGKPVSVDIT
                     AHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVAIRENTDGTFAITAVQHVPE
                     KEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLALAKWDTPRVVKGVRFS
                     LRLTSGSGENSRLLTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRIN
                     APAAPAGVELTPGYFQITAVPRLAVYDPTVQFEFWFSEAKIADAAQVETSARYLGTGS
                     QWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAAGYLELFREKIGKTHLA
                     EALWAEIDNSQLKDEMAEMQTTITETRNEITQTVSKTLEDQNATIQQIQRVQTDTNND
                     LNSMWAVKLQQMQDGRRYIAGIGAGIENTPDGMQSQILLAADRVAFINPENGDTTPAL
                     VTQGGQTFINEALIKVLTAPTITSGGNPPAFSLTPDGRLTARNADISGNISASSGTLN
                     NVTIAEDCTINGTMSADRIIGDIVKGYTVDMGQGVMTQNQLTFQKMILLKDNMPFDRK
                     LMLMAYSFSRGEFIMKINDKVVVHARSGKVKTGEREVQISKNASRYEPVYSDVGGSAS
                     GSWSIPAGQGDVRVEITFRYEADVLFYMGGSILHDKSKIRIHDPVLFYLKSYTDDFDT
                     AS"
BASE COUNT          693 a         1306 c         1031 g          957 t
ORIGIN      
        1 ccactaacca gcgaagttgc gccatctttc cccggaggcc ccatcggtcc cggttcaccg
       61 cgctcacctt tgggacctgc cggacctgct ggacctgctt ctcccggatc gcctttgtca
      121 ccttttggcc cctgtgcgcc tgccttacct tccgctccgg cggtaccatc ctttcctgcg
      181 gggcctgccg ggcctgcatc acctttggga ccagctgcac cagccggtcc ccgtgggcct
      241 gcgggcccca ctgcaccagg tgcgccaggt tcccccctgt cccccttcgg tccggggact
      301 gctgcccggg ttgctgcctc ttctgccttc gtttttgctg cctgcacttc tgccattacc
      361 gctttcacag cctttggggt tgccgctttt gtttcatcat cgctgttggt atcgctgctc
      421 agctgtacaa cacctttctg tgatgtgctg gcatccggaa cactggcgac actgcctgcg
      481 ggacccgcag gacctgcggg gccggtttct ccccgagggc ctgcgggacc tgccggacct
      541 gttgcgccgg gttcaccttt atcccctttc gggccgggtt gccctgccgg accctgtggt
      601 ccgggtggtc cggcctctcc acgtgggccg gtgtcacctt tcggccccgg cggtccgccc
      661 gggtcacctt tatcgccctt cggtccctgt ggacctgttt ctccccgttc tccctttggc
      721 cccggctcac cttttggtcc gaccgccccc gttgccccgg ctggtccctg tgcccctgtg
      781 gcaccctgtg gacctgtgtc ccccttctcg cccgccggtc cccgggcatt ctctgcccgt
      841 ttcttcgctt cctctgcact cgccgccgac gcttcagcac gcttcaggat ttccgctgcc
      901 accgcttcca gctctgcaag cgctttcggg taatactgcg cctcatcctg ttccatcaga
      961 aatttattca gcgttcccgg ctcagaatcc gccttcacca gaatgtcacc cacatacgac
     1021 ggggcgtacc cttccgtgtt cagcgtcacc cggtaccacc ccggctccac atcaaaactg
     1081 taactgccgg tttcccccgg ctgcccctgc gccaccgtgg tgactatcac tgtctctgtg
     1141 gtgcgtcgcg ctttcagctc tatggtgcat cccggcaccg gcttcccggt gccgtccttc
     1201 agcacgcccg atattctgac tgtcatagat ttctcccata aaaaaaaccg cagtaccggt
     1261 tacccggtct gcgatcaggg gtataaattg tgactggctg gtgttaaagg gtgccgtcag
     1321 tcaaaggcac ccgtggataa tgtacgatgt ccggcttttg tcgttttccg gcaatcacga
     1381 acagatggat gaaacctcac tatccacctg tcacgatgcg gtatcaaaat cgtctgtata
     1441 ggatttcagg taaaacagaa ccgggtcatg gatccgaatt ttactcttat catgaagtat
     1501 tgaaccaccc atgtaaaaaa gcacatctgc ttcatacctg aatgtaattt caacccgcac
     1561 atcaccctgt ccggcaggaa tactccatga gcctgacgca cttccgccaa catcagaata
     1621 taccggctca tatctgctgg cgtttttgga aatctggacc tcccgctccc cggttttgac
     1681 tttgcctgac ctggcatgaa cgacaacttt gtcatttatc ttcatgatga attcacctct
     1741 ggaaaatgaa tacgccataa gcataagctt gcgatcaaac ggcatgttgt ctttcagcaa
     1801 aatcattttc tgaaaagtca gctggttctg agtcattacg ccctgcccca tatcaaccgt
     1861 gtatcccttg acgatatcac caatgattcg gtcagcgctc atcgttccat taatggtgca
     1921 gtcttccgca atcgtcacgt tattcagcgt accggaactg gcactgatat tcccgctgat
     1981 atcggcatta cgggccgtca gcctgccgtc cggcgtcagg gagaatgcgg ggggattgcc
     2041 gccgctggta atggtggggg cggtaagaac ctttatcagg gcctcattga taaacgtctg
     2101 tcccccctgc gtgaccagtg cgggtgtggt atcaccattc tccggattaa tgaatgccac
     2161 acggtccgct gccagcagta tctggctctg catcccgtca ggggtgttct caataccggc
     2221 accgataccg gcaatatagc gacgaccatc ctgcatctgc tgtagcttca ccgcccacat
     2281 gctgttcagg tcattatttg tgtccgtctg cacacgctgg atttgctgta tggtggcgtt
     2341 ctggtcctcc agcgttttac tgaccgtctg cgtgatttca ttgcgggttt ctgtgatggt
     2401 ggtctgcatt tccgccatct cgtccttcag ctgactgttg tcaatctctg cccacagcgc
     2461 ctctgccaga tgcgtttttc ctatcttttc ccggaaaagt tccagatagc ccgcagcatc
     2521 attgctcgcc cgtccactgg cttccacaaa agcagattta cccaccaggt tgacgctgcg
     2581 cacataaaac cagaaatcct ttccgggctt aatgtgcggg ccggagacac tccactgact
     2641 gccggtcccc agataacggg cagaggtttc cacctgtgcg gcgtctgcaa tttttgcctc
     2701 cgaaaaccag aactcaaact gcaccgtcgg gtcatacacc gcaagacgcg gtaccgccgt
     2761 tatctgaaaa taccccggcg tcagttcaac accggcaggc gctgccggtg cgttaatccg
     2821 gaacgtggtg gtggcgggtt cgccctgctg gccatagctg ttaatcgccc tgaccgtcag
     2881 ggtgtattcc ccgggaggca ggccactgaa acggtgctcc gtatcggcag tgatggcggt
     2941 ggtcagcagg cggctgttct caccgcttcc actggtcagg cgcagactga agcgcacgcc
     3001 cttcaccacc cgcggcgtgt cccatttcgc cagcgccaga tactggccgt cagctgcgct
     3061 cacctccacc gtcaggtgct gcactgccgg tgggataacg ctgttcaggg tgcctgactg
     3121 cggctcaaag cgggctccgt tatccacaat ggcttctttt tccggtacgt gctgcaccgc
     3181 cgtgatggca aaggtgccgt ccgtgttttc ccggatggcc acacagcgga acaggcgacg
     3241 gcgcagtgac ggcagggaga gtccccacac cccgtatgtc tccacaccat caggcagggt
     3301 actgacctgt atccggtccg gcgcggggtg tgcggtgatg tccacactta ccggcttacc
     3361 gctgccgtta atcaggttca ccgtcgatgc acctgtcccc ggaagtgtca cttcacggtc
     3421 cagcgtcagg gtgcgggtgg cagcatcgat ggacaggaca cgtccgccgg tcagggtccc
     3481 ggcatagtca ttatcacaga tttcaataat gtcaccgggt gtgtgccgca gcccctgtga
     3541 cccgagcgtg aaatccaccg tctgcgtttc cagcagttcg gtctttatca cccacagtcc
     3601 ggcacggtgg gcctgaccgc ggctggtaca gccgaacgca tccatcttca gcaggttgcg
     3661 cccgtagcgc aggatggcgt ccgggtcttc caccagttca gtggaggtct gccagccgtt
     3721 ctgcgggtca gtgtaattca cctccaccgc cgtgtgccgg tccttcaggg cactgaagct
     3781 gtagcggaac cccacgccgt tatcatccac caccacatcg ctgttggtgt acggccacac
     3841 cacatccgac ggacggtcct gaacgaacgt cagcgtctgg ccgttccata ccggcataca
     3901 gcgcatcgcc gagcagaaat cccccaggac atcccagacc ttacgctgct gtgacaggta
     3961 cgcattaaag gtcatccgcg gctctgt
//
LOCUS       DAZFVO020000268         3895 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZFVO020000268 DAZFVO020000000
VERSION     DAZFVO020000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.270"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            346..1278
                     /gene="rfaD"
                     /locus_tag="ACHKNB_RS24490"
                     /old_locus_tag="pgaptmp_004898"
     CDS             346..1278
                     /gene="rfaD"
                     /locus_tag="ACHKNB_RS24490"
                     /old_locus_tag="pgaptmp_004898"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HGU4198253.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1288..2334
                     /gene="rfaF"
                     /locus_tag="ACHKNB_RS24495"
                     /old_locus_tag="pgaptmp_004899"
     CDS             1288..2334
                     /gene="rfaF"
                     /locus_tag="ACHKNB_RS24495"
                     /old_locus_tag="pgaptmp_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HGU4198254.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2338..3330
                     /gene="rfaC"
                     /locus_tag="ACHKNB_RS24500"
                     /old_locus_tag="pgaptmp_004900"
     CDS             2338..3330
                     /gene="rfaC"
                     /locus_tag="ACHKNB_RS24500"
                     /old_locus_tag="pgaptmp_004900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HGU4198255.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3327..>3895
                     /gene="rfaL"
                     /locus_tag="ACHKNB_RS24505"
                     /old_locus_tag="pgaptmp_004901"
     CDS             3327..>3895
                     /gene="rfaL"
                     /locus_tag="ACHKNB_RS24505"
                     /old_locus_tag="pgaptmp_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4198256.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYR"
BASE COUNT         1032 a          909 c          959 g          995 t
ORIGIN      
        1 aatgtcaaaa tatgcagtga taattgtggt ggatgatttc attactaata agaccattaa
       61 tataaaaaaa gaattatatc ataaatatta acagaatgta tactgtatat tatccattca
      121 aatgcttccg ttatttatca tacatcggaa tattgatact aaagcactat tcacatgcaa
      181 aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc aattagcatc
      241 cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat tacagacatt
      301 cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat catcgttacc
      361 ggcggcgcgg gctttatcgg cagcaacatc gttaaagccc tgaatgataa aggcatcacc
      421 gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct ggtggatctg
      481 aatatcgcag actatatgga taaggaagac ttcctgatcc agattatggc tggcgaagag
      541 ttcggcgatg tcgaagcgat tttccacgaa ggcgcgtgct cttccaccac cgagtgggac
      601 ggcaagtata tgatggataa caactatcaa tactccaagg agctgctgca ctactgcctg
      661 gagcgtgaaa tcccgttcct gtacgcttct tccgcagcca cctacggcgg acgcacctcc
      721 gactttattg aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta ctcaaaattc
      781 ctgtttgatg aatatgttcg ccaaatcctg ccggaagcga actcgcagat tgttggcttc
      841 cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc gagcgtcgct
      901 ttccatctca acacccagct taataacggt gaatcaccga agctgttcga aggtagcgag
      961 aactttaaac gcgacttcgt ctatgttggc gatgtggcag atgtaaacct gtggttcctg
     1021 gaaaatggcg tttccggcat cttcaacctc ggcaccggtc gtgcggaatc cttccaggct
     1081 gtagctgatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat tccgttcccg
     1141 gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa tctgcgcgcg
     1201 gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata catggcctgg
     1261 ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc gtcttgggtt
     1321 ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta tccccaggcg
     1381 ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat gccggaagtt
     1441 aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga acgccgcaaa
     1501 ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc taactccttc
     1561 aaatccgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg gcgcggcgag
     1621 atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg gccgctaatg
     1681 gtggaacgct atatagcgct ggcctatgac aaaggcatta tgcgcacagc acaagatctg
     1741 ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc atatacctgt
     1801 aatcaatttt cgctttcatc agaacgtccg atgattggtt tttgcccggg tgcggagttt
     1861 ggtccggcaa aacgctggcc acactaccac tatgcggagc tggcaaagca gctgattgat
     1921 gaaggttatc aggtggttct gtttggctcg gcgaaagatc atgaagcagg caatgagatt
     1981 cttgccgctt taaataccga gcagcaggca tggtgtcgga acctggcggg ggaaacacag
     2041 cttgatcaag cggttatcct gattgcagcc tgtaaagcca ttgtcactaa cgattctggc
     2101 ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg tccaagtagc
     2161 ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat taccggctat
     2221 cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat cgacattact
     2281 ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc ctgacggatg
     2341 cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt gcccgcactc
     2401 actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga agaagggttc
     2461 gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc aatacgtcgc
     2521 tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt tcgtgaagcg
     2581 ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa aagcgcggca
     2641 ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac cgctcgcgaa
     2701 cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca gcacgccgta
     2761 gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca aacccagggc
     2821 gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg cgaatatgcc
     2881 gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa
     2941 ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat
     3001 gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag
     3061 atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat
     3121 acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca
     3181 accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg ttctacaagt
     3241 atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct tgaaataata
     3301 accaataagt tgacatcgga gataagatga cctcaacatt atttttctct ctcgagaaaa
     3361 aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc acctattttt
     3421 tgggtgggat tacaaggtat aaacatctta ttgttattct tatgacaata acgacaatcg
     3481 tctatctctg caaacggcca aaacactatc tctcactatt taaaacattt ctttttggta
     3541 gtgttgccat attaactatt gctgcattgc tgtcacttct tcaatcccct gatgcaggtg
     3601 ctagcatgaa ggaaattttc aaagctatta ttgagaatac tttactatgc acaatagcaa
     3661 taccgatcat attgagagac gagaaaagag aagatgtcga aaaaatcgtt ttcttctcat
     3721 ttattagtgc gttgggctta cgctgttttt ctgaattgat tacctattat aaggactatc
     3781 aacaagggat aatgccattc gcagattata gacaccgtag catttctgac tcgatggtct
     3841 ttttattccc tgcattgtta aatctctggc ttatcaaatc agcaaaatac cgcat
//
LOCUS       DAZFVO020000269         3868 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFVO020000269 DAZFVO020000000
VERSION     DAZFVO020000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.271"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            179..460
                     /gene="drpB"
                     /locus_tag="ACHKNB_RS24510"
                     /old_locus_tag="pgaptmp_004902"
     CDS             179..460
                     /gene="drpB"
                     /locus_tag="ACHKNB_RS24510"
                     /old_locus_tag="pgaptmp_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HGU4198257.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            500..1195
                     /gene="yedJ"
                     /locus_tag="ACHKNB_RS24515"
                     /old_locus_tag="pgaptmp_004903"
     CDS             500..1195
                     /gene="yedJ"
                     /locus_tag="ACHKNB_RS24515"
                     /old_locus_tag="pgaptmp_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HGU4198258.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            1262..2680
                     /gene="dcm"
                     /locus_tag="ACHKNB_RS24520"
                     /old_locus_tag="pgaptmp_004904"
     CDS             1262..2680
                     /gene="dcm"
                     /locus_tag="ACHKNB_RS24520"
                     /old_locus_tag="pgaptmp_004904"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HGU4198259.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVVDAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2661..3131
                     /gene="vsr"
                     /locus_tag="ACHKNB_RS24525"
                     /old_locus_tag="pgaptmp_004905"
     CDS             2661..3131
                     /gene="vsr"
                     /locus_tag="ACHKNB_RS24525"
                     /old_locus_tag="pgaptmp_004905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HGU4198260.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3120..>3868)
                     /gene="yedA"
                     /locus_tag="ACHKNB_RS24530"
                     /old_locus_tag="pgaptmp_004906"
     CDS             complement(3120..>3868)
                     /gene="yedA"
                     /locus_tag="ACHKNB_RS24530"
                     /old_locus_tag="pgaptmp_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HGU4198261.1"
                     /translation="GHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
BASE COUNT          944 a          982 c         1079 g          863 t
ORIGIN      
        1 atcgctcaaa acgatttttc ctgtattcac tgccgttgcg caaaaattat ctatttgttc
       61 aaaaaatgag tgagtcttga ctggctcatc caatgtggaa aaatgtgact tttatcacat
      121 aatagtacta agtctgaatt ttccgggtta tctcaaaatg gaatacggtt cgacaaagat
      181 ggaagagaga ctctctcgca gccctggcgg caaactggct ttgtgggctt tctatacatg
      241 gtgtggctat ttcgtctggg caatggcgcg ttacatctgg gtgatgagcc ggatccccga
      301 tgcccccgtg tcgggatttg aaagcgattt aggctctacc gcgggaaagt ggttaggggc
      361 actggtcgga tttttattta tggctctggt cggggcattg ttaggcagca ttgcctggta
      421 cacccgacct cgtcccgcac gtagcagacg ctatgaataa aaactgctaa ggtggcgact
      481 ctttggcaac ggagcgcgta tggacttaca acactggcag gcacagttcg aaaactggtt
      541 gaaaaaccat catcagcacc aggacgcggc ccatgacgtg tgtcattttc gccgcgtctg
      601 ggctacggca cagaagctgg cggcagatga cgatgtcgat atgctggtga ttttaaccgc
      661 ctgttatttt cacgatattg tgagcctggc gaaaaatcat ccgcaaaggc agcgttcttc
      721 aatcctggcg gcagaagaga cgcgtcgcct gctccgtgaa gagttcgagc aatttccggc
      781 ggagaaaatc gaggccgttt gtcatgccat tgccgctcac agtttcagcg cgcaaatcgc
      841 ccccttaaca acggaagcta aaatcgtcca ggatgcggat cggctggaag ccctgggggc
      901 gattggcctg gcgcgcgtgt ttgccgtttc aggggcattg ggcgtggcgc tgtttgatgg
      961 tgaagatccg ttcgcacagc atcgcccgct tgatgataaa cgctacgcgc tggatcattt
     1021 ccagactaag ttgctgaagc taccgcaaac catgcaaacc gcaagaggca agcagctggc
     1081 gcagcacaac gcgcattttt tagtcgagtt tatggcgaag ctcagtgccg aactggcggg
     1141 ggagaatgaa ggtgtcgatc acaaggtgat agatgcgttt tcatccgctg gctgagcgcg
     1201 tggccctaaa tggctgtaat tatgttaacc tgtcggccat ctcagatggc cggtgaaatc
     1261 tatgcaggaa aatatatcag taaccgattc atacagcacc gggaatgccg cacaggcaat
     1321 gctggagaaa ctgctgcaaa tttatgatgt taaaacgctg gtggcgcagc ttaatggtgt
     1381 aggtgagaat cactggagcg cggcaatttt aaaacgtgcg ctggcgaatg actcggcatg
     1441 gcaccgttta agtgagaaag agttcgccca tctgcaaacg ttattaccca aaccaccggc
     1501 acatcatccg cattatgcgt ttcgctttat cgatctattc gccggaattg gcggcatccg
     1561 tcgcggtttt gaatcgattg gcggacagtg cgtgtttacc agcgaatgga acaaacatgc
     1621 ggtacgcact tataaagcca accattattg cgatccggcg acgcatcatt ttaatgaaga
     1681 tatccgcgac atcaccctca gccataaaga aggcgtgagt gatgaggcgg cggcggaaca
     1741 tattcgtcaa cacattcctg aacacgatgt tttactggcc ggtttccctt gtcagccatt
     1801 ttcgctggct ggcgtatcga aaaagaactc gctcgggcgg gcgcacggtt ttgcctgcga
     1861 tactcagggc acgctgttct ttgatgtggt gcgcattatc gacgcgcgtc gtccggcgat
     1921 gtttgtgctc gaaaacgtca aaaacctgaa aagtcacgac cagggtaaaa cgttccgcat
     1981 catcatgcag acgctggacg aactgggcta tgacgtggtt gatgcagaag ataacgggcc
     2041 agacgatccg aaaatcatcg acggcaaaca ttttctgccg cagcaccgtg aacgcatcgt
     2101 gctggtgggt tttcgtcgcg atctgaatct gaaagccgat tttaccctgc gtgatatcag
     2161 cgaatgtttc cctgcgcagc gagtgacgct ggcgcagctg ctggacccga tggtcgaggc
     2221 gaaatatatc ctgacgccgg tgctgtggaa gtacctctat cgatatgcga aaaaacatca
     2281 ggcgcgcggt aacggcttcg gttatggaat ggtttatccg aacaatccgc aaagcgtcac
     2341 gcgtacgctg tctgcgcgtt attacaaaga tggcgcggaa attttaatcg atcgcggctg
     2401 ggatatggcc aaaggtgaga aagactttga cgatccgcag aatcagcaac atcgtccacg
     2461 tcggttaacg cctcgtgaat gcgcgcgctt aatgggtttt gaagcgccgg gagaagcgaa
     2521 attccgcatt ccggtttcgg acactcaggc ctatcgccag ttcggtaact cggtggtcgt
     2581 gccagtcttt gccgcggtgg caaaactgct tgagccaaaa atcaaacagg cggtggcgtt
     2641 gcgtcagcaa gaggcacaac atggccgacg ttcacgataa ggccactcgc agcaaaaata
     2701 tgcgcgcgat tgccacgcgt gatacggcga tagagaagcg cctcgccagt ctgttaaccg
     2761 ggcagggcct ggcgtttcgc gttcaggacg ccagtctgcc cggacgcccg gattttgtcg
     2821 ttgatgaata tcgctgcgtg atatttaccc acggctgctt ctggcatcat catcactgct
     2881 atctgtttaa agtgcctgcg actcgaaccg agttctggct ggagaagata ggtaaaaatg
     2941 ttgagcgcga tcgccgcgat atcagtcgct tgcaggaact cggctggcgc gtattgattg
     3001 tctgggagtg cgcgttacgt gggcgcgaga agctgacgga tgaagcgctt agcgagcgtc
     3061 tggaagagtg gatctgcggc gaaggtgcca gcgcgcagat cgacacgcag gggattcatt
     3121 tactcgcttg atgcgtcctg aataactggc gcaactacgg gttttgccgg aaagagatat
     3181 tttcccaacg tgaccagcac caccgcgaag acaattacgc cgagcgccag ccattcaatc
     3241 ttcgacagcg tctctccgcc cagtcccgta cccagcaaga ccgcgaccac cgggttaacg
     3301 taggcgtagc tggtggcgag agccggactg acattacgga ttaaatacat ataagcgttg
     3361 atggcgataa tcgaaccaaa cagcgccaga tagccgaccg caaggaagcc tgaaagggaa
     3421 gggagcgccg tcagtttttc acctgcaatc atcgacgcga tcattaacac cacgcctgcc
     3481 gccagcatct caatagcacc cgccatcatc cctacaggta aggtaatgcg cgagccataa
     3541 accgagccaa acgcccagct aatcgagccg attaaaatca gaatcgcgcc ccacggattg
     3601 ccgcttaaat ttccaccgct attgagcatg atgattccgg caagcccaat ggcgataccc
     3661 acccattcca gtttgcgcgt tttaatgcca aacaggcggc tgaagcacag ggtaaagagg
     3721 ggcacggttg caaccacaac ggcggcgatg ccggaaggaa cattttgatg ctcggcaacc
     3781 gtcaccatgc cattaccgac agccagcaat aacaggccaa tcagcgcggc attgagcagc
     3841 ggacgtagcg gggggagctt gtgtccgc
//
LOCUS       DAZFVO020000270         3831 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFVO020000270 DAZFVO020000000
VERSION     DAZFVO020000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.272"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1006
                     /locus_tag="ACHKNB_RS24535"
                     /old_locus_tag="pgaptmp_004907"
     CDS             <1..1006
                     /locus_tag="ACHKNB_RS24535"
                     /old_locus_tag="pgaptmp_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4198262.1"
                     /translation="YVKGGVMYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLD
                     KPESRVKQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCV
                     GQGLHIAKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTA
                     LYEDLVARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSE
                     YSSQCVGNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRH
                     ELTNEPEEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL
                     "
     gene            complement(1321..1743)
                     /gene="subB"
                     /locus_tag="ACHKNB_RS24540"
                     /old_locus_tag="pgaptmp_004908"
     CDS             complement(1321..1743)
                     /gene="subB"
                     /locus_tag="ACHKNB_RS24540"
                     /old_locus_tag="pgaptmp_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HGU4198263.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(1760..2803)
                     /gene="subA"
                     /locus_tag="ACHKNB_RS24545"
                     /old_locus_tag="pgaptmp_004909"
     CDS             complement(1760..2803)
                     /gene="subA"
                     /locus_tag="ACHKNB_RS24545"
                     /old_locus_tag="pgaptmp_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HGU4198264.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            complement(3637..>3831)
                     /locus_tag="ACHKNB_RS24550"
                     /old_locus_tag="pgaptmp_004910"
     CDS             complement(3637..>3831)
                     /locus_tag="ACHKNB_RS24550"
                     /old_locus_tag="pgaptmp_004910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198265.1"
                     /translation="ISQAASNPDFYIINNTCFFHHQSVNQLNIFQGYFTLARQKLSGM
                     QDTLSGRAVVNAGTCFPKVI"
BASE COUNT         1106 a          836 c          851 g         1038 t
ORIGIN      
        1 ttatgtaaag gggggagtga tgtatcgtta tgattttaat gaaaaaatag atttctggta
       61 tgtgatagcc ctcgcaggac agtctaatgg tatggcctac ggtgaaggta ttcccctgcc
      121 agatacactc gacaaacccg agtcccgtgt aaaacaactg gctcgtcgaa aaacaatcac
      181 accaggagga aaagagtgta aattcaatga gattattccg gcagatcact gtctgcatga
      241 tgttcaggat atgagtggat atcaccatcc ggctgctgat ttgcataaag gggaatatgg
      301 ttgtgtcgga cagggattgc atatagctaa aaagttactg ccatacatac ctgagcaagc
      361 agggatatta ctggttcctt gttgcagagg aggtgccgcc tttactgttg gagctgaagg
      421 tatgtatgta cctgatacag gggcaacagc tgatgcaatg cggtggggca ccggaacagc
      481 actctatgag gacttagtag cccgggttaa ggtagctctg gaatataaca gaaagaataa
      541 actgctatct gtctgctgga tgcaggggga gtttgatctt atgtcaccag attatgaaaa
      601 gcatcctgac ttgttttatc agatggtaac ttctttcagg tctgagttat ctgaatactc
      661 atctcagtgt gttggtaatt cgtcagaaag agttccatgg ttgtgtggtg atacaacctg
      721 gtactggaag gaatcttatc aaaaagaata tgattttata tatggacatt acagacaacg
      781 gactgatgat gaaatacatt ttctcagctt tcaggatagt aaccgacatg aattaactaa
      841 tgagccagaa gaagatgccg atgatttatc tgtcgggtat ctggggtcat catggaggac
      901 agaattaagc tggacaacgt cacagagaag tacacacttt aattcaatgg ccagaagggg
      961 agttattgct gaatgttatg cgcaaaaaat aagaaattat ttgtagacaa gttcaggata
     1021 tatctacagc tctgacatga ttctctgaga gacatgacgg agtggtatgg agttgtcggt
     1081 atcaacacca agatagctgg tcgttaccgt ttgatatcac tggttctgat actatgtgga
     1141 cggtagtgtg tcacgaaggc acgcaccagg gacgtgagtc cctgtaatat catcgacagt
     1201 cccattatgg catgtctgca cctacaacgt catttcgagg tcggctcagt gttcactcga
     1261 gtcttatatc actgacgccg acactccatc cagagtatct ctggacggaa acatcaccta
     1321 ttagttcttt tttctgtcag ggccaaaaca ttcagtcgat gtggcgcagg tcgataaccc
     1381 aacaagggca ttggacgtta atgccttcac aaagggcgga tacgtccata ccccgggttc
     1441 ataataaatc ctgaccaact gacctgttgt gtaaaaatat agtgcctgat tatataatgt
     1501 ggaaaacgaa ggcccccaga cagatgaatt cttcatcgag caggctctta tggaagaacc
     1561 accaggttgt ttcccttcaa tacaaaaata agggttatta tctatttgcc ctgtatgaaa
     1621 ctgatcaata acaacgcctg aaaacatgcc atcccgggca tcgccagtcc actcagccat
     1681 ggcaggatta agggataaac atcccataac acctgcacat acaaaaaacc tcttaatcgt
     1741 catattcacc tccggaattt tacagttctt cactcatcct tccctgacgg acaggaatat
     1801 aatttttctt gcagaacatg ctaattgctt tttctgcatt aagaaccctc ccttccgtca
     1861 ccttatctgc aagtgagggg tatttgtctg cagactccag cagtgtacgc ttcagttctg
     1921 tcgctgttgc acggggatta catgagacca tcgctgccag aacgccggaa actatagctg
     1981 ttgcttccga ggttccacta cctgtccccg tttttccatc tggtctgagg aatgtaatat
     2041 tctgtcccgg tgcagcaata tcaacccagc cgtttccgaa ccgacttccg gtgatccctc
     2101 caccatgcaa tacaggtgtt tctcctttcc ggtactgtgc cagagctgcc actcttatca
     2161 caggatcccg ctttttattc atactgctga ccggatggta agccgcaggc catattctct
     2221 gctgagcact cagttttcgt atatctgcgc catcattacc cactgccgca acaattaatc
     2281 gctcattatt ccggcccata cgggaaagca gttcggtcca gaccgatgcg gaagatatcc
     2341 ccttctggcc tcccgatata ttaatgattt tctcttctcc tggtgcaaga aaaacatttg
     2401 acataatgct ttcagttgct ctaatccatg agtcctgtac accgtcagga ataacccttc
     2461 tgctggatat cagtgcatga ggataaacac cataaacccc atgacgtgag gcaatgagtg
     2521 aagccatggc agtaccatga atatataaag cttcagactc ttttaccggg aatggcgaac
     2581 catcctgagt aaaactgaat ttggcaaatt cgctgtcgct gaggcctcct acaaatgcta
     2641 ctccggaatc aacaacagat acgacaacag gggtattctt gtctgtgagc gacatggttg
     2701 tttctgtcag gccaattgca tcaaaatacc agggtttttc tgccctgaca gacacagata
     2761 taaaaagcag gaacagaata tgcggccata aaatcttaag cacaatatcc tccagtcagt
     2821 acaaataaac agtattgcag gtcaatatct tacaggaaca tcacatctga aatatcctgt
     2881 tttcgacaaa agataaacaa tcacaaccaa taaagcgagt gaagcccgtc tcaaaaacat
     2941 taatttttat atttatgtat ccaatgaatt aacgctcacc ttcacggtaa tatgcatctg
     3001 gatttctgca acaggtcagc gataagatgc tctgcctcct ccttcagagc agaagtaaat
     3061 acagccatga tggataaaac ttaaccagaa aaaataagag gaggcaagta agttgcctgg
     3121 catgtttatg acaataaaaa gttccgtcag gcatcatggc gaacaggata tcacagcgcc
     3181 gttttaccgg ggcaatcagt gaatggtggt gtggttcatt ggtaagcccc ggatatcagg
     3241 tcactgagta tattcatgaa tgaccgttaa accggggatg cgagctgctc cccgggaaag
     3301 tcgaactgac tggtcaatag atatcaatac ggggcagtca gtccacttac aggtatctga
     3361 caaggacacc ttctgagcgt gcaatgtgga cgggtaatac aacgatggta gcaggctggt
     3421 cagcaatcag cagtatatgc ccatatttcc tgaggccgga tatctgtttc ctgagcttgt
     3481 tttcgacgtt tgctaatatt ttgtcgaact aattatcaga acgattaatg ataacagtgt
     3541 ggcagggatc tttaccgata tcaacgtaaa taatgacctt aacaggtgcg taattgctgg
     3601 attttgccat gctgttaccc tgttgatacg ggttaattaa ataactttgg ggaagcaggt
     3661 accggcgttc actaccgctc tccctgataa tgtatcctgc atgcctgata acttttgccg
     3721 ggctaatgta aaataaccct gaaatatatt aagttgatta acagactggt gatggaaaaa
     3781 acaggtatta tttattatat aaaaatccgg atttgatgcg gcttgcgata t
//
LOCUS       DAZFVO020000271         3750 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFVO020000271 DAZFVO020000000
VERSION     DAZFVO020000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.273"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            161..412
                     /gene="rem"
                     /locus_tag="ACHKNB_RS24555"
                     /old_locus_tag="pgaptmp_004911"
     CDS             161..412
                     /gene="rem"
                     /locus_tag="ACHKNB_RS24555"
                     /old_locus_tag="pgaptmp_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HGU4198266.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQVLKEIGIKAGFSAFAEE"
     gene            479..757
                     /locus_tag="ACHKNB_RS24560"
                     /old_locus_tag="pgaptmp_004912"
     CDS             479..757
                     /locus_tag="ACHKNB_RS24560"
                     /old_locus_tag="pgaptmp_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198267.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            759..1808
                     /gene="ydfU"
                     /locus_tag="ACHKNB_RS24565"
                     /old_locus_tag="pgaptmp_004913"
     CDS             759..1808
                     /gene="ydfU"
                     /locus_tag="ACHKNB_RS24565"
                     /old_locus_tag="pgaptmp_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4198268.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            1822..2574
                     /gene="ydfT"
                     /locus_tag="ACHKNB_RS24570"
                     /old_locus_tag="pgaptmp_004914"
     CDS             1822..2574
                     /gene="ydfT"
                     /locus_tag="ACHKNB_RS24570"
                     /old_locus_tag="pgaptmp_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4198269.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGDADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(2749..2951)
                     /gene="cspF"
                     /locus_tag="ACHKNB_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /pseudo
     CDS             complement(2749..2951)
                     /gene="cspF"
                     /locus_tag="ACHKNB_RS24575"
                     /old_locus_tag="pgaptmp_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            3250..3465
                     /gene="cspB"
                     /locus_tag="ACHKNB_RS24580"
                     /old_locus_tag="pgaptmp_004916"
     CDS             3250..3465
                     /gene="cspB"
                     /locus_tag="ACHKNB_RS24580"
                     /old_locus_tag="pgaptmp_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HGU4198270.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            complement(3455..>3750)
                     /locus_tag="ACHKNB_RS24585"
                     /old_locus_tag="pgaptmp_004917"
     CDS             complement(3455..>3750)
                     /locus_tag="ACHKNB_RS24585"
                     /old_locus_tag="pgaptmp_004917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198271.1"
                     /translation="LRLWCFLWESSTYALCFAMPAVSFCCETIHAANLHFTTMVSILF
                     LIKILPNMLAIVSCIGPPYFLLKRSNIHYSVRISTQASAFFVIVYRRAMNFNQ"
BASE COUNT         1043 a          772 c          955 g          980 t
ORIGIN      
        1 gcacccgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc gtttgaagtt
       61 ctggacggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat aagagggatg
      121 gtcccttaaa ggggagagct aattatccgg aaggattctg atgatgaaca tcgaagaact
      181 gcgtaaaatt ttttgtgaag atggcctcta tgctgtgtgc gttgaaaatg gaaatcttgt
      241 tagtcattac cgcattatgt gtttgcgaaa gaatggggct gcgttaatta attttgtgga
      301 tggtcgagtg acagacggat ttatcttgcg cgaaggtgag tttgtcactt cattacaggt
      361 actgaaagag atcggaataa aagcaggctt ttcagctttt gcagaagaat aaactcatct
      421 acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat
      481 ggcacgcaac gtaaaatatt acaattctga taattcgccc gttcttgtct gcacgcacga
      541 gcggtattct cacgcattca agtctgaatg gttccaacac cctccatgca ctgaagagca
      601 ggctgaatgg ataattcagt gttaccgcag gcgcggatac gaggttaaga aagcccttag
      661 cctcgactac cgtcactgga taatctcagt cagattgcct tactccgaac gcccaccacg
      721 tgcgtcccgc actttccagc aacggatctg gaggtaacgt gcgggtatta cttagacctg
      781 ttctggtgcc tgagcttggg ctggtggtcc ttaagccggg ccgtgaatcc atacagatat
      841 ttcataatcc tcgagtgctg gtggaaccgg aaccaaaaag catgcgtaat ctgccatccg
      901 gagtcgttcc tgccgttcgc cagccgctgg cggaagacaa aacattgctg ccgtttttta
      961 gtaacgaacg ggtgattcgt gctgctggcg gtgttggcgc attgtctgac tggctattac
     1021 gtcatgttac atcctgccag tggcctaatg gcgattacca tcactctgaa acagtcattc
     1081 accgttatgg taccggcgca atggtgttgt gttggcactg cgacaaccaa ctgcgtgacc
     1141 agacatcgga atcactggag ctgcttgctc aacaaaatct gacagcatgg gtgattgacg
     1201 tcatccgtca cgcaataagc ggtacgcagg agcgggaatt atctttggct gaattatcct
     1261 ggtgggcggt ctgcaatcag gtggtggatg cactacctga ggctgtatcg cgtcgttcgc
     1321 tgggattacc agcggaaaaa atctgctcgg tgtaccgcga aagcgacatc gtaccgggag
     1381 agcagaccgc caccagcata ttgaaacaac gcacaaaaaa tcttgcaccg ttgccttacg
     1441 cccaccagca acaaaaaaca ccacaggaaa agacggtggt aagcatcacc gttgatccag
     1501 agtctccgga atctttcatg aagctgccta aacgtcgccg ctgggttaag gagaaataca
     1561 cacgttgggt taagacacag ccgtgtgctt gctgcggtat gccagccgac gatccgcatc
     1621 atctgattgg tcacgggcag ggcggaatgg gaacaaaagc acatgatctc tttgtgttgc
     1681 ctttgtgcag aaagcatcac aacgagctgc atacggatac agtggcattt gaagagaagt
     1741 atggctccca actggagctg atatttcgtt ttatcgatcg cgcgctggca attggcgtac
     1801 tggcgtaagt ggagaacgag catgaacctt gaagccttac caaaatatta ctccccaaaa
     1861 tctccaaaat tgagcgatga cgctccagcg acaggcaccg gttgtttaac aattacggat
     1921 gtaatggcag cgcaggggat ggtgcagtcg aaagcaccac ttgggttggc cttatttctg
     1981 gcaaaagttg gtgttcagga cgctcagttt gcgattgaag gcctgctaaa ttacgcgatg
     2041 gcactggata acccgacatt gaacaaattg agtgaagaaa tccggttaca gattattcct
     2101 tacctcgtga attttgcctt tgctgattac tccaggtctg cggcaagtaa ggctcgctgt
     2161 gagcattgtt caggtacggg attttataat gtattgcgcg aagtggtgaa acactacaga
     2221 cgcggggaat ctgtaatcaa ggaagaatgg gtgaaggaac tatgtcagca ttgccatggt
     2281 aagggcgaag tcagcacagc gtgcagaggg tgtaagggta aagggattgt tctggatgaa
     2341 aaaagaaccc ggtttcatgg cgtaccggta tataagattt gtgggcgttg taatggaaac
     2401 cggtttagtc gtttaccgac cacgctggca cgacgtcatg tccagaagct ggtaccagac
     2461 ctgaccgatt atcagtggta taagggggat gcggacgtca ttggtaaact ggtaacaaag
     2521 tgctggcagg aagaagcata cgcggaagcg caattgagga aggtgacgag ataaatgatt
     2581 tttgctgaag atggcgacat gatgtttgca tttttcaaaa aacatggata agattttctc
     2641 aacgatgggc tttgtgtatc cgacgtttag aaaaaagtag aaaacccgct tataagcggg
     2701 tttttgtgct ttaaatgggg caatagagat attgaatctc atcccgggat aaacattggc
     2761 agttgaaggt ccacgcgaac catttatcca gcaaaattcc acgcgtaatc ctgtggtaat
     2821 ttcttctgca tctcgaagat tgagagctga aacgtgaagc tgggcatcga tacgccatcg
     2881 gatgggaata taagaccttt gctgcttttg tagtcaaagt ttttgacaat tcctgtcatt
     2941 ttaggggaca gaaaaactcc ttaatactga taacctggtg caccatacac acgttcctgg
     3001 agaaaactac ttttttgata gggttgaagg tggctggatg tctaaaataa acattgcttc
     3061 atatgttcaa ctatgagtta atgactgtgt cggtttgaag aacagacgat atacgaagta
     3121 gtttactaaa gcagttctca tttcaggtgt tattcactta ttccttcttt gagtctctcc
     3181 aattaagtac gaagtcgttt ctgttatgca aaccatttat gccgaaaggc tcaagttaag
     3241 gaatgtagaa tgtcaaataa aatgactggt ttagtaaaat ggtttaacgc tgataaaggt
     3301 ttcggcttta tttctcctgt tgatggtagt aaagatgtgt ttgtgcattt ttctgcgatt
     3361 cagaatgata attatcgaac cttatttgaa ggtcaaaagg ttaccttctc tatagagagt
     3421 ggtgctaaag gtcctgcagc agcaaatgtc atcattactg attaaaattc atcgctcgtc
     3481 tgtatacgat aacgaagaag gctgatgcct gagtagagat acggacagag tagtgaatat
     3541 tggatctctt taataaaaag taaggaggtc caatacatga aacaatggcc agcatatttg
     3601 gcaaaatctt aatcaggaaa agtatgctaa ccattgtggt gaagtgcagg tttgctgcat
     3661 gaatagtttc acagcagaag ctaactgctg gcatcgcaaa acaaagtgcg taagtggatg
     3721 actcccacaa aaagcaccac aatctcaaac
//
LOCUS       DAZFVO020000272         3727 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFVO020000272 DAZFVO020000000
VERSION     DAZFVO020000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.274"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            92..724
                     /gene="yqeH"
                     /locus_tag="ACHKNB_RS24590"
                     /old_locus_tag="pgaptmp_004918"
     CDS             92..724
                     /gene="yqeH"
                     /locus_tag="ACHKNB_RS24590"
                     /old_locus_tag="pgaptmp_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4198272.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1058..1867
                     /gene="yqeI"
                     /locus_tag="ACHKNB_RS24595"
                     /old_locus_tag="pgaptmp_004919"
     CDS             1058..1867
                     /gene="yqeI"
                     /locus_tag="ACHKNB_RS24595"
                     /old_locus_tag="pgaptmp_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198273.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            1860..2341
                     /gene="yqeJ"
                     /locus_tag="ACHKNB_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /pseudo
     CDS             1860..2341
                     /gene="yqeJ"
                     /locus_tag="ACHKNB_RS24600"
                     /old_locus_tag="pgaptmp_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
     gene            complement(2374..2454)
                     /locus_tag="ACHKNB_RS24605"
                     /old_locus_tag="pgaptmp_004921"
     CDS             complement(2374..2454)
                     /locus_tag="ACHKNB_RS24605"
                     /old_locus_tag="pgaptmp_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198274.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(2490..2914)
                     /gene="yqeK"
                     /locus_tag="ACHKNB_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /pseudo
     CDS             complement(2490..2914)
                     /gene="yqeK"
                     /locus_tag="ACHKNB_RS24610"
                     /old_locus_tag="pgaptmp_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            3108..3598
                     /locus_tag="ACHKNB_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /pseudo
     CDS             3108..3598
                     /locus_tag="ACHKNB_RS24615"
                     /old_locus_tag="pgaptmp_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1319 a          645 c          630 g         1133 t
ORIGIN      
        1 tttcccagca aaattacaaa atcatggtgt attgacatca tcaatcacca atgaaattat
       61 gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct
      121 tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca
      181 gtactgcgca aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt ttaatcgtgg
      241 tgtggtagaa ggtatcttaa caaaaaacca caatgcgaga ttaagcggat atatttttgt
      301 cgatttttca gtaagttttt tacgtctatt ccttgaaaaa gactggattg actatcttgc
      361 aagtactgac atggggattg ttttagtcag cgaccgcaat atgcaatcgt tagctaatta
      421 ctggcgaaaa cacaactcag caatatctgc tgtcatatat aatgatgatg gtcttgatgt
      481 tgccaatgaa aagatcagac aactatttat tggtcgttat ttatcattta ccagaggtaa
      541 cacattaact cagatggaat tcaccataat ggggtatatg gtttctggtt ataatccata
      601 tcagattgct gaagttctgg atatggatat ccgtagcatc tacgcgtaca agcaacgaat
      661 cgaaaagaga atgggtggta aaataaacga attatttatt cgttcacatt cggtccaaca
      721 ttgatactca acgaccagcc agaatcatac tctgtttata cgggcattta ttgaaatgtc
      781 cgtattttat aaataggccg tcccctcaag agtaaacacc atagtattta aaaccatcac
      841 acgaaaaatt cagaagcatt acgaaatatg aatttacaaa caatagtggc ataaatgtta
      901 accatgttaa tttacgtaaa gttttacgtt gcaacattaa agcctcattt caatcatcat
      961 gataaatata aaattaatat atatttatgc cgtaaatacc aatatactta gcaaactatg
     1021 tgatctccat ttcgattgat ttagtgttta ttgacgtatg tactggatta ttaacgataa
     1081 tatcgagttc tggcctgagc accgaaaatt aatatcggta cataacgccg atcttaacgt
     1141 cgttctgaca acgccagcca gtcgatgttt atcacttcta cttgaagctt ttcctgatgt
     1201 ggttgcacaa caagattttt tcaccagagt ctgggaagaa gaaggtatgc gtgtgcctac
     1261 taacacgtta tatcagaaca tatccattat cagacgcgga tttcgcgctg ttggtgatac
     1321 tacccactcg ctaattgcaa ccgtgccgag aagaggattc aagatccata atgacattaa
     1381 catacaaaat catgtaataa actcgtcaac agacgcacat acacacaatg ccccacctgc
     1441 cataaaagtt aatgcgggat acaaagagag cattggtggc gcaaagaatt tcaataacaa
     1501 aatcctcaaa catataaaaa gtcatctaat tatgttgagc gcatttgtca taggcgcata
     1561 ttctgcatat tggctatgga ataataatca accgaagcca tttttcaagg attacaagac
     1621 tgttgcggaa attaatggct gccattttaa tgtaacagaa gatacaattg acgggttgaa
     1681 agagttcgat aaatataaaa cacggatact ggattcgggg attaactgta aaaaacatcc
     1741 gtggttatac ttccctcttg caaaatcctc accggggatg attgttatgg catgcaataa
     1801 aaattataac caacatgaag tggcagactg tttaaccctg tcttaccgtg aggttaatcg
     1861 tgattgatta taaaaaaaac ttattattta ttttagtttt tatcagtggg tttattctct
     1921 tcactgtcta ttcttacact gcagaaaaaa tgatatataa cgaaacatgc actgcaaatt
     1981 gggtaatttt taatgaccag ggacgagcaa atcttaccat cgactttatg tttaacaaaa
     2041 aaataaaaca ggaactgttg cgttgagtgg aacctggcaa caaggtaaca gagaaagtaa
     2101 atcaatacgg cgaaatattg aatacacatg gattgaaaac tatgacacag cccatttaac
     2161 atcaaagaaa gttaataagt ttgagattat ggatcaagtt gatgatgata gacttgtaca
     2221 acttattcct gatttttatg tctttccaga aaaaagtgta agctataata tactaaagca
     2281 aggtaagcat gcttttattt tgagcattgg taacagagca ataatgcatt gtgcaaggta
     2341 aactaaaata gataacaaaa agatggaacc tcgttaatca taaaacaata aaattaaata
     2401 ttctcgcagt atatggcagt ctaaagcatc aaagttttga tcaacatctt tcattttaga
     2461 catctccttg caatgtaaaa tatcatgtat caacaacatc tggtgcaaat ccatgagtcg
     2521 aactcgacat tcatcttagc tcgattatta ttccttcgta cagtcgatgt aaacaataca
     2581 gaaagaggat tattaagaac cagtttactc agtgaatcaa tagaggaaag gattaacgtt
     2641 tctttaaaag aattaatttc atcatctgtt aaactaaact catcattgac agatcgtgag
     2701 atataactgt ttttaacttt actctttacg ttgactttat tgacagaatt aacattcaca
     2761 tatcttgaat ttaatgtcca tgatgttgtt tgcgtgagaa ccttctcagc attaatgaat
     2821 tttttaacgg aatccttggt ttctttttcg agtccgaatt tacaatatca tgcatataga
     2881 ccatttcatg aatctgcgaa aactcaatgt ccattgtata cctcacattt ttaccgtgac
     2941 tcgatgttac tgttcaataa tcaccttcca tcaatactaa aattaatacc cctaatgtgc
     3001 cgataacaaa tatagtcatt ctacgtaacg tctccataag gtgatatttg acattatcag
     3061 aagctgcgaa ttcggatttt gctctaatca aatgagagaa atgtaaaatg aagccacgaa
     3121 atattaataa tagcctatca ctgcaaccat tagttcctga tcaggagaac aaaaataaga
     3181 aaaatgaaga gaaatccgtt aatccagtta aaatcacaat ggggtctggt ttaaattata
     3241 ttgaacaaga atctcttgga ggaaaatatc taatacatga tttgtcaata aagatagcgg
     3301 atatttctga agagataatt cagcaagcaa tattatctgc tatgagcata tataaatttt
     3361 cgataacaga tgatttaatg agtatggctg taaatgaact cataaaactg accaaaatag
     3421 agaataatgt agacctgaat aaattcacta ctatatgcac agacgttcta tcccccctcg
     3481 tcaccagaca taataaagaa aaaaaacaaa cgacattcta cccttcgcaa aaatcccctt
     3541 tttaattttc attgaaaaaa tggcaattga tactatgaat ccaggtcata cacattaagg
     3601 catttatgta atgtcgtaat taagacaact aataaggtga atattagtaa aacaactaat
     3661 gatgttatta ttttgtagtg aacagtactt ttaccaataa tgaaaaatat accataagca
     3721 attcaag
//
LOCUS       DAZFVO020000273         3634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFVO020000273 DAZFVO020000000
VERSION     DAZFVO020000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.275"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..299)
                     /locus_tag="ACHKNB_RS24620"
                     /old_locus_tag="pgaptmp_004924"
     CDS             complement(<1..299)
                     /locus_tag="ACHKNB_RS24620"
                     /old_locus_tag="pgaptmp_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198275.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSITDNA"
     gene            complement(292..3534)
                     /locus_tag="ACHKNB_RS24625"
                     /old_locus_tag="pgaptmp_004925"
     CDS             complement(292..3534)
                     /locus_tag="ACHKNB_RS24625"
                     /old_locus_tag="pgaptmp_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198276.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGRMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          680 a         1227 c          862 g          865 t
ORIGIN      
        1 ctggcgttat ccgtaatact ccactcccgg cacaccaccc tgaccgtccg gttatgtttc
       61 ggcggtcgcc acaaaaaggc acggtaacca ccatgccacg ataaaaattc atccagccag
      121 cgccgggtcg cctcatccgt cacccggaac actgcctgaa atgtcttcag tcgggcattc
      181 agtcccgtcg gtcggcgctg ttcataaccg tcaccaaacc ggaccctcac caccgacggt
      241 ttctcactca cctgcatccc ttcacgcggg accagatgca gcgtttttat ctcagccact
      301 cagcattcct ccgtctcgtc gcatggacag catcaccgcc tgcacccgct ggtcaatcaa
      361 ctgaacaagg ctgcctgccg cctccggccc tatctgaccg ttggccccgt cattctgaat
      421 ggcgatgtgg tagaccgggg aatacaccag acccgcactc ccgttcatcc tgcccacggc
      481 acgtacgccc agcgagccat ccgccgcccg ggtcaggggc attatggctt caggtccggc
      541 ttcccccatc agcccggccc cttttgcaaa ggcaaagtac gtgggcgtat ccacaatgct
      601 gttgctgtac gcactcaggt ttgccgaggt atacacgccg ccttttgcat tggccaccgc
      661 accgcccagc cagtcaccaa tactgccaat aaatcctcct gcaccggaca tactgtttgc
      721 cgccgtcttg atcccgttga caatcgcggc attcataaga acttttgata tttcctgcag
      781 cactgatgag gcccagttgc gccattccac tttattcccg ttcagcatct ccgtgatgtt
      841 attcaccatc cctgagatac cctccgtcgc aagctgtgct gcctgtgagg cgtaatcgga
      901 cgcattatcc acccagttac tgagcccctc ctgcaagcct ttctgccagt ccgcacgctg
      961 cgcatccgat tcggcataaa aggctgcctg gtccttaagg cgttcgctca gatactgcgc
     1021 gttctgtgcc cgtgcctgtc tgtaaaaatc ctcactgata tccccggtct gatactgaga
     1081 ctgaaggtcc gcatccttct ggcggaagct gtcgcggatc tgctgcaact cccgcatgcg
     1141 ttccctggct cgttctccct gcccgtatcc cagcagttcg gcttcatttg atgcacgcgc
     1201 agccacatta tcattcttca gggtctcttc ccgggatcgc aactgttccc ggattttttg
     1261 ctggtcaatc agggccgcgt tacgcagcag ttcctgcttc tgcatctccg tcagggtttt
     1321 cagttcgccc tgcgcagtct ggtacttcag cttcgccagc tctgtattct tacccgccag
     1381 tgccagttgc tctttctgct gcttcagtag ccgggaaaaa ctgtcttccg ctttttccgt
     1441 ctctgatttt ccaccccggg atttaggttt gttcgcctcg ttattacgcc aggcttccag
     1501 agcattactg atataacgct gtctcgcctc ctgatacgaa tcccccacaa aaccgaggtc
     1561 atccgccgca taccccagcc gggcacgctc tttttcctcc cccttcagtc gggacagggc
     1621 cagctcacgt tctgtttttg tcagggcgct ctgctgttta tcatccaggg tggcctgcgg
     1681 cagccgtaac ggtacattca ccagtccctg ccgctgctga agcagttcat tccccagccc
     1741 cagcagacgg ttgaattccg tatgctgacc gttcataacc agcatggact ggtacacctt
     1801 attctgctct gccgcctgct gacgaattaa cgccacacga cggtcttcca gcccggcaag
     1861 cacatcctga atggactgcg ctttttcctg catctgtgcc agacgggact gctcaacggc
     1921 aagctgctct gttgcctgag aaagcccttc cgttacggtc ttcaccgatg tcagatggtt
     1981 tatcatgaat ccgtcaccgg ttgtccagcc agggttagcc agaacatact gatatccagc
     2041 gattttttcc tgcagggatt tcacccggct ggcctgttca tcaatcagcc ggttctgctc
     2101 tgccagcgcc gcccgtgttc gtccttcatt atctgaggct tcaggcaaag acattgacgg
     2161 cgttttatgc gcgatttcat ctatcgtcag tgcatactgg cgcgctgact ccctggcctg
     2221 ctcctgattc tggtacagcg tataccatgc tgctgccccc agcatcacca gtccgggtac
     2281 gccaccaacc agtcccaacg caccagtcat cagacgtgag cccaccgccg ttgtactgtt
     2341 cagcgcattc tgggctgcgg ttctggcagc aatatttctg ttcaggcgtt cctgtgtggc
     2401 cgccagacgg gcttctgcag caatctgcat ctccgtcccg cgggctgccg ccacagcctg
     2461 ctgtgcacgg tacacggctg cccttgcccg cgccgtggca atctgcgtcc ccctgagctg
     2521 tgcttccgcc agtgccactt cattacgtgc tgccgtcaca agtcctgccg tggcagacac
     2581 cgctccggag gccatattgc caaagtaccg ggcaaccccg acggcaacca gcgcccccgc
     2641 tgctgttgcc acattatcaa tattaccggc aacaccgttc agcacgccgg agagcgtttt
     2701 tgtcacccca ctggcctcat tcgcgccacc cacccaggcc ataaaggcgt tttccacctt
     2761 tgtgatcccg tcagagaccg tttccggcat ggcggcatat tcatcacgca atacccccag
     2821 ctggctgatt aacgcaggaa cgactttatc cgccgtcagt ttgccgtcgt ccgccatcgc
     2881 cttaaggtct ttacgggcca cgcccatacc cgcagccagt gcacgtacga tccggtctcc
     2941 gctttcattg accgaattaa attcctcacc gcgtaacaca ccctgtgcca gcgcctggct
     3001 gaactgggtg atcaccgagc ccgcctctgc cgtactggca ccggagattt tcagccccgt
     3061 ggaaatggcc tccgtcacct tcagcacatc atcagcactg tagccatatt cacgcattga
     3121 ggctgccgag cgggcaaaca gggccgcatt atctgaaaat gccgtgcccg tccgctggct
     3181 gatatccatc agcactttct gtgatgacga aaattcatcg gatgactgcg acgcctgttt
     3241 cagtcgggca ttcacggaac tccattcatc ggccagagaa atcaggtgtc cggtggcaaa
     3301 ggcacctgca aatgcccccg ccgttccggc agctgaagcg cggatttccg tcaactggct
     3361 gttcagctca gccagggcgc gtcgctgctc ccgggcgact gcggcagcct gacgcccgcc
     3421 attctgcagg gtccggtaat attcactgcc catgcgggaa gcccgctgga tctccgactg
     3481 gaatgactgc gaatttgccg aaattttgat aatcagttca cgtaacgtcg ccattcacct
     3541 ttctccgggc gtaaaaaaac cgcctcagcg gttctcatca ttcatgactg tgctgcaaag
     3601 ctcagcgcgt cttccagcgc cgcaaacgga tcca
//
LOCUS       DAZFVO020000274         3632 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFVO020000274 DAZFVO020000000
VERSION     DAZFVO020000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.276"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..248
                     /locus_tag="ACHKNB_RS24630"
                     /old_locus_tag="pgaptmp_004926"
     CDS             <1..248
                     /locus_tag="ACHKNB_RS24630"
                     /old_locus_tag="pgaptmp_004926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198277.1"
                     /translation="IRCGYLQVSILTRQRNRKMKMFKGLTNEPETAFHHIAVLLEAGL
                     IISASSDEECDELSDDIFLLAQQYARSACDAFKEQRT"
     gene            245..445
                     /locus_tag="ACHKNB_RS24635"
                     /old_locus_tag="pgaptmp_004927"
     CDS             245..445
                     /locus_tag="ACHKNB_RS24635"
                     /old_locus_tag="pgaptmp_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198278.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            620..982
                     /locus_tag="ACHKNB_RS24640"
                     /old_locus_tag="pgaptmp_004928"
     CDS             620..982
                     /locus_tag="ACHKNB_RS24640"
                     /old_locus_tag="pgaptmp_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198279.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITRCVNH"
     gene            1390..1950
                     /locus_tag="ACHKNB_RS24645"
                     /old_locus_tag="pgaptmp_004929"
     CDS             1390..1950
                     /locus_tag="ACHKNB_RS24645"
                     /old_locus_tag="pgaptmp_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198280.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFYRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            1937..2521
                     /locus_tag="ACHKNB_RS24650"
                     /old_locus_tag="pgaptmp_004930"
     CDS             1937..2521
                     /locus_tag="ACHKNB_RS24650"
                     /old_locus_tag="pgaptmp_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198281.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKALEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            2545..3411
                     /locus_tag="ACHKNB_RS24655"
                     /old_locus_tag="pgaptmp_004931"
     CDS             2545..3411
                     /locus_tag="ACHKNB_RS24655"
                     /old_locus_tag="pgaptmp_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HGU4198282.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            3404..>3632
                     /locus_tag="ACHKNB_RS24660"
                     /old_locus_tag="pgaptmp_004932"
     CDS             3404..>3632
                     /locus_tag="ACHKNB_RS24660"
                     /old_locus_tag="pgaptmp_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HGU4198283.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGE"
BASE COUNT         1153 a          784 c          849 g          846 t
ORIGIN      
        1 cgatccgatg tggttactta caagtttcca ttctgacacg acaaaggaat cgtaagatga
       61 aaatgttcaa aggcctcacc aacgaaccgg aaacagcttt tcatcacatt gcagtactgc
      121 ttgaagcggg gttaatcatt tcggcttcca gtgatgaaga atgtgatgaa ctttcggatg
      181 acatattttt actggcacaa caatacgcca gaagcgcatg cgatgcattt aaggaacaaa
      241 gaacatgaaa actcccctga ttatgcttga ggaagtggca gcagaaataa aagaaaacac
      301 gtcaatgctt gagtttatat ttaaaaactc tggtgataac ggagagacag atgatttctt
      361 actttgtctg attcgttcca tgaataaaac ctgtgaaaag gcttacgagt atgttgatgc
      421 tctgagaacg aataaaggaa attaaacaga caatcataca taaaaacatg acggtctgcg
      481 gtcgggagtt ctcactattt taaaatgggg atgacacatg aaagacaaac atgcctataa
      541 aaccgcttta ttaatggctg atgccggata ctggtcagtt gcagtattgt ttctcaaaaa
      601 ggcatacggg aaataacaaa tgaaagatga atacatttct gcatatcgtc gccgcataac
      661 ggttgcagca ctgcggcgga tgaaacgaaa aacaggaagt aacctcctca ctgtaaagcg
      721 tcatgatgga gagttaacaa ctattgaaat aacagaacag ttcataagcc agttgctatt
      781 acgatttgaa ggcgttaccc gtggtgagtt tgggcggaaa gatggtgaaa cagcaatacg
      841 cactgcatac caggatgctg tcggtatcaa ccaacatggt gagtatctga ctgaatcagg
      901 aaagttaatc attgatgaac ttttgaatga atgcatcgat tacattaaag aggggcatat
      961 taccagatgc gttaatcatt aacagcatag ctcctctccc ggaataacgg agataatgaa
     1021 aaatgaataa ttgcgttgaa gtactcgata atgtccactg cgagataaca gaaagcctgt
     1081 ctttgttaca ggtgattatt gacagcggtg atattggttc accagatgcg ctaaacatga
     1141 tgaattgtct tttccgctca ttatcctgta cagcagacaa agcagaaaag catctgtgcc
     1201 ggactgaaaa cgaataacat tatttaagaa cagtaattaa tttacagctt tattgtcggg
     1261 gatttttaca acctgaaaag gccaggctgt atcacagata tatcgcaaca aacgaattgc
     1321 tgagccacat cttctggctg cgctcagagt gaataaaagt aattaacacc ggatgaacac
     1381 ggagtaacca tgaaagaaga acctgaaatc attgaaatta attctgaaga attcacagaa
     1441 aacgaatggc tgacagaaac cgatccgcgc gttctggcgc tgacaatgga aggatacatc
     1501 gtatttccac ctttcttcag tgatgcggaa tacagtgcaa taaataatgc cggaatcgac
     1561 cgtgcttttc ataaatacga actcgattgc ggccctgttt acagagtctg ccgccacgga
     1621 caagttaaga aatgcttcac gcgaaaaacg gccatcatac atctcgctca ttttatgaca
     1681 acgaaggtat tcgaaagttc aggttttgaa aaacgtctgc ctgaccagcg cctgatgcat
     1741 ccggaatatg gagaagtctt tcatccggga gaaattacgc ccgaatacta cagagctcac
     1801 gggcgttgtt tttatcgcct gttgcgcatc cttgcacgca gaaaacagtt tgatgcctgg
     1861 atggaaaaat acaacgcctg gattgatcag tattgccggc ttgtcgcgca aagaccattt
     1921 aagaagagag caaaccatga ctgcaactga acagttattt tttcttaccg ctgaaggtat
     1981 ccacacacct tacggactgc ttgatatcaa caaagcactg gaaatgctcg atgaaggctg
     2041 ttttgacgag ccgatctggc atggtctgga aatagtgtgt tgcatcagtg aagcagaaga
     2101 aaaaggcatg acaggtgagc tcgcgccgga aaagacaatc atgtactggc gctggcttta
     2161 taccgccatg ttcttctctg aacagctcac cagaaacggc tggaccgaag tgcttaacga
     2221 gaatggcggt acagacaagg gcaccatgta cagaggccac aacggtggca tgacaatcta
     2281 cctgaaacca ttacgtctcg ctatgcagaa ctatatggaa tgggcactca tagaaactta
     2341 cggagcggaa tcagggctgc tcaaaagcat tcttatgtac aaagcattca ccggcgctct
     2401 gggcgacggc gaaaaaaccg gcatcacaat ggaaggttac agagcgatgc acatgattct
     2461 ggatgggctt atcagtgaag ccactgacaa ctcagaaccg gaaaccagag tgttccacta
     2521 atcagctttt cgtgaggtgt aatgatgaaa aaatcaactg aacttgtgcc ggtggcagcc
     2581 cgtgatttgc aaattatcga atatcgcggt caacgcgttg tgaccactga acaactggcg
     2641 gcaggttatg gagcaacacc aatccgtatt cgccagaatc attcccgaaa cgaagaacga
     2701 ttcgttgttg gaaagcatta ttttttgctg gaaggcgatg aactacgtga acttaagcac
     2761 agagtatctc aaagctactc tgtgaaaatt gccagcaatg ttcgatccct catcctctgg
     2821 accgaacgcg gcgcagccaa ccacgcaaaa ttgctggaaa cggatcaggc gtggaactat
     2881 cacgaagacc ttgtggagtt ctacttcact cagcgggaaa acatcgatgt tcagcgcgta
     2941 ctcactcgca aggaactcgc gttgatggtt attgaggccg aagaacgggc tgaagctatg
     3001 gccctggaaa ataaaacgct ctctgcaacc gtagacagtc tggaaaaaca tttctctaaa
     3061 ggcatgactg ttccccagtt ctgccgtgcg ctgaacggcg taaataccag ccagataatg
     3121 tggtggctgt ctggccgaaa atgggtattc aacgaacaac gcgatccgga gaaaacaccc
     3181 cgctggcgca cggcctctta tgcccgtgac agatacctga ccgaagaagc agtacagatc
     3241 actccacacg ggaaagcaac attcacgaaa ttcacccccg tattgctgga aaaaggatgc
     3301 caccgtttat accagtttta catgaacggt gaactcccta tgaagaaaaa ctggaacggt
     3361 gaatttaaac acgacaaagc tatttacaca ccggaggcag aacatgaata gcactactgc
     3421 ggcaatcatg aactacgacc cgaacatgac attatgcggg cgcatggcaa gacagacggt
     3481 tcgtctgaca ctcgggcaat gggagtaccg cgaaacattt gaagtggacg ttgtcggcaa
     3541 tctgacggga ctggatgtaa taacctgcgc aattgaaaac ctttatgaaa acctgccgta
     3601 tgaagaagca caggatgaat ataccggtga aa
//
LOCUS       DAZFVO020000275         3581 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFVO020000275 DAZFVO020000000
VERSION     DAZFVO020000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.277"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2055
                     /locus_tag="ACHKNB_RS24665"
                     /old_locus_tag="pgaptmp_004933"
     CDS             <1..2055
                     /locus_tag="ACHKNB_RS24665"
                     /old_locus_tag="pgaptmp_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198284.1"
                     /translation="SSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQ
                     ASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQAS
                     AAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDAST
                     TKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTAQKGIVQLSNATNS
                     TSEMLAATPKSVKAAYDLANGKYTAQDATTTQKGIVQLSSATNSASETLAATPKAVKA
                     ANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQA
                     SSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAWVN
                     TSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTP
                     SEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGK
                     PASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTNNTGAHTHSISGTA
                     NSAGAHQHKSSGAFGGTNTSIFPNGYTAISNLSAGIMSTTSGSGQTRNAGKTSSDGAH
                     THSLSGTAASAGAHAHTVSIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVR
                     LA"
     gene            2055..2630
                     /locus_tag="ACHKNB_RS24670"
                     /old_locus_tag="pgaptmp_004934"
     CDS             2055..2630
                     /locus_tag="ACHKNB_RS24670"
                     /old_locus_tag="pgaptmp_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4198285.1"
                     /translation="MAFRMSEQPQTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(2728..3318)
                     /locus_tag="ACHKNB_RS24675"
                     /old_locus_tag="pgaptmp_004935"
     CDS             complement(2728..3318)
                     /locus_tag="ACHKNB_RS24675"
                     /old_locus_tag="pgaptmp_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4198286.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          980 a          852 c          910 g          839 t
ORIGIN      
        1 tcatctgcca gcgccgcgtc aacaagtgcc gggcaggcct cagccagtgc caccgccgcc
       61 ggaaaatcgg cagaaagcgc cgcatcatcc gcttcaacag ccacaacgaa ggctggcgaa
      121 gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac atccgaaacg
      181 aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtcagcc
      241 agttcggcgg cgtcatcggc atcatcggcg tctgcttcaa aagatgaggc gaccagacag
      301 gcgtcagcag cgaagggcag cgccacgaca gcatccacga aggcgacaga ggcagctggc
      361 agtgcgacgg cggcagcaca gagcaaaagt acggcggaat ccgcggcaac gcgcgccgag
      421 acagcggcaa aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg
      481 acgaaaaagg ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg
      541 gcaacgccaa aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag
      601 gatgcaacga cagcacagaa agggatagtt cagcttagca acgcgaccaa cagcacatct
      661 gaaatgctgg cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa
      721 tatactgctc aggacgctac gacaacacaa aaaggaattg tccagctcag tagtgcaacc
      781 aacagcgcat ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat
      841 gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc agctgatata
      901 acactgacgc cgaaagatat tggtactctt aactcaacaa caatgtcatt cagcggtggt
      961 gctggttggt tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttcaatt
     1021 acgttgattg gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa
     1081 cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc
     1141 acatcgacag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt
     1201 tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat
     1261 gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga agggttaacg
     1321 gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca
     1381 atcccgtggc catcagatac cgttccgtct ggctatgccc tgatgcaggg gcagactttt
     1441 gacaaatctg catacccgaa acttgcagcc gcttatccgt caggcgtgat ccctgatatg
     1501 cgtggctgga cgattaaggg caaacccgcc agtggtcgtg ccgtattgtc tcaggaacag
     1561 gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt ggggacgaaa
     1621 aacacatcgt cgtttgatta cggaaccaaa tccacgaata acaccggggc gcatacccat
     1681 agtattagcg ggactgcaaa tagtgccggt gcgcaccaac acaagagttc cggtgcattt
     1741 ggtggcacga acacgagcat tttccctaat ggttataccg cgatttcaaa tctaagcgcg
     1801 gggattatga gcacaacaag cggtagtggc cagactcgta atgcagggaa gacatcatca
     1861 gatggtgctc atacccactc gctgtccggc actgctgcaa gcgcaggcgc acatgcacat
     1921 actgtcagta ttggtgctca tacgcactcc gttgcgattg gttcacatgg acacaccatc
     1981 accgttaacg ctgctggtaa cgcggaaaac accgtcaaaa acatcgcatt taactatatt
     2041 gtgaggcttg cataatggca ttcagaatga gtgaacaacc acagaccata aaaatttata
     2101 atctgctggc cggaactaat gaatttattg gtgaaggtga cgcatatatt ccgcctcata
     2161 caggtctgcc agcaaacagt accgatattg caccgccaga tattccggca ggcttcgtgg
     2221 ctgttttcaa cagtgatgag gcatcgtggc atctcgttga agatcatcgg ggaaaaaccg
     2281 tctatgacgt ggcttccggc gacgcgttat ttatttctga actcggtccg ttaccggaaa
     2341 atgttacctg gttatcgccg gaaggggagt ttcagaagtg gaacggcaca gcttgggtga
     2401 aggatacgga agcagaaaaa ctgttccgga tccgggaggc ggaagaaaca aaaaacaacc
     2461 tgatgcaggt agccagtgag catattgcgc cgcttcagga tgctgcagat ctggaaattg
     2521 caacggagga agaaatctcg ttgctggaag catggaaaaa gtatcgggta ttgctgaacc
     2581 gtgttgatac gtcaactgca caggatattg aatggccagc actgccgtag ggtaaaacat
     2641 ataaattcta taattagatg tatctttcca tttacggcaa ggaagggggc ttggaacacg
     2701 taaagcatct cacaccgaga ttatttttta tatgtcaggt gtctgaagtt ttgctttggc
     2761 tcttaaaatg gtttgccgcg aggttttgaa ttcccgggca atggcactta tacttacacc
     2821 tgacttaatt cgttcgaata ccacctgttt ctgttcttca tttaacacag gtggtcgacc
     2881 aaaacgtttc cctgcgccgc gggctcttac tatcccggaa tgagtgcgtt caagtaaaag
     2941 gtctcgttca aattcagcga ctgctgaaat tacttgcatc atcatttttc ctgttggact
     3001 ggtcaggtca atgccaccca atgccaagca atgcactctg atacctgttt cggtcagttg
     3061 ttccactgtt ttcctgatat ccattgcatt acaaccaagg cgatccagtt ttgtcacaat
     3121 caattgatca ccacatttca ggcgagcaag caaccggtta aaaccaggac gctcactggt
     3181 tgctgctgag ccgctaatgt gttcttcgat tatttgctga ggtttgattt taaaacctgc
     3241 actttcgatt tcccggcgtt gattttcggt ggtctgatcc agcgttgata tccgacagta
     3301 agcaaaaatt cgagacatag tgagactcta tacgaaattg gtgttcatat cataatgcat
     3361 ctcagaaaat aattatgatt atttttgtgc atatttgtat gtacacgttc gaaaataaac
     3421 gaatgcgtat gcaaccccgt aattttggtg agacccaaaa ttgattttgt gaaaaatggc
     3481 tttaactcgg tttgtttttc gagttccggg cggactcaag gaagaagaat agtgttgcgt
     3541 gttattttaa ccagatttca agttgtttgg acgtggaaaa g
//
LOCUS       DAZFVO020000276         3533 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFVO020000276 DAZFVO020000000
VERSION     DAZFVO020000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.278"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..392)
                     /locus_tag="ACHKNB_RS24680"
                     /old_locus_tag="pgaptmp_004936"
     CDS             complement(<1..392)
                     /locus_tag="ACHKNB_RS24680"
                     /old_locus_tag="pgaptmp_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGU4198287.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANELKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAA"
     gene            complement(389..739)
                     /locus_tag="ACHKNB_RS24685"
                     /old_locus_tag="pgaptmp_004937"
     CDS             complement(389..739)
                     /locus_tag="ACHKNB_RS24685"
                     /old_locus_tag="pgaptmp_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGU4198288.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDCILNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(749..1075)
                     /locus_tag="ACHKNB_RS24690"
                     /old_locus_tag="pgaptmp_004938"
     CDS             complement(749..1075)
                     /locus_tag="ACHKNB_RS24690"
                     /old_locus_tag="pgaptmp_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4198289.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1072..3533)
                     /locus_tag="ACHKNB_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /pseudo
     CDS             complement(1072..3533)
                     /locus_tag="ACHKNB_RS24695"
                     /old_locus_tag="pgaptmp_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          734 a         1172 c          829 g          798 t
ORIGIN      
        1 gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg tggcatattc
       61 acggtcccca tttccacaaa ccgccagtag aaggcattgc ggggattatc cgccttcatg
      121 gtgttatcac tgttgccggt gtccggatta acaccacgga tatgcacacc ggattccatc
      181 ccgccatcgc gggagcgccg ggaaaggacc accacattgc ggcgcagttt tcccctgcgc
      241 accggtgccc gtgacaccac ttcttctttc agttcattcg cccccgcgcg ggttgcctca
      301 cgcagtaccc ggttgttttc tgcaccactc agaagctgca aatcacggct tatttcttca
      361 agtcccgaaa aatccagcag ggtttcgatc atttttcccc tcccagccga cagagaattt
      421 ccaggcgccc gccggtcgca tccggcacgg gcaccccgac aacattcagg atacagtcac
      481 gccagggacc actcagcaca tgaagtcgtg acgccgccgt gatttcccga ccggactgac
      541 cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc atccgctccc
      601 ggctgctcct gccccggata tccgcatgaa ttttcccgca tgacacccat tcttccgtca
      661 tttctccggc agcattacgg gttaacaccg ggttcagaac acttatcatc tgtgtcagac
      721 gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc aaatcgtaaa
      781 tcagcacgga cacagaaaac ggcagttccc cctgcacgag gtcttcccgc tcagcaagat
      841 ccggattccg gtacaacatc ccggtcaggc gcatggcagc ccccttcatc cgggttaatg
      901 cctcgcccgg gatcagttca ccgtcctcac ggattacctt atcccggctg ccctgaatgt
      961 aggccagcag cacagctgta gcctgacgaa ccttgtccat cagcatctca tcatccgcgt
     1021 catggtcaac acgcagatga gccttgattt cttccagtgt cagtaatgct gtcactttcc
     1081 acctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat gagcttctcc
     1141 gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg tcaccgtatc
     1201 gccggggtgg taggtttcac cggctctgaa cacaccgcga tagagcatca ccggcaggga
     1261 aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga acaaccgttc
     1321 acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc atccccgcat
     1381 cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat acgtgccccg
     1441 gggaaaggat ttttgatcgt caatggcagg gagtatttcc agtgccgtgg catcacgccc
     1501 gtcctgcgga gccggcaggg cattcaccgc ctccagaacc gccttccgca gaacatccgg
     1561 atcatagtca caaccgtcac gcggagcagg gatatgactt acggcctctt tcaccatctg
     1621 ctcaagcatc ggacgcacat catcgggggt aatacttttg ccgtccgtcg gtaccggaat
     1681 attcgcaacc gcatcattca ccgcctgctt cagtacttcc ggatcgtaat cacgaccatc
     1741 acgcggagca gggatatgac ttacggcctc tttcaccatc tgctcaagca tcggacgcac
     1801 atcatcgggg gtaatacttt tgccgtccgc cggtacagga atatttgcga ccgcatcatt
     1861 caccgccttc tgcaatactt ccggatcgta gtcacgacca tcacgcggaa caggaatatg
     1921 gcttaccgcc tccttcacca tctgttcaag catcggacgc acatcatccg gggtgagact
     1981 tttaccgtcc gccggctgcg gaatatttgc gaccgcatca ttcaccgcct gctgcagtac
     2041 atccggatca taatcacgac catcacgcgg taccggaatg gcccccacag cctcatccac
     2101 catcgcctgc agaaccggac gcacctcatc caccgtcaca tgcttctgta ataccacaga
     2161 cagggaagtc agtttctctt caaacgcttg tgcctgcgag gccatcttcc cctcaaatgt
     2221 gcgctgtaaa tccgccagca ccgcagcgaa ttcttcaccc agcgcacgga taatggacag
     2281 ttcacgctct gtcattttcg cagtatcccc ctgaacatcg ccttcaccgc atcacgctct
     2341 gtttcgctta tggccttatt accgtcagac gcgccctcct ggcgtgtgcc tgacgacgtt
     2401 ttcccggaag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc ctccagactg
     2461 tagttctgct gctgaagata cagtgcatcg cctcctgcca ggggcggcag attctcacgc
     2521 ttcctagcct cattgggcgt cagcagcgta tttttcaccg attcacccag tgttttcatg
     2581 cgccgttcgc tgtccattct cagcagcgtg gtgacatcaa attccgtact ctcgttttct
     2641 cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt ctgcagacac
     2701 tgggaataat actgctgctc cagcgcttcc acgttgtcac tggaaggcgg ttgtcccaca
     2761 ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagcggt catcttcagt
     2821 tgttccaccg tctgcgcatc caccggtgaa aacgtcgtgg ggttatattt cgccccgttg
     2881 ctcaggatcg ccgttttccc cgcattttcg cctgtatacc cgctgtccca gttgctcttc
     2941 agttttttcg cattttcttc cgtaatactg ccggggatct caatcacccc ggacggcctg
     3001 ccgccatttc tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg cgtggccgcc
     3061 agcccggcgg catacaccgg cggcagcccc acaagcggat gaaaaaaaca gttaaaccgg
     3121 tcgtggatca cttcccgggc aggcaccgtc accgcctccg tgatcccgca gttccggtcc
     3181 ggcgtgatgc gatagaacac ctcgccgtca tccgccacca gaggttcaac ccggctccag
     3241 tccagaatac gcagttcttt gatctgcccc cgggagttac ggattttcag caccaccgta
     3301 ttgccgtgac gcagtttggc gttcagccac agttcaaaaa actggatacg attctgctgt
     3361 gcattgggac gacgacagag acgggcaata tccccctgcc gtttttcacg gcggatcccc
     3421 tgtgtatccg tctgcatcag gcgcagccgc attttggcga tatcctggga tatcagcgaa
     3481 atgcaagaaa acaccgcatg aaaggagagg acactttccg gatcggcttt cac
//
LOCUS       DAZFVO020000277         3481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFVO020000277 DAZFVO020000000
VERSION     DAZFVO020000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.279"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..132
                     /locus_tag="ACHKNB_RS24700"
                     /old_locus_tag="pgaptmp_004940"
     CDS             <1..132
                     /locus_tag="ACHKNB_RS24700"
                     /old_locus_tag="pgaptmp_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4198290.1"
                     /translation="PSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            201..422
                     /locus_tag="ACHKNB_RS24705"
                     /old_locus_tag="pgaptmp_004941"
     CDS             201..422
                     /locus_tag="ACHKNB_RS24705"
                     /old_locus_tag="pgaptmp_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HGU4198291.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            498..875
                     /gene="cbeA"
                     /locus_tag="ACHKNB_RS24710"
                     /old_locus_tag="pgaptmp_004942"
     CDS             498..875
                     /gene="cbeA"
                     /locus_tag="ACHKNB_RS24710"
                     /old_locus_tag="pgaptmp_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HGU4198292.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            922..1299
                     /locus_tag="ACHKNB_RS24715"
                     /old_locus_tag="pgaptmp_004943"
     CDS             922..1299
                     /locus_tag="ACHKNB_RS24715"
                     /old_locus_tag="pgaptmp_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HGU4198293.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            1296..1784
                     /locus_tag="ACHKNB_RS24720"
                     /old_locus_tag="pgaptmp_004944"
     CDS             1296..1784
                     /locus_tag="ACHKNB_RS24720"
                     /old_locus_tag="pgaptmp_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HGU4198294.1"
                     /translation="MKLTLTPEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHA
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1796..1993
                     /locus_tag="ACHKNB_RS24725"
                     /old_locus_tag="pgaptmp_004945"
     CDS             1796..1993
                     /locus_tag="ACHKNB_RS24725"
                     /old_locus_tag="pgaptmp_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HGU4198295.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            2078..2923
                     /locus_tag="ACHKNB_RS24730"
                     /old_locus_tag="pgaptmp_004946"
     CDS             2078..2923
                     /locus_tag="ACHKNB_RS24730"
                     /old_locus_tag="pgaptmp_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HGU4198296.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     MLPSQ"
     gene            3157..3318
                     /gene="rhuM"
                     /locus_tag="ACHKNB_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /pseudo
     CDS             3157..3318
                     /gene="rhuM"
                     /locus_tag="ACHKNB_RS24735"
                     /old_locus_tag="pgaptmp_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          893 a          928 c          809 g          851 t
ORIGIN      
        1 cccagtaagg cagaccggct tatcaccgaa cgtctggtac aggcgctggc cctggttgac
       61 atccgggtgc cggaccatct gatagtcggt ggcagccagg ttttctcctt tgcggaacac
      121 ggtctgcttt aacctgtcac cgtcacaacc acatcatacc tgttttcgct tttatcttct
      181 gtcttcagag gtatcacact atgaaaatca tcacccgtgg tgaagccatg cgtattcacc
      241 aacaacatcc ggcatcccgt ctttttccgt tctgtaccgg caaataccgc tggcacggca
      301 gcactgaagc gtataccggt cgtgaagtgc agaatattcc cggtgtgctg gctgtatttg
      361 cagaacgccg taaagacagt tttggcccgt atgtccggct gatgagcgtc accctgaact
      421 gaatcagggc attcagatga gcagaattat cgccaccatc gaaccattct taactgattt
      481 tctgtgagga ctttatcgtg tcagacacac tccccggaac aacgcatccc gacgacaatc
      541 acgaccgccc ctggtggggg ctgccctgca ccgtgacgcc ctgtttcggg gcacgtctgg
      601 tgcaggaggg taaccgcctg cattaccttg ccgaccgcgc cggtatcaga ggcctgttca
      661 gcgacgcgga tgcgtaccac ctggaccagg cctttccgct gctgatgaaa caactggaac
      721 tcatgctcac cggcggtgaa ctgaatcccc gccatcagca taccgtcaca ctgtatgcga
      781 aagggctgac ctgcgaagcc gacaccctag gctcctgtgg ttacgtttat ctggctgttt
      841 atccgacacc ggcagcgccc gcaatcaccg tataaccccg gatatctccc ctttatcacc
      901 acctgttaca gagaacttaa gatgaataca ttacccgaca ctcacgtacg ggaggcatcg
      961 ggctgcccgt ctcccgtcac catctggcag acactgctca cccgactgct ggaccagcac
     1021 tatggtctca cgctgaatga cacaccgttc gctgatgaac gtgtgattga gcagcatatt
     1081 gaggcaggga tttcactgtg tgacgcggtg aactttctcg ttgaaaaata cgcactggtg
     1141 cgtaccgacc agccggaatt cagtgcctgt acccgttctc agttaataaa cagcatcgat
     1201 atcctccggg ctcgcagggc gaccggcctg atgacccgcg acaattacag aacggtaaat
     1261 aacattaccc aggggaagca tccggaggcg aaacaatgaa actgactctg acgccggaag
     1321 ctgacagcgt taacgtgcag gcgctgaaca tgggacgaat tgtcgttgac gtcgatggtg
     1381 ttaatctcgc tgaactgatt aacgtggtct gcgacaatgg ccacgccctt cgtgttgttg
     1441 atgaatctga ccgggtctca acagactgca cgccaccatt tgctgccctg accggcatac
     1501 gctgcagtac cgcgcatatc actgcaaagg acaacgcctg gctgtactcg ctgtcacatc
     1561 agacgaatga caccggcgaa tcagaatgga ttcatttcac aggtagcgga tatctgttac
     1621 gtaccgatgc gtggtcatac ccggttctgc ggcttaaacg cctggggctg tcaaaaacgt
     1681 tccgccgtct ggttgtcaca cttacccgac gttatggcgt cagtctcatt catctggatg
     1741 ccagcgctga atgcctgccg ggtttaccca ctttcgactg gtaaccagaa acaacatgaa
     1801 atcattaacc acggaaacag cgctggatat tctgattgcg tggctgcagg acaatatcga
     1861 ctgcgaatcg ggaattatct ttgacaacga tgaggataaa acggattcgg cagcactgct
     1921 gccctgcatt gaacaggcca gggaagatat ccgtacactg cgccagttgc agcttctgca
     1981 ccagaaccgg tgactctcac tcaccagact tcattccact gacgccagcc tgaacagggc
     2041 tggcgttttc atttatctgc aaaaaggaat atcgattatg tctgatatca cagtctcccg
     2101 tccggaagtt gtcaacgggc atacggacgt tatctgctca accagtatcc tccacattct
     2161 ggctgtacga aagagtacac tgctgcaaat cgacacactt atccggcaac tggctgaaat
     2221 ctcagcaatg acagaaagca ttggcggtaa aaccgcactg gactgggcca tgaaacagaa
     2281 ttttcactgc ggttgctggc tgatggagaa acctgaaacc gcaatgaaag ccatcacacg
     2341 caatctcgat cgcgaaatct ggcgtgacct gatgcaacgt tcagggatgc tttccttaat
     2401 ggatgcacag gcccgtgata catggtaccg gtcactggaa tacgataatt ttccggaaat
     2461 cagtaaagcg aacattctga gcacatttga acaactgcac cagaataagg atgaggtgtt
     2521 tgagcgaggg gtgatcaacg tcttcagggg actgagctgg aattacaaaa ccaactcacc
     2581 ctgtaaattt ggcagtaaaa ttatcgtcaa caacctagtg aggtgggacc ggtgggggtt
     2641 tcattttatc accgggcaac aggcagatcg acttgccgat ctggaaagaa tgctacacct
     2701 gttcagcggc aaaccgatcc ccgacaaccg ggaaaacatc acccttcgtc tggatgatca
     2761 catccagtct gttcagggta aagaggacta tgaagacgag atgttcagta ttaaatactt
     2821 taagaaaggc tcagtacaca tcacgttcag gaaaccagaa ctggttgaca ggctaaatga
     2881 tattatagcc agacactatc cggaaatgtt accttcacaa tagagtacta agaattgatt
     2941 caattatgta ttttagcttc attccttcat gcaaaatcat ttgggggttc ttatgggggt
     3001 tactcattta tccaacacat acaaattatt taagatcaat gcattaatac ttatattcga
     3061 ctcctgtgat ctccgccaaa atgcctctcc tgacgttctc caaccccttg taatactgtc
     3121 aatccagata ttttctactg ctcaatattc aatcggcttc aggaatacct ggtctaaggt
     3181 tttgtgatgg acgatgagcg gctgaagaat ccgcctgtcg gttcttctgt cctcgaacgc
     3241 atccgcgata ttcacgccag tatgcgccgt gtttatttgc aggtgatttg cccccctgct
     3301 gactctcagc caactgtatc gccattaagg tgaagatgtt gttttactcg ccccagaaca
     3361 ccctttttta tacgactatt ctatttcttt aaccgctggc gaaaaaaact gactataaac
     3421 ttcaccaata atcagactgc catcaagatt aatttgttca tttaatatat caaatcaact
     3481 t
//
LOCUS       DAZFVO020000278         3477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFVO020000278 DAZFVO020000000
VERSION     DAZFVO020000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.280"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            171..548
                     /locus_tag="ACHKNB_RS24740"
                     /old_locus_tag="pgaptmp_004948"
     CDS             171..548
                     /locus_tag="ACHKNB_RS24740"
                     /old_locus_tag="pgaptmp_004948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198297.1"
                     /translation="MRVLTPHQFFNLNWYVSNPELTSLKPAQDNEPALAMGYVWFHVQ
                     DEVNGAEPDEDMMCAVFPWYAEITTTDGTPDIQVFTEQDDCWLSAHQFMFARDAVPSH
                     DEYLVYFDSLNWRADVKKLLLDR"
     gene            601..1878
                     /locus_tag="ACHKNB_RS24745"
                     /old_locus_tag="pgaptmp_004949"
     CDS             601..1878
                     /locus_tag="ACHKNB_RS24745"
                     /old_locus_tag="pgaptmp_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HGU4198298.1"
                     /translation="MHTDNYVFELPATRGVQGGVEQYMLTVPMAVLRRLLAMDDQGDV
                     MARSQREANRTRARKIRNYVAEATGSGKPYILPSITGNIDIDVDFFPSELSPSVGILK
                     IPMEADLKLFDGQHRALGIMDFVRDYANTGDTISLLLTVGLPLEMRQQFFADINNNAS
                     KPAAAISMAYNNNDPVNQLAMYLAQNVSGLAGSVDFEHNAVPAKSSRLISFKALNDAT
                     KKMLSLRASSEPTLQQREAAEKLWSAWSGAMRWLDIAQDDIAAEYRQEALGLHGIMIN
                     AMGMATARMLQTRPIDSIVSLLACAEQGENGFHYRDSFMHENWHDICVDPQTGTIRTD
                     RRALEATAKELQRLVDPFAECAWLRHYLGDEVPDTTLLKFNAGIVKLKEETSLPQIAI
                     IEKLEQLRDGDEQTRSRIVGTLRAFKKYIAEGV"
     gene            2030..2455
                     /gene="ardB"
                     /locus_tag="ACHKNB_RS24750"
                     /old_locus_tag="pgaptmp_004950"
     CDS             2030..2455
                     /gene="ardB"
                     /locus_tag="ACHKNB_RS24750"
                     /old_locus_tag="pgaptmp_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdB"
                     /protein_id="HGU4198299.1"
                     /translation="METIEITARYISENARMNFMPAAFRGAFFSADHFIQSFLNRYAK
                     DYQGGYWEYLQASNGAFFMEAPQPLWLSLPNYFEGECSAREVGIIVCLYAYSYFCGLA
                     YEEGKAELNETMANRYHLLREYVNTLENESQNRIYRAID"
     gene            2513..2917
                     /gene="ardR"
                     /locus_tag="ACHKNB_RS24755"
                     /old_locus_tag="pgaptmp_004951"
     CDS             2513..2917
                     /gene="ardR"
                     /locus_tag="ACHKNB_RS24755"
                     /old_locus_tag="pgaptmp_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdAB regulator ArdR"
                     /protein_id="HGU4198300.1"
                     /translation="MLATLQHPTAWQLPARLMLLELLMFDYRNSDQERYGQQIYHHYR
                     KQGNHRWDTSVHQDSGGQYAIIFRHSFSKKQADGVKRTMIRDETVIRAGTAQELTEAT
                     YPAFQDSDILKASDFFKSLIQRKAADVTQKDI"
     gene            3029..3253
                     /locus_tag="ACHKNB_RS24760"
                     /old_locus_tag="pgaptmp_004952"
     CDS             3029..3253
                     /locus_tag="ACHKNB_RS24760"
                     /old_locus_tag="pgaptmp_004952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198301.1"
                     /translation="MKGSTKDKLSLAWLIVCIAISVGAGMVYWHYYQPGDGASIKSAY
                     ITWLSAFAVTGCTATILFCISGIARAFKNL"
BASE COUNT          871 a          902 c          908 g          796 t
ORIGIN      
        1 gtgtccggtg gagtccggcg cggtcgcctt ccatgccctg acgggcataa aaacaggtaa
       61 atatattccg tactcgtatt gactttaaag gcataatcac cgataattaa atcatcggga
      121 ggcaacaccg ccacccgcta aattgccggt atcggcagct aaggagattt atgcgtgtat
      181 taacccccca ccaatttttt aacctcaact ggtatgtcag taacccagaa ttgacctcac
      241 tgaaaccggc gcaagataac gaacctgccc tggcaatggg ttatgtatgg ttccacgttc
      301 aggatgaagt gaatggcgcc gagccggacg aggatatgat gtgtgctgtt ttcccctggt
      361 acgccgagat cactacaacc gatggcacgc cggacattca ggtattcacc gagcaggacg
      421 attgctggct ttccgcacat cagtttatgt ttgcgcgtga tgcggttcct tcccatgacg
      481 aatatctcgt ttatttcgac tcgcttaact ggcgcgccga cgtcaaaaaa ctgcttctcg
      541 atcgctaacc ttttcgcccg gataagtccg ggcataacct gaatgtgaag gaacctgaaa
      601 atgcatactg ataactatgt tttcgaatta ccggcaacac gcggcgtcca gggtggcgtt
      661 gaacagtaca tgctgactgt gccaatggcc gttttacgtc gccttctggc gatggatgat
      721 cagggcgatg taatggcccg cagccagcgc gaagcaaaca gaacgcgcgc gcgtaaaatc
      781 agaaactatg ttgccgaggc caccggcagc ggtaagccgt atattctgcc ctcgatcacc
      841 ggaaatattg atattgacgt agatttcttt cccagcgaac tatcgccgtc tgtgggtatc
      901 ctgaaaatcc caatggaagc agatctgaag ttattcgacg gacagcacag ggcgctgggc
      961 atcatggatt ttgtcaggga ctacgcgaat accggcgaca ccatcagcct gttgttgacg
     1021 gttggcctgc cgcttgaaat gcgccagcag ttttttgccg acatcaacaa caacgccagc
     1081 aaaccggcag cagcgatcag catggcttac aacaacaacg acccggttaa ccagctggcg
     1141 atgtacctgg cgcaaaacgt ctccggcctg gcggggtcgg tcgatttcga acataacgcc
     1201 gttccggcaa aaagctcacg cctcattagc tttaaggcgc ttaatgacgc gactaaaaag
     1261 atgctcagcc tgcgcgccag tagcgagcct acccttcagc agcgcgaggc ggccgaaaag
     1321 ctctggtctg cctggtcagg tgccatgcgc tggctggata tagcgcagga tgatattgcc
     1381 gccgaatacc gccaggaagc gctggggctt catgggatta tgatcaacgc gatgggtatg
     1441 gcaacggccc ggatgctgca aacgcggccg atagattcaa tcgtcagcct cctggcctgc
     1501 gcagaacaag gcgagaacgg ttttcactac agagacagct ttatgcatga aaactggcat
     1561 gacatctgtg tagatccaca aaccggcacc atccgaactg accgccgcgc gctggaagcg
     1621 acggccaaag agttacagcg gctggttgac ccgttcgcgg aatgtgcctg gttgcggcat
     1681 tatctgggcg atgaagtccc ggatacaacg ttgctgaagt tcaatgcggg gatcgtgaag
     1741 ctgaaagagg aaacgagtct gccgcaaata gcaattattg aaaaactgga gcagctgcgc
     1801 gacggtgacg aacaaacccg gtcgcgtatt gtggggacgc tgcgggcttt taaaaagtac
     1861 atcgcagaag gtgtatagcg agatggccac gcaggcgatg taaacaatat atgtaaatac
     1921 aattcgtact tatattgact ttagcgcccc attgtacgat aattaaaaag tggcaggtga
     1981 tgtgcctgcc acttaaccat cgccggtatc ggcttaaaga ggaagtatca tggaaactat
     2041 cgaaatcacc gcccgctaca tttctgaaaa cgcccgcatg aacttcatgc cagcggcctt
     2101 tcgtggcgct ttcttcagcg ctgatcactt cattcagtcc tttctgaatc gttacgctaa
     2161 agactaccag ggagggtatt gggaatacct tcaggccagc aatggcgcat tttttatgga
     2221 agcgccccaa cctctttggc tctcgttgcc taactatttt gaaggtgaat gcagcgcgcg
     2281 cgaagtaggg attattgttt gtctgtatgc ctacagttac ttttgcgggc tggcatatga
     2341 agaaggcaaa gccgaactga acgaaacgat ggctaaccgg tatcacctgt tgcgggagta
     2401 cgttaatacg ctggagaacg aaagccagaa tcgtatttat cgtgccattg attaactgat
     2461 aaccaggcgg gcaaagcccg ccttatcgtg actgtcacgc tcaggagaaa ctatgttagc
     2521 gacacttcaa caccctaccg cctggcaatt gccagctcgg ttaatgcttt tggaactgtt
     2581 aatgttcgat taccgtaatt ccgatcagga gcgctacggc cagcaaatct atcaccacta
     2641 tcgtaaacag ggtaatcatc gctgggatac ttctgttcat caggatagtg gcggccagta
     2701 cgcgattatt ttccggcact ccttcagcaa aaaacaggct gatggcgtta agcgaacgat
     2761 gatccgcgat gaaaccgtta tacgggcagg tacggcgcag gagctgacgg aagcgacata
     2821 tcccgccttc caggatagcg atattctgaa ggcgtcggat ttctttaagt cgcttatcca
     2881 gcgcaaagcg gcagacgtaa cacaaaagga tatttgaggg tcagccatgc tgaacaaaac
     2941 cgaaaaaaca ccaacattag tagcttcaat ccctgttcta cagagggttt aatcgccgta
     3001 aaggatgata aaggccacgg ggtaaagaat gaaaggttca acaaaagata agctgtcatt
     3061 agcctggctg attgtctgca tagctatatc tgtgggcgcc ggtatggtgt actggcatta
     3121 ttatcagccc ggtgacggcg caagtatcaa gagcgcatac attacctggc tttccgcttt
     3181 tgcggtgaca ggctgtacag caacaatttt attctgcatc agcggtatcg ccagagcgtt
     3241 taaaaatctt taaaagcgga accccggacg tcacccctcc ggggccgttc tgctgtgctg
     3301 cgcatcgagc cgctacgcgt ctcgccccgc tcacgcgggc agctgccact gacgcctccg
     3361 gcctgcggcc tccgcgctcc gcttggcaac cccttcccct tcagcctcct atcctaattc
     3421 gtctcctgca ttatctgtgg attgcgcagc tcagcggggc gctggccgtg tcggtgc
//
LOCUS       DAZFVO020000279         3427 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFVO020000279 DAZFVO020000000
VERSION     DAZFVO020000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.281"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..3372
                     /locus_tag="ACHKNB_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /pseudo
     CDS             1..3372
                     /locus_tag="ACHKNB_RS24765"
                     /old_locus_tag="pgaptmp_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
BASE COUNT          928 a          712 c          996 g          791 t
ORIGIN      
        1 aggaggcgtc gggacggtca atgttgaggg agaggactct gttctgacga ccgaattatt
       61 cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt acgtcacgag
      121 ttcaatcgtc gccattttag gctatcaggc gggcagtaat ggccaggttg tcgttgaaaa
      181 gggtggcgag tggctaataa aaaataacga ttcctcaatt gaatttcaaa ttggtaatca
      241 agggatgggg gaagcgacta ttcgcgaggg ggggctggtt acggctgaaa atacgattat
      301 cggtggcaat gccaccggtg tcggaaccct gaatgtgcag gatcaagact ctgtcatcac
      361 ggtacgcaga ctctataatg gatatttcgg taatggcgca gtcaatattt ccaataatgg
      421 actaattaat aacaaagaat attcattggt gggcgttgaa gacggttccc acggtgtcgt
      481 caacgtgacc gataaagggc attggagttt cctcggaaca ggcgaagctt tccgctatat
      541 ctatatcggt gatgctggtg acggtgaact taatgtctcg cgtgaaggta aagtagattc
      601 gggaattatc actgcgggga tgaaagaaac aggcacaggc aacattactg ttaaggataa
      661 gaactccgtt atcactaatc tcggaactaa tcttggttat gacggccacg gcgaaatgaa
      721 tatcagtaat gaggggcttg ttgtcagcaa cggaggaagc tcactcggtt atggagaaaa
      781 cggcgtcggg aatgtcagca tcaccacggg ggggatgtgg gaggtcaata agaatgtcta
      841 taccaccatt ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg gtaagtttgt
      901 atcgcaaaat attacttttt tgggcgataa agcaagcggt atcggcacac tgaacctgat
      961 ggatgggaca tcatcgttcg atactgtggg tatcaatgtc ggtaattttg gtagcggtat
     1021 cgtaaatgtc agtaatggtg ccacccttaa ttcaacgggc tatggattta tcggaggaaa
     1081 tgcctccggt aaagggatag ttaatatttc aacggatagt ctctggaatt taaagacatc
     1141 atctactaac gcccaattgc tacaggtcgg tgtattaggc acgggtgaac tgaatattac
     1201 caccggaggt atagttaaag cgcgtgatac acagatagct ctcaatgaca aaagtaaggg
     1261 cgacgtgagg gtggatgggc agaactctct tcttgaaaca ttcaatatgt acgtagggac
     1321 atctggtacg ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga
     1381 agtttactta ggtgtttttg aacctgctgt aggaacgcta aacattggtg cagctcacgg
     1441 tgaggcggcg gcagatgccg ggtatataac caacgcgacg aaagtagagt ttggttctgg
     1501 cgaaggtgtt tttgtcttta atcatactaa taacagtgat gccggctacc aggtcgatat
     1561 gctgattacg ggtgacgata aagacggaaa agtgatccat gatgcaggcc atacggtgtt
     1621 caatgcaggg aatacttata gcggtaaaac gctggtcaat gacggcctcc tgaccattgc
     1681 gtctcatacg gcagatgggg taacgggcat gggttcgagt gaagtcacca ttgcaagccc
     1741 cggtacgctc gacattctcg catcaacgaa cagtgcagga gattacacgc tgaccaatgc
     1801 gctcaaaggc gatggtttga tgcgagtgca gctgtcatcc tccgacaaga tgtttggctt
     1861 tacccatgca acagggactg aattcgccgg tgttgcccaa ctgaaagaca gtaccttcac
     1921 tctggaacgc gacaacaccg ctgcgcttac tcacgcgatg ttgcagtctg acagtgaaaa
     1981 taccacatcg gtaaaagtgg gagagcaatc cattggtgga ctggccatga atggcggtac
     2041 gctcattttc gatacggata ttcctgctgc gacgcttgca gagggatata tcagcgtcga
     2101 tacgctggtt gtcggcgcgg gtgactacac ctggaaaggc cgtaactatc aggtaaacgg
     2161 gacgggcgac gtgcttatcg acgtgcctaa accgtggaat gatcccatgg cgaataaccc
     2221 tctgacgacg ctcaatttgc tggaacacga cgatagccat gtcggcgttc aactggtgaa
     2281 ggcgcaaacg gttattgggt cgggtggctc attaacgtta cgtgatttac agggcgacga
     2341 ggtggaagcg gacaaaacgt tacacattgc gcaaaacgga acggtggtcg ccgagggtga
     2401 ttatggattc cgcctcacga ccgcaccagg tgatggtttg tacgttaact atgggctgaa
     2461 agcgctgaac atccatggtg ggcaaaagct gacgttagcc gaacatggcg gagcctatgg
     2521 cgcaacggcc gatatgtcgg caaaaatcgg tggtgaaggg gatctggcaa tcaatacggt
     2581 gcgacaggtt tcgctttcca acggtcagaa cgactatcag ggggcaacct acgttcagat
     2641 ggggacatta cgtaccgatg cggatggtgc acttggcaac acccgggaac tgaacatcag
     2701 caacgcggcc atcgtcgatc ttaatggatc tacgcagacg gtagagacat tcaccgggca
     2761 gatgggttcg actgttttgt tcaaagaggg ggcgctgacg gtaaataaag gtgggatcag
     2821 tcagggtgaa ctgacaggtg gcggaaacct gaatgttaca gggggaacgc tggctatcga
     2881 ggggcttaat gcacgctaca atgcgttaac cagcattagc ccaaatgcgg aagtcagcct
     2941 cgataatact caggggttag gcagaggaaa tattgccaat gacggtctgt taacgctaaa
     3001 aaacgtgact ggcgaactgc gtaatagcat aagcgggaag ggtatcgtga gcgcaaccgc
     3061 caggacagat gtagagctgg atggcgataa tagccgcttt gtggggcaat tcaacattga
     3121 tacaggcagc gcgctcagcg tcaacgagca gaaaaacctg ggtgatgctt ccgttatcaa
     3181 taatggcctg ctcaccatct ccactgagcg tagctgggcg atgacgcaca gtatcagcgg
     3241 tagcggtgat gtgacaaaac tgggtaccgg gatcctgact cttaacaacg attccgcggc
     3301 gtatcagggt acgacggata tcgtgggggg gaaattgctt tcggttccga ctctgccatt
     3361 aatatggcaa gttaacacat taatatccat aacagcggtg tgatgtcggg aaatgtcacc
     3421 actgcag
//
LOCUS       DAZFVO020000280         3401 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFVO020000280 DAZFVO020000000
VERSION     DAZFVO020000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.282"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..295
                     /locus_tag="ACHKNB_RS24770"
                     /old_locus_tag="pgaptmp_004954"
     CDS             <1..295
                     /locus_tag="ACHKNB_RS24770"
                     /old_locus_tag="pgaptmp_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198302.1"
                     /translation="PEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAV
                     EAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
     gene            296..1354
                     /gene="rsxD"
                     /locus_tag="ACHKNB_RS24775"
                     /old_locus_tag="pgaptmp_004955"
     CDS             296..1354
                     /gene="rsxD"
                     /locus_tag="ACHKNB_RS24775"
                     /old_locus_tag="pgaptmp_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HGU4198303.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1358..1978
                     /gene="rsxG"
                     /locus_tag="ACHKNB_RS24780"
                     /old_locus_tag="pgaptmp_004956"
     CDS             1358..1978
                     /gene="rsxG"
                     /locus_tag="ACHKNB_RS24780"
                     /old_locus_tag="pgaptmp_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HGU4198304.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1982..2677
                     /gene="rsxE"
                     /locus_tag="ACHKNB_RS24785"
                     /old_locus_tag="pgaptmp_004957"
     CDS             1982..2677
                     /gene="rsxE"
                     /locus_tag="ACHKNB_RS24785"
                     /old_locus_tag="pgaptmp_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HGU4198305.1"
                     /translation="MSEIKDVIVQGVWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            2677..3312
                     /gene="nth"
                     /locus_tag="ACHKNB_RS24790"
                     /old_locus_tag="pgaptmp_004958"
     CDS             2677..3312
                     /gene="nth"
                     /locus_tag="ACHKNB_RS24790"
                     /old_locus_tag="pgaptmp_004958"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGU4198306.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          804 a          873 c          906 g          818 t
ORIGIN      
        1 accagaagaa caggttgatc cgcgcaaagc cgccgtcgaa gccgctattg cccgagccaa
       61 agcgcgcaaa ctggaacagc aacaggctaa tgcggaacca gaagaacagg ttgatccgcg
      121 caaagccgcc gtcgaagccg ctattgcccg tgccaaagcg cgcaagctgg aacagcaaca
      181 gactaacgca gaaccagaag aacaggttga tccgcgcaaa gcggcagttg ccgcggctat
      241 tgcccgcgct caggccaaaa aagccgccca gcagaaggtt gtaaacgagg actaaatggt
      301 attcagaata gctagctccc cttataccca taaccagcgc cagacatcgc gcattatgct
      361 gttggtgttg ctcgcagccg tgccaggaat cgcagcgcaa ctgtggtttt ttggttgggg
      421 tactctcgtt cagatcctgt tggcatcggt tagtgctctg ttagccgaag ctctcgtact
      481 caaactacgc aagcagtcgg tagccgcaac gttgaaagat aactcagcat tgctgacagg
      541 cttattgctg gcggtaagta ttccccccct cgcgccatgg tggatggtcg tgctgggtac
      601 ggtgtttgcg gtgatcatcg ctaaacagtt gtatggcggt ctggggcaaa acccgtttaa
      661 tccggcaatg attggttatg tggtcttact gatctccttc ccggtgcaga tgaccagctg
      721 gttaccgcca catgaaattg cggtcaacat ccctggtttt atcgacgcca tacaggttat
      781 tttcagcgga cataccgcca gtggtggtga tatgaacaca ctacgcttag gtattgatgg
      841 cattagtcag gcgacaccgc tggatacatt taaaacctct gtccgtgccg gtcattcggt
      901 tgaacagatt atgcaatatc cgatctacag cggtattctg gcgggcgctg gttggcaatg
      961 ggtaaatctc gcctggctgg ctggcggcct gtggttgcta tggcagaaag cgattcgctg
     1021 gcatattccc ctcagcttct tagtaacgct ggcgttatgc gcaacgttgg gctggttgtt
     1081 ctcaccagaa acactggcag caccgcaaat tcatctgctg tctggtgcga ccatgctcgg
     1141 cgcattcttt attttgactg acccggttac cgcttctacg accaatcgtg gtcgtcttat
     1201 tttcggcgcg ctggcgggct tattagtctg gttgatccgc agtttcggcg gctatcctga
     1261 cggcgtggct tttgccgtcc tgctggcgaa catcacggtt cctctgatcg attactacac
     1321 gcgtccgcgc gtctacggcc atcgcaaagg gtaaaccatg ctgaaaacta tccgaaaaca
     1381 cggcattacg ttagcgctat ttgcagcggg ttcaacaggg ttaactgcgg ccatcaacca
     1441 gatgaccaaa acgacgattg ctgaacaggc cagtctgcaa caagaggcat tatttgatca
     1501 ggtgctgcca gccgaacgct ataacaatgc gctggcacag agttgctatc tggtaactgc
     1561 gccagagtta ggtaaaggtg agcatcgggt ttacatcgcc aaacaggatg acaaaccggt
     1621 agccgccgtt ctggaagcaa ccgcgccaga tggctattcc ggtgcgattc agctgctggt
     1681 gggagccgat tttaacggca cggtacttgg cacgcgcgtg acagagcacc acgaaacgcc
     1741 agggcttggc gataaaatcg aactgcgcct ttctgactgg atcacccatt ttgcgggtaa
     1801 aaaaatcagt ggtgcggatg atgcgcactg ggcggtgaag aaagatggtg gtgatttcga
     1861 ccagttcacc ggcgcgacga ttactccccg cgcggtggtt aatgcggtaa aacgcgccgg
     1921 attgtacgct cagacgttac cggcacaact ttctcaactt cctgcctgtg gagaataaag
     1981 cgtgagcgaa attaaagacg ttattgttca gggggtgtgg aaaaacaact ctgcgctggt
     2041 ccagttgctc ggcctttgtc ctctgttggc ggtcacgtcc actgccacta acgctctggg
     2101 tttaggactt gcgactacgc tggtactgac gctgaccaac ctgaccattt cgacgctgcg
     2161 tcactggacg ccagccgaga tccgcattcc catttacgtg atgatcatcg cctcggtggt
     2221 cagcgctgta cagatgctga tcaacgccta cgcctttggc ctgtatcaat cattagggat
     2281 ttttattccg ctgattgtca ctaactgtat cgttgtgggc cgcgctgaag ccttcgccgc
     2341 caaaaaaggt ccggcgcttt cggcactgga cggcttttca attggtatgg gcgcaacctg
     2401 cgccatgttc gtgctgggtt cactacgcga aattatcggc aatggcacat tgtttgacgg
     2461 tgcagatgcg ctgttaggta gctgggcaaa agtattacgc gtggaaattt tccacaccga
     2521 ctcccctttc ctgctggcga tgctgcctcc aggtgcattt attggcctgg gactgatgct
     2581 ggcaggaaaa tacctgattg atgaaagaat gaaaaagcgc cgtgctgaag caactgccga
     2641 acgtgcattg ccaaacggtg aaacagggaa tgtctgatga acaaagcaaa acgcctggag
     2701 atcctcactc gcctgcgtga gaacaatcct catcccacca ccgagcttaa tttcagttcg
     2761 ccttttgaat tgctgattgc cgtactgctt tccgctcagg cgaccgatgt cagtgttaat
     2821 aaggcgacgg cgaaactcta cccggtggcg aatacgcctg cagcgatgct tgaactgggc
     2881 gttgaagggg tgaaaaccta tatcaaaacg attgggcttt ataacagcaa agcagaaaat
     2941 atcatcaaaa cctgccgtat cttgctggag cagcataatg gcgaggttcc ggaagatcgt
     3001 gctgcgcttg aagccctgcc cggcgtaggt cgtaaaacag ccaacgtcgt attaaacact
     3061 gcattcggct ggccgactat tgctgtcgac acgcacattt tccgcgtttg taatcgtact
     3121 caatttgcgc cggggaaaaa cgtcgaacag gtagaagaaa agctactgaa agtggttccg
     3181 gcagagttta aagtcgactg ccaccattgg ttgatcctgc acgggcgtta tacctgcatt
     3241 gcccgcaagc cccgctgtgg ctcttgtatt attgaagatc tttgtgaata caaagagaaa
     3301 gttgacatct gaagaaaagg ggtaacaccg attaccccat tgataacctt tctttatcct
     3361 cttttaaaac atctttttaa cgtgaatgat gccattgctt a
//
LOCUS       DAZFVO020000281         3388 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFVO020000281 DAZFVO020000000
VERSION     DAZFVO020000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.283"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..178
                     /gene="ylcI"
                     /locus_tag="ACHKNB_RS24795"
                     /old_locus_tag="pgaptmp_004959"
     CDS             <1..178
                     /gene="ylcI"
                     /locus_tag="ACHKNB_RS24795"
                     /old_locus_tag="pgaptmp_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4198307.1"
                     /translation="TRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEACRRRLT
                     SEKRAYTSIKSDEE"
     gene            complement(343..549)
                     /gene="ybcW"
                     /locus_tag="ACHKNB_RS24800"
                     /old_locus_tag="pgaptmp_004960"
     CDS             complement(343..549)
                     /gene="ybcW"
                     /locus_tag="ACHKNB_RS24800"
                     /old_locus_tag="pgaptmp_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HGU4198308.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            835..1245
                     /gene="ybcV"
                     /locus_tag="ACHKNB_RS24805"
                     /old_locus_tag="pgaptmp_004961"
     CDS             835..1245
                     /gene="ybcV"
                     /locus_tag="ACHKNB_RS24805"
                     /old_locus_tag="pgaptmp_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HGU4198309.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1536..1829
                     /gene="bor"
                     /locus_tag="ACHKNB_RS24810"
                     /old_locus_tag="pgaptmp_004962"
     CDS             1536..1829
                     /gene="bor"
                     /locus_tag="ACHKNB_RS24810"
                     /old_locus_tag="pgaptmp_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HGU4198310.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(1920..2102)
                     /gene="rzoD"
                     /locus_tag="ACHKNB_RS24815"
                     /old_locus_tag="pgaptmp_004963"
     CDS             complement(1920..2102)
                     /gene="rzoD"
                     /locus_tag="ACHKNB_RS24815"
                     /old_locus_tag="pgaptmp_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HGU4198311.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2319..2816)
                     /gene="rrrD"
                     /locus_tag="ACHKNB_RS24820"
                     /old_locus_tag="pgaptmp_004964"
     CDS             complement(2319..2816)
                     /gene="rrrD"
                     /locus_tag="ACHKNB_RS24820"
                     /old_locus_tag="pgaptmp_004964"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HGU4198312.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2816..3031)
                     /gene="essD"
                     /locus_tag="ACHKNB_RS24825"
                     /old_locus_tag="pgaptmp_004965"
     CDS             complement(2816..3031)
                     /gene="essD"
                     /locus_tag="ACHKNB_RS24825"
                     /old_locus_tag="pgaptmp_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4198313.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1004 a          770 c          671 g          943 t
ORIGIN      
        1 aacccgcaga acaacaaccc gcaacatccg ctttcctaac caaatgattg aacaaattaa
       61 catcgctctt gatcaaaaag ggtccgggaa tttctcagcc tgggtcattg aagcctgccg
      121 tcggaggcta acgtcagaaa agagagcata tacatcaatt aaaagtgatg aagaatgaac
      181 atcccgcgtt cttccctccg aacaggacga tattgtaaat tcacttaatt acgagggcat
      241 tgcagtaatt gagttgcagt tttaccactt tcctgacagt gacagactgc gtgttggctc
      301 tgtcacaggt taagtagttt gaatgattag cagttatggt gatcagtcaa ccaccaggga
      361 ataatccttc atattattat cgtgcttcac caacgctgcc tcaattgccc tgaatgcttc
      421 cagagacacc ttatgttcta tacatgcaat tacaacatca gggtaactca tagaaatggt
      481 gctattaagc atatttttta cacgaatcag atccacggag ggatcatcag cagattgttc
      541 tttattcatt ttgtcgctcc atgcgcttgc tcttcatcta gcggttaaaa tattacttca
      601 aatctttctg tatgaagatt tgagcacgtt ggccttacat acatctgtcg gttgtatttc
      661 cctccagaat gccagcagga ccgcactttg ttacacaacc aatactatta attgaaaaca
      721 ttcctaatat ttgacataaa tcatcaacaa aacacaaaga ggtcagacca gattgaaacg
      781 ataaaaacga taatgcaaac tacgcgccct cgtatcacat ggaaggtttt accaatggct
      841 caggttgcca tttttaaaga aatattcgat caagtgcgaa aagatttaaa ctgtgaattg
      901 ttttattctg aactaaaacg tcacaatgtc tcacattata tttactatct agccacagat
      961 aatattcaca ttgtgttaga aaacgataac actgtgttaa taaaaggact taaaaaggtt
     1021 gtaaatgtta aattctcaag aaatacgcat cttatagaaa cgtcctttga taggttgaaa
     1081 tcaagagaaa tcacatttca gcaatacagg gaaaatcttg ctaaagcagg agttttccga
     1141 tgggttacaa atatccacga acataaaaga tattactata cctttgataa ttcattacta
     1201 tttactgaga gcattcagaa cactacacaa atctttccac gctaaatcat aacgtccggt
     1261 ttcttccgtg tcagcaccgg ggcgttggca taatgcaata cgtgtacgcg ctaaaccctg
     1321 tgtgcatcgt ttttaattat tcccggacac tcccgcagag aagttccccg tcagggctgt
     1381 ggacatagtt aatccgggaa tacaatgacg attcatcgca cctgacatac attaataaat
     1441 attaacaata tgaaatttca actcattgtt tagggtttgt ttaattttct acacatacga
     1501 ttctgcgaac ttcaaaaagc atcgggaata acaccatgaa aaaaatgcta ctcgctactg
     1561 cgctggccct gcttattaca ggatgtgctc aacagacgtt tactgttcaa aacaaacaga
     1621 cagcagtagc accaaaggaa accatcaccc atcatttctt cgtttctgga attgggcaga
     1681 agaaaactgt cgatgcagcc aaaatttgtg gcggcgcaga aaatgttgtt aaaacagaaa
     1741 cccagcaaac attcgtaaat ggattgctcg gttttattac tttaggcatt tatactccgc
     1801 tggaagcgcg tgtgtattgc tcaaaataat tgcatgagtt gcccatcgat atggtcagct
     1861 ctatctgcac tgctcattaa tatacttctg ggttccttcc agttgttttt gcatagtgat
     1921 cagcctctct ctgagggtga aataatcccg ttcagcggtg tctgccagtc ggggggaggc
     1981 tgcattatcc acgccggagg ccgtggtggc ttcacgcact gactgacaga ctgctttgat
     2041 gtgcaaccga cgacgaccag cggcaacatc atcacgcaga gcatcatttt cagctttcgc
     2101 atcagctaac tccttcgtgt attttgcatc gagcgcagca acatcacgct gacgcatctg
     2161 catgtcagta attgccgcgt tcgctagctt cagttctctg gcatttttgt cgcgctgggc
     2221 tttgtaggcg attgcgttat cacggtaatg attgaccgcc catgacaggc tgacgatgat
     2281 gcagataatc agagcggata taatcgcggt tactctgctc actgttgccc ccacaaacag
     2341 acttcacgct caatctcacg acgagtcatc aggcctttcc attgcttacc gccagcgtat
     2401 gtccagcgac gcagctgatc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga
     2461 agcgtcgatg ttctaaaatt gccagcaccc acattgtaaa cgaatgagta aagagcgccg
     2521 cgcgttgttt ccggtatatc gactttgata tacgggttaa tttgtctggc gacagtggca
     2581 aggtctttat tcaagagtgc tttgcattct gctttggtat acgttttacc gagcatgatg
     2641 tcttttccgg tgtgtccgtg acatacagtc catacaccaa caatatcttt gtatggtatg
     2701 tagctgacac cttccagacc atcgttacca cttgggccag tgattaacac tgatgctata
     2761 gcaattgctc cgccaccaat agcagcagca acggcttttc gtaatgatgg aggcattatt
     2821 cacctctcgc agccttgcgc ttatcttctt taatcttgaa ataaaggttt gtcaggtacg
     2881 tcagcaggcc aaataccagg ctacccagca cacctattgc tgcccactgt gagggagtga
     2941 ctttatcgag cagctgtaaa aaccagtaac cggcactacc tgctgaggtg ccataggcga
     3001 ctccagttgt taacttatcc atgaatttca taaccccacc tcgcagacaa agcgggtgta
     3061 aattgaggga atacaacgta tcgcaaaaaa gcagaaacgt aactgactcc gagtcagtga
     3121 ataactcagg tattgagtta tcagctaata tcgagactca aaaaatggaa aaaccagctc
     3181 gacggcgggt ttaagctgtg tgacgaagta accactctta acagcataac caatttttta
     3241 cgtacgtaaa ccactgaatg atatttatga gaatgctacc gagtgttcaa aacatcacca
     3301 caaatacata agaaaacctc aacaaataac caataaataa tttccagtgt tatttttagc
     3361 cggtttaaat taaacagacg aattatag
//
LOCUS       DAZFVO020000282         3349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFVO020000282 DAZFVO020000000
VERSION     DAZFVO020000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.284"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24830"
                     /old_locus_tag="pgaptmp_004966"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHKNB_RS24830"
                     /old_locus_tag="pgaptmp_004966"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKNB_RS24835"
                     /old_locus_tag="pgaptmp_004967"
     CDS             complement(209..736)
                     /gene="mobB"
                     /locus_tag="ACHKNB_RS24835"
                     /old_locus_tag="pgaptmp_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HGU4198314.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS24840"
                     /old_locus_tag="pgaptmp_004968"
     CDS             complement(718..1302)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS24840"
                     /old_locus_tag="pgaptmp_004968"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HGU4198315.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQVGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1372..1641
                     /locus_tag="ACHKNB_RS24845"
                     /old_locus_tag="pgaptmp_004969"
     CDS             1372..1641
                     /locus_tag="ACHKNB_RS24845"
                     /old_locus_tag="pgaptmp_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HGU4198316.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKNB_RS24850"
                     /old_locus_tag="pgaptmp_004970"
     CDS             1718..2704
                     /gene="srkA"
                     /locus_tag="ACHKNB_RS24850"
                     /old_locus_tag="pgaptmp_004970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HGU4198317.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2721..3347
                     /gene="dsbA"
                     /locus_tag="ACHKNB_RS24855"
                     /old_locus_tag="pgaptmp_004971"
     CDS             2721..3347
                     /gene="dsbA"
                     /locus_tag="ACHKNB_RS24855"
                     /old_locus_tag="pgaptmp_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HGU4198318.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          845 a          810 c          824 g          870 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcaga
      121 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
      181 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      241 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      301 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      361 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      421 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      481 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      541 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      601 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      661 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      721 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      781 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      841 ttctcctact tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      901 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      961 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
     1021 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
     1081 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1141 agcattaacc acgacgtgag agagctgcgc cataagcgcg tcagcgacat gttgccataa
     1201 tggtttgccg tttaattcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1261 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1321 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1381 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1441 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1501 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1561 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1621 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1681 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1741 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1801 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1861 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1921 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1981 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     2041 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     2101 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2161 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2221 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2281 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2341 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2401 ggatttgtgg atgttgctca atggcgataa agccgagcag tggatgcaac tggaaactat
     2461 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2521 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2581 cgcgttcccg aaaaattttc cgtggttaac cggggaagat tactggctgc gacagacggc
     2641 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2701 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2761 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2821 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2881 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2941 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     3001 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     3061 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3121 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3181 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3241 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3301 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaataatt
//
LOCUS       DAZFVO020000283         3331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFVO020000283 DAZFVO020000000
VERSION     DAZFVO020000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.285"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..1713
                     /locus_tag="ACHKNB_RS24860"
                     /old_locus_tag="pgaptmp_004972"
     CDS             193..1713
                     /locus_tag="ACHKNB_RS24860"
                     /old_locus_tag="pgaptmp_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HGU4198319.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1738..2076)
                     /gene="maoP"
                     /locus_tag="ACHKNB_RS24865"
                     /old_locus_tag="pgaptmp_004973"
     CDS             complement(1738..2076)
                     /gene="maoP"
                     /locus_tag="ACHKNB_RS24865"
                     /old_locus_tag="pgaptmp_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HGU4198320.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2195..3034
                     /gene="hdfR"
                     /locus_tag="ACHKNB_RS24870"
                     /old_locus_tag="pgaptmp_004974"
     CDS             2195..3034
                     /gene="hdfR"
                     /locus_tag="ACHKNB_RS24870"
                     /old_locus_tag="pgaptmp_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HGU4198321.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3130..3205)
                     /locus_tag="ACHKNB_RS24875"
                     /old_locus_tag="pgaptmp_004975"
     tRNA            complement(3130..3205)
                     /locus_tag="ACHKNB_RS24875"
                     /old_locus_tag="pgaptmp_004975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3170..3172),aa:Trp,seq:cca)
     gene            complement(3214..3290)
                     /locus_tag="ACHKNB_RS24880"
                     /old_locus_tag="pgaptmp_004976"
     tRNA            complement(3214..3290)
                     /locus_tag="ACHKNB_RS24880"
                     /old_locus_tag="pgaptmp_004976"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3254..3256),aa:Asp,seq:gtc)
BASE COUNT          889 a          820 c          816 g          806 t
ORIGIN      
        1 atttcagtat gaatgttgtt cccttcctcg tagttcatcc tttgtccgct gcatttttgc
       61 gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac aggaatcagc
      121 cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat aatggtgact
      181 caaggagggc ttatgtcact gtcaattgtt catacccgcg cagccctggg agtaaatgcg
      241 cccccgatca ctgttgaggt acatatcagt aaaggtctac ccggcttaac gatggtgggc
      301 ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc gcagcgccat tatcaatagc
      361 ggatatgaat atccggcgaa aaaaatcacc atcaacctgg ctccagccga tctgccaaaa
      421 gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc agaacagctt
      481 acagccaata agttagatga atatgaatta gtcggagaac tggctcttac aggcgctctg
      541 cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg cagaaaaatt
      601 atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg atgcctgata
      661 gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta agcacgctct cgaacgcccg
      721 aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt tgtcggtcag
      781 gaacaaggaa agcgaggact ggaaattacc gccgctggcg ggcacaacct tttactgatt
      841 gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct gttgccagat
      901 ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa tgctgaatca
      961 gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc gtcgttaact
     1021 gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc gcataacggc
     1081 gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc cttgcgagag
     1141 ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac ctatccagcc
     1201 cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca gggaaaccat
     1261 aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg gccctttctc
     1321 gaccgcttcg atctctcact ggagatccca ttaccacccc ccggcatttt gagtaaaacg
     1381 gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg taatggccgc cagagagcgc
     1441 caatttaagc ggcagaataa actgaatgcc tggctggata gtccggaaat acgccaattc
     1501 tgcaaacttg agagcgaaga tgcgatgtgg ttggaaggaa cactgatcca tctggggtta
     1561 tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa ccattgctga tattgatcag
     1621 tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc gattgaccgt
     1681 ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc ctttttatta
     1741 atcatcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt
     1801 gtgaaaacgt tttggacgct taatacgcgt catatacttg gaccacacgc gttctgcttc
     1861 tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt cctcggtaac
     1921 cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct caagcagttg
     1981 tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat gtttattgtc
     2041 gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta attctttgac
     2101 cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac aaaacattta
     2161 aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt taaaaacttt
     2221 cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct atctgaccca
     2281 gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga accttttcac
     2341 ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc cttatgcaga
     2401 aacgctcatg agcacctggc aggccgcccg taaggaggtg gcgcatacct cacgacataa
     2461 cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc agtggctggg
     2521 acgcttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc gaattgccca
     2581 acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta ttaccactga
     2641 agcgcccaaa atggacgaat ttagtagtca gttgctggga tatttcactt tagcgcttta
     2701 taccagtgcc ccttcaaaac taaagggaga tcttaattat ctgcgacttg agtgggggcc
     2761 agattttcaa cagcatgagg caggtttgat cggtgctgac gaagtgccca ttctgacaac
     2821 cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca cctggctacc
     2881 cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca caacactttc
     2941 acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatacgttga ttcgcgatct
     3001 tttgaaaatt aacgtgctgg atgaagtgta ttaatatgaa tggctggcaa ggatgccggt
     3061 agaaggattt acttcggaga gggttatttc agataaaaaa aatccttagc tttcgctaag
     3121 gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt ggagaccggt
     3181 gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga ctcgaacccg
     3241 cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc accgaattct
     3301 tttacaacca ccggttttat gaccggctta c
//
LOCUS       DAZFVO020000284         3067 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFVO020000284 DAZFVO020000000
VERSION     DAZFVO020000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.286"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            5..495
                     /locus_tag="ACHKNB_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /pseudo
     CDS             5..495
                     /locus_tag="ACHKNB_RS24885"
                     /old_locus_tag="pgaptmp_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371237.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            495..1010
                     /locus_tag="ACHKNB_RS24890"
                     /old_locus_tag="pgaptmp_004978"
     CDS             495..1010
                     /locus_tag="ACHKNB_RS24890"
                     /old_locus_tag="pgaptmp_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198322.1"
                     /translation="MFFKTSNPSALAAWQKYQQDYKKVKDEAKRLEAVLNVDCRSVFV
                     SGINGFYFKGLRFTDNKYPFHRDLWRKPTASNGWSCTPRTSCIPKALCAASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCGVQPEKQRMTEILSD
                     EFYAAEKRVRG"
     gene            1014..1565
                     /locus_tag="ACHKNB_RS24895"
                     /old_locus_tag="pgaptmp_004979"
     CDS             1014..1565
                     /locus_tag="ACHKNB_RS24895"
                     /old_locus_tag="pgaptmp_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4198323.1"
                     /translation="MMILQPMGRKGRAPAHVRPWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRLRFKPDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            1562..1894
                     /locus_tag="ACHKNB_RS24900"
                     /old_locus_tag="pgaptmp_004980"
     CDS             1562..1894
                     /locus_tag="ACHKNB_RS24900"
                     /old_locus_tag="pgaptmp_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198324.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHMGYEGFFIDEETGSCLEDEDGQVTHWCHISALP
                     ELQETPNG"
     gene            2032..2319
                     /locus_tag="ACHKNB_RS24905"
                     /old_locus_tag="pgaptmp_004981"
     CDS             2032..2319
                     /locus_tag="ACHKNB_RS24905"
                     /old_locus_tag="pgaptmp_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198325.1"
                     /translation="MEKEIKFAPKDIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFSR"
     gene            complement(2300..2539)
                     /locus_tag="ACHKNB_RS24910"
                     /old_locus_tag="pgaptmp_004982"
     CDS             complement(2300..2539)
                     /locus_tag="ACHKNB_RS24910"
                     /old_locus_tag="pgaptmp_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198326.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGVISREEATHIINIAQKS
                     AAPQSAEGHQDGSYINTNWHFDNLRACMGITAKKD"
     gene            2610..>3067
                     /locus_tag="ACHKNB_RS24915"
                     /old_locus_tag="pgaptmp_004983"
     CDS             2610..>3067
                     /locus_tag="ACHKNB_RS24915"
                     /old_locus_tag="pgaptmp_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4198327.1"
                     /translation="MNRASLITLIHVAKRDLQLDRETYISALLAATGKTSCRDMSPDE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTARIRAIWKVMHRQGFISDGAETALNRWV
                     KSQTAAQNGGEGVANWQWLEQHSALASDVLERLKRWHRRKMLAAMGMPERT"
BASE COUNT          755 a          678 c          830 g          804 t
ORIGIN      
        1 ctggctgcgt ttttctggcc gtctgaccag tcgttctctg gcagaaaaaa tgaatatgcc
       61 gctatcctcc atggtttatt ttctgcgtga tgcggtcgat gccggtgtgc tgacggaccg
      121 taacggtttt tatgatattc cgcgtccccg cccggtgcag ccggttcgtc gcaaatgcag
      181 ccaggagtct gcggctgatg atgttcagtg gtgcagcttc agaaaatccc tgccgtggat
      241 tgaagggcat gatattccgt cgatggcgtg ggaatttgct cagggcgttc tgacctgtga
      301 aaccgtttat gtggtggctg aagttgatga gcaggccatg aaagaaggcg tgccccagtt
      361 tgtgatggcg tatatcgaca ttcgcctggg tgtcattatc tgcggtttaa gcggctggaa
      421 tatcaccgaa catgttctgc gttacctgat tgttgaccgg acggctgcgc cgggatatct
      481 gcggaggtgg cgtaatgttc tttaaaacat caaacccttc cgcgctggct gcgtggcaaa
      541 aataccagca ggactacaag aaagttaagg atgaggcaaa acgtcttgag gccgtgctga
      601 atgttgattg tcggtcggta tttgtatccg gtatcaatgg tttttatttt aaaggactgc
      661 gttttacgga taacaaatat ccttttcatc gcgacttatg gcgaaaaccg actgcgtcga
      721 atggctggag ctgcacgccg cgcacatcgt gtattcccaa agccctgtgc gctgcctctg
      781 acgaacttaa cagtctgtgg cgtgaatatt cgcccgtcac gtatgccaga accgatgcac
      841 tgttgttctg gctgggcatt gacttctcag caatattgca tggtcccgtg aagtggttct
      901 gcgttgatga tgtgatttac cttcagtgtg gcgtacaacc tgaaaaacag agaatgaccg
      961 aaattctgtc tgatgagttt tatgctgctg aaaagcgagt cagggggtga tgtatgatga
     1021 ttttacaacc catggggcga aaaggtcgtg cacccgctca tgtccgtccg tggacacctg
     1081 aagaagatgc gctgctgatt gcgctttatc catccacccc ggttaaggat attgctgtca
     1141 gagtaaaaag aagtttctgg ggtgtacata accggattgt tttattacgc ggtacttacc
     1201 cggagttgct caaatgcaaa cgcctcagat ttaaacctga tgaagataaa tttatccgaa
     1261 aaaatgccag gacgatgacc ggtaagcaaa taggagaata tctggggcga gaccgggact
     1321 ctgtccacaa tcgggcgcga tatattggcg taagcatgaa aaaatacgga gagttgctgc
     1381 ccttcacccg catacctgac gatgatgttc gtcttattcg tgaattacgg gatgctgaat
     1441 caccacggcg tcttaccttt agggaaatcg gcgagaaatt tgaattatcc gagggcacgg
     1501 tgagttttat ttatcaccgt cgtcggactg ccgaagatgt tgtattacgg gagttaatgc
     1561 catgataacg acattatttg ttgaatcaga tgaacccctt gtgtgtgccg ccggaatgcc
     1621 ggtctgtggc ggaacgctga ccggtgttta tttcggggat ttgcgcggtt atccctggca
     1681 ttcactgaat gatgctttcc cgcctgatat ggaggctgtc gtgctgattg ttcagtatgg
     1741 tcacaggcag gaactgcgca tcggccatat ggggtatgaa ggctttttta ttgatgaaga
     1801 aactggatcc tgccttgaag atgaggacgg acaggtgacg cactggtgcc atatttcagc
     1861 tttaccggaa ttacaggaga cgcccaatgg atgatcaaat agttgaatgt cccacctgtg
     1921 gaaatgaagc cccggagtat ctgaaagagt gcccacattg tggtgaggta aaatgtaatc
     1981 actgcgatat gggcgacgac actgcatgta tgaattgtga ggattaataa tatggaaaaa
     2041 gaaatcaaat ttgcgcctaa agatatcgac gaagaactgg ctaaaatcgg catgcttgag
     2101 cgtatgcggg acattatcga atatgcgata aaagaaaatc ttgcagccag agaagccctt
     2161 ttgataatgg agcgggagat taacctgatt aaggatgctg tatctctgga taataaaata
     2221 gcccgcgagg aatacgttcg ccgcaggctc ggtgttgatg gttcagcaat tcttacatct
     2281 gaacattatg caaaaatctt taatcttttt tcgcggtaat gcccatgcag gcccggagat
     2341 tgtcgaaatg ccagtttgta ttaatatatg agccatcctg gtgtccctct gcgctttggg
     2401 gtgcggcact tttttgagca atattgataa tatgtgttgc ctcctcacgg gaaataaccc
     2461 ccttatcgaa cagggttacc ataagatttc cagccattgt gaatgcggca acatcaaggg
     2521 cattttttat atctgacata gatttttcct tttatgttaa ttgacatggc ggtgcggtaa
     2581 caccgcccat tttttatgga gccacgataa tgaatcgcgc ttccctgatt accttaatac
     2641 atgtcgcaaa acgcgatcta cagcttgacc gggagaccta tatatccgcg ctgctggcag
     2701 ccaccggcaa aaccagctgc cgggatatgt caccggatga gttatcccgc gtgctggatg
     2761 ttttcaaaaa acgcggtttt aaagtgcgcc agaagccggt taaccgggcc ttaaaaccgg
     2821 gtacggtgac cgccagaatt cgtgccatct ggaaggtgat gcaccggcag ggctttatct
     2881 ctgatggtgc ggaaaccgct cttaaccgct gggtgaaatc gcagacggcc gcgcagaacg
     2941 gcggcgaagg tgtggcaaac tggcagtggc tggagcaaca ctccgccctg gcctcagatg
     3001 tgctggagcg tctcaagcga tggcaccgcc gcaaaatgct ggccgcgatg ggaatgcccg
     3061 aacgcac
//
LOCUS       DAZFVO020000285         3044 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFVO020000285 DAZFVO020000000
VERSION     DAZFVO020000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.287"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..73)
                     /locus_tag="ACHKNB_RS24920"
                     /old_locus_tag="pgaptmp_004984"
     CDS             complement(<1..73)
                     /locus_tag="ACHKNB_RS24920"
                     /old_locus_tag="pgaptmp_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HGU4198328.1"
                     /translation="MNIENLKTKAEADISEYITKKIIE"
     gene            complement(245..475)
                     /gene="ynfN"
                     /locus_tag="ACHKNB_RS24925"
                     /old_locus_tag="pgaptmp_004985"
     CDS             complement(245..475)
                     /gene="ynfN"
                     /locus_tag="ACHKNB_RS24925"
                     /old_locus_tag="pgaptmp_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HGU4198329.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(480..545)
                     /gene="ynfR"
                     /locus_tag="ACHKNB_RS24930"
                     /old_locus_tag="pgaptmp_004986"
     CDS             complement(480..545)
                     /gene="ynfR"
                     /locus_tag="ACHKNB_RS24930"
                     /old_locus_tag="pgaptmp_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HGU4198330.1"
                     /translation="MKAPSGAFWLGVYSMDTHILR"
     gene            complement(548..736)
                     /gene="ynfQ"
                     /locus_tag="ACHKNB_RS24935"
                     /old_locus_tag="pgaptmp_004987"
     CDS             complement(548..736)
                     /gene="ynfQ"
                     /locus_tag="ACHKNB_RS24935"
                     /old_locus_tag="pgaptmp_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HGU4198331.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(747..959)
                     /gene="cspI"
                     /locus_tag="ACHKNB_RS24940"
                     /old_locus_tag="pgaptmp_004988"
     CDS             complement(747..959)
                     /gene="cspI"
                     /locus_tag="ACHKNB_RS24940"
                     /old_locus_tag="pgaptmp_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HGU4198332.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
     gene            complement(1322..1819)
                     /gene="rzpQ"
                     /locus_tag="ACHKNB_RS24945"
                     /old_locus_tag="pgaptmp_004989"
     CDS             complement(1322..1819)
                     /gene="rzpQ"
                     /locus_tag="ACHKNB_RS24945"
                     /old_locus_tag="pgaptmp_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HGU4198333.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
     gene            complement(1816..2349)
                     /gene="rrrQ"
                     /locus_tag="ACHKNB_RS24950"
                     /old_locus_tag="pgaptmp_004990"
     CDS             complement(1816..2349)
                     /gene="rrrQ"
                     /locus_tag="ACHKNB_RS24950"
                     /old_locus_tag="pgaptmp_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4198334.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            complement(2346..2656)
                     /gene="ydfR"
                     /locus_tag="ACHKNB_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /pseudo
     CDS             complement(2346..2656)
                     /gene="ydfR"
                     /locus_tag="ACHKNB_RS24955"
                     /old_locus_tag="pgaptmp_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            complement(2661..2876)
                     /gene="essD"
                     /locus_tag="ACHKNB_RS24960"
                     /old_locus_tag="pgaptmp_004992"
     CDS             complement(2661..2876)
                     /gene="essD"
                     /locus_tag="ACHKNB_RS24960"
                     /old_locus_tag="pgaptmp_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HGU4198335.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          885 a          687 c          567 g          905 t
ORIGIN      
        1 gttcaataat tttttttgtt atatattcag aaatatctgc ttctgctttt gtttttaagt
       61 tttcaatatt catcatttcc tcttttagtc tgttatgact ttccagttac acagtaagtc
      121 gattatatgg tgcaaacgtg taaaagataa gatgaaacat cgcaataatc aacatacgat
      181 agtctaaatt ttacacaaac agacaaagag aattttcctg aattatcaat gcaatagcat
      241 caaatcaact caagagcctt attgctgctt ccagaatttc ttctgaagta acatgtcgat
      301 ccgcggctac ataaatgact ttatgatctc cggtcagaga tggaaaccct gcggccatta
      361 cagtaaggtg tgttttttcg ccatttggat attcacgcat gatggtgtta actccagtca
      421 tcgctggcac taccactgct ggttcagagt taaaaaaaac tatgattttt ttcatgatgt
      481 taccgtagta tgtgagtatc catcgaatag acaccaagcc aaaaagctcc cgaaggagcc
      541 ttcattttca cttttttaaa tccaacgaca gacggctagc atttaagtat tgtgaaatat
      601 tatcaaatgt aatcatcatt gatttacaaa agatacattt tgccccgaaa ggattcatgt
      661 cagaaacatc aaaagatgat gttctatact gggaaccatg acaacacggg catctaaagt
      721 gaatatggtt tgtaatattg tctacctcaa agcgccacta catgaacagc ggcaggacct
      781 ttaggtccgt tctcaatacc aaattcaact tcctgattct cagttaatgt tttgaaatcg
      841 ttgctctgaa ttgctgagaa atggacaaac acatctttgc tgccatcttt cggcgtgatg
      901 aaaccaaaac ctttttcagg gttaaaccat tttactaaac cagtcatttt gttagacata
      961 attattacct tttgaagaaa ttagcccttg ggcagaatgg tccgaaaaaa aatatcagag
     1021 agaaaaacca acaaggaaat ctcaagaggt acaaataata aaattataac aatgactgct
     1081 tcagataaat ttgtaacaaa ccagaacacc attaacgcat gattaaccac ccatagcaag
     1141 gattactttt gtaaagaaaa acacagcaat gaaagaatag ctttatttat taataaaacg
     1201 tgtcattctg attaagacct tttatcttac ccttaagatt tcaggaattt tggctcatgg
     1261 aagagtcctt tttatttaaa ttttacattc cgcgatgtaa atgttccgat ttaatattac
     1321 cctacatttg atgcttttta tctcttaaag attcatagat ctgttgacaa gtcactcctg
     1381 cgatgtagcg ttcgtcagca atttcagcat aaagctgagc ttctgctgca atatctccga
     1441 gcatgttggt gagcattcct tcggcggttt tggttgtttt gcctctgacg gcagcggcaa
     1501 gatctgcggt atgcttcgct gcgtcaaggc gtatggcata tttttttgct tcggcacgca
     1561 actggttaac actatcagac agataagcag ccctggcaga aatttcagca gatttctgtt
     1621 gcgcatcttt aacagcctca tcacgggcta tagttcgccc ctgttcaatt attcgagcag
     1681 caaattgagc atttacctct tgtgataatg cggcagcatc acgttccgcc catttttttt
     1741 gccatcctcg gtcgctccag acatttccga cgataaatcc tgacaacacg agaaaaatca
     1801 ccatgaatat ctgattcact gttctatccc ccagcaggtt aatgcgctct cctggtcacg
     1861 acgaataacc tgaccgtaac agttatttga acgaatgcgg caatcgcgtc cgccatcctt
     1921 aatccaccag cgaatcgctt cgcatgcacc tttacgatca ccagcattca gccgcttata
     1981 aaacgtcgac gggaaacact taccggggcc aatgttatag ggacaaaatg acgcgatacc
     2041 cgctttttgt ggttcggtca gtggtacttt aatattgcgc tccacccatg ccagcgcctt
     2101 atcacgctca atggcgttga cctggtcgca tttttccttc gacagtttca tattgggaaa
     2161 aacggttttt ccatccacca ctgtggcacc ccgacagatg gtccatatgc cagaaccatc
     2221 gcggtatgcc attgtgtggt taccttcttt ttcgtccaga aactggtcaa gtatctgagg
     2281 agcagatgcg ccagcaccaa tcagcgccag aacggcagcc gacaggccgt atctgatttt
     2341 tgtgttcata gatatttatg atgaggacgc tcgtgcttat tggcaggatt ttcaatctta
     2401 aaggagtact gatgctgcag ataagactca acttttctga caatttttct gctacttcca
     2461 ggaagacttg ccggacgctc attctggctg ctgcctcata aaactccagc gtagctcctt
     2521 caacacggtc catggcgaca tccaggccaa aaatttcacc gtcaaagcgt tctttgtcct
     2581 gtaaggctac agttaccgta actttattct caaaattacg gactcctttc acaaccagtt
     2641 catagtcttg agtcattgga ttattctcct ctcgcagcct tacgcctgtc ttctttaatc
     2701 ttgaaataaa gatttgtcag atacgtcagc aggccaaaaa ccaggctacc cagcacaccg
     2761 attgcagccc actgtgacgg agttacttta tcgagtaact gcaatgccca gaaaccagca
     2821 ttacccgccg atgtgccata ggcaacacct gttgttaact tatccattga tttcatatcc
     2881 tcaccccgat gtacacggat ggtgcaatat gtttgaaaag atcggagtct acggggtagt
     2941 tttgacagca cacgttgttc tcaacggcgc taaaaaaaca tacacattaa aaatgtgggt
     3001 aattattttg aaagaaagtc atatataaaa taataatacg agaa
//
LOCUS       DAZFVO020000286         3032 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFVO020000286 DAZFVO020000000
VERSION     DAZFVO020000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.288"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKNB_RS24965"
                     /old_locus_tag="pgaptmp_004993"
     CDS             complement(31..1890)
                     /gene="gss"
                     /locus_tag="ACHKNB_RS24965"
                     /old_locus_tag="pgaptmp_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HGU4198336.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKNB_RS24970"
                     /old_locus_tag="pgaptmp_004994"
     CDS             2095..2961
                     /gene="yghU"
                     /locus_tag="ACHKNB_RS24970"
                     /old_locus_tag="pgaptmp_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGU4198337.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLARGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          715 a          817 c          768 g          732 t
ORIGIN      
        1 ataatcagta cggggttgct acaacatgta ttactttttc accacaatta acggttcaat
       61 atcactctct tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt
      121 gccgcccacg gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca
      181 cagttgctga tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc
      241 ttcatgatgg ctgaccaggt cgatattgct accacagcga ccggcaatcg gtttcactgc
      301 gtaacctgtt ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg
      361 gtgcgggaac agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca
      421 cagcggctca aagactagca cttccgggcg cagcaatacg tcgataagac gcacttcgtt
      481 ttgcggatga ccggtacgga ttggcaccgc agcaaactca cggtcgctaa cttcacgaat
      541 ctgatcaaac gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc
      601 cccatcaatc agttgcccgg cagcatccca gcccagttca tccaatccac gcaagatacg
      661 cgtttcaaag cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc
      721 gatatctttg tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc
      781 agccaattcg ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc
      841 cgcccaacgt tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta
      901 ctcgtaaacc ttcaggccac gctcatccat gcagaaatcc atacgaccag tgatcatatg
      961 gtgacggcga cgctgccagg agagacgcaa acgtggccag aggattttcg ggatgtcgaa
     1021 cagcgccagc aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg
     1081 cagctcgttg gtggctttaa ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg
     1141 ataaggatcc tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc
     1201 tttttcatcc agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat
     1261 tttcagcagc tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg
     1321 gatcatccag cccagaatgg tggtgtcatc aaaagtgtct ttcagggtat agcagccgtt
     1381 ttcgaccacc atctccagct cacgcgtcca ctgttgccct tgcggcaacg gggaatgaat
     1441 cacgttctgt tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg
     1501 gccagtgtct ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc
     1561 acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg
     1621 caggaagcgc agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta
     1681 attcagaaag agaaaacggc gagcaaattc aacgcattgc cacttgtggc ccatatattc
     1741 gtcgtcgata tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact
     1801 gtaatctgaa gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg
     1861 ggcatcctgg ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca
     1921 gttggcgtaa ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag
     1981 actaatcata cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta
     2041 cagcaagtga cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca
     2101 gacaatactt atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc
     2161 gccaatatca atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa
     2221 cacccattgc aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt
     2281 gaggagctgc tggcgcgggg cgttactggt gcagagtacg acgcctggct gattcgtatt
     2341 ggcgatggcg atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg
     2401 gcgctgcgcg atcatacgca taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg
     2461 ctttatctgg ctgagaaatt tggctacttc ctgccgcagg atttggcaaa gcgtactgaa
     2521 acgatgaact ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt
     2581 cacttttacc attacgcgcc gatgaaaatt gagtacgcca tcaaccgctt taccatggaa
     2641 gccaaacgtc tgctcgacgt gctggataag caactggcgc agcataagtt tattgcgggc
     2701 gatgagtaca ccattgcgga tatggcgatt tggccgtggt ttggcaatgt ggtgttaggc
     2761 ggcgtgtatg atgccgcaga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg
     2821 gcgaaagaag taggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac
     2881 ggaccgctga atgagcagtt gcatgagcgc catgacgcca gtgatttcga gacgaatacg
     2941 gaagataagc gtcaggggta agggttggtg ttcgtcgcag caagccatcc aggccggata
     3001 aggcgttcac gccgcatccg gcaatcgtgc ac
//
LOCUS       DAZFVO020000287         2991 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFVO020000287 DAZFVO020000000
VERSION     DAZFVO020000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.289"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(45..1004)
                     /locus_tag="ACHKNB_RS24975"
                     /old_locus_tag="pgaptmp_004995"
     CDS             complement(45..1004)
                     /locus_tag="ACHKNB_RS24975"
                     /old_locus_tag="pgaptmp_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGU4198338.1"
                     /translation="MHSEELKNTLGLAIQIGKLASDQSVLKFFELIDFEKVRDLRDLT
                     QVVNQYGLLKGNISETDFLIASAELESHYSQGVEPVPYGSEYYPLSLVFTPNPPAILY
                     VKGGKHILSELPGVAIVGSRDTSPAGEEITRRITNQIVAAGFIIVSGLAIGTDANAHK
                     AALQAKGKTIAVLAHGLEKAKPKQNSRLAQEILEKGGAWVSEYPIGRPAQKQSFVQRN
                     RIQVGLSVGSILIEAALNSGTMTQAEFANKANRPVFAVIPHLPDNPLNLNCEGTLDLV
                     RNNMARPLKTKRDYENVISIMNESRKKLLGLKYPQKQNTLDLI"
     gene            2584..2873
                     /locus_tag="ACHKNB_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /pseudo
     CDS             2584..2873
                     /locus_tag="ACHKNB_RS24980"
                     /old_locus_tag="pgaptmp_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          902 a          700 c          514 g          875 t
ORIGIN      
        1 aattttgaag atcattgcat ctaaaaaagc ctcttatgag gcctctaaat taaatctaaa
       61 gtattttgtt tttgcgggta ttttagccct aacaatttct tacgagactc attcattata
      121 gaaatcacgt tctcataatc tcgcttagtt tttaacggac gagccatgtt atttcttact
      181 aagtctaatg ttccctcgca atttaaattc aaaggattat caggtaaatg tggtataaca
      241 gcaaaaacag ggcgatttgc tttatttgca aattctgcct gagtcatagt gccgctattc
      301 aatgcagctt caatcagaat tgatcccact gataatccta cctgtattct attcctttga
      361 acaaaagact gcttttgagc cgggcgtcca ataggatatt cagaaaccca tgctccccct
      421 ttctccagta tttcttgagc aaggcggcta ttttgctttg gctttgcttt ttcaagccca
      481 tgagccagaa cagcgatagt tttccctttt gcctgtaatg ccgccttatg agcattcgca
      541 tcagtaccta tagctaatcc actcacaata ataaatccag cagccacaat ttggttagtt
      601 attcgccttg ttatttcttc accggcaggt gatgtatcac gagagccgac aattgctaca
      661 ccgggcaact cagataatat atgcttcccc cccttcacat acaaaattgc aggaggattt
      721 ggtgtaaaga ctaaacttaa gggataatat tctgaaccat atggaactgg ttccacccct
      781 tgagaataat gactctcaag ctctgcgcta gcaatcaaaa aatcagtttc agatatattt
      841 cctttcaaca atccatactg attaaccact tgtgttaaat ctcgcaaatc acgaactttt
      901 tcaaaatcaa taagctcaaa gaatttcaat acactttgat cagatgctaa cttaccaatc
      961 tgaatagcta aaccaagtgt atttttcaat tcttcagagt gcacagcatt actcctcata
     1021 gtttgccgtt tttaataacg acagtggaat tataactcct gcacccgcag attccagtag
     1081 atagaagcaa gcagacaggc ttcctcctgt cgtcttaaca tcatccagta ataacacatt
     1141 tcgcccatgc aggttaccac ctatgactct aatagtagac atatgtccag taattgagcg
     1201 atctccacct tctcgatggg cactcgggac actgcgaaac cgttccagac aacaattcac
     1261 atctccacat cgataaactc gtactatacg ctcagccacc tcaatcaatg ctggtgacac
     1321 tcgcccagca gtatgggaag gtacaacagc aatagaaaag ggatgctcga caaaatactc
     1381 gtgtggtcgt cctggaattc tcaatcctct aactgtttcg atagctaatt gagaaaaacg
     1441 attaatagct cttcgataat tacggtgtcc ttcattctta aaatccatca tgagcctaga
     1501 gatgtcgtca caacgaggat ttcgttcccc taagtactta taaggatgat atacaccgca
     1561 tgaagtcacg cttacgggta gaagcattct ttaatctcat tttgccttgt tattcgttat
     1621 gttaagcgaa acgacatttc aaaaacaaac tgaaaatcaa gaataatctt acaagtcaca
     1681 tttttaacat agcactgcca gcgcgcctct tatccctgac acgcctgcgc gctttatgta
     1741 gtggttttca tgcgactgca tatcataagc aaaagcctgc cattcctgat gggcctcagc
     1801 taaaacgctc ctcaaacgat catgtggatt catgcggtat gacatgtaca atcgccaaag
     1861 agggcatcga gactttttgg aaacatacgt aaccacagtt aatatgtgca tccagtctcg
     1921 aattgcatca catgtctcat caaaaagctt ggtgatggtt gtacggttca actccgcctc
     1981 tttccaataa ttttagctgt caaattctgg atcgccatgc tgaaacgatt ttgactctga
     2041 aaggtattcc ttccggaaac ggtccaaggt tatataaacc tctggtgaat gaacgttttg
     2101 ttcgactcac cacttgtaac aaaagtatta aatgcacctg aaaaaacttt cgccttcaaa
     2161 tggttcgttg aggtcaaaat gatccagcgc agtcctcagc ttaagcacgc tttctctggt
     2221 ttcaaacatc gaattccaaa tgagactata tgctgataac tccttatcaa aatgcgcttt
     2281 cgtgacataa acagaattgt ctaacttagc ctttactctt tcattagttg cactcacttg
     2341 tgagtgaaat tcagcaatat ttttgtttag ccgcgactga tatctctcta aataaacttt
     2401 cccgacccaa ataactatgc cagatgcaat aaaagaccgc cccccaacga agccagaact
     2461 ttaaagacca aatccattgt gcccacctgc aaaaatagta ttccgttata agctaataga
     2521 tttttaatca aacagggaat aattcgcgtc aacatcatcc ctgctctgct ctatcttggc
     2581 gtgaagggga agataaacag aagcatccct atcagtaaag agctttatga agagagaata
     2641 gcgctcgatg ggttcaattt cttcacagac tgctattttc aatctttatc cgtgatggaa
     2701 aaaacgtcta tcgtgttccc tcgaggtcaa ctgacacacg ttctacgcca tacgtttgcg
     2761 gcgtacttaa tgatgttgag tggaaacaac caggccttac aaaaattctc ggaaaccacg
     2821 atataaaaat gactatgcgt tatgagcatc tggtaccgaa tcatctggaa acatagctcc
     2881 gtttcaatcc actggcaacg ttaccaaatg gcgacaatgt ggcattaccc cgtaataacc
     2941 accactaacc accacattaa gttatcgttt ttaaaaaact cattgttttt a
//
LOCUS       DAZFVO020000288         2991 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFVO020000288 DAZFVO020000000
VERSION     DAZFVO020000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.290"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..878
                     /locus_tag="ACHKNB_RS24985"
                     /old_locus_tag="pgaptmp_004997"
     CDS             <1..878
                     /locus_tag="ACHKNB_RS24985"
                     /old_locus_tag="pgaptmp_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HGU4198339.1"
                     /translation="LHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGN
                     AAALINSPRRMAESLAGMFAVLTGLPGDPSLSLTGKAATPSGSLTTNRDSMTEQAMPQ
                     LYRTLSSLRYTLSGQDDPQILIGLTPAAQKNIRLLRTVMQSATLVSQAQTVGKLLDQV
                     VRQSATHVQDTDREGGLAWLESSADVQRINRDLSDALEQQVLDLSAQGHTGTALALRD
                     ARLALTEDLTTRSVHLPGASLVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVF
                     VPGGRSVEVISEQQD"
     gene            862..1986
                     /locus_tag="ACHKNB_RS24990"
                     /old_locus_tag="pgaptmp_004998"
     CDS             862..1986
                     /locus_tag="ACHKNB_RS24990"
                     /old_locus_tag="pgaptmp_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HGU4198340.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISINGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGNQQTDRLVLGENLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGIISDEQIGRYRPKIILADQQTDTTGARQRALREMRRAVARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            1976..2590
                     /locus_tag="ACHKNB_RS24995"
                     /old_locus_tag="pgaptmp_004999"
     CDS             1976..2590
                     /locus_tag="ACHKNB_RS24995"
                     /old_locus_tag="pgaptmp_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HGU4198341.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEATFTGNVTVDKNLHIKGNL
                     TIDGTGKSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGAPENG"
     gene            2583..>2991
                     /locus_tag="ACHKNB_RS25000"
                     /old_locus_tag="pgaptmp_005000"
     CDS             2583..>2991
                     /locus_tag="ACHKNB_RS25000"
                     /old_locus_tag="pgaptmp_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HGU4198342.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLVSVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPM"
BASE COUNT          618 a          803 c          946 g          624 t
ORIGIN      
        1 gcctgcatga tgtgcaggcc atgaccgaca ccatcagcga taaaattgat gcgctggaga
       61 atgccgtttc gggaatgggg attgtgcagg atatcagtgc ctttacggcc agtttcactg
      121 ccctgaaagg gaatgctgcc gccctgatta attcgccccg tcgcatggct gaatctctgg
      181 ccggaatgtt tgcggtgctg acgggactgc ccggcgatcc ttccctgtca ctgaccggga
      241 aggccgcaac gccgtccggc agcctgacca caaatcgtga cagcatgaca gagcaggcaa
      301 tgccgcaact gtaccgcaca ctttcttccc tgcgttatac cctcagcggg caggatgatc
      361 cgcagatcct gattggtctg acgcctgcgg cacagaaaaa tatccgtctg ctgcggacag
      421 tcatgcagag cgcaaccctg gtgtcacagg cgcagacggt aggaaaactg ctggatcagg
      481 tcgtccggca gagtgcgacg cacgttcagg atacagaccg ggagggcgga ctggcctggc
      541 tggaaagttc tgccgatgtg cagcggatca accgggattt gagtgatgcc ctggaacaac
      601 aggtgctgga tttgtccgca cagggccata ccggaaccgc ccttgcatta cgtgatgcca
      661 ggcttgcgct gacagaggat ttaacaacgc gtagcgtgca tcttcccggt gcttctctgg
      721 tcatggtgag aaccaccgaa ccggcactgg tcacgctgta ccgtgccacc ggcaacagcc
      781 gtcgctggca gtattttgtc cgcaggaaca acatccctga tccggttttt gttcctggtg
      841 gtcgcagtgt ggaggtgatt agtgagcagc aggattgagc tgtatattgg cggcagtatt
      901 ttttccggct ggctgacggt aagcgtccgt cgttctctgg agcatctggc cgggtcgttt
      961 gaactggggc tgatgctgcc cggtgagcgc atcccctcag ccctgcggac cggtcagtcc
     1021 ctgacgctca gaattaacgg gcagactgtc atcagtggct ggctggatca ggtcagtcag
     1081 cggatcagtg caacccgtca tcagatcagc attaacggtc gggataaaac cggcgacctg
     1141 gtggactgtg ccgccatcca tcccggcagc cagtggcgaa accggacgct ggcacagatt
     1201 gccgcagatt tatgtgcgcc cttcggaata gccgtgcgct ggcaggttaa cgacgacacg
     1261 gctgcacgcc ccttcagctc ttttaccctg gaaaactcag aaaccgtggc ggatgcgctg
     1321 acccgtgcgg cccggcatcg tggggtgctg gtgaccagta atgctgacgg tgatctggtg
     1381 ttcacccagg caggaaatca gcagacggac agactggtgc tgggggaaaa cctgcttgat
     1441 gccgattaca acacggactg gcgaggacga tacagcgaat accgtgtccg ggggcatgga
     1501 cgcggtggtg gcaaacgggg agacagcgaa tctgccgccc ggctggcggc cccagtgggc
     1561 atcatcagtg atgagcagat cggtcgctac cggccgaaaa tcatcctcgc cgatcagcag
     1621 acagacacca ccggtgcacg gcagcgcgcc ctgcgggaga tgcgccgtgc ggttgcccgt
     1681 tcagaacggt tttccgccac cgtgcgtggc tggttccggg atgatggccg gttatgggat
     1741 gtcaatctgc tgaccggtgt ttcagccctg cgtttcggta tcgaacagac tgaactgctg
     1801 gtctgccagg tggagttttt actggatgag cagaacgggg aagtcacccg gctggtactg
     1861 gcaccgcgtg acggctttat cgttccggca gagccggaca gtaagggccg tggtggttcc
     1921 ggtgatgatg ttgatgcctt tattcgccag cagatgaaaa aacaggggat tagctttgat
     1981 gaatgatgaa gtgttcagcc gcctgattgc cccggtaacg cgcggcattc gcctgctgtt
     2041 tggccggggc gttctgaccg gcacacatga cgaactgaaa atgcagaatg tgcagctcac
     2101 cggcatggac ggcgaaacct ttgatgatgt ggagcgtccc cagcagtacg ggcagatcag
     2161 tgttcccctg ccgggtgcgg aaaccttttt tgcctgtctg ggcgggcagc gtgatcagac
     2221 cgtggtgctt gtggtggaag accgccgcag tcgcccgacc ggactcactg ccggagatac
     2281 cggcgtgtat caccatgagg ggcaccgtat acgtctgaca aagaacggtc ggatcattgt
     2341 gacctgtaag acgctggaaa tttacgccga tgaaggtgtt caggtggata caccggaagc
     2401 cacctttacg ggcaatgtca cggtggataa aaacctgcat atcaaaggta atctgaccat
     2461 tgacggcaca ggaaaatcac agggtacgtt cacgatgtcc gaggctgtta ttgcgggaat
     2521 cacctattcc ggccatgtac atcatgataa cggtgaaggc agtaagacag gagcgccgga
     2581 gaatggctga tattgcaatt gtgtgggatc agggatgcgg ttcgctgcaa ctgaacggtg
     2641 cagacctgct gacagatgac agcctgctga cagcggttct gatttcactg tttactgacc
     2701 gccgggcact ggcatcggat gaaatccctg atggcacacg tgaccgccgg ggatggtggg
     2761 gagacagttt tcgcccgcgc cccattggct cccgtctgtg gcttctcagc cgggaaaaaa
     2821 cgctggtctc cgtgataagc cgtgcccgtg cctacgcgga tgaggcgctg ggctggctca
     2881 gtcaggatgg cgtggcgtca tccgtggtct gtcatgcaga acgtgtggga cacgcgcggc
     2941 tggcgctttc ggtgcgcata accctgcctg atggttcggt aagacccatg a
//
LOCUS       DAZFVO020000289         2967 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFVO020000289 DAZFVO020000000
VERSION     DAZFVO020000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.291"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(932..1132)
                     /locus_tag="ACHKNB_RS25005"
                     /old_locus_tag="pgaptmp_005001"
     CDS             complement(932..1132)
                     /locus_tag="ACHKNB_RS25005"
                     /old_locus_tag="pgaptmp_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020323376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198343.1"
                     /translation="MEYGKLAAQIGMRLKLEVMESARGYYIGTQTEYGEPVSRESNEY
                     FDTEQQAEAALAGRLWTQKQNP"
     gene            complement(1175..1507)
                     /locus_tag="ACHKNB_RS25010"
                     /old_locus_tag="pgaptmp_005002"
     CDS             complement(1175..1507)
                     /locus_tag="ACHKNB_RS25010"
                     /old_locus_tag="pgaptmp_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198344.1"
                     /translation="MDYQTRLNSDITKEIDYLASLRKQRMVADLRTELVYGTLVRLAD
                     MICNTVTDWSLPCPVLPLSSVQQWHKAREIVLADYEDFGHAAWDYARHHMKTELSFGY
                     ACYKNDIA"
     gene            complement(1717..2001)
                     /gene="ccgC"
                     /locus_tag="ACHKNB_RS25015"
                     /old_locus_tag="pgaptmp_005003"
     CDS             complement(1717..2001)
                     /gene="ccgC"
                     /locus_tag="ACHKNB_RS25015"
                     /old_locus_tag="pgaptmp_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CcgC"
                     /protein_id="HGU4198345.1"
                     /translation="MYHYLVFVPLALIISAAALALPWFIVRAGYRELVQITCACVSVL
                     GLCAATGVFSYFDNKPLAFLYGCGALACFLYAVDCAIPLYMTRKKPRKQL"
     gene            complement(2153..2950)
                     /locus_tag="ACHKNB_RS25020"
                     /old_locus_tag="pgaptmp_005004"
     CDS             complement(2153..2950)
                     /locus_tag="ACHKNB_RS25020"
                     /old_locus_tag="pgaptmp_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HGU4198346.1"
                     /translation="MNTQLLKVGQCLKHLNDDVAVSEVTLSDGEISYTLLNLELPTAF
                     TVEQARISARFGWTLTDKTLSLDEIRSRREVVRVCKQRRDEEAARKAEEEQRSREQVS
                     TNPEYQDLLTKKDEPSTTKLAAKNIRLLLKKHFKGIKFSVRMRDYSCINVGWTDGPTT
                     DEVDAVISRFQEGRFDGMTDMYEYGNDPFNKVYGGVQYLFTSRECSDALIEEAIASVK
                     TRYKSVVGDDCTVAAFRSGKLWNVGNEYFSHGLQSAIHQAAAAISKK"
BASE COUNT          649 a          792 c          855 g          671 t
ORIGIN      
        1 ttagcggccg ccatgcggcc ggttacgggg gacaccgcac cgtcacggcc agcgccccgc
       61 tgagctgcgc aatccacaga taacacaata gcgcactggc aaaggatgcc gacgcctgaa
      121 gggcgttggc accccgaagg ggcggggcgg ccgcttgcgg ccgggcgagt ccggcgcagg
      181 gtgtggcttg ccaagcggag cgcggaggcc gtaggccgga ggcgttagcg gccgctgccc
      241 gcgtaagcgg ggcgagacgg gaaccggctc gatgcgcagc acagcagagc ggccccgaag
      301 gggtaacgcc cggtgtggca tcaggattta gcgcaatggc agaacatgag ctggagagat
      361 caccggcaag cagcagcaaa ggggcggcgc agccgccccg atggctgtta cttgtctttg
      421 tcgcgtagcg ctttgattag gccggttacg gccgtaatca gagcggccag cgaggtgagg
      481 atttgcggga ggttttcgag gatggtagag gtcatgtagc acctgtagag aagttggcgg
      541 ggtgtcgttt ccgacggcag cattgtaacc gggcggataa ggcaggtttc actgcggtca
      601 taggcggaac gacccacgcc aacggaacgg cttcatgacc gggcagctga gataccggcg
      661 aacctggctg gcgctgacgc cagtcgccaa gcaccagcgc ggagggcaaa gcccggaggc
      721 caagcggagc gcggaggccg caggccggag gcgttagcgg ccgctgcccg cgtaagcggg
      781 gcgagacggg aaccggctcg atgcgtagca cagcagagcg gccccgaagg ggtaacgccc
      841 ggagtctgcc gctgtttctc cctcgttcta tctgaaatcg gcggtatggc cataaaagaa
      901 taatggcgcc cgtttgggcg ccattgtggg atcaggggtt ttgtttctgt gtccataatc
      961 tgccagccag tgcggcttct gcctgctgct cggtatcaaa atattcgtta gattcgcgtg
     1021 ataccggctc accatattct gtctgcgttc caatgtaata gccgcgggct gactccatca
     1081 cttccagttt caggcgcatc ccgatttgag cggccagttt tccatattcc atcttctctc
     1141 cttcaggggg cgttagcccc catgttgtta atcatcaggc aatatcgttt ttgtagcagg
     1201 cataaccgaa gctaagctct gttttcatat ggtggcgggc atagtcccag gcagcgtggc
     1261 cgaagtcctc ataatctgcc aggacgattt cgcgggcttt gtgccattgc tgtacggaag
     1321 aaagcggcag aacagggcag ggaagtgacc agtcagtgac ggtgttgcag atcatatctg
     1381 ccagacgcac cagtgtgccg taaaccagct ctgtgcgcag atccgccacc atgcgctgtt
     1441 tacgcagtga tgccagataa tcaatctctt tggttatgtc agaatttaag cgggtctggt
     1501 aatccatgat gtactccttt gcgcgccgat accggcaatt ttgcgggcgg cggtgttgcc
     1561 tcccgatgat ttaattatcg gtgattatgc cctcaaagtc aatacaagta cggaatatac
     1621 atacaagatt ttatatcttg caaagcgtcc atagagcgcc tgaatcgttt tctgacagcc
     1681 tcaataaaaa aaggcgggga ttacccgcct tttttcttac agctgcttac gtggcttttt
     1741 acgcgtcata tacaacggta tcgcgcagtc taccgcgtac aaaaagcacg ccagcgcgcc
     1801 gcagccgtac aggaacgcaa gcggcttatt atcgaagtag ctgaaaaccc ctgtcgccgc
     1861 acacaggccc agaacagaga cgcaggcgca ggtgatctgc accagttccc tgtatcccgc
     1921 acgaacgata aaccagggca gggcaagcgc agcggcgcta ataattaatg cgagagggac
     1981 aaaaacgaga tagtgataca tgtgaactcc ttgatggttg ccgataccgg cgattgttcg
     2041 ggcggcggta ttgcctcccg atgatttaat tatcggtgat cacgccctta aagtcaatac
     2101 aagtacggaa tatatatact ataatttacc ccccgttagg ggggcatcct gtttattttt
     2161 tactgattgc ggccgctgcc tggtgaatgg ctgattgcag accgtggcta aaatattcat
     2221 tacctacgtt ccacagcttg ccggaacgga aagccgcgac cgtgcaatca tccccaacta
     2281 ccgatttata gcgggtttta acgctggcga tcgcttcctc gatcagagca tctgagcatt
     2341 cgcgcgaggt gaaaagatac tgcacgcccc cataaacctt gttgaatggg tcgttaccgt
     2401 attcatacat atccgtcatg ccgtcaaaac ggccttcctg aaaacggctg attacggcgt
     2461 ccacttcatc agtcgtcggg ccgtctgtcc agcccacatt gatgcaggaa taatcacgca
     2521 tacgcactga aaacttaatg cccttgaaat gctttttcag cagcaggcgg atatttttag
     2581 ccgccagttt cgtcgtgctg ggttcgtctt ttttggtcag tagatcctga tactctggat
     2641 tcgttgatac ctgctcacgg ctgcgctgtt cttcctctgc cttccgcgcg gcttcttcat
     2701 cgcggcgctg cttacacacg cgaacgactt cgcggcggct gcggatttca tcaagggata
     2761 gcgtcttatc tgtaagcgtc cagccaaagc gcgcgctgat acgggcttgc tcaacggtga
     2821 acgcggtcgg taattcgagg tttaagaggg tgtaagagat ctcgccatct gagagggtca
     2881 cttcactaac tgctacatcg tcattgaggt gcttcaggca ctggcctact ttcagtaatt
     2941 gagtattcat gtacgactcc tgtttgc
//
LOCUS       DAZFVO020000290         2899 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFVO020000290 DAZFVO020000000
VERSION     DAZFVO020000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.292"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..288
                     /locus_tag="ACHKNB_RS25025"
                     /old_locus_tag="pgaptmp_005005"
     CDS             <1..288
                     /locus_tag="ACHKNB_RS25025"
                     /old_locus_tag="pgaptmp_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4198347.1"
                     /translation="VALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRAD
                     RERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            269..1975
                     /locus_tag="ACHKNB_RS25030"
                     /old_locus_tag="pgaptmp_005006"
     CDS             269..1975
                     /locus_tag="ACHKNB_RS25030"
                     /old_locus_tag="pgaptmp_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4198348.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            1975..>2899
                     /locus_tag="ACHKNB_RS25035"
                     /old_locus_tag="pgaptmp_005007"
     CDS             1975..>2899
                     /locus_tag="ACHKNB_RS25035"
                     /old_locus_tag="pgaptmp_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HGU4198349.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREA"
BASE COUNT          774 a          670 c          794 g          661 t
ORIGIN      
        1 gttgctcttt acgacaaaat actcaaagct gaacaggccc ttgaccggaa tattgcccgt
       61 atcgagtcaa ttgaacgctc attgctgacg ctggacgtcc tggctgagac agcaccaaaa
      121 cttcgtgctg accgggaaag aatcaacgcc gccagagata aactcagagc ggaaaccgat
      181 attctgacca gccagcgccg gggtgttatt acgccagtca gtgacatcgt gtcatcgctg
      241 catgaaatga gtaattcggg gagactggat gacattccgg aagaataaaa cgcgatgtga
      301 tgaacctgca gaaatgactg agaccgaaca acgtctgttc attatgacaa aactgagcaa
      361 tccctggtgg cgcctcaatc atctctacaa aatacagaac gaaaaaggtg aactggtcac
      421 cttcagaatg cgaccggcgc aacgtcagtt gtttcgtaac atgcataata aaaacattat
      481 cctgaaagcg cgccagctgg gattttccac agccattgat atttatcttc tcgaccaggc
      541 attattcatt ccgcatctca aatgcgggat cgtcgctcag gataaacagg ctgccagtga
      601 aattttccgc acaaaaattg ctgtaccgtt tgatcatctc cctgactggc tgagagcctc
      661 attcaccatc gttgaacgtc gtagcggtgc cagcggtggc tatatcctgt ttggtcacgg
      721 ctcgagtatt caggtggcaa cctcatttcg ctcaggtacg gtgcagcgcc tgcatatctc
      781 agagcacggc aaaatttgcg cgaaatatcc ggctaaggcg aaagagctgc gaaccggtac
      841 gcttaatgcc gtctctgatg aatgcattat ttttgatgaa tccacggctg aaggcgtggg
      901 tggtgatttt tacgagatga gtaaccgtgc acaggagagc actgcatcag gcttattgct
      961 gacggcacag gattataaat tccattttta cgcatggtgg caggatccta aatacagcgc
     1021 cagagtgcct gaaagcgggc tgaagctgtc acgggaaaaa atgacgtatt tttctgcggt
     1081 tgagaaggca atgaacatca cgcttaccga tgagcagaag cagtggtaca tcagtaagga
     1141 aactgaacag cgtgaggaaa tgaagcagga gtttccctca acgccacagg aggcgtttct
     1201 gacgtccgga cgacgtgtgt tcagtgccga aagtacattg caggcagaat cattctgttc
     1261 gccaccgatg attgtttatg acattgaacc tgttacagga gcgaagacta aagctcagtc
     1321 tctgcgtgaa ggaaataaaa acgagttgca gcggacgctg atgaattatc tgctggtatg
     1381 ggaactgccg gatccggatg aagagtatgt ttgtggggca gatactgccg aagggctgga
     1441 gcacggagac cgctcatcgc tggatgttgt caaacgcagt aatggcgagc aggtggctca
     1501 ctggttcggg catctcgatg ctgaactttt tgctcatctc atttcgcagg tctgtcgtat
     1561 gtataacaac gcgtttgtgg ggccggagcg taataatcac ggacatgcag ttatcctgaa
     1621 actccgggaa ctctatccga cacgttatat ctacaacgaa cagcatcttg accaggcata
     1681 tgacgacgat acgccccgcc ttggctggct gacaacccgt cagagcaaac ctgttctgac
     1741 cgaaggaatg aaaacgctcc tgaataatgg aatatcaggg atccgctggt caggcacatt
     1801 atcggaaatg aacacctacg tttatgacgc gaaaggctcc atgaatgcac aggaaggctg
     1861 ctttgatgat cagctcatga gctacatgat tgcccaggag atgcgcgcca gaatgccggt
     1921 gagggtaaaa cagaaaacgg ataaacgcag aaccacacac tggatggcac actgatgaaa
     1981 aatgaaatta acaccacagc gatgaaaaac gatcatggat ccacgccgcg tttttctcag
     2041 cgtcagttac tgtctctctg ttctgatatt gacagtcagc ctctctggcg tgatgccgca
     2101 aacaaggcct gtgcgtatta tgatggcgac cagctggcac cggaagttat ccaggtactg
     2161 aaagatcgcg gtcagcccat gaccatccat aacctcattg cccccacggt agatggtgta
     2221 ctgggaatgg aggcaaaaac aagaacggac ctgatagtga tgtcagacga tccgaacgat
     2281 gaaacagaga aactggcaga ggccattaat gcggagtttg ctgatgcgtg ccgtcttggc
     2341 aacatgaata aggcccgctc cgatgcctat gcggaacaaa tcaaggcggg gctcagttgg
     2401 gtggaggtca ggcgcaacag cgatccgttc ggacctgaat tcagggtatc cacagtcagc
     2461 agaaatgagg ttttctggga ctggctcagc cgggaatccg acctgagtga ctgccggtgg
     2521 ctgatgcgtc gccgctggat ggataccgat gaggcaaaag ccacgttccc gggaatggct
     2581 caggttatcg attatgccat tgatgactgg cgtggttttg tcgataccac gattactgaa
     2641 ggccagccca gtccgttgat gagtgcatgg gaagagtatc agtcatggga tcgcagggag
     2701 aatgaatggc ttcagcgtga acgccgtcgt gtgctgcttc aggtggttta ttaccgtaca
     2761 ttcgagcgtc ttccggtgat tgaactcagt aatggacggg tggtggcttt tgataaaaat
     2821 aatctgatgc aggcggtggc tgtggcatcc gggcgggttc aggtgaaagt cgggcgggta
     2881 agtcgtattc gtgaagcct
//
LOCUS       DAZFVO020000291         2887 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFVO020000291 DAZFVO020000000
VERSION     DAZFVO020000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.293"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            143..1243
                     /locus_tag="ACHKNB_RS25040"
                     /old_locus_tag="pgaptmp_005008"
     CDS             143..1243
                     /locus_tag="ACHKNB_RS25040"
                     /old_locus_tag="pgaptmp_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HGU4198350.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1268..>2887
                     /gene="torZ"
                     /locus_tag="ACHKNB_RS25045"
                     /old_locus_tag="pgaptmp_005009"
     CDS             1268..>2887
                     /gene="torZ"
                     /locus_tag="ACHKNB_RS25045"
                     /old_locus_tag="pgaptmp_005009"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HGU4198351.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARN"
BASE COUNT          791 a          646 c          777 g          673 t
ORIGIN      
        1 caattaatga ttacattgta ataaatcata ttctttatcg attatttcag gcagtgttgt
       61 gccgagttat gcaagcggtt aattcgttgt gtatttaatt atacaatgat ttcggtgttc
      121 tggaatttat ttagaggaat ctatgcgagg gaaaaaacgc attgggttat tgtttttgct
      181 gatagcggtt gtggttggtg gcggcgggtt attgctggcg caaaaagcct tacataaaac
      241 gtcggataca gcattttgcc tttcctgcca ctcgatgagt aaaccttttg aggaatatca
      301 gggaactgtc cacttttcga accagaaagg gatacgtgcg gaatgtgcag attgccatat
      361 tccaaagtca gggatggatt atttatttgc taaattaaaa gcatctaaag atatttatca
      421 tgaatttgtt agcggcaaaa tagacagtga cgataagttc gaaactcatc gccaggaaat
      481 ggccgaaaca gtatggaaag aattaaaagc aactgactct gcaacgtgcc gtagttgcca
      541 ttcttttgat gccatggata ttgcctcgca aagtgaatct gcgcagaaaa tgcataacaa
      601 agcacaaaag ggcggcgaaa cctgtatcga ttgtcataaa ggcattgccc attttccgcc
      661 agaaataaaa atggatgaca acgcggcgca tgagctggaa agtcaggccg ctacttcagt
      721 gactaatggc gcacatattt atcctttcaa aacttctcgc ataggcgagc tggctaccgt
      781 gaatcctggt accgatctca ccgtcgttga tgccagtggc aaacagccga tcgttctgtt
      841 gcagggttat caaatgcagg gcagtgaaaa cacgctctac ctggcggcag gtcaacggct
      901 ggcgctagcc acattaagtg aagaaggtat caaggcgctc acggtaaacg gggaatggca
      961 ggctgacgaa tacggcaatc aatggcgtca ggcgtcttta cagggtgcgc ttaccgatcc
     1021 cgcattagcg gaccgtaaac cgctatggca atacgctgaa aaacttgacg atacctattg
     1081 cgctggttgc catgcgccta ttgccgctga ccattacacc gtcaatgcat ggccgtccat
     1141 tgccaaagga atgggtgcgc gaaccagcat gagcgaaaac gaactggaca ttttaacgcg
     1201 gtatttccag tacaacgcca aagatattac cgagaaacag tgatcctcgt tgatcaggga
     1261 ggaagttatg acattaacaa gacgtgaatt tattaaacac agcggtattg ccgctggagc
     1321 gctggtggtg acatcggcag caccgctccc cgcatgggca gaagagaagg gcggtaaaat
     1381 cctcaccgcg ggacgttggg gagcgatgaa cgtagaagtg aaggacggca agattgtttc
     1441 ttcaacaggc gcgctggcga aaaccatacc gaactcctta cagtctacgg cggcggatca
     1501 ggtacacacc acggcacgta ttcagcatcc gatggtaaga aaaagctatc tcgataatcc
     1561 actgcaaccg gcgaaaggtc gtggcgaaga tacttatgta caggtgagct gggagcaagc
     1621 attaaaactg attcatgagc aacacgatcg cattcgcaaa gctaacggac catctgctat
     1681 ttttgccgga tcatacggct ggcgctcttc tggcgtgttg cataaagcgc aaaccttact
     1741 gcaacgttat atgaacctgg cgggcggtta ttccgggcat agcggcgatt attcaaccgg
     1801 ggcagcacag gtgatcatgc cgcatgtggt cggttctgta gaggtgtatg aacagcagac
     1861 ctcctggccg ctgattctgg aaaacagcca ggctgtggtg ctgtggggga tgaacccgct
     1921 gaatacctta aaaattgcct ggagcagtac cgatgagcaa gggctggaat acttccatca
     1981 gctgaaaaaa tctggcaaac cagtgattgc cattgatcct atccgctccg aaaccatcga
     2041 gttctttggt gataatgcta cctggatcgc gccgaatatg ggcaccgacg tggcactgat
     2101 gttagggatt gcgcataccc tgatgacaca aggtaaacac gataaagtgt tccttgaaaa
     2161 atacaccacc gggtatccgc agtttgaaga gtatctgaca ggtaagagcg ataatactcc
     2221 gaagagcgcg gcctgggcgg cggaaattac tggcgtaccg gaagcgcaga tagtcaaact
     2281 cgctgaactg atggcggcga atcgcacaat gctgatggct ggctggggaa ttcagcgcca
     2341 gcaatatggt gaacagaaac actggatgct ggtgacgctg gcggcgatgt tagggcagat
     2401 tggtacaccg ggcggcggtt ttggtttctc ttatcattac tccaacggtg gcaatccgac
     2461 gcgtgttggc ggcgtgctgc cggaaatgtc tgcggcgatt gccggacagg ccagcgaggc
     2521 cgctgatgat ggcggaatga cggcgattcc tgttgcccgt atcgtcgatg cgctggaaaa
     2581 tcctggtggc aaatatcagc ataacggtaa agaacaaacg tatccgaata tcaaaatgat
     2641 ctggtgggcg ggtggcggta actttaccca tcaccaggat actaatcgtc tgatcaaagc
     2701 atggcagaaa ccggagatga tcgtcgtttc tgaatgctac tggaccgcag ctgctaaaca
     2761 tgcagatatc gtattaccga tcaccacatc gtttgagcgc aatgacttga cgatgaccgg
     2821 tgattacagc aaccagcata ttgtgccgat gaagcaggct gttgctccgc aatttgaagc
     2881 gcgtaac
//
LOCUS       DAZFVO020000292         2874 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFVO020000292 DAZFVO020000000
VERSION     DAZFVO020000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.294"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..699
                     /locus_tag="ACHKNB_RS25050"
                     /old_locus_tag="pgaptmp_005010"
     CDS             <1..699
                     /locus_tag="ACHKNB_RS25050"
                     /old_locus_tag="pgaptmp_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198352.1"
                     /translation="VLWMAHTGTFGEVQAFGAKRNDFLATCTFIPKAPEPDYLWAAAV
                     CATCAPSLGADPARPLQTLALPSRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQ
                     IERQVTMYRQNVYGESDPSYLDVETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRA
                     GQNIVTPEIMKLQLISLGEEWVELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLV
                     NQFRFLAAQIRFIL"
     gene            708..1073
                     /locus_tag="ACHKNB_RS25055"
                     /old_locus_tag="pgaptmp_005011"
     CDS             708..1073
                     /locus_tag="ACHKNB_RS25055"
                     /old_locus_tag="pgaptmp_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGU4198353.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASATSTRVQ"
     gene            1088..1564
                     /locus_tag="ACHKNB_RS25060"
                     /old_locus_tag="pgaptmp_005012"
     CDS             1088..1564
                     /locus_tag="ACHKNB_RS25060"
                     /old_locus_tag="pgaptmp_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HGU4198354.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            1691..>2874
                     /locus_tag="ACHKNB_RS25065"
                     /old_locus_tag="pgaptmp_005013"
     CDS             1691..>2874
                     /locus_tag="ACHKNB_RS25065"
                     /old_locus_tag="pgaptmp_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4198355.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATR"
BASE COUNT          654 a          713 c          900 g          607 t
ORIGIN      
        1 gtgctgtgga tggcgcatac cggcacgttt ggtgaggtgc aggcattcgg ggcaaaacgc
       61 aatgattttc tggcgacctg cacattcatt cccaaagccc ctgaaccgga ttacctctgg
      121 gcagcggcgg tctgcgccac ctgtgcgccg tctctggggg cagatcctgc gcgtccgctt
      181 cagacgctgg cgcttccgtc acgcatggcc ccggccccgg ccgatcgtct gacgcgtgaa
      241 gagcgtaaca gtctgctgtg gggagggatt gccacggtca gtgtggctgc cggtgatgtg
      301 gttcagatcg aacgtcaggt gaccatgtat cgccagaacg tgtacggcga aagtgatccc
      361 agctatctgg atgtggaaac catctacacc ctctcctggc tgcgttattc cctgcgtact
      421 tttatcacac aacgttttcc gcgccataaa ctggctgatg acgggacgcc cgttcgtgcc
      481 ggtcagaata tcgtgacgcc ggagatcatg aagcttcagt taatttcgct gggtgaagag
      541 tgggtggaac tcgggctggt cgaaaatctg gacaccttta aaaagaatct gcttgtggag
      601 cgtaatgcgt cagatcgaaa ccgtcttgac gtgttatgca cgccggatct ggttaaccag
      661 ttccgcttcc tggcagcaca gatccgtttc attttgtgag gtaaggcatg agcggaaaac
      721 agtatcaggg cacggccact atccgtgtga acggtcagga atacgagacg cttgaggggg
      781 ctacattttc tccgtcaggt tttgaacgtg aagtggtgaa aggcgcgaaa gtctatggtt
      841 accgccagaa accacgtgaa gcgacactgg actgcaaatt tccggcaggc ggagaaggtt
      901 caccagccgc cgatgaaatc aacaactgga ctgctgtcac tatcgagttt gtggcggata
      961 ccggcgaagt ccacatgatg acgaaggcct ggagcagtga accggcctcg cttgacggtg
     1021 gcggggagat ctccgtgaag ttcgccagcg ccaccagtac ccgtgttcag tgatcaggaa
     1081 aaataaaatg accacacgta agaaaaaaac ggcagtttcc gaggcagccg tgatggaggc
     1141 aatccgggaa gcacttgagg gggctgatcc acgcactgcc gggctgacag agcaactggc
     1201 aaagggatat gtggatctgc ttgatggtct gccgtttggt gagacccgtg aatatcgtgt
     1261 cacgttccgg gaactgacag cgaaagacag tattgacgca gaagcagaag ccgagcgcgt
     1321 ggtggagaca aacaatggcc cgatgctgat agcgtctccg tcgttacgcg gtgttgcact
     1381 gctgcgccgt cagattgccg cagtgggaga cattgaaggt ccgttgtcac cccgtcagat
     1441 tggacagtta agcgagcgcg atctctcccg cctgatggca gcggtcagcc tgctggatac
     1501 cgcgcttgcc ggaaagctgg cagctgaccg ggggcgatca ggcgcagtgt cgggatcaga
     1561 ttgaagaagc ggcaattatc ctggggatgg tgacaaaaag tggcccggaa tgggcgctta
     1621 acctccccct gtcgcaactt taccggcact gccgacagac agaaaaaatc atcagaacga
     1681 agcagtaagt atggcccgaa atctcagagc atctctgata gttgatttgc tcggcaatat
     1741 ctcagccaaa tcccgccagt ggtctcagga gctgggggca ttctcacgct ccggccaggc
     1801 ggggctgggt ggtctgggaa atgcggcccg ccgtgccggg caggaaacag atgtactcgg
     1861 aagcaggatg cagcgcacgc tggccggggt gcggggcagt atccgccatg tcacttctga
     1921 ttttgaccgt cttcagggca gtattaccgg caccatcggg cggatcagca atctttacgg
     1981 aatgctggcc ggtggtgcag ctgtatatgg ctttaaccgg ggatttattc gcccggcagc
     2041 ggagatggaa acctatatga ttcgcctgaa ctccctttac aaaggggatc gggcgaaaac
     2101 tgatgatgtc cgcaggtggg caatacagaa tgcgaaggaa accacctggg ggctggctgg
     2161 cgtcatgcag gaatatacgt ccagtctggg ctttggtatg agtgacaggg aggcccgaaa
     2221 ttttgtcacc atgcttcagg atcagggtgc cgtgggcggc tggtcactgt ccgatgcaca
     2281 gggggcatcc ctgcaactga aacagatgta tgcaagaggc agtattcagg cagcggatgc
     2341 aaatattctt gccacctacg gcattaatgc ctaccgcgtg ctggctgatc gtctgggtgt
     2401 ggaccagaaa gtggtcagaa aacttggtga aaaagggctg cttgggccgg acagtattcg
     2461 cctgttattt cagacgctgg cggaacaggc caggggagca caaaaggacg caatgaattc
     2521 ctggagtggc ctgacggcca tgatgggtga cgtctgggat cagtttgccc gggatgtgat
     2581 ggacagtggg ccatttgaaa aactgaaggg taatctcagg ggatttcttt catgggttga
     2641 cagtgccaaa tctgatggca gttaccattc cctggcggaa agcacggcat cagccatgaa
     2701 ccagggcttt gagtatgccc gtgatgcagt gacagggttt tatcaggcga tcagaaaggt
     2761 acgcgacacg cttcaggctc tgcgggatgc gggctatggt gatgcgctgg atcgcattgg
     2821 tcagggggca cagaccgccg cgaaatacct gctgtacatg tatctcgcca cccg
//
LOCUS       DAZFVO020000293         2861 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFVO020000293 DAZFVO020000000
VERSION     DAZFVO020000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.295"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..455)
                     /locus_tag="ACHKNB_RS25070"
                     /old_locus_tag="pgaptmp_005014"
     CDS             complement(<1..455)
                     /locus_tag="ACHKNB_RS25070"
                     /old_locus_tag="pgaptmp_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HGU4198356.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTFVLLAATGKTSCRDMSPGEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERT"
     gene            complement(452..748)
                     /locus_tag="ACHKNB_RS25075"
                     /old_locus_tag="pgaptmp_005015"
     CDS             complement(452..748)
                     /locus_tag="ACHKNB_RS25075"
                     /old_locus_tag="pgaptmp_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198357.1"
                     /translation="MDSSSQLFWCLYITSFFGAFVITRWLCRKVISIFHKKYLIGLAA
                     TSLINALARYHKGEYYSRIKTDDGFNIFVIPPEYRVQLDRESNIYHISRSVDTA"
     gene            complement(738..935)
                     /locus_tag="ACHKNB_RS25080"
                     /old_locus_tag="pgaptmp_005016"
     CDS             complement(738..935)
                     /locus_tag="ACHKNB_RS25080"
                     /old_locus_tag="pgaptmp_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198358.1"
                     /translation="MNSLPAGWARPLMAKKHHFFKTGDNISICRRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            complement(928..1260)
                     /locus_tag="ACHKNB_RS25085"
                     /old_locus_tag="pgaptmp_005017"
     CDS             complement(928..1260)
                     /locus_tag="ACHKNB_RS25085"
                     /old_locus_tag="pgaptmp_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198359.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIQMILS
                     SMEDKANE"
     gene            complement(1299..1484)
                     /locus_tag="ACHKNB_RS25090"
                     /old_locus_tag="pgaptmp_005018"
     CDS             complement(1299..1484)
                     /locus_tag="ACHKNB_RS25090"
                     /old_locus_tag="pgaptmp_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198360.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            complement(1481..2032)
                     /locus_tag="ACHKNB_RS25095"
                     /old_locus_tag="pgaptmp_005019"
     CDS             complement(1481..2032)
                     /locus_tag="ACHKNB_RS25095"
                     /old_locus_tag="pgaptmp_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4198361.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            complement(2036..2551)
                     /locus_tag="ACHKNB_RS25100"
                     /old_locus_tag="pgaptmp_005020"
     CDS             complement(2036..2551)
                     /locus_tag="ACHKNB_RS25100"
                     /old_locus_tag="pgaptmp_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198362.1"
                     /translation="MFFKTSNPSALAAWKKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILHGPVKWFCVDDVIYLQCEDDSAKRKMTEILSD
                     EFYAAEKRVRG"
     gene            complement(2551..>2861)
                     /locus_tag="ACHKNB_RS25105"
                     /old_locus_tag="pgaptmp_005021"
     CDS             complement(2551..>2861)
                     /locus_tag="ACHKNB_RS25105"
                     /old_locus_tag="pgaptmp_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198363.1"
                     /translation="AADDVQWCSFRKSLPWIEGHDIPSMAWEFAQGVLTCETVYVVAE
                     VDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITARVLRYLIVDRTAAPAGISAEVA
                     "
BASE COUNT          764 a          759 c          600 g          738 t
ORIGIN      
        1 gtgcgttcgg gcattcccat cgcggccagc attttgcggc ggtgccatcg cttgagacgc
       61 tccagcacat ctgaggccag ggcggggtgt tgctccagcc actgccagtt tgccacacct
      121 tcgccgccgt tctgcgcggc cgtctgcgat ttcacccagc ggttaagagc ggtttccgca
      181 ccatcagaga taaagccctg ccggtgcatc actttccaga tggcgcgaat tttggcggtc
      241 accgtacccg gttttaaggc ccggttaacc ggtttctgac gcactttaaa accgcgtttt
      301 ttgaaaacat ccagcacgcg ggataactca ccgggtgaca tatcccggca gctggttttg
      361 ccggtggctg ccagtagtac gaatgtgtag gtctcatcat ccagggcaag ctcgcgcttt
      421 gccacatgaa tagcggatat cagtttccct ctcatgctgt atcaacactc ctgctgatat
      481 gatagatatt actttcccga tcgagctgaa cccgatactc cggaggaatc acaaaaatat
      541 taaaaccatc atcagttttt attcttgagt agtattcccc cttatgatat ctggcaagtg
      601 cattaatcag agaagtcgcc gccagcccaa tcaggtattt tttatgaaaa atactgataa
      661 ctttccggca cagccatcgt gtaatgacga atgcaccgaa aaacgatgtg atatataaac
      721 accagaacaa ctgcgaacta ctgtccatta tctttctcct tattcagtct tctgcgacat
      781 tcggcacagt catcagggct ttcaaatgta tccggctcgc gattatgagc cagatataac
      841 caccgcctac atatactgat attatcgcca gttttaaaaa aatgatgctt cttagccatc
      901 agagggcgcg cccatccagc gggtagacta ttcatttgct ttatcctcca tactggacaa
      961 aatcatctgt attaatgtcg ggcgtggaga tatacgcatt tcattaagcg tctgaagggc
     1021 agcaacagct gcctgtttat cggtgccttt ggcaatttcc gcaagagaat gcacaatcca
     1081 taacggactt aacgccacga ctgaatccag aatcatgtca tccataataa tttctgacac
     1141 atcccgctta tcacattcct tatccataaa agaaacgatt ttttccacat cttccggttt
     1201 catgcctccg gcgataagaa atttgcgata ctcataagac gatcttaatt caatactcat
     1261 attcatctcc tgtaactctg acaaggacgt gatttttctc atttatgttt tccagtaata
     1321 cggatatata tgtcgataag aagattcata ataaacagga agagcgacat cctgataaag
     1381 aaccaaatat ctggcatcat ggacaggttt cctgtaatcg caaatataaa accaaccccc
     1441 aacaccggaa cgaaaattgc gaaaaataca agtctcttta tcatggcgtt aactcccgta
     1501 atacagcgtc ctcggcagtc cggcgatggt gataaataaa attcaccgta ctctcggata
     1561 attcaaattt ctcgccgatt tccctaaagg taagacgccg tggtgattca gcatcccgta
     1621 attcacgaat aagatgaaca tcatcgtcag gtatgcgggt aaaaggcagt aattctccgt
     1681 attttttcat gcttacgcca atatatcgcg cccgattgtg gacagaatcc cggtctcgcc
     1741 ccagatattc tcctatttgc ttaccggtca tcgttctggc attttttcgg ataaatttat
     1801 cttcatcatg tttaaatctt ggacgtttgc atttgagcaa ctccgggtaa gtaccgcgta
     1861 ataaaacaat ccggttatgt acaccccaga aacttctttt taccctgaca gcaatatcct
     1921 taaccggggt ggatggataa agcgcaatca gcagtgcatc ttcttcaggt gtccacgcac
     1981 ggacatgagc gggtgcacgg ccttttcgcc ccatgggttg taaaatcatc atacatcacc
     2041 ccctgactcg cttttcagca gcataaaact catcagacag aatttcggtc attttccgtt
     2101 ttgcggaatc atcttcgcac tgaaggtaaa taacatcgtc aacgcagaac cacttcacgg
     2161 gaccatgcaa tattgctgaa aagtcaatgc ccagccagaa caacagggca tcggttctgg
     2221 catacgtgac gggcgaatat tcacgccaca gactgttaag ttcgtcagag gcaacgcgca
     2281 gggctttggg aatacgtgat gtgcgcggtg tgcagctcca gccattcgac gcagtcggtt
     2341 ttcgccataa gtcgcgatga aaaggatatt tgtcatccat aaagcgcagc cctttaaaac
     2401 aaaagccact gataccggat acaaataccg accggcacgc aacattcagc acggcctcaa
     2461 ggcgttttgc ctcatcctta actttctggc agtcctgctg atattttttc cacgcggcca
     2521 gcgcggaagg gtttgatgtt ttaaagaaca ttacgccacc tccgcagata tcccggcagg
     2581 cgcggccgtc cggtcaacaa tcaggtaacg cagaacacgc gcggtgatat tccagccgct
     2641 taaaccgcag atgatgacac ccaggcgtat gtcgatatac gccatcacaa actggggcac
     2701 gccttctttc atggcctgct catcaacttc agccaccaca taaacggttt cgcaggtcag
     2761 tacgccctga gcaaattccc acgccatcga cggaatatca tgcccctcaa tccacggcag
     2821 ggactttctg aagctgcacc actgaacatc atcagccgca c
//
LOCUS       DAZFVO020000294         2850 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFVO020000294 DAZFVO020000000
VERSION     DAZFVO020000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.296"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(33..233)
                     /locus_tag="ACHKNB_RS25110"
                     /old_locus_tag="pgaptmp_005022"
     CDS             complement(33..233)
                     /locus_tag="ACHKNB_RS25110"
                     /old_locus_tag="pgaptmp_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4198364.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            275..640
                     /locus_tag="ACHKNB_RS25115"
                     /old_locus_tag="pgaptmp_005023"
     CDS             275..640
                     /locus_tag="ACHKNB_RS25115"
                     /old_locus_tag="pgaptmp_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4198365.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            931..1494
                     /locus_tag="ACHKNB_RS25120"
                     /old_locus_tag="pgaptmp_005024"
     CDS             931..1494
                     /locus_tag="ACHKNB_RS25120"
                     /old_locus_tag="pgaptmp_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4198366.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPLPVVIS
                     PGIEYMDDGLPDPVKAMGRLLVENINTDPRLALDVAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGSG"
     gene            1491..>2850
                     /locus_tag="ACHKNB_RS25125"
                     /old_locus_tag="pgaptmp_005025"
     CDS             1491..>2850
                     /locus_tag="ACHKNB_RS25125"
                     /old_locus_tag="pgaptmp_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4198367.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEDTFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSL"
BASE COUNT          635 a          688 c          891 g          636 t
ORIGIN      
        1 gacagtgggt acctgctcct gtaaccgaac gttcatttct cgttatttgt catgctggcc
       61 gggcgcaggt gcgttgcatc tgttgccagc cttctccggc aggcttcaat aacccacgct
      121 gaaaagttac cggacccttt atgctcaagg gcgatgttga tctgttcaat catgtgattg
      181 gggaaacgga tattgcgggt tgtggttctg cgggtccggt ttttcgatga catatttatt
      241 tcctttactg attgccttat gacggggatt ttacatggct gagcttcgta cactccagag
      301 cagaatcaaa acactgaata cccgacgggt gaatattctg aagggtgaac agcgtcgtgt
      361 cagtggcagt gcacgtgttt ccctcaagcg tcatatctgg ctcagggatg ccgggcagtg
      421 ctgtctctgt ggtcgtgtgg ttgacctctg tgacagtgaa ctcgatcacc gaattgcact
      481 tcagttcggt ggtggtaatg aggagacgaa tctctggacg ctctgtaccg aatgccatcg
      541 acaaaagtct gctcgtgaag cggcgggtgg tatgccggac ccgacactgc cggaggtgtc
      601 cggaggtagt ggcagagcgg acgacatcat cggactgtaa cccgacccgg ggggggtatc
      661 atccggcgta aaaaacgatc gctccggaca ccgcgccccc tctcacgcag agaaaaaatt
      721 cccgtttcag gtcagttaac atgttaactg gctgcacggg catttttgca gtttttatct
      781 ttattattca gtttgttgcg cggaaaaaat gttaacaggc tttttcagca aatgttaacc
      841 aggcagcagt taacatttgc ggcatgagac gccgggaaaa atgggctgaa ccatacccgg
      901 ctgagtgcat tctggaccct ggaggaggct gtgctgacaa cgcaaaaacg aaaatttgcg
      961 ctggcgctca tgtccgggaa aaacaaaaca gcgtcagcca ttgccgccgg ttattcggcg
     1021 aagacagcca gggttaaagg ctcgcagctg gcaaaagatc cggaggtgct cgcgtttata
     1081 gcccgtaagc agtgcgagac ggtagaggtg gatgaggttc ctgtttaccg gcagaaaaaa
     1141 tcagagcagg aggataaacc ccgtcgccgt gaggcggctg caataccaca gccggacgaa
     1201 acaaatccgg agatgccact gcccgtggtg atatctcctg gtattgagta tatggatgac
     1261 ggtcttcccg atccggtgaa agcgatgggg cgtcttctgg tggagaacat taataccgac
     1321 cccaggctgg cgctggatgt ggcttataag ctggcacagt tcacgcacca caaaaaaggg
     1381 gatgccggta aaaaatcggc aaaaggtgac gcggcgaaaa aagcggctaa ccgttttgcg
     1441 gtgccaccac ccccccgact ggtggtgaat aatgataatg agggcagcgg atgatacctg
     1501 tgtggagcac ggcctgcccg gactgggcag agcgcctgaa aaaggggctg tcgattattc
     1561 cggctccgat ttacccggac caggccgcac atgccctggc gatttttaaa caactgcgga
     1621 ttgtggatgc accgggcagc ccgacgttcg gggagtcctg tgcaccgtgg gtgtttgatc
     1681 tggtggctgc cctgtttggc tcctacgatg cgcagaccgg tgtacgccat ataaaggaag
     1741 tgttcattct gatacccaaa aaaaacagca agtccacgct ggctgccggg atcatgatga
     1801 cggcgctgtt actgaactgg cggcaggcgg cgggctacac cattctggcc ccgaccgtgg
     1861 aggtggcggc taatgccttc aatcctgcca gggatatggt acgacgggac gatgatctgg
     1921 atgacctctg tcaggtgcag acacatatcc ggaccatcac ccatcgggtg acggacacca
     1981 ccctgaaggt ggtggcagcc gatccgaata cggtgtccgg tatcaagtcc gtggggacac
     2041 tgattgatga actgtggctg tttggcaagc agtacaaagc ggaggacatg ttacgtgaag
     2101 ccataggtgg ccttgcctcc cgcccggaag ggtttgtggt gtatacgacc acccagtcga
     2161 atgaaccgcc cgccggggtg ttcagacaga aactgcagta cgcccgggat gtgcgcgacg
     2221 gcaaaattca tgatccgcac tttctgccgg tgatatttga gcatccgccg gagatggtgg
     2281 agcgcggtga gcacctgctg atggaaaacc tcgccatggt caatccgaat ctcggttatt
     2341 cggtggatga ggcctttctg taccgggagt atcgtaaagc ccgggaagcc ggtgaagata
     2401 cattccgggg gttcatgtca aaacacgcca atgtggaaat tggtcttgcc ctgcgttctg
     2461 accgctgggc gggtgcggat ttctgggagc agcagggcag gcgcgtcagc ctggacgata
     2521 ttctgcagcg cgctgatgtg gtgacggtgg ggattgacgg cgggggcctg gatgatctgc
     2581 tgggaatgta cgtgattggc cgtgacaggg aaacccgcga atggctgggc tggggccatg
     2641 cctgggcgca tgaaaccgcg gtggtcagac ggaagagtga ggcatcccgt tttcaggatt
     2701 ttgtggcctg tggagacatg acgattgtcc gtcgggtcgg ggatgacacg gcggaagtgg
     2761 cggagtatgt gcgtcgtatt catgaggctg agttactgga tcatatcggt attgacccgt
     2821 caggtgtggg gcagattctg gattcactgg
//
LOCUS       DAZFVO020000295         2845 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFVO020000295 DAZFVO020000000
VERSION     DAZFVO020000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.297"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            24..500
                     /gene="agaB"
                     /locus_tag="ACHKNB_RS25130"
                     /old_locus_tag="pgaptmp_005026"
     CDS             24..500
                     /gene="agaB"
                     /locus_tag="ACHKNB_RS25130"
                     /old_locus_tag="pgaptmp_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HGU4198368.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            539..1342
                     /gene="agaC"
                     /locus_tag="ACHKNB_RS25135"
                     /old_locus_tag="pgaptmp_005027"
     CDS             539..1342
                     /gene="agaC"
                     /locus_tag="ACHKNB_RS25135"
                     /old_locus_tag="pgaptmp_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HGU4198369.1"
                     /translation="MHEITLLQGLSLAALVFILGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1332..2123
                     /gene="agaD"
                     /locus_tag="ACHKNB_RS25140"
                     /old_locus_tag="pgaptmp_005028"
     CDS             1332..2123
                     /gene="agaD"
                     /locus_tag="ACHKNB_RS25140"
                     /old_locus_tag="pgaptmp_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HGU4198370.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2124..>2845
                     /gene="agaI"
                     /locus_tag="ACHKNB_RS25145"
                     /old_locus_tag="pgaptmp_005029"
     CDS             2124..>2845
                     /gene="agaI"
                     /locus_tag="ACHKNB_RS25145"
                     /old_locus_tag="pgaptmp_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HGU4198371.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWL"
BASE COUNT          691 a          673 c          722 g          759 t
ORIGIN      
        1 aactttatgg tgaggattac acaatgagca gtccaaatat tctcttaacc cgtattgata
       61 accgtctggt tcacggtcag gttggcgtga cctggacatc caccatcggt gcgaatctgc
      121 tggtagtcgt ggatgatgtt gtcgctaacg atgatattca acagaaatta atgggtatta
      181 ccgcagaaac ctacggcttt ggcattcgtt tctttactat cgaaaaaacc attaacgtca
      241 tcggcaaagc tgcaccgcat cagaagatct tcctgatttg ccgtacgcca caaacggtac
      301 gtaaattggt agaaggtggc attgacctga aagatatcaa cgtcggcaat atgcatttct
      361 cggaagggaa aaagcaaatc agcagtaaag tttatgtcga tgaccaggat ctcgcggact
      421 tacgttttat taaacaacgt ggcgtgaatg ttttcattca ggacgtccct ggcgatcaaa
      481 aagaacaaat ccctgactaa atctaaaatc gccttaatat tggtttgagg taataaaaat
      541 gcatgaaata accctacttc agggattatc cctagcggcg ttagttttca ttctggggat
      601 tgatttttgg ctggaagcct tatttttatt ccgcccgata atcgtttgta ccctaactgg
      661 cgctattctc ggtgatattc agactggctt aattaccggt ggtctgacag agttggcttt
      721 cgccggatta acccctgcag gtggtgttca gccgcccaac ccgattatgg cgggtctgat
      781 gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa acagcaattg gtcttggcct
      841 gccgtttagt ttgttaatgc agtacgtcat tctgttcttc tattccgctt tctcattatt
      901 tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg gcagcgtttt cccggcttaa
      961 ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg attgctttcc tctgtactta
     1021 cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg cccgcctggt tgacccacgg
     1081 ctttgaagtg gctggcggta ttctgcctgc cgttggtttt ggcttgctgc tgcgcgtaat
     1141 gttcaaagcg caatatatcc cttacctgat cgccggtttc ctgtttgttt gctacatcca
     1201 ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc tttgcggtgt atgagttttt
     1261 caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa atgaagaaga
     1321 ggactacagc aatgggatct gaaatcagta aaaaagatat cacccgtctg ggctttcgtt
     1381 cgtcgctgct gcaagcgagc tttaactacg aaaggatgca ggcgggcggt tttacctggg
     1441 cgatgttgcc gatcctgaaa aagatttata aggacgacaa accgggctta agcgcggcga
     1501 tgaaagataa cctcgaattt attaataccc acccgaatct ggtcggattc ctgatggggt
     1561 tattaatttc gatggaagaa aaaggagaaa accgcgacac cattaaaggc ctcaaagtgg
     1621 cactgtttgg cccaatcgcc gggattggcg atgcgatttt ctggtttact ttgttgccga
     1681 ttatggcggg aatttgctca tcatttgcca gccagggaaa cctgctgggg ccgattctat
     1741 ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg ctggacccac gtcggttatt
     1801 cagtcggcgt gaaggcgatc gataaagtgc gagagaactc gcagatgatt gctcgctcgg
     1861 caaccatcct cggaatcacg gtaatcggcg ggctgatcgc ttcgtatgtg catattaacg
     1921 tggtgacatc gtttgccatc gacagtaccc acagcgtcgc gctgcaacag gatttcttcg
     1981 ataaagtctt cccgaacatt ttaccgatgg cctacaccct gctgatgtat tacttcctgc
     2041 gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac ttttgtgctc tctattgttt
     2101 gttccgcatt cggcattttg taaatggaac gaggcactgc ttctggcggt gcctctttac
     2161 ttaaggaatt tcatcctgtg caaacccttc agcaagttga aaactatacg gcgttaagtg
     2221 aacgagccag cgaatattta ttggctgtga tccgtagcaa accggatgcc gtgatttgcc
     2281 tggcgaccgg agccacgcca ttactgacgt atcattatct ggtagaaaaa atccaccagc
     2341 agcaggttga tatcagccag ctcaccttcg tgaagctcga cgaatgggtg gatctgccat
     2401 taacgatgcc tggcacctgc gaaactttcc tacaacagca tatcgtgcag ccgctggggc
     2461 tgcgtgaaga ccagctcatc agctttcgct ccgaagagat aaatgagaca gagtgcgaac
     2521 gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt atgcgttcta ggattgggga
     2581 aaaacggtca tcttgggctg aacgaaccgg gagaaagcct acaaccggcc tgccatatca
     2641 gtcaacttga tgccagaaca cagcaacatg agatgttaaa aaccacgggt cgccccgtga
     2701 ctcgtgggat caccttaggc ctgaaggata ttctcaatgc ccgcgaggtt ttgttattgg
     2761 tgactggcga aggaaagcag gatgcgacag aacgttttct cacggctaaa gtctccaccg
     2821 ctatcccggc ttcgttttta tggct
//
LOCUS       DAZFVO020000296         2817 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFVO020000296 DAZFVO020000000
VERSION     DAZFVO020000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.298"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..354)
                     /locus_tag="ACHKNB_RS25150"
                     /old_locus_tag="pgaptmp_005030"
     CDS             complement(<1..354)
                     /locus_tag="ACHKNB_RS25150"
                     /old_locus_tag="pgaptmp_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4198372.1"
                     /translation="MTRTERLLELLQILRAQRYPITASILSERLGISVRSLYRDIKTL
                     QHQGVCIEGGAGIGYIVKSDFHLPPLNLSHEEINAITLGLNWVSHNTDYNFKITAKNA
                     LAKIHAVIPSELKNLI"
     gene            573..1175
                     /locus_tag="ACHKNB_RS25155"
                     /old_locus_tag="pgaptmp_005031"
     CDS             573..1175
                     /locus_tag="ACHKNB_RS25155"
                     /old_locus_tag="pgaptmp_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGU4198373.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVAALIKL
                     GNLHSKDFLFRSRVGTNQHISTRQYNRIFHGWIEKLGLEGSLYSTHSMRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
     gene            1327..2448
                     /locus_tag="ACHKNB_RS25160"
                     /old_locus_tag="pgaptmp_005032"
     CDS             1327..2448
                     /locus_tag="ACHKNB_RS25160"
                     /old_locus_tag="pgaptmp_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198374.1"
                     /translation="MAITVYSRTFKKELDMVQLLNHYSSDNALNFSDFKEFVAFDAEC
                     PICNVSGAIVVSEGYSKRTNKIVSQSHFAFRTKDGTDAHKVFCDHYSGDDKQIDSSRD
                     GFIKFGTSGSEITSIIRELVCRGIEHDEFNQEDIRNMRQWFTELRQSGTFSVNYSPHM
                     INLIRASMYSRKGESKYIVDEERKNKSWFDINDEVYRSLRFKYPSLMIDMTNENNSVF
                     YNLVNSTTFTKQAHRLVARDRGYQVYDRRLLKEKYEATIRVAQKITRHHEFLFRKIRG
                     ISAVKKNNPLLAVSALLLFVSDWNETTAINKFIKLCGVGKSKNNDEGNVIGLNPFIHY
                     DVWNVIHQLKDLMVTLPDFSNLDEEFDREKSRLIELYKL"
BASE COUNT          899 a          518 c          584 g          816 t
ORIGIN      
        1 aataagattt ttcaattcac tgggaatgac ggcgtgtatt ttcgcaagcg cattctttgc
       61 ggtaattttg aaattataat ctgtattgtg cgatacccaa tttaacccaa gtgtaatagc
      121 attaatttct tcatgagaaa gatttaaggg gggcaaatga aagtcagatt taacaatata
      181 accaattcca gcaccacctt caatgcatac tccttgatgc tgcagcgttt ttatatctct
      241 gtaaagggag cgtacactta ttcccaatct ttctgagagg atggatgctg tgatcggata
      301 tctttgtgcc cttaatattt gtagtaactc gagaagtctt tctgttctag tcatgttgat
      361 aaatccattt tgaggcgatt tcttaaagca tctgttagat aaacatatta gtacaaagca
      421 agtgtacatg gctaagctga actgctaccg atgattaata cagcgctatg ttcgtaatgc
      481 gtacataagc ggtgatcatg agtgtaaaca tccgcttttc gcttataagg gacaaccata
      541 ctcaaatttc ccacattgca ggagatttga gtatgaatac gtcaccgtgg aacaaagacc
      601 gtatcatcgg ccaaaaaaga ccacttcaga tatctcatat ctgggggatc cgaatccggc
      661 ttgaactgga aggtaaaacg cgcgatttag ctctgttcaa tatggccctg gacagtaagc
      721 ttcgaggctg tgatctggtc aaactcaaag tatcggatgt ggcatatggt agttcggttt
      781 caagcagagc aacggtgttg caacagaaaa ccggtagccc tgtgcaattc gagataacca
      841 aagggacaag agaagctgtt gctgcattga taaagcttgg caatttgcac agtaaagact
      901 tcttgtttcg gtctcgggtc ggaactaacc agcacatttc aacccggcaa tacaaccgaa
      961 tctttcatgg gtggatagaa aaacttggtc tcgaaggttc actttacagc acacattcaa
     1021 tgagaagaac aaaaccttac ctgatctaca agaaaaccaa gaatctccgg gtgatccaac
     1081 ttctgttggg tcataagaaa ctggaaagca cagtccgtta tctgggcatt gaagtcgatg
     1141 atgcgttaga gatctctgaa tcgattgaag tctaaggttg tcagggctgc aacagcagcc
     1201 ctgtgccagg agcggacgtc tagttcgctg aaccacaaaa agtcgtttct agttatatta
     1261 ctggctcatt agtatcttta tgcgataata tttcatacaa ccaaaattat gatgaaggga
     1321 gttcaaatgg caattacggt ctattctcgt acattcaaaa aggaattgga catggttcaa
     1381 ttgttaaacc attactcatc agataacgca ctcaatttca gtgatttcaa ggaatttgtt
     1441 gcctttgatg cagagtgccc aatttgcaat gtaagcggtg cgattgttgt ttcagaaggt
     1501 tattcgaaaa gaacaaataa gatagtcagc caatctcatt ttgcgtttag aacaaaagac
     1561 ggaactgatg cacacaaagt attttgcgat cattacagtg gtgatgataa gcaaatagat
     1621 tcatcaaggg atggttttat aaaattcggc acctcaggtt ctgagattac aagtataatt
     1681 cgcgagcttg tatgtagagg tattgaacat gatgagttca atcaggaaga tattcgaaac
     1741 atgcggcaat ggtttacaga attacgccag tcaggcactt tttctgttaa ctatagccct
     1801 cacatgatca atctaataag ggccagcatg tattcccgta agggagaaag taaatatatt
     1861 gtagatgaag agaggaaaaa taaatcttgg tttgatataa acgatgaagt gtatcgttcg
     1921 ttaagattca aatatccatc attgatgatc gacatgacta atgaaaataa ttctgttttc
     1981 tataatcttg tgaatagtac cacatttact aaacaagcac atcgattagt agcacgagat
     2041 cgaggttatc aggtttatga tagaaggttg cttaaagaaa aatatgaagc aacgataaga
     2101 gttgcacaaa aaataaccag gcatcacgaa tttcttttta ggaaaatcag gggtatatcc
     2161 gctgttaaaa aaaacaaccc ccttttggct gtatctgcct tgcttctttt tgtctcggac
     2221 tggaatgaaa caactgcaat caacaaattc atcaaattgt gtggtgtagg gaaatctaaa
     2281 aataatgatg aggggaatgt aattggctta aatcccttta ttcattacga tgtctggaat
     2341 gttattcatc aacttaagga tttaatggtt acacttccag acttctcaaa tcttgatgaa
     2401 gaatttgaca gagaaaaatc cagattaatt gaactttaca agctctaaca taaactagca
     2461 ggctgcgtat acagtctgct aattttcata gtaaagactt catgcttcag tctcgagttg
     2521 gagctatccg tcacacatca cccaggcaat tcaaccgaaa tttttcatgg ggggtagaga
     2581 agttttgtct cgaagatttg ctttacagca tactaatcat tgaacctctg ttgaaccata
     2641 agaaactggc aagcacaatc cgttgcttgg gcattgaatt cgacgatgcg tcgtatagct
     2701 ctgacttgaa caaaacataa ggtttttagg gctgcaacag cacctctgtg tcataggcgg
     2761 aagttaacaa acattaaaat ctctaaatct gtagggatca gactccttct ataaaag
//
LOCUS       DAZFVO020000297         2814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFVO020000297 DAZFVO020000000
VERSION     DAZFVO020000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.299"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            215..940
                     /locus_tag="ACHKNB_RS25165"
                     /old_locus_tag="pgaptmp_005033"
     CDS             215..940
                     /locus_tag="ACHKNB_RS25165"
                     /old_locus_tag="pgaptmp_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198375.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            898..1575
                     /gene="bglJ"
                     /locus_tag="ACHKNB_RS25170"
                     /old_locus_tag="pgaptmp_005034"
     CDS             898..1575
                     /gene="bglJ"
                     /locus_tag="ACHKNB_RS25170"
                     /old_locus_tag="pgaptmp_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HGU4198376.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1613..2401)
                     /gene="fhuF"
                     /locus_tag="ACHKNB_RS25175"
                     /old_locus_tag="pgaptmp_005035"
     CDS             complement(1613..2401)
                     /gene="fhuF"
                     /locus_tag="ACHKNB_RS25175"
                     /old_locus_tag="pgaptmp_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HGU4198377.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2542..2778
                     /locus_tag="ACHKNB_RS25180"
                     /old_locus_tag="pgaptmp_005036"
     CDS             2542..2778
                     /locus_tag="ACHKNB_RS25180"
                     /old_locus_tag="pgaptmp_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HGU4198378.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          745 a          597 c          708 g          764 t
ORIGIN      
        1 ttttaaatgt ctccaggcta tttcaccagg ttatcatttg atgctaacct tctgacacat
       61 gcagtggagt tgttgtgcag caggagtatg ctgatatgaa agtgaatgct aaggataatt
      121 tattcgctta atctattaat ttgctggaaa catttaagga atgaatatcc tgaataaacc
      181 atcatgattt ttagacagtg gatgtggagg aaatatgttg ccaggatgct gcaaaaatgg
      241 aattgttatc agtaaaatac ctgttatgca agcagggtta aaagaggtca tgaggactca
      301 cttccctgaa tatgaaataa tatccagcgc ctctgcggag gaccttacct tattacaatt
      361 acgtcgttcc ggattagtca ttgctgattt agccggtgaa agtgaagatc ctcgttctgt
      421 ttgtgaacat tattattctt taatctcaca atatcgggaa attcactggg ttttcatggt
      481 gtcacgctcc tggtattccc aggcagtaga attgctcatg tgccctacgg cgacgttatt
      541 gtctgatgtt gaacccattg agaatctggt caagaccgta cgttccggca atacgcacgc
      601 agagcgtatc agcgccatgc tgacctctcc ggcaatgaat gaaactcatg attttagcta
      661 tcgctccgtc attctcactc tttcagagcg caaggtactg cggctattag gtaaaggatg
      721 gggcatcaac cagatagctt cgttgcttaa gaaaagtaat aaaactatca gcgcccaaaa
      781 aaacagtgcg atgcgtcgac tggcaattca cagcaacgct gaaatgtatg catggataaa
      841 tagcgcgcag ggtgcaagag aacttaactt gccttctgtt tatggagatg ccgcagaatg
      901 gaacacagcc gaattaagaa gagaaatgtc gcactcatag aaaaatgcgt catgagcagt
      961 atcggtattg agagtttatt cagaaagttt gcgggtaacc cttataagct ccatacctat
     1021 accagtcagg agtcatttca ggatgccatg tcgcggatct catttgcggc ggtcattttt
     1081 tctttttctg ccatgagaag tgagcgcaga gagggattat cttgcctgac tgaactggcg
     1141 attaagtttc cgcgtacccg gcgtttagtt attgcggatg atgatattga agctcggctg
     1201 attggttcat tgtcgccatc accgctggac ggtgtattaa gtaaagcgtc aacgctggag
     1261 atttttcatc aggaactctt tttgtcatta aatggtgtac gtcaggcgac cgaccgactg
     1321 aacaatcagt ggtacattaa ccaaagccgg acgctaagcc cgacggagag agaaatattg
     1381 cgctttatgt cgcgtggcta ctcaatgaca caaattgccg agcagcttaa acgcaatatc
     1441 aaaacgatcc gtgcacataa atttaatgtg atgtcgaaac tgggcgtcag ttctgatgca
     1501 gggttgttgg aggccgcaga tattctgtta tgtatgcggc attgcgaagc aagtaatgtg
     1561 ttgcatccct attaatccgc atgatgccgg gtttacttcc ccggcagtgc tttcatttca
     1621 gcgtacaatc gccacattgc tgcacatccg gtaagcgata acgctggcag caggtccggc
     1681 gcaccagcag gccgtcgcgt agcaccacgg tacgccagag tggattatct tcaccggtag
     1741 taagcgtttt ctcaaaaaag agggcatggc gcagcgattc aaccgtcgcc tcgccgagca
     1801 gttgcttcat ctcagtgaga taccagttga tcaaataacc ggtattactc cagataagtt
     1861 tgccgttgat ctctccggtc gcttctagtg cctgcacaac cggaaccagc gcctggctga
     1921 ttaacgtttc cattcgctgc tgcggcgaat gtggtgttgc gtttttatct tcacacacat
     1981 cgacccagaa acaggcgacg cgtccggttt cgtgaaactc agcatggaaa tgttccggcg
     2041 acacatctaa tgccttttcc tgcgtcagta gcgccagcat taatggtggc accatcaggc
     2101 cgatatacca ttgtgcccat agtgagatca gcggtttgtt ctcgcggatc atcgttggtt
     2161 ggttgcgata gatatgatcg gaatagaccg cgagtagaga acttagcgca ttcggtgatg
     2221 accattgcgc cagcgtcatg gcgttaagcg gagcgggttc atccaggcgg ataaactcga
     2281 gcaaatgttc acgatgtttg gcgatcgtcg cacgtacggc ttgtgcaagc ccagcatcct
     2341 gcggctggag atgcgttcgc cagatgatat cttcatagag tggtgcggaa cgataggcca
     2401 taatcgggat agtaatctaa atgataatga ttgctaatcc tagcgatagg tttacccgat
     2461 agcaagggat ttctctggct tgcaaatgat aaaaattatc atatgatatt ggttatcatt
     2521 atcaatgaaa gagatgaaac catgttgcaa cgtatgctgg gcagtggctg gggagtgttg
     2581 ctgccaggat tgctgattgc agggctgatg tatgcggatt tatcgccgga tcagtggcgg
     2641 attgtcattc tgatgggatt agtattgacg ccggtgatgc tgtatcacaa acagttacgg
     2701 cattacgttt tgctaccatc gtgcctggcg cttattgctg gcatcatgct gatgataatg
     2761 aatttgaatc agggatgaaa aatcaaggaa gaaacaagaa aggaagtaaa gata
//
LOCUS       DAZFVO020000298         2775 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFVO020000298 DAZFVO020000000
VERSION     DAZFVO020000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.300"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..396
                     /locus_tag="ACHKNB_RS25185"
                     /old_locus_tag="pgaptmp_005037"
     CDS             <1..396
                     /locus_tag="ACHKNB_RS25185"
                     /old_locus_tag="pgaptmp_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HGU4198379.1"
                     /translation="QRVLQSASKLRQSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVR
                     ETRPVRNFYVGTNDPRVILCLTRQAEELESRGLYRRAATVWMAAFRESHSQPERNNFL
                     ARRERCLRKSSKRAVSGDEWYLSGNYVGA"
     gene            396..1049
                     /locus_tag="ACHKNB_RS25190"
                     /old_locus_tag="pgaptmp_005038"
     CDS             396..1049
                     /locus_tag="ACHKNB_RS25190"
                     /old_locus_tag="pgaptmp_005038"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HGU4198380.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCTAYYTAEDNALVHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            1046..1372
                     /locus_tag="ACHKNB_RS25195"
                     /old_locus_tag="pgaptmp_005039"
     CDS             1046..1372
                     /locus_tag="ACHKNB_RS25195"
                     /old_locus_tag="pgaptmp_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HGU4198381.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            1369..1764
                     /locus_tag="ACHKNB_RS25200"
                     /old_locus_tag="pgaptmp_005040"
     CDS             1369..1764
                     /locus_tag="ACHKNB_RS25200"
                     /old_locus_tag="pgaptmp_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGU4198382.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            1927..2742
                     /locus_tag="ACHKNB_RS25205"
                     /old_locus_tag="pgaptmp_005041"
     CDS             1927..2742
                     /locus_tag="ACHKNB_RS25205"
                     /old_locus_tag="pgaptmp_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HGU4198383.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     PTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT          703 a          668 c          776 g          628 t
ORIGIN      
        1 cagcgcgttc tgcagtcagc aagcaagtta cgtcagagcg ggcgtgtggc tcaccgttgt
       61 gaaggagata cacgcagaca tttcccgcgc ctgactgaga gagcgcagga accggaacca
      121 caaccagttc gtgaaaccag acctgtgcgc aatttctatg tcggcactaa cgatccccgg
      181 gtgattttgt gcctgacccg ccaggctgaa gaactggagt ccaggggctt ataccgtcgt
      241 gctgcaaccg tgtggatggc ggcattccgt gaaagccact cccagccaga acgaaacaat
      301 tttctggcac gtcgtgagcg gtgcttacgg aaaagcagca agcgcgctgt atcgggtgat
      361 gagtggtatc tgtcagggaa ttacgtgggg gcttaatgag taataaatat tgccaggagc
      421 tggtggagct gcggaacaaa ccagcccatg aactgaagga agtgggcgat cagtggcgca
      481 cgccggataa cattttctgg ggaattaaca ccctgtttgg cccatttgtc ctggatctgt
      541 ttactgacgg tgataacgcc aaatgtaccg cgtattacac ggcggaagac aacgcgctgg
      601 tgcatgactg gtcagaacgt cttgcggagc ttaaaggtgc tgcctttggt aatcccccat
      661 acagccgcgc cagtcagcat gaggggcaat acatcaccgg catgcgttac atcatgaaac
      721 atgccagtgc catgcgtgat aaaggcgggc gctatgtttt cctgatcaaa gctgccacca
      781 gcgaagtgtg gtggccggaa gatgcagatc atattgcttt tattcgcggg cgtattggtt
      841 ttgaactgcc tgcctggttt atcccgaaag atgagaagca ggtgccgaca ggcgctttct
      901 tcgctggtgc tattgctgtt ttcgacaaga cctggaaggg accggcaatc agctacatcg
      961 ggcgtgatga acttgaggca tgtggtgagg cgtttctggc gcaggttcgc cagcaggcgg
     1021 aaaaactggt cagggagatg gcggcatgac gacgttaact caatgccagc agcaggtgct
     1081 ggatatgctg atttcttatc agaaagaacg tggcttcccg ccaaccaatc aggaggtggc
     1141 aaccatgctg ggataccgtt cagtgaatgc agcggtggag catcttcgcg cactggagaa
     1201 aaaaggcgtc atcacgataa agcgtggtgt ggcccggggg atcacgcttc ataccgtggt
     1261 gaaggacgac gacagcgagg cggtcgggat tatccgctca ctgcttgccg gtgaggaaaa
     1321 cgccaggctg cgtgcagccc attggttaca tgagagaggc ctgaaagtat gaagctgatc
     1381 ctgccttttc cgcccagcgt gaacacgtac tggcgacacc ccaacaaagg ggcgtttgct
     1441 ggtaagagcc tgataagcgc ggcggggcga aaattccaga gcgcggcgtg tgcagcaata
     1501 gttgagcagt tacgtcgtct gccgaaacca acgtcggcac ctgcttcagt ggagatcgtg
     1561 ttgtttcctc cggataaccg gatccgcgat ctggacaact ataacaaggc gctgtttgac
     1621 gccctgaccc acgcgggggt gtgggaagac gacagtcagg tgaaaagaat gctggtggag
     1681 tggggaccgg ttatcccgga ggggaaggtc gagatcacta tcagtaagta cgaaaaagcg
     1741 agttgcaaat tagcaactcg gtaacggaat tgagcaacac cctaaatttg ggtattacct
     1801 cgttaaagat actgtattta tgaacagtgt atccttgata actattaaaa atcgcagtaa
     1861 gttcatcctg catcaacgaa aagggagtgc agtcccgctc gtggataaaa atttgtggag
     1921 aaaccaatga atcagttgct tgtaattgat ggcgtttctg tgcgccagta cttcgaatct
     1981 aactactgtc ttaacgacct tcagaaagct gctcttcttg ccgctggtga gaatcgctcc
     2041 tcccgttcgc tggaagttca cgagtttatg cgtcgtcctg aaacgaaggc tcttgtggaa
     2101 ttattggaag aagaaactac gggagattcc cgtagtattc ctgtcatcac cattcagggg
     2161 cgcaatggtg ggacgtatgt ctgtaaagag ctggtctatg catatgcaat gtggatcagc
     2221 ccggcattca gcttaaaagt gatacgtact tttgatgcgc ttcataattc atcaccagaa
     2281 gaaaccacat ccgacaaaat taaatccggg gtcattctgc ttgaatcagc agcaaagact
     2341 ctaaatctgt caaactcctc gaaacttggt gcataccaga aattatcaaa ggtagctggt
     2401 cttcctgaac ttatgccgat ctatgccatt gatgcacctg ctgatgcgcc agatggttca
     2461 agccgcccta cgctgtcgct gagtgcactg ctgaagcagt atggtatccg cctgacggct
     2521 aatcaggcat atcaccagat ggcgaagctg gggatcgttg aacaacgcga acgatacagc
     2581 cctaccgcga ttaacaacat caaaaaattc tggtcgctga ccgcgaaagg ctgcatgttc
     2641 ggcaagaaca tcaccagtcc tgcaaatccg cgcgagacgc agccgcattt cttcgaatcc
     2701 cgattccctg agctgttaaa gctgctcgat accgttcatt gaggtgaccg tgagagcact
     2761 actgacccct gaaat
//
LOCUS       DAZFVO020000299         2772 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFVO020000299 DAZFVO020000000
VERSION     DAZFVO020000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.301"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            203..787
                     /locus_tag="ACHKNB_RS25210"
                     /old_locus_tag="pgaptmp_005042"
     CDS             203..787
                     /locus_tag="ACHKNB_RS25210"
                     /old_locus_tag="pgaptmp_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198384.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            892..1632
                     /locus_tag="ACHKNB_RS25215"
                     /old_locus_tag="pgaptmp_005043"
     CDS             892..1632
                     /locus_tag="ACHKNB_RS25215"
                     /old_locus_tag="pgaptmp_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HGU4198385.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1667..2772
                     /gene="htrE"
                     /locus_tag="ACHKNB_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /pseudo
     CDS             1667..2772
                     /gene="htrE"
                     /locus_tag="ACHKNB_RS25220"
                     /old_locus_tag="pgaptmp_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          795 a          577 c          594 g          806 t
ORIGIN      
        1 gcttaatttt gaattaagaa aatttaattt gttcaaaagc ccttaaagtg cacaataggc
       61 gtcctaatga acaatggttg taaattttgg taacaaatat cattgctcat taatatctag
      121 catctgctta taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat
      181 ggaattttat tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat
      241 gctggcaatg gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag
      301 tggtctggtt gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct
      361 gatcctgctg caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atgacaccgt
      421 tggcgctaaa gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc
      481 aggtagtaca gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct
      541 gaacaacgac atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat
      601 tgatggttcc actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc
      661 cctggatgca acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt
      721 cgcagaccaa acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta
      781 tcagtaagta ttcaccaatt aaattgattg aatgaatata cagggaatag tgttaataat
      841 ttctattttt atattattcc ctgttttaat taactctgtc agggatggtt tatgtttttt
      901 aacaccaaac atacaacagc tttatgcttc gtaacctgta tggcttttag ttcatcgtct
      961 attgcggaca ttgtcatttc gggtactcgc gtaatatata aaagcgatca aaaaagtgtc
     1021 aacgtacgtc tggaaaataa agggaataac ccgttgcttg tccagagttg gttagatact
     1081 ggcgatgaca acgctgagcc tggcagtatt acagtccctt ttactgccac gccgccagta
     1141 tcgcgtattg atgccaaacg tgggcaaaca atcaaattaa tgtacacagc cagcacctca
     1201 ctgcctaaag acagagagag cgtgttctgg tttaacgtac tggaagttcc accaaaacca
     1261 gatgcagaaa aggtcgcgaa tcaaagtctg ctgcaactgg catttcgcac acgtataaaa
     1321 cttttctatc gcccggatgg attgaaggga aatccctctg aagccccgtt agccctgaag
     1381 tggttctggt caggttcaga aggtaaggcg tcattgcgag tgaccaatcc aaccccttac
     1441 tacgtctctt ttagcagtgg tgatttagaa gctagcggta aacgctatcc gattgatgtg
     1501 aaaatgattg caccatttag tgatgaggtc atgaaagtca ctggccttaa tggcaaagca
     1561 agttctgcaa aagtacattt ttacgccatt aatgattttg gtggcgccat tgaaggtaat
     1621 gccagtctgt aatcaggcaa ggatataatt ccgcaggaag cataacgtga ctataaaacc
     1681 aactaaaaat tatcataatc atctgacccg tatcgctacg ttttgcgcgc tgctgtattg
     1741 caatagtgct ttctgtgctg aacttgttga atatgaccat accttcctga tggggcagaa
     1801 tgcctcaaat attgatctca gccggtatag tgaaggtaac cctgctatac cgggtatgta
     1861 cgacgtcagt gtttatgtca acgatcaacc tattattaac caaagcatta catttattga
     1921 aattgaagga aaaaaaacgc acaagcctgt atcacactta agaatttatt gcagtttcat
     1981 ataaattctc ccgatataaa taacgaaaaa gccgttctgc ttgccaggga tgaaacactc
     2041 ggcaattgcc ttaatttgac ggaaattatc cctcaggctt ctgttcgtta tgacgttaac
     2101 gagcaacgtc tggatataga cgttcctcag gcatgggtaa tgaaaaatta ccaaaactat
     2161 gttgatccat cgttatggga aaatggcatt aatgcggcca tgttgtcata caacctcaac
     2221 ggatatcata gtgaaactcc tggtcgaaga aatgacagta tttatgctgc ttttaacggt
     2281 gggatgaatt taggtgcatg gcgactgcgt gcctcaggca actacaactg gatgaccgat
     2341 tctggcagta attatgattt taagaatcgg tatatacagc gtgatatcgc ctcgctgcgt
     2401 tctcaactca ttcttggtga gtcttataca acgggcgaaa cctttgattc tgtcagtatc
     2461 cgcggcattc gtttatacag cgacagccgc atgctgcctc cgacattagc gagttttgcg
     2521 cctatcattc atggcgttgc taataccaac gccaaagtca ctattacgca aggtggctat
     2581 aagatttatg aaacgactgt gccgccaggc gctttcgtca ttgatgatct gagtccgtca
     2641 ggttacggca gcgatcttat tgttactgtc gaagaatccg atggctcaaa gcggacattc
     2701 tcgcaacctt tttcatcggt cgttcaaatg ttacgccctg gcgttggacg ttgggatatt
     2761 agcggcggcc ag
//
LOCUS       DAZFVO020000300         2733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFVO020000300 DAZFVO020000000
VERSION     DAZFVO020000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.302"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            59..289
                     /locus_tag="ACHKNB_RS25225"
                     /old_locus_tag="pgaptmp_005045"
     CDS             59..289
                     /locus_tag="ACHKNB_RS25225"
                     /old_locus_tag="pgaptmp_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198386.1"
                     /translation="MARNEVKPDWHPQRILGEVRAREGSMRALARKAGVGQNNLYNAV
                     YRHVPKYEGIIAECLGVPPEEIWPSRYIRKVA"
     gene            292..2379
                     /locus_tag="ACHKNB_RS25230"
                     /old_locus_tag="pgaptmp_005046"
     CDS             292..2379
                     /locus_tag="ACHKNB_RS25230"
                     /old_locus_tag="pgaptmp_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198387.1"
                     /translation="MFASVKELVGLPGMPGTEQGVRYSIRKYATSETSRRRKKGAKGF
                     EYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSAVVDAVEAYRGSPQL
                     MVERLNALTENQRQVADARIAIVSEVLKFTQQPGFSCAKAIRFLVNSLARSQLDERIV
                     AMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLP
                     EFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQ
                     KGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVID
                     GGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGIL
                     PRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRS
                     AFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYSP
                     AEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGR
                     KVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAE
                     EKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGLQ
                     LLEAKLKESDPLA"
     gene            2462..>2733
                     /locus_tag="ACHKNB_RS25235"
                     /old_locus_tag="pgaptmp_005047"
     CDS             2462..>2733
                     /locus_tag="ACHKNB_RS25235"
                     /old_locus_tag="pgaptmp_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198388.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVR"
BASE COUNT          725 a          660 c          787 g          561 t
ORIGIN      
        1 caaacttgat aactaagttt agttatcaaa tttcgtaact ttttaggata gatgaatcat
       61 ggcgagaaat gaagttaagc ctgattggca tcctcaaaga attttggggg aagtcagagc
      121 cagagaagga agcatgaggg cgctggcccg taaagctggc gtgggtcaga acaatctgta
      181 caacgcggta taccgccatg ttcccaagta tgaggggatt atcgcggaat gcctgggggt
      241 gccgcccgag gagatttggc ccagccgcta cattcgtaag gtcgcgtaat tatgtttgct
      301 tcagttaaag aactggttgg attaccgggg atgccgggaa cggagcaagg cgttcgctat
      361 tcaatcagga agtatgcaac cagcgaaacc agcagacgcc gcaagaaagg cgctaaaggt
      421 tttgaataca gcattgactg tttacctgaa gtgacacagc aggcattacg cgaacgttat
      481 gccctgcaac tgatgacgca aaaagccgat gaatcaccgg ctccggtggt gacaaaggcc
      541 agacgctcgt ctgccgtggt tgatgcggtg gaggcatatc gcggatcacc acaactgatg
      601 gtcgaacgcc tcaatgccct gactgaaaac cagcgccagg tggcagacgc acgcattgcg
      661 atcgtcagtg aggtgctgaa attcacgcaa caacccggtt tcagctgcgc taaagctatc
      721 cggttcctcg ttaacagcct ggcacgatca caactggacg agcgcattgt ggcaatggtt
      781 gagacggcga acgccaaaaa gggaaacagc cgcgcgttga gtgaaatcac gctgaaacgc
      841 tggattgcgg cctttaacaa ggcacagaac gccgctgaac gcctgctttt actggcaccg
      901 ggtaaacgcc aggaaataaa agccgaagat attaactggc tgcccgaatt tctggcgcag
      961 tatcgccagt caaacggccg accaatgacc gaggcttacg aggattttgt tgctgaatgg
     1021 cagcaccggc acgctgatga gccttatatg ctcgatatca tgccctctta tgacaccatt
     1081 cgccgcgcaa tgaagaaact gccggaagtg gtgaaacaaa aaggccgggt aactggcagt
     1141 gaataccgtc agcttgaggg attcacgcgc cgcgactggt ccagaatgcc ggtgaattat
     1201 gtctggattg gtgacggtca cggcatgaag ctgaaatgcg cgcacccggt tcacgggcgg
     1261 ccattcgcac cggaagtgac ctttgttatc gacggtggca cgcgctttgt ggtgggctgg
     1321 agccttgacc tggctgaaaa tgttttcgcc gtagccggtg ccatacagca cggtattcgc
     1381 catcatggca aaccgtttct gtattactcg gataatggct ccggggaaac cgccgacgtc
     1441 ctggataagg agattgtggg gatactgcca cgactgggga ttaatcaccc gacagggatt
     1501 gccggtaatc cgcagggacg gggcattatc gaacggctta accgcacatt accgatgcgc
     1561 atagcccgta aataccgcac ctatttcggg aaaggagcag atcgcgagac gttacgtaaa
     1621 accaaccgcg atttacgctc ggcattcagt gccctgcaac agggcaaacg gctgaacgcc
     1681 cggcagcagt cagcgatgcg tgatttaccg tcctggtctg aactgattga tgccattcgt
     1741 gacggtgttg agtggtacaa caaccggccg cacgatgaat tacccatgaa gccgaacggt
     1801 aaacattaca gcccggcgga gttcagaaaa aaacgcctgg cggaagagga cacggaaatt
     1861 gaatggctgt ccgatgttga actgcgggat atgttccggc cgatggtgga gcgccctgta
     1921 agacgctgtg aaatacgctg gctgaataat atttactacg cgcccgagct gcgtgacgag
     1981 catggccgta aggtacttat cagctatgac attcatgatg ccgaacgaat taccgtgcgc
     2041 cgcccggatg gcagcgtgat ttgcgaggcg gtatgggacg gcaataaacg cgaagccttc
     2101 cctgtcagcg cggaatacta caaacagcag cagcgcctta aaggcatgcg taaacgcgca
     2161 gaggaaaaaa tccgtgatgc cgaggatgag gttgtcaacg tgctggagca caagccgcag
     2221 gagccctggc tggaaaacat ataccgccct gtgggtaata cggtgaccgt tcagcaaccg
     2281 gtcgctgacg atgaacctga tgaagaatac gagcgtaatt tccagcgggg attgcaactg
     2341 ctcgaagcga aattaaaaga aagtgatccg ctggcctgaa ataaaaaaat aacccgaggg
     2401 gtgactcagg ttatttgagg tttcagtctc ggagaatgct gtctcaagcg aggtgaataa
     2461 tatgaccgat attaacgatg taatcaagac cattgatgaa cttattgatg gcggcgtact
     2521 gacgcagtat gccatcgcca gagaggcggg aatttccgac ggcacattat cggctttccg
     2581 taagggaaaa tataaaggcg ataacgctgc tgtggctgct tccctgcgct cctggtatga
     2641 gaactggaat aaacaaagcg cactgccgga accgccgcag tttgtggaaa ctcagacagt
     2701 ccaggagctg cgcgcactgt ttcaggcggt tcg
//
LOCUS       DAZFVO020000301         2733 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFVO020000301 DAZFVO020000000
VERSION     DAZFVO020000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.303"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            275..964
                     /gene="paoA"
                     /locus_tag="ACHKNB_RS25240"
                     /old_locus_tag="pgaptmp_005048"
     CDS             275..964
                     /gene="paoA"
                     /locus_tag="ACHKNB_RS25240"
                     /old_locus_tag="pgaptmp_005048"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HGU4198389.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            961..1917
                     /gene="paoB"
                     /locus_tag="ACHKNB_RS25245"
                     /old_locus_tag="pgaptmp_005049"
     CDS             961..1917
                     /gene="paoB"
                     /locus_tag="ACHKNB_RS25245"
                     /old_locus_tag="pgaptmp_005049"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HGU4198390.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            1914..>2733
                     /gene="paoC"
                     /locus_tag="ACHKNB_RS25250"
                     /old_locus_tag="pgaptmp_005050"
     CDS             1914..>2733
                     /gene="paoC"
                     /locus_tag="ACHKNB_RS25250"
                     /old_locus_tag="pgaptmp_005050"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HGU4198391.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRP"
BASE COUNT          628 a          800 c          761 g          544 t
ORIGIN      
        1 tttatattag aagtgacaga gatttgcagg gtgatgcaga gctgaaaatc acaggtttcc
       61 ttattggttt ttgcatcgta caactaaagc aataaaccag ctcccatcat catcaacatc
      121 ccggcgaaca tgaatttatt agccgggctc atagctgcat tcgcgtttta agaataatcc
      181 tcctgctgtc gccgactatg cttaacattt aaaaaagcat cagcactctc gcaacgcact
      241 cttattttcc cctttagaat accggaggcc tggtatgagc aaccaaggcg aataccccga
      301 agataatcgg gttgggaagc acgagccgca cgatttaagt ttgacccgtc gcgatctgat
      361 taaagtgagc gccgcaacag cggcgaccgc cgtggtttat cctcattcta cgctggcggc
      421 aagcgttccg gcagctacac ccgcgccaga gataatgccc ctgacactga aggtgaacgg
      481 caaaaccgag cagcttgagg tggatacccg aaccacgcta ctggacgctt tgcgtgaaaa
      541 tctgcatttg atcggtacca agaaaggttg cgatcacgga cagtgcggag cctgtaccgt
      601 gctggtcaat ggtcgcaggc ttaatgcctg cctgacgctt gcagtcatgc atcagggggc
      661 cgagatcacc accattgaag gcctgggttc gccagataat cttcacccca tgcaggcggc
      721 ctttatcaag catgatggct tccagtgcgg ctactgcacc tccgggcaaa tttgctcatc
      781 agtagcggcg ctaaaagaga ttcaggacgg cattcccagt cacgtcacgg tcgatttggt
      841 ttccgctcca gaaacaactg ccgatgagat ccgtgaacgt atgagcggca acatctgtcg
      901 ctgtggtgca tacgctaaca tccttgccgc cattgaagat gctgcggggg agataaaatc
      961 atgaaggcgt ttacctatga acgagtgaac accccagccg aggcggcact tagcgctcag
     1021 cgcgtacccg gcgcaaaatt tatcgcgggc gggaccaatc tgctggacct gatgaagctg
     1081 gaaattgaaa cgcccaccca ccttatcgat gtgaacggcc tcgggctcga taagattgaa
     1141 gtgaccgacg cgggtgggct gcgcatcggc gcactggtac ggaacaccga cctggcggct
     1201 cacgagcgcg tgcgtcgtga ttacgcggta ctctcccgcg ccctgctcgc tggcgcatct
     1261 ggtcagttac gtaatcaggc aaccaccgca ggtaatctgc tccagcgcac gcgctgcccc
     1321 tatttttacg acaccaatca gccctgcaat aagcgcctgc ccgggagcgg ctgcgcggcg
     1381 cttgaaggct ttagccgtca gcacgcggtg gtaggcgtaa gtgaagcctg catagccacc
     1441 catccgagcg atatggcggt cgcaatgcgg ttgctggatg cggtggtgga aaccatcacg
     1501 ccggagggaa agactcgcag tatcacactg gctgattttt atcacccacc ggggaaaacg
     1561 ccgcacattg aaaccgccct gcttcccggt gagcttatcg ttgcggtgac gttacctcca
     1621 ccgctcggcg gaaaacatat ctaccgtaag gtgcgcgatc gcgcctccta cgcctttgcc
     1681 ctggtatcgg tcgcggcgat tattcagcct gacggcagcg ggcgcgtcgc gctgggcgga
     1741 gtagcacata agccctggcg cattgaggct gcggatgctc agctatccca gggggcgcag
     1801 gccgtatatg acgcgctgtt cgccagcgcc catcccaccg ctgaaaatac ctttaaactc
     1861 ctgttggcga agcgaacgct tgcctccgta ctggctgaag cgagggcaca ggcatgaaat
     1921 ttgataaacc cgcaggggaa aacccgatcg atcagctgaa ggttgtcggt cgtccccatg
     1981 accgcatcga cggaccgctg aaaactaccg gcacggcacg ctacgcctac gaatggcatg
     2041 aagaagcccc caacgccgcc tatggctata tcgtcggttc cgccattgcc aaaggacgcc
     2101 tcaccgccct tgatacggac gccgcgcaaa aagcgccggg cgtactggct gtcattaccg
     2161 ccagtaacgc cggggcactc ggcaaaggcg acaaaaacac cgccagactg ttaggcggcc
     2221 ccactattga gcactatcat caggccattg cgctggtagt ggccgagacc ttcgaacagg
     2281 cgcgagcggc ggcctcgctg gtgcaggcac actatcgccg taataaagga gcttactccc
     2341 tggcggacga aaaacaggcc gtcaatcagc cgccggaaga cacgcccgac aaaaacgtcg
     2401 gtgactttga cggggctttc acctccgctg cggtgaagat tgatgctacc tacacgaccc
     2461 cggaccagag ccatatggcg atggagccgc atgcctcgat ggccgtctgg gatggaaata
     2521 agcttaccct ctggacctca aatcagatga ttgactggtg ccgcaccgat ctggcaaaaa
     2581 cgctgaaagt tcccgtggag aatgtgcgta ttatctcccc gtatatcggc ggagggtttg
     2641 gcggcaagct gttcctgaga agcgatgcgc tgctggcggc cctcgccgcc cgagcggtga
     2701 aacgtccggt taaagtgatg ctcccccgcc cct
//
LOCUS       DAZFVO020000302         2724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFVO020000302 DAZFVO020000000
VERSION     DAZFVO020000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.304"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            470..613
                     /locus_tag="ACHKNB_RS25255"
                     /old_locus_tag="pgaptmp_005051"
     CDS             470..613
                     /locus_tag="ACHKNB_RS25255"
                     /old_locus_tag="pgaptmp_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HGU4198392.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            626..835
                     /gene="cspE"
                     /locus_tag="ACHKNB_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             626..835
                     /gene="cspE"
                     /locus_tag="ACHKNB_RS25260"
                     /old_locus_tag="pgaptmp_005052"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HGU4198393.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1001..1810
                     /gene="rlmA"
                     /locus_tag="ACHKNB_RS25265"
                     /old_locus_tag="pgaptmp_005053"
     CDS             1001..1810
                     /gene="rlmA"
                     /locus_tag="ACHKNB_RS25265"
                     /old_locus_tag="pgaptmp_005053"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HGU4198394.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1807..2373)
                     /gene="mntP"
                     /locus_tag="ACHKNB_RS25270"
                     /old_locus_tag="pgaptmp_005054"
     CDS             complement(1807..2373)
                     /gene="mntP"
                     /locus_tag="ACHKNB_RS25270"
                     /old_locus_tag="pgaptmp_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HGU4198395.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          736 a          625 c          635 g          728 t
ORIGIN      
        1 gattaagcgt ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca
       61 tcaatttttc ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat
      121 tgatagccgt taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
      181 agcaaaaaat gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac
      241 tgaacttatt gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag
      301 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
      361 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
      421 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
      481 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
      541 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
      601 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
      661 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagatg tgttcgtaca
      721 cttctccgct atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt
      781 cgaaattcag gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
      841 tccactgatc tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag
      901 gctttttact atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc
      961 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact
     1021 ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
     1081 tgatatggcg aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga
     1141 tccgggcgac agcgcggaaa tgatacaagc acgccgcgca ttcttagatg ccggacatta
     1201 tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac
     1261 ggcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgtgtt
     1321 gcccgaaatc accacgtttg gcctggatgt ctcgaaggta gcgataaaag cggcggcgaa
     1381 acgctatccg caggtcactc tttgtgtcgc ttccagccac cgtttgccgt tttccgatac
     1441 cagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt
     1501 agtgaagccc ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct
     1561 gaaggggctg atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg
     1621 ttttacatta cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc
     1681 cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac
     1741 actggcagca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
     1801 ttcttattaa ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagcaccag
     1861 cccgccgaga atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat
     1921 ccctaatgtc gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt
     1981 gacctgcagg aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc
     2041 ggtggttacc agtagccaga aaccgtgccg acggcgcggc tcttcatctt catcatctgc
     2101 gccacgaaaa ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat
     2161 ccagtgattc cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag
     2221 cggcgtcagg gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt
     2281 cggtttatgg agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc
     2341 aaacgcaaga agaacagtag cagtgatatt catgacaacg tcctgaccgg ggtatccata
     2401 aaacacatca tcgcccccag caaacatgca tacatgaaca acaagccgcg atggttttcg
     2461 cgttcatgca ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt
     2521 ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat
     2581 cggctactcc ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt
     2641 ggaatatatt atttcattgc tttattgata acgatttaca tttagattta tatatcaatg
     2701 aaacgttaaa ataaataaca aata
//
LOCUS       DAZFVO020000303         2683 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFVO020000303 DAZFVO020000000
VERSION     DAZFVO020000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.305"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            87..332
                     /locus_tag="ACHKNB_RS25275"
                     /old_locus_tag="pgaptmp_005055"
     CDS             87..332
                     /locus_tag="ACHKNB_RS25275"
                     /old_locus_tag="pgaptmp_005055"
                     /inference="COORDINATES: protein motif:HMM:NF025073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198396.1"
                     /translation="MSLIEDWSSADIKAALEKRDTNLSRLSRENNLQPRTLGNALRVP
                     YPKGEKIIARAIGVEPQVIWPTRYERRARRKYVGCND"
     gene            313..2397
                     /locus_tag="ACHKNB_RS25280"
                     /old_locus_tag="pgaptmp_005056"
     CDS             313..2397
                     /locus_tag="ACHKNB_RS25280"
                     /old_locus_tag="pgaptmp_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198397.1"
                     /translation="MWVAMTELISFSDLPSSLAGLHKKAKREAWKTRLKPGVKGKVLE
                     CEIGALPLTVQQAVRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQLM
                     VERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERIVA
                     MVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWLPE
                     FLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVKQK
                     GRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVIDG
                     GTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGILP
                     RLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLRSA
                     FTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYSPA
                     EFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGRK
                     VLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAEE
                     KIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGLQL
                     LEAKLKENDPLA"
     gene            2468..>2683
                     /locus_tag="ACHKNB_RS25285"
                     /old_locus_tag="pgaptmp_005057"
     CDS             2468..>2683
                     /locus_tag="ACHKNB_RS25285"
                     /old_locus_tag="pgaptmp_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198398.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPP"
BASE COUNT          734 a          627 c          758 g          564 t
ORIGIN      
        1 atatcaaaca tacactagtg agtaactcaa tagagtagca cacatgagta ataaagtttg
       61 ccatagaaag tacacagggg gccattgtga gtcttattga agattggtct tccgccgata
      121 taaaggcagc cttagaaaaa cgcgatacca atttgtcacg tttatcaagg gaaaataatt
      181 tgcagcctag gactttgggt aatgctttgc gagttcccta ccctaaaggt gaaaagataa
      241 ttgctagggc tataggtgta gagccacagg ttatttggcc tactagatat gaacgacgtg
      301 cgcggaggaa atatgtgggt tgcaatgact gaattgattt cattcagtga cctacctagt
      361 tctttggcag gtctgcacaa aaaagcaaag agggaagcgt ggaaaacgcg gctaaaacca
      421 ggggtaaaag ggaaagtttt agagtgtgaa ataggagctt tgcctttaac tgttcagcag
      481 gcagtacgcg aacgctatgc cctgcaactg atgacgcaaa aagccgatga atcaccggct
      541 ccggtagtga caaaggccag gcgctcacct gccgtggttg atgcggtgga ggcatatcgc
      601 ggatcaccac aactgatggt cgaacgcctt aatgccctga ctgaaaacca gcgccaggtg
      661 gctgatgcac gaatcgcgat cgtcagcgaa gttctgaaag tcgcgcaaca acctggtttc
      721 agctgtgcga aggctatccg gtttatcgtt gacaacctgg cacgttcgca gctggacgag
      781 cgcattgtgg caatggttga gacggcgaac gccaaaaagg gaaacagccg cgcgttaagt
      841 gaaatcacgc tgaaacgctg gattgcggcc tttaacaagg cgcagaacgc cgctgaacgc
      901 ctgcttttac tggcaccggg taagcgccag gaaataaaag ccgaagatat taactggctg
      961 cccgaatttc tggcgcagta tcgccagtca aacggccgac caatgaccga ggcttacgag
     1021 gattttgtcg ctgaatggca gcaccggcac gctgatgagc cttatatgct cgatatcatg
     1081 ccgtcttatg acaccattcg ccgcgcaatg aagaaactgc cggaagtggt gaaacaaaaa
     1141 ggccgggtga ccggcagtga ataccgccag cttgagggat tcacgcgccg cgactggtca
     1201 aaaatgccgg tgaattatgt ctggattggt gacggtcacg gcatgaagct gaaatgcgca
     1261 cacccggttc acggtcggcc atttgcaccg gaagtgacct ttgttatcga cggtggcacg
     1321 cgctttgtgg tgggctggag ccttgacctg gctgaaaatg ttttcgccgt agccggtgcc
     1381 atacagcacg gcattcgcca tcacggcaaa ccgtttctgt attactcgga taacggctcc
     1441 ggggaaaccg ccgacatcct ggataaggag gttgtgggga tactgccgcg actggggatt
     1501 aatcacccga ccgggattgc cggtaatccg cagggacgag gcattatcga acggcttaac
     1561 cgcacattac cgatgcgcat agcccgtaaa taccgcacct atttcgggaa aggtgcagat
     1621 cgcgagacgt tacgcaaaac caaccgcgat ttacgctcgg catttactgc cctgcaacag
     1681 ggcaaacggc tgaacgcccg gcagcagtca gcgatgcgtg atttaccgtc ctggtctgaa
     1741 ctgattgatg ccattcgtga tggtgttgag tggtacaaca accggccgca cgatgaatta
     1801 ccggtgaagc cgaacggcaa gcattacagc ccggcggagt tcagaaaaaa acgcctggcg
     1861 gaagaggaca cggaaattga atggctgtcc gatgtggaat tgcgggacat gttccggccg
     1921 atggtggaac gccctgtaag acgctgtgaa atacgctggc tgaataatat ttactacgcg
     1981 cccgagctgc gtgatgagca tggccgcaag gtgcttatca gctatgacat tcatgatgcc
     2041 gaacgaatta ccgtacgtcg cctggatggc agcgtgattt gcgaggcggt atgggacggt
     2101 aataaacgcg aagccttccc ggttagcgcg gaatactaca aacagcagca acgccttaaa
     2161 ggcatgcgta aacgcgcaga ggaaaaaatc cgtgatgccg aggatgaggt tgtcaacgtg
     2221 ctggagcaca agccgcagga gccctggctg gaaaacatct atcgcccggt aggtaatacg
     2281 gtggccgttc agcaacctgc cgttgatgat gagcctgatg aagaatacga gcgcaatttc
     2341 cagcggggat tgcagttgct cgaagcgaaa ttaaaagaaa atgacccgct ggcctgaaat
     2401 aaaaaataac ccgagcggcg actcaggtta tttgattaaa caaggtatca aatgagaggt
     2461 taataatatg actgatatta acgatgtaat caagaccatt gatgaactta ttgatggcgg
     2521 cgtactgacg cagtatgcca tcgccagaga ggcgggaatt tccgacggca cattatcggc
     2581 tttccgtaag ggaaaatata aaggcgataa cgctgctgtg gctgcttccc tgcgttcctg
     2641 gtatgagaac tggaataaac aaagcgcact gccggaaccg ccg
//
LOCUS       DAZFVO020000304         2578 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFVO020000304 DAZFVO020000000
VERSION     DAZFVO020000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.306"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2566
                     /locus_tag="ACHKNB_RS25290"
                     /old_locus_tag="pgaptmp_005058"
     rRNA            <1..2566
                     /locus_tag="ACHKNB_RS25290"
                     /old_locus_tag="pgaptmp_005058"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          671 a          568 c          811 g          528 t
ORIGIN      
        1 gtacacaaaa atgcacatgc tgtgagctcg atgagtaggg cgggacacgt ggtatcctgt
       61 ctgaatatgg ggggaccatc ctccaaggct aaatactcct gactgaccga tagtgaacca
      121 gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga gtgaaaaaga acctgaaacc
      181 gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac tgcgtacctt ttgtataatg
      241 ggtcagcgac ttatattctg tagcaaggtt aaccgaatag gggagccgaa gggaaaccga
      301 gtcttaactg ggcgttaagt tgcagggtat agacccgaaa cccggtgatc tagccatggg
      361 caggttgaag gttgggtaac actaactgga ggaccgaacc gactaatgtt gaaaaattag
      421 cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg ggagatagct ggttctcccc
      481 gaaagctatt taggtagcgc ctcgtgaatt catctccggg ggtagagcac tgtttcggca
      541 agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga atgttatcac
      601 gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc agaccgccag
      661 ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag acagccagga
      721 tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg tcgagtcggc
      781 ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc gacactgtgt
      841 gttgttgggt aggggagcgt tctgtaagcc tgcgaaggtg tgctgtgagg catgctggag
      901 gtatcagaag tgcgaatgct gacataagta acgataaagc gggtgaaaag cccgctcgcc
      961 ggaagaccaa gggttcctgt ccaacgttaa tcggggcagg gtgagtcgac ccctaaggcg
     1021 aggccgaaag gcgtagtcga tgggaaacag gttaatattc ctgtacttgg tgttactgcg
     1081 aaggggggac ggagaaggct atgttggccg ggcgacggtt gtcccggttt aagcgtgtag
     1141 gctggttttc caggcaaatc cggaaaatca aggctgaggc gtgatgacga ggcactacgg
     1201 tgctgaagca acaaatgccc tgcttccagg aaaagcctct aagcatcagg taacatcaaa
     1261 tcgtacccca aaccgacaca ggtggtcagg tagagaatac caaggcgctt gagagaactc
     1321 gggtgaagga actaggcaaa atggtgccgt aacttcggga gaaggcacgc tgatatgtag
     1381 gtgaagtccc tcgcggatgg agctgaaatc agtcgaagat accagctggc tgcaactgtt
     1441 tattaaaaac acagcactgt gcaaacacga aagtggacgt atacggtgtg acgcctgccc
     1501 ggtgccggaa ggttaattga tggggtcagc gcaagcgaag ctcttgatcg aagccccggt
     1561 aaacggcggc cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc
     1621 gacctgcacg aatggcgtaa tgatggccag gctgtctcca cccgagactc agtgaaattg
     1681 aactcgctgt gaagatgcag tgtacccgcg gcaagacgga aagaccccgt gaacctttac
     1741 tatagcttga cactgaacat tgagccttga tgtgtaggat aggtgggagg ctttgaagtg
     1801 tggacgccag tctgcatgga gccgaccttg aaataccacc ctttaatgtt tgatgttcta
     1861 acgttgaccc gtgatccggg ttgcggacag tgtctggtgg gtagtttgac tggggcggtc
     1921 tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa tcctggtcgg acatcaggag
     1981 gttagtgcaa tggcataagc cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa
     2041 gcaggtcata gtgatccggt ggttctgaat ggaagggcca tcgctcaacg gataaaaggt
     2101 actccgggga taacaggctg ataccgccca agagttcata tcgacggcgg tgtttggcac
     2161 ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc ccaagggtat ggctgttcgc
     2221 catttaaagt ggtacgcgag ctgggtttag aacgtcgtga gacagttcgg tccctatctg
     2281 ccgtgggcgc tggagaactg aggggggctg ctcctagtac gagaggaccg gagtggacgc
     2341 atcactggtg ttcgggttgt catgccaatg gcactgcccg gtagctaaat gcggaagaga
     2401 taagtgctga aagcatctaa gcacgaaact tgccccgaga tgagttctcc ctgactcctt
     2461 gagagtcctg aaggaacgtt gaagacgacg acgttgatag gccgggtgtg taagcgcagc
     2521 gatgcgttga gctaaccggt actaatgaac cgtgaggctt aaccttacaa cgccgaag
//
LOCUS       DAZFVO020000305         2577 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFVO020000305 DAZFVO020000000
VERSION     DAZFVO020000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.307"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            215..562
                     /locus_tag="ACHKNB_RS25295"
                     /old_locus_tag="pgaptmp_005059"
     CDS             215..562
                     /locus_tag="ACHKNB_RS25295"
                     /old_locus_tag="pgaptmp_005059"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198399.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            735..899
                     /locus_tag="ACHKNB_RS25300"
                     /old_locus_tag="pgaptmp_005060"
     CDS             735..899
                     /locus_tag="ACHKNB_RS25300"
                     /old_locus_tag="pgaptmp_005060"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198400.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(903..1091)
                     /locus_tag="ACHKNB_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /pseudo
     CDS             complement(903..1091)
                     /locus_tag="ACHKNB_RS25305"
                     /old_locus_tag="pgaptmp_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1152..2276
                     /locus_tag="ACHKNB_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /pseudo
     CDS             1152..2276
                     /locus_tag="ACHKNB_RS25310"
                     /old_locus_tag="pgaptmp_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
     gene            complement(2273..>2577)
                     /locus_tag="ACHKNB_RS25315"
                     /old_locus_tag="pgaptmp_005063"
     CDS             complement(2273..>2577)
                     /locus_tag="ACHKNB_RS25315"
                     /old_locus_tag="pgaptmp_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HGU4198401.1"
                     /translation="HHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWG
                     TLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          714 a          570 c          628 g          665 t
ORIGIN      
        1 gcggtatgtt catcttgagc aattagaata taccgaatta agaaatgctt tagcttgtgc
       61 attgtcaact tgttataata gaaaatatct cattaaagtt gcaaaaggaa aatatttacc
      121 tgttgacgac taggtgcgta ttaccgtgaa ggagatcggt gagtaacccc ctgtgtcagc
      181 atagttgtcg cctcaatttt ttaaggagat aacaatgcca ggacaaccaa taccaagtga
      241 gctcaaagag aaaggactga tatggctttt accaccatac tgctggtcac atcgtcagat
      301 agcccgtaag ctaaagatca gtgcctcagt tgtatcaaaa tggagatatg agttagtgga
      361 gcaagggctt ttacctgaaa atgaaaagat tacctccgtt gatgttgaag actggacacc
      421 ggagcgtcga ttctcagttg ttcttgagac ggctacgctg tcagaaatgg agcttgctga
      481 ttattaccga cgcatgggat tgtttgttga acaggttaaa gaatggcggg ctatctcaat
      541 tcaggcgcat gaaaaaaatt gacggaaaat attcgaacag acagggcatt gcgtgacgca
      601 aaaaataagt tacgagaact gtagaaagag cttaagcgta aagataaagc tctggcggaa
      661 gctgcggccc tgcttatatt gagggaaaag ttcaatgccc tctgggacaa cagcgaggaa
      721 gactgacccc tctcctggag cggcgtaaaa ttattgccct tgtccgcgag gccgtctcca
      781 acggcgcccg gaaaagtcag gcttgtaggt gtatcggtat ttcaattcgg acacttcagc
      841 gctggactga tggcaacgtt attaccacgg atgtaagcgt caacggagca ccgtattgac
      901 gcttatttat tggtgagtac tacgttccat ggcaggagtt catcaactcg gttggaaggc
      961 cattccggca gtacgctcag gataaggcgc agatacgctt ccggatcgat accgttcaga
     1021 cggcaggtgc cgatcagccc gtacaacagt gctccacgct cgccgccgtg atcgctgcca
     1081 aagaacataa agatggtatg acctttatat actcactata gcattggtgg gatattccag
     1141 aggaggtcat tatgcattcg gaaaatattg cagcttacat cgggctggat gtacataaag
     1201 aaacgttggc ggttgctatt gctgcacctg aacggcttgg cgaagttcgt tattatggta
     1261 cgattaacaa cgaagcccag gctgtacgtc gattatttca gaaattgcag ggactttacg
     1321 gaaacattct ttcctgctat gaggctggcc cctgtggttt tgggctatat catcaactca
     1381 cagcgatgaa tataaaatgc caggttattg ccccttcccg gataccaaaa tcaccgacag
     1441 acagaatcaa aaatgatcat cgggacgcaa tttctcttgc caggttactg cgggctggtg
     1501 aacttacccc tgtctggatc cctgacctga ctcatgaagc tatgcgggat ctaatcagag
     1561 cccgtgcagc agctaaacga gacagcagag ttgcccgaca gcgaattctg agcatgctat
     1621 tgcgaacaga caaacgttat gcagggaagc actggaccgg caaacatcga acctggttag
     1681 ccaatcaatc atttagtcag ccatctcaac agattgcttt tcaacactat tgtcagagtc
     1741 tggagcagat tgaagatcgc atactttaac tcgaccagga aatatcccgc ctgttacctg
     1801 agtggtctct gtgcaatctg gtctgccagt tacaggccct taaaggtgtt ggtcagctaa
     1861 ccgcaataac cctggttgct gaactgggtg atttttcacg attttccagt cccaaacaac
     1921 tcatggcttt tctgggactg gttccagggg aatattccag tggaaacagc attcgtccca
     1981 ggggaataac aaaagttgga aacagcgaac tgagacgtct gctttacgaa gccgcctggt
     2041 cttatcgtac acctgcaaaa gttggtgcat ggcttatata ttaccgaccg gattctgtaa
     2101 cacaatattc caaagatatt gcatggaaag ctcaacaacg attgtgttct cgttaccgaa
     2161 gtctgacagc aaaagggaaa aaatcacaag tagccattac ggcggtggct cgtgagttga
     2221 ctggatttat gaggtgtact ggcaatagcg gacactacca tttgttcttt ttttaagcag
     2281 ccatctgatg atatttttcc ctgaaggctg ccggggagat attccccaga cgagagtgac
     2341 gacgctgacg attgtagaaa atctcaatgt attcccgtat tactgagatg gcttcatccc
     2401 ggttattaaa acgatagtgg ctcaggctct catttttcag cgttccccag aagctttcca
     2461 tcggagcgtt gtcgtaacag ttacctttac gcgacattga tgttttcaga ccaaactgct
     2521 cctgtatgac ccggtaatcg tatgcgcagt actgtgaacc tcgatcagag tggtgga
//
LOCUS       DAZFVO020000306         2529 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFVO020000306 DAZFVO020000000
VERSION     DAZFVO020000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.308"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..185
                     /locus_tag="ACHKNB_RS25320"
                     /old_locus_tag="pgaptmp_005064"
     CDS             <1..185
                     /locus_tag="ACHKNB_RS25320"
                     /old_locus_tag="pgaptmp_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198402.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDSVVREALLRDFDNPPFKKV
                     PAYWLHPGLTYLKVRI"
     gene            222..755
                     /locus_tag="ACHKNB_RS25325"
                     /old_locus_tag="pgaptmp_005065"
     CDS             222..755
                     /locus_tag="ACHKNB_RS25325"
                     /old_locus_tag="pgaptmp_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4198403.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEALRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            912..1094
                     /locus_tag="ACHKNB_RS25330"
                     /old_locus_tag="pgaptmp_005066"
     CDS             912..1094
                     /locus_tag="ACHKNB_RS25330"
                     /old_locus_tag="pgaptmp_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198404.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1109..1240
                     /locus_tag="ACHKNB_RS25335"
                     /old_locus_tag="pgaptmp_005067"
     CDS             1109..1240
                     /locus_tag="ACHKNB_RS25335"
                     /old_locus_tag="pgaptmp_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198405.1"
                     /translation="MSDKLIMLAKILCVIIGISFSLMLVALFLSMGWMMLSSSGMLG"
     gene            1463..1669
                     /locus_tag="ACHKNB_RS25340"
                     /old_locus_tag="pgaptmp_005068"
     CDS             1463..1669
                     /locus_tag="ACHKNB_RS25340"
                     /old_locus_tag="pgaptmp_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HGU4198406.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            1734..1958
                     /locus_tag="ACHKNB_RS25345"
                     /old_locus_tag="pgaptmp_005069"
     CDS             1734..1958
                     /locus_tag="ACHKNB_RS25345"
                     /old_locus_tag="pgaptmp_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198407.1"
                     /translation="MQKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVEYTMNAAKCRMVSAALQPVC"
     gene            2309..>2529
                     /locus_tag="ACHKNB_RS25350"
                     /old_locus_tag="pgaptmp_005070"
     CDS             2309..>2529
                     /locus_tag="ACHKNB_RS25350"
                     /old_locus_tag="pgaptmp_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HGU4198408.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHL"
BASE COUNT          627 a          548 c          707 g          647 t
ORIGIN      
        1 agcgcattgg tcgttgcatg gggtatctgc aggtagccgc tgagattgaa gcgctggcca
       61 gtggtgcgga caaggattca gttgtgcggg aggcacttct tcgtgatttt gataatccgc
      121 cctttaaaaa agtgccggct tactggcttc atcccggact gacttatctg aaagtgcgta
      181 tttagtgggc ctgggacagc ggctgaatat ttaatatatc catgaacaca aaaataaaat
      241 acggcctgtc ggctgccgtt ctggcgttga ttgccgcagg ggcttctgcg cctgaaatcc
      301 tcgaccagtt tcttgacgaa aaagaaggta accacactac ggcataccgt gatggtgcgg
      361 gtatctggac catctgccgt ggagccaccc gggtggatgg taagcctgtt attcctggca
      421 tgaagctgtc gaaggggaaa tgcgaccggg ttaacgccat tgagcgtgat aaggcgctgg
      481 catgggtgga gaaaaacatc aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt
      541 cattctgtcc ttacaacatt ggcccgggta agtgtttccc gtcgacgttt tataaacgaa
      601 ttaatgcagg agatcgcagg ggagcgtgtg aggcgcttcg ctggtggatt aaggacggtg
      661 gcagagactg ccgtatccgc tcaaataact gttatggtca ggtatcccgt cgtgaccagg
      721 agagcgcgct ggcgtgctgg ggtatcgaca gataagcaga atattttgct gaaaaatgac
      781 gttggccaac gcggacggat aacacgaaat cctgcgaact ggcaaaacct aagtgaataa
      841 aagtaaaaac cccgtttgtt ggcagcaagc ggggttttgt gtttctgacc ttggataagg
      901 caagggagaa catggaaaag tataaacgaa ttctgttgag gttgactatg aaaaacggcc
      961 ttgaactgaa agcgccagta actgatgaca tcagcagagc actggctttt gctattaagt
     1021 gggtggcggt cggtatcgct gtgtctccga tgctgtatgg gctggcaaaa ctggtcattg
     1081 cgttgaaatc gtgaagggag gattaaacat gtcagacaaa ctcataatgc tggcgaagat
     1141 cctctgtgta atcatcggca tttcattttc actaatgctg gttgctcttt ttctttccat
     1201 gggctggatg atgttatctt cgtcggggat gctggggtga gcataaaccg aatgctttcc
     1261 gcgtttaccg ttattctgct ggtggtctgt ggtgcgctta gtctggggct gaatcattac
     1321 cgcgataacg ccatcaccta caaagcgcag cgtgataaaa aagccagaga gctggagctg
     1381 gcaaacgcaa ccattactga tatgcaggtg cgccagcgcg atgttgctgc gctcgatgca
     1441 aaatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt
     1501 gccgctggtc gtaagcgcct gcggatcaac gccacctgcc ccggtaccgt gcgtgaagcc
     1561 accggcacct ccggcgtggg caatgatgcc gccgtcgaac tctctccggt tgctggacga
     1621 aacgttctcg gtatcagaga cggaatcatc agcgaccagg cagcattgag aatgcttcag
     1681 gaatatatcc gcactcagtg tattaactag tatttttgtt attcggagaa tgcatgcaga
     1741 aattattggt aaccgtaaag ccttttcagg gaacaattcc gttccgtatt ttgcagcgtg
     1801 gtcgtgttct tgttgaaggt tcgttcagtg gtaaatgtac gcaattacac tcccggacct
     1861 ttcaggtgaa tgccacgaat gaagagctaa ccgttgagta tacgatgaat gccgctaaat
     1921 gccgcatggt atccgctgca ttacagccag tgtgttgagc gaccttatta ttcatgcgcg
     1981 gtattgtcgc cgtattcctg cattaacaga gacagcagcc cgaccgggag actcctctgc
     2041 gcgagtgtgc ggggataatc aaaaacgata cacaccgggg tttaccgcgt taacggagcg
     2101 cggcgttgtc ccctcatggt cgctggtccg gtgcgatggt ggaagaaacc ggacgatgtg
     2161 ttacctcgca agacctgtta tgtcatgtgt ctgatttgtg atttaagtcg gataattgtc
     2221 gttgccatta agcagaggat tgatggccga cagggtggca ttgttagaat aagacttatt
     2281 cttatctgtg ccgggaatga aaatgaaaat gaaaagaaat cttccgttaa ttattttgtt
     2341 gtcttctctg gttatgggct gtacgcaaca taaaacagat atgccccgac agttggttaa
     2401 ggcattacca caatatccgg cctatgcagc ggcaaattat ataaagggac gggttgatgt
     2461 gaagtttgat attggtgctg atggtactgt cacccgaatt gagtttatcc gttcagagcc
     2521 gcaccatct
//
LOCUS       DAZFVO020000307         2497 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFVO020000307 DAZFVO020000000
VERSION     DAZFVO020000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.309"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(151..2418)
                     /gene="adiA"
                     /locus_tag="ACHKNB_RS25355"
                     /old_locus_tag="pgaptmp_005071"
     CDS             complement(151..2418)
                     /gene="adiA"
                     /locus_tag="ACHKNB_RS25355"
                     /old_locus_tag="pgaptmp_005071"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HGU4198409.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          574 a          615 c          683 g          625 t
ORIGIN      
        1 acaggcaaaa aaaagcgcgg ggtggggccg cgcaaaaagt atgacatttt tgtttgcagc
       61 aatttttatg aaaacgttgc catactcaaa aggcctaagg ccaggcggcg taatgttatt
      121 taaacaatta cgccttcagc ggaatagtgg ttacgctttc acgcacataa cgtggtaaat
      181 accgtcaata atttcagtcc cttcagtttc gtgttcaaat ccagggaaat ggtggtccca
      241 ggattgcagc gagcgtaaat aacttacttg cggactgttt ttatcgccga agttttcacc
      301 agacagcagc atcgggattc ctggcggata cgggataact gagtttgccg cgatgcgtcc
      361 tggcagattt tcaatggata ccagttcgac attgttgtcg acaatcgcgt tgtacgcttc
      421 acgcggggtg acttccgcca ccggcaggcc ggaataggct tcgttcaacc gtgcgccagg
      481 gttgttttct ttcagccagg caaacatggt gtcacccaga tcgtgaatcc ccatgttcgc
      541 gtaagtgtca ggatattgtt caacaagttc cggcatcacc tgcgccagcg gtgtgttggc
      601 gtcatagtgg cgtttgaagg agcaaagggt gttaaccaga gttccccatt tcccacgggt
      661 tacgcccata gagaacagga acataatttg gaagtcagtg gtgcgggtag gtacaatgcc
      721 gtggcgacca agccaggcag tgaccagcgc cgccggaaca ccggtttctt ccagttcacc
      781 atcttcaccc attcccggag caaggatgct gactttaatc gggtcgagca tactccagtt
      841 atccggaata tctttgaagc cgtgccagct ttcgcccgga tgcattaccc agcagtcctg
      901 aacggtggtc agcagtttgg ttggtgcgtc agcaaagtca taggttttgc cggtttgtgg
      961 gtcggtgacg acttctttgt tccacggttt gaagaaccag ctaccgtcag cggtgaactc
     1021 tttatacagc cgcgccatcg cctgacgaaa atcaaccgct tcgtcaatca cttcttgtgt
     1081 cagtgacagg ccgctgttgc cgtccatcat cgacaccgcc acgtcgttgg atgcgcagat
     1141 ggcatacagc ggggaggtgg tggcatgcat catgtaggcc tggttgaagc gggagaagtt
     1201 aatcgcccca cgaccttcac gtacatgaat ataagaagcc tgtgacagcg cattcagcag
     1261 tttgtgggtg gagtgggtgg cgaaaacggt aggaccgttg tgatcgccag gttcgccgcg
     1321 catggcatag tgatcggcat agatcgggtt gaaacgtgca tagccgtacc aggcttcgtc
     1381 aaagtgcaga cgatcggagg ttttttccag cagatcctgc gcttctttag cgttataaca
     1441 cacgccgtca taggtgcagt tggtcaccac gcagtaagac ggtttttgcc cagctttgtc
     1501 tttagtcagc gggctttcac tgattttctt ctgcaaggtt tcaggttgca tttcctgcgg
     1561 atagattggc ccgataatgc cgtagcggtt gcggcttggc accatgtaga ctggtttcgc
     1621 gcctgtcagc atcaagcctt gttcgatgga tttatggcaa ttacggtcaa cgaccacgac
     1681 atcgttatcg gtcatgcaag cctgcatgat ggtgcggtta gagccggaag taccgacgac
     1741 taccgaccag gagcgatcgg caccaaatac gcgtgcggca tatttttcgc tttcgccaaa
     1801 tgcgccagta tggtcaagca aagaaccgag ggaagttcgt tcgatgccca tgtcggtgcg
     1861 aaacagattt tcaccatagt agtcatggta gaaacgtccg gcgggtgttt tggtaaaacc
     1921 aacgccgccc tggtggcctg gcgctgccca ggaatattca tggatgtcac tatatttcat
     1981 cagcgcgctg aacagtggcg gcaacagctg ctggcggtag cgggtcatcg cggcaacggc
     2041 gcgtccggcg ataaagtcgg cggtatcttc cagaatccag gcgaattcat cgacaagctc
     2101 cagcagatcc cggtccattg cggcgagggc tttttcccga tcgcccaaca ggaagaccgg
     2161 cacgttttgt tggcgctcat gaagcttacc gatcaattgt ctgacgtttt gatgttcgtc
     2221 cggatgttcc atttgatagc tgaacatcag gcagtcaatg gcttcgtttg aagagagaat
     2281 ggcaaaacca tcatcaaagg aggtggattt aatcacggta acattttgct ggcttaaagc
     2341 atctgccaga cgctcaacgg cgttaccgac ccaggtgtct tgatggagaa actcgctttc
     2401 aacaattaat actttcatca ttgcttaccc ggttatgaag gaatcttcgt tgcgggcaag
     2461 tatcttccgg ccttttcggg ctgtaaagcg cgtgaaa
//
LOCUS       DAZFVO020000308         2464 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFVO020000308 DAZFVO020000000
VERSION     DAZFVO020000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.310"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..213
                     /locus_tag="ACHKNB_RS25360"
                     /old_locus_tag="pgaptmp_005072"
     CDS             <1..213
                     /locus_tag="ACHKNB_RS25360"
                     /old_locus_tag="pgaptmp_005072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198410.1"
                     /translation="KKMMLHSILFTQILKIIVCYLINNAELIDVFVVCRLWIIGGIKI
                     RSSWLYNPLPVSDTECVQVTGSGRAR"
     gene            1242..1787
                     /locus_tag="ACHKNB_RS25365"
                     /old_locus_tag="pgaptmp_005073"
     CDS             1242..1787
                     /locus_tag="ACHKNB_RS25365"
                     /old_locus_tag="pgaptmp_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HGU4198411.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMI
                     NQSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWTVQNKTN
                     NDLVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVF
                     NGDRVAFTTVLEMNKSSHKDK"
     gene            complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS25370"
                     /old_locus_tag="pgaptmp_005074"
     CDS             complement(1867..2292)
                     /gene="mobA"
                     /locus_tag="ACHKNB_RS25370"
                     /old_locus_tag="pgaptmp_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HGU4198412.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEY
                     IRTASLNNEIKQRVPSRYLDELIQLGRMQKKLFDNGKRPKDKEYLDVMHKIISLCAEM
                     KEVTQRISDIYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
BASE COUNT          727 a          487 c          501 g          749 t
ORIGIN      
        1 aaaaaaatga tgcttcattc aatattattt acacagattt taaaaattat tgtttgttat
       61 ttaataaata acgccgaatt aattgatgtc tttgttgtct gtcgtttgtg gattattggt
      121 ggaataaaga ttagatcttc ctggttatat aatcctttgc ctgttagtga tacggagtgt
      181 gtccaagtaa caggaagcgg aagagcaaga tagtagcatg tacataaaat ataaatacgt
      241 gagttattga tatcgcgttt atatattaaa aatataactt aacattgctc ttaatattta
      301 ttttgtcctg acaatgggag cattaatccc cgtgcagtgc tccgccagct gctgacaacc
      361 tcgacatata accatcgata cgatagaaca tcagggcgcg tttccggcaa gaactaacca
      421 tggggatggt ttgcgtgcca acgaacgccg gaaacgcgct tcaaacctac cgagtcgcca
      481 cggttccggc agcgccttga gcgcaaccgg aaaccgtctg gggagagccc cagcccccca
      541 ccgctgcaac atcgacggtc tatgaccacc caccaaccga gtagctgcct gtaccgtgca
      601 aatgggcttt gcagggtgct ggcgcggttc ctggcgacct gaaagcccct ggttaggggt
      661 atcggcgcgc ctggcctcct gaaaccctct gtaagcgttt ttcgcgcctt ccatgccctg
      721 gcgggcattt aaaacccgtt tcgtgcgtct gagagcttct gagagcgttt taggggctat
      781 ctggcaactc atccccgaaa gagcaaccgc ggaatcaccg acaacatgcg agaggtaatt
      841 ccgctcgccg caagtcgatg accgagcgta gcgagcgaat cgacgaggaa gcggaagaga
      901 accggaagct acattgagca cttacgcaca gaatttggcc tacgcacccg cgtccgtgca
      961 cgcgtttaat aagaattaag aagggaaatc gcgataaacc gcgccaatcc tagaatctaa
     1021 tgatcaggat attgatctaa cgtatcagaa tagagcatct aacatatcaa ttagttgata
     1081 tttaaagtat tgattgtttg ttttttgctc atcgtgagtg tgatttattt gatctttaag
     1141 gtatcaaaac tatgatacta gtcggatcgg tttattgaca tttgtagatt ttttcttgat
     1201 agtcgaggta tcagtttatt gatacctgag agtgtaaaat aatgagcaag agcaaggctt
     1261 taaacataaa caaacgattt agtgaaaatc ctttcatcaa agatttgata attcctgtaa
     1321 aaaacaaaca agtaagaata tcgcgattag gagaagataa taacataatg attaaccaat
     1381 caactggaga ggttttaggg ggaacctcgg tagtaactta taaaaaagtt gatagtgata
     1441 attttattaa attgtttacc caaaatataa aattggcttt cgagttaaca caagctggtc
     1501 aaaaagcact atttgtttta ttttggacag tacaaaataa aacaaataat gatctagtct
     1561 tattagatca atacacgcta aatgatttta taaatgaaaa caaagggtta agcatgtccc
     1621 ttccaacatt caaacgtggg cttggggagt tggtaaaatc acaaatatta gcaatgaata
     1681 agcgaaaggg attttattat ataaatccta attttgtttt taatggcgat agagttgcat
     1741 tcacaacagt tcttgaaatg aataaatcga gccataaaga caaatgacaa tgtattaagc
     1801 ccgtcaaagg aaaatttcaa atctattggc gggttttaat attcgtaacg gctagcgccg
     1861 aagattttag ctaaatatat ttgagccagg gtatttattt ttatgatgcc gttttattat
     1921 ttttatttcc tgttttaata gatccatttc attatatata tcagaaattc gctgtgttac
     1981 ctctttcatt tcagcacaaa gtgaaattat tttatgcatc acatctaggt actctttgtc
     2041 cttcggcctc ttcccgttat caaataattt cttctgcatc cgaccaagtt gtattaattc
     2101 atctagatat ctggaaggca cgcgctgttt tatttcatta tttaaacttg ccgtcctgat
     2161 atattctgaa attgtcagtc cttcgttctt cgctcgacgt ctgatttcgc ttgcaactct
     2221 gttatttact ctaagggtga taaccttagt tctctgccgt acttctgaac cactacgttt
     2281 atttttagcc atcgcaatga ctctccattt tgcacgtttg gggtgtcggg gcttgccctg
     2341 acaggaggta ttctcatgca agcgaatgta ttcgcggtat gacgaataca catcctgtcc
     2401 cgttttccgc tataactttt cggcgaaaat aaaataccgc gtcgtaacct agtatttctg
     2461 atac
//
LOCUS       DAZFVO020000309         2439 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFVO020000309 DAZFVO020000000
VERSION     DAZFVO020000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.311"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..2047
                     /locus_tag="ACHKNB_RS25375"
                     /old_locus_tag="pgaptmp_005075"
     CDS             <1..2047
                     /locus_tag="ACHKNB_RS25375"
                     /old_locus_tag="pgaptmp_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGU4198413.1"
                     /translation="KSRKSVKSVEPDIADRANSWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGAGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDDTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDTRPTLEDFKKTVGDYLAWEVSKLLQGRDASEKKQ"
     gene            2080..>2439
                     /locus_tag="ACHKNB_RS25380"
                     /old_locus_tag="pgaptmp_005076"
     CDS             2080..>2439
                     /locus_tag="ACHKNB_RS25380"
                     /old_locus_tag="pgaptmp_005076"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGU4198414.1"
                     /translation="MKSGGKWEEYTIERLFSIQTPKKKFNANKLTFNGNYPYVARGSN
                     NNGIKGYIDEDELYLNPGNTISFGQDTATMFYQENAYFTGDKIKIFSLRHGELNSRIA
                     VFLISAMKKSFSTFSWGS"
BASE COUNT          765 a          455 c          580 g          639 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacagctg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcgca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggcag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat gatacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaactgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccaccgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctggcc gattttgacg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag ataccagacc gacactggag gattttaaaa agacggttgg
     1981 cgactatctt gcgtgggagg tgtcaaaact tcttcagggg cgtgatgcgt cagaaaaaaa
     2041 gcagtaaact cccgtcttgc agagcttgag cgggagttta ttaaatctgg tggtaaatgg
     2101 gaagaataca caatcgaaag attgttcagt attcagacac ctaaaaagaa atttaatgcc
     2161 aataaattaa catttaatgg caactatcct tatgttgcca gaggaagtaa caataacggc
     2221 ataaaaggat atatagatga agatgaatta tatctcaacc cggggaatac aattagcttt
     2281 ggtcaggata ctgcgaccat gttctatcag gaaaacgcat atttcacagg tgataaaata
     2341 aagatttttt ctctcagaca tggagagtta aattcgcgaa ttgctgtttt tcttatatca
     2401 gcaatgaaaa aatctttttc aacgtttagt tgggggagt
//
LOCUS       DAZFVO020000310         2413 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFVO020000310 DAZFVO020000000
VERSION     DAZFVO020000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.312"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..530)
                     /locus_tag="ACHKNB_RS25385"
                     /old_locus_tag="pgaptmp_005077"
     CDS             complement(<1..530)
                     /locus_tag="ACHKNB_RS25385"
                     /old_locus_tag="pgaptmp_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198415.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELASNLTRSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGA"
     gene            complement(625..978)
                     /locus_tag="ACHKNB_RS25390"
                     /old_locus_tag="pgaptmp_005078"
     CDS             complement(625..978)
                     /locus_tag="ACHKNB_RS25390"
                     /old_locus_tag="pgaptmp_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198416.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACFKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(1114..2310)
                     /locus_tag="ACHKNB_RS25395"
                     /old_locus_tag="pgaptmp_005079"
     CDS             complement(1114..2310)
                     /locus_tag="ACHKNB_RS25395"
                     /old_locus_tag="pgaptmp_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4198417.1"
                     /translation="MKKASKLLLALMPALSLSLFSAGAYASGYSVTPESLNSNSMTQG
                     DFLKGIGVYPKEGGDPLIKLVDGKVTPLKGNTPTSLNIDAVSGKYLKYTLIDYGKAGL
                     TDTELVAPRGDIVSYYDYANETAYFVNTKTNQILFSADGEGVVSTVGADGKTYSRHIP
                     EISEYIYEIKMKAVQYGATIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAGGENAT
                     AVGYNATANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLERYRI
                     AEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIAGAM
                     AMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSGVAA
                     GMSVGW"
BASE COUNT          643 a          654 c          484 g          632 t
ORIGIN      
        1 gcgccaacaa gagaggctcg cccctgctgt ttcgtaaacg ccgcctgtgc atccgattta
       61 gcttttgcca gactgctgtc tgccagagag ttaaaactct gtaacgcatc cgccttctga
      121 ccagaaccga gagcagcaac atcctgtagc ccggcaacat acttatctgc ctgcgatacc
      181 tgcccccgtg tggttgtgtc aatctgcccg gttacctgat cactctggtt cgcattcatt
      241 accgcgttaa agcgaccact ggacgggtca acaccggacc gagtgagatt acttgccagc
      301 tccttccgcg cttcgccaaa ctgtttttga taccccagat ttgtggtgcc agcaatattg
      361 tcgtactgct gctcactgtt caggtcatcg accttttcca tgaagttatc ttcagccggg
      421 cggaggatat ttttgtaatc ctgccaccct ttccaggcca cttcttcctg tgctatttgc
      481 gctgctgttg gttttacttt ggtatcacca ccacctttac ttccacccat aatggccccc
      541 tggataataa aaaaaccctg ccggagcagg gtcagaatgt gaattgcaat gttggtttaa
      601 acacgatgat gaaaactgtg gcagtcagac cgctatcctg aacaccatga agccatcctc
      661 atcatccggc attcgctcaa agcccagacg ttttcccagc cggataaatc cccgccttgc
      721 cgtatgaaat tcagcccaac gtccgccagc cagacgggtt aatgtcttca cctccggcag
      781 ataacgctca acactgttac tccccgtaca cacgcccagc aacaccagaa cataagggat
      841 accatcagca ctgagcacag aacgcagcac cagaaagcca tcaggtgcct caaaacaaaa
      901 cgcctgcttt ttaaagcagg cgtctttaac ttcattcata aattcagggt tgcgggaatt
      961 tctcaccaca cgctgcatat accggagaat tttttcactc acactctcac cacaaaaaag
     1021 aacaatgtga ctgtgctgtg tcttcagcga cagaaggatg acggagaacg cttccgataa
     1081 ccaacacggc tggcgttctc cgcaaaaaat aaattaccag ccgacagaca tacccgcagc
     1141 aacacctgaa ccgttctggg tatcccatga agcattcaaa cgtacagcag aattttcagt
     1201 ggtgcggaag tgcatacctg ctgcgatagc ctgctcatca cggtagttac ttgcagccat
     1261 accaaaggag tacgttttac cctcaacaac aggaatggca gtcatcgcca ttgctcctgc
     1321 aatacctgca tctgcacgtt tacggttttt ctccacctca tcacgtaact gacggaattg
     1381 ctgattagta ctacgctcca gggtactgac ccggttatct gtgtaggtat tagcagactt
     1441 cagtgtctgt gcatcaccct gaatacgggc attctcttct gcaatacgat agcgctctaa
     1501 ctgaccatag gttgcggcat ccgcagcctg aacaccatca cgaagcccac caattacacg
     1561 attccccaca tttacttcat cggggcgagt ggtctgagag ttagccccta cagcaacact
     1621 gttcgtatgg tttgcagtgg cgttgtaacc gacagcagtg gcgttttctc cgcctgctgt
     1681 tgccccgcca ccaacctgag ttttaccggt gcccagagaa gacagacggg cgtgaatatc
     1741 tgttacatct gcagcagaaa gaccagtgtc aatagtggct ccatattgca cagccttcat
     1801 tttaatttca taaatatatt cactaatttc aggaatatgt cttgaatatg tttttccatc
     1861 agcgcctact gtcgagacaa ctccttcgcc atctgcacta aacagaattt gattggtttt
     1921 tgtgtttaca aaataagctg tctcattcgc gtaatcataa tatgagacga tgtctcccct
     1981 tggagcaact aactcggtat ctgtcaggcc agctttacca taatcaatta aggtatattt
     2041 caaatattta ccggaaacag cgtctatatt taatgaggtt ggtgtattcc ctttaagagg
     2101 agtaacctta ccatcaacaa gcttaattaa aggatcccct ccctcttttg gatatacacc
     2161 gatacctttc aaaaagtcac cctgagtcat tgaattacta ttcagacttt caggtgtaac
     2221 ggaataacca ctggcatacg ccccggcaga aaataatgac aggcttaatg ccggcattaa
     2281 agccaacaac agcttgcttg cctttttcat gatgtgcacc ctatgttaaa tcatgttata
     2341 aatatgttga cattcggaac gcaaatgtct tcagggatac tactcataat aacaaggcaa
     2401 ataaaatatt aaa
//
LOCUS       DAZFVO020000311         2400 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFVO020000311 DAZFVO020000000
VERSION     DAZFVO020000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.313"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            72..452
                     /locus_tag="ACHKNB_RS25400"
                     /old_locus_tag="pgaptmp_005080"
     CDS             72..452
                     /locus_tag="ACHKNB_RS25400"
                     /old_locus_tag="pgaptmp_005080"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4198418.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            449..796
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS25405"
                     /old_locus_tag="pgaptmp_005081"
     CDS             449..796
                     /gene="tnpB"
                     /locus_tag="ACHKNB_RS25405"
                     /old_locus_tag="pgaptmp_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGU4198419.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLPSLTML"
     gene            846..2384
                     /locus_tag="ACHKNB_RS25410"
                     /old_locus_tag="pgaptmp_005082"
     CDS             846..2384
                     /locus_tag="ACHKNB_RS25410"
                     /old_locus_tag="pgaptmp_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HGU4198420.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          555 a          665 c          696 g          484 t
ORIGIN      
        1 tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca
       61 aagtatcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc cctaattatc
      121 ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa
      181 aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc
      241 gcgagggaaa actgctatta ccttcttcag agagccccca gctacttcct gtgactctcg
      301 atgccgctgc cgaacagcca gaatcgctcg cagaggcccc ggaaaccctc agtatcagct
      361 gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc
      421 tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga ccaaaatttg
      481 gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg cgaaagtaca
      541 aacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg gccgcagcgg
      601 cagtcaggtt aaactgctgt ggtccaccgg tgacggactg tgcctcctga ccaaacggct
      661 ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc ttacgcaggc
      721 gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc tgctgccctc
      781 cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg gtaaaatacg
      841 ggcttatgaa cgacatctct tctgacgaca tcttcctgct gaaacagcgc ctggccgaac
      901 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
      961 atctgcaggc gcagttggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     1021 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa gagagcgata
     1081 cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     1141 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt
     1201 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc gaacagctgg
     1261 agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat gcctgtactc
     1321 agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg ggtatcgccg
     1381 gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc ccgctgtatc
     1441 gccagtcaga aatatacggc cggcaaggtg tggagctgag gcgttcactg ctgtcgggct
     1501 gggtggatgc atgctgccgg ctgctgtctc cgctggaaga ggcgcttcat ggctatgtca
     1561 tgactgacgg caaactccat gccgatgata ccccggtcca ggtactgctg ccgggtaata
     1621 agaagacgaa gaccgggcgg ttgtgggcgt atgttcgtga tgaccgcaat gccgggtcag
     1681 cgttggcacc tgcagtgtgg ttcgcttaca gcccggacag aaaaggcatc catccgcaga
     1741 ctcatcttgc ctgcttcagc ggtgtgctgc aagcggatgc gtacgccggg ttcaacgagc
     1801 tttatcgcaa tggtgggata acggaagctg cctgctgggc tcatgcccgc cgaaagatcc
     1861 acgatgtgca cgtccgcatc ccgtcagcac tgacggaaga agccctggag cagatcggtc
     1921 agttgtacgc catagaggcg gatataaggg gaatgccggc agagcagcgg cttgctgaac
     1981 gtcagcgaaa aacgaaaccg ttgttgaaat ccctggaaag ctggttgcgt gaaaagatga
     2041 agaccctgtc gcgacactca gagttggcga aggcgttcgc gtacgcactt aaccagtggc
     2101 cggcactgac gtactatgcg aacgatggct gggtggaaat cgacaacaac atcgctgaaa
     2161 atgccctgcg ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc tctgaccatg
     2221 gtggtgagcg gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg
     2281 atccagaaag ctaccttcgc catgtgcttg gcgtcatagc agactggccg gtcaaccggg
     2341 tcagcgaact gcttccgtgg cgcatagcac tgccagctga ataacacatc cccgtcaata
//
LOCUS       DAZFVO020000312         2393 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFVO020000312 DAZFVO020000000
VERSION     DAZFVO020000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.314"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..554)
                     /locus_tag="ACHKNB_RS25415"
                     /old_locus_tag="pgaptmp_005083"
     CDS             complement(<1..554)
                     /locus_tag="ACHKNB_RS25415"
                     /old_locus_tag="pgaptmp_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HGU4198421.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILY"
     gene            complement(563..1330)
                     /gene="sctT"
                     /locus_tag="ACHKNB_RS25420"
                     /old_locus_tag="pgaptmp_005084"
     CDS             complement(563..1330)
                     /gene="sctT"
                     /locus_tag="ACHKNB_RS25420"
                     /old_locus_tag="pgaptmp_005084"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HGU4198422.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1332..1592)
                     /locus_tag="ACHKNB_RS25425"
                     /old_locus_tag="pgaptmp_005085"
     CDS             complement(1332..1592)
                     /locus_tag="ACHKNB_RS25425"
                     /old_locus_tag="pgaptmp_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4198423.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1602..2267)
                     /locus_tag="ACHKNB_RS25430"
                     /old_locus_tag="pgaptmp_005086"
     CDS             complement(1602..2267)
                     /locus_tag="ACHKNB_RS25430"
                     /old_locus_tag="pgaptmp_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HGU4198424.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2257..>2393)
                     /locus_tag="ACHKNB_RS25435"
                     /old_locus_tag="pgaptmp_005087"
     CDS             complement(2257..>2393)
                     /locus_tag="ACHKNB_RS25435"
                     /old_locus_tag="pgaptmp_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HGU4198425.1"
                     /translation="LPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK
                     "
BASE COUNT          836 a          493 c          382 g          682 t
ORIGIN      
        1 caatacagta tgagaaggaa tagcatctct ccccaatctg ataataaaga tatgatatct
       61 ccatcaagag ttttaaaaag cagtgattta tgattactcc aaaatacttt gatcgccaat
      121 gcaaaaaaaa caatataaag aattgcttta acaaattctt ttacggtttt taagccaaat
      181 atacgcttta agccatttac tggattcaat gaatcaaact taaatttaac agcttcagtt
      241 gctaattgca ttttactttg aaaccaggag acaagtattg ttgtgaacat gcaaaccagc
      301 aaaaaaggaa tgttcgtttt caaccaacca acccccatag ctttaaaata gtcccaaata
      361 tctggtttag cgttatgatc aaggatgtat tcaagaatcg acataatgtg atgcacatca
      421 aaaacatatc cgagatataa agtacccaca agcattatta cagagatggt taagtctcta
      481 ctttttagaa tttgcccttt tttagaagca tcctggagtt tcttttgtgt tggcttttca
      541 gttttattgg ccatgaaaca ctctatttct caatgtatag ttgcaattgt tctgggaaaa
      601 aggataacgg cattactcgt tcagccagta ttggggcaaa ataaataatt aatattaaaa
      661 atgccagcgc acttttgaca gtcagtgaaa tggcaaaagc atttagttgt gaagcataac
      721 atgccagcaa tcccaggaca gcctcaccgc ccagcataac ggcaatcacg gggcttgcgt
      781 aaacgatagt atgagtcatt atgttattaa tatatgagaa taaaggctct aatttaggaa
      841 aattaaaatt gcctgaaggc cataaattat aactttgcca aagagtttcc agaataaagt
      901 tcattccacc tttagtaaga taaaccgcag cagagaaaag gttaaataat cttgctaatt
      961 ccgaggtatc gacacctgta gccggatcca aagtactact taatgttgca ccgcgttggt
     1021 tatcgataaa gctacctata gcaaggaaca tccaaaacgg ggatgctaat aagcatgcaa
     1081 taaacagccc tatgataatt tctctggcag cgaacaaagc aatatcgatt tcatttaagt
     1141 tcaataattc atagtggtaa tgctgaacta atgcacatga taccactatt attattgggt
     1201 gacgaactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag aaagttggtg
     1261 ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga tagagtatcg
     1321 cctcccccat gctatcctct cgcgaatgct aaattcagca tttcgatacc aaatgaatat
     1381 aatttttcac cataccaccc gctcattaaa aaaaagcaaa tacttacgca caataatttc
     1441 acgccaaatg gcaacgtctg ttcttgcaac tgcgttaccg tttgaaaaag ccctaccaat
     1501 aaaccaacaa atgtcgcaac tgcaataggt cctgcagaca taactaaaat cagatacagc
     1561 gccctattcc ccgcaaaaac aatatcgtcc atattttttc ctcagggatt aatggatagg
     1621 tcaaaatatt gtaaaatgag cccttttgat aacattgtcc aaccgtccat cgcgacaaac
     1681 aaaattaatt ttatcggtgt agatatggtt accggactca tcatcatcat acctaacgtt
     1741 aacagaacgc tggatataac cagatccact acgacaaagg gtaaatagat ataaaaacct
     1801 ataataaatg cactttttat ctcactcaat gcatatgcgg gcagtaacga gaaaattgaa
     1861 atattatcat catcgattat ctcttcattg tcttcagaac tattcacctt ctgaatcttt
     1921 tcaaaaaagc taaccaactc aggttcagaa tattttatca aatatgattt ataaccactc
     1981 atgcctgtct caacaaaatt aacaaccgat gctacattat tgaatgaaag attctcattc
     2041 tgactgttat tatatatttc cttcccaacc ggcatcatta cgaacattga caatagcaaa
     2101 gcaaccccat taagtgtcat atttgatggc acttgctgaa gtcccagcgc gtttcggaca
     2161 ataacaaaaa caatcgagaa tttaataaaa catgtcccgg atgctattat aaacggcaat
     2221 agtgtaaata atgataatat cgctatcagc gaaatgctat ttgacattat tattccctga
     2281 taaccaagaa tgaatttcta cgcccaattt atcatccact tcaacaagtt cgccatagcc
     2341 agttagcgca ccatttacac gtatctctat atttttctca gattcaggaa gca
//
LOCUS       DAZFVO020000313         2382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFVO020000313 DAZFVO020000000
VERSION     DAZFVO020000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.315"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            281..475
                     /locus_tag="ACHKNB_RS25440"
                     /old_locus_tag="pgaptmp_005088"
     CDS             281..475
                     /locus_tag="ACHKNB_RS25440"
                     /old_locus_tag="pgaptmp_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198426.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGVWL"
     gene            472..2361
                     /locus_tag="ACHKNB_RS25445"
                     /old_locus_tag="pgaptmp_005089"
     CDS             472..2361
                     /locus_tag="ACHKNB_RS25445"
                     /old_locus_tag="pgaptmp_005089"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HGU4198427.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVIPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTGSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYSTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT          695 a          560 c          601 g          526 t
ORIGIN      
        1 tttgttcatc accggaacgt tcccgaaaac atttacaaaa aacaggcgca aaaaaagcgc
       61 ccccgattgc tgttaccgga ggcgctttta cacgacaaag gagtttttat cgccaggatg
      121 acgagtctta atactgcttc aatggcaaaa atgcgtcaat aactttgcct ttctgagaat
      181 aatcagaaaa atcataatct gattttcagg tagaaaatga tttatctatt acttttatcg
      241 atcaataatg atgcccgtta atcaaaacgg aggcggattt atgccagaga acaacaccag
      301 aaagccggat aaaagtgcca cggtacacat agacgccgga actatggaga agatcgaacg
      361 ctatcagcag ttcatcaaag ataatcaccc gggtatgcca gtacccacga aaggacaaat
      421 cacacgcagc gcggttgaat actggtacag ggcaacgtta ggggtctggc tatgaaaaca
      481 tggttttcca ttaaggctat ggcagatgtt gtctatgtgc gcatttatga cgagatcggc
      541 gggtacggtg taaaagcatc ggcacttact gacgagatca acgcgtgtgg taatgcgtct
      601 gaaatccatc ttcgcatcca ttcacctggt ggcgacatct ttgaagggct ggctatctat
      661 aacgccctga aaaatcatcc ggcaaagaaa attgtacaca ttgaaggcat ggcggcttct
      721 atggcctcgt ttattgccat gtgtggcgat cacatcgtta tacctgaaaa cgcgatgatg
      781 atgatacatg ccccccgtgg tgttactgcc ggagtgtcgg gcgacgttcg ccgctttgct
      841 gacctgatgg acaagctggg cgacacgatg gcggaaacct acgccggaag aacgggcagg
      901 agcaaacagg aaatcaccgc catgatggag gcggaaacct ggatggatgg caatgagtgt
      961 aaggctaacg gcttcgcaga tgaggttata cccgcgatta cagcaatggc ccgaattgaa
     1021 tcaaaacgaa tcggagattt ttcaaatatg ccggaaaaaa ttaaaagcat gatcagccag
     1081 aaaactggca gtggcgaaca ggaacgactt aacggcatcc gtgaattgtt tggcacgttc
     1141 aacggaagat ataacgacct ggctataagt tgtcttgctg attcagaatg tagcgttgag
     1201 aatgcacgcg aacgcctttt actcgctatg ggtaaagaat caacgccaac aaacaaaacc
     1261 acccccgcaa atctttacta cgcgtacacg gataacggca acataaccgg cgatgcaatg
     1321 cgccaggggc ttaatgcgcg tcttggtcac gaacgggccg aacgcggtaa tccttacgcc
     1381 atgatgagcc ttttcgatat ggcacaggca tcattaaccc atcgtggtat aagcacgggc
     1441 agctacagca cacgctcgca gatagtaaac gcggcattca cccacagcag cagcgatttt
     1501 accgatatcc ttgctggtgg cgctgaaaaa tcagtgcttg caggctggga gcacagcggc
     1561 gaaacattcc gccagtggac gaaaaaaggt tccctttcaa acttccggga agcccgccgc
     1621 gttggtatga atggcttctc aacgttaaac aaagtgccgg aaggggcaga atataaatac
     1681 atcaccacca gcgatcgcgg tgaacccatc gcgctggcta cttacgggaa tattttcagc
     1741 attacccgcc aggcgataat caatgatgac cttgatcagt tatcaacggt gccaatggct
     1801 atgggccgtg ctgcatcaag aacggtggga aatctggtta atctggtgct tacaggcaac
     1861 gtaaaacttt ctgacggaat agcgctgttt gacaaaaaac acagcaacct gattgaagca
     1921 ggactgacaa caccgggact tagtgcagca cgtcacctga tgcgcacaca gaaggacaaa
     1981 aatggcgaag tgctgaatat tgcgcctaaa ttccttttag ttccggcagc actggaagat
     2041 cgcgcgttgc agatgattaa ctcaaccgca cctttcgggg ctgataaaaa cagcgggatc
     2101 tttaacccgt atcacaagct acttgatatc atcgtcgatc cccgccttga tgatatcagc
     2161 gaaaaacaat ggtacatgct ttccgcacag ggaacggaca caatcgaggt ggcttatctt
     2221 gatggcaatg acgagcctta ccttgaacag caggaaggtt ttatcgttga cggcgtggcc
     2281 tggaaagtcc gtattgatgc aggtgtggca gctctggatt atcgcggtat ggtcaaatca
     2341 ggcgggacag attcactatg acaacaaggc ggcaccagcc gc
//
LOCUS       DAZFVO020000314         2357 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFVO020000314 DAZFVO020000000
VERSION     DAZFVO020000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.316"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(120..1781)
                     /locus_tag="ACHKNB_RS25450"
                     /old_locus_tag="pgaptmp_005090"
     CDS             complement(120..1781)
                     /locus_tag="ACHKNB_RS25450"
                     /old_locus_tag="pgaptmp_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4198428.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGTHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(1778..2341)
                     /locus_tag="ACHKNB_RS25455"
                     /old_locus_tag="pgaptmp_005091"
     CDS             complement(1778..2341)
                     /locus_tag="ACHKNB_RS25455"
                     /old_locus_tag="pgaptmp_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4198429.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMS
                     PGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDAAWRLAQFTHHKKGDTGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          488 a          755 c          584 g          530 t
ORIGIN      
        1 tcatcctcac tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca
       61 gcctcctgaa aataaaaaaa ccgccggagc ggttcgtgat ggttacagtg tgaacagggt
      121 tatatgaaaa aaaccgcata ttctttcttt ttcggttccg ggttaaggga catcagggag
      181 accgcattga agagcgccat cagcgggtca atttttcccc gcccactggc ctgtttggtg
      241 ataagaatgg cgttaccttt aggctccacc cgggcattgc cgacacacca ggccatcagg
      301 ggctggtcac catgcaccag caccccttca gccagtttgc gctcggtggt tttaatggcc
      361 ccgcccagtt tccagccctg gcttatcccc accacaattc cgtcggggat cccggcttcc
      421 gccagtgaat ccagaatctg ccccacccct gacgggtcaa taccgatatg ctccagtaac
      481 tcagcctcat gaatgcgacg cacatattcc gccacttccg ccgtgtcatc cccgacacgc
      541 cggacaatgg tcatatctcc acaggcaaca agatcctgaa accgggacgc ctcgctcttc
      601 cgtcggacca ccgcggtttc atgcgcccag gcatggcccc agcccagcca ttcgcgggtc
      661 tcccggtcac gcccaatcac atacatcccc agcagatcat ccagccctcc gccgtcaatc
      721 cccaccgtca ccacatcagc acgacgcagg atatcgtcca ggctgataca acggccctgc
      781 tcttcccaga aatcagcccc cgcccagcgg tcagagcgca gggcaagacc aatttccaca
      841 ttggcgtgtt ttgacatgaa cccccggaat gtctcttcac cggcttcccg ggctttacgg
      901 tactcccggt acagaaaggc ctcatccact gaatagccga gattcggatt gaccatggcg
      961 aggttttcca tcagcaggtg agtcccgctt tccaccattt caggagggtg ttcaaatatc
     1021 accggcagaa agtgcggatc atgaattttg ccgtcgcgca catcccgggc gtactgcagt
     1081 ttctgtctga acaccccggc gggcggttca ttcgactggg tggtcgtata caccacaaac
     1141 ccttccgggc gggaggcaag gccgcctatg gcttcacgta acatgtcctc cgccttgcac
     1201 tgcttgccaa acagccacaa ctcatcaatc agcgtaccca cggacttgat accggacacc
     1261 gtattcggat cggctgccac caccttcagg gtggtgtctg tcaccctgtg ggtgatggtc
     1321 cggatatggg tctgtacctg gcagaggtca tccagatcat cgtcacgtcg taccatatcc
     1381 cgggcagggt tgaaggcgtt ggccgccacc tccacagtcg gggccagaat cgtgtaaccc
     1441 gccgcctgcc gccagttcag taacagcgcc gtcatcatga tccccgcggc cagcgtggac
     1501 ttcgagtttt tcttggggat aaggataaaa acttccttga tatggcgtac accggtctgc
     1561 gcatcgtagg agccaaacag ggccgccacc aggtcaaaca cccacggtgc acaggactcc
     1621 ccgaacgtag ggctaccagg tgcatccaca atccgcagtt gtttaaaaat cgccagggca
     1681 tgtgcggcct cgtccggata aatcggatcc ggaataatcg acagcccctt tttcaggcgc
     1741 tctgcccagt ccgggcaggc tgtgctccat acaggtatca tccgttgccc tcattatcgt
     1801 tattcaccac cagtctgggt ggcggtggca ccgcaaaacg gttagccgct tttttcgcgg
     1861 catcaccttt tgccgatttt ttcccggtat cccctttttt atggtgcgtg aactgcgcca
     1921 gacgccaggc cgcatccagt gccagtttcg gatcaatgca gaggttttcc accaggatcc
     1981 gccccatggc tttcaccgga tcgggaagac catcctccat atattcaata ccaggagaca
     2041 tcaccgcgga cggtggcatc tccggattgt tttcgtccgg ctgtggtatt gcagccgcct
     2101 cacggcgacg gggtttatcc tcctgctctg attttttctg ccggtaaaca ggaacctcat
     2161 ccacctccac cgtctcgcat tgtttacggg ctataaacgc aagcacctcc ggatcttttg
     2221 ccagctgcga gcctttaacc ctggcggtct tcgccgaata accggcggca atggctgacg
     2281 ctgttttgtt tttcccggac atgagcgcca gcgcaaattt tcgtttttgc gttgtcagca
     2341 cagcctcctc ccgggtc
//
LOCUS       DAZFVO020000315         2339 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFVO020000315 DAZFVO020000000
VERSION     DAZFVO020000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.317"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..185
                     /locus_tag="ACHKNB_RS25460"
                     /old_locus_tag="pgaptmp_005092"
     CDS             <1..185
                     /locus_tag="ACHKNB_RS25460"
                     /old_locus_tag="pgaptmp_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198430.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDAVVRETVLRDFNTPPFKTE
                     PDDWIQPGLNYLKGRI"
     gene            complement(197..511)
                     /locus_tag="ACHKNB_RS25465"
                     /old_locus_tag="pgaptmp_005093"
     CDS             complement(197..511)
                     /locus_tag="ACHKNB_RS25465"
                     /old_locus_tag="pgaptmp_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198431.1"
                     /translation="MHNHQHNYDLCLQAINERVKSECLLLLPQEHKAVKAIQAEPYGA
                     LTTATLSIISGALTPTLLLRLKDNIDDWLNEELSYLDCEWDNHYAKSQKDRLFRQLSG
                     NR"
     gene            640..1173
                     /locus_tag="ACHKNB_RS25470"
                     /old_locus_tag="pgaptmp_005094"
     CDS             640..1173
                     /locus_tag="ACHKNB_RS25470"
                     /old_locus_tag="pgaptmp_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HGU4198432.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVIPGMKLSKEKCDQVNAIERDKALTWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1394..1507
                     /locus_tag="ACHKNB_RS25475"
                     /old_locus_tag="pgaptmp_005095"
     CDS             1394..1507
                     /locus_tag="ACHKNB_RS25475"
                     /old_locus_tag="pgaptmp_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198433.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            1509..1976
                     /locus_tag="ACHKNB_RS25480"
                     /old_locus_tag="pgaptmp_005096"
     CDS             1509..1976
                     /locus_tag="ACHKNB_RS25480"
                     /old_locus_tag="pgaptmp_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HGU4198434.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENEILRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIK"
     gene            2000..2224
                     /locus_tag="ACHKNB_RS25485"
                     /old_locus_tag="pgaptmp_005097"
     CDS             2000..2224
                     /locus_tag="ACHKNB_RS25485"
                     /old_locus_tag="pgaptmp_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198435.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          587 a          518 c          650 g          584 t
ORIGIN      
        1 agcgtattgg tcgttgcatg ggatatcttc aggtagctgc tgagattgaa gcgctggcca
       61 gtggtgctga caaggatgca gttgtgcggg agaccgttct tcgtgatttc aatactccac
      121 cctttaagac tgagccagat gattggatcc agccggggct gaattatctc aaagggcgga
      181 tataagttca ggggcgttat ctgttacctg ataattgtct gaacaggcga tctttttgtg
      241 atttggcgta atgattatcc cattcgcaat caaggtaact taattcttca tttaaccagt
      301 catctatatt gtctttcagg cgaaggagca aggttggcgt taatgctccg gatataatac
      361 tgagagttgc agtagtgaga gctccatacg gttcagcctg aatggccttt actgctttgt
      421 gttcttgcgg gaggagtaag aggcattccg attttacccg ctcatttatg gcttgcaggc
      481 ataaatcata attatgttga tggttatgca tggttaatcc tcgactggaa ttgtcagata
      541 tattttcagc cagcagataa aacgccagtg cccaccactg gcgggctgaa gacttaacat
      601 atccagggat tcggaaccga taaatcctga taaatatcca tgaacgcaaa aatcagatac
      661 ggcctgtcgg ctgccgttct ggcgctgatt gccgcagggg cgcctgcgcc tgatattctc
      721 gaccagtttc ttgacgaaaa agaaggcaac cacaccacag cataccgtga tggtgcgggt
      781 atctggacca tctgccgagg tgccacccag gtggatggta agcctgtgat tcctggcatg
      841 aagctgtcga aggaaaaatg cgaccaggtt aacgccattg agcgtgataa agcgctgacg
      901 tgggtggaga aaaacatcag agtgccgctg accgaacccc agaaagcggg gatcgcgtca
      961 ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt
     1021 aatgcaggtg atcgcagggg agcatgcgaa gcgattcgct ggtggattaa ggacggtggc
     1081 agagactgcc gtattcgctc aaataactgt tacggtcagg tatcccgtcg tgaccaggag
     1141 agcgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt
     1201 tgaccaacgc ggacggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa
     1261 gtaaaaaccc cgtttgttgg cagcaagcgg ggttttgtgt ttctgacctt ggataaggca
     1321 agggagaaat tatggtgagg gaggtgcttt ccctgtgagg aagtataaaa gattctttct
     1381 gaggttgtcc attatgaaag gcattgaagt ggagacgcca gccagtctgg atttaacaag
     1441 agcggcagct tttgccattc gtattatagc cattgctgtt ctgatctggg caatccgttg
     1501 gtggtgaaat gaaccgcgtt ctgtgtgtgg tcatcattgc tctgctggtg gcctgtggtg
     1561 cgcttagtct ggggctgaat cattaccgcg ataacgccat cacttacaaa gcgcagcgcg
     1621 ataaaaaagc cagagagctg gagctggcaa acgcaaccat tactgatatg caggtgcgcc
     1681 agcgtgatgt tgctgcgctc gatgcaaaat actcgaggga attagccgat gcgagagctg
     1741 aaaatgaaat tcttcgcgct gacgttgccg ctggtcgtaa gcgcctgcgt atcaacgcca
     1801 cctgtccagg tcccgtgcgt gaagccaccg gcaccgcccg cgtggataat gcaaccggcc
     1861 cccaactggc agacaccgtt acacgggatt atttcaccct cagagagcgg ctgatgacga
     1921 tgcagaagca actggaaggg gcgcaggaat atatccgtac tcagtgcatt aagtagtctt
     1981 tttattatcc ggaggatata tgaagaaatt actggtaacc gtaaagcctt ttcagggaac
     2041 aattccgttc cgtgttttgc agcctggtcg tgttcttgtt gaaggtacgt tcagtggtaa
     2101 atgtacgcaa tcatactccc gggtctttcg ggtggatgcc tcgaatgaag agctaaccgt
     2161 ggagtgtgct gtgaatgccg ctaaatgccg catggtatcg gctgcattac agccagtgtg
     2221 ttgagcgacc ttattatcca tggcgcggta ttgtcgccgt attcccgtat taacagagac
     2281 cgcagcccga cagggagact cctctgcgcg agtgtgcggg gataatcaaa aacgataca
//
LOCUS       DAZFVO020000316         2306 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFVO020000316 DAZFVO020000000
VERSION     DAZFVO020000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.318"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..240
                     /locus_tag="ACHKNB_RS25490"
                     /old_locus_tag="pgaptmp_005098"
     CDS             <1..240
                     /locus_tag="ACHKNB_RS25490"
                     /old_locus_tag="pgaptmp_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198436.1"
                     /translation="VKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQL
                     TIRYSNQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            255..533
                     /locus_tag="ACHKNB_RS25495"
                     /old_locus_tag="pgaptmp_005099"
     CDS             255..533
                     /locus_tag="ACHKNB_RS25495"
                     /old_locus_tag="pgaptmp_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198437.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            539..1156
                     /locus_tag="ACHKNB_RS25500"
                     /old_locus_tag="pgaptmp_005100"
     CDS             539..1156
                     /locus_tag="ACHKNB_RS25500"
                     /old_locus_tag="pgaptmp_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198438.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            1247..1981
                     /locus_tag="ACHKNB_RS25505"
                     /old_locus_tag="pgaptmp_005101"
     CDS             1247..1981
                     /locus_tag="ACHKNB_RS25505"
                     /old_locus_tag="pgaptmp_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HGU4198439.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            2213..>2306
                     /locus_tag="ACHKNB_RS25510"
                     /old_locus_tag="pgaptmp_005102"
     CDS             2213..>2306
                     /locus_tag="ACHKNB_RS25510"
                     /old_locus_tag="pgaptmp_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4198440.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRL"
BASE COUNT          642 a          441 c          577 g          646 t
ORIGIN      
        1 gtaaaagccg gggtaagtat cacttcgcca gttatccgga gtgccgttat tcagaacgga
       61 aactttcagg ttgattctca gggtaacctg aatattggag gccttttcag tgttacgtca
      121 caagggcaac tgacaattcg ttactctaat cagaatgtag gactggtgat ccgcaatgat
      181 aaaattgagg tttatgatca gaatggacga ctggctgttc gcataggcag attacgctga
      241 tcaggaggtg agtattggaa tacggttttg ccatttataa cagaaataac gttaatgtta
      301 cgggcgtgct gactccggta tttttcctgg acagatttac agcggagtct ggctcaaaga
      361 cgtacacgaa taaacccgac gggaaatcat tgcaggctgt atgttgttta tttccctgga
      421 ataacgtatt tgcggatcgg aaagtaccga agataaccat taatggcaat acggtgacgt
      481 ggtcgaatct tgagcagggt atgggatctt atatttatac attctgggga taagtgtcat
      541 gtatggtttg agcattatga agccggatgg cagcgtatgg ataagtccag gttttacgcc
      601 gcagtgtctg atcaacaaag gcaccatacc ggcgactgaa aagtcttttt ttaaaacatc
      661 aatcccgtca ggcaaaagtt gttttttctt tatcagaaca gagaagaagg ccgatgtcat
      721 gtacacgcat gaacagattg atggatatca tgcactaagg cttcatgtaa ttgtcagggg
      781 aacgaaccct ggtgttacga cggtttatgc tttcgcgaat atggttactc caccttctga
      841 gtatggtatc gccatgtata acccggacgg tgagatgatt tatcatggcg aaatgatgct
      901 gcttgacgcg aagttaatac ctgttgatat caaatttgaa aaggaccttg gatatccatg
      961 cgcaatcatg cctgcactgg tcgggtatta taactggaaa agaactcctt atgatcgacc
     1021 gatttatacc acatccactg gtgctacagg aaataaaata tattcctgtg agcattattc
     1081 cggtggtgca acatgggata ttcgaaagcc gtatatagat aaggtcctgg ttattaatac
     1141 atcagtatat gattagttga agcgagtctt taatattcat ttaaaatgtc taaaaagatg
     1201 tattattaaa aagtttagcg tgttatctga atacaggata tcttaaatga agagtatagc
     1261 aacactggtt gtgtgtgcaa tctccgggat tgcctgtgta aatttatctg cacatgcagc
     1321 agaaggaaac catacaattt ctctggggta tgcgcacttt cagtttccgg gactgaagga
     1381 ttttgtaaag gatgcgactg ctcataacag ggagactttc agtcatttcg tcaacagaaa
     1441 ctacttttct tcattgggcg aatatacaga tggtcgggtc agtggatatg aaggcaagga
     1501 taaaaatcca cagggcatta atatcaggta tcgctacgag ataacggatg attttggcgt
     1561 tatcacctct tttacatgga cgcgttctct cactaactca cagacattta ttgatgtgca
     1621 gtcagccgat cataccagga agattaagaa tccggcagct tctgccagaa cggatatcag
     1681 ggcgaattac tggagtctgt tagcggggcc ttcatggcgg gttaatcagt acatgagttt
     1741 atatgcgatg gcagggatgg gcgttgctaa agttagcgct gacctgaaaa ttaaggacaa
     1801 tattaacagt agtggcggat tttctgaaag caacagcacg aaaaaaacct cccttgcgtg
     1861 ggctgcaggt gcacagttta acctgaatga gagtgttaca ctggatgtgg cttacgaagg
     1921 ttccggctct ggcgactggc gcacgagtgg cgttactgct ggcattggcc tgaaattctg
     1981 acctgtatcc ggtaaccgtt tactacccgc tgtgatggcg ggttttttat tgcccgtaca
     2041 gggcaaaaac cgtaaattat gcgtgggtgc ctttcggctg atggctggag ggagaacctg
     2101 aaggcctgat gtggaaaggc cccgagtcaa cttaacgtta acccgaggcc ctaatcatca
     2161 taccttaagc aagtagaagg ttagcgcctc tctgtaaaag gagtcaagcg ctatgtcgca
     2221 aaaatcgctt atcaccgtca caatttgtat gacggttatc ttcactatct ggatgttgca
     2281 cggttcgctg tgtgagttcc ggctga
//
LOCUS       DAZFVO020000317         2280 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFVO020000317 DAZFVO020000000
VERSION     DAZFVO020000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.319"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..202
                     /locus_tag="ACHKNB_RS25515"
                     /old_locus_tag="pgaptmp_005103"
     CDS             <1..202
                     /locus_tag="ACHKNB_RS25515"
                     /old_locus_tag="pgaptmp_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198441.1"
                     /translation="AWGISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAAL
                     TEKHINAAFKEVYTNPELLSQV"
     gene            205..426
                     /locus_tag="ACHKNB_RS25520"
                     /old_locus_tag="pgaptmp_005104"
     CDS             205..426
                     /locus_tag="ACHKNB_RS25520"
                     /old_locus_tag="pgaptmp_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198442.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKAVSMSDSEECGDLLFEFNDRTR
                     MAISAGLDREGAKEAYSNICRFIGARRVS"
     gene            439..858
                     /locus_tag="ACHKNB_RS25525"
                     /old_locus_tag="pgaptmp_005105"
     CDS             439..858
                     /locus_tag="ACHKNB_RS25525"
                     /old_locus_tag="pgaptmp_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198443.1"
                     /translation="MKMQIQQEINVLHSFREFNNNDCEVRDYIYELMNGDGVCFLFID
                     LYQWYADQQCESVRQRFDYECFDVDVWEGLRFGFLIPGDYRREWRHSYSILRAIMRGE
                     TRKMPGMGKVNRGLLLEIAAAHMNPLLIQPEFTYEFV"
     gene            845..1075
                     /locus_tag="ACHKNB_RS25530"
                     /old_locus_tag="pgaptmp_005106"
     CDS             845..1075
                     /locus_tag="ACHKNB_RS25530"
                     /old_locus_tag="pgaptmp_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198444.1"
                     /translation="MNLFDSLNNARRLTELAGAVLERSKRYPQRFALKTTPPVGEVQG
                     SGEEIEITVKTNGLRRRVKATRISGCTVYWEV"
     gene            1150..1422
                     /locus_tag="ACHKNB_RS25535"
                     /old_locus_tag="pgaptmp_005107"
     CDS             1150..1422
                     /locus_tag="ACHKNB_RS25535"
                     /old_locus_tag="pgaptmp_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198445.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKARALMKDMNMSSGVIKGNEFNQQG"
     gene            1427..1720
                     /locus_tag="ACHKNB_RS25540"
                     /old_locus_tag="pgaptmp_005108"
     CDS             1427..1720
                     /locus_tag="ACHKNB_RS25540"
                     /old_locus_tag="pgaptmp_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198446.1"
                     /translation="MSKVRVIFEFEHVSHDEKPAGNDSVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPVIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            1731..2261
                     /locus_tag="ACHKNB_RS25545"
                     /old_locus_tag="pgaptmp_005109"
     CDS             1731..2261
                     /locus_tag="ACHKNB_RS25545"
                     /old_locus_tag="pgaptmp_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4198447.1"
                     /translation="MVKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIHDWCKTNRNELTNGGETKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGA
                     ESFHVEPFEQDAGLNK"
BASE COUNT          638 a          468 c          626 g          548 t
ORIGIN      
        1 ggcatgggga atcagtggtg aggccgagct ggccgtcatg caggctatcg ctgaaaagcc
       61 gggagcgtta cgcgttctga cacatacgct taaccaggcg tggctcaccg ccagcggtga
      121 aggcgcggcg ctgacagaaa aacatattaa tgcggccttt aaagaggttt ataccaaccc
      181 tgaattactc tcgcaggtgt gattatggct gtatttaata ttcctgatat ttacggacgc
      241 ttttacctgg ttaatttcga taacgtcaaa gcagtctcca tgtctgacag tgaagaatgt
      301 ggcgatttac tctttgaatt taatgatcgc acccgaatgg caatatcggc aggacttgat
      361 cgtgaaggtg cgaaagaagc ttacagcaat atttgtcgct ttatcggtgc aagacgggtt
      421 agttaaacga gaggttatat gaaaatgcag atacagcagg aaatcaatgt tctccacagc
      481 tttcgtgagt ttaacaacaa tgactgtgag gttcgtgatt atatctatga attgatgaac
      541 ggtgacggtg tgtgttttct gtttatcgac ctgtatcaat ggtatgcaga ccagcagtgt
      601 gaatctgtcc ggcagcgttt tgattatgag tgctttgatg tggacgtatg ggaaggcctg
      661 cgttttggtt ttctgatccc gggtgattat cgccgggagt ggcgacatag ttactccatt
      721 cttcgggcaa tcatgcgtgg cgaaacccga aaaatgccgg ggatgggtaa ggttaatcgc
      781 ggcctcctgt tagagatagc agcagcgcac atgaatccat tactgataca accggagttt
      841 acttatgaat ttgtttgatt cactgaataa cgcccgtcgc ctgaccgagc ttgccggtgc
      901 ggtactggaa cgcagtaagc gctatccgca acgttttgca ctgaaaacca cgccgccggt
      961 aggcgaggtt caggggagtg gtgaagaaat tgaaatcacc gttaagacca acggcctgcg
     1021 ccgccgtgtg aaggccaccc gcataagcgg atgcacggtt tactgggaag tatgaagtta
     1081 cttcatggtt cggctctgtc atcaggtcag acgccgggtc agttatccga acaaaacgag
     1141 gaccagagga tgagtaaagt cgtacgcatt attttcgaat acaaggagca cgttatccat
     1201 aaaaacgcgg atggaacagt gcgcatgggg gtaagtctgg acatacgttc aaccgggata
     1261 aagcagaaag gtgatggacc tgccatgatt tttggggtgg ttatgctcgc ggaaagcaga
     1321 gactttgctg aacttgtagc aatgaaagcc agagcgctca tgaaagatat gaacatgagt
     1381 tccggggtta ttaaaggtaa tgaatttaat cagcaggggt aattccatga gcaaagtacg
     1441 cgttattttt gaatttgagc atgtttcgca tgacgaaaaa ccggcaggca atgacagtgt
     1501 tgaagtgcat gaaaagattg gagtggatgt gaaaacagaa cgtgatacgg ataacaggcc
     1561 gacgtcactc tgtgacgttt atgcaagcat tctccagtat cacagtcctg taattattca
     1621 gtttctctca gcggaatttc aggcatctgt acaggctttt ggggcggatg ccatcattaa
     1681 acgccaccgt gtgcataaag catcaggcac actgcaataa ggaaaacaaa atggttaaac
     1741 gtgttacaaa attaaaggcc gcagccgagg ctgcaccgca gacccgcgaa gaagtcagcc
     1801 gcgatatccg caccctgggc gatattcagc gagaggcgct gcgcctggaa acggcaatga
     1861 atgatgaagt ggcagaaatc accgcccgtt atacgccgca gattgaaaac cttaaaaaag
     1921 aaatcaaagt gctttttaaa gggattcatg actggtgtaa aaccaaccgc aatgagctga
     1981 cgaacggcgg cgaaaccaaa actgccaatc tgaccaccgg aacggtgtca tggcggctgg
     2041 gaaatccatc atgcagcgtc agtcgtgatg tggaaggtgt gattgaaatg ctgcgtcgta
     2101 tggggcttga gcgcttcatc cgcacgaaag aggaagtgaa taagcaagct gtcctggcag
     2161 agccggatgc ggtgaaaggg attgctggta ttaaggtgaa taaaggcgct gaaagttttc
     2221 atgtcgagcc ttttgaacag gacgccggac tgaataaata acaccgcatt aaatctttaa
//
LOCUS       DAZFVO020000318         2253 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFVO020000318 DAZFVO020000000
VERSION     DAZFVO020000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.320"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..635)
                     /locus_tag="ACHKNB_RS25550"
                     /old_locus_tag="pgaptmp_005110"
     CDS             complement(<1..635)
                     /locus_tag="ACHKNB_RS25550"
                     /old_locus_tag="pgaptmp_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4198448.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDDSSWASPGTASASETSST
                     AVGAWARAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWAKADALNSVA
                     LGTGAQAMHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTV"
     gene            complement(903..>2253)
                     /locus_tag="ACHKNB_RS25555"
                     /old_locus_tag="pgaptmp_005111"
     CDS             complement(903..>2253)
                     /locus_tag="ACHKNB_RS25555"
                     /old_locus_tag="pgaptmp_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198449.1"
                     /translation="GGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIG
                     FENDPVFRVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPF
                     SVLALPLGSWSAHFAGSKTDAFERILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVHDK
                     GDESRERPVFIIGNDGNNLLAGGKGGDYIEGGGGNDKIEPGKGANRVDGGSGYDTLLL
                     SDVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITD
                     HRYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGN
                     DYLHGASGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHK
                     GEKDFLFLSKDIVTSTDEFISCLKQTGNDVEAGIAGGRIYFVNTALEDINHNSVYIV"
BASE COUNT          660 a          638 c          390 g          565 t
ORIGIN      
        1 accgtgttcc ggataatatt ctccctgttc tctgcagcaa ccgtatcggt atggtcacgc
       61 gccttaagtg cttccgctgc aatccgtcca ctgacgtcac tgtacacctc atccgccttc
      121 ctgttcagtt gcccgacagt ggctgcatcc gtatcccgtg taccatcact gacaccaccg
      181 agaacccggg ttttatccgg tacacgcgga ctggccgggt ttgtgacacc tgagtcatat
      241 ccgatgtaga cttcattttc acgggttgta ccggactgct gaccaagagc cactgaatac
      301 ctgtgcattg cctgtgcacc cgtacccagt gccaccgaat ttaaagcatc agcctttgcc
      361 cagctaccta ctgccacacc attagacgcc ttcggattta cgtaagctga catccccacg
      421 gatgtggaaa acggcgcatg cgcatacgat gcagagccaa gagctgtccc tgcctttcca
      481 gccctcgccc atgcacctac agccgtggag ctggtctctg atgcagaggc tgtgcccgga
      541 gacgcccagg aactgtcatc ctgcgcaaac gccggagcac cggcaatcag aaaggaaata
      601 agcgataaag taataccatg tcgatttatt ttcataaaca acctcattga tatacacaat
      661 atactggcac agagttaaat taatatataa tcaggagaga agtgaaagag tacccggtgc
      721 gaaaatgaat taatctgagc agatttaacg gtaaacgaag cgcaataaat taaaattaaa
      781 aaaacaaaat cacttgcagt ctattatctt tacgttcctc accgcaaccg cacacaaaaa
      841 caaaacacac aatgcattga ttttaaacaa aaaaaacaaa acagaaactc aacacataaa
      901 aatcagacaa tataaacaga attgtgattt atatcctcaa gcgctgtatt aacgaaatat
      961 atccgtcctc ctgcaatacc tgcctcaaca tcatttccgg tttgtttcag acatgaaatg
     1021 aactcatctg tcgatgtaac aatgtccttt gataaaaaca gaaaatcttt ttcaccctta
     1081 tgtttattaa aatccatgat aaatgaattc ccttcacaag cagcatcaaa gacgaagaca
     1141 tcatgaccag taccaccata cagataatca ttacctcccc caccatataa ccagtcgtta
     1201 ccttctgctc cgtacaactc atcattacca gaagccccat gaagataatc attcccgcga
     1261 cctccgtgta aaagacttac tccttctccc ccggcactat gtaatatatc atttccgtca
     1321 agaccaaaaa taatatttcc gaacagacta tcatctccgt ttgtaccttc tgtatgtttt
     1381 ccgtaatcaa catttttatt acgccacttc agaagatacc tgtggtctgt aatctgctct
     1441 tcttctatct gataagggct tagtttactc ctccagtcac cagaaaaaga gataccttca
     1501 atacctgttg cctcagtcag accggctccc cgttctgaat ggaaaaacag cgtcccatca
     1561 ctgaccctgt atatattcca gtcatccatt acatcgctaa gaagcagcgt gtcataccca
     1621 ctgccaccat caaccctgtt tgctccctta cctggttcaa tcttatcatt ccctccccct
     1681 ccctctatat agtcgccacc cttcccgcct gccagcagat tattgccatc attaccgatg
     1741 atgaatacag gtctttcacg actctcatcc cccttgtcat gaacccatga ataccaagat
     1801 gtccagctac tttgctcatc cacaatgact gtactgtccg gtgacatgta gtcgtaaaac
     1861 tgagccccta aaattctttc aaaggcgtca gtttttgaac ccgcaaaatg cgcactccag
     1921 cttcccagcg ggagtgcaag cacagaaaag gggataatat tccagaggaa tgaaccataa
     1981 aggtctgtgt ataaaacaat gttatcagga gaagagtcat attcacgatc cggggttgtc
     2041 aagcccggcc ccattgcttt aactgcctcc cagaacccgg ctccatctcc cagcacacga
     2101 aataccggat cgttttcaaa accgatattt aataccttcc catccccgtc aaataccagc
     2161 ggggaagccc aggctatata gtcagaatta gcataaaaac catcagccag agactcactg
     2221 agtctggcca taatgttagt caacccaccc ccc
//
LOCUS       DAZFVO020000319         2218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFVO020000319 DAZFVO020000000
VERSION     DAZFVO020000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.321"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1393
                     /gene="istA"
                     /locus_tag="ACHKNB_RS25560"
                     /old_locus_tag="pgaptmp_005112"
     CDS             <1..1393
                     /gene="istA"
                     /locus_tag="ACHKNB_RS25560"
                     /old_locus_tag="pgaptmp_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HGU4198450.1"
                     /translation="IKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNK
                     CVTLQLLWEEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPI
                     VGVTDPETGEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELV
                     VPDNLKSATSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERW
                     IMARIRHEIFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEAS
                     YSYTLVKKVRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPR
                     KRTYGYSTRNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYR
                     FCLGLLNLHKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLST
                     MEHENVRGSEYYH"
     gene            1408..2154
                     /gene="istB"
                     /locus_tag="ACHKNB_RS25565"
                     /old_locus_tag="pgaptmp_005113"
     CDS             1408..2154
                     /gene="istB"
                     /locus_tag="ACHKNB_RS25565"
                     /old_locus_tag="pgaptmp_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HGU4198451.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          637 a          530 c          546 g          505 t
ORIGIN      
        1 cataaagcag tggcccttac ctacaggatg ggatgataca aaactaaaac atgcgttcct
       61 tcagacccag gttaagatga agaagcactc tctgcctgac tgggctacag tacaccggga
      121 actgcgtaat aaatgcgtga cgctgcagct actctgggaa gaatactgcg agcgtaatcc
      181 aggcggtttt tacagctata accattactg ccggatgtac cgtgaatggc tcaaaaccac
      241 ttcaccatca atgcgtcagg tacataaagc tggcgaaaaa cttttcgttg attactgtgg
      301 acctatcgtt ggcgttaccg accctgagac cggagaaata agaactgctc aggtcatcgt
      361 agctgttctc ggggcatcaa gttacacatg ggcagaggcc acctggtctc agcagcttga
      421 agactgggtg atgagtcatg ttcgctgctt ccagtggttg ggtggcgttc ctgaacttgt
      481 tgttccggac aatctgaaaa gcgccacatc cagggcatgt aagtatgatc ctgacgttaa
      541 ccctacctac cagcagatgc ttgagcatta taatgtcgca gttttgcctg cgcggccacg
      601 taaaccgaaa gataaagcca aagctgaagt tggcgttcag gttgttgaac gctggatcat
      661 ggcccgaatc aggcatgaga tcttctacag ccttgcatcg cttaatcagc gcattcggga
      721 gttgctggaa agactgaata acaaaataat gcagaagttg ggttattcac gtgcagaact
      781 cttcatccag cttgataaac ccgcactgaa gcctcttcct gaagccagtt acagttacac
      841 cctggtgaag aaagtcagag ttcatgccga ttaccacgtg gaaatcgaca aacattacta
      901 ctcggttcca tgttcgctgt taggccagca actggaagca tggatctccg gagaactggt
      961 aagactcttc aatcaggggc aggaggttgc tgtgcacccg cgcaagcgta cttatggcta
     1021 cagtacccgc aacgagcaca tgcctgaagc tcatcgacag catgccacct ggacgccaga
     1081 gcgtcttctg gaatgggcgg ggcacatagg cagtgaaact catagttatg tgcttcatat
     1141 actgaactct cgtccacatc cggaacaaag ctatcgcttc tgccttggac tcctgaacct
     1201 tcataaaaaa tacagtaaag ccagacttaa tgcagcatgt gcaagagctc tgaaaacaaa
     1261 ggtatggcgt ctgtcaggta ttaaatcgat cctggaaaaa ggtctggata aacaacctgt
     1321 tcaggatcca aaaccagatc tgttatccac gatggaacac gaaaacgtac gcggcagtga
     1381 gtattaccac tgatacggga tccaatgatg aatcatcttt acgaacaact gaccgcactt
     1441 aaactcaccg gcttccgtga tgcgcttaaa aagcaacttg ctcagccggg cacataccag
     1501 gagctgggct tcgaagaacg cctgtcatta ctgacagcag aagaactaac ctgccgtgaa
     1561 aacaggaagg cagagcgtct gatcaaacat gcacggttca gacttaatgc tgagttatca
     1621 aagctggatt atcgtaacaa tagagggctg gacagggccc tcatccgttc actcagtcag
     1681 ggaaactggt taaccctgaa acaaaatatt ttactgaccg gggccaccgg cagcggtaaa
     1741 acgttcctgg catgtgcact tggtcataat gcctgccgac agggatacaa ggtctactat
     1801 tatcgcctta aagcgctgat ggaacagtgc tatcaggggc atgctgatgg aagatacagc
     1861 aaacttttga ccaggctgaa taatagcgat ctgctgcttc tggatgactg ggggctggaa
     1921 cctctctcat cagaacagcg tagcgacctg ctggaaatag tggatctgat gtaccaacga
     1981 ggctcaatca tcgtagtgag ccagttgccg gtggaaaact ggtacaaaat gatcggagac
     2041 tccacacatg cggatgccat cctagatcga ctggttcatg gcagtatcaa gatcgaactt
     2101 aaaggagaat caatgcggaa aatacaatct ccgttgaccg aaggagatca gtgaaggtaa
     2161 tttaaaaacg gttctgtgaa agtgacacga accgatctcc atcgatgtta ctcaccga
//
LOCUS       DAZFVO020000320         2218 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFVO020000320 DAZFVO020000000
VERSION     DAZFVO020000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.322"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..2152
                     /locus_tag="ACHKNB_RS25570"
                     /old_locus_tag="pgaptmp_005114"
     CDS             62..2152
                     /locus_tag="ACHKNB_RS25570"
                     /old_locus_tag="pgaptmp_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198452.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSDVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVSEWRLRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKMKCRHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSMDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWAELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVAVQQPAVDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
BASE COUNT          589 a          541 c          640 g          448 t
ORIGIN      
        1 cctgaagaaa tctggcccag tcgataccag gttaagagct atatgagaaa ggcgtcatga
       61 tatgtatgtc attgcaaaag aactgattgg cgcgcccgga atgcctgcta caacaaaagg
      121 tattcgccag gcattacaac gttacgtaca agggaaaagc tgttgttccc gtcgtcgctc
      181 aggctctaaa gcaactgaat acagtatcga ctgtttacct gaagtgacgc agcaggcatt
      241 acgtgaacgc tacgccctgc aactgatgac gcaaaaagcc gatgaatcac cggctccggt
      301 ggtgacaaag gccagacgct catctgacgt ggttgatgcg gtggaggcat atcgcggatc
      361 accacaactg atggtcgaac gcctcaatgc cctgactgaa aatcagcgcc aggtggctga
      421 tgcacgaatc gcgatcgtca gcgaagttct gaaagtcgcg caacaacctg gtttcagctg
      481 cgcgaaggct atccggttta tcgttgacaa cctggcacgt tcacagctgg acgagcgcat
      541 tgtggcaatg gttgagacgg cgaacgccaa aaagggaaac agccgtgcgt tgagtgaaat
      601 cacgctgaag cgatggattg cggcctttaa caaggcacag aacgccgctg aacgcctgct
      661 tttactggca ccgggtaaac gccaggaaat aaaagccgaa gatattaact ggctgcccga
      721 atttctggcg cagtatcgcc agtcaaacgg ccgaccaatg accgaggctt acgaggattt
      781 tgtttctgaa tggcggctcc ggcacgctga tgagccttat atgctcgata tcatgccctc
      841 ttatgacacc attcgccgcg caatgaagaa actgccggaa gtggtgaaac aaaaaggccg
      901 ggtgaccggc agtgaatacc gtcagcttga gggattcacg cgccgcgact ggtccagaat
      961 gccggtgaat tatgtctgga ttggtgacgg tcacggcatg aagatgaaat gcaggcaccc
     1021 ggttcacggg cggccatttg caccggaagt gacctttgtt atcgacggtg gcacgcgctt
     1081 tgtggtgggc tggagcatgg acctggctga aaatgttttc gccgtagccg gtgccataca
     1141 gcacggtatt cgccatcacg gcaaaccgtt tctgtattac tcggataacg gttcggggga
     1201 aaccgccgac gtcctggata aggaggttgt ggggatactg ccgcgactgg ggattaatca
     1261 cccgaccggg attgccggta atccgcaggg gcgaggcatt atcgaacggc ttaaccgcac
     1321 attaccgatg cgcatagccc gtaaataccg cacctatttc gggaaaggtg cagatcgcga
     1381 gacgttacgc aaaaccaacc gcgatttacg ctcggcattt accgccctgc aacagggcaa
     1441 acggctgaac gcccggcaac agtcagcgat gcgtgattta ccgtcctggg ctgaactgat
     1501 tgatgccatt cgtgacggtg ttgagtggta caacaaccgg ccgcacgatg aattaccgat
     1561 gaagccgaac ggtaaacatt acagcccggc ggagttcaga aaaaaacgcc tggcggaaga
     1621 ggacacggaa attgaatggc tgtccgatgt tgagctgcgg gatatgttcc ggccgatggt
     1681 ggaacgccct gtaagacgct gtgaaatacg ctggctgaat aatatttact acgcgcccga
     1741 gctgcgtgac gagcatggtc gtaaggtgct tatcagctat gacattcatg atgccgaacg
     1801 aattaccgta cgtcgcccgg atggcagcgt gatttgcgag gcggtatggg acggcaataa
     1861 acgcgaagcc ttcccggtca gcgcggaata ctacaaacag cagcagcgcc ttaaaggtat
     1921 gcgtaaacgc gcagaggaaa aaatccgtga tgccgaggat gaggttgtca acgtgctgga
     1981 gcacaagccg caggagccat ggctggaaaa catctaccgc ccggtgggta atacggtggc
     2041 cgttcagcaa cctgccgttg atgatgaacc tgatgaagaa tacgagcgta atttccagcg
     2101 gggattgcag ttgctggaag cgaaattaaa agaaaatgac ccgctggcct gaaataaaaa
     2161 aataacccga gcggcgactc aggttatttg attaaacaag gtatcaaatg agaggtta
//
LOCUS       DAZFVO020000321         2216 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFVO020000321 DAZFVO020000000
VERSION     DAZFVO020000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.323"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..636
                     /locus_tag="ACHKNB_RS25575"
                     /old_locus_tag="pgaptmp_005115"
     CDS             <1..636
                     /locus_tag="ACHKNB_RS25575"
                     /old_locus_tag="pgaptmp_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4198453.1"
                     /translation="EPDVAGAMYLKSEDGQDWYECQALFSPETLKVVYDSRGVITGYG
                     KETALLWPVNQSVAEVPDTPESRKIDLSGRWGFDGEKITDLLTAEKARGMKGDEINAW
                     RNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDAENNDVEVTA
                     EELIALSEAAEQAMFTKGMEIHVRQRTMKKVLEKLTSADEILAYRVGRAQE"
     gene            1174..>2216
                     /locus_tag="ACHKNB_RS25580"
                     /old_locus_tag="pgaptmp_005116"
     CDS             1174..>2216
                     /locus_tag="ACHKNB_RS25580"
                     /old_locus_tag="pgaptmp_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198454.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYA"
BASE COUNT          561 a          483 c          657 g          515 t
ORIGIN      
        1 gagccggatg ttgcaggcgc gatgtacctg aagtctgagg atggtcagga ctggtatgag
       61 tgtcaggcgt tgttttcgcc ggagacgctg aaggtggtgt atgacagccg gggtgtgatt
      121 acgggatacg gtaaggagac tgccttgttg tggccggtga accagagtgt ggcagaagtg
      181 ccggataccc cggagagccg gaaaattgac ctgagcgggc gctggggctt tgacggggag
      241 aaaatcacgg acctgctgac cgcggagaaa gcgcgcggga tgaagggcga tgaaattaac
      301 gcctggcgta atgcgatgga agcggcgaac tacacgtttg agcacaatgg gcgtaaatgg
      361 gactacggga agtcaacgca gacgcgtctt gagccgtcgg tggctgcagc gaaagcgggg
      421 aaactgccgg aggcgttttt ctggacggat gcggaaaaca atgatgtgga agtgacagca
      481 gaagagctta tagcgctgag tgaagcggca gagcaggcga tgttcaccaa agggatggaa
      541 atccacgtcc ggcagcgcac catgaagaaa gtgctggaaa agctgaccag tgcggatgag
      601 atactggcat acagggttgg cagggcacag gaataacaga caaaaagatg ggggaccatc
      661 acctccccca tgaactgagt caattataca cttgtgaagt atttactatg tgaataaata
      721 ctgtcacgga aagtgcaggc ctggatgtga tggaaatcat gtttccggcg catcctgttg
      781 atatggataa tttttcagat ttccggaagt gagagttttt ggtgtttcca taccagccac
      841 agttgatgtg tgttgtggcg cttggcagtg cgattaaagg agcagaggga ataaaaaacg
      901 ggggcatgac caacccccgg tacaaggaag gagtaactac aatggaaaaa ttaatgaaga
      961 cctttagtgc tacgttatat ggcgaataaa gactgccaca gggaagtttg cagaaacagt
     1021 gatgccgatc gtgtttctga cgctcttctc tgataagagt aatttttcag atttctggaa
     1081 atctgtattc tgcacaggcg caattgtatg ccgcctttaa aacttcaatt cagtgactca
     1141 cctgccattc aaattttcgg ataccagaca accatgcctt atatcgatat aacaactatg
     1201 cgcgggatga tgccaggcgt tattgcatct atgctgccag atcattctgc tgtactggca
     1261 gaaaactgtc attttcgcta tggagtgatc acgcctgaac accagatgtc agaggctgag
     1321 aaaacattcg cgattaagcc gaaaaccatt tttcattacc gtgacgattt ctggtttgca
     1381 tggacggatg tggtggatgt gatccgcagt ccggtcgctc aggactccca cgggcgtatt
     1441 tactacactg acgggcgttt tcctaaagtg acggatgcga ccattgccac aaaaggggac
     1501 gggaatcacc cgacatcatc gtatcgtctg gggatccccg cgccgacgac agcacctgtc
     1561 tgtactgttc agcagggcgg tgatgtttct gacgataacc cgaatgatga cgaaacccgg
     1621 ttttatacgg aaacctttgt ctcagattat ggtgaagaag gtccgccagg tccggcgtct
     1681 ctggaggtaa cactccgtac tccggggact gcggtacagc tgacgctgtc tccggtgcca
     1741 ttgcagaatg ccagtattaa acgccgccgg atttatcgct ctgcatcagg tggaggagaa
     1801 gcggattttt tacttgtggc tgaactggat gcatccgtgc tcagttacac ggacaaaata
     1861 ccggggaaaa accttggacc ttctctggcg acatgggatt acctgccgcc gccagagaat
     1921 atgacaggcc tttgcctgat ggctaatggt attgccgccg ggtttgccgg taatgaagtg
     1981 atgttttcgg aagcgtatct gccgtatgca tggccggaag tgaatcgtca cacgacggca
     2041 gaagatattg tggctatctg tccgctggga acgtcactgg tggtggcgac aaagggggag
     2101 ccctatctgt tcagtggggt atcgccttcc acaatttctg gctccagaat tccttccatg
     2161 caggcatgcc tgagccgaag aagtatggtg gcgatggagg gattcgtact ctatgc
//
LOCUS       DAZFVO020000322         2175 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFVO020000322 DAZFVO020000000
VERSION     DAZFVO020000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.324"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..156
                     /locus_tag="ACHKNB_RS25585"
                     /old_locus_tag="pgaptmp_005117"
     CDS             <1..156
                     /locus_tag="ACHKNB_RS25585"
                     /old_locus_tag="pgaptmp_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198455.1"
                     /translation="KRYPQRFALKTTPPVGNVQGTGEIEITIQTNGLRRRVKATRISG
                     CTVYWEV"
     gene            158..439
                     /locus_tag="ACHKNB_RS25590"
                     /old_locus_tag="pgaptmp_005118"
     CDS             158..439
                     /locus_tag="ACHKNB_RS25590"
                     /old_locus_tag="pgaptmp_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HGU4198456.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLQVPGIPEAVTEEEAFNTMIRFCREVRRQVSYPNRTRTRG"
     gene            436..708
                     /locus_tag="ACHKNB_RS25595"
                     /old_locus_tag="pgaptmp_005119"
     CDS             436..708
                     /locus_tag="ACHKNB_RS25595"
                     /old_locus_tag="pgaptmp_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198457.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            713..1006
                     /locus_tag="ACHKNB_RS25600"
                     /old_locus_tag="pgaptmp_005120"
     CDS             713..1006
                     /locus_tag="ACHKNB_RS25600"
                     /old_locus_tag="pgaptmp_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198458.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            1018..1548
                     /locus_tag="ACHKNB_RS25605"
                     /old_locus_tag="pgaptmp_005121"
     CDS             1018..1548
                     /locus_tag="ACHKNB_RS25605"
                     /old_locus_tag="pgaptmp_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4198459.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            1646..>2175
                     /locus_tag="ACHKNB_RS25610"
                     /old_locus_tag="pgaptmp_005122"
     CDS             1646..>2175
                     /locus_tag="ACHKNB_RS25610"
                     /old_locus_tag="pgaptmp_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198460.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGSLPAGWRKRDDVAFIGGGPCFIARPDESCPEGPAIAAMVEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNTDSWWTPSLSRDALCVVFKVGAYGREING
                     CIPEECQEIKHSEYVAL"
BASE COUNT          603 a          461 c          607 g          504 t
ORIGIN      
        1 aagcgctatc cgcaacgttt tgcactgaaa accacgccgc cggtaggcaa cgtgcaggga
       61 accggtgaaa ttgaaatcac catacagacc aacggcctgc gccgccgtgt gaaggccacc
      121 cgcatcagcg gctgcacggt ttactgggag gtgtgaggtg aaaaaaaatc tcattgcatg
      181 ggcgtggtcg agtggtctta ttgagtttgg ttacgtcctg ccggaaggtg cattgccgat
      241 agttgccgga aagcctgcca cggtacggca tgtgattgag gttatggcgc gtcatggacg
      301 tgatgaacag gagcagttac aggttccggg gataccggaa gcggtgacgg aggaagaagc
      361 ctttaatacc atgattcggt tctgccgtga ggtcagacgc caggtcagtt atccaaacag
      421 aacgaggacc agaggatgag taaagtcgta cgcattattt tcgaatacaa ggagcacgtt
      481 atccataaaa acgctgatgg aacagtgcgc atgggggtaa gtctggacat acgttcaacc
      541 gggataaagc agaaaggtga tggacccgcc atgatttttg gggtggttat gctcgcggaa
      601 agcagagact ttgctgaact tgtggcaatg aaagccagtg cgctcatgaa agatatgagc
      661 atgcgttccg gggttattaa aggtaatgaa tttaatcagc aggggtaatt ccatgagcaa
      721 agtacgcgtt atttttgaat ttaagcatgt ttcgcatgac gaaaaaccgg caggcaatga
      781 ctgtgttgaa gtgcatgaaa agattggtgt ggatgtaaaa acagaacgtg atacgaataa
      841 caggccgacg tcactctgtg acgtttatgc aagtattctc cagtatcaca gtcctgaaat
      901 tattcagttt ctctcagcgg aatttcaggc atctgtacag gcttttgggg cggatgccat
      961 cattaaacgc caccgcgtgc ataaagcatc aggcacactg caataaggaa aaacaaaatg
     1021 gcaaaacgcg ttacaaaatt aaaggccgca gcagaggcgg caccgcagac ccgtgaagag
     1081 gtcagccgcg atatccgcac cctgggcgat attcagcgag aggcgctgcg cctggaaaca
     1141 gcgatgaatg atgaagtggc agaaatcacc gcccgttata cgccgcagat tgaaaacctt
     1201 aaaaaagaaa tcaaagtgct ttttaagggg attcaggact ggtgcaaaac caaccgtgat
     1261 gagctgacga acggcggcaa aaccaaaact gccaatctga ccaccggaac ggtgtcatgg
     1321 cgactgggga atccatcatg cagcgtcagt cgtgatgtgg aaggtgtgat tgaaatgctg
     1381 cgccgtatgg gccttgagcg ttttatccgc acgaaagagg aagtgaacaa ggaagcggtc
     1441 ctggcggagc cggatgcggt gaaagggatt gccggtatta aggtgaataa aggcgctgaa
     1501 agtttttatg tcgagccttt tgaacaggac gccggactga ataaataaca ccgcattaaa
     1561 tctttaaata tcacttcgtt ttaattatgg cgctcgcgtc aggggactgc ctgcgcctgt
     1621 aaactgaaaa taaggaacag gaaatatggc atatttttat ttcaaattag accgtgtaca
     1681 gacaaataaa tatttcacca aatatcaaca gactgtttta ccgctacgca acagtattct
     1741 tcgggcatta ctgaaaaata caggcgctgc cggattgcgc ttaaagccgt tcgccatgga
     1801 cgtaatcagt gagttttatt tttctggttc tctgcctgcg ggctggcgta agcgcgatga
     1861 tgtggctttt atcgggggcg gaccgtgctt tattgccaga cctgatgagt catgccctga
     1921 aggtccggcg attgccgcaa tggttgaagc cgctgaacgc gagttaagaa agcgtcctga
     1981 tttccttgtc tggctctgtg aaaagctggg ggtaatgaga atcccctcca tgtttaacac
     2041 ggactcctgg tggaccccgt cactctcccg tgatgccctg tgcgtggtgt ttaaagtagg
     2101 cgcttatggc agggaaataa acgggtgtat tcctgaagaa tgccaggaaa ttaaacattc
     2161 tgaatatgtg gcgct
//
LOCUS       DAZFVO020000323         2134 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFVO020000323 DAZFVO020000000
VERSION     DAZFVO020000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.325"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..135
                     /locus_tag="ACHKNB_RS25615"
                     /old_locus_tag="pgaptmp_005123"
     CDS             <1..135
                     /locus_tag="ACHKNB_RS25615"
                     /old_locus_tag="pgaptmp_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198461.1"
                     /translation="HKFRQFRYIRFLNKRTRKRLNTKLFKVQPYPKPQTVKTGLKESE
                     "
     gene            complement(264..2134)
                     /locus_tag="ACHKNB_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /pseudo
     CDS             complement(264..2134)
                     /locus_tag="ACHKNB_RS25620"
                     /old_locus_tag="pgaptmp_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
BASE COUNT          601 a          513 c          445 g          575 t
ORIGIN      
        1 cataagttcc gacagtttcg ttacatccgg tttctgaaca agaggaccag aaaacgcctc
       61 aacacaaagc tattcaaagt ccagccttac ccaaaaccac aaacagttaa aactggtttg
      121 aaagagagtg aatgagcgct taaaaactca ttctaaaacc caaaagaaaa gcgccgattt
      181 agtatcaaat aagttgcgta tcggcgtttt tcagcggtat caaatgaaat gcaaatcggt
      241 atcaaaaaaa tcgccgcgct ataaccactg gctcccaaat taacgccata gcgactgaca
      301 gccagcgtag agctataagt gcccgtaaag gccgaaagat gatcatgatt cttacggtca
      361 taactatcag atacggtagc gtagacattt ccgtattgcc catcccaacg cccgttaacc
      421 gcgctgttta tggtatcggt gttcaggcct ccgacggtta cgccaagttc tttatggctg
      481 aatgaatcgt tataaaaggt atgcgataac tgccatgacg tttgatcgcc atcctgttcg
      541 ctataacgat aatccgtaga cacatttgtt gaatggctgt tgttattgct atccatcgtt
      601 ggcgtgtcag ataacgaaaa cgataagtac aagccgttgt cgtcgttatc gccaccgttg
      661 tcattacgat ggaaaacacc gactgacgta ctcaaaatcc agtcacgcat cacaaaagcg
      721 cgggaagcgc taagttgtac agtttcagag atactttgcg ctgttgtctg gcgaagatag
      781 ttattttcaa agctctgctg tttatcccaa cgcaaactat catcagtgtt attttgtgtg
      841 cgggtataac ccagcatcat attccagccg acaaaattct gtgaaaccgt cgcggaaata
      901 ctttcataac agcttaaggc gttatagctc tctctgctgt cggttgtaca agcatcgccg
      961 tcagagttgg tccgataaaa gcccaacgtc ggccatcccg gattactcca gttggcctgt
     1021 tgcatatcac ctttgccgcc gtcatcgtta cggaacacgc tggcgctgat tgcaagattc
     1081 cccaccccgc ctaaatctgc cgtccagtta ttacctaact cgaaagcact cacatcatcg
     1141 gcattcgcca gccctgcgta gagctcatat tgcggatgta atggcaggcg tactcctagc
     1201 tgataagctg aactttcatc atcagaaacc tgtgatgtag ttttacctgc ctgtaagaac
     1261 cattgcgcat ttccgtcagt cagaccgccg gttttggtaa acggcacaag ctcggtgcgg
     1321 gtgagttggt tattttcgta gacttttaat gccacgctgt agctgcctgg cggaaaggaa
     1381 ctggtatcaa taaattgcga accactattg agataaaacg atcccaaaag ttgctcatta
     1441 cgataagcgt caacacgaga attgcgcgaa agcagaacca ttactggggt tccttgctgg
     1501 ctttgcgcct ggtttaaata gctcagtgtc gacccgatac gcatcccatc aattgcaccg
     1561 agtggcagaa agttaaaggt aaagttcccg ccttgtgcat taaatagtgt gcggttgtcc
     1621 atgcgacccg cctgcacata ataacgacgt aaaaaatcat aacgataata gagatcgctg
     1681 acgtcagcat tactgtcact gacatcatct gcatcataac cgttgaaatt ccagtgtgca
     1741 ccaatataac tattttctgt tattcccagc gcaccgtttc cctgaataga caacgattgg
     1801 taatcatcct gtgccagaac attgatgtct tgctgatgta taaaagcatt aacagtattt
     1861 ttgtctggat ttaaatataa tgacttcgca tcgaaagctg aattccattg cggattaata
     1921 aacaacgttg ccgaactttc ttcatcatta tagattattt ccagcgtatc ggtattcaga
     1981 aaaccacacc ctgagtcagt acgtacataa ccgcaagcca attcaccgtg gcgagctagc
     2041 ggctgcgata atttttcttt cactatatgc gcgattttct gatcgtctac atccagttcc
     2101 agcttgttat atagacttgc tggatcaaga aatg
//
LOCUS       DAZFVO020000324         2113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFVO020000324 DAZFVO020000000
VERSION     DAZFVO020000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.326"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            7..771
                     /locus_tag="ACHKNB_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /pseudo
     CDS             7..771
                     /locus_tag="ACHKNB_RS25625"
                     /old_locus_tag="pgaptmp_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1117..1275
                     /locus_tag="ACHKNB_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /pseudo
     CDS             1117..1275
                     /locus_tag="ACHKNB_RS25630"
                     /old_locus_tag="pgaptmp_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1499..1618
                     /locus_tag="ACHKNB_RS25635"
                     /old_locus_tag="pgaptmp_005127"
     CDS             1499..1618
                     /locus_tag="ACHKNB_RS25635"
                     /old_locus_tag="pgaptmp_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HGU4198462.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1683..>2113
                     /locus_tag="ACHKNB_RS25640"
                     /old_locus_tag="pgaptmp_005128"
     CDS             1683..>2113
                     /locus_tag="ACHKNB_RS25640"
                     /old_locus_tag="pgaptmp_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HGU4198463.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNS"
BASE COUNT          637 a          376 c          467 g          633 t
ORIGIN      
        1 tgaaaaaaaa tagatgagat ttcatctttg tcagagtcaa aggaacaccc catagatatt
       61 caagaaaaaa aagatgcgtt tgtgaatgag ttcaagggga tattatttga taaaaatgga
      121 aggtcttcag agtttctact taatttttat gaatgttgct atgagttttt accaagagcg
      181 cagcctcagg ataaaatcga aagctataat tcagcactgc aagctttctc catcttttgt
      241 tcatctacgt tgatacataa taatataggc tttgatttca aattatttcc agaagtcaaa
      301 ctgtgtgggg aacatcttga aacggtattc aaatataaaa atggcgatga tgtccgggag
      361 atagccaaaa ttaacattac tctccaaaaa gaagaggatg gtttatataa tttaggtgga
      421 ttggatttta agggatgctt cttttctgga cagaacttca gtaactatga tattcaatat
      481 gtgaactggg gaacgtcatt gtttgatctt gatactccgt gtatttttaa tgcgcctgct
      541 tacaacaaga gtaatgaaaa atcattaaaa cctgtgagcg aaaacggttt aagtggagtc
      601 ttgactgatc gtaataataa aataaaactc atcacgggcg tggcaccatt cgatgatatt
      661 ttatttatgg atgatgactt tgatgatagt tcctctgagg atgatcccgt tgagaatagt
      721 cctgttgtga ctagtcccgt tgtatcaagt tctaaaagca gttttcaatg attaataaat
      781 ggctattttt acaaagagat aataacgtag attgaaacaa tatgtattat tgattgagta
      841 aacgggatta ttcttcacac tacatccatt ttagtgtcac taccaaagaa aaaggggcta
      901 cgttttcatg taaccccttg atttatttgg tgggctggca gtgactgaat tggtgctgta
      961 attggttgaa atatttcatc taatgtctgg ttcaactttc cttagctgcc tgaaagtttt
     1021 ttgatcttac ccagcaataa cagcgttacg gtgcaccacg cctgactgaa gaactgcgtg
     1081 ctcagggtta ccacttcaac gtaaaaccgt ggcaggaggt ccggactgaa aaaactggtc
     1141 aggacatcat cggcagggaa cgcactgcta gtctgtaaac tgaaccgact ggggcgcagt
     1201 atgtggcatc ttgtcgtgct gttggaggag ctgtgcgaac gtggtattaa ctttcgtgct
     1261 ctggcccaat ctatatttgc ccaacaatgg ggggacgaat gctgtaaaag taaaacaatc
     1321 tgcgatctca aagttattgt gtgattttta tgtgagcaga agatatttat cagaaacgat
     1381 tatgtaaatc attttatttt gccaacggcc tcattgtcga aagaaaagaa tacgcccgta
     1441 tttcctgaat ggattgactt ttttatccaa ccacacttca gcgcactgcg tttaaaaaat
     1501 gcctcattct tatgcggaat atcatcattt catcatgatg tctttgatga gcggtgaaca
     1561 caatacactt gcgctgtctc ttcaggatga atcccctggt ctggtgcctg tgggctgatg
     1621 ttgcagcaaa gctaaggtcg cttaaacgct actcagtatt cacttttcag aggatgaaat
     1681 ttatgaacag gaccagtccc tattattgtc gccgctcagt actttcctta ttgatatctg
     1741 ccttgatata tgccccgcct gggatgacgg ccttcactcc tgatgttatt ggtgtggtaa
     1801 acgatgagac tgtagatggc agccaaagag tagatgaacg aggtacaaca aataacactc
     1861 atattatcaa ccatggccag cagaatgttc atggcggggt atctaatgga agtcttattg
     1921 aatcgggtgg atatcaagat gtaggaagtc ataacaattt tgtggggcag gctaataata
     1981 caaccattaa cggaggaaga cagacaattc atgacggggg catttccacc ggtacgataa
     2041 tcgacagtgg caatcaggac gtttatacag gtggtatcag caatggaacg acaattaagg
     2101 gtggtaattc aca
//
LOCUS       DAZFVO020000325         2072 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFVO020000325 DAZFVO020000000
VERSION     DAZFVO020000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.327"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            78..410
                     /locus_tag="ACHKNB_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /pseudo
     CDS             78..410
                     /locus_tag="ACHKNB_RS25645"
                     /old_locus_tag="pgaptmp_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(701..1174)
                     /locus_tag="ACHKNB_RS25650"
                     /old_locus_tag="pgaptmp_005130"
     CDS             complement(701..1174)
                     /locus_tag="ACHKNB_RS25650"
                     /old_locus_tag="pgaptmp_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198464.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRVILAREFFIPENRKSVPTHD
                     ESWVIICDAMVSRLMRCMFQGRRYLQLDAEEITGRLETDRKIRNGDWEQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGIHTKTVNTFLYMGLGKNGLRYRSVKHLVGRA"
     gene            complement(1262..>2072)
                     /locus_tag="ACHKNB_RS25655"
                     /old_locus_tag="pgaptmp_005131"
     CDS             complement(1262..>2072)
                     /locus_tag="ACHKNB_RS25655"
                     /old_locus_tag="pgaptmp_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4198465.1"
                     /translation="AKDYNIAISNGMSSEAALTAAKDGNGALLQEFNRLGTNVNDLKN
                     ATTFALDENGEITYEKGVESIPVKDVVAEVKADTTIRKNKDGSYTLDQTAPDTVRVNA
                     AIVDLDKNLKDRTTVGVNSDGSLTTAEGATKTVAVSEGLVSLSGRTDRIDAAVGGIDT
                     RVTKNTQTIQNHSRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGK
                     FNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGIAASDGDVSYNVGVNFEF"
BASE COUNT          492 a          573 c          418 g          589 t
ORIGIN      
        1 gaccatctta cttttttctt tctgataaaa tattgtctgt tatccattat cattactaat
       61 acttgcgtag tgaatttatg aacaatccta atccatgcgt gatgtgtggt gcctgttgtg
      121 catttttccg cgtctctttt tactgagctg aagcagatga tgctggcggt accatccccg
      181 taacactgac agaacagatt tctccgttcc atcgatgcat gcagagcact aaccagaaaa
      241 atcctcgctg tgtggtcctg agcggtcaac cgggagtcaa tgcctgctac agcatttatg
      301 aaaatcgctc atccacctgc agggaatttc ctatgtccgg cgacaatggt gtggttaatg
      361 aagcatgtaa ccgtgcgcga gcaaaatacg gcctaacccc actttactaa acgcgatcgg
      421 tatcactgtc atgacatatt ttgtatgcca ttattctgac gacttaacaa ctcacttatt
      481 gtaatcaagt ttctttcttc tggtcgggga gctgtgcagc tccccttttt tattgcatca
      541 ttaaggcagc cactgcacag agcgttacca ccgtacccag ccacacaaaa agcatgaaac
      601 acatcgctcc tgctgtgtag cgcttcttta cgtatttcag atttgcctgt ttcatgacaa
      661 tttaccagat tattcttcac ccccggataa caaaacgcgg ctatgcccgc cccacaagat
      721 gcttcacact tctgtacctc aggccgtttt taccaagccc catatacaga aaggtgttca
      781 ctgtcttcgt gtgaattccc atttcacggg caagatgaca cggctttgac tcccggtata
      841 cataaccaaa catcaccacc atctctgaca tcgtcagcgg acgtgcgtat gtgttctgtt
      901 cccagtcacc gttacggatt ttcctgtccg tttccagacg ccctgtaatc tcctccgcat
      961 ccagttgaag ataacggcgt ccctgaaaca tacagcgcat cagacgactc accatagcat
     1021 cacagataat cacccaggac tcatcatggg tcggtacact cttcctgttc tccgggataa
     1081 aaaattccct cgccagtatc accctgtaac cttccagcat ctgttgcaga ccgtactcca
     1141 gataacggtt acgggtcgcc agaatcactt tcatcgcagc cccctgtttc atgctcacat
     1201 gagaaaaaaa cggcaggcca aatcccctgc ctgccgtcat gcttcatgtc actgtccgca
     1261 attaaaactc gaagttcaca ccaacgttgt aggatacatc accgtcgctg gctgcaatac
     1321 ctgctttggc tgctgtctgc tcgttaaagc ggtaacccac accaacggcc acagcctgtt
     1381 tgtcactgta accacccagt gctgccgtgg cgttgaattt acccacactg tacggctgga
     1441 acaaacctgc cagtgccgca ctctgtgcag cagcacgttt catttcttcg tggttttcac
     1501 gaatctgacg ctgctgactg ttcagccgcg cgttatgctc ctgcagctgg cgggagtgat
     1561 tctggatagt ctgagtgttt ttggtgacac gggtgtcaat gccaccaacc gcagcatcaa
     1621 tacggtcagt tcttcccgac agggaaacaa ggccttcact taccgcaaca gtttttgtgg
     1681 ctccttcagc agtggtcagg ctgccgtctg aattcacgcc aacagtggtt ctgtctttca
     1741 gatttttatc cagatcaaca attgcagcgt tcacacgaac agtatcaggc gcggtctgat
     1801 ccagggtata gctgccatct ttgttcttac gaattgttgt atcggctttt acctctgcaa
     1861 ccacatcctt aacaggaatg ctttccactc ctttttcata ggtaatttca ccattttcat
     1921 caagagcaaa tgtagtggca tttttcaggt catttacatt tgtgccgaga cggttgaatt
     1981 cctgtaacag cgctccatta ccatctttag ccgctgttag cgcagcttct gaactcatgc
     2041 cgttactgat agcaatattg taatcttttg ca
//
LOCUS       DAZFVO020000326         2071 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFVO020000326 DAZFVO020000000
VERSION     DAZFVO020000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.328"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..78
                     /locus_tag="ACHKNB_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /pseudo
     CDS             1..78
                     /locus_tag="ACHKNB_RS25660"
                     /old_locus_tag="pgaptmp_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            474..1013
                     /locus_tag="ACHKNB_RS25665"
                     /old_locus_tag="pgaptmp_005133"
     CDS             474..1013
                     /locus_tag="ACHKNB_RS25665"
                     /old_locus_tag="pgaptmp_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HGU4198466.1"
                     /translation="MAAIKMGVLMNNQLSEICSDSIVSMVKKGVRRFKRPEVGDLFTL
                     EMQDIGFIHGMVARNDLEFVKGQADFNIIYIYKEITKNQSDNLSLAKSELMFPPFIVN
                     DMAWRQGYFQTYDSIPISEIDVFNEYCFYSKAKKQYENERWESCKAFEPCSDLVLSSS
                     LTIAIKIYMRLNPKMKILD"
     gene            1086..2071
                     /locus_tag="ACHKNB_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /pseudo
     CDS             1086..2071
                     /locus_tag="ACHKNB_RS25670"
                     /old_locus_tag="pgaptmp_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          584 a          432 c          573 g          482 t
ORIGIN      
        1 ctggggttgc aggggggtga gcagacttac cggtatgtgc ctaatccgct ggggtatgtt
       61 gatccgttag ggttggctgt atgtcctaaa ttgtatgaaa tatacaaggg attaagagca
      121 caaggattta acccagcaga agctagagat ctagcaaaat ggttcaatag tagctgggga
      181 aaggctgatg tatatcttcg ttcagcaata ggatcaggaa aaatagatta tgttggaata
      241 agtagaaatg tgcttaatcg ttatccgcaa ggtaaatatg atttggagtt actcacaaga
      301 ctaacaggac aacttccgag gaatcaagca agagcaatag aacaagctat cattaatgct
      361 aaaagaggaa acggcttcac aaatatccag aatagtatag atcctgctaa agatcatttt
      421 gatgcagcgg taaaatgggg tgaggagtgg ttaaagagta atggatttgg tggatggctg
      481 caataaaaat gggggtatta atgaataacc aattaagcga aatatgttca gactccattg
      541 tttcaatggt taaaaaagga gtgcgaagat tcaagagacc tgaagtgggc gatcttttta
      601 cactagaaat gcaagatata ggttttattc atggcatggt tgctcgaaat gaccttgagt
      661 ttgtaaaggg acaagctgat tttaatatta tttatattta caaggaaata acaaaaaacc
      721 aaagtgataa tttatctttg gcaaaaagcg aacttatgtt tccacctttt attgttaatg
      781 acatggcatg gcgacaagga tattttcaaa cttatgatag tattcctata tcagaaattg
      841 atgttttcaa tgagtattgt ttttactcaa aagcaaagaa acaatatgaa aatgaacgat
      901 gggagtcttg taaagcattc gaaccatgtt ctgatttagt tttgtcgagt tctttgacaa
      961 tagcgataaa gatttatatg cgtttaaatc ctaaaatgaa aatattggat taatctgtgt
     1021 cgttcagact ctatattgag ttattgcgaa ccgggtgttg caggtgcacc cttccgtgac
     1081 cctgcaaccg ccacggcaac ctgctggcct ggacggacgg caacggtgtt gtctggacga
     1141 tggaatacgg cccgttcgat ttgccggtgg cgcgaacgga cggtgaaggc caccgctggc
     1201 agtaccgcta cgataaagac acgctgcaac tgacagaagt cattaacccg cagggcgaat
     1261 cataccttta tgttctggat aactgtggcc gggtgacgga agagcatgac tggggcggcg
     1321 tggtctggcg ttaccgctat gacgccgatg gcctgtgtac cgccagggtt aacggcctgg
     1381 aggaaactat cctctacagc cgggacgccg caggccgcct ggcagaaatc atcacgccgg
     1441 aaggcaaaac gcagtatgcg tatgacaaat ccggcaggct gacgggtatc ttcagcccgg
     1501 acggtacatc acagcgcacc ggctatgatg aacgcgggcg ggtgaatgtc accactcagg
     1561 gccgacgggc cattgaatac cactaccccg atgaacacac cgtcatccgc tgtatcctgc
     1621 caccggaaga tgaacgcgac agacaccccg gtgaatccct gctgaaaacc acgtaccgct
     1681 acaacgccgc cggagaactg acggaggtta tcctgccggg ggatgagacg ctgacgttca
     1741 gccgtgatga ggcaggacgt gaagtgctcc ggcacagtaa ccggggtttt gcctgtgaac
     1801 agggctggaa tgcagccggt cagcctgtca gtcagcgcgc cggttttttc ccggaagaag
     1861 ccacatgggg cgggctggtt ccgtcactgg cgcgggagta ccgttacgac agcgcgggta
     1921 atgtgtcagg cgtcaccagc cgggaagatt acggacggga aacccggcgg gaataccggc
     1981 tggaccggaa cggccaggtc acggcggtga cagcctcagg caccgggctg ggctatggcg
     2041 aaggcgacga gacttatggc tatgacagct g
//
LOCUS       DAZFVO020000327         2058 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFVO020000327 DAZFVO020000000
VERSION     DAZFVO020000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.329"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..136)
                     /locus_tag="ACHKNB_RS25675"
                     /old_locus_tag="pgaptmp_005135"
     CDS             complement(<1..136)
                     /locus_tag="ACHKNB_RS25675"
                     /old_locus_tag="pgaptmp_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4198467.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     Q"
     gene            203..730
                     /locus_tag="ACHKNB_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /pseudo
     CDS             203..730
                     /locus_tag="ACHKNB_RS25680"
                     /old_locus_tag="pgaptmp_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            727..1713
                     /gene="ycdU"
                     /locus_tag="ACHKNB_RS25685"
                     /old_locus_tag="pgaptmp_005137"
     CDS             727..1713
                     /gene="ycdU"
                     /locus_tag="ACHKNB_RS25685"
                     /old_locus_tag="pgaptmp_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HGU4198468.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1906..2042)
                     /locus_tag="ACHKNB_RS25690"
                     /old_locus_tag="pgaptmp_005138"
     ncRNA           complement(1906..2042)
                     /ncRNA_class="other"
                     /locus_tag="ACHKNB_RS25690"
                     /old_locus_tag="pgaptmp_005138"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT          481 a          421 c          512 g          644 t
ORIGIN      
        1 gctgtgattc atacaggctg agttcacggg ctgcggcagt aacaccgatg cgttcagcaa
       61 gcttcagggc ttcactgcga aattcaggcg aatgctgttt acggggtttt ttactggttg
      121 atactgtttt tgtcatgtga gtcacctctg actgagagtt tactcactta gccgcgtgtc
      181 cactattgct gggtaagatc aggcggttaa aggcatcaac cgcgaacaac tgcagacgat
      241 gattacggaa tcaggcattg cgctggatat tagctgtgat aacacgccgc gccagcaggt
      301 tattggggga acgcaggctg cgttgaatga atttgccacg ctgctgatgg ccgctggata
      361 cgaaccggtt aaactcggtg tgagcggtgc ctggcacacg cgtttgatgg aagacggcgt
      421 tcaggcaatg cgcgattatc ttgccggact ggatatcgct tcgccagagc atcaggttct
      481 gatgaacgta acagcaaaaa gcgaggtcgc tccgtcaatt atcaaagaaa atctctcatt
      541 acacttaact catacggtga aatggactga atctttcgat acatttctga atatgccaac
      601 cccggtagct ttccttgaaa taagtaataa gccctatttg gggaatatgt taaatgattt
      661 tgctggcgtg gatcagcaac gagttatgca ttgtcgtaaa gcatttagtg atgcaaaggt
      721 attcaaatga ttcctgatta tttaactttt attcgctttc aggataaacg aaatctgata
      781 tacatttatg ctattggact tattctgata ggcttttatt ggaagaatgc agggtttact
      841 tttccatcag aggatattgg tgtagttagt gggattctgg ccctagtgct gtataatttt
      901 atttttgatc tcaaggcgta ctgggcttat aaatgcgtca cgaagaatat cgatttttcg
      961 tggtttaaga aaaagcagaa ccacaaaata gaattatttc ttacacaacc tctggtggca
     1021 ggatttctgt cgttaatcat gttgagtgca atgagttggg ggctatacca gcttctaccc
     1081 tcgttatatg cgctgttcct gatttcgtta cttgggccgt tggtcatctt tctgctgttt
     1141 cggatgatcc gcaccagtta tgtcaagcag gtcgctattt cagtagcgaa aaaagtaaaa
     1201 tataaaagtc tgactcgcta tgtgctgctt tcggtgtgca tctcaacggt tgttaacctg
     1261 cttactatca gcccgttgcg taacagtgat tcttttgtga cagaggggca gtggttaacg
     1321 tttaaatcga taattgcatt gctcattctt tgtggcgtag tgttggcgat taatctgttt
     1381 tttctgcgct tctccaagcg gtacgctttt ctgggcaggc tttttttgca ggaaatcgat
     1441 ctgtttttct ccagtgaaaa tgcgttgtcg accttttttg ccaagccgct ttggcttcgg
     1501 ttattcatat tgctggttat tgaaatgatg tggattacgc tggtgtcggt attggcaacg
     1561 cttgtggaat ggcggatttg gtttgaagcc tattttttac tctgctatgt accgtgctta
     1621 atttactatt ttttccattg tcgattcctc tggcataacg attttatgat ggcatgtgac
     1681 atgtatttcc gttgggggca ttttaataag tgaggaagtt acaggaagtg accagataac
     1741 gcatatatgt tctctactat cttgcgcatt ttgattgttg actgagtaac cagacagttg
     1801 atgtgcacaa tttcccctcg ccctaacaga cgtgggcggg ggcacccggt gtgtgcaata
     1861 tacgaaaaaa aagcccgtac tttcgtacgg gctcttcttt aaatatggcg gtgagggggg
     1921 gattgactcg cttcgctcgc cctgcgggca gcccgctcac tgcgttcacg gtctgtccaa
     1981 ctggctgtcg ccagttgtcg aaccccggtc ggggcttctc atccccccgg tgtgtgcaat
     2041 atacgaaaaa aaagcccg
//
LOCUS       DAZFVO020000328         2016 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFVO020000328 DAZFVO020000000
VERSION     DAZFVO020000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.330"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..655
                     /locus_tag="ACHKNB_RS25695"
                     /old_locus_tag="pgaptmp_005139"
     CDS             <1..655
                     /locus_tag="ACHKNB_RS25695"
                     /old_locus_tag="pgaptmp_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4198469.1"
                     /translation="LVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTH
                     SDENNKRTLISANTYADQKEIAINNRTDGLLMTERQARIAGDAETLATAEAYSDAGDS
                     LTLKQATAYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPY
                     VAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            complement(1581..1823)
                     /locus_tag="ACHKNB_RS25700"
                     /old_locus_tag="pgaptmp_005140"
     CDS             complement(1581..1823)
                     /locus_tag="ACHKNB_RS25700"
                     /old_locus_tag="pgaptmp_005140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198470.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT          631 a          489 c          412 g          484 t
ORIGIN      
        1 actggtcagt gcgaatactt atgctgacca gaaagaaacc gacatcaata accgtactga
       61 cggattactg acgaccgaac gccaggcacg caccgcaggc gatactgcaa ctctggctgc
      121 tgccagaacg cacagcgatg aaaacaacaa aagaacactg atcagtgcga atacctatgc
      181 tgaccagaaa gaaatcgcca tcaataaccg tactgacgga ttactgatga ccgaacgcca
      241 ggcacgcatc gcaggtgatg cagaaactct ggcaacagca gaggcataca gtgacgccgg
      301 ggattcactg acactgaaac aggcaacagc ctacagcgat atcagtaccc gccgggcact
      361 gtcagaggcc aatcgttata ccgacaacaa attcagaaac ctgagcaata aaattgaacg
      421 ggcagaaaaa agacttaatg caggcatcgc cggagtgaca gcaatatcct ccatccccta
      481 tgtggctgaa aattcttttt catacgggat cggcgtgggt aactatcaga acgctaatgc
      541 tgtggccgca ggcattcagt ataaaacatc acgtaatacc aatatacgcc tcaacatctc
      601 ctgggactca tctcataaca ccgcgattgg tgcaggggtt gccggcggct ggtaaataat
      661 cttgcgtaca tacggcatta tggtattccg taatcacatt aattcagtac gcaacacagc
      721 cccatccgga acagaccact ggactgagcg ggataatcaa ggaaaaaggg cgcgcagtgt
      781 ggtcagcctt cacaacaatc cggcggcaga tttcctgccg ccatgttgcc cgtaaacatt
      841 taagttcgca ggctaacatt attgccacaa gccccgtatt tccctggtga atcctcaaag
      901 cgagtccctc accatccaga caaaacacag tatcgtcgat taataatcac taaaagcgtt
      961 cgtcgtagca tcataccccc tgaatgaaca catccctgtt tgcaaggatt ctgttgttct
     1021 gatatcgacg agtagctgca actctgtatc cgcacttatt gcaatcagtg cagtctggcc
     1081 acgatgcgat tcgctgacct gagccttatt ctccaggcgg actttatgca atgctcccct
     1141 gaaatcttcc attattttac atgtacaggc atcatattaa atatggaaat aataacaaac
     1201 ggaaaaggga cacaatgaat atcaaagcac cagtataaac caggatgttt tgagcaattt
     1261 cttactctcc tgaatacaca ctgtaaatca ttagaaactg cactagccat gctaagtaca
     1321 ctctgctacg aactgatgag gaagtgcggt atcaattacc tggacagcct gatcaacgga
     1381 gaggtatatc tattatctat gtaattacag ggggactgat atcagcgaaa aagcaggtca
     1441 gcatattcac tgcccctggt ctgaactgaa acaacgtcag ccagaaataa tcctataatg
     1501 cctcatataa gtggataact tttcatccgt cgttgacatc atacacataa aacattgata
     1561 acagtcatga atacaccgaa ttatctttct gactcagcgt ttccagtcag tagcatttct
     1621 tccggtaaag gcatttccga aacgtcacgt tcgccattaa atacaaaaat actttctgcg
     1681 taatcattct gtaacaaaga attagttaac ggcagatttt ttgtttgcat accggtggcc
     1741 cactgaaaaa tcccggggaa acgacgggca ctgatgtgta cagaattgag gtggtacgca
     1801 gtaatatctc ttgccatcat cataagatgg cacatcatat cccgaacgca aacctggcac
     1861 atgatgaaca gacggaaggc tcagcaggac ttctggataa ttatacacga tagccagtta
     1921 ctgctgacct ggatttatta tcataccaca ttatttattt caaaaaataa attttgaatt
     1981 acttgttttt acttgtatat aaatatccaa cgatgg
//
LOCUS       DAZFVO020000329         1989 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFVO020000329 DAZFVO020000000
VERSION     DAZFVO020000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.331"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            62..>1989
                     /locus_tag="ACHKNB_RS25705"
                     /old_locus_tag="pgaptmp_005141"
     CDS             62..>1989
                     /locus_tag="ACHKNB_RS25705"
                     /old_locus_tag="pgaptmp_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HGU4198471.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVLKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKMKCRHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYIGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKP"
BASE COUNT          514 a          503 c          574 g          398 t
ORIGIN      
        1 cctgaagaaa tctggcccag tcgataccag gttaagagct atatgagaaa ggcgtcatga
       61 tatgtatgtc attgcaaaag aactgattgg cgcgcccgga atgcctgcta caacaaaagg
      121 tattcgccag gcattacaac gttacgtaca agggaaaagc tgttgttccc gtcgtcgctc
      181 aggctctaaa gcaactgaat acagcatcga ctgtttacct gaagtgacgc agcaggcatt
      241 acgtgaacgc tatgccctgc aactgatgac gcaaaaagcc gatgaatcac cggctccggt
      301 ggtgacaaag gccaggcgct cacctgccgt ggttgatgcg gtggaggcat atcgcggatc
      361 accccaactg atggtcgaac gcctcaatgc cctgactgaa aatcagcgcc aggtggctga
      421 tgcacgaatc gcgatcgtca gcgaagttct gaaagtcgcg caacaacctg gtttcagctg
      481 tgcgaaggct atccggttta tcgttgacaa cctggcacgt tcgcagctgg acgagcgcat
      541 tgtggcaatg gttgagacgg cgaacgccaa aaagggaaac agccgcgcgt tgagtgaaat
      601 cacgctgaaa cgctggattg cagcctttaa caaggcgcag aacgccgctg aacgcctgct
      661 tttgctggca ccgggtaaac gccaggaaat aaaagccgaa gatattaact ggctgcccga
      721 atttctggcg cagtatcgcc agtcaaacgg ccgaccaatg accgaggctt acgaggattt
      781 tgttgctgaa tggcagcacc ggcacgctga tgagccttat atgctcgata tcatgccgtc
      841 ttatgacacc attcgccgcg caatgaagaa actgccggaa gtggtgaaac aaaaaggccg
      901 ggtgacaggc agtgaatacc gtcagcttga gggattcacg cgccgcgact ggtccagaat
      961 gccggtgaat tatgtctgga ttggtgacgg tcacggcatg aagatgaaat gcaggcaccc
     1021 ggttcacggt cggccatttg caccggaagt gacctttgtt atcgacggtg gcacgcgctt
     1081 tgtggtgggc tggagccttg acctggctga aaatgttttc gccgtagccg gtgccataca
     1141 gcacggcatt cgccatcacg gcaaaccgtt tctgtattac tcggataacg gctccgggga
     1201 aaccgccgac atcctggata aggaggttgt ggggatcctg ccgcgactgg ggattaatca
     1261 cccaaccggg attgccggta acccgcaggg acggggcatt atcgaacggc ttaaccgcac
     1321 gttaccgatg cgcatagccc gtaaataccg cacctatatc gggaaaggtg cagatcgcga
     1381 gacgttacgc aaaaccaacc gcgatttacg ctcggcattt actgccctgc aacagggcaa
     1441 acggctgaac gcccggcagc agtcagcgat gcgtgattta ccgtcctggt ctgaactgat
     1501 tgatgccatt cgtgacggtg ttgagtggta caacaaccgg ccgcacgatg aattacccat
     1561 gaagccgaac gggaaacatt acagcccggc ggagttcaga aaaaaacgcc tggcggaaga
     1621 ggacactgaa attgaatggc tgtccgatgt tgagctgcgg gatatgttcc ggccgatggt
     1681 ggaacgccct gtaagacgct gtgaaatacg ctggctgaat aatatttact acgcgcccga
     1741 gctgcgtgat gagcatggcc gcaaggtgct tatcagctat gacattcatg atgccgaacg
     1801 aattaccgta cgtcgcctgg atggcagcgt gatttgcgag gcggtatggg acggtaataa
     1861 acgcgaagcc ttcccggtca gcgcggaata ctacaaacag cagcaacgcc ttaaaggcat
     1921 gcgtaaacgc gcagaggaaa aaatccgcga tgctgaggat gaagtggtta acgtgctgga
     1981 gcacaagcc
//
LOCUS       DAZFVO020000330         1905 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFVO020000330 DAZFVO020000000
VERSION     DAZFVO020000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.332"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..204
                     /locus_tag="ACHKNB_RS25710"
                     /old_locus_tag="pgaptmp_005142"
     CDS             <1..204
                     /locus_tag="ACHKNB_RS25710"
                     /old_locus_tag="pgaptmp_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HGU4198472.1"
                     /translation="ADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHDELHADTVAFEE
                     KYGSQLELIFRFIDRALAIGVLA"
     gene            218..970
                     /locus_tag="ACHKNB_RS25715"
                     /old_locus_tag="pgaptmp_005143"
     CDS             218..970
                     /locus_tag="ACHKNB_RS25715"
                     /old_locus_tag="pgaptmp_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HGU4198473.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAESKARCERCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEDQLRKVTR"
     gene            1119..1195
                     /locus_tag="ACHKNB_RS25720"
                     /old_locus_tag="pgaptmp_005144"
     tRNA            1119..1195
                     /locus_tag="ACHKNB_RS25720"
                     /old_locus_tag="pgaptmp_005144"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1153..1155,aa:Arg,seq:tcg)
     gene            1209..1285
                     /locus_tag="ACHKNB_RS25725"
                     /old_locus_tag="pgaptmp_005145"
     tRNA            1209..1285
                     /locus_tag="ACHKNB_RS25725"
                     /old_locus_tag="pgaptmp_005145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Arg,seq:tct)
     gene            1377..>1905
                     /locus_tag="ACHKNB_RS25730"
                     /old_locus_tag="pgaptmp_005146"
     CDS             1377..>1905
                     /locus_tag="ACHKNB_RS25730"
                     /old_locus_tag="pgaptmp_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4198474.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDA"
BASE COUNT          494 a          401 c          488 g          522 t
ORIGIN      
        1 gctgatgatc cccaccacct gataggtcac ggtcagggtg gaatgggaac aaaagcgcat
       61 gacctctttg tgttgccttt gtgtagaaag catcacgacg agctgcatgc ggataccgtg
      121 gcattcgaag agaagtatgg ctcccagctg gagctgatat ttcgttttat cgatcgtgcg
      181 ctggcaatag gcgtactggc gtaagtggag aacgagcatg aaccttgaag ccttaccaaa
      241 atattactcc ccaaaatctc caaaattgag cgatgacgca ccggcgacag gctcgggtgg
      301 tttaacgatt acagatgtga tggctgcgca ggggatggtg cagtcgaaag caccgcttgg
      361 gtttgcctta ttcctggcaa aagttggtgt tcaggatcct cagtttgcga ttgaaggtct
      421 gctcaattac gcgatggcac tggataaccc gacattgaat aaattgagtg aagaaacccg
      481 gttacagatc atcccttacc ttgtgaattt tgcctttgct gattattccc ggtctgcgga
      541 aagtaaggct cgctgtgagc gttgtgctgg tactggattt cataatgtat tgcgcgaagt
      601 ggtgaaacac tccagaagcg gggaatctgt tatcaaggaa gagtgggtga aggaactatg
      661 tcagcattgc catggtaagg gagaagtcag cacagcgtgc agagggtgta agggtaaagg
      721 tattgtcctg gatgaaaaaa gaacccggct tcatggcgcg cctgtttata agatttgtgg
      781 gcgttgcaat ggaaaccggt ttagccgttt accaaccaca ctggcgaggc atcgtgtcca
      841 gaagctgata ccagacctga cggattatca gtggtacaaa ggatatgcag atgtcattga
      901 taaactggtt acaaagtgct ggcaggaaga agcgtatgct gaagatcaat tgagaaaagt
      961 gacaagatag ataatttcgc cgaagatgtc gacatgatgc ttgcattttt caaaaaatat
     1021 ggataaagtt ttcccaacga tgggctttgt atgtctaccg ttgataagat ttaagagccc
     1081 gccgctgagc gggtttttgt tgcatctcct gttaaatacc gccattagct catcaggata
     1141 gagcgccagc cttcgaagct ggttgcgcgg ggttcgagtc ctcgatggcg gtccattatc
     1201 ggtacactgc gttgttagct cagccggaca gagcaattgc cttctaagca atcggtcact
     1261 ggttcgaatc cagtacaacg cgccataatt atttaccagg ctcgcttttg cgggcctttt
     1321 ttatatctgc gccgggtctg gtatcgtatt acttcagcca aaaggaatac ctgtatatga
     1381 agtgtatatt gttaaaatgg gtactgtgcc tgttactggg cttttcttcg gtatcctatt
     1441 cccgggaatt tacgatagac ttttcgactc aacaaagtta tgtctcttcg ttaaattgta
     1501 tacggacaga aatatcgacc ccacttgaac atatatctca ggggaccaca tcggtatctg
     1561 ttattaacca caccccaccg ggcagttatt ttgctgtgga tatacgaggg cttgatgtct
     1621 atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta tatgtggccg
     1681 gattcgttaa tacggcaaca aatactttct acagattttc agattttgca catatatcag
     1741 tgcccggtgt gacaactgtt tccatgacaa cggacagcag ttataccact ctgcaacgtg
     1801 tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt tcatcatatc
     1861 tggcgttaat ggagtttagt ggaaatgcca tgaccagaga tgcat
//
LOCUS       DAZFVO020000331         1853 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFVO020000331 DAZFVO020000000
VERSION     DAZFVO020000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.333"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..234
                     /locus_tag="ACHKNB_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /pseudo
     CDS             41..234
                     /locus_tag="ACHKNB_RS25735"
                     /old_locus_tag="pgaptmp_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            276..605
                     /locus_tag="ACHKNB_RS25740"
                     /old_locus_tag="pgaptmp_005148"
     CDS             276..605
                     /locus_tag="ACHKNB_RS25740"
                     /old_locus_tag="pgaptmp_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4198475.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            586..867
                     /locus_tag="ACHKNB_RS25745"
                     /old_locus_tag="pgaptmp_005149"
     CDS             586..867
                     /locus_tag="ACHKNB_RS25745"
                     /old_locus_tag="pgaptmp_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198476.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            1050..1334
                     /locus_tag="ACHKNB_RS25750"
                     /old_locus_tag="pgaptmp_005150"
     CDS             1050..1334
                     /locus_tag="ACHKNB_RS25750"
                     /old_locus_tag="pgaptmp_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HGU4198477.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            1334..1609
                     /locus_tag="ACHKNB_RS25755"
                     /old_locus_tag="pgaptmp_005151"
     CDS             1334..1609
                     /locus_tag="ACHKNB_RS25755"
                     /old_locus_tag="pgaptmp_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGU4198478.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
BASE COUNT          506 a          427 c          455 g          465 t
ORIGIN      
        1 cctcgtcggg accactccga ccgcgcacat aagctctatc atggctgaca gcgggtatgg
       61 ctttgcaggg tggggatggg taaggtgaaa tctatcaatc agtaccggct tacgccgggc
      121 ttcggcggtt ttactccggt atcacatgta accacagcgt gccgccttac atgcgctgac
      181 gcgcataaac aacaagttgc tacattagct tacaggttaa ttcaccttgc ataagattag
      241 catatagatt aatgtttatc gaaatgggag gtcatatgtt tagcgttgtt tatcacccgg
      301 aagccaggga agaagccaca gcattaccca taaaaatcag ggtgaagttt gaccgtctca
      361 tcagcaaact ggaatatgac gcccgattac tgcgggaacc ggatacaaag cccctggggg
      421 atggactttt tgagatccgc acgatgggga cggacatagc cagaggtatc tgggtatacc
      481 acaaaggcaa taccatcatc atgctccggg ttttcatcaa gaagtctcag aaaacgccag
      541 caaaagaaat cgatctcgcc aaaaagcggt tagcggaggt acttaatgaa actggtaagc
      601 cataaagaac tgcataacga gtggatgcag gacgacgaat atcgcgctgc atggcaggaa
      661 gaagaacgta aggagaaact cagggagttg cttgcagaat ggcgtaagca tgacaatttg
      721 acaaaagcgc aggttgctga acgcatgggg gttactcctc ctgttatttc ccgcctggaa
      781 aacaacatca ctaaagccag catcgacacc ctgactcgct atgcccatgc gtgcggtatc
      841 aaaaaacctg ttattacgct gtactgaact ctgctctatg gctgccagtt ctggagaaat
      901 cagtaactaa attttaatta gctacgttat ggtaaatctg cctgatgtgt cgatttctct
      961 gaaagtaatt cggcttctgt gggctgcgtt tgccttgtgc cacctgaatg taatcactat
     1021 gattaaatat gattaacaac taatcggata tacaaatgaa aaacaatacc gcacaggcaa
     1081 caaaagtaat taccgcgcat gtgccattac ctatggctga taaagtcgac cagatggccg
     1141 ccagactgga acgctcccgg ggctgggtta tcaaacaggc actttctgca tggcttgccc
     1201 aggaggagga gcgtaatcgc ctgacgctgg aagccctgga cgatgtgata tccggacaga
     1261 ttatcgacca tcaggctgta caggcctggg cagacagcct cagtactgac aatccgttac
     1321 cggttccacg ctgatggaac tgaagtggac cagtaaggcg ctttctgatt tggcgcggtt
     1381 atatgatttt ctggtgctgg ccagtaaacc tgccgccgcc agaatggtac agtccctgat
     1441 acaggcaccg gtcattctgt taactcatcc acgtatggga gaacagttgt ttcagtttga
     1501 acccagggag gtcagacgga tttttgctgg cgagtacgaa atccgttacg aacttaatgg
     1561 ccagactatt tatgtattgc gcctgtggca cacacgagaa aacaggtaga ttgcgtgtgg
     1621 ggacaactta acatagcctg cagatattaa ttcaggaaca ttattatcag gccagagatt
     1681 ttgcattcca ttaaataatc ttgacgtata ggaaattgaa aaactgagcg atacccctgt
     1741 ccgcgcctta tgccacgtga gcttttctgc ttttcccacc actccggtgt tttctctcta
     1801 tgtatggtcg gtttttatac cttaatgggc cgttttttat acttttcccg gat
//
LOCUS       DAZFVO020000332         1841 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFVO020000332 DAZFVO020000000
VERSION     DAZFVO020000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.334"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..485
                     /locus_tag="ACHKNB_RS25760"
                     /old_locus_tag="pgaptmp_005152"
     CDS             <1..485
                     /locus_tag="ACHKNB_RS25760"
                     /old_locus_tag="pgaptmp_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGU4198479.1"
                     /translation="YSAKTARVKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQKKS
                     EQEDKPRRREAAAIPQPDETNPEMPPPVVISPGIEYMEDGLPDPVKAMGRLLVENINT
                     DPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG
                     "
     gene            482..>1841
                     /locus_tag="ACHKNB_RS25765"
                     /old_locus_tag="pgaptmp_005153"
     CDS             482..>1841
                     /locus_tag="ACHKNB_RS25765"
                     /old_locus_tag="pgaptmp_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGU4198480.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQNGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSL"
BASE COUNT          404 a          444 c          611 g          382 t
ORIGIN      
        1 gttattcggc gaagacagcc agggttaaag gctcgcagct ggcaaaagat ccggaggtgc
       61 ttgcgtttat agcccgtaaa cagtgcgaga cggtggaggt ggatgaggtt cctgtttacc
      121 ggcagaaaaa atcagagcag gaggataaac cccgtcgccg tgaggcggct gcaataccac
      181 agccggacga aacaaatccg gagatgccac cgcccgtggt gatatctcct ggtattgagt
      241 atatggagga cggtcttccc gatccggtga aagcgatggg gcgtcttctg gtggagaaca
      301 ttaataccga ccccaggctg gcgctggatg cggcttataa gctggcgcag ttcacgcacc
      361 acaaaaaagg ggatgccggt aaaaaatcgg caaaaggtga cgcggcgaaa aaagcggcta
      421 accgttttgc ggtgccacca ccaccccgac tggtggtgaa taatgataat gagggcaacg
      481 gatgatacct gtgtggagca cggcctgccc ggactgggca gagcgcctga aaaaggggct
      541 gtcgattatt ccggctccga tttatccgga gcaggccgca catgccctgg cgatttttaa
      601 acaactgcgg attgtggatg caccgggcag cccgacgttc ggggagtcct gtgcaccgtg
      661 ggtgtttgac ctggtggcgg ccctgtttgg ctcctacgat gcgcagaacg gtgtacgcca
      721 tatcaaggaa gtttttatcc ttatcccgaa aaaaaacagc aagtccacgc tggctgccgg
      781 gatcatgatg acggcgctgt tactgaactg gcggcaggcg gcgggctaca ccattctggc
      841 cccgaccgtg gaggtggcgg ctaacgcctt taatcctgcg cgggatatgg tcagacgtga
      901 cgatgatctg gatgacctct gccaggtaca gacacatatc cggaccatca cccacagggt
      961 gacggacacc acccttaagg tggtggcagc cgatccgaat acggtgtccg gtatcaagtc
     1021 tgtggggaca ctgattgatg aactgtggct gtttggcaag cagtacaaag cggaggacat
     1081 gttacgtgaa gccatcggcg ggcttgcctc ccgcccggaa gggtttgtgg tgtatacgac
     1141 cacccagtcg aatgagccgc cagccggggt gttcagacag aaactgcagt acgcccggga
     1201 tgtccgtgac ggcaaaattc atgatccgca ctttctgccg gtgatatttg agcatccgcc
     1261 ggagatggtg gagcgcggtg agcacctgct gatggaaaac ctcgccatgg tcaatccgaa
     1321 tctcggttat tcggtggatg aggcttttct gtaccgggag taccgtaaag cccgggaggc
     1381 tggtgaggaa gcatttcgtg gcttcatgtc aaaacatgcc aatgtggaaa ttggtcttgc
     1441 cctgcgttct gaccgctgga cgggtgcgga tttctgggag cagcagggca ggcgcgtcag
     1501 cctggacgat atcctgcagc gcgctgatgt ggtgacggtg gggattgacg gcgggggcct
     1561 ggatgatctg ctgggaatgt acgtgattgg ccgtgacagg gaaacccgcg aatggctggg
     1621 ctggggccat gcctgggcgc atgaaaccgc ggtggtcaga cggaagagtg aggcatcccg
     1681 gtttcaggat tttgtggcct gtggagacat gacgattgtc cgtcgggtcg gggatgacac
     1741 ggcggaagtg gcggagtatg tgcgtcgtat tcatgaggct gagttactgg agcatatcgg
     1801 tattgacccg tcaggtgtgg ggcagattct ggattcactg g
//
LOCUS       DAZFVO020000333         1838 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFVO020000333 DAZFVO020000000
VERSION     DAZFVO020000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.335"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..1270
                     /locus_tag="ACHKNB_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /pseudo
     CDS             1..1270
                     /locus_tag="ACHKNB_RS25770"
                     /old_locus_tag="pgaptmp_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1561..>1838)
                     /locus_tag="ACHKNB_RS25775"
                     /old_locus_tag="pgaptmp_005155"
     CDS             complement(1561..>1838)
                     /locus_tag="ACHKNB_RS25775"
                     /old_locus_tag="pgaptmp_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198481.1"
                     /translation="IITTLIYFMGSLIVALPFSYSVVVSAKMMEKINHLTTWQYFGLS
                     SYFVVSGVAICFICLDKLSKIKIAERLIVSNRRLGDKCSGGTLAAES"
BASE COUNT          490 a          410 c          497 g          441 t
ORIGIN      
        1 acacacatca atggtggcac gcaaaccgtt cagaattatg gtaaggcaat caataccgat
       61 atcgtctctg gcctacaaca aattatggca aacgggacag cggaaggttc cattattaat
      121 gggtgttcac aggtagttaa tgagggcggt ctggctgaaa actcggtgct taatgacggc
      181 ggcacactcg atgtgcggga gaaaggcagc gcaacgggga tacagcagag tagccaggac
      241 gctttggttg caaccaccag ggcgacgcgg gtcacaggaa cacgcgcgga tggcgtcgcg
      301 ttcagcatcg agcagggtgc ggcgaacaat atcctgctgg caaatggcgg cgtgttaacc
      361 atggagtcag acacctcttc tgacaaaaca caggtcaata cgggcggacg ggagatcgtc
      421 aaaacaaaag ccactgcgac aggcacgacg ctcaccggcg gcgaacaaat cgtcgagggg
      481 gtggcgaatg agacaacgat taacgacggc ggaatacaaa cagtttcagc taacggagag
      541 gcaataaaaa caaagatcaa tgaaggcggt acgctgacag ttaacgataa tggcaaagcg
      601 acagatatcg tccagaacag cggtgccgct ctccagacga gcacggctaa cggtattgaa
      661 atcagcggta ctcaccagta cggcactttt tccatttccg gcaatttagc gaccaatatg
      721 ttgctggaaa atggcggtaa tttattggta ttagcaggta ccgaagctcg cgactccacg
      781 gttggcagcg gtggagccgc caacggcagt tatcgcagca acgggctggg cggtcacatt
      841 gaaaccggga tgcgatttac cgatggtaac tggaacctga cgccgtatgc atcgttaacg
      901 ggggttcacc gctgataacc ctgaatatca tttatccaat ggcatggaat cgaaatcagt
      961 cgatacccgc agtttatatc gtgaactggg tgcaacgctg agttacaaca tgcgtctggg
     1021 gaacggtatg gaagttgagc cgtggctgaa ggcggctgtg cgcaaagaat ttatcgatga
     1081 taaccgggtg aaagtgaata gtgacggtaa tttcgtcaat gatttgtcgg gcagacgtgg
     1141 aatataccag gcaggtatta aagcctcatt cagcagtacg ttaagcgggc atcttggggt
     1201 ggggtatagc catggtgccg gtgtggaatc cccgtggaac ggggtagctg gtgtgaactg
     1261 gtcgttctga ccatcaacga aaaagcccac atctgtgggc tttcatgtca ccaggagccg
     1321 cggctccttt gcgtatcctt ttatgtctct tcaccgtctg gtcggtgtcc tgctgagact
     1381 gctaacttcc tgtttttatt ggtgttgtcc ttataccgtc caatcatgat tggtggagct
     1441 ggcgggagtt gaacccgcgt ccgaaattcc tacatagcat ttcaatgtct gtaaaaacaa
     1501 tgatttgctt tgattttcag ttggttagat ttatgcggtg tctgtccgtt ttatgggttt
     1561 ttaactttct gccgccaaag tgccgccact gcatttgtcg cctaaacgcc tatttgaaac
     1621 gattaatctc tcggcgatct taatcttgga gagcttatct aggcagataa agcatattgc
     1681 tactcccgat accacaaaat agctgcttaa accaaagtat tgccaagtag ttagatggtt
     1741 tatcttctcc atcatcttag cacttactac aacagaataa ctgaaaggaa gtgctacgat
     1801 gagactgccc atgaagtaaa ttagagttgt tataattt
//
LOCUS       DAZFVO020000334         1801 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFVO020000334 DAZFVO020000000
VERSION     DAZFVO020000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.336"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..533
                     /locus_tag="ACHKNB_RS25780"
                     /old_locus_tag="pgaptmp_005156"
     CDS             <1..533
                     /locus_tag="ACHKNB_RS25780"
                     /old_locus_tag="pgaptmp_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HGU4198482.1"
                     /translation="ACVRATAIESTAQRCKKNQIYSPKIVEQSQITAFDKSTNVRRAT
                     AFAISVINDKATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNE
                     EVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKF
                     DDNEIITSAIDKLKRS"
     gene            561..1519
                     /locus_tag="ACHKNB_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /pseudo
     CDS             561..1519
                     /locus_tag="ACHKNB_RS25785"
                     /old_locus_tag="pgaptmp_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1531..>1801
                     /locus_tag="ACHKNB_RS25790"
                     /old_locus_tag="pgaptmp_005158"
     CDS             1531..>1801
                     /locus_tag="ACHKNB_RS25790"
                     /old_locus_tag="pgaptmp_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4198483.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNV"
BASE COUNT          565 a          334 c          423 g          479 t
ORIGIN      
        1 gcgcttgcgt tcgagctacg gcaatcgagt caacggctca gcgatgcaag aaaaaccaaa
       61 tttattcacc taaaatagta gaacaatctc aaattactgc ttttgataaa tcgactaatg
      121 tcagacgtgc tacagcattt gctatttctg ttatcaatga taaagcaaca attccactat
      181 tgattaatct gttaaaagat ccaaatggag atgtcagaaa ctgggccgca tttgcaataa
      241 atatcaataa atatgataat agtgatatta gggattgttt tgtggagatg cttcaggata
      301 aaaatgagga agtccgtatt gaagcaataa tcggactttc ctacagaaaa gataaaaggg
      361 ttttatctgt tttatgcgat gagttaaaaa aaaatactgt ttatgatgat atcattgaag
      421 ctgcgggtga attaggtgat aaaacgctac ttcctgtttt agatactatg ttgtacaagt
      481 ttgatgacaa tgaaattata acttccgcta ttgataagct gaagcgttca tagcgtgagt
      541 tgcctatgca cagtggggga ttcccgccgg cacggtgcag atgctcgacc ggctggaaag
      601 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      661 gaccgtggag cagatgaaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      721 cctgtaccac tgcgaccatc gcggcctgcc gctggcgctc atcagcacgg aaggggcaac
      781 agcgtggtgc gcagaatatg atgaatgggg caacctgctg agtgatgaga acccgcatca
      841 tctgcagcag ctcattcgcc tgccgggtca gcagtatgat gaggagtccg gcctgtatta
      901 caaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg atccgattgg
      961 gctgaagggg ggatggaatt tttatcagta tccgttgaat ccggtcataa atgtagatcc
     1021 gcaaggtttg gttgatataa atttataccc cgaaagtgat cttatccatt ctgtagctga
     1081 tgagattaat atcccaggcg ttttcacaat cggggggcat ggtaccccca catctattga
     1141 atccgcaacg cgcagtatca tgacagctaa agatctagca tatctaatta aatttgatgg
     1201 gaattataaa gatgggatga cagtttggtt attttcttgt aatacaggta aaggacaaaa
     1261 ctcatttgct agccaattag ctaaagagtt acatacaaat gtaataggac ctgacacgct
     1321 atggacgtgg tgggggcgag gaactaatgg taagttaaaa atggatacag tgctaacagc
     1381 accaacgaac cttaattcaa ataaggatct aatggctata acaacaaaag accttggtaa
     1441 ttggataaca tatgggccat ctgggcaccc catttctaat atgcaaggta cgccagaaaa
     1501 acccagtgat ataagatagg ttgtagatgt atgaaagcat gcttgttact atttttttat
     1561 ttttctctta ttggtcaatt gcatggtgct gatgtgaaaa taaaagaaaa cgaaagtgtg
     1621 atgggatcta cagcaatgac ctatgattta agtgaagaaa agctgatgaa actgaagtat
     1681 aaatcacaac atggtgattc agaggcatca tttcgactat atcaatatta ctgctttact
     1741 aaaaataata ttgataaaca attgcgattc ttggaaagat cagcatctca ggggaatgtt
     1801 a
//
LOCUS       DAZFVO020000335         1785 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFVO020000335 DAZFVO020000000
VERSION     DAZFVO020000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.337"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..456
                     /locus_tag="ACHKNB_RS25795"
                     /old_locus_tag="pgaptmp_005159"
     CDS             <1..456
                     /locus_tag="ACHKNB_RS25795"
                     /old_locus_tag="pgaptmp_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4198484.1"
                     /translation="RGEIHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFV
                     REDDWWRNGQNLYLDNLADNGFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELL
                     HHIPEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            453..1034
                     /locus_tag="ACHKNB_RS25800"
                     /old_locus_tag="pgaptmp_005160"
     CDS             453..1034
                     /locus_tag="ACHKNB_RS25800"
                     /old_locus_tag="pgaptmp_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4198485.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSFFTGGATLAAWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          451 a          395 c          542 g          397 t
ORIGIN      
        1 cggggtgaaa ttcaccgctt ccgctgtgtg ccgcacctga ccggacggcg ctttgaacac
       61 ggtgtgacgg actgttacac cctgttccgg gatgcatacc atctggcggg gataacgctg
      121 ccggattttg tgcgtgagga tgactggtgg cgcaacggtc agaacctgta cctggacaac
      181 ctggcggaca acggctttta ccgggtgtct ccgtcctgtg cacaggcagg cgatatcctg
      241 ctgtgctgct ttggcgcatc ggtgccgaat catgccgcca tttactgtgg caacggtgag
      301 ctgcttcacc atatacctga acaactgagt aaacgggaga ggtattctga aaaatggcaa
      361 cgacgaacgc attctgtctg gcgtcaccgc cactggtccg catctgcctt cacggggatt
      421 tacaacgatt tggtcgccgc atcagcctgt atgtgaacac ggcagcggag gccatccgtg
      481 ccctgtcgct gcaggtgccg ggattccgct gtcagatgaa cgaaggctgg tatcagatac
      541 gtattgccgg tgaggatacc gcaccggagg cggtgtacgc ccgtcttcac gaacaactgg
      601 gtgagggagc ggtcatccac attgtgccgc gactggccgg agccggggga aatggtgttt
      661 ttcaggtggt gctgggggtt gcagcgattg tcgggtcatt tttcactggt ggtgcaacac
      721 tggcggcatg gggcgcagcc ctgagtgccg gagggctgac tgccaccacg atgctgttct
      781 cactgggtgc cagcatgata ctgggtggtg tggctcagat gctggccccg aaggcaaaga
      841 cgccggagta taaaagtacg gataacggta aacagaacac gtatttttcg tcactggaca
      901 acatgattgc ccagggtaac ccgatgccgg tgccttacgg tgaaatgctg gttggttcac
      961 gacggatatc ccaggatatc agcacccgtg atgagggcgg agacgggaaa gtggtggtta
     1021 tcgggcgggg ataaaaataa aaaaaaatcc cgcagagtta gcggagctgc gggaaataac
     1081 gatgaagatt aacgttatgg agttattttt ttgggcagaa aaacattaac gcagcgaaat
     1141 tataagcgcc acatggattt tgcgaaaatg tgaagaaatt cagaaatttt ttgctgcaga
     1201 ggtgcaggga gcttgccggg tatctgtggc agaagacaga aaacatcatg ccggggttaa
     1261 gggataaaaa aatcccgcag agtccggaga ctgcgggaca gaaccgatga agattaacgt
     1321 tcaaggagtt ttgtttttat tgacccgaaa aaactgtaac gccccggaat gatatctgcc
     1381 acgggaacgt acagaaaatg tgaagaaatt cagacttttt ctgtcgcagg tttacagaac
     1441 ggatgaccgc cagggtacca gtggcgtaaa tgagcaggat gttgtggtgt ggctgaagca
     1501 aaaaaatctc ccgtaatatc gccggatact gacgggagac gaatagtcac cttaaggagg
     1561 tctgataatt attgctttta ttgagcaaaa aaactgtaac gctgcgtcat tatagtggct
     1621 acaggcaatt gccggaaatg tgaagaattt cagaaatttt attccgtcat gacacaggca
     1681 cccttcgggg tgcctgttgt tttctggcat aaacagattc agacatcaga caggagaggg
     1741 ggacagagtg ggtaaagggg gcggcaaggg gcacacaccg cgtga
//
LOCUS       DAZFVO020000336         1747 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFVO020000336 DAZFVO020000000
VERSION     DAZFVO020000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.338"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(239..1741)
                     /locus_tag="ACHKNB_RS25805"
                     /old_locus_tag="pgaptmp_005161"
     CDS             complement(239..1741)
                     /locus_tag="ACHKNB_RS25805"
                     /old_locus_tag="pgaptmp_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198486.1"
                     /translation="MKKFYQFRDEQRKELEQHDFYSLISSDCIALKDKLLFAPVMAHF
                     IMNFRDMNKWVIRFDNNDNEYKSVINGGTIEDETHSRLFLEDWRKLYIDDKLNWKASD
                     VIYWLFISREMECFRKFGIDFMRLCVDDGGEPILRYSHSESGETCGNIFFSKISPIAD
                     QVANHLGISLRYFGTFHLNLENGHVWKSEGVFENIELSPDSYKKMATLSKRMFDIFEG
                     IHDSFYNYLSSYVLNGSHPSFFESLPVGKNVAPIYPEFVIENKSHNDGRHIEHINNYL
                     EKISSHEFFKWLINTSIDPQLKLKSFIPLWIVDIMGYRDINKYVFTYEQPESESEKII
                     NDYALHLSEHSRLFYHDWKSLQLDDMLRWSASDTLEFIFLNADMDMHRENIVKFSLFG
                     LKHRDPVIRFWFMMILELSGKEFFSHVGDIALQVESKYNIYLPYLCGRHATENEHEAY
                     NNMYEHFMVKEISHEQSDLIIQITDMVMRSLLNNLDISYRYVVNNLLAAR"
BASE COUNT          577 a          333 c          261 g          576 t
ORIGIN      
        1 taagttgagt ccacgagatg attaaaaaaa ctgtcagttt tttaatggat gcagtagcgg
       61 cgatgcgcta cgtgaattct gaggtttttc aggaaggact gaacgcgaga attggcggga
      121 gggcatcaaa atattttttg ggcatgaatg gaaattcccc ctgttctggg cattaaacaa
      181 atcccactct gaaaagtggg attttaagga gcatcagagt cataccaatg ctgttcttct
      241 aacgagcagc caataaatta ttcactacat atcgatatga gatatccaaa ttattcaata
      301 atgaccgcat aaccatgtct gtaatttgta ttattagatc actttgttca tggctaattt
      361 cctttaccat aaaatgctca tacatattat tgtatgcttc atgctcattt tctgttgcat
      421 ggcgtccaca taaatatggg agataaatat tatatttact ttccacctgt aaagctatat
      481 ctccaacatg agagaaaaat tcttttccac ttaactccag tatcatcata aaccagaacc
      541 tgataacagg atctctgtgt tttaatccga acaaagaaaa cttaactata ttttctctat
      601 gcatatccat atctgcatta agaaaaataa actcaagagt atcactggca ctccaacgta
      661 acatatcatc aagttgaagt gacttccagt catgataaaa taaacggcta tgctctgaca
      721 ggtgtaatgc ataatcattt ataatctttt cactttctga ttcaggctgt tcatatgtaa
      781 aaacatattt attaatatct ctatacccca taatatcaac aatccaaaga ggtatgaaac
      841 ttttcaattt caattgaggg tctattgagg tgttaatcag ccatttaaag aactcatgac
      901 tcgatatttt ttccaggtaa ttgtttatat gttcaatatg tctaccatcg ttatggcttt
      961 tgttttctat cacaaactca gggtatatag gtgcaacatt tttccctaca ggtaatgatt
     1021 caaaaaatga cggatgactt ccattaagaa cataactgga caggtaatta taaaaagagt
     1081 catgaatccc ttcaaatatg tcaaacattc tctttgagag agtagccatt ttcttataag
     1141 aatctggtga caactctata ttttcaaaaa caccttctga cttccataca tgtccatttt
     1201 caagattaag gtgaaatgtt ccaaaataac ggagtgatat tcccaaatga ttggcaactt
     1261 gatcagcaat aggactaatt tttgaaaaga atatattacc gcaagtttct cctgactcgg
     1321 agtgagaata tcgaagtatt ggttctcctc catcatctac acaaagcctc ataaaatcaa
     1381 taccaaattt tcggaaacac tccatttctc gactaataaa caaccagtat ataacatcac
     1441 ttgctttcca gttaagtttg tcatctatat atagcttcct ccagtcttcc agaaacaatc
     1501 ttgaatgtgt ttcatcctcg attgttccac cgtttataac agatttatat tcattatcat
     1561 tgttatcaaa tctgataacc catttattca tgtctctgaa gttcattatg aaatgagcca
     1621 taacaggagc aaataatagt ttgtctttca acgctataca atctgaagaa atcaaactat
     1681 aaaaatcatg ttgttcaagc tcctttcgct gttcatccct aaattgataa aatttcttca
     1741 tagtgat
//
LOCUS       DAZFVO020000337         1745 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFVO020000337 DAZFVO020000000
VERSION     DAZFVO020000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.339"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(37..369)
                     /locus_tag="ACHKNB_RS25810"
                     /old_locus_tag="pgaptmp_005162"
     CDS             complement(37..369)
                     /locus_tag="ACHKNB_RS25810"
                     /old_locus_tag="pgaptmp_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198487.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHIGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELQGIHNG"
     gene            complement(366..917)
                     /locus_tag="ACHKNB_RS25815"
                     /old_locus_tag="pgaptmp_005163"
     CDS             complement(366..917)
                     /locus_tag="ACHKNB_RS25815"
                     /old_locus_tag="pgaptmp_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HGU4198488.1"
                     /translation="MMILQPMGRKGRAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSYWGVYNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLERDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAGDVVLRELTP"
     gene            complement(921..1435)
                     /locus_tag="ACHKNB_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /pseudo
     CDS             complement(921..1435)
                     /locus_tag="ACHKNB_RS25820"
                     /old_locus_tag="pgaptmp_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1435..>1745)
                     /locus_tag="ACHKNB_RS25825"
                     /old_locus_tag="pgaptmp_005165"
     CDS             complement(1435..>1745)
                     /locus_tag="ACHKNB_RS25825"
                     /old_locus_tag="pgaptmp_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198489.1"
                     /translation="AADDVQWCSFRKSLPWIEGHDIPSMAWEFAQGVLTCETVYVVAE
                     VDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRYLIVDRTAAPAAISAEVA
                     "
BASE COUNT          459 a          483 c          378 g          425 t
ORIGIN      
        1 gcctcatttc cacaggtggg acattcaaca atttgctcat ccattatgta tcccctgtaa
       61 ttccggtaag gccgaaatat gacaccagtg cgtcacctgt ccgtcctcat cttcaaggca
      121 ggctccggtt tcttcatcaa caaaaaaacc ttcatacccg atatggccga tgcgcagctc
      181 ctgtcggtga ccgtactgaa caatcagtac aacagcctcc atatcaggcg ggaaagcatc
      241 attcagtgaa tgccagggat aaccgcgcaa atccccgaaa taaaccccag tcagggttcc
      301 gccacagacc ggcatcccgg cggcacatac cagaggttcg tctgattcaa caaataaggt
      361 cgttatcatg gcgttaactc ccgtaataca acatccccgg cagtccggcg atggtgataa
      421 ataaaattca ccgtactctc ggataattca aatttctcgc ctatttcccg gaaggtaaga
      481 cgtcgtggtg attcagcatc ccgtaattca cgaataagat gaacatcatc gtcaggtatg
      541 cgggtaaaag gcagtaattc tccgtatttt ttcatgctta cgccaatata tcgcgcccga
      601 ttgtggacag aatcccggtc tcgctccaga tattctccta tttgcttacc ggtcattgtt
      661 ctggcatttt ttcggataaa tttatcttca tcatgtttaa atctgggacg tttgcatttg
      721 agcaactccg ggtaagtacc gcgtaataaa acaatccggt tatatactcc ccagtaactt
      781 ctttttactc tgacagcaat atccttaacc ggggtggatg gataaagcgc aatcagcagt
      841 gcatcttctt caggtgtcca tgcgcggaca tgagcgggtg cccgaccttt tcgtcccatg
      901 ggttgtaata tcatcataca tcaccccctg actcgctttt cagcagcata aaactcatca
      961 gacagaattt cggtcatttt ccgttttgcg gaatcatctt cgcactgaag gtaaataaca
     1021 ttgtcaacgc agaaccactt aacggggcca aacagtattg ccgagaagtc aatgcccagc
     1081 cagaacaaca gcgcatcggt tctggcatac gtgacgggcg aatattcacg ccacagactg
     1141 ttcagttcat cagaggcaac acgcagggtt ttaggtatac gtgatgtgcg cggcgtgcag
     1201 ctccagccat tcgacgcagt cggttttctc cataagtcgc gatgaaaagg atatttgtca
     1261 tccataaagc gcagcccctt aaaacaaaat ccactgatac cggatacaaa taccgggcgg
     1321 cacgcaacat tcagcacagc ctcaaggcgt tttgcctcat cttttaccgt ctggcagtcc
     1381 tgctggtatt ttgccacgca gccagcgcgg aagggtttga agttttaaag aacattacgc
     1441 cacctccgca gaaatcgcgg ccggtgccgc tgtccggtca acaatcaggt aacgcagaac
     1501 gcgcgaggtg atattccagc cgcttaaacc gcagataatg acgcccaggc ggatgtcgat
     1561 atacgccatc acaaactggg gcacgccttc tttcatggcc tgctcatcaa cttcagccac
     1621 cacataaacg gtttcgcagg tcagtacgcc ctgagcaaat tcccacgcca tcgacggaat
     1681 atcatgcccc tcaatccacg gcagggattt tctgaagctg caccactgaa catcatcagc
     1741 cgcac
//
LOCUS       DAZFVO020000338         1668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFVO020000338 DAZFVO020000000
VERSION     DAZFVO020000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.340"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..212
                     /locus_tag="ACHKNB_RS25830"
                     /old_locus_tag="pgaptmp_005166"
     CDS             <1..212
                     /locus_tag="ACHKNB_RS25830"
                     /old_locus_tag="pgaptmp_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HGU4198490.1"
                     /translation="KDKAAGVSAETAQQRIKEFHSEKFFHSLRSTTTFAGRKYTNSDM
                     PSPKKMKLMADTISAVYLDGYEGRQ"
     gene            610..>1668
                     /locus_tag="ACHKNB_RS25835"
                     /old_locus_tag="pgaptmp_005167"
     CDS             610..>1668
                     /locus_tag="ACHKNB_RS25835"
                     /old_locus_tag="pgaptmp_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGU4198491.1"
                     /translation="MTTLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVFLGTTLSISC"
BASE COUNT          548 a          327 c          286 g          507 t
ORIGIN      
        1 tgaaagacaa agcagccgga gtatcagcag aaacggcaca gcaacgtata aaagaattcc
       61 actcagaaaa attttttcac tcactccgga gcacaacaac ctttgctggc agaaaataca
      121 caaacagcga tatgccgtcg ccgaaaaaaa tgaaactaat ggcagacacc atttctgcgg
      181 tttatctcga tggatacgag ggcagacagt aaggggattt accataatcc cttaattgta
      241 cgtaccgctg aaacgctctc atacgcgttc tgacgcaaaa acgcaaaaag acaaggaaac
      301 cccaggctaa accgaaaaaa tgcgtcagaa tcgttttcag gtacttgcat aactattcac
      361 aaaagcgatg cgcgatcgat ttcgactatt ccgacggacg aacaggttaa aatgataagt
      421 ttcctccctc cggatggaag gttcagcacg gggattttta cccccttcca tcaacctgaa
      481 tatgcatctg ggagtaccca ttagtttcta ctcaaacact atcgtagtgc tatctcaaaa
      541 tataaaacat accgatggcg taagcatttt aaatatgata aataagaata tttttatcag
      601 ggattaatta tgacaacact atatatttac cttgttcttg ctgcatcatt atcggcatta
      661 tcaactgaca tgattgtgcc tgcgttacca gaactacaga aagcatttca tgctgactac
      721 tcattaattc aactaagtat tagtggattt cttattgtat ttgcatttag tcaactgata
      781 tcaggaacca taggtgatta tttcggaaaa gtacgcgtaa tgactatatg cttaattata
      841 tttcttattg gcagtattgt atgttttatg gccaatgatt taaatatgct tcttttgggt
      901 agaattctac aggcaatagg tgcaggtgcc ggacctgtaa tatcaaaagc cattgctaaa
      961 gagtcttttc caccactaaa gctaaaaaga gcactttctg acatatcatc aacaagtgcg
     1021 ataataccat taatagcacc tctggccgga gcattaatac ttgattatta taactggaat
     1081 gtaatattta ttgtaatggg tgcatttagc attctaacca tactcctgtc acctaaagag
     1141 actaataaac aggagaaaaa aacacatcaa gataatgtaa aatttgtgac taaagatttc
     1201 atctgcggga caatactcgt ttcactaatg ctaagctcac tattttgcta catatcaata
     1261 tcacctgcac tatttatgag tgattatgga ttaaatacat ttcactactc tgttgttttt
     1321 tccctgtcag ttttgttttt catcattgga aaccaattgt caaaaatcga aaaaatatca
     1381 aacccatggg tcttatttcc cctaaatgca atatcagtta caccttttct tatattaaat
     1441 gatgctttta taatatgtgt tattggagca atgattttca atttaactct tggcgcatac
     1501 tatccaatag ctaatttcat ctcattgcag ataaacggtt cacgaactgg tttagcagca
     1561 tcaattacag gctttactca aacagtaagc gcagggacaa tcagtttcct ttcagtaaaa
     1621 ataagcgata atggagtttt tttgggaacc actttaagta ttagttgt
//
LOCUS       DAZFVO020000339         1661 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFVO020000339 DAZFVO020000000
VERSION     DAZFVO020000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.341"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(113..1261)
                     /locus_tag="ACHKNB_RS25840"
                     /old_locus_tag="pgaptmp_005168"
     CDS             complement(113..1261)
                     /locus_tag="ACHKNB_RS25840"
                     /old_locus_tag="pgaptmp_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4198492.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCYELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(1265..1531)
                     /locus_tag="ACHKNB_RS25845"
                     /old_locus_tag="pgaptmp_005169"
     CDS             complement(1265..1531)
                     /locus_tag="ACHKNB_RS25845"
                     /old_locus_tag="pgaptmp_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HGU4198493.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(1563..>1661)
                     /locus_tag="ACHKNB_RS25850"
                     /old_locus_tag="pgaptmp_005170"
     CDS             complement(1563..>1661)
                     /locus_tag="ACHKNB_RS25850"
                     /old_locus_tag="pgaptmp_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HGU4198494.1"
                     /translation="MCKGVTKSDFTYGYDEDIKEFIKDKTYPYGNW"
BASE COUNT          508 a          423 c          312 g          418 t
ORIGIN      
        1 gcgtgtttct gcccccagaa atgctccata tcgggttatc agccaccaga gcgcagccac
       61 accaataaat acaatccccg gtcgtccaaa gcgaccagac agacaggcca gtttattcat
      121 tctggtttcc tgatgtaaca gtactgataa aatgttcttc tccggatgag aataaagtca
      181 ggagagaatc attaccaaga cagaccagat cggctgccag agcagtaagc agccacggag
      241 cagcaggtcc cgggataccg caccattttt ccagtaacat acgtccggac ggttcccatc
      301 cggcaccata ctggtttcca atagtcatca gcggtgcagc aatcttttcc cagtgataac
      361 agtcccccag tacacgagcg gtacgacagg cagctgtctg ggtttcaagg aaaagtgtga
      421 attcatcatt aaacttgttg atatccagcg acatcgggcg tagcagacgg gctggatgcg
      481 gcaggttata tttttgcgca acatcatcgc tggtcaacaa aagtgcagcc agtccgtcag
      541 agtatgcatt tcccccatta agctgtatta ccagtatcaa atccacagtc agcactggtt
      601 gtttcagacg ctcatcaacc caccccatgg aaaagtcggg agtaacctcg atattgtcag
      661 gaacgacgcg ttggggaaat aacgcttccc atgcagaaac aaaagcagat ctgatttcag
      721 aatcaggctg atcggttatc agcgtcacat ttacagggag agcggcaggt aactgacaaa
      781 atttatccgt tatctccctt agcaagacac gtaaagaggc ttcaacctta tgcccactgt
      841 cagaaagata atctattttc tttacaaggc cgtactgcag aggcaattca taacaaagag
      901 tagctaccgt tattttatca ggcaaataaa cacaactggc agtaataaca agataccttt
      961 cggcccagga ttcccactgt ttctgagcat actccccctc ttttttaaaa aacaaatgtt
     1021 catccatctg gttgctccac tgccagtgtc gcaggcaaaa caggaaaaac cagccccccg
     1081 gaggagtcaa tgcaagccac cagatactga aatcatttaa tgctttcaca tattctgatg
     1141 cccggataat aaacaacaga gcaccaacaa cagccataaa tgcccctgtc ataaaccaca
     1201 acatcattga tggtttatga ggtaattctg ttgataatga ttttgttctg ctccatccca
     1261 tggtttatcc tattgcacat tccggtgcac tggatataac cttacaccca cacagacatt
     1321 tacatccatc aatcacaaca gctcttccat cagatatcca ttcaggagag ccctccacaa
     1381 taggattggt accatgtccg gaaacagggc aactaaccat atctccaaca agagctactt
     1441 ttttaccatc gacaatcatg gttgaagatg ccgaaataac tttaccacca tgtgtggtat
     1501 tatcaccaag caatacaaaa cctttagaca taaaactctc aaaaataaat tttcactttg
     1561 agtcaccagt taccgtaagg ataagtttta tctttgataa attccttaat atcttcatca
     1621 tatccatatg tgaaatcact tttagtaact cccttacaca t
//
LOCUS       DAZFVO020000340         1648 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFVO020000340 DAZFVO020000000
VERSION     DAZFVO020000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.342"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1053
                     /locus_tag="ACHKNB_RS25855"
                     /old_locus_tag="pgaptmp_005171"
     CDS             <1..1053
                     /locus_tag="ACHKNB_RS25855"
                     /old_locus_tag="pgaptmp_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HGU4198495.1"
                     /translation="LMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYV
                     RLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGR
                     NYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQY
                     QGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRTDTQVNAGKVLPEVFA
                     SGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKAQNFEAVAQYQFDFGL
                     RPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVDYKINLLDKNDFTKAL
                     GVSTDDIVAVGLVYQF"
     gene            complement(1243..1626)
                     /gene="quuD"
                     /locus_tag="ACHKNB_RS25860"
                     /old_locus_tag="pgaptmp_005172"
     CDS             complement(1243..1626)
                     /gene="quuD"
                     /locus_tag="ACHKNB_RS25860"
                     /old_locus_tag="pgaptmp_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HGU4198496.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          465 a          386 c          334 g          463 t
ORIGIN      
        1 ctgatggcga tgtctgctca ggcagctgaa atttataata aagacagtaa caagctggat
       61 ctgtacggga aagttaatgc caagcactac ttttcctcta acgatgcaga tgatggtgat
      121 actacttatg ttcgtctggg cttcaaaggc gaaacccaaa tcaacgatca gctgactggt
      181 ttcggtcagt gggaatatga attcaaaggc aaccgcgctg aatctcaagg ttcctccaaa
      241 gacaaaaccc gtcttgcatt tgcaggcctg aaattcggtg actacggctc aatcgattac
      301 ggccgtaact acggtgtagc atacgacatc ggtgcgtgga ctgacgttct gccagaattc
      361 ggtggcgata cctggaccca aacagatgtg ttcatgactg gtcgcaccac tggtgttgca
      421 acctatcgta acaacgactt ctttggtctg gttgatggtc tgaactttgc tgctcagtac
      481 caaggcaaaa acgatcgtag cgatttcgat aactacaccg aaggtaacgg tgatggcttc
      541 ggtttctctg ctacctatga atacgaagga ttcggtatcg gtgcaactta tgcgaaatct
      601 gatcgtaccg acactcaagt taatgcaggg aaagttcttc ctgaagtatt tgcttccggt
      661 aaaaatgcag aagtttgggc cgcaggtctg aaatatgacg ctaacaacat ttacctggcc
      721 actacctatt ctgaaaccca gaatatgact gtatttgctg atcacttcgt tgctaataaa
      781 gctcaaaact tcgaagctgt tgcacaatat cagttcgatt tcggtctgcg tccgtccgtt
      841 gcttacctgc aatctaaagg taaggatctt ggagtatggg gcgatcagga cttagtcaaa
      901 tatgttgatg taggtgcaac ctattacttc aacaaaaata tgtctacttt cgttgattac
      961 aaaatcaacc tgcttgacaa aaatgacttc actaaagcac tcggtgtaag cactgatgac
     1021 atcgttgctg taggtctggt ttaccagttc taatctgatt acgaaaaaga tatgttgcgg
     1081 gaggcgttgc ctccccaaca tataagtggc tccctcaagc cacttccttt aggagcacaa
     1141 ccttgcttct aactatataa accttctgtt atatattacc ctttattttt gggggcgttg
     1201 caacgcccca tttttaataa ctttcagtaa acaattggca tattaattag agttattaac
     1261 aacgatatcc atctctaacc ggatatctaa tgccattaac atcccttcaa ttatgccctc
     1321 agccttctgt aaccttttcc cgatataacc atcagagcag caatgcttac ctgccagtga
     1381 catgaatgtc ataccgacta cataataatc tactaataaa tcgtgcaaat cgctgttgtt
     1441 ctttttcaga cgggccatgc acccgcaaat aatcatcgcg tcatcgtcac aacattgcgg
     1501 gcgagatttt acttttgaag gaattaatcc cttaaaaccg gcggcaatgg acgaccaggt
     1561 cacatcttca tgattattag ccgcccacgc tccccaacgt tcaagaacca tctgaatatc
     1621 acgcattaac tttctccaca aaatcagg
//
LOCUS       DAZFVO020000341         1640 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFVO020000341 DAZFVO020000000
VERSION     DAZFVO020000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.343"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..1322
                     /locus_tag="ACHKNB_RS25865"
                     /old_locus_tag="pgaptmp_005173"
     CDS             <1..1322
                     /locus_tag="ACHKNB_RS25865"
                     /old_locus_tag="pgaptmp_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4198497.1"
                     /translation="GASENSARWGVDKPLYKDLISRTKAALAKNPKNRLLAVVWMQGE
                     FDIDAKPTEHSALFLAMVEKFRTDLAEKAEQCIGGRTETVPWICGDTTYFWKKKNEPA
                     YQAIYGGYKNKTDKNIHFVPLMTDENGANVPTNNPAEDPDIESIGYYGSTWRNNAATW
                     TSADRASHFSAWARRGIVSDRLATAVLTHAGRTTVKADVPSSETDAPTPSPSEAETVT
                     TTTLLSYRASESEGKLTEQGWSAGGGKAEIVADEGATGGTAMKLSKETGKGSWHLEHD
                     AGTGAELLKNGGLISCRFKASSELVANQYVIALYWPVSSLPQGVTLTGEAGNNLLAAF
                     YIQTDAKDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQD
                     STPFTLTQSPVSAFAADKLHVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            1470..>1640
                     /locus_tag="ACHKNB_RS25870"
                     /old_locus_tag="pgaptmp_005174"
     CDS             1470..>1640
                     /locus_tag="ACHKNB_RS25870"
                     /old_locus_tag="pgaptmp_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4198498.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDP"
BASE COUNT          461 a          370 c          445 g          364 t
ORIGIN      
        1 cgggtgcttc agagaactca gcccgctggg gtgtggataa gccgttgtat aaggatttaa
       61 tcagccggac aaaagcggcg ctggcgaaga acccgaaaaa ccgcctgctt gccgtggtgt
      121 ggatgcaggg tgagtttgat attgatgcga agccgacgga gcattccgcg ctgtttctgg
      181 cgatggtgga aaaattccgc acagacctgg ctgaaaaagc agagcagtgt attggtggtc
      241 gaactgaaac tgtgccgtgg atttgtggcg ataccacgta tttctggaaa aagaaaaatg
      301 aaccggcata tcaggcaatc tatggcggct acaaaaataa aacagataaa aacattcatt
      361 tcgtcccatt aatgacagat gaaaatggcg cgaatgtacc caccaacaat ccggcagagg
      421 acccggatat tgaatccatt ggttattacg gctcaacgtg gcgtaacaat gctgccacct
      481 ggacatctgc ggaccgggcc agtcatttca gtgcctgggc gcgccgtggg attgtttccg
      541 accgtctggc cacggctgtt ctgactcacg cagggcgaac cacagtaaaa gccgatgttc
      601 catcttctga aactgatgcg ccaacgccgt caccttcaga agctgaaaca gtaaccacaa
      661 caacactgct gtcttaccgt gccagtgagt ctgaagggaa gctgaccgaa cagggctgga
      721 gtgcaggtgg aggtaaagcg gaaattgttg ccgatgaagg tgcaactggt ggaaccgcga
      781 tgaagctgag caaggagacg ggtaaaggat catggcatct ggagcatgat gccggtactg
      841 gcgctgaact gctgaaaaat ggcggcttaa tcagttgccg ctttaaggca tccagtgaac
      901 tggtggcaaa tcagtatgtc attgcacttt actggccggt ttcctctctg ccgcagggtg
      961 tcaccctgac aggggaagca gggaataatc tgctggcagc gttctacatc cagacagacg
     1021 caaaagacct gaatgtgatg taccacaatg caaaagtggc gacaaataac ctgaagctgg
     1081 gaacctttgg cgcatttgat aatgagtggc atacgctggc cttccgcttt gccgggaata
     1141 acagccttca ggtgacgccg gttattgatg gtcaggacag tacaccgttc acgctgacgc
     1201 agtcaccggt cagtgcattt gcggcggata aactgcatgt gacagacatt accaaaaatg
     1261 cgacttaccc ggtgctgatt gacagcattg tggtggaagt gaaaaaagcg gtaacagaat
     1321 aaaaaaaatc ccgccggtca tgatatggct ggcgggagct acctaaccca taaaggaaaa
     1381 taaaaatact accgaaggag tagttatcag tcacttcgat gtttttaccc gataacgaat
     1441 tatgtagtca atatcaaagg taaggttata tgacattcgt tcatacaatg ctgctttatt
     1501 tctgcgctgt ggtcagtgcg ttatatctgg tgagcggcgg ttataaggtc atcagaaatt
     1561 atattcgccg taaaattgat gatgcggcag ctgaaaaact cagtaaaaca gcacaggcac
     1621 cgtcatcccc aaacgacccg
//
LOCUS       DAZFVO020000342         1639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFVO020000342 DAZFVO020000000
VERSION     DAZFVO020000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.344"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            468..>1639
                     /locus_tag="ACHKNB_RS25875"
                     /old_locus_tag="pgaptmp_005175"
     CDS             468..>1639
                     /locus_tag="ACHKNB_RS25875"
                     /old_locus_tag="pgaptmp_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4198499.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSQGSGE
                     GTLSQSGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRP"
BASE COUNT          392 a          312 c          516 g          419 t
ORIGIN      
        1 ataaacacga agacggtgaa cacctttctg tatatgggac ttggtaaaaa cggtcttaag
       61 tacaggagtg cgaagcacct ggttggacgt gcgtagccgg aatgcatatt cgtgagtcgg
      121 gtaatgagag ttgaacggtg gcagtgagaa tggaacagaa aaaaatcaga tgcggaaggc
      181 ggaaatacac agcgggtgcg atgtgcttca tgctgtttgt atggctgggt ggtgtggtga
      241 cattctgtgt gctggccgct ctgataatga ggtgaaaacg gaagagagtt tacttcctta
      301 attcgaactt tctgataact gagaacttgt taaatgctcg tgggtttata tatttaaatc
      361 aattggttaa tgctgacttc atctttatca gtcaaagttt gttttagtat gatgattgtg
      421 cccactgttt attgttgtcc gggagaaaat acagggaatt ccagggaatg aacaaaatat
      481 actcactcag agttaatgac agaaaagaac tggttgctgt accggaaacg gtggggggca
      541 ggaaaaagtc gtccggaatt cccggtgcgg ggatgctttc ccgtcttctt ctggcctcag
      601 gagccgttgc cggcgttctg ttttcttctc ctgcgctggc atccgttgtc gggaatactc
      661 tgccatggca gacctacagg gattttgctg aaaacaaagg ggcgtttcat gccggggcca
      721 caaatattcc gctttatggt aagaatgggg ctgtcagtgg gcggctggac aaggctccga
      781 tgatggattt cagtgttgtg gaccagatac tgggggtggc aacactgatt tctccgcagt
      841 atgtggcggg ggttaagcat aacggcagct acaatacggt gcgttttggt tatgcggacg
      901 atacgacata tcgccttgtt gaccgtaatg agcactggcg tgattttcat actccccggc
      961 tgaacaaact ggtgacagaa gttgccccgg tgagcgtcac ggatgccgga accggcaagg
     1021 gggtatacca gaaccgaagt cgttatccgg tgttttatcg tatggggagt ggaactcagt
     1081 acaccggagc tgcttccggt gcgctgacca gaatagcgga tgcttacgtc tggaggactg
     1141 gcgggacggt gggaagtccg gtcatttctg actggagtct ggtgtcaaac ccgggaaatc
     1201 tttaccaggg tgttaatggt ccgctggcgt cttacgggac atcaggagac agtggctcac
     1261 cgctttttgc ctgggatgcg gtgaagaagc agtgggtatt tgttgctgta cttcatggtt
     1321 atgccggtga gaacgggaag acgaactggt ttacggtgat tcctgcaggg gatgtgaata
     1381 acacgataaa gcaggacaat tcaggcacag ttgtgccggc agttgccggt ggtgacattg
     1441 tgtggaagta cagtcaggga agcggagagg gaaccctttc tcagagcggg caggtatgga
     1501 agatgaacgg cttcagggga gggagtctga atgacgggaa agatatcacc tttgggggta
     1561 agggcacggt tgttctgaag gatgatgttg tccagggggc tggttcgctc acgtttaatg
     1621 gtgattatac ggtcagacc
//
LOCUS       DAZFVO020000343         1639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFVO020000343 DAZFVO020000000
VERSION     DAZFVO020000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.345"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            25..1347
                     /locus_tag="ACHKNB_RS25880"
                     /old_locus_tag="pgaptmp_005176"
     CDS             25..1347
                     /locus_tag="ACHKNB_RS25880"
                     /old_locus_tag="pgaptmp_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGU4198500.1"
                     /translation="MGLMASFERGMERFLVPVAVKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGVFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1375..>1639
                     /locus_tag="ACHKNB_RS25885"
                     /old_locus_tag="pgaptmp_005177"
     CDS             1375..>1639
                     /locus_tag="ACHKNB_RS25885"
                     /old_locus_tag="pgaptmp_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGU4198501.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQ"
BASE COUNT          420 a          339 c          368 g          512 t
ORIGIN      
        1 atccaaaaca acaggtataa aaaaatggga ttaatggcct cattcgaacg tggaatggaa
       61 cgttttcttg ttccagttgc tgtcaagtta aactcacaaa aacatgttgc agcggtgaga
      121 gatggattcg tttttacgtt tccaattatc atggcaagtt cattaattat attaattaac
      181 tttgccatat tatcgccaga cggttttatt gccggattac tgcatctgaa tagcattttc
      241 cccaaccttg aaaaagcaca agctattttt actccggtaa tgaatggttc tgtaaatatc
      301 atgtcaatta tgattgcttt cctggtcgcc aggaatatgg cgattagcta tgagcaagat
      361 gatcttttat gcggattaac ggcaatagga gcatttttta ttgtttatac gccatatcag
      421 atgatagatg ggcaagcatt cctgacgacc aaatatctcg gcgcgcaggg ggtgtttgtt
      481 gctgttatcg ttgcattgat caccagtgaa atattttgtc gcttagctcg aaacccaaaa
      541 atcaccataa cgatgccggc agctgtaccg cctgcggtag cgcgttcatt taaagtttta
      601 ttgccaatat tttttgtcat ggtgttcttt tccgcactta attattgcct gacactgata
      661 tccccggcag gactaaacga tcttatttac acattaatcc agacgccgct caaacatatg
      721 ggaacgaata tctttgcggt aattatcctg ggggctgtgg gtaatttcct gtgggtgctg
      781 gggatccacg gacctaatac cacatcggca attcgagaaa ccgttttttc tgaggctaat
      841 ctggagaatc tctcctgggc cgctcaacat ggcactacct ggggcgcgcc atatccgatt
      901 acctggactt ctattaatga tgcattcgcc aactgcggcg gttcaggtat gactttgggg
      961 ttattgttgg ctatttttat cgcttctaag cgtgcggaat accgtgatct ggcaaaaatg
     1021 tcatttatcc ccggtatttt caatatcaat gaaccgataa tgtttggcct tcctattgta
     1081 cttaacccca tcatgatggt gccgtttatt atggttccca ttgttaactg tgccattggt
     1141 tacttctttg tttcgatgga aattattcca ccggttgctt atgccgtgcc ctggactacg
     1201 cccggacctt taattgcttt cctcggaacc ggggggaact ggctggcttt actggttggt
     1261 tttttatgtt taggtgtggc gacaatgatc tatttacctt ttgttattgc cgccaacaaa
     1321 gtcaataaca tgacaactaa cggataattc catttaccca ggaaggagat tgtgatgttt
     1381 gcagatgaag aacttgttat ggagttactg gtcaacgcgg ggcaggcacg ttctgacgcg
     1441 atggaagcca tacgttgtgc cgggcaaaaa gactggcagg gcgcaactca gctaatggcc
     1501 agttctgaat ctgcctgtct tcaggcacat aagattcaga ccgcattaat tagtcaggat
     1561 gaaggctgtg gcaaaataaa agttaattta attctgattc atgcacagga tcatttgatg
     1621 aatgcaatat tatgccagg
//
LOCUS       DAZFVO020000344         1626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFVO020000344 DAZFVO020000000
VERSION     DAZFVO020000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.347"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            4..738
                     /gene="nanR"
                     /locus_tag="ACHKNB_RS25890"
                     /old_locus_tag="pgaptmp_005178"
     CDS             4..738
                     /gene="nanR"
                     /locus_tag="ACHKNB_RS25890"
                     /old_locus_tag="pgaptmp_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HGU4198502.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(774..>1626)
                     /locus_tag="ACHKNB_RS25895"
                     /old_locus_tag="pgaptmp_005179"
     CDS             complement(774..>1626)
                     /locus_tag="ACHKNB_RS25895"
                     /old_locus_tag="pgaptmp_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGU4198503.1"
                     /translation="SETISESKSLSGRIYQDPEHNGEFELDRVLTDIYAPQLIIFLPD
                     KPNGTGILIIPGGGYRAVMLDKEGTALAPEFLSQGYTLFVLSYRLPSPAEEENINKTF
                     IDAISAMLYIRKNADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSKEQEIF
                     AIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFLLH
                     CCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFMDW
                     MYSHGFK"
BASE COUNT          557 a          341 c          290 g          438 t
ORIGIN      
        1 tatatgtccg acaacaaaaa aaccgtgtta cctgggctaa caacgaccag aaagcccatt
       61 gttacaaaaa aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag
      121 gagataaggg aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt
      181 ggaagaccat ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat
      241 aacaagggag aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta
      301 tcaggcattg ttaaagattt tttatccaca gctgatggca tcaaggattt tgaaagacta
      361 cgcatctttt ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat
      421 attcacaggc ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg
      481 tatgctcaat atgatattga atttcatagt atacttgctg atatacctga caataaagta
      541 ttaaaaacaa tacactcagc attttctgaa tggctgataa tggccaaacc tctggaaaag
      601 aataaaatga catatgaaaa taacaaaaaa acccatcagc agcatattga aatacttacg
      661 gccataaaag agcatgattg tgataaagct gaacagctaa tgtataatca cctttataaa
      721 cgaattgaag catcatagtt actcgacgct tcaccaggta gtcaccataa atattattta
      781 aatccatgag aatacatcca gtccataaac agttttatcc atcctccaac aggaagatct
      841 gtggcccact gaacactaaa gccatgccct cctttctcat agagatgcat ttcagcagaa
      901 acgcctttct ctattaggcc acggtacatg agcagactgt tttcaattga cacaagatca
      961 tcatcacaac aatggagtaa aaacacagga ggaaaacatg atgtgacatt aagatccgca
     1021 ctatattgta ttatattttc tgaggtacta ttgtccccca gtaattgctt cctggagtct
     1081 ggatgtgtca catcatcttt cattgatatt acaggataaa ttaacccgac aaaatcggga
     1141 atagcgaaaa tctcttgttc tttactgtca gaagaacgtt tgaaatcaca aatctgattc
     1201 acaaacgttg aggcgagatg ccccccagca gaaaatccta atacaccaat acgatgacgc
     1261 tgtagatgcc atttatcagc attcttacga atataaagca ttgcagatat tgcatcaata
     1321 aaagttttgt ttatattttc ttcctcagct ggcgatggta atctgtaact aagaacaaat
     1381 aatgtatagc cctgagataa aaactctgga gcaagagctg taccttcctt atctaacata
     1441 acagcccggt agcccccccc gggaataatc aatattcctg ttccatttgg tttatctggt
     1501 aaaaaaataa ttaactgagg agcataaata tctgtcaata cacgatcaag ttcaaattcc
     1561 ccgttatgct caggatcctg ataaatacgg cctgaaagag atttactctc agatattgtc
     1621 tcactt
//
LOCUS       DAZFVO020000345         1611 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFVO020000345 DAZFVO020000000
VERSION     DAZFVO020000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.348"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..638
                     /gene="rfaL"
                     /locus_tag="ACHKNB_RS25900"
                     /old_locus_tag="pgaptmp_005180"
     CDS             <1..638
                     /gene="rfaL"
                     /locus_tag="ACHKNB_RS25900"
                     /old_locus_tag="pgaptmp_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HGU4198504.1"
                     /translation="FVVLSVIFIFLILGTLSRGAWLSVLVIGLIWILMFKQWKLLLVG
                     VMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGSALDLILENPVIGYGYGN
                     VAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGLVSLMMLYCAILKECIKN
                     GVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(674..>1611)
                     /locus_tag="ACHKNB_RS25905"
                     /old_locus_tag="pgaptmp_005181"
     CDS             complement(674..>1611)
                     /locus_tag="ACHKNB_RS25905"
                     /old_locus_tag="pgaptmp_005181"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4198505.1"
                     /translation="KRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYT
                     QIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGID
                     LETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVV
                     IGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQ
                     EPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANP
                     ELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          487 a          296 c          284 g          544 t
ORIGIN      
        1 cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta tccagagggg
       61 cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa caatggaagt
      121 tactattagt aggagtaatg gttgccatca ttgcattgtc ggttattttc acacataagg
      181 agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat cgctatgcaa
      241 atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt ggttatggtt
      301 acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat ccagaatgga
      361 cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt ggcactggtt
      421 tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag tgtataaaaa
      481 atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc ttactatctt
      541 ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat ttattaggcg
      601 tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag gctgcataat
      661 gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt tgagttacgc
      721 catcccaact gtagtgttca aacacaaagt cctgaccttg ctttgcaact gctgttaatt
      781 caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc ataggttctt
      841 ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca cgcgtactca
      901 ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat ggttcctgga
      961 actgggaagg aatgacgact aaatctgcca gtgggtaata acaatgcatc ttttcaggcg
     1021 gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc ctgacattgc
     1081 gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc acaactaatt
     1141 ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc aatatgcctt
     1201 tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggggag atattaagtt
     1261 cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt ccatttggta
     1321 caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag tataaactag
     1381 gcactatcat tttaacattt tgctcaagca attttggttc aaatgcatta tgcatatgta
     1441 taaccactct tgcctgagga gctcgcttac gaatttgagt gtatagcttc atgctattat
     1501 gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc aatattctct
     1561 gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta t
//
LOCUS       DAZFVO020000346         1603 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFVO020000346 DAZFVO020000000
VERSION     DAZFVO020000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.349"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(299..451)
                     /locus_tag="ACHKNB_RS25910"
                     /old_locus_tag="pgaptmp_005182"
     CDS             complement(299..451)
                     /locus_tag="ACHKNB_RS25910"
                     /old_locus_tag="pgaptmp_005182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198506.1"
                     /translation="MFEGLSDDSSPKEQQRDHRQHARGDTARRKSMTERSERIDEEAE
                     ENRKPH"
     gene            complement(896..1402)
                     /locus_tag="ACHKNB_RS25915"
                     /old_locus_tag="pgaptmp_005183"
     CDS             complement(896..1402)
                     /locus_tag="ACHKNB_RS25915"
                     /old_locus_tag="pgaptmp_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198507.1"
                     /translation="MAAQEVITDAQIRQRLLDLEEQNKKLRQELLDERRNTNFTQTYP
                     KGWERIRNLIQANPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVNF
                     LEENKCLVRIPIAGKLCAYALNPTEVWKGYNTSKPYAAFLTKTLVNSEGVIKRRLQAC
                     FGTSEPNE"
BASE COUNT          369 a          408 c          389 g          437 t
ORIGIN      
        1 aaaaaaacaa gcccttcagg gcgcgttagg gggtgtcggg gcgcagccct gacccagtca
       61 cgtagcgata gcggagtgta tactggttta ctcctctgca agcctcgtca gccgtgattt
      121 tgcccgattt cgcgctctcc acgttcaaca gctcgatcat gtttttgccg tccgttcacc
      181 gttaaaacgc ctcagaaatc gggacaggat gtgggaatgt cggaccgcgc acgcgcactc
      241 agacattccc cacctggtca gggcttcacc ccgacacccc gcatccgtgc gtaagtgctc
      301 aatgtggctt ccggttctct tccgcttcct cgtcgattcg ctcgctacgc tcggtcatcg
      361 acttgcggcg agcggtgtca cctctcgcat gttgtcggtg atcccgttgt tgttctttcg
      421 gggatgagtc gtcagatagc ccttcaaaca cgctcagaag ctctcagacg cacgaaacgg
      481 gttttagatg cccgccaggg catggaaggc gcgaaaaacg cttacagagg gtttcaggag
      541 gtcaggcgcg ccgatacccc taaccagggg ctttcaggtc gtcaggaaca gcgtcagccc
      601 cctgcaaagc ccatttgcac ggtacaggca gctattcggt tggtaggtgg tcgtagaccg
      661 tcggtatcac agcgataggg ggtctggggt tctccccaga cggtttccgg ttgcgctcaa
      721 ggcgcagccg aaaccgtggc ggctcggtcg ttctggagat cggggagcaa aaacacgcaa
      781 gggattttga ggcggcaaaa gagcaatgca ggatagacaa cccgatgtca aatgttaaca
      841 tttgaatcag attgtccaaa aacggggttt ttgcctgtgc ctgccgcagg ctctgttatt
      901 cattcggttc tgatgtgcca aaacatgctt gaagtcgacg ctttataacc ccctcgctgt
      961 taacaagtgt ttttgttaaa aacgcagcgt aaggtttgga tgtgttatag cccttccaaa
     1021 cctcagtagg attcaaagca taggcgcata attttcccgc gataggaatc ctaaccaagc
     1081 atttgttttc ctctaagaaa ttaacccaat tacgtatcgt tcttgttgtc acagataact
     1141 ggtctgctaa aaattgttgg tctgcaacga ctgcaccgca attaccgtca atgtgttctg
     1201 acaggaccga atacaatctt gctgctccag ggttagcttg tatgaggttt ctaattcgct
     1261 cccatccctt agggtaggtt tgagtgaaat tggtgtttct gcgctcatcc agaagctctt
     1321 gtcgcaattt tttattttgc tcttcgagat ccagaagtcg ttgtctaatt tgagcatcag
     1381 tgattacttc ttgagcagcc ataatacctc ccaattaatt tcctcataag cggaagaatg
     1441 attgcaaatt tatgttgttt tatttccaac gtcaatctat tcatttccta ttatgggaat
     1501 taaccggaaa gagcttgttt ccggttaacc ggaacgatcg tttccggtta cttttcgaaa
     1561 acccccataa aatcaaccat atagaacttt tctcctctta tct
//
LOCUS       DAZFVO020000347         1587 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFVO020000347 DAZFVO020000000
VERSION     DAZFVO020000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.354"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..420
                     /locus_tag="ACHKNB_RS25920"
                     /old_locus_tag="pgaptmp_005184"
     CDS             241..420
                     /locus_tag="ACHKNB_RS25920"
                     /old_locus_tag="pgaptmp_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4198508.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            461..706
                     /locus_tag="ACHKNB_RS25925"
                     /old_locus_tag="pgaptmp_005185"
     CDS             461..706
                     /locus_tag="ACHKNB_RS25925"
                     /old_locus_tag="pgaptmp_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4198509.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASYGGDPTPDSDMMM"
     gene            783..998
                     /locus_tag="ACHKNB_RS25930"
                     /old_locus_tag="pgaptmp_005186"
     CDS             783..998
                     /locus_tag="ACHKNB_RS25930"
                     /old_locus_tag="pgaptmp_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4198510.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1002..1337
                     /locus_tag="ACHKNB_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /pseudo
     CDS             1002..1337
                     /locus_tag="ACHKNB_RS25935"
                     /old_locus_tag="pgaptmp_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
BASE COUNT          441 a          330 c          458 g          358 t
ORIGIN      
        1 caactgacag gcatatcaaa ggcggcgaca tatacgctgc tgattgacag tgtgaaggtg
       61 gaagtgaaca acgcggacgt cgcggcatga taaaaaaggc cgccagcaat gaaggaaagg
      121 gatgaactgt gtccactaca tgaatgctgg cggctggtaa aacgaccatg atgacaccga
      181 agtctgcggt gtttttctac aaaaataagt aaatcatgtc aactatcacg gtgagaaacc
      241 atgacatttc tgaaccagtt aatgctgtac ttctgtacgg cggtctgtgt gctgtatctt
      301 ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca ggcagattga taaaagggcc
      361 gcagagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag
      421 ctgcaacttt cttaatcaca cctttcgacg agaaaatccc atgtcagaaa ttacatccct
      481 ggtcactgca gaggcagtga aggaagtcct gcgctctgaa gaagtcctga gcgcactgaa
      541 acagaaactc cgccagaacc ttgaggcgcg tcttgatgca gaagtggatg ccattctgga
      601 tgaactgctg ggcgcaccgg cagttccgga gccggaaggt atcgcgggtg aggggagtgc
      661 ttcatatggc ggtgacccca cacctgacag cgacatgatg atgtaagcat gcgcaaggga
      721 ccatcggtgt gtgccggtgg tctttatatt gttgtgagct tccggattgc gggagacggg
      781 gtatgtacca gatggaaaaa atcacaacag gtgtgtcata caccacgtca gcggtgggaa
      841 cgggctactg gttcctgcag ttgctggaca gggtttcccc gtctcagtgg gcggcaatag
      901 gtgtgctggg gagtctgctg tttggtctgc tgacatatct gacgaacctg tatttcaaaa
      961 tcagagagga ccggcgtaag gcggcgcggg gagagtagac gatgaataaa caatacgaac
     1021 tggttgttaa agggataaat aattacccgg ataagattac tgttactgtg gcgctggaaa
     1081 ctggtgggta tacgtcactg ttgttgccaa atgtggtgat tgatcttgat cgtgttgaag
     1141 gtgccccgct ggagttttac gaagctgagg cgaaaaagaa ggcgaagcag tttttcatgg
     1201 atattgctgc cgggttatgt gaagggaacg aaccgttgcc ggaaaagcgc cccataattt
     1261 tagaggcgca aaatgtgttg ataacctaca aaggaaagct accgggaaga attactgatt
     1321 ctctgaagat gccgccgtca acattgcggt cagaaaaaga tgatgttgaa tcacgtattg
     1381 aaaaactgga gcgctatatc gctgaattga aaaaaagcac cccaacaaaa aatgaggtgc
     1441 ttgcagcaga tgaaatgaaa gaagctattc ttgatcgcgc ggcgcatcta agctgcgctt
     1501 cacggttgaa agagcatctt cagcagcctg aaaaaaacgc cgcgatgagc agtttgcggc
     1561 gttttacgat tattgccgga aagtgat
//
LOCUS       DAZFVO020000348         1571 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFVO020000348 DAZFVO020000000
VERSION     DAZFVO020000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.355"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..599)
                     /locus_tag="ACHKNB_RS25940"
                     /old_locus_tag="pgaptmp_005188"
     CDS             complement(<1..599)
                     /locus_tag="ACHKNB_RS25940"
                     /old_locus_tag="pgaptmp_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198511.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFV"
     gene            complement(621..1160)
                     /gene="yhaB"
                     /locus_tag="ACHKNB_RS25945"
                     /old_locus_tag="pgaptmp_005189"
     CDS             complement(621..1160)
                     /gene="yhaB"
                     /locus_tag="ACHKNB_RS25945"
                     /old_locus_tag="pgaptmp_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HGU4198512.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1416..>1571)
                     /gene="tdcR"
                     /locus_tag="ACHKNB_RS25950"
                     /old_locus_tag="pgaptmp_005190"
     CDS             complement(1416..>1571)
                     /gene="tdcR"
                     /locus_tag="ACHKNB_RS25950"
                     /old_locus_tag="pgaptmp_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HGU4198513.1"
                     /translation="HIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSF
                     CECNRFP"
BASE COUNT          500 a          269 c          283 g          519 t
ORIGIN      
        1 ctaacgaaat ttacctgaaa catgttggca ttagtaaaat ctgtgtcatc caatttaaaa
       61 ggatttaaaa ttgcattggc catatgttta tctgtaaaat cataaagttc taaatcgata
      121 ttccgtaagt ccagagagga tagatcaact atacctccag tcccacaact aaaaactaag
      181 ttcaacctcc tgatttcatt gtaaatgtct ttcaatggga aattaacatt gatattgcct
      241 gtggatattt cctggttttt taacggttca ttaattgtaa gttcacaggt attgtctgat
      301 cgttgaataa ttagggcgtt ataaccaaaa aaatccatac gaagagacga atcattataa
      361 ataattacat tttgtaagta cgctaattct ttttgaaaat caccagtgat aatgcatttt
      421 ccacctggtt gtaacgcgtt aagtaaagtg accatttgaa tagaggtctg ttttagtttt
      481 tctatacctg gtgctagtgc ttcgaggtta ttaactatgg ggctttcagg aagatcattc
      541 atttttcgac ggactaaatc actactaata tcattaccaa ttgatgaaac agggaacatt
      601 attttatcct ttataaataa tcaataaaaa tcattattaa aatgatcggg tgaaaaaaag
      661 agaatttgcc aacaatctga agtaatgctg gttttattaa agtgcgcagg aggtgttaag
      721 atggcttctt ttcgggtgaa ttttgcacaa tacttagaaa tcgtctcctt aatacattcc
      781 agctcttttt tattgacggc tgagcaagta cattgttcac caaaaggtgc aaaagtgcct
      841 acatctaatg tttgtatgat ataatcgtca tgttctcctg tagaaatatc tatgatgaat
      901 ggactcatat tttccggttg gattcggatc atgacagaag gaacatcgcc ttgatagaaa
      961 ttcaatgcag taattgcaga taatactctc cgcagttcat tacttatact gtaaatttta
     1021 ttaacgtcat tggtggagac aatttctgca aactttcttt ttaccgtcca ataatcaata
     1081 tttctattgt gattgttaac cactgcgtgc attggcggga ttgaagggtg aaaaatatgt
     1141 gcaattggga agcctttcat tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc
     1201 ttattttcct cgcagggttc tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca
     1261 gtaaacgaag caacattctg tggcctacga cgatcaaaag ctctgtgaaa atttcccttg
     1321 ttgtctttgt ctgaccttat cgcggcgatc agcaattatt cgttttgtga tcctcaacac
     1381 aaaatgctat tcgtcgccta tgaggggaga agaatttatg gaaaacgatt gcattcacaa
     1441 aatgacgtcc tgtcagaaga gaaaaagttg acgttaatca attgttcgag atgatcaata
     1501 cctgtattcg aaattttaaa atattgataa aataaatgtt tgttctccaa caaatagcgt
     1561 gaaaaaatgt g
//
LOCUS       DAZFVO020000349         1570 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFVO020000349 DAZFVO020000000
VERSION     DAZFVO020000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.356"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..658)
                     /locus_tag="ACHKNB_RS25955"
                     /old_locus_tag="pgaptmp_005191"
     CDS             complement(<1..658)
                     /locus_tag="ACHKNB_RS25955"
                     /old_locus_tag="pgaptmp_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HGU4198514.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     N"
     gene            complement(822..917)
                     /gene="ymiC"
                     /locus_tag="ACHKNB_RS25960"
                     /old_locus_tag="pgaptmp_005192"
     CDS             complement(822..917)
                     /gene="ymiC"
                     /locus_tag="ACHKNB_RS25960"
                     /old_locus_tag="pgaptmp_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HGU4198515.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1031..1198)
                     /gene="yciX"
                     /locus_tag="ACHKNB_RS25965"
                     /old_locus_tag="pgaptmp_005193"
     CDS             complement(1031..1198)
                     /gene="yciX"
                     /locus_tag="ACHKNB_RS25965"
                     /old_locus_tag="pgaptmp_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HGU4198516.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1201..1341)
                     /locus_tag="ACHKNB_RS25970"
                     /old_locus_tag="pgaptmp_005194"
     CDS             complement(1201..1341)
                     /locus_tag="ACHKNB_RS25970"
                     /old_locus_tag="pgaptmp_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HGU4198517.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          431 a          371 c          360 g          408 t
ORIGIN      
        1 cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa gcaatccatt
       61 caccgtcctg caattcactc cacactgctt tgccgagata ttcgaggtta acctgatggc
      121 aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt ccccatttca
      181 ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct tcaaatgcct
      241 catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg accggtgaga
      301 gcgggttaac ctttgcagta tcgccgccga ggcgtttaac cgcttcgcgc attgccgcca
      361 gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc gggcggtagg
      421 caatttcacg gtcagcatgg gcatttttca gccatcctgc cagcgcgtgg atatcctctt
      481 cggtaaccga gttaccatcc tgccagcgca gcaggttttc gagcaaaact ttgagtgact
      541 tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg tagtagtggt
      601 aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt gacgacatag
      661 ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat attaaagata
      721 acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg agaaaccttg
      781 tggcaaagca aatgacaacc ccgccgcagc ggggtcaggg atcagtgagt aatgatccag
      841 aggagttcgg cccagaagag catcgacaga gaaaatgcgc ccatccagga ccaatatttc
      901 atatttgtgt tgatcatagg ctacaccctt aacggttatt actttgtgtt gagataactc
      961 aacctgttat tgctgcggtg tattaaaagc cctgtaattc attaaaaatg ggtaataaga
     1021 atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata aaggcttgct
     1081 gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt ttcgtttctg
     1141 tttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga ccgaccatag
     1201 ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag
     1261 aaaaaccgcc agccaggctt tacgttttaa ttcaggatcg cggcggggtt cctgatttcc
     1321 agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt tgaacgattg
     1381 gtgtaattaa ttatacgatg agacaaatcc taaagaaact ttagtcaaaa atccagcgaa
     1441 tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat atttgaaaac
     1501 tggaatttgc gaggcgggta attagacact actttacagg taatttaccc tataaatatg
     1561 agggataaaa
//
LOCUS       DAZFVO020000350         1564 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFVO020000350 DAZFVO020000000
VERSION     DAZFVO020000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.357"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..561
                     /locus_tag="ACHKNB_RS25975"
                     /old_locus_tag="pgaptmp_005195"
     CDS             <1..561
                     /locus_tag="ACHKNB_RS25975"
                     /old_locus_tag="pgaptmp_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198518.1"
                     /translation="VDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGEYIACYTK
                     PDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSSLPSTIRW
                     HSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVRLPAATGQ
                     NWQVMVSGFGQVERITLSTSMSEMPV"
     gene            558..>1564
                     /locus_tag="ACHKNB_RS25980"
                     /old_locus_tag="pgaptmp_005196"
     CDS             558..>1564
                     /locus_tag="ACHKNB_RS25980"
                     /old_locus_tag="pgaptmp_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4198519.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VS"
BASE COUNT          394 a          326 c          468 g          376 t
ORIGIN      
        1 gttgatgtaa acggtaatac agcactggca acggaaaaga ttatttcacc tgaacagtgg
       61 cagagtcagt ttaacccggc gtccattgtg gcttattcct ggcgtggtga gtacattgcc
      121 tgttacacga aaccggatgg taagcaggat gtgtttgtat tcagtccggt gaacatggat
      181 atccgttatc tcagtacacc gtttgactgc gcatgggttg atctcgcgaa agatatgatg
      241 cgcgtggtga caggagacaa aatgtcagtg cttgccggga gctctctgcc ctccacgata
      301 aggtggcatt caaaaatttt ttcattacct gaaagaacct ctttttcctg tatcagggtg
      361 aaatctccgg cgcctgagcg ggtggggatc accattatgg ctgatgatgt tcctgtgatt
      421 cattttgcgc cgggtacgtt taagggaagt gtggtgagac ttccggcagc aaccgggcaa
      481 aactggcagg tgatggtatc cggattcggg caggtggaac gaataaccct gagtacatcg
      541 atgtcggaga tgccggtatg accagaaaac cgtggcgtgc ggggaaggat ttatccacag
      601 ttgtggagaa catggaaatt ggcaccgggc agcgtggtga cggacgccac gcatttgtga
      661 cccgtgagga actggttggt cttaaactcg cccggcgtcg aacatcgggt ggtgcctcat
      721 atgcactgaa tccgggtatt gagattgaca gtactttaat gactgttgat tttcccacaa
      781 aaccgctgaa ttttaaggcg acaggaggat ttggctcggt tcttcttgaa tgggatatgc
      841 ctaattatcg cggacattca ctgactgaaa tctggcgggg tacggaggat gaccttgctg
      901 atgcagtgct ggttgccacg acgccggggc aggtttacgg cgatccggtt gaccctggct
      961 ggtcgggatt ttactggata cgttttgtta acgcggcagg agtgaaaggt ccatggaatg
     1021 ctgaaaaagg cactcaggca caaacacaga tcggcgtgaa ggccatcatt gaccagatcc
     1081 gcgatgaggc tgcaaagtcg ccggttgtgt ccgagctgcg taaagaaata aaaaacgcgc
     1141 aggggcaggc tgtaaaggat gctgcaatta agacaaccga agttgtgggg actctcaggg
     1201 aagaaacgac aagaacgatt ggtggtattg aaacccgcat tagcacactg gattcatcaa
     1261 ccagtgaatc gcttaatgag gtcgacaagc gcatcactaa actggataaa gaaggcggtg
     1321 aggcgtttct ggcaatgtgg tcaaaaaaag cgggagttga tggtatcact gcggggatcg
     1381 ggattgtcgc cggaaaagac agtgaaggca ggcctgtaag tcaggttgca atttctgcgt
     1441 cgcagttgtt tgtctttgac ccgaacaacc cggataacac cgcctatccg tttgcggtat
     1501 caggtggcaa ggttgtgatc ccgaaagcga tgatttatga cgcggtgatt gaaacactgg
     1561 tgtc
//
LOCUS       DAZFVO020000351         1563 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFVO020000351 DAZFVO020000000
VERSION     DAZFVO020000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.358"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..390
                     /locus_tag="ACHKNB_RS25985"
                     /old_locus_tag="pgaptmp_005197"
     CDS             <1..390
                     /locus_tag="ACHKNB_RS25985"
                     /old_locus_tag="pgaptmp_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HGU4198520.1"
                     /translation="KDLARSVASSLLSSSEMAFEQEKEPDGERWHDWSDPYRKWRTRK
                     GYMPGKILTLNGDLARRLTTDYGDTWALIGSNEPYAAIHQWGGLPGMPPGPAAIGARP
                     YMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            566..>1563
                     /locus_tag="ACHKNB_RS25990"
                     /old_locus_tag="pgaptmp_005198"
     CDS             566..>1563
                     /locus_tag="ACHKNB_RS25990"
                     /old_locus_tag="pgaptmp_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4198521.1"
                     /translation="MKTENHPKDANRPSGLAVLSASLTASGDGWCQLLPAGRVKARDG
                     RPEKPAEGWLINESACNRMKAGLSALNQPLLIDYDHHSMNAQKNGFKAIAAGWVKPEN
                     IEWREGQGIFIKPEWTPQAQKHIDDREYAYLSAYMHYFADTGEPYLLRMASLTNDPGI
                     TGMNPVAALSADDLYVVSPSQEQHPMNEQLRQLLTALGLTVADGDEFTPELGTAALSA
                     LTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVAL
                     SAQHGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTG"
BASE COUNT          378 a          428 c          433 g          324 t
ORIGIN      
        1 aaagacctgg cccgcagtgt ggcgtccagc cttctgtcat cctcagaaat ggcgtttgaa
       61 caggaaaaag agccggacgg cgaacgctgg catgactggt cagatcctta ccgcaagtgg
      121 cgtacccgta agggatacat gcccggcaaa atcctgacgc tgaacggcga tctggcccga
      181 cgcctgacca cggattatgg cgacacctgg gcgctgattg gatcaaatga gccttatgcg
      241 gccattcatc agtggggtgg cctgcccggt atgccaccgg gaccggcggc cattggtgca
      301 cgcccgtata tgggctttga tcaggtggca gagcaggaga tcatggacga aatcagaaaa
      361 cgctttaaaa aggccacaga aacgccttaa ttgtttaagg tatgtaaatg cattacctta
      421 cccccttcag gcgcgtgtcg tgattttcta acctgtattt aacgggcttt aaaatctgtg
      481 tgaagcgcct ttgcattttt ccctgaaaat acttcctgaa atcccgcaaa acccgccctg
      541 aaaattcccg gcttatgctg ccggtatgaa gacagaaaat cacccaaaag acgcaaatcg
      601 cccctctggt ttagccgtgc tttcagcttc gctgactgca tccggcgacg gatggtgtca
      661 gttactgcca gcaggcaggg taaaggcccg tgacggtcgc ccggaaaaac cggctgaagg
      721 ctggctgatt aacgaatccg cctgcaaccg gatgaaagcc gggctttccg ccctgaatca
      781 gccgttactg attgattatg accatcactc catgaatgcg cagaaaaacg gattcaaggc
      841 cattgccgca ggttgggtta agccggaaaa catcgaatgg cgtgagggtc agggaatttt
      901 cattaaacct gaatggacgc cgcaggccca aaaacatatt gatgaccgtg aatatgccta
      961 tctgtctgcg tatatgcatt atttcgctga caccggggaa ccctacctgt tgcgcatggc
     1021 ttccctgacg aatgatccag gtattacggg tatgaaccct gtcgcagcac tcagtgctga
     1081 cgatctctat gtagtttccc cttctcagga gcaacacccc atgaatgagc aattacgcca
     1141 gttgctgacg gcgctcggtc tgaccgtggc tgacggtgac gaatttacgc cggagctggg
     1201 cacggcggcg ctgtctgccc tcaccgggat caaaacccgt gcggatgcac acgacaacct
     1261 gaaaacacag gtcgccagtc tgtcggcaga gctggaaacc gcaaaaggca caccggctgg
     1321 cggcagtatt gatctgacga aatacgtgcc cgttgaaacg tataacgccc tgcgtaccga
     1381 atacgttgcg ctgtcggcgc agcacggcag caccacgctg gagcaggtgc tggacaaggc
     1441 tgaatccgaa gggcgcatct tcaaaagcga acgcggctat ctggaacaac tgggcgggca
     1501 gattggcgtg gcggcacttt ctgcacagct tgacgcccga cagccggttg cggccctgac
     1561 cgg
//
LOCUS       DAZFVO020000352         1548 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFVO020000352 DAZFVO020000000
VERSION     DAZFVO020000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.359"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(583..873)
                     /locus_tag="ACHKNB_RS25995"
                     /old_locus_tag="pgaptmp_005199"
     CDS             complement(583..873)
                     /locus_tag="ACHKNB_RS25995"
                     /old_locus_tag="pgaptmp_005199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198522.1"
                     /translation="MIVYPALLFCRLKILCVFLLPVLQKKPSRHGSGSALSATGNRLG
                     RTPDPHRCNTDGLRQPTNRMAACSVQMGFAGGWRGSWRPESPWLGVSARLTS"
     gene            complement(921..1397)
                     /locus_tag="ACHKNB_RS26000"
                     /old_locus_tag="pgaptmp_005200"
     CDS             complement(921..1397)
                     /locus_tag="ACHKNB_RS26000"
                     /old_locus_tag="pgaptmp_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGU4198523.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          355 a          411 c          385 g          397 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacc tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacgccga tacgattacc
      301 acctggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccgttgtt gttctttcgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcacaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaggcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggttgt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttagcac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccaac gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cgcgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat ttttccctct gaaaatccca tgcactaacg ttggttcaaa
     1441 tctaagccat catgacacat ctgtgcaaca ccttgttgcg tgttgtcgca tccaggatgc
     1501 gacaacgttg tgtcaccctg gatgcgacag gtaaataaaa aacacact
//
LOCUS       DAZFVO020000353         1535 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFVO020000353 DAZFVO020000000
VERSION     DAZFVO020000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.360"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(95..1501)
                     /gene="gndA"
                     /locus_tag="ACHKNB_RS26005"
                     /old_locus_tag="pgaptmp_005201"
     CDS             complement(95..1501)
                     /gene="gndA"
                     /locus_tag="ACHKNB_RS26005"
                     /old_locus_tag="pgaptmp_005201"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGU4198524.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDSEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQLAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          377 a          391 c          379 g          388 t
ORIGIN      
        1 attataaaaa aaggccataa catgccgctt tgtataataa aaaagcccgg agggtttctc
       61 cgggccttgc attgattaat tgatttaaat cagattaatc cagccattcg gtatggaaca
      121 caccttcttt atcaatgcgc ttataagtat gcgcaccgaa atagtcacgc tgtgcctgga
      181 tcaggttcgc aggcagaaca gcggcgcggt agctgtcgta ataggcaacc gcagcagcga
      241 aggtaggaac agggataccg ttctgtactg cataagcaac gacatcacgc agcgcctgct
      301 ggtagtcatc ggcaatttgc ttgaagtacg gagccagcag caggttagcg atctgcggat
      361 tttcggcata agcatcggtg attttctgca ggaactgcgc acggatgatg cagccagcac
      421 ggaaaatctt cgcgatttcg ccgtagttca gatcccagtt gtactcttca gacgccgcac
      481 gcagttgaga gaacccctga gcgtaagaaa cgattttgcc cagatacagt gcacggcgaa
      541 ctttttcgat gaactcagcc ttgtcgccag ctagctgcgc ttgtgggcca gagagaactt
      601 tagacgcagc aacgcgctga tctttcagag aagagatgta tcgtgcaaac acagactcgg
      661 taatcagcga cagcggttcg ccgagatcca gtgcgctctg gctggtccat ttgcccgtac
      721 ctttgtttgc cgcttcatcc aggatcacat caaccaagta gttaccgtct tcatcttttt
      781 tagtgaagat gtctttggta atgtcgatca ggtagctgct cagttcaccg ttattccact
      841 cggtaaaggt ctgcgccagt tcttcgttgg tgaggttcag accaccttta agcagagaat
      901 aggcttcagc aatcagctgc atatcgccgt actcaatacc gttgtgaacc atcttcacat
      961 agtgacctgc gccatcggca ccaatatagg taacgcacgg ttcgctgtct tcagccactg
     1021 cggcgatttt tgtcaggatc ggcgcaacca gttcataggc ttctttctgc ccaccaggca
     1081 taatggaagg acctttcagc gcgccttctt caccaccgga gacaccggta ccaatgaagt
     1141 taaagccttc tgcagaaagc tcacggttac gacggatggt gtcctggaag aaggtgttac
     1201 caccatcaat gatgatgtcg cctttatcga ggtatggctt gagagaatca atagcagcat
     1261 ccgtgcctgc acctgctttc accattaaca ggatgcgacg aggcgtttcc agagattcaa
     1321 caaactcttt caccgtatag taaggaacca gtttcttgcc tggattttcg gcaatcactt
     1381 cttccgtttt ctcacgggaa cggttgaaaa tagagacggt ataaccacgg ctttcgatgt
     1441 tgagcgcaag gttgcgcccc atcactgcca taccgacgac gccgatctgt tgctttgaca
     1501 ttgtttactc ctgtcagggg gtgttcaccg caggg
//
LOCUS       DAZFVO020000354         1501 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFVO020000354 DAZFVO020000000
VERSION     DAZFVO020000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.361"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(18..752)
                     /locus_tag="ACHKNB_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /pseudo
     CDS             complement(18..752)
                     /locus_tag="ACHKNB_RS26010"
                     /old_locus_tag="pgaptmp_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            complement(749..1080)
                     /gene="sctI"
                     /locus_tag="ACHKNB_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /pseudo
     CDS             complement(749..1080)
                     /gene="sctI"
                     /locus_tag="ACHKNB_RS26015"
                     /old_locus_tag="pgaptmp_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1100..1340)
                     /locus_tag="ACHKNB_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /pseudo
     CDS             complement(1100..1340)
                     /locus_tag="ACHKNB_RS26020"
                     /old_locus_tag="pgaptmp_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1354..>1501)
                     /locus_tag="ACHKNB_RS26025"
                     /old_locus_tag="pgaptmp_005205"
     CDS             complement(1354..>1501)
                     /locus_tag="ACHKNB_RS26025"
                     /old_locus_tag="pgaptmp_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4198525.1"
                     /translation="VDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNW
                     TFQE"
BASE COUNT          445 a          307 c          247 g          502 t
ORIGIN      
        1 ataacgctct catatgtcta cttaattttc aacctgacta aattagttag aatggcccta
       61 tacttccata acagccagca agtcgctacg gatattaatg caagtaagat agaaaccggc
      121 atagccttat cataagcaaa aacaggttcg ctaatttcat atgttggtgc ttgctcaata
      181 atgtctcttc gttttgacaa tacaacagaa atattttcat attgtacgct tgcagagcta
      241 ttaacaataa atctcttgat atcatttatt tttatttctg ggttgatatc tttttcatat
      301 actgcaagta cagaaatatg aattggtaaa gcagttttac cactatcgcc attatcaaca
      361 tcgtaactaa catgtactct cgaagaaata atgccatcca taattttgag agattgctct
      421 aaccgctgct caatagcaga atatagcctt gctttttccg ctcgtggaga tgaaacgagt
      481 gcatctgcag ggaacatctg cgatatttga atatcaggct tacccggtag attgtagatt
      541 tttagccaat ccaccgcaga agcaaaatcc gttggttcga caaatattga aaatcctgtt
      601 ttgccttaat ccttcttttc agcattaata ttatgtcttt gtaaaacagc aaggacatca
      661 ttagcttgct gttgatcaag atggttcaat aattcctgct gcttgcagcc gcacaacagc
      721 aggataaaca ataataatga tatatatttc atgagcgggt caacgtctca actgcagcga
      781 caatctttct tgctaatgtt ccaccaatga aacctcgaca ttataattaa gcacatgatc
      841 ctgcaagact aataattttt caggagttaa cccctcttta tcagacctta atgcttgtaa
      901 cgcattttgc tgccaatttt cagtttgtac agcatagttg gcaaaagcgg agatcatttt
      961 atcatccggg ttgtcaagat tgagaacccg gtcatttaaa tcaatcactt tcgcttgctg
     1021 tatttcatga gcagttgatt gctcagctct atcaataggt ggcatattgc ttactgacat
     1081 aataaaatcc ttatccagtt tagcggaagt tttgtatgat tgctgaatca agatctttat
     1141 acgcttttac aacgttagat tgcgcatttc gatataatgt atactcagct aatgcacttt
     1201 gatattcggc aagaaatttc gtgtcggagg gattggttgg ctaaatcctc caacgctttt
     1261 tcaacttgct ggttcagatc atcaacgccc tgatcaaatt gattgctgat atccataata
     1321 taaccattcc aatctgccat aagtcctcct caattattcc tgaaatgtcc agttgctacc
     1381 ttgagacttc tcgaatcggt tatctccgta gctaaatgaa ctattatttg tgtcctgatt
     1441 tttcaaattc acattaaatc ttacatattg tttaccccat tgtttataat attcgtcaac
     1501 a
//
LOCUS       DAZFVO020000355         1493 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFVO020000355 DAZFVO020000000
VERSION     DAZFVO020000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.362"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(62..289)
                     /gene="mchB"
                     /locus_tag="ACHKNB_RS26030"
                     /old_locus_tag="pgaptmp_005206"
     CDS             complement(62..289)
                     /gene="mchB"
                     /locus_tag="ACHKNB_RS26030"
                     /old_locus_tag="pgaptmp_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HGU4198526.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(306..515)
                     /gene="mchI"
                     /locus_tag="ACHKNB_RS26035"
                     /old_locus_tag="pgaptmp_005207"
     CDS             complement(306..515)
                     /gene="mchI"
                     /locus_tag="ACHKNB_RS26035"
                     /old_locus_tag="pgaptmp_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HGU4198527.1"
                     /translation="MSYKKLYQLTAIFSLPLTILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRKILVWSGWKNGS"
     gene            928..1173
                     /locus_tag="ACHKNB_RS26040"
                     /old_locus_tag="pgaptmp_005208"
     CDS             928..1173
                     /locus_tag="ACHKNB_RS26040"
                     /old_locus_tag="pgaptmp_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198528.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          515 a          298 c          272 g          408 t
ORIGIN      
        1 gaaagggata tattttacag gcactaccga aaaagtgccc gtaaaataaa taaaattata
       61 attagctacc gccaccagca gaagaactgg cactaccact tcccacggtc gagccaattg
      121 ctgttgtcaa accggcagat acggctccaa ctacaacccc aagtggacca ccaggtattc
      181 cggcgagagc tccaacaata gctgcagcac ctgcggcatt agctgaagtc gctggcgcac
      241 ctcccgcccc ggaaatatat cttaactgtg attctgttat ttctcgcata taaactccat
      301 catatttaac ttccgttttt ccagcctgac cagaccagaa tctttcgaat cccatgaatc
      361 agctcaataa aaccaagaaa tattataaag cttagaaaaa cgtcaacata agaattccct
      421 tcgccaacaa tccgaaggga tgaaagtgaa accaataaga tagtaagagg taaactgaat
      481 atagccgtca attggtacag ttttttataa ctcattaata cctacctgtt gctttatttt
      541 cattatgaaa agttaccaaa tcttagcgct tcagtcaggg aagtcaggtc atcgataaag
      601 aatattccag tagagtggcg ctcatgatca aagacaacag caaagttgat gacagtgctg
      661 accgactgtc atttacagta accctgtttg tagcaaattc catcttacct gacctcaccc
      721 agacaaagac aaaagagaat gattgttatt tacttttcaa aaaaaagtgt catattgtcc
      781 atataaagtc aagaggtatt ttacgttatg taactgatgc aattgttagt caggatctca
      841 tggttgtcgc tcatgtataa cctgagactt gaacgtacac taatagcact gacggtcata
      901 catcgggaaa tgtgaggagg cataagaatg aattgtgata ataatcacag aaatgaagaa
      961 ttcattgtta cctttgataa aggcaacaag caagacaatt caagacgaaa acacgataat
     1021 tttcctatag aggtagaatc ctccgtagag caggagacac actgtatcac aaataataag
     1081 tcggcttccg gtatagtaac acatgactat gatgccgatt atatttgtgg ttgtggtgaa
     1141 attatgtgtc ctggttgcgg tcatgaccta taacaaacat ttttgcaaac tgatacctga
     1201 tcaggtacaa gcctgttgat tattatgctg aatattccat gttataaaac catcgatata
     1261 aacattaaca agaaaaaaca tatattgttt atctgaacat ttatcgatgg aaaaccaaag
     1321 gggaaagtat gtatcttacg aaaaagatta taataagtat gatgtttata ttaccatctg
     1381 ctgcattttc atcagatcca cctccccttc aacaatcgtt agaaaaaaca acctattttt
     1441 ctataggtat gaatgggttt ataggctatc agagcgaagg ggaaaaatta tac
//
LOCUS       DAZFVO020000356         1486 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFVO020000356 DAZFVO020000000
VERSION     DAZFVO020000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.363"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            5..637
                     /locus_tag="ACHKNB_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /pseudo
     CDS             5..637
                     /locus_tag="ACHKNB_RS26045"
                     /old_locus_tag="pgaptmp_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(656..1158)
                     /locus_tag="ACHKNB_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /pseudo
     CDS             complement(656..1158)
                     /locus_tag="ACHKNB_RS26050"
                     /old_locus_tag="pgaptmp_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(1241..1459)
                     /gene="ygeI"
                     /locus_tag="ACHKNB_RS26055"
                     /old_locus_tag="pgaptmp_005211"
     CDS             complement(1241..1459)
                     /gene="ygeI"
                     /locus_tag="ACHKNB_RS26055"
                     /old_locus_tag="pgaptmp_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HGU4198529.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
BASE COUNT          465 a          228 c          281 g          512 t
ORIGIN      
        1 atatatggga aaaattaaaa ttgtagtttc agatcagcag ccgtttatga ttgatgggat
       61 aattggattt ctcggacatt atcctgattt atataaggtt gttgggggct ataaagatct
      121 gaagaaagct atagcggagt gtaacaaatc tacagcctaa atatttattt taggagaatt
      181 tgcaggtgga atgatggggt ccgaactcgt aaaatgggtt aaatcgcata agatagatgc
      241 tcatattatt acatttgtag caaagatgcc gtatattgat tcaataaaat tgcttgaagc
      301 aggtgcgaaa ggatgcgtat ggaaaaccag tcacccggcg aaactaaatc gtgctattga
      361 ttcgattagt aatggttaca cttattttga tagtgtacat atggattgtg aaaaaatatc
      421 ttccaggtat tcctctgata atcaacttac aaatcgtgag tcagaaatat tacaactcat
      481 agctgatggt aaaacaaaca aagaaatcgc taatttcttg cagttaagca gaaaaacagt
      541 cgaaactcat agactcaata tcatgaagaa attagatgtt cacagtggta tcgagttgat
      601 caaaacagct ttacgtatgg gtgtttgcac tatataaaaa aaagaggctt taggctcatt
      661 ttgctgaagc cttacgattg tctagttcat taagcctggt atatacaata tatattttgg
      721 ggcatattgc tgtctctttt tttggttatc cattgcaaaa cccgcattat aagtccccag
      781 gcattgccac gttactccac aacgtgaaaa atggttatat aaaatctcgg ttcctatttt
      841 aatgttcaga caagggttat ctaacaaatc tcgttcatct tttattattc cacgtttttt
      901 taacagggga atatgcatct ggtttatctg cattaaccaa taatctaaac tctttatatt
      961 gttattctta tctctgttct tgcctatgct atcctttttt agattgcttt caacaagagc
     1021 gatagcttta attaaatttg gttcgattct gaacattgtt cctgcttcat tagagcaaga
     1081 gttgctggcg aaagacattt ttgtaataaa tatacaaaac gtgaagatgg ttaagaattt
     1141 aacttttggt gagcgcatat tttttcatgt gtgaggattg aatgaaaggg acgagatact
     1201 attataaaag tatctcgtta tgatttattt ggtcaatcgt ctaccattca attttatagt
     1261 aaggtgagat aggattatct tctgaataca tatcattttg aaaaggcgcc atccatgttg
     1321 aaactataaa tgcttgtatt tgatctgtat cagtagcact ggtgttgatg tgtttatcta
     1381 atgatacgac ttcatcaccc gttgcattcg ctatatttga actttgagat aaattattga
     1441 taccgattgg attagtcata aagctccctt ttttaattgt ccaggc
//
LOCUS       DAZFVO020000357         1472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFVO020000357 DAZFVO020000000
VERSION     DAZFVO020000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.364"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..>1472
                     /locus_tag="ACHKNB_RS26060"
                     /old_locus_tag="pgaptmp_005212"
     CDS             41..>1472
                     /locus_tag="ACHKNB_RS26060"
                     /old_locus_tag="pgaptmp_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HGU4198530.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYL"
BASE COUNT          458 a          212 c          393 g          409 t
ORIGIN      
        1 ttgtttttaa tttattgttt taattatcaa agaggaattt atggctaaag aactaagtgg
       61 atatggccca actgctggtg agtctatggg gggaacaggg gcgaatctga atcagcaagg
      121 tggaaataat aatagtaata gtggggttca ctggggtggt ggttcaggct ctggtaatgg
      181 aggtggtgaa catggaagtc agacggggtg gggatggagt aaaaccaata atccggatgt
      241 gccgccttat gttgatgata atgggcaggt gcgcattacc attacaaatg gtctggtaaa
      301 aacgcctgta tatggagttc ctgaggctgg cggaaatagt gatgttcagg ggggctatat
      361 tcctgaaaat cctaatgatg aagttgctcg taagtgggat aaaaacaact taccgcgaga
      421 gattgatgtg agtatcgatg gttttaaata tcgtgtaacg cttaatgata atggtagagc
      481 tatcggtatt cttcgaacag gtgtccgtcc ttacgtaggg agtgaaaaag ctaaggcggg
      541 tattatggaa aaaatcaatc ataaaacacc tgaggagata tatgaggcgt taggttttaa
      601 taaagatgaa tctcagcggc aggaaaaagc aaaacaacag gctgaagatg cgtgggatcg
      661 tttgcctcct aatgtgagaa aatttgatgt tgatgttgaa cagttccact atttagtggt
      721 gcttgatgat tatggtaatg ttttatctgt aactagaaca gctgttcgtc cttatgtagg
      781 gagtgaaaaa gctaaggctg gtattatgga taaagtcgac cataagaccc cggaagagat
      841 atacgaggca ttaggattta ataatgaaga gcctcaacga cagaatcaag ctaaaaaagc
      901 tgcttatgat gtattttatt ctttcagtat gaaccgtgac cggattcaga gtgatgtgct
      961 aaataaagca gctgaactta tatctgatat gggtaacaag atcggtgatt atcttggtga
     1021 atcttataag tcgttagcaa gagaaattgc tgatgatatc aaaaatttcc aggggaaaac
     1081 catccgtagc tacgatgatg caatggagtc attaaataaa attttgtcta atcctagttt
     1141 taaagttaac agagcagacc gtgatgcact ggtgaacgcc tggcgaagtc gtgatgctca
     1201 ggatatagca aataaactgg ggaatatcag taaggctttc aaagctgccg atgtaattat
     1261 gaagattgag aaggtcaagg agaaaagcat cgagggatat gaaactggaa actgggggcc
     1321 gctgatgttg gaggttgagt cttgggttct cagtggtatg gccgcaagcg ttgccatgac
     1381 attgcttagt gctataattg gatcattcac actaccggcg ttagcattaa ctgcaataaa
     1441 tattgcaggt attatgggaa ttagttacct tg
//
LOCUS       DAZFVO020000358         1407 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFVO020000358 DAZFVO020000000
VERSION     DAZFVO020000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.365"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..189
                     /locus_tag="ACHKNB_RS26065"
                     /old_locus_tag="pgaptmp_005213"
     CDS             <1..189
                     /locus_tag="ACHKNB_RS26065"
                     /old_locus_tag="pgaptmp_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4198531.1"
                     /translation="EADEEPEDDVLMQKAAGLAGGVRFGPDGNEVIPASPDVADMTED
                     DVMLMTVSEGIAGGVRYG"
     gene            182..>1407
                     /locus_tag="ACHKNB_RS26070"
                     /old_locus_tag="pgaptmp_005214"
     CDS             182..>1407
                     /locus_tag="ACHKNB_RS26070"
                     /old_locus_tag="pgaptmp_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4198532.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQ"
BASE COUNT          316 a          348 c          486 g          257 t
ORIGIN      
        1 gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg gcttgccgga
       61 ggcgttcgtt ttggcccgga cgggaatgaa gttatccccg cttccccgga tgtggcggac
      121 atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg aggagtccgg
      181 tatggctgaa ccggtaggcg atctggtcgt tgatttaagt ctggatgcgg ccagatttga
      241 cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaactgatg cgaaaaaaac
      301 agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga aagcggggat
      361 ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca ccgacgtggc
      421 cacgcagctt gcaggcgggc aaagcccgtg gctgatcctg ctgcaacagg gtggtcaggt
      481 taaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggtg cgatcaccct
      541 gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt atgcctggta
      601 tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg gcaatcaggc
      661 gggactgacg gcagatcgta tgctggtcct gtccagagcc gggcaggcgg cagggctgac
      721 gtttaaccag accagcgagt cactgacggc gctggtgaat gccggtgtgc gtggtggtga
      781 gcagtttgag gcgatcagcc agagtgtggc gcgtttctcc tctgcatccg gcgtggaggt
      841 ggacaaggtc gctgaagcct tcgggaagct gaccacagac ccgacgtcgg gactgacagc
      901 gatggcacgt cagttccata acgtgacggc ggagcagatt gcgtatgttg ctcagttgca
      961 gcgttccgga gatgaagccg gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt
     1021 tgatgaccag acccgccgcc tgaaagagaa catgggcacg ctggagacct gggcagacag
     1081 gacagcacgg gcattcaaat ccatgtggga ttcggtgctg gatattggtc gcccggacac
     1141 tgcccaggga atgctggaga aagcagaaaa ggcttttgat gaggcggaca aaaaatggca
     1201 gtggtatcag agccggagcc accggcgcgg taaaacctca gcatttcttg ccaatctccg
     1261 gggagcatgg gaggacagag cgaatgcgca acttgggctt tcagccgcca cgttgcaggc
     1321 cgatcttgaa aaggccagag agatggcagc aaaggactgg gccgagtctg aggcatcacg
     1381 gctgaaatat accgaagagg cgcagaa
//
LOCUS       DAZFVO020000359         1382 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFVO020000359 DAZFVO020000000
VERSION     DAZFVO020000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.366"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..149
                     /locus_tag="ACHKNB_RS26075"
                     /old_locus_tag="pgaptmp_005215"
     CDS             <1..149
                     /locus_tag="ACHKNB_RS26075"
                     /old_locus_tag="pgaptmp_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198533.1"
                     /translation="QRFALKTTPPVGEVQGSGEEIEITVKTNGLRRRVKATRISGCTV
                     YWEV"
     gene            224..496
                     /locus_tag="ACHKNB_RS26080"
                     /old_locus_tag="pgaptmp_005216"
     CDS             224..496
                     /locus_tag="ACHKNB_RS26080"
                     /old_locus_tag="pgaptmp_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198534.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVHMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMNMSSGVIKGNEFNQQG"
     gene            501..794
                     /locus_tag="ACHKNB_RS26085"
                     /old_locus_tag="pgaptmp_005217"
     CDS             501..794
                     /locus_tag="ACHKNB_RS26085"
                     /old_locus_tag="pgaptmp_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198535.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSKEITEGVTATVKIERNTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVKSSIERRSLQNAPDTLQ"
     gene            806..1336
                     /locus_tag="ACHKNB_RS26090"
                     /old_locus_tag="pgaptmp_005218"
     CDS             806..1336
                     /locus_tag="ACHKNB_RS26090"
                     /old_locus_tag="pgaptmp_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HGU4198536.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT          419 a          282 c          383 g          298 t
ORIGIN      
        1 cacagcgctt tgcactgaaa accacgccgc cggtaggtga ggttcagggg agtggtgagg
       61 aaattgaaat caccgttaag accaacggcc tgcgccgccg tgtgaaggcc acccgtataa
      121 gcggatgcac ggtttactgg gaagtatgaa gttgcttcat ggttcggctc tgtcatcagg
      181 tcagacgccg ggtcagttat ccgaacaaaa cgaggaccag aggatgagta aagtcgtacg
      241 cattattttc gaatacaagg agcacgttat ccataaaaac gcggatggaa cagtgcacat
      301 gggggtaagt ctggacatac gttcaaccgg gataaagcag aaaggtgatg gacctgccat
      361 gatttttggg gtggttatgc tcgcggaaag cagagacttt gctgaacttg tggcaatgaa
      421 agccagtgcg ctcatgaaag atatgaacat gagttccggg gttattaaag gtaatgaatt
      481 taatcagcag gggtaattcc atgagcaaag tacgcgttat ttttgaattt gaccacgtaa
      541 tgcatgaagt aaagccagcc ggtaatgata gtaaggaaat cacggagggg gttactgcca
      601 cggtaaaaat cgaacgtaat acagagaaca gaccggcagg accctgtgat gtttatgcac
      661 aaattcttaa atatcacagc cccacaatta ttcagtttct gacagatgaa ctacagggtt
      721 ccatgcaggc tatgggggtg aaaagcagca ttgagcgccg ttctttgcag aacgcaccag
      781 atacattgca ataaggaaaa acaaaatggc taaacgcgtt acaaaattaa aggccgcagc
      841 cgaggctgca ccacagaccc gtgaagaggt tagccgcgat atccgcaccc tgggcgatat
      901 tcagcgagag gcgctgcgcc tggaaacggc aatgaatgat gaagtggcag aaatcaccgc
      961 ccgttatacg ccgcagattg aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat
     1021 tcaggactgg tgtaaaacca accgcgatga gctgacgaac ggcggcaaaa caaaaactgc
     1081 caatctgacc accggaacgg tgtcatggcg gctgggaacg ccatcatgca gcgtcagccg
     1141 tgatgtggaa ggtgtgattg aaatgctgcg ccgtatgggc cttgagcgtt tcatccgcac
     1201 gaaagaggaa gtgaataagg aagcggtcct ggcggagccg gatgcggtga aagggattgc
     1261 cggtattaag gtgaataaag gtgctgaaag tttttatgtc gagccttttg aacaggatgc
     1321 cggactgaat aaataacact gcattaatca attaaatatc acttcatttt aattatggcg
     1381 ct
//
LOCUS       DAZFVO020000360         1368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFVO020000360 DAZFVO020000000
VERSION     DAZFVO020000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.367"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1368
                     /locus_tag="ACHKNB_RS26095"
                     /old_locus_tag="pgaptmp_005219"
     CDS             <1..>1368
                     /locus_tag="ACHKNB_RS26095"
                     /old_locus_tag="pgaptmp_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4198537.1"
                     /translation="TQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAAS
                     RTLTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWG
                     LSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPP
                     AVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLVTSAITADTEH
                     RFSGLPLGEYTLTVRAINSYGQQGEPATTAFRINAPAAPASIELTPGYYQITAVPVLA
                     VYDPTVQSEFWFSEKRITDMAQVETFARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNL
                     VGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTIT
                     ETRNEITQTVSKTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGA
                     GIEDA"
BASE COUNT          318 a          375 c          432 g          243 t
ORIGIN      
        1 aacgcagacg gtggatttca cgctcgggtc acaggggctg cggcacacgc ccggtgacat
       61 cattgaaatc tgtgataacg actacgcagg gaccctgacc ggcggacgca tcctgtccat
      121 cgatgccgcc agccgtaccc tgacgctgga ccgtgaggtg acactgccgg aagcagggac
      181 atcgacggtg aacctgatta acggcagcgg taagccggtg cgcgtggaca tcactgcaca
      241 ccccgccccg gaccggatac aggtcagcgc cctgccggat ggcgtggaga catacggtgt
      301 gtggggactc tccctgccgt cactgcgtcg tcgcctgttc cgctgtgttt ccatccggga
      361 aaacacggac ggcacctttg ccattacggc agtgcagcac gtaccggaaa aagaagccat
      421 cgtggataac ggggcccgct ttgagccgct ctccggttca ctgaacagcg tcatcccgcc
      481 ggcagtgcag cacctcacgg tggaggtgag tgcctcagac ggccagtatc tggcgctggc
      541 aaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgcc tgaccagtgg
      601 cagtggtgaa aacagccgcc tggtgaccag cgccatcact gccgacacgg agcaccgttt
      661 cagtggcctg cctctggggg aatacaccct gacggtcagg gcgataaaca gctacggcca
      721 gcagggcgaa cctgccacca ccgcgttccg gattaatgcg cctgcagcac cggccagcat
      781 tgagctgacg ccggggtatt atcagataac agcagtaccg gtgctggcgg tgtatgaccc
      841 gacggtacag tctgaattct ggttctcaga aaaacgcatc acggacatgg cacaggtgga
      901 aacctttgcc cgttatctgg ggacaggcag ccagtggagt gtctccggcc cgcacattaa
      961 gccggggaag gatttctggt tttatgtgcg cagcgtcaac ctggtgggga aatctgcgtt
     1021 tgtggaagcc agtggccggg cgagcaatga tgcggaaggg tatctggact ttttcagagg
     1081 agaaatcggg aagacacatc tggcacaggg gatgtgggag ctgattgata acagccagct
     1141 tgacgatgag atggcggaga tgaagaccac catcaccgaa acccgcaatg aaatcacgca
     1201 gacggtcagt aaaactctgg aagaccagag tgccaccata cagcagatac agcgggtgca
     1261 gacagacaca aataacgacc tggctgcgct gtacatgctg aaggtgcaga aaacaaaaaa
     1321 cggcattccg tatgttgccg gtataggtgc ggggattgag gatgctga
//
LOCUS       DAZFVO020000361         1361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFVO020000361 DAZFVO020000000
VERSION     DAZFVO020000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.368"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..81
                     /locus_tag="ACHKNB_RS26100"
                     /old_locus_tag="pgaptmp_005220"
     CDS             <1..81
                     /locus_tag="ACHKNB_RS26100"
                     /old_locus_tag="pgaptmp_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HGU4198538.1"
                     /translation="YGDYYDVDYYDNNLKNEVFDWIGKWS"
     gene            263..1361
                     /locus_tag="ACHKNB_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /pseudo
     CDS             263..1361
                     /locus_tag="ACHKNB_RS26105"
                     /old_locus_tag="pgaptmp_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          499 a          183 c          322 g          357 t
ORIGIN      
        1 tatggagact attacgatgt tgattattat gataacaatt tgaaaaatga agtttttgac
       61 tggattggta aatggtcata atgaacaaca ccagaaagag aaaaatataa aatacataca
      121 gaaagacatg agagcaaggg acggggcagg gcaagatcat gaaaattgtg atgtaaatca
      181 cgattttcat cttggcttta acgcctacag gtgatcaaaa tttaaccttt ggaataacta
      241 aaaagataaa aaaggacgcc aggtgagtat tcaaagtttg cttgattata tttcagtgat
      301 ccctgatata cgacaacaag gaaaggttaa acataaatta tctgctattt tgtttctcac
      361 cgtatgtgca gtaattgcag gtgccgatga gtggcaggaa attgaagatt ttggacatga
      421 aagacttgaa tggctaaaga aatatggtga ttttgataat ggcattccgg tcgatgacac
      481 cattgcacgc gttgtgagta acattgacag tttggccttt gaaaagatgt ttattgaatg
      541 gatgcaggag tgccatgaaa tcactgatgg tgaaattata gcaatagatg gaaagaccat
      601 aagaggctcc tttgataagg gaaaaagaaa aggagcaatc catatggtga gtgcattctc
      661 gaacgaaaat ggtgttgtac tggggcaggt gaaaacggaa gccaaaagta atgagattac
      721 agccattcca gagttgctta acctactgta tttaaagaaa aatttgataa ccattgatgc
      781 tatgggctgt cagaaagata tcgcttcgaa gatcaaagat aaaaaagcag attatcttct
      841 ggcagtaaaa ggcaatcagg ggaaattaca tcatgcattc gaggaaaaat ttcctgtaaa
      901 tgtgttttct aattataaag gcgattcgtt tagtacgcag gagataagtc atggaagaaa
      961 agaaacacgt ttgcatattg tcagtaacgt aacgcctgaa cttttgtgat tttgaattcg
     1021 aatggaaggg attaaaaaag ctttgtgtag cattgtcatt caggcagaag aaagaagata
     1081 aatcagcaga aggtgtaagc atccgatatt atatttcatc aaaggatatg gatgctaaag
     1141 aatttgcata tgctatcaga gcgcactggc tgatcgagca cagtcttcat tgggtgttag
     1201 atgtaaaaat gaatgaagat gccagccgga taagaagagg aaacgcagcc taaataatat
     1261 ctggaataaa gaagatggca ctgaatttat taagggattg caaagacatt aagggtgaag
     1321 aggaaaagaa agaaggctgc gttaaacaca gggaaagatc a
//
LOCUS       DAZFVO020000362         1352 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFVO020000362 DAZFVO020000000
VERSION     DAZFVO020000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.369"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..152
                     /locus_tag="ACHKNB_RS26110"
                     /old_locus_tag="pgaptmp_005222"
     CDS             <1..152
                     /locus_tag="ACHKNB_RS26110"
                     /old_locus_tag="pgaptmp_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HGU4198539.1"
                     /translation="KAAGLAGGVRFGPDVNEVIPVSPDEADMTEDDVMLMTVSEGIAG
                     GVRYG"
     gene            145..>1352
                     /locus_tag="ACHKNB_RS26115"
                     /old_locus_tag="pgaptmp_005223"
     CDS             145..>1352
                     /locus_tag="ACHKNB_RS26115"
                     /old_locus_tag="pgaptmp_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HGU4198540.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSSTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQ"
BASE COUNT          307 a          332 c          459 g          254 t
ORIGIN      
        1 agaaagcggc agggcttgcc ggaggcgtcc gctttggccc ggacgtgaat gaagttatcc
       61 ccgtttcccc ggatgaggcg gacatgacgg aggatgacgt aatgctgatg acagtatcag
      121 aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt cgttgattta
      181 agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca tttttccagt
      241 acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag ccgacaggcg
      301 ctggctgcac agaaagcggg gatttccgtc gggcagtata aagctgccat gcgtatgctg
      361 cctgcgcagt tcaccgacgt ggccacgcag cttgcaggcg ggcaaagtcc gtggctgatc
      421 ctgctgcaac agggtggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
      481 gggcttgccg gtgcgatcac cctgccgatg gtcggggcca cctcgctggc ggtggcgacc
      541 ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt caacaaaacg
      601 ctggtccttt ccggcaatca gtcgggtctg acggcagatc gtatgctggt cctgtccaga
      661 gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag cgcactggtt
      721 aaggcgggag taagcggtga ggctcagatt gcatccatca gccagagtgt ggcgcgtttc
      781 tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa gctgaccaca
      841 gacccgacgt cagggctgac agcgatggca cgccagttcc ataacgtgac ggcggagcag
      901 attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt gcaggcggcg
      961 aacgaggccg cgacgaaagg gtttgatgac cagacccgcc gcctgaaaga gaacatgggt
     1021 acgctggaga cctgggcaga caggacagcg cgggcattca aatccatgtg ggatgcggtg
     1081 ctggatattg gtcgtcctga taccgctcag gagatgctga ttaaggcaga ggctgcgttt
     1141 aagaaagcgg acgacatctg gagtctgcgc aaggatgatt attttgttaa cgatgaagcg
     1201 cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc cgcgagaaag
     1261 aaggctgaac agcagagtca acaggacaaa aatgcgcagc agcagagcga tactgaagcg
     1321 tcacggctga aatataccga agaggcgcag aa
//
LOCUS       DAZFVO020000363         1345 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFVO020000363 DAZFVO020000000
VERSION     DAZFVO020000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.370"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(307..840)
                     /locus_tag="ACHKNB_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /pseudo
     CDS             complement(307..840)
                     /locus_tag="ACHKNB_RS26120"
                     /old_locus_tag="pgaptmp_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            931..1296
                     /locus_tag="ACHKNB_RS26125"
                     /old_locus_tag="pgaptmp_005225"
     CDS             931..1296
                     /locus_tag="ACHKNB_RS26125"
                     /old_locus_tag="pgaptmp_005225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198541.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
BASE COUNT          323 a          357 c          304 g          361 t
ORIGIN      
        1 gaatctacta tcaagagcac attatttgaa aaagatcaca aatttaattc acctcatgtc
       61 cactttgata aaaatataaa actttgtgtc aaagacttgt tacgagatag agtcagacaa
      121 tacaatgaca atggacatta gttttcgatg aaaggcagct cgcaatcgcc tcattcagta
      181 tgaaacccga acttactccc acactacagt accacaaaaa aagcatccct agatgggagt
      241 gtagttatcg tctcttcgtc cacttttttc atcatcggtg agttgcacta tcttcccctg
      301 tagtggtcaa caaaaactgg ccaccgcttt agagtttttc cagtatcggt tttccgattc
      361 atttggtggc aacccaccgt tatattcgtg cggcctgagc gcgttgtaat acccaacgat
      421 atagtccgtt atttcatggg cagcatcgct gaagttcatg taacccgtca ccggtatcca
      481 ctcgtttttc agactcctga agaagcgctc catcgggctg ttatcccagc aatttcctcg
      541 tcgactcaga ctctgtttga tctggtaacg ccacagtaac tgccggaact gcctgcttgt
      601 atagtggctg ccctgatcgc tgtggaacat taccccggct ggtttactgc ggatttccca
      661 ggccattttc agcgctttga tggtcagtct gctgtccgga gagaacgaca ttgcccaacc
      721 taccggtttc cttgcaaaca ggtcgagaac aacggcaagg tatgcccaac gtttccccgt
      781 ccagatgtac gtcacgtcgc cgcaccatac ctgatttggc tctgtcactg cgaactgccg
      841 cccgagtagg cagcctggcg gctgcggctt gtcatggcct gaaattaccg ttataaaaac
      901 agacaatatc attgtctttc aggtagttat atgtcccgtt cagctaaacc ccgtaaacga
      961 aaacctgccc ctcaaagaag caaacttccc cgctatgtcg tgaagcttca cgacgatgac
     1021 ttctttgacg aagaagacgc agaagctctg cgctttgata attttgacga tgccgttgag
     1081 tgctgcgcag acctgaatat tcccttcttt gtggatgccg gaaacaaaaa gctggtcttc
     1141 tggtttgttc gtgttgatga cgaagggtat cctgaaatag cccgctgcac ggagcgggag
     1201 tttgcgacca ttcttgccgg tatcagcgcc ggcggcatgt actgcccgga gtgtggcacg
     1261 gttcactggc cggacggagt ccccccgccc ttctgatgct tccccgtttt gccgacattt
     1321 ttcagcaggg aaaccgctgg cttaa
//
LOCUS       DAZFVO020000364         1338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFVO020000364 DAZFVO020000000
VERSION     DAZFVO020000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.371"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            192..>1338
                     /locus_tag="ACHKNB_RS26130"
                     /old_locus_tag="pgaptmp_005226"
     CDS             192..>1338
                     /locus_tag="ACHKNB_RS26130"
                     /old_locus_tag="pgaptmp_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198542.1"
                     /translation="MSKNLPVLLSSPSTTNFPATVNTEIEFLSQARKLLDSGFPDHAL
                     LDIWNASIHNLRRRIEAYGLDLFLSAIKDDSGRKKYDKDGETINERWSGVDDLVLISG
                     ATKLGVLHKKAGKSLEMINWMRNHASPAHASDSKVESEDVFALALMLQKNLFESDMPD
                     PGHSPSGLFEPIKKSELSIESIDLLKDQIRAFKQADIRITFGFLLDLITKGENPAYVN
                     ASKLFEQAWERSNDDLKKTAGERYHSFMLEPESDTSEDKGAKIRILETLVKVNGVKFI
                     PDAARASLYRHAVKQLAKAKDTSYGWNDEERAAKALIQFGPHVPSIAFEDVYQEILTV
                     WVGNYWGRSDAHLVLQPFLDVLNSNQLMALSRLFVENERANSELVSSR"
BASE COUNT          449 a          201 c          276 g          412 t
ORIGIN      
        1 tagttttgaa aattaatttt ttaaacatat tgaatttttt aagaattggg aatagtgtat
       61 agctttgtca tggcggattt tttctcttca agtatttatt tgaacactac ttcttgcacc
      121 tgcaaggtta caatatcgtg taaatttgtt cacaaagaca tagattataa tttttactta
      181 aaggaaattc aatgtcaaaa aatctcccgg ttttattaag ctcgccatca acaactaatt
      241 ttcctgcaac agttaatact gaaatagaat tcctttcgca agctagaaag ttgttagatt
      301 ctggcttccc tgatcatgct cttttagata tatggaatgc atccattcat aatcttaggc
      361 ggcgaattga ggcttatggt ttagacttat tcctttccgc aataaaggat gatagtggtc
      421 gtaaaaaata tgataaagat ggtgaaacaa taaacgagag atggtcaggt gtagatgatt
      481 tagtgctaat aagtggagcc accaaattag gggttctgca taagaaagct ggaaaatcat
      541 tagaaatgat caattggatg agaaaccatg cctctccagc ccatgcaagt gatagtaaag
      601 tagagtctga ggatgtgttt gctttggctc ttatgctcca gaagaattta ttcgaatctg
      661 atatgccaga ccctggacat tcgccatctg gattatttga acccataaaa aaatctgaac
      721 tatcaattga gagcatagac ttacttaaag atcaaataag agcatttaag caagctgata
      781 taagaataac atttggattt ttattggatt taataactaa gggggaaaat cctgcttatg
      841 ttaatgctag caagttgttt gaacaagcat gggaaagatc taatgatgac cttaaaaaaa
      901 cagcggggga aagatatcac tcatttatgc tggagcccga atcggataca agtgaggata
      961 aaggagcaaa aataagaatt ttagagactt tagtaaaagt taatggtgtg aaatttatac
     1021 ctgatgctgc tagggcaagt ttatacagac atgcggttaa acagctagct aaagctaagg
     1081 atacatctta cggctggaat gatgaagaaa gagcagccaa ggcacttata caatttggac
     1141 ctcatgttcc atctattgct tttgaagatg tatatcaaga aatacttact gtttgggtgg
     1201 ggaattactg gggaagatct gatgcacatt tagtacttca accattttta gatgtgttaa
     1261 atagtaatca attgatggct ctctcacggt tgttcgtgga gaatgaaaga gcaaattcag
     1321 aactggtgtc atcaaggc
//
LOCUS       DAZFVO020000365         1326 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFVO020000365 DAZFVO020000000
VERSION     DAZFVO020000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.372"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(209..>1326)
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS26135"
                     /old_locus_tag="pgaptmp_005227"
     CDS             complement(209..>1326)
                     /gene="ompC"
                     /locus_tag="ACHKNB_RS26135"
                     /old_locus_tag="pgaptmp_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HGU4198543.1"
                     /translation="AVMFAAGAQAAEVYNKDGNKLDLYGRATALHYFSDDKGNDGDQT
                     YARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAFAGLRFADVGTIDY
                     GRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNTDFFGLVDGLNFAA
                     QYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQVQGLNGTADVLDP
                     ASGKKVGEKAVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSETQNMTTFGDQGVAD
                     KAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVKYIDVGATYYFNKN
                     MSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT          398 a          349 c          246 g          333 t
ORIGIN      
        1 tatccgccca taaaaaaggg gcgcagacgc gccccgaaca ataaaagtcc aatttaaaat
       61 aaaaccaata aaggtaataa cagaaagtta aatcagcaca tgccagatgg caattgctga
      121 tcgcagtgac caatgaaaac tgtgttttca ttgatacagg ccgcctgtaa gggcagacgg
      181 cccgtattta ttaacttatc agcaataatc agaactgata agtcataccc acagcaacga
      241 tattatctgt cgcaacaccg gctttccggg taaattcact ttcatcaatc aggttaattt
      301 tataatcaac ataggtggac atattcttgt taaagtaata agtcgcaccg acatcaatat
      361 atttaaccag atcctgatcg ccatggttca caccaccaac cataacatcc tgtccgcggg
      421 actgaaggta ggcaagggac gggcgcagac caaaatcaaa ctgatactga acaacggctt
      481 cgatattctg agctttatcc gcaacacctt gatcaccaaa ggtcgtcata ttctgggttt
      541 cagaatacac tgctgccaga tacaggttat tggcatcata tttaagacca gttccccaga
      601 attcagcatg tttacctttt gccacactac ctgagtcaac tgctttttct ccaacctttt
      661 tacctgatgc aggatcaaga acatctgcag taccattcaa cccctgaacc tgcttatcag
      721 tacgatctga cttggtatac gcagcgacaa aaccaaaacc atcaaactca tagctggccg
      781 agaaaccata cccgtcgcca ttagccttct gaaggtcatc acgctcattt ttgccctgat
      841 actgcgccgc aaaattcaga ccatcaacca gtccgaagaa gtctgtatta cggtaagtca
      901 ggacaccgga agttcgggcc gtcataaagt tatcggtctg cgtccagcca tcaccaccaa
      961 actcaggcag tacgtcagta tatgatccga catcgtaagc aataccatag ttacgtccgt
     1021 aatcaatggt accaacatct gcaaatctca gacctgcaaa tgccagacgg gttttatttc
     1081 ccgcggaacc ttcagattcc gttttgttac cagagaactg gtattcccac tgaccaaatc
     1141 cggtcagttg atcattaatc tgcgtttcgc ctttaaagcc gagacgagca taagtctggt
     1201 ctccgtcatt acctttatca tccgagaagt aatgcagagc ggttgcacgc ccgtaaagat
     1261 ccagtttatt accatcttta ttataaactt ctgctgcctg cgcccccgca gcaaacatca
     1321 ctgcca
//
LOCUS       DAZFVO020000366         1305 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFVO020000366 DAZFVO020000000
VERSION     DAZFVO020000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.373"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..1232)
                     /locus_tag="ACHKNB_RS26140"
                     /old_locus_tag="pgaptmp_005228"
     CDS             complement(81..1232)
                     /locus_tag="ACHKNB_RS26140"
                     /old_locus_tag="pgaptmp_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HGU4198544.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDAAENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKEIATSVGSWSAARGQQSVALGGGTYAYADASTA
                     LGSIAFVDNTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT          325 a          364 c          269 g          347 t
ORIGIN      
        1 cacctgtttc atgctcacat gagaaaaaac ggcagacaaa actccacgcc tgccgtcatg
       61 cttcatgtca ctgtcagcaa ttaaaactca aagttcacac cagcgttcca ggatacatcg
      121 ccatcgctcg ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca
      181 cccacggcaa cagcctgttt gtcactgtaa ccaccaacgg cagcagtggc gttgaattta
      241 cccacactgt acggctggaa aagacctgcc agcgctgcgc tctgggctgc cgcacgtttc
      301 atctcttcgt ggttttcatt aatctgacgc tgatgactgt tcagacgtgt gttgtgctcc
      361 tgcaactgac gggaagtgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaaca
      421 ctaccaacag tagcatcaat acggtctgtt ctgccggaca aagctacaag cccatcatta
      481 acagccaggg ttttgctggc tccttctgca cgggtcaggg ttccgtcgga gttaacacca
      541 actgtcgttc tggagtctgt atattcacga gactttttcg cttcaaccac aaggcgggta
      601 ttcgtatcct gagccagttg ctcaacactg tcttttacac ggtcaacctg acgaacggtt
      661 gcagcatctg agggacgagt accgtcactc agaccaccaa gcacccgggt tttataagcc
      721 ttacccggct ctgcttcata accgatataa acctcattgt cacgggttgt gcgtgaatta
      781 gctcctaatg caacactgtt tttatttgtt actgttgcct ttgcccctaa cgcagtacct
      841 tctgttgcat ctttatcaac tttggctcgg ttacctgctg ccgtgccata tgtagccgtg
      901 ttatcaacaa atgcaatgct tcccagggct gtagatgcat ctgcataagc atatgttccc
      961 ccacctaaag ccacactttg ctgtcctcgt gctgcagacc atgatcccac tgatgttgca
     1021 atttctttat ccgcgctggc ccctgtacca acagctagcg caatctctcc atccgcgcga
     1081 gctgaagcag aaagatctgt attttctggg atactccctg aggcgataag cttattctcc
     1141 gctgcatcct gagccagaac gccagcgctt acaccaaata aaataccgcc aataacaacg
     1201 gaaataacac ttcgtgataa agacttatcc atgtatgatt cctcatgaat ttatttgtat
     1261 acaaacaaac cgaaatacat cggatgaata aagaaaatct gatgt
//
LOCUS       DAZFVO020000367         1304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFVO020000367 DAZFVO020000000
VERSION     DAZFVO020000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.374"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            119..790
                     /gene="queE"
                     /locus_tag="ACHKNB_RS26145"
                     /old_locus_tag="pgaptmp_005229"
     CDS             119..790
                     /gene="queE"
                     /locus_tag="ACHKNB_RS26145"
                     /old_locus_tag="pgaptmp_005229"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HGU4198545.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          360 a          275 c          298 g          371 t
ORIGIN      
        1 tattaacgat atgtttcgca tttttcttta aatctgcctg taacagaatg ttaataagac
       61 atgattgttg tggcaggatc tgcaataatt aacaattatc cacttcacag agaatgctat
      121 gcagtacccg attaacgaga tgttccagac cctgcaaggt gagggttact ttaccggcgt
      181 ccccgccatt tttattcgtt tacagggatg cccggttggc tgtgcctggt gcgacaccaa
      241 acacacctgg gaaaagcttg aggatcggga agtctccctt ttcagcattc tggcgaagac
      301 caaagagagt gataagtggg gggcggcgag cagtgaagat ttgctggctg tcattggtcg
      361 gcagggatat accgcgcggc atgtggtgat tacgggtggt gagccttgca ttcatgattt
      421 gctgccactg actgacctgc tcgaaaagaa cggttttagc tgccagatcg aaaccagtgg
      481 aactcatgaa gtacgctgca caccgaatac ctgggttacc gtatcgccaa agctgaacat
      541 gcgcggcggc tatgacgtgt tgtcacaggc actggagcga gccaacgaaa tcaagcatcc
      601 agtggggcgc gtacgcgata ttgaagcact ggatgaacta ttggcaacgc tgaccgatga
      661 taaaccgcga gtcattgcac tgcagccgat tagccaaaag gatgatgcca cacgtttgtg
      721 cattgaaacc tgcattgcgc gtaattggcg tttgtcgatg caaacacata aatatctaaa
      781 tattgcctga ttaaacattt ataagcgtta taaatgggtg gaacctgtaa agacttctac
      841 tcatttatat tgtttgtcgc ctctgaaaac tcctccattt tacccatcca gggctaatca
      901 ttagcattct ctacaaattc tgtggcatta atttttcgct ggagtgaaaa ttattgcggt
      961 aaagtttggt agattttaat ttgtatagag ttattttaaa tatttacctt tttaatcaat
     1021 gaattaagta ctctttaaca taatggatgt gttgtttgtg tgatactgta aagttggtag
     1081 attgtgactg gcttaaaaaa tcattaatta ataataggtt atgtttacag agttccccgc
     1141 gccagcgggg ataaaccgcc gcttaaccgg acctcatcac aaaacacgcg gagttccccg
     1201 cgccagcggg gataaaccac catataaccc gttatctctt tctcaagttt ttatattagc
     1261 agtacttgta ataagcaaca tatccacgta acacctcatg ttca
//
LOCUS       DAZFVO020000368         1303 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFVO020000368 DAZFVO020000000
VERSION     DAZFVO020000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.375"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..222)
                     /locus_tag="ACHKNB_RS26150"
                     /old_locus_tag="pgaptmp_005230"
     CDS             complement(<1..222)
                     /locus_tag="ACHKNB_RS26150"
                     /old_locus_tag="pgaptmp_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGU4198546.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKR"
     gene            complement(219..443)
                     /locus_tag="ACHKNB_RS26155"
                     /old_locus_tag="pgaptmp_005231"
     CDS             complement(219..443)
                     /locus_tag="ACHKNB_RS26155"
                     /old_locus_tag="pgaptmp_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGU4198547.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            509..864
                     /locus_tag="ACHKNB_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /pseudo
     CDS             509..864
                     /locus_tag="ACHKNB_RS26160"
                     /old_locus_tag="pgaptmp_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1097..1228)
                     /locus_tag="ACHKNB_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /pseudo
     CDS             complement(1097..1228)
                     /locus_tag="ACHKNB_RS26165"
                     /old_locus_tag="pgaptmp_005233"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT          383 a          326 c          266 g          328 t
ORIGIN      
        1 tcgcttattt gcatcgttga aaatgtgacc acgagcaata gcaactaaat acacggcagc
       61 cagatccata atatctgtca caccttcata atgatacatg ttcaaaacac gatgaatgat
      121 cgcgtcagcg cgaccgggtt ctggcatacc cgccacgcca ccatcacgcg aaatcaagcg
      181 gtcatgaaac tcaatcattt cctgtgaact aacgaatttc atcgcttaga aagctccgta
      241 taggccgtcc catgactttg catgatctca gcaaactcag catcaagagc cgctttacgg
      301 tatcgttcaa aagcgtcttt atcaaccagt acagccgcgc cacctttacg gcgctgaatc
      361 tcaacaggct gattttcatc aaccgccagc tcgagcaacg aggccaggtt atcgcgagca
      421 tggctatagt tacatgtaaa catagtgctt cctctttgag ttgtacaatt aaattgtaca
      481 acagttatct tcaatatgaa atcaccgtta ccgctggcgc atcctgaccc aacttaaccg
      541 gggagaaagt cgccatgccg ttgccagagc tatctgtcac ggtcagaaag gtgagataag
      601 aaagcgatat accgatggtc aagaagatca actgggcgca ctggggctgg tgactaacgc
      661 cgtcgtatta tggaacacta tttatatgca ggctgccctg gaccatttca gggcacaggg
      721 ggaaatcgtg aatgatgaag atatcgcacg cctctcacca ctttgccacg gacacatcaa
      781 tatgctcggc catattcctt cacgctggca gaactggtga ctaaagggca aaaagaggca
      841 tcggaggaag aaaacattgc ttaacgtgag ttttcgttcc actgagcgcc agccccctaa
      901 ttctctatat ccaaactgac cccaataggt gacgggttat gtgacgaaat ctgatgtaga
      961 aatcgttgtt tcagtgacag tcactcaatc ggtcatttat gtgacacccc acgccgttac
     1021 tggtcgcgga aaaatccagc ggaaaaatcc aggtttccgg cttgggaccc catctcaaga
     1081 gatcctttga tcttttctac tgaaccgcga tccccgtcag ttcagaagac gaagatgacg
     1141 caacggttcc tcattgtaca tcacgaatat agtttgcttg acatcctgac aagaataata
     1201 ttttaattag aaaaattatt attttcatat ccctttccga caaaagtaaa aatacttatg
     1261 catttaaaat actacctaca taatttacct gaatcactta tac
//
LOCUS       DAZFVO020000369         1279 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFVO020000369 DAZFVO020000000
VERSION     DAZFVO020000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.376"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..210
                     /locus_tag="ACHKNB_RS26170"
                     /old_locus_tag="pgaptmp_005234"
     CDS             <1..210
                     /locus_tag="ACHKNB_RS26170"
                     /old_locus_tag="pgaptmp_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198548.1"
                     /translation="TKDQNATAVNARRHGDFAVALCMANRASYMEGFILDESACQALP
                     ERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            210..>1279
                     /locus_tag="ACHKNB_RS26175"
                     /old_locus_tag="pgaptmp_005235"
     CDS             210..>1279
                     /locus_tag="ACHKNB_RS26175"
                     /old_locus_tag="pgaptmp_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HGU4198549.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHA"
BASE COUNT          264 a          343 c          406 g          266 t
ORIGIN      
        1 acgaaggacc agaacgcaac ggctgtgaac gcccgccgcc atggcgattt tgccgtggcg
       61 ctctgtatgg caaaccgggc gtcatacatg gagggcttca tcctggatga atcagcctgt
      121 caggcgctgc cggagcggtc gcgggcaatg gagggcggtt atcgtgatga cgatgaggca
      181 tatcatgaat ttgatcgggg gtgctggtag tgggacgcat aattgatctt gacggaaaac
      241 ctttctcctt tgacccggag atgcagagtg ccgcgctgga tattccgcag attgccagcc
      301 gttatattga acatccggcc tcgggtatca ccccgaaccg ggcggcacag tgccttcgcg
      361 gagctgaacg tggcgatctg attgcccagt ccgatctggc cgctgacatt gaagaaaagg
      421 atacccacct ttttgcagag ctgggcaagc gacgccttgc cattcagggg gtgccatgga
      481 gtattgagcc gcccccgaac gccagcgcga atgagaaaaa ggacgcggaa atgctcgacg
      541 aatatctgca ttccgccgac tggtttgatg ccatgctgtt tgatgccacc gatgccatcc
      601 tgaagggcta ttcctgtatg gagattgagc acgggatgct cggtaaaatg cacatcatcc
      661 gcgccatccg ctggcgtgac agcgggcatt tctgccttaa cccggatgat ttgagcgaac
      721 tgcggctgcg tgacggcagc catgccgggg tggcgtttca gccctttggc tggatagtgc
      781 atcagtcgcg ttcacgcact ggctacggtg gtgcgacggg gcttgtcaga acgcttatct
      841 ggccgttcat tttcaaaaac tattccgtgc gcgatctggc tgaatttctg gaggtgtacg
      901 gcctgccgat gaaggtaggt aaatacccgt ccggggcaac gccggagcag aaaagcgccc
      961 tgatgcgggc ggtgatggat atcgggcgac gtacaggcgg gatcatcccg gccgggatgt
     1021 cgctggagtt tcaggcagcc gcgaacggtc aggccgatcc gtttgaaacc atgatttcgt
     1081 ggggggagcg ttccatctcc aaagctatcc tcggcggcac gctgaccacg gaagccggag
     1141 acaagggcgc acgttcgctg ggtgaagtgc ataacgaggt gcgccgggaa atcagggatt
     1201 ctgatttacg ccagctggcc gccacgctga accgcgatct ggtgtatccg ctgtatgccc
     1261 tgaacaccgc ccacgccat
//
LOCUS       DAZFVO020000370         1274 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFVO020000370 DAZFVO020000000
VERSION     DAZFVO020000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.377"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..72
                     /locus_tag="ACHKNB_RS26180"
                     /old_locus_tag="pgaptmp_005236"
     CDS             <1..72
                     /locus_tag="ACHKNB_RS26180"
                     /old_locus_tag="pgaptmp_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HGU4198550.1"
                     /translation="ATLRSVSYQNELLLEQAVRKKTQ"
     gene            69..359
                     /locus_tag="ACHKNB_RS26185"
                     /old_locus_tag="pgaptmp_005237"
     CDS             69..359
                     /locus_tag="ACHKNB_RS26185"
                     /old_locus_tag="pgaptmp_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4198551.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            362..943
                     /locus_tag="ACHKNB_RS26190"
                     /old_locus_tag="pgaptmp_005238"
     CDS             362..943
                     /locus_tag="ACHKNB_RS26190"
                     /old_locus_tag="pgaptmp_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4198552.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            943..>1274
                     /locus_tag="ACHKNB_RS26195"
                     /old_locus_tag="pgaptmp_005239"
     CDS             943..>1274
                     /locus_tag="ACHKNB_RS26195"
                     /old_locus_tag="pgaptmp_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198553.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISA"
BASE COUNT          292 a          343 c          391 g          248 t
ORIGIN      
        1 gccacgctcc gctctgtctc ctatcagaac gaactgctgc tggagcaggc tgtaaggaaa
       61 aaaacgcaat gagtgatttc attactgaag atcagcgtct ggtcattctg cggtcgctgg
      121 cagattacaa cggtgaactg ggtgaatccg tgctccagga ctgtctggat gattacggcc
      181 accgcgtgtc ccgcgacacc gttcacaccc acattgcctg gcttgccgag cagggactgg
      241 tgcgtaagcg cacgctcgtt aacggttatt tcatcgccga actgaccggc cggggtcagg
      301 atgtggcgga aggtcgcgcc agcgttccgg gtgtgaagaa accacgcgca agggggtaac
      361 gatggacaag ccgacgcgtg gacgcgtgcg caaggtggat ttgctccccg acagcatccg
      421 taagccactg ctggaaatgc tgcgtgaaaa acgtctgacg caggtccaga tacgcgagga
      481 aatcaaccgt ctgatccgtg aggcgggcct gccggaagag cagcagcttt caccggcggc
      541 catcagccgg gaagcctccc gtaatgagct gattgcccgt aacctgcgtg atttacgcga
      601 gcagacaaaa gccatgatgg ccgaactggg agacaagcca accggtgaaa ccacagcgct
      661 gattctggag atgtcccgcg ccctgatgta ccgccgtctg cgtgccgcga ccgaatccct
      721 gaacagtgac agcgacgtgg atatgcgtct tattaaggac atcctcctgt cagcacagcg
      781 tgcggaaagt gccgccgagc gcagcattaa gcgtgaaaaa gaaatccggg ccgcatttgc
      841 tgaagagatg gcaaacgccg tcaccgacga gctgcgcggc gtggacggga tgagtgaaca
      901 gcttgagtcc cgtattaagg ggattctgtt gggtaaagcc tgatgaataa cgccgaagaa
      961 ctctccccct tactgacgaa taccgtctcc acccgcaaaa ttgatctggc cggtgaaaaa
     1021 gccctgctgg gcgtggatgt gccggacagc cttgatttgc ccggtgatat gccggtattt
     1081 ctggattatc aggcccgctg gtttgaggat gaaagcgagg tctgcatcgc ggaaaaatcc
     1141 cgccgcaccg ggcttacctg ggcggaagcc gggcgtaacg tcattaccgc cgcaaagcct
     1201 aagcggcgtg gtggccgcaa tgtgttttat gtggggtcaa agcaggagat ggcgctggaa
     1261 tatatttctg cctg
//
LOCUS       DAZFVO020000371         1263 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFVO020000371 DAZFVO020000000
VERSION     DAZFVO020000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.378"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(19..360)
                     /locus_tag="ACHKNB_RS26200"
                     /old_locus_tag="pgaptmp_005240"
     CDS             complement(19..360)
                     /locus_tag="ACHKNB_RS26200"
                     /old_locus_tag="pgaptmp_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198554.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(353..>1263)
                     /locus_tag="ACHKNB_RS26205"
                     /old_locus_tag="pgaptmp_005241"
     CDS             complement(353..>1263)
                     /locus_tag="ACHKNB_RS26205"
                     /old_locus_tag="pgaptmp_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198555.1"
                     /translation="ARASNDAELLGYGQGERVRERMRELQQIRDGYRQKDADLQSQYQ
                     TGDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGLQEGLSNWVDSASD
                     YASQAAQLATDGISGMVNNITEMLNGNKVEWRSWAASILQEISKVLMNAAIVNGIKMA
                     ANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLM
                     GEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANGQIGPEAAG
                     TLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          256 a          432 c          276 g          299 t
ORIGIN      
        1 cttcgtgaat atcctgcatc agttcaccac ctgctctatc gtgcaactga aatcactgta
       61 ccgggcgtta tccgtaatac tccactcccg gcacaccacc ctgaccgtcc ggttatgttt
      121 cgggggcttc cacaaaaaag cccggtaacc accatgccat gacagaaacg cggacagtgc
      181 ctcccgctca gcatccgttg tcacccgaaa aaccacctga aaggtcttca gttgcgcatt
      241 cagtcctgcc ggacgacgtt gctgataacc gtcgccaaac ttcaccgtca ccaccgacgg
      301 tttctccgtc acctgcatcc cttcccgggg acaccagtga agggtcttaa tctcatccac
      361 tcagcattcc tccgtcacga cgcatggata acatcaccgc ctgtacccgc tggtcaatca
      421 gttgcacaag ggtgcccgcc gcttccggcc ctatctgccc gttagcgccg tcattctgaa
      481 tggcgatgtg gtacaccggg gaatacacca gacccgcact gccgttcata ctgcccaccg
      541 cacgcacgcc gagtgaacca tccgctgccc gggtcagggg catgatggct tcaggtccgg
      601 cctcccccat cagccctgcc ccttttgcaa aggcaaagta cgtgggcgtg tccacaatac
      661 tgttactgta tgcactcagg tttgccgagg tataaacgcc gccttttgca tttgccaccg
      721 cgccacccag ccagttgcct atactgccga aaaatcctcc tgcaccggac atactgtttg
      781 ccgccatctt aatgccgttg acaatggccg cattcataag aacttttgat atttcctgca
      841 ggattgatgc agcccagctg cgccattcca ctttatttcc gttcagcatc tccgtgatgt
      901 tattcaccat ccctgagata ccgtctgtcg caagctgtgc tgcctgtgaa gcgtaatctg
      961 atgcgctgtc cacccagtta cttagccctt cctgcagccc tttctgccag tcagcacgct
     1021 gcgcatccga ttcggcataa aaagcctcct ggtctttaag gcgttcactc agatactgcg
     1081 cattctgcgc cagcgcctgt ctgtaaaaat cctcactgat atcccctgtc tgatactgag
     1141 actgcaggtc cgcatccttc tggcggtagc cgtcacgaat ctgctgcagc tcccgcatcc
     1201 gttcacggac ccgctcaccc tgcccgtacc ccagcagctc agcgtcattt gatgcccttg
     1261 cgt
//
LOCUS       DAZFVO020000372         1260 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFVO020000372 DAZFVO020000000
VERSION     DAZFVO020000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.379"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..559
                     /locus_tag="ACHKNB_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /pseudo
     CDS             1..559
                     /locus_tag="ACHKNB_RS26210"
                     /old_locus_tag="pgaptmp_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            710..1219
                     /locus_tag="ACHKNB_RS26215"
                     /old_locus_tag="pgaptmp_005243"
     CDS             710..1219
                     /locus_tag="ACHKNB_RS26215"
                     /old_locus_tag="pgaptmp_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HGU4198556.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          345 a          297 c          278 g          340 t
ORIGIN      
        1 tattcctgtt cacgatacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca
       61 cgagtgcttt attaactgga tgcgtgattg ccattcatca aatgataaag acgtcattgc
      121 aattgatgga aaaacgctcc ggcactctta tgacaagagt cgccgcaggg gagcgattca
      181 tgtcattagt gcgttctcaa caatgcacag tctggtcatc gggcagatca agacggataa
      241 gaaatccaat gagatcacag ctatccctga acttcttaac atgctggata ttaaaggaaa
      301 aatcatcaca accgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca
      361 gggaggtgat tatttattcg ctgtaaaagg aaaccagggg cggctaaata aagccttcga
      421 ggaaaaattt ccgctgaaag aattaaataa tcccaagcat gacagttacg caattagtga
      481 aaagagtcac ggcagagaag aaacccgtct tcatattgtt tgcgatgtcc ctgatgagct
      541 tattgatttc acgtttgaat agaaagggca gctatggtta gaaactacct gacgtcagtc
      601 cttgcgggga gcaggctttc gtaaatttgt cctgctacaa caggattaac ttcacaaata
      661 tcatttctca acgtctacac ttactcctgt aaaccgttca ggagcagtaa tgaatcagca
      721 acgatttgat gacagcaccc taatccgtat cttcgccctt catgagttac atcgactgaa
      781 agaacatggc ttaacgcgcg gggcgcttct cgattatcac agccgctata aactcgtctt
      841 tctggcgcat tctcagccgg agtaccgcaa acttggcccg ttcgtggctg atattcacca
      901 gtggcaaaat ctggatgatt tttacaacca gtactaccaa cgcgtaattg ttttgctttc
      961 tcatcccgcc aacccgcgcg atcacaccaa cgttttgatg cacgttcagg gttattttcg
     1021 cccgcatatt gattccacag aacgccagca gttggcggcg cttatcgaca gttatcgccg
     1081 tggcgagcaa ccacttcttg cgccgctgat gcgtatcaaa cactatatgg cgctttatcc
     1141 tgacgcctgg ctttcagggc agcgttattt cgaactttgg ccgcgtgtga ttaacttgcg
     1201 ccattcagga gttttatgat tacccatctg gtctggtttc gccaggattt acgtctgcac
//
LOCUS       DAZFVO020000373         1259 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFVO020000373 DAZFVO020000000
VERSION     DAZFVO020000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.380"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            166..348
                     /locus_tag="ACHKNB_RS26220"
                     /old_locus_tag="pgaptmp_005244"
     CDS             166..348
                     /locus_tag="ACHKNB_RS26220"
                     /old_locus_tag="pgaptmp_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4198557.1"
                     /translation="MTFIQLILLYFCTVVCVLYLVSGGYKAIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPPPL"
     gene            384..629
                     /locus_tag="ACHKNB_RS26225"
                     /old_locus_tag="pgaptmp_005245"
     CDS             384..629
                     /locus_tag="ACHKNB_RS26225"
                     /old_locus_tag="pgaptmp_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4198558.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDSAMM"
     gene            704..904
                     /locus_tag="ACHKNB_RS26230"
                     /old_locus_tag="pgaptmp_005246"
     CDS             704..904
                     /locus_tag="ACHKNB_RS26230"
                     /old_locus_tag="pgaptmp_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198559.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(1091..1201)
                     /locus_tag="ACHKNB_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /pseudo
     CDS             complement(1091..1201)
                     /locus_tag="ACHKNB_RS26235"
                     /old_locus_tag="pgaptmp_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          382 a          270 c          301 g          306 t
ORIGIN      
        1 tggtggaagt gaataacccg tcagcaggat aaaaaaatgc cgccggtaag ggaaaacaag
       61 aaaccggcgg caaatgtcgt catatccaaa gcaaaacatg cagtacgcct ttttaaccaa
      121 caggtattaa cgatgtcaac accatatcaa taattgggag ggataatgac atttatacag
      181 cttattttat tgtatttctg cacggtggta tgtgtcttat atctggtgag cggtggttat
      241 aaggccatca gaaattatat tcgccgtaaa attgatgatg cggcagctga aaaactcagt
      301 aaaacagcac aggcaccgtc atccccaaac gacccgcccc cgctctgata acggggcaat
      361 atacctgcaa aaggagaata ttcatgccag agattaaagg cacggttact gaagagcttg
      421 tcaaacaggc actttattct gaagaagtga accgcgtgct gaaggcgcag gttcgcaagg
      481 attttgaggc acaaatcgac gcatatgttg atgaagtgct ggcccgaatg gttggtcgtt
      541 ctccggctga aaacagcacg gagaatgagc ctcagcccgt agagcaacca gagccggttc
      601 aacccggaac tgacagtgcc atgatgtaac aatacaaacc gacagcttct cctgtcggtt
      661 tgattttcat gggcgatgta tataccagtc agaggaaata atcatggaca agaacaaaaa
      721 gcagcccgat accaaaggtc tggaaatatt aagcgcagaa attatcaatg cgctacgttc
      781 aggacttgat gaaaatacat tacaggatcg aaacttcacc actgcaatgt ggatagcgat
      841 gcagatcctg tgtaccccaa gatgcgctat ttttgcggtt cgtgagggtc agttgactcg
      901 ctgattgccc gaattacatc agaaaagcac gcgttccata attgcatagc tgatgaatca
      961 ccggctgtat gaaaagaggt aaagacacaa ctggtggatg tcattgctgc aattgtgata
     1021 tcagctgcaa ttcgcgcagc ctgtaacttt tggtcggtgg tcagtttatc cattttctcc
     1081 tgcctgaaca gatgcatctc tgctttacag gaggccgggg aatatcatag gatacaccac
     1141 gagctcagag tgaagcattg atgcagtttt ttacagattg acccagaata gttcgcgcta
     1201 tctgagtcgc catttatgta gtcccgaagt aaagcattga tgcaaacttg tctaaatcg
//
LOCUS       DAZFVO020000374         1246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFVO020000374 DAZFVO020000000
VERSION     DAZFVO020000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.381"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(8..1246)
                     /locus_tag="ACHKNB_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /pseudo
     CDS             complement(8..1246)
                     /locus_tag="ACHKNB_RS26240"
                     /old_locus_tag="pgaptmp_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          257 a          385 c          309 g          295 t
ORIGIN      
        1 tcattcattt tttcagaatc cccctgaaca tcgccttcac cgcatcatgc tctgtttcag
       61 tgattgcctt attaccgtca gaggcaacga ccgcctgagg cacggaagct gttttaccgg
      121 acgacgcgaa cggatcttca cgggcatcac ggcgggacaa cgcttccaga ctgtagttct
      181 gctgctgaag atacagtgca tcaccgccgg ccaggggcgg caggttctcc cgtttacggg
      241 cctcattggg cgtgagaagc gtatttttca ccgcatcccc cagcgttttc atgcgccgct
      301 cactgtccat tctcagcagc gtggtgacat caaattccgt actctcgttt tcccccgttt
      361 ccagcgcctc atccagtaac agttcaatgg actcaatcag cgtctgcagg cactgagaat
      421 aatactgctg ctccagcgcc tccacgttgt cactggaagg cggttgtccc acgccaatct
      481 tgtaggccgg gacacggaac accgaacaga caatttcagc ggtcatcttc agttgttcca
      541 ccgtctgcga atccaccggt gaaaacgtcg tggggttgta ttttgccccg ttgctcagaa
      601 tggccgtttt ccccgcattt tcgcctgtat acccgctgtc ccagttgctc ttcagttttt
      661 tcgcattttc ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat
      721 ttctgaaaaa agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg
      781 cggcatacac cggcggcaac cctataagcg gatgaaaaaa acagttaaac cggtcgtgga
      841 tcacttcccg ggcaggcacc gtcaccgcct cagtgatccc gcagttccgg tccggcgtga
      901 tgcggtagaa cacctcgccg tcatccgcca ccagaggttc aacccggttc cagtccagaa
      961 tacgcagttc tttgatctgc ccccgggcat tacggatttt cagcaccacc gtattgccgt
     1021 gacgcagttt ggcgttcagc cacagttcaa aaaactggat gcggttctgc tgggcgttgg
     1081 gacgacgaca gaggcgggca atatcccccc ggcgcgtttc cctgcgtatc ccctgcgcat
     1141 ccgtctgcat aagacgcagc cgcattttgg cgatatcctg ggatatcagc gaaatacatg
     1201 caaacaccgc atgaaaggag aggacggctt caggatcggc tttcac
//
LOCUS       DAZFVO020000375         1242 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFVO020000375 DAZFVO020000000
VERSION     DAZFVO020000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.382"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..353
                     /locus_tag="ACHKNB_RS26245"
                     /old_locus_tag="pgaptmp_005249"
     CDS             <1..353
                     /locus_tag="ACHKNB_RS26245"
                     /old_locus_tag="pgaptmp_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198560.1"
                     /translation="SIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQA
                     DKTDIRKTAEEKVKTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRG
                     MTNAEIDALLEKLG"
     gene            377..>1242
                     /locus_tag="ACHKNB_RS26250"
                     /old_locus_tag="pgaptmp_005250"
     CDS             377..>1242
                     /locus_tag="ACHKNB_RS26250"
                     /old_locus_tag="pgaptmp_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGU4198561.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECA"
BASE COUNT          321 a          301 c          338 g          282 t
ORIGIN      
        1 tatccatcga cgggaagctc cagaatgacc ccgcatggaa agacaaaacg ctcactgaac
       61 gtttcgctga agtggcccgt cgtacgcagg ttgctttcgg tgaagtcagt gagtcgtctg
      121 ctgacaacca ggcagacaaa acggatatcc ggaaaacggc ggaagagaaa gtgaagaccg
      181 ctgaacagga gcaggcagta cctgcttccc cgtcagattt aggcaccacg gcctccgtcg
      241 gaatcggtga taattttgaa cggttacttg gtgcttctca ttcagaggct gaggcgatta
      301 tgcgtggtat gacgaatgct gaaatagacg cacttctgga gaagctcggg taacttactg
      361 aaggagtact gaagtaatga cgactgtaac atcagcccag gcgaataagc tgtatcaggt
      421 ggcgcttttt actgctgcca accgcaaccg ctcgatggtt aatatcctta ctgaacagca
      481 ggaagcgcca aaagcggttt cgccggacaa gaaaagcacg aagcagacca gcgcaggtgc
      541 gccggttgtc cgtatcacag accttaacaa acaggccggt gatgaagtga ccttcagcat
      601 catgcacaaa ctctcaaaac gtccgacgat gggagatgag cgtgttgaag gtcgtggtga
      661 ggatctcagc catgctgact tctccctgaa aatcaatcag ggacgtcacc tggtggatgc
      721 aggcggacgt atgagtcagc agcgcacgaa gtttaacctg gcatcctctg ccagaacgct
      781 tctggggacg tactttaatg acctgcagga ccagtgtgcg atagtgcatc ttgctggagc
      841 tcgtggtgat tttgttgctg acgacactat tctgccgaca gcggagcacc ctgaattcaa
      901 aaaaatcatg atcaacgatg tactgcctcc gacacatgac cgtcactttt ttggcggtga
      961 tgcgacaagc tttgagcaga ttgaagcggc agatattttt tctattggcc tggtggacaa
     1021 tctctccctg ttcattgacg aaatggcgca tccattacag ccggttcgtc tgtccggtga
     1081 tgaacttcac ggagaagatc catattacgt cctgtacgtc acgccgcgtc agtggaatga
     1141 ctggtacacc tcgacgtccg gtaaggactg gaaccagatg atggttcgtg ccgtgaaccg
     1201 tgcaaaaggt tttaatcatc cgctgttcaa aggtgaatgt gc
//
LOCUS       DAZFVO020000376         1224 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFVO020000376 DAZFVO020000000
VERSION     DAZFVO020000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.383"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..448)
                     /locus_tag="ACHKNB_RS26255"
                     /old_locus_tag="pgaptmp_005251"
     CDS             complement(<1..448)
                     /locus_tag="ACHKNB_RS26255"
                     /old_locus_tag="pgaptmp_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198562.1"
                     /translation="MAFSDEQRPEAQLGNQNRSSLNIKQPGENYWQDFFSNPQNAIDH
                     STSFSLGDVLPTMGKGVAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPGV
                     ATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGA"
     gene            complement(533..>1224)
                     /locus_tag="ACHKNB_RS26260"
                     /old_locus_tag="pgaptmp_005252"
     CDS             complement(533..>1224)
                     /locus_tag="ACHKNB_RS26260"
                     /old_locus_tag="pgaptmp_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198563.1"
                     /translation="SGVFAPELQQRIGMPDSTGEKTIKEARIGSIVPAQQEGYVLIGL
                     DLTYSDGSTAHKPVTEYGSAHPDDQNVLAIPVDKAIALVRDRSKFAEISKNYGYFMPK
                     QQGLSLKELQKEASKVVAQAIKDGRDAQAANDEFLAAYDSLPHQQKVQQRKTQQLLQK
                     WQPWAAGDPVKQEFVKEAAANAPALLEPGREKEVEIYYQNHLRDKKAEHEKALDYSAS
                     AAAEKIKGWMK"
BASE COUNT          270 a          356 c          273 g          325 t
ORIGIN      
        1 cagcaccatc actgacagcc ttaccggttg ccttcagacc tttaccgaca gtatcagtaa
       61 ccgcgttttt gccatcaggc agggtatcga tgacctcatc agcccccctg ctgccaccgg
      121 caaaaatatc ctgcacagtt gcgacacccg gcagccccat acggctgaac tcatttaaaa
      181 tacgcgcccc tgtttttacc gggctttgaa tcatcgcatc accgagtcca cgggccattt
      241 cccctgttcc ccggacggac tgggcaacac ctttacccat tgttggcaat acatcaccga
      301 ggctgaacga tgtgctgtga tcaatggcat tctgcgggtt gctgaaaaaa tcctgccagt
      361 aattttctcc cggctgctta atgttcaggc tgctgcggtt ctggttaccc aattgcgctt
      421 cagggcgctg ctcatctgaa aaagccatac agactccagt aaaaaaagcc cgacacgatg
      481 gtcgggcgta agtgaacgat attaacgggg agcgcgttac tgccgcatat ctttacttca
      541 tccagccttt gatcttctcg gcagctgcgg aagcgctata atccagcgcc ttttcatgct
      601 ctgccttttt atcccggagg tgattctggt aataaatttc cacctccttt tcacgaccag
      661 gttccagcaa agcaggcgca ttggcggcag cctcttttac aaactcctgt ttaaccggat
      721 cgcccgccgc ccacggctgc cacttctgta aaagctgttg cgttttccgt tgctgaactt
      781 tctgttgatg cggcagtgaa tcatacgctg ctaaaaactc atcattcgca gcctgagcat
      841 cacggccatc cttgatcgcc tgagccacta ctttgctggc ttccttctga agctctttca
      901 gagaaagtcc ctgctgcttc ggcatgaaat aaccataatt tttcgatatc tctgcaaatt
      961 tgctgcgatc cctgacaaga gcgatagcct tatcaacggg tatcgccagc acgttttgat
     1021 catcagggtg cgcactgcca tattctgtta caggtttatg cgcagtggag ccatcgctgt
     1081 atgtgagatc aaggccaata agtacgtacc cttcctgctg cgccggtacg atactgccaa
     1141 tcctggcctc ttttattgtc ttttccccgg ttgaatcagg catgccaata cgctgttgta
     1201 gttccggggc aaacacgccg gaaa
//
LOCUS       DAZFVO020000377         1214 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFVO020000377 DAZFVO020000000
VERSION     DAZFVO020000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.384"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..442)
                     /locus_tag="ACHKNB_RS26265"
                     /old_locus_tag="pgaptmp_005253"
     CDS             complement(<1..442)
                     /locus_tag="ACHKNB_RS26265"
                     /old_locus_tag="pgaptmp_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HGU4198564.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDK"
     gene            complement(1077..>1214)
                     /locus_tag="ACHKNB_RS26270"
                     /old_locus_tag="pgaptmp_005254"
     CDS             complement(1077..>1214)
                     /locus_tag="ACHKNB_RS26270"
                     /old_locus_tag="pgaptmp_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HGU4198565.1"
                     /translation="EYYNLNRAIYWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKEN
                     P"
BASE COUNT          355 a          247 c          195 g          417 t
ORIGIN      
        1 ttttatcatc actacaaata ttaactgaat ttatatcaat gattttttta ttcttatctt
       61 ttattttatt tactgtttca atgcaatcag cggcaagttg gagtggattt agatttttca
      121 gatatggtct tatgatcttt cccttactta ataaatagca tgtataggat tcggaaagtc
      181 cagcatctat tttgtacctc attatattag ataaggatgt gagaaattcc ttgttatcat
      241 tcgcagccat atatgccagt ccgagttcag cctctgcggc cttacaggca aaatagctta
      301 gtgaacacgc ttttcgtccc tctttattac tggctgatat tagtgcccat aatattgctt
      361 tgtctgccaa atctaactct ctctgaaatt gtcttgcagt aactgcactc gcagaaatag
      421 agaaaaaaat acaattgtta ataaccttgc catatttttt agtccgtaac tgttaatgtg
      481 ctatctggct ctgaaaatat taactcattt aatttttgca tgtcattaga taaaccagtt
      541 atacaaccct cggaacagaa tcgaggtcct aaagacggac caaaagcgtt taaacaacta
      601 tgtattgaaa atccattgcg attataagtt cctgtattaa ctgacggtac taatcgtatg
      661 ccgtcaggtt ttctattgct tgctcccggg tcattaacat tccaactcca taggccttga
      721 ggtattggcc atctatttat aaatgtgtac aacggggatt atttttacat tgcatattat
      781 taccagaagc gactggaata ctaagcacat cataaaaatt atcatcacat ggtatatctg
      841 aaacacacga aagcatacct aaagttatac tgtataaaca ggtggctaat cccaagggga
      901 tcgattccag aaatcgggtt caacggatac tgataaaaat tccacctacc ttccagccca
      961 atcggagcct gagtgttata ccgcccctgc tgcggttaat gttaccgata atagttatag
     1021 tacagccctg attcatcatc atactactgc tgcggcagtc tgatgaacta ttgcttttac
     1081 ggattctcct tattcttcct tctatccatt cgctttagct ttttgagctc ttgaagtttg
     1141 ctctttgtat caatattacc gttatttaca gcgaattcca tccaataaat agctctattc
     1201 aaattataat attc
//
LOCUS       DAZFVO020000378         1206 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFVO020000378 DAZFVO020000000
VERSION     DAZFVO020000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.385"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            48..833
                     /locus_tag="ACHKNB_RS26275"
                     /old_locus_tag="pgaptmp_005255"
     CDS             48..833
                     /locus_tag="ACHKNB_RS26275"
                     /old_locus_tag="pgaptmp_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGU4198566.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(970..>1206)
                     /gene="ykfM"
                     /locus_tag="ACHKNB_RS26280"
                     /old_locus_tag="pgaptmp_005256"
     CDS             complement(970..>1206)
                     /gene="ykfM"
                     /locus_tag="ACHKNB_RS26280"
                     /old_locus_tag="pgaptmp_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HGU4198567.1"
                     /translation="KTKVVNFATDITIQLTLIFLLIAIVIAPLIAPFVTGYVNANYHP
                     CGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
BASE COUNT          395 a          227 c          250 g          334 t
ORIGIN      
        1 aatgctaaat attctaatca ttatgacagg cgagggagtg tccaattatg aattcaaaaa
       61 agctttgttg catatgtgtg ttattctcgc tgcttgcagg atgtgcctct gaatcttcta
      121 ttgatgaaaa gaagaaaaaa gctcaagtca cacaaagtaa tattaataaa aacactcccc
      181 agcaactgac agacaaagat ttattcggta atgaaaccac tctggccgta tccgaagagg
      241 atattcaagc tgcgcttgat ggagatgagt tccgcgttcc ccttaattct cctgtaatac
      301 ttgttcaatc cggcaaccgc gcaccggaaa ccattatgca ggaggagatg cgaaaatatt
      361 atactgtttc cacattctct ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa
      421 acaaagataa aaatgagaac gaggatgttg ccagtgctga gaatatgaac tggatgcagg
      481 cactgcgttt tgtggctgca aaaggacatc agaaagcgat tattgtttac caggatacat
      541 tgcagacagg aaaatatgac tctgcgctga aatcaacagt atggtccgac tataaaaatg
      601 aaaaactcac cgacgctatc tccctgcgct acctggtacg tttcacgctg gtggatgtgg
      661 caacaggtga gtgggctacc tggtcgccgg tgaattatga atataaagtg cttccaccat
      721 tgcccgacaa gaatgaagcc agtacgactg atatgacaga gcagcaaatc atgcaactta
      781 agcaaaagac ctataaagcg atggtaaaag atttggttaa ccgctatcaa taataaatta
      841 tatctgccgc caggaatttc tggcggcaat aatacaaaat tctttgcata agaaattttt
      901 cttgacaaag acagtgcaag aaaggacatg gaatccggag ttaaaaactg tttgttttag
      961 aatatttaat tatttaactg ctgaatcttc cttgcgagaa atatattcat tattacattt
     1021 catgccattt ttaatataga ttgctcctgg gaaaatcccc gtattattac cgcaggggtg
     1081 ataattagca ttgacatatc ctgtgacaaa aggagctatt aaaggtgcta ttacgatagc
     1141 tattagtaaa aatataagag ttagctgtat tgttatgtct gtggcgaaat tgactacctt
     1201 cgtttt
//
LOCUS       DAZFVO020000379         1190 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFVO020000379 DAZFVO020000000
VERSION     DAZFVO020000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.386"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..426)
                     /locus_tag="ACHKNB_RS26285"
                     /old_locus_tag="pgaptmp_005257"
     CDS             complement(<1..426)
                     /locus_tag="ACHKNB_RS26285"
                     /old_locus_tag="pgaptmp_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198568.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKAT"
     gene            complement(496..>1190)
                     /locus_tag="ACHKNB_RS26290"
                     /old_locus_tag="pgaptmp_005258"
     CDS             complement(496..>1190)
                     /locus_tag="ACHKNB_RS26290"
                     /old_locus_tag="pgaptmp_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198569.1"
                     /translation="SGVFAPELQQRIGMPDSTGEKTIKEARIGSIVPAQQEGYVLIGL
                     DLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRDRSKFAEISKNYGYFMPK
                     QQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQPHQQKIQQQKLQQQVIN
                     WAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRIQKARGEQARDESASSAS
                     QFIRGLKQNYAQ"
BASE COUNT          266 a          331 c          272 g          321 t
ORIGIN      
        1 ggttgccttc agacctttac cgacagtatc agtaaccgcg tttttgccat caggcagggt
       61 atcgatgacc tcatcagccc ccctgctgcc accggcaaaa atatcctgca cagttgcgac
      121 acccggcagc cccatacggc tgaactcatt taaaatacgc gcccctgttt ttaccgggct
      181 ctgaatcatc gcatcaccga gtccacgggc catttcccct gttccccgga cggactgggc
      241 gaaaccttta cccattgttg gcaatacatc gcccaggctg aacgacgtac tgttatcctt
      301 ccagcgattc ggatcagaga aaaatgcttc atagctgtca gtttcgccgg gttgctgaat
      361 gttcaggctg ttacgattct ggttaccgag ttgcgcctca ggacgctgtt cctctgaata
      421 ggccatacag actccataaa aaaacccggc acaatggccg ggcatcagga aatggtatta
      481 atggaatatc gtgaattact gggcgtaatt ctgtttcagt ccacggataa actgagatgc
      541 agatgaagca ctttcatctc tggcctgttc ccccctggcc ttttgaatac ggagaaaatt
      601 cgcatacccg ttctccagca atttctgatt ctgaggttcc agcatttcag gctgacgggc
      661 cgctacattt ctggcaaatg acagcttatc aggatcatct cccgcccagt tgataacctg
      721 ttgctgaagt ttttgttgct gaattttctg ttgatgcggt tgtgaaccag tcgcagcata
      781 atattcatcc accgcagcct gagcattacc gccattcttg atcgcgtccg ccgctacgtt
      841 gctggccccc ttctgaagct ctttcagaga aagtccctgc tgcttcggca taaaataacc
      901 ataatttttc gatatctctg caaatttgct gcgatccctg acctgagcga tagccttatc
      961 aacgggtatc gccagcacgg tttgatcatc agggtgcgca ctgccgtatt ctgttacagg
     1021 tttatgcgcg gtggagccat cgctgtatgt gagatcaagg ccaataagta cgtacccttc
     1081 ctgctgcgcc ggtacgatac tgccaatcct ggcctctttt atcgtttttt ccccggttga
     1141 atcaggcatg ccaatacgct gttgtagttc cggggcaaac acgccggaaa
//
LOCUS       DAZFVO020000380         1173 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFVO020000380 DAZFVO020000000
VERSION     DAZFVO020000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.387"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..264
                     /locus_tag="ACHKNB_RS26295"
                     /old_locus_tag="pgaptmp_005259"
     CDS             <1..264
                     /locus_tag="ACHKNB_RS26295"
                     /old_locus_tag="pgaptmp_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein"
                     /protein_id="HGU4198570.1"
                     /translation="SQQLTSMLSVQQQRDRYMARQLLSAPVPSLLIAGGYHASKSMGV
                     PLHMEDLAAGTHPVVLMLAEKGMNITVDHADYVWFVAPDTTKR"
     gene            272..1048
                     /locus_tag="ACHKNB_RS26300"
                     /old_locus_tag="pgaptmp_005260"
     CDS             272..1048
                     /locus_tag="ACHKNB_RS26300"
                     /old_locus_tag="pgaptmp_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HGU4198571.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          343 a          279 c          261 g          290 t
ORIGIN      
        1 agccagcaac tgacgtcaat gctctctgtt cagcagcagc gggatcgcta catggcccgc
       61 cagcttctga gtgccccggt accgtcgttg ttgattgcag gtggttatca cgcgtcaaaa
      121 agcatgggcg taccgttaca catggaagat cttgccgccg gcacccatcc agtggtgttg
      181 atgcttgcag aaaaaggaat gaacatcaca gtagatcatg ctgactacgt gtggtttgta
      241 gcgccagata caacaaaaag gtaaaccatg gatgatgaat attctccact tcccacagtc
      301 ggtaaaatgg agcagctggt ttatctgttc tctactgctt catggactga ttttccttgc
      361 gtttatctgg cgtttttccg aagttcagcc cgctatgtct cctgcaccgg cgatcatgct
      421 gcaatgggca gaaattatag aggctccttc ttctccactt tcgcttccgg tgggaatcgc
      481 tcaacaggag tccgcagtga cagaagaaaa gcaacaaact gaggacagac aacaacgtcc
      541 tgtcactgaa gacagtgatg caaccattga aataaccagg aagaaaaagt catccgatgg
      601 agaaaagaga aagacacgtc cacctcgtaa gataaaggct caaaccagcg acagcaaccc
      661 cacagccgtt tccagtaatg ctgcacctca ggctctggtc gaatcgtccc gtattgctgc
      721 gccttttaac agtgactcaa caaagcgaga taattctgag gcatcttggg aaagcagagt
      781 aaaaggccac cttaaccgtt ataaacgtta ccctggcgat gccagaaaac gtgcacgaac
      841 cggcacagcc gtcgtgagtt ttaccgtaaa cacggaaggc actattgtca gtagtttctt
      901 ggaaatctct tcaggaaccc tttctcttga ccgcgaagct attgctgttt tagaacgcgc
      961 tcaaccactt cccaaaccac cgcctgaaat attggagggg ggattattta aagtaaaaat
     1021 gccgattacc tttaagttga aggagtaaag ggataattgg ccgttcaagt tcatttaact
     1081 gaaatttaat tttcattttt atgctgaaca ttttatcaaa cttttccact gtcgaaaaaa
     1141 tcaaaaagac agtagatcat aattaataaa caa
//
LOCUS       DAZFVO020000381         1170 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFVO020000381 DAZFVO020000000
VERSION     DAZFVO020000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.388"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            241..423
                     /locus_tag="ACHKNB_RS26305"
                     /old_locus_tag="pgaptmp_005261"
     CDS             241..423
                     /locus_tag="ACHKNB_RS26305"
                     /old_locus_tag="pgaptmp_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HGU4198572.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            347..733
                     /locus_tag="ACHKNB_RS26310"
                     /old_locus_tag="pgaptmp_005262"
     CDS             347..733
                     /locus_tag="ACHKNB_RS26310"
                     /old_locus_tag="pgaptmp_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HGU4198573.1"
                     /translation="MIKGPLRKSAPVSQPEANPKSPLLLNNPFQRENPMSEVKPLVTA
                     DAVKDALRSEDVRGALKQKVRQTFEAQIEADVDAILDTILGEQPGEQPEPEPQPEDIT
                     SENGDIQPESPVVDATEAQPEPGTML"
     gene            809..1024
                     /locus_tag="ACHKNB_RS26315"
                     /old_locus_tag="pgaptmp_005263"
     CDS             809..1024
                     /locus_tag="ACHKNB_RS26315"
                     /old_locus_tag="pgaptmp_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HGU4198574.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGD"
BASE COUNT          336 a          255 c          332 g          247 t
ORIGIN      
        1 caactgacag acatatcaaa ggcggcgaca tatacgttgc tgattgacag tgtgaaggtg
       61 gaagtgaaca acgcggatgc cgcggcatga taaaaaaggc cgccagcaat gaaggaaagg
      121 gatgaacttt gtccactaca tgaatgctgg cggctggtaa aacgaccatg atgacaccga
      181 agtctgcggt gtttttctac aaaaataagt aaatcatgtc aactatcacg gtgagaaacc
      241 atgacatttc tgaaccagtt aatgctgtac ttctgtacgg tggtctgtgt gctgtatctt
      301 ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca ggcagattga taaaagggcc
      361 gctgagaaaa tcagcgccag tcagtcagcc ggaagcaaac ccgaagagcc cattactcct
      421 taacaacccc tttcaacgag aaaaccctat gtcagaagta aaacccctgg tcactgctga
      481 tgcagtgaaa gacgcgctgc gctcagaaga tgtgcgtgga gcactgaaac agaaagtccg
      541 ccagacattt gaagcgcaga tagaagcaga tgtggatgcg attctggata cgatactggg
      601 agagcagcca ggcgaacagc cagagccgga accgcaaccg gaagatatca cctcagaaaa
      661 cggcgatatt caaccggaat caccggtggt tgatgcgact gaagcacaac ccgaaccggg
      721 caccatgctg taagggtgag tcagggccat cagtaaagta ctgctggcct ttttcatgtt
      781 gtgagcttcc gtgtgcggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt
      841 gtcatacacc acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt
      901 ttccccgtct cagtgggcgg caataggcgt gctggggagt ctgctgtttg ggctgctgac
      961 atatctgacg aacctgtatt tcaagattaa agaagaccgg cgtaaggcgg cacggggaga
     1021 ttaggtgatg aaccatgaag aaatgaatca gcgcttcagt cgtctggaaa atgaaattgc
     1081 tgaactgaat aaaaaactgt cgacgctgat gccttctgaa gatgaaaaaa aacgccgcga
     1141 tgagcagttt gctgcgtttg acgattattg
//
LOCUS       DAZFVO020000382         1163 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFVO020000382 DAZFVO020000000
VERSION     DAZFVO020000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.389"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            135..242
                     /gene="yniD"
                     /locus_tag="ACHKNB_RS26320"
                     /old_locus_tag="pgaptmp_005264"
     CDS             135..242
                     /gene="yniD"
                     /locus_tag="ACHKNB_RS26320"
                     /old_locus_tag="pgaptmp_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HGU4198575.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(295..1053)
                     /locus_tag="ACHKNB_RS26325"
                     /old_locus_tag="pgaptmp_005265"
     CDS             complement(295..1053)
                     /locus_tag="ACHKNB_RS26325"
                     /old_locus_tag="pgaptmp_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HGU4198576.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          327 a          281 c          274 g          281 t
ORIGIN      
        1 ttttttcata aaacgcagaa acagtgatca ggtgtacaac gccacccgac ggctgtaata
       61 acaaaaaatc ccttgatgcc tgtccctttt gttacactcc gttatcacgc acaagagata
      121 tgcaggacac tggtatgccg actaaacgct ttgataaaaa acactggaag atggtggtgg
      181 tgctactggc aatctgtggc gctatgttgt tgctacgttg ggcagcaatg atttggggct
      241 gagcagtgac aaacgaagac agccagaccg cataacacgg tccggcagat aattttacat
      301 gctgtagccc agcgataagg ttgtccgacg atcggtatgt tccggtgcag actctggagg
      361 ttcagagttc catgtgacgt tgtaagccac tttcagtcca aaatgttcat taatagcaac
      421 atttaatgcg ctttcggagt tcagcgttgt gtcttccgcg ccaaagacgg aaacaccctg
      481 cgtaaattta gcgttgtcag tcaactgcca tgcataagca ccggaggcat agcccagcgg
      541 ctgagtttca ctggcattgt cggtatattt gtcgtaacgc acacctggac cgaattcaaa
      601 gcggaaactg tgtaccgggc cattcaaaaa ctgacgacca taacccgcgg tcaacacatc
      661 gcgctctcga tagccgttat aacggtctgt cagccagctt gcctgaccaa ataaatagtc
      721 ataatcagtt aaattaaaac ggctacgccc gcccgccgca tatttttctg aagaacgctc
      781 atcattagaa gaagtattac tggcgttccc ccacagcgac caggcagtgg tttgtccata
      841 ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc gtattgcctg attgtgcaag
      901 atatcctgcg ttcagattac cttcgaaagg ttttttagcg ctggcaggat cgtccatgac
      961 agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca aacatgcccc ccgccaacat
     1021 aacgatggca ggaactgtct tcaaaagctt catttatcaa gagtccgtac aacaaaaaaa
     1081 gagaccatcg cggtcccgga aactttctta aggatcaaag attagcgtcc ctggaaaggt
     1141 aacgaattat aaaaaggcgc gaa
//
LOCUS       DAZFVO020000383         1158 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFVO020000383 DAZFVO020000000
VERSION     DAZFVO020000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.390"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1158
                     /locus_tag="ACHKNB_RS26330"
                     /old_locus_tag="pgaptmp_005266"
     CDS             join(<1..274,274..>1158)
                     /locus_tag="ACHKNB_RS26330"
                     /old_locus_tag="pgaptmp_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4198577.1"
                     /translation="KTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGS
                     TTLESWVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVR
                     LAERFTIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQ
                     SRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNL
                     LERQFAVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSA
                     LRMAYETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFF
                     RSLKTEWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRY"
BASE COUNT          310 a          299 c          319 g          230 t
ORIGIN      
        1 aaaacaggtg acttaatgaa caagaaaacc aaacgaacct tcacccctga gttcaggctg
       61 gaatgtgcac agctgattgt tgataagggc tactcatatc gacaggccag tgaagcgatg
      121 aatgtcggtt caaccacgct tgagagctgg gtacgccagc tcaggcgaga gcgccagggt
      181 attacgccct ctgccacacc cattactcca gaccagcaac gtatccgcga gctggaaaag
      241 caagttcgcc gtctggagga acaaaatacg atattaaaaa aggctaccgc gctcttaatg
      301 tccgactcgc tgaacggttc acgatagccg ccagactaag tgacagccac acggttgtca
      361 gcctgtgttc tgctctggaa atacaccgca gcagttaccg gtactggcga aaacgacgcg
      421 atacggttaa tccggcacga gtcaggttgt gcagcgaaat acgccgggcg tggaaccaaa
      481 gtcggggctc tgcgggcgcg cgcactctgg ctgaaatgct gactcaaaac ggcgtcccga
      541 tgagccgtta ccgtgccgga cgtctgatga aatatctgaa cctgagcagt tgtcagcccg
      601 gaaaacatca gtataaaaat gcccgtcagg agcataccag cctgccgaat ctgcttgagc
      661 gtcagttcgc agtaccggag ccagaccggg tatggtgtgg agatattacg tatctctggg
      721 caggaaatcg ctggtgctat ctggcggttg ttatggatct ttttgcccgc agggttatcg
      781 gctggagtct gtcagcgcat gccgataccg cactgataag cagtgccctg cggatggcct
      841 atgagacgcg tggccaaccc cgggatgtcg tgttccatag cgaccaggga agccagtata
      901 caggccttaa atatcaacaa cttctctggc gttgcagaat aaatcaaagc gtcagtcggc
      961 ggggaaactg ctgggataac agccccatgg aacgcttctt ccgcagtctg aaaacagaat
     1021 gggtgccaac gaatggttac gcaggcaagg acgaggcccg gcagcaaatt aatgattaca
     1081 tattgaacta ctacaacagc gtcagacctc accattataa cggtgggctg acgccggaag
     1141 agtcagagaa cagatacc
//
LOCUS       DAZFVO020000384         1157 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFVO020000384 DAZFVO020000000
VERSION     DAZFVO020000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.391"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            55..591
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS26335"
                     /old_locus_tag="pgaptmp_005267"
     CDS             55..591
                     /gene="yfdR"
                     /locus_tag="ACHKNB_RS26335"
                     /old_locus_tag="pgaptmp_005267"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HGU4198578.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVMPLAPGHA
                     YGMFMERFNELSELRTYA"
BASE COUNT          354 a          255 c          249 g          299 t
ORIGIN      
        1 gcttcctttt agcagaattc aacgcggtgc agcgcatata aagtggagaa cgaaatgtca
       61 tttattaaaa ctttttccgg gaagcatttt tattatgaca ggataaataa agacgacatc
      121 gtgattaacg atatcgcagt ttccctttca aatatctgtc gctttgcagg acatctttca
      181 cacttctaca gtgtcgccca gcatgcggtg ctttgcagcc agctggtgcc gcaggaattt
      241 gcttttgaag cgttaatgca tgatgcaaca gaagcgtatt gccaggatat tcccgctcca
      301 ctgaaacgcc ttcttcctga ctataaacgg atggaagaaa aaatagacgc cgtaatccgt
      361 gagaaatacg ggttaccccc ggttatgagc acgcctgtga aatatgccga tctcatcatg
      421 ctggcaaccg aacgccgcga tctcgggctt gatgatggct ctttctggcc agtactggaa
      481 ggtatcccgg caacagagat gttcaacgtg atgccactgg caccgggcca tgcctacggg
      541 atgtttatgg aacgctttaa cgagttatcg gagttacgca catacgcatg agtctttcaa
      601 ctgaacaacc gctggagggg tagcaatgag caacttaatg acaacaaaac aagtcgccga
      661 attctgtggc gtttcaatat cgacggtgct tcgctggaac agcgtaaaca ggaggacagg
      721 ccagaaatac aggccagatt ttccagatcc tgatattaaa tcctgcccaa ataaatgggc
      781 atcacgcaaa atatacagat ttgctggagt tattgagtaa tgagagttag ccaagaggtg
      841 agatagtatc catctatggc acagaactaa acataatctg actatatgct ctgtgccaaa
      901 aacagatgtt atatcatgta ttgtatgctc atcctgcaaa atataaattc cgcagacact
      961 cctcacctct gaatattaat ccacaatgga tgcgacaaaa aagattttga gttggtatca
     1021 ggcgaaatat taataattat aataccccat gcacaatgcc atctgagtca tcagatcctg
     1081 gtctctatca atatttatct tgccatcaga aaaatccaac acaaagaaat gatgaaattt
     1141 cggcggaaca actttag
//
LOCUS       DAZFVO020000385         1142 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFVO020000385 DAZFVO020000000
VERSION     DAZFVO020000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.392"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..456
                     /locus_tag="ACHKNB_RS26340"
                     /old_locus_tag="pgaptmp_005268"
     CDS             <1..456
                     /locus_tag="ACHKNB_RS26340"
                     /old_locus_tag="pgaptmp_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HGU4198579.1"
                     /translation="RGDIHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFV
                     REDDWWRNGQNLYLDNLAENGFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELL
                     HHIPEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVVASACM"
     gene            453..1034
                     /locus_tag="ACHKNB_RS26345"
                     /old_locus_tag="pgaptmp_005269"
     CDS             453..1034
                     /locus_tag="ACHKNB_RS26345"
                     /old_locus_tag="pgaptmp_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HGU4198580.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          245 a          278 c          366 g          253 t
ORIGIN      
        1 cggggtgaca ttcaccggtt ccgctgtgtg ccgcacctga ccggacggcg ctttgaacac
       61 ggtgtgacgg actgttacac cctgttccgg gatgcttacc atctggcggg gataacgctg
      121 ccggattttg tgcgtgagga tgactggtgg cgcaacggtc agaacctgta cctggacaac
      181 ctggcggaaa acggctttta ccgggtgtct ccgtcccgtg cacaggcagg cgatattctg
      241 ctgtgctgct ttggcgcatc ggtgccgaat catgccgcca tttactgtgg caacggtgaa
      301 ctgcttcacc atatacctga acaactgagt aaacgggaga ggtattcaga gaaatggcaa
      361 cgacgaacgc attctgtctg gcgtcaccgc cactggtccg catctgcctt cacggggatt
      421 tacaacgatt tggtcgtcgc atcagcctgt atgtgaacac ggcagcggag gccatccgtg
      481 ccctgtcgct gcaggtgccg ggattccgct gtcagatgaa cgaaggctgg tatcagatac
      541 gtattgccgg tgaggatacc gcgccggagg cggtgtatgc ccgtcttcat gagccactga
      601 gcgggagggc cgtgattcat attgtaccga ggctggcagg ggccggggga aatggtgttt
      661 ttcaggtggt gctgggggct gcagccatcg tgggttcttt cttcaccgcc ggtgcaacga
      721 tggcgttgtg gggcgcagcc ctgagtgccg gagggctgac tgccaccacg atgctgttct
      781 cactgggtgc cagcatgata ctgggtggtg tggctcagat gctggccccg aaggcaaaga
      841 cgccggagta caaaagtacg gataacggta aacagaacac gtatttttcg tcactggaca
      901 acatgattgc ccagggtaac ccgatgccgg tgccttacgg tgaaatgctg gttggttcac
      961 gacggatatc ccaggacatc agcacccgtg atgagggcgg tgacgggaaa gtggtggtta
     1021 tcgggcgggg atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga
     1081 tgaagattaa ctttatggag ttatttttca ggcatcaaaa aagtaacgca gcgtcattat
     1141 tg
//
LOCUS       DAZFVO020000386         1134 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFVO020000386 DAZFVO020000000
VERSION     DAZFVO020000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.393"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            104..1078
                     /gene="cysB"
                     /locus_tag="ACHKNB_RS26350"
                     /old_locus_tag="pgaptmp_005270"
     CDS             104..1078
                     /gene="cysB"
                     /locus_tag="ACHKNB_RS26350"
                     /old_locus_tag="pgaptmp_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HGU4198581.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          295 a          268 c          270 g          301 t
ORIGIN      
        1 agtggttata gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga
       61 cgaaaaaacg atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact
      121 tcgctatatt gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact
      181 ttacacatca caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat
      241 tcaaattttt tcccgaagcg gcaagcacct gacgcaggta acgccagcag gacaagaaat
      301 aattcgtatc gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggaga
      361 gcacacctgg ccggataaag gctcgctgta tatcgccacc acgcataccc aggcacgcta
      421 cgcattacca aacgtcatca aaggctttat tgagcgttat cctcgcgttt ctttgcatat
      481 gcaccagggc tcgccgacac aaattgctga tgccgtctct aaaggcaatg ccgatttcgc
      541 tatcgccaca gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg
      601 gaatcgggct attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat
      661 tgaagaactg gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga
      721 actggatact gcctttaacc gcgcagggtt aacgccgcgt atcgttttca cggcaacgga
      781 tgctgacgtc attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat
      841 ggcggtggat ccggtcgccg atcccgacct tgtgcgcgtt gatgctcacg atatcttcag
      901 ccacagtaca accaaaattg gttttcgccg tagtactttt ttgcgcagtt atatgtatga
      961 tttcattcag cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt
     1021 gcgctccaat gaagaaattg aggtcatgtt taaagatata aaactgccgg aaaaataatt
     1081 tcttgcgtta ttttcggcac cttttatgta gcgaaggtgc cggaatatat tctc
//
LOCUS       DAZFVO020000387         1131 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFVO020000387 DAZFVO020000000
VERSION     DAZFVO020000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.394"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(89..295)
                     /locus_tag="ACHKNB_RS26355"
                     /old_locus_tag="pgaptmp_005271"
     CDS             complement(89..295)
                     /locus_tag="ACHKNB_RS26355"
                     /old_locus_tag="pgaptmp_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HGU4198582.1"
                     /translation="MERKAIEQILAKQPVMQVSVKIAAVSKYGDHQVEIRCKDTDRLI
                     WRAWDFEKDFKEDLERELLRLAPL"
     gene            complement(366..725)
                     /locus_tag="ACHKNB_RS26360"
                     /old_locus_tag="pgaptmp_005272"
     CDS             complement(366..725)
                     /locus_tag="ACHKNB_RS26360"
                     /old_locus_tag="pgaptmp_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019706033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198583.1"
                     /translation="MSKGNTLLINRIAVIGLAVVAAATQAAAPPVKFFYRDTVPVSIT
                     LDEPATGCSMAGVIVHPLDRPAAKVAWFSERICGKKRYPVSFISDEISAPDNVIHKGE
                     TFHVTPAQVNLLDAQSL"
     gene            complement(718..1020)
                     /gene="ccgD"
                     /locus_tag="ACHKNB_RS26365"
                     /old_locus_tag="pgaptmp_005273"
     CDS             complement(718..1020)
                     /gene="ccgD"
                     /locus_tag="ACHKNB_RS26365"
                     /old_locus_tag="pgaptmp_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CcgD"
                     /protein_id="HGU4198584.1"
                     /translation="MHFTNFLQRYFDIEIEHTFDPTIQGSNETGKDVTKIWIYEKGED
                     SEPLLILTEAWWYTETKTAGNWLIGNVYSTLEHRREIHENEFRKLVTAGKVISQHE"
BASE COUNT          265 a          297 c          260 g          309 t
ORIGIN      
        1 cggccggtta cgggggacac cgcaccgaca cggccagcca taaaaaaagc gcccatatgg
       61 gcgccatttt tcatagtagg gcagtgggtt aaagcggtgc aaggcgcaga agttcacgct
      121 ctaaatcttc cttaaaatcc ttctcaaaat cccaggcacg ccagataagc cggtctgtat
      181 ctttgcaacg gatttcaacc tgatgatcgc catatttgga aactgccgct attttcacgc
      241 taacctgcat taccggctgc ttagccagga tttgctctat agcttttctt tccattccca
      301 tgctccttta atactgccga taccggcgat tattggggtg gcggtattgc cacccgatga
      361 tttaactaga ggctttgcgc gtccaggaga ttgacctgag ccggggtaac gtgaaacgtt
      421 tcccctttat ggatcacgtt atccggggcg ctaatttcat cactgataaa gctaaccggg
      481 taacgttttt taccgcaaat ccgctcgcta aaccatgcca cttttgccgc tggccgatcc
      541 agtggatgaa caatcacacc ggccatgctg caacccgttg cgggttcgtc cagcgttatg
      601 cttaccggga ctgtatcccg gtaaaaaaac ttaaccggcg gcgcggctgc ctgcgttgcg
      661 gctgcgacaa ctgcaagccc gataaccgct atccgattaa taagcaatgt attcccctta
      721 ctcatgctga cttatcacct tgccagctgt taccagttta cgaaattcgt tttcatgaat
      781 ttcacgccta tgctcaagcg ttgaatacac gttgccgata agccagttac cagccgtttt
      841 tgtctcggta taccaccatg cttctgtcag aataagcagc ggttcgctat cctcgccctt
      901 ctcatagatc cagattttag tgacgtcttt acccgtttcg ttgctgccct gaatggtagg
      961 gtcaaaggta tgttcaatct ctatatcgaa gtaacgctga aggaagttag taaagtgcat
     1021 gacgactcct gtttgcgccg ataccggcaa tttagcgggt ggcggtgttg cctcccgatg
     1081 atttaattat cggtgattat gcctttaaag tcaatacagg tacggaatat a
//
LOCUS       DAZFVO020000388         1131 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFVO020000388 DAZFVO020000000
VERSION     DAZFVO020000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.395"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1131
                     /locus_tag="ACHKNB_RS26370"
                     /old_locus_tag="pgaptmp_005274"
     CDS             <1..>1131
                     /locus_tag="ACHKNB_RS26370"
                     /old_locus_tag="pgaptmp_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGU4198585.1"
                     /translation="LASKEAVAYFRAKGAHISWNWFETDADVHARSFTAAKVARLDVL
                     TTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGDAEEVQLGSPRRLA
                     LIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSALNGLVFRYDDPFW
                     NTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGVDKQTGEVREMPV
                     TTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREMVVQEMNNSPERQ
                     LAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLLAMSGKNVLHADS
                     MKHQNDGIALTPEDFGRLPAMLATPKAILWDKRHNNLMYIVES"
BASE COUNT          272 a          304 c          348 g          207 t
ORIGIN      
        1 cttgcctcaa aagaggcggt ggcatacttc cgcgccaaag gggcgcatat cagctggaac
       61 tggttcgaaa cagacgcgga tgttcatgcc cgttcattca cggcggcaaa agtggcacgc
      121 ctggacgtac tgaccacact acaggcggaa gtgcagcgtg ctgttgatga ggggatttca
      181 cagaaagcat ttatccgcac actgacaccc cgcctgcaaa agctgggatg gtggggaaaa
      241 cagattgtgg tggacagtgc cggtgacgcg gaagaagtgc agctgggcag tccccgccgt
      301 ctggcgctga tttacaacgt gaacacccgc gtggcttaca atgccgggcg ttacacgcag
      361 atgatgaaca acacggacac gcatccgttc tggcagtatg tggcagtgat ggacagccgc
      421 acccgcccgg cgcattccgc ccttaacggg ctggtattcc gctatgacga tccgttctgg
      481 aatacacact acccgcccaa tggctggaac tgtcgctgcc gtgtccggcc attgtctcag
      541 gcccgtctgg atgcgatggg gctgtctgtt tcatccggtc aggatcatct ctccacccgc
      601 aatgttgagg ctggcgtgga taagcagacc ggggaagtca gagaaatgcc ggtgaccaca
      661 tattcagatg gcaccagaac catgacaccg gatgtgggct ggtcatataa ccccggttcg
      721 gcggcgttcg gcacagacca ggcgctgatc cgtaaactga tcgaggtgaa aagtccggcg
      781 ctgcgggaaa tggtggtgca ggagatgaac aacagcccgg agcggcagct ggcgttccgc
      841 atctgggcaa aaaacatcat gaaaacccgg cgaggcggta acgatatccg cacgctgggc
      901 tttatgaccg aaagtattgc gcaggcggtg gaaagccgga cgggaacgcc accggcccgc
      961 ctgctggcga tgagcggtaa aaatgtgctc catgcggaca gcatgaaaca tcagaatgac
     1021 ggcattgccc tgacgccgga ggacttcggg cgattaccgg caatgctggc aacgccaaaa
     1081 gcgatactgt gggataaacg ccacaacaat ctgatgtata tcgtggaaag t
//
LOCUS       DAZFVO020000389         1121 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFVO020000389 DAZFVO020000000
VERSION     DAZFVO020000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.396"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..96
                     /locus_tag="ACHKNB_RS26375"
                     /old_locus_tag="pgaptmp_005275"
     CDS             <1..96
                     /locus_tag="ACHKNB_RS26375"
                     /old_locus_tag="pgaptmp_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4198586.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            196..1068
                     /locus_tag="ACHKNB_RS26380"
                     /old_locus_tag="pgaptmp_005276"
     CDS             196..1068
                     /locus_tag="ACHKNB_RS26380"
                     /old_locus_tag="pgaptmp_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198587.1"
                     /translation="MNKISIDSDNRRIIYREMNVSRLLTGLKHVDVNNTIRRIYNMCP
                     EAHVTASNIIFNNHSYPDFYPLKQTICKDIIRNTLLSWFPIFSNKKCSSDMVLMFRDG
                     EYATLSSLLFFNEQAEEWLNKGPEYWEYSLLVKNEITSLWCKELHKLPELEVRTISSP
                     GLFLLKNMDIDVSSYAIYSNYLEKMKTRHEAVLRFFSRIILLARTLERNSQLHWNRFN
                     YIDWKVGVVETARGWLVHMAKISDEGIVIDYKIISPTTYFTSEDGLLSNTLKKCQGDL
                     LNKYIVLLAPGAEV"
BASE COUNT          371 a          189 c          224 g          337 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa ccagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcatact ggtgttgccc ggtttgccag acaacaaaag agggggttct taaactggac
      181 tcaaaggagt gaatattgaa taaaataagt atagacagcg ataatcgtcg tatcatctac
      241 agggagatga atgtttcaag gttattgaca ggccttaaac atgttgatgt aaacaatacc
      301 atcagacgga tttataatat gtgtccagaa gcacatgtta ctgcgtctaa tattatattt
      361 aacaatcatt cttaccctga tttttacccg cttaaacaga caatatgtaa ggacattata
      421 agaaatactc tgctttcatg gttcccaata ttcagcaaca aaaaatgctc atctgatatg
      481 gtgcttatgt ttagagatgg agaatatgca accctttctt cattactatt tttcaatgaa
      541 caagcagaag aatggctgaa taaaggtcct gaatactggg agtactcttt actggtaaag
      601 aatgaaataa ccagtttatg gtgcaaggaa ctccataaac tacctgaact tgaagtaaga
      661 accatctcca gtccgggatt atttttactg aaaaatatgg atattgatgt ttcgtcgtat
      721 gctatatact ctaactatct ggagaaaatg aaaacaaggc atgaagctgt tttacgtttc
      781 ttttcaagaa ttatattact ggcccgaacc ctggagagaa acagtcaact acactggaac
      841 agattcaatt atatagactg gaaagttggt gtagttgaaa cagcaagagg atggcttgta
      901 catatggcta aaatatctga tgagggaatc gtgattgact ataaaatcat atcgcctaca
      961 acgtatttca cttcggaaga tggtctttta tctaatacat taaaaaaatg ccaaggagac
     1021 ctcctgaata aatatatagt attacttgcc cctggtgctg aagtttaatt tatttaaaag
     1081 gtatttgtat acacgagtta tctattgcac aatgtatgat a
//
LOCUS       DAZFVO020000390         1115 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFVO020000390 DAZFVO020000000
VERSION     DAZFVO020000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.397"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..597)
                     /locus_tag="ACHKNB_RS26385"
                     /old_locus_tag="pgaptmp_005277"
     CDS             complement(<1..597)
                     /locus_tag="ACHKNB_RS26385"
                     /old_locus_tag="pgaptmp_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HGU4198588.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGT"
     gene            complement(908..1012)
                     /gene="speFL"
                     /locus_tag="ACHKNB_RS26390"
                     /old_locus_tag="pgaptmp_005278"
     CDS             complement(908..1012)
                     /gene="speFL"
                     /locus_tag="ACHKNB_RS26390"
                     /old_locus_tag="pgaptmp_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HGU4198589.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(991..1113)
                     /locus_tag="ACHKNB_RS26395"
                     /old_locus_tag="pgaptmp_005279"
     CDS             complement(991..1113)
                     /locus_tag="ACHKNB_RS26395"
                     /old_locus_tag="pgaptmp_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HGU4198590.1"
                     /translation="MGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
BASE COUNT          341 a          239 c          238 g          297 t
ORIGIN      
        1 agtaccgttt aaaacgaaat aggttttatc ggcattaaag actttagccg catgctgttg
       61 tgcaatacat ggcgcacctt catgaatcag cagatcgccc attgctacgt cagcattaca
      121 caagtcagag cggaagagca tttcgccaaa gtattccacg aactgattgc ctgccggatg
      181 gcgacggaaa aactcacccc cctgatgacc agggcagtca aaagcactat tgccctgatt
      241 aacataatcg accagtgcgc ggaagaaggg cgggcgcagt tgcgtttcat aatggctggc
      301 agcggtttct aactgacgtc cataaaactc tttacgtgat tcgcaatgtt cgaagacacc
      361 agaaatacgt ggcagatatt ctgcgggtac acgttcttca ttttccgttg caataaaaac
      421 aggaatgtca tagccagtag cgtctatttc atcgagtttt ccacgttcaa catcactgac
      481 tgataaaata atggcggcaa cgtcaatata attactttcg tctatattca cgcatgcacg
      541 atgagtggaa aaacaatccg ggcaagaacg actaacggca atttttaatt ctgacatttt
      601 gttcatctct ttatttcagg taatagcagg tcctcaattt ctcaaaggag aaatcgatca
      661 cgccggaaaa aaagcaaagc tccaccgctg ataaataaat cagtagtttg ctttttgcag
      721 atgcaataac ccagaccagc ccggtaactg gctatactcg tcatacctca agttgcaagt
      781 gctgcgactc gaattattta gagtttaaaa gggttagcag tatatgagca ccgaggaata
      841 caggtattaa cctgtaatgc ttaaagtcag ggagacctgt acgttggggt gccctttagt
      901 cagaagacta gcgggcatta aagaaatgaa agtcaaaact attacgatgg gcaaacttca
      961 tgatatgggt tgtccgcctt atatgaggca ttaagcggtt attattttcc atttaccttt
     1021 acctcttgct gtgtcagttg aaggtagagt tattctatcc atgaaatggc tataatctgt
     1081 gaagaagatc aacaaaaaac gatcataccc caaat
//
LOCUS       DAZFVO020000391         1113 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFVO020000391 DAZFVO020000000
VERSION     DAZFVO020000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.398"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..517
                     /locus_tag="ACHKNB_RS26400"
                     /old_locus_tag="pgaptmp_005280"
     CDS             <1..517
                     /locus_tag="ACHKNB_RS26400"
                     /old_locus_tag="pgaptmp_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4198591.1"
                     /translation="NHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYC
                     LGALQVPFNQMKNPTDPEELANIFNKKHKEHMDDRFIHLIRSNAIASTPVYDNYFNNT
                     TAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKK
                     GVPGLSCFQPH"
     gene            779..>1113
                     /locus_tag="ACHKNB_RS26405"
                     /old_locus_tag="pgaptmp_005281"
     CDS             779..>1113
                     /locus_tag="ACHKNB_RS26405"
                     /old_locus_tag="pgaptmp_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198592.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVT"
BASE COUNT          392 a          180 c          207 g          334 t
ORIGIN      
        1 aaatcataaa ctctctgccg gtgatacgta taacttaatt aaaacgctcg atgtttgtaa
       61 ttatgactca gaaaggcaag ttttaaccag tcatcacgga ataataagag atgaagaaaa
      121 aaaatgttat tgtctcggtg ctttgcaggt tccatttaat cagatgaaaa atcctactga
      181 tcctgaggaa ttagccaata tttttaataa aaaacataaa gaacacatgg atgatcgttt
      241 tatccatcta attagaagca atgctatagc atccacacct gtatatgaca attattttaa
      301 taacactact gctttcagac caaaacctga agatatcttc aaatgtggac agacgctcaa
      361 aaaaactaaa cagaaatatg gccattatgg attaggggta gatcagcatc aaaaaattga
      421 taattatacc atggggctaa attcctggaa gatagctcct aacgaacgtg gtgataaaaa
      481 aggtgtccca gggcttagtt gttttcaacc acattaaaac tccatgagtg ctgtggtata
      541 tctttttaat cctgccagta tttcataggt tttcttaatc gattgtagag gcatgttctg
      601 atgacgcctc tacaatcttt agggactttc tctgctatga gataagaaat ttcatattat
      661 gctttaaggc tgaatgattt gtgcaaaata taattatttt tccatcgaac atgaggtgta
      721 tgtcataaga ttaaatgtta actatgcatt cctcgcggtg aattatatga ggtaaaagat
      781 gaaaattgga actgtggcag gcactaacga cagtacaacg acaatcgcaa caaatgatat
      841 ggtgcaagag catgttacta acttcactaa agaactgttt gggtatattg caaatggtat
      901 aggtgatgat atatcaagta ttgccaggac aatgctcggt gaagttgtgg aaaaaattga
      961 tgattggcaa atagaacgtt tccaacaatc tattcgagat gacaaaatat catttaccat
     1021 acaaacgaat cattcagaaa aatatagtat gctatcggac atgcgcgctc atattctacg
     1081 tagggataat aattatcagt ttattgtgac aat
//
LOCUS       DAZFVO020000392         1105 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFVO020000392 DAZFVO020000000
VERSION     DAZFVO020000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.399"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(867..>1105)
                     /locus_tag="ACHKNB_RS26410"
                     /old_locus_tag="pgaptmp_005282"
     CDS             complement(867..>1105)
                     /locus_tag="ACHKNB_RS26410"
                     /old_locus_tag="pgaptmp_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGU4198593.1"
                     /translation="KRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYAS
                     TPYFISVFREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          354 a          214 c          203 g          334 t
ORIGIN      
        1 ccggaaagag tgagtactgg atactcaccc tgaatctatt ttaaatgaca tcaggaatac
       61 ctgtccatac ttaagatttt tcctgtggaa aacaaacatt aatatatttt atgtatttcg
      121 catggtgatg atttatacac ctgcactgaa tttttctgta tttccagaac tgaatcgata
      181 aacagaattg gtaataatga aagttaataa cataaaaaag aggttaccgc cttataatga
      241 taagagttca ttatccattc catcccgaca aaacctgcct gtgtataaaa aataaggacc
      301 atgtcacaaa acattacatt ccggcaagtt atgacagaat tactgaaaac cgttatctgt
      361 agtaattgta gtttctgata tatgctcatc catccggaaa tacgacatgg tcctgactgg
      421 cgttcacttc aaagtgtaat ttattattca cacactgaga ctgaaaaacg gatggcgtgg
      481 atgagtacac agtctgaaga gtataaaagg aacagctccg gattgttacc cgggattact
      541 atagttcacc acacaaccgt aaacctggtt gtgtggtgaa acagtaacat attaagaata
      601 ttgtgttacc acctgttttc tcaggaaacg gattgcagga gaggtatact cattcatctt
      661 cccggagttt tgttaatcac tgcataatca aacggaccaa tacattcttt gctcgcatcc
      721 ttaaaaaggt cgccacaata tcatcagtca gtcaactcat taccatctgt tttccgggtc
      781 catagttact acagtgctcc ggagtgataa acagaaactg ataatgaagc ataagaatac
      841 ttttattatg cgctgactga catactttac ttaattgcga ttctgctctc aataaatccg
      901 gactgattaa tcatcatctg atgaggtgtt ataccaaaat attctctgaa aacgctgata
      961 aaatacggtg tgctggcata accgcaacgt attgcgattt gattcactga cagtttactg
     1021 gaaataagtt gtcgggcgtt atgcatgcgt atatccagca ggatttggga gaaagaaact
     1081 ttttcctcct gaagttttct tttca
//
LOCUS       DAZFVO020000393         1103 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFVO020000393 DAZFVO020000000
VERSION     DAZFVO020000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.400"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>1103)
                     /locus_tag="ACHKNB_RS26415"
                     /old_locus_tag="pgaptmp_005283"
     CDS             complement(<1..>1103)
                     /locus_tag="ACHKNB_RS26415"
                     /old_locus_tag="pgaptmp_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HGU4198594.1"
                     /translation="DGHRVDWMVNGLAGDALHKIGKGILVVAGSGENPGALNTGDGTV
                     ILAQKADAAGRVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDMAF
                     NRLAAADEGAVLTSRARPATVRLDFSQSGQKAVMWHGHFTGNLSVLNNTSSAVDFIMD
                     GGADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWES
                     RTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKG
                     TSAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRLTS
                     SALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDG"
BASE COUNT          240 a          372 c          247 g          244 t
ORIGIN      
        1 ccgtccacca aaagggttcc tctgtccagt gtcacaccac catctgtctc cagtcccccc
       61 ttcacatgta aacgcccgcc gtcactgaca gtaagtgcag aagaggtcag gcgggaattc
      121 ccttccagac gcacattctc tgaactcaca gctatccggc tgtccgatgc cgttatactg
      181 ccagtaaaat ggtcgcggac cgtcagggca gaatgacgca tattcacatt cccgcgaaat
      241 gtactgctac cggatgcacc gcccacggca gacgtcccct taaccggcgc actgctgctc
      301 cccgtgccgt ccttcatatc cagatatagc gagtcacttc ccagcaccat ggtggcatta
      361 tcagcatcaa tatctcccgt cagtgtcgca ttgcgggaaa gactgaatgc cgcccccttc
      421 agtttcagtt gaccgatgct gaacgttctg ctctcccagt ccttctgagc aaatgatacc
      481 ggctgcgtca gaacagaatt atcaccctgc tttcgcaatg cagtcgcaac cgcctcactg
      541 ctgacagcat gaacaaccgg atgcccctgg atatacagtc ccccgccctg ctgtgtaaaa
      601 ctgccggaca tatccgctcc gccatccatg ataaaatcca ccgcagatga cgtgttgttc
      661 aggacgctga ggttaccggt aaaatgacca tgccacatca cggccttctg ccctgactgg
      721 ctgaaatcca gacggactgt ggcaggacgc gcccggcttg tgagtactgc cccttcatca
      781 gccgcagcca ggcggttgaa ggccatgtca tttccgttga tatcaagcgt gcctccccgg
      841 tatccccagc gaatgcggtc cccttcaatc tgatgactgt cctgcagcac cacaactggt
      901 cgtccgctga caatactgac ttcactgaat gcccggaccc tgcctgcagc atcagccttc
      961 tgtgccagaa tgaccgttcc gtcccccgta ttcagggcgc cgggattttc accactgcca
     1021 gccaccacca gtattccctt gccaatttta tgaagtgcgt ctccagcaag tccgttcacc
     1081 atccagtcaa cacggtgccc gtc
//
LOCUS       DAZFVO020000394         1100 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFVO020000394 DAZFVO020000000
VERSION     DAZFVO020000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.401"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..841
                     /locus_tag="ACHKNB_RS26420"
                     /old_locus_tag="pgaptmp_005284"
     CDS             <1..841
                     /locus_tag="ACHKNB_RS26420"
                     /old_locus_tag="pgaptmp_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4198595.1"
                     /translation="AVLNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIGEYGATG
                     FINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTGSNDET
                     KAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGATGPAGP
                     RGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQIYAPQTDSTTWA
                     VDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEY
                     FPL"
     gene            883..>1100
                     /locus_tag="ACHKNB_RS26425"
                     /old_locus_tag="pgaptmp_005285"
     CDS             883..>1100
                     /locus_tag="ACHKNB_RS26425"
                     /old_locus_tag="pgaptmp_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198596.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVA"
BASE COUNT          292 a          230 c          366 g          212 t
ORIGIN      
        1 tgcggtactc aataaagtca tcacggatgt cggaccggga aaactcaatc tgggtggctg
       61 tgtcattgag gtgatgggga cgtataacgc agccatagtc cgggtcacca ttggcgaata
      121 cggtgcaacg gggtttatta atggcacggt ctgtacctgt acggtttacg gcgacacaca
      181 gtatttccac tggcgggtgg attacagcac aaaaaacaaa ccggatacgg tcagccagcg
      241 cgatgccagc acgacgcaga aaggtgtggt gcagttaagc agtgatactg gcagtaatga
      301 cgaaacaaag gcagccacgc cgaaggccgt gaaggcggca atggatgtgg caaatgaagc
      361 gaaaacaaag gcagaagagg ctgcagcagg aggtggtgtt cccggtccga aaggagagaa
      421 aggcgacccc ggagcacagg gtccgaaagg tgaaacggga gcaacaggcc ccgctggtcc
      481 gcggggagcg cagggaccga aaggggagaa aggcgacccc ggtcccagag gtgagcgtgg
      541 agaaaccggg ccgatggggc cgcccggggc atcagacggg aagagccggg ttgtgggtat
      601 caggctgggt aataaacaga tttatgcgcc gcaaacagac agcactacat gggcagtgga
      661 tttggatatt ggtgcaatga ttacggggat tggcggttac aacgacggaa acaaaaccct
      721 tattgacagg gtatcttaca ggccgcttca ggtgacattt gatggttctc agtggcgtac
      781 agtcagcgtg ggtgagtatg tgtcgtcagg agcttcgggg tttgagtatt ttccattata
      841 aagcgcatca tctttcagac atgtaaccgg gaggacttac agatgcacat aaagaatttc
      901 agacaataca cgccggaaaa tccggatgtg ccgggtgcga tgtacctgaa atcagaggat
      961 ggtcaggact ggtatgagtg tcagtcgtta ttttcagcag agacgctgaa ggtggtttat
     1021 aacagtgccg gagtcattac cggcatcagc agggtggcat cagttctgtg gccagtgggc
     1081 cagagtgtgg tggaggtggc
//
LOCUS       DAZFVO020000395         1099 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFVO020000395 DAZFVO020000000
VERSION     DAZFVO020000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.402"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..348)
                     /locus_tag="ACHKNB_RS26430"
                     /old_locus_tag="pgaptmp_005286"
     CDS             complement(<1..348)
                     /locus_tag="ACHKNB_RS26430"
                     /old_locus_tag="pgaptmp_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HGU4198597.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIE"
     gene            529..681
                     /locus_tag="ACHKNB_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /pseudo
     CDS             529..681
                     /locus_tag="ACHKNB_RS26435"
                     /old_locus_tag="pgaptmp_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(706..>1099)
                     /locus_tag="ACHKNB_RS26440"
                     /old_locus_tag="pgaptmp_005288"
     CDS             complement(706..>1099)
                     /locus_tag="ACHKNB_RS26440"
                     /old_locus_tag="pgaptmp_005288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198598.1"
                     /translation="VKMFKSSIRNIFYPSRLERLSRFQFIIYTLTSWSILITLNDINL
                     ALSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLS
                     VQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          354 a          186 c          208 g          351 t
ORIGIN      
        1 ttcaatcaga tcttcgataa gtgttttata ttgtccaaaa aaggaaagca tttttgctag
       61 ttctttcatt tctgcaaata aaatatcagt gcattcacgt tgtgatattt ttgttccgga
      121 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
      181 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      241 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      301 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      361 tggttatatt taagctcaat atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      421 aaaagaagta aacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      481 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      541 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggggcttc
      601 ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tgacaccgta tctgtacttt
      661 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      721 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      781 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      841 aaaaagtgtc aagcccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      901 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      961 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
     1021 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
     1081 tgatttgaac atttttaca
//
LOCUS       DAZFVO020000396         1096 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFVO020000396 DAZFVO020000000
VERSION     DAZFVO020000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.403"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..621
                     /locus_tag="ACHKNB_RS26445"
                     /old_locus_tag="pgaptmp_005289"
     CDS             <1..621
                     /locus_tag="ACHKNB_RS26445"
                     /old_locus_tag="pgaptmp_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198599.1"
                     /translation="NLKEIADAGAEAQRLARGYLGLGTLATKNSLGPADVNALAKDQN
                     LADLENKGTARNNLDVYSKSEGDNRYLRREQNGADIPDKGAFIDNVGLRETVNRAANA
                     LPSDGTAVAANRLANAHTINGVPFDGTQDITISSGTVTAIRLGSVTAHMPGTWESWDL
                     NLGGGNVLTGIKVQDVGKNTADNVGGVYYRPLQYLLNGAWVTAASI"
     gene            complement(653..>1096)
                     /locus_tag="ACHKNB_RS26450"
                     /old_locus_tag="pgaptmp_005290"
     CDS             complement(653..>1096)
                     /locus_tag="ACHKNB_RS26450"
                     /old_locus_tag="pgaptmp_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4198600.1"
                     /translation="DGKDFYESLPLFTKKYKLCITPDSGVICSISQDASALYPAGFSV
                     VEVDELPEGTDISGNWKFDNGIISRIPVNYARKLEAMRQSYLNQAYEKINDWRTELQL
                     GTISDEDRAALTQWMAYISQVKKMELPAIKTEAEFNAIKWPEQPQ"
BASE COUNT          270 a          253 c          292 g          281 t
ORIGIN      
        1 aacctgaaag aaattgctga tgccggtgcg gaggcccagc gtctggcccg tggttatctt
       61 ggtctgggga cgcttgccac aaaaaacagt cttggtcccg ctgacgttaa tgccctggcg
      121 aaggatcaga atctcgccga cctggagaat aagggaaccg cccgtaataa tctggatgtt
      181 tacagcaaaa gcgaaggtga taaccgttac ctgcgcaggg agcagaacgg cgcagacatt
      241 ccggataaag gggcttttat cgataacgtc ggtttacggg aaacggtaaa cagggcggcg
      301 aatgccctgc catcggacgg caccgccgtt gccgcgaaca ggctggcaaa tgcccacaca
      361 attaatggtg ttccctttga cgggacgcag gatatcacta tctcatcagg aacagtaacc
      421 gccataagac tggggtcggt gaccgcacat atgcccggaa catgggaaag ctgggatctg
      481 aatctggggg gcggcaatgt tcttaccgga attaaagtac aggatgtcgg taagaatacg
      541 gcagataacg tcggcggcgt gtattaccgg ccgttgcagt atctgttaaa tggagcatgg
      601 gtgacggctg caagtatata atgatattca gaaggccttc cggccttctt tattactgtg
      661 gctgctcagg ccatttaatg gcgttaaatt cagcttctgt tttaatggcg ggtagttcca
      721 ttttcttcac ctgactgatg tatgccatcc attgggtaag tgctgctctg tcttcatcgc
      781 tgatggtgcc cagttgtagc tccgttctcc agtcattaat tttttcatat gcctgattaa
      841 gatatgactg gcgcattgct tccagttttc ttgcgtagtt aaccggaata cgggagatta
      901 tgccattatc aaacttccag tttcctgaaa tatccgtgcc ttcagggagt tcatcgacct
      961 caaccacaga aaaccccgca ggatacagcg ccgaggcgtc ctgtgatatt gagcagataa
     1021 cgccgctgtc aggtgttatg caaagtttat attttttggt gaataatggc agggattcgt
     1081 agaaatcctt accatc
//
LOCUS       DAZFVO020000397         1096 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFVO020000397 DAZFVO020000000
VERSION     DAZFVO020000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.404"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            549..644
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS26455"
                     /old_locus_tag="pgaptmp_005291"
     CDS             549..644
                     /gene="dqlB"
                     /locus_tag="ACHKNB_RS26455"
                     /old_locus_tag="pgaptmp_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HGU4198601.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
BASE COUNT          298 a          262 c          273 g          263 t
ORIGIN      
        1 actgaaaaaa tgggatttat gaaaatgcca ccgtcggcat caagcagttg gtcagcgcaa
       61 ccggctgcat cgactgcaac aaaaaaactg aaagagaaag cccctcatat gagggagaaa
      121 aaaaggaagc gcagtcaacg agaggcgtaa aaaaacccga ctggtgaggt cgggttttaa
      181 tacggttccg gttctgagtt ggtggctctg accggaggaa gtcactctac acctaacgtt
      241 tacattttat gcactcagcc gcagtattgc aactaactgt ggttaaggca ccacatttag
      301 ttgtgtatcg tgggttttgt ataccttata atcatgaagt tagccgtgtg gtgtggcttc
      361 ttcctctgcc cttcccgttt atgcaggtat gaacgatttt aaagtcctgg ctgaacaaac
      421 aggtggtgct gtgtttagtg ctctggcagg aggaagtcac tctgcaccta acaatgcgag
      481 agaaccgttg tgacaggcag tgtaagagca agcaacggtg atttcctcca gttttactgg
      541 agaaacggat gattaatctg ttaatcatcg ttctcagggc tgtggtcgcc gttgcaaacg
      601 cgctgattgc aattctggaa ctgattcgcc agttcatcga ctgatgacag cggaaacgta
      661 actaagggca ggaagtgaac cgcttcctgc cctttaacgt actgtgacat ttttcaaaaa
      721 aactcaccaa tattctcctc ggcagaaatg acataactca ctgtaaaata aaaaatcatc
      781 taaacgctgg ctttgcatcg agcgcgttgg ccaacgcttt tatcagctgc gaccgcacca
      841 gataatttga acaagatttc ccgcattacc tggacataca ggttattttg taggctgcgc
      901 tcctccaagc cagttacctc ggttcaacga gcgccaactc cataaacctt tgaaaaaccg
      961 cccaccaagg cggttttttc gttcagccag acgacacatt tcagggcagc aggcacaggt
     1021 gatacagggc aggcggtgga ggccgcctgt cagcccgcgc gaaaacccct ggcaggctgg
     1081 cgcctgccgg aaggga
//
LOCUS       DAZFVO020000398         1061 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFVO020000398 DAZFVO020000000
VERSION     DAZFVO020000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.405"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            59..>1061
                     /locus_tag="ACHKNB_RS26460"
                     /old_locus_tag="pgaptmp_005292"
     CDS             59..>1061
                     /locus_tag="ACHKNB_RS26460"
                     /old_locus_tag="pgaptmp_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HGU4198602.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVSLP
                     "
BASE COUNT          332 a          170 c          251 g          308 t
ORIGIN      
        1 taaacatgaa aatagttacc gtgtcggtgt tttgattaat ttttaaaagg tggttacaat
       61 gaaagtgaaa aactttatat atcttccctt ctgcttgttt attggaacgt cggtagccgg
      121 agctttaccg gaaattcctg aaccatttaa atatggtgta ggagggatcg ataatggtaa
      181 aatatatata ggactgggaa gtctgggaaa taactggtat atgatagata caaaccagag
      241 tgaaaaaaaa tggactaaga tagcacaatg gccgacagtt cccagagagc aggcaacagc
      301 tactattatt gatggtaaga tctatgtatt tggaggaatt ggaaaagata aaagtggagt
      361 cataacctta caaaaagatg tctattccta tgatattgct aaagataaat gggaaaaatt
      421 gatgacccgt ccccctgtat ctcttgccgg tcatgtttct tttatccata atggtcatgc
      481 ggtatctact ggtggtgtaa atgagaatat ttttaatgga tacttttcag atgtcgagtt
      541 atcaaaaggg aattccgcgc tgacagagaa agtcaacagg gattatttta gtaaacctgt
      601 tgatgattat tttttaaata atcatatgat ttcttatgac ccgtcaaaaa accagtggaa
      661 gaacctggga acgactccat ttccgggaac tgctggttca tcggtgattt ttgcagaaca
      721 gcaaatttat atactgggag gggagagaaa accgggcctt cgttctgtta gatcctggac
      781 gggggagctt tcgcatgatc gaattaaatg gtctgaactt ccaccagtag cttcacccga
      841 gggggtgtca ggagcatatg cctctgttat tgatggaaat atctttctgg ccggtggtgc
      901 atatttccca ggggcggcag aaaagtatag caatggtgaa tactggtctc ataaaggact
      961 ggataaagcc tattcaaaag agatatatca gctaataaaa aatgactgga aaaaagttgg
     1021 ttctttgccg gaaggacttg cttatggtgt aagcctgccc t
//
LOCUS       DAZFVO020000399         1055 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFVO020000399 DAZFVO020000000
VERSION     DAZFVO020000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.406"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..554
                     /locus_tag="ACHKNB_RS26465"
                     /old_locus_tag="pgaptmp_005293"
     CDS             <1..554
                     /locus_tag="ACHKNB_RS26465"
                     /old_locus_tag="pgaptmp_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4198603.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYG
                     ARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVK
                     PNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPAT
                     AGIDDLGEILAKMKQRTSRGIRK"
     gene            551..>1055
                     /gene="ybfC"
                     /locus_tag="ACHKNB_RS26470"
                     /old_locus_tag="pgaptmp_005294"
     CDS             551..>1055
                     /gene="ybfC"
                     /locus_tag="ACHKNB_RS26470"
                     /old_locus_tag="pgaptmp_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HGU4198604.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPN"
BASE COUNT          349 a          199 c          232 g          275 t
ORIGIN      
        1 ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgc
       61 tgaatccggt tcagtatata gattcaatgg gactggcatc aaaatatgga cacttaaata
      121 atggcggata tggagcgaga cccaacaaac cgcctacgcc cgatccaagt aaattgccgg
      181 acatagcgaa acaattaaga ctgccatatc ctattgacca ggccagtagt gcgcctaatg
      241 ttttcaaaac attcttcaga gcattaagcc cttacgacta cacactgtat tgcaggaagt
      301 gggtaaaacc aaatctgact tgtacgccac aggatgattc ccagtatcca gggatggata
      361 caaagacagc aagtgattac ctgccacaga caaattggcc aacaactcaa ttaccaccag
      421 gatatacttg tgcagaaccc tatttattcc cagacattaa taaacccgat gggccagcaa
      481 cagcagggat agatgatttg ggtgaaattt tagctaagat gaaacagaga acatcgagag
      541 gaataagaaa atgaaaagag ttttgttctt tttgctgatg atatttgtta gttttggtgt
      601 tatagctgat tgcgaaatac aagctaaaga tcatgattgt tttactattt tcgctaaggg
      661 tactattttt tccgcgtttc ctgtcttaaa taataaagcc atgtggagat ggtatcagaa
      721 tgaggacatc ggtgagtatt attggcaaac agaactgggt acatgtaaaa ataataaatt
      781 cacaccaagc ggggcgagac tcttaattcg cgtcgggtca ctacgcctta atgaaaatca
      841 tgccatcaaa ggaacgttgc aggagttgat aaatacagca gaaaaaacgg cgtttctcgg
      901 cgatcggttt agaagttata taagagcggg tatctatcaa aaaaagagca gtgatccagt
      961 tcagttgttg gcagtccttg ataattcaat catggtaaaa tattttaaag atgagaaacc
     1021 aacttatgct cgaatgacag cacatctccc gaaca
//
LOCUS       DAZFVO020000400         1045 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFVO020000400 DAZFVO020000000
VERSION     DAZFVO020000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.407"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..331)
                     /locus_tag="ACHKNB_RS26475"
                     /old_locus_tag="pgaptmp_005295"
     CDS             complement(<1..331)
                     /locus_tag="ACHKNB_RS26475"
                     /old_locus_tag="pgaptmp_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HGU4198605.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQND"
     gene            339..764
                     /gene="tnpA"
                     /locus_tag="ACHKNB_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /pseudo
     CDS             339..764
                     /gene="tnpA"
                     /locus_tag="ACHKNB_RS26480"
                     /old_locus_tag="pgaptmp_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          286 a          255 c          224 g          280 t
ORIGIN      
        1 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt
       61 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa
      121 gccattgcgt ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag
      181 ggatgtattt attaccgacc tcatgattct cattctgacg tgccagagca cgattgaaaa
      241 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta
      301 actggaattt aaatgcctgt aatcgtttca caggccaaat gatactttgg tctgtgaaaa
      361 aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct
      421 ttgtcaccag ataccgacgt cagatttttg accatgacgc gacagaaaaa ctacgcactt
      481 acttttcaaa tgtatgtgct gattttgaag ctgaactggt tgaaatggat ggcgaaccag
      541 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
      601 gcctcaaagg cgtatcgggt aggtgactgc gacgagatcg accagatatt gcagtcaggt
      661 attactacaa aggcgttttg tggagtgctg gctattttgc cagtagctgc ggaggtgcgc
      721 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc
      781 gcgccttata tccctgccct gaaggacggg gttttacggc gcaccggata agcctggaga
      841 ttatctacac cagcatggac atcattgtac tatgccccgt acatgcccca tcacatcctc
      901 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
      961 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1021 tcaattacat taataaatcc aaaca
//
LOCUS       DAZFVO020000401         1033 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFVO020000401 DAZFVO020000000
VERSION     DAZFVO020000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.408"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..738
                     /locus_tag="ACHKNB_RS26485"
                     /old_locus_tag="pgaptmp_005297"
     CDS             <1..738
                     /locus_tag="ACHKNB_RS26485"
                     /old_locus_tag="pgaptmp_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3672 domain-containing protein"
                     /protein_id="HGU4198606.1"
                     /translation="VKAGVSITSPVIRSAVIQNGNFQVESDGSIKSGEHFSVSVNGDV
                     TASNAKISGHIDADSGTLNNVMIEENCIIKGTMSADRIIGDIVKGYSFEPHDGTLHIT
                     NHKYGEGYVIFKAEVSLADDVSFDRKLILLPFTFYSGTLTIEINGRVVIKADPRVANT
                     GTAGYFDRYDNPVYTRTNLGNTVSGVWDVAAGGGDTRITVTMRVEGGPGNMGDVEGRK
                     RELVRWRYDAFTLMVAKSTDSGFTRIS"
     gene            940..>1033
                     /locus_tag="ACHKNB_RS26490"
                     /old_locus_tag="pgaptmp_005298"
     CDS             940..>1033
                     /locus_tag="ACHKNB_RS26490"
                     /old_locus_tag="pgaptmp_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4198607.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRL"
BASE COUNT          284 a          209 c          290 g          250 t
ORIGIN      
        1 gtaaaggccg gggtaagtat cacttcacca gttatccgga gtgccgttat tcagaacgga
       61 aattttcagg ttgagtccga tggcagcata aaaagcgggg aacatttcag cgtttcagtg
      121 aatggagatg tgacggccag taatgcgaag ataagtggtc atattgatgc ggattccggc
      181 acgctgaata acgtgatgat tgaagaaaac tgcatcatta agggaacaat gagtgcggac
      241 agaatcatcg gagatatcgt caagggatat tccttcgaac ctcatgatgg cacgctccat
      301 attacgaacc acaagtatgg agagggttat gtcatcttta aggcggaggt atctcttgct
      361 gatgatgttt cgtttgatcg caagttgata ctccttccct tcactttcta ttcaggaaca
      421 ctgacgatag agattaacgg aagagttgtc attaaggcag acccaagagt tgctaatacg
      481 ggaacggctg gttattttga cagatacgac aatcctgttt acacgcgcac caacctcggg
      541 aacaccgttt caggagtatg ggatgtggcg gcaggagggg gagatacacg gattacagtg
      601 acaatgagag ttgaaggtgg tcctggcaat atgggggatg tggaagggag aaagagagaa
      661 ctggtgcgat ggagatatga cgcctttact ctcatggtgg cgaaatcgac agattccggt
      721 tttacccgga tatcgtgaca ggcagttgta atacggattg ccggaaggag acaaaaaccg
      781 tacagtatgc gcgggtgcct ttcggctgat ggctggaggg agaacctgaa ggcctgatat
      841 ggaaaggccc cgagtcaact ttaacgttaa cccgaggccc tgaccgtaca accttcacaa
      901 gtagtaggtt agcgcctctc cattcaggag acaagcgtta tgtcgcaaaa atcgctcatc
      961 accgtcacaa tttgcatgac ggttatcttc accatctgga tgttgcacgg ttcactgtgt
     1021 gagttccggc tga
//
LOCUS       DAZFVO020000402         1024 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFVO020000402 DAZFVO020000000
VERSION     DAZFVO020000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.409"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..>1024
                     /locus_tag="ACHKNB_RS26495"
                     /old_locus_tag="pgaptmp_005299"
     CDS             173..>1024
                     /locus_tag="ACHKNB_RS26495"
                     /old_locus_tag="pgaptmp_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HGU4198608.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRL"
BASE COUNT          246 a          256 c          295 g          227 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctg
//
LOCUS       DAZFVO020000403         1012 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFVO020000403 DAZFVO020000000
VERSION     DAZFVO020000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.410"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>1012
                     /locus_tag="ACHKNB_RS26500"
                     /old_locus_tag="pgaptmp_005300"
     CDS             <1..>1012
                     /locus_tag="ACHKNB_RS26500"
                     /old_locus_tag="pgaptmp_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HGU4198609.1"
                     /translation="DISQRLPGSQPGVNETTLNAIAYAQAGLSAQEHEHLAWIARQII
                     PTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGITEGVLLQRSDGVVYRITS
                     SLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFVTPQAGINQTATVTGTGITGGADVET
                     VPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRPEWPQAGSVGVTFVQDNN
                     PDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTVTVFKLTNKPVPFSIRIIPKTP
                     ENQAAVKQALTDLLYNESRPGGLVLASSFWRAVAGVKNLEDFEVRSPLTSVQAGDSEL
                     LTV"
BASE COUNT          201 a          271 c          344 g          196 t
ORIGIN      
        1 gatatcagcc agcgtcttcc cggttcgcag ccgggcgtga atgaaaccac cctgaatgcc
       61 attgcgtatg ctcaggcagg attgtctgca caggagcatg aacatctggc ctggattgca
      121 cgtcagatca tcccgacaga agccgatgaa gccgagttgc tgaagcactg cgctttctgg
      181 ggtgtcatgc gtaaacccgc ctcacggggt gacgggccgg ttcagctgat gctgaccacg
      241 gatgcgggga tcacggaagg tgtgcttctc cagcgcagtg acggcgtggt ttaccgcatc
      301 acctcctcac tgaccggtaa agccggtacg ctgaatgtca gtgtggaggc cgaaagtgcc
      361 ggtcgtgcgg gaaatgcccc ggcagggacg aaactgacgt ttgtcactcc gcaggcgggg
      421 atcaaccaga cggccacggt gaccggaaca gggatcaccg gtggtgcgga tgtggaaacg
      481 gtgccggagc tgctttcccg cctggttttc cgggtgcaga atccgccgtc cggcggcacg
      541 cagtacgatt ttgaacgctg ggcgcgtgaa gtgccggggg tgacgcgggc atggtgccgc
      601 ccggaatggc cgcaggcggg cagcgtgggg gtgacgttcg ttcaggacaa caacccggac
      661 atttttcccg gtgaaggtga tgtgaaacgt gtggcggatt atatccgcag tcatgatgat
      721 ccggcgaccg gtcagcctgt cggtcagccg ctggggccga cggtgaccgt gtttaaactg
      781 accaataagc cagtgccctt cagtatcagg atcatcccga aaacaccgga gaatcaggcg
      841 gccgtaaaac aggcactgac tgacctgtta tacaacgaat cccggcccgg cggtctggtt
      901 ctggcgtcgt ctttctggcg tgccgtggcg ggggtgaaaa atctggagga ttttgaagtg
      961 cgcagcccgc tgacgtcggt tcaggcgggg gacagtgagc tgctgacagt gg
//
LOCUS       DAZFVO020000404          971 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFVO020000404 DAZFVO020000000
VERSION     DAZFVO020000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.411"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..429
                     /locus_tag="ACHKNB_RS26505"
                     /old_locus_tag="pgaptmp_005301"
     CDS             <1..429
                     /locus_tag="ACHKNB_RS26505"
                     /old_locus_tag="pgaptmp_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGU4198610.1"
                     /translation="LFSLIMIIQLFVPYLLAVRKTEEWMISQMSFSGAILWINIFSSV
                     LLVPVYEEIVFRGCLFNSFKFWFNDNIYTSAIVTSVIFSALHQQYTDFRTFLMLFLVS
                     LVLISAKIKSNGLLMPILLHMSMNAVIAGIQYLASISYTH"
     gene            complement(604..882)
                     /gene="mcmA"
                     /locus_tag="ACHKNB_RS26510"
                     /old_locus_tag="pgaptmp_005302"
     CDS             complement(604..882)
                     /gene="mcmA"
                     /locus_tag="ACHKNB_RS26510"
                     /old_locus_tag="pgaptmp_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin McmA"
                     /protein_id="HGU4198611.1"
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIAG
                     AYIGDKVHSWATTATVSPSMSPSGIGLSSQFGSGRGTSSASSSAGSGS"
BASE COUNT          296 a          193 c          162 g          320 t
ORIGIN      
        1 cttttctctt taataatgat tattcagtta tttgtccctt atctgctggc agtgaggaaa
       61 acggaagaat ggatgatctc gcaaatgtct ttttccgggg ccattttatg gattaatata
      121 ttttcttccg tacttcttgt tcctgtatat gaagagattg tatttcgagg gtgtcttttt
      181 aactctttta aattctggtt taatgacaat atttacacct ctgctattgt aacatcagtc
      241 atattttctg cattacatca acaatatact gactttcgta catttttgat gctctttctg
      301 gtatcgttag ttcttattag tgcaaaaata aagtcgaatg gcctgttaat gcccatatta
      361 cttcacatgt cgatgaatgc agtcatagca ggaatacaat atctggcttc tatcagttat
      421 actcattaac agagtatttt ctgttacaat ctcatatgat aaaaattaaa cggactggca
      481 ttgttactat gcggtatgca taaaataata aacgttcgga ggagcctaac ttaaagcgtt
      541 acataggcac cattatcata taatgaagca cctgtaacat gtgcttcatt aacaatataa
      601 ggtttaactt ccactccccg cagacgaaga ggcacttgat gtacctctgc cggatccaaa
      661 ctgggatgat aatcctatac ctgagggaga catggaggga ctaaccgtcg cagtcgttgc
      721 ccatgaatgt actttatcac ctatataagc ccctgcaata gaaccaaatc ccgggctaat
      781 aaacgtacca gcaagtgaac caatagcaat taattctgcc tgcccgtcat tcccgtcacc
      841 tccagatatt tgttttattt cattttcaga tagttttctc atttcaacac cttcgctata
      901 agatttgtga agaagtaaaa attcacgttt aaccacaacg ataaaaatgt aactacaagt
      961 gataaaaaaa a
//
LOCUS       DAZFVO020000405          968 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFVO020000405 DAZFVO020000000
VERSION     DAZFVO020000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.412"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..116)
                     /locus_tag="ACHKNB_RS26515"
                     /old_locus_tag="pgaptmp_005303"
     CDS             complement(<1..116)
                     /locus_tag="ACHKNB_RS26515"
                     /old_locus_tag="pgaptmp_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4198612.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKT"
     gene            complement(130..316)
                     /locus_tag="ACHKNB_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /pseudo
     CDS             complement(130..316)
                     /locus_tag="ACHKNB_RS26520"
                     /old_locus_tag="pgaptmp_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            339..542
                     /locus_tag="ACHKNB_RS26525"
                     /old_locus_tag="pgaptmp_005305"
     CDS             339..542
                     /locus_tag="ACHKNB_RS26525"
                     /old_locus_tag="pgaptmp_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198613.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            622..>968
                     /locus_tag="ACHKNB_RS26530"
                     /old_locus_tag="pgaptmp_005306"
     CDS             622..>968
                     /locus_tag="ACHKNB_RS26530"
                     /old_locus_tag="pgaptmp_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198614.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEA"
BASE COUNT          274 a          191 c          224 g          279 t
ORIGIN      
        1 gttttaccac tcattttgtc ttcaaaaatc tgctcacatc ctgcacattc cagcgcattt
       61 ctttgtaaac cggtgttctg gtcatttgtt gatacgcgta tatagccaat cagcatgtat
      121 ttaccttcct taaaaagagg tgaaaatgcc ttttaaaaca gatatacggt atccctgaaa
      181 acctcggttt acgggaaacg gtaaataagg ctgctgatgc cctgccatcg ggtggaaccg
      241 ccgtggcggc aaacagactc gccaccccag taaatattaa tggtgttcct ttcgacggaa
      301 cgcgggatat aaatattaca tcggggatga cgcagtctgt ggctgactca cgttatgttc
      361 aaagcattcg aagaggttca aggtcaacga ttggaatgca atataatatt tttgaagttc
      421 ctgacggatg cgtacttaca gggcttgatg tcgctggtga tggtaatgca acggtaaccg
      481 catattatcg acctgttcag tttctgatag atggttcgtg gaaaacagct tccagcgcat
      541 aaaagtgcag tagttcaaaa aacagattat ctgaataaat atatgatgat tttgtctttt
      601 caacaaaaac aaggtgaaat aatggagctt attaacgtaa aacgatatta cccggaacac
      661 aaaccctatg gtgaggatgt tcagtatttc cagagtgagg acggaaggga tttttatgaa
      721 tctatcccgc ttttcacaaa gaaatacaaa ctctgtattt ctcctgtaac gggcattatc
      781 tgctctgttg ctgaagatgt ctctgcttta tatccggcgg gttttactgt tgttgaagtg
      841 gatgaactgc cggaaggtgt gaatattgac ggtaactggc agttttctga tggcctgata
      901 tcaaaggtgc cggtaaactg gaagacggtt gctgaaaagc gtcgctcatc gctgctccag
      961 gaagccaa
//
LOCUS       DAZFVO020000406          963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFVO020000406 DAZFVO020000000
VERSION     DAZFVO020000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.413"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>963
                     /locus_tag="ACHKNB_RS26535"
                     /old_locus_tag="pgaptmp_005307"
     CDS             <1..>963
                     /locus_tag="ACHKNB_RS26535"
                     /old_locus_tag="pgaptmp_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HGU4198615.1"
                     /translation="YGGATGLVRTLIWPFIFKNYSVRDLAEFLEVYGLPMKVGKYPSG
                     ATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQAAANGQADPFETMISWGERSISKAI
                     LGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLRQLAATLNRDLVYPLYALNTAHAID
                     IRRLPRICFQTKEPGDITKITSAVMQLSTGMDISDPWIREQTGIPQPTPGEAIFRVRQ
                     SGNEPAQTDKEMPPEKQGKTEQTALAARLPEAKSSPRDELDDMGDAVPARRLQEAIDP
                     VLEPVIDAIRTRGLAEALADLPALYREMDDSRLMTLLSDAMFAAE"
BASE COUNT          212 a          267 c          308 g          176 t
ORIGIN      
        1 tacggcggcg caacagggct tgtcagaacg cttatctggc cgttcatttt caaaaactat
       61 tccgtgcgcg atctggctga atttctggag gtgtacggcc tgccgatgaa ggtcggtaaa
      121 tacccgtccg gggcaacacc ggagcagaaa agcgccctga tgcgggcggt gatggatatc
      181 gggcgacgta caggcgggat catcccggcc gggatgtcgc tggagtttca ggcggccgcg
      241 aacggtcagg ccgatccgtt tgaaaccatg atttcgtggg gggagcgttc catctccaaa
      301 gctatcctcg gcggcacgct gaccacggaa gccggagaca agggcgcgcg ctcgctgggt
      361 gaagtgcata acgaggtgcg ccgggaaatc agggattctg atttacgtca gctggccgcc
      421 acgctgaacc gcgatctggt gtatccgctg tatgccctga acaccgccca cgccatagat
      481 atccgtcgcc tgccgcgtat ctgtttccag acaaaagagc cgggggatat caccaaaatc
      541 accagtgcgg tgatgcagct cagtacgggg atggatattt ctgatccctg gatacgtgag
      601 cagacgggta ttccgcagcc cacgcccggc gaagccatct tccgtgtccg tcagagtggc
      661 aatgaacctg ctcagacaga caaagagatg ccaccggaaa aacaggggaa gacagagcag
      721 acagcgctgg ctgccagact gccggaagcg aagagcagcc cccgagatga actggacgac
      781 atgggggatg cggtgcctgc ccgccggtta caggaggcca tcgacccggt actggagccg
      841 gtcattgatg ccatcagaac gcgggggctg gcggaggcac tggcagacct gcctgccctt
      901 taccgtgaaa tggatgattc ccgcctgatg acgctgctca gtgatgccat gtttgctgcg
      961 gaa
//
LOCUS       DAZFVO020000407          963 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFVO020000407 DAZFVO020000000
VERSION     DAZFVO020000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.414"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..369
                     /locus_tag="ACHKNB_RS26540"
                     /old_locus_tag="pgaptmp_005308"
     CDS             <1..369
                     /locus_tag="ACHKNB_RS26540"
                     /old_locus_tag="pgaptmp_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4198616.1"
                     /translation="SGKQFSWKDHGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVT
                     ARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFEK
                     SAFGKYNVDNAVNASFRYSF"
     gene            complement(792..>963)
                     /locus_tag="ACHKNB_RS26545"
                     /old_locus_tag="pgaptmp_005309"
     CDS             complement(792..>963)
                     /locus_tag="ACHKNB_RS26545"
                     /old_locus_tag="pgaptmp_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HGU4198617.1"
                     /translation="YSHSINNGYDHVDVIVRTTSNDGLTILRVVNKESAHEFVQETLQ
                     EAWETAEDCFIQ"
BASE COUNT          259 a          195 c          251 g          258 t
ORIGIN      
        1 tccggtaaac agttcagctg gaaggaccac gggatgagcc tgtccatgaa ggacaaggac
       61 tacaatccgc tgattggccg taccggtgtg gatgtgggta aatcgttctc cggtaaggac
      121 tggaaagtga cggcacgtgc tggtcttggc tatcagtttg acctgctggc gaacggagaa
      181 acggtactgc gtgatgcatc cggagagaag cgtattaagg gcgggaaaga cggtcgcatg
      241 ctgatgtctg tgggtctgaa cgcagaggtt cgtgacaaca tccgttttgg tctggagttt
      301 gagaaatcag cctttggtaa gtacaacgtt gataatgctg tcaacgccag cttccgttat
      361 tcattctgac ggttgttact gaaggggatt atggcctgac ggctccctgt aaataaagac
      421 aggcacagaa gattgtgagt tacttagtgt tccaccaaaa agtaaggccg caactctgtg
      481 cctgcttcgt aagtgtacag tgagggccat atggtcataa ctgaagcatc cgacgatgtg
      541 gtgtctaccc aaaaagcaac ctgtgaatat taggctggtt aagtgcgtat tttcatggaa
      601 ggagatcacc caatagcccc gatctccttt atgttaatac gcattaagcg ggtaaaacag
      661 gaagattaag aaagggagag tttttcgact ccccaaccaa gagaagcagc ttataactac
      721 cagatagttg ttatgtcata cgtgacggat aatgtcataa aaaatatgtc attgccgtga
      781 ttataatcgc gttattggat aaagcagtct tcagcggttt cccaggcttc ctgcagcgtt
      841 tcctgtacaa attcgtgcgc ggattcttta tttacaactc ttaaaatggt gagtccatca
      901 ttgctggtgg ttctgacaat cacgtccaca tggtcatatc cgttatttat ggaatgacta
      961 taa
//
LOCUS       DAZFVO020000408          956 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFVO020000408 DAZFVO020000000
VERSION     DAZFVO020000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.415"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>956
                     /locus_tag="ACHKNB_RS26550"
                     /old_locus_tag="pgaptmp_005310"
     CDS             <1..>956
                     /locus_tag="ACHKNB_RS26550"
                     /old_locus_tag="pgaptmp_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGU4198618.1"
                     /translation="QGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARSFT
                     AAKVARLDVLTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNAEE
                     VQLGNPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSALNG
                     LVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGVDK
                     QTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREMVV
                     QEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQA"
BASE COUNT          223 a          263 c          292 g          178 t
ORIGIN      
        1 caggggattg atttaggtta tgccgccacg cttccctcaa aagaggcggt ggcatacttc
       61 cgcgccaaag gggcgcatat cagctggaac tggttcgaaa cagacgcgga tgttcatgcc
      121 cgttcgttca cggcggcaaa agtggcacgc ctggacgtac tgactacact acaggcggaa
      181 gtgcaacggg ctattgatga ggggatttca cagaaagcat ttatccgcac actgaccccc
      241 cgcctgcaaa agctgggatg gtggggaaag cagattgtgg tggacagcgc cggtaacgcg
      301 gaagaagtgc agctgggtaa tccccgccgt ctggcgctga tttacaacgt gaacacccgc
      361 gtggcttaca atgccgggcg ttacacgcag atgatgaaca acacggacac gcatccgttc
      421 tggcagtatg tggcggtgat ggacagccgc acccgcccgg cgcattccgc ccttaacggg
      481 ctggtattcc gctatgacga tccgttctgg aatacacact acccacccaa tggctggaac
      541 tgccgctgcc gtgtccggcc attgtctcag gcccgtctgg atgcaatggg gttatccgtt
      601 tcatccggtc aggatcatct ctccacccgc aatgttgagg ctggcgtgga taagcagacc
      661 ggggaagtca gagaaatgcc ggtgaccaca tattcagatg gcaccagaac catgacaccg
      721 gatgtgggct ggtcatataa ccccggctcg gcggcgttcg gcacagacca ggcgctgatc
      781 cgtaaactga tcgaggtgaa aagcccggcg ctgcgggaaa tggtggtgca ggagatgaac
      841 aacagcccgg agcggcagct ggcgttccgc atctgggcaa aaaacatcat gaagacccgg
      901 cgaggcggta acgatatccg cacgctgggc tttatgaccg aaagcattgc gcaggc
//
LOCUS       DAZFVO020000409          953 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFVO020000409 DAZFVO020000000
VERSION     DAZFVO020000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.416"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>953
                     /locus_tag="ACHKNB_RS26555"
                     /old_locus_tag="pgaptmp_005311"
     CDS             <1..>953
                     /locus_tag="ACHKNB_RS26555"
                     /old_locus_tag="pgaptmp_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4198619.1"
                     /translation="EGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGF
                     VMASPENFRFSEERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLA
                     ITGDPGLTGMNPVAALSADDLYNALTPPLKDTSMNEQLRQLLTALGLTVADGDEFTPE
                     LGTAALSALTGIKTRADAHDTLKTQVASLSAELETAKGTPAGSSIDLTKYVPVETYNA
                     LRTEYVALSAQHGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQ
                     PVAALTGLQTDTVTVPDKKTATAVLSAEDIAAAHLLGKTEA"
BASE COUNT          210 a          279 c          277 g          187 t
ORIGIN      
        1 gaaggctggc tgattaaccg tgcgaccgtt gagcgcatgg tatcccgggt tgtggcgctc
       61 aatcagccgg taaaaattga ctacaaccac cagaccctga ttaaggggca tcaggcaccg
      121 gctgccggtt ttgtgatggc ttcaccggaa aacttccgct tcagtgaaga gcgtggcttc
      181 gaggtgcgcc cgaagtggaa ccctccggcc cttgaacatc tgcgtaataa cgaattcccc
      241 tggttttcac cggtgattgg ctatgacgag agcaccggcg aacctgtcga acttcggatg
      301 ctggctatta ccggtgaccc cggtctgacc ggcatgaatc ctgtcgccgc gctgtcggcg
      361 gatgaccttt ataacgcctt aacccctcct ttaaaggata cctccatgaa tgagcaatta
      421 cgccagttgc tgacggcgct cggtctgacc gtggctgacg gtgacgaatt tacgccggaa
      481 ctgggcacgg cggcgctgtc tgcccttacc gggatcaaaa cccgtgcgga tgcgcacgac
      541 accctgaaaa cgcaggttgc cagcctgtcg gcagagctgg aaaccgcaaa aggcacaccg
      601 gctggcagca gtattgatct gacgaaatac gtgcccgttg aaacgtataa cgccctgcgt
      661 accgaatacg ttgcactgtc ggcgcagcac ggcagcacca cgctggagca ggtgctggac
      721 aaggctgaat ccgaagggcg catcttcaaa agcgaacgcg gctatctgga acaactgggt
      781 gggcagattg gtgttgctgc actttctgca cagcttgacg cccgacagcc ggttgcggcc
      841 ctgaccggcc ttcagaccga caccgtgacc gtgccggata aaaagactgc caccgctgtg
      901 ctgtcggctg aagatatcgc cgccgcccat ctgctgggta aaaccgaagc cga
//
LOCUS       DAZFVO020000410          950 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFVO020000410 DAZFVO020000000
VERSION     DAZFVO020000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.417"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(739..>950)
                     /locus_tag="ACHKNB_RS26560"
                     /old_locus_tag="pgaptmp_005312"
     CDS             complement(739..>950)
                     /locus_tag="ACHKNB_RS26560"
                     /old_locus_tag="pgaptmp_005312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198620.1"
                     /translation="LSIYHVICSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNG
                     YFMERSNFLVAIIMLILRSLAIIPG"
BASE COUNT          293 a          170 c          191 g          296 t
ORIGIN      
        1 tgaatgttcg taagctatat cacttactca attcatttta cccagagtca taaaatagag
       61 tgtctgttga tctcattttc atattccttt cagactgaag tgcattttca tcacccgatg
      121 agaaagaact gaagtgatgt ttgccataga ttacgagatg cccagtctca ttgcctatcg
      181 tggtgcacct ttatacctgt tataccagat caaaaatcac gcaatccata caacgaaacc
      241 agatttgcaa ttcgtgtcac aaaatatgtc gatctttttc taagaagaag atgccatgtg
      301 aagccagacg aacacttgcg gaggccttca caaaccaaag attgttgttt aattatctgt
      361 tttataagta atttatttaa agtataaaca aggaaagatt tgcaagctaa ctcagtgagc
      421 gtggtgaaaa tcagtgttta cccgccatca ggctgagcat aattctcatc atgaaatatg
      481 tttcctggtt tgtggcttgt aactggtcac ttctgaagtc gatctggaga ggcttgttga
      541 tgttggtgtt ttcaggatga tgtttcactt agtttgtttg ccgtatcgcc cggcgaatgg
      601 ctgtgattga ggaaggttta agtcgtagtg accaaagcta tatttaccaa cgaatgtaga
      661 tgaaaaaatc atctcctgcg ttccccatat ctccaggata aaaaggaatg taacaatctc
      721 atggcgtaag ctgacgaatc agccaggaat aatcgctagg gacctaagaa ttagcatgat
      781 aatagccact aagaaattac tgcgctccat gaaatagcca ttttgtggca aatggagttg
      841 actaataatg tcatatgtga gacggctagt tgaacgaata ttaaattttg ctgaattttt
      901 tatgttgatt ttacttgtta cagaacatat cacatgatat atagataaga
//
LOCUS       DAZFVO020000411          941 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFVO020000411 DAZFVO020000000
VERSION     DAZFVO020000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.418"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..818
                     /gene="gadX"
                     /locus_tag="ACHKNB_RS26565"
                     /old_locus_tag="pgaptmp_005313"
     CDS             <1..818
                     /gene="gadX"
                     /locus_tag="ACHKNB_RS26565"
                     /old_locus_tag="pgaptmp_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HGU4198621.1"
                     /translation="LHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKC
                     VENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKN
                     LRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLAR
                     IASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSV
                     SYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          271 a          181 c          213 g          276 t
ORIGIN      
        1 cattacatgg gaattgtcta attgcgtacg caagacataa atatattctc accatggtta
       61 atggtgaata tcgctatttt aatggcggtg acctggtttt tgcggatgca agccaaattc
      121 gagtagataa gtgtgttgaa aattttgtat tagtatcaag ggatacgctt tcattatttc
      181 tgccgatgct caaggaggag gcattaaatc ttcatgcaca taaaaaaatt tcttcattac
      241 tcgttcatca ctgtagcaga gatattcctg tttttcagga agttgcgcaa ctatcgcaga
      301 ataagaatct tcgctatgca gaaatgctac gtaaaagagc attaatcttt gcgttgttat
      361 ctgtttttct tgaggatgag cactttatac cgctgctttt gaacgttttg caaccgaaca
      421 tgcgaacacg agtttgtacg gttatcaata ataatatcgc ccatgagtgg acactagccc
      481 gaatcgccag cgagctgttg atgagtccaa gcctgttaaa gaaaaaattg cgcgaagaag
      541 agacatcata ttcacagttg cttactgagt gtagaatgca acgtgctttg caacttattg
      601 ttatacatgg cttttcaatt aagcgagtcg cagtgtcctg tggatatcac agcgtgtcgt
      661 atttcattta cgtctttcga aattattatg ggatgacgcc cacagagtat caggagcgat
      721 cggcgcaggg attgccgaac cgtgactcgg cggcaagtat tgttgcgcaa ggaaattttt
      781 acggcactga ccgttctgcg gaaggaataa gattatagaa ttttactcag acataaaaaa
      841 aacccggcat aggggaccgg gaagaggata gtctgccgtc tccagactaa taaaccgtta
      901 taacactccc tgttggcacg ggaaactttg tgctctcagt a
//
LOCUS       DAZFVO020000412          939 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFVO020000412 DAZFVO020000000
VERSION     DAZFVO020000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.419"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>939
                     /locus_tag="ACHKNB_RS26570"
                     /old_locus_tag="pgaptmp_005314"
     CDS             <1..>939
                     /locus_tag="ACHKNB_RS26570"
                     /old_locus_tag="pgaptmp_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198622.1"
                     /translation="QARESAHQYALTIDEIAHKTPSMSLPEASDNEGRTREALTEQNR
                     LIDEQASRVKSLQEKIAGYQYVLANPGWTTGNGFMINHLTSVKTVTEGLAQATEQFAV
                     EQSRLAQMQEKAQSIQDVLAGLEERRVVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLL
                     GLGNELLQQRQGLASVPLRLPQATLDDKQQGALNNTERQLALSRLKGEEKERARLGYA
                     ADDLGLVGDTYQEARQRYIRNSMEAWRNNEANKPKSRAGKSGAEKAEDSFSRLLKQQK
                     EQLALAGKNTELAKLKYQTSQGELKTLTEIQKQELLR"
BASE COUNT          260 a          225 c          293 g          161 t
ORIGIN      
        1 caggccaggg agtctgcgca ccagtatgca ctgacgattg atgaaatcgc gcataaaacg
       61 ccatcaatgt ctctgcctga agcctcagat aatgaagggc gaacacggga ggcgctgaca
      121 gagcagaacc ggctgattga tgagcaggcc agccgggtga aatccctgca ggaaaaaatc
      181 gccgggtatc agtatgtgct ggctaatccg ggctggacga ccggtaacgg cttcatgata
      241 aaccatctga catcggtgaa aactgtaacg gaagggcttg ctcaggcaac agagcagttt
      301 gctgttgagc agtcccgtct ggcacagatg caggaaaaag cgcagtccat tcaggatgtg
      361 cttgccggac tggaagagcg ccgtgttgtg ttaattcgtc agcaggcggc agagcagaat
      421 aaagcgtatc agtcactgct gatcatgaac ggtcagcata cggaattcaa ccgcctgctg
      481 gggctgggta atgaactgct gcaacagcgt cagggactgg cgagtgtacc gctgcgactg
      541 ccacaggcca ctctggatga taaacagcag ggcgccctga ataacacaga gcgtcaactt
      601 gccctgtccc ggctgaaagg ggaagaaaaa gagcgtgccc ggctggggta tgcggcggat
      661 gaccttggtc tggtggggga tacgtatcag gaggcgaggc agcgttacat ccgtaattcg
      721 atggaagcct ggcgcaataa tgaggcgaat aaacccaaat cccgggccgg aaaatcaggg
      781 gcggaaaaag cggaagacag tttttcccgt ctgctgaagc agcagaagga acaactggca
      841 ctggccggga agaatacaga actggcaaaa ctgaaatacc agacctcgca gggcgagctg
      901 aaaaccctga cggagataca gaagcaggag ctgctgcgc
//
LOCUS       DAZFVO020000413          936 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFVO020000413 DAZFVO020000000
VERSION     DAZFVO020000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.420"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            105..701
                     /gene="fimE"
                     /locus_tag="ACHKNB_RS26575"
                     /old_locus_tag="pgaptmp_005315"
     CDS             105..701
                     /gene="fimE"
                     /locus_tag="ACHKNB_RS26575"
                     /old_locus_tag="pgaptmp_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HGU4198623.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          265 a          184 c          203 g          284 t
ORIGIN      
        1 cacttcgtgt gtggttatct ttttatctat tgggctaatt ttgaccgatt gaggtttcct
       61 ataggtattc attcaaatat atctcagtta ggagtactac tattgtgagt aaacgtcgtt
      121 atcttaccgg taaagaagtt caggccatga tgcaggcggt ttgttacggg gcaacgggag
      181 ccagagatta ttgtcttatt ctgttggcat atcggcatgg gatgcgtatt agtgaactgc
      241 ttgatctgca ttatcaggat cttgacctta atgaaggtcg aataaatatt cgccgactga
      301 agaacggatt ttctaccgtt cacccgttac gttttgatga gcgtgaagcc gtggaacgct
      361 ggacccagga acgtgctaac tggaaaggcg ctgaccggac cgacgctata tttatttctc
      421 gccgcgggag tcggctttct cgccagcagg cctatcgcat tattcgcgat gccggtattg
      481 aagctggaac cgtaacgcag actcatcctc atatgttaag gcatgcttgt ggttatgaac
      541 tggcggagcg tggtgccgat acccgtttaa ttcaggatta tctcgggcat cgaaatattc
      601 gccatactgt acgttatacc gccagtaatg ctgctcgttt tgccggacta tgggaaagaa
      661 ataatctcat aaacgaaaaa ttaaaaagag aagaggtttg atttaactca ttgataataa
      721 agttaaaaaa caaataaata caagacaatt ggggccaaac tgtccatatc ataaataagt
      781 tacgtatttt ttctcaagca taaaaatatt aaaaaacgac aaaaagcatc taactgtttg
      841 atatgtaaat tatttctctt gtaaattaat ttcacatcac ctccgctata tgtaaagcta
      901 acgtttctgt ggctcgacgc atcttcctca ttcttc
//
LOCUS       DAZFVO020000414          934 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFVO020000414 DAZFVO020000000
VERSION     DAZFVO020000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.421"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..398
                     /locus_tag="ACHKNB_RS26580"
                     /old_locus_tag="pgaptmp_005316"
     CDS             <1..398
                     /locus_tag="ACHKNB_RS26580"
                     /old_locus_tag="pgaptmp_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030598 - rRNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome-inactivating family protein"
                     /protein_id="HGU4198624.1"
                     /translation="EALRFRQIQREFRLALSETAPVYTMTPEEVDLTLNWGRISNVLP
                     EFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNEEIQPECQITGDRPV
                     IRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            411..674
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS26585"
                     /old_locus_tag="pgaptmp_005317"
     CDS             411..674
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS26585"
                     /old_locus_tag="pgaptmp_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4198625.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          270 a          173 c          242 g          249 t
ORIGIN      
        1 cagaagcctt acggttcagg caaatacaga gagaatttcg tctggcactg tctgaaactg
       61 ctcctgttta tacgatgaca ccggaagaag tggacctcac actgaactgg gggagaatca
      121 gcaatgtgct tccggagttt cggggagagg gtggtgtcag agtggggcga atatccttta
      181 ataatatatc agcgatactg ggcacagtgg cggttatact gaattgccat catcaggggg
      241 cacgttccgt tcgctccgtg aatgaagaga tacaaccaga atgtcagata actggcgaca
      301 ggccagttat aaggctaaac aatactttat gggaaagtaa taccgcagct gcttttctga
      361 atcgcagggc tcactcttta aatacatccg gagaataacg ggagttaaat atgaagaaga
      421 tatttgtagc ggctttattt gcttttgttt ctgttaatgc aatggcagct gattgtgcaa
      481 aaggtaaaat tgagttctct aagtataatg agaatgatac attcacagta aaagtggccg
      541 ggaaagagta ctggactaac cgctggaatc tgcaaccgct actgcaaagc gcacagttaa
      601 caggaatgac ggtaacaatc aaatcaaata cctgtgcgtc aggttcagga tttgctgaag
      661 tgcagtttaa ttaatatcag aagcattgct ggtttcgtag tgtgcagcaa tgtagttaca
      721 gtgtaatcaa tgtcacaatt cagtcagttg acggttgcct gtctggctga gcatttgtta
      781 aaaaaatcct gcatgatgaa tccccctgtg cggaggggca taatgacaga tgtttggttg
      841 cgtattgtat aggcaagttg cggattctgt ctggtcattg cagaattcac cgggaggcac
      901 ccggcatcat gctgtataca gagattaggc atat
//
LOCUS       DAZFVO020000415          927 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFVO020000415 DAZFVO020000000
VERSION     DAZFVO020000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.422"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..781)
                     /gene="allD"
                     /locus_tag="ACHKNB_RS26590"
                     /old_locus_tag="pgaptmp_005318"
     CDS             complement(<1..781)
                     /gene="allD"
                     /locus_tag="ACHKNB_RS26590"
                     /old_locus_tag="pgaptmp_005318"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HGU4198626.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRN"
BASE COUNT          214 a          252 c          236 g          225 t
ORIGIN      
        1 aattacgccc tgcgtgtaaa tcgtcataca tcgaactaac ctgtcgcccg aacggtaagc
       61 cgagtaagac gcctgagagg acgtcaatca tcatcatcag gccataccct tttggcccgg
      121 cggcggggag cagagcatgt accgcgaacg gatcggttgt tggtgcaccg tttttatcga
      181 ccgcccaggt atccgggata gacatattac gcgagcgggc gtcgagcact tttccccatg
      241 cctgtacggt agtcgccata tcaaaggtaa ggatctcgtc gccttctccc ggcgcggcaa
      301 aggccagggg gttagtaccg tagtaaattt ccgcgccgcc aaacggcacc accattggat
      361 cggactggca catcgaaatg ccaatgaatc cggcgcgggc tgcctgctgc acaaaataag
      421 agattgcgcc gctgtgaccc atccggctga taccgaccac cgcaacgcca ttttgctggg
      481 cggttttgat ggcatgttcc atacccattt tcgccgcgac ctgtccggcg gcattgtcgg
      541 catgtaaaat tgccgagcac ggcccggttt cctcaagacg aaactccggt tcgcggttgg
      601 tgccgccttt tgaaatgcgt tccgcgtagt attccacgcg caccgcgcca tgagagtgga
      661 tccctctggc atcggcgtaa accaatactt cagccacggt tgcagcgtgc tcacgtttta
      721 acccagcctg gcagagttta ttctcaatta gctggtggag tgtttcccga ctgattttca
      781 tctgtcttcc tttttaacga cggtgtgaag catgactgca attaacatac agggaaaata
      841 tctggattat gtgatccaga caggcaaaaa aatatagtta gaatttattt gataatccgc
      901 tcgcttttaa cctggttttt aaaacaa
//
LOCUS       DAZFVO020000416          926 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFVO020000416 DAZFVO020000000
VERSION     DAZFVO020000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.423"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>926
                     /locus_tag="ACHKNB_RS26595"
                     /old_locus_tag="pgaptmp_005319"
     CDS             <1..>926
                     /locus_tag="ACHKNB_RS26595"
                     /old_locus_tag="pgaptmp_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HGU4198627.1"
                     /translation="LPSKEAVAYFRAKGAHISWNWFETAADVHARSFTAAKAARLDVL
                     TTLQAEVQRAIDEGISQKAFISTLTPRLQKLGWWGKQIVVDSAGNAEEVQLGSPRRLA
                     LIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDNRTRPAHSALNGLVFRYDDPFW
                     NTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGVDKQTGEVREMPV
                     TTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREMVVQEMNNSPERQ
                     LAFRIWAKNIMKTRRGGNDIRTLGFITESIAQA"
BASE COUNT          219 a          256 c          279 g          172 t
ORIGIN      
        1 cttccctcaa aagaggcggt ggcatacttc cgcgccaaag gggcgcatat cagctggaac
       61 tggttcgaaa cagccgcgga tgttcatgcc cgttcgttca cggcggcaaa agcggcacgc
      121 ctggacgtac tgaccacact acaggcggaa gtgcaacggg ccattgatga ggggatttca
      181 cagaaagcat ttatcagcac gctgacaccc cgcctgcaaa agctgggatg gtggggaaaa
      241 cagattgtgg tggacagcgc cggtaacgca gaagaagtgc agctgggcag tccccgccgt
      301 ctggcgctga tttacaacgt gaacacccgc gtggcttaca atgccgggcg ttacacgcag
      361 atgatgaaca acacggacac gcatccgttc tggcagtatg tggcggtgat ggacaaccgc
      421 acccgcccgg cgcattccgc ccttaacggg ctggtattcc gctatgacga tccgttctgg
      481 aatacacact acccgcccaa tggctggaac tgtcgctgtc gtgtccggcc attgtctcag
      541 gcccgtctgg atgcgatggg gctgtctgtt tcatccggtc aggatcatct ctccacccgc
      601 aatgttgagg ctggcgtgga taagcagacc ggagaagtca gagaaatgcc ggtgaccaca
      661 tattcagatg gcaccagaac catgacaccg gatgtgggct ggtcatataa ccccggttcg
      721 gcggcgttcg gcacagacca ggcgctgatc cgtaaactga tcgaggtaaa aagcccggcg
      781 ttacgggaaa tggtggttca ggagatgaac aacagcccgg agcggcagct ggcgttccgt
      841 atctgggcca aaaacatcat gaagacccgg cgaggcggta acgatatccg cacgctgggc
      901 tttataaccg aaagcattgc gcaggc
//
LOCUS       DAZFVO020000417          920 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFVO020000417 DAZFVO020000000
VERSION     DAZFVO020000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.424"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..873
                     /locus_tag="ACHKNB_RS26600"
                     /old_locus_tag="pgaptmp_005320"
     CDS             <1..873
                     /locus_tag="ACHKNB_RS26600"
                     /old_locus_tag="pgaptmp_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGU4198628.1"
                     /translation="TQYVIAREAGISDGTLSAFRKGKYKGDNDAIAATMLSWYKDWKK
                     RPSLPEPPQFVETPTVRELRAVFQAARLMGCINIIVGVPGVGKTVTASNYCQEQPNTW
                     MITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAIRRRLMGTRGLVIVDEADHLG
                     IDGLEQIRAIQDATSVGMVLIGNPKGLFSESRSDTNNLARLFSRIARVKQLHEAKKED
                     VLDIAGAWGISGEEELDAMQAIAEKPGALRVLTHTLNQAWIAASGEGTTLTKKHINTA
                     FKEVYTEPDSLLKV"
BASE COUNT          234 a          212 c          272 g          202 t
ORIGIN      
        1 acgcagtatg tcatcgccag agaggcggga atttccgacg gcacattatc ggctttccgc
       61 aaagggaaat ataaaggaga caatgatgct atagcagcga ctatgctctc ctggtacaaa
      121 gactggaaaa agcggccttc cctgccggaa ccgccgcagt ttgtggagac accgacggta
      181 cgggaactgc gggcggtgtt tcaggcggca cgcctgatgg gctgtattaa catcattgtg
      241 ggcgtaccag gtgtgggtaa aacagtcacc gccagcaatt attgtcagga gcaaccgaac
      301 acctggatga tcaccctgtc tcccgcgcat tccagcgtca ctgagtgtct gctggagctg
      361 gcagaggagc tggggattga ttacacccgc gcgaacaagg gggcgttatc ccgcgccatc
      421 cgccgtcgcc tgatgggaac gcgtggactg gtgattgtgg atgaggcgga tcatcttggt
      481 attgacggtc tggagcagat ccgggcaatc caggacgcca cgagtgtagg gatggtgctt
      541 atcggtaacc cgaaaggact gttctcagaa tcgcgtagtg acacgaataa tttagcacgc
      601 ctgttcagcc gtattgccag ggttaaacag ttgcatgagg ccaaaaaaga ggatgtgctg
      661 gatattgccg gagcatgggg tatcagtgga gaggaagagc tggacgccat gcaggctatc
      721 gctgaaaaac cgggagcatt acgcgttctg acacatacgc ttaaccaggc atggatcgct
      781 gccagcggtg aaggtacgac gctgacaaaa aaacacatta atacggcctt taaagaggtt
      841 tatacagaac ccgattcgct attaaaggtg tgattatggt tgtatttaat attcctgata
      901 tttacggacg cttttacctg
//
LOCUS       DAZFVO020000418          918 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFVO020000418 DAZFVO020000000
VERSION     DAZFVO020000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.425"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>918
                     /locus_tag="ACHKNB_RS26605"
                     /old_locus_tag="pgaptmp_005321"
     CDS             <1..>918
                     /locus_tag="ACHKNB_RS26605"
                     /old_locus_tag="pgaptmp_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198629.1"
                     /translation="QLAALGDKVTYQERLNALAQKADKFAQQQRAKRAAIDAKSRGLT
                     DRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDLLRGNWMAGLRSGWSEWKE
                     SATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGI
                     VGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVF
                     TKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGTNG
                     LPGPQMMKAVYDMARKGARDEIQAQMRDGG"
BASE COUNT          216 a          216 c          319 g          167 t
ORIGIN      
        1 ccagctggct gcacttggcg acaaggttac gtatcaggag cgcctgaacg cgctggcgca
       61 gaaggcggat aaattcgcac agcagcaacg ggcaaaacgg gccgctattg atgcgaaaag
      121 ccgggggctg actgaccggc aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca
      181 gtatggcgat aatcctctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc
      241 ggctgaagac ctgcttcgcg ggaactggat ggcaggcctc aggtccggct ggagcgagtg
      301 gaaagagagt gccacggaca gtatgtcgca ggttaaaagt gcagccacgc agacctttga
      361 tggtattgcg cagaatatgg cggcgatgct gaccggcagt gaacagaact ggcgcagctt
      421 cacccgctcc gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat
      481 tgtcgggagt atcggcagcg ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg
      541 cggtacagcc attcaggctg ctgcggcgaa attccatttt gcgaccgggg gatttacggg
      601 aaccggcggc aaatatgagc cagcggggat tgttcaccgt ggtgaatttg tcttcacgaa
      661 ggaggcaacc agccggattg gtgtcggcaa cctgtaccgc ctgatgcggg gctatgcgga
      721 aggtggttat gtgggcggtg ccggaagtcc ggcgcagatg cggcgggctg aaggcattaa
      781 ttttaatcag aacaatcacg tggtgattca gaacgacggt acgaatggtc tgccaggtcc
      841 acagatgatg aaggcggtgt atgacatggc ccgcaagggt gcccgtgatg aaatccaggc
      901 acagatgcgc gatggtgg
//
LOCUS       DAZFVO020000419          900 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFVO020000419 DAZFVO020000000
VERSION     DAZFVO020000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.426"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            228..830
                     /gene="fimB"
                     /locus_tag="ACHKNB_RS26610"
                     /old_locus_tag="pgaptmp_005322"
     CDS             228..830
                     /gene="fimB"
                     /locus_tag="ACHKNB_RS26610"
                     /old_locus_tag="pgaptmp_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HGU4198630.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          270 a          166 c          195 g          269 t
ORIGIN      
        1 attgataaat atataaaggt acatagcatg caagagcatg gcgtttgtat ggcaacgtta
       61 ttataattaa ctgttgctac tccatttaag ttcactcaga agaactggtc cacttacgtt
      121 agttattaag caaacgttcg cttttataaa cataatcagg ataaaaatgt tggattattg
      181 ctaacccagc acagctagtg cgcgtctgta attataaggg aaaaacgatg aagaataagg
      241 ctgataacaa aaaaaggaac ttcctgaccc atagtgaaat cgaatcactc cttaaagcag
      301 caaataccgg gcctcatgca gcacgtaatt attgtctgac tttgctttgt tttattcatg
      361 gtttccgggc gagtgaaatt tgtcgattga ggatttcgga tattgatctt aaggcaaagt
      421 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcatccgcta ttgaacaaag
      481 aagttcaggc tttaaaaaac tggttgagta tccgtacttc gtacccgcat gctgagagcg
      541 agtgggtatt tttatctcgt aagggaaatc cgctttctcg gcaacagttt taccatatta
      601 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgcc
      661 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
      721 ttgggcatcg caatattcgt catactgtct ggtataccgc cagcaatgca gggcgttttt
      781 acggcatctg ggatagagcc agaggacgac agcgtcacgc tgttttatag agatagttga
      841 gatactaggg atggtgttaa aaactacatt attcttacga tatatcaaaa atgattaacc
//
LOCUS       DAZFVO020000420          883 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFVO020000420 DAZFVO020000000
VERSION     DAZFVO020000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.427"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            123..692
                     /locus_tag="ACHKNB_RS26615"
                     /old_locus_tag="pgaptmp_005323"
     CDS             123..692
                     /locus_tag="ACHKNB_RS26615"
                     /old_locus_tag="pgaptmp_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4198631.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVKPSRVIEETISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAVNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
BASE COUNT          276 a          166 c          201 g          240 t
ORIGIN      
        1 aacacccgtt aagtgtctat ataaaaaggt agctgtagtt atagacaaca attgtgataa
       61 ttgattaaat gtctatagac aaacaaaaca gacctttgtt ggctatatag agagataaga
      121 acatgcgtac attcctttat gctcgtgtat cagttgatga cctcaccaca cagaaccaag
      181 tagaagctgt taaacgtgct ggttatgaag tcaaaccttc acgagtgatt gaggaaacca
      241 ttagtggtgc taccccagct atggacagac cacaatttgt aaagctggtg gataagctgg
      301 aagaaggtga ttgtcttgta gtgaccaaga ttgaccgctt aggaagggat aacattgacg
      361 tacagaagac cgtcacaatg ttacttggta tgggtgttaa gctggtctgt ctagatttgc
      421 ctgtccaaga cctttcaagt gctgaaggta aacttattct tcaactattc agtacctttg
      481 cagagtttga acttaaccgc atcagagagc gaaccagaga gggtttagaa cgagctaaag
      541 cacaaggtaa ggtgttaggt cgccctgtag ctgtgaacac tacagaggcc gttttagagc
      601 ataaggcaaa gggattatca caaagtcagg ttgcaaagct tctgaatctc tctgtaagaa
      661 cggttagccg tcactggaca aagacactgt aaaaaaaact tagtagcatc ttcatagcct
      721 ccttcgtggg ggcttttttg tgtctgtaac atatggtgac caactgcata gatagcgtta
      781 tatgctcctg tatatagcac ttttctagga gtcgagagag ggacgactaa cacgatacag
      841 tttaccctga aaagagactt cacagattct ctatatattt caa
//
LOCUS       DAZFVO020000421          871 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFVO020000421 DAZFVO020000000
VERSION     DAZFVO020000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.428"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..711
                     /locus_tag="ACHKNB_RS26620"
                     /old_locus_tag="pgaptmp_005324"
     CDS             <1..711
                     /locus_tag="ACHKNB_RS26620"
                     /old_locus_tag="pgaptmp_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HGU4198632.1"
                     /translation="LLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNLPTFSLTPEGRLTARNADISGHISANSGTLNNVTINQNCRILGKLSANQIEGDI
                     VKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYS
                     SCRLMVKCNGRVLFERTALAESQVYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASI
                     SDLLVVVMKKSTAGISIS"
BASE COUNT          200 a          210 c          269 g          192 t
ORIGIN      
        1 ctgctgcagg cggaccgtat cgcgatgatt aacccggaga acggcaacac cacgccgctg
       61 tttgtggcgc aggggaatca gctgttcatg aacgatgtgt tcctgaagcg gttgtttgcg
      121 gtgagcatca cctcgtccgg caatctcccg acgttctccc tgacgccgga gggcaggctg
      181 acggcccgca atgcggacat cagcggacat atcagtgcga actcgggcac gctcaataat
      241 gtgaccatta accagaactg ccggatactt ggaaaactgt ctgccaacca gattgaaggc
      301 gatattgtta aaaccgtggg gaaggctttc ccgcgggact cccgcgcacc ggagcggtgg
      361 ccatcgggga ccattaccgt caggatttat gacgatcagc cttttgaccg gcaaattgtg
      421 atcccggcag tggctttcag cggcgctaaa catgagagag agcataacga tatttattca
      481 tcatgccggc tgatggtgaa atgtaacggc agggtgcttt ttgaaaggac ggcgctggct
      541 gagtctcagg tgtattcagg ggtgattgat atgcctgcag gaagggggca tatgacgctg
      601 gagttttctg tgtctgcctg gctggtgaat ggatggtacc cgacagcgag tatcagcgat
      661 ctgctggttg tcgtgatgaa gaagtccacg gcgggtatca gtatttcgtg atcatgagga
      721 gccggattgc cggaaggaga caaaaaccgt acattatgcg cgggtgcctt tggctgatgg
      781 ccggagggag cacctgaagg ccggatgtga aaaggccccg gacaaacatt catgtttaac
      841 ccgaggcctg acgttcctac cttcaacaag t
//
LOCUS       DAZFVO020000422          865 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFVO020000422 DAZFVO020000000
VERSION     DAZFVO020000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.429"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..464
                     /locus_tag="ACHKNB_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /pseudo
     CDS             1..464
                     /locus_tag="ACHKNB_RS26625"
                     /old_locus_tag="pgaptmp_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(525..818)
                     /locus_tag="ACHKNB_RS26630"
                     /old_locus_tag="pgaptmp_005326"
     CDS             complement(525..818)
                     /locus_tag="ACHKNB_RS26630"
                     /old_locus_tag="pgaptmp_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HGU4198633.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
BASE COUNT          285 a          148 c          166 g          266 t
ORIGIN      
        1 aaggagcaag aaggaaatcc agaaattaag tctaaacgca gagagcgcat caggaaattc
       61 tttctgagca attgaagtct gatgtcagta atagtcgttt gatgatttcc aaccctactc
      121 acattgcaat agggatatac tttaagccac atctttcacc tattccattg atttctgtaa
      181 gagtaactaa tgaggtggcc ttaggcgtaa ggaaatatgc aaaggaaatc ggaataccaa
      241 ttatcacaga taaaaaatta gcacgaaaaa attatgctac ccatcgtcgc tacgattatg
      301 ttagcttcga aaatatagat gaaatattac gtcttgtgct gtgggttgaa gatgtggaga
      361 atgctgggca acctgttcaa gatgaacagc tctcttcaga agataaattc attgagggtg
      421 aagacacaaa aatcgagaat aaagacgata atttaaaaaa ttaaacatct gtcgtagtca
      481 gaaatttata aggatctaca cgcaaaaagc gtgtagatcc ttatttaatg agttaaacga
      541 tcttgtgaaa tgataagcaa ttgatacata ctcatctctt tataagctgg aactgtagta
      601 acctgtgcaa attcgcgata tagcttttgc tccagttctg ctgatgatat atattccggg
      661 tgttcgctaa agaactgtct tgcatctact atcgcttggg attcgccact tcttttattt
      721 tgattctcct ttaatgaatt ttctctggtg catgcagaga gcaacgatat cagcgcgatg
      781 gttattatga tgttactatt atatcctact aatgacatat caactttctc ttacgcaaga
      841 ttggagttta aaataatgtt tatcc
//
LOCUS       DAZFVO020000423          861 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFVO020000423 DAZFVO020000000
VERSION     DAZFVO020000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.430"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            5..>861
                     /gene="yfdF"
                     /locus_tag="ACHKNB_RS26635"
                     /old_locus_tag="pgaptmp_005327"
     CDS             5..>861
                     /gene="yfdF"
                     /locus_tag="ACHKNB_RS26635"
                     /old_locus_tag="pgaptmp_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HGU4198634.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDA"
BASE COUNT          322 a          157 c          158 g          224 t
ORIGIN      
        1 aatcatgcta ccatctattt caatcaacaa taccagcgca acttacccag aatccattaa
       61 tgaaaataac aatgatgaag ttaatggatt agtacaagag ctcaaaaacc tttttaatgg
      121 taaggaagga ataagcacct gtattaaaca tctacttgag cttataaaaa acgccatacg
      181 agtaaacgac gatccttata gatttaacat taataattcc tcagttactt atattgatat
      241 tggctccaat gatacagacc atattactat tggtatcgac aaccaagaac caatagaatt
      301 acctgcgaac tataaagaca aagaactcgt ccgtactatc attaatgaca acatagttga
      361 gaagactcat gatatcaata acaaggaaat gatcttcagc gcattaaaag aaatatatga
      421 tggagatcct ggttttatct tcgataagat atcacacaaa ctcagacata cggtaacgga
      481 atttgatgag agcgggaata gcgaaccaac ggacttattt acctggtacg gtaaagataa
      541 aaaaggcgac tctctcgcta ttgtaattaa aaataaaaac ggaaatgatt acttatctct
      601 cggttactac gatcaggacg actaccacat tcaaagagga attcgtatta atggtgatag
      661 tctcacccaa tattgtagtg aaaacgccag gagtgcttca gcgtggtttg aaagcagcaa
      721 agctatcatg gcagaatcat ttgcaacagg ttccgatcat caggttgtaa acgagctcaa
      781 cggggaaaga ctgagagaac ctaacgaagt ttttaaacgt ttaggtcgag caataagata
      841 taattttcaa gtggacgatg c
//
LOCUS       DAZFVO020000424          848 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFVO020000424 DAZFVO020000000
VERSION     DAZFVO020000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.431"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>848
                     /locus_tag="ACHKNB_RS26640"
                     /old_locus_tag="pgaptmp_005328"
     CDS             <1..>848
                     /locus_tag="ACHKNB_RS26640"
                     /old_locus_tag="pgaptmp_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HGU4198635.1"
                     /translation="EGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIEGHPAPAAGF
                     VMASPENFRFSEERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDESTGEPVELRMLA
                     ITGDPGLTGMNPVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPE
                     LGTAALSALTGIKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNA
                     LRTEYVALSAQHGSTTLEQLLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQ
                     PVAALTG"
BASE COUNT          184 a          247 c          250 g          167 t
ORIGIN      
        1 gagggctggc tgatcaacca tgcagccgtt gagcgcatgg tctcccgtgt tgtggcgctc
       61 aatcagccgg tgaaaattga ctacaaccac cagaccctga ttgagggaca tccggcaccg
      121 gctgccggtt ttgtgatggc ctcaccggag aattttcgct tcagtgaaga gcgaggtttc
      181 gaggtgcgcc cgaagtggaa ccctccggcc cttgaacatc tgcgtaataa cgaattcccc
      241 tggttttcac cggtgattgg ctatgacgag agcaccggcg aacctgtcga acttcggatg
      301 ctggctatta ccggtgaccc cggtctgacc ggcatgaatc ctgtcgccgc actgtcggcg
      361 gatgaccttt ataacgcctt aaaccctcct ttaaaggata cctccatgaa tgagcaatta
      421 cgccagttgc tgacggcgct cggtctgacc gtggctgacg gtgacgaatt tacgccggag
      481 ctgggcacgg cagcgctgtc tgccctcacc gggatcaaaa cccgtgcgga tgcacacgac
      541 aacctgaaaa cacaggtcgc cagtctgtcg gcagagctgg aaactgcaaa aggcacaccg
      601 gctggcggca gtattgacct gacgaaatac gtgcccgttg aaacgtataa cgccctgcgt
      661 accgaatacg ttgcgctgtc ggcgcagcac ggcagcacca cgctggagca gttgctggac
      721 aaggccgagt ccgaaggacg catcttcaaa agtgaacgcg gctatctgga acaactgggc
      781 gggcagattg gcgtggcggc actttctgca cagcttgacg cccgccagcc ggttgcggcc
      841 ctgaccgg
//
LOCUS       DAZFVO020000425          836 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFVO020000425 DAZFVO020000000
VERSION     DAZFVO020000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.432"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..430
                     /locus_tag="ACHKNB_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /pseudo
     CDS             1..430
                     /locus_tag="ACHKNB_RS26645"
                     /old_locus_tag="pgaptmp_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            463..723
                     /locus_tag="ACHKNB_RS26650"
                     /old_locus_tag="pgaptmp_005330"
     CDS             463..723
                     /locus_tag="ACHKNB_RS26650"
                     /old_locus_tag="pgaptmp_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198636.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDKGL"
BASE COUNT          237 a          168 c          227 g          204 t
ORIGIN      
        1 gccgtccgct gatgctcttt aacagtgaag gtaaaaccgt ctggcgaccg ggcagaccag
       61 cctgtggggg ctggcactca gcctgcccgc agacaccggc tacccggacc cgcgcgggga
      121 actggacccg gaagccgacc ccggcctgct gtatgcgggg cagtggcggg atgcggaatc
      181 ggggctgtgc tataaccggt tccggtatta cgagccggag agcgggatgt acctggtgag
      241 tgatccactg gggttgcagg gaggggagca gacttaccgg tatgtgccga atcctttaag
      301 atggatagat cccttaggat taaataaagg agcttcatta tctaaaatga tgaatagctc
      361 cagtgatctc atggggttga gaaggcagcc ccagaacttc tggcggctat atcgcggaaa
      421 agacatttaa taatagcatt accaggctca gatgaattca gaatgcttga acattttgga
      481 gccgaagctt cggtattaga catgacgatt attgttcgtt caaatccgag taaagccgct
      541 attcttgaag agtttttaca tggtacacaa gagaaactag gaatagctga aaaattagga
      601 cgttatggac tagggagtgc agaaacgcac gttaaggatt tcatgataag acacaaaaaa
      661 atgcttgggt tatctgatga ggatgtagca atactcaaaa tattgaaaga caaggggttg
      721 tgatgaatat aattctgccg ataaaagata tatttattat tgcgggaaac atagttttta
      781 gcgttttata caatgaacat cttgagttcc catgccgctg ccaattacta tcttca
//
LOCUS       DAZFVO020000426          834 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFVO020000426 DAZFVO020000000
VERSION     DAZFVO020000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.433"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..68)
                     /locus_tag="ACHKNB_RS26655"
                     /old_locus_tag="pgaptmp_005331"
     CDS             complement(<1..68)
                     /locus_tag="ACHKNB_RS26655"
                     /old_locus_tag="pgaptmp_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4198637.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVL"
     gene            complement(68..397)
                     /locus_tag="ACHKNB_RS26660"
                     /old_locus_tag="pgaptmp_005332"
     CDS             complement(68..397)
                     /locus_tag="ACHKNB_RS26660"
                     /old_locus_tag="pgaptmp_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198638.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(394..>834)
                     /locus_tag="ACHKNB_RS26665"
                     /old_locus_tag="pgaptmp_005333"
     CDS             complement(394..>834)
                     /locus_tag="ACHKNB_RS26665"
                     /old_locus_tag="pgaptmp_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198639.1"
                     /translation="IGGAVGGGASASGGTAIQAAAANFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVI
                     QNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          171 a          310 c          180 g          173 t
ORIGIN      
        1 agcaccaccc gcggtgactg ctctgactta accgactcgt tcaggcttgc ttcgtgaata
       61 tcctgcatca gttcaccacc tgcttaaact ccgcgctgaa ctcaacgcgc aacatcccga
      121 cccgcgcaga ccacccggca caggtcacct ttatctgccg gtatgcatag ggtggcttcc
      181 acaaaaatgc cttccagccc ccgtgctctg ccaggaacgc ttccagatgc cgggcctcct
      241 cccgggtcac ggaaagcgtc accctgtatg ttttcaggtc agcattcagc cctgccgcca
      301 tacgctgtga gtacccgtca ccaaaacgca cttcacgcac cgacggctgc gagttcacct
      361 ccatatccgg cttcactttc cagcgaaagg ttttcatcgc ccgctccctg ataacatacc
      421 gccatcacgc aactgcagcc ggagttcatc ctgcgccccc ttgcgggcca tgtcatacac
      481 cgccttcatc agctgcggcc ccgcctgtcc gttgataccg tcgttctgaa tcaccacgtg
      541 attgttctga ttaaaattaa taccttccgc ccgccgcatc tgcgccggac ttccggcacc
      601 acccacataa ccaccttccg catagccgcg catcagacgg taaagattcc ccacacctat
      661 ccggctggtt gcctctttcg tgaaaacaaa ctccccgcgg tgaactatcc ccgcaggctc
      721 atatttgccg cccgtgcccg taaatccgcc ggtcgcaaaa tgaaagttcg ccgcagcggc
      781 ctgaatggct gtaccgcctg acgcggatgc gccgccacca acagccccgc caat
//
LOCUS       DAZFVO020000427          825 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFVO020000427 DAZFVO020000000
VERSION     DAZFVO020000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.434"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(30..239)
                     /locus_tag="ACHKNB_RS26670"
                     /old_locus_tag="pgaptmp_005334"
     CDS             complement(30..239)
                     /locus_tag="ACHKNB_RS26670"
                     /old_locus_tag="pgaptmp_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HGU4198640.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEHALSFAAQS"
     gene            complement(335..709)
                     /locus_tag="ACHKNB_RS26675"
                     /old_locus_tag="pgaptmp_005335"
     CDS             complement(335..709)
                     /locus_tag="ACHKNB_RS26675"
                     /old_locus_tag="pgaptmp_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4198641.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESVRKK"
     gene            complement(724..>825)
                     /locus_tag="ACHKNB_RS26680"
                     /old_locus_tag="pgaptmp_005336"
     CDS             complement(724..>825)
                     /locus_tag="ACHKNB_RS26680"
                     /old_locus_tag="pgaptmp_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198642.1"
                     /translation="DAGKYTCVVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT          175 a          290 c          183 g          177 t
ORIGIN      
        1 aaaaaaaccg cctcagcggt tctcatcatt catgactgtg ctgcaaagct cagcgcgtgt
       61 tccagcgccg caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg
      121 gcgtccttca gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca
      181 ttacggatat ccccgcgctc atcacccagc ggggatatcc tgtcgaactc catccacatc
      241 atcgcctcgc tcgcactcag gctgtgccgc agttcggata aggtgcgccc cagacggagc
      301 gcaagtcgca tcagaaagcg aatttccggg cgggctactt ttttctgacc gactctgcat
      361 cagcgatcag ttccagtgcc tgacgcagca agcgggcatg taccggacca tagacggcca
      421 gcacctgctc acggtcgtcc ggagtgaaca cccgttgcag atccgtatca cacaggacat
      481 cgcagaacag cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg
      541 tatcatcatc ctctccatca ccattgagca cgtcctgcca cagataccag gcctctgccg
      601 aaggctcccg cagcaccacg ctgacattac cccattccgg caccttcacc gttttatgac
      661 gaaaccctga cagtctggcc agcgccagcg ttttcagatc ctttttcatg atgacccatc
      721 cccttatccg gcggctgcgc tcactgtcac ggtgcattca acagacgtca cactctgtgc
      781 tttctctgcc gaatcagtca ccacgcaggt atatttcccc gcatc
//
LOCUS       DAZFVO020000428          822 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFVO020000428 DAZFVO020000000
VERSION     DAZFVO020000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.435"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            97..813
                     /locus_tag="ACHKNB_RS26685"
                     /old_locus_tag="pgaptmp_005337"
     CDS             97..813
                     /locus_tag="ACHKNB_RS26685"
                     /old_locus_tag="pgaptmp_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGU4198643.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          272 a          141 c          174 g          235 t
ORIGIN      
        1 tagacgagat ttttcctgtt atgatataat atgccgaatt aacacatgtt aacgatttac
       61 cagtaatgta aataaatttt cgaggagatc attccagtgg gacgtaaatg ggccaatatt
      121 gttgctaaaa aaacggctaa agacggtgca acgtctaaaa tttatgcaaa attcggtgta
      181 gaaatctatg ctgctgctaa acaaggtgaa cccgatccag aattaaacac atctttaaaa
      241 ttcgttattg aacgtgcaaa gcaggcacaa gttccaaagc acgttattga taaagcaatt
      301 gataaagcca aaggcggcgg agatgaaacg ttcgtgcagg gacgttatga aggctttggt
      361 cctaatggct caatgattat cgccgagaca ttgacttcaa atgttaaccg tacgattgct
      421 aacgttcgca caattttcaa taaaaaaggc ggcaatatcg gagcggcagg ttctgtcagc
      481 tatatgtttg acaatacggg tgtgattgta tttaaaggat cagaccctga ccatattttt
      541 gaaattttac ttgaagctga agttgatgtt cgtgatgtga ctgaagaaga aggtaacatt
      601 gttatttata ccgaacctac tgaccttcat aaaggaatcg cggctctaaa agcagctgga
      661 atcactgagt tctcaacaac agaattagaa atgattgctc aatctgaagt tgagctttcc
      721 ccagaagatt tagaaatctt tgaagggctt gttgatgccc ttgaagatga cgacgatgta
      781 caaaaagttt atcataacgt cgcaaatctc taattatatt tt
//
LOCUS       DAZFVO020000429          820 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFVO020000429 DAZFVO020000000
VERSION     DAZFVO020000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.436"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            353..>820
                     /gene="kgtP"
                     /locus_tag="ACHKNB_RS26690"
                     /old_locus_tag="pgaptmp_005338"
     CDS             353..>820
                     /gene="kgtP"
                     /locus_tag="ACHKNB_RS26690"
                     /old_locus_tag="pgaptmp_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HGU4198644.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEG"
BASE COUNT          210 a          199 c          191 g          220 t
ORIGIN      
        1 tccccatgcg agagtaggga actgccaggc atcaaattaa gcgaaaggcc atccgtcagg
       61 atggcctttt tgcgttggtg aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
      121 acatatgcca gattcagcag cgaatacggc ttccccaact tgcccacctc catacgtgtt
      181 ctcctcacca gaaatttatc cttaagctcc tcaataacca ttttcctgct aactaaattc
      241 atggttaagg ttgcataatg atacgcaaca aatgtataac attttcttta ccaaaagaaa
      301 taaacaaaag cgaccgacaa aagcatcgga ttacggcagg agacataatg gcatggctga
      361 aagtactgta acggcagaca gcaaactgac aagtagtgat acacgtcgcc gcatttgggc
      421 gattgtgggg gcctcttcag gtaatctggt cgagtggttc gatttctatg tctactcgtt
      481 ctgttcactc tacttcgccc acattttctt cccttccggg aacacgacga ctcaactact
      541 acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc ccaataggcg gttggctatt
      601 tggccgcata gccgataaac atggtcgcaa aaaatcgatg ctgttatcgg tgtgtatgat
      661 gtgtttcgga tcgctggtta tcgcctgcct cccaggttat gaaactatag gtacgtgggc
      721 tccggcatta ttgcttctcg ctcgtttatt tcagggatta tccgttggcg gagaatatgg
      781 caccagcgcc acctatatga gtgaagttgc cgttgaaggg
//
LOCUS       DAZFVO020000430          816 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFVO020000430 DAZFVO020000000
VERSION     DAZFVO020000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.437"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>816
                     /locus_tag="ACHKNB_RS26695"
                     /old_locus_tag="pgaptmp_005339"
     CDS             <1..>816
                     /locus_tag="ACHKNB_RS26695"
                     /old_locus_tag="pgaptmp_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HGU4198645.1"
                     /translation="PSGRPDATAIALLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDK
                     LQRSYQAFAEQFGFGKRETADALKRLRDAGFITLDLRTVEMLDGVKCSNILFVGINPQ
                     AIAAITTPSSVSPESNSNNAISDTAITLKRNTPRRHNGTGDTPNVDTNTEITTEITTE
                     TKNTIDASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARLNEGVN
                     PETMLEGVKRYAGWVSAMGNSGTQFVKQAVTFFGPDRHFEESWEVPAVSAARR"
BASE COUNT          219 a          198 c          225 g          174 t
ORIGIN      
        1 acccagtggg cgtccggatg cgacggctat cgctctgctt tcagagatcg tttactggta
       61 ccgcccgaca gaggtcaggg atgagcacac cggagcgttg ctgggatatc gcaagcgttt
      121 tcagggcgac aaactgcaaa gaagctacca ggcgtttgct gagcagtttg gtttcgggaa
      181 aagggaaacc gcagatgcgc tgaagcgtct gcgcgatgca gggtttatta ctctggattt
      241 acgcacggtg gaaatgctcg atggggtgaa atgcagcaat attttgtttg tcgggatcaa
      301 cccacaggca attgcggcca tcaccacacc ttcttctgtt tcgccagaaa gtaacagcaa
      361 taatgcaatc agcgatacag ctattacgtt aaaacggaac accccccgac gtcataacgg
      421 aacaggggat acgccgaatg ttgatacaaa tacagagatt actacagaga ttacaacgga
      481 gactaaaaac actattgatg catccgctga cgcgtctgcg ccagcgcgtt ctgcccgaca
      541 ggaatattca ccggaatttg aacaggcctg gcaggaatat cccaaacgtg ctggtggcaa
      601 ttcaaaatca gcagccttca aagcctggaa agcccgtttg aacgaggggg taaaccccga
      661 aaccatgctg gaaggtgtga aacgctacgc gggctgggta tctgcgatgg gcaatagcgg
      721 cacacaattt gtgaaacagg ctgtcacgtt ctttggtccg gatcgtcatt tcgaagaatc
      781 ctgggaagtt cctgcggtat ctgcagccag acgcga
//
LOCUS       DAZFVO020000431          814 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFVO020000431 DAZFVO020000000
VERSION     DAZFVO020000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.438"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(179..>814)
                     /locus_tag="ACHKNB_RS26700"
                     /old_locus_tag="pgaptmp_005340"
     CDS             complement(179..>814)
                     /locus_tag="ACHKNB_RS26700"
                     /old_locus_tag="pgaptmp_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4198646.1"
                     /translation="EPDVAGAMYLKSEDGQDWYECQALFSPETLKVVYDSRGVITGYG
                     KDTALLWPVNQSVAEVPDTPENRKIDLSGRWGFDGEKITDLLTAEKARGMKGDEINAW
                     RNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDAENNDVEVTA
                     EELIALSEAAGQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT          171 a          277 c          144 g          222 t
ORIGIN      
        1 taaataaaaa actgatctca tgccgctgga tatttcacat ctgcttacac tgtaatttct
       61 gagcaacatc acaatatcat caaaatttaa ctgtttagtt cagccatgat atttccctgc
      121 gtaattatta cgactctttc agtaaaaaca gaaagggaca atccctcttt tccaccaatt
      181 actcctgtgc ccagccaacc ctgtatgcca gtatctcatc cgcactggtc agcttttcca
      241 gctctttctt catggtgcgc tggcgaacgt ggatttccat ccctttggtg aacatcgcct
      301 gccctgccgc ttcactcagc gctataagct cttctgctgt cacctccaca tcattgtttt
      361 ccgcatccgt ccagaaaaac gcctccggaa gtttccccgc tttcgctgcc gccaccgacg
      421 gctcaagacg cgtctgcgtt gacttcccgt agtcccattt acgcccattg tgctcaaacg
      481 tgtagttcgc cgcttccatc gcattacgcc acgcgtttat ttcatcgccc ttcatccctc
      541 gcgctttctc cgcggtcagc aggtccgtga ttttctcccc gtcaaaaccc cagcgcccgc
      601 tcaggtcaat tttcctgttc tccggggtat ccggcacttc tgccacactc tggttcaccg
      661 gccacagtaa cgcagtgtcc ttaccgtatc ccgtaatcac accccggctg tcatacacca
      721 ccttcagcgt ctccggcgaa aacaacgcct gacactcata ccagtcctga ccatcctcag
      781 acttcaggta catcgcgcct gcaacatccg gctc
//
LOCUS       DAZFVO020000432          811 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFVO020000432 DAZFVO020000000
VERSION     DAZFVO020000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.439"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            14..289
                     /gene="yddL"
                     /locus_tag="ACHKNB_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /pseudo
     CDS             14..289
                     /gene="yddL"
                     /locus_tag="ACHKNB_RS26705"
                     /old_locus_tag="pgaptmp_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            328..>811
                     /locus_tag="ACHKNB_RS26710"
                     /old_locus_tag="pgaptmp_005342"
     CDS             328..>811
                     /locus_tag="ACHKNB_RS26710"
                     /old_locus_tag="pgaptmp_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HGU4198647.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     N"
BASE COUNT          292 a          160 c          135 g          224 t
ORIGIN      
        1 aggattctgt taaatgaaat taaaaatagt tgcggtggtt gtaactggtt tgttagctgc
       61 gaacgtagca cacgctgccg aagtctataa caaggatggt aataaactcg acctttatgg
      121 caaggttacc gctctacgtt attttactga tgataagcgt gacgatggtg ataaaactta
      181 tgcccgtctc ggctttaaag gagaaacgca aatcaatgat caaatgattg gttttggtca
      241 ctgggaatat gattttaaag gctataacga tgaagccaac ggctcgcgcg gcaagaatct
      301 ctgattgagt taagtgccca tcacaatatg atcactgacc ttatattaca caatcatccc
      361 aggatgaaaa caatcacttt aaacgacaac catattgcac atttaaacgc caaaaacact
      421 acaaaactgg aatatttaaa cttaagcaat aacaatttac tgccaaccaa tgacattgat
      481 caactaatat catcaaaaca tctttggcat gtattagtta acgtcatcaa caatgatcca
      541 cttgcacaaa tgcagtactg gactgcagta agaaatataa ttgatgacac taatgaagtg
      601 accattgatt tatcaggact taatttaacc actcaaccac cagggctgca aaacttcacc
      661 tctatcaatc ttgataataa ccaactcaca cattttaatg caaccaacta cgatagactc
      721 gtaaagctaa gtctgaatag taatactctt gagtcaataa attttcctca aggcagaaat
      781 gtaagtatta cacatatatc tatgaataat a
//
LOCUS       DAZFVO020000433          810 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFVO020000433 DAZFVO020000000
VERSION     DAZFVO020000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.440"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..263)
                     /gene="ybfB"
                     /locus_tag="ACHKNB_RS26715"
                     /old_locus_tag="pgaptmp_005343"
     CDS             complement(<1..263)
                     /gene="ybfB"
                     /locus_tag="ACHKNB_RS26715"
                     /old_locus_tag="pgaptmp_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HGU4198648.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMVIIIWL"
     gene            complement(263..>810)
                     /locus_tag="ACHKNB_RS26720"
                     /old_locus_tag="pgaptmp_005344"
     CDS             complement(263..>810)
                     /locus_tag="ACHKNB_RS26720"
                     /old_locus_tag="pgaptmp_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4198649.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMG
                     GTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEA
                     AGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETC
                     KMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          256 a          188 c          121 g          245 t
ORIGIN      
        1 agccatatta ttatcaccat aatatactta ctatcaatcc ttacgccaat aatgtctgat
       61 acagtcccta taaatattag agcaataaag aatactatgc ctgttggaaa acatattacc
      121 atcattccat aagacatgag actaattaac tcgctcacat cacgagtgga gtctaatagt
      181 gaaagtctat gcatggaaaa gaatagtatt aaaagcgata aagccagcca tatagctctg
      241 aataaaaaaa taatatattt cattacatca ctccggtgaa tacaaatcta ataaaacgtc
      301 caatgttatt tccggagcac ttcatcttac atgtttctaa agaatcatta gaccagtctt
      361 ggcagttttt aaatagccct aaatttatag gtgtgaatat atcaggcaat acagaataac
      421 gcggccttac ctctttaaac ttccctttta aacagttctc cacacaacta ttatcggttt
      481 caacgaggtc acaacggcca gcagcttctt ttgtatcatt acttgcttta cctggccccc
      541 atagaccatc tccttttgat tctccccgtt ggtcttggcc accacaagta tagtcccctt
      601 taccatctgg gacacaaaga tattgatgat agaacggatt cccccatata tctggaccgc
      661 ttctttcacc agttccgccc attgaatgca gtggcttaat gcattttagt gtttctaatc
      721 ctaatggatc tatatttgag atcggattca acggatactg ataaaaattc catcccccct
      781 tcagcccaat cggatcctga gtgatatacc
//
LOCUS       DAZFVO020000434          807 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFVO020000434 DAZFVO020000000
VERSION     DAZFVO020000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.441"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            175..250
                     /locus_tag="ACHKNB_RS26725"
                     /old_locus_tag="pgaptmp_005345"
     tRNA            175..250
                     /locus_tag="ACHKNB_RS26725"
                     /old_locus_tag="pgaptmp_005345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Ile,seq:cat)
BASE COUNT          240 a          153 c          181 g          233 t
ORIGIN      
        1 taacatttgc ttgttgactg cgagtatcga ctttgcgaat ggaagcggtg tcgggtttta
       61 tgtcgagtgt aatgacgagc aaggatgaat gagaacgcat aaaaatctgt tatttacatc
      121 atatgatcat catgtttatt gcgtggttta taagtattgt tattgcacac tcaaggccct
      181 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca
      241 agggccacca aatcattatt tacgtatcag gaagagttaa gggtacaagg ggtactcgat
      301 ggcggtctat atttcatgac taacgccatt tcaaggaaga agtgcggtgg gtggtttcga
      361 atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc
      421 attcaaaagt acctggaaaa aatgcctgct atgccaagcg ggcaaattgc ttacatgtgg
      481 aattaagtaa ttaacgataa agccagagta ttatcattta ttatacccct taatttaatc
      541 aggttaatta ctcaaatcac tgaactattc tctgttcgct cactgatgat gagtgagtac
      601 cacaaccaat gtatgtagaa caatgccgta gagtaaggat gaaatgtctc tgccacgcct
      661 taaacatcta ctactgacca gaacacccgc cgcaggcaga tgagttttgt gagcgttaca
      721 ggagcgtggc tatgctcatt aaccaacgct attgagatat aaatttctaa cactcgaaaa
      781 acgaatttca ctaataaatt ataacgg
//
LOCUS       DAZFVO020000435          805 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFVO020000435 DAZFVO020000000
VERSION     DAZFVO020000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.442"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>805
                     /locus_tag="ACHKNB_RS26730"
                     /old_locus_tag="pgaptmp_005346"
     CDS             <1..>805
                     /locus_tag="ACHKNB_RS26730"
                     /old_locus_tag="pgaptmp_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198650.1"
                     /translation="TREGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLN
                     MLLRRSLVEVSIPGELLTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAAS
                     AKSASDSAKTATSRAAEAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAE
                     KARQHAEKARLAQESAGEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGP
                     KGETGPVGPQGPAGPKGERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPG"
BASE COUNT          210 a          188 c          288 g          119 t
ORIGIN      
        1 acccgtgaag gtggatatta cggaatgacg atggatccgg gggagtatgc ggtttcggtg
       61 acggtgaaag ggaagactgc tgtctacgga cgtgtgcgta ttgaggggac cgaaagtacg
      121 gtgacgctca atatgctgtt acgccgcagt cttgttgagg tgagcatacc cggagagctg
      181 ctgacagatt tccggcagat acagaataat gtggctgatg accttgctac tattcgtcgc
      241 ctgaatgaag acacggcgac aaaaaacact caggccacac agtcaaaaga aagtgcagca
      301 gccagtgcga agagtgcatc tgacagtgca aagacggcaa ccagcagggc ggctgaagcc
      361 ggacaaaaag cgactgatgc cactgaggcc gcgacccgtg caatcacagc agcggggaat
      421 gcagaggaaa gctcgacccg tgccggagag tctgaaaaag ccgccggagc tgatgcagaa
      481 aaagccagac agcatgctga aaaggccagg ctggcgcagg agagcgccgg agagatcctt
      541 aagcgggcag aggctgccac tgtcagtgct gaagaggcca gacgtatggc tgagaatgca
      601 cgggggcccc gggggcctca gggagaaact ggtccgaagg gggatgtcgg tcctaaaggc
      661 gaaacaggtc cagtgggccc tcaagggccc gcagggccga aaggtgagcg tggtgacgtt
      721 ggtgctcagg gggctgtagg gcccgctggt ccgcgtggtg agaagggcga acagggggag
      781 cgaggaccgc agggaatacc aggcc
//
LOCUS       DAZFVO020000436          804 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFVO020000436 DAZFVO020000000
VERSION     DAZFVO020000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.443"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>804
                     /locus_tag="ACHKNB_RS26735"
                     /old_locus_tag="pgaptmp_005347"
     CDS             <1..>804
                     /locus_tag="ACHKNB_RS26735"
                     /old_locus_tag="pgaptmp_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4198651.1"
                     /translation="VLADLQCTFEEKIAAQAQTFEEKLASLSVVLQKCVTGDDVRPML
                     EQIVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSITPDDVRPMLEQMV
                     KEALSHIPVPRDGRDYDPEVLQKAVNDAVGKIPVPADGKSITPDDVRPMLEQMVKEAV
                     SHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAVSHIP
                     VPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR"
BASE COUNT          193 a          188 c          252 g          171 t
ORIGIN      
        1 gtgctggcgg atttacagtg cacatttgag gagaaaatag ccgcgcaggc acaaacgttt
       61 gaagaaaaac tggcttccct gtctgtggta ttacagaagt gcgtgacggg tgatgatgtg
      121 cgtccgatgc ttgaacagat agtgaaggag gcggtaagcc atattcctgt tccgcgtgac
      181 ggtcgtgact acgatccgga agtactgcag aaggcggtga atgatgcggt cgcaaatatt
      241 ccgcagccgg cagacggtaa aagtatcacc ccggatgatg tgcgtccgat gcttgaacag
      301 atggtgaaag aggcgctaag ccatatccct gttccgcgtg acggtcgtga ctacgatccg
      361 gaagtactgc agaaggcggt gaatgatgcc gtcgggaaaa taccggtacc ggcggacggt
      421 aaaagtatca ccccggatga tgtgcgtccg atgcttgaac agatggtgaa agaggcagtg
      481 agccatattc ctgttccgcg tgacggtcgt gactacgatc cggaagtact gcagaaggcg
      541 gtgaatgatg cggtcgcaaa tattccgcag ccggcagacg gtaaaagtct caccccggat
      601 gatgtgcgtc cgatgcttga acagatggtg aaggaggcgg taagccatat tcctgttccg
      661 cgtgatggtc gtgactatga tcccgatgtt ctgcagaagg ctgttctgga tgcggtgagt
      721 gccctgccgg ctccgcagga cgggcgtgat gccacggcac tggaaatact ccccgccatt
      781 gacgatcaaa aatcctttcc ccgg
//
LOCUS       DAZFVO020000437          797 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFVO020000437 DAZFVO020000000
VERSION     DAZFVO020000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.444"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>797
                     /locus_tag="ACHKNB_RS26740"
                     /old_locus_tag="pgaptmp_005348"
     CDS             <1..>797
                     /locus_tag="ACHKNB_RS26740"
                     /old_locus_tag="pgaptmp_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198652.1"
                     /translation="AGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAA
                     ADAVLNADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATD
                     PELLAVNAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENT
                     ALRETAGMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETV
                     QQDDAAPQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAA"
BASE COUNT          195 a          203 c          258 g          141 t
ORIGIN      
        1 gcagggatgc agccggaaag agccacggca ctggcagcag aagctgtcga taaaaaaatg
       61 ccggatttat tccaggcggg cctgatcacc cattccacag ccagtgcaca ggggcagagt
      121 gcaatggcgg cagcagatgc tgttcttaat gcggattact ctgagctggc gcagtcaccg
      181 aaatttcagc agacgttttt gtccattgac gccgacccgc agcacgcaca gcttactgat
      241 cgccagaaaa tggatctggc aaaagagcgt gttgccgatg aggtgcgcgc gcagctggca
      301 accgatcctg aattgctggc tgtgaatgcc atggcggcaa aactgggtga cgcacaactg
      361 tttaatctgg tgacacgagg cacagcgaag accgttaaaa gcggcattgt cagaaatgcc
      421 acggaacagg gggcgattaa tgcggcgcag ggcggctatt cacgctatca ggaaaacacg
      481 gcattgcgtg agaccgccgg aatggatgtg tcaccgtggg agggcgtggc tgacgcaacg
      541 atcgaaggtg cagcctttgg tgctgcgatg ggggctccat ccggtgcggt tgccggatat
      601 cgtggcagac gtcaggccgc agaagaaacc gccatgcgtg atgctgaaac cgtgcagcag
      661 gacgacgcag ccccgcaacc agaatctgtt gatccggtgg cgcagcagcg tgaatccatg
      721 cagggcatga atcgcgagca gcttctggag cagtatgctg atgcggatat ggcaacagag
      781 ggtgacgcat ccgcagc
//
LOCUS       DAZFVO020000438          792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFVO020000438 DAZFVO020000000
VERSION     DAZFVO020000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.445"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            3..293
                     /locus_tag="ACHKNB_RS26745"
                     /old_locus_tag="pgaptmp_005349"
     CDS             3..293
                     /locus_tag="ACHKNB_RS26745"
                     /old_locus_tag="pgaptmp_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4198653.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRACVPGVKKPRARG"
     gene            296..>792
                     /locus_tag="ACHKNB_RS26750"
                     /old_locus_tag="pgaptmp_005350"
     CDS             296..>792
                     /locus_tag="ACHKNB_RS26750"
                     /old_locus_tag="pgaptmp_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HGU4198654.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANA"
BASE COUNT          183 a          220 c          244 g          145 t
ORIGIN      
        1 caatgagtga tttcattact gaagatcagc gtctggtcat tctgcggtcg ctggcagatt
       61 acaacggtga actgggtgaa tccgtgctcc aggactgtct ggatgattac ggccaccgcg
      121 tgtcccgcga taccgttcac acccacattg cctggcttgc cgagcaggga ctggtgcgca
      181 aacgcgttct gattaacggt tatttcatcg cagagctgac cggccggggg caggatgtgg
      241 cggaaggccg ggcctgcgtt ccgggtgtga agaaaccacg cgcaaggggg taacgatgga
      301 caagccgacg cgtggccgtg tgcgcaaggt ggatttgctc cccgacagca ttcgtaagcc
      361 gctgctggaa atgctgcgtg aaaaacgtct gacgcaggtc cagatccgcg aggaaatcaa
      421 ccgtctgatc cgtgaggcgg gcctgccgga agagcagcag ctttcaccgg cggccatcag
      481 ccgggaagcc tcccgtaatg agctgattgc ccgtaacctg cgtgatttac gcgagcagac
      541 aaaagccatg atggccgaac tgggggacaa gccaaccggt gaaaccacag cgctgattct
      601 ggaaatgtcc cgcgccctga tgtaccgccg cctgcgtgcc gcgaccgaat ctctgaacag
      661 cgacagcgac gtggatatgc gcctcatcaa agacatcctc ctgtcagcac agcgtgcgga
      721 aagtgccgcc gagcgcagca ttaaacgtga aaaagaaatc cgggctgcat ttgctgaaga
      781 gatggcaaac gc
//
LOCUS       DAZFVO020000439          792 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFVO020000439 DAZFVO020000000
VERSION     DAZFVO020000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.446"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            3..293
                     /locus_tag="ACHKNB_RS26755"
                     /old_locus_tag="pgaptmp_005351"
     CDS             3..293
                     /locus_tag="ACHKNB_RS26755"
                     /old_locus_tag="pgaptmp_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HGU4198655.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            296..792
                     /locus_tag="ACHKNB_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /pseudo
     CDS             296..792
                     /locus_tag="ACHKNB_RS26760"
                     /old_locus_tag="pgaptmp_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
BASE COUNT          183 a          216 c          240 g          153 t
ORIGIN      
        1 caatgagtga tttcattact gaagatcagc gtcttgtcat tctgcggtcg ctggcggatt
       61 acaacggcga actgggtgaa tccgtgctcc aggactgtct ggatgattac ggtcaccgcg
      121 tgtcccgcga caccgttcat actcatattg cctggcttgc cgagcagggg ctggtgcgta
      181 agcgcacgct cgttaatggt tatttcatcg ctgaactgac cggccggggt caggatgtgg
      241 cggaaggccg cgccaccgtt ccgggtgtga agaaaccacg cgcaaggggg taacgatgga
      301 taagccaacg cgtggccgtg tacgcaaggt ggacttgctc cccgacagca tccgtaagcc
      361 actgctggaa atgctgcgtg aaaaacgtct gacgcaggtc cagatacgcg aggaaatcaa
      421 ccgtctgatc cgtgaggcgg gcctgccgga agagcagtag ctttcaccgg cggccatcag
      481 ccgggaagcc tcccgtaatg agctgattgc ccgtaacctg cgtgatttac gcgagcagac
      541 aaaagccatg atggccgaac tgggggacaa gccgaccggt gaaaccactg cactgattct
      601 ggaaatgtcc cgcgccctga tgtaccgccg tctgcgtgcc gcaaccgaat ccctgaacag
      661 tgacagcgac gtggatatgc gtcttattaa ggacatcctc ctgtcagcac agcgtgcgga
      721 aagtgccgcc gagcgcagca ttaagcgtga aaaagaaatc cgggccgcat ttgctgaaga
      781 gatggcaaac gc
//
LOCUS       DAZFVO020000440          782 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFVO020000440 DAZFVO020000000
VERSION     DAZFVO020000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.447"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>782
                     /locus_tag="ACHKNB_RS26765"
                     /old_locus_tag="pgaptmp_005353"
     CDS             <1..>782
                     /locus_tag="ACHKNB_RS26765"
                     /old_locus_tag="pgaptmp_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198656.1"
                     /translation="LLSSSLVVGLLSVSSFTRANEYSYSALDVLGKHGSTYTIVYNSD
                     SDTAYVKWKKVPSGVEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVYDREFHNLM
                     NSLVKQDKENTLGKKEGTQTDLVSDADIRNLFGRIAAISEMETNVGLDEYGRVATSAE
                     SIKERVSLQTYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIAKDVEEQKE
                     GLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADG"
BASE COUNT          248 a          131 c          196 g          207 t
ORIGIN      
        1 acttctgtca tcttctctgg ttgtaggtct gctgagtgtt agttctttta cacgtgcaaa
       61 tgaatatagc tacagcgcct tggatgtgtt aggtaaacac ggaagtactt acaccattgt
      121 ttataatagt gattctgata ccgcttatgt taaatggaaa aaagtaccta gcggtgttga
      181 ggggattaaa gatggagata ttgtacaagg attatcaaaa attaataaag atgggactgt
      241 agaagttact accaaggatg gcactagtac atatcgtgtg tatgaccggg agtttcataa
      301 cctaatgaat tcactggtca agcaggataa agaaaacact cttggaaaga aagagggtac
      361 tcaaacagac cttgtatctg atgctgatat tcggaatctt tttggcagaa ttgctgcgat
      421 ctctgaaatg gaaactaatg ttggtctgga tgaatatggg cgagtggcaa ctagtgcaga
      481 gagtattaaa gaacgcgtgt ctttgcagac ttatctggct acagaggctg caaatagcgc
      541 aattggccta gataaaaacg gtaagctaac tctgaatgag acagatattg taacgccgtt
      601 acgtatcaat gacggcgttg tcactatagc gaaagatgtg gaagaacaga aagaagggct
      661 ggcgatccag aagaaaggac tgtctgagct ttcatcccgc acagatatca ttgatgcagc
      721 aacggctgcg aatacgaata aaattgattc ccgaaccacc gttggtgtga atgcggacgg
      781 aa
//
LOCUS       DAZFVO020000441          773 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFVO020000441 DAZFVO020000000
VERSION     DAZFVO020000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.448"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>773
                     /locus_tag="ACHKNB_RS26770"
                     /old_locus_tag="pgaptmp_005354"
     CDS             <1..>773
                     /locus_tag="ACHKNB_RS26770"
                     /old_locus_tag="pgaptmp_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198657.1"
                     /translation="TALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLNAD
                     YSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPQLLAVNA
                     MAAKLGDAQLLNLAMRGTAKTVKSGIVRNATAQGAINAAQGGYSRYQENTALRETAGM
                     DVSPWEGVADATIEGAALGAAMGAPFGAVAGYRGRRQAAEETAMRDAETVQQDDAAPQ
                     PESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAA"
BASE COUNT          185 a          197 c          248 g          143 t
ORIGIN      
        1 acggcactgg cagcagaagc tgtcgataaa aaaatgccgg atttattcca ggcgggcctg
       61 atcacccatt ccacagccag tgcacagggg cagagtgcaa tggcggcagc agatgcagtt
      121 cttaatgcgg attactctga gctggcgcag tcaccgaaat ttcagcagac gtttttgtcc
      181 attgacgccg acccgcagca cgcacagctt actgatcgcc agaaaatgga cctggcaaaa
      241 gagcgtgtgg ctgatgaggt gcgtgcgcaa ctggcaaccg atcctcagtt gctggctgtg
      301 aatgccatgg cggcaaaact gggcgatgct cagttgctta atctggcgat gcggggcaca
      361 gcgaagaccg ttaaaagcgg cattgtcaga aatgccactg cacagggggc gattaatgcg
      421 gcgcagggcg gctattcacg ctatcaggaa aacacggcat tgcgtgagac cgccggaatg
      481 gatgtatcgc catgggaggg cgtggctgac gcaacgatcg aaggtgctgc ccttggtgct
      541 gcgatggggg ctccattcgg tgcagttgcc ggatatcgtg gcagacgtca ggccgcagaa
      601 gaaaccgcca tgcgtgatgc tgaaaccgtg cagcaggacg acgcagcccc gcaaccagaa
      661 tctgttgatc cggtggcgca gcatcgtgaa tccatgcagg ggatgaatcg cgagcagctt
      721 ctggagcagt atgctgatgc ggatatggca acagagggtg acgcatccgc agc
//
LOCUS       DAZFVO020000442          765 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFVO020000442 DAZFVO020000000
VERSION     DAZFVO020000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.449"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            189..>765
                     /locus_tag="ACHKNB_RS26775"
                     /old_locus_tag="pgaptmp_005355"
     CDS             189..>765
                     /locus_tag="ACHKNB_RS26775"
                     /old_locus_tag="pgaptmp_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198658.1"
                     /translation="MSKKFTKTLLSSSLGGLLLGISSGVLAQELPPITDSDLPFYSVP
                     KEADVSSVIYGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTVVNPALKNQVG
                     ELSSRTDIIDAATAANTNKIDSRTTVGVNADG"
BASE COUNT          233 a          132 c          167 g          233 t
ORIGIN      
        1 cggtggtgta gtcgtggttc tgagtgacta caccaagaat aatgtaagac aaatcctaaa
       61 attattaaat taatattttg ggtttatttt cggaatatcc tgaataaagc cgcaattaat
      121 catatttatt tatgagtatg aggtgtagtg gctgatttgt atacagataa tttaacagga
      181 tatccataat gagtaaaaaa ttcacaaaga cacttctgtc atcttctctg ggtgggcttc
      241 tgcttgggat cagctcaggt gttttagctc aggagttacc tcctatcaca gattctgatt
      301 tgccttttta ttcagtgccg aaagaggcag atgtttcgtc tgttatatac ggtagtactt
      361 atcgtattta ctataacaaa tcctctggtt ggggaacaat gcgatatgtt ctgaacggaa
      421 aagatgagac cctttttaat ttcgatcagg agggtaatat tatagtattg agtaaagata
      481 actcaattgc atataccgtt catgagcctg tgctaaaaga cttcgcccgt atggcagcag
      541 gcctcagaac gtctgacaaa gaaaacggta agcatcaggt tgatgaggag gaggtccgcc
      601 ggatcttcaa taaagttaat aatctatcta atactgttgt aaatcctgca ttgaaaaatc
      661 aagttggtga gctttcatcc cgcacagata tcattgatgc agcaacggct gcgaatacga
      721 ataaaattga ttcccgaacc accgttggtg tgaatgcgga cggaa
//
LOCUS       DAZFVO020000443          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFVO020000443 DAZFVO020000000
VERSION     DAZFVO020000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.450"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>763
                     /locus_tag="ACHKNB_RS26780"
                     /old_locus_tag="pgaptmp_005356"
     CDS             <1..>763
                     /locus_tag="ACHKNB_RS26780"
                     /old_locus_tag="pgaptmp_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4198659.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT
                     GPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRY
                     GQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQY"
BASE COUNT          187 a          221 c          233 g          122 t
ORIGIN      
        1 cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg cataaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 accgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc
      601 tgcgcaacgg aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag
      661 ttgctgacct tcactgactg ttccggttat gtaacccgct atgaccatga ccgttttggt
      721 caggtgacgg cggtgcaccg cgaggaaggc ctgagccagt acc
//
LOCUS       DAZFVO020000444          763 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFVO020000444 DAZFVO020000000
VERSION     DAZFVO020000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.451"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>763
                     /locus_tag="ACHKNB_RS26785"
                     /old_locus_tag="pgaptmp_005357"
     CDS             <1..>763
                     /locus_tag="ACHKNB_RS26785"
                     /old_locus_tag="pgaptmp_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HGU4198660.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEICYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDHITITDSLNRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSAT
                     GPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRY
                     GQLLSFTDCSGYQTRYDHDRFGQMTAVHREEGLSQY"
BASE COUNT          189 a          219 c          235 g          120 t
ORIGIN      
        1 cgcggcgaac tggcggcggt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatctgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagttac
      181 acgtatcagt atgagaaaga ccacatcacc atcaccgaca gcctgaaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgatgcggtg ggcaggctta aggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggac tcatcacgcg catcaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 actgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacttaccc
      601 tgcgcaacgg aagatgccac cggcagccgg aaaaccatga cgtggagccg ttacggccag
      661 ttgctgagct tcaccgactg ttccggctac cagacccgtt atgaccatga ccgcttcggg
      721 cagatgacgg cggtgcaccg cgaggaaggg ctgagccagt acc
//
LOCUS       DAZFVO020000445          754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFVO020000445 DAZFVO020000000
VERSION     DAZFVO020000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.452"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            176..457
                     /locus_tag="ACHKNB_RS26790"
                     /old_locus_tag="pgaptmp_005358"
     CDS             176..457
                     /locus_tag="ACHKNB_RS26790"
                     /old_locus_tag="pgaptmp_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198661.1"
                     /translation="MTEAEILGLIRRVAGISQQADEQATQPDSMTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
BASE COUNT          217 a          158 c          185 g          194 t
ORIGIN      
        1 gcgcattttt gtgattttat cgtcatcatc atcatgtgtg taacctgttg tttttaatgt
       61 ggttgattca aaaaagatga tgattgtggt taatttttgt tcgacatctt ttagcgtgac
      121 agattcttta caaaaaatct gagcttgttt tcttcaccag cgcgatgggg gcacaatgac
      181 agaagccgaa atactgggat taatccgccg cgtcgccgga atcagccagc aggctgacga
      241 acaggccacg cagccggaca gcatgaccgc cgaaaattat gcgcgtgttg ttgctgaggt
      301 gatgcgccgt gatggtatcc agcttaatga tgcggatatg cgcgacatac ggatccgcgt
      361 tctggaaatg ctggcctaca atcgccgcgt tgaactgtat cgggagaaag aaaaaattac
      421 gtaccactgg aaaaagccgg agcggttacg gcgttaacga gttgtgacat accgtgacgg
      481 agagtgacaa ccagtgacac gacgtgacaa aaagacggta tttaaacagg gggagtattt
      541 aatacccctc ccaccagcct gattaactgt taaccggaaa aaaaatccgg tagggttctc
      601 gatatgggga tccccatatc ggggaacatc caccagcttc ataacggata gccggaaaat
      661 gataacggat aactacgaaa ttttcgcagt ttaataaacc tctgggaaaa ctcgccttta
      721 aattgtacac tttttcagca aatgatttta gtta
//
LOCUS       DAZFVO020000446          754 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFVO020000446 DAZFVO020000000
VERSION     DAZFVO020000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.453"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..594
                     /locus_tag="ACHKNB_RS26795"
                     /old_locus_tag="pgaptmp_005359"
     CDS             <1..594
                     /locus_tag="ACHKNB_RS26795"
                     /old_locus_tag="pgaptmp_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198662.1"
                     /translation="LFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNV
                     VIAENCTINGTLKAENIIGDLVKCAGGAFPVDGSYLANGTRTLTVYDDHSFDRQIIIP
                     PIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGHGHIT
                     LSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          189 a          183 c          219 g          163 t
ORIGIN      
        1 ttgtttgcgg tgagcatcac ctcgtccggc aatcccccga cgttctccct gacgccggag
       61 ggcaggctga cggcccgcaa tgcggacatc agcggacata tcagtgcgaa ctcgggcacg
      121 ctcaataatg tcgtgatagc ggagaactgt acgataaatg gcacgctgaa agcggagaac
      181 attattggtg atcttgtgaa atgtgcaggg ggggcttttc cggtggatgg tagttacctt
      241 gcgaacggta cacgaacgct gacggtgtat gacgatcaca gctttgaccg gcagattata
      301 atcccgccga taatctatgt cgggtcaaaa caggaatccc gtaccagtaa tgacatctgg
      361 acagagtgct tcctgcatgt tgatcagaac ggacgccgga tttattccgg caggtcagtg
      421 acagagccgg gaattttcag cgggatcatc gatatgccag ccggtcatgg tcatatcacc
      481 ctgagtttta ccgtaagttc acgacgtcag aacgggagtt ttggcagttc acgtatcagt
      541 aaccttcagg cgatagtggt gaagaaaaac agcgcgggga tcagcatccg ctgaacatcg
      601 cgccccggga ttgtcggaag gagacaaaaa ccgtacagta tgcgcgggtg cctttggctg
      661 atggccggag ggaacacctg aaggccggat gtgaaaaggc cccggacaaa cattcatgtt
      721 taacccgagg cctgactttc gatcttcagc aaat
//
LOCUS       DAZFVO020000447          751 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFVO020000447 DAZFVO020000000
VERSION     DAZFVO020000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.454"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            394..627
                     /locus_tag="ACHKNB_RS26800"
                     /old_locus_tag="pgaptmp_005360"
     CDS             394..627
                     /locus_tag="ACHKNB_RS26800"
                     /old_locus_tag="pgaptmp_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4198663.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            685..>751
                     /locus_tag="ACHKNB_RS26805"
                     /old_locus_tag="pgaptmp_005361"
     CDS             685..>751
                     /locus_tag="ACHKNB_RS26805"
                     /old_locus_tag="pgaptmp_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198664.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQW"
BASE COUNT          268 a          174 c          140 g          169 t
ORIGIN      
        1 tccattgttc attccacggc caaaaacaga gaaaggaaac gacagaggcc caaaagctcg
       61 ttttcagcac ctgtcgtttc ctttcttttc agggggtatt ttaaataaaa acattaagtt
      121 acaacgaaga agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg
      181 cgaggtcgcc gccccgtaac ctgtcggatc gccggaaagg acccgcaaaa tgataataat
      241 tatcatctgc atgtcacaac gtgcatctac gccatcaaac cacgtcaaac aatcaattat
      301 gacgcagata tcgtattaat tgatccgcat caacttaacg taaaaacaac ttcagacaat
      361 acaaatcagc gacactgaat acggggcaac ctcatgtcaa cgaagaacag aacccgcaga
      421 acaacaaccc gcaacatccg ctttcctaac caaatgattg aacaaattaa catcgctctt
      481 gagcaaaaag ggtccgggaa tttctcagcc tgggtcattg aagcctgccg ccggagactg
      541 tgctcagaaa aaagagtttc ttctgaagca aacaaagaaa agagtgacat tactgaattg
      601 ctcagaaaac aggtcagacc agattgaagc aatttagata atcgtgcaga ctacgccccc
      661 tcatatcaca tggaaggttt atctatggat caggtagtca tttttaaaca aatatttgat
      721 aaagttcgaa acgatttaaa ctatcaatgg t
//
LOCUS       DAZFVO020000448          749 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFVO020000448 DAZFVO020000000
VERSION     DAZFVO020000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.455"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..480
                     /locus_tag="ACHKNB_RS26810"
                     /old_locus_tag="pgaptmp_005362"
     CDS             <1..480
                     /locus_tag="ACHKNB_RS26810"
                     /old_locus_tag="pgaptmp_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HGU4198665.1"
                     /translation="PYGEDVQYFQSEDGRDFYESIPLFTKKYKLCISPVTGIICSVAE
                     DVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKTVAENRRSSLLQEAN
                     GTVDDWKTELKLDMISDENKLKLTRWMAYIRQLKEMHFNDIASEGHYQAIPWPEKPE"
     gene            complement(515..>749)
                     /locus_tag="ACHKNB_RS26815"
                     /old_locus_tag="pgaptmp_005363"
     CDS             complement(515..>749)
                     /locus_tag="ACHKNB_RS26815"
                     /old_locus_tag="pgaptmp_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198666.1"
                     /translation="QATADGRYVRNVQLGAQSRHSPAGNEVTWDYKTPAGCMLSGIHV
                     QDTGSRSADNIGGVYYRPVQIYINNAWRTVSSV"
BASE COUNT          204 a          165 c          187 g          193 t
ORIGIN      
        1 ccctatggtg aggatgttca gtatttccag agtgaggacg gaagggattt ttatgaatct
       61 atcccgcttt tcacaaagaa atacaaactc tgtatttcgc ctgtaacggg cattatctgc
      121 tctgttgctg aagatgtctc tgctttatat ccggcaggtt ttactgttgt tgaagtggac
      181 gaactgccgg aaggtgtgaa tattgacggt aactggcagt tttctgatgg cctgatatca
      241 aaggtgccgg taaactggaa gacggttgct gaaaatcgcc gctcatcgct gctccaggag
      301 gccaacggaa ccgtggatga ctggaaaaca gaattaaaac tggacatgat tagcgatgaa
      361 aataaattaa agctgacgcg ctggatggcg tatatccgac agcttaaaga aatgcacttt
      421 aatgatattg ccagtgaggg gcattaccag gcgattccct ggccggaaaa accggaataa
      481 ttcagagggt gcattcagca cccttttctg tggtttacac tgaactcacc gttctccagg
      541 cattattaat ataaatctga accgggcgat aatagacccc accgatatta tccgcagacc
      601 gggagccggt atcctgtaca tgaataccgg aaagcataca acctgctggc gttttataat
      661 cccaggtgac ttcattaccc gcaggagagt ggcggctctg agcaccaagt tgcacattac
      721 gaacatatct gccgtctgct gtagcctgg
//
LOCUS       DAZFVO020000449          744 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFVO020000449 DAZFVO020000000
VERSION     DAZFVO020000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.456"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(26..214)
                     /gene="bcsR"
                     /locus_tag="ACHKNB_RS26820"
                     /old_locus_tag="pgaptmp_005364"
     CDS             complement(26..214)
                     /gene="bcsR"
                     /locus_tag="ACHKNB_RS26820"
                     /old_locus_tag="pgaptmp_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HGU4198667.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            487..>744
                     /locus_tag="ACHKNB_RS26825"
                     /old_locus_tag="pgaptmp_005365"
     CDS             487..>744
                     /locus_tag="ACHKNB_RS26825"
                     /old_locus_tag="pgaptmp_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
                     /protein_id="HGU4198668.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSML"
BASE COUNT          212 a          164 c          156 g          212 t
ORIGIN      
        1 cccagtacgg ccattcacca atcccctact tttgttgcgc aaactctgcc agcaacggcc
       61 agcgttttaa tgccgcggcc aactgttcgc gttgggaaat atcggcataa tcaatatcag
      121 gcagtgaaaa tgcctgcttt aacgccacaa tatcattctg gaagatatag cctatcgcgg
      181 gatcaggcag agtatctggt tcattgttat tcattttgct tgatccctat gaaataaccg
      241 taccgttcat gagacaattc taaccgtgag catttcgccg gtttattgtt tacggcgaga
      301 cgggaatttg ctcacttttt gactttccgt tacacaacag aaaaagttaa aaacgaaaag
      361 actaaaatca cacgtcttgt gaccttctaa attgattcac atgctaaatc tgataagttt
      421 taatttcaat ggtaggttta tttcttagct ttcgctagta aactgataat cagacaaaat
      481 ggtgacatga gggacattgt ggaccctgta ttctctatcg gtatctcatc attatgggat
      541 gagctgcgac atatgccagc aggcggcgtc tggtggttta acgtcgatcg ccatgaagat
      601 gctatcagtc tggcgaatca aacaattgca tcccaggctg aaaccgcaca cgtcgcggtc
      661 attagcatgg acagcgatcc ggcgaaaatc tttcaattag atgattctca agggccggaa
      721 aaaataaaat tattttcaat gcta
//
LOCUS       DAZFVO020000450          744 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFVO020000450 DAZFVO020000000
VERSION     DAZFVO020000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.457"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..594
                     /locus_tag="ACHKNB_RS26830"
                     /old_locus_tag="pgaptmp_005366"
     CDS             <1..594
                     /locus_tag="ACHKNB_RS26830"
                     /old_locus_tag="pgaptmp_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198669.1"
                     /translation="LFAVSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNV
                     VIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIIIP
                     PIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGIFSGIIDMPAGHGHIT
                     LSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          187 a          184 c          216 g          157 t
ORIGIN      
        1 ctgtttgcag tgagcatcac ctcgtccggc aatcccccga cgttctccct gacgccggag
       61 ggcaggctga cggcccgcaa tgcggacatc agcggacata tcagtgcgaa ctcgggcacg
      121 ctcaataatg tcgtgatagc ggagaactgt acgataaatg gcacgctgaa agcggagaac
      181 attattggtg atcttgtgaa atgtgcaggg gtggcttttc cggtggatgg tagttacctt
      241 gcgaacggta cacgaacgct gacggtgtat gacgatcaca gctttgaccg gcagattata
      301 atcccgccga taatctatgt cgggtcaaaa caggaatccc gcaccagtaa tgacatctgg
      361 acagagtgct tcctgcatgt tgatcagaac ggacgccgga tttattcagg caggtcagtg
      421 acagagccgg gaattttcag cgggatcatc gatatgccag ccggtcatgg tcatatcacc
      481 ctgagtttta ccgtaagttc acgacgtcag aacgggagtt ttggcagttc acgtatcagt
      541 aaccttcagg cgatagtggt gaagaaaaac agcgcgggga tcagtatccg ctgaacatcg
      601 cgccccggga ttgccggaag gagacaaaaa ccgtacagta tgcgcgggtg cctttggctg
      661 atggccggag ggaacacctg aaggccggaa agacgaaggc cccggacaaa cattcatgtt
      721 taacccgagg cctgacgttc ttcc
//
LOCUS       DAZFVO020000451          742 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFVO020000451 DAZFVO020000000
VERSION     DAZFVO020000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.458"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>742
                     /locus_tag="ACHKNB_RS26835"
                     /old_locus_tag="pgaptmp_005367"
     CDS             <1..>742
                     /locus_tag="ACHKNB_RS26835"
                     /old_locus_tag="pgaptmp_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HGU4198670.1"
                     /translation="ITISYEFILCQHLIANTEFSYLALPENYNRLFLPNSKNQTNNRF
                     KTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASI
                     LLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLANLRKEFNKTVR
                     KNFLIKLAKTSETSGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDFENLVLIEN
                     EPYHKVFTNMQSRIAKGILVGESKITPWA"
BASE COUNT          289 a          126 c          125 g          202 t
ORIGIN      
        1 ataactatta gttatgaatt tatcctctgt caacatctta tcgcaaatac cgagttcagc
       61 tatttggcac tccctgaaaa ttataaccgt ttgtttttac caaattcaaa aaaccaaaca
      121 aataatcgtt tcaaaacgtt aaacagcaaa gctattggca ggctacttgc tgctggtggc
      181 gtatacaatg ggaacattga aggattcaga gatactgcgg aaaaactggg tggagatgca
      241 ataaaaggct atgatcaaat actaaatgaa aaaacagcgg gcatagcgat agcaacagca
      301 tctattcttt taacaaagcg ttctaatgtt gatacatata cagaaataaa tagttactta
      361 ggcaaactta gaggtcaaca aaaacttctt gatggtatag acataataga aataatatac
      421 attaagagac cttcaaaaga cttagctaac ttacgaaagg agtttaataa aactgtaaga
      481 aaaaattttc ttatcaaact tgcaaaaacc tccgaaacat ctggaagatt caacgccgaa
      541 gaccttttaa gaatgagaaa gggcaatgtt cctctaaatt ataatgttca ccataaacta
      601 tctctagatg atggtggtac taatgatttc gaaaatttag tattaatcga aaacgaacca
      661 tatcataaag tttttactaa catgcaatca cgaatagcta agggaatatt agtaggtgaa
      721 agcaaaatca ctccctgggc ca
//
LOCUS       DAZFVO020000452          741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFVO020000452 DAZFVO020000000
VERSION     DAZFVO020000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.459"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..>741
                     /locus_tag="ACHKNB_RS26840"
                     /old_locus_tag="pgaptmp_005368"
     CDS             12..>741
                     /locus_tag="ACHKNB_RS26840"
                     /old_locus_tag="pgaptmp_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGU4198671.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDL"
BASE COUNT          231 a          165 c          137 g          208 t
ORIGIN      
        1 cggagtagtg tatggccaat ctctacgact tgaaaaagtt cgacttaaac cttcttgtca
       61 tatttgagtg tatttaccag catttaagca ttagcaaagc tgctgaatca ctatatatca
      121 ccccatcagc cgtgagccag tcgttacaac gcttacgtgc gcaatttaat gaccctttat
      181 tcattcgctc aggaaaaggt atcgcgccga cgacaacagg gttgaatctg catcaccatc
      241 ttgagaaaaa cctcaaaggc cttgaacaaa caattaatat agtcaataag tccgaactaa
      301 agaaaaactt tattatctac ggccctcagc tcatttcttg ctctaataat agtatgctga
      361 tccgctgcct acggcaagat gcctctgtcg agattgagtg ccatgatatc ctcatgtcag
      421 cagagaatgc tgaagaactg ttggttcacc gcaaagccga tctggtgatc acccaaatgc
      481 cagtgatcag ccgttccgtc atttgcatgc cattgcatac aattcgcaat actctaattt
      541 gtagtaataa gcatccgcga attactgaca atagcactta tgaacaaatt atggcagaag
      601 agttcaccca attaatatcc aaatcagcag gagttgatga tattcaaatg gaaatagacg
      661 agaagtttat gaatcgcaaa atttcatttc gtggttcctc tttgctgaca atcattaata
      721 gcattgcagt tacagattta c
//
LOCUS       DAZFVO020000453          741 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFVO020000453 DAZFVO020000000
VERSION     DAZFVO020000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.460"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..412
                     /locus_tag="ACHKNB_RS26845"
                     /old_locus_tag="pgaptmp_005369"
     CDS             <1..412
                     /locus_tag="ACHKNB_RS26845"
                     /old_locus_tag="pgaptmp_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4198672.1"
                     /translation="FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVP
                     LAFGKNLDKTLTVNTGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQS
                     ADAGKYTCVVTDSAEKAQSVTSFECTVTVSAAAG"
     gene            427..>741
                     /locus_tag="ACHKNB_RS26850"
                     /old_locus_tag="pgaptmp_005370"
     CDS             427..>741
                     /locus_tag="ACHKNB_RS26850"
                     /old_locus_tag="pgaptmp_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HGU4198673.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHA"
BASE COUNT          164 a          172 c          249 g          156 t
ORIGIN      
        1 gtttccgtcc ggtaagggct ttaagttcct ggcggaagtg cgtcagcaca cctggtcatc
       61 cggtaccaac ggcgtggtgg cagcaacgtt ttcactgcgt ctgaaaggca aaccggtgtc
      121 ctttgtggta ccgctggcgt ttgggaaaaa tctggataag acacttaccg tgaataccgg
      181 tgcgctgctg acaatgtcag tcagtgccaa cgggggaacg ccgccgtata aatacgcctg
      241 gaagaaggat ggtcagccgg ttgacggtca gacgacagac accttcagta agccaggtgc
      301 gcagtccgct gatgcgggga aatatacctg cgtggtgacc gattcggcag agaaagcaca
      361 gagtgtgacg tcttttgaat gcaccgtgac agtgagcgca gccgccggat aaggggatgg
      421 gtcatcatga aaaaggatct gaaaacgctg gcgctggcca gactgtcagg gtttcgtcat
      481 aaaacggtga aggtgccgga atggggtaat gtcagcgtgg tgctgcggga gccttcggca
      541 gaggcctggt atctgtggca ggaagtgctc aatggtgatg gagaggatga cgataccctg
      601 tcggtggtgg cgaaaacccg ccgtaacctg gaagcggatg tgacgctgtt ctgcgatgtc
      661 ctgtgtgata cggatctgca gcgggtgttc actccggacg accgtgagca ggtgctggcc
      721 gtctatggtc cggtacatgc c
//
LOCUS       DAZFVO020000454          726 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFVO020000454 DAZFVO020000000
VERSION     DAZFVO020000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.461"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            35..>726
                     /locus_tag="ACHKNB_RS26855"
                     /old_locus_tag="pgaptmp_005371"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHKNB_RS26855"
                     /old_locus_tag="pgaptmp_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HGU4198674.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          166 a          186 c          205 g          169 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 cgcccgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZFVO020000455          724 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFVO020000455 DAZFVO020000000
VERSION     DAZFVO020000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.462"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(173..>724)
                     /locus_tag="ACHKNB_RS26860"
                     /old_locus_tag="pgaptmp_005372"
     CDS             complement(173..>724)
                     /locus_tag="ACHKNB_RS26860"
                     /old_locus_tag="pgaptmp_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGU4198675.1"
                     /translation="SADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTLPQQF
                     SEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSLYMSE
                     SLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISVFREY
                     FGITPHQMMINQSGFIESRIAIK"
BASE COUNT          222 a          142 c          151 g          209 t
ORIGIN      
        1 gaccaataca ttctttgctc gcatccgtaa aaatgtcgcc acaatatcat cagtcagtca
       61 actcattacc atctgttttc cggggccgta gttactacag tgctccggag tgataaacag
      121 aaactgataa tgaagcataa gaatactttt attatgcgct gactgacata ctttacttaa
      181 ttgcgattct gctctcaata aatccggact gattaatcat catctgatga ggtgttatgc
      241 caaaatattc tctgaaaacg ctgataaaat acggtgtgct ggcataaccg caccgtattg
      301 cgatttgatt cactgacagt ttactggaaa taagttgtcg ggcgttatgc atgcgtatat
      361 ccagcaggat ttgggagaaa gaaacttttt cctcctgaag ttttcttttc agtaaactct
      421 cgctcatgta cagagaatta ctgatatctt taactctcca tgcccgtgat ggactggaac
      481 agataatata cctgacttta agcgcaacag agtttatgca tctgaatagc agaaacatga
      541 aacctctgtg tgtggaaaaa atggaaaggc aggcaaaaaa catctgctca gaaaattgtt
      601 gtggcaatgt cttttgcaga ctgagattct gcatgacatt atgtaacaga gcgatgtgag
      661 agaaggttgt tttaatgaac ggaacagaaa cagcactatc agagtgctgg atcctgtcgg
      721 cact
//
LOCUS       DAZFVO020000456          708 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFVO020000456 DAZFVO020000000
VERSION     DAZFVO020000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.463"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>708
                     /locus_tag="ACHKNB_RS26865"
                     /old_locus_tag="pgaptmp_005373"
     CDS             <1..>708
                     /locus_tag="ACHKNB_RS26865"
                     /old_locus_tag="pgaptmp_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4198676.1"
                     /translation="LTAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISKGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYT"
BASE COUNT          177 a          210 c          220 g          101 t
ORIGIN      
        1 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acgccgccgg
       61 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      121 gggaaaagcc atctgtacca cgcagggcgg tctgacgcgc agtatggaat acgatgctgc
      181 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      241 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      301 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      361 cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa accgcagagc agtggcagta
      421 tgacgaacgc ggctggctga cagacatcag ccatatcagc aaagggcacc gggtgacggt
      481 gcattacggg tatgatgaga aaggccggct gaccggtgag cgtcagacgg tgcatcaccc
      541 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
      601 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
      661 ttacctggca ggcatgaaac tcggcgacac accgctggtg gagtacac
//
LOCUS       DAZFVO020000457          704 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFVO020000457 DAZFVO020000000
VERSION     DAZFVO020000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.464"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            73..192
                     /locus_tag="ACHKNB_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /pseudo
     CDS             73..192
                     /locus_tag="ACHKNB_RS26870"
                     /old_locus_tag="pgaptmp_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            152..704
                     /locus_tag="ACHKNB_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /pseudo
     CDS             152..704
                     /locus_tag="ACHKNB_RS26875"
                     /old_locus_tag="pgaptmp_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          243 a          109 c          157 g          195 t
ORIGIN      
        1 tcaatgactc tcatgttgaa agagatgtcc aaccttatca atgatgaggg aattaaatag
       61 ttttcataat gagttacagc acaacgttgg gtattgactg agcaagatga acgacaagga
      121 caacgacatc agccttatga ggagcaagaa aatgaaagca aattcgaaaa cgatcagaag
      181 gatgaaagtt aatgttcggt ctgaggaaag taaatcgaaa cactcatacg tttgaaacca
      241 cttttcaaaa ttggaaagaa aatggcgaag atgttgcatt attaatgccc gaatttagtg
      301 ctaaatggtt accgatagca gaagagagtg gcagctggtc tggatgggta ttgcttggag
      361 aaatatttcc cttaatttca tctgagctcg ctgggatggc tttaatgcca gaaacggaaa
      421 ggttaatagg ggaatggctc agtttatcca gttccccgtt aaatctaaag catcctgaac
      481 taaaatataa tcgtttgcac gtaggtaaag tatttgatgg agtattgagc cctgctcaac
      541 cactaataag aatatgaaca ggggaattga atctttggtt agataaagtc acagtctgcc
      601 aatacggaaa cgctccagtg ttagacaaaa aatcattata ttggtccatt cattttgtaa
      661 ttggatttag taaaacatgt tacagaagtc ttgttgatat tgaa
//
LOCUS       DAZFVO020000458          691 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFVO020000458 DAZFVO020000000
VERSION     DAZFVO020000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.466"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..591
                     /locus_tag="ACHKNB_RS26880"
                     /old_locus_tag="pgaptmp_005376"
     CDS             <1..591
                     /locus_tag="ACHKNB_RS26880"
                     /old_locus_tag="pgaptmp_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HGU4198677.1"
                     /translation="VRWPLAELCDYYNDAVRAVILARPDAGASLETLSCVPGARQTLP
                     DGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRGKPECFISNDLSPRVFWLFPV
                     PDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNPLVDWMLFRAFSKDAAGGAES
                     GLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            588..>691
                     /locus_tag="ACHKNB_RS26885"
                     /old_locus_tag="pgaptmp_005377"
     CDS             588..>691
                     /locus_tag="ACHKNB_RS26885"
                     /old_locus_tag="pgaptmp_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198678.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDAL"
BASE COUNT          151 a          153 c          216 g          171 t
ORIGIN      
        1 gtgcgctggc ctctggctga gctgtgtgat tattacaatg atgctgtgcg ggcggtgatt
       61 ctggcaaggc cggatgcagg cgcaagcctg gaaacgctca gttgtgtccc tggcgcacgc
      121 cagactttac ctgatggcgc aatacagctt cttgacgtga tatgcctcag cgatggcagt
      181 gcgattaaac cagtatcccg ggaggtgctt gatgcacagt atcccgactg gcacatgctg
      241 aggggaaaac cggaatgctt tatcagcaac gacctgtccc cgcgcgtatt ctggctgttt
      301 cctgtccctg acaaagagat aagtattgat gcagtggtaa gccggatacc ggaggcagtg
      361 tatgttctga cgcaggacga tgatacgcca gttccactgg aagaggctta tgttaaccca
      421 ctggtggact ggatgttgtt tcgtgctttc agtaaggatg ctgccggtgg tgcagaatcg
      481 gggctggctg cgcagcatta tcagagtttt gttgagcaac ttgggatcaa acagggggca
      541 gacagtgcat tgtctgcccg taaaaaagtg tttaacggag gtggagtgtg agtgttgttg
      601 tttcggggac gctgaaatct cctgatggtg aggcgatatc aggagcaaat attaccctga
      661 cggcgctgac agtttcaccg gatgcgctca g
//
LOCUS       DAZFVO020000459          690 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFVO020000459 DAZFVO020000000
VERSION     DAZFVO020000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.467"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>690
                     /locus_tag="ACHKNB_RS26890"
                     /old_locus_tag="pgaptmp_005378"
     CDS             <1..>690
                     /locus_tag="ACHKNB_RS26890"
                     /old_locus_tag="pgaptmp_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HGU4198679.1"
                     /translation="VLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETI
                     YRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG
                     LYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIA
                     SNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYD
                     FTPESIYRAMTI"
BASE COUNT          244 a          128 c          111 g          207 t
ORIGIN      
        1 gtactgaaag atatgatcat cgaatcagta tggaaaaata ttattgttag tgacgagtcc
       61 ctgacaagat gtatctattc tttgcgctgc atttttgaaa aaattggcta tgatcgttgc
      121 atagaaacaa tctaccggaa aggttatcgt ttcagcgggc aggttttcaa aactaaaatt
      181 aatgaagata atacttcaga ctattccata gctatattcc ctttcactac ttcgttgaat
      241 acactggatc cattaatact taatcaggaa ttagtgcaaa tcatttcaaa taaaaaaatc
      301 gatggtctct atacctatcc gatggctgcg acaaattttt gtaatgatca catatctcaa
      361 aattcattct tgagcagatt caagccagat tatttcgtta caggaagaat aaaccagaat
      421 aatgcagtga acactttata cattgagttg atcgacgcta aaaacctttt cctcatcgcc
      481 agtaatcatc tccctgttga tgaactacat aatacatcac aatttattat agataatatc
      541 cttcaaacgg tacataaacc agaacgatct gtaagattag ctaagcagga ccaaggatat
      601 aagaatcatt atttatcaga tgaaatgtta gccggaaaga aagaacttta cgacttcacc
      661 cctgaaagca tttacagggc catgactata
//
LOCUS       DAZFVO020000460          686 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFVO020000460 DAZFVO020000000
VERSION     DAZFVO020000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.468"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>686
                     /locus_tag="ACHKNB_RS26895"
                     /old_locus_tag="pgaptmp_005379"
     rRNA            <1..>686
                     /locus_tag="ACHKNB_RS26895"
                     /old_locus_tag="pgaptmp_005379"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZFVO020000461          684 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFVO020000461 DAZFVO020000000
VERSION     DAZFVO020000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.469"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..315
                     /gene="yhhH"
                     /locus_tag="ACHKNB_RS26900"
                     /old_locus_tag="pgaptmp_005380"
     CDS             <1..315
                     /gene="yhhH"
                     /locus_tag="ACHKNB_RS26900"
                     /old_locus_tag="pgaptmp_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HGU4198680.1"
                     /translation="VFTYNTWAQCNNNIKIMRKYESEGKYTVRNLVKNKAIALELAEI
                     YVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGH
                     GK"
     gene            420..>684
                     /locus_tag="ACHKNB_RS26905"
                     /old_locus_tag="pgaptmp_005381"
     CDS             420..>684
                     /locus_tag="ACHKNB_RS26905"
                     /old_locus_tag="pgaptmp_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HGU4198681.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCE"
BASE COUNT          257 a           90 c          154 g          183 t
ORIGIN      
        1 gtttttacat acaacacatg ggcgcagtgt aataataata ttaaaataat gcgcaagtat
       61 gaaagtgaag gtaaatatac cgttagaaat ttggttaaaa ataaagctat agcattggaa
      121 ttagctgaga tatatgttaa gaatcgttat ggacaggatg ccgcagaaga agaaaaacca
      181 tacgaaatca ctgagttaac aacaagttgg gttgttgaag gtaccattca ctcagaccaa
      241 attgctggtg gggtttttat tatagaaata ggcaaaaatg atgggagaat tctgaatttt
      301 ggccacggaa aataagtatg taaggataat caaacatcgt gtgcgctgat ggcagagtag
      361 gtggaggact ccagacagtc aaacgataga aaaagatagc ctttatggag gttcctgcaa
      421 tgtcaaatac ataccagaaa agaaaggcaa gtaaagagta tggtttatat aatcaatgta
      481 agaaactaaa tgatgatgaa ttatttcgct tacttgatga tcacaattcc ttgaaaagga
      541 tttcatctgc cagagtatta cagttaagag gtgggcaaga cgctgttaga ttggcaattg
      601 agttctgctc tgataaaaat tatatccgta gagatatcgg agcatttata ctcgggcaaa
      661 taaaaatttg caaaaaatgc gaag
//
LOCUS       DAZFVO020000462          668 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFVO020000462 DAZFVO020000000
VERSION     DAZFVO020000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.470"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>668
                     /locus_tag="ACHKNB_RS26910"
                     /old_locus_tag="pgaptmp_005382"
     CDS             <1..>668
                     /locus_tag="ACHKNB_RS26910"
                     /old_locus_tag="pgaptmp_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGU4198682.1"
                     /translation="GQGGIPALRQVPFNIRVHAQVSGGAAGWVGEGKAKPLTKFDFES
                     ITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVADVS
                     PASITHDVKGTASSGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKNALG
                     QKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQ
                     SEPTG"
BASE COUNT          128 a          173 c          223 g          144 t
ORIGIN      
        1 ggtcagggcg ggatccctgc acttcgtcag gtgccgttca atatccgtgt gcacgcccag
       61 gtgtccggcg gtgctgccgg ctgggtgggt gagggtaagg caaaacccct gacgaagttt
      121 gattttgaat ccatcacctt cagtcatgcg aaggtgtcgg ccattgcggt actgacggaa
      181 gaattgatcc gtttttccag tccggctgct gatgcactgg tccgtaatgc gctggcggaa
      241 gcggtggtgg cgcgtctgga tacagacttt gtggacccga aaaaagcggc ggtggcagat
      301 gtctccccgg cgtccatcac ccatgatgtg aagggcacgg catcaagcgg taacccggat
      361 gcggatgcag aggctgcgtt tggacagttt gtggcagcaa acctgcagcc caccggtgcg
      421 gtctggctga tgtccagcac aaatgccctg gcactgtcca tgcgtaaaaa tgcgctgggt
      481 cagaaagaat acccggacat gaccctgctg ggtggctcct tccaggggct gccggtgatt
      541 gtctctcagt acgtgggtga ccagctggtg ctggtgaatg ccccggatat ttatctggcg
      601 gatgacggcg gcgtggcagt ggatatgtcc cgcgaggcat cactggaaat gcagtctgag
      661 ccgaccgg
//
LOCUS       DAZFVO020000463          654 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFVO020000463 DAZFVO020000000
VERSION     DAZFVO020000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.471"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..504)
                     /locus_tag="ACHKNB_RS26915"
                     /old_locus_tag="pgaptmp_005383"
     CDS             complement(<1..504)
                     /locus_tag="ACHKNB_RS26915"
                     /old_locus_tag="pgaptmp_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HGU4198683.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTCVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSS"
BASE COUNT          197 a          158 c          126 g          173 t
ORIGIN      
        1 cgaagaacct aatcttaaat agcctccatc gacgtgaccc ttctgtttaa tattcagcgt
       61 ccccgttccg gattgaccca catttaaagg ccttgcatta tttccgctat catacaatcg
      121 ccaggtgcca cccaaaacat taacggtgcc ctctgagtcc tcattagcgc caatgacgca
      181 ggtagtaata gtggttaagg aggcattacc gccagccaaa atattaagca ctccagtgcc
      241 ataccagcca accaggtagg catcataagc aacatcagta tcaatattgg taatctgatc
      301 attatcaact tccagacttg caccagacgc tgaaccgctt agtgccagca aaacacccag
      361 cgtcaatcta ctgaatttcg ttgtcagtcc agaggattta cgcaaattaa ccgtcgatgt
      421 tttacctgcc ctgcgagtta attccgagca ggcctgaaat acctgtagag tgcaattcca
      481 tatcacgcga tagattctat tcatataaac gctccatata caaacaatac agccacgaaa
      541 gagatgagtc gtcagacatt aaaaatgaaa cttattaaat tgtcagaggt ctgtattgag
      601 tgttagttgg aggttggaag gtgtcaattt ggctaatttg ctaatgcttt cgaa
//
LOCUS       DAZFVO020000464          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFVO020000464 DAZFVO020000000
VERSION     DAZFVO020000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.472"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            12..474
                     /locus_tag="ACHKNB_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /pseudo
     CDS             12..474
                     /locus_tag="ACHKNB_RS26920"
                     /old_locus_tag="pgaptmp_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(513..>644)
                     /locus_tag="ACHKNB_RS26925"
                     /old_locus_tag="pgaptmp_005385"
     CDS             complement(513..>644)
                     /locus_tag="ACHKNB_RS26925"
                     /old_locus_tag="pgaptmp_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198684.1"
                     /translation="DAYVDEVLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
BASE COUNT          183 a          146 c          141 g          174 t
ORIGIN      
        1 agtcatcaac aatgattcag ggcggtgatt atttacttaa agaaaacgaa gagttaggtg
       61 gtgaaaacga tcataaaatg atcattttat tgacttttta tccagtgaag gtaggatcgc
      121 gcgtatatgt agtatgtttc aaatcaacag gatactacat aaaagggaaa cccagcatag
      181 ctggaactat gctgggtcaa tctgtaaaaa cttgcatcaa tgctttactt cgagctcgtg
      241 gcgtatccta tgatattccc cggtctcctg taaagcagag atgcataaaa aggagatctt
      301 tatcatggat aaagagcctg tttgccctgc ctgtaagact cccttacagc tcatctatcg
      361 ccgttctcga tctcaaaatg gcaagacata caaaccaaaa atgcttcctg tttcaggatg
      421 ctgatatgtc ctgcctgttc tccccggagg gaaaatacac aaaacgtgca ttaacaaatt
      481 cacccggcag ggaaacctgc cggtcggtat tgttacatca tagtaccgtc agttccgggc
      541 tggaccggct ctggttgctc tacgggttga ggctcatttt ccgtgctgtt ttcagccgga
      601 gaacgaccaa ccattcgggc cagcacttca tcaacatatg cgtc
//
LOCUS       DAZFVO020000465          644 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFVO020000465 DAZFVO020000000
VERSION     DAZFVO020000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.473"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..644
                     /locus_tag="ACHKNB_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /pseudo
     CDS             1..644
                     /locus_tag="ACHKNB_RS26930"
                     /old_locus_tag="pgaptmp_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          205 a          112 c          155 g          172 t
ORIGIN      
        1 gtgccgttat ttctggtgca gaaggctggg aagatataga ggattttggg gaaacacatc
       61 ccgatgtttt gaagtgatat ggtgattttg aaaatggtat tcctgttcac gatatcattg
      121 ccagagttgt atcctgtatc agtcctgcaa aatttcacga gtgctttatt aactggatgc
      181 gtgactgcca ttcttcagat gataaagacg tcattgcaat tgatggaaaa acgctccggc
      241 actcttatga caagagtcgt cgcaggggag cgattcatgt cattagtgcg ttctcaacaa
      301 tgcatagtct ggtcctcgga cagatcaaga cggatgagaa atccaatgag attacagcca
      361 ttcctgaact tcttaacatg atggatatta aaggaaaaat catcacaact gatgcgatgg
      421 gctgccagaa agatattgca gaaaagatac aaaaacaggg aggtgattat ttattcgcgg
      481 taaaaggaaa ccaggggcgg ctaaataaag cctttgagga aaaatttccg ctgaaagaat
      541 taaataatcc agagcatgac agttacgcaa tgagtgaaaa gagtcacggc agagaagaaa
      601 tccgtcttca tattgtttgc gatgtccctg atgaacttat tgat
//
LOCUS       DAZFVO020000466          638 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFVO020000466 DAZFVO020000000
VERSION     DAZFVO020000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.474"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            567..>638
                     /gene="hokD"
                     /locus_tag="ACHKNB_RS26935"
                     /old_locus_tag="pgaptmp_005387"
     CDS             567..>638
                     /gene="hokD"
                     /locus_tag="ACHKNB_RS26935"
                     /old_locus_tag="pgaptmp_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HGU4198685.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALV"
BASE COUNT          173 a          133 c          153 g          179 t
ORIGIN      
        1 ctacttatat tgatattagc aatgtaattg ctaaagtatt cctagcccac tattcatcta
       61 aatatggttg tcgaaaggcc cgcttctcgc tcaaagcgga ctagaaggtt agcttgtgtc
      121 ggactttgcg tattaaatga ctagtggttg agccccattt ccacagaaaa aatcagagaa
      181 actataccca atagttgtat tgaatcactg acgagacagc ctcatattca tcaggactgg
      241 tgtacgtcca atacaggagg ttgtggtgct ggttctcaaa tgtgcgctgg ctattacggc
      301 tgtaatggcg atttattgtc ttgctattgt tcttatggat cgcctttctg actgatttca
      361 cattggcgag gtaacggtag ttaagtagaa tggctgcggg tgcttgaggc tatctgcctc
      421 gggcatgaac accaacggca gatagagaaa agccccagtt aacattacgc gtcctgcaag
      481 acgcttaaca ttaatctgag gccaatttca tgctagacat atgtaggtta gcctcttacg
      541 tgccgaaagg caaggagaag caggctatga agcagcaaaa ggcgatgtta atcgccctga
      601 tcgtcatctg tttaaccgtc atagtgacgg cactggta
//
LOCUS       DAZFVO020000467          634 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFVO020000467 DAZFVO020000000
VERSION     DAZFVO020000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.475"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>634
                     /locus_tag="ACHKNB_RS26940"
                     /old_locus_tag="pgaptmp_005388"
     CDS             <1..>634
                     /locus_tag="ACHKNB_RS26940"
                     /old_locus_tag="pgaptmp_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGU4198686.1"
                     /translation="TKVVDSGWYIQGEEVRNFEHEFASFIGSKYCVGVANGLDALSIT
                     LKAWILLGKIKEGDEVIVPANTFIATVLAITQNHLKPIFVEPDEETFNINVDMIRNVI
                     TEKTKVIIPVHLYGQVAEMNKIRELADLHDLLILEDSAQAHGAMYNNLKAGNLGDAGA
                     FSFYPGKNLGALGDAGAVVTDNQELAETIRAIGNYGAHVKYKHDVQGVNSR"
BASE COUNT          209 a           92 c          148 g          185 t
ORIGIN      
        1 aactaaagtt gttgattcgg ggtggtatat tcaaggtgaa gaagtcagaa atttcgaaca
       61 tgagtttgca tcatttattg gttcgaagta ttgtgtcggt gttgccaatg gcttagatgc
      121 attgagcatt accttaaaag catggatatt attaggcaaa attaaagaag gggatgaggt
      181 aatagttcca gcaaatacat ttattgcaac tgtattagct atcacccaaa atcatcttaa
      241 accaatattt gtagaaccag atgaagaaac attcaacatt aatgttgata tgattagaaa
      301 tgtcatcacg gagaagacta aagttattat acctgttcat ctttatgggc aggtggcaga
      361 aatgaataaa attcgtgaat tagctgacct tcatgatctg ttgattttgg aggactctgc
      421 gcaagcacat ggagccatgt ataataacct taaggcagga aaccttggtg atgcaggcgc
      481 ttttagtttc tacccaggta aaaacttggg ggcattaggg gatgctggtg ctgtagtgac
      541 tgacaatcaa gagctggcag aaactataag agcaattggt aattatggtg cacatgtaaa
      601 atataaacat gatgtacaag gggttaatag ccgt
//
LOCUS       DAZFVO020000468          633 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFVO020000468 DAZFVO020000000
VERSION     DAZFVO020000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.476"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>633
                     /locus_tag="ACHKNB_RS26945"
                     /old_locus_tag="pgaptmp_005389"
     CDS             <1..>633
                     /locus_tag="ACHKNB_RS26945"
                     /old_locus_tag="pgaptmp_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HGU4198687.1"
                     /translation="AGFNKVITDVGPGKLNLGGCVIEVMGTYNAAIVRVTIGEYGATG
                     FINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTGSNDET
                     KAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDTGPAGPAGPQGPKGDTGA
                     AGPAGAKGPKGDKGDPGEAGPAGLAGPKGERGEPGPMGPPGKDGATSLVSG"
BASE COUNT          172 a          139 c          226 g           96 t
ORIGIN      
        1 tgcgggattc aataaagtca tcacggatgt cgggccggga aaactcaatc tgggtggctg
       61 tgtcattgag gtgatgggga cgtataacgc agccatagtc cgggtcacca ttggcgaata
      121 cggtgcaacg gggtttatta atggcacggt ctgtacctgt acggtttacg gcgacacaca
      181 gtatttccac tggcgggtgg attacagcac aaaaaacaaa ccggatacgg tcagccagcg
      241 ggatgccagc acgacgcaga aaggtgtggt gcagttaagc agtgatactg gcagtaatga
      301 cgaaacaaag gcagccacgc cgaaggccgt gaaggcggca atggatgtgg caaatgaagc
      361 gaaaacaaag gcagaagagg ctgcagcagg aggtggtgtt cccggtccga aaggagagaa
      421 aggggacacg gggccagcag gtcctgcagg accgcaggga cctaaagggg atacgggagc
      481 tgcaggcccg gcaggcgcaa aggggccaaa aggtgacaaa ggcgatccgg gagaagcagg
      541 tccagcaggt ctggcaggtc ccaaaggtga gcgcggtgaa ccgggaccga tggggcctcc
      601 ggggaaagat ggcgcaactt cgctggttag tgg
//
LOCUS       DAZFVO020000469          633 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFVO020000469 DAZFVO020000000
VERSION     DAZFVO020000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.477"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..386)
                     /locus_tag="ACHKNB_RS26950"
                     /old_locus_tag="pgaptmp_005390"
     CDS             complement(<1..386)
                     /locus_tag="ACHKNB_RS26950"
                     /old_locus_tag="pgaptmp_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4198688.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERA"
BASE COUNT          192 a          115 c          133 g          193 t
ORIGIN      
        1 ttcgcacgtt cagcacaatc agttgtcatg cactcggaat aaatcatacc gtgaatagtg
       61 ctgtttttga ttgtcagact attggcgttg gtattggtag aaataccgtc gtccagatag
      121 taggtagaaa tcacgccgtt aacagtagcg ttattaatga ccgggtagat atcgccgttg
      181 taataactat cagcggcata gttgttccag cctacataac catcatagta aacgtcatca
      241 gcataggtgg cgtcgttgta gtgatggaaa gtattgtaag tttgaccaga aatatcgttg
      301 gtcgctgcat tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact
      361 acaagtttct ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat
      421 ccatcaatta tcaatgcata gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt
      481 acagacgaat ggttcaatta ttctttcccc taagtccgaa tgtggacata tataatttat
      541 ggagaaaaac acgcatcaaa ttgataaaag gacgatattt caacatgttg cagataatgt
      601 tatttttgat acatacgcgt tataaatatc aaa
//
LOCUS       DAZFVO020000470          630 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFVO020000470 DAZFVO020000000
VERSION     DAZFVO020000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.478"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            126..>630
                     /locus_tag="ACHKNB_RS26955"
                     /old_locus_tag="pgaptmp_005391"
     CDS             126..>630
                     /locus_tag="ACHKNB_RS26955"
                     /old_locus_tag="pgaptmp_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198689.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGN"
BASE COUNT          184 a          118 c          145 g          183 t
ORIGIN      
        1 attaaagatt tgtcacttat tggatttagt atgcttgatt taaatggcgg tcaattattg
       61 ttattattaa gtctaatgtc atttaaaggc attatcatat ttacgaagag atcaggatga
      121 tgacgatgaa aaaaagtgta ttgacggcgt ttataactgt ggtatgtgca acgtccagcg
      181 ttatggcagc tgatgataat gctatcacgg atggcaaagt aacatttaat ggtaaagtta
      241 ttgctccagc ttgtaccctg gtagctgcga cgaaagattc agtagtcaca ttgccaaatg
      301 tcagcgccac caagttgcaa actaatggag cagtctctgg cgttaaaact gatgtaccaa
      361 ttgccttaga aggctgtgat gttactgtaa ctaaaaatgc gacgtttacc tttagtggta
      421 cggctgacgg cgtgcaacct acagcatttg ccaaccaggc taccaccgat gcagcgacga
      481 atgttgctct acaaatgtat ctgccagatg gttctacatc ggtaactccc ggtaccgaaa
      541 cgtcgaatat tcagttagcg gattctgctg agcaaacagt gacctttaaa gttgattata
      601 tcgctacggg gaaagcgact tctggtaatg
//
LOCUS       DAZFVO020000471          627 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFVO020000471 DAZFVO020000000
VERSION     DAZFVO020000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.479"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..245
                     /locus_tag="ACHKNB_RS26960"
                     /old_locus_tag="pgaptmp_005392"
     CDS             <1..245
                     /locus_tag="ACHKNB_RS26960"
                     /old_locus_tag="pgaptmp_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HGU4198690.1"
                     /translation="WKNNISDALVFDYSACYDAEEDSRKPVYANWGSGSNGFAVCDGK
                     AGYGAAGFRAYRGKPGYSGSMVWVKIRCCKLKVNTP"
     gene            complement(251..>627)
                     /locus_tag="ACHKNB_RS26965"
                     /old_locus_tag="pgaptmp_005393"
     CDS             complement(251..>627)
                     /locus_tag="ACHKNB_RS26965"
                     /old_locus_tag="pgaptmp_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198691.1"
                     /translation="WAGFESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAY
                     PGCKLPDLRGVFIRGWDNGRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYW
                     AQGSTTEMRPKNIAFNYIVKAS"
BASE COUNT          157 a          152 c          173 g          145 t
ORIGIN      
        1 tctggaagaa taatatcagc gatgcactgg tttttgatta ttcggcctgt tatgacgcag
       61 aagaagactc cagaaaaccg gtttatgcga actggggtag tggctcaaac ggctttgccg
      121 tctgtgacgg caaagcaggc tatggtgcgg caggattcag agcgtacaga ggtaaaccgg
      181 ggtattcggg aagcatggtc tgggtcaaaa tacgttgctg taagttaaaa gttaatacgc
      241 cttaaatgca tcagcttgct ttgacgatgt agttaaaggc aatgtttttg gggcgcattt
      301 ccgttgtgct tccctgggcc cagtaaggca taccgctgtg atgccctttc agccatcccc
      361 cacgctcatg atagacaccg gattgatcgg cctgataact caggagatgt ctgttccgat
      421 ctccatccag cccacgtccg ttgtcccagc cacggataaa aacaccgcgc aaatccggca
      481 gcttacaccc gggataagca cgggcaagct gaggataccg ggaacatgaa aatgactgcc
      541 cgttcatcac caggtagcct tgtggagggg tggctgatgg ccacggtata gggcttccga
      601 cagggtagga ttcaaaacct gcccaca
//
LOCUS       DAZFVO020000472          626 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFVO020000472 DAZFVO020000000
VERSION     DAZFVO020000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.480"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            30..596
                     /locus_tag="ACHKNB_RS26970"
                     /old_locus_tag="pgaptmp_005394"
     CDS             30..596
                     /locus_tag="ACHKNB_RS26970"
                     /old_locus_tag="pgaptmp_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198692.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
BASE COUNT          171 a          153 c          144 g          158 t
ORIGIN      
        1 ttttttcttt tctgttaaag gactccgtta tgtttaaagg acaaaaaaca ttggccgcac
       61 tggccgtatc tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa
      121 ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata
      181 aagaggttga actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg
      241 ccgttgctgt cgacctgcgc ttagtcaact gtgatctgga aaactccagc aacggttccg
      301 gtggcaagat ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag
      361 atccgattct caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga
      421 ataaagatca aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga
      481 cctccagcga acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac
      541 ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat
      601 tactttggcg gcgctatcgc cgcctt
//
LOCUS       DAZFVO020000473          614 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFVO020000473 DAZFVO020000000
VERSION     DAZFVO020000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.483"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..340)
                     /locus_tag="ACHKNB_RS26975"
                     /old_locus_tag="pgaptmp_005395"
     CDS             complement(<1..340)
                     /locus_tag="ACHKNB_RS26975"
                     /old_locus_tag="pgaptmp_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGU4198693.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVREAASGMPD
                     PTLPEVSGGSG"
     gene            382..582
                     /locus_tag="ACHKNB_RS26980"
                     /old_locus_tag="pgaptmp_005396"
     CDS             382..582
                     /locus_tag="ACHKNB_RS26980"
                     /old_locus_tag="pgaptmp_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HGU4198694.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
BASE COUNT          167 a          176 c          144 g          127 t
ORIGIN      
        1 tgccactacc tccggacacc tccggcagtg tcgggtccgg cataccactc gccgcttcac
       61 gaacagactt ttgtcgatgg cattcggtac agagcgtcca gagattcgtc tcctcattac
      121 caccaccgaa ctgaagtgca attcggtgat cgagttcact gtcacagagg tcaaccacac
      181 gaccacagag acagcactgc ccggcgtccc tgagccagat atgacgcttg agggaaacac
      241 gtgcactgcc actgacacga cgctgttcac ccttcagaat attcacccgt cgggtattca
      301 gtgttttgat tctgctctgg agtgtacgaa gctgagccat gtaaaatccc cgtcatatgg
      361 caatcagtaa aggaaataaa tatgtcatcg aaaaaccgga cccgcagaac cacaacccgc
      421 aatatccgtt tccccaatca catgattgaa cagatcaaca tcgcccttga gcataaaggg
      481 tccggtaact tttcagcgtg ggttattgaa gcctgcagga gaaggctggc aacagatgca
      541 acgcatctgc gcccggccag catgacaaat aacgagaaat gaacgttcgg ttacaggagc
      601 aggtacccac tgtc
//
LOCUS       DAZFVO020000474          607 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFVO020000474 DAZFVO020000000
VERSION     DAZFVO020000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.484"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..>607
                     /locus_tag="ACHKNB_RS26985"
                     /old_locus_tag="pgaptmp_005397"
     CDS             38..>607
                     /locus_tag="ACHKNB_RS26985"
                     /old_locus_tag="pgaptmp_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198695.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSIFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQK"
BASE COUNT          245 a          123 c           88 g          151 t
ORIGIN      
        1 aaccttagca taaaacagat gatgaataag gaatgttatg tcaattgact ttaccccagg
       61 tataataaat acatatcacg gcgatattta taactgcaca acaaataccg ataatgttaa
      121 aacgccagac acaccgaagt ggccttgtga taattgggaa gaacaacaac ccattaattc
      181 catattctct ggagaaggat acaaccctga acaatttgat ttagcgcaac aacaactcca
      241 acaaattaat gcatgccaca cgaacaccac atatacaaat gcagactact caaaagttgt
      301 ggttcaactt gtaagtctta ttaataacat tgaaacgata agttcgacac cacttacgca
      361 acagacacaa tcaatactta atcagattaa taacattaga tatgaaaaaa acaaaagtgc
      421 tgaatgtcga atcattgttg tcgctaatcc taaacccgac aaggcaatca taactaaaat
      481 tagcgtagaa gagggaatac ccataacatt cagtgtccaa accatgtttt cagatactaa
      541 ttttattgct gaacaacgag ctgacttacc aactaatata aaagatattc aaagcctgta
      601 tcaaaaa
//
LOCUS       DAZFVO020000475          596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFVO020000475 DAZFVO020000000
VERSION     DAZFVO020000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.485"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>596
                     /locus_tag="ACHKNB_RS26990"
                     /old_locus_tag="pgaptmp_005398"
     CDS             <1..>596
                     /locus_tag="ACHKNB_RS26990"
                     /old_locus_tag="pgaptmp_005398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGU4198696.1"
                     /translation="NLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFS
                     GNIRIGRFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITK
                     PENPIIGNDVWIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAK
                     IIRFRFDSNVIDELLRIKWWNYNYSDLPDNNKCDDINY"
BASE COUNT          191 a           85 c          122 g          198 t
ORIGIN      
        1 aatttatttc ttttctcaaa aggtgacaag ataacaattg aagataatgt tattgctgaa
       61 gaatattcaa caatgcctgt gaaaaatttc tccagtgtcg gcgctttttc atttccgacc
      121 tgccattttt cagggaatat acgtataggt cgattttgta gtattgcatc aaatgtaaaa
      181 ataatgggtg gaaatcatcc tttgaatcgt tttactactc acatgatgac ttataacggt
      241 gaatttgata agtttgctat gagtgaattt gaacgtagct ggacattgaa accattcatt
      301 accaaacctg aaaatccgat tataggaaat gatgtctgga taggaaatga tgtctggata
      361 ggaaatgatg ttgtattgaa aggtggcatc gcaataggtg atggtgctgt tattgctgcc
      421 aattccgttg ttaccaaaga tgtgccccca tatgccatag ttgcaggagt tcctgcaaaa
      481 ataatcagat ttaggtttga ttcaaatgtt attgatgaac tgttgagaat aaaatggtgg
      541 aattataact actcagatct tccggataat aacaagtgtg atgatataaa ttattt
//
LOCUS       DAZFVO020000476          596 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFVO020000476 DAZFVO020000000
VERSION     DAZFVO020000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.486"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..488
                     /locus_tag="ACHKNB_RS26995"
                     /old_locus_tag="pgaptmp_005399"
     CDS             <1..488
                     /locus_tag="ACHKNB_RS26995"
                     /old_locus_tag="pgaptmp_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4198697.1"
                     /translation="YPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEY
                     GGLICKKQGEYLPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTK
                     ENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGS
                     N"
     gene            490..>596
                     /locus_tag="ACHKNB_RS27000"
                     /old_locus_tag="pgaptmp_005400"
     CDS             490..>596
                     /locus_tag="ACHKNB_RS27000"
                     /old_locus_tag="pgaptmp_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HGU4198698.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAA"
BASE COUNT          228 a           74 c          121 g          173 t
ORIGIN      
        1 catatccgct gagcccggtg aatagcatgg atccattagg attatatgaa tttaaatcaa
       61 aaaatataga tgatattgga atatttgcat tggcaatgtg taatggagaa tcaattaacg
      121 agaataaaga atatggtgga ctaatatgta agaagcaagg tgaatattta cccatgaatc
      181 cgataagttc aaatgataat gatagtgtag acttgcgaaa tataaaatgc cctgaaggtt
      241 cagagagagt aggcgattat cacactcacg gtttttactc tgacgataaa ggaaataaag
      301 taacaaaaga aaatgatgtt tatgatagtc taaatttttc aagcaaagat ttaacgaatt
      361 cttatatgaa tggaatggga aaaaaagaat acagtagtta cttgggaaca ccaaataaca
      421 cctatctaaa atataatccc aaagctaaag ggaatggagt tacaattatc aggcaaggga
      481 gtaattaaaa tgaaggcaat acaatgttgt tatttccttg ctttagtctc cagtcattta
      541 atggctggag ttatgggtga tgataatata tttttaagta attcggattc agctgc
//
LOCUS       DAZFVO020000477          595 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFVO020000477 DAZFVO020000000
VERSION     DAZFVO020000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.487"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..150
                     /locus_tag="ACHKNB_RS27005"
                     /old_locus_tag="pgaptmp_005401"
     CDS             <1..150
                     /locus_tag="ACHKNB_RS27005"
                     /old_locus_tag="pgaptmp_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198699.1"
                     /translation="NGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGG
                     NYGLY"
     gene            137..>595
                     /locus_tag="ACHKNB_RS27010"
                     /old_locus_tag="pgaptmp_005402"
     CDS             137..>595
                     /locus_tag="ACHKNB_RS27010"
                     /old_locus_tag="pgaptmp_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HGU4198700.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTALVIGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINAIPASDDTAPAVAEDTAAVLEQKSGSAMNQLFNTLSD"
BASE COUNT          135 a          154 c          189 g          117 t
ORIGIN      
        1 aatggcggac aggccaccgc gaaaaccgcc gcacgggtgg agcttgtggc ccctgaaggc
       61 tggcgggcac gcagtattga tgtggatgat accgcacagc atggcctgga tgtgaacgtc
      121 tggaacggag ggaactatgg cctctactga cacgccgggg cgtggctcct ttcgtggtgt
      181 accgtttctg gtttatcagg aacaacggga acggggcgga cgtaacattg tacgccgtga
      241 atatcctctg cgggaaagcg gcggcgcgga tgatttaggg ccaaaactcc cggagtttac
      301 attcacggct cttgtgatcg gcgatgatct tcagacgcag cgaagccgcc tgcgggatgc
      361 gctgcgggca cctggtgccg gtgaactgat gcacccggat tacggcacct taaatgtgct
      421 gataaacagc tttgaaagcc gttataacgc cagcgaacag ggaacggttg agttcacgat
      481 caacgcgatc cccgccagtg acgataccgc accggcggtg gctgaagaca cggctgccgt
      541 tctggagcag aaaagcggtt cagcaatgaa tcagttgttt aacacgcttt cagac
//
LOCUS       DAZFVO020000478          590 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFVO020000478 DAZFVO020000000
VERSION     DAZFVO020000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.488"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>590
                     /locus_tag="ACHKNB_RS27015"
                     /old_locus_tag="pgaptmp_005403"
     CDS             <1..>590
                     /locus_tag="ACHKNB_RS27015"
                     /old_locus_tag="pgaptmp_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4198701.1"
                     /translation="AYGYAIVNKTFEGTVAISRDDFEDDNLGIYSPLFQEMGRAAAVQ
                     PDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQA
                     DYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGASARRNVGY
                     GFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLG"
BASE COUNT          120 a          159 c          175 g          136 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaact tttgaaggca ctgtcgccat tagccgcgat
       61 gacttcgaag acgacaacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gcggcggtac aaccggatga gctggtcttt gctgccctgc gtgacgggat cagtgcggcc
      181 tgctatgacg gtcagaactt ctttgatacc gaacatccgg tttaccccaa agtggatggc
      241 tccggggatg cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggcgca
      301 caggctgatt acagcggtcc ggcctggtat ctgcttgact gctcccgcgc ggtaaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt
      421 cgcgcattca ccgataacga atttgtgttt ggtgcatccg cccgtcgcaa tgtgggctac
      481 ggcttctggc agatggccta catgatgcag tcaccgctga cgctggatgc actgtggcac
      541 ggctggtcag ccatgcgcga atttaccgct gacggtggcc gcaaactcgg
//
LOCUS       DAZFVO020000479          587 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFVO020000479 DAZFVO020000000
VERSION     DAZFVO020000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.489"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>587
                     /locus_tag="ACHKNB_RS27020"
                     /old_locus_tag="pgaptmp_005404"
     CDS             <1..>587
                     /locus_tag="ACHKNB_RS27020"
                     /old_locus_tag="pgaptmp_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4198702.1"
                     /translation="VLADLQRTFEGKIAAQAQTFEEKLASLSVVLQKCVTGDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVGKIPVPANGKSITPDDVRPMIEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVKEAV
                     SHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGK"
BASE COUNT          142 a          128 c          189 g          128 t
ORIGIN      
        1 gtgctggcgg atttacagcg cacatttgag gggaaaatag ccgcgcaggc acaaacgttt
       61 gaagaaaaac tggcttccct gtctgtggta ttacagaagt gcgtgacggg cgatgatgtg
      121 cgtccgatgc ttgagcagat ggtgaaggag gcggtaagcc atatccctgt tccgcgcgac
      181 ggtcgtgact atgatcccga tgttctgcag aaggcggtga atgatgctgt cgggaaaata
      241 ccggtaccgg cgaacggtaa aagtattact cccgatgatg tgcgtccgat gattgagcag
      301 atggtgaagg aggctgtaag ccatattcct gttccgcgcg acggtcgtga ctatgatccc
      361 gatgttctgc agaaggcggt gaatgatgcg gtcgcaaata ttccgcagcc ggcggacggt
      421 aaaagtctca ccccggatga tgtgcgtccg atgcttgaac agatggtgaa agaggcagtg
      481 agccatattc ctgttccgcg tgatggtcgt gactacgatc cggaagtact gcagaaggcg
      541 gtgaatgatg cagtcgcaaa tattccgcag ccggcggacg gtaaaag
//
LOCUS       DAZFVO020000480          586 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFVO020000480 DAZFVO020000000
VERSION     DAZFVO020000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.490"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            38..>586
                     /locus_tag="ACHKNB_RS27025"
                     /old_locus_tag="pgaptmp_005405"
     CDS             38..>586
                     /locus_tag="ACHKNB_RS27025"
                     /old_locus_tag="pgaptmp_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198703.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLN"
BASE COUNT          160 a          124 c          171 g          131 t
ORIGIN      
        1 ggcggcattg caggcgcttt gttttaagga gtggtgaatg agcggatttg cacaggggtt
       61 acttgccgga ttcagcactg ttgaccaggc aatgacccgt cgtaaggagc ttggtttgcg
      121 agaagcacag cttgctcagc aacagaaaaa taacgagcgc gattttgaat ttgcgcagtc
      181 tcagtttgaa cataataaag acgttgatca gcggaacttt gattacagag ccaaagttga
      241 tgaccgcaat tacacactgc aggaaaggga gtttaacgcc aaccagaatt accggaatgc
      301 gtcgctgggg atggaacagc aacgactcca gttgcagaaa tacaaccagc gacggcttga
      361 gtataacgat atgctggcgc gcgatcagcc tgtgatggcg gcgctgggaa aagcgattga
      421 tgcgggtgac catgatgcgg cttctcatct gttcggtaaa ttatcggacg ccaatccgct
      481 taagatgatg tcaacggtag gctatgctgc gaaagcgggt caggccgtga acaacctgca
      541 gaaaatcttt gatgataagc cggacagggc gattgcttcg ctgaat
//
LOCUS       DAZFVO020000481          584 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFVO020000481 DAZFVO020000000
VERSION     DAZFVO020000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.491"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..327
                     /locus_tag="ACHKNB_RS27030"
                     /old_locus_tag="pgaptmp_005406"
     CDS             <1..327
                     /locus_tag="ACHKNB_RS27030"
                     /old_locus_tag="pgaptmp_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4043 family protein"
                     /protein_id="HGU4198704.1"
                     /translation="RKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDRAMLLGA
                     QALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQDHGVIAV
                     DTAVKL"
     gene            383..>584
                     /locus_tag="ACHKNB_RS27035"
                     /old_locus_tag="pgaptmp_005407"
     CDS             383..>584
                     /locus_tag="ACHKNB_RS27035"
                     /old_locus_tag="pgaptmp_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198705.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATV"
BASE COUNT          142 a          137 c          164 g          141 t
ORIGIN      
        1 cgtaagtatg cgggtatgcc gatccgtttc tatcaggggt caaaggttct ggtatcagag
       61 aataacctga cggcaaccac gaaagaggtc gctgctgcaa ccaatattga ccgcgccatg
      121 ttactggggg ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg tcacttcaac
      181 atggttgaga agaaaacgga tatggataac cgtactgaga tagcaatcag ctggatcaac
      241 ggtctgaaaa aaatccgttt ccccgagaag agcggcaaga tgcaggatca cggcgtgatt
      301 gccgttgata cagcagtgaa gctctgattt tttcctttcc ccatgccggg ttttcgcccg
      361 gctttttcag gagtcattaa ttatggcaaa gactatcctt gccccgtcac tgagtgaacg
      421 ggtctatacg ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta cggtgaatgc
      481 tgcggaagcg gacagtgtta ttcatcttct ctcactgcca gtgggcatcc gtatcaactc
      541 actccagctg gtttcaacgg gtggtctggg tactgcaacc gtca
//
LOCUS       DAZFVO020000482          582 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFVO020000482 DAZFVO020000000
VERSION     DAZFVO020000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.492"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>582)
                     /locus_tag="ACHKNB_RS27040"
                     /old_locus_tag="pgaptmp_005408"
     CDS             complement(<1..>582)
                     /locus_tag="ACHKNB_RS27040"
                     /old_locus_tag="pgaptmp_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HGU4198706.1"
                     /translation="TPPGTSQGRGQRFINFLTGVNPAAVQPVLVTNWPTGGLSGTGAT
                     DTSSGGRQGRGRRKRGPGRGRPVTPPLPPSPVPPSPSSGGTGFWGRMMGRAGGLLSSV
                     GSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVLLDGSASATDKGEAVGSLAGS
                     IAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLT"
BASE COUNT          112 a          212 c          167 g           91 t
ORIGIN      
        1 gtcagccatc cccccagata gttaccgaga tagctgccca ccgtagaacc aatggcaatc
       61 cccacaggtc cggctgccgc ccccagcgca ccaccggcaa tactgcctgc aagagagccg
      121 accgcttctc ccttgtctgt ggcactggcg ctgccatcca gcagaaccgg ggctgccatc
      181 gcgccagccc acagggcacc tccacccagc cggccggcca gtccaccggc actgcggaaa
      241 aagccggaga aacgccccag ccccatgcgg ctaccgacag acgaaagtaa cccaccggcg
      301 cgccccatca tacgccccca gaaccctgtc ccaccagaag agggagacgg tggcactggg
      361 gacggtggca ggggaggcgt gaccggtctt ccgcgccccg gtccgcgttt cctgcggccc
      421 cgcccctgac gtccgcctga ggatgtatcg gtcgcgccgg tgccggacag tcctcctgtg
      481 ggccagtttg ttacaagtac tggctgaacg gctgccggat ttaccccggt aagaaagttt
      541 ataaaacgtt gccctcgtcc ctgagatgtg cctggtggtg tt
//
LOCUS       DAZFVO020000483          575 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFVO020000483 DAZFVO020000000
VERSION     DAZFVO020000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.493"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(46..459)
                     /locus_tag="ACHKNB_RS27045"
                     /old_locus_tag="pgaptmp_005409"
     CDS             complement(46..459)
                     /locus_tag="ACHKNB_RS27045"
                     /old_locus_tag="pgaptmp_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198707.1"
                     /translation="MKKFMLLIFSVSFFLTGCATIVGDKTQTVQVNSNPSGADFIIKD
                     ESGKTISSGKTPQNVTLQKSDGSYFGKKSYQITFSKENYQPVTLPIKASANGWYIGGN
                     FVFGGIVGWLLVDPFNGGMYTLSPESLNPGLVPKT"
BASE COUNT          174 a          135 c          107 g          159 t
ORIGIN      
        1 aaaaaaacct gccgcagcag gttttattca tcagcagatt attgattaag ttttgggtac
       61 cagcccgggg ttcagacttt ccggactcag tgtatacata ccgccattga atgggtccac
      121 aagcaaccag ccaacaatac caccaaagac aaagttacca ccaatgtacc atccattagc
      181 cgatgccttg atcggcagcg ttacgggctg ataattctct ttactgaatg tgatctgata
      241 actctttttc ccgaagtaac tgccgtcaga tttttgaagt gttacattct gcggtgtttt
      301 gcccgaagaa attgttttcc ctgactcatc tttaataata aaatccgcac cagacggatt
      361 actgtttacc tgtacagtct gcgttttatc tccgacaatg gttgcacacc ccgtcaggaa
      421 aaagctgaca gagaaaatca acagcatgaa tttcttcata aaatggctcc gttcagttaa
      481 ttttccccga aaatactgta ataaagatct gcaaagttaa aattaatttt ccgaagcaaa
      541 taacagagca tcctccagag cggccaaagg atctg
//
LOCUS       DAZFVO020000484          567 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFVO020000484 DAZFVO020000000
VERSION     DAZFVO020000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.494"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>567
                     /locus_tag="ACHKNB_RS27050"
                     /old_locus_tag="pgaptmp_005410"
     CDS             <1..>567
                     /locus_tag="ACHKNB_RS27050"
                     /old_locus_tag="pgaptmp_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HGU4198708.1"
                     /translation="TRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVVTRQDA
                     ETPNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     SSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTY"
BASE COUNT          129 a          145 c          187 g          106 t
ORIGIN      
        1 aaacgcgtga taccggcttc agtcatatca cacctgttgt cagggctgaa caggggacgg
       61 gcggcacggc ctggcagctg aatgttgtgc agccggaaac tgccgcagag cctgtggtga
      121 cgcggcagga tgcagagacg ccgaatcctg tggaagctgt atcaccgctc ccttcaccgg
      181 tttctgctcc gtcaccggct ccggaggcgt cagtgacgga tgtactgacc ggagagaatg
      241 cgggggaatc aggtgagtac cgtgatgaga cccggtggct gctgacagga taccggagta
      301 cggttaatag ctccgcggtc agggatgcgg ggatgctgat gtcaatgggg caccgtaact
      361 tcattaatga ggtcaacaac ctgaacaaac gtatgggtga cctgcgggac atcaacggcg
      421 aggccggtgc atgggcccgt atcatgagcg gaaccggctc tgccggcggt ggcttcagtg
      481 acaactacac acacgttcag gtgggtgcgg ataaaaaaca cgaactggac ggtctggacc
      541 tcttcaccgg ggtgaccatg acatata
//
LOCUS       DAZFVO020000485          556 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFVO020000485 DAZFVO020000000
VERSION     DAZFVO020000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.495"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..222
                     /locus_tag="ACHKNB_RS27055"
                     /old_locus_tag="pgaptmp_005411"
     CDS             <1..222
                     /locus_tag="ACHKNB_RS27055"
                     /old_locus_tag="pgaptmp_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198709.1"
                     /translation="DKGRTKDQNATAVNARRHGDFAVALCMANRASYMEGFILDESAC
                     QALPERSRAMEGGYRNDDEAYHEFDRGCW"
     gene            222..>556
                     /locus_tag="ACHKNB_RS27060"
                     /old_locus_tag="pgaptmp_005412"
     CDS             222..>556
                     /locus_tag="ACHKNB_RS27060"
                     /old_locus_tag="pgaptmp_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HGU4198710.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDE"
BASE COUNT          123 a          144 c          178 g          111 t
ORIGIN      
        1 gacaaggggc gtacgaagga ccagaacgca acggctgtga acgcccgccg ccatggcgat
       61 tttgccgtgg cgctctgtat ggcaaaccgg gcgtcataca tggagggctt catcctggat
      121 gaatcagcct gtcaggcgct gccggagcgg tcgcgggcca tggagggcgg ttatcgtaat
      181 gacgatgagg catatcatga atttgatcgg gggtgctggt agtgggacgt ataattgatc
      241 ttgacggaaa acctttctcc tttgacccgg agatgcagag tgccgcgctg gatattccgc
      301 agattgccag ccgttatatt gaacatccgg cctcgggtat caccccgaac cgggcggcac
      361 agtgccttcg cggggctgaa cgtggcgatc tgattgccca gtccgatctg gcggctgaca
      421 ttgaagaaaa ggatacccac ctttttgcag agctgggcaa gcgacgtctt gccattcagg
      481 gcgtgccctg gagtattgag ccgcccccga acgccagcgc gaatgagaaa aaggacgcgg
      541 aaatgctcga cgaata
//
LOCUS       DAZFVO020000486          555 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFVO020000486 DAZFVO020000000
VERSION     DAZFVO020000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.496"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>555)
                     /locus_tag="ACHKNB_RS27065"
                     /old_locus_tag="pgaptmp_005413"
     CDS             complement(<1..>555)
                     /locus_tag="ACHKNB_RS27065"
                     /old_locus_tag="pgaptmp_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HGU4198711.1"
                     /translation="FFTLLSGNFKPDWRWDVSKETFTKEKFDSYVKPVFSKIDFYKQC
                     GVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEEMASSAYK
                     HKNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTL
                     LNSMRNMEGNKDSRINKNVVVLAG"
BASE COUNT          179 a          117 c           83 g          176 t
ORIGIN      
        1 ttccagccag aacgacgaca tttttattaa tacggctatc tttattaccc tccatatttc
       61 gcatagaatt tagcaaagta aggatatatt tatcaccaaa aatagtacta aatctatcgc
      121 ctgtatgatc cccaaggaag atacaagggg ttatcgcatc gtcctttaaa aaaacatgtt
      181 gatgcaactc attcaagatc atgtctctcg tatttaataa atcgtagagc actttattat
      241 ttttatgttt gtatgcagat gaagccatct cttcgtgttt taataattca cataataaac
      301 tccaaccctt ttccctgatg cctatatgcc ctgaaacgag caatgcataa agtacagcgc
      361 ctactcttcc atctgtatcg ccaaaataag ccgtgttggc attttgtggg ttaatcacac
      421 cacactgttt atagaagtct atttttgaaa acacaggttt aacatagcta tcaaactttt
      481 ccttagtaaa cgtctcttta gagacatccc aacgccagtc aggtttgaaa ttaccactaa
      541 gaagagtaaa gaaat
//
LOCUS       DAZFVO020000487          549 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFVO020000487 DAZFVO020000000
VERSION     DAZFVO020000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.497"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>549)
                     /locus_tag="ACHKNB_RS27070"
                     /old_locus_tag="pgaptmp_005414"
     CDS             complement(<1..>549)
                     /locus_tag="ACHKNB_RS27070"
                     /old_locus_tag="pgaptmp_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HGU4198712.1"
                     /translation="IISTPELIRYNDNIVGYGARELRVETISCWLARLVIVNKHYSHR
                     FVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTRNREYMELNRLWLHDCMPR
                     NSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHESTFYELD
                     GEWYHEICRNAIKRGGQRGEHLR"
BASE COUNT          139 a          148 c          119 g          143 t
ORIGIN      
        1 cctcagatgt tcacctcgtt gtccgcctcg tttaatggca ttcctgcaaa tctcgtgata
       61 ccactcgcca tcaagttcat agaatgttga ttcatggctg cctacataat caaaattgct
      121 tgcctggtac acgacaccaa gacagccaca acgctcatct gcaaacgact gaacccactg
      181 cacctgcgga taaagttgtc tgattagctt gagcgcgtaa ctgattgccc ttgattcgga
      241 gtttctcggc atacagtcat gcagccataa ccggttaagc tccatatatt cacggttccg
      301 cgtacccgtt acgacgcgtg caccactgtt tgggttaagg gcgtaacccc attgcattac
      361 gccaaccagc tcccgttctg aaaatatgcc cagatgaagg taggagttat ttacgaaacg
      421 gtggctgtaa tgtttattga cgatgaccag tctggccagc cagcaactta ttgtctcaac
      481 ccgcaactca cgggcgccat aaccaacgat attgtcgtta tagcgaataa gttcaggcgt
      541 gctgatgat
//
LOCUS       DAZFVO020000488          548 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFVO020000488 DAZFVO020000000
VERSION     DAZFVO020000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.498"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..476)
                     /locus_tag="ACHKNB_RS27075"
                     /old_locus_tag="pgaptmp_005415"
     CDS             complement(<1..476)
                     /locus_tag="ACHKNB_RS27075"
                     /old_locus_tag="pgaptmp_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HGU4198713.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFSFCYH"
BASE COUNT          188 a          104 c           80 g          176 t
ORIGIN      
        1 aaatgatagc aaaaagaaaa aaaaagcatt acatacttaa acagcttact gctaaagaaa
       61 tgttgttttg tagaaataat gttattttca ataatgcctg gcaatgaata ctgccaaact
      121 tcattttttt cttttacagc aaatttgaga cctaaaactt ctatttgctg attcaatgtt
      181 aacgttctat cttcaggttc ttgtgctgtt aattctctga atgttactac cagatcaggg
      241 tcggtagtaa ttattatctc aaaactacct gtaagctcgc cataaggaac gactattgtg
      301 ttttctggta actcagaaaa agcatcactt cttcctaacg aaacatcatt accaatgata
      361 acaagtagac gcccattaag tatttcatac tcgcatccat tcaatggtcc gctcaacaaa
      421 cgaatggcat aagattccaa taagtcttgt gataagaatt tatcattatt ttccatgaat
      481 accacccgct aattccataa gctctagttt agagtagtgt aacaagacgg aaatttcatg
      541 accaaatt
//
LOCUS       DAZFVO020000489          548 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFVO020000489 DAZFVO020000000
VERSION     DAZFVO020000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.499"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>548
                     /locus_tag="ACHKNB_RS27080"
                     /old_locus_tag="pgaptmp_005416"
     CDS             <1..>548
                     /locus_tag="ACHKNB_RS27080"
                     /old_locus_tag="pgaptmp_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4198714.1"
                     /translation="PSTSKSNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEG
                     TVAINRDDFEDDNLGIYSPLFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTE
                     HPVYPKVDGSGDVQMVSNMFVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKA
                     ELVAQTKVDEGRAFTDNEFVFGA"
BASE COUNT          116 a          147 c          156 g          129 t
ORIGIN      
        1 ccgtccacgt cgaaatccaa cacctacggc tggctggggc agttcccgca gttccgtgag
       61 tggatcggct cccgcgtcat tgagaagatg aaagcctatg gctatgccat cgtgaacaaa
      121 acctttgaag gcaccgtcgc cattaaccgc gatgacttcg aagacgataa cctcggtatt
      181 tattccccgt tgttccagga gatggggcgc gctgctgcgg cacaaccgga tgagctggtc
      241 tttgctgctc ttcgtgacgg gattaatgcg gcctgctatg acggccagaa cttctttgat
      301 accgaacatc ctgtttaccc caaggtggat ggctccgggg atgtgcagat ggtcagcaat
      361 atgtttgtgg cgaaaaccgg ctctgtcggc gcacaggcgg attacagcgg tcctgcctgg
      421 tatctgctcg actgttcacg cgcaatcaag ccgctgattt atcaggatcg ccgtaaggcc
      481 gagctggttg cccagaccaa agtcgatgaa ggtcgcgcat tcaccgataa cgaatttgtg
      541 tttggtgc
//
LOCUS       DAZFVO020000490          539 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFVO020000490 DAZFVO020000000
VERSION     DAZFVO020000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.500"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>539
                     /locus_tag="ACHKNB_RS27085"
                     /old_locus_tag="pgaptmp_005417"
     CDS             <1..>539
                     /locus_tag="ACHKNB_RS27085"
                     /old_locus_tag="pgaptmp_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198715.1"
                     /translation="NYLKQAWNDIKNEYEKNQTYSITLFENTLVCFMRLYNELRRKVN
                     EEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYTIQYVQNVD
                     IVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCFDDENMSIQPEQINT
                     PGISDLDTGIDLSGISFIQ"
BASE COUNT          186 a           67 c          109 g          177 t
ORIGIN      
        1 taattatttg aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata
       61 ttcaatcacg ctttttgaaa acacactggt gtgttttatg cggttataca atgaactcag
      121 acgtaaagta aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt
      181 tgaggaaatg cagaatgata atgatctatc attatttatg agaacattgc gtactaatga
      241 tacacaaatt tattcagggg tttcaggagg tattacatat actatacaat atgttcaaaa
      301 tgttgatatt gttagagtgt ccttgccggg cagaggttca gagtctatca cagattttaa
      361 aggttattat tggtatggct ttatggaata tattgaaaac attactgcgt gtgatgatgt
      421 tttttctgag tattgttttg atgatgaaaa tatgagtatc cagccagaac agataaatac
      481 gccgggaata tctgatttag atactggtat tgatttgtct ggcatatctt ttattcaga
//
LOCUS       DAZFVO020000491          527 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFVO020000491 DAZFVO020000000
VERSION     DAZFVO020000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.501"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>527
                     /locus_tag="ACHKNB_RS27090"
                     /old_locus_tag="pgaptmp_005418"
     CDS             <1..>527
                     /locus_tag="ACHKNB_RS27090"
                     /old_locus_tag="pgaptmp_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HGU4198716.1"
                     /translation="DSRHYHREWLDCAGRERKKLIDDFLSNHRAKGYLYFWCCGHIFV
                     TSILVHEDVDVGRNKRVLSDKFDEDGRVLFSVKPDRRFREGKKLNKALNILNEKLKQL
                     PSFSTWMVNKLDCYFEVFGVSNGRTVMACSSAGFYGDKGAVVVRIPVGESDNAFSPEK
                     LHPSLMAIKHSEFIA"
BASE COUNT          141 a           98 c          132 g          156 t
ORIGIN      
        1 gattcccgtc attatcacag ggaatggctt gattgcgctg gcagggagcg caagaaattg
       61 attgatgatt ttctcagtaa tcatagagct aaaggatacc tttacttctg gtgttgtggg
      121 catatttttg tcacatcaat actggttcat gaggatgttg atgtcggaag aaataagcgg
      181 gtgctgtctg ataaatttga tgaagatggc cgcgttttat tttcagttaa gcccgaccgc
      241 agattcagag agggaaaaaa actgaacaaa gcactgaata tcttaaacga gaagttaaaa
      301 cagcttccgt cattcagtac ctggatggtt aataaactgg actgctattt tgaggttttt
      361 ggtgtcagca acggccgcac agttatggcg tgctcttctg ccgggttcta tggcgacaaa
      421 ggggctgttg tggttcgtat tcctgtcggt gaatcagata atgccttttc acctgaaaag
      481 ctgcatccgt cattgatggc aatcaagcat tccgaattca tcgcaat
//
LOCUS       DAZFVO020000492          524 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFVO020000492 DAZFVO020000000
VERSION     DAZFVO020000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.502"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>524)
                     /locus_tag="ACHKNB_RS27095"
                     /old_locus_tag="pgaptmp_005419"
     CDS             complement(<1..>524)
                     /locus_tag="ACHKNB_RS27095"
                     /old_locus_tag="pgaptmp_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4198717.1"
                     /translation="QFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLIN
                     IDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYE
                     LKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSN
                     QYRYCAKHKFKQKH"
BASE COUNT          158 a          104 c           84 g          178 t
ORIGIN      
        1 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
       61 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgacac
      121 cggatagtca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
      181 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
      241 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
      301 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
      361 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
      421 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaacatc
      481 tctttcagct acaactgcgg caatttcatt agtagaaaat tgat
//
LOCUS       DAZFVO020000493          517 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFVO020000493 DAZFVO020000000
VERSION     DAZFVO020000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.503"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..494
                     /locus_tag="ACHKNB_RS27100"
                     /old_locus_tag="pgaptmp_005420"
     CDS             <1..494
                     /locus_tag="ACHKNB_RS27100"
                     /old_locus_tag="pgaptmp_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198718.1"
                     /translation="ECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITTVDGK
                     ILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDK
                     SLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRI
                     CMR"
BASE COUNT          185 a           88 c           94 g          150 t
ORIGIN      
        1 cagaatgtta ttgccttcta gcggaatgcc atatgtcttt ggcacttcat ggaaaaagtg
       61 aacttgaact tgctgctcaa aaagcattag agcttttaga ttatgtatca gacataacca
      121 ctgtcgatgg aaaaatttta gctattatgg gactgataac tggtctgtct ggacaagcaa
      181 aagtatctca tatcttattt gaacaggcta agatacactc aactgatata gcctctctct
      241 actactatag ggcactagtc cactttcata atgaaaaaat tgaagaggca aggatttgta
      301 tagacaaatc actacaactc gaacccagaa gacgaaaagc agttgtgata aaagaatgtg
      361 tagatatgta tgtgcctaac ccgctcaaaa acaatattaa actctactat aaagaaactg
      421 agagtgaaag ccatcgagtt ataattgaca acattttgaa attaaagcag ctgacgagaa
      481 tttgtatgcg ataaattttt attgacgcaa ttttatt
//
LOCUS       DAZFVO020000494          515 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFVO020000494 DAZFVO020000000
VERSION     DAZFVO020000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.504"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>515
                     /locus_tag="ACHKNB_RS27105"
                     /old_locus_tag="pgaptmp_005421"
     CDS             <1..>515
                     /locus_tag="ACHKNB_RS27105"
                     /old_locus_tag="pgaptmp_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HGU4198719.1"
                     /translation="LYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDK
                     FDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPC
                     PNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDN
                     HGKEITNRKGLP"
BASE COUNT          186 a           85 c          108 g          136 t
ORIGIN      
        1 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
       61 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      121 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      181 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      241 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      301 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      361 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      421 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      481 cacgggaagg aaataacaaa cagaaaagga ttacc
//
LOCUS       DAZFVO020000495          499 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFVO020000495 DAZFVO020000000
VERSION     DAZFVO020000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.505"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(152..>499)
                     /locus_tag="ACHKNB_RS27110"
                     /old_locus_tag="pgaptmp_005422"
     CDS             complement(152..>499)
                     /locus_tag="ACHKNB_RS27110"
                     /old_locus_tag="pgaptmp_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGU4198720.1"
                     /translation="KGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVI
                     QQGITGKELSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQD
                     QKHCCKVLLTFSE"
BASE COUNT          147 a          101 c           97 g          154 t
ORIGIN      
        1 tgaaatccat aaattcaagc gcagtgccca gccatcccga tactgctgct ttcaccaaat
       61 ccttagtgct tctttcatgt ttttctattg tcataatggt tatctctaaa aaagaggtaa
      121 gatgcgtact acttactcgc cgttattggt attattcgga aaaagtgagt aagactttgc
      181 agcaatgttt ttgatcctgt tcaaataaac taatggcatc agcaacatgc tggaaatcaa
      241 acgtatgggt aattaatttt tctggtttaa ttaacccttt acttaaccag tcgataacaa
      301 ccggaaattt atttgcattt aagcgtgaag agaaaataga gagttctttt ccggtaattc
      361 cttgctgaat cacttcagac ggttcactgg agaagcccat caatacaata cgtgccgctg
      421 gagaagccag cgttacggcc tctttcagga tagaaggatg acaagccgca tcgataatta
      481 atgtcggctt gatgccttt
//
LOCUS       DAZFVO020000496          490 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFVO020000496 DAZFVO020000000
VERSION     DAZFVO020000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.506"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            69..461
                     /gene="ydeI"
                     /locus_tag="ACHKNB_RS27115"
                     /old_locus_tag="pgaptmp_005423"
     CDS             69..461
                     /gene="ydeI"
                     /locus_tag="ACHKNB_RS27115"
                     /old_locus_tag="pgaptmp_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HGU4198721.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          147 a          103 c          117 g          123 t
ORIGIN      
        1 ttatttggca attataaaaa tagcaaccag acttaataag tcaaaccaaa cgcaattaag
       61 gagtcagtat gaaatttcag gccattgtat tagcaagttt tcttgtcatg ccttatgcgt
      121 tggcagatga ccagggcggt ttaaaacagg atgcagcgcc accgccgcct catgcaatag
      181 aagatggcta tcgcggaacc gatgatgcaa aaaaaatgac cgttgatttc gcaaatacca
      241 tgcacgatgg cgcttcggtt tcactacgcg gtaatttgat ttctcacaaa ggagaggacc
      301 gttacgtttt tcgcgataag agcggtgaaa ttaatgtcgt tattcctgcg gccgtctttg
      361 atggacgaga agttcagccg gaccagatga tcaacattag cggcagtctg gataagaaaa
      421 gcgcgccggc cgtcgttcgg gtcactcatt tgcagaaata aaaataaatt agcctgatgg
      481 cctgaactac
//
LOCUS       DAZFVO020000497          481 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFVO020000497 DAZFVO020000000
VERSION     DAZFVO020000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.507"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>481
                     /locus_tag="ACHKNB_RS27120"
                     /old_locus_tag="pgaptmp_005424"
     CDS             <1..>481
                     /locus_tag="ACHKNB_RS27120"
                     /old_locus_tag="pgaptmp_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HGU4198722.1"
                     /translation="KKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKMGKIDHE
                     IDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLLPPSNAF
                     SQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGMFADLE"
BASE COUNT          159 a          114 c          112 g           96 t
ORIGIN      
        1 ggaaaaagct ggagcaggca ttccgacgcg aacatgccga aacgggaata acattactgg
       61 actggtgccg gaagaaaaag attaattaca acaccgccag aacccgtata aaaatgggca
      121 aaatcgatca tgaaattgat cataaaaccg atcatgaaat cgatcatgac atctcagatg
      181 aagaacccag caatgacgcg tgttccgacg atgaaaaatg cgcaaaaaac tctgaaaaaa
      241 actgcgcaaa ttcggcagaa acgaaacgga ttcgtggttc ccgactttta cctccttcaa
      301 acgctttttc tcagcgaaac acccacgccg taagacaccg tggatatgcg aagtatcttg
      361 aggcagataa cctcatggat gatgcgtccg acatggtgct gttcgatgaa ctggtgttca
      421 cccgggcccg cgcactttca gtaactaagg cacttaaagg gatgttcgcc gacctggaag
      481 a
//
LOCUS       DAZFVO020000498          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFVO020000498 DAZFVO020000000
VERSION     DAZFVO020000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.508"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            119..479
                     /locus_tag="ACHKNB_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /pseudo
     CDS             119..479
                     /locus_tag="ACHKNB_RS27125"
                     /old_locus_tag="pgaptmp_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          136 a          107 c          119 g          117 t
ORIGIN      
        1 cgccgtattc tggtcatttg ttgacaccct gatataccca atcagcatac gatttcctta
       61 ttataaaaag ccggaattgt cgcatgagga ataaaacaga gttaatcctg gctttcctga
      121 aaacctcggt ttacgggaaa cggtaaacag ggcggcgaat gctctgccat cggacggcac
      181 cgccgttgcc gcgaatagac tcgccacccc aagaaatatt aatggggttc cctttgacgg
      241 gacgcaggac ataaacatca cttctggtat aacacagcag gatgcggatt cccgatatat
      301 ccaggatatc aggctgggag cagagaacag gcaggtaatg tacggtgggg catattactc
      361 tgcgtacggt aatgttatgg caggcctgca gattgacagt aaagttgatg cttcaaacga
      421 ttttatagta ttcagacccc tgcaaaaatt agtcggaggt acatggatta cggtatctc
//
LOCUS       DAZFVO020000499          479 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFVO020000499 DAZFVO020000000
VERSION     DAZFVO020000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.509"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..479
                     /locus_tag="ACHKNB_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /pseudo
     CDS             1..479
                     /locus_tag="ACHKNB_RS27130"
                     /old_locus_tag="pgaptmp_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          114 a          138 c          138 g           89 t
ORIGIN      
        1 gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga ttgcacgtct
       61 caaacgccag ctggcagaac gggatgaaga gctggctatc ctccaaaagg ccgcgacata
      121 cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc
      181 atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag
      241 cggcggacaa ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc
      301 gcggctttta cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt
      361 gctcagggtt acccctttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg
      421 agggcaaagg cctcccggaa gttcagcccg gtcagctacc gcgcacacgg cctgcctgt
//
LOCUS       DAZFVO020000500          477 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFVO020000500 DAZFVO020000000
VERSION     DAZFVO020000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.510"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>477
                     /locus_tag="ACHKNB_RS27135"
                     /old_locus_tag="pgaptmp_005427"
     CDS             <1..>477
                     /locus_tag="ACHKNB_RS27135"
                     /old_locus_tag="pgaptmp_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198723.1"
                     /translation="AGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLR
                     VTFGVQSLVETTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNN
                     LPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFG"
BASE COUNT          109 a          131 c          153 g           84 t
ORIGIN      
        1 tgccggggag caggagcaga caccgccgga aggctttgag tcctccgggg cggaaaccgc
       61 actgggcgtg gaggtgacga aggcaaagcc ggtgacgcgc accatcacgt cagcgaacat
      121 tgaccgtctg cgggtcacct tcggggtgca gtcactggtg gagaccacct caaagggtga
      181 ccgtaatccc tcttctgtcc gcctgctgat tcagcttgag cgtaacgggc actgggtgac
      241 ggagaaggat gtcaccatta acggcaagac cacctcgcag tacctgacgt cggtgattct
      301 gaataatctc cctgagcgcc cctttaacat ccggatggtc agggagacgg cggacagcac
      361 cacggaccag ctgcagaaca gaacgctgtg gtcgtcatac accgaaatca tcgatgtgaa
      421 acagtgctac ccgaacacgg cgattgtggg gctgcaggtg gatgcggagc agtttgg
//
LOCUS       DAZFVO020000501          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFVO020000501 DAZFVO020000000
VERSION     DAZFVO020000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.511"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..195
                     /locus_tag="ACHKNB_RS27140"
                     /old_locus_tag="pgaptmp_005428"
     CDS             <1..195
                     /locus_tag="ACHKNB_RS27140"
                     /old_locus_tag="pgaptmp_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198724.1"
                     /translation="EVAHHEVITFPSLDGAQPTIPGMNIVPDGAVILNIRSQAYKITA
                     VTYGFLQVHNGSQWVTIKTE"
     gene            259..>476
                     /locus_tag="ACHKNB_RS27145"
                     /old_locus_tag="pgaptmp_005429"
     CDS             259..>476
                     /locus_tag="ACHKNB_RS27145"
                     /old_locus_tag="pgaptmp_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198725.1"
                     /translation="MIIKNLKHYTPEKPDVPGAMYLKSEDGQDWYECQSLFSAETLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVT"
BASE COUNT          137 a           98 c          118 g          123 t
ORIGIN      
        1 gaagtcgcac accatgaggt gattactttc ccgtcactgg atggagcaca accaacgatt
       61 cccggcatga atatagtccc tgatggtgct gtaattctga atatccggtc acaggcttac
      121 aaaattactg ctgtaactta tggctttctt caggttcaca atggaagcca gtgggtaacg
      181 attaaaacgg aataacgtga ctcacactgg ttgttgcgtt ccattatgcc caaccgggca
      241 ttaaatacgg agaataatat gattattaaa aacctgaagc actatacccc tgaaaaacct
      301 gatgtgccgg gtgcgatgta cctgaaatca gaggatggtc aggactggta tgagtgtcag
      361 tcgttatttt cagcggagac gctgaagctg gtttatgaca gtaacggtat aatcaccagc
      421 ataagtaagg atgtctctat gctgtggccg gtaaaccaga gtgtggcaga ggttac
//
LOCUS       DAZFVO020000502          476 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFVO020000502 DAZFVO020000000
VERSION     DAZFVO020000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.512"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>476
                     /locus_tag="ACHKNB_RS27150"
                     /old_locus_tag="pgaptmp_005430"
     rRNA            <1..>476
                     /locus_tag="ACHKNB_RS27150"
                     /old_locus_tag="pgaptmp_005430"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZFVO020000503          473 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFVO020000503 DAZFVO020000000
VERSION     DAZFVO020000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.513"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            193..>473
                     /locus_tag="ACHKNB_RS27155"
                     /old_locus_tag="pgaptmp_005431"
     CDS             193..>473
                     /locus_tag="ACHKNB_RS27155"
                     /old_locus_tag="pgaptmp_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4198726.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMA"
BASE COUNT          111 a          115 c          128 g          119 t
ORIGIN      
        1 gcaaggaata aacaacaaaa cgtgaagtaa tatttcagct ggcgaataat acccgacagt
       61 aatcactctg cgcaactgcg cggccttttt cgtattgcgg gctgtagtct tccttctgtc
      121 attgtccttt aacttccgga cctcagcccg ctctttatct gattcagtac actatcccgg
      181 ccgggaggat tcatgacatt taaacactat gacgtggtca gggcggcgtc gccgtcagac
      241 cttgcggatg cgcttgctca aaaaattcgt gaaggatggc agccatacgg cgggccgttt
      301 tcttcgtata cggatgatgg cgcagcactt attcaggcga ttgtcgcaga aggtgatgtg
      361 agcacacctg tgttggtgaa gccgtcggat ggagaaggca cagttatcag caccaccagc
      421 gaaccggagt attactttgt tgtggttctg gcggggcagt caaacagcat ggc
//
LOCUS       DAZFVO020000504          472 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFVO020000504 DAZFVO020000000
VERSION     DAZFVO020000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.514"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(81..>472)
                     /locus_tag="ACHKNB_RS27160"
                     /old_locus_tag="pgaptmp_005432"
     CDS             complement(81..>472)
                     /locus_tag="ACHKNB_RS27160"
                     /old_locus_tag="pgaptmp_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HGU4198727.1"
                     /translation="LVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNARLN
                     SQQRQIRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRF
                     NEQTAAKAGVAASDGDVSYNVGVNFEF"
BASE COUNT          110 a          145 c          103 g          114 t
ORIGIN      
        1 cacctgtttc atgctcacat gagaaaaaac ggcaggcaaa actccacgcc tgccgtcatg
       61 cttcatgtca ctgtcagcaa ttaaaactca aagttcacac ctacgttgta ggacacatcg
      121 ccatcactcg ctgccacacc tgctttcgct gctgtctgct cattgaaacg gtaaccaaca
      181 cccacggcaa cagcctgttt atcactgtac ccccccaggg cagcagtggc gttgaatttc
      241 cccacactgt acggctggaa aagccccgcc agcgctgcac tctgtgctgc tgcacgcttc
      301 atctcttcgt gattctcacg aatttgacgc tgctggctgt tcaggcgtgc attatgttcc
      361 tgcaactggc gagtattgga ctgaatagcc tgtgtatttt tagtaacccg gcggtcaacg
      421 ctaccaactg cagcatcaat acggtctgtt ctgccggaca gagcaacgag ac
//
LOCUS       DAZFVO020000505          468 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFVO020000505 DAZFVO020000000
VERSION     DAZFVO020000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.515"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            197..>468
                     /locus_tag="ACHKNB_RS27165"
                     /old_locus_tag="pgaptmp_005433"
     CDS             197..>468
                     /locus_tag="ACHKNB_RS27165"
                     /old_locus_tag="pgaptmp_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HGU4198728.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYFVVVLAGQSNGMS"
BASE COUNT          110 a          126 c          126 g          106 t
ORIGIN      
        1 gcaaggaata aacaacaaaa cgtgaagtaa tcaatttttc agctggcgaa taatacccga
       61 cagtaatcac tctgcgcaac tgcgcggcct ttttcgtatt gcgggctgta gtcttcctcc
      121 agtcattgtc ctgtaacttc cggacttcag cccgcccctt atctgactca caacattatc
      181 ccggccggga ggattcatgg catttaaaca ctatgacgtg gtcagggcgg cgtcgccgtc
      241 agaccttgcg gagcgactga ctcaaaaact gaaggagggg tggcagccat ttggcagccc
      301 tgtcgccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg atgtcaccac
      361 acctgtggtg gtgaagccgt cggatggaga aggcacagta atcagcgcca ccagcgaccc
      421 ggagtattac tttgttgtgg ttctggcagg gcagtcaaac ggcatgtc
//
LOCUS       DAZFVO020000506          463 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFVO020000506 DAZFVO020000000
VERSION     DAZFVO020000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.516"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..122)
                     /locus_tag="ACHKNB_RS27170"
                     /old_locus_tag="pgaptmp_005434"
     CDS             complement(<1..122)
                     /locus_tag="ACHKNB_RS27170"
                     /old_locus_tag="pgaptmp_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198729.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLI"
     gene            140..331
                     /gene="yfgG"
                     /locus_tag="ACHKNB_RS27175"
                     /old_locus_tag="pgaptmp_005435"
     CDS             140..331
                     /gene="yfgG"
                     /locus_tag="ACHKNB_RS27175"
                     /old_locus_tag="pgaptmp_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HGU4198730.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          121 a          117 c           81 g          144 t
ORIGIN      
        1 ataattaaat taacttccat aacattaaaa tatgtatcca ctgacgcttt tttacataac
       61 gaagaattga ccattttgtc ctgttgtgcc ttaatgtaag taccgtccac agcgtgggac
      121 atacttcaag gaaccttttg tgagtcaggc aaccagtatg cgaaaacgac accgatttaa
      181 cagtcgcatg acccgtatcg tactgctcat cagctttatc ttcttctttg gccgttttat
      241 ctactcgtcc gtcggtgcct ggcagcacca tcagagcaaa aaagaagctc agcaatccac
      301 actctccgtc gaatcaccgg tacaacgtta gcggttacct tctccacttt cacagaacct
      361 aacggcactt cgctgtcgga tgcttttgct ctttggaatt atcaaagcgg cagatattct
      421 ttcatcttaa attttacgtc tttgtcctga ctgatgttta tcc
//
LOCUS       DAZFVO020000507          462 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFVO020000507 DAZFVO020000000
VERSION     DAZFVO020000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.517"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            278..397
                     /locus_tag="ACHKNB_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /pseudo
     CDS             278..397
                     /locus_tag="ACHKNB_RS27180"
                     /old_locus_tag="pgaptmp_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          122 a          120 c           75 g          145 t
ORIGIN      
        1 acataaaatg cgaattattt agtacaaaaa gtgaactttt gatgatcttt ccctaatctc
       61 acataggatc agatctcgtc tgatcgttcc ccctgcgctc actaccatgg ctctttacca
      121 cagcagccgg ggttgtttgg gaccgacttc cgacagcaga tcccggtggg cctgccacca
      181 tctcttcaca agcttatgct tattacatgc aaaacgtccc gttttgcatc tttgtgccta
      241 cggcacaccg atgatctttc cctgtgatct ttccctgaat ctgcccctga gaacctgaat
      301 caaacgtctg actcgcagaa ccatctgttt ctcgcgctct gtagaacttc atgaaaaagt
      361 catcggtgct tttatcgaaa aatacatgtt ctactaattg aagtcatcat caaaaaattt
      421 aattaatcga atcataccta taacctttta attttacttt tt
//
LOCUS       DAZFVO020000508          455 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFVO020000508 DAZFVO020000000
VERSION     DAZFVO020000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.518"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>455
                     /locus_tag="ACHKNB_RS27185"
                     /old_locus_tag="pgaptmp_005437"
     CDS             <1..>455
                     /locus_tag="ACHKNB_RS27185"
                     /old_locus_tag="pgaptmp_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HGU4198731.1"
                     /translation="AYGYAIVNKTFEGTVAINRDDFEDDNLGIYSPLFQEMGRAAAAQ
                     PDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNMFVAKTGSVGAQA
                     DYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEFVFGA"
BASE COUNT          101 a          118 c          124 g          112 t
ORIGIN      
        1 gcctatggct atgccatcgt gaacaaaact tttgaaggca ccgtcgccat taaccgcgat
       61 gacttcgaag acgataacct cggtatttat tccccgttgt tccaggagat ggggcgcgct
      121 gctgcggcac aaccggatga actggtcttt gctgctcttc gtgacgggat taatgcggcc
      181 tgctatgacg gccagaactt ctttgatacc gaacatcctg tttaccccaa agtggatggc
      241 tccggggatg cgcagatggt cagcaatatg tttgtggcaa aaaccggctc tgtcggtgca
      301 caggcggatt acagcggtcc tgcctggtat ctgctcgact gttcacgcgc aatcaaaccg
      361 ctgatttatc aggatcgccg taaggctgaa ctggttgccc agaccaaagt cgatgaaggt
      421 cgcgcattca ccgataacga atttgtgttc ggtgc
//
LOCUS       DAZFVO020000509          451 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFVO020000509 DAZFVO020000000
VERSION     DAZFVO020000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.519"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>451
                     /locus_tag="ACHKNB_RS27190"
                     /old_locus_tag="pgaptmp_005438"
     CDS             <1..>451
                     /locus_tag="ACHKNB_RS27190"
                     /old_locus_tag="pgaptmp_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198732.1"
                     /translation="NLKEIADAGAEAQRLARGYLGLGALATKNSLGPGDVNALAKDQN
                     LADLENAGTARNNLDVYSKSEGDNRYLRREQNGADIPDKGAFIDNVGLRETVNKAADA
                     LPSGGTAVAANRLATPRNINGVPFDGTLDINITSGMTQSTADGRYVQN"
BASE COUNT          120 a          109 c          128 g           94 t
ORIGIN      
        1 aacctgaaag aaattgctga tgccggtgcg gaagcccagc gtcttgcccg tggttatctt
       61 ggtcttgggg cgcttgccac aaaaaacagt cttggtcccg gtgacgttaa tgctctggcg
      121 aaggatcaga accttgccga cctggagaat gcgggaaccg cccgtaataa tctggatgtt
      181 tacagcaaaa gcgaaggtga taaccgttac ctgcgcaggg agcagaacgg cgcagacatt
      241 ccggataaag gcgcttttat cgataacgtc ggtttacggg aaacggtaaa taaggctgct
      301 gatgccctgc catcgggtgg aaccgccgtg gcagcaaaca gactcgccac cccaagaaat
      361 attaatggtg ttccttttga cggaacgctg gatatcaaca tcacgtcagg aatgacgcag
      421 tcaaccgcag atggccggta tgtacagaat g
//
LOCUS       DAZFVO020000510          447 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFVO020000510 DAZFVO020000000
VERSION     DAZFVO020000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.520"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..113
                     /locus_tag="ACHKNB_RS27195"
                     /old_locus_tag="pgaptmp_005439"
     CDS             <1..113
                     /locus_tag="ACHKNB_RS27195"
                     /old_locus_tag="pgaptmp_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198733.1"
                     /translation="TDLAEAEAEAEAEAEAEAGQDAPRKKTGNKAKQARA"
     gene            110..>447
                     /locus_tag="ACHKNB_RS27200"
                     /old_locus_tag="pgaptmp_005440"
     CDS             110..>447
                     /locus_tag="ACHKNB_RS27200"
                     /old_locus_tag="pgaptmp_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HGU4198734.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNG"
BASE COUNT           99 a          125 c          137 g           86 t
ORIGIN      
        1 gtacagattt agccgaagcc gaagccgaag ccgaagccga agccgaagcc gaagccggac
       61 aggatgctcc ccggaaaaag accggtaaca aagcgaaaca ggcccgtgca tgaattacgc
      121 cactgaaacc gatatgcggg cgcgttaccg cgaggatttg ctcaggccgt tactggctgt
      181 gccccgttcg gatgagccgg atacgcgcaa gctgaaccgg gcgctgacag atgcgtcagc
      241 ccttatcgac agctatctgt ccgcccgtta cacgctgccg ctggaggtta ttccggccgt
      301 tcttgttcag cactgttgcg cgattgcctt ttattacctg tgcgatcagc gagcctcaga
      361 tcaggcgcgt gaccgttacc gtgaggcgct ggcctggctg aaagatgtca tgaacggcaa
      421 tgtgccggta ggtgtggata cgaacgg
//
LOCUS       DAZFVO020000511          438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFVO020000511 DAZFVO020000000
VERSION     DAZFVO020000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.521"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            133..>438
                     /locus_tag="ACHKNB_RS27205"
                     /old_locus_tag="pgaptmp_005441"
     CDS             133..>438
                     /locus_tag="ACHKNB_RS27205"
                     /old_locus_tag="pgaptmp_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HGU4198735.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     "
BASE COUNT          148 a           84 c           60 g          146 t
ORIGIN      
        1 tatgcataac tttatttaca atttaaggta taaaccctat tccctcaatg ttaagcaaat
       61 ataacaatca ctcttatttt aaattttaca cgtaatattt accctaaatg attttagagt
      121 acaggtgggc atatgcaagt cttttccagt gatgtctact tcactgtagg cactaatgca
      181 ttattagcat cccaaaaaga atattacagc gatttagttg cgttggttga tcttggacat
      241 tcgttcgttg ttattgatga gcatcaacat cgaaatctca aaccaaatac agaacctgta
      301 aacatccttt tgagcaacaa tttcatcagg atcaataaga atattacatt atctgattta
      361 acgcatttcc tggtttcaaa tcttcataca caaaatgtgt attccacaca agaaccactt
      421 acccatgatg agatagat
//
LOCUS       DAZFVO020000512          438 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFVO020000512 DAZFVO020000000
VERSION     DAZFVO020000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.522"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..329
                     /locus_tag="ACHKNB_RS27210"
                     /old_locus_tag="pgaptmp_005442"
     CDS             <1..329
                     /locus_tag="ACHKNB_RS27210"
                     /old_locus_tag="pgaptmp_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198736.1"
                     /translation="KTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTYS
                     VTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSAE
                     FEQVVN"
     gene            329..>438
                     /locus_tag="ACHKNB_RS27215"
                     /old_locus_tag="pgaptmp_005443"
     CDS             329..>438
                     /locus_tag="ACHKNB_RS27215"
                     /old_locus_tag="pgaptmp_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HGU4198737.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERY"
BASE COUNT           96 a          101 c          146 g           95 t
ORIGIN      
        1 tgaaaacctt ccgctggaaa gtgaaacccg gtatggatgt ggcttcggcc ccttctgtaa
       61 gaaaggtgcg ctttggtgat ggctattccc agcgagcgcc tgccgggctg aatgccaacc
      121 tgaaaacgta cagcgtgacg ctttctgtcc cccgtgagga ggccacggta ctggagtcgt
      181 ttctggaaga gcacgggggc tggaaagcct ttctgtggac gccgccttat gagtggcggc
      241 agataaaggt gacctgcgca aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca
      301 gcgcagagtt tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa cactgaatga
      361 atgcacccgt gcggagcagt cggccagcgt ggtgctctgg gaaatcgacc tgacagaggt
      421 cggtggagaa cgttattt
//
LOCUS       DAZFVO020000513          437 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFVO020000513 DAZFVO020000000
VERSION     DAZFVO020000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.523"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>437
                     /locus_tag="ACHKNB_RS27220"
                     /old_locus_tag="pgaptmp_005444"
     CDS             <1..>437
                     /locus_tag="ACHKNB_RS27220"
                     /old_locus_tag="pgaptmp_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4198738.1"
                     /translation="PDCPWQHIVFTLPCQYWSLVFHNRWLLAEMSRIAADVIQEICLQ
                     ADVVPGIFTVIHTWGRDQQWHPHIHLSTTAGGVTPDHTWKNLHFYARKVMSMWRYRIT
                     RLLSRKYPELVIPDALAVEGSSRRDWNRFLDSHYRRGWNVNVS"
BASE COUNT           97 a          117 c          130 g           93 t
ORIGIN      
        1 ttcccgactg tccgtggcag catattgtgt tcacacttcc ctgccagtac tggtccctgg
       61 tgttccacaa ccggtggtta ctggcagaga tgagccgcat tgctgcggat gtgatacagg
      121 aaatctgcct ccaggcagat gtggtgccgg ggatattcac ggtgatccac acatggggac
      181 gtgaccagca gtggcatccg cacattcacc tgtcgacaac ggccggcggc gtgacaccag
      241 accacacctg gaaaaacctt catttttacg cccgtaaggt gatgagcatg tggcgttacc
      301 ggataacacg gttgctgtca cggaaatacc cggagctggt gataccggat gcgctggcag
      361 ttgaaggaag cagcagacgg gactggaatc gcttcctgga cagtcattac cggcggggct
      421 ggaatgtcaa cgtatcc
//
LOCUS       DAZFVO020000514          418 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFVO020000514 DAZFVO020000000
VERSION     DAZFVO020000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.524"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(198..>418)
                     /locus_tag="ACHKNB_RS27225"
                     /old_locus_tag="pgaptmp_005445"
     CDS             complement(198..>418)
                     /locus_tag="ACHKNB_RS27225"
                     /old_locus_tag="pgaptmp_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HGU4198739.1"
                     /translation="GKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHV
                     RQRTMKKELEKLTSADEILAYRVGWGDP"
BASE COUNT           95 a          119 c           76 g          128 t
ORIGIN      
        1 actctcatcc ggcatgctgt gtcacatctg cttacactgt aatttatgag aaaaatctca
       61 atagcagtaa aaagtaactg ttcagttaaa gtgtaatatt tacctaagta acttttcctg
      121 tgtattaccg tgaaggagca taattggtta ttgggcgttc tccttccata aaaatacgca
      181 gtgtcctcct ttccagatta cggatcaccc cagccaaccc tgtatgccag tatctcatcc
      241 gcactggtca gcttttccag ctctttcttc atggtgcgct gccggacgtg gatttccatc
      301 cctttggtga acatcgcctg ctctgccgct tcactcagcg ctataagctc ttctgctgtc
      361 acctccacat cattgttttc cgcatccgtc cagaaaaacg cctccggcag tttccccg
//
LOCUS       DAZFVO020000515          417 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFVO020000515 DAZFVO020000000
VERSION     DAZFVO020000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.525"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            281..>417
                     /locus_tag="ACHKNB_RS27230"
                     /old_locus_tag="pgaptmp_005446"
     CDS             281..>417
                     /locus_tag="ACHKNB_RS27230"
                     /old_locus_tag="pgaptmp_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HGU4198740.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SA"
BASE COUNT          132 a           83 c           78 g          124 t
ORIGIN      
        1 aaaactaact atataaataa ccataaccat gccgttgtgt tcgccctctg actagcaact
       61 ctggttttct ttcttagctg aataatctgg ttatagcgtg aggactgacc gttaaatttc
      121 tccatcttac tggataacac accatgagct gctaccttta aaacacctga atgtcaacag
      181 gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta aggtcatata
      241 tagtcttatc gataacatgg ttaattttaa ggataagaat atgcctgctg taatagataa
      301 agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt cgatgaatga
      361 aagcacagcg aagggaatct ttaaatattt aaaagaactg ggagtacccg cgagtgc
//
LOCUS       DAZFVO020000516          416 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFVO020000516 DAZFVO020000000
VERSION     DAZFVO020000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.526"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>416)
                     /locus_tag="ACHKNB_RS27235"
                     /old_locus_tag="pgaptmp_005447"
     CDS             complement(<1..>416)
                     /locus_tag="ACHKNB_RS27235"
                     /old_locus_tag="pgaptmp_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198741.1"
                     /translation="IGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQASGTFEQNNHVVI
                     NNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGG"
BASE COUNT           83 a          146 c          100 g           87 t
ORIGIN      
        1 ccaccatcac gcatctgtgt ctgaatttca tcacgggcac ccttgcgggc catgtcatac
       61 accgccttca gagcagccgg acctatctgc ccgttcgtgc cgtcgttgtt aatcaccaca
      121 tggttattct gctcaaacgt cccggacgcc tgcgaccggc tgtcagccag actgcccggt
      181 gtaccgacat aaccaccggt ggcatagccg cgcatcagcc ggtagagatt ccccacgcca
      241 atccggctgg ttgcctcctt tgtgaagaca aattcaccac ggtgaacaat ccccgctggc
      301 tcatatttgc cgccggttcc cgtaaatcct ccggttgcaa aatggaattt cgccgcagcg
      361 gcctgaatgg ctgtaccgcc tgacgcggat gcgccgccac caacagcccc gccaat
//
LOCUS       DAZFVO020000517          411 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFVO020000517 DAZFVO020000000
VERSION     DAZFVO020000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.527"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..391
                     /gene="espX1"
                     /locus_tag="ACHKNB_RS27240"
                     /old_locus_tag="pgaptmp_005448"
     CDS             <1..391
                     /gene="espX1"
                     /locus_tag="ACHKNB_RS27240"
                     /old_locus_tag="pgaptmp_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HGU4198742.1"
                     /translation="FSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLV
                     NKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELKKFMHDYD
                     KTSPEMRKYLQSINESMFLMKNIAHQN"
BASE COUNT          163 a           74 c           64 g          110 t
ORIGIN      
        1 cttttcaaat ggagaaatta ataatctctt cattaaaaaa agcaacatat ttggagcatc
       61 attcactaat accagaataa aaaatttgct ctgtgaagat attatgcctg gtcgatggac
      121 cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca aaaccctggc
      181 aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac aaactttagt
      241 tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa ctgaaaaagt ttatgcacga
      301 ttacgacaaa acatccccgg aaatgagaaa ataccttcag agcatcaacg agtctatgtt
      361 ccttatgaaa aacatagctc atcaaaatta aaaaatctca aaaaagaagt a
//
LOCUS       DAZFVO020000518          408 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFVO020000518 DAZFVO020000000
VERSION     DAZFVO020000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.528"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..340
                     /locus_tag="ACHKNB_RS27245"
                     /old_locus_tag="pgaptmp_005449"
     CDS             <1..340
                     /locus_tag="ACHKNB_RS27245"
                     /old_locus_tag="pgaptmp_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198743.1"
                     /translation="DWRTRYLPRVARLADLGENNSLMNPQLARVGGLATSSLRTAQMA
                     QDNQMARYGVSRPDNPDSNTLGLRNALAIAGAKNGIREAEQDRQMNILTGASAPARQK
                     LSVGGQLVAA"
BASE COUNT          106 a           94 c          130 g           78 t
ORIGIN      
        1 cgactggcgc accagatatt taccccgtgt agcaaggctg gctgaccttg gggagaacaa
       61 cagtctgatg aatccacagc ttgcacgggt gggaggcctt gccacttcca gtctccgtac
      121 agcgcagatg gcgcaggata accagatggc gagatacggg gtaagccgcc cggataatcc
      181 cgacagtaat acgctggggt tacgtaatgc cctggcaata gcaggtgcga aaaatggtat
      241 ccgtgaagcc gaacaggatc gccagatgaa tattctgacg ggggcttctg caccggcaag
      301 acagaaactg agtgttggcg gacaactggt ggcagcgtaa gggggcaata tgggatacgg
      361 tttactggat attgcaaatc agtcgcggcg tgaggcatta cagggaat
//
LOCUS       DAZFVO020000519          399 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFVO020000519 DAZFVO020000000
VERSION     DAZFVO020000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.529"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            73..291
                     /gene="ymgF"
                     /locus_tag="ACHKNB_RS27250"
                     /old_locus_tag="pgaptmp_005450"
     CDS             73..291
                     /gene="ymgF"
                     /locus_tag="ACHKNB_RS27250"
                     /old_locus_tag="pgaptmp_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HGU4198744.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          101 a           74 c           86 g          138 t
ORIGIN      
        1 tcttgttact attgttcatt attttgtttg ctataattgt ttgaaagttt tgacaggatt
       61 gccattagta gcatgaacaa tagtaataat ctggattatt tcactctcta tatcatcttt
      121 tccattgcat ttatgctgat caccctcctg gtcatcctta ttgcaaaacc cagtaccggg
      181 ctgggagaag tgcttgtgac gataaatttg cttaatgccc ttgtttggct ggcgatcaat
      241 ctggttaatc gattaaggga aagactcgtc agccacaggg atcagcaata atctttcagt
      301 ttctcactgt cagtatgcgg ctgaatgggt tgctggcagt gaatgcctgg atcattgaag
      361 taaaggaatt attgcgcaaa tagttgtcaa ccctggtgt
//
LOCUS       DAZFVO020000520          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZFVO020000520 DAZFVO020000000
VERSION     DAZFVO020000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.530"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..279
                     /locus_tag="ACHKNB_RS27255"
                     /old_locus_tag="pgaptmp_005451"
     CDS             <1..279
                     /locus_tag="ACHKNB_RS27255"
                     /old_locus_tag="pgaptmp_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198745.1"
                     /translation="EDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQ
                     HSEGQQSVVRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
BASE COUNT           83 a          110 c          123 g           75 t
ORIGIN      
        1 gaagacaata atcaggtgct tgtgcgtgag cgggatgagg cgcgtcaggt gctggcaaat
       61 cagcagcaca ccctgcaact catttcacaa atcagtgagg cggccacgaa tgaaaaacaa
      121 cagaacattc agcacagcga ggggcagcag agcgttgtcc gccggtcgct ggcagcagtg
      181 cctgcggcct ctgcccctgt tcctgatgat gtggctgatc gggtgcgccg ggccgtctgt
      241 gaaatacgtg cctgtgaagc cggtgccgat ccccgctgaa tggctggctg actgcctggt
      301 ccctcctgcg ccggagccgt tcacgtttgg ggcatcggtc acttacaacc tgcaactgct
      361 ggcggtgatc aagaactgca acgtggacaa g
//
LOCUS       DAZFVO020000521          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZFVO020000521 DAZFVO020000000
VERSION     DAZFVO020000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.531"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(120..>391)
                     /locus_tag="ACHKNB_RS27260"
                     /old_locus_tag="pgaptmp_005452"
     CDS             complement(120..>391)
                     /locus_tag="ACHKNB_RS27260"
                     /old_locus_tag="pgaptmp_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HGU4198746.1"
                     /translation="GISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLISLNPEPKKKEYAVFFI"
BASE COUNT           89 a          117 c           95 g           90 t
ORIGIN      
        1 tcatcctcac tgaaggattt caccgtcagc agggagcagg cccgtttaag agtcacatca
       61 gcctcctgaa aataaaaaaa ccgccgcagc ggtccatgat ggttacaggg tgaacagggt
      121 tatatgaaaa aaaccgcata ttctttcttt ttgggctccg gattcaggga tatcagggat
      181 accgcattga acagcgccat cagcgggtca attttccccc gtccgctggc ctgtttggta
      241 ataaggatgg cgttaccttt aggctccacc cgggcattgc caacgcacca ggccatcagt
      301 ggctgaccac catgcaccag cactccctca gccagtttgc gctcggtggt tttgatggcc
      361 ccgcccagct tccagccctg gcttatcccc a
//
LOCUS       DAZFVO020000522          391 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZFVO020000522 DAZFVO020000000
VERSION     DAZFVO020000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.532"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            41..340
                     /locus_tag="ACHKNB_RS27265"
                     /old_locus_tag="pgaptmp_005453"
     CDS             41..340
                     /locus_tag="ACHKNB_RS27265"
                     /old_locus_tag="pgaptmp_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGU4198747.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMLRKEVV"
BASE COUNT           92 a           77 c          104 g          118 t
ORIGIN      
        1 tttgctccgg aaaaatgttt aagcgtctgg gaggtgtggc gtgaactgtt ttcagtttgt
       61 gtgcggatgt gctttcgata acccgattca gcgcctgatt atgttgcgtg ttttgatgtc
      121 gggttcttca gacggtgaag gcgagagagt tattgatcat caggtgcttg ctgatttctg
      181 ctgttgttct aagcaagcga tattcaggga aaccctggca ctggaaagag ctggttatct
      241 tcatatccga aaaattgcaa cgcttactat tgatgcaaaa gccagactac aacctgcgcg
      301 tggctacaca attctcatgc tgcggaagga ggttgtatga gccgttacgc ccccacaccg
      361 gaagttatgg ctattggtca aattaatatt t
//
LOCUS       DAZFVO020000523          380 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZFVO020000523 DAZFVO020000000
VERSION     DAZFVO020000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.533"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>380
                     /locus_tag="ACHKNB_RS27270"
                     /old_locus_tag="pgaptmp_005454"
     CDS             <1..>380
                     /locus_tag="ACHKNB_RS27270"
                     /old_locus_tag="pgaptmp_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198748.1"
                     /translation="QLLNELDEQAKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETE
                     EQQFQAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASR
                     PAERIEKVRPDNRYFTDEKSAMGS"
BASE COUNT          115 a           86 c          120 g           59 t
ORIGIN      
        1 cagttgttga atgaactgga cgaacaggcg aagcgacagg ctgtgatgaa tgagctgaag
       61 gcgaagccgc gttctgaact gcttgaggaa taccgcagac tcagccagaa agaggggcgc
      121 accgagactg aagaacaaca gtttcaggca atacgagaag tcattcgccc acaacaggaa
      181 gtgacgccgg aagcacagtc acagcctgaa aatgcggagg atggtaacgg gagcatttac
      241 ccgacggtgc ggttccggga cccgaatgaa gtccgcattg aaattaacgg gaatggtgcg
      301 tccagaccag cggaacgcat tgaaaaggtg cgcccggaca accgttattt cacggatgag
      361 aaaagcgcca tggggagtga
//
LOCUS       DAZFVO020000524          368 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZFVO020000524 DAZFVO020000000
VERSION     DAZFVO020000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.534"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>368
                     /locus_tag="ACHKNB_RS27275"
                     /old_locus_tag="pgaptmp_005455"
     CDS             <1..>368
                     /locus_tag="ACHKNB_RS27275"
                     /old_locus_tag="pgaptmp_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198749.1"
                     /translation="ISRTKAALAKNPKNRLLAVVWMQGEFDIDAKPTEHSALFLAMVE
                     KFRADLAEQAEQCTGGSAAGVPWICGDTTYFWKQKNEQAYQAIYDGYKNKTDKNIHFV
                     PLMTDENGANVPTNNPAEDP"
BASE COUNT          110 a           84 c          102 g           72 t
ORIGIN      
        1 atcagccgga caaaagcggc actggcgaag aacccgaaaa accgcctgct tgctgtggtg
       61 tggatgcagg gcgagtttga tattgatgcg aagccgacgg agcattctgc gctgtttctg
      121 gcgatggtgg aaaaattccg cgcagacctg gctgaacagg cggaacagtg taccggtggc
      181 agtgctgctg gcgttccgtg gatttgtggt gataccacgt atttctggaa acagaaaaac
      241 gaacaggcat accaggcaat ctatgacggc tacaaaaata aaacagataa aaacattcat
      301 ttcgtcccat taatgacaga tgaaaatggc gcgaatgtac ccaccaacaa tccggcagaa
      361 gacccgga
//
LOCUS       DAZFVO020000525          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZFVO020000525 DAZFVO020000000
VERSION     DAZFVO020000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.535"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>365
                     /locus_tag="ACHKNB_RS27280"
                     /old_locus_tag="pgaptmp_005456"
     CDS             <1..>365
                     /locus_tag="ACHKNB_RS27280"
                     /old_locus_tag="pgaptmp_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGU4198750.1"
                     /translation="NQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQL
                     ACDALQMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACV
                     ESFFHSLKVECIHGEHFISR"
BASE COUNT           86 a           86 c          111 g           82 t
ORIGIN      
        1 aaccagaagt gggcaggaga catcacgtac ttacgtacag atgaaggctg gctgtatctg
       61 gcagtggtca ttgacctgtg gtcacgtgcc gttattggct ggtcaatgtc gccacgcatg
      121 acggcgcaac tggcctgcga tgccctgcag atggcgctgt ggcggcgtaa gaggccccgg
      181 aacgttatcg ttcacacgga ccgtggaggc cagtactgtt cagcagatta tcaggcgcaa
      241 ctgaagcggc ataatctgcg tggaagtatg agcgcaaaag gttgctgcta cgataatgcc
      301 tgcgtggaaa gcttctttca ttcgctgaaa gtggaatgta tccatggaga acactttatc
      361 agccg
//
LOCUS       DAZFVO020000526          365 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZFVO020000526 DAZFVO020000000
VERSION     DAZFVO020000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.536"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            127..202
                     /locus_tag="ACHKNB_RS27285"
                     /old_locus_tag="pgaptmp_005457"
     tRNA            127..202
                     /locus_tag="ACHKNB_RS27285"
                     /old_locus_tag="pgaptmp_005457"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:161..163,aa:Glu,seq:ttc)
BASE COUNT          105 a           82 c           86 g           92 t
ORIGIN      
        1 gaaagtgaaa agcaaggcgt ttacgcgttg ggagtgaggc tgaagagaat aaggccgttc
       61 gctttctatt aatgaaagct caccctacac gaaaatatca cgcaacgcgt gataagcaat
      121 tttcgtgtcc ccttcgtcta gaggcccagg acaccgccct ttcacggcgg taacaggggt
      181 tcgaatcccc taggggacgc cacttgctgg tttgtgagtg aaagtcgccg accccaatat
      241 ctcaaaacag actgttaagt cttgtttgag atatttgctc tttaaaaatc tggatcaagc
      301 tgaaaattga aacactgaac aacgaaagtt gttcgtgagt ctctcaaatt ttcgcaactc
      361 tgaag
//
LOCUS       DAZFVO020000527          361 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZFVO020000527 DAZFVO020000000
VERSION     DAZFVO020000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.537"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(110..>361)
                     /locus_tag="ACHKNB_RS27290"
                     /old_locus_tag="pgaptmp_005458"
     CDS             complement(110..>361)
                     /locus_tag="ACHKNB_RS27290"
                     /old_locus_tag="pgaptmp_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4198751.1"
                     /translation="ERLPQHEDKLMTIAERLEQQGIQKGIQQGIQQGIQQGIQQGIQK
                     GRMEEKQDIARQLQKTGMPSAQICQITGLSEAELKNITH"
BASE COUNT           63 a           99 c           74 g          125 t
ORIGIN      
        1 gcaccttttt caaaaccttt ctgtataaga cgttctgcaa tcgtcatact gtttgctctg
       61 ctcctgctga caaaaaaacc cggcattttg ccgggctttg tggttctcat cagtgagtga
      121 tgttcttcag ttcagcttcg gaaagtccgg tgatctgaca gatttgtgca gatggcattc
      181 ccgttttctg aagctgacgc gcaatatcct gcttctcctc catgcggcct ttctgtattc
      241 cctgctgtat tccctgctgt attccctgct gtattccctg ctgtattcct ttctgtattc
      301 cttgctgctc aagacgttcg gcaatagtca tcagtttatc ctcatgctgc ggcagacgct
      361 c
//
LOCUS       DAZFVO020000528          356 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZFVO020000528 DAZFVO020000000
VERSION     DAZFVO020000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.538"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>356
                     /locus_tag="ACHKNB_RS27295"
                     /old_locus_tag="pgaptmp_005459"
     CDS             <1..>356
                     /locus_tag="ACHKNB_RS27295"
                     /old_locus_tag="pgaptmp_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HGU4198752.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZFVO020000529          350 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZFVO020000529 DAZFVO020000000
VERSION     DAZFVO020000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.539"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(194..334)
                     /locus_tag="ACHKNB_RS27300"
                     /old_locus_tag="pgaptmp_005460"
     CDS             complement(194..334)
                     /locus_tag="ACHKNB_RS27300"
                     /old_locus_tag="pgaptmp_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HGU4198753.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT           77 a          104 c           78 g           91 t
ORIGIN      
        1 taccgctttt cccgtaccat ccttcagcac acccgatatt ctgactgtca tggatttccc
       61 ccataaaaaa accgcagtac cggtttcccg gcctgcggta aaatttgtgg tttgttggtg
      121 ttaaaacggt gccatccggc tgaccaccct cagcaaccgg tcggcggggg atattctccc
      181 ccgccacggt ttcttactgc ttacactgta agaacgccgc aatctccgcg cccgctatcc
      241 ggaaccggaa ctcgcacagt gaactgtggg tgatccagat aatgagcact accgtgatac
      301 aaatcacggt ggtttttaac ggtttttgcg acataaacgc ttgctccttt
//
LOCUS       DAZFVO020000530          349 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZFVO020000530 DAZFVO020000000
VERSION     DAZFVO020000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.540"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>349)
                     /locus_tag="ACHKNB_RS27305"
                     /old_locus_tag="pgaptmp_005461"
     CDS             complement(<1..>349)
                     /locus_tag="ACHKNB_RS27305"
                     /old_locus_tag="pgaptmp_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGU4198754.1"
                     /translation="KNMQDNINSLMAEHGINDAMFMLMSLLSTSDNFCMSPSDISNVL
                     QFSKTNVTRISDSLVTKGFIRRVDSRNDRRSKYIHLTPEGDLFLQRVTRIKSLYLEKI
                     WSVLSDDEIKTFE"
BASE COUNT          110 a           65 c           53 g          121 t
ORIGIN      
        1 atttcgaatg tcttaatttc atcatcggat aaaactgacc agattttctc cagatataga
       61 ctttttattc tggttacccg ttgtaaaaac aaatcacctt ccggagttaa atgaatatat
      121 ttactacgac gatcattcct gctatcaact cgtcttataa atcctttggt aactaaagaa
      181 tcgctaattc tggtaacatt tgttttgctg aattgcagca cattactgat gtctgatgga
      241 gacatacaga aattatccga agtggaaagt agagacatta gcataaacat ggcatcattg
      301 attccatgtt cagccatcag actatttata ttatcctgca tatttttat
//
LOCUS       DAZFVO020000531          338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZFVO020000531 DAZFVO020000000
VERSION     DAZFVO020000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.541"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>338
                     /locus_tag="ACHKNB_RS27310"
                     /old_locus_tag="pgaptmp_005462"
     CDS             <1..>338
                     /locus_tag="ACHKNB_RS27310"
                     /old_locus_tag="pgaptmp_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198755.1"
                     /translation="LVSVINADADAPFSAYAGQVADNASGLKGSMGINARFTGECSVH
                     DIRVNYHDGETTPGGLTIELGYPKTKATNPDITRSVAGMGDRQYNYVVMPYKDDANLK
                     IISDELLKRWG"
BASE COUNT           92 a           78 c           88 g           80 t
ORIGIN      
        1 ctggtctctg ttattaatgc cgatgctgat gcgcccttca gtgcttatgc aggtcaggta
       61 gcagataacg ccagcggact caagggaagt atgggaatta atgcccgttt taccggtgaa
      121 tgttccgtac atgatatccg tgttaattat catgacggag aaaccacacc gggtggactg
      181 accattgaac ttgggtatcc caaaacaaaa gccactaacc cggatatcac ccgaagcgtg
      241 gcgggtatgg gcgatcgcca gtacaactat gtcgtcatgc cctataagga tgatgcaaac
      301 ctgaaaatca tcagtgatga gcttctgaaa cgctgggg
//
LOCUS       DAZFVO020000532          338 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZFVO020000532 DAZFVO020000000
VERSION     DAZFVO020000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.542"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(2..154)
                     /gene="hokA"
                     /locus_tag="ACHKNB_RS27315"
                     /old_locus_tag="pgaptmp_005463"
     CDS             complement(2..154)
                     /gene="hokA"
                     /locus_tag="ACHKNB_RS27315"
                     /old_locus_tag="pgaptmp_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HGU4198756.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
BASE COUNT           93 a           77 c           62 g          106 t
ORIGIN      
        1 cttactcttt taaattgcag gctaaaaatg ccgccagctc ataactcccc tgtttaatat
       61 gcaattcaca cagtgaatct cttatcatcc aggtgaaaaa taaaagcgtg aaacaaatca
      121 ctattaaaga aagtaatcta tatttctgcg gcattcccag ccccctgtgt tgatttccaa
      181 cgagtaagag gctaacctta tggtgttcag gcatagggta gcctcacttt gatttatagt
      241 caggtggggc ttttctctgt ctgcctttcg gtgaatacct gagacaaaca gtctcaagca
      301 cccgtggcta ttctagctta ataagtttgt ttcttctc
//
LOCUS       DAZFVO020000533          337 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZFVO020000533 DAZFVO020000000
VERSION     DAZFVO020000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.543"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>337
                     /locus_tag="ACHKNB_RS27320"
                     /old_locus_tag="pgaptmp_005464"
     CDS             <1..>337
                     /locus_tag="ACHKNB_RS27320"
                     /old_locus_tag="pgaptmp_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HGU4198757.1"
                     /translation="LSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVET
                     ATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQM"
BASE COUNT           71 a           96 c          118 g           52 t
ORIGIN      
        1 gctgtcacgg aaaccgcaag tgacctggta cggctgggac ggcgaccggc tgaccacgat
       61 acagaacgac aggagccgca tccagacgat ttatcagccg gggagcttca caccactcat
      121 cagagttgaa actgccaccg gtgaactggc cagaacgcag cgccgcagcc tggcggatgc
      181 gcttcagcag tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca
      241 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
      301 ctggctggca tcgtgcggcc tgaccgtgga gcagatg
//
LOCUS       DAZFVO020000534          332 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZFVO020000534 DAZFVO020000000
VERSION     DAZFVO020000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.544"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>332
                     /locus_tag="ACHKNB_RS27325"
                     /old_locus_tag="pgaptmp_005465"
     CDS             <1..>332
                     /locus_tag="ACHKNB_RS27325"
                     /old_locus_tag="pgaptmp_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4198758.1"
                     /translation="DNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGER
                     NSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKR
                     SLMESVEA"
BASE COUNT           95 a           83 c           82 g           72 t
ORIGIN      
        1 gataatatgc gagccgtcag tgtgcttaac ctaggtacta ttgatgaacc aggtcacgca
       61 gataacaccg ccactctcaa actgaaaaag acagcaccgt tctctgccct gctgtctgtt
      121 aatggcgagc gtaactccca gaagtcactg gcagaatgga ttgaagactg ggccgactac
      181 cttgtgggct ttgatgctaa tggtgacacc attcaggcaa ccaaagcggc tgcggcagtc
      241 cgtaaaatca caattgaagc aaaccagacc gctgattttg aagacaatga cttcagcggc
      301 aaacgctctc tgatggagtc tgtcgaagcg aa
//
LOCUS       DAZFVO020000535          331 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZFVO020000535 DAZFVO020000000
VERSION     DAZFVO020000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.545"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>331)
                     /locus_tag="ACHKNB_RS27330"
                     /old_locus_tag="pgaptmp_005466"
     CDS             complement(<1..>331)
                     /locus_tag="ACHKNB_RS27330"
                     /old_locus_tag="pgaptmp_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HGU4198759.1"
                     /translation="PLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKA
                     RYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLE
                     RITFYAK"
BASE COUNT          112 a           57 c           63 g           99 t
ORIGIN      
        1 tttttcgcat aaaatgtaat tctctctaaa agagttgatg caagtggtaa cggcaattgt
       61 atgtgatcaa caaaatcagg aaataataaa ttaaatcgtt gctgcattgc caatggttgt
      121 tgccttatat agggtgacaa cgttgaaaaa ccacatgtga tgatattgtg atagctagcg
      181 atgccgggtt tgtatcgtgc cttttcattt attttcaccg gtatagctga taaataatcc
      241 cttagcacag caggaacctt ataaaaaaat ccgcgttctg caaacctttc tcgataaaag
      301 tgataaccac tgaataaaca gaacaatgga a
//
LOCUS       DAZFVO020000536          329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZFVO020000536 DAZFVO020000000
VERSION     DAZFVO020000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.546"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>329
                     /locus_tag="ACHKNB_RS27335"
                     /old_locus_tag="pgaptmp_005467"
     CDS             <1..>329
                     /locus_tag="ACHKNB_RS27335"
                     /old_locus_tag="pgaptmp_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGU4198760.1"
                     /translation="QLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMV
                     ATLKIPMERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFR
                     HSYAMHML"
BASE COUNT           75 a           95 c          103 g           56 t
ORIGIN      
        1 cagcttgcga ccctgaaaca acggaccgaa aaagctgcca ggacggcggg gagaatgccc
       61 gccggtcagc agactcaccg gctggttccg ctctctgact cctggtacgt cagccagctg
      121 cagacgatgg tggcaacact gaaaataccc atggagcggc gtaaccgtcg cacaggaagg
      181 acagagaaag cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag
      241 gcggttgccg ccgctgctgc tgacggtgtg acgttctctg ttccggtcac accacatacg
      301 ttccgccatt cctatgcgat gcacatgct
//
LOCUS       DAZFVO020000537          329 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZFVO020000537 DAZFVO020000000
VERSION     DAZFVO020000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.547"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(14..238)
                     /locus_tag="ACHKNB_RS27340"
                     /old_locus_tag="pgaptmp_005468"
     CDS             complement(14..238)
                     /locus_tag="ACHKNB_RS27340"
                     /old_locus_tag="pgaptmp_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGU4198761.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
BASE COUNT           82 a          110 c           61 g           76 t
ORIGIN      
        1 tttctgatat gcatcatgtt tccgtgccct cctccgatac cggcacccag accggcattt
       61 tttccgcacc gacgccccgc ttcatacctg ccggtgcagc atccgctgca aagactttat
      121 acaccacata atccaccaca ataccgtctt ccggccagcg ccctgctgcc tcataaaccg
      181 gccgcaggga caggggataa aacatattct ctgacggtga aaaaacatac tgttccatac
      241 aatgccctca gtatccgatc gcttcccatg aaaacttgcc gtagcatccc ctgactgtta
      301 tcagagtcac agacactaat gacggattc
//
LOCUS       DAZFVO020000538          327 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZFVO020000538 DAZFVO020000000
VERSION     DAZFVO020000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.548"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>327
                     /locus_tag="ACHKNB_RS27345"
                     /old_locus_tag="pgaptmp_005469"
     CDS             <1..>327
                     /locus_tag="ACHKNB_RS27345"
                     /old_locus_tag="pgaptmp_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGU4198762.1"
                     /translation="FGNLASGAMSATAGLVTAARNEVALAEAQLRGTQIATARARAAV
                     YRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAARTAAQNALNSTTAVGSRLMSGA
                     LGLVGGV"
BASE COUNT           66 a           87 c          119 g           55 t
ORIGIN      
        1 ctttggtaat ctggcttccg gagcgatgtc tgccacggca ggacttgtga ccgctgcacg
       61 taatgaagtg gcactggctg aggcccagtt aaggggaacg cagattgcca cggcgcgggc
      121 aagggcagcc gtgtaccggg ctcagcaggc tgtggcggca gcccgcggga ctgagatgca
      181 gattgcggca gaggcccgtc tggcggtcac acaggaacgc ctgaacagaa atattgctgc
      241 cagaaccgcc gcccagaatg cgctgaacag tacaacggcg gtgggctcac gtctgatgag
      301 cggtgcgctg ggactggttg gaggcgt
//
LOCUS       DAZFVO020000539          325 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZFVO020000539 DAZFVO020000000
VERSION     DAZFVO020000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.549"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(1..325)
                     /locus_tag="ACHKNB_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /pseudo
     CDS             complement(1..325)
                     /locus_tag="ACHKNB_RS27350"
                     /old_locus_tag="pgaptmp_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           98 a           73 c           43 g          111 t
ORIGIN      
        1 tcaataaatt cttttttaaa agacaaaaaa ctccatgata aaaaaccacc ctgccctgaa
       61 ttttgttgtc agattgtaga ttaacgccaa ataacgtctt tacatcttcc ctgagattat
      121 agaatgccgt atcaacaaga gcctcgatcg tttgtttatc aacaatttta ataaactctt
      181 cgtatacttg cttttgcgtc ttatggccgc ccagcgccca tgaatactca tgttctttta
      241 aaaccagtat ggctcttaac agacaatatc catcgccatc tactggagcg tattttaatc
      301 catatgtttt attaatttca ctctg
//
LOCUS       DAZFVO020000540          324 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZFVO020000540 DAZFVO020000000
VERSION     DAZFVO020000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.550"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..302
                     /locus_tag="ACHKNB_RS27355"
                     /old_locus_tag="pgaptmp_005471"
     CDS             <1..302
                     /locus_tag="ACHKNB_RS27355"
                     /old_locus_tag="pgaptmp_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HGU4198763.1"
                     /translation="EYERHRLTRAQADAQELKNARDSAEVVETAFCTFVLSRIAGEIA
                     SILDGIPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT           80 a           80 c           93 g           71 t
ORIGIN      
        1 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggaa ctgaagaatg
       61 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
      121 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
      181 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
      241 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
      301 aacaggctgc ggcattttgt ccgc
//
LOCUS       DAZFVO020000541          323 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZFVO020000541 DAZFVO020000000
VERSION     DAZFVO020000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.551"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            100..179
                     /locus_tag="ACHKNB_RS27360"
                     /old_locus_tag="pgaptmp_005472"
     tRNA            100..179
                     /locus_tag="ACHKNB_RS27360"
                     /old_locus_tag="pgaptmp_005472"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(181..>323)
                     /locus_tag="ACHKNB_RS27365"
                     /old_locus_tag="pgaptmp_005473"
     CDS             complement(181..>323)
                     /locus_tag="ACHKNB_RS27365"
                     /old_locus_tag="pgaptmp_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HGU4198764.1"
                     /translation="VNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGD
                     ID"
BASE COUNT           87 a           88 c           73 g           75 t
ORIGIN      
        1 gcaaagaccc gctggagacg cgtaacgtca acaacgacag cggcatcaat ggatgacata
       61 ttgcctccgc ataaaggatg aatataaaat aaacaggcgg tcagtgtacg cttaactggt
      121 aaagtcggtg cctgcctccg ggtggatcat gtatgcgggt tcgacccccg ccactgaccg
      181 ttaatcaata tctccttcca gtacctcaag cattccgccc cggatatccg atttcagctt
      241 atccatattg ataacccggt aagcattaac aatcacatcc agtttatccg gctgacgctt
      301 caggctgtac ggtgcattaa cgg
//
LOCUS       DAZFVO020000542          322 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZFVO020000542 DAZFVO020000000
VERSION     DAZFVO020000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.552"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(124..322)
                     /locus_tag="ACHKNB_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /pseudo
     CDS             complement(124..322)
                     /locus_tag="ACHKNB_RS27370"
                     /old_locus_tag="pgaptmp_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          111 a           63 c           53 g           95 t
ORIGIN      
        1 aggagtattt gatcatgtat aagctatgaa aaacagcttt aggtaataag gaatatctca
       61 atttttaaac ataaaatgcg aattatttag tacaaaaaag tagcgttacg tgatctttcc
      121 ctgggataca actctggcaa tggtatcgtg aacaggaata ccattttcaa aatcaccata
      181 ttgcttcaaa aaatcgagat gtgtttcccc gaaatcctct atatcttccc aaccttctgc
      241 accagaaata acggcacaaa tagtcaacag tagaatatct gacaatttat gcaccacttt
      301 ccaggcttgt ctgtaatcgg gt
//
LOCUS       DAZFVO020000543          316 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZFVO020000543 DAZFVO020000000
VERSION     DAZFVO020000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.553"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>316
                     /locus_tag="ACHKNB_RS27375"
                     /old_locus_tag="pgaptmp_005475"
     CDS             <1..>316
                     /locus_tag="ACHKNB_RS27375"
                     /old_locus_tag="pgaptmp_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HGU4198765.1"
                     /translation="LSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAA"
BASE COUNT           79 a           81 c           77 g           79 t
ORIGIN      
        1 ctttccactg ccagcatcga tgattttacc cgttattcta aagatcttgc agatgaaggc
       61 acccgctgct ttatcgatgc cgataatatg cgtgccgtca gtgtgcttaa cctgggtact
      121 attgatgagc caggtcacgc agataacact gccactctca aactgaaaaa gacagcaccg
      181 ttctctgctc tgttgtctgt taacggcgag cgtaactccc agaagtcact ggcagaatgg
      241 atcgaagact gggccgacta ccttgtgggc tttgatgcta atggtgacgc cattcaagca
      301 acaaaagcgg ctgcgg
//
LOCUS       DAZFVO020000544          314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZFVO020000544 DAZFVO020000000
VERSION     DAZFVO020000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.554"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>314)
                     /locus_tag="ACHKNB_RS27380"
                     /old_locus_tag="pgaptmp_005476"
     CDS             complement(<1..>314)
                     /locus_tag="ACHKNB_RS27380"
                     /old_locus_tag="pgaptmp_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198766.1"
                     /translation="AIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDD
                     SHVVTMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQ
                     DKA"
BASE COUNT           78 a           95 c           79 g           62 t
ORIGIN      
        1 gccttatcct ggatgatgct ggtggcagga accgaaggca caaggtcact ttcccgggta
       61 acagacggca caacaggctt cggtaatctc agtgccttac gggctgcact ttccggtaag
      121 gcatccgcca gatcattacg aatcagccac cagcacagtt ccggcattgt cacaacgtga
      181 ctgtcatcaa aaccgagatc ccgacgcaca acagacaaca cccagcgggc acagttatcc
      241 gttgccattg attccagccg ttccgtgaac tgatcgcgca gctggttatc gcagtgccag
      301 cacagacgga ttgc
//
LOCUS       DAZFVO020000545          314 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZFVO020000545 DAZFVO020000000
VERSION     DAZFVO020000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.555"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>314)
                     /locus_tag="ACHKNB_RS27385"
                     /old_locus_tag="pgaptmp_005477"
     CDS             complement(<1..>314)
                     /locus_tag="ACHKNB_RS27385"
                     /old_locus_tag="pgaptmp_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198767.1"
                     /translation="AIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDD
                     SHVVTMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQ
                     DKA"
BASE COUNT           80 a           94 c           78 g           62 t
ORIGIN      
        1 gccttatcct ggatgatgct ggtggcagga accgaaggca caaggtcact ttcccgggtg
       61 acagacggca caacaagatt tggtaatctc agtgccttac gggctgcact ttccggtaag
      121 gcatccgcca agtcattacg aatcagccac cagcacagtt ccggcattgt cacaacgtga
      181 ctatcatcaa aaccgagatc ccgacgcaca acagacaaca cccagcgggc acagttatcc
      241 gtcgccattg attccagccg ttccgtgaac tgatcgcgca gctggttatc gcagtgccag
      301 cacagacgga ttgc
//
LOCUS       DAZFVO020000546          312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZFVO020000546 DAZFVO020000000
VERSION     DAZFVO020000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.556"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..243)
                     /gene="ydjO"
                     /locus_tag="ACHKNB_RS27390"
                     /old_locus_tag="pgaptmp_005478"
     CDS             complement(<1..243)
                     /gene="ydjO"
                     /locus_tag="ACHKNB_RS27390"
                     /old_locus_tag="pgaptmp_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HGU4198768.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTY"
BASE COUNT          102 a           61 c           50 g           99 t
ORIGIN      
        1 atacgtatca aaactaagtg ctggcgctcc tggctttgag gcatcatagt taatgttaga
       61 catataggca atcatacgat tgaagttcga ctctgctatt tcatcgcagt cacattgacc
      121 cgttgatata aagaaagtga gtgccgccat ggtagactca agcctttctt taaaagagtc
      181 gaaagccagg cttttaatat ttaaatcacc ataattactc tgtattaagt ttgtagaaaa
      241 catctcccgc cccatattgt taacaaaatt attatctcat ttaaatctaa gtcacttaca
      301 atataagttt aa
//
LOCUS       DAZFVO020000547          312 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZFVO020000547 DAZFVO020000000
VERSION     DAZFVO020000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.557"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>312
                     /locus_tag="ACHKNB_RS27395"
                     /old_locus_tag="pgaptmp_005479"
     CDS             <1..>312
                     /locus_tag="ACHKNB_RS27395"
                     /old_locus_tag="pgaptmp_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rhs family protein"
                     /protein_id="HGU4198769.1"
                     /translation="AGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDG
                     YRPETERFRWDSRDQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSI
                     AE"
BASE COUNT           83 a           87 c          108 g           34 t
ORIGIN      
        1 gcgggcaggc accggataag tgaagagact gaccagtatg ccggaggcca ccggctgaaa
       61 caggccggaa acacgcagta tgactatgac gccgcaggcc ggatggtcag ccggacaaaa
      121 caccgtgacg gctaccgccc ggaaacagag cggttccggt gggacagccg ggaccagctg
      181 accgggtatt gcagcgcaca gggggagctg tgggaatacc gccacgacgc cagcggcaga
      241 cgaacagaga aacgctgcga ccggaagaaa atccgcttca cgtacctgtg ggacggcgac
      301 agtattgcgg aa
//
LOCUS       DAZFVO020000548          304 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZFVO020000548 DAZFVO020000000
VERSION     DAZFVO020000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.558"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>304)
                     /locus_tag="ACHKNB_RS27400"
                     /old_locus_tag="pgaptmp_005480"
     CDS             complement(<1..>304)
                     /locus_tag="ACHKNB_RS27400"
                     /old_locus_tag="pgaptmp_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198770.1"
                     /translation="EYGLQQMLEGYRIILAREFFTPENRKSVPAHDESWVIICDALLG
                     RLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTMSEMVVMFGYV"
BASE COUNT           76 a          101 c           53 g           74 t
ORIGIN      
        1 tacacgtatc caaacatcac caccatctcg gacatcgtca gaggtcgcgc atacgtatta
       61 tgtacccact caccattccg gattttcctg tacgtctcca ggcgccccgt aacatcttcc
      121 gcatcaatct gaagataacg gcgtccctga aacatgcagc acatcagcct gccaaggagg
      181 gcatcacaga ttatcaccca tgactcatca tgtgccggta cactcttcct gttctctggt
      241 gtaaaaaatt ccctcgccag tattatcctg tacccctcaa gcatctgctg caggccatac
      301 tcca
//
LOCUS       DAZFVO020000549          302 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZFVO020000549 DAZFVO020000000
VERSION     DAZFVO020000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.559"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>302
                     /locus_tag="ACHKNB_RS27405"
                     /old_locus_tag="pgaptmp_005481"
     CDS             <1..>302
                     /locus_tag="ACHKNB_RS27405"
                     /old_locus_tag="pgaptmp_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HGU4198771.1"
                     /translation="NDYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQL
                     SESIKELHQHGMVSGDPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYN"
BASE COUNT          106 a           41 c           58 g           97 t
ORIGIN      
        1 ttaatgacta ttttttgcta gctgaacgta aaacattaaa tttcgctcat tattatatta
       61 tgctcattga gtatattgaa ggtgtcgggt taaatgagta tttggaaatt tctgaagatc
      121 tgaaggatca attatcagaa tcgataaaag agttgcatca gcatggaatg gtgtctggcg
      181 atcctcataa aggaaatttt atagtatccg aaaaaggttt aagactaatt gacctttcag
      241 gtaaaaaaac aaccgctgtt ttaaaagcaa aagatcgcat tgatcttgaa cggcactata
      301 at
//
LOCUS       DAZFVO020000550          301 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZFVO020000550 DAZFVO020000000
VERSION     DAZFVO020000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.560"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>301
                     /locus_tag="ACHKNB_RS27410"
                     /old_locus_tag="pgaptmp_005482"
     CDS             <1..>301
                     /locus_tag="ACHKNB_RS27410"
                     /old_locus_tag="pgaptmp_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGU4198772.1"
                     /translation="PARLQRQLSRRVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVT
                     TTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQ"
BASE COUNT           94 a           77 c           79 g           51 t
ORIGIN      
        1 gccggcgaga cttcagcgac agttaagccg cagggtcaaa ttcagcaaca actggcagaa
       61 gcagaaacgc aaaatacagc gactgcattc ctgtatcgca aatatccgca gggactacct
      121 tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa
      181 ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc cagccggggc gcaatgtccg
      241 ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgccgcca
      301 g
//
LOCUS       DAZFVO020000551          299 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZFVO020000551 DAZFVO020000000
VERSION     DAZFVO020000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.561"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            173..>299
                     /locus_tag="ACHKNB_RS27415"
                     /old_locus_tag="pgaptmp_005483"
     CDS             173..>299
                     /locus_tag="ACHKNB_RS27415"
                     /old_locus_tag="pgaptmp_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGU4198773.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRY"
BASE COUNT           85 a           63 c           54 g           97 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tccatgagaa aacttaaaga aattcttagg cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatg
//
LOCUS       DAZFVO020000552          298 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZFVO020000552 DAZFVO020000000
VERSION     DAZFVO020000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.562"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>298)
                     /locus_tag="ACHKNB_RS27420"
                     /old_locus_tag="pgaptmp_005484"
     CDS             complement(<1..>298)
                     /locus_tag="ACHKNB_RS27420"
                     /old_locus_tag="pgaptmp_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGU4198774.1"
                     /translation="ALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLT
                     DNIDTSTPMGRFFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQE"
BASE COUNT           61 a           87 c           74 g           76 t
ORIGIN      
        1 gttcctgcgt gagacggcgg ggtctgccgc cggttcgtcc cctttcacgg gcagctgcaa
       61 gaccagcccg tgtccgttcg acaattaatt cacgctccat ttctgccagc gcacccatta
      121 tgtgaaaaaa gaagcgcccc atgggggttg acgtatcaat gttgtcagta agactacgaa
      181 aatttatccc ctgttgctgt aattcatctg tcagcgcaac aaggtggcgc atactgcgcc
      241 ccagcctgtc cagtttccag accatcagcg tgtcaccggg ttttagtttc ttcagtgc
//
LOCUS       DAZFVO020000553          296 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZFVO020000553 DAZFVO020000000
VERSION     DAZFVO020000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.563"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>296)
                     /locus_tag="ACHKNB_RS27425"
                     /old_locus_tag="pgaptmp_005485"
     CDS             complement(<1..>296)
                     /locus_tag="ACHKNB_RS27425"
                     /old_locus_tag="pgaptmp_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4198775.1"
                     /translation="LVPNQFALGIYWQLSSLPEGVTLSEEGNDMLMSFFLQTDATNLN
                     AMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPA"
BASE COUNT           65 a           90 c           71 g           70 t
ORIGIN      
        1 gccggagatt tcaccagtgt gaacggtgta ccccgtttct catccagtac cggcgtcacc
       61 tgaatgctgt tgtttccggc aaactcaaaa gccagtgtgt gccatccgtt atcaaagacc
      121 ccgaacgtat ccagcttcgc attctgcttc ttgtggtaca tcgcgttcag gttcgtcgca
      181 tccgtctgca ggaagaagga catcagcatg tcgttgcctt cctctgacag cgtcactccc
      241 tccggcaggg acgacaactg ccagtaaata cccagggcaa actgattcgg caccag
//
LOCUS       DAZFVO020000554          293 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZFVO020000554 DAZFVO020000000
VERSION     DAZFVO020000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.564"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            1..>293
                     /locus_tag="ACHKNB_RS27430"
                     /old_locus_tag="pgaptmp_005486"
     rRNA            1..>293
                     /locus_tag="ACHKNB_RS27430"
                     /old_locus_tag="pgaptmp_005486"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           86 a           64 c           86 g           57 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg tta
//
LOCUS       DAZFVO020000555          292 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZFVO020000555 DAZFVO020000000
VERSION     DAZFVO020000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.565"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>292
                     /locus_tag="ACHKNB_RS27435"
                     /old_locus_tag="pgaptmp_005487"
     CDS             <1..>292
                     /locus_tag="ACHKNB_RS27435"
                     /old_locus_tag="pgaptmp_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HGU4198776.1"
                     /translation="ESGDRIIRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLG
                     ILRDEYAAMPETVSDGITKVENAFMAWVGGANEASGATKTLSGALNGVAGNI"
BASE COUNT           68 a           66 c          106 g           52 t
ORIGIN      
        1 atgaaagtgg ggaccggatc attcgcgcac tggctgcagg catgggcgtg gcccgtaagg
       61 atctgaaggc gatggcggac gatggtcaac tgacggcgga taaagtcgtt cccgcgttaa
      121 tcagccagct ggggatattg cgtgatgaat atgcagccat gccggaaacg gtgtctgacg
      181 ggatcacgaa ggtggaaaac gcctttatgg cctgggtggg cggcgcgaat gaggccagcg
      241 gagcgacgaa aacgctctcc ggcgcactga acggtgtggc cggaaatatt ga
//
LOCUS       DAZFVO020000556          291 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZFVO020000556 DAZFVO020000000
VERSION     DAZFVO020000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.566"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..249)
                     /locus_tag="ACHKNB_RS27440"
                     /old_locus_tag="pgaptmp_005488"
     CDS             complement(<1..249)
                     /locus_tag="ACHKNB_RS27440"
                     /old_locus_tag="pgaptmp_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HGU4198777.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANH"
BASE COUNT           92 a           55 c           55 g           89 t
ORIGIN      
        1 atgatttgca tcaccggatt taatctcaac ttcattttcc tgaggcgtcg atggattatc
       61 gatacggaat actgtcgcct tgatgtactg aacttcatta cttttagata ttacgcgaac
      121 gttgccttca ccctgactat taatggaaac agccatagga taaacagtca tatttgactg
      181 tgccaaaaaa ggagcgaaca cgaataaaag cggcgtaagc cgtgataaga gattaatttt
      241 cgccaccata taaatttgat cttacaatat tgtttctaaa aagttggtgt a
//
LOCUS       DAZFVO020000557          286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZFVO020000557 DAZFVO020000000
VERSION     DAZFVO020000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.567"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(2..>286)
                     /locus_tag="ACHKNB_RS27445"
                     /old_locus_tag="pgaptmp_005489"
     CDS             complement(2..>286)
                     /locus_tag="ACHKNB_RS27445"
                     /old_locus_tag="pgaptmp_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HGU4198778.1"
                     /translation="AELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAE
                     LRNNEIFSRWDRGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
BASE COUNT           60 a           84 c           77 g           65 t
ORIGIN      
        1 atcagaaaag tggcggttgc gttcttgcca gatgcagacg gcgctgttcg cgtatgacgg
       61 tataaatctg ggtttctgac atccggtaat ggcggcgaag ggattcaatt ttctcgcccc
      121 ggtcccagcg cgaaaatatc tcattgtttc gcagctcggc aaagagagat tccccgaccg
      181 gcaggtaata accccgtccg cccatatagg ctgcctgagc cgcagccacc ttgcgtgcca
      241 gtttaccggc cagtaccggc tcaatcccct gacgctgaag ttctgc
//
LOCUS       DAZFVO020000558          286 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZFVO020000558 DAZFVO020000000
VERSION     DAZFVO020000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.568"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>286
                     /locus_tag="ACHKNB_RS27450"
                     /old_locus_tag="pgaptmp_005490"
     CDS             <1..>286
                     /locus_tag="ACHKNB_RS27450"
                     /old_locus_tag="pgaptmp_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198779.1"
                     /translation="GSRTLFPRLEPVHVDSAMAWQLLGFPDVWVRHEELDDVLKKQQQ
                     NEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLSKLTSARLATLCEA"
BASE COUNT           65 a           61 c           97 g           63 t
ORIGIN      
        1 ccggaagccg cacattgttc ccacgtcttg agccggtgca tgttgacagt gcgatggcct
       61 ggcaactgct ggggtttccg gatgtctggg ttcgtcatga agagcttgat gatgttctga
      121 aaaagcaaca acagaatgag cagttgcggc aggcacagca ggcgcaggaa agagtgcttg
      181 ctgcgcgggc agaagcggag aacagttttg ttgtttctgt taacgggcag gaggtggatt
      241 taagtaagct cacctcagca cggctggcga cgctgtgtga ggcaga
//
LOCUS       DAZFVO020000559          284 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZFVO020000559 DAZFVO020000000
VERSION     DAZFVO020000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.569"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHKNB_RS27455"
                     /old_locus_tag="pgaptmp_005491"
     CDS             complement(<1..234)
                     /locus_tag="ACHKNB_RS27455"
                     /old_locus_tag="pgaptmp_005491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198780.1"
                     /translation="MKHLESSGSALYFSRPGGEFHTLTAGSMDISDSVLVMRTDLNHS
                     DQLRVTESLRGKNNLLLVDFTERSDGQKALNIPL"
BASE COUNT           68 a           89 c           62 g           65 t
ORIGIN      
        1 cagagggata ttcagcgcct tctgcccgtc agaacgctca gtaaaatcaa ccaacaacag
       61 gttattctta ccccgcagcg attctgtcac ccgaagctgg tctgagtggt taaggtccgt
      121 ccgcatcacc agaacactgt cgctgatatc catactgcct gccgtcaggg tatggaactc
      181 cccgcccggc ctgctgaaat acagagcact tcccgatgac tcaagatgct tcacggatga
      241 gtttcccgtc atcatccagg ccgcattctg cagattcaca ttac
//
LOCUS       DAZFVO020000560          280 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZFVO020000560 DAZFVO020000000
VERSION     DAZFVO020000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.570"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           74 a           51 c           59 g           96 t
ORIGIN      
        1 ctcctcctca tcagttcgta gcagagtgta ctcagcatgg caagttcagc ttctgatgat
       61 ttacagtgtg tattcaggag ggtaagaaat tgttcaaaaa catcagggtt tatactggtg
      121 ctttgatatt cgttactcat agtatttcct ttcaggtcat tattacttcc ggatttaaca
      181 tgatgtctgt aaccataaaa taatgaacgt gttccgggag tccttgccgt gtagtttccc
      241 gtaaaaatac tgttgtgcat tacatgtcat taggtggaaa
//
LOCUS       DAZFVO020000561          279 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZFVO020000561 DAZFVO020000000
VERSION     DAZFVO020000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.571"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..161
                     /locus_tag="ACHKNB_RS27460"
                     /old_locus_tag="pgaptmp_005492"
     CDS             <1..161
                     /locus_tag="ACHKNB_RS27460"
                     /old_locus_tag="pgaptmp_005492"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HGU4198781.1"
                     /translation="FAEYAASLEKSGYYAEAIDEWEKAYSYARGENVSWCQARINRCK
                     KAGRLFTR"
BASE COUNT          106 a           42 c           55 g           76 t
ORIGIN      
        1 cctttgctga gtatgcagca agccttgaaa aatcaggtta ttacgccgaa gctattgatg
       61 aatgggaaaa agcttattcg tatgcccgtg gtgagaatgt ttcctggtgc caggcaagaa
      121 taaaccgttg taaaaaagca ggaagattat ttaccagata atacgaaaaa aacaatatta
      181 ttatgttatc cttaaagtaa gtgaggtcat gaaatggaca gcatcaaatt tcttgatgaa
      241 ataatagcat atcaacaaca taaagatgat aaatatcct
//
LOCUS       DAZFVO020000562          277 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZFVO020000562 DAZFVO020000000
VERSION     DAZFVO020000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.572"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>277)
                     /locus_tag="ACHKNB_RS27465"
                     /old_locus_tag="pgaptmp_005493"
     CDS             complement(<1..>277)
                     /locus_tag="ACHKNB_RS27465"
                     /old_locus_tag="pgaptmp_005493"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGU4198782.1"
                     /translation="AGEKALVKLPKSKTNISFKSKNCVSFTLFFRREVINAVGRFDQN
                     MGVGAGTNYGAGEESDFVVRALSLGFKGLYYPDYYVLHPAKESHPVFD"
BASE COUNT           84 a           64 c           44 g           85 t
ORIGIN      
        1 catcaaatac tggatggctt tctttcgctg gatgtaatac ataatagtca ggatagtaca
       61 gacctttaaa acctaaagaa agggcccgaa ctacaaagtc gctttcttct cctgcaccat
      121 agttagtgcc agctccgacc cccatatttt gatcaaatct accaacagca tttataactt
      181 cgcgcctgaa aaataatgta aatgaaacac aatttttact cttgaatgaa atattagtct
      241 tgcttttagg tagcttgacc agtgcttttt cccctgc
//
LOCUS       DAZFVO020000563          272 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZFVO020000563 DAZFVO020000000
VERSION     DAZFVO020000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.573"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>272)
                     /locus_tag="ACHKNB_RS27470"
                     /old_locus_tag="pgaptmp_005494"
     CDS             complement(<1..>272)
                     /locus_tag="ACHKNB_RS27470"
                     /old_locus_tag="pgaptmp_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198783.1"
                     /translation="STPSPDRDGDILEPEGAEFGSAIPFLWQHDHSRPVGQCTVRRVS
                     EGLEITATLVKPVPDMPSQLAARLDEVWAAIKTGLVRGLSVGFRPH"
BASE COUNT           52 a           99 c           78 g           43 t
ORIGIN      
        1 tcatggggac ggaagcccac ggacagcccc ctgaccagcc cggtcttaat ggccgcccag
       61 acctcatcca gccgggcagc cagttgcgac ggcatatccg gtacgggctt caccagtgtt
      121 gccgtgattt ccagcccttc gctgacccgg cgcaccgtac actgccccac cgggcgggaa
      181 tggtcatgct gccagagaaa cgggatcgca ctgccaaact ccgcgccctc cggctccagg
      241 atgtcaccat cccgatccgg agaaggcgtt ga
//
LOCUS       DAZFVO020000564          268 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZFVO020000564 DAZFVO020000000
VERSION     DAZFVO020000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.574"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>268)
                     /locus_tag="ACHKNB_RS27475"
                     /old_locus_tag="pgaptmp_005495"
     CDS             complement(<1..>268)
                     /locus_tag="ACHKNB_RS27475"
                     /old_locus_tag="pgaptmp_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGU4198784.1"
                     /translation="PLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGS
                     LNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGV"
BASE COUNT           99 a           61 c           36 g           72 t
ORIGIN      
        1 tacaccagag ttaaaatagc taccgttaaa atcctcatta cacaaacgct cagcgctttt
       61 actttgcata gcatctacat caattaccac tgccccatat tcatctttaa actctaatgc
      121 tattaactcg ttcaatgaac ccttacagac gatatcagca tcaagatata ataatgaatc
      181 caatcgcgca gaaaaataat caaatgaaag caaacgataa taaatagata ctggccagat
      241 atttgattga ggtagcgcct tcaatggc
//
LOCUS       DAZFVO020000565          266 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZFVO020000565 DAZFVO020000000
VERSION     DAZFVO020000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.575"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>266
                     /locus_tag="ACHKNB_RS27480"
                     /old_locus_tag="pgaptmp_005496"
     CDS             <1..>266
                     /locus_tag="ACHKNB_RS27480"
                     /old_locus_tag="pgaptmp_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198785.1"
                     /translation="LFRPGSELMPLFMQGRVLLEPEPERYSSFASGAVPAASQPLADD
                     PAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSENMT"
BASE COUNT           45 a           70 c           86 g           65 t
ORIGIN      
        1 ttgttcaggc caggttcaga gctgatgccc ctgtttatgc aggggcgtgt cctgctggag
       61 cctgagccgg agcgttattc atctttcgcc agtggtgccg ttccggcggc atcacaaccg
      121 ctggcggatg atcctgccgt tcgggccgtg ttccgcaatg aggcagtgat ccgtcgtgct
      181 ggtggcgtgg aatgtcttga aagctggtta cttcgtgaaa aaggctgcca gtggcctcat
      241 tccgactggc acagcgagaa catgac
//
LOCUS       DAZFVO020000566          265 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZFVO020000566 DAZFVO020000000
VERSION     DAZFVO020000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.576"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>265)
                     /locus_tag="ACHKNB_RS27485"
                     /old_locus_tag="pgaptmp_005497"
     CDS             complement(<1..>265)
                     /locus_tag="ACHKNB_RS27485"
                     /old_locus_tag="pgaptmp_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HGU4198786.1"
                     /translation="VTLTGDAGNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFG
                     AFDNEWHTLAFRFAGNNSLQVIPVIDGQDGAAFTLTQSPVGTFP"
BASE COUNT           64 a           66 c           61 g           74 t
ORIGIN      
        1 cgggaaagtt cctactggtg actgcgtcag ggtaaacgcg gcaccatcct gaccatcaat
       61 aaccggaata acctgaaggc tgttatttcc tgcaaaacgg aaggccagcg tatgccattc
      121 attatcaaat gcaccaaagg ttcccagttt ctggttattt gttgccactt ttgcattgtg
      181 gtacatcaca ttcaggtctt ttgcgtctgt ctggatgtag aacgatgcca gcagattatt
      241 ccctgcatcc cctgtcaggg tgaca
//
LOCUS       DAZFVO020000567          263 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZFVO020000567 DAZFVO020000000
VERSION     DAZFVO020000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.577"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            162..>263
                     /locus_tag="ACHKNB_RS27490"
                     /old_locus_tag="pgaptmp_005498"
     CDS             162..>263
                     /locus_tag="ACHKNB_RS27490"
                     /old_locus_tag="pgaptmp_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HGU4198787.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAG"
BASE COUNT           65 a           76 c           57 g           65 t
ORIGIN      
        1 aaatgcatta ccttaccccc ttcaggcgcg tgtcgtgatt ttctaacctg aatttaacgg
       61 gctttaaaat ctgcgtgaag cgcctttgta tttttcccct gaaagcactt cctgaaatcc
      121 cgcaaaaccc gccctgaaaa ttcccggcct atgctgccgg aatgaagacg aaaaacacgc
      181 ccaaacttgc ttatgccatt ctgaatgcca tcagcctgtc cgcagacggg gacggtgact
      241 ggtgtcagat catgccagcc ggt
//
LOCUS       DAZFVO020000568          258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZFVO020000568 DAZFVO020000000
VERSION     DAZFVO020000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.578"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           90 a           38 c           52 g           78 t
ORIGIN      
        1 aacccattca caaaaaatat agtattcact ctgttgattg gcaaagaaga gcgaaagctt
       61 cggataaaat attccttttg cacgtttacg cagaatgggg tagtgaaggt aactccaaat
      121 gttagagtaa ctgagaagtt tctctacatt gaatatgaaa atggcaattt atgtgtaatg
      181 tcagttcatg acttcgtaaa cggacattat attgatttag gaattcatac agaagtgcat
      241 tacgtaggat atacacgt
//
LOCUS       DAZFVO020000569          258 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZFVO020000569 DAZFVO020000000
VERSION     DAZFVO020000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.579"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>258)
                     /locus_tag="ACHKNB_RS27495"
                     /old_locus_tag="pgaptmp_005499"
     CDS             complement(<1..>258)
                     /locus_tag="ACHKNB_RS27495"
                     /old_locus_tag="pgaptmp_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HGU4198788.1"
                     /translation="QSFQSEIQRASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNS
                     QLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQA"
BASE COUNT           49 a           88 c           73 g           48 t
ORIGIN      
        1 cgcctgtttc agacgggcat tcacggagct ccattcatcc gccagcgaaa tcaggtgtcc
       61 ggtggcaaag gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt
      121 caactggctg ttcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg
      181 acgtccgcca ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat
      241 ctccgactgg aatgactg
//
LOCUS       DAZFVO020000570          257 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZFVO020000570 DAZFVO020000000
VERSION     DAZFVO020000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.580"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>257
                     /locus_tag="ACHKNB_RS27500"
                     /old_locus_tag="pgaptmp_005500"
     CDS             <1..>257
                     /locus_tag="ACHKNB_RS27500"
                     /old_locus_tag="pgaptmp_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HGU4198789.1"
                     /translation="LAWDKRPSSVLAALCLGLSHSTERVAWTGKQLLAERFPDSSRLL
                     LEDWERYLGLPECDMAGATITERQRYAGNKYRMKPSLNREF"
BASE COUNT           56 a           72 c           71 g           58 t
ORIGIN      
        1 ctggcctggg acaagcggcc gtcatccgta ctggctgcgt tatgccttgg cctcagccat
       61 tccaccgaac gtgttgcatg gacgggaaaa caactgctgg cagaacgttt ccctgacagt
      121 tcccgtctgc ttctggaaga ctgggagcgt tatctcggcc ttcccgagtg tgatatggcc
      181 ggagccacca tcacagaacg acagcgttat gccgggaata aataccggat gaagccttcg
      241 ctgaaccgtg aatttta
//
LOCUS       DAZFVO020000571          254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZFVO020000571 DAZFVO020000000
VERSION     DAZFVO020000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.582"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>254
                     /locus_tag="ACHKNB_RS27505"
                     /old_locus_tag="pgaptmp_005501"
     CDS             <1..>254
                     /locus_tag="ACHKNB_RS27505"
                     /old_locus_tag="pgaptmp_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4198790.1"
                     /translation="VANIPQPADGKSITPDDVRPMLEQMVKEAVSHIPVPRDGRDYDP
                     DVLQKAVNDAVSALPAPQDGRDATALEILPAIDDQKSFPR"
BASE COUNT           56 a           66 c           75 g           57 t
ORIGIN      
        1 cagtcgcaaa tattccgcag cctgcagacg gtaaaagtat caccccggat gatgtgcgtc
       61 cgatgcttga gcagatggtg aaggaggctg taagccatat tcctgttccg cgtgatggtc
      121 gtgactatga tccggatgtt ctgcagaagg cggtgaatga tgcggtgagt gccctgccgg
      181 ctccgcagga cgggcgtgat gccactgcac tggaaatact ccctgccatt gacgatcaaa
      241 aatcctttcc ccgg
//
LOCUS       DAZFVO020000572          254 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZFVO020000572 DAZFVO020000000
VERSION     DAZFVO020000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.583"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>254)
                     /locus_tag="ACHKNB_RS27510"
                     /old_locus_tag="pgaptmp_005502"
     CDS             complement(<1..>254)
                     /locus_tag="ACHKNB_RS27510"
                     /old_locus_tag="pgaptmp_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198791.1"
                     /translation="VVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSG
                     RSLRFACSPGEVSVLYAVAPKAGRSQIPDELLTWPEEVAA"
BASE COUNT           64 a           72 c           62 g           56 t
ORIGIN      
        1 cgcagccact tcttcaggcc atgtgaggag ttcatccggt atctggctgc gtccggcttt
       61 cggagcgacg gcataaagca cgctcacctc accgggagaa caggcaaatc gcagggagcg
      121 tccggagctg atatccacat cccgaccgac aaaaagctca tgattatcgt cagagatacg
      181 gatgatatga acgcactcct catcatcttt gtcatacgga agcacgattt cttttcctgc
      241 tactggtaca acag
//
LOCUS       DAZFVO020000573          251 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZFVO020000573 DAZFVO020000000
VERSION     DAZFVO020000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.584"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>251
                     /locus_tag="ACHKNB_RS27515"
                     /old_locus_tag="pgaptmp_005503"
     CDS             <1..>251
                     /locus_tag="ACHKNB_RS27515"
                     /old_locus_tag="pgaptmp_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HGU4198792.1"
                     /translation="TLDRHLHEPGVAFTSTRAAREWLILNMAGLEREEFRVLYLNNQN
                     QLIAGETLFTGTINRTEVHPREVIKRALYHNAAAVVLAH"
BASE COUNT           58 a           80 c           67 g           46 t
ORIGIN      
        1 accctggacc gccatcttca tgaacccggc gtggccttca cctccacccg agcggcacgg
       61 gaatggctga ttctgaacat ggcgggactg gagcgtgaag aattccgggt gctgtatctg
      121 aacaaccaga atcagctgat tgccggtgaa accctcttta ccggcaccat caaccgcacg
      181 gaagtccatc cccgggaagt gattaaacgc gccctgtacc acaatgccgc tgccgtggtg
      241 ctggcacaca a
//
LOCUS       DAZFVO020000574          250 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZFVO020000574 DAZFVO020000000
VERSION     DAZFVO020000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.585"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..117
                     /locus_tag="ACHKNB_RS27520"
                     /old_locus_tag="pgaptmp_005504"
     CDS             <1..117
                     /locus_tag="ACHKNB_RS27520"
                     /old_locus_tag="pgaptmp_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGU4198793.1"
                     /translation="WGGSLWHCNSMTGDKPGEAHSSAWTLAAKRGRDAGGGK"
     gene            114..>250
                     /locus_tag="ACHKNB_RS27525"
                     /old_locus_tag="pgaptmp_005505"
     CDS             114..>250
                     /locus_tag="ACHKNB_RS27525"
                     /old_locus_tag="pgaptmp_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HGU4198794.1"
                     /translation="MTVLLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     D"
BASE COUNT           60 a           60 c           83 g           47 t
ORIGIN      
        1 tgggggggct cgctgtggca ctgcaacagt atgaccggtg ataaacccgg agaagctcat
       61 tcatcagcct ggaccctggc tgcaaaacgt gggcgggatg ctggaggcgg aaaatgacgg
      121 tattactgac actggaagag atcaaggcac atctgcgtgt cgaccatgac gcggatgatg
      181 acatgctgat ggacaaggtt cgtcaggcta ccgccgtgct gctggcctac attcagggca
      241 gccgggataa
//
LOCUS       DAZFVO020000575          249 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZFVO020000575 DAZFVO020000000
VERSION     DAZFVO020000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.586"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..248)
                     /locus_tag="ACHKNB_RS27530"
                     /old_locus_tag="pgaptmp_005506"
     CDS             complement(<1..248)
                     /locus_tag="ACHKNB_RS27530"
                     /old_locus_tag="pgaptmp_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HGU4198795.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQA"
BASE COUNT           59 a           67 c           62 g           61 t
ORIGIN      
        1 gcctgattca gataaaaatt acccgacatg gaaatctcgg acgacgcccc cagaccgttg
       61 atgttctcct gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgaccggcg
      121 gtgaactgga cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt
      181 tcagcagtcg ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa
      241 gcagacatt
//
LOCUS       DAZFVO020000576          247 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZFVO020000576 DAZFVO020000000
VERSION     DAZFVO020000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.588"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>247
                     /locus_tag="ACHKNB_RS27535"
                     /old_locus_tag="pgaptmp_005507"
     CDS             <1..>247
                     /locus_tag="ACHKNB_RS27535"
                     /old_locus_tag="pgaptmp_005507"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HGU4198796.1"
                     /translation="QMRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAA
                     KANFAVAVGQYSQANAEKSISLGASARAIENNSTALGA"
BASE COUNT           76 a           45 c           48 g           78 t
ORIGIN      
        1 caaatgcgta aaatcagtgt cagttatata tgcaaatcat tatttctcta ctgctcagct
       61 tctttacctg tcttatctgc aaatggtatt cctgataaaa tgatcgtgga acttggtgat
      121 aacagtgcgg caaaagctaa tttcgctgtt gctgttggtc aatattcaca ggcaaatgca
      181 gaaaaaagta tttctttagg tgcatcagca agagctattg aaaataactc taccgcgtta
      241 ggtgcaa
//
LOCUS       DAZFVO020000577          246 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZFVO020000577 DAZFVO020000000
VERSION     DAZFVO020000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.589"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>246
                     /locus_tag="ACHKNB_RS27540"
                     /old_locus_tag="pgaptmp_005508"
     CDS             <1..>246
                     /locus_tag="ACHKNB_RS27540"
                     /old_locus_tag="pgaptmp_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HGU4198797.1"
                     /translation="ASAFTTGADGTYSESAGASENSLRWGVGKPLYQDLVSRTKAALA
                     KNPKNRLLAVVWMQGEGDAAVGTHAQHPGLFSAMVNQ"
BASE COUNT           55 a           54 c           89 g           48 t
ORIGIN      
        1 gtgcttcggc attcacaacg ggtgcagacg gcacatacag cgaatcagcc ggtgcatcgg
       61 aaaattcact gcgctggggt gtgggtaagc cgctgtatca ggatttggtg agccggacta
      121 aagccgcact ggcgaagaac ccgaaaaacc gtctgcttgc ggtggtgtgg atgcagggag
      181 aaggtgatgc ggcagtgggg acgcatgcgc agcatccggg gctgtttagt gcgatggtga
      241 atcagt
//
LOCUS       DAZFVO020000578          244 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZFVO020000578 DAZFVO020000000
VERSION     DAZFVO020000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.590"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..>244
                     /locus_tag="ACHKNB_RS27545"
                     /old_locus_tag="pgaptmp_005509"
     CDS             <1..>244
                     /locus_tag="ACHKNB_RS27545"
                     /old_locus_tag="pgaptmp_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HGU4198798.1"
                     /translation="PMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVR
                     ELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT           55 a           60 c           74 g           55 t
ORIGIN      
        1 gcccgatgcc gcagactcgt gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca
       61 tcatcgtgtt cctgaacaaa tgcgacatgg ttgatgacga agagctgctg gaactggttg
      121 aaatggaagt tcgtgaactt ctgtctcagt acgacttccc gggcgacgac actccgatcg
      181 ttcgtggttc tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg
      241 aact
//
LOCUS       DAZFVO020000579          236 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZFVO020000579 DAZFVO020000000
VERSION     DAZFVO020000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.591"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>236)
                     /locus_tag="ACHKNB_RS27550"
                     /old_locus_tag="pgaptmp_005510"
     CDS             complement(<1..>236)
                     /locus_tag="ACHKNB_RS27550"
                     /old_locus_tag="pgaptmp_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198799.1"
                     /translation="CTIRTIKSYDRPFSAASGNRKPVVKIASSAGAAAQSTTVFHKEK
                     TIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGR"
BASE COUNT           46 a           61 c           63 g           66 t
ORIGIN      
        1 cgcccttcct ctgcggcttt ggtcatgacc tcctcaaggg aggctgccag cgctgcacgc
       61 ttgttttcaa aacttttaat ctgttcgcca atattcatta tggtcttttc cttatgaaaa
      121 acggttgttg actgtgccgc agcgccggca gaagatgcga ttttcaccac cggtttccgg
      181 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgca
//
LOCUS       DAZFVO020000580          232 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZFVO020000580 DAZFVO020000000
VERSION     DAZFVO020000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.592"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            complement(<1..>232)
                     /locus_tag="ACHKNB_RS27555"
                     /old_locus_tag="pgaptmp_005511"
     CDS             complement(<1..>232)
                     /locus_tag="ACHKNB_RS27555"
                     /old_locus_tag="pgaptmp_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198800.1"
                     /translation="TGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPL"
BASE COUNT           39 a           75 c           77 g           41 t
ORIGIN      
        1 agcggcgcgg caggtaaatt ctccgggtat tccgggtcgt gcgtcagcca cacggcagac
       61 agacggatgc cgttgtcccg gccatattcg gtgtaacccg gcagggtatc aggaaaagcg
      121 gacggttccg tcccgccgga aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc
      181 gtggtcagta ccagccggaa gtgacgcccg gcaccatccg tcacgccggt ga
//
LOCUS       DAZFVO020000581          231 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZFVO020000581 DAZFVO020000000
VERSION     DAZFVO020000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.denovo.593"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
BASE COUNT           59 a           51 c           47 g           74 t
ORIGIN      
        1 aaaaagagta agttaacatc attgttaatt aattcgacca gtcagtaaat atgctgactg
       61 gtaggacata gtttgctaaa aagtcatcac tgactgatgc cagtcacacc atccgccagt
      121 tcaatcttcg ggtatttcca gcgtatctcg gcacgcgatt gctggtctgt tgatgtagtg
      181 gattgttgtt gctgactttt cttaccatca gatttctgcc ccctgtcaac t
//
LOCUS       DAZFVO020000582        19125 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.095, whole genome shotgun sequence.
ACCESSION   DAZFVO020000582 DAZFVO020000000
VERSION     DAZFVO020000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 19125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.095"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            <1..238
                     /locus_tag="ACHKNB_RS27560"
                     /old_locus_tag="pgaptmp_005512"
     CDS             <1..238
                     /locus_tag="ACHKNB_RS27560"
                     /old_locus_tag="pgaptmp_005512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGU4198801.1"
                     /translation="LGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQ
                     KSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKNB_RS27565"
                     /old_locus_tag="pgaptmp_005513"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHKNB_RS27565"
                     /old_locus_tag="pgaptmp_005513"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HGU4198802.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKNB_RS27570"
                     /old_locus_tag="pgaptmp_005514"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHKNB_RS27570"
                     /old_locus_tag="pgaptmp_005514"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HGU4198803.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHKNB_RS27575"
                     /old_locus_tag="pgaptmp_005515"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHKNB_RS27575"
                     /old_locus_tag="pgaptmp_005515"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HGU4198804.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMANLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKNB_RS27580"
                     /old_locus_tag="pgaptmp_005516"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHKNB_RS27580"
                     /old_locus_tag="pgaptmp_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HGU4198805.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6384..6698)
                     /locus_tag="ACHKNB_RS27585"
                     /old_locus_tag="pgaptmp_005517"
     CDS             complement(6384..6698)
                     /locus_tag="ACHKNB_RS27585"
                     /old_locus_tag="pgaptmp_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HGU4198806.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDIRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHKNB_RS27590"
                     /old_locus_tag="pgaptmp_005518"
     CDS             complement(6695..7009)
                     /gene="yjeN"
                     /locus_tag="ACHKNB_RS27590"
                     /old_locus_tag="pgaptmp_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HGU4198807.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLLDIYCAYLIRCLVFRGELVGYGDLFHMRYSEIKRPV
                     TL"
     gene            complement(7061..8563)
                     /gene="yjeM"
                     /locus_tag="ACHKNB_RS27595"
                     /old_locus_tag="pgaptmp_005519"
     CDS             complement(7061..8563)
                     /gene="yjeM"
                     /locus_tag="ACHKNB_RS27595"
                     /old_locus_tag="pgaptmp_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HGU4198808.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8782..9759)
                     /gene="epmA"
                     /locus_tag="ACHKNB_RS27600"
                     /old_locus_tag="pgaptmp_005520"
     CDS             complement(8782..9759)
                     /gene="epmA"
                     /locus_tag="ACHKNB_RS27600"
                     /old_locus_tag="pgaptmp_005520"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HGU4198809.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10084..11892
                     /gene="frdA"
                     /locus_tag="ACHKNB_RS27605"
                     /old_locus_tag="pgaptmp_005521"
     CDS             10084..11892
                     /gene="frdA"
                     /locus_tag="ACHKNB_RS27605"
                     /old_locus_tag="pgaptmp_005521"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HGU4198810.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11885..12619
                     /gene="frdB"
                     /locus_tag="ACHKNB_RS27610"
                     /old_locus_tag="pgaptmp_005522"
     CDS             11885..12619
                     /gene="frdB"
                     /locus_tag="ACHKNB_RS27610"
                     /old_locus_tag="pgaptmp_005522"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HGU4198811.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12630..13025
                     /gene="frdC"
                     /locus_tag="ACHKNB_RS27615"
                     /old_locus_tag="pgaptmp_005523"
     CDS             12630..13025
                     /gene="frdC"
                     /locus_tag="ACHKNB_RS27615"
                     /old_locus_tag="pgaptmp_005523"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HGU4198812.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            13036..13395
                     /gene="frdD"
                     /locus_tag="ACHKNB_RS27620"
                     /old_locus_tag="pgaptmp_005524"
     CDS             13036..13395
                     /gene="frdD"
                     /locus_tag="ACHKNB_RS27620"
                     /old_locus_tag="pgaptmp_005524"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HGU4198813.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            13458..14591
                     /locus_tag="ACHKNB_RS27625"
                     /old_locus_tag="pgaptmp_005525"
     CDS             13458..14591
                     /locus_tag="ACHKNB_RS27625"
                     /old_locus_tag="pgaptmp_005525"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HGU4198814.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14680..15213
                     /gene="blc"
                     /locus_tag="ACHKNB_RS27630"
                     /old_locus_tag="pgaptmp_005526"
     CDS             14680..15213
                     /gene="blc"
                     /locus_tag="ACHKNB_RS27630"
                     /old_locus_tag="pgaptmp_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HGU4198815.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15210..15527)
                     /gene="sugE"
                     /locus_tag="ACHKNB_RS27635"
                     /old_locus_tag="pgaptmp_005527"
     CDS             complement(15210..15527)
                     /gene="sugE"
                     /locus_tag="ACHKNB_RS27635"
                     /old_locus_tag="pgaptmp_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HGU4198816.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15702..15848)
                     /gene="ecnB"
                     /locus_tag="ACHKNB_RS27640"
                     /old_locus_tag="pgaptmp_005528"
     CDS             complement(15702..15848)
                     /gene="ecnB"
                     /locus_tag="ACHKNB_RS27640"
                     /old_locus_tag="pgaptmp_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HGU4198817.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15959..16084)
                     /gene="ecnA"
                     /locus_tag="ACHKNB_RS27645"
                     /old_locus_tag="pgaptmp_005529"
     CDS             complement(15959..16084)
                     /gene="ecnA"
                     /locus_tag="ACHKNB_RS27645"
                     /old_locus_tag="pgaptmp_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HGU4198818.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16136..16702)
                     /gene="efp"
                     /locus_tag="ACHKNB_RS27650"
                     /old_locus_tag="pgaptmp_005530"
     CDS             complement(16136..16702)
                     /gene="efp"
                     /locus_tag="ACHKNB_RS27650"
                     /old_locus_tag="pgaptmp_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGU4198819.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16744..17772
                     /gene="epmB"
                     /locus_tag="ACHKNB_RS27655"
                     /old_locus_tag="pgaptmp_005531"
     CDS             16744..17772
                     /gene="epmB"
                     /locus_tag="ACHKNB_RS27655"
                     /old_locus_tag="pgaptmp_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HGU4198820.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            18162..19031
                     /locus_tag="ACHKNB_RS27660"
                     /old_locus_tag="pgaptmp_005532"
     CDS             18162..19031
                     /locus_tag="ACHKNB_RS27660"
                     /old_locus_tag="pgaptmp_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HGU4198821.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4825 a         4995 c         4879 g         4426 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcagtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaat aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacattg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccttg ctggcaggca
     2401 actatctgat ggcgaacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca ccgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgcttaaagg ccaggaaatg gggcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacct gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcatagag gcactccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattacccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga tgcgctgaat caggaaattc
     3361 tccaggtcag cagtcagttg ctggataaga gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattca ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttgcgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggt gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgtga attctccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtacctc aacaaagagg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcaccactgg ttgccattct ggcgtcagcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggtctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtga caattcgcga tctcaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gttacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctgcgccat gagatccacc agttaattct ggcaggcttc cacgcccacg
     6241 gtatcgacat gccattccca ccgttccaga tgcgtctgga aagcctgaat ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taaggtcttt
     6361 gtggccggat gaccccatcc ggctcacaaa caaaactgat agcgcagaaa aaactgccac
     6421 agccagtagc cgaaaatagc cagcgcggtg gcgtacataa accagccgcg tttttttcgc
     6481 cacagcgcga accagagaaa tgggaaaaag agcggtaacg tcattatcac accgacatcg
     6541 cggatatcgt cttcttcgag atacatcaac acatcgcaga cgttgttaat cccgcgtcca
     6601 tcaatcatcc actctttgtc cagcgcggag gtaatggtta tccagaggaa cgccacaatg
     6661 aaccagatgc agcacaagac aaacatgcgc gcactcataa ggtaaccggt cgcttaattt
     6721 cgctatagcg catatgaaac aaatcgccgt atcccactaa ttccccgcga aaaaccagac
     6781 atcggataag ataagcgcaa taaatatcca gcaggtttcc ccaccgttta ccatcgcgat
     6841 ctttgagggt gttaatggtg ctacccacca ccatcgccac tttacgggtg tggcaggcat
     6901 cgacaaaaga gagaatagtc tgctctattt ccgctacatc cccggcagaa aattgcagcg
     6961 ccctgatttc atcctcggtt attgccgggt cccggctggt gtcatccata acatttcctg
     7021 tcattgcgcc ctatcgtcag gcagacgata gggcatcggg ttattccacc gcccactgcg
     7081 gattttttgc cactctgctg caatagttct ggtaaatcgc catcgctaac agcgagaaga
     7141 agacagggcc gccaatcatc cacaatgtgc tgtcccagtc tccggcttca accacaggtt
     7201 gaatgatggt gaagacgttc gcaaatgtca ccaccagtac gacaaccact gtcgcaatca
     7261 ttgccgacaa atgcgttttg aaaatcacaa acggtctgtc gagatcctga cgtgctttaa
     7321 agaacgggaa agccagcgcg aggaacaggt aaggaagcgt catagacacg ttcgccatca
     7381 gcgtcagctt gttaaagaac gccgatgcgg taccgccacc aaacgaaacc agcaggatga
     7441 agacagtaac caacccgcac tgcatccaca tggcgataga aggcatcccc atcgcattca
     7501 ggcgcgtcat cggttccggc cacaacgctt tcggcgtccc ctggatgatg gctttcagcg
     7561 gtgaatagca aagcgtaaag aacgccccgg tataagcgag gaacatcgaa agtccggtaa
     7621 tacgcgcaaa ccatacaccc agcgacaatg acgcttcagg tgacaaatgc agtgcgttac
     7681 ccagcgtcac cccgaggctc ttcatcagca cataggtaat attgccgagg ttaacggaac
     7741 cattacttaa tacctgctgc cagtttgtgc tgacacccca taaaaatatt gccagcgaat
     7801 aaccgattga aataacaata gcggcaaaaa caataccttt ggcaaagttc ttttctggat
     7861 tttccgtttt atcgaccaga ccaccaaccg cttcaattcc gccataggca aaaatagcaa
     7921 ataccacaaa cgatagcatt gccagaccgg actgataacc tgggttcggt gatgcaagga
     7981 aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca
     8041 gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt
     8101 tattaatccc ctttgaagca acgacggtga ccagaatcat ccatgccact gccagcagac
     8161 caaccacctg cgtaggctcc agtccagcaa tacgccagtg ctgggtcatg tcgctaccat
     8221 agaggaatgt tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat
     8281 aagaggaaaa ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca
     8341 tccaggaata gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta
     8401 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
     8461 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
     8521 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
     8581 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aatattgagc aggcagcgta
     8641 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
     8701 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
     8761 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
     8821 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
     8881 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
     8941 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
     9001 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
     9061 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
     9121 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
     9181 ttgtagcagc gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc
     9241 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
     9301 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
     9361 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat
     9421 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     9481 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     9541 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     9601 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     9661 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
     9721 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     9781 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     9841 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     9901 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     9961 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
    10021 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
    10081 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
    10141 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
    10201 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
    10261 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
    10321 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
    10381 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
    10441 atgaaaatcg agcgcacttg gttcgccgcc gataagaccg gcttccatat gctgcacacg
    10501 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
    10561 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
    10621 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
    10681 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
    10741 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
    10801 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
    10861 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
    10921 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
    10981 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac
    11041 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
    11101 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
    11161 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
    11221 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa
    11281 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
    11341 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
    11401 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
    11461 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
    11521 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
    11581 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
    11641 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
    11701 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
    11761 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
    11821 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
    11881 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
    11941 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
    12001 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
    12061 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
    12121 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
    12181 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
    12241 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
    12301 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
    12361 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
    12421 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
    12481 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
    12541 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
    12601 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
    12661 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
    12721 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
    12781 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
    12841 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
    12901 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
    12961 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
    13021 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
    13081 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
    13141 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
    13201 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
    13261 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
    13321 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
    13381 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
    13441 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
    13501 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
    13561 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
    13621 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
    13681 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
    13741 ctcgagggga aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta
    13801 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat
    13861 tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
    13921 agcctgcatg ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
    13981 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct
    14041 tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt
    14101 acgcctgggg atatcgcgag ggcaaggcag ttcatgtttc gccaggggcg ttagatgcta
    14161 aaacttatgg tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga
    14221 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
    14281 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
    14341 atcctgacag catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa
    14401 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa
    14461 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc
    14521 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
    14581 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
    14641 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
    14701 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
    14761 gtaacagtag tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc
    14821 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
    14881 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag
    14941 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
    15001 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
    15061 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
    15121 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
    15181 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
    15241 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
    15301 acaatgccgg tgatagccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
    15361 cctactggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
    15421 acggtaataa cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc
    15481 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
    15541 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
    15601 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
    15661 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
    15721 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
    15781 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
    15841 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
    15901 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
    15961 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
    16021 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
    16081 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
    16141 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
    16201 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
    16261 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
    16321 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
    16381 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
    16441 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
    16501 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
    16561 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
    16621 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
    16681 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
    16741 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
    16801 gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa
    16861 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
    16921 atcgatcgca tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg
    16981 caagatgagt ttgtcgtcgc gcccggattc tccaccgacc cactggaaga acagcacagc
    17041 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
    17101 tgcgcggtaa attgccgtta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
    17161 aacaagcgta actggcagac tgctcttgag tatgttgctg ctcatccgga actggacgag
    17221 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
    17281 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
    17341 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
    17401 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
    17461 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
    17521 gtgaacgata acgcaaaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgta
    17581 atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt
    17641 gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg
    17701 ccgaaactgg cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta
    17761 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
    17821 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
    17881 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
    17941 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
    18001 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
    18061 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
    18121 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
    18181 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
    18241 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
    18301 tgattgcgct ggaaagtcgt atgcatcaaa agcataaact ggatgctgct gcacgccttc
    18361 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    18421 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    18481 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    18541 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    18601 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    18661 atgacgttga ctgccaggaa aatggcaatc tggaatatga cacctattcc caaccggaat
    18721 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    18781 gcaaagaaca atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    18841 tagaggcgat cactcgccga atgcttgatt tcagccaacg actgaaaaaa ctcgcgggtg
    18901 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    18961 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    19021 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    19081 ataacgtgat cgttggtcat gccggatgcg gcgtaaacgc cttat
//
LOCUS       DAZFVO020000583         6925 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.218, whole genome shotgun sequence.
ACCESSION   DAZFVO020000583 DAZFVO020000000
VERSION     DAZFVO020000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.218"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            105..1244
                     /locus_tag="ACHKNB_RS27665"
                     /old_locus_tag="pgaptmp_005533"
     CDS             105..1244
                     /locus_tag="ACHKNB_RS27665"
                     /old_locus_tag="pgaptmp_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HGU4198822.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1241..1855
                     /gene="prfH"
                     /locus_tag="ACHKNB_RS27670"
                     /old_locus_tag="pgaptmp_005534"
     CDS             1241..1855
                     /gene="prfH"
                     /locus_tag="ACHKNB_RS27670"
                     /old_locus_tag="pgaptmp_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HGU4198823.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1912..3369)
                     /gene="pepD"
                     /locus_tag="ACHKNB_RS27675"
                     /old_locus_tag="pgaptmp_005535"
     CDS             complement(1912..3369)
                     /gene="pepD"
                     /locus_tag="ACHKNB_RS27675"
                     /old_locus_tag="pgaptmp_005535"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HGU4198824.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3630..4088
                     /gene="gpt"
                     /locus_tag="ACHKNB_RS27680"
                     /old_locus_tag="pgaptmp_005536"
     CDS             3630..4088
                     /gene="gpt"
                     /locus_tag="ACHKNB_RS27680"
                     /old_locus_tag="pgaptmp_005536"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGU4198825.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4180..5424
                     /gene="frsA"
                     /locus_tag="ACHKNB_RS27685"
                     /old_locus_tag="pgaptmp_005537"
     CDS             4180..5424
                     /gene="frsA"
                     /locus_tag="ACHKNB_RS27685"
                     /old_locus_tag="pgaptmp_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HGU4198826.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5482..5883
                     /gene="crl"
                     /locus_tag="ACHKNB_RS27690"
                     /old_locus_tag="pgaptmp_005538"
     CDS             5482..5883
                     /gene="crl"
                     /locus_tag="ACHKNB_RS27690"
                     /old_locus_tag="pgaptmp_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HGU4198827.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5922..>6925)
                     /gene="phoE"
                     /locus_tag="ACHKNB_RS27695"
                     /old_locus_tag="pgaptmp_005539"
     CDS             complement(5922..>6925)
                     /gene="phoE"
                     /locus_tag="ACHKNB_RS27695"
                     /old_locus_tag="pgaptmp_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HGU4198828.1"
                     /translation="VQAAEIYNKDGNKLDVYGKVKAMHYMSDNDSKDGDQSYIRFGFK
                     GETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALY
                     DVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNEN
                     RDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLK
                     YDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDI
                     EGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT         1674 a         1695 c         1794 g         1762 t
ORIGIN      
        1 ctggattcgt ctgccatttc ctgatttttt aattgttact ctcaataatt caagtcgcaa
       61 cacatgcaac ttgaagtatg acgagtctat ttacggaatg gtatatgggc aattatattc
      121 gtcccttatc cgatgcggta tttaccatcg catctgatga cctgtggatc gagagtttag
      181 cgatccaaca attacacacc acggcaaatt tacccaacat gcagcgcgta gtggggatgc
      241 cagatttaca tcccggacgc ggctatccga ttggcgcagc gttcttctct gttggtcgtt
      301 tttatccggc actggtcggc aatgatatcg gctgcggtat ggcgctatgg caaacagata
      361 ttctcactcg caaatacaac gccgataagt ttgaaaagcg attatcagat ctggatgacg
      421 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggc
      481 gcagctcgct tggctctatc ggtggcggca atcatttcgc tgaactgcaa caagttgatc
      541 aaatcatcaa cgctgaactg tttgcactgg caggtctgga tgcacaacat ctgcaactgt
      601 tggttcatag cggctcgcgg ggggtggggc agtctatttt acagcggcat attgcctcgt
      661 tctcgcatca tggtttgcct caaggtagtg acgacgcgct acggtatatt gcggaacatg
      721 acgatgcgct ggcgtttgcg tgtattaatc gccagatgat cgctttgcgc ataatgcaac
      781 aagttaaggc cacgggaagt ccggttctgg atgtggcgca taactttgtt agcgcgtgtc
      841 aaatcggcga tcaacagggc tggttgcatc gtaaaggtgc cacgccggat gacaacggtc
      901 tggtgattat tcccggttca cgtggtgatt actcctggat tgtacagcct gttgcgaacg
      961 aaaaaacgct ccactcgctg gcgcatgggg cagggcgtaa atgggggcgc accgagtgta
     1021 aagggcgtct ggcagcgaaa tacacggcga cgcaactctc gcgtactgaa cttggcagtc
     1081 gggtaatttg tcgcgataaa caactcatct ttgaagaagc gccacaagcc tataaatcgg
     1141 ctgaaagcgt ggtgcaatgt ctggtgcagg ccggtttaat tattcctgtc gcgcggctgc
     1201 gtccggtgct aacgctcaaa aacagtggag ggaaaaaagg atgatcttgc tacaactctc
     1261 ctcagcgcag gggccggaag aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat
     1321 taaagaagct gcccgacaag acgtcgcggt aacggtgctg gaaacagaaa cgggccacta
     1381 ctctgacacg ctgcgttcgg cgctgatttc tctggatggc gataacgcat gggcattaag
     1441 cgaaagctgg tgtggcacta ttcagtggat ttgtccgagt ccgtatcggc ctcatcatgg
     1501 gcgcaaaaac tggtttctgg gcattgggcg ttttaccgct gatgagcagg aacaatcgga
     1561 tgcaatccgt tatgagacgt tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac
     1621 cgactcggcg gtacgcgcca cgcatttggc atccggtatt agcgtgaagg ttcagtcaga
     1681 gcgtagtcag catgctaaca agcggctggc acgattgctg attgcctgga agctggagca
     1741 gcagcaacag gaaaatagcg cggtgctgaa atcgcagcgg cgaatgttcc atcaccagat
     1801 tgaacgtggc aacccgagac ggacatttac agggatggct tttatcgaag gataatgaag
     1861 gatgaaactg ccggaaggcg attaaacgcc atccggcagc aaatcaaata attacttcgc
     1921 cggaatttct ttcagcagtt cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg
     1981 aacttgctca tccggagagt gtggaccggt gatagttggc ccgatagaaa ccatgtccat
     2041 ttccggatac ggttttttga acagaccaca ttccaggccc gcgtggataa tctggatgtt
     2101 cggcgttttg ttgaacagac gctgataggt ttcacgtacc agatgcatca ccggagaatt
     2161 agcgtccggc tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt
     2221 acccagcgaa tccagcatgc tcaccacgta gtctttaccg ctgtcgatca gtgaacggat
     2281 caggcagtga atttctacgt tattgtcagt catggtcacc acaccgacgt tcagggaggt
     2341 ttcgaccaca ccttttgcca cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag
     2401 cagacgaata aaggtatcgc gagatttcgc aatcagggca gctttatcgt tcgctacaga
     2461 gtccagcaac aaggccagat ttttctcttt ctctgccagc tcgtttttca ggatctcctg
     2521 ataggtattc accagagatt tcaggacgtc gactttatca gctgcgacag caatggtcgc
     2581 aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcga taaggcgtag
     2641 atccagttct tccgcatgac ccgccaggaa gcgcaccagt agtttgttgg cattacccag
     2701 gccaacgtgg atttcaccgc cggagtgacc gcctttcaga ccttttaagg ttaacttgaa
     2761 ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat
     2821 accccccgca caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat
     2881 atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg tccataccgg cttcttcggt
     2941 catggtcagc agcacttcca gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag
     3001 cgcagacgcc ataccaatgc cgttatctgc acccagcgtg gtgccgcgcg ctttaaccca
     3061 ttcgccatca atataaggct ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt
     3121 tttctgcggc accatatcga ggtgggcctg taagacgacc ggtttacgat tttccatacc
     3181 tgcggtagca ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc
     3241 tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg
     3301 aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag
     3361 ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt
     3421 tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtttgaga ataccacttt
     3481 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     3541 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     3601 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3661 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3721 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     3781 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3841 ttaaagtgct aaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3901 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagca cattttgtga
     3961 ctatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     4021 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     4081 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     4141 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     4201 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     4261 aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg
     4321 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg tgaaatcctc
     4381 gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgatgatga tttatacgat
     4441 accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg
     4501 tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca gtggtcgaca ctggctgcat
     4561 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     4621 caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg
     4681 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     4741 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     4801 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact
     4861 attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg
     4921 ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     4981 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg
     5041 ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     5101 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg
     5161 catgatgctt ccgatgacgc gttgcgcgtg gagctgaatc gctattcatt aaaagtgcaa
     5221 ggattgctgg gacgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     5281 ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta
     5341 gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc
     5401 tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt
     5461 gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc
     5521 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc
     5581 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc
     5641 tggggctggt ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt
     5701 ggtctgttcg ataaagcagg cgactggaag agcgttccgg taaaagacac tgaagtggtt
     5761 gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg
     5821 ctgaatctga agctggaacc ggcggatgat tttcgtgacg agccggtgaa gttaacggcg
     5881 tgagtgaaat gtgccggatg catcacatcc ggcaataatc attaaaactg ataggtcatg
     5941 ccaaccgcga caatatcatc attattaata ttcaatttgt tatcgctatc cagttggttg
     6001 attttataat caacaaacgc tgacatattt ttgttgaaat aatatgtagc cccgacatcg
     6061 atataattga ccagatcttc atcaccgata ccttcaatat ctttcccttt cgataagaca
     6121 taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc
     6181 tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaataaaaa
     6241 gttgccagat aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc
     6301 ttgccagtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggca
     6361 ccactaatgg cgaaatcgct gccaccaaag tcatatgtca atgacgtgcc gaagccatcg
     6421 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
     6481 aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
     6541 ttggtcataa agttgtcggt ctgcgccgag gagtcgccac caaattccgg gaacatatcg
     6601 gtccaggctt ccacgtcata caacgcgccc aggttacggc catagtcgaa agaacccaaa
     6661 tctttatact tcaatccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc
     6721 gctttatttc cggcaaactc cgcttcccaa cggccatagc cagtcagttg atcgttaatt
     6781 tgtgtttcgc ctttaaaacc aaaacggata taactctggt cgccatcttt actgtcgtta
     6841 tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt accgtctttg
     6901 ttatatattt ctgcggcctg tacgg
//
LOCUS       DAZFVO020000584         1639 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.346, whole genome shotgun sequence.
ACCESSION   DAZFVO020000584 DAZFVO020000000
VERSION     DAZFVO020000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.346"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            268..1215
                     /gene="stxA1a"
                     /locus_tag="ACHKNB_RS27700"
                     /old_locus_tag="pgaptmp_005540"
     CDS             268..1215
                     /gene="stxA1a"
                     /locus_tag="ACHKNB_RS27700"
                     /old_locus_tag="pgaptmp_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HGU4198829.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRGRGITHNKILWDSSTLGAILMRRTISS"
     gene            1225..1494
                     /gene="stxB1a"
                     /locus_tag="ACHKNB_RS27705"
                     /old_locus_tag="pgaptmp_005541"
     CDS             1225..1494
                     /gene="stxB1a"
                     /locus_tag="ACHKNB_RS27705"
                     /old_locus_tag="pgaptmp_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HGU4198830.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          439 a          280 c          397 g          523 t
ORIGIN      
        1 tctacgagtt tgccagcctc ccccagtggc tggctttttt atgtccgtaa catcctgtgt
       61 atcaataaat gttgttgtct acgtacgtca agtagtcgca tgagatctga ccagatatgt
      121 taaggttgca gctctctttg aatataatta tcattttcat tacgttattg ttacgtttat
      181 ccggtgcgcc gtaaaacgcc gtccttcagg gggtggagga tgtcaagaat atagttatcg
      241 tatggtgctc aaggagtatt gtgtaatatg aaaataatta tttttagagt gctaactttt
      301 ttctttgtta tcttttcagt taatgtggtt gcgaaggaat ttaccttaga cttctcgact
      361 gcaaagacgt atgtagattc gctgaatgtc attcgctctg caataggtac tccattacag
      421 actatttcat caggaggtac gtctttactg atgattgata gtggcacagg ggataatttg
      481 tttgcagttg atgtcagagg gatagatcca gaggaagggc ggtttaataa tctacggctt
      541 attgttgaac gaaataattt atatgtgaca ggatttgtta acaggacaaa taatgttttt
      601 tatcgctttg ctgatttttc acatgttacc tttccaggta caacagcggt tacattgtct
      661 ggtgacagta gctataccac gttacagcgt gttgcaggga tcagtcgtac ggggatgcag
      721 ataaatcgcc attcgttgac tacttcttat ctggatttaa tgtcgcatag tggaacctca
      781 ctgacgcagt ctgtggcaag agcgatgtta cggtttgtta ctgttacagc tgaagcttta
      841 cgttttcggc aaatacagag gggatttcgt acaacactgg atgatctcag tgggcgttct
      901 tatgtaatga ctgctgaaga tgttgatctt acattgaact ggggaaggtt gagtagtgtc
      961 ctgcctgact atcatggaca agactctgtt cgtgtaggaa gaatttcttt tggaagcatt
     1021 aatgcaattc tgggaagcgt ggcattaata ctgaattgtc atcatcatgc atcgcgagtt
     1081 gccagaatgg catctgatga gtttccttct atgtgtccgg cagatggaag aggccgtggg
     1141 attacgcaca ataaaatatt gtgggattca tccactctgg gggcaattct gatgcgcaga
     1201 actattagca gttgaggggg taaaatgaaa aaaacattat taatagctgc atcgctttca
     1261 tttttttcag caagtgcgct ggcgacgcct gattgtgtaa ctggaaaggt ggagtataca
     1321 aaatataatg atgacgatac ctttacagtt aaagtgggtg ataaagaatt atttaccaac
     1381 agatggaatc ttcagtctct tcttctcagt gcgcaaatta cggggatgac tgtaaccatt
     1441 aaaactaatg cctgtcataa tggaggggga ttcagcgaag ttatttttcg ttgactcaga
     1501 atagctcagt gaaaatagca ggcggagatt cataaatgtt aaatacatct caattcagtc
     1561 agttgttgcc ggtctgataa tagatgtgtt agaaaatttc tgcatggtga atccccctgt
     1621 gcggaggggc gactggtga
//
LOCUS       DAZFVO020000585         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.350, whole genome shotgun sequence.
ACCESSION   DAZFVO020000585 DAZFVO020000000
VERSION     DAZFVO020000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.350"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27710"
                     /old_locus_tag="pgaptmp_005542"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27710"
                     /old_locus_tag="pgaptmp_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4198831.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECHITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27715"
                     /old_locus_tag="pgaptmp_005543"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27715"
                     /old_locus_tag="pgaptmp_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4198832.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          436 a          320 c          385 g          453 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt atcgtattac ttcagccaaa
       61 aggaatacct gtatatgaag tgtatattgt taaaatgggt actgtgcctg ttactgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaattgtata cggacagaaa tatcgacccc acttgaacat atatctcagg
      241 ggaccacatc ggtatctgtt attaaccaca ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttcag
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttctgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc gccgtgaatg
      901 aagagataca accagaatgt catataactg gcgacaggcc agttataagg ataaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacaggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaagc
     1321 attgctggtt tcgtagtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
     1381 cagttgacgg ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
     1441 cctgtgcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
     1501 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
     1561 ttaggcatat atccaggctc ctcatcgcag gagc
//
LOCUS       DAZFVO020000586         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.351, whole genome shotgun sequence.
ACCESSION   DAZFVO020000586 DAZFVO020000000
VERSION     DAZFVO020000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.351"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27720"
                     /old_locus_tag="pgaptmp_005544"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27720"
                     /old_locus_tag="pgaptmp_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4198833.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNCIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27725"
                     /old_locus_tag="pgaptmp_005545"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27725"
                     /old_locus_tag="pgaptmp_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4198834.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          434 a          321 c          386 g          453 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt atcgtattac ttcagccaaa
       61 aggaatacct gtatatgaag tgtatattgt taaaatgggt actgtgcctg ttactgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaattgtata cggacagaaa tatcgacccc acttgaacat atatctcagg
      241 ggaccacatc ggtatctgtt attaaccaca ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt attgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttcag
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttctgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc tccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ctaaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacgggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaagc
     1321 attgctggtt tcgtagtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
     1381 cagttgacgg ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
     1441 cctgtgcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
     1501 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
     1561 ttaggcatat atccaggctc ctcatcgcag gagc
//
LOCUS       DAZFVO020000587         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.352, whole genome shotgun sequence.
ACCESSION   DAZFVO020000587 DAZFVO020000000
VERSION     DAZFVO020000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.352"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27730"
                     /old_locus_tag="pgaptmp_005546"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27730"
                     /old_locus_tag="pgaptmp_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4198835.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     EIQPECHITGDRPVIRINNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27735"
                     /old_locus_tag="pgaptmp_005547"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27735"
                     /old_locus_tag="pgaptmp_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4198836.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          431 a          319 c          389 g          455 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt gccgtattac ttcagccaaa
       61 aggaacacct gtatatgaag tgtatattgt taaaatggat actgtgcctg ttgctgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaatagtata cggacagaaa tatcgactcc tcttgagcat atatctcagg
      241 ggaccacatc ggtgtctgtt attaaccata ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt gttgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttctg
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttttgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc gccgtgaatg
      901 aagagataca accagaatgt catataactg gcgacaggcc agttataagg ataaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacaggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaagc
     1321 attgctggtt tcgtagtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
     1381 cagttgacgg ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
     1441 cctgtgcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
     1501 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
     1561 ttaggcatat atccaggctc ctcatcgcag gagc
//
LOCUS       DAZFVO020000588         1594 bp    DNA     linear   BCT 13-FEB-2025
DEFINITION  TPA_asm: Escherichia coli isolate 2209T7962
            SAMN43283495-rid25107023.guided.353, whole genome shotgun sequence.
ACCESSION   DAZFVO020000588 DAZFVO020000000
VERSION     DAZFVO020000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283495
            Sequence Read Archive: SRR30325326
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
REFERENCE   3  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-FEB-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351588.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351588.2
            Genome Coverage       :: 141x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/06/2025 16:18:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,549
            CDSs (total)                      :: 5,456
            Genes (coding)                    :: 5,247
            CDSs (with protein)               :: 5,247
            Genes (RNA)                       :: 93
            rRNAs                             :: 3, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 99 of 209
            Pseudo Genes (incomplete)         :: 105 of 209
            Pseudo Genes (internal stop)      :: 49 of 209
            Pseudo Genes (multiple problems)  :: 38 of 209
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283495-rid25107023.guided.353"
                     /serotype="O 91:H14"
                     /isolate="2209T7962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2022-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2b"
     gene            75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27740"
                     /old_locus_tag="pgaptmp_005548"
     CDS             75..1034
                     /gene="stxA2b"
                     /locus_tag="ACHKNB_RS27740"
                     /old_locus_tag="pgaptmp_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HGU4198837.1"
                     /translation="MKCILLKWILCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIVEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27745"
                     /old_locus_tag="pgaptmp_005549"
     CDS             1047..1310
                     /gene="stxB2b"
                     /locus_tag="ACHKNB_RS27745"
                     /old_locus_tag="pgaptmp_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HGU4198838.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          429 a          320 c          390 g          455 t
ORIGIN      
        1 cgcttttgcg ggcctttttt atatctgcgc cgggtctggt gccgtattac ttcagccaaa
       61 aggaacacct gtatatgaag tgtatattgt taaaatggat actgtgcctg ttgctgggct
      121 tttcttcggt atcctattcc cgggaattta cgatagactt ttcgactcaa caaagttatg
      181 tctcttcgtt aaatagtata cggacagaaa tatcgactcc tcttgagcat atatctcagg
      241 ggaccacatc ggtgtctgtt attaaccata ccccaccggg cagttatttt gctgtggata
      301 tacgagggct tgatgtctat caggcgcgtt ttgaccatct tcgtctgatt gttgagcaaa
      361 ataatttata tgtggccgga ttcgttaata cggcaacaaa tactttctac agattttctg
      421 attttgcaca tatatcagtg cccggtgtga caactgtttc catgacaacg gacagcagtt
      481 ataccactct gcaacgtgtc gcagcgctgg aacgttccgg aatgcaaatc agtcgtcact
      541 cactggtttc atcatatctg gcgttaatgg agtttagtgg aaatgccatg accagagatg
      601 catccagagc agttttgcgt tttgtcactg tcacagcaga agccttacgg ttcaggcaaa
      661 tacagagaga atttcgtctg gcactgtctg aaactgctcc tgtttatacg atgacaccgg
      721 aagaagtgga cctcacactg aactggggga gaatcagcaa tgtgcttccg gagtttcggg
      781 gagagggtgg tgtcagagtg gggcgaatat cctttaataa tatatcagcg atactgggca
      841 cagtggcggt tatactgaat tgccatcatc agggggcacg ttccgttcgc tccgtgaatg
      901 aagagataca accagaatgt cagataactg gcgacaggcc agttataagg ctaaacaata
      961 ctttatggga aagtaatacc gcagctgctt ttctgaatcg cagggctcac tctttaaata
     1021 catccggaga ataacgggag ttaaatatga agaagatatt tgtagcggct ttatttgctt
     1081 ttgtttctgt taatgcaatg gcagctgatt gtgcaaaagg taaaattgag ttctctaagt
     1141 ataatgagaa tgatacattc acagtaaaag tggccgggaa agagtactgg actaaccgct
     1201 ggaatctgca accgctactg caaagcgcac agttaacagg aatgacggta acaatcaaat
     1261 caaatacctg tgcgtcaggt tcaggatttg ctgaagtgca gtttaattaa tatcagaagc
     1321 attgctggtt tcgtagtgtg cagcaatgta gttacagtgt aatcaatgtc acaattcagt
     1381 cagttgacgg ttgcctgtct ggctgagcat ttgttaaaaa aatcctgcat gatgaatccc
     1441 cctgtgcgga ggggcataat gacagatgtt tggttgcgta ttgtataggc aagttgcgga
     1501 ttctgtctgg tcattgcaga attcaccggg aggcacccgg catcatgctg tatacagaga
     1561 ttaggcatat atccaggctc ctcatcgcag gagc
//
